LOCUS       DAOOER010000001        80335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_297_length_80335_cov_8.453625, whole genome shotgun sequence.
ACCESSION   DAOOER010000001 DAOOER010000000
VERSION     DAOOER010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 80335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 80335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80335
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_297_length_80335_cov_8.453625"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..758)
                     /locus_tag="PL162_00005"
     CDS             complement(12..758)
                     /locus_tag="PL162_00005"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HQF90441.1"
                     /translation="MSSKIRVYELAKILGVSNKELMGVLKQLEIEVSSHMSSIDTELG
                     QKAEDFLKRSGAPVVSEAPASGQSLVVPLESTVAEVASLLRVTPGEAVKALVTAGMMI
                     PANAKVDERALDALGKAFGVSLGWAAVAPEESTEEKKTSSRTSKADEGKHVQQRPPIV
                     TVMGHVDHGKTTLLDCIRKTNVSASEAGGITQHIGASVVEFNGEKIVFLDTPGHEAFT
                     AMRARGAQVTDIAILVVAADDGLSPKPWKP"
     gene            complement(751..1083)
                     /locus_tag="PL162_00010"
     CDS             complement(751..1083)
                     /locus_tag="PL162_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90442.1"
                     /translation="MDVLDELFPLVMLARRAGVLVLGQDGIRQALRCGKNMTLLLASD
                     ASGNVRRMLRGYEERGRCAVFVLGASREMIEGRTGLPGSQIVGLPRDHGLAQRVESLL
                     SKGGEGNE"
     gene            complement(1090..1386)
                     /locus_tag="PL162_00015"
     CDS             complement(1090..1386)
                     /locus_tag="PL162_00015"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HQF90443.1"
                     /translation="MGGPGEIKRRRPRTCVGCGGEAPRSALVRIVRSPGGVVAVDRTG
                     KASGRGVYLCRRRPCLEAARKKDALSRSLKVKVPEEIYLALEALCVGGTIEGEV"
     gene            complement(1365..2489)
                     /gene="nusA"
                     /locus_tag="PL162_00020"
     CDS             complement(1365..2489)
                     /gene="nusA"
                     /locus_tag="PL162_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HQF90444.1"
                     /translation="MQLGRDFVKALHQIEEEKGLPVGVITSSLEAALISAYRKHRGGN
                     QNVEVNLDLEREEISLFEVRRVVAEGSDPDTEMSLTEARLLGYHDVDPGDVVRIEVFP
                     EGFGRIAAQTARQVIIQRLKDAERQVIFDEFADKIGDLVTGVIFKTEGEHVLVRLNER
                     TEAILPREERIVGESYPPGERFKFFLLDVRRTTRGPRIVVSRTHPGLLRRLLEVEVPE
                     IAEGVVEIRNIVRESGTRSKVAVLSMDANVDPVGACVGPKGARIKAVGGELGDERMDI
                     IIWSADPLQYIRNALSPAKVVRVEPSLEADRAARVFVRPDQLSLAIGKAGQNVRLAAR
                     LTGWKIDIKVLEPERMPTLQDLFEDLIKGAPKELWEDREK"
     gene            complement(2473..2994)
                     /gene="rimP"
                     /locus_tag="PL162_00025"
     CDS             complement(2473..2994)
                     /gene="rimP"
                     /locus_tag="PL162_00025"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HQF90445.1"
                     /translation="MVGHKRRPPLPTDLVREAVEGLGYEFVGMERVVEGDQSVVRLYI
                     DSLGGINVKDCETVSRKVSPLFDGPEDLFEGRYFLEVSSPGVERPLFSQKDFERFLGQ
                     RAQLSHRDGAGKIVKCTGTLSHVTEDAVSLRRDDGVEVVISLADMGRAHLVYEPEKPS
                     KPGKGGTARAARS"
     gene            complement(3251..3439)
                     /locus_tag="PL162_00030"
     CDS             complement(3251..3439)
                     /locus_tag="PL162_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90446.1"
                     /translation="MRGPSLLSLTGEERGVVWQGILELSRLEEEVLGHDQLQPSLEET
                     FSSGMDEWWPVFVSRDCM"
     gene            complement(3471..4946)
                     /gene="guaB"
                     /locus_tag="PL162_00035"
     CDS             complement(3471..4946)
                     /gene="guaB"
                     /locus_tag="PL162_00035"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HQF90447.1"
                     /translation="MSEQSKFASYEGVTFDDVLLVPGYSEVVPAQVDLRTRLTPDIEL
                     NLPLCSAAMDTVTESRLAIAMAREGGIGVIHRNMPKERQAAEVDKVKRSESGVIVDPF
                     FLHPENLLRDAVGLMEHYHISGVPIVDASRRLVGIITNRDLRFVTDYDQPIQEVMTKD
                     RLLTAPEGTTLEDAKSILMSHKVEKLPIVDGHYRLKGLITIKDIQKAKDFPNASKDSH
                     GRLRVAAAVGAGSDTLERVEMLARAGVDALVVDTAHGHSAGVIRKVKELRSLFSDLPI
                     VAGNVATTEAAEALMDAGADGIKVGVGPGSICTTRIVAGIGVPQITAVMEAARATKSR
                     GRCVIADGGVRYSGDVVKALAAGADTVMVGALLAGTEESPGETVIYRGRSFKSYRGMG
                     SLGAMKEGCSKDRYFQEGVFEEKLVPEGIEGMVPCKGSLGAVLYQLAGGLRAGMGYVG
                     AATVSELKEKGRFVKITAAAMRESHPHDVTITKEAPNYGVE"
     gene            complement(5097..6266)
                     /gene="ispF"
                     /locus_tag="PL162_00040"
     CDS             complement(5097..6266)
                     /gene="ispF"
                     /locus_tag="PL162_00040"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HQF90448.1"
                     /translation="MKDWSLIVVAAGRGSRMGGVPKQFRPLGGYPLWHWSLALAEALH
                     AAGDLRECVLVVPPADPGVETTPPPGGVVPVVRVEGGAERGDSVLAGVRAASGAWVMV
                     HDAARPFCSLELCRRLQAAAEASGGAIPLVSVADALKKVSGGTVTPLPREGVFGTQTP
                     QAFFRERLLEALERSPCTVRDEAEAWIAAGHNLTPVEGERENFKLTYEGDWHMAELMV
                     APPQKFRVGHGFDIHPLVPERRLILAGMTIPSPLGLDGHSDADLVAHAVADALLGAAG
                     LPDLGRLFPASDQRYKGARSMDLLGRVVDYVRDEGWEPEWVDVTLEAQLPRLGPLCDA
                     MGVALDDVLRCGEGPCCHVKVKSAEKIGALGRGEALACHAVALLGRRKSPRTGEQ"
     gene            complement(6263..7456)
                     /locus_tag="PL162_00045"
     CDS             complement(6263..7456)
                     /locus_tag="PL162_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HQF90449.1"
                     /translation="MTRGGGYMGENSGALGRLARELFRWFLAIMGGIAGYQVTEFIQS
                     AGWWPFQTILMSLAGTGSFVVVFGGVSYLIAPLFHRGILRLGSLLEGHLQNISWINIS
                     VGLIGLIVGLLLANLLALPFVDLPVLGRYLAILLNVALGYAMMWLFIKRRDDMVAMMG
                     SVGGIWRDRQGEPRKRQGKYATMDLQGGTLSPLACPAKLLDTSVVIDGRILDVARTGF
                     VEGALTLPRFVLVELQGIADSSDPVRRGRGRRGLDVINGLQKIPTIRLEILELSLKDL
                     NAETVDLALIQLARDMGGKILTTDYNLNKISQIQGIDVLNVNDLANALKPMLLPGESL
                     MVDVLREGKEQSQGVGYLDDGTMIVVEDGGRAVGRRVEVTVTSMLQTSAGRMIFGRIR
                     RESAA"
     gene            complement(7470..8096)
                     /gene="recR"
                     /locus_tag="PL162_00050"
     CDS             complement(7470..8096)
                     /gene="recR"
                     /locus_tag="PL162_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HQF90450.1"
                     /translation="MVLPEALTRLTTFLQRLPGVGEKSARRMAFFLFQQDPSFADQLA
                     EALVTLRRSLHPCPQCGNLTDGDCCGVCGDPMRERDTLCVVESVEDLMSLEQAGIFSG
                     QYFVLGGRVSPLEGEEISPETLERLRGRIRDLRVREVVVATNPRIEGDLACHVVVDAL
                     ADVPVRITRLAYGLPVGGSIGFADRVTLHAALESRLVVRSGEASPGPD"
     gene            complement(8100..8414)
                     /locus_tag="PL162_00055"
     CDS             complement(8100..8414)
                     /locus_tag="PL162_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HQF90451.1"
                     /translation="MKMDKILKQAQKMQAQMLQMQEELAKVTVEGAAGGGMVRAVANA
                     QGEVLSVKIAPEVVDPQEIDMLEDLVLAAVNEALRLGKEKANRQMEQVTGGMGLSMPG
                     LF"
     gene            complement(8476..8937)
                     /locus_tag="PL162_00060"
     CDS             complement(8476..8937)
                     /locus_tag="PL162_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HQF90452.1"
                     /translation="MVMITVGMPRARWVEEAGQEYVRRLSRWAPIAWEHVAEAHHRPS
                     GSKGVEEEGLRLLRSFQPRDVVVLLDEGGRSFSSVSFSQWLFEIYEGSPGRLAFVVGG
                     AYGVSSAVRERAAFCLSLSSLTFPHELCAVVLLEQIYRAVTLRHHIPYHHA"
     gene            complement(8940..9587)
                     /locus_tag="PL162_00065"
     CDS             complement(8940..9587)
                     /locus_tag="PL162_00065"
                     /inference="COORDINATES: protein motif:HMM:NF013935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HQF90453.1"
                     /translation="MALPATREQLLLLRQQMEAVRRGKALLERKRDALLRALEEDRRR
                     FRSLQEQFDRVGRRVATTYSLLRLYEGQISLDLLRPGMGPVPLASFRHVLMGCAFVEF
                     RRQGDESRPVSYDPALASIFTDDLVGALAELDHIFWTYINLRVKMGALEKALRDTVRK
                     TSNLEHRVLPGLREDFGRIRDVLVERERQERYTLRKALRRQKTTEPPGAQAGFSP"
     gene            complement(9590..10990)
                     /locus_tag="PL162_00070"
     CDS             complement(9590..10990)
                     /locus_tag="PL162_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HQF90454.1"
                     /translation="MTSGQEALYREGYRSVCGISGPLIFVQGVRDAGYGELVTIETPE
                     GPRSGQILQVDRDTAVIQVFEGTTGLDESSTTIWLEKSVLTVPVGPGLMGKVLTGRGH
                     LLEGGEVPYAEGRMSVQGLALNPARRKAPNAYVETGISTIDVMNTLVRGQKLPVFTGA
                     GLPANRLAAQIAAGARVPGTGRPFLVVFAAMGITRREADFFLSAFQDSGALAKGVFFM
                     NLAGDSAVERVLTPRIALTTAEYFAFTLGYDVLVILTDMLSYCDALREVSAAREEIPG
                     RRGYPGYMYSDLASLYERSGCVEGLAGSVTQIPILTMPDDDMTHPVVDLSGYITEGQV
                     VLSRRLADRGIYPPVQVLPSLSRLMNKGIGKGRTVPEHRAMADQLYAAYAKAQDIERL
                     RLITGDEGLSSTERSYLVFGKSFEERFIGQPEGPRTLERSLEAAWQCLRDLPASELYR
                     LPRDLVREILGEKGGR"
     gene            complement(10987..12747)
                     /locus_tag="PL162_00075"
     CDS             complement(10987..12747)
                     /locus_tag="PL162_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HQF90455.1"
                     /translation="MGHSGSILSVSGPVVRARPQGPVRMFEVARVGQEELIGEVVRLR
                     GDVVDIQVYEETMGLRTGEPVWFSGELLSVELGPGLLGNVLDGIGRPLVPLGREGIFL
                     PRGARRDTLDREASWAFTPKVTPGDRVDPGDILGHVVEGADFLHAIMVPPGARGGTVE
                     SVAPAGSFSPDATVCTTREGENYALFHPWPVRQPRPVRRYLPLENPLETGQRVLDTLF
                     PLALGGAGVIPGGFGTGKTVTQQSLARWCNADVIVYIGCGERGNEMTEVLEEFPELMD
                     PRTGRPLMERVVLVANTSNMPVAAREASIYLGMSIAEYFRDMGRHVAIMADSLSRWAE
                     ALREIGGRLEEMPGEEGYPAYLPSRLSQYFERSGRVVAQGTPEREGSVTVISAVSPPG
                     GDFSEPVTQSCLRLSGAFWGLDKTLAQQRHFPAINWRSSYTLYEDSLAHHFLKALGED
                     WEGKRHFLRTTLDREKELLELVQLVGRDGLSEEDKWLLELAELLRIVFLQQNAFDAVD
                     AFTSLRKAKALLDIFRNLDQEVVQRLRQGLGTDQVPRTFLRDELFRLRSLEEEGLASE
                     GELFIQSLKAQLASEEVGPA"
     gene            complement(12757..13014)
                     /locus_tag="PL162_00080"
     CDS             complement(12757..13014)
                     /locus_tag="PL162_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HQF90456.1"
                     /translation="MKEFGVALGRKIFVDLWALNGLRPICCDVPGDLAEVWPELTAGD
                     VCLVVVEEGWFAQLAPPWRRKILAQVRPAWIPVPDMISQGL"
     gene            complement(13011..14078)
                     /locus_tag="PL162_00085"
     CDS             complement(13011..14078)
                     /locus_tag="PL162_00085"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HQF90457.1"
                     /translation="MARKTTSSDSPENYAYINARLRSRGARFFSSEELRRLADGVLED
                     LEVALLDGRYAEVLRSRLVTSERSAFRRVEKTLSLGTAQSFDVLRRRAEGETAVLVDI
                     LLARADFQNARLMLRSMTSPHRSPKEMPFWASFGVLSPALFGELWESHDLQEFASRCR
                     GIPHPFGGVLWEASQDLMRHGSLPRAERHLLLGYLEFLRRALDPWKSSGADRVREYLG
                     RLADVWNSGIWLRERAGYLSASESVQAYVPGGACLSLDRLRRSSTLEALVSHTCWRDV
                     ARRGAPFSPHRTQQRFAESFLRWQASLFRKDLLGIDVGIGFIGLQLLEEQNLNRVAVG
                     MALGLPSRRIQETLLFMEPDA"
     gene            complement(14026..14646)
                     /locus_tag="PL162_00090"
     CDS             complement(14026..14646)
                     /locus_tag="PL162_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90458.1"
                     /translation="MKDSLVAFKEQMRREHEERKAAFRREAEERISDMIASRRSQAEK
                     DITRWRQDRREVHERQEQILRQRALGRYRHLRMEVVRELVARVMAQVEFRFQNADAEE
                     RRRWLILLAQEALGCMRGKAVLAVPPGDEDAVRELPGVERVEGIVKDPWGGCVSRDAA
                     DGRHMVDNTFRTRWSRSEAFFAAQLGMALGGQEDDVERFARELRLY"
     gene            complement(14668..14886)
                     /locus_tag="PL162_00095"
     CDS             complement(14668..14886)
                     /locus_tag="PL162_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HQF90459.1"
                     /translation="MEKALIAFAATLAIGLPALATGYAQAKIGSAAAGTVAEKPETAG
                     TMIILEAIPETMVILGFVVAVMVLVKLG"
     gene            complement(14868..16790)
                     /locus_tag="PL162_00100"
     CDS             complement(14868..16790)
                     /locus_tag="PL162_00100"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HQF90460.1"
                     /translation="MIRRMIRLSVWGTEVSRVDVLEELQRLGVLHVEMGTEGAPLGDT
                     LRRLKILCATLQGILEALRWDRWELLSDEILDRTSLGLDVGQESTLVGLEKSVEVFRN
                     RLAGLMAERESYEETLRSLKRMQRELAPFENFFREALSKNRMALLFWVPQASAGRMGA
                     MIRDAWTRKGWWNDRDPLRFHRHTQPSTERDELFAVSLPADSEGSVLEELVLHDGGAS
                     WRPTPAFDRDSMAEVLRAMEDGFQWVPRRLSQIQQEIDQARDEWGMRLGALFTLLSEK
                     TEVKTLENALAAQGSLFHLEGWLPEDRQEDTVAALQRRFPKKLFLFWRQAMEDEWHQV
                     PTALRNASLFAPFEIFVNFFSSPRYMGVDPTVLVGIFFPLFAGCIVGDAGYALLLGAV
                     GFFLRRRTAGGAVIRSAGAVALHVAGWSFIWGVVFGEYFGDVGWRLLGLSPLWVDRAH
                     AVMPLLAFSVVLGGGHILIGLGIGCFDALRHRHRHRWMERLGTIFLILGLAFVGAIYA
                     ALIPERAFLGALILLGGGVALILVGGGMGGMVEGVEAVGNVLSYVRIAAIGVSSAILA
                     SVASTFVDLLGVSVLGLFMALSIHALNIVLAVFESGLHAARLHYVEFFSKFFTGGGKE
                     YDPFQRRREVLWRRHS"
     gene            complement(16787..17140)
                     /locus_tag="PL162_00105"
     CDS             complement(16787..17140)
                     /locus_tag="PL162_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90461.1"
                     /translation="MGADNSREIQEILRRELETLRQSLEEELEELRKHEQQEWDRASL
                     RVEEMARATVENMRQEEDREKEALEEERRRLDELVTLRAEEWKAQFLQEAMVLRLAEA
                     MWCRIVPLPPEGMVS"
     gene            complement(17144..17587)
                     /locus_tag="PL162_00110"
     CDS             complement(17144..17587)
                     /locus_tag="PL162_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQF90462.1"
                     /translation="MVSQVMTKDLSAVEEEDSILEAVRIFHSQRISGIPVVRSNWILT
                     GYLSEGDILRAALPTYVEVLMQHSFLDNSEGSLFDRLQALGRRRVKEFMCPDPVSVDT
                     SASLMAVADLMLRKRLKRLPVVEEGRLVGVVDRATFCGFMMDREE"
     gene            complement(17593..19332)
                     /locus_tag="PL162_00115"
     CDS             complement(17593..19332)
                     /locus_tag="PL162_00115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HQF90463.1"
                     /translation="MAIQTFAELLTRSAALLLVLGCYGAQRFLRHHLDRDQYDYVRDL
                     MLCGALALVALWSDSAPAKMVVALSLFAGLLGLAQRRAQVDLRWGYLLIGGLFSLLGP
                     RISFISLPDGEFYYLSILASATATTLWVGLFPLLLQDLDRIPGMAGHLLALVWILTTG
                     LTAISSQELPDALTLSTGALALLAAFWSRHGHMYRQLGLPLASMWGTLIAGTSMLGIS
                     KGVTFSALMLLPLALFAIPLWETSVNVVSRALSADATKTSGGFYQRLVRRGVDHPSSV
                     RAMTLVSAAFGLGAGVLQLRPDPQGFLAAFFTISAALAYLGMVFCRHGRKGQSRRPKL
                     WDVPIDNVSLQYALGKVWSLVKAGKGGTVVTPNALGLYACRRNEALTRAFQGADLILP
                     DGMGLVWGLRFLGRPVQERIAGIDFMDQLCRMAAGAGWGVFFLGGAPGVAEMAAQNLV
                     KRYPGLRVRGAEHGFFADDESEELCRTIRNSGSQLLFVALGVPRQELWMELWRGHLGG
                     VVAMGVGGSFDVFAGTLRRAPECFRNYGLEWLYRLAQEPWRWRRMMTIPLFLGALVKT
                     RLGLDRFRRERDT"
     gene            complement(19579..20853)
                     /gene="serS"
                     /locus_tag="PL162_00120"
     CDS             complement(19579..20853)
                     /gene="serS"
                     /locus_tag="PL162_00120"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HQF90464.1"
                     /translation="MLDIKWVRERPDEVREMLAQRRSAFPLDTLLEVDRRRRDLLGEG
                     EELKARRNEGSRRVGEAKKKGENADALMEEMRLLGERVGAIDEEGASLDRQMEELLSQ
                     IPNRPHVSVPRGADEESNVVVRSWGEPRSFDFEPKPHWELGEALDILDFERGVRLAES
                     RFTVLKGSGARMERALINFMLDLHTREHGYTEFAPPFMVNSATMRGTGQLPKFAEDLY
                     RCAEDDLWLIPTAEVPLTNLHGGEILPEEELPKLYTAYTPCFRKEAGSYGRDVRGILR
                     QHQFSKVELVKLCTPATSYDELEKLTANAERVLQLLELPYRVVCLCTGDMGFASAKTY
                     DLEVWLPSQGKYREISSCSNCEDFQARRMGTRYRPTKGGKAQFIHTLNGSGIAVGRCL
                     IAIMENYQQPDGSVEVPGALRPWMGNQETLGR"
     gene            complement(20856..21335)
                     /gene="ribE"
                     /locus_tag="PL162_00125"
     CDS             complement(20856..21335)
                     /gene="ribE"
                     /locus_tag="PL162_00125"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HQF90465.1"
                     /translation="MRQFEGKFLGNDLRFALVVSRFNELITSRLLEGAKDALLRHGVR
                     HQDLDVYWVPGAWEIPLAVKEIALQGRHDALVALGAVIRGDTPHFDYVASEVSKGLAH
                     IALDQRVPAAFGVLTCDSLDQALARAGSKAGNKGVEAALAALEMANLLREIRTPREG"
     gene            complement(21379..22620)
                     /locus_tag="PL162_00130"
     CDS             complement(21379..22620)
                     /locus_tag="PL162_00130"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HQF90466.1"
                     /translation="MGADEGSVFCSVEEALEELRAGRMIIVVDDEDRENEGDLLAAAE
                     KATPEMVNFMATFGRGLICAPLAPERADALGLAPMASRNTDPHGTAFTVSVDARDGTT
                     TGISAAERAHTVALLASPHAKTESFRMPGHIFPLRARPGGVLRRAGHTEAAVDLTRMA
                     GLAPAGVICEIMNEDGSMARLDDLRSFAATHALRLFSIAQLIQHRTARERLVERVATV
                     QLPTEHGEFVAMAYRSCLEETPERLHLALVKGEVRGKEDVLVRVHSECLTGDVFGSLR
                     CDCGPQLHRALERIDREGCGVVLYMRQEGRGIGLLSKLKAYELQERGLDTVEANQALG
                     FAPDLRDYGLGAQILADLGLRSLRLLTNNPRKVAGLQGYGLRITDRIPLEVDPNPHNE
                     RYLEAKRKKMGHLLHLQDVLG"
     gene            complement(22855..23517)
                     /locus_tag="PL162_00135"
     CDS             complement(22855..23517)
                     /locus_tag="PL162_00135"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HQF90467.1"
                     /translation="MFTGLIESVGTVGGLRTRDDVLLLDVEAPTLAPEVRRGESVAVS
                     GACLTVVEGGSRSFVAEVMPETRRCTRFGRMRPGDRVNLERPLSLGGRLDGHVVTGHV
                     DGLAVVKEVRGRGRTRELFLTAEAAIIDGLVPKGSVALDGVSLTVAEVWGGFFRVGLI
                     PTTLGDTTLGTIAPGAELHVETDLLGKYVERFLARRLGGEGPSTPAPLTWERLAQYGW
                     TP"
     gene            complement(23502..24608)
                     /gene="ribD"
                     /locus_tag="PL162_00140"
     CDS             complement(23502..24608)
                     /gene="ribD"
                     /locus_tag="PL162_00140"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HQF90468.1"
                     /translation="MTRSERWRDERYMRMALSLALRGTGKVSPNPRVGCVLVRENSVV
                     GWGFHGYYGGPHAEVEALRRAGERARGSTAYVTLEPCSHHGKTPPCADALLEAGVGRV
                     VVGVRDPNPKVDGGGMRRLREGGMSCTEGVLEPLCRQINRGFFNVLTRGRPWVTAKGA
                     CSWDGSLALADGQSKWITGERSRRVSHVLRREHDAVLVGIGTVRSDDPALTVREAQGS
                     SPRPVVLDGALCLPEGAAVLNGNRPLIFCGPEAPPERREALQRRGAEVVAVREKVPGA
                     LDLGEVLAALARCGVLSLLVEGGSSVLGSFFQEGLVDGVALFLAPKLLGRGLPFSGDL
                     TIGAMDEAVKLRDPLWTPLGEDLLLEGLISCSLA"
     gene            complement(24738..26309)
                     /locus_tag="PL162_00145"
     CDS             complement(24738..26309)
                     /locus_tag="PL162_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HQF90469.1"
                     /translation="MGNKLSPEALREKIRGLPDRPGVYLMHDDQGEVLYVGKAKSLKK
                     RVGSYFRRQDYASPRLRRLVASIADISVIRTESEVEALIVEARLIKKYQPFFNVELKM
                     GERYPFVKITKERFPRILITRHREEDGGIYFGPFTRVAEVRQLLRLSERYFPLRQCAR
                     EIDPGAPEARPCVRHALGRCLGPCAGLCSEGAYAERVADLVLLLSGKTADLVERLRRR
                     MDRAAQEMAFEEAAHLRDMIRGLWHYTRQRTSLAGHNTLDGETWQVLRQLQELLGLKT
                     LPWRIDGFDISHFSGKETYGVAVVFEQGAPNPSLYRRFVVKSLDGAVDDFRAMEEVVA
                     RRYRRVVQGQEPAPQLILIDGGPQQLAFALDALRGAGLGDIPMVALAERNEEIYRPDE
                     AVPMRLERSDRALHLLQRVRDEAHRFAVSSHRRGRDVRLRRSALEDIPGVGKKRAAAL
                     LGALGSPARIAATDSADLARIPGIGPGLAKRILEGLWRAMDLPRNEGKEDKGSPPSGE
                     GLPSGGGEDGKEGPA"
     gene            complement(26326..27921)
                     /locus_tag="PL162_00150"
     CDS             complement(26326..27921)
                     /locus_tag="PL162_00150"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQF90470.1"
                     /translation="MAMRESEASAVLLRGGRIWTGDPRVPRAEALLWYRDTLEAVGSE
                     EEVRSHPLASRARIISLGGESVIPGLTDAHAHLAFYAKQRHAVPLDRCSSLEDVKRLL
                     RERSAAVPPDSWICGVRFNDTQWEEKRLPTRQDLDALGVPNPVLLSRICCHVQVANSR
                     GLAAAGFPPDAVPSGILYEGEGDPLIQAVERDRDPKECRRALEEACLEFASYGITSVH
                     TCSAGSYGLGDDLAAYQEMRLEGALPLRVVFYTDDMPPAGFLSGLGDDWVRFGGFKLF
                     LDGSLGGRTAALSRPYADAPTTSGILNHEDHALMATMEEAHRRGFQMEIHAIGDAALD
                     QAIEGLRRIKSLGPGRFSRPHRVNHVMICRPDQVEPLRELGVVVDVQPAFVPGEITMA
                     TSRLGEDRLAWAYPWKTFVDAGFVVTGSSDCPVEVPSPWRGIWGAVCRTDDEGCPAGG
                     WTPEQRLSLDEALTLFTVNPPLAVGEGGRRGRLAPGFLADVVVCGEDLFALPQERLRH
                     VIPRATVVGGKVSSGELEGVERL"
     gene            28354..29772
                     /gene="cysS"
                     /locus_tag="PL162_00155"
     CDS             28354..29772
                     /gene="cysS"
                     /locus_tag="PL162_00155"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HQF90471.1"
                     /translation="MALTLFNDLTKRKEPFVPLQPGKVTFYVCGPTVYDFFHIGNARP
                     FIVFDALRRHMEQSGYEVTYVQNFTDIDDKMIHRARELGISVSELASRFIQAYEEDAK
                     ALGIRPATVHPKATEHIPEIVTMISEIIRRGHAYAVEGDVYFDVLSFPSYGRLISQSL
                     DELQSGARIDVDPRKKHPLDFALWKAQKPGEPSWESPWGPGRPGWHIECSAMSSKYCG
                     ETLDIHAGGSDLIFPHHENEIAQSEAASGKAFVRYWIHNAFLLIDAEKMSKSLGNFMT
                     AREARRHFSPLAIRFFMLGAHYRSPLNFSEEALKQATAAVERLQSSWRELSEARKSRP
                     EGQPQEELRQALAAARRRFCEALDDDFNTAGALGALFEAVKAVNSALKDNREGDLDAL
                     QQAEELYRLADAILGVFGLDRPEDPAAAEDAAQIDALVERRLLARKNKDFAEADRIRH
                     ELGSRGIVLEDTPGGTRWHRAL"
     gene            complement(29830..30708)
                     /locus_tag="PL162_00160"
     CDS             complement(29830..30708)
                     /locus_tag="PL162_00160"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HQF90472.1"
                     /translation="MTRRKGSVRRRVLGVLGVVLMAGALSGAFVLPADQQGVVLRLGK
                     VVRSVQEAGLYFKIPFMDTLVRYPKRLLEYDAAPVAVVTSDKKNLVFDSIAVFQIHDP
                     DLFYRTIRTVEAAQQRLDDSVYAAVRIVAGKATFDDLIFNTRDQSLVEATVATNNQTK
                     GYGVEVLRVAFKRVSLPEENEDAVYRSMVAERSRVAAQLRAEGRSEAARLRSEAQKKH
                     TEILASAQRKAEEVKGKGDAQAQQLIADVAVEAPDLYLFLKTLDFYKTNLPGTPIILT
                     PDSGVLRLLKGVSSAP"
     gene            complement(30705..31757)
                     /gene="hflK"
                     /locus_tag="PL162_00165"
     CDS             complement(30705..31757)
                     /gene="hflK"
                     /locus_tag="PL162_00165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HQF90473.1"
                     /translation="MVDFGQNENNPFDSLRRKVHIPGGGHWRRWVGALGGLLLFLGGL
                     LAGVYIVPAGSQAVVFRLGRVLSVENQGLHFKIPGMDAVEIVNTERLRRFEFGYRTVL
                     PGEDPQFRDEPVESKMLTSDNKIVEVNWVLQFQISDPVHYVTRMPLGDRATEKLIRDM
                     AESVLRKVLASRTMDEVLTTGKEAIQGQTRQDVQKVLTDLQAGVRVVAVQLQDVNPPA
                     TVQSAFSEVNSARAEKERLQLEAQQFANELIPQAEGEAQRITNEAEAYSFRVVQVAQG
                     EVARIRALAPMYAQNASLVKNTLWLENMKDVWPRLKVAILDAPAASGAQSFLMLDVNG
                     LLKQLETQGKEGGERP"
     gene            31957..32763
                     /locus_tag="PL162_00170"
     CDS             31957..32763
                     /locus_tag="PL162_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HQF90474.1"
                     /translation="MVAIKSLESLRRALRSLGSAGVALSGGVDSALLAVVLGNTPEVR
                     GHALTLASPLLGREEERRARDVALFAGLPHTLLASNELALPEVAQNSLRRCFVCKAHR
                     LALLTQWGTAAGFDWILDGTNADDLQEDRPGLEALRSCPRVRTPLAEGGWNKNDVREL
                     ARTLEIPVWRRASNACLATRLPFDTPLTASTLRRIDEAEFRLRSLIPEEIPLRLRLQK
                     DSARIETGEGGRTALATPAGEELLGQIRGFLEVSNVFVDGQGYRFGGARR"
     gene            complement(32954..34330)
                     /locus_tag="PL162_00175"
     CDS             complement(32954..34330)
                     /locus_tag="PL162_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HQF90475.1"
                     /translation="MARKQIVYGLLDRPPGPILLLAGAQHVLTLFGATTLVPLIFGPA
                     MGMDVAQVAKFISCVYLAMGIATLIQTTPGLGTGLPIVQGSSFSFIPPIMTIIGIYKA
                     LGPDATMQHVGGALIVGGIALALVGYSGLAGMIRRIITPVVIGPTIMAIGFSLFDVAV
                     KSNAARYWPVSLMVVGGVFFFGLMSKNKYFNIFSVLLSILLAYLVCYLGSAGGFFPEG
                     HPAHVSLVNVVEAPGFRFTGLMPWGPPQFSIVAVGALLAGFFSVMIESIGDYHSCSYV
                     SGLPDPDEKIISRGIGAEGANCALAGLLGAVGTTSYTENIGLIGLTGVASRWVVRAGA
                     VILIAMSMLGKLSALIATIPSPVIGGAYIALFGTIGALGIQVLMRADMGSQRNVLIVG
                     FAFLMALGLPGWVAENQELFFGFGVVGQVVWAILKTPMAVAGLCAAFWDSLVPGTPEE
                     RGIGERSA"
     gene            34825..36798
                     /locus_tag="PL162_00180"
     CDS             34825..36798
                     /locus_tag="PL162_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HQF90476.1"
                     /translation="MIRLTNITLRFGERLLFENLSFFASDSARIALVGANGEGKTTLF
                     RVILKEQIPDAGSVELSRCQNIGHLPQDVAELGSGTVLDFLKERAGVAALEEDLRSLE
                     HRIAATATADGEYRRLLSMHENVAALFLLKDGYSFEAKAKKVLRGLGFRPNAAETPCA
                     VFSGGWKMRIALAAILLARPPVLLLDEPTNHLDTESMEWLEGYLKDYDGCLITISHDR
                     RFLDKMTTQTAELSRGTITLYRGNYTWYLAEREKRREQLIKEMELQRSEIKKTQEFIE
                     RFRYKASKASQVQSRIKSLERFQEVRLESDHKTVKFHFPPCPRSGRTVLTAQDLGMAY
                     GGKEVFRSLNLVLERGDRVALVGVNGAGKSTLCRLLSGEEPPQEGDVHLGFDVKRAFF
                     SQESPRNLNYQNTVWQEVQGLGTRHDDLAKRNLLGAFLFSGDDIHKPVAVLSGGEKAR
                     LGLLKLLLEDTNFLILDEPTNHLDMATKDMFQQALLSYGGTLLLVSHDRAFLDDLVHR
                     VVEIRDGKLFEYRGNYSFFLEKRAENLLAEGAEGSEDPPSFPIASPEKDFSGRKSREQ
                     KREDAAYRARLSVLRRAVIQALDPVEKRIAALEGEKKSLERSLCDPATLGNSGAVQEA
                     LQRLGTIEKELNNLMPRWEELMAEMEAVTQESP"
     gene            complement(36873..37469)
                     /locus_tag="PL162_00185"
     CDS             complement(36873..37469)
                     /locus_tag="PL162_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90477.1"
                     /translation="MEWARQWIGRSARPRALWRRCPLGREGEGWVAMRSPGEPIPLHI
                     VGEPEGWEGGESAIFLALTLGALEGVDGGAPEGDLLRRYALAVVGGRWLEAAGRRAWE
                     RIGADLRAQGETLTLPLSPGEGPSWPLEGQRALGDLLDLGSVGMGLNGHQVLFPLMSL
                     TAAAVVIPGEGTKEGSEGRWDKCLRCALKGRCEWRSGK"
     gene            complement(37588..39432)
                     /locus_tag="PL162_00190"
     CDS             complement(37588..39432)
                     /locus_tag="PL162_00190"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HQF90478.1"
                     /translation="MASKAKDGQEGTPGAFEGAEGSVTIPAGYEGHTVWHALRSLGVA
                     FEAPCGGQGRCGGCRVRLSGGVSPPEEGEKELLGEEGRSQGYRLACRARLTGGARVNL
                     TVGPGKSGGEKGSSGGQRTHWRAPELLIPWDELGEAGSLSEALVRALAHQGEEVGHVS
                     RAALLQASRRWRPGEPGAFILPVRDGVLRGCLSPGRTPLGVACDLGTTTVAVYLADLL
                     TGRVLWSGASSNPQAPFGADVVSRMGAALEGLGPFDELCVALRRAVESLVKEGLASIG
                     GDGEEVLEMVVVGNPPMEHFFLGYLPGTMAREPFVCVDPLPEALSAGCLGMEFIRDAT
                     VRPLPPVGAFVGADLVGVLLAVETLPPKRTRLVVDFGTNGEIALVSEEDLWVTSAAAG
                     PAFEGGHIYQGMSARPGAVRRVRLHDGDLRAETVDSAPPRGICGSGLIDAVAALRRAG
                     VLDDGGRLRGDPFPGDGGDRRRMRGEERGIMLASGDEGEVTIWQRDIREVQLAKAALQ
                     VGICRLLAEGGVKAEAVEECVFAGAFGAELDPEAVRELGVVPPGFDGSFVALEDAAGG
                     GALRVLLEGAPAERRARDVAFRAKVVSLAETPEFQSLFLGAMAFPRRE"
     gene            39617..40222
                     /gene="yedF"
                     /locus_tag="PL162_00195"
     CDS             39617..40222
                     /gene="yedF"
                     /locus_tag="PL162_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HQF90479.1"
                     /translation="MILIDARGKPCPQPVMMAKAAVDQGALEIEVHVDNDISAQNVLR
                     FLGSQGFTSYRSGAATDIVVSGHREGGSTGSQRAPEVPIPVSRSPEGIGVLFLGRTLG
                     QESPELGEALIKAFLGTLAASSPPPATVALMNGGIFLALEESSTSEVLREMESRGCRV
                     LVCGTCVTHFGVADRLAAGTLSNMFEITDAMLRARKTLVLG"
     gene            40234..41403
                     /locus_tag="PL162_00200"
     CDS             40234..41403
                     /locus_tag="PL162_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQF90480.1"
                     /translation="MYFNNAATTWPKPPAVAEKMRDFLTRGGANLGRGSISSRDRATL
                     DLVSTCRERLATMLEGHEGRDPRYVTFTDNVTASLNVVFKGFLRPGMRVLTTSMEHNA
                     VVRPLRSLEESVGIEVVILPCDPRGHLDPGDLEAALASGGDLVALSHASNVCGTIQDM
                     DAIASVCHQRRVPLVVDVAQTAGVLPVSAERWHAAAVCFTGHKGLLGPQGIGGIVWHP
                     EFAHRTRPLVEGGTGSFSHEERQPSVLPDKFEAGTPNLPGIAGLEGALAWIESQGREA
                     IARREHLLGERLLKGLRAIPRATLYGMASMEGRLPVFPLNVEGWDNGTLAFELSERFG
                     LEGRPGLHCAPWAHRTLGSFPQGALRLSPGYFTTEDEVDRCLEALAVLSREPSPV"
     gene            complement(41425..42825)
                     /locus_tag="PL162_00205"
     CDS             complement(41425..42825)
                     /locus_tag="PL162_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQF90481.1"
                     /translation="MRRHLLLALTVAILLPSLAILVASGVSLIEHERAIRVVARSYVQ
                     DLAEMVASRLDLRWGRFGLPGQEYPDRRTLDSSREFLFRFFSWRLSMPGWVAVIDEGG
                     QIMTASPGAESLAQIAPGGVPVGHVLDIKDIHGKKYTIAAYPVRDTRWFVVAAVSWEA
                     LLGPMVRYGSLWALVISGFALAGLAAVWALWRWLIAPLRALENEVSRLRWGEETPAAK
                     GAPAVFELQRLRGVLRHLAQTAIEKSRLMRRSVTDLVRVQEQERTRLAREIHDGPLQS
                     ITALIQQLRLADLAPSIEDRSRRMTMAEEGAHLAVRELRGLCDELSPPWLDLGLSQAV
                     TELAERLSAHLDVAISVECESAADDLSSEAVLSLFRVAQEAIHNAVRHGRARRVELRG
                     TREGNFFALEIEDDGEGFSPGEDFNALRVRGHRGLANMQERMALVGGTLELRSSPGKG
                     TLVRCIVPLGAEGEAD"
     gene            complement(42822..43451)
                     /locus_tag="PL162_00210"
     CDS             complement(42822..43451)
                     /locus_tag="PL162_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQF90482.1"
                     /translation="MIRVFLADDHPLTRSGIAAYLEQEKDMELVGEASDGEEALERIR
                     ALAPDVALMDIRMPKGDGVSVVRRMKEEGLRTAALMLTSYDAQQYVVASLRNGAKGYV
                     LKTATPEELASAIRVVSVGGVYLDREVSRNIEGDFSPEELSVREREVLLLAAKGLSSK
                     EAARELFISDRTVQTHLASIYDKLGAKNKTEAMLLALKYGIVTLEDLLD"
     gene            complement(43681..44676)
                     /gene="argF"
                     /locus_tag="PL162_00215"
     CDS             complement(43681..44676)
                     /gene="argF"
                     /locus_tag="PL162_00215"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HQF90483.1"
                     /translation="MPVNLKGRSFLTLMDFTPEEVRYMLDLSHDLKAKKRTGVREPLL
                     AGRNIVLLFEKTSTRTRCAFEVAAMDEGAHVTFLDSSSSQMGKKESLEDTAKVLGRFY
                     DGIEYRGYQQEVVELLAKYAGVPVWNGLTDIDHPTQILADFMTIEEHVAKPLNKVKLV
                     FAGDTRNNMAYALMYGAAKTGMHFVGLGPKELSPNAEILAKVREEAKITGAVIEVSDS
                     LESVKGADVIYTDVWASMGEEDKIPERVKLLTDYKVTMEMMEKTGNPDVIFLHCLPAF
                     HNFETKLAKDMKEKGYDIREVTDEVFRSRHSVVFDEAENRMHTIKAVMVATIGRN"
     gene            complement(44777..46015)
                     /locus_tag="PL162_00220"
     CDS             complement(44777..46015)
                     /locus_tag="PL162_00220"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HQF90484.1"
                     /translation="MTSDSRGFSVGSEVGRLRRVMLHRPGLELDRLTIHNKDELLFDD
                     ILWLEEAQREHDAFADLLRDNGVEVVYFRNCLAHILREEPLRDELLDDALALEALDIP
                     LAGALKKELMGMNPADLAEVLISGLTKKETEAIVGDIRSLAIRFPDVNDFLIRPLPNL
                     YFQRDPFAFVKNGVIVSCMNFDARRREPLYAHAIFRHHPYFADVQIIFGDDPRDCAPY
                     TIEGGDVLVLSEDTVAIGVSQRTRAGTVQIVGRRMAQKLGIRTVLAVDIPKIRASMHL
                     DTVFTMVDRDAFTIYPGLEEHLHIAELRYDADGALEGIVDHHSLSDCLRRTLHMDHPR
                     FIETGGGDPIAAAREQWNDGTNTLAIAPGVVVTYRQNVVSNQVLRENGVKVFEIRGAE
                     LGRGRGGPRCMTHPLMREQL"
     gene            complement(46165..47013)
                     /gene="speE"
                     /locus_tag="PL162_00225"
     CDS             complement(46165..47013)
                     /gene="speE"
                     /locus_tag="PL162_00225"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HQF90485.1"
                     /translation="MQVMERKTNDLWFTEEQTPDMRMGLRIKQVLRHEETPYQRLLVV
                     ETEEWGRALVLDGAIQITEKDEFTYHEMMAHVPLCAHPAPCRVLVVGGGDGGVVREVA
                     RHGAVSQIFLVDIDAEVTRASREFFPSVSCALDDPRVTVLHEDALVFVKDHQASFDVI
                     IVDSTDPVDFAAGLFCAPFYRDVYQALKEDGMLMAQTESPFAEPRMVTPTFQQMRSVF
                     PLVHLCWGSMPTYPTGTWTYMVGSRRHDPRILRSPVPQGTRYYSEAIHGASFVLPPYM
                     QEQIGE"
     gene            complement(47125..47658)
                     /locus_tag="PL162_00230"
     CDS             complement(47125..47658)
                     /locus_tag="PL162_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90486.1"
                     /translation="MDSEKSQTFPSKTPDLEGVASEETGLRRPWFVLVTGKDLEAFPE
                     APRYDGFAVREALLPAGASTLEGMAWTEGPPPVQSLAAAIVAPPLSESVVGAAVGVLC
                     LADGSRRAAVVTCLGGAEEALMRVEEILFREVDEKDDLAVETLYLAGEEAEAREEEAA
                     TVGAACFFGGLGNEGAS"
     gene            complement(47693..48793)
                     /gene="ispG"
                     /locus_tag="PL162_00235"
     CDS             complement(47693..48793)
                     /gene="ispG"
                     /locus_tag="PL162_00235"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HQF90487.1"
                     /translation="MACARAVCVGALGIGGDNPVRVESMLKTPLEDLAGCEGELRSLD
                     RARCELVRVAFPRRELAGALGELCRKSPMPLMADIHFDPALAVEALRQGCPAVRLNPG
                     NMSQRDLREVVVAAEDRGAALRIGANGGSLSNDQMARAEGDRALALLHAAEDQLAALE
                     REGFSRIILSAKATSVPETVRANVLLGQRHPDYPLHVGITEAGGDVEGIVRGACGIGL
                     LLAQGLGDTLRVSLTGPSPDEIRVGYALLRALELRALGAQLLSCPTCGRRRADVSAIA
                     AALRPHLDSFPDGVTIAVMGCEVNGPREAREADVGVAGSPGGVILFRRGEFLGELPRE
                     NPEDVVEALRRALADPSRDSTPEEGGAPGDGA"
     gene            complement(48862..49890)
                     /locus_tag="PL162_00240"
     CDS             complement(48862..49890)
                     /locus_tag="PL162_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HQF90488.1"
                     /translation="MTFLAFFLVIAVCVLIHEFGHFASARLIGVRVHEFAFGMGPILL
                     SRERGCTRWSFRAFPVGGFVRLAGMGEEQEGESCADEESFGHKRPWERCVILASGAGL
                     NILLAVGLTAALLMGHGVLDLESTVVGEVMAGYPADQWGVVSGDRILSVNGEAVTTWD
                     DMSRRLREKGAQGAVMLEVEHRGAVRVFSGELSRDARQGTPLFGVRPRWVTYGFAAAL
                     GHSFSYTWQMSREILSGMVAWIFARRDIDVTGPVGIANLAGEAARQGMWSFLSFLAVI
                     NLHLGLLNLFPFPALDGGRLFFVFGEMITGRRLPERWEQYIHLGGFAVLMALILVITW
                     QDLVKLLG"
     gene            complement(49887..51065)
                     /gene="dxr"
                     /locus_tag="PL162_00245"
     CDS             complement(49887..51065)
                     /gene="dxr"
                     /locus_tag="PL162_00245"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HQF90489.1"
                     /translation="MTRTPKDVGIIGATGSVGGAALWICEKFPDRFRVRALAAQRNVA
                     AMESLVRRFNPEVVVLAEEGAAAELASRLGGEGRVFGGAGALDRMVAELPGVHWVFAS
                     SGTGALGALERALVQGDDVSLANKESVVVAGPWILPLVSRKDQLRPVDSEHNAIWQCL
                     FGEKEEDVDRIWLTASGGPFRNFSREEMARATPEQALRHPTWAMGAKITVDSATLMNK
                     GIEVLEAMQLFNLPVHRVHALVHPGSLVHGVVRFRDGCSKMVLSTPDMRIPAGVAMAF
                     PERLPIMETELAPPPPTPMNLALDAPDEGRFPCFSLALEAGRRGGAYPALLVGADEAA
                     VRAFLEGRIGFLDVASVVEETLEGWKGAAPASLGDALEAVEEGQRRAEAMMGEGRFRR
                     "
     gene            complement(51062..52015)
                     /locus_tag="PL162_00250"
     CDS             complement(51062..52015)
                     /locus_tag="PL162_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HQF90490.1"
                     /translation="MDRDPMISEEKEAPSVETRASQDDSRDPGISAGTKKLPRSPLPS
                     PKRGVGGRVLLVRALSGGLVVGAVVGGLLTGGYGWIAVVSLLALISLGEFYGILRRRF
                     HVSSGIGLLGGLGILAASVFEVPERVDLLILTLVALGTLGIEVFRRQLTGTSQAIFNM
                     GGTVGGVIYVALPWSFLLDMRTEPWGQMVLFSLFLCTWSCDVLAYLVGLVWGRTPLCP
                     DISPKKTWEGALGGLLGALGASGVVAYALAAPPMPLLIIGLICGTWGQVGDLAESALK
                     REALVKDSGKILPGHGGLLDRFDSLLFSVSAVYLLFGVIWS"
     gene            complement(52008..52733)
                     /gene="uppS"
                     /locus_tag="PL162_00255"
     CDS             complement(52008..52733)
                     /gene="uppS"
                     /locus_tag="PL162_00255"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HQF90491.1"
                     /translation="MWQEESAAGGARHVAIIMDGNGRWAKARHLPRIMGHHAGVQAVE
                     RTVRAAKDLRVPVISLYAFSTENWKRPKAEVLGLMGLFRYYVAMKVKALCEEGARLRF
                     AGSREGLPEDVIRTLEDAENATASCRAIDVVVCLNYGGRREILDAVNALLASGHSGSV
                     TEELLRSQFYIPDLPDPDLVIRTSGEHRMSNFWLWEGAYSELYFTDTLWPDFGREELE
                     KALKIFSGRERRYGGVGGSEKNG"
     gene            complement(52733..53392)
                     /locus_tag="PL162_00260"
     CDS             complement(52733..53392)
                     /locus_tag="PL162_00260"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HQF90492.1"
                     /translation="MDLLYDPEGLEVAKERGRMWEALEREAESCARCPLGAGRHRVVL
                     GDGNRRSSLLLVGEGPGAEEDAQGIPFVGAAGQLLTKILEAAGIGRQEVYITNIVKCR
                     PPNNRAPQMEEMLACDRFLQAQILLLNPRILVCLGSTPAKWILKTSEGISKIRGKWFS
                     WRGIDVMPMFHPSYLLRNQSSTVGSPKHQTWLDIQEVRKRWDAWGPRGSLSEIPKGGM
                     S"
     gene            complement(53392..54219)
                     /locus_tag="PL162_00265"
     CDS             complement(53392..54219)
                     /locus_tag="PL162_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90493.1"
                     /translation="MPEDLLDGVEVLAFVGPAGTGKSQRAQLVAGEHDTDFLIDDGLL
                     IHRGQIVCGRSAKTERNQICAIRRALFQFPAHRLQVRRALAASAPCRVMLVATSEEMA
                     RRISRRLGIPPPRAFVHIRDVASPEEIARARNERIHKKQHVIPVSSLQVRKNFAGKMV
                     GRLRILWSGEEPQGEKTIVRPPFTFLGNLAIEPEALAQIIREISQRTPQVASVKEVRV
                     DSQGDRLRFSITIAVSVGEMTMLDLALFLQKRLSSSVAYFTGLEVGKVDVVVSEVVL"
     gene            complement(54223..55338)
                     /gene="hisC"
                     /locus_tag="PL162_00270"
     CDS             complement(54223..55338)
                     /gene="hisC"
                     /locus_tag="PL162_00270"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HQF90494.1"
                     /translation="MSWLERLGRKALMGIEPYRPGKPISDVQRELGLKQVIRLSSNEN
                     PWPLPERLSQVISTAAQDVRRYPDPGAYNLRRALAKKWGVSPSEVVVGAGTEGVLFSL
                     FQATLDEGDEVVYCTPTYPLYRLAACAAGARCVEVPLGDALAPGADQLLSACTPKSKV
                     VVLCNPNNPTGALISRNELLALAGALEGRETLLVVDEAYAEYVVDPRYVSGVELFRQL
                     GNVAILRTFSKIYGLASLRVGYAIVPKLVGESYAKVRRVFDVNRVGQEAAVAALEEEI
                     FVEETRTKTIAERERMATELTTLGVKVIPTHTNFLLVFLADADRVADDLLHDGIIVRP
                     GSDLGMAGALRVSMGLPEENDRFVASLRKQLKRPEKR"
     gene            complement(55531..56814)
                     /gene="murA"
                     /locus_tag="PL162_00275"
     CDS             complement(55531..56814)
                     /gene="murA"
                     /locus_tag="PL162_00275"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HQF90495.1"
                     /translation="MKEGQKIVIQGGKALRGSVRAQGAKNAALPILASALLLSEGTLQ
                     LERVPDLEDTRTMVQILQHLGAEAQFENGAVRVHTPAEIAWETPPQLVRRMRASSLVL
                     GPLLARCGRASLPLPGGCSIGGRPIDFHLKGLAKMGAEIELVHGTVQARAKGLRGCRL
                     YMDFPSVGATENLMMAAVCARGETVIENAAREPEITNLAQALRSMGAIISGEGTGTIH
                     VKGDTALHGATVRTIADRIEACTYLLAGIITGGEVVVEDVAPEHLEAFFAKLEEAGAE
                     AWASGGDARARRSARLSSLSLKALPYPGFPTDLQPQMMAVLALADGTSIVQDGVFESR
                     FLHVNELQKMGARIDVQGGTAVITGVPHLRGADVRATDLRAGAALILAGLAASEETAV
                     FQIQHINRGYENIVTKLRSLGAAINLDSSEEEDLA"
     gene            complement(56811..57959)
                     /gene="wecB"
                     /locus_tag="PL162_00280"
     CDS             complement(56811..57959)
                     /gene="wecB"
                     /locus_tag="PL162_00280"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HQF90496.1"
                     /translation="MSTEPRTVILVVGTRPEAIKMAPVFRALKKRSEVRPYLLATGQH
                     AEMLLEPLSYFGLVPDENLGIMRERQTLDHVTSAVLGLSGQVFDRERPALVLVHGDTS
                     TTLAAALAAFYRKIPVGHVEAGLRSGDMAHPFPEEMNRVVTDRLASWWFAPTMGAAEN
                     LRREGCRPDRVVVTGNTVIDALLWTMSRAGRPSTPALLDLPPEARVLLLTAHRRESWG
                     AGLESICRAMGRILDRVPDVWGVVPMHRNPAVRETFQNFLGHHPRVRLCDPLPYPDFI
                     WAMGRSTLILSDSGGVQEEAPVLEKPVLVLRSVTERPEALTEGTAVLVGTDEERIFST
                     AYRLLTDPEAYGALARKGMNPFGKGDAAEKICEALLSPMGGLTAGGSR"
     gene            complement(57998..58912)
                     /locus_tag="PL162_00285"
     CDS             complement(57998..58912)
                     /locus_tag="PL162_00285"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HQF90497.1"
                     /translation="MWRVVFAALSGLLWGLCITPLTMRLAVHYRVVDHPGGRKRHSQV
                     MPRGGGLALWSGCVLWSLFAQTGDRDLPLLLTGATIVFLVGYVDDMRPLSPALRLVFH
                     LIAAGLAVLPLDGPWELRALFLLWIAGCTSAFNNIDGLNGLCLSLAGCCFAVPLILGG
                     RGMWAALWGLCAGVLWWNFPRARTFLGDGGSNLLGFLWGALWVQFRGDFMAQRPLLSL
                     MAVLFLLGGVPILDTAYAILRRSLKGKSPFAPDRGHLHYRLLDGGVPMGGVVLLLCAL
                     HVALLGGGIYLLYGTLRLSMLAPFPGGI"
     gene            complement(58922..60028)
                     /gene="upp"
                     /locus_tag="PL162_00290"
     CDS             complement(58922..60028)
                     /gene="upp"
                     /locus_tag="PL162_00290"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HQF90498.1"
                     /translation="MGIVVGSDHAGFRLKEAVRRHLVEAGHDVEDFGVEQEVPGADYP
                     EIAFRLAEAVALGKAPRGILVCGSGIGMAIAANKVRGVYAALCHSPFEARLSRQHNNA
                     NVLVLGGRILGEGVALETVDVWLGTSFEGNRHLRRFRQIQDYEQRREDAADRASAVTN
                     PRLVVVDHPLIQHKVGIIRDKGTTVKEFRELVQEIAGLMVYEVMRDLPLEDSEVETPL
                     ERTAVKSLAGKKLAVVPVLRAGLGMVDGILRLVPNAKVGHIGLYRDPESLMPVEYYCK
                     LPGDVEDRDIFILDPMLATGGSASAAIALTKRWKARKISLVCLIAAPEGVQKVHQDHP
                     DVDIFTAALDSHLNDHGYIVPGLGDAGDRLFGTK"
     gene            complement(60205..61137)
                     /gene="meaB"
                     /locus_tag="PL162_00295"
     CDS             complement(60205..61137)
                     /gene="meaB"
                     /locus_tag="PL162_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HQF90499.1"
                     /translation="MSGTVERALAGDLRAMARLITLVESESSAADGIMRAIYPHTGRA
                     HIVGITGSPGAGKSTFSDKLIMSLKEQGASVGVVAVDPSSPFTGGAILGDRLRMQRHA
                     LDPSVFIRSMGSRGSLGGLGKATYEAALILDACGKDVVLIETVGVGQSEIDIMKIADT
                     VCLLLVPGMGDDIQIMKAGIMEIADIFVVNKADRDGADRIVNEVNIMLDLLGERPWRP
                     PVLKTVAETGEGVGAMGGALKGHRDFLLRSEAGRRRRWQRLAKEVEDILRREIARVAE
                     HAWDEIRTPELLMDLEERRRDPYDVAENILKDLL"
     gene            complement(61157..62977)
                     /locus_tag="PL162_00300"
     CDS             complement(61157..62977)
                     /locus_tag="PL162_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90500.1"
                     /translation="MLLVLGGVSWGEGLPEAQRGEAERYLFLAYDLLVRQRYWESLDA
                     LEQALARNTYLVDYYLLRGLVLRRLGFLAEATAAVEHYLEVRPREEIPRRIGASLREE
                     RDSVRDLLRGPIGAPSFQFQRSPVEVFFRLPLTMPWNARGIAKARMVGPAQVVADELG
                     NSLTQFFPGASPSMRRISTDAPVAVCPMDLKKFLVISRSGGVWEGSLDSSDLEPRGTL
                     SAIPADGECVASGLLGVADWGRRRVVFFDGPSFREDWSWDPQRADPHHPFEPVAVSSL
                     GPLMAVADRGGNRVYVLDTQKRQLEKELSVPSPRDVLWSRSNALMILSEEGKLYLWTE
                     GEPSLLLEGLKDAWALSESPQGVIIWDVAGNALWSGVEYPPVAGMATMMCLAAPGSVG
                     GENPQAVLRAYVGEPARPLFPRGRSFVSAVWMGRKMEGTIVPRASGEDRGVLLLGGAG
                     RAEETKDLSVAVLQDGSMLLSALRQACKDTGTLPSCLVVDAAIPFSPEDLERLFSMAL
                     NNGIRVYALATQIPSAPLARLVRMTGGRVMPTERLDALPSAPRASWEIRVPLPVDALP
                     SGYPSEAMLSIYVDAGSLTLRDWIPYWPVGFDMGAQPLAP"
     gene            complement(63013..63225)
                     /locus_tag="PL162_00305"
     CDS             complement(63013..63225)
                     /locus_tag="PL162_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliR"
                     /protein_id="HQF90501.1"
                     /translation="MSDINIQALAALFSFLVALGLARLVALVHRGALPGGAPWVAYLR
                     GLVGFFFTGALVLGFYSLAGVSLWRS"
     gene            complement(63378..64346)
                     /locus_tag="PL162_00310"
     CDS             complement(63378..64346)
                     /locus_tag="PL162_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQF90502.1"
                     /translation="MVMIRVGVIGVGHLGYHHARVYTEILGAHLVGVVDINEDRAMGT
                     GESLRVPFYGDLDTFLDREKPDAVSVVVPTCAHYEVTRSLLLRGVHVLVEKPVTSRVD
                     EAEDLLRLAAQKNLLLQVGHIERFNSATQYVSNIVNEPLVLQSRRLGPFSPRISDVGV
                     VLDLMVHDIDIILSLVHSEIVHIAAMGRSVRSDHEDIASAQIQFANGAMADILVSRVS
                     ERRLRQLEIMEPARYINVNYETQDVSIHRCVRDNGTSLMEVVEQPIFPKREPLKLELQ
                     HFVSCVQDGRQPLVGIGDGKRVLEVAVAVLRQIAEGNEGARLRQIG"
     gene            complement(64383..65180)
                     /locus_tag="PL162_00315"
     CDS             complement(64383..65180)
                     /locus_tag="PL162_00315"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HQF90503.1"
                     /translation="MRLLPEATLWPPRISGASPAEVRYHLKGVDDPVAILMGRSDAAE
                     RAKGDPEAHWRALGYDLRRDPLVAPRQVHGVAVVSGRRIWALPQRPQADGVELDVAGV
                     WGSLRFADCWPVILAGTFPTPWVMLLHAGFRGAVAGVLAQNLRRLLDRRGPRAREGLC
                     VWMGPGIGPCCYPRSREDPWVKRGYALFPEAFAPLSEELVTVDIGRILCQQCDDLGVP
                     RESLCRSPWCTACASDYFYSYRRKDLEARNFLVAPGPQKGFWWDNIL"
     gene            complement(65177..66493)
                     /locus_tag="PL162_00320"
     CDS             complement(65177..66493)
                     /locus_tag="PL162_00320"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HQF90504.1"
                     /translation="MGAENPWDFLEDRLTKAASPGIRPGLARISRLLGLLGHPERAVP
                     ALHVVGTNGKGSTCAFLDSILRAQGYRTALYTSPHLESPGQRLTLEGNPLSPQEWEGA
                     AEEIWGALDRDRELRANPPTFFELVTAVTFCLLRTQAPDVVVLEAGLGGRLDATNMAS
                     RVLLSVVTPIAKDHEEFLGSTLEAIAAEKFAVIRPGGRAIFAGEPPELEGAFRERALR
                     GGIEGRVLSSEWRLREEALSPAGGRFSLYGRSRGWEGLEISLLGPHQLRNAALAVAGL
                     DLIGDLLPVTEGALRKGLSLARWPGRGELFPGDPPVLLDGAHNPHGVAALTKTLGRLW
                     GPRAPRTIVFASMKDKDYQGALSQLLAVGSELICTRVTGNARSAAPDDLVCQARHAGW
                     RDVSSVEAPEEALARARAKGRLVVVCGSLYLIGILRPLLTEGGGEP"
     gene            complement(66500..69151)
                     /locus_tag="PL162_00325"
     CDS             complement(66500..69151)
                     /locus_tag="PL162_00325"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HQF90505.1"
                     /translation="MDPRGEIGKAYDPKPIEDRRYAAWMAEDLFRADPHEEGKKPFCI
                     VIPPPNVTGSLHLGHALDNTLQDIVCRWHRMRGDNVLWLPGTDHAGIATQNVVERQLA
                     SEGTSRRDLGREAFVERVWAWKEQYGSRIISQLKKLGASCDWSRERFTMDEGLSRAVR
                     EVFVRLYKKDLIYKGKYIINWCPRCHTALSDLEVDHDDTAGKLYFVAYPFEEGDGALT
                     VATTRPETILGDVAVAVHPRDDRNRSFVGRRVRVPLTGRLVPVIEDNMVDPEFGTGCV
                     KITPAHDPNDFLVGQRHGLEQIQVIDGEGIMNEAAGIYAGKDRFEARKLVVEALGREG
                     LLLKAEEHQHAVGHCYRCKSVVEPFLSEQWFVRTKPLADRGVQAVREGRIRFVPEQWT
                     HTYYQWMENVRDWCISRQLWWGHRIPAWTCAACGHITVASEDPSACERCGASTLAQDE
                     DVLDTWFSSALWPFSTLGWPDKTPDLATYYPTSLLVTGFDIIFFWVSRMIMMGLEFMD
                     DVPFREVYIHALIRDERGQKMSKSKGNVVDPLDIIDEFGADALRFTLAALTGQGRDIL
                     LGRSRIETYRFFMNKLWNASRFALMNLEGAPEGSLDPEDPLRLPDRWILRRSREVAKE
                     TTAHLSGYFYAEAARGLYDFVWSELCDWYLEMAKPALRGEEGEGRRRATQKVLRQVFR
                     TTLLLLHPFIPFVTEELWEAFGFGARPMDQERWPEDPVNGDEESGPEMGLLQEVVRTL
                     RNLRSEARIAPSQTVPSAQVVIPGEGRRQVVEDHKDLACLLAKVDHLVVLPRGDKPAR
                     SLVTIAPDFAAYLEVGSLLDVEAELRRLEGERAKLAKDVELCEAKLRDEKFLSRAPEE
                     VVCKERDRMRDGKLRMARIAENLESLR"
     gene            complement(69294..69935)
                     /gene="yihA"
                     /locus_tag="PL162_00330"
     CDS             complement(69294..69935)
                     /gene="yihA"
                     /locus_tag="PL162_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HQF90506.1"
                     /translation="MGHWHVSLGHTCFTPDQIPPPSLPEIAVAGRSNVGKSTLINALL
                     GTKLAHVSSAPGKTRSINFFRIRVARTDLSFYLVDLPGYGYAKRGHEERRHWGRLIEE
                     YVRDRSPLCLVLQLVDFRHGFLENDLMMQGWMRDLALPGQVVFTKADKIARAHHGGLC
                     RRYAYPGMVSIYPPLVTGAAKRQGVAELGSFLEGVVLARSPRAPEGGAAGEGA"
     gene            complement(69938..72277)
                     /gene="lon"
                     /locus_tag="PL162_00335"
     CDS             complement(69938..72277)
                     /gene="lon"
                     /locus_tag="PL162_00335"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HQF90507.1"
                     /translation="MGDRATLRVPVLPVRDIVLFPGIIVPLFVGRPRSLKALEDAMLK
                     ERKVLVLAQREMITDDPREEDLFRIGTLCQVLQMVRVPDGTTKALVEGMERAAVESFS
                     TEKDYFEAFLSPVLESSGDLERIEALRRGVMEHFERYVALHPKIPNEVAMSVFSVEDP
                     GQFADLVASHLLIKVPQKQSVLEVLCEEDRLALLLKILLEEIEILELEHDIHDRVRQE
                     MERSHREYYLREQLRVIQDELGHGEGMSEVEEYRQTILGAQMPPDVEKRALKELDRFA
                     KMPPMSAEATVVRTYIDWMAAMPWSHESRDRLDIGVAKQVLEEDHYGLEEVKERILEF
                     LAVRKRARSRIKGQVLCFVGPPGVGKTSLGRSVARAMNRKFVNISLGGIRDEAEIRGH
                     RRTYIGALPGRILQKLRSCGTKNPVLLLDEIDKIGADFRGDPAAALLEVLDPEQNATF
                     SDHFLEVPFDLSKVLFLTTANVAHTIPRPLLDRMEVIQIPGYVAEEKHHIARRHLWPK
                     VLEEHGLKESDVRLPDRIIQTVVSDYTREAGVRHLERQLAKVARKATRLLVEAKEKKE
                     KISSPLQVTKGVLRQFLGAPLARDTRLPDEDSEGAAVGLAWTETGGDVLIIEAVIMKG
                     KGAVTFTGNLGSIMQESAQTALGYLRSGASQYGLEKVSWDKTDVHVHVPEGAIPKDGP
                     SAGITLAVAMLSALGRRKVRHDVAMTGEITLRGNVIPVGGIREKVLAAKRQGIFDIIL
                     PAANLADVEQLPAWIRKGLTLRYVHHLSEVVAVALGQSA"
     gene            complement(72277..73560)
                     /gene="clpX"
                     /locus_tag="PL162_00340"
     CDS             complement(72277..73560)
                     /gene="clpX"
                     /locus_tag="PL162_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HQF90508.1"
                     /translation="MFKYEGDNKDGKRRGPHCSFCGKGQKEVQRIIAGSNVYICNECV
                     SLCNLILSSGDGAEGGGMEGASRTREMLKPVELKEFLDQYVIGQDLAKKVLSVAVYNH
                     YKRIASAGELDQDVELQKSNVLLIGPTGSGKTLLAQSLARKLDVPFAMADATTLTEAG
                     YVGEDVENILVRLLQAADYDIQAAEKGIIYLDEVDKIGRKSESPSITRDVSGEGVQQA
                     LLRILEGTVANVPPKGGRKHPHQEFIQINTSNILFVCGGAFAGLEDIVARRMNRKLIG
                     FGGDIVGSGKTETYQLLRETLPEDLVSYGFIPEFVGRLPVLAALEPLSEEALVRILVE
                     PRNALVRQYRKVFEMEGIDLEFTPEALRSISAQAFKRNTGARGLRTIMESLMLDLMYT
                     IPSSKEGISRIVINDRTVEEKDHPALFSGDGKEVA"
     gene            complement(73783..74364)
                     /gene="clpP"
                     /locus_tag="PL162_00345"
     CDS             complement(73783..74364)
                     /gene="clpP"
                     /locus_tag="PL162_00345"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HQF90509.1"
                     /translation="MPLIPYVIEQTGRGERSYDIYSRLLKDRIVFLGDGIDDHVANLV
                     VAQLLFLESEDPDKDISLYINSPGGVISSGMAIYDTMQYVKCPVSTICVGQAASMAAV
                     LLAAGAPGKRFALPYARIMIHQPSGGSQGQAVDIEIQAKEILRLKDILNRILVQHTGQ
                     PMKRVAADTDRDFFMSAEEAVRYGMVDRVIDRR"
     gene            complement(74364..75728)
                     /gene="tig"
                     /locus_tag="PL162_00350"
     CDS             complement(74364..75728)
                     /gene="tig"
                     /locus_tag="PL162_00350"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HQF90510.1"
                     /translation="MRSEIVSQENTTVVLHVEVEQERFDGAVRDAVREMSRNVELKGF
                     RKGHVPRRALDMYFGSRAIVNEALEELMPRVVNELAEEYDLAPIESPSFNFISAEEGK
                     PLVVDITLEVRPEVTLPPLEEIEVDKPLIDVAEDDVEKAMDEFRRVRATMASVEGRPA
                     GEGDTVMVSYTATVEGEEDPVETNTSPIALGAPQLRKEIRDVLMGREKGDEVDVMVSM
                     EEDANPRYAGRNVRYHFVVTDIQEEILPELTDETVPQLTSGAAKTVEELRRRMRRRLE
                     SEVQSRVDMMVRNEAFDQLVAKASVASLPEKSVLRHMNQLRQDDLEELRRQGKEDLEE
                     VLRAQGKDLAAYDQGLRERGEALTRNGFVIDVLADQNQIEVSQEDLEMEFRRVALRTG
                     TPLKKVYEQYTQRRDLLGELMAALRTRKTLDHLVSKVAVREMPMEEYRGKHGGFDASG
                     EGNA"
     gene            complement(75978..76889)
                     /locus_tag="PL162_00355"
     CDS             complement(75978..76889)
                     /locus_tag="PL162_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQF90511.1"
                     /translation="MAMGGVLAVLCCTFIWGVMGPIMRQGASYGLTSFDISLVRATGA
                     ALLLGGIGFLRGKSLRCPRPLILYRAVNGLFGVVGLYVLSNYALITIPVGLATVLFYT
                     APLWVVVLAHVLGKEPLTPLRIVSVGMALGGVWWSAGEIHFLGYGLWGPVAMLVAGFS
                     YAVFLLNGRYAPGADDTFGTYFHTYVAGTAVMWGLACFTSTLSHLRALPWEGWALLAY
                     LVLIPTLGAYGLVMWALRRVPSAVVSLLSMTEMLFAIFWGWWLLGEIPTPATLGGAAF
                     IGTAVVLVTLEGWVAWGKTPLVEDPKP"
     gene            77233..77901
                     /locus_tag="PL162_00360"
     CDS             77233..77901
                     /locus_tag="PL162_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5752 family protein"
                     /protein_id="HQF90512.1"
                     /translation="MTPHPFIQQGCALVTMSTGLRAQSLKELRQGVSLASDQCLHYHF
                     WGRLLRPQFGEADFANDFAAWVAGPVGDPATAERLNILNPSHVPSFEDLRRDILDILD
                     DRLSREDFLSWARADRDFFFTDAAMIVFDTELRAHSPAELASLVENASQGSLFFHFVD
                     ARRRTPDHHDDFSSWLVHACEDPEDSPRIRALRALDPFLLRVTELRQRVGAALRIGEE
                     EAPA"
     gene            77898..79139
                     /locus_tag="PL162_00365"
     CDS             77898..79139
                     /locus_tag="PL162_00365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQF90513.1"
                     /translation="MKTLLASYREVVGSEEIAVLEQLASRLQGRRVVHVNSTREGGGV
                     AEILQRLIPLQQELGLEARWETVDGPPEFFQATKTIHNTLQGAKGNLAARYRDAFEGA
                     GLRNSEKLKNILEGADFVFIHDPQPAPLIQHCSDRRGCWIWRCHIDLSSPSRTVWRYL
                     QPWVASYDASIFSLPDFAQPLPHPVFLIPPCIDPLSDKNILLPEEEVLSVFDRFGLDR
                     ALPLVLQVSRFDHFKDPLGVIEACRIVMKTAPMQLVLAGGGASDDPEGEAVLQEVRRA
                     AEGIPHVHILVLPSDAHRTINALQRAAAVIVQKSIKEGFGLTVTEAMWKGKPVIGGNV
                     GGIRLQIIQGQTGYRVHTPEGLAFRLRHLLQRPEEGQVMGKKGREYALENFLLPRLVR
                     NELAIMLLLLQETPHRLEVTV"
     gene            79140..79931
                     /gene="otsB"
                     /locus_tag="PL162_00370"
     CDS             79140..79931
                     /gene="otsB"
                     /locus_tag="PL162_00370"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HQF90514.1"
                     /translation="MISAALSVAGALRASLLRASRRVLGLDFDGTLAPFRPRRMEVTP
                     YPGVVPMLEKIHAQGDLLAFFSGRPVAEVRHLLKPLGDRCPIFGCHGGEEGWPGEEPF
                     LLPLPEQTQEGLAVAEAMFRERWKHKGFTLPCPLERKHGTLALHTRNYRLPEQDFSVE
                     IWASVARETGLQILPFDGGWELRHRAFNKGKALERYLASLPPPEPPSDFVAYLGDDLT
                     DEDAFAFLARRGLGILVRSRWRPTQASAWCVPPEEVLQFLSLWEK"
     gene            79952..>80335
                     /locus_tag="PL162_00375"
     CDS             79952..>80335
                     /locus_tag="PL162_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HQF90515.1"
                     /translation="MTENRYERLVVVSNRLPVVLHRQEDSWQIKGGSGGLVSALGPVL
                     RDRGGVWIGWPGTSETIGIREVRNILGPASESSGYRLFPVFLSPEEVESFYQGFSNGI
                     IWPLFHDLQGKCEFLPDHWQAYLRVN"
BASE COUNT        15585 a        27654 c        22719 g        14377 t
ORIGIN      
        1 cttggcatgg ttcatggctt ccacggtttg gggcttaggc cgtcgtccgc cgccaccacg
       61 agaatggcga tgtccgtcac ctgggccccc cgggccctca tggcggtaaa ggcttcgtgg
      121 ccaggggtat cgagaaagac gatcttctcc ccgttgaact ccaccaccga agcccctatg
      181 tgctgagtaa tgccccccgc ttcgctagcg ctgacgttgg tctttcggat gcagtccaac
      241 agagtggtct tcccgtggtc cacgtgaccc atcaccgtca cgatgggagg gcgctgttga
      301 acgtgttttc cctcgtcggc tttggacgtt cgggaagagg tttttttctc ctccgtgctc
      361 tcctccgggg ccaccgccgc ccaccccagg gaaactccga aggccttccc cagcgcatcc
      421 agggccctct catccacctt ggcattggca gggatcatca ttcccgccgt caccagagct
      481 tttaccgctt ctcccggagt gacccggagc agggacgcca cctccgccac ggtggattcc
      541 agggggacga ccaaggattg ccccgaggct ggggcctcac tcaccactgg agcccccgac
      601 cgtttaagaa aatcctccgc cttctgcccc aattcggtat cgatggaact catgtgggaa
      661 ctgacttcaa tctcaagctg ctttagtact cccatcaact ctttattcga cactcccagg
      721 attttggcga gctcgtacac acgaatcttg ctactcattc ccttcgcccc cctttgacaa
      781 aagcgactcc acacgttgcg ccaggccgtg atctcgagga agccccacga tctgggatcc
      841 tggaagcccc gtacggccct cgatcatctc ccgactagcg cctagcacaa acaccgcgca
      901 ccgccctcgt tcctcgtatc cccggagcat gcggcgcaca ttcccggaag catcggaagc
      961 caaaagaagc gtcatgttct tgccgcaacg cagcgcctgg cgtatcccat cctggccaag
     1021 gacaagcacc cccgcccgcc gggccaacat gaccaagggg aagagttcgt ctaaaacatc
     1081 catgaaaaat cacacttctc cttcgatggt cccgccgaca caaagcgctt ccaacgccaa
     1141 atagatttcc tctggaacct tgactttcag cgacctggaa agggcgtcct tctttcgggc
     1201 cgcctccaag cacggccttc gcctacagag gtaaacgcct cgtcccgagg cttttcccgt
     1261 gcggtccacg gccaccaccc caccggggct ccggacaatc cgcaccaaag cagaccgagg
     1321 ggcctcgcca ccacatccta cgcacgttcg aggtcgacgc cgctttattt ctcccggtcc
     1381 tcccatagtt ccttcggcgc ccccttgatc aggtcttcga aaaggtcttg caacgtgggc
     1441 atcctctccg gctcaaggac tttgatgtcg atcttccacc ccgtcaaccg ggctgccaag
     1501 cggacattct gccccgcctt accgatggct agggaaagtt ggtcggggcg aacaaagacc
     1561 ctcgccgccc ggtcagcctc cagggagggc tccacgcgca ccaccttcgc gggggatagg
     1621 gcattgcgga tgtactgcag aggatcggcg ctccagatga tgatgtccat gcgctcgtcc
     1681 ccgagctctc cgcccacggc cttgatgcga gctcccttgg gccccacgca ggcccccacg
     1741 gggtccacgt tcgcgtccat ggaaagcacc gccaccttag accgggtacc agattcccgc
     1801 acgatgttac ggatctccac cacaccctcg gcgatctccg gcacctccac ctcgagaaga
     1861 cgcctcaaaa gtcccggatg cgtccgggaa accacaatgc ggggcccccg ggtggtacgc
     1921 cgcacgtcca ggaggaaaaa tttaaacctc tcccccgggg gatagctttc cccgacgatg
     1981 cgctcctccc gagggagaat ggcctccgtc ctctcgttta gccggactag cacgtgctct
     2041 ccttcggttt tgaagatgac ccccgtcacc aaatcgccga tcttatcggc gaattcgtcg
     2101 aaaatcacct gccgctccgc atccttgagg cgctggatta ttacctgacg ggccgtctgg
     2161 gcggcaatgc gaccgaatcc ctccggaaat acttcgatgc gaacgacgtc gccgggatcc
     2221 acgtcatggt atcccaacaa ccgggcttcc gtcaaagaca tttcggtgtc gggatcggac
     2281 ccctctgcca ccacccgtcg gacctcaaag agggagattt cttctcgttc caagtcgagg
     2341 ttcacttcca cgttctggtt gcccccccgg tgcttccggt aggccgaaat caacgccgct
     2401 tccaggctgg aggtaatgac ccccacggga agaccttttt cttcctcaat ctgatgtaac
     2461 gccttgacaa aatcacgacc tagctgcacg cgcggtccct cctttaccgg gtttggaagg
     2521 cttttccggc tcgtacacca gatgggctcg gcccatatcg gccagggaaa tcacgacctc
     2581 gacgccgtca tcccggcgca acgacaccgc atcttccgtc acatgactca gggttcccgt
     2641 gcacttcact attttacccg ccccatcccg atgactcaac tgagccctct ggcccagaaa
     2701 tctttcaaag tccttctgcg aaaagagggg gcgctccacg ccgggggaac tcacttcgag
     2761 gaaatagcgc ccttcaaaga gatcttccgg cccgtcgaac agggggctta cctttctcga
     2821 gaccgtctcg caatccttga cgttgattcc tcccaaggag tcgatataaa ggcgcaccac
     2881 tgattgatcc ccttccacga ccctctccat gcccacaaat tcgtacccca ggccctccac
     2941 cgcttcccga acgaggtccg tcggaagagg ggggcgcctt ttgtgtccga ccactcgtca
     3001 cacctccacg ccaacgcatt ttctcattgc cacaatgatt gattgaagaa ccatcgcaag
     3061 tttccctgta cacggaacaa agccccacaa aaaacgaaga gtgggtccgt cgcccactct
     3121 ccgccctacc atccaaaaat ccgccccacc gtgacggtca gtgtatcacg ttctcccaag
     3181 gaatacaagc cccgcccccc cactccgcag gggagaggcc attcccatcc tgggattttt
     3241 ccctcttggg tcacatgcaa tctctggaaa cgaagacggg ccaccactca tccatgccgg
     3301 aagaaaaggt ttcctccagc gatggctgaa gctggtcgtg ccccaacacc tcttcttcca
     3361 ggcgtgaaag ctccaggatc ccttgccaaa cgacccctcg ttcctctccc gtgagcgaaa
     3421 gaagactcgg tcccctcatg ctttcaccgc ccttcgcccg cgctctgccc ttactctacg
     3481 ccatagttag gggcctcttt cgtgatggtg acatcgtggg gatgactttc tcgcatggcc
     3541 gctgcggtga tctttacgaa gcgccccttt tcttttagct cagacaccgt ggccgctccc
     3601 acgtagccca tgcccgcccg gagccccccc gctagctgat aaagcaccgc ccccagagat
     3661 cccttgcaag gcaccatgcc ctcgattccc tcgggcacga gcttctcttc gaaaaccccc
     3721 tcctggaagt atcggtcctt gctacaccct tccttcatgg cccccagaga tcccattccg
     3781 cggtagctct tgaaggaacg ccctcgatag atgacggtct ccccggggct ttcctcggta
     3841 cccgccaaga gggcccccac cataacggta tcggctcccg ccgccaaagc cttgaccaca
     3901 tcgccggagt agcgcacgcc tccatcggcg atgacacacc gtccccggga cttggtcgcc
     3961 cgggccgcct ccatgacggc ggtgatttga ggtacgccga tgcccgccac aatgcgggtc
     4021 gtacagatag accccgggcc cactcccact tttatgccgt cggcccccgc atccatgagg
     4081 gcctcagcag cctccgtcgt ggccacgttc cccgccacga tgggcaagtc ggaaaagagc
     4141 gatcgaagct ctttcacctt gcgaattacc cccgccgagt gcccatgggc cgtgtccacc
     4201 accagggcat ccactccggc cctcgctagc atctccaccc gctccaaggt atcgctcccc
     4261 gcccccacag cggcggccac ccggagcctg ccgtgggagt ccttgctggc attggggaaa
     4321 tccttcgcct tctggatgtc ttttatggtg atgagcccct tgagacggta gtgaccgtcc
     4381 actatgggaa gtttttccac cttgtggctc atgaggatgc tcttggcgtc ttccagagtg
     4441 gtgccttccg gagccgtcaa aaggcgatct ttggtcatca cttcctgaat gggctggtcg
     4501 tagtccgtca cgaagcgaag gtcccggtta gtgatgatgc ctaccaggcg tcgagaggcg
     4561 tccacaatgg ggacaccgga gatgtggtag tgctccatga ggcccaccgc atcccgcaac
     4621 aggttctccg gatgaaggaa gaaagggtct acgatgactc ccgattcgga ccttttcacc
     4681 ttgtccactt ccgccgcctg acgctccttc ggcatgtttc ggtggataac gccaataccc
     4741 ccctcgcgcg ccatggcaat ggccagacgg ctttctgtca ccgtgtccat ggcggcgcta
     4801 caaagcggca ggttcagctc aatgtccggc gtcaggcgcg tgcggaggtc cacctgggcg
     4861 ggcaccacct cgctgtaacc cggcaccaga aggacatcgt cgaaagtcac cccttcatac
     4921 gaagcaaact tgctttgttc actcatggat aggctcccct cccggaaata tttcggggga
     4981 gtgtagcacg aagggcttct ccctcgcaag acgtcgttcc atctccatgc ccttctcatc
     5041 ggcgcagggc acccgcaacg cctcccaagt catctttttg gcgtggcgcg ccccgatcat
     5101 tgctcccctg tcctgggaga tttcctgcgc cccagcagcg ccacggcgtg acacgccagg
     5161 gcttctcctc gaccaagggc tccgattttt tccgctgatt ttactttgac atggcagcaa
     5221 ggcccctcgc cgcagcgcag gacgtcgtcg agggctacgc ccatggcgtc acagaggggg
     5281 cccaggcgag gcaactgagc ctccaaggtg acgtcgaccc actcgggttc ccaaccctcg
     5341 tcccgcacgt aatccaccac gcgccccaag agatccatgc tgcgagcccc cttgtatcgc
     5401 tggtctgaag ctggaaagag gcgtcccagg tccgggagcc ccgccgctcc aagcagggcg
     5461 tccgccacgg cgtgagccac caagtcggca tcggagtgtc cgtcaagccc cagcggggag
     5521 ggaatggtca tccccgccaa aatcaggcgg cgctccggta cgagggggtg aatgtcaaaa
     5581 ccgtgcccca cgcgaaactt ctgtggtgga gcgaccataa gctccgccat gtgccaatcc
     5641 ccctcgtagg tgagcttgaa gttctctcgc tctccctcga cgggagtcag gttatgcccc
     5701 gccgcgatcc acgcctccgc ctcgtcacgc accgtgcaag gagatcgttc cagggcttcc
     5761 aaaaggcgct cccggaaaaa ggcctgggga gtctgggtgc caaagacccc ttcccgggga
     5821 agaggggtca cggtccctcc cgagaccttc tttagggcat ccgccacaga gaccagaggt
     5881 atggctcctc cggaggcctc ggcggcagcc tgaaggcggc gacagagctc cagggagcaa
     5941 aagggcctgg cggcgtcgtg caccatcacc caggcgccgc tggcggcccg aactccggct
     6001 aggacggaat ccccccgctc cgcccctccc tccacccgca cgacgggcac gacgcccccg
     6061 ggggggggcg tcgtctcgac gcccggatct gcgggcggca cgaccaaaac gcactcccga
     6121 aggtctccgg cggcgtgtag ggcctccgcc agggcaaggc tccaatgcca aaggggatac
     6181 cctccgaggg ggcgaaattg cttgggcacc ccccccatgc gggatcctct ccctgcagca
     6241 acgacgatca ggctccagtc cttcatgcgg cactctcccg acggattcgg ccaaagatca
     6301 tccttcccgc cgacgtctgg agcatggatg tcacggtcac ttccacccgc cgtcccacgg
     6361 cccgcccccc atcttccacg acgatcatgg tgccgtcgtc gaggtatccc accccctggc
     6421 tttgctcttt cccttcgcga aggacgtcca ccatgaggct ttcgccgggg agcaacatgg
     6481 gtttgagggc gttggccagg tcattgacgt tcagcacgtc gatgccctgg atttgagaaa
     6541 ttttattaag attgtagtcg gtggtgagga ttttcccgcc catgtcgcgg gcaagctgaa
     6601 tcagggccag gtccaccgtc tcggcgttca agtctttcag gctcagctca aggatctcca
     6661 gacggatcgt ggggattttt tgcaacccgt tgatgacgtc caacccccga cgccctctcc
     6721 cccggcgcac cgggtcggaa gagtcggcga tcccttgaag ttctaccaac acgaaacgag
     6781 gcagggtgag ggctccttcc acaaagcctg tccgtgccac atccaagatc cttccgtcga
     6841 tcaccacgct ggtgtctaat agttttgccg ggcacgccag cggagaaagg gtgccgccct
     6901 gaaggtccat cgttgcatat ttaccctgcc ttttcctcgg ttccccctgg cgatcccgcc
     6961 agatcccccc cacggaaccc atcatggcca ccatgtcgtc ccggcgtttg ataaagagcc
     7021 acatcatggc atatcccaag gctacgttca acaaaatggc caagtaacgc cccaggacgg
     7081 gaaggtctac gaagggcagt gccaataaat tcgccaataa taatcccacg atcaagccaa
     7141 tgaggcccac agaaatgttt atccaactga tattttgaag atgcccctcc agaagggacc
     7201 ccagacggag aatccctctg tggaaaagcg gggcaatcag gtaggatacg ccgccgaaga
     7261 ccaccacaaa ggaccccgtc cccgccaacg acatgagaat cgtctgaaag ggccaccacc
     7321 cggcggactg aatgaactcc gtcacttggt atcccgcaat tcctcccatg atggctaaaa
     7381 accatcgaaa gagttcccgg gccaaacggc ccagcgcacc gctattttct cccatgtacc
     7441 cccctccccg agtcaagcta tattctcctt cagtccggcc cggggctggc ctcgcccgac
     7501 ctcaccacca gccgggactc cagggccgca tggagagtaa ctcggtcggc aaaaccgata
     7561 ctccccccca ccggaaggcc gtacgccaac cgggtgatgc gaacggggac gtctgccagg
     7621 gcatccacca ccacgtggca agccaagtcc ccttcaatgc gggggttcgt ggccaccacc
     7681 acttcccgga cccggaggtc tcggatacgc ccccgtagcc gttccagggt ctccggagat
     7741 atctcctccc cttccagggg agataccctt ccccccagga caaagtactg accggagaaa
     7801 attcccgcct gctctaggct catcaggtct tccaccgatt ccaccacgca cagggtgtct
     7861 cgttctctca tggggtcgcc gcaaaccccg caacagtcgc catcggtgag gtttccgcac
     7921 tgagggcagg ggtgaaggga gcgccgaagg gtcaccaagg cctcggccaa ttggtccgcg
     7981 aaggacggat cctgttgaaa gaggaaaaac gccatgcgcc gggcgctctt ttcccccacc
     8041 ccgggaaggc gctggaggaa ggtcgtgagc cgggtcaggg cctccggaag gaccatagat
     8101 cagaaaagcc ccggcatgga cagacccatg ccccccgtca cctgttccat ctggcggttc
     8161 gctttttcct ttcccaggcg aagggcctcg ttcaccgccg ccagaaccag gtcttccaac
     8221 atgtcgatct cctgggggtc caccacttcc ggggcaatct tcaccgacag cacttccccc
     8281 tgggcgttgg ccaccgcccg gaccatcccg ccccccgccg ctccctccac cgtgaccttg
     8341 gccaattctt cctgcatctg aagcatctgg gcctgcatct tttgggcctg cttaaggatt
     8401 ttgtccattt tcatgctcat cgttcctttc ttttcgcgct cccgaagccc ggccagaggg
     8461 gcgtaaaacg cctcctcacg catggtgata ggggatatgg tgacggaggg tcaccgcgcg
     8521 atagatctgt tctaataaaa ccaccgcaca gagctcgtgg gggaaggtta aagacgatag
     8581 ggagaggcaa aatgcagccc gctcccgcac ggcggaagag acgccgtagg ccccccccac
     8641 gacaaacgcc aacctccccg gggatccctc atagatttca aaaagccact gagaaaagga
     8701 gacgctggaa aaggaccgcc ccccctcgtc cagcaagacc accacatccc ggggttggaa
     8761 gctccgaagg aggcggagcc cttcttcctc gacgcccttt gagcccgagg gacggtggtg
     8821 tgcctccgcc acatgctccc aggcgatcgg tgcccaccga gagaggcgcc gcacgtactc
     8881 ctgccccgcc tcctccaccc aacgcgcccg aggcatgccc acggtgatca tgaccatctt
     8941 cacggactaa accccgcctg cgctcccggc ggttcagtag ttttttgacg acgcagggcc
     9001 ttgcgaaggg tatagcgctc ctgtcgctcc ctttccacca acacgtcccg gatgcgccca
     9061 aagtcctccc gcaatccggg aagaactcgg tgttccaagt tgctcgtctt ccgcaccgtg
     9121 tcccgaaggg ccttctccag ggcccccatc ttcacccgga ggttgatgta ggtccaaaag
     9181 atatgatcta gctccgccaa ggctcccacg aggtcgtccg taaaaatcga cgccaaagcg
     9241 ggatcgtacg acaccggacg gctctcatcc ccctggcgac ggaactccac aaaggcgcac
     9301 cccatgagca cgtggcgaaa gcttgccaag ggaacgggac ccatccccgg acgcaggaga
     9361 tccagggaaa tctgtccttc gtagaggcgc aagagggaat acgtcgtggc cacccggcgt
     9421 ccgaccctat cgaactgctc ctgaagggat ctaaaacgtc gccgatcctc ttccagggcc
     9481 cgaagcaagg catctctttt cctttcgaga agggccttcc ctcggcgcac cgcctccatt
     9541 tgctgtcgca gcaacaacaa ctgctctcgg gtggcgggaa gggccacggc tagcgccccc
     9601 ctttctcccc caagatctct cgcaccaagt cgcggggcag acgatagagc tccgaggcgg
     9661 ggagatctcg gaggcactgc cacgcggcct ccagagacct ctctagcgtc cgaggtccct
     9721 ccggctggcc tataaaacgc tcttcgaagg actttccgaa gaccaaatag gatcgctccg
     9781 tggaagaaag gccctcgtct ccggtgatga ggcgcaatct ttcgatatcc tgcgccttag
     9841 cgtaggccgc atagagctgg tccgccatgg cccggtgttc ggggacggta cgccccttgc
     9901 cgatgccctt gttcatcagc cgactcaggc taggcaacac ctgcactggg ggataaatgc
     9961 cccgatccgc caaccgtcga ctcaggacca cctgaccttc ggtgatgtat cccgaaaggt
    10021 ccaccacggg gtgggtcata tcgtcgtcgg gcatggtcag gatggggatc tgggtcacgc
    10081 tacccgccag gccctccacg caccccgacc gctcgtagag ggacgccagg tcggagtaca
    10141 tgtaaccggg atagccccgc cgccccggga tctcttctcg agcggcgctc acctctcgaa
    10201 gggcgtcaca gtaactgagc atgtccgtga ggatcaccag gacgtcgtac cccagggtga
    10261 aggcgaagta ctccgccgtg gtcagggcga tgcgaggggt caggactcgc tccaccgccg
    10321 aatctcctgc caaattcata aaaaacacac ccttggccag ggctccggaa tcttgaaagg
    10381 cgctcaaaaa gaaatcggcc tctctcctcg tgatgcccat ggcggcgaag acgaccaaaa
    10441 agggccttcc cgttccaggc acccgggccc cggcggcgat ctgggccgcc aggcgattgg
    10501 ccgggagtcc cgccccggtg aatacgggca gtttctgtcc ccggaccaag gtgttcatca
    10561 cgtcaatggt agagatcccc gtctccacgt aggcgttggg agccttcctt cgggcgggat
    10621 ttagcgccag cccttgaacc gacatgcgac cttcggcata ggggacctct cctccctcca
    10681 ggaggtgtcc ccggccggta aggactttcc ccatcaaccc tggccccacg gggacggtca
    10741 aaacggattt ttcgagccag atcgtcgtgg agctttcgtc caatcccgtg gtgccttcga
    10801 acacctggat gaccgccgtg tcccggtcca cttgcaatat ttggcccgat cgggggcctt
    10861 cgggggtttc gatggtcacg agctctccat aacccgcatc ccgcacgccc tggacaaaga
    10921 tcaacgggcc ggaaatgccg cacacactcc gatacccttc gcggtagagc gcctcctgcc
    10981 cggaagtcat gcgggcccca cctcctcaga tgccagctgg gccttgaggg attggataaa
    11041 gagctcgcct tcggaggcca acccctcctc ctccaaggat cggagtcgaa aaagctcgtc
    11101 gcgcaggaac gtccggggca cctggtccgt tcccaagccc tgcctgagac gttggaccac
    11161 ctcctgatcc aggttccgga aaatatcgag cagcgccttg gccttgcgca aagaggtgaa
    11221 ggcatccacc gcatcaaagg cgttttgctg gaggaagacg atccgtagga gctctgccag
    11281 ctccaggagc cacttgtcct cctccgacag gccatcccgc cccacgagct gtaccaactc
    11341 caaaagctcc ttttctcggt ccagcgtggt gcggagaaag tgcctctttc cctcccaatc
    11401 ctcccccagg gccttcagaa agtggtgggc caaagaatct tcatagagcg tgtaactcga
    11461 cctccaatta atggccggga agtggcgctg ctgcgccagg gtcttatcga ggccccagaa
    11521 ggccccggaa aggcggagac aggactgggt gacaggctcg gaaaagtccc ctccgggggg
    11581 agagacggcg ctgatgacgg tgaccgaccc ctccctctcc ggcgtgccct gggccacgac
    11641 cctccccgat cgctcaaaat actgcgacag gcgggagggg aggtaggccg gatacccctc
    11701 ttctccgggc atctcttcga gccttccccc gatctcccgc agggcctcgg cccaccggga
    11761 gagggagtcc gccatgatgg ccacgtggcg ccccatgtct cggaagtact cggcgatgct
    11821 catccccagg tagatggaag cctctcgggc cgccacaggc atgttggaag tgttagccac
    11881 caacacgacc cgctccatga ggggccttcc cgtgcggggg tccatcagtt cgggaaactc
    11941 ctccaggacc tcggtcattt cgttgcctcg ctcgccacag ccgatataca cgatgacatc
    12001 ggcgttgcac caccgagcca gcgactgctg cgtcacggtt ttgcccgtac caaacccccc
    12061 gggaatgacc ccggcccccc ccaaagccag gggaaagagg gtgtcgagga cccgctgtcc
    12121 cgtctccagg gggttctcca agggcaaata gcggcggacg gggcggggtt ggcgaacggg
    12181 ccaagggtga aagagggcat agttctcccc ttctcgggtg gtgcagacgg tggcatcggg
    12241 ggaaaaggat cccgcagggg ccacgctttc caccgtccct ccccgggccc ccgggggtac
    12301 catgatggcg tgaaggaaat ccgcgccctc caccacatgc cccaggatgt ctccgggatc
    12361 cacgcgatcc cccggagtca ctttcggagt aaaagcccaa gaggcctccc gatccaacgt
    12421 atccctccgg gctcctcggg ggagaaagat cccttccctt cccaggggca ccagagggcg
    12481 ccctatgccg tcaagcacgt tccccagtag ccccggtccc agctccaccg agaggagctc
    12541 gccggaaaac cacaccggct cccccgtgcg aagccccatg gtttcttcgt aaacctggat
    12601 atctaccacg tcgccccgga ggcgcaccac ttccccgatg agctcctctt gccccacccg
    12661 ggccacctca aacatacgga cgggcccctg ggggcgagct cgcaccaccg ggccggaaac
    12721 gctcaaaatg gaaccgctat gccccataaa atcacctcag agaccctggg atatcatgtc
    12781 ggggacggga atccacgccg gacgaacctg ggcgaggatc ttcctacgcc atggaggagc
    12841 gagttgggca aaccatcctt cttcgacgac aacaagacaa acgtcccccg ccgtaagctc
    12901 gggccacact tccgccaggt cgccggggac atcgcaacag ataggacgaa ggccgttcaa
    12961 ggcccagaga tccacgaaaa ttttcctacc cagggccaca ccgaattctt tcatgcgtcg
    13021 ggctccataa aaagaagggt ctcttggatg cggcgcgagg gaagccctag cgccatgccc
    13081 accgccacac ggttgaggtt ctgttcttcc aaaagctgaa gccctatgaa acctatgccg
    13141 acgtcgatgc ccagaaggtc tttccgaaaa agacttgcct gccaccggag aaaggactcc
    13201 gcgaaacgct gctgggtcct gtgtggggag aagggggcac ctcgccgggc cacgtctcgc
    13261 cagcaagtgt gggacaccag ggcttcgagg gtagaagatc gacgcaggcg atccagggaa
    13321 aggcaagccc caccggggac gtaggcctgg acagattccg acgccgacaa gtatcccgcc
    13381 ctctccctga gccaaatccc actgttccag acgtcggcga ggcgccccag gtactcccgc
    13441 acccggtcgg cccccgagct cttccaggga tccaaggccc tgcgaagaaa ctccaggtac
    13501 cccagcaaca gatgccgctc tgcccggggc aggcttccgt ggcgcatcag gtcctgagaa
    13561 gcctcccaaa gcacccctcc aaaggggtga ggtatccctc gacaacggct ggcgaattcc
    13621 tgtagatcat ggctctccca gagctctcca aagagcgccg gggaaagcac tccgaaggag
    13681 gcccaaaagg gcatctcctt gggggatcga tggggacttg tcatggatcg caacatcagc
    13741 cgggcgtttt gaaaatcggc ccgagccagc aagatatcca ccagaaccgc cgtctctccc
    13801 tcggcccgac gacgcaggac atcgaaggac tgagcggtac ccagcgacag cgtcttttcc
    13861 acgcgacgaa aggcggaacg ctcggaagtc accagccgag agcgcaacac ttccgcgtag
    13921 cggccgtcca gcaaggccac ctccaagtcc tccaggaccc cgtccgccag gcgccgaagc
    13981 tcttcggagg aaaaaaagcg agccccccgg gaccggaggc gagcattaat ataggcgtag
    14041 ttctcgggcg aatcgctcga cgtcgtcttc ctggccaccc agggccattc ccagttgagc
    14101 ggcgaagaaa gcttcgctcc ggctccatcg ggtgcggaag gtgttgtcca ccatgtggcg
    14161 cccgtctgcg gcatcgcgac tcacgcatcc tccccaggga tcttttacaa tcccctccac
    14221 tcgctccacc cccgggagct ccctcaccgc gtcttcgtct cccggaggca ccgccagcac
    14281 cgctttgccc cgcatacacc ccagagcttc ttgggccaaa aggatcagcc atcggcgcct
    14341 ctcctcggca tcggcgttct ggaagcggaa ttccacctgg gccatcaccc gggccacgag
    14401 ctctcgcacc acttccatcc tgaggtggcg gtatctcccc agggccctct ggcgcaggat
    14461 ctgttcctga cgctcgtgga cctctcggcg atcctggcgc caccgggtga tgtccttctc
    14521 ggcctggctc cgccgactcg caatcatatc tgaaattcgt tcttccgcct ctcgacgaaa
    14581 cgcggccttg cgctcctcgt gttcccggcg catctgttcc ttaaaggcga cgagagagtc
    14641 tttcaaggaa ccgcctcccc ggggacctta acctaacttc accagaacca tgacggccac
    14701 cacgaagccc aggatcacca tggtttcggg gatggcttcc aggatgatca tggtccccgc
    14761 cgtttccggc ttttccgcta cggtccccgc cgctgcgctc ccgatctttg cctgggcgta
    14821 gccggtggcc agggccggga ggccgatcgc cagagttgcc gcaaacgcta tgagtgcctt
    14881 ctccataaaa cttctctcct cctttggaat gggtcgtact cctttccccc tccggtgaaa
    14941 aacttgctga aaaactccac gtagtgcaac cgggcggcgt ggagcccgga ttcaaagacc
    15001 gccaggacga tgttcagggc gtggatcgat agcgccatga aaagccccaa gacggagacc
    15061 cccaagaggt ccacgaaggt ggacgccacg gaggcgagga tggcggaaga aaccccgatg
    15121 gccgcgatcc gcacgtagct caggacgttg cccacggcct ccaccccctc caccatgccc
    15181 cccatgcccc ctcccaccaa tatcagggcg acgccccccc ccagaagtat cagggcacca
    15241 agaaaggccc tttctggaat cagggcggcg tatatggccc ctacaaaggc caaccccagg
    15301 ataaggaaga tcgtgcccaa acgctccatc caccggtggc gatggcgatg cctcaaggcg
    15361 tcaaaacatc cgatgcccag gccgatgagg atatggcccc cccccaagac cacggaaaag
    15421 gccaaaaggg gcatcaccgc atgagctcga tccacccata ggggagatag tcccaggaga
    15481 cgccacccca cgtcgccgaa atactcccca aacaccacgc cccagatgaa gctccacccc
    15541 gccacgtgaa gagctacagc ccctgcggaa cggatcaccg cgccccccgc agtgcggcgc
    15601 ctcaagaaaa atcccaccgc gccgaggagg agcgcatacc ccgcgtctcc cacaatacac
    15661 cccgcaaaca gcgggaaaaa gatccccacc aacaccgtgg ggtccactcc catgtaccga
    15721 ggggacgaaa agaaatttac aaatatttca aaaggggcga agagtgaggc gttgcggagg
    15781 gccgtgggga cctggtgcca ttcgtcctcc atggcctggc gccaaaaaag aaagagtttt
    15841 tttggaaaac ggcgctgcag ggccgccacg gtgtcctctt gccgatcttc cggcagccac
    15901 ccctcgagat gaaagaggct tccctgggcg gccaaggcgt tttccagcgt tttcacctcg
    15961 gtcttttcgc tgaggagggt aaaaagggcc cccagacgca tcccccactc gtcgcgggcc
    16021 tggtctatct cctgctggat ctgggacagg cggcggggaa cccactggaa gccgtcctcc
    16081 atggcccgca ggacctccgc catactatcc cggtcgaagg caggcgtggg gcgccaggag
    16141 gctcccccgt cgtggagcac caattcttcc aacacagaac cttccgaatc cgcaggcaaa
    16201 gacacggcga agagctcgtc ccgttctgtg ctgggctggg tatgacgatg aaatcgaagg
    16261 ggatctcggt cgttccacca tccctttcga gtccaggcgt ctcttatcat cgcccccata
    16321 cgtcccgccg aagcctgggg tacccagaaa aggagcgcca tcctgttttt actcagggcc
    16381 tcacggaaga agttctcgaa gggggccagt tccctctgca tgcgctttag gctccggagg
    16441 gtctcctcgt aagattctcg ctccgccatg agcccggcca atcggttccg aaagacctcc
    16501 acgctctttt ccaaccccac caaggtggac tcttggccca catcgaggcc cagggatgtt
    16561 ctatctagaa tttcatcgga aaggagctcc catcgatccc accgcagggc ttccaggatt
    16621 ccctggagcg tggcgcacag gatcttgagg cggcgtaggg tatcccccag gggagctcct
    16681 tcggtcccca tctccacgtg caggactccc aggcgctgca gctcctccaa gacgtccacg
    16741 cggcttacct ccgtgcccca cacggaaagg cggatcatcc gacggatcac gacaccattc
    16801 cctccggcgg caacggcacg atgcggcacc acatcgcctc cgccaaccgc agaaccatgg
    16861 cctcctgcag aaactgggcc ttccactcct ccgcccgaag agtcacgagc tcatccaggc
    16921 gtcgtcgctc ctcttccaag gcttcctttt cccggtcctc ttcctggcgc atattttcca
    16981 ccgtggcccg ggccatttcc tccacccgaa gggaggcacg atcccattct tgctgctcat
    17041 gtttacgcaa ctcttcaagc tcttcttcca gggactggcg gagggtctcc agttcccgcc
    17101 gcaagatctc ctggatttcc cgagaattat cggctcccat ggcttattcc tcgcgatcca
    17161 tcatgaaccc acagaaagta gcccggtcca ccacccccac caggcgcccc tcttcaacca
    17221 ccgggaggcg tttcaagcgc tttcggagca tcaaatccgc caccgccatg aggctggccg
    17281 aagtgtccac gctgacggga tcgggacaca tgaactcctt gacccgacgg cgccccaatg
    17341 cctggaggcg atcaaaaagg cttccttcgg aattatccag aaaggaatgc tgcatgagga
    17401 cttccacgta cgtgggcaaa gcggcgcgca ggatgtcgcc ttccgaaagg taccccgtga
    17461 ggatccagtt tgatcgtacg acgggaatgc cggagatacg ctgcgagtgg aaaatcctta
    17521 cggcttctag aatagagtcc tcctcttcga cggcggaaag atccttagtc atgacctgag
    17581 ataccatagt tttcacgtat ctctctccct ccgaaaacgg tctaggccga ggcgagtctt
    17641 gaccagagct cctaagaaaa gtggtatagt catcatccgt cgccaacgcc agggttcctg
    17701 agcgaggcgg tagagccact ccaggccata gttccggaaa cattccgggg ctctcctcag
    17761 agtcccggca aagacgtcga agctcccccc cactcccatg gccaccacgc cgccgagatg
    17821 gccgcgccag agctccatcc agagctcctg tcggggcacc cccagagcca cgaagaggag
    17881 ctgcgacccg gagttgcgga tggtacgaca caactcttcg ctctcgtcgt cggcaaaaaa
    17941 tccgtgctcc gcccctctca ctcgaaggcc agggtaacgc ttcacgaggt tttgcgccgc
    18001 catctcggcg actcccggtg cccctccaag aaagaacacc ccccaccccg cccccgccgc
    18061 catccgacag agctgatcca tgaaatctat ccccgcgatt cgctcctgca cgggtctccc
    18121 caaaaaacga aggccccaca ccaatcccat gccgtcgggg aggatcaggt cggccccctg
    18181 aaaggcccga gtcagggctt catttcttcg acaggcgtag agccccaggg cgttgggcgt
    18241 caccaccgtg ccccccttcc ccgccttgac cagggaccaa acttttccca gggcgtactg
    18301 gagggagacg ttgtcgatgg ggacgtccca aagtttaggg cggcggcttt gccccttgcg
    18361 gccgtgacga cagaacacca tccccaggta ggccaaggcg gcggatatgg tgaaaaaggc
    18421 cgccaaaaag ccctgggggt cggggcgcaa ctgcaacacc ccggccccca gcccgaaggc
    18481 ggcggagacc aaggtcatgg cccgcacaga gctggggtga tccacccctc gacggaccaa
    18541 gcgctggtaa aatcccccgg aggttttcgt cgcgtcggcg gagagggctc ggctgaccac
    18601 gttcaccgag gtctcccaga gggggatggc aaaaagggcc aaaggcagga gcataagcgc
    18661 ggagaaggtc acccctttgc tgatgcctag catggaggta cctgcgatga gcgtacccca
    18721 catggaggcc aagggtagcc ccaattgacg gtacatatgg ccgtggcggc tccagaacgc
    18781 cgccaggaga gccaaggccc ccgtgctcaa ggtcagcgcg tcagggagct cctgggaaga
    18841 gatcgccgtt agccctgtgg tcaagatcca aaccaacgcc agcaggtgtc ccgccatgcc
    18901 gggtatgcgg tctagatctt gcaacaacag ggggaaaagc cccacccata gcgtggtggc
    18961 cgtggcggaa gcgaggatag agaggtagta gaactccccg tcgggaaggg atatgaagct
    19021 gatccggggc cccaaaaggg aaaaaaggcc tccaatgagg agataccccc accgaagatc
    19081 cacctgggct cggcgctgcg ccaagcccag cagcccggcg aaaagggaga gggccaccac
    19141 catcttcgcc ggggcgctgt cgctccacaa agccaccaag gcgagggccc cacagagcat
    19201 gaggtcccgg acatagtcat actgatctcg atccaagtga tgacgcaaaa atcgctgggc
    19261 cccgtagcac cccagcacca ggaggagcgc cgcgcttctt gttagcagct cggcaaaggt
    19321 ttgaatagcc atatcccttc ctccttcctt gccgaagatc cttcacacca caggcccgga
    19381 gcctcaactc ctccctccgg aaacggcgac atctcttcgg atcacggggg aagtatacca
    19441 caccacatct cccatgacga ggacgctcgc ccccctgaga ggcggaccga cctaaccccg
    19501 gcgagggggg cccggccctc cggagacggc gctctcgccg ccccggagag ggggaccacc
    19561 acccgggatc ggggcctatt accgccccag ggtctcctga ttccccatcc agggcctgag
    19621 ggccccgggc acctctacgg agccgtcggg ttgctggtaa ttttccatga tagcaatgag
    19681 gcatcgtccc accgcaatgc cggatccgtt gagggtatgg atgaactgag cctttcctcc
    19741 cttcgtgggg cggtagcgag tccccatgcg gcgggcctga aagtcctcgc agttgctaca
    19801 ggagctgatc tcccggtatt tgccctgaga gggaagccag acctccaggt cgtaggtctt
    19861 cgccgaggcg aagcccatgt ccccggtgca aaggcacacc acccgatagg gaagctccag
    19921 taactgcaaa accctttcgg cgttcgccgt gagcttttcc agctcgtcgt agctcgtggc
    19981 gggggtacaa agcttcacca gctccacctt gctgaactgg tgttgacgca agattcctcg
    20041 cacatcccgg ccgtaggacc ccgcctcttt cctgaaacat ggcgtatagg ccgtatagag
    20101 cttgggaagc tcctcctccg gcaggatttc tcccccgtgg aggttggtca ggggcacctc
    20161 ggcggtgggg atgagccaaa ggtcgtcctc cgcacagcgg tagagatctt cggcaaactt
    20221 gggcagttgt cccgtgcccc gcatggtggc gctattgacc atgaagggag gggcaaactc
    20281 cgtgtaaccg tgctcccggg tgtggaggtc gagcatgaaa tttatgagcg cccgttccat
    20341 gcgggccccg gatcccttta aaacggtaaa ccggctctcc gccagccgca ctcctctttc
    20401 gaaatccagg atatccagcg cctctcccag ctcccaatgg ggcttgggct caaagtcgaa
    20461 gctccgaggc tctccccagg atcgcaccac cacgttgctc tcttcgtcgg caccccgggg
    20521 caccgagacg tggggacggt tcgggatctg ggacaagagc tcctccatct ggcggtccag
    20581 ggaggcccct tcttcgtcga tggcccccac ccgctccccc aaaaggcgca tctcctccat
    20641 gagagcatcg gcattttcgc cctttttttt agcctccccc acccgacgcg aaccttcgtt
    20701 tcgtcgagcc ttgagctctt ctccctctcc caggagatcc cgccggcgcc gatccacctc
    20761 cagtagcgtg tcgaggggaa aggcgctcct ccgttgggca agcatctccc gtacttcgtc
    20821 cgggcgctcc cgaacccact taatgtctaa catggctatc cctcccgagg agttctgatc
    20881 tcccgtagga gattggccat ctccagcgcc gccagcgccg cttccacgcc tttgttgccc
    20941 gccttgctcc ccgcccgggc caatgcctga tccaaagaat cgcaggtcag cacaccaaag
    21001 gccgccggaa cccgctgatc cagagcgatg tgagccaatc ccttggatac ctcggaggcc
    21061 acgtagtcaa agtggggcgt gtctccccga atcaccgccc ccagggccac caaggcatcg
    21121 tggcgccctt gaagggctat ttccttcacc gccaggggaa tctcccaggc cccgggcacc
    21181 caatacacgt ccaaatcctg atgtcgaacc ccgtgccgca acagggcgtc tttggccccc
    21241 tccaagaggc gtgacgtgat cagctcgttg aacctggaaa ccaccagggc aaagcgcaag
    21301 tcgtttccca gaaattttcc ttcaaactgt cgtatgattt gtccctccta attcatgttt
    21361 ttttatgctg cagtgagcct accccaggac atcttgcagg tggagtaggt gtcccatttt
    21421 cttccgtttt gcctctaagt atcgctcgtt gtgagggttc gggtcgactt ccaggggaat
    21481 gcgatccgtg atcctcaagc catatccctg caaccccgcc accttccggg ggttattcgt
    21541 caggaggcgc aaggaacgga gccccaggtc ggccaaaatt tgggccccca ggccgtaatc
    21601 gcggaggtcg ggagcaaagc ccagggcttg gttcgcctcc accgtatcga ggcctcgctc
    21661 ctggagttca taggccttga gtttagagag caaacctatg ccccgccctt cctggcgcat
    21721 gtagagcacg acgccgcatc cctcccggtc tatgcgctcc agggccctat ggagctgggg
    21781 gccacaatca cagcgcaacg acccgaaaac gtcccccgtg aggcactccg agtggactcg
    21841 caccagcacg tcctctttcc cccgaacctc cccctttacc agggccaagt ggagtcgctc
    21901 gggggtttcc tccaggcacg accgataggc catcgcgacg aactccccgt gctccgtggg
    21961 gagctgaacc gtggccaccc gctccaccag acgctcgcga gccgtgcggt gttggatcaa
    22021 ctgggcgatg gagaagaggc ggagggcgtg ggtggcggca aaactccgaa ggtcgtccaa
    22081 gcgagccatg gatccgtctt cgttcatgat ctcgcagatg acccctgcgg gcgctagccc
    22141 cgccatgcgg gtcagatcca ccgccgcctc cgtgtgcccc gcccggcgca acacccctcc
    22201 ggggcgagcc ctcagaggga aaatgtgtcc cggcatccga aaactctccg tcttggcgtg
    22261 gggcgaggcc agcagagcca ccgtgtgcgc ccgttccgcc gccgagatcc ccgtggtggt
    22321 accatcccga gcgtccaccg acaccgtgaa cgccgtgccg tgggggtcgg tattccggga
    22381 cgccatgggg gcgagcccca gggcgtccgc tcgctccggc gccaggggag cacagatgag
    22441 cccccgtcca aaggtggcca tgaaattcac catctccgga gtggccttct ccgccgccgc
    22501 caaaaggtct ccctcgtttt ccctgtcctc gtcgtccacc acaatgatca tgcgccccgc
    22561 tcgcagctct tccaaggcct cttccaccga gcaaaacaca gacccctcat cagcgcccat
    22621 tatttttcac ctcgtttttc ccccgtcacc gcttcccggg gaaatcgtga ccctttgaag
    22681 ggagccccca gggcatcccg atccatcata tatcgccctc ggccccttct caagggcctt
    22741 cgaaccctat cgctccacgg cagcccgacg ttggcaagcc accgctccgg tctccggcgg
    22801 gacgccctct agctcccggg ctccgccaga agccccttcg ggaccggtat ggtcttaggg
    22861 cgtccatccg tattgggcga ggcgctccca ggtgaggggg gcgggcgttg aaggtccctc
    22921 gccccctagg cgcctcgcca ggaagcgctc cacgtacttt cccaacaaat cggtttcgac
    22981 atggagctcc gcccctggag cgatcgttcc cagcgtagtg tcccccaagg tggtgggaat
    23041 gaggcccacg cgaaaaaatc ccccccacac ttcggccacc gtaaggctca cgccgtcgag
    23101 ggccaccgac cccttgggca cgaggccatc gatgatggca gcttcagcgg tgaggaagag
    23161 ctctcgggtt cgtcccctcc cccgcacctc tttaaccacc gccaggccat ccacatgccc
    23221 cgtaaccaca tgtccatcca atcgccctcc cagggaaagg ggcctttcca ggttgacccg
    23281 atctccgggg cgcatccgac caaagcgcgt acaccgccgg gtctcgggca tcacctccgc
    23341 cacgaaggac ctggagcccc cttccaccac cgtgaggcac gcccccgaca ccgccaccga
    23401 ctccccccgg cgcacctcgg gcgccagggt cggagcctct acgtccagca gcagcacgtc
    23461 gtcccgggtc cgaagccccc ccaccgtccc cacgctttca atcaggccag tgaacacgat
    23521 atcagccctt ccagcaagag gtcctccccc aggggagtcc agagggggtc ccggagcttc
    23581 accgcttcgt ccatggcccc gatggtcagg tcgccggaaa aggggagccc tcgccccaag
    23641 agcttggggg caagaaagag cgccacgccg tccaccagcc cttcctggaa aaaggaaccc
    23701 agcacgctac ttcccccctc caccaagagg gacaggacgc cgcagcgggc caaagcggcc
    23761 aatacctccc ctaggtcgag ggctcccggg accttttctc gcaccgccac cacctccgcc
    23821 cctcggcgtt gcagggcctc ccgccgctcc ggaggggcct ccgggccgca gaagatcagg
    23881 ggcctatttc cgttgagcac ggcggctcct tccggcaggc acaacgcccc gtccagcacc
    23941 acggggcgcg gcgaacttcc ctgggcctcc ctcaccgtga gggccggatc gtccgatcgc
    24001 accgtcccga tgcccaccaa gaccgcgtcg tgctcccttc ggaggacgtg agacacccgg
    24061 cgggaccgct cccccgtgat ccacttactc tgtccatcgg ccaacgccaa ggagccatcc
    24121 cacgagcagg ctcccttggc ggtaacccag ggacgccccc gggttaaaac gttaaaaaag
    24181 cctcggttta tttggcggca gaggggttcg agcacccctt cggtgcagga cataccccct
    24241 tctcgaaggc gacgcatccc tcccccatcc accttggggt tcggatctcg cactcccacc
    24301 accacccgac caacccccgc ttccaggagg gcatcagcgc agggaggggt tttcccgtgg
    24361 tgggagcaag gctccagggt cacgtaggcc gtgctccccc gggccctttc tccagccctc
    24421 cgcagggctt ccacctcggc atgggggcca ccgtaatagc cgtggaaacc ccaacccacc
    24481 acggaatttt ccctcaccag cacacacccc acccggggat tggggctcac cttccccgtg
    24541 cctcgcaggg ccaaagagag ggccatccgc atgtaccttt cgtcccgcca tcgttccgat
    24601 cgcgtcatga aaacacagcc tttcttgatg aaagggctcc ccccagagga tcccggggat
    24661 cgccggatcc cccgaggccc cttgccgcca gccccatggg gctaaaaggg ggcagaaacc
    24721 gccccgggag gactccgtca cgccgggcct tctttaccat cctctccccc cccggagggg
    24781 aggccttcgc cggagggggg agagccctta tcttctttcc cttcgttccg ggggaggtcc
    24841 atggcccgcc acaacccctc caagatcctt ttcgccagtc ccgggccaat gcccggaata
    24901 cgggccagat cggcggaatc cgtggcggcg atccgggccg gagatcccag ggcccccaaa
    24961 agagccgccg ccctcttctt ccccactccc gggatatctt ccagcgccga gcggcggagc
    25021 ctgacgtccc gaccccgacg gtgcgaggat acggcaaagc ggtgtgcctc atcccgaacc
    25081 ctttgcaaaa gatgcaaggc ccggtcgctg cgctcaaggc gcatcgggac ggcctcgtcg
    25141 gggcggtaga tctcctcatt cctctcggcg agggccacca tggggatatc tcccaacccc
    25201 gcccctcgaa gggcatccag ggcaaaggcc agctgctggg ggcctccgtc gatgaggatc
    25261 agctgggggg ccggttcctg tccttgcacc acccgacggt aacgccgggc caccacctct
    25321 tccatcgccc ggaagtcgtc caccgccccg tccaaagatt tcaccacgaa acgacggtac
    25381 agcgagggat tcggagcccc ctgctcgaaa accaccgcca ccccataggt ctccttgccg
    25441 ctgaaatggg agatgtcaaa gccgtcgatg cgccaaggca aggtctttag cccgaggagc
    25501 tcctgcagct gacgcaacac ctgccatgtc tccccgtcta aggtgttatg ccccgccaag
    25561 ctggtgcgct ggcgcgtgta gtgccacaac ccccggatca tgtccctcag gtgagccgcc
    25621 tcttcaaagg ccatctcctg ggccgcccga tccatgcgcc tacgcagacg ttccaccagg
    25681 tcggcggtct tgccggacaa aaggagcacc agatctgcga cccgctctgc gtaggctcct
    25741 tcagagcaga ggcccgcgca gggacccagg caacgcccca gggcgtggcg cacacaggga
    25801 cgggcctcgg gggctccggg gtcgatctcc cgggcgcatt gtctgagagg aaaatacctt
    25861 tcgctcaggc ggaggagttg gcgcacctcg gccacccggg taaaggggcc aaagtagatg
    25921 cctccgtcct cttccctgtg ccgagtgatg agaatccggg gaaagcgctc cttggtgatt
    25981 ttgacgaagg gatagcgctc tcccatcttg agctccacgt tgaaaaacgg ctgatacttt
    26041 ttgatgagac gagcttcgac gatcagggct tccacctcgg attccgtgcg gatcacggag
    26101 atatccgcaa tggaggccac caaccgtcga agccgagggg aggcgtagtc ctgcctccgg
    26161 aaataggagc ccacgcgttt ttttagcgat ttggccttcc cgacataaag gacctctccc
    26221 tggtcgtcgt gcatgaggta gacccccggg cgatccggca accctcggat tttttctcgc
    26281 agcgcctccg gcgagagctt gttccccatc tgtgcacccc gatccctaga ggcgttccac
    26341 cccttccaac tcgccgctcg acacctttcc ccccaccacc gtggcccggg ggatcacgtg
    26401 gcgcagcctt tcctgaggta gggcaaagag gtcctcgccg cagaccacca cgtccgccag
    26461 aaaaccgggg gccaggcgcc ccctacgccc cccttctccc accgccaacg gaggattgac
    26521 agtgaagagg gtcagcgcct catcaaggga gagcctttgt tccggggtcc aacctccggc
    26581 ggggcatccc tcgtcgtccg tgcggcacac ggccccccat atccctcgcc acggggaagg
    26641 cacctccaca gggcagtcgc tggacccggt caccacgaac cccgcatcca cgaaggtctt
    26701 ccagggatag gcccaggcga ggcgatcttc ccccaggcgc gacgtggcca tggtgatctc
    26761 cccggggacg aaggcgggct ggacgtcgac gaccaccccc agctcccgca ggggttccac
    26821 ctgatccgga cgacagatca tcacgtggtt cacccgatgg ggccgggaga agcggccggg
    26881 ccccagggat tttatccggc gcaacccctc tatggcctga tccagcgccg catcgccaat
    26941 ggcgtggatt tccatctgaa agccccggcg atgagcctct tccatggtgg ccatcagggc
    27001 gtgatcctcg tggttcagga tccccgacgt ggtgggagcg tcggcgtaag gccgggaaag
    27061 ggccgccgta cgaccgccga gggagccatc cagaaagagc ttgaaccctc cgaagcgcac
    27121 ccagtcgtcc cccaggccac tgagaaagcc cgctggaggc atgtcatccg tatagaacac
    27181 cacccgtagg ggtaacgccc cctccaagcg catctcctga taggccgcca agtcatcccc
    27241 cagcccgtag gagcccgcgc tacaggtgtg gacgctggtt attccgtagg aggcaaactc
    27301 gaggcaggcc tcttccaggg cccggcggca ttccttggga tcgcgatctc gctccaccgc
    27361 ctggatcagc ggatctcctt ccccttcgta caagatgccc gaaggcacgg cgtccggggg
    27421 aaaccccgcc gccgcgagcc cccggctgtt cgccacctgg acgtgacagc agatgcggga
    27481 aaggagcacg ggattcggca cccccaaggc gtccaagtcc tgcctcgtgg gcaggcgctt
    27541 ctcctcccat tgggtatcgt taaaccgaac cccacagatc cacgagtcgg ggggcactgc
    27601 ggcgctcctc tctcggagga gtctttttac gtcttccagc gaggaacacc ggtccagggg
    27661 caccgcgtga cgctgcttgg cgtaaaacgc caggtgggca tgggcgtcgg tcaggccggg
    27721 gatcaccgac tcccccccta gagagatgat ccgggcccgg gacgccaggg ggtgagaccg
    27781 cacctcttcc tcggatccca ccgcctccag ggtgtctcgg taccacaaaa gggcctctgc
    27841 ccggggaacg cgaggatctc ccgtccatat tcttcccccc cgaaggagca ccgcagaagc
    27901 ctccgactca cgcatggcca tcgcccattc ctcccgtcgc ttcccgtccc ggacgcccgg
    27961 gggggtcacc ccttatccgg gagaagcctc tatggccgga acccggccaa ggcctatcgg
    28021 cgagggacgg ccccggcgcc gatcacgccc tccggaggta ttctagcacg ccccccggga
    28081 aagggggatt ttgggaagga tcctggggct ggcgagcctg gaaaatccct aatccactcc
    28141 tcttccacgg cccttcaccg cgaggccggg acatgacccg tcccctcgcg ccgccttccc
    28201 tggcgccggt cgagggggac ggagcttccc gccccgtcgc cgggcccgag cctccgcccg
    28261 tggggccacc cctccggttg atcggagggg cctttgcaaa ttggcctttg ggggatagaa
    28321 ttgctttcag aaattcttcc ggaggtgtga aaaatggccc tgaccttgtt taacgacttg
    28381 acaaaacgaa aagaaccctt tgtgcccctc caaccgggga aagtgacctt ttacgtctgt
    28441 ggccccacag tctacgactt cttccacatc ggcaatgccc ggcccttcat cgtcttcgac
    28501 gccctcaggc gacacatgga gcagagcggg tatgaggtca cctacgtcca aaacttcacg
    28561 gacatcgacg acaagatgat ccaccgggcc cgggagcttg ggatctctgt ctccgagctg
    28621 gcatcccggt tcatccaggc ctacgaagaa gacgccaagg ccctgggcat ccgccccgcc
    28681 accgtccacc ccaaggcgac ggagcacatc ccggaaatcg tgaccatgat ctccgaaata
    28741 atccgccgag gacacgccta cgccgtagag ggcgacgtct acttcgacgt cctcagcttt
    28801 ccctcctacg gtcgcctcat cagccagagc ctggacgagt tacaatccgg agcccgcatc
    28861 gacgtggatc cccggaaaaa acaccccctg gacttcgccc tatggaaggc ccaaaagccg
    28921 ggggaaccgt catgggaaag tccctggggg cccggacgcc ccggatggca catcgaatgc
    28981 agcgccatgt cctccaagta ctgtggtgaa accctggata tccatgcggg aggcagtgac
    29041 ctcatcttcc ctcaccacga aaacgagatc gcccaatccg aggccgcctc ggggaaggcc
    29101 tttgtccgct attggatcca caacgccttt ttgctcatcg atgcggagaa aatgtccaaa
    29161 tcgctgggga atttcatgac cgcccgggag gcccgacggc acttctcccc cctcgccatc
    29221 cgcttcttca tgctgggggc ccactaccgc tcccccctaa acttctccga agaggcgctc
    29281 aaacaggcca ccgccgccgt ggagcgcctt cagagctcct ggagggagct ttcggaggcc
    29341 cggaaatccc ggccggaggg ccaaccccag gaggagctcc gccaggccct ggcagcggcg
    29401 cggcgacgct tttgcgaagc cctggacgac gacttcaaca cggcgggagc cctgggggcg
    29461 ctctttgagg ccgtgaaagc ggtgaacagc gccctgaaag ataatcgcga gggggacctc
    29521 gacgccctcc agcaggccga agagctctac cgcctggcgg acgccattct gggagtcttc
    29581 ggcctggacc gccccgagga tcccgccgcc gccgaagatg cggcccagat agacgccctg
    29641 gtggaacggc gcctcctggc caggaaaaac aaggacttcg ccgaggcgga ccgcatccgt
    29701 catgagctgg gatcccgggg gatagtgctt gaagataccc cggggggaac gcgctggcac
    29761 cgcgcccttt aggaggcact aaaagcgggg gcgtccccct tccggggccg cccccgcctt
    29821 aaaaagatct cacggcgccg aagaaacgcc tttcaggagg cgtaacaccc ccgaatccgg
    29881 ggtaaggatg atgggagtgc ccggcaggtt ggttttgtag aaatcgagag ttttaagaaa
    29941 taggtaaagg tctggcgcct cgacggccac gtccgcgatg agctgttggg cctgggcatc
    30001 ccccttgcct ttgacttctt ccgccttgcg ctgggcgctg gccaagatct ccgtgtgctt
    30061 tttctgagcc tcggagcgca ggcgagccgc ctccgacctt ccttcggccc ggagctgggc
    30121 ggcgacccgg cttcgctccg ccaccatgct acggtacacg gcgtcttcgt tttcttctgg
    30181 cagggagacc cttttgaacg ccactcggag gacctccacc ccgtatccct tggtctggtt
    30241 attggtggcg acggtggcct cgaccaaaga ctggtcccgg gtgttgaaga tcaggtcgtc
    30301 gaaggtggcc tttcccgcca cgatgcgcac cgctgcgtag acggagtcgt ccagtcgctg
    30361 ttgagcggcc tccacggtgc gaatggtccg gtaaaagagg tcggggtcgt ggatctgaaa
    30421 gaccgcgatg ctgtcaaaga ccaagttttt cttgtccgaa gtcaccaccg ccacgggggc
    30481 ggcatcatac tccaaaaggc gcttcgggta tcgcaccagg gtgtccatga agggtatctt
    30541 gaagtaaagc cccgcctcct gcacggagcg caccaccttg cccagacgca aaaccacccc
    30601 ctgctggtcc gccgggagga caaaggctcc ggagagcgcc cccgccatca agaccacacc
    30661 caaaactccc agcacgcgac ggcgcaccga tcccttcctc cgggtcatgg cctttctccc
    30721 ccttcctttc cctgggtttc caactgtttt agcagaccgt tgacatccaa catgaggaag
    30781 gactgggccc ccgaggctgc gggggcgtcc aaaatggcca ccttcaaacg gggccagacg
    30841 tctttcatgt tttctaacca cagagtattc ttcaccagcg aggcgttctg ggcgtacatg
    30901 ggggccaggg cccggatgcg ggccacctct ccttgggcca cctgaaccac ccggaagctg
    30961 taggcctctg cctcgttggt gatccgttgg gcctctccct ccgcctgggg gatcagttca
    31021 ttggcaaact gctgggcctc cagttgcagg cgttcctttt ccgcccgggc actattcacc
    31081 tcgctgaaag cgctctgcac cgtcgccgga gggttcacgt cctgaagttg gaccgccacc
    31141 acgcggactc cggcctgcag gtccgtgagc accttctgca catcctggcg cgtctggccc
    31201 tggatcgcct cttttcccgt ggtcagcacc tcgtccatgg ttcgggaggc caagaccttt
    31261 cgcaacaccg actccgccat gtcgcggatc agcttttccg tcgcccggtc ccccaggggc
    31321 atcctagtca cgtaatgcac ggggtcggag atctgaaact ggagcaccca gttaacctcc
    31381 acgatcttgt tgtcgctggt gagcatcttg gactcgacgg gctcgtcccg gaactgggga
    31441 tcttctccgg gaagcaccgt acgataaccg aattcaaacc tcctcagccg ctccgtgttg
    31501 acgatctcca ccgcatccat cccggggatc ttgaagtgca acccctgatt ttccaccgaa
    31561 aggacccgcc ccaggcgaaa gaccaccgcc tgactcccgg cgggcacgat gtacaccccc
    31621 gccaggagcc cccccaaaaa aagcaggaga cctcccaggg ctcccaccca ccgcctccaa
    31681 tgccctcctc ccgggatgtg tacctttctt cgcagagagt cgaagggatt attctcattc
    31741 tggccaaaat ctaccatgat cccacccctt ttcgaggatc gccatccccg gttttttcgc
    31801 cggcgccatg gtattataca tcaagagtca tcgctttcat ggagaggaat caatttttaa
    31861 cttaaaaaac atcacgatag atcggcgctg gggagaaagc tccgtcccgg aggcctcgcc
    31921 cgggagggca ggactttcgc tcctccggtc tgggaggtgg tcgccataaa atctcttgaa
    31981 tcactgcgtc gagcccttcg ttccctaggc agcgctggag tggccctttc aggaggcgtg
    32041 gacagcgctc tcctcgccgt cgtcctgggg aataccccgg aagtccgggg ccacgcccta
    32101 actctggcct ctcccctctt gggacgggag gaggagcgcc gggcccgaga cgtggccctc
    32161 tttgcggggc tcccacacac gctcctggcg tcgaatgagc tggcccttcc cgaggtggcc
    32221 caaaactccc ttaggcgttg cttcgtctgc aaggcccatc gtttggccct tctgacacaa
    32281 tggggcaccg cagcggggtt cgattggatc ctagatggca ccaacgccga cgaccttcag
    32341 gaggaccgcc ccggcttgga ggccctgaga tcctgccccc gggtccgcac ccctctggcg
    32401 gagggagggt ggaacaaaaa tgatgtgcga gagctggctc gaaccctgga gattcccgtg
    32461 tggcgccggg cgagcaatgc ctgcctcgcc actcgcctgc ccttcgacac tcccttgacg
    32521 gcatcgacac tgcgacggat cgacgaggcg gagtttcgac tgcgctccct catccccgag
    32581 gaaatccccc tgcgccttcg cctccagaaa gactcggccc gcatcgaaac gggagaaggg
    32641 gggcgcaccg ccctggccac ccctgcgggg gaagagcttc tggggcaaat ccgagggttt
    32701 ttggaggtct caaatgtctt cgtggacggc caagggtacc gtttcggggg tgcccgtcga
    32761 taacaaacgc ttccatccca gcgaatccat cgggcgaggg ggccgtttcg aaaacaaaag
    32821 gccccgaggt ccgaaagcgc cgtgctaaag gagcttccag acccggggca cgatatccta
    32881 ttcaaacaaa atatctttcc tgaaactagg ttaaaccgac ggagcgcctt cttcctgccc
    32941 ctccgaaaag cgcctatgcg gagcgttctc ctatccctcg ttcctccgga gtgccgggga
    33001 ccagggaatc ccaaaaggcc gcacagagcc ccgccactgc catgggggtc ttgaggatgg
    33061 cccaaaccac ctgtcccacc acgccgaacc cgaagaagag ctcctggttc tcggcgaccc
    33121 accccggtag ccccagggcc atgaggaagg caaagcccac gatgaggacg ttcctctggc
    33181 tccccatgtc cgcccgcatg agcacctgaa tgcccagggc tccgatggtg ccgaagaggg
    33241 caatgtatgc ccctccgatc accggactgg ggatggtggc gatgagggca gagagcttgc
    33301 ccagcatgct catggcgatg aggatcacgg cccccgcacg caccacccaa cgggaggcca
    33361 ctccggtcag gccaatcagc ccgatgtttt ccgtgtacga cgtggtcccc accgccccca
    33421 aaagccccgc caaggcgcag tttgcccctt cggcgccgat cccccggctg atgatctttt
    33481 cgtccggatc cggaagaccc gaaacgtagg aacaggagtg gtaatctccg atggattcaa
    33541 tcatcacaga gaaaaacccc gccagaaggg cccccaccgc cacgatgctg aactgggggg
    33601 gcccccaggg catgagcccg gtgaagcgaa atcccggagc ctccaccacg ttgaccaggg
    33661 acacgtgagc cggatgcccc tcgggaaaga atccccccgc gctccccaga tagcagacca
    33721 gataggccaa caggatggag agcaacaccg aaaaaatatt aaaatacttg tttttgctca
    33781 tgaggccaaa gaaaaacacc ccccccacca ccatgagcga cacgggccaa tagcgggccg
    33841 cgttggactt caccgccacg tcgaataggg agaagccgat ggccataata gtggggccaa
    33901 tcaccaccgg cgtgatgatc cggcggatca tgcccgccag gccactgtac cccaccagcg
    33961 ccaaggcgat gccccccacg atgagggctc ctcccacgtg ctgcatggtc gcatcggggc
    34021 ccagggcctt gtagatgccg atgatggtca tgatcggcgg gataaagctg aagctggagc
    34081 cctggacgat gggtagcccc gtgcccaatc cgggcgtggt ctgaatgagg gtggcgatcc
    34141 ccatggcgag gtacacacag gagataaact tcgccacctg ggccacatcc atgcccatgg
    34201 cgggaccaaa gatcaggggc accaacgtgg tggccccgaa gagcgtaagg acgtgttggg
    34261 ctccagccaa caacaaaatg ggccccggcg gcctgtcgag caacccgtag acgatctgct
    34321 ttctcgccat actaaaaccc ccttcgcgta ataaggactt cctcaccgcc cccacgccgc
    34381 caaaaaaaaa ggccaactct ccctttgatg gggagagcgg ccctacccgc cctttatcca
    34441 acgctccggc tcatcatatc ccatggcgat tctcccttcc gggcctcgcg gaacccgatt
    34501 aaaggtgcaa aaatccttcg agaacttatt ttacgacggc cctgccgaaa gggcaatccc
    34561 ccactcccca tctccttcac tcctccaagg acttcactcc cccgtacatc tatccctcct
    34621 ccgctccggg agacccgttc tcgagccccc cctgaagcgg cggggcttta ccccgtcgac
    34681 ctcgcctctc ctgaaggaaa tgatccctcg ccccatcttg acggaacggg gaaaagcggt
    34741 tacaagagcc ttggagtttt ctccgggctc catcgcccaa gacccggggc gtcgcttcaa
    34801 atacttctcg ataggggcga tcccatgatc cgtctgacca acattaccct tcgcttcgga
    34861 gagcgcctct tgttcgaaaa cctttctttt ttcgcttcgg acagcgcccg catagccctg
    34921 gtgggggcca acggcgaggg gaaaaccacg ctctttcgtg tcatcctgaa ggaacagatc
    34981 cccgacgcgg gtagcgtgga gctctcccgt tgccagaaca tcggccatct gccacaggat
    35041 gtggcggagt tgggcagcgg caccgtgctc gacttcctca aggagcgggc cggagtggcc
    35101 gccttggaag aagacctccg gagcttggag caccgcatcg ccgccacagc caccgccgac
    35161 ggggagtacc gccgcctcct atccatgcac gagaatgtgg ccgccctttt tctcctgaag
    35221 gatggctact ccttcgaagc caaggcaaaa aaagtgctcc ggggacttgg ttttcgcccc
    35281 aacgccgccg agactccctg cgcggtcttt tccgggggtt ggaagatgcg catcgccctg
    35341 gcggcgattc tcctggcccg cccccccgtc ctcctcctgg acgagcccac gaaccacctg
    35401 gacacggaga gcatggagtg gctggaagga tacctcaaag actatgatgg atgcctcatc
    35461 accatatccc acgaccgaag gtttttggac aagatgacta cgcagacggc ggagctgagc
    35521 cggggcacca taacactcta ccgagggaac tacacttggt atctcgccga acgggaaaag
    35581 cgccgggagc agctaatcaa agagatggag ctccagcgct cggaaatcaa gaaaacgcag
    35641 gaattcatcg aaagatttcg gtacaaggcc agcaaggcat cccaggtcca gagccggatc
    35701 aagagcctgg agcgcttcca ggaggtccgc ctggagagcg accacaaaac ggtgaagttc
    35761 cattttcctc cctgcccccg gagcgggcgc accgtcctga cggctcaaga tctgggcatg
    35821 gcctacgggg ggaaagaggt cttccgcagt ctgaacctcg tgctggaacg gggggatcgg
    35881 gtggccctgg tgggagtcaa cggcgcgggc aagtcgaccc tttgccgtct tttgagcgga
    35941 gaggagcccc ctcaggaagg ggacgtccac ctggggtttg acgtcaagcg ggcctttttc
    36001 tcccaggaga gccctcgaaa tctgaattac caaaacaccg tgtggcaaga ggtccaaggt
    36061 ctgggtaccc ggcatgacga cctggccaag cgaaacctcc tgggggcttt tttgttctcc
    36121 ggagacgaca tccacaagcc cgtcgccgtg ctgtccggcg gggaaaaggc gcgcctggga
    36181 cttttgaagc ttctgctgga ggacacgaac ttcctcatct tggacgagcc caccaatcac
    36241 ctggacatgg ccaccaagga catgttccag caggccctcc tatcctacgg cggcaccttg
    36301 ctcctggtgt cccacgaccg ggcgttcctc gacgacctgg tccatcgcgt ggtggaaatc
    36361 cgcgacggga aactctttga gtatcgagga aactattctt ttttcctgga gaaacgggcc
    36421 gagaaccttc ttgccgaagg ggcggaggga tccgaggatc ccccctcctt ccccatcgct
    36481 tcgccggaga aggacttctc cggacgaaag agccgggagc aaaagcgaga ggacgccgca
    36541 tatcgggccc gtctctccgt cctgcggcgc gccgtgatcc aagccctgga tcccgtggaa
    36601 aaaaggatcg ccgccctgga gggagagaaa aaatctctgg agcgctccct ctgcgacccc
    36661 gccacgctgg ggaattccgg cgcggtgcag gaggccctac agaggctggg gaccatcgaa
    36721 aaagagctca acaatctcat gccccgctgg gaggagctca tggcggagat ggaagcggtg
    36781 acacaagaat ccccttaggg gcccctgcaa tccgaagggg aacgacgatc tcgaagggtg
    36841 aggcgacacg acttcggcgg ccccgctcag tgtcatttcc cgctcctcca ctcgcagcgt
    36901 ccctttaggg cgcagcggag gcacttatcc catcgccctt cgctcccctc cttcgtcccc
    36961 tccccgggga tcaccacggc ggcggcggtg agggacatca aggggaagag aacttggtgc
    37021 ccgttcaacc ccatccccac ggatccgagg tccaacagat cccccagggc tcgctgcccc
    37081 tccaggggcc aggaggggcc ttctccggga cttaggggga gagtcagggt ctccccttga
    37141 gctcgaaggt cggcccctat cctttcccaa gccctacgcc ccgcagcctc cagccatcgc
    37201 ccccccacca cggccagggc gtagcgccgc aggaggtctc cctccggagc cccgccgtcg
    37261 acgccttcca aggcccccag ggtgagggcc aagaatatgg ccgactcccc tccttcccaa
    37321 ccctcgggct cccccacgat gtgaaggggt atgggttccc ctggagacct catcgccacc
    37381 cacccctctc cctcgcgccc cagggggcag cgacgccaca gggcccgggg gcgggcgcta
    37441 cgcccaatcc attgcctcgc ccattccatc gcggagacaa agggctcccg atggagatcg
    37501 gggccccacc catcctccgg gatcccttcg tccgccatca gagggataag ttctttatga
    37561 tgctcccaga aaaaatcccc caaggtctta ttccctccga ggaaaggcca tggcgcctaa
    37621 aaagagggac tggaactctg gggtctctgc cagggacacc actttagccc tgaaggccac
    37681 atcccgggcc cggcgctccg ccggagctcc ctccaggagg acgcgcaggg cccctccccc
    37741 cgccgcgtct tccagcgcca caaaggatcc atcaaacccc ggcggcacga cccccaactc
    37801 tcgcaccgcc tccgggtcta gctccgctcc gaaggccccc gcaaagacac actcctccac
    37861 cgcctccgcc ttcactcctc cttcggccaa caggcgacag atgcccactt gaagggccgc
    37921 tttggccaac tgcacctctc ggatatcgcg ctgccagatc gtcacctctc cttcatcgcc
    37981 ggaggccaac attatccccc tctcctcccc ccgcattctc cgccggtccc ccccgtctcc
    38041 tggaaagggg tcaccccgaa ggcgaccgcc gtcgtctaaa actcccgccc ggcggagggc
    38101 tgccaccgca tcgatcaggc ccgagccgca gatcccccgg ggaggggcgg aatccaccgt
    38161 ctccgccctt agatctccgt cgtggagtcg cacgcgtcgt accgccccgg ggcgggcgga
    38221 catgccctgg tatatgtgcc ccccttcaaa ggcaggcccc gccgccgccg aggtaaccca
    38281 aaggtcctcc tctgaaacca aggcgatctc tccgttggtg ccaaagtcca ccaccaggcg
    38341 agttctcttc ggaggcaagg tttctacggc caacaagacc cccaccaggt cggcccccac
    38401 aaaggccccc accggaggaa gggggcgcac cgtcgcgtcc cggataaact ccatccccaa
    38461 acatcccgcc gaaagggcct cgggcaaggg atccacgcag acaaagggct cccgggccat
    38521 cgttccgggg aggtagccca aaaagaagtg ctccatggga gggtttccca ccactaccat
    38581 ctccagcact tcctcgccgt ctcccccgat ggaggctagc ccctctttca ccagcgattc
    38641 caccgcccgg cgcaacgcga cgcaaagctc gtcgaagggg cccaaaccct ccaacgccgc
    38701 ccccatccgg gaaaccacgt cggcaccgaa aggcgcctgg ggatttgaag aggcgccgga
    38761 ccacaatact cttcccgtga ggagatcggc caggtagacc gccaccgtgg tggttcccag
    38821 gtcgcaggcg actcccagag gagtccgccc cggggacagg cacccccgga ggacgccatc
    38881 tcgcaccggg agaataaagg ctccaggctc cccggggcgc caccgccggg aggcctggag
    38941 cagcgcggcc cgggaaacat gaccgacctc ctccccttga tgggccagcg cccggaccag
    39001 cgcctcggaa aggcttcccg cttccccgag ctcgtcccag ggaatgagta gctccggagc
    39061 cctccagtga gtcctttgcc ccccgctcga tcctttttcg ccccccgatt ttcccggacc
    39121 caccgtaaga ttcacccggg cccccccggt aagacgggct cggcacgcca gacgatatcc
    39181 ctggctcctt ccctcctctc cgaggagctc tttttccccc tcctccggcg gggacacccc
    39241 tccggacagg cgcacccggc atcccccgca tctcccctgg cctccgcagg gagcttcgaa
    39301 ggcgaccccc agggaccgca gggcatgcca caccgtgtgt ccctcgtacc ctgcggggat
    39361 cgtcacggag ccttcggcgc cctcgaaggc ccccggggtc ccctcttgcc cgtccttcgc
    39421 tttgctggcc atccacaccc ctccccttcg ccccaccgcc ccaagagctg gcgggggacc
    39481 ttcttttagc cccctagata accgatccgg gggagcgcca tttctccctt gacctggcgg
    39541 ggccttctga tactatactt tacggcataa aatctagacc ctccaggaaa agatcctcca
    39601 gaaagggagt gttttcatga tcctcataga tgctcgggga aaaccctgcc ctcaaccggt
    39661 gatgatggcc aaggccgccg tggaccaagg ggccttggag attgaggtgc acgtggataa
    39721 cgacatctcg gcccagaacg tcctccgatt cctcggaagc caggggttta cctcgtatcg
    39781 ctccggcgcg gcgaccgata tcgtggtctc gggtcaccgg gaaggaggct ccaccgggag
    39841 ccaaagggcc cccgaggtcc cgatccccgt ctcccgctct ccggaaggaa tcggggtgct
    39901 ttttctgggg cgcaccctgg ggcaggagag tcccgagctg ggggaggccc ttatcaaggc
    39961 ctttctcggc accttggcgg cctcttcccc cccacccgcc acagtggccc tcatgaacgg
    40021 ggggattttt ttggcgctgg aggagagttc cacttcggag gtcctgcggg agatggaatc
    40081 ccgggggtgt cgcgtcctgg tctgcggcac ctgcgtgacg cacttcggcg tcgccgatcg
    40141 attggcagcg gggaccctat cgaacatgtt cgagatcacc gacgcgatgc tccgagcccg
    40201 taagaccctc gtcttaggtt aggggagctc cccgtgtact tcaacaacgc cgccaccacg
    40261 tggcccaaac ctcccgccgt ggctgaaaag atgagggatt ttttgacccg ggggggggcc
    40321 aatctcggcc gaggaagcat ttcctctcgc gatagggcca ccctggatct ggtctccaca
    40381 tgtcgagagc gccttgcgac catgctggaa ggacacgaag ggcgcgaccc ccggtacgtc
    40441 accttcaccg acaacgtcac cgcatcgctg aacgtggtct tcaagggatt tctccggccg
    40501 ggcatgcggg tcctcaccac gtccatggaa cacaacgccg tggtccgtcc cctccgctcc
    40561 ctggaggaat cggtgggcat cgaggtggtg atcttaccct gcgacccccg ggggcacctc
    40621 gaccccgggg acctggaggc cgccctggcc tccgggggag acctcgtggc cctctcccac
    40681 gcgagcaacg tctgcggcac catccaggac atggacgcca tcgcctccgt ctgccaccag
    40741 cgccgggttc ccctggtggt agacgtggcc cagacggcgg gggtcctccc cgtgtccgcc
    40801 gaacgatggc acgccgccgc cgtgtgtttc accgggcaca aggggctcct gggaccccag
    40861 gggatcgggg gcatcgtctg gcacccggag ttcgcgcacc ggacccgtcc cttggtggag
    40921 gggggcacgg gaagtttttc ccacgaggaa cggcagccct ccgtgttgcc cgacaaattc
    40981 gaggcgggga cccccaatct gcccggcata gcggggctgg agggcgccct ggcttggatc
    41041 gaatcccagg ggagggaggc catcgcccgg cgagaacacc tcctggggga gcggctcctt
    41101 aaagggctcc gggccattcc ccgggcgaca ctttacggca tggcctccat ggaagggcgc
    41161 ctccccgtat tccccctgaa cgtggagggg tgggacaacg gtacgctggc cttcgaacta
    41221 tcggaacgat ttggtctcga gggccgcccg gggctccact gcgccccctg ggcccaccgc
    41281 accctgggga gctttcccca gggggccctg cgcctttccc cggggtactt caccacggag
    41341 gacgaggtgg accgatgcct ggaggccctg gccgtcctct cccgggagcc ttcccccgtc
    41401 tgagctttca ggagagggcc tgatctagtc cgcctccccc tccgccccca ggggcacgat
    41461 acaccgcacc agggttccct ttccggggga agagcgcagc tccagcgtgc cccccacaag
    41521 ggccatgcgc tcctgcatgt tggccagtcc ccgatgcccc cgaacccgca gggcgttaaa
    41581 atcttccccc ggggagaacc cctcgccgtc gtcctctatc tccaaggcga agaaattccc
    41641 ttccctggtt ccccggagct ccacccgacg ggccctaccg tggcgcacgg cgttatggat
    41701 cgcctcctgc gccacccgaa aaagggacag caccgcttcg gaagataggt cgtccgcagc
    41761 agattcgcac tccacagaaa tcgccacgtc gaggtgggcc gaaaggcgct ccgccaactc
    41821 cgtcaccgcc tgggaaaggc ccagatcaag ccaaggaggg gagagttcgt cgcagagccc
    41881 ccggagctcc cggaccgcca gatgggcccc ctcttccgcc atggtcatcc tcctgctccg
    41941 gtcttcgatt gatggcgcca ggtcggcgag gcggagctgc tggatcaacg ccgtgatgct
    42001 ctgtaggggg ccgtcgtgga tctcccgcgc caatcgagtg cgttcttgct cctggacccg
    42061 caccaggtcc gtcacggaac ggcgcatgag ccgggatttt tcgatggcgg tctgggcgag
    42121 atggcgcaag actccccgca ggcgctggag ctcgaacacc gctggggccc ccttggctgc
    42181 gggggtctcc tccccccatc gcagccggga tacctcattt tccagggccc tcaggggagc
    42241 gatgagccag cgccacaggg cccacaccgc cgcgagcccc gccagggcaa agccgctgat
    42301 caccagggcc cagaggctcc catagcgcac catgggcccc aataaggcct cccacgacac
    42361 cgccgccacc acaaaccacc gggtatcgcg cacggggtac gccgcgatgg tgtacttctt
    42421 cccgtggatg tctttgatgt ccaggacatg ccccacaggc acccccccgg gggcaatctg
    42481 ggccagcgat tcggcccccg gagaggcggt catgatctgt cccccctcgt caatgaccgc
    42541 cacccatccc ggcatggaaa gacgccagga gaagaagcga aagaggaact cccgcgagga
    42601 atccagggtc cggcggtccg ggtattcctg cccaggaagg ccaaaacgcc cccagcgcag
    42661 gtccaggcga gaggccacca tctccgccag atcctgaacg taggagcggg ccaccacccg
    42721 gatggcgcgc tcgtgctcga tgaggctaac cccggaggcc accaagatgg ccaacgacgg
    42781 caggaggatc gccacggtga gggccaggag gagatgcctt ctcaatccag caagtcctcc
    42841 aacgtgacga tgccgtactt aagggccaag agcatggcct cggtcttgtt tttggccccc
    42901 aatttatcgt aaatcgaggc gaggtgggtc tgcaccgtgc ggtcgctgat aaagagctcc
    42961 ctggcggcct ccttgctaga aagtcccttg gcggccaaaa ggagcacctc ccgctctcgc
    43021 acagagagct cctcggggga aaagtccccc tcgatgtttc gggaaacctc ccggtccaga
    43081 tagactcccc cgacggagac gacccggatg gcggacgcca gctcttccgg ggtggccgtc
    43141 ttgagcacat accccttggc cccgttgcgc aaggaggcca ccacatattg ctgagcgtcg
    43201 taggaagtca gcatgagggc cgccgtgcgc agcccttctt ctttcatgcg ccgcaccacg
    43261 gagactccgt cgcccttggg catgcggata tccatgagcg ccacgtcggg ggccagggcc
    43321 cgaatgcgct ccagggcctc ctccccgtcg gaggcctctc ccaccaattc catgtccttt
    43381 tcctgctcca ggtacgccgc gatccccgat cgggtcaggg ggtggtcgtc ggcaagaaac
    43441 accctgatca tgggcttacc tccttcgtgg tatcggcccg aaggcccggg cacgacaccc
    43501 cttgtccgaa agcacgaaaa agggaatcga tgccacctcg ccccattctc tcgcaagggg
    43561 aggctctccg tccataggtc aaaaggcgca ccctgggacc ttcacctccc cgggacaaaa
    43621 aatcccccag ggcccaggcc ctgggggata aaacaccgaa ggaaggggga taacggaggg
    43681 ttagttgcgg cctatggtgg ccaccatcac ggccttgatg gtgtgcatgc ggttttcggc
    43741 ctcgtcgaag accaccgagt gacggctccg gaaaacctca tccgtcactt ctcggatgtc
    43801 gtaccccttt tctttcatgt ccttggccag cttcgtctcg aagttgtgga acgccgggag
    43861 gcagtgcagg aagatcacat ccgggttgcc cgttttttcc atcatttcca tggtgacctt
    43921 gtaatccgtc agcaacttca ccctttcggg gattttgtcc tcttctccca tggaggccca
    43981 cacgtcggtg tatatgacgt cggcgccctt gacggattcc aagctgtcgc tgacctcgat
    44041 gaccgccccg gtgatcttgg cctcttctcg caccttggcg aggatctcgg cgttggggct
    44101 cagctccttg gggcccaatc ccacgaagtg catccccgtc ttggcggcgc cgtacatgag
    44161 ggcgtaggcc atgttgttcc tggtgtctcc ggcaaagacc aactttactt tgtttagggg
    44221 tttggccacg tgctcttcaa tggtcatgaa gtccgccagg atctgagtcg ggtggtctat
    44281 gtcggtgagg ccgttccaca ccggcactcc tgcatacttg gcgaggagct ccaccacttc
    44341 ctgctggtag ccccggtact caatgccgtc gtaaaagcgc cccagtacct tagccgtgtc
    44401 ctccagggat tccttttttc ccatctggga gctgctggaa tccaaaaacg tcacgtgggc
    44461 cccttcgtcc atcgccgcca cttcgaaggc gcaacgcgtc cgagtggaag ttttctcgaa
    44521 aagcaagaca atgtttctcc ccgccaacag cggctcccgc acccccgtac gcttctttgc
    44581 cttgaggtcg tggctcaggt ccaacatgta tcgaacctct tcgggggtga aatccatcag
    44641 agtcaaaaaa ctacgtccct tcaggttaac cggcatgata ctctctcctc ctttgacatc
    44701 acgaagccct ggcttcgtct actgctcccc acggcccgga cgattcctcg aacggggacg
    44761 aaaaaaaaga cggcgattac aactgttctc tcatcagggg gtgggtcata caacggggcc
    44821 ccccgcgccc ccggcccagt tcggctccgc ggatttcgaa aaccttcact ccgttttccc
    44881 gcagcacctg attcgacacc acgttctggc gataggtcac caccactccc ggcgcgatgg
    44941 ccaacgtgtt ggtgccgtcg ttccactgct cccgggccgc cgcgatggga tcccctcccc
    45001 ccgtctcaat gaagcggggg tggtccatgt gcaacgtacg ccggaggcaa tccgacagac
    45061 tgtgatggtc cacaatgccc tccagggcgc cgtcggcgtc atagcgcagc tcggcgatgt
    45121 gcaagtgctc ttccagtccc ggatagatgg tgaaagcgtc ccgatccacc atggtgaaca
    45181 cggtgtccag gtgcatggag gcccggattt tcgggatatc caccgccaaa acggtgcgga
    45241 tgcccagttt ctgggccatc ctccgcccca cgatctggac ggtgcccgcc cgggtgcgct
    45301 gactcacccc gatggccacc gtgtcctcgg agagcaccag cacgtcgccc ccctcgatgg
    45361 tgtagggagc gcaatcccgg gggtcgtccc caaagatgat ctggacatcg gcaaaatagg
    45421 ggtggtggcg gaaaatggcg tgggcgtaga ggggctcccg ccgccgggca tcaaagttca
    45481 tgcagcttac aatgactcca tttttcacga aggcgaaggg gtctctctgg aagtacaggt
    45541 tgggaagggg gcggatgaga aaatcgttga catccgggaa gcggatcgcc aggctccgga
    45601 tgtcccccac gatggcttcc gtctctttct tcgtgagacc cgagatgagc acctcggcca
    45661 ggtctgcggg gttcatgccc atgagctctt tttttagagc ccccgccagg ggaatgtcca
    45721 gggcctccag ggccaacgcg tcgtccagga gctcgtcgcg caggggttcc tcccggagga
    45781 tgtgggccag acaattgcgg aaataaacca cttccacgcc attgtcccgg agcaggtcgg
    45841 cgaaggcgtc gtgctccctc tgggcctctt cgagccacag gatgtcgtca aaaagaagct
    45901 catccttgtt gtgaatggtg aggcggtcca gttccagtcc tggccggtgg agcatcactc
    45961 gcctcaggcg ccccacctcg gaacccacgg aaaaacctcg gctatcgctt gtcatgaccc
    46021 cactcctctt cgggaagaaa tgaaccaaaa aagcaagccg gtttactctt tcagttatat
    46081 catagccctt ccatcctggc aagggtcatc cctttaccct tttcgtggcc ctcccctccg
    46141 accccgggcc gccgcccccg tcgcctactc gccgatctgt tcctgcatgt aggggggaag
    46201 cacaaaagag gcgccgtgaa tggcctcgga gtagtacctt gttccctggg gcacggggct
    46261 tcggaggatg cgagggtcgt ggcgacggct tcccaccatg taggtccagg tccccgtagg
    46321 ataggtgggc atggagcccc agcagagatg gaccaagggg aagacgctac gcatctgctg
    46381 aaaggtgggg gtgaccatgc ggggttctgc aaagggagat tcggtctggg ccatcaacat
    46441 cccatcttct ttcagagcct ggtacacgtc ccggtagaag ggggcgcaaa aaaggcccgc
    46501 cgcgaagtcc acgggatccg tgctatccac gatgatcacg tcaaagctcg cctgatggtc
    46561 tttcacaaac accaaggcgt cctcgtggag tacggtgacc cggggatcat ccagcgcgca
    46621 gctcaccgag gggaagaact cccgggaagc cctggtcact tcggcgtcga tgtctaccag
    46681 aaagatctgg gacacggccc cgtgcctggc cacctcccgc acgacgcctc cgtcaccccc
    46741 ccccaccacc agcactcggc acggggcagg gtgggcgcaa agaggcacgt gggccatcat
    46801 ctcatggtag gtgaactcgt ctttttcggt gatctggatg gccccgtcca gcaccaacgc
    46861 ccggccccac tcttccgtct ccaccaccaa cagtcgctgg tatggggtct cttcgtgtcg
    46921 cagtacttgc ttgatgcgca accccatcct catgtcaggg gtctgttctt ccgtaaacca
    46981 caggtcgttc gtcttgcgct ccatcacctg catcgaaagc actcccttct cccgcgtcct
    47041 cgatccatct ccccttcgcc tccgtccttc cgtcgcggtg gcccgctttt tcgtaaagtt
    47101 cctgggatca tcggagatcg cgccttaaga ggctccttcg ttccctaacc caccgaagaa
    47161 gcatgccgcc cccaccgtag cggcctcttc ttcccgggcc tcggcctcct ctcccgccag
    47221 gtacaaggtc tccacggcga ggtcgtcttt ttcgtcgact tcccggaaaa ggatctcttc
    47281 cacccgcatc agcgcctcct cggcccctcc gagacaagtc accaccgccg cccgacggga
    47341 accatccgcc aggcacagca cccccaccgc cgcccctacc accgactccg agagaggggg
    47401 ggcaacgatg gcggcggcga ggctctgaac gggaggcggc ccttcggtcc atgccattcc
    47461 ttccagggtc gatgctccgg cgggcaaaag ggcctctcgc acggcaaatc catcgtaacg
    47521 aggggcctca ggaaaggcct caaggtcttt ccccgtgacc aagacaaacc aaggcctccg
    47581 gaggcccgtt tcctccgaag ccaccccttc gagatcagga gtcttggagg ggaaagtctg
    47641 gcttttctct gaatccatgg gggttcctct ccctctttca gatctgcggc gcctacgctc
    47701 catctccggg ggctccgccc tcctccgggg tgctgtctct cgaaggatcc gccagagctc
    47761 ggcgcagggc ctccaccacg tcctccggat tttcccgggg cagctcgccc agaaactctc
    47821 cccggcgaaa gaggatcact cctccgggag atccggccac ccccacatcc gcctcccggg
    47881 cttcccgggg cccattaact tcgcacccca taacagcgat ggtcactccg tcgggaaagg
    47941 agtccaggtg ggggcgaagg gcggcggcga tggcgctcac atcggccctt cggcggccgc
    48001 aggtgggaca agacaggagc tgggccccca gagcccggag ctctagggca cgcaaaaggg
    48061 cgtatcccac gcggatttcg tcgggcgagg gccccgtgag gctcacccgc agcgtgtctc
    48121 ccaacccctg agccaggagg aggccaatac cacaggctcc ccgcacgatc ccctccacgt
    48181 cgccccccgc ttcggtgatg cccacgtgca gcggatagtc cgggtgacgc tgccccaaaa
    48241 ggacgttggc ccgtacggtc tccggcaccg acgtggcctt ggccgaaagg atgatgcgag
    48301 aaaatccctc tcgttccagc gccgccagct gatcttccgc agcgtgaagc agggccaagg
    48361 ctcggtctcc ctccgcccgg gccatctggt cgttgctgag cgatcctccg ttggcgccaa
    48421 tgcgcagcgc ggccccccga tcttccgccg ccaccaccac ctcgcgaagg tcgcgctgag
    48481 acatgttccc cgggttcagt cgcacggcgg ggcacccctg cctcagggcc tccaccgcca
    48541 gggccggatc aaaatggata tccgccatga gaggcatggg gctcttgcga cagagctcac
    48601 ccagggctcc cgccagctct cgacggggaa aggccacccg caccagctcg catcgggccc
    48661 gatccaagct tcgaagctcc ccttcgcacc ccgcgaggtc ttccaagggg gttttgagca
    48721 tgctttccac tctgacgggg ttatcccctc caatgcccaa agcccccaca cacaccgccc
    48781 gcgcacaggc catacccatc gccaccttta ctgcggccta ggactttctt ccggggtccc
    48841 tctcggggcc cgaaccccgc tctaccccaa gagtttgacg aggtcctgcc acgtgatcac
    48901 caggataagc gccatgagca ccgcgaatcc ccccaggtgg atgtactgct cccacctttc
    48961 gggtaaccgt cgcccggtga tcatctcgcc gaagacaaaa aagagccttc ctccgtcgag
    49021 ggccggaaag gggaaaaggt tcaaaagccc cagatggagg tttatcaccg ctaaaaacga
    49081 caaaaaggac cacatcccct ggcgagccgc ctcccccgcc aggttggcaa tgcccacggg
    49141 acccgtgacg tcgatgtctc ggcgagcgaa aatccaagcc accatccccg acaggatctc
    49201 ccggctcatc tgccaggtat agctgaaaga atgccccagg gccgccgcga agccataggt
    49261 gacccatcgg gggcgcacgc cgaaaagggg cgtcccctgc cgggcgtctc ggctcagttc
    49321 gccggaaaag accctcaccg ccccccggtg ctccacctcc agcatcacgg cgccctgggc
    49381 tcccttttcc cggaggcgcc gagacatgtc atcccacgtc gtcaccgcct cgccgttcac
    49441 cgacaagatt cggtcccccg agaccacgcc ccactgatcc gccgggtatc ccgccatgac
    49501 ttcccccacc acggtgctct ccagatctaa gacgccgtgt cccatgagga gggccgccgt
    49561 gagccccacc gccagcagga tgttgagccc cgcgccgctc gccaggatga cgcaccgctc
    49621 ccaggggcgc ttgtgaccga aggattcttc gtcggcacag gactctccct cttgctcctc
    49681 gcccatgccc gccaaccgga cgaagccccc cacgggaaag gcccggaagg accaccgcgt
    49741 gcaccctcgc tcccgggaca ggaggatggg gcccatgccg aaggcgaact catgcacccg
    49801 gaccccgatg aggcgagcac tggcgaagtg cccaaattcg tggatcagca cacacacggc
    49861 gatgacgagg aaaaaggcca aaaaagtcat cgacgaaacc gtccttcccc catcatcgcc
    49921 tcagcgcgtc tctgcccctc ttccaccgcc tccagggcgt ctcctaggga agcgggcgcc
    49981 gcgcccttcc atccctccag ggtctcttcc accaccgagg ccacgtccaa aaaccctatg
    50041 cgcccctcga gaaaggcccg caccgccgcc tcgtccgctc ccaccaagag cgcggggtag
    50101 gcccctcccc ggcgccccgc ctcgagggcc agggaaaagc aggggaagcg cccctcgtcc
    50161 ggggcgtcca gggccagatt catgggagtg gggggagggg gggccagctc ggtctccatg
    50221 atcggcaacc gctcggggaa ggccatggcc acccccgcag ggatgcgcat gtccggagtg
    50281 gacagtacca tcttgctgca tccatctcgg aaacgcacca ccccgtgaac cagggatccg
    50341 gggtggacca gggcgtgaac ccgatgcacg ggaagattaa aaagctgcat cgcctccagg
    50401 acctcgatcc ccttgttcat taaggttgcg ctgtccaccg tgatcttggc gcccatggcc
    50461 cacgtggggt gacggagggc ctgctctgga gtcgctcggg ccatttcctc ccgagaaaaa
    50521 ttccgaaagg gccccccgga ggcggtgagc cagatccggt ccacatcctc ctccttttcc
    50581 ccgaaaaggc actgccaaat cgcgttgtgt tcgctgtcca ccggcctcag ttgatccttc
    50641 cgggaaacca agggcaaaat ccacggcccc gccaccacca cgctctcttt gttggccaag
    50701 gacacgtcgt ccccctggac cagggccctt tccaaagccc ccagagcccc cgttccggag
    50761 gacgcgaaga cccagtgtac cccggggagc tcggccacca tcctatccag ggcccctgct
    50821 ccgccgaaga cccgcccttc cccgccgagg cgagacgcca gctccgccgc cgccccctct
    50881 tccgccagga ccaccacttc gggattgaaa cggcgtacca gagattccat ggcggcgacg
    50941 tttctctggg ccgccagggc ccgaacccgg aatctatcgg ggaatttttc gcaaatccac
    51001 agagccgccc cccccacgct accggtggcg ccgatgatgc ccacgtcttt gggcgttcgc
    51061 gtcatgacca tatcaccccg aaaagaagat aaactgccga cacgctgaat aagaggctat
    51121 cgaagcgatc gagaagcccc ccgtgccccg gcaggatttt gccgctatct ttgaccagag
    51181 cttctcgctt gagcgccgat tccgccaggt ctcccacctg tccccaggta ccgcagatga
    51241 ggccgatgat cagaaggggc atggggggcg ccgccagggc ataggccacc accccgctgg
    51301 cccccagagc tcctaaaagg cctcccaggg ctccttccca ggtcttttta gggcttatat
    51361 cggggcaaag aggcgttcga ccccagacaa ggcccaccag ataggccaag acatcgcagc
    51421 tccacgtgca tagaaaaagc gaaaacagga ccatctgtcc ccagggttcc gtacgcatat
    51481 ccaagaggaa gctccaaggc agggcgacat agatcacccc tcccaccgtt ccccccatgt
    51541 taaagatggc ctggctcgtt cccgtgagtt gtcttcgaaa gacctctatt cccagggtcc
    51601 ccagggccac cagggtcaaa atcaacaggt ccaccctctc gggcacctca aaaaccgacg
    51661 ccgccaggat tcccaagcct ccgagcaacc cgatacctga ggagacatga aagcggcgcc
    51721 gcaagatgcc gtaaaattcc cccagggata tcagggccag gagggatacc acggcgatcc
    51781 acccataccc ccccgtcaag agtcccccca ccaccgctcc caccacgaga cccccggaaa
    51841 gggcccggac caggaggacc cgtcccccca ccccgcgctt gggagatggc agaggagacc
    51901 tcggaagttt tttcgtcccc gccgagatcc ccgggtcccg ggaatcgtcc tgggaggctc
    51961 gagtttcgac ggaaggggct tctttttctt cgctgatcat gggatctcta tccatttttt
    52021 tcggatcctc cgactccgcc atagcgacgt tccctgccgg agaagatttt cagggctttt
    52081 tcgagctcct ctcggccaaa gtcgggccag agagtgtccg tgaagtaaag ttcgctgtag
    52141 gctccttccc agagccagaa attgctcatg cgatgttctc cgctggtgcg tatgactaag
    52201 tcgggatcgg ggaggtcggg gatgtagaac tgggaccgga ggagttcttc cgtcacggac
    52261 cccgagtgcc cggaggccaa aagggcattg actgcgtcga ggatctcccg acgccccccg
    52321 tagttgaggc acaccaccac gtcgatggcc cggcaggacg ccgtggcatt ttcggcgtct
    52381 tcaagcgtcc gtatgacatc ttcgggaagt ccttcccggc tcccggcgaa gcgcaaccgg
    52441 gccccctctt cgcacagggc cttcaccttc atcgccacgt agtaccggaa aagacccatg
    52501 agccccagga cctccgcctt agggcgcttc cagttttccg tagaaaaggc gtagagtgat
    52561 atcacgggaa cgcgcaaatc cttggcggcc cggaccgtgc gctccaccgc ttgcactccg
    52621 gcgtggtggc ccatgatgcg gggcaagtgt cgcgccttgg cccatcgtcc gttgccatcc
    52681 atgatgatgg ccacgtggcg cgccccccct gcggcgctct cttcttgcca cactacgaca
    52741 tccctccttt ggggatctcc gaaaggctcc cccgaggtcc ccacgcgtcc catcgcttgc
    52801 ggacttcctg aatgtccaac caggtttggt gcttcgggct acccaccgtg gaggattggt
    52861 tcctcaagag atagctgggg tgaaacatgg gcatgacgtc aatgcccctc caggaaaacc
    52921 atttcccccg aatcttggat atgccctccg atgtcttgag gatccacttc gccggggtac
    52981 tgccgaggca cacgaggata cgggggttta ggagaaggat ctgagcctgg agaaagcggt
    53041 cgcaggccag catttcttcc atctgggggg cccggttatt cggggggcgg cactttacaa
    53101 tgttcgtgat atatacttcc tggcgcccga tgcccgccgc ctccaagatc ttggtgagca
    53161 gctgccccgc agcccccaca aaggggatcc cttgggcatc ctcttccgcc cccggtcctt
    53221 cgcccaccaa gagcaaagac gaccggcgat tcccatcgcc caagaccacc cgatgcctcc
    53281 ccgctcccaa gggacaccgg gcacaggatt cggcctcccg ttccagggct tcccacatgc
    53341 ggccccgctc ttttgccact tccagtcctt cgggatcgta aagaaggtcc atcacaatac
    53401 gacctcgctc accacgacgt ccaccttccc cacctcgagt cccgtaaaat acgccacgct
    53461 ggaagagaga cgcttctgca aaaacagggc caagtccagc atggtcatct ctcccacaga
    53521 taccgcgatg gtaatggaaa aacgaagccg atccccctgg gaatccacgc ggacttcttt
    53581 cacggaggcc acctggggcg ttctctggga aatctcccgg atgatttggg ccagggcctc
    53641 cggctcgatg gccaagttcc cgagaaacgt gaaggggggc cggacaatgg tcttttcccc
    53701 ttggggctcc tctccgctcc acaagatgcg aagccttccc accatcttgc cagcaaagtt
    53761 cttccgtacc tggagagaag acacggggat gacatgttgc ttcttgtgaa tgcgctcatt
    53821 ccgggcccgg gcaatctcct cgggggacgc cacgtctcga atatgaacga aggcgcgagg
    53881 cggggggatt cccaggcgcc gggagatgcg gcgggccatc tcttccgatg tggccactag
    53941 catgacccga caaggagccg atgccgccag ggcccggcgc acttggaggc gatgcgcggg
    54001 aaactggaag agggctcggc gaatggcaca gatctggttg cgctccgtct tggcgctccg
    54061 gccgcagaca atctgccctc gatggattaa aagaccatca tcgatgagga agtccgtatc
    54121 atgttcccct gccacaagct gggcccgctg gctcttcccg gtcccagcgg gccccacaaa
    54181 ggcgagaact tccaccccgt ccagcaaatc ttccggcatg ggtcagcgtt tttctggccg
    54241 ctttaattgt tttcgaagag atgccacaaa gcgatcgttc tcttcgggta atcccatgga
    54301 aacgcgcagg gcccccgcca tgcccaggtc gctccccgga cggacaatga tgccgtcatg
    54361 gagaaggtcg tccgccactc gatcggcgtc ggccagaaaa accaggagga agttcgtgtg
    54421 agtggggatc accttcaccc ccaacgtggt gagctcggtg gccatccgct ctcgctcggc
    54481 gatggtcttg gtccgggtct cttccacgaa aatctcttct tccagggccg ccaccgccgc
    54541 ctcttgcccc acacggttaa cgtcaaagac tcgccgcacc ttggcgtagg actcccccac
    54601 aagtttaggg actatggcgt accccacccg aagggatgcc aagccataaa tctttgaaaa
    54661 cgtgcggaga atagccacgt tccccaactg acgaaagagc tctacccccg agacgtaccg
    54721 ggggtccacc acgtattcag cgtaggcctc gtccaccacc agaagggtct cccgcccctc
    54781 cagcgccccc gccagggcaa gtagttcgtt gcgagaaatg agggcccccg tggggttatt
    54841 ggggttgcag agcaccacca ctttggactt gggcgtacag gcgctgagca actggtccgc
    54901 ccccggagcc agggcatctc ccaggggcac ctcgacgcaa cgcgccccgg cggcacaggc
    54961 cgcgaggcgg tagagcgggt acgtgggcgt gcagtagacc acctcgtcac cctcgtccag
    55021 ggtagcttga aagagggaaa agagcacccc ttccgtcccc gcccccacca ccacctccga
    55081 gggggatacg ccccactttt tggccagagc ccggcgcagg ttgtaggccc ccgggtcggg
    55141 gtaacgcctc acgtcctgcg cggcggtcga gatgacctgg gacaaccgtt ctggcagggg
    55201 ccaggggttt tcgttggagc tgagccggat tacctgcttc aaccccagtt ctcgctgcac
    55261 gtcgctaatc ggctttccgg gccggtaggg ttcgattccc atcagggcct tgcgacccaa
    55321 acgctcaagc catgacatct tccgcgctcc tcctttcaac tgcgagaccg tacccgtcct
    55381 ttttcgtcgg ccccgccctg cagggcgacg gagcccaccg aaggctcttc gccgaggcgg
    55441 gtcctcttcc gatccctccc acgattcccc gatcaaaggg cggtgaggga gcttttttgc
    55501 agggaccgat cgccataaaa cgcaaaaaaa tcaggccaag tcttcctctt ccgaggagtc
    55561 caggtttatg gcagccccca gggaacggag cttcgtcacg atgttttcgt acccacgatt
    55621 gatatgttgg atctgaaaga ccgccgtctc ctcggacgcc gccaggcccg ccaagatgag
    55681 cgccgcccct gcacgcaggt ccgtcgcccg aacatccgcc cctcgaaggt ggggaactcc
    55741 ggtgatcacg gcggtccccc cctggacgtc gatgcgagcc cccatttttt ggagctcgtt
    55801 cacgtggagg aagcgcgact caaagactcc gtcctgtacg atgctcgtgc cgtcggccaa
    55861 agccaacacc gccatcatct ggggctgtaa gtccgtggga aatcccgggt aggggagggc
    55921 tttcagcgat agggaagata gcctggcgct ccgccgggct ctggcatcac cccccgaggc
    55981 ccaggcctcc gcccccgcct cttccagctt ggcaaaaaag gcctccagat gctccggggc
    56041 aacgtcttcc acgacaactt ctcctccggt gataatgccc gccagcagat aggtacacgc
    56101 ctcgatgcga tccgcaatgg tccgcacggt ggcgccgtgc agggcggtgt ctccttttac
    56161 gtggatcgtt cccgtccctt ccccggagat gatggccccc atggagcgca aggcctgggc
    56221 caggttggta atttccggtt ctcgggcggc attttcaatc accgtttccc cccgggcaca
    56281 gaccgccgcc atcatcagat tttccgtggc ccccaccgaa gggaagtcca tatagaggcg
    56341 acacccccgt aaccctttgg cccgcgcctg gacagtgccg tgcaccagct ctatttccgc
    56401 ccccatcttg gccagaccct tgaggtgaaa gtctatgggg cgccctccga tggagcaccc
    56461 ccccggcaag ggcaaggatg cccggccgca ccgggcaagc aggggaccca gcaccaacga
    56521 agaggctcgc atgcgacgca ccagttgggg gggggtctcc caggcgattt ccgctggagt
    56581 gtgaactcgc accgccccgt tttcgaactg cgcctccgcc cccagatgct gtaagatctg
    56641 caccatggta cgcgtgtcct caagatccgg caccctttca agctgaaggg tcccctcgga
    56701 aagcaacagc gccgatgcca ggatgggaag ggccgcattc tttgctccct gggcccgcac
    56761 cgatcctcga agggctttcc ccccctgaat cactattttc tgcccttctt tcaccgtgaa
    56821 cctcctgccg tgaggccgcc catgggcgac aagagcgcct cacaaatttt ttctgctgcg
    56881 tcccccttcc cgaaggggtt catccccttt cgagccaagg ctccgtaggc ctctgggtcg
    56941 gtcaacaaac ggtacgccgt ggagaagatg cgttcctcgt cggtgcccac cagcaccgcc
    57001 gtcccctccg tcaaggcctc ggggcgttcc gtcacgctcc gcaagaccaa caccggcttc
    57061 tccaaaaccg gagcctcttc ttggacgcct ccgctgtcgc tcaagatcag ggtgctacgc
    57121 cccatggccc agatgaagtc cggatagggc aaggggtcac agagccgcac tcgggggtgg
    57181 tgccccagga aattttgaaa cgtttcccgc accgccgggt ttcgatgcat ggggaccacg
    57241 ccccatacgt ccgggacccg atccaggata cgccccatgg cccgacagat gctctccagc
    57301 cccgcccccc aagactcccg gcggtgagcc gtgagcaaca gaacccgggc ctccggcggt
    57361 agatccagga gcgccggggt ggaagggcgg cccgccctac tcatcgtcca caggagggcg
    57421 tcgatcaccg tgttccccgt taccaccact cggtcggggc ggcatccctc acgccttagg
    57481 ttctctgcgg cccccatcgt gggggcaaac caccaggagg ccagccgatc cgtcaccacc
    57541 cggttcatct cctcggggaa ggggtgtgcc atgtctccgc tcctcaaccc cgcctccacg
    57601 tgccccacgg gaattttgcg gtaaaaggcg gccagggccg ccgccaacgt ggtggaggtg
    57661 tccccgtgca ccaagaccaa ggcgggacgc tcccgatcga agacttgacc ggaaaggccc
    57721 agcacggcac tcgtcacatg atccaaggtc tggcgctcgc gcataatccc caggttctca
    57781 tccggcacaa ggccgaagta cgaaaggggt tcgaggagca tttccgcgtg ctgccccgtg
    57841 gccagaagat aggggcgcac ctccgatcgt ttcttcaagg cgcgaaagac cggcgccatc
    57901 ttgatcgctt ccggccgggt ccccaccaca agaatcacgg tgcgaggctc tgtactcaag
    57961 ggtctcactc ctgttttatt cgatctgtcc cacgcctcta tattcccccc ggaaaggggg
    58021 cgagcatgga aaggcgcagc gtgccgtaca aaaggtagat gcctcccccc aaaagagcca
    58081 cgtggagcgc acaaagcaac aggaccaccc ctcccatggg gacccctccg tcgagcaggc
    58141 gatagtgcaa atgcccccgg tccggcgcaa agggagattt cccctttagg gagcgtctca
    58201 ggatggcgta ggccgtgtcc agtataggga cacctcccaa aaggaacaag accgccatga
    58261 gagataaaag ggggcgctgg gccataaaat ccccccggaa ctggacccac agggcccccc
    58321 acaggaatcc cagcaggtta ctccctccat cgccgaggaa ggtacgcgcc cgaggaaagt
    58381 tccaccacaa cacccccgcg cagaggcccc agagggccgc ccacatgccc cgccccccta
    58441 agatcagcgg aacggcgaaa caacaccccg ccagggaaag gcataggccg ttaagcccat
    58501 cgatgttgtt aaaggcgctg gtacatcccg caatccaaag gagaaatagg gcccgcaact
    58561 cccagggccc atccaaaggt agcaccgcaa gccctgcggc gatcaggtga aacacgaggc
    58621 gcaacgccgg ggagagggga cgcatgtcgt ccacgtagcc caccagaaag acgatggtgg
    58681 cccccgtgag gagaagaggc aaatcccggt ctccggtctg ggcgaacagg gaccaaagga
    58741 cgcacccgga ccagagcgcc agcccccctc cccggggcat gacctgcgaa tggcgcttac
    58801 gccccccggg atggtccacc acccggtagt gcaccgccaa tctcatggtg aggggcgtga
    58861 tacaaagccc ccacagcagg ccggacagag ccgcaaagac caccctccac atgtggtgat
    58921 cctacttggt gccgaacaac cgatccccgg cgtcccccaa cccggggaca atgtagccgt
    58981 ggtcgttcag gtggctgtcc aacgccgcag tgaagatgtc cacgtcgggg tggtcttgat
    59041 ggaccttttg caccccctcg ggggcggcaa tgaggcacac tagggaaatt tttctggcct
    59101 tccaccgttt ggtgagggca atggcggccg aggccgatcc ccccgtggcc aacatggggt
    59161 cgaggatgaa gatatcgcgg tcctccacgt cgccgggaag cttgcagtag tactccaccg
    59221 gcatgaggct ctccggatct cgataaaggc cgatatgccc caccttggcg ttgggaacca
    59281 ggcgcaaaat gccgtccacc atgccgagcc cggcccggag gacgggcacc accgccagct
    59341 tctttcccgc caacgacttc acggcggtgc gctccagggg agtctccacc tcggagtcct
    59401 ccagcggcag atccctcatg acctcgtaga ccatgagccc ggcgatttct tgcaccaact
    59461 ctctaaactc ttttaccgtg gtgcccttat ccctgataat gcccacttta tgctgaataa
    59521 gagggtgatc caccaccaca aggcgggggt tggtgacggc agaggccctg tctgcggcgt
    59581 cctcacgtcg ctgctcgtag tcctgtatct gacggaacct ccggagatgt cgattgccct
    59641 cgaaggaggt acccagccag acgtccacgg tttcaagggc gaccccctct cccaaaattc
    59701 gaccgccgag gaccagcaca ttggcgttat tatgctgccg ggataggcga gcctcgaagg
    59761 gggagtgaca gagggccgca tagacccctc ggaccttgtt ggccgcgatg gccatgccta
    59821 tgccgcttcc gcagacgagg atgccccggg gggccttacc cagggccacg gcctccgcca
    59881 gacgaaaggc tatctcggga taatccgctc cgggaacctc ttgttccacg ccgaagtcct
    59941 ccacgtcgtg tcccgcttcg accaggtgac ggcgcaccgc ctccttcagg cggaaccccg
    60001 catggtccga acccaccacg attcccatgt ctcgtccccc tctttatcca cagaaaaccc
    60061 cctcgtgggc cttgtcccgc gaagacgagc tccccggccc aagggaggcg gccttcccct
    60121 ccccttagcc aaggcttcgg ccaccacccc tatcccttaa ccccatttca cgcgcccagg
    60181 aggaacccgg ggtttacctc ccgtctagag aaggtctttt aagatatttt ccgccacatc
    60241 gtaggggtcg cgacgccgct cttccagatc catgaggagc tcgggggtgc ggatctcatc
    60301 ccaggcgtgc tccgccaccc gggcgatctc ccttcgcaag atgtcttcca cttctttggc
    60361 caggcgctgc caacggcgcc gacgtcccgc ctcgctgcgg aggaggaaat cccggtggcc
    60421 cttaagggcc ccgcccatgg cccccacccc ttcccccgtt tccgccaccg tctttagaac
    60481 cggcggacgc cagggacgct ctcccagaag atccagcata atattcactt cgttgacgat
    60541 gcgatcggct ccatcgcgat cggccttgtt gaccacgaag atgtcggcta tttccatgat
    60601 acccgccttc atgatctgga tgtcgtcccc catgccgggg accagcagaa ggcataccgt
    60661 atcggcaatc ttcatgatgt ctatctcgga ttgtcccacc cccaccgtct cgatgagcac
    60721 cacgtccttg ccgcaggcat cgaggatcag ggcggcctcg taggtggcct tacccagccc
    60781 ccccaaagac ccccggcttc ccatgctccg gatgaagacc gaaggatcca gggcgtggcg
    60841 ctgcatgcgg agccggtcgc ccaggatggc cccccccgtg aagggactag aagggtccac
    60901 cgccaccact cccacggaag ccccttgctc cttgaggctc atgatcagct tgtccgagaa
    60961 ggtgcttttc cccgccccag ggctcccggt gatgcccacg atgtgggccc gccccgtgtg
    61021 gggatagatg gcgcgcatga tgccgtcggc cgcagaggac tcgctttcca ccagcgtgat
    61081 aagccgagcc atggcccgca aatcgcccgc cagggctcgc tccaccgttc cgctcatatc
    61141 cgcttcgctc cttttttcaa ggggccaagg gctgggcccc catgtcaaat cccacgggcc
    61201 agtagggaat ccagtcccgc aaggtcaggc tccccgcatc cacgtagatc gaaagcatcg
    61261 cctcgctggg gtaccccgaa ggcagggcat ccaccggcag gggaacccgg atctcccacg
    61321 aagccctggg ggcagagggg agggcgtcca ggcgctccgt gggcatcacc cgtcctccgg
    61381 tcattcgcac cagacgggcc aagggcgccg agggaatctg ggtcgccagg gcgtaaaccc
    61441 ttatgccatt gttgagggcc atgctgaaga gccgctccag atcctccggc gagaagggaa
    61501 tggccgcatc caccaccaga caggaaggga gagttcccgt gtccttgcac gcctgacgca
    61561 gagccgagag gagcatcgac ccgtcttgaa gcacggcgac ggacagatct ttcgtctctt
    61621 ccgcccttcc cgctcctccc agcaggagca ctccccggtc ctcaccggag gctcggggga
    61681 cgatggtccc ctccatcttg cgtcccatcc acaccgccga aacgaagctc ctcccccggg
    61741 gaaagagtgg gcgagcaggc tctcccacgt aggcccgaag cacggcctgg gggttttctc
    61801 cccccacgct ccccggggcc gcaaggcaca tcatggtagc catccccgcc acggggggat
    61861 actccacgcc gctccagagg gcgtttcccg ccacgtccca gattattacc ccctggggag
    61921 attccgaaag ggcccaggcg tccttcagtc cctcaaggag cagcgatggt tctccttcgg
    61981 tccagagata gagcttgcct tcctcggaca agatcatcag ggcattgctt cggctccaca
    62041 gcacgtccct gggggaaggc accgaaagct ctttctccag ctggcgcttc tgggtatcca
    62101 agacgtatac tcgatttccc ccccggtccg ccaccgccat cagggggccc aaagacgaca
    62161 ccgccacggg ctcgaagggg tgatgaggat ccgctctctg gggatcccag ctccagtcct
    62221 ctcgaaacga aggtccgtcg aaaaagacca cccgccgtcg cccccagtcc gccaccccca
    62281 gcaggccgct cgccacgcac tccccgtcgg cggggatcgc cgaaagggtt ccccggggct
    62341 ccaggtccga agaatccagg ctcccttccc acacccctcc ggaacgggaa ataaccaaaa
    62401 acttttttaa atccatcggg cacaccgcca ccggggcatc ggtggaaatc ctcctcatgg
    62461 agggagacgc cccggggaaa aattgcgtca gggagtttcc cagctcgtcc gccaccactt
    62521 gggccggtcc caccatgcgc gccttggcga tcccccgggc gttccagggc atcgtcaggg
    62581 gcaaacgaaa aaaaacctcc accggagacc tctggaactg aaaactcggc gctccgatag
    62641 gaccccgaag aaggtctcgg acggagtccc tctcctcccg cagggaggcg ccgatcctcc
    62701 gggggatctc ctctcgcggg cgcacctcaa ggtagtgctc caccgccgcc gtggcttcgg
    62761 ccaaaaaacc caggcggcgt aacaccaaac cccgaaggag gtagtagtcc accaagtagg
    62821 tgtttctcgc caacgcctgc tccagggcat ccagggactc ccagtacctc tggcgcacca
    62881 ggaggtcgta agcgaggaac aaatatcgtt ccgcctcccc tcgttgggcc tccgggagcc
    62941 cctctcccca cgagacccct ccgagaacta gtaacatcag tcccgcgatc ccccctcgca
    63001 ggcacgaaga tctcatgaac gccacagcga aacccccgcc aacgagtaaa aacccaggac
    63061 gagggctccc gtgaaaaaaa agcccaccag tccccggaga taagccaccc agggggcccc
    63121 gccgggtagc gcgcctcggt gaaccaacgc caccaatcgg gccagcccca gggctactag
    63181 gaacgaaaaa agcgccgcca acgcctgaat gtttatgtcc gacacggggc gaccctcctt
    63241 ccgtcatcgg gggctcaact ttcttccccg ggcccctcga aggggcaaag cgccctcctc
    63301 gtaagcatac ccgaaattgg cacaaaaaaa aggggagaac tctcccctca ggaaaaaata
    63361 tgcttggaag gccttcatca tccgatctgt ctcagccggg ccccctcatt tccttcggcg
    63421 atctgcctaa gcaccgccac ggccacctcc aacaccctct taccgtcgcc gatgcccacc
    63481 aggggctggc gaccgtcctg tacgcaggag acaaagtgct gtaactccag ctttaggggc
    63541 tctctcttgg ggaagatggg ctgctccacc acttccatga ggctggtgcc gttgtctcgc
    63601 acgcagcggt ggatggacac gtcctgggtc tcgtagttca cgttgatgta ccgggcgggc
    63661 tccatgatct ccagctggcg caaccgccgc tcggaaaccc ggctgaccag gatgtccgcc
    63721 atggcgccgt tggcaaactg aatctgggcc gaagcgatgt cttcgtggtc cgaccgcacc
    63781 gaacgcccca tggcggcgat atggacgatc tccgaatgca ccagggacaa gatgatgtcg
    63841 atgtcgtgca ccatgagatc cagcaccacc cccacatcgc tgatgcgagg ggaaaagggt
    63901 cccagacgcc gagactggag caccaatggt tcattaacaa tatttgaaac gtactgggtg
    63961 gcgctgttga acctctcgat gtgccccacc tgaagaagga ggtttttctg ggccgccagc
    64021 cgtagcaggt cctccgcctc gtccacccgg gaggtcacgg gcttttccac caacacgtga
    64081 actccccgca ggagaaggga tcgcgtcacc tcgtaatgcg cgcaggtggg aaccaccacg
    64141 ctcaccgcat cgggcttctc tcgatcgaga aaggtgtcca gatctccgta aaaaggcacc
    64201 cgaagggact cccccgtgcc catggcgcgg tcttcgttga tgtccaccac gcccaccaga
    64261 tgggccccca agatctccgt gtaaacccga gcgtggtgat atcccaaatg ccccacaccg
    64321 atgactccga cccttatcat gacgatcaca ctccgtctcc gattggggac ttctaccggt
    64381 tcctacagaa tgttatccca ccaaaatccc ttttgtggac caggggccac gagaaaattg
    64441 cgagcctcga ggtctttccg acgataggag tagaaataat ccgaggcaca ggccgtacac
    64501 cagggggacc ggcacaagga ctcccggggc acccccaggt cgtcgcattg ctgacaaagg
    64561 atacggccga tgtccaccgt cacaagttcc tccgataggg gagcaaaggc ctcggggaag
    64621 agggcgtacc cccgcttcac ccagggatct tcccgggacc gggggtagca gcaggggcca
    64681 attcccggcc ccatccagac gcaaaggccc tcccgggccc gggggccccg gcgatccagg
    64741 aggcgccgga ggttctgggc caacaccccc gccacggccc ctcgaaaccc cgcatggagc
    64801 aacatcaccc aaggagtcgg aaaggtcccg gccaaaatca cgggccagca gtcggcgaaa
    64861 cgcaggctcc cccacacccc cgcgacgtcg agctccacgc catcggcctg ggggcgctgg
    64921 gggagggccc agatccttcg gccagagacc accgccacgc catggacctg ccgaggagcc
    64981 accaggggat ctcggcgcag gtcgtacccc agggccctcc agtgcgcctc gggatccccc
    65041 ttcgctcttt ctgcggcgtc ggagcgcccc atgaggatcg ccacggggtc atcgacgccc
    65101 ttcaggtggt agcgcacctc cgccggggag gccccggaga tccggggagg ccagagcgtc
    65161 gcctcgggaa ggagcctcat ggctcacctc ccccttcggt caacaaaggg cgcaagattc
    65221 cgatcagata aaggctccca cagaccacca cgagacgtcc ctttgcccgc gcccgggcca
    65281 gggcctcttc cggagcctcc accgatgaca cgtcgcgcca ccccgcgtgc cgggcttggc
    65341 ataccagatc gtcgggggcg gcgctcctgg catttcccgt cacccgggtg cagatcagct
    65401 ccgatcccac ggcgaggagc tggctcaggg ccccttgata gtccttgtct ttcatggaag
    65461 cgaagacgat ggtgcgggga gcgcggggcc cccagaggcg ccccagggtc ttcgtcaggg
    65521 cggcgacgcc gtgggggttg tgggccccgt ccagcaagac cggcggatct ccggggaaaa
    65581 gctctcccct tccgggccat cgggcaaggg ataatccctt gcggagggcc ccttcggtca
    65641 cggggagaag gtctccgatg aggtccaacc ccgccacggc caaggcggcg ttgcggagct
    65701 gatggggccc caggagggat atttcaagcc cttcccaccc cctggacctc ccgtaaaggg
    65761 aaaaacgccc tccggcggga gaaagggctt cctcccggag gcgccactcc gaggagagca
    65821 cccgcccttc gatccctccc cggagagcgc gctcccgaaa ggccccttcc aattcggggg
    65881 gctctcccgc gaagatggcc cttcctccgg ggcgaatcac ggcaaacttc tccgccgcga
    65941 tggcctccag cgtagacccc aaaaactcct catggtcctt ggcgatgggg gtcaccacgg
    66001 aaaggagcac ccgggacgcc atgttggtgg cgtccaagcg tccccccagt cccgcctcca
    66061 gcacgaccac atcgggagcc tgggtgcgga ggaggcaaaa ggtcacggcg gtcaccaact
    66121 cgaaaaaagt cggaggatta gcccggagct cccggtctcg gtccagggcc ccccagatct
    66181 cctccgccgc cccttcccac tcctggggcg agaggggatt tccctccagg gtcagtcgtt
    66241 gcccggggga ttccaggtgg gggctggtgt agagcgccgt gcggtacccc tgggcccgga
    66301 ggatggagtc caaaaaggca caggtcgacc ccttgccgtt ggtgcccacc acgtgcaggg
    66361 cgggcaccgc gcgctcgggg tgtcccaaga gccccaagag ccgagatatc ctcgccagcc
    66421 cgggacgaat tcccgggctg gccgccttcg tcagtcgatc ttccaggaaa tcccagggat
    66481 tttccgcccc catgaaggcc tacctcaggc tctccaggtt ctccgcgatg cgcgccattc
    66541 gaagcttgcc gtctcgcatg cggtctcgct ccttgcagac cacctcttcc ggggcccggg
    66601 agagaaactt ctcgtccctt agcttggcct cgcagagttc cacgtctttg gccaatttgg
    66661 cccgctctcc ctccaggcgc cggagctccg cttcgacgtc caggagcgat cccacttcca
    66721 gataggccgc aaaatccggg gcgatggtca ccagggatcg ggcgggcttg tctcccctcg
    66781 gaagcaccac cagatggtct accttggcca gaagacacgc caggtccttg tgatcttcca
    66841 ccacctggcg cctcccctcc ccggggatca ccacttgggc cgagggcacg gtctgagagg
    66901 gggcgatgcg ggcctcggag cgcaggtttc tcagcgtgcg caccacttcc tggagcagac
    66961 ccatctccgg ccctgattct tcgtcgccgt tcacggggtc ttcgggccat ctctcctggt
    67021 ccatggggcg ggccccgaag ccgaaggctt cccacaactc ttccgtcacg aaggggataa
    67081 aggggtggag caaaagcaac gtggtgcgaa aaacctggcg gaggaccttc tgggtggccc
    67141 ggcggcgccc ttctccctct tctccccgca gggcgggctt ggccatttca agataccagt
    67201 cgcagagctc ggaccagacg aaatcgtaga gcccccgggc cgcctccgcg tagaaatacc
    67261 ccgaaaggtg ggccgtggtc tccttcgcca cctctcggct acggcgcaag atccaacgat
    67321 cggggagccg caaggggtcc tcgggatcca gggaaccttc gggagctccc tccaagttca
    67381 tcagggcaaa gcggctggcg ttccagagtt tattcatgaa aaatctatat gtctcgatgc
    67441 ggctccgccc cagcaagatg tcccgcccct gccccgtgag ggccgccagg gtaaagcgca
    67501 gggcgtcggc accgaactca tcgatgatgt ctaaggggtc caccacgttc cccttggatt
    67561 tgctcatctt ctggccccgt tcgtctcgga tgagggcgtg gatgtaaacc tcccggaaag
    67621 gaacatcgtc catgaactcc aaccccatca tgatcatgcg ggagacccag aagaagatga
    67681 tgtcgaaccc cgtgaccaaa aggctcgtgg ggtagtacgt ggccagatcc ggggttttat
    67741 cgggccaccc cagcgtggag aagggccaga gggcactgct gaaccaagtg tccagcacat
    67801 cctcgtcctg ggccaacgtc gaggcgccgc aacgctcaca ggccgagggg tcctcgctgg
    67861 ccacggtgat gtgaccgcac gcggcgcagg tccaggcggg gatgcgatgg ccccaccaga
    67921 gctgacggct gatgcaccag tctcggacgt tttccatcca ctggtagtag gtgtgggtcc
    67981 attgctcggg gacgaaacga atgcgtccct cccgcaccgc ctgaaccccc cggtccgcca
    68041 gcggcttagt ccgcacaaac cactgctccg acaagaaggg ctccaccacg ctcttgcatc
    68101 ggtagcagtg ccccacggcg tgctggtgtt cttcggcctt gagaaggagc ccttctcgcc
    68161 caagggcctc caccaccagc ttccgagcct cgaaacgatc cttgcccgcg tagattcctg
    68221 cagcttcgtt catgatgcct tcgccgtcga tgacctggat ttgctccaga ccgtggcgct
    68281 gccccaccaa aaagtcgttg gggtcgtggg caggggtgat tttcacgcac cccgtgccga
    68341 actcaggatc gaccatgttg tcctcgatga cgggaactag gcgtcccgtc agaggaaccc
    68401 gcacccgacg ccccacgaag gatcgattcc ggtcgtcccg gggatgcacc gccacggcca
    68461 cgtctcccag gatcgtctcg gggcgggtgg tggccaccgt cagggctccg tcaccctcct
    68521 cgaagggata ggccacgaag tagagctttc ccgccgtgtc gtcgtgatcc acttccaggt
    68581 ccgacagggc ggtatggcat cgggggcacc agttgatgat gtatttaccc ttgtaaatca
    68641 ggtccttctt gtacagccgg acgaagacct ctcgcaccgc ccgagagagc ccctcgtcca
    68701 tggtaaaacg ctcccggctc cagtcacacg aggcgcccaa tttcttcagc tggctgatga
    68761 tgcggcttcc gtactgctct ttccaggccc agacgcgctc cacgaaggcc tcgcgcccca
    68821 ggtctcggcg ggatgtcccc tccgaggcca gctggcgctc caccacgttc tgggtggcga
    68881 tgcccgcgtg gtccgtgccg ggaagccaca ggacgttgtc gccgcgcatg cgatgccagc
    68941 gacacacgat gtcttgcagc gtgttatcca gcgcgtgtcc caggtggagg gatcccgtga
    69001 cgttcggggg gggaatgacg atgcaaaagg gttttttccc ttcttcgtgg ggatctgccc
    69061 ggaagaggtc ctcggccatc cacgccgcat atcggcggtc ttcaatgggt tttgggtcgt
    69121 acgccttgcc tatctcgcca cgcggatcca tagtagtttt ttcctcctcg attctcccat
    69181 cccctcaccg gggtgatggg ccacgcctcg taaattcttc gaccaccggg acgatcacca
    69241 gggctcttcc gaactcggcg ccccaaaagg ccccggaggg ctcaagcccc cggtcaggcc
    69301 ccttcacccg ccgccccacc ctccggggcc cggggacttc tggcgagcac cacgccctct
    69361 aaaaagctcc ccagctccgc cacgccctgg cgtttcgccg ccccggtcac caaagggggg
    69421 tagatcgaca ccatgcccgg gtaggcgtac cgccgacaaa gccctccatg gtgcgcccgg
    69481 gcaatcttat ccgctttggt gaagaccacc tgccccggta gcgccagatc tctcatccaa
    69541 ccctgcatca tgaggtcgtt ttcgaggaac ccgtggcgga aatccacgag ctggagcacc
    69601 aggcagaggg ggcttcgatc tcggacgtac tcctcaatga ggcgccccca gtggcgccgc
    69661 tcctcgtgac cccttttcgc gtaaccgtac cctggaaggt ccaccaagta gaaggacaga
    69721 tcggtgcggg ccaccctgat gcgaaaaaag ttgatgctcc tggtcttccc cggggccgag
    69781 ctcacgtgcg ccagcttggt gcctaaaagg gcgttgatca gcgtggattt tcccacgttg
    69841 gatcgccccg ccacggcaat ttccggcagc gatggcggcg gtatctggtc cggggtaaag
    69901 caggtatgcc ccagagaaac gtgccagtgc cccatggcta ggcgctctgc ccgagggcca
    69961 cggccaccac ctcggacaga tggtgcacgt agcgcaaggt gaggcccttt cgtatccagg
    70021 ccggaagctg ctccacgtcc gccaggttgg cggcgggaag gatgatgtcg aaaatcccct
    70081 gtcgtttcgc cgccaggacc ttttcccgga tgccccccac ggggatcacg ttcccccgga
    70141 gggtgatctc ccccgtcatg gccacgtcat ggcgcacctt ccggcgcccc agggcggaca
    70201 acatggccac cgccaaggtg atccccgccg aaggaccgtc cttggggatg gccccctccg
    70261 gtacatggac atggacatcg gtcttgtccc aggagacctt ttccagccca tattgggaag
    70321 ccccggagcg caggtacccc agggccgtct gggccgattc ctgcatgatg cttcccagat
    70381 ttcccgtaaa agtcaccgcg cccttgccct tcatgatcac cgcctcgatg atcaagacgt
    70441 cgccccccgt ctccgtccag gcgagcccca ccgccgcccc ttccgagtcc tcgtcgggga
    70501 ggcgcgtgtc ccgcgccagg ggagctccta aaaactgccg gagaaccccc ttcgtcacct
    70561 gaaggggaga ggagattttt tcttttttct ccttggcttc caccaaaagg cgagtggcct
    70621 tacgggccac cttggcgagc tgacgctcca ggtggcgcac ccccgcctcc cgggtgtaat
    70681 ccgagaccac cgtctggatg atccgatccg ggaggcgcac gtcggattcc ttcaggccgt
    70741 gctcttccag gaccttgggc cagaggtgtc tccgggcgat gtggtgcttt tcttcggcca
    70801 cgtagcccgg aatctggatc acttccatgc ggtccaacag ggggcgcggg atggtgtgcg
    70861 ccacgttggc ggtggtgagg aataagactt tgctcaggtc gaaggggacc tccagaaagt
    70921 ggtcggagaa ggtggcattt tgttccggat ccaaaacctc caaaagggcc gccgccggat
    70981 cgccccggaa atcggccccg atcttgtcga tttcgtccaa aagcaacacg gggtttttgg
    71041 tgccgcaaga tcggagtttc tggagaatcc gtcccggcag ggctccgata taggtccggc
    71101 gatgcccccg gatctccgcc tcgtcccgaa ttccccccag ggatatgtta acgaattttc
    71161 gattcatcgc ccgggccacc gagcgcccca gggaggtctt tcccaccccc ggggggccca
    71221 cgaagcagag cacctgcccc ttgatccgag accgggcgcg ctttcgcacc gccaaaaact
    71281 ccaggatgcg ttctttaact tcctccaggc cgtagtggtc ttcttccagc acctgtttgg
    71341 ccacgccgat gtccagccga tcccgggatt catgggacca gggcatggcc gccatccagt
    71401 cgatataggt tcgcaccacc gtggcctcgg cggacatggg gggcatcttc gcgaagcggt
    71461 ccagctcctt caaggcgcgc ttttccacat cggggggcat ctgagccccc aggatcgtct
    71521 gccggtactc ctcgacctcc gacatcccct cgccgtggcc cagctcgtcc tgaatgaccc
    71581 ggagttgctc ccgcaggtaa tactcccggt ggctccgctc catttcctgg cgcacccggt
    71641 cgtggatatc gtgctccaac tccagaattt cgatctcttc cagcaatatt ttgagcaaaa
    71701 gagccagacg atcctcttcg cagaggactt ccaggacgct ctgcttttga gggaccttga
    71761 taagcaggtg ggatgccacc agatcggcga attgccccgg gtcttccacc gaaaagacgg
    71821 acatggccac ttcgttgggg atcttgggat gcaacgccac atagcgctcg aaatgctcca
    71881 tgacgccacg ccgcagggcc tcgattctct caaggtctcc agaggattcg agcaccggcg
    71941 acaaaaaggc ttcgaagtag tccttctccg tggagaacga ctctaccgcc gcccgctcca
    72001 tcccctccac cagggcctta gtggtaccat cgggcactcg gaccatctgc aacacttggc
    72061 acaaggtgcc gatgcgaaaa aggtcttctt ccctagggtc gtccgttatc atctcgcgct
    72121 gggccaagac caagaccttg cgctctttga gcatggcgtc ctccagcgcc tttagcgacc
    72181 ggggacgccc tacgaacagg gggacgatga tgccgggaaa gagcacgatg tcacgcacgg
    72241 gcaacacggg aacccggagc gtcgccctat cgcccatcag gcaacctcct tgccgtcacc
    72301 ggaaaagagc gccgggtggt ccttttcttc cacagtacga tcgttgatca cgatgcggga
    72361 aatgccctct ttggaggagg ggatggtata catgagatcc agcatcaagg actccatgat
    72421 ggtgcggagc ccccgggccc cggtgttacg cttgaacgcc tgggccgaga tggaccgcag
    72481 ggcttccggg gtaaactcca ggtcgatgcc ttccatctcg aaaaccttac gatactgcct
    72541 gacgagggcg ttgcggggct ccaccaaaat ccgcaccagc gcctcctccg aaaggggttc
    72601 cagggccgcc agcacgggca ggcgccccac gaattccgga ataaagccgt aggacaccag
    72661 gtcctccggg agagtttccc ggagaagctg gtaggtctcc gtcttgccgc tccccactat
    72721 atccccgccg aagccgatga gtttgcggtt catcctgcgg gccacgatgt cctctaaccc
    72781 cgcgaaggcg cccccgcaga cgaagaggat attggacgtg ttaatttgaa tgaactcttg
    72841 atgagggtgt ttgcggcctc ccttgggggg cacgttggcc acggtaccct ccaggattcg
    72901 cagcagggcc tgttggaccc cttcgccgga gacgtcccgg gtgatggagg gggattcgga
    72961 ctttcttcct attttatcca cttcatccag gtagatgatc cccttctcgg cggcctggat
    73021 gtcgtaatcc gccgcctgca acaggcgcac caggatgttt tccacgtcct cccccacgta
    73081 ccccgcttcg gtgagcgtgg tggcgtcggc catggcaaag ggcacgtcaa gcttgcgggc
    73141 caggctttgg gccagaaggg tcttccctga cccggtgggg ccgatgagga gcacattgct
    73201 cttttggagt tccacgtcct ggtcgagctc tccggcggag gcgatgcgct tatagtggtt
    73261 gtaaaccgcc accgagagca cctttttggc gaggtcctgg ccgatgacgt actggtccag
    73321 gaactccttc agctccacgg gcttgagcat ctcccgggtg cgggaagccc cctccatccc
    73381 cccaccttcg gcgccgtcgc cgctactcaa aattagatta caaagactga cgcactcgtt
    73441 gcagatgtac acgttagagc ccgctatgat ccgctgtact tccttctgcc ccttgccaca
    73501 aaaggagcag tggggaccac gccgcttgcc gtctttgttg tcgccttcgt acttaaacat
    73561 gcctgacctc cccaagttcc taaaaaaagc ccctctcggt ctgcggaccg tttcacccgg
    73621 gaagaccgat gatgacgcaa atctccgggg ggcccgcgag gtcacagaaa agggccgccc
    73681 ccatacccac cccattgaag actgatatta ctccaatctt caaagaaaga caaacggaga
    73741 agcccccggg ctcctccgtt tgtctcgcgc acgcccttct ccctaccgcc ggtctatcac
    73801 ccggtccacc atgccatacc gaaccgcttc ctccgccgac atgaagaagt ctcggtcggt
    73861 atcagcggcg acacgtttca taggctgtcc cgtgtgctgg accaggatcc ggttaaggat
    73921 gtctttcagg cgtaggatct cttttgcctg gatttcgatg tccaccgcct gtccctggga
    73981 tccccccgag ggctggtgga tcatgatccg ggcgtaggga agggcaaagc gcttccccgg
    74041 agcccccgcc gccaagagga ccgccgccat gctcgccgcc tgccccacac agatggtgga
    74101 cacggggcac ttgacgtact gcatggtgtc gtagatggcc atccccgagg agatgactcc
    74161 cccggggcta ttgatataaa gtgaaatgtc tttgtcggga tcttcgcttt ccagaaagag
    74221 aagctgggcc accaccaggt ttgccacgtg gtcatcaatg ccgtcgccga ggaagacgat
    74281 acggtctttc agcaagcgac tgtatatgtc gtaggagcgc tcacctctcc cggtttgctc
    74341 tatgacatag gggataagcg gcatcaggcg ttcccttccc ccgaggcatc gaagccgccg
    74401 tgcttgcccc gatactcctc cataggcatc tcccgcaccg ccaccttgga caccaagtgg
    74461 tccagggtct ttctggtgcg cagggccgcc atgagctctc ccaggaggtc cctgcgctgg
    74521 gtgtactgct cgtacacctt cttgagcggc gtaccggtgc ggagcgcgac ccttcgaaac
    74581 tccatctcca ggtcttcctg ggaaacctcg atctggttct ggtccgccaa aacatcgatc
    74641 acgaagccgt ttctggtcag cgcctcgccc cgctcccgca gcccctgatc gtaggccgcc
    74701 aagtccttgc cctgggccct caggacctct tccaggtctt ccttgccctg gcgacggagc
    74761 tcttccagat cgtcctggcg gagctggttc atgtgccgca acaccgattt ctccggcaaa
    74821 gaggccacgg aagccttcgc cacgagttgg tcgaaggcct cgtttcggac catcatatcg
    74881 acccgggact gcacctccga ctcgagacgg cggcgcatgc gacggcggag ctcttccaca
    74941 gtcttcgccg ctccagaagt gagctgaggc acggtctcgt cggtgagctc ggggaggatt
    75001 tcctcctgga tgtcggtcac cacgaagtgg taccggacgt ttctccccgc atagcgggga
    75061 ttggcgtcct cttccatgga caccatcacg tcgacctcgt ctcccttctc acggcccata
    75121 aggacgtcgc ggatctcctt ccgcaactgg ggagctccta gggcgatggg agaggtgttc
    75181 gtctccactg gatcttcttc tccttccact gtggcggtgt atgacaccat caccgtgtcg
    75241 ccctctcccg ccggacgccc ttccaccgaa gccatggtgg ccctaacccg acggaactcg
    75301 tccatggctt tctcgacgtc atcctcggcc acatcgatca gtggtttatc cacctcaatc
    75361 tcttccaggg gaggcagggt tacttccggg cgcacctcca gggtgatgtc caccacgagg
    75421 ggctttccct cttccgccga gataaagtta aaagaagggc tttcgatcgg cgccaggtca
    75481 tactcctccg cgagctcgtt caccacgcgg ggcatgagtt cttccaaagc ctcgttcacg
    75541 atggctcgag aaccgaagta catatccagg gcccgtcgtg gcacgtgccc cttgcgaaac
    75601 cccttcaact ccacgttcct tgacatctcg cgcacggcat cgcgcaccgc tccatcaaac
    75661 cgctcctgct ctacctccac atgcaggacc actgtggtat tttcctgaga cacgatttcc
    75721 gaacgcaccg tcgtagcgcc cctttcttaa attgccaagt ggtttgcctt actcacacaa
    75781 aaaaaccccg atggtgagcc catcgaggca cgctctccct tttttctgca atccgagcca
    75841 gaaaacgacg aaaggtcacg gtcctcttca tctccttttg catcctagca tggggagtct
    75901 caatcgtcaa tcgcccccca atcttggctc gtcagtgaca tgggggcgtt ttttcgccgc
    75961 ccaggtcccc cggtaaatca tggtttgggg tcttccacca ggggagtttt cccccaagcc
    76021 acccatccct ccagggtaac tagcaccacc gccgtgccta tgaaggccgc tcctcctagg
    76081 gtagcgggcg tggggatttc gccgaggagc caccaccccc aaaagatggc aaagagcatc
    76141 tccgtcatgg agagcaacga caccaccgcg ctgggaacgc gccggagcgc ccacattacc
    76201 agaccgtagg cccccagcgt ggggatcagc accaggtagg ccagcagggc ccacccttcc
    76261 caaggcaggg cccggagatg agagagggtc gacgtgaagc aggccaagcc ccacatcacc
    76321 gccgtccccg ccacgtaggt gtgaaagtag gtcccgaagg tatcgtcggc cccgggggca
    76381 tagcgtccat tcagcaaaaa gaccgcatag gaaaatcccg ccaccagcat ggccacaggc
    76441 ccccacaggc cgtacccgag aaagtggatc tcccctgcgg accaccacac tccccccagg
    76501 gccatcccca ccgacacgat gcgcaggggc gtcaggggtt ccttgccaag gacatgggcc
    76561 aacaccacca cccacagggg ggcggtataa aacaggacgg tggccaaccc caccgggatg
    76621 gtgatcaggg cataattgct caagacgtaa agtcccacca ctccgaaaag gccatttacg
    76681 gcacggtaaa ggatcaacgg gcgcgggcag cgcaacgact tccccctaag gaagcctatc
    76741 cccccgagca gcaacgccgc tcccgtggcc ctcaccagag agatgtcaaa agacgtcaat
    76801 ccgtaagagg ctccctggcg catgatgggc cccatgacgc cccatatgaa ggtacaacag
    76861 agcaccgcca gcactccccc catcgccatc tccccaattc ctcctgttat atcgtttaag
    76921 ccaaaaagtt tattatagca gacaaaagaa aaaatgtcct cccccttatg gccctttctg
    76981 caaaaaactc ccccatggta gaataatgga ttatgatcgc ctgcctctac cactttacca
    77041 aaaagccctc cggaggcaag acctttcgtt ttccaaaaag ggcgcgatcg gagaggaacc
    77101 tcctcctaag cgccggggag ggctccgggg caacggcgga acttcttgat gcgtggaaaa
    77161 attcttcgag agaggaggga atatccatcc cgcccccgac ccttgcgggg ccaaaaaaga
    77221 aaggagggcg tcatgacccc acaccctttc atccagcagg gctgtgccct ggtgaccatg
    77281 agtaccggat tgcgggccca aagcctgaag gagctgcgcc agggcgtgtc tttggcgagc
    77341 gaccagtgct tgcactacca cttctggggc cgtctactac gtccccaatt cggcgaggcg
    77401 gatttcgcca acgactttgc cgcctgggtg gcgggccctg tgggggatcc cgccacggcc
    77461 gagcgcctga atatcctcaa tccttcccat gtcccctcat tcgaagacct gcgacgagat
    77521 atcctcgaca tcctcgacga tcgtctatcc cgagaggact tcctctcctg ggcccgagcc
    77581 gatcgggatt ttttcttcac cgacgccgcc atgatcgtct tcgacacgga gctccgggcc
    77641 cactcccccg cagagctggc ctccctggtg gaaaacgcca gccaggggtc gctatttttt
    77701 cactttgttg acgcccggcg gcgcacgccg gaccaccacg acgacttttc ctcctggctc
    77761 gtccacgcct gtgaagaccc cgaagatagc cctcgtatca gggctctgag agccctggat
    77821 ccctttctcc tgagggtcac cgagctccgt cagcgcgtgg gagcagccct gagaataggc
    77881 gaagaggagg ccccggcatg aaaactctcc tggcatcgta tcgggaagtg gtgggatcgg
    77941 aagagatcgc cgtattggaa caactggctt cgcgcctcca gggccgccga gtggtgcacg
    78001 tgaactccac tcgcgagggg ggcggcgtgg ctgaaatatt gcagcgtctc atcccgcttc
    78061 agcaggaact ggggttggag gcccgatggg agaccgtgga cggccccccg gaattctttc
    78121 aggccactaa aactatccac aacacgttgc agggcgccaa ggggaacctg gccgcgaggt
    78181 accgagacgc cttcgaaggc gccgggctcc gcaacagcga aaagctaaaa aatatcctcg
    78241 aaggggcgga tttcgtcttc atccacgacc cccagcccgc tcccctcatc caacactgct
    78301 ccgatcgccg ggggtgctgg atatggcggt gccacatcga tctcagctcc cccagcagga
    78361 cggtgtggcg ttacctgcaa ccctgggtgg cctcctacga tgccagcatc ttctcgctac
    78421 cagacttcgc ccaacccctg cctcacccgg tctttctcat tcccccgtgc atcgacccct
    78481 taagcgataa aaacatcctt ctccccgagg aggaggtcct ttcggtgttc gaccgcttcg
    78541 ggctggatag ggcccttccc ttggtcctac aggtctcgcg cttcgatcac ttcaaagacc
    78601 ccctgggagt gatcgaggcc tgccgcattg tcatgaagac cgcccccatg caactcgtcc
    78661 tcgccggagg aggggcctcg gacgatccgg agggagaggc ggtcctccaa gaggtgcgcc
    78721 gcgccgccga aggcataccc cacgtccaca tcctggtcct tccctcggac gcccatcgca
    78781 ccatcaacgc cttgcagcga gccgccgccg tgatagtgca aaaatccata aaagagggct
    78841 tcggcctcac ggtaaccgag gccatgtgga agggcaagcc cgtcatcgga gggaacgtgg
    78901 ggggcatccg gctccagatc atccaggggc aaacgggcta tcgggttcac accccggagg
    78961 ggctcgcctt ccgcttacgc cacctcctac agcgccccga ggagggccag gtcatgggga
    79021 aaaaagggag ggagtacgcc ctggaaaact tccttttgcc ccgcctcgtg aggaacgagc
    79081 tggctatcat gctgctccta ctccaggaaa ccccccatcg actggaggtc accgtctaga
    79141 tgatctccgc cgcgctctcc gtcgccgggg ccctccgggc gagcctactt cgggcttccc
    79201 ggagggtact gggactggat ttcgacggaa ccttggcccc ttttcgccct cgccgcatgg
    79261 aggtaacccc ctaccccggc gtcgtcccca tgctggaaaa aatccacgcc cagggggacc
    79321 ttttggcctt tttctccggc cgccccgtgg cggaggtgcg ccatcttctg aagcccttgg
    79381 gagatcggtg ccccatattc ggatgccacg gcggagagga ggggtggccc ggagaggagc
    79441 cctttttgct ccccctgcca gaacaaaccc aggaggggtt ggccgttgcc gaagccatgt
    79501 ttcgggaacg gtggaagcat aaagggttca cccttccctg tcccctggag cgcaagcatg
    79561 ggaccttggc gcttcacacg aggaattatc gactgccaga acaggatttt tccgtggaga
    79621 tctgggcctc ggtggctcgg gagacgggat tacagatcct gcccttcgac gggggatggg
    79681 agctgcgtca cagagccttc aataagggca aggccctgga gcgatacctc gcctccttgc
    79741 ccccccccga gcccccttcc gacttcgtgg cctatctggg ggatgatctc accgacgaag
    79801 acgccttcgc cttcctggcc cgccggggtc tgggaatcct agtgcgatcc cggtggcgtc
    79861 ccacccaggc ctcggcgtgg tgcgtgcccc cggaagaggt ccttcaattt ctttctctgt
    79921 gggagaaata attcaaaaaa ggagaggtct aatgacagaa aatcgttatg aacgcctagt
    79981 ggtggtatcg aaccgtctcc ccgtggtgct ccatcgtcag gaggactcgt ggcagatcaa
    80041 gggaggatcg gggggcctcg tcagcgccct cggccccgta cttcgagacc gaggaggggt
    80101 ctggatcggc tggcccggca cctctgagac catcggaatc cgggaagtac gaaacatcct
    80161 aggccccgcc tccgagagca gcggctatcg tctctttccc gtttttctct cgccggaaga
    80221 ggtggaaagc ttttaccagg gcttttccaa tggcatcatc tggcccctct ttcacgatct
    80281 ccagggaaag tgcgagttcc tcccggacca ttggcaggcc tacctccgag tcaac
//
LOCUS       DAOOER010000002        75399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_338_length_75399_cov_9.401372, whole genome shotgun sequence.
ACCESSION   DAOOER010000002 DAOOER010000000
VERSION     DAOOER010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 75399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 75399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75399
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_338_length_75399_cov_9.401372"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="PL162_00380"
     CDS             complement(<1..713)
                     /locus_tag="PL162_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90516.1"
                     /translation="MTRQTDQIGFSQRVRLEWFEQTVNLILAGNDKAAVMNTLQDLLQ
                     DKVSVGGQAERGNREKIITILLRTWLTVPKGLESWRDEGLELLSCAPRCDHVAFHWGM
                     IMAVYPFWGEVAIQTGRLLRLQGSAAAAHVQRRIREQYGERDTVSRAARRVLRSYVDW
                     GVLKESGVKGVYAAGTPLAVEDPRPIAWLVEASLYARGTALAPLKGLLDSPGLFPFRI
                     KAVPADGLGAVSSRLDFLR"
     gene            complement(710..2743)
                     /gene="pglZ"
                     /locus_tag="PL162_00385"
     CDS             complement(710..2743)
                     /gene="pglZ"
                     /locus_tag="PL162_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-3 system phosphatase PglZ"
                     /protein_id="HQF90517.1"
                     /translation="MRSWRDQILKEFTPKVSRLTLAADPDGLLLEEGMLEGIRGRGFE
                     LMPFEDHIAFRYAYESKFRSRWDQGEDTDLVVVLRSQASDPGGLPHDLLRAGRKLSFH
                     LGDIFPSLSYSVVAALDRGDLDALYGALVPHPPAPLGDNATRDFILRHVFDIAPELVR
                     HPTDLLHTLLRRHYRRQNFGPDLDGRFIQLLRQNAAFNDWPLEILVSDREAFFAFLQE
                     RWPRFLDHEAAKGLSRVFAPRALSPLAVAGPAELSFDHEDIRVYIDNLFVEGILHAVP
                     HDHGDVLSRTWVGIGVRTDPTEDRLRRLDKLLDRLEGSIPAEEAKHPDWFHFARGWAE
                     TILLANPPDGPVPAPGGERFRSLQARVDGAFAAWLVKRYAGLVNLPPEPPVMLHHLPR
                     FLARQMGEDRKAKIALIVVDGLALDQWLVVREALASRHPGLRFREEAVFAWIPSVTSV
                     SRQATFAGKIPLFFSKSIQTTDREPALWAQFWRDQGLTPQEVVYRKVLKDEDLEKVAD
                     DLSHPKAKVVGLAVDKVDKIMHGMALGTSGMHNQVGQWARQPYVGALLDLLLERGFLV
                     YLTSDHGNIEAEGCGRPSEGAVADLRGERVRIYPDGALREGVHKRFPDAMAWDPVGLP
                     EKYFPLLAPPRRAFVREGERIVSHGGISVEELIVPFIRIDMPGGSAQAERRVP"
     gene            complement(2803..5715)
                     /locus_tag="PL162_00390"
     CDS             complement(2803..5715)
                     /locus_tag="PL162_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HQF90518.1"
                     /translation="MKKDQNNQANLFETSPSTGDLGSVTCLGKTFPDDNARREHFLGL
                     LREKLQDPEFRKIEGFPLGEDEAILALSDPPYYTACPNPWLGDFLAAEETPETPPAPP
                     GDENTSPSENPSAGGTVAYRREPFAVDVSEGKTDPLYKAHSYHTKVPHRAIVPSILHY
                     TEPGDVVLDGFCGSGMTGVAAQMCGDKGVVESLGYRVDRDGTISRQETDENGKPVWRP
                     FSKIGARRAVLNDLSPAATFIAANYNMPFDVEAFASAGKELLDAVEQDLGWMYETWHS
                     DGKTKGRIEYTVWSEVFRCPECSGEVNFLDEALDEDSQRVRDTFPCPHCGAEMSKKKL
                     DRIRISKLDPATGKILQAPKRVPSLISYRVGKIRHEKKPDATDLTLLEKIEVLPLPAA
                     VPLVEFPFADMWEAPRMRDKGITHTHHMFLPRAAQAMGALWARARAHPEPRLRAFLLF
                     MVEQAIWGLSVLNRYQPIQQGRPGGSQVNRQLTGVYYVASQHAECSPWYILEGKRDRL
                     AKAFQHFKTGAGGAAIGTGTAARLLLPENSIDYIFTDPPFGENLPYAELNFLVEAWLG
                     VMTQAKLDAIVDRAKENRAAQKSVDDYRKLMAGCFEEYFRVLKPGRWITVAFSNTRAA
                     VWNGLQTAMRQAGFVVANVSVLDKQQGSFKAVTTAVAVKQDLVISAYKPNGGLEERFH
                     LEADTEEGVWDFVRTHLSYLPVVKKHGAVLQFVPERDSRILFDQMVAYYVRKGYPVPL
                     DSAEFQEGLAHRFPHRDGMYFLPEQATEYDKKRITTDGMEQMSLFVSDESSAIAWLRQ
                     LLKDKPQTFQDIHPLFIKELGGWQKYEKTLELSELLEQNFLRYDGKGAVPNQIHSYLS
                     SNFKDLRNLPKDDEALRAKGKDRWYPPDPNKAGDLEKLRERSLLKEFEEYKASGTRKL
                     KVFRLEAIRAGFKKAWQERDYATIIAVARKVPENVLQEDSKLLMWYDQALTRSGGE"
     gene            complement(5794..6048)
                     /locus_tag="PL162_00395"
     CDS             complement(5794..6048)
                     /locus_tag="PL162_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90519.1"
                     /translation="MTLLPLADEELDGALGLVTTVDGTRRPTVVGLLLLGHEETIRQH
                     IPSHEVAFQILEGTDVRVNEFFRKPLLQTFEEVELLTRES"
     gene            complement(6128..9925)
                     /locus_tag="PL162_00400"
     CDS             complement(6128..9925)
                     /locus_tag="PL162_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6079 family protein"
                     /protein_id="HQF90520.1"
                     /translation="MTYGDLIQFEPIESVVALRNADKAAEAQQLVQTYVISSEMADRW
                     TDIIVPQLQFDKPLDHKGLLVVGNYGTGKSHLLSVISALAERGDLSPHLNDTSVAEAA
                     GKIGGRFKVVRTEIGATIMSLRDILVAELEEHLAAMGVSYSFPPADKVSNNKRSFEEM
                     MTAFHQKYPDHGLLLVVDELLDYLRTRKDQEIILDLNFLREIGEVCKDLRFRFIAGVQ
                     EAIFDSSRFSFVSESLRRVKDRFDQILIARRDVKFVVAERLLKKTAHQQEKIREYLTP
                     FAKFYGRMNERLDEFVRLFPVHPDYIDTFERVTAVEKREVLKTLSLAMKKLLSQNVPQ
                     DHPGVIAYDGYWATLCENSSFRSVPDVRAVMDCSAVLESRIQQAFTRPPYKPMALRIV
                     HALSVHRLTTGDISTPLGATAEELRDGLCLYQPEIEDLGGTPADDLLSQVEIVLREIH
                     KTVSGQFISSNRDNRQHYLDLKKTDDFDALIEKRAESLDASQLDRAYYEALQQVLECA
                     DQTYVTGYKIWPHELEWQEHKAMRQGYLFFGAPNERSTAVPSRDFYLYFIQPFDPPHF
                     KDEQKGDEVFFRLRLESPKKPQPSGGSPRLFDPGQPPDPAETFRTALRSYAAALDLAS
                     TSSGHANAVYKDKRGKALQDLAEWLRQHMTKAFDITHQGRTKSLTEWAKGKPFRELSG
                     IGPQEHINFRDLVNTVAGICLGPAFHDQAPAYPKFSVLITGANRAQAAQDALRAMAGQ
                     NRTKQATAVLDALELLDGNRLDPGQSPYAKYILDVLGKKGSGQVVNRSELIHDILGVE
                     YLAPGGFRLEPAWAVVVLAALVYAGEVVLSIPGQDFDATGMALLAGTAVDELVNFKHI
                     KHPKDWNLPALKNLFELLGLASGMAQLVTQGKDEPVQELQKAVTNAIEEVLRAQRRLE
                     SDVFFWNRSLLNDEEMKKLSETLRKTKEFLESLKPYSTTGKLKNFRYGAEEVAAQRSG
                     LHTLAEVKSLEDLVGDLRPAASYLSIAETILPTDHEWITTMKTARHEVLAQIVDPAKR
                     SAPNFRTETQRKLDDLKRAYVRAYLSLHAKARLGVNEDQRKAQLLGDGRLKNLQDLAT
                     IDLMPRQQLLDFRNRLAGLRSCSALTERELEASPVCPHCEFKPGAEPPTAPAATMLDA
                     LDGELENLVENWTQTLLANLEDPTTKDNLHLLKPEPHERVDGFIKTRTLPEPLDQGFI
                     SALVEALSGLTKVAVKADDLRNALLAGGSPATPGELRKRFEEYLDGLTRGKELGKVRI
                     VLE"
     gene            complement(9960..10562)
                     /gene="brxF"
                     /locus_tag="PL162_00405"
     CDS             complement(9960..10562)
                     /gene="brxF"
                     /locus_tag="PL162_00405"
                     /inference="COORDINATES: protein motif:HMM:NF033453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX-3 system P-loop-containing protein BrxF"
                     /protein_id="HQF90521.1"
                     /translation="MGEPLADKIMEKINQAAELYHRLVLVVAPAGAGKTAALREVQRR
                     TGAPLINVNLELSRALLDLTERQRALQASRLLAEIVDASGVPVVLLDNTEILFDPSLR
                     QDPLRLLQGLSRNKTVVAAWNGEIQTAPKPGASSAQPPSSSLAYATPDHPEYKRYPAH
                     DLLVVTPEDVERRGEQNTLAPTGDRHNHRTLPPPQISPIA"
     gene            complement(11466..13142)
                     /gene="ilvD"
                     /locus_tag="PL162_00410"
     CDS             complement(11466..13142)
                     /gene="ilvD"
                     /locus_tag="PL162_00410"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HQF90522.1"
                     /translation="MPSDTAKRGAPRAPHRALLKAAGYTDWEIERPWVGVVNAANAII
                     PGHTHLGRLTEAVKAGVYAAGGLPLEFPVIGVCDGLAMNHAGMRYSLPSRDLIADSVE
                     IMARAHALDALVLVTNCDKIVPGMAMAAARLDLPALILSGGPMLAGRHRGRPVDLSSV
                     FEAVGAHAAGRMDPHELEALAEDACPGCGSCAGMFTANTMNCLMEALGLALPGNGTIP
                     AVHAARERLAKDAGRQIMTLLERDLRPRRILTPGAFDNAVAVDLALGGSTNSALHLPA
                     IAAAAGITLPLDRFDDIGRRCPHLCAMSPGGPHHLEDLHAAGGVRGVMARLAEGGLLD
                     QRALTVTGLSVEENLHHTGEIDPRIIRPLANPVHAEGGIAVLRGNLAPEGAVVKQAAV
                     APEMRRHEGPARIFDGEEAASAAILGGEIRPGDVVVIRYEGPRGGPGMREMLTPTAAL
                     AGRGLDASVALVTDGRFSGATRGAAIGHVAPEAAAGGPLALVREQDRIRVDIPARRLD
                     LLVADDELARRRREGLPPPRPTESSLLERYRAAVSSAPRGAVLETPGAPS"
     gene            complement(13414..15105)
                     /gene="ilvB"
                     /locus_tag="PL162_00415"
     CDS             complement(13414..15105)
                     /gene="ilvB"
                     /locus_tag="PL162_00415"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HQF90523.1"
                     /translation="MTCTGAQMVVKALEAEGVPVVFGIPGGPIIPLYDALMDAPFRHI
                     LVRHEQAACHAADGYARVTGRPGVCIATSGPGATNVITGMATAAMDSVPLVVIAGQVA
                     RPLLGSDAFQEADIFGMTLSVVKHSFFVQHLAELPRALRGAFYLAASGRPGPVLVTIP
                     VDVLRASGPFFYPAEVTFPGYRPGAWEDLSRLDEAADLLRRAERPVFLVGGGVIRGEA
                     WDPLFRAATRHGVPVATTLMGKGAFPESSPLALGLLGMHGTPVANRALTEADLVVAVG
                     TRFSDRSTGQVARFAPRARVIHADLDPAELGKNVPVHLGLVGEARKILEALAAAPAAP
                     EEHRREWGATLESWRQKHPLRDPRRTDGYAPWDVLDAVRARAGEDALVTTEVGQHQMW
                     TALHWPLDHPRKFISSGGLGTMGFGLPAALGAHQGAPHRPLVCVAGDGSLLMNVQELD
                     TLVREGVPARIIVFNNGSLGMVRQWQKLFWKERFSATETGEGCRFAALAEAFGARGWT
                     VQTPQELGPALDGAFAARGPSLVDVRLPREEMVFPMVPAGEALENFIEAGAPSTR"
     gene            complement(15102..16262)
                     /locus_tag="PL162_00420"
     CDS             complement(15102..16262)
                     /locus_tag="PL162_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HQF90524.1"
                     /translation="MNSRKTHEKRTHRLACLPGDGIGIEVTAEARRVLEAAGARYGFA
                     LDWLDLPFGAAHYRASGEIFPDIALGELATCEALLLGAIGDPSVPPGILERGILLRLR
                     NHFDMYINLRPARSYPRVPLPVTLAEGTRLDALVVRENTEDFYVGLGGRLEEGKLHLD
                     TALERQTYTLRSRGSWELSPPGSGALQVGLATEGGVRRITAFACEAAQKRQESRLILA
                     SKANAMPQLYGFWEEVARDEARRHHMPLDVVNVDALCYHLVRSPEKYRTLLCPNMFGD
                     IVSDLLGALAGGLGLAAGANLGSGLGMFEPVHGSAPDIAGRGIANPLAAILSAGLLLE
                     SLNEPEAASAVHRAVERALEQSPRENLPRELGGHASTRAVGETVVACLEEGP"
     gene            complement(16259..16807)
                     /locus_tag="PL162_00425"
     CDS             complement(16259..16807)
                     /locus_tag="PL162_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HQF90525.1"
                     /translation="MREEKTPAPGGRWDGSAWCYGDDVDTDVIIPARYLVTSDPKLLG
                     PHCLEDLDPHFASRVTPGDVVVGGENFGCGSSREHAPLALKGAGVSCVVAASFARIFF
                     RNALNVGLPILICPEGARHTAPGDRLRIDLAQGTVENLTRGARYTGAPFPPFLQELVA
                     SGGIVPYIRRRLAQKKHQEARS"
     gene            complement(16800..17069)
                     /locus_tag="PL162_00430"
     CDS             complement(16800..17069)
                     /locus_tag="PL162_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90526.1"
                     /translation="MQHPFNPSGAGSCPFAHVGCGRSISPLSVSPTPQQKRVPLGGLF
                     CGPFGEPFRDLSFEERATPREEPQDIVFRDPFGFSLRDPWWDDVA"
     gene            complement(17182..18435)
                     /locus_tag="PL162_00435"
     CDS             complement(17182..18435)
                     /locus_tag="PL162_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HQF90527.1"
                     /translation="MKHTLPSAILASRSQTPVREGEICAVRVDFAFANDITAPPAIRA
                     FREMGAQGVFDPGRCAILPDHFTPNKDMAAAQQAKEARDFAREQGLRYWEVGRVGVEH
                     AFLPEQGLILPGDVVVGADSHTCTGGALGAFATGLGSTDLAAAWALGETWFLVPPTLR
                     VTVEGRPSPWVGGKDVVLALLERLGVQGARYKALEFSGEAFAHLTLDDRFTVANMAVE
                     CGAKAGVFVPDAPILAYAEARKARPFHPIYPDEGASYEAVVEIDAGSLEPLVAAPPSP
                     AAVRPAKTFADVTVDQVFIGSCTNGRLRDLELAAGLLKGRRVADSVRCVVIPASYEVF
                     KEALRRGYIATFVEAGAVVSTPSCGPCLGGHLGILAAGERCLATSNRNFTGRMGHPES
                     EVYLAGPLVAAATALTGRITDPREV"
     gene            complement(18416..19969)
                     /locus_tag="PL162_00440"
     CDS             complement(18416..19969)
                     /locus_tag="PL162_00440"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HQF90528.1"
                     /translation="MDDGMVRIFDTTLRDGEQAAGVSLSREEKLQIARQLAALRVNVI
                     EAGFPAASPGDLDAVAAIAAEVRTSTVAGLARAHRGDIEAAGKALRHAALPRIHTFIA
                     TSDIHMTHKLRMSRERVLEEVRRAVSHARELVDDVEFSAEDASRSDPAFLAEVFRVAV
                     SCGATTLNVPDTVGYADPGEFGALVRRLVDDVGAGPEVVWSVHCHDDLGLAVANSLAA
                     VQMGARQVECTVNGLGERAGNASLEEVVMALRTRRDRFALGTRVDTPRLCAASHLVSR
                     LTGVLVPPNKAVVGANAFAHESGIHQHGVMSFRGTYEVMTPEDVGAAGTKLVLGKHSG
                     RHAFRQRVESLGYSLSEAQMDEAFGKFKELADRKESVSDGDLEALAVGETLDVAPQRR
                     YTLDSYDVHAGTERAMASVTLREGDATYADAAAGNGPVDAAYAALRRILGLAPELVHY
                     NVTATSEASDAVGEATLVLRLGDMMARGRGASTDVVASSIKAYVSAVNRLYELAAAKG
                     VTLCETHVA"
     gene            complement(19986..20996)
                     /gene="ilvC"
                     /locus_tag="PL162_00445"
     CDS             complement(19986..20996)
                     /gene="ilvC"
                     /locus_tag="PL162_00445"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HQF90529.1"
                     /translation="MAKVYYDGDVRRDVLRGKTVAVLGYGSQGHAHAQNLRESGVSVV
                     VGLPETSRSRDRARSDGFEVLSGADAAARGDVVMFLIPDHLQADVWRRDVRENLRPGA
                     ALAFAHGFSVHFGQIEPGADTDVFMVAPKSPGHLVRRMYAEGKGVPALFAVYRDASGQ
                     ARDLALAYAAALGSGRAGMLETTFAEETETDLFGEQAVLCGGVTELVKAGFDTLTAAG
                     YQPEIAYFECLNELKLIVDMMFEGGLGWMRYSVSDTAKYGDCVAGPAVVDARVRATMK
                     ELLERVQNGTFAREWILENQAGRPRMKAWMTRERQHPIEQVGAELRRMMPWMEPKGAP
                     DV"
     gene            complement(21084..21617)
                     /gene="ilvN"
                     /locus_tag="PL162_00450"
     CDS             complement(21084..21617)
                     /gene="ilvN"
                     /locus_tag="PL162_00450"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HQF90530.1"
                     /translation="MRHTISVLAEDRPGVLARLAGLVSRRGYNVSSLSVGATEQPNFS
                     RFTLVVKAEAGAVRQMVHQLEKLVETVDVRHLASGNLVERQLFLVKVRAPLEQRAAVL
                     QTAGIFRCRVVDMGRDAVVFELTGDQGKGRAFLEAMRPFGVLEAVTSGAVALERVGFG
                     QDEELEDPFPSAAALTA"
     gene            complement(22479..23984)
                     /locus_tag="PL162_00455"
     CDS             complement(22479..23984)
                     /locus_tag="PL162_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQF90531.1"
                     /translation="MLTFSHVSFRYDTAAPEVFHNLTVAFAPGWTGVVGANGAGKTTL
                     LRLALGELEPRSGAVQGPRRRAWCPQRVELCPEELEPLLQSRDPRGARLRGILGLDPS
                     WPHRWHTLSPGERKRAQIAVALWQDPELLLLDEPTNHVDAAARALLGRALGTFRGVGL
                     LVSHDRELLDTLVTQCLFLDPPRGVLVPGSYRTASRVRQEERQAAETARDLQRREVKR
                     LEEEEKRRKEAARSADRRCTKRSLAKGDSDGRAMINLVRVSGKDGQAGRFQNQIEGRL
                     QRSRDTLKALEIRKSPPAHFWLSGARSSRNRILNISGFSLPLGEHRTLDIPCLSMGPS
                     DRVALVGPNGSGKSTLLGYLQDRWELPPHRILFLPQELSAEDEARTLRTLRALPPARR
                     GLVGTVVRCLGSDPAGIFAGGSASPGELRKILLALGVLEEPHLLVLDEPTNHLDLPAV
                     ELLEGALATCPCALLVVSHDEAFLSALTRIRWTLEPRGNRVELRGATSGGG"
     gene            24785..26638
                     /locus_tag="PL162_00460"
     CDS             24785..26638
                     /locus_tag="PL162_00460"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQF90532.1"
                     /translation="MRLSANVWRRFFLAAPFFLVVLFTAASCGSGGGGGTSYEKTMTQ
                     MTSFIEEQMEKDQVTGLGIALVDEDRTVWARGFGYADEAQQIPATKDTLFEIGSNSKT
                     MVAALVMQLVEAGRLHLNDPLEKHIPNFAMKKPLGSFPSPGGEVTIRYMLNHHSGLPG
                     DLFNGAFAVGKSDPKFNDYLVAYLAGEYGDFPPGFLLAYSNTAYSLLADVVAAASGQS
                     FQERSQAFFRDLGMDHTTFFRESAAVPPHQSKGYLMGQEYGPFYCNIPAAGSVLSSAS
                     DMAKYLKMVLAGGVGERNRVLSKETLSTMLTRTNGSVALDGDVSVGLGWFLSDDPLTY
                     AGRLCWHNGGTLLMMSHMEILRDHGLGVVVMTNSITGAAVAHDAAHKALQLALEEKKG
                     LRPPKAPDPDPSPVVTWTDAQLDAVAGIYVTTTTGYDRLVREDGGLRWVENAHGAAAR
                     DGSGDVLGETGRRLVPRANGWFSSPDSQGEQFEFVEMEGRRVMISHTSTFKGLLAERY
                     EPPADFSDAWKARRGIYDLVNLREDDASRALPPEYGFNSFTAEFDIQDGLLVLELTQG
                     LRKGMRYVIHPLSDTLGYLRGLGRNLGSSVQIISTGAGEELQLLGLRYRKR"
     gene            complement(26840..27100)
                     /locus_tag="PL162_00465"
     CDS             complement(26840..27100)
                     /locus_tag="PL162_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90533.1"
                     /translation="MPLLLFAFGAARIPLITVGLLQYVSPSLSFALGVLVYGEVFDVT
                     PGVTFGAIWLGLGLYTPDAFLWSQRRPSPAPQEEPPSPQKGA"
     gene            complement(27294..27506)
                     /locus_tag="PL162_00470"
     CDS             complement(27294..27506)
                     /locus_tag="PL162_00470"
                     /inference="COORDINATES: protein motif:HMM:NF022597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HQF90534.1"
                     /translation="MAKVLKLTFTCADSKDWTLSLKYPKDGLTLAQANTCMDAIIAQE
                     IFLSAPVTKKGAEIVDPCPGFPSCSP"
     gene            complement(27544..27762)
                     /locus_tag="PL162_00475"
     CDS             complement(27544..27762)
                     /locus_tag="PL162_00475"
                     /inference="COORDINATES: protein motif:HMM:NF019487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HQF90535.1"
                     /translation="MATYTSAGSSFALRVQVGVKPSGMPDVKSMTLSGIDAGATANAM
                     YAVGNAIGAVLAHSLVEMIKNDKDLVVA"
     gene            complement(27975..28304)
                     /locus_tag="PL162_00480"
     CDS             complement(27975..28304)
                     /locus_tag="PL162_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90536.1"
                     /translation="MMWPTTKIEVVRDLFPNVVQAFEDFLAQGGECDMEAQPADPGID
                     REMKVYAKAFHRPFPIDYMQNYLSAEEAIAHIRSFIERGKPVELSDLGVPLDVAIEWG
                     FNPKRYL"
     gene            28699..31677
                     /locus_tag="PL162_00485"
     CDS             28699..31677
                     /locus_tag="PL162_00485"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HQF90537.1"
                     /translation="MIESQIEDHLIEKLTDLKYTYRSDIHDRAALERNFRGHFEALNR
                     VHLTDSEFGRLLERIITPDVFAAARTLRERNTFERDDGTPLHYTLVNIKDWCKNTFEV
                     VHQVRISTDYSHHRYDVILLINGVPAVQIELKTLQISPRKAMQQIVEYKNDPGNGYGK
                     TLLCFIQLFIVSNRTDTWYFANNNHRHFSFDADERFLPLYQFADPRNAKITHLDSFAE
                     AFLAKCTLGEMISRYMVLVACEQKLLMMRPYQIYAVKAIVDSISQNCGNGYIWHTTGS
                     GKTLTSFKASTLLKDNPDIDKCLFVVDRKDLDRQTREEFNKFQEGCVEENTHTESLVR
                     RLLSEDYADKVIVTTIQKLGLALDGNNSRRNYAERLEPLRSKRMVFIFDECHRSQFGD
                     NHKAIKAFFPRAQLFGFTGTPIFEENATHQQIEGQQASYRTTVDVFRQQLHAYTITHA
                     IEDRNVLRFHVDYFKPDGKAPRGADGSLTKKAIVEAILAKHDAATFGRRFNALLATAS
                     INDAIEYHALFKTVQAEKAAADERFSPLHVACVFSPPAEGNKDVQQIQEDLPQERIDN
                     EQEPDAKKEALEGIIASYNARYGTNHRLGEFDAYYQDVQKRIKDQQYPNSDFPHKEKI
                     DVTIVVDMLLTGFDSKYLNTLYVDKNLKHHGLIQAFSRTNRVLNDTKPYGHILDFRQQ
                     QDAVDTAIALFSGETAKPAKEIWLVEQASVVIQKLDDAMEKLGQFMRSQGLENTPEAV
                     LHLKGDDARTSFINQFKEVQRLKTQLDQYTDLTDEHKQKIEQVLPKDQLQAFKGVYLD
                     TVQRIRDERGKGGVDKKPEEGGGGQLDPELVLFASADIDYDYIMALIATYSQQAPDKQ
                     KMTREQLIGLIQADAKFLDDRDDIAEYIGTLEQGTGLDETAVRAGYQRFKEEKHAREV
                     AAVAERHGIEPAALRAFVEDVLRRMVFDGDRLGDLFALQELGWKARAQKEQDLMEDLL
                     PFLKKLAQGREISGLGAYEQ"
     gene            31667..32989
                     /locus_tag="PL162_00490"
     CDS             31667..32989
                     /locus_tag="PL162_00490"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HQF90538.1"
                     /translation="MSSKPEEAGTTAKAGRPLVPTLRFPEFRDAGEWETLPMRKLLSR
                     DPEYGVNAPSSPYSEDKLTYLRITDIDDDGNFLSNSKVSVDINPVDGNFLKKGDIVLA
                     RTGASVGKSYRYKDKDGPLVFAGFLIRIRPESTKAVSAFLSNFLTTQQYWSWVGVTST
                     RSGQPGINGTEYASLPVPVPPAIAEQQKIADCLSSVDDLIALEARKLDALKTHKKGLM
                     QQLFPAEGETLPKLRFPEFRDAGEWETLPMRKLLSRDPEYGVNAPSSPYSEDKLTYLR
                     ITDIDDDGNFLSNSKVSVDINPVDGNFLKKGDIVLARTGASVGKSYRYKDKDGPLVFA
                     GFLIRIRPESTKAVSAFLSNFLTTQQYWSWVGVTSTRSGQPGINGTEYASLPVPVPPA
                     IAEQQKIADCLSSLDDLIALETQKLDALKTHKKGLMQQLFPVSDEVPA"
     gene            32986..34002
                     /locus_tag="PL162_00495"
     CDS             32986..34002
                     /locus_tag="PL162_00495"
                     /inference="COORDINATES: protein motif:HMM:NF024706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HQF90539.1"
                     /translation="MTQLILYTSEDGHAHVQLRAHRGTVWLSQRQMAELFDVSPDTIG
                     LHLKNIYADGELQRAATAEESSVVQTEGGREVRRSVTLYNLDAILAVGYRVRSPRGVQ
                     FRRWASTVLAEYLRKGFVMDDERLKNPDGRPDYFDEMLARIRDIRASEKRFYQKVRDL
                     FALAADYDKTDQATQTFFAAVQNMLLYAVTQCTAAEMVLHRANPADPHFGLRAWTGHR
                     VRRHDILVAKNYLTEEEIDTLNRLVVIFLETAELRAKRQTVTTMAFWRENVAQIIVAN
                     GFPLLAGAGSVSHARVESHVTPLFLDYEQRRKTQEAQEADAQDEAELKALEHRIKDRK
                     EAES"
     gene            33999..35603
                     /locus_tag="PL162_00500"
     CDS             33999..35603
                     /locus_tag="PL162_00500"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HQF90540.1"
                     /translation="MIEQDKRKLGATLWAIADQLRGAMNADDFRDYMLSFLFLRYLSD
                     NYDGAAQKELMSDYPKLPPGDRRPPLAVWYDQNPGDVDEFEKQMRRKAHYVIKPEYLW
                     GSIAEMARIQNGELLHTLEKGFSYIENESFDSTFAGLFSEINLNSEKLGKDYVARNAK
                     LCTIIGEIAEGLAQFSADSDTLGDAYEYLIGQFAAGSGKKAGEFYTPQQISSILSGIV
                     TLDSQEPATGRRKNLESVFDFACGSGSLLLNVRRRMGTMGIGKIYGQEKNITTYNLAR
                     MNMLLHGVKDSEFQIYHGDTLTNDWDALRETNPAKMPKFDAVVANPPFSYRWEPTEAL
                     GDDMRFKNHGLAPKSAADFAFLLHGFHYLKPEGVMAIILPHGVLFRGGAEERIRTKLL
                     QDGHIDTVIGLPANLFFSTSIPVCVLVLKKCKKPDDVLFINAADHFEKGKRQNVLTPE
                     HIEKILDTYQHRREEERYARRVSMKEIEENGYNLNITRYISTQEAEDQIDLRAVNAEL
                     VGVTQKIEEARERHNAFLRELGLPPV"
     gene            35732..36115
                     /locus_tag="PL162_00505"
     CDS             35732..36115
                     /locus_tag="PL162_00505"
                     /inference="COORDINATES: protein motif:HMM:NF016178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HQF90541.1"
                     /translation="MKALSVRQPYATLIATGEKTIEWRSWKTPHRGALMICSSASWAE
                     GEWDIPKEDRDQFPRGVTLCIVDLVDCRPFKKKDLEAAFMEENPGGYAWVLANPVPIA
                     PRPIKGKLHLFDVPAPSEAGAEELC"
     gene            complement(36179..36454)
                     /locus_tag="PL162_00510"
     CDS             complement(36179..36454)
                     /locus_tag="PL162_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90542.1"
                     /translation="MNVKDSLVWEEWDEELERRKEEGEMDVSLMGKKGFSKGRAEGWA
                     EGRMEGEIDTARRMLALGESDEKILYVTEITPEQLEALRREGTLSSS"
     gene            36586..38721
                     /locus_tag="PL162_00515"
     CDS             36586..38721
                     /locus_tag="PL162_00515"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQF90543.1"
                     /translation="MRCFIKRCSLRTQIMGGTAVIVVGALAVSFWGSYLTARDRLLES
                     SVTQGQGAVTSLAHATENTLKLLRTACATNLDVSAGTVAGGRLMPSRFTVTMGKWVLT
                     PLSLSTGNLVKITGNNALPEEWAARMGGCFSLLQPASADGAVALVRVSTSIKDASGQS
                     LIGTPLEDDSPATPALARGESFFGPTEIHGEPFFGGYRSVKGKTPEEHVVVFAGVSLT
                     PLLQFLRESHIGEGSEVRVFDAHGRELGKGASPEGSSSLWAALEPWRAKIAPGEVESF
                     SEGEGKAARQVFVFRTDSPEWTVAVTIPSSVITAPLVAMRERMLIWGLLSLGAGLALV
                     TFLVGRLLAPLRNVVETAHRVAIGDLRVSTCADEDEGSRNEICVVLSAFARIGTAYRD
                     LVSQMQGLQELFQERGDAMEHINGEVVASLREALGAAQDMVRTVERVVQGADGTKSRV
                     TSVTQASREASSLVAGLSRESEEVARETSANGEAVRAVAVGIREAGGASVRVRDALGA
                     LESSVESISRFVATIQGIADQTNLLALNAAIEAARAGEAGRGFAVVAEEVRKLAEDSN
                     GASRQIQSVIEEIRARTDETVRDTERVTELIAGTEKDSQETAERIGEIVAHVSAIAEG
                     IRSIAAVTQNQLAGNEAVENAMDMIHRQAGEGQRAASRVEQVSRQVAGQMDHLEEIRR
                     QQGDVLEDLGRALAGYVLDDASEVRALEE"
     gene            complement(38844..39770)
                     /gene="rarD"
                     /locus_tag="PL162_00520"
     CDS             complement(38844..39770)
                     /gene="rarD"
                     /locus_tag="PL162_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HQF90544.1"
                     /translation="MSTQRASEFQAGFWATLGAFLLWGALPVYWKALEEVPPLEILCH
                     RILWSMLVLLIMLSATRRWGEVWSALSRRSVALGLAVSGTLIGVNWLAYIWAVNTGRI
                     LESSLGYFITPLVNVLFGMIFFHERLRPYQWAAVALAALGVGYQLVLHGSLPWVSLFL
                     AVTFGSYGLMRKKLPVGSIPGLFVETLLLSAPALAYLGFLALQGQQCFGAGSLFRDAL
                     LSGSGIITSVPLLLFAFGAARIPLVTVGLLQYVAPSLSFALGVLAYGETFDFTRGVTF
                     GAIWIGLGLYTLDSFLLSRRSSPEASSLRREA"
     gene            40269..40856
                     /locus_tag="PL162_00525"
     CDS             40269..40856
                     /locus_tag="PL162_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90545.1"
                     /translation="MAKGRGLGPWILGVVLILAIPAWGVDIGRWDGPDLGLGLSRALV
                     RYLLGSPDAVAGRTWVYVVSGSREIDLGFVDFSSGDMVCVAAQRYRPGGVSAVSLVEL
                     LQRQGFVSVVADGHRWLLTGEDGSSGQAAFFLVLSPDLAESPSRGPLLATLDERPWRA
                     LLEGEDRGRTAWERRFVAMMAWPRPREGESRDGAP"
     gene            40867..45108
                     /locus_tag="PL162_00530"
     CDS             40867..45108
                     /locus_tag="PL162_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQF90546.1"
                     /translation="MESLRRWLFLVVVLWGALMGGADGALALARVFRFDHLGVGQGLP
                     SGVVQALLQDRTGFLWIGTRGGLCRYDGRETLSVPLERPGEPRRRMIRALAEDGEGYL
                     WVGTGGDGLVRRHQASGELTVFGRGLPGGLGGDKVFSLLVDARGVLWVGTEGGLARYD
                     RVHGVFEPFPRPGKDALGLPSAPVRALCEDVRGRFWVGTDEGLALVDRGTGEVRLFRH
                     GTGDPRSLGRNDVRALCADGDGALWVGTGGAGLDRLDPSGEFFIHHRPDPQAPGRLGG
                     GAVRALCRDGRGNVWVGTWEGGLFRFDREKGRFEGFRHTPGRAGSLGRNTVNCLLVDR
                     SGLLWIGLEGGGAASLNLRAAENFPLYTHDPGDPSGLASSMVLALGRDSSGAVWVGTD
                     RGLDRIDRSWKSVRSFRPDPQRPDSLAGESVRAVLADSRGFLWVGTAGGGLQRLYLPA
                     LDRGGFFAPLRRDPPFRRFYHDPSRENSLADADVTVLLEDREGLLWVGTAGEGLDCFD
                     PRKETFTHHRYDPDALFSLGGNTVTCLLEDRFSHLWVGTEGTGLARYDRSTDRFVRLR
                     EDHGTPSVGTPTCLLEDRRGRIWVGTEGGLRRLDRARGVLVEVPEVRPPGEDAVTGML
                     EDDTGSLWIATRGGLCRYWPETGRFEAYGAREGLQGEEFNYGACVRGSEGELIFGGSG
                     GLNAIRPGWLRRNLMPPGVAITGVAVEGSPLDPMTLGSLAPVSLGPRDRSVTFTLAAL
                     DFSSPERNRFSVKLEGFETSWVSLGFRNVVTYTNLDPGSYALWVKAANADGVESPPER
                     LIRLTVPVPGWRSWWAISLYALGSLGILGGYGSFRVRREERVLREQRERARALEEEVA
                     LRTRELHEKNEALRRSAQMLQMEVEERLLAQDALVAAKDAAESASRAKGVFLANVGHE
                     LRTPMNAILGFTDLLLEGEISPTQRPWLVTVRSAAGSLLRLLNDLLDLSKAEADRLDL
                     EEVPFDPAAVLEEVRSLLEPRAREKGLAFCTEVDASVPPILRGDPFRLRQILTNLVDN
                     SVKFTAAGHVAVFLTAQPEGGRVWLRGRVEDSGVGISPDQVGRLFEPFSQGDSSTSRR
                     YGGSGLGLAIVRRLVDLMEGSLEVAPREEGGSIFSFSLSLGREEGGGEGKTHKEGSPE
                     LEGEARALPLGLRVLVAEDNPPNRRFLEALLGRYQWDLTFVEDGEEAWRLLAPSGEGS
                     LGSPFDLALLDVQMPGKDGPEVARLLREAPGGDRLPLVALSAGAGEGERRRCLASGMD
                     AFAPKPLTRRALEGAVRAALGRRGKGGDFFEARDPVVGSWGELFEAYEGQEGELRRLL
                     EDFRGSLPGVLRALEAGVGKEDRDFLLRRIHRLKGSLGAFWMERSGALAREVEGALKR
                     GDDEGARRGLALLALRSEEEGRALGRALEDRRGTR"
     gene            45105..46919
                     /locus_tag="PL162_00535"
     CDS             45105..46919
                     /locus_tag="PL162_00535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQF90547.1"
                     /translation="MTQGDRRRLLLVDDDDVFRKTTATRLRHLGYEVREASEGEEALG
                     AVSADLDLVLLDLGLPDRDGVEVLGVLSSAWPELPLLVVSGAEEVGTAVEALRGGAWD
                     YLLKPLGDRESLSHALERAFERRDLLRENRAHREHLEEQVADRTRRLQEELAERSRVE
                     EELRRSQEDLRSLASELSLAEERERRRVAVDLHDGVGQTLALLKMKFSHLRRTAGEAL
                     PSKPFDEIGALVEEAIARVRALTLEVSSPVLYEVGFEAAVSALAGKMLDEHGIAWTLH
                     DDWQPKPLAEDVKVLLFQVVRELLHNVVKHAEARRVTLSLRREGRELVCTVADDGKGG
                     VREEERPQGGGFGLFSIRERLFHLGARFELHSSPGEGTTGILRAPLLIPREASRKEEA
                     RPPIRVLLADDHDLFRRGVRALLEPYADLDLVGEAEDGEAALALARECHPDVVLLDVS
                     MGRHGGIEAARRFSEEFPKTAILGLSMHANPFVVREMLDAGGAGYLLKDCDPEELARA
                     IRAVAAGEAAFSAPVAAAVAEDLVRQRRRGQEALSVREEEVLRRLAEGESIKEIAAAL
                     GISAKTVETYRERIGVKLGLHTVVDMTKYALRKGIVAL"
     gene            46951..48315
                     /gene="miaB"
                     /locus_tag="PL162_00540"
     CDS             46951..48315
                     /gene="miaB"
                     /locus_tag="PL162_00540"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HQF90548.1"
                     /translation="MASFVLKIFGCQMNVYDGDRLRTALEAGGWREGEEESADAVIFL
                     TCSIRDKAEQKVWSALGRYGERWRGQGRPTVAVLGCMAQRVGGELFRRFPWVRVVSGP
                     RHLGRIPEALESALSERRRHLLLDEDPRALEDLCAPPLRRENPYRAFVSIAHGCDNFC
                     AYCIVPHVRGRFASRDPGAILDEVRCLVASGVKDVTLVGQNVNSYGTDRVDGWTFPRL
                     LREAGRVRGLWRLRFATNHPKDLTEELGRTLAEGPPLCPALNLPIQAGSDRVLALMNR
                     GYTVEDYALRVAMLRRYLPDVGLTSDLIVGFPGETEEDFRESLGALERFRFDQVHTAA
                     YSPRPGTQAASMAPAVPEEERFRRLEEANSLQRRIATEINRDRVGRVYEVLLDAPAPR
                     GEGYLQGRTPQDKVVLVRGEVALLGTMGAVRITAGEPWGLRGEWANEGPPGDAGGVEG
                     EEKA"
     gene            48312..48941
                     /locus_tag="PL162_00545"
     CDS             48312..48941
                     /locus_tag="PL162_00545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HQF90549.1"
                     /translation="MNLSPKTRQMLVLAGGILCLLVAWILVGIFAGRWKETAPTSMAG
                     PALSTKDPLPTSPPLTELVVYLTGAVRRPGVFSVPPGSRLVAVVEKAGGLAPEADAER
                     INLAQPVADGAHLHIPRRGDRERLAALAPPGGGAPTSPEDPGRPVDVNSASAEELEKL
                     PGIGPKTAQAVVEDRERNGPFRSVDDLGRVKGIGPKKLEALRPLVTAGP"
     gene            48945..50357
                     /locus_tag="PL162_00550"
     CDS             48945..50357
                     /locus_tag="PL162_00550"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HQF90550.1"
                     /translation="MLGIMPSMPIFLGWCAALGALGGGVPPLGAWGVGMLVTAGAIWS
                     TALAFPRNFWAVGSAALLLGALGGAAVVYRGAFPERPEGKIEGEGLVTAERPWGGRRA
                     VLVTLPQGTFLAFSSPVTGLREGDRVAVTGTLEAFRRSPGGSFDAERYWRARGAKGEL
                     RGASWTLLPPERWSLAAVRSSLRKKILLTLPPLTRGYLLAALVGVSDPDRAAVHRRAG
                     TVHLLSVSGFHVGLVGLFGALIFRGRGRWVLSSVLIWVYVALAGAPPSAVRSAVMAQG
                     ALLGWTLGRRGGITNALAVAGGGMLLWNPWYFWDVGWRLSVLATLALALLAESRLPHR
                     VAGILASPGAWIATAGECAAVFGPVPLAGLAINLVALPAFGVLFPVGILAGLPSLLEL
                     PGGWVGADFAEDCFVLWEGLSDALVRLFPGSVTDAWIPTGIGALALGTLGGRVLGLPP
                     GRTLFLGAATMAFWGMIKLI"
     gene            complement(50386..51312)
                     /locus_tag="PL162_00555"
     CDS             complement(50386..51312)
                     /locus_tag="PL162_00555"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQF90551.1"
                     /translation="MRCKAIVVFVALGAILLAKAEGRAAPSGAASGGARPLSTDAFER
                     HVVQEGETLFAVARRYHTSVTALARINGLEDISRLAMGQVLLVPRGATSPDLPPRPEV
                     VPSARDPQDFPAPRPATHTVQGGDTLFAVARRYQVSVDLLLELNALPDPQHLTPGQVL
                     RVAPLPRPSGAPGRPSPHLSVASQTPGEEALAWPLRGAVLRRFGQEGSPSLHLEAPEG
                     TPIRAAAAGIVLAAGWMKGFGNAVYLQHADGIATFYGNCGVFYCKKGAVVRRGDRLAA
                     VGPAPVSGPAQLAFTVLKNGKAVDPLPLLPPP"
     gene            51470..52234
                     /locus_tag="PL162_00560"
     CDS             51470..52234
                     /locus_tag="PL162_00560"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HQF90552.1"
                     /translation="MLLVLDIGNTTTVLGLYREDSLVRSWRLTSEKRTADELSVYLLT
                     LLGTLRVDPEEITGAAMASVVPPLDAPWSEAVTALCGVHPLEVTSSLDLGMPVCLLNP
                     GEVGADRLVNAVAGMAAYGKPLLVVDFGTAVNIDVVDGEGRYRGGALAPGLAVSMEAL
                     FGRTAKVPKVALAPPPFAIGRSTQEAVQSGIIFGYAGLVDALVRRMWKELGAEAPVVA
                     TGGQASLVASFSETIRSVAPDLTLEGLRLIHRRHRG"
     gene            52371..53948
                     /locus_tag="PL162_00565"
     CDS             52371..53948
                     /locus_tag="PL162_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HQF90553.1"
                     /translation="MRKNRRILALCVVALLVLVLGSVAEAARLTVLHVNDTHGHAWPF
                     DLYNNPGVGGFAPLATLIQEIRQEVEAQGGHVLLLHAGDFNTGVPESDQNDAMPDIVA
                     MNMLRFDAVGLGNHEFDKPREVLDRQGRFLDCPLVNANILTGEGKQAFAPYILKDFGD
                     VKVGLYGLTTEETSKLEPTYLGDWKFENAVETSKRIVGELKKSADVVIALAHLGWAEA
                     PAAGYTTSKMLAEASVPGLDVIVDGHSHTIFEQPPLIGTTMVVSAGEWTQYLGRLDLV
                     VEQGTIVGRTWRLIPINAKTVTGKDAEGKDVLGFVDKEIPADKEVEAALGYFLKIGAE
                     KLALVVGETKVLLDGERNNVRARDTNLSNMVADSMRWKTGADIALMNGGGVRASIQPG
                     PISYRDILTVMPFGNTLYVMEVTGEEIQKLLDFVVTIPEGKGGWPHFSNLTFAAKDGK
                     GVDAKVGGEPLDPKRSYKMVTINYLAMGGDGYEVFRDAVARGGYDTGFVDAAAFQEFL
                     ADQKVIENWDDSQRYVR"
     gene            54021..55058
                     /locus_tag="PL162_00570"
     CDS             54021..55058
                     /locus_tag="PL162_00570"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HQF90554.1"
                     /translation="MKNTMPPGGQEVGSVAVANPLWLAPLAEISVPAVRALFRRMGVG
                     LTHTEMVSSSGLCHGNRATLAMLPRRGEAPVVLQLFGDEAEEMGRSLEIALIRGERPQ
                     AVQINMACPVRKVFRRGEGARLLERPAVAEAMVRALVPFGLPVWVKTRLAGRGCPLST
                     GDFCDVLFSAGASCVALHGRTAAQMYEGTGDRDAVGAVAARFPGRIVGSGDVYAPEDV
                     QDYLSRGCAAVLLARGGVRDVYSTPKILQALGYGGAPEVLNPTPAQRVATLMNLADDM
                     AEEVGERGAMILLRRFLSGIFKGLPGATFLRRSIGTVTDRPSLESVVRRWEQVLVEGG
                     TDHGGSGTVAG"
     gene            55030..56553
                     /gene="lysS"
                     /locus_tag="PL162_00575"
     CDS             55030..56553
                     /gene="lysS"
                     /locus_tag="PL162_00575"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HQF90555.1"
                     /translation="MEDQERLQAEGGSQVPEEEILAARKEKLRRLREEEGYDPYRPER
                     FERSAHLAEIKRRFDSLTVDQHGTEKLTTAGRVMALRKHGKASFITLEDEGDRMQLYF
                     QFDALGEEAYTFFKKWVDVGDFLGVEGHPFRTSRGELSVQVESFSLLSKALRPMPEKW
                     HGLKDVEVRYRQRYVDLLANRGVRDTFRKRATIMAAIRALMTRTGSLEVDTPILSHLA
                     GGANARPFVTFHNALGLQMYMRIATELYLKRLIVGMMGRVYEIGKNFRNEGVDTMHNP
                     EFTAMEVYWAYADYEDMMTLCENIVVAAADSVGGRTVTYQGKEISLHPPFRRATMKEL
                     VKEHAGVDFDAITTDEEARRAARAKGIPVEGHESRFLLLAKLFEEYGEKHLIQPTFVL
                     QHPVEISPLAKRNPEAPDYTNRFELFINGSEVANAFSELNDPLDQEERFRDQQRKKDA
                     GDEESHPFDEDFVTALEYGLPPTGGLGVGIDRLVMFLTDSRSIRDVILFPAMRPREA"
     gene            56623..58674
                     /gene="ligA"
                     /locus_tag="PL162_00580"
     CDS             56623..58674
                     /gene="ligA"
                     /locus_tag="PL162_00580"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HQF90556.1"
                     /translation="MNDEKELRRRVEALRREIIHHAHRYYVLDAPEIADDAYDALMIE
                     LLALERDHPELQDFGSPTARVGGAPREGFVKVSHPEPMQSLDNVFSLEGLGDFLDRQR
                     RILPDLSGGYVCELKIDGLAVSLHYENGVFVRGATRGDGTVGEDVTANLRTVRTVPLT
                     LRESLPGALEVRGEIYMRSEDFAALNAEREEAEEPLFANPRNAAAGALRQLDPAVTAS
                     RRLSFFAYAVSFPERRGLATQRELLEALRRWGFATQGNEVLASNLREVGAYVERWREG
                     RFSLPYATDGVVVKVDRLELRDLLGSTARAPRWAVAYKYPPEEKTTRVRDIVISVGRT
                     GSLTPVAILDPVPLAGTVVQRASLHNADEVARKDVRIGDRVVVRKAGEIIPEVVRVVS
                     EDRSGTEIPFVMPPRCPVCGTPAVRLPEEAATRCPRRSCPAQIKEGLRHFASRGGLDI
                     RGLGEKIVDRLVETGRVKDLADLFDLSAAELASLDRLGEKSAANMIQALETSLGRPLP
                     RLLTALGIRHVGSRVAELLADRFRSVEALTRATEEELASVDGVGPRIAASVRAFLDSP
                     ANQETLRRLASRGMALALPEPEAPLAEGPWKEERVVFTGELRTLTRTEGEALVASLGG
                     RALGSVSSKTTLVVAGENSGSKLRKAQELGIAVVDEEEFLARVRRARGEKSPGPRDGA
                     S"
     gene            58830..59687
                     /locus_tag="PL162_00585"
     CDS             58830..59687
                     /locus_tag="PL162_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQF90557.1"
                     /translation="MSGRAYALALSAIGVWSSLAFLGLRLASVPPFLLVGLALLIGSG
                     CSVHHFRQWRVSPKLLALGVYGLFGYHLCFFLALRLAPPVEANLMNYLWPLFTVIFSP
                     WILPGYSLRPHHLGAAFLGMTGAALIVTGGSFVSFRSEFLPGYLLGGGAAFIWATYSL
                     TTKRLGGFPNAAIGGFCFASGVLSLLVHVLLEEPYIPTLGELPSLVALGLGPMGGAFF
                     LWDGALRQGDPRIIGSLAYLTPLLSTLLLTFSGRGEFTPIAAGAMGCILGGAFLGTWG
                     TRGKGAPSP"
     gene            59772..60164
                     /gene="gatC"
                     /locus_tag="PL162_00590"
     CDS             59772..60164
                     /gene="gatC"
                     /locus_tag="PL162_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HQF90558.1"
                     /translation="MHGRSEPEGIVSPEEVRRIAALIQLEVGDDEAEDLGRHFQKILG
                     YFARLREMDLEGVEPFTPGEPSIALREDVETPWEERGEVLDRAPFREGDFFRVPRMER
                     DEESRREGEGRAGSGEGGHLPPGSCSAD"
     gene            60177..61652
                     /gene="gatA"
                     /locus_tag="PL162_00595"
     CDS             60177..61652
                     /gene="gatA"
                     /locus_tag="PL162_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HQF90559.1"
                     /translation="MELWEYSAWRLAEFVAQGQLSVSEVVEAHLTHMQWQEGTYHALI
                     TPMVEAARDRARALDTALAGGADISPLCGVPVVIKDNICVQGSPTTCGSRMLERWVAP
                     YNATAVDHLLGAGAVVVGKGNMDEFAMGSSTENSFFGPTKNPRDPNRVPGGSSGGSAA
                     AVAAGYAPLALGSDTGGSIRQPAAFCGVHGLKPTYGMVSRHGLVAYASSFDQIGPLAR
                     DVGDLAVALEVLGRADDRDPTCSSRPRTTYLNAIQTYSLKGRRIGMVREFAGSAVDPE
                     LHAAMVHAARICQDAGATLVDVSLRDTIAYGLAAYYILAPAEASSNLSRFDGVRFGFC
                     REAEVLQEQYRRTRGEGLGPEVKRRILTGTYVLSSGYYDAYYATAQKVRRRVAEEFDV
                     AFQSADVVLLPTSPRLPFYFGEVGDPMAMYLMDIFTVPANLAGLPALSLATGSGRDGL
                     PLSVQLVGRPWGEFDLLGVASILEKAFGAPQPIQGTGGKYS"
     gene            61649..63115
                     /gene="gatB"
                     /locus_tag="PL162_00600"
     CDS             61649..63115
                     /gene="gatB"
                     /locus_tag="PL162_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HQF90560.1"
                     /translation="MREELSPVIGLEIHVQLATGTKIFCGCSTDYIGAVPNVNVCPVC
                     LGLPGALPSLNARAVELGTLAGLALGCRIRLRSRFHRKNYFYPDLPKGYQISQYDEPL
                     AEEGFIDVADEKGGTRRIRILRLHLEEDAGKLVHGAGDGRLEGASCSLVDCNRAGVPL
                     AEIVSAPDLTSPAMSREYVLALRRLVRHLGVGDGDMEAGSLRVDANISLKGPGESGGE
                     RVEIKNLNSLRALERSLEYEISRQRDLISRGGTVARETRHWNEVTERTLGTRSKEEAQ
                     DYRYFPDPDLPPLVITEGFVEACRRRLPELPDARRRRFVEGLGLSEEEAAVLVEERGV
                     AAYFDAAVAAGAPPRRASAWVRTEVLRVLREERRAVGAFPVLPDTLAALLQRVESGEL
                     STTVARECFHALRTEEISLDEALRRFGGGKPSSEELEALGRRTLEENVDVVEEILGGR
                     DPKGKKIKYLEGLMMRALRGQETPEAVRRTLEELIFKK"
     gene            63169..63834
                     /locus_tag="PL162_00605"
     CDS             63169..63834
                     /locus_tag="PL162_00605"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HQF90561.1"
                     /translation="MIQNVIFDLGGVLLKWEPLEYVHRTLGGGALAERICRGVLEAPE
                     WQDLDRGALEEDEALERFLQRLPEDKEAVRRIFPGLFEELRPIEKHVALLPLLKASGK
                     RLFVLSNFSRRGYAFVSSRYDFFRHFDGGVVSYEVKLLKPEREIYEALIDRYALDPST
                     CLFFDDREENVAAALDQGIDAVVVRHESQDHRGMAWPEVASDRELPGTFDLREEMGRR
                     GLL"
     gene            63974..66139
                     /locus_tag="PL162_00610"
     CDS             63974..66139
                     /locus_tag="PL162_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HQF90562.1"
                     /translation="MQDISVSTVAVVIQVLLYASVAYIALKRFKFPYTIGLVLIGLII
                     GAVVNIFGILPAFSRLHLTGNLILYALLPALIFDAALNMDVQLLKKNIVPVMVLAVPG
                     LVVATLVSAWLVAAWTPLGMGAALVFGALISTTDPVAVIALFHELGAPKRLTMLVDGE
                     SLFNDATAIVMFAIVLGLVSSGAPFSVGTVLLAAVEFFLVFIGGLMVGVAVGWVVMGS
                     MRFVRADPLVQLTLTIVSAYLSFLLAQGALGLSGVMASVGAGLVTAHYGRVYFSRETR
                     DYLHHFWSFSSFAANSFVFLVIGLTEVYLSRGHGFGATLFYALAAIAAITLARILIVG
                     ASFGILNLTRNFHRVNWREQVVVVLGGLRGALPIALAVSILPAEVGGEENRRLIVDFT
                     LAVVLFSLLVQGTTMGLVLRRLGLMKEDLVGRMVRLRSAIGAWRRTARALSSTVEGEE
                     TSVPLFDEVRRQYEAGVQRIRGELQNIGEESPEEARRVASALTWRYVLAWMRSSVEFM
                     HSAGVLDDPSALAIYLQIEEAADRNDHGETPTLAFRELEASGIQRAFLALLRRHAPDS
                     EFFRNSQEERTLFMSLLRRGAPRSLVYGELRREAERSPLRHALGCMAVAVRARLRVAE
                     VQEMVEMDPEVGEPLMAALQGFAEEARAFAQRYQETHPDHADEVVWNAMERYAAALAR
                     GAVGELKGWGMIDAAGAALLESMPSPFSVEEARAGAERG"
     gene            66325..67962
                     /locus_tag="PL162_00615"
     CDS             66325..67962
                     /locus_tag="PL162_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQF90563.1"
                     /translation="MVRGFRAVFLVMALILAVGGGAWAEDPVKGGTLLWRDPSDVPTL
                     DPAKVTDNSSTRLALTIFETLVENDLEGKGVLPCLAERWEGSDGGKVWTFHLRPGVRF
                     QAQAEGQPTANGGREVRASDVKYSIERLILTDSPRAYFLDMVKGYQDFMSRDVTGVTE
                     WAGVKVIDEKTVQFELDYPFAPFVAMLAYPTFSVVPQEDAEKWGKDFGFHPVGTGAFA
                     FEKWEHDIRISLKKNPDYWRTDARGERLPYMDGVEFVVVPDDAVAYEELKKGNLDVFS
                     YVPDEIYQDAREKFSDTLKESPALAVSYYGFNMAKEPFGSNKALRQAVNYAIDKNKLN
                     ELIYENRVVPAVGVLPPGMPGYDVTDMKGYPYDLDKARALMKEAGYEKGLECTLQINN
                     VQRHQRIGEAVQAMLMEVGITMNIVTVDWGTHIDALDRGEVPFFRYSWIADYADPDNF
                     LYVLFHSANAGPKGNNCFYNNAEVDRMLDQARVEVDWNKRAALYHEAEKIIVDDAPWV
                     FFFNPVNELLSVPRVKNIKLPTFGADWAPLYDVWLAQ"
     gene            complement(68467..69228)
                     /locus_tag="PL162_00620"
     CDS             complement(68467..69228)
                     /locus_tag="PL162_00620"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HQF90564.1"
                     /translation="MEPLTWGTALFLVVAGFFASFVDSVVGGGGLISLPSLLFLGLPP
                     QLALGTNKLAGVISSFTSSLAFLRSGRVEMEIVRWFFPLSLGGSVLGVLTVRRVPPDF
                     LRPLVLVMLVLVALYTVCRKEWGQHATYAGPTPRVWVWGGLASLGLGFYDGFFGPGTG
                     SFLLFVFLMLGFDFVRGAGNARVLNFASNIAAIVTFALLDSVQYTAGLVMGIGMVAGA
                     LAGTRLALTKGVAYVRPLFLSVTTVLIGKQIWDLF"
     gene            69653..70405
                     /locus_tag="PL162_00625"
     CDS             69653..70405
                     /locus_tag="PL162_00625"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQF90565.1"
                     /translation="MELNLWHVGAIVAVGLAQGFLNIVAGGGAFLVLPMLTFLGMDLA
                     MANGTNRIAILLQSVSGATSFRRDKILSFRSALPLALVATLGATAGAFTAVKVDKATL
                     NIVVAGLISVMAVLLLGKPEMWEGRKDRRWSRWMTYALFAGIGFYGGFIQAGAGLFIS
                     WGLAAAAGVDLVEGNAIKSVIIGCYTAVSLAIFFANGMVDPPVGLVLAVGSVVGAWIG
                     AKFTVAKGNRWIRRILVVVVIVSAAKMIGDVW"
     gene            70618..72156
                     /locus_tag="PL162_00630"
     CDS             70618..72156
                     /locus_tag="PL162_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HQF90566.1"
                     /translation="MVCTAHPLAAQAGLEILKKGGNAVDAAVATAAALTVVEPTSNGI
                     GSDAFALVWKDGRLRGLNASGPCPGSLSLDTARREGWSAVPPLGWAPVTVPGAPAAWA
                     ELTSQMGRLSLRENLAPAVAYAREGHAVSVTVGRCMAESFQRYARESGELFAPWFETF
                     APAGRPLRIGERWASRDHAETLERIGDSDARDFYRGELAERMVAHARRTGGFLTLDDL
                     GAFAPEWVEPLHGAYKGFDVWEMPPNGQGLVALLALSILREFSFPDHHDPLTVHRQIE
                     AIKMAFADAHRYVADPRCAPVPVEELLSEEYTRSRRALLRSEAGDFSAGNPLPGGTVY
                     LCTADGEGTMVSYIQSNYMGFGSGVVVPGTGIALNNRGHCFSLTPGHPNVLAPGKRPY
                     NTIIPGFLTRHGEALGPFGVMGGFMQPQGHVQVVMNTVDFGMNPQEALDAPRWQWMGG
                     MKVSVEPGFPSALAQALARKGHAVTCERESLSFGRGEIIWRTDQGTLAGATEPRADGG
                     VAPW"
     gene            72685..75183
                     /locus_tag="PL162_00635"
     CDS             72685..75183
                     /locus_tag="PL162_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQF90567.1"
                     /translation="MRNTTGTTWRVLGGLLGAVWVLWGSAGGVWADNAFLSTPRVWVN
                     GAWHKYGPSLGGINAAVTKTVFDMTISYHDTLSADSVPPGDPKRTRALEVEEVIKFWS
                     DALYEQSNGQHMLGTVYIYQGGKMGNKASVLWVATSHPSGLPGGYGKPLGNINFGDVF
                     GTNVFVGATSTATSQANGGYTLGHETGHFVYAMYDEYSANYAPAHFPRSRDVSPSSPA
                     IMNDQWGAMRDNQVRWLNHSTAGNYKASTDTAQKRVYGKSCWEVMLQDPSLDGPNARP
                     VAVWPQRVQYAFGNPPASPDWYRTAVTPGTHLAAAQANLRIVWLSGGMVYQIIIDRSG
                     SMVPDPDPVNPGPSPLDNAKGAARDLVESMAKETAVGVVQFDDGVAQVYPITFIPSDD
                     VGARAVKDATKAAIDGISKGGFTAIYDAASTALANIKTYQGTVSGDLTGACYLLTDGD
                     DNSSSKSSAQVIAEYQAAKIPLVTVGYGSGGAGNPVLSSLADETGGQYFTSPTTQSEL
                     QQVFFAAQGSYSDVVNLGNVKKTIAAGVADQALFRVDSTMGSVKFFLNWNGTSADLTI
                     TVRNPGGIAVPVTPQISQTGGASDYSAEVKAPTPGLWTVHVQNTAATPREVSFSAVGQ
                     PVEGTGFALTVGLANGGSTVTPPDPIRLFATVSKGGVPLAHATVVATITAPSSSTPTL
                     LTLRDDGTGGDAVAGDGTYSALLASYVAGTYKIDVTASNPSGTARESFWSYSPSRPGP
                     GDEGLVFNPDGALAGEDFQRSTSFQVKASGGVTPTAAPTPTGAPTVAPTVAPTVIPTA
                     GPGGGGGGGCATGMLAFPWAVVLLVPLLGWGRKP"
BASE COUNT        13075 a        22866 c        26031 g        13427 t
ORIGIN      
        1 tggcggagaa aatcgagccg ggacgacacg gctcccaggc cgtcggcggg caccgccttg
       61 atccggaagg ggaaaagccc cgggctgtcc aggagccctt tcagcggagc cagggccgtg
      121 ccccgggcgt agagggaggc ctccaccagc caggcgatgg gccggggatc ctccacggcg
      181 aggggagtcc cggcggcgta gacccccttg acgccgctct ctttgagcac gccccagtcc
      241 acgtacgacc gcagcacacg gcgcgccgcc cgggaaacgg tatcccgctc gccgtattgc
      301 tcccggatcc ggcgctggac atgggcggcg gcggcggagc cctggagcct cagaagacgg
      361 ccggtctgga tggccacctc cccccagaac ggatagacgg ccatgatcat gccccagtgg
      421 aaagccacgt gatcgcatcg aggagcgcat gagaggagtt ccagcccctc atcgcgccac
      481 gactccaggc ccttcggcac cgtcagccac gtcctgagga gaatagtgat gatcttctcc
      541 cgattgcccc gctcggcctg accaccgacg gaaaccttgt cctgcaacaa atcctgcaag
      601 gtgttcatca ccgccgcctt gtcattccct gccaaaatca ggttgaccgt ctgctcaaac
      661 cattccagac gcacccgctg actgaatccg atctggtcgg tctgcctcgt catgggaccc
      721 ttctctctgc ctgggcgctg cccccgggca tgtcaattcg gatgaaaggc acgatgagtt
      781 cctcgacgga aatgccgccg tggctcacga tgcgctcccc ctcccggaca aaggcccgcc
      841 gaggaggagc cagaagggga aagtacttct ccgggagacc gacgggatcc cacgccatgg
      901 cgtcggggaa gcgtttgtgc acgccttccc gaagggcccc atcgggatag atgcggaccc
      961 gttcgccgcg cagatccgcc acggcccctt cggagggacg accgcacccc tccgcctcaa
     1021 tgttgccgtg gtcggacgtc aggtagacca ggaagccccg ctccaggagc agatcgagga
     1081 gggcccccac atagggctgc cgggcccact gccccacctg gttgtgcatc ccggaggtgc
     1141 ccagtgccat gccgtgcatg atcttatcga ccttatccac cgccagcccg acgactttgg
     1201 ccttgggatg ggaaaggtcg tcggcaacct tttccaggtc ctcatccttc aggaccttcc
     1261 gatagacgac ctcctggggc gtgaggccct gatccctcca gaactgggcc cagagcgcgg
     1321 gttctctgtc cgtggtctgg atgctcttgg aaaaaaagag ggggatcttg ccggcgaagg
     1381 tggcctggcg cgagacggag gtgacggaag gaatccaggc aaaaaccgcc tcctcccgga
     1441 atcgcagccc ggggtgacgc gaggcgaggg cttcgcggac caccagccat tgatccaggg
     1501 cgaggccatc caccacgatg agggcgatct tggccttgcg gtcctcgccc atctgccggg
     1561 caaggaaccg gggaagatgg tgcagcatca ccggaggctc cggagggaga ttcaccagcc
     1621 cggcgtaccg cttgacgagc caggcggcga aggcgccatc cacccgggct tgcaagctcc
     1681 tgaagcgctc gcctccaggg gcgggcaccg ggccgtccgg agggttcgcc aaaaggatgg
     1741 tctccgccca cccccgggca aagtggaacc aatccgggtg cttcgcctcc tcggcgggga
     1801 tggagccctc caggcggtcc aggagcttgt ccaggcgacg gagccggtct tcggtgggat
     1861 cggtgcgaac gccgatgccc acccacgttc tggaaagcac gtccccgtgg tcgtggggca
     1921 cggcgtgcag gatcccctcg acgaagagat tgtcgatgta gacccggatg tcctcgtggt
     1981 cgaaggacag ttccgccggt cccgcaacgg ccagcggaga aagggccctc ggtgcgaaca
     2041 cgcgggacag ccctttggcc gcttcgtggt ccagaaaccg gggccagcgt tcctgaagaa
     2101 aggcgaaaaa cgcctcccgg tctgaaacaa ggatctccag gggccaatca ttgaaggcgg
     2161 cgttctggcg gagcagttgg ataaaccgcc cgtcgagatc cgggccgaag ttctgtcggc
     2221 ggtagtgacg gcgcagcaac gtgtgcagca ggtccgtagg gtgtcggacc agctcggggg
     2281 cgatgtcgaa aacgtgccgg aggatgaaat ccctggtggc gttgtccccc aagggagcgg
     2341 ggggatgggg caccaaggcc ccatacaagg cgtccagatc cccccgatcc agggctgcca
     2401 ccaccgaata actcagggaa ggaaagatgt cgccgaggtg gaaggagagt ttccgcccgg
     2461 cccggagaag atcgtggggc aacccgccgg gatcgctggc ctgggaacgc agcaccacca
     2521 ccaggtccgt gtcctcgccc tggtcccacc gggagcggaa cttcgattcg taggcataac
     2581 ggaacgcgat atggtcttca aacggcatca gctcaaagcc ccgcccccgg atgccttcaa
     2641 gcatcccctc ttcgaggaga agtccgtccg gatcggccgc cagggtaagc cgggagacct
     2701 ttggggtgaa ctctttcagg atttgatcgc gccagctcct catggccatg ctcctcctcc
     2761 caagttccac gccgccgcac tctgcggggc ggcgatgtgg tcctactccc cccccgagcg
     2821 ggtgagcgcc tggtcgtacc acatgaggag cttggaatct tcctgaagga cattctcggg
     2881 aaccttgcgg gccacggcga tgatggtggc gtagtcccgc tcctgccagg ccttcttgaa
     2941 tccggcccgg atggcctcca gacgaaacac cttgagcttt ctcgtgccgg aggccttgta
     3001 ctcctcaaac tccttgagca gggaccgttc ccggagcttt tccagatcgc cggccttgtt
     3061 cgggtcgggg gggtaccagc ggtccttgcc cttggcccgc agggcttcat cgtccttggg
     3121 caggtttcgg aggtccttga agttcgacga gagatagctg tggatttggt tcggcaccgc
     3181 ccccttgccg tcgtagcgga ggaagttctg ctccagcagt tccgaaagct ccagggtctt
     3241 ctcatacttt tgccagccgc caagctcttt gatgaacaaa ggatggatgt cctgaaatgt
     3301 ctgaggcttg tctttgagga gttggcgaag ccaggcgatg gcggaggatt cgtcggagac
     3361 aaagagtgac atctgctcca tgccatcggt ggtgatcctc tttttgtcgt attcggtggc
     3421 ctgctccggg aggaagtaca tgccgtcgcg gtgcggaaaa cgatgggcca agccttcctg
     3481 aaactcggcg ctgtccagcg gcaccgggta ccctttgcga acatagtagg ccaccatctg
     3541 gtcgaaaaga atgcgtgaat cgcgctccgg aacaaattgc aagacggctc cgtgtttttt
     3601 tacgacgggc agatagctca ggtgcgtgcg gacaaagtcc caaacgcctt cttccgtgtc
     3661 tgcctcaagg tgaaagcgtt cttcaaggcc cccgttgggc ttgtaggcgg agatgaccag
     3721 gtcttgcttg acggccaccg ccgtggtaac cgctttgaag ctgccctgtt gcttgtccag
     3781 cacggagaca ttggccacca caaaaccagc ctgccgcatg gccgtttgta atccattcca
     3841 caccgccgcc cgggtattgg agaatgccac cgttatccag cggccgggct tgagcacacg
     3901 aaaatattct tcaaagcacc cggccatcag tttgcgatag tcgtccaccg acttctgtgc
     3961 cgcgcggttt tccttggcgc gatccacgat ggcgtccagc ttcgcctgag tcatcacgcc
     4021 gagccacgct tccacgagga aattcaactc ggcatagggc agattctcgc caaagggggg
     4081 atcggtgaag atgtagtcga tgctgttctc cggcagaagc agccgggctg ccgtccccgt
     4141 gccgatggcc gcccctccgg cacctgtttt gaaatgctga aatgccttgg ccagccgatc
     4201 ccgcttgccc tccaggatgt accaggggct gcattcggcg tgctgagagg cgacgtagta
     4261 gactccggtc aattggcgat taacttgaga acctccaggg cggccttgct gaattggctg
     4321 atagcgatta agcacggaaa gcccccatat cgcctgctcc accatgaaga gcagaaaggc
     4381 ccgcaggcgg ggctccggat gggcccgggc ccttgcccac agagccccca tggcctgggc
     4441 ggcgcggggc aagaacatgt gatgggtgtg ggtgatgccc ttgtcgcgca tacggggagc
     4501 ttcccacatg tctgcgaagg ggaattcgac cagtggcacc gcagcgggca aaggcaggac
     4561 ttcaattttt tccagcagtg tcagatcggt cgcatcgggt tttttctcgt gccggatctt
     4621 gcccacacgg taggaaatca gactgggcac acgcttgggc gcttgcagaa tcttccccgt
     4681 ggcggggtcc agcttgctga tgcggatccg gtcaagtttt ttcttcgaca tctcggcgcc
     4741 gcagtgggga cagggaaagg tgtcccggac gcgctgggaa tcctcatcca gggcctcatc
     4801 caaaaaattc acttcccccg aacattccgg gcaccggaac acctcgctcc acaccgtgta
     4861 ctcgatgcgc cctttggtct tgccatcgct gtgccaggtt tcgtacatcc agcccagatc
     4921 ttgctccaca gcgtccaaga gctcttttcc cgccgaggca aaggcttcca cgtcgaaggg
     4981 catgttgtag ttggccgcga tgaacgtggc ggcgggggag aggtcgttca gcaccgcccg
     5041 gcgcgcccca atcttggaga atggcctcca gacaggtttc ccattctcgt cagtctcttg
     5101 ccgggaaata gtcccatccc tgtccacccg gtagcccagg gactccacca cacccttgtc
     5161 gccgcacatc tgggccgcca ctcccgtcat ccccgagccg caaaatccgt ccagaaccac
     5221 gtcgcccggc tcggtgtagt gcaggatgga cggcacgatg gcccggtggg gcaccttggt
     5281 gtggtaggag tgggccttgt agagggggtc cgtcttcccc tcgctcacat ccaccgcgaa
     5341 gggctcccgg cgataggcca ccgttccgcc ggcggagggg ttctccgagg gggacgtgtt
     5401 ctcgtctccc gggggggctg gcggagtctc cggtgtttcc tctgcggcga gaaaatctcc
     5461 cagccacgga ttcgggcagg cggtgtaata gggtgggtcg gagagcgcca ggatggcctc
     5521 gtcctcaccg agggggaacc cttcgatctt tcggaactcc gggtcttgga gcttctcgcg
     5581 cagaaggccg aggaagtgct cccgccgcgc gttgtcgtcg gggaaggttt tgcccaggca
     5641 tgtcacagag cccaagtcgc cagtggacgg agacgtttca aagagattgg cctggttgtt
     5701 ttggtctttt ttcatggcat ctcccccgtg tcggaaattt tgctgcctgc gctatgcacg
     5761 cgtacatagc gcacgataac acgcgtgcat agattatgac tctcgtgtaa ggagctccac
     5821 ttcctcaaaa gtttgcagga ggggtttgcg gaagaattcg ttgacgcgga catccgtgcc
     5881 ttccaggatc tgaaaggcga cttcgtggga ggggatgtgt tgacgaatag tttcttcgtg
     5941 ccccaggagg agcaatccca caacggtcgg ccggcgagtc ccgtcaacag tggtgacaag
     6001 ccccaacgca ccatccagtt cttcatcggc cagcggcagc aatgtcatat cgccaccgta
     6061 gcgttagggt ttctccctga tctaggatcg tcttgattcc gtgtccccgg atgtccatgg
     6121 gcagtgccta ctccaacacg attcttacct tgccgagctc tttgccccgg gtgagcccgt
     6181 ccaggtactc ctcgaagcgc ttgcgcagct cccccggggt ggcgggagat ccccccgcaa
     6241 gcagggcgtt ccgcaggtcg tcggccttca ccgcaacctt ggtgagcccg gagagggctt
     6301 ccacgagggc ggagatgaag ccctgatcca ggggctccgg cagggtccgc gtcttgatga
     6361 agccgtccac ccgttcgtgg ggttcgggct tgaggaggtg caggttgtcc ttggtggtgg
     6421 ggtcttcaag gttggcgagc agggtctggg tccagttctc gaccagattt tcgagctcgc
     6481 cgtccagggc gtcgagcatg gttgccgcag gggccgtggg cggttccgcg cccggtttga
     6541 attcgcagtg cggacagacc ggcgaggcct ccagttcccg ctcggtgagt gcggagcaac
     6601 tcctcaggcc agccaagcgg ttccggaagt ccaagagctg ctggcggggc atgaggtcga
     6661 tggtggcgag atcctgcagg ttcttgagcc gtccgtcgcc caggagctgg gctttgcgct
     6721 ggtcctcgtt cacgccgaga cgcgctttgg cgtgcagaga aaggtaggcc cgcacgtacg
     6781 cccttttgag gtcgtcaagt ttgcgctggg tttccgtgcg aaagttgggg gcgctccgct
     6841 tggccggatc gacgatctgg gcgaggacct cgtgccgcgc cgtcttcatg gtggtgatcc
     6901 actcgtggtc cgtgggcagg atcgtctcgg cgatggagag gtacgatgcg gcgggccgga
     6961 gatctcccac cagatcctcc agggacttga cctcggccag ggtgtggagg ccgctccgct
     7021 gggcggcgac ctcttcggcg ccgtagcgga agttcttgag ctttccggtg gtgctgtagg
     7081 gtttcaggga ttcgaggaat tccttggttt tcctcagcgt ttccgaaagt tttttcattt
     7141 cctcgtcgtt cagcaggctg cggttccaga agaacacgtc gctttccagg cgacgctgcg
     7201 cgcggagcac ctcttcgatg gcgttggtga cggccttctg gagctcctga acgggctcgt
     7261 ctttcccctg ggtgaccagt tgggccatgc ccgacgcgag tccgagcagt tcgaaaaggt
     7321 ttttgagcgc cgggaggttc cagtccttgg ggtgtttgat gtgcttgaaa ttgaccagct
     7381 cgtccacggc ggtccccgcc aggagagcca tccccgtggc gtcgaagtcc tgccccggaa
     7441 tggaaaggac cacctcgccg gcgtagacca gcgccgccag caccaccacg gcccatgcgg
     7501 gttcgaggcg aaagcccccg ggggcgaggt attccacgcc aaggatgtcg tggatgagct
     7561 cggagcggtt gaccacctgg ccggagccct tcttcccgag gacgtcgagg atgtacttgg
     7621 cgtacgggga ctggccgggg tcgaggcggt tgccgtcgag gagctccagg gcatcgagca
     7681 ccgccgtggc ctgcttggtg cgattctgtc cggccatggc gcgcagggcg tcctgggcgg
     7741 cctgcgcccg gttcgctccg gtgatgagca cggaaaattt cggatatgcc ggggcctggt
     7801 cgtgaaacgc cgggccgagg cagatccccg ccacggtgtt caccaggtcg cggaagttga
     7861 tgtgctcctg ggggccgatg ccggaaagct ctcggaaagg cttgcccttg gcccactcgg
     7921 tgagcgattt ggtgcgcccc tggtgcgtga tgtcgaaggc cttggtcatg tgctgccgca
     7981 gccactctgc gaggtcttga agggcttttc ccctcttgtc cttatagacg gcgttggcgt
     8041 gtcccgaaga tgtggaggcc agatccagcg ccgccgcata gctgcgcagg gcggtgcgga
     8101 acgtttcggc ggggtcgggg ggctggccgg ggtcgaacaa ccggggcgat ccccccgagg
     8161 gctggggctt cttcggggat tccaggcgca gccggaaaaa cacttcgtcg cctttctgct
     8221 cgtccttgaa gtgcggcgga tcaaagggct ggatgaagta gaggtagaaa tcccgcgacg
     8281 gcaccgccgt ggagcgttcg ttgggggcgc cgaagaagag gtacccctgg cgcatggcct
     8341 tgtgctcctg ccattcgagc tcgtggggcc agatcttgta gcccgtgacg taggtctggt
     8401 ccgcgcattc caagacctgc tggagagctt cgtaataggc gcggtcgagc tgggaggcat
     8461 cgaggctctc ggcccgcttt tcgatgaggg cgtcgaagtc gtcggtcttt ttgaggtcca
     8521 gatagtgctg gcggttgtcc cggttggacg aaataaactg tccgctcacg gttttgtgga
     8581 tttcccgcag gacaatctcc acctgggaga gcaggtcgtc ggcgggggtt ccgcccagat
     8641 cctcgatctc cggttggtag aggcacagtc cgtcccgcag ctcctccgcc gtggccccca
     8701 ggggggtgga gatgtcgcct gtggtgagcc gatggacgga gagcgcgtgg acgatccgca
     8761 gggccatggg tttgtagggc ggtcgggtaa aggcctgctg gatgcgggac tccagcaccg
     8821 cactgcaatc catgaccgcc ctgacgtccg gaacggagcg gaaggacgaa ttctcgcaca
     8881 gggtggccca gtagccgtcg taggcgatga ctccggggtg gtcttggggc acgttctggc
     8941 tgaggagttt cttcatggcc agggacaggg ttttgaggac ctcgcgcttt tccaccgcag
     9001 tgacccgctc gaaggtgtcg atgtaatcgg ggtgcacggg gaagaggcgc acgaattcgt
     9061 ccaggcgctc gttcatgcgg ccgtagaact tggcgaaggg ggtcaggtac tcccggattt
     9121 tctcctgctg gtgggcggtc ttcttgagca gacgctcggc caccacgaac ttgacgtccc
     9181 tgcgggcgat gaggatttga tcgaagcggt ccttcacccg gcggaggctt tcggacacga
     9241 aggaaaagcg ggagctgtcg aaaatggctt cctgaactcc ggcgatgaag cggaaccgca
     9301 ggtccttgca gacctcgccg atctcgcgca ggaagttgag atcgaggatg atttcctgat
     9361 ccttgcgggt gcgcaggtag tcgaggagtt cgtccaccac caggagcagg ccgtggtcgg
     9421 ggtacttctg gtggaaggcg gtcatcatct cttcaaagga acgcttgttg ttggaaacct
     9481 tatctgcggg ggggaaggaa taagacaccc ccatggcggc caggtgctcc tcaagctcgg
     9541 ccacaaggat gtcgcgcagg gacatgatgg tggcgccgat ctcggtgcgg actaccttga
     9601 aacgcccgcc gatcttgccc gcggcctccg ccacgctcgt gtcgttcaga tgcggtgaga
     9661 gatcgcccct ttcagccaga gcggagatca cggacagaag gtgggacttt cccgtgccgt
     9721 agttgcctac caccagcaat cccttgtggt ccaagggttt gtcgaactgg agctggggca
     9781 cgatgatatc ggtccaccga tcggccatct ccgaggagat gacataggtc tggacaagct
     9841 gctgggcctc ggcggccttg tcggcgttcc gaagcgccac cacgctttct atgggttcaa
     9901 actggatcaa atctccgtag gtcatcgtcc tcgccctcct gggtgtttgt tcctgttctt
     9961 catgcgatgg gggaaatctg gggtgggggc agtgtccggt ggttgtggcg atcccctgta
    10021 ggggcgagcg tattttgctc gcccctacgc tccacgtcct cgggagtcac caccaggaga
    10081 tcgtgtgcgg gatatcgttt gtattcgggg tggtccggcg tggcgtaggc cagagaggac
    10141 gatgggggct gggccgagga tgcccccggt ttcggcgcag tctggatttc tccgttccat
    10201 gcggcgacca ccgttttgtt ccgggagagt ccctgcagga ggcgcaacgg atcctgccgg
    10261 agcgaggggt caaagaggat ctcggtgttg tccagaagga ccaccgggac cccggaggcg
    10321 tccacaatct ccgcgagaag gcgggatgcc tgcaacgccc gctggcgctc ggtgaggtcg
    10381 agcagcgcgc gggacagttc gagattgacg ttgatcagag gggcccccgt gcgcctctgg
    10441 acctcccgga gggcggcggt ttttcccgca ccggccgggg ccaccaccag cacgaggcgg
    10501 tggtacagct cggcggcctg gttgattttt tccatgattt tgtcggcgag gggttctccc
    10561 atgagcatct cctcctgaac cgggttggtg gtgccgcgcc ttgcaaggcc gggggccacg
    10621 ggtttttcct gaaaggtcgg cgcggggcgg tgggccggga gggaatctgc cgttcctctc
    10681 tccgagggcg ccggggggcg atggggggag cgtggcggcg ggggaggctt tccccgaggg
    10741 atggcacgtc ccgggggttc tcggagcgca ccgctcatgg tccaaggcaa aaggcaacgc
    10801 cgtatcttca tctaatttag tgttattgct cggcgctgtc aagcgttagg gaaggggcaa
    10861 gggtgacttc atggacggat gtggcgccgg cgggggggag tctgcggatt gtggcggtaa
    10921 ccggtgatgg gggaaatctg ggggtggggg cagtgtccgg tggttgtggt ggtcccctgt
    10981 aggggcgaaa aatttttcgc ccctacgcgg gtccgggagg tttgccccga cgatgggttt
    11041 gaagtcttct cccctacgcc gggccagtgc cgatcccggg ggtttcgccc ctacgatgtg
    11101 ttcgggagtt tggttgagtt cgaggatgtc gcggggaaag tttttccctt tgttgttttg
    11161 tttgacatcg taagggaatg tgtcggtttt tttgttgttt ttctgatcag cgtggcataa
    11221 ccgataacct gccatttttc tgtaggggcg aaaaatcttt cgcccctacg ccggaccaca
    11281 ggttctcgtt tcattcaaat ttttttcccg acaccaaccc cacaactttt ggtcccttcc
    11341 gcgtggctcc gggcatcaca tgggcaaacc tcttccatgt aaattccctc cgggcaccgc
    11401 tcctgcgggt tcggtgccgt gcggaaaaat atcccctgca cgacctacgc ccaccttgtg
    11461 aaattttagg atggggctcc gggggtttcc agcaccgccc ctcggggggc gctggagacg
    11521 gcggcgcggt agcgctccag cagggagctc tctgtgggcc tcggaggagg aaggccctcg
    11581 cgccgtcggc gggccagttc gtcgtcggcc acgaggaggt cgaggcgccg ggcggggata
    11641 tcgaccctga tccggtcctg ctcccgcacg agggccaggg gacccccggc ggcggcttcg
    11701 ggggccacat ggccgatggc ggccccccgg gtggcgccgg agaaccttcc gtcggtgacc
    11761 aacgccacgg aggcgtccag gccgcgcccc gccagcgccg ccgtgggggt gagcatctcc
    11821 cgcatgccgg ggcccccccg ggggccttcg tagcggatga ccaccacgtc tcccgggcgg
    11881 atttctcccc ccagaatggc cgccgaggcg gcttcttcgc cgtcgaagat ccgggcgggg
    11941 ccttcgtggc ggcgcatttc cggggccacg gcggcctgtt tgaccacggc gccttcgggg
    12001 gcgaggtttc cccggaggac cgcgatgccc ccttcggcgt gcacgggatt cgccaggggg
    12061 cggatgatgc gggggtcgat ctcccccgtg tggtgcaggt tttcttctac ggagaggccg
    12121 gtgacggtga gggccctctg gtccaggagg cccccttccg ccaggcgggc catgacgccc
    12181 cgcacgccgc cggcggcatg gaggtcttcg aggtggtggg gccccccggg gctcatggcg
    12241 cagaggtggg ggcaccggcg gccgatgtcg tcgaagcggt ccaggggcag ggtgatcccc
    12301 gccgccgccg cgatggcggg aagatggagg gcggagttcg tggagccccc cagggccaga
    12361 tccaccgcca cggcgttgtc gaaggctccg ggggtgagga tgcgccgggg gcgaaggtcc
    12421 cgctccagga gggtcatgat ctgtcttccc gcgtcttttg ccaggcgctc ccgggcggcg
    12481 tgcacggcgg ggatggtgcc gttgccgggc agggccagcc ccagggcttc catgaggcag
    12541 ttcatggtgt tggcggtgaa catgccggcg caggagccgc atccggggca ggcgtcttcg
    12601 gcgagggctt cgagctcgtg ggggtccatg cgtccggcgg catgggcccc cacggcttca
    12661 aagacggagg agaggtccac ggggcgtccc cggtggcgtc cggcgagcat ggggcccccg
    12721 ctcaggatga gggcggggag gtcgagccgg gcggcggcca tggccatgcc cggaacgatt
    12781 ttgtcgcagt tggtgacgag gaccagggcg tccagggcgt gggcccgggc catgatttcc
    12841 acggagtcgg cgatgaggtc ccggctcggc agggagtagc gcatgcctgc gtggttcatg
    12901 gcgaggccgt cgcagacgcc gatgacgggg aactccaggg ggagtcctcc ggcggcgtag
    12961 actccggcct tgacggcctc ggtcaggcgt cccaggtggg tgtggccggg gatgatggcg
    13021 ttggcggcgt tgacgacgcc cacccagggg cgttcgattt cccaatcggt gtatcctgcg
    13081 gctttgagga gggcacggtg gggggcgcga ggggcccccc gtttggcggt gtcgcttggc
    13141 atgatgtgct cctttcgtgg ggtggttttt gggaaagagc gggagaaaag ggcaagagag
    13201 caagagagca agagagccgg ctggaaaatg cgcgctccca gcaaggagtt cccggcaagg
    13261 cgcgcccggc ggggtggggt tcggtggggc ggtattggca aggtgctccc ggggaggggc
    13321 gttcggctgt ggcggggcga gcatgttttt cgcccctacg gcgtgatgaa tgggttgtgt
    13381 gggggcgaaa aatttttcgc ccctacggtg gggtcaccgt gttgaggggg ctccggcttc
    13441 gatgaagttc tccagggctt ctccggcggg aaccatggga aagaccatct cttcccgggg
    13501 aaggcgcacg tccaccaggg aggggccccg ggcggcgaag gccccatcca gggcggggcc
    13561 cagctcctgg ggggtctgga cggtccaccc ccgggcgccg aaggcctcgg ccagggcggc
    13621 gaagcggcac ccttctcccg tttccgtggc ggaaaagcgc tccttccaga agagtttctg
    13681 ccactggcgt accatgccca gggaaccatt gttgaagacg atgatgcgcg ccggaacgcc
    13741 ctcccggacc agggtgtcca gttcctggac gttcatgagg aggctcccgt ctccggccac
    13801 acacaccagg ggacggtggg gggccccttg atgggccccc agggcggcgg ggaggccgaa
    13861 tcccatggtg ccgagccccc ccgaggagat aaactttcgg gggtggtcca ggggccagtg
    13921 gagggcggtc cacatctggt gctggcccac ttcggtggtc accagggcgt cttcccccgc
    13981 ccgggcccgc acggcgtcca gcacgtccca gggggcgtag ccgtcggtgc ggcggggatc
    14041 ccgcaggggg tgcttctgcc tccagctttc cagggtggcg ccccactccc ggcggtgttc
    14101 ttccggagcg gcgggggcgg cggcgagggc ttcgaggatc ttccgggctt ctcccacgag
    14161 gcccagatgg acggggacgt ttttgccgag ctccgccgga tcgaggtcgg cgtggatcac
    14221 ccgggcccgg ggggcgaagc gcgccacctg tcccgtgctc cggtcgctga agcgcgtccc
    14281 caccgccacc accaggtcgg cttcggtgag ggcccggttg gccaccgggg tgccgtgcat
    14341 gcccagaagg ccaagagcca ggggagagct ttcgggaaag gcccccttgc ccatgagggt
    14401 ggtggccacg gggacgccgt gccgtgtcgc cgcgcggaag agggggtccc aggcctctcc
    14461 ccggatgacg cctcccccca cgaggaagac ggggcgttcg gcccgccgaa ggaggtccgc
    14521 cgcttcgtcg aggcgggaga ggtcttccca tgccccgggg cggtatccgg gaaaggtcac
    14581 ctcggcgggg tagaaaaagg gccccgaggc ccggagcacg tccacgggga tggtgaccag
    14641 cacggggccg gggcggccgg aggcggcgag gtagaaggcc ccccggaggg cccgggggag
    14701 ctcggccagg tgctggacaa agaagctgtg tttcaccacc gagagggtca tgccgaagat
    14761 gtccgcctcc tgaaaggcgt cgctccccag gagggggcgg gccacctgcc ccgcgatgac
    14821 caccaggggg acggaatcca tggcggcggt ggccatgccg gtgatgacgt tggtggcgcc
    14881 ggggcccgag gtggcgatgc atacgccggg gcgccccgtc acccgggcgt agccgtcggc
    14941 ggcgtggcac gccgcctgct cgtgccgcac gaggatatgg cggaaggggg cgtccatgag
    15001 ggcgtcgtac agggggatga tggggcctcc ggggatgccg aagaccaccg gcaccccttc
    15061 ggcttcaagg gcttttacca ccatttgagc tccggtgcag gtcatggccc ttcctccaga
    15121 cacgccacca cggtttcgcc cacggcccgg gtggaagcgt ggcctccgag ctcccggggg
    15181 aggttttccc ggggagattg ttccagagcg cgctccacgg cccggtgtac cgccgaggcg
    15241 gcctccggct cgttcaggct ttccagaagg agcccggcgg agaggatcgc cgccaggggg
    15301 ttggcgatgc cccgcccggc gatgtccgga gcggagccgt ggacgggctc gaacatgccg
    15361 aggccggagc ccagattggc ccccgccgcc aggccgaggc cccccgccag ggcccccagg
    15421 aggtcgctga cgatgtctcc gaacatgttg gggcagagca gggtgcggta cttttcgggg
    15481 gagcgcacca ggtggtagca cagggcgtcc acgttcacca cgtcgagggg catgtggtgc
    15541 cgccgggctt cgtcccgggc cacttcttcc caaaagccgt agagctgggg catggcgttg
    15601 gccttggagg ccaggatcaa acgcgattct tgccgcttct gggccgcttc gcaggcgaac
    15661 gcggtgatgc ggcgcacgcc cccttcggtg gccagcccca cctggagggc tccggagccg
    15721 gggggggaga gctcccagga gccccggctc cgcagggtat aggtttggcg ctccaaggcg
    15781 gtgtccagat ggagttttcc ctcctccagc cgcccgccga ggcccacgta gaagtcttcg
    15841 gtgttttccc gcaccaccag ggcgtccagg cgggttccct ccgccagggt cacgggaagg
    15901 ggaacgcggg gatagctccg ggcggggcgg aggttgatgt acatgtcaaa atggtttcgc
    15961 aggcggagca ggatgccccg ctccaggatg cccgggggca ccgaagggtc tccgatggcc
    16021 cccaggagaa gggcttcgca cgtggccaac tcccccaggg cgatgtcggg gaagatctcc
    16081 cccgaggccc ggtagtgcgc cgccccgaag gggagatcca gccagtccag agcaaagccg
    16141 taccgcgctc cggcggcctc cagcacccgg cgggcctccg ccgtgacttc gatcccgatg
    16201 ccgtccccgg ggaggcaggc gaggcggtgg gtccgctttt cgtgggtttt tctagagttc
    16261 atgaccgcgc ctcctggtgt tttttctggg ccaaccggcg gcgaatgtag ggaacgatgc
    16321 cgccggatgc cacgagctcc tgaagaaagg gggggaaggg ggccccggtg taccgcgctc
    16381 cccgggtgag gttttccacc gtgccctggg ccaggtcgat gcggagccgg tctcccgggg
    16441 cggtgtgccg ggctccttcg gggcagatga ggatggggag ccccacgttc agggcgttcc
    16501 gaaagaaaat ccgggcaaag gacgccgcca ccacgcagga gacccccgct cctttgagcg
    16561 ccaggggggc gtgctcccgg ctggaaccgc agccgaagtt ttctcccccc accaccacgt
    16621 ctccgggggt cacccgggag gcgaagtggg gatccaggtc ttccaggcaa tgggggccca
    16681 ggagcttggg gtcgctcgtc accagatagc gggcggggat gatgacatcg gtgtccacgt
    16741 cgtcgccgta acaccaggcg ctcccgtccc aacggccccc gggagcgggg gtcttctcct
    16801 cacgcaacgt cgtcccacca ggggtctcga agggagaagc cgaaggggtc gcggaagacg
    16861 atgtcctggg gttcttcccg gggggtggcg cgttcttcaa aggaaagatc tcgaaagggt
    16921 tcgccgaagg ggccgcagaa aagccccccc agaggaacgc gtttttgctg gggcgtcgga
    16981 gagacggaaa ggggagagat actccgacca catcccacgt gggcaaaggg acaggatcct
    17041 gcgccggaag ggttgaacgg atgctgcatg gtcacgacct cctttaaagt agggcccggg
    17101 ggcccgcaag ttttctgtgt ttcgtccacg acgtgctgca aaaagttctt gacggtgggg
    17161 caaaagagga atgattttga ttcacacctc ccgggggtcg gtgatgcgcc cggtaagggc
    17221 ggtggcggcg gccaccaaag gccccgccag atagacctcg ctctcggggt gccccatgcg
    17281 ccccgtgaag ttgcggttgc tcgtggcgag gcaccgctcc cccgccgcga ggattcccag
    17341 atggcccccc aggcaggggc cgcaggaggg ggtggagacc accgcccccg cttcgacgaa
    17401 ggtggcgatg tacccccggc ggagcgcttc tttgaagact tcgtacgagg cggggatgac
    17461 gacgcaacgc acgctgtccg ccacccgtcg gcctttcaga agccccgccg cgagctccag
    17521 atcccggagg cggccgttgg tgcaggatcc gatgaagacc tggtccacgg tgacgtcggc
    17581 gaaggttttg gccgggcgca ccgccgccgg agaggggggg gccgccacga ggggttccag
    17641 gcttccggcg tcgatctcca ccacggcttc gtaggaggcc ccttcgtcgg ggtagatggg
    17701 gtggaagggg cgggccttgc gggcttcggc gtaggcgagg atcggggcgt ccggcacgaa
    17761 gacccccgcc ttggcgccgc actccaccgc catgttggcc acggtgaatc ggtcgtccag
    17821 ggtgagatgg gcaaaggctt ccccggagaa ctccagggcc ttgtagcgcg ccccctggac
    17881 gccgaggcgt tccagaagcg ccagcaccac atccttgccc cccacccagg gggaggggcg
    17941 cccctccacg gtcacccgca gggtgggggg cacgagaaac cacgtttccc ccagggccca
    18001 ggcggcggcg agatcggtgg agcccagccc cgtggcgaag gcccccaggg ctccgccggt
    18061 gcaggtgtgg ctgtccgccc ccaccaccac gtcgccgggg aggatgaggc cctgttccgg
    18121 cagaaaggcg tgttccaccc ccacccgccc cacttcccag tagcgcagcc cctgctcccg
    18181 ggcaaagtcc cgggcttcct tggcctgttg ggccgccgcc atgtctttgt tcggggtgaa
    18241 atggtccggc agaatggcgc atcttccggg atcgaaaacc ccttgcgccc ccatctcccg
    18301 aaaggcccgg atcgccggag gggcggtgat gtcgttggcg aaggcgaaat ccacccgcac
    18361 ggcgcagatt tctccttccc gcacgggggt ctgggagcgg gacgccagaa tggcgctagg
    18421 caacgtgtgt ttcacagagc gtcaccccct tggctgccgc gagttcatag aggcggttta
    18481 ccgccgacac gtaggccttg atgcttgatg ccaccacgtc ggtgctcgcc ccccgtcccc
    18541 gggccatcat gtcccccagg cggagcacca gggtggcctc gcccactgcg tccgaggcct
    18601 cgctggtggc ggtgacgttg tagtgcacca gttcgggagc aaggcccagg atgcgccgca
    18661 gggccgcgta ggctgcatcc acggggccgt tgcccgccgc cgcgtcggcg taggtggcgt
    18721 ccccttcccg gagggtcacc gaggccatgg cccgctccgt gcccgcgtgc acgtcgtagg
    18781 aatccagggt gtagcggcgc tggggggcca catcgagagt ttcgcccacc gccagggcct
    18841 ccagatcgcc gtcggagacg ctttccttgc ggtccgccag ttccttgaat ttcccgaagg
    18901 cttcgtccat ctgcgcttcg gagagggaat atcccagaga ttccacccgc tggcggaagg
    18961 cgtggcgtcc ggagtgcttg cccagcacga gcttggtgcc cgcagctccc acgtcttcgg
    19021 gggtcatgac ttcgtaggtg ccccgaaagc tcatgacgcc gtgctggtgg atccccgatt
    19081 cgtgggcgaa ggcgttggct cccaccacgg ccttgttcgg cggcacgagg acccccgtga
    19141 ggcgcgacac caggtgactc gcggcgcaga gccggggggt gtccacccgg gtccccaggg
    19201 cgaaacggtc ccggcgggtc cgcagggcca tgaccacttc ttccagggag gcgttccccg
    19261 cccgctctcc caggccgttc acggtgcact ccacctggcg ggcccccatc tgcaccgccg
    19321 ccagggagtt ggccaccgcc agccccaggt cgtcgtggca gtgaacggac cagaccacct
    19381 ccggccccgc ccccacgtcg tccaccaggc gccgcaccag ggccccgaac tctccgggat
    19441 cggcatagcc caccgtgtcc ggcacgttga gggtggtggc accgcacgac acggccacgc
    19501 ggaagacctc ggcgagaaag gcggggtccg accggctcgc gtcttcggcg gagaactcca
    19561 cgtcgtccac cagctcccgg gcgtgggaca cggcccggcg gacttcttcc agcacccgct
    19621 cccgggacat gcggagcttg tgggtcatgt ggatgtcgct ggtggcgatg aaggtgtgga
    19681 tgcgggggag ggcggcgtgg cggagggcct ttcccgcggc ttcgatgtcc ccccggtggg
    19741 cccgggccag gcccgccacg gtggaggtgc gcacttccgc cgcgatggcc gccacggcgt
    19801 ccagatcccc cggagaggcg gcgggaaagc ccgcctcgat gacgttcacc cggagcgccg
    19861 ccagctggcg ggcgatctgg agcttttcct cccgggagag ggagaccccc gcggcctgtt
    19921 cgccgtcccg gagggtggtg tcaaaaattc gtaccatgcc gtcgtccatg atttccaccc
    19981 cttcctcaga cgtccggagc ccctttgggc tccatccagg gcatcatgcg gcggagctcg
    20041 gcccccacct gttcgatggg gtgctggcgc tcccgggtca tccaggcctt catgcggggg
    20101 cgtcccgcct ggttttccag gatccactcc cgggcgaagg tgccgttttg cacccgctcc
    20161 agcagctctt tcatggtggc ccgcacccgg gcgtccacca ccgcgggacc cgccacgcaa
    20221 tccccgtatt tggcggtgtc gctcacggag tagcgcatcc accccaaacc cccttcaaac
    20281 atcatgtcca cgatgagttt cagttcgttg aggcactcaa aataggcgat ctcgggctgg
    20341 tatcccgctg ccgtgagggt gtcgaatccc gccttcacga gctctgtgac gcctccgcag
    20401 agcaccgcct gctctccgaa gaggtcggtc tcggtttctt cggcgaaggt ggtttccagc
    20461 atccctgccc gaccgcttcc cagggcggcg gcgtaggcca gggcgaggtc tcgggcctgt
    20521 cccgaagcgt cccggtacac cgcgaagagg gcgggaacgc ctttgccttc ggcgtacatg
    20581 cggcgcacga ggtgccccgg gctcttgggg gccaccatga aaacgtcggt gtctgctccg
    20641 ggttcaatct ggccgaagtg cacggaaaat ccgtgggcga aggccagggc ggcgccggga
    20701 cggaggtttt cccggacgtc gcgccgccac acgtcggcct ggagatggtc ggggatcaaa
    20761 aacatcacca cgtctccccg ggcggcggca tctgctccgg acaacacctc gaagccatcg
    20821 ctccgggccc ggtcccggga gcggctcgtc tcggggagtc ccaccaccac ggacacgccg
    20881 ctctcccgca ggttttgggc atgggcgtgc ccctgacttc cgtatcccag caccgccacc
    20941 gtcttgcctc gcagtacatc ccgccgcacg tctccgtcgt aatacacctt ggccattctt
    21001 caacaccgct cccttcgtct gttcaagccg tgatcttttc aagcccttcg tgctccggcc
    21061 cttgcgggcc gggaagatct cttctacgcc gtcagcgccg cggcgctggg gaaggggtct
    21121 tccagttctt cgtcctggcc gaatcccacc cgctccaggg ccaccgcccc gctggtgacg
    21181 gcttccagca cgccaaaggg gcgcatggcc tccagaaagg cccgaccctt tccctggtct
    21241 ccggtgagct cgaagaccac ggcgtcccgc cccatgtcca ccacgcggca ccggaagatt
    21301 cctgcggtct ggagcacggc ggcccgctgt tccagggggg cgcggacctt caccagaaag
    21361 agctggcgtt ccaccaggtt tcccgaggcg agatggcgca catccaccgt ttccaccagc
    21421 ttttccagct ggtgcaccat ctgccgcacc gcccccgctt ccgccttcac cacgagggtg
    21481 aaccgggaaa aattcggctg ctccgtggcc cccacgctga ggctgctcac gttgtacccc
    21541 cgccgggaga ccagacccgc caatcgggca aggacgccgg gacgatcttc cgccaaaacg
    21601 ctgatggtgt gccgcatgac cggaccctcc tgttttctct tcgtctcccg ctggccgtga
    21661 agcgggtaca aaaaaagagg ccgggatccc gatttcgggg tcccagcctc tcgatgcgcc
    21721 ctgcggtgga ttttcttgcc tacacgccac cgagcgctcc gggaggggac ccgcagcgaa
    21781 taatcagggc taccagcaga atcagaccca ggttgagcag cacatctgcg gccaatctgt
    21841 tcacttcatc ccctccttcg ctttcggatg caccccgccg ggggcggcgt gttggcgggg
    21901 attatggggc tatcgatgga tcgttgtcaa gggggcataa aaaagaaaga aaagacagtt
    21961 gatttataaa atttgtaatt accttcggag ctccgaagct tagggccacc ttttagcgcg
    22021 ggttttgggt acagccgccc cggagggcgg attttttccc cggttcggga ggggtgaagc
    22081 ccctggggcg ggcctacccc ttcttccctc tggccccagg gggagacgtc ccgggcggcg
    22141 gggcccgggc gcgcccttgt gccagagggg tgcggcgccg gagagggggc gtcgtcagag
    22201 gtggggtgcc ccggggcctc cgaaaggggc ggagaggtgt cagggggagg gttttatgct
    22261 gagccggggg gagaagggtt gattttattt gatctttcgc cggtcaaacg cacggccacc
    22321 gtcctaggct tgcccctctc tccctggcgg gggaagaggg gggccttggt ttcggggatc
    22381 gacatttcct tcggaggcac gccccggggc ctttaaggac acgttctatc ttgagatcga
    22441 tcttttcggg gaaggggcgc gggagaaact ccgattagct agccccctcc cgacgtcgcc
    22501 ccccggagct ccacccggtt tccccggggt tccagggtcc accggatccg ggtgagggcg
    22561 ctcaggaacg cttcgtcgtg gctcaccacg aggagggcgc aggggcatgt ggccagggcc
    22621 ccttccagga gctccacggc gggaaggtcc agatggttcg tgggttcgtc cagcaccagc
    22681 agatggggtt cttccaacac ccccaaggcc agcagaattt tccggagctc tcccggagag
    22741 gcgcttcctc cggcgaagat ccccgcgggg tcggacccca ggcagcgcac caccgtgccc
    22801 accaggcccc gacgcgccgg aggaagggcc cggagggtgc ggagggtccg agcttcgtcc
    22861 tcggcggaga gttcctgggg aagaaagagg atgcggtggg gaggaagctc ccagcgatcc
    22921 tgaagatacc ccaggagggt agattttccc gacccgttgg ggcccaccag ggccacccgg
    22981 tccgaaggcc ccatggaaag acagggaatg tcaagagttc gatgctctcc cagaggcaaa
    23041 gaaaacccag aaatattaag aattcgattg cgggaggagc gagctccgga gagccagaag
    23101 tgcgccgggg ggcttttgcg gatttccagg gccttcagcg tgtcccggct tcgctggagc
    23161 cgtccttcga tttgattttg gaaccgtccc gcctggccgt cttttcccga cacccggacc
    23221 aggttgatca tggcccggcc gtcggagtcc cccttggcca gggagcgttt ggtgcaccgg
    23281 cggtccgcgc tccgggccgc ctccttgcgg cgcttttctt cctcttccag gcgcttcact
    23341 tctcggcgct ggagatcccg ggccgtttcc gcagcctgcc gttcctcttg ccggacccgg
    23401 gacgcggtgc ggtagcttcc cggcaccagg actccccggg ggggatccag gaagaggcat
    23461 tgggtaacca gcgtgtccag cagctcccgg tcgtggctca ccaggagtcc cacgccccgg
    23521 aaggtcccca gggcgcgccc cagcagggcc cgggcagcgg catccacgtg gtttgtgggt
    23581 tcatcaagga ggaggagctc gggatcttgc cagagggcca cggcgatctg ggcccgcttc
    23641 cgctcccccg ggctcagggt gtgccatcgg tggggccacg agggatccag ccccaggatt
    23701 ccccggagac gggctccccg ggggtcccgg gattgaagga ggggttcgag ttcctcgggg
    23761 cagagttcca cccgctgggg acaccaggcc cgccgccggg gaccctggac cgctccggac
    23821 cggggctcca gctctcccag agccaggcgc aggagggtgg tttttcccgc gccgttggct
    23881 cccaccactc cggtccatcc cggagcaaag gccaccgtga ggttatggaa gacctccgga
    23941 gccgccgtgt cgtatcgaaa gctcacatgg gaaaatgtca gcacagtgca agacctcccg
    24001 gttttcgtac gggggggtgt ggtccgcttc gcccgatctc cccgaaagga gaaaagtttt
    24061 ttacgaagaa agggttcctc tcatcgccct cgggtttttc ccgggcacgg ggtgggtttt
    24121 ctgggggcgc cgggcgtcag gaagaaaaga gcccaggaaa aaaggcgagt tcttcggact
    24181 ttccgcagcg ggatggttcc cacgagagga atccggggag agggcacgtc aacgccgctc
    24241 cctcaggcga agcggaaggc gaaaacaccc catggggcgc cctggtcgtg gtgagggcgc
    24301 gaagaaattg ccaatgccat ggaagaggtt ttcgcactag cagtgacgca tggggttctc
    24361 ctttgtgggg atggaaacgc tcaagcccgg ggggcgtggg ccatgttgaa acggagggta
    24421 catcttcggc cccgggggtg tcaagggggc tcggtcgggg ttggggacgc tgaaaagaag
    24481 gaaagtgctt ttcccctcga caaagggcaa aaaaggtcaa attttcgtca cgcgtttttg
    24541 ggccaaaaaa tctccgaaaa tcccccctgg ggggaggtgt ggcgggaaaa acttttgcca
    24601 taccggaggg agaggccccc gggggatttc tttgaagggg gccaaggggg tatgatagtg
    24661 gggcaaagtg tgcccaatcc ccagggttgc gtggaaaacg ttttctccgc aggaggggcg
    24721 gaggagatct tttccgggaa cttgaggggg gcggggcaaa aaaccctgtg gaggtggtac
    24781 ggtcatgcgt ctgtcagcga acgtttggcg aagatttttt cttgcggcgc cgttttttct
    24841 ggtggtcctt ttcaccgccg catcctgtgg cagtggtggc ggtgggggga cctcctacga
    24901 aaagaccatg acccagatga cctcgtttat cgaggagcag atggagaaag accaggtcac
    24961 cggcctgggc atcgccctgg tggacgaaga tcgcacggtg tgggcccggg gttttggcta
    25021 tgcggacgag gcccagcaaa ttcccgccac caaagatacg ctttttgaga tcgggtcgaa
    25081 ctccaagacc atggtggccg ccctggtgat gcagctcgtg gaggcggggc gactgcacct
    25141 caacgatccc ctggaaaagc acatcccgaa cttcgccatg aaaaaacccc tgggatcctt
    25201 cccctccccg gggggagagg tcaccatccg gtacatgttg aatcaccatt cggggctgcc
    25261 gggagatctt tttaacgggg ccttcgctgt ggggaaaagc gatcctaagt ttaatgatta
    25321 tctagtggcc tatctggcgg gggagtacgg agattttccg ccgggatttt tgctggcgta
    25381 ctccaacaca gcgtattcgc tgctggccga cgtggtggcc gccgcttcgg ggcagagctt
    25441 tcaggagcgc agccaggcct ttttccggga tctgggcatg gaccacacca cctttttccg
    25501 ggaaagcgct gcggtgcccc cacatcagtc caaggggtac ctgatggggc aggagtacgg
    25561 ccccttctac tgcaatattc ctgcggcggg atcggtgctt tccagcgctt cggacatggc
    25621 gaagtacctg aaaatggtgc tggcgggagg cgtgggagaa cggaacaggg ttttgtccaa
    25681 ggaaacgctc tcgaccatgt tgacccggac caacggatcg gtggccctgg acggcgatgt
    25741 gagcgtgggg ctcggatggt ttctcagcga cgaccccctg acctatgcgg ggcgcctttg
    25801 ctggcataac ggtggcacgc tcctcatgat gagccacatg gaaattttgc gggatcacgg
    25861 gctgggggtg gtggtgatga ccaactccat caccggcgcc gcggtggccc atgatgcggc
    25921 gcacaaggcc ctgcaactgg cgctggaaga aaagaagggc cttcggccgc cgaaagctcc
    25981 tgatcccgat ccttctccgg tggtgacctg gaccgacgcc caactggacg ccgtggcggg
    26041 gatctacgtg acgacgacga cgggatacga tcgcctcgtc cgggaggacg gaggactccg
    26101 gtgggtcgag aacgcccacg gtgcggcggc ccgggatggc tcgggggatg tattggggga
    26161 gacggggcgg cgcctggtgc cccgggccaa cgggtggttt tcctcccccg attcccaggg
    26221 ggagcagttt gaatttgtgg agatggaggg gcggcgggtc atgatcagcc acacgtccac
    26281 cttcaaggga ttgttggcgg agcgctacga gccccctgcg gatttttccg acgcctggaa
    26341 ggcccggcgg gggatctacg acctggtgaa tctccgggaa gatgacgcct cccgggccct
    26401 tcctccggaa tatgggttca acagttttac ggcggagttc gatatccagg atgggctttt
    26461 ggtgctggaa ctcacccagg ggttgcgcaa gggcatgcgg tacgtgatcc atcccctttc
    26521 cgacacgttg ggataccttc gggggttggg gcgcaatctc ggcagctcgg tgcagatcat
    26581 ttccaccggt gcgggggagg agctccagct gctggggttg cgctaccgga agcgttaggg
    26641 aggggatccg gcgcggggtt ttgggccctg gggggacacg ccccaggggt ttccgggggg
    26701 cggcgctccc cccagggtgg ggaaacgttc tttgcggtga cacatttcgg gctccggggc
    26761 gcgccgtgcg ctccggagcc cgaaatgcgt ctgagggagt cttcgtcgtc gcttttcccc
    26821 cggagggggg gcggaagggt cacgctccct tttggggaga agggggctcc tcctggggtg
    26881 cgggagacgg gcggcgttgg ctccagagga aggcgtccgg ggtgtagagc cccagtccca
    26941 gccagatggc gccgaaggtc acccccgggg tgacgtcgaa gacctctccg tagacgagca
    27001 cccccagggc gaacgagagg gagggggaga cgtactgcag aaggcccacg gtgatcaggg
    27061 gaatgcgggc ggcgccaaag gcgaagagca ggaggggcac cgaggtgatg accccggcac
    27121 aggtcagcag ggcgtcccgg aagaggggcc cgtgccgaaa agcggttgcc cccggagggc
    27181 gagaaatccc agataggcaa gggcaggggc ggagagcagg agggtctcca cgaagaggcc
    27241 gggaatggag gccacggcaa gttttttccg caacagacca taggaaccga aggtcacggc
    27301 gagcaggagg gaaacccagg gcagggatcc acgatctccg ctcccttctt ggtcacagga
    27361 gccgacagaa aaatttcttg cgcaatgatc gcatccatgc acgtgttggc ttgtgcaagg
    27421 gtcagaccgt ccttggggta tttcaaagag agggtccaat ccttggaatc ggcacaagta
    27481 aaggtaagtt tcagcacttt tgccatgagt attcacctcc tccgttcaac tacatcgctt
    27541 tccctacgcc accaccagat ccttatcgtt cttgatcatc tcgacgaggc tatgggccag
    27601 aacggcaccg atggcgttac cgactgcata catcgcatta gccgtggccc ccgcatcgat
    27661 gccgctcagc gtcatgctct tgacgtcagg cattcccgac ggcttaacgc ccacctgcac
    27721 ccgcagggca aacgaggatc ccgccgaagt atacgttgcc attgtttttc ccctcccttc
    27781 tggaaaatct ccatcatgga tggatcgtat cgctttcatg aaggttttgc aatacgcata
    27841 tttcgctcat atggtgcgaa ggtgcgcgct cccggcgtcc cgcttcctcc ttcccccgct
    27901 taccggcgcg ggtggccgta gatataaaaa aggctcctcc ctggaaggga gaggccgtga
    27961 tgcctggatg cttgctatag atatcttttt gggttgaacc cccactcaat ggcgacgtca
    28021 agcggcactc cgaggtccga aagctccaca ggcttcccgc gttcaatgaa tgagcggatg
    28081 tgggcgatgg cctcctcagc agaaagataa ttctgcatgt agtcgatggg gaagggccga
    28141 tggaacgctt ttgcgtatac cttcatctcc cgatcaatgc caggatcagc tggctgtgct
    28201 tccatgtcac actcccctcc ctgggctaag aagtcctcaa acgcctggac aacgttgggg
    28261 aacaggtcgc gaaccacctc gatttttgtc gtaggccaca tcatgccccc accagccacg
    28321 gatcttcccc gtcgctgacg ctggaatcat tatatcactt gctgctccta tccttgacaa
    28381 gccgcactgc ggcaatcttg tcgcccttcg ccctcgaccc tctttcccca tgcccagaaa
    28441 aaagtttttt tgcccgtcag aacccaccta aggtgtcgtt gcctggtgct ataatcgtga
    28501 aaaatgattt tgcgggcgga gatttcgcag ggccattttt tctccggggg aaagggccgt
    28561 gccgacgcgc tccaagaggg ccgtccgggc gggcggccct ctctccgaga caacagcggt
    28621 tttccggaac accatatcgc gggaggttcc gcgccgcttg ggccttgatg aagcatcaaa
    28681 aaatgtggag ggttgaccat gatcgagagc caaatcgaag accatctgat cgaaaaactg
    28741 accgacctca aatacaccta ccgttcggac atccacgacc gcgcggcgct ggagcgcaat
    28801 ttccgggggc acttcgaggc cttgaaccgc gtgcacctca cggacagcga gttcggcaga
    28861 ctgctggagc gcatcatcac cccggacgtc ttcgccgccg cccggacctt gcgcgagcgg
    28921 aacaccttcg agcgcgatga cggcaccccc ctccactaca ccctggtgaa catcaaggac
    28981 tggtgcaaga acaccttcga ggtcgtccac caggtgcgca tcagcaccga ctacagccac
    29041 caccgctacg acgtgatcct cctgatcaac ggcgttcctg cggtgcagat cgagctgaag
    29101 accctgcaga tcagcccgcg caaggccatg cagcagatcg tggagtacaa gaacgacccc
    29161 ggcaacggct acggaaaaac gctgctctgc ttcatccagc tcttcatcgt cagcaaccgc
    29221 accgatacgt ggtacttcgc caacaacaac caccgccatt tcagcttcga cgccgacgag
    29281 cgctttctgc cgctctacca gttcgcggac ccgcgcaacg ccaagatcac ccacctggac
    29341 agttttgccg aggcctttct ggccaagtgc accctggggg agatgatcag ccgctacatg
    29401 gtgcttgtgg cgtgcgaaca gaagctcctg atgatgcgcc cctatcagat ctacgccgtc
    29461 aaggccatcg tggattctat ttcccaaaac tgcggcaacg gctacatctg gcacaccacc
    29521 ggcagcggga agaccctcac ctccttcaag gcgtcgaccc tcctcaagga caaccccgac
    29581 atcgacaaat gcctcttcgt ggtggaccgc aaggacctgg accggcagac ccgggaggag
    29641 ttcaacaaat ttcaggaagg ttgcgtcgaa gagaacaccc acaccgaaag cctggtgcgg
    29701 cgcctccttt ccgaggacta tgccgacaaa gtcatcgtca ccaccatcca gaagctcggc
    29761 ctcgccctgg acggcaacaa cagccgacgc aactacgcgg agcgcctgga acccttgcgc
    29821 tccaagcgca tggtcttcat cttcgacgag tgccaccggt cgcagttcgg cgacaaccac
    29881 aaggccatca aggcgttctt tccccgcgcc cagctgttcg ggttcaccgg cacccccatc
    29941 ttcgaggaaa acgccaccca tcagcagatc gaggggcagc aggccagcta ccgcaccacg
    30001 gtggacgtct tccggcagca actccacgcc tacaccatca cccacgccat cgaagaccga
    30061 aacgttctgc gcttccacgt ggactacttc aaaccggacg gaaaggcgcc ccgcggggcg
    30121 gacggatccc tcaccaagaa ggccatcgtg gaagcgattc tcgccaagca cgacgccgcc
    30181 accttcgggc gccggttcaa cgccctgctg gccaccgcgt ccatcaacga cgccatcgag
    30241 taccacgcgc tcttcaagac ggtgcaggcc gaaaaagccg cggcggacga gcgcttctcg
    30301 ccgctccacg tggcctgcgt cttctctccc cccgccgagg gcaacaagga cgtgcagcag
    30361 atccaggaag acctgcccca ggaaagaatc gacaacgagc aggagcccga cgcgaagaaa
    30421 gaggccctgg agggcatcat cgccagttac aacgcccgat acggcaccaa tcaccgcctg
    30481 ggggagttcg acgcctacta ccaggatgtg cagaagcgca tcaaggacca gcagtacccc
    30541 aacagcgact ttccccacaa agagaagatc gacgtcacca tcgtggtgga catgctcctc
    30601 acgggcttcg actccaagta cttgaacacc ctgtacgtgg acaagaacct caaacaccac
    30661 gggctgatcc aggccttttc ccgcaccaac cgggtgctca acgacaccaa gccctacggc
    30721 cacatcctcg actttcgcca gcagcaggac gccgtggaca ccgccatcgc cctcttctcc
    30781 ggcgagaccg ccaagcccgc caaggaaatc tggctggtgg agcaggcgtc ggtggtcatc
    30841 caaaagctcg acgacgccat ggagaagctg ggccaattca tgcgctccca ggggctggag
    30901 aacacccccg aggcggtgct ccacctcaaa ggggacgacg cccggacctc gtttatcaac
    30961 cagttcaagg aggtgcagcg cctcaagacc cagctggacc agtacaccga tctgacggac
    31021 gagcacaagc agaagatcga gcaggtactc cccaaggatc agttgcaggc cttcaagggg
    31081 gtctatctgg acaccgtgca gcgtatccgg gatgaacggg gcaagggagg cgtggacaag
    31141 aaacccgagg agggaggggg cggtcagctc gatcccgagc tcgtcctttt cgcctcggcg
    31201 gatatcgact acgattacat catggccctc atcgcgacgt attcccagca ggcccccgac
    31261 aagcagaaga tgacccggga gcagctcatc ggcctgatcc aggccgatgc caagttcctg
    31321 gacgaccggg acgacatcgc ggagtacatc ggcaccctgg aacagggcac ggggctggac
    31381 gaaacggccg tccgcgccgg gtaccagcgc ttcaaggagg agaagcacgc ccgggaggtg
    31441 gccgccgtgg ccgaaaggca cggcatcgaa cccgccgccc tgcgggcctt cgtggaggac
    31501 gtactgcgcc gcatggtctt cgacggcgac cggttgggcg acctcttcgc cctgcaggag
    31561 ctggggtgga aggcccgggc ccagaaagag caggacctca tggaggacct gctgccgttc
    31621 ttgaagaaac tcgcccaggg gcgggagata tcggggttgg gcgcctatga gcagtaaacc
    31681 ggaagaagcg ggcacgacgg cgaaggcggg gcggccgctg gtgcccacct tgcgctttcc
    31741 ggagttccgg gatgcggggg agtgggaaac cttgcctatg agaaaactgc tatcaagaga
    31801 tccagagtat ggagtgaatg caccgtcttc tccatattca gaagataaac taacatactt
    31861 aagaataact gacatagatg atgatggtaa ttttttaagc aactcgaagg tgtcagttga
    31921 tatcaatcct gttgatggga attttcttaa aaaaggtgat attgttctgg ctcgtacagg
    31981 cgcaagtgta ggaaaatcct atcgttacaa agacaaggat ggcccgctgg ttttcgctgg
    32041 ttttttgata cgtatcaggc cagaatcaac gaaagcggtc tctgcttttt tgtcgaattt
    32101 tttgacgaca caacagtatt ggagctgggt tggcgtaact tctacacgca gtggacagcc
    32161 tggtataaat ggaacggagt atgcatcatt gcccgttcca gttccccctg ctattgccga
    32221 acaacaaaaa atcgccgact gtctctcctc cgtcgatgac ctgatcgcct tggaggcccg
    32281 gaaactcgac gccctcaaga cccataaaaa gggcctgatg cagcagcttt tccccgccga
    32341 aggcgaaacc ctccccaaac tccgcttccc ggagttccgg gacgcggggg agtgggaaac
    32401 cttgcctatg agaaaactgc tatcaagaga tccagagtat ggagtgaatg caccgtcttc
    32461 tccatattca gaagataaac taacatactt aagaataact gacatagatg atgatggtaa
    32521 ttttttaagc aactcgaagg tgtcagttga tatcaatcct gttgatggga attttcttaa
    32581 aaaaggtgat attgttctgg ctcgtacagg cgcaagtgta ggaaaatcct atcgttacaa
    32641 agacaaggat ggcccgctgg ttttcgctgg ttttttgata cgtatcaggc cagaatcaac
    32701 gaaagcggtc tctgcttttt tgtcgaattt tttgacgaca caacagtatt ggagctgggt
    32761 tggcgtaact tctacacgca gtggacagcc tggtataaat ggaacggagt atgcatcatt
    32821 gcccgttcca gttccccctg ctattgccga acaacaaaaa atcgccgact gcctctcctc
    32881 cctcgacgac ctcatcgcct tggagaccca gaaactcgac gccctcaaga cccacaaaaa
    32941 gggcctgatg cagcagcttt tcccggtgtc ggacgaggtc cccgcatgac gcagctcatc
    33001 ctctacacca gcgaagacgg ccacgcccac gtgcagttgc gggcccatcg gggcacggtg
    33061 tggctcagcc agcggcaaat ggccgaattg ttcgatgtct cgccggacac tatcggcctg
    33121 catctgaaaa acatctacgc cgacggcgaa ctgcaacgcg cggcaactgc cgaggaatcc
    33181 tcggtagttc aaaccgaggg cgggcgcgag gtgcgccgct cggtcacgct ctacaacctt
    33241 gacgccattc tcgccgtggg gtaccgggtg cgctcgccgc gcggcgtgca gttccgccgc
    33301 tgggccagca ccgtgctggc ggagtatctg cgcaagggct tcgtcatgga cgacgagcgg
    33361 ctgaagaacc ccgacggtcg cccggactat ttcgacgaga tgttggcccg catccgcgat
    33421 atacgcgcct cggagaagcg cttctatcaa aaggtgcgcg atctctttgc cctggccgcc
    33481 gattacgaca agaccgatca ggccacccag acgtttttcg ccgccgtgca gaacatgctg
    33541 ctgtatgccg tcacccagtg caccgccgcc gaaatggtcc tccaccgcgc aaaccctgcg
    33601 gatccccatt tcggcctgag ggcctggacg ggccatcggg tgcgcaggca cgacattctg
    33661 gtggccaaaa actacctcac cgaggaggaa atcgacacgc tcaaccgtct ggtggtgatc
    33721 tttctcgaaa ccgccgaact gcgggccaag cggcagacgg tgaccaccat ggccttctgg
    33781 cgcgagaacg tggcgcagat catcgtggcc aacggctttc ccctgttggc gggggcggga
    33841 tcggtgagcc atgcccgcgt ggagtcgcac gtcacccccc tgtttctgga ctacgagcaa
    33901 cgccgcaaaa cccaagaagc ccaggaggcg gacgcccagg acgaggcgga gctgaaagcg
    33961 ctggaacaca gaataaagga tcgcaaggag gcggaatcat gatcgagcaa gacaaaagga
    34021 aactgggggc caccctctgg gccatcgccg accaactgcg cggcgccatg aatgcggacg
    34081 acttccggga ctacatgctc tccttccttt tcttgcgcta tctgtcggac aactacgacg
    34141 gggcggcgca gaaggagctc atgtccgatt atccgaagct gccgccaggc gaccgtcgcc
    34201 cccccttggc ggtgtggtac gaccaaaacc ccggcgatgt cgacgagttt gaaaaacaga
    34261 tgcgccgcaa ggcacactac gtgatcaagc cggagtacct ctgggggagc atcgccgaga
    34321 tggcccggat ccagaacggg gagttgctgc acaccctgga aaaggggttc tcctacatcg
    34381 agaacgaatc cttcgacagc accttcgccg gtctgttctc ggagatcaac ctgaattcgg
    34441 agaagctggg aaaggactac gtggcccgca acgccaagct ctgcaccatc atcggagaga
    34501 tcgccgaggg gctggcgcag ttttccgccg acagcgacac cctgggggac gcctacgagt
    34561 acctcatcgg ccagttcgcc gccggttcgg ggaagaaggc gggggagttc tacacgccgc
    34621 agcagatttc cagcatcctc tccggcatcg tcacgctgga cagccaggaa ccggccaccg
    34681 gtcggcgcaa aaacctcgaa agcgtcttcg acttcgcctg cggctccggt tccctgctgc
    34741 tcaacgtccg ccgccgcatg gggacaatgg gcatcggcaa gatctacggc caggagaaga
    34801 acatcaccac gtacaacctg gcgcgcatga acatgctgct ccacggagtg aaggattcgg
    34861 agttccagat ctaccacggc gacaccctca ccaacgactg ggatgcgctc cgggagacaa
    34921 atccggccaa gatgccgaag ttcgatgcgg tggtggccaa tccgccgttc agctaccgat
    34981 gggagcccac cgaggccctg ggcgacgaca tgcggttcaa gaaccacggt cttgcgccca
    35041 aatccgccgc ggacttcgcc tttctgctgc acggtttcca ctacctgaag ccggaagggg
    35101 tcatggccat cattctgccc cacggggtgc tcttccgggg cggcgccgag gaacgcatcc
    35161 gcacgaagct gctccaggac ggccacatcg acacggtcat cggcctcccc gccaaccttt
    35221 tcttctccac gagcattccg gtttgcgtcc tcgtgctaaa aaaatgcaag aagcccgacg
    35281 acgtgctttt catcaacgcc gccgaccact tcgagaaggg caagcgccag aatgtcctga
    35341 cgccggagca tatcgaaaaa attctcgaca cctaccaaca ccgccgggaa gaggaacgct
    35401 acgcccgccg ggtgtcgatg aaggagatcg aggagaacgg ctacaatctg aacatcacgc
    35461 gctacatcag cacccaggag gcggaggacc aaatcgatct tcgggcggtt aacgccgaac
    35521 tggtgggggt gacgcaaaag atcgaggagg cccgggagcg gcacaacgcc tttctccggg
    35581 agctggggtt gccccccgtg tagtagacgc gccgcaaggc cgtgtgcggc gtaggggcga
    35641 aaagagccgg cgggaaggta cgcgctcccg gcgtcccgct tcctccgccc tcggaggaag
    35701 ccagtccttg gagaggaaag gagaacatgc gatgaaagcg ctttctgtgc ggcaaccgta
    35761 tgccaccctc atcgccacag gtgaaaagac catcgaatgg cggtcctgga agaccccaca
    35821 ccgcggggcg ctgatgatct gctcgtcagc gtcctgggcg gaaggggagt gggatattcc
    35881 caaagaggac cgggatcaat ttccccgggg tgtcaccctc tgtatcgtgg atctggtgga
    35941 ctgtcgtccg ttcaagaaaa aggacctgga ggccgccttc atggaggaaa atccgggggg
    36001 gtatgcgtgg gttttggcga accccgtgcc catcgccccg aggcccatca aggggaaact
    36061 ccatctcttc gatgtcccgg caccttccga agcgggcgca gaggaacttt gttgaggagg
    36121 gggggctcgt cccccctcct ccttcgggtg atctccttcc gggcaatccc gaaagcccct
    36181 aagacgaaga cagggtccct tcccggcgga gtgcttccag ctgctctggg gtgatctccg
    36241 tgacgtagag aatcttctcg tccgactccc ccagggccag catcctccgg gccgtgtcga
    36301 tttccccttc catccggcct tccgcccagc cttctgcccg gcctttgctg aagccctttt
    36361 ttcccatcag gctgacgtcc atctccccct cctcctttcg gcgttccagt tcttcgtccc
    36421 actcttccca taccaggctg tctttgacgt tcataatccc ttcgatgaac cagagcagaa
    36481 acgccgagga ggttcctctt taccttgctt tacattgcct ttccagaact ttttctgtac
    36541 cctggggggg gatgtcctgt agtaaattgt tcggggggtg gagccatgcg gtgtttcatc
    36601 aagaggtgtt ccctcaggac ccagatcatg gggggcacgg cggttattgt ggtgggagct
    36661 ctggcggtgt ctttttgggg gagctatctc acggcccggg atcggctcct ggagagctcc
    36721 gtcacccagg gtcaaggggc cgtcaccagc ctggcccacg ccacggagaa caccttgaag
    36781 ctccttcgca cggcctgtgc cacgaacctc gacgtgagtg cggggaccgt ggcggggggg
    36841 cgcctcatgc ccagccggtt caccgtgacg atgggtaagt gggtgctcac ccccctttcc
    36901 ctttccacgg gcaacctcgt aaagatcaca ggaaacaacg cccttcccga ggaatgggca
    36961 gcccgcatgg gggggtgttt ctccctgttg cagcccgcct cggcggacgg cgccgtcgcg
    37021 ttggtgcgcg tttccacgtc gatcaaggat gcctcggggc agtccctgat cggtacgccc
    37081 ttggaagatg atagccccgc cacgccggcg ctggcccggg gggagagctt ttttggcccg
    37141 acggagatcc acggagagcc cttttttggg gggtatcgtt cggtgaaggg caagacgccg
    37201 gaggagcacg tcgtggtctt cgcgggggtt tcgttgacgc ctttgctgca attcctccgg
    37261 gagtcgcaca tcggcgaggg aagcgaggtc cgggtcttcg acgcccacgg gcgagagctg
    37321 ggaaagggag cctccccaga ggggtcttct tccctgtggg cggccctgga gccctggagg
    37381 gccaaaatag ctccggggga ggtggaatct ttctccgagg gcgagggcaa ggccgcgcgc
    37441 caggtgtttg tcttccggac ggattctccg gagtggaccg tggcggtgac cattccttcg
    37501 tcggtgatca ccgcccccct ggtggccatg cgagagcgga tgctcatctg gggcctcctc
    37561 tccctgggag ccggattggc cctggtgacc ttcctggtgg gacgccttct ggcccccctg
    37621 cggaacgtgg tggagacggc ccaccgcgtc gccatcgggg accttagggt tagcacctgt
    37681 gccgacgagg acgaagggag ccgcaacgag atctgcgtcg tcctttcggc cttcgcgcgt
    37741 ataggtacgg cctatcggga cttggtgagt cagatgcagg ggttgcaaga gctattccag
    37801 gaacggggag acgccatgga gcacatcaat ggcgaggtgg tggcatccct tcgagaggcc
    37861 ctgggggccg cccaggacat ggtgcgcacc gtggagagag tggtccaggg ggccgatggg
    37921 acaaaatccc gggtgacctc cgtcacccag gcctcccggg aggcttcgag cctcgtggcg
    37981 ggcctatccc gggagtcgga agaagtggcc cgggagacgt ccgcaaatgg ggaggcagtg
    38041 cgggcggtgg cggtgggcat ccgcgaggcg gggggggcca gcgtccgggt gcgggatgcc
    38101 ctgggggctt tggagagctc cgtggagagc atttcccggt ttgtggccac catccagggc
    38161 attgcggacc agacaaatct tttggcgctg aacgccgcca tcgaagccgc ccgggccgga
    38221 gaggcggggc ggggctttgc cgtggtggcc gaggaggtgc gcaagttggc ggaggactcc
    38281 aatggggctt ctcgccagat tcagagcgtc atcgaggaga ttagggcccg gacggacgag
    38341 acggtgaggg acacggagcg cgtcacggag ctcatcgccg ggacggagaa agactcccag
    38401 gagaccgccg aacgcattgg ggagatcgtg gcccacgtgt cggccattgc cgaaggtatt
    38461 cgatccatcg ccgccgtgac ccaaaaccag ctcgctggca acgaagcggt ggaaaacgcc
    38521 atggacatga tccatcgcca agcgggggag gggcagcgcg ccgcatcccg ggtggagcag
    38581 gtgtctcgcc aggtggcggg gcagatggat catctggaag agatccggcg tcaacagggc
    38641 gatgtcctgg aagatctggg ccgggccctg gcaggatacg tgctggacga tgcctcggag
    38701 gttcgggcgt tggaggaata agccctgggt cggggaaggg ggctcgtccg cacgggcgcg
    38761 cccccttccg gtcgtttgcc cccaggggtc cccgggagca ctggggtggc cgggatgggg
    38821 gagtccgttt tggggtccgg ggactaggcc tcccgtcgta gggaggaagc ctccggggag
    38881 gaacgtcggc tcaggaggaa tgaatccagg gtgtaaagcc ccagtcctat ccagatggcg
    38941 ccgaaggtca ctccccgggt gaagtcgaag gtctcgccgt aggctaatac gcccagggcg
    39001 aaggaaaggg acggggcgac atactggagg agtcccaccg taaccaaggg aatgcgggcg
    39061 gcgccgaagg cgaaaaggag caggggcacc gaggtgatga tgccggatcc ggacagaagg
    39121 gcgtctcgaa agagggaccc cgcgccgaag cactgctgcc cctgaagggc aagaaagccc
    39181 aggtacgcca gggcgggggc ggatagcagg agggtttcta cgaaaagtcc cgggatggaa
    39241 cccacgggga gcttttttct catcaatcca taggacccga aggtcacggc gaggaaaaga
    39301 gagacccagg gcaaggagcc gtggaggacg agctggtatc ccacccccag ggcggcgagg
    39361 gccacggcgg cccactgata ggggcgaagg cgttcatgaa aaaaaatcat gccaaagagg
    39421 acgttcacca ggggagtgat gaagtacccc aggcttgact ccaggatcct ccccgtgttc
    39481 acggcccaga tgtaggcgag ccagttgacg ccgatgaggg tgccgctgac ggcgagcccc
    39541 agggcgacgg agcgccggga gagggcgctc catacctcgc cccaccggcg ggtggccgag
    39601 agcatgatca ggagcaccag catggaccac aggatccggt ggcacaagat ctccaaaggg
    39661 ggaacctctt ccagggcctt ccagtagacg gggagggctc cccataggag gaaggccccc
    39721 agggtggccc aaaatcccgc ttgaaattcg gaggcgcgtt gcgtcgacac gatagatcaa
    39781 actcctttcg tccccttgga tctcctgggg gtgcttcgac cattcccggg gaggggcgct
    39841 tggggcgggg ccgtcgctcc ccaccagggg gatcatttct cggggaatct ttctggaaaa
    39901 ggtatagctt aaaggccccc cgatgtcccc ctccgaagga ggagctcttt gaaagccaaa
    39961 gccccatgga ggccctcgga tcgaaggatc ctcccgggag cagaggcttc tcatcgaggg
    40021 atgggccctg gggacctcgg cgatgcctcc cgaagaccgt ttctccggcc cctttgtgga
    40081 tctggttccc ggggcgccgg ggggagggac cgaaggacgc gaggggcttt tcttgccatt
    40141 cccttccccc ggtcctacaa tggagcgaag gggaaaaagg gacgtccagg ggcttgcgaa
    40201 cggcgaacaa tggtcctggg cccacggggc aggggccggg atgtctcggg aaagaggggg
    40261 ggaaagaggt ggcgaaggga agggggctgg gaccgtggat cctgggggtg gtgctgatcc
    40321 tcgctatccc ggcctggggg gtggacatcg gccgctggga tggccccgac ttggggttgg
    40381 gcctttcccg ggccttggtg cgctacctcc tgggatctcc cgatgcggtg gcgggaagga
    40441 cttgggtcta cgtcgtctct ggctctcggg agatcgacct gggattcgtg gatttttcct
    40501 ccggggacat ggtgtgcgtc gctgcccagc gctaccgccc cggaggggtc tcggcggtat
    40561 ccctggtgga gctcctccag cgccagggct tcgtctcggt ggtggcggac ggccatcgat
    40621 ggctcttgac gggagaggat ggctcttcgg ggcaggcggc gtttttcctg gtgctctccc
    40681 ctgatttggc ggagtctcct tctaggggcc ccctgctggc gacgctggac gagcgccctt
    40741 ggcgggccct gctggagggg gaggataggg ggcggacggc gtgggagcgc cgcttcgtag
    40801 ccatgatggc ttggcctcgc cccagagagg gggaaagccg tgacggggca ccctgaccgg
    40861 aggagaatgg aaagcctgcg gcgatggctt ttcctggtcg tggtgctgtg gggagccctg
    40921 atggggggcg ccgatggggc cctggccttg gctcgggtct ttcgcttcga ccacctggga
    40981 gtggggcagg gcctcccttc gggggtggtg caggccctgc tccaggaccg gacgggtttt
    41041 ctctggatcg gcacccgggg agggctctgt cgctacgacg gtcgggagac cctttccgtc
    41101 cccttggagc gcccgggaga gccccggcgg aggatgatcc gggccctggc ggaggacggc
    41161 gagggatatc tatgggtggg cacggggggc gacgggctgg tgcgccgcca tcaggcctcg
    41221 ggagagctca cagtctttgg ccggggactc ccgggggggc taggggggga caaggtcttt
    41281 tccctgctgg tggacgcccg gggggtgctt tgggtgggca cggaaggggg gttggcccgg
    41341 tacgaccggg tccacggagt ttttgaaccc tttccccgcc cgggaaagga cgccctggga
    41401 ttgccctcgg cgccggtgcg ggccctgtgc gaggacgtcc gggggcgctt ctgggtgggc
    41461 accgacgagg ggctggccct ggtggaccgg ggcacggggg aggtgcgcct cttccgccat
    41521 gggacggggg atccccggtc cttggggagg aacgacgtgc gcgccctctg tgccgatggg
    41581 gacggagccc tttgggtggg cacgggggga gcggggctgg atcgcttaga cccttcgggg
    41641 gagtttttta ttcatcatcg ccccgacccc caggccccgg gacgcctcgg tgggggcgcg
    41701 gtgcgggccc tgtgccgaga tggccggggc aacgtctggg tgggaacctg ggaagggggg
    41761 ctcttccggt tcgaccgaga aaaggggcgc ttcgagggtt ttcgccatac cccggggcgg
    41821 gcggggagcc tgggaaggaa cacggtgaac tgcctgttgg tggaccggtc cggcttgctg
    41881 tggatcgggt tggagggggg aggggcggcg tcgctgaacc tccgggcggc ggagaacttt
    41941 cccctgtaca cccacgaccc cggcgaccct tcggggttgg cttcttccat ggtcttggcc
    42001 ctgggccggg actcctcggg ggcggtgtgg gtggggaccg accgggggtt ggaccgcatc
    42061 gaccgcagtt ggaaatccgt gcgatccttt cgccccgacc cccagaggcc cgactccctg
    42121 gcgggggagt ccgtgcgggc ggtgctggcg gactcccggg gcttcctctg ggtgggaacg
    42181 gcgggaggag gacttcagcg cctttacctt ccggccctgg accggggggg attttttgcc
    42241 cccctgcggc gcgacccccc cttcagacgc ttttaccacg acccctcgcg ggagaactcc
    42301 ctggcggatg ccgatgtgac ggtgctgctg gaggaccgag aaggactcct ctgggtgggc
    42361 acggcggggg aggggttgga ttgcttcgat cctcgcaaag agacctttac ccaccatcgc
    42421 tacgaccccg atgccctttt ttccctgggg ggaaacaccg tgacctgcct tttggaggat
    42481 cgcttctctc acctatgggt ggggacggag ggcacggggc tcgcccggta cgaccgtagc
    42541 accgatcgct tcgtgcgcct gcgagaggac catggcaccc cttcggtggg gacccctact
    42601 tgccttctgg aagaccggcg ggggcggatc tgggtgggca ccgagggggg gttacggcgc
    42661 ctggaccgcg cccgaggggt gctggtggag gtccccgagg tgcgcccccc gggggaagac
    42721 gcagtgacgg ggatgctgga agacgacacg ggatccctct ggatcgccac ccgagggggg
    42781 ctttgccgct actggcccga gacgggacgc ttcgaggcct atggggcccg ggaggggctc
    42841 cagggggagg agtttaacta cggcgcctgc gtgcgaggat cggagggaga gctgatcttc
    42901 ggaggctccg gcggcctcaa cgccatccgg ccgggatggc tccggcgcaa tctcatgccc
    42961 cccggggtgg cgatcacggg agtggcggtg gaagggtctc ctctggatcc catgacgttg
    43021 gggtctttgg cccccgtgtc cctggggccc cgggatcgat cggtgacctt caccctggcg
    43081 gccctggatt tttcttcccc ggagcggaac cgtttttcgg taaagctgga gggcttcgag
    43141 acctcttggg tctccctggg atttcgcaac gtggtgactt acactaacct ggacccggga
    43201 tcttatgctc tctgggtcaa ggccgccaac gccgacggag tggagagccc cccggagcgc
    43261 ctgatccgcc tgacggtccc cgtgccgggg tggcgttcct ggtgggcgat ctcgctctac
    43321 gccctgggat ccctgggaat cctgggaggg tacggctcct tccgggtgcg ccgagaggag
    43381 cgggtcctcc gggagcagcg ggaacgggcc cgggccctgg aagaagaggt ggccctccgg
    43441 acccgggagc tccacgaaaa aaacgaggcc ctgcggcgga gcgcccagat gctccagatg
    43501 gaggtggagg agcgcctttt ggcccaggat gccctggtgg cggccaagga tgcggcggaa
    43561 agcgcctccc gggccaaggg ggtttttctg gccaacgtgg gacacgagct gaggaccccc
    43621 atgaacgcca tcttgggatt caccgatctc ctgctggaag gggagatctc tcccacccag
    43681 cgcccctggc tcgtcaccgt gcggagcgcg gcggggtccc tgcttcgcct cttgaatgac
    43741 ctgttggatc tctccaaggc cgaagcggac cgactggacc tggaagaagt gccctttgac
    43801 cctgcggcgg tactggagga ggtgcggagc cttctggagc cccgggcccg ggaaaagggg
    43861 ctggcctttt gcacggaagt ggacgcctcg gtgcccccaa tcctccgggg ggaccccttt
    43921 aggctgcgcc agatcctcac taacctggtg gataacagcg tcaagttcac cgccgcgggg
    43981 catgtggcgg tatttttgac ggcccagccg gagggggggc gggtgtggct ccggggtcga
    44041 gtggaagact cgggggtggg catttccccg gaccaggtgg gtaggctctt tgaacccttc
    44101 agccagggcg attcgtccac gagccgccgt tacggcgggt cggggctggg gctcgccatc
    44161 gtccggcgcc tggtggatct catggagggc tccctggagg tggcccctcg ggaggaggga
    44221 gggtcgattt tttccttctc tctctccttg ggccgagagg aaggtggggg cgaaggaaaa
    44281 acccataagg agggttctcc ggagctcgag ggggaggccc gggcccttcc tctggggctc
    44341 cgggtgctgg tggcggagga taaccccccc aataggcgct ttctggaggc ccttttgggg
    44401 cgttaccaat gggatctcac cttcgtggag gacggagagg aggcctggcg cctcctggcc
    44461 ccttcggggg aagggagcct gggttcgccc tttgacctgg cgctgctaga cgtgcagatg
    44521 ccgggcaagg acggccccga ggtggcccgg ctcctgcggg aggctccggg gggagatcgc
    44581 cttcctctgg tggccctttc cgccggggcg ggggaggggg agcggcgacg gtgcctggcc
    44641 tcgggcatgg acgcctttgc ccccaaaccc ctcacccggc gggccctgga gggagcggtg
    44701 cgggccgccc tggggcgccg gggcaagggc ggcgattttt tcgaggcccg agatcccgtt
    44761 gtcggaagct ggggggagct cttcgaagct tacgaggggc aagaagggga gctccgccgc
    44821 ctgctggagg acttccgggg ttccctgccg ggggtcttga gggccctgga agcgggagtg
    44881 ggaaaagaag accgagactt tctcctgcgc cggatccatc gcctcaaggg aagcctgggg
    44941 gccttttgga tggagcgctc cggggccctg gctcgggagg tggaaggggc cctgaagcgg
    45001 ggcgacgacg agggggcccg gcgagggctg gcccttttgg cccttcggag tgaggaagag
    45061 gggcgagccc tggggcgggc cttggaggat aggaggggaa cgcgatgacc caaggggatc
    45121 ggcgtcgact gctgctggtg gacgacgacg atgtcttccg caagaccacg gcgacccggc
    45181 ttcggcacct gggatacgag gtgcgggagg cctcggaggg agaagaggcc ctgggggcgg
    45241 tgtcggccga cctggacctg gtgctcctgg acttggggct tcccgaccga gacggggtgg
    45301 aggtcctggg ggtgctgagc tcggcttggc ccgagctccc cctcctggtg gtctccgggg
    45361 cggaagaggt gggcaccgcc gtggaggccc tgcggggcgg cgcctgggat tacctcctca
    45421 agcccttggg agatcgggaa agcctctccc acgccctgga gcgggccttc gagcgccggg
    45481 accttttgcg ggaaaatcga gctcaccggg aacacctgga ggaacaggtg gccgaccgga
    45541 cccggcgcct tcaggaggag ctcgccgaac ggagccgggt ggaagaagag ctgcgccgca
    45601 gccaagaaga ccttcgatcc ctggcgtcgg agctgtcgct ggcggaggaa cgggagcggc
    45661 ggcgggtggc cgtggatctg cacgatggcg tgggtcagac cttggcatta ctaaaaatga
    45721 aattttctca cctgcgtcgt acggcggggg aggccctgcc ctccaagccc ttcgacgaga
    45781 tcggggcgtt ggtggaggag gccattgcca gggtgagggc cctcaccctg gaggtgagct
    45841 cccccgtgct ctacgaagtg ggcttcgagg cggcggtgtc ggccctggcg gggaagatgt
    45901 tggacgaaca cggcattgcc tggaccttgc acgacgactg gcagcccaag cctctggccg
    45961 aggacgtgaa agtcctgctc ttccaagtgg tgcgagagct gctgcacaac gtggtgaagc
    46021 acgccgaggc ccggcgggtg accctttcgc ttcgccggga gggaagggag ctggtgtgca
    46081 cggtggccga cgacggcaaa gggggagtcc gggaagagga aaggccccag ggcggaggct
    46141 tcggcctttt tagcatccgg gagcgccttt tccacctggg ggcccgcttc gagcttcact
    46201 cctccccggg ggaagggacc acggggatcc ttcgggcgcc ccttctcatc ccccgagagg
    46261 cgtcgagaaa agaggaggcg aggcccccca tccgggtgct cctggcggac gaccacgacc
    46321 tcttccgccg gggggttagg gccctgctgg agccctacgc cgacctggat ctggtggggg
    46381 aggcggaaga cggagaggcg gccctggcgc tggcccggga gtgccacccc gacgtggtgc
    46441 tgctggatgt ctccatgggg cgccacgggg gcatcgaagc tgcccggcgc ttttccgaag
    46501 agtttcccaa aacggcaata ctaggacttt ccatgcacgc caaccccttc gtggtgcggg
    46561 aaatgctgga cgcaggggga gcgggatatc tgctgaaaga ctgcgatccg gaggagttgg
    46621 cccgggcgat ccgggccgtg gcggcggggg aggcggcctt cagcgccccc gtggcggcgg
    46681 cggtggccga ggacctggtg cgccagcgcc gccggggtca ggaagccctg tcggtccgag
    46741 aggaagaggt gctccggcgc ctcgccgagg gagaaagcat caaagagatc gccgccgccc
    46801 tgggcatctc cgccaagacg gtggaaacct accgggagcg catcggcgtc aagctggggc
    46861 ttcacaccgt ggtggatatg accaaatacg ccctgcgcaa ggggatagtg gcgctgtgaa
    46921 aggtgtggcg gcgccggaga gggaggagcg atggcctctt ttgtgttgaa aatttttgga
    46981 tgccagatga atgtctacga cggagaccgc ctgcgcaccg ccctggaggc cggagggtgg
    47041 cgagagggag aggaggaatc cgccgacgca gtgatatttc tcacctgtag catccgggac
    47101 aaggcggagc agaaggtgtg gagtgccctg gggcgctacg gcgaaagatg gcggggccag
    47161 gggcgcccca cggtggcggt gctggggtgc atggcccaga gggtgggagg agagctcttt
    47221 aggcgctttc cctgggtgcg ggtggtgtcc ggcccgaggc acctggggcg gattcccgag
    47281 gccctggaga gcgccttgag tgagaggagg cgccatctcc tgttggacga ggatccccgg
    47341 gccctggaag acctctgtgc ccccccctta aggcgagaaa acccgtacag ggcctttgtc
    47401 tccatcgccc acggctgcga taatttctgc gcctactgca tcgttcccca cgtgagagga
    47461 cggtttgcct cccgggatcc aggggccatc ctggacgaag tacgatgcct ggtggcctcg
    47521 ggggtcaagg acgtgaccct ggtggggcag aacgtgaaca gctacggcac cgaccgggtc
    47581 gacgggtgga cctttccccg gttgctccgg gaggcggggc gcgtgcgggg gctctggagg
    47641 cttcgcttcg ccacgaacca ccccaaagac ctcacggagg agctggggcg caccctggcg
    47701 gagggccctc ccctctgccc cgccctcaac ctgcccatcc aggcggggag cgatcgagtc
    47761 ctggccctga tgaatcgggg ctatacggtg gaggactacg cccttcgggt ggcgatgctc
    47821 cggcgctatc ttcccgatgt ggggctcacc agcgacctca tcgtggggtt tccgggggag
    47881 accgaagagg acttccggga gtccctggga gccctggagc gcttccgctt tgaccaggtc
    47941 catacggcgg cctactcgcc ccgtccgggc acccaggcgg cgtccatggc tccggcggtg
    48001 cccgaggaag agcgcttccg tcgcctggag gaggcgaact ccctccagcg tcgcatcgcc
    48061 acggagataa accgagaccg ggtggggcgc gtctatgaag tcctcctgga tgcccccgcc
    48121 ccccggggag agggttatct ccaggggagg actccccagg acaaggtggt gctggtgcgg
    48181 ggggaggtgg cgctgctggg caccatgggg gcggtgcgga tcacggcggg agaaccgtgg
    48241 ggcctccggg gagaatgggc taacgaaggg cccccggggg atgcgggggg cgtagaaggg
    48301 gaggaaaaag cgtgaaccta tcgccaaaaa cgcgccaaat gcttgttcta gcggggggga
    48361 tcctctgcct tctggtggcg tggatcctgg tgggtatctt tgcggggcgc tggaaggaga
    48421 cggctcccac gtcgatggcg gggcccgccc tttcgacgaa agaccccctc cccacctctc
    48481 cacccctcac ggagctcgtg gtgtatctca ccggggcggt gcgtcgtccc ggggtctttt
    48541 ccgtgccccc ggggagccgg ctggtggcgg tggtggagaa ggcgggaggg ctggcgcctg
    48601 aggccgatgc cgagaggatc aacctggccc agcccgtggc ggatggggcc catctccaca
    48661 tcccccggcg gggagatcgg gaacggctgg cggccctggc gcccccggga ggaggggcgc
    48721 cgacgtcgcc tgaggacccc gggagaccgg ttgacgtgaa ctccgcctcg gcggaagagc
    48781 tggagaagct ccccggcatc ggccccaaga ccgcccaggc tgtggtggaa gatcgggaga
    48841 gaaatggccc ttttcgctcc gtggacgacc tggggcgcgt caaaggcatt ggcccgaaga
    48901 aactcgaagc cctgcgcccc ctagtcactg cgggaccctg acccatgctg gggatcatgc
    48961 cctccatgcc gatctttctt gggtggtgcg ccgccttagg ggctctgggg ggtggagtgc
    49021 ctcccctggg ggcctggggg gtggggatgc tggtcacggc gggggcgatc tggagcaccg
    49081 ccctggcctt tccccgaaac ttctgggcgg tgggatcggc ggcgctcctt ctgggagccc
    49141 tgggcggtgc ggcggtggtg taccgggggg cctttccgga gcgtccggag gggaagatcg
    49201 aaggcgaggg gctggtgacg gcggagcgcc cctggggcgg tcgtcgggcc gtgctggtga
    49261 ccctgcccca gggaactttc ctggccttct cgtcgccggt gacggggctc cgggagggag
    49321 atcgggtggc ggtgaccggc accctagagg ccttcaggcg ctcccctgga gggtccttcg
    49381 acgccgagcg ctactggagg gcccgggggg ccaaggggga gcttcggggg gcctcctgga
    49441 ccctgctccc cccggagcgc tggtccctgg cggcggtgcg ctcctcgctg aggaaaaaga
    49501 tcctcctgac cctcccccct ctcacccggg ggtacctcct ggcggcccta gtgggggtct
    49561 ccgatccgga ccgggcggcg gttcatcgcc gggcggggac ggtgcacctg ctttcggtgt
    49621 cggggtttca cgtggggctg gtggggcttt ttggggccct gatcttccgg ggacgggggc
    49681 gatgggtctt atccagcgtt ctcatctggg tttacgtggc cctggcgggg gctcctccca
    49741 gcgcggtacg gtcggcggtg atggcccaag gggccctcct ggggtggacc ctggggcgcc
    49801 gagggggaat aacgaacgcc ctggcggtgg cgggaggggg tatgctgctc tggaatccct
    49861 ggtatttctg ggacgtgggg tggcgcctct ccgtcctggc caccctggcc ctggcccttt
    49921 tggcggaatc ccgccttccc caccgggtgg cggggatctt ggcgagcccc ggggcatgga
    49981 ttgccacggc gggggagtgc gcggcggttt ttgggccggt gcccctggcg gggttggcga
    50041 taaacctggt ggccctcccc gccttcggag tgctctttcc ggtggggatc ctggcggggt
    50101 tgccctccct actagagctc cccggggggt gggtgggggc cgacttcgcc gaggactgtt
    50161 ttgtcctctg ggaggggctt tccgacgcac tggtgaggct ctttcccggg agcgtgaccg
    50221 acgcctggat ccccacgggg atcggggcct tggctctggg gacgctgggg gggagggtgc
    50281 tgggccttcc cccgggacgc accctctttc tgggggcggc gacgatggcc ttttggggga
    50341 tgataaaact catctagcct ccggagcgga gggttcgggc cgatgtcagg gtgggggcaa
    50401 gaggggcagg gggtccacgg ccttgccatt ttttagcacg gtgaaggcca gttgagcggg
    50461 gccggagacc ggtgccggac ccacggcggc gaggcgatcg ccccgccgga ccaccgcccc
    50521 ttttttgcag tagaagacgc cgcagttgcc gtagaacgtg gcgatgccgt cggcgtgctg
    50581 gaggtacacc gcgttgccga agcccttcat ccacccggcg gccaacacga tgcccgcagc
    50641 ggcggcgcgg atgggggtgc cttcgggggc ctccagatga agagaagggg atccctcctg
    50701 accgaagcgc cggagcacgg cccctcgaag gggccaggcc agggcctcct ctcccggggt
    50761 ctgggaagcg acggaaaggt ggggagaagg gcgccccggc gccccggagg ggcggggtaa
    50821 aggggccacc cggagcacct ggcccggagt caggtgctgg gggtccggca gggcgttgag
    50881 ctcgaggaga aggtccaccg acacctgata tcgtcgggcc acggcaaaga gcgtgtcccc
    50941 cccttggacg gtgtgggtgg cgggccgggg agcggggaag tcctggggat ctcgggcgga
    51001 agggacgacc tccggtcggg gaggaagatc ggggctcgtg gcgccccggg gcaccaggag
    51061 gacctgcccc atggcgaggc gggagatgtc ttccaaccca ttgatccggg ccagggccgt
    51121 gacggaggta tgatatcgcc gggccacagc aaaaagtgtc tccccctcct ggacgacgtg
    51181 gcgttcaaag gcatccgtcg atagggggcg ggcgccgccg ctggcggcac cggaaggagc
    51241 ggctcttccc tcggcctttg ccagaaggat cgcccccagg gctacgaaga cgacgatggc
    51301 cttgcatcgc acgggatttt ctcccccttt cgtggctaaa gcatagcaca agggggaggc
    51361 gatgataagg ggtgggtcga agccgtggca taatggaggc cttgagggga cgccgaaggc
    51421 tcttcgggcg aggaccgaag aggacaacag taggaacggg ggacgattga tgcttttggt
    51481 gctcgacata ggcaatacca ccacggtgtt ggggctctac cgggaagata gcttggtgcg
    51541 atcctggcgc ctcacttcgg aaaaacgcac ggcggacgag ctctccgtct acctgcttac
    51601 ccttttggga acccttcggg tggatcccga agagatcacc ggggcggcga tggccagcgt
    51661 ggtgcccccc ctggatgccc cctggagcga ggcagtgaca gccctttgcg gagtgcaccc
    51721 cctggaggtc acctcgtcgc tcgatctggg gatgcccgtg tgcctgttga accccgggga
    51781 agtgggagcc gaccggctgg tgaacgccgt ggcggggatg gcggcgtacg gcaaacccct
    51841 cctggtggtg gacttcggca cggcggtgaa catcgacgtg gtggacgggg aagggcggta
    51901 ccggggaggg gcgctggctc cggggctggc cgtgagcatg gaggccctct tcggacgcac
    51961 cgccaaagtg cccaaggtgg ccctggcgcc cccgcccttt gccatcggac gctccaccca
    52021 ggaggcggtg cagtcgggga tcatcttcgg gtacgctggc ctcgtggacg ccctggtgcg
    52081 ccgcatgtgg aaggagctcg gcgccgaagc ccccgtggtg gccacggggg ggcaggcctc
    52141 tttggtggcg tctttttccg agaccatccg gagtgtggcc cccgacctca ccttggaggg
    52201 gctgcgtctg atccaccgca gacaccgagg atgaggcccg taaggagaaa taggggaggc
    52261 gaaagctggt gacgcagaag ggagtgaggc ccgggacgga aagatcgagg cgtaggggca
    52321 gcgaggcgga aaaatctcga gatggcacgg cagaacataa ggaggatgtc atgagaaaaa
    52381 accgaagaat cttggcgttg tgtgtggtgg ccctcttggt cctggttctg gggagcgtgg
    52441 cggaggccgc ccggttgacg gtgctccacg tgaacgacac ccatggccac gcgtggccct
    52501 ttgacctgta caacaacccg ggagtggggg gcttcgctcc cctggccacg ctgatccaag
    52561 agatccgcca ggaggtggag gcccaggggg ggcacgtgct cttgctccac gccggggatt
    52621 tcaacaccgg cgtccccgaa tcggaccaaa acgatgccat gccggacatc gtggccatga
    52681 acatgttgcg ttttgacgcg gtggggctgg gaaaccacga atttgacaag ccccgggagg
    52741 tgctggaccg acagggacgg ttcttggatt gccccctggt gaacgccaac atcctcaccg
    52801 gcgaagggaa acaggccttt gccccctaca tcctgaagga ctttggcgac gtgaaggtgg
    52861 gcctctacgg cctcaccacg gaagagacct ccaagctcga acccacctat ctgggggatt
    52921 ggaagttcga aaacgccgtc gagacctcca aacgcatcgt cggggagctt aaaaagagcg
    52981 ccgacgtggt catcgccctg gcgcacctgg gttgggccga ggcccccgca gcggggtaca
    53041 ccacgtcgaa gatgctggcg gaggcctccg tgcccgggct tgacgtcatt gtggacggcc
    53101 attcccacac gatctttgag cagccccccc tcatcggcac caccatggtg gtgtcggcgg
    53161 gggagtggac ccagtacctg gggcgccttg acctggtggt ggagcagggg accatcgtcg
    53221 gccggacgtg gcgcctcatc cccatcaacg ccaagacggt cacgggcaag gatgccgagg
    53281 gcaaagacgt cttgggcttc gtcgacaagg agatccccgc cgacaaagag gtggaggcgg
    53341 ccctgggata tttcctgaaa atcggtgccg aaaagctcgc cttggtggtg ggagagacca
    53401 aggtgctcct ggacggagag aggaacaacg tgagggcccg ggacaccaac ctatccaaca
    53461 tggtggccga cagcatgcgg tggaagacgg gggcggacat cgccctgatg aacggaggcg
    53521 gtgtgcgggc ctccatccaa cccggcccca tatcttatcg cgacatcctc acggtgatgc
    53581 ccttcggcaa caccctctac gtgatggagg tcacggggga agagatccaa aagctcctgg
    53641 acttcgtggt gaccatcccc gagggcaagg gaggatggcc ccatttctcc aacctgacct
    53701 ttgccgctaa agacggcaag ggcgtggacg ccaaggtggg gggggagccc ctggatccga
    53761 agaggagcta caaaatggtc accatcaact acctcgccat ggggggtgac ggctacgagg
    53821 tcttcagaga cgccgtggcc cggggaggat acgacacggg attcgtggat gcggcggcct
    53881 tccaggagtt tttggcggat cagaaggtca tagagaactg ggacgacagc cagcgctacg
    53941 tgcgctaggc gccttgggcc ggaggggccc cctaaggggg ccctccggcg ggccgtgagc
    54001 cgaaacgcgg ggaggaaaag gtgaaaaaca cgatgccgcc gggcggtcaa gaagtgggga
    54061 gcgtggcggt ggcaaatcct ctgtggctcg cgccgctggc ggaaatcagt gtgcctgcgg
    54121 tgcgagccct cttcaggagg atgggagtgg ggctcaccca cacggaaatg gtgagcagtt
    54181 cggggttgtg ccacgggaac cgggccacct tggccatgct tccccggagg ggtgaggccc
    54241 ccgtggtgct tcagctcttc ggggacgagg cggaagagat ggggcgaagc ctggagatcg
    54301 ccctgatccg gggggagcgt ccccaggcgg tgcagatcaa catggcctgt cccgtgagga
    54361 aggtcttccg ccggggggag ggggcccgac ttttggagcg tcccgccgtg gcggaggcca
    54421 tggtgcgggc cctcgtgccc tttggcctgc cggtgtgggt gaagacccgg ctggcgggac
    54481 gggggtgtcc cctctccacg ggggacttct gcgacgtgct tttttccgcc ggagcgtcct
    54541 gcgtggcgct ccacgggcgc acggcggccc agatgtacga gggtacgggg gaccgggatg
    54601 ccgtgggggc cgtggcggcg cgctttcccg gccggatcgt gggttctggg gacgtctatg
    54661 cccccgagga tgtccaggac tatctgtccc gggggtgcgc ggcggtgctc ttggcccggg
    54721 ggggcgtgag ggatgtctac agcacgccga agatcctcca ggccctggga tacggcggag
    54781 cgccggaggt cttgaacccc acccccgccc agcgggtggc gactctgatg aatttggccg
    54841 acgatatggc ggaagaagtg ggagaacggg gggccatgat cttgctccgg cgcttcctct
    54901 cgggtatctt taagggactg cccggggcga cctttctgcg ccggagcatc ggcaccgtca
    54961 ccgaccgccc ctcgctggaa agcgtggtgc gaagatggga gcaggtacta gttgagggag
    55021 gcacagacca tggaggatca ggaacggttg caggctgagg gaggatccca ggtgcccgag
    55081 gaggaaatcc tggcggcgcg caaggagaag ctccggaggc tccgggagga ggaggggtac
    55141 gatccctacc gccccgagcg cttcgagcgc agcgcccatc ttgcggaaat aaagcggcgc
    55201 ttcgactccc tgaccgtcga tcagcacggc acggagaagc tcaccacggc gggaagggtc
    55261 atggctctga ggaagcacgg caaggcgtct ttcatcactt tggaggacga gggagatcgc
    55321 atgcagctct acttccagtt cgatgccctg ggagaagagg cctacacgtt cttcaaaaaa
    55381 tgggtggacg tgggggactt cctgggcgtg gaggggcatc ccttccgcac cagccgggga
    55441 gagctctcgg ttcaggtgga gtccttttcc ctcctctcca aggctctgcg ccccatgccg
    55501 gagaagtggc acggcctgaa ggacgtggag gtgcgctatc gtcagcgcta cgtcgacctt
    55561 ttggccaatc ggggcgtgcg ggacaccttc cgcaagcgtg ccaccatcat ggcggccatc
    55621 cgggccctca tgacccgcac cggctccctg gaggtggaca cccccatcct gtcccacctg
    55681 gcgggggggg ccaatgcccg ccccttcgtc accttccaca acgccctggg gctccagatg
    55741 tacatgcgca tcgccacgga gctctacctc aagcgcctca tcgtggggat gatgggtcgg
    55801 gtctacgaga tcgggaagaa cttccggaac gagggcgtgg acaccatgca caatccggag
    55861 ttcaccgcca tggaggtcta ctgggcctat gcggattacg aagacatgat gaccctctgc
    55921 gagaacatcg tcgtggcggc ggcggactcc gtgggggggc gcaccgtgac ctaccagggc
    55981 aaagagatct cgctccatcc ccccttccgg cgggccacca tgaaagaact ggtgaaagag
    56041 catgcggggg tcgattttga cgccatcacc acggacgaag aggcccgaag ggcggcccgg
    56101 gccaagggga ttcccgtgga ggggcacgag agccgcttcc tgctcctcgc caagctcttt
    56161 gaagagtacg gagaaaaaca cctcatccag cccaccttcg tgctccagca ccccgtggaa
    56221 atatcgcctc tggcgaagcg caatcccgag gccccggact acaccaaccg cttcgagctc
    56281 ttcatcaacg gcagcgaagt ggccaacgcc ttcagcgagc tcaatgaccc cctcgatcag
    56341 gaggaacgct tccgagatca gcagcgcaag aaggacgcgg gggacgaaga gtcccacccc
    56401 ttcgacgaag acttcgtcac cgccctggag tacggccttc cccccacggg ggggctcggc
    56461 gtgggcatcg atcggctggt gatgttcctc acggattccc gctccatccg ggacgtgatc
    56521 ctattccccg ccatgcgccc ccgggaggcg tgagcgcggg acggagagcc ctttgagata
    56581 aggaccggcg atgggccagg agtggagaag cgaggtgcga cggtgaacga cgaaaaagag
    56641 ctgcgacgta gagtggaggc gttgcggcgg gagattatcc accatgccca ccggtattac
    56701 gtcctcgacg ccccggaaat cgccgatgac gcctatgacg ccctcatgat agagcttctg
    56761 gccctggaaa gggaccaccc cgagctacaa gacttcggat ctcccacggc ccgcgtggga
    56821 ggagcccccc gggagggttt cgtcaaggtg tcccacccgg agcccatgca gagcctggac
    56881 aacgtctttt ccctggaggg cctcggggat ttcctcgacc gccaacggcg gatcctgccg
    56941 gatctctcgg ggggatacgt ctgcgagctg aaaatcgacg gcttggcggt ctccctgcac
    57001 tacgagaacg gggtcttcgt ccggggggcc acccgagggg atggcaccgt gggagaagac
    57061 gtcacggcga acctgcgcac ggtgcgcacc gtgcccctga cgctgaggga gtccctcccc
    57121 ggggccctgg aggtacgagg ggaaatatac atgcggagcg aggatttcgc cgccctcaac
    57181 gcggagcggg aggaggccga agagcccctc tttgccaacc cgagaaacgc ggcggcgggg
    57241 gccttgcgcc agttggatcc cgccgtgacg gcgtcccgca ggctgtcgtt tttcgcctac
    57301 gccgtgtcct tcccggaaag gcggggtctc gccacccaaa gagagctctt ggaggccctc
    57361 cggcggtggg gtttcgccac ccagggaaac gaagtcctcg cttcgaacct gcgggaagtg
    57421 ggggcctacg tggaacggtg gcgggagggg cgcttttccc tgccctacgc caccgacggc
    57481 gtggtggtga aggtggatcg actggagctc cgagacctct tggggagcac cgcccgggcc
    57541 ccccggtggg ccgtggccta caagtacccc cccgaggaga agaccacccg ggtgcgggac
    57601 atcgtcattt ccgtggggcg taccgggagt ctcacccccg tggcgatcct ggatcctgtg
    57661 cccttggcgg ggacggtggt gcagcgggcg agcctgcaca acgccgacga ggtggcccgc
    57721 aaggacgtga ggatcggaga ccgggtggtg gtgcgcaagg ccggggagat catccccgag
    57781 gtggtgcggg tggtgtccga agaccgttct ggcaccgaga ttccctttgt catgccccct
    57841 cgctgccccg tctgcggcac cccggcggtg cgcctccccg aggaggccgc cacccgatgc
    57901 ccccgccgat cttgtcccgc ccagatcaaa gaggggctgc ggcacttcgc ctcccgaggg
    57961 gggctggata tccggggatt gggagaaaaa atcgtggatc gcctggtgga aacggggcgg
    58021 gtgaaggacc tggccgacct tttcgacctc tcggcggcgg agctggcctc gttggaccgc
    58081 ctgggagaaa agtctgcggc caacatgatc caggcgctgg agacatccct ggggcgcccc
    58141 ttgccccggt tgctcaccgc cctggggatc cgccacgtgg gcagtcgggt ggcggagctt
    58201 ctggcggatc gtttccgcag cgtggaggcc ctcacccggg ccacggagga agaactcgcg
    58261 tcggtggatg gagtgggccc ccgcatcgcc gcctcggtga gggccttcct ggactctccg
    58321 gcaaaccagg aaaccctgcg gcgcctggct tcccggggca tggccctggc ccttcccgag
    58381 cccgaggccc ctctcgccga aggaccctgg aaggaggagc gggtggtgtt caccggcgag
    58441 ctccggacgc tcacccgcac cgagggcgag gccctggtgg cctccctggg ggggcgggcc
    58501 ctggggagcg tgagctccaa gaccaccctg gtggtggcgg gggaaaactc cggcagcaag
    58561 cttcgcaagg ctcaagagtt gggcattgcc gtggtggacg aggaagagtt cctggcccgg
    58621 gtgcggcggg cgcgaggtga aaagtctcct ggcccccgag acggggcgtc gtagaggaag
    58681 gctgctccga aggtttccaa agcgccaccg gcgtcggcgg cggcgctttt ttcccggaag
    58741 gactccggtg ccctacgggc gtttaggccg cccccggatc ggccggggcg cgggatgcgg
    58801 ttcatcggcg aaaagggggg ggagatttca tgtcaggtag ggcctacgcc ctggccctct
    58861 cggccatcgg cgtgtggagt tcgttggctt ttctggggtt gcgtctggca tccgttcctc
    58921 ccttcctcct ggtgggactg gccctgctga tcgggtcggg gtgtagcgtg catcactttc
    58981 gtcagtggcg cgtatctccc aagcttctgg ccctgggggt ttacggcctc ttcggctatc
    59041 acctgtgctt tttcctggcc ctccgcctgg cccctccggt ggaggcgaat ttaatgaatt
    59101 atttgtggcc cttattcacc gtgatctttt ccccttggat cctgccgggt tactctctga
    59161 ggccccacca cctgggggcg gccttcctgg gcatgacggg ggcagccctc atcgtcaccg
    59221 gggggtcttt tgtgagcttt cggagcgagt ttttgccggg ctaccttctg ggagggggag
    59281 cggccttcat ctgggcgacc tattccctca cgacgaagcg cctggggggg ttccccaacg
    59341 ccgccatcgg gggattttgc ttcgcctccg gggtgctgtc cctgctggtg cacgtccttt
    59401 tggaagagcc ctatatcccc accctgggag aactcccctc cctggttgcc ctggggctgg
    59461 gacccatggg aggggcattt tttctgtggg atggggcgtt gcgccagggc gatccccgga
    59521 tcatcgggtc cttggcctac ctgacgccgc tgctatccac cctgctattg accttttcgg
    59581 ggcgaggcga gtttactccg atcgccgccg gggccatggg gtgcatcttg gggggagcct
    59641 ttctgggcac ctgggggact cgaggaaagg gcgccccttc tccctagggc ttttggccgc
    59701 cggggtaccc tcgggtcttc cggaggaggc gggggggaag accccgtact ttttgggaac
    59761 gaggtgacgt catgcacggg agatcggagc cggaagggat cgtttcgccg gaggaggtgc
    59821 ggcgcatcgc cgccctgatt cagttggagg tgggcgacga cgaagcggag gacctgggga
    59881 ggcatttcca gaaaatcctg gggtattttg cccgccttcg ggagatggat ctggagggag
    59941 tggaaccctt taccccggga gagcctagca tcgccctccg agaggacgtg gaaacccctt
    60001 gggaggagag gggagaggtg ctggatcgag cccccttccg ggagggggat tttttccggg
    60061 tgccccggat ggagcgggac gaggagtccc gaagggaggg cgaaggtcga gccggatccg
    60121 gcgaaggggg acatctcccg ccggggtcgt gctccgccga ctgagggagg aacgacatgg
    60181 aactctggga atattccgct tggcgcctgg cggagtttgt ggcgcagggg cagctgtccg
    60241 tgagcgaggt ggtggaggcc cacctgacgc acatgcagtg gcaggagggg acgtaccacg
    60301 ccctcatcac ccccatggtg gaagccgccc gggatcgggc ccgggccctg gataccgccc
    60361 tggcaggggg ggcggacatc tccccgctct gcggtgtgcc ggtggtgata aaagacaaca
    60421 tctgcgtcca gggatctccc accacctgcg gatctcgcat gttggagcgc tgggtggccc
    60481 cctataacgc cacggcggtg gaccatctcc tgggggccgg ggcggtggtg gtggggaagg
    60541 gcaacatgga tgagttcgcc atggggagct ccacggagaa ctccttcttc ggccccacca
    60601 aaaacccccg ggatccgaac cgggtgccgg ggggaagctc cggggggagc gccgccgccg
    60661 tggcggcggg gtacgccccc ttggccctgg ggagcgatac gggggggtcc atccgccagc
    60721 ccgcagcatt ttgcggcgtc cacggtctga agcccaccta cggcatggtg agtcgccatg
    60781 gcctggtggc ctatgccagc tcctttgacc agatcggccc cttggcccgg gacgtggggg
    60841 acctggcggt ggccctggag gtgctgggaa gggccgacga ccgggatccc acctgctctt
    60901 cccgtcctag gaccacctac ctcaacgcga tacagactta ctccctgaaa gggcgccgca
    60961 tcggcatggt gcgggagttc gctggcagcg cggtggatcc cgagctccac gcggccatgg
    61021 tgcacgcggc ccggatctgc caggacgcgg gggccaccct ggtggacgtg tcgctccgtg
    61081 acaccatcgc ctacggcctg gcggcctatt acatcctggc tcccgccgag gccagctcga
    61141 acctctcccg atttgacggc gtgcgcttcg ggttctgccg ggaggccgaa gtccttcagg
    61201 aacagtaccg gcgcacccgg ggggaggggc tgggccccga ggtcaaacga cgcattctca
    61261 cggggaccta cgtgctaagc tcgggatatt acgacgccta ctatgccacc gcccagaagg
    61321 tgcggcgccg ggtggcggag gagttcgatg tggccttcca atcggcggat gtggtcttgc
    61381 tccccacctc tccccggctg cccttttact tcggggaagt gggggacccg atggccatgt
    61441 acctcatgga catcttcacc gttccggcga acctggcggg gcttccagcc ctgtccctgg
    61501 cgacgggatc gggacgggac ggcttgcccc tctccgtaca gctcgtggga aggccctggg
    61561 gggagttcga cctgttggga gtggcgtcga tcctggaaaa ggccttcggg gcgccgcaac
    61621 cgatccaggg gacggggggg aaatactcat gagagaagag ctttctccgg tcatcggcct
    61681 agagatccac gtgcagctcg ccacgggcac caagatcttc tgcgggtgct ccacggacta
    61741 catcggagcc gtgcccaacg tcaacgtgtg ccccgtgtgt ctgggccttc ctggagcctt
    61801 gccctcattg aacgcccggg ccgtggagct gggaaccctg gcgggattgg ccctggggtg
    61861 tcgcatccgc ctccggtctc gctttcaccg aaagaactat ttctatcccg acttgcccaa
    61921 ggggtatcag atcagccagt atgacgagcc tttggcggag gagggattca tcgacgtggc
    61981 ggatgagaag ggcgggacgc gccggatccg catcctccgg ctccacctgg aagaggatgc
    62041 gggaaagctc gtccacggag cgggagacgg ccgtctggag ggagcatcct gctccttggt
    62101 ggactgcaac cgggcggggg ttcccctggc ggagatcgtc tccgcccccg atctcacctc
    62161 tccggccatg agccgggagt acgtcctggc cctgaggcgc ctggtacgcc acctgggagt
    62221 cggggacgga gatatggagg cggggtcact ccgggtggat gccaacatct ccctgaaggg
    62281 gccgggggag tccgggggag agcgggtgga gatcaagaac ctaaactccc tccgggccct
    62341 ggagcgatct ttggaatacg agatttcccg gcagagagat ctgatctccc gggggggaac
    62401 agtggcccga gaaacccggc attggaacga ggtcacggag cgcaccctgg ggacccggtc
    62461 caaggaggaa gcccaggact accgctactt ccccgacccg gacctgcccc ccttggtgat
    62521 caccgagggc ttcgtggagg cctgtcggcg gcgcctaccg gagcttcccg acgcccggcg
    62581 tcggcgtttc gtggagggcc ttggcctttc ggaggaggag gcggccgtcc tggtggagga
    62641 acggggcgtg gcggcgtatt ttgacgccgc cgtggcggcg ggggctcccc ctcggcgggc
    62701 ctcggcctgg gtgcgcaccg aggtgctgcg ggtccttcgg gaggagcgcc gggcggtggg
    62761 ggccttcccc gtgctgccgg acaccctggc ggccctcctc cagagggtgg aatccggaga
    62821 gctctccacc actgtggccc gggagtgctt ccacgccctc cgcaccgaag agatatcctt
    62881 ggatgaggcg ctgcgccgct ttggcggagg aaagccctct tcggaggagc tcgaagccct
    62941 ggggcgtcgg accctggagg agaacgtcga cgtggtggaa gagatcctcg gagggaggga
    63001 tcccaagggg aaaaagatca agtacctgga ggggttgatg atgcgggccc tccggggaca
    63061 ggagacccca gaggcggtgc ggcgcacgct ggaggagctt atttttaaaa aataacaaag
    63121 tgtctatcga tgttttggag gaaagatcgt cggaaaggat gagatgtttt gatacaaaac
    63181 gtcatttttg acctgggggg cgtgttgctt aagtgggagc ccctggagta cgtccatcgg
    63241 accctggggg gaggggccct ggcagagcgg atttgtcgag gagtgttgga ggccccggag
    63301 tggcaagacc tcgaccgggg ggccctggag gaggacgagg cgctggagcg ctttctccag
    63361 cgtttgccgg aggataaaga ggcggtgcgg cgcatcttcc cgggcctttt cgaggagttg
    63421 cgtcccatcg aaaagcacgt ggccctgcta cccctcctca aggcatcggg aaagaggctc
    63481 ttcgtccttt cgaacttctc ccgccggggg tacgccttcg tctcctcccg gtacgacttt
    63541 ttccggcact tcgacggggg agtggtgtcc tacgaggtga agctcctcaa gcccgagcgg
    63601 gagatctacg aagccctcat cgatcgatac gccctggacc cctcgacctg ccttttcttt
    63661 gacgatcgag aagaaaacgt ggcggcggcc ctggaccagg gcatcgacgc cgtggtggta
    63721 cgccatgagt cccaggatca ccggggcatg gcctggcccg aagtggcgtc cgaccgagag
    63781 ctccccggca ctttcgacct gagggaggag atggggcgtc gggggctcct atagggaagg
    63841 gggccggaag agggttacct gaggataacc cagggagggg gcgatggagg ggcgaagggg
    63901 gcccgaaggg atccggcgag atcccggagg ggagggagga acggccggtc tgggtatcga
    63961 agggggtttt ttcatgcaag acatctccgt cagcaccgtg gcagtggtga tccaggtgtt
    64021 gctctacgcc agcgtggcgt acatcgcgct gaagcggttt aagttcccct acaccatcgg
    64081 gctggtgctg atcggcctga tcatcggagc cgtggtcaac attttcggca tcctaccagc
    64141 cttttctcgg cttcacctca cgggaaacct catcctctac gcccttttgc ccgccctcat
    64201 ctttgacgcg gcgctcaaca tggatgtcca gctcctaaaa aagaacatcg tgcccgtgat
    64261 ggtgttggcg gtgccggggc tggtggtggc cactctggtc tccgcctggc tcgtcgccgc
    64321 ctggaccccc ctcggcatgg gggcggctct ggtcttcggg gccctgatct ccaccacgga
    64381 ccccgtggcg gtgatcgccc tgttccacga gctgggggcc cccaaacgcc tcaccatgtt
    64441 ggtggacggc gagagcctct tcaacgatgc cacggccatc gtcatgttcg ccattgtgct
    64501 ggggctggtc tcctcggggg cgcctttttc cgtgggcacc gtgttgttgg cggcggtgga
    64561 gttttttctg gtcttcatcg gggggcttat ggtgggagtc gccgtggggt gggtcgtcat
    64621 ggggagcatg cgcttcgttc gcgccgaccc cctggtgcag ctcacgctca ccatcgtttc
    64681 tgcctacctc tcctttttgc tggcccaggg ggccttgggg ctctccgggg tcatggccag
    64741 cgtgggggcg gggctcgtca ccgcccatta cggtcgggtg tacttttccc gggaaacccg
    64801 ggattacctg caccatttct ggtccttttc ctccttcgcg gcgaacagtt ttgtctttct
    64861 cgtcataggc ctcaccgaag tttacctatc ccggggtcac ggttttggcg ccacgctgtt
    64921 ctacgccctg gcggccatcg ccgccatcac actggcccgg atcctcatcg tgggggcctc
    64981 cttcggcatc ttgaacctga cccggaactt tcaccgggtg aactggcgcg agcaagtggt
    65041 ggtggttctc gggggactcc ggggagccct tcccatcgcc ctggcggtaa gcattcttcc
    65101 cgccgaggtg gggggagaag agaaccgacg cctcatcgtg gactttaccc tggcggtggt
    65161 gcttttcagc ctgttggtgc agggtaccac catgggattg gtgttgcgcc gtttgggcct
    65221 catgaaggaa gacctggtcg gaaggatggt ccgcctgcgg agcgccatcg gcgcctggcg
    65281 tcgcacggcc cgagccctga gctccacggt ggagggggag gagacctccg tacccctctt
    65341 cgacgaggtg cggcgacagt acgaggcggg ggttcaaagg atccgggggg aattgcaaaa
    65401 catcggcgaa gaaagccccg aggaagcccg ccgagtagcc tccgccctca cctggcgcta
    65461 tgtcctggcg tggatgcgct cctcggtgga gttcatgcat tccgccggag tgctggacga
    65521 ccccagcgcc ctggcgattt acctgcagat cgaagaggcg gcggatcgaa acgaccatgg
    65581 ggagaccccg actctggcct ttcgcgagct ggaggcgtcg ggaattcaaa gggccttttt
    65641 ggcgcttttg cgccgtcacg cccccgactc ggagttcttc cggaactccc aggaagaaag
    65701 gacgcttttc atgtccttgc tacgtcgtgg ggcccctcgg tctctggtct acggagagct
    65761 ccgacgggag gcggaaagga gccctctgcg ccacgccctg gggtgcatgg ccgtcgccgt
    65821 gcgggcccgg cttcgggtcg ctgaggtcca agagatggtg gaaatggatc cggaggtggg
    65881 agagcccttg atggcggccc ttcagggctt cgcggaagag gcccgggcct tcgcccagag
    65941 gtatcaggag acccacccgg atcacgccga cgaggtggtc tggaacgcca tggagcgcta
    66001 cgcggcggcc ctggcccggg gggccgtggg agaactgaag ggttggggaa tgatcgacgc
    66061 cgccggggcg gccctcctgg agtccatgcc ttccccgttc tcggtggaag aggcccgcgc
    66121 gggggcggag aggggatagg agacactccg tgggcgacgg ggcctttccc caggggaagt
    66181 cttcggtgcc gtcgggcctg gcctcaggcg ggtaggggtg gtatattatc atcgcggtgg
    66241 tctcggagcc tccttagggt cgggggcttc gggccccggg aaatcaaaac tcccgacagg
    66301 gaaaagaaag gacagtggta ggagatggtg cgaggtttcc gggcggtttt tttagtcatg
    66361 gcgctgattt tggcggtggg gggaggggcc tgggcggaag accccgttaa gggaggaacc
    66421 ctgctgtggc gcgatccttc ggacgtgccc accctggatc ccgccaaggt caccgataac
    66481 agttccactc ggctggctct caccatcttc gagactctgg tggaaaacga cctggagggc
    66541 aagggagtgc tcccctgtct cgcggagcgg tgggaaggga gtgacggagg caaggtatgg
    66601 accttccatc tgcgcccggg ggtgcgcttc caggcccaag cggagggaca gcccacggcc
    66661 aatggaggcc gggaggtccg ggcgtcggac gtcaagtaca gcatagagcg cctgatcctc
    66721 accgactccc cccgggcgta cttcctggac atggtaaaag gatatcagga cttcatgagc
    66781 cgggatgtca ccggggtcac cgagtgggcc ggggtcaaag tcatcgacga aaagaccgtg
    66841 cagttcgagc tggactaccc ctttgcgccc ttcgtggcga tgctcgccta ccccaccttt
    66901 tccgtggtgc cccaggagga cgcggagaag tgggggaagg actttggctt ccaccccgtg
    66961 ggcaccggag cctttgcctt cgaaaagtgg gagcatgata tccgcatatc cttgaagaag
    67021 aaccccgact actggcgcac cgacgcccgg ggtgagcgct tgccctacat ggatggggtg
    67081 gaattcgtgg tggtccccga cgacgctgtg gcctacgaag agctcaaaaa gggcaacctg
    67141 gacgtcttct cctacgttcc cgacgagatc taccaggatg cccgggaaaa gttttccgac
    67201 accctcaagg agagcccggc cctggcggtg tcctactacg gcttcaacat ggccaaggag
    67261 cccttcggat ccaataaggc cctgcggcag gcggtgaatt acgccatcga caagaacaag
    67321 ctcaacgagc tgatctacga gaaccgggtg gtccccgccg tgggggtact acctccgggc
    67381 atgccggggt acgacgtgac ggacatgaag ggctatccct acgacctcga taaggcccgg
    67441 gcgctgatga aagaggcggg atatgagaag ggactggagt gcactctcca gatcaacaac
    67501 gtccagcgcc accagcgcat cggcgaggcg gtgcaggcca tgctgatgga agtgggcatc
    67561 accatgaaca tcgtcactgt ggactggggc acgcacatcg acgccctgga ccggggagag
    67621 gtgcccttct tccgctactc ttggatcgcg gactacgccg atccggacaa cttcctctat
    67681 gtgctcttcc actccgccaa cgcgggaccc aagggaaaca actgcttcta taacaatgcc
    67741 gaggtggacc ggatgttgga ccaggcccgg gtggaagtgg actggaacaa gcgggccgcc
    67801 ctctaccacg aggcggagaa gatcatcgtg gacgacgccc cctgggtgtt cttcttcaat
    67861 ccggtgaacg agctccttag cgtgccccgg gtgaaaaaca tcaagctccc cacctttggg
    67921 gcggattggg cgccgcttta cgacgtgtgg ctggcccagt gaggcacctc tgatatcagg
    67981 ggatttagcc cgggatggcc ccgggggtct tttgaggggg cttttcccgt cgcgcctaac
    68041 cctactttcg cacaaaaaag acctgagcct ttgcgaatac tgattctcac caaaaagtta
    68101 ggcactacga gatgccctct ccctcggaga aaaagatcat cttcgtagtg ccctttttgt
    68161 ttcatccggt gggcggaatt ctttcgctgg agggccgccc ggagccctgg ttccgtcgcc
    68221 ttcggtcagt cgctccggtc atcctccggg ggaaggtctt ggtcgtggag gatggaacgg
    68281 gagattccca cctccacttg ggactccgtt ccggtcccca tcggggcctc gcagggtcct
    68341 tcccgtacgt gctccatagt tttcggaagc gtgctgcctg tttgtacgac gatgatcggc
    68401 gccatgcgct ctcattcttt attaaaaaac tcaaaaggat tgcggggctt tgggacgacg
    68461 aagatcctag aagaggtccc agatctgttt gcctatgagg accgtggtga cggaaagaaa
    68521 gagggggcgc acgtaggcca ctcccttggt cagggccagg cgcgtgcccg ccagggcccc
    68581 cgctaccatg ccgatgccca tgacgagccc cgccgtgtat tggacggagt ccaacagggc
    68641 gaaggtgacg atggcggcga tgttactggc aaaattgagc acccgggcat tcccggcgcc
    68701 ccggacgaag tcgaaaccga gcatcaaaaa gacgaacagc agaaaagacc ccgtgccggg
    68761 gccaaaaaat ccgtcgtaga agcccagacc tagggacgcc aagcctcccc aaacccatac
    68821 ccggggcgtg gggcccgcgt aggtggcgtg ctgcccccat tctttgcggc acaccgtgta
    68881 tagcgccacc agcacgagca tgaccaacac cagggggcgc aaaaagtccg ggggaacgcg
    68941 gcgcaccgtc aggaccccca gcaccgatcc ccctagggaa aggggaaaga accagcggac
    69001 gatctccatc tccacgcggc ccgatcggag gaaggcgagg gagctggtaa agctggaaat
    69061 gaccccggcg agcttgttgg tgcccagggc cagctgggga ggaaggccca aaaaaagcag
    69121 cgaggggagg gagatgagtc ccccgccgcc caccacggaa tctacgaagg aggcaaaaaa
    69181 tcccgccacc acaagaaaaa gggctgttcc ccacgtcaaa ggctccaccg ggccaccatt
    69241 cctctcctag cgactcatcc tgtttgggcc tcttccggga taaagggggc ccaggccttc
    69301 gccccccctc ccacaccatc aacatacgcc ggaaggccga ggggcacaag agccaaggaa
    69361 ggggaaaaga tcaaagcctt tcggcgctct gggggcgacg ggatccttat gccatgggcg
    69421 gtcgatcttc ccggggagta ggggctaatc gaaagcccga ggcgaaaaag tctttctttt
    69481 gtctcgtttt ctcgaggggc cgcttgacaa tacgtagtag gggcttatat atgggccatg
    69541 tcccccccga ggggccccct ggtttagatg gcggcgaggg cctttgctcc ggagagggga
    69601 cgccttcccc cggggggggg gcgaaaaacc gggtaacgga aaggagtgcg ggatggaact
    69661 taacctgtgg cacgtggggg ccatcgtggc ggtgggattg gcccaaggtt ttttgaacat
    69721 cgtggcggga gggggggcct tcttggtttt gcccatgctc accttcctcg gcatggactt
    69781 ggcgatggcc aacggcacaa accgcatcgc catcctgctc cagagcgttt ccggggcgac
    69841 gagtttccgt cgggacaaga tcctatcctt ccggtcggcc cttcccctgg ccctggtggc
    69901 gacgctgggg gccacggcgg gggcctttac ggcggtcaag gtggataagg ccaccctgaa
    69961 catcgtggtc gctgggctca tctccgtcat ggcggtgctc cttttgggaa aaccggagat
    70021 gtgggagggc cggaaggatc gccgatggtc tcgatggatg acctatgccc tcttcgccgg
    70081 gataggtttt tatggcggat tcatccaagc cggggcgggg ctttttatct cctgggggct
    70141 cgccgccgcc gccggggtgg acctggtgga gggcaacgcc atcaaatcgg tgatcatcgg
    70201 atgctacacc gccgtgagct tggcgatctt tttcgccaac ggcatggtgg atcccccagt
    70261 ggggctagtg cttgccgtgg ggagtgtggt gggggcctgg atcggcgcca agttcaccgt
    70321 ggccaagggt aatcggtgga tccggcgtat cctggtggtg gtggtcatcg tcagcgcggc
    70381 gaagatgata ggggacgtct ggtaggatcg gggcctgggg ttcggcgacg aggggagagg
    70441 ccccggtcga aggagccccg aagggacgac atgcgccact tgaggaggtg ggaaaccttg
    70501 atttcttctg attattccga tcgaaaagac cttgccgggg gtcttccctt tctgggcttc
    70561 gacccggagt atcaccgata cccttcccgc cgggcagtgc tctacgcttc ccgggggatg
    70621 gtgtgtaccg cccacccgtt ggcagcccaa gcggggctgg aaattctaaa aaaaggcggc
    70681 aacgccgtgg atgcggcggt ggccacggcg gcggccctca cggtggtgga acccacctcc
    70741 aacggcatcg gcagcgacgc cttcgctctg gtgtggaaag acggccgtct ccgggggctc
    70801 aacgccagcg gcccctgccc cgggagcttg tctctggaca ccgcgcggcg ggaggggtgg
    70861 agcgccgtgc ctcccctggg atgggccccc gtcaccgttc ccggagctcc ggcggcgtgg
    70921 gcggagctca cgtcccagat ggggcgtctg tcgctgcggg agaacttggc tccggcggtg
    70981 gcctacgccc gggaggggca tgcggtctcc gtgacggtgg ggcgctgcat ggcggaatcg
    71041 ttccagcggt acgcccggga atcgggggag cttttcgctc cttggtttga gacctttgcc
    71101 cctgcggggc gtccccttag gatcggcgag cggtgggcct cccgggatca cgccgagacc
    71161 ctggagcgca tcggggacag cgacgcccgg gacttttacc ggggagagct ggcggagcgc
    71221 atggtggccc acgcccggcg caccggggga tttctgaccc tggatgacct gggggccttc
    71281 gcccccgagt gggtggagcc cctgcatggg gcctacaaag gttttgacgt ctgggagatg
    71341 ccccccaacg gccagggcct cgtggcgctg ttggcccttt ccatcctccg ggagttttcc
    71401 tttccggacc accacgaccc cctcacggtg caccgccaga tcgaggccat caaaatggcc
    71461 ttcgccgacg cccatcggta tgtggcggat ccccgatgcg cgccggtgcc cgtggaagaa
    71521 cttctttccg aggagtacac ccggtcccgg cgggccctgc tccgctccga ggcgggggac
    71581 ttctcggcgg ggaaccccct tcccggagga acggtgtacc tctgcaccgc agacggggag
    71641 gggaccatgg tctcctacat ccagagcaac tacatgggct ttggctccgg ggtggtggtc
    71701 cccggtaccg gcatcgcctt gaacaaccgg gggcactgct tctctctgac cccggggcac
    71761 cccaacgtcc tggcgccggg aaagcgcccc tacaacacca tcattcccgg ttttctcacc
    71821 cgccatggcg aggccctggg gccttttggc gtcatgggag ggtttatgca gccccagggg
    71881 cacgtgcagg tggtgatgaa caccgtggac ttcggcatga acccccagga ggccctggat
    71941 gccccgcgct ggcagtggat ggggggcatg aaggtctccg tggagcccgg atttccttcc
    72001 gccctggccc aggccttggc ccgcaaagga cacgcggtga cgtgcgaacg ggaatcccta
    72061 tcctttggtc ggggagaaat catctggaga acggatcagg gaacgctggc gggggccacg
    72121 gaaccccggg cggatggagg cgtggccccc tggtgacggc gggagaatca aagggcctac
    72181 ccctcctttg gatcttccgc gacgggcgcg gccttatcca tgaacgcccc ctttcctagg
    72241 ccggaaaggg ggcgtttttc ttcggttccg gcggggacaa agattccaat aatcggcctt
    72301 ctcagttctt ttgggcgttc tcggggaacc ttgatccgca ggtaacagga agagaagaca
    72361 cgcaaaaccc ctctggtgcc gcttgaacag cggaagtaag gttaaggcgc agaccaaaaa
    72421 cctcacacca aaggggtgcg tatattatgg gggcaaaaca agggaactgt cactacgaaa
    72481 tagagcggaa tcagcgggca aacctgacaa gtcgaggttc cccggggaag attgcctctt
    72541 gtttttgaca gaaaaagtgc tattgtacta caccgcactg cggtgcacgg tggatgtggt
    72601 ggtagggtag ggagagagcg ttcgagcctc tcaggcaatg gacgcggaga cgagcggtga
    72661 ctgtggagaa agagggaatg ggccatgcgg aacacgacgg gcaccacgtg gcgggtcctg
    72721 ggggggctcc tcggggcggt atgggtcctt tggggaagtg cggggggagt ctgggcggac
    72781 aacgcctttc tttccacccc ccgggtgtgg gtgaacggtg cgtggcacaa gtatgggccg
    72841 agcctgggag gcatcaacgc cgcagtaacc aagacggtct tcgacatgac catcagttac
    72901 cacgacaccc tttccgccga cagcgtcccc ccgggggacc ccaagcggac ccgggccctg
    72961 gaagtggagg aggtcatcaa gttctggtcc gacgcgctct acgagcagtc caacggccag
    73021 cacatgctgg gcacggtgta catctaccag gggggcaaga tgggtaataa ggcctccgtc
    73081 ctctgggtgg ccacgtccca tccctcgggc ctccccggag gctacggcaa gcccctggga
    73141 aacatcaact ttggcgatgt cttcggcacc aacgtcttcg tgggggcgac gagcaccgcc
    73201 acttcccagg ccaacggggg atacaccctg gggcacgaga cgggtcattt cgtctacgcc
    73261 atgtacgacg agtacagcgc gaactacgcc ccggcccact tcccccgttc ccgggacgtg
    73321 tccccttcga gtcccgccat catgaacgac cagtgggggg ccatgcggga caatcaggtg
    73381 cggtggctca atcactccac ggcggggaac tacaaggcca gcaccgacac cgcccagaag
    73441 cgggtctacg gcaagagctg ctgggaagtg atgctccagg atccttcgct ggacggcccc
    73501 aatgcccgcc ccgtggcggt gtggccccag cgggtccagt acgccttcgg caatcccccc
    73561 gcgtcgccgg attggtaccg cacggcggtg acgccgggaa cgcacctggc ggcggcccag
    73621 gcgaatctgc gcattgtctg gctttcgggg ggcatggtct accagatcat catcgaccgg
    73681 tcgggaagca tggtccccga tcccgatccg gtgaaccccg ggccctctcc cttggacaac
    73741 gccaaggggg cggcccggga tctggtggag tccatggcga aggagaccgc tgtgggggtg
    73801 gtgcagtttg acgacggggt ggcccaggtc taccccatca ccttcattcc gtcggacgac
    73861 gtgggagccc gggcggtgaa agacgccacc aaggccgcca tcgacggcat ttccaaggga
    73921 gggttcacgg cgatctacga cgccgcctcc acggcgctgg cgaacatcaa gacctatcag
    73981 gggacggtga gcggtgatct gacgggggcc tgctatctcc tcaccgacgg ggacgacaac
    74041 agctcgtcca agtcctcggc ccaggtcatc gcggagtacc aggccgccaa gattcccctg
    74101 gtgacggtgg ggtacggctc cggcggagcg ggaaaccccg tgctttcttc cctggcggat
    74161 gaaacggggg ggcagtactt cacgtccccc accacccagt cggagctcca gcaggtgttc
    74221 ttcgccgccc aggggagcta ctccgacgtg gtgaacctcg gcaatgtcaa gaagaccatt
    74281 gctgcggggg tggcggatca ggccctcttc cgggtggaca gcaccatggg gagcgttaag
    74341 ttcttcctga actggaacgg gacttcggcg gacctgacga tcaccgtgag aaatcccggg
    74401 ggtattgcgg tgcccgtgac gccccagatc tcccagacgg ggggagcctc ggactactcg
    74461 gcggaggtga aagctcccac cccggggctc tggacggtgc atgtccagaa caccgccgct
    74521 acgccccggg aggtctcctt ctccgccgtg gggcaaccgg tggaggggac gggctttgcc
    74581 ctgacggtgg gattggcgaa cgggggcagc acggtgacgc ctcccgatcc catccgcctc
    74641 ttcgccacgg taagcaaggg gggtgtgccc ctggcccacg ccacggtggt ggccaccatc
    74701 accgcccctt cgagctccac gcccacactc ctgacgttgc gggatgacgg cacgggggga
    74761 gacgcggtgg cgggggacgg cacatactcg gcgctccttg ccagttacgt ggcgggaacc
    74821 tataagatcg acgtcaccgc ctcgaacccc tcgggcacgg cccgggaatc cttctggagc
    74881 tactctccct cgcgccccgg tccgggggac gaaggactgg tcttcaaccc cgacggtgcc
    74941 ctggcggggg aggacttcca gcggagcacc tcgttccagg tgaaggcttc gggaggagtc
    75001 acccccacgg cggccccgac gcccaccggg gctcccaccg tggcgccgac ggtggctccc
    75061 accgtgatcc ccacggcggg gcctggtggt ggcggcggtg gtggatgtgc cacggggatg
    75121 ctggcgttcc cctgggcggt cgtgcttttg gtgccgcttt tgggatgggg gcgaaagccc
    75181 taaaggggaa gatggtggcc gttgaggctc aaaacgaggc tcaaaaaacg gtggtcaccg
    75241 cgatagcccc gaagaaaacg gcttcacgat gtgcggaaaa gggggcgctg ggcgccccct
    75301 ttttttatcg gaggcggggc tttttgtgcg ggcgtcatct ggtgcagggg gacgtctccc
    75361 tgctgctctt tgatgtcacc accctttact ttgagagtt
//
LOCUS       DAOOER010000003        74439 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_350_length_74439_cov_7.931733, whole genome shotgun sequence.
ACCESSION   DAOOER010000003 DAOOER010000000
VERSION     DAOOER010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 74439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 74439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74439
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_350_length_74439_cov_7.931733"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            306..1250
                     /locus_tag="PL162_00640"
     CDS             306..1250
                     /locus_tag="PL162_00640"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HQF90568.1"
                     /translation="MVSAIFRGKATLGFFLGLVALLALFPLTKSWAVGMALFLGAAVG
                     NVRPSAVPPELGSWRKITLNTAVVLFGFGLDIRQVLAVGSQGFWQTAVSLGVVLALGF
                     ALRRFFSLPPATTKLITVGTAICGGSAIAAVSSVMKARDEEVGVAMGVVFLLNTVALL
                     AFPLLAKMTSLSPEQYGVWCALSIHDTSSVVGAAAFAGDGALSTATILKLTRTLWITP
                     LVFALSLLEGGKGKFSVPPFILLFLLASIIASALPFAPLFGALASLGKVFMAEALFLV
                     GLGLRAAALQRAGWRGVLFGGALWAVSLGVGFGLASGS"
     gene            1331..1999
                     /locus_tag="PL162_00645"
     CDS             1331..1999
                     /locus_tag="PL162_00645"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQF90569.1"
                     /translation="MDDYYAKHLHATRLLLVYQTDIERVRQYLDAEIAFVRGHLTGSE
                     RVLELGAGYGRILRELAPFAASLVGIDIAEDSVALGEEYLRDVPNCALRTMDAHHLAF
                     QAEFDVVLGLQNGLSAMKGDPLHLVRESLRVLVPGGRAFFSSYSPRFWEHRLAWFAEQ
                     AEKGLLGPLDWEKTRDGVIVCQDGFRATTFGAEDLERLAQGVGCPYHIEEVDDSSVFL
                     VLEK"
     gene            complement(2173..3363)
                     /locus_tag="PL162_00650"
     CDS             complement(2173..3363)
                     /locus_tag="PL162_00650"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HQF90570.1"
                     /translation="MKLSHRALRMEPSATLAVAGKAKALAAQGKSVISFGAGEPDFAS
                     PPSALTAAQEAMAQGKTHYTASSGIPELKKAVAAYYQERFGLTYAPKQILVGAGAKPL
                     LYEALGCLVNPGDEVIIPAPAWVSYLEQVNLFEGKPVVVETAPSGYLPTLEALEKAVT
                     PRTVGLLLNSPCNPTGQVYGEELLRGIGELAERHNLWIIWDEIYERLVYGDARHINPL
                     ELLPHLAPRVLLVNGVSKAFAMTGWRIGYALGPEALITKMDEFQGHLTSNACSVAQWA
                     SLGALQGAEADVVRMHQAFASRRELMMSRLRSMPHISFPEPRGAFYVLVDLRSCLGLQ
                     SATRRLDDDSAFCEALLEEELVAAVPGSAFLAPGHLRLSYANATEEITEGMNRLERFL
                     KSLK"
     gene            3621..4169
                     /gene="raiA"
                     /locus_tag="PL162_00655"
     CDS             3621..4169
                     /gene="raiA"
                     /locus_tag="PL162_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HQF90571.1"
                     /translation="MDIRYVLKNLELPDVVRDVMEKKLGKLEKFFDPILDTQVEVQHR
                     RGMFVVEITSNVNGIIMRGEDYAPDIRKAFEKSLKNIERQIKRHKDALKDHSRTKEAL
                     AEISFDIESILPAEDEGAGEKGTIVKVKRFPFRLMSAEEATMQMDLLGHSFFVFRNAD
                     SGETNVVYRRHEGGYGLLQPDK"
     gene            4284..4625
                     /locus_tag="PL162_00660"
     CDS             4284..4625
                     /locus_tag="PL162_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HQF90572.1"
                     /translation="MHELSLVESLVTALEERARQEGWGRIQGVRLRLGALRQVVPEVL
                     EFCFQGAIPGTPLAGAALELEEVPALFRCVGCGGEVPLGGRCPACGGPGGEMISGLEW
                     EILSVDVEEEG"
     gene            4678..5361
                     /gene="hypB"
                     /locus_tag="PL162_00665"
     CDS             4678..5361
                     /gene="hypB"
                     /locus_tag="PL162_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HQF90573.1"
                     /translation="MTRRLEIRQSVFARDERVAGEIRREMASRGILFLNLIGSPGAGK
                     TTLLEHTLGRTSLRCAVVEGDVATDRDAARIAARKIPVVQINTQGGCHLEAHLVRRGL
                     EALPLDGVDLVVVENVGNLVCPAEFDLGEDAKVAVASTAEGDDKPLKYPSLFVQARGV
                     VLTKTDLLPHVPFSRERFWGDVALLKEGTERFEVAAPRGEGMEAWIAALERWAAEKRR
                     PKAPGGAPQ"
     gene            5358..7367
                     /locus_tag="PL162_00670"
     CDS             5358..7367
                     /locus_tag="PL162_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HQF90574.1"
                     /translation="MMGEGGGVAGPSSRRESKLLEGLNPVQREAVCHLEGPLLVLAGA
                     GSGKTRVLAHKVAYLVEQGVSPGRILAVTFTNKAAREMRDRIEGLLGGSAGEMAISTF
                     HSFGLRFLQRHGDALEARGWPRRFTIFDRQDSRALVKDLLAELEIDPQRLDPSGALER
                     LSSAKGEASVRTLEAPAMDPALNRLFRRYQECLREQGALDFDDLLLLPLHLLTVEEEV
                     RQREQRAFDWVLVDEYQDVNRLQYLTLRKLVEGKEQLVAVGDPDQSIYGWRGADMGMI
                     LRFSQDFPRSRTVVLDQNYRSSGVILDAANALIDRNAERPKKNLWTARDRGEPVRVLL
                     VESERDEASFIASEIEALRGQGYRYGDMAILYRLNALSRGIEQLLFERRIPYRVVRGT
                     AFYDRREIKDVISFLRLAINPRDGAALGRCANLPPRGIGARGVERLGAWLAAEGGNRA
                     EDVWRRLAEEGAGLTGKGRQGARELGAAMEALGQRRDDVPGAVDYVLNEMGYEAFLQA
                     EEGDSFEERRENIQELLSLVPDGASLDSVLGEIALYTDAELASDKEDAVGLLTLHAAK
                     GLEFPVVFLAALEESIFPHARCVEMPGGIEEERRLCYVGMTRAEERLYLSGARSRLLF
                     GTVQRHPFSRFLRELPPGPVALEDRTGRKESFHAGFGSHRGYRRW"
     gene            7327..8217
                     /gene="coaE"
                     /locus_tag="PL162_00675"
     CDS             7327..8217
                     /gene="coaE"
                     /locus_tag="PL162_00675"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HQF90575.1"
                     /translation="MLVLALTGDIGAGKSTLGALWREEGAQVWDADILAREAWELPEL
                     RAQLRERWGDAIFSPGGPLDRRALGERLFADEESYRWGCDRVHPPVIKALEERVRESR
                     RGWVVVEIPLLFEVGVPSWVDGVVYVGAPERRREEWNAHRRLAGADLAQRQRWLLPEG
                     VKRSRAQVVLENDGDMESFVAEGRRLGHRFRRMASAVELRGVWAEEAEARRLGRELVE
                     ARLGARVSVAEEGRILRRKGTVVEERGWAVTVTTLEELVGAAMERWGLSSSGAPDAVT
                     VLPRGRSPWEELVEIEENCR"
     gene            8214..10874
                     /locus_tag="PL162_00680"
     CDS             8214..10874
                     /locus_tag="PL162_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQF90576.1"
                     /translation="MKVVTSHVGCDFDALASMVAAAKLHEGSIPCFSGAAGRNVREYL
                     RRFDFWKIRTPGKINMDAITTLVAVDVRSRNRIGPFREVAERPGVDVVVYDHHPPVSD
                     PLVAREEYIEPVGATVTLMVELLTSRGIPISPAEATLFSLGIYEDTGMFTFAGATRRD
                     FAAAALLRERGADGPLVGSFVDFSLNSEERGLMEALVAGARERYIHGARVVLASARCA
                     GYVEGVSLFVHRIRDAFDADVAVAVVSMGQRMYVVVRSREHVLDAAQFLEPLGGGGHA
                     QAASASLAGDDVDHTLRSLEKQLEHLLESVLRVRDVMSSPVLAVSPATTVEEAVRLML
                     RYGHSALPLVRDDALVGVVTRKDLDKALLHGFGVAAVGEFMTENPWVVFPDAPVAEAH
                     RLLVQGNIGRLPVLERGGLVGIITRTDVLRALYPTSLMGSEQPPVRGRVWEEDVGPLL
                     QRHLSKEAYDLLERLGRRGAEMRLRVYAVGGFVRDLLLGAPGLDLDVVVEGDAVAFLE
                     SWRGDGCRVAVHPRFATGTVIFPDGQKVDVATARREFYEYPSAQPRVASDSLKHDLYR
                     RDYSVNAMAVALNPEIWGTLVDYFGGRRDLRYHTLRVLHNLSFVEDPTRVFRGVRLEQ
                     RLGFRLEDTTLRLLKTCVQSGMLGMLSGVRLRSEVELLLQEEHPLKAVRRLTSLGAWE
                     MVFSGFSFGLRGSFAMRRLSALWRLTKEDLPDFGRDRWLIFLGALLEECSPAGRRNVP
                     DRLSLTPGERGVLGRIMTGREEAERRLGGSGDLPPSALWKAWHREPPAALFFWGATSG
                     NWRFRRRVLLYLTRWIGVRPMLVGQDLLDGGVPKGPAVGAFLEGLIMARLDGGVETRE
                     DELEWVEREKARLDRGEAPRQKKGGQKKLG"
     gene            10963..11571
                     /locus_tag="PL162_00685"
     CDS             10963..11571
                     /locus_tag="PL162_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HQF90577.1"
                     /translation="MLGLPSLGELLLSLPAVLWAITFHEFCHGYVAYRLGDPTAERQG
                     RLTLNPLAHFDPLGALMLLLFRFGWAKPVPIDTRYFRNPRRDLILVSLAGAGGNILTA
                     VVAGKLLQWFPGVFGANQALFDFMIILIFMNLGLAVFNLLPIPPLDGSRVLYVLLPPR
                     MLEFYFRLEQYGLWILLILVMFNVLPAITTPMVRAMVTLIFA"
     gene            11582..12361
                     /gene="surE"
                     /locus_tag="PL162_00690"
     CDS             11582..12361
                     /gene="surE"
                     /locus_tag="PL162_00690"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HQF90578.1"
                     /translation="MKLLITNDDGVFAPGIAALADTLVDRGRDLVVVAPDRERSSVGH
                     AITLSRPLRLWPVISGLYERTHRVFAADGTPSDCVVLGLEEVAPDATLVISGINRGPN
                     LGDDLTYSGTVSAAMEGVILGRPALAVSLGIAPGDGTHHYDTAAAVTARLVDRLESLD
                     LPRGVLLNVNVPNVPYGELRGVVPTRKGVRLYLDKVSRRTDPRGNTYYWMSGRPEDEL
                     TEGTDVWAVASGYVSVTPVHMDLTHYPTLQELDRVVATHPL"
     gene            12503..13987
                     /locus_tag="PL162_00695"
     CDS             12503..13987
                     /locus_tag="PL162_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HQF90579.1"
                     /translation="MKRFLSILFLGLCLWGGGVSGALGALSGDRFMDLCRSGTPKEVR
                     SALKKGAPVDFCHREGWTPLMVAAAKNPSPQVISLLIKAGAEVDARNKDGWTALMLAA
                     AMNPNAAVAGALLDGGAAVDARNREGWTPLMVAAARNGNSQVVLALLKKKADVNARNK
                     DGWTPLMAAAAKTSRGEIVAALLKAGAEVDARNKDGWTALMLAAAMNAHPEISETLIE
                     AGAEVGLRDPRGITPLMAAATSNANPRVIARLLEAGAWRGASEEGEWTPLMAAAWHNP
                     NPDVIAALLRGGLCVDARDQEGATPLMMAARESSPGVVEALLRGSPDVNARDQEGRTP
                     LVFAAQGASQDRTIRLLVGAGGDVHSRSALGWTPLMYAAAMNPNPSVTVSLIEAGADP
                     NATSGEGFPPLLFAVLENPNPQVVAALIRGGADPRARFPHGRTLLMMAALKGSHPEAL
                     LELLRGGADPQARDQEGRRALEYARENPRLRDTPAYWKLNDLSY"
     misc_binding    14182..14418
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            14465..15706
                     /locus_tag="PL162_00700"
     CDS             14465..15706
                     /locus_tag="PL162_00700"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HQF90580.1"
                     /translation="MYKGKVVLAFSGGLDTSVAIKWLQDQGYDVVTYTADVGQRVVDL
                     DGIQEKALKTGACTAHVQNLRQEFVEDFVWPALKAQALYQGTYPLTSALSRPLIAKHL
                     VEVAQAEGAVAIAHGCTGKGQDQVRFEVCAGALDPSLKVIAPVRDWHFSREAEVAYAE
                     EHGIPVLQHVKGIYSIDENLWGRAIECGPLEDPWNEPPSDAFQLTVDPWNAPDEPLYV
                     EIGFEKGVPVSLNGKPMDGVTLIQTLNTLAGGYGVGRIDMIEDRLVGFKSREVYECPA
                     AAVLMQAHRVMESLTLSKDVLQGKESLSQAYAKMTYEGYWYAPYKEALDAFFDHIQQY
                     VNGTVRMRFFKGQAVAVGVKSPNALYREDLATYSEADTFDHSAAVGFISIWGMPIKTW
                     REAHPHEAPLEMIPLRAVGEE"
     gene            complement(15799..17538)
                     /locus_tag="PL162_00705"
     CDS             complement(15799..17538)
                     /locus_tag="PL162_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HQF90581.1"
                     /translation="MYSEALRRYLPAFGRPEEPFLRTVSPAPRESVRRWTRGEFWLMA
                     LRAAGVLRRFGLGKGDRFVNAYGENTPEDLMFRLGATMVGAVPVTMNWQADGVEEMAY
                     KIDLTEGRLMVWDPSFLALKAPGGADFLKELSRRFPRLPDYAVSSLAEESPLEEGAFV
                     ADLTSADDRIVIFTSGTTGHPKGARLSYGSYDNNDQALGRYLELPEDPPEQGPLFLMI
                     NPLHHANSTAVSDLVLRRPGAELVLFPRYGTFYWRAVTECAEGEGRSRRIIAPTVARH
                     FDYLEHLRETASLPVEEPRLLQSMARVEFVLGSAPVGPTTVERLERWTGRVPSVRFGS
                     TETCFHVLGTPLAMANDARRRAFERGWRHSPPGYWIGRPHPPLTEVRIVEEVDPDKEG
                     YLRDCTEGRPGYMICRGGNVMTGYVRDEEGSRRVLREGWYLGLLDVAFGLTHPQDGQR
                     DIYWVGRDSALLIRGGANYACDQINGELCRFVERRYALSCDDFDLAVAGLRIASEHED
                     SCGVTLELKSDRARSLEEELRRTFLVEAREEVSKGARPDFLRFGQVFRTFKGTIRLQE
                     IKKAWRDDPDAPQ"
     gene            complement(17670..18824)
                     /locus_tag="PL162_00710"
     CDS             complement(17670..18824)
                     /locus_tag="PL162_00710"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HQF90582.1"
                     /translation="MERVRFIPIAQLADCGGTVAEDIVSPTKALLLPKGTELRWIQTS
                     LPSVVRRLSEAGIGRVAVQDSLETSMESLEEVLQTLSPPQLSQVNHSLARHATRQIGD
                     VYSRIENHQVTPEDLGHLMDTGKTLAREIARTTQISLSLGRVKEQDEYTFVHSLNVAL
                     LSGFLAHRILGDPAAVESITVGALLHDLGKALIPREVLNKPGKLNDQEFAVIQRHPEE
                     GHSMAVQNGVVDEVALAVVRFHHERWRGDGYPHKLNGEEIPLPARISAVADVFDALTT
                     ARVYKGPIARDQAISMIVTSGGSHFDPAVVRTMLLALGLYPPGMLVELADGSVGTVVA
                     AKEGDLMRPLVLLQVDGRGFSAPPATVVDTSTSPSLFIRRSLGDMEKRSL"
     gene            18915..19604
                     /locus_tag="PL162_00715"
     CDS             18915..19604
                     /locus_tag="PL162_00715"
                     /inference="COORDINATES: protein motif:HMM:NF015181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HQF90583.1"
                     /translation="MFSKKEENGMILAVSGLKNTGKSTLCRFLVRYLGDRGLRVGYVK
                     HSDHALFREEGTDTGKILSQEGIAVHWGADGFRLERRGDLSPQDLVRECFPGGDLVLL
                     EGGKALPLPRIWVGDPDDLPEGTSGVVAWYPGSSKKMETPGDNKGVSCFDRPEALGDF
                     VEGLVRKSARSARVFVGEKPLPLKDFVADFLRGSIVGMLRSLKGVGDPEKEGILVAFP
                     PERPLGDKRPL"
     gene            19777..21222
                     /locus_tag="PL162_00720"
     CDS             19777..21222
                     /locus_tag="PL162_00720"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HQF90584.1"
                     /translation="MLATTAIILMFVVFLALISRKLNVPLILIALAIGIFFGSDVTGL
                     IYFDDASLTQRVANGALIFILFAGGFGTKEDHLRPVFEITMLLATGGVLLTAAAALLP
                     FVLITGFEVKTALLLCVIISSTDAAAIFSILRTRSINRRLTSITEIESAANDPMAIIS
                     TVFVIQLITGAHVGTLETVTSFLWQLLGGVGLGVLFGLGGVFVFKKIRDIDLGYFYLF
                     LIALIILTHEVANFCQASGMLAVFFAGLTMGNKSFPYKYGISSFTEALSFVANVLLFV
                     LLGLLVFPKDFPAIWMPAVGLFVAITFVGRPVAILVCTGLAKLSLKDKAFLSWSGIKG
                     AVPIVLATYPAAAGLDPNHHMFNIVFLAVLCSILTQGTTIGRLADALGLAVKGQKKSR
                     QSMELITAHETSYELIEVYIDDDLYQGECRIMDLSLPVGTTITMINRDDVIIAPAGKS
                     RLLPGDILSLLVDIGNVDSAVGEILSKFGKK"
     gene            21319..21394
                     /locus_tag="PL162_00725"
     tRNA            21319..21394
                     /locus_tag="PL162_00725"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21352..21354,aa:Lys,seq:ttt)
     gene            21399..21474
                     /locus_tag="PL162_00730"
     tRNA            21399..21474
                     /locus_tag="PL162_00730"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21433..21435,aa:Glu,seq:ttc)
     gene            21479..21554
                     /locus_tag="PL162_00735"
     tRNA            21479..21554
                     /locus_tag="PL162_00735"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21512..21514,aa:Thr,seq:cgt)
     gene            21981..22547
                     /locus_tag="PL162_00740"
     CDS             21981..22547
                     /locus_tag="PL162_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQF90585.1"
                     /translation="MFSPQDSEGEGDRLLWRRCKTGDEGARESLILKYRPLVFWFVRQ
                     FQVSRSSFPDLVQEGMMALIEAVDRFDPEKGTRFSTYAYYRVRGHMVNFLQRRESRAP
                     LPVEDVEPFMEQNDDSMSPDWALALRDGMASLSDRESAVIDELVVQGTRAKDYAQELR
                     VDVSYVYRVQRRALAKLRLWMGLEGSTR"
     gene            22666..23301
                     /locus_tag="PL162_00745"
     CDS             22666..23301
                     /locus_tag="PL162_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90586.1"
                     /translation="MERKIRRVQRWLDRCLEACRIGQWQAALAEMECARTDLDLARRA
                     LWDELSAVDKSTRRRPFRGGAALRGALVGALVILAVAHPLAFDDPSGAPSPSLVPEIV
                     AFVTRDEQSLILALRTRLGEGALRQGAGEWSQLSSEEEGALRRDAGARAAGRGVPPER
                     KSPSGAGRSAVLKEDLPRATEGGGLPAEDLLALVQVGERALRDASVRVVGD"
     gene            23353..25107
                     /locus_tag="PL162_00750"
     CDS             23353..25107
                     /locus_tag="PL162_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HQF90587.1"
                     /translation="MKRLMAFLLWGALLGWGGAAWAAEPLVVSLSVEGNSQVVAAHIL
                     SAVDTKVGEPLDRERLKNDVEAIYELGFFSVVDSRLSEQSDGVAVTFLVHENPVIEDI
                     RFEGNTVYSAEALRSVLFTAPGNVFNRVFFQHDLDRIKEKYQKDGYVMMRVLDVQVDG
                     GVITVKLVEPRVGDIIIQGNTKTKTHVIRRELRVKKGDLFNATLLRFSINRLQGLNYF
                     EDVSVGFEPGESDGEVNLILTVQEQKTGRMTFSIGHGSASGWSGGLGYDDSNWKGLGH
                     KVSVGFETGKKEQYWINYTEPYMDDTHYAWKAGFYKRTWEDLMRYASSDQGDDVESVK
                     YDQKKTGVYYGFGKKFRGNSKMSWFVTLDWHDTEVIPKSGDFDPYYSRNNLLSGKIFT
                     VQASLTRNNLDEYTPYPKGDLEVLTVDQALETLGGEYSFTKYSLEARWYLPIRGLARY
                     VDLFDKVDEEENPIIFATRMRAGFSSGALPWAEQYFVGGGTTVRGFKDDRYEGDEMFL
                     ANVELRVPFEKNFSFVVFYDTGMAWDKDLGESFSFSELKGAYGIGVRVKTPLGNIRVD
                     VANGDNETETQFGFGELF"
     gene            25178..26365
                     /locus_tag="PL162_00755"
     CDS             25178..26365
                     /locus_tag="PL162_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90588.1"
                     /translation="MVPQESFGAVRVIGVPLWLRSTAERSLEAVWQELRSRPGSPQEH
                     LEILGLVAGRLFAGYGVKSASGEGDPVVVLIPAVAPPRWSLRLEGPALSGPPGEWLSS
                     DVRRTEKALAPLLDEVPVEALGWGDGALRKVLQETGEQELPGWELAPLVQLSGGQAEL
                     RVRLLPSPPLVLALAPSLRSTSLPLMLKKDLTEALLKNLAPIHGLPVAWVRHHQEEAE
                     RWAGDILEDRNTVQNTRASVAVRMKPKEIAPVDVRVESRTYVLQAWMAAYAGSDALYP
                     EVGIHLGRRASLWSRGEVELYGEWIAKVHDFGLESRWGVRTSPVGDLWLGVEVVWPGE
                     TVWGRLWLENDARRAYAWWRLSRDGDNQGSVGWHLTDHISLELHYDDRVPDQLILRAV
                     GTL"
     gene            26430..27458
                     /locus_tag="PL162_00760"
     CDS             26430..27458
                     /locus_tag="PL162_00760"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HQF90589.1"
                     /translation="MKAKKPFSLGDAARMTQGAVRGDVDRQFRGVCSPGRLREDFLVA
                     VWDRRCLEDVPPSWGVMTFPSWAPEGQDAVEVEDPRHALVLLLQGVYEAPPRAGGIHP
                     SAVVSPEAQVSPQAWVGPGCVVEEGAVVEEGAMLEGQIFVGRDVHIGSQVWIAPQVVL
                     YPRTTVGARTRIHSGTVLGADGFGVLLGDEGAREAIPHVGGVLIEEDVVLGAHVTVDR
                     GTLDVTRVGAGTKVDDHVHVGHNADIGRRCVLVAYVGIAGSAVVEDGSILAARSGVAP
                     HVRIGQGAQLAAMAGATRDVPPGGVVSGFPAWDHKKALRVESLMRRLPELFERLKRLE
                     KGRSGESS"
     gene            27455..28288
                     /gene="lpxC"
                     /locus_tag="PL162_00765"
     CDS             27455..28288
                     /gene="lpxC"
                     /locus_tag="PL162_00765"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:NF015299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HQF90590.1"
                     /translation="MSRTVARPVTFFGRGLHTGESCRVTVHPSSEGGVRFRLSGNSFP
                     VEEARFSGDGRGTTLAFPRGGSVATVEHLMGTLSGLGIWHATVEVEGPEIPVEDGSAA
                     PLAALILEAGTGPAAPVIPWAVPFPVAVDDPDRGRFAVALPGEGCKISYVLDYASPFL
                     GTQIQDLEWTPESFCRQIAPARTFALLEEVEELRRRGLVRGGSLENSVVVGAEGVMNA
                     GGWRFPREGVCHKVLDLMGDLALVGRPLEGRFLVCRGGHALHLALLRRLRSLGAGGPK
                     R"
     gene            28437..28889
                     /gene="fabZ"
                     /locus_tag="PL162_00770"
     CDS             28437..28889
                     /gene="fabZ"
                     /locus_tag="PL162_00770"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HQF90591.1"
                     /translation="MINIQEIFRLLPHRYPFLLVDRILEVDGEHVIGLKNITINEPFF
                     VGHFPDEPVMPGVLLLEAMGQVAAMLVVNLPEASGMVSYLTGVNNARFRRPVKPGDQL
                     ITEARLIKFRSRVGKVQVTGRVDGEVAAEAELSFVLAQGLSREDSKES"
     gene            28987..29799
                     /gene="lpxA"
                     /locus_tag="PL162_00775"
     CDS             28987..29799
                     /gene="lpxA"
                     /locus_tag="PL162_00775"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HQF90592.1"
                     /translation="MAPQIHATALVAPGAEIAADVRIGPYCVVDGRVRIGKGSVLHAF
                     VRLCDFVEVGEGCELCEHVTLGREPQDFGFRHEESWVRIGSGVVLRENVTVHRATGEG
                     NATRVGDGAYLMEGVHVGHNVQVGPGVVMASKAGIAGYVEIGEKTVVGGMVGIHQFVK
                     VGRYCMLGGASKIVRDVPPFALVDGRPARMFGINKVGLRRHGFGGEARRHVLSLYQSL
                     YQSGLPLRQALEEIKANLAPEDALGREIVAFAEGAHRGLVPWGRRHDGGDHD"
     gene            29792..30265
                     /locus_tag="PL162_00780"
     CDS             29792..30265
                     /locus_tag="PL162_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HQF90593.1"
                     /translation="MIKLFAMDVDGTLTDGGIILHGSGGESKRFDVQDGLGIVRLQAA
                     GVEVAFVSGRRSPATLRRAEDLGVRVCRQGVKDKLAALQDLAKDRGLRPSEVAFVGDD
                     LNDVACLRWAGWGVAVANAIPEAKEAADMVTRASGGAGAVREAAEFLLKRLMAGD"
     gene            30269..31372
                     /locus_tag="PL162_00785"
     CDS             30269..31372
                     /locus_tag="PL162_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HQF90594.1"
                     /translation="MGIFSGKILDRYVLGEMGQPFCFGMMAFAVLFVAGGLLFEIADL
                     IIERGVSFYVVLRLFLYRLPSVVVVVVPMAALLATLLSFGRLSSQSELVALKAAGVSF
                     QRVLRPVLWGALGVTFATILFNETVVPLSNAATDQLMRVEVYRQRPSLIKERVFLREE
                     HDGQLSRVIYIDKLKPRVGRMEKILVQEFDKGRLSRILSSGGGVWADGVWTLEGGQVF
                     EVTPAGDVALLMTFERQKIPLDLSPEEIERSGRDPDTMGVFEILRQISLVSLQGGDVH
                     PLWISFHTRLAFPWATVVLALVGATLGVRPHRSGAGIGVGLSVLVAFAYYVVMSFSRA
                     LAQGGNIPVVAGAWLPVAVFLVLGGFFARRANG"
     gene            31411..32274
                     /gene="lpxI"
                     /locus_tag="PL162_00790"
     CDS             31411..32274
                     /gene="lpxI"
                     /locus_tag="PL162_00790"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162707.1"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HQF90595.1"
                     /translation="MMTARSEETGAKAPLLERWALVAGGGALPEEIARRWALQGRRGV
                     IYVLGGRAEVLRPWGEATVELDRADLRGTVEDMLRRGVRKVVLAGTVPKNTMYRPSQM
                     DGTLQELLRRLPDSNDHALLGAVVAALEGAGMTVMRYGDVLGDLMAEEGILGGREPSP
                     EEWGDVAYGVEVASRLVPLSFGQTVVVRRRAVVAVESMEGTDGTLERAGRLASGGVVV
                     KLMRGDQDDRYDLPVVGPRTVRLMGRWGLGCLAVEKGRTIILDREKVMALAEAEGIAV
                     VGVAPRRIPEA"
     gene            32302..33435
                     /locus_tag="PL162_00795"
     CDS             32302..33435
                     /locus_tag="PL162_00795"
                     /inference="COORDINATES: protein motif:HMM:NF014714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HQF90596.1"
                     /translation="MVEGMRSFVLSCGEASGDAYVGLLARELRAQGFAGELWGMCGPR
                     GASAGVTPRWSFQELQLMGISEVIPALPRLFRLREEMIRAICEARPGGVVMVDSPDFH
                     LPLARGLRRRGYEGKLFSLAPPTAWAWRPGRVKTLELFSLVFPLFPFEDRFFRSHGVR
                     SAFRGHPLLDEPSLAPSDRRDPPRRVALLPGSRPSEVRRLGEVLGGVGRRLKELGMEP
                     ELSLAPSLEAPDREFLRRAAEGLSLSEDPGPELMRRADLVVGACGTAAVEALLLRRFM
                     VVLYRGSWSSHLAYRLLVRTPFVALPNLLLDRPIYPELLQGEVREERIVKEVLRYVED
                     LSFRSSVDQELRRGRERLGDPGALAFWARAVREELTSSEGVGA"
     gene            33432..34565
                     /locus_tag="PL162_00800"
     CDS             33432..34565
                     /locus_tag="PL162_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cdisaccharide synthetase"
                     /protein_id="HQF90597.1"
                     /translation="MIPPASSPVLLLVNGPGELWGWGRPLAAALRRRGLEVRLRLLPC
                     QFAGGEERLLLERLPSVRGEGPEGYAAFFLRGGTPAAAVVQLGGELGVGRRVAERWKA
                     PLVCYTYGFKRGLERCDLVATAFNPMAAGLNGVRCEVVGDLVADALTLDRGPSPWGEA
                     PSRGKNHCRLALFPGSRPAIRREARGFLQRLVETLRREGGEFFPVAVLSPAVDDVEAE
                     QWREGGMLPWRGGAGTVLPGAHMALTQPGTNTLELLHCTTPGLVVVPDEFFDKIPLGG
                     VAGLAKSLPGLGSFLRRRALDRWRGKFLSWPNRLAGGEILWEAVGAVTPESLARRVRE
                     LWTDEVGREKMTRSLRELAEEIGPGAGAADRLAERIGEMAAGR"
     gene            34565..36313
                     /locus_tag="PL162_00805"
     CDS             34565..36313
                     /locus_tag="PL162_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQF90598.1"
                     /translation="MGKAGGSYRRLLAHAWPYRRKVYGAFGAMLGAAICGMIPPWLLK
                     DVVDDVLIGRELGMLNLLAGGIVGLYLGKAAFSYAQRYLMTEVGQRLVLDLRIRLFDH
                     LQRLSLAFIAERRVGDLLSRITNDVAVLQEVATTALVDLVVQGLSFLGMAGFLLYINW
                     RLTLVTWTILPLAAWFIDRSSKRLRGVGHEIQSCLASLAAGAQEALGSLRIVQAFVTE
                     EEELDKFRQRSEANFRALMRGTHILALLEGGVEVALVAALAFILWLGGRDVIEGRLSP
                     GELIAFLGYLGFMVQPLRVVSRSVSRISQGLASADRIFDMLDQPAGLVLPASPLSPGR
                     LRGEISLEDVHFAYDPARPVLRGLSLGIVPGEVVALVGATGAGKSTLADLVPRFYDPQ
                     RGVVRVDGHDLRTLNLPELRRQIGIVPQDPVLLKGTLFYNIAYGCAWADEGRVRDAAC
                     QAGIADFIETLPRGYDTEVGERGATLSGGQRQRVAIARALVRDPRILIMDEATASLDA
                     DVEAQIQAALRRVFRGRTVLVIAHRLSTVRDAHRILVLDQGRVTEQGTHEELLARRGV
                     YHRLYSLQFGAAGGGK"
     gene            36320..38737
                     /gene="lpxK"
                     /locus_tag="PL162_00810"
     CDS             36320..38737
                     /gene="lpxK"
                     /locus_tag="PL162_00810"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HQF90599.1"
                     /translation="MSERKAERVLQSFLAHARGEEAFVGWHLLAPLGWAVGAWARWRN
                     RGFDHGWWLSEEPLLPVVSVGNLTLGGTNKTPFVAMLVETLVRAGLAPGVVSRGYGGR
                     TRTPLLVEGGRGDRALTGDEPLLLSAQLRPCPVCVAADRRQGVALLAQRGCDVVVADD
                     AFQHRRMGRDVDVVLVDAACPWGNGRYFPAGLLREGVEGLVRSHAVVLTKVDQVLPEV
                     LEDLERRLKSIVPAERIFRSRLAFGGWFRARDGGWEPDGPDFSGGVLAFSAIGSPESF
                     RRLLERQGVRLRGERIFRDHHHYSPEDVMALERLARTLGVRSLVCTEKDVYNLPLSPG
                     GSPFCPDDKPPPQEDLSVWFPRVRTVLDDPGRFWKTLGQGLVPRMVVASNGFGEDAMG
                     SLLADKLRRRFPGGRVEAFSLVGRGQAYAERKIPVLSPPAETPSGGVVKYSWGDFWRD
                     LRSGLLGQVRGQLRAWEDYRGRARTVLCVGDVYLLLHTLWGQGQAPLLVATAKSELLG
                     GHWHLERLVLRHRCRMVWTRDGATAEELRRHGVPAAFCGNPIMDLAEEDIQKIPLSGD
                     NIRGPGGKGAPPRVLLLPGSRGRAYDDLALLLDAAVKIWERQAVSFLLVLAPTLERGR
                     FAEAALRRGWRLEGETLLGPSGLRVRVTADPVARGAKGAQVVLGLGGTANQVCAGLGV
                     PVVSVLEKGKLVQKKLLGEAEVLVPPEADALAQEVDALLRDEARRCAMGRAGARALGG
                     GGALDAVVSHVASVLGWEGRCAVAERLNAFLASWGPETPGASGGGWGAPEGPAGTVPG
                     DAKEMKI"
     gene            38734..39483
                     /gene="kdsB"
                     /locus_tag="PL162_00815"
     CDS             38734..39483
                     /gene="kdsB"
                     /locus_tag="PL162_00815"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HQF90600.1"
                     /translation="MSTLAVIPARYASTRLPGKPLVPVAGVPLVVRVLRNVQRCRRVD
                     AVVVATDSPEVRLVVEAEGGVVEMTPPELPSGGDRVAWVARRRSEDVVLNIQVDDPLV
                     GPDMVDPLVEALEADEAIQLAVLAKRIERIEEVPDPHIVKMVFDGQGKALYFSRSPIP
                     YPRHPGGVWFKHIGPYAYRRNFLLDFASWEPTPLERIESLEMLRVLERGHAIRCVPVE
                     RDTIEIDTPADVEACERHLARLAEEGDHHGA"
     gene            39473..40321
                     /gene="kdsA"
                     /locus_tag="PL162_00820"
     CDS             39473..40321
                     /gene="kdsA"
                     /locus_tag="PL162_00820"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HQF90601.1"
                     /translation="MERSVRVGDVTVGEGTLAIFAGPCVLESLEMALEVGEACRDLCR
                     SLGLGYVFKASFDKANRTSLSSFRGPGLERGIDWLREIRDRLEVPVLTDIHEAWQAGP
                     VAESVDLLQIPAFLCRQTDLLLAAAQTGKPVNVKKAQFLAPQDMAPVVEKLRGGGCRE
                     ILLCERGTSFGYHQLVVDMRSLPIMRDLGCPVVFDATHSVQMPGGQGDCSGGDRRFVL
                     PLARCAVAAGVDALFMETHPCPEKALSDGPNVVPLNRMERVLSEVAALFRAVQPLGPA
                     DLAWWQ"
     gene            40330..41331
                     /locus_tag="PL162_00825"
     CDS             40330..41331
                     /locus_tag="PL162_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HQF90602.1"
                     /translation="MLPRDRDLCPVEGEALLETGRRVLRQEGEELLRAAGRVGDEMVE
                     AARRIAACDGRLVVSGLGKSGLIGRKIAATFASLGTPSFFLHATEGSHGDLGMVCRGD
                     VGLFLSNSGETREVLELLPFFRRIGAPIIAMTGKESSRLAQEADVVLLAAVEREADPL
                     NLAPTSSTTLQLALGDALAGMVTELRGLCQEDFALFHPGGALGRKLLLRVEDLMGSGE
                     RLPRVERGATVSEALFEMTSKGYGATAVTDEEGFLVGIFTDGDLRRFLERSLHPSMDA
                     PIEEAMTLSPKTIAPDRLAAEAVLLMERHEVSVLMAVRQGKPVGIIHLHELLKAGLA"
     gene            41333..42610
                     /locus_tag="PL162_00830"
     CDS             41333..42610
                     /locus_tag="PL162_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HQF90603.1"
                     /translation="MVEGRGLGEPWWLGPYGLLARGVHAAWEPRLRRRYGEGYDERRG
                     ELGKDLRELGKAHPLWIHAVSVGEVQAAAPLVRIVKEESQGGIPVVLSTITPTGRAMA
                     RTLLEGYLDALIYYPWDVPTFVERAVEALSPRGYVVMETEIWPTLLFALRRRRIPTFL
                     ANGRLSPRTFDRARRWSFVWKDVWGSFEGLFMRSEEDRARIVALGAPGERVQVLGDCK
                     VDALLHRRGAPETLSVAPPCDGPFVLAGSTHAGEETVALEAFRQLRGECPSLRLILVP
                     RHPERGREVCQEAQLTGPAGLFSENPGSWTILVVDRVGVLFSLYRWARGAFVGGSLVP
                     RGGQNLLEPAAWGVPVVRGPHMEDFAEGAKALEFLGAARTVTDASSLAEAWRWTLSDE
                     GRDAGLRGRDYVEGLGGAARATWRAMSPRWEKM"
     gene            42674..43840
                     /locus_tag="PL162_00835"
     CDS             42674..43840
                     /locus_tag="PL162_00835"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HQF90604.1"
                     /translation="MFLAGRTQFGPGVVSKVGELAKSLRAERAVLVTDGSMERLGVAD
                     RVEKLIHEAGVECCVFSGVEAEPSVETTDAVAELAREHDCQLVVGLGGGSCLDVAKAV
                     SVLITNMGSAAQYQGMNLVKERGVPKILIPTTAGTGSEVTFTAVLIRKSDGFKGGIND
                     EKLFSDYNLLDPELTLSMPPEVTAATGMDALTHALEAYTGRAASVFSDIFAEEALRRI
                     GRYLRVATWNGKDLHARSEMLLASYFGGVALANAGVGACHSLAYPLGSVFGVGHGMAN
                     ALLVPYVTAHNALAAPEKYAQAAALMGFGGDGLSLRDQAFICADGLEELVEDLGLPRT
                     FKDLKRRKIIKEEISVDLLPELATKAMAVARPMENNPRFMTEESCRNLYREAME"
     gene            43840..45048
                     /locus_tag="PL162_00840"
     CDS             43840..45048
                     /locus_tag="PL162_00840"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQF90605.1"
                     /translation="MPGFELFDQQEIDALADVIRRKMVHRYSFVNCRDEIYRVEEFEN
                     AVAAKFGVKRCQAVSSGTASLYVALKAMGVGPGDEIITTPFTFIASVEAILECGATPV
                     LAEIDESLNLDPSVLEEYITDRTRGIMPVHMFGAAADLDRIQEVCTEYGVPLLEDSCQ
                     AMGATYRGKALGTMGLWGTYSLDPYKILTVGEGGLVVTNNEELYRRMEYYADHGHIHS
                     KTIDRGAEEKACLGFNFRLSELQGALGLVQLAKFDDAVARLRSNKKRILERVGNVEGL
                     SLRDLPDRSGEIATQLVFLLPDAARAKVFQKVSKEAGVGCALLSENTWHYARHWQTLR
                     DGASWARVRCPFDCPHAPETPPLYRPVEWPKTHGILSRALCYGIDICMPDERIEKTVA
                     AIRAAARAAL"
     gene            45191..46369
                     /locus_tag="PL162_00845"
     CDS             45191..46369
                     /locus_tag="PL162_00845"
                     /inference="COORDINATES: protein motif:HMM:NF018017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HQF90606.1"
                     /translation="MSESSIQGVGVLSDGLRGHLSQSRGVAEWLRRDFGVEGKEFDVP
                     RLVGWRRWQALKLRALTLRRGNPATALRWLAASSGIPLLESVKIWAQDQGMDLRRILF
                     LSAGSAAAPFCLALARHLGARCVTLMTPSVLGTAPFDAAVVPRHDAPVPSPWTLVTLG
                     APNAIVPQALAEAGEELLRRFPAGGPEGEGEVWERWAVLLGGDDGNYRLSPRWMERSV
                     GALLSAAKARGAVLYITTSRRTSTEAEEALLRSVASSSAVRLCLLASRDPWNPVPGLL
                     GAAHRVFCTEDSVSMVSEALSGGHRLGLLRVERRGGLRGWWGHAGTKLVRWGVWGEGA
                     LGGAIRFDALFEEFFRRGYAVECGPDPEAWPWDRVGRFDTPPPNEARRAAAWLMGLWP
                     "
     gene            46614..49100
                     /gene="leuS"
                     /locus_tag="PL162_00850"
     CDS             46614..49100
                     /gene="leuS"
                     /locus_tag="PL162_00850"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HQF90607.1"
                     /translation="MGYDFSAVEAKWQSYWERKGVFHTEAHEEKPKFYCLEMFPYPSG
                     ALHMGHLRNYSIGDLLSRYLFMNGYNVLHPMGFDAFGLPAENAAIKFGVHPNAWTWKN
                     IEHMTDQLKHMGCSYDWRRRVETCHPDYYRWTQWLFLQFYKKGLVYRKHAQVNWCPDC
                     ATVLANEQVIDGGHCWRCHSLVTKRGLEQWFFRITAYAQELLESLDRLPGWPDRVRIM
                     QAHWIGRSEGVRLSFTLKGTEERIETFTTRFDTIYGVTFLALAPEHPLVERLAATSPE
                     GDRIREFVQSVISASEIERTALGGEKKGLFTGHYAINPVNGEQVPIWIANYILMDYGT
                     GAIMGVPAHDQRDFEFARKFHIPVRVVIAPADGSSLDGASMDRAFDENGIQVSSGDFD
                     GLPTAEAIPRMTAWAEEQGLGTREVNYRLRDWLISRQRFWGAPIPMVYCDRCGVVPIP
                     EEELPVVLPTDVKLLDGGRSPLPEADHWKNTRCPSCGGAAVRETDTMDTFICSSWYFL
                     RYTSPWTKQAPFLKEDVDYWMDVDQYIGGIEHACLHLIYARFFTKALADMGYLSVREP
                     FRNLLTQGMVIKDGAKMSKSKGNVVDPDEIIKKYGADTARLFILFAAPPERDLDWSDQ
                     GVEGSHRFLNRVWRLVEDRLSELQSAPAEGPSLEALGDRASRDLKRVIHRTIDRVTRD
                     IEEERQFNTAVARLMELANALGDYRPADATQRALFREGVETLLTCLSPFTPHICEELW
                     AMTGHEECLARGIWPQAQPEALVEEAVTVVFQENGKVRERLEVPAGLSAEELQQRVLN
                     HPAVRDRLEGKNVIKVVAVPGKLVNVVVKA"
     gene            49101..50036
                     /locus_tag="PL162_00855"
     CDS             49101..50036
                     /locus_tag="PL162_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90608.1"
                     /translation="MPHLHVVDAGGSSQRRLISEALDRHGREGYACEGRFEHGDRPWG
                     ELFASFGGASLFEKKSVLVLEKASALGPFPPELEPSLEPPGSPTVFLAVYDGDGRKFL
                     SSEALARCTVDRVEAPPRWGAARKRWIEALAAEEGARLSAEAGALLGEWYEDPEELRQ
                     EIAKLAMASRGNEVGGALVRSLCVDEGHGLVLSLLDGVCRKNRSEVMKTLGLLRRSEE
                     LLPVGTALYNRLHIAFLATCFTGADLEKIYAAQGVRDYARRLAAEAGRHYPPRAIQRA
                     VAELIALLCAEKWGKGAGWVGMDMVLLRLLDAGRG"
     gene            complement(50239..50511)
                     /gene="rpsT"
                     /locus_tag="PL162_00860"
     CDS             complement(50239..50511)
                     /gene="rpsT"
                     /locus_tag="PL162_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HQF90609.1"
                     /translation="MPNKKSAKSRVITAERNRVYNRFWSSRCKTVGKRVMEAVAGGNA
                     EVAQNRLNEAQSVLDKAVVKGVMQRNTAARRKARLARLVKSLTLQA"
     gene            50743..59196
                     /locus_tag="PL162_00865"
     CDS             50743..59196
                     /locus_tag="PL162_00865"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HQF90610.1"
                     /translation="MTRNGKGVGRLWRGWFLLEVFLILGGAHGALGYTVGQGLLSVER
                     AEQQARSVRGVLQEYMDRTGKCVSTVDKMGYGGLANLKDEEGTLHMTPPRSGFEVPLY
                     LRIKAHLVQNPGAKISLEDMFRMGLESCGSGGGEVNLGDVLLSIHNVVRILARPENWT
                     ESDADKPGWRKIRDMKDPAWDIIQDVCGVRSAGEGKTLAEILGMRRMSDLAALSPEKE
                     EKIRLLQEKRRRDLEAAEARMAAVEKELEILRQSIPYTPYATVLPESSMEIIRKIEAL
                     SGERRALDYQIANLRENPTNNLVDRQIGFTLFDPAGGIFQPLPGAEEWVGNGGNHYYF
                     WLGSLINLVGGGKSALGAMGYERLQKYLGSNEEYARGLVQLSHFVAGSKLGSGAYGIA
                     RRCELRRLVARSRSISGMRLIPGIQGGPGGDNPDDNENIGAALLPLAPLPRKFLALCA
                     EKEPSLRPLFQGGEKEELERALDPGILGFWAERGESVEVFPGDRSAEPLAEALAFIPS
                     VAVLNDGYAEGAVALLARRGEPSVLLSPRVTPEVLARYVVLVIPSGALAEWSSSGALR
                     KKLETYVAGGGRILTLSQQLGRHYGALPGAPSAYGWAEDQSCQFGSVALTASVPAFAS
                     LTREKPHLNVDGYFLDYPPAAEVLLARTKNDQPCMISYAVGRGGVVATNLYLDWAAAN
                     HQGTEDEGLIFRDLVAWLASGAGEGESLPLGAPESPMVLKREIRYEAPSSRDTALALL
                     PTLLFLPAGSSDLREQVGASEGEKDLWRAEEGRLVLLDPDGNLLEGELFSCDLSPGET
                     RELSLKTPILKAPGFYRGFLELRGRGGADLASLPLGWVAAVGVLPALASGERRDLNFS
                     AQSDLENYVRGGVGKFSLVAWNRGNALRRLRVDYRLPHNLSYAKDPTPYQGSISLDVP
                     PGGQGSYEFSCPLVNADGIDRLWATFFDGVTGENLGTVSKGFYTRTASSVVRLSPDKE
                     SVEAGALLHVALRAGNPFDQSSSGRLAYAVTDAAGTSHALKEQDVSFTSEGYEGVVSL
                     DVPEEIFSGPGVLQAMVTIGGSMVGIGEAPLELVGPPVPLSGQVRDRFSKAALSGASL
                     TFYFGSRLFEAVTDGEGKFSLHLPGAAYKLEVRREGYVRVAREHLVYPEANPPLDILL
                     LPEGAAAGMGTVEGTCRDRVTGEPLGGVTGRFEGGGEVYDVLTDGGGRFSTTLPPGEY
                     GVTIWWGGALISDRFPLRVLEGWRQVLDVYGAVGKFSPEVRDLMTEAVLEDWRMEIFR
                     PGDEKSRRDVSAAARGGEVRTPGGGRWGFHLEKEGYAPLNTEVFISERSGAYPFFLKP
                     KAFPLEIAVRDLLTGDPLEGVEVRLDRPGGAPGPRGNTLGDGKVSLVSEEGRFTLTLG
                     RQGYGPVVTELFAGPQGGGSGEYWMRPEATPWTLEVLDIRDGLPLEGVRMRLARPDGA
                     EPQEGITDRQGQCGVSLKDGRWTVRLEREGFTTLETETWLSAATPAVRERRGAERYYL
                     APDPALPQGTLTLTVQDEYAAMPLEGVTVTARHPGGREAMGRTDAAGRVSLALLDGRE
                     AFHLEKEGYEPLDTELFFSHRGASEEPLWLTPTRASRTVLFRDLQTEALLDGVETWAL
                     EGERVDLLGRSGPEGTLAVDLPGGRRLFRFAREGYETLETEFFWAPGPVGREDIETVY
                     LTPRSRPFRGRVRDLGGTPLAGVSVTALWGETPRSTSTASDGTFIMDLPRGVGAWSFS
                     APGFKEGHAQMYWGLRSEEVPDFVLLRPEENPSGEGEVHMKVVDAVTGAPLTAFSAHM
                     GARGWMEMGVGAALVSLPAGKHSFLVQAPGYLPTGWFEESVVAGIRLGRTVALMPITG
                     ELVFHVRDALTGDPLPRFTAHLVDTGWREGEQGRITARQDGRNRDTLVKADGYFGTGW
                     FLPRAFPGRSVERTVALRPVVGELVLHVRDALTGDPLPRFTAHLVDTGWREGEQGRIT
                     ARQDGRNRDTLVKADGYFETGWFLPRAFPGRSVERTVVLWPVQGEMVFSAVDALTGDP
                     LPRFTAHLVDTGWRDVERCPVAVRQDGRNRNTLVKADGYFETGWFLPRAFPGRSVERT
                     VPLWPVQGEMVLSAVDAITGDPLPHFTAHLVDTGWREGDQGKLRVRQDGQNRNTLVKA
                     DGYFETGWFLPRTFPGRSVERTVPLWPVVGEYGVRVRDVLTGEPLATVTAHLVDRGWG
                     DAEGDALTVSQDGNNRSTLVKAPAYLESGWYLPCAVPGKRVTYPVALFPANPPGRIEV
                     RVENSATGAPISGASVSAGDGRIFRTDVSGRAFWEGTGHFVPLRFSASAPGYLGGAMP
                     SFLCTGRQGISVTLPLDEVVSPGRGGLLVVARDGEKRPLPSAKIFLGKGDQRGWLSAD
                     ETGRAVFREMGTGIYTVAVEAPGHLSWSGRAVVRPSAVTSLAPALASLSDTRRPAPFA
                     PQLVSLPGGVTLRRSETTPLVVTLRNTGEIAGRTACTLELPSVYRETRDLVLGPGEMR
                     ELDFQVMPPVDAPGATFPYTVSLEKGNALTGMATVEAPGCEVSVRTDKGAYREGDKLA
                     LTAHIVVAGGAARSEDLRETLVFRVNFNDDVWRREVVLEKGEGTVTVEDIPVAFRGNK
                     LLYGVYDLSGTARALNALYVLSDGEMNMIPDRPQYAAGEDAHIALRGTPGKAISLESA
                     LWQGMKEVSLSSEGTGEVVVPLAADMATGSYVISGDGVALRLDVRGKEIKILDRRLER
                     AGDEIRLLWSARSRGVHRCSWSVTELDREGQEGSEVASGDVELPADGSLAVVSFSEKG
                     VWGGITEGEIESPDRTSESPRKELGGYLLTLRDGALVPPLAVVRYWE"
     gene            59495..60154
                     /locus_tag="PL162_00870"
     CDS             59495..60154
                     /locus_tag="PL162_00870"
                     /inference="COORDINATES: protein motif:HMM:NF024969.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HQF90611.1"
                     /translation="MSSEKRESPDEVFRSHAARHRELVERTEEHCASALAAAAALLEE
                     TFRRGGKLLICGNGGSAADCQHLATEFMCRLRSSCQRIPFPAVALTTDTSFLTAFPND
                     YCFEDIFARQVEALCDPGDVLLAISTSGKSPNVLRGAEAARSKGGRVVALCGAGGPLA
                     EQAHVVLAVPATETMLVQEMHLMLEHALCVAVEDRFLRNPWTISGACRGKSPAEGEGS
                     R"
     gene            60151..61122
                     /gene="rfaD"
                     /locus_tag="PL162_00875"
     CDS             60151..61122
                     /gene="rfaD"
                     /locus_tag="PL162_00875"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HQF90612.1"
                     /translation="MILVTGGAGFIGSNLVAALNDRGHRDVVVVDHLARGDKFRNLRG
                     LAVADYRDREDFLRELRGGSWDRVAVRAIFHQGACADTLESDGQYMMRNNFEYSKTLL
                     HFALDRRIPFIYASSASVYGLGKRGFSEDPENEFPLNVYAYSKHLFDLYVRRLLPTAG
                     SAVVGLRYFNVFGPQEMHKGPMASMVYQAFLQLETTGTVRLFGAAEGFDAGEQRRDFV
                     AVGDVARVNLFFLDHPEVSGIFNCGTGQARSFNDVARQLIAFRGSGTITYVPFPPELT
                     GKYQNNTQADLGALRRVGYDRAFPPLEESLEAYVGILRRQEGFLTAP"
     gene            61119..62636
                     /locus_tag="PL162_00880"
     CDS             61119..62636
                     /locus_tag="PL162_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional heptose 7-phosphate kinase/heptose
                     1-phosphate adenyltransferase"
                     /protein_id="HQF90613.1"
                     /translation="MTERKGSSEEMTPFWKGEAARFLRGQGSRGSFLVVGDVMLDRYV
                     SGDVERISPEAPVPVVRVKGRRSVPGGAGNVALNLVRLGATVTLLGGVGDDGAGEELR
                     SLSEREGIRFCGVLDPSSCTVTKTRIVASHQQMLRLDEEVHPPLPEESQDRLLEAVRT
                     ALRSPFAFDGVILSDYGKGVCFPELCQEVIGAGSRAGIPVLVDPKGTDWERYAGATGI
                     TPNLRELAAVAPGVFDGRDDGGVVAAAQEVRRRYGVSWLLVTRSEAGMTLVLPEGGVH
                     ERAAAREVFDVSGAGDTVVATLVFFLAAPLPPEDAMRCANLAAGIVVGKAGTSPVTRD
                     ELLRELGSGDFPGEVREKKVAPRDEAMARVGSWRQRGLRVVATNGCFDILHVGHLSCL
                     ERARALGDRLVVAVNDDASVRRLKGPRRPVNPQEARVALLAGLSCVDLVVPFDEDTPE
                     ELYRRLRPDVLVKGGDYAPEDVVGRGYAGETVILPLVEGYSTTALLRKSAEGGGS"
     gene            62633..63178
                     /locus_tag="PL162_00885"
     CDS             62633..63178
                     /locus_tag="PL162_00885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQF90614.1"
                     /translation="MTGGRRFVLLDRDGTIMEDRHYVGDPRDVALLPRAGEGLRLLRR
                     AGWGLVVVSNQSGVGRGFFSLEAVAEVHQRLTELLAEEGVTIDAFYVCPHLPEEGCSC
                     RKPRPGMGLRAMEDFRFSPRQGLVVGDKESDVLFGKNLGMRAVLLSGDARKGSGADAV
                     LPHLESVARWAETWPEEGDEA"
     gene            63175..64125
                     /locus_tag="PL162_00890"
     CDS             63175..64125
                     /locus_tag="PL162_00890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HQF90615.1"
                     /translation="MMTEILPGERVLFVRYSALGDVIQASPLARALKAQFPGVELTWL
                     VTSPLDDLVRGQPFVDDVLVWHRSRGERGFVEMIREVRRRKFHRMVSVQSSDRSALLA
                     LLSGIPRRCGAHRWFSGVYDEGIDDLRKFLGFPPLRGALEVTEEGRVWALQELASLPP
                     RRVGFAIGASKAVKLWPDERWQELARELVARGVGVLLLGDGEEERRRGEALVRDLPFA
                     QNAVSFYSLRQTLAAVGGVDALVGGDSGVLHMAVALGVPTVGLFGPTLPAQVSLGGVD
                     CPLLADCSQRGCQRWECPEPCLKTIGTAAVLGALEELGVA"
     gene            64256..65410
                     /locus_tag="PL162_00895"
     CDS             64256..65410
                     /locus_tag="PL162_00895"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQF90616.1"
                     /translation="MDDALPFALPDMGEEETQEVVACLRSGWLTTGPRTKRFEEDMVA
                     ALGGEVHALAVCSATAGLHLALEALGVGPGDEVLVPTYTFTATAEVVRYVGAHPVFVD
                     CDESTLNMDPTEAEAKVTPRTKALLPVHVGGLPCDMGRIFDLASRRGLRVVEDAAHAY
                     TSLWHGCPVGALDSDASVLSFYATKTIATGEGGMLLTRNDDIARRARVMRLHGISRDA
                     FDRYTSPQAPWFYEVVAPGFKYNMMDLVAAIGIHQLRKAPAFRERREQIALRYDDMFR
                     DLPLTLPAHVRDGGDRHSWHLYIVRLTSDAPRSRDEFIQGLQGRGIGTSVHYIPLHLH
                     PYWRDTYALRSGDFPRAQRAYEGAVSLPIYSRMTDQDVERVGRAVRELLS"
     gene            65407..66615
                     /locus_tag="PL162_00900"
     CDS             65407..66615
                     /locus_tag="PL162_00900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQF90617.1"
                     /translation="MTGSSMPPLRRVLFICRYFPPRWNIGAVRSWGLTRYLPQFGWET
                     VLFVPPLEEGRWPTGIRIVEADWLSLGYRVKRLVGFPLPWPRRDLEDLVRREGIEAIL
                     STSTPMMAHRVGAALKKSLGIPWMADLRDLWSQNPFSRRGAFGRWRERREELRILAGA
                     DALDTVTAPGASQLAALHRRPVWHIANGFDPETVRPEGAPGDGRFTLCYTGSLYAGRQ
                     SFLPLLKALAILRQDDRLSPGDLALRIFSPPSEELRRQISSIGVEDFVIQEGMVSRDR
                     ALEAQRQASVLVLLNWEGSDAEGIMSGKIFEYLAARRPILAVGGNRTVATDLVARTET
                     GYHENDPGALAELLRRLRRSHCSGGIPFSPRKSDVEACSQVEMARKVGAVLDALTGKG
                     DASWVPRAIP"
     gene            66591..>66919
                     /locus_tag="PL162_00905"
     CDS             66591..>66919
                     /locus_tag="PL162_00905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQF90618.1"
                     /translation="MGASGDTLKIVLILPELEEGGVERHVVSLSAAYVARGHEVWVVS
                     RGGALVRALAPGVRHGTMPVHAKNPLTAAWSAIALAKEIRREKWHIIHAESRVPAWVA
                     WWASALAG"
     assembly_gap    66920..67019
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <67020..67891
                     /locus_tag="PL162_00910"
     CDS             <67020..67891
                     /locus_tag="PL162_00910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQF90619.1"
                     /translation="AESRVPAWVAWWASALAGVPWGVTAQSCYSCNAGLFPFRRADFV
                     VACSEAVRRHQEACQPGLSMEVIPDGVPSPGLSWRGPSSGDPVTFLFVGRLTAVKGID
                     LAVDAFLELPEGLPPWRFLVVGDGPLRENLENKVRRAGQEGRISFLGFQQDVFPLMAR
                     SSCLIFPSRVEGMPLVLSEAMAMGVPVLASDIEAVRGMVKESHALVSGEGAGLWAKKL
                     EEIIIKGAPPPLTPSGDFGFDSMVERYLATYRRVIEEKAGDVSLGGKAPGSEGRPGRE
                     GTNERGEGRGDLC"
     gene            67885..68682
                     /locus_tag="PL162_00915"
     CDS             67885..68682
                     /locus_tag="PL162_00915"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HQF90620.1"
                     /translation="MLKVFIVSDFTGETAEHVARAAASQFGADSTAFTRHRYVNTKER
                     VEEVVGHAWDSQAVIVCTLVDHALRKMIGELSQERGVPLVDILGPLLRAFEDRLGRTP
                     FETPGLLRRMDEEYFKRVKAIEFAIRCDDGRGAEMLPSAEVVVIGISRTSKTPLSMYL
                     AHRGFLAANVPLVPEATPPEELFQVNPAKVVGLYIMPEKLVQIRRERLRILGLDADGS
                     SYARWDRVDEELRFARDVFRRVGCRTYDVTNRAIEETAQEVLDFLRA"
     gene            68772..71408
                     /gene="ppdK"
                     /locus_tag="PL162_00920"
     CDS             68772..71408
                     /gene="ppdK"
                     /locus_tag="PL162_00920"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HQF90621.1"
                     /translation="MGEQFVYDFSQGDASMKKLLGGKGANLAQMVRLGLPVPPGFIIT
                     TEACKAYWKESEGLLDRIWPDVKAAVGRLEATTGKVFGGDDPLLVSVRSGAPVSMPGM
                     METVLNLGLNDNTVVRLGEAAKNERFAYDSYRRFIQMFSNVVLNIDSGEFENPLTQLK
                     RRLGLSYDYQIPAEEWKTLIGTFKEIVLRSTGKPFPMDPWDQLRSAIEAVFASWNIPR
                     AVTYRKIHKISDDLGTAVNVVAMVFGNLGEDCGTGVCFTRNPSHGEKHLYGEFLINAQ
                     GEDVVAGIRTPEPIADLENVMPEIYQQLLDISKNLENHYRDMQDIEFTVERGKLYMLQ
                     TRSGKRTARAAVKVAVDMLKEGLIDGKTAVSRVTPEQAEQLLHRQVDPDASVSVLAKG
                     LPASPGAAAGVVVFDPDEAERRGEAGDKVILVRPETTPDDIHGLFAAQGVLTSHGGMT
                     SHAAVVARGMGKPCVSGCEAVEIHLDQNYFRVGDVVVKEGDFITLDGSRGHVILGEVP
                     MIQPSFDDDFRRLLEEADQFSVMEVWANADTPEDSARARSFGAQGVGLCRTEHMFMAS
                     DRLPVMQRMIVAEDRDARIRELDLLQVMQTEDFRGILAAMEGFPVTVRLLDPPLHEFL
                     PKEGELRDEIAALPESDKKERAALERVLAKVLSLRESNPMLGFRGCRLGIVFPEIYEM
                     QVRAIFTAACDLTAAGKKVLPDVMLPLIGTREEMRRLKDMVDTVAREIMESHNVDVPY
                     HVGTMVEVPRAAMVADQLAEFAEFFSFGTNDLTQTAFGYSRDDAEGKFLADYVRLGIF
                     DENPFHVLDREGVGRLMRIAVEDGRRARPDIKIGICGEHGGNPPSIFFCHELGLKYVS
                     CSPFRVPVARLATAHAALASAQ"
     gene            complement(71518..>72896)
                     /locus_tag="PL162_00925"
     CDS             complement(71518..>72896)
                     /locus_tag="PL162_00925"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HQF90622.1"
                     /translation="HLLPKYGTDQVYQQGLTVHTTLDLELQHAAEEAIQGLKTEGAIV
                     ALDPKTGEVLAMVGGKNFNVSKFNRATQAFRQPGSAFKPFVYTAAAEQGIRPVDRILD
                     APLSYGNGWSPQNFDGQFHGEMTLLNAIINSYNTAAVRLAELVGPDKILDVVRRMGLT
                     TPHITGDLSLALGSASVTPLDLVTAYAPFANGGFRVSPFLVREVRSNTGEVLEQNGAS
                     LVPALAPEVAATVRSMLQDAVLAGTGSRARVKGWETFGKTGTANDFRDAWFVGGVPNL
                     VVVVYAGNDDHSPIGKGKTNTGGVIAAPVWNAFVTKAAALLGLPPTFSDKESAQVQEV
                     RVCKITGYLASKGCSSLPVLLPYDLVPTAVCPQHGGDPFEAENDLQAPRLYLAGADEP
                     LLAQYALGGASGTATAALPRASEVRPLDPIFVPPTTTQNIYEKDPSPANQIEERYQDL
                     LKQYGLSN"
     assembly_gap    72897..72996
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<72997..73856)
                     /locus_tag="PL162_00930"
     CDS             complement(<72997..73856)
                     /locus_tag="PL162_00930"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HQF90623.1"
                     /translation="MKIFVILLLVGAVALAGASVWGALYVQKLSATLPSTDEIIQRKP
                     NLATHVFDRHGKLLARLFQEDRVWVPFEKISPWMVKAILAAEDSSFYEHGGVRPFAIL
                     RSILTGDRGHGASTITQQLARNLFLSRETTMERKAKEALLSFRIEKIFTKDQILEMYL
                     NTIYFGHGAWGIGAAARTYYDKSAEQLNLGESSMLAGIVAAPEKYSPRRSPELAVSRQ
                     SYVLRRMVDVGFISPSEATEVKETPLKLTKLSKSGLESSSAPYFVSQVLFKHLLPKYG
                     TDQVYQQGLTV"
BASE COUNT        12370 a        21491 c        26342 g        14036 t
ORIGIN      
        1 aggatgatag gggaaaatgt gcggggtggg tactttttcg aggatcattt gccccttgcc
       61 tggggggatt ttagattacc aaatacaccg gtggcggtga ttcgaaaaca cgacctaggg
      121 attggaaaaa tccgtcgccg agctctggcg agggcgggtc cccttccgac gcgcgtccgc
      181 ccttttttcc gaaggcacgg cgggggcctt tggggtgaat ggggcagccc cggaggctgc
      241 tccgaagcgc ccggcgatgg cgacgatggg gggcggcggt acggcgggcc ccagagagga
      301 gcacgatggt gagcgcgatc ttccgaggaa aggcaacgct gggatttttt ctcggtctgg
      361 tggccctttt ggccctcttt cccctgacga aatcgtgggc cgtggggatg gccctttttc
      421 tcggcgccgc cgtggggaac gttcggccct ccgccgtgcc tccggagctc gggagctggc
      481 gcaagatcac tctgaacacg gcggtggtgc tcttcggctt tgggctcgat atccggcagg
      541 tcctcgccgt ggggagccag ggattctggc agaccgccgt gagccttggg gtggtgcttg
      601 ccctggggtt tgccctccgg agatttttct ccttgccccc tgccaccacg aaactcatca
      661 ccgtgggcac cgccatctgc ggcggtagcg ccatcgccgc cgtgtcctcg gtgatgaagg
      721 cccgggacga agaagtgggg gtcgccatgg gagtggtctt tctgctgaac accgtggcgc
      781 ttttggcctt tccccttctg gcgaagatga cttccctctc tccggaacag tacggcgtgt
      841 ggtgcgccct gagcatccac gacaccagct cggtggtggg agcggcggcc tttgccggcg
      901 acggggccct ttcgacggcc accatcctca agctcacccg caccctgtgg atcacccccc
      961 tggtcttcgc cctgtccctt ctggagggag gaaagggcaa gttttccgtg ccgcccttta
     1021 tcttgctttt tttgctcgcg tcaatcatcg cctcggcgtt gcccttcgcc cccctctttg
     1081 gcgccctggc gtccctgggc aaggtcttca tggcggaggc cctcttcctg gtgggtctcg
     1141 gcctgcgcgc cgcagcgctc caaagggcgg ggtggagagg ggtgctcttc gggggcgccc
     1201 tgtgggcggt gtctttgggg gtggggtttg gcctggcctc ggggagctga cggcggtgcc
     1261 ccgggcgggg gaagggggcc cacttaaggt ggccccgagg cgcggacaag gccttaaggg
     1321 aggagatagc atggacgact actacgcaaa gcaccttcat gccacgaggc ttcttttggt
     1381 ctaccagacc gacatcgagc gcgtgcgcca gtatctcgac gcggagatcg cctttgtccg
     1441 aggccatctg acgggatcgg aacgagtgct ggagctgggg gcgggctatg gccgtatctt
     1501 gcgggagctc gctccctttg cggcgtccct cgtggggatc gacatcgcgg aggattccgt
     1561 ggccctcggg gaggagtacc tgcgggacgt gccgaactgc gccctgcgga ccatggatgc
     1621 ccaccacctg gccttccagg ccgagtttga cgtggtgctc ggcctgcaaa atggcctctc
     1681 cgccatgaag ggagacccct tgcacttggt gcgagagagc ctaagggtcc tcgtgccggg
     1741 ggggcgggct tttttcagca gctacagccc ccggttctgg gagcaccggc tggcctggtt
     1801 tgccgagcag gcggagaagg ggctcctggg gcccctggat tgggagaaaa cccgggacgg
     1861 ggtcatcgtc tgccaggatg gcttccgggc caccacgttc ggggcggagg atctggagcg
     1921 cctcgctcaa ggggtggggt gcccgtatca catcgaagag gtggacgatt cgagcgtctt
     1981 cttggtgctg gaaaagtgac ccgaaggcac cccctttggc gggggagtcg ttttcttccc
     2041 ggtgcccccg gtgcccttga gaggggcgcg gtggcatggg gtgccctaaa ggcaaaaaac
     2101 cgccctccgg agggggccat ctcggctccg ttttcggagg gcggtgcgtt gcggcccagg
     2161 ggccggggga cgttatttca gactcttcag gaagcgctcc agccggttca tgccttcggt
     2221 gatctcctcc gtggcgttgg cgtaggagag ccgcagatgt cccggggcca gaaaggcgct
     2281 ccccggcacc gccgccacga gctcttcttc cagcagggcc tcgcagaagg cgctgtcgtc
     2341 atcgagcctt cgggtggcgc tctggagccc caggcagctt cgcaggtcca ccaacacgta
     2401 aaaggctccc cggggctcgg ggaagctgat gtggggcata ctccggaggc gggacatcat
     2461 gagttcccga cgggacgcga aggcctggtg catgcgcacc acgtcggcct cggccccttg
     2521 gagcgccccc agagaggccc actgggccac ggagcaggcg ttggaggtga ggtgtccctg
     2581 gaactcgtcc atcttggtga tgagggcttc ggggcccagg gcgtagccga tgcgccaccc
     2641 cgtcatggcg aaggccttgc tcaccccgtt caccagcagc acccgggggg cgaggtgggg
     2701 gaggagctcc agggggttga tgtggcgggc gtcgccgtag accaggcgtt cgtagatctc
     2761 gtcccagatg atccacaggt tatgccgttc cgccagctcc ccgatgcccc gcaggagctc
     2821 ttcgccgtag acctgtcccg tggggttgca gggggagttg agcagcaggc ccacggtgcg
     2881 gggcgtgacg gccttttcca gggcctccag ggtgggaagg taccccgagg gggccgtttc
     2941 caccaccacg ggttttccct cgaagagatt tacctgctcc aggtaactta cccaggcggg
     3001 ggcggggatg atcacctcgt ctccggggtt caccaggcat cccagggcct cgtagagcag
     3061 gggcttggcc ccggctccca ccaggatttg cttgggggcg taggtgaggc cgaaacgttc
     3121 ctgatagtac gccgccacgg cttttttgag ctcggggatg cccgaactgg cggtataatg
     3181 ggtcttgcct tgggccatgg cctcttgggc cgccgtcagg gcgctggggg gagaggcgaa
     3241 gtcgggttcc cccgccccga aggagatgac gcttttccct tgggccgcca gggccttggc
     3301 cttgcccgcc acggccaggg tggcggaggg ttccatgcga agggcgcgat gggataattt
     3361 cactgaacac cagcctcctt gggagagatt ctggaaggat tatagcccag agggggccct
     3421 cggcgccacc gctttaggcc tttctttcgg aggagaaggc cctgccttgc gatatccttt
     3481 tgccgtgatg tcagcgagaa cggagatgtt tttccgaggg aagacaggat cggggggcgg
     3541 tcagtggtgc ggctttcgcc ggtttgtggt agaatgggga gcagtggaag catgggctcc
     3601 acacgatggg aggggttttt atggatatcc gttacgtgct gaaaaacctg gaattgcccg
     3661 acgtggtgcg cgacgtcatg gaaaagaaac tcgggaaact ggagaagttc tttgatccga
     3721 ttctcgacac ccaggtggaa gtgcagcacc ggcgcggtat gttcgtggtg gagatcactt
     3781 cgaatgtgaa tggcatcatc atgaggggcg aggactacgc cccggacatc cgcaaggcct
     3841 ttgagaaatc cttgaagaac attgagcggc agatcaagcg ccacaaggat gccctcaagg
     3901 accattcgcg aacgaaggaa gcgctggcgg agatctcctt cgacatcgaa tccatccttc
     3961 ctgcggagga cgagggcgcc ggggagaagg gcaccatcgt caaggtgaag cgcttcccct
     4021 tccgtctcat gtccgccgag gaggccacca tgcagatgga ccttctgggg cacagcttct
     4081 ttgtcttccg caatgcggac agtggggaga ccaacgtggt ctaccggcgg cacgaaggag
     4141 gatatgggct cctgcagccc gataagtagc ttcgaagggc ctttcgacgt cgggggtcct
     4201 tgaatggggg ccatacgggg agggggggct gaggctcccc ctcgtgtcgt agcgtgggag
     4261 gaatgggtag gggggggagg gccatgcatg agctgtccct ggtggagtcc ctggtgaccg
     4321 ccctggaaga gagggcccgg caagaggggt gggggcggat ccagggggtg cggttgcgtc
     4381 tgggggccct gaggcaggtg gtgcccgagg tgctggagtt ttgctttcag ggggcgatcc
     4441 cgggtacgcc cctcgccggg gcggccttgg agctcgagga ggtgcccgcc ctctttcggt
     4501 gtgtggggtg cggcggggaa gtccctctgg gggggcggtg tcccgcctgc ggcggtcccg
     4561 gaggagagat gatctccggt ctggagtggg agatcctatc ggtggacgtg gaagaagagg
     4621 gttagatgcc atgaatatga caaagggccg cgagggcggc ggggcggggg atcggccatg
     4681 acccggcgcc tggagattcg ccagtcggtg tttgcccggg acgaaagggt tgccggggag
     4741 atccgccggg agatggcttc ccgggggatc ttgtttctca accttatcgg ttctccgggg
     4801 gcggggaaga ctactttgct ggagcacacc ctggggcgca cctctctccg ctgtgccgtg
     4861 gtggagggag atgtggccac ggaccgagac gcggcccgca tcgccgcccg gaagatcccc
     4921 gtggtgcaga tcaacaccca gggggggtgc cacctggagg cccatctggt gcgccggggg
     4981 ctggaggccc tgccgctgga cggggtggat ctggtggtgg tggaaaacgt gggtaacttg
     5041 gtgtgcccgg cggagttcga cttgggagaa gacgccaagg tggcggtggc gagcaccgcc
     5101 gagggggacg acaagccctt gaagtacccc tccctcttcg tgcaggcccg gggggtggtg
     5161 ctcacgaaaa cggacctcct tccccacgtg cccttttccc gggagcgctt ctggggcgac
     5221 gtggcccttc tgaaggaggg gacggaacgc tttgaggtgg ccgctccccg gggagagggc
     5281 atggaggctt ggatcgccgc gctggaacga tgggcggcgg aaaagcgtcg gcccaaggcc
     5341 cccggagggg ccccacaatg atgggggaag ggggcggcgt ggcggggccc tcgtcccgcc
     5401 gggaatcaaa gctcctggaa gggctcaatc cagtgcagcg ggaggcggtt tgtcatctcg
     5461 agggcccctt gttggtgttg gccggggcgg ggagcggtaa gacccgggtt ctggcccaca
     5521 aggtggccta cttggtggag cagggggttt cccccgggag gatcctggcg gtgactttca
     5581 ccaacaaggc cgcccgggag atgcgggatc gcatcgaagg tcttctgggg ggatccgccg
     5641 gggagatggc gatctccacg tttcattcct tcgggctgcg cttccttcag cgccacgggg
     5701 acgccctgga ggcccggggg tggcctcggc gctttaccat cttcgaccgc caggattccc
     5761 gggcgctggt aaaagatctt ttggcggagc tggagatcga tccccagagg ctcgatccct
     5821 cgggggccct ggaacgcctt tcttccgcca agggggaggc ttcggtgcgc accctggagg
     5881 cccccgccat ggatcccgca ttgaatcgcc tcttccggcg ctaccaggaa tgtttgcgag
     5941 agcagggggc cttggatttt gacgatcttc tcctcttgcc gttacacctg ctcaccgtgg
     6001 aagaagaggt gcgccagcgg gagcagaggg cttttgattg ggtgttggtg gacgaatacc
     6061 aggacgtgaa tcgcctccag taccttacgc tccgaaagct ggtggagggg aaagagcagc
     6121 tggtggcggt gggggatccg gatcagtcca tttacggctg gcgcggggcg gacatgggga
     6181 tgattctgcg cttttcccag gactttcccc ggtctcgcac ggtggtgctg gaccaaaact
     6241 atcgatcctc gggggttata ctggatgcgg ccaacgcgct gatcgaccgc aatgccgagc
     6301 gcccgaaaaa aaacctctgg accgcccgag atcgggggga accggtgcgg gtgctcctgg
     6361 tagagagcga gcgggacgag gcctctttca tcgccagcga gattgaggcc ctgcggggcc
     6421 aggggtaccg ctatggggac atggccattc tctatcgcct gaatgccctc tctcggggga
     6481 tagagcagct ccttttcgag cggcgcatcc cctatcgggt ggtgcggggc acggcgttct
     6541 acgaccgtcg ggagataaag gacgtgatat cttttcttcg gctggcgata aacccccggg
     6601 acggggcggc cctgggacgg tgtgccaacc ttcccccccg ggggatcggg gcccgggggg
     6661 tggagcgcct gggggcctgg ctcgcggccg aagggggtaa tcgggcggag gacgtttggc
     6721 gtcgcctggc ggaagagggg gcggggctga cggggaaggg gcgccagggg gcccgggagc
     6781 tgggggccgc gatggaggcc ctggggcagc ggcgcgacga cgtgccgggg gccgtggact
     6841 acgtgttgaa cgagatgggg tacgaggcct ttctccaggc cgaagagggg gactccttcg
     6901 aggaacggcg ggaaaacatc caagagctcc tttccctggt gcccgatggg gcaagcctgg
     6961 atagcgtgtt gggcgagatc gccctctata cggatgcgga gctcgcctcg gacaaggagg
     7021 acgccgtggg gctcctcacc ctgcatgccg ccaaggggtt ggagttcccc gtggtctttt
     7081 tggcggccct ggaggaatcc attttccccc acgcccggtg tgtggagatg ccggggggga
     7141 tcgaagagga gcgccgcctg tgctacgtgg gaatgacccg ggccgaggag cggctctacc
     7201 tctcgggggc ccggagccgg ttgcttttcg gcacggtgca acgccacccc ttttcccgct
     7261 ttctgcggga gttgcccccg gggcctgtgg ccctggagga tcggacgggg aggaaggaga
     7321 gtttccatgc tggttttggc tctcaccggg gatatcggcg ctggtaagtc caccctgggg
     7381 gccctctgga gggaagaggg ggcccaggta tgggacgcgg acatcttggc ccgggaggcc
     7441 tgggagctcc ccgagctccg ggcccagctc cgggagcgat ggggggacgc gatcttttcc
     7501 ccgggggggc cgttggatcg gcgagccctg ggggaacgcc tcttcgccga cgaggaaagc
     7561 tatcgctggg ggtgtgatcg ggtccatcct ccggtgataa aggccctgga agagcgggtc
     7621 cgggagtccc gccggggctg ggtggtggtg gaaatacctc tgcttttcga ggtgggagtt
     7681 ccctcctggg tggatggggt ggtgtacgtg ggggcccccg agaggcgacg ggaggaatgg
     7741 aatgcccacc gtcgtctggc gggggcggac ctggcccagc gtcagcgttg gctcctcccg
     7801 gagggggtaa aacggtcccg ggcccaggtg gtgctggaaa atgacggcga catggaaagc
     7861 ttcgtcgccg aggggcgccg cctggggcac cgctttcgcc gcatggcttc cgccgtggag
     7921 ctccggggtg tttgggccga agaggccgag gcccggcgcc tggggcgaga gctggtggag
     7981 gcccgcctcg gggcccgggt ttccgtggcg gaggagggac ggatccttcg ccggaagggc
     8041 acggtggtgg aagaacgggg ctgggccgtg acggtgacca cgctggaaga gctcgtgggg
     8101 gcggcgatgg agcgttgggg cctttcctct tctggggccc ccgatgccgt gacggtcctc
     8161 ccccggggga ggagcccctg ggaagagctg gtggagatcg aggagaactg ccggtgaagg
     8221 tggtcacgag ccacgtgggg tgcgatttcg atgccctggc ttccatggtg gcggcggcga
     8281 aacttcacga agggtcgatt ccctgtttct ccggggctgc ggggcgcaac gtgcgggagt
     8341 acttgcgccg gtttgacttc tggaagatcc gcacccccgg gaagatcaac atggacgcca
     8401 tcaccaccct ggtggccgtg gacgtgcgct cccgtaaccg catcggcccc tttcgagagg
     8461 tggcggaacg ccccggggtg gacgtggtgg tgtacgatca ccaccccccc gtgtcggatc
     8521 ccctggtggc gcgggaggag tacatcgagc ccgtgggagc cacggtgacg ctgatggtgg
     8581 agctcctcac ctcccggggc atccccatat cccccgccga agccaccctg ttctccctgg
     8641 ggatctacga ggacacgggg atgttcacct tcgccggagc cacccggcgg gactttgcgg
     8701 cggcggccct tttgcgagag cggggggcgg acggccccct cgtggggtcc ttcgtggatt
     8761 tttccctgaa ctcagaagaa cggggcctca tggaagccct ggtggcgggg gcccgggagc
     8821 gttatatcca cggcgcccgg gtggtgcttg cttcggcccg atgtgcggga tacgtggaag
     8881 gggtgtccct cttcgtgcac cgcattcgcg acgccttcga cgccgacgtg gcggtggcgg
     8941 tggtgagcat ggggcagcgg atgtatgtgg tggtgcggag ccgggagcat gtgctggatg
     9001 cggcccaatt cctggagccc ctggggggcg gagggcatgc ccaggcggcc tcggcttctt
     9061 tggcgggcga cgacgtggat cataccctac gatccctgga aaagcagctg gagcatctcc
     9121 tcgagtcggt gctccgggtg cgggacgtca tgagttcccc tgttttggcc gtttctcccg
     9181 ccaccacggt ggaggaagcg gtgcgcctca tgcttcggta cggccattcc gctctccccc
     9241 tggtgcggga cgacgccctg gtgggggtcg tcactcgaaa ggacctggac aaggccctgc
     9301 tccacggctt cggcgtggcg gcggtgggag agttcatgac ggaaaacccc tgggtggtct
     9361 ttcccgacgc cccggtggcc gaggcccatc gcctgctggt gcagggtaac atcggccgcc
     9421 tccccgtgct ggaacgggga gggctggtgg gcatcatcac ccgcaccgac gtgctccggg
     9481 ccctctaccc cacttccctc atggggtcgg agcaaccccc cgtccgggga cgggtctggg
     9541 aggaggacgt gggacccctg ctccagcgcc atctctcgaa ggaggcgtac gatctcctgg
     9601 aacgcctggg acgccggggg gcggagatgc gcctgcgagt ttacgccgtg ggaggtttcg
     9661 tccgagacct cctcctgggg gcccccgggc tggacctcga cgtggtggtg gagggggatg
     9721 cggtggcctt tttggaatcc tggcgagggg atgggtgtcg ggtggcggta cacccccgct
     9781 tcgccacggg aacggtgatc tttcccgatg ggcaaaaggt ggacgtggcc accgcccggc
     9841 gggagtttta cgaatacccc tcggcccaac cccgagtggc cagcgactcc ctcaagcacg
     9901 atctctaccg gagggactac agcgtcaacg ccatggcggt ggcgctgaac cccgaaatct
     9961 gggggacgct ggtggactac ttcggcgggc gtcgggacct gcgctatcac acccttcggg
    10021 tgctgcacaa tctcagcttc gtggaagatc ccacccgggt cttccgagga gtgcgcctgg
    10081 agcagcgcct gggctttcga ctggaagaca ccacgctgcg ccttctcaag acctgtgtcc
    10141 agagcggcat gctggggatg ctctcggggg tgcggctacg gagcgaggtg gagcttttgc
    10201 tccaggaaga gcaccccttg aaggccgtcc gtcgcctgac ctccctgggg gcctgggaaa
    10261 tggtcttttc gggcttttcc tttggcctcc gaggatcttt tgccatgcgc cgcctctccg
    10321 ccctctggcg tctgacgaaa gaagatctcc ccgacttcgg ccgggatcgg tggttgatct
    10381 ttttgggggc cctgctggag gagtgctccc cggcggggcg ccggaacgtg ccggatcgcc
    10441 tatccctcac ccccggcgaa cgaggggtgt tggggaggat catgacgggg cgcgaggagg
    10501 cggaaaggcg cctgggggga agcggagacc tgcccccgtc agccctgtgg aaggcttggc
    10561 accgagaacc cccggcggcc ctcttcttct ggggggccac ttcgggaaac tggcggtttc
    10621 gccggagggt gctcctctac ctcacccggt ggataggggt gcgtcccatg ctggtggggc
    10681 aggacctgct cgatgggggg gtccccaagg gtcccgccgt gggggctttt ttggaagggt
    10741 tgatcatggc ccggttggat ggcggtgtgg agacccggga agatgagcta gaatgggtgg
    10801 agcgcgagaa ggcccgcctc gatcggggag aagcgccccg gcaaaaaaag ggaggacaaa
    10861 aaaaactcgg gtagggggcg aagggaaaag ggcgggggcg gcacgcctcg acgaaggccc
    10921 tttgagggat gggaaggcac aagaagaaag gtgtggcgcc gcatgctagg tttgccgtct
    10981 ttgggagagc tgttgctcag tttgcccgcc gtgttgtggg ccatcacgtt tcacgagttc
    11041 tgccacggtt acgtggccta caggctggga gaccccacgg cggagcgcca ggggcgcctt
    11101 accctgaacc ccctggccca ttttgatccc ctgggggccc tcatgctcct cctgtttcgt
    11161 tttggctggg ccaaacccgt gcccatcgac acccggtact ttcgaaaccc ccgccgggac
    11221 ctgatcctcg tatccctggc cggggcgggg ggaaatatcc tcaccgccgt ggtggcgggg
    11281 aagctgttac agtggtttcc cggggtcttc ggggcaaatc aagccctctt tgacttcatg
    11341 ataatcttga tctttatgaa cctcgggttg gcggtgttca acctcttgcc catccccccc
    11401 ctggacggtt cccgggtgct ttacgtcctc cttccccccc ggatgctgga gttctatttc
    11461 cggttggagc agtatggcct ctggatcctc ctgatcctgg tgatgttcaa cgtgctgccc
    11521 gccatcacca ctccgatggt ccgggccatg gtcaccctga tcttcgcgta ggaggcgagc
    11581 gatgaagctc ttgattacca acgacgacgg cgtcttcgcc ccggggatcg ccgccctggc
    11641 ggacaccctc gtcgaccggg gaagggacct ggtggtggtg gcccccgatc gggaacggag
    11701 cagcgtgggg cacgccatca ccctctctcg ccccttgcgc ctctggcccg tgatctcggg
    11761 gctttacgag aggactcacc gggtctttgc cgccgatggc accccttcgg actgtgtcgt
    11821 gcttggcctg gaagaggtgg ccccggacgc cactctggtg atctcaggca tcaaccgggg
    11881 ccccaatctg ggggacgacc tcacctattc gggcacggta tcggccgcca tggagggggt
    11941 tatcctgggg cgccccgccc tggcggtgtc tctggggatc gcccccggag acgggaccca
    12001 ccactacgac acggcggcgg cggtgacggc ccgcctggtg gaccggctgg aaagtctgga
    12061 cctcccccgg ggagtactgc tcaacgtcaa cgttcccaac gtgccctacg gcgaactccg
    12121 gggcgtggtc cccacccgaa agggcgtccg gctctacctg gataaggtga gccgccggac
    12181 ggatccccgg gggaacacct actactggat gtccggtcgc cccgaggacg agctcacgga
    12241 gggcaccgat gtctgggccg tggcctcggg gtacgtctcc gtcacgccgg tacacatgga
    12301 cctgacccac taccccacgc tccaggaatt ggaccgcgtc gtggcgaccc accctctata
    12361 gcctccgttt ttccccggtg aggcgaggcg cgtcgggggc cgccccagaa gggctaaagg
    12421 gggcgcgagg gatcgttttc ccggagtaag gttgccgggg atccgagggg gccctgggag
    12481 ggtctttgag ggaggatttt cggtgaaaag atttttatcc atcctttttc tggggttatg
    12541 cctttggggc gggggagttt cgggggccct gggggccctc tcgggggacc gcttcatgga
    12601 cctctgtcgc agtggcactc cgaaggaggt gcggtctgcc ctaaaaaagg gggcccccgt
    12661 cgacttttgc caccgagagg ggtggacgcc cctcatggtg gccgccgcga agaatccatc
    12721 accccaggtc atctccctcc tgatcaaggc gggggcggag gtagacgccc gaaataagga
    12781 cgggtggacc gccctcatgc tcgcggcagc catgaaccct aacgccgccg tggccggggc
    12841 tttgctcgat gggggagccg ccgtggacgc ccgaaaccgg gaggggtgga cgcccctcat
    12901 ggtggccgcc gcccggaacg gcaattccca ggtggtgtta gcccttctca agaaaaaagc
    12961 cgacgtcaac gctcggaaca aagatggatg gacgcccctc atggctgcgg cggcgaagac
    13021 ctcccggggt gagatcgtcg ccgccctcct caaggcgggg gcggaggtgg acgcccggaa
    13081 caaggacggg tggaccgccc tcatgctcgc ggcggccatg aatgcccacc ccgagatctc
    13141 ggagaccttg atcgaagcgg gggcagaggt ggggcttcgg gacccccggg gcatcactcc
    13201 tctcatggcg gcggcgacga gtaacgccaa tcctcgggtg atagcccgcc tcctagaggc
    13261 gggggcctgg cggggcgcct cggaggaggg ggaatggacc cccctcatgg ccgctgcctg
    13321 gcacaaccct aaccccgatg tgatcgccgc cctgctccga ggaggccttt gcgtggatgc
    13381 ccgagatcag gagggggcca cgcccctcat gatggcggcc cgagagtcct ctcccggggt
    13441 cgtggaagcc ctgctccgag gcagccccga cgtgaacgcc cgagaccagg aggggcgtac
    13501 cccccttgtt tttgccgccc agggggcgtc gcaagacagg accatccgcc tcctcgtcgg
    13561 cgcgggagga gacgtgcatt cccggagcgc cttgggatgg actcccctca tgtacgctgc
    13621 ggccatgaac cccaaccctt cggtgacggt gtccctcatc gaagccggag ccgaccccaa
    13681 cgccacaagc ggcgagggct ttcctcccct tctttttgcc gtcctggaga acccgaatcc
    13741 ccaggtggtg gccgccctaa tccgaggggg agccgatccc cgggcccgct ttccccacgg
    13801 ccgtaccctg cttatgatgg cggccctcaa gggatcccat cccgaagccc tcctggagct
    13861 cctccggggc ggggccgatc cccaggcccg ggatcaggag gggcgtcggg cgctggaata
    13921 cgcccgggaa aacccccgtc tccgagacac ccccgcctac tggaagctca acgacttgag
    13981 ctactgaccc ctccgtcgcc gatggataga cgcgtagggg gcggtaaaag gggttgccga
    14041 aaattttttc ctaccccgtt gacaggggag aaaaatctga taccatacga ttcatcttag
    14101 aaagagcggg aaggggggct ggcgatgagc ggcacggtgc ggcgacgacg acgaggacga
    14161 tgcgactttt cttctttgcc cataacggcg atgagggaaa agagtatgct gtgcgctcgt
    14221 cgacagagag aaaacgccct gggctgaaag cgttttccgt aggccgcagc agaaacgcgt
    14281 tcctgagctt cttccgaaga aaggggtctt tccccaagta ggaggacggg gggttcccgt
    14341 tacagggcct caagagaggg cggcgaagtt ttagccgcca aaaagggtgg taccgcgggc
    14401 ttatggctcg tccctgaggg ggacgggcca tttttttatg gagatagaaa aaggaggaat
    14461 aaaaatgtac aaagggaaag tggtattggc gttcagcggc ggtctggata cttccgtggc
    14521 gatcaagtgg cttcaggatc agggatatga cgtggtgacc tatacggcgg acgtggggca
    14581 gcgggtggtg gatctcgacg gcattcagga gaaggccctg aagaccgggg cctgcacggc
    14641 ccatgtccag aacctccggc aggagttcgt ggaggacttc gtttggcccg ccctcaaggc
    14701 tcaggccctt taccagggga cttatcctct gacttccgcc ctttctcgcc ctctcatcgc
    14761 caagcatctg gtggaagtgg cccaggccga aggcgccgtg gccatcgccc acggctgcac
    14821 ggggaagggg caagaccagg tgaggtttga agtttgcgcc ggagccctgg acccttcact
    14881 taaagtcatc gcccccgtgc gggactggca tttttcccgg gaggccgagg tggcctatgc
    14941 cgaggagcac ggcatccccg tgctccagca cgtgaaaggc atctatagca tcgatgaaaa
    15001 tctctggggg cgcgccatcg agtgcggtcc cctggaggat ccctggaacg agcccccctc
    15061 cgatgccttc cagcttacgg tggatccttg gaacgcgccg gatgaacctc tctacgtgga
    15121 gatcggtttt gaaaaagggg tgccggtgtc gctgaacggt aagcccatgg acggggtgac
    15181 cctgatccag acgctgaaca ccctggcggg ggggtatggg gtggggcgga ttgacatgat
    15241 cgaagaccgg ctggtgggct tcaagagccg ggaggtgtac gagtgccccg ccgccgcagt
    15301 gctcatgcag gcccatcggg tgatggaaag ccttaccctc agcaaagacg tgctccaggg
    15361 caaggaatcg ctctcccagg cctacgccaa gatgacctac gaaggctact ggtacgcccc
    15421 ctacaaagaa gctctggatg ccttctttga ccacattcag caatacgtca acgggaccgt
    15481 gcggatgcgg tttttcaagg ggcaggccgt ggcggtgggg gtcaagtccc ccaatgccct
    15541 ctatcgggag gatctggcca cctattccga ggcggatacc ttcgaccatt ccgctgcggt
    15601 gggcttcatc tccatctggg ggatgcctat aaaaacttgg cgggaggccc acccccacga
    15661 ggcgcccctg gagatgatcc ccctccgggc ggtgggagag gagtaggccg gtattcccgg
    15721 tattcccggt attccatggg tgaattgcgg gggggggcag ggatagcccc ccccgcaacg
    15781 tctttggctc ccgaaagatc attggggggc gtcggggtcg tccctccagg cctttttgat
    15841 ctcttgaagg cgaatggtgc ccttgaacgt gcgaaaaacc tggccgaagc ggagaaagtc
    15901 ggggcgggcc cccttggaaa cctcttctcg ggcctccacc aagaaggtgc gccggagctc
    15961 ttcttcgagg ctccgggccc ggtcactttt gagttccagg gtgacgccac aggagtcctc
    16021 gtgctcgctc gcgatgcgga gccccgccac cgccaggtcg aagtcgtcgc aggacagggc
    16081 gtagcggcgt tccacgaagc ggcatagttc gccgttgatc tggtcgcaag cgtagtttgc
    16141 ccctccccgg atgagaaggg cggagtctcg ccccacccag tagatatccc tctggccgtc
    16201 ctgagggtgg gtgagcccga aggccacatc gaggagcccc agataccacc cctcccgaag
    16261 cacccgccgg gagccctctt cgtcccgtac gtatccggtc atcacgtttc ccccacggca
    16321 gatcatgtat cccgggcgcc cctcggtgca gtcccgcaga tagccttctt tatccggatc
    16381 cacctcttcc acgatgcgca cctccgtcag cggcgggtga gggcgcccga tccagtatcc
    16441 cgggggagaa tggcgccatc cccgctcgaa ggcccgtcgc cgggcgtcgt ttgccatggc
    16501 taggggggtg ccgaggacgt gaaaacaagt ttccgtagag ccgaagcgca cgctagggac
    16561 ccgtcccgtc catcgctcga ggcgttccac cgtggtgggg cccacggggg cggatcccag
    16621 gacaaactcc acccgcgcca tgctctggag taggcgcggt tcctccacgg ggagggaggc
    16681 cgtttcccgc agatgttcca gatagtcgaa atggcgcgcc accgtggggg cgatgatccg
    16741 ccgggatctt ccttctccct cggcgcactc cgtcaccgcc ctccagtaaa atgtgccgta
    16801 gcgcggaaag agcacgagct ccgccccggg ccttcggagc accaggtccg acaccgccgt
    16861 ggagttggcg tgatgcaggg ggttgatcat gagaaagagg gggccttgct ccgggggatc
    16921 ctccggaagc tccaggtagc gtcccagggc ttggtcgtta ttatcgtaac tgccgtagga
    16981 caggcgtgcc cccttggggt gtcccgtggt gcccgaggtg aagatgacta tacggtcgtc
    17041 ggcgcttgtc aggtcggcga cgaaggcccc ttcttccaag ggactttctt cggccaagga
    17101 ggagacggcg tagtccggaa ggcgggggaa gcggcgtgaa agctctttta gaaaatcggc
    17161 gccccccggg gctttgaggg cgaggaacga agggtcccaa accatgaggc gtccctccgt
    17221 gaggtcgatc ttgtaggcca tttcttccac tccatcggcc tgccagttca tcgtcaccgg
    17281 caccgccccc accatggtgg cccccaggcg aaacatcaga tcctcggggg tgttttctcc
    17341 gtaggcgttg acgaagcgat ctcctttgcc gagaccaaag cggcgcaaca cccccgccgc
    17401 ccgcagagcc atgagccaaa actcccctcg agtccagcgc cttaccgact cccggggggc
    17461 gggggagacc gttcgcagga agggctcctc cgggcggccg aaggcgggaa ggtacctacg
    17521 cagtgcttcg gaatacatgg gcttcacgtc ccttcctggg ggcggcgcgg cgttccacga
    17581 tgatgacgat ttcatcgctc cggggtccgg aagtaccggc ctttcggcgg ggtcggagtc
    17641 gtggggcttt tttcgcgaaa gtccggatgc tagagggatc ttttttccat gtcccccagg
    17701 gagcggcgga tgaaaaggga agggctcgtg gaggtgtcca ccaccgtggc gggaggggcg
    17761 gagaacccgc ggccgtcgac ctgcaaaagc accagggggc gcatgagatc tccttccttg
    17821 gcggccacca cggtacccac gctgccgtcg gccagttcca ccagcatccc cggaggatag
    17881 agtcccaggg cgaggagcat ggttcggacg acagcggggt cgaagtggga tcccccgctg
    17941 gtgactatca tggaaatcgc ctggtcccgg gctatgggac ccttgtacac ccgggcggtg
    18001 gtcagggcgt cgaagacgtc cgccacggcg gagatgcggg cgggaagggg gatttcttcg
    18061 ccatttagct tatggggata gccatccccg cgccagcgtt cgtggtggaa gcgcaccacg
    18121 gccaaggcca cctcgtcgac gacgccgttc tggaccgcca tggagtgccc ttcctccggg
    18181 tgtcgttgga tgacggcgaa ttcttggtcg ttcaacttcc cgggcttgtt gaggacctcc
    18241 cggggaatga gggcctttcc caggtcgtgg agcagggcgc cgacggtgat ggactccacc
    18301 gccgccgggt cccccaggat gcggtgggcc agaaagccgc tcaggagggc cacgttgagg
    18361 gaatggacaa aggtatactc gtcctgctcc ttcactcgcc ctagggagag ggagatctgg
    18421 gtggtgcggg cgatctcccg ggccaaggtc ttcccggtgt ccataagatg ccccaagtcc
    18481 tccggggtga cttggtggtt ctcgatgcgg gaatacacgt ccccgatctg ccgtgtggca
    18541 tggcgggcga gggagtggtt cacctgggag agctgggggg gggagagggt ctggagcact
    18601 tcctccaagc tttccatgga agtttcaagg gagtcctgta cggctacgcg ccctatgccc
    18661 gcttccgaca ggcgtcggac cacggagggg agggaggtct ggatccatcg taactccgtt
    18721 cccttgggca acagcaacgc cttggtgggg gagacgatat cttccgccac cgtgcctcca
    18781 caatcggcaa gctgagctat ggggatgaag cggactcgtt ccaaaggccc ttcctcctag
    18841 ggatagtttt aacatggaac gggaacgaag gtattataat gcctaaaggg aaaattgtcg
    18901 cggagagatt tgtcatgttt tccaaaaagg aggagaatgg catgatcctg gcggtgagtg
    18961 gactcaaaaa cacgggaaaa agcactctgt gtcgcttttt agtgcgctat ctgggggatc
    19021 gaggactccg ggtggggtac gtcaaacaca gcgatcacgc cctttttcgg gaggagggca
    19081 ccgacacggg aaagatcctc tcccaggagg gcatcgcggt ccactggggc gccgacggct
    19141 ttcgcctgga gcgtcggggg gatctctctc cccaggatct ggtccgggag tgcttccccg
    19201 ggggggatct ggtgcttctg gaggggggta aggccctacc cctgccccgg atctgggtgg
    19261 gcgatccgga cgacctcccc gaggggacct cgggggtggt ggcgtggtac ccgggatcga
    19321 gtaaaaaaat ggagaccccg ggggataata agggtgtgtc ctgcttcgat cggccggagg
    19381 ccctggggga ctttgtggaa gggctcgttc gaaaaagcgc ccgaagcgcc cgggtcttcg
    19441 tgggggagaa gcccttgccc ctcaaggact tcgtggcgga cttcctgcgg ggaagcatcg
    19501 tgggcatgtt gcgatccctg aaaggggtgg gcgatccgga aaaagagggg atcctcgtgg
    19561 cctttccccc ggagcgcccc cttggggata aaaggcccct ctgacgcctt aggggcgccc
    19621 tctgatcata agtttttttt cggaaaagtg cgacggttcg cggggcctcc cccgccgccg
    19681 aggtcttaaa aaagttcccc tcccgtaagg gatcgtcggg gtggggggga tccggcgggc
    19741 cctttgcccc tcgttagccc ctcttaggag gaataaatgc ttgccaccac cgctataatc
    19801 ctgatgtttg tggtattttt ggccctcatt tcccgtaaat tgaacgtgcc tttgattctg
    19861 atcgctctag ccatcgggat tttctttgga agcgacgtca ccggcctcat atacttcgat
    19921 gacgcctccc tgacccagag ggtggccaac ggggcgttga tcttcatcct tttcgccgga
    19981 ggttttggta caaaagaaga tcacctgagg cccgttttcg agatcactat gctgctagcc
    20041 acggggggag tgcttcttac cgccgccgcc gctcttttgc cctttgtttt gataacggga
    20101 ttcgaggtga aaacggccct gctgctgtgc gtcatcatct cctccacgga cgccgccgcc
    20161 atcttttcca tcctgcgaac tcggtccatc aacagacgtc tcacttccat caccgaaata
    20221 gagtccgccg ccaacgaccc catggcaatc atttccacgg tatttgtcat ccagctcatc
    20281 accggggccc acgtgggaac cttggagacc gtgacgagct ttttgtggca gctcctaggg
    20341 ggagtggggc tgggggtcct tttcggcctg ggaggggtct tcgtcttcaa gaagatccgg
    20401 gacatcgacc tggggtattt ttacctcttc ctgatcgcct tgatcatctt gacccacgaa
    20461 gtggcaaact tctgccaggc cagcggcatg ctcgccgtgt tctttgcggg gctcaccatg
    20521 ggaaacaaaa gctttcccta caagtatggc atatcctcct tcaccgaggc tctttcgttt
    20581 gtggccaacg tcctactctt cgttctgttg ggacttttgg tctttcccaa ggattttccg
    20641 gccatttgga tgcccgccgt ggggcttttc gtcgccatca cctttgtggg aaggcccgtg
    20701 gcgatcctcg tctgcacggg gttggctaag ctttccctca aggacaaggc gtttctgtcc
    20761 tggagcggca tcaagggggc ggtgcccatc gtgctggcca cctacccggc ggcggcgggg
    20821 ttggacccca atcaccatat gtttaacatc gtcttcctgg cggtgctatg ctccatcctg
    20881 acgcagggaa ccaccatagg ccggttggcg gacgccctgg ggttggccgt gaagggacag
    20941 aaaaaatccc gtcagagcat ggagctgatc accgcccacg agaccagcta cgagctcatc
    21001 gaggtttaca tcgacgacga cctctaccag ggagagtgtc gcatcatgga cctatccctc
    21061 cccgtgggga ccaccatcac catgataaat cgggacgacg tcatcatcgc ccctgcggga
    21121 aagtccaggc ttttgcccgg ggatattctg tccctcctcg tggacatcgg caacgtggac
    21181 tccgccgtgg gggagatcct cagcaagttc ggaaagaagt agatcccttc tggcgtaccg
    21241 gggggctttt gggtcttgac gcccccccgg cgggcgtgat aagattcccg ccgttgcacg
    21301 tggccacacg agtaccggga gtcgttagct caggcggtag agcaccggac ttttaatccg
    21361 ggtgtcctgg gttcgaatcc cagacgactc accagcgcgg tcccgtcgtc tagtggtcta
    21421 ggacacagcc ctttcaaggc tgcggcacgg gttcgaaccc cgtcgggacc gccaatttgc
    21481 cggtgtagct cagttggtag agcagcgcac tcgtaatgcg caggtctccg gttcgagtcc
    21541 ggacaccggc tccagcgccg aaaacgcccc cgcgcccaag atcacacagg gccctgcggg
    21601 ggatcatttt tcccctcccc ctctctctcc ctccctcttg tcactatatg cggcgcccca
    21661 aaatacatcc caaggacaaa cgtattcggc ggcaattccg gctacaggta tgatacttcc
    21721 ggagggtggc cgttttgggg ttttcgaccg ggagatgatt ttccagggga tcatgacttt
    21781 tggcgtcgac tcctcgacgg gggaagcttc tggtataatc actctcggct tgctccggag
    21841 aaaggagtga gccggaagct ctcgaaggtc gagatcgggc cggattcggc aaggaagcgg
    21901 gatggcggcg acgcccgaaa aggacggagc gccctctgag gggtggtgcg accttggttt
    21961 cagaagaagc acagggggcg atgttttcgc cccaggattc ggaaggagag ggggatcgcc
    22021 ttctctggcg gcggtgtaag acaggagacg aaggggcgcg ggagtccctc atcctcaaat
    22081 accgtccgtt ggtcttttgg tttgtccgcc agttccaggt ttctcggtcg tcctttcccg
    22141 atcttgtgca agaggggatg atggccctca ttgaggcggt ggaccgtttt gacccggaaa
    22201 agggcacccg gtttagcacc tacgcctatt atcgcgtgcg ggggcacatg gtgaatttcc
    22261 tccagcgtag ggagtctcgg gcccccttgc cggtggagga cgtggagccc ttcatggagc
    22321 agaacgatga ctccatgtct ccggactggg ccttggccct aagggacggc atggcctcct
    22381 tgagcgaccg ggagagcgcc gtgatcgacg agctggtggt gcaggggacg agggcaaaag
    22441 attacgcgca agagcttcgg gtggatgtga gctacgtcta ccgggttcag cgacgggccc
    22501 tggcgaagct gcgcctctgg atggggttgg aggggtccac aagataaggc gctccgggga
    22561 taaccttgtc atggtgggag gagagccttg ctccgatccc cccaagagga cgagggcgtc
    22621 ggaataaggc ggcggttccc ttggaagtga agggtgtggt gccccgtgga gcgaaagatt
    22681 aggcgtgtgc agcgatggct tgaccgttgc ctggaagcct gtagaattgg tcagtggcag
    22741 gcagcgttgg ccgagatgga gtgtgcccgg acggacctag acctcgcccg tcgggccctc
    22801 tgggatgagc tttcggccgt ggataaatcg acccggaggc gtccctttcg aggaggggcg
    22861 gccctgcgag gagccctggt gggggccttg gtcatcctgg cggtggctca ccccctggcc
    22921 ttcgatgacc cctccggggc cccgtccccc tctcttgtgc cggagatcgt ggccttcgtc
    22981 acccgggatg agcagtcttt gattctggcc ctgaggacga ggttgggcga aggggccctt
    23041 cgccaagggg cgggggaatg gtctcagctc tcttccgaag aggagggcgc ccttcggcgg
    23101 gacgcagggg cccgagcggc ggggcgcggg gtgccgccgg agaggaagag cccctctggc
    23161 gcggggcgga gcgcggtttt aaaagaggac ctaccccggg caacagaggg gggaggcctt
    23221 ccggcggaag atcttctggc gttggtgcag gtgggggagc gagcactccg ggatgcgtcg
    23281 gtgcgcgtgg tgggggattg agacgaaacc ccgcctctcg ccctttttct agcgaaggga
    23341 gttgggcgta gggtgaagcg gttgatggcg tttcttctgt ggggagctct cctgggatgg
    23401 ggaggcgcgg cctgggcggc ggagccgctg gtggtctctc tttccgtgga gggcaatagc
    23461 caggtggtgg cggcccacat cctgagcgcg gtggatacca aggtggggga acctctggac
    23521 cgggagcgac tgaaaaatga cgtggaggcc atctacgagc tgggcttttt ctccgtggtg
    23581 gacagccgtc tgtcggaaca gtccgacgga gtggccgtga cctttcttgt gcatgaaaat
    23641 ccagtaatag aagacattcg cttcgagggt aacacggtgt actcggcgga ggcgttgcgt
    23701 tcggtgctct tcaccgcccc ggggaacgtc ttcaaccggg tgtttttcca gcacgacctg
    23761 gatcgtatca aggagaagta ccaaaaagac ggctacgtca tgatgcgggt cctcgacgtc
    23821 caggtggacg ggggcgtcat cacggtgaag ctcgtggagc cccgggtcgg agatatcatc
    23881 atccagggga acaccaagac caagacccat gtcatccgcc gggaactccg ggtcaagaag
    23941 ggagatcttt tcaacgccac cctgctgcga ttttccatca accgcctgca ggggctgaac
    24001 tatttcgaag acgtgagcgt gggcttcgag cccggagagt ccgacggaga ggtaaacctg
    24061 attctcacgg tacaggagca gaagacgggg cggatgacct tttccatcgg ccacggctcc
    24121 gccagcggat ggagcggcgg cctcggctac gatgacagca actggaaggg gctggggcac
    24181 aaggtctccg tggggttcga gacgggaaag aaagagcagt attggatcaa ctacaccgaa
    24241 ccttacatgg acgataccca ctacgcatgg aaggcgggct tttacaagcg tacctgggag
    24301 gacctcatgc gctatgccag ctctgaccag ggggacgacg tggagtcggt gaagtacgac
    24361 cagaaaaaga cgggggtcta ctacggcttc gggaagaagt tccgggggaa ctccaagatg
    24421 agctggttcg tcacgctgga ctggcacgat acggaggtca tccccaagtc gggggatttt
    24481 gatccctact acagccgaaa caatctgttg agcggcaaga tcttcacggt gcaggccagc
    24541 cttaccagga acaacctgga cgagtatacc ccttatccta agggagatct ggaggtcctg
    24601 acggtggatc aggccctgga gacgctggga ggggagtaca gcttcacgaa gtacagcctg
    24661 gaagcccgat ggtacctccc catccgggga ttggcgcgct acgtggatct cttcgacaag
    24721 gtggatgagg aagaaaatcc catcatcttc gccactcgca tgcgggcagg attctcttcg
    24781 ggggccctcc cctgggcgga acaatacttc gtgggcggcg gcaccacggt gaggggattc
    24841 aaggatgacc gctacgaagg ggacgagatg tttttggcca acgtggagct tcgggtgccc
    24901 ttcgagaaga acttttcctt cgtggttttc tacgacacgg gcatggcctg ggacaaggac
    24961 ctgggagaga gcttcagctt cagcgagctg aaaggggcct acgggatagg ggtgcgggtg
    25021 aaaacccccc tggggaacat ccgggtcgac gtggccaacg gtgacaacga gacggaaacg
    25081 caattcggct tcggcgagct cttctagggc ctttgccggg cttttggaga tcggaagaag
    25141 ggctgcggcc gcgctgttgg tcggagccct ttttttcgtg gtgccccaag aaagcttcgg
    25201 ggcggtgcgg gtgatcggag tgcccctttg gctgcggagc acggcggaac ggagcctgga
    25261 ggcggtgtgg caggagctcc ggtcccgccc cgggagtccg caggagcatt tggagatctt
    25321 gggcctggtg gcggggcgtc tctttgcggg atacggggta aaatcggcct ccggagaggg
    25381 ggaccccgtg gtggttctga tccccgccgt ggctcctccg aggtggtcgt tgcgcctgga
    25441 gggccccgcc ctttcggggc ctccagggga atggctttcc tccgacgtgc gccggacaga
    25501 aaaagccctg gctcccctct tggacgaagt ccccgtggag gccctgggat ggggagacgg
    25561 ggcgttgcgc aaggtgctcc aagagacggg agaacaggag ctaccggggt gggagttggc
    25621 gcccttggtg cagctttcgg gaggacaggc ggagctccgg gtgcgccttt tgccatcgcc
    25681 cccgttggtt ttagccctgg ccccctcgct gcgatccaca tcgcttcctc taatgcttaa
    25741 aaaagatctc accgaggctc tgctgaaaaa cctggcgccg atccatggcc tacccgtggc
    25801 gtgggtgcga catcaccagg aagaggccga gcggtgggcg ggagacatcc tggaggaccg
    25861 taacacggtg caaaacaccc gggcctcggt ggcggtgcgg atgaagccca aagagatcgc
    25921 cccggtggac gtacgggtgg agagtcggac ctacgtgctc caggcctgga tggcggccta
    25981 tgcggggagc gatgccctct atcccgaggt ggggatccat ctggggcgtc gggcgtccct
    26041 ttggtcccgg ggagaggtgg agctctacgg tgagtggatc gccaaggtgc acgacttcgg
    26101 cctggaaagc cgatgggggg tgcgaacgtc ccccgtggga gacctttggc tgggggtgga
    26161 agtggtctgg ccgggggaga ccgtctgggg gcgcctgtgg ctggaaaacg atgcccggcg
    26221 ggcctatgcc tggtggcgtc tgagccgcga tggcgataac caagggagcg tggggtggca
    26281 cctgacggac cacatatcgt tggagcttca ctatgatgat cgggtgcccg atcaattgat
    26341 cctgcgggcg gtgggcactc tgtaacccca ccgagggggc atctagggca acgaaagaag
    26401 gcatctcttc agtcgaggtg gtggcgcaga tgaaggcgaa aaagcccttt tctctgggcg
    26461 atgcggcccg gatgacccag ggcgccgtcc ggggggacgt ggaccggcaa ttccgagggg
    26521 tctgttctcc ggggcgcctg cgagaggact ttctagtggc ggtatgggac cgccgctgtt
    26581 tggaggacgt acccccttcc tggggcgtga tgacctttcc ttcttgggct ccggaagggc
    26641 aggatgccgt ggaggtggag gatccgcgcc acgccctggt gctactgctc caaggggtgt
    26701 acgaagcccc gccccgcgcc ggggggatcc acccctcggc ggtggtctcc ccggaggccc
    26761 aagtgtcccc ccaggcctgg gtggggcccg ggtgcgtggt ggaggagggg gcggtggtgg
    26821 aagagggggc gatgctggag gggcagatct tcgtggggcg cgacgtacac ataggatccc
    26881 aggtgtggat cgccccccag gtggtgctct acccccggac gacggtggga gcccggacgc
    26941 gcattcactc gggaaccgtc ctgggagccg atggctttgg tgtccttttg ggagacgagg
    27001 gggcccggga ggccattccc cacgtggggg gcgtgctcat agaggaagat gtggtgttgg
    27061 gggcccacgt caccgtggat cgaggcaccc tggacgtcac ccgggtgggg gcgggaacca
    27121 aggtggatga ccacgttcac gtgggacaca atgcggatat cggccgccgg tgtgtgttgg
    27181 tggcctacgt gggcatcgcg gggagcgcgg tggtggagga cgggagcatc ttggcggccc
    27241 ggagcggcgt ggctccccac gtgcggatcg gtcagggggc ccagctggcg gccatggcgg
    27301 gggccacccg ggacgtgcct cccgggggag tggtctctgg ttttcccgcc tgggatcaca
    27361 aaaaggccct gcgggtggag tcgctgatgc gccgtttgcc ggagctcttc gagcggctga
    27421 agcgcctgga gaaggggcgt tccggggagt cgtcatgagc cgcacggtgg cccggccggt
    27481 gacgtttttc gggcggggac tccatacggg ggagagctgt cgggtgacgg tccatccctc
    27541 atcggaaggg ggggtgcggt tccgcctttc ggggaactcc ttccccgtgg aggaggcccg
    27601 cttttccgga gacggccggg gcaccacctt ggcctttccc cgagggggct cggtggccac
    27661 agtggagcac ctgatgggaa ctctctcggg gctggggata tggcacgcca cggtggaggt
    27721 ggagggcccc gaaatcccgg tggaagacgg cagtgcggcc cccttggcgg cgctgatcct
    27781 ggaggcgggg acagggcctg cggccccggt gattccttgg gccgtgcctt ttcccgtggc
    27841 cgtggacgac cccgaccggg ggcgttttgc ggtggccctt ccgggggaag ggtgtaagat
    27901 ttcttacgtt ttggactacg cctccccttt tttgggtacc cagatccagg atctagagtg
    27961 gactccggag tccttttgcc gccaaatcgc cccggcccgg acttttgccc ttctggaaga
    28021 ggtggaagag cttaggcgcc ggggtctagt ccggggaggt tctctggaga actcggtggt
    28081 ggtgggggcc gagggcgtga tgaacgccgg agggtggcgt tttccccggg agggggtgtg
    28141 ccacaaggtc ctggacctca tgggggacct ggccctggtg ggacgccccc tggagggaag
    28201 atttttggtg tgccgggggg ggcatgcgct gcacctggcg cttctgcggc gtctccgctc
    28261 cctgggggcg gggggcccga agcggtgagg ctccatccct tcgctccggg cctttggcta
    28321 cggaggatgg gcgccgccct agagggccta cggaccaagg gcccccggcg cgatggtgcc
    28381 cccccggagt tcgcgcgggg ccttagagat cgaccgtcga aggagggttt taggccgtga
    28441 taaacattca agagatcttt cgcctgttgc cccaccgtta cccatttttg ttggtggacc
    28501 gcatcctgga ggtggacgga gagcacgtca tagggttaaa aaatatcacc atcaacgagc
    28561 ctttttttgt ggggcacttc cccgacgaac ccgtgatgcc gggggtgctc ttgctggagg
    28621 ccatgggaca ggtggcggcc atgctggtgg tcaaccttcc cgaggcctcg ggcatggtat
    28681 cttacctcac gggggtgaat aacgcccgct tccgacgccc ggtcaagccc ggagatcagc
    28741 tgatcaccga ggcccggctg atcaagttcc ggagccgggt gggcaaggtg caggtgacgg
    28801 ggcgagtgga cggagaggtg gcggcggagg cggagctgag ctttgtcttg gcccagggcc
    28861 tttcccggga ggattccaag gagtcgtagg cctttgggct ccggccagtg atccttcggg
    28921 gcccgggagg agcggtgccc cgggggcggg gttcgatgtt tcgtgcgggg agaagggacg
    28981 atggtcatgg caccgcaaat acatgccacg gcgctggtgg caccgggggc ggagattgcc
    29041 gccgacgtcc gcattggccc ctactgtgtg gtggatggtc gggtgcggat cggcaagggg
    29101 agcgtgttgc acgcctttgt ccgtctttgc gacttcgtgg aggtggggga ggggtgcgag
    29161 ctctgcgagc atgtcaccct gggaagagaa ccccaggact tcggtttccg ccacgaggag
    29221 agctgggtgc gcatcggctc tggggtggtg cttcgggaga acgtcacggt gcatcgggcc
    29281 acgggggagg ggaacgccac ccgcgtggga gacggagcct atctcatgga aggggtccac
    29341 gtggggcata acgtccaagt gggccccggg gtggtcatgg cgagcaaggc gggcatcgcg
    29401 ggctacgtgg agatcgggga gaaaacggtg gtggggggca tggtgggcat tcaccaattt
    29461 gtcaaggtgg gacgttactg catgttgggt ggggcgtcca agatcgtccg ggacgtgccc
    29521 cccttcgcct tggtggatgg tcgcccggcc cgaatgtttg gcatcaacaa ggtggggctc
    29581 cggcgccatg gattcggcgg agaggcccgg cgccacgtgc tgtccctcta tcaatccctc
    29641 taccaatcgg gtcttcccct tcgccaggcc ctggaagaga taaaagcgaa cttggccccg
    29701 gaggacgccc taggtcggga gatcgtggcg tttgccgagg gagcccatcg gggcttggtg
    29761 ccctggggac ggcggcacga cggaggggat catgattaaa ctttttgcca tggacgtgga
    29821 tggtaccctc acggacggag ggataatcct ccacggatcg ggaggagaga gcaagcgctt
    29881 cgacgtccag gacggcctgg gtatcgttcg cttacaggcc gccggggtgg aggtggcctt
    29941 tgtgagcggg cgccgaagcc ccgccacgct tcgccgggcc gaggatctgg gggtgcgggt
    30001 gtgtcgccag ggagtgaaag acaagctcgc cgcccttcag gatctggcaa aagaccgagg
    30061 tcttagacct tcggaagtgg ccttcgtcgg ggatgatctg aacgacgtgg catgccttcg
    30121 atgggccgga tggggggtgg cggtggccaa cgcgatcccc gaagcaaagg aggccgccga
    30181 catggtgacc cgagcttcgg ggggggcggg ggcggtgcgg gaggcggcgg agttcttgct
    30241 caaacgcctc atggcggggg actgatccat ggggatcttc tcgggaaaaa ttctcgatcg
    30301 ctacgtactg ggagagatgg gtcaaccctt ttgctttggc atgatggcct ttgcggtgct
    30361 cttcgtggcg ggggggttgc tctttgagat cgccgacctc atcatcgagc gaggcgtgtc
    30421 gttttacgtg gtcctgcgcc tttttctata ccgcctgccg tcggtggtgg tggtggtggt
    30481 gcccatggcg gcgcttttgg cgaccctgct gagcttcggt cgcctttcct ctcagagcga
    30541 actagtggcc ctcaaagcgg cgggggtctc tttccaacga gtgctgcgcc cggtgctgtg
    30601 gggggccttg ggggtgactt ttgccaccat cctctttaac gaaaccgtgg tgcccctttc
    30661 caatgcggcc acggatcagc tcatgcgggt agaggtctac cgccaacgcc cttcgctcat
    30721 caaggagcgg gtctttctcc gggaagagca cgatggccag ctctcccggg tgatctacat
    30781 cgacaagctc aaaccccggg tggggcgcat ggaaaaaatc ctggtgcagg agttcgacaa
    30841 aggacgcctg agccggatcc tctcctccgg gggtggggtt tgggccgacg gggtttggac
    30901 gctggaggga gggcaggtct tcgaggtgac tccggcggga gacgtggccc tgctcatgac
    30961 cttcgagcgc caaaaaatcc ccctggatct ctcccccgag gaaatcgagc ggtcggggcg
    31021 tgatccggat accatggggg tcttcgagat cctaaggcag atctctctgg tgtctttgca
    31081 gggaggggac gtgcatcccc tttggatttc cttccatact cgcctggcct ttccctgggc
    31141 cacggtggtg ctcgccctgg tgggagccac gctgggagtc cgtccccatc gcagcggcgc
    31201 ggggatcggc gtggggctca gcgtgctggt ggcctttgcc tactacgtgg tcatgtcctt
    31261 ctcccgggcc ctcgcccaag ggggaaatat ccccgtggtg gcgggggcgt ggctccctgt
    31321 ggcagtcttt ttggtgctgg gaggcttttt cgcccggagg gcgaacggat aggcccaggg
    31381 cgcggggatg aaagggcggg atggaagacg atgatgacgg cgcgaagcga agaaaccggg
    31441 gcgaaagctc cccttttgga gcggtgggcc ctggtggcgg ggggaggggc gttgccggag
    31501 gagatcgccc gccgatgggc gctccagggg cgtcgagggg tgatctacgt gctgggaggg
    31561 cgggccgagg tcctgcgtcc ctggggtgag gccacggtgg agctggatcg ggcagacctg
    31621 agaggcaccg tggaggacat gctccgccgg ggggtgcgaa aggtggtatt ggcggggacc
    31681 gtccccaaaa acaccatgta tcgcccctcc cagatggatg gcacgctgca agagctgctg
    31741 cgccgcctgc cggatagcaa cgaccacgcc ctgctggggg cggtggtggc ggccctggag
    31801 ggagcgggca tgacggtgat gcgctatggc gacgtcttgg gagaccttat ggcggaggaa
    31861 gggatcctgg gaggacggga gccttcgccg gaagagtggg gcgatgtggc ctatggcgtg
    31921 gaggtggctt cccgcctggt gcccctttcc ttcgggcaga ccgtggtggt gcgtcgtcgg
    31981 gcggtggtgg cggtggagtc catggagggt accgatggaa ccctggagcg ggcggggcgc
    32041 ctggcttccg ggggggtcgt ggtgaagctc atgaggggag accaggacga ccgctacgac
    32101 cttccggtgg tgggaccccg gacggtgcgc ctcatggggc gatggggcct cgggtgtttg
    32161 gcggtggaaa aggggcgcac gatcatcctg gaccgggaga aggtcatggc cttggcggag
    32221 gccgaaggga tcgccgtggt gggggtagcc ccccgccgga tcccggaggc ctaggccccc
    32281 atggggcccg aagggggttt catggtcgag gggatgcgct ctttcgtgct gagctgcgga
    32341 gaggcctcgg gggacgccta cgtggggctc ttggcccggg agctccgggc ccaggggttt
    32401 gccggggagc tctgggggat gtgcggcccc cggggggcgt cggcaggggt gactccccgg
    32461 tggagctttc aggagctcca gctcatgggc atttccgagg tgattcccgc cctgcccagg
    32521 cttttcaggc tccgagaaga gatgattcgt gccatctgcg aggcccgtcc cgggggagtg
    32581 gtgatggtgg actccccgga ctttcacctt cccctggccc gggggttgcg ccgccgggga
    32641 tacgagggca agctcttttc cctggccccc cccacggcgt gggcctggcg gccgggacgc
    32701 gtcaaaaccc tggagctttt ttccctggtc tttcccctgt ttccctttga ggatcgattc
    32761 tttcgttccc acggagtgcg gagcgccttt cgggggcacc ccctcctgga tgaaccctcc
    32821 ctggcgccca gcgaccgccg ggaccctccc cggcgagtgg ccctcctccc gggaagccgg
    32881 ccctcggagg tgcggcgcct gggagaggta ttggggggcg tgggacggcg gctgaaggag
    32941 ctggggatgg agccggagct ctcccttgcc ccttcgctgg aggcccccga ccgggagttc
    33001 ttgcgccggg cggcggaggg cctttccctg tcggaagacc ccgggccgga gctcatgaga
    33061 cgggccgacc tggtggtggg agcctgtggg accgctgcgg tggaggccct gctcctgcgc
    33121 cgattcatgg tggtgcttta ccgggggtcc tggagcagcc acctggccta tcgcctcctg
    33181 gtgcgcaccc cctttgtggc gctgcccaat ctgttgctgg atcgcccgat ttatcccgag
    33241 ctcctacaag gggaagtccg agaggaacgg atcgtgaaag aagtccttcg ctacgtcgaa
    33301 gatctctctt ttcgctcctc ggtggaccag gagctgcgaa gggggaggga gcggctcgga
    33361 gatcccgggg cgctggcctt ctgggctcgg gcggtgcggg aagagctcac atcttccgag
    33421 ggggtcgggg catgatcccc cccgcatcct cccccgtgct cctgctggtg aatggcccgg
    33481 gagaactgtg ggggtggggt cgccccctgg cggcggcgtt gaggcgtcgg gggctggagg
    33541 tgcgcctacg cctcttgccc tgtcagtttg cgggggggga ggagcgcctt ctcctggagc
    33601 gccttccgtc ggtgcgaggg gaaggtcccg aagggtacgc cgcctttttc cttcgagggg
    33661 gaacccctgc ggcggcggtg gtgcaattgg ggggagagct cggcgtgggg cggcgggtgg
    33721 cggagcgatg gaaggctccg ctggtctgct acacctacgg cttcaaacgg gggctcgaac
    33781 gctgcgatct ggtggccacg gcctttaatc ccatggcggc ggggctcaac ggggtgcgtt
    33841 gtgaagtggt gggagatcta gtggcggatg ccctgaccct ggaccggggc ccttcgccgt
    33901 ggggcgaagc cccttcccga gggaaaaatc actgccgttt ggcccttttt cccgggagtc
    33961 gccccgccat ccgtcgggag gcccggggat ttctgcaacg cctggtggag acccttcgtc
    34021 gggagggggg agagtttttc cccgtggcgg tgctttctcc ggcggtggac gacgtcgaag
    34081 cggagcagtg gcgagaagga ggcatgctcc cctggagggg gggggcgggg acggtgctcc
    34141 ccggggccca tatggccctg acccagcccg ggaccaacac tttggagctg cttcactgca
    34201 ccacgccggg gctggtcgtg gtccccgacg agtttttcga taaaatcccc ctgggagggg
    34261 tggcggggtt ggcaaaaagc ctcccgggat tgggatcttt cctccggcgc cgggccctgg
    34321 atcggtggcg gggaaagttc ctttcctggc ccaaccggct ggcggggggg gaaatccttt
    34381 gggaagcggt gggggccgtg acgccagaat ccctggcgcg ccgggtgcgg gaactttgga
    34441 ccgatgaggt gggacgggag aagatgacca gatctctgcg ggaactggcc gaggagatag
    34501 gcccgggggc tggggcggcg gaccgcctgg cggaaagaat cggagaaatg gcggcggggc
    34561 ggtgatgggc aaggccgggg gatcctaccg acggctattg gcccatgcct ggccctaccg
    34621 gcggaaggtc tacggcgcct tcggggccat gctcggggcg gcgatctgtg gcatgattcc
    34681 cccctggctc ctcaaggacg tggtggacga cgtactcata ggtcgcgagt tgggcatgct
    34741 caatttgttg gcggggggca tcgtggggct ttacctgggc aaggcggcct tttcctacgc
    34801 ccagcgttat ctcatgaccg aggtggggca gcgtctggtg ctagacctcc gcatccggct
    34861 cttcgaccat ctccaacgcc tttcgttggc cttcatcgcg gaacggcggg tgggggacct
    34921 tctgtcccgg atcaccaacg acgtggcggt gctccaggag gtggccacca cggccctggt
    34981 ggacctagtg gtgcagggcc tttccttctt gggcatggcg ggatttttgc tctatatcaa
    35041 ctggcgcctc accttggtga cctggaccat cctccccctg gcggcgtggt tcatcgaccg
    35101 atcgtccaag cggctccggg gggtgggaca cgaaatccag tcgtgtctgg cctctttggc
    35161 ggcgggggcc caggaggccc tggggtcttt gcgcatcgtg caggccttcg tcacggagga
    35221 agaggaactg gacaagtttc gccagcggag cgaggcaaac ttccgggccc tcatgcgggg
    35281 cacccacatt ctggctctcc tcgaaggggg cgtggaagtg gccctggtgg cggcgttggc
    35341 cttcattctc tggcttgggg ggcgcgatgt catcgagggc cgcctctccc cgggggagct
    35401 catcgccttt ttggggtacc tgggtttcat ggtccaaccc ctgagggtgg tgagccggag
    35461 cgtaagccgc atctcccaag ggctcgcctc ggcggaccgg atcttcgata tgctcgacca
    35521 gcccgcaggc ctggtccttc ccgcctcacc cttgtccccg gggcgccttc gaggggaaat
    35581 atctctggaa gacgtccact ttgcctacga tcccgcccgc cccgtattgc ggggtctctc
    35641 gcttggcatc gttccggggg aggtggtggc cctggtgggg gccacggggg cgggaaaatc
    35701 caccctggcg gatttggttc cccggtttta cgacccccag cggggggtgg tccgggtgga
    35761 tgggcacgat cttcgtacgc tgaatctccc cgagctgcgc cgtcagattg gcatcgtccc
    35821 ccaggacccc gtgctgctca agggaaccct tttctacaac atcgcctacg gctgtgcctg
    35881 ggcggacgag gggcgcgttc gggatgcggc gtgccaggcg gggattgccg atttcatcga
    35941 aacccttccc cggggctatg acaccgaggt gggggagcgg ggggccacgc tcagcggagg
    36001 acaacgtcag cgggtggcca tcgcccgggc cttggtgcgc gaccctcgga tcctcatcat
    36061 ggacgaggcc acggcctcgc tggacgcgga cgtggaggcc caaatccagg cggccctgcg
    36121 ccgggtcttt cgggggcgga ccgtgctggt catcgcccac cggctctcca cggtgcgcga
    36181 cgcccaccgg atcctggtat tggaccaggg gcgggtgacg gagcagggta cccacgagga
    36241 gctcttggcc cggcgggggg tataccatcg cctctactcg ctccagttcg gtgccgctgg
    36301 cggagggaag tgacccgtcg tgtccgaaag gaaagccgag agggtcctgc agagctttct
    36361 ggcccacgcc cggggcgaag aggccttcgt cggatggcac ctgctggcgc ccctgggatg
    36421 ggccgtgggg gcctgggccc ggtggaggaa tcgggggttt gaccacgggt ggtggctctc
    36481 cgaagagccc cttcttcccg tggtgagcgt gggtaacctc acgctgggag ggacgaataa
    36541 gacccccttc gtggccatgt tggtggagac cctggttcgg gcggggctcg cccccggggt
    36601 ggtgagccgg ggatatggag gacggactcg cactcccctt ttggtggaag gggggcgggg
    36661 ggatcgggcg cttacggggg atgaacccct actcctttcc gcccagctcc gcccctgtcc
    36721 ggtctgcgtg gcggcggacc ggcggcaggg ggtggccctg ttggcccagc gggggtgcga
    36781 cgtggtggtg gccgacgatg cttttcaaca ccgccgcatg gggcgcgatg tggacgtggt
    36841 cctggtggat gccgcctgcc cctggggtaa tggacggtac tttcctgcgg gcctgctccg
    36901 ggaaggagtg gagggccttg tccgatccca tgcggtggtg cttaccaagg tggatcaggt
    36961 attgcccgaa gtgttggaag atctggagcg ccgcctgaag agcatcgtgc ccgccgagag
    37021 gatcttccga tctcggctgg cctttggggg gtggttccgg gcccgggacg ggggatggga
    37081 gcccgacggg cccgactttt ccggaggggt attggccttt tccgccatcg ggagccctga
    37141 gagcttccgg cgcctcctgg agcgccaagg ggtgcgcctc cggggagagc ggatctttcg
    37201 agaccaccac cactactccc cggaggacgt gatggctctg gagcggttgg cccgaaccct
    37261 gggggtgcga tccctggtct gcaccgaaaa ggacgtgtac aacctgcccc ttagcccggg
    37321 gggatccccc ttttgtcccg atgataaacc ccccccccag gaggatttgt cggtgtggtt
    37381 tccccgggtg cgcaccgtgc tggatgatcc cgggcggttc tggaagaccc tggggcaggg
    37441 gttggtgccc cgcatggtgg tggcttccaa cggcttcggg gaggacgcca tgggaagcct
    37501 gctggcggac aagctacggc gccgttttcc cgggggaagg gtggaggcct tttccttggt
    37561 ggggcggggt caggcctatg ccgaacggaa aatccccgtt ctttctcctc cggcggagac
    37621 ccccagcggc ggagtggtga agtatagctg gggtgacttc tggcgagacc tgcgcagcgg
    37681 tctcctgggg caggtacgag ggcagcttcg agcctgggag gattaccggg ggagggcgcg
    37741 caccgtgctc tgtgtgggag atgtctatct tctcctgcat accctctggg ggcaggggca
    37801 ggcccccctg ctggtggcca ccgctaaaag cgagcttttg ggggggcatt ggcacctgga
    37861 gcgcctggtg ttgcggcatc ggtgccgcat ggtttggacc cgggacgggg ccacggcgga
    37921 ggagctccgg cgccacgggg tgccggcggc cttttgtggt aatcccatca tggatctggc
    37981 ggaagaagac atccagaaaa tccctttgtc gggggataat atccgtggac cgggaggaaa
    38041 gggagccccc cctcgggtac tcctcctccc cggcagccgg ggaagggcct atgacgactt
    38101 ggcgttgctc ctggacgcgg cggtgaaaat ttgggagcgc caggcggtct ctttcctatt
    38161 ggttttggcc cccaccctgg aacgggggcg cttcgccgaa gcggcgctcc ggaggggatg
    38221 gcgcctggag ggagaaaccc tcctggggcc atcgggcctt cgggtgcgag tgaccgccga
    38281 tcccgtggcg cggggggcga agggagccca ggtggtcctg gggctcggag ggacggcgaa
    38341 tcaggtctgc gcggggctgg gagtgccggt ggtttccgtg ctggaaaagg gaaagctggt
    38401 gcaaaaaaaa ctcttgggag aggcggaagt gctggtcccc cccgaggcgg acgccctggc
    38461 ccaagaggtg gacgccctgc tccgggacga ggcccggcgg tgtgccatgg gtcgggcggg
    38521 ggcccgggcc ttagggggag gaggggccct ggacgcggtg gtgtcccacg tggcgtccgt
    38581 gttaggatgg gagggacgct gtgccgtggc ggagcgcctg aacgcctttc tggcctcctg
    38641 ggggccggag actccggggg cttccggagg gggatggggc gcgccggagg gccccgccgg
    38701 gacggtcccg ggggacgcga aggagatgaa gatatgagca cgttggcggt cattcccgcc
    38761 cggtatgcga gcactcgcct tccggggaag cctctggtgc cggtggcggg agttcctttg
    38821 gtggtacggg tgctacggaa cgtccagcgc tgtcgccggg tagacgcagt ggtggtggcc
    38881 acggactccc ccgaggtgcg cctcgtggtg gaggccgaag ggggagtcgt ggagatgacg
    38941 cccccggagc tccccagcgg aggagatcgg gtggcctggg tggcccggcg tcggagcgag
    39001 gacgtggtgc ttaacatcca agtggacgac cccttggtgg ggcccgacat ggtggacccc
    39061 ttggtggagg ccctggaagc ggacgaagcc atccagcttg cggtattggc caagcgcatc
    39121 gaacgcatcg aagaagtccc cgacccccac atcgtgaaga tggtcttcga cggccagggg
    39181 aaggcccttt atttcagccg atctcccatc ccctatccgc gccatccggg aggggtctgg
    39241 ttcaagcaca taggccccta cgcctatcgg cggaactttt tgctggattt tgcctcctgg
    39301 gagcccacgc ccctggagcg catcgagagc ctggagatgc ttcgggtgtt ggaacggggg
    39361 catgccatcc gctgcgtccc cgtggaacgg gacaccatcg aaatagacac tccggcggac
    39421 gtggaggcct gcgagcgcca tttggcgcgc ctcgcagagg aaggggatca ccatggagcg
    39481 tagcgtacgg gtgggcgatg tgaccgtggg ggagggaacc ctggccatct ttgcggggcc
    39541 ctgcgtcttg gagagtctgg agatggccct ggaggtaggg gaggcctgtc gtgatctctg
    39601 ccgatccctg gggctggggt acgtcttcaa ggcttccttc gacaaggcga accgcacgtc
    39661 cctttcgagc ttccgggggc cggggctgga gcggggcatc gactggctcc gggagattcg
    39721 ggatcgcctg gaggtccccg tgctcacgga catccacgag gcgtggcagg cggggcccgt
    39781 ggcggagtcc gtggatttgc tccagatccc cgcctttctc tgccgacaga ccgaccttct
    39841 cctggcggcg gcccagacgg gcaaaccggt gaacgtcaag aaagcccagt ttctggcccc
    39901 ccaggacatg gctcccgtgg tggaaaagct ccggggggga gggtgccggg agatcctcct
    39961 ctgcgagcgg ggcacgtcct tcggctatca ccagttggtg gtggacatgc gctccctgcc
    40021 catcatgagg gacctggggt gccccgtggt ctttgacgcc acccacagcg tgcagatgcc
    40081 gggagggcag ggggattgca gcggcggaga ccggcgtttt gtcctacccc tggcccgatg
    40141 tgccgtggcg gcgggggtcg atgccctctt catggaaacc catccctgtc cggagaaggc
    40201 cctttcggac ggtcccaacg tggtgccttt gaaccggatg gagcgggttc tttcggaggt
    40261 ggcggcgcta tttcgagcgg tccaacccct gggtcccgcc gacttggcat ggtggcaatg
    40321 agctgcccca tgcttccccg agaccgggac ctctgccccg tggagggcga ggccctgtta
    40381 gagaccgggc gtcgggtgct tcgccaggaa ggagaagaac tcctccgggc cgctgggcgg
    40441 gtgggagatg agatggtgga ggccgcccgg cgcatcgccg cctgcgacgg ccgcttggtg
    40501 gtgagtggcc tgggcaagtc gggactcata ggccggaaga tcgccgccac cttcgcctcg
    40561 ttggggaccc cgtctttttt cctccacgcc acggagggat ctcacggcga tctgggcatg
    40621 gtctgccggg gggacgtggg ccttttcctg agcaacagcg gtgaaacccg ggaggtgctg
    40681 gagctcctcc ccttcttccg gcgcatcggg gcccccatca ttgccatgac ggggaaggaa
    40741 tcctcccgtc tggcccaaga agcggacgtg gtgctccttg ccgccgtgga gcgcgaggcg
    40801 gatcccttga acctggctcc caccagtagc accacgctcc aattggccct gggagacgcc
    40861 ctggcgggca tggtgacgga gctccggggg ttgtgccagg aggacttcgc cctcttccac
    40921 cccggcggag ctctggggag aaagctcctg ctccgggtgg aagacctcat gggatcggga
    40981 gagcgcctgc cccgggtgga gcgaggggcc acggtgagtg aggccctttt tgaaatgacc
    41041 agtaaagggt atggggccac ggcggtgacc gacgaagaag gatttctggt ggggattttc
    41101 accgatggag acctgcggcg ctttctggag cgatcccttc acccttccat ggacgccccc
    41161 atcgaagaag ccatgacgct gtccccgaag accatagcgc cggaccgcct cgccgccgag
    41221 gcggtgctcc tcatggagcg ccatgaagtc tccgtcctca tggcggtgcg ccaggggaag
    41281 cccgtgggga tcatccacct gcacgaactc ctcaaagcgg gcctggcttg acatggtcga
    41341 ggggcggggc ctaggggagc cgtggtggct ggggccttac ggtctgctgg cccggggggt
    41401 ccacgccgcc tgggaacccc gtctccggcg gcggtacggt gaagggtacg acgagcggcg
    41461 gggagagctg ggaaaggatc tgagggagct agggaaggcc catccccttt ggatccacgc
    41521 cgtctccgtg ggagaggtcc aggccgccgc ccctttggta cggatcgtca aggaagagag
    41581 ccagggaggg atccccgtgg tcctttccac catcaccccc acgggacggg ccatggctcg
    41641 caccctcttg gaggggtacc ttgatgccct gatatactac ccctgggacg tgccgacctt
    41701 tgtggaaagg gccgtggagg ccctctctcc ccggggctat gtggtgatgg agaccgagat
    41761 ctggcccacc ctgctcttcg ccctccggcg gcgccgaatc ccgacctttc tggccaacgg
    41821 ccgcctttcg ccccgcacct ttgaccgcgc tcgacgatgg agctttgtgt ggaaggacgt
    41881 gtggggttct tttgagggcc ttttcatgcg atccgaggaa gatcgggccc gcatcgtggc
    41941 gttgggggcc cccggagagc gggtgcaagt cctgggagat tgtaaggtgg atgccctttt
    42001 gcaccgccgt ggggcccccg agaccctttc ggtcgccccg ccatgcgacg gtccatttgt
    42061 ccttgccggg agcacccacg ccggagaaga gaccgtggcg ctggaggcct ttcgccagct
    42121 ccgaggggag tgcccctcgc tgcgattgat cctggtgccc cgccacccgg agcgggggag
    42181 agaggtgtgt caagaggccc agctgacggg gccagcgggg cttttttccg aaaaccccgg
    42241 gagttggact atactggtgg tggatcgggt gggggtgctt ttttccctat accgatgggc
    42301 ccggggggcc ttcgtggggg gaagcctggt gccccggggg ggacagaacc tcctggagcc
    42361 cgccgcctgg ggggtgcccg tggtgcgagg gccccacatg gaggactttg ccgaaggggc
    42421 caaggccctg gaattcctgg gggcggctcg gacggtgaca gatgcgtcga gcctggcgga
    42481 ggcctggcga tggacgctgt cggacgaggg aagggacgcc gggcttcgag gaagggatta
    42541 cgtggaaggc ttgggcgggg cggcgcgggc gacgtggcgc gcgatgtctc ctcggtggga
    42601 gaagatgtag gggggagggt gtttttcccc ccgggaaaaa agagaaggag cgtggtggat
    42661 cgtgaacgcg caaatgtttt tggcggggcg gacccagttt ggccccggcg tcgtttccaa
    42721 agtgggagag ttggccaaat cccttcgggc ggagagggcg gtgttggtca ccgacggcag
    42781 catggaacgc ctgggggtgg cggatcgcgt cgagaaactg atccacgaag cgggggtgga
    42841 gtgctgcgtc ttctccggtg tggaagccga accatcggtg gagaccacgg atgcggtggc
    42901 ggagctggcc cgggagcatg actgccagct ggtggtgggc ctggggggag gaagctgcct
    42961 ggatgtggcc aaggccgtga gcgtgctcat caccaacatg ggctcggcgg cccagtacca
    43021 gggcatgaac ctcgtgaagg agcgcggggt gccaaagatc ctcatcccca ccacggcggg
    43081 tacgggatcc gaggtgacct tcacggcggt gctcatccgc aagagcgacg gcttcaaggg
    43141 gggcataaac gacgagaagc tcttctcgga ctacaacctc ttggatcccg aactcaccct
    43201 ctccatgccc ccggaggtga cggcggccac ggggatggat gcgctgaccc acgccctgga
    43261 ggcctacacg gggcgcgccg cctcggtgtt tagcgacatt ttcgccgaag aagccctgcg
    43321 gcgtattggg cgctatctcc gggtggccac ttggaacggc aaggacctgc acgcccggag
    43381 cgaaatgctt ttggcctcgt acttcggcgg cgtggcgctg gccaacgccg gggtgggggc
    43441 ctgccattcc ctggcgtatc ccctggggag cgtcttcggc gtgggacacg gcatggccaa
    43501 cgccctgctg gtgccctatg tgacagccca taacgccctg gcagccccgg agaagtacgc
    43561 ccaggcggcg gctctgatgg gatttggggg cgacggcctc tcgctgaggg atcaggcgtt
    43621 catctgcgcc gatggcctgg aggaattggt ggaggatctg ggccttccta ggacgtttaa
    43681 agatctcaag agacgtaaga tcatcaagga ggagatctcg gtggatctcc tgccggagct
    43741 ggccaccaag gccatggcgg tggctcgccc catggagaac aatccccgct tcatgacgga
    43801 ggaaagttgt cggaacctgt atcgggaggc gatggaataa tgccgggttt tgaactcttt
    43861 gaccaacaag aaattgatgc cttggcggac gtgatccgcc gaaagatggt gcaccgctat
    43921 tcttttgtga actgtcggga cgaaatctac cgggtggagg agtttgagaa cgccgtggcg
    43981 gcgaagttcg gcgtcaagcg ttgccaggcg gtatccagtg gcacggcgtc gctctacgtg
    44041 gcgctgaagg ccatgggggt ggggccgggg gacgagatca tcaccacccc cttcaccttc
    44101 atcgccagcg tggaggccat cctggagtgc ggggccaccc ccgtgctggc cgaaatcgac
    44161 gaaagcctaa atctggatcc ttcggtgttg gaagagtaca tcactgaccg gacccggggc
    44221 atcatgcccg tgcacatgtt tggtgcggca gcggatttgg atcgcatcca agaagtgtgc
    44281 acggagtacg gcgtccccct gctggaagac agctgtcagg ccatgggggc cacatatcgg
    44341 ggcaaggccc tgggtaccat gggtctctgg ggtacctaca gccttgatcc ctacaagatc
    44401 ctcaccgtgg gagaaggggg gctcgtggtc accaacaacg aagagctcta ccgccgcatg
    44461 gagtactatg cggaccacgg ccatatccat agcaagacca ttgatcgagg agcagaagag
    44521 aaggcctgcc tggggttcaa cttccgcctg agcgagctcc agggcgccct ggggctggta
    44581 cagctcgcca agtttgacga cgccgtggcg cgcctccgga gcaacaaaaa gcgcatcctg
    44641 gagagggtgg gaaatgtgga gggcctttcc ctgcgggacc tccccgatcg cagcggagaa
    44701 atagctaccc agctggtctt cctgcttccc gacgccgccc gggccaaggt ctttcagaaa
    44761 gtatcgaaag aggcgggggt gggatgcgcc cttctgtctg aaaatacctg gcactacgcc
    44821 cggcactggc agaccctccg agatggggcg tcctgggccc gggtacgttg ccccttcgac
    44881 tgcccccatg cccccgagac tcctcccctc taccgtcccg tggagtggcc caagacccat
    44941 ggaatcctga gccgggccct gtgctacggc atcgacatct gcatgcccga cgaacggatc
    45001 gaaaaaaccg tggcggccat tcgggcggcg gcccgggccg cgctttagac aggccctttt
    45061 acgggcgccc tttgcccact agaaccagca aaagctgagc cctcgggatc gtttcgggga
    45121 aaaaagcgcc ggagagcggg gacgttctcc ggcgctttcg aagtaaaaaa cgggaaaggg
    45181 ggggcgggcg ttgtccgaaa gctccatcca gggagtgggc gtgctcagcg atggcctccg
    45241 gggacacctg tcccagagcc ggggcgtggc ggagtggctt aggcgggact ttggcgtgga
    45301 gggaaaagag ttcgacgtgc cccgactggt gggatggcgc cgatggcagg cgctaaagct
    45361 ccgggccctg acgttgcgcc ggggaaatcc ggccacggca ctccggtggc tcgccgcctc
    45421 ctcggggatt cctctgcttg agagcgtgaa aatctgggcc caagaccaag gcatggacct
    45481 ccggcgcatc ctcttccttt cggccggaag cgcggccgcc cctttttgcc tggcgctggc
    45541 ccgacaccta ggagcccggt gcgtcactct catgactccg tcggtgctcg gcaccgcccc
    45601 ctttgatgcc gccgtggtcc cccggcacga cgcccccgtc ccttctccct ggaccttggt
    45661 caccctgggg gcccccaacg ccatcgtccc ccaggccctg gccgaagcgg gggaggaact
    45721 tctgcggcgc ttccccgccg gggggcccga aggagaaggg gaagtttggg agcgttgggc
    45781 ggtgctcctc ggaggggacg acgggaatta ccgcctttct ccccgttgga tggagcggag
    45841 tgtgggggcg ctcttatctg cggcgaaagc ccggggagcg gtcctttaca tcaccacctc
    45901 ccggcgcacc tcgacggagg ccgaagaggc cctgcttagg agcgtggcgt catcgtccgc
    45961 cgtccgcctg tgcctcctgg cttcccggga tccctggaac cctgtccccg gcctcttggg
    46021 cgcggcccac cgggtctttt gcaccgaaga ctcggtgtcc atggtctccg aggccctttc
    46081 ggggggacat cgcctggggc tcctgagggt ggagcgccgg gggggcctcc ggggatggtg
    46141 gggccacgcc gggacgaaac tcgtccgttg gggcgtctgg ggagagggag ccctgggggg
    46201 ggcaattcgt ttcgacgccc tcttcgagga gttctttcgc cggggttatg ccgtggagtg
    46261 tggccccgat cccgaggcct ggccctggga ccgggtgggg cgcttcgata cccctccccc
    46321 caatgaggcc cgccgggcgg cggcctggtt gatggggctg tggccatgac tgcagcccca
    46381 tcggcttcgt taaggggatg accggtcggc ccccttcgat ttttccccgg ggcgatcggt
    46441 gagggggccc cccctagacc tccgggaact ccttccggaa agaatttttt tgcgcgaaaa
    46501 ggggggagat ggctataatg gcgcggaacg gaacgagcac cggaagcgac gaggggccta
    46561 tcccccccga gacgttttcg cccggcccgc cgaaatgaga gggaggaaga gccatgggtt
    46621 acgatttttc cgctgtggag gccaagtggc agtcttactg ggaacgtaaa ggggtttttc
    46681 ataccgaagc ccacgaggaa aagccgaagt tctactgcct ggaaatgttt ccctacccca
    46741 gtggggcgct gcacatgggg cacctgcgga attactccat cggggacctg ttgagcaggt
    46801 atctttttat gaacggctat aacgtgttgc accccatggg cttcgacgcc tttggcctcc
    46861 cggcggagaa cgccgccatc aagttcggcg tgcaccccaa cgcctggacc tggaagaaca
    46921 tagagcacat gaccgatcaa ctcaagcaca tggggtgcag ctacgactgg cgccgcaggg
    46981 tggagacctg tcacccggat tactaccgtt ggacccagtg gctcttcctg cagttttaca
    47041 aaaaaggcct ggtgtatcgc aagcatgccc aggtgaactg gtgtcccgac tgtgccacgg
    47101 tccttgccaa cgagcaggtc atcgacggag gacattgctg gaggtgccat tccctcgtga
    47161 ccaagcgggg gctggagcag tggtttttcc gcattacggc ctacgcccag gagcttttgg
    47221 aaagcctgga tcggctcccg gggtggcccg accgggtgcg gatcatgcag gcccactgga
    47281 tcggtcgctc cgagggagtt cgcctctctt ttacccttaa gggcaccgaa gagcgcatcg
    47341 agacctttac cacgcgcttc gacaccatct acggcgtcac cttcctggca ctggccccgg
    47401 agcaccccct ggtggaacgc ctcgccgcca cgtcccccga aggagatcga attcgagagt
    47461 ttgtccaatc ggtgatatcc gccagcgaga tcgaacgcac cgccctggga ggggagaaga
    47521 agggcctttt caccggccac tatgccataa accccgtgaa cggcgagcaa gtgcccatct
    47581 ggatcgccaa ctacatcctc atggattacg gcaccggagc catcatgggc gttccggccc
    47641 acgaccagcg ggacttcgag ttcgcccgaa agttccatat ccccgtgcgg gtggtcatcg
    47701 cccccgccga cggctcctcg ttggacgggg cctccatgga tcgggccttt gatgaaaatg
    47761 gcattcaggt aagcagcggc gacttcgacg gtctgcccac ggcggaggcc attccccgca
    47821 tgaccgcctg ggcggaggag caggggctgg gtacccgaga ggtgaactac cgcctccgag
    47881 attggctcat ctctcgccaa cgcttctggg gggcccccat ccccatggtc tactgcgatc
    47941 gctgcggcgt ggtgcccatc cccgaagaag agcttcccgt ggtgctgccc acggatgtca
    48001 aacttctgga cggagggaga agtcccttgc cggaggcgga tcactggaaa aacacccgtt
    48061 gcccctcctg cggtggggca gcggtgcgcg agacggacac catggacacc ttcatctgct
    48121 cgtcctggta cttcctgcgc tacacctccc cttggacgaa gcaagctccc ttcttgaaag
    48181 aagacgtgga ctactggatg gacgtggatc agtacatcgg cggcattgag cacgcttgtc
    48241 tccatctcat ttacgctaga tttttcacca aggccctggc ggatatgggg tatctgtcgg
    48301 tgcgggagcc cttccgcaac ctcctcaccc agggcatggt cataaaagat ggggccaaga
    48361 tgtccaagtc caaggggaac gtggtggatc ccgacgagat catcaaaaag tacggcgcgg
    48421 acaccgcccg gcttttcatt ctcttcgccg cccctccgga gcgggatttg gactggtccg
    48481 accagggagt ggagggatcc catcgattcc tgaatcgggt ctggcggctg gtggaagatc
    48541 ggctttcaga gcttcaaagt gccccggcgg agggcccttc cctggaagct ttgggggatc
    48601 gagcctcccg ggatctgaaa cgggtcatcc accgcaccat tgaccgggtg actcgggaca
    48661 tcgaagaaga gcgccagttc aacaccgccg tggcccgcct catggagttg gccaacgccc
    48721 tgggggacta tcgtcccgcc gatgcgaccc agagagcgct tttccgggaa ggagtggaga
    48781 ccctcctgac gtgcctttct cccttcacgc cccatatctg cgaggagcta tgggccatga
    48841 cggggcacga ggagtgcctg gcccggggga tctggcccca ggcccagcca gaggccttgg
    48901 tggaagaggc ggtgaccgtc gtattccaag aaaatggcaa ggtgcgggag cgcctggaag
    48961 tccccgccgg actatcggcc gaagagctac agcagcgagt gttgaatcac cccgccgtac
    49021 gagatcggct ggagggcaaa aacgtcatca aggtggtggc ggtgccagga aaactcgtga
    49081 acgtggtggt gaaggcgtaa gtgcctcatc tgcacgtggt ggacgccggg gggagctccc
    49141 agcgacgcct catctccgag gcgctggatc gccatggccg ggaagggtat gcctgcgagg
    49201 ggcgttttga gcacggcgat cgcccctggg gagagctctt tgcctctttc ggtggggcga
    49261 gcctcttcga gaaaaaaagc gtcctggtgt tggaaaaggc gagcgccttg ggtccctttc
    49321 ccccggagct tgagccttcc ttggagcccc cggggtcccc tacggttttt ttggcggttt
    49381 acgacgggga cggccgaaag ttcctttcct ccgaggcctt ggcccggtgc accgtggatc
    49441 gggtggaggc ccctccccga tggggcgcgg cgcgaaagcg ttggatcgag gccctcgcgg
    49501 ccgaggaggg cgcccgactt tccgcagagg ccggggccct cttaggggag tggtacgagg
    49561 atcccgagga gctacggcag gagatcgcca agctcgccat ggcttcccgg ggaaatgagg
    49621 tggggggggc gctggtgcgc tccctttgcg tcgacgaggg gcacggtttg gtactctccc
    49681 tgctggatgg ggtgtgccga aaaaatcgct ccgaggtgat gaaaacgctg gggctcttgc
    49741 gccgctccga agagctcctg cctgtgggca cggccctcta caatcgcctg cacatcgcct
    49801 ttttggccac gtgcttcacc ggcgccgatc tggaaaagat ctatgcggct cagggggtgc
    49861 gggattacgc ccggcgcctg gcggcggagg cgggacgcca ttatcctccc cgggcgatcc
    49921 agagagcggt ggcggaactt atcgccctgc tctgcgccga aaagtgggga aaaggcgcgg
    49981 ggtgggtggg gatggacatg gttctcctgc gcttactgga cgcggggagg ggatgacggg
    50041 gagccacggc gggggccacg gccaaagaag ggcccggagg atggctgggt cccggggagc
    50101 ccttctagaa gcctaggggc ctccccgagg atcccggggg gaatggcgcg agaaaggggt
    50161 gggagcctcg cgacccccac ccctgggaac gtccatcttt cgaaaaattc gaggcctttg
    50221 accgcagaag acgggaagct acgcctggag cgtgagggac ttcacgagcc tggccagacg
    50281 cgccttgcgc cgggcggcgg tgttgcgctg catgacgccc ttcaccacgg ccttgtccaa
    50341 gacggactgg gcctcgttca ggcggttttg agccacttcc gcgtttccac cggccacggc
    50401 ttccatgacg cgcttgccca cggtcttgca tcggctgctc cagaagcggt tgtagacccg
    50461 gtttcgctcg gcggtgatca cgcgggattt ggcggacttc ttgttcggca ctcctttcac
    50521 ctccctttat cacaagcggg tctcattcta gcacaaggcc cgtcttcgac gcaagagaac
    50581 ttttcccgag cgcgcgtcgt gcgatgccct ccgtcgcgcc ctcttttcct ctggatcgtt
    50641 gtatgggata atcggtgccg cattactgcg gtgacctttt tcctgacaga taaggaccgc
    50701 tcttgagggg gaaaagccat cgacgcgaag gaggagatct ccatgacgag aaacggcaag
    50761 ggcgtggggc gtttgtggcg agggtggttc ctgttggaag tgtttttgat tttgggggga
    50821 gcccatggag ctctggggta cacggtggga cagggactcc tttccgtgga gcgggcggaa
    50881 cagcaggctc gaagcgttcg aggagtgctc caggaataca tggaccgcac ggggaaatgc
    50941 gtttccaccg tggataagat ggggtacggg ggattggcaa atctgaaaga cgaagagggg
    51001 acgttgcaca tgaccccccc ccgatcgggg tttgaggtgc ccctctatct ccggatcaag
    51061 gcccatctgg ttcaaaatcc cggcgccaaa atctccctgg aggacatgtt ccgcatggga
    51121 ctggagtcct gtggatcggg gggaggggag gtcaacttag gagacgtgtt gctgtccatc
    51181 cacaacgtgg tgcggatcct cgcccggccg gagaactgga cggaaagcga cgcggacaaa
    51241 ccaggatggc ggaagatccg ggatatgaag gatccggcct gggatattat tcaggatgtc
    51301 tgcggcgtcc gttctgcggg agaaggaaaa accttggcag agatcttggg gatgcggcgc
    51361 atgtcagatc tggcggccct atcgccggag aaggaggaaa agatccgcct tctccaggaa
    51421 aagcgccgtc gcgatctgga agcggcggag gctcggatgg ccgccgtgga gaaagagctg
    51481 gaaatcctgc gccagagcat tccctatacg ccctacgcca cggtgcttcc cgaaagctcc
    51541 atggagatta tccgaaaaat tgaagccctg agcggtgaac gcagggccct ggattaccaa
    51601 atcgccaact tgcgcgaaaa ccccacgaac aatctggtgg accgccagat cgggtttacg
    51661 ctctttgatc ccgccggggg gatcttccag ccccttccgg gggcggagga gtgggtggga
    51721 aacggaggaa accactacta tttctggctg gggagcctga taaacctcgt cggaggaggg
    51781 aagtccgccc tgggggcgat gggctacgag aggcttcaga aatacctggg ctccaacgag
    51841 gaatacgccc ggggtctcgt gcagctttcc cacttcgtgg cgggatccaa gctggggagc
    51901 ggcgcctacg gcatagcccg acgctgtgaa ctgcgccgct tggtggctcg ttctcgttcc
    51961 atatcgggca tgcgcctcat tcccggcatt caaggagggc ccggggggga taatccggac
    52021 gacaatgaga acatcggggc ggcccttctt cccctggcac ccttgccccg gaagtttctg
    52081 gcgctctgcg cggaaaaaga accctctctg agaccacttt ttcagggagg ggagaaagaa
    52141 gagctggagc gcgccctgga tccgggcata ttagggttct gggcggaacg gggagaatct
    52201 gtcgaggttt ttcccggaga tcgaagcgcg gagcccctgg cggaggcgct ggcgttcatt
    52261 ccttccgtgg cggtgctgaa cgatggctat gccgagggag cagtggccct gctggcccgg
    52321 cggggagagc cctcggtgct tttgtcgccc cgagtgactc cggaggtgtt ggcccgttat
    52381 gtcgtgctgg tgatccccag cggggcgctg gcggagtgga gttcctcggg agccctgcgg
    52441 aaaaagctcg aaacctatgt ggcgggaggg gggcgcatcc tgacgctctc ccagcaactt
    52501 ggacggcact acggcgccct tcccggtgcg ccttcggcct atggctgggc tgaggaccag
    52561 agctgccagt tcggttctgt ggccctcacc gcgtcggtcc ccgccttcgc ttccctgacc
    52621 cgggagaaac cccacctgaa cgtggacggg tacttcctgg attaccctcc ggcggcggag
    52681 gtgctcctag cccgaaccaa gaacgaccag ccctgtatga tttcctacgc cgtgggacgc
    52741 ggtggggtcg tcgccacgaa cctctatctg gactgggctg cggcgaacca tcaggggacc
    52801 gaagacgaag ggctgatctt tcgggatctg gtggcctggc tcgccagtgg ggccggagag
    52861 ggggaaagtc tccccttggg agctcccgaa agccccatgg ttctgaagcg ggagatccgt
    52921 tacgaggccc cttcctcccg cgacacggcc ctggccctct tgccgaccct tttgttcctt
    52981 cccgccgggt cgtcggatct tcgagagcaa gtcggggctt ccgaaggaga gaaggacctc
    53041 tggagggcgg aggaggggcg tctggtgctc ctggatcccg acggaaacct tcttgaggga
    53101 gagctctttt cctgcgacct ttcccccggc gagacccggg agctttccct gaagaccccg
    53161 atcttgaaag ccccggggtt ctatcggggg tttctggagc ttcgggggcg tgggggggcg
    53221 gatcttgctt ccctgccctt ggggtgggtg gccgccgtgg gagtgcttcc ggccctggcc
    53281 agtggagagc gtcgagatct gaatttttcg gcgcagagtg acctggagaa ctacgtccgg
    53341 ggaggggtgg ggaagttctc gctggtggca tggaaccggg ggaacgccct ccggcggctc
    53401 cgggtggact accgcctccc acacaatctt tcttacgcca aggaccccac cccctaccag
    53461 gggagcattt cccttgacgt cccccccgga gggcagggaa gttacgagtt ttcctgcccg
    53521 ctggtgaacg ccgacggcat cgaccggctc tgggcgacgt tcttcgacgg tgtcacgggg
    53581 gagaacctgg gcaccgtctc taagggattt tacaccagga cggcctcctc cgtcgtccgg
    53641 ctctctccgg acaaggaaag cgtcgaggcc ggagctctcc tgcacgtggc gcttcgggcg
    53701 ggaaatccct ttgaccagag ctcctccggg cgcctcgcct acgccgtcac cgatgcggcg
    53761 gggacgagcc acgccctcaa agagcaggac gtttccttta cttcggaagg atacgaggga
    53821 gtcgtgagcc ttgacgtgcc ggaggagatc ttttccggtc caggggtgct ccaagccatg
    53881 gtgaccatcg ggggctccat ggtggggatt ggcgaagcgc cgctggaatt ggtggggccc
    53941 ccggtgcccc tttccgggca ggtgagggat cggttctcca aagcagctct ttcgggggct
    54001 tcgctgacgt tttattttgg ttcccgcctc ttcgaggccg tgacggatgg cgagggaaag
    54061 ttctctctcc accttcccgg cgcggcctat aaactggagg tccgcaggga agggtacgtc
    54121 cgggtcgccc gggagcatct ggtctatccg gaagccaatc cgcccctgga catactgctt
    54181 cttcccgagg gggcggcggc gggcatgggc accgtggagg gcacctgtcg ggaccgggtc
    54241 accggagaac ccctgggcgg ggtaaccggg cgcttcgagg gagggggcga ggtctacgac
    54301 gtgttgacgg acggaggagg gcgcttttcg accaccctgc ctccagggga gtacggcgtc
    54361 accatctggt ggggcggggc gttgatatcg gaccgttttc ctctgcgggt ccttgaggga
    54421 tggcgccagg tccttgacgt ctacggcgcg gtggggaaat tttctccgga ggtccgggac
    54481 ctgatgacgg aagctgtcct ggaggactgg cgcatggaga tcttccgtcc gggggacgag
    54541 aaaagccgca gggatgtttc cgccgccgcc cggggcggag aggtgcgcac ccccggcggt
    54601 ggccgatggg gtttccacct ggagaaggag gggtacgccc cgctgaatac ggaggttttt
    54661 atcagcgaac gttccggagc atatccgttt tttctgaaac ccaaggcctt ccctctggag
    54721 atcgctgtgc gagatcttct cacgggggat cccttggaag gggtggaggt gcgtctggat
    54781 cgccccggcg gagctccggg accccggggg aacaccttgg gggacgggaa ggtctccctg
    54841 gtctctgaag aggggcgttt cacgctcacc ctggggcgtc agggatatgg gcccgtcgtg
    54901 acggagctct tcgccgggcc ccagggggga ggctccgggg agtactggat gcgccccgag
    54961 gccacgccct ggaccctgga agtcctggat atccgggatg gactccctct ggagggggtg
    55021 aggatgcgcc tggcccgccc cgacggcgcc gagccccagg aggggatcac ggaccgtcag
    55081 gggcagtgtg gcgtctctct gaaagacgga cgttggacgg tgcggttgga gcgggaagga
    55141 tttaccacgc tggagaccga aacatggctt tccgccgcca ctccggcggt acgggaacgg
    55201 cgcggggcgg agcgctacta cctcgccccc gatcccgccc ttccccaggg aacgctgacg
    55261 ttgacggtgc aggacgaata cgccgccatg cctctggagg gggtgacggt gaccgcccgg
    55321 catcctgggg gaagggaagc gatggggcgc accgatgcgg cggggcgcgt gtccctggcg
    55381 cttcttgatg gccgagaggc cttccatctg gaaaaggaag ggtacgaacc cctggacacg
    55441 gagctcttct tcagccaccg gggggcctcg gaagagcccc tctggctcac tccgacccgg
    55501 gcgtcccgga ctgtgctctt ccgggatctc cagaccgaag ctcttctgga cggcgtggaa
    55561 acctgggccc tagagggaga gagggtcgac cttctgggac gatcgggacc ggaaggaacc
    55621 ttggcggtgg accttcccgg aggacggcgt cttttccgct ttgcccggga gggatatgag
    55681 accttggaga cggagttctt ctgggctccg gggcccgtgg ggcgggagga tatcgaaacg
    55741 gtctacctca ccccccggag ccgtcctttc cggggccggg tgcgggatct cggcggcacc
    55801 cccctggcgg gggtctcggt gacggccctc tggggagaaa ccccccggag cacttccacg
    55861 gcatccgacg gcaccttcat tatggacctc cctcgggggg tgggggcctg gagtttttcc
    55921 gcccccgggt tcaaagaggg acacgcgcag atgtactggg ggctgcgtag cgaagaggtc
    55981 cccgattttg tgctccttcg tccggaagag aatccctcgg gggaaggaga ggtccacatg
    56041 aaggttgtgg acgccgtcac cggggcgccc ctgaccgctt tttcggccca catgggggcc
    56101 cggggttgga tggagatggg ggtgggcgcc gccctggtct ccctgcctgc ggggaagcac
    56161 tccttcctgg tgcaggctcc gggatatctg cccacggggt ggtttgagga aagcgttgtg
    56221 gccggaatcc gtctggggcg caccgtggcc ctcatgccca taacaggaga gctcgttttt
    56281 cacgttcggg acgccctcac gggggatccc ctgccgcgtt tcaccgccca tctcgtggat
    56341 acggggtggc gcgaggggga gcaggggaga atcacggccc ggcaggacgg ccggaaccgg
    56401 gacactcttg tgaaggccga cggatacttt ggaacgggat ggtttcttcc ccgggccttt
    56461 cctgggcgga gcgtggagcg caccgtggct ctgcgacccg tcgtggggga gttggtgttg
    56521 cacgttcggg acgccctcac gggagatccc ctgccgcgtt tcaccgccca tctcgtggat
    56581 acggggtggc gcgaggggga gcaggggagg atcacggccc ggcaggacgg ccggaaccgg
    56641 gacacccttg tgaaggccga tggatacttt gaaacgggat ggtttcttcc ccgggccttt
    56701 cctgggcgga gcgtggagcg cactgtggtc ctttggcctg tccaggggga gatggtcttc
    56761 tccgccgtgg acgccctcac gggagacccc ctgccgcgtt ttaccgccca tttggtggac
    56821 acggggtggc gggacgtgga aagatgcccg gtggcggtgc ggcaggacgg ccggaaccgg
    56881 aacaccctgg tgaaggccga tggatatttt gaaacgggat ggtttcttcc ccgggctttt
    56941 ccggggcgga gcgtggagcg caccgtgcct ctctggcccg tccagggaga aatggtcctc
    57001 tccgccgtgg acgccatcac gggagacccc ctgccgcact tcaccgccca tttggtggac
    57061 acggggtggc gcgaggggga ccaggggaaa ctccgggtcc ggcaggacgg ccagaaccgg
    57121 aacacccttg tgaaggccga tggatacttt gaaacggggt ggtttcttcc ccggaccttt
    57181 ccggggcgaa gcgtggagcg taccgtgccc ctctggcccg tcgtgggaga gtacggcgtc
    57241 cgggtgcggg acgtcctcac gggggagccc ctggcgaccg ttaccgccca tttggtggac
    57301 cggggatggg gggatgcgga aggagatgcc ctgacggtgt cgcaggacgg gaacaaccgg
    57361 agtaccctgg tgaaggcccc ggcctatctg gagagcgggt ggtatcttcc ctgtgcggtg
    57421 ccggggaaaa gggtgaccta tcccgtggcg ctctttccgg ccaatccccc cggtcgcatc
    57481 gaggtgcggg tggaaaactc ggccacgggg gccccgattt cgggggcttc ggtctctgcg
    57541 ggcgacgggc gcatttttcg gaccgatgtc tcgggaaggg cgttctggga ggggacgggg
    57601 cacttcgttc ccctgcgctt ttctgcctcg gctcccgggt accttggtgg agccatgccg
    57661 tcttttctct gcacgggaag gcagggaatc tccgtgaccc ttcccctgga tgaggtggtg
    57721 tcccccggaa ggggagggct cctggtggtg gcgcgcgatg gagaaaaacg tccccttcct
    57781 agcgcaaaaa tttttcttgg caagggagat cagcgaggat ggttgtccgc cgacgagacg
    57841 ggaagggcgg tctttcggga gatgggaacg gggatctaca cggtggcggt ggaggctccg
    57901 ggacatctct cttggagtgg gcgcgccgtg gtgcgtcctt cggcggtgac gtctcttgcc
    57961 cccgccttgg cgtcgctttc cgatacgcga cgcccggccc ccttcgctcc gcagctcgtt
    58021 tctcttcctg gaggggtaac gcttcgccgc agcgaaacca cgccgctggt ggtgacgcta
    58081 cgcaacaccg gggagatcgc cgggcgcacc gcttgtaccc tggaactgcc gtcggtgtac
    58141 cgggaaaccc gggatctggt cctcggcccc ggagagatga gggagctgga ctttcaggtg
    58201 atgccccccg tcgacgctcc gggggccacg tttccctata cggtttctct ggaaaaggga
    58261 aatgccctca cgggcatggc caccgtggag gcgccggggt gtgaggtctc cgtccgcacg
    58321 gacaaggggg cctaccggga aggagataaa ctggctctta cagcgcatat cgtggtggcg
    58381 ggcggtgccg cacgctccga agatcttcgg gaaacccttg tgttccgggt aaacttcaat
    58441 gatgacgtgt ggcggcggga agtggtcctg gaaaagggcg aaggcaccgt gacggtggag
    58501 gacatcccgg tggccttccg ggggaacaag cttctctacg gcgtctacga cctctccggc
    58561 accgcccggg ccctcaacgc tctctacgtg ctctccgatg gggagatgaa catgattccc
    58621 gatcgccccc aatacgcggc gggggaagat gcccacatcg ctctccgggg caccccgggg
    58681 aaagcgatct ctctggaaag cgcgctgtgg caggggatga aggaggtttc cctctcctcc
    58741 gagggcacgg gagaagtggt ggtgcctctg gcggcggaca tggccacggg gagctacgtg
    58801 atctccggcg atggggtggc cctacgcctg gacgtgcggg gcaaagagat caaaattctt
    58861 gatcgacgtc tggagcgcgc cggcgacgag atccgccttc tctggagcgc ccggagtcgg
    58921 ggggtgcatc gttgttcctg gtccgtgacg gaacttgacc gggaagggca ggaggggagc
    58981 gaagtggcct cgggggatgt ggagctcccc gccgacggaa gtctggcggt ggtttccttc
    59041 tccgagaagg gggtttgggg cgggataacg gaaggagaaa tcgaaagccc ggacagaact
    59101 tcggagagtc cccggaaaga gctggggggg tatcttctga ccctccggga tggggcattg
    59161 gtgcctcccc tcgcggtggt gcggtattgg gaatagggca cggcccgttc tttggtgcgg
    59221 atgtgccctg tggcgggagg tcgggggaga ggggggcgga ggggagctgg ggaaagagcg
    59281 gttcctcgtt gcgccccttc cgccgacagc gtagaatgcc cgaagaagag ccgttctgat
    59341 cggggcggga agaccacctc cggcggattt tcctccgggg gacaggaagc gccttctgga
    59401 ggcgctacgg gttttcggaa ggtccctacc gcccctttgc ggcattttag aaaaaaacgg
    59461 gtggcgccat ggagggccca agggagtgaa tttgatgtca agcgaaaagc gggaatcgcc
    59521 ggacgaagtg ttccgttcgc acgcggcccg acaccgggag ttggtggagc gcaccgaaga
    59581 acactgcgct tcggcgctcg ccgccgctgc ggctttgctg gaagaaacct tccgcagagg
    59641 aggcaaactc ctgatctgtg gcaatggggg cagcgcggcg gattgtcagc accttgccac
    59701 ggagttcatg tgccgtttgc gctcgtcatg tcaacggatt ccctttccag cggtggccct
    59761 caccacggac acgtcctttc tcacggcgtt tcccaacgat tattgttttg aggatatttt
    59821 tgcccgtcag gtggaggccc tttgcgaccc cggagacgtg cttttggcca tcagcaccag
    59881 cggcaaatct cccaacgtgc tccggggggc ggaagcggcc cggagcaagg gggggcgagt
    59941 ggtggccctt tgcggcgcgg gggggcccct ggcggagcag gcccatgtgg tgctggcggt
    60001 gcccgccacc gagaccatgc tggtgcagga aatgcacctc atgctggagc atgccctctg
    60061 cgtcgccgtg gaagatcgct ttctccggaa tccctggacg atctccggag cttgtcgggg
    60121 aaagtctccg gcggagggcg agggatcccg atgatcctgg tgacgggagg ggcggggttc
    60181 atcggcagta atctggtggc ggcgctgaac gaccggggac accgagatgt ggtggtggtg
    60241 gaccacctgg cccgggggga caagttccgg aacctgcggg ggctggcggt ggcggattac
    60301 cgcgatcggg aggacttcct ccgggagctc cgggggggat cctgggatcg cgtggcggtg
    60361 cgggccattt ttcaccaggg ggcctgcgcc gacacgttgg aatccgatgg tcaatacatg
    60421 atgcgaaata atttcgagta cagcaaaacc cttctccact tcgccctgga ccggcgcatc
    60481 ccctttatct atgcgtcttc ggcgtcggtc tacggcctgg gaaagcgggg tttttccgaa
    60541 gacccggaaa acgagtttcc cttgaacgtc tacgcctatt ctaagcacct tttcgaccta
    60601 tacgtccggc gcttgctccc cacggcgggg agtgcggtgg tgggtttgcg ctatttcaac
    60661 gtgtttggcc ctcaagaaat gcacaagggg cccatggcgt ccatggtgta ccaagcgttt
    60721 ctccaactgg aaaccacggg aacggtgcgg ctctttggag cggcggaggg atttgacgcc
    60781 ggagagcaac gccgggattt cgtggcggtg ggggatgtgg cccgtgtgaa tctctttttt
    60841 ctcgaccatc cggaagtctc ggggattttt aattgtggta cggggcaagc gcgttctttc
    60901 aacgacgtgg cccgacagct catcgccttc cgggggagcg gcaccatcac ctatgtgccc
    60961 tttcccccag aactgacggg gaagtaccaa aacaacaccc aggccgatct tggggccctg
    61021 cggcgagtgg gatacgaccg agcctttcct ccgcttgagg agtcgctgga ggcctatgtg
    61081 gggatcctca ggcgacagga aggttttttg acggcgccat gacggaaaga aagggcagtt
    61141 cggaagaaat gacccccttc tggaagggag aagcggcccg atttctccgg ggtcaagggt
    61201 cccgaggatc cttcctggtg gtgggggacg tcatgttgga tcggtacgta tcgggggacg
    61261 tggagcgcat ttcccccgag gccccggtgc cggtggtgcg ggtgaagggc cgccggagcg
    61321 tccccggggg ggcggggaat gtggcgctca acctcgttcg gctgggggcc accgtgacgc
    61381 ttttgggcgg cgtgggcgac gacggggcgg gggaagagct ccggtccctg tcggaacggg
    61441 agggaattcg cttttgcggc gttttagatc cctcgtcctg cacggtgacg aaaacccgaa
    61501 tcgtggcgtc gcatcagcag atgctccgtc tggacgagga ggtccatccc ccccttccag
    61561 aagagagcca ggaccgtctc ctggaggcgg tgcgcaccgc gctccgatct ccctttgcct
    61621 ttgacggggt gatcctttcg gactatggca agggggtgtg ttttcccgag ctgtgccagg
    61681 aggtcatagg ggcgggatct cgggcgggga ttcccgtgct ggtggacccc aagggaacgg
    61741 attgggagcg ctatgccggg gcgacgggca tcacccccaa cctgcgagag ctggccgccg
    61801 tggccccggg ggtcttcgat gggagagacg atgggggcgt cgtggcggcg gcccaggagg
    61861 tgcggcgtcg atacggagtt tcctggctcc tggtgacccg ttcggaggcg ggcatgaccc
    61921 tggtcctgcc ggagggaggg gtccacgagc gggcggcggc gcgggaggtc ttcgatgtgt
    61981 ccggagcggg ggacacggtg gtggccaccc tggtcttctt tcttgcggcc cccctccccc
    62041 ccgaagatgc catgcgctgc gcgaacctcg cggcgggtat cgtggtgggc aaggcgggca
    62101 ccagcccggt gacccgagac gagctgctcc gggagctcgg aagcggcgat ttccccggag
    62161 aagttcggga aaaaaaagtg gccccgaggg acgaggcgat ggcccgggtg gggtcgtggc
    62221 gacagagggg gctccgggtg gtggccacca atggttgctt cgacatcctc cacgtggggc
    62281 acctctcctg tctggaaagg gcccgggccc tgggagatcg gctggtggtg gcggtaaatg
    62341 acgatgcctc cgtccggcga ctgaagggcc cccgccgtcc cgtcaatccc caggaggccc
    62401 gggtggccct tctggcgggg ctttcctgcg tggacctggt ggtgcccttc gacgaagaca
    62461 ctcccgagga gctctaccgt cgcctacgcc ccgacgtgct ggtcaagggg ggggattacg
    62521 ccccggaaga cgtggtgggg cgagggtacg cgggggaaac ggtgatcttg cctctggtgg
    62581 aggggtactc caccaccgca cttttgagaa agagcgcgga ggggggggga tcgtgaccgg
    62641 cgggaggagg tttgtgctcc tggaccgcga cggcaccatc atggaggatc ggcactacgt
    62701 gggggacccc cgggacgtgg ccctgctccc ccgggcggga gaggggcttc gccttttgag
    62761 gcgggcggga tggggtctcg tggtggtgtc aaaccaatcc ggagtgggta gggggttttt
    62821 ctccctggag gccgtggcgg aggttcatca gcgcctgaca gagctcctgg cggaagaggg
    62881 cgtgacgatc gatgcctttt acgtgtgtcc ccaccttccc gaagaggggt gctcctgccg
    62941 aaagccccgc ccaggcatgg gactccgggc catggaagac ttccgatttt ccccccgcca
    63001 ggggctggtg gtgggggata aagaaagcga cgtgctcttc ggtaagaacc tgggcatgag
    63061 ggcagtgctc ctctcgggag acgcccggaa gggctctggg gcggatgcgg tgcttcccca
    63121 cctggagtcc gtggcccgat gggcggagac ctggcccgag gagggcgatg aggcatgatg
    63181 acggaaatcc tcccggggga gcgcgtgctc ttcgtccggt acagcgccct gggggacgtg
    63241 atccaggcat cgcccctggc ccgggccttg aaggcccaat ttccaggggt ggagttgacg
    63301 tggttggtga cttcccccct ggacgacctc gtgcgaggtc agccctttgt ggacgatgtg
    63361 ctggtatggc accgatctcg gggagagcgg ggcttcgtcg aaatgatccg agaggtgcgt
    63421 cggaggaagt tccaccggat ggtgagcgtc cagagctccg atcggtccgc ccttttggcc
    63481 cttctgtcag gcatcccccg gaggtgtggc gctcaccgat ggttttccgg ggtctatgac
    63541 gagggaattg atgacttgcg aaaatttttg ggattccctc cgttgcgggg ggcgctggag
    63601 gtgacggaag aggggcgggt ctgggcgctt caagagctag cctccttgcc tcctcggagg
    63661 gtggggtttg ccataggtgc ttccaaggcg gtgaagctct ggcccgacga gaggtggcag
    63721 gagttggccc gggagctcgt ggcccggggg gtgggagtgc tccttctggg cgatggagaa
    63781 gaagagcgcc gccgggggga ggccctggtc cgggatctgc ctttcgcgca aaacgccgtg
    63841 tctttctact ccctgcggca aacgctggcg gcggtggggg gggtggacgc cctggtgggg
    63901 ggagattcgg gggtgctcca catggcagtg gccctggggg tgcccacggt ggggctcttt
    63961 ggccccacgc tcccggcgca ggtgtctctg ggcggcgtgg attgtcccct cctggcggac
    64021 tgctcccagc ggggttgcca gcggtgggag tgccccgagc cttgcctgaa aactattggc
    64081 acggcggcgg tccttggggc gctggaagaa ttgggagtgg cttagtccca cctcttggcc
    64141 ctgccctgga gatttctcct ggaagatgaa gaaaccttga aatgaccccg aggcttttcc
    64201 cttttcgata tctcggggtc gtgttatgct gaagccacat gaggaggctt gaccgatgga
    64261 cgacgctctt ccctttgcgc tgccggatat gggggaggaa gaaacgcagg aagtggtggc
    64321 atgcctccgc tccggatggc tcaccacggg accccgaacg aaacgtttcg aggaagacat
    64381 ggtggcggcc ctggggggag aagtgcacgc cctggcggtg tgctctgcca cggcggggct
    64441 ccacttggcg ttggaagccc tgggggtggg gccgggggac gaggtgttgg ttcccaccta
    64501 taccttcacc gccacggcgg aggtggtgcg ctacgtgggg gcccacccgg tcttcgtgga
    64561 ttgcgacgaa tccacgctga acatggatcc caccgaggcc gaagcgaagg tcaccccccg
    64621 gaccaaggct ctcttgccgg tacacgtggg agggctccct tgcgatatgg gccggatctt
    64681 tgacctggcg tcccgtcggg ggttgcgagt ggtggaagac gcggcccacg cctacacctc
    64741 tctgtggcac gggtgtcccg tcggcgccct ggatagcgac gcttcggtgc tcagctttta
    64801 cgccaccaaa accatcgcca cgggagaagg gggcatgctc ctcacccgga acgatgacat
    64861 cgcccgtcgg gcccgggtca tgcgtctcca cggcatcagc cgagacgcct ttgaccgcta
    64921 tacctccccc caagcccctt ggttttacga ggtggtggcc ccggggttca agtacaacat
    64981 gatggatctg gtggcggcca ttggcatcca ccagttgcgc aaggccccgg cttttcggga
    65041 gcgacgggaa cagatcgccc tgcggtacga cgacatgttc cgggatcttc cgctaaccct
    65101 tcccgcccac gtccgggacg gtggggaccg tcattcctgg cacctctaca tcgtgcgcct
    65161 gacttcggac gccccacgat cccgggacga gtttatccag ggcctccagg gacggggcat
    65221 aggcaccagc gtgcactata tcccgttaca cctccacccc tattggcgag acacctatgc
    65281 cctgcgatcc ggggactttc cccgggccca gagggcctac gagggcgccg tgagcctgcc
    65341 catctactct cggatgacgg atcaagacgt ggagcgcgtg gggcgggcgg tgcgcgagct
    65401 tctgtcgtga cggggagctc catgcctcct cttcgccggg tgctctttat atgtcgttac
    65461 ttcccccccc gatggaacat aggcgcggtg cgaagctggg gattgacccg atacctgccc
    65521 cagttcggat gggaaacggt gctctttgtg ccccctttgg aagaagggcg ttggcccaca
    65581 gggattcgca tcgtcgaggc ggattggctg tctctgggat accgggtgaa gcgtctggtg
    65641 ggattccccc tgccctggcc ccgtcgagat ctggaagacc tggtgcgtcg ggagggcatt
    65701 gaagccatcc tttccacgtc gacgcccatg atggcccatc gggtgggggc cgcgcttaaa
    65761 aaaagcctgg ggatcccctg gatggcggac ctccgggatc tatggtctca aaaccccttt
    65821 tcccgccgag gggccttcgg aaggtggcgg gagcggcggg aagagctccg catcctggcg
    65881 ggggctgatg ccctggacac ggtgacggct ccgggggcgt cgcagctcgc cgcgctgcac
    65941 cggcgtcccg tgtggcacat cgccaacggc tttgaccccg agaccgttcg tcccgaaggg
    66001 gcccccgggg acggtcggtt caccctatgt tacacgggga gtctttacgc cggacgtcag
    66061 tcctttttgc ccttactcaa ggccctggcg atcctgcggc aagacgaccg attgtcgccg
    66121 ggggatctgg cgttgcgcat tttttctccc ccctccgaag aactgcgccg ccaaatatct
    66181 tcgatcggcg tggaggactt cgtgatccag gaagggatgg tatcccgaga tagggcgctg
    66241 gaggcccagc gccaggcgtc ggtgctcgtc ctcctcaatt gggaagggag cgacgccgag
    66301 ggcatcatgt ccggaaagat cttcgagtat ctggcggccc ggcggcccat cctggcggtg
    66361 gggggaaacc ggacggtggc cacagatctg gtggcccgca ccgaaacggg gtaccatgag
    66421 aacgaccccg gagccctggc ggagctttta cggcgcctcc ggagatccca ttgttctggg
    66481 gggatcccct tttctccccg gaagagcgat gtggaggcct gctctcaggt ggagatggcc
    66541 cgcaaggtgg gggcggtgct cgatgccctc accgggaaag gagacgcgtc atgggtgcct
    66601 cgggcgatac cttgaagatc gtcctcatcc ttccggagct cgaagaagga ggagtggaaa
    66661 gacacgtggt gtccctatcg gcggcctatg tggcccgggg acacgaggtg tgggtggttt
    66721 ctcggggggg agccctggtg agggccttgg caccaggggt caggcatggg acaatgccgg
    66781 tgcacgccaa aaaccccttg acggcggcgt ggagtgccat agccctggcg aaggagattc
    66841 ggagagagaa gtggcacatc atccacgccg agtcccgggt gcccgcctgg gtggcgtggt
    66901 gggcctccgc cttggcgggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    66961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna
    67021 cgccgagtcc cgggtgcccg cctgggtggc gtggtgggcc tccgccttgg cgggagtccc
    67081 ctggggggtc acggcccagt cctgttactc ctgcaatgcg ggtcttttcc cctttcgccg
    67141 ggcggatttt gttgtggcct gtagtgaggc ggtgcgtcgc caccaggagg cgtgccaacc
    67201 cggcctttcc atggaagtga ttcccgatgg cgtgccctcc ccaggccttt cctggcgggg
    67261 cccctcgtcg ggcgaccccg ttacctttct ttttgtcgga aggctcaccg ccgtcaaagg
    67321 cattgatctg gcggtggacg ccttcctgga acttcccgaa gggctacctc cgtggagatt
    67381 tttggtggtg ggggatggcc ctctgaggga gaatctggaa aataaggtgc gaagagcggg
    67441 gcaggagggt agaatttcgt ttttgggctt ccagcaggac gtctttcccc tcatggcacg
    67501 atcctcgtgt ctgatctttc cttcccgagt ggaggggatg cctctggtgc tgtcggaggc
    67561 catggccatg ggggtccctg tactggcctc ggacatcgaa gcggtccgag gcatggtaaa
    67621 agaatcccat gcccttgtgt ccggggaggg ggcgggactt tgggcgaaga agttggagga
    67681 aataatcata aaaggagctc cgcccccctt gacgccctcg ggagattttg gttttgatag
    67741 catggtggag cgatacctcg cgacctaccg gagggtcatc gaagaaaagg caggtgatgt
    67801 ctccttaggg ggtaaagccc ccgggtccga gggacgtccg gggcgcgaag ggacgaacga
    67861 gagaggggag gggcgcggag acctatgctg aaggttttta tcgtgtccga ttttaccggg
    67921 gaaacggcgg agcacgtggc ccgggcggcg gcgagtcagt tcggcgccga ctccacggcc
    67981 tttactcgcc accgctatgt caacaccaaa gaacgagtgg aggaggtggt tggtcacgcg
    68041 tgggattccc aagcggtcat cgtctgcacc ttggtggatc atgcgttgcg caaaatgatc
    68101 ggcgaactat cccaggagcg aggagtgccc ctggtggaca tcctggggcc ccttcttcga
    68161 gccttcgagg acaggctcgg gcgtactccc ttcgaaacgc cgggattgct gcgacgtatg
    68221 gacgaagagt atttcaagcg ggtcaaggcc atagagtttg ccattcggtg cgacgacggc
    68281 cggggagccg agatgctccc ctcggcggag gtggtggtca tcggcatatc ccgcaccagc
    68341 aagacgcccc tttccatgta tctggcccat cgcggttttt tggcggccaa cgtgcctctg
    68401 gtgcccgaag ccactcctcc ggaagagctt tttcaggtga acccggcgaa ggtggtgggg
    68461 ctgtacatca tgccagaaaa gctcgttcag atccgtcggg agcgactgcg catcctggga
    68521 ttggacgccg atggatccag ctatgcccgg tgggatcggg tggacgaaga actccgattc
    68581 gcccgcgacg tctttcgccg ggtggggtgt cgcacctacg acgtgactaa tcgggccatc
    68641 gaagaaaccg cccaggaagt gctggatttt ctccgggcgt aggggggtat tctgttttac
    68701 cctgcgggat gcccctcccc gagatttgaa ttggtagtca tttaagaaat cacaaaggag
    68761 gagacgagat tatgggagaa caatttgtct atgatttttc ccagggagac gcttccatga
    68821 agaaacttct gggaggtaag ggggccaatc tggcgcagat ggtacgcctg gggcttccgg
    68881 ttcctccggg gtttatcatc accaccgaag cttgcaaggc ctactggaag gagtctgagg
    68941 gccttttgga tcgaatttgg cccgatgtga aggccgccgt ggggcgtctg gaagccacca
    69001 cgggaaaagt tttcggaggg gatgaccctc tgctggtgtc cgtccgctcc ggggctccgg
    69061 tgtccatgcc agggatgatg gagaccgtac tgaacctggg actgaacgac aatactgtgg
    69121 tgcgtctggg agaggcggcc aagaacgagc gcttcgccta cgacagctac cgccgattca
    69181 tccagatgtt ttccaacgtg gtgctaaaca tagacagcgg agagttcgaa aaccccttga
    69241 ctcaactcaa gcgtcgtctg gggctatcct acgattacca gattccggcg gaggaatgga
    69301 agaccctgat cggcactttc aaggaaatag tccttcgctc cacggggaaa cccttcccca
    69361 tggacccctg ggatcagctc cggagcgcca ttgaggcggt ctttgccagt tggaatattc
    69421 cccgggcggt gacctaccgc aagatccaca agatctccga tgacctgggt acggcggtga
    69481 acgtggtggc catggtcttc ggcaatctgg gagaagactg cggcacgggg gtgtgcttca
    69541 cccgcaaccc ctcccacggg gagaaacatc tctacggcga gttcctcatc aacgcccagg
    69601 gagaggatgt ggtggcgggc attcggacgc cggagcccat tgcggacctg gaaaatgtca
    69661 tgccggagat ctaccagcag cttttggata tttcaaaaaa ccttgaaaac cattacagag
    69721 acatgcaaga tatcgagttc accgtggagc gaggaaagct ctacatgctc cagacccgga
    69781 gcggtaagcg cacggcccgg gcggcggtga aagtggcggt ggacatgcta aaagaggggc
    69841 tcatcgatgg aaagacggcg gtctctcggg tgactccgga acaggcggag cagctcctcc
    69901 accggcaggt agaccccgat gcctccgttt ccgtcctcgc caaggggttg cccgcctctc
    69961 cgggggctgc ggcgggagtg gtcgttttcg accccgacga agcagaacgt cggggggagg
    70021 cgggagataa ggtgatcttg gtgcgtccgg agaccacgcc ggacgacatc catggcctct
    70081 ttgccgccca gggagtactc accagccacg gggggatgac gagccacgcc gccgtggtgg
    70141 cccggggcat gggtaaaccc tgcgtgtccg gatgcgaggc ggtggagatc cacctagatc
    70201 agaattattt ccgggtgggg gacgtggtgg taaaggaagg ggactttatt accctggacg
    70261 gttcccgagg acatgtgatc ctgggagagg tccccatgat ccagccttct tttgacgatg
    70321 acttccggcg ccttttggaa gaggcagacc agttctcggt gatggaggtc tgggccaacg
    70381 cagacactcc cgaagactcc gcccgggcgc gctccttcgg ggcccaggga gtgggacttt
    70441 gccgcacgga acacatgttc atggcttcgg atcgcctgcc ggtgatgcag cgcatgatcg
    70501 tggcggagga tcgagatgcc aggattcggg agctcgacct cctccaggta atgcaaaccg
    70561 aggacttccg gggaatcctc gccgccatgg aaggtttccc ggtgaccgtg cgcctgttgg
    70621 atccccctct acacgaattc cttcccaagg aaggcgaatt gagggatgaa atcgccgccc
    70681 ttcctgaaag cgataaaaag gagcgtgcgg ccctggagcg cgtcctggcc aaggtattgt
    70741 cgttgcggga gtcgaatccc atgctgggct tccggggatg ccgtctgggc atcgtcttcc
    70801 cggaaatcta cgaaatgcag gtccgggcca tcttcaccgc cgcctgtgat ctcaccgccg
    70861 cagggaagaa agtcctcccc gacgtcatgc ttcccctgat aggcacccgg gaagagatga
    70921 gacgcctcaa ggacatggtg gacaccgtgg cccgggagat catggagagc cataatgtgg
    70981 acgtgcccta tcacgtgggg accatggtcg aggtaccccg ggccgccatg gtggcggatc
    71041 agttggcgga gttcgccgaa tttttcagtt tcggcaccaa cgatttgacc cagacggcct
    71101 tcgggtactc ccgggatgat gcggagggga aattcctggc ggactacgtg cgtctgggca
    71161 tttttgacga aaaccccttc catgttcttg accgcgaggg cgtgggacgg ttgatgcgca
    71221 tcgccgtgga agacggccgt cgggcgcggc cggatatcaa gataggcatc tgcggagagc
    71281 acggtggcaa ccccccgagc atcttcttct gccacgagct ggggctcaag tacgtgagct
    71341 gctccccctt ccgggtgccg gtggcccgcc tggcgaccgc ccacgcggcc ctggcatcgg
    71401 cgcagtagat ccggcgcccc ggaggcttaa gatagcaaag ggggcccgcc atggcgggcc
    71461 cccttttttc atctttttca ttctgagaga gcgtcggcgc ccccgtaggg cgggttgtca
    71521 gttggacagg ccgtactgtt tgagtagatc ctggtatcgc tcctcaatct ggttcgccgg
    71581 gctggggtct ttttcgtata tgttctgggt ggtggtgggg gggacgaaaa tgggatccag
    71641 ggggcgcacc tcggaagcac ggggaagggc ggcggtggcg gtccccgaag cccctccgag
    71701 ggcgtactgg gccaggagag gttcgtcggc ccccgccagg tagagccgag gggcttggag
    71761 gtcattttcc gcctcgaagg gatcgccgcc atgctgggga cacaccgccg taggcacgag
    71821 gtcgtaggga agaagcactg ggagggaaga gcaccccttg gaggccagat accctgtgat
    71881 tttgcacacc ctcacctcct gcacctgggc gctttctttg tcggaaaacg tcggaggaag
    71941 gcccagaagg gccgccgctt ttgtgacgaa ggcgttccat acgggggcgg caatgactcc
    72001 ccccgtattg gtttttccct tgcctatggg agagtggtcg tcgtttcctg cgtagaccac
    72061 caccaccagg tttggcactc ctcccacaaa ccacgcgtcg cggaagtcgt tggccgtccc
    72121 ggtcttaccg aaagtttccc aacccttgac ccgggctcgg cttcccgtgc ccgccaggac
    72181 cgcgtcctga agcatggaac gcaccgtagc cgccacctcc ggagccaagg cggggacaag
    72241 ggaagcgccg ttttgctcca gcacctctcc agtgttgctt cggacctctc ggacgaggaa
    72301 gggtgagacc cgaaatccgc cattggcaaa gggggcgtag gccgtcacta gatccaaggg
    72361 ggtgacgctg gcggacccca acgccaggga aaggtcccca gtgatgtggg gggtcgtaag
    72421 ccccatgcgg cgcaccacgt ccaggatttt gtcgggtccc acaagctctg ccaagcgcac
    72481 ggcggcggta ttgtaggaat tgatgatggc gttaagaagg gtcatctcgc cgtggaactg
    72541 cccgtcgaag ttctggggac tccatccgtt gccgtaggag aggggggcgt cgaggattcg
    72601 gtccacgggg cggatcccct gttccgcagc ggcggtatat acgaagggtt tgaaggccga
    72661 tccgggctgc cggaaggcct gggtggcccg gttgaatttc gatacgttga aatttttccc
    72721 ccccaccatg gccaaaacct ctcccgtttt tgggtccaac gccacgatgg ccccttcagt
    72781 tttcagtccc tgaatggctt cttcggcggc gtgctggagc tccaggtcta gcgtggtgtg
    72841 caccgtgagc ccctgttggt agacctggtc cgtgccgtac ttgggcaaca ggtgttnnnn
    72901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    72961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaccg tgagcccctg ttggtagacc
    73021 tggtccgtgc cgtacttggg caacaggtgt ttgaaaagta cttgggacac gaagtaagga
    73081 gcgctactgg attccagccc cgatttggag agcttggtga gtttgagggg ggtctctttg
    73141 acctctgtgg cttcagaagg ggagatgaat cccacatcca ccatccgtcg aagcacgtag
    73201 ctctgccggc tcaccgccag ctctggcgac cggcgagggc tgtacttttc cggcgccgcc
    73261 acgatgcccg ccagcatgga agactccccc aggttgagct gctcggcgct tttgtcatag
    73321 taggtccggg cggccgcccc gatgccccag gccccatggc caaagtatat ggtgttcagg
    73381 tacatctcca ggatttgatc tttggtgaaa atcttttcta tccggaacga gagcagagct
    73441 tccttggcct tgcgctccat ggtggtttcc cgactaagga agaggtttcg ggccaactgc
    73501 tgggtgatgg tgctggcccc gtgcccccga tctcccgtca ggatggagcg taggatggca
    73561 aaggggcgga cccctccgtg ctcatagaaa gacgagtcct ccgccgccag gatggctttg
    73621 accatccagg gggagatttt ttcgaagggt acccagacac gatcctcttg aaagagtcgc
    73681 gccagaagct tgccgtggcg atcaaagacg tgggtggcga ggttgggctt tcgctggatg
    73741 atctcgtcgg tggaaggcag ggtggcgctg agcttttgga catagagagc cccccaaacc
    73801 gaggcccccg caagggccac ggctcccacg aggagcaaga tgacgaaaat cttcacaagg
    73861 caccctgcac atcctctttt ctgagggggg ggtgtttttt tctttccgga aggatcttgt
    73921 gggctgggat cgggcgtccg acggttcgtc atggagtcat acctccaact gggagtctcc
    73981 ctaggggggg cgatgtcgcg atcctcgggg aggaggaccc cttccatcgg aggacacgcg
    74041 ctacgacaaa ttatagcata aactgcgggg tcgcgatccg acggaagatc cgacggaaga
    74101 agggatcgca ggcctgcaga agagccgaca ccccgtatcc cttttgcccg cagagtcccc
    74161 gtggcgcgca tggaaatagg tcatgggggg gatcaaaaga ccccagccct ttttcacctc
    74221 aagagtgggg gcccaagagg ggcaatttgc tcctcagggg gcttgcgcga tttttgagag
    74281 cgtggtatag tgtcccctgt cgccgctcga agggcggcga gcaagcacct tgccaacaga
    74341 atacagcgca caagaagttt tcaagccagt gtttttgtaa gctatttatg gagagtttga
    74401 tcctggctca ggacgaacgc tggcggcgtg cctaacaca
//
LOCUS       DAOOER010000004        65977 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_450_length_65977_cov_9.265450, whole genome shotgun sequence.
ACCESSION   DAOOER010000004 DAOOER010000000
VERSION     DAOOER010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 65977)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 65977)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65977
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_450_length_65977_cov_9.265450"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="PL162_00935"
     CDS             complement(<1..474)
                     /locus_tag="PL162_00935"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HQF90624.1"
                     /translation="MRLRLEASLREPDGRWRLAAEEGHHLMVRRCASGDRVEGLLPGY
                     RVELRLERRGDVWYGWEESRRSDRGSPVRLTLLAGVLKEDAWDALLRMATEVGVSRVI
                     PLLCVRSVPRLGGERLSARRERWRRIVGEATKQCGAPEPPEIAPPRSVEELEEGAL"
     gene            complement(437..1327)
                     /locus_tag="PL162_00940"
     CDS             complement(437..1327)
                     /locus_tag="PL162_00940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HQF90625.1"
                     /translation="MSVEESFWWYVTLEGRDGDGEVLESLVDLSGSIGAEHQEGTDRC
                     RVRAFYRSSQDVAFWLKRVNEVLEPWPRISVVDVGKVENQRWHVAWQESFPPLEVGKT
                     LVVMAPWHRGAEPPGRRPLYIYPGSAFGTGYHETTQIMLELMESTPLEGKAVADIGTG
                     SGILAIAAARLGARSVVARDLDPAVMPEIQENCRLNAVPPEVVTAEVGDLLKDFRHRV
                     DLVVANILLEPLLTMLPHLREALLPGGRAIFSGMVQKERERFLEALREHRFDVQEERS
                     RGEWWGVAVAPEAGSEPSGA"
     gene            complement(1568..2722)
                     /gene="dnaJ"
                     /locus_tag="PL162_00945"
     CDS             complement(1568..2722)
                     /gene="dnaJ"
                     /locus_tag="PL162_00945"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HQF90626.1"
                     /translation="MPGTHKDYYQILDVPRDASAEDIKKAYRKLVRKYHPDANPGNRE
                     VESRFKEISEAYEVLSDPQKRTQYDQFGYVGDMPGGGAGPFGGGEPFGDIFGDIFDSF
                     FGGGGAGRGRTRNVNAPRRGADLEMEISCTLEEVFSGVPKEVQIPRWETCKRCSGQGA
                     EPGTSAETCSACGGRGQVEHAQRTPFGQFVSVVPCTRCGGKGKTIAAPCRSCGGDGRT
                     RHRHRVEVRIPPGADTGTRLRMVGEGESGVNGGPSGDLYLVVTVATHPRFLREGDTLH
                     VQIPVAFPQAALGSSAEIVMLDGVVERVTIPAGSQPGDLVRLRGKGMPRLRSSGRGDL
                     LAHIKVEVPRELTERQRGLLVALAQEMDVPVEENEGLLEKIKGWFGRSEG"
     gene            complement(2818..4647)
                     /gene="dnaK"
                     /locus_tag="PL162_00950"
     CDS             complement(2818..4647)
                     /gene="dnaK"
                     /locus_tag="PL162_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HQF90627.1"
                     /translation="MAKVVGIDLGTTNSCVAVREGDGITVIANAEGSRTTPSVVATTK
                     EGERLVGQLAKRQSIVNPDNTIMSIKRRMGSDAKVTMGGKGFTPQEISAMVLQKLKRD
                     AEEYLGEPVTQAVITVPAYFTDAQRQATKDAGVIAGLEVLRIINEPTAAAFAYGADKE
                     GEHKILVFDLGGGTFDVSLLDVGEGVFQVLATSGDNHLGGDDWDTRVAEWMVKEFKSR
                     EGIDLSRDRMAMQRVREAAEKAKVELSSMPETTISLPFITADQNGPKHLELSISRSKF
                     EEMTADLLERVVGPTKRVLEDSGLAASAVDKVLLVGGSTRMPMVQKRLRDLLGKEPTK
                     GVNPDECVAMGAAIQGSILAGERTDIVLVDVTPLSLGLETLGGVFTKIIDRNTAIPTS
                     KSQVFTTAADSQPSVTVMVFQGERPMAADNVKLGEFNLDGIPPAPRGVPQIEVTFNID
                     VNGILNVSAKDKGTGKSQHITIQSSRLTEEEIERMKREAEMNEGADRQRKEAAEARNE
                     ADSLAYNAEKTLKDLGDKVEAPERARVEQEIADLREAASKDDLARTRQAIETLTKSLH
                     ALSQKLYAAQDQSGGEAPHPEGPKESPSGEGETVDAEFSDKEA"
     gene            complement(4745..5305)
                     /locus_tag="PL162_00955"
     CDS             complement(4745..5305)
                     /locus_tag="PL162_00955"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HQF90628.1"
                     /translation="MVKDMEEGPVNEGALGEVEDMERGGDLAVLREQLAQLEDERNAL
                     REEVARARADFYNFRQRSEREGLRQRELAAERAVTLLLPVLDNLDRAMESSGGGEGLR
                     QGVAMVRRLFFEALQELGVSLVEAEGAFDPARHDAVGTVPVKDPAQDHQVIDVVRPGY
                     ALGGRVLRPAMVRVGAHTPASDGGSR"
     gene            complement(5328..6368)
                     /gene="hrcA"
                     /locus_tag="PL162_00960"
     CDS             complement(5328..6368)
                     /gene="hrcA"
                     /locus_tag="PL162_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HQF90629.1"
                     /translation="MLSERQLEVVLAVVYEYIQGGDPIGSRVLSKRHLTGRSAATIRN
                     EMADLADMGFLEQPHASAGRVPTARAYRLYVDSILHRHRELPPEAEGWISEVRSQRRG
                     LEDVLSHISHLLSRVTSYMGVATLAPLDEVGLLRLDFVRLESTRVLAVVVLDGGLVHH
                     RVLTLPCEMSQDELDLLASRIHAVAVGRPWKDVRRDLIQYVVAELRDREMACRVALRE
                     LEGILSSQPTRIFMGGARRLLNLPDFHDIGRLQAIFSLLEEERSMAHLVERCAQEQGV
                     SVTIGEENPVEALKDCSLVLAPVSSAGQRAVVGLIGPVRMNYEGAIAVVEAMLAGLQD
                     MPEKAGAAQEVP"
     gene            6609..7256
                     /locus_tag="PL162_00965"
     CDS             6609..7256
                     /locus_tag="PL162_00965"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQF90630.1"
                     /translation="MGEPKRTEIILVRHGECAENREGVFRGRSDGPLTERGVRQAELL
                     AEEVARFFSPSAIFSSPLQRARNTARAIGERLGIPVEERSGFTNMALGPWEGQSKDLI
                     RETYPEEWALWIHNPERLALKGAETLDDVQRRAYANLDHLVRLHEGKAFVVVSHRAVL
                     KPLLAACLAIPSPYFWRLHLDTASYSVLWHERTRGYTCALLNQTRHLPDLVSEWT"
     gene            7271..8791
                     /locus_tag="PL162_00970"
     CDS             7271..8791
                     /locus_tag="PL162_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQF90631.1"
                     /translation="MATPALEIRHLSKRFGSFVANDDISLEVLPGEIHAVVGENGAGK
                     STLMKCIYGMHRPSAGSLALRGQEVVVDSPRTANHLGIGMVHQHFMLVPSFPVYRNVV
                     LGAEPGQWGYFDEGAALRRVEGLTKEFNLAVDPRSTTATLPVGLQQRVEILKLLHRGA
                     DILIFDEPTAVLSPQEIEGLFAILENFRRSGKTILFIAHKLREVLAVADRISVLRQGK
                     LEGTVRRDEVNEATLARMMVGRDLPALAPGAPPTSGKELLRAEGLWALGPRGEWALRD
                     VSFSVAAGEVLGIAGVMGNGQSELEECLCGLRHLREGKIFLGDRDITEATMASRREGG
                     LAYVPEDRLRTGIAPLARLWENMLLGHQREERFLRGGPLGFLRPGAIRPWVRRTMELF
                     SVKAASDMVQGGTLSGGNMQKLVMAREMAFDSPLLLVSQPTRGVDVGGIAFIHEQILR
                     HRRQGGGALLISSDLDEVLALADRVAVMYQGRIAALLPRKEASRELVGRYMLSGMA"
     assembly_gap    9232..9331
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9332..11113)
                     /gene="rlmN"
                     /locus_tag="PL162_00975"
     CDS             complement(<9332..11113)
                     /gene="rlmN"
                     /locus_tag="PL162_00975"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HQF90632.1"
                     /translation="MARVSGLELTLENWRDLLISLEEPPYRAEQIGDWIYRKKIFSFK
                     DMTNLSQGLRQKLTDAVDLILPDLVKERTSLRDGTKKFLWRLGDGATVESVFLPHATH
                     KTACLSSQVGCPLGCAFCATGKGGFVRDMTAGEITGHFLAMERHLGAPVQNVVLMGMG
                     EPLLNTAEVFKALRILNAPGMRHLGIRHMALSTAGVVPGIDALADSGLGVRLAVSLHA
                     AEDELRSQLMPVNRVYPLGELRQALLRYQAKTGDRITLEYALLEDVNDTLPHVEALGA
                     FVRGINAFVNLIPYNGDDGNFRCPPQDRMERFRRLLQERQVNVELRVPRGGDVEGACG
                     RLKSASEEGEDRPSRSAGDRPFSDRPRPPHREGEGPFRRERDDRPQAPRPDRFRGEPR
                     PRPEGSSGEDRPRRSAGDRPFSDRPRPPHREGEGPFRRERDDRPQAPRPDRFRGEPRP
                     RPEGSSGEDRPRRSAGDRPFSDRPRPPHREGEGPFRRERDDRPQAPRPDRFRGEPRPR
                     PEGSSGEDRPRRSAGDRPFSDRPRPPHREGEGPFRRERDDRPQAPRPDRFRGEPRPRP
                     EGSSGEDRPRRSAGDRPFSDRPRPPHRE"
     gene            complement(11261..12541)
                     /gene="glyA"
                     /locus_tag="PL162_00980"
     CDS             complement(11261..12541)
                     /gene="glyA"
                     /locus_tag="PL162_00980"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HQF90633.1"
                     /translation="MRQIMEMGMADLDPEIYGLTKSELSRQRDGVELIASENFVPRAV
                     MEAQGSILTNKYAEGYPYKKYYGGCEYVEAIEEIAINRAKELFGADHANVQPHCGSGA
                     NMAVYFAVMKPGDTLLAMSLDQGGHLTHGHPLNFTGKMYHVVGYGVTRETETIDYDAM
                     ERLAREHRPQVIVAGGSAYPRDIDFGRFARVAREVGAVFLVDMAHIAGLVAGGAHPSP
                     VPHADFVTTTTHKTLRGARGALALCTAQYAKDLDRTVFPGIQGGPLVQAMAGKAVCFR
                     LAMEPEFKVYAAQVVANAKALSRELVDRGYRIVSGGTDNHLILLDCKTSRGVTGKDAE
                     RILNEAGVTVNKNMIPFDQEKPMITSGIRIGTAAVTTRGMKESEMGRIADAVDRVLSR
                     PEDAAEHARVKAAVKELSGAFPLYPAFSEPWPTI"
     gene            complement(12904..15066)
                     /locus_tag="PL162_00985"
     CDS             complement(12904..15066)
                     /locus_tag="PL162_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQF90634.1"
                     /translation="MLKNLKIGVRLMLGFGIVLIIVVCMGGLGVYQTGLIRGEIGVLA
                     SRAMPQVELSSTLERYLLQALRCFQTYNLTMKQGDFNGVIQILGELERVLGEARELAD
                     REKMAEFLKSLDDLGGKVAEYQAQAKEAQKTNKAIGEAREKQDKAFLSYRTICSEFLA
                     LENKELDKEISDREDPSFVKDRLSKIEIVSRILSTGEEANVATLKAQLARDPAVIEKA
                     LGGFAPVNDYVDELIMISIRPKDKERMEAVRQATADYVAAAKVVVEGYTKLAALSQSL
                     ETASKGVLAVTNALSLEGFTQGKGVVQRASEALSLSSRVLSYGMVIAALLGALVALGI
                     VRSITKPLRFVVEHCRAIGDGDFTVSVDYQSGDEVGQLAHAFDTMVKELREVILRVKT
                     AARELAAASEETAASVQEVTATTMDLSHKNTELSGATTMGFAAVQETSEVLLELSSLL
                     QISANLARSATEQAERTLATASSGRATVDTAVHHMETIREKELETEKVIGGLSAFSAR
                     IGIISDTITGLANQTNLLALNAAIEAARAGEAGKGFAVVAEEVRKLAEQSQRGAREVA
                     ELVTKIAEGTQTAVLSVQESRARIEEGVGAVRQSGEALANIVDAAQHSVDDIGKIQKT
                     TSEEVSQSDRIVELIQRTAVVIEQAERHAQEFLVATGNVVSVMENVSAASEESSSMAN
                     ELDSLVNRFRVEEGDDPFALPMPAGATELAPLEEEEGE"
     gene            complement(15306..16328)
                     /locus_tag="PL162_00990"
     CDS             complement(15306..16328)
                     /locus_tag="PL162_00990"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HQF90635.1"
                     /translation="MTNPGTGEIPFVMDAHLDLAYDVELRRRRGERRVVAERYLPAFR
                     RGGVGAVISSLFIPNVFLPEMALRKALEQISALGADLKECDGAVVLCRTMEDLRAARR
                     RGAVGVFISFEGAEPLQGNMALLRIFFDLGVRGVGLVWSRRNEVGDGCHFFPENEGRP
                     GGLTPFGVDLVAAVEEMGMFVDVSHLNDAGFDDVARFSRKPFIASHSNCRALVPVARN
                     LTDDQLRTLGARGGLTGMNACDAFVSSAPREESRDLVDVLGDHVDHVKKVAGIAAVGV
                     GFDFCDQIRDALSDGGSIQTRDVMEGHGDLGRFTQALRRRGYDEGEINAVLGGNLWRF
                     LEKVLG"
     gene            complement(16339..17499)
                     /locus_tag="PL162_00995"
     CDS             complement(16339..17499)
                     /locus_tag="PL162_00995"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQF90636.1"
                     /translation="MRERGNLLIRGATVWTVGPRGILDRTDVLIKDGIIASLGPDLRG
                     GDGEELDGRGMVVTPGLIDVHTHVSLWEEGAPEAEGSGNEATNPVTPHLRVVDAINPR
                     DRAFAEARSGGVTMVQILPGSANVVGGQGAVVRTWGDTVEDMVRQAPSGMKAAFGENP
                     KNVYKGKNLWPSTRMGVAACLRDALVGAENYAAKVRGKKGPRERNLRDEALGALLRRE
                     MPLRMHAHRADDILTGLRIAEEFGLDYTLEHGTEGFLISSLLAAKGVRTAFGPAMTFR
                     SKLELREAHIRNVVTLVRAGVPVALMTDHPVIPIGYLRNEAAETIREGLSEEEALALV
                     TRTAAAHLGLEARYGSLEEGKGGDAVLWSESPFSPRSVAVATVVDGVVAHRA"
     gene            complement(17502..18434)
                     /locus_tag="PL162_01000"
     CDS             complement(17502..18434)
                     /locus_tag="PL162_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQF90637.1"
                     /translation="MEYLIPIMAAAVRSGTPILFATLGELLTERVGVMNLGLEGIMLM
                     GSLSGFWGALVFSNPWAGIALAVLVGMALSALHAVLCVSLGANQVVSGLALAMFGTGT
                     SALLGKNLVGVTIPGLPAAPLPGLAELPWVGPILFSHDVLVYGGFILTAFLAWFLYFT
                     RGGLNLRAAGEAPRVVDAAGLSAVRLRYLYTILGGGLVGLGGAYMSVVYTKMWAEMMT
                     AGRGWIALALVIFGLWHPVRSALGAYFFGGVGALQLRMQAGGTTVPAPLLMMLPYVMT
                     IAVLFVIAVRKGYAVSLGAPRSLGVPYHREERDG"
     gene            complement(18513..19583)
                     /locus_tag="PL162_01005"
     CDS             complement(18513..19583)
                     /locus_tag="PL162_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQF90638.1"
                     /translation="MPRLWLEKRKDIPWWIGGTLSVLSVILALGAGGGLLLALGVSPW
                     EAYVTMFRGALGNWYGLSETVVKAIPLAMAGLAVSLAFRMQVWNIGAEGQILLGALAT
                     AATVRYAFVDNFFVMLFLMMLSAALAGGLWGALAGWLKGRWRVNEIISTLMMNYIALH
                     TVDYFIYGPWRDPASLGFPMTAPFPPAARLPLFLDTRVHLGIFFALILAGALYFFFRW
                     TRWGFEVRVLGENPQVAHYAGIPYVRNVVLLMFFSGALAGLAGMGEIAGLQGRMQPGF
                     SPGYGYTAIIVAWLARLNPGAILGVAFFMGALLVGGDTLQVVLRLPLASTHIIQGCIL
                     IFLLGGDVWRTHRIRLFRDKKC"
     gene            complement(19576..21180)
                     /locus_tag="PL162_01010"
     CDS             complement(19576..21180)
                     /locus_tag="PL162_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQF90639.1"
                     /translation="MWYSGAVYAMPGKERRSAMTEAERTLVSMRGIVKSFLGVRANDD
                     VAFDLRAGEIHTLLGENGAGKSTLMNVLCGLYTPEAGCIFVDGKEHFFRSPSDAIAVG
                     IGMVHQHFLLVPVLTVWENMILGDRAIPSVIPRKKVCRRIEELSTQYGLAVDPEARIW
                     QLSVGEQQRVAILRALYRGARTLILDEPTAVLTPQEARALFSTLRRMAEEGHGVVFIS
                     HKLDEVMALSSRVTILRKGRVVGTWRAGEISKAALAEQMVGYQVPRSENPSPRGGEET
                     LLEGRGLVVQSDRHLPAVRGISLELRRGEILGLAGVAGNGQRELCEALVGLRPLEAGE
                     LTLRGRRVTGAPPRIFQRQGVSYVPADRMGMGLVGNMNLRENVALRHYWREEFSRWKE
                     ILRWDVLGAFTEELVRRFRVQTLSCDVAVRTLSGGNLQKLMLARELGLLPSILVVQEP
                     TWGLDVGATQYVRDQILEARNRGAAVLLASEDLEEIFALSDRVAVIHQGRLMGVSDNP
                     RDLGEERVGLMMAGTPLGAERGEGHA"
     gene            complement(21409..22494)
                     /locus_tag="PL162_01015"
     CDS             complement(21409..22494)
                     /locus_tag="PL162_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HQF90640.1"
                     /translation="MRKRDVWWGAWLLVGILATGAWAFDPVAPEELKPGFVYIGPVGD
                     GGWTYMHEQGRLAMEQAVPGVKSSIVESVPEGPDSARVLETFARNGSKLIFATSFGYM
                     DFVMDVAKKFPDVVFMHCSGYKTAPNVGVYFGRMYQARYLSGLVAGKMTKKNVIGFVA
                     AHPIPEVVRGINAFTRGVRRANPDATVRVVWLFSWFDPGKEKEATKALVDAGADVIAM
                     HADTGAAPQAAEESGVYVVGYNNDMSQYAPTKHLTAPIWNWGMIYTAAAQEVIAGTWK
                     STSQWPGLKDGMVGLAPYGKDVPEEVKALVEEEKAKIVGGTWDVFTGPVKDQSGTVRV
                     PEGTTMTDAEMLAFDWFVEGVEGEVPK"
     gene            complement(22624..23289)
                     /locus_tag="PL162_01020"
     CDS             complement(22624..23289)
                     /locus_tag="PL162_01020"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HQF90641.1"
                     /translation="MKNWVGFFDGGSRGNPGPAAAGAVLLDDRGDVVFEEGRFLGRRT
                     NNEAEYEALIMLLEEVDRRGFCKGVVLGDSRLVISQMRGEWKIREPRLRALAERARSL
                     VRGDVRFEWIPRKDNDHADDLVNEVLDREEERGRSAPPRPDRGELPEEKEEWRHGTLR
                     LVSDHIALVMGAPDEYVVNLAEGTCSCPGFQARGDCVHLREGMDAKRSLGAPGRGGSG
                     ASR"
     gene            complement(23301..25352)
                     /locus_tag="PL162_01025"
     CDS             complement(23301..25352)
                     /locus_tag="PL162_01025"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQF90642.1"
                     /translation="MEIILRYLNEVDRAKSPQRACALATTIIAEQTRYSRPAIFLYNP
                     RTDSLELTAYANHNPTAERITRGKGVCWRSFASEEIQVVNDVTKDPDYFQDMLDTQAE
                     LSVPIVWHGSVFGVLDVESSQRDCFSDEDVRFARLLAGILGSVLSHVETETQFRKNLE
                     RGKMFRANLIKAQEALNRALEEANAQKGAVEEHARIQRALIDVSRTLFVCRDARQLYL
                     LIMEILVRELGFPTVLVFGRDHREAPLSCQASLGIELDLEQESLLMGGSGIVGHVVKT
                     LEPYLCSDTAQDPYFDPDDYGTRSELVVPIRSGDFLWGLVVAIARRPGAYTLRDLETL
                     TLVAGFMSVVLENIANLDMLAREIKLMRFLHGMVHELSGERDNRSIALRIIALLREAF
                     DDMLVTVFDVREEGGRMEIFPMASSGSMEEAEGLWRDVVLRPGRLFQSTVAQAATSRK
                     VVSVPDTDQASGYVRGHSDTRSELNIPILHGERVRGVLCFESPRPRTFTQDDEELFVI
                     LARHLGALWAVNDLLDMTELKAMRDGLTGIWNRRFLFSYLEGDGEDLVRRGGTLTLVM
                     VDLRRFKLINDTYGHAVGDEVLTTVAAFFYKRLEGVGFAGRYGGDEFLIVLPDKTLEE
                     AERLVEELGQGVKELAFSVPELRTQANFGIASCPRDGVSIVEALRRADERMYEAKRRG
                     T"
     gene            complement(25455..26240)
                     /locus_tag="PL162_01030"
     CDS             complement(25455..26240)
                     /locus_tag="PL162_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQF90643.1"
                     /translation="MNRWSRGMGWVLWVMAVFAVGSSVAEGASPRSLQEIRARGELVV
                     GIEDDTWGRFHIWDKGSVEGLEVDLAERIAQAVGVSLRVMPIPWGKGEAGTISGSWES
                     GTWVEADLLLAGITTDAERAQKVTFSDWYFSAGQTVLFRKALGARTTGDLAGRRVSFQ
                     GGTTSEAVVRDSMPRSEAVPFATWSDAFSAFMEGTVDAVVVDSPLAIQKARENPELHV
                     LGVLLSRERYGVAMPKDVDPEFKALVDQVVRQCREELFRKWFS"
     gene            26385..27029
                     /locus_tag="PL162_01035"
     CDS             26385..27029
                     /locus_tag="PL162_01035"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HQF90644.1"
                     /translation="MTWYDLTHLITSAMPVYPGTEPPELLEATTLEREGFRETRLRLF
                     SHTGTHMDAPSHMIAGAPSLDSMAIGAFAGAAVKVDLSSFPPGRAVPRSFLEEALEGA
                     FTPDFVLLHTGWDRFWGTEEYFSPFPTLTPDGAAYLATRALKGVGVDAISVDPMESVA
                     FPVHRALLSRRLVIVENLRGLGELPPQGFTFLALPLHYADADGAPVRAAALLSA"
     gene            27067..28620
                     /locus_tag="PL162_01040"
     CDS             27067..28620
                     /locus_tag="PL162_01040"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015114 - phosphate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi symporter"
                     /protein_id="HQF90645.1"
                     /translation="MTSLSLILGGLGLFLLGLDDSTRTFREEWGPRSRRLFGRMSRST
                     GASFLLGTVLSALSQSSTAATALTIGLVNAGVLSLAGAVVVMMGASVGPSLMTFLISV
                     DVTSYAPLILGVAVFGERFTRGPFRQLSAVVRGLALILTGMALVKLGVVPLTARADLQ
                     ELFAAAGSSPWLLGLGVLVVTAGTQSSSLVVALAMVLLSQGLLPPEGALPVVLGSHVG
                     SSATVLLAGLGGRPQARALAWASLIYKIVGVGAALLLASPIQGVFASLTFPLPLRFAL
                     LQGIILWGNAALLLPLAPLLAQASQGVARLFARETPGTPLYLDDAALPFPSLALALLS
                     REMVRLASALEQLLTLTLADRSRPRDRSALQEGLPSLAEACADFLLSIPSGADRGVEA
                     SREELFDSLRSLSQLTTQLVTRFAPRSDALGYRLTDEGPDGVPWKDFASALVELVRHS
                     LGTFVLGEREPSPLARRSFQNFHVQENLLRARLRGSGEGRVTRETMEALEWVDEASSL
                     ARSAQELVS"
     gene            28653..29393
                     /gene="phoU"
                     /locus_tag="PL162_01045"
     CDS             28653..29393
                     /gene="phoU"
                     /locus_tag="PL162_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HQF90646.1"
                     /translation="MFFHRNSSTERLENYLYDRDRKRILLMVSAMGERTARALEEAVE
                     ALVTLDGPRAAAVVAQDGAIDALEGEIEQECLLSLAMRQPVREDLRFVFSVLKIVTDL
                     ERLGDEAANISEKTMALLGAPLFQPLVDIPQMGNLVAAMLRDSLQAFAQNDAPGALEV
                     IRRDDVVDTLYDKVFRDVMAQLIADGIAEEDRVRRGAALILISRYLERAGDHAVNIAT
                     RAYFMITGKRLPDRHEARRSPAPEKGQP"
     gene            complement(29471..30823)
                     /gene="gdhA"
                     /locus_tag="PL162_01050"
     CDS             complement(29471..30823)
                     /gene="gdhA"
                     /locus_tag="PL162_01050"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HQF90647.1"
                     /translation="MANEHYIDGLIAELKAKWPWETEFIQAAVEVLESVKPVVAQHPE
                     YEKYRIVDRMIIPERVIIFSVPWRDDKGEYQVNQGFRVQYNSALGPYKGGLRFHPSVN
                     LSILKFLGFEQTFKNALTGLPMGGGKGGSDFNPKGRSSEEMMNFCQSYMRELFRHIGP
                     DTDVPAGDIGVGGREIGYLLGTYKKITRDFQGGVLTGKNVNWGGSLIRPEATGFGAVY
                     FLQEMLDKKGDSIEGKTIALSGFGNVTWGTALKASELGAKVVTIAGPDGYIYDKDGIS
                     GEKVEFLLSLRSSNQDIVKPYADKFPGAQFVPGKRPWEVKCDIAMPCAFQNEMSGDDA
                     KALLSNGVKYVVEVSNMGCTPEAMETFVGNKVPFAPGKAANAGGVATSGLEMSQNSLR
                     LQWTESEVDARLHQIMKTIHRNAYDTAKDYGVEGNYVVGANIAGFIKVANAMVGQGVI
                     "
     gene            complement(30963..32270)
                     /locus_tag="PL162_01055"
     CDS             complement(30963..32270)
                     /locus_tag="PL162_01055"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HQF90648.1"
                     /translation="MAGEKGPIREEDRYLRYLEARACEAEVLAASQEALFNLVRELEK
                     CPSVSAVLDLLEHRLRSLLPLGPALLVMRDSPSSDVWRWRGAGANLREGMSLSPKDQE
                     ALRSLEGPASVLASQIPGLAPGGAESFLAVPVTQGGVLWGGVGGSPLPSGVFRDNERR
                     LAGLLALHLGLRLESMDHARRREEEAARLKKELALAAAAQRSMRPPEVLRWPEGVAAI
                     WTRPWGEVGGDACDLLRDSAGNVHLLLADAMGHGVAAGLLVTFLRGLFFSRLLRESLS
                     PKELASLLDEGLKSVAGDSDDAPFCTVIHGVWDPRKKVFSYLSAAGAPAFILRSSGEM
                     VALDSTMPLLGVLPVEPEGREVEGSPGDVVCFCSDGLLEALPLDESQSPRSLLAAWLQ
                     GVLREGDPSGVPALVQKEVSEGRWVPRDDATLVLLSLGAGGGG"
     gene            complement(32379..33500)
                     /locus_tag="PL162_01060"
     CDS             complement(32379..33500)
                     /locus_tag="PL162_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HQF90649.1"
                     /translation="MLYFQALSNLIAQSGFAGLTLGNVVMLLVSFVLLYLAIVRGFEP
                     LLLVPIAFGCLLVNLPLSGIMDEGGFLRYVFFGTEHEIYPIIIFMGIGALTDFGPLLA
                     NPITFLLGAAAQMGVFVALFLATALGFNIREAASIGIIGGADGPTSIYLTMKMAPQIL
                     GAIAVAAYSYMSLVPLIQPPVIRLLTTKEDRGIRMDNLRPVSKMEKVLFPIVCTVASG
                     LVLPMCVPLVGVLMFGNLLRECGVTERLNHATQNEILNTTTIFLGLTVGATMEAATFL
                     TVATLKIIFLGLVAFMASTAGGVLFGQVMKKWSGGKINPMIGSAGVSAVPMAARVVQR
                     MCQQEHPGNFLLMHAMGPNVSGVIGTAVAAGAMLSLLTN"
     gene            complement(33537..33971)
                     /locus_tag="PL162_01065"
     CDS             complement(33537..33971)
                     /locus_tag="PL162_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HQF90650.1"
                     /translation="MARNFRVTVNGKAYDVSVEDLGCGAMPPSAPAPAPAPAPVAAPA
                     PAPAPAPAPAPAPAPVAAPAPAAAPAPVAGGEVVASPMPGKILKVATSVGAVVKNGDI
                     LLILEAMKMENEILAGRGGTVKEIRVKDGDSVNTGDVLVVVG"
     gene            complement(34074..34445)
                     /locus_tag="PL162_01070"
     CDS             complement(34074..34445)
                     /locus_tag="PL162_01070"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HQF90651.1"
                     /translation="MNTASHFVGMGGAITLSLIAFSIVFLVLIALMGIIYANRYLAEQ
                     VDRRNREKAASKAPSGDGSGGRMAAAVALPDDGDLVAAITGALAMSLGRPVVVTGIRP
                     VRQSGSLWKALARSQNIEGLD"
     gene            complement(34470..36029)
                     /locus_tag="PL162_01075"
     CDS             complement(34470..36029)
                     /locus_tag="PL162_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HQF90652.1"
                     /translation="MSEKTIDQLCEQVLAKREAAKAGGGEKAVAKQHEKGKLTARERI
                     FKLLDEGSFTEIDEFVEHRCTNFGLEKTVFPGDGVVTGYGTIDGRIVYVYSQDFTVMG
                     GSLGEMHARKICKVMDLAVKNGAPVIGINDSGGARIQEAVDALSGYGNIFFRNVLASG
                     VVPQISVISGPCAGGAVYSPALTDFVFMVDQVGVMHITGPAVIKAVTGEDVTSEQIGG
                     AMTHNAKSGVAQFFSKNEDECFAEIRRLLGYLPSNNMEEAPGAECTEDPYAPNLELRN
                     LVPTNPNKSYNVISVISAIVDQGSFMEAQPLWAKNIVTAFARVGGIAVGIIANQANNL
                     AGCLDIDASDKASRFIRFCDAFNIPIVTFEDVPGYLPGTAQELGGIIRHGAKLLYAYS
                     EATVPLVTVILRKAYGGSYLGMCSKDLGADVVLAWPQAQIAVMGAQGAANIVFRKEIE
                     AASDQNAMRQQKIDEYEDAFANPYQAAKRGFVDRVILPEETRGAIYEALLLTENKKEQ
                     RPKRKHGVMPH"
     gene            complement(36121..36528)
                     /gene="mce"
                     /locus_tag="PL162_01080"
     CDS             complement(36121..36528)
                     /gene="mce"
                     /locus_tag="PL162_01080"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HQF90653.1"
                     /translation="MKPTVVDHIGVAVKDLRKALEFWENALGISCVGVEEVAEQKVRT
                     AFLPVKDTEIELLEPTADDSPVAKFIEKKGEGIHHIALRVENLEEALEELKARGVQLI
                     DEKPRYGAGGARIAFVHPKATGGVLLELSERSH"
     gene            complement(36652..37074)
                     /locus_tag="PL162_01085"
     CDS             complement(36652..37074)
                     /locus_tag="PL162_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HQF90654.1"
                     /translation="MEDRKIRVVVAKPGLDGHDRGAKVIARAFRDAGMEVIYTGLRQT
                     PEQIVETAIQEDADAIGISILSGAHDHYFRTIIGLLKAKGAEDIIVFGGGVIPEGDIP
                     GLMDLGAGAIFGPGTPTTTTIEWLQKAVEEKRARESRA"
     gene            complement(37154..38833)
                     /locus_tag="PL162_01090"
     CDS             complement(37154..38833)
                     /locus_tag="PL162_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HQF90655.1"
                     /translation="MFKEDQLQAIGKGAEAHRKTMEKMLAKAPERKKEFRTSSETVAP
                     VYSPADVENIDYMRDLGFPGQYPFTRGVQPNMYRGRFWTMRQYAGFSTAEDSNARYRF
                     LLEQGSMGLSVAFDLPTQIGYDSDHPMAQGEVGKVGVPIDSLADMEILFDQIPLDKVS
                     TSMTINAPASVLLAMYIALGEKQGVRSEELSGTIQNDILKEYIARGTYIFPPKPSMRL
                     ITDIFEFCSNHVPKWNTISISGYHIREAGSTAAQEIAFTLADGIAYVDAAIKKGQDPN
                     VFGERLSFFFNSHNDFLEEVAKFRAARRLWARLMKERFGITNPKAMMLRFHTQTAGCT
                     LTAQQPENNIARVAFQCLAAVLGGTQSLHSNSMDEALALPTEKSVNIALRTQQIVAYE
                     SGVCSTVDPLAGSYYIESLTDRLCQDAETYIAKIDDMGGMLTAIEKGYVQQQIQDAAY
                     AYQRAIETGDMVVVGVNKFQMEEDNSGRELLRVDERVGRRQAEKIAAVKAKRDNLRVQ
                     TTLDEIRAAARDESVNLMPRILDCVRAYGTEGEICGVLREEFGEYRENIVL"
     gene            complement(39181..40305)
                     /locus_tag="PL162_01095"
     CDS             complement(39181..40305)
                     /locus_tag="PL162_01095"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQF90656.1"
                     /translation="MEGDREKRFKVLIVDDVPENIHILMETLKDEFSLVAATGGEKAL
                     RLARGEAPPDLILLDVMMPGMGGYEVCTHLKADPKTAPIPVVFLTALSQEEDEAQGLK
                     LGAVDYVTKPFRPALVKARVRNQLELKHHRDRLEDAVRDRTRELELTREVIIDSLAAM
                     AERRDLETGGHIQRTRRYVQFLAERAASHPDFAGVLSPETIALFASTAPLHDIGKVGI
                     PDGILLKPGKLTDEEFRVMGYHTLYGYEILRDGERRLRGNTFLSHAQDIARDHHERWD
                     GKGYLRGLAGEEIPLSARIMTLADVYDALVSRRPYKEPFPHHVAMEIIAEGRGTRFDP
                     RLVDIFLASGDAFFQIFQEHADDSEGRPSGDGGEMDQEVI"
     gene            complement(40369..44370)
                     /locus_tag="PL162_01100"
     CDS             complement(40369..44370)
                     /locus_tag="PL162_01100"
                     /inference="COORDINATES: protein motif:HMM:NF017078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQF90657.1"
                     /translation="MTISRKIMLGLTAVVIFSLGIFAVRENFMVMESHRSLERDEARR
                     ALHRIEAIIQVEVGHLDTTCRDWATWDSLYAYVEDHSPEFRRENLHDGALQSSGVDLI
                     LLFDAAGALEEKLISPVFMKGPLPPEFLEGLYEALAPPCQGILKTPQGLIMVASRPII
                     RSDGHGVPRGTCVMGRVVDAEAVENLRRQSSSQVYIDPGEGKIFEELLRAEDGMALDQ
                     SRDDLLLGYALIKDISGVPVGVLRGEFSRPFHQAGKGMVRSSLVAILATGIVSLGAMM
                     ILLRHVVVRPLGELAAQLRAPRREGEGVAKLISDDRDDEIGVVARAFNALIADLEESN
                     EALKREVWHRKESEGNLRKLLRVTEQSPMGVVVTDREGSIEYVNPKFEEMTGYTFAEL
                     LGKSIESIDGDRPSVSPWEEVWETILAGRVWSGDYPNRRKNGEEYWEHASIAPVADGD
                     GRITHFVIVKEDVTLRLRSQEELRLFRLLVDSSGQAVAVADADRRLQYANTTFLRWVG
                     QNLETVEGRLLEDFCAPSSADRIRREVAVELETSGEWSGELTMGGPFGAAKEVLASFF
                     LVRDESAPCRTALVATNVTELKAQQEEIERARRAAESADRAKGDFLARMSHEIRTPMN
                     AILGMAHLMSTTALSHRQRDYLQKSQGAAEALLGLINDILDFSKIEAGKMTLESVPFD
                     LDTVFEHLATVTALRAQEKGLKLLFDPRPDVPRSLEGDPLRLGQILVNLVTNAVKFTE
                     RGEVVVTVALEDWSPKVVVLSFAVRDTGVGISEDKIGALFQSFSQTDGSITRRYGGTG
                     LGLAICKRLVEMMGGTIEVSSTPGVGSTFEFSALLELGKAQEAPLSPPEELRSLKVLL
                     VDDNAVARGVISRMLTSLSFEVISTSGVEEALETLAEDRGAPVRLVVADGDLRAVADG
                     TLHRALQELPSGPIPLVSLVSLAEDRKKSKGGKKTREEAVIKPVLPSSLLGAVLAALG
                     RDDLRLRERTPSPRGGVSERWEGTQVLLAEDNDINRQIAREILEGRGVVVTVATNGRE
                     ALEAASRREFDLVFLDIQMPEMDGLEAARRIRALGGAWETIPLVAMTAHAMERDRGLS
                     LEAGMNDHVTKPIDPDGLFETMERWILRPEKKVLSASGPEEVAGAAPPPFAGGVDLAR
                     GAERLGVDEAFYRHLLRLFVEEQIDLGIALKGSGAEPSDELRRRIHTLKGTAGNLALD
                     DLAAVAGALERALDSPSSEVVEEAKDNVLEEWRAVREELVALLGEEVEGDPEAEPMGL
                     LEDREEARRLLEEGRALLDDDLPKAEARIAEARRRAGREGEDLFQTLDRMLRHFDTDG
                     AASVLDQLVKLLS"
     gene            complement(44489..45079)
                     /gene="pyrE"
                     /locus_tag="PL162_01105"
     CDS             complement(44489..45079)
                     /gene="pyrE"
                     /locus_tag="PL162_01105"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HQF90658.1"
                     /translation="MGILRAMNGLEDIMKETGALLSGHFLLTSGKHSDHYMQCALLLK
                     HPRHAAFVGEGLARLLAPLGPQMVASPALGGVIIGHEVARALGVPFVFCERQEGRMVL
                     RRFPHPGAVRFVVVEDVVTTGGSLREVGEHLATGGAEWVGSACVADRSGGKTGLPHDL
                     TSLLVAAFPLYDPEECPLCAAGLPLVKPGSRKGPGE"
     gene            complement(45081..45782)
                     /gene="pyrF"
                     /locus_tag="PL162_01110"
     CDS             complement(45081..45782)
                     /gene="pyrF"
                     /locus_tag="PL162_01110"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HQF90659.1"
                     /translation="MQRKAEVILALDTRTLEEGQAMLDRVGRGLRHVKIGPRLYALGG
                     RDFIEAVQSRGYDIFLDLKLHDIPNTVALAVDVFARMGLWALSLHSAGGREMLSQARD
                     ARDGAESVMKLLGISVLTSLDDDLWGEVCPAGGGVRGAVAARARLCAAVGLDGLVSSP
                     QELTLVREEGGDRLLKVVPGIRPQRGEDDQRRTASVATAVRDGADYLVMGRPLLEAPD
                     PEAALAAVHREIEEA"
     gene            complement(45832..46755)
                     /locus_tag="PL162_01115"
     CDS             complement(45832..46755)
                     /locus_tag="PL162_01115"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HQF90660.1"
                     /translation="MRKRSVQTRVGGVILDSPLIVASGVWPLERDLWPREPLPFVGAA
                     CTKGITLEPREGNPGVRLWETPAGVLNSVGLQNGGVRSFVERDLPEMAREGVPLVANV
                     APLTEEDFDPLFGALEAGAEHLAAVELNLSCPNVACGGAAWGVTAKGVSRAVALARSR
                     WRGPLWVKLTPQAADLGATARAAEDRGADAVVVANTWLGMAFDVERHRPALARVVGGL
                     SGPAIFPLALRCVWDVAGSVSIPVVGCGGVTTGNDVISMLLAGASAVEVGTALMADLG
                     AAKAMMEAVEGHLERFGLDTISSLVGAGRRA"
     gene            complement(46752..47543)
                     /locus_tag="PL162_01120"
     CDS             complement(46752..47543)
                     /locus_tag="PL162_01120"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90661.1"
                     /translation="MSRESSLEERALVVGGRPLIPGLGEVILEAPRLARELRPGQFLM
                     ARVSPWQDPLLGRPLGVSAVRGSQITLMVAALGRGSRALLALRPGAEVTLRGPLGTPF
                     PPPAQAPLVLAGGGFGVAPLMYAWDELRAGRGEVPTVVLGVPDGSWAPLERLVAHRVG
                     KHLRVFSDDGSLGERGTPCDALEGAGEVWACGPPAMIRAMARCAPPEMRLLVNLDQRM
                     GCGYGGCLGCTVPTVRGPVRACVEGPFFDARDVDWGALEELEGRS"
     gene            complement(47536..48843)
                     /locus_tag="PL162_01125"
     CDS             complement(47536..48843)
                     /locus_tag="PL162_01125"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HQF90662.1"
                     /translation="MILLKNARLFDGVQLHGGATDLLVEGKTVTRIASGLSGSEGARV
                     MDLAGRVVAPGFVDLHGHFRDPGQTWREDLTSGARAAAAGGFAVAVAMPNTEPPLDSE
                     ALVRDVRERGRHTGGALILPAGCVSRGRSGEEMAELASMVEAGAVLFTDDGAPVLRSS
                     LLRHALLYASDLGVRIMEHPEDPDLSKGAQVHEGRCSALSGMRGMPAAGEVIGVGRAV
                     ALARETSCAVHLTHVSTAQALEEIRRAKGEGLPVTCDVTPHHLIFSEENVLTSHLSAI
                     FKVNPPLRSLGDVEALWEGVADGTVDALGTDHAPWHEDEKDLPFVEASFGIASYECAV
                     AAVLDEGRRRKVPLEALLRLWTSGPAALLPPWAGVPGRIEEGGGAFLTVLDLDLPLEV
                     NPDQWRSKVRLTPYRGKRFIGAPVATMVAGALVFDALSEPPHE"
     gene            complement(48840..49769)
                     /locus_tag="PL162_01130"
     CDS             complement(48840..49769)
                     /locus_tag="PL162_01130"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HQF90663.1"
                     /translation="MAWSHRNLLDVDVLTREDLETLLDQAMHMEDLMDRPVKKVPALR
                     GKLVVNIFFEASTRTRVSFELAEKFLGADVVNWASSGSSVSKGETLRDTVWTLEAMGA
                     DAVVVRHSMPGVPWYLRDKLRRASVFNAGDGAHAHPTQALLDVYTAWRHLGSLEGKRI
                     AIVGDILHSRVTRSDIIAFRTMGASLVCSGPRSLMPKDMGALGVEYEPDARRAVVGAD
                     AIYLLRIQMERQMAGLFPSEDEYHRLWGANEELVRRARPGALVMHPGPINRGVEIESR
                     VADGPQSVILPQVKSGVATRMALLYLCMGGGEA"
     gene            complement(49773..50375)
                     /gene="pyrR"
                     /locus_tag="PL162_01135"
     CDS             complement(49773..50375)
                     /gene="pyrR"
                     /locus_tag="PL162_01135"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HQF90664.1"
                     /translation="MNWKEKARVMESPDMARALKRMAHEIVERNKGLEGIVLVGIQRR
                     GVPLASRLGALLEGFEGMRPAQGELDITLYRDDLSLRYSQPLVRRTDIPGGVTHRRVV
                     LVDDVLYTGRTVRSALDALMDLGRPAGVQLAVLVDRGHRELPIHADYVGKNLPTSSSE
                     VVEVRLQEVDGVDEVWICEKVEKVEEGPERPLMRFAREGE"
     gene            complement(50642..52081)
                     /gene="proS"
                     /locus_tag="PL162_01140"
     CDS             complement(50642..52081)
                     /gene="proS"
                     /locus_tag="PL162_01140"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HQF90665.1"
                     /translation="MAKNITKRDEDYSEWYLDVIRAAELADYAPVRGCMVVRPTGYAL
                     WEHVQEQFDRRFKETGHVNAYFPLLIPHSFLEKEAKHVEGFAPECAVVTHAGGEELEE
                     PLVVRPTSETIIGHMYSKWVQSWRDLPILINQWCNVMRWEKRPRLFLRTSEFLWQEGH
                     TAHATREEAVEETLRMLEVYREVMEDELALPVIPGEKTEGERFPGADNTYTCETMMSD
                     RKALQAGTSHFLGQNFAKAFDIQFQSEAGQLEYAWTTSWGASTRLIGALVMTHSDDDG
                     LVLPPRVAPTQVVILPLSNDQGRMDEILLPRAREVEARLNGVLGPRRVLIDTQFHLRP
                     ADRFFHHLQRGVPLRLELGERELEEGTLRYVRRDTGEKGSFPLDRAAEEVPALLGRIQ
                     RDLHAKALKFRQDNTHEVSSFAEFQEVLASSGGFITGYFGGSKEDERAIKEATGATPR
                     CFPLADQRTGKCFYTGQEGARRVIFAKAY"
     gene            complement(52142..54223)
                     /locus_tag="PL162_01145"
     CDS             complement(52142..54223)
                     /locus_tag="PL162_01145"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQF90666.1"
                     /translation="MGWRAMKGFLALGVGLLVLGGAAWGEPGGVVLALSGGGTKGFAH
                     VGVIQVLERAGIPVAGIVGTSMGSIIGGLRAAGYDGASLEEVVRGIDLPALLDDRNAS
                     SSANGEVHSLFRRTFDRRGRSTGPLGIMEGKHLLSTLHQLTAQVREGNFDDLPIPFVA
                     VATDMETGETLLIRRGSLASALRASMAIPGFFEPWPVEGRMALDGGLVANLPVHIAKE
                     VFPGYPVVAVDVSSALQPASRLRNAVEVFDQSTAIMTRQGYLDNLRAADLVIRPAVGD
                     RPMLESGHVGEIVALGRQAAEQELSGLLRLAAAAPPVPPRPARADRTVAAVRVEGLSP
                     VTGAFLEHSYSSWVGRPFDAVAVLDACETIKERDDVGVVEYRLEDAGVGQVAVVLQVH
                     PAPDVEIRVGGYTSNLAPERWLAVEGVQRGVWAEGDILTSELRVGEDWGAGLHYFAPV
                     SGFRRMEYYLEARSETVTPHGMDREAWDRYVAGVRRFFWRDGMEIGWGIVGERVEYGG
                     ETQSAWGPKVSLSWSTLDNPDDPTEGVRFQGNLWWSNLDALLWTADMLWVQPWGAQSR
                     LLLSGGVQGGDASSPAHAAYLGGPKDLFHLASTPWRGERSAWVRLGIRRMLWESVLLG
                     PIYGDLFVTRGALFDEDWDEVASPWEAGLALAVPGKLLGGRVLVIYDGDGNVTFGFEL
                     GSDFRRREIIP"
     gene            complement(54562..55680)
                     /locus_tag="PL162_01150"
     CDS             complement(54562..55680)
                     /locus_tag="PL162_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90667.1"
                     /translation="METGAAQTSSLLDLLSYLLGVPQEEVSRLVAEFSSDGGADNPRN
                     LFSFLAAQTRRYHVLWVVSPFTDQLSILAVAVMGPGREVVWWASTAVPHGRDSATFRL
                     WHRVIEVASGKASLGSTNPAESEVVRAQEVARKYGVPWPGLAAGIVPELESAMAEKGS
                     DQEDDDGYFEDSGMTRRFSAVIEQYLRESLGKVSPLTALRLSRVSLPDLRDLGLEVAA
                     LQQSLDKFLGFHLNADLSSKEGGAEALGGEGLGSLILPSAGVRELDPVVDPLGGVPLS
                     SVVPGDMMSLQEIGGSPIPGRVYLIRVMRDGQFEIYGHLGGDKGFFKAVAPGDIKIAL
                     SPYDEEKDKPPLSERALLWGAVGLVGLMVVLLFYFLWG"
     gene            complement(55949..56440)
                     /locus_tag="PL162_01155"
     CDS             complement(55949..56440)
                     /locus_tag="PL162_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HQF90668.1"
                     /translation="MSQQILSKFVNANGAALVSVSSTLGVKAELQKSHIAQGVNTPGS
                     RVAAVVGIVGGGVHGTSSIMADEGGFAAYVNAMGGGMISADLEDPVALSVIAELTNMV
                     NGQTLMALDIDGVDLTPPQVITGENIKAVPPQKPAIKSFTLPFSVVPSGTVFLVISIY
                     QEE"
     gene            complement(56737..57183)
                     /locus_tag="PL162_01160"
     CDS             complement(56737..57183)
                     /locus_tag="PL162_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HQF90669.1"
                     /translation="MIILGVDPGRGKFGWAFGSEEGTLLFAGITPTAARDAWGARVRE
                     GRWDALLPWSTEGHAEAVRSFPEHFVLGDGTARHPFLALMEGYGWAVTLVGEENSTLE
                     ARRLYAQIHPPRGWRRLLPAALFPPSRDLDDLAAWELVRRFLGEAP"
     gene            complement(57180..58058)
                     /locus_tag="PL162_01165"
     CDS             complement(57180..58058)
                     /locus_tag="PL162_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQF90670.1"
                     /translation="MGSDVTWRALGVVRRAFEALPPDWALFGGGVLGGLVWALDKGRV
                     DGAEARCVGALGVGVTRARRIVRASYVNLGRSVVEFLLIPKRGERWRSRVAVHGEEYL
                     RRALEGGRGVVLLTAHFGSWELAAATLAARGFPMNAIGAEQRDPRITDLIISLREAAG
                     VRTIGKGFDLKGALRCLRRGEILGVLLDQDARSAGIRVPFLGHPASTPYGPVKMAAKM
                     GSRVVPLFMVRRPDGISHDLHLLPALEGKDGLPFGQDEEYSVALCNEVLGTWIRRHPD
                     HWMWLYPRWASTQALP"
     gene            58155..58955
                     /locus_tag="PL162_01170"
     CDS             58155..58955
                     /locus_tag="PL162_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQF90671.1"
                     /translation="MLRRILHPIDLSPQSLEGAKWILDSLATPRTSLLLVHVVNPEAG
                     LETPGLVRDAEEHMGPLEEELQALHGGVSSLVLAGDDEEVLAEVAHREKCSLVAHIIH
                     PEDDPLPLIRPIPLPHLLIPLPDDGTLPAGDLFSRMVVATDLAPDRTDALLESLRRVA
                     EGRARQTVLLHAVPLDDPGAAEELVMAADEALQAVKESLLPWGGEVTARLVSGDPNEE
                     LPAVLEDLAPSLLVLGLSRHGEMWGLLVGSTAEALIGAAKSPVLIIPA"
     gene            59125..61236
                     /locus_tag="PL162_01175"
     CDS             59125..61236
                     /locus_tag="PL162_01175"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQF90672.1"
                     /translation="MHRSRRGVSLKFQILSGVALIVLGVLAASFWGSYLTARDRLMES
                     FVAQESGAVSGLAQATDKTLELLNQACVSNLDVAASTLTQGNIIARPPGAVGTWQLPQ
                     LLLSCGGKVTKLTGNNDLPEEWSRRMRGVFSLFQVASSDGKSALVRVATSLRDGQGNS
                     MVGTVIPEDSPVFQSVVTEGKEFPGTTTIQGEPYFASYRPLPGRDPQDFIVISSGTSL
                     QPLVKLIRETHIGEGSQALILDGEGRPLGGADPGAAEEEIGAVLRENGRGQTPGTVWS
                     FAVGSGSEARQVFAFRTEDPSWTVALSLPSSVILAPLGAMRGRMIFWGLLSLVVGMAL
                     AAIVLRRLLAPLRSVIDTAERVAQGDLRACSLGQEESRNEIQVVLGAFSRISASFRDL
                     VLRMRRIQELSQERGDAMEQINVEVADSLREALAAAGDMVRTVGDVAQAAESTKARVA
                     SVTRSSREAAELVQDLSRKAETVAREAASDGDAVRSVAAEIAEAGKASTRVRNALGAL
                     EHSVESISRFVSTIRGIADQTNLLALNAAIEAARAGEAGRGFAVVAEEVRKLAEESNE
                     AARQIQSVIEEVGTRTDETVRDTDGAIELISKTVENSTGAAERIGKIMEDVSDIAKGI
                     QSIAQATQGQLAGNEAVEGAMDSVHLQMSEGHRAAHRVEEGSRQVAAQMERLEEIRQG
                     QQEVLDDLEEILQGYVLDEGP"
     gene            complement(61328..62596)
                     /locus_tag="PL162_01180"
     CDS             complement(61328..62596)
                     /locus_tag="PL162_01180"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HQF90673.1"
                     /translation="MDLRAFLRAEVKPALGCTEPGAVALAAATAAKEFSGPLEGIRLR
                     LSANVFKNGHSVGIPGTGGLQGNRLAAALGALAGDASKGLRSLEGLPSEAVERAQKLV
                     ETGNVTEQVVPDVPSVYAEVELRGPEGTAGAVVSGSHDRVAEVRKNGKVVRRLLEADT
                     SVQGRPAYLEELMRLTMEDLWRLAGTLDGEMEIFLLDGARTNMEVGEAGLQGDWGLGV
                     GRVLQERGTPGDLLWEIKATASAAADARMGGVPLAVMSSAGSGNHGITAIIPLVVAAR
                     HWGSPFRSLAEALALSHLVTGYIKAHTGRLTPICGCAVAAGAGAAAGLVRLAGGTAAQ
                     GDRAVASLVASLVGMLCDGAKGSCALKVGAAAGEAYTSALLALENCGVGERQGVVYAT
                     LADTARAVGTLSTLGFSSADSVMLRLLGEP"
     gene            complement(62620..63792)
                     /locus_tag="PL162_01185"
     CDS             complement(62620..63792)
                     /locus_tag="PL162_01185"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQF90674.1"
                     /translation="MKRRDLGLSALATYLVQAYVAVFFLYPVALQQEGIPLTLVGGLL
                     GAYFGATTLGRPLGGVMVERLGVRWALILSGVGLVLTTTPMIWVRSLGPLLLLRGGMG
                     LFYGVAMVAVTAYQTAAIPAAVRGQVFAWIAVAYVLPQITVMPLGDFFLSRGGTELYL
                     ALAPVLALGCLGASLFSSDLPPATATPDQGWGSWGELLRVRPFYFVLASGAVFSLVNS
                     STLQYLSAAAAEKGLSSSAFFVANAASALAMRLLGSRVLDRFPRGILSGVMIALMGGV
                     LVLFPRGDTHLFLGILGLVYGVGMGFGFPIQLALAPDFVPRHLLPKGVAGSFFVMDLA
                     WIVTTVGAALLGTRWGMGGTLAGVGILGLGGGFLVAGAAWRLDKRRAGLRDEGKKD"
     gene            complement(63863..65044)
                     /locus_tag="PL162_01190"
     CDS             complement(63863..65044)
                     /locus_tag="PL162_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HQF90675.1"
                     /translation="MGESRDDIYREALEAHRRHRGKISVESRMPVDNFHDLSLAYTPG
                     VAEPCRAIEKNVAQVWELTNRGNCVAVVSDGSAVLGLGDIGPEAALPVMEGKCVLFKR
                     FAGIDAFPLVVAERDPDALVDLVARLAPSFGGVNLEDISAPRCFDVEARLQDRVSIPV
                     FHDDQHGTAVIVLAGVLNALKLEGKVLETARIVISGMGAAGTAIARMLASAGARHLVC
                     CGRRGVLDPEDPQASPVQRLLARETNPEGVRGDLAQALRGADVFIGVSKGGTVTREMV
                     ASMAPGSAVFALANPVPEIFPEEARAGGAAIVSTGRSDFPNQVNNCLGFPGIFRGALD
                     AGALRITEGMKRAAARALAEMVGDGLTREYIIPSAFDPEVVPSVAAAVARAAREEGVC
                     R"
     gene            complement(65103..65696)
                     /locus_tag="PL162_01195"
     CDS             complement(65103..65696)
                     /locus_tag="PL162_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HQF90676.1"
                     /translation="MISRPETPFRMTTPLVPEALRGLRCGDAVLLSGTIFTARDAAHR
                     RMVEVHLRGEPLPLDLRGQVIYYAGPSPTPPGRAVGAIGPTTSGRMDPYTPLLLELGL
                     RGMIGKGRRSAEVVRAMVSFGAVYFGATGGAAALLARSVRRVLPVAYDDLGPEAITAL
                     EVEDFPVTVVVDLRGHDLYDEGPAAFLESLKGSGTGA"
     gene            complement(65693..>65977)
                     /locus_tag="PL162_01200"
     CDS             complement(65693..>65977)
                     /locus_tag="PL162_01200"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HQF90677.1"
                     /translation="GNFETAPRAAKRALLRPCGDPHPDPRYAALERKILEEINALGVG
                     PAGYGGSITALAVHVEWLPTHLAGLPTAVNICCHAHRHASGELGGEERGS"
BASE COUNT        11817 a        22998 c        19492 g        11570 t
ORIGIN      
        1 aagggcgcct tcttccagtt cctccaccga tcggggaggg gcgatctccg ggggctcggg
       61 agcgccgcac tgcttggtgg cctccccaac gatccgacgc catcgctccc ggcgggccga
      121 aaggcgctct ccccccagcc ggggcacgga ccggacacac aggaggggga tcaccctgga
      181 gactcccacc tccgtggcca tgcgtagcag ggcgtcccag gcgtcttctt tgagcacccc
      241 cgccagcaac gtcaaccgca ccggggatcc tcggtcgctg cgtcgggact cctcccagcc
      301 gtaccacaca tcgccccgac gttccaggcg cagctccacg cgataccccg gcaagaggcc
      361 ttccacccgg tccccggagg cacaccgccg caccatcagg tggtggccct cctcggccgc
      421 caggcgccag cgcccgtcag gctcccgaag gctcgcttcc agcctcaggc gcaacggcaa
      481 ccccccacca ctcccctcga gatcgctctt cctgcacgtc gaagcgatgc tcccgcagcg
      541 cctcgaggaa ccgctcccgc tccttctgca ccatccccga aaaaatagcc cgacccccgg
      601 gaaggagggc ttctctcagg tggggaagca tggtcaagag gggctccagc aggatgttgg
      661 ccaccaccag gtccacccgg tggcgaaagt ctttcagcag gtctcccacc tccgccgtca
      721 cgacctctgg agggacggcg ttgaggcgac aattttcctg tatctcgggc atcacggcgg
      781 gatccagatc ccgggccacc acgctccggg ctcccagtcg ggccgctgct atggctaaaa
      841 tccccgaccc cgttccgatg tccgccacgg ccttgccctc caagggcgtc gattccatca
      901 gttccaacat gatctgcgtg gtctcatggt accccgtgcc aaaggcgctc cccgggtaaa
      961 tgtacagggg ccgacgcccc gggggctcgg ctccccggtg ccacggggcc atcaccacca
     1021 gggtcttgcc cacctccagg ggaggaaagg attcttgcca cgcgacgtgc catcgctgat
     1081 tttccacctt gcccacgtcc accaccgaaa tgcggggcca gggctccagt acttcgttta
     1141 cgcgcttgag ccagaacgcc acatcctgag aactccggta aaaggcccgc acccgacagc
     1201 gatcggtgcc ttcctggtgc tccgccccga tgctccccga caggtccacc agggattcca
     1261 gcacttcgcc gtccccatcc cgtccctcca gcgtaacgta ccaccagaag ctctcctcga
     1321 cggacacgag agttccccct ttcttttcgc ccaaggctcg aaaagggaaa gtcccctctc
     1381 tcccggcggc atcaggggca ttgtaccact aggaaaaggc ataaaaaagc ggcccctctc
     1441 cctaaggccg ggaaaaattc cgatccgtcg cggcgccgaa gcagatcgta acccgtgagg
     1501 acgcggggct tctaaaagaa aaaactcctc ccgacccgga ggtcgggagg aggggtaatt
     1561 cgacccccta cccttcgcta cggccgaacc accctttgat cttttccaga aggccctcgt
     1621 tttcctccac ggggacatcc atctcctggg ccagggccac cagcaacccc ctctgtcgct
     1681 cggtgagctc ccggggaact tccaccttaa tgtgggccaa aagatccccc cggccgctgc
     1741 tccgaaggcg gggcatgccc ttgccccgga ggcgaaccag gtcgccgggc tggctcccgg
     1801 cggggatggt cacccgctcg acgacgccat ccagcatgac gatctccgcg ctactcccca
     1861 gggccgcctg ggggaaggcc accggaatct gcacgtggag cgtatccccc tctcgaagaa
     1921 agcgagggtg cgtcgccacg gtcaccacga ggtaaaggtc ccccgagggg ccgccattga
     1981 cgccgctttc cccttcgccc accatcctca gccgggttcc cgtatcggcc cccgggggga
     2041 tgcgcacctc cacccggtgg cggtgccggg tgcgcccgtc gccgccacag cttcggcagg
     2101 gggcggcgat ggtcttcccc ttgcctccgc aacgggtgca gggcaccacg gaaacaaact
     2161 ggccgaaggg ggtccgttgg gcgtgctcca cctgcccccg acctccacag gccgagcagg
     2221 tttccgccga ggtcccgggt tccgcccctt ggccgctaca gcgcttacac gtctcccacc
     2281 gaggtatctg cacctccttg ggaacgccgg agaaaacctc ctccaaggtg caggaaatct
     2341 ccatctccag gtcggcccct cgccgagggg cgttgacgtt cctcgtcctc cctcgccccg
     2401 caccgccgcc cccgaagaag gagtcgaaga tgtctccgaa gatgtcccca aagggttccc
     2461 cgccgccgaa gggccccgcg cctccccccg gcatgtctcc cacgtaaccg aactggtcgt
     2521 actgggtgcg cttctgggga tcgctcagca cctcgtaggc ttcgctgatc tctttgaagc
     2581 gactttccac ctctcggttg ccgggattgg cgtcgggatg gtatttgcgc accaacttcc
     2641 ggtaggcttt cttgatgtcc tcggcactgg cgtcccgggg gacgtccaag atctgatagt
     2701 agtccttatg ggttcccggc accgtggctc ccccgcttcc ctgtggcgtg gcttctcacc
     2761 gggggacggc ttccgccgtc ccccgaacgc ttctcacccc gggtcatgat gggggcatca
     2821 cgcttccttg tccgaaaatt ctgcgtccac cgtctctccc tcccccgaag ggctctcctt
     2881 cgggccttcc gggtggggag cctcaccgcc ggactgatcc tgggcggcgt agagcttctg
     2941 agaaagggcg tggaggctct tggtcagcgt ctctatggct tgtcgggtcc gggccaggtc
     3001 atctttagaa gccgcctcgc gcaggtcggc gatctcctgc tccacccggg ccctttccgg
     3061 agcctccacc ttgtccccca ggtcttttag ggtcttttcg gcgttgtacg ccagggaatc
     3121 ggcctcgttg cgggcctcgg cggcctcctt gcgctgccga tccgccccct cgttcatctc
     3181 cgcctcgcgc ttcatgcgct cgatctcttc ttcggtgagc cgagaggatt ggatggtgat
     3241 gtgctggctc ttgcccgttc ccttgtcctt ggccgataca ttcaggatgc cattcacgtc
     3301 gatgttgaac gtcacctcga tctgggggac gccccggggt gcggggggaa tgccgtccag
     3361 gttaaactct ccgagcttca cgttgtccgc cgccatgggg cgctctccct gaaacaccat
     3421 caccgtcacc gagggctgac tatccgccgc cgtggtaaaa acttggctct tggaggtggg
     3481 gatggcggtg ttccgatcta tgatcttagt aaatacccct cccagggtct ccagccccaa
     3541 agagaggggc gtcacgtcca ccagcacgat gtccgtgcgc tcccccgcca ggatggaccc
     3601 ctggatggcg gcccccatgg ccacgcactc gtcggggttc acgcccttgg tgggctcctt
     3661 gcccaaaagg tcccggagcc tcttctggac catgggcatc cgggtggatc cccccaccag
     3721 gaggaccttg tccaccgcgc tggcggcgag ccccgagtct tccagcactc gcttggtggg
     3781 ccccacgacc cgctccagaa ggtccgccgt catctcctcg aacttcgacc gagagatgga
     3841 gagctccagg tgcttggggc cattctgatc cgccgtgatg aaggggaggg atatggtggt
     3901 ttcgggcatg gacgagagct ccaccttggc cttttccgcc gcttcccgca cccgctgcat
     3961 ggccatccga tcccgggaaa ggtcgatgcc ttcccggctc ttgaactctt tcaccatcca
     4021 ctccgccacc cgggtgtccc aatcgtcccc gcccaggtgg ttgtcccccg aggtggccag
     4081 cacctgaaac acgccttccc ccacgtccag gagggaaacg tcgaaggtgc ccccccccag
     4141 gtcgaagacc aggatcttgt gctctccctc tttgtcggcc ccgtaggcga aggccgccgc
     4201 cgtgggctcg ttgatgatcc gcagaacctc cagccccgcg atgacccccg cgtccttggt
     4261 ggcctggcgc tgggcgtcgg tgaagtaggc gggcacggtg atgacggcct gggtgacggg
     4321 ctctcccaga tactcctccg catcgcgctt cagcttctgc aacaccatgg cagatatttc
     4381 ctggggggta aagcccttgc cccccatggt caccttggcg tcggagccca tcctgcgctt
     4441 gatggacatg atggtgttat ccgggttgac gatggactgg cgcttggcga gctgccccac
     4501 cagacgctct ccctccttgg tggtggccac caccgagggc gtggtgcggc tgccctcggc
     4561 gttggcgatg acggtgatac cgtctccttc tcggacggcg acgcaactgt tcgttgttcc
     4621 cagatcgata cccacaactt ttgccattct cggtcacccc attttgagtt tcaaaaaaat
     4681 acttcctgga aatacccgca tcttttcaag aacttccgct tatgaaaagt ttttccccaa
     4741 aggtctaccg ggagccgcca tcgctcgcag gggtgtgggc ccccacccgc accatggcgg
     4801 ggcgcaggac ccgcccccct agggcgtatc cgggacggac cacgtcgatc acctggtggt
     4861 cttgggcggg atctttcacg ggcaccgtgc ccaccgcgtc gtgccgagcg gggtcaaagg
     4921 ccccttccgc ctcgacgagg gatactccca attcctgcaa cgcctcgaag aacaaccggc
     4981 gcaccatggc caccccctgg cgcagccctt ctcctccccc tgaggattcc atggcccggt
     5041 ccagattgtc caacacgggc aaaaggagcg tcaccgcccg ttccgccgcc aattctcgct
     5101 gcctgagccc ctctcgctcg ctgcgctggc gaaagttata aaagtccgcc cgggcccggg
     5161 ccacttcctc ccttagggca tttctctcgt cttccaattg ggccagctgt tcccgtagca
     5221 cggcgagatc tccgcccctc tccatatcct caacttcccc caaagcccct tcgttcaccg
     5281 gtccttcttc catgtctttt accatgcttt ccgcccctcc gtacgtctca cggcacttcc
     5341 tgtgccgccc cggccttttc cggcatatcc tggagccccg cgagcatggc ttccaccacg
     5401 gcgatggctc cctcgtagtt catgcgcacc ggtccgatga gccccaccac ggccctttgc
     5461 cctgcggagc tcaccggggc caacaccagc gagcaatcct taagggcttc cacggggttt
     5521 tcctcgccga tggtcaccga cactccctgc tcttgggcac aacgctccac cagatgggcc
     5581 atggatcgtt cctcctccag gagggaaaaa attgcctgca ggcggccgat gtcgtgaaaa
     5641 tccgggaggt tgagcaggcg gcgcgcccct cccatgaaga tcctggtggg ctgggaggaa
     5701 aggatcccct cgagctccct cagggccacc cggcacgcca tttcccgatc tcgaagctcc
     5761 gctaccacgt actgaataag gtcgcggcgc acgtccttcc aggggcgtcc caccgccacg
     5821 gcatggatgc gcgaggccag gaggtccagt tcgtcctggc tcatctcaca gggtaaggtg
     5881 aggacccggt ggtgcaccaa tcccccatct aacaccacca ccgccaggac tcgggtgctt
     5941 tcgagacgga cgaagtccag gcgcaggagc cccacctcgt ccaggggcgc cagcgtggcc
     6001 actcccatgt aggaggtcac ccgggacagg agatgggaga tgtgcgacag cacgtcctcc
     6061 agcccccggc gctggctccg cacctcggag atccacccct ccgcctcggg gggaagctcc
     6121 cggtggcgat ggagaatgga gtccacgtag aggcgatagg cccgggcggt gggcacgcgc
     6181 cccgccgagg catggggttg ttccaggaag cccatgtcgg ccagatcggc catctcgttg
     6241 cggatggtgg cggcgctacg cccggtgagg tgccgtttgg acagcacccg agatccgatg
     6301 ggatctcctc cctggatata ctcgtagacc accgccaaca ccacctcaag ctgcctctcc
     6361 gaaagcacgt ccccacctcc ttcccgtcct attagcactc ttcacgagcg agtgccaaga
     6421 gctttttctt accgtagact accaacggca ccgccgattg tcaagacagg tcaaattttt
     6481 ttctgcccta cacctttctc tccggaggaa agggaagctt ttcccttcga aggatctcct
     6541 ctccgtgcta aaatgcaacc caggggacgg cagtcctcga ctccaccggg gagaggggaa
     6601 aaagacccat gggtgagcca aaacgcaccg agatcatcct ggtacgccac ggcgagtgcg
     6661 cggaaaatcg agagggggta ttccggggac ggagcgacgg gccgctcacc gagcggggag
     6721 tgcgccaagc ggagctactg gcggaagaag ttgcccggtt tttttcccct tcggccattt
     6781 tttccagccc cctgcagagg gcccggaata ccgcccgggc cataggagag cgcctcggca
     6841 ttcctgtgga ggagcggtcc gggttcacca acatggccct gggcccctgg gagggacaat
     6901 ccaaggatct catccgagag acctatccgg aggagtgggc cctctggatc cacaaccccg
     6961 agcgcctcgc cctgaaaggg gccgagaccc tggacgacgt ccagcgccgg gcctacgcca
     7021 acctggacca cctggtgcgc ctccacgaag ggaaggcctt tgtggtggta tcccaccggg
     7081 cggtgctgaa gcccctgctg gcggcatgcc tggccattcc ctccccttat ttctggcgcc
     7141 tgcacctgga cacggcgtcc tacagcgtcc tgtggcatga aaggacccga ggatacacct
     7201 gcgccctgtt gaatcagacc cgtcacctgc ccgacctggt gtcggagtgg acctagaccg
     7261 gaggaagacc atggccacgc ctgccctgga gattcgccac ctatccaagc gcttcggatc
     7321 cttcgtcgcc aacgacgata tctccctgga agttctgccg ggagaaatcc acgccgtggt
     7381 gggggaaaac ggcgcgggga aatctacgct catgaagtgc atctacggga tgcaccgccc
     7441 ctcggcgggg tctttggccc tacgggggca ggaagtggtc gtggactccc ctcgcacggc
     7501 caaccacctg ggcatcggca tggtgcacca gcacttcatg ctggtaccat ctttccccgt
     7561 ctatcgcaac gtggttttgg gggcggaacc aggccagtgg gggtacttcg acgagggggc
     7621 cgccctccgt cgagtggagg ggctcacgaa ggagttcaac ctcgccgtgg atcctcgatc
     7681 caccaccgcc accctccccg tggggctcca gcaacgggtg gagatcctca agctcctcca
     7741 tcgaggcgcg gacatcctga tcttcgacga acccaccgcc gtcctgtccc cccaggagat
     7801 cgagggtctt ttcgccattt tggagaactt ccgacgctcg gggaagacga tccttttcat
     7861 cgcccacaag ctccgggagg tgttggcggt ggccgatcgc atctcggtgc tccgccaggg
     7921 caagttggag ggcaccgtac gccgagacga agtgaacgaa gccaccctgg cccgcatgat
     7981 ggtggggcgc gacctccccg ccctggcccc cggagcccca ccgacctcgg ggaaggagct
     8041 cctccgggcg gaggggttgt gggccctggg cccccgagga gagtgggccc tgcgagatgt
     8101 gtctttttcc gtggcggcgg gagaggtctt gggaatcgcc ggggtcatgg gcaacggcca
     8161 gagcgagttg gaagagtgtc tttgcggcct gcggcatctc cgggagggta aaatatttct
     8221 gggagaccgg gacatcaccg aagctaccat ggcctcccgg cgcgaagggg ggctggccta
     8281 cgtccccgag gaccgcctcc gcaccggcat cgctcccctg gcccgcctct gggaaaatat
     8341 gctcctggga caccagcgcg aggagcggtt tctccgggga ggtcccctgg ggtttcttcg
     8401 cccgggggcc atccgccctt gggtgcgacg gaccatggag ttattttcgg tgaaggccgc
     8461 ctccgacatg gtgcaaggag gaactctttc aggaggcaac atgcaaaagc tcgtgatggc
     8521 ccgagagatg gccttcgact ctcccttact tctcgtgtcc cagcccaccc gaggggtcga
     8581 cgtggggggg atcgccttca tccacgagca aatcctgcgc caccgacgcc agggaggggg
     8641 ggccctcctg atctcctcgg acctggatga agtgctagcc ctggcggatc gggtggcggt
     8701 gatgtaccaa gggcgcatcg cggcccttct cccccggaag gaggcctctc gcgagctggt
     8761 ggggcggtac atgctgtcgg ggatggcgtg aagcgccccc ttggtccgga ccccgatgac
     8821 ccttcggggg accgggggga aaagatggcc ctttgagaac ctaaggggaa gatcttgcgt
     8881 ggtaggggcg agtaggggcg aaaggatctc gcccctactt cgtttcccgg gaatggggac
     8941 ccggactgag gcccgggggc cttacggccc cgaagatcgt tttaccgcaa aggtcgaagg
     9001 atcctggcgc tcccctacct ccgcccatcg cctcgccttc cggagcggaa cgaccgggga
     9061 gccgaaggac gggggcgatc ctcccccgaa ggaccctcgg gccggggacg acggtcctcc
     9121 cgggggcggt cgggcctagg ggaccgggga cggtcgtctc gctcccgcct aaaggtccct
     9181 tccccttccc gatggggagg acggggacgg tcgctaaagg ggcgatcccc gnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nttcccgatg gggaggacgg ggacggtcgc
     9361 taaaggggcg atccccggcg cttcggcggg ggcgatcctc ccccgaagag ccttcgggcc
     9421 gaggacgggg ctccccccgg aaacggtcgg gcctaggggc ctgagggcgg tcgtctcgct
     9481 cccgcctaaa gggcccttcc ccttcccgat ggggaggacg gggacggtcg ctaaaggggc
     9541 gatccccggc gcttcggcgg gggcgatcct cccccgaaga gccttcgggc cgaggacggg
     9601 gctccccccg gaaacggtcg ggcctagggg cctgagggcg gtcgtctcgc tcccgcctaa
     9661 agggcccttc cccttcccga tggggaggac ggggacggtc gctaaagggg cgatccccgg
     9721 cgcttcggcg ggggcgatcc tcccccgaag agccttcggg ccgaggacgg ggctcccccc
     9781 ggaaacggtc gggcctaggg gcctgagggc ggtcgtctcg ctcccgccta aagggccctt
     9841 ccccttcccg atggggagga cggggacggt cgctaaaggg gcgatccccg gcgcttcggc
     9901 gggggcgatc ctcccccgaa gagccttcgg gccgaggacg gggctccccc cggaaacggt
     9961 cgggcctagg ggcctgaggg cggtcgtctc gctcccgcct aaagggccct tccccttccc
    10021 gatggggagg acggggacgg tcgctaaagg ggcgatcccc ggcgcttcgg gagggacgat
    10081 cttccccttc ttccgacgcc gacttcagcc tgccacaggc cccttccacg tctcctcccc
    10141 gaggaactcg gagttccaca ttcacctggc gttcctgcaa aaggcgccgg aagcgttcca
    10201 tgcgatcctg gggagggcac cgaaagttcc cgtcgtcccc gttgtatgga ataaggttga
    10261 caaaagcatt aatcccccgg acgaaggccc ccagggcttc tacatgggga agggtatcgt
    10321 tcacgtcttc caggagggcg tattccagcg ttatccggtc tccggtctta gcctggtacc
    10381 tcaagagggc ttgacgaagc tcccccaggg ggtaaacccg gttcaccggc ataagctgcg
    10441 accgcaactc atcttccgcc gcatggaggg agacggccaa acgcactccg agccccgaat
    10501 cagccaaggc gtcgatgccg ggcaccaccc ccgcagtaga aagggccatg tgccggatcc
    10561 ccaggtggcg cattcccggg gcgttaagga tccgcagggc tttgaacacc tcggcggtgt
    10621 tgagcagggg ctctcccatg cccatgagca ccacgttctg gaccggggcc cctaggtggc
    10681 gctccatggc caagaagtgc cccgtgatct cccccgccgt catgtcccgg acaaagcccc
    10741 cttttccggt ggcacagaag gcgcacccca ggggacaccc cacttgggac gaaaggcacg
    10801 ccgtcttgtg ggtggcatgg ggcagaaaca ctgactccac ggtggcccca tcccccagcc
    10861 gccagaggaa tttcttcgtg ccgtcccgca gcgaagttct ctcttttacc agatccggca
    10921 gaatcagatc cacggcatcc gtcagcttct gcctgagccc ttgggatagg ttagtcatgt
    10981 ctttaaagga aaagatcttc ttgcggtaga tccaatcccc gatctgttcc gctcggtaag
    11041 ggggttcctc cagggagatc aaaagatctc gccagttttc cagtgtcagc tccagacccg
    11101 atacgcgtgc catggacgtc tcctcccgcg ccccacggcg ccatgtattc acccattgca
    11161 gagaaaaaac catcggagcg cctctgggca ctccgatggg cgaagatcct ttcggccaag
    11221 ggacattatc tcacatatcg tccttttttc ccaaggggcg tcagatcgta ggccagggct
    11281 ccgagaacgc aggatagagg ggaaaggcgc cgctgagctc tttcaccgca gctttaaccc
    11341 gggcgtgctc tgcggcatcc tcgggacggg ataggacccg atccacagcg tcggcgatgc
    11401 gccccatctc ggactccttc atgccccgag tggtcacggc ggccgtccct atccgaatgc
    11461 cgctggttat cataggcttt tcctggtcaa aggggatcat gttcttattg accgtcaccc
    11521 ccgcctcgtt gaggatgcgc tccgcatcct ttcccgtcac gccccgggag gttttgcagt
    11581 ccaacaggat gaggtggttg tccgtacctc cggagacgat ccgatagccc cgatccacca
    11641 gctcccggga tagggccttg gcgttggcca ccacctgggc cgcatagact ttgaactcgg
    11701 gctccatggc gaggcggaag cacaccgcct tgcccgccat ggcctgcacc aagggccctc
    11761 cttggatgcc ggggaagacc gtccgatcca ggtctttggc gtactgcgcc gtgcagaggg
    11821 ccagggcacc ccgggctccc ctcagggtct tgtgcgtggt ggtggtgacg aaatctgcgt
    11881 ggggaacggg gctcgggtgg gccccccctg ccaccaggcc agcgatgtgg gccatgtcca
    11941 ccaaaaagac cgcccccacc tcccgggcaa cgcgggcaaa acggccgaag tcgatatccc
    12001 ggggataggc gcttcccccc gccacgatga cctgggggcg atgctcccgg gcaaggcgtt
    12061 ccatggcgtc gtagtctatg gtctcggttt cccgggtcac cccatacccc accacgtggt
    12121 acatcttgcc cgtgaagttc agcgggtgcc catgggtgag atgccctccc tggtccaggc
    12181 tcatggccaa aagggtgtct cctggcttca tcacagcgaa gtacaccgcc atattcgccc
    12241 cggaaccgca gtggggctgg acgttggcgt gatccgcccc gaagagctcc ttggcgcggt
    12301 tgatggcgat ctcctcaatg gcctccacgt actcgcaccc tccgtagtac ttcttgtagg
    12361 ggtacccttc ggcatacttg ttggtgagga tcgacccctg ggcctccatc accgcccggg
    12421 ggacgaagtt ctccgaggcg atgagctcca ccccatctcg ctgccgcgag agctccgact
    12481 tcgtcaggcc gtaaatttcc ggatccagat ccgccatgcc catttccatg atctgtcgca
    12541 tctctatcat catctcctta atactatttt tatctttagg aggggaagga gccccttatg
    12601 gtctcatccg atgcgaagtc ccccgggccc cggggcgcac aaaaagggcc tggctctccc
    12661 accaggccgc cctcgacgag acagctcaat ccttacggcg cccccaatat gacacaacaa
    12721 aaaagagatg tcaatcctcc ccgaccggcg tcgtcgcggt taaatatcag taatagctaa
    12781 attgcgaagg tcgtttttaa ggcggcgcca ccgaaaaccc cccagcgcaa aggcggccta
    12841 tctccgccgc gccagaaaag aaaaccgcaa cgccgcctgg gctccctcag gcccggcgcg
    12901 gggctactct ccttcttcct cctccaaggg agcaagctcc gtggcccctg cgggcatggg
    12961 aagggcaaag gggtcatccc cttcttccac ccggaagcga ttcaccaggg aatcgagctc
    13021 gttggccatg ctgctgctct cttccgaggc ggcggagacg ttttccatca ccgacaccac
    13081 gttccccgtg gccaccaaaa actcctgggc atggcgctcc gcctgctcaa tgaccacggc
    13141 ggtgcgctgg atgagctcca cgatgcggtc agactgagac acttcttcgc tcgtggtctt
    13201 ttggatctta ccgatatcgt ccacggagtg ctgggccgca tccacgatgt tcgccagggc
    13261 ctcgccggac tgacgcaccg cccccacccc ctcttcgatg cgggcgcgac tctcctgcac
    13321 ggacagaacg gcggtctgcg tgccctcggc gatcttggta accagctccg ccacttcccg
    13381 ggctccccgc tgggactgtt ccgccagctt tcgcacctct tccgccacca cggcaaagcc
    13441 cttccccgcc tctccggcgc gggcggcttc aatggcggca ttcagggcca ggaggtttgt
    13501 ctgattcgcc agccccgtga tggtgtcgct gatgatgccg atgcgggcgg aaaaggccga
    13561 aagtcccccg atgactttct ctgtctctag ctccttttct cggatcgtct ccatgtgatg
    13621 cacggcggta tccaccgtgg cgcgtccgct cgacgcggtg gccagagtcc tttcggcctg
    13681 ctccgtggcg gagcgggcca ggttggccga tatctggagc agcgacgaga gctcaagcaa
    13741 cacctcgctg gtttcctgca ccgccgcgaa ccccatggtg gtggccccgg aaagttcggt
    13801 gtttttgtgg ctcagatcca tcgtggtggc cgtcacttcc tgtaccgacg cggcggtctc
    13861 ctcggaggcg gcggcgagct cccgggcggc ggtcttcacc cggaggatca cctctcttag
    13921 ctctttcacc atggtgtcga aggcgtgggc caactgtccc acctcgtcgc cactctggta
    13981 atcgacggaa acggtgaaat ctccatcgcc gatggcccga cagtgctcca cgacgaatct
    14041 tagaggcttg gtgatgcttc tcacgatgcc cagcgccacc agggccccga gcagggccgc
    14101 gatcaccatg ccgtaggaga gaacccggga ggacagggac agggcctccg aggcccgctg
    14161 caccacgccc ttaccttggg tgaagccctc aagagaaagg gcattggtca ccgccaacac
    14221 gcccttggag gcagtctcca gcgattggga cagggccgcc agcttggtat agccctccac
    14281 caccaccttg gcggcggcga cgtaatctgc ggtggcctgg cgcaccgcct ccatccgctc
    14341 cttgtccttg ggccggatgc tgatcatgat gagttcgtcc acgtaatcgt tcaccggggc
    14401 aaaccccccc agggccttct ctatgacggc ggggtcgcgc gccagctggg ccttgagggt
    14461 ggccacgtta gcctcttccc ccgtggagag gatgcgggac actatttcga tcttggagag
    14521 gcgatctttg acgaaggaag ggtcttctcg gtcggagatt tccttgtcca gttccttgtt
    14581 ttccagggct agaaactcgc tgcagatggt ccggtaggag agaaaggcct tgtcttgttt
    14641 ctcccgcgcc tctcctatgg ctttgttcgt cttctgggct tccttcgcct gggcctggta
    14701 ctccgccacc ttgcccccca ggtcatccag gctctttagg aactccgcca tcttttctcg
    14761 gtccgccagc tcccgggctt cccccaaaac gcgctccagc tctcccaaga tctggatcac
    14821 gccgttaaaa tccccctgct tcatggtcag gttgtaggtc tggaagcacc gcagggcctg
    14881 gaggagataa cgctccagcg tgctcgaaag ctccacctgg ggcatggccc ggctcgccaa
    14941 aacgccgatc tccccccgga tgagccccgt ctggtaaact cccaaccccc ccatgcagac
    15001 cacgatgata agcacgatgc caaagcccaa catgagccgc acaccgatct tgaggttttt
    15061 aagcatgcct cgccctcctt cgctctttag gccacgaacc gccccaaaac ctaagccccc
    15121 gcaataaaaa ccaccccaga ggtccttatc tcaccgcaga cgcatctaaa agccttctac
    15181 tggatcgcct tttcagaaaa gaacggactt ccttcctccg gcctccgggg ctccatactc
    15241 ctccaggata gcacactccg gcggcataaa aaagcactcc ggggcgtctc cttttgtcaa
    15301 aagaactacc ccaggacctt ttccaaaaag cgccataggt tccctccgag gaccgcgttt
    15361 atctctcctt cgtcgtaccc ccggcgacga agggcctggg tgaaacgccc caggtcgccg
    15421 tgtccttcca tgacatcccg ggtttggatg ctccccccgt cggagagggc gtcgcgaatt
    15481 tggtcacaga aatcgaaccc cacccccacc gccgcgatgc ccgccacttt tttcacgtga
    15541 tccacgtgat cccccagcac gtccaccaag tcgcgggatt cttcccgggg cgccgaagag
    15601 acgaaggcgt cacaggcatt catccccgtg agccctcccc gggcccccag ggtgcgcaac
    15661 tggtcatccg tcaggtttcg ggccacgggc accagggccc ggcagttgga gtgcgacgct
    15721 ataaagggtt ttcgggaaaa tcgggccacg tcatcgaacc ccgcgtcgtt cagatggctc
    15781 acgtccacga acatccccat ctcctccacc gccgccacca agtccacgcc gaagggagta
    15841 agccccccgg ggcgtccttc gttctcgggg aagaagtgac atccgtcccc cacctcgttg
    15901 cggcggctcc agaccagccc cactcctcgc actcccagat cgaaaaaaat cctcaggaga
    15961 gccatgttcc cctggagggg ttccgccccc tcgaaggaaa taaaaactcc cacggcgcca
    16021 cgcctccggg cggcccggag gtcttccatg gtccggcata ggaccacggc gccgtcgcac
    16081 tccttcaggt cggcccccag ggcgctgatc tgctccaggg ccttgcgcaa ggccatctcg
    16141 gggaggaaaa cgttggggat gaacagggag gagatcaccg cccccacccc cccccgccgg
    16201 aaggccggaa ggtaacgctc cgccaccact cgccgctctc cccggcgtcg ccggagctcc
    16261 acgtcatacg ctaggtcgag atgggcgtcc atcacaaagg ggatttcccc cgttcccgga
    16321 ttagtcacgg ccgtccccct aggcccggtg ggccaccacg ccgtccacca ccgtggccac
    16381 cgccacggag cggggagaaa agggcgattc ggaccaaagc accgcgtcgc cccccttacc
    16441 ctcctccagc gacccgtacc gcgcctccag gcccagatgg gccgccgcag tccgggtgac
    16501 cagggccagg gcctcttcct cggaaaggcc ttctcggatc gtttcggcgg cttcgttcct
    16561 gaggtaaccg atgggaatca ccggatggtc cgtcatgagc gccacgggga cccccgcccg
    16621 cactagggtt accacgtttc ttatgtgagc ctcccggagc tccaacttgg acctgaaggt
    16681 catggcgggg ccgaaggccg tccgaacccc cttggcggcc aagaggctag agatcaaaaa
    16741 gccctcggtg ccgtgctcca aggtgtaatc caggccaaac tcttcggcga tacgaaggcc
    16801 cgttaggatg tcgtccgccc ggtgggcgtg catcctcagg ggcatttccc gccggaggag
    16861 ggcccccagg gcctcgtccc ggaggtttct ttcccggggt ccctttttcc cccggacctt
    16921 ggcggcgtag ttttccgccc ccaccagggc atcgcgcagg caagccgcca cgcccatgcg
    16981 cgtggagggc cagagatttt tacccttgta gacgtttttg ggattctcgc cgaaggcggc
    17041 cttcatgccc gagggggcct gccgcaccat gtcttccacg gtgtctcccc aggtgcgcac
    17101 cacggccccc tgccctccca ccacattggc gctccccggt aggatctgca ccatggtgac
    17161 ccccccggag cgagcctcgg cgaaggcccg atcccggggg ttaatggcat ccaccacccg
    17221 caggtgaggg gtgacggggt tcgtggcttc gttgccactc ccctccgcct cgggagcccc
    17281 ttcttcccaa aggctcacgt gggtgtggac atcgatcaac cccggggtga ccaccatgcc
    17341 ccggccatcc agctcctccc catcgccccc tcttagatcc ggccccagag aggctatgat
    17401 cccgtctttg atcagcacat cggtcctatc caggatcccc cgaggaccga cggtccagac
    17461 cgtcgccccc cgtatcagca aattgccccg ctctcgcact ctcaaccatc cctttcttcc
    17521 cggtgatagg gtactcccag agagcgggga gcccccagcg acacggcata tcccttgcgg
    17581 accgcgatga caaaaaggac cgcgatggtc atcacatagg gcaacatcat gaggaggggc
    17641 gcgggcaccg tggtgccccc ggcctgcatg cgaagctgca atgcccccac acccccgaag
    17701 aagtaggctc ccagggcgct acgcaccggg tgccataggc cgaagatcac cagggccagg
    17761 gcgatccatc ccctcccggc ggtcatcatc tccgcccaca tcttagtata gaccacggac
    17821 atgtacgccc ccccgagccc caccaggccc ccccccaaga tggtgtaaag gtaacggagg
    17881 cgcaccgccg acagccccgc cgcatccacc acccgaggag cctctcctgc ggccctgagg
    17941 ttcagccccc cccgggtgaa atacaaaaac cacgccagga aggcggtgag aataaaaccc
    18001 ccgtagacca gcacgtcgtg ggaaaagagg atgggcccca cccagggcaa ctccgccaat
    18061 cccggcaggg gagccgccgg aaggccggga atggtgactc ccaccaggtt tttccccagc
    18121 aaagcactgg ttcccgtgcc gaacatggcc agggccaggc cgctcaccac ctggtttgcc
    18181 cctaaggaaa cgcaaagtac ggcgtgtagc gccgaaaggg ccatccccac caataccgcc
    18241 aaggcgatcc ccgcccaggg gttggaaaag acgagcgctc cccagaaccc cgagagggac
    18301 cccatgagca tgatcccttc caatcccagg ttcatgaccc ccacccgttc cgtaaggagc
    18361 tcccccaggg tggcaaagag gatcggcgtg ccgcttcgca ccgccgccgc catgatgggt
    18421 atcaagtact ccacgagatc actccctcca tgcggcgatc gccgccaaaa ccttcccttt
    18481 tagggggcct cggtcgagca agggccccga aatcaacatt ttttgtcccg aaataggcga
    18541 atccgatggg tgcgccacac gtctccccct aggagaaaga tgaggatgca tccctggatg
    18601 atgtgggtgc tcgccagggg caggcgcagg accacctgca gcgtgtctcc tcccaccagc
    18661 agggccccca taaaaaaggc cacgcccagg atggcccccg ggttcagccg ggccaaccac
    18721 gccacgatga tggccgtgta gccatatccc ggggagaagc cgggctgcat gcgcccttga
    18781 agtccggcga tctctcccat gcctgccagc cccgcgaggg ctccggaaaa aaacatgagg
    18841 agcaccacgt tcctcacgta ggggatcccc gcatagtggg ccacctgcgg gttttccccc
    18901 agcactcgca cctcgaagcc ccaccgggtc catcggaaaa agaaatagag ggctccggcc
    18961 aggatgagag caaaaaagat ccccagatgg actcgggtgt cgagaaaaag gggcaatcga
    19021 gccgccggag ggaagggagc ggtcatggga aagcccaggc tggcagggtc gcgccagggg
    19081 ccgtagatga agtaatccac cgtgtggaga gcgatgtagt tcatcatgag ggtggagatg
    19141 atctcgttca ctcgccatcg ccctttgagc catcccgcca gggctcccca gagccccccg
    19201 gccagggccg ccgagagcat catgaggaag agcatgacaa aaaaattatc tacaaaggcg
    19261 tagcgcaccg tcgccgccgt ggccagggcc cccaggagga tctgcccttc ggcaccaatg
    19321 ttccacacct gcatgcgaaa ggccagggac accgccagtc ccgccatggc cagggggatg
    19381 gccttcacca ccgtttcgct gaggccatac caatttccta gggcccctcg aaacatggtg
    19441 acgtaggcct cccaggggga tactcccagc gccaggagca gccctccccc cgcccccagg
    19501 gcgaggatca cggaaagcac ggacagggtt ccgccgatcc accaggggat atcttttctt
    19561 ttctccagcc atagcctagg catgaccttc ccccctctcc gcccccaggg gcgtgcccgc
    19621 catcatgagc cccacgcgct cctctcccag atcccggggg ttatcggaga cccccatgag
    19681 gcgcccctgg tggatcaccg ccacccggtc gctcagggca aagatctctt ctaggtcctc
    19741 cgaagccaaa aggaccgccg ctccccggtt tcgggcctcc aggatctggt cccgcacgta
    19801 ctgggtggcc cccacatcga gcccccaggt gggctcctgt accaccaata tggaggggag
    19861 caggcccagc tcccgggcca gcatcagttt ttgcagattt ccccccgaca gggtccgcac
    19921 cgccacgtcg caggaaaggg tctgaacccg aaagcgtcgc accagctctt cggtgaaggc
    19981 ccccaagacg tcccagcgca ggatctcttt ccaccgggaa aattcctcgc gccagtagtg
    20041 ccgcaacgcc acgttctctc gtaagttcat gttccccacg aggcccatcc ccatgcgatc
    20101 ggcggggacg taactcaccc cctggcgctg gaagatccgg gggggagccc ccgtgaccct
    20161 tcgcccccgg agggtcaatt cccccgcctc cagagggcgg agtcccacca gggcttcgca
    20221 gagctccctc tggccgttgc ccgccacccc cgccaatccc aggatctccc cccgacgcaa
    20281 ctccaacgat atcccccgga ctgcaggaag gtggcgatcg ctttgcacca ccaggccccg
    20341 cccttccagg agggtctcct ctccgccccg gggagacgga ttctcggaac ggggtacttg
    20401 gtaccccacc atctgctccg ccagggcggc cttggagatc tctcccgccc gccaggtgcc
    20461 gacgactcgg cctttgcgga gaatcgtcac ccgggaggag agggccatga cttcgtccag
    20521 cttgtgcgat atgaagacca cgccgtgtcc ttcctccgcc atcctccgga gggtggaaaa
    20581 gagggcccgg gcctcttggg gcgtcagcac cgccgtgggt tcgtccagga tgagggtccg
    20641 ggccccccgg tagagcgccc ggaggatggc cacccgttgc tgttctccca ccgaaagctg
    20701 ccaaattctc gcctccggat ccaccgccag gccgtattgg gtcgatagct cttcgatgcg
    20761 acggcagacc ttcttccggg ggatgacgga ggggatggcc cgatccccca ggatcatatt
    20821 ctcccatacc gtcaggacgg gcaccaggag aaagtgctga tggaccatgc cgatgcccac
    20881 ggcgatggca tccgaagggg accgaaagaa gtgctctttt ccgtcgacaa agatgcaccc
    20941 cgcctcgggg gtgtagagcc cgcagagcac gttcatgaga gtgctcttgc ccgccccatt
    21001 ctcccccaac agggtgtgaa tctccccggc cctcaggtcg aaggccacat cgtcgttcgc
    21061 ccgaaccccc aggaaggatt tgactatgcc ccgcatgctc accagggtcc gctccgcctc
    21121 cgtcatggca ctcctccgct ctttccccgg catggcgtac accgcgccag agtaccacat
    21181 ctcggcggct ttcgcacgga gtcctgcgga aacctcaacc gcagtccccc cggaccagcg
    21241 gaccaggggg gcttcgagag cgccccgagg ccaccgatca aaaagcccag gggggaagcc
    21301 tctttttccg aggctccacc ctgggctcag gctccggcga ccgtcgcgcc gccgccccgc
    21361 cgggaaaaga tctctcccgg ggacggtctc gccttcggcg cttcccccct acttcggcac
    21421 ctcgccctcc acgccctcca cgaaccaatc gaaggcgagc atctccgcat ccgtcatggt
    21481 ggttccctcg gggacccgca cggtgccgct ctgatccttc acggggcccg tgaagacgtc
    21541 ccaggtaccc ccgacgatct tggccttctc ttcttccacc aaggccttta cctcttcggg
    21601 gacatccttg ccgtaggggg cgaggcccac cataccgtcc ttgagccccg gccactggga
    21661 agtgctcttc caggtgccgg cgatcacttc ctgggccgcc gccgtgtaga tcatgcccca
    21721 gttccagatg ggggccgtga ggtgcttcgt gggggcatac tggctcatgt cgttgttgta
    21781 ccccaccacg tagacccccg actcctccgc cgcctgggga gccgctccgg tgtcggcgtg
    21841 catggcgatg acgtctgccc cggcgtccac caaggccttg gtcgcttcct tttccttgcc
    21901 cggatcgaac caggagaaga gccataccac ccgtaccgtg gcgtcgggat tagcgcggcg
    21961 caccccccgg gtgaaggcgt tgatgccccg caccacttcg gggatgggat gggccgccac
    22021 aaagccgatg acgttctttt tggtcatctt tcccgccacc aggccagaaa ggtaccgggc
    22081 ctggtacatg cgcccgaagt acacccccac gttgggagcg gtcttgtacc ccgagcagtg
    22141 catgaagacc acgtcgggga atttcttggc cacgtccatg acgaagtcca tgtacccaaa
    22201 ggacgtggcg aagatcagct tagagccgtt ccgggcaaag gtctccagca cccgggcgga
    22261 atcggggccc tcgggcacgg attccacgat gctgctttta acgccgggaa cggcctgctc
    22321 catggccagg cgcccctgtt cgtgcatgta ggtccatccc ccgtctccca cggggccgat
    22381 gtacacgaac cccggcttga gctcctccgg agccacgggg tcgaaggccc aggcccccgt
    22441 cgccaggatc cccaccaaca accatgcccc ccaccacaca tcgcgctttc tcatcttttc
    22501 atccctcctc ggttcttttt ggccccccat cgccccccca gggctcgaaa aggacgcagg
    22561 ggccctagcc ctttcgagct agccttcgcc ttcctctctc ccctcgggag agggctcgga
    22621 aaatcagcgg gacgctccgc ttccacccct tcccggggcg ccgagagatc gcttcgcgtc
    22681 catcccctct cggaggtgga cgcaatctcc ccgggcctgg aaacctgggc aagagcaggt
    22741 cccttctgcc aggttcacca cgtactcgtc gggggctccc atcaccaatg cgatatggtc
    22801 cgagaccaat ctcagggtcc catggcgcca ctcttctttt tcttccggca attcacccct
    22861 gtcggggcga ggaggagcgc tacggcccct ttcttcctcg cgatccaaga cctcgttcac
    22921 caaatcatcg gcgtggtcgt tatccttccg ggggatccat tcgaaacgaa cgtctccgcg
    22981 caccaggctt cgggctcgtt ccgccaaggc ccgcaatcgg ggctctcgaa tcttccattc
    23041 ccctcgcatc tgggagatca ccaggcggct atcccccaac accacgccct tgcaaaaacc
    23101 ccggcggtcc acttcctcca ggagcatgat caacgcctcg tactccgctt cgttattggt
    23161 ccgtcgcccc aggaaacgcc cctcttcgaa gaccacatcc ccccggtcgt ccaagagcac
    23221 cgcccccgcc gccgcaggcc ccggattccc ccgggaacct ccgtcgaaga aacctaccca
    23281 gtttttcatc gcccatccca tcatgttccc cttcgtttgg cctcgtacat ccgttcgtcc
    23341 gcccggcgca aggcctccac gatggacacc ccgtcccgag ggcaggaagc gatgccaaag
    23401 ttcgcctggg tcctaagctc cgggacggaa aaggccaact ccttcacgcc ctgccccagc
    23461 tcctccacca gcctctctgc ctcctcaagg gttttatctg gaaggacgat gagaaattcg
    23521 tccccgccat agcgccccgc gaagcccact ccttccaatc gcttgtaaaa aaaggccgcc
    23581 acagtggtaa gcacctcgtc tcccacggcg tggccatagg tgtcgtttat caatttaaag
    23641 cgcctcaggt ccaccatgac cagggtaagg gtgccccctc gtcgcaccag gtcttctcca
    23701 tcgccctcaa ggtaggagaa caaaaagcga cggttccata tccccgtgag gccatctcgc
    23761 atagccttga gctccgtcat gtccaagagg tcgttcaccg cccaaagggc ccccaggtgg
    23821 cgggccagga tgacgaagag ctcctcgtcg tcctgggtga aggtccgggg cctggggctc
    23881 tcgaagcaca gcaccccccg cacccgctct ccgtgcagga tggggatatt cagctccgac
    23941 cgggtatcgc tgtgtcctcg gacgtagccc gaggcctgat ccgtgtcggg aacggacacc
    24001 accttacggg aagtcgccgc ctgggccacc gtggactgga aaagccgacc cgggcggagc
    24061 accacgtccc tccagagccc ctcggcctct tccatgctcc cggaggaggc catggggaag
    24121 atctccatcc ttcccccttc ttctctcacg tcgaagaccg tcaccagcat gtcatcaaag
    24181 gcctctcgca gcagggcgat gatccgcagg gctatggagc ggttgtcccg ctccccggag
    24241 agctcgtgca ccatgccgtg gaggaatctc atgagcttga tctcccgggc aagcatgtcc
    24301 aggttggcga tgttttccag caccacggac ataaaccccg ccaccaaggt gagggtttcg
    24361 aggtcccgga gggtgtaggc gccggggcgc cgggcgatgg ccaccacgag gccccagagg
    24421 aagtcccccg accggatggg caccaccagc tcggaccggg tgccgtagtc atcggggtca
    24481 aaataggggt cctgggcggt atcgctacag aggtagggct ctaaggtctt caccacgtgc
    24541 cccacgatgc cagagccccc catgaggagg ctttcttgct ccagatcaag ctcaatgccc
    24601 agggaggcct ggcaagacag gggagcctcg cggtgatctc ggccgaagac gaggaccgtg
    24661 ggaaaaccga gctccctcac cagaatttcc atgatgagga ggtagagctg ccgggcgtcc
    24721 cggcagacga agagcgtgcg ggaaacgtcg atgagggcgc gctggatccg ggcgtgctcc
    24781 tccaccgccc ccttctgggc gttggcttct tccagggcgc ggttcagggc ctcctgggcc
    24841 ttgatgaggt tggcccgaaa catcttccct cgctcaaggt tctttcgaaa ctgcgtttcc
    24901 gtctccacgt gggagaggac gctccccagg atccccgcca agagtcgagc aaagcgcacg
    24961 tcctcatcgg agaagcagtc ccgctgggac gattccacgt ccaggacgcc gaaaaccgag
    25021 ccgtgccaga ctatgggcac ggagagctcc gcctgggtat ccaacatgtc ctggaagtag
    25081 tccggatctt tggtcacgtc gttgaccact tgaatctctt ccgaagcaaa ggagcgccag
    25141 cacaccccct tgccccgggt aatccgttcc gccgtggggt tatggttggc gtaggccgtg
    25201 agttccaggc tgtccgtccg agggttgtag aggaatatgg ccgggcgaga gtaacgggtc
    25261 tgctccgcta tgatggtcgt ggccagggca caggcccgct gggggctttt agcccggtcc
    25321 acttcgttga gatagcgaag gatgatttcc atggccgacc cttcggtgag ccgaaaggat
    25381 ccttcttggg ccgaaacgat tgatgcggga ccttccacct tctccaccgc cccgtcctta
    25441 caccgtagat ccgactagga aaaccacttc cggaaaagct cctcgcggca ttggcggacg
    25501 acttgatcca ccaacgcctt gaactcgggg tccacgtcct tgggcatcgc cactccgtag
    25561 cgctcccggg aaaggagaac ccccaggacg tggagctcgg ggttttcccg ggccttctgg
    25621 atggccaggg gactatccac caccacggca tccaccgtcc cttccatgaa ggccgaaaaa
    25681 gcgtcgctcc acgtggcaaa gggcacggcc tcgctccgag gcatggagtc tcgcaccacc
    25741 gcctcactgg tggtaccccc ctgaaacgac acccgacgcc cggccaggtc ccccgtcgtc
    25801 cgggccccca gggcctttcg aaagagcacc gtctgtccgg cggaaaagta ccaatcggag
    25861 aaggtcacct tctgggcgcg ctccgcatcc gtcgtgatcc ccgccaagag aaggtccgcc
    25921 tccacccagg ttcccgactc ccagcttccg gaaatggtgc ccgcctctcc ctttccccag
    25981 gggatgggca tcacccggag ggaaaccccc accgcctggg cgatgcgctc cgccagatct
    26041 acctccagtc cctccaccga tcccttatcc catatgtgaa agcgtcccca ggtgtcgtct
    26101 tcgatgccca ccaccaactc tccccgggcc cggatctcct gaagggaccg aggggaggcc
    26161 ccttcggcca cagaagagcc caccgcaaag acggccataa cccataacac ccatcccatt
    26221 ccacgggacc aacggttcat ctcggccacc tcccgagcac tcgactccga accatcatag
    26281 cacgtccccc cctttccgcc cacgcctttt ttgatctttc acccctttgt ggtaaattgg
    26341 gagcgctcca ggaactatct ccctggtctt ttaaggaggc tcccatgacg tggtatgacc
    26401 tgacccatct catcacttcc gccatgccgg tataccccgg caccgagccc ccagagctcc
    26461 tggaggccac caccctggag agggagggct ttcgggagac gcggctccgc cttttttccc
    26521 acacgggaac ccatatggac gccccctccc acatgatcgc cggggctcca tccctggatt
    26581 ccatggccat cggcgccttc gcgggagccg ccgtcaaggt ggacctctct tcctttcctc
    26641 cgggaagggc ggtcccccgg tctttccttg aggaggccct ggagggggcg tttaccccgg
    26701 attttgtgct cctccatacg ggatgggatc gcttctgggg caccgaggag tacttttctc
    26761 ccttccccac cctgaccccc gacggggcgg cgtacctggc cacccgggcc cttaagggcg
    26821 tgggggtgga tgccatctcc gtggatccca tggaaagcgt ggccttcccc gtccaccgcg
    26881 ccctcctttc ccggaggctg gtgatcgtgg aaaacctgcg gggtctgggg gagctccccc
    26941 cccagggatt caccttcttg gccctccccc tgcactacgc cgacgccgac ggcgccccgg
    27001 tgcgagcggc ggccctcttg tccgcctagc cccgaccttt cggagctcag cgcccccgga
    27061 ggccaaatga catcgctatc gctcatattg gggggactgg ggctctttct cctggggctg
    27121 gacgacagca ccaggacctt ccgagaggag tggggtcccc ggtcccgccg cctcttcgga
    27181 cgcatgtccc gctccaccgg ggcctctttt ttgctgggga cggtgctgtc ggccctgtcc
    27241 caaagtagca ccgccgccac cgctttgacc ataggattgg tcaacgccgg agttctctcc
    27301 ctcgctggcg ccgtggtggt catgatgggg gccagcgtgg gcccctcctt gatgaccttc
    27361 ttgatctccg tagacgtgac ctcctacgcc cccctgatcc tcggggtcgc cgtcttcgga
    27421 gagcgcttca cccgaggacc ttttcgccag ctctccgccg tcgtccgggg cctggccctc
    27481 atcctcacgg gcatggccct ggtgaaactc ggcgtcgtac ccctcaccgc ccgggccgat
    27541 ctccaggagc tcttcgcggc ggcggggagc tctccatggc tcctgggcct gggggtgctc
    27601 gtcgtcaccg ccgggaccca gagcagctcc ctggtggtgg ctctggccat ggtccttttg
    27661 tcccagggac tccttccccc tgagggggcc ctccccgtgg tgctgggaag ccacgtgggc
    27721 tcttcggcca ccgttctcct ggccgggctc ggaggacgcc cccaggcccg agctctggcc
    27781 tgggcctcgt tgatctacaa gatcgtgggg gtgggagcgg cgctgctttt ggcctcccca
    27841 attcagggag ttttcgcctc cctgacgttc cctttgcccc tgcgctttgc cctgctccag
    27901 gggatcatcc tctggggcaa cgcggccctt ctcctccccc tggctccctt gctggcccag
    27961 gcaagccaag gggtcgcccg gctcttcgca cgggaaactc cggggactcc cctttatctg
    28021 gacgatgcgg ccctcccctt cccctctctg gccctcgccc tgctttcccg ggaaatggtg
    28081 agactggcca gcgccttaga acagctcctc acgttgaccc tggcggatcg atctcgcccc
    28141 cgggatcgat ccgcattgca ggagggcctt ccctccttgg ccgaggcctg cgcggacttc
    28201 ctcctttcca ttccctccgg cgccgacagg ggcgtggagg cctcccggga agaactcttc
    28261 gacagcctac gatcccttag ccagctcacg acccaactcg tcacccgctt cgccccccgg
    28321 agcgacgccc tgggatatag gctcacggac gaaggccccg acggggttcc ctggaaggac
    28381 tttgcctccg ccttggtgga gctggtgcgc cacagcctgg gcacctttgt gctgggagaa
    28441 agggagcctt cccctctggc aaggcggtcg tttcagaatt tccacgtcca ggaaaacctc
    28501 ttgagggctc gcctcagggg ctccggagaa gggcgggtaa cccgagagac catggaggcc
    28561 ttggaatggg tcgacgaagc ctcttccctc gcccgtagcg cccaagaact ggtgtcctaa
    28621 aaggactact cttcggagag aaggtgaatg ggatgttttt ccaccgcaac tcctccacgg
    28681 aacgccttga gaattactta tacgaccggg accgcaagcg catcctcctg atggtgtccg
    28741 ccatgggaga gcggaccgcc cgcgccctgg aggaagcggt ggaggccctc gtgacactgg
    28801 atggcccccg ggccgccgcc gtcgtcgcgc aagacggcgc catcgatgcc ctcgaggggg
    28861 aaatcgaaca ggaatgcctt ctatccctcg ccatgcgcca gcccgtaaga gaggatctgc
    28921 gcttcgtctt ctccgtgctc aagatcgtca ccgacctgga gcgcctgggg gacgaggcgg
    28981 ccaacatttc ggaaaaaacc atggcgctcc taggggcccc cttgtttcag cccctggtgg
    29041 atatccccca gatgggaaac ctcgtcgccg ccatgcttcg ggacagcctc caggcctttg
    29101 cccaaaacga cgcccccgga gccctggagg tgatacgccg ggacgatgtg gtagacaccc
    29161 tctacgacaa ggtctttcgc gacgtcatgg cccagctcat cgccgacggc atcgccgaag
    29221 aagaccgcgt gcgccggggg gcggccctca tcctgatctc ccgatacttg gagcgcgccg
    29281 gggaccacgc ggtgaacatc gccacccgag cctatttcat gatcaccggc aagaggctcc
    29341 ccgaccgaca cgaggcccgc cggagccccg ccccagagaa agggcagccg taacaaaagg
    29401 gctccccgaa gttttttcgg ggagcccttc tttcgtgtcg gagaggccct ttgggcctcg
    29461 aaaaaggtct ctagatgacg ccctgtccca ccatggcgtt ggccactttg atgaagcccg
    29521 cgatgttggc gcccaccacg tagttcccct ccacgccata gtccttcgcc gtgtcgtagg
    29581 cgttgcggtg gatggtcttc atgatctggt gcagccgggc atccacttcg ctctcggtcc
    29641 actggagccg gaggctgttc tgggacattt caaggccgga ggtggccacg ccccccgcgt
    29701 tcgctgcctt gccgggggcg aagggaacct tgttacccac gaaggtctcc atggcctcgg
    29761 gggtgcagcc catgttggaa acttccacca cgtacttcac gccgttggag agcaaggcct
    29821 tggcatcgtc gccgctcatc tcgttctgga aggcacaggg catggcgata tcgcacttca
    29881 cttcccaagg acgcttgccc gggacaaact gggctccggg gaacttgtcc gcgtagggct
    29941 tcacgatgtc ctggttggag gagcgaaggg agaggaggaa ttcgaccttc tcgccggaaa
    30001 tcccgtcctt gtcgtagatg tacccgtcgg ggcccgcgat ggtgacgacc ttggcgccca
    30061 gctcggaggc cttgagggcc gtcccccagg tcacgttgcc gaagcccgaa agggcgatgg
    30121 tcttgccctc gatggagtcg cccttcttat cgagcatctc ctggaggaag tacaccgcgc
    30181 cgaagcccgt ggcttcggga cgaatcaagc tgccgcccca gttgacgttc ttaccggtga
    30241 gcacgccacc ctggaagtcc cgggtgatct tcttgtacgt gccgaggaga tagccgatct
    30301 cccgcccccc cacgccgatg tccccggcgg gcacatcggt gtccggaccg atgtggcgga
    30361 agagctcccg catgtaagac tgacagaagt tcatcatttc ttcgctgctg cgccccttgg
    30421 gattgaagtc gctgcctccc ttgccgcccc ccatgggaag gcccgtgagg gcgttcttga
    30481 aggtctgctc aaagccaagg aacttaagga tggagaggtt gacggaaggg tggaagcgca
    30541 agcctccctt gtaggggcca agggcgctgt tgtactggac ccggaaaccc tggttcacct
    30601 ggtactcgcc cttgtcgtcg cgccagggaa cgctgaagat gatgacgcgc tccggaatga
    30661 tcatccggtc cacaatgcgg tacttctcgt actcgggatg ctgagccacc accggcttga
    30721 cggactccag gacctccacc gctgcctgga tgaactcggt ctcccagggc cacttggcct
    30781 tcagttccgc gatcaacccg tcgatatagt gctcgttagc catttccctt tgcctccttc
    30841 atgattttgt ggccgtcgcc acccataccc cggggggcgt ccccccgcct tcgaaccttc
    30901 gcgtaaatta tagcacgaag caaaaaaaat gcaccatgca aagtacgccg cagtcttttt
    30961 tctcaccccc cacccccggc ccccagggaa agaagcacca gggtggcgtc gtctctgggg
    31021 acccagcgcc cctcactcac ctccttctgg accagggcgg ggacccccga ggggtcgcct
    31081 tctctcagga ccccctggag ccacgccgcc aaaaggctcc ggggagattg ggattcgtcg
    31141 aggggcaggg cctccagaag gccatcggaa caaaagcaca ccacgtcccc cggagatccc
    31201 tccacttcgc gcccttcggg ttccacgggg agcacgccga gcaagggcat ggtggagtcc
    31261 agcgccacca tctcccccga agagcggagg ataaaagccg gagccccggc ggcgctgagg
    31321 taagaaaaaa ccttctttcg ggggtcccac acgccgtgga taaccgtgca gaagggggcg
    31381 tcatcggagt cccccgccac gcttttcagc ccctcgtcca acagggaggc cagctccttg
    31441 ggggacaggc tctcccggag gaggcggctg aaaaaaagac cccgcaggaa ggtcacgagg
    31501 agccccgccg ccaccccgtg ccccatggcg tccgccagaa ggagatgaac atttcccgcc
    31561 gaatcccgca gcaggtcgca ggcatccccc cccacctctc cccaggggcg ggtccatatc
    31621 gccgccactc cctcgggcca tcgaaggacc tccggggggc gcatgctcct ctgggccgcc
    31681 gccgccaagg caagctcttt tttaaggcgg gccgcctctt cctcccggcg ccgggcgtga
    31741 tccatggatt ccagccgaag gcccaagtga agggcaagga ggcccgccag gcgccgttca
    31801 ttgtcgcgaa agacccccga ggggagaggg ctccccccca ctcctcccca gaggacccct
    31861 ccctgggtca ccggcaccgc caagaagctc tccgccccgc cgggggccag acccggtatc
    31921 tgggaagcga gaacggaagc gggcccttcc agggaacgca aagcttcctg gtctttcggt
    31981 gaaagggaca tcccctcccg gagattggct ccggcgcccc gccacctcca gacgtcggag
    32041 gagggagaat cccgcatcac caggagagcg ggccccaggg gcaagaggga acgcagacga
    32101 tgttccagca ggtccaacac cgccgatacc gaaggacact tctcgagctc ccggactaaa
    32161 ttaaaaagcg cctcctgcga ggcggccaag acttccgcct cgcaggcccg ggcctccagg
    32221 tagcggagat atcgatcttc ttccctgata gggccctttt cccccgccat gacccacctc
    32281 cgatttataa taattttatt aaaacaaccg aggggcggcc ccgaagggcg cccctcggcg
    32341 cggctcttat cgaccggtga cgggataggg tctccggatc agttggtgag cagggagagc
    32401 atggcccccg cagccaccgc cgtgccgatg actcccgaca cgttgggccc catggcgtgc
    32461 atgagcagga agttccccgg atgctcctgt tggcacatac gctgcaccac ccgggccgcc
    32521 atgggcacgg cggagacccc cgccgaaccg atcatggggt tgatcttgcc accactccat
    32581 tttttcatga cttggccgaa gagcacgccc ccggcggtgc tcgccatgaa ggccacgagc
    32641 cccaggaaga tgatcttgag ggtggccacg gtgaggaagg tggcggcctc catggtggcc
    32701 cccacagtga gccctaggaa aatagtggtg gtgttgagaa tttcgttctg ggtcgcatgg
    32761 ttcaggcgct cggtgacgcc gcactcccgc aggaggttac cgaacatgag cacccccacg
    32821 agggggacgc acatggggag aacgaggccc gacgccacgg tgcagacgat gggaaagagc
    32881 accttctcca tcttggagac ggggcgcagg ttgtccatgc ggataccccg gtcctctttc
    32941 gtggtgagca gccggatcac cggaggctga atgaggggca ccagggacat gtagctgtag
    33001 gccgccacag cgatggcccc caggatctgg ggcgccatct tcatggtgag atagatgctc
    33061 gtggggccat cggcgccgcc gatgatgccg atggacgccg cttcccggat gttgaacccc
    33121 agggcggtgg ccaaaaagag cgccacgaag acccccatct gggccgccgc ccccagcagg
    33181 aaggtgatgg ggttcgccag cagagggcca aagtccgtga gggctccgat gcccatgaag
    33241 attataatgg ggtaaatctc atgttctgta ccgaaaaata cgtagcgcag gaagcccccc
    33301 tcgtccatga tgcccgacag gggcaggttc accagaaggc agccgaaggc gatgggcacc
    33361 agaagaagag gttcaaaacc tctcacgatg gccagataga gcaacacgaa ggacaccagg
    33421 agcatgacca cgtttcccag ggtgagcccc gcgaagcccg actgggcaat aaggttggac
    33481 agggcttgga agtacagcat cgccgattct cctcccgaca aaaaggggcc aggcccctag
    33541 cccacgacca cgagcacgtc gccggtgttg accgaatcgc cgtctttgac ccggatctcc
    33601 ttcaccgtgc cccctcgccc ggccaggatc tcgttttcca ttttcatggc ctccaaaatg
    33661 agcaggatat cgccgttttt caccacggcc cccaccgatg tggccacttt aaggattttc
    33721 cccggcatgg gggaagccac cacctcgccc cccgccacgg gagcgggagc cgccgcaggg
    33781 gccggagcgg ccaccggggc cggagcagga gcaggagcag gagcaggagc aggagccggg
    33841 gcgggagccg ccaccggagc cggggcaggg gcaggggcag gggcagaagg gggcatagct
    33901 ccgcacccca gatcctccac ggaaacatcg taggctttgc cgttcaccgt cacacgaaag
    33961 ttacgcgcca taagtagttc ctccttcgat cgccatcacc atgatccatc gggatcgcta
    34021 tgtatttttt aaaaaaccag agatgacccg cacctagcaa ggggcggtag gggctaatcg
    34081 agcccctcga tgttctggct ccgggccagg gccttccaca gggagccgct ctggcgcacc
    34141 ggccggatgc ccgtcaccac cacggggcgc cccaatgaca tcgccagggc ccccgtgatg
    34201 gcggccacta ggtcgccgtc gtccggcaag gccaccgccg ccgccatacg ccccccggat
    34261 ccatctcccg atggggcctt ggaggccgcc ttttctcggt ttctccgatc cacctgctct
    34321 gccaaatagc ggttggcata aatgatgccc atgagggcga tgagcaccag aaaaacgatg
    34381 ctgaaggcta taagcgaaag ggtgatggcg ccccccatgc ccacgaagtg cgatgcagta
    34441 ttcatgctga tctctctccc tttccggacc tagtgcggca tgacgccgtg cttccgcttg
    34501 ggacgctgct cctttttgtt ttccgtgagg agcagggcct cgtagatggc gccccgggtt
    34561 tcttcgggaa ggatgacccg atcgacgaag ccgcgcttcg ccgcctggta ggggttggca
    34621 aaggcatctt cgtactcgtc gatcttctgc tgccgcatgg cgttctggtc cgaggcggcc
    34681 tcgatctcct tacggaagac tatattggcc gccccctggg cccccatgac ggcgatctgg
    34741 gcctggggcc acgccaggac cacatcggcc cccaggtcct tgctgcacat gccgaggtag
    34801 cttcctccgt aggccttgcg caggatgacg gtcaccagtg gcaccgtggc ctcactgtag
    34861 gcataaagga gcttggcccc gtgccggatg atgcccccca gctcctgggc cgtgccgggc
    34921 aggtatccgg ggacgtcctc aaaggtgacg atggggatgt tgaaggcgtc gcaaaagcgg
    34981 ataaatcggc tcgccttgtc cgaggcgtcg atgtcgaggc accccgccag gttattcgcc
    35041 tggttggcga tgatgcccac ggcgatcccc cccacccggg caaaggcggt gacgatgttt
    35101 ttcgcccaaa ggggctgcgc ttccatgaaa ctcccctgat cgacgatggc cgagatcacg
    35161 gagatcacgt tgtaggactt gttcgggttc gtggggacaa ggtttcgcag ctccaggttc
    35221 ggggcatagg ggtcttcggt gcactccgcc ccgggggcct cttccatgtt gtttgacggc
    35281 agataaccca ggaggcgccg aatttcagca aaacactcgt cttcgttctt ggagaagaac
    35341 tgggccaccc cggacttggc gttgtgggtc atggcgccgc cgatctgctc gctggtgacg
    35401 tcttcccccg tcacggcctt gatgacggcg ggccccgtta tgtgcatgac ccccacctga
    35461 tccaccatga agacgaagtc ggtgagggcc gggctatata ccgccccccc ggcacagggg
    35521 ccggaaatga cggagatctg aggcaccacg cccgaggcca gcacgttgcg gaagaagatg
    35581 ttgccgtacc ccgaaagggc gtccacggcc tcctggatgc gggctccgcc ggaatcgttg
    35641 atgccgatga cgggagcgcc gtttttcacc gccaggtcca tgaccttgca gatcttgcgg
    35701 gcgtgcatct cccccagaga cccccccatg acggtgaagt cctggctgta gacgtagacg
    35761 atccggccat cgatggtgcc gtatcccgtc accacgccgt caccggggaa aacggtcttc
    35821 tccaggccga aattagtgca acggtgctcc acgaactcgt cgatctcggt gaagctcccc
    35881 tcgtccaaga gcttaaagat cctctctcgg gcggtaagct tacccttctc gtgctgtttg
    35941 gccacggcct tttcgccccc gccagccttc gccgcctctc gcttggccag cacctgctca
    36001 cagagctggt ctatggtctt ctccgacatg cgatacctcc tcacctagct caaagacacc
    36061 acgccaaccc cccgcgaccc aaagaccttc caggggatcc tcccgctagt ccctgtcgta
    36121 tcagtgactt cgctccgaaa gctccaacag cacccctccc gtggccttgg ggtgcacgaa
    36181 ggcgatgcgc gccccccccg cgccgtaacg gggcttctcg tcgatgagtt gcactccccg
    36241 ggccttgagc tcttccagag cttcctcgag gttttccacc cgcagggcga tgtgatggat
    36301 tccttcgccc tttttttcga taaactttgc cacggggcta tcgtcggccg tgggctctag
    36361 gagctcaatc tccgtgtctt tcacgggaag aaaggccgtg cgcaccttct gctcggccac
    36421 ttcctccacg cccacacagg aaatacctag ggcattttcc caaaactcca gggcttttct
    36481 aaggtctttc accgccacac cgatgtggtc caccaccgtc ggcttcatgc tccccctcct
    36541 ccaccggact gttttggcct cttatcgccc ttctctcacc ccgggcccca tatgcagacc
    36601 caatcctcgg taaagcagga aagggcgagg ctttgaggcc ccgctaaagc ctcatgcccg
    36661 ggattcccgg gccctttttt cctctaccgc cttctggagc cactctatag tggtggtggt
    36721 gggcgtaccg gggccgaaga ttgcccccgc ccccagatcc ataagcccgg ggatgtcacc
    36781 ctcgggaatc actcccccgc cgaagacgat gatgtcttcc gcccccttgg ccttgagaag
    36841 gccaatgatg gtgcggaaat agtggtcatg ggctccggag agaatgctga tgccgatggc
    36901 gtcggcatcc tcctggatgg cggtctctac gatctgttcc ggagtctggc gcaaccccgt
    36961 gtagatgacc tccatgcccg cgtcgcgaaa ggcccgggcg atgaccttgg ctccccggtc
    37021 atggccgtcg aggccgggtt tggccaccac gacacgaatc ttgcgatctt ccatggaaat
    37081 cctgcctcct tcacatgacg aaacggaaca agtcagccct cgcctccgac ctcggaggcg
    37141 attctacggg gagctacagg acgatgtttt cccggtactc tccgaactcc tcgcggagca
    37201 ccccgcagat ttccccttcg gtgccgtagg cccggacgca atccaggatg cggggcatga
    37261 ggtttacgct ctcgtcccgg gcggcggcac ggatctcatc caaggtggtc tgtacccgga
    37321 ggttatcgcg cttggccttc acggcggcga tcttttccgc ctggcgcctc cccacgcgct
    37381 cgtcgacccg caagagctct cggccggagt tatcctcttc catctggaac ttgttgaccc
    37441 ccaccaccac catgtctccc gtctcgatgg cccgctggta ggcgtaggcc gcgtcctgga
    37501 tctgctgctg cacgtagccc ttctcgatgg ccgtcaacat gccccccatg tcgtcgatct
    37561 tggcaatgta ggtctccgca tcctggcaga gccgatccgt gagggactcg atgtaatagc
    37621 ttccggccaa ggggtccacc gtgctgcaga cgccgctctc gtaggccacg atctgctggg
    37681 tgcgcagggc gatgttgacg ctcttttccg tgggaagggc cagggcctcg tccatgctgt
    37741 tggagtgcag ggactgggtg cccccgagca ccgccgccag gcactgaaag gccactcggg
    37801 cgatgttatt ttcgggctgc tgggccgtga gggtacaccc cgccgtctgg gtgtggaagc
    37861 ggagcatcat ggccttgggg ttggtgatgc cgaagcgttc cttcatgagg cgagcccaca
    37921 ggcgccgagc ggcgcggaat ttggccactt cctccaggaa gtcgttgtga gagttgaaga
    37981 agaacgaaag gcgctcgccg aagacgttgg ggtcctggcc ttttttgatc gccgcatcca
    38041 cgtaggcgat gccatccgcc aaagtaaagg cgatctcctg ggccgccgtg gatcccgcct
    38101 cacggatgtg gtacccggag atggagatgg tgttccattt ggggacgtgg ttgctgcaga
    38161 attcaaagat gtccgtgatg agccgcatgg agggcttggg agggaagatg taggtgcccc
    38221 gggcaatata ctccttgagg atgtcgttct ggatggtgcc ggagagctct tcggaacgca
    38281 ccccctgctt ctcccccagg gcgatgtaca tggcaagcaa caccgacgca ggggcattga
    38341 tggtcatgga cgtggagacc ttgtccaggg gaatctggtc gaaaaggatc tccatgtccg
    38401 ccagggagtc gatgggaact cccaccttcc ccacttcgcc ctgggccatg gggtggtcgg
    38461 agtcgtagcc gatctgggtg ggaaggtcaa aggccaccga aaggcccatg gatccttgct
    38521 ccagaaggaa gcgatagcgg gcgttggagt cctccgccgt ggagaacccc gcgtactgcc
    38581 gcatggtcca aaaacgcccc cggtacatgt tgggctgcac gccccgggtg aagggatact
    38641 gcccggggaa gcccagatcc cgcatataat cgatattttc cacgtccgca ggggaataca
    38701 ccggggccac agtctcgctg ctggtgcgga actctttctt ccgctccgga gccttggcca
    38761 acatcttttc catggtcttg cggtgggctt ccgccccttt tccgatggcc tggagctgat
    38821 cttccttgaa caccgtttct ccctcccgac tgaaaaacaa aaaagtaaca ccctggcaaa
    38881 actgctgccg ccggaaccgg cgactttact catcgtcacc cctcggggcc attatttccc
    38941 agagtgcgga aaaaggcaac tacatagcgt tattttcgga acaattatac acaaattcct
    39001 ccaacgcccg ccccgtggca catcacgttt tttccctccc ccgtactgta catccggggg
    39061 gtgtccctgt ccagagatcg cccccgcccc gggccctaga atactgtatg aaaagccctt
    39121 cgtctccttc gcctcccccg atggtccccc gggaccggcc cgtcgcccct aggagggcgc
    39181 ctatatcacc tcttggtcca tttctccacc atctccggag ggtcttccct cgctatcatc
    39241 ggcgtgctcc tggaaaattt ggaaaaaggc atcccctgag gccaggaaga tgtccaccaa
    39301 ccgggggtca aagcgcgtcc cccgtccctc cgcgatgatt tccatcgcca catggtgggg
    39361 aaagggctct ttatacggac gccggctcac gagggcgtcg tagacatccg ccaaggtcat
    39421 gatgcgagcc gaaaggggga tctcctctcc cgccaagcct cggaggtatc ccttgccatc
    39481 ccagcgctcg tggtgatctc gggcaatgtc ctgagcatgg gaaagaaagg tgttccctcg
    39541 aagccttcgc tccccatccc gcaggatctc gtacccgtag agtgtgtgat atcccatgac
    39601 ccggaattct tcgtcggtga gcttgccggg cttgagaagg atgccgtcgg gaattcccac
    39661 cttgcctatg tcatgtagcg gggccgtgga ggcaaaaagg gctatggtct cgggggaaag
    39721 gaccccggca aagtcggggt gcgacgccgc ccgctccgcc aggaactgga cgtagcgccg
    39781 ggtacgctgg atgtgacccc cggtctccag atctcgccgc tccgccatcg ccgccaagct
    39841 atcgatgatc acctcgcggg tgagctccag ctcccgggtc cgatcccgca ccgcatcttc
    39901 aagccggtct cggtggtgtt tcagctcgag ctggttgcgc acccgagcct tcaccagggc
    39961 ggggcgaaag ggcttggtta cgtagtccac agcccccagc ttcaaccctt gggcctcgtc
    40021 ttcttcctgg gacagggcgg tgagaaaaac cacggggatc ggcgccgttt tcggatcggc
    40081 cttcaggtga gtgcagactt cgtacccccc catgccgggc atcatgacat cgagcaagat
    40141 cagatccgga ggagcctccc cccgggccag gcgcagggcc ttctcgcccc ccgtggcggc
    40201 caccagagaa aactcgtcct ttagcgtttc cataaggatg tggatgttct cggggacatc
    40261 gtccacgatg aggaccttaa aacgcttttc tctatctcct tccacggggg aactctcccc
    40321 tttcttcttt tttcttcttt cttcgttctg gactataacg cccggagctc atgacaggag
    40381 cttcaccagc tgatccagga cggaggcggc cccatcggtg tcgaagtggc gcaacatccg
    40441 gtcgagggtc tgaaagagat cttctccttc ccggcccgcc cggcgccggg cctcggcgat
    40501 ccgggcctcg gccttgggaa ggtcgtcgtc caagagggcc ctcccctcct ccaggagccg
    40561 ccgggcctct tcccgatcct cgaggagccc catgggctcc gcctccggat ccccttcgac
    40621 ctcttcgcct aatagggcca ccagctcctc gcgcaccgcc ctccactctt ccaacacgtt
    40681 atccttggcc tcttccacga cctccgacga cggggagtcc agggcccttt ccagggcccc
    40741 cgctactgcg gccagatcgt ccaaggccag gttccctgcc gtgcccttga gggtgtgaat
    40801 ccggcgccga agctcatcgg agggctccgc cccgcttcct ttcagggcga ttcccagatc
    40861 tatctgctct tcgacgaata gccgaagaag atggcgatag aacgcctcat ccacccccag
    40921 gcgctccgcc cctcgggcca gatccactcc ccccgcaaaa ggaggtggcg ccgcccctgc
    40981 gacttcttcg ggccccgacg ccgagaggac ttttttctcc ggccggagga tccacctttc
    41041 catggtctca aaaagcccat ccgggtctat gggcttggtg acgtggtcat tcatccccgc
    41101 ctccaggctg agcccccgat cccgttccat ggcatgggcc gtcatggcca ccagggggat
    41161 agtctcccaa gcccctccta gggcccgaat gcgccgagcc gcttccaggc cgtccatctc
    41221 gggcatctgg atgtccaaaa agaccaggtc aaattcccgg cgggaggccg cctccagagc
    41281 ctctcggccg ttcgtggcca cggtcaccac gacgccccgc ccttccaaga tctcccgggc
    41341 aatctggcga ttgatgtcat tgtcttccgc cagaaggacc tgcgtcccct cccaccgttc
    41401 cgaaacgccg cccctggggg agggggttcg ctcccggagc ctgaggtcgt cgcggcccag
    41461 ggccgccagc acggccccga ggagggagga gggtaggacg ggtttaatga ccgcttcttc
    41521 cctagttttc ttaccgccct tggatttttt tcggtcttcc gccagggata ccagggatac
    41581 caagggaatg gggcccgagg gtagctcttg aagagcccgg tgcagcgtgc catccgccac
    41641 ggcccggaga tcgccatccg ccacgaccaa tcgcaccgga gcgcctctat cctccgcgag
    41701 ggtctccaag gcctcttcca ctccggaggt ggagatcacc tcgaaagaaa gcgacgtcag
    41761 catccgggag atcactcccc gggccacagc attgtcatcc accaagagca ccttcaagct
    41821 ccgcagctct tccggaggag aaagaggagc ctcttgggcc tttcccaact cgagcagtgc
    41881 ggaaaattcg aaggtactcc ccactcctgg ggtgctcgaa acttctatcg tcccccccat
    41941 catctccacg aggcgtttgc agatggcaag ccccagcccc gtgcctccgt aacggcgagt
    42001 gatggacccg tcggtctggc tgaaggactg gaaaagagcg ccaatcttgt cctccgaaat
    42061 gcccaccccc gtgtcgcgca cggcgaacga aagcaccacc accttcggag accaatcctc
    42121 cagggccacg gtcaccacca cctcgcctcg ctcggtgaac ttcaccgcat tggtcaccag
    42181 gttgacgagg atctgcccga ggcgcaaggg gtccccttcc agggagcggg gtacgtcggg
    42241 acgggggtcg aagagaagct tgaggccctt ctcctgagcc cgtagggcgg tgacggtggc
    42301 cagatgctcg aagaccgtgt ccaggtcgaa gggcaccgac tccagggtca ttttccccgc
    42361 ctcaatcttg gaaaaatcca aaatgtcgtt gatgagcccc aacagggctt cggcggcgcc
    42421 ctgggacttt tgcaggtagt cccgttggcg gtgagaaagg gcggtggtgc tcatgaggtg
    42481 ggccatgccc aaaatggcgt tcatgggagt acggatttcg tggctcatgc gggccaggaa
    42541 atcccccttg gcccggtcgg cgctctcggc cgccctgcgg gccctttcta tttcctcctg
    42601 ttgcgccttc agttccgtga cgttggtggc caccagggcg gtgcgacatg gggctgattc
    42661 atcgcgcacc aggaaaaaac tcgccaggac ttccttcgcc gccccgaagg ggccccccat
    42721 ggtcaactcc cccgaccact cccccgaggt ctccagctcc accgccacct cccgccggat
    42781 gcggtccgcc gaggaggggg cgcaaaagtc ttccaggagg cgcccctcca ccgtctccaa
    42841 attctgcccc acccacctca aaaaggtggt attggcgtac tgcagacggc ggtccgcatc
    42901 cgccacggcc acggcctgac ccgaggaatc caccagcagg cgaaagagcc ggagttcttc
    42961 ctgggatcgc aggcgaaggg tgacatcttc cttcacgatc acgaagtgag tgatgcgccc
    43021 atcgccatcc gccaccgggg cgatggacgc atgttcccaa tactcttcac cgtttttacg
    43081 gcggttggga tagtcccccg accaaactcg ccccgccagg atcgtttccc atacctcttc
    43141 ccaagggctc accgacgggc ggtcgccgtc aatggattcg atactctttc ccaggagctc
    43201 cgcaaaggtg tatcccgtca tctcttcaaa cttgggattg acgtactcga tgctcccctc
    43261 ccgatccgtc accaccacgc ccatggggct ctgctccgtc acccgcaaga gcttgcgaag
    43321 gtttccctcg ctctccttcc ggtgccacac ctcccgcttt agcgcttcgt tggactcctc
    43381 cagatcggcg atcagggcgt taaaggcccg agccaccacc ccgatctcgt cgtcgcggtc
    43441 gtcggagatc agcttagcca cgccttcgcc ctctcgccgg ggcgcccgaa gttgggccgc
    43501 caactctccc aagggacgca ccaccacgtg gcgcaggagg atcatcatgg cccccagcga
    43561 gacgatcccc gtggccagga tggcgacaag ggaagatcgc accatgcctt ttcccgcctg
    43621 gtggaagggg cgggaaaact ctccccgcag gacccccacg ggaactccgg agatgtcttt
    43681 gatcagggcg taccccagga gcaggtcgtc ccggctttgg tccagggcca tgccgtcctc
    43741 cgcccggagg agctcttcga agatcttccc ctccccgggg tcgatataga cctgggagga
    43801 gctctggcga cgcaagtttt ccaccgcctc ggcatccacc actcttccca tgacgcaggt
    43861 tccccgggga acgccatggc cgtcgctgcg gatgatggga cgcgacgcca ccataataag
    43921 gccctggggg gtcttcagaa ttccctggca aggaggcgcc aaggcctcat aaagcccctc
    43981 caggaactcc ggaggcaagg gacccttcat aaaaaccgga gaaattagtt tttcctccaa
    44041 cgccccggcg gcatcgaaga gaaggatcag atcaaccccc gaactctgca aggcgccgtc
    44101 gtggaggttc tcccgccgga actcggggga gtgatcctcc acataagcat agagggaatc
    44161 ccaggtggcc cagtcccgac acgtggtgtc caggtggccc acttctactt ggataatggc
    44221 ctctatgcgg tgcaacgccc gccgggcctc gtccctttcc agggatcgat ggctttccat
    44281 taccataaag ttctccctca cggcaaagat tccgagggaa aaaatcacca ccgccgtcag
    44341 ccccagcata attttccggg atatggtcat cgcgagagcc tccccgcaga acatggcccc
    44401 attgtacaac ggagtcgagg gaaaaagacg atgacggacc aagttttctt catggactat
    44461 cccggagcca aggacgcgcc ccgaggcctc attcccctgg ccctttgcgg ctcccgggct
    44521 tcaccagagg aaggcccgca gcgcacaggg ggcactcttc gggatcgtag aggggaaaag
    44581 ccgccacgag gaggctggtg agatcgtggg gcagccccgt cttccctccg ctgcgatccg
    44641 ccacgcaggc cgatcccacc cactccgccc caccggtggc caggtgttct cccacctccc
    44701 ggagggaccc accggtggtc accacgtcct ccaccaccac gaatctcacg gcgccggggt
    44761 ggggaaagcg tcgcaagacc atgcgccctt cctgacgctc acagaagaca aagggaaccc
    44821 ccaaggcccg ggccacttcg tgaccaatga tgaccccccc caaggccgga gacgccacca
    44881 tctggggccc caggggggcc aagagcctcg ccagcccttc cccgacgaag gccgcatgcc
    44941 ggggatgctt caggagcagg gcgcactgca tgtagtgatc gctgtgcttt cctgaagtta
    45001 ggaggaagtg tcccgatagc agggctccgg tctccttcat gatgtcttct aaaccattca
    45061 tcgctcgcaa aatgcccatg ctacgcctcc tcgatctctc tgtggacggc ggccagggcc
    45121 gcctcggggt ccggggcctc caggagggga cggcccatga ccaagtagtc cgccccgtcc
    45181 cggaccgccg tggccaccga cgccgtgcgt cgctgatcgt cttccccccg ctgaggacga
    45241 atgccgggaa ccaccttgag tagccgatcg cccccttctt ctcttaccag cgtcagctct
    45301 tggggtgagg acaccaagcc gtcgaggccc accgcagcac agagccgagc ccgggcggcc
    45361 accgcccctc gcacccctcc tccggcggga caaacttcgc cccagagatc gtcatccagg
    45421 ctggtcaaaa cggaaatgcc aagaagcttc atgacgctct ccgccccgtc ccgggcgtcc
    45481 cgggcctggg agagcatctc ccgccccccc gcgctgtgaa gggatagggc ccagagcccc
    45541 atccgggcaa agacgtccac cgctagggcc acggtgttgg gaatgtcgtg gagcttgagg
    45601 tccagaaaaa tgtcgtagcc ccgggattgc accgcctcga taaagtcccg ccctcccagg
    45661 gcgtagagcc ggggcccgat cttcacgtgg cgaagccccc ttcccacgcg atccagcatc
    45721 gcctgcccct cttccaaggt cctggtgtcg agggccagaa tgacttcggc tttcctttgc
    45781 acggcccacc tctcctttct aggtctaaga tctcctgttc atcccagggc tttaggcccg
    45841 ccgccccgcc cccaccaggg aggagatggt gtccaggcca aagcgctcca gatgtccttc
    45901 gacggcctcc atcatggcct tggccgcccc cagatccgcc atgagggcgg tgcctacttc
    45961 caccgccgag gcccccgcta gtagcatgga gatcacatcg tttcccgtgg tgacgccgcc
    46021 acaccccacc acgggaatgg atacgctccc cgccacgtcc cagacacagc gcagggccaa
    46081 aggaaatatc gcaggccccg agagtccccc cacgaccctt gccagggccg ggcgatggcg
    46141 ctccacgtcg aaggccatgc cgagccaagt gttcgccacc accaccgcat cggcgccccg
    46201 atcttccgcc gctcgggccg tggcccccag gtcggcggcc tggggggtga gcttgaccca
    46261 caggggcccc cgccaccggc tccgggccag ggccaccgcc cgggataccc ctttcgccgt
    46321 cactccccaa gcggcgccgc cgcaggccac gttgggacag gagaggttca gctccaccgc
    46381 cgccagatgc tccgccccgg cctccagtgc cccgaaaagg ggatcgaagt cttcctcggt
    46441 caggggggcc acgttggcca ccaggggcac cccctcccgg gccatctcgg gcaaatccct
    46501 ttcgacgaag gatcgcaccc caccgttttg gagccccaca ctgttcaaca cccccgcagg
    46561 ggtctcccaa aggcgcaccc ccggatttcc ttcccggggt tccagggtga tgcccttggt
    46621 gcacgccgcc cccacgaagg ggaggggctc ccggggccag aggtcccttt ccaagggcca
    46681 gacccccgag gcaacgatca acggagaatc taggatcacc cctcccaccc gggtttgcac
    46741 ggatctcttt ctcatgagcg tccctccaac tcctccaggg ctccccagtc cacgtcccgg
    46801 gcgtcgaaaa aggggccctc cacgcaggcc cgcacggggc cccgtaccgt gggcacggtg
    46861 catcccaggc agccgccgta gccgcacccc atccgctggt ccaggttcac caagaggcgc
    46921 atctccgggg gggcgcagcg ggccatcgcc cggatcatgg ccgggggacc gcaggcccag
    46981 acctctcccg ccccctccag ggcgtcacag ggggtacccc gctcgcccag ggatccgtcg
    47041 tccgagaaga cgcgcaggtg cttacccact cggtgggcca ccagccgctc caggggcgcc
    47101 caggatccat cgggaacccc caaaaccacc gtggggacct caccgcgccc cgcccggagc
    47161 tcgtcccagg catacatcag gggggccacg ccaaagcctc ctccggccaa caccagggga
    47221 gcctgggccg ggggagggaa gggagtcccc aaggggcccc tcagcgtcac ctcggcgccg
    47281 gggcgcaggg ccaagagggc ccgagagccc cggcccaggg cagccaccat cagggtgatc
    47341 tggcttcccc gcaccgccga cacccccaag gggcgtccca gaagggggtc ttgccaggga
    47401 gaaacccggg ccatgagaaa ctgccccgga cggagctccc gggccaggcg aggggcttcc
    47461 aggataactt cccccaggcc gggaatcagg gggcgccctc ccaccacgag ggcgcgctcc
    47521 tccagagagg actccctact catgaggagg ctccgaaagg gcgtcgaaga ccaaggcccc
    47581 ggccaccatc gtggccaccg gggcgccgat gaagcgcttg ccccgatacg gggtgaggcg
    47641 caccttgctg cgccattgat cgggatttac ctccagggga aggtcgagat ccaacaccgt
    47701 gaggaaggcg ccccccccct cctcgattcg tccggggacc ccggcccagg gcggaagaag
    47761 ggcggcgggc cccgaggtcc acaggcgcag aagggcctcc aagggaacct tacggcgacg
    47821 cccttcgtcc agtaccgccg ccacggcgca ctcgtaggag gcgatgccga aggaggcctc
    47881 gacgaagggt aggtccttct catcctcgtg ccagggggca tgatccgtgc ccagggcgtc
    47941 caccgtgccg tcggcgacgc cctcccagag ggcttccacg tctcccaggg atcgcagggg
    48001 aggattcact ttaaagatgg cgctaagatg gctcgtcagc acgttttctt cggaaaaaat
    48061 cagatggtgg ggcgtcacgt cgcaggtcac cgggagcccc tcccccttgg cccgacggat
    48121 ctcttccagg gcctgggcgg tgctcacgtg ggtcaggtgg accgcgcagg aggtctcccg
    48181 ggccagggcc accgcccggc cgacgccgat gacctccccc gctgcgggca tgcccctcat
    48241 gccgctcagg gcggaacagc gcccctcgtg cacctgggct cccttggaga ggtcggggtc
    48301 ctcggggtgc tccatgatcc gcacccccag atcggaggcg tagagcaggg cgtggcgtag
    48361 caggctcgac cggagcaccg gggctccgtc gtcggtgaaa agcaccgccc cggcctctac
    48421 catggaggcc agttccgcca tttcctcacc acttcgaccc cggctcacgc acccggcggg
    48481 aagaatcagg gcccccccgg tgtgccgccc tcgctcccgc acgtcccgca ccagggcttc
    48541 ggagtccagg ggaggttcgg tgttaggcat ggccaccgcc acggcaaatc cccccgccgc
    48601 cgccgcccgg gctccggaag tcaggtcttc tcgccaggtc tgcccgggat cccgaaagtg
    48661 tccgtgcagg tcgacaaagc cgggggccac gacgcgcccc gccaggtcca tcacccgggc
    48721 tccctcggag cccgaaaggc ccgaagcgat gcgcgtcacc gtctttccct ccaccaagag
    48781 gtccgtcgcc cctccgtgga gctggacccc atcgaaaagc cgggcattct tgagaaggat
    48841 catgcttctc cacctcccat gcaaaggtac agaagggcca tgcgagtggc cacgccgctt
    48901 ttcacctggg gcaagatcac gctctggggg ccgtccgcca cccgagattc gatctccact
    48961 ccccggttga tgggccccgg gtgcatcacc agtgccccgg ggcgggcccg gcgcaccagt
    49021 tcctcgttcg ccccccacag gcgatgatat tcgtcctcgg aggggaagag ccccgccatc
    49081 tggcgctcca tctggatgcg gagcaggtag atggcgtcgg ctcccaccac ggccctccgg
    49141 gcgtcgggtt cgtactccac ccccagggcc cccatgtcct tcggcatgag agatcggggg
    49201 ccagagcaca ccagagaagc ccccatggta cggaaggcga tgatgtcact gcgggtcacc
    49261 cgactgtgga ggatgtcccc cacgatggcg atgcgcttgc cctccaggga tcccagatgg
    49321 cgccaggcgg tgtagacgtc gagcagggcc tgggtggggt gggcgtgggc cccatctccc
    49381 gcgttgaaga ccgacgcccg ccgaagctta tctctaagat accagggcac tccgggcatg
    49441 gagtgacgca ccaccaccgc atcggccccc atggcttcca aggtccaaac cgtatcccga
    49501 agggtctctc ccttgctgac gctagatccc gatgaggccc aattcaccac gtcggctccc
    49561 agaaacttct ccgccagttc aaaggatacc cgggtgcggg tagaagcctc gaaaaagatg
    49621 ttgaccacca gcttgccgcg cagggcggga acctttttta ccgggcgatc catgagatct
    49681 tccatgtgca tcgcctgatc cagcaaggtt tccaaatcct cccgggtcaa cacatccacg
    49741 tccagcaaat tcctgtgact ccaagccata gactattccc cttccctggc aaaacgcatc
    49801 aagggccttt cgggaccttc ctcgaccttt tcgaccttct cgcagatcca gacctcgtcc
    49861 accccgtcca cttcctgcag gcgtacctcc accacttccg aagacgacgt ggggaggttt
    49921 ttacccacgt agtccgcgtg gatgggcaac tcccgatgtc cccgatccac caacaccgcc
    49981 agctgcactc cggcgggacg cccgagatcc ataagggcgt ccagggcgga ccgcaccgtg
    50041 cgcccggtgt aaagcacgtc gtccacgagg acaacccgcc ggtgggtcac gcccccgggg
    50101 atgtccgtgc ggcgcaccag gggttgggag tagcgcagcg acaggtcgtc tcggtacagg
    50161 gtgatgtcca gttccccctg ggcgggacgc atcccttcaa agccctccag cagcgccccc
    50221 aggcgcgacg ccaggggaac gccccgacgc tggattccca caagcacgat cccttccaac
    50281 cccttgttcc gctccacgat ctcgtgggcc atgcgcttga gcgcccgggc catgtccgga
    50341 gactccatca cccgcgcctt ttccttccaa ttcatgggtg gacctccttt cgggataaaa
    50401 aaaatgggcc tcctctcccc agggggaaag aacgcccacg gataatcctc atcgcggccc
    50461 agggccgaca cgccttttgt cccatcttca ccgcctcctt ccgggcctcg cggaacccga
    50521 ttaaaggtga cccgacgctt cgcaggggac tctaccacga aacggcggtt ttggcaactt
    50581 cccccgggag gatcctcctc aggactcggg agggcggacg ccagtcccgg ggcgcgaaag
    50641 cctagtacgc tttggcgaag atgaccctcc gggctccttc ctggccggtg tagaagcact
    50701 ttcccgtgcg ctggtccgcc agggggaaac accggggagt ggcgccggtg gcttctttga
    50761 tagccctttc gtcttccttg ctgcccccga aatatcccgt gataaatcct ccggaggagg
    50821 ccagaacctc ctggaactcc gcgaaggaag agacctcgtg ggtgttatcc tggcggaact
    50881 tcagggcctt ggcgtggagg tcgcgctgaa tgcgccccag gagggcgggg acttcttcgg
    50941 cggcccgatc caggggaaag gatccttttt cccccgtgtc acgccgcaca tatcgcaggg
    51001 tgccttcctc cagctcccgc tcccccaact ccagccgcag gggaacgcct cgctgaagat
    51061 ggtggaagaa gcggtccgca ggccgaagat gaaactgagt gtctatcaaa acccgccggg
    51121 ggccgagcac accgttgagc cgggcttcca cttcccgggc ccggggaagc aggatttcgt
    51181 ccatcctccc ctgatcgttg gataggggga ggatcaccac ttgggtcggc gccacccggg
    51241 gaggcagcac gaggccgtcg tcgtcggaat gggtcatcac cagggcccca atgaggcggg
    51301 tggaggctcc ccagctggtg gtccaggcgt attccaattg tcccgcctcg ctctgaaact
    51361 ggatgtcgaa ggccttggcg aagttctgcc ccaggaaatg gctggtgccc gcctgcaagg
    51421 ccttccggtc gctcatcatg gtttcgcagg tgtaggtgtt atccgccccc gggaagcgct
    51481 ctccttcggt cttctcaccg ggaatgacgg ggagggccag ctcatcttcc atgacctccc
    51541 ggtacacttc aagcatccgc agagtctcct ccacggcctc ctcccgggtg gcatgggccg
    51601 tgtgcccttc ctgccacagg aattccgacg tccgcaggaa gagccggggg cgtttctccc
    51661 agcgcatgac gttgcaccac tgattgatca ggatgggcag atcccgccaa gactgcaccc
    51721 atttgctgta catgtggcca atgatggtct ccgacgtggg gcgcaccacc aggggctctt
    51781 ccagctcctc tcctccggcg tgggtcacca cggcgcactc cggagcgaag ccctccacgt
    51841 gtttggcctc tttttccaga aaggagtggg gaatgagcag agggaagtag gcgttcacgt
    51901 gccccgtctc tttgaagcgg cgatcaaatt gctcttggac gtgctcccag agggcgtacc
    51961 ccgtggggcg caccaccatg cacccccgca cgggggcgta atccgccagc tccgccgccc
    52021 ggatgacgtc caagtaccac tccgaatagt cctcatctct ttttgtgatg ttttttgcca
    52081 tgaccgtccc cctcttcgtt tagccccccg gaggtcctcc gagggtgtga ttccaccatt
    52141 tttaagggat gatctcccgc cgcctaaagt cggaccccag ttcaaacccg aaggtcacgt
    52201 tgccgtcgcc gtcgtagatc accaggaccc gtccccccag caattttccc ggcaccgcca
    52261 gggcgaggcc cgcctcccaa ggggaggcca cttcatccca atcttcgtcg aacagggctc
    52321 cccgcgtcac gaagaggtcg ccgtagatgg gcccgaggag gacgctctcc cagagcatgc
    52381 gccggatccc caggcgcacc caggcggaac gctctccccg ccagggagtg gaggccagat
    52441 gaaagaggtc cttgggcccc cccaggtagg cggcgtgagc gggggagctg gcgtctccgc
    52501 cctggacccc tccggagagg agaaggcggc tctgggcccc ccagggctgg acccacagca
    52561 tatccgccgt ccacagaagg gcatccaggt tcgaccacca gaggttcccc tggaaccgga
    52621 ccccctcggt gggatcatcg gggttgtcca aggtggacca ggacaaggag accttgggcc
    52681 cccaggcgga ctgagtttcc ccgccgtatt ccaccctctc tcccacgatg ccccagccaa
    52741 tttccatgcc atcgcgccag aagaagcgcc gcacccccgc cacgtaccga tcccaggcct
    52801 ctcggtccat gccgtggggg gtgacggttt cgctccgggc ctccaggtag tactccatgc
    52861 ggcggaagcc ggaaaccggg gcgaagtagt gcaggccagc gccccaatcc tcccccactc
    52921 gcaactccga cgtcaggatg tctccctcgg cccagacccc gcgctgaacg ccctccaccg
    52981 cgagccaccg ttccggagcc aggttggagg tgtatccccc cacccgaatc tccacgtcgg
    53041 gggcggggtg cacctggagc accaccgcca cctgccccac cccagcgtcc tccaggcggt
    53101 actccaccac ccccacgtca tcgcgttctt tgatggtctc gcaggcgtcg agcacggcga
    53161 cggcgtcgaa ggggcggccg acccaggaag agtaggagtg ctctaaaaag gccccggtga
    53221 ccggagaaag gccctccacc cgcaccgccg ccacggtccg atcggctctg gcggggcgag
    53281 gaggcaccgg gggggccgcc gcagccagcc ggaggagccc cgaaagctcc tgttccgccg
    53341 cctggcgccc cagggccacg atctccccca cgtgccccga ctccaacatg gggcggtctc
    53401 ccacggcggg gcggatcacc aaatcggcgg cccttaggtt atccaggtag ccctgccggg
    53461 tcatgatggc cgtgctctga tcgaagacct ccaccgcgtt cctcaggcgg gaggcgggtt
    53521 ggagggcgct cgatacgtcc accgccacca cgggataccc gggaaagacc tctttcgcga
    53581 tgtggacggg gaggttggcc accagccctc cgtccagggc catgcgcccc tccacgggcc
    53641 agggctcaaa aaaccccggg atggccatgg aggcccgaag ggcggaagcc aggcttcccc
    53701 ggcggatcaa cagggtttcc cccgtttcca tgtcggtagc caccgccacg aaggggatgg
    53761 gaaggtcgtc gaaattaccc tctcggacct gggccgtgag ctgatgtagg gtggacaaaa
    53821 ggtgctttcc ctccatgatg cccaggggac ccgtggagcg ccctcgacgg tcgaaggtgc
    53881 ggcgaaagag gctgtgcacc tcgccgttcg ccgaagagga ggcgttgcga tcgtccaaca
    53941 gggccgggag atcaatgccc cgcaccacct cttccaaaga ggctccgtcg tatccggcgg
    54001 ccctaagtcc cccgatgatg gatcccatac tggtgcccac gatgcccgcc acggggatcc
    54061 ccgcccgctc caacacctgg atcactccca cgtgggcgaa gcccttggtg cctccgccgg
    54121 aaagcgccag gactaccccc ccgggttctc cccacgccgc cccccccagg accaaaagcc
    54181 ccacccccaa ggccaggaag cccttcatcg ccctccaccc catacgccac acctccggat
    54241 tttttgcccg agtcgtcccg gacccggttt gaagacgacg ggggctcggg gaatatgtca
    54301 ttttattttt aacgaacatc acctatcccg ctcctccctg gcaagggagg gctttaggaa
    54361 aaaaccgccc cgccagtata ctcgcaaacc aagaaagggg aaatcccgga ggagatgacg
    54421 acggtcccgt tattttaacc actgagaaag tctccccggc ctcgggcgaa gggccatgcc
    54481 gagccaaggc aaagcgccgc gatgggccag atcggtcggc ccttcgcggc gcttcgatct
    54541 ttttgaaggg acgtccccat cctaccccca caaaaagtaa aatagcagca ccaccatcaa
    54601 ccccaccaat cccacggctc cccagagaag ggctctctcc gacaaggggg gtttgtcttt
    54661 ttcttcgtcg tagggcgata gggcgatctt gatatccccc ggcgccacgg ccttgaaaaa
    54721 ccccttgtct ccccccaggt gaccgtagat ctcaaactgc ccgtcgcgca tgacccttat
    54781 taggtacacc cgcccaggga tggggctgcc tccaatttcc tggagcgaca tcatgtcccc
    54841 cggcaccacg gaagagaggg gaacccctcc caaggggtcc accacgggat cgagctcacg
    54901 cacccccgcg ctggggagta tgagcgatcc caggccctct cccccaaggg cttcagctcc
    54961 cccttctttg gaggaaagat cggcgttgag gtgaaatccc aaaaacttgt ccaggctctg
    55021 ctggagcgcc gccacctcaa ggcccagatc ccgcaggtcc ggcagggaaa ctcgggaaag
    55081 gcgcagggcc gtaaggggag agacctttcc cagcgattcc cgaaggtact gctcaatcac
    55141 cgcagagaaa cggcgcgtca tcccggagtc ctcgaagtat ccgtcgtcgt cctcctggtc
    55201 ggaccccttc tccgccatgg cgctttccag ctccgggacg atccccgccg cgaggccggg
    55261 ccacgggact ccgtacttgc gcgccacctc ctgggccctc accacctcgc tctccgccgg
    55321 attagtgctc cccagggagg cctttcccga cgccacctct atcacccggt gccagagccg
    55381 aaaggtggcg ctatcccggc cgtggggcac cgccgtcgac gcccaccaca ccacttcgcg
    55441 ccccggcccc atcaccgcca ccgccagaat gctcaactgg tccgtgaagg gagataccac
    55501 ccagaggacg tggtaccgac gggtctgggc cgccaggaag gaaaagaggt ttctcggatt
    55561 atccgcccct ccatcggagg aaaattccgc caccagccgc gaaacttcct cctggggcac
    55621 ccccaggagg taggatagca agtccagcag gctggaagtc tgcgccgcgc ccgtctccat
    55681 ccccctcgcc tcctcatcgg tcttcggcga tcctcgcacc gaccccgcgt catcccaaag
    55741 gtctttctac ggccagagat cccccacctc caccgaaggg ctgccctcga cggcgccccg
    55801 gcgcaccccg cagttccaaa aacggatgcc tgggaagagg atgtgctgcg aagggctcta
    55861 ccggcctcgc ccggagggct ttccccggcg gagcgctccc gcaggcggac cttcgtgggg
    55921 aatctatccc ccgaggtcga tggccgcctc actcttcctg gtaaatggat atgaccagaa
    55981 acaccgtccc cgagggaacc acggaaaagg ggagggtgaa ggacttgatg gcgggcttct
    56041 ggggaggcac ggccttgatg ttttccccgg tgattacctg ggggggcgtc aaatccaccc
    56101 cgtcgatgtc gagggccatc aaggtctggc cgttgaccat gttcgtcagc tcggcgatga
    56161 ccgagagggc cacggggtcc tccagatcgg ccgagatcat gcctcctccc atggcgttga
    56221 cgtaggcggc aaatccccct tcgtccgcca tgatggacga tgtgccgtgc acccctcccc
    56281 ccacgatgcc caccaccgcc gccacccggc ttcccggcgt gttgactcct tgggcgatat
    56341 gggacttctg gagctcggct ttaactccca gggtagagct cacggagacg agcgccgccc
    56401 cgttggcgtt gacgaacttc gagagaatct gctgactcac gggtattcct cctcttatag
    56461 gaccggggct ctggctcacc ggaacaatcg gtttctcgct ccttttttct ttaggatctc
    56521 ccgatctccc gaggtacggg gcctttttta ccccacattc ctggggaaaa cccccgggta
    56581 cggaggcttc aaagggtcgc gggggtccct cggccttttt tcccattcta cacgctcagg
    56641 aatcttttca aaaggggaca acttctcctt tgtgatgaaa actacggtaa ggggggcttg
    56701 agacccgggg aaagggttgc tccccctctt tgggagctag ggggcctccc ccaaaaaacg
    56761 ccgcacgagc tcccaggccg ccaggtcgtc cagatcccgg gatgggggaa aaagggcggc
    56821 gggaaggagc ctccgccatc cccggggggg atggatttgg gcgtagagcc tccgggcctc
    56881 cagggtggag ttttcttccc ccaccagggt gacggcccaa ccatatccct ccatgagcgc
    56941 caggaagggg tgtcgcgccg tgccgtctcc caacacaaag tgctccggaa aagaccgcac
    57001 cgcctctgca tgcccctcgg tgctccaggg caagagggcg tcccatcgtc cctcccgtac
    57061 ccgggccccc caggcgtccc tcgccgccgt gggggtgatg cccgcaaaaa gtagggtgcc
    57121 ctcctcggag ccgaaggccc acccgaactt gcccctcccc ggatccactc ccaggatgat
    57181 catggcaagg cctgggtgga ggcccaccgg ggatagagcc acatccaatg atcggggtgg
    57241 cgccggatcc atgtccccaa cacctcgttg cagagggcca cggaatactc ttcgtcctgg
    57301 ccaaagggga ggccgtcttt gccctccagg gcagggagaa ggtgaaggtc gtgggagatc
    57361 ccgtccgggc gccgcaccat gaagaggggc accacccggg atcccatttt agccgccatt
    57421 ttcaccgggc cgtagggcgt actggcggga tgtcccaaaa agggaacccg aatcccggcg
    57481 ctacgggcgt cctggtccag gagaaccccc aggatctccc cccggcgaag gcaccgtagc
    57541 gctcccttga ggtcgaagcc cttgccgatg gtccgcaccc ccgccgcctc ccgcaaggat
    57601 atgatgagat cggtaatacg gggatcccgc tgctccgccc ctatggcgtt catggggaaa
    57661 ccccgggccg ccagcgtggc ggcggccagc tcccagctgc caaagtgggc cgtcaggagc
    57721 accaccccgc gccccccttc cagggctctc cggaggtact cctctccgtg caccgccacg
    57781 cgggatcgcc accgctctcc tcttttgggg atgagcagaa actcgaccac gctccgcccc
    57841 aggttgacgt aggaggcccg cacgatccgt cgggcccggg tcacccccac tcccagggcc
    57901 cccacgcacc gggcttccgc cccgtccacc cgccccttgt ccagggccca gaccaatcct
    57961 cccaggactc cccctccaaa gagggcccaa tccggaggca aggcctcgaa ggcccttcgt
    58021 accacgccca gcgcacgcca cgtcacatcg cttcccatgg gagacctcct accccagcgc
    58081 cttgacggcg cgcccttcgc gctctatgct gatggagaga ttttagcgtg ccatcgcgag
    58141 aaggaggaat ccccatgctg cgaagaatac tccaccccat cgatctctcc ccccagtccc
    58201 tggaaggggc gaagtggatc ctggactccc tggccactcc ccggacctcc ctgcttttgg
    58261 ttcacgtggt caaccccgaa gcggggctgg aaactcctgg cctcgtacgg gacgccgagg
    58321 agcacatggg tcccctggaa gaggagctcc aggccctcca cggcggcgtc agctccctgg
    58381 ttctcgcagg agacgacgaa gaagtcctgg cggaggtggc tcacagagaa aagtgctccc
    58441 tggtggccca tatcatccac cccgaagacg accccctgcc cctgatacgc cccatccccc
    58501 tgccccatct cttgattccc ctccccgacg acggcacgct ccccgccggg gatcttttct
    58561 ctcgtatggt ggtcgccacc gacctcgccc ccgatcgcac ggacgcccta ctggaatccc
    58621 tccggcgcgt ggccgagggg cgggctcgcc agaccgtgct cttgcacgcc gtgcccctgg
    58681 acgaccccgg ggcggcggaa gagctcgtca tggcggcgga cgaggcgctc caagcggtga
    58741 aagaatctct cctcccctgg ggaggggaag tgaccgcccg cctggtgagc ggagacccga
    58801 acgaggaact tcccgccgtg ctggaggacc tcgccccctc tctgttagtg ctagggctat
    58861 cccgccacgg cgaaatgtgg gggctcctgg tgggaagtac cgccgaagcc cttatcggcg
    58921 cggcaaaaag tcccgtcctg atcatccccg cctgaggctc cattataggg catggggcgc
    58981 ggacaccggg gggggattcc atcgcctgag aaaacttttt cccgtcacac gacgtcgact
    59041 ttcgatggtc gcgccgcagg gcgccacggg actcccgggg agaggcgttt cctctggggg
    59101 gggttgtttg cggggggtga cacgatgcat cgtagccgtc gcggagtttc tctcaagttt
    59161 cagatcttat ccggcgtggc cctcatcgtc ctgggggtct tggctgcgtc cttttggggg
    59221 agctacctca ccgcccgaga taggctgatg gagagcttcg tggcccaaga atcgggggcc
    59281 gtgtccggtc tggcccaggc cacggacaag accctggagc tcctgaacca ggcctgcgtc
    59341 tctaacctgg acgtggccgc cagcaccctg acccagggaa acatcatcgc ccgccccccc
    59401 ggggccgtgg gaacatggca gcttcctcag ctgctccttt cctgcggagg caaggtaacc
    59461 aagctcaccg gaaacaacga cctccccgaa gagtggagtc ggcgcatgcg aggggtcttc
    59521 agcctctttc aggtggcgtc gtcggacggg aaaagcgccc tggtgcgcgt ggccacgtcc
    59581 ctgcgcgatg gccagggaaa ctccatggtg ggcacggtga tccccgaaga tagcccggtt
    59641 tttcaaagcg tggtgaccga aggaaaggaa ttcccgggaa ccaccacgat ccaaggagag
    59701 ccgtacttcg ctagctaccg ccccctcccc ggccgcgatc cccaggattt tatcgtgata
    59761 tcatcgggca cttcacttca gcccttggtg aagctcatcc gggagaccca tatcggcgaa
    59821 gggagccaag ccctgatcct cgacggggag ggaagacctc tgggaggggc cgacccggga
    59881 gccgccgagg aagaaattgg cgccgtcctg agagaaaacg gccgggggca gaccccgggg
    59941 acggtctgga gtttcgcagt gggctcgggc agcgaggccc gccaggtctt tgcctttcgc
    60001 actgaagacc cctcttggac cgtggccctc tccctccctt catcggtgat cctagccccc
    60061 ctgggggcca tgagggggcg catgatcttc tggggtctcc tatccctggt ggtgggcatg
    60121 gccttggcgg ccatcgtcct tcgccgcctt ctcgcccccc ttcgttcggt gatagatacg
    60181 gcggagcgag tggcccaggg agacctgagg gcttgctccc tggggcaaga agaaagccgc
    60241 aacgagatcc aggtggtcct gggggccttt tcccgcatca gcgcctcctt tcgggatctg
    60301 gtgctccgca tgcgccgcat ccaggagcta tctcaagagc ggggcgacgc catggagcag
    60361 atcaacgtgg aagtggccga ttccctccga gaggcgctgg cggcggcggg agatatggtc
    60421 cgcaccgtgg gggacgtggc ccaggcggca gagagcacca aggcccgagt ggcctcggtg
    60481 acccgatcct cccgggaagc ggcggagctg gtccaagacc tatcccgaaa ggccgaaacg
    60541 gtggcccggg aggcggcctc cgacggcgac gcggtgcgct ccgtggcggc ggagatcgcg
    60601 gaggcgggaa aggccagcac ccgtgtcagg aatgccctgg gagccctgga gcactcggtg
    60661 gaaagcatat ctcgcttcgt ctccaccatc cggggcatcg cggaccagac gaacctcctg
    60721 gccctcaacg ccgccatcga agccgcccgg gccggagaag ccggtagggg ttttgccgtg
    60781 gtggccgaag aggtgcgtaa gctggcagag gagtcgaacg aggcggcgcg ccaaatccag
    60841 tcggtaattg aagaagtggg cacccggacg gacgagaccg tgcgggacac cgacggcgcc
    60901 attgagctca tatccaaaac ggtggagaac tccacggggg cggccgaacg tatcggaaaa
    60961 atcatggaag acgtctcgga tatcgccaag ggcatccagt ccatcgccca ggccacccag
    61021 gggcagctcg ctggcaacga ggccgtggaa ggcgccatgg actccgtaca cctccagatg
    61081 agcgaaggac accgggccgc ccatagggtg gaggaggggt ctcgccaggt ggccgcccag
    61141 atggagcgcc tggaagaaat acggcagggg caacaggagg tgctggacga tctggaagag
    61201 attctccagg gctacgtcct cgacgaaggc ccgtgacgac cgaaaacctg aggctcgcgc
    61261 cccttctttg ccccctaggg ggcaaagaag gggcgcgagc cttcccaaac ccccgagggt
    61321 tacctgccta cggctctccc aggagacgta gcatgacgga gtcggcgctg gagaatccca
    61381 gggtgctcag ggtccccacc gcccgggcgg tgtcggccaa ggtggcatag accactcctt
    61441 ggcgctcccc caccccgcag ttttccaggg ccagcagggc actggtgtag gcctctccgg
    61501 cggcggcccc caccttgagg gcgcaggacc ccttggcccc atcgcacaac attcccacca
    61561 aagacgccac cagagaagcc acggcccgat ccccctgggc cgccgtcccc cccgccaggc
    61621 gcaccaatcc cgccgccgcc ccggcccccg ccgccacggc gcacccgcag atgggggtga
    61681 gccgtcccgt atgggccttg atgtatcccg tcaccagatg actcagcgcc agggcctccg
    61741 ccagggatcg gaaaggacta ccccaatggc gggccgccac caccaggggg atgatggcgg
    61801 tgatgccatg atttccgctt cccgcgctgc tcatgaccgc caggggaacc cctcccatcc
    61861 gggcatccgc tgcggcagag gcggtggcct tgatctccca gagaagatcc ccgggggtgc
    61921 cgcgctcctg taggacccgc cccactccca acccccaatc accctggagg cccgcctctc
    61981 ccacttccat gttcgtccgg gccccatcca agagaaagat ctccatttcg ccatccaggg
    62041 ttccggcgag gcgccacaga tcttccatgg tcaggcgcat gagctcttcc aggtacgcgg
    62101 gcctaccctg gaccgaggtg tccgcctcca acaagcggcg caccaccttg ccgtttttcc
    62161 tcacctctgc cacccggtcg tgggatccgg aaaccacggc ccccgccgtg ccttcggggc
    62221 cccggagctc cacctcggca tagacgctgg gcacgtcggg caccacttgt tccgtgacgt
    62281 tccctgtttc caccagcttc tgggcccgct ccacggcctc cgagggcagc ccttccaggg
    62341 atctaagccc cttggatgcg tcccccgcca gggcccccag ggccgccgcc aggcgattac
    62401 cctggagccc ccccgtaccg gggatgccca cgctatggcc atttttgaac acattcgccg
    62461 agaggcgaag cctaatgccc tccaaggggc ccgaaaactc cttggccgcc gtggccgccg
    62521 ccagggccac cgcccccggc tcggtacatc ccaaggcggg cttcacttca gcccgcagaa
    62581 aagccctcag atccatgccc atcgcccccg tcagtaattt taatcttttt tgccctcgtc
    62641 ccgaagcccc gcgcgccttt tgtccaggcg ccacgccgcc cccgccacca aaaatcctcc
    62701 ccccaggccc aagatcccca cccccgccag ggtgcccccc atgccccaac gcgttcccaa
    62761 cagggcggcc cccacggtgg taactatcca ggccagatcc atgacgaaaa atgatcccgc
    62821 cacacccttg ggcagaaggt gccgggggac aaagtccggg gccagggcga gctggatggg
    62881 aaagccaaat cccatgccca cgccgtacac aagccccaag atccccagaa aaagatgggt
    62941 atctccccgg ggaaagagga ccagcactcc ccccatgagg gcgatcatga cccctgacag
    63001 gatcccccgg ggaaaacggt ccagcacccg ggatcccaac aggcgcatgg ccaacgccga
    63061 ggcggcgttg gccacgaaaa acgcgctgga gctcaggcct ttttccgcag cggcggcgct
    63121 gaggtactgg agggtggagc tattcaccag ggaaaacacg gccccggagg ccaacacaaa
    63181 gtaaaagggc ctcacccgaa ggagctctcc ccaggaaccc cagccctgat ccggggtcgc
    63241 cgtcgccggg gggaggtccg acgaaaaaag gctcgccccg aggcatccca aagccagcac
    63301 cggagccagc gccagataaa gctccgtgcc cccccgggac aggaaaaagt cccccagggg
    63361 catcaccgtg atctggggga ggacgtaagc cacggcgatc caggcgaaga cttgcccccg
    63421 aacggcggcg ggaatggccg ccgtctgata cgccgtcacc gccaccatgg ccaccccgta
    63481 gaagagcccc atccctcccc tcaggagcaa aaggggcccc agggaacgca cccagatcat
    63541 gggcgtggtg gtgagcacca atcccactcc tgagaggatc agggcccacc gcacccccag
    63601 gcgctccacc atcacccctc ccagggggcg ccccagggtg gtggcgccga aataggcccc
    63661 caaaagcccc cccaccagcg tcagggggat cccttcctgc tgcaaggcca cggggtacag
    63721 aaaaaacacc gccacatagg cctgcaccaa gtaggtggcc aaggcggaca accccaggtc
    63781 acgacgcttc atggctcccc catccctccg gagcgccgga ggatcccatc atcatcgata
    63841 cgttcccaaa ggcggtggga acctaccggc acacgccctc ttcccgggcc gcccgggcca
    63901 ccgccgccgc cacggagggc accacctccg gatcgaaggc cgaggggatg atgtactccc
    63961 gggtgagccc atcccccacc atctccgcca gggcccttgc cgccgcccgc ttcatgccct
    64021 cggtgatgcg gagggccccc gcgtccagag ccccccggaa aataccggga aatcccaggc
    64081 agttattcac ctggttggga aaatcgctgc gccccgtgga gacgatggcc gctccccccg
    64141 cccgggcctc ctcggggaaa atctccggca cggggttggc cagggcgaag accgcgctcc
    64201 ccggcgccat ggaggccacc atctcccggg tcaccgtccc ccctttggag acgccgatga
    64261 agacgtcggc gccccgcagg gcctgggcaa gatccccccg cacgccttcg gggttggtct
    64321 cccgggccag gagacgctgc accggagagg cctggggatc ttcgggatcc aaaacgcccc
    64381 ggcggccgca gcacaccaga tgccgagccc ccgccgaggc caacatgcgg gcgatggcgg
    64441 tgccggcggc ccccatcccc gatatgacga tccgggccgt ctccagaacc ttgccctcaa
    64501 gtttcagggc gttgagcact ccggcgagga cgatgacggc ggtgccgtgc tggtcgtcgt
    64561 gaaacaccgg gatggaaacg cgatcctgaa gccgggcttc gacgtcaaag caccgggggg
    64621 cggagatgtc ttccaggttt acgcctccga aggatggggc cagacgcgcc accagatcca
    64681 ccagggcgtc ggggtcccgc tccgccacca ccaggggaaa ggcgtcgatc cccgcgaagc
    64741 gcttgaaaag aacgcacttg ccctccatca ccggtagggc ggcctcgggg ccgatgtctc
    64801 ccagccccag aacggcgctg ccgtcgctca ccaccgccac gcagttgccc cggttggtga
    64861 gctcccagac ctgcgccacg tttttttcga tggcccggca gggctccgcc actccggggg
    64921 tgtacgccag agatagatca tgaaagttat ccacgggcat ccgggactcc acggagatct
    64981 tacctcggtg cctccgatgc gcctccaggg cttcccggta aatatcgtcg cgactttctc
    65041 ccaccgccgc atcctccttc gttcgtctcc ggggagaggt cctccccgaa ttcttaaggg
    65101 cctcaggccc cggtcccgct tcctttcagg ctttcaagaa acgccgccgg gccctcatcg
    65161 tagaggtcgt gtccccggag gtctaccacc accgtcacgg gaaagtcctc cacctccagc
    65221 gccgtgatgg cctcggggcc caggtcgtca taggccacgg gaaggactcg ccgcaccgac
    65281 cgggccaaga gggccgccgc tccccccgtg gcgccgaagt ataccgcccc aaagcttacc
    65341 atggcgcgca ccacctcggc gctccgccgc cccttgccga tcatcccccg gagccccagc
    65401 tccaggagca ggggcgtgta gggatccatg cggccgctgg tggtgggacc aatggctccc
    65461 accgccctcc ccgggggggt gggagagggg cctgcgtagt agatcacctg cccccggagg
    65521 tctaagggaa ggggctctcc ccggaggtgg acctccacca tgcgccggtg ggccgcgtcc
    65581 cgggcggtga agatcgtccc cgaaaggagc actgcgtcgc cacaccggag gcctcggagg
    65641 gcctctggaa ccaggggcgt cgtcatcctg aagggggtct ccggtcggga tatcatgatc
    65701 ccctctcctc tcccccaagc tctcccgagg cgtggcgatg ggcgtgacaa catatgttta
    65761 ccgccgtggg caagcccgcc agatgggtgg gaagccactc cacgtgtacc gccagcgccg
    65821 tgatgctccc tccatatccg gcgggaccca cccctagggc gttgatctct tcgagtatct
    65881 tccgttccaa cgcggcatag cggggatcgg ggtggggatc gccgcagggg cgcaacagag
    65941 ccctcttggc cgcccggggg gcggtttcga agtttcc
//
LOCUS       DAOOER010000005        64697 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_475_length_64697_cov_8.673154, whole genome shotgun sequence.
ACCESSION   DAOOER010000005 DAOOER010000000
VERSION     DAOOER010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 64697)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 64697)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64697
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_475_length_64697_cov_8.673154"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PL162_01205"
     CDS             complement(<1..542)
                     /locus_tag="PL162_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQF90678.1"
                     /translation="MAKILLQMKEITKTFPGVRALKGVSLDVREGEIHALVGENGAGK
                     STLMNVLSGVYPAGSYEGEILFDDRPCAFHHIRDSEEVGIVIIHQELALVPYLSIAEN
                     VFLGNERARRGIIDWEETFSRTRELLLRVRLQENPATITKDLGVGKQQLVEIAKALSK
                     KVRLLILDEPTAALNDEDSE"
     gene            complement(676..1728)
                     /gene="chvE"
                     /locus_tag="PL162_01210"
     CDS             complement(676..1728)
                     /gene="chvE"
                     /locus_tag="PL162_01210"
                     /inference="COORDINATES: protein motif:HMM:NF040907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HQF90679.1"
                     /translation="MKKAIFILLTLVLALSATAALAADVLVGVAMPTQSLQRWNEDGA
                     NMKKALEEKGYIVDLQYAEDDVSNQIAQLENMITKGCKVLVIASIDGSALSDVLAKAA
                     DAKIPVIAYDRLIRQTPNVSYYATFDNFKVGVIQGKFIEEKLGLTEGKGPFNIEFFGG
                     SPDDNNAFFFNGGAMSVLQPYLDKGQLVCQSGQTKMEQIGILGWKSEDAQARMDNLIS
                     KSYTDKKLDVVLSPNDSLAYGIAASLANNGYTPGKDWPIITGQDCDRANVKNMIAGKQ
                     SMSVFKDTRALAAKVAEMVDSIVQGKDPEVNDTKTYDNGVKVVPSYLLEPVFADINNY
                     KTLLLDSGYYKEADLQ"
     gene            complement(1938..3257)
                     /gene="xylA"
                     /locus_tag="PL162_01215"
     CDS             complement(1938..3257)
                     /gene="xylA"
                     /locus_tag="PL162_01215"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF003998.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HQF90680.1"
                     /translation="MEDLFPDVGPVRFEGPGSKNPLAFRFYDAEETFGDKTMKEHLRF
                     ALSWWHTVTAEGADMFGGGTWQRPWDGIADPLLHSRRRAEGAFEMMTKLGINFFCFHD
                     RDLVPEGSSLRESNQRLDAMVEVLRGLMKATGIRLLWNTSNLFSHPRYVHGAATSCNP
                     EVFAFAAAATKKSLEIARELGAENFVFWGGREGYETLLNTDLALEQDNMARFFHLALD
                     YARQIGFKGQFLIEPKPKEPTKHQYDFDVATVLAFLRKYDLLPYFKINIEANHATLAG
                     HSFEHELALARVHGVLGSIDANEGDLLLGWDTDQFPTDVRAATLAMMQVLAAGGLAPG
                     GLNFDARVRRGSFEPRDLFYGYIAGMDTFARGFRAAWSLRASGELEDFVARRYAGWHE
                     GLGARIRQGKEDLASLEAYALEHDHIQNASGRQEMLESVLNRHLYPI"
     gene            complement(3323..4516)
                     /locus_tag="PL162_01220"
     CDS             complement(3323..4516)
                     /locus_tag="PL162_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HQF90681.1"
                     /translation="MTAFNAERMGHNNRLTLLNMIRHHPGISRRELALSSGLDPSTVT
                     KAVSLFLDQGLVEETGEKKSPGAGRRSVSLALRRAGGVAVAVKVGVERTALARGYLDN
                     SYEIWAEFPTPSDPEEFFDTFAREVRCIQGVLPPDQRLVAFSFALPGMVDAETGMGVS
                     FPHLGWHHIDFSQEILRRLPDHRALLLLANEAQLSLTAEARRHPELAAAEGGIYVYLA
                     HGVGGGIMLRGSVHRGASNCAGELGHMGVDPTGPLCHCGHRGCFETFVSINPVVRDYE
                     ARASTPLRGPENSQKYREILRRREEGDEVAREVLCQMSGHLVRGLRNLCNILNPEFVM
                     LGGVGADYPDSLLEEIQETLRRQVLELAAKKLRVVRASMDIDASALLGATMVAMDCHA
                     RTTVG"
     gene            complement(4712..5365)
                     /locus_tag="PL162_01225"
     CDS             complement(4712..5365)
                     /locus_tag="PL162_01225"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQF90682.1"
                     /translation="MKRILLARHGVTSWNQERRFQGCSDIPLTDEGRHQASRLATRVA
                     LWKPHRIVTSPLIRARDTAEAVRRACGGHPPCEVSEGLREMSFGAWEGLPVEQLVQDA
                     GESLNLWARDPGKFTPPGGEPIACTQQRALEALNPILAGGDPEERILLVAHGGVISAL
                     LLALFSMPTQMFRGILPGNCALSAIHLDPDQRFLVFLNDVLHMAVAEELVGQLPIPA"
     gene            complement(5560..6510)
                     /locus_tag="PL162_01230"
     CDS             complement(5560..6510)
                     /locus_tag="PL162_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90683.1"
                     /translation="MDRKGLLLVFYAVGAILTAAGPGQGASPTAARMVLLHHSTGEVI
                     WYGGVPEWFDEYNRTHGTSHRISEQHFPEDGDNSPFDYWEIWVQHGAEAQFRGSPTLL
                     TLTAQYDVVIWKHCYPVSEIEADTGNPDVTSSTRTQENYKLQYLALRDAMRAYPQTLF
                     VVWTGAVHTRANLAEEQALRMRAFVQWLRTTWDEPGDNIVLWDFYQLETDGELYLKDA
                     YATSPTDSHPNEAFARRVAPNLCQTIVNALTAGPSPTAGPSPTAGPSPTASPSPTTGP
                     RPSTTPPRTSGGGGGGCAIGAPGWSILLGLLPGIFLFRRK"
     gene            6732..7334
                     /locus_tag="PL162_01235"
     CDS             6732..7334
                     /locus_tag="PL162_01235"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQF90684.1"
                     /translation="MYHAVIRAASSARRWIFPDVPQGGLLHLSDTPRGTFCFIKRLIG
                     RTRPAVIIHTGDLVDDIKLELRPRERDAYAQQLARLLPILESAGASVHVMLGNHDDEE
                     AVRNLGGGVVVHPREVALSWAGKVLRGAHRAEDALEGPASFYLFGHDLSVKSSPPDDP
                     VQRLNGLEHVHLISPAGEVLRLRWPWGTEDARLLRHRCGI"
     gene            complement(7404..7910)
                     /locus_tag="PL162_01240"
     CDS             complement(7404..7910)
                     /locus_tag="PL162_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90685.1"
                     /translation="MRKVSCGVVAMALIVSLYGSEAWGEDPLERFNTVMEKTIVAFGN
                     SVEEVTRRLGAPTGEEISPDVSPHDPSAHFEWVHLTYPRRHIALFRAPDKEFLVLVDT
                     QQEGDIFGENIRVGSSRQDVVRELGEPLEMRDRAMIYEDEAGYTELAFFLNAQGVVER
                     MMLSIMLD"
     gene            complement(7991..8527)
                     /locus_tag="PL162_01245"
     CDS             complement(7991..8527)
                     /locus_tag="PL162_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQF90686.1"
                     /translation="MAFLDLAHLRRSVRRYRPEAIPREALERCLEAARLAPSACNSQP
                     WRFVVVESPELRQSIVREALSGPYAMNSFAASAPVLVVAVTEKSRWAARMAGIFRGVP
                     YALLDLGMACEHLALQAAEEGLGTCMLGWFAERAVKRLLTLSPLERVDLLLTLGYPDD
                     GPPRPKIRKPLEELRRYV"
     gene            complement(8541..9914)
                     /locus_tag="PL162_01250"
     CDS             complement(8541..9914)
                     /locus_tag="PL162_01250"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQF90687.1"
                     /translation="MEGVTGSAAFTLWDMAAFMVALAVLFLALDGIYQIAVSIFGWGR
                     FTDSPRAQGRLNFFVLVPAHNEARVIGPLLDSLQNQDYPRERFEVCVSCDNCQDATAE
                     VASRYGVRVLVRTTEVRSGKTENVRWALEQVPLEHSDAIVVIDADNLAERDFLSRMND
                     YLEAHPDADAVQGLLDVKNPRDNWLARSYAMAYWYTNRFWQRARGNLTLSATLGGTGL
                     AVRTPLFLQMLAFVGSLTEDLEMATMVVLSGKRVHWNEHAVIYDEKPTDLGVSFSQRV
                     RWMQGHFWVFGHYGPTVLRRFFSTGRLRYLDLFLYLAAPAKATLSFLCLVGCALLVTF
                     RPEATWAHPWVQPIAHLGLGWGALPISLGMVFVYGFFVAVVGPSLRHRRLDLSYFFYA
                     PAYLWFGLTWLPVLFKALFLAYDQGTWVKTEHTRHIVLGDLARLAGRSPASPALAPKE
                     EPLRVRP"
     gene            10188..10388
                     /locus_tag="PL162_01255"
     CDS             10188..10388
                     /locus_tag="PL162_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90688.1"
                     /translation="MTDKNKRSVWHGERQGKPVLVFLAAVVVALGIAYASVSLLSDTG
                     WDNLEIVRMFQKHFMAREGAGK"
     gene            complement(10525..12609)
                     /gene="fusA"
                     /locus_tag="PL162_01260"
     CDS             complement(10525..12609)
                     /gene="fusA"
                     /locus_tag="PL162_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQF90689.1"
                     /translation="MAQKPEDIRTVVIAGHGGAGKTTLAEAVLFDAGVTTRLGKIEDG
                     NTTLDFSLEEQKRQISINAALATVPHKGKTFYLLDSPGYADFTGEMRSCMYVADGVVL
                     VASAVHGVEVQTENAWESAAEHHLPVAFYLSKMGRENADFFRIVGELQKYLSEKATPL
                     YLPIGAEAAFKGVVDLLHEKAYLYKGDGSKGGKEGPVPDDMTEAVKAARDALVERIVE
                     ADDELMMRYLDGEQVGTDELIPALRKAVRDRLIFPVIPGESGGNVGVEQLMDLLAEYF
                     PAPMERAPRIALEGETETPLIPDPKGPFVAFCFKVMVDPYVGKLSFLKVLSGALSSDQ
                     NIFNVSKGEDERISAFKLMTGKEGKDAKEIVVGEIVAIPKLHSAAVGDTMGTKGCKLI
                     VPPLEFPQPVYSVAVTPKSRADEDKLATAVHKMLEEDPSLSFRKDPETNDNVLSGMGD
                     VHLDIMLARTRERYGVELEVATPRVPYREAIKKPSKAQGKYKKQTGGRGQYGDVHIEF
                     SPRERGAGFEFLDKVVGGVVPKSFIPAVEKGLREALPKGYLAGYPMVDISAALVFGSY
                     HDVDSSEMAFKIAASLSFKEAMKNASPVLLEPIMNVEVMVPEEYLGDVMGDFNSRRGR
                     IMGIDAKGRMQVVRAQVPLAEMFRYAIILRSMTSGRGFFSMEHSHYEEVPGDISKKVI
                     EQRQSEVQSVEE"
     gene            13440..14489
                     /gene="ord"
                     /locus_tag="PL162_01265"
     CDS             13440..14489
                     /gene="ord"
                     /locus_tag="PL162_01265"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HQF90690.1"
                     /translation="MRKENVKVIIWGLGAMGKGMAKMMLEKKGFEIVGVVGRGAKLGK
                     SMYEFLDMPRGSRPDVVIGTPEDVIKPGAADVVLLCTDSFVKSAFDKIKFAVERKINV
                     ISSAEEMAYPKAQEPELAKEINALAKAHGVTVLGTGINPGLMMDLLVVVFTGACETVE
                     HITSRRVNSLSPFGPAVMEEQGIGLTVEEFKKKRDEGHLAGHVGFAESIHMMADAIGW
                     KVDKVTQSMEPIVTEVDRKSPYGFAKAGDVAGCAMKGFGYVNGELKIEMDHPQQIEPH
                     LGGTDTGDYVIIKGTPNVNMANKPEIPGGIGTIAMCVNMIPHVINARPGLKTMLDLPV
                     PRAIMGDVREMIEEE"
     gene            14683..14991
                     /gene="ortA"
                     /locus_tag="PL162_01270"
     CDS             14683..14991
                     /gene="ortA"
                     /locus_tag="PL162_01270"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HQF90691.1"
                     /translation="MQGAKKGDWVQIRQVVLPPEGRAPQVPEDTRGTPLLLWVKGFAQ
                     SDAAVGETVEIETMTGRRVKGELCGANPRYIHDFGNFVPEFLQVDLQLKALMEGEISR
                     "
     gene            14988..16412
                     /gene="ortB"
                     /locus_tag="PL162_01275"
     CDS             14988..16412
                     /gene="ortB"
                     /locus_tag="PL162_01275"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtB"
                     /protein_id="HQF90692.1"
                     /translation="MTGRDMSYAAVMGRKNEIMKKAIGIDYQTFESGGLAFDYERMMR
                     EASYSLEEIQQIQYSTGVGNTPILELANLTALARKFAPAGKGARIFIKDEACNPSGSF
                     KARRAANAVYHAKKHGYKGVVTATSGNYGAAVASQCAMRGLKCIVVQECYDSRMIGQP
                     EILEKQRACEAYGAEVVQLTVGPELFYTFLSLLEETGYFNASLYTPFGIAGVETLGYE
                     LVTQFREKLGRAPEKVVITHAGGGNVTGTARGLLKAGATGTEVVGASVDLSGLHMASD
                     KDFNRKSFTTGHTGFGIPFATWPDRADVPRNAARPLRYLDRYLLVKQGEVFYITEALA
                     VLEGLERGPAGNTSLTAAFALAQTMDRDHFIVVQETEYTGAGKHPLPQISFAKQNGIE
                     ILFGDPDTEVPGKNIVFPDSPERIRLRDFDLAKARTSYIRNSVTQAKRESISKEDLAF
                     LAAETKSDENFVLGVLKELNVSVK"
     gene            16472..16822
                     /locus_tag="PL162_01280"
     CDS             16472..16822
                     /locus_tag="PL162_01280"
                     /inference="COORDINATES: protein motif:HMM:NF027866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HQF90693.1"
                     /translation="MKRADDFASRRQHLAGLSDEELKKRFWELAEKIVEPMLELGRTH
                     TTPAVERSVLLRMGFSSLEVKPIVEGVIAKGLMGKGAGNVVWRLSKKLGISVREAGVA
                     LSQGQHWDVVDTLF"
     gene            16841..19036
                     /gene="oraE"
                     /locus_tag="PL162_01285"
     CDS             16841..19036
                     /gene="oraE"
                     /locus_tag="PL162_01285"
                     /EC_number="5.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine 4,5-aminomutase subunit OraE"
                     /protein_id="HQF90694.1"
                     /translation="MKLQPNKRLDIKELLKDLENYRPKRRGWHWREGRNEPRIVGDFE
                     YHEASEGLTNYVPLPGSRGFHYIDPQPDCVITTEIASGRFEDDVRRMRMAAWHGADHI
                     MVIRTAGQSHIDSLLEGTTQGIGGIAVTRKQVRASRKALDAIEEEVGRPINFHSYISG
                     VAGPDIAVMFAEEGVNGAHQDPQYNVLYRNVNMVRSFVDACEAKKIMAWAGILQIDGA
                     HNANATAMKAWKVRPELMVQHAINSIFSRKVGIKPENIALSTVPPTAPPAPCMRLDLP
                     YAVALRDFFSEYKMRAQQNTKYMESDMREATVTHTLNLVISRLTSADVQSTITPDEGR
                     NVPWHYNNIAAISTAKQALNGLDGLREMVKLDREGPLGVEVRELKERAVLFMEEMLEV
                     GGYFKAVEQGFFIDSGYYPERNGDGIPRQADGGIGVGMLFERDKDYFAPVSVHYGYNN
                     IPAEFGGKASAAIGGDTFEDPSKIVFIDELDENDNVDARLREQSRYYETEFIKPEVEW
                     RADGVVALNLFLPVDERTAEFAAIRCGEKMGLEDVTVVHKQAMHPSEGTYVELKGRVN
                     FDINVNDLVIPEKIPVLSEEEIREDIAANPLFVVAATVGQDEHSVGLKETLDIKHGGV
                     EKFGIKYLYLGTSCPVEKLVDAAIETNAGAILASTIITHDDVHVRNMRKIHELCVEKG
                     IRDKVTLICGGTQVSHEIAREAGMDAGFGRGTKGIDVASFMVKFRRGQA"
     gene            19041..20405
                     /locus_tag="PL162_01290"
     CDS             19041..20405
                     /locus_tag="PL162_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmL-related ornithine degradation protein"
                     /protein_id="HQF90695.1"
                     /translation="MKIAVLVAEIGSTTTVVNAFDGIGGPCPRFLGQGQAFTSVLGGD
                     VTVGLRGAVEDLRRHLGVSEISWGEMFATSSAAGGLRMSVHGLVYDMTVRAAKEAALG
                     AGGILKHVTAGKMRRTDLKKIVEIRPNIVLVAGGVDYGERETALHNFELVADLGLDIP
                     VIYAGNVENHDEVREIAEEKNLRLYLVENVYPRVDQLNVEPTRAVIQQVFEEHIIHAP
                     GMGTVRDMVDGPILPTPGAVMEAAKVLKEAIGDLVVFDVGGATTDVHSVTEGSEEIGR
                     LMVSPEPVAKRTVEGDLGVYVNMYHIVGKIGREELLREFPDLDSLLAGARAIPDTDRS
                     KAFVERLTETAMLTALERHAGTLRQLYIATGKRTIAEGKDLTAVRFIVGTGGALTRLP
                     RHREILARAAGYGKGMELFPKAEARVLVDNDYIMASLGALSRRYPDDARKLLLNSLHW
                     EEGA"
     gene            20595..21656
                     /gene="orr"
                     /locus_tag="PL162_01295"
     CDS             20595..21656
                     /gene="orr"
                     /locus_tag="PL162_01295"
                     /EC_number="5.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine racemase Orr"
                     /protein_id="HQF90696.1"
                     /translation="MPYPKLVIDTKKLAHNTRVLAERCAARGVALAAVTKVYCAFPEL
                     ARVQAASGVAWLADSRLENLAKIQDIPVPKILLRLPMISQAEEVVALADLSFNSEIAT
                     CKALAAAAQKQGKIHDVLLMVDLGDLREGVWPDRAVEAAGEILALEGLRLRGVGVNLT
                     CYGGVLPSVENMTRLVEVAEAIEARYGLSLDIISGGNSSSLKLLASGKMPSRINHLRL
                     GESVVLGFETAEGTRIPGTEGDAFTLAAEIVELQTKPSVPVGEIGRDAFGQVPVFEDR
                     GMRRRAIVAAGRQDLRIDAMSPRDDKIIILGASSDHMILDVTDSSREWQVGDAVEFDL
                     AYGALLAATTSPYVTKEFI"
     gene            21736..21810
                     /locus_tag="PL162_01300"
     tRNA            21736..21810
                     /locus_tag="PL162_01300"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21768..21770,aa:Ala,seq:ggc)
     gene            21975..22907
                     /locus_tag="PL162_01305"
     CDS             21975..22907
                     /locus_tag="PL162_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HQF90697.1"
                     /translation="MRFENRKRKASSSLGASVDPRSLQDYLEYRKGSGLSKATLGLDR
                     AILVPFAREFPAWHEDIRAAMVGYLDMSRKAWTRSTRLRVFKGFIKFLVEEGELPENV
                     DPLRGVTAPLPGKTATAATPEGVSAFLAALGASWEHKRLAVLVQLLADTGLRKNEACS
                     LRWADYDSAARRVVVRAENAKTRRERTVPVSGDMVRVLRKYRDNLPARLQESEWIFPL
                     SQKDHIPAAAVGRQVNRISHATGVPLRLHSIRHMAATAIIRATGNIALAAQLLGHSQI
                     STTAGFYTHLGLDDVRAAQEQVGTLRSLRAARHV"
     gene            22900..23397
                     /locus_tag="PL162_01310"
     CDS             22900..23397
                     /locus_tag="PL162_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90698.1"
                     /translation="MFSPVTLFCLPAEERYFVLQRSLPQCLDIHRAEGVVWGSGVPEL
                     GESRTRFLRRTMSTEQRQAFEELFPEGVVVNVVVVAGDDPRNARKFSTVSLSGGHRPD
                     ALPLTLEVEGVPEPGEGLSFFVHRCMTAEQRAMLEEYFRDPSGASVCTWEVASISLDA
                     VTVRV"
     gene            complement(23680..24054)
                     /locus_tag="PL162_01315"
     CDS             complement(23680..24054)
                     /locus_tag="PL162_01315"
                     /inference="COORDINATES: protein motif:HMM:NF015550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051276 - chromosome organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HQF90699.1"
                     /translation="MLNYRQRAFVEAYCGDAQAAAIAAGYSPKTAKVVGCRMLKNPEI
                     VGLIEAREEARASAAVATREERQAFWTAVMRDAEEPMKNRLRASELLGRSCGDFLERV
                     QVSTTEVPTIHVIFGDPVEEAE"
     gene            complement(24755..25078)
                     /locus_tag="PL162_01320"
     CDS             complement(24755..25078)
                     /locus_tag="PL162_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90700.1"
                     /translation="MTALRTARKLLERGLVPLLQEGELRVLPLCVVSKAAVHLTDTER
                     QALRQALEDPAQLLAELEGTLTPVERELYEERRGILEADGIDAPWLCFADTVRTYARW
                     RRSSV"
     gene            complement(25075..25728)
                     /locus_tag="PL162_01325"
     CDS             complement(25075..25728)
                     /locus_tag="PL162_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90701.1"
                     /translation="MLDVCRPVILNGIGGSWLDRPDLLSRSIVVQLEPIEEQDRKPAA
                     EIEARFRAVLPKIQGALLDAVSDALRHSHDAPSDSLERMADAHKFVLAAELGGRLPWR
                     CGEYTQALTQGRRVAELDALEDDGVAVRLRDLAAESWSGTPSNLLLKINGNASLAEQR
                     YLPQSSKALSSHIERLTPMLRRHGVAVEKEKTRAGRLYRLWLLASDENFRPGGTVAI"
     gene            complement(28271..28513)
                     /locus_tag="PL162_01330"
     CDS             complement(28271..28513)
                     /locus_tag="PL162_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90702.1"
                     /translation="MKRITTVTTASLELRRRGMSVKDLAEVLERSANSLYPVVGRRRR
                     ATNPERVAIAAFLGAQVEALFDEDGFARVARVAANV"
     gene            28864..29193
                     /locus_tag="PL162_01335"
     CDS             28864..29193
                     /locus_tag="PL162_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90703.1"
                     /translation="MGLSLRRTQEIAQGAEIAHVSAFTNSYLTVTAKAWMDLNQLGRR
                     NLTKEKRDEMIRRLAARGVDKKEIAEKTDLSLRSVQAITQRKNCAEIHVNATTPAAEE
                     VTYYYGE"
     gene            complement(29829..30335)
                     /gene="gpt"
                     /locus_tag="PL162_01340"
     CDS             complement(29829..30335)
                     /gene="gpt"
                     /locus_tag="PL162_01340"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HQF90704.1"
                     /translation="MATEDRYRKTFPVTWDQLHRDCRALAWRLMGRRPWEKIVAVARG
                     GLVPAAIVARELDVRVIDTVCVSSYTLRNQGEIQLLQDTAHLRGCSEDWLIVDDLVDT
                     GNTARVVRRMFPEAHFATVYAKPDGRPEVDTYITEVSQDTWILFPWDAEIHVEFVSPI
                     AQQSKDPA"
     gene            complement(30465..31247)
                     /locus_tag="PL162_01345"
     CDS             complement(30465..31247)
                     /locus_tag="PL162_01345"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HQF90705.1"
                     /translation="MVILALLGALLGACLGSFLNVVASRSLAGEPWGGLSRSHCPHCG
                     RELPWSELVPLLSFALQRGRCRGCTRALSWRYPAVEMVGALLGGAVWGRWGLSPWALA
                     LGTVAAFSLFLNALTDLEEGVIFDLFALSPGALGLILRVGGGKDALIDGLLGAALGFG
                     IIAVVIVASRVLSGQEGMGWGDGSLMAGLGGILGWKMTGVAFYGGIMAGGVVIVPMMI
                     LGKVHRKDAIPLGPFLALGGVASLLGGPSLLAWMGWAPGWPW"
     gene            31519..33201
                     /locus_tag="PL162_01350"
     CDS             31519..33201
                     /locus_tag="PL162_01350"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HQF90706.1"
                     /translation="MKWKAMVAGALVTLGVAVGGARGEAREDWALTLLRGMSLEEKVG
                     QVMVGFFRGAELSGDLRDSLRRVRLGGVILYSSTGNIESPGQVARLVRDIQNFSARGG
                     LVPPWVAIDQEGGVVSRLVDGVTVFPGNMALGATGDGVLAEAQGRIEAQELRTLGITW
                     NFAPVVDVNNNPANPVIGIRSFGSSPEEVARLGAALGRPHYAAGVLWTAKHYPGHGDT
                     DTDSHLGLPRISGDLARLERVELAPFDALIREGVPAIMTAHVVVPALESGDSLPATMS
                     PAVLGRLRRQGFDGLIVSDSLGMGALDKTWGTPEAAVRALEAGCDVLVFGADKGHDPE
                     EQREIAQALIRAVRQGRIPEKRLDDAVLRVLRAKARLGIVKDPWPREGDLPLLAAPES
                     LAVADRIAQKSITLVRGGVLGPDERGEGSVPLVWPSDRRKAAEVLVEACPALAPIFMS
                     RDASPEERDEVIRRVGDAPRVVVGGYDLGRTPAWQALVQELGPVRVTLLALRSPYDLR
                     AVPAVGGYLCSYGDRPASLRALGGVLLGRVAPQGRLPVELPGLYPRGWGMGE"
     gene            complement(33290..33454)
                     /locus_tag="PL162_01355"
     CDS             complement(33290..33454)
                     /locus_tag="PL162_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression protein HypA/HybF"
                     /protein_id="HQF90707.1"
                     /translation="MRTFRCLECKHEFQEEDQKPQALKCPKCLSRFVQLVEGAPLKGK
                     QWGSKSYSVR"
     gene            33902..35626
                     /locus_tag="PL162_01360"
     CDS             33902..35626
                     /locus_tag="PL162_01360"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HQF90708.1"
                     /translation="MTRSNARARRRKASQAGSGKDTLRFFALGGLGEIGKNMYVLECG
                     DDIVVVDSGLMFPDEEMLGIDFVIPDISYLEENKHRIRGLVLTHGHEDHIGALPFVLP
                     KLDVPLYGTCLTLGMAQGKLAEVAPAYRAKLNEVKAGDVVELGAFSVRFIAVCHSIPD
                     GVALAIRTPLGTVVHTGDFKFDATPIDDRPTDYSAFAELGREGVLLLLSDSTNVERGG
                     FTPSERMVGRTLEQIFRLHRSKRIIMAAFASNLHRVQQMCDVSAMFNRKVVLMGRSMV
                     NNVELARRLGYIKADDDLFLPQHEADSFPANRTAVLTTGSQGEPFSGLVLMSKGEHRF
                     LNLGSKDLVVINATPIPGNEKLVSRTINRLFVCGCEVLYEQDRQLHVSGHASREELKM
                     MLSLVKPQYFVPVHGEYRMLVRHAQLAEEVGVNGKNVFVLQSGDVLEITRDKAQAKGT
                     VPAGGILVDGMALGEVEGSLLRERRELSEEGVVVVSLVVDRKFRLRGKPLVESRGAVH
                     VKESATLTKEMGDAVVQVVEGVSRRRDRSEKELAEAVGSRVRSVLRGYSRSSPTILPM
                     VTIVEEGA"
     gene            35632..36441
                     /locus_tag="PL162_01365"
     CDS             35632..36441
                     /locus_tag="PL162_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HQF90709.1"
                     /translation="MFGDSLVLGFVQGLTEFLPVSSSAHLVIMQRILGWREPALAFDL
                     MLHVATMMATLIFFRNDVGRLGMEWLAGFRGSEGRKPGWRYGWAVIAATMATALVGLP
                     LKPLVEKALSSTMVVGLGLILTAALLEGAMRASEGRRRLGLGHAFLVGLAQGVATFPG
                     ISRSGSTIAASVFLGIPREEAFRFSFLLSLPAILGATVLEGRDLGGWGAFGASLPSGW
                     WAGMAVALVTGLMALAVLRRLVIQGRWRGFAVYCALLGGSTVVFSLLFPGV"
     gene            36496..36738
                     /locus_tag="PL162_01370"
     CDS             36496..36738
                     /locus_tag="PL162_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90710.1"
                     /translation="MASRGIPWWLVILLVFFGTVVGVFLQRFDGLDTLFRDFARAGFD
                     LREVDLLFVRFGLSFFFRINLGTLLGGVVGLWLLLR"
     gene            36720..37343
                     /locus_tag="PL162_01375"
     CDS             36720..37343
                     /locus_tag="PL162_01375"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HQF90711.1"
                     /translation="MAFIEMSPLPPLVLASGSPRRRALLEELGWSFAVVPTEVDETPE
                     AGESPEAVARRLAEAKAAAGARAFPGALVLGADTLVALGDRFLGKPRHREEAEWMLSF
                     LQGREHRVVTGVALEWGPRKSSAVEVTRVRFRSLDPGAVRAYVATGEGDDKAGAYAIQ
                     GRGALLVEGIDGCYFNVVGLPLGRVSVLLEGWGVSLERQWTPGGEGA"
     gene            37340..39253
                     /locus_tag="PL162_01380"
     CDS             37340..39253
                     /locus_tag="PL162_01380"
                     /inference="COORDINATES: protein motif:HMM:NF039831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5693 family protein"
                     /protein_id="HQF90712.1"
                     /translation="MSRRILFFGGLGLALLSALWSVGHRWGAELERRQVALVVEYRDI
                     VAMAAEEHLPPEAVRERLQRAGLQGVVVGELTGEELSQGALPLWYGPLGSLPGEAGSK
                     SIDLPPNRGTLWLSGRSGALASAVEKYVALKFPDATERGGRSGKVWVLPQGVKELFEA
                     GVFPDVEGLSFAESQGLPTIYRPAPAPGVAPERVAALADAVLADHRHVVCLAPSGAVV
                     AGYPDLGALGKVLKSRGVDLAQVEFSAQIGGAALESGAFPRLLPLHSVTQDEVISKRL
                     WRPLLVDRMIRAAQERSVRLLLMRPYLLESGSRLTKLEQDLGSVAEALRRAGYALTWP
                     TPYSPGAQDFFGAWACALGALFGGIHFLWRFRGIQDRGIPGASLGVLLVGAALLAFMA
                     LRMPGVARWIGAAVAVITVTEASLEALEGWRTPLQALAKALLVLLAGGMAIAAFFGVP
                     EYMLRLKTFSGVKATLLLPPLLVLLHDFRRRIYPESLKSVLSRPPLWGELALLGGFFV
                     GLAILAVRSDNVSLAPQWEMALRSSLEQILVARPRNKEVFLGYPALVLWYAVRRADLS
                     PRYREVLRLCAVFAFASALNSFCHFHTPLYFTLWRVGNGFLGGLLVGGLLVAVGRWII
                     LPLWRRLAPAIMA"
     gene            39250..40287
                     /gene="csaB"
                     /locus_tag="PL162_01385"
     CDS             39250..40287
                     /gene="csaB"
                     /locus_tag="PL162_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR03609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046919 - pyruvyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase CsaB"
                     /protein_id="HQF90713.1"
                     /translation="MTSRSWAAALCGYYGFGNFGDELLAEALVRGMEECGVPRERLVI
                     LSAAPERSARDLGVASVDRWSFREVWGALKRSETLLLGGGGLFQDVTSLRSVLYYAGV
                     VHLARMAGCRPWAFGQSVGPLKSWIGRALARRSLGRCAFRGVRDAPSEALLRRWEMEC
                     RRSPDGAFLLADEGNPSPAETGKDLLVNLRPWGGSLSRDCAKIAAVEASRRRLSLVGV
                     ALAPEDEGVLEDLRRDGVFPCTRVVTLTDLVQARELFGGAAMAVGMRLHFLVISAVMR
                     RPAVAVPYDPKVESFARDWSFPVWYGEGPLPEPGGAEKDPGDARQEVKAALESAWLAS
                     SQEARRREVEA"
     gene            40284..41135
                     /locus_tag="PL162_01390"
     CDS             40284..41135
                     /locus_tag="PL162_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HQF90714.1"
                     /translation="MTSAPSAAETAREIRKDVVRMISLTGRGGLGISLALADLLTWLY
                     WSYLRVDPRNPRGEDRDRLVLGTGAGAPALYATLARRGYFSREELWNFSRLGAILQGV
                     PARLSIPGVDAPLAERGRALGVGCGMALGLRRKGSPRRVVCLLDGAAWQEGLFWEGVR
                     RCGAWGLGGVTAVVASSSEGISSAWSPSEAESALIQRGWRVLRTTGHCVDVLSREVED
                     MERKRTSEPLAVLAETTLGRGVRLEGERDERFHHEGRPWDRGVLGETLREIDGKKEGD
                     SGAPFVF"
     gene            41116..42063
                     /locus_tag="PL162_01395"
     CDS             41116..42063
                     /locus_tag="PL162_01395"
                     /inference="COORDINATES: protein motif:HMM:NF014799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HQF90715.1"
                     /translation="MLHSFSDASGAVERTFVEAFRRDERWMMVSSSSADALSRAIFRE
                     FPERFLAVDGEERSVVLTAAGLALEGFLPMVGPGSPRIVTRAYDEIRAAVALPSLPVL
                     LLGSGGGLFRGDLGAPDQVVEDLALMRTLPGMRVFFPSDASVLAEILAGPHDGPVYLR
                     WEPWGGDSSGSGGTSPREDLLAGGRIRRPGSAVTICTYGALVREALRAAEILSSQGID
                     AEVIECLSFKPLPENLILASGRRTGCGVVAEEHVTEGGLGAAVAELMGCYPIPVLSVS
                     LEGHFGQSGTEAELREYYALTRQHMVGAAVQAWSMRRGG"
     gene            42063..42758
                     /locus_tag="PL162_01400"
     CDS             42063..42758
                     /locus_tag="PL162_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQF90716.1"
                     /translation="MDIRLQGVSKIFPPDIRALEDVYLSIPQGEFVYLVGTTGSGKTT
                     LLRLISREAQITRGQIYAGSYNLKKIPTLSLAIYRRDIGVVFQDFKLLPDLTVMENVA
                     FVLEVIGVPPREISERVGEVLERVGMWRRRFLRPPQLSGGEQQRVAIARAVINSPAVL
                     LADEPTGNLDTRTAEDIMQLLLSINASGSTVIMATHNKYLVDAYRQRVVELRAGRIVR
                     DEKRGRYDVDGDF"
     gene            42745..43629
                     /locus_tag="PL162_01405"
     CDS             42745..43629
                     /locus_tag="PL162_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HQF90717.1"
                     /translation="MGTFRYALRDAFRLLGRHWGLALLTLVTATAVFFLVGGSALLVV
                     NTSRLVARVEGELVIHAFLKSDKNMEALREKVRQYPGVAEVREVTPAEAMDRLKKSIQ
                     NQAQALEILGENPLPPSLEIRVERAQDVPGVARDLLTLSSVDDVVYPGALASRLARVS
                     RVVVEFSLVILIIAVTASALVLFNTIRIAVYSKKEEISVMLLVGATPSFVAMPFVLQG
                     MILGVAGAAGALGLLGGAYRALVGGVETSVPFLEMVKDPDLLARLGLILVGVGMTVGW
                     VSSWLAVDRFIRRALRPI"
     gene            43693..44946
                     /locus_tag="PL162_01410"
     CDS             43693..44946
                     /locus_tag="PL162_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQF90718.1"
                     /translation="MICARVVVLGALFLGALPVGALRGQAWGAASADLKADDAASAED
                     LEKRLRTEEKRLADIQKRLKFHKGQVSVTQKKEKLTLSELSKLTDEIGEAQQQINVLE
                     LRTRQTKNKLAEVEAEIRATEDQIGAIRGTLAARLVAMYKYGDGAKIDMLLGAQDAQD
                     ALLTTFCLNRMTDQDRRLFASAVQAQQRLEEGRAKLLGHKTQLAIQQDRMRQERQRLG
                     QGIALRNAALEDLRSQKAKHATMARDLARLEEEIERIIRTLLQERQRMAEKSRKDRAD
                     QTGDKTLLLSAPSGPLMWPHRGKITSPFGSRIHPIFKTRTVHTGIDIDGNMGDPVKAA
                     ADGEVLYAGWLKGYGQIVILDHGGSLSTVYAHMSRINVEEGQRVRRGHVVGRVGSTGT
                     STGSHLHFEVRVDGNAKEPLRYLSR"
     gene            45046..46233
                     /locus_tag="PL162_01415"
     CDS             45046..46233
                     /locus_tag="PL162_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQF90719.1"
                     /translation="MGKRWRDVILGVFLGVALAGGVVAYAADVDLGQIVPFRAQSLWL
                     IKQARAILETYYVDAEEPVSEDVLVRGALRGMVSSLGDPYTRFTDPRQLHEEEIEMQG
                     EYGGLGIYIGQRDGATLVISPIDGTPADRAGLKPKDMIVKIGDTYILDWDQEKVVSHL
                     RGEPGTKVTIWVRREGEDELLRFDLVREIINIKAVRFEILSKDLGYIRLNHFNQKCVA
                     EMREALEKLKAQKARGLIFDLRNNPGGLLDAAVDISDMFLDKGIIVGMKGRVPKATDQ
                     IFATGGTLWDKPVVVLINEGSASASEIVSGALKDHRRAVVMGAKSFGKGSVQTLFPLQ
                     DGSGLYVTIAKYHTPSGFVIDHVGLSPDIVVEGTMDRELSKDQQLRAAEKELRRILTV
                     PRP"
     gene            46257..47267
                     /locus_tag="PL162_01420"
     CDS             46257..47267
                     /locus_tag="PL162_01420"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HQF90720.1"
                     /translation="MRGYLKKRKERLYAWAVVIFIFSLGGTLLGMAQWRRAHLDHYDD
                     WEKSIHEAVEMGEEETLITLIASDDLGDLLSSQRAGWVLEPSLLEEGNKHLSGEVPYR
                     YAREISCDLPRVAIVVDDVGYNAAMARKLAKIPIPLTWAIIPGLKGTRPCSKVADEAN
                     IPYMVHMPMQATGDKKEGRWYDLSWIVSGQSEAAVQKAVARALEELPDAVGMNNHRGS
                     LSTMDEALMVPFVKALGERRLGFIDSRTIRGSVAYKTARHHGVPAAYNSVFIDHYNDE
                     KFMKEQLDRLVMLAKRHGWAVGICHIRSGTLKMLAQLDVAQFGGVRFVTVPELFSCLG
                     RP"
     gene            47305..48591
                     /locus_tag="PL162_01425"
     CDS             47305..48591
                     /locus_tag="PL162_01425"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HQF90721.1"
                     /translation="MKGRVEVIVGSQWGDEGKGRVVDALADRVEVVARYQGGANAGHT
                     VIVGSEKFVFHLLPSGMLYTGKVCVIGGGVVVDPEQLLAELADLQAKGRDRARLVISG
                     TAHVVMPYHKLLDEAEEGQRSSGKKIGTTKRGIGPCYMDKISRVGIRMEDLLLPDVLR
                     DKLTFNLEGKNRLLTRMYDRRPLAFDEIYEKALGWGKCLAPYVGDASLVIAEALDEGK
                     GVLLEGAQGTLLDVDHGTYPFVTSSNPVASGGCTGTGIGPRKVDRVIGVTKAYCTRVG
                     EGPFPTEDLGPTGNYLREKGGEFGATTGRPRRCGWMDMVALRYAVQVNGMDAIALTKL
                     DVLTGMKEILVAESYEIDGVSTDRFSGAAHRLVQATPVYRALPGWQEDLSSCRRFDEL
                     PQAAQNYVTYLEEATGIPVVLIGVGPAREATILRGL"
     gene            48628..49107
                     /gene="rimI"
                     /locus_tag="PL162_01430"
     CDS             48628..49107
                     /gene="rimI"
                     /locus_tag="PL162_01430"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HQF90722.1"
                     /translation="MVLADIDFCALKDVDALEALEHLSFSHPWGRGIFAADLGRDRPG
                     ALYLGARLKGEFVGYGACSWTGDRLHILRLAVHPDVRRFGVGSQLLVALGEIGAAVPC
                     RRAFLELRRSNVAAATLYAHFGFVEVGFKKRYYSDNGEDALILEAPLPLREVPPKIP"
     gene            complement(49531..49605)
                     /locus_tag="PL162_01435"
     tRNA            complement(49531..49605)
                     /locus_tag="PL162_01435"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49571..49573),aa:Val,seq:cac)
     gene            50072..50542
                     /locus_tag="PL162_01440"
     CDS             50072..50542
                     /locus_tag="PL162_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90723.1"
                     /translation="MKISPWDDVPEYWAHWGFEPWSHGHLRGVRRKVIMRKGGLLGDV
                     ARYAVWDHILWGHRGRESAEEILREASPLPEVMTQRYVLVGDEEDPFRLRSFWWGFRG
                     FVEVLCYRPRGRGHRKFANLTGLVDALEGYVIRRKGEVTGTKGDGTESDGVGVS"
     gene            50574..52220
                     /locus_tag="PL162_01445"
     CDS             50574..52220
                     /locus_tag="PL162_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HQF90724.1"
                     /translation="MTMFIVTVVFFLAAYVLYGRFQVKLYGLDDSRKTPAEVYFDGID
                     YVPTHPAVLLGHHFSSIAGAGPIVGPITAASMFGWLPAYLWCLIGSAFLGGVHDMGSL
                     VASIRHKGLSVGEVVERWIGHRGKKLFLTFTWLALILVVAVFLELAAGTFAKDPAVAF
                     SGTLYMLLAIIFGVLIYRKGLSLLWCTLIMLPIVIGAVFYGSSSPWVAQLFSFGYDVA
                     TKEGQASLLDLWRIILIAYIFLASVLPVWLLLQPRDYLASYLLYFSVLIGAVGMIFGK
                     SFEPVLPAFMGFSNAKGEFIWPLLFVVIACGAISGFHALVGSGTTAKQLKKESDTLLI
                     GYGGMLIEGVVAVIALGTIIIAGLPADGSAVNPMAIYGAGIGKFASLLGIDLKVGASL
                     GLLALNSFILTSLDTATRLARYQFQEFFDMKVDRYTATAVGVAAALFLVFFRTGGKAA
                     WTLIWPVFGASNQLVAALTLLAVGVWVKHGLGKSATFLLAPMAFMFVTTVAALVLLIK
                     ANLGNPLLAGISAVLLVLAVLLVKEGWVALQRKTGEPLKG"
     gene            complement(52317..53489)
                     /locus_tag="PL162_01450"
     CDS             complement(52317..53489)
                     /locus_tag="PL162_01450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HQF90725.1"
                     /translation="MNPDFDLRHRREGTACAKWDGRREVFGTEDVLPLWVADMDFAAP
                     EAVMEALRSRVDHGIFGYPLRAQGAHNAFAAWMAQRHGWAVDPDWILEAPGVVPLLAL
                     AVQTFTTPGDGVVIQPPVYPPFRSVVERQGREVVENPLRWDGKRYVMDLEDLERRITP
                     ATRLLILSSPHNPVGRVWERQELESLGALCLRHGILVLSDEIHQDLLFSPHRHIPFAS
                     LGGDAARIALTFTSPGKTFNIPGLRSGMAVIQDETVRRRMKSTLEALDLEMWNVLGIA
                     ATEAAYTAGGPWLDALMTYLDGHRRLIQEELARTVPEVGYSLPEGTYLAWLDFRPLLL
                     CGEELRTFLVHRAHLGLNDGRSFGPGGDGFARLNFACPRTTLMEALRRLAEARSSL"
     gene            53733..54659
                     /gene="rbsK"
                     /locus_tag="PL162_01455"
     CDS             53733..54659
                     /gene="rbsK"
                     /locus_tag="PL162_01455"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019303 - D-ribose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HQF90726.1"
                     /translation="MAKVVVVGSLNMDLVMRTARLPQMGETWLGGAFSTAEGGKGANQ
                     AVACARMGASVALVGRVGRDDFGRRLVEALVVEGVDVGGVRFDDEHPTGVASIYVVEG
                     DNGIIVASGANAAVSARDVREARSAFEGAHCALFQLETPLEALGEGLRVAREAGAMTL
                     LNPAPWHALPGDFFPFVDVFIPNGIELGQFAGTADLEAGATKALEAGVGAVVVTVGSE
                     GAWLFRPGVKEHVAAPRVEAVDSTGAGDAFVGSLAVSLAEGLSLRESISFAVCAGALA
                     CTRLGAQPGLPRRKEVHALRRGWAGGEGGMRS"
     gene            54656..55051
                     /gene="rbsD"
                     /locus_tag="PL162_01460"
     CDS             54656..55051
                     /gene="rbsD"
                     /locus_tag="PL162_01460"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: protein motif:HMM:NF008761.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HQF90727.1"
                     /translation="MMRRGILHPELAALLAACGHLDEICVADAGLPIPPEMPRVDLAY
                     APGRAPFIDVVEVILGEMVFERALVAEEAATRSPEMLARLRGILGAVACESASHEEFK
                     NRVTRARLVVRTGEFTPYANVLLRCGVPF"
     gene            55064..56005
                     /locus_tag="PL162_01465"
     CDS             55064..56005
                     /locus_tag="PL162_01465"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQF90728.1"
                     /translation="MRRTPSSSRLPVLLAILVGIVALLGWHEPRFIEINNLINILRQS
                     SMVGLIALGMTLVILTGGIDLSVGSLVAVSALAGAGAARAGVTFPLCWGAALAAGALL
                     GMVNGLGVARLRLPPFIMTLGMMGFARGLAFMVTGGKPLTGFDASFRWLGIGGWMGLP
                     LPIWVFGGFFLVVWVLLELMPWGDQVRSVGSSPRAAWGSGVNVPGVTAQVYVLCGVAS
                     ALVGLLLAGRIDSAQPTAGVGYEFGAIAGVVFGGTSFSGGRGSLVGTVIGVLIMTVLE
                     SGMNMLNVNPFAEQVVKGVVIAAALVLYGRTGQALRG"
     gene            56119..56991
                     /locus_tag="PL162_01470"
     CDS             56119..56991
                     /locus_tag="PL162_01470"
                     /inference="COORDINATES: protein motif:HMM:NF007936.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose ABC transporter substrate-binding
                     protein"
                     /protein_id="HQF90729.1"
                     /translation="MMAAFAVLWLMAVPALADQPVLGLVVSTLNNPFFVSLKQGAEEA
                     AAKAQVSLVVLDAQDDSAKMTAAIEDLIQKKVAVILVNPTDSDAVTPSIMKANEAGIP
                     IFTIDRGASGGKVVCHIASDNVAGGKMAAEFLAEAIGHKGNVVELMGIPGTSAARDRG
                     KGFNDAIAAFADVKIVAAQAADFNRDKGLKVFENILQAQPEITGVFAHNDEMILGAVA
                     AAEAAGRTGIVFVGFDAVDDAVKAVQEGKLAATVAQQPALMGSTGVEKAVAFLGGASL
                     PEFIPVDLKLVVKE"
     gene            57079..58581
                     /locus_tag="PL162_01475"
     CDS             57079..58581
                     /locus_tag="PL162_01475"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HQF90730.1"
                     /translation="MSALLEMRNVHKRYPGVHALKGISFSLEEGEFHALVGENGAGKS
                     TLIKTLGGVVPPDEGGEILVRGERVAWSGPGDALRAGVGIIHQELSLAPHLSVAQNIF
                     LGRELRRGVFVDSRRMREMAKDLLAHLRCDIDPDRTAGELSTGEQQLVEIAKALSQKV
                     SILALDEPTSSLSARETENLFRVIDDLKSRGVGIIYISHRLEEIFAHADRVTVLRDGE
                     VTGRGAIGDFDPSSMVACMVGRRVEVLFPRSFRDPGEVLLEVRGISRRGVLRDVSFEL
                     REGEIVGVAGLVGSGRTETARCLVGLDPVDGGEVNLVGGALALRGPRETLDRGMVLVP
                     EDRKRQGLFLNRSIRDNATVMELLHGLRRGPFVDREKRDEAARRHVRNFSVKCRDIFD
                     PVFALSGGNQQKVAIAKGLAVAPRVLILDEPTRGVDVGARAEIHEIMNDLVHRGMGIL
                     MISSDLPEVLGMSDRVLVFRHGGIAASFDRGDATGEKVMLAAAGGGVHGG"
     gene            58571..59524
                     /locus_tag="PL162_01480"
     CDS             58571..59524
                     /locus_tag="PL162_01480"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQF90731.1"
                     /translation="MAGKWQRFGSLAVLIVVVVLLALVDRDVIDPKNVANVLAQSTVL
                     GVCAVGMTFVILTGGIDLAVGSVMALSGVVGALANTAMGLPWPLAWALGLGAGMMAGA
                     LSAALIAWGRMLPFIATLCVMAIARGITLLMTQGSPVSGLSPGFTHAGWASWGAVPIT
                     ALIFLLLATVAWVVLAKTPLGPHVRAQGDNPEAARLAGLSAPGLTFAVYMTSGFCAGL
                     AGLLMAGRLWSAQPAIGTGMELDAISAVVLGGTSLFGGVGSIGGTVTGTLIMGFLDNG
                     LRLQGVASYVQISIKGAVFVGAVILDLAMKGHYSRRRSRDA"
     gene            59648..60154
                     /locus_tag="PL162_01485"
     CDS             59648..60154
                     /locus_tag="PL162_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HQF90732.1"
                     /translation="MDESKTMKNLGEGFAGESMANRKYLAFAQQAEAEGFHRVAVLFR
                     ATAEAETIHALAHFKAMGKLKDTPANLQAAIDGETYEFTKMYPDFLVDAEAENNAAAI
                     RSFKLAKAAEEVHAELYEKALKNLEGEKAKAARFFLCPVCGHIAEDEAPEACPICGAK
                     KSAYKLYE"
     gene            complement(60220..61128)
                     /locus_tag="PL162_01490"
     CDS             complement(60220..61128)
                     /locus_tag="PL162_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQF90733.1"
                     /translation="MRENVRTLAADASLLFVAAVWGLGFAAMKGALESFPPFWLLTIR
                     FAAAALLIALIFPGRVRRITGKDLRGGVIIGTFLFLGFATQTVGLLYTTASKQAFLTA
                     VYVVLVPFFAWALKRVFPGIRPFGASALCLVGMAFLTLDLKGDFVINYGDMLTLACAV
                     FFAAHLLAIERFAPHLDPILLAFGQIATTAALSLVLALLWETFPTTISSTGFWSMAYC
                     VVFPTVMALLIQNTAQRYTPSTHVSILLSLESLFGALAGVFLLGEHLSEQMILGCAFI
                     FAAVLLTEVDPMPLVRRLLPKTDAAP"
     gene            61321..62175
                     /locus_tag="PL162_01495"
     CDS             61321..62175
                     /locus_tag="PL162_01495"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQF90734.1"
                     /translation="MRRSTLAALALGFSLLAGGAFANTSGDVSVGGTVQADPEKVLAV
                     VGKTEIKQQDVDGILAKLDPQRAAFFSTEDGRKKLLEELVNRELFLQWGVESGVDKQE
                     DVQKEIRSKTEDAIRNYALKGVLDAVTVTSEDIKKYYDEHQDEFKTPESVRARHILVD
                     SEDQAKQIVAELKGGLDFAEGAKKYSTCPSKEQGGDLGFFSAGQMVPEFEKAAFELAN
                     PGDMSEPVKTQFGWHIIKLEEKKPAGVAPLEEVREGLQQKVLMEAQKAAYDKKVEELR
                     QKFGVTMK"
     gene            complement(62362..63546)
                     /locus_tag="PL162_01500"
     CDS             complement(62362..63546)
                     /locus_tag="PL162_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HQF90735.1"
                     /translation="METNQQQYGFDLERYMPRERFERIKAFVADKDTPCLVIDLRRIA
                     AKYDELHQSLPYAKIYYAIKANPNDDVLKLLIDRGCCFDFASIYEMDQALRLGATPDR
                     LSYGNTIKKERDIAYAFSKGVRLFATDSDHDLQKLARQAPGSRVFFRILTDGSGADWP
                     LSRKFGAHPDAVYRLILQAADLGLQPYGISFHVGSQQRDIGQWDHAIAHCRYLFDAVR
                     DKGISLGMINLGGGFPAEYVAPTQSTATYAQEITRFLREDFGDNFPEILVEPGRFIAG
                     DAGVIFTEVVLISRKSGVSPNRWIYFDVGKFNGLIETMDESIRYPIYSEKEGPSEEAI
                     LAGPTCDSMDVLYENRKYALPLSLSEGDRVAILTTGAYTQSYCAVNFNGFPPLQMHVL
                     DD"
     gene            complement(63655..>64697)
                     /locus_tag="PL162_01505"
     CDS             complement(63655..>64697)
                     /locus_tag="PL162_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90736.1"
                     /translation="MIDTPWFRGLALGLFLLGGASAEGLALHPHNETLPLAPFRPDLS
                     SPWSGAARPGDQVLLPRDDLPESPGGLQVRLQGERHQRVITPEETPEGMCFAWPGAFT
                     SGEWVTVSLERSPSWEELYRQGRRWVQKHVSEGASFDEEGTPPPLPEAARMMALDEGD
                     PVIQRVTTDPGEKTLLLRGRNLRGSFTVHYPDGSSRPVSNDANANLRLPYGASATPGP
                     LSVTRDDGRPSNPVLVRFPRKVTGLLVLPEGASVDLGRVEIFPPSRLAQEKAVESPVL
                     RDDGAFVVGMAPGEELLGVFIRSPEGEGRVLVTSVAVPPEEDSLLVVLSPEDLARSLQ
                     GEKSPAENVEKP"
BASE COUNT        11608 a        19225 c        21619 g        12245 t
ORIGIN      
        1 ttttcgctgt cttcgtcgtt cagggcggcg gtgggctcgt cgaggatgag caggcgcacc
       61 tttttggaga gggccttggc gatctccacc agctgctgtt tgcccacccc cagatccttg
      121 gtgatggtgg cggggttttc ctgaaggcgc acccgtagga gcagctctcg ggtgcgggag
      181 aacgtctcct cccagtcgat gatgcctcgg cgggccctct cgttgcccag gaagacgttt
      241 tcggcgatgg agaggtaggg caccagggcg agctcctggt ggatgatgac gatgcccact
      301 tcttcgctat ccctgatgtg atggaaagca cacggcctgt cgtcgaagag gatttccccc
      361 tcgtaggacc cggcgggata gacccccgaa aggacgttca tcaacgtcga ctttcccgcg
      421 ccattttctc ccaccagggc gtggatctct ccctcccgga catccaggga aacgcccttg
      481 agcgcccgga cgccgggaaa ggttttggtg atctctttca tctgcaggag gattttcgcc
      541 atgttctcat ctcctgttcc gggggttagg cccccggaag ggggtccccc caaagggagc
      601 cccccttccg gggaggtgcc cggaaaaccc ctcaaaggac gaggggtttt ccgaaaagag
      661 ccggagggag aagacctact ggaggtcggc ttccttgtag tatccggagt cgaggagcaa
      721 cgtcttgtag ttgttgatgt cggcgaagac gggttccagc aggtaggagg ggaccacctt
      781 cacgccgttg tcgtaggtct tggtgtcgtt gacttcggga tctttcccct ggacgatgga
      841 gtccaccatc tcggcgacct tcgccgccag ggcccgggtg tccttgaaga ccgacatgga
      901 ctgtttcccc gcgatcatgt tctttacgtt ggcccggtcg cagtcctgtc ccgtgatgat
      961 gggccagtct ttgccggggg tgtagccgtt attggccagc gacgcggcga tgccgtaggc
     1021 cagagagtcg ttgggggaga gcaccacgtc gagcttcttg tccgtgtagg acttggaaat
     1081 gaggttgtcc atgcgggcct gggcgtcctc ggacttccat cccaggatgc cgatctgctc
     1141 catcttcgtc tgtccggact ggcacaccag ttggcccttg tcgaggtagg gctgaagaac
     1201 gctcatggcg ccgccgttga agaagaaggc gttgttgtcg tcgggggagc cgccgaagaa
     1261 ctcgatgtta aagggcccct tgccctccgt caggccgagc ttttcttcga taaacttgcc
     1321 ctgaatgacc cccaccttga agttgtcgaa ggtggcgtag tagctcacgt tgggggtctg
     1381 gcgaatcagc cggtcgtagg cgatgacggg gatcttggcg tccgccgcct tggccaggac
     1441 gtcggagagg gccgatccgt cgatggaggc gatgaccaac accttgcacc ccttggtgat
     1501 catgttctcc aattgggcga tctggttgct cacgtcatcc tcggcatact ggagatccac
     1561 gatgtaaccc ttctcttcca gggccttttt catgttggcc ccgtcctcgt tccagcgctg
     1621 gagggactgg gtgggcatgg ccacccccac cagcacgtcc gccgccaggg ccgccgtggc
     1681 ggaaagggcc agcaccaggg tcaacaggat aaagatcgct ttcttcataa ttaaactcac
     1741 gctccttttt ttgtgtgccg gggcgaaagg ggccccatct ctctttgaag ctcctggcgt
     1801 ccctcgtggg ggttcgccgc cgctcccccg ggtcggggga aagtctggcc gctttttcta
     1861 ggaagaggca tcaactcctt cttgaagggc tagagagccg gtttttcaag cgccaccgac
     1921 gggcgatggg tcccggatca gatcgggtag aggtgacggt tcaacaccga ttccagcatc
     1981 tcctggcgtc ccgaggcatt ttgtatgtgg tcgtgctcca gggcataggc ctccagggaa
     2041 gccaggtctt cttttccctg gcggatgcgg gcccccagcc cctcgtgcca tcccgcatag
     2101 cgccgggcca cgaagtcctc cagctctccc gaggctcgca gggaccacgc ggcgcggaac
     2161 ccccgggcga aggtgtccat ccccgcgatg tagccgtaaa agagatcccg gggctcgaag
     2221 gacccccgcc gcacccgggc gtcgaagttc aacccccccg gcgccagccc ccccgccgcc
     2281 agcacctgca tcatggccag cgtggcggcc cgcacgtccg tggggaattg gtccgtatcc
     2341 caccccagga gaagatctcc ctcgttggcg tcgatgctcc ccagcacccc gtgcacccgg
     2401 gccagggcta gctcgtgctc aaaggagtgc cccgccaggg tggcatggtt cgcttcgatg
     2461 ttgatcttga aataggggag caggtcgtat ttgcgcagga aggccagcac cgtggccacg
     2521 tcgaagtcgt actggtgctt cgtgggctcc ttgggcttcg gttcgatgag aaattgcccc
     2581 ttgaagccga tctgtcgggc gtagtctagg gccaggtgga aaaaccgggc catattgtcc
     2641 tgttccaggg ccagatcggt gttgagcagc gtctcgtacc cctcgcgccc cccccagaag
     2701 acgaagttct ccgcccccag ttcccgggcg atttccaaag attttttcgt cgccgccgcc
     2761 gcgaaggcga aaacctccgg gttacaggaa gtagcggcgc cgtggacata gcgcgggtgg
     2821 ctgaagaggt tggatgtgtt ccaaaggagc cggatccccg tggccttcat cagaccccgg
     2881 agcacctcca ccatggcgtc cagccgctga ttcgactccc ggagggaaga tccctcgggc
     2941 accagatccc ggtcgtggaa gcagaaaaag ttgatcccca gcttcgtcat catctcgaag
     3001 gccccttccg cccggcggcg gctgtggagc aggggatcgg cgatgccgtc ccaagggcgt
     3061 tgccaggtgc ctccgccgaa catgtccgcc ccttcggcgg tgacggtgtg ccaccaggag
     3121 agggcaaagc gcaggtgctc cttcatggtc ttgtccccga aggtctcctc ggcgtcgtag
     3181 aatcggaagg ccagggggtt tttcgatccg ggtccctcga aacgtacggg ccccacatcc
     3241 ggaaaaagat cttccatgat ccaatctcct ccttctcatg cggactttgt cgccaggggc
     3301 cggaaaggtt ccggggccct ttttaaccca ccgtcgtgcg ggcgtgacaa tccatggcca
     3361 ccatcgtggc tcccaagagg gctgaagcgt cgatgtccat ggaggcccga acgacgcgta
     3421 actttttcgc cgccagctcc aacacctgac gtcgcagtgt ttcttggatc tcttccagca
     3481 gggagtcggg ataatcggcg cccaccccgc cgagcatgac aaactccgga tttaaaatgt
     3541 tgcataggtt gcgcaggcct cgcaccaggt ggccgctcat ttggcacagc acctcccggg
     3601 ccacctcgtc gccctcttcc ctccggcgca gaatctcccg gtacttctgg gagttctccg
     3661 ggccccgcag gggtgtggag gcccgggcct catagtctcg gaccacggga tttatggaaa
     3721 cgaaggtctc gaagcatccc ctgtggccgc agtggcacag ggggcccgtg ggatccaccc
     3781 ccatgtgtcc cagttctccg gcgcagttgg acgccccccg gtgcaccgag ccccggagca
     3841 tgatcccccc tcccacgccg tgggcgaggt agacgtaaat tcccccctcc gccgccgcca
     3901 gctcgggatg gcggcgggcc tccgccgtga gcgatagttg ggcctcgttg gccaaaagga
     3961 gcagggcgcg gtgatcgggc aaacggcgaa ggatctcctg ggagaaatcg atatggtgcc
     4021 accccagatg ggggaaggat actcccatgc cggtttcggc gtccaccatg cccgggaggg
     4081 cgaaggagaa ggccaccagg cgctggtccg gcggcaggac gccttggatg catcggactt
     4141 cccgggcgaa ggtgtcaaag aactcctccg gatcggaagg ggtgggaaac tccgcccaaa
     4201 tctcatagct gttgtccagg tagccccggg ccagggcggt cctttccact cccaccttca
     4261 ccgccaccgc cactcctccg gcccggcgga gggccaggct caccgaccgt ctccccgctc
     4321 cgggggattt tttctccccc gtctcttcca ccagcccctg atccaaaaag agggatacgg
     4381 ccttggtcac cgtgctcgga tcgagccccg aggagagggc gagttcccgc cgactgatgc
     4441 cggggtggtg cctgatcatg ttgagcagcg tcagccgatt gttgtgcccc atgcgttcgg
     4501 cgttgaaggc agtcaacgga gctccccccc cttcgggttt gattgcttgt ttcaacaaat
     4561 atatcttggc gtttttgcgc cgcgttgtca agaggggtga agggatatgg gcgggctaat
     4621 cggcgggttc tggccgaggt tccgggccct ttggctcgga gtctcgacct ttggagggga
     4681 tctccccggc gtcccgaaag gggtctttgg gtcaggcggg gatggggagc tgtcccacca
     4741 gctcttcggc cacggccatg tgcagtacgt cgttgaggaa gaccaaaaaa cgctggtcgg
     4801 ggtccagatg gatggcggaa agggcgcagt tccccggcag gatgccgcga aacatctggg
     4861 tgggcatgga aaaaagggcc aggaggaggg cggagatcac gcctccatgg gccaccagga
     4921 ggatccgttc ctccggatct cctccggcga ggatggggtt gagggcctcc agggcccgtt
     4981 gctgggtaca ggcaatgggc tcccccccag ggggggtgaa cttcccgggg tctcgggccc
     5041 ataggttcag gctttctccc gcatcttgga cgagctgctc cacgggaagg ccctcccagg
     5101 cgccgaagct catctcccta aggccctcgg agacttcgca aggggggtgc cccccgcagg
     5161 cgcgtcgcac ggcctcggcg gtgtcccggg cccgaatcaa ggggctggtg acgatccggt
     5221 gaggtttcca aagggccacc cgagtcgcca ggcgggaggc ctgatgtctc ccctcgtccg
     5281 tcaagggaat atcgctacac ccttggaagc gccgctcttg gttccacgac gtcacgccgt
     5341 gccgagccaa aagaatcctt ttcatcaatc gtcgatcccc ctaaggggaa gccccctaaa
     5401 agggggactc cccatttttt tccggatggg cctactgtac cacgctttgg gccggaggaa
     5461 agaccttcgt ggttccttcc cttgacaggg gcgcggtaag ggggaaaata tccccggtcc
     5521 cttcgcgggg gccttctccg tccccccctg gccccggggc tacttcctcc ggaagagaaa
     5581 aatccccggg aggagtccca ggaggatgct ccatccgggg gccccgatgg cacaaccgcc
     5641 gccccctcct ccggaggttc ggggaggagt ggtgctggga cgaggtcctg tcgtgggcga
     5701 agggcttgcg gtgggtgaag gccccgccgt gggtgaagga cctgctgtgg gtgaaggacc
     5761 tgcggtcagg gcgttcacga tggtctgaca gagattgggg gccacccggc gggcaaaggc
     5821 ctcgttgggg tgggagtccg tgggggaggt ggcgtaggcg tccttgagat agagctcccc
     5881 gtcggtttcc agctggtaga agtcccagag gacgatgttg tccccgggtt cgtcccaggt
     5941 ggtgcggagc cactggacga aggcccgcat gcgcagggcc tgctcctccg ccaggttggc
     6001 ccgggtgtgc accgctccgg tccagaccac gaagagcgtc tggggatagg ccctcatggc
     6061 atcccgcagg gcaaggtact ggagcttgta gttttcctgg gtgcgggtgg aagaggtgac
     6121 gtcggggttc cccgtgtccg cttcgatctc gctcacgggg tagcagtgct tccagatcac
     6181 cacgtcgtac tgggcggtga gggtgagcag ggtggggctt ccccggaatt gggcctcggc
     6241 gccgtgttgc acccaaattt cccagtagtc gaagggagag ttatccccat cttcggggaa
     6301 atgctgttcg gagatgcgat gggaggtgcc atgggtccgg ttgtattcgt cgaaccactc
     6361 cggcacaccg ccgtaccaga tgacttcccc cgtgctgtgg tgtaaaagta ccatgcgggc
     6421 cgccgtggga gacgcgccct ggcccggccc ggccgccgtg aggatcgctc ccacggcata
     6481 aaagaccaac agaagaccct tgcgatccat aaccatcacc tccaaaaaga ttggcctgca
     6541 tcatggcgtt ggggcaaagt catcttagat ggtaccgccg acgtcgtcaa tagcggcgaa
     6601 tcgacccggg gcacggagac ggggggaaaa ttcatggcga ggggccccgg acccgctccc
     6661 tggccccggg cgacggagac ccctgggtgg agggagtgag ggaggcctct gaccgacgag
     6721 ggggatgaag ggtgtatcat gcggtgattc gggcggcgtc ttccgcccgt cgatggattt
     6781 tccccgacgt tccccagggg gggttgctcc atctttcgga taccccccgg gggacgttct
     6841 gtttcatcaa aaggttgatc ggccgcaccc ggcccgccgt gatcatccat accggagacc
     6901 tggtggacga catcaaactg gaattgcgcc cccgggagcg cgacgcctat gcccagcagt
     6961 tggctcgcct ccttcccata ctggaatctg caggggcctc ggtccatgtg atgctcggaa
     7021 accacgacga cgaggaagcg gtgcggaacc tcgggggggg cgtcgtcgtc catccccggg
     7081 aggtggcgct ctcctgggcg ggcaaggtcc tgcggggggc tcaccgggcc gaggacgccc
     7141 tcgagggtcc tgcgtcattt tatctcttcg ggcacgacct ttccgtgaaa agcagccctc
     7201 cggacgatcc ggtgcaacgg ctcaacggat tggaacacgt ccacctaatc tcccccgccg
     7261 gggaggtcct aaggcttcgg tggccctggg gcacggagga tgcccggctt ctgcggcatc
     7321 gctgcggcat ttgaaaggca aaaaaacggc ggggccctca aaagattggg ccccgccgtt
     7381 ccctttcggt cgtcttacgg tccttagtcc agcatgatgg aaagcatcat gcgttccact
     7441 accccctggg cgttcaggaa aaaggccagc tcggtgtacc cggcttcatc ctcgtagatc
     7501 atggctcgat cccgcatttc caagggctcg cccagctcac gcaccacatc ctggcgagag
     7561 ctccccaccc ggatgttttc gccgaaaata tccccctcct gttgggtgtc caccaggacg
     7621 aggaactctt tgtccggggc ccggaagagg gcgatgtggc gccgagggta ggtcaggtga
     7681 acccattcga aatgggccga ggggtcatgg gggctcacgt cggggctgat ttcttcgccc
     7741 gtgggggctc ccaggcggcg ggtgacctct tccacgctgt tgccgaaggc cacgatggtc
     7801 ttttccatca cggtgttgaa tcgctccagg gggtcttcac cccaggcttc gctcccgtag
     7861 agcgaaacga tcaaagccat cgccactact ccgcagctca ccttccgcaa tacgatcccc
     7921 tcctttgtcg tcgacgtccc ctagggacgg tggtaccgcg aatgggttaa agccctttcc
     7981 ccctcggggg tcacacgtag cgccgcagct cctccagggg tttgcggatc ttgggtcggg
     8041 gagggccgtc gtcggggtac cccagggtga ggagcagatc gacccgctcc aggggagaaa
     8101 gggtcaaaag gcgctttacc gccctttcgg caaaccaccc cagcatacaa gtgcctaagc
     8161 cctcttccgc cgcctgcagg gccagatgct cgcaggccat gcccagatcc aagagggcgt
     8221 agggaacccc tcggaagatc ccggccatcc gggcggccca tcgggacttt tcggtgaccg
     8281 ccaccaccag cacgggggcc gaggcggcga aggagttcat ggcgtagggc ccggagaggg
     8341 cttcccttac tatgctctgg cgcagctcgg ggctttccac cacgacgaag cgccagggct
     8401 gggagttgca ggccgaaggg gccaggcgcg ccgcctccag gcaacgctcc agggcctccc
     8461 gggggatggc ttcgggacgg tatcgccgga cgctacggcg aaggtgggcg agatcgagaa
     8521 aggccacggc cgttgccctg ctaggggcgg acccgcagag gttcttcctt gggcgccagg
     8581 gcaggggacg cgggagagcg tcccgctaat ctcgccagat cccctaagac gatgtgccgg
     8641 gtgtgttcgg tctttaccca ggttccctga tcgtaggcca ggaatagggc tttgaagagc
     8701 acggggagcc aggtgaggcc gaaccataga taggcggggg cgtagaagaa ataggacaga
     8761 tccaggcgtc gatgccgcaa ggagggcccc accactgcca cgaaaaatcc gtagacgaag
     8821 accatgccca gggagatggg cagggccccc cagccgaggc ccagatgggc gatgggctgc
     8881 acccaggggt gggcccaggt tgcttccggc ctaaaagtca ccaacagggc gcaccccacg
     8941 aggcacagga aggacagggt ggcctttgcc ggggcggcaa ggtagaggaa cagatccaga
     9001 tagcgcaggc gtcccgtgga gaaaaagcgc cgaagcaccg tcgggccgta gtgtccgaag
     9061 acccagaagt gcccttgcat ccaccgcacc cgttgggaga aagaaacccc caggtcggtg
     9121 ggtttttcat cgtagatcac ggcgtgctcg ttccagtgga cccttttgcc cgaaaggacc
     9181 accatggtgg ccatttccag gtcttccgtc agacttccca cgaaggccag catctggagg
     9241 aacaggggag tgcgcaccgc gagccccgta ccccccaggg tggcggaaag ggtcaggtta
     9301 ccccgggcgc gttgccagaa gcggttggta taccagtagg ccatggcata ggagcgggcg
     9361 agccagttgt cccgggggtt ttttacgtcc agcaatccct gcaccgcgtc ggcgtcgggg
     9421 tgggcctcga ggtaatcgtt catgcgggac aggaagtccc gctcggccag gttgtcggcg
     9481 tcaatcacca cgatggcgtc ggagtgctcc agggggacct gctccagggc ccatcggacg
     9541 ttttcggtct tgccgctccg gacttcggtg gtgcgcacca gcacccgaac gccgtagcgg
     9601 gacgccacct ccgctgtggc gtcttggcag ttgtcacagg agacgcatac ttcgaagcgc
     9661 tcccgggggt aatcctgatt ttgtaggctg tccagcaggg ggccgatgac ccgagcttcg
     9721 ttgtgcgcgg gcaccaaaac gaaaaaattc aagcgcccct gggcccgggg agagtcggta
     9781 aaacgccccc agccaaaaat gctcacggcg atctggtaga tcccgtcaag ggcgagaaaa
     9841 agcactgcca gggccaccat aaaggccgcc atgtcccaaa gagtaaacgc cgccgatcct
     9901 gtcacgcctt ccatgtgcct tccatgcctc cataggagtt cccgatctgt ggggcggggc
     9961 cccattcctc ccgggatggc ccgggaaaac ttttgtgcgc ccgatactcg gcctctggga
    10021 tgggcagtgc gccttgccct cccttccatg agtatagcac ctggaggggg gaaaccacca
    10081 ttctttcggg gatcttttgt gccgttgaaa ggcccgcgaa gatgcgctat tatgatacgg
    10141 aaaggcatcc cgggggattc gatgagatct gcagggaggc ggaggccatg acagacaaaa
    10201 acaaaagatc cgtttggcac ggcgaacgtc agggaaagcc ggtgctcgtt tttctcgcgg
    10261 ccgtggtggt ggccttgggc atcgcctacg cctccgtgtc gcttttgtcg gacacgggct
    10321 gggacaacct ggagatcgtg cgcatgttcc agaagcattt catggcgcga gagggggcag
    10381 gcaagtaggc cctttgcccc cggcccctta aaaccacaag gtacaggaca ataaaggctc
    10441 cgggcgaggg gattatcctc gcccggagcc tttacctggt gtcagcgatg gcgctaaatc
    10501 taaatccctt cgggcccttc gggcttactc ctccacgctc tgaacctcgg actggcgctg
    10561 ctcgatgacc ttcttggaga tgtctcccgg gacttcctcg tagtgagagt gttccatgga
    10621 gaaaaatccc cgtcctgagg tcatggaccg caggatgatg gcgtagcgga acatctctgc
    10681 caggggtacc tgggctcgga ccacctgcat gcgacccttg gcgtcgatgc ccatgatgcg
    10741 cccccggcgg ctgttgaagt ctcccatgac gtctcccagg tactcctcgg ggaccatcac
    10801 ctctacgttc atgatgggct ccagcaacac gggggaggcg ttcttcatgg cttctttgaa
    10861 cgacagggac gcggcgatct taaacgccat ttccgaggag tccacgtcgt gatacgaccc
    10921 gaagaccagg gcggcggaga tgtccaccat gggatacccc gcgaggtatc ccttgggaag
    10981 ggcctctcga agtccctttt ccacggcggg gatgaaggac ttggggacca cgccccccac
    11041 caccttgtcc aggaactcga agcccgcccc gcgctcccgg ggagagaact cgatgtgcac
    11101 gtcaccgtat tgcccccggc ctccggtttg cttcttatac ttcccctggg ccttggaggg
    11161 ctttttgatg gcctcgcggt agggcacccg gggagtggcc acttcgagct ccacgccgta
    11221 gcgctcccgg gtgcgggcga gcatgatgtc caggtgcacg tcccccatgc cggaaaggac
    11281 gttgtcgttg gtctcggggt ctttgcggaa agatagggaa ggatcttctt ccagcatctt
    11341 gtgcaccgcc gtggccagct tatcctcgtc cgcccggctc ttgggggtca cggccacgct
    11401 gtagacgggc tgggggaatt ccaggggagg aacgatgagc ttacaacctt tggttcccat
    11461 ggtgtctccc acagcggcgc tgtggagctt ggggatggcg acgatctctc ctacgacgat
    11521 ctcttttgcg tccttgccct cttttcccgt catgagcttg aaggcgctga tgcgctcgtc
    11581 ttcgcccttc gagacgttga aaatgttttg gtcgctggaa agggcccccg aaaggacctt
    11641 caggaaggag agcttgccca cgtagggatc caccatcacc ttgaagcaga aggccacgaa
    11701 ggggcccttg ggatccggga tcaagggagt ctcggtctcc ccttccaggg cgatccgggg
    11761 ggcgcgctcc atgggggcgg ggaagtactc cgcgaggagg tccatgagct gctccactcc
    11821 cacgtttcct ccgctctctc cggggatcac ggggaagatc aggcgatccc gcacggcctt
    11881 gcgcagggcg gggatcagct cgtccgtgcc gacctgttcg ccgtcgaggt agcgcatcat
    11941 gagctcgtcg tcggcctcca cgatgcgttc caccagggcg tcccgggcgg ccttcaccgc
    12001 ctccgtcatg tcgtcgggga cggggccctc tttgccgccc ttgctgccat cgcccttgta
    12061 taggtaggcc ttctcgtgga ggagatccac cacgcccttg aacgccgcct cggcgccgat
    12121 ggggaggtaa agaggggtcg ccttttcgct gagatatttt tgaagttccc ccacgatgcg
    12181 gaaaaagtcc gcgttttctc gccccatctt gctcaggtag aacgccacgg gaaggtggtg
    12241 ttccgccgcc gattcccagg cgttctcggt ctggacctcc acgccgtgta ccgcgctggc
    12301 caccagcacc actccgtcgg ccacgtacat gcaggaacgc atttcccccg tgaagtccgc
    12361 gtatcccggg gagtccagaa ggtagaaggt tttccccttg tgtggcacgg tggccagagc
    12421 ggcgttgatc gagatctggc gtttctgctc ttcgaggctg aagtcgaggg tggtgttccc
    12481 gtcttctatc ttccccaacc gggtggtcac ccccgcatcg aacagcactg cctctgcgag
    12541 tgtcgtcttt ccagcgccgc catggcctgc gatcacgacg gtccggatgt cctcaggttt
    12601 ctgggccata cccgtcctac ctcctcacgt cttttgccct tcgcgcgtct tttccggatc
    12661 ccggttcttt aaaaacgggt cccggctaca acccctccta tggtaccgga gggcgccagg
    12721 tacgtcaacg gaaaggggca aaaaaaatta gcgccacgct tccatctcgc ccctgctacg
    12781 gggataacgg ggataaaggg aaggatcttc tcgtcatggt gccggacagg ggtcctgtgc
    12841 ccatctttgc gcgggataaa agtcccgtaa ggcgatctta tttaccccct tgcccgccgt
    12901 ctccatcccc gtcttgtcat ccccgccttt tctcttaggg ggagtctagg ggggactcag
    12961 tgtagtcggc gagggcgtaa gtttacgaat cccctccgcg atggggccac acgccagatt
    13021 aatagagatc tcttcgagga actatggccc tttcggccct gcgcgccctt cccccaggcg
    13081 gtttatttta tttccggaga tacccggccc cctttcctca attcccgggg cgattttgtc
    13141 ggatttttct ctccataccc cttgcatcaa agggggaaat ggggtactct ctttcttaga
    13201 aaagacgggg tggtggatat tgacacttta ctcacatcgt cggtgtggtt tatttttatc
    13261 aaagcatatt taatcaagct tacctctcca aggatggcga tatgggaggg ggggagcttc
    13321 ccgctccggg tgcaagggat cttttttgag cttgatggtt tcttttggcg ggatttggga
    13381 aacagtggtc tggaggccgc gcaggggtgc ggcgatcatc atgggggagg tcgaacacgg
    13441 tgcgcaagga aaacgtgaag gtgatcattt ggggtctcgg cgccatgggc aagggcatgg
    13501 cgaagatgat gctcgaaaag aagggctttg agatcgtggg agtggtgggc cgaggagcca
    13561 agctcggcaa gagcatgtac gagttcctcg acatgccccg ggggagccgt cccgacgtgg
    13621 tcatcggcac cccggaagac gtcatcaagc ccggcgccgc cgacgtggtg ctcctttgca
    13681 ccgattcctt cgtgaagagc gccttcgata agattaagtt cgcggtggag cggaagatca
    13741 acgtgatctc cagcgccgaa gaaatggctt atcccaaggc ccaggagccc gagctggcca
    13801 aagagatcaa cgccctggcc aaggcccacg gcgtcaccgt cctgggcacg gggatcaacc
    13861 ccggcctcat gatggacctc ctcgtggtgg tctttaccgg cgcctgcgag accgtggagc
    13921 acatcacctc ccggcgggtc aacagcctgt cccccttcgg ccccgccgtc atggaggagc
    13981 agggcatcgg cctcaccgtg gaagagttca agaaaaagcg cgacgagggg catctggcgg
    14041 ggcacgtggg ctttgccgag tccatccaca tgatggccga cgccatcggg tggaaagtgg
    14101 acaaggtgac ccagagcatg gagcccatcg tcaccgaagt ggaccgcaag tccccctatg
    14161 gcttcgccaa ggcgggcgac gtggccgggt gcgccatgaa gggcttcggc tacgtgaatg
    14221 gcgagcttaa gatcgagatg gaccaccccc agcagatcga gccccatctc ggcggcaccg
    14281 acaccgggga ctacgtcatc atcaagggta cccccaacgt gaacatggcc aataagcccg
    14341 aaattcccgg cggcatcggc accatcgcca tgtgcgtcaa catgattccc cacgtcatca
    14401 acgcccgtcc cgggctgaag accatgttgg atctccccgt tccccgggcc atcatgggcg
    14461 acgtgcggga gatgatcgaa gaggagtagg cccggtagag gaccagaagc tctccccaga
    14521 ggcgttttgg gggttcgccg tgccccctgg aacgccccct tcgaagagag gaaaaaggca
    14581 taaaagggcg tgcggcgtgc ggtgccgtgc gcccttttcg tttggggcgg tttttttcgt
    14641 gaccccccca cgctatgggg tacataagga ggcgcgacgg aaatgcaggg cgcaaaaaaa
    14701 ggcgattggg ttcagatccg acaggtggtg cttccccccg aaggaagagc tccccaggtg
    14761 cccgaagaca cccggggcac ccccctcctc ctgtgggtca agggctttgc ccagagcgac
    14821 gccgccgtcg gggagacggt ggagatcgag accatgacgg ggcgtcgggt aaagggagaa
    14881 ctctgcgggg ccaaccctcg gtacatccac gactttggca acttcgtacc cgagtttctc
    14941 caggtggacc tccagctcaa ggccctaatg gaaggagaga tctcgcgatg accggtcgag
    15001 acatgtccta tgcggcggtg atgggccgca agaacgagat catgaagaag gccatcggca
    15061 tcgactacca gacctttgag tccggcggcc tggccttcga ctacgagcgc atgatgcgcg
    15121 aagccagcta ctccctggaa gaaattcaac agatccagta ctccacggga gtgggaaaca
    15181 cccccatcct ggagctcgcc aacctgacgg cgctggcccg caagttcgcc cctgcgggta
    15241 agggcgcccg gatcttcatc aaggacgaag cctgtaatcc ttcggggagc ttcaaggccc
    15301 gccgggccgc caacgcggtg taccacgcga aaaagcacgg ctacaagggc gtggtcaccg
    15361 ccacgagcgg aaactacggc gccgccgtgg cctcccagtg cgccatgcgg ggcctcaagt
    15421 gcatcgtggt gcaggagtgc tacgatagcc gcatgattgg tcagcccgaa atcctagaaa
    15481 agcagcgggc ctgcgaagcc tacggcgccg aagtggtcca gctcaccgtg ggccccgagc
    15541 tcttctacac cttcctgagc cttctggagg agacggggta cttcaacgcg tcgctttaca
    15601 cgcccttcgg catcgcgggg gtggagactt tgggatacga gctggtgacc cagttccggg
    15661 aaaagctggg ccgggccccg gagaaggtgg tcatcaccca cgcgggaggg ggcaacgtca
    15721 ccggcaccgc ccggggcctt ctcaaggcgg gggcgacggg caccgaagtg gtgggcgcca
    15781 gcgtggacct ttcggggctt cacatggcct ccgataagga ctttaaccgc aaatccttca
    15841 ccacggggca cacggggttt ggtattccct ttgccacttg gcccgatcgg gccgacgtgc
    15901 cccggaatgc ggcgcgcccc ctgcgctatc tggatcgcta cctcctggtg aagcagggcg
    15961 aggtgtttta catcaccgag gccctggcgg tgctggaagg tctggagcgg ggcccggcgg
    16021 gcaacacctc cctcaccgcc gcctttgccc tggcccagac catggatcga gatcatttca
    16081 tcgtggtgca ggagacggag tacaccggag cggggaaaca tccgttgccc cagatcagct
    16141 ttgccaagca aaacggcatc gagatcctct tcggggatcc cgataccgag gtgccgggga
    16201 aaaacatcgt cttccccgat agcccggagc gcatccgcct gcgggacttc gaccttgcca
    16261 aggcccgcac ttcctacatc cgaaacagcg tgacccaggc caagcgcgag agcatctcca
    16321 aagaggacct cgccttcctg gcggcggaga ccaaaagcga cgagaacttt gtcctgggag
    16381 tgctgaaaga actgaacgtt tcggtgaagt aggacatggc ggggggcccc cttaggggct
    16441 cccccgggac tctttttctg gagggatcgg catgaagaga gcggacgatt ttgcatctcg
    16501 acgccagcac ctggcgggac ttagcgacga agagctcaag aagcgcttct gggagctggc
    16561 agaaaagatc gtggagccca tgctggagct gggccgtacc cacaccactc cggcggtgga
    16621 gcgtagtgtg ttgctccgca tgggtttttc tagcctggaa gttaagccca tcgtggaggg
    16681 cgtcatcgcc aaggggctca tgggcaaggg cgcggggaac gtggtatggc gtctttccaa
    16741 aaagctcggg atttccgtcc gggaggcggg cgtggccctc tcccaggggc aacactggga
    16801 tgtcgtggac accctttttt aggataggga gaagatagcg atgaaactcc aacccaataa
    16861 gcgcctggac atcaaggaac tcctcaagga tctggagaac tatcgcccca agcggcgcgg
    16921 atggcactgg cgagagggcc ggaacgagcc ccgcatcgtg ggggacttcg agtaccacga
    16981 ggcctccgaa gggctcacca actacgtgcc cctgccggga agccggggct ttcactacat
    17041 cgacccccag cccgattgcg tcatcaccac ggagatcgcc tcggggcgtt tcgaggacga
    17101 cgtgcgtcgc atgcgcatgg ccgcctggca cggcgcggac cacatcatgg tgatccgcac
    17161 ggcggggcag tcccacatcg actccctgct ggaaggcacc acccagggca tcggcggcat
    17221 cgccgtcacc cgcaagcagg tgcgcgcctc ccgcaaggcc ctggatgcca tcgaagaaga
    17281 agtggggcgc cccatcaact tccactccta catctccggc gtggcggggc ccgatatcgc
    17341 cgtcatgttc gccgaagagg gcgtgaacgg cgcccaccag gacccccagt ataacgtgct
    17401 ctaccgtaac gtgaacatgg tgcggagctt cgtggacgcc tgcgaagcca agaagatcat
    17461 ggcctgggcc ggcattctcc agatcgacgg ggcccacaac gccaacgcca ccgccatgaa
    17521 ggcctggaaa gtgcgcccgg agctcatggt gcagcacgcc atcaacagca ttttctcccg
    17581 gaaggtgggt atcaagccgg agaacatcgc cctctccacc gtgcccccca ccgctccccc
    17641 cgccccctgc atgcgcctgg acctgcccta cgccgtggcg ctgcgggact tcttcagcga
    17701 atataagatg cgggcccagc agaacaccaa gtacatggaa tccgacatga gggaagccac
    17761 ggtgacccac accctcaacc tggtgatctc ccgcctcacc agcgcagacg tccagtccac
    17821 catcaccccc gacgaggggc ggaacgtgcc gtggcactac aacaacatcg ccgccatcag
    17881 caccgccaag caggccctga acggcctgga cggcctgagg gagatggtga agctcgaccg
    17941 ggagggcccc ctgggagtcg aagtccggga gctcaaggag cgggccgtac tcttcatgga
    18001 ggaaatgctt gaagtagggg gatacttcaa ggccgtggaa cagggctttt tcatcgacag
    18061 cggctactac cccgagcgca acggcgacgg cattccccgc caggccgacg gcggcatcgg
    18121 agtgggcatg ctcttcgagc gcgacaagga ttacttcgcc ccggtctccg tgcactacgg
    18181 ctacaacaac attcccgcag agttcggcgg caaggcttcc gccgccatcg gcggagacac
    18241 cttcgaagat ccctccaaga tcgtcttcat cgacgagctg gatgagaacg acaacgtgga
    18301 cgcccggctc cgggagcaga gtcgctacta cgaaacggag ttcatcaagc ccgaggtgga
    18361 gtggcgggcc gacggcgtgg tggccctgaa cctcttcctc cccgtggacg agcgcaccgc
    18421 cgagttcgcc gccattcgct gcggcgagaa gatgggcctc gaagatgtca ccgtggtcca
    18481 taagcaggcc atgcacccct ccgaagggac ctacgtggag ctcaaaggcc gggtgaactt
    18541 cgacatcaac gtgaacgacc tggtgattcc cgagaagatc cccgtccttt ccgaagagga
    18601 gatccgggaa gatatcgccg cgaaccccct tttcgtggtg gccgccacgg tgggtcagga
    18661 tgagcactcc gtggggctca aggagacgct ggacatcaag cacgggggag tggaaaagtt
    18721 cggcatcaag tacctctatc tgggaacctc ctgcccggtg gaaaagctgg tggatgccgc
    18781 catcgagacc aacgcgggag ccatcctggc ttccaccatc atcacccacg acgatgtcca
    18841 cgtgaggaac atgagaaaaa ttcacgagct ctgcgtggaa aagggcattc gtgacaaggt
    18901 gacgctcatc tgcggcggca cccaggtctc ccacgagatc gcccgagagg cggggatgga
    18961 cgcgggcttc ggccgtggca ccaagggcat cgacgtggcc agcttcatgg tgaaattccg
    19021 gaggggccaa gcgtaatatc atgaaaattg ccgtcctggt ggccgaaatc ggcagtacca
    19081 ccacggtggt gaacgccttc gacggcatcg gcggtccctg cccgaggttc ctcgggcagg
    19141 gacaggcttt tacgtcggtg ctcgggggag atgtcaccgt gggccttcga ggggccgtgg
    19201 aggatctgcg ccgtcatctg ggagtcagcg agatctcctg gggcgagatg tttgccacct
    19261 ctagtgccgc cggagggctt cgcatgagcg tccatggcct ggtgtacgac atgaccgtcc
    19321 gggccgccaa ggaagcggct ctgggggcgg ggggcatcct gaaacatgtc accgccggga
    19381 agatgcggcg gacggacctg aaaaaaattg tggaaatccg ccccaacatc gtcctggtgg
    19441 cgggaggcgt agactacggc gagcgagaaa cggccctgca caacttcgag ctcgtggccg
    19501 atttgggcct cgacatcccc gtgatctacg cggggaacgt ggaaaaccac gacgaagtcc
    19561 gcgagatcgc cgaagagaag aacctgcgcc tctacctggt ggagaacgtc tatccccgag
    19621 tggaccagct caacgtggag cccacccggg cggtgatcca gcaggtcttc gaagagcaca
    19681 tcatccacgc cccgggcatg ggcaccgtcc gggacatggt ggacggcccc atcctcccca
    19741 cgccgggggc ggtgatggag gccgccaaag tcctaaaaga ggccatcggc gacctggtgg
    19801 tcttcgacgt ggggggggcc accaccgacg ttcactccgt taccgagggg agcgaggaga
    19861 tcggtcgcct catggtgagt cccgagcccg tggccaagcg caccgtggaa ggggacctgg
    19921 gggtctacgt caacatgtat cacatcgtgg gcaaaatcgg ccgggaggag ttgctccggg
    19981 agtttcccga cctggactcc ctcctggcgg gggcccgggc catccctgac acggaccgat
    20041 ctaaggcctt cgtggagcgt ctcaccgaga cggccatgct caccgctctg gagcgtcacg
    20101 cagggaccct gcgccagctc tacatcgcca cgggcaaacg caccatcgcc gagggcaagg
    20161 atctcaccgc cgtgcgcttc atcgtgggca cggggggggc cctcacccgc ctcccccggc
    20221 atcgggagat tctggctcgg gcggcggggt acggcaaagg gatggagctt ttccccaagg
    20281 ccgaggctcg ggtgttggtg gataacgact atataatggc ttctctgggg gccctctctc
    20341 ggcgctatcc cgacgacgcc cggaagcttc tcctgaacag cctgcactgg gaggaagggg
    20401 cgtaggaccg tcgcgcgccc tacgactttg acggagcttt ttgcccttgg tccccgggcc
    20461 ggggaggtgc ggcggcccgg ggagccctgt tttttggcgg tggcttcgac cctcggcctc
    20521 gccggggccc gcgatgtttt cttcgttttt cggggcgtgg agggaatgat acggagtgtg
    20581 aggaggtttg tgatatgccc tatcccaagc ttgtgataga cacaaaaaaa ctggcccaca
    20641 acacccgggt tctggcggag cgctgtgccg cccggggcgt ggctcttgcg gcggtaacca
    20701 aagtctactg cgcctttccc gaactggccc gagtccaggc ggcttccggg gttgcctggc
    20761 tggccgacag tcgcctggag aatctggcca agatccagga tatccccgtg ccgaagatcc
    20821 tcttgcgcct tcccatgata tcccaggcgg aagaggtggt ggccctggcg gatctgagtt
    20881 tcaactccga gatcgccacc tgcaaggccc tcgccgccgc cgcccaaaaa cagggaaaaa
    20941 tccacgacgt gctcctcatg gtggatctgg gagacctccg ggaaggggtc tggcccgacc
    21001 gggccgtgga ggccgccgga gagatcctgg cccttgaggg gttgcgcctc cggggagtgg
    21061 gggtcaacct cacctgctac ggcggcgtcc tgcccagcgt ggagaacatg acccgcctgg
    21121 tggaggttgc cgaggccatc gaggctcggt acggcctttc cctcgacatc atttccgggg
    21181 ggaactcctc ctcgctcaag ttgctggctt cggggaaaat gccctcccgg atcaaccatc
    21241 tccggctggg ggagtcggtg gtcttgggct tcgagaccgc cgagggcacc cgcatccccg
    21301 gcaccgaggg ggacgccttc accctggccg ccgagatcgt ggagcttcag accaagccct
    21361 ccgtgcccgt gggggagatc ggccgggacg cctttggcca ggtgcccgtc tttgaagacc
    21421 ggggaatgcg ccgccgggcc atcgtggccg ccgggcgcca agaccttcgc atcgacgcca
    21481 tgagccctag ggacgataag atcatcatcc tgggggccag cagcgatcac atgatcctgg
    21541 acgtcaccga cagttcccgg gagtggcagg tgggggacgc cgtggagttc gatctggcct
    21601 acggcgccct tctggcggcc accaccagtc cctacgtgac caaggagttt atctaggccg
    21661 agccccgctc tcaggcagga agagcttgac agcccttcct gccctcgcta taatgctcct
    21721 cgttgcgatt gttccggggc tgtggcgcag agggagcgcg cttccttggc atggaagagg
    21781 tcgggggttc gattcccccc agctccacca gctagtaata gcaagggttt gaagcccctg
    21841 ctaccgagat aaaaggccga aaaaacgaca cgacataacc ggtcattcgc gcaatcagta
    21901 accggtcgtt taagagcgtg ttgaggtccg tccagcgtcc gcgtcctact aaaattttgt
    21961 aggaggtggg cgctgtgcgt tttgaaaatc ggaaaaggaa agcttcatct tctctcgggg
    22021 catctgtcga tcctcgaagc ctccaggatt acttggagta ccggaagggc tccggcctat
    22081 ccaaggccac gttgggcctg gatagggcta ttctggttcc ctttgcgcga gagttccccg
    22141 cgtggcacga agacatccgc gccgccatgg tgggatatct cgacatgtcc aggaaggcat
    22201 ggaccaggtc cacacgccta agggttttca agggcttcat aaagttcctg gttgaggagg
    22261 gggagctccc cgagaacgtc gatcccctcc ggggggtgac ggccccactt cctggcaaaa
    22321 ctgccaccgc cgccacgccg gagggcgtca gtgccttcct ggcggccctg ggggcgtcgt
    22381 gggagcacaa gcgccttgcc gtcctggttc agctccttgc agatacaggg ctccggaaga
    22441 atgaagcgtg ctcccttcgg tgggcagact atgactccgc ggcacggcgt gtcgtcgtca
    22501 gggcggagaa cgcaaaaacg cgcagggaga gaactgtgcc cgtgtcgggc gacatggtac
    22561 gagtgctccg gaagtaccgc gacaatctcc ccgcgcgcct ccaggagtcg gagtggattt
    22621 tccccctcag ccagaaagac catatccccg ccgccgctgt ggggcgccaa gtcaaccgga
    22681 tcagccacgc cacgggggtg ccgctccgcc tgcacagcat ccggcacatg gccgccacgg
    22741 cgatcatccg cgctactgga aacatcgccc tagccgccca actcttgggg cacagccaga
    22801 tttccaccac ggcaggcttc tacacgcact tagggctaga tgacgtccgc gcagcacagg
    22861 aacaggtcgg aaccctccgg agcctgaggg cggcccgcca tgtttagccc cgtgacgctg
    22921 ttctgccttc ccgccgaaga gcggtacttc gtgctccaaa ggagccttcc gcagtgcctg
    22981 gacattcacc gtgcggaggg cgtggtgtgg gggagcggag ttccggagct tggggagtcg
    23041 cgcacgcgtt tcctccgccg aactatgagc acggagcaac ggcaagcctt cgaggagctc
    23101 ttcccggagg gggtcgtggt gaatgtcgtg gttgtcgccg gagacgatcc ccgcaacgca
    23161 aggaagtttt ccaccgtgtc tctctccgga gggcaccggc cggacgctct tcccctcacg
    23221 ctggaagtcg agggggttcc ggagcccggc gaagggctga gcttcttcgt gcacaggtgc
    23281 atgacggcag agcaaagggc catgttggag gagtatttcc gtgatccctc gggggcgtcc
    23341 gtgtgcacgt gggaagtcgc cagcatttcc ctcgacgctg tgacggtgcg agtgtagcgt
    23401 aggggggggc aaagaccgat tgagggaaga actggcccgg gggcacgcgc tcccgggcct
    23461 ttgtgctctc ccttggcgca gtccagcgtg agaaggtagt ccttggtcgg acggccgccc
    23521 ttatcgatta aacctaggtt tttcccaatt tcgggaaaaa ctgtcacgta atcttccccg
    23581 ggcgttgtga ccaccgctcc ccgtttgatt cgccaaattt ggcggatcat cggtggcgga
    23641 gatggagtta acggcgggaa catgcgccgt tacggcgcct cactccgcct cctccacggg
    23701 gtctccgaaa atcacatgga tagtgggcac ctccgtggtg gacacttgca cgcgttccag
    23761 gaagtccccg caggagcggc ccaggagctc cgaagcccta agccggttct tcatgggttc
    23821 ctccgcgtcc cgcataacgg cggtccagaa ggcttgtctc tcctccctgg tggccaccgc
    23881 cgccgatgcc cgcgcctcct ccctggcctc gataagtccg actatttcgg ggttcttgag
    23941 catccggcac cctaccactt tggcggtttt ggggctgtac ccggcagcaa tcgccgccgc
    24001 ctgtgcatcg ccgcaatacg cttccacaaa agcccgttgc cgatagttca gcacgatttc
    24061 acctccaata accatagttt accaaccctt gcaaacactg gttccgcctc acacatgccc
    24121 ggaaaggcca tttttaggcc cctggagccc catgctttct cagaggctcc aggagttcac
    24181 ccgacacaag ttaggggcct tgtaggccat cctggagggt caccacgggg ccttgcggag
    24241 ctccccgccg tcgcgccgat gcgcatacgc cgccaaggcg tccagctccc gctgcctcaa
    24301 gtcgcggtac cctgcgagga tttttccggc cgtgtcgtgc cccaaaaccc gccggatgtc
    24361 cgaagcccct atttccaggt gctcgactgc ccaagagtaa cgtggttggt cgatgtcgtc
    24421 acctccttac gccgaagcac cgatttccgc ccgggtgggg gtaacctcca cagcacaggg
    24481 ggggggcgct tcaacgcttt aactcttggg tgtgacgcat gtgacggatt aacttttgaa
    24541 tccgtcacac gcggattgat gcagcgcaac actcgcgacg cttgtgacgg atgtgacgga
    24601 tgtttcaaga cttgagaaaa tttcttcgag tatcctcggg ggcgtgggag tgtccaaggt
    24661 ctatttttat ccgtcacatc cgtcacatcc gtcgcggtcc ttggggcgca acgttccgcg
    24721 cgtgacggat caagaaggct atccgtcaca tccgtcacac ggagctcctc cgccaccgcg
    24781 cataggtcct cactgtgtcc gcaaagcaca gccaaggggc gtcgatgccg tcagcctcca
    24841 agatgccgcg ccgctcttcg tacagctccc gctccacagg ggttagggtc ccctccagct
    24901 ccgcgaggag ttgcgcaggg tcctccaggg cttgccgcag ggcttgccgc tccgtgtccg
    24961 taagatgcac cgccgccttg gacaccacgc acaaggggag aacgcggagc tccccttcct
    25021 ggagaagggg cacaaggccc ctttccagga gttttcttgc cgttcgaagg gcggtcatat
    25081 tgccaccgtt cccccgggcc ggaagttttc gtcagacgcc agcaaccaaa gcctgtacaa
    25141 gcgtcccgcg cgggtttttt ccttctccac cgcgacaccg tgacggcgga gcatcggtgt
    25201 taagcgttca atgtggctcg acagtgcctt gctgctttga ggcaagtacc gctgttccgc
    25261 caaagacgcg ttgccgttga tcttcaagag caagtttgat ggcgttccgc tccacgactc
    25321 cgccgccaga tctcgcagcc gaacggctac gccgtcatct tccagggcat ccagctccgc
    25381 cacgcgtctt ccttgtgtca gtgcctgggt gtattccccg caccgccaag gcaaccgccc
    25441 gccaagctcc gccgcgagaa caaacttgtg agcgtctgcc atgcgttcca gggaatcact
    25501 gggggcgtcg tgggagtgcc gcagggcatc agatacggca tccagcaagg ccccctggat
    25561 tttaggcaac acggccctga agcgtgcttc aatctccgcc gcaggctttc ggtcctgctc
    25621 ctcgataggt tcgagctgca ccactatgga acggctcagc aagtcggggc ggtccaacca
    25681 tgagccccct atcccgttca agatcacggg gcgacacacg tcaagcacgg cttcctcccc
    25741 atctgtgtac agctttctcc gcaccagacc gccgcccgtg gccattctgc acaaggtgtc
    25801 tgacatgtcg tgcgataggg cggagacgtt gtcaaagact gacacatgct gattctgcgc
    25861 acttaccagc accgaagcct catcctgggg catcgcccgc agtgctggcg ttgtggggtc
    25921 gatgatcctc ttcaacatct cgctcaaggt gcttttagcg ctcccctgtt cgccgcgcaa
    25981 agccaaaacg gggtacgggc ctttgggcat ccagcaagag atgagccatc caactacccg
    26041 ccggaagccg ccatcatcag aattcacaaa ggggcgcaag agctccagtg atccgccacg
    26101 ctccggaacg gtcatctccc gctgtgttgt ggggcgcagg aagggaaggg catgatcttc
    26161 ttggagcgtc cagcctgccg ccgaaacctc gatagagttc catccttgcg ttcccatgtc
    26221 caggaaaacc cggtcatcag tccagcctat gcgcacgaag ctttcccggg ggggggccgc
    26281 gtctgtcttg gcccccagga gcagctcgag ctcatccaat gcacccgcgg atggagccgc
    26341 ctcctcttgt tcaaagtaca ctgccgtgat ccagcgccgg aaagcatcgc tccccacggg
    26401 aaccagttcc cagtgtccac ctcgagggaa tcgcaccaac acacggccca gggtgtcacg
    26461 gaaggcggtc accccgaagt cctcacacat ttggagcaac gccgtggaaa gtttttcctt
    26521 ccgctccttc cgctccgaag gggcaacagc aaaggtttcc caactcatcg ggaacaccgc
    26581 accgcttcaa gagtgcgccg ctgatagtcg ggccgttgcc atttctcccg ctgtgccagt
    26641 gcggagcgtc caaagagctc cattgcccgg gtttcatcgc cgcgcgtcca gtgcaggagt
    26701 cgggccaaca accagcaatc cgcttcactc tgagaagggt agtacgcttc ccatgctcca
    26761 gagaaaaggt ctgcgagttt cttcccgtga gcactcgccc gggccttgag gaggagggcg
    26821 tcattatcca actccacagc tccacaccgt gcccgctgaa gggctttcgg ctgcggcctt
    26881 tggggcgcca ctaaccgggc tacgtggtta agggcttttt ggttcgtttt gattcgctcg
    26941 aaacccggca gaacggcgcc ggtccaacac aggtaccgct tggagccgta aacctccagc
    27001 tcctccttgc gctttccggg ccaaggcagc gtgccacgta ccagcacgtg gagccccgtc
    27061 cccgacacgc tccgctccgt gtaggaactc aagcgctgca caatctccat agctacaggg
    27121 tggatattgc cgccttgcat cacgtcatca aggtcaattc cacacaagcc gtctccggtc
    27181 agtaccagcc ccacgccgtt gtatccgccc gcgccgtacg ccgctcgcac gtcctcgaaa
    27241 cgcgcccagg ttctggggtc cgtggcactg gcacggcatc ccgtcactgc gtagggcacc
    27301 ttggcctttt tcggtctccc cacgtcctca gccttccaga gtacccaagg gcgggtcttc
    27361 agggcttcgg ggcagctctc aaagcatggg gcaagatacc cgtcaggccc cgccgccgga
    27421 gggcgctccg ctgtgtccgc cagaaagtat ctcgaggcaa tgccgccgga agctgtctca
    27481 tagggccggt cctcaatggg ccaaccttgg cgccggagct ccagcacacg cggggccaag
    27541 cgtccaatgc cgtatttctc cgccgccgct gcttgagtaa gcccctcgcc cgtctggaga
    27601 tggcgtagta ctgtctcacg ttgtgtccgt gcctgttctc cgcttgtcat ctcgccacct
    27661 ccagaagggc ataggccaac agtgcgcgca acccggaggg aaggcgccat gggctcgatt
    27721 gaaaggaaag gtctaccgtt gtggaatcat gcgaagggtg gccccatgtt ggggccgggg
    27781 gtaagtcgga tgagccggag ccgtcccctc cagggggcgc ttccacccgg caagcccgcc
    27841 gggtacgccg tgtggcccgg gggcgggggg cccgggtatg atgggcccgg gggcggggag
    27901 cgctgatcag gtggaaccgg acgcggcact ctacgaatag agcaaatatg tctctctgtg
    27961 tggcatgcgc agccccaagc cgccgcccga agtcatcgag tgcccgttgt tcccgtgccc
    28021 ttcgcgcaag gtgccgccgg taggccgtga cctttttcgt tgctaggctc ctccgccaga
    28081 gaacgcggaa gggcaccttg acgccgcagg ggctttctgg tattctggtt ccaatttggg
    28141 tcatatttct cctttctccc ccggcggccg gccgggggtt ttcttttgtc atcgggccac
    28201 cagggcgacg acagcaaatc cgcccagtat tgccacgcca acgcaccatc cgaacaggag
    28261 cgccgtcgcc tcagacattc gccgctaccc gcgctacccg cgcaaagccg tcctcgtcaa
    28321 agagcgcttc cacttgagcc cccaaaaacg ccgcgattgc tacccgctcc gggtttgtgg
    28381 cccttctccg gcgtcccacc accggataga ggctattcgc gctccgctcc aacacttccg
    28441 ccagatcctt cacgctcatt cctcgccgcc gaagctccag agacgccgtc gtaactgtgg
    28501 tgatcctctt catgatgaaa ccctcctatt tctgagatgc ccccattata gcctacttcg
    28561 cttgaaacgg actcttcagt ggagctcttt ctgcaagatg aggcttaggc ctcttgttct
    28621 gacggatgaa cttgcgtatc acgcaattct gcggataatt tgccacgggg acgccgcccg
    28681 cggcgggagt gcggcagaag gacattgcgg agaaggtcgg actgtcaaaa caagctgtat
    28741 cgaaaattgt ttcgcctgag tccactaagt ggactgaaaa acagattaac gccgtttctc
    28801 cctccgccga aattgaggcg ctgcaacgca agctcgaaga ggcagaagga cattgcggag
    28861 aaggtggggc tgtcgctccg gcgtacacaa gagattgccc aaggcgcgga aatcgcgcat
    28921 gtttccgcct tcacaaactc ctatcttacc gtcaccgcca aagcgtggat ggacttgaac
    28981 cagctcgggc gaaggaacct gaccaaagaa aaacgggacg agatgatacg gagactggcg
    29041 gcaaggggag tagacaaaaa agagattgcc gaaaaaacgg atctgtcgtt gcgcagtgta
    29101 caggcaatca cacagcgcaa gaactgcgct gaaattcatg ttaacgccac cactcccgcc
    29161 gccgaagagg tgacatacta ctatggcgaa taacgcacac attggggaaa ggggggcatg
    29221 actatccctg accaaggggg gaggaggggt tgacggtgac cggttatgtc gagtaaaatc
    29281 gggccaagta ggcgcttcga gggaacagga ctttgggcct tttttaacgg tagcgatttc
    29341 cgtaaacgct tgcagcgcaa gggttcgcgg atttttgggg cgtccgcttc cttggcatgg
    29401 aagaggtcgg gggttcgatt ccccccagct ccaccagtaa tagcaagggt tcgcggggtt
    29461 tcctcgtgag ccctttttct tttttcccta ctttcgcatt gccccggtca tttagattaa
    29521 agatcggcgt ataccagaga cacacgacca gaatccacag gggctcacgg cggtgaaccc
    29581 ccttcttctg ctgggggcgc gcatctttca gcgggatccc tttggtcttt ttaaccacac
    29641 tccatcctcc actcttttgc cccttcgggt ggtctgacgg agcggagatc atggagggag
    29701 cgggaacatc caagggccca cgatcctcag gttgattctc ccgccccacc actccggaaa
    29761 ttcccgtccc tcggtcgatg acttgtcccc gggggcttcg atgccttctc gccaaggctg
    29821 gggaacggtc aggcggggtc tttactctgc tgggcgatgg gggagacgaa ttccacgtgg
    29881 atctccgcgt cccaggggaa gaggatccag gtgtcttggc tcacctcggt gatgtaggtg
    29941 tccacctcgg gacggccgtc gggcttcgcg tagaccgtgg cgaagtgggc ctcggggaac
    30001 atgcgccgca ccacccgggc ggtattgccc gtgtccacca ggtcgtccac gatgagccaa
    30061 tcttctgaac accctcgaag atgggccgtg tcctggagaa gttgaatctc tccttggttt
    30121 ctcaaggtat agctggagac gcacaccgtg tcaataactc ggacgtccag ctctcgggcc
    30181 acgatggcgg cgggcaccag ccctccccgg gccacggcga cgatcttctc ccagggacgg
    30241 cgccccatga ggcgccatgc cagggctcgg caatcccggt gaagctgatc ccaggtcacc
    30301 ggaaaggtct tacggtaacg atcttccgtt gccatgctgt tggactcctc ccactaatat
    30361 cgaattttta caaagtctta tgcggccacc gaaagatctc cgggggctgc cccccgtctc
    30421 ccgaaaggtc ctcgccattt cccaatcctg ccggatggcg ccccctacca gggccacccc
    30481 ggagcccacc ccatccaggc caggagcgag ggccccccta ggaggctcgc cacccccccc
    30541 agggcgagaa agggtcccaa gggaatggcg tccttgcggt gcaccttgcc caggatcatc
    30601 atggggacga tcaccactcc ccccgccatg atgccgccgt agaaggccac cccggtcatc
    30661 ttccacccca ggattcctcc caggcccgcc atgagggacc catctcccca tcccatgccc
    30721 tcctggccgc tgaggacgcg gctagccacg atgacgacgg cgatgatgcc aaagcccagg
    30781 gcggccccga ggaggccgtc gatcaaggcg tctttccctc ctccgacccg gaggatcagg
    30841 cctaaggcgc cgggtgatag ggcaaagagg tcgaagatga ccccctcttc caggtccgtg
    30901 agggcgttga ggaacagcga gaacgcggcc accgtcccga gggccagggc ccagggagag
    30961 agcccccagc gcccccacac cgcccctccc aggagggccc cgaccatctc caccgccggg
    31021 tagcgccagg agagggcccg ggtgcaaccc cgacaccggc ccctctggag ggcaaaggaa
    31081 agcaagggga cgagctcgga ccaggggagt tccctgccgc agtgcggaca atgggaccga
    31141 gagagccctc cccagggctc ccccgcgagg gatcgggatg ccacgacgtt gagaaaagat
    31201 cccaggcagg cccctagcag ggccccaaga agagctaaaa tgaccatccg ttttttcctc
    31261 ctcgtcgtgc ggcggacgtc ggggggcgct ggggccccat cctccaccct cgaattggac
    31321 ggccgaaggg gagtccttcc gtgccaccta catcacttat accatggctt ctcctttgcc
    31381 tcaattgacc tccggaccgc ccctcgggaa aaaggtcagg agggtaaggg gggagatggg
    31441 cccccggagc ccgagggagt gtgctacact tttcccccgg aaaatggcgt cgacaaaaga
    31501 cgatgggggc gatgatcggt gaagtggaag gcgatggtgg cgggggcctt ggtgacactg
    31561 ggggtcgcgg tgggaggggc ccggggggag gcccgggagg attgggcgct gacattgctc
    31621 cgaggcatgt ctctggaaga aaaggtgggg caggtgatgg tggggttttt tcgaggggcg
    31681 gagctctcgg gagacctccg ggatagcctt cgccgtgttc gcctgggggg ggtgatcctc
    31741 tactcctcca cgggaaacat cgaaagcccc ggccaggtgg cgagattggt gcgagatatc
    31801 cagaattttt ccgcccgagg aggcctagtg cctccctggg tggccatcga ccaagagggg
    31861 ggggtggtgt cccgcctggt cgatggagtg acggttttcc cgggcaacat ggccctgggg
    31921 gcgacgggag atggggtcct ggcggaggcc caggggcgca tcgaggccca ggagcttcgc
    31981 accctgggga tcacatggaa cttcgccccg gtggtggacg tgaacaataa ccccgccaac
    32041 ccggtcatcg gcattcgatc ctttggctct tccccggagg aggtggcccg gctgggggcg
    32101 gccctggggc gtccccacta cgccgcagga gttctttgga ccgccaagca ctatccggga
    32161 catggggaca cggacacgga ttcccacctg gggcttccca ggatcagcgg agacctggcg
    32221 cgcctggagc gcgtggagct ggcccccttc gatgccttga tccgggaagg agtccccgcc
    32281 atcatgacgg ctcacgtggt ggtgcccgcc ttggaaagcg gagattccct gcctgccacc
    32341 atgtccccgg cggtgctggg acgcctgcgt cgccagggct tcgacgggct catcgtgagc
    32401 gattccctgg gcatgggggc tctggacaag acgtggggca ccccggaggc ggcggtgcgc
    32461 gccctggagg cggggtgtga cgtgctcgtc ttcggcgcgg ataaggggca cgatccggaa
    32521 gaacagcggg agatcgccca ggcgttgatc cgggcggtgc gccaggggag gattccggaa
    32581 aagcgcctcg acgacgcagt gctccgcgtc cttcgggcaa aggctcgcct ggggatcgtg
    32641 aaagacccct ggccccggga gggagatctc cctctcttgg cggccccgga aagtctggcg
    32701 gtggcggatc gcatcgccca aaaaagcatt acgctggtgc ggggaggggt gttgggcccc
    32761 gatgaaaggg gcgaggggag cgtccccttg gtttggccct ccgatcggcg gaaagcggcg
    32821 gaggtcttgg tggaggcctg tccggccctc gcgccgattt tcatgtctcg ggatgcctct
    32881 ccggaggaga gggacgaggt gatccggcgg gtgggagatg ccccccgggt ggtggtgggg
    32941 ggatatgacc tgggaaggac ccccgcctgg caggccctgg tgcaagagtt aggtcccgtc
    33001 cgggtgaccc ttttggccct gcgctccccc tacgacctac gggccgtccc cgctgtgggg
    33061 ggatatctgt gcagctacgg agatcgcccc gcttcgttgc gagccctggg gggggtcctc
    33121 ctgggccggg tggcgcccca ggggcgactg ccggtagagc tcccggggtt gtacccccgg
    33181 ggatggggga tgggagagta ggccttcttt cgttcggctc ctcggcgcga aagggagggc
    33241 gggaaaaaaa ccgggagggg ctttaccccc tcccgtgctc tttcttcgtc taacggacgc
    33301 tgtaactctt gcttccccac tgctttccct tcaatggggc accctccacg agctggacga
    33361 atcgactcag gcacttgggg catttgagcg cctggggctt ttggtcctcc tcctggaatt
    33421 cgtgcttgca ttcaagacac cggaacgttc gcatgccatc tcacctcctt cgggaatggg
    33481 gccttactcc ccgcattata cggttttttc cctccgaacg ccatccccgt cggagagcgc
    33541 ttgaccggaa gtgcggggat gggtagtatg gcccagagag gaatgtgggt gacctgggtc
    33601 accgctgttg tggtgtggcc ttcgaaaaaa ctaagtcatc ggggaggcaa aagggctcgg
    33661 gcccctgttc gaggcttctc ccgtctccgg gggggagaag cggggatttg ataactcccc
    33721 caggcgtagg caaagagagc aaagggggcg cccggggtcg ccggagcttt ttgaggcgcg
    33781 gcggggacgg aagaccccgg gccttggggg tcgcaaaaaa taacggataa aaccgggagt
    33841 tctgccccgg agaaaacggc ggaacgggat aaaattccct aatggtttca gaggaggatg
    33901 catgactcga tcgaatgcgc gggcgagaag aaggaaggct tctcaggcgg gtagcggcaa
    33961 ggataccttg cgctttttcg ccctgggagg tctgggggaa attgggaaga acatgtatgt
    34021 tttggagtgc ggcgatgaca tcgtagtggt ggattcgggg ctcatgttcc ccgacgagga
    34081 gatgttgggc atcgacttcg tcatccctga catctcctac ttggaggaaa acaagcaccg
    34141 tatccgggga ctggtactca cccacggcca cgaggaccac attggggccc ttccttttgt
    34201 cctgcccaag ttggatgtgc ccctttacgg cacctgtctg acgctgggca tggcgcaggg
    34261 gaagctcgcc gaagtggcgc cggcgtatcg agcgaagctg aacgaggtga aagcgggcga
    34321 cgtggtggag ctgggggcct tttcggtgcg attcatcgcc gtgtgtcatt ccatccccga
    34381 cggcgtggcg ctggccatcc gcaccccctt ggggacggtg gtgcatacgg gggacttcaa
    34441 gttcgatgcc acccccatcg atgaccgtcc gacggactat agcgcctttg cggagctggg
    34501 gcgggaggga gtgcttctgc tcctttccga ttccaccaac gtggagcgag ggggtttcac
    34561 gccgtcggag cgcatggtgg ggcgcaccct ggagcagatc ttccgattgc accgatccaa
    34621 gcgcatcatc atggcggcct ttgccagcaa tcttcaccgg gtgcagcaga tgtgcgacgt
    34681 gtctgccatg ttcaaccgta aggtggtgct catggggcgg agcatggtga ataacgtaga
    34741 gctcgcccgg cgtctggggt acatcaaggc cgacgacgac ctcttcctgc cccagcacga
    34801 ggcggattct tttccggcga atcggacggc ggtgctcacc acggggagcc agggagagcc
    34861 cttctcgggg ctggtgctca tgagcaaggg agagcatcgc ttcctgaacc tgggatccaa
    34921 ggacctggtg gtcatcaacg ccacgcccat cccgggcaac gagaagctcg tgagccgtac
    34981 tataaaccgg cttttcgtct gcggatgcga ggtcctttac gagcaggacc gccagttgca
    35041 cgtctcgggg cacgcctccc gagaagagct gaagatgatg ctgagcctgg tcaagcccca
    35101 gtacttcgtg cccgtccacg gggagtaccg catgttggtg cgacacgccc agctcgccga
    35161 agaggtgggt gtgaacggca aaaacgtctt tgtgctccaa agcggcgacg tgctggagat
    35221 cacccgggat aaggcccagg ccaaggggac cgttcccgcc ggaggcatct tggtggacgg
    35281 catggccttg ggagaggtgg aaggaagcct tctcagggag aggcgagagc tctccgaaga
    35341 aggagtggtg gtggtgtctc tggtggtgga ccggaaattt cgcctccgag ggaagccctt
    35401 ggtggaaagc cggggggcgg tgcacgtgaa agaatcggcc accctgacca aggaaatggg
    35461 agacgccgtg gtccaggtgg tggagggggt ctcccgccgt cgagaccggt cggaaaagga
    35521 gctggcggag gcggtgggtt cccgggtgcg ttcggtgctt cggggatact cccggagctc
    35581 tcccaccatc cttccgatgg taaccatagt ggaggaaggg gcgtaggcgg cgtgttcggt
    35641 gatagcctcg tgttgggttt tgtccagggg ctcacggagt tcctcccggt gagcagctcc
    35701 gctcacctgg tgatcatgca gaggatcctg gggtggcgtg agcccgccct ggccttcgac
    35761 ctgatgctcc acgtggccac catgatggcc acgctgatct ttttccgaaa cgacgtgggg
    35821 cgcctgggga tggagtggct agcgggcttt cgggggagcg aggggcgaaa gcccgggtgg
    35881 cgctacggat gggcggtcat cgccgccacc atggccaccg ccctggtggg attgccccta
    35941 aaaccgctgg tggagaaggc gttaagttcc acgatggtcg tgggcctggg gttgatcctc
    36001 acggcggccc tcctggaggg cgccatgagg gcttccgagg ggcgccgtcg tctggggctg
    36061 ggccatgcct tcctggtggg attggcccag ggagtggcga cctttccggg aatttcccgg
    36121 tcggggagca ccatcgcggc gagcgtgttt ttggggattc cccgggaaga ggcctttagg
    36181 ttttcctttc tcctttctct cccggccatc ctgggggcca cggttttaga gggtcgcgac
    36241 ctggggggat ggggagcctt cggggcgtcc ctcccttcgg ggtggtgggc gggcatggcg
    36301 gtggcccttg tcacggggct catggccctg gcggtgctcc gtcgcctggt gatccaggga
    36361 cgctggcggg gctttgcggt ctactgcgcc cttttgggag ggagtaccgt ggtcttttcc
    36421 ctcctgtttc ccggggtgtg accgggggcg tcgcggcttg tgacggcttt cgaaggctag
    36481 acggggaggg aaaggatggc gtcgcgggga atcccttggt ggcttgtgat cctcttggta
    36541 tttttcggca ccgtggtggg ggtgttcctc cagcgtttcg acgggctgga taccctcttt
    36601 cgggacttcg cccgggcggg gtttgacctt cgagaggtcg acctgctctt cgtgcgcttt
    36661 ggcctgtctt ttttcttccg gataaacctc ggtacgctgt tgggaggggt ggtggggctt
    36721 tggcttttat tgagatgagc cccttgcctc cgttggtgct ggcctcggga agcccccggc
    36781 gtcgggccct tctggaggag ctgggatgga gcttcgctgt ggttccgacg gaggtggacg
    36841 agacccccga ggcgggggaa agccccgagg ccgtggcccg gcgtctggcc gaggccaagg
    36901 cggccgccgg ggcccgggct ttccccgggg cgctggtcct gggggcggat accctggtgg
    36961 ccctggggga tcgattcctg ggaaagcctc gacaccggga ggaggcggag tggatgctat
    37021 cctttctcca ggggagggag caccgggtgg tgaccggggt ggctctggaa tggggacccc
    37081 ggaagagcag cgccgtggaa gtcacccggg tgcgctttcg gtccctcgat cccggggcgg
    37141 tgcgggccta tgtcgccacg ggggaggggg acgacaaggc gggagcctac gccattcagg
    37201 ggcgtggagc ccttctggtg gaaggcatcg atgggtgtta cttcaacgtg gtggggcttc
    37261 ctttggggcg cgtcagcgtc cttctggagg gctggggagt gtctttagag cgccagtgga
    37321 ccccgggagg tgagggagct tgagccgacg gattttgttc ttcggaggcc tgggattggc
    37381 ccttttatcg gccctgtgga gcgtcggtca ccgttggggc gccgagttgg agcgccgcca
    37441 ggtggcgttg gtagtggaat accgagatat cgtcgccatg gcggcggaag agcacctgcc
    37501 ccccgaggcg gtgcgggagc gattgcagcg ggcgggactc caaggtgtcg tggtggggga
    37561 gctcacgggg gaagaactat cccagggggc cttgccgttg tggtatggcc ccttggggag
    37621 cctgcccggc gaggcgggat cgaagtccat cgatctcccc cctaatcgag gaaccctatg
    37681 gctctccggc cgatctggtg ccttggcttc agcggtggaa aaatacgtgg ccctcaagtt
    37741 ccccgacgcg acggaacgcg gggggcgttc gggaaaggtg tgggtccttc cccagggggt
    37801 gaaagagctc ttcgaagcgg gggtcttccc cgatgtggag gggctgtcct ttgccgaaag
    37861 tcaagggctt cccacgatct accgccccgc tccggccccc ggcgtggctc cggagcgagt
    37921 ggccgccctg gcggacgcgg tgttggcgga ccaccgtcat gtggtctgtt tggccccctc
    37981 gggagcggtg gtggcgggat accccgatct gggggccctg ggcaaggtgc tgaaatcccg
    38041 gggggtggat ctggcccagg tggagttctc cgcccagatc gggggggcgg cgctggagtc
    38101 gggggccttc ccccgccttc ttcccctgca tagcgtgacc caggacgagg tgatctccaa
    38161 acgcctctgg cgcccccttt tggtggatcg tatgatccgg gccgcccagg aacgatccgt
    38221 gcgcctgttg ctcatgcgcc cctaccttct ggagtccggg tctcgcctga cgaagctgga
    38281 gcaggatctg ggaagtgtgg ccgaggccct gcgccgggcg gggtacgccc tcacctggcc
    38341 taccccctac tcccccggag cccaagattt cttcggtgcc tgggcttgcg ccctgggggc
    38401 tctcttcgga ggaatccact tcttgtggcg tttccggggg attcaggatc gggggattcc
    38461 cggggcgagc ttgggggtgc tcctcgtcgg ggccgcgctt ttggccttta tggcgctccg
    38521 gatgcccgga gtggcccggt ggatcggggc ggcggtggcg gtgattaccg tcaccgaggc
    38581 ttctttggaa gccctggagg gatggcgcac cccccttcag gccctggcca aggccctttt
    38641 ggtgctcctg gcggggggaa tggccatcgc ggcgttcttc ggcgtgccgg agtacatgct
    38701 ccgactgaag accttctcgg gggtcaaggc cacgctcctg cttccccccc tgctggtgtt
    38761 gctccacgac tttcgacggc gaatctatcc cgaatccctg aagagcgtcc tctcccgtcc
    38821 ccccctctgg ggggagctgg cgctcctcgg ggggtttttc gtggggttgg caatcctggc
    38881 ggtgcgtagc gacaacgtct ccctggctcc tcagtgggaa atggccctca gaagttcgct
    38941 ggagcagatc ttggtggccc gtccccgaaa caaggaagta tttctcggct atcccgcctt
    39001 ggtgctctgg tatgccgtcc gccgggcgga tctatcccct cgttaccggg aggtactacg
    39061 cctttgcgcg gtgttcgcct tcgcctcggc cctgaatagc ttctgtcact tccacacccc
    39121 cctgtacttc acgctctggc gggtgggcaa tggatttttg ggagggctct tggtgggggg
    39181 ccttttggtg gctgtggggc gatggatcat ccttcccctg tggcgacgcc tggccccggc
    39241 gatcatggca tgacctcccg atcctgggcc gccgcgctct gcggctacta tggctttgga
    39301 aattttgggg acgagctctt ggccgaggcc ttggtccgag ggatggaaga gtgcggggtg
    39361 ccccgggaac gcctggtgat tttgagtgcg gccccggaaa ggagtgcccg ggatctggga
    39421 gtggcctccg tggatcggtg gagtttccgg gaggtctggg gagcgctgaa acggtcggaa
    39481 accctgcttc tggggggcgg gggactcttt caggatgtca ccagccttcg atccgtccta
    39541 tactatgcgg gagtggtgca cctcgcccgc atggccgggt gccgtccctg ggccttcggc
    39601 cagtccgtgg gccccttgaa gagctggata ggacgggcct tggcgcgccg ttccctgggg
    39661 cgctgtgcct ttcgcggagt gcgggacgcc ccctcggagg ccctattgcg ccgatgggag
    39721 atggagtgtc gccgttcccc cgatggggcc tttcttttag cggacgaggg taacccgtcg
    39781 ccagcggaga cggggaaaga ccttttggtc aacctgcgcc cctggggcgg aagcctttcc
    39841 cgggactgcg ccaagatcgc cgcagtggag gcctctcggc gtcggctctc cctggtgggg
    39901 gtggccctgg cccccgaaga cgaaggggtt ttagaagacc tcagacgcga cggcgttttt
    39961 ccctgcacca gggtggtgac tttgaccgac cttgtccagg cccgggaact cttcggcggg
    40021 gcggcgatgg cggtgggcat gcggcttcac ttcctggtga tttccgccgt gatgcgtcgc
    40081 cccgctgtgg cggtgcccta cgaccccaag gtggaatcct tcgcccggga ttggagtttt
    40141 cccgtctggt acggtgaggg gcctctcccc gagcctgggg gggcggaaaa agaccccggc
    40201 gatgcccggc aagaagtgaa ggcggccctg gagagcgcgt ggctggcgag ctcgcaggaa
    40261 gcgaggcggc gggaggtgga ggcgtgacga gcgctccgag cgcggcggaa acggcccggg
    40321 aaatcaggaa agacgtggtc cgaatgattt ccctgacggg aaggggaggg ctggggattt
    40381 cccttgccct ggcggacctt ttgacgtggc tttactggtc ctaccttcgc gtcgaccccc
    40441 ggaacccccg gggagaagac cgggatcgcc tggtcctggg caccggggcg ggggccccgg
    40501 ccctctacgc caccttggcc cgacgggggt acttcagccg ggaagagctg tggaacttct
    40561 cccgcctggg ggccattctg cagggggttc ccgccaggct ttccatcccg ggggtggatg
    40621 cccccctggc ggagcgggga cgggccctgg gggtggggtg tggcatggcc ctgggccttc
    40681 gccgcaaggg aagccctcgc cgcgtggtgt gcctgctgga cggggcggcg tggcaggaag
    40741 ggcttttctg ggaaggcgtt cggcgatgcg gggcctgggg gttgggtgga gtcacggcgg
    40801 tggtggcctc ctcgtcggag gggatctcct cggcgtggtc cccatcggag gcggaaagcg
    40861 ccttgatcca aagggggtgg cgcgtccttc ggacgacggg gcactgcgtg gatgtcctga
    40921 gccgcgaggt ggaagacatg gaacgaaaga ggacttcgga gcccctggcg gtgctggcgg
    40981 aaaccaccct gggacgggga gtacgtctgg aaggggagcg ggacgagcgt tttcaccacg
    41041 aaggacgtcc ctgggaccgg ggcgtcttag gggagacgtt gcgggagatc gacggcaaaa
    41101 aagaggggga ttccggtgct ccattcgttt tctgacgcct ccggagccgt ggagaggacc
    41161 tttgtcgaag cgttccggcg ggacgaacga tggatgatgg tctcatcttc ctccgccgac
    41221 gccctttccc gggcgatttt tcgagagttc ccggagcggt tcctggccgt ggacggcgag
    41281 gaaagatcgg tggtgctcac cgccgcaggc cttgccttgg aaggctttct ccccatggta
    41341 gggccggggt cgccccgtat agtgacccgg gcgtacgacg agattcgcgc cgccgtggcc
    41401 cttccatccc tgccggtgct ccttttgggg agcgggggag ggctttttcg aggagatctg
    41461 ggggcccccg atcaggtggt ggaagacctg gccctcatga ggacactgcc gggcatgagg
    41521 gtctttttcc cctcggacgc atcggttttg gccgaaatcc tggcggggcc ccacgacggc
    41581 cccgtgtacc tccggtggga accctggggg ggggattctt cggggtccgg aggaacctct
    41641 ccccgggaag acctcctggc gggggggcgg atcaggcgcc ccggaagcgc ggtgacgatc
    41701 tgtacctacg gtgccctggt gcgtgaagcc ctccgggcgg cggaaatcct ctcttctcag
    41761 gggatcgatg cggaagtgat agaatgtctc tcgttcaagc cccttccaga aaacctgatc
    41821 ctcgcctcgg gtagaaggac ggggtgcggc gtggtggcgg aagagcacgt gacggagggc
    41881 ggtctggggg ctgcggtggc tgagctcatg gggtgctatc cgatccccgt gctctcggtg
    41941 tccctggagg gccactttgg ccaaagtggc accgaggcgg aactccggga gtactacgcc
    42001 ctcacccggc agcacatggt gggggcggcg gtgcaggcct ggagcatgag gaggggcggt
    42061 tgatggatat ccgccttcag ggggtctcca agatctttcc cccggacatt cgcgccctgg
    42121 aggacgtgta cctatccatc ccccaggggg agtttgtcta cctcgtgggc accaccggat
    42181 cggggaagac cacccttttg cgcctcattt cccgggaggc ccagatcacc cgggggcaga
    42241 tctatgcggg atcttataat ttgaaaaaga tccccacgct gagcctggcg atctaccgtc
    42301 gggacatcgg ggtggttttt caggatttca agctcctccc cgacctcacg gtcatggaga
    42361 acgtggcctt cgtgttggaa gtcatcggcg tgcctccccg ggagatttcc gagcgggtgg
    42421 gggaggtgtt ggagcgggtg gggatgtggc gccgccgctt tctcaggcct cctcagcttt
    42481 ccggaggaga gcagcagcgg gtggccatag cccgggcggt gatcaactcc cccgccgtct
    42541 tgctggcgga cgagcccacg ggcaacctgg acacccgcac cgccgaggac atcatgcagc
    42601 ttcttctgtc catcaacgcc tcggggagca ccgtgatcat ggccacccac aacaaatacc
    42661 tggtggatgc ctaccgccag cgggtggtgg agctccgggc cggtcggatc gtccgggacg
    42721 aaaagcgggg gaggtacgac gtcgatgggg acttttagat atgccctgag ggacgccttt
    42781 cgccttttgg gacgccactg gggcttggcc ctgctcacgt tggtcaccgc cacggcggtc
    42841 ttttttctcg tgggggggag tgcccttctg gtggtgaata ccagccgcct ggtggctcgg
    42901 gtggagggag aactggtgat ccacgctttt ttaaaaagcg ataaaaacat ggaggccctg
    42961 cgggaaaagg tgcggcagta tcccggagtg gcggaggtcc gggaggtgac ccccgccgag
    43021 gccatggacc gcctgaaaaa atcgatacaa aatcaggccc aggccttgga gatcctggga
    43081 gaaaaccccc ttccccccag tttggaaatc cgcgtggagc gggcccagga cgtgccgggg
    43141 gtggcccggg atctgctgac cctgtcttct gtggacgacg tggtctatcc cggcgcactg
    43201 gcttcccggc tggcccgggt ctcccgggtg gtggtggagt tttccctggt gatcttgatt
    43261 atcgccgtca ccgccagtgc cctggtactc tttaacacca tacgcatcgc ggtgtactca
    43321 aaaaaagaag agatctcggt gatgctcctg gtgggggcca cgccgagttt tgtggccatg
    43381 cccttcgtgc tccagggaat gattctcgga gtggcggggg cggcgggggc tctggggctc
    43441 ctgggagggg cctaccgggc cctggtgggg ggcgtggaga cctcggtgcc ctttctcgag
    43501 atggtaaaag accctgacct cctggcgcgc ctggggctca tcctggtggg agtggggatg
    43561 acggtgggat gggtgagcag ttggcttgcg gtggaccgct tcattcgccg ggcccttcgc
    43621 ccgatttagc ccgtgagggg agtggccatg gctttcaccg ccgagggttc gagaaaagcc
    43681 gaccgagtaa ggatgatttg cgccagggtc gtggtcctgg gggccctttt cctgggggcc
    43741 cttcccgtag gggctttgcg gggccaggcc tggggggccg ccagcgccga cctcaaagct
    43801 gacgatgcgg ccagcgccga agacctcgaa aagcgcctcc ggacggaaga aaagcgcctg
    43861 gccgacatcc agaagcgcct gaagttccac aagggtcagg tttccgtgac ccagaaaaag
    43921 gaaaagttga ccctttccga actctccaag ctcacggacg agatcgggga agcccagcag
    43981 cagatcaacg tgctggagct caggacccgg cagacaaaaa ataagctggc ggaggtggag
    44041 gcggagatcc gagccacgga ggatcagatc ggagccattc ggggcaccct ggcggcgcgg
    44101 ctggtggcca tgtataagta cggcgacggg gccaagatcg acatgctttt gggggctcag
    44161 gacgcccagg atgccctttt gaccaccttt tgcctcaatc gcatgaccga ccaagaccga
    44221 aggctctttg cctcggcggt ccaggcccaa cagcgcctgg aagaggggcg ggccaagctc
    44281 ttgggacaca agacccagct ggcgatccaa caggatcgca tgagacagga gcgtcaacgg
    44341 ttggggcagg gcatagccct gaggaacgcc gccctggagg acctgcgaag ccagaaggcc
    44401 aagcacgcca cgatggcccg tgatttggcc cggctagagg aagaaatcga gcgcatcatc
    44461 cgcaccctct tgcaggagcg ccagcgtatg gcagaaaaat cccgcaagga ccgggcggac
    44521 cagacggggg acaagaccct tctcctttcg gctccctcgg gaccattgat gtggccccat
    44581 cgggggaaga tcaccagccc ctttggcagc cgcatccatc ccattttcaa gacccgcacg
    44641 gtgcacacgg ggatcgacat cgatggaaac atgggagacc cggtgaaggc ggcggcggac
    44701 ggagaagtgc tctatgcggg gtggctcaaa ggctatggtc agatcgtcat cctggaccac
    44761 ggagggagcc tttccacggt gtatgcccac atgtctcgca tcaacgtgga agaaggccag
    44821 cgggtgcggc ggggacatgt ggtgggacgg gtgggaagta cgggaacttc cacggggtcc
    44881 catcttcact tcgaggtgag ggtggatggt aacgccaaag aacccctgcg ctaccttagc
    44941 cgttgatggg atcctaaggg taaaagaggg ggcgaagggc cccacgggac tcgactttca
    45001 tggagtgaag aagcgagcgc ggaggaagaa aggtgtgcga gaggcatggg gaagcggtgg
    45061 cgtgatgtga ttttgggggt tttcctggga gtggcgctgg cgggaggggt ggtggcgtac
    45121 gccgccgatg tggacctggg gcagatcgtc cccttccggg cccagtccct ttggctgatc
    45181 aagcaagccc gggccatact ggagacttac tatgtcgacg ccgaagaacc ggtgtcggag
    45241 gatgtgctgg tgcggggggc cctgaggggc atggtctcct ccctgggaga cccgtacacc
    45301 cgattcaccg atccaagaca gctccacgag gaagagatcg agatgcaagg ggaatacggc
    45361 gggctgggga tctacatcgg ccagcgcgac ggggccaccc tggtaatcag ccccatcgac
    45421 ggcactcccg ccgaccgggc gggattgaaa cccaaagaca tgatagtcaa aatcggcgac
    45481 acctatatcc tggattggga ccaggaaaag gtggtgagcc atcttcgggg agagccggga
    45541 accaaggtca ccatctgggt gcgtcgggaa ggggaagacg agctccttcg cttcgacctc
    45601 gtccgggaga tcatcaacat caaggcggtg cgttttgaga tcctctccaa agacctggga
    45661 tacatcaggc tgaaccactt caaccagaag tgcgtggcgg agatgcggga ggccctggag
    45721 aaactcaagg cccagaaggc ccgggggctt atcttcgacc tgcgcaacaa ccccggtgga
    45781 ttgctcgatg cggcggtgga tatctccgac atgttcctcg acaaggggat catcgtgggg
    45841 atgaagggcc gggtccccaa ggccacggat cagatcttcg ccacaggggg taccctctgg
    45901 gacaaacccg tggtggtact catcaacgaa ggaagcgcca gcgcttcgga gatcgtctcc
    45961 ggggcactga aagaccatcg ccgggccgtg gtgatggggg ccaagagctt cggcaaggga
    46021 tcggtgcaga ccctttttcc ccttcaggac ggctcaggac tatatgtcac catcgccaag
    46081 taccatactc cctcggggtt tgtcatcgac cacgtgggcc tttcgccgga catcgtcgtg
    46141 gaagggacca tggatcggga gctcagcaaa gaccagcagc ttcgagcggc ggaaaaagag
    46201 ctgcggcgca tcctgacggt tcctcgcccc taggggccct ttggcagggg tgattaatgc
    46261 gcgggtatct gaaaaaacga aaagagcgac tttatgcctg ggcggtggtg atttttatct
    46321 tttccctggg gggcaccctt ttgggcatgg cccagtggcg tcgagcccat ctggaccact
    46381 acgacgactg ggagaaatcc atccacgaag cggtggagat gggggaagaa gagaccctca
    46441 tcaccctcat cgccagcgac gatctgggag atcttctgtc gtcccagcga gcggggtggg
    46501 tactggagcc ctctctgctg gaggagggaa ataagcacct ttccggagag gtgccttacc
    46561 gctacgcccg ggagatatcc tgcgaccttc cccgggtggc catcgtggtg gacgacgtgg
    46621 gttacaacgc cgccatggcc cggaagctag ctaaaatccc catacccctc acctgggcca
    46681 ttatccccgg gctgaagggc acccgtcctt gcagcaaggt ggccgacgag gcgaacatcc
    46741 cctacatggt gcacatgccc atgcaggcca cgggggacaa aaaggagggg cgttggtatg
    46801 accttagttg gatcgtatcg gggcaaagcg aagcggcggt gcaaaaggcg gtggcacggg
    46861 ccctggaaga gctccccgat gccgtgggca tgaacaacca ccggggatcc ctttccacca
    46921 tggacgaggc cctcatggtg cccttcgtga aggccctggg agaaagacgg ctgggcttta
    46981 tcgacagccg gaccatccgg ggctccgtgg cctacaaaac cgccagacat cacggcgtcc
    47041 ctgcggcgta taacagcgtg tttatcgatc actataacga cgaaaaattc atgaaagaac
    47101 aactggatcg actggtgatg ttggccaagc gccacggatg ggcggtgggc atctgtcaca
    47161 tccggtcggg gaccttgaag atgttggccc aattggacgt ggcgcaattt ggaggggtgc
    47221 gtttcgtcac cgtgccggag ctattctcgt gcctgggtcg tccgtagggg atcgcaggct
    47281 tgccgtcata aggaggagac aagcatgaag ggacgagtgg aagtcatcgt cggatcccag
    47341 tggggagacg aaggaaaggg gcgagtggtg gacgccctgg cggatcgagt ggaagtggtg
    47401 gcccgttacc aagggggggc caatgccggg cacacggtca tcgtgggatc ggaaaaattc
    47461 gtattccacc ttcttccctc ggggatgctc tacaccggga aggtgtgcgt catcgggggc
    47521 ggtgtggtgg tggatccgga gcagctcctg gccgaactgg ccgatctgca ggccaaaggg
    47581 agggatcggg cccgtctggt catctccggc acggcccatg tggtgatgcc ctatcataag
    47641 ctcctggacg aggcggagga aggccagcgg agcagtggca agaaaatcgg caccaccaaa
    47701 cggggcattg gcccatgcta catggataag atcagccgag tgggtatccg catggaggac
    47761 ttgctcctcc ccgatgttct tcgagacaag ctgacgttca acctcgaggg caaaaaccgc
    47821 ctgctcaccc ggatgtacga ccggcgtccc ctggccttcg acgagatcta tgaaaaggcc
    47881 ctgggatggg gaaagtgcct ggccccctac gtgggggacg catccttggt gatcgccgaa
    47941 gccttggacg aaggcaaggg ggtgctgctg gagggagccc agggcacgct tttggacgtg
    48001 gaccacggca cctacccctt cgtcacctcg tcaaaccccg tggcctcggg ggggtgcacc
    48061 ggtacgggga taggacctcg aaaggtcgat agggtgattg gcgtcaccaa ggcctactgt
    48121 acccgggtgg gagagggccc ctttcccacg gaggacctgg gcccgacggg aaattacctc
    48181 cgggaaaagg gaggggagtt cggtgccacc acggggcgcc cccggcgctg tggctggatg
    48241 gacatggtgg ccctgcgcta tgcggtgcaa gttaatggca tggatgccat cgccctcacc
    48301 aaactggatg tcctcacggg aatgaaagaa atactggtgg cggagtcgta cgagatcgac
    48361 ggggtatcca cggatcgctt ctccggggcg gcgcaccgcc tggtccaggc cactccagtg
    48421 tatcgcgccc tccccggatg gcaggaagat ctttcctcct gccgccgctt cgacgagctc
    48481 cctcaggcgg cccagaatta cgtcacctac ctggaggaag ccacaggtat ccccgtggtg
    48541 ctcatcggag tgggtcccgc ccgggaagcc accatcctgc gggggctcta gggatcgatt
    48601 gaccccaggg cctccggggg acctcccatg gtgcttgccg atatcgactt ctgcgcgcta
    48661 aaggacgtgg atgccctgga ggccctggaa cacctctctt tttcccatcc ctggggtcgg
    48721 gggatttttg ccgccgacct gggaagggat cgtcctgggg cgctctacct gggggcccgt
    48781 ctcaaggggg agttcgtggg atacggggct tgttcctgga cgggggatcg gttgcatatc
    48841 ctccgcttgg cggtgcaccc cgacgtccgg cgctttggcg tggggagcca gctcctggtg
    48901 gccctggggg agatcggcgc ggcggtaccc tgtcggaggg cctttttaga gctccggcga
    48961 tccaatgtgg ccgccgcaac gctctatgcc cactttgggt tcgtcgaggt gggatttaaa
    49021 aagagatact acagcgataa cggagaggac gccctcattt tggaagcccc cttgcccctc
    49081 cgagaggtcc ccccgaaaat cccttagggc ctgaaagagg agcgggcgat tcgggggaag
    49141 ggcctcggag ggcgaaggct ttcgggatct ttcgcgagac gcgaatcccc agatcggcaa
    49201 taagttttcc accgctgggg ggtggggttt tccccatcct cctttgtgcg ccgggggttc
    49261 ccctggcatg cccactccct ccccactccc tccgatagaa ccggccatct gccgatggtg
    49321 ggcgtgtttt agacggggcc gggggcggga acgacgtcgc gacggcggtg ggagaggctt
    49381 ttgtttattg ccaaagcgac gaatgtgccc acgtcctcga cgccacacgg ggaagaagat
    49441 gataaagtag ttaatttttt tgttttggag tccccgtggg gtctggagaa aaaaaattcg
    49501 cgggaaaaac ccgcgaaccc ttgcaatcta tggtgggcga cacagggttc gaacctgtga
    49561 cctcttccgt gtgagggaag cgttcttccg ctgaactagt cgcccacaac ggaaggaatt
    49621 ataccctccc tctccgagcc ccgtcaagcc ctatatcgca tatgaccttc tcgggctgga
    49681 atcggccttt ccgcgccctc ggccccttgc ccggacctcc tagttttttt ctattcaggc
    49741 cgcgcccgcc ccttctccca gagatttcgg gccctatgat ggcaggggca agggaagagc
    49801 cttgacggcg aagtgataga gggagtagca tgggatcggt ctttcccctc gttctcacgc
    49861 ctgacatttt tcgcaggaca gagttgtgtc ggatgatatt tccggtgtca tggccttcta
    49921 aaagagaaga tcgcctcctg gtgtgccagg gtaggggtgg aggcgatcaa aaagggtccg
    49981 taccctaggc aagggggccg gaagggagta cgatgtccgg gagtaagttc cggagccttc
    50041 cgggcggata gagggcgacg gggagggggt catgaaaatc tcgccgtggg acgacgtgcc
    50101 ggagtactgg gctcattggg gatttgaacc ctggagccat ggccaccttc gaggggtccg
    50161 taggaaggtg atcatgcgca agggggggct tttgggggac gtggcccgct acgccgtgtg
    50221 ggatcacatc ctctggggac accgggggag ggagagcgcc gaagagatcc tccgggaggc
    50281 gagtcccttg cccgaggtga tgacccagcg ctacgtgttg gtgggggacg aagaggaccc
    50341 ttttcggcta cgctcttttt ggtgggggtt tcggggtttc gtggaagtgc tgtgctaccg
    50401 ccccagaggg cggggtcatc ggaagtttgc caacctcacg gggctggtag atgcgctgga
    50461 aggctacgta atccggcgaa aaggggaggt gacgggaacc aaaggcgacg ggacggagag
    50521 cgacggggtc ggggtgtcat gatattattt ttaggaggtg ctttcctgtg ctgatgacga
    50581 tgtttatcgt gacggtggtc ttttttctgg cggcctacgt gctctatggg cgcttccagg
    50641 tgaagctcta cggccttgac gacagccgga aaacccccgc cgaggtctat ttcgacggca
    50701 tagactacgt tcctacccat cctgcggtgc ttttggggca tcacttttcc tccattgccg
    50761 gagcgggacc cattgtgggc cccatcacgg cggcgtccat gttcgggtgg ctccccgcct
    50821 acctttggtg tctcataggg tcggccttcc tgggaggcgt gcacgacatg ggctccctgg
    50881 tggcatccat caggcataaa gggctctccg tgggcgaggt ggtggagcgc tggatcggcc
    50941 atcggggtaa gaaacttttt ctgactttca cctggctcgc cctgatactg gtcgtggcgg
    51001 tctttctcga gctggcggcg gggaccttcg ccaaagatcc ggcggtggcg ttctccggca
    51061 cgctgtacat gctgctggcg atcatcttcg gcgtgctgat ctaccgcaag ggactctctc
    51121 tgctctggtg caccctcatc atgctcccca tcgtcatcgg ggcggtgttc tacggcagca
    51181 gctccccctg ggtggcccaa ctcttctcct tcggctacga cgtggccacc aaggaggggc
    51241 aggcgtccct gctggatctc tggcgcatta ttctcatcgc gtacatcttc ctggcgtcgg
    51301 tgctccctgt gtggctcctt cttcagcccc gggactattt ggcctcctat ctgctctact
    51361 tctccgtgct catcggcgct gtggggatga tcttcggcaa gagcttcgag cccgtgctcc
    51421 cggcctttat gggattttcc aacgccaagg gggaattcat ctggcccctc ctcttcgtgg
    51481 tcatcgcctg cggcgcaatc tcgggattcc acgccctggt gggaagcggc accacggcga
    51541 agcagctcaa gaaagagagc gacaccctgc tgatcggcta cggcggcatg ctcatcgagg
    51601 gcgtggtggc ggtcatcgcc ctgggcacca tcatcatcgc ggggctcccc gccgacggca
    51661 gcgccgtgaa ccccatggcg atctacgggg cgggcattgg caagttcgcg tcccttttgg
    51721 gcatcgacct caaagtgggg gcgtctctgg gccttctggc gctgaactct tttatcctga
    51781 cttccctcga tacggccacc cgcctggccc gctaccagtt ccaggagttc tttgacatga
    51841 aggtggatcg ctacaccgcc acggcggtgg gcgtggcggc ggccctcttc ctggtgttct
    51901 tccggaccgg gggcaaggcc gcctggaccc tcatatggcc cgtcttcggt gcttcgaacc
    51961 agttggtggc cgctctgacc ttgctggcgg tgggggtgtg ggtgaaacac ggcctgggca
    52021 agagcgccac gttccttctg gcccccatgg ccttcatgtt tgtcaccacc gtggcggcct
    52081 tggtgctcct catcaaggcc aacctgggta accccctgct ggcgggcatt tccgccgtgc
    52141 tcctggtgct ggcggttttg ctggtcaaag agggttgggt ggcgttgcag cggaagacgg
    52201 gggaacctct taaagggtaa gtccccgaag gagggccgcc cgtagagccc gaatcgcccg
    52261 ggatcggacg gtggtccgcc cccgggcgat tcgggctcta tttttaggta tctgggctag
    52321 agcgatgaac gggcttcggc caagcgccgg agggcttcca tcaaggtggt ccgggggcag
    52381 gcgaagttga gccgggcaaa gccatcgccc cccgggccga agctccggcc gtcgttgagg
    52441 ccgaggtggg cccgatgaac gaggaaggtc cggagctctt ctccgcagag aaggagggga
    52501 cggaagtcga gccacgccag gtaggtcccc tcggggaggg agtaccccac ttcgggaact
    52561 gtgcgggcca gctcctcttg tatgaggcga cgatgcccgt ccagataggt catgagggcg
    52621 tcgagccagg gccctcccgc cgtgtaggcc gcctcggtgg cggcgatgcc gaggacgttc
    52681 cacatctcca ggtcgagggc ttccagcgtc gacttcatcc ggcggcgtac cgtttcgtct
    52741 tggatcaccg ccatccccga ccggagcccg gggatgttaa aggtctttcc cggggaggtg
    52801 aacgtgaggg cgatgcgggc cgcatcgcct cccagcgagg caaaggggat gtggcggtgg
    52861 ggggaaaaga gcaggtcttg gtggatttcg tccgaaagga ccaggatgcc gtgacgaagg
    52921 caaagggctc caagggattc tagttcttgg cgttcccaga cccgccccac ggggttgtga
    52981 gggctggaaa ggatcagcag gcgggtggcg ggggtgatcc ggcgctccag gtcctccaga
    53041 tccatgacat agcgcttgcc gtcccagcgc agggggtttt ccaccacctc gcgcccctgg
    53101 cgctccacca cgctcctgaa gggggggtat acggggggct ggatcaccac cccatctccg
    53161 ggggtcgtga aggtctgcac cgccagggcc agcagaggga ccaccccggg ggcctctaaa
    53221 atccagtcgg ggtccacggc ccatccgtgg cgctgggcca tccatgcggc gaaggcatta
    53281 tgggctcctt gggcccggag gggatagccg aagatgccgt gatcgacccg ggaccttagg
    53341 gcctccatca ccgcctccgg agcggcgaag tccatgtcgg ccacccaaag gggtaacacg
    53401 tcctccgtgc caaagacctc ccttcgcccg tcccacttgg cgcaggccgt accttcccgg
    53461 cggtgccgta gatcgaaatc ggggttcata aaacggtcac ttcccttcac aggatcgccg
    53521 tgcgggaaaa gcccgtcggt tcacggcgaa agccgccgca gacggcaaaa aaaggcacaa
    53581 aaccaaaacg accggtccgc cggagcccta ggcgggcccc atcggccgta aagcgattgt
    53641 gccaccgacg gccctcatgc tacgatacgg ggtagttttt gtcaaacgtg aagggaccct
    53701 gacggggcac gggggagagg agtggggcga ggatggcgaa agtcgtggtg gtgggctccc
    53761 tgaatatgga cctggtgatg cggacggcgc gcttgcccca gatgggggag acgtggctcg
    53821 ggggagcatt ctccaccgcc gagggaggaa aaggcgccaa tcaggcggtg gcatgcgccc
    53881 gtatgggggc ttcggtggcc ctggtgggtc gagtggggcg ggacgatttt ggtcgtcggc
    53941 tcgtcgaagc cctggtcgtc gagggggtag acgtcggggg ggtccgtttc gacgacgaac
    54001 accccacggg ggtggcgtcg atttatgtgg tggaaggcga caacggcatc atcgtggctt
    54061 ccggggcgaa tgctgcggtg tcggcgcggg atgtgcgcga agcgcggtcg gctttcgagg
    54121 gggcccactg cgccctcttc caactagaga cgccgctgga ggcgctgggc gaggggctta
    54181 gggtggcccg agaggcgggg gcgatgacgc ttttgaaccc cgccccctgg catgcgctgc
    54241 ccggggactt ctttcccttc gtcgatgtct tcatccccaa cggcatcgag ctcgggcagt
    54301 tcgcggggac cgccgatctc gaggcggggg cgacgaaggc ccttgaagca ggagtggggg
    54361 cggtggtggt aacggtgggg agcgaggggg cctggctatt tcgccccggc gtcaaagagc
    54421 atgtggcggc gccgcgcgtc gaggcggtgg atagtaccgg ggcgggggat gcctttgtgg
    54481 gatcgctggc ggtgtcgctg gccgaggggc tttcgcttcg cgaatcgatt tcctttgcgg
    54541 tatgtgccgg agcgctggcc tgtacgcggc tgggggcgca gcccggcttg ccgcgccgga
    54601 aagaggtgca cgccctgcgg cgcggatggg cagggggcga gggaggaatg agatcatgat
    54661 gcgacggggg attttgcatc ccgagttggc ggctctcctg gccgcctgtg gacacttgga
    54721 cgagatctgt gtggccgacg ccggtcttcc catccccccc gagatgcccc gggtggacct
    54781 ggcctatgcg ccggggcgag cgcccttcat cgacgtggtc gaagttattc tgggggagat
    54841 ggtctttgag agagccttgg tggcggagga ggcggcgact cggagccccg agatgcttgc
    54901 ccgtctgcgg gggatcctgg gcgcggtggc gtgcgaatct gcctcccacg aagagttcaa
    54961 aaatagggtg actcgggctc ggctggtggt gcgtaccggc gagttcaccc cttatgccaa
    55021 tgtgctcttg cgctgcggcg ttcccttcta acgacccatg atcatgcggc gcaccccctc
    55081 ctcatcccgt cttccggtgt tgttggcgat cctcgtgggg atcgtggccc tgctgggctg
    55141 gcatgaaccg cgctttatcg aaataaacaa cctaattaac atccttcgcc aatcttccat
    55201 ggtggggctc attgccctgg gcatgacctt ggtgattctc accggtggca tcgatctgtc
    55261 ggtgggcagc ctggtggcgg tgtccgccct ggcgggagcg ggggcggcca gggccggggt
    55321 gacctttccg ctctgttggg gggcggcgct ggccgccggg gcgttgctcg ggatggttaa
    55381 cggcctgggc gtggcgcgac ttcgccttcc cccttttatc atgacccttg ggatgatggg
    55441 cttcgcccgg ggtctcgcct ttatggtcac cggggggaag cccttgacgg gcttcgatgc
    55501 ctcttttcga tggcttggga tcggtgggtg gatgggcctt cccctcccta tttgggtttt
    55561 tggtggtttt ttcctggtgg tgtgggtgct tctggagctc atgccctggg gggatcaggt
    55621 gagaagcgtg gggtctagcc cccgggcggc ctggggcagc ggcgtcaacg tccccggcgt
    55681 gacggcccaa gtctacgtgc tctgcggtgt ggcgtcggcg ctggtggggt tgctgctggc
    55741 gggtcgtatc gactccgcgc agcccacggc gggcgtgggt tacgagttcg gggccatcgc
    55801 cggggtggtt tttgggggta cgagcttctc cggcggtcga gggtcgctcg tgggcacggt
    55861 gatcggcgtg ctgatcatga cggtgttgga gagtgggatg aacatgttga acgtgaatcc
    55921 ctttgcggag caggtggtaa agggggtggt catcgctgcg gcgctggtgc tctatggacg
    55981 cacggggcag gcgctacggg gttaggggag gagatcgcgg agggcgcaac gttcggagtt
    56041 gttcagcgat ggcggcggcg gcgaagaggg cggcaggacc gctcgggtaa aaacaggagg
    56101 tgcgtttaag tgaagaagat gatggcggca tttgcggtgt tgtggctgat ggcggttccg
    56161 gccttggcag atcagccggt gctcggcctc gtggtttcga ccctcaacaa cccctttttc
    56221 gtcagcctga agcagggggc ggaggaagcg gcggcgaagg cccaagtgtc gctggtggta
    56281 ctcgatgcgc aggacgactc ggccaagatg acagcggcca tcgaggactt gatccagaaa
    56341 aaggtggccg ttatcctcgt gaatcccacg gactctgacg cggtaactcc gtccatcatg
    56401 aaggccaacg aagcgggcat tcccatcttt accatcgatc gcggcgcatc ggggggtaag
    56461 gtggtctgcc acatcgccag cgacaatgtg gcggggggca agatggcggc ggagttccta
    56521 gccgaggcca tcggccataa gggtaacgtg gtcgaattga tgggcatccc cggcacgtcg
    56581 gcggcgcgcg atcgcggcaa gggctttaac gatgccatcg cggccttcgc cgacgtcaag
    56641 atcgtcgccg ctcaggcggc ggatttcaac cgcgacaagg ggttgaaggt cttcgagaac
    56701 attttgcagg cgcagcccga aatcacgggg gtctttgcac acaacgacga gatgatcctc
    56761 ggtgcggtgg cggcggcgga agcggcgggg cgtacgggca tcgtcttcgt gggattcgac
    56821 gcggtcgatg acgcggtgaa ggcggtgcag gagggaaagc tggcggccac cgtggcgcag
    56881 cagcccgcgc tcatgggatc cacgggcgtg gagaaggccg tggccttcct cggcggagca
    56941 tcgcttcccg agttcatccc cgtcgacctg aagcttgtgg tgaaggaata gaaaaaagat
    57001 gggtggggtg gccttcgggc ggccccaccc cgggtgttag atagggcggc ggtcatcctt
    57061 tttccgtgga ggaatgtcat gagcgcactg ctggagatga ggaatgtcca caaacgctac
    57121 cccggtgttc acgcattgaa agggatttct ttctctctgg aggagggaga gttccacgcg
    57181 ctggtgggcg aaaatggggc gggcaaatcg accctcatca agacgttggg cggcgtggtg
    57241 ccccccgacg aggggggcga gatcctcgtg cgcggcgagc gggtggcctg gagtggcccg
    57301 ggggatgccc tccgggcggg ggtgggcatc atccatcagg agcttagcct cgcgccgcac
    57361 ctgtccgtgg cgcagaacat ctttctaggc cgggagctac gccggggggt ttttgtcgac
    57421 tctcgccgta tgcgggagat ggcaaaagac ctgctggccc acctgcggtg cgacatcgac
    57481 cccgatagga ctgcggggga gctctccacc ggggagcagc agctggtgga aatcgccaag
    57541 gccctatcgc agaaggtctc catactggcc ttggacgaac ccacgtcgag cctttcggcc
    57601 cgggagacgg aaaatctctt tcgggtcatc gacgatctga agagccgggg ggtggggatc
    57661 atatacattt cccaccggct agaggagatc tttgcccacg cggaccgcgt gaccgtactc
    57721 cgcgacggcg aggtgaccgg gaggggagcc atcggggact tcgacccttc aagcatggtg
    57781 gcctgtatgg tgggccgtcg ggtggaggtg ctctttccgc gaagctttcg agatcccggc
    57841 gaagtgctcc tcgaagtgcg ggggatctcg cggcgcggag tcctgcgcga tgtatccttc
    57901 gagctgcgtg agggggagat cgtgggagtg gcggggctgg tggggagcgg tcggacggaa
    57961 accgcccgat gtctcgtggg gctcgatccc gttgacgggg gcgaggtgaa ccttgtggga
    58021 ggggcgcttg ccctaagggg ccctcgggag acgcttgatc gggggatggt gctggtgccc
    58081 gaggatcgca agcggcaggg gctcttcctc aatcgatcca ttcgcgataa cgccacggtc
    58141 atggagctcc tgcacggcct gcgccggggg ccctttgtcg accgagagaa gcgggacgag
    58201 gcagcccgcc gccatgtgcg gaacttttcg gtgaagtgtc gcgacatctt cgatcccgtt
    58261 ttcgccctct ccggaggaaa tcagcagaag gtggccatcg ccaagggcct ggcggtggcg
    58321 cctcgggtgc tcatcctcga cgaaccgacg cgcggagtgg atgtgggagc ccgggcggag
    58381 atccacgaga tcatgaacga tctggtccat cggggcatgg gaatcctgat gatttcctcc
    58441 gatttgcccg aggttctggg catgagcgat cgggtgttgg tgttccgcca cggaggtatc
    58501 gctgcgtcct tcgatcgggg cgatgcgacg ggcgaaaagg tgatgctggc ggcggcgggg
    58561 ggcggagtcc atggcgggta agtggcaaag gttcggttct ctggcggtgc tgatcgtggt
    58621 ggtggtcctt ctcgcgctgg tcgatcgcga cgtgatcgat ccgaagaacg tggcgaacgt
    58681 gcttgctcag tcgacggtgc tgggggtctg tgccgtcggc atgacctttg tcatcctcac
    58741 tggcggcatc gatctcgccg tggggagcgt gatggccctc tccggggtgg tgggcgccct
    58801 ggccaacacg gcgatggggt tgccctggcc cttggcctgg gctcttggtt tgggggcggg
    58861 gatgatggcc ggagccctct cggcggcgtt gatcgcctgg gggcgcatgt tgccctttat
    58921 agccacgctc tgcgtcatgg ccatagctcg gggaataacg ctgcttatga cccaagggag
    58981 tccggtctcg ggcctttcgc cgggctttac ccatgcgggg tgggcgtcgt ggggtgccgt
    59041 gccgattacg gcgctaatct ttttactgct cgcgacggtg gcctgggtgg tgctggcgaa
    59101 aacgcccttg gggccgcacg tgcgggccca gggagataac ccggaggcgg ctaggttggc
    59161 ggggctctcc gccccggggc tgacctttgc ggtgtacatg acctcggggt tctgtgccgg
    59221 gcttgcgggg cttctgatgg cgggtaggct atggagcgcc caacctgcca tcgggacggg
    59281 catggagctg gatgccattt cggcggtggt gctcggcggt accagcctct tcggcggcgt
    59341 ggggagcatt ggtggcacgg tgaccggcac gctcatcatg ggctttctcg acaatggcct
    59401 gcggttgcag ggagtggcga gctacgtgca gatatcgatc aaaggggcgg tgtttgtggg
    59461 ggcggtcatc ctggacctcg ccatgaaggg acactacagc cgacgaagaa gccgggacgc
    59521 gtaaggcgct ccggcctttt taccgtgggg ggcagtccct gggtcttttc cccacgggac
    59581 ttgacagaat cgacgaaggg gctatcatgt ctgtaaggtt tttagttcga caatcggagg
    59641 gatttctgtg gacgaaagca aaaccatgaa aaatcttggc gagggtttcg cgggagaatc
    59701 catggcgaac cgcaagtacc tggcctttgc ccagcaggcg gaggcggaag ggttccatcg
    59761 ggtggccgtc ctctttcgcg ccacggcgga ggcggagacc atccacgccc tggcccattt
    59821 caaggccatg ggcaaactga aagacacccc ggcgaacctt caggccgcca tcgacgggga
    59881 gacctacgag tttaccaaga tgtaccccga tttccttgtg gacgccgagg ccgagaacaa
    59941 cgccgccgcc atccgctcct tcaagctcgc caaggcggcg gaggaggtgc acgccgagct
    60001 ctacgaaaag gccctgaaga acctagaggg agagaaggcc aaggccgctc ggtttttcct
    60061 atgccccgtc tgcggtcaca tcgccgagga cgaagccccc gaggcctgcc ccatctgtgg
    60121 agcaaaaaag agcgcctata aactgtacga gtaaactgga aaaggcaaag acaagctttc
    60181 tgctcccccc cggaaactcc gggggggagc ttgtggtttc tagggtgcgg cgtccgtttt
    60241 tggaagcagg cgcctcacca ggggcatggg gtcgacctcg gtgaggagca cggcggcgaa
    60301 tatgaaggcg caccccagga tcatctgctc cgaaaggtgt tctcccagta ggaaaacccc
    60361 cgccagggct ccgaagagag actccaggct tagcaatatg gagacgtggg tggagggcgt
    60421 gtatcgctgg gccgtgttct ggatcaggag ggccatcact gtgggaaaga ccacgcagta
    60481 ggccatgctc caaaaccccg tggaagagat cgtcgtgggg aaggtctccc agaggagggc
    60541 cagaaccagg ctcagggcgg cggtggtggc gatctggccg aaggcgagga gaatgggatc
    60601 caagtggggc gcaaagcgtt cgatggccag gaggtgggcg gcgaaaaaga cggcgcaggc
    60661 cagggttagc atgtcgccgt agttgatgac gaaatcgccc tttaggtcca aggtgagaaa
    60721 ggccatgccc acgaggcaga gggccgaggc cccgaaggga cgtatcccgg gaaagactcg
    60781 ttttagagcc caggcaaaaa agggcaccag caccacgtag accgccgtaa gaaaggcctg
    60841 tttgctggcg gtggtgtaga ggagccccac agtctgggtg gcgaacccga ggaacaagaa
    60901 ggtgccgatg atcactcccc ctcgcaggtc cttcccggtg atgcgacgta cccggccggg
    60961 aaagatcaag gcgatgagga gggcggcggc ggcgaagcgg atcgtcaaga gccagaaggg
    61021 gggaaaggac tccagggctc ccttcatggc ggcaaagccc agcccccaga cggcggcgac
    61081 aaaaagcagg ctggcgtccg ccgccagcgt ccgaacattt tccctcatga tctattcctc
    61141 tccctcttag ggcccctctt cgaagggtca tgccccgatc attgcatacc atccccgcca
    61201 tggtaccatg acggccctcc cttgagaacc cgccaaatga cctccccaaa ggccctccgg
    61261 acgtggtaaa cttaccgcgc ttcaagagta cccatattgc tcaaggagga attttgcatg
    61321 atgcgtcgaa gtacgttggc ggcactggcc ctgggttttt cccttctggc ggggggagct
    61381 ttcgcgaaca cctcggggga tgtttcggtg ggcgggacgg tccaggccga tcccgagaag
    61441 gtcctggcgg tggtgggcaa gacggagatc aagcaacagg atgtggatgg catattggcg
    61501 aagctcgatc ctcagcgggc ggcctttttc tccaccgagg acggccggaa aaagctcctg
    61561 gaggagctgg tgaatcggga acttttcctc cagtggggcg tggagagcgg cgtcgataag
    61621 caggaagacg tgcagaaaga gatccggagc aagacggaag acgccatccg gaactacgcc
    61681 ctcaaaggag tcctcgacgc ggtgacggtc acctccgagg acatcaagaa gtactacgac
    61741 gaacaccagg acgagttcaa gacccctgag agcgtccggg cccggcatat cctggtggac
    61801 agcgaagacc aggcgaagca gatcgtcgcg gagctcaagg gagggctgga ctttgccgag
    61861 ggggccaaga agtactccac ctgtccctcc aaggaacagg ggggagatct gggctttttc
    61921 tccgcaggac aaatggtgcc ggaatttgaa aaggccgcct ttgaattggc gaaccccggc
    61981 gatatgagcg aacccgtgaa gactcagttc ggctggcaca tcatcaagct ggaagagaaa
    62041 aaacccgctg gcgtggcccc cctcgaagaa gtgcgggagg ggttgcagca gaaggtcctc
    62101 atggaagccc aaaaggcggc ctacgacaaa aaggtcgaag aactccggca gaaattcggc
    62161 gtcacgatga aataaggccc tttagcgatg gcccaggaat ccccttcggg ggattccttt
    62221 tttttgcggc ctgaagggaa cggagggctt cggcggcggg gtcacgaaaa aggcggaaaa
    62281 ggcggaagaa ggcccggggg ccctcttccg cccgaggggc cttgcggccc tcacggaatc
    62341 gtttgcgggg gaaggaacgg atcagtcgtc caggacgtgc atctggagcg gggggaagcc
    62401 gttgaagttg acggcgcaat agctctgggt gtaggcccct gtggtgagaa tggccacccg
    62461 gtccccttcg gagagggaca gaggcagggc gtacttgcga ttctcgtaga ggacatccat
    62521 gctgtcgcag gtgggtcccg ccaggatggc ttcctccgag gggccttctt tttcggagta
    62581 gatggggtag cggatggatt catccatggt ctcgatgagg ccgttgaact tgcccacgtc
    62641 gaagtagatc caccgattgg ggctgacgcc ggacttccgg gagatgagga ccacctcggt
    62701 gaagatcacc cctgcgtccc cggcgatgaa tcgcccgggc tccaccagga tctccgggaa
    62761 gttgtccccg aagtcctccc tcaagaatcg ggtgatctcc tgggcgtagg tggcggtgga
    62821 ttgtgtgggc gccacgtatt ccgccgggaa cccccctccc aggtttatca tgcccaggga
    62881 aatcccctta tcccggacgg cgtcgaagag gtatcggcag tgggcgatgg cgtggtccca
    62941 ctggccgatg tcgcgctgct gggaccccac gtggaaggag atgccatagg gctggagccc
    63001 caggtcggca gcctggagga tgaggcggta gaccgcgtcg ggatgggcgc cgaacttgcg
    63061 agacaggggc caatccgccc cgctcccgtc ggtgaggatc cggaagaaga cccgggatcc
    63121 cggggcctgt cgggccaatt tttgtaagtc gtggtcggaa tccgtggcga agagtcgaac
    63181 tcctttggag aaggcatagg cgatgtctct ttcttttttg atggtattgc cgtagctcag
    63241 gcggtccggg gtggccccga gacgcagcgc ctggtccatc tcgtaaatcg aggcaaagtc
    63301 gaagcagcat ccccggtcga tgaggagttt tagcacgtcg tcgttcgggt ttgccttgat
    63361 ggcgtagtag atcttggcgt agggcaggga ctggtgaagc tcgtcgtact tggcggcgat
    63421 gcggcgaagg tcgatgacca gacagggagt gtctttgtcc gcgacgaagg ctttgatgcg
    63481 ctcaaaccgt tcccggggca tgtaccgctc caggtcaaag ccgtactgct gctgattcgt
    63541 ttccactcgg aaacctccta tcatccccca aaaaatgtcc ccatgcgtgt gggttcgaag
    63601 cgcgatcagt gtagaccggc ggggcccgat gtgcaagggg ggagggggtg atttttacgg
    63661 tttttcgacg ttctccgccg gagatttttc cccctggaga ctccgggcca aatcttccgg
    63721 agagagcacc accaggaggc tgtcctcctc cggaggaacg gccacggagg tgacaagaac
    63781 gcgcccttcc ccttccggag accggataaa aacccccaag agctcctccc cgggggccat
    63841 gccgacgaca aaggccccat catctcggag gacggggctt tccaccgcct tctcctgggc
    63901 caggcgcgag gggggaaaga tctccactcg ccctaggtcg acggaggccc cctcgggaag
    63961 gaccaaaagt cccgttacct tgcgagggaa gcgcaccaag acggggttcg atgggcgtcc
    64021 atcgtcccgg gtcaccgaga gaggtcccgg ggtcgccgac gctccgtagg gcagacgcag
    64081 attggcattg gcatcgttac tcacggggcg cgacgagccg tcggggtagt ggacggtgaa
    64141 gctcccccgg agatttctcc cccgaagcag aagggtcttc tccccagggt ccgttgtgac
    64201 ccgctgtatc acgggatctc cctcgtccag ggccatcatc cgggcggcct cgggcaaagg
    64261 aggcggggtc ccctcctcat cgaaagaggc gccctcggaa acgtgttttt ggacccaccg
    64321 gcgcccctgg cggtagagct cctcccagga agggctccgc tccaacgaga cagtcaccca
    64381 ctccccggag gtgaaggcgc cgggccaggc gaagcacatc ccctcggggg tctcctcagg
    64441 agtgatgacc cgctggtggc gctctccctg aaggcgaacc tggaggcccc ccggactttc
    64501 cggcaggtca tcccggggca aaaggacctg gtcccccgga cgggcggcgc cgctccaggg
    64561 ggaggacaag tccggacgaa agggggccaa gggaagcgtc tcgttgtggg ggtgcagggc
    64621 cagcccctcc gccgaagctc ctcccagcag aaagaggccc agggcaaggc cgcgaaacca
    64681 aggggtatct atcatat
//
LOCUS       DAOOER010000006        60042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_538_length_60042_cov_8.101022, whole genome shotgun sequence.
ACCESSION   DAOOER010000006 DAOOER010000000
VERSION     DAOOER010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 60042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 60042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60042
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_538_length_60042_cov_8.101022"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..601)
                     /gene="nikR"
                     /locus_tag="PL162_01510"
     CDS             complement(194..601)
                     /gene="nikR"
                     /locus_tag="PL162_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HQF90737.1"
                     /translation="MESVVRFSVSMGQSLLDELDEWIVRKGIPNRSEALRQLVRTFVA
                     DSRWEEGHGDVCGSITVTYAHMAHDACTELTHVQHHFGDVILCASHAHMDEDHCLEVI
                     LVRGGVGRVRRLVEGVSGIKGLRSVQPCLTSLV"
     gene            754..1578
                     /locus_tag="PL162_01515"
     CDS             754..1578
                     /locus_tag="PL162_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HQF90738.1"
                     /translation="MKVVLAQTAPLWGDAKGSGQAVLQSLSQVPPSTRMVLLPELWSC
                     SYDNPRMEHHAKESPGLLDALAAWARDRRSGVLAGSLPWPVDGGMVNRTWLLNDTGSP
                     VGFYDKTHLFPLLDEDRVFLPGSVPFFFDVDGFSWGSAICYDIRFPEYIRALALGGID
                     VLAVVAEWPRSRLLAWRTLVQARAIENQIYVLACNRCGEGGGEVWGGHSMVVAPDGTV
                     LAEGGDGEELLVVDLELRKVRDMRRALPMWDHRRRELYEAVRAMEHPEASSPESSR"
     gene            complement(1570..3246)
                     /locus_tag="PL162_01520"
     CDS             complement(1570..3246)
                     /locus_tag="PL162_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQF90739.1"
                     /translation="MPLALVMDGYVDEPACFGVPPYISPYVRYAFGALALAGLDVTYL
                     TCDTWRRTPLEDLWREAVLVVVISGLTVPGRYRGGSPLTLRELEALSSLPRSGPLVLG
                     GPIRHGYALQGGVRARDLPPLAVDCLCTGDPEAVLAHWGRTGQLVPGLTRTYEELRPW
                     AIAGAALAPQHPDFPWVMAEMELSRGCDRSEGQCSFCTERSPGTHEERPPEDVLEEAE
                     ALDHVGVSAFRLGRCANILGYGGSFTPEGRRPGPERLRALYEGFRERCPRLSVLHTDN
                     ANPLTITRFPDAAAEAMEIIVRHNTAGDGLSFGLENVSARVKNRNHLKVSAEEALFAV
                     RMVNEIGGKPKGPRLLPALLPGLNFLVGLADDGDDSLEENRRFLETLLEEGLLVRRIN
                     IRRCMVFPEAPLASLLERYPSRVRERSYRRWKEWIRNEADPVFLSGVAPVGTVIRNVR
                     VEERRGHMAFGRSLGSYPPLVGIGADDLREGDRRDVAVCSHGGRSLTAVPYPVDLNRC
                     DPITLQTLPGIGKARASRIIQRRPIDTLEELPDLLDAPAAIVEALRPFLS"
     gene            3475..4212
                     /gene="nadE"
                     /locus_tag="PL162_01525"
     CDS             3475..4212
                     /gene="nadE"
                     /locus_tag="PL162_01525"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HQF90740.1"
                     /translation="MASLREAALVRDTVVSWLRERAAACGMAGGIVGLSGGVDSAVLA
                     ALLKIAFGDAMLAVMLPCHSDPQDEEDARLVAEALALPWAKVDLSAPYDALTRALEIE
                     GPLSSLALANVKPRMRMTVLYALARDRRFLVCGASNKDEWVVGYFTKHGDSGVDLLPM
                     GDLVKGEVRALATLLGVPRRIVEKPPTAGLWPGQTDEGEMGVRYDELDEVILTGEGDE
                     AAKNFVARARSQSEHKRHMPPVCPIPS"
     gene            4428..5177
                     /locus_tag="PL162_01530"
     CDS             4428..5177
                     /locus_tag="PL162_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQF90741.1"
                     /translation="MSGHSKWANIKHRKAAQDAKRGAAFQRLLRAIIIAAKEGGGDPA
                     MNMRLKTAIERAKAVSVPSDNIDRAIKRGTGEIEGAVYEEVVYEVYGPGGVALIVEAT
                     TDNKNRTTPEIRALLTRNGGTLGEGGSVAWNFERRGLITVEGGVDPDELLLSVAEAGG
                     DDVEEDGESFTVTTAPAELDGVLEALKKAGYVVVDSEVRFVAKTPVDINAKDMAGKIL
                     KLVDALEEHDDVQSVACNADIPDDILEALAD"
     gene            5177..5701
                     /gene="ruvC"
                     /locus_tag="PL162_01535"
     CDS             5177..5701
                     /gene="ruvC"
                     /locus_tag="PL162_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HQF90742.1"
                     /translation="MPLRCLGIDPGLGRLGFGLVVQRDSSLTAETYGCLETPPGEALP
                     QRLLRLFSDLDRVMDRTTPDVVAVEKLFFGRNATTAEMVWQARGVVLLLGARRGIPLQ
                     EVTPNEVKMGVTGFGNATKGQVQGMVQRILSLDHLPRPDDAADALGIALAGIALYRYD
                     ALSARKDSSGDGSR"
     gene            5742..6326
                     /gene="ruvA"
                     /locus_tag="PL162_01540"
     CDS             5742..6326
                     /gene="ruvA"
                     /locus_tag="PL162_01540"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HQF90743.1"
                     /translation="MLRYLQGTVETVEGPLVVLDVQGWGFELQVSRAARSRALVGESL
                     RLFTALQVSESGVALFGFLRESERSLFQRLTSVKGVGGKVAMALLGTLEEETLVRSIL
                     SGDLATLSSAPGVGRKTAERLCFELRERLGSYAAGEPGIPANFPGALVLEALESLGFS
                     RSEAVAALRRTAEFSPPPATDEELLQAALKTLRK"
     gene            6372..7427
                     /gene="ruvB"
                     /locus_tag="PL162_01545"
     CDS             6372..7427
                     /gene="ruvB"
                     /locus_tag="PL162_01545"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HQF90744.1"
                     /translation="MSDDRLVHAPLGTNEEDLPLRPQALGDFIGQESLREKLTIYIAA
                     ARLRGEPLDHILFHGPPGLGKTTLAGIIAREMGGELRITTGPALERSGDLAAILSNLQ
                     SRDVLFIDEIHRLPAAVEETLYAGMEDFALDIVVGKGPMARNLRLNLPPFTLVGATTR
                     LGLLTSPLRARFGIVEQISLYDAEELAQIVLRGARSLGVSVEEDAAMEMGRRARGTPR
                     VALRLLRRVRDVAQVRQDPSISRALARGALEMLGVDEDGLDEGDRRFLRTVVELFGGG
                     PVGLTTLAAAMNEEAQTLEDIYEPFLIQRGLLERTPRGRRATAGAFEYLGHPVPADFG
                     PKQLTLELGRGDEGKEE"
     gene            7435..7659
                     /locus_tag="PL162_01550"
     CDS             7435..7659
                     /locus_tag="PL162_01550"
                     /inference="COORDINATES: protein motif:HMM:NF022596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 family protein"
                     /protein_id="HQF90745.1"
                     /translation="MGGLGKILVGLGAALLTLGLGLMLLERWGISLGRLPGDLIFRRK
                     NLVVFFPLGSMVLLSLGGTLLLYLWQRWGR"
     gene            7760..9148
                     /locus_tag="PL162_01555"
     CDS             7760..9148
                     /locus_tag="PL162_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HQF90746.1"
                     /translation="MKNSWQKGLILGLGMILVLFGEGVSSEAAEKPIAVGVGVGISQG
                     VLSSTKPFVLKDSKGKKVSVGKSATFTCSGSRLETPKGSLTLPVTAQGPAPIIWDKHP
                     YRGVLKLVPAAKGCNVVNVLGMEDYLRGVLKMETNPAWPPESLRAQAIVARTFAMKGL
                     GRHRSQGFDLCATTHCQVYRGINAEDPHTDKAVKATQGMVLAVGKELAATPYHSDSGG
                     ATANVGDVWGGSVPYLKAQAEPVAYTSPYSSWQLVLSPAQVEEALGRIDVRVGTVTGM
                     TVAQKDPFGRAVRLEICGTGGKAVVSSHKFRMAVGASVLRSTVFELTAGTTESSGAPM
                     PEETPSGEGEEASYFSESVEEGDLLIAMTRQGAFTTAELMDMLMHPETRAQHMAEALA
                     RGGRGAKTPPLPSKAPDRRAPGKGFVFRGRGWGHGVGMSQWGAKALADGGWKAEKILG
                     HYYPGTKLRSAY"
     gene            9105..10235
                     /gene="queA"
                     /locus_tag="PL162_01560"
     CDS             9105..10235
                     /gene="queA"
                     /locus_tag="PL162_01560"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HQF90747.1"
                     /translation="MGTTIQAPSFEALIKAQRGRGRGETLPATDGDAELFATASYDYD
                     LPEELIAQNPAEPRDASRLLVVPREGGELGHRRFSDLPTLLCPGDLLVLNDTRVWHAR
                     LLGAKPSGARVELLLLRPLDAEETLWEALVRPGRRLNPGAEVIIPGGRGVVGERRGEG
                     LREVRFELTSGTVREVLAAEGRVPLPPYIRSTEAPPGRYQTVYARQEGSAAAPTAGLH
                     FTEGLLERLEERGVEISFVTLHVGLGTFRPVESDDVRRHVMHEERCFLPEGTADAVAR
                     ARARGGRVVAVGTTVVRTLETFASDDGTLRPGTADTGLFITPGFRFRAVDALVTNFHL
                     PRSTLLMLVSAFAGYDRVMGAYRKAVEDRYRFFSFGDAMFIA"
     gene            10322..10933
                     /locus_tag="PL162_01565"
     CDS             10322..10933
                     /locus_tag="PL162_01565"
                     /inference="COORDINATES: protein motif:HMM:NF017747.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HQF90748.1"
                     /translation="MRWLKLHPEDYRKMPWKNGGGVTEEIALDPPGGSWEGPSRILWR
                     VSTAPVASSGPFSIFPGYDRLLAILDGGDLELRVEGKEPERLSPRGRVGSFPGDVPTE
                     GILLGSPCRDFNLMVDRARGEAMLRFVSLDEEGTLRSKKSVGILFAAQGEVGGRGPSL
                     EGEPFVLSPGDCLLWRREDGDPEAFFELRGSGWALWGEIIIRT"
     gene            11152..12114
                     /locus_tag="PL162_01570"
     CDS             11152..12114
                     /locus_tag="PL162_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HQF90749.1"
                     /translation="MKRYGVAVLVVLMVLGTVAGAGAKEFFSIATGGTTGTYYPLGGG
                     IAEIINSHVPDVQVTAETANASAANVNLIGSGQIEMAFAQNDIAYWGWKGENMFEGKP
                     YANIRGVAVLYPEHVHLISIQDAGIKAFGDIKGKRLSAGAPGSGVEGDVRAIFDVAGL
                     TYDSVKADFLDFANTTARFKDGQLDAGFVVGGYPVASIMDLATTAKIDLVNFDEAFLD
                     QLIAKYPFFAKGVIPANTYSGVDHDTVTPQVMAMLICREDLSEELVYNFTKAFWENVR
                     ELDKVHAKAKLITLETALQGLSVPLHPGAAKYYAEKGMAIPEIK"
     gene            complement(12350..12874)
                     /locus_tag="PL162_01575"
     CDS             complement(12350..12874)
                     /locus_tag="PL162_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90750.1"
                     /translation="MRVDPKNIPVDPTTWRPIVPKEPEDVTPETLRQAWEKTQGAVAH
                     ERDQILERHERIVQASQERQRKERQKKLLDLEKKDQAERRHDRFETLAQEQQLSREAQ
                     DRRGERRRAEEDAALQESTPHRPSLITPGREGESVEGDQRRRRAHQEARDAAAKERDL
                     PETPPRKGHRFWTF"
     gene            complement(13094..13732)
                     /locus_tag="PL162_01580"
     CDS             complement(13094..13732)
                     /locus_tag="PL162_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HQF90751.1"
                     /translation="MGQVQVIVEGITASGKTTVVKLLTERLGLQQIGEEFRTQYALLE
                     RYGRDQRWSFPMQLNFLTTRFAQYLMASESHDFILDRSIFGDMNYADTYHKLRYFSEG
                     QYQMYVQLFEFLSSYLVMPRVFIWTTCSFGTMMKRMGKAPGASEEPSGEEFLEELHSV
                     YSRFIANLPRNKRMRSYMVLDTDTRDLVHDNVARDQLVEEVRRFIAPEGENR"
     gene            14021..14248
                     /gene="rpmE"
                     /locus_tag="PL162_01585"
     CDS             14021..14248
                     /gene="rpmE"
                     /locus_tag="PL162_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HQF90752.1"
                     /translation="MKKDIHPKYGVCAVKCACGNAFETSSTHKGEMRVSVCSACHPFY
                     TGKKGKMVQAGRVEKFQKKYAAFSQQQAQQQ"
     gene            14334..15038
                     /gene="thyX"
                     /locus_tag="PL162_01590"
     CDS             14334..15038
                     /gene="thyX"
                     /locus_tag="PL162_01590"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HQF90753.1"
                     /translation="MGCQVSLLSCSPDPDRLVAAAARLCYSPASARDLAEVFLDEGRV
                     RPFLEKLRRSGHLSTFEHASFSFAVDGISRVCSHQLVRHRLASYSQQSQRYVSQEKAG
                     VVMPRSIREHPRAREVFEEAVAQTLLAYGRLGALGIPREDARYVLPHGGETRMMVTMN
                     ARELLHFFRLRLCRRAQWEIRDLAREMLRLVREVAPGLFAVAGPGCVETGACGEDRPC
                     GRPFVDTEDVLAHAVD"
     gene            15025..15978
                     /locus_tag="PL162_01595"
     CDS             15025..15978
                     /locus_tag="PL162_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HQF90754.1"
                     /translation="MRWIDLTTFFWGLFSLGVEKIPVGGQAVIEGVLMRGPRRWGLAV
                     RRPEGDIWHTSWGLAPWAARGVWKWPVCRGLAVMGEMLSTGMKALSRSAEIALGEEEK
                     LTGKDLALSALVALGGVVGLFLLLPMWGADLLTPWTGPSLVVRHVVEGVLRALVFIGY
                     LAVIGLWGDIRTVFAYHGAEHKTINAYENDAPLTPDSVEKFSRIHPRCGTSFLLVVVV
                     VSIVVFALAGNGPLWWRLSSRVVLLPLVIGLSYEFIRWSCRSPRVGSLVMGPALSLQY
                     LTTREPNRDQLAVGIASLQAALEEPPSEDSGREAPPGGEER"
     gene            15978..17054
                     /gene="prfA"
                     /locus_tag="PL162_01600"
     CDS             15978..17054
                     /gene="prfA"
                     /locus_tag="PL162_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HQF90755.1"
                     /translation="MEITKKLEEIVALYEDLERRMADSAAEASPQELQQLAKKHSDLQ
                     EVVHAFRDYQRRQKRLAESRQLASCGDEEMEELARSDVEELEEALIQDEDLLRRLLLP
                     RDPNDEKSVIMEIRGGAGGDEAALFAGDLFRMYTRFAERQGWRVEVMTLSETGIGGMK
                     EAIFRMDGLGAYSKLKFESGVHRVQRVPVTEASGRIHTSTATVAVLPEVDDVEVEVRN
                     EDLKIDTYRASGAGGQYVNMTDSAVRITHEPTGLVVTCQDERSQLKNRVKAMKLLRAR
                     LYDLELQKQQNQQAAERRGQVGTGDRSERIRTYNFPQNRVSDHRIGLTLHKLDLLLEG
                     DLDEMIQALTMADQAARLQQSALE"
     gene            17062..17973
                     /gene="prmC"
                     /locus_tag="PL162_01605"
     CDS             17062..17973
                     /gene="prmC"
                     /locus_tag="PL162_01605"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HQF90756.1"
                     /translation="MRVDEGRRWGAEILAAAGVVDPQRDVDLLLEEVLYRPWAHILAH
                     PEGLLSPSEEDRFRELLSRRASREPMAYILGEKGFWGRNFAVGPGCLIPRDDTEALME
                     TVLGVFPGGLFVDWGTGSGCLAATLALERPASRGVAVEASPRALVWARRNLRSLGVLG
                     RVDLVHGKAPEDLSLAPGVVDLVVSNPPYIPSLRLAGLMPEVGRFEPAEALDGGDDGM
                     DFYRMICPWASTVLKEGGYLAVEVGDEAQAAWLRDHGGASLRWQRFFYDLKGEVRGVV
                     WKKTSPVAYNRPGSSSLRLQGAGESRT"
     gene            17988..19172
                     /gene="trxB"
                     /locus_tag="PL162_01610"
     CDS             17988..19172
                     /gene="trxB"
                     /locus_tag="PL162_01610"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HQF90757.1"
                     /translation="MEKRQLVIIGAGPAGLTAAIYGKRAGLDVLLLEKGVPGGQINVT
                     DEIENWPGVEHASGPELAETFRKHAVKFGTEFRDSSVRKVELRDGAKVVVTEEGDIEA
                     EAVILATGASYKKMGCPGEAEFTGRGVSYCAVCDGAFFEGETIAVLGGGNSAVEEACY
                     LTQFAEKVYIVHRRDSFRADRVAVERALANPKIEPVWNSVVEAIEGGDMVEKLVIKNV
                     KTGEKSELPVAGVFVFVGSVPNDEPLKGLVAVNKAGWIETNDRMETSVEGIFAAGDVR
                     DKFLRQVITAASDGAVAAMAAYGYISEQLHLRSTLLDPERVVALFTSSVDEPQVRLTG
                     AVEEWCATSGKKIALVDGYKNARMVEHLGLAILPALVELNKGVVVKKAAVSSLKEVQD
                     FC"
     gene            19280..20215
                     /locus_tag="PL162_01615"
     CDS             19280..20215
                     /locus_tag="PL162_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HQF90758.1"
                     /translation="MIVTVTLNPAVDEGYLLPEFHPGGWFRSDRRVERTPGGRGINVS
                     MTLQQLGYENAAMGFLAGFNGEYVRDALRRAHITTNFVHVSGETRTNVYIVDEIGHIE
                     TGLAELGPYIPEEALARFFSNYERMLHRCSMVCLGGSLPPGVPQDIYRDLVNRAKDRN
                     IATFVDAEGPALMAVLEAVPTVVKVDHRYMSRVAGIATTSLDKIIEIVSRVHEKGVDY
                     ALTSYRTYGYVFHTPQGVFLAEMDRKGVVSLFGSSEALMAGLVVARAERMPVDETLRF
                     AMACAQEDSFHVEKGVRGRREVEAIMDQVHLEKLA"
     gene            20239..20697
                     /locus_tag="PL162_01620"
     CDS             20239..20697
                     /locus_tag="PL162_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQF90759.1"
                     /translation="MQIGDVIDRDLTAVTGDMAVGVVVEILTRHRLSGVPVVDDEGRV
                     LGFVSEEDIVRAALPGYFEYLSNASTIPDIGQFQARLRRIGREPVSKYMNTAVTVFKE
                     DDSDFSVAMAMISKNARRSPVVRDGVLVGAVNRGDLLKRILTDGEKEEDR"
     gene            20758..21261
                     /gene="fliS"
                     /locus_tag="PL162_01625"
     CDS             20758..21261
                     /gene="fliS"
                     /locus_tag="PL162_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HQF90760.1"
                     /translation="MDDKQVRHAQETYRINQIKTASREQLLLITYDIGIQACKMGEEA
                     MRRGNVEETNEALQRAQSVVRELMVTLNVEVGGDVAKQLMNLYDFLYYELVHANVQKD
                     MDRLVTVRTMLQELRQTWQEVADKLHAEEEEPMALPQEAVPGPKAVNMVAAGGRPLLH
                     GGLNVAG"
     gene            21251..21721
                     /gene="flgN"
                     /locus_tag="PL162_01630"
     CDS             21251..21721
                     /gene="flgN"
                     /locus_tag="PL162_01630"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HQF90761.1"
                     /translation="MLVDLKERLKGLLTRELALYDRLESKVAEEELSIQTEDWEGLLR
                     ALQEKQALISEQETLQESWRSCAVLLEVDGGRESAEFWDTLARKMGHNSYQEISVLVE
                     NLREVARRTLERERAVQATLEAHLEALRARMRQFQKGKEAFKTYARTGGASSAP"
     gene            21749..22846
                     /locus_tag="PL162_01635"
     CDS             21749..22846
                     /locus_tag="PL162_01635"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HQF90762.1"
                     /translation="MRKWGGRGRRVMAALLLCLLWGSWAEALTSDDEKEIAFGCKIAE
                     TVDASGQRVGNPAVLARLAMVGKRLTPFLTRDLPFEIRCISLSGDRRPNAFALPGGIV
                     YVTESMLDFVRSDAELAGVLAHELVHTDRKHGMIQSARNSRLSLLSLLVAVASRGEVA
                     AMVLANLVQVAIMGNYSQDLEEEADRQGLDLLRKGEYDPAAMVTLLERLEEERLKHPY
                     LDPGVYQDHPDTMDRVNYIIETMRDLRWPLHRKGPLNLLRVRGEQEGTWWILRVDGDE
                     VWRCPAQEWSSADRRSLTDALDGALQLELPPADLEVVSHPQGSFLRLGQVRLLGEPLP
                     PGSSSLKDFRQAIVRVLDGARRKHPLGQYLK"
     gene            23091..24956
                     /gene="dnaX"
                     /locus_tag="PL162_01640"
     CDS             23091..24956
                     /gene="dnaX"
                     /locus_tag="PL162_01640"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HQF90763.1"
                     /translation="MGYLTLYRKYRPQRFSDVVGQSAAVDVLRHAVEERRMGHAYLFS
                     GPRGCGKTSVARIVAKALNCLDLQEGGEPCGACSQCHAIVSGESMDVLEIDAASNRGI
                     DEIRELKSHVGLAPFSGKFKIYIVDEVHMLTEPAFNALLKTLEEPPPHVVFLLATTEP
                     HKVPVTIRSRCQHIPVRRLEGHTLVALLREVLRAEGASGDEDALWEIARQADGGARDA
                     LSLLEQALALGRGHLSREGVDDLLGGASRSELEQALVVLRKDPAGSMAKFHAMLSRGA
                     SLERLLDGYFLLLKDLWVVSSWSAKALEGGGLSDRERAFVEAEAPHWDRRRLWRGMQF
                     CASWLPRLRSGVRGEVVLGLLVGTMESGGAPEGEERPTPRPPLPSEKEESNERPSAAS
                     DKGLPPPGRDEGREGRKPLRLPQEEAPGGGMPPPSEGTPEVAAQEKREAFPEAPSTPD
                     PSPEGWKRMAAALERQDPGIGAALLGLRALRDEDRLILDVPEDFPLVFHALGGGKGSL
                     LLVRAAHLFLGLRSVELRWRDHRRVFPGGTEGEREQEEKKEGETPSWQSFIPDRVREI
                     LPSGGPENLPTPKASEPSALEQILSCAPAEVLMVRRELGSEEEDAEIAGHREDEE"
     gene            24953..28363
                     /gene="dnaE"
                     /locus_tag="PL162_01645"
     CDS             24953..28363
                     /gene="dnaE"
                     /locus_tag="PL162_01645"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HQF90764.1"
                     /translation="MTRPFVHLHVHTEYSLLDGAIRCGDLARKAAEYGMPAVAMTDHG
                     VLYGAVEFYEKCCQAGVKPILGCEVYVDPEGHTRRDKGSRNNHLVLLAEDDAGYANLV
                     KLVSIANTDGFYYKPRVDHDLLARYARGLIASSACLGGEIPALILEGNEEGARQRACF
                     YNDLFGKGNFYLEIQHNAIPEQARVNKALIGLSRETGIPLVATNDAHYLNAEDASWHE
                     ILLCVQTKSTMSDPKRYRFGAADFYFRSPEEMDRLFGAEVSEALDATVAIAERCNVKF
                     CFGEYRLPHFAVPPGETLESHLERLSRAGLRDRMGGAPSPEYIERLEYELGVINRMGF
                     AGYFLIVADFINAAKTRGIPVGPGRGSAAGSLVAFALRITELDPIHHKLLFERFLNPE
                     RISMPDIDTDLSDKRRGEVLDYVVQKYGRDNVSQIITFGRMMSKGAVRDVARSLDMPY
                     AEADHLAKLVPDGVKSLQEAQEKSPDLQALVQSDPLARKVLDIASHIEGLARHSSQHA
                     AGVVIAPCPVSDVVPVCRIGENQVVTQFAMEPVEKLGLVKMDFLGLRTLSLVEDTLAN
                     IKSNGKIPPDLEKLPLDDEKTYALLQQADTLGVFQLESGGMRQLLRRMAPDAFEDLVA
                     VLALYRPGPLESGMVDQYVECKHRRREVEYPHPKLQEVLRETYGVILYQEQVMQIAAV
                     LAGFSLGEADLLRRAMGKKKEEVMREQRGKFVAGCLKNGVGERVAEEIFDVIQKFAGY
                     GFNKSHSAAYAMISYQTAYLKAHYHAEFLAAVLSSQIGSRIDVLARYVRSVRDAGVAV
                     LPPHVNESGADFTVVGDAIRFGLSAISKAGDAAVEAILSSRRKKGPFSSLWDFLKRVD
                     TRVVNRGVVENLIRSGGFDGLGGNSRQLLEALPSLSDLLARRSSDGGQGSLFGASGSE
                     EEERPPLPDVPEPTPRERLEMEKETLGLYLSGHPVDDYVHRLLRFTNCVIADLSFWKA
                     PDSLVSVGGMITERREALTKKGDPMGVLILEDPDAKVEVVCFPRNWAEMRGIFSVGDV
                     VVVTGRVQERGDTVLLAEKIIPLEVVERDQVPQVRLRVDGRGISSEVVREVLRELKNF
                     PGKSPVLLEVQDGSTMALLRLRDLRVQKGVLSPEKLKDLSQGRVELVC"
     gene            28409..28681
                     /gene="mtrB"
                     /locus_tag="PL162_01650"
     CDS             28409..28681
                     /gene="mtrB"
                     /locus_tag="PL162_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp RNA-binding attenuation protein MtrB"
                     /protein_id="HQF90765.1"
                     /translation="MPGSQGREALGAGGESILGDFIVVQALEDGVTVIGLTRGHETRF
                     THTEKLDRGEVWISQFTEHTSAMKIRGKARVLTRHGVAESGRGDHA"
     gene            28674..30425
                     /gene="pyk"
                     /locus_tag="PL162_01655"
     CDS             28674..30425
                     /gene="pyk"
                     /locus_tag="PL162_01655"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HQF90766.1"
                     /translation="MRRGKIVCTLGPACSDYDTLRAMASAGMDVARFNFSHGSYETHA
                     LNLEQVRRVEQEMGRPLATLLDTKGPEIRTGTLKERAPVTLVEGKTLVLTTREEEGDA
                     ARVSVSYDRLSQELSPGMDVFIDDGTLHLRVEHVEGEEITCLVLVGGELGERKGINVP
                     DAILSVPALTPKDVEDIRWGVEHQMEYIAVSFVRTREDVMDVRRVLEDFGGTLGIIAK
                     IETKLAVQNLKEISQVVDGMMVARGDLGVEIPTEDVPLVQKRIIDLCRSQGKPVIVAT
                     QMLDSMIRNPRPTRAEANDVANAILDGADAVMLSGETAKGKYPVLAVETMGRIVTRVE
                     SERDRWQRGCTVPIAATTVPDAVSHAAVRIAEEMKARVIISLTESGSTASMVSKFRPV
                     CPVLAGTPIKRTWRKLALVWGLYPILQDHAATAEEALEGALAASLERHLVSEGDLVVV
                     TAGVPVGIPGTTNMVQVHTVGRILLKGLSLIRKEASGRVCRAATASEAREKMRPGDVL
                     VAPQTDQSFLPAMQGASALVTEEGGLTSHAAIVALELGIPCVVSAEDALRILEDGMLV
                     TVDGIRGVVFSGKVRLR"
     gene            30472..31314
                     /locus_tag="PL162_01660"
     CDS             30472..31314
                     /locus_tag="PL162_01660"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HQF90767.1"
                     /translation="MGLLGVHVSVAGGLGRAFDRGEALGCEAIQIFTRNQLQWHGADV
                     SLGESEAFFRRWRQSSVKEVVSHASYLINLVGDPELRSKSLDALVREIRRCDALGIDL
                     LVLHPGSFRGESEAEGLTLLGDALCRTLDATEDSRVRLLLETMAGAGNTLGGKLEHFS
                     AALDMVEDHPRIGVCLDTCHLWAAGYDFRTPATYEHLMGAVNKAVGEDRVGCWHLNDS
                     KTPRGSRRDRHAHVGEGEMGAGPFGLVLQDDRWDEVPCILETPKDGPGDGGNLAVLRK
                     MAGK"
     gene            31530..32363
                     /gene="cdaA"
                     /locus_tag="PL162_01665"
     CDS             31530..32363
                     /gene="cdaA"
                     /locus_tag="PL162_01665"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HQF90768.1"
                     /translation="MAVDIRWQDVLDIAIIALILYRLLLLLVDTRAMQLVKGMMVLAV
                     AALLAKVAHLEALSWVVGKLMGVLLFTIPVIFQPELRRLLEELGRGNIWRRRLVQERA
                     ESFADEVLRALIHLQSRRIGALLVLQRETGLRDVWRSGVKLDAEITQEVIVSIFWPGT
                     PLHDGAAVFDREKILAAACYLPLSDTVNLSRACGTRHRAALGVSEVSDALAVVVSEER
                     GEISLATNGHLARDLKEAQLRKMLLVYFSNEQETMPFFEKVKRLFSSPKPQGEAHEKK
                     Q"
     gene            32347..33561
                     /locus_tag="PL162_01670"
     CDS             32347..33561
                     /locus_tag="PL162_01670"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HQF90769.1"
                     /translation="MRKNSRYENILDSRFFLQIISVGLAMLLWFYVAGDRTSETVKSF
                     RLPLEYQGLGPGLSLNNPSSFVDVQVTGKVAPLRGLTQENLSCFVAIRDLGPGKYTLP
                     VTPVLPSGISLVTVSPSSVRVEISRNVEKTMPITLRVQGDFPEGYALVAASLDPPEVL
                     LKGPDTSLGRISEVWAAPRAQDLVDDGDGVMSLPLSAQFSGLRDDRVWMEPKDVRLRF
                     ELSRELPQKQVPLRAHVFGDVDPNYTLAMVTVRPPTATVWGPSSVLAGVSFLDIESVD
                     VSGLRGNATFSANILSPGEGLTVYPFPTVQVQATVRPVLISRTFSSVPVDVEGRSIYP
                     RWKVDPNKVKVTLRGLVGVLSGDGAPRAYVEVTNLVSRRVTVPVRLRGRSADVEVVEI
                     SPPMVTVYALVE"
     gene            33598..34968
                     /gene="glmM"
                     /locus_tag="PL162_01675"
     CDS             33598..34968
                     /gene="glmM"
                     /locus_tag="PL162_01675"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HQF90770.1"
                     /translation="MLTGSGRVRCLFGTDGVRDVANRGMLTPEMALHLARAFVWWLVR
                     RGVSRPRVVVGRDTRRSGDMLEAATMAGLMSAGADALTVGVLPTPGVSFAIGSVGASG
                     GVVISASHNPAEYNGIKFFDSQGCKLSDEDEASIEEYMDDHLLDDWRPTGASTGVVGD
                     GAFLRERYQHWLESLAGEHTWPGRLVVDCAHGAAVPVAGPLFALLGADTVVTGDRPDG
                     LNINEDVGVMHMSHLVGCVKEHRGQLGVAFDGDADRVLFVDRQGRTLDGDVLLWVLAR
                     WLRRRGTLGSGVVATVMSNMALEEHLGEEHIPVFRCPVGDRYVLETMKREESQLGGEQ
                     SGHIIIREFVSTGDGLCSALLFLRACRELGEDVDTLVDRFARYPQILRNLHVKDKRRI
                     LEDDNWQVFLNQAEARMQGVGRILVRPSGTEPLVRILVEAKDPTLVQAVSDDLEARIC
                     ALNAEN"
     gene            34990..35862
                     /gene="galU"
                     /locus_tag="PL162_01680"
     CDS             34990..35862
                     /gene="galU"
                     /locus_tag="PL162_01680"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HQF90771.1"
                     /translation="MAFSRKVTKCLFPVAGLGTRFLPATKETPKEMLPLIDRPIIHYG
                     VEEAVSSGCGDVIFVTGRGKRSIEDYFDRSWELEDLLERRGKDDLCRLVRDITQMARF
                     SYVRQSEPLGLGHAVLCGELMCQDGPFGVILPDDVMRGDPPVMKQLIDVHECHGGSVI
                     ALEEVSPEDTSRYGIVAADDVGNGVFRLKDMVEKPVAGRAPSRYAIMGRYVLSPTIFE
                     ELRSLNPGAGGEYQLTDALRSLLLREPIWGYLYRGKRLDCGTKQGWLRATITMAWEDP
                     ELKGILQEVLGDEA"
     gene            36193..38028
                     /gene="glmS"
                     /locus_tag="PL162_01685"
     CDS             36193..38028
                     /gene="glmS"
                     /locus_tag="PL162_01685"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HQF90772.1"
                     /translation="MCGIVGYVGPRNACDVLLEGLRRLEYRGYDSAGLAVWTAEGLRT
                     IKEVGKVAELADAVRAAGFDGFRGIGHTRWATHGGVTQANAHPHADDRHRFALVHNGI
                     VENYSELREELEKQGAIFSSETDSEVVVQLLARIMGEIASMPEALRELQGRLQGSFAL
                     AIMDGEDPDHFFCVRRGSPLVLGLGKGEAFCASDVSALLPYTREVIYLEDGDMARLGP
                     CGATMWNATGEEIHRPGSMVDWDVTMAEKDGFPHYMLKEIHEQGGVLRAAFKGRIFPG
                     GVDLSGELDWDDALIRSWKRIHVVACGTAHYASLVGQRVLERWTGLEVLADIASEYRY
                     RPLRADADTLAIFVSQSGETADTLAALRKARSEGARCLAVTNVRGSTLAREVHDTLLL
                     RAGPEIGVAATKTFMGQMGTLYALALYLGLKRGGVSSAEAKRLSEGLLQLPYRVEALL
                     TRLDASVRALAARYAGARSFLFLGRGHSYPVALEGALKLKEISYVHAEAYAAGEMKHG
                     PIALLEPELPVFVVAPRDSLYEKTISNIQETRARKAPVLAVGTEGDTLLSSHVEQVIA
                     VPWAEEELTPFLTVIPLQLFAYHVALSRGCPIDQPRNLAKSVTVE"
     gene            complement(38245..39078)
                     /locus_tag="PL162_01690"
     CDS             complement(38245..39078)
                     /locus_tag="PL162_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQF90773.1"
                     /translation="MISPDATVGLAIKDPKVRMALEGLLRSLGLACTTWDDLGDVVMG
                     VDHLDAPLEVLFLGWGQTQEGEASQIQAIRLHARGSSTQFFALSPEWSDIRARMRARV
                     AGCDDLLALPPDPNQIRRALGFPVEEVSAPSPAPAPVPEKTTVSEDTPFPPDFRVLVV
                     DDAAVVRVGLSRLLSKMGCVVVEAENGQDALRKLPSANPHVIITDLLMPYMDGFGFMQ
                     NLREKTQWEHVPVIVVSGYGDKPRLIQALRHGAVDFIVKPFKPEVIRQKLLNMLHQKK
                     D"
     gene            complement(39236..39751)
                     /gene="mrtS"
                     /locus_tag="PL162_01695"
     CDS             complement(39236..39751)
                     /gene="mrtS"
                     /locus_tag="PL162_01695"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_274372435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SYNERG-CTERM system CAAX-type protease"
                     /protein_id="HQF90774.1"
                     /translation="MARFALAAFMLYGPFLWCVLRRESPQRYGLTWRWDPALVRSVLF
                     WCGFTLIPLTGVAWFWPTGGIPRTIPWGGVGQLVMAGTTAAIVEEIFFRGWVQSSLRP
                     WVGTMGRVVLVSALFAASHMIFQSRPLFLATFFPGLIMGYLRERHDTVSAPLLYHALC
                     NVWAVWFFPNP"
     gene            complement(40179..40619)
                     /locus_tag="PL162_01700"
     CDS             complement(40179..40619)
                     /locus_tag="PL162_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HQF90775.1"
                     /translation="MWTLEEGLQTLKAQGAKITAQRIAILKRLEGRKDHPSAEMLYKE
                     LRATFPTISFATIYGTAELLARAGLISILTIDDRHVIFDPNAAFHGHFKCSQCQGVED
                     IPLPEKGLPSFGGDRPHRVDEVQVFFYGVCPSCLASAQERAGNA"
     gene            40956..41030
                     /locus_tag="PL162_01705"
     tRNA            40956..41030
                     /locus_tag="PL162_01705"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40988..40990,aa:Gln,seq:ttg)
     gene            41157..42536
                     /gene="glmU"
                     /locus_tag="PL162_01710"
     CDS             41157..42536
                     /gene="glmU"
                     /locus_tag="PL162_01710"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HQF90776.1"
                     /translation="MGNAVRRGALVLAAGKGTRMKSATPKVMHSILEEPMISYPLRAT
                     LGAGYDRVAVVVGFGGEEVQAYLQAHWPQVEVLWQREQLGTGHAVMEVRGWWQSLDEL
                     VILYGDMPCLTPEALRALDKSTAEGAFLSFRAGDPRGYGRVVRGAGAVRIVEDRDATP
                     EEQRLDEVNSGIYRFSVPLMAKALETLSPANQQGEYYLTDVVHALGATGRGVDPVLWP
                     EAEDLEGVNDPRQLAAATAVLRDRILGEAMGNGVRCLDPSSVWVGPEVVLEEDVFLAP
                     QVQIFGKSRIGAGSTVGSGSILRNAEVGRSCRLVSYVCLQDSVVRDGVSLGPFVVIRD
                     GSCFEAGSLAGKFVEVKKSIVGRGSKVPHLSYLGDATVGEGTNIGAGTITCNYDGVRK
                     NPTIIGDRCFVGSDTMLVAPVRLDDDVTTGAGSVITQDVPPGALAVGRARQRNVEGWK
                     ARRRAKEEG"
     gene            42536..43507
                     /locus_tag="PL162_01715"
     CDS             42536..43507
                     /locus_tag="PL162_01715"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HQF90777.1"
                     /translation="MVTRLREIKIFSGSANPAFAQEIADHLRVSVASSKRFRFADGEI
                     GISIEESVRGADVYVVQSTCRPVNENLMELLIMVDALRRASAYRINVVTPYFGYARQD
                     RKTRGREPITAKLVANLLTHAGVHRVIAADLHAGQIQGFFDIPVDHLTGMHLLAAHFR
                     EQLKGSIDQGEVVVVSPDVGGVVRARKFAGQLNADLAIVDKRRSYEKTNSCQVMEIIG
                     DVQGKDVILVDDIIDTAGTMVNAAAALVERGARSVYACATHGVLSEPALERLRASCIQ
                     KMVLANTIPLPPDEKPDKIVQLSIAPLFAEAIDRIHSDHSVSILFGA"
     gene            43568..44227
                     /locus_tag="PL162_01720"
     CDS             43568..44227
                     /locus_tag="PL162_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HQF90778.1"
                     /translation="MSGFIPITLESREGIGKEKSGQLRAAGYLPAVFYGPDYKDGMPV
                     RFQAKSLLPHLRKSHWETLRLDVTLPDGKQEMALLRDLQRDSISDEVLHLDLYQLVRD
                     RKVRVAVPVRVINREGCPGIKAGGVFELLHRVVEMEVLPREIPDDIVIDAASLDKGKG
                     FRVRECALPESAEVLLGPEDFVALISEPRAEEEEEAEATAGSMDVEVVQKGKKDKDGA
                     A"
     gene            44279..44860
                     /gene="pth"
                     /locus_tag="PL162_01725"
     CDS             44279..44860
                     /gene="pth"
                     /locus_tag="PL162_01725"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HQF90779.1"
                     /translation="MRLIVGLGNPGPEYAWSRHNAGWMVADAVAEKFGMGTPAVKFSS
                     LFWQGRLPDGEKVLCAKPVTFMNLSGVAVREMVAYHRLAPERILVLYDDVALPFGQLR
                     LRGKGSSGGHNGMASVLGALGTLEVPRLRVGVGPLPAGWDLVSFVLGAFPRDQREAMP
                     GVVDRGVRAVELWIAEGLEGAMGRVNGAGKIQG"
     gene            44976..>47126
                     /locus_tag="PL162_01730"
     CDS             44976..>47126
                     /locus_tag="PL162_01730"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HQF90780.1"
                     /translation="MTAWRDGGGQFWDLPSEAWYGGAGVHLATAGGARPWACAHPKGP
                     MVVVLADLRQMETFAEDWKALDPEKSGGTLSVLRELPLGPEAAGNRAYWMQRGETLRR
                     WRHRGGVLVATPGGLLGPYEEGTETLSLREGEVVGWNRLLAWLTEGGYGRSDLVWAPG
                     QWVSRGGIVDVFDPAEPLPLRIEFCDDEISSIRYFSPENQRSVGSLGTVELRRLSDSR
                     SLRRGIAAERIPEDVPVVFFEPRDVELQGDLYEELWNGLREQEDLPEIPPWQEVALSL
                     FRHPRLRVSRALAPGDHRMAIQELPPFRGKIKDAEGFVAAWRDRGFSLLLVSRTEAVR
                     LWGEQRGFHVRSGSLSGGFLDLDSRCGVFSDAELVGVSLSSPSSGWYGVPREWADRLA
                     PQSWVVHEDYGVSRFLGTQGVETPQGEQDYLVLEFAQGKRLLVPVAQFGKVTPFSALP
                     EEEPTPDALSGSRWRKAVQRARERAAEAAKKLANLYALRETTAGMAFPADGEMLRMVE
                     HSFPYRETEDQLRAIRDVKKDMEHHYPMDRLVVGDVGFGKTEVALRAVLKAVEGGRQA
                     AFLVPTTILAQQHHGTFEGRLAGLPLRVESISRFVSPARQRRILADTAEGKVDLLIGT
                     HRLLQKDVSFARLGLLVVDEEHRFGVLSKEEIKGRFPGVDVLTLTLRPFLGPFSWPWE
                     GCAPYPSSPLPPSTVSPSFRWSLPGETSWCARPSS"
     assembly_gap    47127..47226
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <47227..48267
                     /locus_tag="PL162_01735"
     CDS             <47227..48267
                     /locus_tag="PL162_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HQF90781.1"
                     /translation="VLSVVSPWRDELVRKALQRELARGGQAFFVHNRIPQLESRERWL
                     REVLPGSRVAVAHGSLPSEGLERVMEDFFLGKSDILLCTTIVESGLDVPQANTLIVDD
                     AQHLGLAQMYQLRGRIGRRSAQAFAYFLYPEGETLSREAQERLEAIGEMGEFGSGYRL
                     AMRDLEIRGGGDFLGLAQHGHGPRVGFSLYCSLLEKELTAVMGGKISKTAIAINFPLG
                     IPRGYVGEESVRIALYRRILRLDSWEEVQSIERELRDRFGPIPESVGSLLDGARIRAL
                     GSHFGVEYVDGRADRTEVGGETLGRSPLGRALPGWMPGKRGLVGPGGARGLRDLAEAM
                     KILSEASEGTKS"
     gene            48282..49208
                     /gene="mazG"
                     /locus_tag="PL162_01740"
     CDS             48282..49208
                     /gene="mazG"
                     /locus_tag="PL162_01740"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HQF90782.1"
                     /translation="MTGEEESAPRGAVLESLRKNDEEGAPEEGPRDSGSSFSRLMDVM
                     ARLRAPGGCPWDREQTWESLKPYVVEEAYELLDAIDSSDDEGIREESGDLLLQTVFIA
                     QIAREEGRFDMGDVLEGLVEKLVRRHPHVFSDVSAETSDAVLRHWERIKSVERRCRGK
                     DLSMLGGIPRSLPALLRAYQMQHRAAKVGFDWPQGDPQPVLNKVQEEIEELREALIRG
                     DKDQVAEEMGDLLFAMVNFGRHLGIDAESALQAACHKFARRFGSVEEAVRLQDRPWES
                     FSLEELEEFWGNAKASERGPEASDPPRFGHEA"
     gene            49391..>50710
                     /locus_tag="PL162_01745"
     CDS             49391..>50710
                     /locus_tag="PL162_01745"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HQF90783.1"
                     /translation="METALRDAHQSIMATRLRTEDMLPVAEVMDEVGYASLEVWGGAT
                     FDTCMRFLDEDPWERLRLLRKRFKKTKLQMLLRGQNLVGYRHYGDDVVREFVKRAVGN
                     GIDIIRVFDALNDLRNMEVAADQVKKEGARLQMALSYTISPAHTLEAFADLSVQMKDM
                     GADSLCVKDMAGLLSPVAARSLVQSMKDRTGLPVQVHSHYTSGMAAMSYLAAIEAGAD
                     VVDCAISPFAMGTSQPATETLVAALAGGRFDTGIYLEKLEPVSRYFKEMAEKYHKMMM
                     GLEGVDINILLYQIPGGMYSNLVSQLKEQNALEKLEAVLQEVPRVRREMGYPPLVTPT
                     SQIVGSQAAANVLVGERWKVVPKEVRMYFLGYYGKSPAPVDPEVARKATGGEEPITCR
                     PGEKLGDELPGARAAAGPYLLQPEDLLSYVLFPSVAKDFLVKKFARET"
     assembly_gap    50711..50810
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            50820..51011
                     /locus_tag="PL162_01750"
     CDS             50820..51011
                     /locus_tag="PL162_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HQF90784.1"
                     /translation="MRPALQSHGGDIEFLGADLEEGSVKVRLTGACGGCPFAQETLRT
                     QVEEVLKGQIPEIRRVVRG"
     gene            51124..52065
                     /locus_tag="PL162_01755"
     CDS             51124..52065
                     /locus_tag="PL162_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HQF90785.1"
                     /translation="MPLRGEELVALLGKNDEHLPLLEERYPVRLFVRDGELRVEGPDA
                     SAVETVGTLLSELAKVVRQGHTLSAAEVRYAMDALAQGELPDMASLHGEVICFTSRGK
                     PVRPRTLGQRRYIDAVRRNSVVFCIGPAGTGKTYLAVALAVAALKAQSVSRVILVRPA
                     VEAGESLGYLPGDLKEKIEPYLRPLYDAFYDLLSPERFLRYAAKNVIEIAPLAYMRGR
                     TLNDSFIILDEAQNTTPEQMKMFLTRMGFGSKVVVTGDITQVDLPAGKMSGLRVISSV
                     LQGIEGIEFTSLDNRDVVRHEIVQRIVLAYEDFESRS"
     gene            52040..54154
                     /locus_tag="PL162_01760"
     CDS             52040..54154
                     /locus_tag="PL162_01760"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HQF90786.1"
                     /translation="MKISRVGRSVDRWWFFALSRWKETLPLWCMRGGLVLVALAIFLG
                     GWYLDRDLEGFSVGQPAPRTFLAVFRMRFEDVETTEKLKLRIADRVVGVVVQDPSRVE
                     DILVKLDQLQENRAALLPVLSSPLVEIVERLPEGERILVLREARRLGEMLMEKGMLGV
                     GRRAPELWEVLDDTSLSWSQKNVAFQLLDQILLHAQYDDRRLTAAVERQLQDSVQPVV
                     RTLDVGQVIVEQGDVVTPFLGSLLRSQGFPEALFPWGSLLLVMAASFLWGLWLTSFPI
                     REKALEDRRSLVFVTLLLAVSWGVEVGTTYFRMAGMGTLVLAGWSFLALPPSFAFQLV
                     LGGGALGVLISESGGLAHISLGMMVVSVVSGTGFLLFRQVRSRVHLWREIIVLGLVTS
                     GVALLAWWGSGAQITATLVLRFLSASAILGALVVAVLPLWENVFDILSPLRLLELSHH
                     ASPLLKRLQIEAPGTYHHVLMVGTLAERAADRLGMNSLLVQAGAYYHDIGKLKRPQFF
                     VENQMSGVNVHDDLSPSLSALVIIAHVRDGMEIATEARLPKRLRQFIQEHHGTTHLSY
                     FFRKALAQGERVDREQFCYPGPCPQSRETAVVMLADSVEAAVRAAWGAPSRETPGGDS
                     PRTPEGKDPSRQDAIQRPAAIQSIVQGVVETKMREGQLSHVDLSLKDIALVEKTFVET
                     LQFMYHSRQTKDDVGPKAPEGR"
     gene            54160..54678
                     /gene="ybeY"
                     /locus_tag="PL162_01765"
     CDS             54160..54678
                     /gene="ybeY"
                     /locus_tag="PL162_01765"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HQF90787.1"
                     /translation="MPMAVDFYGRELLGDSWSLPQEAERALEDVFFQILRAHCGPLAR
                     SKTSVEVSINLVSPEEMAELNEAYRGTAGPTDVLSFPLWEEGERFSPPVGWKDLPLGD
                     IVVCDEVVAHHAGERGVPVTEELALVLVHGFFHLLGWDHDTPDKEIAMWREQDGPVQS
                     LLRQWGLGAPKD"
     gene            54697..55980
                     /locus_tag="PL162_01770"
     CDS             54697..55980
                     /locus_tag="PL162_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HQF90788.1"
                     /translation="MSSDIGSSVALLVFLLIISALCSATETAITAVGRARLLALQERQ
                     PQRRGLYGWLMRETQGALTMTLVANNAVNIGASAVATSLTISLFGPSAVWLSTLLMTF
                     VIVVFGEIFPKSAAMVNAERFLVWGLPAVRLFHAILVPLLWFFSGIVRFLGRILRVDL
                     SSQRTFVTREEIEQVVSMGEVSGALEEDERRMIHGVIAFEETRAYEIMVPRTDMVAVS
                     RDASVSEAVEVVRREGHSRLPIFEGSLDRIVGILYAKDLLHALAEGATDAGIFEFRRD
                     PLFVPEVMRIAELFDIMKGGRVHMAIVVDEYGGTAGLVTLEDLLEEIVGEIQDEYDEE
                     TPPIKQEEEDTFLVEGQVNLEDLGDVLGDSFHAEDVDTVAGLALSLAGEFPEEGQTFR
                     YGAWDLEVLGVEEHRVKLLRLRHRLEEQKSEEEGR"
     gene            55977..56414
                     /locus_tag="PL162_01775"
     CDS             55977..56414
                     /locus_tag="PL162_01775"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HQF90789.1"
                     /translation="MTGFLPPGGPWGDGFPEALMAQARQARERAYAPYSRFPVGAALL
                     MEDGAVLWGCNVENASYGLTCCAERNAVGHMVLRGSTHPRAVAVAGPPGEPCFPCGAC
                     RQVLSEFNPALLVVVERGESLEVVSLEELLPRSFGPRDLESSR"
     gene            56411..>56869
                     /gene="era"
                     /locus_tag="PL162_01780"
     CDS             56411..>56869
                     /gene="era"
                     /locus_tag="PL162_01780"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HQF90790.1"
                     /translation="MTCPGDEERKEGGPEPSGPLCPSAVLTFRSGFVALLGRPNVGKS
                     SLVNRMLDDKLCIVSPVPQTTRHAIRCIRTTDRSQMVFVDTPGLHEPRTALGTYMVDR
                     VRKTLDEVDCLCLVLDARRPQGGDEEKMLHAVLAGATTPLVVAVNKIDGIS"
     assembly_gap    56870..56969
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <56970..57266
                     /locus_tag="PL162_01785"
     CDS             <56970..57266
                     /locus_tag="PL162_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HQF90791.1"
                     /translation="RDEIPHSVTVAVEEFKSPDEYHERETGYIRATVYVEREGQKGIL
                     IGEGGRFLKRIGMAARGELEARLGYPVYLELWIKVRAGWRRSPRDLKHFGYDET"
     gene            57412..58020
                     /locus_tag="PL162_01790"
     CDS             57412..58020
                     /locus_tag="PL162_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO C-terminal
                     domain-containing protein"
                     /protein_id="HQF90792.1"
                     /translation="MWVSVPGGAKGSVRFGGATEPFVWGVFHLYRSPRRLHLRDVDVK
                     EHFWELRSRPAWLRRGLSWDALLIRGLLPGHPCDEVLPPFFWALSFLREGVSEEVVEW
                     RFFWKWLHAWGRAPDLERCASCGASLEAEGWDPEEEGLRCAACVPRQKEGAAKGFPLL
                     KDLHRAAWLPPKEFASWTPENFWPPRWARANGWLRSILVRSG"
     gene            58107..58985
                     /locus_tag="PL162_01795"
     CDS             58107..58985
                     /locus_tag="PL162_01795"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HQF90793.1"
                     /translation="MNLQDIVMRLERFWADQGCVIQQPYDMEMGAGTMSPATTLRVLG
                     PEPWRVAYVQPSRRPADGRYGENPNRLQHYYQYQVIMQPAPEDIQEIYLDSLRALGID
                     PRDHDVRFVEDDWESPTIGAWGLGWEVWLDGMEVTQFTYFQQVGGIDMHVVPAELTYG
                     IERIAMFVQKVDNVYDLSWVGKVRYGDIHHKGEVEHSTYNFDLSDTAMLFQLFSMYEA
                     EARRLAEAGFVLPAYDYVLKCSHTFNLLDARNAISVTERTGYIGRVRSLASLCCRAYA
                     RQREEMGYPLMGKFGA"
     gene            58998..>60042
                     /gene="glyS"
                     /locus_tag="PL162_01800"
     CDS             58998..>60042
                     /gene="glyS"
                     /locus_tag="PL162_01800"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HQF90794.1"
                     /translation="MNERDLLLEIGTEEIPSRFVAWAREELASLARQEFASAHLKFRD
                     VGVYGTPRRLALFVRGVAINQEDLVQEHKGPAWAQSFDVYGHPTNAGRGFAKSRGVPV
                     ESLEKRVVEGVPYAFVLTRIEGRPTAALLPELLDRLLRRIVFPKNMYWDDPGTRFARP
                     IRWLLCLWDDEVIPLTFAGCVADRFTSGHRFLGQKRVEVPSVRAYMDLLYDNYVIVDQ
                     DKRRERMTGAIAALEKEIDGKADLTPALVEENLDLVEYPVPFFGTFEDRFLDMPPEVL
                     TTTMAVHQRYFPVHNGQGKLLPLFVGVSNNRATSMAVVREGNERVLRARLSDAAFFWA
                     EDQKRPLASRVEEL"
BASE COUNT        11245 a        16888 c        20178 g        11431 t
ORIGIN      
        1 gtacccgtgg gagagtaggt cgttgccgga aactttttcc ttctcttatt ttttgcgctt
       61 taggggcccc caaaagggac cccttttttt tgtccccaaa accaaaagat caacacaagg
      121 agccgatttt ctcctggact cctaaggggg cgggccttcc ctagtggcga tgatgcctct
      181 tatccggcgg ggctcatacc aaagaagtga ggcagggctg gacgctgcgc agccccttga
      241 tccccgacac cccttccacc aaccgtcgca ctcgccccac gcccccccgc accaggatga
      301 cttccagaca gtgatcttcg tccatgtggg cgtgggaggc gcagaggatg acgtcgccga
      361 aatgatgctg gacgtgggtc agctccgtac aggcgtcgtg ggccatgtgg gcataggtca
      421 cggtgatgct gccgcaaacg tctccatgcc cctcctccca ccgagaatcg gccacgaagg
      481 tgcgcaccag ttgacgaagg gcctccgatc ggttgggaat gcccttgcgg acgatccact
      541 cgtccaactc atccagcagg gattgtccca tggaaacgct gaaacgcacc acagattcca
      601 tgcgtgtcct ctccttccgt ggcatgggcg ttgttggtat actatacccc atgggggggc
      661 gtttaggaga gcctcctttg gggagcggcg gtacgaaacg caaaaaggcg ccccagcgtg
      721 ttttcgcgat cggatcaaag gagtgaagaa aagatgaagg ttgttttggc ccaaaccgcc
      781 cccctgtggg gcgatgcgaa aggcagtggc caggcggtgc tccagagcct ttctcaagtt
      841 cccccctcga ccaggatggt gcttttgcca gagctatgga gttgctccta cgacaacccc
      901 cgcatggaac accacgcgaa ggaaagcccg ggccttctgg acgcgttggc tgcctgggcc
      961 cgggaccgga gaagtggggt cctggcgggg agccttcctt ggcccgtcga tgggggaatg
     1021 gtgaaccgca cttggctgtt aaacgacacg ggaagccccg tggggtttta cgataaaacc
     1081 catctttttc cccttctgga cgaagatcgg gtctttcttc ccggatcggt gccatttttt
     1141 ttcgacgtgg acggcttctc ctggggcagt gccatctgct atgacatccg attccccgaa
     1201 tatatcaggg cgctggccct ggggggcatc gacgtcctgg cggtggtggc ggagtggccc
     1261 cggtcgcgcc ttttggcctg gcgtaccctg gtccaggccc gggccataga gaatcagatc
     1321 tacgtgctgg cgtgcaaccg atgcggcgag ggaggcgggg aggtctgggg ggggcattcc
     1381 atggtggtgg cccccgacgg cacggtgctt gccgagggag gcgatgggga ggagctcctg
     1441 gtggtggacc tggaacttcg aaaggtgcgg gacatgcgtc gcgccctgcc catgtgggac
     1501 catcggcgtc gggagctcta cgaagcagtg agggccatgg aacatcccga ggcctcctct
     1561 ccggagagct cacgataaaa aaggtctcag ggcttcgacg atggcggcgg gggcgtccaa
     1621 cagatcggga agctcctcca gggtgtcgat ggggcgcctt tgtataattc gggaggcccg
     1681 ggccttgccg atgcccggga gggtctggag ggttatgggg tcgcaccgat ttaggtccac
     1741 ggggtagggg acggcggtga gggaacgccc cccgtggctg cataccgcca cgtcccgtcg
     1801 atccccttcc cggaggtcat ccgcccctat tcctaccagg gggggatagc ttcccaggga
     1861 tcgaccgaag gccatgtgtc cccggcgctc ctccacccgc acgtttcgaa tcacggtgcc
     1921 cacgggggcc accccggaga gaaagaccgg atcggcctcg ttacggatcc actctttcca
     1981 ccggcgataa cttcgctccc gcacccggga ggggtatcgt tccaagaggg atgccagggg
     2041 ggcctcgggg aagaccatgc atcggcggat gttgatcctt cggacgagga gtccttcctc
     2101 cagcagcgtt tccaaaaagc gacgattttc ctccagggag tcgtccccgt catcggccaa
     2161 ccccacgagg aagttcagcc ccgggagcaa cgccgggaga agccggggcc ccttgggctt
     2221 tccgccgatc tcgttgacca tgcggacggc aaaaagggcc tcctcggcgg agaccttaag
     2281 atgattccgg tttttcaccc gggcggagac gttttccagc ccgaaggaga ggccgtcccc
     2341 ggcggtgttg tgccggacga tgatttccat ggcctcggcg gcggcgtcgg gaaagcgggt
     2401 gatggtcagg gggttggcgt tgtccgtgtg gagcaccgaa aggcgggggc agcgctctcg
     2461 aaagccctcg tagagggctc ggagcctttc ggggccggga cggcgcccct cgggggtgaa
     2521 actcccccca tatcccagga tgttcgcgca gcgccccagg cgaaaggccg atacgcctac
     2581 gtggtccagg gcctcggcct cttctagcac gtcctccggg ggacgttctt catgggttcc
     2641 gggggaccgt tcggtgcaaa aactgcactg tccctctgag cggtcgcacc cccgggaaag
     2701 ctccatctcc gccataaccc aagggaaatc ggggtgttgg ggcgccagcg ccgccccggc
     2761 gatggcccaa gggcgcagtt cctcataggt gcgggtgagt ccgggcacaa gctggcccgt
     2821 gcgcccccag tgggccaaaa cggcctcggg gtcccctgtg cagaggcaat ccacggccag
     2881 aggaggcagg tctcgggcgc gaactcctcc ctggagggcg taaccatggc ggatgggccc
     2941 tcccagcacc aaagggccac ttcgaggcag ggagctcaag gcctcaagct cccggagggt
     3001 gagggggcta ccccctcggt agcgtccggg gactgtgaga cccgatatga cgaccaccag
     3061 gaccgcctca cgccacaggt cttccagggg ggttcggcgc caggtatcgc aggtgagata
     3121 ggtcacatcc aaccccgcca gggctagggc cccaaaggcg tagcgcacgt agggagatat
     3181 gtagggggga acgccgaagc aggcgggctc gtccacgtat ccatccatta ccagggccaa
     3241 gggcatggtc tctctcctta ttgataattg aatttgcctc tgccacggga ctccctaacc
     3301 ccaaccaagg gcccggcggc ggggagtgag gcttcggccc cccgtgggcc cttttactcc
     3361 tagggagtca gccttcctaa atggaatggc ggcccttgga aggggccttt gccctaaagg
     3421 cagatgtgat acaatgtgtt gtcgtgccga tctccgagag agaggggtgg ggacgtggcg
     3481 agtctccggg aagcggcgtt ggtgagagat acggtggtgt cctggcttcg agaaagggcg
     3541 gccgcctgtg gtatggcggg gggtatcgtg gggctgagcg gcggcgtcga ttccgctgtg
     3601 ttggcggcgc ttttgaagat cgccttcggc gacgccatgc tggcggtgat gttgccttgc
     3661 cacagcgacc cccaggacga ggaagacgcc cgattggtgg cggaagccct tgccctccca
     3721 tgggccaagg tggacctttc ggccccctac gacgctctga cgagggccct agaaatcgaa
     3781 ggtccattgt catccctggc cttggccaac gtcaagcccc ggatgaggat gacggtcttg
     3841 tacgccctgg cgagggatcg ccgctttttg gtctgcggag cttctaacaa ggacgaatgg
     3901 gtggtgggat attttaccaa acacggagac tcgggggtag acctactccc catgggggat
     3961 ttggtgaagg gggaggtgcg ggccctggcg accctcctgg gggttccccg tcgcatcgtc
     4021 gaaaagcccc ccacggcggg gttgtggccg ggacagacgg atgaagggga aatgggcgtg
     4081 cggtatgacg agctcgatga agtcatcctg acgggagagg gggatgaggc cgctaaaaac
     4141 tttgtggccc gtgcccggag ccagtccgag cacaagcgcc acatgccccc cgtgtgcccc
     4201 atcccctcgt gaaccactct tttaggcctg cggcggcgga gcgacccttc gcccgaagag
     4261 cccatcgata aagcggcgtg aggtgttatt ttccaggaac tacagggcgg gatgaggcaa
     4321 gaatcggcat aaaataaagt atatgagaag atttataagg atagctagag ctgtcgcgag
     4381 gcggttttgt tgaattttaa agacattttg cgatgaggtg aaagagcatg tcgggacatt
     4441 ctaagtgggc aaatatcaaa caccgtaagg cagcccagga cgccaagcgg ggggcggcct
     4501 tccagaggct tctgagggct atcatcatcg cggccaaaga ggggggcgga gaccccgcca
     4561 tgaacatgcg cctgaagacc gccatagagc gagccaaggc cgtttccgtg ccgtcggaca
     4621 acatcgatcg ggccatcaag cgcggcacgg gagagatcga aggggcggtc tacgaggaag
     4681 tggtctacga ggtgtacggc cccggcgggg tggccctcat cgtggaggcc accacggaca
     4741 ataaaaaccg cactacgccg gagatccggg cgctcttgac ccggaacgga ggaaccttgg
     4801 gcgaaggggg ctccgtggcg tggaacttcg agcggcgagg gctcatcacg gtggagggtg
     4861 gggtggatcc cgacgaactg ctcctgtccg tggcggaggc ggggggcgat gacgtggaag
     4921 aggacggaga gagcttcacg gtgaccacgg ctccggcgga gctggacggg gtgctggagg
     4981 ctctgaaaaa ggctggatac gtggtggtgg acagcgaagt tcgctttgtg gccaagaccc
     5041 ccgtggatat caatgccaag gatatggcgg gaaagattct caaacttgtc gacgccctgg
     5101 aggagcacga cgatgtccag agcgtggcgt gtaacgccga catccccgat gacattttgg
     5161 aggccctggc agactgatgc ccctacgctg tctcggcatc gacccggggc tcgggaggtt
     5221 gggttttggc ctggtggttc agcgagattc ctccctcacc gccgaaacct acggatgtct
     5281 ggaaaccccg ccgggggagg cgttgcccca acgccttctc cggcttttct ccgacctgga
     5341 tcgggtgatg gaccgtacga cccccgatgt ggtggcggtg gaaaagctct tctttggccg
     5401 aaacgccacc acggcggaga tggtctggca ggctcggggg gtggtccttc ttctgggggc
     5461 ccgtcgggga atccctctgc aggaagtgac tcccaacgag gtaaagatgg gagtcacggg
     5521 cttcgggaac gccaccaagg gccaggttca aggcatggtg caaaggatcc tttctctgga
     5581 tcatctcccc cggccagacg acgccgccga tgccctgggt atcgccctgg cggggattgc
     5641 cctctaccgg tatgacgccc tatcagcgcg gaaggactcc tccggcgacg gatcgcggtg
     5701 atggccttta atacggcttg gaacggatag gaggacagga tatgctgcgc tatcttcaag
     5761 gcaccgtgga aacggtggaa gggcccctgg tggtgctgga tgtccagggt tggggttttg
     5821 aactccaggt ttcccgggcc gcccgatctc gggcccttgt gggggaaagc cttaggcttt
     5881 tcaccgcgct ccaggtttcg gaaagcggcg tggccctttt tgggtttttg cgggagagcg
     5941 agcgatccct ctttcagcgc ctgacctccg tgaagggcgt gggagggaag gtggccatgg
     6001 cccttttggg aactctggag gaagaaaccc tggtgcggtc catactgagc ggagacttgg
     6061 cgaccctctc ctctgccccg ggggtgggga gaaaaacggc ggagcgcctc tgcttcgagc
     6121 ttcgggagcg gctgggatcc tatgccgcag gggaaccggg gatcccggcg aatttcccgg
     6181 gagctctggt gctggaagcc ctagagtccc tgggattttc ccggagcgag gcggtggcag
     6241 ccctgcgtcg caccgcagag ttctcccctc cccccgccac ggacgaagag ctccttcagg
     6301 ctgccctaaa gactttgcga aaatgacgag atcggagcag gagtggggct tcgggaaagg
     6361 agaaagaggt catgtccgat gaccgattgg tccatgcccc cctggggaca aacgaagagg
     6421 atttgcccct tcgccctcag gccttgggag actttattgg ccaagaaagc ctccgggaaa
     6481 aactgaccat atacatcgcg gcggcgaggc tgcgaggtga gcccctggac cacattttgt
     6541 tccacggccc cccggggctg gggaagacca cgctggcggg gatcatcgcc cgagaaatgg
     6601 gaggggagct tcgcatcacc acggggcccg ccctggagcg ctccggagat ctggcggcca
     6661 tcctctccaa ccttcagtcc cgggacgtgc tctttatcga cgaaatccac cgcctgcccg
     6721 cagcggtgga ggagaccctc tacgcaggga tggaggactt tgccctcgac atcgtggtgg
     6781 gcaagggacc catggcacga aacctccggc tcaacctccc cccctttacg ctggtggggg
     6841 ccaccacgcg actgggactc ctcacctctc ccctaagggc tcgcttcggc atcgtggaac
     6901 agatttccct ttacgatgcg gaggaattgg cccagatcgt gctccggggg gcccgatccc
     6961 tgggggtttc cgtggaggaa gatgcggcga tggagatggg gcgccgggcc cggggaaccc
     7021 cccgggtggc cctgcggctc ctccggcgcg tccgggacgt ggcccaggtg aggcaggatc
     7081 cctccatctc ccgagccctc gcccgggggg ccttggaaat gttgggagtg gacgaagacg
     7141 gcctcgacga aggagatcgt cgctttttgc gcacggtggt ggagctcttt ggcgggggcc
     7201 ccgtgggatt gaccactctg gccgccgcga tgaacgaaga ggcccagacc ctggaagaca
     7261 tctacgagcc cttcctaatc caacgagggc tcctggagcg cactccccgg ggacgccggg
     7321 ctacggcggg ggccttcgaa tatctggggc accccgtacc agccgatttt ggcccgaagc
     7381 agctcacact ggagttgggg cggggcgacg agggaaagga agagtaatgg agccatgggg
     7441 gggctgggga agattctggt ggggttgggt gcggccctat tgaccctggg gctcggattg
     7501 atgctgctgg aacggtgggg gatctctctg gggcgtttgc cgggagactt gatattccgg
     7561 aggaagaacc tagtggtgtt tttccccctg gggagcatgg tcctcctaag cctggggggc
     7621 accctgcttc tgtacctgtg gcagcgttgg ggacgctagg gctccagggc agaaagcagc
     7681 ggtaacgcgc ccctcccctg tccccttcgc cagagggcgg ctaaggggcc tacggaaggc
     7741 taagaaagga tgaagtaacg tgaaaaatag ctggcaaaaa ggccttatcc tgggattagg
     7801 gatgatctta gtcctctttg gagagggggt gtcctcggag gcggcggaaa aacccatcgc
     7861 cgtgggagtg ggggtgggga tttcccaggg agttttatcc tcgacaaaac ccttcgtgct
     7921 taaggactcc aaggggaaaa aagtttccgt gggaaaatcg gcgaccttca cgtgctccgg
     7981 aagtcgcctg gagaccccca agggctccct tacccttccc gtgaccgccc aaggcccggc
     8041 ccccatcatc tgggataagc acccctatcg gggagttttg aaactagtcc ccgccgccaa
     8101 agggtgtaat gtggtcaacg tattggggat ggaggattac ctccgggggg tgctcaagat
     8161 ggagacaaac cccgcctggc ctccggaatc tctccgggcc caggccatcg tggcccgaac
     8221 cttcgccatg aaggggttgg ggcgtcatcg aagccagggc tttgacctct gtgccaccac
     8281 ccattgccag gtttatcggg ggatcaatgc ggaggacccc catacggaca aggccgtaaa
     8341 ggccactcaa ggcatggtgc ttgccgtggg aaaagagctt gccgccactc cttatcattc
     8401 ggacagcgga ggggccacgg cgaatgtggg agacgtttgg ggggggagcg tgccctatct
     8461 caaggcccag gccgaacccg tggcctacac gtcgccctat tctagctggc aactggttct
     8521 ttccccggcc caggtggagg aggccctggg gaggatcgac gtgcgcgtgg ggacggtaac
     8581 gggaatgacc gtggcgcaaa aggacccctt cggccgggcg gtacgcctgg agatctgcgg
     8641 gacgggggga aaagccgtag tgtcgtccca caagtttcgc atggccgtgg gagcctccgt
     8701 cctgcgaagt acggtctttg agctgactgc gggcaccacc gagagttctg gggcccccat
     8761 gccggaagaa accccttccg gcgaaggaga agaggcctcc tacttctccg agagcgtgga
     8821 agagggggat ctgctcattg cgatgacgcg ccagggagcc ttcaccacgg cggagctcat
     8881 ggacatgctc atgcatcccg agacgcgggc ccagcatatg gcagaggccc tggcccgggg
     8941 agggaggggc gccaaaactc ccccccttcc ctccaaggct cctgaccgga gggccccagg
     9001 gaagggcttt gtcttccgag gccgggggtg gggtcacgga gtgggcatgt cccagtgggg
     9061 agccaaggcc ctggccgacg gcggatggaa agcggagaaa atccttgggc actactatcc
     9121 aggcaccaag cttcgaagcg cttattaaag cccagagagg gcgggggcgg ggagaaactc
     9181 ttccggcgac agacggtgac gcggagctct ttgccacggc gtcctacgac tacgacctgc
     9241 cggaagagct gatcgcccag aaccccgccg agccccggga tgcgtcccga ctcctggtgg
     9301 tcccccggga gggaggagag ctgggtcacc gacgtttttc cgacctcccc accttgctgt
     9361 gccccgggga ccttctggtc cttaacgaca cccgagtgtg gcacgcccgg ctcctgggcg
     9421 ccaagccctc gggggcccgg gtggagttgc tcctgctacg ccccctggac gccgaagaga
     9481 ccctgtggga ggccttggtg cgtcccgggc ggcgcctgaa tcctggggcg gaggtgatta
     9541 tcccaggagg aaggggcgtg gtgggagagc gccggggcga agggctgcgg gaggtgcgct
     9601 tcgaattgac ctcggggacg gtgcgagagg tcctggcggc ggagggccgg gtcccccttc
     9661 ccccgtacat ccgttccacg gaggcccctc cggggcgata ccagaccgtg tacgcccggc
     9721 aggagggatc cgccgccgcc cccacagcgg ggcttcattt caccgaaggg ctcttggaga
     9781 ggctggaaga aaggggtgtg gagatctcct tcgtcaccct tcacgtgggg ttggggacct
     9841 ttcgccccgt ggaaagcgac gatgtccgcc gccacgtcat gcacgaagaa cgctgctttc
     9901 tgccggaggg gaccgccgat gccgtggccc gcgcccgggc gcggggaggg cgggtggtgg
     9961 cggtgggcac cacggtggtg aggacgctgg agacctttgc ctcggacgat ggcaccctgc
    10021 ggccggggac ggcggacacg gggcttttca tcaccccggg atttcgattt cgggcggtgg
    10081 acgccctggt gaccaacttt cacctccccc ggagcaccct tctcatgctg gtctccgcct
    10141 tcgcgggata cgatcgagtg atgggggcct atcggaaggc cgtggaggat cggtaccggt
    10201 ttttttcctt cggagatgcc atgtttatcg cctaggatcg aggcccatac cccttttttc
    10261 tccaaacgaa acgaatctct ttgataaaac gcccagagat aacccgagga ggaggagaaa
    10321 attgcgttgg ttgaagctcc atccggaaga ctaccgaaaa atgccttgga aaaacggcgg
    10381 gggagtgacg gaagagatcg ccctggatcc cccggggggg tcatgggagg gaccttcgcg
    10441 gattctctgg cgcgttagta cggccccggt ggcctcgtcg ggccccttct ctatttttcc
    10501 cggctacgac cgcctgctgg cgatcctcga cgggggggat ttggagctcc gggtggaagg
    10561 aaaagagccc gagcgccttt ctcctcgggg gagggtggga tcctttcccg gcgatgtccc
    10621 cacagaaggg atcctcttgg gatctccttg tagggatttc aacttgatgg tggaccgggc
    10681 ccgaggtgag gcgatgctac gctttgtttc cctcgacgag gaagggactc tgcgaagcaa
    10741 aaagagcgtg gggatcctct ttgccgctca gggggaggtg ggggggcggg gaccttctct
    10801 cgaaggggag ccctttgttc tgtccccggg ggattgccta ctgtggcgcc gagaagacgg
    10861 agatcccgaa gccttctttg agctacgggg ctccggatgg gccctctggg gagagattat
    10921 aataagaacc taatcaattc ttccgggggg ttcaagggta ttaaagtttc ttggagttcg
    10981 acgacctgaa gaacctctgg aacgacgaga gatctggtct tagcactttc gccttgcctg
    11041 tgcgccggag acatattata ctaaaggcgg cgctggctca tggggagcgg cgcggcggag
    11101 ggaagaggcg tcagctaagg gactatatca tcaaaggagg ggtttgtgct catgaaacgt
    11161 tatggagtcg cggtattggt ggttcttatg gtgttgggca cggtggcggg ggcgggagcc
    11221 aaggaattct tctccatcgc cacgggcgga accacgggga cctactatcc cctggggggc
    11281 ggcatcgcgg agatcataaa cagccacgtg cccgacgtgc aggtcacggc ggagacagcc
    11341 aacgcttcgg cggccaacgt caacctcatt ggcagtggcc agatcgaaat ggccttcgcc
    11401 cagaacgaca tcgcctactg gggatggaag ggcgaaaaca tgtttgaagg caagccctat
    11461 gcgaacatac ggggcgtggc cgtgctctat ccggagcacg tgcacctcat ttccatccag
    11521 gacgcgggca taaaggcctt cggagacatc aaaggcaagc gcctttccgc cggggccccg
    11581 ggttccggag tggaagggga cgtgcgggcg atcttcgatg tggcgggcct gacctacgac
    11641 agcgtgaagg ccgacttcct cgactttgcc aataccaccg ctcgcttcaa ggacgggcag
    11701 ctggatgcgg gtttcgtggt gggagggtac cccgtggcgt ccatcatgga cctggccacc
    11761 accgccaaga tcgacctggt gaactttgac gaagccttcc tggatcagct gatcgccaag
    11821 tatcccttct tcgccaaggg cgtgatcccc gccaacacct attccggcgt ggaccacgat
    11881 acggtgaccc cccaggtcat ggccatgctc atctgccggg aagacctctc ggaggagctc
    11941 gtgtataact tcactaaggc gttctgggag aacgtacggg agctggataa ggtccatgcc
    12001 aaggccaagc tcatcaccct ggagaccgcc ctgcagggcc tttccgtccc cctccacccc
    12061 ggggcggcga agtactacgc cgaaaagggc atggccatcc ccgaaattaa gtaggttacc
    12121 cgtataggcc agcggagagc ccaagcgaag gccccggaga tctccggggc cttcgtcgtg
    12181 gtataacctg ccggaggtcc tcccggggcg gagtaggccg cagaaggggc ccatgggggg
    12241 cggcgctaaa gcccgataag ggtttagatt cggcgacggt tcaaaagctt tcgaccattc
    12301 gatttcgcca tacgctgatt tgtcgcagaa ataggggccc cgccccaggt caaaaggtcc
    12361 agaagcggtg gcccttgcgc ggaggggtct cgggaagatc tctttccttc gccgctgcgt
    12421 cccgggcctc ttggtgggcc cgccgtcgcc gttggtctcc ttcgacggat tccccctccc
    12481 tcccgggggt gatcagggag gggcgatggg gggtcgactc ttgaagggcc gcgtcttcct
    12541 cggccctccg gcgctccccc cggcggtcct gggcttctcg ggagagctgc tgctcctgag
    12601 ccagggtctc aaaacggtcg tgccgtctct cggcctggtc ttttttctcc agatctagta
    12661 attttttctg gcgttccttt cgctggcgct cctgggaggc ctggacgatc ctctcgtggc
    12721 gctccagaat ttggtcccgc tcgtgggcga cggccccttg ggtcttttcc caggcctgac
    12781 ggagcgtttc cggcgtgacg tcttccggtt ccttgggcac gatggggcgc caggtcgtcg
    12841 gatctacggg aatgttttta gggtcaacgc gcatgggaaa ggcccccttt ccgcagggta
    12901 aaagcaccgg ggttccccga aagaaaaccc cggcctcttc gtcagtttcg gcggtgcgga
    12961 ccctttagat aagccgtcgg cgccccgagg ggtgcctttc gatagggaag agcatgggct
    13021 ttgcgggggt gttagaaagg cgtcgatcca ggggtgccgg gaccgtcccg acggaggctt
    13081 tgggtagggg aatttagcga ttttccccct ccggggcgat gaaccgacgg acttcttcca
    13141 cgagctggtc tcgggcaacg ttgtcgtgaa cgaggtcccg ggtgtccgta tccagcacca
    13201 tgtaggaccg cattcgttta ttccggggaa ggttggcgat gaaccgggaa tagactgagt
    13261 ggagctcctc taggaattcc tctccagagg gttcttccga ggcaccgggg gccttcccca
    13321 tgcgcttcat catggtgccg aaggagcagg tggtccagat aaatacccga ggcataacca
    13381 ggtagctcga aagaaattcg aagagttgga cgtacatctg atactgccct tcggagaagt
    13441 agcggagttt gtggtaggta tcggcgtagt tcatgtctcc aaaaatgcta cgatcgagga
    13501 taaagtcatg ggattccgag gccatgagat attgggcaaa gcgggtggtg agaaaattga
    13561 gctgcatggg gaatgaccag cgctggtcgc gcccgtagcg ttctagcagg gcgtactgcg
    13621 tccggaactc ctcgccgatc tgctggagcc ccagacgttc cgtgaggagc tttaccacgg
    13681 tggttttccc gctggcggtg atgccttcca cgatgacttg tacctgtccc acacgtctca
    13741 cctcgttttt agtcaacctg cggggggatt tattcccccc acggccctat ggtagcacac
    13801 ccggccgata aaatccttcg gccggtcgtc catgcctttg gattgctacg ggctcgcctt
    13861 tttgatctac gcctctcccc cggcgaaaaa cgcctcggga aagagggggg agggaaaaat
    13921 ctttgccggt ctgccagaaa gcccttgacg tctttggctg gagccgatac tattcccttt
    13981 gtgctttttg cttgggccat tcccatggag gtgcaaaaaa atgaagaaag atatccatcc
    14041 taagtatggt gtgtgtgcgg tgaagtgcgc ctgcggcaac gccttcgaga cctcttccac
    14101 ccataagggc gagatgcggg taagcgtttg ctctgcgtgc catcccttct acacgggtaa
    14161 gaagggcaag atggtacagg cggggcgcgt ggagaagttc cagaagaaat acgccgcctt
    14221 cagtcagcaa caggcgcagc agcagtagct ttccgtgcag ggttgagggg gggccgacgc
    14281 tccccctctg ttgtcgtgtc ctccgatggg aggttgcgag aaaggagggt gtcgtggggt
    14341 gtcaggtgtc gctcctctct tgctccccgg atccggaccg tttggtggcg gcggcggctc
    14401 gcctttgcta tagtcctgct tccgcccggg atctggcgga agttttcctt gacgaagggc
    14461 gcgttcgacc tttcctggag aagctccgcc gtagcggcca tctttccacc ttcgagcatg
    14521 cgtctttctc ctttgcggtg gacggcataa gccgggtgtg ctcccatcag ttggtgcggc
    14581 atcggctggc gagctactcg cagcagagcc agcgctatgt ctcccaggag aaggccggag
    14641 tggtgatgcc tcgaagtata agggagcacc cccgggcccg agaggtcttt gaagaggccg
    14701 ttgcccaaac ccttctggcc tacggccgcc tcggcgccct gggcattccc cgggaggatg
    14761 cccggtatgt tctgccccac gggggagaaa cccgcatgat ggttaccatg aacgcccgag
    14821 agctactcca tttcttccgg ctgcgcctct gccgccgggc ccaatgggag attcgggacc
    14881 tggctcggga gatgttgagg ctggtacggg aggtggcgcc gggcctcttt gccgtggcgg
    14941 gccccggctg tgtggagaca ggggcttgcg gcgaagatcg cccctgcggt aggccttttg
    15001 tggatacgga ggatgtgctg gcccatgcgg tggattgatc tgacgacgtt tttctggggc
    15061 cttttttctc tgggggtgga aaagatcccc gtgggaggtc aagcggtgat cgagggggtg
    15121 ctgatgcgcg gtccccggcg atgggggttg gcggtgcgcc gtccggaggg agacatctgg
    15181 catacctctt gggggctggc cccctgggcg gcccgaggag tgtggaaatg gcccgtctgc
    15241 cgggggctgg cggtgatggg agaaatgctt tccaccggaa tgaaagccct ctcccgctcg
    15301 gcggagatcg ccctgggaga agaggagaaa ctaacgggaa aagacctggc tctctccgct
    15361 ttggtggctc tgggaggggt ggtgggactc ttcctcctcc ttcccatgtg gggggcggat
    15421 ctcctgactc cctggacggg accttcgctt gtggtgcgtc acgtggtgga aggggtgcta
    15481 cgggccctgg tatttatcgg gtatctggcg gtcattggcc tgtgggggga catccggacg
    15541 gtattcgcct accacggggc ggagcacaag accatcaacg cctacgagaa cgacgcgccg
    15601 ctgacgccgg actccgtgga gaagttctcc cgaattcacc cccggtgcgg cacgtcattc
    15661 ctattggtgg tggtggtggt gagcatcgtg gtctttgcct tggcgggaaa tggccccctg
    15721 tggtggcgct tgtcctcccg ggtggtgctc cttcccctgg ttatcggcct atcgtatgag
    15781 tttatccggt ggagttgtcg ttctccccgc gtagggtccc tggttatggg gcccgccctg
    15841 tcgttgcagt atctcaccac acgagaaccg aatcgggacc agttggcggt gggcatcgcg
    15901 tcactccagg cggctctgga agagcctccc tcggaggact cggggcgaga ggcgccgccg
    15961 ggaggggaag agcgctgatg gagataacaa aaaaacttga agagattgtg gctctctacg
    16021 aggatttgga gcgtcgcatg gcggattccg ctgcggaggc ttcgccccag gagttgcagc
    16081 aactggcaaa aaagcactcc gacctccagg aggtggttca tgcctttcgg gactaccaaa
    16141 ggcgccagaa gaggctcgcc gagtcccgcc agttggcgtc ctgtggggac gaggagatgg
    16201 aagagctggc tcggtcggat gtcgaggagc tggaagaagc tctgatccag gacgaggacc
    16261 tgctccggcg cctcctgttg ccccgcgacc ccaacgacga aaaaagcgtc atcatggaga
    16321 ttcgaggagg ggcgggggga gacgaggcgg ccctctttgc cggagatttg ttccgcatgt
    16381 acacccgctt tgccgaacgg caggggtgga gggtggaagt aatgacgctc agcgaaacgg
    16441 gcatcggcgg aatgaaagaa gcgatctttc gcatggatgg cctgggggcg tacagcaagc
    16501 tcaagtttga gagcggggtg caccgagttc agcgggttcc cgttaccgaa gcctcgggac
    16561 gcattcacac ctccaccgcc accgtggcgg tgctccctga ggtagacgac gtggaggtgg
    16621 aggtgcgtaa cgaagacctg aaaatagata cctaccgggc cagcggggcc ggagggcagt
    16681 atgtcaacat gaccgactcc gccgtgcgaa tcacccacga gcccacgggg ttggtggtga
    16741 cctgccagga cgagcgatcc cagcttaaaa accgagtaaa agccatgaag ctcctgcggg
    16801 cccggcttta cgatctggag cttcagaagc aacagaacca gcaggcggcg gagcgccggg
    16861 gtcaggtggg cacgggggat cggtcggaac gcatccggac ctataacttt ccccagaacc
    16921 gggtcagcga ccatcgcata gggctcacgt tacacaagtt ggacctgctg ctagagggcg
    16981 atctggatga gatgatccag gcccttacca tggccgacca ggccgcccgg cttcagcaga
    17041 gcgccctgga atgacggtgc cgtgcgggta gacgaaggac ggcgctgggg ggcggagatc
    17101 ctcgccgccg ccggggtggt ggacccccag cgggacgtgg accttttgct ggaagaggtg
    17161 ctttaccgac cctgggccca tatcctggcg cacccggaag ggctcctatc tccttcggag
    17221 gaggatcgct ttcgggagct tctgtcccga cgggcctccc gggagcccat ggcctacatt
    17281 ctgggagaga agggcttttg gggacggaac tttgctgtgg gccctggatg cttgatccct
    17341 cgggatgaca cggaggccct catggaaacg gtcctcgggg tctttcccgg gggccttttc
    17401 gtcgattggg gtacgggaag cggatgcctt gcggccacgt tggccctgga gcgtcccgcc
    17461 agtcgagggg tggcggtgga ggcctcgcct cgggccctgg tttgggcgcg gcggaacctg
    17521 cgttccctgg gggtgctcgg ccgggtcgac ctggtccacg ggaaggcgcc agaggatctt
    17581 tcccttgccc ccggggttgt ggacctggtg gtgagcaacc ccccgtacat cccctccctc
    17641 cgtctggcgg gattgatgcc cgaggtgggg cgcttcgagc ccgcagaagc tctggacggg
    17701 ggcgacgacg gaatggattt ctaccgcatg atctgcccct gggcttccac cgtgctgaag
    17761 gagggaggat acctggcggt ggaagtggga gacgaggccc aagcggcgtg gctgcgagac
    17821 cacggggggg catctttgcg gtggcagcgt ttcttttacg atttgaaagg cgaggtgcgg
    17881 ggagtggtgt ggaaaaaaac ctccccggtg gcgtacaata ggcccgggag ctcttccctg
    17941 cggctccagg gggccggaga gagtcgaacg tgaaggagga aaacgaagtg gaaaaacggc
    18001 aactggtcat catcggcgcg gggcctgcgg gattgacggc ggcgatttac ggcaagcggg
    18061 cggggttgga cgtgttgctc ctggagaagg gagtccccgg ggggcagatc aacgtcaccg
    18121 acgagatcga gaactggccc ggcgtggaac acgccagcgg cccggagctg gcggagacct
    18181 tccgtaagca cgcggtcaag tttggcacgg aattccgaga ttcctcggtg cgaaaggtgg
    18241 agcttcgcga cggtgccaag gtggtggtca ccgaagaggg agatatcgag gccgaggccg
    18301 tgatcctggc cacgggggcg agctacaaga agatgggatg ccctggggag gcggaattca
    18361 ccggccgggg ggtgagctac tgcgcggtgt gcgacggggc gttctttgaa ggggagacca
    18421 tcgccgtgct cggaggcgga aattccgccg tggaagaggc ctgctatctg acccaatttg
    18481 ccgaaaaagt ctatatcgtg caccgtcgag actccttccg ggcggaccgc gtggcggtgg
    18541 agagggccct cgccaacccc aagattgagc ccgtgtggaa cagcgtggtg gaggccatcg
    18601 aaggcggcga catggtggag aagctggtga tcaaaaacgt caaaaccggg gagaagagcg
    18661 aactccccgt ggcgggggtc ttcgtcttcg tggggagcgt ccccaacgac gaaccactca
    18721 agggcctcgt ggcggtgaac aaagcggggt ggatcgagac caacgaccgg atggagacct
    18781 ccgtagaggg gatcttcgcc gccggggacg tgcgggataa gttcctgcgc caggtcatca
    18841 ccgccgccag cgacggcgcc gtggccgcca tggccgccta cgggtatatc tccgagcagc
    18901 tccacctgag gagcaccttg ctggaccccg agcgcgtggt ggccctcttc acctcgagcg
    18961 tggacgaacc tcaggtgcgc ctgacggggg ccgtggagga gtggtgcgcc acctcgggga
    19021 aaaagatcgc cctggtggat ggctacaaaa acgcccgcat ggtggaacac ctgggacttg
    19081 ccatccttcc ggccctggtg gagcttaaca agggtgtggt ggtaaagaaa gcggcggtct
    19141 cttccctgaa agaagttcag gatttctgct aacccttgag ggggcgcttg tttttggagc
    19201 gcccccgacc ctttgggggc cgaggggggg atcgcgccct tcctagggtc gtggtcgatt
    19261 tcgtgaggag gtgggctcca tgattgttac cgtaaccctt aatcccgccg tggacgaagg
    19321 atatttgctg ccggaattcc atcccggcgg gtggttccga tcggatcgac gggtggaacg
    19381 gacccccgga ggccggggga tcaacgtatc catgacattg cagcagctgg gctacgaaaa
    19441 cgccgccatg ggctttctgg cggggttcaa cggcgagtac gtccgagacg cgctccgccg
    19501 ggcgcacatc accacgaact tcgtccacgt gtcgggggag acccgtacga acgtttatat
    19561 cgtggatgaa atcggacata tcgaaactgg gctggcggaa ctcggcccct acatccccga
    19621 agaggccttg gctcgctttt tctccaacta cgagcgcatg ctccatcgtt gttccatggt
    19681 atgtttgggg gggtcgctgc cccccggagt gccccaggat atctaccgag acctggtcaa
    19741 tagagccaaa gatcgaaaca tcgccacctt tgtggatgcc gagggcccgg ccctcatggc
    19801 ggtgctggag gcggtgccca ccgtggtgaa agtggatcac cggtacatgt cccgagtggc
    19861 aggtatcgcc accacctccc tggacaaaat cattgagatc gtctcccggg tccatgaaaa
    19921 gggggtggat tacgccctga cctcctatcg aacctacggt tacgtcttcc acacccccca
    19981 gggggtcttt ctcgcggaga tggaccgcaa gggcgtggtg tccctctttg ggtccagcga
    20041 ggccctcatg gcggggctgg tggtggcccg ggcggagcgt atgccggtgg atgagaccct
    20101 gcgctttgcc atggcctgtg cccaagagga ctctttccac gtggaaaagg gcgttcgggg
    20161 tcgtcgagaa gtcgaggcga tcatggacca ggtccacctg gaaaaattgg cctgaagtcg
    20221 gatggggggg cgtgatccat gcagatcgga gacgtcatag atcgggattt gacggcggtg
    20281 acgggggata tggcggtggg ggtggtggtg gagatcctca ctcgtcatcg cctttcgggg
    20341 gtgcccgtgg tggacgatga agggcgtgtc ctgggcttcg tgagcgaaga agacatcgtc
    20401 cgggcggcct tgccgggcta ctttgagtat ctctccaatg cctcgaccat acccgacatc
    20461 ggtcaattcc aggcgcggct ccggcgcatc ggccgagagc ccgtgtcgaa gtacatgaac
    20521 accgccgtga cggtcttcaa agaagatgac agcgactttt ccgtggccat ggccatgata
    20581 tcgaaaaatg cccggcgaag ccctgtggtg cgagacggag tgctcgtggg ggcggtaaac
    20641 cggggggacc ttctcaaacg catcctgacg gacggcgaaa aagaagagga ccgctgaccg
    20701 ggagatcatg tctcgggggc cgagtgccga tactggagcg tggggggagg ggagaatatg
    20761 gacgacaaac aggtccggca tgcccaggaa acctatagaa taaaccagat caaaaccgcg
    20821 tcccgggagc agttgctcct catcacctac gatatcggca ttcaggcgtg taagatgggc
    20881 gaggaggcca tgaggcgagg gaacgtggag gagaccaacg aggcccttca gcgggcccag
    20941 tcggtggtgc gggagctcat ggtgaccctc aacgtggaag tgggaggcga cgtggcgaag
    21001 cagctcatga acctctatga cttcctgtac tacgagctcg ttcacgccaa cgtacaaaaa
    21061 gatatggacc gcctggtgac cgtgcggacc atgttacagg agttgcgtca gacctggcag
    21121 gaggtcgccg ataagcttca cgccgaagaa gaagaaccga tggcgttacc ccaggaggcg
    21181 gtccccgggc cgaaggccgt gaacatggtg gcggcagggg gacgtccttt gctgcacgga
    21241 gggttgaacg ttgctggttg atcttaaaga gcgcctgaaa ggcctcttga cccgggagct
    21301 tgccctctac gaccggctcg agtccaaagt ggccgaggaa gagctctcca tccagacgga
    21361 agactgggag gggctgttgc gggcgttgca ggaaaaacaa gccctcattt ccgagcagga
    21421 gactcttcag gagtcctgga ggtcctgtgc ggtgctcctg gaggtggacg gaggacggga
    21481 gagtgcggag ttctgggaca ccctggctcg gaagatggga cataattcgt accaggaaat
    21541 ctccgttttg gtggagaacc ttcgagaggt ggcccggagg accctggagc gagagagggc
    21601 ggtgcaggcg acccttgaag cccacctaga ggctctgcgg gcccggatgc gccagttcca
    21661 gaaggggaaa gaagccttta agacctatgc ccggacgggg ggagcgagct ctgctcccta
    21721 gagagggcga tcttttgggg gcgtcgtttt gagaaaatgg ggaggccggg gccgccgggt
    21781 catggccgcc cttttgctct gcctactttg ggggtcgtgg gccgaggccc tcacctccga
    21841 cgatgaaaaa gagattgcct tcgggtgcaa gatcgctgag accgtcgacg cctcgggcca
    21901 gcgtgtgggg aatccagcgg tgctcgccag gctggcgatg gtgggaaagc gcctcacccc
    21961 cttcttgacc cgtgaccttc ccttcgagat ccgttgcatc tccctttccg gagatcggcg
    22021 gcccaatgcc ttcgcccttc cggggggtat cgtctacgtc accgagtcca tgctggattt
    22081 cgtccgtagc gacgcggagc tcgcgggggt cttggcccat gagctggtgc acaccgacag
    22141 gaagcatggc atgatccaat ctgcccggaa ctcccggctg tccttgctgt cgctcctggt
    22201 ggcggtggca agccggggag aagtcgccgc catggtcttg gcgaacctgg ttcaggtcgc
    22261 catcatgggc aactacagcc aagacctgga agaagaggcc gatcgccaag gactcgacct
    22321 gctccgaaag ggagagtacg atccagcggc catggtgacg ctcttggaac ggctggagga
    22381 agagcgcctc aaacacccct atttggatcc cggagtctac caggatcacc ccgataccat
    22441 ggatcgcgtc aactacatca tcgagaccat gagagacctc cggtggcccc tgcaccggaa
    22501 gggccctctg aacctcctgc gggtgcgggg agaacaagaa gggacctggt ggattctgcg
    22561 ggtagatgga gacgaggtgt ggcgttgtcc agcccaggag tggtcctccg ccgaccgacg
    22621 atccctgacg gacgccctgg acggagcact gcagctagag cttcccccgg cggatctaga
    22681 ggtggtctcc catccccagg ggagcttttt gcgcctcggt caagtgcgtc tgctgggcga
    22741 accccttccg ccagggagct cgtcgctcaa ggactttcgc caggcgatcg tacgagtcct
    22801 tgacggggcc cgccgtaaac accccttggg tcagtactta aaatagatct ccatccggag
    22861 gactccccct atctccgggg agagcgagga tggcgattaa aagttgcctt ctactgtgct
    22921 cccatgccgg gcaagggctc ggcatggttt ttttgtgctg aagggaagaa catcgtggga
    22981 aaattgcttc ccgaggggta tcctgccata atggccccag gtcgcagatc ggccggagtc
    23041 cgagaacccc tttccggtta tttttttgcg gcgcaaagga ggcccacgcc gtgggttatc
    23101 tgaccctgta ccgaaaatac cgtccccagc gtttctctga tgtcgtcggc caatctgcag
    23161 cggtggacgt gttgcggcac gccgtagaag agcgtcgcat ggggcacgct tacctgttct
    23221 cgggacctcg gggatgtggc aaaaccagcg tggcccgcat agtggccaag gcgttaaact
    23281 gtctggatct ccaggaaggg ggagagcctt gcggggcatg ctcccaatgt catgccattg
    23341 tttccggcga aagcatggac gtgctggaga tcgacgcggc gtcaaaccga ggtatcgacg
    23401 aaatccgaga gcttaagagc cacgtgggct tggccccatt ttctggtaaa tttaagattt
    23461 atatcgtgga cgaagtgcac atgttgacgg agcccgcctt caacgccctc cttaaaacct
    23521 tggaagaacc ccctccccac gtggtgtttc ttttggccac cacggaaccc cacaaggtgc
    23581 cggtgaccat ccggtcccga tgccagcaca tccccgtgcg gcgtcttgag ggccatacct
    23641 tggtggccct gctccgggag gtgttgcggg ccgaaggggc ctcgggggat gaggatgctc
    23701 tgtgggagat cgcccgccag gccgatgggg gggctcggga tgccctctcc ctgctggagc
    23761 aggccctggc cctggggcgg gggcaccttt ctcgggaagg ggtggatgac cttctagggg
    23821 gggcgagccg cagcgagctg gaacaggctc tggtcgtact tcggaaagac cccgcaggat
    23881 ccatggcgaa gtttcacgcc atgctttctc ggggggcctc cctggagcgc ctcctggacg
    23941 gatactttct cctactaaaa gacctatggg tggtctcttc ctggtccgcg aaggccctgg
    24001 aaggaggggg cctttctgac cgggagcggg cctttgtgga ggccgaagcg ccccactggg
    24061 accggaggcg cctgtggagg ggtatgcaat tttgtgcctc ttggcttccc cgtctccgct
    24121 ccggggtccg gggagaggtg gtgctgggcc ttttggtggg caccatggag tcaggggggg
    24181 ctccggaggg cgaggagagg cccacccctc gtcctccgct tccttcggaa aaagaagaat
    24241 ccaacgaaag gccctccgcc gcatccgata agggtctacc ccccccgggc cgcgacgaag
    24301 ggcgcgaggg acgaaagccc ctccgtctcc cgcaggaaga ggctcctggg gggggcatgc
    24361 cccccccttc agaagggact ccggaggtcg cagcccagga aaaacgcgag gcctttcccg
    24421 aggctccgtc gacgccagac ccctctccgg aggggtggaa gcggatggcg gccgccctag
    24481 aaaggcaaga cccgggaatc ggggcggccc ttttggggct ccgggccctt cgggacgaag
    24541 accggctgat actagatgtc ccggaggatt ttcccttggt gtttcatgcc ctgggagggg
    24601 ggaaggggag tcttctcctg gttcgagccg cccatctctt tctcggcctt cggagtgtgg
    24661 agttgcgctg gagggatcac cgtcgcgtct tccccggtgg aacggagggc gaaagagagc
    24721 aagaggaaaa gaaagaaggg gaaaccccct cctggcaatc ctttatcccg gatcgagtgc
    24781 gggaaatctt gccctccggt ggaccggaga atctgccaac cccgaaagcc tcggaacctt
    24841 cggcgttgga gcagatcctc tcctgtgccc cggcggaggt gttgatggtg cgtcgggagc
    24901 tggggtcgga ggaagaagac gcggagatcg ctggacatcg ggaggacgaa gaatgactcg
    24961 acccttcgtg caccttcacg tacacacgga atacagcctt ttggacggag ccatccgctg
    25021 cggcgacttg gctcgtaagg cggcggagta tggtatgccc gccgtggcca tgacggatca
    25081 tggggtgctc tacggcgcgg tggagtttta cgaaaagtgc tgtcaggcgg gagtgaagcc
    25141 catcttaggc tgcgaagtct acgtggatcc cgaggggcac acccggcggg acaaggggtc
    25201 ccggaacaac cacttggttc tgctggcaga agacgatgcg ggatatgcca atctggtaaa
    25261 actggtctcc attgccaaca ccgacggatt ttattacaaa ccccgggtgg accacgacct
    25321 gttggcccgc tacgcccggg ggctcattgc ctcgtccgcc tgtctgggag gcgagatccc
    25381 cgccttgatc ctggagggaa acgaagaggg agctcggcag agggcttgtt tttataacga
    25441 cctttttggc aaggggaatt tttacctgga gatccagcac aacgccatcc ccgaacaggc
    25501 ccgggtaaac aaggccctga taggcctttc ccgagagacg ggtattcctc tggtggccac
    25561 caacgatgcc cactacctta acgccgaaga tgcctcttgg cacgaaatcc tgctctgcgt
    25621 ccagaccaaa agcaccatga gcgaccctaa gcgataccgc tttggggcgg cggacttcta
    25681 cttccgctcc cccgaagaaa tggatcggct cttcggcgct gaggtctccg aagccctgga
    25741 cgccacggtg gccatcgcgg agcgttgtaa cgtcaagttc tgttttgggg aataccgcct
    25801 gccgcacttt gccgtccccc cgggggagac cctggaaagc cacctggagc gcctgagccg
    25861 cgcaggattg agggatcgca tgggcggggc gccgtctccg gagtacatcg agcgcctgga
    25921 gtacgagctg ggagtcatca atcgcatggg ctttgctgga tactttctca tcgtggccga
    25981 ttttatcaat gcggccaaaa cgagaggcat ccccgtgggc cccgggcgag gttccgccgc
    26041 cgggtctctg gtggccttcg ccctccgcat caccgagctg gaccccatac atcataagct
    26101 gcttttcgaa aggttcctca atcccgagcg aatcagcatg cccgatattg atactgatct
    26161 atcggacaaa cgtcggggcg aggtgttgga ctacgtcgtc cagaagtacg gccgggacaa
    26221 cgtgtctcag atcatcactt tcggccggat gatgagcaaa ggagcggtgc gggacgtggc
    26281 ccgctctttg gacatgccct acgcggaggc ggatcacctg gccaaattgg tgccggacgg
    26341 agttaaatcc cttcaggagg cccaggagaa atctcccgat cttcaggctc tcgtccagag
    26401 cgatcccctg gcgcgaaagg tcttggacat cgcctcccac atcgagggct tggctcgcca
    26461 ctcctcgcag cacgccgccg gagtggtcat tgccccttgc ccggtgtcgg acgtcgttcc
    26521 cgtgtgtcgc ataggagaga accaagtcgt cacccaattc gccatggaac ccgtggaaaa
    26581 gcttggcctc gtcaaaatgg actttttggg ccttcggacc ctatcccttg tggaagacac
    26641 gctggccaac ataaaaagca acgggaaaat cccccccgac ctggagaagc tccctctgga
    26701 cgacgaaaag acctatgccc ttttgcagca ggcggacacc ctgggagttt tccagctcga
    26761 atcggggggc atgaggcagc tcctgcgtcg gatggccccc gatgcctttg aggacctggt
    26821 ggcggtgctg gcgctctacc gaccgggtcc actggaaagc ggcatggtgg accagtacgt
    26881 ggagtgtaag catcgacgcc gggaggtgga gtacccccat cccaagctcc aggaagtcct
    26941 ccgagagacc tatggcgtga tcctttacca ggagcaggtc atgcagatcg ccgccgtgct
    27001 ggcggggttt tcccttggag aggccgacct gttgcggcgc gccatgggga agaaaaaaga
    27061 agaggtaatg agggaacaac ggggtaagtt cgtggccggg tgcctgaaaa acggcgtggg
    27121 agagagggtg gcggaggaga tcttcgacgt catccagaag ttcgcagggt acggattcaa
    27181 caagtcccac agcgcggcct acgccatgat aagctaccag acggcctatc tcaaggccca
    27241 ctatcatgcc gagttcctgg ccgctgtctt gtccagccag atcggctctc gcatcgacgt
    27301 cctggcgcgc tacgtgcgaa gtgtgcggga tgcgggggtc gccgtcttac ctcctcacgt
    27361 gaacgaatcc ggcgccgact tcaccgtggt gggtgacgcg attcgcttcg gcctcagcgc
    27421 catatctaaa gcgggggacg cggcggtgga ggccatccta tcgtccagga ggaaaaaggg
    27481 tcccttttcg tccctctggg attttctgaa acgcgtcgat acccgagtgg tgaatcgggg
    27541 agtggtggaa aacctcatcc ggtccggggg atttgatggg ctggggggca attccaggca
    27601 actattggaa gccctgccat ccctatcgga tctgttggcc cggcggtcct ccgacggagg
    27661 gcagggatcc ctcttcgggg cttcggggag cgaagaagaa gaacgccctc ctctgcccga
    27721 cgtgcccgaa cccactcctc gcgagcgact ggagatggaa aaagaaacct tggggctata
    27781 tctttcgggg catccggtgg acgattacgt ccatcgcctg cttcgtttta caaactgcgt
    27841 catcgccgat ctgtcctttt ggaaggcacc ggattcgctg gtatcggtgg gaggcatgat
    27901 caccgagcgt cgagaggccc tcacaaaaaa aggcgatccc atgggggtcc tgatcctgga
    27961 agaccccgac gccaaggtgg aagtggtctg cttccctagg aactgggcgg agatgagggg
    28021 aattttttcc gtgggcgatg tggtggtggt gacgggaaga gttcaggagc ggggggatac
    28081 agtgctcctg gcggaaaaaa tcatccccct ggaggtcgtg gagagagatc aggtccccca
    28141 ggtgcggctt cgggtggatg gccgggggat ttcatcggag gtggtgcgag aggtcttacg
    28201 ggagctgaag aatttccccg ggaagagccc cgtgcttttg gaagtgcagg acggctcgac
    28261 gatggccctc ttgcgattgc gcgacctacg agttcagaag ggagtgcttt cccccgagaa
    28321 gctgaaagac ctttcccagg ggcgggtgga gttggtgtgt tgaggggcag gaggagccgg
    28381 aggagccgga aggagaagga gagcgaagat gccgggatca cagggtcgcg aagcgctagg
    28441 ggcgggggga gagtctatcc tgggagattt catcgtggtg caggccttag aagacggggt
    28501 gacggtgatt ggcctgaccc gaggacacga gacccgattt acgcacacgg aaaagctgga
    28561 ccggggcgaa gtgtggattt ctcagttcac cgagcacact tctgccatga aaattcgggg
    28621 gaaggctcgg gtattgacgc gccatggggt ggccgagagc gggaggggtg accatgcgta
    28681 gaggaaagat cgtctgtacc ctgggtcctg cctgtagtga ctacgatacc cttcgggcca
    28741 tggcgtcggc ggggatggat gtggcgcggt ttaacttcag ccacgggtct tacgagaccc
    28801 atgccctgaa cttggagcag gtacgtcgcg tggagcagga gatggggagg cctttggcca
    28861 cgttgctgga cacgaagggc ccagaaattc gtacgggaac cttgaaggaa cgcgccccgg
    28921 tgacgttggt ggaagggaaa accctggtat tgaccacgcg ggaggaagaa ggagatgccg
    28981 cccgggtttc cgtgagctac gaccgccttt cccaagagct ctccccgggg atggacgttt
    29041 tcattgacga tggcaccctg cacctccggg tggagcacgt cgaaggcgaa gagatcacct
    29101 gtttggtgct ggtgggggga gagctagggg agcgtaaggg gatcaacgtc cccgacgcca
    29161 tcctgtccgt gcccgccctc acccccaagg atgtggaaga catccggtgg ggcgtggaac
    29221 accagatgga gtacatcgcc gtctcctttg ttcggacccg ggaggatgtg atggacgtcc
    29281 gtcgggtcct ggaagacttt ggggggactt tgggcatcat cgccaagatc gagaccaagc
    29341 tggcggtgca aaacctgaag gagatctccc aggtggtgga cggtatgatg gtggcccggg
    29401 gagacctggg ggtggaaatt cccaccgaag acgtcccctt ggtgcaaaag cgcatcattg
    29461 acctatgccg ctcccaaggg aagcccgtca tcgtggccac ccaaatgctg gactccatga
    29521 tacgaaatcc ccgtcccacc agggcggagg ccaacgacgt ggcgaacgct atcttggacg
    29581 gcgccgatgc ggtgatgctc tcgggggaga ccgccaaagg caaatacccc gtgttggccg
    29641 tggagaccat gggacgcatc gtcactcggg tggagagcga acgggaccgt tggcagaggg
    29701 gatgcaccgt gcccatcgcc gccaccacgg tccccgacgc cgtgagccat gcggcggtgc
    29761 gaatcgccga agagatgaag gcccgagtca tcatatccct caccgaaagc ggcagcaccg
    29821 ccagcatggt gagcaagttc cgcccggttt gccccgtcct cgcggggacc cccatcaaga
    29881 ggacctggcg gaagctcgcc ctggtgtggg ggctttatcc catccttcag gaccacgccg
    29941 ccacagcgga ggaagccctg gagggagcct tggccgcctc gctggagaga cacctcgtct
    30001 ccgaaggaga cctggtggtg gtcacggcgg gggtccccgt gggcatccca ggcaccacca
    30061 acatggtgca ggtccacacg gtggggcgca tcctgctcaa ggggctatcc ctcatccgga
    30121 aagaggcctc gggacgggtc tgtcgggctg ccacggcttc ggaggcccga gagaagatgc
    30181 gccccggaga cgtgctggtg gcgcctcaga cggaccaaag ctttcttccg gccatgcaag
    30241 gcgcgtcggc cctcgtcaca gaagaggggg ggctcacgag ccatgccgcc atcgtggccc
    30301 tggagctggg cataccttgc gtggtgagcg ccgaggatgc cctgcggatc ctagaagatg
    30361 ggatgttggt cacggtggat gggatccggg gggtcgtgtt cagcggaaag gtaagattac
    30421 ggtgatcctg gcccgcccct cgaggtcccg ggacaaagag gggcaatcct gatgggcctc
    30481 ctgggagtcc acgtctccgt ggccggaggg ctcgggcgcg cctttgaccg aggagaggcc
    30541 ttgggatgcg aggcgatcca gatcttcacc agaaaccagc tacagtggca tggggccgat
    30601 gtctccctgg gggagagcga agcctttttt cgccggtggc ggcagagctc cgtgaaggaa
    30661 gtggtctccc acgcctcgta tctcataaat ttagtggggg acccggagct ccggagcaag
    30721 agtttagacg ccttggtgag ggaaattcgc cgatgcgacg ccttgggcat tgacctttta
    30781 gtgctgcacc ccggaagctt tcggggagag agtgaagccg aggggttgac cctcctgggg
    30841 gatgcgttgt gccggaccct ggatgccacc gaggactcgc gggtgcgcct gctcctggag
    30901 accatggcgg gggcgggaaa caccctaggg gggaaactgg agcacttttc cgccgcgctg
    30961 gacatggtgg aagaccaccc ccgcatcggg gtatgcctgg acacgtgtca cctctgggcg
    31021 gcggggtacg attttcgcac ccccgccacc tacgaacacc tcatgggagc ggtgaataag
    31081 gccgtgggcg aagatcgagt ggggtgctgg catctcaacg acagcaagac cccccgagga
    31141 agccgccgcg accgccacgc ccacgtgggg gagggggaga tgggcgcggg ccccttcggg
    31201 ctggtgctcc aggatgatcg ctgggatgaa gttccctgca tcttagagac ccctaaagac
    31261 gggccggggg acgggggcaa tttggccgtt ctccgaaaga tggcggggaa atgatcctcg
    31321 gaggacctct tgagataccc ctcttctccc ccgccagggc cgaggcctta cgccctccct
    31381 taaggcgaaa cgcccctgcc ggtcgagctt gaggttgccc tgggtgaagg aaccccgtag
    31441 aatgggatcc gccctccggg ggccccgcca ccggagggtg tatccaaaag atcgagccta
    31501 gggatggaag gggggatcgt atgacgccca tggctgtgga catccgatgg caagatgtgc
    31561 ttgatatcgc catcattgcc ctgatcctct acaggctgtt gttgctcctg gtggacaccc
    31621 gggccatgca gctggtaaag ggcatgatgg tgctggcggt ggcggccctt ctggccaagg
    31681 tggcccatct ggaggccctc tcctgggtcg tggggaagct catgggagtg ttgctcttca
    31741 cgatccccgt catcttccaa ccggagctac ggcgcctatt ggaggagctg ggtcggggaa
    31801 atatctggcg ccgtcgcctg gttcaagaga gggcggagtc cttcgcggac gaggtgttgc
    31861 gggcgttgat ccaccttcag tcccggcgca tcggagctct gctggtttta cagcgagaga
    31921 cggggctacg ggacgtgtgg cgctccggcg tcaaattaga cgcggagatc acccaggaag
    31981 tcatcgtgtc catcttttgg cccgggaccc cattgcatga cggagcggcg gtttttgacc
    32041 gggagaagat tctggcggcc gcgtgctacc tccccctctc tgacacggtc aacctatccc
    32101 gggcctgtgg tacccgccac cgggccgccc tgggggtgtc cgaggtctcc gacgccttgg
    32161 cggtggtggt ctccgaggag cggggagaga tctctttggc caccaatggg cacctggccc
    32221 gagatctcaa agaagcccag ttgaggaaaa tgctgctggt ttacttttct aacgagcagg
    32281 aaacgatgcc ctttttcgag aaagtaaaac gtctcttttc gtcgcccaaa ccgcagggag
    32341 aagcgcatga gaaaaaacag tagatatgaa aatattttgg attcccgctt tttcctccag
    32401 atcatttccg tggggttggc gatgttgctg tggttctacg tggcggggga tcggacatcg
    32461 gaaacggtta aaagctttcg cctgcctctg gagtatcagg gattggggcc ggggctatcc
    32521 cttaacaacc cttcgtcctt cgtcgacgtg caggtgacgg gaaaagtggc ccccctgcgc
    32581 ggcctgaccc aagaaaactt gagctgtttc gtggccataa gggatctggg gccgggaaaa
    32641 tatacgttac ccgtgacgcc ggtcttgccc tccggtattt ccctggtgac cgtcagcccg
    32701 tcgtcggtgc gggtggaaat ctcccggaac gtggagaaaa ccatgcccat caccttgcgc
    32761 gtgcagggag actttcccga ggggtacgcc ctggtggcgg ccagcctgga tccccccgag
    32821 gtgttactga aggggcccga cacttcgctg gggcgcatct cggaagtctg ggcagcccct
    32881 cgggcgcagg acttggtgga cgacggagac ggagtgatgt ctctgccgct cagcgcccaa
    32941 ttctccggcc tccgagacga tcgggtgtgg atggaaccga aggacgtgag acttcggttc
    33001 gaactttccc gagagctccc ccagaagcag gtgcccctgc gagcccacgt ttttggcgac
    33061 gtggacccca actacacctt ggccatggtg acggtgcgtc cgcccacagc cacggtctgg
    33121 ggcccctcct ccgtgctggc cggagtgtct tttcttgaca tagaaagcgt ggacgtgtcg
    33181 gggctgagag gcaatgccac cttctcggcc aacatcctgt ctccgggaga agggctcacg
    33241 gtctatccct ttcccacggt acaggtgcag gcgacggtgc gcccggtgct gatctcccgg
    33301 accttttcgt cggtccccgt cgatgtggaa ggaaggagca tctacccccg atggaaggtg
    33361 gaccccaaca aggtgaaagt gaccctccgg ggcctcgtgg gggtcttgag cggtgacgga
    33421 gcccctcggg cctacgtgga agtgacaaac ctcgtatccc gccgggtaac ggtgccggtg
    33481 agactccggg gacgcagtgc cgatgtggaa gtggtggaga tctctcctcc catggtaacg
    33541 gtttacgctt tggtggaatg aaagaaggga aatctgaaga agcctggagg aattgtcatg
    33601 ttgacgggat cgggtagggt tcgttgtctt tttgggaccg atggcgtacg ggacgtggcc
    33661 aatcgcggga tgctgacgcc ggaaatggcg cttcatctgg cccgggcctt cgtgtggtgg
    33721 ctcgtgcgcc gcggggtgtc ccggcctcgg gtggtggtgg ggcgagatac ccgtcgctcc
    33781 ggagacatgt tggaggcggc cacgatggcg gggctcatgt ccgccggagc cgacgccctc
    33841 acggtggggg tgttgcccac tccgggagtg agctttgcca ttggcagcgt gggggcctcg
    33901 gggggagtgg tgatcagcgc ctcccacaac ccggcggaat acaatggcat taagtttttc
    33961 gactcccaag gatgcaagct ctctgacgaa gatgaagcct ccatagaaga atacatggac
    34021 gaccatctcc tggacgactg gcgccctacc ggagcctcga cgggggtggt gggagacggg
    34081 gctttcctga gggagcgcta ccagcattgg ttggaaagcc tggcggggga gcatacttgg
    34141 cccggccgcc tggtggtgga ttgcgcccac ggagcggccg taccagtggc gggccccctt
    34201 ttcgccctcc tgggggcgga tacggtggtg acaggggatc gccccgacgg gctcaacatc
    34261 aacgaagacg tgggagtcat gcacatgtcc cacctggtgg ggtgcgtcaa ggagcaccgg
    34321 ggacaactag gggtggcctt cgacggcgat gcggaccgcg tgctcttcgt ggatcgccag
    34381 ggtcgcaccc tggatggtga cgtgctcctc tgggtcctgg ctcgctggct gcgccgtcgc
    34441 ggcaccctgg ggtcgggggt ggtggccacg gtgatgagca acatggccct tgaggagcac
    34501 ctgggagaag aacacatccc cgtgttccgg tgccccgtgg gagatcgcta cgtccttgag
    34561 accatgaaac gagaagaaag ccagttggga ggggaacaat cggggcacat catcatccga
    34621 gagttcgtct ccacggggga tgggctctgt tcggccctgc tcttcctgag ggcctgccgg
    34681 gagttgggag aggacgtgga caccctggtg gaccgcttcg cccggtaccc tcaaattttg
    34741 cgcaacctcc acgtgaagga caagcgtcgc atcctggagg atgataattg gcaggtcttc
    34801 ctgaaccaag ccgaggcccg gatgcagggg gtgggtcgta ttctggtgcg cccctccggc
    34861 accgaacccc tggtgcgcat cctggtggaa gccaaagatc ccacgctggt gcaggccgtg
    34921 agtgacgatc tagaggcccg aatctgcgct ctgaacgcag aaaattaaaa aattttaaag
    34981 gagatagaga tggctttttc ccgtaaggtc accaagtgtc tttttcccgt ggcgggcctc
    35041 ggaacccggt ttttgcccgc aacgaaggaa accccaaagg aaatgctccc cctcatcgac
    35101 cgccccatca tccactacgg tgtggaagag gcggtatctt cggggtgtgg cgacgtgatc
    35161 ttcgtcacgg ggcggggaaa gcgctccatc gaggactatt tcgaccgctc ttgggagctg
    35221 gaggacttgt tggagcgtcg cggtaaggac gatttgtgcc ggttggtgcg cgacattacc
    35281 cagatggcgc gattttccta cgtccgtcaa tccgagccct tggggctggg gcacgccgtg
    35341 ctctgcggcg aactcatgtg ccaagatggc cccttcggcg tcatcctccc cgacgacgtc
    35401 atgcggggag atcccccggt gatgaagcag ctgatcgatg ttcatgagtg ccacgggggg
    35461 tcggtgatag ccctggagga agtgtccccg gaagacactt cccgctacgg cattgtggcc
    35521 gccgacgatg tgggaaacgg ggtgttccgc ctcaaggata tggtggaaaa gcccgtggcg
    35581 ggtcgggctc cgagccgcta cgccattatg gggcgatacg tcttgtcccc cacgatcttc
    35641 gaagagctcc gctccttgaa ccccggagcg gggggggagt accagctcac cgacgccctg
    35701 cgatccctcc tcctccggga acccatctgg gggtacctgt accggggaaa gcgcctggat
    35761 tgcggcacta agcagggatg gctccgagcc accatcacca tggcgtggga ggatccggaa
    35821 ctcaagggaa ttcttcaaga ggtcctgggg gatgaagcgt aaggccgtcg cctttggcga
    35881 tagagccctc gacggtcccc gataattttt tttcgatgga gggcttcttt tccgcccggg
    35941 gccctaggag ggggtggtcc atgtcaaaaa aactttagcg ttttggttaa gcgccagaac
    36001 cgccagagtc cttcgatccc gcccgaaaag acgggacaga agaggcggtg gacgaggtct
    36061 ggggagtatc gaaccttcgg cggatgcccc agggagctgg aagggctcct aggcagggac
    36121 aaactccggc gagtgatccc ggaacaaagc ccgtgccacc ttctgcggac ggctagagag
    36181 gagaaagttt ttatgtgtgg tatcgtggga tacgtcggac ctcgcaacgc ctgtgacgtg
    36241 ttgttggagg ggctgcggcg gctggagtac cgagggtacg actctgcggg gctggcagta
    36301 tggaccgccg aaggattacg caccatcaag gaagtgggca aggtggcgga gctggccgac
    36361 gcggtgcgcg cagcgggatt cgacggattt cggggcattg gccatacccg ctgggctacc
    36421 cacggggggg tgacccaggc caacgcccac ccccatgcgg atgaccgcca tcgtttcgcc
    36481 ttggtccata atggcatcgt ggaaaactac agcgaactcc gggaagaact ggaaaaacag
    36541 ggcgccattt tttcctccga gaccgacagc gaagtggtgg tgcagcttct ggctcgcatc
    36601 atgggagaga ttgcttctat gccggaggcc ctaagggaac tccagggacg cctccaggga
    36661 tcctttgccc tggccatcat ggacggtgag gatccggacc attttttctg tgtgcggcgg
    36721 ggatcgccgc tggtgctggg gctggggaaa ggggaagcct tttgtgcctc ggacgtttcc
    36781 gcccttctgc cctatacccg ggaggtcatc tacctggaag acggagatat ggcccgtctg
    36841 ggcccctgcg gggcgacgat gtggaacgct acgggggaag agatccatcg ccccggatcc
    36901 atggtggatt gggacgtgac catggcggaa aaagacggct tccctcacta catgctcaaa
    36961 gaaatacacg aacagggagg cgtgcttcgg gcggccttca aggggcggat cttccccggg
    37021 ggggtagatc tttcggggga gctggattgg gatgacgccc tcatacgatc ctggaaacga
    37081 attcacgtgg tggcttgcgg tacggcccat tacgcctctc tggtgggaca aagggtcctg
    37141 gagcgctgga ccggcctgga ggtgctcgcc gacatcgcct cggagtaccg ataccgtccc
    37201 ttgcgggccg acgccgacac gctggctatc ttcgtatcgc agtccggaga gacggccgac
    37261 acactggcgg ctttgcggaa ggcccggagt gaaggggctc gatgcctggc ggtgaccaac
    37321 gtgcgagggt cgactctagc ccgagaggtg cacgacaccc tgctccttcg ggcggggccc
    37381 gagataggag tggcggccac caaaaccttc atggggcaga tgggtaccct ctatgccctg
    37441 gcgctctacc ttgggctgaa gcggggaggg gtgtcttccg ccgaagctaa aagactttcc
    37501 gaggggcttt tacagctgcc ctaccgagtg gaggcgttac tcacccggct tgacgcctcg
    37561 gtgcgggcgc tcgccgctcg ctacgcgggg gcgcggagtt tcctgtttct cggccggggt
    37621 cactcctacc ccgtggcgct ggagggggcg ctgaagctca aggagatctc ctacgtgcat
    37681 gcggaggcct acgccgccgg ggaaatgaag cacggcccca tcgccctcct ggagcccgag
    37741 ctccccgtct ttgtggtggc cccccgggat agcttgtacg agaaaaccat atctaacatt
    37801 caggagaccc gggcccggaa ggcccccgtg ttggcggtgg ggaccgaagg ggatacgctc
    37861 ctatcgtcgc acgtggaaca ggttatcgcc gtgccctggg cggaggagga attgacgccc
    37921 tttttgaccg tcatccccct gcaacttttc gcctatcacg tggcgctttc gcggggatgc
    37981 cccatcgacc agccccgaaa tctggccaaa agcgtgacgg tggagtaagg gtaaaggagt
    38041 aaaagaggtc agaccttccc cgaaggtaag gtctgacctc ttgggacctc tcacggccaa
    38101 ggccctcccc aagggatctt tcggggggcc ttagcgcctc tcctggcgcc gacgccaggg
    38161 tgcgacttgg tctcctttgc agagccccgt tgttctcaag gcggaggctt ttccccccca
    38221 aggggtcggc agttcggggg gatatcagtc ttttttttga tgcagcatgt taagcagttt
    38281 ttgcctgatg acctcgggct tgaacggttt gacgataaaa tcgacggccc cgtggcgcag
    38341 ggcctggatg aggcgcggct tgtctccata cccggatacc acgatgacgg gaacgtgctc
    38401 ccactgggtc ttttcccgaa ggttttgcat gaatccaaag ccatccatgt agggcatgag
    38461 aaggtcggtg atgatcacgt gcgggttggc cgaaggaagc tttcttaggg cgtcctggcc
    38521 attttccgcc tccaccacca cacaccccat cttggacaac agacgactga gtcctacccg
    38581 caccacggcg gcgtcgtcca ccacgaggac ccgaaagtcc ggaggaaagg gggtatcttc
    38641 agagaccgtc gttttttccg gtacgggggc gggagcaggg ctcggggcgg aaacttcttc
    38701 cacgggaaag cccagggctc gacggatttg attggggtcc gggggaaggg ccagcagatc
    38761 gtcgcacccc gccacccggg cgcgcatccg ggcgcggatg tcgctccact ccggcgacaa
    38821 ggcaaagaac tgggtactag aaccccttgc gtggagccgg atcgcctgga tctgcgaggc
    38881 ctctccctcc tgggtctgcc cccagcctaa aaaaaggacc tccagagggg cgtccaggtg
    38941 atccacgccc atgaccacat cccccagatc atcccaggtc gtgcacgcca accccaggga
    39001 gcgcaggagc ccttccaggg ccatgcgcac cttggggtcc ttgatggcaa ggcccacggt
    39061 ggcgtcgggg gagatcatgg gtaaccggag ctcctaagaa cttttggcat agcaacctcc
    39121 tcggaaaatt atccccgaaa ggccccagag aaacagccga aaagtcccgg gagcaccgaa
    39181 gggaactagg gcttagattt cccactgacg gggtcggagg atatcaaaaa ggacgttatg
    39241 ggttcgggaa aaaccatacg gcccagacgt tacacagggc atggtacaga agcggagccg
    39301 acacggtgtc gtgccgttct cgcaaatacc ccatgataag ccccgggaaa aatgtggcga
    39361 ggaagagggg acgactctgg aaaatcatgt gcgatgcggc aaaaagagcc gacaccaaca
    39421 ccacccgtcc catcgttccc acccagggtc tgagggaaga ctggacccaa ccccgaaaga
    39481 agatctcttc cacgatggcg gcggtggtgc ccgccatcac aagttgtccc acccctcccc
    39541 acggtatcgt gcgaggaatg ccccccgtgg gccaaaacca cgccaccccc gtgaggggga
    39601 tcagggtgaa cccgcaccag aagagcaccg accgcaccag ggcaggatcc cagcgccaag
    39661 tcaggccata cctctggggg gattctcggc ggaggacaca ccagagaaag gggccgtaca
    39721 acatgaaggc cgctagggcg aatcgcgcca tggttccgct cccattttag aaaaagtcgt
    39781 gccccgcttc tgccccgttt acttgacgac accccttcga agaagctcga ggaactagga
    39841 agggacgtcc ctttagcctt ccgagggaca cgcctatttt cgggggatcg tgctgcttgc
    39901 caggtctccc gccccgtttc cacaaaacaa ccaatccccc ccatcgacga tggggattcg
    39961 ctggcgaagg gctttctcgg cggggcgccg aaggggaccg aagcctactt tttgcccgac
    40021 cctgaaatgg cccacaaaaa agacccccgt cccatcgtcg gggtgggagc ccagggctcc
    40081 gtccaataat acaccatgga aggtttttgt ggggaaaaaa acgacgggcc agaacctttt
    40141 tggttctgga ccgtctggag atgcggcgcc gaggaaaatc aggcgtttcc tgctcgttcc
    40201 tgggcggagg ccaaacagga ggggcagact ccgtaaaaaa agacctggac ctcgtccacc
    40261 cgatgaggcc ggtctccccc aaaagagggc aggccctttt ccggcagagg gatatcttct
    40321 acgccctggc attgggaaca cttgaagtgt ccgtggaagg cggcgttggg atcaaagatc
    40381 acatgacggt cgtcgatggt gaggatggat atgagcccgg cccgggcgag gagctccgcc
    40441 gtgccgtaga tggtggcaaa ggagatggtg gggaaggtcg ctcgcaactc cttgtagagc
    40501 atctccgccg acggatgatc tttgcgcccc tccagtcgtt tcagtatggc gatgcgctga
    40561 gcggtgatct tggctccctg agcctttagc gtttgcagcc cttcttcaag agtccacatg
    40621 agagatcctc cttaagtcgt cccgggcccc ggggagaggg gcatggctat ttgatatcct
    40681 tagtagctta aaagcattat aagtcccctc cgcccctttg gcaagagctc caggatggcg
    40741 aatcccatcc gggggctagc gccgtgctta tcgttaaaaa acaaacatct ctttgttttg
    40801 ttgggaagga atcgaaaagg gatccggggg aaagaagggc cgcgcccggc accaagagcg
    40861 gtttcggacg cgaccacagg ctgtcgacgg aaggaggagc gaccaatctt gacaatcaca
    40921 aaaagtcgcg agataatcca aaactgcggg cgtattgggg tgtagccaag aggcaaggca
    40981 ggggactttg gatcctccat ccgctggttc gaatccagcc accccagcca attcagatct
    41041 gggaaagggg ctttacccgg ggcccctttt tgtgtggtcg tggtgcggtt tcctcgggag
    41101 ctttctttcg cgccgcgagg ccgatttatc ggcatgttaa cgaaggggtg agacccatgg
    41161 gtaacgcggt aaggcgaggt gcccttgtct tggcggccgg aaagggcacc cgtatgaaga
    41221 gcgcgactcc caaagtcatg cactcgatcc tggaagagcc gatgatttcc taccccctga
    41281 gggcgaccct gggggcgggc tacgatcgcg tggcggtggt ggtgggcttt gggggggaag
    41341 aagtccaggc ctacctccaa gctcattggc cccaggtgga ggtcctctgg cagcgcgagc
    41401 agctcggtac ggggcatgcg gtgatggagg tccggggttg gtggcagtcc ctggacgagc
    41461 tggttatttt gtacggagac atgccctgcc tcacccccga ggccctaagg gccctggata
    41521 aatcgacggc agagggggct ttcctgtcct tccgggcggg agatccccgg ggatacgggc
    41581 gggtggtgcg gggggcggga gcggttcgta tcgtggagga ccgagatgcc actccggaag
    41641 agcagcgcct ggacgaggtc aatagcggca tctaccgttt ttctgttccc ctcatggcga
    41701 aagccctgga aacccttagc ccggccaatc aacagggaga atactacctc accgacgtgg
    41761 tgcacgccct gggtgctacg gggcggggtg tggaccccgt gctctggccc gaggccgagg
    41821 atctagaagg agtcaacgac cctcgccagc tagcagcggc cacggccgtc cttcgcgatc
    41881 gtatcctggg ggaggccatg gggaacggcg tgcggtgtct tgacccttcc tccgtatggg
    41941 tggggcccga ggtggtgctg gaagaggacg tctttttggc gccccaagtt cagatcttcg
    42001 gcaaatcccg catcggcgca ggatctacgg tgggctcggg aagtatccta aggaacgccg
    42061 aggtggggcg ctcttgtcgc ttggtgtcct acgtgtgcct ccaggatagc gtggtgcgcg
    42121 atggagtctc cctggggccc ttcgtcgtca ttcgagacgg gagctgcttc gaagcagggt
    42181 ccctcgcggg aaagttcgtg gaggtgaaaa aaagcatcgt ggggaggggg agcaaggtcc
    42241 cccacctctc ctacctcggc gacgccactg tgggagaggg gaccaacatc ggggcgggga
    42301 ccatcacgtg taattacgat ggagtcagga aaaatcccac catcatcggt gatcgttgct
    42361 ttgtcggcag tgataccatg ctggtggctc cggtgcgcct tgatgatgac gtgaccaccg
    42421 gcgccggatc ggtcatcacc caagacgtgc ctccgggggc cctggcggtc ggtcgggccc
    42481 ggcagcgtaa cgtggaaggg tggaaggccc gacgccgggc gaaggaggag gggtaatggt
    42541 tacgcggttg cgggagatca agatcttttc cggttcggct aatccggcct ttgcccagga
    42601 aattgcggat cacttgcgag tttccgtggc gtcttccaag cgctttcgct tcgccgatgg
    42661 agaaatcggc atttccatcg aagagagtgt gcgaggggcc gatgtttacg tggtgcagtc
    42721 cacctgtcgg cctgtaaacg agaatttgat ggagcttttg atcatggtgg acgccctccg
    42781 gagggcctcg gcctatcgga tcaacgtggt caccccgtac tttggctacg cccggcagga
    42841 ccgaaagact cgggggagag agcccatcac ggcgaagttg gtggcaaacc tcctgaccca
    42901 tgcgggggtg catcgggtca tcgcggcgga cctgcacgcg gggcagatcc agggcttttt
    42961 tgacattccc gtggaccatc tcaccggcat gcatcttttg gcagcccatt tccgggagca
    43021 gctcaaaggg agtatcgacc agggggaggt ggtggtggtc tcccccgatg tcgggggagt
    43081 ggtgagggcc cgaaagtttg ccgggcagct caatgccgat cttgccatcg tcgacaagcg
    43141 ccgatcttac gagaagacaa actcttgcca ggtgatggag atcatcggcg acgtccaagg
    43201 aaaggatgtc atcctggtgg atgatatcat cgacaccgcc ggaaccatgg tgaacgccgc
    43261 cgccgctctg gtagaaagag gcgctcggag cgtctacgcc tgtgccaccc acggggtgct
    43321 atcggaaccg gccttggagc gcctgcgagc ctcgtgcatc caaaaaatgg tcctggccaa
    43381 taccattccg ttgcccccgg acgagaaacc ggataaaatc gtacagctgt cgatcgcccc
    43441 tctttttgcc gaagccatcg accgcattca ctcggatcac tccgtcagca tccttttcgg
    43501 tgcttagggt cacggcttcc ttcgcccgag gggcggggag ttcaattttg gaagggggaa
    43561 atgtttcatg tctggattca tccctatcac gctggaatcc cgagagggga tcggcaagga
    43621 aaaaagcggc cagctgcgtg cggcagggta cctgccagcg gtgttctatg gccccgacta
    43681 caaagacggt atgccggtgc gctttcaagc taagagcctt ttgccccacc tgcgtaagtc
    43741 tcactgggag acgttgcgcc tagacgtgac gctccccgac ggtaaacagg agatggctct
    43801 gcttcgggat ctgcagcgag acagcatctc cgacgaggtg ttgcacctgg atctctacca
    43861 attggtgcgg gaccgcaagg tgcgcgtggc ggtgcccgtg cgcgtgatta accgggaagg
    43921 atgccccggg atcaaggcgg ggggagtctt tgagctgttg caccgcgtgg tggagatgga
    43981 agtccttccc cgggagatcc cggacgacat cgtcatcgac gccgcctccc tggataaggg
    44041 aaagggcttt cgcgtgaggg agtgcgccct gcccgaaagc gccgaagtcc tgttgggtcc
    44101 cgaagacttt gtggccctca tcagcgagcc ccgggccgaa gaagaggaag aggcggaggc
    44161 cacagcgggc tccatggacg tggaagtggt ccagaagggc aaaaaagaca aggacggggc
    44221 ggcctaaggg gtccgctgtt gtcctagcgg gtttttttct ccggggaagg gcggagcgtt
    44281 gcgcctgatc gtggggctgg ggaacccggg cccagagtac gcctggtccc gacacaacgc
    44341 gggatggatg gtggcggacg ctgtggcgga gaagttcggc atggggactc cagcggtcaa
    44401 attttcctcg ctgttttggc agggacgcct tcccgacggg gagaaggtgc tgtgtgccaa
    44461 acccgtgacg ttcatgaacc taagcggcgt ggcggtgcga gaaatggtcg cctaccaccg
    44521 cttggcgccg gagaggattc tggtgttgta cgacgacgtg gcgttgccct tcggccagct
    44581 caggctccgg ggaaaggggt cgtcgggggg gcataacggc atggcttctg tccttggcgc
    44641 cttagggact ttggaggtcc ctcgtctgag ggtgggggtg ggccccctcc cggcgggatg
    44701 ggatttggtg tctttcgtgc tgggggcctt tcctcgggac caacgggagg cgatgccggg
    44761 cgtggtggac cggggggtgc gcgcggtgga gttgtggatc gccgagggcc tcgagggggc
    44821 catgggccgg gtcaacgggg cgggaaagat ccagggctaa gatcccgtcc gtggggaggt
    44881 ctaccccggc cctttttaga aagggacgaa gggccggagc ggggaaggaa gttttgtctg
    44941 cgtcttcgta atggaggaga cttcggagga tcggtatgac ggcgtggagg gatgggggag
    45001 gacagttctg ggacctgccc tccgaggcgt ggtatggcgg ggcgggcgtt caccttgcga
    45061 cggcgggggg ggcccgcccc tgggcttgtg cccatccgaa ggggcccatg gtggtggtgc
    45121 tggcggatct gaggcagatg gagacctttg ccgaggactg gaaggccctg gatccggaaa
    45181 aatcaggagg aacgctgtcg gtccttcgag agcttcctct gggtccggag gccgcaggga
    45241 accgggctta ctggatgcag cggggagaga cgctccgtcg ttggcgccac cgaggagggg
    45301 tactggtggc cactccgggg gggctcctgg gcccctacga ggaaggcacg gagacccttt
    45361 ctctgcggga gggggaggtc gtggggtgga atcgcctcct ggcctggctg acggagggag
    45421 gctatggtcg aagcgacctg gtctgggccc cgggacagtg ggtttcccgg gggggcatcg
    45481 tggacgtctt cgatccggcg gagccccttc ccctgaggat tgagttttgc gatgatgaaa
    45541 tttccagtat tcgctacttt tccccggaga accagcgaag cgtaggttca ttggggacgg
    45601 tggagctccg gcgcctttct gattcccgga gtcttcgaag ggggattgcg gcggagcgaa
    45661 tccccgaaga cgtacctgtg gtttttttcg agccccgaga cgtggaactc cagggggatc
    45721 tctacgaaga gctctggaac ggcttgcggg aacaggagga cctccccgaa attccccctt
    45781 ggcaggaagt ggccctatcc ctcttccgcc acccccgcct ccgcgtttcc cgtgcgctgg
    45841 ccccgggaga tcatcggatg gcgatccagg agcttccccc ctttcgggga aagatcaaag
    45901 atgccgaggg ctttgtggcg gcttggcgcg accggggatt ttctctcctt ttggtgtccc
    45961 gcaccgaagc cgtacggctc tggggagagc aacgaggttt tcacgtgcga tcgggatccc
    46021 tatcgggggg ctttttagat ctggactccc gatgcggggt cttttcggac gcggaattgg
    46081 tgggggtatc cctttcttct ccctcctcgg ggtggtatgg ggtccctcgg gaatgggcgg
    46141 atcgattggc tccccaatcc tgggtggtgc acgaagatta cggggtttct cgatttttag
    46201 gaacccaagg ggtggaaacc ccccagggag agcaggacta cctcgtgctg gagttcgccc
    46261 agggcaaacg tctgttggta ccggtggccc aatttggcaa ggtgactcct ttttccgctt
    46321 tgccggaaga agagcccact ccggacgccc tttcgggttc ccggtggcgc aaggccgtac
    46381 aacgagcccg agagcgagcc gcagaagccg ccaagaagct ggcgaacctc tatgccctcc
    46441 gggagacgac ggcgggcatg gccttccccg ccgacggcga aatgttgcgc atggtggaac
    46501 actcctttcc ctacagagag acggaggatc agctccgggc catccgggac gtaaagaaag
    46561 acatggagca ccactacccc atggaccgat tggtggtggg agatgtgggg ttcgggaaga
    46621 ccgaggtggc ccttcgggcg gtgctcaagg ccgtggaagg gggcagacag gcggcgttcc
    46681 tcgtgcccac cacgatcttg gctcaacaac atcatggcac cttcgaaggg cgcctggcgg
    46741 ggctcccctt gcgggtggag tcgatctccc gcttcgtctc ccccgcccgt cagcgacgca
    46801 tcctcgccga taccgccgaa ggcaaggtgg atctgttgat cggcacccat cgtcttctcc
    46861 agaaagacgt gtcctttgcc cggctggggc tcttggtggt ggatgaagaa caccgtttcg
    46921 gcgtgctctc caaagaagaa atcaaggggc gatttcccgg ggtggacgtc ctcaccctca
    46981 ccctacgccc attcctcgga cccttcagct ggccctggga gggatgcgcc ccctatccct
    47041 catctccact ccccccgagc accgtttccc cgtcctttcg gtggtctctc cctggagaga
    47101 cgagctggtg cgcaaggccc tccagcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    47161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    47221 nnnnnngtcc tttcggtggt ctctccctgg agagacgagc tggtgcgcaa ggccctccag
    47281 cgagagttgg cccggggggg gcaggccttt tttgtgcaca atcgcatccc ccagctggaa
    47341 tctcgagagc gatggctacg ggaggtgctg ccgggatcgc gcgttgccgt ggcccacgga
    47401 tccctaccct ccgaagggct ggaacgggtc atggaagatt tttttctggg gaagtccgac
    47461 attctcctgt gcaccaccat cgtggaaagc ggcctcgacg tcccccaggc taacaccctc
    47521 atcgtggacg atgcccagca cctggggctg gcccaaatgt accagcttcg ggggaggatc
    47581 gggcgccgca gtgcccaggc ctttgcctat tttctctacc cggaggggga aaccctatcc
    47641 cgagaggccc aagaaaggtt agaggccatc ggagagatgg gagagttcgg gtcgggatat
    47701 cgattggcca tgcgggacct ggagattcgg ggagggggag atttcctggg attggcccaa
    47761 catggccacg gacctagggt gggtttttcc ctgtactgtt cgctgcttga aaaagaactg
    47821 acggcagtga tgggtggtaa aatctcgaag accgcgatcg ccatcaactt tcccctgggg
    47881 ataccccggg gatatgtcgg ggaagaaagc gtgcgcatcg cgctttatcg aaggatcctc
    47941 cgcctggatt cttgggaaga ggtgcaatcc attgaaagag agctacggga ccgctttggc
    48001 cctatccccg aaagcgtggg gtcgttgttg gatggggctc gaattcgcgc cctggggagc
    48061 catttcgggg tagaatatgt cgacggccga gcggatcgta cggaggtcgg gggggaaact
    48121 ttgggccgtt cccccctggg ccgcgccctc ccgggatgga tgccggggaa aaggggtctc
    48181 gtgggccccg gaggggcccg aggactacga gatttggccg aagccatgaa gatcctttcg
    48241 gaagcctcgg aaggaacgaa atcttgagag ggggctctgc gatgacggga gaagaagaaa
    48301 gcgccccccg gggcgccgtc ttggaaagtc tacgaaaaaa tgacgaagaa ggggctcccg
    48361 aggaagggcc tcgggactcg gggtcgtctt tttccaggct gatggatgtc atggcgcgcc
    48421 ttcgtgcccc cggggggtgt ccgtgggatc gggagcagac ctgggaaagc ctgaagccct
    48481 atgtggtgga agaagcctac gaattactag acgccataga ctcctccgac gatgagggca
    48541 tccgggagga gagcggagac cttctcctcc agacggtctt catcgcccag attgcccggg
    48601 aggaggggcg cttcgacatg ggagacgtgc tggaggggtt ggtcgaaaag ctcgttcggc
    48661 gccatcctca tgttttcagc gacgtctcgg cggagacgag cgacgcggtg ttgcgccact
    48721 gggaaaggat aaaatctgta gagcgccgct gccggggaaa ggatctgtcc atgctcggtg
    48781 gcatcccccg gagccttccg gcgttacttc gtgcctatca gatgcagcat cgggccgcca
    48841 aggtggggtt tgactggccc cagggagacc ctcagccagt actgaataag gtccaggaag
    48901 agatcgagga gcttcgcgaa gccctgatca ggggcgacaa ggatcaagtg gccgaagaga
    48961 tgggggatct gcttttcgcc atggtaaact ttgggaggca cctggggatc gacgccgagt
    49021 ccgccctgca ggccgcctgt cacaagttcg cccggcgctt cggctccgtg gaggaagcgg
    49081 tgcggctcca ggatcgcccc tgggagagct tttctctgga agagctcgag gagttctggg
    49141 gcaacgccaa ggcgagcgaa cgggggccgg aggcctccga tcccccgagg tttgggcacg
    49201 aagcctaatc atattgctag tttttaagaa aacatgagga ggaatggtcg tggataagga
    49261 acgcaagggg cagagtgggt cgaaaaacac cggagcttcc gcagagaccg tcgtcgcggg
    49321 ggggattgag gaggccaaga aaaacgatca tcccgccaag gaggtcctgg aggggcgccg
    49381 ggtggggatc atggagaccg ccctgcggga tgcgcaccag tccatcatgg ccacccggct
    49441 tcgcaccgaa gacatgctcc ccgtggcgga ggtcatggat gaggtggggt acgcctccct
    49501 ggaagtctgg ggaggggcga ccttcgacac gtgtatgcgc ttcctggacg aagacccctg
    49561 ggaacggctg aggctcctgc gaaagcgctt caagaagacc aagctccaga tgctcctgcg
    49621 ggggcaaaac ctggtggggt atcggcacta tggcgacgac gtggtgcggg agttcgtgaa
    49681 gcgcgccgtg gggaacggca tcgacatcat ccgggttttt gacgccttga acgatctgcg
    49741 caacatggaa gtggcggccg atcaggtcaa gaaagaggga gcccgccttc agatggccct
    49801 ttcctacacc atctccccgg cgcataccct ggaggccttt gccgacttga gcgtccagat
    49861 gaaggacatg ggggcggact ccctctgcgt caaggatatg gcgggcctcc tgagcccggt
    49921 ggcggcccgg agtctagtcc agtccatgaa ggatcgcacc ggacttccgg tacaggtgca
    49981 cagccactat accagcggca tggcggccat gtcctacctg gcggccattg aagccggggc
    50041 ggatgtcgtg gattgtgcca tatctccctt tgccatgggg accagccagc ccgccacgga
    50101 gaccctggtg gcggccctgg cgggagggcg ctttgatacg gggatttacc tggagaagtt
    50161 ggagcccgtc tcccggtatt tcaaggaaat ggcggaaaag tatcacaaga tgatgatggg
    50221 cctcgaaggc gtggacatca atatcttgct ctatcagatt cccgggggca tgtactccaa
    50281 cttggtgagc cagcttaagg agcaaaacgc cctggaaaaa ttggaggcgg tgctgcaaga
    50341 ggtgccccgg gtacgccgag agatgggata tccccctctg gtaactccca cgagccagat
    50401 cgtggggagt caggcggcgg ctaatgtctt ggtgggagag cggtggaagg tggtccccaa
    50461 ggaggttcgc atgtactttt tgggttacta cggcaaatct cccgccccgg tggatccaga
    50521 ggtggcccgc aaggccacgg ggggcgaaga gcccatcacc tgccgtccgg gggaaaagct
    50581 gggcgacgag ctccccggcg cccgagcggc ggcgggacct tacctgcttc agcccgagga
    50641 ccttttgagc tatgtcctct tcccctcggt ggctaaagac tttctggtga aaaagtttgc
    50701 ccgggagacc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    50761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gaggaagaga
    50821 ttcgccccgc cctccagagc cacggggggg atatagagtt tttaggggcc gacctggagg
    50881 aaggatcggt gaaggtgagg cttacagggg cttgcggagg ctgccccttt gcccaagaga
    50941 ccctacgcac gcaggtggaa gaagtcctca agggccaaat ccccgagatt cgccgcgtgg
    51001 tccggggata gctccatggc ggaaccttcg cccgcagaag gggcccccgt cgccggagcg
    51061 cccctagggg gcgagcctcg gaatcgaaag ggaccgcccg gatccccggg ggtggcttgg
    51121 tccttgcccc tccgaggaga agagctggtc gccctgttag ggaaaaacga tgaacacctc
    51181 ccccttctag aggaacgtta ccccgtgcgc cttttcgtga gagacgggga gcttcgggtg
    51241 gaggggcccg acgcttcggc ggtggagacc gtcgggaccc tcctttccga gctggccaag
    51301 gtggttcgtc aggggcatac cctttcggcg gccgaggtcc gttatgccat ggacgccctc
    51361 gcccaagggg agctcccgga catggcatca cttcacggag aagtaatttg ttttaccagt
    51421 cggggcaagc ccgtgcgccc ccgcaccttg ggacagcgac gctatattga tgcggtgcgc
    51481 cgaaactctg tggttttttg tatcggcccc gcagggacgg gcaagaccta tctggcggtg
    51541 gccctcgccg tggcggccct caaagcccag agcgtgagtc gggtgatctt ggtccggcca
    51601 gcggtggagg cgggggagag cctggggtac ctccccggag acctgaaaga aaagatcgaa
    51661 ccgtacctac ggccgctcta cgacgccttt tacgacctcc tgtctccgga gcgcttcctt
    51721 cggtacgcag ccaaaaacgt catcgagatc gcccccctgg cctatatgcg tggaaggact
    51781 ctcaacgaca gctttatcat tctggacgag gcccaaaaca ccactccgga gcagatgaag
    51841 atgtttctta cccggatggg cttcgggtcc aaggtggtgg tgacggggga catcacccag
    51901 gtggatctcc cggcggggaa gatgtcggga ttgcgcgtca tttcctccgt gctgcagggg
    51961 atcgagggga ttgagtttac ctccctggac aatcgtgacg tggtgcggca cgaaatcgtg
    52021 cagaggatcg tgttggccta tgaagatttc gagagtcggt cgtagcgtcg atcggtggtg
    52081 gtttttcgcc ctatctcggt ggaaagaaac actgcccctc tggtgtatgc ggggggggct
    52141 ggtactggtg gccctggcta ttttcctcgg agggtggtat ctggaccggg acctagaggg
    52201 attttccgtg ggccagcccg ccccgcggac ctttctggcg gtcttccgca tgcgctttga
    52261 ggacgtggaa acgacggaaa aactcaagct tcgcatcgcc gatcgggtgg tgggcgtggt
    52321 ggtccaggat ccatcccggg tggaagacat cctggtaaag cttgatcagc tccaagaaaa
    52381 ccgcgcagcc ctgctcccgg tgctttcgtc ccccctggtg gagatcgtgg agcgcctgcc
    52441 ggagggcgaa aggatcctgg tgttgcgaga ggcccgacgc ctgggagaga tgctgatgga
    52501 aaagggcatg ctcggggtgg gacggcgagc cccggagctc tgggaggtgt tggacgacac
    52561 ctccctatcc tggtcccaga agaacgtggc ctttcagctt ttggatcaaa tcttgctcca
    52621 tgctcaatat gacgatcgcc gcctgacggc ggccgtggag cgtcagcttc aggactcggt
    52681 gcagcctgtg gtgcgaaccc ttgacgtggg gcaggtcatc gtggagcagg gcgacgtggt
    52741 gacccccttc ctggggagcc tgctccggtc tcagggtttc cccgaggccc tttttccctg
    52801 gggtagcctc ttgctcgtga tggcggcatc cttcctttgg gggctctggc tcacttcctt
    52861 ccccatacgg gaaaaggcct tggaggaccg gagatctctg gtttttgtca ccttgctact
    52921 ggccgtttcc tggggagtgg aagtggggac cacctatttc cgcatggcgg gcatggggac
    52981 cctggtgctg gcggggtggt ccttcctcgc ccttccgccc tcttttgcct ttcagctggt
    53041 cctaggggga ggggccctgg gggttcttat ctcagaaagc ggcggtttgg cccatatttc
    53101 cctggggatg atggtggtgt ccgtggtgag cggcacggga ttcctcctct ttcgccaggt
    53161 gcgtagccgg gtccacctct ggcgggagat catcgtatta gggctggtga cctcaggggt
    53221 ggccctcctg gcgtggtggg gaagcggagc ccagatcacc gcgacgctgg tcctgcgatt
    53281 cctctccgcc agcgccatcc ttggggccct ggtggtggcc gtacttcccc tgtgggagaa
    53341 cgttttcgat atactatctc ccctgcgcct actggaactg agccatcacg cgagtccgct
    53401 attaaagcgc ctccaaatcg aagccccggg gacctatcac cacgtcctca tggtgggaac
    53461 cttggcggag agggcggcgg accgcctagg catgaatagc cttttggtgc aggcgggggc
    53521 gtactatcac gacatcggca aactcaagcg cccacagttt ttcgtggaaa atcagatgtc
    53581 cggggtgaac gtccacgacg acctttctcc ctccctttcg gccctggtga tcatcgccca
    53641 cgtgagagac ggcatggaga tcgccaccga ggcccggctt ccaaaaagac tcaggcagtt
    53701 tatccaggag caccacggta ccacccactt gtcttacttt tttagaaaag ccctggctca
    53761 gggggagagg gtggatcgag agcagttttg ttatcccggc ccctgccctc agtcccggga
    53821 gacggcggtg gtgatgctgg cggactcggt ggaggccgcc gtgcgggcgg cctggggggc
    53881 tccttccagg gaaactccgg gaggagacag ccccaggact ccggagggga aggacccttc
    53941 taggcaggac gcgatccagc gccccgcagc cattcagagc atcgtccaag gagtggtgga
    54001 gacgaaaatg cgggaagggc agctctccca cgtggatcta tccctaaaag atatcgcctt
    54061 ggtggaaaag acctttgtgg aaacgctcca attcatgtat cattcccgac aaacaaaaga
    54121 cgacgtgggt cccaaggccc cggaggggcg gtgaccccca tgcccatggc ggtggatttt
    54181 tacggtcggg agcttttagg agactcctgg tctttgcccc aggaggcgga gcgcgccttg
    54241 gaagacgttt tttttcagat tcttcgcgct cactgcggcc ctttggctcg ctccaagacc
    54301 tcggtggaag tttcgatcaa cctcgtatcc ccggaggaga tggccgaact gaacgaggcg
    54361 taccggggga cggcgggccc cacagatgtg ctctcctttc ccctctggga agagggggag
    54421 cgtttttccc ctccggtggg gtggaaagac ctccccttgg gagacattgt ggtgtgcgac
    54481 gaagtggtgg cccaccacgc cggagaacgg ggcgtccccg tgacggaaga actggcgctg
    54541 gtactagtgc atgggttctt tcaccttctg ggatgggatc acgacacccc cgataaagaa
    54601 atagccatgt ggcgggagca ggacggcccc gtccagagcc tgctgagaca gtggggcctc
    54661 ggggccccta aggactagag gaggtcgaaa gcgaacgtga gcagtgacat aggcagtagt
    54721 gtggccttac tggtttttct cttgattatt tccgccctgt gcagcgccac ggaaacggcc
    54781 attacggcgg tggggcgagc ccggctcctg gcgctccaag aacgtcagcc ccagcgccgg
    54841 gggctctacg gctggctcat gcgggagacc cagggggccc tcaccatgac gttggtggct
    54901 aataatgcgg tcaacatcgg agccagtgcc gtggccacgt cgctgaccat ctccctgttt
    54961 gggccttcgg cggtatggct ttccacgttg ctgatgacct ttgtcatcgt ggtcttcgga
    55021 gaaattttcc ccaaaagcgc cgccatggtc aacgcggagc gcttcctcgt gtggggcctg
    55081 cccgcagtgc gcctcttcca tgccattctc gtgcccctcc tctggttttt ctccggcatc
    55141 gtccgatttc taggaagaat cctccgggtg gacctttcct ctcagcgcac cttcgtcacc
    55201 cgggaagaga tcgagcaggt ggtctccatg ggagaggtct ccggcgccct ggaagaagat
    55261 gagcgccgca tgatccatgg ggtgatcgcc ttcgaagaga cccgggccta cgaaatcatg
    55321 gtgccccgga cggacatggt ggccgtttcc cgagatgcca gcgtgagcga ggccgtggaa
    55381 gtggtgcgtc gggagggaca ttcccggctt cccattttcg aaggcagcct ggatcgcatc
    55441 gtggggattc tctacgctaa agaccttctc cacgccctgg cggagggggc cacggacgca
    55501 ggcatcttcg aatttcgccg agatcccctc ttcgttcccg aagtgatgcg catcgcggag
    55561 ctctttgaca tcatgaaggg gggacgggtg cacatggcca tcgtggtgga cgagtacggc
    55621 ggcacggcgg gactcgtgac gctggaagac ctcctggagg agatcgtggg ggaaatccag
    55681 gacgagtacg acgaagaaac gccccccata aaacaagaag aggaagacac cttcctggtg
    55741 gaggggcaag taaacttaga agacctgggg gacgtgctgg gggactcctt ccacgcagaa
    55801 gacgtggata cggtggcggg actagccctt tctctggcgg gagagttccc cgaagaaggg
    55861 cagaccttcc gctacggcgc ctgggacttg gaagtcctgg gggtggaaga gcaccgggtg
    55921 aagctcttgc gcctccgcca tcggctcgaa gaacaaaaaa gcgaggagga agggcgatga
    55981 ccggtttttt gccgcccgga ggaccctggg gtgatgggtt ccccgaggcc ttgatggccc
    56041 aggcccgtca ggctcgagag agggcctacg ccccttattc gcgctttccc gtgggggcgg
    56101 ccctattgat ggaagacggc gccgtgctat gggggtgcaa cgtggaaaat gccagctatg
    56161 gcttgacctg ttgcgcggaa agaaacgccg tggggcacat ggtgctccgg gggtcgactc
    56221 accctcgagc ggtggcggtg gcgggccccc cgggggagcc ctgtttcccg tgtggtgcgt
    56281 gtcgccaggt cctttcggag tttaatcccg cccttttggt ggtggtggag cgtggcgaat
    56341 ccctggaggt ggtctccttg gaagagctac tcccccgatc cttcggcccc cgggatttgg
    56401 agagctcccg atgacttgcc ccggagatga agagcgaaaa gagggcggac cggagccttc
    56461 ggggcccctc tgtccctctg cggttctgac ctttcgatcg ggctttgtgg ccttgcttgg
    56521 acggcccaac gtgggcaagt cctccttggt gaatcgaatg ctggatgaca aactctgcat
    56581 cgtgtccccc gtcccccaga ccactcgcca cgccatccgg tgcattcgta ccacggaccg
    56641 atcccagatg gtctttgtgg acaccccggg actccatgag ccccgcaccg ccttagggac
    56701 ttacatggtg gaccgggtgc gaaagaccct ggacgaggtg gactgcctct gcctggtgct
    56761 ggacgcccgg cgtcctcaag ggggcgacga agaaaagatg ttgcacgctg tgctggccgg
    56821 ggcgacgacc ccgctggtgg tggcggtgaa caagatcgac gggatatccn nnnnnnnnnn
    56881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    56941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gagatgagat cccccacagc gtcaccgtgg
    57001 ccgtggagga gttcaaatcc cccgacgagt accacgaacg cgagacgggg tacatccggg
    57061 ccacggtgta cgtcgagagg gaagggcaaa agggcatcct catcggcgaa ggagggcgct
    57121 ttctcaaacg catcggcatg gcggcccggg gagaactgga ggcccggttg gggtatcccg
    57181 tctatcttga gctgtggatc aaggtccgag ccggatggcg ccggtctccg agggatttga
    57241 agcactttgg ctacgacgag acgtgagatt acagggcgtt cctgggggga agacgtgccg
    57301 ccccaattgg ggatgcaggg ctatggcagt gtcacggggg tggtattgcg gagggcggac
    57361 accccgaggg gggatcgacg agtcctcctc gccctaaggg gaattggccc cgtgtgggtt
    57421 tcggtccccg gaggggccaa ggggtccgtg cgatttgggg gggcgacgga accctttgta
    57481 tggggagttt ttcatctcta tcgcagcccc cggcgccttc atctccggga tgtggacgtg
    57541 aaggaacact tttgggagct ccggtctcgc ccggcctggc tgcgccgagg gctttcctgg
    57601 gatgcgttgc ttatccgagg ccttcttccc ggacacccct gcgacgaggt tcttcccccc
    57661 ttcttttggg ccctgtcttt tctccgagaa ggggtctcgg aagaggtggt ggagtggagg
    57721 tttttttgga agtggctcca cgcctgggga cgggctccgg atctggagag atgtgcctcc
    57781 tgcggagcct cgctggaggc ggaaggatgg gatccagaag aagaagggct ccggtgtgcg
    57841 gcctgtgttc ctcgacaaaa agagggagcc gcgaagggat ttcccctcct gaaggacttg
    57901 caccgggcgg cgtggctacc gccgaaagag ttcgcctcat ggacaccgga gaacttctgg
    57961 ccgcctcggt gggctcgggc aaacggatgg ctccggagca tcctcgtccg gagtgggtag
    58021 ggggaaagcg ggagttccgg gggcccttga atgccccggg ggaaaagatg tcgctggtct
    58081 tacgtgacag aggaggagaa ggggctgtga acctacagga tatcgtaatg agactggagc
    58141 gcttttgggc cgatcaaggt tgcgtcattc agcagcccta cgacatggaa atgggagcgg
    58201 gaaccatgag tccggccacc acgctccggg ttttgggccc cgaaccctgg agggtggcct
    58261 acgtccagcc ctcccggagg cccgccgacg ggcgttacgg ggaaaacccc aaccggttgc
    58321 agcattacta ccaataccaa gtcattatgc agcccgcgcc ggaagacatc caggagatat
    58381 acctcgatag cctccgggcg ctgggtattg acccccggga tcacgacgtg cgcttcgtgg
    58441 aagacgactg ggaatccccc accatcgggg cctggggtct ggggtgggag gtctggctcg
    58501 atggcatgga ggtaacccag tttacctact tccagcaggt ggggggcatc gatatgcacg
    58561 tggtgcctgc ggagctcact tacggcatcg agcgcatcgc catgtttgtc cagaaggtgg
    58621 acaacgtcta tgacctctcc tgggtgggaa aggttcgcta tggcgacatc caccataagg
    58681 gcgaggtgga acattccacg tataactttg acctctcgga tacggccatg ctctttcaac
    58741 ttttttccat gtatgaggcg gaggcccggc gcctggcgga ggcggggttc gtccttccgg
    58801 cctacgacta tgtgctcaag tgctctcata cttttaacct tttggatgct cgcaatgcca
    58861 ttagcgtcac ggagcgcacg gggtacatcg gccgggtacg gagtttggca tccctttgct
    58921 gtcgtgccta cgccagacag cgggaagaga tgggatatcc cctgatgggc aagttcggcg
    58981 cataaggaga ccatatcatg aacgaacgag atctgttgct ggaaataggc acggaagaaa
    59041 tcccctcccg cttcgtggcc tgggcccggg aagagctggc atcgttggcg cgccaggaat
    59101 tcgcctccgc tcacctcaag tttagggatg tgggggtcta cggcacgccg cgccgtttgg
    59161 cccttttcgt ccggggggtg gcgataaacc aggaggatct ggtgcaggag cacaaggggc
    59221 ccgcctgggc tcaatctttt gacgtctacg gccaccccac caatgcgggg cggggatttg
    59281 ccaaaagccg gggcgttccc gtggagagcc tggaaaagcg cgtggtcgaa ggggtgccct
    59341 atgcctttgt cctcacccgc atagaagggc ggcccacagc ggcgctcctg ccggagctcc
    59401 tggaccgtct gcttcgccgc atcgtctttc ccaaaaacat gtactgggac gacccgggca
    59461 cccgctttgc ccgtcccata cggtggctcc tctgcctctg ggacgacgaa gtcatccccc
    59521 tcacctttgc ggggtgtgtg gcggatcgct tcacctcggg acaccgattt ttgggacaaa
    59581 agcgggtgga ggtcccttcg gtgcgtgcct acatggactt gctctacgat aactacgtca
    59641 tagtggatca ggacaagaga agggagcgta tgacgggggc catcgccgcc ctagaaaaag
    59701 agatcgacgg caaggcggac cttactcctg ccctggtgga agaaaacctg gacctggtgg
    59761 aatatcccgt gccctttttt ggcacgttcg aagatagatt cctcgacatg ccgccggagg
    59821 tcctcaccac caccatggcg gtgcaccagc gatactttcc ggtccacaac ggccagggta
    59881 agctcttacc cctcttcgtg ggggtgagca ataaccgggc tacgagcatg gcggtggtgc
    59941 gggagggcaa tgagagggtc ctgcgggccc gcctttccga tgcggcgttc ttttgggcgg
    60001 aagatcaaaa gcgccccctg gcatcgcggg tggaggagct cg
//
LOCUS       DAOOER010000007        51924 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_690_length_51924_cov_9.058474, whole genome shotgun sequence.
ACCESSION   DAOOER010000007 DAOOER010000000
VERSION     DAOOER010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 51924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51924
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_690_length_51924_cov_9.058474"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            366..539
                     /locus_tag="PL162_01805"
     CDS             366..539
                     /locus_tag="PL162_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90795.1"
                     /translation="MRNMKDMKELFEMALGLGAEWHVTGITLSPEEHRIDIALDFDRG
                     TKFPCYKSTVFLS"
     gene            578..787
                     /locus_tag="PL162_01810"
     CDS             578..787
                     /locus_tag="PL162_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90796.1"
                     /translation="MKQTVEAIIDERGHVRLTEPIFITGIRRALLTVLDEPPSALDET
                     LLSAEQSLAADWLRPEEDDAWAHLQ"
     gene            769..1125
                     /locus_tag="PL162_01815"
     CDS             769..1125
                     /locus_tag="PL162_01815"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HQF90797.1"
                     /translation="MGAFTVGSVVLVPFPFSDLSGSKKRPALLLADVGRGDWVCLQIT
                     SNAYGDCTAILLKDEECAEGFLARTSTIRPGKLFTAHESLFIRCVARIADSKMDEVRQ
                     TVLTLIKNGRFQEHIS"
     gene            complement(1234..2883)
                     /locus_tag="PL162_01820"
     CDS             complement(1234..2883)
                     /locus_tag="PL162_01820"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HQF90798.1"
                     /translation="MKYRSWLRALAVATLVTVSAGSAWACTVFMVGKNATVDGSVMTS
                     HTCDGWYDQRVQIIPGGPHKAGEMVPIHKNICYQTRPKAELQLVGEIPQVPETYTYFH
                     VGYPFMNEKSVVMGEYTWGGRDENLCEEKGWMMIEQLQALALQRAATARDAIRIMGEL
                     AEKYGYADGGEAVSVADKNGEVWLFEICGPGPLWTPESGKPGAIWVAQRIPDDGFAMG
                     ANRARIAEIEWDNKNDFMYSSNIRSFAQDMGWWKDGEPFVFHKIYNPNPYGSPYYQQR
                     REWRAYTLIEPSLKLDPNLAEQYPLVIKPSKKLSVQDLMAVYRDNLEGTPYDLTTGLA
                     AGPFGTPTRYATPKGVRPENRKGNDWDRSISLFRCSYSFVSQVRPDMPDPIAAVLWFG
                     EDQPSTTLYMPIYAGTTEVPRPYAVGKRHEFDRDSAWWAWNFVGNWADLKFSHMIQDI
                     RQVQQQLEGKYFLELPQADKKALEYYQKDPKLAVDFVNRFVNENLTEGLDTWWNLAWH
                     LVGKYHDGMVISPDGSFSTPGYPTWWLEKVGFGDTDAEPKH"
     gene            complement(3040..4209)
                     /locus_tag="PL162_01825"
     CDS             complement(3040..4209)
                     /locus_tag="PL162_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HQF90799.1"
                     /translation="MESRNILIKLLTLAVAGLCAFVAGKEFLEHRNYLEPMVAGPGVT
                     EVRLLSSYSPAVRDTINDCHVYILDSGKPGATVMVIGGTHPEEPAGVLAAAVLVQNAH
                     LDQGKLVVVPRVNRSASTCTRPGEAYPTYFHVKTPWGVARYRMGDRCSNALDSWPDPE
                     VYLHYPSRQMLAYMDIRNFNRTWPGRANGLITERTNYAFMEVVRQEKVDIFIDYHEAE
                     LEYPVENTIVTHERGQAVAAMTSMMLTAQEFPVPMGMEFSPKALHGLSHREVGDYSEA
                     MSMIFEVAEPMLDRIRGITDEKLLMEGRDDFVVKAGEHKLLYAPMDDQGWPIDVRVGR
                     HLSALLQTIDSYNMTAPDKPLLLSGVPRYAEVIEKTTGVFLQNPSAASADRVYYD"
     gene            complement(4222..4389)
                     /locus_tag="PL162_01830"
     CDS             complement(4222..4389)
                     /locus_tag="PL162_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90800.1"
                     /translation="MLVQMNEIVTLLYYALTGIFGLVIAWNFVKTRNVQDAVLYAIVL
                     VPFVLRLLRVK"
     gene            complement(4394..5698)
                     /locus_tag="PL162_01835"
     CDS             complement(4394..5698)
                     /locus_tag="PL162_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate ABC transporter"
                     /protein_id="HQF90801.1"
                     /translation="MFVHASLVLAVIVGAFLAAKKFQVSTELSMLLAAVAGGCVHGVM
                     NGDLSSLVRHLVEGTFTYFDVCLIFITATFFMALVKETGGVAFIVRKIVGTFAERRFL
                     CLLLLTLVMLIPGALTGSGAASVLTVGALVGSVLAAMGVEETRRVAIIFLCAAMSAAA
                     PPINLWAMMAAAGANMPYVGFAAPLAILSVTGALFSMFWLAGGTKAIDRDTALGALPE
                     VPEGMTWGRVLFPFGLLALLVGAGRLLPFHMPVLGLPLIFTLCALVTVVLSPRRLPLG
                     RIASETIQSLLPLVGIMVVVGSLIQIMAWSGARGLISLSVVTLPLSALFATLFLILPI
                     SEGMLQYAVAPLIGVPLVMLFNMKGLDPVVALSAMAVMWPLGDCLPPTAVVGRAAVME
                     LGYQGDYYGQFVKSCVVPMAFISVLGTLFLIYSKQIADVLGG"
     gene            complement(5790..6344)
                     /locus_tag="PL162_01840"
     CDS             complement(5790..6344)
                     /locus_tag="PL162_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HQF90802.1"
                     /translation="MKKAVWGFFMIAAVLLAAAGAWAAVEPPLVITTCGQSPGAVMAK
                     MSCLQAKLSPTESDNQLSAEGIKGKGYKTLMVTTGTSLKGMGAAGTDVDKEIDRVRAL
                     IEAARAEGMMVIGAHIEGMARRTDASDEASIKAVMPLADMILIVADSDKDGFFTTYAK
                     ELNKPLLVAKDALGVGATLAAYNK"
     gene            6573..7553
                     /locus_tag="PL162_01845"
     CDS             6573..7553
                     /locus_tag="PL162_01845"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HQF90803.1"
                     /translation="MGHRVLVTGTRGKSGMVRLLDAALRACGVRCWSRITGVVPTVYS
                     PEGAFRLRRTAPASVEEMRWWLATLPRNAEAVVLENSAVAPELQPLAGRWLRPTLTVW
                     TNAHPDHQEVWGAGSEDALRVLFQGIPAGVPVVLGRELGALPELLRALERQGCAVSVA
                     PGSEEDGVLERQRATALAACAVLGLDRGRCLEGMGALAPDPGDFRTLRRGDGVLASAF
                     AANEPTSSEGLFASLRWRREDTVLWYHHRRDRSGRLEAFLPWFRRGWKEIWLTGALPW
                     RPVAGARILGNPPPEEMERRLGEGLRIFGCGNVAGLPLALLTRWKGGFPA"
     gene            7550..7966
                     /locus_tag="PL162_01850"
     CDS             7550..7966
                     /locus_tag="PL162_01850"
                     /inference="COORDINATES: protein motif:HMM:NF025467.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein
                     PgsC/CapC"
                     /protein_id="HQF90804.1"
                     /translation="MTEEMGLFFLALLLAGIYSHRTGWGTGGVVTPGFLALHAARPLF
                     LAGMLGLGVLLALMLEVLVRRFSLFGRERLTVALVLSVGALGTLGALFAVPLPWYGWV
                     IPGLVAADCQRQGALGTLGGVLACGGAALCARGLLP"
     gene            7963..9081
                     /gene="pgsW"
                     /locus_tag="PL162_01855"
     CDS             7963..9081
                     /gene="pgsW"
                     /locus_tag="PL162_01855"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HQF90805.1"
                     /translation="MRPSATDFSWRSLWRGWGPGFFRWWICGEPPFPLCLALLALGLA
                     LLVWGPWSGRAGGIEGKALGTVRRAQDYVVAWRTERGIPPSPESDPWHTGLVGEEMTA
                     LTTTLGDVAAKRTACDPRWVPVLFRWFRKAGLTSGDRITVLPSGSFPGFLLATLVAGE
                     AAGLRIQLLPSLGSSSWGANRGDLTLLDLLALWRRGGFVTTRVEACTLGGGEENARDM
                     PQEGVELLREAARRHGVPVLEAESWRALRDLKAARMEAFSPRLVVQIGGGMTSLGDDP
                     AVLEVPPGLLLPEEHAPRGDGLLAWALERRIPAVHLLDVRTLARREGVPFDAPPDAAF
                     GRMAPGPALVGLLLFALVLGTHRRWSLEPLETFRSPGD"
     gene            9585..10361
                     /locus_tag="PL162_01860"
     CDS             9585..10361
                     /locus_tag="PL162_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQF90806.1"
                     /translation="MKVRRQMISIDEEKCNGCGLCARACHEGALRIVDGKARLVSDIY
                     CDGLGNCIGACPQDAITFEEREAEAYDEEAVRRHLAGETHQAPEVTPPPLPCGCPGTA
                     ARSLRDGAKAPKTEGEVPKGGAPSLLENWPVQLRLVPEGAPYLQGARWVLAADCVPFA
                     LGDFHARFLAEEGTVCLVGCPKLDDAGAYRDKLARMIAVSHPREIVVVRMEVPCCGGL
                     SRLLEDAVEEARVDLPVTTVVVGLRGDVGPGEVTRYRFRQ"
     gene            complement(11386..12771)
                     /locus_tag="PL162_01865"
     CDS             complement(11386..12771)
                     /locus_tag="PL162_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HQF90807.1"
                     /translation="MAVVPAHIFREYDIRGLADEELTDATVRLIGRGYGTFLAASGIR
                     RVPVAGDVRLSTPRIRAAALEGLRDAGIDVVDLGYLTTPAFYWSLYHLACDGGIMVTG
                     SHNPKEFNGLKLAFGKLTLYGDQIQEIRRIIEADAFATPAARGTLEQAHIGDAYVEML
                     LEKISLGPRPLTVVLDGGNGTAGLFMEKFFGRLGCRVIPLFAEPDGTFPNHHPDPTVR
                     ENLPPLIEAVRRHHADVGLGFDGDADRLGVVDDQGEVLWGDQLMALYWREILPKHPGA
                     DAIIEVKSSQLLVDEVVRLGGKPQFWKSGHSLVKARMKEIGALFSGEVSGHMFFADEF
                     FGFDDSFYAAGRLLRLLSHSPEPLSALVASLPRYFSTAETRFPCPDDRKFAVVERVKE
                     EALRDFDAVTVDGVRIIYPTGWGLLRASNTQPILVARAEGKTPEDLERNAKDLQARIE
                     RAGGGSFEWTY"
     gene            13011..13940
                     /locus_tag="PL162_01870"
     CDS             13011..13940
                     /locus_tag="PL162_01870"
                     /inference="COORDINATES: protein motif:HMM:NF015509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HQF90808.1"
                     /translation="MNGLRMVSPLVIVMALGYFLRRRGMLEGDVTFRMNRLLFYVALP
                     AWLFRSILRAGAAPMDHLHLFTACHVGFFLVPALAWLLALPFREPRERLGVSVLSAVR
                     SNNIFMGVPAIGLALGEPGLEALSLFLGLSFVGYHMISIGWAQLALSGELTLRALKEM
                     GRGVIRNPLVQASAAGMGLSLLGVKALPQELDVTLKIVGDVASGLALITLGASLRPER
                     FWRGLRHSWRDVSVKLVLHPLAVWALFRVWPVAPLMEQVVVIVAAMPVAVNTFVVAEG
                     MGMDAPYTAELIAVSTVLSVGTLPLWMGVLGMG"
     gene            14001..14369
                     /locus_tag="PL162_01875"
     CDS             14001..14369
                     /locus_tag="PL162_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
                     /protein_id="HQF90809.1"
                     /translation="MDAVLVFLLAGDIIANAGAHVLLKQGAQKPRSQAPGLRGMVENI
                     LFNPCSLVGMGAYVLSFVFYAALLTRMDLSVAYPLCTSLSFLMVLLASVLWFRERLDG
                     VRLGGIGAILLGIVVISIGG"
     gene            complement(14469..16103)
                     /locus_tag="PL162_01880"
     CDS             complement(14469..16103)
                     /locus_tag="PL162_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90810.1"
                     /translation="MNSIKRRSIGAVLLLLVAALWAGGALAEERYQPADAIGPWSDES
                     FHMAGNVSDVQKLVKSIVESPLLALDPSSQEGIQALSAVNAMYPVEGFAFGVGMQKSV
                     LRLQLAVSVGPARADLLSRIAKGAMISEDLELLTQFGVPVEGPLSLEDGTTAYRVPLE
                     GTPFALHFAAQGNLFLLGFRPEDLQESVAALKDPAQRFAPQRHFPDSPNYFLFRDNGL
                     LQIALMAAEVPYLLRDNVLMEFSCAVKENQWSLGIFTNLARALFSPKALEAFKSLSSP
                     LPLLGGGKVLGAFHFSEDYDHLKSVLQNNPDEEVRDGFEEFLQGVQEMGLSEETLKSL
                     LTGSVGAVLGGTASFASAPLPGAYLVFEGQGDGAKTVADLLVKTLQAQEMPLAPVTIP
                     GWDAVYGMPLPVTLTIAHRGSTLLVGLLDANQLATPAEGSPRLQELQGDASLYNYLYI
                     DNEALQKELLTFLKGADIWRSILEDGMDEVGAAIVQAHAFIATVQDLVVKCSGLEKGS
                     IDLNFFAPDGAQEAEWEKMKGSWGAPQGEEPKPASN"
     gene            16438..17274
                     /locus_tag="PL162_01885"
     CDS             16438..17274
                     /locus_tag="PL162_01885"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HQF90811.1"
                     /translation="MHQIFVFSGTGNTWHLAKRTGQLLGDAELVFLSGLRHDAAVLAQ
                     GDTVGIASPVYAGGLPSLVKSFLAKVVLRRGAWSYLLLTHGGRPFGAGRQGARILREQ
                     GVALDAVFSVAMPSGFPPRLGGIFARRPEGRLAREEESAAAVAEAVRERRGGGIEGAF
                     SWVTDLFHEGLLARRRRLPFRAEGHCTGCGTCREVCPVENIVMDGMHRPRWLGSCEGC
                     GACYAWCPVGAIRVGRFPWGGEPYHHPGVSLGEMRGGFRKKPFRVSRREEGPFPGDVG
                     GI"
     gene            17337..18125
                     /locus_tag="PL162_01890"
     CDS             17337..18125
                     /locus_tag="PL162_01890"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HQF90812.1"
                     /translation="MMHQIYVFSSTGNTLHAAERVGEGLGGARLINMALAQDKETEAS
                     GDSVGICYPVHAFGAPAVVHRFIEGLSVDPESWVYLIVTSGGGPLGAAAQGVGLLAQR
                     GVRVRGGFHVKMPGNYSPLHDSPSGGKLRRMVDRADGVLEGIVEDLRNRREKRPGGSS
                     MEWLSRWANGSALASVGAKDRSFYATSSCVRCGRCVALCPVGNVEQGEEGLPRWMGRC
                     TQCMACFHWCPVHAVQFNSTTSLRRHRYHHPAVSPERFLAWSGR"
     gene            18147..19475
                     /locus_tag="PL162_01895"
     CDS             18147..19475
                     /locus_tag="PL162_01895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HQF90813.1"
                     /translation="MDLARKVEILGAAARYDASCASSGSSRRGTEGGMGAASFYGVCH
                     SWSDDGRCISLLKILQSNACVYDCAYCANRASGNAPRATLTPEELAELTINFYRRNYI
                     EGLFLSSGVVGSPDATMELMLRTVSLLRGTHAFGGYIHAKAIPGASSELVSRLGRLAD
                     RMSVNIELPTEAGLRRLAPAKTREGILGPMGFLAEHIKEAEEDRARSPRAPLFVPAGQ
                     STQLIVGATEDSDLQILRLAQGLYRRYALRRVYYSAYVPVGADDRLPPIVAPPLRREH
                     RTYQADWLLRFYGFSAEELLDEAHPRYEGDLDPKAAWALRHLDLFPVEVNTAPLAMLL
                     RVPGVGVKSAKRIVAARRERRLDALALPRLGVVMKRARYFLMANGRHCGGGTMDPAFL
                     RPRIGDGKESPWGRQLSLFADAGEAKRGPEALFPAMAFPDRRDPVAAGGA"
     gene            19477..20244
                     /locus_tag="PL162_01900"
     CDS             19477..20244
                     /locus_tag="PL162_01900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HQF90814.1"
                     /translation="MALWAFDGSFEGLLSTYAEIFQRQETPDDVVVQGEGSQGTLFSP
                     AFVPSDEKKAEAFARAVRRRLGDRTFDTVMVAFLSERPRMAWHLWRYLDLGRREGPAF
                     FLQRGDPRVSSVHLARRAVLREVHRLSGLARFEPLQGVLYAPLAPRYDVLELLGPLFI
                     PRLSGERWMLHDVGRGKAVCGEGGELRGVEAPRRALKVVSEGERDYRRLWQRYFEIIT
                     VEHRRNSRLQKHFIPERYWKFLTEKALEPEAFSPGPP"
     gene            complement(20268..21230)
                     /locus_tag="PL162_01905"
     CDS             complement(20268..21230)
                     /locus_tag="PL162_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90815.1"
                     /translation="MEPQDALNRIKELARQDPTLLNDATGERMAHLLAFVLRESQPGR
                     PIASLAEGASGGGERSPDSPPPTVEEVLSLLRREQALRRAPLAPGKEGERPRPGAGAP
                     FDAPSRIVALEDAFRTLREQTAVALEDLRQESRQALHSHAKQISHRLGDLQREAAGQH
                     MTILSRFVALEARLEDRSHRELHLHELVADQRNDLEALMALVVEEGKALRREMGQAIP
                     PKDQIPPREETSPAPGGVPPEKDPLPVDPRSLYQDLRRDLRETQILVARYHTRLTREL
                     VTLRRSVEDLEGRLGRRDDPAPLSEDPLPPAPPPRRLLEPRREG"
     gene            complement(21289..24084)
                     /locus_tag="PL162_01910"
     CDS             complement(21289..24084)
                     /locus_tag="PL162_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90816.1"
                     /translation="MEPQDVLKTVRSLAHLDLSRMDEGTREQVASVLTFALESLRKDW
                     LSPEEGAQELRVSPRRFRAILKRYQILEQQRLALENIEREEERFIDTIRELTPSPSEG
                     VSGTPAMVSAPEEPDPLPRASGGNDAPPEEASPSPEETPAEGKRAPQPAAPAEGTPPA
                     EIVEEAPETSPSSEDPEEDPFTDAPSEGPSEAEKYFSLEEESAQMAQALFAAVQHSYD
                     DELRRLRLAVERLSSDGASHRHQFTERLEIMNDAYHKALDSLVARATEERHRGEEFFS
                     SRCEDFARSVHITAANLREENAVLRNKFASALREWTEENDALRHRFAAALQEWIEDKE
                     RYALALRESADRCRLLEERMETLRQEGLAARGEDRARQEAREAQIDQTLGTLEERWQE
                     ARGAAEELNRRLDRETAELGRRMDQGTAEMSHRLEQSSAELNRRLEQETAELRQRLDQ
                     GQGDIAQRVETLRQEDRAAGKEERRRFEEKNALALERLRKEAEDALRGHRQSLREEMD
                     ALRSHQQEELQAALTLVAGENGRLMEEMDRRQREAGELWDREISRNHAALSQKIQHLA
                     QDLGALQERSQTREAAWRLQDDALQGAVEDLRKEMARFQAQLIARESDRDGAMAEEGR
                     RREEELHRSLEEALQDVRRRIDAGDQLREERHGQMETRWKAFLEETSRETARQAEAIA
                     RIREEKMSSNVEIAKSQQFQGQMLKMIKTDLMALSSEAKKDLAQLKERCVHLERHRTG
                     EEGRFAGEIAAVERRLAAVDALKSALQGEVQKREVLTEEMDRLGRYQQGLSQDLQDRE
                     RREAEERDALAKATAQLAETVESFRDKGEALEREVALHRKALGKDFDALRESWKRMGQ
                     ELALQQKTFERGLDSLRQEGLDRQAEERHRREELASELRSALGAMADHVEKLRVSQAP
                     PPPKGAIDWLFGEKPRE"
     gene            complement(24232..25056)
                     /locus_tag="PL162_01915"
     CDS             complement(24232..25056)
                     /locus_tag="PL162_01915"
                     /inference="COORDINATES: protein motif:HMM:NF017941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HQF90817.1"
                     /translation="MYEVFSMWGKTKMVVLVALCAGLYAALLIPFKGFVLIPGVTEFR
                     PASALPVVMGLLFGPAGAWGSAIGNLIGDFFGSLGIGSLFGFVGNFFFAYVPYKLWIN
                     LGLVPSQDREPHPTSRRKVVAYVVVSFLGSAGCALPIAWGLELLGMVPFGALGSIIVL
                     NNTIPAVVLGLPILTVLYPRIKKWDLLWTDIMDEHEIPVGGAMSLIGGFFMTLSILLG
                     MAGGFLAASRAGQGLLYSGFGAGGIVGSLGVVLVAGIGTAGLVLSSFIQSMPPKKR"
     gene            complement(25158..25940)
                     /locus_tag="PL162_01920"
     CDS             complement(25158..25940)
                     /locus_tag="PL162_01920"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HQF90818.1"
                     /translation="MDLFLYMDRDTAVHRLDPRTKILLLLVSFALALAFSTLPPLGVL
                     LALVLLHGAWGAVLSNLRRIRLVLAMIALFSLVIWSIVGPGKSAGLAFSWEGVLYGLM
                     VAAKTDLMIIAGMVFLSTTKIEEISQGLVKLGLPYRGAFAFSTAIRLVPLIVATSATI
                     AQAQKSRGLDLDAGSFLEKIRKHVPLVIPTLISVIRSTNVFSMALESRGFGYSPQRTN
                     FLELRMKGGDYLTVAFALALAAVGLVAKFFPQALESWGAILP"
     gene            complement(25940..27661)
                     /locus_tag="PL162_01925"
     CDS             complement(25940..27661)
                     /locus_tag="PL162_01925"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HQF90819.1"
                     /translation="MDDSGPPRAIAVQGLSFSYRGAEGLPALRDVDFEVLPGEFVALM
                     GPGGAGKSTMAHALNGLIPHFVRGSVSGEIRVGGQDPRHTPVSHMAREIGLVFQDFEA
                     QIFSTNVDLEVAFGPENFAVPPEEIARRVEGTLRTVGLSGFAGRHPATLSGGQKQRLA
                     IGAVLAAHPRVICMDEPTTDLDPRGKEEVFTIARQLHDSDGLTLVIIEHETEEALHAD
                     RLVLMKDGRIVAQGRPADLLRDVELIEGLGIMPLAIPSYFAGLALGSEDPLPLRPGEG
                     AAFFHRQELRLDEGRVQELRERDRKRQESYGAVVVRVENLSHTYPQGTRALDGVSLEI
                     RQGEFVALLGHNGGGKTTLAKHFNALLTPTEGRVTVCGKDTRTHSVFEIGQDIGYVFQ
                     NPDHQIFSPTVHDEVAFSPRIRGVSPEEVKLRVREALEAVGIPDYGPQDPFSLSKGER
                     QRVAVASVLSARPKIIVLDEPTTGLDYSGQRKMMELIKKLNEAGHTVVIITHTMWVAA
                     EYAHRVVTMKDGRVGHDGPAREVFSREKELAAWGLKTPSIVSLGNLLGAPVLSAEELA
                     YCTKEGR"
     gene            28145..28348
                     /locus_tag="PL162_01930"
     CDS             28145..28348
                     /locus_tag="PL162_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90820.1"
                     /translation="MHGIATLQKHARQEGHKEGIEKGRREGLRASARNMLRKGMPMAL
                     VAEVTGLSPDEVAAPRDCVNSQQ"
     gene            28429..28782
                     /locus_tag="PL162_01935"
     CDS             28429..28782
                     /locus_tag="PL162_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90821.1"
                     /translation="MTGFKEKVQLHRTLLRRGVSLMKGGGLLLLLFLGSTVMLGGCGG
                     GDAAHRETLFVNGAIYVDADSKVDNLLIRDGIVAGHNVDPNEHPEASVVDLNGAAAYP
                     GFNDSHVHLVSMAVA"
     gene            28786..30213
                     /locus_tag="PL162_01940"
     CDS             28786..30213
                     /locus_tag="PL162_01940"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQF90822.1"
                     /translation="MLAESSALREGKERPRPEDFFDDIRIRCSQVPKGTPVMSHGFVL
                     DDYDAWGLADLAALDAASGPDHPVMMADQLGHSYIVNSAAMRISGLDADTVAPPGGKI
                     VRDPATGAPTGMLRETAGALVGNVAIFPQVPDQIVREPLLKLLHLWASMGYTSIVELM
                     GGPMGRTMKPGLLRDLEGEGKLPLRVCYAYTFTSLNDLEGYKDAGPDTDLVHFSGLKL
                     FVDGAAGQGGAWTSWENQLEDPSQRHGLHAVLTEEVEGLAAYNIFSIVDRSEELGLDV
                     HYHVGGDLAIQAVLDAINAAKKKNGRFKGSHTLYHLGFLTDPQIEEMKLLGGHVIAGI
                     QPSLHWKNEWKNTERFYGPYARSAYPYQKMLDAGIRLAFSTDFAPNTVLMSYPTEIMR
                     VVLTGGEGRRQNTISMRDLIEGFTSGGFATTPLTNTGKLHIGYKADIVVFEKDLYHVP
                     PQQLSKDYPRVLGTWVGGRKITADR"
     gene            complement(30615..31601)
                     /locus_tag="PL162_01945"
     CDS             complement(30615..31601)
                     /locus_tag="PL162_01945"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQF90823.1"
                     /translation="MTREELLQREYYGRVERAVDHIYAHYGEDIGLADLADVAGFSRF
                     HFHRIFSGIVGETVGEFLKRIRLQHAAARLFDRPEESVTEVALAVGFSSSSVFARAFR
                     ERFGVTASQWRRRGRTTNPPRDQSNRDQTDRTEDQAEGNGEQARSAEGRYYSFTLSDE
                     GKRRKIKMAKLAYTVEVKELPELTVAYVRHIGPYSGIPAAFERLSRWAGPRGLFGIPG
                     AVTLGVYHDCPETTEESKLRSSACLTVPPDTAVSGDVNLMTIPGGTYAVARFEILPHQ
                     FGEAWETFMGDWFPSSGYQPDDRMCYEIYRQDPQNHPEGKFVVDICEPVRPL"
     gene            complement(31756..32976)
                     /locus_tag="PL162_01950"
     CDS             complement(31756..32976)
                     /locus_tag="PL162_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQF90824.1"
                     /translation="MLEVLRTAVRSLMGNKARSLLTMLGVVIGVGAVITMVALGQGAG
                     ARIESSVASVGRDILIVFPGAPNTSGVRGSAGSGSALTRDDGEAMALECPSVAAVAIE
                     TFGRGQVVFENMNWSAPITGTTPPIFDIREWRLAEGRFFGDGDVKNGAKVCLLGTTVA
                     ENLFGHGEVVGKTVRIQKTPFEVVGVLAPKGQTPWGQDQDDVVLMPLTTAQRHLFRSS
                     IPGAVRRLTVKARNAESVLTAQEEITVLLRQRHRIPHDGDDDFVVRNMTDILEASAES
                     TRIMSLLLGAVASVSLLVGGIGIMNIMLVSVTERTKEIGIRMAVGARPGDIRWQFLTE
                     ALVLSLLGGGVGILLGAGASRGITAWLDWPTQISPPVALLSFGFSGLVGVFFGFYPAW
                     KASLLDPIEALRHE"
     gene            complement(32979..33722)
                     /locus_tag="PL162_01955"
     CDS             complement(32979..33722)
                     /locus_tag="PL162_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQF90825.1"
                     /translation="MTSPGTQGGAPEIPPLLEVRGVTKIYRTGDGELRALDHVSFTVL
                     RGEFVAIMGHSGSGKSTMMNVLGCLDSPTDGTYRLDGRDVGHLSRDELARVRNEKIGF
                     VFQGFHLLPRATALENVELPLLYAGVSSRLRGTRASAMLDRVGLEHRKGHRPNQMSGG
                     QQQRVAIARALVADAPLILADEPTGNLDSHTSDEIMELFAALHREGKTVVLVTHEADI
                     AAWARRVLRFADGRLVEDLSTPGRPPKEA"
     gene            complement(33719..34951)
                     /locus_tag="PL162_01960"
     CDS             complement(33719..34951)
                     /locus_tag="PL162_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQF90826.1"
                     /translation="MKKLLIFSLVVALGGGGGWYALAPGEEGVQFATVPLERQDMVST
                     VTATGRIEAVSVVAVGTQVSGTIMAISVDFNDRVHRGQVIALLDPSILQSQVEQQEAN
                     LSLAQAGVARASATVADAERTFRRQKELFSRRLIAQSEVDAAETTLLTARAGRQEAQA
                     KVAQARASLKQARENLGYARIISPTDGTVIDRKVDVGQTVAASFQTPTLFSIAEDLTR
                     MQIEAHVDEADIGRVTEGQDVEFRVDAHPGKLFRGTVTQVRLAPAVTENVVTYTTVIR
                     VDNEELLLKPGMTATVTIITERRPQVLRVPAAALRFAPPEAPSSSASGAMPGMGPTFP
                     RRPNGGRHGAGRSGPRIWVLSEDAPWPVAVVPGISDGSFVEILSGDLREGQPIITSAT
                     KVPGTSRWQRLLASGGGP"
     gene            complement(34948..36243)
                     /locus_tag="PL162_01965"
     CDS             complement(34948..36243)
                     /locus_tag="PL162_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQF90827.1"
                     /translation="MTHFPRKISGGAFRLRSGPGVLLALLLFASGAEADRSDPPVLSL
                     EACLAMAEAHHPELRGAEAEERMERARLRQIAARKGVLVDATATESHPARGEASHGLG
                     IAAGVKIYDAGRDHREEEAQELAVEGARWKRINLNQTLRAAVKRAYIDLLLAEATERQ
                     AQATEKAFEDHLATARGFYEAGTKSRIDVTKAEVDRGNAHTALVKARSARDLAEATLV
                     NAMGISSMPPFKTVDLSPDLSPVPPEAELWAQALERRGDLRAARLQERAGRLKTEAAA
                     RGYAPTARLQGSWGYEGARFPLEENGRVSLTVSWPVADGGATRGATEESRAREDSLAA
                     ASEVLRQRIALEIRRALRSLEEAEAQRENQELVVRQAEENLALATGRYEAGVGSALEV
                     TDAVLALNDARLASYRIRYGAAAARADLEQATGGKEPIP"
     gene            36567..40025
                     /locus_tag="PL162_01970"
     CDS             36567..40025
                     /locus_tag="PL162_01970"
                     /inference="COORDINATES: protein motif:HMM:NF024516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3971 domain-containing protein"
                     /protein_id="HQF90828.1"
                     /translation="MARKKVVGLVIGALLAVVVGGVFFVDGATDLVKDQAQSAVRDSL
                     GGELLLGDVTGNPLRGYTVTRVALEKDGVTLFEADRISLAVSLLAFLRGGAIISDVEI
                     AGARGDADRLGRFFATIKGEGGGGSSLVGRVTFSDSVLTTPAGTLLASRARISLGGST
                     VALELDGRWQGPGKTPLSLSGKGTLAFQGDALAVAELDLRVGQGKVLLQGSVLPELGL
                     WGTVEGLEGSEVGALWPETEGALRGRLSLAIQSGGTWKNPRISGDATLEGGKFSGVTV
                     DRASGRFSFTPALLKVAGIEASVFGASLRGTLALGLRNLPPTVALQMRGAKGDLAQVA
                     QAFPALGDLQGMVEKISLDLKGSGSALEGRMTLVAPSLSGRGHKLEQVAADVKLRDGK
                     TFHVAARAQGFGSPLTLSGTASWGKKIDMNLEFKATRLDLARLEALFPELRELKPRGE
                     ASVAVTLKGDPGAPQISGIVSAPQIILAGETLSKPVVKFALRGNTLAVNAASLTWKGG
                     VFSASGSVGGLGGKGVTLDLKGKGEALALENLAGAFPALKGSGVKGTGTLSWKASGSP
                     EKILVEAALSWKSLRALDQLGVSDLEIRGSGRVTGGKPALEGPLTLRAARLAWAGAGV
                     DRPTLALRAEGNRLVVERGEGRIAGGSLALSGTAKLPERAGEEGALDFKGSIRDLGLG
                     ALGKDLGLPVALGGQGTVNFSLGGGLAAPRFALDATFPTVTVEGAALADASVSLEGTP
                     GEIRLTSARGAVEGTPFSFSGMMRPGPQGYALDLKGSGEGLDLASLLRRVPALRDKGI
                     QGKMNLRFFLKGTPGALEGSGELTSERVGFLGLSATGVKAPFSVQKNVLAAPAIKALF
                     HGGDAALGLRMNLGTGGWELQAALAEVDLGATLRALSQASGTVGGTATVDFKGKGNAF
                     KGEFSGKGLFQSREGAVTGYPWVNLVATLHGASGVRYQLLKAPFSVQGAQLTLQEGTK
                     AVPWEGDPLYRSLEAQGTVGPEGKLNLAVKGVVNAQVLNALTGGVQGGLLEGIGGGAS
                     VKDILKGALKGFADGGAKQDFRAVSLKVGGRAASPKISDLQVESKAPGQGDGASPSPE
                     KPAPAPEKTISLPGGIEVPLPDGILPEGPKPSPEEGQAPVEAEPTAAPNLEDAIKDAV
                     LDTLFK"
     gene            40204..41856
                     /locus_tag="PL162_01975"
     CDS             40204..41856
                     /locus_tag="PL162_01975"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQF90829.1"
                     /translation="MPFLSRSYLRPLLLDIVASPSVSPSGDRENAVARRIVEHLADLP
                     YFQEHAEDLMLLPWEGDPLGRHLVFALVRAPEGSGDTVILTGHMDVVDVKVCGPLAPL
                     AFDAEAYTARIGEMPMDDDAARDLASGEWLFGRGVADMKCGVTGCIGVLAEASTRRET
                     LRTHVAVLFVPDEENSSGGMLAALPHLERLQRERGLRYLACIDTEPSIGMGDEALARV
                     YTGSAGKINPFFLVVGCEAHLGEYYEGLNSAAVLGELLRLAEGNPDLADEGGGEAFTP
                     LACMKHRDLRGEYSASIPERSVAYFSYFTASKNPCQVLDLLRSLALEAQRNALDRQEE
                     ASARFAARGKPRVHPRPREARVMTFEELLSKARDRVGPEADTLVERAAREAPGDERDR
                     ALAAADACVTLAGEKGPLVVVGFLMPWYPHRGNRGETVGDRAMLRLASRVAAEARDRF
                     GVALEIRPFYEGISDLSYCGYSEDPEGMEAYIRNVPAYGVDYRLPVQELQTLHIPVLN
                     LGPIGKDAHKHTERIHEGYAFDVFPRMLRRAVELVPAMYGGE"
     gene            complement(41961..42695)
                     /locus_tag="PL162_01980"
     CDS             complement(41961..42695)
                     /locus_tag="PL162_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90830.1"
                     /translation="MDPRVSWASYDQGDFAGAHAGFLKAQCGSPEKPTYLLVAGIAAS
                     RTSTVEYPQGPFLGILSSYGDATNFQTGAPLKPLAFAYAARLAAEKGLSLDVGAAVAS
                     YGDGYESDEVEWLDEAFPRRSDASGDEEQREFRSVGRPAPVRGVGSYYWAWALAQSPS
                     SADRMEALGILEREMPHFTGDLVERGVPRDLRREGERLLEALSNTPAGDSGPWGLYLI
                     AGILFVGELFLVRHLRRKRRGNPPPA"
     gene            complement(42885..43442)
                     /locus_tag="PL162_01985"
     CDS             complement(42885..43442)
                     /locus_tag="PL162_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HQF90831.1"
                     /translation="MLTAAILGGVLLVAALWGVGTYNGLVRGKNLVAEAWSGIDVQLK
                     RRHDLIPNLVETVKGYAGHEKQIFEDVAKFRNAAIGAGNPRDAAVAEMGLSGALKNLF
                     AVAEAYPDLKANTNFLQLQQELGNTENEIQSARRYYNGAAREMNIRVESFPSNIVAGM
                     CHFSKAEYFEMENPAERETPSVKFS"
     gene            complement(43633..44910)
                     /gene="tyrS"
                     /locus_tag="PL162_01990"
     CDS             complement(43633..44910)
                     /gene="tyrS"
                     /locus_tag="PL162_01990"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HQF90832.1"
                     /translation="MGQNVVKFFEERGLLEWCSAPEELAQVFERETVTAYIGFDPTAD
                     SLHVGHLIPIMGLAWLQRLGHRPIAIAGAGTGLIGDPSGKSKERNLITLEQMEKNLQG
                     VHPQLAHFLNFDCGENSARIINNYDWLGKLPLLDFLRDIGKYFPMNYLVNKEYVRSRL
                     DDPEKSISFTEFTYTLLQAYDFLHLFEGEGCKLQMGGNDQQGNIVSGIDLIRKKTGGE
                     AFGITYPLLLTSSGQKFGKTEGGAVWLDPKRTSPYKFYQFWVNVEDRDVERLLRLFTF
                     LPLEEIREIAARHAAAPERREAQKTLAEEITRTVHGEKALAGAVRATEILFGGKFDLE
                     DLTPEMLATLEAEVPGGEAPREERAVVDLLVASGACASKGEAKRLIQGGGVSLNGRKV
                     ERDDQSVVPEDILFGGHLFFRLGKRRFHMVRLR"
     gene            complement(45179..47338)
                     /locus_tag="PL162_01995"
     CDS             complement(45179..47338)
                     /locus_tag="PL162_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQF90833.1"
                     /translation="MWWSSWRIRTKILVGVLGVVVGVLGVIVGYVSWGSRQLATEDAF
                     DKAQALAEGYAHRVGREFNEAMDTARALSATLAAMKGSEHPSRELALEALRRVLETNP
                     RFLGVWTVWEPNAFDGKDAEYVGKPGHDATGRFVGYWNKGSGSVMLEPCVEYANTGAS
                     GEYYFKPLRSGEEVLMEPFTYEVGGRQIQLVSHCVPIVVEGKTLGVVGVDMSLETVQQ
                     IIASEKPFGGKGSLTLLSFNGVIAGHPKAEILGKTVRDVSRGQADTIIGAIQKGQVLA
                     LRDYSAALNGDVYKVHVPMAAGHTKTPWSLVVMAPFSVILEGANRMTLLASLVGLAGI
                     VLVAVVVMLVAGSIARPVRRFAEHIARFADGDLTVRFEARGEDEVAQMARALDSMAQG
                     LRRSMAAIREKAETAAVQADALASLSQETVASMEEVRSSVAEVATQSEANAAALEQTN
                     AGVEEVSSGATSSAHSASEGAETALRTTKRSETAGSRVMQVVDDVKGVGVKARVTRET
                     INQVATSVGTITKFVATIRNIADQTNLLALNAAIEAARAGEAGRGFSVVAEEVRKLAE
                     ESNVAAKEVEAIITGLQQQARNALDATDEGGRIAEATVKGAEEAGREIAEVLREIARI
                     NDAMQNIAAASEEQAAASGEMASAVDSATQGTSHIVETLEVIRTATDETTKASEEVAR
                     SARELSAGSKDICDELAHFRVHTQDAKPAPKPLALKR"
     gene            complement(47438..49756)
                     /locus_tag="PL162_02000"
     CDS             complement(47438..49756)
                     /locus_tag="PL162_02000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HQF90834.1"
                     /translation="MKTTLLRSTFSLCLLLVLACPAAWGVPACPYPIPLVQPDGTSGV
                     VFHRGDEFFHWTEAPSGHTLVRNAATGYWEYAALRDNSLVPSGTVYSPFGPPPAGTAP
                     HMIPAPRRLGAPDFDKKFGYPPTRPVSGKRNVLVVLVSFRNQALTTKTGDWEGAFFGA
                     TASLAKYYKDQSKGKLEIISARGGTAVLSVTLAAGDVNDGNHPQGFIDIGGDDNVDEQ
                     HKNEVAFVTAVAQKATALGVDFASFDTNKDGALTPDELCLYMIIAGYEQSGSKLKPSV
                     WAHAWKSWMNESDEHIVFAGGKKLTSWSMNGELYAKGTRMPFGVVAHELGHQLCALPD
                     LYDTSKTNSGIGAFSIMAGGSWGKKAKETSGSTPTNLDAWSRLYLGWATATTPASGVA
                     TFGEPGNSKYDVVKLVGTSHRKTEYFLLEVRRPSGWDQGLEGLEDSFADFDGGVLILH
                     LDEEVGTPSANDFNKYVKGKHQGNMVKEADGPHLTKDAESDGTKDTLWYQGNPNYEGD
                     GTFSATSSPNSKFHDGTASGFSLREFSAPGAVMTAKISAPGPTPTPIPAPTDMPEPDP
                     EVTGGVPLTSPTPVPPEELPPVGELNPSNVRFAPEEIAEIFGDDAEATFVLVDIGSAD
                     IVEYATPDGTLTLALASETLQEDDVIYRWIFFVRDRVSQVWDMPENAWDRQDTPPGYA
                     TLTCDIEDGGSFDLDGVVDGSVVVGTLEALVGVPGADSEPTGVSAGHRGGCDGGIVPL
                     GALLLLAPLAAMGRRRGQAPERPSDRTSRKNH"
     gene            complement(49914..50798)
                     /locus_tag="PL162_02005"
     CDS             complement(49914..50798)
                     /locus_tag="PL162_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQF90835.1"
                     /translation="MELVPCGGSTYYIPGSTNVGVVVREGRAVLIDSGNDESLGRKIF
                     QLLERQGWTLELIAHTHSNADHIGGNALLQKRTGCRIAATPREAALIDDPALEPLLLW
                     GATPFREICSKFLQASPSRVTDRFRAGDPVGESGLLSFPLPGHFLDMAGFRTPDGVAF
                     VGDSVFAEEILEKHGFAVCCDVAGQLQTFRQLEAMEAEFFVPSHATPSKDLKELVRYN
                     RHRTERLSEEIALLCAIPRSREEVLEALAEAYDIEITPVSFVLTHLTVAAHLSWLANQ
                     GVLRPRGERGRLLWERTA"
     gene            51094..>51924
                     /locus_tag="PL162_02010"
     CDS             51094..>51924
                     /locus_tag="PL162_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90836.1"
                     /translation="MDKSSSGDERAPITSPLAYLAAHYTTSDDLALQKQLSEDLARFS
                     SLPEDSADPEVRKERRAIWERASSSYWILAWEVLESRSKGSGRAPEFLDFAPEERLLL
                     DAAVLDPRLAPFNGEDLLASAEVPTPLDRYQYYRFSDLMAEAFALLFEKDLPRPRGGM
                     GLDEKIAHLERRLEGLTRRRGGMQKALLRHVSFGGLEEAEKDLREMEKRLLDQVEVDQ
                     RVQRFRFAEDAAQIRMRESKALYDRTRQHLIDLLEPLRDGLGAMGFLGEFDQFLALTP
                     E"
BASE COUNT         8380 a        16356 c        17729 g         9459 t
ORIGIN      
        1 ctaaaaggcc accgatgtta agccgttccc ggacagacag agggagcggg aacactcacg
       61 ctctccatcg aggccgcgaa gttgcttggt gtgcgtgcgg atctgatgtc gccagaaaag
      121 aaacgtgtcg gtcgaattct tgcgcgctcc acgctgaaga cttccgcgaa aagattccat
      181 gcgaaatcca cgggagtcta cgagatccat gtatgggtga agaataaagt actccggacg
      241 ggagcgtacc gcgtcggagt cgacgtccga taatctcctt cggggcaggt atccgcgtcg
      301 cacaaacaag ggggcggaga gagtgtcgtc tccatgtctc cttctcccga ggtggcgatg
      361 gaggcatgag aaacatgaag gatatgaagg agctctttga aatggcgttg gggttgggcg
      421 ccgagtggca cgtgaccggc atcacgctct cgccggagga gcatcggatc gacatcgccc
      481 tcgacttcga ccggggcacg aaattcccct gctataagtc gacggttttt ttgtcttgac
      541 aatgggggcc accataagaa caaggaggat gatgtgtatg aaacagactg tagaagctat
      601 tatcgacgag cgcggtcatg ttcgtctgac cgaaccgatt ttcataaccg gaattcgtcg
      661 cgcactgctg accgtgctcg acgaaccgcc ctcggccctc gacgagacgc tgctttcggc
      721 ggagcaaagc ctggctgcgg attggctcag accggaggaa gacgacgcat gggcgcattt
      781 acagtaggct cggtggttct ggtaccgttc cccttttccg atctttccgg ctccaagaaa
      841 cgcccggcac tccttctcgc ggatgtcggg cgcggcgact gggtctgcct gcagatcaca
      901 agcaacgcct acggggattg tacagcgatt cttctgaagg acgaggagtg cgcagaaggc
      961 tttcttgcga ggacgagtac catacgtccg gggaaactgt tcactgccca cgaaagcctt
     1021 tttatacgct gcgtcgcccg tatcgcggat agcaagatgg atgaagtgcg gcaaacggtg
     1081 cttactctta tcaagaacgg ccgttttcaa gaacacatct cttgatcccg tcgctcgaac
     1141 ccttggcgct tgccggcgcc ccgggctttg caaaaaaagc cggaactccc gagggagtcc
     1201 cggctttggg gcaggatgcc cgcgttttcg tgcttagtgc ttgggttctg cgtcggtgtc
     1261 gccgaagccc accttttcca gccaccaggt gggataaccc ggggtgctga aggagccgtc
     1321 gggggagatc accataccgt cgtgatactt gcccacgagg tgccaagcga ggttccacca
     1381 cgtgtcgagc ccttcggtga ggttctcgtt gacgaagcgg ttgacgaagt ccacggccag
     1441 cttgggatcc ttctggtagt actccagggc cttcttatcc gcctggggca gctccaggaa
     1501 gtacttgccc tcgagctgct gctgcacctg ccggatgtct tggatcatgt ggctgaactt
     1561 cagatccgcc cagttcccca cgaagttcca cgcccaccag gcgctgtccc ggtcgaactc
     1621 gtgccgtttg cccacggcgt aggggcgggg gacctccgtg gtgcccgcgt agatgggcat
     1681 gtagagggtg gtggagggct ggtcctcgcc gaaccagagc acggcggcga tggggtcggg
     1741 catatcgggg cgcacctggc tcacgaagct gtaggagcag cggaagaggg agatggagcg
     1801 atcccagtcg ttgcccttac gattttcggg gcgcacgccc ttgggggtgg cgtaccgggt
     1861 gggggtgccg aaggggcccg ccgccagacc ggtggtgagg tcataggggg tgccttcgag
     1921 gttgtcgcga tagaccgcca tgaggtcctg cacggagagc ttcttcgagg gcttgatcac
     1981 cagggggtac tgctccgcca ggttgggatc gagcttcagg ctgggttcga tcagggtgta
     2041 ggcccgccac tcccggcgct gctggtagta gggcgatccg taggggttgg ggttgtagat
     2101 cttgtggaag acgaagggtt cgccatcttt ccaccatccc atatcctggg cgaagctccg
     2161 gatgttggag gagtacatga aatcgttttt gttatcccac tcgatctcgg cgatgcgggc
     2221 ccggttggcc cccatggcaa agccgtcgtc gggaatgcgc tgggccaccc aaatggcccc
     2281 gggtttgccg ctctccgggg tccagagggg ccccgggccg cagatttcaa agagccagac
     2341 ttcaccgttt ttgtccgcca cggagaccgc ttctccgccg tcggcgtagc catacttttc
     2401 cgccagttcg cccatgatgc ggatggcatc ccgggcggtg gcggcgcgct ggagggccag
     2461 ggcctggagc tgctcgatca tcatccagcc cttttcctcg cacaggtttt catcgcgccc
     2521 cccccaggtg tactccccca tgacgacgga tttctcgttc atgaaggggt atcccacgtg
     2581 gaagtaggta taggtctccg gcacctgggg gatttctccc accagctgga gctccgcctt
     2641 ggggcgggtc tggtagcaga tgtttttgtg gatgggaacc atctcccccg ccttgtgggg
     2701 gcctccgggg atgatctgga cccgctggtc gtaccacccg tcgcaggtat ggctggtcat
     2761 gaccgagccg tccacggtgg cgtttttccc caccatgaac acggtgcaag cccaggcgga
     2821 tcccgcgctc accgtcacca gagttgccac cgccagggcc cttagccagg atcgatactt
     2881 catctcatgt gtcctccctt cgacgtgctc gtttccaaaa aagacgcctc ttaccgctcc
     2941 gcgctccgat gcattcttcg tcggggtccc acctccttag tcatcgctcc tgctctgctt
     3001 gggcgcttct tggcccctcg tctgccctct tggggaaggt cagtcgtagt aaacccggtc
     3061 cgccgaggcg gcggaggggt tttgcagaaa gacgccggtg gtcttttcga tgacttcggc
     3121 gtaccggggc accccggaaa gcagcagggg tttgtccggc gcggtcatgt tgtacgagtc
     3181 gatggtctgg agcagggcgg aaaggtgtcg ccccacccgg acgtcgatgg gccacccctg
     3241 gtcgtccatg ggggcgtaga ggagcttgtg ctctccggcc tttaccacga agtcatcccg
     3301 cccctccatg aggagcttct cgtcggtgat gccccggatg cggtccagca tgggttccgc
     3361 cacctcgaag atcatggaca tggcctcgga gtagtccccc acctcccggt gcgacaggcc
     3421 gtgcagggcc ttgggagaga attccatccc catggggacg gggaactcct gggcggtgag
     3481 catcatggag gtcatggccg ccaccgcttg cccccgttcg tgggtgacga tggtgttttc
     3541 cacggggtat tccagctccg cctcgtggta gtcgatgaag atgtccactt tttcctggcg
     3601 caccacttcc atgaaggcgt agttggtgcg ttcggtgatg agcccgttgg cgcgcccggg
     3661 ccaggtgcgg ttgaagttcc ggatgtccat gtaggccagc atctgtcggg agggatagtg
     3721 cagatacacc tcggggtcgg gccaggaatc caacgcattg gagcaccggt ctcccatgcg
     3781 gtaccgggcc acgccccagg gggttttgac atggaagtag gtggggtagg cctcgccggg
     3841 gcgggtgcag gtggacgcgc tcctgttgac ccgggggacc accacgagct tcccctggtc
     3901 gaggtgggcg ttctgaacca gcaccgccgc cgccagcacc cctgcgggtt cttcggggtg
     3961 ggtgcccccg atgaccatga cggtggcgcc gggtttgccg ctgtccagga tgtagacgtg
     4021 gcagtcattg atggtgtctc gtactgcggg ggaataggaa gagaggaggc gcacctcggt
     4081 gacgccgggg cccgccacca tgggctccag gtagtttcgg tgctccagaa attcttttcc
     4141 cgccacgaag gcgcagaggc ccgccaccgc tagggtaagg agttttatca ggatatttct
     4201 agattccatg gcctcgcccc cctattttac gcgcaggagg cgcaacacga agggcacgag
     4261 gacgatggcg tagagcaccg catcctgaac gttccgagtt ttcacgaagt tccaggcgat
     4321 gacgaggccg aagatccccg tgagggcata gtagagcaac gtgacgatct cattcatctg
     4381 gaccagcatg gctttagcct ccgaggacat cagcaatttg cttgctgtag atcaggaaga
     4441 gggtgcccag gacggaaatg aacgccatgg ggaccacaca gcttttgacg aactgcccgt
     4501 agtaatctcc ctgatagccc agctccatca ccgccgcccg gcccaccacg gcggtggggg
     4561 ggaggcaatc tcccaggggc cacatgacgg ccatggcgga aagggccacc acgggatcga
     4621 gccccttcat gttgaagagc atgacgaggg gcacgccgat gaggggcgcc acggcgtact
     4681 gcaacatccc ttcggagatg gggaggatga ggaaaagggt ggcgaagagc gccgacaggg
     4741 gtagggtcac cacggaaagg gagatgagcc cccgggcgcc gctccacgcc atgatctgga
     4801 tgagggatcc caccaccacc atgatgccca ccaggggcag gagggactgg atcgtctccg
     4861 aggcgatgcg ccccagggga agacgccggg ggctcaggac cacggtcacc agggcgcaga
     4921 gggtgaaaat gagggggagc cccagcacgg gcatgtggaa cggcaggagg cgccccgctc
     4981 ccaccagaag ggccaggagg ccaaagggaa agagcacccg gccccaggtc atgccttcgg
     5041 gaacttcggg gagggccccc agggcggtgt cccggtcgat ggccttggtc cctccggcga
     5101 gccagaacat ggaaaagagc gctccggtca ccgaaaggat cgccagaggg gcggcgaatc
     5161 ccacgtaggg catgttggcg ccggcggcgg ccatcatggc ccagaggttg atgggggggg
     5221 cggcggcgct catggcggcg cagagaaaga tgatggccac ccggcgggtt tcttccaccc
     5281 ccatggcggc cagcacggac cccaccaggg cccccacagt gaggacgctc gccgctccgg
     5341 agcccgtgag ggcgccgggg atgagcatca ccagggtgag gagcaacagg cacaaaaatc
     5401 tgcgctcggc gaaggtcccc acgatctttc tgacgatgaa ggccacgccc ccggtctctt
     5461 tcaccagcgc catgaagaac gtggcggtga tgaagatgag gcagacgtcg aagtaggtga
     5521 aggtcccttc caccaaatgg cgcaccagcg acgacagatc gccgttcatc acgccgtgga
     5581 cgcacccccc cgccacggcg gcgaggagca tggaaagctc cgtgctcacc tggaacttct
     5641 tggcggcgag aaaggccccg acgatgacgg caagcaccaa cgaagcatgg acaaacatgg
     5701 gaaaacttcc tttctgttta tgcgacgccg cccggaaagg ggcgggggtt tccggtctcc
     5761 tctttccggg cggtgtggat cgatggggcc tacttgttgt atgccgccag ggtggcgccg
     5821 acgcccaggg cgtccttggc caccagcagg ggcttgttca gttccttggc gtacgtcgtg
     5881 aagaacccgt ccttgtcgct gtcggccacg atgagaatca tgtccgccag gggcatcacg
     5941 gccttgatgg acgcttcgtc ggaggcgtcg gtgcggcggg ccatgccctc gatgtgggcg
     6001 ccgatgacca tcatgccttc ggctcgggcg gcctcgatga gagccctgac tcggtcaatc
     6061 tctttgtcca catcggtgcc cgcagccccc atgcccttga ggctggtgcc ggtggtgacc
     6121 atgagggtct tgtacccttt gcccttgatc ccctcggcgg agagctggtt gtcggactcc
     6181 gtcggcgata gcttggcctg cagacaggac atcttggcca tgacggcccc cggactctgg
     6241 ccgcaggtgg tgatcaccag ggggggttcg acggcggccc aagcccccgc cgccgccaga
     6301 agcacggccg cgatcataaa gaaaccccaa actgcttttt tcatgacaaa acgcgcctcc
     6361 ttcggagaat atgttattac tttccatgcg aagcatagcc cccaaagcct cttttgtcat
     6421 taaggcgccg tcaaagaaga gcctttttgc cggggctttg ctccgccatt tatcccagga
     6481 tagcacagtt ttcccttcga ggaggtgatg tttcacgcct ccggggccaa tctgttacac
     6541 tgcgggggcg acaaaagagg ggagggagcg ggatgggtca tcgggtgctg gtgacgggca
     6601 cccggggcaa gagcggcatg gtgcgtttgc tggacgcggc cctgcgggcc tgtggcgtcc
     6661 ggtgctggag tcggatcacc ggtgtggtgc ccacggtgta ctcccccgaa ggggcgtttc
     6721 gcctccggcg caccgccccc gcctcggtgg aagagatgcg ctggtggctc gctacccttc
     6781 cccggaacgc ggaggcggtg gtgctggaaa acagtgctgt ggcgccggaa ttgcagcccc
     6841 tggcggggcg ttggctccgc cccacgctga cggtgtggac caacgcccat cccgatcatc
     6901 aggaggtctg gggggcgggg agcgaggacg ccctccgggt gcttttccag gggattcctg
     6961 cgggcgttcc cgtggtgctg ggtcgggagc tgggggcctt gccggaactt cttcgggcgt
     7021 tggagcgcca ggggtgtgcc gtttctgtcg ccccaggatc ggaggaggat ggggtcctgg
     7081 agcgccaacg ggccacggcc ctggcggcct gcgccgtgct gggactggac cgggggcgtt
     7141 gcctcgaagg catgggggcc ctggcccccg acccggggga tttccggacc ctgcgccgag
     7201 gggacggcgt cctggcgtcg gcctttgccg ccaacgagcc caccagcagc gaggggcttt
     7261 tcgcgtctct ccggtggcgg cgggaggaca cggtgctctg gtaccaccac cggcgggacc
     7321 ggagcggccg tctggaagcc tttctcccct ggtttcggcg ggggtggaag gaaatctggc
     7381 tcacgggggc cctgccctgg cgccccgtgg cgggggcgcg gattctggga aatcccccgc
     7441 cggaggagat ggagcggcgc ctgggggagg ggcttcggat cttcggctgc ggcaatgtgg
     7501 ccgggcttcc cctggccctc ctgacccggt ggaagggagg ttttccggca tgacggaaga
     7561 gatgggactc tttttcctcg cccttcttct ggcggggatc tacagccacc gcaccggatg
     7621 gggcacgggg ggcgtggtga ccccgggatt tctggcgctg cacgccgctc gtcccctgtt
     7681 tctggcggga atgctggggt tgggggtgct cctggccctt atgctggagg tgttggtgcg
     7741 gcgcttttcc ctctttggcc gggaaaggct caccgtggcc ctggtgcttt ccgtgggggc
     7801 gctggggacc ctgggggccc ttttcgccgt gccccttccc tggtacggct gggtcattcc
     7861 cggactggtg gcggcggact gtcaacgcca gggggccctg gggaccctgg ggggcgtcct
     7921 ggcctgcggc ggagcggccc tctgcgcccg gggcctgctg ccatgaggcc ctccgcgacg
     7981 gacttttcct ggcgctcgct ctggcgcggg tgggggccgg ggttttttcg gtggtggatc
     8041 tgcggcgagc ctccctttcc cctctgtctg gcccttctgg ccctggggct tgccctgttg
     8101 gtctgggggc cctggtccgg ccgtgcgggg ggcattgagg gcaaggccct aggcaccgtg
     8161 cggcgggccc aggactatgt ggtggcgtgg cgcacggagc ggggcatccc cccttctccg
     8221 gagagcgatc cgtggcacac cggtctggtg ggggaggaaa tgacggccct caccaccacc
     8281 ctgggcgatg tggccgccaa acgcaccgcc tgcgaccccc ggtgggtgcc ggtgctcttc
     8341 cggtggttcc ggaaggcggg gttgacgtcg ggggaccgga tcaccgtgtt gccctcgggg
     8401 tcgtttcccg gatttcttct tgccaccctg gtggcggggg aggcggcggg gctccggatc
     8461 cagcttttgc cctctctggg gtcttcctcc tggggggcca atcggggcga tctcactctg
     8521 ctggatcttt tggcgctctg gcgccggggg ggcttcgtga ccacccgggt cgaggcgtgc
     8581 accttgggag gcggcgagga aaacgcccgg gacatgcccc aggagggggt ggagctcctc
     8641 cgggaagccg cccggcgcca tggagttccc gtgctggaag cggaaagctg gcgcgccctc
     8701 cgggacctca aggccgcccg catggaggcc ttttcccccc ggttggtggt gcagatcggc
     8761 ggggggatga cgagcctggg agatgatccc gcggtgctgg aggtccctcc ggggctcctc
     8821 ctccccgaag agcacgcccc ccggggcgat gggctcctgg cctgggctct ggagcgccgc
     8881 attcccgccg tgcaccttct ggacgtgcgg accctggccc gccgggaggg ggtccccttt
     8941 gacgcccccc ccgatgccgc tttcgggagg atggctccag ggcccgccct ggtaggactg
     9001 ttgctttttg ccctggtgct gggaacccac cgccggtgga gcctggagcc cctggagacg
     9061 ttccggagcc ccggagattg atcccccgga aataaccgga tcactctctc gctgtagggg
     9121 cgagcatatt tttcgcccct acgtaactcc gagattttct agctccccac ccagcgcgaa
     9181 aactttcact cctcggctcc ggagtcggga agatctctgg gctgaacatg gcccctgcgg
     9241 cggagtgcgg gggaggaacg gtgtgttttt tggcaaaagc gagccgaagc ggcgaaaatt
     9301 cacgcatcct ttcgcccggg ccgaaggaga tctgcgccat cccccggaaa taaccggatc
     9361 actctctcgc tgtaggggcg agcatatttt tcgcccctac ggaactccga gattttctcc
     9421 ccctgctgcc gaaatcggga ctcatctttc cgtcccgtgc cggatgaagg ggcttgacga
     9481 gggagaacaa aactggtatt ttgataccaa tataaaaaat aaccggcggt cctccggaaa
     9541 aagtcttttc cgcaagggat ttcgcccgta gaggaggaaa acacatgaag gttcggcggc
     9601 agatgatttc catcgacgag gagaagtgca acgggtgcgg gctctgcgcc cgggcgtgtc
     9661 acgagggagc cctccgcatc gtggatggca aggctcgctt ggtgagcgat atctactgcg
     9721 atggtctggg gaactgtatc ggtgcctgtc cccaggacgc catcaccttc gaagagcggg
     9781 aggcggaggc ctacgacgag gaagcggtgc ggcgtcatct ggcgggggaa acccatcagg
     9841 cgccggaggt aacgcctcct ccccttccct gcggctgccc cggcacggcg gcccggagcc
     9901 tgcgggatgg ggcgaaggcc ccgaagaccg agggggaggt gccgaagggc ggagctcctt
     9961 ctctgttgga gaactggccc gtgcagcttc gcctggtgcc cgaaggggcc ccctatcttc
    10021 agggcgcccg gtgggtgctg gcggcggatt gtgttccctt tgccctgggg gactttcacg
    10081 cccgctttct ggcggaggag gggacggtct gtctcgtggg gtgtccgaag ctcgacgacg
    10141 ccggggcgta ccgggacaaa ctcgcccgca tgatcgccgt gtcccatccc cgggagatcg
    10201 tggtggtgcg catggaggtg ccctgctgcg gagggttgtc ccggttgttg gaggacgcgg
    10261 tggaggaggc ccgggtggat cttcccgtca ccacggtggt ggtgggactc cggggcgatg
    10321 tgggccccgg ggaggtcacc cggtaccgct tccggcagta agaaaaggtc accccaaggg
    10381 gggaggctcg cgacgtctcc cggaaaagac cgggggcctc tgcgccgtgg gggcgaaagt
    10441 agctctcgga ggcgtcgatg ttctgcgatg cggggggcac ctcggcccac gccggtgacg
    10501 catggcaaaa agcgtagggg cgcccagaat atgatgtaca cctcaaaagg ctaaagagag
    10561 ccggctggaa ggtgagcgct cccggctcgc gcgtccgggg ggttcccggc ggggtaaaag
    10621 gtgacgcagg gcaaaaagcg taggggcgag catattgctc gcccctacat tgcccgctgc
    10681 ggatatctcc gccatggatg ctctccagtc acggcctccc cgacgtgccc ttactccgcg
    10741 cccttcgcag agcgtagggg cgaaagccta aaagttgatg tacacctcaa aaggctaaag
    10801 agagccggct ggaaggtgag cgctcccggc tcgcgcgtcc ggggggttcc cggcggggta
    10861 aaaggtgacg cagggcaaaa agcgtagggg cgagcatatt gctcgcccct acattgcccg
    10921 ctgcggatat ctccgccatg gatgctctcc agtcacggcc tccccgacgt gcccttactc
    10981 cgcgcccttc gcagagcgtt catcctgcca ggctccggtt ggtaccgacc atgagcgcgc
    11041 agacgggagc tcactgcggg gagcgcacat cttccagccg gcatcaattc gtttcgcgtg
    11101 gccgggagcg ccggcttcca gccggcatcg gttatttttt tttacaaaaa ccccccatgg
    11161 gggctcgcat ccttgtcctc atcagggaac accgatctcc agcacaccag tagggccgta
    11221 gggggatccc ctccggccct cctcggccca cgttggtgat gcggggcaaa agccgtaggg
    11281 gcgcccagaa tatgatgtac acctcaaaag gctgaagaga gccggctgga aggtgagcgc
    11341 tcccggctcg cgcgtccggt ggcgggggga tcgttgttcc gatggtcagt aggtccactc
    11401 gaaggagccc cctccggccc gttcgatgcg ggcctggagg tctttggcgt tgcgctccag
    11461 gtcctcggga gttttccctt cggcccgggc cacgaggatg ggctgggtgt tcgaggcccg
    11521 taggagcccc caccccgtgg ggtagatgat gcgcactccg tccacggtga cggcgtcgaa
    11581 atcccggagg gcttcctcct tgacgcgctc caccacggcg aacttccggt cgtccggaca
    11641 ggggaaacgg gtttcggcgg tgctgaagta gcggggcagc gaggccacca gggcggagag
    11701 gggctccggg gagtgagaga ggaggcgcaa gaggcgcccc gcggcgtaga aggagtcgtc
    11761 gaagccgaag aactcgtcgg cgaagaacat gtgtcccgag acctctcctg aaaagagggc
    11821 cccgatctcc ttcatccggg ccttcaccag ggagtggccg gacttccaga actggggctt
    11881 gccccccagg cgcaccacct cgtccaccag gagctgggag cttttcacct cgatgatggc
    11941 gtcggcgccg gggtgtttgg ggagaatttc ccgccagtac agggccatga gttgatctcc
    12001 ccagagcact tccccctggt cgtccaccac gccgaggcga tccgcgtcgc cgtcgaagcc
    12061 caggcccacg tcggcgtggt ggcgccgcac ggcctcgatg agggggggaa ggttctcccg
    12121 cactgtgggg tccggatggt ggttgggaaa ggtgccgtcg ggctccgcga agagggggat
    12181 cacccggcac cccaggcgcc cgaagaactt ttccataaag agccccgcgg tgccgttgcc
    12241 cccgtccagg accaccgtga gggggcgggg gcccagggaa attttttcca ggagcatctc
    12301 cacgtaggcg tcgccgatgt gggcctgctc cagggtgccc cgggccgcgg gggtggcgaa
    12361 ggcgtcggcc tcaatgatgc gccggatttc ctgaatctga tcgccgtaga gggtgagctt
    12421 gccgaaggcg agcttgaggc cgttgaattc cttggggttg tggctccccg tcaccatgat
    12481 gcctccgtcg caggccagat ggtagaggct ccagtagaag gcgggggtgg tgagatatcc
    12541 caggtcgacg acgtcgatgc ccgcgtcccg gagcccttcc agggcggcgg cccggatgcg
    12601 gggggtggaa agccgcacgt cccccgccac gggcacccgg cggatccccg atgcggcgag
    12661 aaaggtgccg tatccccgcc cgatgagtcg cacggtggcg tcggtgagct cttcgtccgc
    12721 tagcccccga atgtcgtact cccgaaagat gtgtgccggc accacggcca atgtgatcgc
    12781 cctccttttt tggccccttt agggggcgcc ttcccgagag ggccgggcct tcccggggtt
    12841 acccccgtat tctactacag ctcctcggtg gggggagttt tttcgccgat tttcacgccc
    12901 tctgggtggg gcggggaaag atatgctata gtggcccccg gcggtccctc gccgggcgcg
    12961 gcctgatggc gctccgggtg gcgggggaaa gatgatcctg gggggacaaa atgaacggcc
    13021 tgcgcatggt ttctccgttg gtgatcgtca tggcgctggg gtattttctt cgtcgtcggg
    13081 gaatgctgga gggggacgtg acgttccgga tgaaccgcct gctcttttac gtggcgcttc
    13141 cggcatggct ctttcgatcc atcctccggg cgggggcggc ccccatggat catctgcacc
    13201 tcttcaccgc ctgtcatgtg ggttttttcc tggtgcctgc cctggcctgg ctcctggccc
    13261 tcccctttcg ggagccccgg gagcgcctgg gggtgtcggt gctttccgcc gttcggtcga
    13321 ataatatctt catgggggtg cccgccatcg gcctggccct gggagagccg gggctggagg
    13381 ccctgtcgct tttcctgggg ttgagcttcg tggggtacca catgatctcc atcggatggg
    13441 cccagctcgc cctctcgggg gagctcaccc tccgagccct gaaggagatg ggtcgggggg
    13501 tgatccgcaa tcccctggtg caggcctctg cggcgggcat gggactctcc ctcctggggg
    13561 tgaaggccct gccccaggag ctggacgtga cgctgaagat cgtgggggac gtggccagcg
    13621 gcctggccct gatcaccctg ggggcctcgc tgcggccgga gaggttctgg cgggggttgc
    13681 gccactcctg gagggatgtc tcggtgaagc tggtgctcca ccccctggcg gtgtgggccc
    13741 tctttcgcgt ctggcccgtg gcgcccctca tggagcaggt ggtggtgatc gtggcggcca
    13801 tgcccgtggc ggtgaacacc tttgtggtgg ccgagggcat ggggatggat gccccctata
    13861 cggcggagct catcgcggta tccaccgtcc tgtcggtggg gacccttccc ctgtggatgg
    13921 gcgtcctggg gatgggatag ggacggaagg ggcaaggggg cttcccccgc ccaaggcgaa
    13981 aaacgaaagg gaggttcgtc atggatgcgg tcctggtctt tctcctggcg ggggacatca
    14041 tcgccaatgc gggggcccac gtgctgctca aacagggggc ccaaaagccc cgatcccagg
    14101 cgccggggct ccggggcatg gtggaaaaca tcctgttcaa tccctgctcg ttggtgggca
    14161 tgggggccta tgtcctctcc ttcgtctttt acgcggccct tctgacccgc atggatctct
    14221 cggtggccta tcccctctgc acgagcctgt cctttctcat ggtgcttctg gcgtcggtgc
    14281 tctggttccg ggagcgcctg gacggggtgc gcctgggggg catcggcgcc attttgctgg
    14341 gcatcgtggt gatctccatc gggggatagg gcccggtcgg tcgtggtccc agcgccgcaa
    14401 agaagcggga ccccctcgga agaagggggt cccgctttct tgatggccat cccgttaatt
    14461 gatagaggtc agttggaagc cggtttgggc tcctccccct ggggcgctcc ccaggaaccc
    14521 ttcattttct cccactccgc ctcttgggcg ccgtcggggg cgaagaagtt caggtcgatg
    14581 gagccctttt ccaggccgga gcacttcacc accaggtcct gaaccgtggc gatgaaggcg
    14641 tgggcttgga cgatggcggc gccgacttcg tccatcccgt cctccaggat ggatctccag
    14701 atgtccgccc ctttgaggaa ggtgaggagc tccttctgca gagcttcgtt atcgatgtag
    14761 aggtagttgt agagcgaggc gtctccctgg agctcctgga gccgagggga tccttccgcc
    14821 ggagtggcga gctggttggc gtccaaaagg cccaccagca aggtggagcc ccgatgagct
    14881 atggtgaggg tgacgggcag gggcatgccg tacaccgcgt cccatccggg gatggtcacg
    14941 ggggccaggg gcatctcctg ggcctggagg gtcttcacca gaagatccgc cacggtcttg
    15001 gcgccgtcgc cctggccctc gaagaccaga taggccccgg gaagaggggc ggaggcaaag
    15061 gaggcggtgc caccgagcac ggcccccacg gagcccgtga ggaggctttt cagggtttcc
    15121 tcggagaggc ccatctcctg tacgccctgc aggaactctt cgaagccatc ccgcacctct
    15181 tcgtcggggt tgttttgcag cacgctcttg aggtgatcgt agtcttcgga gaaatggaag
    15241 gcccccagga ccttgccccc tccgaggagc ggcaggggag aggagaggct cttaaaggct
    15301 tccagggcct tgggggagaa gagggcccgg gccaggttgg tgaagattcc cagggaccac
    15361 tggttctcct tcacggcgca ggagaactcc atgagcacgt tgtcccgaag caggtagggg
    15421 acttcggcgg ccataagggc gatctggagc aggccgttgt cccggaacag gaagtagttc
    15481 ggggagtcgg ggaagtggcg ctggggggcg aagcgttggg cggggtcctt tagggccgcc
    15541 acggactcct gaaggtcttc ggggcggaag cccaggagga agaggttgcc ctgggcggcg
    15601 aaatgcaggg cgaagggggt cccttccagg gggactcgat aggccgtcgt cccgtcctcc
    15661 agggagaggg gaccttccac ggggaccccg aactgggtca aaagctccag gtcttcgctg
    15721 atcatggctc ccttggcgat gcggctcaag agatccgccc gggcgggccc tacggagacg
    15781 gccagttgga ggcgcaggac gctcttttgc atccccaccc cgaaggcaaa gccctccacg
    15841 gggtacatgg cgttcacggc actcaaggcc tggattccct cctgggagga aggatccagg
    15901 gccagcaagg gggattcgac gatgcttttt acgagttttt ggacgtcgga gacatttccc
    15961 gccatgtgga aggactcatc gctccagggg ccgatggcgt ccgccggttg gtagcgttcc
    16021 tcggccaggg cgcctccggc ccacagggcg gcgaccaaaa gcaataagac cgcgccgatg
    16081 gatctccttt tgattgaatt catattatac acctcccgtc tgattgtcct ccggaggggc
    16141 cttgggtcgt atccctctta tgccctgtcc ccgggggaac gggcctacta tagtaaaacc
    16201 ttcctgcttt ggcaacaaga agatgggtct cgttcggctt tcgtatccct ttttcggaaa
    16261 ggggggcctc cctccggagg agatggagta aaatggccgg ggacaacgaa aaaatccccc
    16321 gaagctttcg gggggcgcag gggaaaaggc cgccccggag cctcggcgga gggcggcgaa
    16381 aggggacctt gcgatgacgg gactttgggg ggggcgcggt ggagaggggg ggaatgggtg
    16441 caccaaatct ttgtcttttc cggcacgggc aacacgtggc acctggcgaa gcgcacgggg
    16501 cagctcctgg gggacgcgga gctcgtcttt ttgtccgggc tccggcacga cgccgcagtc
    16561 ctggcccagg gggacacggt ggggatcgcc tcgccggtct atgcgggggg gctgccatct
    16621 ctggtgaagt ccttcctcgc caaagtggtg ttgcgacggg gagcctggag ctatcttctc
    16681 ctgacccacg ggggtaggcc ctttggcgcg gggcgccagg gggcccgcat cctccgggaa
    16741 cagggggtgg ccctggacgc ggtcttttcc gtggccatgc cttccggttt tcctccccgt
    16801 ctggggggga tcttcgcccg gcgtccggag gggcggctgg cccgggagga ggagtcggcg
    16861 gcggcggtgg ccgaggcggt gagggagcgc cgggggggag ggatcgaggg ggccttttcc
    16921 tgggtcaccg atctttttca cgaggggctt ttggcccggc gtcggcgcct tcccttccgg
    16981 gcggaggggc attgcacggg gtgtgggacc tgtcgagagg tctgtccggt ggaaaatatc
    17041 gtcatggacg gtatgcaccg tccccggtgg cttggttctt gtgaggggtg cggggcctgc
    17101 tatgcttggt gccccgtcgg ggcgatccgg gtggggcgct ttccctgggg aggggagccc
    17161 taccatcacc ccggggtttc cctgggggag atgcgggggg gctttcgcaa aaagcccttc
    17221 cgggtgtccc ggcgggagga ggggcctttc ccgggggatg tcggggggat ataaggggct
    17281 cggggggcga tgttgctccg gaaagagaac tctccaggca tggaagggga ggggtcatga
    17341 tgcatcagat ctacgttttt tccagtacgg ggaacacgtt gcacgcggcc gagagggtgg
    17401 gagaggggct ggggggtgcc cgtctgatca acatggccct ggcccaggac aaggagacgg
    17461 aggcctcggg ggatagcgtg gggatatgct atccggtaca tgccttcggc gccccagcgg
    17521 tggtacaccg ctttatcgag ggcctttccg tagaccccga gagctgggtc tacctcatcg
    17581 tcaccagcgg cggggggccc ctgggggcgg cggcccaggg ggtcggcctt ctggcccaga
    17641 ggggcgtgag ggtgcggggg gggtttcacg tgaagatgcc cggaaattac tcgccgcttc
    17701 acgattctcc ttcggggggc aagctccggc gcatggtgga tcgcgccgac ggggtcctgg
    17761 aagggatcgt cgaagacctc cgaaatcgac gggaaaagag acctgggggg agctcgatgg
    17821 agtggctttc tcgctgggcc aacgggtcgg ccttggcttc cgtgggagcg aaggaccggt
    17881 ctttttacgc cacttcttcc tgtgtacggt gcggccgctg tgtggccttg tgtcccgtgg
    17941 ggaacgtgga gcagggggag gagggccttc cccggtggat ggggcggtgc acccagtgta
    18001 tggcttgctt tcactggtgt ccggtccatg cggtgcagtt taactccacc acatccctcc
    18061 ggcgccatcg ctatcatcat cccgccgtct ccccggagcg ctttctcgcc tggagtgggc
    18121 ggtaggatca aaggggggaa tcggccatgg atctggcgcg gaaggtggag atcctggggg
    18181 cggcggcccg gtacgacgcc tcctgcgcct cgagcggatc ttcccgccgg gggacggagg
    18241 gggggatggg ggcggcgtcg ttttacggcg tgtgccacag ttggagcgac gacgggcggt
    18301 gtatctccct gctcaagatc ctccagtcca acgcctgcgt ctacgactgc gcctactgcg
    18361 ccaaccgagc cagcgggaac gccccccggg ccaccctcac ccccgaggag ctggcggagc
    18421 tcacgataaa tttctaccga agaaactaca tcgaggggct ttttttaagc tccggggtgg
    18481 tgggaagtcc cgacgccacc atggagctca tgctccgcac cgtttctttg ctccggggga
    18541 cccatgcctt cgggggatac atccacgcca aggccatccc cggggcctct tcggagctgg
    18601 tgtcccgcct ggggcgcctg gcggaccgca tgagtgtcaa catcgagctc cccaccgagg
    18661 cggggctacg gcgcctggcc cccgccaaga cccgggaggg gattttgggc cccatgggat
    18721 ttctcgccga gcacatcaaa gaggcggagg aggaccgggc ccgatctccc cgggcccccc
    18781 tttttgtccc cgcagggcag agcacccaac tcatcgtggg ggccacggaa gacagcgatc
    18841 tccagatcct gcgcctcgcc caggggctct atcgccgcta cgccctgcgg cgggtgtact
    18901 actccgccta cgtccccgtg ggggcggacg accgtcttcc ccccatcgtg gcgccccccc
    18961 tccgccggga acaccggacc tatcaggcgg attggctcct gcggttctac ggtttttccg
    19021 ccgaagagct tctggacgag gcgcaccccc ggtacgaggg cgacctcgac cccaaggcgg
    19081 cctgggcgct gcgccacctg gatctcttcc ccgtggaggt caacaccgcc cccctggcga
    19141 tgctcctgcg ggtgcccggg gtgggggtga aatccgccaa gcgcatcgtg gcggcccggc
    19201 gggagcgccg cctggacgcg ctggccctcc ctcgcctcgg ggtggtgatg aagagggccc
    19261 ggtacttctt gatggccaac ggccggcact gtggcggggg aacgatggac cccgcgtttt
    19321 tgcgcccccg catcggcgat gggaaagaaa gcccctgggg gcgccagctc tccctctttg
    19381 ccgacgcggg ggaagccaag agggggccgg aggccctgtt ccctgcgatg gcttttcctg
    19441 atcggcgtga tccggtggcc gccggagggg cgtgacatgg ccctctgggc cttcgacggc
    19501 tcctttgagg ggttgctgtc cacctacgcc gagatttttc agcggcaaga gactcccgac
    19561 gatgtggtgg tacagggaga agggtcccag ggcacgctct tttctccggc cttcgttccc
    19621 tcggacgaaa agaaggcgga ggccttcgcc cgggcggtgc ggcgccgcct gggggaccgg
    19681 accttcgaca ccgtcatggt ggcttttctc tccgagaggc ctcgtatggc gtggcacctc
    19741 tggcgctacc tagacctggg gcgacgggag gggcccgcct tcttcctcca gaggggagat
    19801 ccccgggtga gctccgtgca cctggcgcgg cgggcggtcc tgagggaagt ccaccgcctc
    19861 tcgggattgg cgcggttcga gcccctccag ggggtgctct acgccccctt ggcgccccga
    19921 tacgacgtgc tggagctcct gggccctctt ttcatcccgc gtctttctgg cgagcgatgg
    19981 atgctgcacg acgtggggcg aggcaaggcg gtgtgcggcg aagggggcga gctccggggg
    20041 gtggaagccc cccggagggc cctgaaggtc gtttcggagg gggaaaggga ttaccggcgc
    20101 ctctggcagc gatacttcga gatcatcacg gtggaacatc gccgcaattc ccggctgcag
    20161 aagcacttca ttccggaacg atactggaag tttctcacgg aaaaggccct ggaacccgag
    20221 gctttttctc cggggcctcc ctgagggggg tccccctcgg gcgggcctca cccttcccgc
    20281 cggggctcca gaagcctccg agggggcggc gccggaggga ggggatcttc cgaaaggggg
    20341 gcggggtcgt cccggcgccc caggcgccct tccaagtcct ccacggatcg tcgcagggtc
    20401 accagctctc gggtgaggcg ggtgtggtat cgggcgacga ggatctgggt ctccctcagg
    20461 tcgcgccgca ggtcttggta aaggcttcgg ggatcgacgg ggagggggtc tttctccggg
    20521 ggcacgcccc ccggggccgg ggaggtctct tcccggggag ggatctggtc cttcgggggg
    20581 atggcctggc ccatctcccg tcggagggcc ttgccctcct ccaccaccag ggccatcagg
    20641 gcctccaggt cgttccgttg gtcggccacg agctcgtgga ggtggagctc ccggtgagat
    20701 cggtcctcca ggcgggcctc cagcgcgacg aaacgggata ggatcgtcat gtgttgcccg
    20761 gcggcctccc gctggaggtc gcccaggcgg tgggagatct gcttggcgtg ggagtggagg
    20821 gcctggcgcg actcctggcg gaggtcttcc agggccacgg cggtctgttc ccggagggtg
    20881 cgaaaggcgt cctccagggc gacgatgcgg gacggggcat cgaagggggc ccccgctccg
    20941 gggcggggcc gttctccctc cttccccggg gccaggggag cccttctcag ggcctgctcc
    21001 cggcggagga gggataggac ctcttccacg gtgggtgggg gggagtcggg ggagcgttct
    21061 cctcccccgg aggcgccctc cgccagggag gcgatggggc gtcccggctg cgattcccgg
    21121 agcacgaagg ccaagaggtg ggccatccgc tcccccgtgg cgtcgtttaa cagggtgggg
    21181 tcctggcggg ccagctcttt tatgcgattg agggcgtcct ggggttccat ggcatgcctc
    21241 ctggggcgaa atggaaaaga ggccggggcg aggcgggttt ttcccctctc attcccgggg
    21301 tttttctccg aagagccaat cgatggcccc cttggggggg ggcggagcct gagagacccg
    21361 aagtttttcc acatggtccg ccatggcccc cagggcggac cggagctccg aggccagctc
    21421 ctcccggcgg tgtcgctcct cggcttgtcg gtccagcccc tcctgccgga gggagtcgag
    21481 gcccctctcg aaggtcttct gctggagcgc cagctcttgc cccatcctct tccagctctc
    21541 ccgaagggcg tcgaagtcct tccccagggc cttgcgatgc agagctacct cccgttccag
    21601 ggcttccccc ttgtcccgga aagattccac cgtttcggcc agttgagccg tggccttggc
    21661 cagggcgtct cgctcctctg cctcccgccg ttcccggtcc tggaggtcct gggagaggcc
    21721 ctgttgatag cgccccagtc ggtccatctc ctccgtcagc acttcccgct tttggacctc
    21781 gccctgcagg gcggacttca gggcgtccac cgccgccagg cggcgttcca cggcggcgat
    21841 ctccccggca aagcgcccct cttctcctgt ccgatgcctt tccaggtgga cgcagcgctc
    21901 tttcagttgg gccaggtcct ttttcgcctc cgaggaaagg gccatgagat ccgttttgat
    21961 catctttagc atctgaccct ggaactgctg ggacttggcg atttccacgt tggagctcat
    22021 cttctcctcc cggatgcggg cgatggcttc cgcctgccgg gccgtttccc tggaggtctc
    22081 ctccaaaaat gccttccagc gcgtctccat ctggccgtga cgttcctccc ggagctgatc
    22141 cccggcatcg atgcgacggc gcacgtcctg gagggcctct tccagggagc gatgcagctc
    22201 ctcttcccgg cgccgtccct cttcggccat cgctccgtct cgatcgctct ctcgggcgat
    22261 gagttgggcc tgaaagcggg ccatctcttt tcgtaggtct tccacggcgc cctgcagggc
    22321 atcgtcctgg aggcgccacg ccgcttcgcg ggtctgagac ctttcctgga gggcccccag
    22381 gtcttgggcg aggtgttgga tcttttggga cagggcggcg tggttccggg agatctcccg
    22441 gtcccagagc tctcccgcct cgcgctgtcg ccggtccatt tcttccatca gacggccgtt
    22501 ttctccggcc accagggtta gggcggcctg gagctcctct tgctggtggg atcgcagcgc
    22561 gtccatctct tcccggaggg attgccggtg gccccgcagg gcgtcctcgg cctccttccg
    22621 gagccgttcc aaggccaggg cgtttttttc ctcgaaacgc cgccgttcct ctttgccagc
    22681 ggctcggtcc tcctgtcgca gggtctccac ccgctgggcg atatcccctt gcccttggtc
    22741 caggcgctgg cggagctccg ccgtctcctg ctccaggcgg cgatttagct ctgcggagct
    22801 ctgctccagg cggtggctca tttccgccgt cccttggtcc atacgacgtc cgagctccgc
    22861 cgtctcccga tccaagcggc ggttcagctc ttccgctgct ccccgggctt cctgccaccg
    22921 ctcctccagg gtccccaggg tttggtcgat ctgggcctcc cgggcctcct ggcgggcgcg
    22981 gtcctctccc cgggcggcaa ggccctcctg tcgcagggtc tccatccgct cttccaggag
    23041 gcgacatcga tcggcgcttt cccgaagggc gagggcatag cgttccttgt cctcgatcca
    23101 ttcctgaagg gctgcggcga acctatggcg cagggcgtcg ttctcttcgg tccactcccg
    23161 gagggccgag gcgaatttgt ttcgcaacac ggcgttttct tcccggagat tcgcggcggt
    23221 gatgtggacg gagcgggcga agtcctcgca ccgggaggaa aagaactcct ctccccggtg
    23281 gcgctcttcg gtggcccggg ccaccaggga atccagagcc ttgtggtagg catcgttcat
    23341 gatttcgagg cgctccgtga actgatgccg gtggctggcg ccgtcggagg aaaggcgttc
    23401 caccgccagg cgcaaccttc ggagctcatc gtcgtaggag tgttggacgg cggcaaagag
    23461 cgcctgggcc atctgggcgc tctcctcctc cagggaaaag tacttttccg cctccgaagg
    23521 gccctccgag ggggcatcgg tgaaggggtc ttcctccggg tcctccgatg aaggggaagt
    23581 ttcgggggct tcctcgacga tctccgctgg cggcgttccc tccgcaggcg cggcgggctg
    23641 cggggccctt ttcccttccg ccggggtttc ttcgggagag gggctagcct cctccggggg
    23701 ggcgtcgttc cctccggagg cccgggggag ggggtccggc tcctccgggg cggagaccat
    23761 cgccggagtc ccggaaactc cctccgatgg agagggggta agctcccgga tggtgtcgat
    23821 aaagcgttcc tcttctcgtt cgatgttctc cagggccaga cgctgctgct ccaggatctg
    23881 gtagcgcttc agaatggccc ggaagcggcg ggggctcacc cgaagctcct gggccccttc
    23941 ctccggggag agccagtcct tgcggaggct ctccagggcg aaggtgagga cactggccac
    24001 ctgctcccgg gttccttcgt ccatccggga gaggtcgagg tgggccagag atctcaccgt
    24061 tttcagcacg tcctggggct ccatggcatg cctcctcggg ggggagtgtc gtttctcgaa
    24121 aggggacgcc gccgggggtt atccgccggg gctccccgaa gtgccctcat tatattcccc
    24181 ggagtttccc cggggcgatc gttccaaagg ggggccgcgt tagggccgcg ctcagcgctt
    24241 cttgggaggc atggactgga tgaaagacga tagcaccagt ccggccgtac cgatgcccgc
    24301 caccagcacc accccgaggg atcccacgat gcccccggcg ccgaagccgc tgtacagaag
    24361 accctgaccc gctcgggagg ccgccagaaa gcctcctgcc atgcccaaga gaatgctgag
    24421 cgtcatgaaa aaacctccga tgagggacat ggcgcccccc acgggaattt cgtgctcgtc
    24481 catgatgtcg gtccagagca ggtcccattt cttgatgcgg gggtagagca ccgtgaggat
    24541 cggcaggccc aggaccacgg cggggatggt gttattcaac acgatgatgc ttcccagggc
    24601 gccgaagggc accataccca gaagttccag cccccaggcg atgggcaggg cgcatcccgc
    24661 cgacccgaga aagctcacca ccacataggc cacgaccttg cgcctggagg tgggatgggg
    24721 ctcccggtcc tgggaaggga cgagccccag gttgatccag agtttgtagg gcacgtaggc
    24781 gaagaagaag ttccccacga agccaaagag gctgccgatg cccagggagc cgaagaagtc
    24841 cccgatgaga ttgccgatgg cggaccccca ggccccggcg gggccgaaga ggagccccat
    24901 gaccaccggc agggcgctgg cggggcgaaa ctccgtgacc ccgggaatga gcacgaaccc
    24961 cttgaaggga atcaggagcg ccgcgtaaag accggcacag agggccacca gcaccaccat
    25021 cttggtcttt ccccacatgg aaaaaacttc atacatgtct tgtcacccct ttgagaactt
    25081 cgcgagaagg ccccgcgcgg ggcttttcgc ggcctggggt tctccgatgc cggaggaacc
    25141 ccgctccaga cgccgactca gggcaagatc gccccccagc tttccagggc ctggggaaag
    25201 aacttcgcga caaggcccac cgcggcgagg gccaacgcga aggccaccgt gaggtaatcg
    25261 ccccccttca tgcgaagttc cagaaagttg gtgcgctggg gactgtagcc gaacccccgg
    25321 gattcgaggg ccatggaaaa gacgttggtg ctccggatca cggagatcag ggtgggaatc
    25381 accaggggca cgtgtttccg gatcttttcc agaaacgatc cggcgtcgag gtcgagcccc
    25441 cgggattttt gggcctgggc gatggtggcg ctggtggcca cgatgagggg taccagccgg
    25501 atcgccgtgg agaaggcgaa ggcccctcgg tagggcagcc ccagcttcac caacccctgg
    25561 gagatctcct cgatcttggt ggtgctcaaa aagaccatgc ccgcgatgat catgagatcg
    25621 gtcttcgcgg ccaccatgag gccgtagagc actccctccc aggagaacgc gagccccgca
    25681 cttttccccg gccccacgat ggaccatatg acgagggaaa agagggcgat catggcgagc
    25741 accagccgga tgcgcctcag gttggaaagg acggcccccc aggccccgtg caggagcacc
    25801 agggcgagga ggactcccag gggaggaagg gtcgaaaagg ctagggctag ggcgaagctg
    25861 accaatagga gcaggatctt cgtccgggga tcgaggcggt gaaccgccgt gtcccggtcc
    25921 atgtacagga aaagatccat taccggccct ccttcgtgca gtacgccagc tcctcggcgg
    25981 aaagcaccgg ggccccgagg agatttccca aagacacgat ggagggggtc ttgagtcccc
    26041 aggcggcgag ctccttttcc cgggaaaaga cctcccgggc ggggccgtcg tgcccgacgc
    26101 ggccgtcttt catggttacc acccggtggg cgtactccgc cgccacccac atggtgtggg
    26161 tgatgatgac gacggtgtgc cccgcctcgt tgagtttttt tatgagctcc atcatctttc
    26221 gctgccccga gtagtcgagc cccgtggtgg gctcgtccag cacgatgatc ttcggccggg
    26281 cggagagcac cgaggccacc gccacccgct ggcgctctcc cttgctcagg gaaaagggat
    26341 cctggggacc gtaatccggg atacccacgg cctccagggc ctctcgcacc cggagcttga
    26401 cctcctccgg gctcaccccc cgaatccggg ggctgaaggc cacctcatcg tggaccgtgg
    26461 gggaaaagat ctggtggtcc gggttttgga acacgtagcc gatgtcttgg ccgatctcga
    26521 agaccgagtg ggtccgggtg tctttgccgc agaccgtcac ccgcccctcc gtgggggtca
    26581 aaagggcgtt aaagtgcttg gctagcgtgg tcttcccgcc accgttatgc cccaaaaggg
    26641 ccacgaattc cccctggcgg atctccagag agaccccgtc cagggcgcgg gtcccctggg
    26701 gataagtatg ggataggttt tccacccgca ccaccacggc gccgtaggat tcttgccgct
    26761 ttcggtcgcg ctcccggagc tcctggacgc gcccctcgtc cagccggagc tcttgacggt
    26821 ggaagaaggc ggccccctct ccgggtcgca ggggaagggg gtcttcgctc cccagggcga
    26881 gccccgcgaa gtaggaagga atggccaggg gcatgatccc caggccctcg atgagctcca
    26941 catctcggag gagatcggcg ggccttccct gggctacgat gcggccgtct ttcatgagca
    27001 ccaggcggtc ggcgtgcagg gcctcttcgg tctcgtgctc gatgatgacc agggtaagtc
    27061 cgtcactgtc atggagctgt cgggcgatgg taaagacctc ttccttgccc cggggatcca
    27121 gatcggtggt gggctcgtcc atgcagatca cccgagggtg ggcggcgagg accgccccga
    27181 tggccaggcg ctgcttctgt ccccccgata gggtggcggg gtgcctcccg gcgaagcccg
    27241 aaagccccac cgtccggagc gttccctcga cgcggcgggc gatctcctcc ggagggacgg
    27301 cgaagttctc ggggccgaag gccacctcga gatccacgtt ggtggaaaag atctgggcct
    27361 cgaagtcctg gaacaccagg ccgatctccc gggccatgtg gctcacgggg gtgtgccggg
    27421 ggtcttgccc ccccactcgg atctctccgg agaccgatcc ccggacgaag tgggggatga
    27481 ggccattcag ggcatgggcc atggtggatt tccccgcccc tccggggccc atgagggcca
    27541 cgaactctcc ggggaggacc tcgaagtcca catcccgaag cgcggggagc ccctcggccc
    27601 ctcgatagga aaaagaaagc ccctggaccg cgatggcccg gggaggaccg ctatcgtcca
    27661 cggcggtaac ccccttcggt gacttgtggg agcgcctctg aaatccccac ggaaacctga
    27721 cattacgacg ccccgatgag cctgtcaagg gcggagcgtc ccgccgtaga cctcctcggg
    27781 gcgcatggcc gggatcgcgc atcttcccgc cgacccgatc ccaccctttg cccggagagg
    27841 cgacagggtc aaacctttta ccccacggtc ccttcgtttg cggtaaaata ccaccataga
    27901 agatcgtccc ggggtcgggc gagatgaaga gagtgttcct cgggccgccg aagatgagcg
    27961 ccctgaagcg cctcctccgg actccctcgg agaaggggct gttttatctg aaacaatgcg
    28021 gagggagaag ccatggacca gatcgccaaa gaggttccca tgatccaaaa ggcccgcacg
    28081 atggaagaac tcttcatgca gatggagcac gagcggcggc ttttacgagc tccgagaaaa
    28141 gggattgcac ggcatcgcca ccctgcagaa acacgcccgc caggaagggc acaaagaggg
    28201 catcgagaaa ggccgtagag agggcctccg tgcctcggcg cggaacatgc tccgaaaggg
    28261 catgccgatg gcgctggtgg ccgaagtgac gggactttcc ccggacgagg tggcggcgcc
    28321 gagggattgc gtcaacagcc agcaataatt tcacccttct ggcgccttcg tggtcaaaaa
    28381 atatcatgac gatgtagatg ctctggcccg cgagagagag gtgggaatat gaccggattc
    28441 aaagaaaagg ttcagttgca ccgaacgctg ctacggagag gagtctcttt gatgaagggc
    28501 ggcggattgc tgctgttgct tttcctcgga tcgacggtga tgctcggcgg atgcggcgga
    28561 ggcgacgcgg cgcatcgcga gacgttgttc gtcaacggcg ccatctatgt cgatgcggac
    28621 agcaaagtgg acaacctttt gattcgtgac ggcatcgtcg cggggcacaa cgtcgatccg
    28681 aacgagcatc ccgaggcctc cgtcgtcgac ctgaacggag cggccgcgta tcccggattc
    28741 aacgacagcc acgtgcatct tgtgagcatg gccgtggcgt aaagcatcct tgcggaaagc
    28801 tccgcccttc gcgaaggcaa ggagcgccct cggcccgaag attttttcga cgacatccgc
    28861 atccggtgca gccaggttcc gaagggaact cccgtcatgt ctcacggatt cgtgctcgac
    28921 gactacgatg cctgggggtt ggcggacctc gccgcccttg atgccgcgtc gggaccggac
    28981 cacccggtca tgatggcgga ccagttgggg cactcctaca tcgtgaattc ggcggccatg
    29041 cgcatcagcg gtctggatgc cgacacggtc gcaccgccgg gaggaaaaat cgtcagggat
    29101 ccggcgacag gagccccgac cggcatgctc cgggaaaccg ccggcgccct cgtggggaac
    29161 gtcgcgatct ttccccaggt tcccgaccag atcgtccggg aaccgcttct caagcttctg
    29221 cacctatggg cgtccatggg gtacacctcc atcgtcgagc tgatgggagg tccgatgggg
    29281 cgaaccatga aacccggact tcttcgggat cttgagggcg aaggaaaact gcctctgcgg
    29341 gtctgctacg catacacctt caccagtctg aacgatcttg aagggtataa agatgccggg
    29401 ccggataccg atctggtgca tttctccggc ctcaagctct tcgtcgacgg cgctgcggga
    29461 cagggcggcg cgtggacgag ctgggagaac cagcttgagg atccctcgca aagacacgga
    29521 ttgcacgccg ttctcaccga agaggtcgag gggcttgcgg cgtacaacat tttcagcatc
    29581 gtcgaccgct ccgaagagct ggggttggac gttcactacc atgtcggagg cgatctggcg
    29641 attcaggccg tgttggacgc gatcaacgcc gcaaagaaga aaaacggccg cttcaagggc
    29701 tcccacaccc tctatcatct tggttttctc accgaccctc agatagaaga gatgaagctc
    29761 ctcggcgggc acgtgatcgc gggaatacag ccgtcgctgc attggaagaa cgaatggaaa
    29821 aacacggagc gcttctacgg accgtatgcc cgctcggcgt atccgtatca gaagatgctc
    29881 gacgccggca tcaggcttgc cttctcgacc gattttgcgc cgaacacggt gctcatgagc
    29941 tacccgaccg aaatcatgcg tgttgtgctg accgggggag aaggacgtcg tcaaaacacg
    30001 atctcgatgc gcgacctgat cgaggggttc acctccgggg gcttcgcgac gacgcctctg
    30061 acgaacacgg ggaaactgca catcggctat aaagccgaca tcgtggtgtt cgagaaagat
    30121 ctgtaccatg tgcctccgca acaactctcg aaagactacc cccgcgtcct cggaacgtgg
    30181 gtcgggggcc gaaagatcac tgcggaccgc tgagcgccct cgccatagaa aagagtacgc
    30241 ttcagcgccc gtgatacgcc ctccgcaacg tggacaacat gcgaggggcg gagcttcttc
    30301 ttcaggtatc ctttggcggc cctgtgggtt taggattctt cacctgggtc ccctggttca
    30361 acccgatctc ttagcggagg ggttggattc ggggactctc ctttttccca aaaccttacc
    30421 ccgaagggcc atttatgtga cgcacttccc ggagagatag agggagcggg aacagcttga
    30481 cgtggagcgt ccacaggaaa aggggcgcaa tcggtcttct gccccctttc cccacgcccc
    30541 cggccgttct tccggcggcc gaaggtcccc ggaggggccc ggccgccgga tcgacgctgc
    30601 ggtggggtgg gatgttacag agggcgtacg ggctcgcaga tgtccaccac gaactttcct
    30661 tcggggtggt tctggggatc ctggcggtag atctcgtagc acatgcggtc gtcgggctgg
    30721 taccccgaag aggggaacca atctcccatg aaggtctccc aggcttcgcc gaactggtgg
    30781 ggaaggattt caaagcgggc cacagcgtag gtgccccccg gaatggtcat gaggttcacg
    30841 tcccccgaca ccgccgtgtc cggcggcacg gtgaggcatg cgctggaccg aagcttggat
    30901 tcttcggtgg tctcggggca gtcgtgatag acccccaggg tcaccgctcc ggggattccg
    30961 aagagccccc gggggcccgc ccagcgggac aggcgctcga aggccgcagg gatccccgag
    31021 taggggccga tgtggcgcac gtaggccacg gtgagttccg gaagttcctt gacctccacg
    31081 gtgtatgcca gctttgccat cttgatcttc ctcctttttc cctcgtcgga gagggtaaag
    31141 gaataatagc gcccttcggc ggagcgtgct tgctctccgt tgccctccgc ttgatcctcc
    31201 gtgcgatccg tttgatccct gttgctctgg tcccgagggg ggttcgtcgt ccggccccgc
    31261 cgccgccact gggacgcggt gacgccaaaa cgctcccgaa aggcccgggc aaagacgctg
    31321 gagctggaaa accccaccgc cagagccact tccgtcaccg actcctcggg gcggtcaaaa
    31381 agacgcgcgg cggcgtgttg caggcgtatt ctcttgagaa attcccccac cgtctccccc
    31441 acgatgccgg aaaagatgcg gtgaaagtgg aaccgggaaa atcccgccac gtcggccaga
    31501 tcggcgaggc cgatgtcctc gccgtagtgc gcgtagatgt ggtccacggc gcgttcgact
    31561 cttccgtagt actcgcgttg gagcagctct tcccgggtca tggggacctc ctttgggggc
    31621 gcgccgccgg gggcgatcgg gctttccggt cccgcgccgg aattgcccgg ggcgccaggg
    31681 ccgaggaggc cattgtacat gctccagccc cccggcggaa agccttgggt cggcccttcc
    31741 ccgggagccg gggctttatt cgtgccgcag ggcctcgatg ggatccagca gggaggcctt
    31801 ccaagcggga taaaatccga aaaacacccc caccaggccg gaaaatccga aggaaagcag
    31861 agccacgggg ggggagatct gggtgggcca gtccagccag gcggtgatgc cccgggaggc
    31921 cccggccccc aacaggatcc ccacccctcc tcccagaagg gagaggacca gggcctcggt
    31981 gagaaactgc caccggatgt ccccggggcg ggcccccacg gccatgcgga tgccgatttc
    32041 cttggtgcgt tctgtgacgg aaacgagcat gatgttcatg atgccgatcc cccccaccag
    32101 caaagacacc gaggccacgg cccccagcaa gagggacatg atgcgggtgg attccgccga
    32161 ggcctccagg atgtccgtca tgttccgcac cacgaagtcg tcgtccccgt cgtgggggat
    32221 gcggtgccgt tggcgcagca ggacggtgat ctcctcctgg gcggtgagga cggactccgc
    32281 gttccgggcc ttgacggtga gccgccgtac cgccccgggg atggagctgc ggaagaggtg
    32341 ccgttgggcc gtggtgaggg gcatgaggac cacgtcgtcc tgatcctgtc cccagggggt
    32401 ctgccccttg ggagccagga ctcccaccac ctcgaagggg gtcttttgga tccgcacggt
    32461 ctttcccacg acctcgccgt ggccgaagag gttctcggcc acggtggtcc ccaggaggca
    32521 gaccttggcg ccgtttttca cgtctccgtc cccgaaaaat ctcccctcgg cgagacgcca
    32581 ctcccggata tcgaagatgg ggggcgtggt gccggtgatc ggggcggacc agttcatgtt
    32641 ctcgaagacc acctggcccc ggccgaaggt ctcgatggcc acggcggcca ccgaggggca
    32701 ctccagagcc atggcctccc cgtcgtcccg ggtgagggcg ctgccgctgc cggcggaccc
    32761 ccgtaccccc gaggtgttgg gggctccggg aaagacgatg aggatgtctc gccccacact
    32821 ggccacggag ctctcgatgc gggcccccgc cccctggccc agggccacca tggtgatcac
    32881 cgcccccacg ccgatgacca cccccagcat ggtgagcaga gatcgggcct tattccccat
    32941 gagggaccgc accgccgtgc gcaggacctc cagcatgttc aggcctcctt tggggggcgc
    33001 cccggggtgg aaaggtcctc caccagccgc ccgtcggcga aacgcaacac tcgccgggcc
    33061 caagcggcga tatccgcctc gtgggtcacc agcaccacag tcttgccctc ccggtggagc
    33121 gccgcaaaga gctccatgat ctcgtcgctg gtatgggaat ccaggtttcc cgtgggttcg
    33181 tccgccaaga tcaggggagc gtccgccacc agggcccggg cgatggccac ccgttgctgt
    33241 tgtcccccgg acatctggtt gggacggtgt cccttgcggt gctccagccc cactcgatcc
    33301 agcatggcgg aggcccgggt ccctcggagg cgagaggata ccccggcgta gagcagggga
    33361 agctccacgt tttccagggc cgtggcccga gggaggaggt gaaagccctg gaagacaaag
    33421 ccgatcttct cgttgcgcac ccgggccagc tcgtcccggg agaggtgccc cacatcccgg
    33481 ccgtccaggc ggtaggtccc gtcggtgggg gagtccaggc accccaagac gttcatcatg
    33541 gtggatttgc ccgaccccga gtgccccatg atggccacaa attccccccg gagcacggtg
    33601 aagctcacgt ggtccagggc gcgaagctct ccgtcgccgg tgcggtagat tttggtgacg
    33661 ccccgaactt ccaacagcgg gggaatttcc ggggcgcctc cttgggtccc cggggaggtc
    33721 atgggccccc gccggaggcg aggaggcgct gccaccggga ggtgccgggc accttcgtcg
    33781 ccgaggtgat gatgggttgc ccctctcgca ggtcccccga caagatctcc acgaaggatc
    33841 cgtcggagat ccccggaacc acggccacgg gccagggggc gtcctccgag aggacccaga
    33901 tccggggccc cgatcgcccg gcgccatggc gtccgccgtt ggggcggcgc gggaaggtgg
    33961 gccccatccc gggcatggcc cccgaggcgg aggaggaggg ggcttcgggg ggggcaaagc
    34021 gcagggccgc cgcaggcacc cgaagcacct gggggcgacg ttccgtgatg atggtgaccg
    34081 tggcggtcat gccgggcttt agcaggagct cctcgttatc cacccggatc acggtggtgt
    34141 aggtcaccac gttttccgtc actgcggggg ccagccgtac ctgggtgacc gtgccccgaa
    34201 aaagttttcc tgggtgggcg tcgacccgaa actccacgtc ctgcccctcg gtgacgcgcc
    34261 cgatgtccgc ctcgtccacg tgggcttcga tctgcatccg ggtgaggtcc tcggcgatgg
    34321 aaaagagggt gggggtctgg aagctcgccg ccacggtctg ccccacgtcc accttgcgat
    34381 cgatgaccgt gccgtccgtg ggggagatga tgcgggcgta tcccaggttc tcccgggctt
    34441 gcttcaggga ggcccgggcc tgggccacct tagcctgggc ctcctggcgt ccggcccggg
    34501 cggtgagcag cgtcgtttcc gccgcatcca cctcgctctg ggcgatgagg cgacgggaaa
    34561 agagctcctt ctggcggcgg aaggtgcgct ccgcgtcggc cacggtggcg ctggcccggg
    34621 ccacgcccgc ctgggccagg gagagattgg cctcctgctg ttccacctgg gattgaagga
    34681 tggaaggatc cagcagagcg atgacctgcc ctcggtggac ccggtcgttg aagtcgacgg
    34741 aaatggccat gatggtgccg gacacctggg tgcccaccgc caccacggag accgcttcga
    34801 tccgccccgt ggccgtcacc gtcgaaacca tgtcctgccg ctccaggggc acggtggcaa
    34861 attggacccc ctcctccccc ggggccaggg cataccaacc cccgcctccc cccagggcga
    34921 cgacgaggga aaatatcaga agttttttca tggaatgggc tccttccctc ccgtggcctg
    34981 ctccagatcg gcccgggcgg cggcggcgcc gtagcgtatc ctgtaagacg ccaggcgggc
    35041 gtcgttcagg gccagcacgg catcggtgac ttccagggcg ctgcccaccc ccgcctcgta
    35101 gcgcccggtg gccagggcca ggttctcctc ggcctggcgc accacgagct cctggttctc
    35161 ccgctgggcc tcggcctctt ccagggagcg cagggcccgg cggatctcca gggcgatgcg
    35221 ctggcgcagc acctccgaag ccgccgccag ggagtcctcc cgggcccggg attcctccgt
    35281 ggcgccccgg gtggcccccc catccgccac gggccaggag acggtgaggg agacccggcc
    35341 attttcctcc aggggaaagc gggccccctc gtacccccag gagccctgga gccgggcagt
    35401 gggggcgtat ccccgggcgg cggcctcggt cttgaggcgc cccgccctct cctggagccg
    35461 ggcggcccgg agatctcccc gacgctccag ggcctgggcc catagctcgg cttccggggg
    35521 tacgggggat agatcggggg agaggtcgac ggttttgaag gggggcatgg aagagatccc
    35581 catggcgttg accagagtgg cctccgcgag atcccgggcg gagcgggcct tcaccagggc
    35641 ggtgtgggcg ttgccccggt ccacctcggc cttggtgacg tcgatgcggg acttcgtccc
    35701 cgcctcgtaa aagccccggg cggtggcgag gtgatcttcg aaggccttct ctgtggcctg
    35761 ggcctggcgc tccgtggcct cggccaggag aaggtcgatg taggcccgct tcaccgcagc
    35821 gcggagggtc tgatttaaat ttatcctctt ccaccgggct ccctccacgg cgagctcctg
    35881 ggcctcctct tcccgatggt cccttcccgc gtcgtagatc ttgaccccag cggcgatgcc
    35941 caggccgtgg ctggcctccc ctcgggcggg gtggctctcc gtggcggtgg cgtccaccaa
    36001 cacccctttc cgggcggcga tctgccggag ccgggcccgt tccatgcgct cctcggcctc
    36061 tgccccccgg agctccgggt gatgggcctc cgccatcgcc aaacaggcct ccagggagag
    36121 gaccggggga tcgctccgat cggcctccgc ccccgaggcg aaaagtagga gagccagaag
    36181 gacccctggc ccgctccgga gccggaacgc ccccccggag atttttctag ggaaatgggt
    36241 cacggaaaca cgctccttcc ccgcccgcag cggctccgag gaccccaggg ccccgatgct
    36301 ttcgggcgtc ttttttagag taccacgccc cggaggcccc ctggagggta aaaatacggt
    36361 atgattttac cggttaggcc ccgaaaaagg ggagctttcc tccccggggc aggaaggggc
    36421 aaggcgaggg aaggaatgtg ttagaatgga cctaggcgct ccgggcgcat cacgaagggg
    36481 cgcagcaaag aatagctgtc gatctatccc gataaagggc gcgggattcc ggggcgcccg
    36541 ccgggacggg tcgggaagga ggctccatgg cgcggaaaaa agtggtgggg ttggtcatcg
    36601 gtgctctgct cgcggtcgtg gtgggcgggg tgtttttcgt cgacggagcc acggatctgg
    36661 tgaaagacca ggcccagtcc gccgtgcggg attcgttggg gggcgaactt cttctggggg
    36721 acgtgacggg aaaccccctg cgggggtaca cggtgacccg ggtggccctg gaaaaagacg
    36781 gcgtgaccct ctttgaggcg gaccggatct ctctggcggt gtctcttttg gccttcttgc
    36841 gcgggggggc catcatctcc gacgtggaga tcgcgggggc ccggggggat gccgaccgcc
    36901 tggggaggtt ttttgccacg atcaagggcg aagggggcgg ggggagctcg ctggtgggtc
    36961 gggtgacctt ttcggacagc gtcctcacca cccccgccgg gacgcttttg gcctcccggg
    37021 cgaggatctc cctgggggga tcgacggtgg ccctggagct cgacgggcga tggcagggtc
    37081 ccggcaagac ccccctatcc ctttcgggga aggggacgct cgcgttccaa ggggacgccc
    37141 tggccgtggc agagctggat cttcgcgtgg gtcaggggaa ggttctcctc caggggagcg
    37201 tgctccccga gctggggctg tggggcaccg tggagggcct cgagggatcg gaggtgggag
    37261 ccctttggcc cgagaccgag ggggcccttc ggggaaggct ttccctggcg atccagagcg
    37321 ggggcacctg gaagaacccc cggatctcgg gggacgccac cctggaaggg ggaaagttct
    37381 ccggcgtgac ggtggaccgg gcctcggggc gtttttcctt cacccccgcc ctgctcaaag
    37441 tcgccgggat cgaggcctcg gtcttcggcg cctcactgcg gggcaccctg gcgttgggcc
    37501 tgcgcaacct gccccccacc gtggcgcttc agatgagggg cgccaaaggg gatcttgccc
    37561 aggtggccca ggccttcccc gccctggggg accttcaggg gatggtggaa aagatctccc
    37621 tggatctgaa gggatcgggc tccgccctgg agggtcggat gaccctggtg gccccctccc
    37681 tgagcggccg ggggcataag ctggagcagg tggcggcgga cgtgaagctc cgcgatggaa
    37741 agaccttcca cgtcgccgcc cgggcccagg ggttcggctc ccccctgacc ctctccggca
    37801 cggcgtcctg ggggaagaaa atagacatga atttggagtt taaggcgacc aggctggatc
    37861 tggcccgtct agaggccctt ttccccgagc tccgggagct caaaccccgg ggagaggcga
    37921 gcgtggcggt gaccctcaag ggggatcccg gagcgcccca gatttcggga atcgtctccg
    37981 ccccccagat cattctggcg ggggagacgc tgagcaagcc ggtggtcaaa tttgccctcc
    38041 gggggaacac cctggcggtg aacgccgcat ccctcacctg gaaagggggc gttttttccg
    38101 cctcggggag cgtggggggg ctcgggggca aaggggtgac cctagaccta aagggcaaag
    38161 gagaagccct ggcgctggaa aaccttgccg gggccttccc ggcgctgaag ggatcggggg
    38221 tcaaggggac ggggacgctg agctggaagg ccagtggctc gccggaaaag atcttggtgg
    38281 aggccgccct ctcctggaag agcctgcggg ccctggatca gctgggggtg agcgacctgg
    38341 aaatccgggg atcggggcgg gtgacgggag gaaagcccgc cctcgaaggc cccctgaccc
    38401 tccgggcggc gcgcctggcc tgggccgggg cgggggtgga tcgcccgacc ctcgccctcc
    38461 gcgccgaggg aaaccgcctc gtggtggagc ggggggaagg gaggatcgcg gggggctccc
    38521 tggcgctttc ggggacggcg aagctgccgg aaagggccgg agaggagggc gcgctcgatt
    38581 tcaagggatc gatccgggat ctggggttgg gggccctggg caaggatctg ggccttcccg
    38641 tcgccttggg gggacagggc accgtgaatt tttccctggg gggaggcttg gcggctccgc
    38701 gcttcgccct ggacgccacc ttccccacgg tgacggtgga gggggccgcc ctggcggacg
    38761 cctcggtgtc cctggaggga accccgggag aaatccgcct tacctccgcc cgaggcgcgg
    38821 tggaggggac ccccttctcc ttttcgggga tgatgcgccc cggaccccag gggtacgccc
    38881 tggaccttaa gggctcgggg gaggggttgg atctggcctc cctgctccgg cgggtccccg
    38941 cccttcggga caaggggatt cagggcaaga tgaacctccg ttttttcctg aagggaaccc
    39001 cgggggccct ggaggggtca ggggagctca cttcggagcg ggtgggcttc ctggggctca
    39061 gcgccacggg ggtgaaagcg cccttttccg tgcagaaaaa cgtcctggcg gcccccgcga
    39121 tcaaggccct cttccacggg ggagacgccg ctctggggct ccgcatgaac ctgggcaccg
    39181 gaggatggga gctccaggcc gctttggccg aggtggacct gggggccacc ctgcgggccc
    39241 tctcccaagc ttcggggacc gtagggggta ccgccaccgt ggatttcaag ggcaagggca
    39301 acgccttcaa gggagagttc tcggggaagg ggttgttcca atcccgggaa ggagccgtga
    39361 cgggttatcc ctgggtgaac ctcgtcgcca ccctgcacgg ggcttccggg gtacgctacc
    39421 agctcctcaa ggctcccttc tcggtacagg gggcccagct gaccttgcag gagggcacca
    39481 aggcggtgcc ctgggagggg gatccccttt accgaagcct cgaggcccag ggcaccgtgg
    39541 ggcccgaggg taagttgaac ctggcggtga agggcgtcgt gaatgcccag gtgctcaacg
    39601 ccctcacggg aggagtgcag ggggggcttt tggagggcat cggaggggga gcctcggtga
    39661 aagacatcct gaaaggagcc ctgaagggct ttgccgacgg aggggcgaag caggattttc
    39721 gcgccgtgag cctcaaggtg gggggaaggg cggcgagccc caagatatct gacctgcagg
    39781 tggagtcgaa ggctcccggg caaggggacg gcgcgtctcc ctcccccgag aagcccgctc
    39841 cggcgccgga gaagaccatc tcgttgccgg ggggcatcga ggtgcccctt cccgacggca
    39901 ttttgcccga gggccccaag ccatctcctg aagaagggca agcccccgtg gaagcggagc
    39961 ccacggcggc gccgaatctg gaggatgcca tcaaggacgc cgtgctggac acccttttta
    40021 aatagaactg agatcccgtt gttgggggat cacccggcga ggcggaggag atgcgccctc
    40081 cggggagttt aacggaagaa gtggcggggc ctcccatttt ttttgacgca ggggcttggg
    40141 agtggtattt ttagtccagc ggggatcctg tgatctcgcg accacgaaaa ggagcgatct
    40201 cttgtgccgt ttttgtcccg atcctacctc cgccccctgt tgctcgatat agtggcatct
    40261 cccagcgtct ccccctccgg cgaccgagaa aatgccgtgg cgcggcgcat cgtggaacac
    40321 ctggccgatt tgccctactt ccaagaacac gccgaggacc tcatgctcct tccctgggaa
    40381 ggagacccct tggggcgcca cctggtcttt gccctggtgc gagcccccga gggttcgggg
    40441 gacacggtga tcctcacggg gcacatggat gtggtggatg tcaaggtctg tggccccctg
    40501 gctcccctgg ccttcgacgc cgaagcctac accgcccgca tcggagaaat gcccatggac
    40561 gacgacgcgg cccgggatct ggcctcgggg gaatggctct tcggccgagg ggtggcggac
    40621 atgaagtgcg gcgtcaccgg ctgcatcggc gtgttggccg aggcctccac ccggcgggag
    40681 accctgcgca cccacgtggc ggtgctcttc gtccccgacg aggagaacag ctccgggggc
    40741 atgctcgccg ccctgcccca cctggagcgg ctccagcggg agcgggggct gcgctatctg
    40801 gcttgcatcg atacggaacc ctccatcggc atgggggacg aggccttggc ccgggtgtac
    40861 accggatcgg cgggaaagat caatcccttt ttcctcgtgg tgggatgcga ggcccatctc
    40921 ggcgagtact acgaggggct caactccgcc gccgtgctgg gggagctcct gcgcctggcg
    40981 gagggaaacc cggacctggc cgacgagggg ggcggcgagg cctttacccc cctggcctgc
    41041 atgaagcacc gggacctgag gggagagtac tccgcctcca tcccggagcg ctccgtggcg
    41101 tacttcagct acttcaccgc gagcaaaaat ccctgccagg ttctggatct cctccggtcc
    41161 ctggccctgg aggcccagcg aaacgccctg gatcgccagg aagaggcctc ggcccgtttt
    41221 gccgcccggg gcaagccccg ggtgcacccc agaccccggg aggcccgggt gatgaccttc
    41281 gaggagctcc tctccaaggc ccgggatcgc gtgggtcccg aagccgacac cttggtggaa
    41341 agggccgccc gggaagctcc gggggatgag cgggaccgtg ccctggcggc ggcggatgct
    41401 tgcgtgaccc tggcggggga aaaggggccc ctggtggtgg tgggcttcct catgccctgg
    41461 tacccccatc ggggaaaccg gggggaaacg gtgggggatc gggccatgct ccgcttggct
    41521 tcccgcgtgg cggcggaggc ccgggatcgc ttcggcgtgg ccctggaaat tcgccccttc
    41581 tacgaaggta tctcggacct gagctactgc ggatactccg aggatcccga gggcatggag
    41641 gcctatatcc gcaacgtccc cgcctacggc gtggattacc gtctgccggt gcaggagttg
    41701 cagacgttgc acatcccggt gctgaacctg gggcccatcg gcaaggatgc ccacaaacac
    41761 accgaacgca tccacgaggg gtatgccttc gacgtcttcc cccggatgct ccgccgggcc
    41821 gtggaactgg tccccgccat gtatggggga gagtaaagac aaaggggttt cgaaggaccc
    41881 ggctcctccg aaaccccgcg ttttttaata acaaattgtc gccgccctcg cgcctttggg
    41941 cggagctttg aggggggggc tcaggccggg ggaggattcc ctcgacgctt ccggcgcagg
    42001 tggcgcacca ggaagagctc ccccacgaag aggatcccgg cgatgaggta gagcccccag
    42061 gggccggaat ctccggcggg ggtgttgctc agggcctcca ggagccgctc tccctcccgg
    42121 cgcaagtccc gggggacgcc ccgttccacc aggtccccgg taaagtgggg catctcccgc
    42181 tccaggatcc ccagagcctc catccggtcg gcggaagaag gagattgggc gagggcccac
    42241 gcccagtagt agctccccac cccccggacc ggggcggggc gccccacgga gcggaattcc
    42301 cgctgctctt cgtctcccga ggcgtcgctt cttcggggaa aggcttcgtc gagccactcc
    42361 acctcgtcgc tttcgtagcc atcgccgtag gaggccaccg ccgcccccac gtccagggag
    42421 agccccttct ccgccgccag acgagccgcg taggcgaagg ccaagggctt gaggggggcc
    42481 cccgtctgga agttcgtggc gtccccatag gaggacaaga tccccaaaaa gggcccctgg
    42541 gggtactcca ccgtcgaagt ccggctggcg gcgatgcccg ccaccaggag gtaggtgggt
    42601 ttttccggcg atccgcactg ggccttcagg aatcccgcgt gggctccggc gaaatccccc
    42661 tgatcatagg aagcccacga cacgcgtggg tccacggagg accctcaccc cgcctcggcc
    42721 cctcctccca ggccaagccc cagggcgaac cccaaaaata cccccatcca cccgcgtcgt
    42781 ttcatgctgt gccttgctcc cttcgacctt cttttcgatg gccatcggcc cctttcgaaa
    42841 ccccggatcc tcttcgccct cccggcgcgc catgccccta tttgtcagga gaacttcacc
    42901 gatggagtct cccgttcagc aggattttcc atctcgaagt actccgcctt ggagaagtgg
    42961 cacatccccg ccacgatgtt ggagggaaag gactccaccc ggatgttcat ctcccgggcg
    43021 gcgccgttgt agtagcgccg ggcgctctgg atctcgtttt ccgtattccc cagctcctgc
    43081 tggagctgga ggaagtttgt gttggccttc aggtcggggt aggcctccgc cacggcaaag
    43141 aggttcttga gcgccccgga aagccccatt tccgccacgg cggcgtcccg ggggttcccg
    43201 gcgccgatgg cggcgttgcg gaacttggcc acgtcctcga agatctgttt ttcgtggccc
    43261 gcatagcctt tcaccgtctc caccaggttg gggatgaggt cgtgtcgccg cttgagctgg
    43321 acgtcgatgc cgctccaggc ctccgccacg aggtttttgc ctcggaccag gccgttgtag
    43381 gttcccacgc cccaaagggc tgccaccaaa aggactccgc cgagaatcgc cgccgtcagc
    43441 atgggtatag cctcctatcc attttggtat ggtgcgccac gtcccaccat agcacgtttt
    43501 cgtccccggt ctcaagctcg ccgaatggcg gaaaaaacgc cccccctccc gggggaggag
    43561 ggggggagat gccccgcgtg ggggggctcc cttggcgtcc ttcgggggat gccttcgggc
    43621 ccccgcgaga agttaccgga gtcgcaccat gtggaagcgg cgcttgccca gacggaagaa
    43681 gaggtgaccc ccgaagagga tgtcttcggg gacgacgctt tggtcgtccc tttccacctt
    43741 gcggccgttc agggagaccc ctcccccctg gatcaggcgc ttggcctctc ccttgctggc
    43801 gcaggccccc gacgccacga ggagatccac cacggcgcgc tcttcccggg gggcctctcc
    43861 tccgggaact tcggcctcca gggtggcaag catctccggc gtgagatcct ccaggtcgaa
    43921 cttgccgcca aagagaattt ccgtggcccg taccgccccc gcgagggctt tttctccgtg
    43981 caccgtccgg gtgatctcct ccgccagggt tttctgggcc tcccggcgtt ccggagcggc
    44041 ggcgtgccgg gcggcgatct cccggatctc ttccagcgga aggaaggtga agaggcggag
    44101 caggcgctcc acgtcccggt cctccacgtt cacccagaac tgataaaact tgtagggcga
    44161 ggtgcgtttc ggatcgagcc acaccgctcc cccctccgtt ttgccgaact tctgccccga
    44221 cgaggtgagc agcaggggat aggtgatgcc gaaggcttcg ccgccggttt ttttccggat
    44281 gagatcgatg cccgacacaa tatttccctg ctggtcgttg ccccccatct ggagcttgca
    44341 cccctcgccc tcgaagagat ggagaaaatc gtaggcctgg aggagggtgt aggtgaactc
    44401 cgtaaacgaa atggacttct ccggatcgtc caggcggctc cggacgtatt ccttgttcac
    44461 cagatagttc atggggaagt acttcccgat gtcccggaga aagtccagca gggggagctt
    44521 gcccagccag tcgtagttgt tgatgattcg ggcgctgttt tcgccgcagt cgaagttcag
    44581 aaaatgggcg agctggggat ggaccccctg gaggttcttc tccatctgtt ccagggtgat
    44641 gagattgcgc tccttgctct tccccgaggg atcgccgatg agccccgtgc ccgcccccgc
    44701 gatggcgatg gggcggtgcc ccaggcgctg aagccacgcc aaccccatga tggggatcag
    44761 gtgtcccacg tggaggctgt ccgctgtggg atcaaaaccg atgtacgccg tgacggtctc
    44821 gcgctcgaag acctgagcca gctcttcggg ggcgctgcac cactccagaa gaccccgttc
    44881 ctcgaaaaac ttcaccacat tttgccccat ggtgccactc ctctctcgtt ctttctgaaa
    44941 agaaatcccg ctgcgcccgg ccttttttcc acacaaggcg ccgtcctttc cagggggctc
    45001 ctgcgaggga catcaggagc cgacggatcc gggcgcgccc tcgggtttcc tcggcggggt
    45061 ggaaatcttt caggcctgga tatcctcacg ccaaggacgc caagtgtttt ttccaaaaaa
    45121 ggttcggcat gttctcccca gaccgggctc cggaggattc ccccaacctt ggcgccggtc
    45181 accgcttcag ggccaggggc ttcggagcgg gcttggcgtc ctgggtgtga acgcgaaaat
    45241 gagccagttc gtcgcagatg tccttcgatc cggcggaaag ctcccgggcg gaccgggcca
    45301 cctcttccga cgccttggtg gtttcgtccg tggcggtgcg gatcacttcc aacgtttcca
    45361 caatgtggct cgtgccctgg gtggcgctgt ccaccgcaga ggccatctct ccgctcgccg
    45421 ccgcctgctc ttcggaggct gcggcgatgt tctgcatggc gtcgttgatg cgggcgatct
    45481 cccgcagcac ctcggcgatc tctcgccccg cttcttccgc ccccttcacc gtggcttcgg
    45541 cgatgcgacc cccttcgtcg gtggcgtcca gggcgttccg ggcctgctgc tgcaaccccg
    45601 tgatgatggc ttccacttcc ttggccgcca cgttggattc ttcggcgagc ttgcgcactt
    45661 cttcggccac cacggagaac ccccgccccg cctcgccggc ccgggcggct tcgatggcgg
    45721 cgttcagggc caggaggttg gtctggtccg cgatgttccg gatggtggcc acgaacttgg
    45781 tgatggtgcc cacggaggtg gccacctgat tgatagtttc ccgggtcacc cgggctttca
    45841 cccccacgcc tttgacatcg tccaccacct gcatcacccg agatccggcg gtttcgctcc
    45901 gtttggtggt gcggagcgcc gtttcggctc cttcggaggc ggagtgggcg gagctggtgg
    45961 ctcccgaaga gacctcttcc acccccgcgt tggtctgctc cagggcggcg gcgttggctt
    46021 cggactgggt ggccacctcg gccacggagc tccggacctc ttccatggac gccacggttt
    46081 cctgggaaag ggacgccagg gcgtccgcct ggactgcggc cgtttcggcc ttttcccgga
    46141 tggccgccat gctccggcgc aatccctggg ccatggaatc cagcgcccgg gccatttggg
    46201 ccacttcgtc ctctccccgg gcctcgaagc gcaccgtgag atcgccgtcg gcgaaccggg
    46261 cgatgtgctc ggcgaagcgc cgcaccggcc gggcgatgct cccagcgacg agcatgacca
    46321 cgacggccac cagcacgatg cccgccaggc ccacgaggga cgccaggagc gtcatgcggt
    46381 ttgccccctc caggatcacc gaaaaggggg ccatgaccac caggctccag ggggtcttgg
    46441 tatgtcccgc cgccatggga acatggacct tgtagacgtc gccattcaag gcggcggagt
    46501 agtccctcag cgccaggacc tgccccttct ggatggcgcc gatgatggtg tccgcctgac
    46561 ccctagacac gtctcgcacc gtttttccca aaatctcggc cttggggtgt ccggcgatga
    46621 ccccattgaa ggaaagcaag gtgaggctcc ccttccctcc gaagggcttt tccgaggcaa
    46681 tgatctgctg gacggtctcc aggctcatgt ccacccccac cacgcccagc gttttgcctt
    46741 ccaccacgat gggcacgcag tggctcacca gctggatctg gcgtcccccc acctcgtagg
    46801 tgaagggctc catgagcacc tcttccccgc tccggagggg cttaaagtag tactcgccgg
    46861 aagccccggt attggcgtac tccacgcagg gttccagcat gacgctcccc gaccccttgt
    46921 tccagtatcc cacgaagcgc cctgtggcgt cgtggccggg tttccccacg tactccgcgt
    46981 ccttgccgtc gaaggcgttg ggctcccata cggtccagac ccccagaaag cggggatttg
    47041 tttccagaac ccgtcgcagg gcttcgaggg ccagctcccg gctggggtgc tccgaaccct
    47101 tcatggccgc cagcgtggcg gagagcgccc gggcggtgtc catggcttcg ttgaactccc
    47161 gccccaccct gtgggcgtat ccctccgcca gggcctgggc cttgtcgaag gcgtcttccg
    47221 tggcgagctg gcgcgagccc caggacacat agccgacgat cacgcccaga actcccacca
    47281 ccacccccag cacgcccacc agaatcttgg tacgaatacg ccacgaggac caccacattt
    47341 ttgtttcctc ctttttcgcg atgcgtagga ttttttcctc cgggctttcc ggatcccctg
    47401 gggacacgag gggcccggag ggctttcgac tcaatggtca atggtttttt cgggacgtcc
    47461 tgtccgaggg gcgctccgga gcttgccccc gtcgtctccc catcgcggcg aggggagcga
    47521 ggagaagaag cgcccccaga ggcacgatgc cgccatcgca gcctccccgg tgccccgcag
    47581 aaactccggt gggctcggaa tctgccccgg gaacgccgac gagggcttcg agggtgccca
    47641 ccaccaccga tccgtcgacg acgccgtcca ggtcgaagga gcccccgtcc tcaatgtcgc
    47701 aggtgagcgt ggcgtatccg gggggagtgt cctggcggtc ccaggcgttc tccggcatgt
    47761 cccagacttg ggaaacgcga tcccgcacga agaagatcca ccggtagatg acgtcgtcct
    47821 cctggagggt ctcggacgcc agggcgaggg tgagcgtgcc gtcgggggtg gcgtactcga
    47881 cgatgtccgc actgccgatg tccacgagaa cgaaggtggc ctcggcgtcg tcgccgaaga
    47941 tttccgcgat ctcctcgggg gcgaagcgca cgttcgacgg attcagttcc cccacggggg
    48001 ggagctcctc cgggggcacc ggcgtgggag aagtcagggg cacgcccccc gtcacctcgg
    48061 ggtccggctc cggcatgtcg gtgggggcgg ggatcggcgt gggagttggc cccggggcgg
    48121 agatcttcgc cgtcatgacg gctcccgggg cggagaactc ccgcagcgaa aagccgctgg
    48181 ccgtgccgtc gtggaatttg gagttcgggg agctggtggc ggagaaggtg ccgtcgccct
    48241 cgtagttcgg gtttccctgg taccagaggg tgtccttcgt gccgtcggat tccgcgtcct
    48301 tggtgaggtg gggcccgtcg gcctccttca ccatgttccc ctggtgtttc cccttgacgt
    48361 atttgttgaa atcgttcgcc gacggtgtcc ccacctcctc gtcgagatgg aggatgagca
    48421 ccccgccgtc gaagtcggca aaggagtctt cgaggccctc caagccctgg tcccatcccg
    48481 aggggcgtcg gacttccagc aggaagtact ccgtcttccg gtgcgatgtg cccaccagct
    48541 tcaccacgtc gtatttgctg ttgccgggct ctccgaacgt ggccaccccg ctggcggggg
    48601 tcgtggcggt ggcccacccc agatagaggc gcgaccaggc gtcgaggttc gtcggcgtgg
    48661 agccgctggt ctccttcgcc ttcttgcccc agcttccccc cgccatgatg gagaaggcgc
    48721 cgatgccgct attggtcttc gacgtgtcgt agaggtcggg aagggcacag agctgatgtc
    48781 caagctcgtg cgccacgacg ccgaagggca tgcgggtgcc tttggcgtag agttctccgt
    48841 tcatggacca gctggtgagc ttcttgccgc cggcgaagac gatgtgttcg tcgctttcgt
    48901 tcatccacga cttccacgcg tgggcccaca ccgacggctt gagcttggaa ccggactgct
    48961 catatccggc gatgatcatg tagaggcaca gttcgtcggg ggtgagggcc ccgtccttgt
    49021 tcgtgtcgaa ggaggcgaaa tccaccccca acgccgtggc cttttgggcc acggccgtga
    49081 cgaaggccac ctcgttcttg tgctgttcgt cgacgttgtc gtcgccgccg atgtcgatga
    49141 atccctgggg atgattccca tcgttcacat cccccgccgc gagggtcacg gagagcaccg
    49201 cggtgccccc ccgggccgag atgatttcga gttttccctt cgactggtcc ttatagtact
    49261 tggccagcga cgcggtggcg ccgaaaaaag ccccttccca atcccccgtc ttcgtcgtga
    49321 gcgcctggtt ccggaaggaa acgaggacca cgaggacgtt tctctttccc gaaaccggcc
    49381 tcgtgggggg atagccgaac tttttgtcga agtccggcgc cccgaggcgc cggggggcgg
    49441 ggatcatgtg cggtgcggtg cccgccggag gaggtccgaa gggcgagtag acggtgcccg
    49501 aaggcacgag gctgttgtcg cggagcgcgg cgtactccca atatccggtg gcggcgttcc
    49561 ggaccagggt gtggcccgag ggcgcttcgg tccagtgaaa gaactcgtcc ccccggtgga
    49621 acaccacccc cgaggtgcca tcgggctgta cgagggggat gggataggga caggccggca
    49681 ccccccaggc cgccggacag gcaaggacga ggagaaggca gagggaaaac gtggagcgca
    49741 gaagcgtggt tttcatggaa aatctcctcc ttttcgcggg gaacacgaga tccctgcgcc
    49801 gatggtggcg gcgaagagaa cctccggaag cgcctcagcc ccggaggtgg gttcttcgtc
    49861 gtgggggggg gccgggacgt cctagaacgt gccggaggaa ccgtgacgca gttctatgcg
    49921 gtccgttccc agagcagtcg ccccctttct ccccgggggc ggagcacccc ctggttcgcc
    49981 agccaggaaa gatgggccgc cacggtgagg tgggtgagca cgaaggacac gggggtgatc
    50041 tcgatgtcgt aggcctccgc cagggcctcc agcacctctt cccgggatcg ggggatggcg
    50101 cagagcaggg cgatctcctc cgacaggcgc tccgtgcggt gccgattgta ccgcaccaac
    50161 tcctttaaat ctttgcttgg ggtggcgtga ctcggcacga aaaattccgc ctccatggcc
    50221 tccagttgcc gaaaggtctg gagctgcccc gccacgtcgc agcacacggc gaagccgtgc
    50281 ttttcaagaa tctcttcggc gaagacgctg tctcccacga aggccacccc gtcgggggtg
    50341 cggaatcccg ccatgtcgag aaagtgcccc ggcagaggaa aggagaggag gccgctctct
    50401 cccacgggat cccccgcccg aaatcggtcc gtgacccggg aaggggaggc ctggaggaac
    50461 ttggagcaaa tttcccggaa cggcgtggcg ccccagagca acaggggctc cagggcgggg
    50521 tcgtcgatga gggccgcctc ccggggggtg gcggcgatgc ggcatcccgt gcgtttctgg
    50581 agcagggcgt tccccccgat gtggtccgca ttggaatggg tgtgggcgat gagttccagg
    50641 gtccacccct gccgttccag gagctggaaa attttccgcc ccagagattc gtcgttgccg
    50701 ctgtcgatga gcaccgcccg accttcccgg acgaccacgc ccacgttggt ggagcccggt
    50761 atgtaatagg tggaacctcc gcagggcact aattccacgg acggacatct cctttcggct
    50821 ttcggggtga atcccgaccc ttcccgagag gggacgcgaa cctcccattc ttcgaggtgc
    50881 atggtacttc atatcatgtc gctgggtcaa attatcggtt gatttttcta cgaagttctg
    50941 acccttcgta tcccctctgg ggggaggagg gttaccgggg ggccccggca atgtccctcg
    51001 gcagaagggg gataattctg ctatgatacg ggggaaactc tccggaggat cccataaaaa
    51061 ccatgggggg tcggggagga agggggaaag agcatggata aaagttcttc cggggacgag
    51121 cgtgctccga tcacgtcgcc cttggcctac ttggcggccc actacaccac ctcggatgac
    51181 ttggcgcttc aaaagcagct ttcggaggat ctggcccgtt tttcctccct cccggaagac
    51241 agcgccgacc ccgaggtgcg caaggagcgc cgggccatct gggagcgggc gtcctcgtct
    51301 tattggatcc tcgcctggga ggtgctggag agccggagca agggaagcgg tcgggccccg
    51361 gagttcctcg acttcgcccc ggaggagcgc ctcttgctcg atgcggcggt gcttgacccg
    51421 cgcctggccc ccttcaacgg ggaagatttg ctggcctccg ccgaggttcc tacccccctg
    51481 gatcgctatc aatactaccg tttttccgac ctgatggccg aggcctttgc tctgctcttc
    51541 gagaaggacc tcccccgccc ccgggggggg atgggcctcg acgagaagat cgcccacctg
    51601 gagcggcgcc tggaggggct cacccgccgc cgggggggga tgcaaaaggc cctcctgcgg
    51661 catgtctcct tcggggggct ggaggaggcc gaaaaggacc tgcgggagat ggaaaaacgc
    51721 ctcctggatc aggtggaggt ggatcagcgg gtgcagcgct ttcgtttcgc cgaggatgcg
    51781 gcgcagatcc gcatgcgcga atccaaggcc ctctacgacc ggacccgcca gcacctgatc
    51841 gaccttttag agcccctccg ggacggcttg ggggccatgg gcttcttggg agagttcgac
    51901 caatttctgg ccctgacgcc ggag
//
LOCUS       DAOOER010000008        51324 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_703_length_51324_cov_9.111334, whole genome shotgun sequence.
ACCESSION   DAOOER010000008 DAOOER010000000
VERSION     DAOOER010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 51324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51324
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_703_length_51324_cov_9.111334"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..>1310)
                     /locus_tag="PL162_02015"
     CDS             complement(80..>1310)
                     /locus_tag="PL162_02015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HQF90837.1"
                     /translation="RDDAMHMERVEEKTGWLLILHGRLDAAHGRNLEDEMEDALRQGA
                     HRVALDMGGVDFMSSAGLRILLRYHKALGALGGELVLRRTSEFVRDMLALVGMESLFS
                     ASEGQDGGDKGCPGPWGDGAPSYLLDPAGGFCLDLPGAGEERLLGPGVWGLGLGAFDD
                     APTSSGEILAAEGYALMHSPGGGAPDFMAAAGSFVPTVHFASGVILRGKPSHCFRFVE
                     ALPGIPLSLLARGALKALGGPGVALALVAETAGLVGARLGSSSPEGDDWRAVARRVGQ
                     EDFFAFPAVRDHLAYRPEKTYDRHLALVVGVATTNPVAPLGAQGRPLGEGGELWGHFH
                     GAVFPFRAIPQGRVELPGVLEKLFDAVAPVGLLHLLHDRRPISGAGESAFLSGALWAG
                     PLLGETGSLRGKKGREA"
     assembly_gap    1311..1410
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1411..3308)
                     /locus_tag="PL162_02020"
     CDS             complement(<1411..3308)
                     /locus_tag="PL162_02020"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQF90838.1"
                     /translation="MWKALKHLSLGLALIVAAGGVLLFSDQGGREGRRGPEHGPAEAL
                     HRKVRAGEKVRVALLKWVSSVTLDETLRGLLEGLAERGFVEGQGMELQVFCAEGDMPT
                     AVSMAQRAFGGDYDLVLTVSTPMLQVAASANKQGRRVHIFGAVTDPYAAEVGISREDH
                     RSHPAWLTGVGTFQPVRETLELSRRLYPGLTRIGTVMNPSEACSQSCYGVAREVCESL
                     GIELDVVTVDNSSAVYEAASALVSRGVQALVIGGDNTVESAFDSVVKAADGARIPVLG
                     YASMYAGRGALAGLGANYVDVGRVQGRLAGDIMKGLYPGDIPVENVMPLKLSLNRQVA
                     SRLRDPWELPPEVVQGASLVYGESGALEVSVDRSEGVPAIPAGRCFELYFLNYVESVP
                     MEETLRGFRRALDEAGLREGRDFNLEVGNAQGDMPTLSALVDHVRSRRVDLLLLTSTP
                     TLQAVLSKINDIPVVFGLVANPVLAGAGSSDVVHRPHITGISTEAPYVEGVRALKECL
                     PGAKTVGTLVNPSEVNCVYNLKQVEKALAAQGITCVSVAVSTPTEVSDGIQGLLAQRV
                     DAVLQVTGNLFFASFAPISKACLEARVPLFGMDAANALSGGAAVAVARDYAAGGEDMG
                     RVALRVLRG"
     gene            3566..4270
                     /locus_tag="PL162_02025"
     CDS             3566..4270
                     /locus_tag="PL162_02025"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HQF90839.1"
                     /translation="MAQEWLSLAEISRRTGIAESSLRRYARNFAPHLLQEQSGKRWVF
                     AEESVEVFDRIGTAYREGKTTEEVARMLETTPTVKSIDHTSPGGGPSVPAPAHQGAAP
                     EILPLSSASVALFFSTVQTAMERMAEALEAVQHRDQERVRLLEEKSTSQENLLEDQRL
                     RIKELEQKLHRVESLLFLAGDEKTTPLVVKDQLEKLQGDLAMVRQIQEKEAVVRILDQ
                     PTPRSPWWRRLLGLKP"
     gene            complement(4383..4760)
                     /locus_tag="PL162_02030"
     CDS             complement(4383..4760)
                     /locus_tag="PL162_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQF90840.1"
                     /translation="MAKILVVDDAPVVRLLLQNLLRPMEDKGVELLFADDGHEALSVI
                     KKERPTLVFLDIMMAGKSGYDVCETVKKVWGFDDVHIVFLTSRIHPSDRERGSRAGGE
                     GHIAKPFDPEEILAKVREFLPLD"
     gene            5256..6212
                     /locus_tag="PL162_02035"
     CDS             5256..6212
                     /locus_tag="PL162_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HQF90841.1"
                     /translation="MIKVMKSVAGNVIRELTLNTLEEGAWIHLLAPTRDELRSVAEAT
                     AIPFDLLAAPLDEEETSRVEVDDEAVLTLINLPKVSKEGGYDTIPAGIVVSERHIITI
                     CLEENDAIPQDVASAASRHFCTFKRTRFLFQILYRTASLYLRYIGQINRRSDDIEDRL
                     RASMKNQELFQMLDLQKGLTFFTASIRSNRAVVQKLLRLCANRQVSKFIKTREEDEDL
                     LEDVSVEFDQAFEMVQIYSNIMTNMMDAFASIISNNLNIVMKVLTLITIVMAIPSLVS
                     GLWGMNVPVPFSADPYGFYIVVAFVLVLVVASFVAFWKKRLF"
     gene            6340..6513
                     /locus_tag="PL162_02040"
     CDS             6340..6513
                     /locus_tag="PL162_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90842.1"
                     /translation="MTIQLWIISDDEAEERRIDHMEELVREILDESGMIYARGEARHK
                     DDSGKHIYLTDIQ"
     gene            6701..7975
                     /locus_tag="PL162_02045"
     CDS             6701..7975
                     /locus_tag="PL162_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQF90843.1"
                     /translation="MFAHAFQNLFRALKSRNYKLFFGGQAVSLTGYWMHRVAMSWLVY
                     RITGSPLALGIVDFMGQIPTLFLGFFAGTLLDRWDLRRVIIVCQSICMVHALLLATLT
                     LTGVVQYWHVVALSAVLGVVNAFELPARLSFVVHLVDDPEDLSNAIALNSTLFNGARL
                     VGPSLAGVVIAMLGEGLCFLTDGLSYLATLVALLSMRMTRSARAEAQPGKMLEETREG
                     WRYAMGFLPIRTILLAVASISFFGLPYLVLLPVFAGDILKGGPQTLGFLTAASGIGAL
                     FGSCRMALRSSPVGLSSVIARTMGLFGAAVATFAFSRVVPLSMVLMAPIGFSMVVTFI
                     ACNTLLQTLVDDDKRSRVMSLYNMAVIGIAPLGSLCAGWSASVVGAPLTQAMGGVASM
                     VTAFLLWRSLPSIQQSARSTMVAKGFLSSETA"
     gene            complement(8059..8655)
                     /locus_tag="PL162_02050"
     CDS             complement(8059..8655)
                     /locus_tag="PL162_02050"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HQF90844.1"
                     /translation="MKAMILYAHPNSQSFNHAVLNLVLEELDRQGIEGTVRDLYEMAF
                     NPVLGGGDLAAACIGEPLEDVRQEQEQLAAADLLVVIHPIWWGGMPAILKGYVDRVFS
                     QGFAYGYGPEGPVGLLKRKKVVVLNTTGGSEESCLQSGFDEAFRGMVDRGIFGFCGMD
                     VILHHFFFAVGCVEPERRNAMLEELRGILQEEVFSRLG"
     gene            complement(8802..9272)
                     /locus_tag="PL162_02055"
     CDS             complement(8802..9272)
                     /locus_tag="PL162_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90845.1"
                     /translation="MKRLQKGYGKFLSSDRRDFAPHTLVIVDPDPRFSAVDLFDAGPG
                     DLLMASWPPEISGAQEAFGAFLARILEKPSMENVVILDVSPAALSPDEGPLRDRGVLR
                     FEALRRMPSIQYAQVTRGLRILLLYFSVETGELEVFEHGTWEPFLSKEPWGGGL"
     gene            9429..>10828
                     /locus_tag="PL162_02060"
     CDS             9429..>10828
                     /locus_tag="PL162_02060"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HQF90846.1"
                     /translation="MRQWKRGSLVALALCGLMSGVATAGPVFDAITPFRDAERRGVDP
                     ALLSWGHQLLEIEFPYTWSEALEILEKAYPYAGRSRLETWLKYGAEFIMIDGEPRYFS
                     AVVNSFAHRHDDIMRRKNQEEGRAAEKFAAPLRSMAFSGPEESYPASTFQPFINPITY
                     LGTQTLRIPRSALSADGTLKLWFPLPIATGDQDAVRVLSVTPERWVRGLPTVNEELGQ
                     LYMEVPLQELSGDLDVRLTFTFRHWERRSVVNPAQVGTYDVASELYRRYTAPGIHTPL
                     TREIVDKAREIVGDEKNPWVAARKIYDYMLKHVRYSFTPHLSLGFTGTPEALYVLRNG
                     FGDCGAQSMLFSALCRAVGIPARTTGGYQMVPRHGGTHFWAEFYLPAYGWIPVDVTMA
                     DTASWADIPDEERISFENYCFGNMDPFRMVIQTDVEIPLQPRPESPVLLMAPSGVPMV
                     FQTPALVAPHFAVTPM"
     assembly_gap    10829..10928
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10929..11671)
                     /locus_tag="PL162_02065"
     CDS             complement(<10929..11671)
                     /locus_tag="PL162_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90847.1"
                     /translation="MRMLRRIFGVILAMAVGLGGAEGAARGVDDPLDPALATQIQRLM
                     EEWRQAPLIKPQRDIPGWPLYAGGDWSFQYPPGWGLRDANPQFLWVSDPSGAANFIFA
                     QVAQVPGMPSAEELRDLVFYRLVGQDPVRLVTMERKGLPGISVPGDDTGVSTVFIVRW
                     QAPSGELRLGSIQITILARGPLSTSYQCAVQSCPEDLWIGAWRDIFAPMFLSARFTIP
                     NYNSEVHPDSDGDGYADPVDQDPEDPNVH"
     gene            complement(11765..12892)
                     /locus_tag="PL162_02070"
     CDS             complement(11765..12892)
                     /locus_tag="PL162_02070"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HQF90848.1"
                     /translation="MVGEWEEWKGGGLPRGCVPCCPGCAPDMASAAEERDRKMAWLEK
                     SLALWKDRREDLRDGPEEQRWGYRDKVCLRAAWLPETGWALGLRRKDGLVAIPECPRH
                     SRRVRNMISWLRHHLPAAENFPLVYYVQAGGQGTLVLKSREDPDLSWMETAPDPGEAA
                     GLEGLWLHRHPGAGRRVFGRGSWRLLWGVPRSRDAQGLVYGPRSFQQVRPSLHLEALE
                     RAEAFLSPKPGDGVVDLYCGTGGSLRRWQERGAMALGVEMGGESLECAAVNVPGATLL
                     RGSCAQRLPQVERWLEAFAPERRLFYVNPPRLGLEPEVGAWMGSRGSFARGAYLSCSA
                     GTLARDLKVLEREGFAVVSLHPYDFFPGTPHVEVLVCLHRR"
     gene            13138..>13426
                     /locus_tag="PL162_02075"
     CDS             13138..>13426
                     /locus_tag="PL162_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90849.1"
                     /translation="MKDQADIMNQLKALEGKTGRGIISQLVTEETLGYMEKLNRDGVP
                     YAAVAEKLSASTGETVKPMQVMFAIQQYRKRHNMEPVRSSSGKPRGRKPQTP"
     assembly_gap    13427..13526
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13617..14771)
                     /locus_tag="PL162_02080"
     CDS             complement(13617..14771)
                     /locus_tag="PL162_02080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQF90850.1"
                     /translation="MRILVISFSDLRSDARVFRQLNALRRRHRVECVAYTDPGLPDVT
                     VHVAQGRVPSYREKLKELPLLALRRYDAYERCFAHGDLRLPGVMPGGYDVVLCNDANA
                     LPYGFSVCGDAPVVFDAHEYSPRQYEDVWIWRLFFRPYKEWLCRTYLPRLGGMVTVSE
                     GIAEAYARNFGVPQPQVVTNAADYVELSPSEMVPGKIRLVHHGICAPSRHIDRMLDTM
                     VLLDDRFSLDLFLVPGPPRRYFDAMVSRAEALPRVRIRPPVPLRDVVSTLREYDGGMY
                     ILPPSNFNHAHALPNKFFDFVQARLMVAIGPSPEMGRLVRQHQLGVVGEDFSPEALAA
                     RLRELTPEQVRVYKENSHRAARELSSETNMAVLERVLEEAARGRSAGAIW"
     gene            complement(14768..15871)
                     /gene="wecB"
                     /locus_tag="PL162_02085"
     CDS             complement(14768..15871)
                     /gene="wecB"
                     /locus_tag="PL162_02085"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HQF90851.1"
                     /translation="MGNMVMSIVGARPQFIKEAVVGAAARRLGAWRHVVVHSGQHYDV
                     NMSDIFFTQLAMPQPDYFLGIGSGSHGRQTADALARVEELLLAERPDLVLVYGDTNTT
                     VAGALAAAKLKIPVAHVEAGIRQAPRDMPEEINRVVTDHLADRLYCCSALARKNLEAE
                     GLGARGEVVGDVMYDLFLTLRLLFRPENMKKKFGVEERPYVLATLHRDFNVDTREPLT
                     ELLKGFAELRREYGLDVVLPVHPRTRQRLKDFALEDLTAGLRLVEPLGYVELMGLLEG
                     CTAVVTDSGGFQKEAYFAEKRALVMMPDTGWRELVEAGWHRLCAPDAQEMTRTFAALG
                     TSVPYPAGLYGDGTAGDRVVRALAAFLGARENR"
     gene            complement(15924..16685)
                     /locus_tag="PL162_02090"
     CDS             complement(15924..16685)
                     /locus_tag="PL162_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AglZ/HisF2 family acetamidino modification
                     protein"
                     /protein_id="HQF90852.1"
                     /translation="MIRTRVIPCLLLRGDGLYKSVKFSDYTYIGDPLNAVRIFNEKEC
                     HELVFLDITATREGRGPRFDLVKDIAGECFMPFSYGGGVHTMADIAALLRLGVEKVVL
                     NTAAFENPAFVREAAREYGSSTMVISLDVKKNFWGSYRVCTHGGQKVRDVDPVTQAQK
                     MEECGAGEIFLTSVDRDGTQQGYDISLIQSVASAVGVPVVACGGAGKVEDFGAAVHEG
                     GASAVAAGSLFVFYGKRRAVLINFPSDEELRPFLA"
     gene            complement(16682..17317)
                     /gene="hisH"
                     /locus_tag="PL162_02095"
     CDS             complement(16682..17317)
                     /gene="hisH"
                     /locus_tag="PL162_02095"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HQF90853.1"
                     /translation="MEKILIAIVDYGTGNVGSLGNMLHKIGQESELASTPDVLERADA
                     ILLPGVGAFDRGMGNLQASGLLETLTRRVRDEGVPVLGICLGMQLLARSSEEGTLPGL
                     GWIDAVCRKFSFPPEDRQHKVPHMGWNAVIPRSGEALFAGMEPGALFYFVHSYHVVCA
                     DPGDVLGVSEYGETFTSAVRRDNVFGVQFHPEKSLRFGMRLLRNFCEGVGR"
     gene            complement(17302..18471)
                     /locus_tag="PL162_02100"
     CDS             complement(17302..18471)
                     /locus_tag="PL162_02100"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQF90854.1"
                     /translation="MTQGRVVHVFLSNGTRESRFAKEAAVVLEEGLFDEVVFVGLHDE
                     GLPLGESFAPGVRVDRMRLWSRKLRWGVVGHLCKYVEWVVRALHAVRRNRPRVVHCHS
                     LPALPVGVAAKMLWGVPVLYDAHELETEMAAFSGGKKAWAKRLERWLIGRVDAFFVVG
                     DAIADWYVRAYGVSRPMVVRNIPRGVSLVRKEAWNLRQRLSLPPEATLFLFLGGMGPH
                     RGVEEILKAFEGVRGGRHAVFVGDGPLKDVVCRRANEVSNVHYLPPVPPEEVLSVAQG
                     ADVGLCLFEKNTLNNLYSLPNKIFEYLFAPIPLIIADLPEQRRIVEAFSCGWIWNGNP
                     EDLVHLVNQLDPAGNDYAEKRAGAERAARELDWDKEVRSMVEAYGRLGGGAWKRS"
     gene            complement(18468..19574)
                     /locus_tag="PL162_02105"
     CDS             complement(18468..19574)
                     /locus_tag="PL162_02105"
                     /inference="COORDINATES: protein motif:HMM:TIGR03573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HQF90855.1"
                     /translation="MTYRQCTRCIMDTTDPEITFDDRGVCNHCRRFDEELRPLWRPNA
                     EGRQRLEKILETVRREGQGKEYDCIIGLSGGVDSSYMAYQVVQMGLRPLVMHVDGGWN
                     SEVAVRNVESLCRKLKLDLQTFVVDWEEMRDLQVAFLRSGVANQDVPQDHAFFAALYA
                     FAVRNGIRYVLSGSNYATEGILPSSWGYDAMDARQLRSIHRRFGEIPLRNYPTVSFYQ
                     YYFHYPYVKKMTVLKPLNFLPYVREEAVRELQREFGWKHYGGKHCESRFTKFFQYYFL
                     PRRFGYEKRRAHLASLVVSGQMTRDQALAEMARPVYQEGELGADREYIVKKLRLRDED
                     LEQFLTMPHKTFRDYDSHFGMVQTLRTLKGLVRR"
     gene            complement(19571..20158)
                     /locus_tag="PL162_02110"
     CDS             complement(19571..20158)
                     /locus_tag="PL162_02110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQF90856.1"
                     /translation="MKKIIKTLVFKISMIVLPFFFQRSYLSGRWFCWPVLDGIFWAWR
                     GVWWQKIFGFNRRIPWPVSPLCTVGDPRMVRFHPDNLDNFQSPGCYFQCGGPLSIGYG
                     VYIAPNVGLINANHDPWNLDRHVEGSPITLGDHCWIGMNAVILPGVELGERTVVGAGS
                     VVTKSFPEGHCVIAGNPARILRRLEKPKEEREGTS"
     gene            complement(20161..21612)
                     /locus_tag="PL162_02115"
     CDS             complement(20161..21612)
                     /locus_tag="PL162_02115"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HQF90857.1"
                     /translation="MLKNLRASLEGKLQRWPFFRDVVFVGGGAFLGQAVALVCAPVLS
                     RLYAPGDFGVLAVYAAFLSAMTAFSGLGLHLAIPLTETRSRAWRVAGAAFLAHGFMAV
                     VFSVLLFFFQGALRREYPFFSDLLLMGCLLVGFLLSGIYDIFSYLGIREKAFATIART
                     RISQGVWGALSGVGLGVLGVRPLGLVVSQVCNMAAGISVLRRRLVPPGSVRELFSREL
                     WDDVVAFRRFPLFQPWTNLSAILSSQIVPILFARFGGVAAAGILFFNMKVLQVPMMFV
                     GNAVAQVFFQRGSELSREGHLGVWVAKVFRGLMLVGLYPVAVTVLLGPQLFPVIFGAA
                     WKEAGALLPVMGPWFFAQFLTSPLSSVMYIRGQQRCDFICSLVQLCLRITSISLGLMY
                     FDMQGAIVLFSGANVFFYISYLFLVLKFSHVSFSDILNSIKESFLFLVTCLVPLAPLW
                     YFSEITICIITLPLMSAGMWVFLLKRKLKELKQ"
     gene            complement(21606..22607)
                     /locus_tag="PL162_02120"
     CDS             complement(21606..22607)
                     /locus_tag="PL162_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQF90858.1"
                     /translation="MYRIALLGCGRISKNHLEAVAQMENARIVACGDIVRSRAEEAAR
                     KVGEGCQAYDNYAKMLQEVECDLVTLCTPSGLHPRHAAQAARAGRHVLSEKPLGCRLE
                     DCDEAIQACDDAGVQLFVVKQNRFNQTVQLARRALEAGRFGRIHMVMANVFWQRPQDY
                     YDQARWRGTWELDGGCLSNQAAHYVDLVQWFGGSVAEVSAFGATLERRIEAEDTTVVN
                     LRFRSGALGSINVTTLAYPKNLEGSLTLLGERGTVRIGGVALNKMEHWEFADSHPMDE
                     EVAQANTNPTSVYGFGHLPYYRHVLNVLDGKEEALSDGREGRKTVAIIEAAYGLRKC"
     gene            complement(22628..23947)
                     /locus_tag="PL162_02125"
     CDS             complement(22628..23947)
                     /locus_tag="PL162_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HQF90859.1"
                     /translation="MTLLEKIKERQARVGVLGLGYVGLPLAVEKAKAGFRVLGFDIQK
                     DKVARVNRGENYIGDVVDEELTRLVASGHIEASTDFDRLRECDVVAICVPTPLDRYKQ
                     PDLSYVTNSGKEVAARLHPGMLVVLESTTYPGTTEEVLLPLFQASGLKVGQDFHLAFS
                     PERVDPGNIRFKTKNTPKVVGGCTPACAEVARALYEAVLEAEVFVVSSPREAEMAKIL
                     ENTFRIVNCALANEMAVVCRKLGINLWEVIAAAATKPFGFMPFYPGPGVGGHCIPIDP
                     FYLTYKAREVDYHTRLIELAGEINDAMPEYVVSRLQDLLNDQSKALKGSHIVLLGLAY
                     KGDIDDLRESPALKVWELLEHKGARVTPVDPYCPSVRWNGETRATAELSADLLRGADA
                     VVVASGHRHRVDYALLVEHARLILDAKNVVAPALKKSPADIAQLALL"
     gene            complement(23971..24723)
                     /locus_tag="PL162_02130"
     CDS             complement(23971..24723)
                     /locus_tag="PL162_02130"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQF90860.1"
                     /translation="MFMRAAQVASTARIGQHVVIEDDVHIGEHAEIGHGVILRQGVRL
                     GEGCRIGDGAILGKLPARASLSATTGEPVELPPLVLGKEVTVGAGCVLYRGAILGDRV
                     FLGDLATVREDVTIGELSIVGRGATVENKTTVGRRVKIESNAYVTALSVVEDYCFIAP
                     EVTFTNDNFLGRTEERKKHFGGPRVRRGARIGANATLLPGLEIGEDALVAAGSVVTRD
                     VPPGVVVAGNPARVVRDVAPEQRLENQTYYEG"
     gene            complement(24714..25304)
                     /locus_tag="PL162_02135"
     CDS             complement(24714..25304)
                     /locus_tag="PL162_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HQF90861.1"
                     /translation="MIDTLITSKTRIKLLLKFFLNPDVRGHLRGLADEFGESTNAVRT
                     ELNRLAEAGLLLVADEGRRKLYGANSAHPLFPEIRSIVAKTMGMDQVVEHVVRRLGNV
                     ELAFVTGDYARGRDSGLIDLVLVGDVETTYLQDLVARVETLTERKLRPLVLSRGELER
                     LQEKFVAEGALVIWSDGHAGDPSFVDLTQEVRSSCS"
     gene            25507..25809
                     /locus_tag="PL162_02140"
     CDS             25507..25809
                     /locus_tag="PL162_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HQF90862.1"
                     /translation="MSIVVAELVVSPLGTGSPSISAYVADVERLLETFALKRLLTPMG
                     TILEGELDEVLRAVRAVHEAPFARGALRVSTSLRIDERRDKTLSMEGKVRAVEEKL"
     gene            complement(25905..26408)
                     /locus_tag="PL162_02145"
     CDS             complement(25905..26408)
                     /locus_tag="PL162_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HQF90863.1"
                     /translation="MKPALLVIDVQKAFFHISGETRKSLEQAVAQINAVVPLFRKKNL
                     PVVWIQHQDDEDGVIPGTEGFEMADSFDVRPGDFRVVKTAGNAFHGTNLEEELRRLGV
                     DAPVLTGFCAEYCVLSTFRGARDRGLAPMILRGATASVAPENIPFVERVNEVLSLGVL
                     VKMLENC"
     gene            26642..27829
                     /locus_tag="PL162_02150"
     CDS             26642..27829
                     /locus_tag="PL162_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HQF90864.1"
                     /translation="MQERRYAHRMGNMRASDIRELLKMSTSPGVISLAGGLPAPELFP
                     TQDIADASCKVLHEMGQVALQYTTTEGYAPLREKIAARMNRLFGASATADNVLIVSGS
                     QQGLDFAGKLLIDDDDVLVCECPTYVGAISAFRAYLPRFEEAPTDDHGLIPEELAKIL
                     AREPRVKAIYVIPDFQNPSGRCWSLERRQAFMELVSQYNVVVLEDNPYGELRYEGENL
                     PSLKSMDKKGQVVMFGTFSKILAPGMRIAWILAEKDLVEKFVLIKQGADLQTSTISQM
                     EINAYLEDNDIDVHVEKIKKLYRARRDYMVQSIQRHFPAEVTMTYPTGGLFLWCTLPE
                     SCNARELLKIALEKKVAFVPGGGFFPNGGHENTMRLNFSNMPEDRIEEGISRLGAAIK
                     AFL"
     gene            complement(27978..28889)
                     /locus_tag="PL162_02155"
     CDS             complement(27978..28889)
                     /locus_tag="PL162_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HQF90865.1"
                     /translation="MNKRCQRVLVALVMVWALAGAAFGGVVVYQTDFGLKDGAVSAMK
                     GVALGVDSELKLYDLTHEIPPYNIWEGAYRLYQTVAYWPEGTVFVSVVDPGVGTERRS
                     LVLRTKSGHIIVTPDNGTVTLVAESLGVAEVRQIDEQKHRLPGSDQSYTFFGRDLYSF
                     VAAQLAGGKLRVEDVGPALDPAQLVKIAYQKAESREGALFGTIPVLDVEYGNVWTNIG
                     KELFDAFQSKFGDEFDVVISKEGKEVFSGRIPYAETFGSVPEGKPLLYFNSLMNISLA
                     INMDNFAEKNGVSSGPEWTVKVAPAAK"
     gene            29287..31155
                     /locus_tag="PL162_02160"
     CDS             29287..31155
                     /locus_tag="PL162_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQF90866.1"
                     /translation="MSRKDIMLGNEAIARGLVEAGCEVAASYPGTPSSEILPAVAHWA
                     EELGTRTVVEWGANEKVALEYAIGASFARKRSCAIMKQVGLNVAMDPFMSIAANEMDG
                     GVLLVVADEPGCYSSQTDQDSRALALFAKIPCFDPCDAVEAKAMVFDAYEISEKYRVL
                     TMLRPTGRVNHCRQAVECGDGVIPGRPLEFRRMPEGHRILMPMTMRGALERHNRRFEE
                     LRREFGTAFGKYNFEVPAQGPARLGIIACGLCYAVVRDVLKALGRTDVALLKIGTPVP
                     LPLDLVQDFIGRHDSVLILEETFPVVEQQLLDRTKVQGRWNGWVPMEWELSPEVITSI
                     ILRALGEPAALPAWEATLEEARKALQLGRRGPRLCPGCGHRPSYLAIRKAYPDCILCS
                     DIGCYGLAIAQKAVDTNVCMGGSITVASGIAMAFRAEGKPASRPIFATLGDSTFFHMG
                     LPGLASAVYNKHPFVLVILDNGITAMTGGQGHPGVGGKLRPGEKSGSVDIEGAVRGCG
                     VTHLVVQEPYDVSGSVRVLKEAWEHARTAEEPAVVILRHPCITMLKAPPTPRPVRVDP
                     DKCTGCRYCLTHFNCPGIGWDENTKKTFIDRRFCVSCGVCVDACPRDAIVFTGEEA"
     gene            31157..31714
                     /locus_tag="PL162_02165"
     CDS             31157..31714
                     /locus_tag="PL162_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HQF90867.1"
                     /translation="MKYIVAGIGGQGILFATKVLGHIALGKGLPVIGSEVHGMSQRGG
                     SVISHFKIGDYQSPLIASGDADIFLAFDQSEALRNYHFLREGGQAVINVERPAELAHP
                     RLQDYFRQRRITLHALDGLGLIREHLGGKFLFLNVLILGALCGAGVGNFSRDEMRQAV
                     GELAPARFREENLKGFDLGYGALAK"
     gene            31743..32831
                     /gene="buk"
                     /locus_tag="PL162_02170"
     CDS             31743..32831
                     /gene="buk"
                     /locus_tag="PL162_02170"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HQF90868.1"
                     /translation="MTTATPQAPLRVLAINPGATSTKVGVFEGETPLLKKSVEHSTAD
                     LAKFPRVADQYNYRFELVEAALRDAGVALDSLDAVVGRGGLMNPVPGGVYEVNDAMVE
                     DLRKAEHGEHPSNLGALLAREFARKAEVRAFIVDPVATDEMEPVARISGLAGLDRTGF
                     HHALNHRAVAKKVAREMGKRYEDLDLVVAHLGTGITVAAHRHGKSVDVCDARSEGPFS
                     MDRAGGLPAYSLMNLCYSGKYTHKEMRELLIARGGMFSYLDTKDLRQVERMAQEGHEQ
                     AALLIDALAYQVAKEIGAQATVLEGHVDRIILTGGMANSALLVEKISRRVAFIAPLAL
                     VPGEEELEALSAGVQRVLRGEETEKIYR"
     gene            33041..36067
                     /locus_tag="PL162_02175"
     CDS             33041..36067
                     /locus_tag="PL162_02175"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQF90869.1"
                     /translation="MPLVLVMDHNAPTLIRSLEREGFKALIASSEERVLAAARSADPS
                     PDIALIDLGATGGGFALAEKIRRLRPIPVVLLANAPERPLLDKHRNGEIAGWACRLGG
                     PALVAATVDAALKSSLAATRLSSESQDSLSAALLSHAAEGIVGYDQNLRYRSWNAFME
                     KFTELSAREVIGKRPAELFPPGAEGPMEGLRERALKGETVSFPPQPFHVPQTGRYGWI
                     SGVYTPHRDGQGHIIGVLGFFKDVTSERRSRELVELLLAEADAGLWEWIPATDALTVN
                     DRWEALLGYAPGELALLSPTWKSIAHPEDVPPMQDSLRRHLAGETPIYEGPLRLRAKD
                     GEWVWVLARGKATEWDVQKKPCRVSGTIVNISSCKVSEAHLRAQRDLALALASVNSLP
                     QALELCLDAALGAAELDAGALYFVEHDGLHLVHSLGLAEEVSLERRHCPPESELHRLV
                     IAGKGVFVPPRTLGLSPNALLQERDGFKALAIVPFGAQGKILGAFLLGSRTRETWTGK
                     IPSLLGTMAAQIGPAIAQLRAEESLRKSQEELQGLFNALEDFLFIFDDRGQVVWANSL
                     VFSRLEYAEEELLGQPLSRVHAPQDASEAPEVFDKILRGEINLCTLDLLPRSGDPIPV
                     EARFSRGLWGGKGAFFGVSRDISSRRQAERALHQANEALRQGLDQLRQRHRELAILND
                     LSTLLQTCLDMDEAKSIVAQQMALLFSEESGALYMAVPGKNILERTTVWGPAPPEEFL
                     ISMEDCWGLRRGKSHLVPRGASATRCRHTPPREDTSSLCIPVAVQGENLGLLHLRRDS
                     AEFSESGLQLAQTLADTMGLALANLKLRETLRQQSIRDILTQLFNRRYMEETLDREIF
                     RAQRTERPLGIIMLDIDRFKDINDRFGHAVGDEVLRQLAHLLQNSVRSGDVACRYGGE
                     EFVLILPEASLAHTAARAEELRQAARAIAIPAPGAEGETLAISLGVAAFPDHGPRGEN
                     LLKASDDALYSAKTSGRDRVVVAPIPLQGGSSGIRA"
     gene            complement(36205..38304)
                     /locus_tag="PL162_02180"
     CDS             complement(36205..38304)
                     /locus_tag="PL162_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQF90870.1"
                     /translation="MMAKKGLSLKVKVTGGVLILLLALAAILGGVSYLSQKDRIEAEV
                     LRVAEMDRSLFEAVWQDGAHRALTLADGVVALPEVRRAFASRDREGLFALVDPLWKAA
                     NGRHGIGQMQFHLPDVTSFLRMNSKKFGDSLLFRTGLVAVLRQEKPFTGPELGQFGFY
                     LRGILPVMEEERLIGSFEVGIELKQVLRRVAEIAGASFSVFAPAADVKKMEFAASFDV
                     AGFSLVATTEEGASPERFPAELFGGIMEKRDSYVWHGEDVLLASPFVNSFGEVVGAVV
                     VRASARDGLAALADLRRWALAAVGGMVLLSAWGAVFFLNRSLMVPLRGLLAMTRELAG
                     GSADLTRRLPVTTQDEIGEVATNFNAFLDTLSSLVGRVKGNAKELQRRTDDVSEASRG
                     LAAMAETLSSTSTALRSVVGENSSAVESINAGMEEVAAGTQTAAEASGKAAERADGAR
                     SFAHQAAGSVSQLGEAAQEMARATELTSAKVNEVQDFALRIGELVSSIGQIADQTNLL
                     ALNAAIEAARAGEHGRGFAVVAEEVRKLAEESNRTAGSIADLVGRIRGAVGETVQSMA
                     TTGTSVQNAVELVRGVRSGLDRLLEMVAGIADAVQDIAAVSEEQSASAEEVAASTDRI
                     SSFTNRAFGEAERLATIAAESSSSVARVAENAGILYERASELGELVERFTVEEDAPQE
                     PLPSQGKRVLSHSRGRG"
     gene            complement(38432..39418)
                     /locus_tag="PL162_02185"
     CDS             complement(38432..39418)
                     /locus_tag="PL162_02185"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HQF90871.1"
                     /translation="MAKGGLSLRTRMMVGVLLLTLMGISIVWGRNFLALREERIEAEL
                     RQVAENDQEVFDAVCRDEARRSLILADRIRAFPGVIQVFASRDRGALAALVDPLWKMA
                     NESYGVGQMQFHLPNVTSFLRMNSKKFGDSLLFRTGLVAVLTQGKTFTGPELGIFGFY
                     LRGILPVMEEERLIGSFEVGVELRPVIHRVAEATEASFSVFAPTDDVRKMEFAASFDV
                     GGFSLVATTEEDASPERFPEELFKGIMEKDSRYLRREGEGMRASPLMNSFGEVVGAVV
                     VRVDARSAQEAIAEGRSGARAAAAGGGALCALVGGYLLRKRPSSPRAKARRP"
     gene            39638..40687
                     /gene="dctP"
                     /locus_tag="PL162_02190"
     CDS             39638..40687
                     /gene="dctP"
                     /locus_tag="PL162_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HQF90872.1"
                     /translation="MRFKVLALMCLGLFLAVPAAAESITVKICYNGPPQPGNNAVHAF
                     VLNFQKFLEEGTAGRVKLELYPDSQLGSEEERMELLSRKGMNQPILNVASFAGVAPVF
                     PEIYASSLPFLFDNHRAAHIFFDEGLWWKKAQEAFRERTGAVLLEAVEEGGFLAFTNA
                     KREIRTPNDFEGLKFRGMDEGQIILYKSFGASGTPIPWTELYLALRTGVVDGQMNPAM
                     YVLMGSLYEVQKYMTLANIQYSDQFLVANGDLFDSLSPEDQKALVDAAKRANEISRAE
                     VEAADAAQVEQLRAKGMQIYTPSREELAVFRQRAQKPYLAWLETKVGPEELTLALDSA
                     KAANDRAAGVCPLRN"
     assembly_gap    40713..40812
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            40942..41445
                     /locus_tag="PL162_02195"
     CDS             40942..41445
                     /locus_tag="PL162_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HQF90873.1"
                     /translation="MTAMADRLQRISRTLERVSAVGAGALLVVNVADVLLGIVCRYVF
                     RISLVWTEEVARYSLVWLALLGAGCAAARGDHMALDFLVPRLPRAPRLVVEVLRLAFV
                     LAVLLALAVLGWKNLAGTWEVKTMALGVPKAALLLALPVGMLLLGLQLFLGALLPPEN
                     SERTEGP"
     gene            41445..42734
                     /locus_tag="PL162_02200"
     CDS             41445..42734
                     /locus_tag="PL162_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HQF90874.1"
                     /translation="MWCALLGAFFLQMLLGIPLFVGLVATAFLGVASLGNESLFRVIP
                     QQFFGGMDVFSLLAIPFFLLTGTLMNRAGLTDRLMDFCRLLVGSARGGLGYVNVVGGI
                     ILAGVNGSAAADASALGSLLIPAMEKEGFPRAYAAGLTAGSALIGPIIPPSIFMVIYA
                     SMTNASIGGLFAAGVVPGFLLGGAFLAMNALWSARYRFPFSDGAAVRARKGEILKRST
                     GALVAPLLIIGGIVAGVVTPTESGALAAAYCAAVGFFGTRQLTLRGLGEALVETVRLT
                     SAIFLIMGAATSVGWLLKWERVPQTVAAALAGWGLLDTPLLLLLTLSALTFVIGMFME
                     EVAVLTLLTPIFAPLAARAGIDPLHFGVVMTLNVTIALITPPVGACNYIVAAVGRVPL
                     GELFREIWPFIGVAVGTLLLLILCPPLTTALPRALGL"
     gene            42901..43800
                     /locus_tag="PL162_02205"
     CDS             42901..43800
                     /locus_tag="PL162_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQF90875.1"
                     /translation="MIIVLCLLTAVCWAASPLLLMVGMRSANLYEVNFIRALGFTGVC
                     GAWLLATDPSAMIWHHPLSLLGAVGANVMLGNILGDLLYFQSLSDIGVSRAAAITSTY
                     PLVAAGISAFWLKEPLTMPLILGTASIVGGLILLRLEGTSADAQVRAHPVRGFLWALM
                     AGIFWGMGIPITKWLVASQQVPPSTVNLWRSLVLLLFSGALWLWPRRGTPLGSRFRRL
                     GTIPPAGWVTILASGGVSLGVAGFIFISVLRFAPASMVTPLTSVNPLLSTLFAVCFMG
                     ERLRRIQWIGSVLVVVGSAAVGM"
     gene            complement(43989..44459)
                     /locus_tag="PL162_02210"
     CDS             complement(43989..44459)
                     /locus_tag="PL162_02210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90876.1"
                     /translation="MRKTVVSVVAVLALVAFASMAAAAAMPDFSGTWKWEKDGKTLVL
                     ELAQKVQKEKGKPPILVKGSHKATAEDGAKVDSSNGEISIFSDNFVDGAILVKIRSYA
                     CDAEGRAMMRLEGPKKDIMLFQIVSPPEGEHFIPEGNVYLKKVAPAPKATKKGK"
     gene            44690..45460
                     /locus_tag="PL162_02215"
     CDS             44690..45460
                     /locus_tag="PL162_02215"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQF90877.1"
                     /translation="MKVKANRQVLKGALGIGAALALGAAILVASTPKEAVYVLCLHHV
                     APVPGEDGGMYVSPGEFASLLARLRQGGAAFLSLEEFRRWIEGTGPIPPVSVLLTFDD
                     GYEDNASELLPILRSFDVPAAVFVVGANVGTPGRLSAPQMRAMAQSGLVAFGSHTQHH
                     RDLRGLSAAQLTEEFAGSRAFLEGLLEVSVDVLSYPNGRNGPAIRRMAAKSGYALAFG
                     TEGAPCTRSSDPMNLPRYTPGAFSRALGWRLAIRRLLE"
     gene            complement(45468..46454)
                     /locus_tag="PL162_02220"
     CDS             complement(45468..46454)
                     /locus_tag="PL162_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90878.1"
                     /translation="MLSLIRSGPHFLMLRGEGEALRSFLIHNFEATFGLVDPVDQSRE
                     DQTVLHVEDSSGGDLSVGLCNASPAEIFCALLNVKGDSGLKESRLLPRWIFLRSFGKG
                     SSVLEDIRRELGGTVASLDALFEWRLRSGTVVGCTSRPLNRPVLWQDLHPEVLVLDLP
                     FDLAYRRLRPRAFELFSQSLGDATWGEVQVQIYDKYDLYPEHIRRVELVLDALEMGFP
                     FSEGWTREAARMLLVVRVYRIRLLTFLAPSVVKEFLMGLEYDATGERILDMDLYCGRE
                     KIGWGPLGRERGLSRDELGRRAREKLWRALPADVVRELEELERRVRDASGME"
     gene            complement(46555..47931)
                     /locus_tag="PL162_02225"
     CDS             complement(46555..47931)
                     /locus_tag="PL162_02225"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQF90879.1"
                     /translation="MSRFFLRLLLWFWFTLAVALLAFQMVTGTLAPELPAERWERGVF
                     QAATLFGQGLGDLWRQHGAQAARARLDLLETTVGIRGFLFDATGEERLGRSVSDMVRR
                     EAQKTLTSRSPSRSARGTQTLLVVPLENPPGVLVLQAEWPSWQSALPALILRVGAVLL
                     VVGVMAWQLAHHISRPLLQLREAVRRFASGDMRARAPRQAYRHKDEIGALMEDFNAMA
                     DRMQGLLLSQRRLLGDVSHELRSPLTRASLALALAQKQLPEGAAPLDRVERELHRLEE
                     LVSSLLSLSSLNMMNRPARNDLVDLSTLVRQVAEDGAFEAESGGKQVEPHLTPGIWVR
                     GDESLLRSALENVVRNALRYAPGGTSVGIDLRREPSGARPVRLAVRDWGPGVPDGDLG
                     SIFRPFVRSERARERTGEGGAGLGLAIAARAVKLHRGIIRAENPSGGGLRVEMALPAA
                     DPPEDFEA"
     gene            complement(47913..48602)
                     /locus_tag="PL162_02230"
     CDS             complement(47913..48602)
                     /locus_tag="PL162_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQF90880.1"
                     /translation="MRKILLLDDDVELCELLREYLASENFAVEFAHDGAGGLVRALEK
                     DFDMMLLDVMLPGRNGFEVLRELRGRSTMPVLMLTARGDDVDRIVGLEMGADDYLPKP
                     FNPRELVARIRAVLRRHGDRGESMTRLAVGDVVLDSSSRSVRCDKKEVDLTGVEFKLL
                     EALMRSPGRVVPRDRLARAALDRPLEPFDRSLDVHVSNLRRKLGPFADGSPRIRTLRG
                     EGYLFACPGFF"
     gene            complement(48574..49053)
                     /locus_tag="PL162_02235"
     CDS             complement(48574..49053)
                     /locus_tag="PL162_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HQF90881.1"
                     /translation="MDEEGFPEREALRRAGLRRTPFREALLGLLRRAEGPLSHGELLA
                     RMPHGDRVTLYRALEALERSGVVHRIQGTDGVWRFRAHVAETPRCPGGHPHFLCTACG
                     RMWCLEGQPWPTVAVPPGARVTDKQLVVSGLCASCLAEEPSSRKEGVENAEDPLAGR"
     assembly_gap    49072..49171
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <49172..49702
                     /gene="modA"
                     /locus_tag="PL162_02240"
     CDS             <49172..49702
                     /gene="modA"
                     /locus_tag="PL162_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HQF90882.1"
                     /translation="EQGAPYPLFVSANEKWASYLEEKGLLREKRPVAQSALVLWWPQD
                     TAPSLDLLKDPQKRVAVANPDVAPLGVAARDYLQSEGLYDAMEKEKRLILTSDILGVA
                     LAVKSHGADLGFLAQGTAKKLGGSWASLPGKPQQYVGGLTAPESSEVIRDFWSFVTGP
                     EGGRILEEEGFEVARP"
     gene            49932..>51324
                     /locus_tag="PL162_02245"
     CDS             49932..>51324
                     /locus_tag="PL162_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HQF90883.1"
                     /translation="MITPDKIRNVAIVAHIDHGKTTLIDAIFRATRTFRDNARVQERV
                     MDSGELERERGITIRAKHCTVFWQDYQINIIDTPGHADFSGEVERVLSMVDSVLLLVD
                     AAEGPMPQTRYVLLRALRMGFRPLVIINKADRPNANPQDALNRTFDLFLDLGANEHQA
                     DFSVLYGSALQGWMVRHMEKDPREGLDPLFETIVERVPPPQVDPQAPFRMQVSTLAWS
                     DYIGRIGCGKILSGKISLGDPFSRIATHWTDEGHTAWEVTSTKMEKVAHLWATRGLDR
                     TAVDEAQAGDIVWIAGPQEIGIGDTFAAPDFPQAALVPLEIEEPTVSMFFLVNTGPLA
                     GQEGKAVTLRQVRDRLERELKSNVSLRMEDLGRADGVKVSGRGELHLAILIEEMRREG
                     MEFCVSRPEVITKRDAEGNLTEPIEQLIVDVPEEYQGVVIEKLSRRKGELVGVFPTGP
                     STVRLEFTIPTRGL"
BASE COUNT         9577 a        17402 c        14955 g         8890 t
ORIGIN      
        1 aaaaggcgaa aggtcacgaa gatccccagg gccatgaggg ccagaaggag ccccaaagtc
       61 aaagccccca agagcagggt catgcctctc ttcctttctt ccctcggagg gaccccgtct
      121 cccccaaaag gggccccgcc cagagggcgc cactcaggaa ggcgctctcc cccgccccgg
      181 aaatggggcg ccggtcgtgc aacaggtgca aaagccccac gggagccacc gcgtcaaaga
      241 gtttttccag gaccccgggc agctccaccc gcccctgagg aatggcccga aaggggaaca
      301 cggcgccgtg aaagtgtccc cagagctcgc ccccctcccc cagggggcgc ccctgggccc
      361 ccaagggggc gacgggattc gtcgtcgcca cccccaccac cagcgccagg tgacgatcat
      421 aggtcttttc cgggcgatag gccaggtgat cccgtacggc gggaaaggca aaaaagtcct
      481 cctgccccac ccgacgagcc acggcgcgcc aatcatcccc ttcggggctc gaagacccca
      541 ggcgggcccc cacaagcccc gccgtttcgg ccaccagggc cagggccacc cccggacccc
      601 ccagggcctt cagagccccc cgggccagga gagagagggg aatgcccggt agcgcttcga
      661 caaagcgaaa gcagtgcgag ggcttgcccc gcaggattac ccccgaggcg aagtgcaccg
      721 tgggcacgaa gctccccgct gcggccatga agtccggagc cccgcctccg ggggaatgca
      781 tgagggcgta gccctccgcc gccaggatct ccccggagga ggtcggggcg tcgtcgaagg
      841 cccccaggcc cagtccccag accccagggc ccaaaagcct ctcctccccg gctccgggaa
      901 ggtcgaggca aaagcccccg gccggatcca agagatacga aggggcccca tctccccagg
      961 gccccggaca tcctttgtcc cctccgtctt ggccttcgga ggcggaaaag agagactcca
     1021 ttcccaccaa agccagcatg tcgcgcacga actccgaggt ccggcggagg accaactctc
     1081 ccccgagggc ccccaaggcc ttatgatagc ggagcaagat cctcaacccc gccgagctca
     1141 taaaatccac accccccatg tccagggcta cccggtgggc cccctggcgc agggcgtctt
     1201 ccatctcatc ttccaggttg cggccgtggg ccgcatccag gcggccgtgg aggatcaaaa
     1261 gccatcccgt tttttcctcg acacgctcca tgtgcatggc atcatccctt nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ccccggagca cccgcagggc caccctcccc
     1441 atgtcctctc cccccgccgc gtaatcccgg gccaccgcca ccgccgcccc ccccgagagg
     1501 gcgttggcgg catccatgcc aaaaaggggc acccgggcct cgaggcacgc cttagatatg
     1561 ggggcaaagg acgcgaaaaa gagatttccc gtcacctgga gcactgcgtc caccctttgg
     1621 gccaaaagcc cctggatgcc gtcggagacc tctgtgggag tggaaacggc tacggagaca
     1681 caggtgatcc cctgggccgc gagggccttc tcgacctgtt ttaagttata gacgcaattg
     1741 acctccgagg ggttgaccag cgtccccacg gtcttcgccc cgggaaggca ttccttcaag
     1801 gcccgcaccc cctcgacgta gggcgcttcg gtggagatcc ccgtgatatg agggcgatgt
     1861 accacatcgc tgctccccgc ccccgccagc actggattgg ccaccaggcc gaagaccacg
     1921 gggatatcgt ttatcttaga cagtacggcc tggagcgtgg gggtggaggt gagaagcaaa
     1981 aggtccaccc ggcggctccg cacgtggtcc accagggccg acagggtggg catgtccccc
     2041 tgggcgtttc ccacctccag gttgaagtcg cgcccctccc gaagccccgc ctcgtccagg
     2101 gcccgacgga acccccgcag ggtctcctcc atggggacgg actccacgta gttcaggaaa
     2161 taaagctcaa agcaccgccc cgcagggatc gccggaactc cctcggatcg gtccaccgag
     2221 acctccagcg ccccgctttc gccgtagacc aacgacgccc cctggaccac ctcggggggg
     2281 agctcccagg ggtcccgaag ccgggacgcc acctggcggt tcagggacag cttcaggggc
     2341 atgacgttct ccacggggat gtccccgggg tagagcccct tcatgatgtc tcccgccagg
     2401 cgcccctgga cgcgccccac atccacgtag ttggccccga gccccgcaag ggctccccgt
     2461 cccgcataca tggaggcata ccccagcacg gggatcctcg ccccatccgc cgccttgacc
     2521 acggagtcaa aggcggactc caccgtgtta tcccctccga tgaccagggc ctggacccct
     2581 cgggaaacca gggccgacgc ggcctcgtac accgccgacg agttgtccac ggtgaccaca
     2641 tcgagctcta tccccaggga ttcgcagacc tctcgagcca cgccgtaaca cgattgagag
     2701 caggcctccg aagggttcat caccgtgccg atgcgtgtta gccccgggta gagccttcgg
     2761 ctcagctcca gcgtctcccg tacgggctga aaggtgccca cccccgtgag ccaggccgga
     2821 tgacttcggt ggtcctcccg gctgatgccc acctcggcgg cgtaggggtc ggtgacggct
     2881 ccaaagatat gcacccttcg gccctgctta ttcgccgagg ccgccacctg tagcatggga
     2941 gtgctcacgg tgagcaccag gtcgtaatcc cctccaaagg ccctctgggc catgctcacc
     3001 gccgtgggca tgtccccctc ggcgcaaaag acctggagtt ccatgccctg cccctccaca
     3061 aagccccgct ccgccagccc ctccagaagc ccccgaaggg tctcatccag ggtcacgctg
     3121 gagacccatt tgagaagggc cactcgtacc ttctctccgg ctcgcacctt tcggtggagg
     3181 gcttccgccg ggccatgctc cgggccccgt cgcccctccc ggcccccttg gtccgagaac
     3241 aaaagcactc ctccggcggc cacgatgaga gccaagccta gggagagatg tttcaaagct
     3301 ttccacacgg agattcctcc tcctttgtcc ctcgtctgcc cctcgtcccc ttcggagcgc
     3361 cccccggggg ataaagcctt cggacgggct cccttcgggg ctccctctca tcgggaagtt
     3421 ttcctggtat actacttttg aggtcggaag aaaacccccc agggcatgtt accaccaccg
     3481 gggtcgtttt tatggtagga tggggaaagg gaaagggaaa cccccaaagg gatcggactc
     3541 ggggtagaga acaggcggtg gacgcatggc acaggaatgg ctttctctgg cagagatttc
     3601 ccggcgcaca ggcatcgcgg aaagcagcct ccggcgatat gcgcgaaact tcgcgccaca
     3661 ccttctccag gagcaatccg ggaaacggtg ggtgtttgcc gaagagtccg tggaggtttt
     3721 tgatcgcatc gggaccgcct accgagaggg aaagaccacg gaagaagtgg cgcggatgct
     3781 tgagaccact cccacggtga aaagcatcga ccatacatcc cccggagggg gcccctccgt
     3841 ccctgctccc gcccatcagg gcgcggcccc ggagatcctc cccctctctt ctgcgtcggt
     3901 ggccctattt ttctccacgg tccagacggc catggagcgc atggcggagg cgctggaagc
     3961 cgttcaacac cgggaccagg agcgcgtccg cctcctggag gaaaaaagca cctctcagga
     4021 aaatctcctg gaagaccagc gcctgcgcat caaggagctg gagcagaagc tccatcgggt
     4081 agaatcgctg cttttccttg cgggagatga aaagaccacg cccttggtgg taaaggatca
     4141 gctggaaaaa ctccagggag acctggccat ggtgcgccag atccaggaaa aagaggcggt
     4201 ggtgcgcatc ctcgaccagc ccacgccccg gtcgccgtgg tggcggcgcc ttttgggcct
     4261 taaaccttag gaccgggggg gctctagagc ccccccggtc ctaagtctaa agcagccctt
     4321 cggggactcg agataccccc aggcccaatc ccctaggacg cccctgattt tgcccatccc
     4381 catcagtcca ggggaagaaa ctcccgcacc ttcgccagga tctcttccgg atcgaagggc
     4441 ttggcgatgt gtccctcccc ccccgcccgg ctcccccgct cccggtcaga ggggtggatc
     4501 cgggaagtca ggaagacgat gtgcacgtca tcgaagcccc agaccttctt gacggtctcg
     4561 cagacatcgt atccgctctt gcccgccatc atgatgtcca aaaaaaccag ggtgggacgc
     4621 tctttcttga tgacggagag ggcctcatgc ccgtcgtcgg cgaaaaggag ctctacgccc
     4681 ttgtcttcca tggggcggag cagattttga agcagaagcc gaaccaccgg agcgtcgtcc
     4741 accaccagta ttttggccaa cggcccttcc ccctcacctt taggaagatg acaacccctt
     4801 ctcaatctcc cggggcacca cattgccgat cagtataaca aaaacccacc cctaaggggt
     4861 accttatcga atttttttgg aagggaaatc tttctcttct gcgaagcctc ctttgctcga
     4921 agatctcgga ccgaagagag atctggcgga ccccttcggg ccttgacgcc tttcccggat
     4981 atggataaag tggcgatgac atcacacccc attccataaa cccagggggg aatcttcgtg
     5041 gaaggcgaca gtttgcccgt aagatccaag acccttttgc cagatcgcta atcccgccgg
     5101 atcggcccgt ccggcgatgc cgggcgtggc ttccataccc ccactcccga acccgtacac
     5161 aagacaccgc agcccttgcg ggggacgatc ttccccgccc gagaagggcg tggcgtgccc
     5221 ccggggccct ggggcaacaa aggggaggga aacccatgat aaaagtgatg aaaagcgtcg
     5281 ctggcaacgt gatccgggag ctcacgctga acaccctgga ggagggggct tggatccacc
     5341 ttttggcccc cacccgggac gagctgcgat ccgtggcgga ggctaccgcc attccctttg
     5401 accttttggc cgcccccctg gacgaagaag agacctcccg ggtggaggtg gatgacgagg
     5461 cggtcctgac tttgataaac ctgccgaagg tgagcaaaga gggcggctac gacaccatcc
     5521 ccgcaggcat cgtcgtcagc gagcgccaca tcatcaccat atgcctggaa gaaaacgacg
     5581 ccatccccca ggatgtcgcc agcgccgcct cccgccactt ctgcaccttc aaacgcacca
     5641 gatttctctt tcagatccta taccgcaccg cctcccttta tctgcgatac atcggccaga
     5701 tcaaccgccg ttccgacgac atcgaggacc gtctgagggc ctctatgaaa aatcaggaac
     5761 tcttccagat gctggacctg caaaagggac tcacgttttt caccgcttcc atccgatcca
     5821 accgggccgt ggtccaaaag ctcctgcggc tctgcgccaa ccgccaggtg agcaagttca
     5881 taaaaacgcg agaagaagac gaagacctgc tggaagacgt gagcgtggaa ttcgatcagg
     5941 ccttcgagat ggttcagatc tacagcaaca tcatgaccaa catgatggac gccttcgcct
     6001 ccatcatctc caacaatctc aacatcgtca tgaaggtcct gacgctgatc accatcgtca
     6061 tggccattcc gtcgctggtg tccggcctgt ggggcatgaa cgtccccgtg cctttctctg
     6121 cggaccccta tggcttttac atcgtggtgg cctttgtcct ggtgctcgtc gtggcatctt
     6181 tcgtggcctt ctggaagaaa cgcctgttct agggggcgat cccgaaactc cttttccccc
     6241 ttgccccggg acgccccttg atttttctcc aggaactctg gcataatact cccgcgacac
     6301 ccctgcgctt ccatgcctaa gagaaaggag ggagccccgt tgaccatcca attatggatc
     6361 atctccgacg acgaagcgga agagcgccgc atcgaccata tggaggagct cgtgcgggaa
     6421 attttggatg agagcggcat gatctacgcc cggggagagg cgcgtcacaa agacgattcg
     6481 ggaaagcaca tttacctcac cgacattcag tgaccttccg cgcccttcgc ccccggggaa
     6541 tcgtcgtccc gccttcccgg cgggttagcc cgtccctggg cggtggagga atccatttct
     6601 agattggctc cttgtacgcc tcctcgagat ttttgtataa tcgaagcgcc ccgacgatgg
     6661 cctttggatc aatcctttcg ggagcccagg gaggcccttc atgttcgcac acgcgttcca
     6721 aaatctcttc cgggccctga agtcccggaa ctacaagctc tttttcggcg ggcaagccgt
     6781 gtcgctcacg gggtactgga tgcatcgcgt ggccatgagt tggctggtgt atcgcatcac
     6841 aggatcccct ttggccctgg gaatcgtgga cttcatggga cagatcccca cgctctttct
     6901 ggggtttttc gccgggacct tgttggaccg ctgggacctg cgccgggtga tcatcgtctg
     6961 ccagagcatt tgcatggtcc acgccctcct tctggcgacc ctcaccctca ccggggtggt
     7021 gcaatattgg cacgtggtgg ccctcagcgc cgtgttgggg gtggtgaacg cctttgagct
     7081 tccggcccgc ctctccttcg tggtacacct ggtggacgac ccggaggacc tgagcaacgc
     7141 catcgccctg aactccaccc tcttcaacgg cgctcggctg gtgggaccct ccctggcagg
     7201 ggtcgtcatc gccatgctgg gagaaggact ctgcttcctg accgacggcc tgtcctacct
     7261 cgccacgctg gtggccctcc tttccatgcg catgacccgc tcggccaggg cggaagccca
     7321 acctggaaag atgcttgaag agacccggga agggtggcgc tacgccatgg gctttctccc
     7381 catccgcacc atcctgctcg ccgtggcgtc catcagcttc ttcggcctgc cctacctggt
     7441 cttgctcccc gtcttcgccg gggacatcct aaagggagga ccccagacct tgggatttct
     7501 caccgccgca tcggggatcg gagccctttt cggctcttgt cgtatggccc tgcgctcctc
     7561 tcctgtgggg ctctcgtccg tcatcgcccg caccatgggg ctcttcggcg ccgctgtggc
     7621 gactttcgcc ttctcccgcg tcgtgcccct ttccatggtg ctcatggccc ccataggctt
     7681 ttccatggtg gtgaccttca tcgcctgcaa caccctgctc cagaccttgg tggacgacga
     7741 caagcgaagc cgggtcatga gcctatacaa catggccgtc atcggcatcg cccccctggg
     7801 gagcctctgc gcggggtgga gcgcctccgt cgtgggggcc cccctcaccc aggcgatggg
     7861 aggcgtcgcc tccatggtca cggcgttcct cctctggcgt tccttgccct cgatccagca
     7921 atcagcccgg tccaccatgg tggccaaggg ctttctttcc tccgaaacgg cgtgacgaag
     7981 gggcgacggc aaaaaaatag agaactccct agcgggggtt ctctattttt gtctggtatg
     8041 agcttttggt ccccggggtc accccaaccg ggagaagacc tcttcctgga gaatcccccg
     8101 gagctcttcc agcatggcgt tccgacgttc cggctccacg cagcccacgg caaagaagaa
     8161 gtggtgcaag atcacgtcca tgccgcagaa gccgaagatt ccccgatcca ccattccccg
     8221 gaaggcttcg tcgaatcccg actgcaggca cgattcctcg cttccccccg tggtgttcaa
     8281 caccaccact tttttcctct tcaggagccc cacagggccc tccgggccat agccgtaggc
     8341 gaagccctgg gagaacaccc gatccacgta ccccttcagg atggcgggca tgccccccca
     8401 ccaaatcggg tggatcacca ccaaaaggtc ggcggccgcc agctgctcct gctcctggcg
     8461 cacatcctcc aggggctccc caatgcacgc cgccgccagg tccccccctc cgagcacggg
     8521 attgaaggcc atctcgtaaa gatcccgcac ggtgccctcg atcccctgcc ggtccagctc
     8581 ctccaatacc aagttcaata cggcatggtt gaaactctga gaattcgggt gcgcataaag
     8641 gatcatcgct ttcatggtcc gtccctcctc gaacacggtc gcaaaccgaa cttgccacgg
     8701 gagttcagta tatcatacgg gaaggcgttt ttatcagggg cggctttttg taggaaactc
     8761 ggagcttcgg aggtctaaag aaaacggacg aaaaaggtgg gctaaagccc cccgccccag
     8821 ggttctttgg acaaaaaggg ctcccacgtc ccatgctcga agacttccag ctcccccgtc
     8881 tccacggaaa aataaagcaa cagtatgcga agcccccggg tcacttgggc gtactggatc
     8941 gacggcatcc gccgcagggc ctcgaaccgg agcacccccc gatcgcgaag gggaccctcg
     9001 tcaggggaaa gggcggccgg ggagacgtcg aggatcacca cgttctccat agaaggcttt
     9061 tccaggatcc gggccaaaaa ggccccaaag gcctcctgag ccccggagat ctccgggggc
     9121 caggaggcca tcaagagatc cccgggccct gcgtcgaaaa gatctacggc gctgaaccgg
     9181 ggatcgggat ccactatcac cagggtgtgc ggcgcaaaat ccctccggtc ggaggacaga
     9241 aacttcccat agcccttctg taatctcttc atgacacctc ccaaaagaat agcgcctgaa
     9301 gcgccggatg ggctccttac gccagtatac cccacccgga ggggaaagcc cccgggagaa
     9361 aaacctccgc tgggaaggtc gagtggtaca atggaggcga agacactcga agatgaggag
     9421 gcggtaccat gagacaatgg aaaagaggat ctcttgtggc cttggcgctc tgcgggctga
     9481 tgtcgggagt cgccacagcg gggccggttt tcgacgccat aacccccttc cgagatgcgg
     9541 aacgccgggg ggtggatccc gctctgctct cttgggggca ccaacttctg gagatcgaat
     9601 ttccctacac atggagcgaa gccctggaaa ttctggagaa agcctatcct tacgcgggac
     9661 ggagtaggct ggagacctgg ctgaaatatg gcgccgagtt tattatgata gacggagagc
     9721 cccggtactt ctccgccgtg gtcaatagct ttgcccaccg ccacgacgac atcatgcgtc
     9781 ggaagaacca agaagagggc cgggctgcgg aaaaatttgc cgcccccctg cgaagcatgg
     9841 ccttcagcgg ccccgaagaa agctatcccg cctcgacgtt ccagcccttc atcaatccca
     9901 taacctatct gggcacccaa accctccgga tcccccgctc cgccctctcc gccgacggca
     9961 cgctgaaact ctggtttccc ctgcccatcg ccaccggcga ccaggatgca gtgcgggtcc
    10021 tttccgtcac gccggagcga tgggtccggg gactccccac cgtgaacgaa gagctggggc
    10081 agctctacat ggaagtgccc ctccaggagc tcagcggcga cctggacgtc cggctcacgt
    10141 tcaccttccg ccactgggaa cggcgctctg tggtgaatcc cgcccaggta gggacctacg
    10201 acgtcgccag cgagctctac cgtcgctaca ccgccccggg gatccacacc cccctgaccc
    10261 gggaaatcgt ggacaaggcg cgggagattg tgggagacga gaaaaaccct tgggtcgccg
    10321 cccggaagat ctacgactac atgctgaaac acgtgcgtta cagctttacc ccccaccttt
    10381 ctctgggatt caccggcact cccgaagctc tttacgtcct ccgcaacggc ttcggcgact
    10441 gcggcgccca gagcatgctc ttttcggccc tttgccgggc ggtgggtatc cccgcccgca
    10501 ccaccggagg gtaccagatg gtgccccggc acggcggcac ccacttctgg gcggagttct
    10561 accttcccgc ctacggatgg atccccgtgg acgtgaccat ggcggacacc gcctcctggg
    10621 ccgacatccc ggacgaagag cgcatctcct tcgagaacta ctgtttcggc aacatggatc
    10681 ccttccgcat ggtcatccag acggacgtgg agatccccct ccaaccccgt ccggagagcc
    10741 ccgtactcct catggctcct tccggggtcc ccatggtgtt ccagaccccc gccctggtgg
    10801 cgccccattt cgccgtcacg cccatggann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10921 nnnnnnnnta gtgcacgttg gggtcctcgg gatcctggtc cacggggtct gcgtagccgt
    10981 ccccatcgga atcgggatgg acctcgctgt tgtagttggg aatggtgaat cttgccgaga
    11041 ggaacatggg ggcgaagata tccctccacg ccccgatcca gagatcttcc ggacagctct
    11101 ggacggcgca ctggtacgac gtgctcaagg gcccccgggc caggatggtg atctggatgg
    11161 accccagccg gagctccccc gaaggggctt gccaacgcac gatgaagacc gtagacaccc
    11221 ccgtatcgtc ccccgggacc gagatccccg ggagcccctt gcgctccatg gtcaccagcc
    11281 gcaccggatc ctgccccacc agtcgataga acaccagatc ccgcaactcc tccgccgagg
    11341 gcatccccgg gacctgggcc acctgggcga agataaagtt cgccgccccc gaggggtcgc
    11401 tcacccaaag gaattggggg ttcgcgtccc gaaggcccca accggggggg tactggaagg
    11461 accagtcccc tccggcatag aggggccacc cggggatgtc ccgctggggc ttgatcagcg
    11521 gcgcctggcg ccactcctcc atgaggcgct gaatctgggt cgccagggcg gggtccagag
    11581 gatcatccac cccccgggcc gccccttccg cccctcccag ccccaccgcc atggccaaaa
    11641 ttaccccaaa aatccttcga agcatcctca tcacactccc tcccgtcggt caccctccat
    11701 ctcctgtgcg aaagaccttc gtcccacgcc tcggcccaaa gcccccggcc aaaccctagg
    11761 accctcaccg ccgatggagg cacaccagca cttccacgtg gggggtcccg gggaaaaagt
    11821 cgtacggatg gagcgacacc accgcgaagc cctcccgttc cagcaccttc aggtctcgcg
    11881 ccagcgtccc cgcgctgcag gaaaggtagg ccccccgggc aaagctcccc cgggatccca
    11941 tccaggcccc cacctccggc tctagtccca gacgaggggg gttgacgtaa aagagccgcc
    12001 gctccggagc aaaggcctcc aaccaccgct ccacctgggg caagcgctgg gcgcagcttc
    12061 cccgaagcaa cgtcgccccc gggacgttga ccgccgcgca ctccagggac tcacccccca
    12121 tctccacccc cagggccatc gccccccgct cctgccaacg tcgcagactc ccccccgtac
    12181 cgcaatacag atccaccacc ccatcccctg gcttcgggga caaaaaggct tctgccctct
    12241 ccagggcctc gaggtgaagg gaggggcgta cctgctgaaa cgacctaggg ccgtacacca
    12301 ggccctgggc atcgcggctc cggggcactc cccaaaggag gcgccacgaa ccacgcccga
    12361 agacccggcg tcccgcgccg gggtgacggt gcaaccacag cccctccagt cccgcagctt
    12421 ccccgggatc cggcgccgtc tccatccagg acagatcggg gtcctcccgg gatttgagca
    12481 ccagcgtacc ctgccccccc gcctgcacat agtacaccaa gggaaagttt tccgcggcgg
    12541 gaaggtggtg ccgcagccag gaaatcatgt tccgcactcg ccgggagtgg cgcgggcatt
    12601 ccggaatggc caccagcccg tcctttcggc gcaggcccaa cgcccacccc gtttccggca
    12661 gccaggccgc ccggaggcag accttgtccc gataccccca gcgctgctcc tccggcccat
    12721 cccttaaatc ctcccgccgg tccttccaga gggccaggga tttttccagc caggccatct
    12781 tacggtctct ctcttcggcg gccgaggcca tgtcgggggc gcaccctgga cagcaaggca
    12841 cgcaaccccg gggcaaacct ccgcccttcc actcttccca ttccccgacc acgacgttta
    12901 cccctcccta cccaatggtt tcccatcgag gcacaaattc cgatggccca tggggccttt
    12961 atggaaagaa ggacgaagcc cataaccacg ctgacgacct tctcttaccc ctctcacgat
    13021 aaaacatcgg gaaagcgcag gagttgttgt ttatcaagaa agatatcaaa ggctcttgac
    13081 ttttcccaca aagggtatta gagtgcgtct tatatcaata gcaagggagg aaatgacatg
    13141 aaagaccagg cagatattat gaaccaactc aaggcgttgg aaggaaagac cggtcgaggg
    13201 attatatccc agttggtaac cgaagaaacc ctgggctata tggagaagct gaaccgagat
    13261 ggggtacctt acgccgctgt ggcggagaaa ctttccgcat cgacgggaga aacggtcaag
    13321 cccatgcagg tcatgttcgc catccagcag taccgcaagc gccacaacat ggaacccgtg
    13381 cgatcttcct cgggaaagcc ccggggacga aaaccccaga cacctcnnnn nnnnnnnnnn
    13441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13501 nnnnnnnnnn nnnnnnnnnn nnnnnnccgt cgggcttcgt ccccacgacg cagtccgacg
    13561 gaggaagccc ccctcttctt gaaaaagccc gcgcttcccg ataaatttcg tgaattttac
    13621 caaattgctc cggcacttcg gcctcgcgcc gcctcttcca gcacgcgctc aagcaccgcc
    13681 atgttcgtct cggaggaaag ctcccgtgcc gcccggtggg agttttcctt gtagaccctg
    13741 acctgttccg gagtcagctc ccggagcctg gccgccagcg cttcggggga gaaatcttct
    13801 cccaccacgc cgagctggtg ctggcgcacc aggcgcccca tctcgggaga agggccgatg
    13861 gccaccatga gacgagcctg cacgaagtcg aaaaacttat ttggcagggc gtgggcgtgg
    13921 ttgaagttcg atggcggcag gatgtacatc cccccgtcgt actcccggag cgtggaaacg
    13981 acgtcccgca gaggtacggg ggggcggatc cgcactcgcg ggagggcctc cgcccgggaa
    14041 accatggcgt caaagtacct ccggggaggc cccggcacga gaaaaagatc cagagaaaag
    14101 cggtcgtcca gcaaaaccat ggtgtcaagc atccgatcaa tgtgtcggga aggggcgcag
    14161 atgccgtggt gaaccaggcg aatttttccg gggaccatct ccgacggcga gagttccaca
    14221 taatccgccg cattggtcac cacctggggt tggggcaccc cgaaatttct ggcataggct
    14281 tcggcgatgc cttcgctcac cgtgaccatg ccccccaggc ggggcaaata agtgcggcaa
    14341 agccattcct tgtacgggcg aaaaaacagg cgccagatcc acacgtcttc gtactgccgg
    14401 ggagagtatt cgtgggcgtc aaagaccacg ggggcgtctc cacagaccga aaatccatag
    14461 ggaagggcgt tggcgtcgtt gcagagcacc acatcatatc cccccggcat caccccgggc
    14521 agacgcagat ccccatgggc aaaacagcgc tcgtaggcgt cgtaacgtcg cagagccagc
    14581 agaggaagct ctttcagctt ctcccggtag ctcggcacgc gcccttgggc cacgtgcacc
    14641 gtcacatcgg gtaggcccgg atcggtgtag gccacgcact ccacccgatg ccggcggcgt
    14701 agggcgttga gctggcgaaa cacccgggcg tcgctccgga ggtcggaaaa cgagatgacg
    14761 aggattctca acggttttcc cgggcgccca aaaaggcggc aagggcccgc accacccgat
    14821 cccccgcagt gccgtcgcca tagagcccgg cgggatacgg caccgacgtg cccagggcgg
    14881 cgaaggtccg cgtcatttcc tgggcgtccg gagcacaaag ccggtgccac ccggcctcca
    14941 ccagctcccg ccaccccgtg tcgggcatca tcaccagggc ccgcttttcg gcaaagtatg
    15001 cctctttctg aaatcccccg ctgtccgtca ccaccgccgt gcaaccttcg agcaacccca
    15061 tgagttcaac atatcccagg ggctccacca gccgaagtcc cgcagtgagg tcttccagag
    15121 cgaagtcctt caggcgctgt cgcgttcggg gatggacggg gagcaccacg tcgaggccat
    15181 actccctccg gagctccgca aaccctttca gaagttccgt caggggctcc ctcgtatcca
    15241 cattgaaatc ccgatgcagt gtcgccagca cgtagggacg ttcctccacg ccaaattttt
    15301 tcttcatgtt ttccgggcgg aagaggagac gcagggtcag gaagaggtcg tacatcacat
    15361 cccccaccac ctccccccgg gctcccagcc cttcggcctc cagatttttc cgggccagtg
    15421 cggagcaaca gtagagacga tcggccagat ggtcggtgac cacccggttg atctcctcgg
    15481 gcatgtcccg gggagcctga cgaatgcccg cttccacgtg ggccacgggg atcttgagct
    15541 tcgccgccgc cagggccccc gccacggtgg tgttggtgtc gccatagacc agcaccaggt
    15601 ccgggcgttc cgccagaaga agttcttcca cccgcgccag ggcatcggcg gtctggcgtc
    15661 catggcttcc ggaaccgatg ccaagaaaat agtccggctg aggcatggcg agctgggtga
    15721 aaaagatatc ggacatgttc acgtcgtagt gctgtcccga atggaccacc acatgtcgcc
    15781 aggcccccag gcggcgcgcc gccgccccca ccaccgcttc tttgataaac tggggccggg
    15841 ccccgacaat ggacatcacc atattcccca tcgctgtgcc gtcccttccg ctcgcgagcc
    15901 gcaccttttt cggagaaaag ccttcaggcc agaaagggcc gaagctcttc gtccgaagga
    15961 aaattgatca acaccgcccg gcgtttgccg taaaacacga agagacttcc ggcggccacc
    16021 gccgaagcgc ccccctcgtg gaccgccgct ccaaaatcct ccacctttcc cgccccaccg
    16081 cacgccacca cgggaactcc cacagcggac gccacacttt ggatgaggga aatgtcgtat
    16141 ccctgctgcg tgccatcccg atccaccgag gtgagaaaga tctctcccgc gccgcactct
    16201 tccatctttt gagcctgtgt cacggggtcc acatcccgga ccttctgtcc tccatgggta
    16261 cacacgcggt acgagcccca gaaatttttt ttgacgtcca gagagatcac catggtggag
    16321 ctgccgtact cccgggcggc ttcccggacg aaggcgggat tttcgaaagc cgccgtgttc
    16381 agcaccacct tttccacgcc gaggcggagc aatgcggcga tgtctgccat ggtatgaacg
    16441 cctccgccgt acgaaaaagg catgaagcac tccccggcga tgtccttcac cagatcaaag
    16501 cggggccccc gcccttctct cgtggcggtg atgtccaaaa acaccagttc gtggcactct
    16561 ttttcgttaa aaatcctcac ggcgttcaac gggtcgccga tgtaggtgta gtcggaaaat
    16621 ttcacgctct tgtacagccc atctcctcgg aggagcagac aggggatcac ccgcgtccgg
    16681 atcatctccc caccccctcg cagaaattcc gaagcaggcg catgccaaac cgcaggctct
    16741 tctcggggtg aaactgcacg ccgaacacgt tgtcccgccg cacggcggag gtaaaggttt
    16801 ccccgtattc cgaaacgccc agcacgtcgc cgggatctgc gcaaaccacg tggtacgaat
    16861 ggacaaagta gaacagagcg ccgggctcca tcccggcgaa aagggcctcg ccgctccgcg
    16921 ggatcaccgc attccatccc atgtggggca ccttgtgctg ccggtcctcg gggggaaagg
    16981 aaaacttccg gcacacggcg tcgatccatc ccagccccgg caatgtgccc tcctcgctgg
    17041 accgggccag aagctgcatc cccagacaaa tacccagcac tggaactccc tcgtcccgaa
    17101 ctctgcgggt cagcgtctcc agaagccccg aggcctgaag attgcccatg ccccgatcaa
    17161 aggcccccac accgggcaaa agaatggcgt cggcccgctc cagcacgtcg ggggtagaag
    17221 ccagttcgga ctcctgtccg atcttgtgta gcatgttgcc cagagatccc acgtttcccg
    17281 tgccgtaatc gacgatggca atcaagatct tttccacgct cctccgccca ggcggccata
    17341 ggcttctacc atgctgcgca cttctttgtc ccaatccagc tcccgggccg cccgctccgc
    17401 tccggcccgc ttttcggcgt aatcgttccc cgccggatcc agttggttca ccaggtgcac
    17461 caaatcttcg gggttcccgt tccagatcca tccacaggaa aacgcttcca caatccgccg
    17521 ttgctccggg aggtcggcga tgatcagggg tatgggggcg aaaaggtact cgaagatctt
    17581 gttcggcagg gaatagaggt tgttcagggt gtttttctcg aacaagcaca gtcccacgtc
    17641 cgccccctgc gccaccgaaa gcacctcttc ggggggcacc gggggcaggt aatggacgtt
    17701 tgagacctcg ttcgcccggc gacagaccac gtccttcaga gggccgtccc ccacgaagac
    17761 cgcgtgccgc cccccccgga cgccctcgaa agccttcagg atctcctcaa cgccccgatg
    17821 gggccccatg ccccccagaa acaaaaagag cgtggcttcc ggcggaagag aaaggcgctg
    17881 acggaggttc cacgcctctt tccgcaccag ggagaccccc cggggaatgt tacgcaccac
    17941 catcggccgg gaaactccat acgcccgaac gtaccaatcc gcgatggcgt ctcccaccac
    18001 gaaaaacgcg tcgacgcgac caatgagcca tcgctcaagg cgcttcgccc atgccttttt
    18061 cccgccggaa aaggcggcca tctcggtctc cagctcgtgg gcgtcgtaga gcaccggaac
    18121 gccccagagc atcttcgccg ccacgcccac gggcagcgcg ggaagactgt ggcagtggac
    18181 cacccggggc ctgtttcggc gcaccgcgtg aagtgcccgg acaacccatt cgacgtactt
    18241 gcagaggtgt cccaccaccc cccaccggag cttccggctc cagagccgca tgcgatccac
    18301 ccgcactcca ggggcgaagg actcccccag ggggagtcct tcgtcgtgta accccacaaa
    18361 caccacctcg tcaaagagcc cctcttccag caccacagcg gcctctttgg cgaagcggga
    18421 ctcccgggtt ccattgctca ggaacacgtg aaccacgcgg ccctgggtca acgccgcacc
    18481 aatcccttga gggtgcgcaa cgtctgcacc atgccaaaat gagagtcgta atcgcgaaac
    18541 gttttgtggg gcatggtgag gaactgctcc agatcctcgt cgcggaggcg aagtttcttg
    18601 acgatgtact cccgatccgc ccccaactct ccctcctgat acaccggccg ggccatctcc
    18661 gccagagctt gatctctcgt catctggccc gaaacaacca gactcgccag atgggcccgg
    18721 cgcttttcat agccaaatct tctgggaagg aaataatact ggaaaaattt cgtaaaccgc
    18781 gactcgcagt gtttgcctcc gtaatgcttc cagccaaact cccgctgcaa ctcccgcacc
    18841 gcttcttccc gcacgtaggg aaggaagttg agcggtttga gcaccgtcat ctttttcaca
    18901 taggggtagt gaaaatagta ctgataaaag ctgacggtgg ggtagtttcg gaggggaatt
    18961 tccccgaacc tccgatgaat ggaccgaagt tgtctggcgt ccatggcgtc atacccccag
    19021 gacgagggga gaatgccttc cgtggcgtag ttggagccgc tcagcacgta ccgaatgccg
    19081 tttcgcaccg caaaggcgta cagggccgca aaaaaagcgt ggtcctgggg cacatcctga
    19141 ttcgccacac cggagcgcaa gaaggccact tggagatccc gcatctcttc ccaatccacc
    19201 acaaaagtct ggagatcgag cttcagcttc cggcacaggc tctccacgtt tctgaccgcc
    19261 acctcggagt tccatccccc gtccacgtgc atcaccaaag gccggagccc catttgcacc
    19321 acctggtacg ccatgtagga gctgtcgacg cccccgctca ggccgatgat acagtcgtat
    19381 tccttgccct gcccctcgcg gcgcacggtt tccagaattt tttccaggcg ttgccgcccc
    19441 tcggcgttgg gccgccagag gggccggagc tcctcatcaa aacgtcgaca gtggttgcag
    19501 actccccggt cgtcaaaggt gatctccgga tccgtggtgt ccatgataca tcgtgtgcac
    19561 tgtctatagg tcatgatgtt ccctcccgtt cttctttcgg cttttccaaa cggcgcagaa
    19621 tgcgggcggg gtttccggca atgacgcagt gcccctcggg aaagctcttg gtcaccacgc
    19681 tccccgcccc caccaccgtc ctctcgccca actccactcc aggaagaatc accgcattca
    19741 tgccaatcca acagtgatct cccaaggtga tgggggatcc ttccacatga cggtccaaat
    19801 tccaggggtc atggttggcg ttgatcaacc ccacattggg ggcaatgtac acgccatagc
    19861 cgatggaaag aggccccccg cactggaagt agcaccccgg gctctggaag ttgtccaggt
    19921 tgtccggatg gaaccggacc attcgggggt cacccacggt acagaggggg gacacgggcc
    19981 aggggatacg gcgattaaaa ccaaagattt tctgccacca gacgccgcgc caggcccaga
    20041 agatcccatc cagcacgggc caacaaaacc aacggcccga aagataggat cgttgaaaaa
    20101 aaaagggaag cacgatcatg gaaatcttga aaactaaagt ttttattatt tttttcattt
    20161 ttattgtttc aactctttca attttctttt gagtaaaaaa acccacatcc ccgcagacat
    20221 caacgggaga gtaattatgc atatcgtgat ttccgagaaa taccacaatg gtgccaaggg
    20281 aaccaaacag gtgacaagaa ataaaaaaga ctcttttata gaatttagaa tatcagaaaa
    20341 agaaacgtga gaaaatttga gcaccaaaaa caaatatgaa atatagaaga atacattcgc
    20401 tccagaaaaa agaacgatag ccccttgcat atcgaaatac atcaatccta gggaaatgga
    20461 ggtaattcga agacaaagct gtacaagaga acaaataaaa tcacaccgct gctgaccccg
    20521 gatatacatg acagacgaga ggggggacgt caggaactgg gcaaaaaacc agggtcccat
    20581 gacggggagc aaagctcccg cttccttcca tgcggcgccg aagatcaccg gaaaaagttg
    20641 cggtcccagc aacaccgtga cggcaacggg atagagtccc acaagcatga ggcctcggaa
    20701 aaccttggcc acccacacac ccagatgccc ttcccgggag agctcacttc cccgctgaaa
    20761 gaaaacctgg gccaccgcgt tccccacaaa catcatgggc acctgcagga ccttcatgtt
    20821 gaagaacaag atccccgccg ccgccacacc gccaaaccgg gcaaacaaga tgggaacgat
    20881 ctgggagctt aaaatggccg acaggttggt ccagggttgg aagaggggaa atctccggaa
    20941 cgccaccaca tcgtcccaga gctcccgaga aaaaagctcc cggacactgc cgggcggcac
    21001 caaacggcgg cgcagcacgc tgatccccgc cgccatattg cacacctggg aaaccaccaa
    21061 ccccagaggg cgcactccca aaacgcccaa ccccaccccc gacagagccc cccaaacacc
    21121 ttgggagatc cgggtcctgg caatggtggc aaaggccttt tcccgaatgc ccaaatagct
    21181 gaaaatatcg tagatgccgg aaagcaggaa ccccaccaga aggcatccca tcagaagcag
    21241 atcggaaaaa aaggggtact cccggcgcag cgctccctgg aaaaagaaca acaggacaga
    21301 aaaaaccacc gccataaagc cgtgcgcaag aaaagccgct cccgcgaccc tccaggcccg
    21361 agaacgggtt tcggtcagtg ggatggccag atgcagtccc agaccggaaa aggcggtcat
    21421 ggcgctgaga aatgccgcat agaccgccag aacgccgaaa tcgccgggag catagagccg
    21481 ggaaagcacg ggggcgcaca caagggccac cgcctggccg aggaaggctc ctccgcccac
    21541 gaaaacgaca tcacggaaaa agggccagcg ctgaagtttg ccctccaggc tggctcgaag
    21601 attcttcagc acttccgcag gccgtacgcc gcctcgataa tcgccacggt cttgcggcct
    21661 tcgcggccgt ccgacagggc ctcttccttg ccgtcaagca cgttcaggac gtgacggtag
    21721 tagggaaggt ggccgaaacc gtagacgctc gtggggttgg tattcgcttg ggcgacttct
    21781 tcgtccatgg ggtgggagtc ggcgaactcc cagtgttcca ttttgttcag cgccacgccg
    21841 ccgatgcgca ccgtgccccg ctctcccagg agcgtcaggc tcccttccag gtttttcgga
    21901 taggccaacg tggtgacgtt gatgctcccc agggcgccgc tacgaaagcg caggttcacc
    21961 accgtggtgt cctccgcttc gatgcgccgc tccagcgtgg ctccgaaggc cgagacctcc
    22021 gccacggagc ccccgaacca ctgcaccagg tccacgtagt gggcggcctg attcgacagg
    22081 caccccccgt cgagctccca ggtgccccgc caccgggcct ggtcgtagta atcctggggg
    22141 cgctgccaga agacgttggc catgaccatg tggattcggc cgaagcgccc cgcctccaga
    22201 gcccgccggg cgagctgcac ggtctggttg aaccggttct gcttcaccac gaagagctgc
    22261 acccccgcgt cgtcgcaagc ctggatggct tcgtcacagt cttccagtcg gcaccccagg
    22321 ggcttttccg acaaaacgtg gcgccccgcc cgggcggcct gggccgcgtg ccggggatgg
    22381 agccccgagg gggtgcagag ggtcaccagg tcgcactcca cctcttgaag catcttcgcg
    22441 taattgtcat aggcctggca gccctcgccc accttgcggg cggcctcttc cgcccgggac
    22501 cggacgatgt ccccacaggc cacgatgcgg gcgttctcca tctgggccac cgcttcgaga
    22561 tggtttttgc tgatccggcc gcaacccagc agcgcgatac ggtacacttt catccctccc
    22621 cacgccccta cagcagcgcc aattgggcga tgtccgccgg agactttttc agcgccggag
    22681 ccaccacgtt cttcgcgtcc agaatcagcc gggcgtgctc caccagaagg gcatagtcca
    22741 cccggtgccg gtgccccgaa gccaccacca ccgcatcggc tccccgcagt aggtccgccg
    22801 agagctccgc cgtggcccgg gtctccccat tccagcgcac cgaggggcaa tagggatcca
    22861 ccggcgtcac ccgggctccc ttgtgctcca ggagctccca gaccttgagc gcgggggatt
    22921 cccgcagatc gtcgatgtca cccttgtacg ccaatccgag gagcacgata tggctccctt
    22981 tgagggcctt gctctggtcg ttcagcaaat cctgaagccg ggaaaccacg tattccggca
    23041 tggcgtcgtt aatctctccg gcaagctcga tgagccgggt gtggtagtcc acctcccggg
    23101 ccttgtaggt gaggtagaag gggtcaatgg gaatgcagtg cccccccacg ccggggccgg
    23161 gatagaaggg catgaagccg aagggcttgg tggcggcggc ggcgatgact tcccagaggt
    23221 tgatgccgag cttgcggcac accaccgcca tctcgttggc cagggcgcag ttcacgatgc
    23281 ggaaggtgtt ttcgaggatc ttggccattt ccgcctcccg gggcgaggac accacgaaaa
    23341 cttccgcttc gagcaccgcc tcgtaaaggg cccgagccac ctcggcacag gcgggggtgc
    23401 accctcccac caccttcggc gtgttttttg ttttaaaccg aatatttccc ggatccaccc
    23461 gctcggggga gaacgccagg tggaagtctt ggcccacctt gagccccgat gcctggaaga
    23521 ggggaagaag gacttcctcg gtggtgccgg gataggtggt ggattccagc accacgagca
    23581 tgcccggatg gagccgggcc gccacttcct tgccggagtt cgtcacgtag gacagatcgg
    23641 gctgcttgta ccggtccagc ggcgtgggca cgcagatggc caccacatcg cactcccgca
    23701 gacggtcaaa atcggtgctg gcttcgatgt gccccgaggc caccaggcgg gtcagctctt
    23761 cgtccaccac atcgccgatg tagttttctc cccggttcac ccgggccacc ttgtccttct
    23821 ggatgtcgaa gcccagcacg cgaaaccccg ctttggcctt ttccaccgcc agggggagcc
    23881 ccacgtaccc caggcccaaa acccccaccc gagcctggcg ctccttaatc ttttctagca
    23941 acgtcatctg gtccgatccc ctctttttcg tcagccttcg tagtaggtct ggttttccag
    24001 acgctgttcc ggcgccacgt cgcgcaccac ccgggcgggg tttcccgcca ccactacgcc
    24061 ggggggcacg tcccgggtca ccacgctccc cgccgccacc agggcgtctt cgccgatctc
    24121 aagacctggc aacaacgtgg cgttggcgcc gatgcgagcc ccgcgacgca cccgggggcc
    24181 tccaaagtgc tttttgcgct cctcggtgcg cccgaggaaa ttatcgttgg tgaacgtcac
    24241 ttcgggggcg atgaagcagt agtcctccac cacgctcaac gccgtgacgt aggcgttgct
    24301 ctcgatcttc acccgccgcc ccacggtggt cttgttctcc accgtggccc cccgccccac
    24361 gatggaaagt tctccgatgg tgacgtcctc gcgcaccgtg gcgagatctc ccagaaaaac
    24421 ccggtctccc aagatggcac cccggtagag cacacacccg gcccccaccg tgacttcctt
    24481 tcccagcacc agcgggggca gctccaccgg ctcccccgtg gtggccgaga gcgacgccct
    24541 ggcggggagc ttgcccagaa tggccccatc gccgatgcga cacccctccc ccaaacgcac
    24601 gccctggcgg aggatgaccc cgtggccaat ctccgcatgc tcgccgatgt gcacgtcgtc
    24661 ttcgatgacc acgtgctgtc cgatccgggc ggtgcttgcc acctgcgctg ccctcatgaa
    24721 catgaactcc gcacctcctg cgtcagatcc acgaaagagg gatccccggc gtgaccgtcg
    24781 ctccagatca ccagggcccc ttccgcgaca aatttttcct gcaatcgttc gagctccccc
    24841 cgggagagca ccagggggcg cagcttcctt tccgtgaggg tctctaccct ggccaccagg
    24901 tcctggaggt acgtcgtctc cacgtcgccc accaggacta gatcgatgag tcccgaatcc
    24961 cgcccccggg cgtagtcccc cgtgacaaag gccagctcca cgttgcccag acgccgcacc
    25021 acgtgctcca ccacctggtc catgcccatg gtcttggcca cgatgctgcg tatttcggga
    25081 aaaagcggat gggcgctgtt ggccccgtag agcttccgcc gcccctcgtc ggccacgagc
    25141 aagagccccg cctccgccag gcggttcagt tccgtccgca ccgcattggt ggactccccg
    25201 aactcgtcgg cgagtccccg gagatgccct cggacatcgg ggttcaggaa gaattttaac
    25261 agaagcttga tgcgagtttt tgaggtgatc agcgtgtcta tcatgagtag aaatactact
    25321 caaaacgtcc ggtctgtcaa ggaaaaacgg gggtggagct ttccggcggt cttttgcagt
    25381 atgatggcgg aggtgaacgg ccgccctctc gagaagaagc gcttcgtcct ccggcgcctt
    25441 tgtgccggag aagtctccct ccgggaaaag cggtccccta tctcaggcac gaaggagtga
    25501 agccatatgt ccattgtggt ggcggaactt gtcgtttccc ccctcggcac ggggagtccg
    25561 agcatcagcg cctacgtggc cgacgtggaa cgccttctgg agaccttcgc cctcaaacgt
    25621 ctcctcaccc ccatgggaac cattctcgaa ggggagctgg acgaggtgct ccgggcggtg
    25681 cgggccgtcc acgaggcccc ctttgcccgg ggagcgctgc gggtttccac ctcgttgcgc
    25741 atcgacgaac gacgggacaa aacgctctcc atggagggaa aagtgcgcgc cgtggaagaa
    25801 aaactctgac cgctttttct tgaggccgcg tgcctggggg gctctgtcat ggcgatcgcg
    25861 cccggtccgg cggaagaagc cgtcccgggc gcgatgccgg agctttagca gttttccagc
    25921 atcttcacca agacgcccag cgaaaggact tcgttcaccc gctccacaaa ggggatgttt
    25981 tccggagcca cactcgccgt ggcgccccgc agaatcatgg gggccaggcc ccggtcccgg
    26041 gctccccgga aggtggaaag cacacagtat tccgcgcaaa aacccgtgag caccggcgca
    26101 tccaccccca ggcgtcggag ctcttcctcc agattcgtgc cgtgaaaggc gttccccgca
    26161 gtcttcacca cccgaaaatc gccggggcgc acgtcgaagg aatcggccat ctcgaatccc
    26221 tcggtgccgg ggatgacccc gtcctcgtcg tcctggtgct ggatccacac cacgggcaaa
    26281 ttctttttcc gaaacaaggg caccaccgca ttgatctggg ccaccgcctg ctccagtgac
    26341 ttccgggtct ccccgctgat gtgaaaaaac gccttctgta cgtcgatcac caacaacgcc
    26401 ggcttcattc tcttcacctc cgcatgtggt tcgcccctcg gggcctgtgc tttctggaaa
    26461 ttattttacc ggaaacaggc cttcgatgca aaaagccggt ttcgggggca cagaaaaaac
    26521 gtgagaaaaa acgccacttg ttgtataatg tgtgcgagta gcgcggttcc gggaaattct
    26581 gtcgtataca gcacatccgg cccagcgccg cgcgccaatc tcgttatgga ggtgttccgt
    26641 catgcaggaa cggcgttatg cccatcggat ggggaacatg cgggcttcag acatccgcga
    26701 gctgctgaag atgtccacca gccccggcgt gatttccctc gcgggggggc ttcccgcccc
    26761 ggagctcttt cccacccagg atatcgcgga cgccagctgt aaggtccttc acgagatggg
    26821 gcaggtggcc ttgcagtaca ccaccacaga gggctacgcc cccttgcggg agaagatcgc
    26881 cgcgcggatg aaccgccttt ttggcgcctc cgccacagcg gataacgtcc tcatcgtgag
    26941 cggttcccaa caggggctgg acttcgcggg caaactcctc atcgacgacg acgacgtgtt
    27001 agtctgcgag tgccccacct acgtgggggc catcagcgcc ttccgggcct atctgccccg
    27061 tttcgaagag gcccccaccg acgaccacgg ccttatcccc gaggagctgg caaagatcct
    27121 cgcccgggag ccccgggtca aggccatcta cgtcatcccc gatttccaga acccctcggg
    27181 gcgttgctgg agcctggagc gacgccaggc cttcatggag ctcgtctccc agtacaacgt
    27241 ggtggtactg gaagacaacc cctacggcga gctccgctac gagggggaaa atctcccctc
    27301 gctaaagtcc atggacaaaa aaggccaggt agtcatgttc ggcaccttct ccaagatcct
    27361 ggccccgggg atgcgcatcg cctggatcct cgccgagaag gatctcgtcg agaagttcgt
    27421 gctcatcaag cagggcgccg accttcagac ctccaccatc tcccagatgg agatcaacgc
    27481 ctacctggaa gataacgaca tcgacgttca cgtggaaaag atcaaaaagc tctatcgcgc
    27541 ccggcgggac tacatggtac aatccatcca gcggcacttc ccggcggagg tcaccatgac
    27601 ctatcccacg ggggggctct tcctctggtg caccctgccg gagagctgca acgcccggga
    27661 gctgctgaag atcgccctgg aaaagaaggt agccttcgtt cccggagggg gtttcttccc
    27721 caacggaggc cacgagaaca ccatgcgcct caacttctcc aacatgcccg aggatcgcat
    27781 cgaggagggc atttcccggc tgggagccgc cattaaggcg ttcctataga ccttcaccca
    27841 gagaaaccac cgctccatcc gccccatccg caagcgaagg gccccggcca cccgccggag
    27901 cccttcgctt tttctcccgg cgcggcccga ggcccccttc ccggggaggc gcccgccccc
    27961 gcccccacgc ttcctggcta cttcgccgcc ggggccacct tcaccgtcca ttcgggaccc
    28021 gaggatacgc cgtttttctc ggcgaagtta tccatgttga tcgccaggga gatgttcatg
    28081 aggctattga agtagagcaa gggcttccct tccggcacgc tgccaaaggt ctccgcgtag
    28141 ggaatgcggc cggagaagac ttccttgcct tccttggaga tgaccacgtc gaactcgtcg
    28201 ccgaacttgg actggaaggc gtcgaagagt tccttgccga tgttggtcca cacgttgccg
    28261 tactccacgt ccaacacggg gatggtgcca aagagggccc cctcccggga ttcggccttc
    28321 tgataggcga ttttcaccag ctgggcggga tccagtgcgg gccccacgtc ctccacgcgc
    28381 aacttccccc ccgccagctg ggccgccacg aaggagtaca ggtcccggcc gaagaaggtg
    28441 taggactggt cggacccggg gaggcggtgc ttttgttcgt cgatctgccg cacctcggcc
    28501 acccccagag actccgccac cagcgtgacg gtgccgttat ccggggtcac gatgatgtgg
    28561 ccgcttttgg tacgaaggac cagggagcgg cgctcggtgc ccaccccggg atctaccacg
    28621 gagacgaaga cggtgccctc gggccaatag gccacggtct ggtagagccg gtacgccccc
    28681 tcccagatgt tgtagggggg gatctcatgg gtcagatcgt agagtttcag ctccgaatcc
    28741 acccccaggg ccacgccctt catggccgat accgcgccgt ctttgagtcc gaaatccgtc
    28801 tggtagacca ccacgccgcc gaaggccgcc cccgccaagg cccataccat gaccaaggct
    28861 accaaaactc tctgacaccg cttgttcatc ccaccatgcc tccttagttt tcgtgctgga
    28921 tttcccgggg aaacccgccc tcttacctta caacagacgg ctcctttggc aaaggggctc
    28981 tccgaaatat ttttttgttt gcgaaaggcc gggccaaggg ggtctcccct ttcgaaacca
    29041 aaggggtaaa gtctgtcgat tatacacttt cctccggaag gccccatacg gtatgcttac
    29101 ggcgtgccga gcgggggccc gccccatcga cgtacccagc cttgtcccct ggtagaaaat
    29161 aaggacacta ccggaaagat cgccgggagg tcaggcccgc cccgagaaga gctccacctc
    29221 cggggaggga aagcatacgg agggcccttc ttgagagggg tctccaaaaa gaaagcaggg
    29281 tacgggatga gcagaaaaga tatcatgctg ggcaacgaag ccatcgcccg gggcctggtg
    29341 gaagccgggt gcgaggtggc ggcgtcctat ccagggactc cgtcttcgga aattctcccc
    29401 gccgtggctc actgggccga ggagctgggg acccgcactg tggtggagtg gggcgccaac
    29461 gagaaggtgg ccctggagta cgccataggg gcctctttcg cccgcaagcg ctcctgcgcc
    29521 atcatgaaac aagtggggct caatgtggcc atggatccct tcatgagcat cgccgccaac
    29581 gagatggatg ggggcgtgct cctggtggtg gcggacgaac ccgggtgcta ctcctctcaa
    29641 acggaccagg acagccgcgc cctggccctt tttgccaaga tcccctgctt cgacccctgc
    29701 gacgccgtcg aggccaaggc catggtcttt gatgcctacg agatctcgga aaagtaccgg
    29761 gtcctcacca tgctgcgtcc cacgggacgg gtgaaccact gccgccaggc cgtcgagtgc
    29821 ggcgacggcg tgatccccgg ccgccccttg gaattccgtc gcatgcccga gggacaccgg
    29881 atcctcatgc ccatgaccat gcgaggggcc ctggaacgac acaaccggcg tttcgaagag
    29941 ctccgccggg agttcggcac ggccttcggc aagtacaact tcgaggtccc cgcccaaggt
    30001 ccggcgcgtc tggggatcat cgcctgcggc ctgtgctacg ccgtggtacg ggacgtgctg
    30061 aaggccctgg ggcgcacgga cgtggcgctg cttaagatcg gtacccccgt acctctgcct
    30121 ctggacctgg tccaggactt catcggacgc cacgactccg tcctgatcct ggaggagacc
    30181 tttcccgtgg tggagcagca gctcctcgac cgcaccaagg tccaaggacg atggaatggg
    30241 tgggttccca tggaatggga gctttcgccg gaggtgatca cctccatcat cctccgggcc
    30301 ctgggagagc ccgcagccct ccccgcctgg gaagccacct tggaagaggc tcgcaaggcc
    30361 ctgcaactcg gcagacgcgg cccccggctc tgcccgggat gcggccaccg gccgagctat
    30421 ctcgccatcc gtaaggccta tcccgactgc attctttgca gcgacatcgg gtgttatggt
    30481 ctggccatcg cccagaaggc cgtggatacc aacgtctgca tggggggctc catcaccgtg
    30541 gcctcaggca tcgccatggc cttccgggcg gaaggcaagc ccgcctcgcg gccgatcttc
    30601 gccaccttgg gggactccac gttcttccac atgggattgc cgggcctcgc cagcgcggtg
    30661 tacaacaaac accccttcgt gctggtgatc ctggataacg gcatcaccgc catgaccgga
    30721 gggcaagggc atcccggcgt gggaggaaag ctccgccccg gtgaaaaaag cggctccgtg
    30781 gacatcgaag gagccgtgcg aggatgcggc gtgacccacc tggtggtgca ggaaccctac
    30841 gacgtctccg gctccgtccg ggtgctcaaa gaggcctggg aacacgcccg caccgccgaa
    30901 gagcccgccg tggtgatcct tcgccacccc tgcatcacca tgctcaaggc tccccccacc
    30961 cctcgtcccg tgcgggtcga tccggacaag tgcaccgggt gccggtattg cctgacgcac
    31021 tttaattgcc cggggatcgg gtgggatgag aacaccaaaa aaaccttcat cgaccgtcgt
    31081 ttctgcgtgt cctgcggagt ctgcgtcgac gcgtgccccc gggacgccat cgtcttcacc
    31141 ggagaggagg cctaacatga agtacattgt ggcaggtatc ggaggccagg ggatcctctt
    31201 tgccaccaag gtcctcggcc acatcgccct gggaaagggg ctccccgtca tcggcagcga
    31261 ggttcacggc atgtctcagc gcggcggctc cgtcataagt cacttcaaga tcggggacta
    31321 ccagagcccc ctcatcgcct cgggggatgc ggatatcttt ttggccttcg atcagtcgga
    31381 ggccctgagg aactaccact tcctccgcga gggggggcag gcggtcatca acgtggagcg
    31441 ccccgccgag ctggcccacc cccggcttca ggactacttc aggcagcggc gcatcacgtt
    31501 gcacgccctg gacggcctgg ggttgatccg ggagcatctg ggagggaagt tcctctttct
    31561 gaacgtcctg atcctcgggg ccctgtgcgg cgctggcgtc ggcaacttct ctagggacga
    31621 gatgcgtcag gccgtgggag agctggcccc ggcccggttc cgcgaggaga acctcaaggg
    31681 cttcgacctg ggctacggag ccctggcgaa gtagcgataa agaccgaaag gagatggaag
    31741 acatgaccac cgccacgccc caggcccccc ttagggtttt ggccatcaac cccggcgcca
    31801 cctccaccaa ggtgggagta tttgaggggg agacccccct gctgaaaaaa agcgtagaac
    31861 actccaccgc cgacctggcc aagttccccc gggtggcgga tcagtacaac taccgctttg
    31921 agctcgttga ggccgccctt cgggacgcgg gggtcgccct ggactctctg gacgccgtgg
    31981 tgggccgggg gggtctcatg aaccccgtcc ccggaggggt gtacgaggtc aacgacgcca
    32041 tggtggagga ccttcgcaag gcggagcatg gggagcatcc ctcgaacctg ggggccctcc
    32101 tggcccgaga attcgcccgc aaggcggagg tgcgggcctt catcgtggac cccgtggcca
    32161 cggacgaaat ggaacccgtg gcccggatct cggggctcgc gggcctcgac cgcaccggct
    32221 tccaccatgc cctgaaccac cgagcggtgg ccaagaaggt ggcccgggaa atgggcaagc
    32281 gctatgaaga tctggatctg gtggtggccc acctgggcac ggggatcact gtggcggccc
    32341 accgccacgg gaagtccgtg gacgtctgcg acgcccgctc cgaagggccc ttttccatgg
    32401 accgggccgg ggggctcccg gcctattccc tcatgaacct gtgctactcg gggaagtaca
    32461 cccacaagga gatgcgggag ctactcattg cccggggggg catgttcagc tacctggaca
    32521 ccaaggacct gcgccaggtg gagcgcatgg cccaagaggg ccacgagcag gccgccctgc
    32581 tcatcgacgc tctggcctat caggtggcca aggaaatcgg cgcccaggcc accgtgctgg
    32641 agggacacgt ggaccgcatc atcctcaccg gcggcatggc gaactccgcc ctcctcgtgg
    32701 aaaagatctc ccgccgggtg gcctttatcg cccccctggc cctggttccc ggagaagagg
    32761 agttggaagc cctctccgcc ggagtgcagc gggtgctccg gggagaggag acggaaaaaa
    32821 tttatcgcta ggggtgaagc tctccaccgc ccccccgggg gcgagaatca cctcgggcgt
    32881 ttcccaggcc ccccatgggg ggcctttttt ttatctccgc caggacgcgt tagaggggac
    32941 aaaaagcgtc ccttcccgcc ccaccggagt ctccagggcg tataatggac ccgccctgtc
    33001 ctcgtccgca gggacgttgc cccctttcgg aggtgccccc atgcccctgg tgctcgtgat
    33061 ggaccacaac gcccccaccc tgatccgatc cctggagcgc gagggattca aggccctcat
    33121 cgcctcgtcg gaagagcgag tgctcgccgc cgcccggtcc gccgatccct ccccggacat
    33181 cgccctgatc gacctcggcg ccaccggagg cggcttcgcc ctggcggaga agatccgccg
    33241 cctgcgcccc ataccggtgg tcctcctggc caacgccccc gaaaggcctt tgctggacaa
    33301 gcatcgtaac ggggaaatcg ccgggtgggc ctgccgatta ggggggcctg ccctggtggc
    33361 cgccaccgtg gatgcggcgc tgaaatcctc cctcgccgcc acccgccttt cctcggaaag
    33421 ccaagactct ctttccgccg ccctcttatc ccacgccgcc gagggcatcg tggggtacga
    33481 ccaaaacctg cgctaccgct cttggaacgc cttcatggag aagttcaccg aactcagcgc
    33541 ccgggaggtc atcggcaagc gtcccgccga gctcttcccc cccggggccg agggccccat
    33601 ggagggcctc cgggaaaggg ccctgaaggg ggagaccgtc tcgttccccc cccagccctt
    33661 ccacgtgccc cagaccggcc ggtacgggtg gatctccggg gtctacaccc cccatcggga
    33721 cggccaggga catatcatcg gcgtgctggg tttttttaaa gacgtgacct cggagcgacg
    33781 ctcccgggag ctcgtggagc tcctcctcgc cgaggccgac gcggggctat gggagtggat
    33841 ccccgccacc gacgccctca ccgtcaacga ccggtgggag gccctgctgg gctatgcccc
    33901 gggagagctg gccctcctat cccccacgtg gaaaagcatc gcccatcccg aggacgtacc
    33961 cccgatgcag gactccctcc gccgccacct ggcgggggag actcccatct atgagggccc
    34021 cctccgcctg cgggccaaag acggcgaatg ggtctgggtc ctagcccggg ggaaggcgac
    34081 ggagtgggac gtccaaaaaa agccctgccg cgtctcgggc accatcgtca acatctcctc
    34141 ctgcaaagtc agcgaggcgc acctccgggc ccaacgcgac ctggccctgg ccctggcgtc
    34201 ggtgaactcc ctcccccagg ccctggagct ctgcctggac gccgccctgg gcgccgcgga
    34261 gctggacgcg ggggctctat acttcgtaga acacgacgga cttcatctgg tccactccct
    34321 ggggttggcg gaggaagtgt ccctcgaacg gcggcattgc ccccccgaat cggagctcca
    34381 tcgcctggtg atcgcgggca agggagtctt cgtccctccc cgcaccctgg gactctcccc
    34441 caacgccctt ctacaggagc gcgacgggtt caaggccctg gccatcgtcc ccttcggcgc
    34501 ccagggaaag atcctcgggg cttttctcct gggctcccgt acccgggaga cctggacggg
    34561 gaagatccct tccctcttag gcaccatggc cgcccaaatc gggcccgcca tcgcccagct
    34621 ccgggcggaa gaatccctga ggaaaagcca ggaagagctc caggggctct ttaatgcctt
    34681 agaggatttt cttttcatct ttgatgatcg gggacaagtg gtctgggcca attcgttggt
    34741 cttctcccgc ctggagtacg ccgaggaaga gctcctgggg caacccctat cgcgcgtcca
    34801 cgccccgcag gacgcctccg aggcgccgga ggtcttcgac aagatcctgc ggggggagat
    34861 aaatctctgc accttggacc tgctcccccg ctccggtgat cccatccctg tggaggcccg
    34921 gttttcccgg gggctatggg gagggaaggg ggccttcttc ggagtgagcc gggacatctc
    34981 ctcccgacgc caggcggaaa gggcccttca tcaggccaac gaagccctga ggcagggttt
    35041 ggaccagctc cgccagaggc atcgggagct ggcgatcctc aatgacctga gcaccctcct
    35101 ccagacctgc ctggacatgg atgaggccaa aagcatcgtg gcccaacaga tggccctgct
    35161 cttttccgag gaatcggggg ccctgtacat ggcggtcccc ggaaaaaaca tcctggagcg
    35221 caccacggtc tgggggcccg ctcctcccga ggagtttttg atcagcatgg aagattgttg
    35281 ggggctacgc cggggaaaaa gccacctcgt gccccggggg gcctcggcga cccgttgtcg
    35341 ccatacccct ccccgggagg acacgagctc cctgtgcatc cccgtggcgg tccaggggga
    35401 aaacctcgga cttttgcacc tgcgccggga cagcgccgaa ttttccgaaa gcgggctcca
    35461 gctcgcccag accctggcgg acaccatggg attggccctg gccaacctca agctccggga
    35521 gacgctgaga cagcagtcca tccgagacat cctgacccag cttttcaacc ggcgatacat
    35581 ggaagagacc ctggaccggg agatcttccg ggcccagcgt acggagcgcc ctttgggaat
    35641 aatcatgctg gacatcgacc gtttcaaaga catcaacgat cgattcggcc acgccgtggg
    35701 ggacgaggtg ctgcggcagc tggcccacct cctccagaac tccgtacgat cgggggacgt
    35761 ggcctgccgt tacggcggcg aggagttcgt cctgatcctc cccgaagctt ctctggccca
    35821 caccgccgcc cgggccgagg agctgcgcca ggcggcccgg gccatcgcca tccccgcccc
    35881 cggggccgaa ggggagaccc tcgccatatc cctgggggtg gcggccttcc ccgaccacgg
    35941 cccccgggga gaaaacctcc tcaaggcctc ggacgacgcc ctatatagcg ccaagacctc
    36001 gggacgggac cgggtggtgg tggcccccat ccccctccag ggcggctcct ccgggatccg
    36061 ggcctagggt cgggtcccgg caccccagcg ccccaaacgg cggagtcggg cctcccctaa
    36121 atcaggaggc ccgactccgc cggtcctttc caagcgacgc agaagatctc gggaaaaagc
    36181 tcgccccgac cttcgcgccc cgccttaccc ccgcccccgg gagtgggaca aaactctctt
    36241 tccctgggag ggaaggggct cctggggggc atcctcttcc accgtgaagc gctccaccag
    36301 ttctcccaat tccgaggccc tttcgtagag gatccccgcg ttttccgcca ctcgagccac
    36361 cgaagaggag gactccgccg cgatggtggc gagccgctcg gcctcgccga aggccctgtt
    36421 cgtgaaggac gaaatgcggt cggtggaagc cgccacttcc tcggcgctgg cggactgctc
    36481 ttcgctcacg gcggcgatgt cttgcaccgc gtcggcgatg cccgccacca tctccaggag
    36541 ccgatcgagc cccgatcgca ccccccgcac cagctccacc gcgttttgca ccgaggtccc
    36601 tgtggtggcc atggattgca ccgtctcccc caccgcccct cggatccgcc ccaccagatc
    36661 ggcaatgctc ccggcggtcc ggttgctctc ctccgccagc ttgcgcacct cttcggccac
    36721 cacagcaaat ccccgcccgt gctcccccgc ccgggccgcc tctatggcgg cgttcagcgc
    36781 caggaggttc gtctggtccg cgatttgccc tatggagctc acgagctccc cgatgcgcag
    36841 ggcaaagtcc tgcacctcgt tcaccttggc gctggtcaac tccgtggcgc gagccatctc
    36901 ctgggccgcc tctcccagtt gagatacgct ccctgcggcc tggtgggcga aggatcgggc
    36961 cccgtcggcc ctctccgccg ccttgccgct ggcctcggcg gcggtctggg tgcccgcagc
    37021 cacctcctcc atccccgcat ttatggattc cacagcgctg gagttctccc ccaccacact
    37081 ccgcagcgcc gtgctggtgg aagatagggt ttccgccatg gccgccaggc ctcgggaggc
    37141 ctcgctcacg tcatccgtcc gccgttgcag ctcctttgcg ttgcccttca cccgtcccac
    37201 caggctcgaa agggtatcga ggaaggcgtt gaaattcgtc gccacctcgc cgatctcgtc
    37261 ttgggtcgtc accgggagcc gccgggtcag atccgccgac cctcccgcca gctcccgggt
    37321 catggccagg agccccctta ggggaaccat cagcgaacgg ttcaggaaaa acaccgcccc
    37381 ccaggcggaa aggagcacca tgccccccac tgcggccagg gcccagcggc gcaggtcggc
    37441 caaggccgcc aatccatctc gagcgctggc ccgcaccacc accgccccaa cgacttcccc
    37501 aaaggagttg acgaaggggc tcgccagcaa cacgtcctcg ccgtgccaca cgtaactatc
    37561 ccgtttttcc atgatgccac cgaagagctc cgccgggaag cgctccggcg aggccccctc
    37621 ttccgtggtg gccaccaggg aaaagcccgc cacgtcaaag gaggcggcaa actccatctt
    37681 cttcacgtca gccgccgggg caaacacgga aaaagaggcc cccgcgatct ccgccacccg
    37741 acgcaacacc tgcttgagct ctatgcccac ctcgaagctg ccgatgagac gctcttcttc
    37801 catcacagga aggatccccc ggaggtaaaa gccgaattgc cccagctcgg gccccgtaaa
    37861 gggcttctcc tggcgcagca ccgccacgag ccccgtgcga aagagaaggc tgtctccgaa
    37921 cttcttgctg ttcatgcgaa gaaagctcgt cacgtcgggg aggtggaatt gcatttgtcc
    37981 tatgccatga cgcccgttcg ccgccttcca cagggggtcc accagggcga aaagcccctc
    38041 ccggtcccgg gaggcgaagg cccggcgcac ctcggggaga gccacgacgc cgtccgccag
    38101 ggtcagagcc cggtgggctc cgtcctgcca gaccgcctca aagagcgacc gatccatttc
    38161 cgccacccgg agcacctctg cctcgatgcg atccttctgg gacaggtaag acaccccacc
    38221 caggatggcc gccagcgcca acaggagaat cagcacgccc cccgtcacct tgaccttcag
    38281 ggaaagcccc ttttttgcca tcatgacacc ctcctaaagt tatcccccta agacgcctca
    38341 tcctccccgg ggctttcggt ctcgccgaaa aagaagcggt ttggattccc cgcccggtcc
    38401 ccggaccatc cttcgcgccc cgcctcgtcc cctaggggcg tcgcgccttg gcccggggcg
    38461 agctgggacg cttcctcagg agatagcctc ccaccagggc gcagagggct cctccccccg
    38521 ccgcagcggc ccgtgctccg cttcgccctt cggcgatggc ctcctgggcg ctccgggcgt
    38581 cgacccgcac caccaccgcc cccaccactt cgccaaagga attcatgagg gggctggccc
    38641 gcatcccctc gccctctcgc ctcaggtaac gagaatcctt ttccatgatt cctttgaaaa
    38701 gctcctccgg aaagcgttcc ggggaggcgt cctcctccgt ggtggccacc agggaaaatc
    38761 cccccacgtc aaaggatgcg gcaaattcca tctttctcac gtcgtccgtc ggggcaaaga
    38821 ccgagaaaga ggcctccgtg gcctccgcca cgcgatggat caccggccgg agctcgacgc
    38881 ccacctcgaa gctgccgatg aggcgctctt cttccatcac ggggaggatc ccccggaggt
    38941 aaaagccgaa gatgcccagc tcgggccccg taaaggtttt cccctgggtc agcaccgcca
    39001 cgagccccgt gcgaaaaaga aggctatctc caaatttttt gctgttcatg cgcagaaagc
    39061 tcgtcacgtt ggggaggtga aattgcatct gccccacgcc gtagctctcg ttcgccatct
    39121 tccacagggg atccaccagg gccgccaagg cccctcggtc ccgggaggca aagacctgga
    39181 tcaccccggg gaaagcccgg atacgatccg ccaaaatcag cgaccggcgg gcctcgtccc
    39241 ggcaaaccgc atcgaagacc tcctggtcgt tttccgccac ctggcgcagc tccgcttcta
    39301 tgcgttcctc ccgtagggcc aggaaattcc tgccccagac gatggaaata cccatcaaag
    39361 tcagtaaaag cacgcccacc atcatccggg tcctcaggga aagccctcct ttagccatcg
    39421 ccaccccccc cttttcccca ccgggagcac ttcggcccat tctaccccgg acccaggggt
    39481 ccccacaagg gcggaggggc cgacaaaaga ggcacgaaaa agcatgaatg acctaaaaag
    39541 cgccgaaggc cccttgcgaa ggggtgggaa tctccggtac catggggtat ccggtatagg
    39601 ggtgcgaaaa ttacaaggtc gaaggagggg aattcccatg cgctttaaag ttttagccct
    39661 gatgtgcctg ggattatttc ttgcggtgcc agcggcggcg gaatccatca cggtgaaaat
    39721 ttgctacaac ggtccccctc agccggggaa caacgcggtg cacgccttcg tgctcaattt
    39781 ccagaagttt ttggaagaag gcaccgcagg acgggtgaag ctggagctct atcccgacag
    39841 ccagctcggt agcgaagaag agcgcatgga acttctctcc cgcaagggca tgaaccagcc
    39901 cattctcaac gtggcgtcct tcgccggcgt ggccccggtc tttccggaaa tctacgcctc
    39961 ctccctcccc ttcctcttcg acaaccaccg ggcggctcac atcttcttcg acgaagggct
    40021 ctggtggaaa aaggctcagg aagccttccg ggaacgcacc ggcgccgtgc tcctggaggc
    40081 ggtggaagag gggggatttc tcgcgttcac caacgcgaaa cgggaaattc gcacccccaa
    40141 cgattttgaa gggctcaaat ttcggggcat ggacgagggg cagatcatcc tctacaagag
    40201 cttcggcgcc agcggcaccc ccattccctg gacggagctc tatctcgccc tgcgcaccgg
    40261 cgtggtggac ggccagatga accccgccat gtacgtgctc atgggaagcc tctacgaggt
    40321 gcaaaaatac atgacgctgg cgaacatcca gtactcggac cagttcctcg tggccaacgg
    40381 agacctcttt gactcccttt cgccggagga ccaaaaggcc ctggtggatg cggccaagcg
    40441 ggccaacgaa atcagccgcg ccgaggtgga ggccgccgac gccgcccagg tggagcaact
    40501 ccgggccaaa gggatgcaga tctacacccc cagccgggaa gagctggccg tcttccggca
    40561 aagggcccag aagccctatc tcgcctggct ggagacaaaa gtggggccgg aggagctcac
    40621 cctcgccctg gacagcgcca aggccgccaa cgaccgggcg gcgggggtct gccccttgag
    40681 aaactaaccc gtcggggggg ccgcccggga acnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    40741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    40801 nnnnnnnnnn nntttttcgt accatggcca caccgcaccg gagaaaggac gtggcaccat
    40861 ggggccccac cgaagcacgg cggcatttcg tgaggttttc gtggaatccg ggggccccct
    40921 tggggaaacc ggaggaaaaa gatgacggcc atggcagacc gtctccaaag gatctcccgc
    40981 accctcgaac gggtgagcgc cgtgggggcg ggggcccttc tggtggtcaa cgtggccgat
    41041 gtgctcctgg gcatcgtctg ccggtatgtt ttccgcatct cgctggtctg gaccgaggag
    41101 gtggcccggt actccctggt gtggctggcg ctcctggggg cggggtgtgc cgccgcccgg
    41161 ggagatcaca tggcccttga ttttctggtg ccccgccttc cccgggctcc gcgcctcgtg
    41221 gtggaggtcc ttcgcctcgc cttcgtgctg gcggtgcttc tggcgctggc ggtgctgggg
    41281 tggaaaaatc ttgcgggcac ctgggaagtg aagaccatgg cgctgggggt tcccaaggcg
    41341 gcgttgctgt tggcgctgcc cgtggggatg ttgttgctgg ggctccagct ttttctgggg
    41401 gccttgctcc cccccgaaaa ctccgaaagg accgaaggcc catgatgtgg tgcgccctgc
    41461 tgggggcttt ctttctgcag atgcttctgg gcattccgct cttcgtgggg cttgtggcca
    41521 cggcctttct gggggtggca tctctcggga acgaaagcct cttccgggtg attccccagc
    41581 agttcttcgg gggcatggac gtcttttctc tgctggccat tccctttttc ctcctcacgg
    41641 ggacgctcat gaaccgcgcc gggctcacgg accgcctcat ggatttctgc cgtctgctgg
    41701 tgggatccgc ccggggaggg ctggggtacg tcaacgtggt ggggggcatc attctggctg
    41761 gggtgaacgg ctccgccgcc gccgacgcct ccgcgctggg ctctctcctc attcccgcca
    41821 tggaaaaaga gggctttccc cgggcctacg ccgcgggact caccgccggc agcgccctca
    41881 tcggtcccat cattcccccc agcattttca tggtcatcta tgcttccatg accaacgcct
    41941 ccatcggagg gctcttcgct gcgggggtgg tgccggggtt tttgctggga ggggccttcc
    42001 tcgccatgaa cgccctgtgg tccgcccgat accgctttcc cttctccgac ggcgctgcgg
    42061 tgcgggcccg caagggggag atcctgaagc gatccaccgg ggctctggtg gcgccgttgc
    42121 tcatcatcgg gggcatcgtc gccggcgtcg tcacccccac ggaatcgggg gctctggcgg
    42181 cggcgtactg cgccgccgtg ggattttttg gcacgcggca actgaccctc cgggggctgg
    42241 gagaagctct ggtggaaacg gtgcgcctca ccagcgccat ttttctcatc atgggagccg
    42301 ccacctccgt ggggtggctc ctcaagtggg aacgggtgcc ccagacggtg gccgccgcgc
    42361 tggcggggtg ggggcttctg gacacccccc tgctgctgct tctgaccctg agcgccctga
    42421 ccttcgtcat cggcatgttc atggaagagg tggcggtgct cacgctcctc acccccatct
    42481 tcgcccccct cgccgcccgg gcgggcatcg accccctgca cttcggcgtc gtcatgaccc
    42541 tcaacgtcac catcgccctc atcacgcccc ccgtgggggc ctgtaactac atcgtggcgg
    42601 cggtggggcg ggtgcccctg ggggaactct tccgggagat ctggcccttc atcggggtgg
    42661 ccgtgggcac gctcctcctc ctcatcctct gtcctcccct caccaccgcc ctgccccggg
    42721 ccctggggct gtgaggcccc ggagggcgcg aaatcccagg gacgcatcca agggcctttc
    42781 tcttcgagca tccaaaggcg ttttcccttt gcttcgccgt ctccctcgtg tatcatgggc
    42841 cccacgagcc ctccggcgtt tctagggctc ccgggagcgt gaaagatcat atcacccgca
    42901 ttgatcatcg tcttatgtct tctcacggcg gtctgttggg ccgcttcacc ccttttgctc
    42961 atggtgggga tgcgaagcgc gaacctctac gaggtcaact tcatccgagc cctgggattc
    43021 accggggtgt gcggtgcatg gcttttggcc accgacccct cggcgatgat ctggcaccat
    43081 cccctgtccc tcctgggggc cgtgggggcc aatgtgatgt tgggcaacat cctgggagat
    43141 ctgctctact ttcaatccct ttccgatatc ggcgtgagtc gcgccgccgc catcaccagc
    43201 acttaccccc tggtggcggc gggcatttcg gccttctggc tgaaagagcc cctcaccatg
    43261 cccctgatcc tggggacggc gagcatcgtg ggggggctta tcctgcttcg cctcgaggga
    43321 acgtctgccg acgcccaagt ccgggcccac cccgtgcggg gctttctgtg ggccctcatg
    43381 gcggggattt tttggggcat gggcatcccc atcaccaagt ggttggtagc ctcccaacag
    43441 gtgcccccat ccaccgtcaa cctctggcga tccctggtgt tgttgctctt ttccggcgcc
    43501 ctatggctat ggccccgccg aggaaccccc ctggggagcc gattccggcg cctggggacc
    43561 atccccccgg cggggtgggt caccatcctg gcctcggggg gagtgagcct cggcgtggca
    43621 ggtttcatct tcatctccgt actgcgattc gcccccgcct ccatggtgac tcccctcacc
    43681 tccgtgaacc cgctcctctc caccctcttt gcggtctgct tcatgggaga gaggttgcgg
    43741 cggatccaat ggatcggctc ggtcctggtg gtggtgggat cggccgctgt ggggatgtga
    43801 ccgaaagggg tggcgggggt cgccccgggg cccccgaggg ggcaagagaa ggaactgggc
    43861 ccggatcact tccgcgaggt ctgcggcggc accgtcctcc gtcgcaccat aacgccacaa
    43921 aaaaactccc cggaccttgg gcccggggag tttttcgcgc gcaggagtcc aaaaggggta
    43981 atctaaatct acttaccctt tttcgtcgcc ttgggggcgg gggccacctt cttcaggtag
    44041 acgttgccct cggggatgaa gtgctctccc tcggggggag atacgatctg gaagagcatg
    44101 atgtcttttt tggggccctc cagccgcatc atggcccgac cctcggcgtc gcaggcgtag
    44161 ctgcggatct tcaccaggat ggcgccgtcc acgaagttgt cggagaagat gctgatctca
    44221 ccattgctgg aatccacttt ggctccgtct tccgccgtgg ccttgtggct tcccttcacc
    44281 aggatggggg gcttaccctt ctccttctgg accttctgag ccagctccaa caccagggtc
    44341 ttgccgtcct tctcccactt ccacgtgccc gaaaaatcgg gcatggccgc cgccgcagcc
    44401 atggaagcga aggccaccag agccagcacc gccaccaccg ataccactgt cttccgcatg
    44461 tacttcactc ctccttcata gatcctcgtc gccttccgac gcttggagat attatcacgg
    44521 ggcggccttt tttgccaccg ctctttggct tatttcggag agaaaaggcc ggggcgaagg
    44581 gggggcgggg cgagatccgc aggcctttgc ctttcccttt ttcgcccacc gccctcgatt
    44641 cttttttggg actcgcgcta taatcccctc taggaggggg gatttcccgg tgaaggtcaa
    44701 ggcaaatcgt caggtcctaa agggggccct ggggatcggg gcggccctgg ccctgggggc
    44761 cgctatcctg gtggcgtcga cgccgaagga ggcggtgtac gtcttgtgcc tccaccacgt
    44821 ggcccccgtc cccggggagg acggaggaat gtatgtttcc cccggggagt tcgcctcgct
    44881 attagcccgg ctccgacaag ggggggcggc ctttctctcc ttggaggagt tccgccggtg
    44941 gatcgagggg accggtccca tcccccccgt ttccgtgctt ctcaccttcg atgacggcta
    45001 tgaggacaat gcctcggagc tactgcccat ccttcggagt ttcgacgtcc ccgctgcggt
    45061 cttcgtggtg ggggccaacg tggggacccc ggggaggctt tcggcgcccc agatgcgggc
    45121 catggcccaa agcggcctgg tggcctttgg cagccacacc cagcaccacc gggacctccg
    45181 ggggctgtcg gcggcccaac tcaccgaaga atttgccgga tctcgggcct ttctcgaagg
    45241 ccttctcgag gtctcggtgg acgttttgtc ctaccccaac ggccgcaatg gccccgccat
    45301 ccgccgcatg gcggccaaga gcggctacgc cctagccttc ggcacggagg gggccccttg
    45361 cacccggagc tccgacccca tgaacctgcc gcgctacacc ccgggggcct tctcccgggc
    45421 cctggggtgg cgtctggcga tccgacgtct gttggagtga ctccggccta ctccatcccc
    45481 gaggcgtccc gcacccgacg ctccagctct tccaactccc gcaccacgtc ggcgggcagg
    45541 gcacgccaga gtttttcccg ggcccggcgc cccagctcgt cccgggaaag gcctcgctcc
    45601 ctccccaagg gcccccaacc gatcttctcc cggccgcagt agaggtccat gtcgaggatc
    45661 cgttcccccg tggcgtcgta ctccagtccc atgaggaact ccttcaccac ggaaggcgcc
    45721 aggaaggtga gcagccggat gcggtaaacc cgcaccacga gcagcatcct cgccgcctct
    45781 cgggtccatc cttcgctgaa gggaaagccc atttccaggg cgtcgagcac cagctccacc
    45841 cgacggatgt gctccggata gaggtcgtac ttgtcgtaga tctgcacctg gacctcaccc
    45901 caggtcgcgt cccccaggct ctggctgaag agctcgaagg cccgggggcg gaggcgccgg
    45961 taggccaggt caaagggcag gtcgagcacc agcacttcgg ggtgcaggtc ttgccacaag
    46021 acggggcggt tcagagggcg agaggtgcac cccaccaccg ttcccgagcg gaggcgccac
    46081 tcgaagaggg catccaagga cgccaccgtg ccccccagct cccggcgaat gtcttccagc
    46141 acagaggatc ccttcccgaa ggaccggagg aagatccacc ggggcaggag ccgggattcc
    46201 ttcaggcccg aatccccttt tacgttcagg agggcacaaa aaatctctgc gggcgaggcg
    46261 ttacagagcc ccacggagag gtctcccccc gaggagtcct ccacgtggag cacggtctgg
    46321 tcctcccggg actggtccac cggatcgaca agcccaaagg tcgcctcgaa gttatggatt
    46381 aaaaagctcc ggagggcttc cccttctcct cgtaacatca gaaagtgcgg tccactgcgg
    46441 atgagcgaca gcatcgccat tcctcctctc tgagtcttac cctgcgagcg acggcgccgg
    46501 accgggccac cgccctccaa gtcccgggcg tccctttccc gcccggatgc cgttttaggc
    46561 ctcgaaatct tcggggggat cggcggcggg gagggccatt tccacccgca acccccctcc
    46621 cgaggggttc tccgcccgga tgatgccccg gtgaagtttc accgcccggg cagcgatggc
    46681 gagccccaac cccgcccccc cctctccggt gcgctcccgg gcccgctcgc tcctcacgaa
    46741 gggacgaaag atactcccca aatccccatc ggggacccca gggccccagt cccgcaccgc
    46801 caaacgcacc ggccgggccc cggagggttc tcgccggagg tcaattccca ccgacgtacc
    46861 cccgggggcg taacgcaggg cgttgcggac cacgttctcc agagcgctgc gcagcaggga
    46921 ttcgtcccct cggacccata tgccgggggt cagatggggc tccacttgct tccctccgct
    46981 ttccgcctcg aaggccccat cttccgccac ttgcctcacc agggtactca ggtccaccag
    47041 gtcgttccgg gcggggcgat tcatcatgtt cagggaagaa agggatagga gggagctcac
    47101 cagctcttcc aggcggtgga gctctcgctc cacccgatcc aaaggcgccg ccccttcggg
    47161 aagctgcttt tgggccaggg ccagggccag agaggcccgg gtgagggggg atcgcagctc
    47221 gtgggagacg tcccccagga ggcggcgctg ggacaggagg agcccctgca tgcggtccgc
    47281 catggcgttg aagtcttcca tgagggctcc gatctcatcc ttgtgacgat aggcctggcg
    47341 aggggcccgg gccctcatgt ctcccgaggc gaagcgtcgt accgcctccc ggagttggag
    47401 cagggggcgg gagatatgat gggcgagctg ccaagccatg acccccacca ccagaagcac
    47461 cgcccccacg cgcaggatca gggcggggag agcgctctgc caggagggcc attccgcctg
    47521 gagcaccagc acccccggcg gattctccag gggcaccacc agcagggtct gggtgccccg
    47581 ggcgctccgg gaggggctcc gggaggtgag ggtcttctgg gcttcccgtc gcaccatgtc
    47641 cgacaccgac cgccccaggc gttcctcccc tgtggcgtcg aagagaaatc cccggatgcc
    47701 cacagtggtc tccagcaggt cgagccgggc ccgggccgct tgggcgccgt gctgacgcca
    47761 cagatctccc aatccctggc cgaagagggt agcggcctgg aagacccccc gctcccagcg
    47821 ttcggcggga agctccggtg ccaaggtccc cgtgaccatc tgaaaggcca ggagggccac
    47881 cgccagggta aaccagaacc agagaaggag tctcagaaaa aaccgggaca tgcgaacagg
    47941 tacccttccc cccgcagggt acgaatacgg gggcttccgt cggcaaaggg acccaatttg
    48001 cgacgcaggt tgctcacgtg cacgtccaag ctccggtcga agggctccag ggggcggtcc
    48061 agggccgccc gggcaaggcg atcccggggc accacccggc cgggggagcg catgagggct
    48121 tcgaggagtt tgaactccac cccggtaaga tccacctctt tcttgtcgca tcgcacggac
    48181 cgagagctgg aatcgagcac cacgtccccc accgcgaggc gggtcatgga ttcccccctg
    48241 tccccgtggc gtcgcagcac ggcccggatg cgggccacca gctcccgggg gttgaagggt
    48301 ttgggtaggt agtcgtccgc ccccatctcc agccccacga tgcgatccac gtcatccccc
    48361 cgggcggtga gcatgagcac cggcatggtc gaacgacccc ggagctcccg gagcacctca
    48421 aagccgttgc ggccagggag catgacgtcc agtaacatca tgtcgaaatc cttctccagg
    48481 gcccggacaa ggcctccggc gccatcgtgg gcgaactcca ccgcaaagtt ctccgaagcc
    48541 agatactccc gcaggagctc acagagctcc acgtcatcgt ccagcaagag gatcttccgc
    48601 attttccacc ccctccttcc gggatgacgg ctcctccgcg aggcacgagg cgcagaggcc
    48661 agaaaccacc agctgcttgt ccgtgacccg ggccccgggg ggaacggcca ccgtgggcca
    48721 gggttgcccc tccaggcacc acatgcgacc gcaggcggtg caaagaaagt gcggatgccc
    48781 cccgggacac cggggggtct cggcgacgtg ggcccgaaag cgccagaccc cgtcggtacc
    48841 ctggatgcga tgcaccactc cggaccgctc caaggcctcc agggcccgat agagggtcac
    48901 ccgatcgccg tgaggcatcc gggccaggag ctctccgtgg ctcaagggac cttcggcccg
    48961 gcgcagcagt cccagcaggg cctcccggaa gggcgtccgg cgcaaccccg cccggcgcag
    49021 cgcttctcgc tcggggaacc cctcttcatc catcgctcct gcccccttat cnnnnnnnnn
    49081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    49141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngaacagggg gccccctatc ccctcttcgt
    49201 cagcgccaac gaaaaatggg cctcgtacct ggaggaaaag ggccttctcc gggagaagcg
    49261 ccctgtggcc cagagcgccc tggtgctctg gtggccccag gacacggccc cttctctaga
    49321 cctgctgaaa gatccccaga aacgggtggc cgtggccaat cccgacgtgg cgcccctggg
    49381 agtggcggcc cgggattacc tgcaaagcga ggggctctac gacgccatgg aaaaagaaaa
    49441 acgcctcatc ctgacgtcgg acatactggg agtggccctg gcggtaaaaa gccacggcgc
    49501 cgacctggga tttctggccc agggcacggc gaaaaagtta ggaggttcct gggcttccct
    49561 ccccggcaag ccccagcagt acgtgggggg gctcacggct ccggagagct ccgaagtcat
    49621 ccgagatttt tggagcttcg tcaccggccc cgaggggggg cgcatcctgg aagaagaggg
    49681 cttcgaagtc gccagaccct gacttcctca ggcagagtag ttccggaaat ctctcgggcg
    49741 ggccgaccgg cggcgcgccc ttttttgtcc ccgccctccc gagggccccg ggtgaagggg
    49801 ggctcgccct taggcgatgg cgccttttta ctcccagggg atctccccga tcgccaaaaa
    49861 agtgctataa taaaaaattg ctccggccat gccggtttgc cactctccgg ggaaaggcag
    49921 aatcaccacc catgatcact cccgataaga tccgcaacgt ggccattgtg gcccacatcg
    49981 accacggcaa gaccactctc atcgacgcca tcttccgcgc cacccgcacc ttccgggata
    50041 acgcccgggt gcaggagcgc gtcatggatt cgggggagct ggaacgggaa cggggcatca
    50101 ccatccgagc caagcactgc accgtcttct ggcaagacta ccagatcaac atcatagaca
    50161 cccccgggca cgcggacttc tccggcgaag tggaacgggt gctctccatg gtggactccg
    50221 tgctccttct ggtggacgcc gccgagggcc ccatgcccca gacccgatac gtcctgctac
    50281 gggctctccg gatgggtttt cgtcccctgg tgatcatcaa caaggccgac cgtcccaacg
    50341 ccaatcccca ggacgccctg aaccgcacct tcgacctctt cctcgacctg ggggccaacg
    50401 agcaccaggc cgacttctcc gtgctctacg gctccgccct gcagggatgg atggtgcgcc
    50461 acatggaaaa agatccccgg gaaggactcg atcccctttt tgagaccatc gtggagcgcg
    50521 tgccccctcc ccaggtggac ccccaggctc ccttccggat gcaggtgagc accctggcct
    50581 ggagcgacta catcggccgc atcggctgcg gcaagatcct ttcgggaaag atctccctgg
    50641 gggacccctt ctcccgcatc gccacccact ggacggacga gggacacacg gcctgggagg
    50701 tcacctccac caagatggaa aaagtggccc acctctgggc cacccggggg ctggaccgta
    50761 ccgccgtgga cgaagcccag gcgggggaca tcgtgtggat cgcgggcccc caggagatcg
    50821 gcatcgggga caccttcgcc gccccagatt ttccccaggc ggccctggtt cccctggaga
    50881 tcgaggagcc cacggtgtcc atgttcttcc tggtgaacac ggggcctttg gcagggcagg
    50941 agggcaaagc cgtcaccctg cgccaggtcc gggatcggct ggagcgggag ctaaaaagca
    51001 acgtctcgtt gcgcatggaa gacctgggga gggccgacgg cgtgaaggtc tccgggcgag
    51061 gggagcttca cctggccatt ctcatcgagg aaatgcgccg ggaaggaatg gagttctgcg
    51121 tctcccgccc agaggtcatc acgaagcgcg atgccgaagg gaacttgacg gagcccatag
    51181 agcagctcat cgtggacgtc cccgaggagt accaaggggt ggtcatcgaa aagctctccc
    51241 gacgaaaagg ggagttggtg ggagtctttc ccacggggcc cagcaccgtg cgcctggagt
    51301 tcaccatccc cacccggggg ctca
//
LOCUS       DAOOER010000009        47366 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_820_length_47366_cov_8.424024, whole genome shotgun sequence.
ACCESSION   DAOOER010000009 DAOOER010000000
VERSION     DAOOER010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 47366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47366
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_820_length_47366_cov_8.424024"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="PL162_02250"
     CDS             complement(<1..185)
                     /locus_tag="PL162_02250"
                     /inference="COORDINATES: protein motif:HMM:NF014849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HQF90884.1"
                     /translation="MIDTMRSFICLPLQHDLARRVESWIGELRRVAPRIRWVAGDQLH
                     VTLKFLGEVSFVTVAELA"
     gene            complement(182..697)
                     /locus_tag="PL162_02255"
     CDS             complement(182..697)
                     /locus_tag="PL162_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HQF90885.1"
                     /translation="MDRDRLWALAERLVALGTRQKKTLGLAESCTGGLLSGAITAIPG
                     ASSLFMGAVVSYSNGIKNSLLGVPEEVIDRQGAVSEACARAMASGARRVLAVDLALAV
                     TGIAGPDGGGPEKPRGTVWFALAEAQSERAWHRLFSGNRDEVRSQSVHFALERLVEAL
                     GSGAEGEGGGP"
     gene            complement(709..1203)
                     /locus_tag="PL162_02260"
     CDS             complement(709..1203)
                     /locus_tag="PL162_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HQF90886.1"
                     /translation="MIAPRRPLPRSWEGWVATAGGVGCFSSVPGTVGSGVAFLGASIY
                     PARHLPLFALLIVAVTLLGTWAADRYARRKGETDPSEVVIDEVAGMWVAQFGLEPSLA
                     LGSFLVFRILDIVKPFPIRQAEALPGGVGIMADDLLAGIATNAILRFFSWFFLAGGMA
                     TLLE"
     gene            complement(1200..2483)
                     /gene="rimO"
                     /locus_tag="PL162_02265"
     CDS             complement(1200..2483)
                     /gene="rimO"
                     /locus_tag="PL162_02265"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HQF90887.1"
                     /translation="MKVSIISLGCAKNQVDSERLASQLRGGGHLLVPPSEPSDLLVVN
                     TCGFIASAVRENVDLLLDVERLKQEGRLGRVAVMGCLVNRHGDELRRELPWVDSWWGA
                     GEGDALARTLGAQEEGKRDPLPEGLPWSRYLKIAEGCDHRCSYCLIPALRGPLQSASP
                     KEILRDAERLVASGAREICLVAQDLAVYGEDRGEAGLESLLKDLSRELPRDVWVRLLY
                     LHPSHLKESFWEHMLSLPQLLPYADVPIQHADERILEAMNRPARTEELRRLFLAARRA
                     SPDFALRTTVMVGFPGEDRRAFETLRDFLEEISFDRLGSFVFSPEEGTAAAALLPSVS
                     RRTADRRQERIMALQERLSLARQERWVGRTLAVLVESADPDEGLAWGRSFREAPEVDG
                     EVEIRSGSIPVTPGQVVSVRVLEAHPHDLVGEVAS"
     gene            complement(2493..3446)
                     /locus_tag="PL162_02270"
     CDS             complement(2493..3446)
                     /locus_tag="PL162_02270"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HQF90888.1"
                     /translation="MHEEGLSLKKLGEEAVRLREARGLSQKDVAEALHIRERFVDAIE
                     KGDEGCFPAPIYARGFIRNYLEFLGAGQLWKAFAEEVPLEQPDDLDTDSAVACSPLSS
                     GFRRISRRWVYLMVVGLAILTAGVVWKEWDVLRAQIGQGGRPDAASVDRALTPLPLLS
                     PTGAPTPAQRAALPGGSSDVGTSGASKDASIGDRVLSPEILSPEPHRSADATSEDYPW
                     LKEMNQIAKSQDAEGKNLILLASSPCWVRAVQGGRTVFETTMKAGEERRIPLTSPVRV
                     RMGKSSAITATWGGQTRAMGKGTGGGVATVEFFPDGTMEVK"
     gene            complement(3422..4156)
                     /gene="rpe"
                     /locus_tag="PL162_02275"
     CDS             complement(3422..4156)
                     /gene="rpe"
                     /locus_tag="PL162_02275"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HQF90889.1"
                     /translation="MAGSLPVSARVLRESGPLISPSLLAADPLRIGADLERLGDPWIR
                     WLHLDIMDGHFVPNLSYGPALLRALARDFSGGVLDVHLMVEPPERFIDAFLAEGPSVV
                     TVHGEATPHLHRVLQALRQAGVSPGVSLNPGTPVEMILPTLHLVDLVLVMSVNPGFGG
                     QSFLPEVLPKVHALVREREIRGLDFVIQIDGGMGESTVGAAAAAGCDVLVAGSALFGA
                     SDLGKAAETLHRVAQEARVCTKKAFL"
     gene            complement(4135..5286)
                     /locus_tag="PL162_02280"
     CDS             complement(4135..5286)
                     /locus_tag="PL162_02280"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HQF90890.1"
                     /translation="MRKLVPITLLLVLLVIVGSGVFTFYAVFLGGNDVSMPKVEGLSV
                     IDAVEILKKSELVARVDQLDSLKKPGTVLAQSPAEGTLVRKGKAVILKVSKGGRQVSL
                     PDVRGMEYEQAVQTLQAEGFTPGDVVRVADKNLPAGAVVAQSPASPAAVPAGMRVDLL
                     VSYGGGAPQGLVKVPELAGRPEKEARKMLEDGGLRVGGVRYQYTLATPPGLVVRMSPS
                     PGTKVSKGSRVELVVATADRAAGGKTPTPQGEGGTGGDSVGSPPGTVRVPVVGVPPRA
                     EGEEGVPPQVGPTVVAAPPKGEEPPKKATPAPEGGSRGKKAKIRYQVPPLVKPMNLRI
                     EVVDRNGTRSVLDKTVQGGEYIKLEETYAQEAVVTIYLGGEFVWQDRYQ"
     gene            complement(5371..6690)
                     /locus_tag="PL162_02285"
     CDS             complement(5371..6690)
                     /locus_tag="PL162_02285"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HQF90891.1"
                     /translation="MRGVEAALRLVEGVDRGEFLSEALRRLASEKIGTPEDLSLAATL
                     AYAVFRRKALWERLVREALTPPRRDPSPGAMRALVVGAAGLCTLRTFAPAALGNALVE
                     YVRRISPREGALVNAVLRRIEPRGISLMERLGASGALLDQALFRGVPDWAARRFEEDF
                     GPSGARQLVKMASIRPYTSFCLSPGESPQGWAERLGEAGLRAWPSPALSGVLRTAATP
                     HIPSVPGFLQGALSPQTESSMRVSELALSLAGDGPVLDMCAGRGGKTAHLISRLPAGA
                     VEAWEKSPGRCRAFRGEMARRHLVGAVIEGDALALEASRRYRLVLVDAPCSGSGTWGR
                     HPEAKWRVTPSEVGALAALQRRLLARGASLAASGGYVLYATCSLFREENEAVVADVLA
                     RDPSVVEVPVLTGKAPWRRGRPWGFSIFPALPWVDGFYVTVLMKVSA"
     gene            complement(6690..7397)
                     /locus_tag="PL162_02290"
     CDS             complement(6690..7397)
                     /locus_tag="PL162_02290"
                     /inference="COORDINATES: protein motif:HMM:NF039426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HQF90892.1"
                     /translation="MQGCAPLLVAFLALGLFLPGIIFLVLGAAAVFFLAFVPLGFAAQ
                     SLFWIASGPSQLYGILVNPRVRRNHALEHATLHVLEETYGPLNLSGLAFEKGFTVRGD
                     LFDGATVLGAARTALRRLQGGEAGLAIHPRCGTTAVVANLLAAVAFLLLLMITGRMSL
                     FSVLVALGAAQLVGPSLGVWAQRWITTDVDVSQLEILGVELQGRRRYGLGGVVIPEQL
                     LVVTRISGKDVYPEVVS"
     gene            complement(7437..8120)
                     /locus_tag="PL162_02295"
     CDS             complement(7437..8120)
                     /locus_tag="PL162_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HQF90893.1"
                     /translation="MFFPMDATLLLLIPAVILALWAQMRISSTFRRYSQVSSARGVRA
                     SEAARRLLDRFGLNHVAVERVPGSLTDHYDPRSKVLRLSDDVYGSTSIAAIGVAAHEA
                     GHAVQDLQGYAPLRIRNAIVPVTNLGSTLAMPLFFLGIIMRIPALMDLGILLFAGILV
                     FHLVTLPVEFDASSRALRLLGETQVLGTAEIAGARRVLNAAALTYVAATVMAAVQLLR
                     LLLLRGRDE"
     gene            complement(8438..9571)
                     /locus_tag="PL162_02300"
     CDS             complement(8438..9571)
                     /locus_tag="PL162_02300"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQF90894.1"
                     /translation="MATRSLPTLDLKRNYRRIREEIAEAMDEVLESQHFILGPAVGAF
                     EKEVQSYLEVPHAIGCASGSDALLLALMALDLREGDEVITTPYSFFATVSSITRLGGR
                     PIFVDIDPETYNLRMDQVLEKISPRTRAILPVHLFGQMAEVELLDPVLREGRLRLVED
                     CAQSFGAWRRIDGRLARSGSVGSLGCFSFFPTKNLGCYGDGGMISSTDDALATRLRRL
                     RVHGAEEAYFHQEVGLNSRLDTLQAAILRVRLRHLEEWNEERRLAAERYRILFEERGL
                     LDALTPPAEDPQNYHIYHQYVIRCSRRDDLQAFLGAEGITSRVYYPLGLHLQPCFRFL
                     GGQEGDYPEAERLSRESLALPIFPEITPEEQEWVVDTIRRFYD"
     gene            complement(9702..10355)
                     /locus_tag="PL162_02305"
     CDS             complement(9702..10355)
                     /locus_tag="PL162_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQF90895.1"
                     /translation="MLETQLYTTSADYVYRELRHRIITKKIKAGQRLPEVNIAVQMGV
                     SRTPVREALRRLESEGLVDIVPNSGARLASPSRREMESAYVVREQLECLAARLAARAM
                     SEKHFRRMEEIIGAEEEAFRDRNLELYMEINEAFHRTVAEATGNRILASYVENILART
                     NVYIVFYDPFYEDEVNPSMAEHKDLLRALRDRDGVRAELLMRDHLRTTMTGMAQPDH"
     gene            10646..11872
                     /locus_tag="PL162_02310"
     CDS             10646..11872
                     /locus_tag="PL162_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HQF90896.1"
                     /translation="MPLPTAALPREDVTLLPSPLIEFIRLGLEHSAIPLTSGQPHPSC
                     YPLTELATAASEAIRGEGERHLRYGANRGLGKLRALVNKELINRSLFPPDLSPDSLVL
                     TVGAQGAFDLLCQAVLRPGEAVALDAPCYPDTWCTVVRRGGKVIPIPVDAEGMDVDAL
                     EGALHQGIRPRLVYTIPTYQNPTGCSLSRERSSRLVALAERFDFLVVLDDPYRLLPLD
                     EPLRSGEEFFPWKGWDSGRVVALGSFSKILAPGLRVGWAHAAPELADALATLQEMSHI
                     SLPALDALAVASFLEHHGLEGQIQRVRTFLQGQRAALIDAMAEARLEELGCGTMASAG
                     GCFLGLVLPAGREASPVARTLAADFGVATVPERAFWPPHGEGTPDRFLRLSFSWATPE
                     EFRRSAQAFRQVLTTP"
     gene            11881..12099
                     /locus_tag="PL162_02315"
     CDS             11881..12099
                     /locus_tag="PL162_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90897.1"
                     /translation="MLVLSLGCVVAYGRALGELGGYFLGKGSLVVVGVGLLGGTLAGV
                     GAVGLWRRYLVPRDPSAPKGPPKVDPRL"
     gene            12204..13007
                     /gene="nudC"
                     /locus_tag="PL162_02320"
     CDS             12204..13007
                     /gene="nudC"
                     /locus_tag="PL162_02320"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HQF90898.1"
                     /translation="MTPVSEKPQPWFVFHRDDIVLRHTDPPELPTPEDREILAPLLRN
                     ECPAGAFPGEDLPWGDLDPRSSLPPGFAPFSRRSLWDLYGEGLFVRAGRAFHLAYWER
                     THAFCGCCGAPTQRHPDEVALRCPRCEHVTYPVISPAVIMAIRRDDKILLARGAHFVS
                     GTYSVLAGFVTPGETLEQAVAREVKEETGIDIDEITYFASQPWPFPQSLMVAFKARWA
                     GGEIAVDRRELEDARWFSPGEDLPIMPSSRSVSRRLIDDWRRSTGATPD"
     gene            complement(13016..13687)
                     /locus_tag="PL162_02325"
     CDS             complement(13016..13687)
                     /locus_tag="PL162_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HQF90899.1"
                     /translation="MALFHEIGEDYRAVRRHDPSIPPGMRGVGEILFCTPGLWALLAH
                     RGIHFLYAGLRLPVLPRFLSILMRWITGIEIHPGARVGRGVFIDHGMGVVIGQTAVVE
                     DDVTLFQGVTLGATGNERGAKRHPTVRRGALIGCGARVLGDIVVGEGARVGAGAIVVE
                     DVPPGATVVGFPAFITRQGGEVRRSARGMVERLERLEDEGRWLRERVEELEGRLLPSD
                     RSEVA"
     gene            complement(14044..15411)
                     /locus_tag="PL162_02330"
     CDS             complement(14044..15411)
                     /locus_tag="PL162_02330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQF90900.1"
                     /translation="MYDVLVLGGGPGGYRAAELAGHAGLKVALVEKYRLGGVCLHRGC
                     IPTKAYYCDVLGKMNPVERMWEKKNRIVEELEGGLRSLMDMHHVEVVEGTGKIHTAQG
                     ETKRLEVTAPDGTLRTLEGRFLILAPGSRSLLPDFPGNTLEEVISGDWAVTRSDLWNP
                     AENDAVRSVAVAGAGVIAVELAGMLREMGRDVTLLKHSGEILRKCDGDIKKKVAQLFR
                     KRGIAMIDSFHIDAVEKVASPQGILRISGSSKNREREEIFCDRLLVAYGMKPILDGYG
                     LENSGVAVEKGAIAVNEAMETNIPGVYAVGDVTGGQMLAHEAEYEALAAVGAMRGKPC
                     PVNRDAMPGCIFTEPEIATVGLTEEEARGRGLSVAVGKAFFLGNGMARAMGKTDGFVK
                     VVADADQDLQILGVHMVGPEAASLIAEATLAVGARMTVRQVAYTVHPHPTTSECFKDA
                     LFRLL"
     gene            complement(15651..17135)
                     /gene="gcvPB"
                     /locus_tag="PL162_02335"
     CDS             complement(15651..17135)
                     /gene="gcvPB"
                     /locus_tag="PL162_02335"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HQF90901.1"
                     /translation="MKRCVKPIFEKSQAGRQGVALPALDVPETPWEKLLPSEFVRKTP
                     AGLPEVSEVDVVRHFTALSQLNFGVDEGFYPLGSCTMKYNPKINENAARMPGFAAIHP
                     FQGEENVQGALELLFHLQEMLAEITGMRAVTLQPAAGAHGEFTGILLMRAYHDARGES
                     HRRTMIVPDTAHGTNPASAAVAGFDVVQVPSNARGNVDLEALRGLLDHRVAGIMLTNP
                     NTLGLFEEHIAEVAELVHGAGGLLYYDGANANAILGKVRPGDMGFDVLHLNLHKTFST
                     PHGGGGPGSGPVGVGERLVPFLPKPVVIRKDQRYVLDFDRPQSIGRVRSFFGNFGVLV
                     RAYAYILSMGPDGLREVAENAVLNANYLMALLQGRYDVKFSGPCKHEFVLSAHRQKEA
                     HGVTALDLAKRLIDFGIHPPTIYFPLLVEEALMVEPTETEGKETLDAFAEAMLAIADE
                     AEQDPEIFAEAPHSTLRSRFDETLAARNPVLRWTAETSGESCGK"
     gene            complement(17132..18481)
                     /gene="gcvPA"
                     /locus_tag="PL162_02340"
     CDS             complement(17132..18481)
                     /gene="gcvPA"
                     /locus_tag="PL162_02340"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HQF90902.1"
                     /translation="MSRYIPNTEEQRRSMLETIGARSVEELFRDIPPELRFAGDLRIP
                     RALSEPELLAHLRDLAGRNATAESHVCFLGAGVYDHFVPLVIDHLVSRQEFTTSYTPY
                     QPEISQGTLQAIFEYQTMVCELTGMDISNASMYDGASALAEAAMIACAAAKREEVLVA
                     RSVHPHAREVLATYAAARGISVREVPFGPDGRVNLQELEALLSPRTGAVVVQNPNFFG
                     VLETLEALGDRTHDAKALFVVSTDLLPLALLEPPGAFGADVVVGDGQPLGQPMSFGGP
                     AFGFFATTKALMRKMPGRVVGQTVDRRGSKGYVLTLQAREQHIRREKATSNICSNQNL
                     CILRATIHLSLLGPRGLRDVAASCFQKAAYARDRLVTSGAFTPLFDAPFFREFALKTP
                     EPVEHLNARLLKDGILGGYDLGRSYHELAGGWLVAVTEKRSREEIETLAEKAGVRVA"
     gene            complement(18495..18890)
                     /gene="gcvH"
                     /locus_tag="PL162_02345"
     CDS             complement(18495..18890)
                     /gene="gcvH"
                     /locus_tag="PL162_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HQF90903.1"
                     /translation="MATKVEQGYKYTATHEWVRVEGSRAFVGVTDFAQHAMGNIVYVE
                     LPDVGKALQAGEDFCVVESVKGAHDIFAPISGTVVEVNGDLEDAPQKVNEDPYGSWIA
                     VIDMSHPDEVKDLMDAAAYATHCENAPAH"
     gene            complement(19000..20100)
                     /gene="gcvT"
                     /locus_tag="PL162_02350"
     CDS             complement(19000..20100)
                     /gene="gcvT"
                     /locus_tag="PL162_02350"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HQF90904.1"
                     /translation="MKKTPMYDEHKSLGGNMVEFCGWNLPVHYEVGIVEEHRRVRSAA
                     GLFDVSHMGEVWISGEGAEAWLQRLVTNDVEAMHDGQVVYNFMCYPNGGVVDDLLVYR
                     FSRTRYYLVINAANVEKDVAWLKMNLPPSGVELRDVSSQTSEVALQGPAAERILQKLV
                     SFDASALGFFHFVPQTTLDGVDVLISRTGYTGEDGFEIYAPWEAGAQIWRAVMAAGKD
                     EGLLPVGLGCRDSLRFEACLPLYGQEISPSITPLEAGLGFFVKLDKKELIGGAALRGL
                     KEGGLSRKSVALELLDKGVPRHDQEVRYEGRPVGFVTTGGYAPSLDKSIALAMVPADL
                     AAEGTEIAVVIRDKEKRARVIKKPFYRKNYKK"
     gene            complement(20144..21142)
                     /locus_tag="PL162_02355"
     CDS             complement(20144..21142)
                     /locus_tag="PL162_02355"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HQF90905.1"
                     /translation="MEKGRLWWAESWSVDPWENLAMEECLWRRTGPGEVGLFLWQNRH
                     TVVIGRHQNPWKECRVDALERDGGRLARRLSGGGAVYHDLGNLNFTFFLGKGLYDFGR
                     QLEVIARAVRSLGIPAEVSGRNDLLAEGRKFSGNAYFRGKHADFHHGTLLVDVDLGCL
                     ERYLHVSPGKLEGKGVASVRSRVVNLKEMNSRVDGIHCRRALREAFEEEYGELGAPLS
                     LEDVVPRDELVEVRGRYGGDLWRFGRTPPFSAAFERRFSWGQVELRFDVVSGVVRSCA
                     IFSDALDGELMARIPGALERAPFDPGALVGALTEAFGLEGPEEIEDMKNWLASAPT"
     assembly_gap    21162..21261
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            21285..21743
                     /locus_tag="PL162_02360"
     CDS             21285..21743
                     /locus_tag="PL162_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQF90906.1"
                     /translation="MSIKILVAVDLSKFSEALVQYGHELAHRLNVQVDFIHVLPHASL
                     FRGYEPWLPPEVSVEVREIAQKKISYWLRKAEEANPRPSEHQHRIIVEEGVPSDTVIS
                     QAKAGGYNLIVVGHQGHSALEYLFVGSTTTSIARHAHCSVLIFRPGLEAL"
     gene            21755..>22626
                     /locus_tag="PL162_02365"
     CDS             21755..>22626
                     /locus_tag="PL162_02365"
                     /inference="COORDINATES: protein motif:HMM:NF017346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-utilizing N-terminal
                     domain-containing protein"
                     /protein_id="HQF90907.1"
                     /translation="MEDGSLPLRGIALAPGVALARAVVWRRIPFEEAKGEGQGPDRER
                     GRLAQARNEAREELAALRRHAAQTVGEERAAVFDAHVLMLDDPLFVGGMEEEIARGLS
                     APDAVVRKTAEIRQLFEGLPDPYLRERGADVEDVGRRLFRHLLGVPSLELSAVADPPV
                     LVAEELSPSETAGLSANSVAALVTERGGATGHTAILAKSLGIPAVGGIEGLLDSVAPG
                     DWVGVDGDRGEVVLRPSPSELILWEKRRELNRQELQVLRGLRDLPAISADGVSVELWG
                     NISRPEETEEVVAQG"
     assembly_gap    22627..22726
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <22727..23254
                     /locus_tag="PL162_02370"
     CDS             <22727..23254
                     /locus_tag="PL162_02370"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HQF90908.1"
                     /translation="IFLPQLRALLRAGAFGDLRIMFPMVAGPDDLGRARDALERARKS
                     LERDGVPFGPVKVGAMIEIPSAALQARELASLVDFFSVGTNDLTQYALAADRMNPRVA
                     PWYDPFSPGVFRLLEMTAEAARERGIELGMCGEMAGDLRALPRLLALGFTELSMTPAQ
                     IPRVKRALRGLRLRG"
     gene            complement(23307..23576)
                     /locus_tag="PL162_02375"
     CDS             complement(23307..23576)
                     /locus_tag="PL162_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HQF90909.1"
                     /translation="MEERRVVVRNDHGLHARPAALFVQKAGSFACRVQLAKEGGTAVD
                     AKSILSVMGLGVENGETIVLVADGEGEKDALDALEALLATPSTQG"
     gene            complement(23671..24111)
                     /locus_tag="PL162_02380"
     CDS             complement(23671..24111)
                     /locus_tag="PL162_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HQF90910.1"
                     /translation="MGSGGGGVSAPWWSGGLRFACQGCGRCCRGEPGAIWFTPEEERR
                     LASALGMELGAFRRHHVTARWGAPSLRERPGGDCVLLDPETGRCRSYVARPLQCRAYP
                     FWPSVVVSPESWREHARRCPGMDQGRLWPGEVIARIISRFPPRF"
     gene            complement(24117..24479)
                     /locus_tag="PL162_02385"
     CDS             complement(24117..24479)
                     /locus_tag="PL162_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90911.1"
                     /translation="MTTFLSSSLGAEDLFGPSRSAESGKAPKSPERQREELKEACQHF
                     EGILLSMLWKDMMRTAREMGDGSRRPFAPLEDTAVEMASQSLSEGGGVGLWKVLYDQL
                     QESLPAEEGKGAPRELRG"
     gene            complement(24476..>24631)
                     /locus_tag="PL162_02390"
     CDS             complement(24476..>24631)
                     /locus_tag="PL162_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HQF90912.1"
                     /translation="QPKQMVSLPASSSVQDLVDALNAVGATPRDIITILQGISQAGAL
                     HGELVIE"
     assembly_gap    24632..24731
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<24732..25293)
                     /locus_tag="PL162_02395"
     CDS             complement(<24732..25293)
                     /locus_tag="PL162_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HQF90913.1"
                     /translation="MAKRIGLMGVILALGIAMVSGAGGEIHPEVRLKDLADVEGVRSN
                     QLVGLGVVVGLAGTGDKGTMVNQMMQNMAQQFGVAVDSKAIKSKNAAVVTVTCQLPPF
                     VRPGQTVDVSVSTMGDAKSLQGGVLLQTPLKGANGEVYVVAQGPLLVGGYAASGKGAQ
                     VSKNATTVGRIPGGGIVEREVAMEFAP"
     gene            complement(25309..25881)
                     /locus_tag="PL162_02400"
     CDS             complement(25309..25881)
                     /locus_tag="PL162_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HQF90914.1"
                     /translation="MGRIGIMAVAMVFLALTGASGESLWREGASLFSDARPTAVGDIV
                     TVVVSERTSVKDEAKTDLTKTNSSNLEDGVGLLGKILSALGFRSDSSMKGEGSTERKH
                     TVSTRITCLVTEVLPNGNLVLEGGRDLQVHGETLHLLFRGVIRPRDVAHDNTVESDMV
                     ANAEIGIKGKGSLTRVQRPGLLTQLFQAIF"
     gene            complement(25886..26785)
                     /gene="flgA"
                     /locus_tag="PL162_02405"
     CDS             complement(25886..26785)
                     /gene="flgA"
                     /locus_tag="PL162_02405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HQF90915.1"
                     /translation="MGSERGRAWSRLLVVFWMGCLAGASFGVAEGASLKVEIPSPLAV
                     RRGTLYLGEVGRLEGAAELVSRASKARLPFREGGWTQEDLWRSLGESGLVGETVELRM
                     PSRVELLPEGEATAEVRRLSRWPWGLEVHAEGSPCGALVSPGDLPPGVRATVLRFRGS
                     GGRECVVPVRIRWLVPAAVTLRALEGGAVIRPEDYRVALREWEGSASPPDPLSWPGQI
                     LVRPLPEEAVLMVGDVRRVPVVRRGAVVRLVYVVGGLRVEAWGETLQRGALGETIRVR
                     NTVSRAVVSGVLVTPETVWVERK"
     gene            complement(26789..27577)
                     /gene="flgG"
                     /locus_tag="PL162_02410"
     CDS             complement(26789..27577)
                     /gene="flgG"
                     /locus_tag="PL162_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HQF90916.1"
                     /translation="MMRSLWAGASGMIAQQTNLDVVSNNLANVNTAGFKKIRADFEDL
                     LYQIDREPGAPVEPDSMIPDGIQVGLGARVVGTSRMMAPGNLKVTSNQLDVAIEGDGF
                     FQVTLPNGDIAYTRDGGWRMDGNGQIVTSNGYLLEPAITVPEDAVAVAISPTGDVSVL
                     MPGDPAPQNVGQLQLARFVNPGGLKAVGKNLFLETAASGAPQIANPGEAGMGTTIQGI
                     LELSNVQVVEEMVNMIVAQRAYEANSKTIQTADDLLRIANSLRR"
     gene            complement(27603..28394)
                     /locus_tag="PL162_02415"
     CDS             complement(27603..28394)
                     /locus_tag="PL162_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HQF90917.1"
                     /translation="MFRGLYAGASAMLVQERMLDVTGNNLANVNSTGFRGRTPVNKAF
                     PTLLMEKIDGQPGETFPPKPEWLRRRDPIQDAVFANVLSETAMSIQAGAVRVTDNPLD
                     VALEGPGFFVVADGAGNTFYTRAGHFVRDNEGRLATHEGQLVQGQGGTITLGEGWTAH
                     IDDGGQVVVDGEVVDRLQVVTFDNPTYLRQAGRSLLQETAGSGAPQEEANPTLARGAL
                     EMSNVNVVSEMVRMVEANRAYEAAAKAVSVQDESASRLTQTYGRV"
     gene            complement(28447..29487)
                     /locus_tag="PL162_02420"
     CDS             complement(28447..29487)
                     /locus_tag="PL162_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HQF90918.1"
                     /translation="MFGNDIGIDLGTATVLIYIRGKGVVLREPSVVALDKDNNRILAV
                     GEDAKRMVGKVPGHVVAIRPLRDGVIADYTMTEAMLRHFIKKVTRGTGRFFRHRVMVC
                     VPSGATDVERRAVLEAALEVGARDAYLIEEPMAAAIGAGINVGEPRGNMVVDVGGGTS
                     DIAVISLGNIVISESLRVGGDKFDEAISRYVRRNYNLAIGEQTSEDMKVRIGNCYSRG
                     EVMTMNIRGRDLVLGLPREIQITSAGVAEAISEQVQSIVDGIRRVLELTPPELSADII
                     EGGVVLTGGGALLRNLPELVTEQTGISCHVADQPMECVALGTGKAMEELDKLKDSGTI
                     LSTSRKGGRRPR"
     gene            complement(29608..30390)
                     /gene="truA"
                     /locus_tag="PL162_02425"
     CDS             complement(29608..30390)
                     /gene="truA"
                     /locus_tag="PL162_02425"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HQF90919.1"
                     /translation="MKYAVRLAYDGAAFSGWQAQGHGLGVQQVVEEALGALHGVLVPI
                     SAAGRTDAGVHARGQVVSFEVPRSWEPEMLRRALDARMPQSVRALGVSPVSPDFDARH
                     SARWREYVYFLWAAPATYPHLHTMTWWIHQPWDWEMVRQACVLLPGTHDFSAFCRSAD
                     RPERTVRTIFSARLARRGSLWWFRVRGNAFLTNMVRILVGSLAEVGRGRRPVAWFASL
                     LEGHSRAMAGMTAPPQGLFLWNVGYGDEGEKLLWEKGESPIV"
     gene            complement(30387..31205)
                     /locus_tag="PL162_02430"
     CDS             complement(30387..31205)
                     /locus_tag="PL162_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HQF90920.1"
                     /translation="MKFLDHMTLGQYIPVDSLVHRLDPRCKILGTVALMGALFVGDGA
                     GAMGAWGGLFFLLASASKIPVSMALRGVRPVVILIAFTAVINLFFSSGEPLWSWGILR
                     VSREGMVAAFTMSFRLFFLVLFAGFLTLTTSPMELADGLERLLSILKPIGFPAHEMAM
                     MMTIALRFIPTLLDETDRILKAQLARGADLDRGGIGRRIKAFVPVLVPLFILVFQRAE
                     DLATAMEARCYRGGDGRTRMHPLRWTLRDSGALVGLCLVTVFVCVLDARWPLLR"
     gene            complement(31202..32044)
                     /locus_tag="PL162_02435"
     CDS             complement(31202..32044)
                     /locus_tag="PL162_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQF90921.1"
                     /translation="MSLSVKDLSFTYHAGTPLARRALDSVSFEMNSGQWMAVVGPTGS
                     GKSTLAQHLNALILPQEGKVTVDGMTVTHRSPDLREVRRRVGLVFQYPEQQLFAENVY
                     DEVAFAPRNWGVDEGRLEALVARSLAQVGLETSLSSRSPFSLSGGQKRRVALASVLAA
                     EPEYLVLDEPTAGLDGGGVRDLLSLIAELVRGGMGVLQITHDLELALSRCDAILVLDH
                     GRVLGMGSPQDLVDPLCRAVLPGLIIPPVLDFSRRLRERGLAVPLTMDAEELGRRLEK
                     GGRY"
     gene            complement(32029..32850)
                     /locus_tag="PL162_02440"
     CDS             complement(32029..32850)
                     /locus_tag="PL162_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQF90922.1"
                     /translation="MGSVCELREVGYTYPGAKTPALAGISLRIAPGAWVALLGSNGSG
                     KSTLARIFNALLVPTQGSSIVCGWDTGKEEHHAAVRREVCMVFQNPENQIVAATVEED
                     VAFGPENLGLPSEEIVSRVRWALEVVGLTALGRQPVYALSGGQKQRLAVAGALALRPR
                     CLVLDEATSMLDPQGRADLASVLRELHAEGMTLVSITHRLEEILDCDEIHVLHRGQQV
                     WHGAPLDFFAVPRPEWGVEDPPLWRLWRRLRSGNRIPRTIFPRGKEMAEALCPSR"
     gene            complement(32862..33215)
                     /gene="rplQ"
                     /locus_tag="PL162_02445"
     CDS             complement(32862..33215)
                     /gene="rplQ"
                     /locus_tag="PL162_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HQF90923.1"
                     /translation="MRHRVDHSTLGRYGSHRHAMLANMAASLFLHGAISTTLVRAREA
                     RRVAEKLITRARGGTLQDRRLVIARMPHKEAVCKLFDEIAPRYQGRPGGYTRIVKTGN
                     RVGDASPMVVLELVE"
     assembly_gap    33246..33345
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<33346..33764)
                     /locus_tag="PL162_02450"
     CDS             complement(<33346..33764)
                     /locus_tag="PL162_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HQF90924.1"
                     /translation="MENLQTEIRVEESSRTPQYARIVVEPLERGYGVTLGNSLRRVLL
                     ASIDGAAITAVRIDGVLHEFSTVPGVREDVIELLLNLKHVPVRSHSDEIRILRIDLEG
                     PKDVKAADVQRDSDVDFVDPEAPICTLEVGARLAAEL"
     gene            complement(33789..34418)
                     /gene="rpsD"
                     /locus_tag="PL162_02455"
     CDS             complement(33789..34418)
                     /gene="rpsD"
                     /locus_tag="PL162_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HQF90925.1"
                     /translation="MSRYTGAVCRQCRAEGVKLFLKGDRCYTGKCAISKNNVRPGQHG
                     AKRVKTSEYGLRLREKQKLRRFYGMGEAQFRRFFGIAVKMAGQTGHNFLQTLERRLDN
                     VIYRMGLGVSRSQARQIVCHGHVSVNGRKVDVASFLVKAGDVILVREGSRDLVLLKEN
                     AEVAGSRAIPAWMTFNADRMEGSVVALPTREQIDSPVNEQLVVEFYAAK"
     gene            complement(34449..34841)
                     /gene="rpsK"
                     /locus_tag="PL162_02460"
     CDS             complement(34449..34841)
                     /gene="rpsK"
                     /locus_tag="PL162_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004641037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HQF90926.1"
                     /translation="MAKRVQRRGKRKEKKHVSYGVAHIHSTFNNTIISITDKQGNLLS
                     WSSGGNVGFKGTRKSTPFAAQMAAQAAAKVAQDCGLAELDVVVKGPGPGRESAIRSLQ
                     AAGLQVNMIKDETPIPHNGCRPPKRRRV"
     gene            complement(34872..35249)
                     /gene="rpsM"
                     /locus_tag="PL162_02465"
     CDS             complement(34872..35249)
                     /gene="rpsM"
                     /locus_tag="PL162_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HQF90927.1"
                     /translation="MARIAGVDLPRDKRVEIALTYIFGIGLPTSQKILRVTGVSPDTR
                     VKDLTEEEAQRLRAEIENSHMVEGDLRREVAMNIKRLIEIGCYRGLRHKQGLPVRGQR
                     TKTNARTRKGPRRTVAGKKKATK"
     gene            complement(35256..35381)
                     /gene="rpmJ"
                     /locus_tag="PL162_02470"
     CDS             complement(35256..35381)
                     /gene="rpmJ"
                     /locus_tag="PL162_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HQF90928.1"
                     /translation="MKVRPAVKTICEHCRVIRRHGVVRVICSKNPRHKQRQGARR"
     gene            complement(35456..35674)
                     /gene="infA"
                     /locus_tag="PL162_02475"
     CDS             complement(35456..35674)
                     /gene="infA"
                     /locus_tag="PL162_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HQF90929.1"
                     /translation="MAKDDVIEVRGKVSEPLPNAMFRVELENGYKVLAHVSGKMRMHF
                     IRILPGDRVLVQLSPYDLTRGRIVYRYK"
     gene            complement(35726..36496)
                     /gene="map"
                     /locus_tag="PL162_02480"
     CDS             complement(35726..36496)
                     /gene="map"
                     /locus_tag="PL162_02480"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HQF90930.1"
                     /translation="MISLKNEKDLQVMRHAGAVVADVLRLMRDLVQPGITTMELDRRA
                     DELIGRSGGTAAFKGYVMTGISRPFPGSICVSINEEVVHGIPGVRRLEEGDIVSVDVG
                     VLLQGYYGDAACTYTVGKTSAQRQHLLEVTLASLHAALAVVRPGRTLGDVGHAVESRV
                     LQAGLGLVRTYAGHGIGKKLHEAPQVPNYGRPRAGITLKKGMTIAIEPMVMTGREEVV
                     SKPDAWTVVTADGSDAAHFEHTVAVLEDGVEVLTPWES"
     gene            complement(36493..>37077)
                     /locus_tag="PL162_02485"
     CDS             complement(36493..>37077)
                     /locus_tag="PL162_02485"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HQF90931.1"
                     /translation="STGDILRSHVARGTPLGLQAKSYMDAGKLVPDGVMIDLIKNRLG
                     DEDCRQGFILDGFPRTVAQAEALDLALGAQGQDIDKVILFQVTDEVVVKRLCSRRVCK
                     QCGAIYHVTFHPTKQEGICDACGGAVIQRDDDREEVIRHRLQVYHDQATPLVAYYRAK
                     DILAEVDGGVRDAVLRVLASQDLQGTRGSSERSQ"
     assembly_gap    37078..37177
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<37178..38157)
                     /gene="secY"
                     /locus_tag="PL162_02490"
     CDS             complement(<37178..38157)
                     /gene="secY"
                     /locus_tag="PL162_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HQF90932.1"
                     /translation="MIDSFRDAFRLPDLKRRILFTLGVLLAFRLGAHIPTPGIDPEAM
                     ARMFQQGGALDFLDLFAGGALRRFSVFALGVAPYINSSIVMQLLVVVVPALEKLQKHE
                     GEDGRKKIVMYTRWGTVLFALVQAVGMTFWLGNMGIFSGNILDGVVVALTVTAGSVAV
                     MWLGEEVTDHGIGNGISMLIFAGIVARIPEAIVQTWTLLRMGEMSALAMLVGLVLMVG
                     VVAGCVLLQEGQRRLPVQYAKRVVGNKVYGGQSTFIPLKVNQSGVIPIIFASSVLLFP
                     YTILNLFSGEWAQMLKGLFSPGSPVYLVLYVILIVFFAYFYTAVVFNPVD"
     gene            complement(38158..38604)
                     /gene="rplO"
                     /locus_tag="PL162_02495"
     CDS             complement(38158..38604)
                     /gene="rplO"
                     /locus_tag="PL162_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HQF90933.1"
                     /translation="MELHELRPAKGSHHVAKRKGQGLGSGNGKTAGKGNKGHKSRSGG
                     GVRPGFEGGQMPLSRRIPKRGFNNFRFAKNYQVVNLVDLEERFEAGAEVDVEALRRAL
                     LVRHDQLPVKILAQGELTKGMTVRAHAFSAEALRKIEAAGGKAEVI"
     gene            complement(38622..38807)
                     /gene="rpmD"
                     /locus_tag="PL162_02500"
     CDS             complement(38622..38807)
                     /gene="rpmD"
                     /locus_tag="PL162_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HQF90934.1"
                     /translation="MVKLRITWKRSAIGHPERQGRTIRALGLRRLHYTVVHEDSPQVR
                     GMIRAVSHLVEWSEVPE"
     gene            complement(38821..39324)
                     /gene="rpsE"
                     /locus_tag="PL162_02505"
     CDS             complement(38821..39324)
                     /gene="rpsE"
                     /locus_tag="PL162_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HQF90935.1"
                     /translation="MANRTISAKGVELSERVVSINRVSKVVKGGKRFKFSILVVVGDG
                     TRFVGVGIGKAREISEAVRKAIEKAKKNLVDLKKVGHTIPHPVIGDFGAARVLLKPAA
                     PGTGVIAGAVVRAIMELGGVKDVLTKVIGRTSNSINVAYATMEAVKIMRTPDEIRRLR
                     GLDRKEA"
     gene            complement(39338..39709)
                     /gene="rplR"
                     /locus_tag="PL162_02510"
     CDS             complement(39338..39709)
                     /gene="rplR"
                     /locus_tag="PL162_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HQF90936.1"
                     /translation="MIEKRSRNEMRQVRHERLRRTLSGTPDRPRLSVFRSLKHVVAQI
                     IDDEAGRTLVAASSVEPDIRATLGGRTGNAETARTVGRLVAERALAKGIKAVVFDRGG
                     HMFHGRVKALADAAREAGLDF"
     gene            complement(39727..40266)
                     /gene="rplF"
                     /locus_tag="PL162_02515"
     CDS             complement(39727..40266)
                     /gene="rplF"
                     /locus_tag="PL162_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HQF90937.1"
                     /translation="MSRIGRKAISIPKGVTVNVDGARVVVKGSKGELSMDILPQVGVQ
                     VAGDVATVSRVDDLKTSRALHGMTRAMISNMVHGVSSGFERVLEIVGVGYRAQMKGKT
                     LVLNLGYSHPIEFDPPTGIEIATDGPTKIFVRGIDKQAVGQVAANIREFRAPEPYKGK
                     GIRYAGEVIIRKAGKTGSK"
     gene            complement(40280..40684)
                     /gene="rpsH"
                     /locus_tag="PL162_02520"
     CDS             complement(40280..40684)
                     /gene="rpsH"
                     /locus_tag="PL162_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HQF90938.1"
                     /translation="MYVNDPIADMLTRIRNANLAYHEMVDLPVSKIKVGIAKILKEEG
                     YVRNFKVINDPKKPFPVLRLFLHYGPSRERVMQGIKRISKPGRRIYMGVGELPKVMGG
                     LGIAVVSTSRGLLTDADARKQGLGGEVLCFVW"
     gene            complement(40713..40898)
                     /locus_tag="PL162_02525"
     CDS             complement(40713..40898)
                     /locus_tag="PL162_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HQF90939.1"
                     /translation="MSRVAMEYKAQQEPKFKVRKHNRCPLCGRPKAFMRDFNMCRCCF
                     RKLAREGKIPGVVKSSW"
     gene            complement(40915..41457)
                     /gene="rplE"
                     /locus_tag="PL162_02530"
     CDS             complement(40915..41457)
                     /gene="rplE"
                     /locus_tag="PL162_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HQF90940.1"
                     /translation="MIPRIRQKYREEIVPRLSSKFGYKNVMQVPRVEKVMVNIGVNEA
                     KQDIKYMNISMEELGIITGQRPLMRRAKKSVAGFKVREGMPVACFVTLRGNKMWEFLD
                     RLVSLTLPRIKDFQGVSRKSFDGRGNYNLGLREQLIFPEIDYDKVIRVRGMNITITTT
                     ARTDEEGAALLSEIGFPFMR"
     gene            complement(41475..41801)
                     /gene="rplX"
                     /locus_tag="PL162_02535"
     CDS             complement(41475..41801)
                     /gene="rplX"
                     /locus_tag="PL162_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HQF90941.1"
                     /translation="MGKMRLKKGDRVRVISGKYAGVEGKILRRDVDRDMIVVEGVNMA
                     TKHVRPSKKNPKSGIVKQETPFYACKAMLVCPACGKATRVGHAYLDNGKKVRLCRQCG
                     EVVDKV"
     gene            complement(41815..42183)
                     /gene="rplN"
                     /locus_tag="PL162_02540"
     CDS             complement(41815..42183)
                     /gene="rplN"
                     /locus_tag="PL162_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HQF90942.1"
                     /translation="MIQLRTVLNVADNTGAKRVTCIQVVGGSFRKVGTVGDVIVASVR
                     EAIPNSNTEKGKVVKCVIVRTKKEVRRRDGSYVRFDDNAAVIIDGNGDPKGTRIFGPV
                     ARELREKKYMRIVSLAPEVV"
     gene            complement(42187..42477)
                     /gene="rpsQ"
                     /locus_tag="PL162_02545"
     CDS             complement(42187..42477)
                     /gene="rpsQ"
                     /locus_tag="PL162_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HQF90943.1"
                     /translation="MEENVSRARSRDGIVVSTKMQKTVVVRVDRMAKHPLYGKPVIHA
                     KKYQVHDEENTCRMGDRVRIVETRPLSRHKRWTLAEVLERAPILTGSDGGEA"
     gene            complement(42484..42699)
                     /gene="rpmC"
                     /locus_tag="PL162_02550"
     CDS             complement(42484..42699)
                     /gene="rpmC"
                     /locus_tag="PL162_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HQF90944.1"
                     /translation="MDTKDMRDLTLDELKGKHRQYKEELFNLRFQHAIGQLGNTGRIR
                     DVKRTIARILTMVREKELGLDRAPARR"
     gene            complement(42699..43124)
                     /gene="rplP"
                     /locus_tag="PL162_02555"
     CDS             complement(42699..43124)
                     /gene="rplP"
                     /locus_tag="PL162_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HQF90945.1"
                     /translation="MLSPKRVKYRKPFRSPLKGMAKGGTTVAFGDYGLQTLEGVWLTA
                     RQIEAARVAISRKMKKGGKVWIRVFPDVAYTKKPLETRMGKGKGNPEWWVAPVKRGKI
                     MFEISGVPRDVAEEAFRTASHKLPVKVRLVAREGMDGEE"
     gene            complement(43131..43856)
                     /gene="rpsC"
                     /locus_tag="PL162_02560"
     CDS             complement(43131..43856)
                     /gene="rpsC"
                     /locus_tag="PL162_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HQF90946.1"
                     /translation="MGQKVHPVGYRVGVIRDWESRWFASGKTYVKHLHEDLKLRDWIK
                     KRWFHAGVSKVEIERIGNVLRFTVWTARPGVVIGKGGQEIQIVRDELQRISGSRIMIN
                     VQEIKHPDMDAQVVSEGVASSLERRISFRRAMKQAIFRAMKSGARGIKIQCSGRLGGA
                     EIARTEWYLEGRLPLSTLRAEIDYGFGEAHTIYGVIGIKVWIYRGDAARKLRKGAVGA
                     DQPQAPVAAQPVPNRDRERDKRR"
     gene            complement(43872..44216)
                     /gene="rplV"
                     /locus_tag="PL162_02565"
     CDS             complement(43872..44216)
                     /gene="rplV"
                     /locus_tag="PL162_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HQF90947.1"
                     /translation="MDKIREAHAMAKQVRISPNKARQVLRLIAGKNANDAVMTLRYTP
                     KKAARFIEKILNSAVANADHNFGMDVDKLVVVKAVADTGTTLRRFRPVSMGRAHPFRH
                     YTCHITVAVAER"
     gene            complement(44229..44510)
                     /gene="rpsS"
                     /locus_tag="PL162_02570"
     CDS             complement(44229..44510)
                     /gene="rpsS"
                     /locus_tag="PL162_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HQF90948.1"
                     /translation="MARSLKKGPFVDAKLVKRIEDMNHSGKKRVLKTWSRRSTVLPEM
                     VGHTIAVHNGKTHMPVFVSENMVGHKLGEFAPTRRFGGHAGQERTSRVK"
     gene            complement(44529..45353)
                     /gene="rplB"
                     /locus_tag="PL162_02575"
     CDS             complement(44529..45353)
                     /gene="rplB"
                     /locus_tag="PL162_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HQF90949.1"
                     /translation="MALKKFKPTTDGRRFMTISAFEEITKGKPEKKLVESLKNFAGRN
                     NTGRITMRHRGGGAKRKYRIIDFRRNKIGVPGTVAAFEYDPNRTARIALIHYLDGDKR
                     YILAPQGLSVGDTVMAGPKADIRPGNALKLADIPLGTVVHNIELQPGRGGVMVRSAGA
                     GAQLMAKEGKYAFIRMPSSELRLILLECMATIGLVGNEDHENTTIGKAGKSRWLRRRP
                     HVRGMVMNPCDHPLGGGEGKTKGNKHPVSPWGTPAKGYRTRKRKQSDKHIVRRREK"
     gene            complement(45376..45675)
                     /gene="rplW"
                     /locus_tag="PL162_02580"
     CDS             complement(45376..45675)
                     /gene="rplW"
                     /locus_tag="PL162_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HQF90950.1"
                     /translation="MIGRSDHDVIVRPIVTEKSNALMEHGTYTFEVLPDANKIEIRNA
                     VEKVFKVKVSKVHTINVHPKPKRMGAFLGKSRSWKKAMVTLVEGQRIEFFEGANA"
     gene            complement(45672..46298)
                     /gene="rplD"
                     /locus_tag="PL162_02585"
     CDS             complement(45672..46298)
                     /gene="rplD"
                     /locus_tag="PL162_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HQF90951.1"
                     /translation="MPNVKIVDFEGTSLGERELLDAVFAAPVHMAAMHQVVVAQLANA
                     RQGTHNAKDRSDVRGSNRKLFRQKHTGRARRGSLRSPVMVGGGVAHGPHPRDYSQKVN
                     KKLRALAMRSVLSLKVRGDRMMLLENPVLEAPKTKAVVAFLSKVGAEKKPLLVVHETN
                     VNLYRSAANVPGAKVLHVDSLNVYDVLNHAHLIMTPEAARKVEEVFSR"
     gene            complement(46314..46943)
                     /gene="rplC"
                     /locus_tag="PL162_02590"
     CDS             complement(46314..46943)
                     /gene="rplC"
                     /locus_tag="PL162_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HQF90952.1"
                     /translation="MSKGIIGRKLGMTQVYDENGRAVPVTVVEAGPCPVVAIRTPEKN
                     GYVAIQLGFGAIKKGRVSKPLQGHYDKVGVEPRRWLREFRLRDPKAYELGQYVTVEIF
                     TPGEKVNATGRSKGKGFAGVMKRHNYGGGAKSHGASVIHRRPGSIGCHTYPGRVFKGK
                     TMAGHMGDERVTVKNLTVFAVDAENHLLLIKGSIPGARNGLVMVHKHEA"
     gene            complement(46983..47288)
                     /gene="rpsJ"
                     /locus_tag="PL162_02595"
     CDS             complement(46983..47288)
                     /gene="rpsJ"
                     /locus_tag="PL162_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HQF90953.1"
                     /translation="MSKKIRIKLKAFDHRVLDTSALQISETAVRSGAKVAGPIPLPTE
                     IKKYTILKSPHKDKDAREQFEVRTHKRLIDILDPTQRTMDALMQLNLPSGVDIQIKL"
BASE COUNT         8429 a        16007 c        13823 g         8607 t
ORIGIN      
        1 gccagctccg ccacggtgac aaaagacact tctcccaaga acttcagcgt cacgtggagc
       61 tgatcaccag cgacccacct gatcctgggg gccacgcggc gcagctcgcc gatccagctt
      121 tccacccgcc gggcaaggtc gtgttgtagc ggcagacaga tgaaactccg catcgtgtcg
      181 atcacggccc tcctccttct ccttccgccc cagatcccag cgcctccacg aggcgctcca
      241 gggcaaaatg cacgctctga gacctcacct cgtctcggtt ccctgaaaat aggcggtgcc
      301 aggcccgctc gctttgggcc tcggccagag cgaaccatac ggtaccccgg ggcttttccg
      361 gccccccccc atcgggcccc gcgatgcccg tgaccgccag ggccaggtcc acggcgagga
      421 cccgccgggc ccccgacgcc atggcccggg cacaggcctc gctcaccgcc ccctggcgat
      481 cgatgacctc ttcggggact cccaggaggg agttttttat cccgttacta tagctcacca
      541 cggcccccat aaaaaggcta gaagcgccgg ggatggcggt gatggccccc gaaaggaggc
      601 ctccggtgca agactctgcc agtcccaggg tctttttttg ccgggtgccg agagccacta
      661 gacgttcggc cagggcccaa aggcggtcgc ggtccaaaat ctccccccct attccaaaag
      721 ggtcgccatg cccccggcca ggaaaaacca ggagaaaaag cgcagaatgg cgttggtggc
      781 gatccccgcc aagaggtcgt ccgccatgat gcccacacct ccgggaaggg cttcggcctg
      841 gcggatggga aagggcttga cgatgtccag aatgcgaaag acgaggaaag agcccagcgc
      901 cagggaaggc tccaggccga actgggcaac ccacatgccc gccacctcgt cgatgacgac
      961 ctcggaggga tcggtctccc ccttccgccg ggcgtagcga tccgccgccc aggtgcctaa
     1021 aagggtcacg gcgacgatga gcagggcgaa taggggtaag tggcgcgccg gatagatcga
     1081 ggcccccagg aaggccaccc ccgaccccac cgtgccggga acggaggaaa agcaccccac
     1141 cccccccgcc gtggccaccc acccttccca gctccgagga aggggtctgc gaggggcgat
     1201 cacgacgcca cctcccccac gaggtcgtgg gggtgagctt caaggacgcg caccgacacc
     1261 acctgtcccg gcgtcaccgg gatggagcca ctccgaattt ccacttctcc atccacctcc
     1321 ggggcctccc ggaaggaacg cccccaggcg aggccttcat cgggatcggc agattctacg
     1381 aggacggcca aggtgcggcc gacccaccgc tcctgacggg ccagggaaag gcgctcctgc
     1441 aacgccatga tcctttcctg gcggcggtcg gcggtgcgac gggacaccga gggtaaaagg
     1501 gccgccgccg cagtgccctc ctcgggggaa aagacgaagc tgcccaggcg atcgaaggaa
     1561 atttcttcca ggaagtctcg cagggtctcg aaggcccgac ggtcctcgcc gggaaagccc
     1621 accatgacgg tggtgcgcag ggcaaaatcg ggagaggctc ttcgggcggc gaggaagagc
     1681 ctccggagct cttcggtacg ggcggggcgg ttcatggctt cgaggatccg ctcgtcggcg
     1741 tgctggatgg ggacatcggc gtagggcaag agctggggca gggaaagcat gtgctcccag
     1801 aagctttctt tcaggtgcga cggatggagg taaagcaggc gcacccacac gtcccggggg
     1861 agctctcggg agaggtcttt taggagggat tccagtcccg cctccccccg atcttcgccg
     1921 tagaccgcca gatcctgggc cacaagacag atctcccggg cccccgacgc caccaggcgc
     1981 tccgcatccc gaaggatttc tttgggagat gcgctctgca ggggtccccg gagggcgggg
     2041 atcaggcaat aggaacagcg atggtcgcaa ccttcggcaa tcttcaggta gcgggaccag
     2101 gggagaccct cgggcaaggg atctcgcttc ccctcttcct gggcccccag ggtccgggcc
     2161 agggcatctc cctcccccgc tccccaccag gaatccaccc aggggagctc ccggcgcaac
     2221 tcatcgccgt ggcggtttac cagacacccc atgacggcca cgcgccccag gcgcccttcc
     2281 tgcttgaggc gctccacgtc caggaggaga tccacattct ctctcaccgc cgaggcgatg
     2341 aagccgcagg tgttgaccac gaggaggtcg gagggctccg aagggggcac caggaggtgc
     2401 cccccccctc gcagctggga ggcaaggcgc tccgaatcca cctggttctt ggcgcatcct
     2461 aaagagatta tcgacacctt catcgtgggg gcttacttca cttccatggt gccgtcaggg
     2521 aagaactcga cggtggccac tcctccgccg gtacccttac ccatggcccg ggtctgcccc
     2581 ccccaggtgg cggtgatggc cgatgatttt cccatgcgca cccgcacggg ggacgtgagg
     2641 gggatgcggc gctcctctcc ggccttcatg gtggtctcga agaccgtcct tcccccctga
     2701 acggcccgca cccaacaagg agacgaggcc aggaggatca gatttttccc ttcggcatcc
     2761 tggctcttgg cgatctgatt catctccttg agccacgggt agtcctcgct cgtcgcatcg
     2821 gcggaacgat ggggctcggg ggaaaggatt tccggcgaaa ggacccgatc tccgatcgaa
     2881 gcgtccttag aagctccgga ggtcccgaca tcgctggagc ccccgggaag cgccgcccgc
     2941 tgcgccggag tgggggcccc ggtgggcgaa aggaggggca ggggcgtcaa ggcccggtcg
     3001 acggaggcgg catcggggcg ccccccctgg ccgatctggg cccgtagaac gtcccactcc
     3061 ttccagacaa ccccggcggt gaggatcgcc aggccgacga ccatgagata gacccacctt
     3121 cgggaaatcc ggcggaaacc cgaagacaga ggggaacaag ccacggcgct gtccgtatcg
     3181 aggtcatcgg gctgctctag gggcacctcc tcggcgaagg ccttccacag ctgccccgcc
     3241 ccgagaaact ccaggtagtt gcggatgaaa ccccgggcat agatcggcgc cgggaagcat
     3301 ccctcgtccc ccttttcgat ggcgtcgacg aagcgctccc taatatggag ggcctctgcc
     3361 acatcttttt gagaaaggcc ccgggcctcc cgaaggcgga ccgcttcctc tcccaatttt
     3421 ttcagagaaa ggccttcttc gtgcatactc tagcctcctg ggccaccctg tgcagggttt
     3481 cggccgcttt tcctagatct gaagcgccga aaagggcgct ccctgccacc aacacatcgc
     3541 accccgccgc cgctgcggca cccacggtgc tctctcccat gcccccgtcg atctggatca
     3601 cgaaatctaa cccccggatc tctcgctccc ggacgagggc gtggaccttg ggcaacacct
     3661 cggggagaaa actctgcccg ccgaagcccg gattcaccga catcaccagc accagatcca
     3721 ccagatgaag ggtgggaagg atcatctcca cgggggtgcc ggggttaaga gagacccccg
     3781 gagacactcc ggcctggcga agggcctgca aaacccggtg caggtggggg gtggcctcgc
     3841 cgtgcaccgt gaccacagag ggtccttccg ccagaaaggc gtcaatgaat cgctccggag
     3901 gttccaccat gagatggaca tccaaaacgc cgccggagaa atcccgggcc agggcccgca
     3961 acagggcggg accataggaa aggttgggga caaagtgacc gtccatgata tccaagtgga
     4021 gccaacggat ccaaggatcc cccagtcgct ccagatcggc accgatcctc aaggggtccg
     4081 ccgccagaag agagggagag atgaggggcc ccgactcccg caggacgcgg gcgcttactg
     4141 gtaacgatcc tgccatacga attctcctcc cagatagatg gtcaccacgg cctcctgggc
     4201 ataagtctcc tccaacttga tatattctcc cccctgaacc gttttgtcca ggaccgatct
     4261 cgtgccattt cggtccacca cctcgatgcg caggttcatg ggctttacca gcgggggcac
     4321 ctgatagcgg attttggcct ttttaccccg agatccccct tccggggcag gggtggcctt
     4381 tttagggggc tcttctccct tgggaggggc ggccaccacg gtggggccca cctgaggggg
     4441 aaccccctcc tctccttccg cccggggcgg tacccctacc acaggcactc gcaccgtccc
     4501 cggaggggaa ccaacggagt ctcccccggt ccctccctct ccttgagggg taggagtctt
     4561 ccctcccgcc gcccgatcgg cggtggccac caccagctcg acccggcttc ccttggagac
     4621 ctttgtcccc ggcgacgggg acatgcgcac caccagtccc gggggagtcg ccaaggtgta
     4681 ctgataccgg acacctccca ccctaagacc cccgtcctcc agcattttcc gggcctcttt
     4741 ttcggggcgc cccgccagtt ctggcacctt tactagcccc tggggggccc ctccgccgta
     4801 gctcaccaga aggtccaccc tcatgcctgc gggaacggcg gcgggagagg cggggctttg
     4861 ggccaccacg gccccggcgg gaaggttttt atccgccacc cgcaccacgt cccccggggt
     4921 gaatccctcg gcctggaggg tctgcaccgc ctgttcgtac tccatgcccc gcacatcggg
     4981 aagggaaacc tgacgccctc ccttgctcac cttgaggatc accgccttgc ccttgcgcac
     5041 caaggtcccc tccgccgggc tctgggccag gacggtgccg ggttttttaa gggagtccaa
     5101 ctgatccacc cgggccacca gctccgattt ttttagtatt tccaccgcat caatgacgga
     5161 aaggccctcc accttgggca tcgagacgtc gttgcctccc aaaaatacgg cgtaaaaggt
     5221 gaagaccccc gaccccacga ttaccagcaa caccaaaagc agcgttatgg gcaccagttt
     5281 ccgcatgcca tgctccccct gtaaggttcg tctctctggc cccgccgctg cggcgccgtc
     5341 tctacaaaaa aatccacgaa aatgagttcc ctaggccgac accttcatca gcacggtcac
     5401 gtaaaagcca tccacccagg gcaatgcggg gaagatcgaa aaaccccagg ggcgccctcg
     5461 acgccaggga gcctttcccg tcaggaccgg gacttcgacc acggagggat cccgggccag
     5521 cacgtcggcc accaccgctt cgttttcctc acgaaagagg ctacaggtgg cgtacagcac
     5581 gtaccccccc gacgccgcca acgacgcccc tcgggccagg agccggcgct gcaaagccgc
     5641 cagagccccc acctcggagg gggtgacgcg ccacttggcc tccggatggc gcccccaggt
     5701 gccgctcccc gagcaggggg catctaccaa caccagacga tagcgcctcg aagcctccaa
     5761 ggcaagggcg tctccttcga tgacggcccc caccaggtgg cgccgggcca tctcccctcg
     5821 aaaggcccgg caacgccccg gggatttttc ccaggcctcc accgcccccg ccggaaggcg
     5881 cgagatcagg tgggccgtct tacccccccg tccagcacac atgtcaagca ccgggccatc
     5941 ccccgccagg gagagggcca gctcactcac ccgcatcgaa gactcggtct ggggcgaaag
     6001 ggccccctgg agaaaaccgg gaacggaggg gatgtggggc gtggcggcgg tgcggaggac
     6061 tccggaaagg gccggagagg gccaggccct aaggcccgct tccccaagcc tctcggccca
     6121 tccctggggg gactctcccg gggaaaggca aaaggaggtg tagggcctaa tggaagccat
     6181 cttcacaagc tggcgggccc ccgacgggcc aaagtcctct tcgaagcggc gagccgccca
     6241 atcggggact ccgcggaaga gggcctggtc caaaagggcc ccgctcgccc ccagacgctc
     6301 catcagggag atcccccggg gctcgatgcg tcgcaggacg gcgttgacca gggccccctc
     6361 tcgggggctg atgcgccgca cgtattccac cagggcgttt cccagggccg ccggagcaaa
     6421 ggtccgcaag gtacaaaggc ccgccgcccc caccacgagg gccctcatgg ccccaggaga
     6481 tgggtccctc cggggagggg tcagggcctc gcggaccaga cgctcccaca gggccttccg
     6541 acggaagacc gcataggcca gcgtggccgc cagggagagg tcttcgggag tgccgatctt
     6601 ctccgaagct aggcgccgca acgcttcact caggaactcc cccctatcca ccccttcgac
     6661 cagccgcagg gcggcctcca cgcccctcat caggacacca cttcggggta gacgtctttc
     6721 cccgagatcc gagtcaccac caaaagctgt tcggggatca ccacgccccc aaggccgtat
     6781 ctccgccgtc cctggagctc cactcccaat atttccaact gagacacgtc cacatcggtg
     6841 gtgatccaac gctgggccca cacccccagc gagggcccca cgagctgcgc cgcccctagg
     6901 gccaccaaca ccgaaaaaag ggacatcctc ccggtgatca tcagcagaag aagaaaggcc
     6961 accgccgcca gcaggttggc caccacagcg gtggtgccac accgggggtg tatcgccaac
     7021 cccgcctcgc ccccttggag cctccgcagg gcggtacggg ccgcccccaa caccgtggcc
     7081 ccatcgaaga ggtcgccccg tacggtaaag cccttttcga aggccagccc cgaaaggtta
     7141 agtgggccgt aagtttcttc caaaacgtgg agggtggcgt gctccagggc gtgattgcgc
     7201 cgcacccggg ggttgacgag gatcccatag agctgggaag gccccgacgc gatccaaaag
     7261 agggactggg ccgcaaaacc cagggggacg aaggccagaa agaacaccgc cgccgccccc
     7321 aggacgagga agatgatccc cgggaggaaa agccccaggg ccaaaaaagc caccaagagg
     7381 ggcgcacacc cttgcatcgt ctcacactcc tcgctggcgg catccgaggg ccaaagtcat
     7441 tcgtcgcgcc cccgcaaaag cagcaaacgc aggagctgta ccgccgccat gacggtggcg
     7501 gccacgtagg taagggcggc ggcgttcaag acccgacggg ctccagcaat ctctgcggtg
     7561 cccaacacct gggtctctcc caggaggcgc agggcccggg agctggcgtc aaattccacg
     7621 ggcaaagtca cgaggtgaaa gaccaagatc ccggcgaaaa ggaggatgcc cagatccatg
     7681 agagcaggga tccgcatgat gatccccaga aagaacaggg gcatggccag ggtggatccc
     7741 aggttcgtca cggggacgat ggcattcctt atcctcaaag gggcgtatcc ctgaaggtcc
     7801 tgcaccgcat gccccgcctc gtgagccgcc acgccgatgg cggcaatgct ggtgctgccg
     7861 tagacgtcgt cggagaggcg caataccttg ctacgaggat cgtaatggtc agtgagggat
     7921 ccgggaacac gctccaccgc cacgtggttc aggccgaaac ggtctagcag gcgccgggcc
     7981 gcctccgagg cccgcacccc ccgcgccgaa gacacctggc tgtatcggcg gaaggtggaa
     8041 gagatccgca tctgggccca tagggccaag atgaccgccg gaatgagcag caacaacgtg
     8101 gcatccatgg gaaaaaacat cgcctcagcc tcactttctc tacgaaaaaa tccctaattt
     8161 cccctatccc gggagctttt ctcaggccta aaggataacg actcccgccc cctacaccgc
     8221 acgatcgacc tctggggaca aaacccctgg caccgaagcg gcgaggctcc cccggggata
     8281 tccgggcgcg ataaggggcc gcagaccagt gccccaggag cgtaccgacg gacccggcct
     8341 tccccccata aacacgagaa taccgatacg aagagaactc cgcatcggta ttctacacga
     8401 agggccccgg gagggccact ggaaaatctg gcgcccttta gtcgtaaaac cgccggatgg
     8461 tatccaccac ccactcctgt tcttcggggg tgatctcggg gaagatcggc agggccaagc
     8521 tctcccggct taggcgctcg gcctcggggt agtccccttc ctgccctccc aggaaacgga
     8581 agcagggctg gaggtggagc cccagagggt agtagaccct agaggtgatg ccctcggcgc
     8641 cgaggaaggc ctggagatcg tcccggcggg agcagcgaat cacgtactgg tggtagatgt
     8701 ggtagttctg ggggtcctcc gccgggggcg tcagggcgtc caaaaggccc cgctcctcga
     8761 agaggatacg gtatctctct gcggcgaggc ggcgctcttc gttccactcc tccaggtgcc
     8821 tcaggcgtac ccggaggatg gcggcctgaa gggtgtccag gcggctgttg agccccactt
     8881 cttggtggaa gtaggcttct tccgccccgt ggacccgaag gcggcgcagg cgtgtcgcca
     8941 gggcatcgtc ggtggaggaa atcatgccac catcgccata gcatcccagg ttttttgtgg
     9001 gaaagaagga aaaacacccc aggctcccca cgctccccga ccgggccagg cgcccgtcga
     9061 tccgacgcca ggccccaaag gactgggcgc agtcttccac cagacggagg cgcccctccc
     9121 gcaagacggg atcgaggagc tccacctcgg ccatctggcc aaaaagatgc accgggagga
     9181 tggctcgagt tcggggagaa atcttctcca gaacctgatc catgcgaagg ttgtaggtct
     9241 cggggtcgat atccacgaag atcggccggc cccccagccg ggtgatactg ctcacggtgg
     9301 caaaaaagct ataaggggtg gtgatcacct cgtccccctc ccggaggtcc aaggccatga
     9361 gggccaagag cagagcgtcg gaccccgagg cgcacccgat ggcgtgaggc acctccagat
     9421 aggattggac ttctttttca aaagccccca cggcggggcc caggatgaag tgttggctct
     9481 ccaacacctc gtccatcgct tcggcgatct cctcgcggat acgtcggtag ttgcgcttca
     9541 agtccagagt gggaagggat cgtgtggcca tcatggggcc tccttgtcat cttggcatgg
     9601 aatgggtcta aaggggcttt ccccctacga ctcgttatcc ccggggcgcc ggagattgtg
     9661 gaccgcagaa cgccccatcc ctcgggtttt ttagcgatga atcagtgatc cggctgagcc
     9721 atgcccgtca tggtggtcct caggtgatcc cgcatgagga gctccgcccg gaccccgtcg
     9781 cggtcccgta gggcccggag aaggtcttta tgctccgcca tgctgggatt gacctcgtct
     9841 tcatagaagg ggtcgtagaa gacgatgtat acgttggtcc gggcgaggat gttttccacg
     9901 taggaggcca ggatgcggtt tcccgtggcc tccgccacgg tgcggtggaa tgcttcgttg
     9961 atttccatgt agagctccag gtttcggtct cgaaaggcct cctcttccgc cccgatgatc
    10021 tcttccatac gccgaaagtg cttttccgac atggcccggg ccgccaggcg ggccgccaga
    10081 cattcgagtt gctcccgcac cacgtaggcg ctctccatct cccgcctgga gggggacgcc
    10141 agccgggctc cactgttggg gacaatgtcc accaacccct cgctctccag ccgccgcaag
    10201 gcttctcgta cgggagtacg acttaccccc atctgcacgg cgatgttgac ctcgggaagg
    10261 cgctgtcccg ccttgatttt cttggtgatg atccggtgcc gcagctctcg gtatacataa
    10321 tctgcggagg tcgtatataa ttgggtctct agcatggggg gcctcctccc ttgcgctggt
    10381 caagtgggac ttttgcgcgg agcctaaagg tctccgggga gcattttatc acttggccaa
    10441 agcatgcacc atgttttttt gattcctctc cgacggggac agaaaaaata gggccaggtc
    10501 tcacgccccg ctctcgagga atccaccatc ggcctcgtcg cccctgcgcg gagcgcaggc
    10561 ccggcgctcc gatgcagatc acgaccctat ctggccccac cgccccgtcc ggagcgtgga
    10621 acgaagcgtc taaggaggtt ttgccatgcc attgcccacc gccgccctgc cccgggaaga
    10681 tgtcaccctc cttccctctc ccctcatcga gttcattcgc ctgggcctgg aacactccgc
    10741 catccccctt acctcgggac agcctcatcc gtcgtgctac cccctgacgg agctggcgac
    10801 agcggcctcc gaggcgatcc gaggcgaggg agagcgccac ctccggtacg gcgccaaccg
    10861 gggcctgggg aagcttcgcg ccttggtgaa caaagagtta atcaatcgat ccctctttcc
    10921 ccccgacctc tctccggatt ccctggtact caccgtgggg gcccaggggg ccttcgacct
    10981 gctatgccag gcggtgctgc gcccgggaga ggcggtggcc ctggatgccc cctgttaccc
    11041 cgacacatgg tgcacggtgg tccgacgagg aggaaaggtg attcccatcc ccgtagatgc
    11101 cgaggggatg gacgtggacg ccctggaagg ggccctgcac caaggaatcc ggccccgcct
    11161 ggtctacacc atccccacct accagaaccc cacggggtgc tccctctctc gggagcggag
    11221 ctcccggctg gtggccctgg cggagcgctt cgactttctg gtcgtgctgg acgatcccta
    11281 ccgtttgctc cccctggacg agcccctgcg ctccggggag gagttcttcc cctggaaggg
    11341 gtgggactcg gggcgcgtgg tggccctggg ctctttttcc aagatcctgg ccccggggtt
    11401 gcgcgtggga tgggctcatg ccgctccgga gctggcagac gccctcgcca cgctccagga
    11461 gatgagccac atttcgctcc ccgccctaga tgccctggcg gtggcgtcgt ttttggagca
    11521 ccacggcctg gaggggcaga tccaaagggt ccggaccttt ttgcagggcc agcgggccgc
    11581 ccttatcgac gccatggcgg aagcccggct ggaggagctg gggtgtggca ccatggccag
    11641 cgcggggggg tgttttctag ggctcgtgct tcccgctgga cgagaggcct cccccgtggc
    11701 ccgaaccctg gcggcggatt tcggcgtggc caccgtgccg gagcgggcct tctggccccc
    11761 ccacggggaa gggaccccgg accgattcct gcgcctgtcg ttttcctggg ccactccgga
    11821 ggaatttcgc cgctccgccc aggcattccg ccaggtcctg acgacgccat gaggcttttg
    11881 gtgctagtcc tatccctggg gtgcgtggtg gcctacggac gggccttggg tgagctgggg
    11941 gggtacttcc tcggcaaggg aagcctggtg gtggtagggg tagggcttct tggggggact
    12001 ttggcagggg tgggggccgt ggggctctgg cgccgctacc tggtgccccg ggatccctcc
    12061 gccccaaaag gccccccgaa ggtcgacccg cgcctctaga ttcttgtgga aatcaactta
    12121 aaggctcgcg ccccctcatc cggggcgcgt cgagcccttc cgtcatcgcc caaggatgcc
    12181 cttgggcccg cgaaggaggt accatgaccc ctgtatcaga aaagccccaa ccctggttcg
    12241 tcttccaccg agacgacatc gtcttgcgcc acaccgatcc cccggagctc ccgacgcctg
    12301 aggaccggga aatcctggct cccctgctcc ggaacgagtg tcccgcaggg gcctttcccg
    12361 gcgaggacct tccctgggga gatctcgacc cccggagctc cttgcctccg ggctttgccc
    12421 cgttctcccg gagatccctc tgggatctct atggagaggg ccttttcgtc cgggcgggcc
    12481 gggccttcca cctggcctac tgggagcgta cccacgcctt ttgcggctgc tgtggcgccc
    12541 ccacccaacg gcaccccgac gaggtcgccc tccggtgccc ccggtgtgaa cacgtgacgt
    12601 acccggtgat ctccccggcg gtgatcatgg ccatccgccg ggacgacaag atcctcctcg
    12661 cccggggggc ccacttcgtc tcgggaacct atagcgtcct cgcgggtttc gtaactcccg
    12721 gagagactct ggagcaggcc gtggcccgag aggtgaaaga ggaaacgggc atcgacatcg
    12781 acgagatcac ctacttcgcc agtcaaccct ggccctttcc ccaatccctc atggtggcct
    12841 tcaaggcccg gtgggccggg ggagagatcg ccgtggatcg ccgggagctc gaagacgccc
    12901 gatggttctc cccaggggag gaccttccca taatgccctc ttcccgcagc gtctcccgga
    12961 ggctcatcga cgactggcgc cgcagcaccg gggcgacgcc ggactgagaa gaccttcagg
    13021 ccacctcgct ccggtcggag ggaaggagtc gcccctctag ctcctccacc cgttctcgga
    13081 gccaccgccc ctcgtcttcg aggcgctcca atcgctccac catgcctcgg gcgctccgcc
    13141 gtacctcccc gccctggcgg gtgatgaagg cgggaaaacc taccactgtg gcgcccgggg
    13201 ggacgtcttc caccacgatg gccccggcgc ccacccgggc gccctctccc accacaatgt
    13261 cccccagcac ccgggcaccg cagccgatga gggccccgcg ccgcacggtg gggtggcgtt
    13321 ttgcccccct ttcgttgccc gtggccccca gggtgactcc ctggaagagg gtgacgtcgt
    13381 cttccaccac ggcggtctgg ccgatgacca cccccatgcc gtggtcgatg aaaacgcccc
    13441 gacccacccg agctccggga tggatctcta tgccggtgat ccatcgcatc aatatcgaaa
    13501 gaaatcgagg gagcaccgga aggcggagcc cggcgtaaag aaaatggatt ccccggtgcg
    13561 ccagaagggc ccaaagtccc ggggtgcaaa agaggatctc ccccaccccc cgcatccccg
    13621 gcggaatgga ggggtcgtgg cgccgcaccg cccggtagtc ttctccgatc tcgtgaaaga
    13681 gggccatatc tttactcctt tctccggccg gtcagggacc ggaaagggca taaaagaggg
    13741 gaccgtcggc gctgcgacgg tcccctcttt tataggctcc ctattaccga aaggggtaga
    13801 gatcccccct ctttcgtgcc cgtgccgccg cagcatcgcc ctatggccgc tgcggcaaat
    13861 cccgcagggg gaactatgcg tctcccaccc gacaacaaca gcaccacacg aaagaaagct
    13921 tcaccgcaac ctcccctttc ctgttgggag cgatcctacc acgagaaggg aattttgtct
    13981 agaactcctt agaatttttt tggcccccgg cgggacaata acccggggga tcgatctctc
    14041 ccgtcacagg agacgaaaga gggcatcctt aaagcattcc gaggtggtgg ggtggggatg
    14101 caccgtatac gccacctggc gcacggtcat gcgggccccc acggcgaggg tggcttcggc
    14161 gatgagggac gccgcctcgg gacccaccat gtggaccccc aggatttgga ggtcctggtc
    14221 tgcatccgcc accactttca cgaaaccgtc cgtcttgccc atggcccggg ccatgccgtt
    14281 gcccaggaaa aaggccttgc ccacggccac cgagagtccc cgtccccggg cttcttcctc
    14341 cgtgagtccc acggtggcga tttccggctc ggtgaagatg caccccggca tggcgtcccg
    14401 gttgacgggg cagggtttcc cccgcatggc ccccacggcg gcgagggcct cgtactccgc
    14461 ttcgtgggcg agcatctgcc ctccggtgac atctcccacg gcgtagaccc cggggatgtt
    14521 ggtttccatg gcctcattca ccgcgatggc tcccttttcc acagccacgc cggagttctc
    14581 cagaccgtag ccgtcgagga tgggcttcat gccgtaggcc accagcaggc ggtcacaaaa
    14641 gatttcctct cgctcccggt tcttggagct cccggaaatc cgcaggatcc cctggggaga
    14701 agcgaccttt tccacggcgt cgatgtgaaa gctgtcgatc atggcgatgc cccgcttgcg
    14761 gaagagctgg gccacttttt tcttgatgtc tccgtcgcat ttccgaagga tctcgccgga
    14821 gtgtttgagc agggtgacat cccgccccat ttcccggagc attcccgcga gctccaccgc
    14881 gatgaccccc gcccccgcca cggccacgga gcgcaccgca tcattttccg cagggttcca
    14941 gagatcgctc cgggtcacgg cccagtctcc ggagatgact tcttccaggg tgtttccggg
    15001 aaagtcgggg aggagggatc gggaacccgg cgccaggatc agaaatctcc cctccagggt
    15061 gcggagggtg ccgtccggag cggtgacctc caggcgtttc gtctcgccct gggcggtgtg
    15121 gattttcccc gtgccttcca ccacctccac gtggtgcatg tccatgaggg agcgcaaacc
    15181 cccttccagc tcctccacga tgcggttttt cttttcccac atgcgctcta cggggttcat
    15241 ttttccgagg acgtcgcagt agtacgcctt ggtggggatg cacccccggt ggaggcacac
    15301 gccccccagg cggtactttt ccaccagagc aaccttgagt cccgcatgcc ccgcaagctc
    15361 cgccgcccga tatcctccgg ggcctccccc caacaccagt acgtcgtaca tggctcttgc
    15421 cgtcctttca ccacgcttcc cccctaaaac gcgatccccg cccctccccg atccgccccc
    15481 tcgaggggtg gaccggaaat ttgccagggc ggggaacgcg gggggttcct tccgggcggc
    15541 ggcgccgcca aaccgccatg tttcccagct tatgaaatat cgctatttgt ttagatcggc
    15601 ggcccctttc tccgtcggca cgacccgggg ggcgtcaccg gatctttccg tcacttgccg
    15661 caggattctc ccgaggtctc cgccgtccag cgcagcaccg gattccgcgc cgccagggtt
    15721 tcgtcgaagc gggaacggag ggtgctgtgg ggagcctcgg cgaagatttc tgggtcttgc
    15781 tccgcctcgt cggcgatggc gagcatggct tcggcaaagg catccagggt ctctttgcct
    15841 tcggtttcgg tgggttccac catgagggcc tcttccacca ggagggggaa gtagatggtg
    15901 gggggatgga tgccgaaatc gatgaggcgt ttggccaggt ccagggcagt gacgccgtgg
    15961 gcttcctttt ggcggtgcgc ggagaggaca aactcgtgct tgcagggccc ggagaacttc
    16021 acgtcgtacc gaccttgcag aagggccatg agatagttgg cgttgagcac ggcgttttcc
    16081 gccacctccc ggagaccgtc ggggcccatg gagaggatgt aggcataggc ccgcaccagc
    16141 actccgaagt tgccgaagaa ggagcgcacc cggccgatgg actggggacg gtcgaaatcc
    16201 agcacgtacc gctggtcctt ccggatcacc acgggcttgg gaaggaaggg caccaggcgc
    16261 tctcccaccc ccacgggtcc cgatccgggg cctcctccgc cgtggggggt gctgaaggtc
    16321 ttgtggaggt tgaggtgtag cacgtcaaac cccatgtctc cggggcggac cttgcccaaa
    16381 atggcgttgg cgttggctcc atcgtagtag agcaatcccc cggcgccgtg caccagctcc
    16441 gccacttcgg cgatgtgttc ttcaaagagc cccagggtgt tggggttcgt gagcatgatc
    16501 cccgccaccc ggtgatccag gagcccccgc agggcctcca gatcgacgtt tccccgggcg
    16561 ttggagggaa cctgcaccac gtcgaacccc gccacggcgg cggaggcggg attggtgccg
    16621 tgggcggtgt cgggaacgat catggtgcgc cggtgggact cccctcgggc atcgtggtac
    16681 gcccgcatga ggaggatccc cgtaaactct ccgtgggctc ccgcagcggg ttgcagggtc
    16741 accgcccgca tgccggtgat ctcggcgagc atttcctgaa ggtgaaacag gagctccagg
    16801 gccccctgca cgttttcctc cccctggaag gggtgaatgg cggcaaagcc gggcatccgg
    16861 gcggcgttct cgttgatttt cgggttgtac ttcatggtgc aggaccccag aggataaaag
    16921 ccttcgtcca caccgaagtt gagctgggac agggcggtga agtggcgcac cacatccacc
    16981 tcggaaactt cggggaggcc cgccggggtc tttcggacaa attccgaagg aaggagcttt
    17041 tcccaggggg tttcggggac gtccagggcg ggcaacgcca ccccctgacg tccggcctgg
    17101 ctcttttcaa agatgggttt cacgcagcgc ttcatgcgac tctcaccccc gccttctccg
    17161 ccaaggtctc gatctcctcg cgggaacgtt tttccgtcac cgccacaagc catcctccgg
    17221 cgagctcgtg ataggaccgg cccaggtcgt agccccccag gatcccgtcc ttcagaagcc
    17281 gggcgttgag atgttccacg ggctccgggg tcttcagggc aaattcccgg aagaaggggg
    17341 cgtcaaaaag gggcgtaaag gccccggagg tcacgaggcg atcccgggcg taagcggcct
    17401 tctggaagca cgatgccgcc acgtcccgca atcctcgggg gcccaggagg ctcaggtgga
    17461 tcgtggcccg caggatgcag aggttctggt tggagcagat gttcgacgtg gctttttcgc
    17521 gccggatgtg ctgctcccgg gcctgcaggg tgaggacgta ccccttgctc ccccggcgat
    17581 ccacggtctg tcccaccacc cggccgggca tcttgcgcat gagggccttg gtggtggcaa
    17641 aaaatccgaa ggcgggtcct ccgaagctca tgggctggcc caggggctgc ccgtccccca
    17701 ccaccacgtc ggcgccaaag gccccggggg gctccagcaa cgccaggggc aaaagatcgg
    17761 tggagaccac gaagagggct ttcgcgtcgt gggttcggtc gccgagggcc tccaacgttt
    17821 ccagaacgcc gaagaagttg gggttttgga ccaccaccgc cccggtacgg ggcgaaagca
    17881 gggcttcgag ttcctgaaga ttcacccgac cgtcggggcc gaagggcacc tccctcaccg
    17941 agataccccg ggcggcggcg taggtggcca gcacctcccg ggcatggggg tgcacggaac
    18001 gggccaccaa cacctcctcc cgcttggcgg cggcgcaggc gatcatggcc gcctccgcca
    18061 gggcggaggc cccgtcatac atggaggcgt tggagatgtc catgccggtg agctcacaga
    18121 ccatggtctg gtactcgaag atcgcctgga gggtgccctg gctgatctcg ggctgatagg
    18181 gggtgtagct cgtggtgaac tcctgccggg acaccaggtg gtcaatgacc aggggaacga
    18241 agtggtcgta gaccccggcg ccgagaaagc acacgtggct ttccgccgtg gcgttgcgcc
    18301 cggcgaggtc tcgcagatgg gccaggagtt cgggctccga gagggctcgg gggatacgaa
    18361 ggtctccggc aaagcgcagc tccggaggga tgtcccgaaa gagctcctcc acggagcggg
    18421 cgccaatggt ctccagcatg gagcggcgct gttcctccgt gttgggaatg tagcgcgaca
    18481 tgatcccgtc tctcctagtg ggcgggggcg ttttcgcagt gggtcgcgta ggcggcggcg
    18541 tccatgaggt ctttcacttc gtcggggtgg gacatgtcga tcaccgcgat ccagcttccg
    18601 tagggatcct cgttgacctt ctggggagcg tcttccagat cgccgttgac ttccaccacc
    18661 gtccccgaga tgggggcgaa gatgtcgtgg gcccccttga cggattccac cacgcagaaa
    18721 tcctctcccg cctgcagggc tttccccacg tccggcagtt ccacatagac gatgtttccc
    18781 atggcgtgct gggcaaaatc cgtaacgccc acaaaggccc tggatccctc gactcgcacc
    18841 cactcgtggg tggcggtgta cttgtaaccc tgctccactt tcgtcgccat gacattattc
    18901 ctccctttcg gcccctcggc ctctagatat ctctttccgg tctcccgcga aaagcgacgc
    18961 gacgccccat tccgggtccc atggagggcc cgatcggggc tatttcttgt agtttttgcg
    19021 gtagaagggc tttttgatca cccgggcccg cttttccttg tcccggatga ccacggcgat
    19081 ctccgtcccc tcggcggcca gatcggcggg gaccatcgcc agggcgatgc tcttgtccaa
    19141 ggagggggca tacccccccg tggtgacgaa gcccacaggg cgtccttcgt accgcacctc
    19201 ctggtcgtgg cgcggcacgc ccttgtccag aagctccagc gccacgctct ttcgggaaag
    19261 gcccccttcc ttgagccctc gcagggcggc gccgccgatg agctcttttt tgtcgagctt
    19321 cacgaaaaag cccaatcccg cctccagggg ggtgatactg ggggagatct cctggccgta
    19381 gagcggcagg cacgcctcga agcgcaggct gtcccggcac cccaacccca cgggcaggag
    19441 gccctcgtcc ttccccgccg ccatgaccgc ccgccagatc tgggcccccg cctcccaggg
    19501 ggcgtagatc tcaaagccat cctcccccgt gtaacccgtg cgggagatca gcacatccac
    19561 gccgtccagc gtggtctggg ggacgaagtg aaaaaatccc agggcggagg cgtcgaagga
    19621 gacgagcttc tgcaggatgc gttccgccgc tgggccctgg agggccacct cggaggtctg
    19681 cgaggacacg tcccggagct ccaccccgga ggggggaagg ttcatcttga gccaagccac
    19741 gtccttttcc acgttggcgg cgttaatgac caggtagtac cgagtgcggg agaaacggta
    19801 gaccaggagg tcgtccacca cgcccccgtt ggggtagcac atgaagttgt agaccacctg
    19861 gccgtcgtgc atggcctcca cgtcgttggt caccaggcgc tgaagccagg cttcggcccc
    19921 ttcgccggaa atccagacct cgcccatgtg ggagacgtcg aagagccctg cggcgctccg
    19981 cacccgacgg tgttcctcca cgatgcccac ctcgtagtgc accggcagat tccacccgca
    20041 gaactccacc atgttgccgc ccaaactctt gtgctcatcg tacatcggcg tttttttcat
    20101 cctgtcacct cccaaaagtt tgaccgacgg cgatccgccg gggtcatgtc ggggcgctcg
    20161 caagccaatt cttcatgtct tctatctcct ccggcccctc gagaccaaag gcctcggtca
    20221 gtgccccgac cagggctcca gggtcgaagg gggcgcgctc cagggccccg ggaatccggg
    20281 ccatcaattc tccgtccagg gcatcggaaa agatggcgca ggagcgcacc acccccgaga
    20341 ccacgtcaaa gcgcaactcc acctgtcccc aggagaagcg gcgctcgaag gccgcagaaa
    20401 agggaggggt gcggccaaag cgccaaaggt ctcccccata gcgcccccgg acttccacga
    20461 gctcgtcccg gggcaccacg tcctccaggg aaaggggggc ccccagctcg ccgtactcct
    20521 cttcgaaggc ctccctcagg gccctccgac aatggatccc gtccacccga gagttcatct
    20581 ctttcagatt gaccactcgg gatcgcacgg aggccactcc cttgccttcg agctttcccg
    20641 gagacacatg gaggtagcgc tccaggcatc ccaagtccac gtccaccaga agggtcccgt
    20701 gatgaaagtc ggcgtgctta ccccgaaagt aggcgttgcc ggagaacttc ctcccctccg
    20761 ccaacaggtc gttccggccg ctgacttccg ccgggattcc cagggatcgt acggcccgag
    20821 cgatgacttc caactgccgg ccgaaatcgt agagaccctt gcccaggaaa aaggtgaagt
    20881 tgaggttccc caggtcgtgg tacaccgccc ctcccccgga gagacgccgg gccagccgcc
    20941 ctccgtcgcg ctccagggcg tccactcggc actccttcca ggggttttgg tggcgcccga
    21001 tcaccaccgt gtgacgattt tgccataaaa agagccccac ttcccccggg cccgtccggc
    21061 gccacaggca ctcttccatg gccaggttct cccaggggtc caccgaccag gattcggccc
    21121 accataggcg tcctttctcc actccatccc cccccttcga cnnnnnnnnn nnnnnnnnnn
    21181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21241 nnnnnnnnnn nnnnnnnnnn ntcgggccca aggaggaatg ccccatgtcg atcaaaattc
    21301 ttgtcgccgt agacctgagc aagttttccg aggccctggt gcagtatgga cacgaactgg
    21361 cccatcggct caacgtgcag gtggatttca tccacgtgtt gccccacgcg tcgctcttcc
    21421 ggggctacga gccctggctt ccccccgagg tgagcgtgga ggtgcgagag atcgcccaga
    21481 agaagatcag ctactggctc cgcaaggcgg aggaagctaa cccccgcccc tcggagcacc
    21541 agcatcgcat catcgtggag gagggggttc cctccgacac cgtcatctcc caagccaagg
    21601 cggggggata caacctcatc gtggtgggac accaggggca cagcgccctg gagtaccttt
    21661 tcgtgggcag taccaccacc tccatcgccc ggcatgccca ttgctcggtg ctgattttcc
    21721 gccccgggct cgaagcgctt tagaggccgt cgccatggaa gacggatcct tgcccctgcg
    21781 agggatcgcc ttggcgccgg gggtcgccct ggcacgggcc gtggtctggc gccggatccc
    21841 cttcgaagag gccaaagggg aggggcaggg ccccgaccgg gaacggggcc ggctcgccca
    21901 ggcccgtaac gaggcccggg aggaattggc cgcccttagg cgtcacgcgg cccagaccgt
    21961 gggggaagag agggcggcgg tcttcgatgc ccatgtcctc atgctggatg atcccctttt
    22021 cgtgggggga atggaggagg agattgcccg aggtctttca gccccggacg ccgtggtgcg
    22081 caagaccgca gagatccgcc agctcttcga agggctcccc gatccctacc tgagggagcg
    22141 aggggccgat gtggaggacg tggggcggcg cctttttcgt cacctcctcg gggtcccgtc
    22201 gttggagctc tccgccgtcg ccgatcctcc ggtgctcgtc gcggaggagc tctccccctc
    22261 ggagactgcg ggcctctcgg cgaactccgt ggcggccttg gtgacggaac ggggaggggc
    22321 cacaggacac accgccatcc tggccaaatc cctgggcatc cccgctgtgg gaggcatcga
    22381 agggcttttg gactccgtgg caccggggga ctgggtgggg gtagacgggg accggggaga
    22441 ggtggtcctg cgaccgtcgc cctcggagct aatcctctgg gaaaaacgcc gggagctgaa
    22501 ccgacaggag cttcaggtgc tccgagggct gcgggacctg cccgccatca gcgccgacgg
    22561 ggtctccgtg gagctctggg gaaatatctc ccgccccgag gagaccgagg aggtcgtggc
    22621 ccaaggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatct ttctccccca
    22741 gctccgggcc ctcctccggg cgggggcctt tggcgacttg aggatcatgt tccccatggt
    22801 ggccgggccc gacgacctgg ggcgcgcccg ggacgccctc gaaagggccc gcaaatctct
    22861 agaacgcgac ggggtcccct tcgggccggt caaggtggga gccatgatag agatcccctc
    22921 cgccgccctg caggcccggg agctggcctc cctggtggat tttttttccg tgggaaccaa
    22981 cgacctgacg cagtacgccc tggcggcgga tcgcatgaat ccccgggtgg ccccgtggta
    23041 cgaccccttc tctcccgggg tcttccgcct gctggagatg acggcggagg cggcccggga
    23101 gcgcggcatc gagctgggca tgtgcgggga aatggccgga gacctccggg cccttccccg
    23161 gctcctggcc ctgggtttca ccgagctttc catgaccccg gcccagattc cccgggtaaa
    23221 acgggccctt cgggggcttc gcctccgggg gtgacgccaa aagatccggg ccggggcgat
    23281 ggagccccgg cccgaagtcg ctccccctag ccctgggtcg acggggtcgc cagaagggcc
    23341 tccagggcgt ccagggcgtc tttttccccc tccccgtcgg cgacgaggac gatcgtctcg
    23401 ccattttcta cccccaggcc catgacggag aggatgcttt tggcgtccac cgccgtcccc
    23461 ccctctttcg ccagctggac tcgacaggca aagctccccg ccttctggac gaaaagggcc
    23521 gcaggacggg cgtggagccc gtggtcgttg cggaccacca cccgacgttc ttccatgtca
    23581 catacctccc taaaaaagtt ctttcgtaaa aacgccgccc cgtccccggc tccctcggaa
    23641 atctggggat ggggtccccg tggcgcgcgg ctaaaagcgc ggggggaagc gagaaatgat
    23701 ccgggcaatg acttccccgg gccacaggcg cccctgatcc atgccggggc accttcgggc
    23761 gtgctcccgc cagctttcgg gggaaaccac caccgaaggc cagaaggggt aggcccggca
    23821 ctggagggga cgggcgacgt aactccgaca gcgccccgtc tccgggtcca gcaggacgca
    23881 atcccccccg ggacgctccc ggaggctggg agctccccaa cgggcggtga cgtgatgacg
    23941 gcgaaaggcc ccgagctcca tccccagggc ggaggccagg cgcctttctt cctcgggggt
    24001 aaaccatatg gcgccgggct cccctcggca gcaccgaccg cacccttggc aagcgaaacg
    24061 cagtcccccg ctccaccagg gggcggagac ccctccgccg cccgagccca cggcccctac
    24121 ccccggagct cccgaggagc tccctttcct tcctcggcgg ggaggctctc ctggagttga
    24181 tcgtaaagca ccttccagag ccccaccccc ccgccttcgg agagggactg ggacgccatt
    24241 tccaccgccg tgtcttccag gggagcaaag gggcgccggg agccatcccc catctcccgg
    24301 gccgtacgca tcatgtcctt ccaaagcatg gatagaagga tgccctcgaa gtgctggcag
    24361 gcctccttga gctcctcccg ctgacgttcg ggggacttcg gagctttccc cgactccgcg
    24421 ctccgcgacg ggccgaagag gtcttcggcc cccaggctgg aagaaagaaa ggtcgtcatt
    24481 cgatcaccag ctccccgtgc aaggctcccg cctgggagat gccttgaaga atcgtgatga
    24541 tatcccgagg ggtggccccc acggcgttca gggcgtccac gaggtcctgc acgctggaag
    24601 aggccggaag gctcaccatc tgtttgggct gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24721 nnnnnnnnnn ncgggggcga actccatggc cacctcgcgc tccacgatcc cccctccggg
    24781 gatgcgcccc acggtggtgg cgttcttgga gacctgggct ccctttcccg acgccgcata
    24841 cccccccacc aggagggggc cttgggccac gacgtagacc tctccgttgg cccccttgag
    24901 gggggtctgg agcaacaccc ctccctggag gcttttggca tctcccatgg tgctcacgct
    24961 cacgtccacg gtctgccccg gacggacgaa ggggggaagc tgacaggtga cggtgaccac
    25021 ggcggcgttt ttgctcttga tggctttgga gtccaccgcg acgccgaact gttgggccat
    25081 gttctgcatc atctggttca ccatggtgcc tttgtccccc gtgcccgcca atcccaccac
    25141 cactcccagc cccaccagct ggttactccg cacgccctcc acgtcggcca ggtccttgag
    25201 gcgcacctcg gggtgaatct cccctccagc ccccgaaacc attgcgattc ctagggccag
    25261 gatcaccccc atcagaccga tccttttcgc catgtccctt ccccccggct agaaaatcgc
    25321 ctgaaaaagc tgggtaagga gtcccggccg ctggacccgg gtgagggagc ccttgccctt
    25381 tatgccgatc tcggcgttgg ccaccatgtc gctctccacc gtgttgtcgt gggccacgtc
    25441 ccgggggcgg atgactcccc gaaagagcag atgaagggtc tcgccgtgaa cctgaaggtc
    25501 gcgtcccccc tctaggacca ggttgccatt gggaaggacc tcggtcacga ggcaggtgat
    25561 ccgggtggag acggtgtgtt tgcgctccgt ggatccctcg cccttcatgg agctgtccga
    25621 tcgaaatccc agggccgaca ggatcttgcc caaaagcccc acgccgtctt ccaggttgga
    25681 gctattggtc ttggtcaggt cggtcttggc ctcatccttc acggaggtcc gttcgctcac
    25741 caccacggtg acgatatctc ccacggcggt ggggcgggcg tcgctaaaca ggctggcccc
    25801 ctcccgccaa agagattctc ccgaggcccc tgtcagggcc agaaacacca tggccaccgc
    25861 catgatcccg atccgtccca tggtatcact tcctctccac ccacaccgtc tctggagtga
    25921 ccagcacccc ggagaccacc gctcgactca cggtgttgcg caccctgatg gtctccccca
    25981 gggcgccccg ctgcagggtc tctccccagg cctcgacccg taaccctccc acgacgtaga
    26041 ccaggcgtac cacggcccct cggcgcacca ccggcacccg gcgcacgtcc cctaccatga
    26101 ggacggcctc ttcgggcagg gggcgaacca agatctgtcc cggccaagaa agggggtcgg
    26161 ggggagaggc gctcccctcc cactcccgga gcgccacccg ataatcttcg gggcggatca
    26221 ccgcgccgcc ttccagggcc cgcagggtca ccgccgcagg gacgagccac cggatcctca
    26281 cggggacgac gcactcccgc cccccgcttc cccgaaagcg caacaccgtg gcgcgcaccc
    26341 ccggaggcag atctcccggg cttaccaagg cgccacaggg gctcccctcg gcatggacct
    26401 ccagtcccca gggccatcgg gaaaggcgtc gcacctcggc ggtggcctct ccctcgggca
    26461 ggagctctac ccgggagggc atccgaagct ccacggtctc gcccaccagc cccgattctc
    26521 ccagggaccg ccacaggtcc tcctgggtcc atcccccttc cctgaaggga agtcgggcct
    26581 tggaggcccg ggagaccagt tcggcggccc cctccaggcg ccccacctcc ccaagataca
    26641 aggtccctcg gcgcaccgca aggggagaag ggatctccac cttgagagaa gctccttccg
    26701 ccaccccgaa ggacgccccg gccagacacc ccatccaaaa aacgacaagg aggcgggacc
    26761 aggcccgccc cctctcactg cccataccct acctccgcag gctgttggcg atccggagca
    26821 ggtcgtcggc ggtctgaatg gttttggagt tcgcctcgta ggcccgttgg gccacgatca
    26881 tgttgaccat ctcttccacc acttggacgt tggagagctc caggatgccc tggatcgtgg
    26941 tgcccatgcc cgcctcgccg gggttggcga tttgaggggc accgctggcg gcggtctcca
    27001 ggaagagatt ttttcccacg gccttgagcc ctccggggtt cacgaaccga gccaattgga
    27061 gctgtcccac gttttgggga gcggggtctc cgggcatcag gacgctcacg tcccccgtag
    27121 ggctaatggc cacagccacc gcatcctccg ggacggtgat ggcgggttcc agaaggtatc
    27181 cgttggaggt gacaatctgg ccgttgccat ccatacgcca ccccccgtcc cgggtgtagg
    27241 cgatgtctcc gttcggcagc gtcacctgga aaaagccgtc accctcaatg gccacgtcga
    27301 gttggttgct cgtgaccttg aggttgccgg gggccatcat ccggctcgtg cccaccaccc
    27361 gggcccccag ccccacctga atgccgtcgg ggatcatgga atcgggttcc acgggggccc
    27421 cgggctcccg gtcgatctgg tagagcaaat cttcgaagtc cgcccggatc ttcttgaacc
    27481 ccgccgtgtt gacgttggcc aggttattgg acaccacgtc caaattggtc tgctgggcga
    27541 tcatgccgct ggccccagcc cagagagagc gcatcatggt aaataccccc tgtcctttcg
    27601 ccctagaccc ggccgtaggt ctgggtcagg cgcgaggccg attcgtcctg caccgacacc
    27661 gccttggccg ccgcttcgta ggcccggttt gcttcgacca tgcgcaccat ctcggagacc
    27721 acgttgacgt tggacatctc cagggcgccc cgggccaacg tggggttggc ctcttcctgg
    27781 ggagcgccgg aacccgccgt ctcctgcagc aaagacctcc ccgcctggcg caggtaggtg
    27841 gggttgtcga aggtcaccac ctgaagccgg tccaccactt ctccatccac caccacttgg
    27901 cccccatcgt caatatgggc ggtccatccc tctcccaggg ttatggtgcc cccttgcccc
    27961 tgaaccagct gcccttcgtg ggtggccaga cgcccttcgt tgtcccggac gaagtgcccc
    28021 gcccgggtgt agaaggtatt gcccgccccg tcggccacca cgaaaaaacc cggaccttcc
    28081 agggccacat ccagggggtt gtcggtgacc cgcaccgccc ccgcctggat cgacatggcg
    28141 gtctcggaga gcacattggc gaacaccgca tcctggatgg gatcccgccg gcggagccac
    28201 tcgggcttag gagggaaggt ttcccccggt tgtccatcga ttttttccat cagaagcgtg
    28261 ggaaacgcct tgttgacggg agtccgacct ctaaatcccg tggaattcac gttggccaag
    28321 ttgtttcccg tcacgtccag catgcgctcc tggaccagca tggccgacgc ccctgcgtac
    28381 agaccgcgga acacggcctc acccccctct agagatcacc ggaccggaga aagccctccc
    28441 ggagtgtcat cgggggcgcc gtcccccttt gcgcgacgtg gaaaggattg ttcccgagtc
    28501 ctttagttta tcgagttctt ccatggcctt gcccgtccct agggccacac attccatggg
    28561 ttggtccgcc acgtggcagg aaatccccgt ctgttcagtg acgagctccg gaaggttgcg
    28621 caacagggct ccccctccgg tgagtaccac ccctccctcg ataatatcgg ccgaaagctc
    28681 cggaggcgtt agttccagga cgcgccgaat gccatccaca atggactgta cttgctcgga
    28741 aatggcttcg gccacccccg cgctggtgat ttggatctcc cggggcaatc ccaatacgag
    28801 atccctaccc cggatgttca tggtcataac ctctccccgg gaatagcagt tgccgatccg
    28861 gaccttcatg tcttccgaag tctgctcccc tatggccagg ttgtagttcc gcctgacata
    28921 gcgggatatg gcttcgtcga acttgtctcc ccccaccctg agggattcgg agatgacgat
    28981 gttacccaaa gagatcacgg cgatgtccga ggtacctccc cccacgtcca ccaccatgtt
    29041 tccccggggc tctcccacgt tgatccccgc tccgatggcc gccgccatgg gctcttctat
    29101 gaggtaagcg tctcgggctc ccacttccag cgccgcttcc agcaccgccc gacgctccac
    29161 gtccgtggcc cccgagggca cacagaccat tacccggtgc cgaaagaatc gccccgtccc
    29221 ccgggtaact tttttgatga aatggcgcaa catggcctcc gtcatggtgt agtccgcgat
    29281 gaccccgtcc cgcaggggtc ggatggccac cacgtggccc gggaccttac ccaccatgcg
    29341 cttggcatct tctcccaccg ccaggatgcg gttattgtct ttatccagag ccaccaccga
    29401 gggttctcga agcacaaccc ctttacctcg tatgtagatc aggacggtgg ccgtcccgag
    29461 gtcgatgccg atgtcgttcc caaacacgct ccgctacccc cttttcgtcc cttcgtcgag
    29521 aatggagcgc ccacacccct tggcgcccag ctcattttcc ccaacttttt ccgaaactcc
    29581 ggcttccgcg agttccggcg ggccggatca aactataggg ctttctccct tctcccacaa
    29641 gagcttttcc ccctcatccc cgtaacccac gttccagaga aaaagcccct ggggaggggc
    29701 cgtcatgccc gccatggccc gcgaatggcc ttccagcagc gaggcaaacc acgccaccgg
    29761 tcgcctcccg cgccccacct ccgccaggct tcccactaag atgcgcacca tgttggtgag
    29821 aaaggcgttc cctcgaacgc gaaaccacca gagggatccc cggcgggcca gacgggcgga
    29881 aaagatcgtc ctcacggtgc gttcggggcg gtcggcggac cgacagaagg cggaaaaatc
    29941 gtgagtcccc ggcaaaagga cgcaggcctg acgcaccatt tcccagtccc agggctggtg
    30001 aatccaccac gtcatggtgt ggagatgggg ataggtcgcg ggcgctgccc agaggaaata
    30061 gacgtactca cgccaccggg cactgtggcg ggcatcaaaa tccggcgaaa cgggggagac
    30121 ccccagggcg cggacgctct ggggcatgcg agcgtccaag gcgcgccgaa gcatttcggg
    30181 ctcccaggaa cgggggacct caaacgacac cacctgaccc cgggcatgga ctcctgcgtc
    30241 ggtacgcccc gcagcgctta tgggaaccaa aacgccgtgg agcgccccca gggcttcttc
    30301 caccacctgt tgaaccccga ggccatggcc ctgggcctgc caaccggaga aggccgcgcc
    30361 gtcgtaggcc agtcgcaccg cgtatttcat cgcagcaggg gccaccgggc atccagtacg
    30421 cagacgaata ccgtcactag gcacaatccc actagggctc cggagtcccg gagggtccac
    30481 ctcaaggggt gcatccgggt gcgcccgtct cccccccggt agcaccgggc ctccatggcg
    30541 gtggccagat cttcggccct ctgaaagacc agaataaaaa ggggcaccaa cacggggacg
    30601 aaggccttta tccgacgccc tatccccccc cggtcaagat ccgcccctcg ggcgagctgg
    30661 gccttgagga tgcgatcggt ctcatccaat agggtgggga taaatcgaag ggcaatggtc
    30721 atcatcatgg ccatctcgtg ggcgggaaaa ccgatgggct ttaatatgga gaggaggcgc
    30781 tccaagccat cggcgagctc catgggggac gtggtaaggg ttaaaaatcc cgcaaaaagg
    30841 accagaaaaa acagccgaaa gctcatggta aaagccgcca ccatcccttc tcggctcact
    30901 cgcaggatcc cccaggacca cagaggttcg ccggaggaaa aaaacaggtt gatcacggct
    30961 gtgaaggcga tgaggatgac cacagggcgc accccccgga gcgccatgga aacggggatt
    31021 ttagaggccg acgcgaggag aaaaaagagt cctccccagg cccccatggc ccccgcgcca
    31081 tcgcccacaa aaagggcccc catgagagcc accgtgccca ggatcttgca ccgagggtcg
    31141 aggcgatgga ctaaggagtc cacggggatg tactgtccca aggtcatgtg atccaggaac
    31201 ttcaatatcg cccccccttt tccagcctcc gccccagctc ttcggcatcc atcgtcaagg
    31261 ggacggccag gccccgctcc cggaggcgcc gggagaaatc cagcaccggg gggataatga
    31321 gtcccggcag gacggctcgg cacaggggat ccaccaggtc ttggggggaa cccatgccaa
    31381 ggacgcgccc gtggtccaac accaggatgg catcgcaccg cgagagggcc agctccaggt
    31441 cgtgggtgat ctgcaatact cccatgcccc cccgcaccaa ctccgctatg aggctcaaaa
    31501 gatctcgtac gccgccgcca tcgagccccg ccgtgggttc atccaacacc aggtactcag
    31561 gctccgccgc caagacggag gccaaggcca cccggcgctt ctgccccccc gacagggaga
    31621 aggggcttcg ggaggataga gatgtttcca gtcccacttg agccagggat cgggccacga
    31681 gggcctccag acgcccctcg tccacccccc agtttcgggg ggcgaaggcc acctcatcgt
    31741 agacgttttc cgcgaagagc tgctgttcgg ggtactggaa aaccagcccc acccgccgcc
    31801 ggacttctcg gagatcgggg ctgcgatggg tcaccgtcat gccgtccacc gtcacttttc
    31861 cctcctgagg aaggatcagg gcgttaaggt gctgggccaa ggtggactta ccgctccccg
    31921 tggggcccac cacggccatc cactggccgg agttcatctc gaagctcaca gagtcaaggg
    31981 cccgccgggc caggggggtc cccgcgtggt aggtgaacga gaggtctttt accgagaggg
    32041 acatagggcc tccgccattt ccttccctcg gggaaagatc gtccggggga tgcgattccc
    32101 gctcctgagc ctgcgccaga ggcgccacag gggggggtct tccacgcccc actcggggcg
    32161 gggcaccgcg aaaaagtcta gcggagcccc gtgccaaacc tgctggcctc ggtgaagcac
    32221 gtggatttcg tcgcaatcca aaatttcttc caaccgatgg gtgatggaga caagggtcat
    32281 gccctcggca tgaagctctc ggagcaccga cgccaagtcc gcccggccct gggggtctag
    32341 catggaagtg gcttcgtcaa ggacaagaca gcgagggcgc aacgccagag cccccgccac
    32401 cgccagacgc tgcttttgcc ctccggaaag ggcgtagacc ggctgacgac ccagggccgt
    32461 caggccgacc acctccaggg cccatcggac ccgagatacg atttcttccg agggcaatcc
    32521 caggttctcc gggccaaagg ccacatcttc ttccaccgtg gccgccacga tctgattttc
    32581 tgggttctgg aacaccatac acacttctcg gcggacagcg gcgtggtgct cttctttgcc
    32641 cgtgtcccat ccacagacaa tagaagagcc ctgcgtgggg accagcaggg cgttgaagat
    32701 gcgcgccaac gtcgattttc cggaaccgtt gctccccaaa agggccaccc aggcccccgg
    32761 ggcgatccgc aacgatatgc cggcaagggc tggggttttg gctccgggat aggtataccc
    32821 cacctcccgg agctcacaga ccgaccccat gtgaaaaatc gctactcgac cagctcgagc
    32881 accaccatgg gggacgcatc tcccacgcgg ttaccggtct tgacgatcct cgtgtatcct
    32941 ccgggacgcc cctgatagcg aggggcgatt tcatcaaaga gcttgcatac cgcctctttg
    33001 tgcggcatac gggcaatgac gaggcggcgg tcctgaaggg tacccccccg agcccgggtg
    33061 atcagcttct ccgccacccg acgagcctcc cgagcccgta caagggtggt ggaaatggca
    33121 ccgtgcagga acaagctcgc cgccatatta gccagcatgg catgacgatg gctcccgtac
    33181 cttcccaacg tgctgtggtc cacgcgatgt ctcatcgcgc tattcctcct cctggctgtc
    33241 ctgtgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntaaag ctccgccgcc
    33361 aagcgggccc ccacttccag ggtgcaaatg ggcgcttcgg gatcgacaaa atccacatcg
    33421 ctgtcccgtt ggacatccgc cgccttgacg tctttggggc cttccaggtc tatgcgaagg
    33481 atccggattt cgtcgctgtg ggatcgcacc ggaacgtgct tgaggttcag cagaagctcg
    33541 atgacgtctt cgcgcacccc cggcacggtg ctgaattcat gcaacacgcc atcgatccgc
    33601 acggcggtaa tggcagctcc gtcgatggat gccagcagca cccggcgcag ggaattaccc
    33661 aaagtcaccc cgtacccccg ctccagaggc tccaccacga tccgggcata ctgaggagtc
    33721 cggctgcttt cctcgacacg aatttcggtc tgcaagtttt ccacgagcgg attccccacc
    33781 ctccaccgct acttcgcggc gtagaattcc accacgagct gttcgttcac cggcgaatcg
    33841 atctgctccc gcgtgggaag agccaccacg gagccctcca tgcggtcggc gttgaaggtc
    33901 atccaggcgg ggatggcacg ggatcctgcc acttcagcgt tctctttcag caacaccaga
    33961 tcgcgactcc cctctctcac gaggatcacg tctcccgcct tcaccaagaa actggccaca
    34021 tcgaccttgc gtccgttcac cgacacgtgc ccgtggcaga cgatctgtcg cgcctggctc
    34081 cgactgactc cgagtcccat ccggtaaatg acgttgtcca ggcggcgctc cagggtctgg
    34141 aggaagttgt gccccgtctg ccccgccatt ttgaccgcta tgccgaagaa acgccggaac
    34201 tgcgcctctc ccatgccgta gaaccgacgg agcttctgct tctcccgcaa ccgcaacccg
    34261 tactcgctag tcttaacccg cttggcgccg tgctgtccgg ggcgcacgtt gtttttggag
    34321 atggcgcact ttcccgtata acagcggtcg cctttgagga agagcttcac gccctccgca
    34381 cggcactggc gacacaccgc tcccgtgtat ctgctcatgg ttggtccttc tcctccttaa
    34441 acgatgccct agacccggcg ccgcttagga gggcggcaac cgttgtgcgg gatcggcgtt
    34501 tcgtccttga tcatgttgac ctgcaaaccc gccgcctgca gcgagcgaat ggccgattcg
    34561 cgtccgggcc cggggccttt caccaccacg tccagctccg ccagaccaca gtcctgcgcc
    34621 accttggccg ccgcctgggc cgccatctgg gccgcaaagg gcgtggactt tctggtgccc
    34681 ttgaacccca cgttcccccc cgacgaccag gaaagcaggt tgccctgttt gtccgtgatg
    34741 ctgatgatgg tgttattgaa cgtggagtga atgtgggcca ctccataact gacgtgcttt
    34801 ttctccttgc gcttgcctct gcgctgcacc ctcttggcca taccttacgt ccctccttgg
    34861 gctccggcgc cttacttcgt ggctttcttc ttgcccgcca cggtacgacg gggccccttg
    34921 cgcgtgcgcg cgttggtctt cgtccgctgc ccccgcacag gaagcccctg cttgtgtcgc
    34981 agccctcgat aacatccgat ctcaatgagg cgcttgatgt tcatggcgac ctcacgccgg
    35041 agatctcctt ccaccatgtg ggagttttct atctcggccc ggaggcgctg ggcctcttcc
    35101 tccgtcaagt ccttcacccg agtgtccgga gacactccgg tgacgcgcaa aatcttctgc
    35161 gacgtcggca aaccgatgcc gaaaatgtag gtcagcgcga tctccacccg tttgtcgcgc
    35221 ggcaggtcca cgcccgcaat acgagccatg ccgtcttacc tcctcgctcc ttgacgctgc
    35281 ttgtgacgcg gattcttgct gcagatcacg cgcaccacac cgtgcctgcg aatgacacgg
    35341 caatgttcgc agatcgtttt caccgcaggt ctcaccttca taccttttca cgctccttgc
    35401 cgaaaacacg caccagccgc ggtacgtacg taagccctct aagccttagg cccccctact
    35461 tatagcgata gacgatccga ccccgggtga ggtcgtaggg ggacaattgt accagaactc
    35521 ggtcgccggg caaaattcga ataaagtgca tccgcatctt tcccgacaca tgagctaaaa
    35581 ccttgtatcc attctccagc tccacccgaa acatggcgtt cggaagaggc tccgacacct
    35641 taccccgcac ctcgatgaca tcatctttgg ccatgcgccc ctccacctat cctcgacctg
    35701 agccgaacct gctgaaaatg cgggactacg attcccatgg ggtcagcacc tccaccccgt
    35761 cctcgagaac ggccaccgtg tgctcgaagt gggccgcatc cgacccatcg gccgtgacca
    35821 ccgtccaggc gtccggtttg gaaactactt cttccctccc cgtcatgacc atgggctcta
    35881 tggcgatggt catgcccttc ttgagggtga tgcccgcccg ggggcgcccg taattgggca
    35941 cctggggggc ctcgtgcaat tttttgccga tcccgtggcc cgcataggtg cgcaccagtc
    36001 ccaaacccgc ctggagcacc cgggattcca ccgcatgtcc cacatctccc agcgttcgcc
    36061 ccggccgtac caccgccaag gcggcgtgga gagacgccag tgtcacttca agcaagtgtt
    36121 gcctttgggc ggaagtcttt cccaccgtgt acgtacaggc cgcatccccg tagtaaccct
    36181 ggagcagaac tcccacatct acgctgacga tatccccctc ttcgaggcgg cgcacccccg
    36241 gtatgccgtg gaccacttcc tcgttgatgg acacgcagat gcttccggga aaggggcgag
    36301 aaatacccgt cataacgtac cccttaaagg ctgcggtgcc cccggaacga ccgatcagtt
    36361 catcggcccg acggtccagc tccatggtgg tgatgccggg ttggacgagg tcccgcatga
    36421 ggcggagcac gtccgccacc accgccccgg catgccgcat gacttgtaag tctttttcat
    36481 ttttcagaga gatcattgcg atctttcgga cgatcccctg gttccctgaa ggtcctggga
    36541 agccagcacc cgaagcaccg cgtctcgcac gcccccatcg acttcggcca gaatatcctt
    36601 ggcacgataa tacgccacca ggggcgtggc ctggtcgtgg tatacctgaa ggcggtggcg
    36661 aatgacttct tcgcggtcgt cgtcccgctg gatcacggct ccgccgcagg cgtcacaaat
    36721 cccctcctgt ttggtcgggt ggaaagtgac gtggtagatg gcgccacact gcttgcagac
    36781 ccgacgggag cacaggcgct tcaccaccac ttcgtccgtc acttggaaca aaatcacctt
    36841 atcgatgtcc tgcccctggg cccccagggc cagatccagg gcttccgctt gggccaccgt
    36901 ccggggaaaa ccgtccagaa taaagccctg acgacagtct tcgtctccca ggcggttttt
    36961 gatcaggtca atcatcactc cgtcgggaac caacttcccc gcatccatgt agcttttggc
    37021 ctggagcccc aggggagtgc ctcgcgccac gtgagaccgg aggatatcgc cggtggannn
    37081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatg tccacggggt tgaagaccac
    37201 cgccgtgtag aagtaggcga aaaacacgat gaggatcacg tagagcacca ggtacaccgg
    37261 acttcccgga gaaaagaggc cctttagcat ctgggcccat tcccccgaaa agaggttcag
    37321 gatcgtgtac ggaaagagca gcacggagga ggcgaagatg atggggatca ctccggactg
    37381 gttcaccttc aaggggatga aggtgctctg gccgccgtat accttgttgc ccacgacccg
    37441 cttggcgtac tggacaggaa ggcggcgctg cccctcttgg agaaggacgc accccgccac
    37501 cacgcccacc atgaggacga gtcctaccag catggccagg gcgctcatct ctcccatgcg
    37561 caaaagggtc caggtctgga cgatggcctc agggattcgc gccacgatgc cagcaaagat
    37621 gagcatggaa atgccgttgc cgatgccgtg gtccgtcact tcttccccaa gccacatcac
    37681 cgccacggac cccgccgtca ccgtgagagc caccaccacg ccgtccagga tgtttccgct
    37741 gaagataccc atgttgccca accagaaggt catgcccacc gcttgaacta gggcgaaaag
    37801 caccgtgccc caccgggtat acatgacgat cttcttgcgc ccgtcttcgc cctcatgctt
    37861 ttgcagcttc tccagggcgg gcaccaccac taccaacagc tgcatcacaa tgctggagtt
    37921 gatgtacggg gccaccccca aggcaaagac gctgaaccgc cgcaacgccc caccggcaaa
    37981 gagatcaagg aagtccagag ctccaccctg ttggaacatg cgggccatgg cctccggatc
    38041 aatgcccggt gtggggatgt gggctcccag ccgaaaggcc aggagcaccc ccaaggtgaa
    38101 caggatcctc cgcttcagat cgggcaagcg aaaggcgtcg cggaaggaat cgatcaccta
    38161 gatcacctcg gcctttccac ccgccgcttc gatctttcgg agggcttcgg cgctgaacgc
    38221 atgggctcgg accgtcatcc ccttggtgag ctctccctgg gctaagatct tcaccgggag
    38281 ctgatcgtgc ctcacgagaa gagcccgacg gagggcctcc acatccacct ctgcccccgc
    38341 ctcaaaacgc tcttccaggt ccaccaaatt aaccacttgg tagtttttgg cgaaccggaa
    38401 gttgttaaaa cctcttttgg ggatccgccg ggagaggggc atctgccctc cctcaaaacc
    38461 cgggcgcact ccccctcccg aacgggactt gtgaccctta ttccccttgc ccgccgtctt
    38521 gccgttgccg cttcccagcc cctggccctt gcgctttgcc acgtgatggg agcccttggc
    38581 ggggcgcagc tcatgcagct ccatggagcc cttccccctc tctactcggg tacttccgac
    38641 cactctacca ggtgggacac cgcacggatc attcccctga cctggggcga atcctcatgg
    38701 accaccgtgt agtgaagccg acgcaggccc agggctcgaa tcgtccgccc ctgccgctcg
    38761 ggatgaccga tggcactccg tttccacgta atacgcagtt tcaccatggc cctcctctcc
    38821 ctacgcttct ttgcggtcca gcccccggag gcggcgaatt tcatcgggag tccgcatgat
    38881 cttcactgct tccatggtgg cataggccac gttgatggag ttcgaggtgc gtccgatcac
    38941 cttggtcagg acatctttca ctcctccaag ctccatgatg gcacgcacca cggctccggc
    39001 gatgactccc gtaccgggag cggcgggctt tagaagcacc cgagccgcgc cgaagtctcc
    39061 gataacaggg tgagggatgg tgtgccccac tttcttgagg tccaccagat ttttcttggc
    39121 cttttcgatg gctttccgca ccgcctcgga gatttcccgg gccttgccga tccccacccc
    39181 cacaaagcgg gtgccgtccc ccaccaccac aagaatactg aacttgaagc gcttaccgcc
    39241 cttgacgacc ttgctgacgc ggttaatgga aacgacccgc tcggacagct cgacgccctt
    39301 ggcgctgatg gtcctattag ccatgagctc tcctccctta gaaatcaagg cccgcctccc
    39361 gggcggcatc ggccagagcc ttcacccggc cgtggaacat atgaccgccg cggtcgaaca
    39421 ccaccgcctt gatccccttg gcgagggccc gttccgccac gagacgcccc acggtgcggg
    39481 ccgtctcggc gttccccgtg cgccccccca gggtggcgcg gatatccggt tccacggaag
    39541 acgcggccac cagcgtacgc cccgcctcgt cgtcgatgat ctgagccacc acatgcttca
    39601 agctccgaaa gacgctgagc cgcggacgat caggggtccc cgaaagcgtt cggcgaagcc
    39661 tctcgtggcg aacctgccgc atctcgtttc tgctgcgttt ttcaatcaag gctgctcacc
    39721 tcccgcctac ttgctcccgg tcttgccggc cttgcgaatg atgacctcac ccgcataccg
    39781 gattcccttg cccttgtagg gctccggggc acggaactcc ctgatgtttg ccgccacttg
    39841 ccccaccgcc tgcttgtcga tgccccggac gaagatcttg gtggggccgt ccgtggcaat
    39901 ctcgatcccc gtgggtggat cgaactcgat gggatgggag taacccaggt tgagcaccag
    39961 ggtcttaccc ttcatctggg cccgatagcc cacccccacg atctccaaga ctcgctcaaa
    40021 acccgaagaa actccatgga ccatattgct gatcatggcc cgggtcatgc catgcagcgc
    40081 ccggctcgtc tttaggtcgt cgacgcggga aacggtggcc acgtcgcccg ccacctgtac
    40141 tccgacctgg ggaaggatgt ccatcgagag ctctcccttg gatcctttca ccaccacccg
    40201 ggccccgtcg acgttcaccg tgaccccctt aggaatagaa atggctttgc gcccgatccg
    40261 cgacataacc ctttcctccc taccacacga aacaaagaac ttcgccgccg agcccctgct
    40321 tcctggcgtc ggcatcggtc agaagccccc gagaggtgga aaccacggcg atacccagtc
    40381 ctcccatgac cttggggagc tctcccacgc ccatgtaaat gcggcgtccg ggcttgctga
    40441 tcctcttaat gccctgcatc accctttccc ggctgggtcc gtagtgcaaa aagagccgga
    40501 gcacggggaa gggcttcttg ggatcgttga taactttgaa attacggaca tacccttctt
    40561 ctttgaggat cttggcaatg ccgactttga tcttgctaac cggcagatcc accatctcgt
    40621 gatacgccaa gttggcgttc cggatgcggg tgagcatatc cgcgatggga tcgttcacat
    40681 acatggcctt ggcctccttc gccgtacccc gcctaccagc tggacttcac gacgccggga
    40741 atcttgcctt cccgagccag ctttcggaag cagcaacggc acatgttgaa atcacgcatg
    40801 aaggccttgg gacggccaca aagcgggcag cgattatgct tcctcacttt aaatttgggc
    40861 tcctgctggg ccttgtactc catggctaca cgagacattc gccaaccctc ctgcctaccg
    40921 cataaacggg aaaccgattt cggaaagaag ggcggctcct tcttcgtccg tacgggcggt
    40981 ggtggtgatg gtgatgttca ttccacggac ccgaatgacc ttgtcgtagt ctatttctgg
    41041 gaagatgagc tgctccctga ggcccaggtt gtagtttccc cggccgtcga agctcttacg
    41101 actcactccc tggaagtcct tgatgcgagg gagggtcagg gaaacgaggc gatcaaggaa
    41161 ctcccacatc ttattccccc gaagcgttac gaaacacgcc accggcattc cctcacggac
    41221 tttgaacccc gccacggact tcttcgcccg acgcatcaga ggacgctgtc ccgtaatgat
    41281 gccgagttcc tccatggata tgttcatgta cttaatgtcc tgcttggcct cgttcacacc
    41341 gatgtttacc atgactttct ctacgcgagg cacctgcatg acgttcttgt aaccgaactt
    41401 ggaggacagt ctcgggacga tctcttccct gtatttttgc cttatgcgcg gaatcatgct
    41461 ttcgtccctt cccctcagac tttgtccact acctcgccgc actgccgaca caggcgcacc
    41521 tttttgccgt tatcgaggta ggcgtgcccc actcgcgtcg ccttgccaca ggccgggcag
    41581 acgagcatgg ccttgcaggc gtaaaagggc gtttcctgct tgacaatgcc gcttttgggg
    41641 ttcttcttgg aggggcgcac gtgcttcgtc gccatgttca cgccctccac cacaatcatg
    41701 tcccgatcca cgtcccgccg aaggatcttc ccttctaccc cggcatactt tccggaaatg
    41761 acgcgcaccc ggtcaccttt tttcagacgc atctttccca tggctccggc cctcctaaac
    41821 cacttccggc gccagggaga ctatgcgcat atactttttc tcccgaagct cccgggctac
    41881 ggggccaaag atgcgcgttc ccttggggtc gccgttgccg tcgatgatga cggcggcgtt
    41941 gtcatcgaaa cgcacgtagc tcccgtcgcg ccgacggact tccttctttg tgcgcacgat
    42001 gacgcatttc acgaccttgc ccttctctgt gttgctgtta ggaatggcct cccgtaccga
    42061 agcgacgatg acgtccccca cggtgcccac tttacggaag cttcctccca ccacctggat
    42121 gcaggtcacc ctcttcgcac cggtattgtc ggccacattc aggaccgtac ggagctgaat
    42181 catgtcctat gcctccccgc cgtcgctgcc cgtgaggatc ggagcgcgct ccagcacttc
    42241 cgccagagtc catctcttat gcctactcag gggacgggtc tccacgatac gcacccggtc
    42301 tcccatgcgg caggtgttct cttcgtcgtg cacctgatac tttttcgcgt gaataacggg
    42361 cttgccgtac aaaggatgct tggccatccg gtctacgcgg accaccacgg tcttttgcat
    42421 cttcgtgctg acgacgatgc cgtcccgcga cctcgcgcgg gaaacgttct cttccatcgt
    42481 cccttacctc ctcgccggtg cgcggtccag gccgagctct ttctcccgga ccatcgtcag
    42541 gatacgagcg atggtccgct tgacatcgcg aatccggccg gtgttcccca gctgcccaat
    42601 ggcatgctgg aaacgaaggt taaaaagctc ctctttatac tgccggtgct tgccttttag
    42661 ctcatcaagg gtcaaatcgc gcatgtcctt ggtgtccatt attcttcacc atccattccc
    42721 tcgcgggcca caagccgcac cttcaccgga agcttgtgag aggccgtacg gaaggcttct
    42781 tccgccacgt cgcgaggcac gccggagatc tcaaacatga tctttccccg cttgacgggg
    42841 gccacccacc actccgggtt tcccttacct ttccccattc gggtctccag aggctttttc
    42901 gtgtacgcca cgtcagggaa gacgcgaatc cacactttcc cgcctttttt cattttacgg
    42961 gagatcgcca cccgggccgc ctctatctgg cgagccgtaa gccacacccc ttcgagggtc
    43021 tgcagaccgt agtctccaaa ggccaccgtg gtcccaccct ttgccatgcc cttcagaggg
    43081 ctccgaaagg gcttgcggta cttaacgcgc ttcggactca gcatgagccc ctacctcctc
    43141 ttgtcccttt cccggtcccg gttcggcacg ggctgcgccg ccacgggagc ctggggctga
    43201 tccgcgccca ccgccccttt gcgaagtttg cgcgccgcat cgccccggta gatccaaacc
    43261 ttgatgccga tcacaccgta gatggtatgg gcttccccaa aaccgtagtc aatctccgcc
    43321 cgaagcgtcg aaaggggaag gcgcccctcc aagtaccact ccgtacgggc gatctctgcg
    43381 ccccccaggc ggccggaaca ctggatcttg atcccccgcg ccccggactt catggcccgg
    43441 aagatggcct gtttcatggc cctacggaag ctgatgcgtc gctccagcga ggaagccact
    43501 ccctcggaga ccacctgggc atccatgtcc gggtgcttaa tctcctggac gttgatcatg
    43561 atccgggaac ccgagatgcg ctgcaactcg tcccggacga tctggatttc ctgaccgccc
    43621 ttgccgatta ctacaccagg ccgtgcggtc cacacagtaa aacgcaaaac attcccgata
    43681 cgctcgatct ccaccttgga gaccccggcg tggaaccagc gcttcttgat ccaatcccga
    43741 agcttgaggt cttcatggag atgcttgaca taggtcttac cgctagcaaa ccagcgggac
    43801 tcccaatccc ggataactcc cacacgatac cctaccgggt gcaccttctg gcccaaagtc
    43861 tcccctcctc tttaacgttc agccacggcc acggtgatat ggcacgtgta atggcggaag
    43921 ggatgggcac gccccatgga aacaggacgg aagcggcgca acgttgtccc tgtatccgcc
    43981 accgctttga cgaccacgag cttgtccacg tccatgccaa aattgtgatc cgcattggcc
    44041 accgcgctat taagtatctt ttcgatgaat ctcgcggctt ttttgggcgt gtaccggagc
    44101 gtcatcacgg cgtcgttcgc gtttttcccg gcgatgagcc gaagcacctg tctagccttg
    44161 ttcggggaaa tgcgcacctg cttcgccatg gcatgtgctt cccggatctt atccatgttc
    44221 cgtcctcctt atttaacgcg ggaggtgcgt tcttgccccg cgtggccgcc gaagcgccgg
    44281 gtgggagcga actccccgag cttgtgcccc accatattct cgctcacaaa caccggcata
    44341 tgggtctttc cgttgtgaac ggcaatggtg tgtcccacca tctcgggaag aaccgtggat
    44401 ctccgggacc aggtcttcag aacccgcttt ttccccgagt gattcatatc ctcgattcgc
    44461 ttcaccagtt tcgcgtcgac gaaaggcccc ttcttcaacg atctggccat gttccgtcct
    44521 cctccgccct acttctcgcg ccgacgcacg atgtgcttgt cggactgctt acgcttcctc
    44581 gtgcggtatc ccttggcagg ggttccccaa ggcgacacgg ggtgcttgtt tcccttggtc
    44641 ttgccctcgc cgccccccaa ggggtggtcg caggggttca tcaccatacc ccggacgtgg
    44701 gggcgacggc gaagccagcg gctcttgccc gccttgccga tggtggtgtt ctcatgatct
    44761 tcgttgccca ccaggccgat ggtggccatg cactccagca ggatgaggcg aagctcggag
    44821 ctgggcatgc ggatgaaggc atatttgccc tcttttgcca tgagctgggc ccccgctcca
    44881 gcggagcgca ccatgacacc accccttccg ggctgaagct caatgttgtg gaccacagtg
    44941 cccaacggga tgtcggccaa cttcagcgca ttgcccgggc ggatgtccgc cttagggccc
    45001 gccatcaccg tatctcccac gctaagccct tggggcgcca ggatatagcg cttgtcgccg
    45061 tcaagatagt ggatgagggc aatgcgtgcc gtccggttgg ggtcatactc aaaggccgcc
    45121 accgtgccgg ggacgcctat cttattccga cggaagtcga tgatgcggta cttgcgcttg
    45181 gcgcccccgc cccggtgccg catggtgatg cgcccggtgt tattccgtcc cgcgaagttc
    45241 ttgagagact ccaccaactt tttctcgggc ttgcccttcg tgatctcctc aaaggcggag
    45301 atggtcatga aacggcggcc gtcggtggtg ggcttgaatt tcttgagtgc catcgtaccc
    45361 tgtcctttcc gtccgtcagg cgttcgcgcc ctcgaagaat tcgatgcgct gtccttccac
    45421 caacgtcacc atggccttct tccaggagcg ggatttcccc aggaacgccc ccatacgctt
    45481 gggcttgggg tgaacattta tcgtatgaac cttggacacc ttgactttga agaccttttc
    45541 cacggcgtta cggatctcaa tcttgttggc gtccggaagg acttcgaagg tgtaggtgcc
    45601 gtgctccatg agcgcgttgg acttctccgt cacgatgggg cggactatga cgtcatgatc
    45661 gctccgtccg atcatcggga aaacacctcc tctaccttgc gcgccgcctc gggcgtcatg
    45721 atcagatggg catggttcag cacgtcgtaa acgttcaggc tgtcgacgtg gagcaccttg
    45781 gcccccggga cattggccgc agagcgatac aggttgacgt tggtctcgtg caccaccaac
    45841 aggggcttct tctcggctcc cactttggag agaaaggcca ccaccgcttt ggtcttcggg
    45901 gcctccagca cggggttttc cagaagcatc atgcgatctc cccgcacctt gagcgacaac
    45961 acgctccgca tggccagggc ccgaagcttc ttgttaacct tctgggagta atctcgggga
    46021 tgggggccat gggccacccc gccccccacc atgacggggg aacgaaggct gccccggcga
    46081 gcccgccccg tgtgcttctg ccggaagagc ttgcggttgc ttccccgcac gtcgctccga
    46141 tccttggcgt tgtgcgtccc ctgccgggcg ttggccagct gggccaccac cacctggtgc
    46201 atggcagcca tgtgtaccgg agccgcaaaa acggcgtcca agagctcccg ttcacccagg
    46261 gaggttcctt cgaaatcaac aatcttaacg ttcggcatag ttccccgtcc tccctacgcc
    46321 tcgtgcttgt ggaccatgac gaggccgttg cgggcgccag gaatcgatcc cttgatgagc
    46381 aaaaggtggt tttccgcatc cacggcaaaa acagtgaggt tcttcaccgt cacccgttcg
    46441 tcccccatgt gccccgccat ggtctttccc ttgaaaacac gccccggata ggtgtggcat
    46501 ccgatggaac ccggccgacg gtggatcacc gacgcgccgt ggctcttggc tccgccaccg
    46561 tagttgtggc gcttcatgac ccccgcaaag ccctttccct tgctgcgccc cgtggcgttg
    46621 accttttctc ccggggtaaa aatttccacc gtcacatatt gccccagttc atacgccttt
    46681 ggatcccgaa gccggaactc ccgaagccaa cggcgaggct ccacgcctac cttgtcgtag
    46741 tgcccctgaa ggggtttgct aacccttccc tttttgatgg ccccaaagcc gagctggatg
    46801 gccacatatc cgtttttctc cggagtcctg atggccacca ccggacaggg ccccgcttcc
    46861 accaccgtca cgggaacggc tcggccgttc tcgtcgtaga cctgggtcat ccccagctta
    46921 cgtcctatga ttcccttgct catcgcgcgt agccctctcc tttcagctca gcgctccccg
    46981 ccttagagtt tgatctggat atccactccg gaggggagat tcagctgcat caacgcatcc
    47041 atggtccgtt gcgtgggatc caaaatatca atgaggcgct tgtgggtgcg cacctcgaac
    47101 tgctcccgag cgtccttatc cttgtggggc gacttgagga tggtgtattt ttttatctcc
    47161 gtaggcagag gaatggggcc cgccaccttg gctccacttc gcaccgccgt ctccgagatc
    47221 tggagcgccg aggtgtccag aactcgatga tcgaaggcct taagtttgat gcgaatcttt
    47281 ttgctcacag cactcacgcc ttccgcgcaa cctagtcgag aatctgggtg acgacgcccg
    47341 ctcccaccgt gcggccccct tcgcga
//
LOCUS       DAOOER010000010        44825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_894_length_44825_cov_9.445276, whole genome shotgun sequence.
ACCESSION   DAOOER010000010 DAOOER010000000
VERSION     DAOOER010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 44825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44825
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_894_length_44825_cov_9.445276"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="PL162_02600"
     CDS             complement(<1..1205)
                     /locus_tag="PL162_02600"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HQF90954.1"
                     /translation="MKRLLEGQDVLGVMPTGAGKSVCYQVPALLRPGVTVVFSPLISL
                     MKDQVDALRQSGVAAQALHSSLGEGEFREVVLALRSGECPLIYVAPERLESATFLKLL
                     ATLPVEFFVVDEAHCVSQWGHDFRPAYLAIASALKALPRRPPVGAFTATATPEVRKDI
                     EKKLDLRSPFSLTTSFDRPNLLLRVERPEDKEAFLRSFVAARPRKSGIVYCATRRAVD
                     STWDLLRRSGIAATRYHAGLEDRERVKNQEDFLHDRALVVVATNAFGMGIDKSDVRYV
                     LHYHMTKSLESYYQEAGRAGRDGEPAECPLLFDAKDVQIARFLVCQGRDREGVRRDLK
                     KLQDMTSYCRASGCLRERLLSYFGDRPPAVPCGGCGPCLGTPRRAQVVSFFRSLRPAI
                     LPHPPLSLSP"
     gene            complement(1297..1758)
                     /locus_tag="PL162_02605"
     CDS             complement(1297..1758)
                     /locus_tag="PL162_02605"
                     /inference="COORDINATES: protein motif:HMM:NF018684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HQF90955.1"
                     /translation="MTIGKNLTALALTMILSLFVWIPPAGAEERTHPIDQWVEEALAA
                     DDSTAGMRETYAEGLKRWDGELNAQYRLLAQKLRPEAMEVLKASQRLWIKYRDAEEKA
                     AQALGNELYEVNEGGTIWGVLTAERVMQITRARARELEGYVRLLTGISDEP"
     gene            2015..2965
                     /locus_tag="PL162_02610"
     CDS             2015..2965
                     /locus_tag="PL162_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HQF90956.1"
                     /translation="MAVRYKDYYAVLGVDRKATAEEIQKAFRGAARRHHPDVSKAPGA
                     EERYKEINEAYEVLKDPEKRARYDALGENWQEGQDFAPPPPRRGGKGFGSYGGQEMPF
                     GDFSDFFHVIFGGGFGVPGDFGAFSGARARPGGPRRGEDVEFSLEISLEEAQRGCRRS
                     LVWEDDGGKRQTLEVRIPSGVTEGSRVRVAGKGHPGSGGGAPGDLYLRISLLPHGRFA
                     VDGHDLRTSVEVAPWEAALGGEIPVETITGPVEMKIPPGTQGTQVFRLRGKGLPKRRK
                     DDRGDLYVTVRIAIPRQLSSRERELFEELARVSTWKPRGE"
     gene            complement(2976..4601)
                     /locus_tag="PL162_02615"
     CDS             complement(2976..4601)
                     /locus_tag="PL162_02615"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HQF90957.1"
                     /translation="MAECGSDHDRDRQRLLDLFQQKGFRLIFEEAPFAIALKDRHNHM
                     LRANPYVERLTGKVSTAAEGLSMEELFPQEGARFFDQDREVLRTGSPLYGLLQRYETP
                     SGELLWTLADKIPFRDEEGAVAGILVFERDITEYKRAERIQAALYHISETARGEAPLE
                     ALYADVHRILADLMPAKNFYIALCDREQTTVRFVYHVDEKDDLSEEEWHGVPLQPLGL
                     SFRVIQKGTAILYDHAACMAMKERGQLRGTIPSEWLGAPLRAGNGEVFGIVAVQTYDE
                     GVRYPPRAMEILNFMATQVANALEHRHHAEALAASQRELARNVAQLQKSWDQTLEVLS
                     RTSEAHDIYTVGHQSRVAELAAAIASLMHFSPERVHGVRMAALVHDLGTITVPSEVLC
                     KPGVLSEDEYALVKLHPSQGQEILKRIELPWPLGAMVLQHHERMDGSGYPQGLRGEEI
                     LPEARIIAVADVVEAMNAHRPYRSSLGMAAALEEISRHRGTKYDPGVVDACLAVIDRG
                     TVPWVAALRDAPPPRLARPTPEEEEDLSDLTLP"
     gene            4843..6090
                     /locus_tag="PL162_02620"
     CDS             4843..6090
                     /locus_tag="PL162_02620"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HQF90958.1"
                     /translation="MAMMLAVEDLVPGVVLHRNVTDPSGRLLLAAGVALTEAHIRSME
                     KWGVVAVQVCFGAADEDRTETGPIPPELVQETKRQLREIAGFLENKKSIPRASFHLLT
                     STLNSVIQALLDHRGELFEEVRLISHHDAHTYDHSWSVCVLSLALAREAPSVGLSGAP
                     DYLSRLSLGLGGVFHDLGKIFIPLEVLNKPGALTAEEFSLIREHPQRGMNALRTYESV
                     PPMARSIVLHHHQRWDGKGYGPASGFLLEGEEIPHLVRLVSVADAYDALVSDRPYRPG
                     YLPGDALTVLRQAGGSYFDPRVVQLLSGAVPDYPRGAVVLSKGGWVVHVDRAVRRGDP
                     LRGTVVGSLAAEGQSAVGEEVTLTSAGLLCGGTSVAHLARRLESLWHLHPSLEEHPLA
                     LHSFPPWDTLLRHHFRRGIPVMP"
     gene            complement(6137..6706)
                     /locus_tag="PL162_02625"
     CDS             complement(6137..6706)
                     /locus_tag="PL162_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HQF90959.1"
                     /translation="MTGCRLSEDQIRAAVAFHGHWCPGLALGVRAGELALEEVGRSGD
                     EDVVAVTETDMCAVDAVQVLTGCTFGKGNLIFVDHGKVAFSFFRREDGKALRFFLRPQ
                     RRDLSDGGASPEARRLRRCNEILGARLDDLFQRTEVPFILPPEAPIRETLSCPLCGEP
                     FMESRGRLLGGRSVCIPCFEAGDSRLLRR"
     gene            6892..7482
                     /locus_tag="PL162_02630"
     CDS             6892..7482
                     /locus_tag="PL162_02630"
                     /inference="COORDINATES: protein motif:HMM:NF017078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HQF90960.1"
                     /translation="MTLRRKIFFFLAAALLPVATVLFFSARYFVLDGFLQMEKSSMEQ
                     KLKWARGAFDYEKTNLADLLESWAVWDASYNYLLSPDELYEEENLNEENFKIMRLDAI
                     AYVRSDGGVVTALGCHRHSGEKGPFPAGLEAQLTSSSPLVSLSSDTKMVGILSLPGGP
                     MLVGAMPILTNLRQGPVHGVLVMGRFLDSDMAADLA"
     gene            7540..8892
                     /locus_tag="PL162_02635"
     CDS             7540..8892
                     /locus_tag="PL162_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQF90961.1"
                     /translation="MGSERPLVVVPRDEDRVTGFTLLRDIAGRPGVVFRVDQERRELQ
                     AAHRSILILGAIFLAAAVFLLGAVGLPLQRFILRPLGHMVSTAQGIAAGDLTRRFALE
                     GKDEVAQAAGAIEAMTEKLCDAMRRISETAARLDDFARNFAALADESEAGAEKSRLRV
                     EEVGAEMEGLASTAAEIDASVHEVAESAQRVAQQSADMADQVVGAQETGDAGIAALRL
                     AVAGVEEMARDATTAAASVKTLGDRARQIQGFVSQIGGIADQTNLLALNAAIEAARAG
                     EAGRGFAVVAEEVRKLAEESNTAARSIAELATAIAQDLGAVLSATERNAAAAKESQDK
                     VSGTEGRIAAMIEGLRAIAASTQDLAAVSGEQAASSQEIAASVQDIGRKVAGGTVSTA
                     EARKAMGSEVETARKVAENAHHLAQLADELQGTLAEFVFDDEEGASSASPALPFNRGR
                     "
     gene            complement(9000..10082)
                     /locus_tag="PL162_02640"
     CDS             complement(9000..10082)
                     /locus_tag="PL162_02640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQF90962.1"
                     /translation="MHGTENFPFEHDGSLVLRPAEKGELLGVARDHARIYAREHGFGE
                     DFEAYVLRGMVTYLEERHRGGSEVWVVTDGHTLAGSIGMVRHSAEEVQLRWFLVQPEF
                     RGQGWGGALLRRALEFAREQGYGKIFLWTVATLEEARKLYTRHGFHPVESVTHHLWGR
                     DLVEERWDLELPPERFPLLGHPSLPSDREPEGSCPLSHLQRRKIQAPGALALLRAFEK
                     HLGAEKAREVASAGIAEDARASGAARRNRREGAPLEVLARVVREEWAAEGALDLAFFE
                     ETPTRLRFDVLRCAYAELYEGLGAREQGFCLSCARDEAFARGLDPRLRLKRRSTIMEG
                     APRCDFLFFLEDPFSGTPARDVQTRG"
     gene            complement(10106..12052)
                     /locus_tag="PL162_02645"
     CDS             complement(10106..12052)
                     /locus_tag="PL162_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HQF90963.1"
                     /translation="MRELRGFSARFVYVYVVAMGLFHLYTAVFGNFEAYLQRTLHLAW
                     VLPLTFLLYPRRLSPCTVAGETLEAPTCEAPAPPASSAKVGGIPWYDWALAAISLLPG
                     IYGALNYGEIASRIVQVDPVTDVQLLMGGLLLVLLLEATRRVVGWPLTLIAAFFAGYM
                     YFGHYMPGIMKGLSFPIREVIEEIYLTNEGIFSIPLGVSAAYVMIFLIFGGFLEKSGI
                     GSWFMELAQALAGTQAGGPAKIAVVSSCLFGSISGSAVANVYGTGTFTIPLMKRIGYP
                     PAFAGAVEAVASSGGQIMPPVMGAGAFVMASLLGVPYKNIIIAAIFPALMYYGAVLLM
                     VHLGALKHGLRGMPSSELPDLRVVCRKLYLVIPIVGMVYLLLAGYTPMLAAVVGVALA
                     WGVSLFDPRHRMGLRDIFDAIYMGAKNIPLICIACAAAGIVVGSVSLTGFGFKFVGFI
                     FSFARDVPFLALTLIMVVSLILGMGLPTTGAYILAAALGVPLLAKLGFAPLAAHMFVF
                     YFAIISNITPPVALAAYAASSLAGSNPNTTGFIAMRLGVLAFVVPFAFCYDPGLLWQK
                     DLVGNLVSMGGGVAAIFAFGYAMMGYTNRPINVMQRGVFLGAGLLALGATVPMTLGGA
                     ALTLGGTLFWRDPRKGSLRPGKSA"
     gene            complement(12187..13167)
                     /locus_tag="PL162_02650"
     CDS             complement(12187..13167)
                     /locus_tag="PL162_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HQF90964.1"
                     /translation="MKKALGILLAGIMLVVGMAGAGGAQEGWPDQLRVMAGPPGGNWF
                     ALGGALADLWTKGTLQTTSSTGGGVSNVINTDNGKGDLGFSVTSLVGAALKGEEPFKK
                     ATPNAVVLANLYRQYTYFIMRKDYAEKNGVKSVGDVVAKKLPLRFATLKPGTASEFVV
                     KALFAKGYGVSYDDLKAWGGSVEFSSYEDGGNLLADNHLDCFAFSVGGTASTVMQIEQ
                     QVDVVILPIDEAARNALADAYGTVTFTVEPGVYKCVTEPVPVVGDYTCIVGRKDLPDD
                     LVTALCRTMWEDKGNLEKAVGDMKELTPGEAVPEKVASHPAAAAYWAGVR"
     gene            13713..15809
                     /locus_tag="PL162_02655"
     CDS             13713..15809
                     /locus_tag="PL162_02655"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQF90965.1"
                     /translation="MSTLRGRLIGVFLTVVLVALGGLGYTAFTLSREALMDLGKKEGV
                     ALAKGVAVETNFYVEGVANLLKSVASTPALQSMNWELQRPALESALKGTYAFETIFVL
                     NLSGEARTLDGVTANYGDREYFLAVRDTGKLFVSNPLVSRATGNNVVVFAVPIFGEGN
                     ALVGVLASSVNAETLMALYKSVVWGKTGYAFLVGRNGIVAAHPNQEIVGKLNASEVGP
                     SIPEALAGGVRGALAGKSSVVPYEFQGTDRMAAFEPVTATGWGVGLSSPNEEFLGAVN
                     ELRTILFLVIGVIVLVVVALSLWLAGSISRPVAAIAHHMEGVASGDLSRRITLSSSLR
                     EIRNLVTSLNEMIAAQGRTVASIRDSSREVLEKAESMSAAVEESRAAILEVASMTSRV
                     SDRTQDAAAAIEEANAGIEEVSAGAQAGAHAAVAAGEEAAAIATVARSGGEAMDAMTG
                     EIGQVAASQTRADDAMQQLGKAVTQIGSFVTTITQIADQTNLLALNAAIEAARAGEAG
                     RGFAVVAEEVRKLAEESNRAAHNVGTLINDVSSRTQAALTDNKEASTQLSALVNRSKE
                     TRQLILEMVKKVGHITESVQSIAATMEEQSAAAEEMASGMDQVAKSSQEISEAVRTIS
                     HSMEEQDAAMEIIAVASEGMVSLSQALESAVEVFKLDESSGVTPHPVAALPGAPRKIP
                     SSGAKERGASTPPKRR"
     gene            16151..16858
                     /gene="deoC"
                     /locus_tag="PL162_02660"
     CDS             16151..16858
                     /gene="deoC"
                     /locus_tag="PL162_02660"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HQF90966.1"
                     /translation="MKEFAAGGWTRKTLAATIDHTLLRPEASEAAVRRLCEEARTYGF
                     ASVCVAPCHVSLAVSCLGGPEGGVVGTVVGFPLGYQDPVIKAAEARRARDLGAREIDM
                     VLNVGWLKEGARDAVASDIRGVVEAARGGVVKVILETCLLTDEEKVAACALAERGGAR
                     FVKTSTGFGAGGATVEDVRLLRRAGGNRLEVKASGGIRTLEGALALLAAGADRLGCSA
                     GVALVEALEDSAPGAPS"
     gene            16949..18193
                     /locus_tag="PL162_02665"
     CDS             16949..18193
                     /locus_tag="PL162_02665"
                     /inference="COORDINATES: protein motif:HMM:NF006771.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HQF90967.1"
                     /translation="MSLPGAVDGERLGRRLEELGAVGRVVRGGVEGVCRLALSEEDRR
                     GRDLVISWMREDGLEVRVDAVGNIFALEPGREGEIPVMAGSHIDSVDGAGKYDGCYGV
                     LAALEALRAIRHEGGARRRPLAVGVFTNEEGVRYTPDMLGSLVHAGGMTPEEARNLRG
                     FDGTVVGEELDRMGYSGDFPCGSLIPAAYVELHVEQGPVLFREGTPLGVVEVVTGISW
                     QEFEISGTANHAGTTPMEGRRDAAVAAARVILFVRDLAREMGGAARATVGMLRLEPGG
                     VNVVPDRALFTVDLRHSDARALEEIEEKVAAFGEDVARQERVAVERRSLARFDPVPFD
                     PGVMEAIRRAVADLGMASRPMISGAGHDAQMMARICPAAMIFVPSRDGISHNPREYTP
                     PEDLERGARVLRRTLEILAERD"
     gene            18316..18963
                     /locus_tag="PL162_02670"
     CDS             18316..18963
                     /locus_tag="PL162_02670"
                     /inference="COORDINATES: protein motif:HMM:NF023166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HQF90968.1"
                     /translation="MKIYLAGCVLAAFLAGTAWGATVTYREELSGAAKTVVPVVQGSE
                     GEAGAAVNASLASLVEDQVRSLKDGDPRELIVEGRAELLEGPLWSFSFEGMVDFEGAA
                     HPSSTKKGVTFDAASGRALSLEELFLPGTDWARAVNAQLALAIDRQVAEDDLMLFDEG
                     HPNVAEYAEQFFLRPGALVVFWTDTQFAPHACGQPEFVLPRNAFAPFLRPEYASW"
     gene            19090..20055
                     /locus_tag="PL162_02675"
     CDS             19090..20055
                     /locus_tag="PL162_02675"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQF90969.1"
                     /translation="MVDSIRRMIKDLQSQGEALSGSADALAALSQESVAAAEEMRAGV
                     ESVAQRGTENRASLSRSGEAVRAGATQVDAVAQVAEEGARRAEQTAEMTERASEEVAQ
                     ALAAVGAAQERTRLATERMEATAQSVAAIGDFVTTIRTIADQTNLLALNAAIEAARAG
                     EAGRGFSVVAEEVRKLAEQSNDAAKEVESRMEALSTSTAGALDAIRLGDQVMGDATVR
                     GNQARGVLGEALASSREIVRATREAAQAVIQHGKESQHIAQEIGHAEESTGETLTSLE
                     GIRVAIAETTRSSEQVAQESSRIAEEADHMRELLGRFHTEARALE"
     gene            complement(20136..20999)
                     /locus_tag="PL162_02680"
     CDS             complement(20136..20999)
                     /locus_tag="PL162_02680"
                     /inference="COORDINATES: protein motif:HMM:NF021982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HQF90970.1"
                     /translation="MGGASEGDASHRQVSWRWEARDRTATVHLPSGAPGPGLLPLVVF
                     LHGAGGTGVLGREQTGLGALADEEGFIVAFPDGTGARPDRLTWNAWYCCGYALDRRVD
                     DVGALDALLDLLERTFPVDPRRIYLAGFSNGAMMAYRYALERPGRVAALGVVAGALRG
                     GDRKAREALSVCIIHGTKDGVVPPDGKSFARPQRGRPPGEAVESAARFWAAANGNDPT
                     PHKDRRGQDTILRWTGGRMGAEVELHLIDGAGHAWPGGNPRKYRYCDEPPKSPEASRV
                     LWRFFSRHQRP"
     gene            21373..21807
                     /locus_tag="PL162_02685"
     CDS             21373..21807
                     /locus_tag="PL162_02685"
                     /inference="COORDINATES: protein motif:HMM:NF018037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1036 domain-containing protein"
                     /protein_id="HQF90971.1"
                     /translation="MNLRRRWGILLGAMVWGAALFGVAEAGVKVVLKNNSFENIVVAI
                     NYLAPNGRWITRGWWDVPSGKSVEPNVTSENTHFYFYAEGDNGGRWNGEDDAEGISRW
                     VVDEKFDHYDDQKGNPAGSNGRQVSFFHAEAKNGVVEQKFSD"
     gene            complement(21865..22422)
                     /locus_tag="PL162_02690"
     CDS             complement(21865..22422)
                     /locus_tag="PL162_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQF90972.1"
                     /translation="MLDDLSKHVLDIAENSMAAGARRIVVTLQERDREGWVELRVEDD
                     GGGMDPETVARVTDPFVTSRTTRRVGLGIPFLKQVAELCDGELHLASEKGKGTTLAAR
                     LRADAVDCPPLGDLAATVVTLLAGSPDRSWTFRFLRGEGVFEVDRDALVEILEDPRLF
                     QTPAVAHWVRNYVEEGLTELRRPEN"
     gene            complement(22476..24116)
                     /locus_tag="PL162_02695"
     CDS             complement(22476..24116)
                     /locus_tag="PL162_02695"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HQF90973.1"
                     /translation="MRYPHAKLRHLEERVAFLSEELYAAMEALDMALGLGAFPATIVT
                     LRDRDAVLRETEEKIRTMMPFKAAAFYLVDEGDASFSLVRCHPEEWRKFFERERPPLV
                     EDRTIAWALGRRRGVVVGATDGLEQILIHSLATPTGVMGFFLGVLGMKKHEIPDLAMD
                     FLSLTLLSCAGVLESFALNEHIHRLNAILQSHVASLQISEEELCRYRDQLEEQVRERT
                     GELARANDDLRQEIAERRRAESELREVLDRYDRLTANAHEAVFRMAFSPDQTSRVVYL
                     NPAAERMLGYSLAEWQKDPLLPEKVFDAESLKKVNSLIDRARWGEEGIPSEVIHWRTR
                     DGRPLIVDYSVLPEVDHQGKVLGCEVIARDITERMMLEARFRYQATHDALTGLPSRML
                     FSDRLALALAHARRNRSMVGLLFLDLDQFKHVNDTLGHDVGDLLLKAVAERLKGEVRE
                     MDTVARMGGDEFTVVLPDLEHPDAAGTVAERILEELKRPFVIQDKEIHIGASIGIALY
                     PAHGATVKELMKRADMAMYAAKGAGRGTFRFWQETPHP"
     gene            complement(24121..25416)
                     /locus_tag="PL162_02700"
     CDS             complement(24121..25416)
                     /locus_tag="PL162_02700"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HQF90974.1"
                     /translation="MGILSAGALRPGMVLAKDLRAPGGRLVLSKGATLSAKHIETIKT
                     WGIAGADIENSSPQDPRESLGERVPAACFEEGQRRVDRRFAAGNTAHPFLEELYRIAV
                     TREALRLARGELSDRSPASSGDPSSRRSPGKIPPADKLIAEEVSLASFPDTYFRIVEV
                     IGSPRSSAWHVASVVEKDTSLTAKLLKLVNSAFYSFPSRIESLPRAVALIGGNELSTI
                     ALGISAVSMFPDVPESFVSMKSFWRHSVATGVLARLFSIAHPEVFQERVFVAGILHDI
                     GRIVLLRRLPGAMTLAFERQRREGMPLHEAEEGVLGYDHARLGGLLLRRWQIPSAIEG
                     AVAWHHAPARAPQAMEPALLHLADLAAIALGEGIHPEQVVPPLDEEAWRLVDLAPESM
                     ETFVTQSERQIAEVLEMFGADGGDPREGLSPAPRSPEEV"
     gene            25506..26753
                     /locus_tag="PL162_02705"
     CDS             25506..26753
                     /locus_tag="PL162_02705"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HQF90975.1"
                     /translation="MNSRLELRCVLCGRSYPPDAATHTCASCGLDGTLDVLYDLDEVR
                     RSLSREGLARDFRPCLWRYAPLLPVEEEPFIPPLAVGWSPLYRSDLLARRYGIKAFYV
                     KDDGRNPTGSLKDRASAVGVARARELGQSAIACASTGNAASSLAGFSTVAGLPAYIFV
                     PESAPEGKVAQLLIYGATVVLVRGDYAAAFDLATAAMDRFGWYNRNCAINPFLVEGKK
                     TCALEMAEQLAWEVPDRVFIALGDGCCLSGLYKGFRDLKELGFIEKIPHLVGVQAEGA
                     APIVRALRDGASRVLPGTAVTLADSIAVGSPRNWAKALRALRESAGAGETVTDDEILG
                     AMRELARSTGVFAEPAGAAAFAGFCKAAREGRVAKDEVVAVVVTGNGLKDVPSARRAT
                     GAPLVVDPDLGDLLRKMAEGGAR"
     gene            26869..28344
                     /locus_tag="PL162_02710"
     CDS             26869..28344
                     /locus_tag="PL162_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HQF90976.1"
                     /translation="MIDLTIHPAPRRAAIRRARERGIVLPTFEEMKHPEKISPKIKER
                     LRHVGLWDLDSANLFRISWKNAPQPSGGLHGGVNYFEIPSALSGVRARIVGLVGKWFP
                     TGAHKVGASYACLVPRLVTGQFDPTAQKAVWPSTGNYCRGGAYNAALLGCESVAILPA
                     GMSPERFEWLSKVAGEVIATPGSESNVKEIFDKTWELRRTRDDVVIFNQFDEMGNHLW
                     HYEVTGGALEEVFQALRGDRGRVAGTFFTSGSAGTLAAGNAMKDRHPRSKFAVGEALQ
                     CPTLLKNGFGAHRIEGIGDKHVPWIHDVRETDLIVALDDEDCMALVRLFNDPAGRAYL
                     KGRGVAEEVVDRLDLLGISGVANLLGAIKMAKYYELSEDDFVFTVFTDSMELYGSRLE
                     EMAEVRGPYDAVQAAVDCHAHLWGIKDDGLIELTYPERQRVHNLKYYTWVEQQGRTLE
                     ELQAQWYDYDAYWGKLRRQAGAIDRLIEDFNRESGALEQLV"
     gene            28380..29600
                     /locus_tag="PL162_02715"
     CDS             28380..29600
                     /locus_tag="PL162_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HQF90977.1"
                     /translation="MAMTLDFAAIRQRAEHHRPKIVRFLRDLIALPSPSGGEERVVGR
                     IREEMEDLGYDRVEYDPLGNVLGYLGSGPRLVALDGHVDTVGVGNRDLWAFDPYEGFE
                     DEECVGGRGASDQKGGVASAVWAGKIVRDLGLLPPDVTLLVTGTVQEEDCDGLCWQYL
                     IREHGVRPEFVLSTEPTSCRIYRGHRGRMEIKLSVAGVSCHGSAPERGDNAIYKMAPI
                     LEELQTLHGRLADHPFLGKGSLTVSEIWSSSPSRCAVADGCGISIDRRLTLGETAEIA
                     LGEIRDLPSVRAARGAVEMYDYATPSWRGVTYPTEAYFPTWLIEEDHPVTRGVVRAHR
                     GLFGEPVVDKWTFSTNGVAIMGRFGIPCIGFGPGHEDQAHAPNERTWKDELVAAAALY
                     AAVPATLEDRTAGT"
     gene            29722..30708
                     /locus_tag="PL162_02720"
     CDS             29722..30708
                     /locus_tag="PL162_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HQF90978.1"
                     /translation="MQSIFRKKHFITLQEWTREEIDTLLEVSGDLKRRFALGEPTPLL
                     PYQTVFLMFFEQSTRTRNSMEAGITQLGGHAHYLDTSTMQLSHGESPKDTAIILSRFG
                     HAIACRNCFWKVGNAYLREMARWATVPILSLQDDLYHPMQALADLMTIQEKRGKNSER
                     LKVSIIWAFATSHKKPISVPLSQVLLFPRYGMDVTLAYPEGWDLPEWVIDEARKNAAR
                     HGGSVRLTHDQEEAYRDADVVFPKNWGSWVKNESTTVVDGALEAQRHWKCTERLMELA
                     SRDCLYMHALPADRGNEVEDAVIDGPHSVVYDEAENRLHTAKAVMALTMGGR"
     gene            30763..31575
                     /locus_tag="PL162_02725"
     CDS             30763..31575
                     /locus_tag="PL162_02725"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HQF90979.1"
                     /translation="MSLTWYVPREVQEVPELLAREGVYPHGGGTLILRGKGAKIRGLV
                     DLSLLGLDGVRFEQGTVALGAMVTFAGCVARLGRHDPDHILVKALSRAASTPLRNRIT
                     LGGSVAAAPIWSDLLGPLVALDAEVILRGPEGSTVTPVASFVREGASWRGALVTGLRF
                     REEGWRAFYHREVTVAFDSPAFTLSILARVSPGRVEELRVVLTGSRERFIRLVTMEEL
                     IQGKDPCGVKVEGLGERFEAAFPDRRHGSGAWLRTVAGVALERGLREVLGPC"
     gene            31569..32057
                     /locus_tag="PL162_02730"
     CDS             31569..32057
                     /locus_tag="PL162_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HQF90980.1"
                     /translation="MLNEEDRRITGTFSVNGRSRSWSFSPEATLLHVLRDHGHTEVKN
                     GCEEGECGACLVLLDGKPVPSCQVFAATARDREITTVRALGTIHAPHPIQEAFVEVGA
                     VQCGFCTPGIVLATAALLRENPNPSDEDIIHALDGQKCRCTGYVKIIEAVRLAARRGR
                     HE"
     gene            32050..34377
                     /locus_tag="PL162_02735"
     CDS             32050..34377
                     /locus_tag="PL162_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQF90981.1"
                     /translation="MSEFQVVGHSVTKYDALALATGEARFTDDFPHEGFLELALLWSS
                     HAHAEIVSLDTSEAEGVPGVVAIFSAANVPRVLYTSAGQGAPEPSPYDMVLFDTTVRH
                     VGDRVALVAAESLAAAREAVGKIRVEWKPLPPLFDAEAARDEGAPKLHGGDHHAPLPV
                     PYDPGHNLAASVSFDFGDREGAFDRATRVEEHEYRVQYASHCALEPHAALASFDERGR
                     LVLVSSTQVPFHARRITARVLQIPTGMVRVIKPRIGGGFGGKQEVLIEPLVALAAWRT
                     KRPCRLVLSREEVFVSGRTRHPVRLRLRTGADPRGNLTALDLDALMGAGAYGPHALTV
                     LSMVGGKTLPLFNKIPAMRFQGNSVYTNLPVGGAYRGYGATQGFFALNQQIDMHAREA
                     GEDVISWIKRQTIRAGETSPVFKALGEGKEGVDQIVRSCALEACLDRGAEAIGWKEKR
                     GRRLCPTPDTVRGVGVAVAMQGSAIPSIDLASVSLKMNEDGSFNLLAGAADLGTGADT
                     ALGQIVAEVLTVPTEKIVVLSGDTDVTPFDVGAYASSTTYLSGKAAEKAARDVASQIL
                     AVGARMLEVPREELTLAAGRVCHAPSGRSVSLEEVACDALYARDQHQIQASASHVAPE
                     SPPPFLAQFAEVEVDRRTGHLRVIKFVSAVDCGQPIHPALAEGQVEGAALNGIGYALW
                     EEYRFDGQGRLTNPNFWDYKIPGVRDLPEMVTILVPSSEPSGPFGAKSVSEIGINGPG
                     PAIANAFFDATGVRLFDQPFTPEKVLRALREAGGS"
     gene            34374..35729
                     /locus_tag="PL162_02740"
     CDS             34374..35729
                     /locus_tag="PL162_02740"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQF90982.1"
                     /translation="MILRGGLLALPGEEHGVVGDVRISQGRIVEIGEDLADDGAVMDA
                     RGLVVLPGGIDSHVHFNDPGYTSREDFYCGTCAAAAGGITTVVDMPCTSVPPGTSRVH
                     LMEKLAVVKRKAVVDFGFFGGVSGQSYQDAVGPGMAEMAPSVWGFKTYLTSGMKTFER
                     LNHWQFRSCLERAAEVRRPVLVHAEDFDYVQAATAACTRRGDSPRLFYQSRPETAENI
                     AVAAAVEMAEETGASLHVVHVGSARAAALVRDRAASGRITCETAPHYLNFDVEDFVAL
                     GAPLKVTPPVKAPENRASLWRFLAEGSIAFVASDHAPCQDKDKKTGSIWTDYSGIPGT
                     GTLMPYLFSEGYLAGRLSLGRLAEVTSGAAARRYGVAERKGALAPGRDADLALFDPGR
                     PGVVSGALFPSKGKITPFEGMTLRGTLVATFLRGEMVFHRDRGVVAPPGTGRFVTPAG
                     A"
     gene            35813..36328
                     /locus_tag="PL162_02745"
     CDS             35813..36328
                     /locus_tag="PL162_02745"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HQF90983.1"
                     /translation="MHFPSSPDLALRRTVEEDLDFVVAAENHPENRPYVFQWSRERHR
                     AALANGDLRHLVLDALPERRPVGYAILAGIQNAHRSVELMRIVVTEKRRGYGREGVRL
                     IQELAFDFLGAHRLWLDMRTYNDQARELYRSTGFVEEGILRDCVLVDGVFYSNVIMSM
                     LEGEYRRLSKK"
     gene            36400..37308
                     /locus_tag="PL162_02750"
     CDS             36400..37308
                     /locus_tag="PL162_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013355.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQF90984.1"
                     /translation="MAQRAVTVNGITFSNPVLPAAGPNVRSAALLQAAVDGGAGGIVT
                     KTVSVRPAVDPRPTLAKGSGGGMQNCETWSETPVEEMLEAYRAVKARGVPLVCSLGYH
                     PDELRTLGPLLEREVAPDALEFSVHYVGKSLDPLLEVARALRESVSLPLWMKLSPSIG
                     DVEGLARAASPFVDAFVAINSVGPALDFDPADCRPRLGSPWGQGWLSGPPILPLALAF
                     VFRVAQAQPKPVIGVGGIASGEDAAKFVAAGASLVQVCTAALRGGPGTYGRIDGELDA
                     WLDAQGYANLAELRGRYATALKGGRP"
     gene            37305..39593
                     /locus_tag="PL162_02755"
     CDS             37305..39593
                     /locus_tag="PL162_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HQF90985.1"
                     /translation="MTPTGNVLGSTPRRADAWEKVSGRAEYVDDLSIPGLWFGGTLRS
                     AVPRGRLRGISRRESFDWSRVVVVTASDLPGPNEVAMVRSDHPILAADAVNFVGQPLA
                     LVAAPDRQTLAAALGALVPDVEEAPAVLTLEEAQEGRTVIWGADNVLAAYVLGDEEDA
                     VAEAFSRADRIVEGTYTTGYQEHLYLEPQGMVALPREDGGVEVTGSLQCPYYVHRALQ
                     RALGLEDHRIAVRQAVTGGGFGGKEDYPSVLAIHAALLARASGRPVKMVLGRNEDLRF
                     STKRHPSVVRHRTGVRSDGTLCAAEVDVLFDGGAYTTMSPVVLSRGALHALGAYRVPR
                     ARVTARALATNTPPNGAFRGFGAPQTIFAVERHMDLIARELGISPLEVRRRNLLRRGD
                     RLPCGQILTEEPGASLVLERAQALAKYETRHGTLRKEGRGLGLSLFIHGGGFTGSGEE
                     KIDGRVAVEIGAGGRVEILASSTEMGQGAATVLPQIGAQALGLPLDRIFHPAPATDRV
                     PDSGPTVASRTVMVVGRILVGACEDLAAKVARRLEEAAGLPEGSVTWREGTFRGPGFP
                     EKSFEGVLDQWKDRLQGLRGEGRYAPFPDLHWDDDAYRGDAYKDYSWGANVVEVAVDP
                     DRHTLTLCAVTAVVEIGRAVHPLLAEGQVAGGTLQALGYGGMEDLRIDDRGRYLQDRL
                     STYSIPTALDAPDLVVEIAEIPSPRGPFGAKGLGELPINGGAPALASAVEDATGVFIS
                     TIPITGERLFAHRRDGLSDEPS"
     gene            39606..40958
                     /locus_tag="PL162_02760"
     CDS             39606..40958
                     /locus_tag="PL162_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HQF90986.1"
                     /translation="MILSFAVNGKTVTVEGPPLRRLLDVLREDLGLTGTKEGCGEGEC
                     GACAVLLDGHLVNSCLVPLFQVAGRSVETIEGMAPQGSPDALQRAFMDEGAVQCGFCT
                     PGMVMASRALLRENPSPRRKEIRTALAGNLCRCTGYEPIFRAVEGAAAAGYEVAPPPP
                     APEFRVTCTEEGRIAFFPESLPEALEILRQHPDAVPVAGATDLLPDLTRGGLPGPHRV
                     MDLTRLEELRGIREEDGVLVLGAGETFASLGRSPLVRRLFPSLGECAASVGGVAVQNR
                     ATLGGNVMNASAAADSPPVLLALDARGVLVSGEGTREVPAKELWTAYRRTARRPDELL
                     TAVKIPRPAPGTREAFFKLGPRRALVISRLTLVCAARLEEGRWRGVRIAVGSVAPVPH
                     RLEELETFLEGRVPEEGAVREARRLAEDLLRPRTSREYRRDVGGNLVGRFLSSWISPR
                     "
     gene            41117..42040
                     /locus_tag="PL162_02765"
     CDS             41117..42040
                     /locus_tag="PL162_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90987.1"
                     /translation="MAGRTKNSKYYALKGVVNVRREEADPLQAFFYALFDPGNLHRFS
                     LRLMSSPSMNIKRPWDEYGIMLEEAEEDRENAETEGTIRTVVHQVVEGIKFAEISGDN
                     PIALQKLETHLNRSVGSALSGTVNAFVKVESCSTDAFHSFVAKFNPPAEGSSPPSSPA
                     GSPEEGAGEGAGGSLLDREIVLVCQPLVDPLRGKAATAVLPGDVILVKIPEDSIFFSM
                     LTKIKKGFFDGVVEAVVTRVDRHSAETVVMETALAENVKGKVPAPNALRVKLSPSTLQ
                     EGGKPPSAPLVWALVGGGALMLGVLLWYLMT"
     gene            42196..43863
                     /locus_tag="PL162_02770"
     CDS             42196..43863
                     /locus_tag="PL162_02770"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HQF90988.1"
                     /translation="MASDLEIAQKVSLKPIMEIGADLGIPAEAIIPYGHYKAKVDLRF
                     LKTLKDRPEGRLILVTATTPTAAGEGKTTNTIGLTQALVKMGKKAMICLREPSLGPCF
                     GVKGGAAGGGWSQVLPMEDINLHFTGDIHAVGTAHNLLAAMLDNHLQQGNALGIDPRT
                     ITLRRAMDMNERALRHTVIGLGGKAHGVPRETGFDITVASEVMAILCLAEDLMDLKER
                     LGRIVVGLNFKGEYVTAADLKAHGAMASLLKEAINPNLVQTIEHVPAFVHGGPFANIA
                     HGTNSLVATRAALKLADYAVVEAGFASDLGAEKFMDIACLYGRFSPSAVVLVTTVRAL
                     KLHGGAPKDQLGTPDPDALRRGLPNLEAHLDNLNNFGVPVVVALNRFSSDSDEELALV
                     REAAASRDARIALSEVWEKGGEGGLDLAREVLAAMDQGSAYRRLYGEHLPLKDKIGTI
                     VTRVYGAEGVDFIPSAAAKLEELEQRGYGTLPVCMAKTQMSLTDDASKKGRPRGFRVT
                     VREVRLSAGAGFVVAICGDIMTMPGLPKSPAAERIDIDSEGNITGLF"
     gene            complement(43972..44343)
                     /locus_tag="PL162_02775"
     CDS             complement(43972..44343)
                     /locus_tag="PL162_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF90989.1"
                     /translation="MEMITCDLCNKPFASTGPRVCVRCMKRLDTVYAKARDFIRDNPK
                     ARLDVKEMAEALEVDVRDLEVLIAQKRLDRDADNAMGEVEGEESRRKKLLESFQRSAS
                     ELSGEPKKTYRSYGEERYGKR"
BASE COUNT         7518 a        14640 c        15393 g         7274 t
ORIGIN      
        1 ggcgaaagag agaggggggg atgaggaagg atcgcagggc gaagggatcg gaaaaaagac
       61 acgacttggg ccctccgggg ggtccctaga caggggccgc accctccgca gggaacggcg
      121 ggggggcggt ctccgaagta gctcaggaga cgctcccgga ggcatcccga ggcgcggcag
      181 tacgacgtca tgtcctggag cttcttgaga tcccggcgca ccccttcccg atccctcccc
      241 tggcagacga ggaatcgggc gatctggacg tctttggcgt cgaaaagcag ggggcactcg
      301 gcgggctccc cgtcccgtcc cgcccggccc gcctcctggt agtagctttc gaggcttttg
      361 gtcatgtggt agtggaggac gtatcgcacg tcggacttgt cgatgcccat gccgaaggcg
      421 ttggtggcca ccaccacgag ggcccggtcg tggaggaagt cctcttggtt cttgacgcgc
      481 tcccggtcct ccagccccgc gtggtaccgc gtggcggcga tgccgctgcg gcgcagcaga
      541 tcccaggtcg agtccacggc cctgcgggtg gcgcagtaga cgatgccgga tttccggggg
      601 cgtgccgcca cgaaggaccg gagaaaggcc tccttgtcct ccgggcgctc cacccggagg
      661 aggaggttgg ggcggtcgaa gctcgtggtg agggagaagg ggctccgcag gtcgagcttt
      721 ttctcgatgt cttttcggac ctcgggggtg gcggtggcgg tgaaggcccc caccggggga
      781 cgtcggggca gggccttcag ggcggaggcg atggccaggt aggcggggcg aaagtcgtgc
      841 ccccactggg agacgcagtg ggcctcgtcc accacgaaga actccacagg gagcgtggcg
      901 aggagcttta ggaaggtcgc gctctccagg cgttccgggg cgacgtagat cagggggcac
      961 tccccggagc gcagcgccag gaccacctct cgaaactccc cctcccccag ggagctgtgg
     1021 agggcctggg cggcgacgcc gctttgcctc agggcgtcca cctggtcttt catcagggaa
     1081 atgaggggag aaaagaccac ggtgaccccc ggccggagga gggcgggcac ctgataacag
     1141 accgattttc ccgcccccgt gggcatgacg cccagcacgt cttgcccctc cagcagacgc
     1201 ttcaccagtt cctcctgtcc cgggcgaaag gcgcggtggc cgaagacccg gtgaagcacc
     1261 tcgagggggg tgcgccccca gggccccccg gaggccctag ggctcatccg agatcccggt
     1321 gaggaggcgg acgtagccct cgagctcccg ggcccgggcc cgggtgatct gcatcacccg
     1381 ctcggcggtg aggactcccc agatcgtccc cccctcgttg acctcgtaga gctcatttcc
     1441 cagggcttgg gccgccttct cctccgcgtc gcggtacttg atccagaggc gttgcgacgc
     1501 cttcaggacc tccatggcct cgggacgaag cttctgggcc aggaggcggt attgggcgtt
     1561 tagctccccg tcccagcgct tcagcccctc ggcgtaggtc tctcgcatcc ccgccgtgga
     1621 gtcgtcggcg gccagggcct cctcgaccca ctggtcgatg gggtgggtcc tctcttcggc
     1681 ccccgccggg gggatccaga caaaaaggga taaaatcatc gtcagcgcca aagcagtcag
     1741 gttttttccg atggtcatgt cgttcctccc cgggttcttc cggcaaaaga tttcttccgg
     1801 aggatcataa cacaagaggg ataggtgggg tcaactaaga cagagcaagg ctttgataaa
     1861 aagagatttt tccgcaaaaa atggcatttt ccaaccgcca tcggaaaaag atccccgcag
     1921 gcccccatgg ggtctttcct ccgggggtag tatactcgta aaggtcagga aagatcaaat
     1981 gtgagcccgg gggtcaaaag aggggggaaa gaccatggcg gtgcgataca aggactacta
     2041 tgcggtgcta ggggtggatc ggaaggccac ggcggaggag atccaaaagg cctttcgggg
     2101 ggcggcccgc aggcaccatc ccgacgtgag caaggctccg ggggccgagg agcgttacaa
     2161 agagatcaac gaggcctacg aggtgctgaa agaccccgaa aagcgcgccc ggtacgacgc
     2221 cttgggggaa aactggcagg aggggcagga ctttgccccg cccccgcccc ggcggggagg
     2281 aaagggcttc ggctcctacg gggggcagga gatgcccttc ggggatttca gcgacttctt
     2341 ccacgtgatc ttcggcgggg gcttcggggt tccgggggat ttcggggcct tctccggggc
     2401 ccgggcgcga cccgggggcc cccggcgagg ggaggacgtg gagttctccc tggagatctc
     2461 cctggaggag gcccagaggg gatgtcgacg ctccctcgtg tgggaggatg acggggggaa
     2521 gcggcagacc ctggaggtgc gcatcccctc gggggtgacg gagggctccc gggtccgggt
     2581 ggcgggcaag gggcaccccg gatcgggggg aggggccccg ggggatctct acctgcgcat
     2641 atccctcctg ccccacgggc gcttcgccgt ggacggccac gacctgcgca cctcggtgga
     2701 ggtggccccc tgggaggccg ccctgggggg ggagattccg gtggagacga tcacggggcc
     2761 ggtggagatg aaaattcccc ccggcaccca gggaacccag gtctttcgcc tgcggggcaa
     2821 ggggctcccc aagaggcgaa aggacgaccg gggagatctt tacgtcacgg tccgcatcgc
     2881 catcccgcgc caactgagtt ctcgggagag ggagctcttc gaggagctgg cccgggtctc
     2941 gacttggaag ccccgggggg agtagctggg cccggtcagg ggagggtcag gtcggagagg
     3001 tcctcttcct cttccggggt tggccgggcg agccgggggg gaggggcgtc ccgcagggcg
     3061 gccacccagg gcaccgtccc ccggtcgatg acggccagac aggcgtccac caccccggga
     3121 tcgtacttcg tcccccggtg gcgcgaaatc tcctcaaggg cggcggccat gcccagggag
     3181 gaacggtagg ggcggtgggc gttcatggct tccaccacgt cggccaccgc gatgatgcgg
     3241 gcctcgggga ggatctcctc cccccggagc ccctggggat accccgaacc gtccatccgt
     3301 tcgtggtgct ggagcaccat ggctcccaag ggccagggga gctcgatgcg cttcaggatc
     3361 tcctgcccct gggaggggtg gagcttcacc agggcgtact cgtcctcgct gaggaccccg
     3421 ggtttacaga gcacctccga ggggacggtg atggtcccca ggtcgtgcac cagggcggcc
     3481 atgcggactc cgtggacgcg ctccggggaa aagtgcatga gggaggcgat ggcggcggcg
     3541 agctccgcca cccggctctg gtgtcccacg gtgtagatgt cgtgggcctc cgaggtccgg
     3601 gagaggacct ccagggtctg atcccaactc ttctggagct gggccacgtt ccgggcgagc
     3661 tccctctggc tggcggccag ggcctcggcg tggtggcgat gttccagggc gttggccacc
     3721 tgggtcgcca tgaagttcag gatctccatg gcccggggag ggtaccggac cccctcgtcg
     3781 taggtctgca ccgccacgat gccgaagacc tccccgttgc cggcgcgcaa aggggccccg
     3841 agccactcgg aggggatggt tccccggagt tggcccctct ctttcatggc catgcaggcg
     3901 gcgtggtcgt agaggattgc cgtgcccttt tggatcacgc gaaaggaaag ccccaggggt
     3961 tggaggggga cgccgtgcca ctcttcctcg gagaggtcgt ccttttcgtc cacgtggtag
     4021 acgaagcgga cggtggtctg ctcccggtcg cagagggcga tgtaaaagtt ctttgcgggc
     4081 atgagatcgg cgaggattcg gtggacatcg gcatagaggg cctcgagggg agcctccccc
     4141 cgggcggtct cggagatgtg gtagagggcg gcttggatcc tctcggctcg cttgtattcg
     4201 gtgatgtcgc gctcgaagac caggatcccc gccacggccc cctcctcgtc ccggaagggg
     4261 atcttgtccg ccagggtcca gaggagctcc cccgaagggg tctcgtagcg ctgcaagagg
     4321 ccatacaagg ggcttcccgt gcggagcacc tcccggtctt ggtcgaaaaa ccgagccccc
     4381 tcctggggga agagctcttc catggagagc ccctcggccg cagtcgacac cttgccggtg
     4441 aggcgctcga cgtaggggtt ggcccggagc atgtggttgt gtcggtcctt cagggcgatg
     4501 gcaaaggggg cctcttcgaa gatgaggcga aagccctttt gttgaaaaag atccaacaat
     4561 cgctgccggt cccggtcgtg atccgatcca cactccgcca cgtccccatc cccctttcgc
     4621 ccattggagg ggcctttctc tcattctaag gggcggaggg gctaagggca agggcgtctt
     4681 tgatattttt tatatttctc ggcccaagga tctccggggc aacgcctctc ccttttcccc
     4741 cggggccttt tcccgtataa tgcccgcacc tcaaagtctt tcttccaagg ctttggggag
     4801 ggctccgggg aaagccccgg gcccggagag ggggtgggcg cgatggccat gatgctggcg
     4861 gtggaagacc tggtcccggg ggtggtgctc catcggaacg tgacggaccc ctcggggcgg
     4921 ctgctcctgg cggcgggggt ggcgctcacc gaggctcaca tccggagcat ggaaaagtgg
     4981 ggcgtggtgg cggtgcaggt gtgcttcggc gccgccgacg aggaccggac ggagacgggt
     5041 cccatccccc ccgagctggt gcaggagacc aagagacaac tccgggagat cgccggattt
     5101 ctggagaaca aaaaaagcat tccccgggcc tcctttcacc tcctgacttc gactctcaac
     5161 agcgtgatcc aagcgctcct ggatcaccgg ggggagctct tcgaagaggt gcgcctcata
     5221 tcccaccacg acgcccatac ctacgatcac tcctggtccg tctgcgtcct gtccctggcg
     5281 ctggcccggg aggccccttc ggtgggtctt tcgggggcgc cggactactt gtcgcgcttg
     5341 agcctggggt tgggaggggt ctttcacgac ctggggaaga tcttcatccc cctggaggtg
     5401 ctcaacaagc ccggggccct cacggcggag gagttttccc tcatccggga gcacccccag
     5461 cgggggatga acgccctgcg gacctatgag agcgtccccc ccatggcccg gagcatcgtg
     5521 ctacaccacc accagcgatg ggacggcaag ggctacggcc cggctagcgg ctttctcctg
     5581 gagggagaag agatccccca cctggtgcgc ctggtgtcgg tggcggacgc ctacgacgcc
     5641 ctggtgtccg accgccccta ccgtccgggg tacctccccg gagacgccct caccgtgttg
     5701 cgccaggccg ggggatcgta cttcgatccc cgggtggtcc agctcctctc cggcgcggtg
     5761 cccgactatc cccggggggc ggtggtgctc tccaagggag ggtgggtggt gcacgtcgac
     5821 cgggcggtgc gtcggggaga ccccttgcgg ggcaccgtgg tggggtcctt ggcggccgaa
     5881 ggccaatcgg cggtggggga agaggtgacg ctcacttcgg ccggtctcct gtgcggcggc
     5941 acctccgtgg ctcacctggc ccggaggctg gagtccctct ggcacctcca cccctccctg
     6001 gaggaacacc ccctggccct gcactccttc cccccttggg acacgctcct gagacaccac
     6061 ttccgccggg gaatccccgt gatgccctga cgcccccggg gcccaccttc cgggcggcgg
     6121 gatcttccat aaatctttac cgccggagca gacgggagtc ccctgcctcg aagcagggga
     6181 tgcacacgct ccgccccccc aggaggcgcc ctcggctttc catgaagggt tctccgcaaa
     6241 gggggcagga gagggtctcc cggatcgggg cctccggggg aaggataaag ggcacctcgg
     6301 tccgctggaa gaggtcgtcc aggcgggccc ccaggatctc gttgcaacgc cggagccgcc
     6361 gcgcctccgg cgaggccccg ccgtccgaca aatcccggcg ctggggccgg aggaaaaacc
     6421 gtagggcctt gccgtcctct cggcgaaaga agctgaaggc caccttgccg tggtccacga
     6481 agatcagatt gcccttgccg aaggtgcatc cggtgaggac ctgcactgcg tccaccgcgc
     6541 acatgtccgt ctccgtcacc gccaccacgt cctcgtcgcc gctccttccc acttcctcca
     6601 gggccagctc ccccgcccgc acccccaggg ccagccccgg acaccagtgg ccgtgaaagg
     6661 ccaccgccgc ccgaatctgg tcctcgctca accgacatcc cgtcatgttt ttctcctctc
     6721 ggaggcgccc cgggcgcctc ggcgatccgg gccccgccac atcgggggcc gcttccggtt
     6781 cctgacctcg acattcctga aagcgcgttt agggatcctc ccgggttttc ccaggagcac
     6841 tataatgggg gcgccaaggc tcccattctg aggacaacgg aggcgaagat catgactcta
     6901 cgccggaaaa tatttttctt ccttgccgcc gccctgcttc ccgtggcgac ggtgctcttt
     6961 ttctcggcgc gctatttcgt gctggacggc tttctccaga tggaaaagag ctccatggaa
     7021 caaaagctca agtgggcccg gggggccttc gactatgaga agaccaacct cgccgatctt
     7081 ctggaaagtt gggccgtgtg ggacgcgagc tacaactacc tgctgagccc cgacgagctc
     7141 tacgaagaag agaacctcaa cgaggaaaat ttcaagatca tgcgcctgga cgccatcgcc
     7201 tacgtgcgct ccgacggcgg cgtggtgacc gccctggggt gccatcggca cagcggcgag
     7261 aagggcccct ttcctgcggg cctcgaggcc cagctgactt cctccagccc cctggtgtcc
     7321 ctttcgtcgg acaccaagat ggtggggatt ttgtcccttc ccgggggccc catgctggtg
     7381 ggggccatgc ccatcctcac caacctgcgc caggggcccg tccacggtgt gctcgtgatg
     7441 ggacgttttc tcgacagcga catggcggcc gacctggcct agaaggccga tcttcccttt
     7501 tcggtggagc ccttcgaggc aagcgaggcc aagaacctct tggggtcgga aaggcccctg
     7561 gtcgtcgtgc cccgggacga ggatcgggtc acggggttca ccctcctgcg cgacatcgcg
     7621 gggcgccccg gcgtcgtctt ccgcgtcgat caggaacggc gggagctcca ggcggcccac
     7681 cggagcatcc tgatcctcgg agccattttc ctggcggcgg cggtcttcct tctgggggcc
     7741 gtgggcctgc cgttgcagcg gttcattttg cgccctctgg gccacatggt ttccaccgcc
     7801 cagggcatcg cggcgggaga cctcacccgg cgtttcgccc tggaggggaa agacgaggtg
     7861 gcccaggccg caggggccat cgaagccatg acggaaaaac tctgcgacgc catgcggcgg
     7921 atctccgaaa ccgccgcgcg cctcgacgac ttcgcccgga acttcgccgc tctggcggac
     7981 gagtcggagg cgggggccga gaagtcccgc ctccgcgtcg aggaggtggg ggccgaaatg
     8041 gaggggctcg ccagcacggc ggcggagatc gacgcgtcgg tccacgaggt ggcggaaagc
     8101 gcccagcgag tggcccagca gagcgccgac atggcggatc aggtggtagg ggcccaggaa
     8161 acgggggatg cgggcatcgc cgcccttcgg ctcgcggtgg cgggggtgga ggaaatggcc
     8221 cgggacgcca ccaccgccgc cgcatcggtg aagaccctgg gggatcgggc ccggcagatc
     8281 cagggctttg tctcccagat cggcggcatc gccgatcaga cgaacctgct ggccttgaac
     8341 gccgccatcg aagccgcccg ggccggcgag gcggggcgag gctttgccgt ggtggcggaa
     8401 gaagtgcgca agctcgccga agagtcgaac accgccgccc ggagcatcgc cgaactcgcc
     8461 acggccatcg cccaggacct cggtgccgtg ctttccgcca cggagcgcaa cgccgccgcc
     8521 gcgaaagagt cccaagataa ggtgtccggc acggaggggc gcatcgccgc catgatcgaa
     8581 gggctccggg ccatcgccgc ttccacccag gatctggccg ccgtttcggg ggagcaggcc
     8641 gcctccagcc aggaaatcgc cgcctcggtg caggacatcg gtcggaaagt cgccggaggc
     8701 acggtttcga cggcggaggc ccggaaagcc atgggcagcg aggtcgaaac cgctcggaaa
     8761 gtggcggaaa acgcccacca tctggcccag ttggccgacg agctccaggg caccctggcg
     8821 gaattcgtct ttgacgacga agaaggggcg tcgtctgcct cccccgccct gcccttcaac
     8881 cgggggcggt aacggcagcc gaaaaaatac ttctttcccg gagggggctc ctttttccga
     8941 aaaggggctc ccctccacag tgtgctcccc ggaagcagag gggcaggagg tccgggcctt
     9001 tacccccgag tttgcacgtc ccgggccggt gttccggaaa aagggtcttc gagaaagaag
     9061 agaaagtcgc agcggggggc cccttccatg atggtgctcc ggcgcttcag acggaggcgg
     9121 gggtccagcc cccgggcgaa ggcctcgtcc cgggcgcagg agagacaaaa gccctgctcc
     9181 cgggcgccga gcccttcgta gagttccgcg taggcgcagc ggagcacgtc gaaccggagc
     9241 ctggtgggag tttcctcaaa gaaggcgagg tccagggccc cttcggcggc ccattcttcc
     9301 cgcaccaccc gggccaacac ctccagcggg gccccttccc gccggttcct gcgggcggcg
     9361 ccgctggccc gggcgtcctc ggcgatcccc gcggaagcca cttcccgggc cttttccgcc
     9421 cccaggtgct tctcgaaggc ccgcaggagg gccagggctc cgggggcctg aattttccgg
     9481 cgctggagat gggacagggg gcaggatccc tccggttccc tgtcggaggg gagcgaagga
     9541 tgcccgagga gagggaacct ttccgggggc agctcaaggt cccaacgctc ctccaccagg
     9601 tcgcgccccc agaggtggtg cgtcacggat tccaccggat ggaacccgtg gcgggtgtag
     9661 agcttgcgcg cctcctccag ggtggccacg gtccacagaa agatcttgcc gtacccctgc
     9721 tcccgggcga actcaagggc gcggcgcagc aacgctcctc cccacccctg gccccggaac
     9781 tccggctgca ccaggaacca ccggagctgc acttcctccg cgctgtgccg caccatgccg
     9841 atggagcccg cgagggtgtg gccgtccgtc accacccaga cctcgctccc cccccggtgc
     9901 cgttcctcca gataggtcac catgccccgg aggacgtagg cctcgaagtc ctctccgaac
     9961 ccatgctccc gggcatagat ccgggcgtgg tcccgggcca ccccgaggag ctcccccttt
    10021 tccgccggac gcagcaccag agagccatcg tgttcgaagg ggaaattttc cgtgccgtgc
    10081 atggaaatcc ccctttcgtt tttcgtcatg ccgatttccc ggggcgaaga gatcccttcc
    10141 gggggtcacg ccagaacagg gtccctccca gggtgagagc cgcgcccccc agggtcatgg
    10201 gcaccgtggc tcccagggcc agcaaccccg cccccaggaa aaccccccgc tgcatcacgt
    10261 tgatcggacg gttggtgtac cccatcatgg cgtagccgaa ggcgaagatc gccgccacgc
    10321 ctcctcccat ggaaaccaga ttgcccacga gatccttctg ccagagaagc cccggatcgt
    10381 agcagaaggc gaagggcacc acgaaggcca gaactccgag cctcatggcg ataaaccccg
    10441 tggtgttggg gttggacccc gccagcgagc tcgccgcata ggccgccagg gccaccggag
    10501 gggtgatgtt ggagatgatg gcgaagtaga acacgaacat gtgggccgcc agcggggcga
    10561 agccgagctt ggcgaggaga ggcaccccca gggcggcggc gaggatgtag gcccccgtgg
    10621 tgggaagccc catgcccagg atcagggaga ccaccatgat gagcgtgagg gcgagaaagg
    10681 gcacgtcccg ggcgaaggaa aagatgaacc ccacgaactt gaagccgaac cctgtgaggg
    10741 acacggatcc caccacgatc cccgcggcgg cgcaggcgat gcagatcagg gggatgtttt
    10801 ttgcgcccat gtagatggcg tcgaagatgt cccggaggcc catgcggtgc cggggatcga
    10861 agagcgagac cccccaggcg agggccaccc ccaccaccgc tgccagcatg ggagtgtatc
    10921 ccgccagaag cagatagacc atgcccacga tgggaatcac cagatagagc ttgcgacaca
    10981 cgacccgcag atcggggagc tccgaggagg gcatgccccg caagccgtgc ttcagggccc
    11041 ccaggtgcac catgaggagc accgccccgt agtacatgag ggcgggaaag atggctgcga
    11101 taatgatgtt cttgtaggga acgcccagga gggacgccat gacgaaggcc cccgccccca
    11161 tcaccggggg catgatctgc cctccggaac tcgccaccgc ctccaccgcc ccggcgaagg
    11221 ccggaggata gccgatgcgt ttcatgaggg gaatggtaaa ggtccccgtg ccgtagacgt
    11281 tggccaccgc cgatccggag atggagccga agagacacga actcaccacg gcgatcttgg
    11341 cgggtccccc cgcctgggtc cccgcgaggg cctgggcgag ctccatgaac cagctcccga
    11401 tgccgctttt ttccagaaag cccccgaaga tgagaaaaat catgacatag gccgccgaaa
    11461 cgcccagggg aatggaaaaa atcccctcgt tggtgaggta gatttcctcg atgacctccc
    11521 ggatcggaaa ggaaagcccc ttcatgatcc ccggcatgta gtggccgaag tacatgtacc
    11581 ccgcaaagaa tgcggcgatg agcgtcaggg gccatcccac cacccggcgg gtggcttcaa
    11641 gaagcagcac cagcaacagt cctcccatga ggagctggac gtccgtcacg gggtccacct
    11701 ggacgatgcg ggaggcgatt tcgccgtagt tgagggcgcc atagatcccc ggcaagaggg
    11761 agatggcggc gagggcccag tcgtaccagg ggatgcctcc cactttcgcc gaagaagcgg
    11821 ggggagcggg ggcctcgcag gtcggagctt ccagggtctc tcccgccacg gtgcaggggg
    11881 aaagacgccg ggggtagagc agaaaggtca ggggaagcac ccaggccagg tggagggtgc
    11941 gctgcagata ggcctcgaag ttgccgaaga cggcggtgta gaggtgaaag agccccatgg
    12001 ccaccacata gacgtacaca aaccgggcgg aaaagccccg cagttctctc atagatcaaa
    12061 cctccccatc tcgtggatcg gtggcgccgg agttcccgca ctggggaagg ccgtggtgcg
    12121 ccatacgaac gcggggaggg caacggcatc cgttcccctc cccgcatctt ttcggaagcg
    12181 cccgccttag cgcacgccgg cccagtacgc cgccgccgcc ggatgggagg ccactttttc
    12241 cggcaccgcc tcccccggag tgagctcctt catgtccccc acggcctttt ccagatttcc
    12301 cttatcttcc cacatggtcc ggcagagcgc cgtcaccaga tcgtccggca ggtccttgcg
    12361 ccctacgatg caggtgtagt cccccaccac gggaacgggc tccgtgacgc acttgtagac
    12421 cccgggctcc acggtgaagg tcaccgtgcc gtaggcgtcg gcgagggcat tccgcgccgc
    12481 ctcgtcgatg gggagaatca ccacgtccac ctgctgttcg atctgcatca ccgtggaggc
    12541 cgtgcccccc acggaaaagg cgaaacagtc cagatggtta tccgccagca gattgccccc
    12601 gtcctcgtag gaggagaact ccaccgaccc cccccaggcc ttgaggtcgt cgtaggagac
    12661 cccgtacccc ttggcgaaga gggccttcac cacgaactcc gacgccgtgc cgggcttgag
    12721 ggtggcgaag cgcaggggca gcttcttggc caccacgtct cccacgctct tgaccccatt
    12781 cttttcggcg tagtccttgc gcatgatgaa ataggtgtac tggcggtaga ggtttgccaa
    12841 caccaccgcg ttgggggtgg ccttcttgaa gggctcctct cccttgaggg ccgcccccac
    12901 cagggaggtc accgaaaagc ccaggtcccc cttgccgttg tcggtgttga tgacgttgga
    12961 cacgcccccc cccgtgctgc tggtggtctg gagcgtcccc ttggtccaca gatcggccag
    13021 ggctcctccc agggcgaacc agttcccccc cggaggcccc gccatgacgc ggagctgatc
    13081 cggccatccc tcctgggccc cccccgcgcc ggccatgccc accacgagca tgatcccggc
    13141 gagaagaatt ccgagagctt ttttcataga catcctctcc ttctcgaaaa aattttgccg
    13201 agtccggcct ctgggccgtt ccgccgcttg acgaacatca tgaatcctcc ggagagctct
    13261 gtcaagaacg ctccaccgga ggccgataaa gggggggaga gtcgttttcc ccgcatcttt
    13321 tggaccgctc cgggtgcggc gctctgccga gaagagcccc gagggcatgg cgttctttga
    13381 gaaaatgatc tttgtaaggt ttgtaaaaaa acactccatt tgctagaatg tccaagtaaa
    13441 gcgacagaat aatgagaggg gattttccct tggcgaagcg ccccccgggg gcgcttcgga
    13501 gagttcttga caggcgacgg aggcggaaat tttccctccg tcgtcgggtt ttttcgagag
    13561 ctcccggaag ttttcagacg aaaaagaatg cttggtggag aaaaagcttg aagggagatg
    13621 atcggagcgg tggcgtggag aagttgtctc agccgggggt tctggaacct ccagcaaaaa
    13681 aatattaaaa aatagagaga ggaaggtttt ttatgtccac actgcgaggt cggttgatcg
    13741 gggtttttct gacggtggtg ctggtggctt tggggggctt gggatacacc gcgtttaccc
    13801 tgtcccgaga ggccctcatg gatctcggga agaaagaagg ggtggccctg gcgaagggcg
    13861 tggcggtgga gaccaacttc tacgtggagg gggtcgccaa cctcctgaaa agcgtggcct
    13921 ccacgccggc gctccagagc atgaactggg agctccagcg ccccgcgctg gagagcgctc
    13981 tgaagggaac ctatgccttt gagaccattt tcgtgctgaa cctctcgggc gaggcccgca
    14041 ccctggacgg ggtgacggcg aactacggcg accgggagta cttcctcgcc gtgcgggaca
    14101 cgggaaaact cttcgtgtcg aaccccctgg tttcccgggc cacgggaaac aacgtggtgg
    14161 tcttcgccgt gcccatcttc ggagaaggaa acgccctggt gggggtgctg gcgtcctccg
    14221 tcaacgccga gaccctcatg gcgctctaca agtccgtggt ctggggcaag acggggtacg
    14281 ccttcctggt ggggcgcaac ggcattgtcg cggcccatcc caatcaggag atcgtgggga
    14341 aactcaacgc gtccgaggtg gggccctcca ttcccgaagc cctcgccggc ggggtgcggg
    14401 gggctctggc gggcaaaagc agcgtggtgc cttacgagtt ccaggggacg gatcgcatgg
    14461 cggccttcga gcccgtcacc gccacggggt ggggtgtggg actttcctcg ccgaacgaag
    14521 agttcctcgg cgccgtcaac gagctccgca ccattctctt tctcgtcatc ggggtcattg
    14581 tgctggtggt ggtggccctc tccctctggc tcgcggggag catctcccgc cccgtggccg
    14641 ccatcgccca ccacatggaa ggggtggctt cgggggatct ttcccgccgg atcaccctgt
    14701 cgtcgtccct ccgggagatc cggaatcttg tgacctctct caatgaaatg atcgccgccc
    14761 aggggcgcac cgtggcctcc atccgggact cttcccggga ggtgctggag aaggccgaga
    14821 gcatgagcgc cgccgtggag gaatcccggg ccgccattct tgaagtggcc tccatgacct
    14881 cccgggtgag cgaccgcacc caggatgccg ccgccgccat cgaagaggcc aacgcgggca
    14941 tcgaggaagt gtcggcgggg gcccaggcgg gagctcatgc ggcggtggcg gcgggagaag
    15001 aggcggcggc catcgccacc gtggcgaggt ccggcggaga agccatggac gccatgacgg
    15061 gggagatcgg tcaggtggcc gcgtcccaga cccgggcgga tgacgccatg cagcagctgg
    15121 gcaaggccgt gacccagatc ggcagtttcg tcaccaccat tacccagatc gccgaccaga
    15181 ccaacctgct ggcgctgaac gccgccatcg aggcggcccg ggccggcgaa gcgggacggg
    15241 gatttgccgt ggtggcggag gaggtgcgca aactcgccga agagtcgaac cgggcggccc
    15301 acaacgtggg taccctcatc aacgacgtct cgtcccgcac ccaggcggcc ctcaccgaca
    15361 acaaagaggc ctccacccaa ctcagcgccc tggtgaaccg ctccaaggaa acccggcagc
    15421 tcattctgga gatggtgaag aaggtgggcc acatcaccga aagcgtccag tccatcgccg
    15481 ccaccatgga ggagcagtcc gccgccgccg aggagatggc gtcggggatg gaccaggtgg
    15541 ccaagtccag ccaggagatc tccgaggcgg tgcggaccat ttcgcactcc atggaagaac
    15601 aggacgccgc catggagatc atcgcggtgg cttccgaggg catggtgtcc ctgagccagg
    15661 ccctggagtc cgctgtggaa gtgtttaaac tcgacgagtc ctccggtgtg acgccccacc
    15721 ccgtggcggc ccttcccggg gcgccacgga aaatcccctc ctcgggggcg aaggaacggg
    15781 gcgcttccac gccgccgaag cggcggtagg cctccggttc cccgtgctct gggccttccg
    15841 gaaaagaccg aactcctcgg tcttttccgg aaggcttttt cgtgtccggc gaagctcttc
    15901 cggaggaaac aggtctgaag gtctgccctt acctcgggag ccttcgccac ctccggaagc
    15961 cccgcccctc tcttttagcc ccctgttcgg tccccctctc cgccttccgt tccttttgtg
    16021 taaaataagg ccttggggga gggggcgcgt ttttacccca cccggggggg gatctttccc
    16081 cgacctctga aggcgggata tggcgcaaag aggtcttttc ccattcccct tgatcgaaag
    16141 gagaatcttt atgaaagaat ttgccgccgg aggttggacc cggaagactc tggccgccac
    16201 catcgatcac accctgcttc gccccgaggc gtccgaggcg gcggtgcgcc gcctctgcga
    16261 ggaggcccgg acctacggct ttgcctcggt gtgcgtggcc ccctgccacg tgtccctggc
    16321 ggtgtcgtgc ctggggggac ccgagggggg cgtcgtcggc actgtggtgg gctttcccct
    16381 ggggtatcag gatcccgtca tcaaggccgc cgaggcccgc cgagcccggg atctcggggc
    16441 ccgggagatc gacatggtgt tgaacgtggg gtggctcaag gagggggccc gggatgcggt
    16501 ggcatcggac atccggggtg tggtggaggc ggcgcgggga ggggtggtga aggtgatcct
    16561 ggagacctgc ctgctcaccg acgaggaaaa ggtggccgcc tgcgccctgg cggagcgggg
    16621 gggcgcccgg ttcgtcaaga cctccacggg attcggcgca ggaggcgcca cggtggaaga
    16681 cgtgcgcctc ctgcgccgcg ccgggggaaa ccgcctggag gtcaaggcct cggggggcat
    16741 tcgcaccctg gagggggctt tggccctcct cgccgcaggg gcggatcgcc tggggtgttc
    16801 tgccggggtg gccctggtgg aggccctgga ggactccgcc cccggggccc catcgtgacg
    16861 ccccccgggc ccgacggatt ttttgcggcg cggaggcttt gccccgggga agaggggcgc
    16921 taaagtaaaa aaaaggagcg tgatcgccat gtctttgccg ggggccgtgg atggagagcg
    16981 cctcggtcgt cgcctggagg agctcggggc cgtgggacgc gtggtgcggg gaggggtaga
    17041 gggggtctgt cgtctcgccc tgtcggagga ggaccgccgg gggcgcgatc tggtgatttc
    17101 ctggatgcgg gaggatggcc tggaggtccg ggtggacgcc gtgggcaaca tcttcgccct
    17161 ggagccgggc cgggaggggg agatccccgt gatggcgggg tcccacatcg atagcgtgga
    17221 tggggcggga aagtacgacg gatgttacgg ggtcctcgcc gccctggagg ctctccgggc
    17281 gatccgacac gagggggggg cccggcgccg tccgctggcg gtgggggtct tcaccaacga
    17341 agagggggtg cgctacacgc cggacatgct ggggagcctg gtgcacgctg gcggcatgac
    17401 ccccgaggag gcccggaatc tccggggatt tgacggcacc gtggtggggg aggagctgga
    17461 ccgcatgggc tattcggggg attttccctg cgggagcctg atccccgccg cctatgtgga
    17521 gctccacgtg gagcagggtc ccgtgctctt tcgcgaggga acccccctgg gggtggtgga
    17581 ggtggtgacg ggcatttcct ggcaggaatt cgaaatctcc ggcacggcga accacgcggg
    17641 gaccaccccc atggaggggc ggagggacgc ggcggtggcc gccgcccggg tgatcctctt
    17701 cgtccgggat ctggcccggg agatgggggg cgccgcccgg gccactgtgg gcatgctccg
    17761 cctggaaccc gggggagtca acgtggtgcc cgaccgggcc ctcttcaccg tcgacctgcg
    17821 ccacagcgat gcccgcgccc tcgaagagat cgaagaaaag gtggcggcct tcggggagga
    17881 cgtggcccgt caggaaagag tggcggtgga acgccgctcg ctggcgcgct tcgaccccgt
    17941 ccccttcgac cccggggtga tggaagccat ccgtcgggcc gtcgccgacc tgggcatggc
    18001 gtcccgtccc atgatctccg gcgcggggca cgacgcccag atgatggccc ggatctgtcc
    18061 cgccgccatg atctttgttc ccagccggga tggcatcagc cacaaccccc gggagtacac
    18121 tcccccggag gacctggagc ggggagcccg ggtgctgcga cggaccctgg agatcctcgc
    18181 cgaaagagac taggaaggca tcccggaggc gaggtccgcc ctcgggacgc cttcgggtcc
    18241 ccgggggaag gagacgaggc cttcgcttcg gtcgatgaac cggaggagaa ggccccaagg
    18301 gagaggagga aaatcatgaa gatctacctg gcaggatgcg ttttggcggc ttttttggcg
    18361 gggacggcct ggggtgccac ggtgacctac cgcgaagagc tttccggggc ggcgaagacc
    18421 gtggtcccgg tggtccaggg aagcgaaggc gaggcgggcg cggcggtgaa cgcctcgttg
    18481 gcctccttgg tggaggatca ggtgcgatct ctgaaggacg gagatccccg agagctcatc
    18541 gtcgaggggc gcgcggagct tttggagggc cccctgtgga gcttttcctt cgagggcatg
    18601 gtggatttcg aaggggcggc ccacccctcc agcaccaaga agggggtcac ctttgacgcc
    18661 gcctcgggaa gggccctgtc gctggaagag ctcttccttc cgggcacgga ctgggcccgg
    18721 gcggtgaacg cccagcttgc cctcgccatc gaccgccagg tggccgaaga cgacctcatg
    18781 ctcttcgacg aagggcatcc gaacgtggcc gagtacgcgg agcagttttt tctgcgcccg
    18841 ggggccctgg tggtcttttg gaccgacacc cagtttgccc cccacgcctg tggccagccg
    18901 gagttcgtcc tcccccggaa cgcgtttgcc cccttcctcc gtccggagta cgcctcctgg
    18961 tgaccttccc ttgacgaccc ccggtcccgg ctctcgggag gatccccggg ggatttccgg
    19021 gagaccctct tcctggtcgg aggcccccgg agcgacgagc tgggcgacct ggccgatgct
    19081 ctggcggtca tggtggattc gatccgtcgg atgatcaaag accttcagtc ccagggggag
    19141 gccctttccg ggagcgccga tgccctggcg gccctctccc aggaatccgt ggccgccgcc
    19201 gaggagatgc gagccggggt ggaaagcgtg gcccagaggg ggaccgaaaa ccgggccagc
    19261 ctctcccgga gcggtgaagc ggtgcgggcc ggggcgaccc aggtggacgc ggtggcccag
    19321 gtggcggagg agggggcccg gagggcagag cagacggcgg agatgaccga aagggcctcg
    19381 gaggaggtgg cccaggccct ggcggcggtg ggggccgccc aggaaaggac tcgtctcgcc
    19441 acggagcgca tggaggccac cgcccaatcc gtggcggcca tcggtgattt cgtcaccacc
    19501 atccgcacca tcgccgacca gacgaacctg ctggccctga acgccgccat tgaagccgcc
    19561 cgggccggag aggcggggcg gggattttcc gtggtggcgg aggaggtgcg caaactggcc
    19621 gaacagtcca acgacgcggc gaaggaagtg gaaagccgca tggaagccct ctcgacgagc
    19681 acggcggggg ccctggatgc cattcgcctc ggcgatcagg tgatgggaga cgccaccgtc
    19741 cgggggaacc aggcccgggg ggtgttgggg gaagccctgg cgtcgtcccg ggagatcgtc
    19801 cgggccaccc gggaggcggc ccaggcggtg atccagcacg gcaaagagag ccagcacatc
    19861 gcccaggaga tcggccacgc cgaggagagc acgggggaga ccctcacctc tctggaaggc
    19921 attcgcgtcg ccatcgccga gaccactcga tcctcggagc aggtggccca ggaatcctcc
    19981 cgcatcgccg aagaggcgga ccacatgcgg gagctcttgg gtcgcttcca caccgaggcg
    20041 agagccctgg agtgatcccg acctcgccat aaatcgaaag aatcgaaaga atcgaaagag
    20101 acgccccggg gccctttagg gccccggggc gtctcttagg ggcgttggtg gcgggaaaaa
    20161 aaccgccaga gcacccggga ggcctcggga ctcttggggg gctcgtcgca gtagcggtac
    20221 ttccgggggt ttcccccggg ccaggcgtgc cccgcgccgt cgatgaggtg gagctccact
    20281 tccgctccca tccttcctcc ggtccatcgc aggatggtgt cttggccgcg ccgatcctta
    20341 tggggggtgg gatcgttgcc gttggccgcc gcccagaatc gggcggcgct ctccaccgcc
    20401 tcccccgggg gacggcccct ctgggggcgg gcgaaagact tcccgtccgg gggcaccacg
    20461 ccgtctttcg tgccgtgaat gatgcacacc gacagcgctt cccgagcttt ccggtctccg
    20521 ccccgcaggg cccccgccac gacccctagg gccgccaccc tccccgggcg ttccagggcg
    20581 tagcggtagg ccatcatggc gccgttggaa aagcccgcca ggtagatccg ccggggatcc
    20641 acggggaagg tgcgttccaa aagatcgagc aacgcgtcga gggcccccac gtcgtccacc
    20701 cgacgatcca gggcgtaacc gcagcagtac caagcattcc acgtgaggcg gtcggggcgg
    20761 gcccccgtgc cgtcggggaa ggccacgatg aagccctctt cgtccgccag ggcccccagc
    20821 cccgtctgct cccgccccag gacccccgtc cccccggcgc cgtgaaggaa gaccaccagg
    20881 gggaggagtc ccggccccgg ggcccccgag gggaggtgga ccgtggcggt gcgatcccgc
    20941 gcctcccatc gccaggagac ctggcgatgg gaggcgtcgc cttcggaggc cccccccacg
    21001 gccaatccta aaaggagggc gaggagaagg ccccttcccc gaaagaccgg ggatggggcc
    21061 ggggaatccg cgaaaacccg ggctccgacg gggcctttcc cctccggagg ccccccggcc
    21121 tctttcgttg tgcccctttc ccccctgtcg ggccttggcc ccgggaggac gatggtctct
    21181 tttcccatga tgcgccccct ctcggacctc catggtaccg cgtctccccc agaggtcaat
    21241 gctccgaacc ctccgtccgt tgtgtccgag atcttctcgc gctataatgc ccctgcgggg
    21301 cagaaggcca ccgcagcaga ggggaaacaa agagagtggg gaagagaaaa actagggagg
    21361 agagtggtga aaatgaacct caggcggaga tgggggatcc tactcggggc gatggtgtgg
    21421 ggggcggctc ttttcggggt cgccgaagcg ggggtaaagg tggtactgaa gaacaactcc
    21481 ttcgagaaca tcgtggtggc gatcaattac ttggccccca acggccgatg gatcacccgg
    21541 ggttggtggg acgtcccctc gggaaagagc gtggagccga atgttacctc cgagaacacc
    21601 cacttctact tctacgccga aggggacaac ggagggcgat ggaacgggga ggacgacgcc
    21661 gagggcatct cccggtgggt ggtggatgaa aagttcgacc actacgacga tcagaagggc
    21721 aatcctgcgg ggtccaacgg gcgccaggtc tcctttttcc acgccgaggc gaaaaatggc
    21781 gtcgtggagc agaagttttc cgactgaggt gcgcaggtat aaaaagattc taggtgggcg
    21841 ggggcttttc ccccgcccat cgctttagtt ctcgggccgc cggagctcgg tcagcccctc
    21901 ttccacgtaa tttcgcaccc agtgcgccac ggcgggggtc tgaaagagcc gggggtcttc
    21961 caggatctcc accagggcgt cccggtccac ctcgaatacc ccttcccccc gcaggaagcg
    22021 aaaggtccag gatcggtcgg gacttccggc cagcagggtc accaccgtgg cggccagatc
    22081 ccccagaggg gggcagtcca ccgcgtcggc ccgaaggcgc gccgccaggg tggtgccctt
    22141 tcccttctcg gaggcaagat ggagctcccc gtcgcagagc tctgccacct gcttcaggaa
    22201 ggggatgccc agccccaccc tccgggtggt gcgggacgtg acgaagggat ccgtcacccg
    22261 ggccaccgtc tcgggatcca tgccgccgcc gtcatcttcc acccggagct cgacccaccc
    22321 ctcccggtcg cgctcctgaa gggtcaccac gatgcgccgg gcccctgccg ccatgctgtt
    22381 ttccgcgatg tccagcacgt gttttgatag atcgtccagc accccattcc ctccttgccg
    22441 ggattgatct cgagagatcc cctggcctca ccccttcagg ggtgcggggt ctcctgccaa
    22501 aagcggaacg tccctcggcc cgcccccttg gcggcataca tggccatatc ggcacgcttc
    22561 atgagttctt tcaccgtggc gccgtgggcg gggtagaggg cgatgccgat gctggcgccg
    22621 atgtggatct ccttgtcctg gatgacaaag gggcgcttca gctcctccag gatgcgctcg
    22681 gccaccgttc cggcggcgtc ggggtgctcc aggtcgggga gcaccacggt gaactcgtcg
    22741 ccccccatgc gcgccacggt gtccatctcc cggacctccc cttttagacg ttccgccacg
    22801 gccttgagga ggaggtcccc cacgtcgtgc cccagggtgt cgttgacgtg cttgaattgg
    22861 tccaggtcca ggaagaggag tcccaccatg ctccggtttc gtcgggcgtg ggccagggcc
    22921 agggccagac ggtccgagaa gagcatccgg ctggggagcc ccgtgagggc gtcgtgggtg
    22981 gcctggtacc ggaaccgggc ctccaacatc atgcgttcgg tgatatcccg ggcgatgacc
    23041 tcacacccca acaccttccc ctggtgatcc acctcgggta ggacggagta gtccacgatg
    23101 aggggtcggc cgtcccgggt gcgccagtgg atgacctcgc tcgggatgcc ctcctctccc
    23161 catcgggctc ggtcgatgag ggagttaacc ttctttaggg attcggcgtc gaagaccttc
    23221 tccggcagga gggggtcttt ctgccactcc gccagggagt agcccagcat ccgttccgcc
    23281 gccgggttga gatagaccac ccggctagtc tggtcggggg agaaggccat ccggaagacc
    23341 gcctcgtggg cgttggcggt gagccggtca tagcggtcca gcacctcccg gagctcgctt
    23401 tcggcccggc ggcgctcggc gatctcctgg cggaggtcgt cgttggcccg ggcgagctcc
    23461 cccgtgcgct ctctcacctg ctcctccagc tgatctcggt agcggcacag ctcctcttcg
    23521 gagatctgga gggatgccac gtggctctgg aggatggcgt tgaggcggtg gatgtgctcg
    23581 ttgagggcga agctctccag gacccccgcg caggacagga gggtgaggga gaggaagtcc
    23641 atggccaggt cggggatctc gtgctttttc atccccagga cccccaggaa aaatcccatc
    23701 acgccggtgg gcgtggccag ggagtggatg aggatctgct cgaggccgtc cgtggccccc
    23761 acgacgactc cccggcggcg tcccagggcc caggcgatgg tgcggtcctc caccaggggg
    23821 ggcctttcgc gctcgaaaaa ttttctccac tcctcggggt ggcagcggac gagggagaag
    23881 ctggcgtccc cttcgtccac caggtagaac gctgcggcct tgaagggcat catggtccgg
    23941 atcttttcct ccgtctcccg gagcaccgcg tcccggtccc tcagggtgac gatggtggcg
    24001 ggaaaggcgc cgagccccag ggccatgtcc agggcctcca tggcggcgta gagctcctcc
    24061 gatagaaagg ccacccgctc ctccaggtgg cggagtttgg cgtggggata tctcacccgg
    24121 tcacacctcc tcgggggacc ggggggcggg ggaaagcccc tcccgggggt ccccgccgtc
    24181 ggcgccgaac atctccagga cctcggcgat ctggcgttcc gactgggtca cgaaggtttc
    24241 catcgactcc ggcgccaggt ccacgaggcg ccaggcttcc tcgtccagag gaggcaccac
    24301 ctgctccggg tggattccct ctcccagggc gatggccgcc aggtccgcca ggtggagcag
    24361 ggcgggttcc atcgcctggg gggcccgggc cggggcgtga tgccacgcca cggcgccctc
    24421 gatggcggag gggatctgcc accgtcggag caggagtccc cccagccggg cgtggtcata
    24481 ccccaggacc ccttcttcgg cctcgtggag cggcatgccc tcccggcgct gccgctcgaa
    24541 ggccagggtc atggccccgg ggaggcgtcg caggagcacg atgcggccga tgtcgtgcaa
    24601 gatccccgcc acgaagaccc tttcctgaaa tacctccggg tgggcgatgg aaaagaggcg
    24661 ggccagcacc cccgtggcca cggagtggcg ccagaagctt ttcatggaca cgaaggactc
    24721 cgggacgtcc gggaacatgc tcacggcgga aatccccagg gcgatggtgg agagctcgtt
    24781 tcccccgata agggccacgg cccgggggag ggactcgatg cgggagggga agctgtaaaa
    24841 ggcgctgttt accagcttca gcagcttcgc ggtgaggctg gtgtcctttt ctaccacgct
    24901 ggcgacgtgc caggcggagc tccggggaga gccgatcacc tccacgatgc gaaagtaggt
    24961 gtcaggaaaa gaagctagac ttacctcctc cgcgatgagc ttatcggcgg gggggatctt
    25021 gccgggggat cgccgcgagg aagggtctcc ggaagaggcg ggggatcggt cggaaagttc
    25081 ccccctcgcc agacgaaggg cctcccttgt cacagcgatg cggtataatt cttccaggaa
    25141 gggatgggcc gtgttgccgg cggcaaagcg ccgatccacc cgtcgctgcc cctcctcgaa
    25201 gcaggcggcg gggacccttt ccccgaggga ttcccgggga tcctgggggg aagagttttc
    25261 gatgtcggcc ccggcgatgc cccaggtctt gatggtctcg atgtgtttgg ccgagagcgt
    25321 ggcccctttg gagagcacga gacgcccccc gggggcacga agatctttcg ccagcaccat
    25381 gccgggccga agagccccgg cgctgaggat acccatggga ccgcctcctt tcgggggacc
    25441 tttccccgga aacattatat cgcaacggcg cccgttatgc ctctagagaa aggggagatc
    25501 gacaagtgaa ttcgcgtctg gaacttcgtt gcgtgctctg cggccggagc tacccccccg
    25561 acgccgcgac ccacacctgt gcctcctgcg gcctggacgg caccttggac gtgctctacg
    25621 acctggacga ggtgcgtcga tccctctccc gggaggggct ggcgcgggac tttcgcccct
    25681 gtttatggcg ctacgccccc ctactgccgg tggaggagga gcccttcatt ccccccctgg
    25741 cggtggggtg gtctcccctt tatcggagcg accttctggc aagacgatac ggcatcaagg
    25801 ccttttatgt caaggacgat ggaagaaatc ccacggggtc cctcaaggat cgggcctctg
    25861 ccgtgggggt ggcccgggcc cgagagctgg ggcagagcgc catcgcctgc gcctccacgg
    25921 gaaacgccgc aagctccctg gcgggctttt ccaccgtggc ggggcttccg gcgtacatct
    25981 tcgtcccgga aagcgccccg gagggcaagg tggcccagct cctcatctac ggcgccacgg
    26041 tggtgctggt gcggggagac tacgccgccg cctttgacct ggccaccgcc gccatggatc
    26101 gcttcgggtg gtacaaccga aactgtgcca tcaacccctt cctggtggag ggcaaaaaga
    26161 cctgcgccct ggagatggcg gagcagctcg cctgggaggt ccccgaccgg gtgttcatcg
    26221 ccctggggga tgggtgctgc ctcagcggtc tgtacaaggg ctttcgcgac ctgaaggagc
    26281 tggggttcat cgagaagatc ccccatctgg tgggggtcca ggccgagggg gccgccccca
    26341 tcgtccgggc ccttcgcgac ggcgcctccc gggtcttgcc ggggaccgcc gtcaccctgg
    26401 cggatagcat cgccgtggga tccccccgga actgggccaa ggccctgagg gccctccggg
    26461 agagtgccgg ggccggggag acggtgacgg acgacgagat cctgggagcc atgcgagagc
    26521 tggctcgcag cacgggggtc ttcgccgaac ccgccggagc ggcggccttc gcgggctttt
    26581 gcaaggccgc ccgggagggg cgcgtggcga aagacgaggt cgtggccgtg gtggtcacgg
    26641 gaaatggcct gaaggacgtg cccagcgccc ggagggccac gggggctccg ctggtggtgg
    26701 accccgatct gggcgatcta ctacgaaaga tggccgaagg gggggcgcga tgactcagga
    26761 aggcggggag aagggcccgg aggggcccag agggaaaggg gtgcctcggg atctccgggg
    26821 cccttcgtcg cccccgacgg ggcacaccaa cttaggaggg atcctatcat gatcgatctt
    26881 accatccacc ccgcgccgcg ccgggcggcg attcggcggg cccgggagag gggcatcgtg
    26941 ctccccacct tcgaggagat gaagcacccg gagaagatct cccccaagat caaagaacgc
    27001 ctccggcacg tggggctatg ggatctggac tccgccaacc tctttcgcat atcctggaaa
    27061 aatgcccccc agccctcggg gggactccac ggaggggtga actacttcga gattccttcg
    27121 gcgctctcgg gggtccgggc ccgcatcgtg gggttggtgg gcaagtggtt ccccacgggg
    27181 gcccataaag tgggagcgtc ctatgcctgc ctggtgccgc gcctggtgac ggggcagttc
    27241 gaccccacgg cccaaaaggc cgtctggccc tccacgggaa actactgccg aggaggggcc
    27301 tacaacgccg ccctgctggg gtgcgagtcc gtggccatcc tcccggcggg catgagcccc
    27361 gagcgtttcg agtggctttc caaggtggcg ggggaagtca tcgccacgcc ggggagcgag
    27421 agcaacgtca aagagatctt cgacaagacc tgggagctcc ggcgcacccg ggacgacgtg
    27481 gtgatcttca accagtttga cgagatggga aatcacctct ggcactacga ggtcaccggc
    27541 ggcgccctgg aagaggtctt ccaggccctc cggggggacc gggggcgcgt ggcgggaacc
    27601 tttttcacct ccggatcggc ggggaccctg gcggcgggga acgccatgaa ggaccgccac
    27661 cctcgctcca agttcgccgt gggggaggcc ctccagtgcc ccaccctgct gaaaaatggc
    27721 ttcggcgccc accgcatcga gggcatcggc gacaagcacg tcccctggat ccacgatgtc
    27781 cgggagaccg acctgatcgt ggccctggat gacgaggact gcatggccct ggtgcgcctc
    27841 ttcaacgatc ccgccgggcg ggcctatctg aaggggcggg gcgtggcgga agaggtggtg
    27901 gatcgcctcg acctcttggg tatttccggc gtggccaatc ttttgggagc catcaagatg
    27961 gccaagtact acgagctctc cgaggacgac ttcgtcttca ccgtcttcac ggactccatg
    28021 gagctctacg gctcccggct ggaggagatg gccgaggtcc gggggcccta cgacgccgtc
    28081 caggcggcgg tggactgcca tgcccacctc tgggggataa aagacgatgg gttgatcgag
    28141 ctcacctacc ccgagcgcca gcgggtgcac aacctcaagt actacacctg ggtggaacag
    28201 cagggaagga ccctggagga acttcaggcc cagtggtacg attacgatgc ctactggggg
    28261 aagctccgcc gtcaggcggg ggccatcgac cgcctcatcg aggacttcaa ccgggaaagc
    28321 ggagccctgg agcagctcgt ctagacgggg cgggaccgac tagaccaact caaggaggaa
    28381 tggccatgac gctggacttt gccgccatac gccagcgggc ggagcaccac cgaccgaaga
    28441 tcgtgcgctt tctgcgggac ctcatcgccc ttcccagccc cagcgggggg gaggaacggg
    28501 tggtggggcg catccgggag gagatggaag acctggggta cgaccgggtg gagtacgacc
    28561 ccctggggaa cgtgctgggc tacctgggat cgggcccccg gctggtggcc ctggacggcc
    28621 acgtggacac cgtgggggtg ggaaaccggg acctctgggc cttcgacccc tacgagggct
    28681 ttgaagacga ggagtgcgtg ggggggcggg gggcctcgga ccaaaaagga ggcgtggcct
    28741 cggcggtctg ggcgggaaag atcgtccgag atctggggct tttgcccccc gacgtcacct
    28801 tgctggtcac cggcacggtg caggaagagg actgcgacgg cctctgctgg cagtacctca
    28861 tccgggagca cggagtgcgg ccggaattcg tcctctccac cgagcccacc tcctgtcgca
    28921 tctaccgggg gcaccggggg cgcatggaga tcaagctctc cgtggcgggg gtctcctgtc
    28981 acggctctgc ccccgagcgg ggggacaacg ccatctacaa gatggccccc atcctggaag
    29041 agctccagac cctccacggc cgcctggcgg accacccctt cctcggcaag gggagcctga
    29101 cggtgtcgga gatctggtcg tcgtcgccgt cgcgctgcgc cgtggccgac ggctgcggca
    29161 tctccatcga ccggcgcctg actttgggag aaacggcgga gatcgccctg ggggaaattc
    29221 gcgatcttcc ctccgtccgg gccgcccggg gcgccgtgga gatgtatgat tatgccaccc
    29281 cctcctggcg aggggtgacc taccccacgg aggcctactt ccccacctgg ctcatcgaag
    29341 aggatcaccc cgtgacccgg ggggtggtgc gggcccaccg gggactcttc ggggagcccg
    29401 tggtggacaa gtggactttt tccaccaacg gcgtggccat catggggcgt ttcggcatcc
    29461 cctgcatcgg cttcggcccg ggacacgagg accaggccca cgcccccaac gagcggacct
    29521 ggaaagacga gctggtggcg gcggcggccc tctacgctgc cgtgcccgcc acgctggagg
    29581 atcggaccgc cgggacgtag ggggcgtggg ggcaaaaggg ggccttggag gcgcattttt
    29641 cgggccctcc tagccccgga gcgaaagttt ttccggatgt ggtgagagat cgttttttac
    29701 cccgagagga gaggatgggc cgtgcagagc atctttcgaa agaagcactt tatcaccctg
    29761 caggaatgga cccgggagga gatcgacacc ctgctggagg tctcgggcga cctgaagcgc
    29821 cgcttcgccc tgggggagcc cacgcccctg ctcccctatc agactgtctt tctcatgttt
    29881 ttcgagcagt ccacccggac ccgaaactcc atggaggcgg ggatcaccca gctagggggg
    29941 cacgcccact atctggacac cagcaccatg cagctctccc acggggagtc ccccaaggac
    30001 acggccatca tcctatcgcg tttcggccac gccatcgcct gccgaaactg cttctggaag
    30061 gtgggcaacg cctacctgcg ggagatggcc cgttgggcca cggtgcccat cctgagcctc
    30121 caggatgacc tctatcatcc catgcaggcc ctcgcggacc tcatgaccat ccaggaaaag
    30181 cgggggaaaa actccgaacg cctcaaggtc tccatcatct gggccttcgc cacgagccac
    30241 aaaaaaccca tctccgtgcc tctttcccag gtgctcctct tcccccgcta cggcatggac
    30301 gtcaccctgg cgtaccccga ggggtgggat cttcccgagt gggtcatcga cgaggcccgg
    30361 aaaaacgccg cccgtcacgg gggctccgtg cgcctcaccc acgaccagga agaggcctat
    30421 cgggacgcgg atgtggtctt ccccaagaac tgggggagct gggtgaaaaa cgagagcacc
    30481 acagtggtgg atggggccct ggaggcccag cgccactgga agtgcacgga gcgcctcatg
    30541 gagctggcct cccgggactg cctctacatg cacgccctcc cggcggaccg gggcaacgag
    30601 gtggaagacg ccgtcatcga cggcccccac tccgtcgtgt acgacgaggc ggaaaaccgc
    30661 ttgcacaccg ccaaggccgt catggccctg accatgggcg ggcgctaaaa ggatcggcct
    30721 aaggaatcgt ttctcaaaga ccattggaga ggagggatcg ccgtgtccct gacatggtat
    30781 gttccccggg aagtgcaaga ggtgcccgag ctattggctc gggagggagt gtatccccac
    30841 gggggaggga ccttgatcct tcggggcaag ggggcgaaga tccgaggtct ggtggacctt
    30901 tccctcctgg ggctcgatgg ggtgcgcttc gagcaaggca ccgtggctct cggggccatg
    30961 gtgacctttg cggggtgcgt ggcgcgcctg gggcgccacg atccggacca catcttggtg
    31021 aaggccctct cccgggcggc gtccactccc ctgcgaaacc gcatcacctt gggggggagc
    31081 gtggcggcgg cccccatctg gtccgatctg ttggggcccc tggtggccct ggatgcggaa
    31141 gtgatcctcc ggggccccga ggggagtacg gtcacccccg tggcgtcctt tgtgcgggaa
    31201 ggggcctcgt ggcgcggtgc cctggtgacg gggctccgct tccgggagga gggatggcgg
    31261 gccttctatc accgagaggt caccgtggcc ttcgattccc ccgcctttac cctctccatc
    31321 ctggcccggg tctcccccgg ccgggtggaa gagctccggg tggtgctcac gggatcccga
    31381 gaacgcttca tccgcctggt gacgatggaa gagctcattc aaggcaaaga cccctgcggc
    31441 gtcaaggtgg agggattggg ggagcgcttc gaggcggcct tccctgaccg gcgccatggc
    31501 agcggggcgt ggctccgcac cgtggcgggg gtcgccctgg agcgggggct tagggaggtg
    31561 ttgggaccat gctgaacgaa gaagatcgtc ggatcaccgg gaccttttcg gtcaacggtc
    31621 gttctcggag ctggagcttt tccccggagg ccacgttgct ccatgtcctg cgggatcacg
    31681 gccacaccga ggtgaaaaac ggctgtgaag agggggagtg cggcgcctgt ctggtgcttt
    31741 tggacggcaa gcccgtgcct tcgtgccagg tctttgccgc caccgcccga gaccgggaga
    31801 tcaccacggt gcgggccctg gggaccatcc acgcccccca tccgatccag gaggccttcg
    31861 tggaggtcgg ggcggtgcag tgcggctttt gcaccccggg gatcgtgctg gccacggcgg
    31921 ccctgctccg agagaacccg aatccctcgg acgaagacat catccacgcc ctggacggcc
    31981 agaagtgccg gtgcacgggg tacgtgaaga tcatcgaggc cgtgcgcctc gcggcgagga
    32041 ggggtcgtca tgagtgagtt tcaggtagtg gggcactcgg tgaccaaata cgacgccctg
    32101 gccctggcca cgggggaggc ccggttcacc gacgacttcc cccacgaggg cttcctcgag
    32161 ctggccctgc tctggtcttc ccacgcccac gcggagatcg tctccctgga tacctcggag
    32221 gcggagggcg tgccgggggt ggtggcgatc ttttccgccg ccaacgtccc ccgggtgctc
    32281 tacaccagcg cggggcaagg ggctcccgag ccctcgccct acgacatggt gctctttgac
    32341 accaccgtgc gccacgtggg ggaccgagtg gccctggtgg cggcggaaag cctcgccgct
    32401 gcccgggagg cggtgggcaa gatcagggtg gagtggaagc ccttgcctcc cctcttcgac
    32461 gccgaggccg cccgggacga aggcgccccc aagctccacg gcggggacca ccacgctccc
    32521 ctgccggtgc cctacgaccc ggggcacaac ctggccgcca gcgtgtcctt cgactttggc
    32581 gaccgagaag gggccttcga ccgggcgacc cgggtggaag agcacgaata ccgcgtgcag
    32641 tacgcttccc actgcgccct ggaaccccat gcggccctcg cctccttcga cgagcgggga
    32701 cgcctggtgc tggtgagctc cacccaggtg cccttccatg cccggcgcat caccgcccgc
    32761 gtgctccaga tccccacggg gatggtgcgg gtgatcaagc cgcgcatcgg cgggggcttc
    32821 ggcggcaagc aggaagttct catcgaaccc ctggtggccc tggcggcctg gcgcaccaag
    32881 cgcccctgcc gcctggtgct ctcccgggag gaggtcttcg tctcgggacg cacccggcac
    32941 cccgtgcgcc ttcgccttcg caccggggcg gacccccggg ggaacctcac cgccttggac
    33001 ctggatgccc tcatgggcgc cggggcctat ggcccccacg ccctcaccgt gctctccatg
    33061 gtggggggga agaccctgcc cctcttcaac aagatccccg ccatgcgctt ccagggaaat
    33121 tcggtgtata ccaacctccc cgtgggaggg gcctaccggg gatatggcgc cacccagggc
    33181 tttttcgccc tgaaccagca gatcgacatg catgcccgag aggctggaga ggacgtgatc
    33241 tcctggatca agcgccagac catccgggcg ggggagacat cgcccgtttt caaggccctg
    33301 ggggaaggca aggagggcgt ggatcagatc gtgcgctcct gcgccctgga ggcctgcctg
    33361 gaccgggggg ccgaggcgat aggatggaag gaaaagcgag gacgccgcct ttgccccacg
    33421 ccggacaccg tgcggggagt gggggtggcg gtggccatgc aggggtcggc catacccagc
    33481 atcgacctgg cctcggtgtc cctcaaaatg aacgaagacg gttcttttaa tctactggcg
    33541 ggggcggcgg accttggcac cggggcggat acggcgctgg ggcagatcgt cgccgaagtg
    33601 ctcaccgtgc ccacagaaaa aattgtggtg ctctcgggcg acaccgacgt gacccccttc
    33661 gatgtggggg cctacgcctc gtccaccacc tacctttcgg gaaaggcggc ggaaaaggcc
    33721 gcccgggatg tggcatccca aatcctcgcc gtgggggccc ggatgctcga agtcccccgg
    33781 gaagagctga ccctggcggc gggacgggtg tgccacgccc cctcggggcg gtcggtctcc
    33841 ctggaggagg tggcctgcga tgccctctac gcccgggatc agcaccagat ccaagcctcg
    33901 gcctcccacg tggcccccga atcccctccc cccttcctgg cccagttcgc cgaggtggaa
    33961 gtggaccggc gcacgggaca tctccgggtg ataaaattcg tatccgccgt ggactgcggc
    34021 cagcccatcc accccgccct cgccgagggg caggtggagg gggccgccct gaatggcatc
    34081 ggctacgccc tctgggagga gtaccgtttc gacggccagg ggcgcctcac caacccgaac
    34141 ttctgggact acaagatccc cggcgtgcgg gacctccccg agatggtcac catcctggtc
    34201 ccctcctcgg agccctcggg ccccttcggg gccaagtcgg tctcggagat cggcataaat
    34261 ggccctggcc ccgccatcgc caacgccttc ttcgacgcca cgggggtgag gctcttcgac
    34321 caacccttca cgcccgagaa agtccttcgg gccctccggg aagcgggtgg gtcatgatcc
    34381 tgcgaggggg gctcctggcc cttccaggag aggagcacgg agtggtggga gacgttcgca
    34441 tctcccaggg gcgcatcgtg gaaatcggcg aagacctggc ggacgacggg gccgtgatgg
    34501 acgcccgggg gctggtggtg ctccccgggg gcatcgactc ccacgtccac ttcaacgacc
    34561 ccggctacac ctcccgggag gacttctact gcggcacctg cgccgccgcc gccggaggga
    34621 tcaccacggt ggtggacatg ccctgcacct ccgtaccccc ggggaccagc cgggtgcacc
    34681 tgatggaaaa gttggcggtg gtgaagcgca aggcggtggt ggatttcggc ttcttcgggg
    34741 gcgtgtcggg gcagagctac caggacgccg tggggccggg gatggcggag atggccccct
    34801 ccgtgtgggg tttcaagacc tatctcacct ccggcatgaa gaccttcgag cggctaaacc
    34861 actggcaatt ccgctcttgt ctggaacggg cggcggaggt gcgccgcccc gtactggtcc
    34921 atgcggagga cttcgactac gtccaggccg ccaccgccgc atgcacccgc cggggggact
    34981 ccccccggct cttctaccag tctcgccccg agacggcgga aaacattgcc gtggcggcgg
    35041 cggtggagat ggccgaggag acgggagcgt cgctccacgt ggtccacgtg ggcagcgccc
    35101 gggcggcggc tttggtgagg gatcgggccg cctccgggcg gatcacctgc gagaccgccc
    35161 cccactacct gaacttcgac gtggaggact tcgtcgccct gggggccccc ctcaaggtca
    35221 ctcccccggt gaaggctccg gagaaccggg cctccctctg gcgcttcctc gccgagggga
    35281 gcatcgcctt cgtggcgtcg gatcacgccc cctgtcagga taaggataaa aagaccgggt
    35341 ccatctggac ggattactcc ggcatccccg gcaccggcac cctgatgccc tacctctttt
    35401 ccgagggata tctggcgggg aggctctccc tggggcgcct cgccgaggtg acctcggggg
    35461 ccgccgcccg gcgctacggc gtggcggagc gcaaaggggc cctggcgccg gggcgggacg
    35521 cggatttggc cctcttcgat cccgggcgtc ccggggtcgt atccggggcc cttttcccct
    35581 ccaaggggaa aatcaccccc ttcgagggga tgaccctccg ggggactttg gtggcgacat
    35641 ttctacgggg agagatggtg tttcaccggg accggggcgt ggtggccccg ccgggcacgg
    35701 gacggttcgt cacccccgcc ggggcgtagg cgttcccccc ttcccgaccc ggggcaaggg
    35761 ccaaaaaaac cgagtaagat aacccaagag atctgaaaag gagtgatgca tcgtgcactt
    35821 tcccagctct cccgacttgg cgctccgccg cactgtggag gaagacctgg attttgtcgt
    35881 ggcggccgaa aaccaccccg aaaaccgccc ctatgtcttc cagtggtccc gggagcgcca
    35941 ccgcgccgcc ctggccaatg gagacctgcg ccatctggtc ctggacgccc tgccggagcg
    36001 gcgccctgtg ggttacgcca tcctggcggg cattcaaaac gcccaccgga gcgtggaact
    36061 catgcggatc gtggtgacgg aaaaaagaag ggggtacggt cgggaggggg tgcgcctcat
    36121 ccaggagctg gccttcgact tcctgggggc ccatcgactt tggctcgaca tgcgcaccta
    36181 caacgaccag gcccgggagc tctaccgaag tacggggttt gtggaagagg gaatcctgcg
    36241 ggattgcgtc ctggtggacg gagtgtttta ttccaacgtc atcatgtcca tgttggaggg
    36301 agagtaccgg aggttatcca agaagtagcc cgaaaggcca tcgcccctcc ctcaaagcag
    36361 gggaggggcg atggccacca tcctttaggg ggtgatccca tggcccaacg ggccgtcacg
    36421 gtaaacggca tcactttttc taaccccgtc ctccccgcag cggggcccaa cgtgcgatcc
    36481 gccgccctgc tccaggccgc cgtggatgga ggagcggggg gcatcgtcac caagaccgtc
    36541 tcggtgcgtc ccgccgtgga cccccgcccc accctggcca agggctcggg agggggaatg
    36601 caaaactgcg agacctggtc cgagaccccc gtggaggaga tgcttgaggc ctatcgggct
    36661 gtcaaggccc ggggggtgcc cctggtctgc tccctggggt accaccccga cgagctccga
    36721 accctgggcc ccctgctgga acgggaggtg gcccccgacg ccctggagtt ttccgtccac
    36781 tacgtgggca aatccctgga tcccctgctg gaggtggccc gggccctgcg ggagagcgtg
    36841 tcgttgcccc tgtggatgaa gctctccccc tccatcggcg acgtggaagg attggcgcgg
    36901 gccgcgtccc ccttcgtgga cgccttcgtg gccatcaact ccgtgggccc cgccctggat
    36961 tttgaccctg ccgactgccg cccccggctg ggctcaccct gggggcaggg gtggctttcg
    37021 ggccccccca tcctccccct ggccctggcc ttcgtctttc gcgtggccca ggcccaaccc
    37081 aagcccgtca tcggcgtggg gggcatcgcc tcgggggagg atgcggcgaa gttcgtcgcg
    37141 gcgggggcct ccctggtgca ggtctgcacc gccgccctcc ggggagggcc gggaacctac
    37201 gggcgcatcg acggggagct ggatgcctgg ctcgatgccc aggggtacgc caacctggcg
    37261 gagctccggg gacgctacgc gacggccctg aaggggggaa ggccatgact cccaccggga
    37321 atgtcctggg atcgacccct cggcgtgcgg acgcctggga aaaggtctcc ggccgggcgg
    37381 aatacgtaga cgacctctcg atccccggcc tctggttcgg agggaccctg cggagcgccg
    37441 tcccccgggg gcggctccgg ggcatctccc ggagggagag cttcgactgg tcccgggtgg
    37501 tggtggtgac ggcctcggat ctgcccggcc ccaacgaggt ggccatggtg cgctccgacc
    37561 accccatcct cgccgccgat gccgtgaact tcgtgggcca acccctggcc ctggtggcgg
    37621 ccccggatcg ccagaccctg gcggcggccc tgggagccct tgtccccgac gtggaagagg
    37681 cccccgccgt cctcaccctg gaagaggccc aggaggggcg cacggtgatc tggggcgcgg
    37741 acaacgtgtt ggcggcctac gtcctcggtg acgaagaaga cgccgtggcc gaagcctttt
    37801 ctcgggccga tcgcatcgtg gaaggaacct acaccacggg ataccaggaa cacctctacc
    37861 tggagcccca gggcatggtg gccctccccc gggaggacgg gggcgtggag gtgacgggat
    37921 ccctccagtg cccctactac gtccaccggg ccctgcagcg ggccctgggg ttggaggatc
    37981 atcggatcgc ggtgcgtcag gccgtcacgg ggggaggctt cggcggcaag gaagactacc
    38041 catcggtgct ggcgatccac gccgccctcc tggcccgggc ctcggggcgc cccgtgaaga
    38101 tggtgttggg gagaaacgaa gacctgcgct tttccaccaa acgtcacccc tccgtggtga
    38161 gacaccgcac cggggtgcga tccgatggta ccctctgcgc cgccgaagtg gacgtgctct
    38221 tcgacggagg ggcgtacacc accatgagcc cggtggtgct ctcccggggg gccctccacg
    38281 ccctgggggc ctaccgggtc ccccgggcgc gcgtcaccgc tcgggccttg gccaccaaca
    38341 ccccacccaa cggggccttc cggggcttcg gcgcccccca gaccatcttc gccgtggagc
    38401 gccacatgga cctcatcgcc cgggagctgg ggatctctcc cctggaggtg cggcggcgca
    38461 acctcttgcg ccggggagat cgcctcccct gcggccagat cctcacggaa gagcccgggg
    38521 cctcgctggt gctggagcgg gcccaggccc tggcgaagta cgaaacccgc cacggcaccc
    38581 tccggaaaga gggccggggc ctagggctct cccttttcat ccacggcgga gggttcacgg
    38641 gatcggggga agaaaagatc gacggccggg tggccgtgga gatcggagcc ggagggcggg
    38701 tggaaatact ggccagcagt acggagatgg ggcaaggggc cgccacggtg ctcccccaga
    38761 tcggggccca ggccctgggg ttgcccctgg accgtatatt tcatcccgcc cccgccacgg
    38821 accgggtacc ggacagcggc cccacagtgg cctcccgtac cgtcatggtg gtggggcgca
    38881 tcctcgtggg ggcctgcgaa gatctggcgg cgaaggtggc tcgaaggttg gaggaagccg
    38941 caggactccc cgagggcagc gtgacgtggc gggaagggac ctttcgggga ccgggatttc
    39001 cggaaaagtc cttcgagggc gtgctggatc agtggaaaga tcggcttcag gggctgcggg
    39061 gcgagggacg atacgccccc ttcccggacc tgcactggga cgacgacgcc taccgaggag
    39121 acgcctacaa ggactactcc tggggggcca acgtggtgga ggtggcggtg gatccggacc
    39181 gccacaccct gaccctctgc gccgtcacgg ccgtggtgga gatcggccgg gcggtgcatc
    39241 ccttgctcgc cgaaggccag gtggcggggg gcacccttca ggccctgggc tacggcggca
    39301 tggaagacct ccgcatcgat gaccgggggc gctacctcca ggaccgcctg agcacctaca
    39361 gcatccccac cgccctggat gcccccgacc tggtggtgga gatcgccgag atcccctctc
    39421 cccgggggcc ctttggggcc aagggactgg gagagctccc cataaatgga ggggcccccg
    39481 ccctggcctc ggcggtggaa gacgccacgg gggtctttat ctccaccatt cccatcactg
    39541 gagagcgcct cttcgcccac cggcgcgacg ggctttccga tgaaccctcc tgaggaggtg
    39601 tggccatgat cctttccttt gcggtaaatg gaaaaacggt gaccgtggag ggccctcccc
    39661 tgaggcgtct gctggacgtg ctgcgggagg acctgggcct cacgggcacc aaggaggggt
    39721 gcggcgaagg ggagtgcggc gcctgcgcgg tgcttttgga cggccacctg gtgaactcct
    39781 gcctggtgcc cctcttccaa gtggcggggc gctccgtgga gaccatagag ggaatggccc
    39841 cccagggatc tcccgatgcc ctccagcggg ccttcatgga cgaaggggcg gtacagtgcg
    39901 gcttttgcac ccccgggatg gtcatggcct cccgggccct gctgagggaa aatccctccc
    39961 ctcgccggaa agaaatccgc acggccctgg cgggaaacct gtgccgatgc acgggctacg
    40021 aacccatatt ccgggcggtg gaaggcgccg ccgccgcagg gtacgaagtg gccccccccc
    40081 ctccggcccc cgagtttcgg gtgacctgta cggaagaagg gcgcatcgcc tttttccccg
    40141 agagcctccc cgaagccctg gaaatcctcc ggcagcaccc cgacgcggtc cccgtggcgg
    40201 gagccaccga cctcttgccc gacctgaccc gggggggtct cccggggccc catagggtga
    40261 tggacctcac ccgcctggaa gagctccggg gcatccgaga agaggacgga gtgctggtgc
    40321 tgggggcggg agagaccttc gcctccctgg ggcgttcccc cctggtgcgt cgcctcttcc
    40381 cctccctggg ggagtgcgcc gcctcggtgg gaggagtggc ggtacaaaac cgagccaccc
    40441 tggggggcaa cgtcatgaac gcctcggcgg cggccgattc tccccccgtg ctcctggccc
    40501 tcgatgcccg gggagtcctc gtctcggggg aggggacgcg agaggtcccg gccaaggagc
    40561 tgtggaccgc ctaccgccgc acggcccggc gccccgacga actcttgacc gccgtgaaga
    40621 tcccccgccc cgccccggga acccgggagg ccttcttcaa gctcggccct cgccgggccc
    40681 tcgtcatatc ccgcttgacc ctggtctgtg ccgcccggct ggaagagggg cgatggcgcg
    40741 gagtgcgcat cgccgtaggc agcgtggccc ccgtgcccca tcgattggag gagctcgaaa
    40801 ccttcctgga agggcgagtc cccgaagaag gagcggtgcg ggaggctcgg cgcctggcgg
    40861 aagacctcct ccgcccccgc acctcccggg agtaccgccg ggacgtgggg ggcaacctgg
    40921 tggggcgatt cctctcgtcc tggatctctc cccgctgaag ccccggccag aaggcttggg
    40981 agggcgaaag ggtcttgggg ggcggcccct tttcctgtat catggaggcg cccctttccc
    41041 ggaccccgga gggatgatct gcaggaaggg gtccacggtg agggccgaga tccccctttg
    41101 aaggaggtgg gaagccatgg ctggtcgcac caaaaacagc aagtattatg ccctcaaggg
    41161 cgtggtgaac gtgcgccggg aagaggccga cccattgcag gccttctttt acgccctgtt
    41221 cgacccgggg aacctgcatc gcttttccct ccgcctgatg tcgtcccctt ccatgaacat
    41281 caagcgcccc tgggacgagt atggcatcat gttggaagaa gccgaagaag accgggaaaa
    41341 cgccgagacg gaaggcacca tccggacggt ggtccatcag gtggtggaag ggatcaagtt
    41401 cgccgagatc tcgggggata accccatcgc cctgcagaag ctggagaccc acctcaaccg
    41461 ctccgtgggt tcggccctct cggggacggt gaacgccttc gtcaaggtgg aaagctgctc
    41521 caccgatgcc tttcacagct tcgtggcaaa atttaaccca ccggcggagg gctcctcccc
    41581 gccctcctcc cctgctggat cgccggaaga gggggcgggg gaaggggcgg gtggatccct
    41641 tttggaccgg gagatcgtcc tcgtgtgcca acccctggtg gatcccctcc gaggcaaggc
    41701 cgccaccgcc gtcctgccgg gggatgtgat cctggtcaag atccccgagg actcgatttt
    41761 tttcagcatg ctgactaaaa tcaagaaggg cttttttgac ggcgtggtgg aagccgtggt
    41821 gacccgggtg gatcgccatt cggcggagac cgtggtcatg gagaccgccc tggcggaaaa
    41881 cgtcaaggga aaggtgcccg cgccgaacgc gttacgggtg aagctgtctc cttccaccct
    41941 ccaggaagga ggaaagcccc ccagtgctcc cctggtgtgg gccctggtgg gaggaggggc
    42001 gctgatgctg ggggtgctcc tctggtatct catgacgtga tataatgcga tgcggcgcgg
    42061 cgatgacgcc caaaaacttt ttccgatgac ggcgcgaagg cgatcggccc cctcgtattg
    42121 atccagggct ccgaccgatc cggaggcccg agaaggccct aagccgccca acccttttga
    42181 ggaggatgaa catacatggc atcggacctg gaaattgccc agaaagtatc cctgaagccc
    42241 atcatggaga tcggcgccga cctcggcatc cccgccgaag cgatcattcc ctacggacac
    42301 tacaaggcca aggtggacct gcgcttcttg aagaccctga aagatcgccc cgaaggacgg
    42361 ctgatcctgg tcaccgccac cacccccaca gcggcggggg agggcaagac caccaacacc
    42421 attggcctca cccaggccct ggtgaagatg gggaagaagg ccatgatatg cctgagagag
    42481 ccctcgctgg ggccctgctt cggcgtcaag ggaggcgcgg cggggggggg atggtcccag
    42541 gtgctcccca tggaagacat aaacctccac ttcaccgggg acatccacgc cgtgggcacg
    42601 gcccacaacc tcctggcggc catgctggac aaccacctgc agcagggcaa cgccctgggc
    42661 atcgaccctc gcaccatcac cctccggcgg gccatggaca tgaacgagcg cgccctgcgc
    42721 cacaccgtca taggcctcgg gggcaaggcc cacggcgtgc cccgggaaac gggatttgac
    42781 atcaccgtgg cctcggaggt catggccatc ctctgcctgg cggaagacct gatggacctc
    42841 aaggagcgcc tggggcgcat cgtggtgggt ctcaacttca agggagagta cgtcaccgca
    42901 gcggacctca aggcccatgg cgccatggcc tccctgctca aggaggccat caatcccaac
    42961 cttgtccaga ccatagagca cgtccccgcc tttgtccacg ggggcccctt cgccaacatc
    43021 gcccacggta ccaactccct ggtggccacc cgggcggcgc tgaaactggc ggattacgcc
    43081 gtggtggaag cgggcttcgc ctccgacctg ggggcggaga agttcatgga catcgcctgc
    43141 ctctacgggc gcttctctcc gtccgccgtg gtcctcgtca ccaccgtgcg ggccctcaaa
    43201 ctccacggcg gcgcccccaa ggaccagctc ggcacccccg atcccgacgc cctgcgccga
    43261 ggattgccca acctggaggc ccacctggac aacctcaaca acttcggcgt ccccgtggtg
    43321 gtggccctga accgcttctc ctcggacagc gacgaagagc ttgccctggt gcgggaagcc
    43381 gccgcctccc gggacgcccg catcgccctg tcggaagtct gggaaaaggg aggagagggg
    43441 ggcctcgacc ttgcccggga ggtcctggcc gccatggacc agggctcggc ctatcgccgc
    43501 ctctacggcg agcaccttcc cctgaaggac aaaatcggca ccatcgtcac ccgggtgtac
    43561 ggcgccgaag gggtggactt catccccagc gcggcggcga agctggaaga gttggagcag
    43621 cggggctatg gcaccctccc cgtctgcatg gccaagaccc agatgtccct cacggacgac
    43681 gcctccaaga aggggcgccc ccgggggttt cgcgtcaccg tgcgcgaggt gcgcctctcg
    43741 gcgggggcgg gtttcgtggt ggccatctgc ggagacatca tgaccatgcc cggcctcccc
    43801 aagagccctg cggcggagcg catcgacatc gacagcgaag gaaacatcac gggactcttt
    43861 tagcccgaga gaagtaagct ctaaaaaaga tgggggcccc gaggtctttt cctcagaagc
    43921 ccccatcttt ttcccctcgc gggatcttcc cggagcgccc ctctttaggg cctagcgctt
    43981 gccgtagcgc tcctcgccgt aggagcggta ggtctttttg ggctctcccg aaagctcgga
    44041 agccgaccgt tgaaaggatt ccaagagctt tttgcgacgg ctctcctcgc cttccacctc
    44101 gcccatggcg ttatccgcgt cccggtcgag gcgcttttgg gcgatcagca cctccaggtc
    44161 cctcacgtcc acctccaggg cctcggccat ctccttcacg tcgagccggg ccttggggtt
    44221 atcccggatg aagtctcgag ccttggcgta gaccgtgtct aggcgtttca tgcaccggac
    44281 gcaaacccgg ggtcccgtcg acgcaaaggg tttattgcaa aggtcgcacg ttatcatttc
    44341 catgaccatg acacctcccg atcgtttata cccagtttcc cgccccgctc ggcagcgggg
    44401 cccccttcgg gagccctaaa gggcgcggta gaaggctaaa aggcagaccg tacgggtaaa
    44461 gtgtagcgac gggaagtggg aacgtcaagg atggggtgag ccaaaccaaa gacgggatcc
    44521 cggatcgaaa gcatccggtc cccggggcga tgggcggccc cgcagagttt cggtggacct
    44581 aaggagcgcc cccttctccg tcggctcacg gtctcatggg cgtgggaccg cgccccctgg
    44641 tcgggtcggg gataacggag gaacgccccc atcgacgaat cgacggaggg ggaatagatc
    44701 ccccggacag gggggccaaa gccccccgag acgccccgga gatcgcctcg agggaccttc
    44761 gacaaaaact ccctttcccc cttgacggat aatctctggg gtggtatcat tccccttgcg
    44821 ctgtc
//
LOCUS       DAOOER010000011        42870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_958_length_42870_cov_9.305851, whole genome shotgun sequence.
ACCESSION   DAOOER010000011 DAOOER010000000
VERSION     DAOOER010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 42870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42870
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_958_length_42870_cov_9.305851"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="PL162_02780"
     CDS             complement(<1..995)
                     /locus_tag="PL162_02780"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQF90990.1"
                     /translation="MMALFRNMTIRLRLLSLTALLCALTALASWVGYTRLTAAEENLN
                     TMYDKSLMTIQWVNDARSHVQAIRGNMLEAALAKDARQSGALLEDIQRRRLLNNENLE
                     NLAKAPLDDFEKKTLAEAQTHLVEYRHATDYVIKLLGENRHEEALALYGAKAKKPLEA
                     FNDNLKSIAAHAAKAAAEANAENEKHIAAALRFLLAVAAGAVLLGLALGLLSERAITR
                     PLGAVMEVLRKIAALDLRFDTSKGWLLNYQGNEIAAMVESTSALETGLKEFLVKVVSS
                     SGKLGETSEEFSALAEKSNAGVEESRAGADDVSSQMESLAAATQEITASVEEVASG"
     gene            complement(1149..2252)
                     /locus_tag="PL162_02785"
     CDS             complement(1149..2252)
                     /locus_tag="PL162_02785"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HQF90991.1"
                     /translation="MLQKERVEALARKLKQDGLRALIMGPSSDVEYLAGLELFDDERF
                     KAVGVTAEGQIFAIVPQIYEAEFARALGEDTPRYSWVDQNWFYGAFAAAVKDFGLEGA
                     PLAMNSGVRAVDAIEIAERHRVRLVNGWHCLDDLRICKAPDELALMRKAGRLADEVFA
                     DLVRFIKPGMKERDVRREISRLFDEKGADEEAFSSIVATGPNAAMPHYNSDDSTIERG
                     HFVVLDFGCRCGGYCSDTSRTVFVGEPTAEDRRIYEVVQKSQEAGEAAVRPGVLSQDV
                     DRAARQIIHDAGYGAHFDNRLGHGIGIAVHEAPNIMAGNTTPLRPGMTFSIEPGIYIP
                     GKLGIRIENIVAVTETGCESMNLSPRELVTIGD"
     gene            2606..2983
                     /locus_tag="PL162_02790"
     CDS             2606..2983
                     /locus_tag="PL162_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HQF90992.1"
                     /translation="MSSWHEELAQGGACGGVVSLPWRSSADVTLELRRGDRVRRGVGA
                     EGSPGWRLCTTEDGLEGWIHEDFLDPVGEEEAILREDYSSRELSLDEGERVWVHRAVG
                     GWLWVQVSQGAWGWIPEDQVRPD"
     gene            complement(3045..3974)
                     /gene="glsA"
                     /locus_tag="PL162_02795"
     CDS             complement(3045..3974)
                     /gene="glsA"
                     /locus_tag="PL162_02795"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HQF90993.1"
                     /translation="MTLPTETLLQQALDACRPAPGEGEVATYIPELAKGNPRHLGVAL
                     ATPDGAVASAGDGAVPFTLQSVSKVISLAGALELLGEERVFDAVGMDPTADPFNSIMR
                     LEMVKPHRPQNPLINAGALVVLSLLPHRDPEERFAFVRDWARRLSGNDALDYHEATYL
                     SEKSTSDRNRALAYFMRSVGTFRGDVENVLDSYFRQCSLRGTARDMAVMGMTLASGGI
                     NPLSGHRVLSERHALMTRALMATCGLYDGSGEFAVRVGIPAKSGVGGGILASVPKKVG
                     LGTFGPALDPKGNSLGGVRLLERLTQLLDLRIL"
     gene            4224..5315
                     /gene="buk"
                     /locus_tag="PL162_02800"
     CDS             4224..5315
                     /gene="buk"
                     /locus_tag="PL162_02800"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002834.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HQF90994.1"
                     /translation="MAFSVLTLCPGAQGTRMAEFCDDVALWEEEVPHAPPSSAPPGGL
                     REEAEVRCEALERFLEDHPLRGGGLHAVVGRGGVVGPVPGGFYEVDEALGRHLLKDHP
                     RGHVSNLGGVLARALALPRGARALMGDPLSTEEREPEARLSGWPELPWQALGHVWSLK
                     AAVRAAARDLGRPWEELAAVVVHLDRGISSCAHREGRMVDLINPNDGGAFSLDQAGGV
                     PSGDLARLCFSGAYTLRDVRTALVGAGGLGGYLGTRDLEEVHARRKAGDEGAERAFSA
                     MAYQVGQEIGALAVALSGRVDAVILAGPLARDPLLVQRVRGWVQWIAPVLVYPGGDEL
                     HFLAEGAVRVLSGEERAKCYGDYAGMEEE"
     gene            5320..6225
                     /locus_tag="PL162_02805"
     CDS             5320..6225
                     /locus_tag="PL162_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HQF90995.1"
                     /translation="MSWTSLQFLLDRCRDGRKMRVVAVCAQDEDVLFALDRASREGIV
                     DGVLVGSKAKIEGAASALGISLAPFEIVDAPTEYEASEQAVRLVSSGAADTLMKGMVK
                     TATLLKAVLNKEWGLRSGDLLSHLFMVEIPKLNRVVGHTDGGINLYPDLKTKVGILNN
                     AVDCYHKLGVACPKVAVLGAVEVVNPDMPCTVDAAALVQMNRRGQIKGCLVDGPLALD
                     NAVSLEAAKHKGIVSDVAGCADIFMVPNIEAGNLLGKVMLYMTGGVGAGVILGAQKPV
                     ILTSRFDSAETKLYSIALGAVLSQK"
     gene            6332..7252
                     /locus_tag="PL162_02810"
     CDS             6332..7252
                     /locus_tag="PL162_02810"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HQF90996.1"
                     /translation="MEQLRSLTALMEYAKQVGPRKISVAMAEDAEVLEAVEEARKAGV
                     ANAVLVGNGDKIAEIAGKLGIDLGHYEVVDERGGEPAVALAAVELVSSGKAEILMKGM
                     IKTANFLRGVLHKEKGLRTGSLISHVYVHHVEGYDRIFFICDPAFNMYPDLGEKVKIL
                     ENTVRLAHAFGVEKPKVAVLAAVEVVNPDMPCTLDAAAIAQMNRRGQIKGCIVDGPFA
                     LDNAISEESAAHKGIVSEVAGKADILLVPDIDAGNMMAKAIVYMTNNKTAGLVLGAKA
                     PVVLTSRSDSAETKLLSIASAVALAAYKGN"
     gene            7503..8570
                     /gene="buk"
                     /locus_tag="PL162_02815"
     CDS             7503..8570
                     /gene="buk"
                     /locus_tag="PL162_02815"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HQF90997.1"
                     /translation="MDYAIFAINPGSTSTKVAFFRGAEEIWSDVQRFDVETLRAFATV
                     ADQEDFRLEEIRKAMERHHVTEKDLDAVVGRGGLIKPIPGGTYEVSDAMVETLRSQKY
                     GAHASNLGAPLAMRVAQGKPAFIVDPVVVDELIPEARLSGMPQIERRSIFHALNQKAV
                     GRRAAADLGKPYEKANFVIAHLGGGISVGAHCQGRVIDVNNALDGDGPFSPERAGGLP
                     AGDLARLCFSGAYDLTEVIKKMVGGGGMVAHLGTNDLREAQRAAEGGDPKATLVLDAM
                     AYGVAKEIGSRAVALAGKVDAVILTGGLAYSEGFVRRIGDRVSWIAPVKVYPGEDELQ
                     ALAEGALRVLSGQEAPRVYEG"
     gene            complement(8656..11697)
                     /locus_tag="PL162_02820"
     CDS             complement(8656..11697)
                     /locus_tag="PL162_02820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQF90998.1"
                     /translation="MPGGEDLSPTPPLPEDVHADASAPEDTLRDSAIVGAFFEELFEE
                     ASEGIVFVDAHRRVIRSNRQFREMFQYSDEEIRGKIIDHLVTTSSEELAEAEDLSRRV
                     GAGEYVSLSSTFRSRKDGSRLPVALTVFPIKTSRGAFHYAAYQDISSLCLAEDSLRRR
                     LVLEKVLSRISARFLKDLCLDHAIHQALGDLGAFFSADRGYVFQVDPEGATHSNTHEW
                     CAPGISPAKDQLQRIAMDATPWWIDTLRREGAIIVPDVRSMPPEAAHEREILRAQGIQ
                     SLFVLSWGSGDSVTGYVGFDNNRHPGAWSQDDVEELKFFRDLLEHALKKRATERALRQ
                     SEKRYEAVVQDQDDLVCRFSPDGLLRFANRAFFSFFGLDRHALHAFSVFQCALPSAQE
                     PLREAMARIVLKSPRLLRTERMITRGGETRWLQWSYGGIFDDRGSLLEIQGVGRDITE
                     AMKTEQALRESRDLLEQRVEQRTAELETTNRELRQENARRKKTEDALKAQIRYTEAIL
                     RTVPDAVLVFDQGGTVTDVVTHMAPDQLPRFPSGHKLEDFLPPETVPRVREAIGRTLS
                     HLSLQTVEYTDEGGRSFEARLAPLCRGDHLCDRVVCLAIDITHRKEIEESIRAARDQA
                     EGANRAKSVFLANMSHEIRTPLNAVLGMARLLKDSLLSEDQQDLLQHILSSGEKLLSL
                     LNNLLTLARLETEGISPTPGPLDLRALVEKAGSETGQLFRRKGLTFRVLWDPRLPEKV
                     LGDEGLLRTCLDVLLHNAASFTSEGGVSLAISPEDPGGNPSLYRFSVTDSGIGIAAQD
                     LKKIFEPFTQLDDGLSKRHQGAGTGLAITKRIVQALGGSVSVESVPGKGSTFSFSLNL
                     PPASGPGPSGGFPLPPGLRVLLVEDNPVDRKLIQLMLRKDKAELRVAESGDQATEMLE
                     REPFDLVILDMPEGELGCLKDALSLRDQRYPSLSLVALGASARPEDSGRWFDAGGDGF
                     IPKPFTAETLHQALRQALDRRAPRRSPEKKAKKTPREARKG"
     gene            complement(11822..12334)
                     /gene="tpx"
                     /locus_tag="PL162_02825"
     CDS             complement(11822..12334)
                     /gene="tpx"
                     /locus_tag="PL162_02825"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HQF90999.1"
                     /translation="MERTGIVTMKGNPLTLCGPEIKAGDKAPDFTLLDGKLQPVHLAH
                     FPGKVKILSVTPSLDTPVCDLQAHRFNDEAAQLPGDVVVMNVSMDLPFAINRFCLTGN
                     VDHIKVLSDHRDGAFGLAYGVLIKELRLLARAIFVLDKNDVVRYVELVPEATTPPDYD
                     AALGAARKLL"
     gene            12612..13364
                     /locus_tag="PL162_02830"
     CDS             12612..13364
                     /locus_tag="PL162_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQF91000.1"
                     /translation="MALLVLWVGAGWALSLDDVRHRGVLRIGLEDEPWGFFVRWQEGV
                     PEGLCADLARALAKALGVREEIVAVPWGEGEPGSITGTWQAEDWSRFDVVVSPVAIMA
                     ERQGKVTFSLPYTTVGQMILVRRNGGARAPENLRLGRIGVAQGTSSVPAAQEAFPDAT
                     LVPYDGGEALATPLRQKEIDAVVVDSPVALDFLRRFSDIVALEKLLTRERYALVLPLS
                     ADRSLVEAVNGFLSGEGKAIVDRWSRGDGGKK"
     gene            13490..15787
                     /locus_tag="PL162_02835"
     CDS             13490..15787
                     /locus_tag="PL162_02835"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQF91001.1"
                     /translation="MTLEKAVSLQRRMMTRILPIFFLMAGSLAVAFSFLGFWLRREIL
                     SQVRDETVRSVSEFLGEYGETLSLLENQYTLAMNSILDSLPRIFVADTSAHDVQPMEV
                     TFFARQLFSRLWSDPSSIDGRVFVLTLDPRGKVISSTLPPGLPALPPGARDMLATLSP
                     GGRAVAPLYWDAPSGRYYLYGFSSFPDDSRLGVCLEVNPALPEKILSMIWQIRDVPFV
                     NGVGLYDPADGRPVGRHSPALPPETRRIFSSLKMGEVVPVGGVGRPGESSLFLWSPRR
                     GGHALFGLDRVGVLLQMDLSFFTKIWQGALLGGALFLTVALGLFIGSIARASREVVVP
                     FRALGDQMERFMRDQDAFPSEELRENSWKIEEIRDLERAFLAMAREIVAGLEQLRAAN
                     DELKALYRQQERLTLSLERVFSLASAMADAPDQDEEEFLGQTLRFALELVPEAGAGTA
                     GIVEGGRWHFVAAEGHDRDRLRRLPSMDEQFLPAQEGSVLIPAPLSHLRRAMTDASFE
                     LFQLATLPVYESLVIPLRTGGKPRGHLALDIVDIQKSPFSQESLRVMEALGHLAATFL
                     GMKQLFAVQEGFQREIAMSMISMLEQYDSYTRGHSENVALLAQRLAERLGMGRDAVKN
                     AYWAGLVHDIGKILVPIDVLNKSTSLTEEEYALVKTHPERGERVLSRSEELAGLGLVV
                     GSHHERWDGTGYPAGRRGEEIPPLARVMALADAYDAMTSHRSYRRGMKKSVALEEIRR
                     GAGTQFDPEMAWIFVEMMEGSPEGS"
     gene            16041..16946
                     /locus_tag="PL162_02840"
     CDS             16041..16946
                     /locus_tag="PL162_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HQF91002.1"
                     /translation="MTSGVVFDLHRYSLHDGPGIRTTVFLKGCPLDCWWCHNPESKAF
                     PPELIHRAERCIGCGTCVRVCPREALAPSPPGVWLQRERCDACGRCAEVCPAEALEMA
                     GRSMTVAEVLREVRSDVIFFDESGGGVTFSGGEPLSQPDFLGELLEACGAEGIHRAVD
                     TSGHAPWEVLDRVRRVTDLFLYDLKLMDPHRHRWYTGVDNGLILANLKRLSEEGSRIV
                     LRFPLIPGVNDHRENVDALGALGASLAGVEWVSILPYHAAARDKYKKMGMEYRLSQTL
                     PPSAHMVEQVKGQLEAFGLAVRIGG"
     gene            16952..19312
                     /locus_tag="PL162_02845"
     CDS             16952..19312
                     /locus_tag="PL162_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HQF91003.1"
                     /translation="MNERVTKLRQASVEARPRISIERAVLETEFYQAYEGKHSVPVMR
                     ALNFLHLCEHQTIYIGDGELIVGERGPEPKAVSTFPELTCHSVEDLEILNSRSMANYA
                     VAAEDIVTYREKVIPYWRGRSMRDKAFAEIPQEWKDLYEAGLFTEFMEQRAPGHTTLD
                     GTIYQKGMRDLKKEIAEAQGKLDYLTDPEAVAKNEQLKAMAISCDAIMRFAERHAELA
                     EERASRERDPQRRAELERIAEVCRHVPAHAPRNFWEALQTYWFVHLGTIMELNGWDAM
                     NPGHLDQHLEPFYLKETAAGTLDREGAKELLECFWIKFNNHTAPPKVGVTALESGTYN
                     DFTNINIGGLRRDGTDGSSEVSYLLLEVLDEMHLLQPQANLQLSAKTPDRLLKAACQV
                     IRKGYGYPSVFNADAVVMEQVGMGKAVEDAREGGTSGCIETGCFGKEAYVLTGYLNVP
                     KILEVTLNDGVDPLTGKQVSISTGNPQNFASFDELYAAFAQQLRYVVETKIRVNNYLE
                     RMFATYAPATFLSVVINDCIAKGKDYYDGGPRYNTTYIQCCGIGTVTDSLSALKKHVY
                     EDKTLNLGDLLGALKADFKDREDLRLFLVNKTPCFGNDDDYADSLMQRVYASLLETID
                     GRTSTKGKTYHLDMLSTTCHVYFGKMLGATPNGRKAHLPESDGTSPSHGADRRGPTAV
                     VKSLGKMDQVRSGGTLLNQRFLPSVLEGEEGIAKLGSLIRGYFKLNGHHIQFNVVDSE
                     TLRKAQANPAEHRDLLVRVAGYSDYFVNLDIYHQEEIIARTAQEGF"
     gene            complement(19432..20982)
                     /gene="citF"
                     /locus_tag="PL162_02850"
     CDS             complement(19432..20982)
                     /gene="citF"
                     /locus_tag="PL162_02850"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HQF91004.1"
                     /translation="MSINAVGRHLPDAIPGYGPVKPFQGAAARVPEGHRDGGRLRAVY
                     GGKDKVLPDLKAAIAASGLQSGMTISFHHHLRNGDHVINAVVDACAEMGLRDLVLFPT
                     ALFGVHKKLIPHIKSGVIRRIMGSVNGPIGQLVSEGGMEDPVVLRSHGGRPRAVTSGD
                     VHIDVAFVGAPTADRYGNISGVGSRSACGSLGYAHTDAMYADCVVAVTDNLQPYPISR
                     ISIPQHYVDFVVAVESIGDPAGIVSGSTQITRDPLRLLISRYASQLIEASPCFKNGIS
                     FQTGAGGIPLAVTAFLKDAMKRRNVKGSFGLGGITGYFVDLLREGYVERLLDVQSFDL
                     EAVKSIAENPDHIEISADWYANPWNSGAAVNALDVVILGATELDRDFNANVNTEADGA
                     LLHGTGGHQDTAAGAGLTIIAQPLLRGRIPCVVDAVYSVTTPGEVVDALVTEFGVTLN
                     PRRTDLVEACREAKTVPLVPMEELVDRAKKLTGPMDPVVTEERIVGVIEWRDGTVLDV
                     VRQLTPRK"
     gene            complement(20985..21860)
                     /locus_tag="PL162_02855"
     CDS             complement(20985..21860)
                     /locus_tag="PL162_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HQF91005.1"
                     /translation="MSLRRTMLYLPGNNPNMLLRGHLFRPDGLILDLEDAVAPSEKDA
                     ARILVRDVLCRGEFGGCEVTVRINGMDTELWREDVAAVVPAGATGLRVPKVENPRDIR
                     LLDEYLGEVESQAGIAEGRTAIFCLLETALGIWRAYDIATASPRVAALIPGGEDLTAD
                     LRTNRSADGTELATIRSLLVVAARAAGVDALDTVYPRVADDEGLRREVAFIKQLGFDG
                     KSVIHPNQIPIVHDIFTPTEAEVEKAKRIVAAARDAAARGLGAVQVDGRMVDKPVVRR
                     AEFTLSRAGLSGEVM"
     gene            complement(21857..22129)
                     /locus_tag="PL162_02860"
     CDS             complement(21857..22129)
                     /locus_tag="PL162_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HQF91006.1"
                     /translation="MKTASAGTVESMDCIVTASEADPGSGVTVQIAGASAARFGSAMR
                     AAVLSVVSKMGITDVTLSVQDNGALDLVLQARTEAALERLKGGDVA"
     gene            complement(22236..23492)
                     /locus_tag="PL162_02865"
     CDS             complement(22236..23492)
                     /locus_tag="PL162_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HQF91007.1"
                     /translation="MTRNVMKVREKKDRYQVCYMAGDDSGFDMMEAAMLVLDAMELPI
                     DFVRADLGWAMWEKSVKKYGEGDPRCNTVPPETIEKITNSDATLMAAVTSKSGVKGFK
                     SAILQMRQIFDLYINLRPAKSLPGVGTPLKGDPEIDIVMFRENTEDLYAAVEFNPLPK
                     ELFDVHKGMERFKKCSEVAVSWRVFSREGCERIIRAAFEYAKATGRKTVHNCNKANVI
                     RETDGLMKKIFTEIAKEYEPYGIKGIEENADATAMWLIKNPQDYEVIVASNVFGDILS
                     DEASQLVGGLGFAPSGNIGEKAAIFEPCSGSVPKHAHQYKVNPSAILLTAKMMLEYLD
                     LNEAAAKIEKAVGEVLVEGKVLTYDVWRDFRNDPDWEKKAASTIDMATAIAQKINPSL
                     TGARLEAAKAKAKEMCDWTKPVGFED"
     gene            complement(23599..24093)
                     /locus_tag="PL162_02870"
     CDS             complement(23599..24093)
                     /locus_tag="PL162_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase"
                     /protein_id="HQF91008.1"
                     /translation="MKITGKVWKYGDDVNTDVIFPGKYTYTIKERSEMAKVALEDLDP
                     EFTKNAAPGDIIVGGKNWGCGSSREQAVSCLKERGLGAIIAKGFARIYYRNCLNEGLP
                     IIVSPEAVDAIEAGEDVTVDFDRGKIVTKKGEFAFPPYPDFVRGLIEDGGLIPHVKKS
                     LGLK"
     gene            complement(24096..25352)
                     /locus_tag="PL162_02875"
     CDS             complement(24096..25352)
                     /locus_tag="PL162_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HQF91009.1"
                     /translation="MGKTFAEKALGKAAGYEVRANDVVTVEPHFCMSHDNAAPIAKTF
                     KKIGVKNVWKPERLVFIMDHAIPAPSDEHAVNHKEAREFAKEQGMTHFYDVSSQGGVC
                     HQIMCQEGFALPGIIMIGSDSHTCMYGALGAFSTGIGRSEMAAAWATGKIWFKVPESL
                     KITVTGQFKKGVSAKDLILKIIGDIKADGADYMSVEFHGPAIEAMSLAERMTLCNMGI
                     EMGAKNAVCPPDAKVLEFIKDKAKSDEWEALWADDDAVYAQELSYDLGDLVPCVAKPH
                     AVDNYAPIDEVKGQPIHQAFLGSCTNARIEDLREAAAVLKGHKVAVRTIVIPASWSVY
                     RQAMKEGLLDTFLDAGCVLCNPGCGPCMGNHEGILAPGEVCISTANRNFKGRMGNKDS
                     FIYLASPVTVAASAIAGVFADPREVR"
     gene            complement(25423..25947)
                     /gene="leuD"
                     /locus_tag="PL162_02880"
     CDS             complement(25423..25947)
                     /gene="leuD"
                     /locus_tag="PL162_02880"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163693.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HQF91010.1"
                     /translation="MDQKKLRGRAWVFEDNVDTDLIYHNKYLAETDPKKMPQFAFEYY
                     PGKEKFAKEVKPGDFVVAGRNFGCGSSREHAVYCLKEGGVPVVLAESFARIYYRNAIN
                     NGYPVLFVKGLSQGIKDGKIVDGDELEVDLATGTITNLTQKTSFHGDAVADLENEIMT
                     AGGLIPYLKAQVAQ"
     gene            complement(25978..27258)
                     /locus_tag="PL162_02885"
     CDS             complement(25978..27258)
                     /locus_tag="PL162_02885"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HQF91011.1"
                     /translation="MGKTMIEKIIERASGKQVKVGDRVWCHMDLSTARDFGGANCTEQ
                     FDKVTDKKGLVWDPDKIAFTFDLQAPAHSEGVAKNQKTIRDFAKRQGISKVFDINWGI
                     GQHVLLENGLVKPGDVILGTDSHMNLLGAVGAFATGVGNTDIVASWFKGTNWFRVPES
                     MKITATGTFGKGVCMRDYLTYLVGRLGADGMSFRSVEFDGPVIENANLAERITLCSMV
                     TEMDGKIGLILPNGEVLDWLRSRAGEEVIERTKMITPDADATYVSHMTVDVSSLEPLA
                     SCPDAPDNVKPVREVAGTPINQVHIGSCSNGRFEDIAAAFDILKAGGFTVSPSVRTII
                     TPSTREVMTRCAEAGMIQKFLEAGVVFTNPTCSLCTAEHYGVLPSGDVGVSTTNRNFI
                     GKVGKGSNTYLMSPMSAMAAAVRGVITDPRDLLR"
     gene            complement(27340..27933)
                     /locus_tag="PL162_02890"
     CDS             complement(27340..27933)
                     /locus_tag="PL162_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQF91012.1"
                     /translation="MGNCCCCGGTEGMGSRIEKLLPEIAWITDEALRGQVVATYEDAL
                     KTGGWEPEDMETIPFTLLIPDCPASYLIHTRGVTRMAKVIYEEFNSLYKENGGFQLDH
                     DLLIAGALLHDVGKLVEYEKKDGKVVKSKMGKDLRHPFSGTGLAMRNGVPSRVCHCIA
                     VHAHEGDDGYRCPEAVVINKCDFVNFETIKSFLGLLK"
     gene            complement(28063..28764)
                     /locus_tag="PL162_02895"
     CDS             complement(28063..28764)
                     /locus_tag="PL162_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HQF91013.1"
                     /translation="MVDKRVRQTRIYEKVVDEIKALIASGSLRQGEPVPPERQLMEDL
                     GVSRSSLREAFRVLELLGLIESIPGKGRFVRRGRGEEGGASDPRKLEDGAILELMEAR
                     RVLDPAIAAEAARKARSGDLTRLRRVLSATEGSMAVPKDRAQADFDFHLVLTEATQNF
                     VFVNIVKMTFNLIMATHDRIYSLLSDKDAFLHEHQEIYEAILNHDGEGAARSSSRHIE
                     RIYRTLQEALALEGP"
     gene            complement(29039..29368)
                     /locus_tag="PL162_02900"
     CDS             complement(29039..29368)
                     /locus_tag="PL162_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQF91014.1"
                     /translation="MTDATYEKGVPFRLEELVTVQEKAVVSRTIVDRPTGNATLFSFD
                     KGQALSEHTAPYDALVHVLSGRVEIRLGGVPHTLEPGDALVMPADVPHALKALEPFKM
                     FLVMIRS"
     gene            complement(29442..31088)
                     /gene="hcp"
                     /locus_tag="PL162_02905"
                     /gene_synonym="priS"
     CDS             complement(29442..31088)
                     /gene="hcp"
                     /locus_tag="PL162_02905"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HQF91015.1"
                     /translation="MFCYQCEQTAKGTGCTSFGVCGKDPETAALQDLLVHITGEIGQL
                     AHRARQFNVRDHDVDVFVLEALFTTITNVNFDPARLEGLIKKACDMKSRIHKLYEEAA
                     HKAGKTPDKLCCPSCQESVKDIPSMVRLGEKFSPEADIEKWGPTVGGLRWLVIYGLKG
                     MAAYADHAHILGKESEEAFAFFHEALDGIMADPLTVDALLPLALKLGQKNLHVMELLD
                     AANTSTYGHPVPTPVRIFPKKGKAILVSGHDLLDLHELLVQTEGKGIQIYTHGEMLPA
                     HGYPELHKFPHLAGNYGGAWQDQQKEFDAFPGAILMTTNCIQKPRESYKGRIFTTGLV
                     AWPGVPHVTRKDFSPVIEAALAAPGFPEDGDDKTILVGFARNAVLGAASKVVELVKSG
                     KIRHFFLIGGCDGAKSGRNYYTEFAQKAPKDTLILTLACGKYRFNKLDFGDIEGLPRF
                     LDCGQCNDAYSAIQIAVALAQAFNTDVNSLPLSLILSWYEQKAVCILLTLLSLGIKNI
                     RLGPSLPAFVSPEALQVLVDKFNIQPIGESAEADLKAILG"
     gene            complement(31168..31626)
                     /locus_tag="PL162_02910"
     CDS             complement(31168..31626)
                     /locus_tag="PL162_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HQF91016.1"
                     /translation="MARLIHMSEAASLALHGALLLASPEGRRWTVRELAKEVAASEAH
                     FYKVIQRLAHQGLVRSIRGPGGGVELTRPPETISFLEIYEAVEGPLSPAGCLLGRPSC
                     PVPGCCFDGLLEAASAQIEDYLAHTTLGEYWRRSEGQGGGPGGPGTAEPG"
     gene            31746..33335
                     /locus_tag="PL162_02915"
     CDS             31746..33335
                     /locus_tag="PL162_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HQF91017.1"
                     /translation="MEKSGPFYGWQLAAADRLAGKSGVLAAPTGSGKTWVAYLWAGIL
                     DREGRIHTPPERVIFTAPIKALSNERYLDLRSLGLDVGLETGDFKKNEGASVLCCTQE
                     IYTLKYAGRPGIRLVVDEFHYIFSDSERARTYIDGIRATAPEVPILVMSATFGGPRSV
                     QRYLSRITNRPFVLHESVDRATELIFLERSVRRFGEIHDALIFLFSRDGVTEVADQVA
                     RSRSVLPDEKLRRLDDIAAILGVPQVPLPLMKGVGTYHGSLLPKEKLLVETAFRERLL
                     DVVAGTDALALGVNLPAETVIFAQLAHYHSEDPITKNAFLQMAGRAGRKGHFDPGYVT
                     WLEHSPWENRRFDTGATYRELLRRRPEPARIFLHPAFGRLLRGEVTPEEEAFVVASGS
                     LPEQDFVVSLDDIRRALRKIGTWTKRLVPPHLRRRFREVLADVWFEEMELGQNLALAE
                     LFTAEKRPDALLAAELLERYERNRLQALLKIKRFANALPKGYGFQGMDELGREVERID
                     PTVFTFEERLQEIQESRMGGL"
     gene            33413..34930
                     /gene="pap"
                     /locus_tag="PL162_02920"
     CDS             33413..34930
                     /gene="pap"
                     /locus_tag="PL162_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HQF91018.1"
                     /translation="MLELVDLTKKLAKGEYKELSGRLRDRLGELQRQARDRGIPVLLV
                     FEGWEGAGKGTLMNELILPLDPRGFEVRNLGTPTEEERFYPFLWRYWRWTPARGRLVL
                     FSRSWYRHVFDDRLEGRVTGKKLEEAYGDILNFERQLSTGGALLVKFFLHISAKEQKK
                     RLKTLEDNPATAWRVSPRDWELHARYDERLRVVEEMLHRTDTGHAPWTVVEAHDRDYA
                     VVKVLATVGEALERALKGHDETFRRGDGLRPGGEGEGPPPEEKPFPAGALRPTVLGNV
                     DLSQAISPKVYDGELKELQERFRSAQYALYRKRLPLVVVFEGWDAAGKGGCIKRLTQR
                     LDPRGYQVVPIAAPNDLERSHHYLWRFWTAFPKGGHVAIFDRSWYGRVLVERVEGFAS
                     EEEWRRSYGEINEMELHWCRFGARVLKFWLHIDQKEQLQRFEERRDTPDKAWKITEED
                     WRNREKWPQYEAAVEEMLWRTSTIHAPWTIVEANDKLFARIKVLRTVVEGAESLL"
     gene            35087..36373
                     /locus_tag="PL162_02925"
     CDS             35087..36373
                     /locus_tag="PL162_02925"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF002823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HQF91019.1"
                     /translation="MPNEKALATVRDLRPVAWKAPLRPPAGEVLARHSLGLRDVREAE
                     ARLDRFAPLVRRLFPETKDGIIESPLRSVEAFAALLGEETGRPLQGRFFAKADNMLRI
                     AGSIKARGGIYEVLSVAERIALQTGFLEMGENYARMGDPRGRELFGRYHIAVGSTGNL
                     GISIGTMAAAMGFRATVHMSQDAKQWKKDLLRERGAAVVEYAEDYSKAVEAGRLACHE
                     VPGGHFVDDERSLDLFLGYAVAALRLKGQLDEAGVTVDGAHPLLVYLPCGVGGAPGGI
                     TFGLKLLFGDAVRCFFVEPTHAPCMTLALLLKNPHLSVKDFGIDGITEADGLAVGAPS
                     ALVYDLVGELVDGAYTVEDEPLYRLVELLWSSEGLKAEPSAVSSLWGPLMMGEDRPGV
                     THLAWLTGGLLIPENIFATMRHRGEHGDRLRVPEGL"
     gene            complement(36518..37411)
                     /locus_tag="PL162_02930"
     CDS             complement(36518..37411)
                     /locus_tag="PL162_02930"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HQF91020.1"
                     /translation="MPAPPSPPPQEAPAPPGPRPFRALFLSDLHLGNPWCQARELARF
                     LSFFSCDSLYLVGDIVDGWKARQRSRWPQSHQRVIQKLLQISQHTRICYITGNHDDFL
                     DAFDGRRFGHIEICRRALHHTADGRRFSVLHGDEFDRVVKRHRWIARLGDRAYGVALG
                     VNAGLGALRSWMGWEPWSLSDYLKHKVKDVVGFLSDFEAAVLRETARERSAGVICGHL
                     HRPALKNMRSIVYANCGDWVESCSALAERPDGRLEILRWRDWSAALAPERPFEGEASP
                     EEDPAVATFPLPPAMGGLGLS"
     gene            complement(37604..>39974)
                     /gene="nifJ"
                     /locus_tag="PL162_02935"
     CDS             complement(37604..>39974)
                     /gene="nifJ"
                     /locus_tag="PL162_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HQF91021.1"
                     /translation="FTVGIVDDVTHLSLAVDESFSTEDPKTTRCLFFGLGADGTVGAN
                     KNSIKIIGDDTENYAQGYFVYDSKKSGGITVSHLRFGPKVIRAPYLINRANFVACHQF
                     SFIERLDMLRHAAPESVFLLNSVYGPEEVWDKLPRITQKRILDKKIRFYVIDAYKVAH
                     ETGMGGRINSIMQTCFFAISGVLPREEAIESIKSSIQKTYGKKGEAVVQQNFASVDAA
                     LANLFEVKNPGQVTSTFDLLLPVPAEAPDFVRQTEGPMIILEGDDLPVSSFPVDGTFP
                     SATTQWEKRNIAMECPSWDPELCIQCGKCSLVCPHAVIRGKVYDPALLTGAPETFKST
                     EAKWKQFDGMKYTLQVSVEDCTGCGLCVQNCPAKSKTEEGKKAINMVPQLPLQKTERE
                     NWKFFLSLPEADKGKLNPGTVKDLALYRPLFEFSGACAGCGETPYVKLVSALFGDRAM
                     VANATGCSSIYGGNLPTTPWAKNAEGRGPAWANSLFEDNAEFGLGMRLAVDQQEGFAR
                     ELLGRLDTKLCGSLVKDLLGADQSTDEGIAAQRDRVTKLRECLKGIDLPDARVLEGVA
                     DALVRRSVWIIGGDGWAYDIGYGGLDHVLASGCNVNILVLDTEVYSNTGGQMSKSTPM
                     GAVAKFAAGGKPRGKKDLAMMAMNYGNVYVARVSLGANDAQTVRAFREAESYDGPSII
                     IAYSHCIAHGIDMSQGLEQQKKAVASGHWILMRYDPRRIEEGQNPLQIDCKEPTLPLA
                     DYVYAENRYNILQRSHPEESKALVDCAQEIVKLNWRRYQHMASMSFEKK"
     assembly_gap    39975..40074
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<40075..41033)
                     /locus_tag="PL162_02940"
     CDS             complement(<40075..41033)
                     /locus_tag="PL162_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HQF91022.1"
                     /translation="MSRPKVVVDGNEAAAHVAHKVSEVIAIYPITPSSPMGEWSDEWS
                     SQGRKNIWGTVPQVTEMQSEGGAAGAVHGALQAGALTTTFTASQGLLLMIPNLFKIAG
                     ELTPAVLHVTARTVASHALSIFGDHSDVMACRSTGVGLLASCSVQEVMDLALIAHAAT
                     LEARVPFIHFFDGFRTSHEVAKIEQLTDDDIRALVSDELVAAHRARALNPEHPVIRGT
                     AQNPDVFFQSREACNPFYAACPEVVQRAMDKFAKVVGRSYHLFDYEGDPEAERVIIVM
                     GSGAETVHETVDYLNAQGEKVGVIKVRLYRPFSAEHFLRALPAT"
     gene            41620..42057
                     /locus_tag="PL162_02945"
     CDS             41620..42057
                     /locus_tag="PL162_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQF91023.1"
                     /translation="MVEGQKTVLICDDSMLVRKKLRDSLEKGCGVRVIEAADGAAAVA
                     IYQEERPSLVFMDIVMPLKDGIAALQEIRAFDPQAQVVMASSAGTQDHLRRAIDAGAS
                     DFVQKPFAEDRLREIVQKLCTTVQGGAPCGCPADTSFSEKKDV"
     gene            42050..>42870
                     /locus_tag="PL162_02950"
     CDS             42050..>42870
                     /locus_tag="PL162_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91024.1"
                     /translation="MFSALLGEHLLRERILQPRQLGEVMDRMDRTRLKVGVLAINAGY
                     MTAAQVDEVHRAQTATDRRFGDIAVEKGYLSPLQMDDLLASQRYEHLVMGQALMDMGL
                     MTMARFEEVLQDYKKRYDFDDASFDAAKKGDVDSLMDRLVRVPEGEERRIFVEYLALF
                     ARHLVRFVDRHAALAPEAPSLDYPLPWASYQVIHGPVTLFTALSGTEDLLLRLGEAFA
                     QEPIPAFDDLARASVEECLNQVNGLFTVNMSNRGLDLEMDPPGTSAGARYASRDGG"
BASE COUNT         7321 a        12721 c        14639 g         8089 t
ORIGIN      
        1 ccgctggcca cctcttccac cgaggcggtg atctcctggg tggcggcggc gaggctttcc
       61 atctgggagg atacatcgtc cgcccccgcg cgggactcct cgaccccggc gttggatttc
      121 tccgccaggg cggagaactc ctcggaggtc tcgccgagct tgccggagct gctcaccacc
      181 ttcaccagga attcttttaa tcccgtctcg agggcactgg tgctttccac catcgcggcg
      241 atctcgtttc cctggtaatt cagcaaccac cccttcgacg tgtcgaacct caagtccagg
      301 gcggcgattt ttcgcagcac ctccatgacc gcccccaggg ggcgggtgat ggcccgttcg
      361 gacaaaagcc ccagggccag gcccaagagc accgccccgg cggccacggc cagcaggaag
      421 cgcagggcgg cggcgatatg tttttcgttt tcggcgttgg cctcggcggc ggccttggcg
      481 gcgtgggcgg cgatgctctt gaggttatcg ttaaaggcct ctagcggttt tttggccttg
      541 gccccgtaga gggcgagggc ctcttcgtgg cggttctccc cgaggagctt tatcacatag
      601 tcggtggcgt ggcggtactc gacgaggtgg gtctgggctt ccgccagggt ttttttctca
      661 aagtcgtcca aaggggcctt ggcaaggttt tccaggttct cgttgttcag gagccggcgc
      721 ctctggatgt cttccaaaag ggccccgctc tggcgggcat ccttcgcgag cgccgcttcc
      781 agcatgttcc cccggatggc ctggacgtgg ctccgggcat cgttgaccca ctggatggtc
      841 atcaggcttt tgtcatacat cgtgttcagg ttctcctccg ccgcagtgag ccgggtgtat
      901 cccacccacg aggcgagggc ggtgagggcg caaagcagcg cggtgaggga gagaaggcgc
      961 aggcggatgg tcatgtttct gaagagagcc atcatgtcag atcactccta gcatgttgta
     1021 tgctttcaac acattagagt gttttcgttt tcgaggccgt cggcgtgggg cccatggagg
     1081 gccctgtccc gtaggggcgg ggggggcgag gtccatcgcc ccaccccggg acttccaaag
     1141 aaggtttttt aatccccgat cgtcaccagc tcccgggggg agaggttcat ggattcacag
     1201 cccgtctccg tcaccgccac gatgttttcg atgcggatac cgagctttcc cgggatgtag
     1261 atccccggct cgatgctgaa ggtcatgccg gggcgcaagg gggtggtgtt tcccgccatg
     1321 atattggggg cctcgtggac cgcgatgccg atgccgtgtc ccaggcggtt gtcgaagtgg
     1381 gccccgtacc ccgcgtcgtg gatgatctgc cgagcggctc ggtccacgtc ctgggaaagg
     1441 acccccgggc gcaccgccgc ctccccggcc tcttgggatt tttggacgac ttcgtagatt
     1501 cgccggtcct cggcggtggg ttcccccacg aagaccgtgc gggaggtatc cgaacagtag
     1561 ccgccgcaac gacagccgaa gtcgaggacc acgaagtgac cccgctcgat ggtgctgtcg
     1621 tcggagttgt agtggggcat ggcggcgttg gggcctgtgg cgacgatgga ggagaaggcc
     1681 tcttcgtcgg cgcccttttc gtcgaagagc cgggagatct cccggcggac atcccgttct
     1741 ttcatgcccg gctttatgaa acggaccaga tcggcgaaga cctcgtccgc cagccgtccc
     1801 gcctttctca tgagggccag ttcgtcgggg gccttgcaga tccgcaggtc gtccaggcag
     1861 tgccacccgt tgaccagtcg gacccggtgg cgttcggcga tctcgatggc gtccacggcc
     1921 cggaccccgg agttcatggc cagaggggcc ccttccaggc cgaaatcctt gacggcggcg
     1981 gcaaaggcgc cgtagaacca gttttggtcc acccaactgt agcggggggt gtcttccccc
     2041 agggcgcggg cgaactccgc ctcgtagatc tggggcacga tggcaaagat ctggccttcg
     2101 gcggtgacgc ccacagcctt gaatcgttcg tcgtcaaaga gctccaatcc cgccaggtat
     2161 tccacatccg acgaggggcc catgatgagg gcccgcaggc cgtcctgctt cagtttccgg
     2221 gccaacgcct ccacgcgctc tttctgtagc atgtttatcc ctccgtacca ttcgagatgg
     2281 gatgaccttt cggccccctt gcctccggat ttcccttggg ggcggccacg ggaacggtcg
     2341 cccgggccgc accaggggcg aagcttgtga tatgatctgt cgcaacctcg ctcccttccc
     2401 cgcatgatat gattttatcg ccacctcgcc cccctcgtta agagtcacac gcttcccttc
     2461 tgagggggcg cgagaggggc ctttcgtctc cgcctgcgta ctttatcaca taaacggccc
     2521 tcgcggcggg gggattgggg ttttccccga aagaaataag gggatccggg gtgtttttaa
     2581 gaaataaaag gaggcgaaaa aatctatgag ctcatggcat gaagagctgg cccagggagg
     2641 ggcctgcggg ggtgtggtgt cccttccctg gcgttcttcg gcggatgtga cgttggagct
     2701 gcgccgggga gatcgggtgc ggcggggggt tggggcggag gggtcgccgg ggtggcggct
     2761 ttgtaccacg gaagacggtt tggaggggtg gatccacgag gactttctcg atcctgtggg
     2821 ggaggaagag gccatcctgc gggaggacta ctcctcccgg gaactttcct tggacgaggg
     2881 ggaacgggtg tgggtgcatc gcgccgtggg agggtggctt tgggtccagg tgtcccaggg
     2941 agcctggggg tggatccccg aggatcaggt gaggcccgac tgagcgacca gaagctctcc
     3001 gccccccgaa ggaccgatgg gggggcggag agcttctggc cctgtcagag gatccggaga
     3061 tccaagagct gggtcaggcg ctccagcagg cgcacccccc cgagggagtt tcccttgggg
     3121 tccagggcgg ggccaaaggt ccccaggccc accttcttgg ggaccgaggc cagaatgcct
     3181 ccccccaccc cgctcttggc ggggatgccc acccgtacgg cgaactcccc cgatccgtcg
     3241 taaaggccac aggtggccat gagggcccgg gtcataaggg cgtggcgctc cgacaggacc
     3301 cggtgtcccg aaaggggatt gatgcccccc gaggccaggg tcatacccat caccgccata
     3361 tcccgggcgg tgccccggag ggagcactgg cggaagtagg aatccagcac gttttccacg
     3421 tctccccgaa aggttcccac gctgcgcatg aagtacgcca gggcgcggtt gcggtcgctg
     3481 gtgctttttt ccgaaaggta ggtggcttcg tggtagtcca gggcgtcgtt ccccgagagg
     3541 cgtcgagccc aatcccggac gaaggcgaag cgctcctccg gatcccggtg ggggagcagg
     3601 gagagcacca ccagggctcc ggcgttgatg agggggtttt gggggcggtg gggcttgacc
     3661 atctccagac gcatgatgga attgaagggg tccgccgtgg ggtccatccc cacggcgtcg
     3721 aagacgcgct cttcccccag gagctccagg gcccccgcca gggagatcac cttggaaacg
     3781 gattgcagcg tgaagggaac ggccccatcc cctgcggagg ccacggcgcc gtcgggggtg
     3841 gcgagggcca ctccgagatg gcgagggttt cccttggcga gttcggggat gtaggtggcc
     3901 acttcgccct cgccgggggc ggggcgacag gcgtctaaag cttgctggag cagggtttcg
     3961 gtggggagcg tcatggagtc ctttcctcct gatcgaaagt gggggggcgg aaaccatggg
     4021 gtggcccggc cctcggggaa aattataccc cacggaaggg ggctccggga gctttcatcg
     4081 gggcggggcg cccggggaga atttttctgc acgcagtata tcctcagggg ggattctttc
     4141 ggctataatt gagggaagtg gatgggggcg tttttcttcg aggggaaagc cccccgacct
     4201 tccagagggc gggaggagtg gtcgtggcgt tttccgtgct taccctgtgt ccgggagctc
     4261 agggcacccg gatggcggag ttttgcgatg acgtggcgct ctgggaagaa gaagtgcccc
     4321 acgccccccc ttcgtccgct ccccccgggg gcttgaggga agaggccgag gtgcgatgcg
     4381 aggccctgga gcgctttctt gaggaccatc cccttcgagg gggcgggctc cacgccgtgg
     4441 tggggcgggg gggcgtggtg ggccctgtgc ccgggggatt ttacgaagtg gacgaggcgc
     4501 tggggcgaca cctcctgaag gatcatcccc ggggtcatgt ttccaacctg ggaggggttc
     4561 tggcccgggc cttggcgttg ccccgggggg cccgggccct catgggggac cccctttcca
     4621 cggaggagag ggagcccgag gcccgtctct ccggatggcc cgagctcccc tggcaagccc
     4681 tgggtcatgt ctggagcctc aaggccgccg tgagggccgc cgcccgagat ctgggaaggc
     4741 cctgggagga gctcgccgca gtggtggtgc acctggatag gggcatatcg tcctgtgccc
     4801 accgagaggg gcgcatggtg gacctgataa accccaacga cggaggggcc ttttccctgg
     4861 accaggcggg gggcgtcccc tcgggggacc tggcgcggct ctgtttcagc ggcgcctaca
     4921 ccctacggga cgtgcgcacc gccctggtgg gggcgggggg gctggggggc tacctgggca
     4981 ctagggacct ggaagaagtt cacgcccgaa ggaaggcggg ggacgagggg gcggagcggg
     5041 ccttttccgc catggcctac caggtggggc aggagatcgg cgccctggcg gtggctcttt
     5101 cggggcgggt ggatgcggtg atactcgcgg gtcccctggc gagagatccc ctactggtgc
     5161 agcgcgtgcg ggggtgggtc cagtggatcg ccccggtgct ggtttatccc ggaggggatg
     5221 agctgcactt cctggcggaa ggggcggtgc gagtgctttc tggggaagaa cgggcgaaat
     5281 gctatggaga ctacgctggc atggaggagg aataggttca tgagttggac gtcgttgcag
     5341 tttctgttgg atcggtgccg agacgggcga aagatgcggg tggtggcggt ctgcgcccag
     5401 gatgaggatg tcctcttcgc cctggatcgg gcctcccggg agggcattgt ggatggggtg
     5461 ctcgtgggga gcaaggcgaa gatcgaaggg gcggcttcgg ccctgggcat cagcctcgcc
     5521 ccctttgaga tcgtcgatgc ccccacggag tacgaggcgt cggagcaggc cgtgcggctg
     5581 gtatccagcg gggcggcgga cacgctcatg aagggcatgg tgaagaccgc cacccttctc
     5641 aaggcggtgc tgaacaagga atggggcctc cggtcggggg acctgctgag ccatcttttc
     5701 atggtggaaa ttcccaagct gaatcgggtg gtggggcaca cggacggagg catcaacctc
     5761 tatccggatc tcaagacgaa agtgggcatc ctgaataacg ccgtggactg ctatcacaag
     5821 ctcggggtgg cctgccccaa ggtggcggtc ctcggggcgg tggaagtggt gaatccagac
     5881 atgccatgca cggtggacgc agcggccctg gtgcagatga accggcgggg gcagatcaag
     5941 gggtgcctgg tggatggccc cctggcgctg gacaacgcgg tgagcctcga ggcggccaag
     6001 cacaagggca tcgtctccga cgtggcgggg tgtgcggaca tcttcatggt gccgaacatc
     6061 gaggcgggaa acctgctggg caaggtcatg ctctacatga ccggcggcgt gggggcgggg
     6121 gtcatcctgg gggcccaaaa gcccgtgatc ctcaccagcc gcttcgattc cgccgagacc
     6181 aagctctatt ccatcgccct cggcgcggtg ttgtcccaga aatagctccg ggactttcca
     6241 tgggagtttt tcggacccgg ggttcgccgg acgagacccg gagtctaatt ttcaaaacta
     6301 tctgaatttt ttgaggaggc gggaagagaa aatggagcag ttgcggtcac tgacggcgtt
     6361 gatggagtac gcgaagcagg tgggaccccg gaagatcagt gtggccatgg ccgaggatgc
     6421 ggaggtcctg gaggcggtgg aagaggcccg taaagcgggg gtggccaacg ccgtcctggt
     6481 gggtaacggg gacaagatcg ccgaaatcgc gggcaagctc ggcatcgacc tgggccacta
     6541 cgaggtcgtg gatgagcggg gcggcgagcc cgccgtggcc ctggcggcgg tggagctcgt
     6601 gtcctccggt aaggcggaga tcctcatgaa gggcatgatc aagaccgcca atttcctgcg
     6661 gggcgtgctc cataaggaaa aggggctccg cacgggatcc ctcatttccc acgtctacgt
     6721 gcaccatgtg gaggggtacg accggatatt tttcatctgc gaccccgcct ttaacatgta
     6781 ccccgatctg ggggaaaagg tgaaaattct ggagaacacc gttcgcctgg cccatgcctt
     6841 tggggtggaa aaacccaagg tggcggtgtt ggcggcggtg gaagtggtga accccgacat
     6901 gccctgcacc ctggatgcgg cggcgattgc ccagatgaac cggagggggc agatcaaggg
     6961 atgcatcgtg gatggcccct ttgccctgga taacgccatc agcgaggagt cggcggcgca
     7021 caagggcatc gtctccgagg tggcgggcaa ggccgacatt ctcctggtgc cggacatcga
     7081 cgcgggaaac atgatggcca aggccatcgt ctacatgacg aacaacaaga cggcgggact
     7141 ggtgctggga gccaaggccc ccgtggtgct cacgagccgg tcggacagcg ccgagaccaa
     7201 gctcctgtcc atcgcctccg ccgtggccct ggcggcatat aagggaaact aatctccgga
     7261 gacctttctg cacgagggcg cctcgccgct tgagaccact cccttgccgg acggcggtcc
     7321 tcgaagatcg gaaccggagg gagaaatccc ggaattttca ggacgaagga tggaaaatgg
     7381 tgagaaggga aaagcgggag ctctttcgga gcacgctccg aaagagctcc cggaactcct
     7441 ttcctgcacg tttttcatcc tttttttctc ctggaccttc gttgaagagg agtgttcccc
     7501 ccatggatta cgcaattttt gccatcaatc ccggttccac cagtaccaag gtggcgtttt
     7561 tccggggagc ggaagaaatt tggagcgacg tacagcgctt tgacgtggag acgctgcgag
     7621 ccttcgccac cgttgccgac caggaggact tccgcctgga agagattcgc aaggccatgg
     7681 aacgtcacca tgtcaccgaa aaagacctgg acgcagtggt gggtcggggg gggttgatca
     7741 agcccattcc cgggggtacc tatgaggttt ccgatgccat ggtggagacc ctgcggtccc
     7801 aaaagtacgg tgcccacgcc tcgaacctgg gggccccctt ggccatgagg gtcgcccagg
     7861 gcaagcccgc cttcatcgtg gacccggtgg tggtggacga gctgatcccc gaggctcgcc
     7921 tttcggggat gccccagatc gagcgccggt ccatcttcca cgccctcaac cagaaggccg
     7981 tggggcgacg ggcggcggcg gacctgggaa aaccctacga gaaggccaac ttcgtcatcg
     8041 cccacctggg agggggcatt tccgtggggg cccactgcca ggggcgggtg atcgacgtaa
     8101 ataacgccct ggacggggac ggcccttttt cgccggagcg agccgggggg ttgcccgcag
     8161 gggacctggc gcggctctgc ttcagcggcg cctatgacct gacggaagtt ataaagaaga
     8221 tggtgggggg cgggggcatg gtggcccatc tgggcaccaa cgacctccgg gaggcccagc
     8281 gggccgccga aggaggagac cccaaggcga cgctggtgct ggacgccatg gcctatggag
     8341 tggccaaaga aatcggatcc cgggcggtgg cgctcgcggg gaaggtggat gcggtcatcc
     8401 tcaccggggg attggcctac agcgagggct tcgtacgccg catcggagac cgggtatcgt
     8461 ggatcgcgcc ggtgaaggtc tatcccggtg aggacgagct ccaggcgctg gcggaggggg
     8521 ccctgcgggt gctctccggc caggaggctc cccgggttta cgagggctag ggccgccggt
     8581 agagctcttt tgcctgggaa atctcggaag gagagggaag gggatccttc cctctcctat
     8641 ttttattttt taagtttacc ccttgcgggc ttctcttgga gtttttttgg cctttttttc
     8701 aggggatcgc cggggggcgc gccggtccag ggcctgccgc agggcctggt gcagggtttc
     8761 ggcggtgaag ggcttaggga taaagccgtc ccctccggcg tcaaaccagc gcccggagtc
     8821 ctccggtcgg gcggaggccc ccagggccac cagggatagg gaggggtagc gctggtcccg
     8881 gagggaaagg gcgtctttca ggcatcccag ctccccctcg ggcatgtcca aaatcaccag
     8941 gtcaaagggc tcccgttcca gcatttccgt cgcctgatcc ccgctctccg ccacgcgaag
     9001 ttctgctttg tccttgcgaa gcatgagctg gatcagcttc cggtctacgg ggttgtcctc
     9061 caccaaaagg acccggaggc ccgggggcaa gggaaatcct ccggaagggc cggggccgga
     9121 ggcgggggga aggttcaggg agaaggagaa ggtgcttccc tttcccggga cgctctccac
     9181 ggagaccgac cctcccaggg cttggacgat cctcttcgtg atggcgagcc ccgtgcccgc
     9241 cccttggtgc ctcttgctca ggccatcgtc cagctgggtg aagggttcga agattttttt
     9301 gaggtcctgg gcggcgatgc ctatcccgga atcggtcacg gagaaccggt aaagcgaggg
     9361 gtttccgccg ggatcttccg gggatatcgc cagggagacc cccccttcgg aggtaaaaga
     9421 tgccgcattg tgcaggagta cgtccaggca ggtgcgcagg agtccctcgt cccctaggac
     9481 tttttccggc aggcgcggat cccagaggac gcggaaggtc agccccttgc gccgaaagag
     9541 ctggcccgtc tcagaccccg ctttctccac cagggcccgg agatccagcg gccccggggt
     9601 cggggagatt ccctcggtct ccagccgggc cagggtgagg agattattca gcagggacag
     9661 gagtttttcc ccggaggaca ggatgtgctg caacagatcc tgttggtcct ccgacaggag
     9721 ggaatccttc aggagacgcg ccatgcccag gaccgcattc aggggggtcc gaatttcgtg
     9781 gctcatgttc gccaggaaga cgctcttggc ccgattggcc ccttcggcct ggtcccgggc
     9841 cgcccggata gattcttcga tctccttgcg atgggtgatg tctatggcca ggcaaaccac
     9901 ccgatcgcag aggtgatccc cccgacagag gggagccagg cgggcttcga agctccgtcc
     9961 cccttcgtcg gtgtactcca cggtctggag gctcaggtgg gaaagggttc gcccgatggc
    10021 ctcccggacc cgggggaccg tctcgggggg gaggaagtcc tcgagcttgt gccccgaagg
    10081 aaatctcggg agttggtccg gcgccatgtg ggtcaccacg tccgtgacgg tgccgccttg
    10141 gtcaaagacg aggaccgcgt ccggtacggt gcgaaggatg gcctccgtgt accggatctg
    10201 ggccttcaag gcgtcttcgg tcttcttgcg ccgagcgttt tcctggcgta gttccctgtt
    10261 ggtcgtctcc aactcggcgg tgcgctgctc caccctctgc tcgaggaggt cccgggactc
    10321 ccggagggcc tgttcggtct tcatggcctc ggtgatatcc cgccccacgc cctggatctc
    10381 caggagcgat ccccggtcgt cgaagatccc cccgtaactc cattggagcc agcgcgtctc
    10441 tccccctcgg gtgatcatgc gctccgtgcg gaggagccgg gggcttttca ggacgatccg
    10501 ggccatggcc tcccgaaggg gctcctgggc cgagggaagg gcgcactgga agacggaaaa
    10561 agcgtggagg gcgtggcgat ccagcccaaa aaaggagaaa aaggcccggt tggcgaagcg
    10621 aagaaggcca tcgggggaaa aacggcacac caggtcgtcc tggtcctgga ccaccgcctc
    10681 gtagcgcttc tcgctctggc gcagggcccg ctcggtggcg cgcttcttca gggcgtgctc
    10741 caggaggtcg cggaagaact tcagctcttc cacgtcgtcc tggctccagg cccccggatg
    10801 gcggttgtta tcgaagccca cataccccgt caccgagtcc cccgatcccc agctcagcac
    10861 gaagagggat tggatgccct gggcccgaag gatttcccgt tcatgggccg cctcgggggg
    10921 catggagcgg acgtcgggga cgatgatggc tccctcgcgc cggagggtgt cgatccacca
    10981 cggagtggca tccatggcaa tgcgctgcag ctggtctttt gccggggata tgccgggggc
    11041 gcaccattcg tgggtgttgc tgtgggtggc cccctcgggg tcgacctgga agacgtaccc
    11101 ccggtcggcg ctgaaaaagg cccccagatc tcccagggcc tgatggatgg cgtggtccag
    11161 acagaggtcc tttaggaatc gggccgaaat gcgcgaaagg accttctcca ggaccaggcg
    11221 ccgccggagg gagtcctcgg cgaggcacag gctggagatg tcctggtagg cggcgtaatg
    11281 aaaggccccc cgggacgtct tgatgggaaa gaccgtcagg gccacgggca atcgggagcc
    11341 gtcctttcgg ctccggaacg tggaggaaag ggagacgtat tccccggcgc ccacccgccg
    11401 agagaggtct tccgcctcgg ctagctcctc ggaggaggtg gtcaccaggt gatcgatgat
    11461 cttcccccgg atctcttcgt cggagtattg aaacatctcc cgaaattggc ggttcgaccg
    11521 gatcacccgg cgatgggcgt cgacgaagac gatgccctcg gaggcctcct cgaagagctc
    11581 ctcgaagaag gccccgacga tggcggaatc ccgcagggtg tcctccgggg cggaggcgtc
    11641 ggcatggacg tcttcaggga gggggggagt gggggataga tcttcgccgc cgggcatgcg
    11701 tccgggcctc ctttgttgtc atgatttgtc tttaggtcat aacagatcca tcaccccggg
    11761 cgcgacgccc cgggcccttc gcttttcggg ggggcgtcaa ggtcgtcagg aagaaaggag
    11821 cctaaaggag ctttcgggcg gcccccaggg cggcgtcgta gtccggaggc gtggtggcct
    11881 cggggacgag ctccacgtag cgcaccacgt cgtttttatc cagcacgaag atggcccggg
    11941 ccaggaggcg gagctctttg atcaggacgc cataggccag gccgaaggcc ccgtctcggt
    12001 gatcggagag caccttgatg tggtccacgt tgcccgtcag gcagaaccgg ttgatggcaa
    12061 aggggaggtc catgctgaca ttcatcacca ccacgtcgcc ggggagttgg gccgcttcgt
    12121 cgttgaaacg atgggcctga aggtcgcaca cgggggtgtc cagggagggg gtgacgctca
    12181 agatcttcac cttcccggga aagtgggcca agtgtacggg ttggagcttg ccgtccagaa
    12241 gggtgaaatc tggggccttg tcgcctgctt tgatctcggg gccacacagg gtcagggggt
    12301 tgcctttcat ggtgacgatg ccggtccgtt ccatggggag aacacctccg taggtcgtga
    12361 aagatttttt tccacgggtc tcttggatcg ataagattat acgccatggt cgccttttat
    12421 agaatgggag gggggataaa attctccctt gggggggcat acacgggggc tccgatgcgc
    12481 tatactggac gggagagata aagacgtctt agagatggag cctcggcccg gacgggtctg
    12541 cggaagcgac ggcgaggcgg tatgcggggg gtggcatggt gggtcgatgg ggggcgcgag
    12601 gagcgtgggc ggtggccctt ttggtccttt gggtcggcgc gggatgggcc ctttccttgg
    12661 atgacgtgcg ccaccggggg gtgctccgca taggtttaga ggacgagccc tggggatttt
    12721 tcgtgagatg gcaggagggg gttccggagg gtctttgtgc cgacctggcc cgggccttgg
    12781 cgaaggccct cggcgtcagg gaagagatcg tggcggtccc ctggggagaa ggagagcccg
    12841 ggagcatcac gggcacctgg caggcggagg attggtcccg tttcgacgtg gtggtgtctc
    12901 cggtggccat catggcggag cgccagggga aggtgacgtt ctccctcccc tataccacgg
    12961 tggggcagat gatcctggtg cggcggaatg ggggggcccg ggctccggaa aacctccgcc
    13021 tcgggaggat cggagtggcc caaggcactt ccagcgtgcc cgccgcccaa gaggccttcc
    13081 ccgacgccac gctggtaccc tacgacggag gtgaagccct ggcgactccc ctgaggcaaa
    13141 aggagattga cgccgtggtg gtggacagcc ccgtggctct ggactttttg cgccgtttct
    13201 ccgacatcgt cgcactggaa aagctcctca cccgggagcg ctacgccctg gtgctccccc
    13261 tttcggcgga ccgatcgctg gtggaggcgg tgaacggctt tctgagcggc gaggggaagg
    13321 ccatcgtcga ccgatggagc cggggcgatg ggggaaagaa gtaggcccct tcggccggga
    13381 gggtggaaga cggatcgccg gtggatgtga gggcgaaagc cagggcccct tcgtgggagt
    13441 gagccgatgg ggggcccgag aagaagaaga gttcgaaaga gtcggggcga tgacgttgga
    13501 aaaggccgtt tccctacagc gccggatgat gacgcggatc ctgccgatct ttttcctgat
    13561 ggcggggtct ttggcggtgg ccttttcgtt cttgggattc tggctccgga gagagatcct
    13621 ctcccaagtc cgagatgaga cggtgcggtc ggtctcggag tttttggggg agtacggcga
    13681 gaccctttcc ctcctggaaa accagtacac cctggccatg aacagcatcc tcgacagtct
    13741 gccccggatc ttcgtcgccg acacctccgc ccacgacgtc caacccatgg aagtgacgtt
    13801 ttttgcccga cagctctttt cccgcctctg gagcgaccct tcttcgatcg atgggcgggt
    13861 cttcgtgctg actctggacc ctcgaggaaa ggtgatctct tccaccttgc ctccgggact
    13921 tcctgccctg cctccggggg cccgggacat gttggcgacg ctgtcccccg gggggcgggc
    13981 tgtggcgccc ctttactggg acgccccttc gggacggtat tatctttatg gtttttcctc
    14041 ctttcccgac gattcccgcc tgggggtctg tctggaggtc aatcccgccc tgccggaaaa
    14101 aattctctcc atgatatggc aaattcggga tgtgcccttc gtgaacggcg tggggctcta
    14161 cgaccccgcc gacgggcgcc ccgtggggcg ccactccccg gcccttcccc ccgagacgcg
    14221 gcgaatcttc tcttcgttga agatggggga agtggtcccc gtcggagggg tggggcgccc
    14281 gggggagagc tcgctctttc tctggagccc ccgacgaggg ggacacgccc tttttgggct
    14341 agatcgggtg ggggtgctcc ttcagatgga cctctccttc ttcaccaaga tctggcaggg
    14401 ggccctcctg ggaggggccc tttttctgac ggtggccctg gggcttttca tcgggagcat
    14461 tgcccgggcc agccgggaag tggtggtccc ctttcgggcc ctgggggatc agatggagcg
    14521 ctttatgagg gatcaagacg ccttcccttc ggaggaactt cgggaaaact cgtggaaaat
    14581 cgaggagatc cgagacctgg agagggcctt tttggccatg gcccgggaga ttgtggcggg
    14641 gctggagcag ttacgggccg ccaacgacga actgaaggcc ctgtaccgcc agcaggaaag
    14701 gttgacgttg agcctggagc gcgtcttctc cctggcgtcg gccatggccg atgcccccga
    14761 tcaggacgag gaggagtttc tcggccaaac actgcgcttt gccctggagc tggtgcccga
    14821 ggccggggcg ggtacggcgg ggatcgtgga aggggggcgg tggcacttcg tggccgccga
    14881 gggccacgac cgcgaccggc tacgccgtct gccctccatg gacgaacaat tcctccccgc
    14941 ccaggagggg agcgtgctca ttccggcgcc cctgtcgcac ctgcgccggg ccatgaccga
    15001 cgcttccttc gagctttttc agctcgccac cctgcccgtt tatgaatccc tcgtgatacc
    15061 cctgcgtacc ggggggaagc cccggggaca tttggccctg gatatcgtcg acatccaaaa
    15121 aagccctttc tcccaggagt ccctccgggt catggaggcc ctgggtcact tggcggccac
    15181 gttcctcggc atgaagcagc tctttgccgt gcaggagggc ttccagaggg agatcgccat
    15241 gtccatgatc tccatgctgg agcagtacga tagctatacc cgagggcatt cggagaacgt
    15301 ggccctgctg gcccagcgcc tggcggagcg gttggggatg gggcgggatg ccgtgaaaaa
    15361 cgcctattgg gcggggttgg tccacgacat cggcaagatc ctggtgccca tcgatgtcct
    15421 caataagagc acctctctga cggaggaaga gtacgccctg gtcaagaccc accccgaacg
    15481 gggagagcgg gtcctatccc gctccgaaga gttggcaggg ctgggtttgg tggtgggaag
    15541 ccaccacgaa agatgggatg gcacgggata tcccgcaggg cgtcgagggg aagaaattcc
    15601 tcccctggcc cgggtgatgg ccctggcgga cgcctacgac gccatgacca gccaccgatc
    15661 ctaccgccga ggcatgaaaa aaagcgtggc cctggaggag attcgacgag gggcggggac
    15721 gcagttcgac cccgagatgg cctggatctt cgtggagatg atggagggat cgccggaagg
    15781 ctcctagctt tcccggaggg atccggttag ggggaaatct ccccatgagg gggcggggca
    15841 tgacacgatt tctaacaaaa aagatcttta gtttcccccc caaaggcctt gacgaaccct
    15901 ccggggcgtg atattttacc gcgtaagaga gctccgtcct cccccacgca aaggttccag
    15961 agttgccgcc gggaggtttt ttttcagact cgcggactgt attcaaagaa tacactcatc
    16021 gaaggaagag agggagatcc atgacgtcgg gcgtggtttt cgaccttcac cgctattccc
    16081 tccacgatgg cccggggatt cgcaccaccg tcttcctcaa ggggtgtccc ctggactgct
    16141 ggtggtgcca taaccccgag agcaaggctt ttccgccgga gctcatccac cgggcggagc
    16201 ggtgcatcgg ctgcggcacc tgcgttcggg tctgtccccg ggaggccttg gccccgtctc
    16261 ccccgggggt gtggctccag agagagcgct gtgacgcctg cggtaggtgc gccgaagtct
    16321 gtcccgccga ggccctggag atggcggggc gctccatgac ggtggcggag gtcctgcggg
    16381 aggtgcgctc cgacgtgatc tttttcgacg agtcgggggg aggggtgacc ttttcggggg
    16441 gcgagcccct gagccagccg gactttctcg gggagctcct ggaggcctgc ggcgccgagg
    16501 gcatccaccg ggcggtggat acgtcggggc acgccccctg ggaggtgctg gatcgggtga
    16561 ggcgcgtcac ggatcttttc ctctacgacc tgaagctcat ggatccccat cgtcatcgct
    16621 ggtatacggg ggtggacaat ggccttatct tggccaacct taagcgcctt tccgaggagg
    16681 ggagccggat cgtcctgcgt tttcccctga tccccggcgt caacgaccac cgggaaaatg
    16741 tcgacgccct gggggccctg ggagcctccc tggccggggt ggagtgggtg agcatcttgc
    16801 cctaccacgc cgccgcccga gataagtaca aaaagatggg gatggaatac cgcctttccc
    16861 agacgttacc cccctcggcc catatggtgg agcaggtcaa ggggcagttg gaggccttcg
    16921 gccttgcggt gaggatagga ggatgatcgg tatgaacgaa cgcgtaacga agctgcgtca
    16981 ggccagcgtg gaggcccgtc cccggatctc catcgagcgg gcggtgttgg agaccgagtt
    17041 ttaccaggcc tacgagggga aacattccgt tcctgtgatg agggccctga acttccttca
    17101 cctctgcgag catcagacca tctatatcgg cgatggggag ctcatcgtgg gagagcgggg
    17161 tcccgagccc aaggccgtgt ccacctttcc cgagctcact tgccattccg tggaggactt
    17221 ggagatcctc aactctcgga gcatggcgaa ctacgccgtg gcggcggagg acatcgtcac
    17281 ctatcgggaa aaggtcatcc cctactggcg ggggcgtagc atgcgggaca aggcctttgc
    17341 cgagatcccc caggagtgga aggacctcta cgaggcgggg cttttcacgg agttcatgga
    17401 gcagcgcgcc ccggggcaca ccacgctgga cggcaccatc taccaaaagg gcatgcggga
    17461 cctgaaaaaa gagatcgccg aggcccaggg aaagctcgat tacctaaccg atcctgaggc
    17521 cgtggcaaaa aacgagcagc tcaaggccat ggccatctcc tgcgacgcca tcatgcggtt
    17581 cgccgagcgc cacgccgagc tggccgagga gcgggcctcc cgcgagaggg acccccagcg
    17641 ccgggcggag ctcgagcgca tcgcagaggt atgccgccac gtccctgccc acgccccccg
    17701 gaacttctgg gaggccctcc agacctactg gttcgtccat ctgggcacca tcatggagct
    17761 caacggctgg gacgccatga atccggggca tttggatcag catctggagc ccttctacct
    17821 gaaggagacg gcggcgggaa cgctggaccg ggagggggcc aaggagctcc tggagtgctt
    17881 ctggatcaag ttcaacaacc acaccgcccc ccccaaggtg ggcgtcacgg ccctggaaag
    17941 cggcacgtac aacgatttca ccaacatcaa catcggcgga ctgcgccgcg acggcaccga
    18001 cggttccagc gaggtctcct atctcctgct ggaagtgctg gacgaaatgc acctcctcca
    18061 gccccaggcg aacctccagc tcagcgccaa gacccccgat cggctcctaa aggccgcttg
    18121 ccaagtcatc cgcaagggct acggctatcc ttccgtcttc aacgccgatg cggtggtcat
    18181 ggagcaggtg ggtatgggca aggccgtgga agacgcccgg gaagggggca ccagcggctg
    18241 catcgagacc gggtgcttcg gcaaggaagc ctatgtgctc acggggtacc tcaacgtgcc
    18301 caagatcctt gaagtgaccc tgaatgacgg cgtagatccc ctcacgggca agcaggtgag
    18361 catctccacg gggaaccccc aaaacttcgc ctccttcgac gagctctacg ccgcctttgc
    18421 ccagcagctc cggtacgtgg tggagacgaa aatccgggtg aacaactatc tggaacggat
    18481 gtttgccacc tatgcccccg ccaccttcct gtcggtggtc atcaacgact gcatcgccaa
    18541 gggcaaggat tactacgacg gggggccccg gtataacacc acctacattc agtgctgcgg
    18601 catcggcacc gtgacggaca gcctttcggc gctcaagaaa cacgtatatg aagataaaac
    18661 tttgaatctc ggggatcttc tgggggcctt gaaggccgat ttcaaagatc gggaggatct
    18721 gcgcctcttc ctggtcaata agaccccctg tttcggcaac gacgatgact acgccgattc
    18781 cctcatgcag cgggtctacg cctcgctgct agagactatc gacggccgca cgagcaccaa
    18841 gggaaagacc taccacttgg atatgctatc caccacgtgc cacgtgtatt tcggcaagat
    18901 gctcggggcc actcccaacg gccggaaggc ccatctgccc gaatccgacg gcacctcccc
    18961 ctcccacggg gcggatcgcc ggggccccac ggcggtggtc aagtccctgg ggaaaatgga
    19021 tcaggtgcgc tccggcggca cgctgctgaa ccagcgtttt ctcccctcgg tgctcgaggg
    19081 ggaagagggg attgccaagc tggggagcct catccgggga tatttcaagc tcaacggcca
    19141 tcacatccag ttcaacgtgg tggattccga gaccctgcgc aaggcccagg caaacccggc
    19201 ggagcatcga gatctgttgg tgcgcgtggc ggggtacagc gactacttcg tcaacctgga
    19261 catctaccac caggaagaga tcatcgcccg caccgcccag gaagggttct agacctcaag
    19321 atcgcagatc tccagaggct ttgaggcgac atcaccaaag gggagctccc gaaaagaccg
    19381 ggagctcccc tcctttatgt ttatggtgga ttttttggaa aaacagcgcg attacttccg
    19441 gggcgtgagc tggcgcacca cgtcgagcac cgtgccgtcc cgccactcga tgacccccac
    19501 gatgcgctcc tccgtgacca cggggtccat gggccccgtg agttttttcg cccgatccac
    19561 cagctcctcc atggggacca ggggcacggt cttcgcctcc cggcaggcct ccaccaggtc
    19621 ggtgcgccgg ggattgaggg tgacgccgaa ctccgtcacc agggcgtcca ccacctctcc
    19681 cggggtggtg acggagtaga ccgcgtccac cacgcagggg atgcgccccc ggagcagggg
    19741 ttgggcaatg atggtgagcc ccgcccccgc cgccgtgtcc tggtgccctc ccgttccgtg
    19801 gagcagggcg ccgtcggctt cggtgttcac gttggcgttg aagtcccggt ctagctccgt
    19861 ggcccccaaa atcaccacat ccagggcgtt caccgcagcc cccgagttcc agggattggc
    19921 gtaccagtcc gccgagattt cgatgtggtc cggattttcg gctatggact tcacggcctc
    19981 cagatcgaag gattggacgt ccaggagacg ttccacgtac ccttcccgca gcagatccac
    20041 gaagtacccc gtgatgcctc cgaggccgaa gcttcccttg acgttccgcc gtttcatggc
    20101 gtccttcaga aaagccgtga cggcgagggg gatgcccccc gccccggtct gaaaggaaat
    20161 gccgtttttg aaacaggggc tcgcctcgat gagctgggag gcgtagcggg agatgagaag
    20221 gcgtagcggg tcccgggtga tctgggtgct tccggagacg atgcccgccg ggtccccgat
    20281 gctttccacc gccacgacga agtccacgta gtgctgaggg atggagatgc ggctgatggg
    20341 atagggctgg aggttgtccg tcaccgccac cacgcagtcg gcgtacatgg cgtcggtgtg
    20401 ggcgtacccc agggagccgc aggccgagcg cgatcccacg ccggagatat tgccgtaccg
    20461 gtccgccgtg ggggccccca cgaaggccac gtcgatgtgc acgtcgccgc tggtcaccgc
    20521 ccggggtcgt cccccatggc tccggagcac cacggggtct tccatgcctc cttcggagac
    20581 gagctggccg atggggccgt tcaccgaccc catgatgcgg cggatgacgc cgcttttgat
    20641 gtggggaatg agctttttgt gcacgccgaa gagggcggtg ggaaagagta ccagatcccg
    20701 gagccccatt tcggcgcagg cgtccaccac ggcgttgatg acgtggtctc cgttgcggag
    20761 gtggtggtga aaggaaatgg tcatgccgct ttggagcccc gaggcggcga tggcggcctt
    20821 gaggtcgggg agaaccttgt cttttccccc gtagacggcc cggaggcgtc ccccgtcccg
    20881 gtggccttcg ggcacccggg cggcggcccc ctggaagggc ttcaccgggc cgtatcccgg
    20941 gatggcatcg ggaagatggc gtcccacggc gttgatggac atgatcacat cacctccccg
    21001 gaaagtcccg cccgcgatag ggtgaactcc gcccggcgta ccacgggctt gtccaccatg
    21061 cggccgtcca cctggacggc cccaagaccc cgggcggcgg cgtcccgggc ggcggccacg
    21121 atgcgcttgg ccttctccac ctcggcctcg gtgggagtga agatgtcatg gacgatggga
    21181 atctggttgg ggtggatgac gcttttgccg tcgaagccca gctgcttgat gaaggccacc
    21241 tcccggcgga gtccttcgtc gtcggcgacc cgggggtaga ccgtgtccag ggcgtccact
    21301 ccggcggctc gggcggccac caccagcagg ctccggatgg tggcgagctc cgtgccgtcg
    21361 gcggagcggt tggtgcgcag atccgccgtg aggtcttctc ccccggggat gagggccgcg
    21421 acccgggggg aggcggtggc gatgtcatag gcccgccaga tgcccagggc cgtttccagg
    21481 agacagaaga tggcggtgcg tccctcggcg atccccgcct gggattccac ctcgcccagg
    21541 tactcgtcca gcaatcggat gtcccggggg ttttccacct tgggaactcg gaggcccgtg
    21601 gctccggcgg gcaccacggc ggccacgtct tccctccaaa gctccgtgtc catgccgttg
    21661 atgcgcacgg tgacctcgca ccccccgaac tctccccggc aaagcacgtc ccggacgagg
    21721 atccgagcgg cgtccttttc cgagggggcc acagcgtctt cgagatcgag gatgaggccg
    21781 tcggggcgga agaggtgtcc ccggaggagc atgttggggt tgttgccggg gaggtagagc
    21841 atggtgcggc ggaggctcat gcgacatcac cccccttgag gcgctccaag gccgcctcgg
    21901 tgcgggcctg gaggaccaga tccagggcgc cgttgtcctg cacggagagg gtgacgtcgg
    21961 tgatgcccat cttcgaaacc acagataaga cggcggcgcg catggcgctg ccgaaccggg
    22021 cggcgctggc cccggcgatc tgtaccgtga cgccgcttcc cgggtcggcc tcactggccg
    22081 tgacgatgca gtccatggac tccaccgtgc cggcagatgc ggttttcatg gcaaacctcc
    22141 ttttcaagag agagcacgaa atagagggga aaaggggcgc aaagggaaga gatccccttg
    22201 cgcccgaaga agttcccgaa gtcccgggac gcgagctaat cctcgaagcc cacgggcttc
    22261 gtccagtcgc acatctcctt ggccttggcc ttggcggcct cgaggcgggc tccggtgagg
    22321 gaggggttga tcttctgggc gatggcggtg gccatgtcga tggtggaagc ggccttcttc
    22381 tcccagtcgg ggtcgttgcg gaagtcgcgc cacacgtcgt aggtgaggac cttgccctcc
    22441 acgagcactt cgccgacggc cttttcgatc ttggctgccg cctcgttcag gtcgaggtac
    22501 tccagcatca tcttggcggt gagaagaatg gccgaggggt tcaccttgta ctggtgggcg
    22561 tgcttgggca cggagccgct gcagggttcg aagatggcgg ccttttctcc gatgttcccg
    22621 ctgggggcaa agcccaggcc gcccacgagc tggctcgcct cgtcggagag gatgtctccg
    22681 aagacgttgc tagccacgat gacctcgtag tcctgggggt ttttgatgag ccacatggcg
    22741 gtggcgtcgg cgttctcctc gatgcccttg atgccgtagg gttcatattc cttggcgatc
    22801 tcggtgaaga tcttcttcat gagaccgtcg gtttcccgga tgacgttggc tttgttgcag
    22861 ttgtgcacgg tcttgcgccc cgtggccttg gcgtactcga aggccgcccg gatgatgcgc
    22921 tcacacccct cccgggagaa gacccgccag gacacggcca cttcggagca cttcttgaaa
    22981 cgttccatgc ctttatggac gtcgaagagc tccttgggga gggggttgaa ctccacggcg
    23041 gcgtagagat cttcggtgtt ctcccggaac atgacgatgt cgatctcggg atcgcccttg
    23101 agcggcgtgc ccaccccagg gaggctcttg gcggggcgca ggttgatgta gaggtcgaag
    23161 atctgccgca tctggaggat ggcggacttg aagcccttca cgccggactt ggaggtcacg
    23221 gcggccatga gggtggcgtc ggaattggtg atcttctcga tggtctcggg gggcacggtg
    23281 ttgcagcggg gatcgccctc gccgtacttt ttcacggact tctcccacat ggcccacccc
    23341 agatcggccc ggacgaagtc gatggggagc tccatggcgt ccagcaccag catggcggct
    23401 tccatcatgt cgaagcccga atcgtccccc gccatgtagc agacctgata acggtctttc
    23461 ttttcgcgca ccttcatcac gttgcgggtc atgaacaaaa cctccctgga tcggttatgg
    23521 attctaaagt taggggcccg ggccgaaacc gaaggagccc ggagccccgg aggatagggc
    23581 ggcgcgtttc tcggggacct actttagccc caaggatttc ttcacgtggg gaatgagtcc
    23641 cccgtcttcg atgaggcccc ggacgaaatc ggggtaggga ggaaaggcga actccccctt
    23701 tttggtgacg atcttgcccc ggtcgaagtc caccgtcacg tcttcccccg cctcgatggc
    23761 gtccacggcc tcgggggaga cgatgatggg aagcccctcg ttgaggcagt tccggtagta
    23821 gattcgggca aagcccttgg cgatgatggc ccccaggccc cgctccttca ggcacgacac
    23881 ggcctgctcc cgggaggagc cgcatcccca gttcttgccc cccacgatga tgtctccggg
    23941 ggcggcattt tttgtgaatt cggggtcaag atcttccagg gccaccttgg ccatctccga
    24001 ccgttccttg atggtgtagg tgtactttcc ggggaagatg acgtcggtgt tcacgtcgtc
    24061 gccgtacttc cacaccttgc ccgtgatctt catggctacc gcacctcccg aggatcggcg
    24121 aagacgcccg cgatggcgga agccgccacc gtgacggggc tcgccagata gatgaagctg
    24181 tctttatttc ccatgcgccc cttgaagttg cggttcgccg tgctgatgca gacctcgccg
    24241 ggggccagga tgccttcgtg gtttcccatg caggggccgc agcccgggtt gcagagcacg
    24301 catcctgcgt ccaggaaggt gtccaggagc ccctccttca tggcctggcg gtagacgctc
    24361 caggaggcgg gaatcacgat ggtacgcacc gccactttat gtcccttgag caccgccgcc
    24421 gcttctcgaa ggtcttcgat gcgggcgttg gtgcagctcc ccaggaacgc ctggtggatg
    24481 ggttggccct tcacctcgtc gatgggggcg tagttgtcca ccgcatgggg cttggccacg
    24541 cagggcacca ggtctcccag gtcgtaggag agctcctggg cgtagaccgc atcgtcgtcg
    24601 gcccagaggg cctcccactc gtcgcttttg gccttgtcct tgatgaactc cagcaccttg
    24661 gcatccggag ggcacacggc gtttttcgcg cccatctcga tgcccatgtt gcacagggtc
    24721 atgcgctcgg cgaggctcat ggcctcgatg gcggggccgt ggaactccac ggacatgtag
    24781 tccgcgccgt cggctttgat gtcgccgatg atcttcagga tgaggtcctt ggcggagacg
    24841 ccctttttga actgccccgt gacggtgatc ttgaggctct cggggacctt gaaccagatt
    24901 ttgcccgtgg cccaggcggc ggccatctcg ctgcggccga tgcccgtgga gaaggccccc
    24961 agggcgccgt acatgcaggt gtggctatcg ctgccgatca tgatgatccc cggcagggcg
    25021 aagccctcct ggcacatgat ctggtggcag acccccccct ggctggacac gtcgtagaag
    25081 tgggtcatgc cctgttcttt ggcgaactcc cgggcttctt tgtggttcac cgcgtgctcg
    25141 tccgacgggg cggggatggc gtggtccatg atgaagacca ggcgctcggg tttccagacg
    25201 tttttcacgc cgattttctt aaaagtcttg gcgatggggg cggcgttgtc gtggctcatg
    25261 cagaagtgag gctccaccgt gaccacgtcg ttggcgcgca cctcgtatcc cgccgccttt
    25321 cccagagcct tctcggcaaa ggtcttgccc atggcgtcgt tccctcctta gtgaaaagct
    25381 cgcaccgctt cggtgtctcg tgctcgggta agatctggaa gcttactgcg ccacctgggc
    25441 cttgaggtag gggatgagcc cccccgccgt catgatctcg ttttccagat cggccacggc
    25501 gtctccgtgg aaagaggtct tctgggtgag gttggtaatg gtccccgtgg ccagatccac
    25561 ttccagctcg tctccgtcga cgatcttccc gtccttgatc ccctggctca gccccttcac
    25621 gaagagcacg gggtagccgt tatttatggc gttgcggtag tagatccggg caaaggattc
    25681 cgccaagacc accggcactc ccccttcttt gaggcagtac accgcgtgct cccggctgga
    25741 gccgcaaccg aagttccgtc ccgccaccac gaagtccccg ggcttgactt ccttggcgaa
    25801 cttttccttg ccggggtagt actcgaaggc aaactggggc attttcttgg ggtcggtttc
    25861 cgccagatac ttgttgtggt atatgaggtc cgtgtccacg ttgtcttcga agacccaggc
    25921 tcggccgcgc agttttttct ggtccatggt ttccttcctc cttggctttt ggctcgctta
    25981 gcggagcagg tcccggggat cggtgatgac gccccggacg gcggcggcca tggcagacat
    26041 ggggctcatg aggtaggtgt tgctcccctt gccgaccttg ccgatgaagt ttcggttggt
    26101 ggtggagacg cccacgtctc ccgagggaag gacgccgtag tgctcggcgg tgcacaggct
    26161 gcacgtgggg ttggtgaaga ccacaccggc ttccaggaac ttttggatca tccccgcctc
    26221 ggcgcagcgc gtcataacct cccgggtgct cggcgtgatg atggtgcgta ccgaggggct
    26281 cacggtgaaa ccccccgctt tcagaatgtc gaaggcggcg gcgatgtcct cgaaacggcc
    26341 gttggagcag gagccgatgt gtacctggtt gatgggagtt cccgccactt cccgcacggg
    26401 cttcacgttg tccggagcgt cggggcagga ggccaggggc tccagggagg acacgtccac
    26461 cgtcatgtgg gatacgtagg tggcgtcggc gtcgggggtg atcatcttcg tgcgctcgat
    26521 gacttcctct cccgcccggc tccgaagcca gtccaacacc tcgccgttcg ggaggatgag
    26581 gccgatcttg ccgtccatct cggtgaccat ggagcagagg gtgatgcgtt ccgcgaggtt
    26641 ggcgttctca atgacggggc cgtcgaactc cacggagcgg aaggacatgc cgtcggctcc
    26701 caggcgtccc accaggtagg tgagatagtc ccgcatgcag actcccttgc cgaaggtccc
    26761 cgtggcggtg atcttcatgc tctcggggac ccggaaccag ttggtgccct tgaaccacga
    26821 ggccacgatg tcggtgttgc ccacccccgt ggcaaaggcc cccacggcgc ccaggaggtt
    26881 catgtggctg tcggtgccca ggatcacgtc cccgggcttc accaggccat tttccagcag
    26941 cacgtgctgg ccgatgcccc agttgatgtc gaagaccttg gagatgccct ggcgcttggc
    27001 aaagtcccgg atggtcttct ggtttttcgc caccccttcg gagtgggccg gggcctggag
    27061 gtcaaaggta aaggcgattt tgtccggatc ccagaccaga cccttcttat cggtgacctt
    27121 gtcgaactgc tccgtgcagt tcgcgccgcc gaaatcccgg gcggtggaga gatccatgtg
    27181 gcaccacacc cgatctccca cctttacctg cttacccgat gcccgttcga tgatcttttc
    27241 gatcatggtc tttcccatgt ccgtacctcc tcgtgatcgt ctttcatatg aagttgcgcg
    27301 ctccgcctgg acgcggggga ggccccgagc ccctcggggc tacttcagga gccccaggaa
    27361 cgatttgatg gtttcgaagt tcacgaaatc acacttgttt atgaccaccg cctcggggca
    27421 gcggtagccg tcgtcccctt cgtgggcgtg gaccgcgatg cagtggcaga cccggctcgg
    27481 cacgccgttt cgcatggcga gccccgtgcc ggagaagggg tggcgcagat ccttgcccat
    27541 cttggatttc accactttgc cgtctttttt ctcgtactcc accagtttgc ccacgtcgtg
    27601 caacagcgcc ccggcgatga gaaggtcgtg gtcgagctgg aatccgccgt tctctttgta
    27661 gagggagttg aactcttcgt aaatcacctt ggccatgcgg gtcacgcccc gggtgtggat
    27721 caggtaagat gcggggcagt cggggatcag gagggtgaag gggatggtct ccatgtcctc
    27781 gggctcccaa ccccccgtct tcagcgcgtc ctcgtaggtg gccaccactt gcccccggag
    27841 agcctcgtcg gtgatccagg cgatctccgg caggagcttc tcgatgcgac ttcccatgcc
    27901 ttccgtgcct ccgcagcaac agcagttacc catgtgaaca tccctcctgt gtttttttgt
    27961 gacgacggtg cgacatttaa gaaacttccg gtgaggagtc tttccggagt cggggcccgg
    28021 gaagaactcc gatgggcctt ttttctggtg atcgaggctt ttttagggcc cttccagggc
    28081 cagggcctct tgcagggtgc ggtagatgcg ctcgatgtgg cggctggagg agcgggcggc
    28141 cccttcgccg tcgtggttca ggatggcctc gtagatctcc tggtgttcgt gcaagaaggc
    28201 gtccttgtcc gaaaggagcg agtatatgcg gtcgtgggtg gccatgatca aattgaaagt
    28261 catcttgacg atgttgacga agacgaagtt ctgggtcgcc tcggtgagca ccaggtggaa
    28321 atcgaaatcc gcctgggccc ggtccttggg gacggccatg ctcccttcgg tggcggagag
    28381 cacccgccgg aggcgggtga gatcccccga ccgggccttg cgggccgcct cggcggcgat
    28441 ggcgggatcc agtacccgcc gggcttccat gagctccaag atggcgccgt cttccagctt
    28501 tcgcgggtcg gaggctccgc cctcctcccc ccgcccccgg cggacgaagc gccccttgcc
    28561 ggggatgctc tcgatgagcc ccaagagctc cagcacccgg aaggcctccc ggagggagct
    28621 gcgactgact cccaggtcct ccatgagctg gcgctccggg gggacgggtt caccctggcg
    28681 caaagagccc gaggcgatga gggccttgat ctcgtccacc actttttcgt agatgcgcgt
    28741 ctgtcggacc cttttgtcta ccatatgaac cgctccttcg tggaccaccc agtgcacttt
    28801 ataccccaag gagacacttt gtgcaagagg gggcaagatg tcttgcaatt ctatggagaa
    28861 aggccgatcc aatgtgtcgg aaaatcccaa ttggtcggac caattgggcg gcccatgtct
    28921 cctcgtccgg cccgaggggg cgcctccggg gaaatcgagg gggcacgaca aaggccccgg
    28981 ggcgcccgaa ggggggcgtc ccggggccca tccggggggc ctttaggctc caaagggcct
    29041 aggagcggat catcaccaaa aacatcttga agggctccag ggccttcaag gcgtggggga
    29101 cgtcggcggg catcaccagg gcatctccgg gctccagggt gtggggcacg cctcccaggc
    29161 ggatttccac tcgcccggag aggacgtgca ccagggcgtc gtagggggcg gtgtgctcgc
    29221 tcagggcctg ccccttgtcg aaggaaaaaa gggtggcgtt tcccgtggga cgatcgacga
    29281 tggtgcggct caccacggcc ttctcctgga cggtcaccag ttcttccagg cgaaagggaa
    29341 ctcccttttc gtaggtggca tcggtcatgg ctctacctcc tttctaaggc atctcgctaa
    29401 aaaagtggag ccgggggacc ggctccacgg atgaggaatc gtcagcccag gatggctttc
    29461 aggtcggcct cggcggattc gccgatgggc tggatgttga acttgtccac cagcacctga
    29521 agggcttccg gcgatacgaa ggccggcagg ctgggcccca ggcggatgtt tttgattccc
    29581 agggagagca gggtgaggag gatgcacacg gccttctgct cgtaccagga caggatcaac
    29641 gagaggggca ggctgttcac gtcggtgttg aaggcctggg cgagggccac ggcgatctgg
    29701 atggcggagt aggcgtcatt gcactggccg cagtccagga accggggaag accctcgatg
    29761 tcgccgaagt ccagcttgtt aaagcgatac ttgccgcagg ccagggtgag gatgagggtg
    29821 tctttggggg ccttctgggc gaactccgtg tagtagttcc ggccgctctt ggcgccgtcg
    29881 catccgccga tgaggaagaa gtggcgaatt tttcccgatt tgaccagctc caccaccttg
    29941 ctcgccgccc ccagcacggc gtttcgggcg aagcccacca gaatggtctt gtcgtcgccg
    30001 tcttcgggga agcccggggc cgccagggcg gcctcgatga ccggggagaa gtccttgcgg
    30061 gtgacgtggg gtactccggg ccaagccacg agccccgtgg tgaagatgcg tcccttgtag
    30121 ctctcccggg gcttctggat gcagttggtg gtcatgagga tggccccggg gaaggcgtcg
    30181 aactccttct gctggtcctg ccaggcgccg ccgtagttgc ccgccaggtg ggggaacttg
    30241 tggagctcgg gatagccgtg ggcggggagc atctcgccgt gggtgtagat ctggatgccc
    30301 ttaccctcgg tctgtaccag gagctcgtgc aggtccagca ggtcgtgccc cgagaccagg
    30361 atggccttgc cttttttggg gaagatccgc acgggggtgg ggacggggtg gccgtaggtg
    30421 ctggtattgg ctgcgtccag gagctccatg acgtggaggt tcttctgccc caacttcaga
    30481 gccaagggga ggagggcatc caccgtgagg gggtcggcca tgatgccatc cagggcctca
    30541 tggaagaagg cgaaggcctc ttcgctctcc ttgcccagga tgtgggcgtg gtccgcatag
    30601 gccgccatgc ccttgaggcc gtagatcacc agccaccgga gtccccccac cgtgggaccc
    30661 cacttctcga tgtccgcttc gggggagaac ttctctccca ggcgcaccat ggaaggaatg
    30721 tcttttaccg actcctggca ggaggggcag cagagcttgt cgggggtctt tccggccttg
    30781 tgggccgcct cttcgtaaag cttatggata cgggacttca tgtcgcaggc ctttttgatg
    30841 aggccctcca gtcgagccgg gtcgaagttg acattggtta tcgtggtgaa aagggcttcg
    30901 aggacgaaga cgtccacgtc gtgatcccgg acgttgaact gccgggcccg gtgggctagc
    30961 tgaccgatct cccccgtgat gtgcaccaaa agatcctgta gcgccgccgt ctcggggtct
    31021 ttcccgcaca cgccgaagct ggtgcatccc gtacctttag ccgtctgttc gcactggtaa
    31081 caaaacatcc tttgcgcctc ctttttgtga tggtggaact ctattctaac acacgggaat
    31141 ccttccaggg gcctctgcgt tgatttttca tcctggctcg gccgtgcccg ggccccctgg
    31201 gccgccgcct tgcccctccg accgacgcca atactctccc agagtggtgt gagcgaggta
    31261 gtcttcgatc tgggccgaag ccgcctccaa aagtccgtcg aaacagcacc ctggcacggg
    31321 acacgacgga cgtcccaaaa ggcaccccgc cgggctcaag ggtccctcca ccgcctcgta
    31381 gatctccaga aaggagatcg tctccggggg cctggtcaat tccacccccc caccggggcc
    31441 tcggatggaa cggacgagac cctgatgggc caagcgttgg atcaccttgt agaaatgggc
    31501 ctccgaggcg gccacttcct tcgccagctc cctcaccgtc cagcggcgcc cttcgggaga
    31561 ggccagaagc agggccccgt gaagggccaa agatgcggct tcggacatgt gaatcaggcg
    31621 agccatggag atacctccaa tctggtggaa gaataccaat tattttcccc cttgtcaagt
    31681 tttttctggg acgaagggga gtgtcgtgct acactttcgc catgagaacc gatggaggcc
    31741 cgaccatgga aaaaagtgga ccgttttacg gttggcagct cgcggcggcg gatcgcctgg
    31801 cgggcaagag cggagtgttg gcggccccca cggggtctgg gaagacctgg gtggcctatc
    31861 tttgggcggg catcctggat cgggagggga ggatccacac ccccccggag cgggtgatct
    31921 tcaccgctcc catcaaggcc ctctccaacg agcgctatct ggacctgcgc tccctggggc
    31981 tcgacgtggg gctggagacg ggggacttca agaaaaacga aggggcttcg gtgctctgct
    32041 gtacccagga gatctatacc ctgaaatacg cggggcgtcc ggggattcgt ctcgtggtgg
    32101 acgagttcca ctacattttt tccgacagcg agcgggcccg gacgtacatc gacggcattc
    32161 gggccacggc gccggaggtg cccatcctgg tcatgtccgc cacctttggg gggccccgga
    32221 gcgtgcagcg ctatctttcc cggatcacga accgcccctt tgtgctccac gagagcgtgg
    32281 atcgggccac ggaactgatc tttctggaaa gatccgtacg ccgctttggg gagatccacg
    32341 acgccctgat ctttctcttt tcccgggacg gggtgacgga agtggcggac caggtggccc
    32401 ggagccgctc ggtgctcccc gacgaaaagc tccggaggct ggacgacatc gccgccattc
    32461 tgggggtgcc ccaggtgccc ctgcctctga tgaagggggt ggggacgtac cacgggagtc
    32521 tcctgcccaa ggaaaaactc ctggtggaaa cggcgttccg ggagcggctg ctggacgtgg
    32581 tggcggggac cgacgccctg gcgttggggg tgaatcttcc cgcagagacg gtgattttcg
    32641 cccagctggc ccactaccac agcgaggacc ccatcaccaa gaacgccttt ctccagatgg
    32701 cggggcgggc ggggcgcaag gggcacttcg atccggggta cgttacctgg ctggagcaca
    32761 gcccctggga aaatcggcgc ttcgacacgg gggccaccta tcgggagctg ctgcgccgcc
    32821 gtcccgaacc cgcccggatt ttcctgcacc ccgccttcgg acgtctgctg cggggggagg
    32881 tgacccccga ggaggaggcc tttgtggtgg cctcgggatc gctgccggag caggatttcg
    32941 tggtctctct ggacgacatc cgtcgggcgc tgcggaagat cggcacctgg acgaagcggc
    33001 tggtgcctcc ccatctgcgg cggcgttttc gggaggtgct ggcggacgtg tggttcgagg
    33061 agatggagct ggggcagaac ctggctctgg cggaattgtt cacggcggaa aaacgtccgg
    33121 acgccctgct ggcggcggag cttctggagc gctacgagag aaatcgcctc caggctctgc
    33181 tgaagatcaa gcgtttcgcc aacgcgttgc ccaagggata cggctttcag ggcatggacg
    33241 agcttgggcg agaggtggag cgcatcgacc ccacggtatt caccttcgag gagcggctac
    33301 aggaaatcca ggagagccgg atgggcgggc tctgaggcga aggggcttga gactgaaggg
    33361 gccttcgctg aggaggccta aaaaggggag gtaactcgga ggagggatgg gcatgctgga
    33421 attggtggat ctgacgaaaa agctggccaa gggagagtac aaggaattat cggggaggct
    33481 gagggaccga ctgggggagc tccagcgcca ggcccgggac cggggcattc cggtgctttt
    33541 ggtcttcgag gggtgggagg gggcgggcaa gggaaccctc atgaatgagc tcatcctccc
    33601 cctggatccc cggggtttcg aggtgcgcaa cctggggacc cccaccgagg aggagcggtt
    33661 ttaccccttc ctgtggcgct attggcgatg gacccccgcc cgggggcgcc tggtgctctt
    33721 cagccgaagc tggtaccgcc atgtcttcga cgatcggctg gaaggacggg tgacgggaaa
    33781 aaagctggaa gaggcctacg gggacatctt gaacttcgag cgtcagcttt ccacgggggg
    33841 ggcgctgttg gtgaagtttt ttcttcacat cagcgccaag gagcagaaaa agcgcctcaa
    33901 aaccctggag gataaccctg ctacggcttg gcgggtgagc ccccgggact gggagctcca
    33961 cgcccgctac gacgagcggc tccgggtcgt ggaggagatg ctccaccgca ccgatacggg
    34021 gcacgccccc tggaccgtgg tggaggccca cgaccgcgac tacgccgtgg taaaagtcct
    34081 ggccactgtg ggggaggccc tggaacgggc gctgaaaggg catgacgaga ccttccgaag
    34141 gggggatggg ttacggccag gaggggaggg agagggcccc cccccggagg agaagccctt
    34201 tccggcgggg gccctgcgcc ccacggtcct gggcaacgtg gacctctccc aggccatatc
    34261 gcccaaggtc tacgacgggg agctcaagga gctccaggag cgttttcggt cggctcagta
    34321 cgccctatac cgcaagcgtc ttcccctggt ggtggtcttc gaggggtggg acgcggcggg
    34381 caaggggggg tgcatcaagc ggctgaccca acgcctggac ccccggggat atcaggtggt
    34441 gcccatcgcc gctcccaacg atctggagcg gtcccatcac tatctgtggc gcttttggac
    34501 cgcctttccc aaaggggggc acgtggcgat cttcgaccgg agctggtatg gccgggtact
    34561 ggtggagcgg gtggagggct ttgcttcgga ggaggagtgg cgacgctcct acggggagat
    34621 aaacgaaatg gagctccact ggtgccgctt cggggcccgg gtactgaagt tctggctgca
    34681 catagaccag aaagagcagc tccagcgctt cgaggagcgc cgggacacgc cggacaaggc
    34741 ctggaagatc accgaagaag attggagaaa ccgagaaaag tggccccagt acgaagcggc
    34801 ggtggaggaa atgctctggc gcaccagcac gatccacgcc ccctggacca tcgtggaggc
    34861 caacgataag ctcttcgccc gcatcaaggt gctgcgcacc gtggtggaag gggcggaatc
    34921 cctgctttag ccccggatgc ccggatgggg gtgatcttgg gggcctttgg gtggagcccc
    34981 gggggacgat ggatccccct ctcggggggg gctcctccga ggccttcgtg gtatactgtc
    35041 tttcgggatt gagaccccga tattttttcg atgcgaggtg atttttatgc cgaatgaaaa
    35101 ggccttggcg acggtgcggg acctccggcc cgtggcctgg aaggcccccc ttcgcccccc
    35161 ggcgggggag gttttggcgc gtcactcctt gggccttcgc gacgtgagag aagccgaagc
    35221 ccggctggat cgcttcgccc ccctggtgcg gcgcctcttt cccgaaacaa aagatgggat
    35281 catcgaatcc cccctgagaa gcgtggaggc ctttgccgcc ctgttgggag aggagacggg
    35341 acgccccctt cagggacgtt tttttgccaa ggcggacaac atgctccgca tcgcgggatc
    35401 catcaaggcc cggggaggga tctacgaagt gctctccgtg gcggagcgca tcgccctgca
    35461 gacgggcttt ctggagatgg gggaaaatta cgcccggatg ggcgaccccc gggggcggga
    35521 gctctttggc cggtaccata tcgccgtggg atccacgggg aacttgggta tttccatcgg
    35581 caccatggcg gcggcgatgg gttttcgggc cacggtgcac atgtcccagg acgccaaaca
    35641 gtggaaaaaa gacctcctgc gggagcgggg ggccgccgtg gtggagtacg ccgaagacta
    35701 ctccaaggcg gtggaggcgg ggcgtttggc gtgtcatgag gtgccggggg ggcacttcgt
    35761 ggatgacgaa cgttccctgg acctttttct gggctacgct gtggcggccc tgcggctgaa
    35821 ggggcagctc gacgaggcgg gggtgacggt ggacggggcc caccccctgt tggtctacct
    35881 cccctgcggc gtgggagggg ctccgggggg gattaccttt ggcctcaagc tgctcttcgg
    35941 ggacgccgtg cggtgctttt tcgtcgagcc cacccatgcg ccctgcatga ccctggccct
    36001 gttgcttaaa aacccccacc tctcggtgaa ggacttcggc atcgacggca tcaccgaggc
    36061 cgacggcctg gcggtggggg cgccgtcggc cctggtctac gatctggtgg gggagctggt
    36121 ggatggggcc tacacggtgg aggacgaacc gctctaccgc ttggtggagc tgctttggag
    36181 ctccgaaggg ctcaaggcgg agccttccgc cgtgtcgtcc ctctggggtc ccctgatgat
    36241 gggggaagac cgtcctgggg tcacgcacct cgcctggctc acgggggggt tgttgatccc
    36301 ggaaaacatc ttcgccacca tgcgccaccg gggggagcac ggggatcggt tgcgggtgcc
    36361 ggaggggctc tagtccgggc cccgggatga gatcaccctt tatgtccggg gcgtcggggg
    36421 attcccggcg ccccgggttg ttttttgtcg cggggcgtcc tccgaaggta cgtccgaccg
    36481 gggccttgga cccgagcctt caggaggggg cgggagtcta ggagagccct agccccccca
    36541 tggcgggggg aagggggaag gtggccacgg cggggtcttc ttccggagag gcttcgcctt
    36601 cgaaggggcg ttccggggcc agggcggcgc tccagtcccg ccaccgcagg atttccaggc
    36661 gaccgtcggg acgttcggcc agggcggagc agctctccac ccagtcgccg cagttggcgt
    36721 agacgatgga ccgcatattt ttcagggcgg ggcgatggag gtggccgcag atgacccccg
    36781 cagacctctc ccgggccgtc tccctcagca cggcggcctc gaagtcgctg agaaacccca
    36841 cgacgtcttt tactttgtgc ttcaggtagt ccgaaaggga ccagggttcc catcccatcc
    36901 aggagcgcag ggctccgagc cccgcattga cgcccaaggc gacgccgtag gcccggtctc
    36961 ccaggcgggc gatccaccgg tggcgcttca ccacccggtc aaactcgtcg ccatggagga
    37021 cggaaaagcg ccggccgtcg gcggtgtggt gaagggcccg gcgacagatc tcgatgtggc
    37081 cgaaacggcg cccgtcgaag gcgtccagaa agtcgtcgtg attccccgtg atgtagcaga
    37141 tccgggtgtg ctgggagatc tgcaggagct tttggatcac ccgttgatgg gactggggcc
    37201 agcgggagcg ctgccgggcc ttccagccat ccacgatgtc tcccaccaga tataggctat
    37261 cgcaggaaaa aaaagacaag aaccgagcca gctcccgggc ctgacaccag gggttcccga
    37321 ggtgcagatc cgaaaggaat aaggcccgaa agggccgggg ccccgggggg gcgggggcct
    37381 cctggggggg aggcgatggg ggtgcgggca ttatcgggat gctcctttcc cgccgccgct
    37441 ccgaagggga tgggggatag gccctccggg gcgggatcgc acgagagatt ttcccaaaga
    37501 ggggcaccaa ggggacggcc ccatcgtaac acacgggtgg gacgacggag agaaaaaaga
    37561 agcgggggcg ccaaggcccc cgcttcccca gtgttctctg ctcttatttc ttttcgaagg
    37621 acatggaggc catgtgctgg tagcgccgcc agttgagctt gacgatttcc tgggcgcagt
    37681 ccacgagggc cttggattcc tcggggtgag agcgctggag gatgttgtag cggttttcgg
    37741 cgtagacgta atccgccaga ggcagggtgg gttccttgca gtcgatctgc agggggtttt
    37801 gcccctcttc gatgcgccga ggatcgtagc gcatgaggat ccagtgcccc gaggccacgg
    37861 ccttcttctg ctgctccagt ccctggctca tgtcgatgcc gtgggcgatg cagtgggagt
    37921 aggcgatgat gatggagggg ccgtcgtagg attccgcctc gcggaaggct cttaccgtct
    37981 gggcatcgtt ggcccccagg gacacccggg ccacgtagac gttgccgtag ttcatggcca
    38041 tcatggcgag gtctttcttg ccccggggct tgccgccagc ggcgaacttc gccacggctc
    38101 ccatgggggt ggacttggac atctgaccgc cggtgttgga gtagacctcc gtgtccagca
    38161 ccaggatgtt cacgttgcat cccgaagcca gcacgtggtc cagcccgccg tagccgatgt
    38221 cgtaggccca gccgtcgccg ccgatgatcc acacgctacg gcgcaccaga gcgtccgcca
    38281 cgccttccag gacccgggcg tcggggaggt caatgccctt gaggcactcc cgaagcttgg
    38341 tcacgcgatc gcgctgggcg gcgatgccct cgtccgtgct ctggtcggcc cccagaaggt
    38401 cttttaccag agaaccgcag agcttggtgt cgaggcgccc caggagctcc cgggcgaaac
    38461 cttcctgctg gtccaccgcc agacgcatcc ccaggccgaa ctcggcgttg tcctcgaaga
    38521 gggagttggc ccaagcgggg ccccgcccct cggcattttt ggcccagggg gtggtgggga
    38581 ggttgccgcc gtagatggag gagcatcccg tggcgttggc caccatggcc cggtctccga
    38641 agagggcgga gacgagcttc acataggggg tttctccgca tcccgcgcag gcgccggaga
    38701 attcgaaaag cggccggtag agggccaggt ccttcaccgt gccggggttg agcttgccct
    38761 tgtccgcctc ggggagcgag aggaagaact tccagttctc ccgctcggtc ttctgcaggg
    38821 gcagctgggg caccatgttg atagccttct tcccctcttc ggtcttgctc ttggcgggac
    38881 agttctggac gcacaggccg caaccggtgc agtcctccac ggagacctgg agggtgtatt
    38941 tcatcccatc gaactgcttc cacttggctt cggtgctctt gaaggtctcc ggagctccgg
    39001 tcagaagggc gggatcatag accttgcctc ggatgacggc gtgggggcag accagggagc
    39061 acttgccgca ctggatgcac agctcgggat cccagctcgg gcactccatg gcgatgttgc
    39121 gcttttccca ctgggtggtg gcggaaggga aggtgccgtc gacggggaag gagctcaccg
    39181 gcagatcgtc cccttcgagg atgatcatgg ggccttcggt ctggcggacg aagtccgggg
    39241 cctcggcggg caccgggagc agcaggtcga aggtgctggt gacctgcccg gggtttttca
    39301 cctcgaagag gttcgccagg gcggcgtcca cggaggcgaa gttctgctgc accaccgcct
    39361 cgcccttctt gccgtaggtc ttttggatgg acgacttgat ggattcgatg gcctcttccc
    39421 ggggaagcac gccgctgatg gcgaagaagc aggtctgcat gatgctgttg atgcggcccc
    39481 ccataccggt ctcatgggcc actttgtagg catcgatgac gtagaagcgg atcttcttgt
    39541 cgagaatgcg cttctgggtg atccggggca gcttgtccca gacctcctcg gggccgtaga
    39601 cgctgttgag gaggaaaacg gactccgggg cggcgtggcg gagcatgtcc agccgctcga
    39661 tgaaggagaa ctgatggcac gccacgaagt tcgcccggtt gatgaggtag ggggcccgga
    39721 tcaccttggg gccgaagcgc aggtgagaca cggtgatgcc tccggacttc ttggagtcgt
    39781 atacgaagta cccctgggcg tagttttccg tgtcgtcgcc gatgatcttg atggagttct
    39841 tgttggcgcc cacagtgcca tcggccccca ggccgaagaa gaggcaccgg gtggtcttgg
    39901 gatcctcggt ggagaagctc tcgtccacgg cgaggctcag gtgggttaca tcgtccacga
    39961 tgcccacggt gaagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    40021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngtggcg
    40081 gggagggccc ggaggaagtg ctccgccgag aaagggcgat agaggcggac cttgataacg
    40141 cccacctttt ctccctgggc attgaggtag tccacggttt cgtggacggt ctcggcgccc
    40201 gatcccatga cgatgatgac gcgctcggcc tcgggatcgc cctcgtagtc aaagagatgg
    40261 taggatcgcc ccacgacctt ggcgaacttg tccatggcgc gctggacgac ctccgggcag
    40321 gcggcgtaga aggggttgca ggcctcccgg gactggaaga agacgtcggg gttctgggcg
    40381 gtgccgcgga tcacggggtg ctcggggttg agggcccggg ctcggtgggc tgccacgagc
    40441 tcgtcgctca ccagggcccg aatgtcgtcg tcggtgagct gctcgatctt ggccacctcg
    40501 tgggaggtgc ggaagccgtc gaagaagtgg atgaagggca cccgggcctc cagcgtcgcc
    40561 gcgtgggcga tgagggccag gtccatgacc tcttgcacgg agcaggaggc gaggagcccc
    40621 acgccggtgc tacggcaggc catgacgtcg ctgtggtctc cgaagatgga aagggcgtgg
    40681 gaagccaccg tgcgggcggt gacgtggaga acggcggggg tgagctctcc ggcgatcttg
    40741 aagaggttgg gaatcatgag aaggaggccc tgggaggcgg tgaacgtggt ggtgagagct
    40801 cccgcctgga gggcgccgtg caccgctcca gcggcccccc cctcgctttg catctccgtc
    40861 acctggggca cggtgcccca gatgttcttc ctcccctggg aggaccattc gtcggaccat
    40921 tcccccatgg gggatgacgg cgtgatggga tagatggcga tgacctcgct gaccttgtgg
    40981 gccacgtggg ccgccgcttc gttcccgtct acgactacct tcggccgtga catggacatg
    41041 acgcctcctt cttgctcctt gaagttcctc cgcctattgg aggaggtatt aaatccagac
    41101 tcttccccgg ggggatcctc tggagtatga cacacaaaaa cacagcatgt atcatacacc
    41161 aaaagggatg gttttacaat tctcgaggag gaaaaactcc ggggctccgg ggggtgtgct
    41221 gtcatgggta gacctccgga gcgtcggtgc aggtgtaaaa ttacgcgcgg agggatcgcc
    41281 tagggtggat cgccccatgc ctccgccgag gggaaaagat cgtaggaaaa gaatctcgtt
    41341 ccagttcttg acctggagtt ttctaagtgg aagaataggg caggatggaa gcggagaagc
    41401 aaaagatcag aagaggtcga tcttgccaga gaggcctcgg gcgcgccgcc gccccggggc
    41461 ggtggcggca ggagaagtcc gggacgcggg tcggttttcg atgcgatgga tccccggggt
    41521 gctccccggt gacagcgagc ggaaaaagac gggatctttc cggagaaatt taggaggaat
    41581 ggaagcaaag aggtctcaag aaagaggagg ggtcaggacg tggtggaggg acagaaaacc
    41641 gtgttgattt gcgacgactc catgctggtg cgcaagaagc ttcgagatag cctggaaaag
    41701 gggtgtggcg tgcgcgtcat cgaggcggcg gacggcgctg cggcggtggc gatctaccaa
    41761 gaggaacgcc ctagcctcgt tttcatggac atcgtcatgc ccctgaaaga cggcatcgcg
    41821 gcactgcagg agatccgcgc cttcgaccct caggcccagg tggtgatggc gtcctccgca
    41881 ggaacccagg atcacctccg gagggccatc gatgccgggg cctcggactt cgtccaaaag
    41941 ccctttgcgg aagaccggct acgggagatc gtgcaaaagc tctgcaccac cgtgcaggga
    42001 ggcgcccctt gcggatgccc ggccgacacc tctttttcgg agaaaaaaga tgtttagcgc
    42061 gcttttgggg gaacacctgc tacgggagcg catcctgcag cctcgtcagc tgggcgaagt
    42121 gatggatcgc atggatcgga cccgcctcaa ggtaggggtc ctggccatca acgcggggta
    42181 catgacggcg gcccaggtgg atgaggtcca ccgggcccag accgccacgg accgccgttt
    42241 cggggacatc gccgtggaga agggctatct ttcccccctc cagatggatg atctcctggc
    42301 gtcccagcgc tacgaacacc tggtgatggg acaggcgttg atggacatgg ggctcatgac
    42361 catggcccgg ttcgaagagg tgcttcagga ttacaagaaa cggtacgatt tcgacgacgc
    42421 ctccttcgac gcggcgaaaa agggcgatgt ggactccctg atggaccgtc ttgtccgcgt
    42481 gcccgaaggg gaggagcgga ggatctttgt ggagtacctg gccctcttcg cccggcacct
    42541 ggtgcgtttc gtggaccgcc acgcggcgct ggctccggag gctccttccc tggattatcc
    42601 cctgccctgg gcctcgtacc aggtgatcca cggccccgtg acgcttttca ccgccctgtc
    42661 gggcaccgag gatctgctcc tgcgcctggg ggaggccttt gcccaggagc ccatccccgc
    42721 cttcgacgac ctggcccggg cctccgtgga ggaatgcctg aatcaggtca atggactgtt
    42781 tacggtaaac atgtccaatc gggggctgga tctggagatg gatcccccgg ggacgtcggc
    42841 gggggcccgc tacgcctccc gggacggagg
//
LOCUS       DAOOER010000012        41265 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_1039_length_41265_cov_9.164281, whole genome shotgun sequence.
ACCESSION   DAOOER010000012 DAOOER010000000
VERSION     DAOOER010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 41265)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41265)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41265
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1039_length_41265_cov_9.164281"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   57..2271
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=57..88
                     /rpt_unit_seq="gtcgccccccgcgcgggggcgtggattgaaac"
     assembly_gap    2274..2373
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2659..3651
                     /locus_tag="PL162_02955"
     CDS             2659..3651
                     /locus_tag="PL162_02955"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQF91025.1"
                     /translation="MSPWIFFRNALALGALMTLLAPLRGEGGVSVSVMLQWVPQAQFA
                     GFYVAQDRGFYARRGLDVRLVHGGPHRDSARALETGEVDFSTLFLATALVQRDRGVPL
                     VNVAQLVQRSSLVLVGREDREVRSPRDLAGRRVSLWGDAFEAPYRAFFDILAISVDAV
                     PQHRSVNLFLRGGVDACCAMYYNEYHAIWQAGLDEDQIEPFFLRDWGLDFPEDGLYTL
                     ASTATRSPDLARNIAEGTLEGWRFAQASPDVALDLVMRRAEDAGIPANRPHMAWMLTH
                     ILPAIFPDDGGSDSPGKLSREAYLRTARILERHGIIRHIDPFESFTLDGVSRVP"
     gene            3641..5584
                     /locus_tag="PL162_02960"
     CDS             3641..5584
                     /locus_tag="PL162_02960"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HQF91026.1"
                     /translation="MFPRVRTLAGRMILLLVLGGGGVLVALSGYNYYSSLKIFREELR
                     LRAQATVKGAANRVESVVRSVEKVTEELALALRILSPDAALRHALLAATLRDQPEIFS
                     ITAALEGSDGAVALLQAGRRAPGTFVRPRDPRSYPYATLDWFTLPRDLGRPAWSEPFS
                     SAEGGAPDDSPEERRQLRASYAVPLFLGPGPSEGFFGVVACDVSLAWLSAYLASLPLD
                     GGYAFLLSGNGTFITHPNQTYIMQETIFSVAEAFDDAPLRQLGQRMVRGETGFAALHN
                     PSTTKDYWIGFSPVPATGWSLGVLYPQDSLLEALGKWSRQELTLGALGLLLLAALVVM
                     VCRSVTSPLRELDTAVQRLSADNLHAPLPSVEGVDEVARLGRSFAIMVRDLRAHMAEL
                     QAATAARERIESDLRIAHEIQMSLVPRIFPPVRGLEIAALLDPAREVGGDFYDAFPLD
                     QDLWAFAVGDVSGKGVPAALFMAVTRTLLRGLAPTERAPGLLMARLNDELAAENDTCM
                     FVTLFYGVVRPSTGELRYASAGHPLPLRLAPGEPVRPLPRVSGPLAGPLGGMTFQEGS
                     ATLASGEALLGYTDGVTEALDPEGTLYGEERLVAFLEAHRALEPKELMEALRRELRCF
                     AQEAPQSDDITLMILRKSATADS"
     gene            6304..6906
                     /locus_tag="PL162_02965"
     CDS             6304..6906
                     /locus_tag="PL162_02965"
                     /inference="COORDINATES: protein motif:HMM:NF039801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6506 family protein"
                     /protein_id="HQF91027.1"
                     /translation="MFVRISRCGFLFLHPGEPGDLRWRKTFATPEFTMEIRGVASPEE
                     GETAARELRDGGVQILDLCGGFDDELTARIVAAVEGKIAVGARRPAAGSPPPPDAPRR
                     YGFIFKGPGMVPEHCQETFEAPGYFLEVRGVSSVEEGEIAAREMGDRGVTILGLCGGF
                     NDELTARIMAAVGGKVAVGTVRYAPEEAAKISHLLGLPEA"
     gene            7132..8730
                     /locus_tag="PL162_02970"
                     /pseudo
     CDS             7132..8730
                     /locus_tag="PL162_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
     gene            8782..9252
                     /locus_tag="PL162_02975"
     CDS             8782..9252
                     /locus_tag="PL162_02975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQF91028.1"
                     /translation="MVEKVVQSVAPTAQQKGLTLSCHIGDDVGELEGDQRRVEQILMN
                     LLSNAIKFTPQGRVSVTCSRENDTVTLAVRDTGIGIGAEDLATLFRPFQQIETGRTRQ
                     FDGTGLGLHICKRLGELMGGSITAESVPQVGSVFTLRLPAKGAEVHAPDEDPHH"
     gene            9224..9601
                     /locus_tag="PL162_02980"
     CDS             9224..9601
                     /locus_tag="PL162_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQF91029.1"
                     /translation="MPPTKILIIEDNEQNMYLMRFLLEKHRFDVREAWDGKEGIAMAL
                     REPPDMILLDIQLPLMDGYAVARELRSHEETSAVPIVAVTSYAMPDDRSRCLAAGATD
                     YIEKPIDPETFVRQISNHLPREA"
     gene            9634..11658
                     /locus_tag="PL162_02985"
     CDS             9634..11658
                     /locus_tag="PL162_02985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQF91030.1"
                     /translation="MNVLIVDDKAENLYLLQAILAGHEFFVASAREGREALEKAHQSP
                     PDLIISDILMPGMDGFALCREWRQAPELKDIPFMFYTATYTDPQDKQFALDLGADRFL
                     IKPMEPDAFMVEVRQVLEDRASFPGIAVRQPCATEAVIMRQYNQALIRKLEKKLDDRE
                     RDLALLAESERRLDEINRILRGVHLVSHLIAQEKEPANLLRLACEGLVRGQSFPSAWA
                     VRFPEGLSTPEGFQSGLPEALFRTFLKLFHDGELPPCCQKREDRTERPCHDRSSPCPL
                     EKISPSQPWIVHPLEHEGQIFGFLGLTPSPIAASTPEALSLSREVAADLAFALHTMRE
                     ENRRRQAEALRRAIFDHAQDGIILVRGDSLRIVMANEAMARLLGIPVEELVLLSIEDI
                     HAPEDLPRMRHLFEAVATKEPHLFCDVPLKRRDGSVVLTDVNSCPLELEGHLHSLGIF
                     RDITRRKEAEDRITQLLRDKELLLHEVHHRIKNNMASISSMLRLQARQQKNTDITHEL
                     DDAACRVQSAAVLYDRIYRSQRVCDISLKSYLPSLLQEILQSFPHQESIAVETALDDT
                     KACTNFVFGLGIVVTELATNALKHAFCGRDRGRLRLAAFREEERFVLEFEDDGVGLPE
                     EFSLDAPAGFGLHLVQILVLHFQGTITAKGNGGTRFRMEYPLAEVVAGCS"
     gene            complement(11787..12107)
                     /locus_tag="PL162_02990"
     CDS             complement(11787..12107)
                     /locus_tag="PL162_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HQF91031.1"
                     /translation="MTERFENVTVLTKANVYFDGKVISHTVLFADGTRKTVGAVLPGE
                     YSFGTGDREHMEIVAGTAEVLLPGSSTWTKVSAGEFFDVPGNSRFSYRCDGISEYICS
                     YIKE"
     gene            12343..13533
                     /gene="sfsA"
                     /locus_tag="PL162_02995"
     CDS             12343..13533
                     /gene="sfsA"
                     /locus_tag="PL162_02995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HQF91032.1"
                     /translation="MAPRRPGPGPQGSARESFSLYNDVSPVRGRFISRRDRFTVRCAP
                     EGSEESWAHLPNPGRLWEHLIPGATLRLLPARRGGMPWRVVGVDAPEGTVMLDTIRSN
                     EAVRWLLEHRLVPGFEEARVVRSEAVHGDNRFDFELEEGGKPLFLEVKSCTLFSGGVA
                     MFPDAPTERGRRHLEGLAALASRGTPGGVLFLIQSAAVRGFLPDFHTDPAFAEAFAAC
                     APRLRVRALGVDWTPELSLRLPGRPVPFLRDTLLRENHDRGTLLEALSPKAPVPFDLG
                     ERGTMLEPGHYVYGEDLPQGLHQAARRRRRGFALRLRGAPDPGASTATPLPIRSSTPL
                     AEEAFQRLAPLGEVLLRQTDPGSFLVRLPIPPLAHRAVVDLLFFLRMGRLAPPEEGPS
                     GGFR"
     gene            13563..14399
                     /locus_tag="PL162_03000"
     CDS             13563..14399
                     /locus_tag="PL162_03000"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQF91033.1"
                     /translation="MDKVILVTGCSSGIGRHLVTALAQAGYRVAASARRPESLADLPA
                     AMTLPLDVTALEGASEAVDAVRRRLGRLDGVVNNAGLACRGVLEELSEEALRHIFEVN
                     VFGPLRLCRAALPFFREQGSGIIVNVGSIASRMVIPVNGAYSATKAALEAFTDALRME
                     VAPFGVRVVLIKPGNIRTSFAEAAAERSRKTLENPRSPYREIYRRYLAEATKARRHDP
                     GPEVVAEAVLKALESPSPRAHYTVAAPWRFRLLMALGGNGRNTILGHVFRGRDAPPRK
                     PS"
     gene            14548..16032
                     /gene="mptA"
                     /locus_tag="PL162_03005"
     CDS             14548..16032
                     /gene="mptA"
                     /locus_tag="PL162_03005"
                     /EC_number="3.5.4.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase MptA"
                     /protein_id="HQF91034.1"
                     /translation="MALSNGKAFPPQARGHRVFLALGTNLGDRQAHLIQALQYIKSRL
                     SLEAVSPFYETDPVGYTAQPLFLNAVCEGYTSLSPQNLLAFVKWVERHMGRTPTFRHG
                     PRVIDVDVLFYDDLVMSSPGLEIPHPRLAERAFVLGPLEDIAPDFVHPGLKKTVRELA
                     LQVGRSGIRPAPRALPFALEGDVQQGHPEVPLGLSRVGISNLRRIVRLGTEDRVALFY
                     ATMDLFAHLRGDRAGVHMSRFSEILEEMGEEISLPPTPDLESLAERIALQIARRQEAA
                     SSEVHIRAQFLVRQETPLSSRRSDELYTFLGYAASDGVTVRRAAGVEVDGLTACPCAQ
                     DMVRGAALARLREAGYAPEEAERIVSLVPLATHNQRGRGTLILGCDPKIPAEHLVAVV
                     EAAMSSETYELLKRPDEFFVVEKAHRRPRFVEDVVREMLRHVVEAFPDLPDDTFVLAR
                     QENLESIHQHNAFAEASALLGSLRRELAGGPPASGPSLEGWLRG"
     gene            complement(16043..>16253)
                     /locus_tag="PL162_03010"
     CDS             complement(16043..>16253)
                     /locus_tag="PL162_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91035.1"
                     /translation="YPYPYPVPPPIYRWGPPRPYPRPRPIVPPPPIYRRRPRGPREYE
                     LLMQLMMMGIMIDQNRYRDGGYDGY"
     assembly_gap    16254..16353
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16354..16580)
                     /locus_tag="PL162_03015"
     CDS             complement(<16354..16580)
                     /locus_tag="PL162_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91036.1"
                     /translation="MKKYAVVFVVLGLLLGASGAWAEGEGGSKGSTAKGGAQWFSPGP
                     GYRGFPPYEPRPHPVMPPPPRYRDFPPGIYP"
     gene            complement(16978..18096)
                     /locus_tag="PL162_03020"
     CDS             complement(16978..18096)
                     /locus_tag="PL162_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQF91037.1"
                     /translation="MKIADFGVEIWMNEYETRAKFNIAETCVESMTVHELLALCGDEE
                     RHLEVLRRIHLTYGDIPGSERLRGAVASLYKGRSAEEVLITNGGIGSNFIALWSFVEP
                     GDEVVALLPTYQQLYALPESFGARVTRLRARRENAFRPDVEELRRAVTHRTRLIVLNY
                     PNNPTGASLDEDAMREIVAVADRVGAWILCDEIYRGLEHQGAYGAPSFTDLYPRAIGT
                     GSLSKVYSLAGLRTGWIVAPREFLEECSRHRDYTTISCGRVNDYLAALALEHRDRVLE
                     RNLALVRSNAAVLGEWVRQEPLVSWTAPRAGTTALLRFQRDQGSEAFCRELFDATGTM
                     LVPGSCFEFSDCSARIGFAPHTETLRGGLGALSAFLHA"
     gene            complement(18093..18833)
                     /locus_tag="PL162_03025"
     CDS             complement(18093..18833)
                     /locus_tag="PL162_03025"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQF91038.1"
                     /translation="MTALGSLGRDRPPQRRGDTAVEGARRGILELVLKGTFKGGERLP
                     TEALASRLGVSRTPVREALRHLAADGVVEMVPRGGARLVCPTPAEVLETAEIRGHLEA
                     LAVRKAVARLDPLGEARLEACLEEEGPSRGLGTLGELFPERLGLHRVIAELAASPVLA
                     ETLEVFFRRTAVYALLLPLPEEELIASAREHREIVATMIRRDADGAEALIRAHASLQG
                     KVDAAMLRERKWQKGASSRTARGEGDRP"
     assembly_gap    19004..19103
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19104..19655)
                     /locus_tag="PL162_03030"
     CDS             complement(<19104..19655)
                     /locus_tag="PL162_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HQF91039.1"
                     /translation="MRPEMEERGWPDITEIVRAHKFLRHRVNETPLEPSPDLGEITGA
                     EVFLKWENQQKCHSFKIRGALYRMFSLTEEERWRGVGTASSGNHAQGVAVAARALEVH
                     ARIFVPEGCPEVKRRAICQLGGGFVTLEVFGALYDDTEARALEICRREGRIYVSPYED
                     PWVVAGQGTVGMEMFLQEPDLDTI"
     gene            complement(19755..20774)
                     /locus_tag="PL162_03035"
     CDS             complement(19755..20774)
                     /locus_tag="PL162_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HQF91040.1"
                     /translation="MKSYGMRYLNARDLRALLSMEDAMACVERAYRWHVSGQGVRWPV
                     ISQVFEPELRDMDIKSGWVREGEEGFPRGFFGLKVMGYAGDNPVERGLSALAGLVVVM
                     DVETGHPLGLVDGASLTELRTGAAGAVGARLLARPGIRRGLILGTGSQARAQLAGIAA
                     ALPHVRDVAVAGRNALAVGRFVGDMAPFFPQISLRPLGWNEVPAAAREAELIVTCTPA
                     STPILWKGWISPGTHVNAIGADMPEKQEVEASLLEEARVFADDRAQAFSRGECHHARD
                     RGFLEEGSVREIGEVLLGRAPGRSSEEEITLFDVTGMALQDLAGASRALARAAELGAG
                     QLLVE"
     gene            21224..21781
                     /locus_tag="PL162_03040"
     CDS             21224..21781
                     /locus_tag="PL162_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQF91041.1"
                     /translation="MKNAVIDVIRSRRSVRRYRPDPVPPEALEAVLEAALWAPSACDG
                     QPWHFTALVSPERIAQIDREACAFLARAPLDWAAAMGRAANFRIFYGAPAVVVVSGLK
                     DAEFHTAVDCAIAAQNLVLAAESLGLSSCWIGLTHWYFQSEGARRDLEIPEGYEPHFA
                     IALGYREGPAPEEPERRGHPVTVLR"
     gene            21929..22609
                     /locus_tag="PL162_03045"
     CDS             21929..22609
                     /locus_tag="PL162_03045"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HQF91042.1"
                     /translation="MDRSTWWQGVKSMVPLAVSIAGCGVAWGTLARQGGMTLPEIFLM
                     SSVVFAGASQFVAVGMLGAGAGMGEIILTTLILNLRHLLMGAALAPYFRRLAPWRTAL
                     LAHGLVDETFALAAERFRTTGGDAAFFLGTGTLAFVSWNAATLLGAFVGSGTLNPRDY
                     ALDFAFLGAFTGLLVPQIRSRSTLLAFLAAAATALGGRFVASERTVVLLSAVAAAVAG
                     VWGERHAN"
     gene            22599..22919
                     /locus_tag="PL162_03050"
     CDS             22599..22919
                     /locus_tag="PL162_03050"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HQF91043.1"
                     /translation="MRTDMVLLILGMGAVTYATRFTCLFLVGRYALPPLVTRALAYIP
                     IGILTAFVVPALFFSAPGDGAGGTSEMLWGGLASAAVAARWGHVLGAMLAGMAVVVLL
                     RWSF"
     gene            complement(22964..24358)
                     /locus_tag="PL162_03055"
     CDS             complement(22964..24358)
                     /locus_tag="PL162_03055"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HQF91044.1"
                     /translation="MKKFLERIDGTRLLLHPGAVPGMRVPGMIFADEYIASLREDDNA
                     ILQVAHVATLPGIVGYSFAMPDIHWGYGFPIGGVAAFDAEEGIISPGGVGYDISCGVR
                     LLASSIAAKDLGRVVDKLLPALFSAVPSGAVSGGGKPLSPRDLDQVLEGGVAWAVKHG
                     FGTEGDGEHTEEGGCLKGALPGEVSERAKDRGRTQLGTLGSGNHFVEIQMVDEIYSEK
                     DAARLGLIPGCVAVMIHCGSRGLGHQVCDDALKIMRRAMGHYGIDVPDRQLCGVPVSS
                     EEGQRYMGAMRAAANFAMTNRHVIADAVRRVFKAFFPREPLYTVWDVSHNLAHLETHR
                     WDGRKRTLCVHRKGATRAFAGLPVLIPGSMGTASYVLVGTPQAEESSFASTCHGAGRT
                     MSRTEALKRTGAKDLLRSLAKRGVSVMAENLHTLGEEAPEAYKDVSHVVDVVQGAGLS
                     LKVARLRPVAVIKG"
     gene            complement(24375..24791)
                     /locus_tag="PL162_03060"
     CDS             complement(24375..24791)
                     /locus_tag="PL162_03060"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HQF91045.1"
                     /translation="MGWKIIDHTADAGLEVWATSWHDLFLQAAEGFYCLCLDGAPPLP
                     PEGTAGKSRVLRLETLDLEELVVSWLHELLFLLESEGVFFHPTSLTVTPHPPALVAEG
                     LLFPSPGGRIAVKAATYGGMVLESSPHPFLRLYLDL"
     gene            complement(25161..28349)
                     /locus_tag="PL162_03065"
     CDS             complement(25161..28349)
                     /locus_tag="PL162_03065"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HQF91046.1"
                     /translation="MPSTEHLAYLRRADFSFTRQLGSVWFDDPRDVPELHHRVRADIL
                     ARVEASAQAGETPLGIVLKGDAGLGKTHLLGELRRRATERGVTFVYVDMTDVRSFDHT
                     LLLGLADSLTREVEGVSQLRHVIAALFVEAEDISGRSATLAQGLEYAAEVATLGENDL
                     RGATKVMVDALWQRHRTRFTHPSLDVIRALMGMNNEACLRIWSLASAWLQGSKVDDFD
                     GMQLGLVQCEPDPGHLIQGIFRMLSFRGPTLMAFDQLDNCFPLLDAARHAPDREEGKT
                     AKLVLGDIGKGFGALRDRLNSSTLTVVACFGDTWDHLKKLVPASFADRYTEPHRLLPV
                     SESSIARDLVERRLDAARGSFSPPYSTWPFAPEFFSAVETYSPREILRACHRHRDACL
                     AHGEVRELGSPETDPFREALPAVSSRFQALDGRLEELRASVDIAPYLEDDDRAEGALG
                     ELFAAGCRRCVEELETPENTFFEVESASGGRRSCPAIHLRWKRMVTGAGGVDAEWHVC
                     LRVLLRENHTAYNHRLQAAMVESGIARHLSRRRLVILRPEGIPLGGKVGPRITEDFLK
                     AGGVFMPPVEDDLRTLAAVVRMGEELSGASAPEEEGAFKVWLRERKPLSSLESLAPLR
                     LMIGGGDGGSGEGVSQLPEARNHSSDDAPVVVLGDPGETTRARLSADDLARHVVVLAG
                     SGSGKTVLLKRLVEEAALAGIPSVVFDSAGDLAQLGDRWPDPPKGWDPGDDLRAERYF
                     ETAEVRIWTPGCDRGRPFALRGLPDFPALRDDADAFQLATTVTVDMLTDLAVVGSGAK
                     AKRMAGVLAAAVKTFAQGDGGDIHAFLGFLRQLPPGTAGEISAASALAREAADNLQSA
                     MFTAPVLFGEGPLADPEILWGRPLEGGRTPVSVVSLVGVPGDRNRQIVVGHVVLSLLT
                     WARRHPPIQAPGRMNGLIVLDEAKDFLPSVRTSACKQGLQRLAAQGRKYGLGLVVATQ
                     NPKDLDGGSLSNMSTWFCGRANTPRIIDQIRSFLEDRGSVAAEIATLESGAFQFASPR
                     GAPRRVRVPLCLSHHPDRAPLTEEEICERARR"
     gene            complement(28379..29017)
                     /locus_tag="PL162_03070"
     CDS             complement(28379..29017)
                     /locus_tag="PL162_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91047.1"
                     /translation="MEVQGKALEWFYCLLLREARGAEDISATELHRQVSLKKTADLKP
                     LTEGGLVEITRRGRSKYAAVTDEGWRWAHAHLGAMAERVATKRGLTRGDREMLAFLFR
                     VVEGWVQKGGALASIFAPAPPEDGDPIRRIIDVMGRLAKSQGGDRLPLALVKGALAPW
                     SPDRVDALLLGLLKEGRVSLEANNDVSTLTDEDRRAAIRIGASDKHVVVLMP"
     gene            29342..29773
                     /locus_tag="PL162_03075"
     CDS             29342..29773
                     /locus_tag="PL162_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91048.1"
                     /translation="MTPILIRHYVHPQRSEAENATGLTLGRLISAHAPRFAALDLSLD
                     LEVVPCDAPEDRNRVTFSYPVPSDDEEPPQDRERSLEDLLGLGVVVSPGAPHRTLVYE
                     GQAYDAIPPGLLADGLLRVAMALMGGGGCGSSCAGCQGCGA"
     gene            29891..30418
                     /locus_tag="PL162_03080"
     CDS             29891..30418
                     /locus_tag="PL162_03080"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HQF91049.1"
                     /translation="MDGVWLVTRGGDKGETSLGDGERVPKDSPRVETYGTLDECQAAL
                     GLARSLCPFDDLGQEILRLEDHLGSVMGYFAAYPGMEEPDVTLLDGVVERVRGVVGDS
                     FSFVRPGDSAPGAALHLARTVARRAERSAVKLYRMNQLSDQAYAYINRLSDAIYALSL
                     WADKASKDRTAPSSK"
     gene            30581..31255
                     /locus_tag="PL162_03085"
     CDS             30581..31255
                     /locus_tag="PL162_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQF91050.1"
                     /translation="MPDNPLSPTQNIDLRQIVYEKIKEAILDGIIHPGERLSEVDLAE
                     KLTVSRTPVREAIRQLAETKLVALIPRKGAYVVLLSVQDAKDLYELRSALECCALTHL
                     CQNPPAEALQEVRAVFDGFNDSMSQFIHYDRQFHSIIRSGSGNKFLNSVLDNVVDLIQ
                     LCRRYALEVVPLATSAAEHVGIIDAILARDVEKSQRLLRDHLRRASESLATYLEKHPE
                     FSRQTP"
     gene            31295..32080
                     /locus_tag="PL162_03090"
     CDS             31295..32080
                     /locus_tag="PL162_03090"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HQF91051.1"
                     /translation="MEQGWGFVIYKAVASLALPPGLLVLGLALLGLGALRPPRRRGLG
                     FTLLTLSVAIYGMSTPFGAQALLGTLESPWKADIPPQDRRWGVLVLAGGVIVRDGEPA
                     ELNSFTLARLVGGWEAARQGPWPMIVSGGPSLEAPQAPSLGALMEERLRRWGYEGTVW
                     REETSRNTWENMTASKAIVVSEDLEGVIVVTDAFHMSRSMAMARRALPVAVRPYPVGF
                     LVDRRPITFWDFLPNPGSLHHSMIGLKEYLGLFAYDLYGFLFS"
     gene            complement(32136..33809)
                     /locus_tag="PL162_03095"
     CDS             complement(32136..33809)
                     /locus_tag="PL162_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQF91052.1"
                     /translation="MEERLAPSLLRRLRHAPDEGTFARELLEFLCDRFLAPLAVWRCA
                     PPRRAAWLFTSMGRRLNPLSPEELASLDAGRPEGPGALGRKNGVILGGVEVASSMDAV
                     VSGISGDEWFGVGNPAGGLSAGKIRDLEGVAVAAVMALDAFYGRMMADKIRLGGHLLR
                     TPLWTLELALDGGTCPPSEDGRNRVTAALALLQGGIDVMALPDMAHLGSVFLACHDVS
                     RRIQSLGEAWAFLEKREWAFSAGRFPPRGRLSQGAVLLLALMIQYAYGERGAQGVACI
                     LRPSGAEMECVLEAGSFTGPPGVLSREVRYLLDMFSLSWQESASAGGLLWKIRVPLVL
                     GLRRKDVGKRLTPPRVLVVDDNATNRAIIADALKKAGCTVDTAEDGLDALRIVGLWSP
                     DLVIMDILMPHMDGIAATRELRRRGFTVPVVALTAGGAGTALEALEAGMDDYALKPVP
                     RKLLWDLVAQWTGYDGAVSPPQDEADRLSMEEAFAAELPLIRTALRRAWVAGNEEEAR
                     ILAERLGSSAAQCGRDDLKRLAEEMEEALGEGRSAEPLMARLFEGEERF"
     gene            complement(33948..35177)
                     /locus_tag="PL162_03100"
     CDS             complement(33948..35177)
                     /locus_tag="PL162_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HQF91053.1"
                     /translation="MRPRTLAEYVGQRHLVGPGAPLRRLVDQGILPSCILYGPPGVGK
                     TTLVRLLAEGTRRGLFEINAVSARVGDLRELVERARRRKQADGGRGAVAFVDEIYHFN
                     KIQQNALLPAVEGGDLILVGTTTENPYFEINKTLLSRMVVFDLKPLDEADLREILRRA
                     LDDRERGLGTLEVAVPDEVLGALARGAGGDARQALLRLEVAVSSVAVGGGKVLTPEAG
                     AALLPRGALRFDRAGDDHYAIISALIKSIRGSDPDAAIYWLARLVASGEDVRFVARRL
                     VISAAEDVGLADPQALVVATAAAEAADFTGWPEARIPLAEATLYLAAAPKSNSAYGAI
                     GAAVRAVEGGNLQPVPEHLRPSGSGYRYPHDDPRHWVPQAYMDRTERFYFPGVLGEER
                     GMGQRLARFWRRFADQN"
     gene            complement(35249..36238)
                     /locus_tag="PL162_03105"
                     /pseudo
     CDS             complement(35249..36238)
                     /locus_tag="PL162_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     gene            complement(36728..37417)
                     /locus_tag="PL162_03110"
     CDS             complement(36728..37417)
                     /locus_tag="PL162_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91054.1"
                     /translation="MGWKRWGAAAVAAWGIFWAGMAFGEEGVFPLTSGARWHYEGTAQ
                     WTEEGAEGPKVAPLSWDMEALEVFSSSGSTVAVMRGMVSETSGYTPGQEPGYAVLVER
                     ASHVELFPQASELEARAFAKSLAEDPSTGTEGDLLVALPLAKDKAWGGDEPRDDTMYQ
                     WYVEEERSDVPSIPGTSPSGKAFVVVYRTNPDTTTMELVPGVGITRWSYEHHGTASAV
                     DLTLGSFVGGK"
     gene            complement(37677..38906)
                     /locus_tag="PL162_03115"
     CDS             complement(37677..38906)
                     /locus_tag="PL162_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQF91055.1"
                     /translation="MSLRSTPLDLWCARCLGEGAPLAGRAALEAFQKERLRALVARAA
                     QDSPLYASRLRGREGAPLEELPFTYPSDLAGREGDFLTVSQSEVARIVTLATSGSTGP
                     PKRLCFTYADLAATREFFRVGLTAFTPPGSRVTVLFRGDRPGSVGALLREALGELGCA
                     VEVGGVGEDPRKTAARIDAFNPRVVVGMPEALLRVARVARHVPEAVLASGDMFPPALR
                     RRIARLWPGEIFDHYGLTEVGWGMALECGAHEGGHLREGEVLTEVVSPGGHPVPSGSW
                     GEVVITTLTRSTFPLIRYRTGDEGRLIPGPCPCGSPLRRLEVRGRLGTAEAPGLYDVA
                     EILWGVRDIRDFRVGREGPSRRVVLELEMEQGGVLPEEIRSALSLLKGEWSEAEIRPV
                     PGPRPEPRPLKRGWETF"
     gene            complement(38903..40225)
                     /locus_tag="PL162_03120"
     CDS             complement(38903..40225)
                     /locus_tag="PL162_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQF91056.1"
                     /translation="MRLPDETESVCPVCLRPVGALLVPEGDEVFMKKSCPEHGDFSCV
                     VWRGVESWKAWRGPKAPPAPELGRNPACPGACGLCSFHAQKTCAVVLDVTSRCLLRCP
                     VCFADAGSEDPDPPLELLLERMEKMRPQVGEAVLQLSGGEPTLRPDLPLLVEGARKRG
                     FGAVQLNTNGLALAEDPALAPRLRDAGLGWVFLQFDGLSERSSRRLRDKDLREPRERA
                     VEACARAELGVVLVPTLVRGVNDGELGDLVRFALGHFPTVRGVHVQPMSFFGRYSLEP
                     QDHRRITLPEVLALLEEQTGGMVRREHFHPAGCEHERCSCRGTYRVEGPGRLVPLGGT
                     PCACRSQSAEEGRSRAVESVRRRWGASLEKRDDDGAGDALDRFLRQEGRVLFSVSAMA
                     FQDAWNLDLRRLRECCIHAAAPDGRLVPFCAWNLTAADGTPLYRRGAG"
     gene            complement(40222..40686)
                     /locus_tag="PL162_03125"
     CDS             complement(40222..40686)
                     /locus_tag="PL162_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HQF91057.1"
                     /translation="MTLEGENDLREWIVAWAREGFCCAQIMVLAGLAYRGEEDRNLVA
                     AANGLCQGAFSRGNTCGALTGAACLLGLYAGKGEAEDLRDPRLRLMAEELNQWFRETQ
                     TGGTGGVRCGDLLGETVREPDPLRCLHLVQHTLTRTLEILQSHGVDLFGASS"
     gene            complement(40683..>41265)
                     /locus_tag="PL162_03130"
     CDS             complement(40683..>41265)
                     /locus_tag="PL162_03130"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQF91058.1"
                     /translation="LPVIGVCGGDAGRGGRLELLGRGGWQAVGVDRSPERLRCAARRG
                     PVVEGTLEALPFDDGSFDGVVCECVLSQSADPRSILGEFFRVLRPGGVLGVGDLFLPE
                     NVPPGGGPCAGCVGGARRGEEMERSLAGAGFLLRHREDHSRAVRELAARLVWAGILSC
                     EAWTAPFRRGYALWVSGKPSSPGPGSGQHAGRG"
BASE COUNT         6880 a        14479 c        12919 g         6687 t
ORIGIN      
        1 cctcctgggg aaaaaacttt gcaaatctgg ggatttgcct cttgcaaaat acaatggtcg
       61 ccccccgcgc gggggcgtgg attgaaacat cttgggttta cggccacgcc gaaccgcaac
      121 gagtcgcccc ccgcgcgggg gcgtggattg aaacgtctcc tgcgggagcg ttacgagcat
      181 gagcgtttgt cgccccccgc gcgggggcgt ggattgaaac gataacgcca aaaatcgccg
      241 agcggaccgg tcatcgtcgc cccccgcgcg ggggcgtgga ttgaaacacc cgagccatgc
      301 caactacctc ccccactctc ggtcgccccc cgcgcggggg cgtggattga aacagcgctt
      361 ccacgttcgc atcgtactgc cggaatgtcg ccccccgcgc gggggcgtgg attgaaacct
      421 gtcagcggac gcgcggcgct cctcaatatc tcgtcgcccc ccgcgcgggg gcgtggattg
      481 aaacccttcg tctcgacaag cggcttgaag agcgcctcgg tcgccccccg cgcgggggcg
      541 tggattgaaa ccagaggtgg cgcaccaccg ttttcaccgc catctcgtcg ccccccgcgc
      601 gggggcgtgg attgaaactg ctctgccagc atggcccgcc gcgtcctgat ggtcgccccc
      661 cgcgcggggg cgtggattga aacaagagct ggctgggatc ttccgggaca ttgatagtcg
      721 ccccccgcgc gggggcgtgg attgaaactg ttctcccggg cggtggaaac gtccaaaacc
      781 tcgtcgcccc ccgcgcgggg gcgtggattg aaactgtgac ggccaacggc cagtgcgatg
      841 tctttgatgt cgccccccgc gcgggggcgt ggattgaaac tcatggtcgc gctgatctgt
      901 accgcgggat ggcttgtcgc cccccgcgcg ggggcgtgga ttgaaacttc ggagaaggag
      961 tcttcgacgc gcgagacact acgtcgcccc ccgcgcgggg gcgtggattg aaacggcaac
     1021 ggcacgcgcc ttgtcggcga gagcttggtc gccccccgcg cgggggcgtg gattgaaact
     1081 ttttgctccg gatcgtagct gccggaaacg gccgtcgccc cccgcgcggg ggcgtggatt
     1141 gaaacgcaac atagaggaag gccatgtcac ggcgatcaag tcgccccccg cgcgggggcg
     1201 tggattgaaa ctttgcgccg acggggcatc ggaggatctg acgcgtcgcc ccccgcgcgg
     1261 gggcgtggat tgaaactctg tctctggaac gtttccgccc actctcgcgg gtcgcccccc
     1321 gcgcgggggc gtggattgaa acactgtccc gtccggcatc gccgtctcta ctaccagtcg
     1381 ccccccgcgc gggggcgtgg attgaaactc gtgaaggaaa tcctccgcaa cgacgaggaa
     1441 atagtcgccc cccgcgcggg ggcgtggatt gaaacacgcg gctcacgtcc cacgcgaaac
     1501 gctcatgctc gtcgcccccc gcgcgggggc gtggattgaa acgtagcgcc gcgggcaccg
     1561 cttgtaggcg cgcagcgtcg ccccccgcgc gggggcgtgg attgaaacgg agcgagctgg
     1621 acgagcggcg cccgggatgg gttgtcgccc cccgcgcggg ggcgtggatt gaaacgtcga
     1681 gggggttaga tgccggcacg ccgtttttgg tcgccccccg cgcgggggcg tggattgaaa
     1741 cgtcatggcg gaacaggtgt tggtgagagg gcaaagtcgc cccccgcgcg ggggcgtgga
     1801 ttgaaacctg cacgtgctgg cgtaccgcgt ccgcgatggc cgtcgccccc cgcgcggggg
     1861 cgtggattga aactcggcaa tggtccgggc gatggattga gctgcgggtc gccccccgcg
     1921 cgggggcgtg gattgaaacc ggagaggggg cggggcatcg gttgtcccgc tccagtcgcc
     1981 ccccgcgcgg gggcgtggat tgaaactggg ggtcgaaaag ggggcacgcg ccccccgtca
     2041 gtcgcccccc gcgcgggggc gtggattgaa acaaacaccc tccgatagtg ggccagctcc
     2101 tgatccgtcg ccccccgcgc gggggcgtgg attgaaacgc cgggccagct ccagcatccc
     2161 cttgtaccca atcgtcgccc cccgcgcggg ggcgtggatt gaaacgtcat ccgattcatt
     2221 gctccacccc atcgtcatcg tcgccccccg cgcgggggcg tggattgaaa cgtnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntccgccg attagcggag gttgacgtcg
     2401 ccccccgcgc gggggcgtgg attgaaacgt ctggatacct ccgccgatta gcggaggttg
     2461 acgtcgcccc ccgcgcgggg gcgtggattg aaaccagtcg ttgccgctcc ggttgtcgcc
     2521 gaagttttgt cgccccccgc gcggttcttt tgctatactg gtttccgaaa gttctttcgc
     2581 tggagcacac gctctgccct caccaaaagg agcccttggg cgaaagaccc cgtcgccaca
     2641 cggcgaggag gaaacgacat gtccccttgg attttctttc gaaacgccct cgccctgggg
     2701 gccctgatga cgctcctggc ccccttgcgg ggggagggag gagtgtccgt gtcggtgatg
     2761 ctccagtggg tcccccaggc ccagttcgcc gggttctacg tcgcccagga ccgggggttt
     2821 tacgcccggc gggggctcga cgtccggttg gtgcacggcg gcccccatcg ggattcagcc
     2881 cgggctttgg agacggggga ggtggatttt tccacccttt ttctcgccac cgccctggtg
     2941 cagcgggacc ggggggtgcc tctggtgaac gtggcccagt tggtccagcg ttcctcgctg
     3001 gtgctcgtgg gacgggagga ccgggaggtg cgctcccccc gggatctggc gggacgccgg
     3061 gtgtccctct ggggggacgc cttcgaggcc ccctaccggg catttttcga catactggcc
     3121 atctccgtgg acgccgtacc ccagcaccgt tccgtgaacc tcttcctgcg aggaggggtg
     3181 gatgcctgct gcgccatgta ctacaacgaa taccacgcca tctggcaggc ggggctcgac
     3241 gaggaccaga tcgagccctt ctttctccgg gactgggggc tcgacttccc cgaagacggg
     3301 ctctacaccc tggcttccac ggcgacgcgc tcccccgacc tggcccggaa catcgcggag
     3361 ggcaccctgg aggggtggcg ttttgcccag gcgtcgccgg acgtggcact ggatctggtg
     3421 atgcgccggg cggaggacgc ggggatcccc gccaaccgcc cccacatggc gtggatgctc
     3481 acccacatcc tccccgccat cttccccgac gacgggggct ccgattcccc gggaaagctc
     3541 tcccgggagg cctatctccg caccgcccga atcctcgagc gccacggcat catccgccac
     3601 atcgacccct tcgagtcctt caccctggac ggagtttccc gtgttcccta gagttcgcac
     3661 cctcgccgga cgcatgatcc tgcttttggt cctggggggc ggaggagtcc tggtcgccct
     3721 gtcgggctat aactattact cttccctgaa aatcttccga gaggagctgc gcctccgggc
     3781 ccaggccacg gtgaaggggg cggcaaaccg ggtggagagt gtggtgcgga gcgtggaaaa
     3841 ggtcaccgag gaactggcgc tggccctccg gatcttaagc cccgacgccg ccctccgcca
     3901 cgccctgctg gcggccaccc tgcgggacca gcccgagatc ttttccatca ccgccgccct
     3961 ggagggatcc gacggggccg tcgccttgct ccaggcgggg cgccgcgccc cggggacctt
     4021 cgtccggccc cgggatcccc ggagctaccc ctacgccacc ttggactggt tcacccttcc
     4081 ccgggacctg ggacgccccg cctggagcga gcccttcagc tccgccgaag ggggcgcccc
     4141 ggacgactct ccggaggagc gccgtcagct ccgggcctcc tacgccgtgc ccctcttctt
     4201 gggccccgga ccgtcggagg ggtttttcgg cgtggtggcc tgcgacgtgt cccttgcctg
     4261 gctttcggcc tacctggcct ccctccccct ggatgggggt tacgcctttt tgctgtcggg
     4321 aaatggaact ttcatcaccc acccaaatca gacctacatc atgcaagaga ccatcttcag
     4381 cgtggcggag gccttcgatg acgccccctt gcgccaactg gggcagcgca tggtgcgagg
     4441 agaaacgggc tttgccgcat tgcataatcc ctccaccacc aaggactact ggatcggctt
     4501 ttcccccgtc cccgccacgg ggtggtccct gggggtgctc tacccccagg acagtcttct
     4561 ggaagccctg gggaaatgga gccgccagga gctgacgctg ggggccctgg ggcttttgct
     4621 cctcgccgcc ctggtggtga tggtgtgccg ctccgtcacg tcccccctgc gggagctcga
     4681 cacggcggtg cagcgccttt ccgccgacaa tctgcacgcc ccgttgccct ccgtggaagg
     4741 ggtggacgag gtggcccgcc tgggacggtc ttttgccatc atggtgcggg atctccgggc
     4801 ccacatggcg gagctccagg ccgccaccgc cgcccgggag cgcattgaaa gcgatctgcg
     4861 catcgcccac gagatccaga tgtccttggt gccccggatt ttccctccgg tgcgggggct
     4921 ggagatcgcc gccctgctgg atcccgcccg ggaggtgggg ggagatttct acgacgcctt
     4981 ccctctagac caggacctct gggccttcgc ggtgggagac gtgtcgggca agggcgttcc
     5041 cgccgccctc ttcatggcgg tgacccgcac tctgctgcgg gggctggccc ccacggaacg
     5101 ggcccccggg ctcctgatgg cccgcctcaa cgacgagctg gcggcggaaa acgacacctg
     5161 catgtttgtt accctctttt acggcgtggt gcgcccctcc acgggggagc tccgctacgc
     5221 cagcgcgggg cacccccttc ctctgcgcct tgctcccgga gagcccgtgc gccctctgcc
     5281 ccgggtttcg gggcccctgg cgggccccct ggggggcatg acctttcagg agggaagcgc
     5341 cacactggcc tccggcgaag cgctcctggg gtacaccgac ggcgtcaccg aggccctgga
     5401 tcccgagggg accctctacg gcgaagagcg cctggtggct tttctcgaag cccaccgggc
     5461 cctggagccg aaggaactga tggaagccct gcggcgggag ctgcgatgct tcgcccagga
     5521 ggccccccag tccgacgaca tcacgctgat gatcctccgg aaaagcgcga cggcagactc
     5581 ctgagcaggg cccccgcatg gcgtttcgtc ccccgccccc cagcaccccc acctctctgg
     5641 agcgccagga aacggcggcg aggacggcgg cggtctcccg gaagtcggag gggttttggc
     5701 tgtcgctcca gggagctcgc gccagatgcc ggagctcgtt ttccagaaaa accacgggtt
     5761 tcgagccatc ggcatcttgt gccagttcgg gaagggccga ccggatcatg gggtaaaaga
     5821 acttgccgat gaagtcctct cagagttcga cgtcgaagcg ccgcccaacg tcgttcttat
     5881 cctgcgcatg ctgtgggtgt ctgtgggtca ccgacctcgc ccccttgggc tggattctca
     5941 cgaagacagc acaacgggac acccaggggg agccccggag gacattctcg ggcggccgga
     6001 gcccgggggc gttttcgaag gggtgctcca ggcaagacgt gcggtggcgg tcgtctgggc
     6061 gtgctgtggc ggcaatcgtt tggacgtgtg gccgcaatct cctgtagggg cgaaaaatat
     6121 gctcgcccct acatatttca tccggtgttg tcgacatgaa ccggggaaag cccccaacgc
     6181 atttcacccc gcgtcgcatg acgcggggct tggggattgt ctctccgtcg tgcgttccac
     6241 tatactcttc ttgcaaaaaa cccggagcgg gggcacctcc tgttccggtt acgaagggtg
     6301 gcgatgtttg tgcgcatatc gcggtgtggc ttcctctttc ttcatcccgg cgaaccggga
     6361 gatctccggt ggcgaaagac ctttgcaaca ccggaattca ccatggaaat ccggggcgtg
     6421 gcctcccccg aggaggggga aacggcggcc cgggagctgc gagacggcgg ggtccagatc
     6481 ctcgacctct gcggaggctt cgacgatgag ctcaccgccc gcatcgtggc cgccgtggaa
     6541 ggaaagatcg ccgtgggggc cagacgcccc gccgccggat cccccccacc ccccgacgct
     6601 ccccgacgat atggcttcat cttcaaaggg cccggcatgg tcccggagca ttgccaggag
     6661 accttcgagg cgccggggta tttcctggaa gtccggggcg tgtcctccgt cgaagagggg
     6721 gaaatcgcgg cccgggagat gggcgaccgg ggcgtgacca tcctggggct ctgcggaggt
     6781 ttcaacgatg agctcaccgc ccgcatcatg gccgccgtgg ggggaaaggt cgccgtgggg
     6841 accgtacgct atgcccccga ggaggcggcc aagatctcac acctcctagg gctccccgag
     6901 gcgtgacctc gcgccctgaa acccgccccg ccgccgccct ccgcaagatg aaacatcacg
     6961 gtggagggcg ctctgggggg caaagggtgt tataattctc ctaacgtcac cgtgccgagg
     7021 gcacccttga ggctccgcca acgggcaccc cggcggtggt cgtggttccg ctcgtcgaaa
     7081 aaagtcggcc ttccgtaaaa ggacttacgc acccatcgcg ggaagggggc gatgtttgtg
     7141 gcatctcggg ggggaaggtt ccgcgtgagg ggcctcgggc gccttttcag gggggtggcc
     7201 cttttgtttg tttgggtttt ggggcccgcc gccggggcaa cgccccctct ccgagtgggg
     7261 atttacgaaa accgccctaa ggtcttcacc gaggcctcgg gtgtcccggc ggggatcttc
     7321 gtggacatta tcgaagccat cgccgatcgg gaaggatgga cgctggagta cgtgcggggc
     7381 acctagcgag aggggctcac ccgtatggcc tcgggagaga tcgacctcat gccggacgtg
     7441 gcctacagca gccaacgggg ggaactatac gcgttccacc acgaacccgt ggtctccgac
     7501 tggtttcagc tcttcgcccg accttcggca aagatccgct ccgttctgga tctggagcgc
     7561 aagcgagtgg cggtgttgga agggtccatt cagcaggaat ccttctgcaa gaccgtgcag
     7621 gatttcaaca tctccatcga cctcataccc tttccctcct ttgtcgaagc cttcgtcgcc
     7681 gtgacgagcg gcagcgccga cgcagtcata tccaaccgat tcaacgcccc cttcctccgc
     7741 caggagtatg ccctggagga cacggcggtg atcttcagcc ccacccggat atatttcgcc
     7801 gccaacaaag acgccgatcc cgccctgttg gacaccctag accggaacct ccagcgcctc
     7861 aagaaggatc cgcactcggc gtacttcgac gccctccggc ggtgggccgc cgaagagatc
     7921 ccccaagaac tcccccactg ggtccttgcc acaggggggg ggacgttgtt ccttgccctt
     7981 ctggcgctgg tgaacatggt ggccctgaag cggctggtga acgccaaaac caaggagctc
     8041 cgggccctgg cgcaccagct cggcgccgca gggcgccagt ggcgcgccac cttcgacgcg
     8101 gtgcaggacg ccatctgggt gctggacgta gaaggaagaa tactgcactc caacaccgcc
     8161 gcccatcgcc ttttcctcac gtcccccgag gacagccggg aactgcgctg ttgggaggcg
     8221 gcccacggcc gcaacgggcc catcgatgac tgccctctga tggtctcgaa aagaacccta
     8281 cgccgcagta tcggcacggt ggacgccgga gaccgaaaac tctcggtgac ggtggatccc
     8341 atctgcgacg aggaggggca gctcgcgggg ttcgtgcacc tggtgagcga cgtcaccgac
     8401 aaaattcggt cggaagagga gctggagcaa taccgacaag ggctggaaga tctggtgcgg
     8461 caacgaaccc gagagctgga agacgccaat gccgaccttc tccgggcaaa aatggcggcg
     8521 gaatccgccg ataagctcaa gtccgccttc ctggccacca tgtcccacga gctgcgcact
     8581 ccattaaact ccatcatcgg cttcaccggc attctgctcc aggggctggc gggctccctg
     8641 aacgaggagc aggacaagca gatgcgcatg gtgcagggca gcgcccgtca ccttctggcc
     8701 ctcatcaacg acgtgctgga catctccaag atcgaagcgg ggaggatggt cctcgcccga
     8761 gaggccttcc ctctggcccc catggtggaa aaggtggtgc agagcgtagc ccccacggcc
     8821 caacagaagg ggctcaccct ctcctgccac attggcgacg acgtgggcga gctggaggga
     8881 gatcagcgcc gggtggagca gatcctcatg aacctcctca gcaacgccat caagttcacc
     8941 ccccaggggc gcgtatcggt gacctgctcc cgggaaaacg acacggtgac cctggcggtg
     9001 cgggacacgg ggatcggcat cggcgccgag gacctcgcca ccctcttccg tcccttccaa
     9061 cagatcgaaa cggggcgaac ccggcagttc gacggcaccg gcctggggct tcacatctgc
     9121 aaacgcctgg gggagctcat ggggggatcc atcacggccg aaagcgtgcc ccaagtgggc
     9181 agcgtcttca cgttgcgcct tccagcgaaa ggagcggagg tccatgcccc cgacgaagat
     9241 cctcatcatt gaagacaacg aacagaacat gtacctcatg cgcttcctgc tggagaagca
     9301 ccggttcgac gtccgggagg cctgggacgg gaaagagggc atcgccatgg ccctacggga
     9361 gccccctgac atgatcctcc tggacatcca gttgcccctg atggacggat acgccgtggc
     9421 ccgggagctc cggtcccatg aggaaacttc cgccgtgcct atcgtggcgg tgacttccta
     9481 cgccatgccc gacgaccgga gcagatgcct tgcagcggga gccacggatt acatcgaaaa
     9541 gcccatcgac ccggagacct ttgtgaggca gatcagcaat catctgcccc gcgaggcctg
     9601 accccggggc cgcccccgga gggagacgga gggatgaacg tcctcatcgt ggacgacaag
     9661 gcggaaaacc tataccttct tcaggccatc ctcgcgggcc acgagttttt tgtggcctct
     9721 gcccgcgagg gaagggaagc cctggagaag gcccaccagt ccccacccga cctgatcatc
     9781 agcgacatcc tcatgccggg catggacggc tttgccctgt gccgggagtg gcgtcaggcc
     9841 ccggagctaa aggacatccc cttcatgttc tacacggcca cctacaccga cccccaggac
     9901 aaacagtttg ccctggacct gggtgccgat cgcttcctga tcaaacccat ggagccggac
     9961 gcgttcatgg tcgaagtgcg acaggtcctg gaggaccgag cctccttccc cggcattgcc
    10021 gtccgccagc cctgtgccac cgaagccgtc atcatgcggc agtacaatca ggcactcata
    10081 cgaaagctgg aaaaaaagct ggacgatcgg gagcgtgatc tggcactcct ggcggagagc
    10141 gaacgccgct tggacgaaat caaccggatc cttcgagggg tacacctggt gagccacctc
    10201 atcgcccagg aaaaagaacc cgcaaacttg cttcgcctcg cctgcgaagg gttggtgcgg
    10261 ggacagagct ttccatcggc ctgggcggtg cgatttcccg agggtctttc cacccccgaa
    10321 gggttccaga gcggcttacc cgaggcactt tttcggacct ttttaaaact attccatgac
    10381 ggagagctcc ccccctgttg ccaaaaaagg gaggatcgca ccgaaagacc ttgccacgac
    10441 cgttcatccc cctgccccct ggagaaaatt tcgccatcgc agccttggat cgtccatcct
    10501 ttggagcacg aagggcagat cttcggcttc ctggggctaa ccccatcacc catcgccgcc
    10561 tccacccccg aggcattatc cctgagccgg gaggtggccg ccgacctggc cttcgccctc
    10621 cacaccatgc gggaggaaaa ccggcgccga caggcggaag ctcttaggcg cgccatcttc
    10681 gaccacgccc aagacggcat catcctggtc cggggggact cccttcgcat cgtcatggcc
    10741 aacgaggcca tggcccggct cctgggaatc cccgtcgagg agctggtcct tctctccata
    10801 gaagacatcc atgcccccga ggacctcccc cggatgcgtc acctctttga agccgtggcg
    10861 accaaggaac ctcacctttt ctgcgacgtg cccctgaagc gtcgggacgg cagcgtggtg
    10921 cttaccgacg tgaactcttg ccccctggaa ttggagggac accttcactc cctgggaatc
    10981 ttccgagata tcacccgccg caaggaggcg gaagatcgca taacccagct cctgcgcgac
    11041 aaggaactct tgttgcacga ggtccaccac cgaataaaaa acaacatggc ctccatctcc
    11101 agcatgctgc gcctccaggc gaggcaacag aaaaacaccg acataaccca cgagctagat
    11161 gacgccgctt gccgggtaca gagcgcggcg gtgctctacg accggatcta tcggagccag
    11221 agggtctgcg acatttccct aaagagctat ctcccctcgc tgttgcagga gatccttcaa
    11281 tcctttcccc accaagagag tattgccgta gagaccgccc tggacgacac gaaggcctgc
    11341 acgaacttcg tctttggcct gggcatcgtc gtcaccgagc tggccaccaa cgcccttaaa
    11401 cacgcctttt gcggaaggga tcgggggcgc ctccgcctcg ccgccttccg ggaagaggag
    11461 cgctttgtct tggagttcga agacgacggc gtgggcctcc ccgaggagtt ctccctggac
    11521 gccccggcgg ggtttggcct ccacctggtc cagatcttgg tgctccattt tcaaggcacc
    11581 attacggcga agggaaatgg gggcacccgg ttccgcatgg agtatcccct ggcggaggtc
    11641 gtggcggggt gctcctaacc ccctccccct gctccgcttt cttttccgcc aagacttagg
    11701 gccggtaaag ccccggcggg gtttaccggc ccgaggtttg ccctgggacg gggacctcgc
    11761 cgcccccgag cacgaaaatt aagaggctat tctttaatgt acgagcagat atactccgaa
    11821 atgccgtcgc agcggtagga aaatcggctg tttcccggga cgtcgaaaaa ctcccccgcc
    11881 gagaccttcg tccaggtgga gcttccggga aggagcactt cggcggtgcc cgccacgatc
    11941 tccatgtgct ctcggtctcc cgtgccgaag ctgtactccc cggggagaac cgccccgacg
    12001 gtcttgcggg tgccgtcggc gaagagcacc gtgtggctga tcaccttgcc gtcaaaatag
    12061 acgttcgcct tggtcaggac cgttacgttc tcgaagcgct ccgtcatgtt cccgcctcct
    12121 tcaagtgggt caagccttgg gggatgggct ccccctcctt cggagtatat ctcactcttc
    12181 gccctttcca aacccctaga aaaccttcgg gcgtcacgga ggggggcaca gaacggggca
    12241 aaacatcccc ccagcctccc gtccctccca tcaaagggcg aaggcccggg agtttggggt
    12301 tataatggct ccagacggga tcttttcgca ggaggaatgc ccatggcccc gcgccgaccc
    12361 gggcccgggc cgcaaggatc ggcccgggaa tccttttccc tttacaacga cgtttccccg
    12421 gtgcggggtc ggtttatctc tcgccgggat cgcttcaccg tgcgctgcgc ccccgagggg
    12481 agcgaggaaa gctgggctca tctgcccaat cccggcaggc tctgggagca cctgatcccc
    12541 ggggcgaccc tgcgcctcct cccggcccgc cgggggggca tgccctggcg ggtggtgggg
    12601 gtggacgccc ccgaaggcac ggtgatgctt gacaccatcc gttccaacga ggcggtgcga
    12661 tggctcctgg agcaccgcct ggtgccgggc ttcgaggagg cccgggtggt gcgctccgag
    12721 gcggtccacg gcgacaaccg gttcgatttc gagctggagg aggggggcaa accccttttc
    12781 ctggaggtaa aaagctgcac cctcttttcc ggaggggtgg ccatgttccc cgacgccccc
    12841 acggagcggg gacggcgaca cctggaaggg ctcgccgctc tggcctcccg ggggaccccc
    12901 gggggggtgc tcttcctcat ccaatccgcc gccgtgcggg gctttttgcc cgatttccac
    12961 actgatcccg ccttcgccga agcctttgcg gcgtgcgccc cccggctgcg ggtgcgggcc
    13021 ctgggggtgg actggacccc ggagctctcc ctgcgtctgc ccgggcgccc cgtgcccttc
    13081 cttcgggata ccctgctgcg ggaaaatcat gaccgaggta cgctcctgga agccctttcc
    13141 cccaaggctc ccgtcccctt cgacctcgga gaaaggggca cgatgttgga acccggacac
    13201 tacgtctacg gggaggacct cccccagggg ctccaccagg cggcgcgccg aaggcgaagg
    13261 ggttttgccc tccgcctccg gggcgccccc gaccccggag cttccaccgc cacccccctg
    13321 cccatccgga gctccacccc cctggccgag gaggcgttcc agaggctggc ccccctggga
    13381 gaagtcctcc ttcgccaaac tgaccccggg agttttctag tgcgcctgcc catccccccc
    13441 ctggcgcacc gcgccgtggt ggacctgctc tttttcctcc gcatgggacg cctcgctccc
    13501 ccggaggaag gcccctcggg gggctttagg taatggattt tgatctttta ggagggaagg
    13561 ccatggacaa ggtgatcctg gtgacggggt gctcgtcggg gatcggaagg catctggtga
    13621 cggccctggc ccaggcgggg taccgggtgg cggcctcggc tcgacggccg gaaagcctcg
    13681 ccgacctccc cgccgccatg acgctgccgc tggacgtgac ggccctcgag ggggcgtccg
    13741 aagcggtgga cgcggtgcgg cgacgactag gccggctgga cggcgtggtg aacaacgcgg
    13801 gcctcgcctg ccggggagtg ctagaagagc tctcggagga ggccctgcga catatcttcg
    13861 aagtgaacgt cttcggcccc ctgcgccttt gccgggcggc cctccccttt ttccgggagc
    13921 agggatcggg gatcatcgtc aacgtaggct ccatcgccag ccgcatggtg atccccgtaa
    13981 acggggccta ctccgccacc aaggccgcct tggaggcctt caccgacgcc ctgcgcatgg
    14041 aggtggcccc cttcggcgtc cgggtggtcc tcatcaagcc gggaaacatc cgtacctcct
    14101 tcgccgaagc ggccgcagag cgttcccgaa agaccctgga aaacccccga tccccttacc
    14161 gggagatcta ccggcgctac ctcgccgagg ccaccaaagc ccggcgccac gacccgggcc
    14221 ccgaggtggt ggccgaagcg gtcctcaaag ccctggaatc cccttctccc cgggcccact
    14281 ataccgtggc ggccccttgg cgcttccggc tcctcatggc gctgggggga aatgggcgca
    14341 acaccatcct gggtcacgtc ttccggggcc gggacgcccc ccctcgaaag ccctcctaaa
    14401 gtttttcagg ggaggggggc gaagggaccc ctcgcggtag aatggccgaa ccccgcgccc
    14461 cgacggatcc gccggaaggg ttttcgtcaa aggggaaggt cgagggacag aagcgacgac
    14521 ttggacgact tgaaggaagg gaataccatg gcactcagca acggaaaagc ctttcccccg
    14581 caagcccgag gccaccgggt gtttctggcg ctgggcacca acctcgggga ccgccaggcc
    14641 cacctgatcc aggccctaca gtacatcaaa agccgccttt ccctggaggc cgtgtcgcct
    14701 ttttacgaga ccgaccccgt ggggtacacg gcccaacccc tttttctgaa cgccgtatgc
    14761 gagggataca cctccctctc tccccaaaac ctcctcgcct tcgtcaaatg ggtggaacgg
    14821 cacatggggc gcacccccac ctttcgccac ggcccccggg tcatcgacgt ggacgtgctc
    14881 ttttacgacg acctcgtcat gagctccccc gggctggaaa ttccccaccc ccggttggcg
    14941 gagcgggcct ttgtcctggg acctctggaa gacatcgccc ccgattttgt ccatcccggg
    15001 cttaaaaaga cggtgcgcga gctcgccctc caggtggggc ggtcggggat ccgccccgcc
    15061 cctcgggccc ttcccttcgc gctggagggt gacgtccagc aagggcaccc cgaggtgccc
    15121 ctggggctct cccgggtggg gatctcgaac ctccggcgca tcgtccgcct ggggacggag
    15181 gaccgggtgg ccctcttcta cgccaccatg gacctttttg cccaccttcg gggagatcgg
    15241 gccggggtac acatgtcccg gttcagcgaa atactggagg agatggggga agagatctcc
    15301 ctgcccccca cccccgacct ggaatccctg gcggagcgca tcgccctgca gatcgcccgg
    15361 cgccaggagg ccgcctcgtc ggaggtgcac atccgggccc aattcctggt gcgccaggag
    15421 accccccttt cctcccgccg atcggacgag ctttacacct ttttggggta cgccgccagc
    15481 gacggcgtca ccgtccgccg agccgcagga gtggaggtgg atgggctcac ggcgtgcccc
    15541 tgcgcccagg acatggtgcg cggcgcggcc ctggcgcgcc tccgggaggc gggctatgcc
    15601 ccggaagagg cggagcgcat cgtctccctg gtacccctgg cgacccataa ccaacggggg
    15661 agggggacgc tcatcctggg gtgcgacccc aagatcccgg cggaacacct ggtggccgtg
    15721 gtggaggccg ccatgagctc cgagacgtac gaactactga agcgccccga cgaatttttc
    15781 gtggtggaga aggcccatcg gcggccgcgc ttcgtggaag acgtggtgcg ggagatgttg
    15841 cgccacgtgg tggaagcctt tccggacctc cccgatgaca ccttcgtgct ggcccggcag
    15901 gagaacctgg aaagcatcca ccagcacaac gccttcgccg aggcgagcgc cctcctgggc
    15961 tccctgcgcc gggagctcgc cggaggcccc cccgcctcgg ggccatcgct ggagggctgg
    16021 ctccgggggt agacccaaag ccctagtacc cgtcgtaacc cccgtcccgg tagcggttct
    16081 ggtcgatcat gattcccatc atcatgagct gcatcaggag ctcatattcc ctaggccccc
    16141 ggggacgcct ccggtagatg ggaggagggg gcacgatggg ccggggccga gggtagggcc
    16201 tgggagggcc ccaccggtaa atcgggggag ggacggggta ggggtaggga tagnnnnnnn
    16261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntagggat agatccccgg agggaagtcc
    16381 cgatatcgag gaggaggggg catgacggga tggggacggg gctcgtacgg agggaaaccc
    16441 cggtacccgg gccccggaga aaaccattgg gccccgcctt tggccgtcga acccttggag
    16501 cccccttcgc cttcagccca cgccccgctg gcccccagga gcaaccccag gaccacaaaa
    16561 accaccgcat attttttcat ttcgacccac ctccccacaa cccatcgcgc cgaggtccga
    16621 aggctcggcc ccggatcatc cttccacctc cgcccaactc cgcccgggga gcgcttcccc
    16681 tgtaaaaaat gagggacgcc acgccccggg cctccgagtc gttcgtccct gcactataac
    16741 agcatgactc ctaaaaaaaa ctccgtaatt cttctgataa gctgaaacat caccccgccc
    16801 tcggcgccgc ccgaggatcc tccgacaaca gggcgcccca ggggatcctc agatatcaaa
    16861 agccttaggc gagggctaaa ggaccaccgg ccctgacggg gcggatcccc ggaattctcc
    16921 ggcgctcctc ggggcgacct tcggccaagc cccggatttc ggacttttta ggcgcggcta
    16981 ggcgtgcaaa aaggccgaca gggccccgag ccctccccgg agcgtttcgg tgtggggagc
    17041 aaagccgatg cgggcggaac agtccgagaa ctcgaaacac gatccgggga cgagcatggt
    17101 tcccgtagcg tcaaagagct cccgacagaa ggcctcggac ccctggtctc gctgaaagcg
    17161 cagcagcgcc gtggttcccg cccggggagc ggtccacgac accaggggct cctggcgaac
    17221 ccactctccc agcaccgccg cattggagcg caccagggcc aagtttcgct ccagcacccg
    17281 gtctcggtgc tccagcgcca gggccgccag atagtcattc acccggccgc aggagatggt
    17341 ggtgtagtcc cggtgccgag agcactcttc cagaaactcc cggggcgcca cgatccaccc
    17401 cgtccgaagc cccgccaggg agtagacctt ggagaggctc cccgtgccga tggcccgggg
    17461 atagaggtcc gtgaacgatg gcgcgccgta ggccccctgg tgctctaacc ctcggtagat
    17521 ctcgtcgcag aggatccacg cccccacccg gtccgccacc gccacgattt cccgcatggc
    17581 gtcttcgtcc agcgaagccc ccgtggggtt attggggtag ttcagcacga tgaggcgcgt
    17641 gcgatgggtc accgcccgcc gcagttcctc cacgtcgggg cgaaaggcgt tttcccgccg
    17701 ggcgcgtagc cgggtaaccc gggcgccaaa ggattccggc agggcgtaga gttgctggta
    17761 ggtgggaaga agggccacca cctcgtcccc gggctccacg aagctccaga gggcgatgaa
    17821 attcgacccg atacccccgt tggtgatgag cacttcctcc gccgagcgcc ccttgtagag
    17881 ggatgccacg gcgcctctca ggcgctccga tccggggata tctccgtagg taagatggat
    17941 ccgtcgcagg acttccaggt gcctttcctc gtcgccacaa agggccaaga gctcgtgcac
    18001 cgtcatagat tccacgcagg tctccgctat gttgaacttc gcccgggtct cgtactcgtt
    18061 catccagatt tctactccga agtccgcgat cttcatggac gatctccctc ccctctcgct
    18121 gtgcgggacg atgccccctt ctgccacttt ctttcccgca acatcgccgc atctaccttg
    18181 ccctggagcg acgcgtgggc ccggatgagg gcctccgccc cgtcggcgtc ccgccgtatc
    18241 atcgtcgcca cgatctctcg gtgctcccgg gccgaggcga tgagttcctc ctccggcagg
    18301 ggcaagagta gggcgtagac ggcggtgcgc cgaaaaaaaa cctccagggt ctccgccaac
    18361 acgggactcg ccgccaattc ggcgatgacc cgatggagcc ccaacctctc cggaaagagt
    18421 tctcccagcg tgccaagccc ccgggagggc ccttcttcct ccaaacaggc ctccagccgg
    18481 gcctctccca ggggatcgag gcgcgccacg gccttccgca ccgccaaggc ctccagatgg
    18541 ccccggattt ccgccgtttc cagcacctcg gcgggggtgg gacataccag ccgggcccct
    18601 ccccggggca ccatctccac cactccgtcc gccgcgaggt ggcgcagggc ctcccgcacg
    18661 ggggtgcggc tcacacccaa ccgggaggcc aaggcctcgg tgggaagacg ctcccccccc
    18721 ttaaaggtcc ccttcagcac gagctccaga atgccccggc gagccccctc caccgccgtg
    18781 tctccgcgcc tttgaggggg ccggtctctc cccagggacc caagggccgt catgacaccc
    18841 tccgcttcag gatcgccagg aggcgccctt catcgatgtt tcccccggag acgatcaccc
    18901 ccacccggcg cccccggagg tcgagcttcc tctccaggag gactcccacc gccgccgccg
    18961 ctcccccttc gatgacctgg tggtgcgtgc ggtgggtcca ggcnnnnnnn nnnnnnnnnn
    19021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19081 nnnnnnnnnn nnnnnnnnnn nnngatggtg tccaggtcgg gttcctggag gaacatctcc
    19141 atccccaccg tgccttggcc cgccaccacc cagggatcct cgtagggaga gacgtagatc
    19201 cgcccctccc ggcggcagat ctccagggcc cgagcctccg tgtcgtcgta gagggcgccg
    19261 aagacctcca gggtgacgaa gcctcccccc agctggcaaa tggcccggcg cttcacctcg
    19321 gggcacccct cgggaacaaa gatccgggcg tggacctcca aggcccgggc cgccaccgcc
    19381 actccctggg catggtttcc gctggaggcg gtccccacgc cgcgccaccg ctcttcttcg
    19441 gtcagggaga acatgcggta cagggccccg cggatcttga acgaatggca cttctgctgg
    19501 ttttcccact tcaggaacac ctcggccccc gtgatttcgc cgaggtcagg agaaggctcc
    19561 aagggcgtct cgttcacccg atggcgcaaa aacttgtggg cccgcactat ctccgtgatg
    19621 tcgggccatc cccgctcttc catctctggc ctcatgactt cacctcccta aaaccgatcc
    19681 ccccgcccga tcctcctccc ttcgacccct tcacggaagc ctccggaggc aaagggggca
    19741 aggggtcgcg cccctcactc gacaagaagc tgtcccgccc ccaactccgc cgcccgggcg
    19801 agggcccgag aggcccccgc caagtcctgc aacgccatgc ctgtgacgtc aaaaagggtg
    19861 atctcctcct ccgaggagcg ccccggcgct cgccccagga ggacctctcc gatctccctc
    19921 accgatccct cttccagaaa gccccggtcc cgggcatggt gacactcccc ccgggagaag
    19981 gcctgggccc gatcgtcggc aaagacccgg gcctcttcca gcaacgaggc ctccacctcc
    20041 tgcttttcgg gcatgtccgc cccgatggcg ttcacgtggg taccggggga gatccagcct
    20101 ttccatagga tgggagtgga ggcgggggtg caggtgacga tgagctccgc ctcccgagcc
    20161 gcagcgggca cctcgttcca ccccaggggg cgcagggaaa tctggggaaa aaagggcgcc
    20221 atatctccca cgaagcgccc caccgccaag gcgttgcgcc ccgccaccgc cacgtccctc
    20281 acgtggggaa gcgcggcggc gatccccgcg agctgggccc gggcctggct tccggtacct
    20341 aggatgagcc ctcggcggat cccggggcgg gccagaaggc gggcccccac ggcccccgcc
    20401 gcaccggtgc gaagctccgt gagagaggcc ccatccacca atcccagggg atggcccgtc
    20461 tccacgtcca tcaccaccac caatcccgcc agggcggaaa gcccccgctc cacgggatta
    20521 tccccggcgt agcccatgac cttgaggccg aaaaaccccc ggggaaaacc ttcttccccc
    20581 tcccgcaccc agccggactt gatgtccatg tcccggagct cgggctcgaa gacctgggaa
    20641 attacgggcc accgcacccc ctggccggaa acatgccacc ggtaggcccg ctccacgcag
    20701 gccatcgcgt cttccatgga caaaagggcg cgaagatctc gggcgttgag ataacgcatg
    20761 ccgtaactct tcacctttgt gacacctcct cggggcctcc cggcgctgca aggcccccct
    20821 caattccctt taggggtggc ggagcgaaac gattcggggc ataattggat ccagtggatc
    20881 caaattatac tccacctctc ctctccgtca aggggaaatc cccccttctc caggagcgcc
    20941 caaaaagagg cgtggcgctc ccatcgtggg agtgatatcc tgtgaggagg tcccacgcct
    21001 ttctggaaga actgcgtcgc cccgggatgg ggaccctctt gcgccaggac gcctgagtgc
    21061 gccgccgatc tccgctggca gcccgtcttc cggtgacatc aaagggccgg gggcctctgt
    21121 cgccttggag atccggcccg gggggcaagg atcctggggg acttccggga gagggggaag
    21181 tccccctccc cgggtatcgt tgtgttttta aggaggacat gccatgaaaa acgccgtgat
    21241 cgacgtgatt cgctcccgcc gcagcgtgcg ccgctatcgc cctgacccgg tccccccgga
    21301 ggccctggaa gccgttctgg aggccgccct ctgggccccc agcgcctgcg atggccaacc
    21361 ctggcacttc accgccctcg tttctcccga gcgcattgcc cagatagacc gagaggcctg
    21421 tgccttcctc gcccgggctc ccctggattg ggcggcggcc atggggcgcg ccgctaactt
    21481 ccgcatcttc tacggcgccc cggcggtggt ggtggtatcc ggccttaagg acgcggagtt
    21541 tcatacggcg gtggattgtg ccatcgccgc ccaaaatctg gtgctggcgg ccgagtccct
    21601 ggggctctcc agctgctgga tcggcctcac ccactggtac ttccagtccg agggggcccg
    21661 acgggacctg gagatccccg aaggctacga gccccacttc gccatcgccc tgggttatcg
    21721 ggaaggcccc gctccggagg aaccggagcg ccgagggcac cccgtgacgg tcctgcgctg
    21781 acgcccggcc ctcctgagcc ctcggaccgc ccaccctcgc ccccgcatcc aaagttcgag
    21841 gcgctcctcg ggggtgaaaa ggccggggcc cggggggcca tcccttccgg tcaaaagacc
    21901 ctacatacgc cagaaaagag gagaccccat ggatcggtcg acgtggtggc aaggcgtaaa
    21961 aagcatggtc cccctggcgg tgagcatcgc gggatgcggc gtcgcctggg gaacgctggc
    22021 ccggcagggg gggatgaccc ttccggagat ctttctcatg tcctccgtgg tcttcgccgg
    22081 agcttcgcag ttcgtggcgg tgggcatgct cggcgccgga gcgggcatgg gggagatcat
    22141 cctcaccacg ctgatcttga acctccgcca cctcctcatg ggagccgccc tggcccccta
    22201 cttccggcgc ctcgccccct ggcgcaccgc cctcttggcc cacggcctcg tggacgagac
    22261 ctttgccctg gcggcggaaa gattccgcac caccggaggt gacgcggcgt tttttctcgg
    22321 cactggtacc ttggcgttcg tatcgtggaa cgccgcgacc cttctagggg ccttcgtggg
    22381 ttcggggacc ctgaaccccc gggactacgc cctggatttc gcctttttgg gggccttcac
    22441 cggtctattg gtgccccaga tcagaagccg aagcaccctc ttggcctttc tcgccgccgc
    22501 cgccaccgcc ctgggggggc gcttcgtggc ctcggaacgc accgtggtgc tcctttccgc
    22561 cgtggcggcg gcggtggcgg gggtgtgggg agaacgccat gcgaactgac atggtgctgc
    22621 tcatcctggg catgggggcc gtcacctacg ccacccgctt cacctgcctc tttctggtgg
    22681 gaaggtacgc cctgccccct ctggtgaccc gggcgctggc gtacatcccc atcggcattc
    22741 tcacggcctt cgtggtcccc gccctcttct tttccgcccc tggcgacggc gccggaggga
    22801 cctccgagat gctctggggg gggctggcct ccgccgccgt ggcggcccga tgggggcacg
    22861 tcctgggagc catgctggcg ggcatggcgg tggtggtgct cctgcgctgg agcttttaga
    22921 cgccccatcg tcgcccaccg gagccccccg gggcggcagg gggctacccc ttgatcaccg
    22981 ccaccgggcg gagcctcgcc accttgaggc tcaaccccgc gccctgcacc acgtccacca
    23041 cgtgggacac gtccttgtag gcttcggggg cctcctcgcc gagggtgtgg aggttctccg
    23101 ccatgaccga aacccctcgt ttcgccagag accgcaggag atccttggcc cccgtcctct
    23161 tcagcgcctc ggttcggctc atggtgcgcc ccgcgccgtg acaggtggag gcgaaggagc
    23221 tttcctccgc ctggggcgtt cccacaagca cgtagctcgc cgtccccatg ctgccgggga
    23281 tcaggacggg aaggcccgcg aaggcccggg tcgccccctt gcggtgcacg cagagggtcc
    23341 ttttccggcc gtcccatcgg tgcgtttcca gatgcgccag attgtggctg acatcccaga
    23401 cggtatacag gggctcccgg ggaaagaacg ccttgaaaac gcgccgcacc gcgtcggcga
    23461 tcacgtggcg attcgtcatg gcaaagttcg ccgccgcccg catggccccc atgtaccgtt
    23521 gcccctcctc cgacgacacc ggcacgccgc agagctggcg gtcggggacg tcgatgccgt
    23581 aatgtcccat ggcccggcgc atgatcttca gcgcgtcgtc gcacacctgg tgccccagcc
    23641 cccggctgcc gcagtggatc atcaccgcca cacatccggg aatgagcccc aggcgggcgg
    23701 cgtccttttc gctgtagatc tcgtccacca tctggatctc cacgaaatga tttcccgatc
    23761 ccagggtacc cagctgggtc cggccccggt ctttggcccg ctccgacacc tctccgggaa
    23821 gggctccctt gagacatccc ccctcttcgg tgtgctcccc atcgccctcg gtgccaaaac
    23881 cgtgcttcac ggcccacgcc accccgcctt ccaagacctg gtcaaggtct ctgggagaaa
    23941 ggggcttgcc tcctccggag accgcccccg aaggcacggc ggaaaaaagc gcgggaagga
    24001 gcttgtccac cacccggcca aggtcttttg ccgcgatgga ggacgcgagg aggcgcacgc
    24061 cgcaggagat gtcgtacccc acgcccccgg gactgatgat cccctcttcc gcgtcgaagg
    24121 cggcgacccc gccgatggga aagccgtacc cccagtggat gtccggcatg gcgaagctgt
    24181 atcccacgat gccgggaagg gtggccacgt gggccacctg caggatggcg ttgtcgtcct
    24241 cccgcaggga ggcgatgtac tcgtcggcga agatcatgcc gggaacgcgc atgccgggca
    24301 ccgcccccgg atggagcagg aggcgggtgc cgtcgatccg ttccaaaaac tttttcatgc
    24361 ggacatccct cccgtcacag atccagatag agccgcagga aggggtgggg agagctctcc
    24421 agcaccatgc ccccgtacgt cgccgccttc accgcgatgc gcccccccgg cgaaggaaag
    24481 agaagtccct cggcgacgag ggcgggggga tggggcgtca cggtgaggga ggtggggtgg
    24541 aaaaagaccc cttcgctctc caggagaaaa aggagctcgt ggagccaaga gaccaccagt
    24601 tcctcaagat ccagggtctc cagtcggagg acgcggctct ttccggcggt gccctccggg
    24661 ggaagggggg gagccccgtc gaggcaaagg caataaaaac cctccgccgc ctgcagaaaa
    24721 agatcgtgcc acgaggtggc ccacacctcc aaccccgcat cggcggtgtg atcgatgatt
    24781 ttccatccca tggggctccc tccccgaccg gctccaggta aaatgcctcg acgaaaggct
    24841 tctcccccct cctcgcgccc ttgggccgag gggcgcgggc gttgcccccg gaaacgtctc
    24901 cgggaaacgc cttcccctgt cgctttcccc gtcggcggaa tttttttcgc cagcgtcggt
    24961 cgtcgccggt ggatggggcc cacggcctct cgccgggccg aggggagatt cctacgccgt
    25021 cgccaaaatt cccccttctt cctaagcggc atcctgggga gcgccgcgat ctttgcgttg
    25081 cgccgggccg ccgggccgag gggcgggaaa ttttccccac gccccaccgc cccgtggccg
    25141 gaggcttctt cggcgccctc tcaccgtcgg gcccgttcgc agatttcttc ttccgtcagc
    25201 ggggcccggt ccgggtgatg cgagaggcac aggggcacgc gcacccggcg aggcgccccc
    25261 cggggcgaag cgaattgaaa ggccccgctt tccagcgtgg cgatctctgc ggcaacgctc
    25321 ccccgatctt cgaggaagga tctgatctga tcgatgatgc ggggggtatt cgcccgacca
    25381 cagaaccagg tgctcatgtt cgacagggat ccgccgtcga ggtctttagg gttttgggtg
    25441 gccaccacca gccccagacc gtactttcgc ccctgggccg cgagacgctg caacccctgc
    25501 ttacaggcgc tggtgcgcac cgaagggaga aagtccttgg cctcgtccag cacgatgagg
    25561 ccattcatgc gccccggggc ctggatgggg ggatggcggc gcgcccaggt caggagggac
    25621 aggaccacgt gccccaccac gatctgacgg ttccggtccc ccggaacccc cacaaggctc
    25681 accactgaca cgggggtgcg ccctccttcg agggggcgtc cccacaggat ctccggatcg
    25741 gccaagggcc cttcgccgaa gaggacgggg gcggtgaaca tggccgattg caggttgtcc
    25801 gccgcctccc gggccagggc ggaagcggcg gagatctccc ccgccgtccc ggggggaagt
    25861 tggcgcagaa accccagaaa ggcgtggatg tcccccccat cgccctgggc aaaggtcttc
    25921 accgccgccg ccaggacccc cgccatccgc ttcgccttgg ctccgctccc caccaccgcc
    25981 agatccgtca gcatatccac ggtcacggtg gtcgccagct ggaaggcgtc ggcatcgtcc
    26041 cgcagcgcgg ggaaatcggg cagcccccgc agggcgaagg ggcgcccccg gtcgcatccc
    26101 ggagtccaga tccggacctc cgccgtctcg aaataccgct ccgcccgaag atcgtctccg
    26161 ggatcccatc ccttgggggg gtcgggccag cggtccccca gctgggccaa atccccagcg
    26221 ctgtcgaaca ccaccgaggg aatgcccgcc aacgccgcct cttccacgag gcgcttgagg
    26281 agcacggtct tgccgctgcc gctgccagcc aacaccacca cgtgccgggc aaggtcatct
    26341 gccgaaaggc gggcccgggt ggtctctccg gggtccccca ggaccacgac gggggcgtca
    26401 tcggaagagt gatttctcgc ctccggaagc tgtgacacgc cctctcccga ccctccgtcg
    26461 cctccgccga tcatgagccg cagaggggcc agggattcca gggaactcaa gggtttgcgc
    26521 tcccgcagcc agactttaaa ggccccctct tcttccggag cggaggctcc ggagagttcc
    26581 tctcccatcc tgaccaccgc cgccagggtc cggaggtcgt cttcgacggg gggcatgaag
    26641 actcctcccg ccttcaagaa atcctccgtg atccgagggc ccaccttacc ccccaggggg
    26701 atcccttcgg gacggaggat caccaaccgc cgccgggaga ggtgccgggc gatgcccgat
    26761 tcgaccatcg ccgcctgcag gcggtggttg taggccgtat gattttcccg gagcagcacc
    26821 cggaggcaca catgccactc cgcatcgact cccccggcgc ccgtcaccat gcgcttccat
    26881 cgcaggtgaa tcgccgggca ggagcgtcgc ccccccgagg cgctctccac ctcgaagaag
    26941 gtattttccg gggtttcgag ctcctccacg caccgacgac accctgcggc aaagagctct
    27001 cccagggccc cctcggcccg gtcgtcgtcc tccaggtagg gggcgatgtc gaccgaggcc
    27061 cggagctcct ccaggcggcc atccagggcc tggaatcgcg acgacacggc ggggagggcc
    27121 tcccgaaagg ggtccgtctc cggcgaccct agctcgcgga cctcgccgtg ggcgaggcag
    27181 gcatcccggt ggcggtggca ggcgcgcagg atctcccggg gggagtaggt ctccacagcg
    27241 gagaaaaact ccggggcaaa gggccaggtg gagtaggggg gagagaagct cccccgggcc
    27301 gcgtcgagcc tcctttccac caggtcgcgg gcgatggaac tctcggagac gggaaggagt
    27361 cgatggggct ccgtgtaccg gtcggcaaac gaggcgggaa cgagcttttt caggtggtcc
    27421 cacgtatcgc cgaagcaggc caccaccgtc agggtggagg aattgaggcg gtcccgcagg
    27481 gctccaaagc ccttgccgat gtcccccagg accaatttag ccgttttgcc ctcttcgcgg
    27541 tcgggggcgt ggcgtgcggc gtccagcagg ggaaagcagt tgtcgagctg gtcgaaggcc
    27601 atcagagtgg gccctcgaaa gctcaacatc ctaaagatcc cctggatgag atgccccgga
    27661 tcgggctcgc actggacaag gccaagctgc atgccgtcga agtcgtcgac cttactcccc
    27721 tggagccacg cggaggcgag gctccagatg cggaggcagg cctcgttatt catgcccatc
    27781 agggcccgga tcacgtccaa cgaaggatgg gtgaagcggg tccggtggcg ttgccacagg
    27841 gcgtccacca tgactttcgt cgccccgcga aggtcgttct cgccgagggt cgccacctcg
    27901 gcggcgtact ccagcccctg ggcaagggtg gcggagcgcc ccgagatgtc ttcggcctcc
    27961 acgaagaggg cggcgatgac gtggcgcagc tggctcactc cctccacctc ccgggtgagg
    28021 gaatcggcga ggccgagcag aagggtatgg tcgaaggagc gcacgtcggt catgtccacg
    28081 tagacgaagg tcacccctcg ctccgtggcc ctccggcgca gctcccccag gaggtgggtt
    28141 ttgcccagac ccgcatcgcc tttgagcacg atccccaaag gggtttcccc cgcctgggcc
    28201 gaggcctcca cccgggccag aatgtcggcg cgcacccgat ggtgtagctc cggcacgtcg
    28261 cgggggtcgt cgaaccagac gctgccgagc tggcgcgtga aggagaaatc cgctcggcgg
    28321 aggtaggcca gatgctccgt agaaggcaag accatcgctt cctttcggtc cgtcgggatc
    28381 acggcatcaa caccacgacg tgcttgtccg aagcaccgat gcgaatcgcc gcccggcggt
    28441 cctcgtcggt gagggtggac acgtcgttat tcgcctccag ggagacgcgc ccctctttca
    28501 gtagtcccag gagcagggcg tccacccggt ccggcgacca aggggccagg gcccccttga
    28561 ccagggccag gggcaggcgg tcgcccccct gggatttcgc caatcgcccc atgacgtcga
    28621 tgatgcgccg gatcggatct ccatcctccg ggggggcggg ggcgaaaatc gacgccaaag
    28681 cccccccctt ttgcacccac ccttccacca ctcggaacag aaaggccagc atctcccgat
    28741 ctccccgggt gagcccccgt ttggtcgcca cgcgctccgc catcgccccc aggtgggcat
    28801 gggcccaacg ccacccctcg tcggtgacgg cggcgtattt agaccttccc cggcgggtga
    28861 tctccaccaa tcccccctcc gtgaggggct tgaggtcggc ggtctttttc aggcttacct
    28921 ggcggtgcag ctccgtggca gagatgtcct ccgcgccccg ggcctcgcgc agcaggagac
    28981 agtagaacca ttccagggcc ttcccctgaa cttccatggc catcccccca aaaagtcatc
    29041 cccccggaca aagagatccc cggggaatcg tcccccacgc cccccttcga gtcccgcccc
    29101 cggggggacg catggaaatc tacggtcaat catagcgacg ggggccgaaa aagacaaacg
    29161 atagttcttc ccggaggccg gggcatcgag gggggataat gccggcattt ttcccttgat
    29221 gagggtcctc cggattgcta tcatggctcc catcgccccc gggcctccgc cagaacggcg
    29281 ccccatcggg gcagggattt tttccctaga gagagaccta gagagagaaa ggaaggctcc
    29341 catgaccccc atcctcataa gacattacgt ccatccccag cgcagcgagg cggagaacgc
    29401 cacggggctc accctggggc ggctgatctc cgcccacgcg ccgcgctttg ccgctctgga
    29461 tctttccctg gatctggagg tggtgccctg cgacgccccg gaggaccgaa accgcgtcac
    29521 cttttcctat cccgtgccct cggatgacga agagcccccc caggatcggg aacgctccct
    29581 ggaggatctc ttgggtctag gggtcgtggt gtcccccgga gcgccccatc gcaccctggt
    29641 ttacgaaggc caggcctacg acgccattcc cccgggactc ctggcggacg gcctcctgcg
    29701 ggtggccatg gccctcatgg ggggcggagg gtgtggctct tcctgcgcgg ggtgccaggg
    29761 gtgtggggcc tagggacgca ctcaaaggca atttaaggcg attcggctcg gccggggccc
    29821 gcaccggccg agctccggcg aaggcccgat ggtaaggaaa aggggccccg ccacgaaagg
    29881 agagaagatc atggatggag tttggctggt cactcgaggg ggagacaagg gagaaacctc
    29941 tttgggcgac ggagagcggg tgcccaagga ttcccctcgg gtggagacct atggcacgct
    30001 ggacgagtgt caggccgccc tggggctggc ccggagcctc tgccccttcg atgacttggg
    30061 gcaggagatc ctgcgcctgg aagatcacct gggctccgtc atggggtact ttgccgccta
    30121 ccccggcatg gaagagcccg acgtcactct gctggacggc gtggtggaaa gggttcgggg
    30181 cgtggtggga gactccttct ccttcgtgcg tcccggggac tccgccccgg gggcggcgct
    30241 acacctggcc cggaccgtgg cccgccgggc ggagcgctcc gccgtgaaac tctaccgcat
    30301 gaaccagctc tccgaccagg cctatgccta catcaaccgc ctgtccgacg ccatctacgc
    30361 cctatcgctc tgggccgaca aggccagtaa ggaccgcacc gccccatctt caaaatagct
    30421 tcgtgcccct ccgacgaagc cggagaggga ggaaggcctt cggcggaggg gcacgccgtg
    30481 gaacgaatgg gtcgtggcat attttccccg gaagccctat aatggtatgg agccttcagc
    30541 atacaaaaaa cctgacatcg cccgcgcgag gaggtccccc atgccagaca accctctgtc
    30601 cccgacccag aacatcgacc tgcgacagat cgtctacgag aagataaagg aggccatcct
    30661 ggatggcatc atccaccccg gggagcgcct ctccgaggtg gatctggccg aaaagctcac
    30721 cgtgtctcgg acgccggtac gggaggccat tcggcagctc gcggaaacca agctcgtggc
    30781 cctcatcccc cgcaaggggg cctacgtggt gctcctgtcg gtgcaggacg ccaaggacct
    30841 ctacgagctc cggagcgccc tggagtgctg tgccctgacc cacctctgcc agaatccccc
    30901 cgcagaagcc ctgcaggagg tgcgggccgt cttcgacggc ttcaacgatt ccatgtctca
    30961 attcatccac tacgatcgcc aattccacag catcatccga agcggatcgg gcaacaagtt
    31021 tcttaatagc gttttggaca acgtggtgga cctcatccag ctctgccgtc gctacgccct
    31081 ggaggtggtc cccctggcca cctcggcggc ggaacacgtg ggaatcatcg atgccatcct
    31141 cgcccgggac gtggaaaaaa gccaacgcct cctccgggat cacctgcggc gggcctcgga
    31201 atccctggcc acctacctgg aaaaacaccc ggagttctcc cgacagacgc cctgacctct
    31261 cttccatcga tgccgacaaa aaggaggttt ctctgtggaa caaggatggg gttttgtgat
    31321 ctataaggcg gtggcctccc tcgcgctccc cccggggctt ttggtgctgg gactggccct
    31381 tttggggctc ggggccctgc gcccgccccg gcgccggggg ctggggttta cccttttgac
    31441 cctttctgtg gcgatctacg gtatgagcac ccccttcggc gcccaggcac tcctgggcac
    31501 gctggaatcc ccctggaagg ccgatattcc ccctcaggat cggcggtggg gagtactggt
    31561 gctggcggga ggagtgatcg tgagggacgg agagccggcg gagctcaatt cgttcaccct
    31621 ggcccggctg gtggggggat gggaagcggc ccgacagggc ccctggccca tgatcgtgag
    31681 cggcggccct tctctggagg ccccccaggc cccctctcta ggggccctca tggaggaacg
    31741 cctccgccgt tgggggtacg aaggcacggt atggcgggaa gaaacttctc gtaacacctg
    31801 ggaaaacatg acggcctcga aggccatcgt ggtctcggaa gacctggagg gcgtcatcgt
    31861 ggtcaccgat gcctttcaca tgtcccgctc catggccatg gcccggagag ccctgccggt
    31921 ggcggtacgc ccctatcctg tgggctttct ggtggaccgg cgccccatca cgttctggga
    31981 tttcctccca aaccccggga gcttgcacca cagcatgatc ggcctgaagg aatacttagg
    32041 gctctttgcc tacgacctct acggcttcct tttctcgtag ccttctcgtg gccgcccggc
    32101 cagcgccccg tccccttctt ttcccggaga ctcgattaaa acctctcttc tccttcaaac
    32161 agccgggcca tgaggggttc cgcgctcctt ccttccccca gggcctcctc catctcctcc
    32221 gccagacgct ttaggtcatc ccgaccacac tgcgccgcag aggatccgag cctttccgcc
    32281 aggatgcggg cctcttcttc gtttcccgcg acccaggctc gccggagggc cgtgcggatc
    32341 agggggagct cggcggcaaa ggcctcttcc atggagagcc tatcggcctc gtcctggggc
    32401 ggggagacgg ccccgtcgta gcccgtccac tgggccacca ggtcccagag cagcttccgg
    32461 ggcacgggct tcagggcgta gtcgtccatc cccgcctcca gggcttccag ggcggtcccc
    32521 gctccccccg ccgtgagggc caccacgggc acggtgaacc cccggcggcg cagctctcgc
    32581 gtagcggcga tgccatccat gtggggcatg aggatgtcca tgatcaccag atcggggctc
    32641 cagagcccca cgatccgcag ggcatccagg ccgtcttcgg cggtatccac cgtgcacccc
    32701 gcttttttca gggcgtcggc gatgatggcc cgattggtag cgttgtcatc caccaccagc
    32761 acccgggggg gggtgaggcg cttccccacg tccttgcgcc tcaaccccag caccaggggc
    32821 acgcggatct tccacagaag acctccggcg ctggcgctct cctgccagga gagggaaaac
    32881 atgtccagga ggtatcgcac ctcccgggag agcaccccgg gggggcccgt aaaagacccc
    32941 gcttccaaca cgcactccat ctcggccccc gaagggcgaa ggatgcaggc caccccctgg
    33001 gctccccgct ctccgtaggc gtattggatc atgagggcca gaagaagcac cgccccctgg
    33061 gaaaggcgcc cccgaggggg gaaacgccct gcggagaagg cccattcccg cttttccagg
    33121 aaggcccagg cctcccccag ggactgaatc cgccgggata cgtcgtgaca ggccagaaag
    33181 accgacccca gatgggccat gtcgggcagg gccatgacat cgatgccccc ttggagcagg
    33241 gccaaggcgg cggtgacacg gtttctcccg tcctccgaag gggggcaggt ccccccatcc
    33301 agagccagtt ccagggtcca cagcggagtc cgaaggagat ggccccccaa gcggatttta
    33361 tcggccatca tgcggccgta gaaggcgtcc agggccatca cggcggcgac ggcgacgccc
    33421 tccaggtccc ggatctttcc cgccgacagc cccccggcgg ggttgcccac gccgaaccac
    33481 tcgtctccgg agatgccgga aaccacggcg tccatcgagg atgccacttc cacgcccccg
    33541 aggatcacgc cgtttttcct ccccagggcc cccggacctt cggggcgccc ggcgtcgagc
    33601 gacgccagct cctcgggaga aaggggattc aggcgccttc ccatggaagt gaaaagccag
    33661 gcggccctcc ggggaggggc gcaacgccac accgccaggg gagcgagaaa tcgatcgcac
    33721 aaaaactcca aaagttcccg ggcaaaggtg ccctcgtccg gggcgtggcg tagacggcgc
    33781 aacagagaag gggcgagacg ttcttccatg actttacctc cttcaacagg ccgtttcccg
    33841 agggcggccc tccgggggtc cggggcgtcc cacggtgcct gtgggaggct ctcttcggct
    33901 gaaacaaatt gtaacctttt cttatcgccg gtgcaacgcc gcctccgtca gttctgatcc
    33961 gcgaagcgcc gccagaagcg ggccaggcgc tgccccatgc cccgctcctc ccccagcacg
    34021 ccggggaagt agaagcgttc cgttctatcc atgtaggctt ggggcaccca atgccggggg
    34081 tcgtcgtggg ggtagcggta ccccgatccc gagggacgca ggtgctccgg caccggctgg
    34141 aggttgcccc cctccaccgc tcgcacggcg gcgccaatgg ccccataggc gctattgctc
    34201 ttgggagccg ccgccaagta gagcgtcgcc tccgccaggg ggatgcgcgc ttcgggccac
    34261 cccgtgaagt ccgccgcttc cgccgccgcc gtcgccacca ccaaagcctg gggatcggcc
    34321 aaccccacgt cctcggcggc ggaaatcacc aggcgccggg ccacgaaacg cacgtcttcc
    34381 cccgaggcca ccaggcgggc cagccagtag atcgccgcgt cgggatcgga accccggatg
    34441 ctcttgatga gggcggaaat gatggcgtag tggtcatccc ccgcccggtc gaagcgcagg
    34501 gccccccggg ggagcagagc cgccccggcc tcgggggtca agaccttacc tcctcccacc
    34561 gccacggaag acacggcgac ctccaggcgt agcagggcct ggcgggcatc cccccccgcc
    34621 ccccgggcca gggcccccag gacctcgtcc ggcacggcca cctccagggt ccccagcccc
    34681 cgctcccggt catccagcgc ccggcgcagg atctcccgca ggtcggcttc gtccagaggt
    34741 ttcaggtcga agaccaccat gcgggacagg agggtcttgt tgatctcgaa atagggattc
    34801 tccgtggtgg tgcccaccag gatgaggtct cccccctcca ccgccgggag cagggcgttt
    34861 tgttggatct tgttgaagtg atagatctca tccacgaagg ccaccgctcc ccgccctccg
    34921 tcggcctgtt ttcggcggcg ggcccgctcc accagctctc tcaggtctcc cacccgggcg
    34981 ctcaccgcgt tgatctcgaa gagccctcgc cgggtgccct ccgcgaggag gcgcaccagg
    35041 gtggtcttgc ctacccctgg ggggccataa aggatgcagc tgggcaggat tccctggtcc
    35101 acgaggcggc gcaggggcgc cccggggccc accaggtgac gctgccccac gtactcggcc
    35161 agcgtccggg ggcgcatgcg atccgccagg gggcgctcgc ccccccgatc cccctctccc
    35221 ccggagggga agagctcccc ggcgtcgctc acagccccaa cgcctccttt aggatcgggt
    35281 aaagtccttc ggggttttcc ggtagcgcca gcgcccgggg cgtcggcgcc cccaccgtca
    35341 ccagaggata gcgtccggcg cagacccagc gcaccacctc gtccagcgga catcccatga
    35401 gctccggagg caagcctcgc caggcccgcc actgccccgg cgacagggtc ttttcggggg
    35461 agacccccag ggtacccacc cccagggcac agctttgctc catcacggcc cccagaaggg
    35521 gtgattcttc gggaaggcgt ccctccaggg cccaggcccc ctggagccac cgggggtcca
    35581 aggaggcgta ctcccaagga acaaggggtt cgggcgcccg cagggactcc accagatcct
    35641 tcacccgggg aagggccaca tcggcccagg agctccagct ctcggggccc cgctccacca
    35701 gcacggccct catgcccgcc cgccgagccc ccaggatgtc gtagagaaaa tcccccacca
    35761 ccacacagtc cttcgccccc acgccgagcc tacgggccgc ccggcagagg gcctcggggt
    35821 ggggtttcac cggcttttcc tcccgggaaa acaccagggg gggcagggtc aaccccatga
    35881 cctccgccgc ccggaagatg gattcccggc agttccggga gaccacggcc cagggccttc
    35941 cccggtcctc gatccaagcc agcagatcgc gggctccggg caccacctcc gcctggtccg
    36001 cccccgccat ctccagggcc ctcatggccg actccagctc ctcgcgccgg tcggggggca
    36061 acgagggcaa cacctccagg atcatgaccc ggcgcccctg gaaaaatttc tcgtaaatgg
    36121 gcacaaagga cagacgggta tccgccagca cgccatccca atcgaggatc catcccccgc
    36181 aaaaagcggg gtgccagaag ggggcgcgcc ccgagctttc catctcttcc acccctttct
    36241 gtttccctaa gggagccttt gccgcgaagc ctccgaagac ctggcttcgc ccccctcttc
    36301 gattctacca agaccctacc aaagcacaga ggggagaggg cttctgccct tcccccctct
    36361 gtggccgtcg caaagccccg cagtgccgtg actcgaaggc cttgacccga tcctaacact
    36421 tttgggagcc tgcctcgctt gagctttgtc ggcgggtctt tccgcctccg tcgccagcga
    36481 ggtgccagcc cccccttaga ccgagtgttg gctcaaggca cttctcgatg acacgcacac
    36541 cgcaggatac cttgttcccc atcatggtag cacgaaagat cttttcagac aacctccacc
    36601 gccggtctcg acaaaagggc aaaaaaatcc gccgccgatg cgccaccatg ggccgcccct
    36661 ggcgatccca cgcagcgaaa aggccccgcc cgacctccgg caggcccccc gagaaaaagt
    36721 cgccccccta ttttcccccc acgaaagatc ccagggtcag gtctacggcg gacgccgtgc
    36781 catggtgttc atagctccac cgggtgatcc ccaccccggg gacgagctcc atggtggtcg
    36841 tgtccgggtt ggtccgatag accacgacga aggccttacc cgagggcgat gtcccgggga
    36901 tcgacggcac atcgctccgc tcttcttcca cgtaccactg gtacatcgtg tcatcccggg
    36961 gctcgtcgcc cccccaggct ttgtcctttg ccaagggcag ggccaccagc agatccccct
    37021 cggttccggt ggagggatcc tccgccagag acttggcaaa ggcccgggcc tccagctcgc
    37081 tggcctgggg gaagagctcc acatgacttg cccgttccac cagcaccgca tatccgggct
    37141 cctgccccgg ggtgtagccg ctggtctcgg aaaccatgcc gcgcatcacg gcaacggtgc
    37201 ttccggagga cgaaaagact tcgagggcct ccatgtccca ggaaaggggg gccaccttgg
    37261 gcccttccgc cccctcctcg gtccactggg ccgtcccttc gtagtgccac cgcgcccccg
    37321 aagtcagagg gaaaacgccc tcctccccaa aggccatccc cgcccaaaag atcccccatg
    37381 ccgccaccgc cgcagcgccc cagcgtttcc aacccatcct ggcccctcct ccatttcggt
    37441 cgtcttcacc catgacatcg ttttcccccg cctccttagc gtcggaaggg acctccatgg
    37501 tacgccccgg gccccacctc tggcaacctc cccccagagc cttttcgccg cccccgccgt
    37561 cggatggggg gcgggccaaa aactaaggac ctaaggcccc gccccaagtc ttcgagcccc
    37621 agcggagccc ctctccgaga cggggaagga ggagccgtcg ccccgagggg cctttgctag
    37681 aaggtctccc acccgcgctt gagagggcgg ggttcgggcc ggggcccggg aacggggcgg
    37741 atctccgcct cgctccactc cccctttagg aggctcaggg cgcttcgaat ttcctccggc
    37801 agcacgcccc cctgctccat ctcaagttcc agcaccaccc gacgggaggg cccctcccgc
    37861 cccacccgaa agtcccggat gtcccgcacc ccccacagga tttccgccac gtcgtagagc
    37921 cccggggcct ccgccgtgcc gagacgcccc cgcacctcca ggcggcgcag gggggagccg
    37981 caggggcacg ggccggggat caggcgcccc tcgtcaccgg tgcggtagcg gatcagggga
    38041 aaggtggagc gggtcagggt ggtgatcacc acctcgcccc agcttccgga tggcacgggg
    38101 tgcccccccg gggacaccac ctccgtgaga acctcccctt cccgcaggtg ccccccctcg
    38161 tgggcgccgc actccagggc catgccccac cccacctcgg tgaggccgta gtggtcgaag
    38221 atctccccgg gccaaaggcg cgcgatccga cggcggagcg ccggggggaa catgtccccg
    38281 ctcgccaaaa ccgcctcggg gacgtggcgc gcgacgcggg ccacccgcag gagcgcttcg
    38341 ggcatgccca ccaccacccg ggggttgaag gcgtcgatcc gggcggcggt ttttcgggga
    38401 tcttccccca cgccccccac ctccaccgca catccgagct cccccagggc ttcccgcaac
    38461 agggccccca cgctcccggg gcgatctccc cggaagagca ccgtcacccg gcttcccggg
    38521 ggcgtaaagg cggtgagccc cacgcggaaa aactcccggg tggcggcgag gtcggcgtag
    38581 gtgaaacaaa gcctcttggg gggaccggtg ctaccggagg tggcgagggt cacgatgcgg
    38641 gccacctcgc tctgggacac ggtgagaaaa tccccctccc gtcccgccag atcggaagga
    38701 taggtgaagg ggagctcctc cagaggagcc ccctctcttc cccggagccg ggaggcgtaa
    38761 aggggcgaat cctgggccgc ccgggccacc agggcccgga gccgctcctt ctggaaggcc
    38821 tccagggcgg cgcgccccgc caggggggca ccttccccca gacatcgggc gcaccagaga
    38881 tccaggggcg tggagcgaag gctcatccgg ccccccggcg gtacagcggg gtgccgtccg
    38941 ccgccgtgag attccaggca caaaagggca ccagacgtcc gtcgggggcc gcagcatgga
    39001 tgcagcactc ccggaggcgc cggaggtcca ggttccaggc atcctgaaag gccatggcgc
    39061 tgacgctgaa aagaacccgc ccttcctggc ggagaaagcg gtccagggcg tctccagcgc
    39121 cgtcgtcatc ccttttttcc agggaagctc cccaccgtcg ccggacgctc tccaccgccc
    39181 gggatcttcc ctcttcggcg gattgggatc ggcaggcaca gggcgttcct cccaggggga
    39241 cgagacggcc ggggccctcc acccgatacg ttcctcggca ggagcagcgc tcgtgctcgc
    39301 accccgcggg gtgaaagtgc tcccggcgca ccatccctcc ggtctgttct tccagcaacg
    39361 ccaagacctc cggcagggtt atccgccggt ggtcctgggg ctccagggaa tagcggccga
    39421 aaaagctcat gggctgcacg tggacccccc gcacggtggg aaagtgcccc agggcgaagc
    39481 gcaccagatc tcccagctcg ccgtcgttca ccccccgcac cagggtgggg acgaggacca
    39541 cgccgagctc cgcccgggcg caggcctcca cggcccgctc ccggggctcc cggagatctt
    39601 tgtcccgcag cctacgggac gaccgctccg aaaggccgtc gaactgcaaa aagacccacc
    39661 ccagccccgc atcccgcagg cgaggcgcca gagccggatc ttccgccagg gccaggccgt
    39721 tggtgttgag ctgcaccgcc ccaaagcccc gctttcgagc cccctccacc agcaggggaa
    39781 gatcggggcg cagggtgggc tctcctccgg agagctggag caccgcctct cccacctggg
    39841 gacgcatttt ttccatccgc tccagcaaca gctccagcgg aggatcgggg tcttcggagc
    39901 ccgcatcggc gaagcacacc gggcaccgga ggagacaccg cgacgtcacg tcaagcacca
    39961 cggcacaggt tttttgtgcg tgaaaggagc agagaccgca cgccccgggg cacgcggggt
    40021 ttcggccgag ctccggtgcc ggaggggcct tgggcccccg ccaggccttc caggactcca
    40081 cgccgcgcca gaccacgcag gaaaaatccc cgtgctccgg acagcttttt ttcataaaaa
    40141 cctcgtcccc ctcgggcacc aaaagggccc ccacggggcg gaggcacacc gggcaaacgc
    40201 tctcggtctc gtcggggagc ctcacgacga cgccccgaag aggtcaacgc cgtggctctg
    40261 gaggatctcc aacgtccgcg tcagggtgtg ttgaaccagg tggagacagc gcagggggtc
    40321 gggctcccgc accgtttccc ccagcagatc gccacaccgc accccccccg tgccccccgt
    40381 ctgggtctct cgaaaccact gattcagctc ttccgccatg aggcgcaacc ggggatcccg
    40441 gagatcttcc gcctccccct tgccggcgta gagccccagc agacaggcgg cccccgtgag
    40501 ggcaccgcag gtattccccc gggagaaggc cccctgacac aggccgttcg ccgccgccac
    40561 gagatttcgg tcttcctccc cccggtacgc gagccccgcg agcaccatga tctgggcgca
    40621 gcagaagccc tcccgggccc aggccacgat ccattcccgg agatcgttct ccccttccaa
    40681 cgtcatcccc tccccgcatg ttggcctgac cccgggcccg gagaactcgg tttcccgctc
    40741 acccaaaggg cgtatccccg ccgaaaggga gcggtccagg cctcgcagga aagaattccc
    40801 gcccaaacca ggcgggccgc cagctcgcgc acggcgcggg agtgatcctc ccggtgcctg
    40861 agaagaaacc ccgccccggc aagggatcgc tccatctctt ccccccgccg ggcccctccg
    40921 acgcaccccg cacaagggcc ccctccgggg ggaacgtttt cgggaagaaa gagatctccc
    40981 acccccagca cccctccggg acggagcacc cgaaagaact ctcccagaat gctccggggg
    41041 tcggcgctct gagagagcac acactcgcac accaccccgt cgaagcttcc gtcgtcaaag
    41101 ggcagggcct ccagagtccc ctccaccacc gggccccgcc gggcggcgca ccgcagccgc
    41161 tccggggacc ggtccacccc caccgcctgc caccctcccc gccccaaaag ctccagcctt
    41221 cccccccggc cggcgtctcc gccgcagacg cctatcaccg gcagg
//
LOCUS       DAOOER010000013        36637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_1268_length_36637_cov_8.373626, whole genome shotgun sequence.
ACCESSION   DAOOER010000013 DAOOER010000000
VERSION     DAOOER010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 36637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36637
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1268_length_36637_cov_8.373626"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PL162_03135"
     CDS             complement(<1..402)
                     /locus_tag="PL162_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HQF91059.1"
                     /translation="MLPEDVKMTPMLRQYTEWKERYPDCLLFFRMGDFYEMFFDDARV
                     AAEVLDIALTARDPERRIPMAGVPGHAVDSYLGRLVRGGHRVAICEQIGDPQGGGSLV
                     ERRVVRVVTPGTYVPDEGGSEGRLAAVSGERG"
     gene            complement(402..824)
                     /gene="ruvX"
                     /locus_tag="PL162_03140"
     CDS             complement(402..824)
                     /gene="ruvX"
                     /locus_tag="PL162_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HQF91060.1"
                     /translation="MMGRIIALDMGSVRIGVAVSDPLGVFAQGIAVLPAEGPWMRRLE
                     ELMTSYAADRILVGFPRRTSGEEGPEARRMMELVALLRERFPGVTVETEDERFTTVMA
                     HRAMREGDASRGDRRRKVDGVAAALLLQGYLDRRRNAS"
     gene            complement(821..>2167)
                     /locus_tag="PL162_03145"
     CDS             complement(821..>2167)
                     /locus_tag="PL162_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HQF91061.1"
                     /translation="ASSKQRSSSLRGNVYTEMLNEFGKTLFLGYDQESAKTSVLALLK
                     DGERVAEVNEGDEVEIVLAETPFYAERGGQLGDTGEIHHPGASVIVMNTTYGAGELVI
                     HRGKVDSGALAVGGGVVAAVDGNRRRGIRRHHSATHLIHEALVRRLGAHVRQAGSLVS
                     DEFLRFDFTHFEPLRTDDLEAIELFVNEEVMRNTPVTVEITTLDKARDKGAKALFDEK
                     YGDEVRLVAMPGTCAELCGGTHVGATGEIGAVKILREEGIGAGVRRLTAVAGISALRH
                     HQDLSRSVHHLRSMLNVEVEELPLRVEGLQEEIKHLSRALGEARLRGFIAAVPSLMEK
                     AVTVGALSVITGAFDEAEADLLLQVGDRIKRSLSGVVVVLAGRFADRVQFVAMADAEA
                     QAQGAHAGRIVKETVARTGGGGGGKADLAQAGGKQVERTSEALEAVPGIVMALLGA"
     assembly_gap    2168..2267
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2268..3184)
                     /locus_tag="PL162_03150"
     CDS             complement(<2268..3184)
                     /locus_tag="PL162_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HQF91062.1"
                     /translation="MIYRSGGELRELFLSYFEGKGSRRYPSFSLRPEDPTILFTIAGM
                     VPFKPFFLGIRTPEVKRATTSQKCVRTNDIDNVGRTARHHTFFEMLGNFSFGDYFKKD
                     AIPWAWEFLTDRIGLDPDHLYATVYLDDDEAADLWHQAVGLPQSRISRLGADDNFWAV
                     GPVGPCGPCSEILYDQGPDFSCGRPDCAPGCSCDRYLEIWNLVFMQYNRNEAGELIPL
                     PKKNIDTGMGLERLSSVVQRVRTDFETDLFSPLIQAAATVTGVSYGVSPRETMALRVI
                     ADHLRAVSFMIADGILPANEGRGYVLRRLL"
     gene            complement(3206..4501)
                     /locus_tag="PL162_03155"
     CDS             complement(3206..4501)
                     /locus_tag="PL162_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQF91063.1"
                     /translation="MLFRNVCMVDADLPSGVYGDVLVAEGRIAAILPPGQGRDDEVVG
                     GGGRRALIPGLVNAHTHAAMTLLRGLGEERPLMEWLQQAVWPVEARLKEDHVYWGTML
                     AIAEMASQGITCFGDMYFFMDEVARGALESGLRCGLSRGIVWGDSPGENDRRLKEQEE
                     LFRAWHGCQDRISVQYGPHAPYTVPPERLKQIAELAKDCGAGVHIHWLETTWELDYIR
                     NTLGKDPVDLLDEVGLLEAPRLLLAHGVHFPVDRLKDLARPNLTVAHNPGSNMKLGSG
                     VAPVPAMLAQGVHLALGSDGAASNNRLDVWGEMRLAALLHKGLSRDPVAVPATSVLRM
                     ATVEGARALGFDDVGLLREGWQADLVLVDLDLPHYVGWTPETLCPYLVYAGSSADVRG
                     TMVQGKWIYRENNFVTMDIAQVKRESSRCRRELVKDQKD"
     gene            complement(4815..6059)
                     /locus_tag="PL162_03160"
     CDS             complement(4815..6059)
                     /locus_tag="PL162_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HQF91064.1"
                     /translation="MTASRIAHPEQAPRGQEKIHWAWDAMPVLRYLAQKHGPSKPLAG
                     RRVTVCLHLEAKTACLLKTLKLLGAEVAAAGSNPLSTQDDVCAALVEEGIAVYSHRGM
                     DPETYCRYLRDAISFDPDIIIDDGGDLVSLLHEERPDLLPRIRGGCEETTTGVKRLRA
                     MELEGVLKFPMLAVNDAFSKYLFDNRYGTGQSVWDAVMRTTNMVVAGKTVVVVGYGWC
                     GRGVAARAQGLGASVVVVEADPHRALEALMDGHGVASMIDAARKGDVFITVTGNIHVL
                     RGEHFDVMKDGALLANAGHFDVEISKPDLLARSEHVESYRPNVTTYGLRDGRRLHLLA
                     EGRLVNLAAGDGHPIEIMDMSFALQLLSVLHLCHTELPPGVHRVPDDLDRQVALSKLA
                     SLGLAVERLTPEQEAYLSSWKE"
     gene            complement(6204..7253)
                     /gene="mtnA"
                     /locus_tag="PL162_03165"
     CDS             complement(6204..7253)
                     /gene="mtnA"
                     /locus_tag="PL162_03165"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HQF91065.1"
                     /translation="MIVPLVWKKDRLEILDQRRLPGSVEWIRCDDPEAVASAIEALAL
                     RGAPAIGLAAAYGVVLGVKRGEDLDRVVARLARTRPTAVNLFWALGRMRDQWEAMGER
                     MGRQERIQRLEELACRLEREDRAVNRAMGSFGAALLPDEGMVLTHCNAGALATGGHGT
                     ALGVLRSARDQGKRLKIFADETRPVLQGARLTSWELSQEGMDVTVICDGMAAALFSRF
                     PLSAVIVGADRVAANGDVANKIGTYGVAVAARYHGVPFYVAMPRSTVDFYTATGADIP
                     IEIRKDEEVRRWGDGIVVAPGASVWNPAFDVTPGDLVSAFITEVGVLHPPFDQSLKEA
                     RDRPFVPEDDIYWES"
     gene            complement(7250..8491)
                     /gene="xseA"
                     /locus_tag="PL162_03170"
     CDS             complement(7250..8491)
                     /gene="xseA"
                     /locus_tag="PL162_03170"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HQF91066.1"
                     /translation="MEPRSLSVCELGAYLSQRFSEDLLLRHLWVRGELSEIKRHSSGH
                     TYFTLSEGESRLGGVLFRGDAFRQISWPRVGDAVLVEGRIAVYEARGIYQIYALRLIP
                     LGAGAQSRAREELRRLLEREGLLDPRLRRPLPPFPEVVALVTSPTGAAVQDVIKVGGK
                     RFPSCRFFVVPALVQGLGAPASLVRALRRAGHVPGVEAIMLVRGGGAREDLSPFDDEQ
                     VVRAARHSPVPLVTGIGHQTDLSLCDMVADASTPTPSAAVEKLLPDRQEILDRLESLA
                     RRGHMALSRSHDRASLQMDQWTQRGSRAVRSYLKEGEKTLDRLIQRHGAALGSALDGV
                     GKALDLMEERLEALSPWHPLRRGFPFCEREDGTPLVRGEELFPGDRLRLRFMDAVALA
                     QVEAVERAIPPSEAFERGIKP"
     gene            complement(8491..8931)
                     /gene="nusB"
                     /locus_tag="PL162_03175"
     CDS             complement(8491..8931)
                     /gene="nusB"
                     /locus_tag="PL162_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HQF91067.1"
                     /translation="MGQAVLPQRRRRSREIALQLLYALDLRTEETPASLWALQDFDEE
                     DGVEEYARDLLEGTWDHRIEIDQMIRTHVVGWRPERMVAVDRAAVRLALFEGVVARKT
                     PLAVAISEAMELAKQYGTEDSGRFVNGVLGKIVRAQESPSGEEP"
     gene            complement(8981..9496)
                     /locus_tag="PL162_03180"
     CDS             complement(8981..9496)
                     /locus_tag="PL162_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91068.1"
                     /translation="MFLWWQQTDRGNLWIESRGVRQLLEGMLPERCVVRAVDFWKERQ
                     TAAVRLGYANGEGGVSDEALRAVGDDVRAFLAPLGFGVVEVSWTEEGEVEDHSWRRWM
                     GRPETWGVAAGVLVAVGRLGWDGTFGALIAGGGAFGVAWLVLTPRGQDKWKIFCRKAI
                     DLWYGGAPRPR"
     gene            complement(9548..10117)
                     /locus_tag="PL162_03185"
     CDS             complement(9548..10117)
                     /locus_tag="PL162_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HQF91069.1"
                     /translation="MEEKERDLQMREEEILEDQDFTESEEKEESAAPRGGEVRISEDV
                     ILQLATQALRSVQGVRPAGASPLASLGLGRKTSGGMRVSLDDKTPPQILVDAYVAVKY
                     GLRIPDLAWDVQELVKNHLEKFTGYRVKSVNVFVQSIYLNDVEAEAPAATVGDGAAAG
                     TLNSGDDGPTLSISKEGCPSSGARADEGV"
     gene            complement(10196..10768)
                     /gene="efp"
                     /locus_tag="PL162_03190"
     CDS             complement(10196..10768)
                     /gene="efp"
                     /locus_tag="PL162_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HQF91070.1"
                     /translation="MGQIVDTSDFFVGLKVRWQDGIWEILDYQHHKMGRGGATVKTKA
                     KNLLTGSIVETSFRSGERFERIIFDQKPAQFLYREGDLFVFMDLESYDQLSLSPEILG
                     DMASYLTENLEVKIDMHEGRVMEIELPKSVELRVTQTEPGFKGDTVSGGGKPATLETG
                     LSITVPMFINPDEVVVVDTRTGLYLERAKK"
     gene            complement(11003..12610)
                     /locus_tag="PL162_03195"
     CDS             complement(11003..12610)
                     /locus_tag="PL162_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HQF91071.1"
                     /translation="MVGGVEIQQLGDKAVLCHIKGSSLPLPVLQREDPDEVELFFPKL
                     SIPSGTWERAYDDVPLVKKVRMEQQERGMALVFETNHPLRVRQAKGVAPANQLEIFLE
                     VKPATKPLTVGPATPSGVPAVARGGDPLYSNKPVTLELREEDLKQVFFMLGKMLGLNI
                     ICDPSFPSAAVTVSLKDVPMREAFAYLMRMYDVQYAYMGNSLILGKSGQLARALGKES
                     TRVFRVAYAKPEDVVPLLQGLVRVDKVTVDARLREIYVTAGEDILNEVAQTLERIDHP
                     GRQVMIQARIFEVSHGNEDKLQSVIAHVYKHWYVTYGQGGMSIGYLDTNKPPKYDPER
                     QILDGLDPEDLSQGALRLLDAGLNALQTDGQGKNLARPSVVTVDGMKATIDLTTSVPY
                     ISSYKDDNPVYSTATAGPKMDITPRIGRDGLVNLEMTLSTGTLDFSNGDTPATSTRSV
                     TTNVRVRNGEPFVVGGLFQEVTSTQKTSFPVLGNLPLLGEIFRWESKSHKKSEVAMIV
                     IPYILDIPNAPVESDELFAPTAHRRNP"
     gene            complement(12751..13335)
                     /locus_tag="PL162_03200"
     CDS             complement(12751..13335)
                     /locus_tag="PL162_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91072.1"
                     /translation="MSAQETWGEALDALWGRLGSGDTVLSQVMRYGLVLLLLAGAAWQ
                     IKSGHDMMVELEDPVPLFAPPVNTIGEQGRVEELLRVFDGVMTLRTGGKDTVTIMEAT
                     HRQPYRPNVGETAASDLVPVAEALPPLMTVRSIMILEDRRAAVMDIETEGTGIIVTEG
                     MKFDKGHGRILKISKDRVVVVWMRKRMEIVIDPR"
     gene            complement(13335..13859)
                     /locus_tag="PL162_03205"
     CDS             complement(13335..13859)
                     /locus_tag="PL162_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91073.1"
                     /translation="MGTKRIVLGLALLLTVGGGLGGTTVLRWKLQENIDRQRELQRQE
                     VQYRHSSETNAALIQLYKKTLASLEKSQVGFPGDQVAFLSAVEQVLTQEGLEKISMTT
                     AGVSPEGKISVAVQFRGPYYKVMRALAEWRALSSLVRMSTINFNMDRKGDVQANTVLE
                     TLIGLSPRRAGGES"
     gene            complement(13875..14447)
                     /locus_tag="PL162_03210"
     CDS             complement(13875..14447)
                     /locus_tag="PL162_03210"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HQF91074.1"
                     /translation="MTIRFDLRPPQYRQTQKRYAVDRGKILFFLALLLMMLSSGGVAG
                     YQAFRLHALSLSLMDLESSVAALQAQDGKLARTVQDLQQEIAFYEGARDLVKEELTAL
                     ELLGAMEGALPGTVWIERVDVVAGAATLTGLGFSEGDVVIFSNALANAAVVAEVLVPV
                     ISQSGEGLKRTVRFTMTCKLRDLTALITPR"
     gene            complement(14444..15385)
                     /gene="pilM"
                     /locus_tag="PL162_03215"
     CDS             complement(14444..15385)
                     /gene="pilM"
                     /locus_tag="PL162_03215"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HQF91075.1"
                     /translation="MALWRKNTARASLSLVSDGLRYLALDGTTGHFKLTQAKAVTTPH
                     PFLQQGMLPDANRAVPLLEKLRQEMDGSFLKVPVSISLPARDVMLRVVDMPRMGAEEA
                     RDALQWDFDKYFPFPAGEAVYDVCPLEVPGGEDAEKMKVLVAAARLRMVETVMDIVVK
                     GGFSVGGAEPILVGIFRSLLGPMPSFKSGALLVLPEKQSTQIMVAYRSSGLLFRTLLV
                     GFGAGGDREEMVRTIGREAGSTAAFVRNQFRELSVDTVLLGGEFSREPGLDEEIRSIM
                     PDVAVQAVAMWDQWGISNPPSGGEWEGALGLLVGGEP"
     gene            complement(15793..16350)
                     /gene="frr"
                     /locus_tag="PL162_03220"
     CDS             complement(15793..16350)
                     /gene="frr"
                     /locus_tag="PL162_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HQF91076.1"
                     /translation="MPAQELKQIRDRMEKTVEHLRVECLGVRTGRAHPALVEDIKVDY
                     YGAQTPIKQLGTVQVPEPRQILISPWEKAALKAIEKALLASPLGITPQNDGENIRLRL
                     PELTRERRVDLTKLVRKYAEEAKVAVRNLRRDGNETFKKMEKDAKITEDALHKYQKEL
                     QDSTDAVIKQIDSVLEEKEKEIMNE"
     gene            complement(16332..17078)
                     /gene="pyrH"
                     /locus_tag="PL162_03225"
     CDS             complement(16332..17078)
                     /gene="pyrH"
                     /locus_tag="PL162_03225"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HQF91077.1"
                     /translation="MGMWRRVLLKLSGEVLAGGERFGLDFPTVDALAEEVADVARSGV
                     EIAMVVGGGNIFRGAQASPKGIERAQADNMGMLATVINALAFQDSLERAGMPCRVQTA
                     IEMREVAEPFIRRRAVRHLEKGRVVIFAAGTGSPYFSTDTAAALRAAEIHADCLLKAT
                     KVDGIYERDPRIHPDAAFFSTITYAEALRRRIGVMDAAAFSLCMENRIPTVVFNILHC
                     GNLRRLLVENECLGTRVYAGEEETTCLPKS"
     gene            complement(17159..17752)
                     /gene="tsf"
                     /locus_tag="PL162_03230"
     CDS             complement(17159..17752)
                     /gene="tsf"
                     /locus_tag="PL162_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HQF91078.1"
                     /translation="MAGEMEMVKELRARTGAGVLDCKKALEECHNDVEKAVDYLREKG
                     LAKAAKKAGREASQGMVFSYIHTNGKIGTLVELNCETDFVARTDEFRQLGHEIAMHIA
                     AANPLYLSPEEVPAGEVEREREIYRKQAEEEGKPPQIQDKIADGRVKKFYDESCLLEQ
                     AYIRDTDRKIKDLVVDQIAKMGENIVVRRFSRYSIGQ"
     gene            complement(17759..18649)
                     /gene="rpsB"
                     /locus_tag="PL162_03235"
     CDS             complement(17759..18649)
                     /gene="rpsB"
                     /locus_tag="PL162_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HQF91079.1"
                     /translation="MAIVSMKQLLECGVHFGHQTRRWNPKMKPFIFTERNGVYIIDLQ
                     KTVKGLEKAYDFLREVSSGGGSVLFVGTKRQAQETVRDEALRSGGYYVNQRWLGGMLT
                     NFQTIRRRVLRMVELQSLQESGTLDLYPKKEALELRKELSKLEKYLVGIRDMKDVPDA
                     LFVVDPRREDIAVMEARKLGIPVVAIVDTNCDPEVIDYPIPGNDDAIRAIKLMVGAMA
                     DAVIEGNQGSDAPLTAKEKAATEEVEGVSVPETPGGIAPLAETPEAIEVREKLHDAYG
                     DEGETLAVKGLEERKGWKEA"
     gene            18915..19241
                     /locus_tag="PL162_03240"
     CDS             18915..19241
                     /locus_tag="PL162_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HQF91080.1"
                     /translation="MERTSRVQEIDSLTPEKKAMINRLRRVEGQLRGIQRMIEENSPC
                     GPILVQLSAARRAMHQACIEVLKGHLKGCLTRGQAPDPKELEQLIGALLELAPAGDQN
                     LSEKHE"
     gene            complement(19445..19544)
                     /gene="ffs"
                     /locus_tag="PL162_03245"
     ncRNA           complement(19445..19544)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PL162_03245"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(19570..19663)
                     /locus_tag="PL162_03250"
     tRNA            complement(19570..19663)
                     /locus_tag="PL162_03250"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19627..19629),aa:Ser,seq:gga)
     gene            complement(19705..19797)
                     /locus_tag="PL162_03255"
     tRNA            complement(19705..19797)
                     /locus_tag="PL162_03255"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19761..19763),aa:Ser,seq:cga)
     gene            complement(19842..20360)
                     /gene="tadA"
                     /locus_tag="PL162_03260"
     CDS             complement(19842..20360)
                     /gene="tadA"
                     /locus_tag="PL162_03260"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HQF91081.1"
                     /translation="MSSFPSVELKDEEGMALALEEARRALEEGEPPVGAVVFYGGKLL
                     SRGRNARETSQDPTAHAEILALRRAADRRQTWRLDGCTVYVTLEPCVMCAGALIASRV
                     DRVVFGAADPRFGAWGSLYDLGKDGRLPHRCLVRRGLRSGECQALIDQFFSTVRARSR
                     RPRGLTEAMERV"
     gene            complement(20353..21168)
                     /gene="speD"
                     /locus_tag="PL162_03265"
     CDS             complement(20353..21168)
                     /gene="speD"
                     /locus_tag="PL162_03265"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HQF91082.1"
                     /translation="MKLYGFNNLTKTLSFNMYDICYAKTPQHRRDYIAYIDEEYNAER
                     LTSILMEVSHIVGAQVLNIASQDYDPQGASVTMLISEEKMEETEKGDHDQEGGEDQEG
                     ESPGPLPEAVVAHLDKSHITVHTYPESHPHKGVSTFRADVDVSTCGRISPLKALNYLI
                     HAFDPDIAIIDYRVRGFTRAVDGEKIFIDHKINSIQNYISSETRDRYQMIDVNVYQEN
                     IFHTKMALREFDLDNYLFEGGKKDLLPREKKQIKQALKREMYEIFVGRNIPHV"
     gene            complement(21249..21806)
                     /locus_tag="PL162_03270"
     CDS             complement(21249..21806)
                     /locus_tag="PL162_03270"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HQF91083.1"
                     /translation="MVRLLVFLWALGASGEAGALERVCPTEQRVDYVGMAVKVGDKLP
                     NSSLFLPQEQRAARIRDLAPGTLTLLVFWSATCPECLDEIKALPELRARFSEEDLSIL
                     SIAPAYDDGQMRAKYGDASWDAIKAGLGATVACIDDYGSATLEYKVDEVPYMVLIDKD
                     NVCKTWVLGKTAVEDLVRVIERQMP"
     gene            complement(21864..22499)
                     /locus_tag="PL162_03275"
     CDS             complement(21864..22499)
                     /locus_tag="PL162_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91084.1"
                     /translation="MTTTVQDPHPSEGLPASEELSGIEGGKIWQERLEQALRTQGFSD
                     LSSGEDLSFALMNLISIEEHLACSFARTGDPFFVEALPDIRNLRRDLMKQVVREGYGE
                     GWCISKHLLATAMRLLEVANKRQAEGQEEMSHRLIEEAFTLWSMFWAFTGPGEKGASL
                     PPRSREGRIPLPQALPGGVGVGLRSDVVPPLNSKKGLSRLVQAFIDCCRDG"
     gene            complement(22530..23048)
                     /locus_tag="PL162_03280"
     CDS             complement(22530..23048)
                     /locus_tag="PL162_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91085.1"
                     /translation="MRRIVPWSAGTAVAAGALGAKAAEASCPLCFVTAGVGLLLAKYF
                     GLRLEVVGALTGGLSAVAALWMHYALRRYRFPYRPVVFTALMVFFNNMIMIPGNGVTW
                     QRLLFLLIFSPLAAWAWFGALVLFVAILLSKAQKRAFGRTLFPFQTVAVSAVAMALVC
                     AVPALVGYWRGV"
     gene            complement(23241..23594)
                     /locus_tag="PL162_03285"
     CDS             complement(23241..23594)
                     /locus_tag="PL162_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91086.1"
                     /translation="MKKLACGVVLVALLAWAGLAWAGTQDFTLVNDTGVVINAVYCSP
                     SSENNWQEDILGVDTLASGESVTVKFSGFDHCQWDLRIEDTDGNALEWTGLNLCDISR
                     VTLHWDGSNATADLE"
     gene            complement(23841..25232)
                     /gene="rlmD"
                     /locus_tag="PL162_03290"
     CDS             complement(23841..25232)
                     /gene="rlmD"
                     /locus_tag="PL162_03290"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HQF91087.1"
                     /translation="MGKPLARGEAAEVTISGINSRGEGIARTDEGFVLFVPQGLPGER
                     VRVAVVEAKRQYALGHLQEILGPHPRRVSPPCPWFGACGGCQIQHADEILQTELKGAL
                     VQDALTRLGGGVPETGFEACRPSSVLWGYRNKAAFPAVFHPSGGLRLGFYREGSHRIV
                     PLRQCPVVDPRLEGLFISLRPLLEKGALTGYDEKKHSGDLRHLVLRGGIRTDHVACVL
                     VVRAPLSSPQERWLREKILPVVRSAAKRYTLAVHIHGAPGNTIWGGTTEIVGGESFLS
                     ERLGDFSYTYDVTSFFQVNPTQAEGLFSHVADLVKAANPSRTLELYAGVGSLTTFLAR
                     LGGEVTAVESWVASVESSRHNLGRAGFRQVRTVCADVKAYLADPGERPDVVVMDPPRT
                     GCEEEVLHRLGALASKTLVYVSCNPATLARDVGVLRQEGYKLSRVTPFDLFPQTAHVE
                     SVALLHREASPSP"
     gene            25706..26155
                     /gene="alaE"
                     /locus_tag="PL162_03295"
     CDS             25706..26155
                     /gene="alaE"
                     /locus_tag="PL162_03295"
                     /inference="COORDINATES: protein motif:HMM:NF018333.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034639 - L-amino acid efflux
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HQF91088.1"
                     /translation="MATDEGESSRHRLSLVLADIVAMIIFSTALCMVIEIFIAGLSFW
                     QSVSARVAAIPVNLVTGRPYGIFRDKLFQILHIDRVNSWKLILGDTLAFVAFQVPLYV
                     VVLLLAEASWRQIAISSASITLIFSLAGRPYGMFLDFCRRIVSGFGS"
     gene            complement(26275..26820)
                     /locus_tag="PL162_03300"
     CDS             complement(26275..26820)
                     /locus_tag="PL162_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HQF91089.1"
                     /translation="MSDRYEIRLAGSGGQGVILAAVILGEAAVLHEGLAAVQSQSYGP
                     EARGGSSKSEVVISKGSIDYPKATALDLQVILTQKSCDEYVKDSKPGSVVIIDDFFIE
                     EIPDVDADVFHLPIVRTAREKIGKEIVVNMVALGAAAGALEKKGIMKPESIRKAILAR
                     VPKGTEELNGRAFDEGYALVK"
     gene            complement(26825..27640)
                     /locus_tag="PL162_03305"
     CDS             complement(26825..27640)
                     /locus_tag="PL162_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HQF91090.1"
                     /translation="MPREQVSKWLRTRFFPHIWCPGCGHGVILHALLRALVDLEKKPE
                     ETVIASGIGCSSRMPGYIDACTVHTTHGRSLAYATGIKMAKPELTVIDVMGDGDCSAI
                     GGNHFIHACRRNIGITAIIMNNNIYGMTGGQASPTTPQGARATTAPYGAIEPPFDICK
                     LAEGAGASYVARATIAQPVMAEQYIKKAIQHKGFSVVEVMTHCHTQFGRKNKRSQPVD
                     NLVFFKDASVPKAKADAMSPEDLKGKIVIGEFVNRDIPEYTDEYRKLCERVRG"
     gene            complement(27643..28776)
                     /locus_tag="PL162_03310"
     CDS             complement(27643..28776)
                     /locus_tag="PL162_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HQF91091.1"
                     /translation="MPQIEFWQGNMALAQGAIAAGCRFFGGYPITPSTEIAEVCAEEL
                     PKLGGKFIQMEDEIGGIAAAIGASIAGYKAMTGTSGPGFSLKQENLGLAYMAEIPLVV
                     VDVMRGGPSTGLPTMVSQQDVMQARWGSHGDHGTIAYAPSSVQECYELAIDAFNMAER
                     FRQPVLIMSDEVVGHMREKVVIPEPGSYMLVDRKRPHATPDAFVPYKADEADDIPPMA
                     SFGDGYRWHVTGLTHNDWGFPTNNHGDIEKKMLRLMRKIDRFRDDIVKYETEAVEDAK
                     VLVVAYGSPARSALRAVRQAREKGIKAGFFRPITLWPFPDKEVGALAAKAHSVLVPEL
                     NCGQMVLEVERAVAGRARVLGQSLVNGELFKPSAILAKIEEVA"
     gene            complement(28763..28978)
                     /locus_tag="PL162_03315"
     CDS             complement(28763..28978)
                     /locus_tag="PL162_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQF91092.1"
                     /translation="MSKVFDVLINKGWCKGCGLCIAVCPKKVLELNDRAKCEAPRVAE
                     CIGCRQCDNICPDLAITVKEREPHASN"
     gene            complement(29314..30465)
                     /locus_tag="PL162_03320"
     CDS             complement(29314..30465)
                     /locus_tag="PL162_03320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQF91093.1"
                     /translation="MSWTHTADYVVIGGGIQGVAIAYNLAKMGARNVVVLEKDTVGNG
                     STGRCGAGIRAQWGTEMNCRLGIASLEIFEQLSDELGMDVGLNQCGYLMVAYKDSEFA
                     TLKHSMALQNSMGIKTRVVSLKEACEICPGLAAEDAVGFTFHDRDGHADPFLTTFAYQ
                     EAARRLGVVFHKFTECTGVKVEGGKVVGVSTTKGDIATPVVINAAGPYAQFIAKMAGV
                     ELPNYSERHEIVITEPVEFGVCPPMLMSFTGNFYIQQRPHGSIIGGCSPEGHPEDFGN
                     GHTWAFAELMAQKITKVLPRAKGIRLVRQWSGLYEMTPDRQPILGEADEVKGFFLSTG
                     FSGHGFMLAPETGVLMAELILKGETHLPIGMLHYRRFASGELINEPAVV"
     gene            complement(30465..30725)
                     /locus_tag="PL162_03325"
     CDS             complement(30465..30725)
                     /locus_tag="PL162_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HQF91094.1"
                     /translation="MVICRCEEVTLGEIREWIHKGYDTFDELKRVLRVGMGPCQGRGC
                     REIILREVSKITGTPYALIPQGTIRPPVKPVKLGALAKGGDK"
     gene            complement(31011..31256)
                     /locus_tag="PL162_03330"
                     /pseudo
     CDS             complement(31011..31256)
                     /locus_tag="PL162_03330"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
     gene            complement(31259..32365)
                     /locus_tag="PL162_03335"
     CDS             complement(31259..32365)
                     /locus_tag="PL162_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQF91095.1"
                     /translation="MRETEILVIGGGPAGLSAALEAASLGAEVTLLDSDLKLGGQLIK
                     QTHKFFGSKEEHAGTRGLLIADLLFRELEDYKSQGKIKVLSNCTATGYYKEDGVVSYV
                     VGEDEYVRIRPRKIVMATGAQERLIPFHNNDLPGVYGAGAIQTLMNVYGVTPGQRVLM
                     VGAGNIGLIVSYQLMQAGVEIVAILEALSKIGGYWVHAAKVRRMGVPILLNHTIKEAL
                     GTDVVTGAVIQEVDSKFQPVGDPQRVDCDVICLAVGLTPTCEMLWQAGCEMKFVPQLC
                     GYVAKRDSLMRTSHPDIWVAGDAAGIEEASSAMVEGRVAGLAAAAALNYGTPEVLKER
                     STMYHKRLSDLRAGEVGEKIREGLCQVCCECWGA"
     gene            complement(32381..32701)
                     /locus_tag="PL162_03340"
     CDS             complement(32381..32701)
                     /locus_tag="PL162_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HQF91096.1"
                     /translation="MNLIEKHPILEFQRGRKVQFFFDGQEMEGFEGEPIAAALHANGV
                     RIYRVTPEMKRPRGFFCAIGKCSSCFMVVDGVPNVRTCVTALTEGMRVETQTGKGLVP
                     PVGA"
     gene            complement(32910..33560)
                     /gene="tsaB"
                     /locus_tag="PL162_03345"
     CDS             complement(32910..33560)
                     /gene="tsaB"
                     /locus_tag="PL162_03345"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HQF91097.1"
                     /translation="MGVILGIDCCLRKTSVGCVRNGAVLGDIHLDLDRRQGALLPVVQ
                     EGLLRALELHLADLDAIALTVGPGYFTGVRVGLAYGTALAEALGLPVVPVGTLEALAS
                     SAVGATHTVVPMIWARHGASYVGAYDLSEGEELLSPQMVEDIELNNVLGLLPSPQIVV
                     GEGIDRWAPWTDSAGVRRVETRISAAIVALLGEKRLGDAMSPQSVRASYLRKHDVN"
     gene            33868..35490
                     /locus_tag="PL162_03350"
     CDS             33868..35490
                     /locus_tag="PL162_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HQF91098.1"
                     /translation="MDVALQLLGGVGLFLYGIKLMGDALQSLAGDRMRRLVGSLTRTP
                     VRGVLIGTFVTMLIQSSSATTVMTVSFVHVGLMTLQQAVGVIMGANVGTTMTAQIIAF
                     KLNAYALPLIGIGMFLALFGKSKKQRYIGNGIVGFGLLFLGMKTMEDSMSFLRGRQDL
                     FLSFSSNPMLGVLAGTVVTMVVQSSAATIGLTIAMAAQGLFGLDVALPIVLGDNIGTT
                     ITAVLASFGSNRSAKQAAAAHVMFNIIGVTWALLTLPLFQSIVMVTAKDVSHQLANAH
                     TLFNILNTLFFLPFTAPFARLIQWILPSSESATPSGPRFLDAKLLKASPAAAVDAVRQ
                     EIVHMGSLAQLMLKDVLQAFLENDHRMISQVNETEKAVNELNHAISGYASELWQEGLS
                     DELSVALSSYVNGVGDIERMGDHAQNLIELFEYKEEHGLQFSKEGREELQDMFATVTL
                     AVKLCLEAMAEENVAKAREVIDHLEEEIDRKEKTLRRQHIIRLNRGICQPSAGVVFID
                     ILSNLERIGDHAHNVAYIVFDIARLHHDVPEA"
     gene            35893..36489
                     /locus_tag="PL162_03355"
     CDS             35893..36489
                     /locus_tag="PL162_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HQF91099.1"
                     /translation="MKENPLDFSPIPRGQKGLSPHHMALVALFAVLTAVGAFIRVPLP
                     FVPFTLQFFFCALGGLLLGSRLGSYAQVVYMAMGLLGLPVFSGGGGIQSLLRPSFGYI
                     VGFVAAAWVIGRLGEREKLPWTFWSATRATLAGLGVLYLVGVPWLWAVYSWYLNDPKP
                     LSWAVTYGFLIFLGGDLVKTALAAFVACRMGVLRKGRG"
BASE COUNT         7104 a        11804 c        10159 g         7470 t
ORIGIN      
        1 tccccgttcc cccgataccg ccgccagccg cccttcgctt cccccttcgt cggggacgta
       61 cgtgccgggg gtcaccaccc gcaccacccg gcgctccacc agggatcctc ccccctgagg
      121 atccccgatc tgctcgcaga tggccacgcg gtgcccccct cgaaccaggc gccccaggta
      181 ggaatccacc gcatggcccg ggaccccggc catggggatc cggcgctcgg gatcccgggc
      241 cgtgagggcg atgtccagga cctcggcggc gacccgggcg tcatcgaaga acatttcgta
      301 aaaatctccc atgcgaaaga atagcaggca atcggggtac cgctccttcc actccgtgta
      361 ctggcggagc atgggagtca tcttcacgtc ttcggggagc atcaggaggc gttccttcgg
      421 cggtctaggt aaccctggag gagtaatgcg gcggcaacac catccacttt gcggcgccgg
      481 tcgcccctgg aggcatcccc ttcgcgcatg gcccggtggg ccatcactgt agtgaatcgc
      541 tcgtcctccg tctccacggt caccccggga aagcgctctc ggagcagagc caccaattcc
      601 atcatccggc gtgcctccgg tccctcttct ccggaggtgc gccgggggaa cccaaccagg
      661 atccggtctg cggcgtagga agtcattagc tcttccaagc ggcgcatcca gggtccttcg
      721 gcgggtaaaa cggcgatccc ctgggcaaag acgcccaggg gatcgctcac cgccacgccg
      781 atgcgaaccg atcccatgtc caaggcgatg atccgcccca tcatgccccc aaaagagcca
      841 tcacgatgcc cggaacggcc tccagggcct ccgaagttcg ctccacctgc ttccctccgg
      901 cctgggccag atcggccttg ccccccccac ctccgccggt ccgggccacc gtctctttga
      961 cgatgcggcc cgcgtgggcc ccttgagcct gggcttcggc gtccgccatg gccacgaatt
     1021 gcacccggtc ggcgaagcgg cccgccagaa ccaccaccac cccggatagg gatcgcttga
     1081 tccgatcccc cacctggagg agcaagtccg cttccgcctc gtcgaaggcc ccggtgatca
     1141 cgctcagagc ccccacggtc accgccttct ccatgagcga aggcacggcg gcgataaaac
     1201 ccctcagccg agcctctcca agagcccggg aaagatgttt gatctcttcc tggagccctt
     1261 ccacccgcaa gggaagctct tcgacttcga cgtttagcat actccgcagg tggtgcacgc
     1321 tccgggaaag atcttggtgg tgccgcaggg cagagatccc cgccacggcg gtgagacgcc
     1381 gtacccccgc tccgatgccc tcttctcgga ggatctttac ggcgccgatc tcccccgtgg
     1441 cgcccacgtg ggtaccgccg cagagttcgg cgcacgtccc gggcatggcc acaaggcgca
     1501 cttcgtcgcc atatttctcg tcaaataggg ccttggcccc tttgtctcgg gccttatcca
     1561 acgtggtgat ttccaccgtc actggggtgt tgcgcatcac ctcttcgttg acgaagagct
     1621 caatggcctc caggtcgtcg gtccttaggg gctcgaagtg ggtaaaatca aagcggagga
     1681 attcgtcgct caccaaggat ccggcctggc gcacgtgggc ccccagtcgc cggaccaggg
     1741 cctcgtggat cagatgggtg gcggaatggt gacgccggat gccccggcgc cggttcccgt
     1801 ccacggcggc cacaacaccg ccgcccacgg cgagggcccc ggagtctact ttgccccggt
     1861 gaatcaccag ctctcccgct ccataggtgg tgttcatcac gatgactgaa gctcccgggt
     1921 ggtggatctc ccccgtgtcc cccaattgcc ccccgcgctc agcgtaaaag ggcgtttcgg
     1981 cgaggacaat ttccacctcg tccccctcat tgacctccgc caccctctct ccgtctttta
     2041 acagggccag tacggaggtc ttggcactct cctgatcgta acctaaaaag agcgtcttgc
     2101 caaactcgtt gagcatttcc gtgtagacgt tgcccctgag ggaggagctc ctctgtttgc
     2161 tggaagcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnagg aggcgccgta
     2281 gcacgtaccc tcgcccctcg ttggcgggga gaatcccgtc ggcgatcatg aaactcacgg
     2341 ccctcaggtg atcggcaatg acccgaagag ccatggtctc ccgaggagaa actccgtagg
     2401 atactcccgt caccgtagcc gctgcctgaa taaggggaga gaagagatcc gtttcaaaat
     2461 ccgtcctcac ccgctgcacc accgaggaaa ggcgctctag ccccatgccc gtgtcgatgt
     2521 ttttctttgg cagggggatg agctcccccg cttcgttccg gttgtactgc ataaaaacca
     2581 ggttccaaat ctccaggtag cgatcacaac tgcaccccgg ggcacagtcg ggcctgccgc
     2641 aagaaaaatc gggcccctga tcgtacagga tctcggagca ggggccgcag gggcccacgg
     2701 gccccacagc ccagaagttg tcgtcggccc ccagacggct gattcgggac tgaggaagtc
     2761 ccacggcctg gtgccatagg tcggcggcct cgtcatcgtc caggtagacc gtggcgtaga
     2821 ggtgatcggg atccagccct atccgatcgg tgagaaactc ccaggcccag gggatggcgt
     2881 cctttttgaa gtaatctcca aagctgaaat ttcccagcat ctcgaaaaaa gtgtgatgcc
     2941 gggcggtgcg ccccacgttg tcgatgtcgt tggtgcggac gcacttttgg gacgtggtgg
     3001 ctctcttcac ttccggcgtg cggattccca agaaaaatgg cttgaagggc accatgcccg
     3061 ctatggtaaa gaggatggtg ggatcttcgg ggcgcaggga aaaactggga tagcgccggg
     3121 acccctttcc ttcaaaataa gacaaaaaca gctcccgcaa ctccccgccg cttcggtata
     3181 tcataggcac gcccccctcg ttcctctagt ccttctgatc tttcacgagc tctcgacggc
     3241 aacgagagct ctctcttttc acctgcgcaa tgtccatcgt gacgaaattg ttctccctgt
     3301 agatccactt cccctgcacc atggtacccc gtacgtccgc cgaggacccg gcgtaaacta
     3361 ggtaagggca cagcgtctcc ggggtccatc ccacgtagtg gggaaggtcc agatccacca
     3421 ggaccagatc ggcctgccac ccctcgcgca ggagccccac atcgtcaaac cccagggccc
     3481 gggccccttc cacggtggcc atgcgtagca ccgatgtcgc cgggaccgca acgggatccc
     3541 gggaaagccc cttgtggagc aatgccgcca gacgcatttc tccccagaca tccagacgat
     3601 tgttgctggc agccccgtcc gatcccaggg ccaggtgaac tccctgggcc aacatggcgg
     3661 gaaccggagc cacgccgcta ccgagcttca tgttgctccc ggggttgtgg gccacggtga
     3721 ggttggggcg ggccaggtcc ttcagccgat cgacgggaaa atgaaccccg tgcgccaaca
     3781 ggaggcgagg ggcctccagc aggcccactt cgtccaggag gtccacgggg tctttcccca
     3841 gggtatttct tatgtaatcc agctcccagg tcgtttcaag ccagtgaatg tggactcccg
     3901 ccccgcagtc ctttgccagt tctgcgatct gcttcaagcg ttccgggggc accgtatagg
     3961 gggcatgggg gccgtactgg acggagatgc gatcctggca gccgtgccaa gcacggaaaa
     4021 gttcttcttg ctcttttagg cgccgatcgt tttcccccgg ggagtcgccc catactattc
     4081 cccgagagag accgcatcgg aggccgctct ccaaggctcc tcgggccact tcgtccataa
     4141 aaaagtacat gtcgccgaag cacgtgattc cctgggaggc catttccgcg atggccaaca
     4201 tcgttcccca gtagacgtgg tcctctttaa ggcgggcttc cacgggccag acggcctgct
     4261 gtaaccactc catgaggggg cgttcttccc ccaacccccg gagcaacgtc atggcggcgt
     4321 gggtatgggc gttcaccagg ccaggaatga gggcccggcg tccccccccg cccaccacct
     4381 cgtcatcccg cccttgcccc ggggggagga tggcggcgat gcgcccctcg gccacgagga
     4441 cgtctccata tacccccgaa ggaaggtctg cgtcgaccat acaaacgttt cgaaaaagca
     4501 tggccacacc ccctttagtt tttttcgtcg acctcgtttc cctttcgccc tggaggaagg
     4561 accgcccgaa cgaagctgtc cggagaagat tttattccgg cggcgaagat cccgtaagag
     4621 ggcggcggaa caaaagtcga agggatgaca ctttcacagc tctgcgtctg agcttctcat
     4681 cccggggtcg acggcacggc aaagtatcca ggcccttttg cctttcagtt ccgcaaaaaa
     4741 attcttcgaa aaatggttaa agggccgcag aatcgatatg ccaaatcaat agaaagggat
     4801 ctcggtcgac ggctttactc tttccagctc gacaggtagg cttcctgttc gggggtcaag
     4861 cgctccacag ccagccccag agaggcgagc ttcgacaggg ccacctggcg gtccaagtcg
     4921 tcggggaccc ggtgtacccc tggagggagc tctgtgtggc agaggtgcag gaccgaaagg
     4981 agttgcaacg caaaactcat gtccatgatc tcgatgggat gtccatcccc cgccgccaag
     5041 ttgaccaggc gcccttccgc caaaaggtgc aggcgacggc cgtcgcgcag cccataggtc
     5101 gtaacgttgg ggcgatagct ttccacgtgc tcggatcggg ccaagaggtc gggcttggat
     5161 atttccacat cgaaatgccc cgcgttggcc aggagggccc catctttcat gacatcaaag
     5221 tgttcccccc tcaggacatg aatgttcccc gtaacggtga tgaagacgtc tcccttccgg
     5281 gcggcgtcga tcatggaggc gaccccgtgg ccgtccataa gggcctccag tgccctatgg
     5341 gggtcggcct ccaccaccac caccgaggcc cccaggccct gggctcgggc ggccactccc
     5401 cggccgcacc acccataccc caccaccacc acggtctttc ccgccaccac catgttggtg
     5461 gtgcgcatca ccgcgtccca gaccgactgc cctgtgccgt aacggttgtc gaagagatac
     5521 ttgctgaagg cgtcgttcac cgccagcatg ggaaatttga gaaccccctc gagttccatg
     5581 gcccgaaggc gtttcacccc ggtggtggtc tcttcgcatc cccctcgaat gcggggcaac
     5641 aggtccggac gctcttcgtg caataaggat accaggtctc ccccgtcgtc gatgatgata
     5701 tcgggatcga aagaaatggc gtcccggagg taccgacaat aggtctccgg gtccatgccc
     5761 cggtggctgt atacggcaat gccttcttct accagggcgg cacagacatc gtcctgggtc
     5821 gaaagggggt tgctccctgc ggcggccacc tcggccccca ggagcttaag agtcttcaag
     5881 aggcacgccg ttttcgcctc caggtgaaga cagaccgtca ctctcctccc cgccaaaggc
     5941 ttggaaggac cgtgcttctg ggcaagataa cgaagaacgg gcatggcatc ccaggcccaa
     6001 tggatctttt cctggccccg gggggcttgt tcggggtggg caatgcgtga cgcagtcatg
     6061 tatgcttcct ccctcgtgtt ttaaagtccc tatcgacggg cacattctac aggagcgcgc
     6121 ccttctcctc aataatcgtc gtcgcaaaaa agtgccgctc tacgatggac tgggaaagcc
     6181 tctctccccg gcggcttatg aagtcaggat tcccaataga tgtcatcttc cggtacgaag
     6241 ggtctatctc gggcctcttt taggctttga tcgaaggggg ggtgaaggac ccccacttcg
     6301 gtgatgaagg cactgaccag atcacccggg gtgacgtcga aggcgggatt ccacacggaa
     6361 gccccggggg ccaccacgat cccgtcgccc catcggcgca cttcctcgtc ttttctgatc
     6421 tctatgggaa tatcggcccc cgtggcggtg taaaaatcca ccgtgctccg aggcatcgcc
     6481 acgtaaaagg gtaccccatg gtaccgcgcc gccaccgcca ctccgtaggt gccgatcttg
     6541 ttggccacgt ccccattggc cgctacccgg tccgctccca cgatcaccgc cgacagggga
     6601 aaccgggaaa aaagggcggc ggccatgccg tcgcagatga cggtgacgtc catcccctct
     6661 tgggatagct cccaggaggt gagccgggcc ccctggagca cgggccgggt ctcatcggca
     6721 aaaattttta accgctttcc ttgatctcgg gcgctgcgaa gcacccccag ggcggtacca
     6781 tgccctcccg tggctaaagc cccggcgttg caatgcgtca gcaccatgcc ctcgtcgggg
     6841 agtagggcgg ctccaaagga tcccatggcc cggttgacgg cccggtcctc gcgttccagg
     6901 cgacacgcca gctcttccag gcgctggatt cgttcctggc gccccatcct ctcccccatc
     6961 gcctcccact gatcgcgcat gcgacccaag gcccagaaga ggtttaccgc cgtgggcctt
     7021 gtccgggcca atcgggccac cacgcggtcc aggtcttctc cccgttttac ccccaggacc
     7081 accccatagg ccgccgccag gcctatggca ggagcccccc ggagggccag agcctcgatg
     7141 gcggaggcca cagcctcggg gtcatcgcag cggatccact ccaccgaacc cggaagacgg
     7201 cgctgatcca atatttccag ccggtctttt ttccatacga ggggaacaat catggcttta
     7261 tccctcgctc aaaggcctcg gaggggggaa tagcccgttc caccgcctcg acttgtgcca
     7321 gagccacggc atccatgaag cggagcctta ggcggtcccc cgggaacagc tcctctcctc
     7381 ggacgagggg agtgccgtct tccctctcgc agaagggaaa tcccctcctc aagggatgcc
     7441 agggcgaaag ggcttcaagg cgttcttcca tgagatccag cgccttaccg actccatcca
     7501 gagcacttcc cagggctgct ccgtgtcgct ggatcaggcg atccaaggtc ttttctcctt
     7561 cttttaagta acttctcacc gctcgagacc ccctctgcgt ccattggtcc atctgtaaag
     7621 aggcgcgatc atgggagcgg gaaagggcca tgtgcccccg gcgcgccaga gattccaggc
     7681 gatccaagat ctcttgtcgg tcgggaagga gcttttccac cgccgccgag ggcgtgggtg
     7741 tcgaggcgtc ggccaccatg tcgcagaggg acagatccgt ctggtgtccg atcccggtca
     7801 ccagaggcac gggagagtgg cgcgcggccc gtaccacttg ctcgtcgtcg aagggggaaa
     7861 ggtcttcccg ggctccccct ccgcgtacca acataatggc ctccactccg ggcacatgcc
     7921 ccgcgcgccg cagggctcga accagagacg cgggagctcc caacccctgg accagggcgg
     7981 gcaccacaaa aaaacggcag gaaggaaagc gcttcccccc taccttgatg acatcctgca
     8041 ccgccgcccc cgtgggggag gtcactagcg ccaccacctc cgggaaaggg ggcaagggcc
     8101 ttctgaggcg cggatccaaa agcccctcac gctccaaaag gcgacgcaac tcctcccggg
     8161 ctcgactctg ggcccccgcc cccaggggga ttaaccgcaa ggcataaatc tgataaattc
     8221 cccgggcttc gtagacggcg atgcgcccct ccaccaggac ggcatcccct acccggggcc
     8281 aagatatctg tctgaaggcg tcgccgcgaa agagcactcc ccccagtcgg ctttcccctt
     8341 cggacaatgt aaagtaagta tgcccgctgg agtgtctttt tatttccgac agctccccac
     8401 ggacccaaag gtgacgcaag aggaggtctt cggaaaacct ctgggagaga taggctccca
     8461 actcgcagac ggaaagggac cggggctcca ctagggctcc tcgccggagg gggattcctg
     8521 ggcccgcaca attttcccca gtacgccatt cacaaagcgc ccagagtctt cggtgccgta
     8581 ctgcttggcg agttccatgg cctcggagat ggccaccgct aggggggtct tgcgggccac
     8641 cacaccttca aagagggcca accgcaccgc cgctcgatcc acagccacca tacgctccgg
     8701 gcgccatccc accacgtgcg tccggatcat ctggtcaatc tcgatcctat ggtcccaagt
     8761 tccctccaga aggtcgcggg catactcttc cacgccatct tcttcgtcga agtcctgcag
     8821 cgcccacagc gacgcaggag tttcttcggt gcggaggtcc agcgcgtaga gcaactgcag
     8881 ggcgatctcc ctcgatcgcc gccggcgctg gggtaacacg gcctgtccca aaaacttacc
     8941 cccctcttac gagaggcgaa gcggcgcccc tatttttagt ctaccggggg cgcggagccc
     9001 cgccatacca aagatctatc gccttccgac aaaagatctt ccacttgtcc tgtccccggg
     9061 gggtgagcac cagccacgcg acgccaaagg cccctcctcc ggcaatcagg gcaccgaagg
     9121 ttccgtccca tcccaggcgc cccaccgcca ccagaacccc ggcagccact ccccacgtct
     9181 cggggcgccc catccaccgg cgccaagagt gatcttccac ttctccctct tccgtccagg
     9241 atacttctac gacgccgaat cccaaggggg caaggaaagc ccgtacatcg tcccccacgg
     9301 ccctcagggc ttcatcggag acccccccct cgccgttcgc gtaccccagg cgaacggcgg
     9361 cggtctgccg ctccttccaa aaatctacgg cccggactac gcagcgctct gggagcatgc
     9421 cctcaaggag ctggcgcacc cctcggctct cgatccaaag gttgccccga tcggtttgtt
     9481 gccaccacag gaacatctgc ctcacccctt gagcggtttc tgtaagacgg caacgggatg
     9541 gcccgatcta cacgccctca tcggctctgg cgccagaaga agggcatcct tctttgctga
     9601 tgctgagggt ggggccgtcg tctcccgagt ttagggtgcc cgccgccgct ccgtctccca
     9661 ccgtcgccgc cggagcctcc gcttcgacgt cgttgaggta gatgctctgg acgaagacgt
     9721 tcacggactt cactcgatat ccggtaaact tttcaagatg attttttacc aattcttgca
     9781 cgtcccacgc cagatcgggg attcggaggc catacttcac cgccacatag gcgtccacaa
     9841 ggatttgagg aggggtttta tcgtccaggg agacgcgcat ccctccgctg gtctttctcc
     9901 ccagcccaag ggacgccagg ggactggctc cggcgggacg gacgccctgg acgctgcgta
     9961 gcgcttgagt ggcgagctgg aggatcacat cttccgagat gcgaacctct ccccctcggg
    10021 gcgcggcgga ttcctctttc tcctcagatt ccgtaaagtc ttgatcctcc aaaatctcct
    10081 cttcgcgcat ctgcagatct cgttccttct cttccatgcg gtacaacccc cttcttatgt
    10141 gggctttccg gtaaaccatg gcgcatcaaa gggcgcccgg gcatcccggg aggcgttact
    10201 tcttggctcg ctccagatag agccccgtac gggtgtccac caccaccacc tcgtcggggt
    10261 tgatgaacat gggcacggtg atcgacaatc ccgtctccaa ggtggccggc ttccctccgc
    10321 cggacaccgt atcccctttg aatccaggct ccgtctgcgt cacgcggagc tccacggatt
    10381 taggaagttc gatctccatg acccgacctt cgtgcatgtc gatcttcact tccaggtttt
    10441 ccgtgaggta cgacgccatg tcccccagga tctccgggga aagggacagc tgatcatagc
    10501 tctccaggtc cataaagaca aacagatccc cctcacgata gagaaactgg gcgggtttct
    10561 ggtcaaagat gatgcgctcg aagcgctctc ccgaccggaa tgacgtttcc acgatggatc
    10621 ctgtcaagag gtttttcgcc ttggttttca ccgtagctcc gccgcgaccc attttatggt
    10681 gctggtaatc aagaatctcc caaatcccgt cctgccatcg gaccttaagc cccacaaaaa
    10741 aatcgctggt atcgacaatc tgccccataa actcccatcc tcctaaaccg tcggaatcaa
    10801 ccgatcgatt atagcagccc ctccacccct ctgaccaccg tcgtacccct tacgtcgcaa
    10861 attcgctttg gcgcgtcgtg gaaaaccgac gatcagggga tcaaaaaacc gaccccgaag
    10921 gagcttttgc ggcggttcta cctaacgatc cccgcagaca ccgacggctc tccccccgcc
    10981 gtagctcaat tatttgtccc cctcacggat ttctccgatg ggcggtgggg gcgaaaagtt
    11041 catcgctttc caccggagca ttgggaatgt ccaggatgta aggaatcacg atcatggcca
    11101 cctcggattt tttatgactc ttgctctccc atcggaagat ctcccccaga aggggaagat
    11161 tgcccagaac ggggaaggag gtcttttggg tcgacgtcac ttcctggaat agtcccccca
    11221 ccacaaaggg ctccccattt cggactcgaa cgttggtcgt caccgaccgc gtgctggtgg
    11281 cgggggtatc cccgttggag aaatccaatg tgccggtgga tagggtcatc tccaagttta
    11341 ccaacccgtc tcgaccgatc cggggggtga tgtccatctt gggtcccgct gtggcggtgg
    11401 agtataccgg gttgtcgtcc ttgtacgagg agatataggg caccgaggtg gtgaggtcta
    11461 tggtggcttt catgccgtcc acggtcacca ccgaggggcg ggccagattt ttcccttgac
    11521 cgtcagtctg cagggcatta aggcctgcgt ccaaaaggcg cagagctccc tgggataggt
    11581 cttcgggatc gaggccgtcc aagatctggc gctccggatc gtacttaggg ggcttattgg
    11641 tgtccaggta tccgatgctc atgcccccct ggccataggt cacgtaccag tgcttgtaga
    11701 cgtgggcgat gacgctctgg agcttgtctt cgttcccgtg actcacctcg aagatccggg
    11761 cctgaatcat gacctggcgc ccgggatgat cgatgcgctc caaggtctgg gccacctcgt
    11821 tcaggatgtc ctccccagcg gtgacataga tctcccggag tcgagcatcc acggtgacct
    11881 tatccactcg caccaatcct tgtagaaggg gtaccacgtc ctccggtttg gcataggcga
    11941 cacgaaacac tcgggtgctc tctttcccca gggcccgggc caactggccg ctttttccca
    12001 ggatcaagga gttccccatg tacgcatact gcacgtcata catgcgcatg aggtaggcga
    12061 aggcctcccg catggggacg tctttcaggc tcaccgtcac cgcagccgaa gggaaggaag
    12121 gatcgcagat gatgttgagc cccaacatct ttcccaacat aaaaaagact tgttttaggt
    12181 cttcttctcg tagctctagc gtcaccggct tgttgctata gagcggatct ccgccccggg
    12241 ctaccgctgg gactcctgag ggcgtggcgg gccccacagt gagaggtttc gtggcgggct
    12301 taacctccaa aaagatttca agctggttgg ccggagccac tcccttagcc tggcgcaccc
    12361 gaaggggatg gttcgtttca aagacaaggg ccatccctcg ctcctgctgc tccatgcgca
    12421 cctttttcac caaaggcacg tcgtcgtagg ccctttccca agtcccggaa gggatggaaa
    12481 gctttgggaa aaagagctcc acctcgtcgg ggtcctctcg ctgcaacacg ggaaggggta
    12541 atgaggatcc cttaatgtga cacagcaccg ctttatcccc aagctgctgg atttccaccc
    12601 cccccaccac gggcaacaag gggcctcctt ccgcccccca agcgaaagct agcaccccta
    12661 ggagtagggc caagccccct ccgaagcccc agcccttcct cggggccgcc acgccagtgc
    12721 cccgggtcgc gccaacaaaa cgccggacag tcatcgggga tcgatgacta tttccatgcg
    12781 cttgcgcatc catacgacca ccaccctgtc tttcgagatt ttaagaattc gtccgtgccc
    12841 cttgtcgaac ttcatccctt ccgtcacgat gatgcccgtt ccttccgtct cgatatccat
    12901 caccgccgct cttcggtctt ccaggatcat gatggaccgg accgtcatga gaggtggtag
    12961 ggcctccgcc acgggcacca gatcgctggc ggcggtctcc cctacgttgg ggcggtaagg
    13021 ttggcggtgg gtggcctcca tgatggtcac cgtgtccttg ccccccgtgc gcaaagtcat
    13081 cacgccatcg aagacccgca ggagctcctc gactcttccc tgctccccta tggtgttcac
    13141 gggaggggca aacaagggaa cggggtcttc tagctccacc atcatgtcgt gcccagattt
    13201 aatctgccac gccgctcctg ccaggagaag caacaccaaa ccataccgca ttacctgaga
    13261 caggacggta tcgccgctcc ccaggcgccc ccaaagggcg tccagggctt ctccccacgt
    13321 ttcttgggcg ctcatcagct ttctcctccc gcccgccggg gcgacagacc aatgagggtc
    13381 tccaggacgg tattggcttg cacatctccc tttcgatcca tgttaaaatt gatagtgctc
    13441 atgcgcacca aggaagagag ggcgcgccac tccgccaggg cgcgcatcac cttgtagtag
    13501 ggccctcgga actgaacggc tacggagatc ttcccctccg gagaaactcc ggcagtagtc
    13561 atgctgatct tttccaatcc ctcttgggtt agcacctgct ccaccgccga cagaaaggcc
    13621 acctgatccc ctggaaagcc cacctgggac ttttccagcg aagccaaggt ttttttgtag
    13681 agctggatca aggctgcgtt cgtctccgag gaatggcggt actgcacctc ctgcctctga
    13741 agctccctct gccgatcaat attttcttgg agcttccaac gtagcaccgt ggtgccccct
    13801 agccctccac ctaccgtcaa cagtagcgcc aatcccagta ctatccgttt agtacccatg
    13861 actttccggc ctcctcaacg gggtgtgatc agcgccgtta ggtcgcggag cttgcaggtc
    13921 atggtgaatc tcaccgtgcg ctttaatccc tcgccgcttt gagatatcac cggcactaag
    13981 acttcggcta ccacggcggc gttagccagc gcgttgctga atatgaccac atcgccttcg
    14041 gaaaaaccca agcccgtgag agtggcggcc ccggccacga catcgactcg ttcaatccac
    14101 accgtaccgg gtagagcccc ttccatggcc cccaagagct ccagggcggt tagctcctcc
    14161 ttgaccaagt cccgggcccc ttcgtagaag gcgatttcct gctggagatc ctggacggtg
    14221 cgggccagct tgccgtcctg ggcttgcagt gccgcgacgc tgctttccag atccatcaaa
    14281 gacagagaca gggcgtggag ccgaaaggcc tgatagcccg ccacgccgcc gctagaaagc
    14341 atcatcaaaa ggagcgccag gaaaaagagt atcttgcctc ggtccaccgc gtaacgcttc
    14401 tgagtctggc ggtactgagg agggcgcagg tcaaaacgga tcgtcatggc tcgcctccca
    14461 ccaaaagccc caacgctcct tcccactccc ctccggaggg aggattggaa atgccccact
    14521 ggtcccacat cgccaccgct tgtaccgcca cgtcgggcat gatggagcgg atttcttcgt
    14581 caaggccggg ttcccgggaa aactcccccc ccaggaggac ggtatcgacg gaaagttccc
    14641 ggaactgatt tcgcacgaag gccgccgtgg atcccgcctc ccggccgatg gtacgcacca
    14701 tctcctcccg gtctcccccg gctccgaagc ccaccaaaag ggttcgaaag agcaagccgc
    14761 tggagcgata ggccaccata atctgagtgc tttgcttctc cgggagcacc aagagagctc
    14821 ccgatttgaa agaaggcatg gggcctagga gcgaccgaaa aatacccacc agtatgggtt
    14881 ctgccccccc cacagaaaag ccacctttga cgacgatgtc catcaccgtt tcgaccatac
    14941 gaaggcgggc cgccgccacg agaactttca tcttctctgc atcttcgcct ccgggaacct
    15001 ccagggggca cacgtcgtac accgcttctc ccgcagggaa agggaagtac ttgtcaaaat
    15061 cccactgtag cgcatctcgt gcttcttctg cgcccatcct aggcatgtcc accacccgga
    15121 gcataacatc tcgggcgggc aacgagatgc tgacgggaac tttcaggaac gacccatcca
    15181 tctcctgccg aagtttttcc agcagaggca ccgcccggtt tgcatcgggc aacatcccct
    15241 gttgcaaaaa gggatggggg gtcgttacgg ccttagcctg ggtcagctta aagtgccccg
    15301 ttgttccgtc cagggccaaa tatctcaggc cgtcgctaac cagggaaagg gatgcgcgag
    15361 ccgtattctt ccgccacagc gccaaaggct ttcaccttcc ttcgggggat acgaagaatt
    15421 ttactgcggg agggagaaac cccctcggct ccatcttagc acagaaaaga gatctcacaa
    15481 aaagcacttt tcccccccgc agatatgggg cgatctgacc aataaactgc caaattggct
    15541 tgacgaaaag catagctcac ccctccaccg ggaacctcgg cgacctaaaa agagccgccc
    15601 ggaaaggggc ttcgcaacgc cgttagcgga tgaggctctc tcacgaggat aaatagcaac
    15661 gcccccgagt ttaaataccc tccctggcgc gcgaagtcgt gatcttaggt atcgcaaaag
    15721 ggcctccggc aaataccttc ccgaggcccc cttggggaaa gactagatgg cgcctccagt
    15781 cacggaaagc acctattcgt tcatgatttc tttttctttt tcttctagaa ccgaatcgat
    15841 ctgcttgatt accgcatccg tggagtcttg gagttccttc tgatacttgt gtagagcgtc
    15901 ttccgtgatc ttggcgtcct tttccatctt tttgaaagtt tcgttcccgt cccgtctcag
    15961 gttgcgcacc gccaccttgg cctcttcggc gtacttgcgg accaacttgg tcaggtccac
    16021 ccgacgctcc cgggtcagct ccggaaggcg gaggcggatg ttttcgccat cgttttgcgg
    16081 agtgatcccc aggggggagg cgagaagggc cttttcgatg gccttgagcg ccgccttctc
    16141 ccagggagag atgagaatct gtcgaggctc gggcacttgg acggtgccga gctgcttgat
    16201 aggggtctga gccccatagt agtcgacttt gatgtcttcc accagggcgg gatgggcgcg
    16261 accggtacga accccgagac actcgacgcg cagatgctcc acggtctttt ccatacgatc
    16321 tctgatctgc ttcaactctt gggcaggcat gtcgtctcct cctctccagc gtagacccta
    16381 gtccccaggc actcgttttc caccagaagt ctcctcaagt tgccacaatg caagatgttg
    16441 aagactacgg tggggatgcg gttttccatg cacagggaaa acgccgccgc gtccatgacc
    16501 ccgatgcgac gcctcaatgc ctccgcgtag gtgatggtcg agaaaaaggc ggcgtcgggg
    16561 tggatccgag ggtccctctc atatatacca tcgaccttcg tagctttcaa caggcaatca
    16621 gcgtggatct ccgccgctcg gagagccgcc gccgtatcgg tggaaaagta gggagatcct
    16681 gtcccggcgg caaaaatcac cacccgcccc ttttccaggt gacgcaccgc ccggcggcgg
    16741 atgaaaggct ccgctacttc acgcatctcg atggccgtct ggacccggca cggcattccc
    16801 gccctctcca aggaatcctg aaaggccaga gcgttgatga cggtggccaa cattcccatg
    16861 ttatccgcct gggcccgttc aatgcccttt ggtgaagctt gggctccccg gaagatgttg
    16921 ccccccccca ccaccatggc gatctctacc ccgcttcgag ccacgtcggc cacttcttcc
    16981 gccagggcgt ccacagtggg aaagtccagt ccaaagcgtt ctccccccgc caagacctct
    17041 cccgaaagtt tgagcagcac ccgacgccac atccccatgg gacctacctc ctcgtcgtac
    17101 gccatttttt caaaaaaaaa ccggcgagga tttttccctc gccggagaaa gactcaatct
    17161 actgcccgat ggaatatcgg ctgaagcgtc gaacgacgat gttttccccc attttggcga
    17221 tctggtccac cacgaggtct ttgatcttcc ggtcggtgtc gcggatgtag gcttgctcca
    17281 ggaggcagct ctcatcgtag aatttcttca cacgtccgtc agcgatcttg tcctggatct
    17341 ggggaggctt gccctcttct tccgcctgtt tgcgataaat ttcccgctct cgttccactt
    17401 caccggcggg cacctcttcc ggagacaggt aaagagggtt cgccgccgca atgtgcatgg
    17461 cgatctcgtg gccgagctga cggaactcgt cggtgcgagc cacaaagtcc gtctcgcagt
    17521 tgagctctac cagcgtgccg attttgccgt tggtgtggat gtagctgaaa accattccct
    17581 gggaagcttc tcgcccggcc ttcttggcgg ccttggccaa gcccttttcc cggaggtaat
    17641 ccacggcctt ttccacatcg ttatggcact cttccagggc ttttttgcaa tccagcaccc
    17701 ccgcgcccgt ccgggcacgg agttctttga ccatttccat ttcccccgcc atgatctgct
    17761 aggcctcctt ccagcctttt cgctcttcca aaccctttac cgccagggtt tcgccctcgt
    17821 cgccgtaggc gtcgtggagc ttttcccgca cctcaatcgc ttcaggagtc tccgccaagg
    17881 gggcgatccc gccgggagtc tccggaacgc tcactccttc cacttcttct gtagcagcct
    17941 tctctttagc cgtcagggga gcgtcggagc cctggttgcc ctcgatgacc gcgtccgcca
    18001 tggctcccac cataagtttg atggctcgaa tggcgtcatc gttgccgggg atggggtaat
    18061 cgatgacctc ggggtcgcag ttcgtgtcta cgatggccac cacgggaatg cccaacttac
    18121 gggcttccat cacggcgata tcctcccgcc gggggtccac cacaaagagg gcgtcgggaa
    18181 cgtctttcat gtcccggatg cccacgagat atttctccag cttggatagt tccttgcgga
    18241 gctccagggc ttcttttttg gggtaaaggt caagcgtgcc gctctcctgg agagactgga
    18301 gttcaaccat acggagcacc cgtcgacgga tggtctggaa gttcgtgagc ataccgccga
    18361 gccaacgctg gttgacgtag tatcccccgc tacgcagggc ctcgtctcgt acggtctcct
    18421 gggcttgacg cttggtaccg acgaagagca cgctccctcc cccggacgag acctcccgca
    18481 gaaaatcgta ggctttttcg agtcccttca cggtcttttg gaggtcgatg atgtaaactc
    18541 cgttacgctc cgtgaatatg aagggcttca tcttcgggtt ccaacgccgg gtctggtgcc
    18601 cgaagtgcac cccgcactcg agcaactgct tcatggaaac aatggccatt tcttttcctc
    18661 ctctcggttt tgtccctccg cggccatcca ccgcctccgg atcccttcag ggcaccgccg
    18721 gagacgtccc tccgcgtgtg aattgcggac caaaagcgat tgtatcacaa aatattgttg
    18781 acccgcaagc gctccgtcgc taagatggtt tgggatgcct ccgtcaccga tgggggcagg
    18841 gtcgacggga cgccttccct tcgccaagat catgggaagc ccccccgcgc tttcccgcca
    18901 aaggaggcct gtgtatggag cgcacttcgc gggtccagga gattgattcc ctgacgccgg
    18961 aaaagaaggc catgattaac cgtctccgac gggtagaagg gcaactccgg ggaatccaac
    19021 ggatgatcga ggaaaactct ccctgcggcc ccatactggt ccaactctcc gccgcccgtc
    19081 gggccatgca ccaggcgtgc atagaggtct taaaaggtca cctgaagggg tgccttaccc
    19141 ggggacaagc tcctgaccct aaggagctgg agcaattgat cggggccctt ttggagctcg
    19201 ccccggcagg ggaccagaac ctttccgaaa aacatgaatg atgatggatt ctaccgaaac
    19261 atcggcctct cctagggagg gtcttgaagc cctccggaag gggacccttc ggatcgcaca
    19321 gaagtcccgg ggatcttttg aaaccacggg ggcaggggta gtttggagga gtttcgcgag
    19381 gtaaaaaaac gggggggctt caagcccccc ctagtcatgt cccttcacgc atcgaccccc
    19441 cgaggggatc ccagcgcccc cgtggcaccc aaagcgatcc gcgtaccgtt gcttccttcc
    19501 ggacctagcg gggttggcgg ttctttgccg cacgggactg gaagcccctc gggggatcgt
    19561 tgaaaaaaat ggcggagagg gcgggatttg aacccgcgag gcaggtttga gcccgcctac
    19621 tcgctctcca ggcgagcgcc ttcgaccact cagccacctc tccgctacga gaaacgcccc
    19681 ggacgggccc tggaagttta ggtctggcgg agagggtggg attcgaaccc acgagacggt
    19741 tacgagccgc ctagacgatt tcgagtcgcc ccccttcgac cactcgggca cccctcctcg
    19801 ccgcttcccg ccccttcaag ggcgcgaccg aacaaagggc attatacacg ctccatcgct
    19861 tccgtcaacc ccctcgggcg gcgactccgg gcccgcaccg tggagaaaaa ctggtcgata
    19921 agggcctggc actcccccga tcgaaggccc cggcgaacga ggcaacggtg aggaagcctt
    19981 ccatccttcc cgaggtcgta aaggcttccc caggcgccga agcgggggtc ggcggccccg
    20041 aaaacaaccc gatccacccg ggaggcgatg agtgccccgg cgcacatgac gcagggctcc
    20101 agggtgacgt agaccgtgca cccgtccaga cgccacgtct gccgccggtc ggcggctcgg
    20161 cgcagggcca ggatttctgc gtgggcggtg ggatcttgtg aggtttctcg ggcatttcgc
    20221 cctcgggata agagcttccc cccgtaaaaa accacagccc ccacgggggg ctctccttct
    20281 tccagggccc ttcgggcctc ttccagggcc agcgccatgc cctcttcgtc cttgagttcg
    20341 accgacggga agctagacat gggggatgtt gcgccccaca aaaatttcgt acatctctcg
    20401 ttttaacgct tgttttatct gttttttctc ccggggtaaa agatcttttt tccccccttc
    20461 gaagagataa ttatctagat cgaattccct taaggccatt tttgtatgaa atatattttc
    20521 ctggtaaacg tttacgtcga tcatttggta tcgatctctt gtttcgctag agatgtagtt
    20581 ttgaatagaa tttattttgt gatctataaa aatcttttca ccatccaccg ccctggtgaa
    20641 tcctcttaca cggtagtcga tgatggcaat gtcggggtca aaggcgtgga tgaggtaatt
    20701 cagggctttg agcggagaaa tgcggccgca ggtcgacacg tccacgtccg ctcggaaggt
    20761 gctgactccc ttgtggggat ggctctcagg gtaggtgtgg acggtgatgt gactcttgtc
    20821 caagtgggcc accacggctt cggggagggg accggggctt tctccttctt ggtcttctcc
    20881 cccttcttgg tcatggtcgc ctttttccgt ctcttccatt ttttcctcgg agatgagcat
    20941 ggtaacgctg gctccttggg ggtcgtaatc ttggctggcg atgttgagca cctgggctcc
    21001 tacgatgtga ctcacctcca tcaggatgga ggtgagtcgc tccgcgttgt actcttcgtc
    21061 gatgtacgcg atgtaatcgc gacggtgctg aggggtcttg gcgtagcaga tgtcatacat
    21121 gttaaaactt aaggtcttgg ttaggttgtt gaaaccatag agtttcattt tttttacctt
    21181 tttcactggc ttgatttcca aaaccgtctc atcctccttt cggcacccga accaccagct
    21241 ccgggcccct agggcatctg gcgctcgatc acccgcacca ggtcttccac ggcagttttc
    21301 cccaataccc aggtcttgca gacgttgtct ttgtcaatca acaccatgta agggacttcg
    21361 tccaccttgt actccagcgt ggcggagccg tagtcgtcaa tgcaagccac cgtcgctccc
    21421 agccctgcct tgatggcatc ccaagaggcg tccccgtatt tggcccgcat ctgtccatcg
    21481 tcgtaagcgg gggcgatgga caggatcgaa aggtcctcct ccgagaaccg ggcgcgcaac
    21541 tcgggaagag ccttgatttc atccaggcac tccgggcaag tggcgctcca aaacaccaaa
    21601 agggtcaggg tccccggggc gaggtcccgg atccgggccg cccgctgctc ttgagggagg
    21661 aaaaggctgg aattgggcag cttgtcccct accttcaccg ccatgcccac gtagtccacc
    21721 cgctgctccg tgggacacac ccgttctaag gccccggcct caccgcttgc ccccagggcc
    21781 caaagaaaaa ccaaaagtct caccatccac cgcaccgtgc cccatcccct ttcgattttt
    21841 ttagccccta tacattggtg tcgctacccg tcgcggcaac aatcgatgaa agcctggacc
    21901 aatcgggaca agcccttctt cgaattcaac ggcgggacga catcgcttcg aagtcccacc
    21961 cccacgcccc ccggcagggc ctgggggagg ggaattcgtc cttcccggct ccggggaggg
    22021 agggaagcgc ccttttcccc tgggcccgtg aaggcccaaa acatggacca cagggtaaag
    22081 gcctcttcga tgagccgatg ggacatctcc tcctgaccct cggcctggcg cttgttggcc
    22141 acttcgagga ggcgcatggc cgtagccaat aggtgtttgg agatgcacca cccctcgccg
    22201 taaccctcgc gcaccacttg cttcatgaga tctcgcctta agtttcgaat gtccggcagg
    22261 gcttcgacga aaaagggatc tccggtgcgg gcgaagctac acgccagatg ctcctcaatg
    22321 gagatgaggt tcatcagggc aaaggaaagg tcttcccccg aagaaagatc ggaaaaaccc
    22381 tgggttcgca gggcttgctc caggcgctcc tgccagatct tgccgccttc gatccccgaa
    22441 agctcttcgg aggccggaag tccttcggaa gggtgcggat cctgtaccgt ggtcgtcata
    22501 tctcctaacc tttctgcgtc catggtgcgt cagacgcctc tccagtatcc caccaaagcg
    22561 ggtaccgcac aaactaaggc catggccacc gccgacaccg ccacggtctg aaaggggaaa
    22621 agggtccttc cgaaggccct cttctgggcc ttgctcagca ggatggccac gaagagcacc
    22681 agggcgccga accacgccca ggccgccaga ggagaaaaga tcagaagaaa aagaagccgt
    22741 tgccaggtca ccccgttgcc aggaatcatg atcatgttat tgaaaaacac cataagcgcc
    22801 gtgaagacca caggacgata ggggaaacga taacggcgca gggcgtaatg catccacagc
    22861 gccgccactg ccgaaagccc ccccgtcagc gcccccacca cctccaatct caaaccgaag
    22921 tacttggcaa ggagaagtcc cactcctgcc gtgacaaaac acagcgggca agaagcttcg
    22981 gcggcctttg cccccagcgc tcccgccgcc accgccgtcc ccgcactcca gggcacgatt
    23041 ctcctcatgg atccttgcgc tcccatctcc tcgaacgagg cgtcatctcc ccccatagtg
    23101 aaggccttca ccgcctttgt catccgccta ctggcctaat ttatcctgaa agcgcaaggc
    23161 taagaccagc ctcccgaggg gggctggcct taggtactct ccctagctcc ccgacattcc
    23221 cccgtcgggg gtataacaga tcactcgagg tccgctgtgg cgttggaacc gtcccagtgg
    23281 agcgtcaccc gggagatgtc gcaaagattg agcccggtcc actccagggc gttgccgtcg
    23341 gtgtcttcga tgcgcagatc ccactgacaa tgatcaaagc cggaaaactt gacggtgacg
    23401 ctctcccccg acgccagagt gtccaccccc aagatgtctt cctgccagtt gttctcgctg
    23461 gagggagagc agtacaccgc attgatgacc acgccggtgt cgtttaccaa cgtaaaatcc
    23521 tgagttcccg cccaagccaa cccggcccat gccaataggg ccaccaaaac cactccacac
    23581 gccaacttct tcaaccgcaa catcctcctt ttgaggaaaa aatatgtgac gtcccgtatt
    23641 atagcacccc ggggcaaaaa cggtgaagcc ccccatcgag atgtccccac aggccgtcgt
    23701 ttcccggggg cataggaggc tgacgcgcct tcgatggtct ctccgggctc cttcatttct
    23761 atgattcgcg cccccctagt cgtgggtccc ctcttctgga caaatcgccc tcgcctcttc
    23821 ggggggaggc gcaaggtaga tcagggcgaa ggggaggcct cgcggtgcaa cagggccacg
    23881 gattccacgt gcgccgtctg agggaagaga tcgaagggcg tgacacgaga tagtttatat
    23941 ccctcttggc gcaaaacgcc cacgtctcgg gcaagggtgg cggggttgca ggacacgtag
    24001 accagggtct ttgaggccag ggcccctagg cgatgcaaca cctcttcttc gcaccccgtc
    24061 cggggaggat ccatcaccac cacgtcgggg cgctctccgg ggtctgccag ataggccttg
    24121 acgtcggcgc acacggtgcg gacctggcgg aaccccgccc tccccaagtt gtggcgcgag
    24181 ctttccaccg aggccaccca ggactccacc gccgtcacct cgccccccag gcgggccaga
    24241 aaggtcgtca ggcttcccac tcccgcatag agctccaagg tgcgggaggg gttggcggct
    24301 ttcaccagat cggccacgtg ggagaagagc ccctcggcct gagtagggtt gacctggaaa
    24361 aaggaggtca cgtcataggt gtaagaaaag tctccgaggc gctctgagag gaacgactct
    24421 ccccccacga tctccgtcgt tcctccccaa atggtattgc ccggggctcc gtggatatgc
    24481 accgccaggg tatatcgttt ggcggcggat cgcacgacgg gaaggatttt ttccctaagc
    24541 cagcgctctt gcggcgatga gaggggagcc cgcaccacca acacgcacgc cacgtgatcc
    24601 gtgcgaattc ccccccgtaa caccaggtgg cgcaggtctc cggagtgttt tttttcgtcg
    24661 taccccgtca gggcaccttt ttccagcagg gggcgcaggg agataaaaag gccttccagg
    24721 cgagggtcga ccaccggaca ttggcgaagg ggtacgatgc gatggcttcc ttcacggtag
    24781 aatcccagcc ggagcccccc cgaagggtga aagacggcgg ggaaggccgc cttgttccgg
    24841 tatccccaga ggacagagct tggacgacag gcttcaaacc ctgtctcggg aactcctccc
    24901 ccaaggcgtg tcagggcgtc ttgcaccagg gcgcccttaa gctctgtctg gaggatctcg
    24961 tccgcatgct ggatctgaca ccctccgcag gcgccaaacc agggacacgg gggagatact
    25021 cgcctaggat ggggccctaa gatttcctgg agatgaccca aggcgtactg gcgcttggct
    25081 tctaccactg ccactcggac ccgttctccc gggagtcctt ggggcacaaa gagcacgaag
    25141 ccttcgtcgg tgcgggcaat cccctctcct cggctgttaa tgcccgatat agttacttcc
    25201 gctgcttcgc cccgcgccaa aggtttcccc attcccatcc ctcttctcat tattggatta
    25261 gccatcccac accccggagt cctgggcctc cgtcggacga ttctagcatg gctcctctct
    25321 tgtgtgccga agattttccc atcgtcccct cgcgcggact ccgacgagga ggtttgagtc
    25381 gccagaaaga agtcaaaggg gtggtctcat gttacaatca acttacacct ccggacttcg
    25441 ccccacgccc cgccctaggt ccactccgaa ggttttgtga agacggggcg aagagccagg
    25501 gtggaccgca gccttcgagg tcctatgaag gactgccctc gcgattttca agctccggcg
    25561 tcccggggcg tcgaaagtcc taccgccggg cctcgaggcg aaagagcgat agaatcgtgc
    25621 gccagacgag gggcattctt tgcgtcctca ggaagctctc cccgagaggg cgtttcacca
    25681 taccactcca aacgaggtga gttccgtggc gacagacgaa ggagagagtt ccagacaccg
    25741 attatctctc gtgctggcgg atatcgtggc catgatcatt ttttccaccg ccctctgcat
    25801 ggtcatcgag atcttcatcg cggggctctc gttctggcaa tccgtaagtg cccgcgtggc
    25861 ggcgattcct gtaaatctcg tcaccggaag gccctacggg atcttccggg acaagctatt
    25921 ccagatcctc cacatcgaca gagtcaattc atggaaattg attttggggg ataccttggc
    25981 cttcgtggcc tttcaagttc ccctctacgt cgtcgtcttg ctcctggcgg aggcctcgtg
    26041 gcgacagatc gccatttcct ccgcctccat cacgttgatt ttttccctgg cagggcggcc
    26101 gtacggcatg ttcctcgatt tttgccgacg aatcgtctcg ggatttggga gttgatcgcc
    26161 cccactccga gaccgcctag gatccccagg ccgtctgtgg ggcggtctca gagaaagcgc
    26221 aaagcgcgga accggcagag gggtcccgcg ctttgcgctt ttaacaaatc cagactactt
    26281 caccaaggcg tacccttcgt caaaggcccg gccgttcagc tcctccgttc ccttgggtac
    26341 ccgggcgagg atggctttac ggatggattc gggcttcatg atgccctttt tctccaacgc
    26401 cccggcggcg gcccccaggg ctaccatgtt caccacgatc tctttcccga tcttctcccg
    26461 agccgtacga acgatgggaa ggtgaaacac atcggcgtcc acatcgggga tctcttcgat
    26521 gaagaagtca tcgatgatta ccacgctgcc gggcttgctg tctttgacgt attcgtcgca
    26581 ggacttttgg gtgaggatca cctgcaggtc cagggccgtg gccttggggt agtcgatgga
    26641 gcccttggaa ataactactt cagatttgga tgaccccccc cgggcctctg ggccgtaaga
    26701 ctggctctgg accgccgcca gcccttcgtg tagtaccgcc gcttctccca ggatcaccgc
    26761 tgccaaaatg accccctgac cgccggaccc tgccagacgg atttcgtaac gatcgctcat
    26821 ggccctaccc cctcacgcgc tcacagagct tccggtactc gtccgtgtac tcaggaatat
    26881 cccgattaac gaactctcca atgacgatct tacccttcag atcctccgga gacatggcat
    26941 cggccttggc tttgggcaca ctggcgtctt tgaaaaatac gaggttatcc acgggctgac
    27001 tgcgcttgtt tttacggcca aactgggtgt gacagtgggt catcacttcc accacagaaa
    27061 agcccttatg ctgaatggcc tttttgatgt actgttcggc catgacgggc tgggcaatgg
    27121 tggcccgggc tacgtaagag gcccccgccc cttcggccag cttacagatg tcaaagggag
    27181 gctcaatggc cccatagggg gcggtggtgg ctcgggctcc ctgaggggtt gtgggggacg
    27241 cttgacctcc ggtcatgccg tagatgttgt tattcatgat gatggcggtg atgccgatgt
    27301 ttcgtcggca ggcgtggatg aagtggttcc ccccgatggc ggaacagtcc ccgtcgccca
    27361 tgacgtcgat gacggtgagt tccggtttgg ccattttgat ccccgtggcg tacgccaagg
    27421 atcgcccgtg ggtggtgtgc accgtgcagg catcgatgta gcccggcatg cggctggagc
    27481 aacctattcc cgaagcgatg accgtttctt cgggtttttt ctccagatca accagggccc
    27541 ggagaagggc gtgcaggatg acgccgtgac cgcagccagg acaccagatg tgggggaaaa
    27601 atcgcgtacg gagccacttg gatacttgtt cgcggggcat agctacgcca cctcctcgat
    27661 cttcgcaaga atggcggaag gcttgaaaag ttccccattt accagggact gccccaggac
    27721 tcgggccctc cccgccacgg ccctctccac ttccagcacc atctggccac agttaagctc
    27781 cggcaccaga acgctatggg ccttagccgc caaggctcct acttccttgt ccgggaaggg
    27841 ccacagggtg atggggcgga agaacccggc cttgatgccc ttttcgcgag cttggcgcac
    27901 cgcccgcagg gcgcttcggg caggagaacc gtaggccacc accaaaacct tggcgtcctc
    27961 cacggcctcc gtttcgtact tgacgatatc gtcccggaaa cggtcgatct tgcgcatgag
    28021 gcggagcatc tttttttcga tgtctccgtg gttattcgtg gggaaccccc agtcgttgtg
    28081 ggtcaacccc gtgacgtgcc agcggtaacc atcgccgaaa gacgccatgg ggggaatgtc
    28141 gtccgcttcg tccgccttgt aggggacgaa ggcgtccggg gtggcatggg gacgcttgcg
    28201 atccaccagc atgtaagacc cgggctcggg tatcaccact ttttctcgca tatgccccac
    28261 cacttcatcg gacatgatga gaacgggctg gcggaagcgt tcggccatgt tgaaggcgtc
    28321 aatggcgagc tcgtagcatt cctgcaccga agagggggcg taggcgatgg taccatggtc
    28381 gccgtggctt ccccaccgag cttgcatcac atcctgttgg gagaccatgg tgggaagccc
    28441 cgtggaaggc ccccctctca tgacatccac caccaccagg ggtatttccg ccatgtaggc
    28501 caaccccaag ttctcctgct tcagggaaaa tcccggcccc gaggtccccg tcatggcttt
    28561 ataccccgcg atggaagcgc caatagcggc ggcgatgcct ccgatttcgt cttccatctg
    28621 gataaacttc ccccctaact tggggagctc ttcggcacag acctcggcta tttcggtgga
    28681 tggagtaatg ggataaccac caaaaaacct acagcccgcc gcaatggctc cctgggccag
    28741 ggccatgttt ccctgccaga actcaatttg aggcatgggg ttcacgctcc ttcaccgtga
    28801 tcgcaagatc gggacagatg ttatcgcact gacgacagcc gatacactcg gccactcggg
    28861 gggcttcgca cttggcccgg tcgttcagct ccagaacttt cttggggcat acggcgatgc
    28921 ataggccgca ccccttgcac caacccttgt taatgagcac atcaaacact ttgctcacga
    28981 aacgcaccat ccttccttaa aatggcataa gccaccacga tcctggaaga cctgaagatc
    29041 tcaggaggac acccttcgga agctattatg acaccagcgg cgcaaaatct caaggggtat
    29101 ttgcacaata cgcatcaaag caataattgt gcgaaaacta agaactattt tgtcaattcc
    29161 ttccgggcgt tccattgcgc cccccggcct cgtcccttcc cccaacgttt cactactcct
    29221 ggcttagatg ttttaaccac cgtgtggggc cgtataaaaa ggaggggtct ccccctcctt
    29281 taggactcat tacatccctt gcccagggct ttcctaaacc accgctggct cgtttatgag
    29341 ctccccggag gcaaaacgcc ggtaatggag catgccaatg gggaggtggg tctctccttt
    29401 gaggatgagc tccgccatga gaactcccgt ttcgggggcg agcatgaaac cgtgaccgga
    29461 aaagcccgta gacaggaaaa accccttcac ctcgtccgct tctcccagga tgggctggcg
    29521 gtcgggggtc atttcgtaaa ggccggacca ctggcgcacc aaacgaatgc ccttcgcccg
    29581 gggcaaaacc ttggtgatct tttgggccat gagttcggca aaggcccagg tgtgaccatt
    29641 cccaaagtcc tcgggatgtc cttcagggct gcacccgccg atgatagagc catgagggcg
    29701 ctgttggatg taaaagtttc ccgtaaagct catgagcatg gggggacata cgccgaactc
    29761 cacaggctcg gtgatgacga tctcgtggcg ctcggagtag ttgggaagct ccactcccgc
    29821 catcttggcg atgaactggg catagggccc cgcagcgttg atgaccaccg gagtggcgat
    29881 gtcgcccttg gtggtggaaa cacccaccac cttgccgccc tccaccttga ctcccgtgca
    29941 ctccgtaaac ttgtggaaga ccactccgag acgcctggcg gcctcctggt aggcgaaggt
    30001 ggtcaaaaag ggatcggcgt ggccatccct gtcgtgaaag gtgaacccca ccgcatcttc
    30061 ggcagcgagc cccgggcaaa tctcacaggc ctccttcagg gacaccacac gggttttgat
    30121 tcccatgctg ttctggaggg ccatgctgtg cttcagggtg gcaaactcgc tgtctttgta
    30181 ggccaccatg aggtagccgc actggtttag ccctacatcc atgcccagtt cgtcgctgag
    30241 ctgctcgaag atttccaggg aagcgatgcc caggcggcag ttcatctctg tgccccactg
    30301 ggcccggatc cccgcgccac aacgccccgt ggacccgttc cccacggtgt ccttctccag
    30361 aacgaccacg ttccgggctc ccatcttggc caggttgtag gcaatggcga ccccctggat
    30421 gccgccaccg atgacaacgt aatccgccgt atgggtccag gacatcactt gtcccctcct
    30481 ttggccaaag ctcctaattt gacgggtttt accgggggcc taatggtccc ctggggaatg
    30541 agcgcgtaag gcgtgcccgt gatctttgac acctctcgaa ggatgatctc ccggcatccc
    30601 cgcccctggc aagggcccat gcccacgcgg agaacccgct taagctcgtc gaacgtatcg
    30661 tatcctttgt gaatccactc tcggatttct cccaaggtga cctcttcgca gcggcaaatc
    30721 accatgtcgt cttctttttt cttcgccacc cggcccacct taatggttct cacgtcttgc
    30781 accttgtccc gggggaccac cacgtggacc accagggtcc tgtccttcca gggctcggtg
    30841 acgttcacga ccttgccccg gcaaatttct tgccccgtac ggttcatgca cgccacgtcc
    30901 tctccctttt ctggcactgg gaggatttcg taaggaagct tcatgagggc ttcgtcgggg
    30961 cctccatagg tgaggtccac cacgaggcat gccagcccgg ggcacttggc cacgcacatg
    31021 gcgcatcccg tgcacttgct gtagtcgatg cgaggggtgt cgttgatgtc gtcaaagggg
    31081 agcaccgccc ccgtgggaca gctcgtatga caggggttac atgggatcct ctgggggcat
    31141 tcgatgatca ctagaccatt ttttttactt tcccaaagat cctgcggggg aagtacggcc
    31201 cccgggcgct cttccgtgag cacaccgctg ttaaaaaatt tctctttgtc ggtcatggct
    31261 acgcccccca acattcgcag cagacttggc agagaccctc gcggatcttc tcgcccactt
    31321 ctccagcccg gagatcgctc agccgcttgt gatacatcgt ggagcgttct ttgagcacct
    31381 ccggagtccc gtagttgagg gccgccgccg ccgccaatcc cgctacgcgc ccctccacca
    31441 tggcggagga cgcctcttcg atgcccgccg catctccggc cacccagata tcggggtggg
    31501 acgtgcgcat gagggagtcg cgtttggcca cgtagccgca gagctgcggg acaaacttca
    31561 tctcacaccc tgcctgccag agcatctcgc aggtgggggt gagccccacc gccaagcaga
    31621 tgacgtcaca atccacccgt tggggatccc ccacgggctg gaacttggag tccacttcct
    31681 ggatgacggc ccccgtcacc acgtcggttc ccagggcttc cttgatggtg tggttgagga
    31741 ggatgggcac tcccatgcgg cgcaccttgg cggcgtgcac ccagtacccc ccgatcttgg
    31801 acagggcctc caggatggcc acgatctcca ctccggcctg catgagttgg tagctgacga
    31861 tgaggccgat gttgcccgct cccaccatga gcaccctctg tcccggagtg acgccgtaga
    31921 cgttcatgag ggtctggatg gcccccgcgc cgtagactcc aggcaggtcg ttgttgtgaa
    31981 agggaatgag gcgttcctgg gcaccggtgg ccatgacgat cttccgggga cggattcgca
    32041 catattcgtc ctcccccacc acataggaca ccacgccgtc ttctttgtag tatcccgtag
    32101 ccgtgcagtt ggaaaggact ttaatttttc cctgagactt gtagtcctcc agctcccgga
    32161 acaagaggtc ggcgatcagg agtcctcggg tccccgcatg ctcctccttg gagccaaaga
    32221 atttgtgggt ctgctttatg agctgccctc cgagcttgag gtcgctgtcc aaaagcgtca
    32281 cttcagcccc gagggacgcc gcctccaagg ccgccgaaag ccccgcaggg ccgccgccga
    32341 tgaccagaat ttccgtctct cgcatggtct tcgatcctcc ctaggcgccc acggggggca
    32401 cgaggccctt tccggtctgg gtttccaccc tcatcccctc ggtgagggcc gtgacgcagg
    32461 tgcgaacgtt ggggactccg tccaccacca tgaagcagga cgagcactta ccgatggcgc
    32521 agaaaaaacc tcggggacgc ttcatttcgg gagttacccg gtagatgcgc acgccgttgg
    32581 cgtggagcgc cgccgcgatg ggctcgcctt cgaacccctc catctcctgg ccgtcgaaga
    32641 aaaattgcac ctttcggccg cgctgaaact ccagaatcgg gtgtttctcg atcaggttca
    32701 ctgccacccc tccttgagat agaaatgaag agtcttgcgg aaaaaccggc gtgaagagct
    32761 ccgcagactc cccagggttc ccccgtgcgc caccgccgcc cccttcggca tccctcccca
    32821 tcgtgatctc cggtgccgct acaccacgct ccattgtcat gtaccatatc agatatacca
    32881 cgatcgccgg aggcttgcaa caaggaaacc taattcacgt cgtgttttct caaataggag
    32941 gctcgaacgc tttgaggcga catcgcatca cccaggcgct tctctcctaa gagggccact
    33001 atagcggcgc tgatacgagt ttctaccctt cgtactccgg cggaatctgt ccagggggcc
    33061 caacggtcta ttccctctcc caccacgatc tgaggagatg gaagcaaacc caaaacatta
    33121 tttagttcaa tatcttccac catttggggc gaaaggagtt cttccccctc ggaaagatcg
    33181 taggccccta catagctcgc cccgtggcgt gcccagatca tgggtaccac cgtgtgggtc
    33241 gcccccacag cggaagatgc cagggcttcc aaggtgccca cggggaccac cggaagaccc
    33301 agagcttccg ccagggccgt cccataggcc agtcccactc gaacccctgt gaaatacccc
    33361 ggtcccaccg tcagggcaat ggcgtcaaga tcggcgagat ggagctccag ggcccgcagc
    33421 agcccttcct gtacgacggg aaggagggcc ccctggcgcc gatccagatc taggtggata
    33481 tcccccaaca cggccccgtt ccgtacacac cccacgctgg ttttccgtag gcaacaatcg
    33541 atcccgagaa ttacacccat tttgcccatc cctccaccat aggtagaaaa cttccgccgt
    33601 ttcaccccaa aaggcctcgt cgtctccccc ggaaattatt atcgtaaatt ttggcttagt
    33661 ttaataattc gcccaatctc ttgtctttca aggagtaagc tggggagcgc gaagctcatg
    33721 ccttgaaaaa aggcttttcc ctagcttccg aaaggatagc ttttccccgg aggggtcgca
    33781 agggggagtt ttttttgcct ccccttctgg tacaatgtgg tggtgaaaaa gggatcgtag
    33841 tcatgacggg gggactgggg agtggccatg gacgtggctt tgcagcttct tggcggcgtg
    33901 ggactatttc tttacggcat caaactcatg ggggacgcgc tacagtcctt ggcgggggat
    33961 cgcatgcgcc gcctggtggg atccctcacc cgcacccccg tgaggggagt gctcataggc
    34021 acctttgtca ccatgctcat tcaaagcagc agcgccacca ccgtcatgac cgtgagcttt
    34081 gtccacgtgg gactcatgac gctgcagcag gccgtggggg tcatcatggg ggccaacgta
    34141 ggcaccacca tgaccgccca gatcattgcc ttcaagctga atgcctatgc cctgcccctc
    34201 atcggcatag gcatgtttct ggccctcttc gggaaatcaa agaagcaacg ctacatcggt
    34261 aacggaatag tgggtttcgg cctgctcttc ctcggcatga agaccatgga agactccatg
    34321 tccttcctgc gggggcgcca ggaccttttt ctctccttca gctccaaccc catgctcggg
    34381 gtgttggcgg gaacagtcgt aaccatggtg gtgcaatcca gcgccgccac catcgggttg
    34441 accattgcca tggccgccca ggggctgttt ggcctggacg tggcccttcc catcgtgctg
    34501 ggggataaca ttggcaccac catcaccgct gttttagctt ccttcgggtc gaaccgatcc
    34561 gccaagcagg cggcggcggc tcacgtgatg ttcaacatca taggtgtgac gtgggccctc
    34621 ctcacgttgc ccctcttcca atccattgtg atggtcaccg ccaaagacgt ttcccatcag
    34681 ctcgccaatg cccacaccct cttcaacatc ctcaacacct tgtttttcct tcccttcacc
    34741 gccccctttg cccgactgat ccagtggata ctgccctctt ccgagagcgc caccccttcg
    34801 gggccccgct tcctcgatgc caaacttctc aaggcatctc ccgccgccgc cgtggatgcg
    34861 gtgcgccagg agattgtcca catggggtcg ctggcccaat tgatgttgaa ggacgtgttg
    34921 caggctttct tggaaaatga ccaccgcatg atatctcagg tcaacgagac ggagaaggcg
    34981 gtgaacgagc tcaaccatgc catctccggc tacgcctcgg agctgtggca agaggggctt
    35041 tccgacgaac tctccgtggc cctatcctct tatgtcaacg gcgtgggaga cattgagcgc
    35101 atgggagatc acgcccagaa cctgattgag ctcttcgagt acaaggaaga acacgggttg
    35161 cagttttcca aagagggtcg cgaagagcta caagacatgt ttgccacggt gacccttgcc
    35221 gtgaagttgt gtctggaggc catggcggag gagaacgtgg ccaaggcccg ggaagtcatc
    35281 gaccacctcg aagaggagat cgaccgcaag gagaaaaccc tgcgccgcca gcacatcatc
    35341 cgcctgaatc gaggtatttg ccagcccagc gcgggggtgg tgttcatcga catcctgagc
    35401 aacctggagc gcatcggaga ccatgcccac aacgtagcct atatcgtctt cgacatcgcc
    35461 cgactccatc acgacgtgcc tgaagcttaa tcgcggggca ctcatagggc tctatcattg
    35521 ccccacctcc ggagctccgc cccccaggcg aggctccgga gtcgtttttc ccaccgcagc
    35581 gctggcaaag ctccccatgc cgatgggcac gggtgatcgg gaaaacgcga tgggagggac
    35641 tcaaagttat gatccatcca agacgccctc cctggggcga tacggcgaac cggagccctt
    35701 gggagatcgc cgaggcaagg ccttggcccc tctcccccac tcccatggaa gggggagagg
    35761 ggagaaaggg gcccgaaggg tgatggggca agaacactgt gtcaacctat aaaaaaaaat
    35821 agttgacaag aatttacccc ggagctatgc tcgtccctgc gataaaactc tcccgaggag
    35881 gcgctcattg tcgtgaaaga aaaccccctg gatttctccc ccatcccccg gggacaaaag
    35941 ggcctttctc cccaccacat ggccctggtg gccctttttg ccgtcctcac cgccgtgggg
    36001 gcctttatcc gcgttcccct tccctttgtg cccttcacct tgcagttttt tttctgcgcc
    36061 ctagggggac tgttactggg atctcgcctg gggtcttacg cccaagtggt ttacatggcc
    36121 atgggactcc tggggctgcc agtattttcc ggcggagggg ggatccagtc cctcctccgc
    36181 ccttctttcg gctacatcgt gggattcgtg gcggcggcgt gggtcatcgg gcgcctgggg
    36241 gaacgggaaa aactcccctg gaccttttgg agcgccacca gggcgacctt ggccgggctt
    36301 ggggtactct atctcgtagg ggtgccttgg ttgtgggcgg tctactcctg gtatttgaat
    36361 gaccccaaac ccctatcctg ggccgtgacc tatggatttc tcatcttctt ggggggagat
    36421 ctcgtcaaaa cggcgttggc cgccttcgtg gcctgccgca tgggagtcct acgaaaaggg
    36481 agggggtgaa ggaaccaatc ctcccaccgc caccaggggt aagaaaaaaa cggttcctcg
    36541 cctgcaaagg cgaggaaccg ggatatcctg gagcggaaaa cgggactcga acccgcgacc
    36601 ctcagcttgg gaagctgatg ctctaccaac tgagcta
//
LOCUS       DAOOER010000014        36349 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_1279_length_36349_cov_8.637213, whole genome shotgun sequence.
ACCESSION   DAOOER010000014 DAOOER010000000
VERSION     DAOOER010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 36349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36349
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1279_length_36349_cov_8.637213"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PL162_03360"
     CDS             complement(<1..292)
                     /locus_tag="PL162_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91100.1"
                     /translation="MYRAKKIDTPDRKLFYRTLERLLESLLEGEPDAGANLANAAALL
                     GHQMERINWAGFYLFREGELVLGPFWGKPACSRIPLGKGVCGTAAAERRTVLV"
     gene            503..1741
                     /locus_tag="PL162_03365"
     CDS             503..1741
                     /locus_tag="PL162_03365"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQF91101.1"
                     /translation="MGFRIMVVMFHTWGFLYVFWRLGALFPSGSRSRKLLHAGGVLLW
                     SALPLGLSGERFLSPSVALFLLDLGFYVMPVLLHAAMVALLIDLYHVLSWMFPLPKMA
                     LLQRRRLWAWGGLLVALVVTGGYLNARWLRQHDMTVTLPWFAPSPPPAPLRLAVVTDL
                     HAGAMVHGGHLRRIVDLIVATSPDAVLLVGDILDGHIGQARKAELAEELRRIPAPLGV
                     FAVLGNHEFYAGIDNARAYLREGHVRVLEDEAVALGNRLLLVGRIDLQGRIFGHRRRP
                     LAKLLPSSRDEGGLPAVVLDHAPHFDEAATAGVALQFSGHTHHGQLFPFNLLVRRLFG
                     FSSGWHHQRKTLFYVSPGSGTWGPPVRTTARPEVVLCTVRFSSATDVAVLEPEARTGS
                     AGSSGAWGAPPAVSPAPSPE"
     gene            complement(1728..2909)
                     /locus_tag="PL162_03370"
     CDS             complement(1728..2909)
                     /locus_tag="PL162_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HQF91102.1"
                     /translation="MLVREKTTQAVGILQETKIDCWLTFVRETAEQCDPVLELIAGCD
                     VVGQAAFLLAADGRKVAVVASFDRFDVEALGVYDRVETYTTSLAEPLRRVLEEMDPQS
                     IGLNYSPWSSAADGLTLGMYQRLGALLEGTPYPGRFVSAEPVISRLRGRKTPEELRRI
                     RGAIGETLELFDLVTRSVRPGWTMRGVADFLHEEMARRGLGFAWSPAGDPAVDSGPHV
                     EAGHGAPPELPILPGHALHLDFGVRREGYSSDLQRMWYVKPPEEDRIPPELERALAVI
                     QGGIRSAAAILRPGVAGWEVDRVARQALEEAGYPEFQHALGHQVGTRAHDGGALLGPR
                     WERYGDLVTRPVEEGQVYTLEFGVRTSRGYVGQEEMVRVGAQGCEFLSPLQDGFWIIP
                     G"
     gene            3075..3863
                     /locus_tag="PL162_03375"
     CDS             3075..3863
                     /locus_tag="PL162_03375"
                     /inference="COORDINATES: protein motif:HMM:NF014609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HQF91103.1"
                     /translation="MELFTVDAFTDKPFTGNPAGVCFLDGPRDTAWMGAVAREMNLSE
                     TAFLLREGTNWRLRWFTPRVEVDLCGHATLATAHVLREKGVLRPGETVRFHTRSGELS
                     ASAEEGRIVLDFPAKEIEACPPPEGLLEALEISDPLFVGKNIFDYLVEVGSDAQVRSL
                     APDFPRLAHLEARGTMVTARSSESGFDCVSRFFAPRVGVPEDPVTGSAHCALGPYWGA
                     KLGKTRISAFQASERGGAMEVVLVLPRVLLKGQAVTVLRGHLEA"
     gene            4115..4630
                     /locus_tag="PL162_03380"
     CDS             4115..4630
                     /locus_tag="PL162_03380"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HQF91104.1"
                     /translation="MDTRGRFVRTIFDAVLLVFALGWSAGAQTSEDTTLSPDAIQAAG
                     KALLAGTSGDAPVERVLVYYLHGTARCPTCQNIEKFTYEAVTANFGAELASGAMEWHS
                     LDVMTDEHRHFVEDFQLVAQSVVLVRMAGDRMEAWKNLEQVWPLAHQKEAFLAYVTEE
                     LRAFLEGDTPR"
     gene            complement(4722..5891)
                     /locus_tag="PL162_03385"
     CDS             complement(4722..5891)
                     /locus_tag="PL162_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HQF91105.1"
                     /translation="MNDEVKKFFSLAAMFLVFYFLPVDSPRVQGAAIEALAMLHEYAR
                     EHVLFCLVPAFFIAGAISVFVSQQSVLRYLGAKASRGVAYTVAAISGTVLAVCSCTVL
                     PIFAGIYKRGAGLGPASAFLYSGPAINVLAIVMTARVLGMEMGIARAVGAVGFSVVIG
                     VLMAFLFRHEEQERQQAFADMPVPEADRPLWQTVFFMASMVVFLVFANWAAPKIPLGV
                     WAAVYQVKWWIAGAALISTVLAARLWFTPDARAAWYGSTGEYALQVVPLLFGGVLAAG
                     FLLGRPGHEALIPSQWVAALVGGNSLLANLFSSIVAAFMYFATLTEVPILQGLLGAGM
                     GKGPALALLLAGPALSLPSMLVIRSILGTKKTVVYVGLVILLSSGAGMLFGALFA"
     gene            complement(6156..6518)
                     /locus_tag="PL162_03390"
     CDS             complement(6156..6518)
                     /locus_tag="PL162_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQF91106.1"
                     /translation="MTENLYEPSCGASPRQGEYQQAKSNILKAVAHPIRLSIVEMLGE
                     GERCACKICDAFSCDRTTVSKHLAVLKSFGVIEDRKEGLFVFYRLRMRCLLPFLACLE
                     RVVCGAPCGCSGAEDDHV"
     gene            7003..8058
                     /locus_tag="PL162_03395"
     CDS             7003..8058
                     /locus_tag="PL162_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91107.1"
                     /translation="MQVTRKDLDDMESQGILTPGQGELLWRALREKGAGRPRFDLAHV
                     LYYLGALVVMGAMTYFMTLGWASFGGGGLASVAAAYMLLFAMAGRTYFAAPQTRVAGG
                     LLWTLALAMVPLLVYGLQESLGLWPQEFPGAYRDFYVWVRGGWFWMEVATLGVGALIL
                     RRVSFPFLLMPISLALYFLSMDLVPLIYGQEFTWDQRLWFSVWFGLALCGGAYAVDLK
                     RLRHGGEDFAPWLYLFGATALWGGLSLLDSGSELRRLGYATINVLLIFFSLLVDRGVF
                     LVYGSLGFFGYLSYLSYTVFADSLFFPFVLSALGVVLILAGVQYRKYEDHIKKGLRRL
                     LPPGMAPWIPVAYREDE"
     gene            complement(8283..8588)
                     /locus_tag="PL162_03400"
     CDS             complement(8283..8588)
                     /locus_tag="PL162_03400"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HQF91108.1"
                     /translation="MKDEGLLALILGMALVTYLPRLAPFVLLHRWSLPPGVRHVLEQI
                     PAAALGALLIPDALGALPGHPGVAAVGLGVAVALSMAGGNLILTVMGAVGAVYVLLR"
     gene            complement(8585..9280)
                     /locus_tag="PL162_03405"
     CDS             complement(8585..9280)
                     /locus_tag="PL162_03405"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HQF91109.1"
                     /translation="MAHGVKDFWRGFRCGIPIIVGYVPIALAFGVLAKTGGIPLWGAA
                     LFSGVVFAGASQFMALALWGGGAGMGEIILATFLVNFRHFLMSAALAARCPGLRGALR
                     PLLAFGVTDEVFSLASLRETPPSSAEVLGLEAAAYGSWVGGTMLGFLLGEFLGETLRA
                     SLGIAIYAMFVGLLVPAVRRLPRRGAVALLAGGFHLALVHLPGVSSSTALVGGMVLGA
                     LVGSWGTPKEEGA"
     gene            complement(9390..10472)
                     /locus_tag="PL162_03410"
     CDS             complement(9390..10472)
                     /locus_tag="PL162_03410"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HQF91110.1"
                     /translation="MIPITKPTLVIDEARCRENIAFMAQKARDRGVDFRPHFKTHQSL
                     AVGRWFKHVGVDKIAVSSLEMAQYFSEEWSDITVAFPVNVLERDLINALAKRIRLNLL
                     VESLETVDHLKKDLRHPVGVFIKIDVGYGRTGLTPGDREGMEHLMEALASGEAMTFRG
                     FLSHAGHAYACRGAREVVAVHEEARRALGELKEAYRPIHSDIIASLGDTPSCVLAENF
                     EGIDEIRPGNFVFYDLMQVQIGTVPVDRIAMAVACPVVAVHEDRGELVIYGGGVHLSK
                     EFLEEDGRRVYGRIARPEGDGWGPALEGAYVRSLSQEHGVVVAPRDFLSRCHVGDLVM
                     VLPVHACMAASALRCYVNNNEILEKF"
     gene            complement(10624..11772)
                     /locus_tag="PL162_03415"
     CDS             complement(10624..11772)
                     /locus_tag="PL162_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQF91111.1"
                     /translation="MKLWRCCALVLAGAVLLGAGVAGAEETLKIGVLAPLTGFAAADG
                     LSVKNALELATEQVNAKGGVLGKKLELVIYDDAADPKESVTLAQKLIEQDQVVAVVGG
                     SYSMPTRVTTPIFNEEEVPMVAAYAIHPDVTEGEFTFRNGFLGKVEGGAAAYVVDTML
                     KGKKVALLYADNDFGKTLAQGFKDYVAAKGTPQVIFEQAYPAKEKDFSPYLSKIKEGA
                     PDVLVAFGYYFQTGPVVKQAREMGITAAILGEEGADSPKLFEIAGPAAEGFVLVTNLN
                     RDDPRPVVQEFLKSFREKYAMEPDMVGASAYDALMIIVDAITRSGGTDRKAVRDAIAA
                     VKDYDGLTGIIQGFTEAGEVVKPVQVQVVKDGKFTYMGVVDDPQLIRP"
     gene            complement(11844..12551)
                     /locus_tag="PL162_03420"
     CDS             complement(11844..12551)
                     /locus_tag="PL162_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQF91112.1"
                     /translation="MLKVENLRVSYGDIEAVHGVSLEVGPGELVSVIGANGAGKTSLL
                     GALMGWVPASGSIVFDGEEISSLAPHHRARRGIRIVPERARIFPQLSVRENLRAGVYG
                     LKIGEESFRRRLDELYALFPILQERGGQLASTLSGGEQQQLAIARALVAEPRLLLVDE
                     VSMGLMPKLVDEVFAVLRRLNREKGLPVLLVEQNARASLAISDRAYVLETGNLVLSGT
                     AEELEHDARVREAYLGL"
     gene            complement(12544..13341)
                     /locus_tag="PL162_03425"
     CDS             complement(12544..13341)
                     /locus_tag="PL162_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQF91113.1"
                     /translation="MTDVERKDHASSREPLLSVRGLSKRFGGLQALEDVSFDLHPGEI
                     LGLLGPNGAGKTTCFNMISGMFSPTAGRIFFDGTDTTGVSPAEMARRGVGRTFQIVRP
                     FPSLSVLENVSVSLGLRRYGSLGCLLGRWGTRSVREEAREILASVGLADLASRRAGLL
                     PLGNLRRLELARALALRPKLLLLDETFSGLRAEEIRQMELHVRGVRDAGVGVLLIEHN
                     MKVAMALSERIVVLDHGRKLAEGTGAEVAADPRVVEAYLGKGASGHA"
     gene            complement(13338..14222)
                     /locus_tag="PL162_03430"
     CDS             complement(13338..14222)
                     /locus_tag="PL162_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQF91114.1"
                     /translation="MSYLVTLGIFISIHAIVACGLNVIVGYAGQISLGHAAFFGIGAY
                     AAALLATRGDLSFWAALPLVLLITATAGLLLGLPSLRVREDFLAITTIGINFIVESLF
                     LYVSFFGGALGIGNIPRIVLFGERLKGPTFLALCLFFLALALLISWGFTRSWAGLACF
                     ALRDDEGAAASMGVSPVRFKLLAFVLGTTFAGLGGALYAHHMRFVSASDFGFPVSVML
                     LSMVVLGGMGSLWGPVLGALILGVLPEIFRPLTEYRLLLYATLLLLMIRFQPGGLLGP
                     GSLARKLFSFRRKGGEVS"
     gene            complement(14238..15101)
                     /locus_tag="PL162_03435"
     CDS             complement(14238..15101)
                     /locus_tag="PL162_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQF91115.1"
                     /translation="MHLFLEQCASGVAMGSIYALITLGLALVYGILRILHVAHAAIYA
                     VGAYLALGIFSLSGSLVLAALGAMGGCALLGVAMERWIYTPLLSFPPFVPLISSIAML
                     LGMEEICRLVAGPYILPFAVELPVPDITLGGVRFSGPLLSVYGVTALVLGGLWYLTTR
                     TELGLAMRATAQDRNMAAACGIPVHRVVAVTFALGSAVAAVAGMLVGIYYNQVYPTMG
                     AVPAYKSLALIVVGGLGSVPGAVGASLLLGVAETLLIGYADIPLPRDALAFIAMVAVL
                     MWRPQGLFGHR"
     gene            complement(15229..17589)
                     /locus_tag="PL162_03440"
     CDS             complement(15229..17589)
                     /locus_tag="PL162_03440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQF91116.1"
                     /translation="MGSPGNRKKIWKGSARPGTPWGHLRAVVDGIDQGYVLLDPAGRF
                     VEVNEAFCRITGLRREAVTGHTVEHFVSPEHAGSWKSAVAVALTLSAGGSFDAVLPLG
                     GGVPRPVRVEYRALSGEPFPRKGWRWVLITDLSGRLPSERGDGGREDRRRRALEGVSV
                     GTWEWHLPGGHVVFDEEFVHMLDLLPGALLSDLAAAKALVWDEDLPGLTRRLRRILGG
                     RSDRFDLELRLRHSRGSPVWVLGRGRVTERDSKGRPLRVGGSVVDVSRRKRAEGALEE
                     SMRSFRAILDGFTDRIFHLDGHLRILWANEAVRRPFPGCVGERCHEALFSSPKPCEGC
                     PCVEALRSGKLERRVFHDAVGGMGEGYWEVSGIPMKDLRGEVTELVSVGRDITEQSEL
                     ERGLREARDRAEEASRAKGAFLANMSHELRTPMNAIIGMTDLLLETPLTREQRDLLSS
                     VRSSSGSLMEIISDLLDFSRIEAGRFELSQELFSVRDLCVQVVWTLDVAALRKGLILE
                     HREEGSVPDRVRGDPLRLRQVLVNLIGNALKFTDRGRITFTVRGREREGGKAEIFFEV
                     RDTGVGISEEKKPLIFRPFFQADLSSRKRFQGTGLGLAISHQIVDAMGGTLAFESSEG
                     HGSTFFFSVVFPLVTPPQVVAPSLPLAPSRRILVVEDDPMQALILRRLLERGGWKADV
                     VATGEDAKRCFREESYDAVILEGHLSDGSGAEVARGLREIEEGGFRRPFLVLGQEPEG
                     EVDAGVEKPIDPEALVGLLGKLVGSLRGGGEEGFGPGGNPFTAKSD"
     gene            complement(17592..20093)
                     /locus_tag="PL162_03445"
     CDS             complement(17592..20093)
                     /locus_tag="PL162_03445"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HQF91117.1"
                     /translation="MTEREQYLKLFLDEALEVLESFHGALLKLDAAPGDLMAVGEAFR
                     AAHTIKGMSGAMGFSNMQRLTHAAEDLLQDIRDGKVEIGEQLLGMLFVSHDLLRNALD
                     EVARAGDDTGVAVGEVLERLHALIHSHHGGQARRRGGPKAVSLPPNEKDTLEEAAKRG
                     LQPFRLEVALVDECPLRVVRAFMVLRLLGSLGEVFRSVPRMEDLQKDPDAVESAAGSG
                     SFRILVFLLSSKPRELLEARVRDVVDVKDVLLEDLREKAAASPPVRLEREGASPGSKG
                     AVVLDGAFLENALEEMLGYVLLLRERFSRMEGGDGGALEALFRGYRSLRGFSRLAGQD
                     QIRILASAGERLVRDRLERGIPLEPEEMAALRRSLDPLEALCRHATSAQDPATEKGVA
                     ALAALLEGMHAPVPPQGVAREAPREGGGASREADHATDRGDALAAASIRVDRGKVDKL
                     LNLAGELVSLKNAFLHVARRLGEVSPHFANELKARGVNLERLTAELQAAVMAMRMAPV
                     ETLFNRFRRVVRDLARDVGKEVDLAVEGGETELDRAVLDKLTDPLTHMVRNSMDHGLE
                     PSQERVAGGKTPRGTVTLRAYTQGGYAFIDVRDDGRGIDPARLRVKAVERGIFSPEEA
                     ARRSDHDILEVIFLPGFSTAEKVTNLSGRGVGMDAVLSSLENVGVRITVASEVGRGSC
                     FTLRIPLSLSSIHGLRVRIGEGTYVVPLESVRETLKVPRSALQAYADFVLVRVRGAAL
                     PLLPAEAGLFGHAVDLAQKSFEFDLIPLVIVGSGRNQVALVVDDLLEEEEFLVKPLPG
                     SFRFGGATGATILGDGSLALILDPGVLVDQARESS"
     gene            complement(20093..20488)
                     /locus_tag="PL162_03450"
     CDS             complement(20093..20488)
                     /locus_tag="PL162_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQF91118.1"
                     /translation="MKSLVVEDEFLNRKVLSLLLDPYGPVDVAVSGAEAREALRIALE
                     EGEPYDLICLDIGLPDADGQQLLREIREAEESRGIHPHEAARVFMVTSQGDIENVRRA
                     FANECDGYLVKPVDRDSFETQLREVGLVP"
     gene            complement(20485..21549)
                     /gene="cheB"
                     /locus_tag="PL162_03455"
     CDS             complement(20485..21549)
                     /gene="cheB"
                     /locus_tag="PL162_03455"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HQF91119.1"
                     /translation="MIPLESRHSVRVLVADDSALMRRTFCRLLEEEPGLEVVGIARDG
                     LEAVEKARDLRPDVVTMDLNMPRMDGLSALQLVLEEELAQVLVVSSLSREGALVTFEA
                     LELGAFDCVEKPGGTISRSLETVRDELVSKIRAAAASRPRRVGPRTPRSSPAPKRDAP
                     KGGAPFSAVALGISTGGPRTIYDVLPKLPGDLNAAVFVVQHMPPHFTGMYAERLDASC
                     ALRVVEADRGMAVTPGVVYVGRGGYQLCVDRRGDEIKLSLPSQPRSTFIPSVNVMMHS
                     VREAFGPRTVGVLMTGMGDDGAEGMGAIRHSGGYGIVESEETAVVFGMPAEALRRGGA
                     DVALPSYRIAAKIVEVVKKL"
     gene            complement(21546..23078)
                     /locus_tag="PL162_03460"
     CDS             complement(21546..23078)
                     /locus_tag="PL162_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HQF91120.1"
                     /translation="MAGAGQYVTFSLGEVVYAVPVAAVREIVRLPAVTRLPRSPECVE
                     GLANLRGEVLPVLDLRTRLGLPRSSEEGRKVVVFDFDGLGVGVAVDRTSQVLRVEEGQ
                     VERSPGGEGSSELLAGVIRFREEVFMVINPQELLTGVTSWERRDRRQGSEAGSLAAEE
                     GPREPRVQVVTFEVGGQEYAFPIVEVREIVRYTPPTPFPETPPWVKGVMNLRGHTLPV
                     VDLRMRLGMAETAVDEFTKVVVLGSGSASTGFVVDRIHEVLRVTEGAMTPPPPLVARA
                     NATVGGIIEEKDRITVLLNPEALLEGRGEPTGGKESPENALPGGIDQGTHVPLVLFTV
                     GGQPYGVPIEQIREICRMPSVTPIPRSPHFVKGVMNLRGEVLPVVHLGRKLEIADSGG
                     EGDGDPRVLVFENAGVTVGFVVDTVVGVERFDRENLLLPPRGGEKGSREAVHRVARRG
                     DGSVVLVLDLGTVLSPRESSRVGALADRASAEEGVNEAPAPSTEGAPEPLKPGRRLRK
                     AR"
     gene            complement(23082..25073)
                     /locus_tag="PL162_03465"
     CDS             complement(23082..25073)
                     /locus_tag="PL162_03465"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQF91121.1"
                     /translation="MAKRLEVPESLKHLEENREGAKDRRSTGLVPREEGSDGDLAAKR
                     DLARRRAQERVRARTFAKRQAMAERISAAAEELLAGVEEASSSAEQLDRAMQQIAAGA
                     HQAEGAAAEAHGRTVMVEKSAGAFVDLGHEAHHKMTAIRGITGDATKNIGEANAAIQE
                     ATARNKASAETVRRLHDQSRQVEDIVKTVVMIADQTNLLALNAAIEAARAGEHGSGFA
                     VVADEVRNLAEVSEKSAREIRDVVGGINVLVSTVVEDIEGVVGASDAQVARGDEAGET
                     LAFLDGEALGALKGVEASLTIIADIAEKARAFLEDARVVTESSQISTTLAGECAKAVR
                     EQSRALQEIASATQELVDLADELKNSTQTDKSSERVAAAAEQLSSNLQESSASARQVA
                     GSMDRLATSATAVAQAAESATRLSVDIATTTATMQGTVARRIALIHTCHDRAQELRGK
                     IDDIIKGVQKVFRMLQGTAVNLSDLENMGRRIDKTVATIDKVTIQTNMLAVNGFIEAA
                     RAGDFGRGFSVVAGDIRNLAGESGENADRIKDLVFAMQRQIGAVLSDVSDASRQSGAE
                     NAKGAISLRLIEDLVRLFQETSKLYERIADVVNSIEECTRQAHASVENISAASQQGAA
                     AVEEAAKAAAEQARGMEELSRAVEEIAIIADELQSEAGV"
     gene            complement(25099..26592)
                     /locus_tag="PL162_03470"
     CDS             complement(25099..26592)
                     /locus_tag="PL162_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HQF91122.1"
                     /translation="MIVGIDFGTTNSSVAYLTPRGPELVVNERGERMTPSVVCFRSPH
                     EALVGELARSGGLLRPDQTIARVKRRMGNPAPYRVFGRELSPAGVGGLVFAKLRRYAE
                     DYLKETVTQAVVTVPAYFDDAQRHDVLEAAARGGIHVVRLLNEPTAAALAYRPAQVEE
                     GTFIVLDLGGGTFDITVLHSSKGLHQVRATGGSTELGGADFDDLLGEWVLEEVRRGRG
                     VDLGADPVARASVMRGVERVKIDLSSVEESMLLLPYIASGPEGPIHVQTEISRKTFER
                     LAEGLFAKIRGLVQSVLEESHIAPEGIDGVIYAGGSSRMPRFRREMEELFPRASVRGE
                     LNPDEVVALGAAVCAGIMEGRLENVELRDVLPHALGVRDDRGEFVPLVDRGTVYPVTA
                     VRLFTNAREGQKEVTVEVLQRRDDRLVPLGVLEFCAPTAWAQGEAELAVTFAVDASGV
                     LAVAAEDEATGKTGEMTIPSGVLPGAPWEEWEEGDRGELFLGDVTVL"
     gene            complement(26589..26888)
                     /locus_tag="PL162_03475"
     CDS             complement(26589..26888)
                     /locus_tag="PL162_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91123.1"
                     /translation="MTLASSLESLLGDSMESVYREAGRLEEQGKFLQAFHLFLRVAEA
                     SPPPLAARALNNAAVILSDHGFEEDAREMLRLSLRQDGQCAEARENLACLGDGPS"
     gene            complement(26885..27649)
                     /locus_tag="PL162_03480"
     CDS             complement(26885..27649)
                     /locus_tag="PL162_03480"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HQF91124.1"
                     /translation="MARVLAVANHKGGCGKTTLAVHLASWLSRKGKTLLVDLDPQGHA
                     GLCLGVRGEGARGEPGGTAKELRRFLEGGRDPRLRSLGDLDLLASDEELDGLEREEAG
                     GDALLRDYLSMCLRGYAFIVVDSPPAAGRLGRMALVAADVLVVPVRTDFLSLAGVARM
                     MASFYKVSATANPVLRLGGVAPTMADLRTRGAREAVEELRRNFGEEMMLPPLRLDVKF
                     SQAAAAGRPIMAFAPRSRGAQDVDDLCRILWRRIRP"
     gene            complement(27652..28476)
                     /locus_tag="PL162_03485"
     CDS             complement(27652..28476)
                     /locus_tag="PL162_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91125.1"
                     /translation="MIPRSDQAEFARMVEDFLDDFDGGAPEDLLLESQAPQALRRFLH
                     SAKAGFALLLAVAPSQPLAEVLHKGEILAHDLEGTERSLKDLEGDRKKRLERGMAALR
                     RLVEELRCLSRSPDSRGTLLPEGEGSPLPGPERPSEGRHPSPGEGSVAALLVRVEGIP
                     CLLSLEDVEAVGRLAPASKGWVLFRGEALPFFPARQVWSFPRSALRSALRPSDASVVV
                     RSPGGRAVLGVDRWEKRGEFPTRLVPEGFRFGNVRRVAFLEGGEVALVLTMPERWP"
     gene            complement(28473..29444)
                     /locus_tag="PL162_03490"
     CDS             complement(28473..29444)
                     /locus_tag="PL162_03490"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HQF91126.1"
                     /translation="MGEKDPREGRGYPSGERISGDFPRPPGPRGGEPGVGGPRKPPED
                     FRCFEEIRSFVHRHTGIFLGDQKRMQVFRHVRERMGVLGIDREERFINHLRFFDPQRK
                     EFQVLINRVTVNETYFFRDFPQLQSFAEDCLPELVAGCRRGGRRGLRVLSAGCATGEE
                     PYTLAIILREMLEEEGLPFEVVAVDINDSVLERARRGRYGRRAVKDVPKAYLDRHFHR
                     VGEHFVLRDETKRPVSFFLGNLADPQDMGQLGPDFDVVFCRNVLIYFDDEARQRMVEQ
                     LFHHLRPGGYIFLGHAEPVSRMSSRFRVKRTRGMVLYQKPLFGRGAT"
     gene            complement(29461..31557)
                     /locus_tag="PL162_03495"
     CDS             complement(29461..31557)
                     /locus_tag="PL162_03495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQF91127.1"
                     /translation="MTYAGADDQCDRLRRWLDALPAAIFLKDGAGRYVECNEAFAAFA
                     GCPRSELLGKTDWGIFPQELASEMVWQDMALRNDGGVRQFEFSAAQTPGDPPGLLSEA
                     EPSPEESRYLGRYYLVVKGLCPRGPEEAEIAGIQVDISGRKREEEDLRASLEAAETAS
                     RSKGDFLASMSHEIRTPLNAILGMTDLMLAMDVPQEHRDLLENVRISGQSLLEIVNDV
                     LDLSKIEAHRLVLDWDLFSPRQSLEVALRPMEPLARGKGLFLDLVVAPDVPAVVLGDE
                     IRFRQVVVNLVSNAIKYTDQGGVSLTVTARRPKGVSLWEEAKASRGDPGGSEPLSARK
                     SLELTVVVRDTGVGIPASRLPDLFQSFCRVEGAMARKRQGTGLGLFISKRLVEAMGGA
                     IGVESREGEGSTFSFTVRLGVAPEGSAISRSESRDREPVRGLPERLRVLLAEDNPMNS
                     KLARILLERKGWSVDGAENGHEALRRSRFRNYDLILMDVQMPEMDGLEATALIRQEEK
                     EGERVPIIAMTAYTMKGDRERCLEAGMDGYVSKPISPEELFRTIAEVLGQESREVPEP
                     LDLPRGGVDLSRLQRIVGGDRGILKDVIQEFLDRAPGMGQALGDALGRGALAEAQKAV
                     HKLRGSVSYFGVAALTALGERLEASCKGGDLEGAKALWPSLDRALGELMEEIKAIKSS
                     PAFSQESEGDSPGGLL"
     gene            complement(31671..32033)
                     /locus_tag="PL162_03500"
     CDS             complement(31671..32033)
                     /locus_tag="PL162_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQF91128.1"
                     /translation="MGKRILVVDDTAFMRFKLKAILEKGGHVVVGEATNGQEGVEKCA
                     LLRPDLVTLDVNMPDMDGLAALKLMKEQCPEVAVVMITAMGRQEIVMDAMMAGASDFI
                     VKPFEDEKVARAIAQLTS"
     gene            complement(32081..34225)
                     /locus_tag="PL162_03505"
     CDS             complement(32081..34225)
                     /locus_tag="PL162_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91129.1"
                     /translation="MLREFCDRMSFVLDGGVFSLLEERLGMELGRALVLQETGRSLAV
                     VTAVRFVPPVFSFSEGSQVLERAFSRENPLAPGGARVVSLSPEEADPFGEGWEEAARL
                     AGDQWCYYLLTSAPLSLELRNDLGAFARLVGLWHDHQKVVPLESRLSRLSYTLMAARN
                     AIASIFEPMPVEYFATFLGDVLREGVFSRRLAVLEDRGKEWRHLWGEPRYEPARQGIF
                     RETRILPSPLMVDEEHEGVLGDANLAALRALGIHVVLPLMGGPHRLFALMGWDRAPSE
                     EDMNFLALFVHVASKALEISALHEENRRQIAEVSTKAFALEGIHSSTLHLMRQQSRHD
                     LLDAASDILKEMSQTSRLELVVWKPQAGAYVLHGVPAGNGRPPEEVVHRGILEGCTLP
                     REGLLSGAEMNFFCETHMPGLKKICQRQGLDRILLMGDQDKFYGVVGLAPAVTGKDYG
                     DPMALKTLAGSVVVGLERCQLLERVTDQRDALDRRLLFMRLLRDIAEKVQEINDLDRL
                     CESVESSLRLALGAQPPYLILRGDSNSGRAELLAWASKMEVPRVVTLLSGRPMLLTPL
                     WSGRRCWGTIAVSSLDRVPLHSEEAEELFSLLAPLVAPRLASLMESRAWRRGPFLDVD
                     AMVRQELRLELERLEAQGVDGELIVRPGRGPLNPGQEEMVRMTVVGWEWTWCLIPFGW
                     DEEMREIFPPQEGWKRASGRAEDGGVGLPPSL"
     gene            complement(34225..35847)
                     /locus_tag="PL162_03510"
     CDS             complement(34225..35847)
                     /locus_tag="PL162_03510"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HQF91130.1"
                     /translation="MTTPEVDGEEKIPERGAWIHVGSGFALRRTAQEVWIEVRRLPLA
                     PMEVLEALRSVAPEVRIAREEVFRAASVLGSHRVGVEEEGGEGASPRVVVDEAGMTCR
                     LYLSPGFYPLQGGGGGLRELLDREGVVFGVDHEALDRAWARATAGEAVCAVVARGVPP
                     GPGEDGRVEFFFSPPTGRPVEDASGNVDHYHLDLIVPVQEGAVVAMRFPPTKGRAGRT
                     VRGEEVPPRKGRPAPLDFGEGLYLSENGELVAKYSGALRWKGSRILLERVLVISGDVG
                     SATGNVSYDGPVLVKGSVRGGFAVEAGGDVEVVGSVEDAAVTSRQGKVSVRYGIQGRG
                     RAVVRAGGDVMARFIQEAEVECETLMVNEYILRSTVNARKGVLVEGGKGVITASEIRA
                     RGHVHAKDVRSFREGDTRIDIPGVPRRDLFRDHQALEKEIEALKDHLSALAAEMRALG
                     PGTPGIRDLLRGYIEKADALADREERFAELGDFLRKMKGDATFGLRGSCSCGVNLRLR
                     GISSVTQNAMSRVTLFYDANEDRVVAVESGGA"
     gene            complement(35979..>36349)
                     /locus_tag="PL162_03515"
     CDS             complement(35979..>36349)
                     /locus_tag="PL162_03515"
                     /inference="COORDINATES: protein motif:HMM:NF012410.2"
                     /note="molecular chaperone; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HQF91131.1"
                     /translation="LDKDVKEVRVSERLTSSPACLVGEVHDMTPQLEKLLRHMGRDLP
                     PVKRVLEINPGHPVLRGLRDLHAADAADPRIEEFAQVLLGQARLAEGTELPDPATFSR
                     QVAKLLEVALAVRRRESPGA"
BASE COUNT         6499 a        13060 c        10698 g         6092 t
ORIGIN      
        1 gcacgaggac ggtgcgcctc tccgcagcgg cggtgccgca gacccccttg cccagaggaa
       61 tgcgggaaca ggcgggcttg ccccagaagg gccccagcac caactcccct tcccggaaga
      121 gatagaaccc cgcccagttg atgcgctcca tctggtgccc caacagagcc gccgcattgg
      181 ccaaattcgc ccccgcatcg ggctcccctt ccagaagaga ctccaggagg cgttccaacg
      241 tccgataaaa gagcttccga tccggcgtgt ctattttttt ggctcggtac accgcgtttt
      301 ccctccttcg agaccgtgcg tggcacacgt tttcccttgg ttgtgccccc gcttccccgt
      361 catggtacaa cactttcttc cgaagagcca cggggtgccg gtacgtggtg tcggcacaaa
      421 attccgccgt tcggccctcg cccccggagg ccccggggcc catactggaa ctccaggtgg
      481 gtctttcgag gagggttttg ctgtgggatt tcggatcatg gtggtgatgt ttcacacgtg
      541 ggggttcctc tacgtgttct ggcgcctggg ggccctcttc ccctccggaa gccggagcag
      601 gaaactcctc catgcggggg gtgtcctcct ctggagcgcc cttcctctgg ggctctccgg
      661 ggagagattt ttatctccgt cggtggccct cttcctgctg gatctggggt tctacgtcat
      721 gcccgtgctg ctccacgccg ccatggtggc gctcctcatc gacctgtacc acgtgctttc
      781 ctggatgttt cccctgccga agatggccct cctccaacgg cggcgcctct gggcctgggg
      841 ggggcttctg gtggcgctgg tggtcaccgg gggctatctc aacgcccgat ggctccggca
      901 acacgatatg accgtcaccc tcccctggtt tgccccctct cctccccccg cccctttgcg
      961 cctcgcggtg gtcaccgacc tccacgccgg cgccatggtg catggcggcc atctgcgccg
     1021 catcgtggac ctcatcgtgg ccacgagccc cgacgcggtg ctcttggtgg gcgacattct
     1081 ggacggccac atcggccagg cccgaaaagc ggagctggcg gaggagctcc ggcggatccc
     1141 tgcccccctg ggcgtttttg ccgtgctggg gaaccacgag ttctacgcgg gcatcgacaa
     1201 cgcccgggcg tatctccgcg agggtcatgt gcgggtgctg gaagacgagg cggtggcgct
     1261 ggggaaccgc ctgctcctgg tggggcgcat cgacctccag gggaggatct tcgggcatcg
     1321 gcgtcggccc ctggccaagc tcctcccctc ttctcgagac gagggaggct tgcccgcagt
     1381 ggtgttggac cacgcgcccc acttcgacga agccgccacc gccggggtgg cgctgcaatt
     1441 ttccggccac acccaccacg gccagctttt ccctttcaac ctcctggtcc gtcgcctctt
     1501 tggcttttcc tcggggtggc atcatcagag gaagaccctg ttttacgtct ctccgggatc
     1561 ggggacctgg gggcctccgg tgcgcaccac cgcccgtccc gaggtggtgc tttgcaccgt
     1621 gcgcttttct tccgccacag acgtggcggt tttagagccg gaagcgcgca ctggaagcgc
     1681 cgggagctcc ggagcgtggg gagctcctcc ggcggtttcc ccggccccta gcccggaatg
     1741 atccagaaac cgtcctgcag gggagagagg aattcgcacc cctgggcccc cacccgcacc
     1801 atctcctcct gccccacata tccccgggag gtgcgaacgc caaactccag cgtgtacacc
     1861 tgtccttcct ccacgggacg ggtcaccaga tcgccgtaac gttcccaccg gggccccaga
     1921 agagcccccc catcgtgggc ccgggtgccc acctggtgcc ccagggcgtg ctggaactcc
     1981 ggatatcccg cctcctccag cgcctgccgg gccacccggt ccacctccca ccccgccacg
     2041 ccgggacgga gaatcgccgc cgcgctccgg atgcctccct ggatcaccgc gagggcccgc
     2101 tccaactccg gcggaatccg gtcctcttcc ggtggtttga cataccacat gcgttgcaga
     2161 tccgagctgt acccctcccg gcgcacgcca aaatcgagat ggagggcgtg ccccggcaag
     2221 atgggaagct cgggaggggc gccgtgtccc gcctccacgt ggggccccga atccaccgcc
     2281 ggatctcccg ccgggctcca ggcgaacccc aggccccgcc gggccatctc ctcgtgaaga
     2341 aagtcggcca ccccccgcat ggtccacccc ggacgcaccg aacgggtgac gaggtcgaag
     2401 agctccagcg tctctccgat ggccccccgg atgcggcgca attcctccgg ggtctttctc
     2461 ccccggagcc tcgaaatgac cggttccgcc gagacgaagc gccccggata gggcgtgccc
     2521 tccagcagag cccccaagcg ctggtacatg cccagcgtca ggccgtcggc ggcggagctc
     2581 cacggagagt agttgagacc gatgctttgg ggatccatct cttccagcac cctgcgcagg
     2641 ggttccgcca acgaggtggt gtaggtttcc acccggtcgt agacccccag ggcctccacg
     2701 tcgaaacggt caaagctcgc caccacggcg accttccggc catccgcagc cagaagaaag
     2761 gccgcctgcc ccaccacatc gcaccccgcg atgagctcca gcacggggtc acattgttcg
     2821 gcggtttccc gaacaaacgt caaccaacag tcgattttcg tctcctgaag aatccccacc
     2881 gcctgggttg tcttttctct tacgagcacc gcgccccacc tccatggaat atggtaagat
     2941 ccatcctagc gaaggcccgg ccttccgtcc actgtccgga agatgcgttg agcgcctcga
     3001 aataggaaat ctccccgccg aaagaaccgc gccgagcccc tcgcccatca cgtcacacct
     3061 cagggaggat cgccatggaa ctcttcaccg tggacgcttt caccgacaaa ccctttacgg
     3121 gaaatcccgc aggggtttgt ttcctcgacg gcccccggga cacggcctgg atgggggcgg
     3181 tggcccggga gatgaactta tctgaaacgg cctttctcct ccgggagggc accaactggc
     3241 gcctgcgctg gttcaccccc cgggtggagg tggacctctg cggccacgcc accctggcca
     3301 ccgcccacgt cttgcgggaa aaaggcgttc tccgccccgg ggaaacggtg cggttccata
     3361 cgcgcagcgg cgaactctcc gcctccgccg aagaggggcg tatcgtgctg gacttccccg
     3421 ccaaggaaat tgaggcgtgt ccgccccccg agggccttct ggaagccctg gagatctccg
     3481 atcccctctt tgtcgggaaa aacatcttcg actatctggt ggaagtggga tccgacgccc
     3541 aggtccggag cctcgccccc gattttcccc gcctcgccca cctcgaagcc cggggcacca
     3601 tggtcaccgc ccggagctcg gagtccgggt tcgattgcgt ctcccggttc ttcgcccccc
     3661 gggtgggggt ccccgaggat ccggtgacgg ggtccgccca ctgcgccctg ggcccctatt
     3721 ggggagcgaa actcgggaag acccgcattt ccgcctttca ggcctcggaa cgaggcggcg
     3781 ccatggaggt ggttcttgtc cttccccgcg tgctgctcaa ggggcaggcg gtcaccgtcc
     3841 ttcgagggca cctggaggcc tagggctgga ggaccgccgc cgtttccgga gaaagaaccc
     3901 aacgcccccc gagaaccgcc catcgtccct cctcggcagg cggagtcttc ccggagaggg
     3961 gtgctttccg cattcatttt ccccgtcggg gcaggtagat ggggttctgc cccccgggga
     4021 gtttttggaa aatacgtggc gttttcttca cgcacatgct atcatgcgcc gcaaggtggc
     4081 ctttgccccc catcttcgac aaagaggtga ttttttggac acacgcggac gttttgttcg
     4141 gacgatcttc gacgcggtgc ttctggtttt tgccctgggg tggtccgccg gagcccaaac
     4201 ctcggaggac acaaccctct cccccgatgc gatccaggct gcggggaaag cgcttctggc
     4261 gggaacctcc ggggacgctc cggtggagcg ggtgctggtg tactacctcc acggcacagc
     4321 gcgttgcccc acctgtcaga acatcgaaaa gttcacctac gaggccgtca ccgcgaactt
     4381 cggggcggaa ctcgcctccg gagcgatgga gtggcacagc ctggacgtca tgaccgacga
     4441 acatcggcac ttcgtcgagg acttccagct cgtggcccag tccgtggtgc tggtgcggat
     4501 ggccggagac cggatggagg catggaaaaa cctggagcag gtctggcccc tggcccacca
     4561 gaaagaggcc tttctggcct acgtcaccga ggaactgcgg gcctttctgg aaggagacac
     4621 cccccggtaa cgttccttga gagcgccccc cgtcgcccct tcggcgtggc gccgccacgc
     4681 tccggaaaac ttccggagaa ctcctccccg gtcgaagccc cctaggcaaa caacgctccg
     4741 aagagcatcc ccgctccgga ggacaggagg atcaccaacc ccacatagac caccgttttc
     4801 ttcgttccca aaatgctccg gatcaccagc atgctgggca gggagagggc agggcccgcc
     4861 agcagcaggg ccagggcggg gcccttcccc atgccggcgc cgaggagccc ctgcaggatg
     4921 ggcacttcgg tcagcgtggc gaaatacatg aacgccgcca cgatggacga gaagagattg
     4981 gcgagaaggg agttcccccc caccaacgcc gccacccact ggctgggaat cagggcttcg
     5041 tgcccggggc gccccagaag aaatcccgcc gccagcacgc cgccgaagag caggggcacc
     5101 acctggaggg catattcccc cgtggagccg taccatgcgg cccgggcgtc cggggtaaac
     5161 cacagacgag ctgccagcac ggtggatatc agcgcggcgc ccgcaatcca ccatttgacc
     5221 tgatagacgg cggcccacac ccccagagga attttcggcg ccgcccagtt ggcaaagacc
     5281 agaaagacca ccatgcttgc catgaaaaag accgtctgcc acaggggccg gtccgcttcc
     5341 ggcacgggca tgtcggcaaa ggcctgttgg cgctcttgct cctcgtgacg gaagaggaag
     5401 gccatcagca cgccgatgac cacgctgaac cccacggccc ccacggcccg ggcgatgccc
     5461 atctccatgc cgagcacccg ggcggtcatg acgatggcga gcacgttgat ggccgggccg
     5521 gaatacagaa aggctgaggc ggggccgaga ccggcgccgc gcttgtagat ccccgcgaag
     5581 atcgggagca cggtgcagga gcacaccgcc aggaccgtgc ccgagatcgc cgccaccgtg
     5641 taggccaccc cgcgcgaggc cttcgccccc agatagcgca gcacggactg ctgggagaca
     5701 aagacgctga tggctccggc gatgaagaat gcgggcacca ggcagaacag cacgtgttcc
     5761 cgggcgtatt cgtggagcat ggccagcgct tcgatggccg ccccctgcac tctcgggctg
     5821 tccacgggca aaaagtagaa caccaggaac atggcggcga gagagaaaaa cttctttacc
     5881 tcgtcgttca tcttacaact cctttccatt tttgtgccgc gtacctcaca ttatgtgaga
     5941 tttttgccac ataaagaaga aaaacaaaag tggtcttctt ccgctccagg cctgaggtgc
     6001 catggagccg gaactttttg cgttcttttc gccccccttc cccccgaagg ctccggagaa
     6061 agcacgttct ccgccacgca ccgcaggagc ggaggcccgg cacccacgga attcccctca
     6121 cctttcgggg tccgcctccg gtcccgaggc atcgatcaca cgtggtcgtc ttcagcgccg
     6181 gagcacccgc acggagcccc gcacaccacc cgttccagac aggccagaaa ggggagcagg
     6241 caccgcatcc tcagccgata gaacacgaag agcccttctt ttcggtcctc aatgacgcca
     6301 aagcttttga gcaccgccag atgtttgctc accgtcgtcc ggtcacagga aaaggcgtcg
     6361 cagatcttac acgcgcaacg ctccccctcg ccgagcatct ccacgatgga aagccggatc
     6421 ggatgcgcca ccgccttgag aatgttcgat tttgcctgct gatactctcc ctggcggggg
     6481 gaagctccgc aggaaggctc gtaaaggttc tctgtcacct ccgtgtgcct ccttcgtaac
     6541 gcaatttttg aggacgtggg accaggagca gagcaacggc gatgttccgg gatccaagga
     6601 gatcccggaa gtaccctggg atcgaatgcc ggggcaaaaa atcttccggc gtgccggaac
     6661 aatggctttt tgggataacg tggcgatttt accacattgt tcgcaacacc gcacgccccc
     6721 ggacgcttcc ccatgcaagg ccctccaaat gctcttgcgg cggagcatca tggcttccga
     6781 agggaaggag gagccccggg agatcgcctg gagtcctttt ttccaaaaga gctgttccct
     6841 cttcgctcgg gcatccgaaa gatgcggtaa cgggtgtaga atgggacccg aggcatcatc
     6901 cgttcaaccc tcgaagatgg aaaaatcaga tggcgcagag cggtggggcc ttgctcggaa
     6961 aaaccttggg agctggagaa cgacgggagg ggagaaaacg ccatgcaggt gacgcgaaaa
     7021 gacctggacg acatggaatc ccagggcatt ctcacccccg gccaggggga gctcctgtgg
     7081 cgggcgctgc gggaaaaggg ggcagggagg ccccgctttg atctggccca cgtgctctac
     7141 tatctcggcg ccctggtggt catgggggcc atgacctatt tcatgaccct gggatgggct
     7201 tccttcggcg gcggaggact ggcgtccgtg gccgccgcct acatgctcct tttcgccatg
     7261 gcggggcgaa cctactttgc cgctccccaa acccgggtag cgggggggct cctttggacg
     7321 ctggccctcg ccatggtgcc cctcctggtc tacggtctcc aggagtccct ggggctttgg
     7381 ccccaggagt tccccggggc gtaccgggat ttctacgtct gggtccgagg agggtggttc
     7441 tggatggaag tcgccaccct gggggtgggc gccctgatcc tgcgtcgggt gtcctttcct
     7501 tttttgctca tgcccatctc tctggcgctc tactttcttt ccatggacct ggtgcccctg
     7561 atctacggcc aggagttcac ctgggaccaa cgcttgtggt tttccgtctg gttcggcctg
     7621 gccctttgcg gcggcgccta cgccgtggac ctgaaacgtc tccgccacgg cggcgaggac
     7681 ttcgccccat ggctctatct tttcggcgcc acggccctct ggggagggct ctcccttctg
     7741 gacagcggca gcgagctacg acgcctggga tacgccacga tcaacgtgct gttgatcttt
     7801 ttttcgctgc tggtagatcg gggagtcttt ctcgtgtacg gttctctggg gtttttcggc
     7861 tatctgtcct atctgagcta cacggttttt gccgactccc tgttctttcc cttcgtactc
     7921 tcggcgctgg gggtggtcct catcctagca ggagtccaat accgcaaata cgaagatcac
     7981 atcaaaaagg gcctccggcg ccttctgcct ccgggaatgg ccccctggat tcccgtggcc
     8041 taccgggagg acgagtgagg ccccatgctt cggcgcgcga aaggagcttg tcctcctctc
     8101 gcgcgccgga agggctccct tgtccgtcgg cgcgtgtcct tcggacgcca gggttcttga
     8161 cgacaagacc gcactttgcc ggaaacgaaa accggcccgt ggcgggagag aagtaacgag
     8221 ccgttctttt catcacaacg aaagggggag aatgccgccc ctcccccttt cgtttccctc
     8281 cgtcatcgca gcaggacgta gaccgccccc accgccccca tcaccgtgag tatcagattt
     8341 ccccccgcca tcgagagcgc caccgccacc cccagcccca ccgccgccac gccggggtgc
     8401 cccggcagcg cccccagggc atcgggaatg agaagcgctc ccagcgccgc agcggggatc
     8461 tgctccagca cgtggcgcac cccggggggg agagaccagc ggtggagcag cacgaagggc
     8521 gccagccggg gaagataggt caccagagcc atgcccagaa taagcgccag gagcccttcg
     8581 tccttcatgc cccttcctcc tttggcgttc cccaggatcc caccagcgcc cccaacacca
     8641 tgccccccac cagagccgtg ctggatgaaa cgccggggag gtggaccaac gccagatgaa
     8701 atcctcccgc cagcagggcc accgcccccc gtcggggaag gcgccgcacc gcaggcacca
     8761 aaagccccac aaacatggca taaatggcga tgcccagact tgcccgaagc gtctccccta
     8821 gaaactctcc aagcaaaaag cccagcatcg taccccccac ccaggagcca taggccgccg
     8881 cctccagccc cagcacttcg gcggaggaag ggggcgtttc ccgcagagac gccagagaaa
     8941 agacctcatc cgtcacgccg aaggccagca gagggcgcag ggccccccga agtccggggc
     9001 accgggccgc cagggcggca ctcatgagaa aatggcggaa gttcaccaga aacgtggcca
     9061 gaatgatttc ccccatcccc gcccctccgc cccacagggc cagggccatg aactggctgg
     9121 cccccgcgaa gaccacgccg gaaaagagcg ccgcccccca cagggggatc cccccggtct
     9181 tggccaacac cccaaaggcc agggcgatgg gcacatatcc cacgatgatg ggaatgccgc
     9241 accggaatcc ccgccaaaag tccttcactc cgtgcgccat ggcgcgtcct ccccgctctc
     9301 cccttttcgc cccgcctccc cctccttcgg cttttccggg agaaggatcc ggggcatacc
     9361 tccccatttc gtcttttcac aaaagggggc taaaatttct ccagaatctc gttgttgtta
     9421 acataacacc gcagggcgct cgccgccatg caggcgtgca ccggcagcac catgaccaga
     9481 tcgcccacat ggcatcggga aaggaagtcc cggggcgcca ccaccacccc gtgctcctgg
     9541 gaaaggctcc ggacgtaggc tccctccagc gcagggcccc aaccgtctcc ctcggggcgg
     9601 gcgatccggc cgtagaccct ccggccatct tcttcaagaa attccttgga gaggtgcaca
     9661 ccgccgccgt agatcaccag ttcgccccgg tcctcgtgga cggccaccac gggacacgcc
     9721 accgccatgg cgatacggtc caccggcaca gtgccgatct gcacctgcat gaggtcgtaa
     9781 aagacgaagt tccccgggcg gatctcatcg ataccctcga agttttccgc caagacgcag
     9841 ctgggggtat ctcccagaga cgcgatgatg tccgaatgga tggggcggta cgcctctttt
     9901 agctccccca gggcccttcg ggcctcttcg tggacggcca ccacttctcg ggccccccgg
     9961 caggcatagg catgccccgc atgggacaga aagcccctaa aggtcatggc ctcccccgag
    10021 gccagggcct ccatgaggtg ctccatgcct tcccgatctc ccggagtcag tcccgtgcga
    10081 ccgtacccca cgtcgatctt gataaagacc cccaccggat gtcttaggtc ttttttaagg
    10141 tgatcgacgg tctccaggga ctccaccaag aggtttagac gaattctttt cgccagggcg
    10201 ttgataaggt ctctttcgag gacgttgacg ggaaaggcca cagtgatgtc ggaccactcc
    10261 tcggaaaagt actgggccat ttccaaagat gacacggcga ttttatctac gccgacgtgc
    10321 ttgaaccacc ttcccaccgc cagggattgg tgcgtcttga agtgagggcg aaagtccacc
    10381 ccccgatccc gggctttctg ggccatgaaa gcgatgtttt ccctgcaccg ggcctcatcg
    10441 atgaccaggg ttggcttcgt aatgggaatc atccttcgct cctttcccgg ctcctccggg
    10501 ggccaatggc ggccctttcc gcgccgtcca gcagaaagtc cacgagcgcg gtccatgctc
    10561 ccccgcgctc gcgacctgga agttcgctag atttccctcc atcgcccctc gccggggccc
    10621 ccgtcagggt cggatgagct ggggatcgtc caccacgccc atgtaggtga acttgccgtc
    10681 cttcaccact tggacctgca ccggcttcac cacttctccc gcttcggtga acccctgaat
    10741 gatccccgtg agaccatcgt agtccttcac tgcggcgatg gcgtcccgga cggccttgcg
    10801 gtcggtgccg ccggatcggg tgatggcgtc cacgatgatc atgagggcgt cgtaggccga
    10861 ggcccccacc atgtcgggct ccatggcgta cttctcccgg aagctcttga ggaactcctg
    10921 caccacgggg cgcgggtcgt cccggttcag gttggtgacg aggacaaagc cctcggcggc
    10981 gggaccggcg atctcgaaga gcttgggaga atcggcgcct tcctcgccga ggatggcggc
    11041 ggtgatgccc atctcccggg cctgcttcac cacggggccg gtctggaaat aatatccgaa
    11101 ggccacgagc acatccggag cgccttcctt gatcttggag agataggggc tgaaatcttt
    11161 ttctttggcg ggataggcct gctcgaagat cacctgggga gtgcccttcg ccgccacata
    11221 gtccttgaag ccctgggcga gggtcttgcc gaagtcgtta tcggcataga gcagggccac
    11281 ttttttgccc ttcagcatgg tgtccaccac gtaggctgcg gcacccccct ccactttccc
    11341 cagaaaaccg ttgcggaagg tgaattctcc ctcggtgaca tcggggtgga tggcgtaggc
    11401 cgccaccatg ggcacctctt cttcgttgaa aatcggcgtc gtcacccggg tgggcatgct
    11461 gtaggaccct cccaccaccg ccaccacctg atcctgctcg atgagcttct gggccagcgt
    11521 cacggattcc ttgggatccg ccgcgtcgtc gtagatcacc agctccagct tctttcccag
    11581 cacgccgccc ttggcgttca cctgctccgt cgccagctcc agggcgttct tcaccgaaag
    11641 gccgtccgcc gccgcaaacc ccgtgagggg cgccagcacg ccgatcttca gcgtttcctc
    11701 ggctcccgcc acgcccgctc ccagcaacac cgctcccgcc aaaaccaaag cacaacagcg
    11761 ccacaacttc atcgtatccc acctcccatc aaaatacggg acaccgcttc gtgtcccggg
    11821 aactctccca ttataagcca gacctagagg ccaaggtagg cctctcgcac ccgggcatcg
    11881 tgctccaact cctccgccgt gccggagagc accaggtttc ccgtctccag cacgtaggcc
    11941 cgatccgaga tggccaacga cgcccgggcg ttctgttcca ccagaagtac gggaagtccc
    12001 ttctccctgt tgaggcggcg cagcacggcg aagacctcgt ccaccagctt gggcatgagt
    12061 cccatggaga cctcgtccac cagaagcagg cgaggttccg ccaccagcgc ccgggcgatg
    12121 gcgagctgct gctgttcgcc tccggaaagg gtggaagcaa gttgccctcc ccgctcttgc
    12181 aagatgggaa acagggcata gagctcatcc agacggcgtc ggaagctctc ctcgccgatc
    12241 ttcagtccgt agaccccggc ccggaggttc tcccgcaccg agagctgggg aaagatccgg
    12301 gcccgttcgg ggacgatgcg aatgccccgc cgggcacggt ggtgcggtgc cagggaggag
    12361 atctcctccc cgtcgaacac gatactcccc gaagcgggga cccaccccat gagggccccc
    12421 agaagcgagg tttttcccgc tccgttggcg ccgatgacgg agacgagctc tccggggccc
    12481 acctccaagg agacgccgtg caccgcctcg atgtccccat aggacacccg cagattctcc
    12541 accttaagca tggcccgagg ccccctttcc cagataggcc tccaccaccc gggggtcggc
    12601 ggccacctcc gctccggtcc cttcggcgag cttgcgcccg tggtccagca ccacgatgcg
    12661 ctccgaaagg gccatggcca ccttcatgtt gtgttcgatg agcagcaccc ccacgccggc
    12721 gtcccgcacc ccccggacgt ggagctccat ctgccggatt tcttccgccc gaaggcccga
    12781 aaaagtctcg tcgagaagaa gcagtttggg gcggagagcc aacgcccggg ccagttcgag
    12841 gcggcgcaga ttgcccagcg gcaggagccc cgcccggcgg gaggcgaggt ccgcgaggcc
    12901 cacggaggcc aaaatctccc gggcttcttc ccggacgctc cttgtgcccc atcgccccag
    12961 aaggcacccc agggagccgt accgacgcag acccagggag acggagacgt tctccaacac
    13021 cgaaagggaa ggaaagggcc ggacgatctg aaaggtccgc cccacccccc gccgggccat
    13081 ttccgccgga gaaaccccgg tggtgtcggt gccatcgaag aatatccgtc ccgccgtggg
    13141 ggagaacatg ccggaaatca tgttgaagca ggtggttttt cccgccccgt tgggccccag
    13201 aagccccagg atttcccccg gatgcaggtc aaaggagaca tcttccaggg cctggagccc
    13261 gccgaagcgc ttggagagtc cccgcaccga aagcaggggc tcccgggaag aggcgtggtc
    13321 cttccgctcc acgtccgtca tgacacctca cccccttttc ggcgaaaaga aaagagcttg
    13381 cgcgccaggc tccccggccc caggagtccg ccaggctgaa accggatcat caggagcagc
    13441 agcgtggcgt agagcagcag ccggtactcc gtgagggggc ggaaaatttc ggggagcacc
    13501 cccaggatca gggcgccgag taccggtccc cacaagcttc ccatgccccc caacaccacc
    13561 atggaaagga gcatgacgga aacgggaaaa ccgaagtccg aggcgctcac gaaacgcatg
    13621 tggtgggcgt agagagcccc tccgagcccc gcaaaggtgg tgcccagcac gaaggccagg
    13681 agcttgaagc gcacggggga aacccccatg ctggcggcag cgccctcgtc gtcccgcagg
    13741 gcgaagcagg cgagccccgc ccaactccgg gtgaaacccc aggaaatcag gagggccagc
    13801 gccaggaaaa agaggcacag ggcgagaaac gtggggccct tgaggcgttc gccgaagagc
    13861 acgatgcggg gaatgttgcc gatccccagg gcgccgccga agaaggacac gtagagaaaa
    13921 agggactcca cgatgaaatt gatgccgatg gtggtgatgg cgagaaagtc ctcccgcacc
    13981 cggaggctcg gaagacccag taggagcccc gccgtggcgg tgatgaggag caccaggggc
    14041 agggcggccc agaaggagag atcgccccgg gtggcgagca gcgcggcggc ataggccccg
    14101 atgccgaaga acgccgcatg ccccagggag atctgccccg cgtagcccac gatgacgttg
    14161 agaccgcagg ccacgatggc gtggatggaa atgaagatgc cgagggtcac aaggtagctc
    14221 atggccatcc tcctccgtca ccgatgcccg aagagcccct gggggcgcca catgagcacc
    14281 gccaccatgg cgatgaaggc cagggcgtcc cggggcaggg gaatatccgc gtagccgatg
    14341 agcagggttt ccgccacgcc cagaaggagg gaggccccca ccgctccggg caccgacccc
    14401 aatcccccca ccacgatgag agccagggac ttatacgccg gcaccgcccc catggtggga
    14461 tagacctgat tgtagtagat ccccaccagc attcccgcca ccgccgccac ggcggagccc
    14521 agggcaaagg tcaccgccac cacgcgatgc acgggaatgc cacaggccgc cgccatgttc
    14581 cggtcctggg ccgtggcccg catggcgagc cccagctccg tccgggtggt gaggtaccac
    14641 aatcctccca acaccagcgc cgtgacgccg taaaccgaca ggagggggcc cgagaagcgc
    14701 acgcctccca gggtgatgtc cggaacggga agttccacag caaagggcag gatgtagggc
    14761 cccgccacca ggcggcagat ctcctccatg cccagcagca tggcgatgct gctgattagg
    14821 ggtacgaagg ggggaaagga gagcaacggc gtatagatcc accgctccat ggccaccccc
    14881 aggagggcgc acccccccat ggcccccaac gccgccagca cgaggctgcc ggaaagggaa
    14941 aaaatcccca aggccagata ggcccccacc gcgtagatgg cggcatgggc cacgtgcaaa
    15001 atgcgcaaga tgccgtacac caacgccagc cccaacgtga tgagggcata gatagacccc
    15061 atggccacgc cgctggcgca ctgttccaag aaaagatgca ccctctcacc cccgacccgt
    15121 cgtaccttct ttgtcaaaaa atttgattca cgttaaaaac attcccctcc gtcgggggcc
    15181 cgccccctcc ggctcatctc gggtttcggt gatagagaca aacatatttt aatcagactt
    15241 cgcggtgaaa ggattccccc ccgggccaaa accctcctcc cctccccccc ggagggaccc
    15301 caccagcttt cccagcaggc caaccaacgc ctcggggtcg atgggctttt ccacccccgc
    15361 gtccacttcc ccctccggtt cttgccccag gaccagaaag ggcctgcgaa agcccccctc
    15421 ttcgatctct cgaaggcccc gggccacctc cgcccccgat ccgtcggaga ggtgtccttc
    15481 taggatcacc gcgtcgtagg attcctctcg gaaacacctt ttcgcgtcct cccccgtggc
    15541 caccacgtcc gccttccagc cgccacgctc caggaggcgc cggaggatca gggcctgcat
    15601 ggggtcgtct tccaccacga ggatgcgccg cgacggcgcg aggggaaggg aaggggcaac
    15661 gacctgggga ggcgtcacca ggggaaagac cacggaaaaa aagaacgtgc tcccatgccc
    15721 ctcggagctc tcgaaggcca gggtcccccc catggcatcc acgatctggt gcgatatcgc
    15781 cagccccagc cccgtaccct gaaaacgctt ccgagacgac aggtcggcct ggaagaacgg
    15841 gcgaaagatg aggggcttct tttcctccga gatgccgacc cccgtatctc gcacctcgaa
    15901 aaagatctcc gccttacccc cctcccgctc ccgcccccgc acagtgaagg tgatgcgccc
    15961 tcggtcggtg aacttcaagg cgttgccgat gaggttcacc aggacttgac gcagcctcaa
    16021 gggatccccc cgaactcgat ccggcaccga gccctcttcg cgatgttcca ggataaggcc
    16081 cttgcgcaac gccgccacgt ccaaggtcca gaccacctgg acgcagaggt ctcgcacgga
    16141 aaaaagttcc tgggaaagtt cgaatcgccc cgcttctatg cgggaaaaat cgagcagatc
    16201 gctgatgatc tccatcaatg aacccgacga agaccgcacc gaggaaagga gatctcgctg
    16261 ctcccgggtg aggggcgtct ccagcagcag atccgtcatg ccgatgatgg cattcatggg
    16321 ggtccgaagc tcgtggctca tgttggccaa gaaggccccc ttggcccgac tggcctcttc
    16381 cgcccggtcc cgggcctccc ggagcccccg ttccagctcc gattgttcgg tgatgtcgcg
    16441 ccccaccgac acgagttccg tcacctctcc ccggaggtct ttcatgggga tacccgacac
    16501 ctcccagtac ccctctccca tccctcctac ggcgtcgtga aagacccgcc gctccaattt
    16561 accgctccgg agggcctcca cacaggggca cccctcgcag ggcttcgggg agctaaagag
    16621 ggcctcgtga caccgctcgc ccacacatcc cggaaagggg cgccgcaccg cctcgttggc
    16681 ccagaggatg cgaaggtggc catccaagtg aaaaatccga tcggtgaagc cgtccaggat
    16741 ggcccgaaag ctccgcatgc tctcttccag ggccccctcc gcccgcttcc gacgggacac
    16801 gtcgacgacg ctacctccca ccctcagggg gcgtcccttg gagtcccgct ccgtcacccg
    16861 cccccgtccc agcacccaca cggggcttcc ccggctatgg cgcagccgaa gctccaggtc
    16921 gaagcggtcg ctccgccccc cgaggatccg ccgcaacctc cgcgtcagcc ccggaaggtc
    16981 ctcgtcccag acgagggcct tggccgccgc caagtcggag agtagggctc cggggaggag
    17041 gtccagcatg tggacgaact cttcgtcgaa gacgacgtgg ccgcccggca agtgccactc
    17101 ccaggtgccc acgctcaccc cttccagcgc cctccgccga cgatcttccc gccccccatc
    17161 ccctcgctcg gagggtaggc gtcccgagag gtcggtgatt aggacccacc tccacccctt
    17221 ccggggaaag ggctctccgg acagggcccg atattccacc cgcacgggcc ggggcacccc
    17281 ccctcccaga gggagcacgg catcgaagga tcccccggcg gagagcgtca aggccactgc
    17341 cacagcgctc ttccaggatc ccgcatgctc cggggacacg aagtgctcca ccgtatgccc
    17401 cgtcaccgct tcccttcgaa ggcccgtgat gcgacagaag gcctcgttca cctcgacaaa
    17461 tcttcccgcc gggtccagga ggacataacc ttgatcgatc ccatccacca cggcgcgcag
    17521 gtgcccccag ggagtgcccg ggcgcgccga tcccttccat attttttttc gattccccgg
    17581 ggatcccatc ttcaggaact ttctcgggcc tggtcgacga ggaccccggg gtcgaggata
    17641 agggccaagc tcccgtcccc caggatggtg gcccccgtgg cgcccccgaa gcggaaggac
    17701 ccggggaggg gcttgaccaa aaactcctct tcttccaaca ggtcgtccac caccaaagcc
    17761 acctgatttc tgcccgaccc cacgatgacc agggggatca gatcgaactc gaagctcttt
    17821 tgcgccaggt ccaccgcgtg cccaaagagc cccgcttcgg cgggaaggag gggcagggcc
    17881 gccccccgta cccgcacgag gacgaagtcc gcataggcct gtagggccga tcggggcacc
    17941 ttcagcgtct cccgcaccga ttccagaggc accacgtagg tcccctcccc tatgcgcacc
    18001 cgaaggccgt ggatggacga cagggagagg gggatacgca aagtaaagca cgatccccgg
    18061 cccacctcgg aggctacagt gatccgcacc cccacgttct ccagggaaga gaggaccgca
    18121 tccatgccca ccccacgccc cgagagattc gtgacttttt ccgccgtgct gaatcccggc
    18181 aggaatatta cttcgaggat gtcatgatcg ctgcgccgcg ccgcctcttc cggggaaaag
    18241 atgccccgct ccacggcctt gacccggagt ctggcggggt cgatgccccg gccgtcgtct
    18301 cgcacatcga tgaaggcata tcccccctgg gtgtaagccc gcaacgtcac cgtcccccgg
    18361 ggagtcttac ctccggcgac ccgctcttgg gagggttcca agccgtgatc catgctgtta
    18421 cggaccatgt gggtcagagg gtccgtcaat ttgtccagga cggcccggtc aagctccgtc
    18481 tcgcccccct ccaccgcaag gtcgacttcc ttccccacgt cccgggccag gtctcgcacc
    18541 acccggcgaa agcggttgaa gagggtctcc acgggggcca tgcgcatggc catgacggcg
    18601 gcctgaagct ccgccgtgag acgctccaga ttcacccccc gggccttgag ctcgttggcg
    18661 aagtgggggg aaacctcccc caaccgccgg gccacgtgga gaaaggcgtt cttcaggctc
    18721 accagctccc ccgccagatt caggagcttg tccaccttgc ctcggtccac ccggatgctg
    18781 gccgccgcca gggcatcccc ccgatcggtg gcgtggtcgg cctcccgaga ggccccccct
    18841 ccttctctgg gggcctcgcg cgccaccccc tggggaggaa cgggggcgtg catcccctcc
    18901 agaagagccg ccagggccgc cacgcccttt tccgtggcgg ggtcctgggc cgaggtcgca
    18961 tgacgacata gggcctccag ggggtccagg gaacggcgca gggccgccat ctcctccggt
    19021 tccagcggaa tccccctttc caggcgatcc ctcaccaggc gctcccccgc agaggccaaa
    19081 atcctgatct ggtcctgccc cgccaagcgc gagaagcccc tcagcgaacg atacccccgg
    19141 aagagagcct ccagggcgcc cccgtccccc ccttccatcc gggagaagcg ctcccgcaac
    19201 aggagcacgt agcccaacat ctcttccaga gcgttttcca gaaaggcccc gtccaggacc
    19261 accgcgccct tactccccgg ggaggctcct tccctctcca agcgcaccgg gggggaggcc
    19321 gccgcctttt cccggagatc ttccagcaac acgtccttca cgtccaccac gtcgcgcacc
    19381 cgggcctcca ggagctcccg gggcttagaa gacagcaaaa agaccaggat acggaaggac
    19441 cccgatcccg ccgccgactc cacggcatcg ggatctttct gaaggtcctc catgcggggg
    19501 acggagcgga agacctctcc caaagatccc aggaggcgca acaccatgaa ggcccggacc
    19561 acgcgcaggg ggcattcatc caccagggcg acctccaggc ggaagggttg gaggcctcgc
    19621 ttcgccgcct cttccagggt gtctttttcg ttcgggggca aggatacggc cttggggccc
    19681 cctcggcggc gggcctggcc tccgtggtgg gagtgaatca gggcgtgaag gcgctctaac
    19741 acctccccca ccgccactcc ggtgtcgtct ccggcgcggg ccacttcgtc cagagcgttg
    19801 cgaagcaagt cgtggctcac gaagagcata cccaggagct gctcgccgat ctccaccttg
    19861 ccatcccgga tgtcttgcag gagatcctcc gccgcgtggg tcaggcgctg catgttggaa
    19921 aaccccatgg cgccggacat gcccttgatg gtgtgcgccg cccggaaggc ttcccccacc
    19981 gccataaggt cccccggcgc ggcgtccaac ttcaggaggg cgccgtggaa ggactccagg
    20041 acctccaggg cctcgtccag gaaaagcttg aggtactgct ccctttcggt catcacggga
    20101 cgagccccac ttctcgcagt tgggtctcga aggaatcccg atccaccggc tttaccaagt
    20161 agccgtcgca ctcgttggcg aaggcccgcc ggacgttttc gatgtcccct tgggaggtca
    20221 ccataaacac ccgagccgcc tcgtgaggat ggatgccccg ggattcctcc gcctcccgga
    20281 tttctcgcag caactgctgg ccgtccgcat cgggaaggcc aatgtcaaga cagatcaggt
    20341 cgtagggctc tccctcttcc agggcgatcc ggagggcttc ccgggcttcg gcaccgctca
    20401 cggccacgtc caccgggccg taggggtcca agagaaggga caggaccttg cggttcaaaa
    20461 actcgtcctc caccaccaaa gatttcatag ttttttcacc acctcgacga ttttggccgc
    20521 gatgcgatag gacggcaggg ccacatcggc tccccctcgc ctcagggctt cggcgggcat
    20581 gccgaagacc accgccgtct cctccgactc cacgatgccg tatccccccg agtgtcggat
    20641 ggctcccatg ccctccgctc catcgtcccc catgcccgtc atgaggaccc ccacggtgcg
    20701 agggccgaag gcttcccgca cggagtgcat catgacgttc acggagggga tgaaggtgga
    20761 tcggggctgg gaggggaggg aaagtttgat ctcgtcgccc cgtcgatcca cgcaaagctg
    20821 gtaccccccc cgccccacgt acaccacccc gggagtcacc gccatgcccc gatcggcttc
    20881 caccacccgc agggcgcagg aggcgtccag cctctcggcg tacattcccg tgaagtgggg
    20941 aggcatgtgt tgcaccacga agaccgcagc gttcagatcc cccggaagct tgggcaagac
    21001 gtcgtagatg gtacggggcc ccccggtgga gatgcccaag gccacagcgg aaaagggcgc
    21061 gccgcctttg ggagcgtccc ttttaggggc agggctagac cggggcgtcc ggggccctac
    21121 ccttcgaggc cgagaggccg ccgccgcccg aatcttcgac accagctcgt cccgcaccgt
    21181 ctccagggag cgcgagatgg tccccccagg cttttccacg caatcgaagg ccccgagctc
    21241 cagggcctcg aaggtcacca aggccccctc tcgggagagg gaagatacca ccaggacctg
    21301 ggccaactcc tcttccaaga ccagctgaag ggccgaaagg ccgtccatgc ggggcatgtt
    21361 gagatccatg gtcaccacgt cggggcgcag gtcccgggcc ttctccaccg cttccaggcc
    21421 gtcccgggcg atgcccacca cttcaaggcc cggctcttct tccagcaggc gacagaaggt
    21481 ccgccgcatg agggccgaat cgtccgccac caacacccgg acggagtggc ggctctccaa
    21541 ggggatcacc gggccttccg caggcgccgc ccgggcttga gaggctccgg agccccttcg
    21601 gtggagggcg caggggcctc gttcacccct tcttcggcgg aggctcgatc cgccagggcc
    21661 cccacccggg aactctcccg gggagagagc accgtcccga gatcaagcac caggaccacg
    21721 ctcccgtccc cccgacgggc cacccggtgc accgcctccc gagatccctt ctcgcctccc
    21781 cggggtggaa gcaggaggtt ttctcggtca aagcgctcca ctcccaccac cgtatccacc
    21841 acaaagccca cggtgactcc cgcgttttcg aacacaagga cgcgagggtc gccgtcaccc
    21901 tcgccgccgg agtccgcgat ctccagcttc cgccccagat gcaccacggg aaggacttcg
    21961 ccccggaggt tcatcacacc cttcacgaag tgaggggatc gggggatggg ggtcacggag
    22021 ggcatgcgac agatctcccg aatttgttcg atgggcacgc cgtagggctg ccctcccacc
    22081 gtaaagagca ccagggggac atgggtccct tgatcgatgc ccccggggag ggcgttctcc
    22141 gggctctcct ttccgccggt gggctctccc cggccctcca gaagggcctc ggggttcaag
    22201 agcaccgtga tccggtcctt ttcttcgatg atgcctccca cggtggcgtt ggcccgggcc
    22261 accagcgggg gaggcggggt catggcgccc tccgtcactc gaaggacctc gtggatccga
    22321 tccaccacga agcccgtgct cgccgatccc gaccccagca ccaccacctt ggtgaactcg
    22381 tccaccgccg tctcggccat gcccaagcgc atcctcaggt ccaccacagg aagggtgtgc
    22441 ccccgaaggt tcatcacccc tttgacccag ggcggggtct cagggaaagg cgtggggggg
    22501 gtgtagcgca cgatctcccg cacctcgacg atgggaaagg cgtactcctg ccctcccact
    22561 tcgaaggtca ccacctggac ccggggttct cgcgggccct cctccgccgc cagggatccc
    22621 gcctcggatc cctggcggcg gtcccggcgc tcccacgagg tcacccccgt aaggagttct
    22681 tggggattga tgaccatgaa gacttcttcc cgaaagcgaa tcacccccgc aaggagctcg
    22741 gaggaccctt ctcccccggg gctgcgttcc acctggcctt cttccacccg gagaacctgg
    22801 gaggtgcggt ccaccgccac tcccaccccc aggccgtcaa aatcgaaaac caccaccttg
    22861 cgcccctcct cggaggatcg aggaaggccc aggcgggtcc tcagatccag cacgggaagc
    22921 acctctcccc ggaggttcgc cagcccttcc acgcactcgg gagaccgggg caaccgggtg
    22981 acggcgggaa gccgcacgat ctcccgcacc gccgccacgg gcaccgcgta caccacttcc
    23041 cccagggaaa aggtcacgta ctgcccggca ccagccacga gctacacccc cgcttcgctc
    23101 tggagttcgt cggcgatgat ggcgatctcc tccacggccc gggatagctc ttccatgccc
    23161 cgggcctgct ccgccgcagc cttggcggcc tcttccacgg cggcggctcc ctgctgggag
    23221 gcggcgctga tgttttccac ggaagcgtgg gcctggcggg tgcactcctc tatggaatta
    23281 accacgtcgg cgatgcgctc gtagagcttg gaggtctcct gaaaaagccg gacgaggtct
    23341 tcgatgaggc gcaggctgat ggctcccttg gcattctccg caccggattg ccgggacgcg
    23401 tcggagacat cgctgagcac cgccccgatc tggcgctgca tggcaaagac caggtccttg
    23461 atgcgatccg cgttctctcc cgactccccc gccaggttgc ggatgtcgcc tgccaccacg
    23521 gaaaatcccc ggccaaaatc tcccgcccgg gccgcctcga tgaacccatt caccgccaac
    23581 atgttggtct ggatggtgac cttgtcgatg gtggccacgg tcttgtcgat acgccgcccc
    23641 atgttctcca gatccgacag gttcaccgcc gtgccctgga gcatgcgaaa gaccttctga
    23701 actcctttga tgatatcatc gatcttgccc cggagctcct gggccctgtc gtggcacgtg
    23761 tggatcaacg cgatacgcct cgccaccgtg ccctgcatgg tggccgtggt ggtggcgatg
    23821 tccaccgaga gccgcgtggc ggattccgcc gcctgagcca ccgccgtggc gctggtggcg
    23881 aggcgatcca tgctcccggc cacctgccgg gcgctggcgg aggattcctg gaggttcgag
    23941 gagagttgct ccgccgccgc cgccacccgc tccgaggatt tatcggtctg cgtggagttt
    24001 ttcagctcgt cggccagatc caccaattcc tgggtggcgc tggcgatctc ctggagcgcc
    24061 cgggattgct cccgtaccgc cttggcgcac tcccccgcca gggtggtgga aatctgggag
    24121 ctctcggtca ccaccctcgc gtcttccagg aaagcccggg ccttttccgc gatatccgcg
    24181 atgatcgtca gagaggcttc cacccccttg agggccccca gggcttcgcc gtcgaggaag
    24241 gccaacgtct cccccgcctc gtctccccgg gccacctggg cgtccgaggc ccccacgacc
    24301 ccctcgatgt cctccaccac ggtggacacc aggacgttga tgccccccac cacgtcccgg
    24361 atctcccggg ccgacttctc ggagacctcc gccaggtttc gcacctcgtc cgccaccacg
    24421 gcaaagccgc taccgtgctc ccccgcccgg gccgcctcta tggcggcgtt gagggccagc
    24481 aagttcgtct gatcggctat catcaccacg gtcttgacga tgtcctccac ctggcgagat
    24541 tggtcgtgga gacgccgcac cgtctccgcc gaggccttgt tcctcgcggt ggcctcttga
    24601 atggcggcgt tggcctcgcc gatgtttttc gtggcatcgc cggtgatccc gcgaatggcc
    24661 gtcattttat gatgggcctc gtggccgaga tccacaaagg ccccggcgct cttttccacc
    24721 atgaccgtgc gcccgtgggc ctcggcggcg gcgccctccg cctggtgggc cccggcggcg
    24781 atctgctgca tggcccggtc cagctgctcg gcggaactcg acgcctcctc cactccggca
    24841 aggagctctt ccgctgcggc ggaaatgcgc tccgccatgg cttgacgctt ggcgaaggtc
    24901 cgggccctca cccgttcctg ggcccgccgg cgggccaggt cacgcttggc cgccaggtct
    24961 ccgtcgctcc cctcttcccg gggcaccaac cccgtgctcc ggcggtcctt cgctccctcc
    25021 cggttttcct ccaggtgctt caaggattcg ggcacttcca gtcgtttcgc catcctcgtc
    25081 ccactcctct cccaccgatc acagcaccgt cacgtccccg aggaaaagct ctccccgatc
    25141 cccttcttcc cactcttccc agggggcccc gggaaggacc cccgacggga tggtcatctc
    25201 ccccgtcttc cccgtggcct cgtcctcggc ggccaccgcc aggactccgc tggcatccac
    25261 ggcaaaggtc accgccagct ccgcttcgcc ctgggcccaa gccgtggggg cgcaaaactc
    25321 caagaccccc agaggcacca ggcgatcatc ccgacgctgg agcacctcca ccgtcacctc
    25381 tttctgtccc tcccgcgcgt tggtaaagag cctcaccgcc gtcacgggat agaccgtgcc
    25441 ccggtccacc agggggacga actcccctcg gtcgtcccgg actcccaagg cgtggggcag
    25501 tacgtcccgg agctccacgt tttccaagcg accctccatg atccccgcgc acaccgccgc
    25561 tcccagggcc accacttcgt cggggttgag ctctccccgc accgaggccc ggggaaaaag
    25621 ttcttccatc tctcgccgaa accggggcat acgagaggat cccccggcgt agatcacccc
    25681 gtcgatgccc tccggggcga tatgggattc ttccagcacg ctttgcacca gccctcggat
    25741 cttggcgaaa agcccctcgg ccaaccgttc gaaggtcttc cgggaaatct ccgtctggac
    25801 gtggatcgga ccctcgggcc ccgaggcgat gtagggcaat aggagcatac tctcctccac
    25861 ggaggaaagg tcgatcttca cccgctccac cccccgcatg acggaggccc gggccacggg
    25921 gtccgccccc agatccaccc cccggcctcg ccgcacttcc tccaagaccc actcccccag
    25981 gaggtcgtca aaatccgccc ccccgagctc cgtgctccct ccggtggccc gcacttgatg
    26041 gagtcctttc gaggaatgga gcacggtgat gtcaaaggtt ccaccgccga ggtccaagac
    26101 gatgaaagtg ccctcttcga cctgggcggg gcgataggcc aaggccgccg ccgtgggttc
    26161 gttcagcagg cgcaccacgt gaatcccccc ccgagccgcc gcctccaaca catcgtggcg
    26221 ctgggcgtcg tcgaagtagg cggggaccgt caccacggcc tgggtcaccg tctccttgag
    26281 gtaatcctcg gcgtagcgtc ggagcttggc aaacacgagt ccccccaccc ctgccgggga
    26341 aagctctcgg ccaaagaccc ggtagggggc ggggtttccc atgcggcgct tcactctggc
    26401 gatagtctga tccgggcgca acaaccctcc cgatcgggcc agctccccca cgagggcctc
    26461 gtggggggag cggaagcaga ccacggaggg ggtcatccgc tctccccgct cgttcacgac
    26521 gagctcgggg ccccgaggag tcaggtaggc cacggaggag ttggtggtgc cgaagtcgat
    26581 gccgacgatc acgacggtcc atctcccaga caggccaagt tctcccgggc ctccgcgcac
    26641 tgcccatcct gccgcaggga aaggcgtagc atctcccggg cgtcctcttc gaagccgtgg
    26701 tccgacagga tcaccgccgc attgttcagc gcccgggccg ccagaggagg gggggaggcc
    26761 tccgccaccc gcaggaacag gtgaaaggcc tggaggaact tgccctgttc ttccaaccgg
    26821 cccgcctctc gatagaccga ttccatcgaa tcacccagaa gggattccag ggaagatgcg
    26881 agggtcatgg gcgaatcctc cgccagagga tccgacagag gtcatccacg tcctgggccc
    26941 cccggctacg aggggcgaag gccatgatcg gccgtcccgc cgccgctgcc tgggaaaact
    27001 tcacatccag gcgcagggga gggagcatca tctcctcgcc gaagtttctc ctgagctctt
    27061 ccaccgcctc ccgggccccc cgggtacgca gatccgccat ggtgggggcc acccccccga
    27121 ggcgcaacac gggattcgcc gtggcggaaa ctttgtaaaa cgacgccatc atccgggcca
    27181 cccccgccag ggaaagaaaa tcggtgcgca cgggcaccac cagcacatcc gccgccacaa
    27241 gggccatccg ccccaacctc cccgccgccg ggggagagtc caccacgatg aaggcgtagc
    27301 ccctcagaca catggacagg taatcccgca ggagggcatc ccctccggcc tcttccctct
    27361 ccaggccatc cagctcttcg tccgatgcca acaggtccaa atctcccagg ctccggagcc
    27421 gaggatcgcg ccccccttcc agaaagcgac gcagttcctt cgccgtgccc ccaggctccc
    27481 cccgggctcc ctccccccgg acgcccaaac acagccccgc atgtccctgg gggtccaggt
    27541 ccaccagcag ggtctttccc ttacgggaca gccaggaggc caaatgaacc gccagggtag
    27601 tcttgccgca cccccccttg tggtttgcca ccgccaggac gcgcgccacg ctcaaggcca
    27661 ccgctcgggc atcgtcagca ccagagccac ctctcctcct tcgaggaacg ccacccgtcg
    27721 cacgttccca aaacgaaatc cctccggcac gaggcgcgtc ggaaattcgc cccgcttctc
    27781 ccaacgatcc acccccagca ccgcccgccc cccggggctt cgcaccacca ccgaggcgtc
    27841 ggagggccgg agggcggacc tgagggcact ccggggaaag gaccacacct gccgggcggg
    27901 gaaaaagggc agcgcctctc ctcgaaaaag gacccacccc ttcgaggccg gagccaggcg
    27961 ccccacggcc tccacgtcct ccagggacaa gaggcaggga attccttcca cccgcacgag
    28021 cagggcggcc acgcttccct cccccgggga gggatgacgc ccctcggagg gacgctcggg
    28081 cccgggcaaa ggagagccct ccccttcggg gaggagggtg ccccgggaat ccgggctccg
    28141 ggacaggcac cgcagctcct ccaccaggcg ccgcaacgcc gccatcccgc gctccaggcg
    28201 ttttttccgg tctccttcga gatctttcag ggatcgttcc gtgccctcca ggtcgtgggc
    28261 caaaatctcc cccttatgga ggacctccgc caggggttga gagggggcca ccgccaaaag
    28321 cagggcgaat cccgccttgg cgctgtgaag aaagcgccgc agggcttggg gagcctggga
    28381 ctccaggagg agatcctccg gggccccgcc gtcaaagtcg tcgaggaaat cctccaccat
    28441 ccgggcgaac tccgcctggt ccgagcgagg aatcacgtcg ctcccctccc gaacaagggc
    28501 ttctggtaaa gcaccatgcc ccgagttcgc ttcacgcgga atcggctgga catgcggctc
    28561 acgggctccg cgtgccccaa aaagatgtac cccccggggc gaagatgatg gaagagctgc
    28621 tccaccatcc gctgtcgcgc ctcgtcgtca aagtagatca acacgttccg gcagaacacg
    28681 acgtcgaaat cggggcccag ctgccccatg tcctggggat ccgccaggtt ccccaggaaa
    28741 aagctcaccg gacgcttagt ttcgtcccgg aggacaaagt gctcccccac ccggtgaaag
    28801 tgccgatcca ggtaggcctt cggcacgtct ttcaccgccc ggcggccgta ccgtccccgc
    28861 cgagcccgtt ccaggacgct gtcgttgatg tccacggcca ccacctcgaa gggaaggccc
    28921 tcttcctcca gcatttccct caggatgatg gccagggtgt agggttcttc ccccgtggcg
    28981 catccggcgg aaagcacccg gagcccccgg cgcccccctc tccggcaccc ggcgacgagc
    29041 tccggcaggc aatcctcggc gaagctctgt agctggggaa aatccctgaa aaagtatgtt
    29101 tcgttcaccg tcacccggtt tattaggacc tgaaactcct ttctctgggg atcgaagaaa
    29161 cggaggtggt tgataaaacg ctcctcacga tcgatcccca gcacccccat gcgctcccgg
    29221 acgtggcgaa agacctgcat cctcttctga tcccccagaa agattcccgt gtgccggtgc
    29281 acgaaactcc ggatctcctc aaaacaccga aagtcctccg ggggctttcg agggcctccg
    29341 acgccgggct ctcccccccg gggtcccgga gggcgcggga agtccccgga gatcctttcc
    29401 ccggagggat acccccggcc ttctcttgga tccttctctc ccatggcgcc ccatcctccg
    29461 tcacaggagg ccccccgggg agtccccttc ggactcttgg gaaaatgcgg ggctggattt
    29521 tatcgctttt atctcttcca tcagttctcc cagggcccta tccagcgagg gccagagggc
    29581 cttcgctccc tccaggtccc cccccttgca agacgcctcc aggcgctccc ccagcgcggt
    29641 gagggcggcc acgccgaagt aggagacgct cccccggagt ttgtgcaccg ccttctgggc
    29701 ttccgccagg gcgccgcgcc ccagggcgtc ccctagggcc tgccccatgc ccggagcccg
    29761 gtccaggaac tcttggatca cgtccttcag gatgccccgg tctcctccca ctatgcgctg
    29821 tagccgggag aggtccactc cgccccgggg gagatccaaa ggttccggga cctcccttga
    29881 ctcctggccc agcacctctg caatggtgcg aaagagctct tcgggggaga tgggtttgga
    29941 cacgtagcca tccatgcccg cctccaggca tcgctcccgg tctcctttca tggtgtaggc
    30001 cgtcatggcg atgatgggca cccgttcccc ctctttttcc tcctggcgga tcagcgccgt
    30061 ggcctccagg ccgtccatct cgggcatctg cacgtccatg aggatcaggt cgtagttccg
    30121 gaagcgagag cgccgcaggg cctcgtggcc gttttccgcc ccgtccacgc tccatccctt
    30181 gcgctccagg aggatgcgcg ccaacttact gttcatgggg ttgtcttccg ccagaagtac
    30241 ccgaaggcgc tcgggcagcc ccctcaccgg ctccctatcc cgactctcgg agcgggagat
    30301 ggcactcccc tcgggagcca cgcccaggcg cacagtgaag gaaaaggtgc tcccttcccc
    30361 ctcccggctc tccacgccaa tggcgccccc catggcctcc accaggcgtt tcgatatgaa
    30421 aaggcccagt cccgtcccct gacgcttccg ggccatggcc ccctccaccc ggcaaaagct
    30481 ctggaagagg tccggcaggc gagaggcggg gatccccacc cccgtatccc gcaccaccac
    30541 cgtcaattcc agggatttcc ttgccgaaag gggttccgac cctccgggat cccctcggga
    30601 agccttcgcc tcttcccaga gggacacccc tttagggcgt cgcgccgtca cggtgaggga
    30661 caccccccct tggtcggtgt acttgatggc gttgctcacc aggtttacca ccacctggcg
    30721 aaagcggatc tcatccccca ggaccacggc ggggacgtcg ggggccacga ccaggtccag
    30781 gaatagcccc ttgccccggg ccaggggttc catggggcgc agggccactt ccagggattg
    30841 cctcggagag aagaggtccc agtccagcac caggcggtgg gcctcgatct ttgaaaggtc
    30901 taagacatcg ttgacgatct ccaggaggga ctgccccgag atccgcacgt tctccaagag
    30961 gtcgcgatgc tcctggggga cgtccatggc aagcatcagg tcggtcatgc ccaggatggc
    31021 gttcaggggc gtccggatct cgtgactcat gctcgccagg aaatcaccct tggaacggct
    31081 cgccgtctcc gccgcttcca aagaggccct tagatcctcc tcttcccttt tacgccccga
    31141 gatgtcgacc tggatccccg cgatttcggc ttcttcgggg cccctagggc acaagccctt
    31201 gaccacgagg tagtaccgcc caaggtagcg ggattcctcc ggagaaggct ccgcctccga
    31261 gaggagtccc gggggatcgc cgggggtctg ggcggcggaa aactcgaact gccgtacccc
    31321 cccgtcgttc cggagcgcca tgtcctgcca taccatctcc gatgcgagct cctggggaaa
    31381 gattccccag tccgtcttgc ccaaaagctc gctccggggg caacccgcga aggccgcgaa
    31441 ggcctcgttg cactccacgt agcgccccgc gccgtctttg agaaagatcg ccgccggcag
    31501 ggcgtccagc caccggcgaa gccgatcgca ttggtcgtcc gccccagcat acgtcatcgc
    31561 ttcttccccg tggcccaggg gtcggttttc ctctggcggc actcctatcc cctctttcgc
    31621 gcccgtcgcc gagactccgt ctcctccgaa gcccttaaaa catcgcccct ttaggaggtc
    31681 aattgggcaa tggcccgggc cactttttcg tcctcaaagg gcttgacgat gaagtcgctg
    31741 gcccccgcca tcatggcatc catgacgatc tcctggcgcc ccatggcggt gatcatcacc
    31801 accgccacct cgggacactg ttccttcatg agttttaggg ccgccaggcc gtccatgtcg
    31861 ggcatgttaa cgtccaaagt caccagatcg gggcgcagca gggcgcactt ttccactccc
    31921 tcctggccgt tggtcgcctc ccccaccacc acatgtccgc ccttttccaa gatcgccttt
    31981 agcttgaacc tcatgaacgc ggtgtcgtcc accaccagaa tcctcttgcc cacggccact
    32041 cacccctcgg tctcctatct tttccgcacc ccccctgccc ctacagggat gggggaaggc
    32101 ccactccccc gtcttccgcc cttcccgaag cccgcttcca cccctcctgg ggaggaaaga
    32161 tctcccgcat ctcttcgtcc cacccgaagg ggataagaca ccaggtccat tcccacccca
    32221 cgaccgtcat tcggaccatc tcctcctgcc ctgggttgag gggacctctc cccgggcgca
    32281 cgatgagttc tccgtccacc ccctgggctt ccaggcgctc cagctccagg cgcagctctt
    32341 ggcgcaccat ggcatccaca tccaaaaagg gcccccgacg ccacgcccgg ctctccatga
    32401 gggacgccag ccggggcgcc accagggggg ccaaaaggga aaagagctct tccgcctcct
    32461 ccgagtggag tggcacgcga tccagggatg atacggcgat ggtaccccaa caccggcgcc
    32521 ccgaccagag gggcgtgagc aacatggggc ggccggagag gagggtcacc acccggggaa
    32581 cttccatctt ggaggcccac gccaagagtt ccgcccgtcc cgaattggaa tctccccgga
    32641 ggatgaggta ggggggttgg gctcccaggg ccaggcgaag ggaggactcc acgctctcgc
    32701 aaagccgatc caaatcgtta atttcctgga ctttctccgc gatgtcccgg aggagacgca
    32761 tgaaaagcag ccgccggtcc agggcgtcgc gctgatcggt gacgcgctcc aggagttggc
    32821 agcgctccaa tcccaccacc accgatcccg ccagggtctt cagcgccatg gggtctccgt
    32881 agtccttgcc ggtcactgcg ggagccagcc ccaccacgcc gtagaactta tcctggtccc
    32941 ccatcaggag gatcctatcc agcccctgac gttgacagat cttcttcagc cccggcatgt
    33001 gggtttcgca gaagaaattc atctccgccc ccgagagcaa tccttcccga gggagggtgc
    33061 atccctccaa gatcccccga tggaccacct cttcgggggg acgaccattt cctgccggaa
    33121 ccccgtggag cacgtaggcc cccgcctggg gtttccagac caccagctcc agacgggatg
    33181 tttgagacat ctccttcagg atgtcgctgg ctgcgtccag caggtcgtga cggctttgct
    33241 ggcgcatgag atgtagcgtg gaggagtgga tcccttccag ggcgaaggcc ttggtggaaa
    33301 cctccgcaat ttgccgccgg ttttcttcgt gcagagccga tatttccagg gccttagaag
    33361 ccacgtggac gaagagggcc aggaagttca tatcctcttc cgaaggggcc ctgtcccatc
    33421 ccatgagggc aaaaagtctg tgggggcccc ccatcaaggg caggaccacg tggatgccca
    33481 gggcgcgcag ggccgcgagg ttggcgtctc cgagcacgcc ctcgtgctct tcatccacca
    33541 tgaggggcga tgggaggatc cgggtttctc ggaaaattcc ttggcgggcg ggctcatagc
    33601 gaggctcccc ccagaggtga cgccactctt tcccccgatc ttccagcacc gccaagcgcc
    33661 gggagaaaac cccctctcga aggacgtccc ccaggaaggt cgcgaagtat tccacgggca
    33721 tgggctcgaa gatggaggcg atggcgttcc gggcggccat gagggtgtag gaaaggcggg
    33781 ataaacggct ttccagggga accacctttt ggtgatcatg ccagagcccc acgaggcggg
    33841 caaaggctcc cagatcgttt cgcagctcca gggacagggg ggccgaggtc aaaaggtagt
    33901 agcaccactg atcccccgcc aggcgcgccg cctcctccca cccttcgccg aaggggtctg
    33961 cctcctcggg ggagagcgaa accactcggg caccgccggg ggccaagggg ttttcccggg
    34021 aaaaggctct ctccagaacc tgggatcctt ccgaaaagct gaaaaccggg gggacgaaac
    34081 gcaccgccgt caccaccgcc agtgacctcc ccgtctcctg gagcaccaag gccctcccga
    34141 gctccatccc cagccgctct tccaggaggg agaagacgcc cccgtcgagc acaaaggaca
    34201 tgcgatcgca gaattcccgc aacatcaggc tccccccgat tccacggcca ccacccggtc
    34261 ctcgttcgcg tcgtaaaaca gggtgacgcg gctcatcgcg ttctgagtta cgctcgaaat
    34321 cccccggagg cgtaagttta ccccgcagga acaggatccc cgcaggccaa aggtggcgtc
    34381 ccccttcatc ttgcgcaaaa aatcccccag ctcggcgaaa cgttcttccc gatccgccag
    34441 ggcgtcggct ttttctatgt agcccctcag aaggtcccgg atccccgggg tccccggccc
    34501 caacgcccgc atctccgccg ctagggcaga caggtggtcc tttagggcct cgatctcctt
    34561 ctctagggcc tggtgatctc taaagagatc cctcctaggc accccgggga tgtcgatgcg
    34621 agtgtcccct tcgcggaagg atcgtacgtc cttggcgtgg acatgccccc gggcgcggat
    34681 ctccgaagcg gtgatgaccc ccttaccccc ctccaccagc acgcccttac gggcgttcac
    34741 ggtggacctc aggatgtact cgttcaccat gagggtctcg cattctacct ctgcctcctg
    34801 gatgaaccgg gccatcacgt cccctcccgc ccgcaccacc gcccgtcctc gcccctggat
    34861 gccgtagcgc accgagacct ttccctggcg gctcgtcacg gcggcatcct ccacggagcc
    34921 caccacctcc acgtcgcccc ccgcctccac ggcaaagccg ccccgcacgg aacctttcac
    34981 cagcacgggg ccgtcgtaag agacgttgcc cgtggccgaa cccacgtccc ccgaaatcac
    35041 cagcactcgc tccagcagaa tcctggaccc cttccatcgg agggccccgc tgtatttcgc
    35101 caccaactca ccgttctccg atagataaag gccctcgccg aagtccaagg gcgcgggacg
    35161 ccccttccgg ggaggcacct cctctcctcg caccgtacgc cccgctctcc ccttcgtggg
    35221 gggaaagcgc atcgccacca ccgcgccctc ctgtaccggc acgatgagat ctagatgata
    35281 atgatccacg ttccccgagg catcctccac ggggcgtccc gtgggaggag agaagaaaaa
    35341 ctccactcgc ccatcttctc cgggcccggg gggcacccct cgggccacca cggcgcacac
    35401 ggcttcgcct gcggtggcgc gggcccacgc ccgatccagg gcctcgtgat ccacgccgaa
    35461 gaccacccct tcgcggtcca ggagctcccg gagacctcct ccgccgccct ggagggggta
    35521 aaatcccgga gaaaggtaaa gacggcaagt catacccgcc tcgtccacca ccacccgagg
    35581 cgaagcgcct tcgccccctt cttcttccac ccccacccga tggctcccaa ggaccgacgc
    35641 cgccctaaag acctcttccc gggcgatgcg aacctccggg gccacactcc ggagggcttc
    35701 caaaacctcc ataggtgcca gggggaggcg gcgcacctcg atccacacct cctgcgccgt
    35761 ccggcgcagg gcaaagccgg agccgacgtg gatccacgcc cccctttccg gaattttttc
    35821 ttccccatcg acttccggcg tcgtcatctc catccctcct ctcggatcaa ggagcgccaa
    35881 gatatatttt ttaataaaat aaccccaaag tcccccgaag gggctcacgc ccctccgggg
    35941 gccgataatc ccggaagaaa cgcccccgcc gccccggcct aggcgccggg agattcccgc
    36001 cgcctgaccg ccagagccac ttccaagagc ttggccactt gacggctaaa ggtcgccggg
    36061 tcgggcagct ccgtcccttc ggcgagccgg gcctgtccca gaagcacctg ggcaaactcc
    36121 tcgatccgag ggtcggcggc gtcggcggca tggagatccc ggagcccccg gagcacgggg
    36181 tgaccggggt ttatttccag cacccgcttc accgggggca ggtcgcgtcc catatggcgc
    36241 aggagcttct ccagctgggg cgtcatgtcg tggacctctc ccaccaggca ggcaggagag
    36301 gaggtgaggc gctcggacac ccgcacttct ttcacgtcct tgtccaaaa
//
LOCUS       DAOOER010000015        35279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_1340_length_35279_cov_8.764450, whole genome shotgun sequence.
ACCESSION   DAOOER010000015 DAOOER010000000
VERSION     DAOOER010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 35279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35279
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1340_length_35279_cov_8.764450"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..1179
                     /gene="serC"
                     /locus_tag="PL162_03520"
     CDS             94..1179
                     /gene="serC"
                     /locus_tag="PL162_03520"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HQF91132.1"
                     /translation="MSRKYNFNAGPSTLPLSVLEELQQDLVDYKGMGLSLLEDSHRGP
                     TYEAVHHEAVALFRELLGIPESHKVLFLGGGATLQFAMVPMNFLAAGKSAAYVNSGAW
                     GKKAIEDAKKLGQVQIVFDGKESNFTTLPDPASVKVDPESAYLHFTSNETIGGLQWKE
                     WPDAGKVPLICDMSSDIFSRAVPVKKFAYIYAGAQKNLGPAGATVAILREDMLGRIPD
                     TLPAYLNYKNHVENDSLYNTPPVFAIWTIKLVLERMKKLGGLAAAADHNARKAAVLYE
                     AIAKSGGFYSCPVDPKYRSDMNVVFRLPSEDLEKKFVKEAEVLGMIGLKGHRSVGGCR
                     ASLYNAMPLEGSQALAAFMKDFAAKNG"
     gene            1190..2461
                     /locus_tag="PL162_03525"
     CDS             1190..2461
                     /locus_tag="PL162_03525"
                     /inference="COORDINATES: protein motif:HMM:NF018004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HQF91133.1"
                     /translation="MSETRERLAKLGVGIPEILLPTPGTDLTKWAVVACDQFSSERSY
                     WEETDRLVGDAPSTLRLILPECYLEDGDPAPRIEAIDAAMEAYLAQGHLRPLAPGFIL
                     LDRSTPFVTSRKGMMLAVDLERYSFEPGSTALIRPTEGTILKRLPPRMAIRRGAALDL
                     PHILLLVDDPEHTLIEPLFAARKALEQVYDFDLIQGGGHITGWHVPESHMGAVASALE
                     GLAQGGMLFAVGDGNHSLAAAKQLWEEKKAQGAPADHPARWALVEVENLHDDGVLFHP
                     IHRALFHVDISHLKHAMGQRLGGSFLPGEVAPRQEGSHHSLGYVFPSEKGNFVFDRPG
                     NRMTVEFFQEFLDDYLAGHQGVSIDYIHGEGALEALARTPGSAGFFLPPVAEETFFSR
                     IVTVGVYPRKTFSIGEAAEKRYYLEARRIKP"
     gene            complement(2522..3493)
                     /locus_tag="PL162_03530"
     CDS             complement(2522..3493)
                     /locus_tag="PL162_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91134.1"
                     /translation="MRRSKWWLFVVLTVCWGSGAAFGASRLPVTVRGDAIYIGDSVVI
                     SFQRTLRVPDDGRRWDLPPGLGRFPIRRVDDYASRVPASWRRQGGVIIPMYQREALWI
                     DFRSSQGPAAVKVAAGMINALTGDSWSDRLSPRQDYLVVPDQPWLDGFNTGRGAVRQF
                     VAMPLGSGGTVEGQLTGQERYGGIQIMVYPAKEFWRDRFGEQGKGSSLGSAQGSPGMG
                     LGEGGKISQRIAEDSYGYDTWDQRRPGRIYVHIVNGRLWRAITGEPLPPTPIRFETYQ
                     QYHFPWSDIFDEGDEGWGSGAPAPKPKGWTYDRVPVDDDDLVRDGDW"
     gene            4025..4411
                     /locus_tag="PL162_03535"
     CDS             4025..4411
                     /locus_tag="PL162_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQF91135.1"
                     /translation="MRWLKTYADRCIGCGKCAEVCSTTYFKENNGACSRIQVTDPGAV
                     RTLSVCNQCGTCIAVCPTQAISRDKNGVVQIDKKKCTSCLMCVGFCPTGNMMAYAPKQ
                     SEPFKCIACGLCARSCPAQALELKND"
     gene            4440..6617
                     /locus_tag="PL162_03540"
     CDS             4440..6617
                     /locus_tag="PL162_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HQF91136.1"
                     /translation="MTHEKIAPMKVLQEWSYVRSPIDRGYTRKALYVNVGKPDMQIRE
                     IPQETIDAFTGGRGFGLALLWNAVTEDTKWNDPENELVISGGPLCGITQYPGAGKCYT
                     VFLSPLSEQTYDSNSGGYWGPLTKFSGFDAVEIAGKADRDVVLFVNGDEGKIQILEST
                     LPESNAYTVTEALHDYLAADEGDKVNISVVSAGKAAETTLWGCLNMSFYDPRRKTARL
                     KQAGRGGGGTVLRDKKIVALVVKYSSVKGTENNPVDLSTLQKVGVKLHKEIRDLDNVQ
                     CRMRKVGTPHLNEIMDEYHLLPTNNYKFGQNKKDIGKIDSYTYEKIFTQGLPDGCWYG
                     CSLACAKAVDHYEIQTGPCKGRSVTVDGPEYETVASLGSNIGVYDPLWTVEANFYADH
                     YGVDTISLGTGLAFVCECYELGYLDKAKTNGLDLSFGSKDDLMELIHRMAEGTDDFAR
                     AVGLGIKKMKKVFAERYGADGAVLEKIGMEAQGIETSEYVPKESVAQWGGYYLTLKGP
                     QHDEAWLIFMDMVNKQLPTFEDKAEALHYFPNFRTWFSLVGLCKLPWNDIEPADNHVR
                     YRPQDAARVPEHVQNYVDIFNAVTGKQIVKEDLITMAERVYNFQRLFQVRMKHGNAEH
                     TIPARAMGPVFPDEWDARKEYYDDKLREAGIAPEGLSVEEKIEGLRGHRLEQWADLKD
                     AVYKRRGWNKKGIPTEETLKKLGIAFPEAVAVAKKYCRPEDEF"
     gene            6624..6821
                     /gene="thiS"
                     /locus_tag="PL162_03545"
     CDS             6624..6821
                     /gene="thiS"
                     /locus_tag="PL162_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HQF91137.1"
                     /translation="MIVVNGEELPWHPGMTVQQVIDAKRYTFRMLAVWVNDVPVLRER
                     FATHTVADRDRIQVLHMISGG"
     gene            complement(6928..7353)
                     /locus_tag="PL162_03550"
     CDS             complement(6928..7353)
                     /locus_tag="PL162_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91138.1"
                     /translation="MSNQSCAVNSQQSLEMSSQARSGAQGGVAEGGAAKKWWLVQLGF
                     FVLMVLVFNVMWTQMGHRVEGVRDTIKSYEQGLEALNKDVAELQQQVVVVMDAGSRHD
                     GALRASIQNQRNAVQEQLRLLQQQLQELDNQETQLGGGQ"
     gene            complement(7498..7668)
                     /locus_tag="PL162_03555"
     CDS             complement(7498..7668)
                     /locus_tag="PL162_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91139.1"
                     /translation="MGAIDGVLAADKARTDVQIQTAVAAKIMKSQDNLAQDLISKLFA
                     SVGIGGQLDLRA"
     gene            complement(7752..8324)
                     /locus_tag="PL162_03560"
     CDS             complement(7752..8324)
                     /locus_tag="PL162_03560"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQF91140.1"
                     /translation="MWAKHLKEDALFFRKFLQSPGTVGSVVPSSRFLVRQMLSPVDWK
                     RAVFVAELGAGTGILTRDIVRRRAPRARVLVFELDDAMRENLKDEFRDCDIHRDASQL
                     KEIMDRERIPAFDCIISSLPFAVFPQELRQTLLENIQACLAPSGVFVAYQYSLQMRSQ
                     LAQVFDDISLRFVPLNVPPAVVYRCRWKDR"
     gene            8697..10115
                     /locus_tag="PL162_03565"
     CDS             8697..10115
                     /locus_tag="PL162_03565"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQF91141.1"
                     /translation="MERRGSFRKALGILGAALVLGWSGATAAVEFDVAADTPLVVRSG
                     EGKVIVRALVRPEAGRRQERAPLAVALVLDKSGSMEADGKLEHAKIGALEALQVLDPR
                     DMVTVVTYDSSVRVAVRPREVGQGARDSRLTRAIRRLSPQGNTALYEGVLVAMEELLP
                     FAEEGYIPRLVLLSDGQANVGPTSRGEIQALGRQCAQEGITLTTIGLGLDYNEDLMTA
                     LASSSGGNAYFVHEPSTLPEIFGRDMKDAVSIVARRVRIKLRCPEGVRPVRSLGRTAT
                     AEEENSLEVTIENLYGSGEKYALFELEVSPDEEALSRDIATVELSYVDPATGKELREE
                     RRLAVSFTDSEGEASRARREEIVSQTALALNAEAREKVLDLVDEGNAQEARKLLHQRQ
                     QALAAMPAAPGSGVAPSWLSDEIGALGILEESIASSGTLSSYARKQALNDAYVQKNQQ
                     VPATTSPDLSPDISPDISPDQR"
     gene            10319..12268
                     /locus_tag="PL162_03570"
     CDS             10319..12268
                     /locus_tag="PL162_03570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQF91142.1"
                     /translation="MKLFLKHPQTLRRPLALLFLLGVLLPSVALSVLALRAASREALF
                     VERRLEVALLAEVGDAARQVERALEDIREELSQELKDLPLTPRDLARWGSRQELAAVP
                     FLLSQGVLSLPGGSAAEKKAFLQDFGPFLRGEGALPVYDRAADVYRRRVPLPESPCPP
                     EAISRVVGRGAAPPLPDILSAIPPEGDAGAPPAPREGGVQSWVPLAALREGPFVSPED
                     SSDRDEPSPGSIAGIRSLVRNVIPQARPEEWASAGVPSTFVARQSTLATLRRQGSSGF
                     IPRLSDGGLVLLFWSSGENGTVAGALIRAEPLRRRLLGVLPEPRTEDRILAVLDDQGN
                     PLENEGGEPSLDWRRPFVAREISPSLPYWEVGAYLTDPEMVASQAQMVRGGVWLLVAT
                     LLVAITAGGTLILRSLSAEMRLAGQKTTFVANVSHELKTPLTSIRLFAELLLQKRQPN
                     EGRREEYLQIMVAEAERLSRLVDNVLTFSRQGSGQNSLRHDPLDLAALAAAVGDQLRP
                     HLEREGFALVVDAPEPLPVRGDEEGLRQVLVNLLSNAEKYSGDRREVELGAFRREDRG
                     VVEVRDRGLGVIPAHAERIFQEFFRSDDSLTAPRRGTGLGLPIARHVARGHGGDVTYA
                     PRRGGGSVFSLEIPLDLPKEEVKNP"
     gene            12265..12966
                     /locus_tag="PL162_03575"
     CDS             12265..12966
                     /locus_tag="PL162_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQF91143.1"
                     /translation="MNERILVVEDDGAILTGLEDLLSSEGFSVITARDGDRAVELWHR
                     DRPDLILLDLMIPRRSGYDVCRAVRREDRDTPIVMLTAKGQEVDKVLGLELGADDYVV
                     KPFGVAELLARIRAALRRGKRPLTPERPSGRHVFGAVEIDLEGMRGSLQGEPFDLTPL
                     EGALLAYFLAHEGKVLDRHTLLESVWGISTGVTTRTVDQHVVRLRQKIEENPGTPRHL
                     QTVHGMGYRFVRNPL"
     gene            complement(12963..14270)
                     /locus_tag="PL162_03580"
     CDS             complement(12963..14270)
                     /locus_tag="PL162_03580"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91144.1"
                     /translation="MKSDFSGHLPGFDVLRRIEAQGFPAFLVGGVVRDLLRGRAGEDV
                     DIVTLAPPEVLRRLFAGAPEVGKAPLGVWVVPREGGVAEVASCHPHSLEEDLARRDFT
                     VNALALGVDGQLVDRSGGMEDLFRRRLRWNGDPRDRLRQDPLRALRLARFAATLPGWI
                     LDPGALAPCAPFAGALGEIPGERVGREMARGLRGDPALFLNLLERGGMLGAVLPELGA
                     LRDAREPGEDGDLLSHTLRVLGGVASLSPELRDRAGALWHDVGRGVELRGGDPEALSM
                     APGILGARRAARRMALWRWPRGLGRSVEALVRHHSLGADPPSPEGLGRLARRHGTAWL
                     EGLLTLFWAHGQEGGSFALWWEAHRREALALRGRWDRWGGGRVSGDAIRALTGLSPGP
                     GVGALKARLEEAVDRGEVTSEEGARIWLRRFSSEGVKEGPGTA"
     gene            complement(14337..15518)
                     /locus_tag="PL162_03585"
     CDS             complement(14337..15518)
                     /locus_tag="PL162_03585"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HQF91145.1"
                     /translation="MKSRKGVTGLEKKTYVTTPRLVMGCGTFAQAGEEMARLGASRVF
                     VLTGGHAVREGKAGALEKALRDRGMESALFDGIEPDPSCDTLERALEAARKFEADAVV
                     GLGGGSPLDMAKAVSVLLTNEGPLRSFFGVDLLYRRGLPKLLIPTTAGTGTEVTAVAI
                     FSDHKEKLKMGIVSPFMVPDVAILDPELTLSVPPLVTAYTGMDALVHAMEAYTSVNAT
                     SLSDMYAERALALLFPHIRAAYADGRNLAARSAMMEGSMLAGKAFAIAGTTATHAFAY
                     PIGAEFPIPHGMANALMVVEVFQFNLLGNLLKFARIADLAGEAVEGLPLRDAAQGLVE
                     SLRRLVEDLRLPTRLRDFGVKKADIPRLAAGVLKVTRIMANNPRRLELEDAEEIYRRV
                     L"
     gene            15801..17486
                     /locus_tag="PL162_03590"
     CDS             15801..17486
                     /locus_tag="PL162_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQF91146.1"
                     /translation="MDTPPMVPIGLRAKLHLVAFGIGEALVRVTMALLLIGATAAGIS
                     AEQSTPHPASYFEPAPCPCDVPPELQSRVHCGYLSVPEDRQKTSGKVLRLAVAVIHSS
                     HPRPAPDPVVYLEGGPGGGTLAHLADWVDAPLLRDRDLILFDQRGSGFSEPDLWCSEL
                     EEAEYSMLDKVVSPDRRQEAFSRAAQACASRLERAGVDLSCYNTAAIAADVEDLRRAL
                     GYARWNLYGVSYGTRVTLVSLRDFPQGIRSVVMDSCVPLEVQEYEEAPRNALRAFDRL
                     FQACREDPSCHRVFPDLEGTFAHLVERFNADPLPLEVTVVGAEGEEEVISMAFSGNDL
                     LGTLFQLLYDHQAIPYIPLLIERLRRREYTPLGAIASDLLVGGLGDGLYLCVECRDEA
                     LFNWSSDVAENLNLLPPGRRYNPYPYDLELCAVWPSGNAPLRESFPVASDLPALVMAG
                     GFDPVTPPEWSRMVARTLPNAFYVEFPSLGHGVSTTSCGGEAVAAFFRNPLRPPAPGC
                     LMRAAPLSFALEQKGQESPYGIADPESPPPSQGLLEALWKFLRQSMEIPSEEP"
     gene            complement(17508..18521)
                     /locus_tag="PL162_03595"
     CDS             complement(17508..18521)
                     /locus_tag="PL162_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HQF91147.1"
                     /translation="MRKATCLRVLGLRPAAGEDELREAYRAGVKKYHPDTPTGDPCRY
                     MVLQRAYTLLLRGGPYDDEFEDELARAGFDDGEGAFEGPPVTVSAFDFPEISPGEGPL
                     TPGIPRKRVSEKNPEEPSLKVPPGVRLDAGVVAGLVRGFLQEARVVLPPVDLVEVARK
                     QGLSVEMGALPRDYGRCNRLKDAAVHAGRVEWRGDRGHIVVEGLFNADKKWQRYVLAK
                     MMGYALLSPGRDRAEVLGEFSPPRDDGEEENAHRLAEELLLPEDLVSEHGGVVLGRLL
                     RSHKLDYDAFLLRMEELFLVPSPLARRRLAKYEARLRILLHMASRRPSRRGLFDLCED
                     ALY"
     gene            18826..19941
                     /locus_tag="PL162_03600"
     CDS             18826..19941
                     /locus_tag="PL162_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91148.1"
                     /translation="MRIQEGSILLLKLYDVADEINLEAIPPDIFAGKLPVKQRLVRVD
                     ANSIELVRPPVAVDLGPRAWETAGGRLVLDIAAHFFDMGVMGVAARIPVRDVTLEEFS
                     RLALALQQDPEVDRQLAWEVRHILEGVAPALEGPHSTLEFDEEFAFYIIQRADAPLSM
                     KHLESHPLLVSMLQGASRPVSPQQMELALKNSFSFHEDEDLVVLNYNNALVVDREDCG
                     DVLLLVEYAVVQVLEMRYYNDLLTKRLQELHDDADRKLSLMEALFSRQWSALSRRGMK
                     LLLETRLMTDHLTSAVQVTEDVYYAQIYSRALRIFDTQQWLAKVDEKLRSMKEACDLM
                     EHEIEFRRATILEWIVIALIAFEGIPIIRELARWLLR"
     gene            complement(20171..21139)
                     /locus_tag="PL162_03605"
     CDS             complement(20171..21139)
                     /locus_tag="PL162_03605"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQF91149.1"
                     /translation="MNRTRRTHFAAVFLCFLFLGVVLGSTASGAEKPSPAKEKPLPWT
                     EYVVGAGEDAEAVATKHHVKSQYLLWANDLSPQAPLSVGMVLLIPRSSSELPATLMEV
                     RARKAQARRSQEAKVAQRPSAPKPPQASSVTPSQGKTTDASTSDLEAMLAAMTPEQRK
                     LFGPKLPSKPSTEEEAVVVEGGRRSSRAGLIWPVVGRVSSGFGMRGGGKTKSGRVRRG
                     QFHNGIDIPMPKGTPILAAKGGVVVEAGPARGYGQRVVVDHGNGLRTLYAHGCKILVK
                     KGQRVVQGQRLALVGRTGRATCNHLHFVVYVNGKAKNPVEYLPKKP"
     gene            complement(21278..22456)
                     /locus_tag="PL162_03610"
     CDS             complement(21278..22456)
                     /locus_tag="PL162_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91150.1"
                     /translation="MAFEPKQVLAYERVDEFLSLSLTLGGKGPHCVIENLATGKKKLV
                     RLSWVESGKGLQIKTGKKELRSYDADTIFRASRSLVAQLLTLPLVRDRFRHKLNFFLG
                     ALHEPRSVLRERDLRVLSEDRRRGLWLACFPELGSPGVLRPCFPFQGEEERLLLSRGR
                     DHGAPWGFGFDLDPGERGLGPLKRHGMVAALVKVHPQRWFYPVVAAVAALLTGLSQGS
                     SGGEEEDTALSLLWRPLMAGHTLDPEEDLRLASSASRLLEGLRLYIRHFYRRKDVGHR
                     EILDGEEILKGEGYGRRERFEDGSGALGRHIYSVSRYVRDDGGGALICQVKGATYPPK
                     EDVVLTFGPGVYEGALRDDSCGGLEDGRFTTLNIFRMVQMHQWLKTVERALGDPATLP
                     "
     gene            complement(22506..23669)
                     /locus_tag="PL162_03615"
     CDS             complement(22506..23669)
                     /locus_tag="PL162_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91151.1"
                     /translation="MAQVGDLMLIPPFCSVPILLFVLALGSPVGEAALPLPLWGGVIL
                     AYGFCSTLYVLLTLRGVRRGRGALQLMAQGILLPPSALAAGAPGVVAWAGVALLLAGL
                     ALGGLEVVSSRCAEGSASGEESPPCLDTIAKVVGQLPLPVILSDTRGVVRGVSPAMVP
                     LLAEVPPPLEGKFVDSFMDIGAQEVLFQGKPWLQEQVPLMEEGAVLFLLSPGRSEEPT
                     EDRSSGRPSEGVPLIDPRTGLFNEAFVKPRCEEEIARDYRYRRWMTGVWLRLDLRGVV
                     SPTEERLVREDFAKLVRGMIRTCDLGFALRNGDVFLILPETPLGGAQALVRRVYDQAM
                     ALMVGFAKTGMKFQYEFKAGFYFYKGTEALHVHTLLESLQSSLKTFSPKEEME"
     gene            complement(23834..24307)
                     /locus_tag="PL162_03620"
     CDS             complement(23834..24307)
                     /locus_tag="PL162_03620"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HQF91152.1"
                     /translation="MTERAFDMKHALRYAIHSEYHAAAFYRQWAQETSGAAKEQIEGL
                     AEWEDQHRRSLSGYYARLFGEEVNLDPSFGVDPGLVVQADEFRNHYALVRIASTVYVT
                     EMRAAEMYDQMARHAADPEGAKIFSDIADMERGHMQEALAMYQRLREDQAGSLML"
     gene            complement(24481..24942)
                     /locus_tag="PL162_03625"
     CDS             complement(24481..24942)
                     /locus_tag="PL162_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91153.1"
                     /translation="MLYFNYASRMALFALAFALLYVRFILPVVAKARDALVPLPGEER
                     LGKVTQDVGEFLGMVIESYLRGGWAAWCAVTAVTYARLPETTGWYYIATAFIICETAL
                     AHVGRRDEGEGMMTILRSVIPMGAFAEFAFSPDHAELVYGWVGRLWPSNFF"
     gene            complement(24947..25603)
                     /locus_tag="PL162_03630"
     CDS             complement(24947..25603)
                     /locus_tag="PL162_03630"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HQF91154.1"
                     /translation="MLLEREREQLVLFGRRMVTAGLTRGTGGNLSVLRRDEGLWALTP
                     SGLDYFELRPEDMVVLDLEGRVREGSRAPSSEMALHREVYHRRPDAGAVVHTHSPFAT
                     TVACLGETIPAVHYLVAFAGLDVPCASYATFGTEELARGAAACLACRDATLLAHHGLL
                     ALGRSLERAFAVAEEIEFVAELWWRARCVGTPATLGEEEMVRVRKKFVHYGQGAEEER
                     "
     gene            25855..26691
                     /gene="panC"
                     /locus_tag="PL162_03635"
     CDS             25855..26691
                     /gene="panC"
                     /locus_tag="PL162_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HQF91155.1"
                     /translation="MRIVTTPAAAREALASMPKPVGLVPTMGFLHEGHLSLVRRCRGE
                     NATAVVSIFVNPLQFGPREDLSSYPRNLVRDQDLLERLETDLVFAPDASAMYPSDAST
                     FVEETRLSADLCGAARPGHFRGVCTVVLKLFHIVQPQRAYFGEKDFQQLQVIRRMVRD
                     LDVPVEVVGCPLVREADGLALSSRNVYLSPEDRLRARALSRSLAHARDLVARGERDAR
                     ALQREVEAFLRRDPALELDYVAVKKADTLEDADSLEGRILVALAARVGKTRLIDNSVL
                     EV"
     gene            26699..27067
                     /locus_tag="PL162_03640"
     CDS             26699..27067
                     /locus_tag="PL162_03640"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HQF91156.1"
                     /translation="MLLHMLKSKLHCAVVTEADPDYEGSIAIARDLMEAAGLLPGEKV
                     LVANVENAHRFETYVIPAPAGSGTIGLNGAAALLGSPGDRVIIMAWGIFSEEEARAHA
                     PTVVRLTRENGVEARFPMPR"
     gene            27177..28013
                     /gene="panB"
                     /locus_tag="PL162_03645"
     CDS             27177..28013
                     /gene="panB"
                     /locus_tag="PL162_03645"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HQF91157.1"
                     /translation="MERLTIPRLWEMKRQGEPIVMLTAYNVWQTRLVEAAGADMILVG
                     DSLGMVEHGYEGTVPVTLDMMILHCAAVMRSRKRTFVTGDMPFLACEVSDPEAVRSAG
                     TLLKESGVDSVKVEGGRRRAHTIAALTEAGIAVMGHIGLTPQSAVSLGGFRVQGKTTE
                     QARRLLDDARAVEDAGAFAVVLECVPAELAAIVTAQLSIPTIGIGAGLGCDGQVLVFH
                     DVLGLYGDFKPKFARRYAEGGTILTQGLESFVREVRQRAFPAPEHAFALDPRVREELE
                     KP"
     gene            28010..28927
                     /locus_tag="PL162_03650"
     CDS             28010..28927
                     /locus_tag="PL162_03650"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HQF91158.1"
                     /translation="MITIVGCGALGSLFAGTLIDAGFPVQGFSRPGAHLEACRRRGLL
                     VEYPDGSERTYHFPLEDSWERLEPSDLMLVLVKTHQTAAVAPHLPSALKPRGMVLTLQ
                     NGLGNAEILASSLSPGQLALGTCSYAAFRPAPGNVRWGGTGEIWLGPWDPTTDVSPLI
                     ASFQKAGLSAHFTPHPQRALWEKLVVNASLNPVTALAQCPNGEILRHDGLLNVARTLC
                     DEAVQVARAEGFPLEEETLWQRGLAILDRTKNNRSSMLQDVEARRRTEVDAISGAVLD
                     RGKKHRLPLPMTSCVTALIRALDAQGDRS"
     gene            complement(29022..29615)
                     /locus_tag="PL162_03655"
     CDS             complement(29022..29615)
                     /locus_tag="PL162_03655"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HQF91159.1"
                     /translation="MACKIVEAGAVAPEFVLRDQFDQDVALRDYRGKYVVLAFHPLAW
                     TPVCADQMRTLDRHRGLFDKFGAVCFGVSVDPGPTKKAWREHLGLKSLRLLSDFWPHG
                     AVSQAYGILREEGFSERALVVVDPEGTVIYSRIFPLGESPSLKSLAFFLKDITGSEVD
                     PNVEISQCLVDAQGGMVCMPDSRNVGLEDTRPGEMGG"
     gene            complement(29673..30539)
                     /locus_tag="PL162_03660"
     CDS             complement(29673..30539)
                     /locus_tag="PL162_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQF91160.1"
                     /translation="MKDPAIFDMENPGEISWCPGCGDFHILEALKKALAELDLEPHQV
                     VAVSGIGQAAKLPHYMRCHFFNGLHGRALSHATAIKVSNPELVVLAIGGDGDMYGEGG
                     NHFLHTIRRNPDIANFVHNNMVYGLTKGQASPTSPLGFRSPTSPEGTSNLPMNPLAVA
                     ISLGAAFVARAFSGDVDKTKEIMKRAILHRGYALVDILQPCVSFNKVNTFKWYRDNTY
                     YVGPEHNPRDPQAALALALQADRWPLGVLFVNPHPTFEETRSAYGDDARPLFRRPRDP
                     RGVREFLEAHYR"
     gene            complement(30536..32281)
                     /locus_tag="PL162_03665"
     CDS             complement(30536..32281)
                     /locus_tag="PL162_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HQF91161.1"
                     /translation="MANYPRANLGDVSLVLCGAAGQGVQTVEQILVKLAKAAGYHVFA
                     SREYMSRVRGGSNSTALRISSFPVRAPVDRIDVLLPLNRGVRSHILERIDGGTAILGD
                     PEELGDEFRRWEGQTVPVSFLSLAREVGQALFAGVVAVGVLAALLEIPREKLEEALRR
                     HFGGRGTDMADKNWAAGARGYAEGEALRRSGRLEMDLVADPAVEGAILVSGTEAVALG
                     ALAGGCDFCCGYPMSPATGVLQFLVAQARRFGVVTEQAEDEIAAINMAVGASYAGARP
                     LVSTSGGGLALMGEGISLAGITECPVVIHLAQRPGPATGMATRTEQGDLDLALYAGHG
                     EFPRAIFAPGTLEEAFFCTQRAFAQALKWQSPALVLTDQYLVDLSHDLPIPDLERHAT
                     GDYLIETKEGYRRYAYAEGGVSPRGVPGHGEGLVGADSHEHDEEGHVREDFALRQRMH
                     AKRMKKLEGLTRDALPPVLVGPEDHRCLVVVWGSTRPVGEEALARLGRKDTALLSFPQ
                     VYPLPPSTKDFLARAERVVAVEGNATGQFARHLFLATGRRPDASVRRWNGLAFSVEWV
                     AQELGKALDEGGEMA"
     gene            32520..33038
                     /locus_tag="PL162_03670"
     CDS             32520..33038
                     /locus_tag="PL162_03670"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrophenyl compound nitroreductase subunit ArsF
                     family protein"
                     /protein_id="HQF91162.1"
                     /translation="MDRRRALGVGLLVFSLVSFAVALRDTLRDFATPSPSGGAPASSG
                     RHLAIYLLYGNAKCPTCDDIMRLTEETLREDFAAPRERGEIVFSSANVEEAGNEWYVS
                     TFEIFSTSVVLALYDQGRLVTWKNPQEVWDLAADPAAFKAFLRREIQSMLSFSPQGPE
                     NPGAAASEDHPS"
     gene            33035..33715
                     /locus_tag="PL162_03675"
     CDS             33035..33715
                     /locus_tag="PL162_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91163.1"
                     /translation="MSAIVIAALGATWLGILSSVSPCPLAVNVAAISYLLRGGRTGRA
                     FAESAALYILGRSLIMALVGFLVVRGIFAVPELAMILQKSMNRFLGPLFVVVGVFVLD
                     LVPVRFPWTKKVGKVRFGVDRGGSFLMGVVFGLAPCPVTAALFFGALVPMAVEHASPV
                     LLPLLFGAGTALPVALAAGLAFFGVSVLAKALLSFATLDRWLTLANGAIFISLGLSYI
                     LTFVFPIL"
     gene            33734..34444
                     /locus_tag="PL162_03680"
     CDS             33734..34444
                     /locus_tag="PL162_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HQF91164.1"
                     /translation="MESTVATIPILGSLANSGAILAGGFLGVLLRSRFPERLSTIAFQ
                     VMGLFSLSLGVSMASKSANSLVLIFSIVAGAMAGELLRLEDHTDRMATKLKQRFAAHD
                     EGFSQAFMTTFLLFAMGSMGILGAIEEGLGKGPHLLYIKAMLDGAASMILAATLGPGV
                     LVAALPLGVYQGSIALLAGQLAGVLTPPVVNEISAVGGLMIMGIGLSLMGVLKIKTMN
                     LMPSIVVAAILGYAFCPV"
     gene            34600..34674
                     /locus_tag="PL162_03685"
     tRNA            34600..34674
                     /locus_tag="PL162_03685"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34632..34634,aa:Val,seq:tac)
     gene            34701..34777
                     /locus_tag="PL162_03690"
     tRNA            34701..34777
                     /locus_tag="PL162_03690"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34735..34737,aa:Asp,seq:gtc)
     gene            34805..34880
                     /locus_tag="PL162_03695"
     tRNA            34805..34880
                     /locus_tag="PL162_03695"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34838..34840,aa:Phe,seq:gaa)
     gene            34895..34969
                     /locus_tag="PL162_03700"
     tRNA            34895..34969
                     /locus_tag="PL162_03700"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34927..34929,aa:Gly,seq:gcc)
     gene            34992..35065
                     /locus_tag="PL162_03705"
     tRNA            34992..35065
                     /locus_tag="PL162_03705"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35024..35026,aa:Cys,seq:gca)
     gene            35160..35234
                     /locus_tag="PL162_03710"
     tRNA            35160..35234
                     /locus_tag="PL162_03710"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35192..35194,aa:Asn,seq:gtt)
BASE COUNT         6569 a        12000 c        10627 g         6083 t
ORIGIN      
        1 gccgcacatc ggaggcaaca gcaaggagaa cttgctccgc atcggcgacg aagtggtgga
       61 tattatcggt acaatgaaga aggagggaac actatgagcc gtaagtataa tttcaacgcc
      121 ggaccttcca cgctgccgct gagcgtgctg gaggagcttc agcaggacct ggtggactac
      181 aagggcatgg ggctttcgct cctcgaagac agccaccggg gccccaccta cgaggcggtg
      241 caccacgagg ccgtggcgct cttccgggag ctcctgggga tccccgagag ccataaggtg
      301 ctcttcctgg gagggggggc cacgctgcag tttgccatgg tccccatgaa cttccttgcc
      361 gccggcaaga gcgccgccta cgtcaactcc ggcgcctggg gcaagaaggc catcgaggac
      421 gccaagaaac tgggccaggt gcagatcgtc ttcgacggca aggagagcaa cttcaccacg
      481 ctccccgacc ccgcctcggt gaaggtcgat cccgaaagcg cctacctgca cttcacttcc
      541 aatgagacca tcggcggtct ccagtggaag gagtggcccg atgccggaaa ggtgcccctg
      601 atctgcgaca tgtccagcga catcttcagc cgggccgtgc cggtgaagaa attcgcttac
      661 atctacgccg gggcccagaa gaacctgggg cccgccggag ccaccgtggc catcctccgg
      721 gaggacatgc tgggacgcat tcccgatacc ctacccgcat acctcaacta caaaaaccac
      781 gtggaaaacg actcccttta taacacaccg ccggtttttg ccatctggac catcaagctg
      841 gtgctggagc gcatgaaaaa gctcggcggc ctcgccgccg cagcggacca caatgcccgc
      901 aaagccgccg tgctctacga ggccatcgcc aaaagcggcg ggttttacag ctgccctgtg
      961 gatcccaagt accgttccga catgaacgtg gtcttccgcc tcccctccga ggatctggaa
     1021 aagaaattcg tcaaagaagc cgaggtcctg ggcatgatcg gccttaaggg acaccgcagc
     1081 gtgggcgggt gccgggcgtc gctctataac gccatgcccc tggaaggaag ccaggccctg
     1141 gcggctttca tgaaagactt tgccgcgaag aatggatagg acctctacct tgtcggagac
     1201 cagagaacgc ctcgccaagc tgggagtcgg catccccgaa attctcctcc ccacccccgg
     1261 cacagatctg accaaatggg ccgtggtggc ctgcgaccag ttttcctccg agcgatccta
     1321 ctgggaggag accgaccgcc tggtgggaga cgccccctcc acgctgcgcc tcatcctgcc
     1381 cgagtgctac ctggaagacg gcgaccccgc cccgcgtatc gaggccatag acgccgccat
     1441 ggaggcctat ctcgcccagg ggcacctgcg ccccctggcc ccgggcttca tcctgctgga
     1501 ccggagcacg ccctttgtga cgtctcgcaa ggggatgatg ttggcggtgg acctggaacg
     1561 ctatagcttc gaaccgggaa gcaccgccct catccgcccc acggagggga ccatcctcaa
     1621 gcgcctgcct ccccggatgg ccatccggcg cggcgccgcc ctggacctgc cccacatcct
     1681 gctcttggtg gacgacccgg aacacaccct catagagccc ctcttcgcgg cccgaaaggc
     1741 cctggagcag gtctatgact tcgatctgat ccagggggga gggcacatca ccggatggca
     1801 cgtgccggag agccacatgg gcgccgtcgc ctccgccctg gagggcctcg cccaaggggg
     1861 catgctattc gccgtggggg acggcaatca ctccctggcg gcggccaagc aactatggga
     1921 agagaaaaag gcccagggag cgcctgcgga ccaccccgcc cggtgggccc tggtggaggt
     1981 ggaaaacctc cacgacgacg gagtgctctt tcaccccatc caccgcgccc tcttccacgt
     2041 ggatatctcc cacttgaagc acgccatggg gcaacgcctg gggggctcct tcctcccggg
     2101 agaggtggcc ccgcgccagg agggatccca tcactccttg ggatacgtct tcccctcgga
     2161 aaaggggaat ttcgtcttcg atcgtccggg aaaccgcatg acggtggaat ttttccagga
     2221 atttctcgac gactatctgg ccggacacca gggggtctcc atcgactaca tccacggcga
     2281 aggggccctg gaggccctgg cccgcacccc ggggagcgcg ggctttttcc tccctcccgt
     2341 ggcggaagag accttcttct cccgcatcgt cacggtggga gtctaccccc gcaagacctt
     2401 ctccatcggc gaagccgccg aaaagcgcta ctacctggag gcccgcagga tcaagccgta
     2461 gggaaaaagc gctccgaaga cgcaccgggg gccgaagatg tcggcccccg gtcttttttc
     2521 gtcaccaatc cccgtctcgc accaagtcgt cgtcgtccac cggcacccga tcgtaggtcc
     2581 aacccttggg tttcggcgcc ggggctccgc ttccccaccc ctcatcccct tcgtcgaaga
     2641 tgtcgctcca ggggaagtgg tactgctggt aggtctcaaa gcggatgggc gtggggggca
     2701 acggctcccc cgtgatggcc cgccagaggc ggccgttcac aatgtgcacg tagatgcgcc
     2761 ccggacgccg ctgatcccag gtatcgtacc cgtaagagtc ctcggcgatc cgctgggaga
     2821 tctttccccc ctctcccaac cccatgcccg gagagccctg ggccgatccc aacgaggagc
     2881 ccttcccctg ttcgccaaaa cgatccctcc aaaactcctt ggcggggtag accatgatct
     2941 gtatgccccc gtaacgttcc tgcccggtga gctgcccctc caccgtgcca ccagatccca
     3001 ggggcatggc cacaaactgg cgcacggccc cccgaccggt gttaaagccg tccaaccagg
     3061 gctgatcggg caccaccaga tagtcctgac ggggagaaag gcggtcggac caggaatcac
     3121 ccgtcagggc gttgatcatc cccgccgcca ccttaaccgc cgcaggcccc tgggagctgc
     3181 gaaagtcgat ccatagggcc tcccgctggt acatggggat gatcactccc ccctggcgcc
     3241 gccatgaggc gggcactcgg gaggcgtagt catccacccg ccggatggga aatctcccga
     3301 ggcccggggg aaggtcccat ctccggccgt catccggcac ccgcagggtt ctttggaaag
     3361 agatcaccac gctatcgccg atgtagatcg cgtccccccg caccgtcacc ggcaacctcg
     3421 acgccccgaa ggccgccccc gatccccagc acaccgtcag aacgacgaaa agccaccact
     3481 tacttcgcct catgtcggcc acctcctttt ttggtcctta cccaaaggtc ggttctctcc
     3541 ttctgtggac gaacgccctc cgaaagggag catagcatct cttcccagga gaaacccatc
     3601 ggtctttttc ccacagaggc cctcgggaaa gatgacagtg cttttaccga tgcctccctt
     3661 cggggccttt tgctcacttt tggcaagggg actccccgaa aatcccatct atcgtgtaga
     3721 ataatgggcg tggatccgtc gatgaagcgc gccgccgcct cggtcgccac ctttcggaag
     3781 atcgggcggt tggcctgagg agctttcact gaggacaact ccataccgta ccttcgaccc
     3841 gccgcacctc tcgcaggggc gccgggcaat gggtggggga agcgattccc ccgcctgccg
     3901 attttgcaaa gaaggggacc tctgaccggg gtctcctttt gtgttatcgc cttttggtaa
     3961 cgctggcgcc tcttccgcca gggagaaggg agtcagcaac gcactctacg agggagggat
     4021 ttttgtgcgg tggctcaaga cctatgcgga ccggtgcatt gggtgcggca agtgtgcgga
     4081 agtttgttcc accacctatt tcaaggaaaa taatggggcg tgttcccgca tccaggtgac
     4141 cgatccggga gcggtgcgca cgctgagcgt ctgcaatcag tgcggcacct gcatcgccgt
     4201 gtgccccacc caggcgatct cccgggataa aaacggcgtc gtccagatcg acaagaaaaa
     4261 atgcacatca tgcctcatgt gcgtcggttt ttgccccacg gggaacatga tggcttacgc
     4321 cccaaaacag tcggagccct ttaagtgcat cgcctgcgga ctctgcgccc ggagctgtcc
     4381 cgcccaggcc ctggaactaa aaaacgacta gtgtcgaaca aaggaggaac ggggaaacca
     4441 tgacgcacga aaaaatcgct cccatgaagg tgttgcaaga gtggagctac gtccggtcgc
     4501 ccatcgaccg gggctacacc cgcaaagccc tctatgtcaa tgtgggcaag cccgatatgc
     4561 agattcggga gattccccag gagaccatag acgccttcac cggaggacgg ggtttcgggc
     4621 tggccctgct gtggaacgcc gtcaccgagg acaccaagtg gaacgacccg gaaaacgagc
     4681 tcgtcatctc cggcggcccc ctgtgcggta tcacccagta ccccggggcg gggaaatgct
     4741 acaccgtttt cctctctccc ctttcggagc agacctacga cagcaattcc ggcgggtact
     4801 gggggcccct gaccaagttc tcggggttcg acgccgtgga gatcgccggc aaggcggacc
     4861 gggacgtggt gctcttcgtc aacggcgatg agggaaagat ccagatcttg gaatccaccc
     4921 ttcccgaatc caacgcctac accgtcaccg aggcgctgca cgactacctc gccgcagatg
     4981 agggagacaa ggtgaatatc tccgtggttt ctgcgggcaa ggcggcggaa actaccctct
     5041 ggggatgtct gaacatgagc ttctacgacc cccggcgcaa gaccgcccgg ctgaagcagg
     5101 cagggcgagg gggcgggggc acggtgttgc gggacaaaaa gatcgtggcc ctggtggtga
     5161 agtactcctc cgtcaagggc accgaaaata accccgtgga tctctccacg ctccaaaagg
     5221 tgggggtgaa gctgcacaaa gaaatccgcg acctcgacaa cgtccagtgt cggatgcgca
     5281 aggtgggcac gcctcacctc aacgagatca tggacgaata ccacctgctc cccaccaaca
     5341 actacaagtt tggccagaac aaaaaagaca tcggcaagat cgattcctac acctacgaga
     5401 agatcttcac ccagggactc cccgacggtt gctggtacgg ctgttccctg gcctgcgcca
     5461 aggcggtgga ccactacgag atccaaacgg gcccctgcaa gggacgatcg gtcacggtgg
     5521 acggcccgga gtacgagacc gtggccagcc tgggctccaa catcggggta tacgatcccc
     5581 tctggaccgt ggaggcaaac ttctacgcgg accactacgg cgtggacacc atttccctgg
     5641 gcacgggact cgccttcgtg tgcgagtgct acgaactggg ctacctggac aaggccaaga
     5701 ccaacggatt ggatctgtcc ttcggctcca aggacgacct catggagctg atccaccgca
     5761 tggcggaggg cacggacgac ttcgcccggg ccgtgggact cggcatcaag aaaatgaaaa
     5821 aagtttttgc cgaacgctac ggagcggatg gggccgtgct ggaaaagatc ggcatggaag
     5881 cccaggggat agagacgtcg gagtacgtcc ccaaggaatc cgtggcccag tggggcggat
     5941 actatctgac gctcaagggc ccccagcacg acgaggcctg gctcatcttc atggacatgg
     6001 tgaataagca gctccccacc ttcgaggaca aggcggaggc cctgcactac ttcccgaact
     6061 tccgcacgtg gttctctctg gtggggctct gtaagctccc ctggaacgac atcgaacccg
     6121 ccgacaacca cgtgcgctac cgtccccagg acgccgcccg ggtccccgag cacgtccaaa
     6181 actacgtgga catcttcaac gccgtcacgg gcaaacagat cgtgaaggaa gacctgatca
     6241 ccatggccga gcgggtgtac aacttccagc gcctcttcca ggtgcgcatg aagcacggga
     6301 acgcggaaca caccatcccc gcccgggcca tgggccccgt cttccccgac gaatgggacg
     6361 cccggaagga gtactacgac gacaagctga gagaagcggg catcgccccg gaagggctct
     6421 ccgtcgagga gaagatcgaa gggctccggg gtcaccgcct ggagcagtgg gccgacctga
     6481 aggatgcggt gtacaagcgt cgggggtgga acaaaaaggg catccccacg gaagagacgc
     6541 tgaaaaaact cggcatcgcc ttccccgaag cggtggcggt ggcgaagaag tattgccgcc
     6601 ccgaggacga gttttagaaa gccatgatcg ttgtaaacgg cgaagagctc ccctggcacc
     6661 cgggcatgac ggtacagcag gtcatcgacg ccaaacgcta cacctttcgc atgctggccg
     6721 tatgggtaaa tgacgtcccc gtcctccggg agcgcttcgc cacccacacc gtggccgatc
     6781 gagaccggat ccaggtactc cacatgatca gcggaggcta gccccggggg aggggctggg
     6841 ggcagacaat aaagaagatc ccccgggcgg taccgcccgg gggatcttct ttattgtccc
     6901 ctggagatat ccccggggcc cgagggccta ctgccctcct cccagctgag tctcctggtt
     6961 atcgagttcc tgaagttgct gctgaaggag gcggagttgc tcctgcacgg cgttgcgctg
     7021 gttctggatg ctcgcccgca gggctccgtc atgacggctg cccgcgtcca tcaccaccac
     7081 cacctgctgc tgaagctccg ccacgtcctt attcaaggcc tcgagcccct gctcgtagga
     7141 tttgatggtg tcgcgcaccc cctcgacccg gtgtcccatc tgggtccaca tgacgttaaa
     7201 aacaagcacc atgaggacaa aaaagccaag ctgcacgagc caccacttct tggcggcacc
     7261 tccctcggcg acgcctccct gggccccgga ccgggcctga gacgacattt caagggattg
     7321 ctgggaattg acggcacacg actgattact catcaaagca tcctccttcg agagtctagc
     7381 ggatcccccg gggatccttc cacccctttt ataccacacc ggcgcacaaa acgcagcgtc
     7441 ttttcggtaa aagccacgaa aaaagcggca ccgggatcgg tgccgcccca aaccacccta
     7501 ggcccggaga tccagctggc ccccaatacc cacgctggcg aagagcttgc tgatgagatc
     7561 ctgggccaga ttatcctggc tcttcatgat cttcgccgcc acggccgtct ggatctgcac
     7621 gtcggtccgc gccttatccg ccgccaacac gccatcgatg gcacccatga tccgtcacct
     7681 ccccgggaag ctcgtcccac tggcgaggca aagcctctcc gcccttatgg tactacctcc
     7741 tggagaccct gtcaacgatc cttccagcga cagcgataaa ccactgcggg gggcacgttg
     7801 aggggaacga agcggaggga gatgtcgtca aaaacctggg ccaactggga acgcatctgg
     7861 agcgaatact ggtaagccac aaaaactccc gagggcgcca ggcacgcctg gatgttttcc
     7921 agcaaggtct ggcgcagttc ctgagggaag accgcgaagg gaaggctgga gatgatgcag
     7981 tcgaaggcgg ggatccgttc ccgatccatg atctctttca attgggaggc gtcccggtgg
     8041 atgtcgcaat cccggaactc gtctttcaag ttttctcgca tggcgtcatc cagctcgaag
     8101 accagtaccc gggctcgggg ggcccgacgg cggacgatat cgcgggtcaa gatccccgtc
     8161 cccgccccca gctcggccac gaaaaccgct cgcttccaat ccacgggaga aagcatctgg
     8221 cgcaccaaaa aacgggagct gggcaccacg ctccccacgg tgcccggaga ctggagaaat
     8281 ttacgaaaaa aaagggcgtc ttctttgagg tgtttagccc acatggccca cggctcctcc
     8341 tttcaccagg gggaatggaa cgtcaaaaaa ctctcgcatt gtagaacccc cggccagaag
     8401 gtcaagggca aaatacgcat tcccaccggg agattgaggt tacccccgag gtaacgcggg
     8461 gcgccccgtc gttttctgaa gaccctctgg ggggccttcg ccgggcaaag atccctatcc
     8521 cccgggggac gcaaaaaacc tccccgatgg accgcgtcct ccgctgctat aatctcagaa
     8581 ggaggctaag gtcatcgtcc cggaggcgct gtgacaatat cgttacactt tttccacgcc
     8641 gccctgacaa caaaaaatcc tccggagtta tagtgcaaag cacggaggtg agaaccatgg
     8701 aaagaagagg gtcttttagg aaggcgttgg gaattttggg agccgccctg gttctggggt
     8761 ggtcgggggc gacggcggcg gtggagtttg acgtggcggc cgacactccc ctggtggtgc
     8821 ggtccggaga agggaaggtg atcgttcgag ccctggtgcg ccccgaagcg gggcggcgcc
     8881 aagagagggc acccttggcg gtggccctgg tcctggacaa gtcgggatcc atggaggccg
     8941 atggcaagct ggagcacgcc aagattggcg ccctagaggc cctacaggtc ctggatcccc
     9001 gggacatggt aacggtggta acctacgatt cttcggtgcg agtggcggta cgcccccggg
     9061 aagtggggca aggagcccgg gattcccggc tgacccgggc catccgccgc cttagccccc
     9121 agggaaacac ggcgctctat gagggcgtgc tcgtggcgat ggaagagctt cttcccttcg
     9181 cggaggaggg gtacattccc cgcctcgtgc tcctctccga tggccaggcc aacgtggggc
     9241 ccacctctcg gggagagatc caggccctgg ggcgccagtg cgcccaggag gggatcaccc
     9301 tcaccaccat cggcctgggg ttggattaca acgaagatct catgacagcc ttggcatcgt
     9361 ccagcggagg caacgcctac ttcgtccacg aaccaagcac gctgccggag atcttcggcc
     9421 gagacatgaa agacgccgtg tccatcgtgg cccgaagggt gcgcatcaag ctgcgctgcc
     9481 cggaaggggt gcgtccggta cggtctttag ggcgcaccgc cacggcggaa gaggaaaact
     9541 ccctcgaggt gaccatagaa aacctgtacg ggtcggggga aaagtacgcc cttttcgagc
     9601 tggaggtctc tccggatgag gaggcccttt cccgggacat cgccacggtg gaactctcct
     9661 acgtggatcc ggccacggga aaggagcttc gggaggagcg ccgcctcgcc gtgtctttca
     9721 cggacagcga gggagaggct tcccgggccc gccgggagga gatcgtttcc caaacggccc
     9781 tggctctgaa cgcggaggcc cgggaaaagg tgctggatct ggtggatgaa gggaacgccc
     9841 aggaggcgcg aaaactcctg catcaacgcc agcaggccct cgccgccatg ccagcggccc
     9901 cgggatcggg ggtagcgccg tcgtggttgt cggacgaaat cggcgctctg gggattttgg
     9961 aggaatccat cgcctcttcg gggacccttt cttcttacgc acgcaaacag gccctcaacg
    10021 acgcctacgt gcagaaaaac cagcaggtcc ccgcgaccac gagccccgac ctgagtcccg
    10081 acataagccc cgacataagc cccgatcaac ggtgacggca cgggtccgcg atggtacctt
    10141 tccccaggag aaggggatag gatcggcggg ggagatctcg cgacgggggc ggaggttttt
    10201 cccgccccgg tccgccccct ttgtccccta tccccccgat ccgaggtttt acggggaccc
    10261 ttcagggcga cgcccccgtc ccgaaacgat atgatggggc ccgatgggag gaccactcat
    10321 gaaacttttc ttgaaacacc cccaaaccct tcgccgcccc ctggccctcc tcttccttct
    10381 gggggtcctc ctgcccagcg tggccctaag cgtcttggcg ctccgggccg ccagccgaga
    10441 ggctctcttt gtggaacggc ggctggaggt ggccctcctc gccgaggtgg gggacgccgc
    10501 tcgccaggtg gagagagccc tggaagatat tagagaagag ctatcccaag agctaaagga
    10561 tcttcccctc acgccccggg acctggcccg gtggggatcc cgccaagagc tggcggcggt
    10621 gcccttcctc ctatcccagg gggtcctgtc cctcccgggg ggaagtgcgg cggagaaaaa
    10681 ggcctttctt caggacttcg gccctttcct ccggggagag ggggctctgc cggtctacga
    10741 ccgcgccgcc gacgtctatc ggcgacgcgt gccgctcccc gagtcccctt gtcccccgga
    10801 ggcgatctcc cgggtcgtcg ggagaggtgc cgccccgcct ctccccgaca tcctctccgc
    10861 catccctccg gaaggggacg ccggcgcccc tccagcgccc cgggagggtg gggtccagag
    10921 ctgggtcccc ctggcggcgc ttcgggaagg gcccttcgta tctcccgaag actcctcgga
    10981 tcgggatgaa ccatctccgg gctccatcgc ggggattcga agcctggtac gcaacgtcat
    11041 cccccaggcc cggcccgagg agtgggcctc ggcaggggtc ccatcgacct tcgtggcccg
    11101 ccagagcacc ctggccaccc tgcgacgcca gggttcttcg gggtttattc cccgcctttc
    11161 ggatggggga ttggtccttt tattttggtc ctccggggaa aatggcacgg tggcgggggc
    11221 tctgatcagg gctgagcccc tgcgccggcg tcttctaggg gtcctgccgg agccccgcac
    11281 cgaggatcgg atcctcgcgg tgctcgacga ccaagggaat cccctggaaa acgagggcgg
    11341 agagccgtcc ctggactggc ggcggccctt cgtggcccga gagatctccc cctcccttcc
    11401 ctactgggag gtgggggcct atctcaccga cccggagatg gtggcatccc aggcccaaat
    11461 ggttcggggg ggcgtgtggc ttttggtggc caccctcctg gtggccatca ccgccggagg
    11521 cacgctgatc ctccggtccc tgtcggcgga gatgaggttg gcggggcaaa agaccacctt
    11581 cgtggccaac gtatcccacg agctaaaaac gcccctcacc tccatccggc tctttgccga
    11641 gctcctgctc caaaagcgcc agcccaacga ggggcgccga gaagagtacc tccagatcat
    11701 ggtggcggag gcggagcgcc tgtcccggct ggtggacaac gtgctgacct tctctcgtca
    11761 gggatcggga caaaactccc tacgccacga tcccctggac ctggcggcgc tggcggcggc
    11821 cgtgggggac caactccgcc cccatctgga gcgcgagggc tttgccctcg tcgtggacgc
    11881 cccggagccc ctgccggtga ggggggacga ggagggactt cgtcaagtgc tggtcaacct
    11941 cctgtccaac gcggaaaagt actccgggga ccgacgggag gtggagctag gggcctttcg
    12001 tcgggaggat cgaggggtgg tggaggtgcg cgatcggggg ctgggggtga taccggccca
    12061 cgcggaacgc atctttcagg aatttttccg atccgatgac tccctcacgg ccccccgacg
    12121 cggcacggga ctcggcctcc ccatagcccg acacgttgcc cggggacatg gaggggacgt
    12181 gacctacgcc ccacgccggg gagggggcag cgtcttttcc ctggagatcc ccctggacct
    12241 tccgaaagaa gaggtaaaaa atccatgaac gagcgaattt tggtggtgga agacgacggg
    12301 gccatcctca ccggcctgga agatctcctg tccagcgagg gtttttcggt gatcaccgcc
    12361 cgggacggcg accgggcggt ggagctctgg caccgggatc gccccgatct catcctcttg
    12421 gacctcatga tcccccgtcg cagcggctac gacgtctgcc gagccgttcg ccgggaagat
    12481 cgggacactc ccatcgtcat gctcaccgcc aaggggcagg aggtggacaa agtgttgggg
    12541 ttggagctgg gggccgatga ctacgtggtc aagcccttcg gcgtggcgga gcttttggcc
    12601 cggattcggg cggccctccg ccggggaaag cgccccctca ctcccgaaag gccctcggga
    12661 cggcacgtct tcggcgccgt ggaaatcgat ctggagggga tgaggggatc tctccagggg
    12721 gagcccttcg atctcacccc cctggaggga gccctgctgg cctacttctt ggcccacgag
    12781 ggaaaggttc tggaccgcca taccctgctc gaaagcgtgt gggggatctc caccggcgtc
    12841 accacccgca cggtggacca gcacgtggtg cggctacgcc agaagatcga ggaaaacccc
    12901 ggaacccctc gccacctcca gacggtgcac ggcatggggt atcgtttcgt gcgcaacccc
    12961 ctttaggccg tcccgggccc ctctttcact ccctccgaag agaaacgcct aagccagatc
    13021 cgggcccctt cttccgaggt cacttcccct cggtccacgg cctcctccag ccgggccttc
    13081 agggccccca ctccggggcc cggggaaagc cccgtgaggg cccggatggc gtcccccgac
    13141 actctccccc ccccccaccg atcccatcgg ccacggaggg ccagcgcctc ccggcggtgg
    13201 gcctcccacc agagggcaaa gctccccccc tcctggccat gggcccaaaa aagcgtcaaa
    13261 agaccctcga gccacgccgt cccgtggcgc cgggcgaggc gacccagccc ttccggggat
    13321 ggggggtcgg cccccaggga gtggtggcgt accaaggcct cgacggagcg ccccagcccc
    13381 cggggccaac gccaaagcgc catccgccga gcggccctcc gggcccccag gattccggga
    13441 gccatggaaa gcgcctccgg gtcaccgccc cgaagttcca ctccccggcc cacgtcgtgc
    13501 caaagggccc cggccctgtc ccggagctcc ggggataggg acgccacgcc ccccaggacc
    13561 cggagagtgt gggagaggag atctccatct tcccccggtt ctcgcgcgtc tcggagggcc
    13621 ccaagctccg gcaacaccgc gccaagcatc cctccccgtt ccaagaggtt caggaatagg
    13681 gcggggtcgc cccgaaggcc ccgggccatc tcccgtccca cccgctctcc gggaatctcc
    13741 cccagggccc cggcaaaggg ggcacaggga gccagggccc cgggatccag gatccaccct
    13801 ggtagagtgg cggcaaagcg cgccagacgg agggcgcgca gggggtcctg gcggaggcga
    13861 tcccggggat cgccgttcca tcggaggcgc cttcggaaga ggtcttccat tccccccgac
    13921 cggtccacca gctggccatc cactcccagg gccagggcgt ttacggtgaa gtcccgcctg
    13981 gccagatctt cctccaagga gtgggggtga caggaggcca cctcggccac cccgccctcc
    14041 cggggcacca cccacacccc gagcggagct ttccccacct ccggagcccc ggcaaagaga
    14101 cggcgcagca cctccggagg ggccagggtg acgatgtcca cgtcttcccc cgccctcccc
    14161 ctcaagagat cccgcacgac gccccccacg agaaaggcgg gaaatccctg ggcttctatg
    14221 cgcctcagga catcgaagcc cggcagatgc cccgaaaaat cggatttcat ggcccccatg
    14281 gtcccagaga gcccccgcgc cgaggctcgg ggaaaggtcc tccggacgac ggaatgctac
    14341 aggacccgac ggtaaatttc ttccgcatcc tccagctcca acctccgggg gttattggcc
    14401 atgatccggg tgactttcag cacccccgcc gccagacggg ggatgtcggc ctttttcacg
    14461 ccgaaatccc tcaggcgggt gggaaggcgc aggtcttcca ccagcctgcg cagggattcc
    14521 accagcccct gggccgcatc ccgcaggggg agcccttcca ccgcctcccc cgccaggtcc
    14581 gcaatccggg caaacttgag gaggttcccc aggagattga actgaaagac ctccaccacc
    14641 atgagggcgt tggccatgcc gtgggggatg ggaaactccg ctccgatggg gtaggcgaag
    14701 gcatgggtgg cggtagttcc cgcgatggca aaggccttcc ccgccagcat gcttccttcc
    14761 atcatggcgc tccgggccgc caggttacgt ccgtcggcgt aggccgcccg gatgtggggg
    14821 aagaggaggg ccagggcccg ttcggcgtac atatccgaca gggatgtggc attcaccgac
    14881 gtgtaggcct ccatggcgtg caccagagca tccatgcccg tgtaggccgt caccaggggg
    14941 ggcaccgaca gggtgagctc ggggtcaagg atggcgacat cgggcaccat gaaggggctc
    15001 actatgccca tcttgagctt ttccttgtgg tccgagaaga tcgccacggc ggtcacctcg
    15061 gtgcccgtgc ccgccgtggt ggggatgagg agcttgggaa gcccccggcg atacagcagg
    15121 tccacgccaa aaaagctccg caaggggccc tcgttggtca aaagcaccga caccgccttc
    15181 gccatgtcca ggggagaacc tccccccagc cctaccacgg cgtcggcctc gaactttcgg
    15241 gccgcctcca gggccctctc cagggtgtcg caggagggat cgggctcgat gccgtcaaaa
    15301 agggcgctct ccatgccccg gtcccggagg gccttctcca gggccccggc ctttccctct
    15361 cgtaccgcat ggcccccggt gagcacgaaa acccgagacg cccccaggcg cgccatttcc
    15421 tcccccgcct gggcaaaggt gccacacccc atcaccaacc ggggggtggt gacataggtc
    15481 tttttctcca atcccgtcac gccctttctc gatttcatgc cgcctcctca ccgggaggcg
    15541 gccaggttcg ggcgaatcat atcacacgga agaaggccgt cgaagagttc ttcggaggag
    15601 gggctcgacc tggagggccg ggccccatct ccaccgcctt cggaaagggg aaatcgagcg
    15661 gagtttgggc taaaaggggt gaggcgaggt ttcggtttca aaaagttgac cttttttggt
    15721 atcggcaaag gagttcttga cgtataatgc cccgtagcgg cgccccgtcc ttccccggcg
    15781 cccttggagg aatgtttccc gtggacaccc ccccgatggt tcccataggc ctccgggcca
    15841 agcttcacct cgtcgccttc ggaatcggcg aggcgctggt ccgcgtgacc atggcccttc
    15901 tgctgatcgg agccaccgcc gccggcattt ccgcagagca atccacgccc caccccgcat
    15961 cgtatttcga gcccgccccc tgcccctgcg acgtgccccc ggaactccag tcccgggtgc
    16021 actgcggcta cctttcggtg ccggaggacc ggcagaaaac ctcgggaaag gtcctgcggc
    16081 tggcggtggc ggtgattcac agctcccacc cccgtccggc ccccgatccc gtggtctacc
    16141 tggaaggggg ccctgggggg ggcaccctgg cccatctcgc ggactgggtc gacgccccat
    16201 tgctccgaga ccgggatctg atactcttcg accagcgcgg ttcggggttc tccgagcccg
    16261 acctctggtg ctccgaattg gaagaggccg agtactccat gctggataaa gtcgtctccc
    16321 cggaccggcg ccaagaggcc ttttcacggg cggcccaagc ctgcgcctct cggctggaaa
    16381 gggcgggggt agacctctcg tgctacaata ccgccgccat cgccgccgac gtggaggacc
    16441 tgcgccgagc cctggggtac gcgcgctgga acctctacgg cgtctcctat ggcacccggg
    16501 tgaccctggt gagcctccgg gactttcccc aggggatccg ctccgtcgtg atggattcct
    16561 gcgtccccct ggaagtccag gagtacgaag aagcccctag aaatgccctc cgggccttcg
    16621 atcgcctctt ccaggcttgc cgagaggatc cctcatgcca ccgggtcttc cccgacctgg
    16681 aagggacctt cgcccacctc gtggaacggt tcaacgccga ccccctaccc ctggaagtca
    16741 ccgtcgtcgg agcggagggg gaagaagagg tcatctccat ggctttttcc ggaaacgacc
    16801 tcctggggac cctctttcag ctcctgtacg accaccaggc catcccctat attcccctcc
    16861 tcatcgaacg cctccgccga agggagtaca cccccctggg agccatcgcc agcgacctcc
    16921 tggtgggagg cctgggggat gggctatacc tctgcgtgga gtgccgcgac gaggccctgt
    16981 tcaactggtc ctccgacgtg gcagaaaacc taaacctcct gcccccagga aggaggtaca
    17041 acccctaccc ctacgacctg gagctctgcg ccgtgtggcc ctcgggaaac gcccccttac
    17101 gggagagctt tcccgtggcc agcgacctcc ccgccctggt gatggccggg ggcttcgacc
    17161 ccgtcacccc gccggagtgg agccgcatgg tggcccggac ccttcccaat gccttctacg
    17221 tcgaattccc ctcgctgggg cacggcgtca gcaccacctc ctgcggcgga gaagccgtgg
    17281 ccgccttttt tcgcaacccc ctgcgccccc cggcgccggg atgcctgatg cgcgccgccc
    17341 ctctgtcctt cgcgctggag caaaaggggc aagaatcccc ctacggcatc gccgaccccg
    17401 aaagccctcc cccctcccag gggttgctgg aggcgctgtg gaagttcctg cgccagtcca
    17461 tggagatccc ctcggaagag ccctgatctt tcccccgact ccgaggccta gtatagggcg
    17521 tcctcgcaga ggtcgaagag gccccggcgg gaaggccgtc gggaggccat gtggaggagg
    17581 atgcggagcc gggcctcgta cttggccagg cggcggcgcg ccaggggaga ggggaccaaa
    17641 aacagctctt ccatgcgaag gagaaaggcg tcgtagtcca gcttatggct ccgcaggagg
    17701 cgccccaaga ccaccccccc gtgctcggag accaggtcct ccggcaacag gagctcctcg
    17761 gcgaggcggt gggcgttttc ctcctctccg tcgtcgcggg ggggggaaaa ctctcccagc
    17821 acctcggccc ggtccctgcc cggcgacaac agggcgtacc ccatcatctt cgccagcacg
    17881 tagcgttgcc atttcttgtc cgcattgaac aatccctcca ccacgatgtg tccccgatcc
    17941 cccctccatt ccacccttcc cgcgtgcacc gccgcgtctt tcaggcgatt acaccggccg
    18001 tagtcccggg gaagggcccc catctccacg gaaaggccct gttttcgggc cacctccacg
    18061 aggtccacgg ggggaagcac cacccgggcc tcctggagaa agccccgtac caaccccgcc
    18121 accaccccgg cgtccaggcg cacccccggg ggaaccttta gggaaggctc ctccggattt
    18181 ttttcagaaa cccttttccg ggggatcccg ggagtaaggg ggccctctcc gggagagatc
    18241 tcggggaaat cgaaggccga gacggtgacc ggaggacctt cgaaggcccc ctccccgtcg
    18301 tcaaaccccg cccgggccag ctcatcttcg aactcgtcgt cgtagggccc tccgcgaagg
    18361 aggagggtgt aggcccgttg gagcaccatg taccgacagg ggtctcctgt gggggtatcg
    18421 gggtgatact ttttcacccc ggcccggtag gcctcccgta gctcgtcctc tcccgccgca
    18481 ggcctcaacc ccagcacacg cagacacgtc gcttttcgca tggcctcttc gccgatcctt
    18541 tcatcgataa agcccccgtc gcgtccctgg ggcgggggag ggaagccaaa aaatcccttt
    18601 aggagtatga tggtcctatt ctaccggaaa ccggccgatt ttcggtcagc cggacgcctc
    18661 cggcggcgcc cccccggagt tgtcttttga gaggcggttt ccctcaggct atcccctatc
    18721 ggtggtcccc ggggctttcc gccctttcct tcccccccga cgggacggcg ccccccctga
    18781 aaccatcgac gaattttttt aaagatctcg aaggaggccc gagccgtgcg catccaagaa
    18841 ggctccatcc tactgctcaa actctacgac gtggcggacg aaatcaatct ggaggccatc
    18901 cccccggaca ttttcgccgg gaagctcccc gtgaagcagc ggctcgtgcg cgtggacgcc
    18961 aattccatag aactggtccg cccccccgtg gcggtagatc tggggccccg cgcctgggag
    19021 accgccggcg ggcggctggt gctggacatc gcggctcact tttttgatat gggcgtcatg
    19081 ggagtggcgg cccgcatccc cgtccgggac gtgaccctgg aggagttctc tcgcctggcg
    19141 ctggcgttac agcaggatcc cgaggtggac cgccagttgg cctgggaagt gcgccacatc
    19201 ctggaaggag tcgccccggc cctagagggc ccccactcga ccctcgaatt cgacgaggag
    19261 ttcgcctttt acataatcca acgcgccgat gccccccttt ccatgaaaca tctggaatcc
    19321 catcccctcc tggtgagcat gctccagggg gcctcgcgcc ccgtgagccc ccagcagatg
    19381 gagctggcgc tgaagaactc cttctccttc cacgaagacg aagacctggt ggtgctcaac
    19441 tacaacaacg ccctcgtggt ggaccgggaa gattgcggcg acgtgctcct cctggtggaa
    19501 tacgccgtgg tccaggtact agagatgcgc tactacaacg accttctgac gaaaaggctt
    19561 caggagctcc acgatgacgc cgatcggaaa ctatcactga tggaagcgct cttttcccgc
    19621 caatggagcg ccctatcccg gcgagggatg aagctcctcc tggaaactcg cctcatgacg
    19681 gaccatctca cctcggccgt ccaggtgacg gaggacgtgt actacgccca gatctactcc
    19741 cgggccctgc ggatcttcga cacccagcaa tggctcgcca aggtggacga gaagctccgt
    19801 tccatgaagg aagcctgcga cctcatggaa cacgagatcg aattccggcg ggccaccatc
    19861 ctggaatgga tcgtcatcgc cctcatcgcc ttcgagggca tccccatcat ccgggagttg
    19921 gcccgatggc tcctccggtg acggaaggcc ccttgcaaaa aaaatcgggg ctggcgatct
    19981 ccctcaggag aggcccagcc cctaataagt tcgaggataa ataaaaacca ttaaaaatcc
    20041 cctatcctgc gcccccctca tggccgagaa gcgctccctt tctggggccg cccgccccct
    20101 cctccggacc gagctcggtc gctccccgaa gctctcaccg tggctccagg cgagaaaacc
    20161 atctccccgg ctagggcttc ttcggaaggt actccacggg gtttttggcc tttccgttga
    20221 cgtacaccac aaagtgcaga tggttacacg tggctcggcc cgtgcgaccc accagggcca
    20281 ggcgctgccc ctggaccacg cgctgtccct ttttgactaa aatcttgcaa ccgtgggcgt
    20341 agagagtccg gaggccgttg ccgtgatcca ccaccactcg ctggccgtat ccccgggctg
    20401 gtcccgcctc caccaccacg cctcccttgg ccgccaatat gggcgtgccc ttgggcatgg
    20461 ggatatcgat gccgttgtgg aactgccccc ggcgcacccg gccgcttttg gtctttcccc
    20521 cgccgcgcat gccgaaaccg ctggatactc gccccaccac gggccagatg aggcccgccc
    20581 gggacgaccg gcgccccccc tccaccacca ccgcttcttc ttcggtggag ggtttggacg
    20641 gcagcttcgg gccgaagagc ttgcgctgct ccggggtcat ggccgccagc atggcctcca
    20701 ggtcggaggt ggaagcgtcg gttgttttac cctgggaagg ggtaacggag gaagcctgag
    20761 ggggcttcgg agccgaaggc ctctgggcca ccttggcctc ctgggaccgg cgggcctggg
    20821 ccttgcgggc tcgaacctcc atgagggtgg ccggtagctc cgaggaagat cggggaatga
    20881 ggagcaccat acctacggaa aggggggcct ggggggaaag gtcgttggcc cataggagat
    20941 actgagactt aacatgatgt ttggtggcaa cggcctccgc gtcttccccg gcgcctacca
    21001 cgtattccgt ccacggcagg ggcttttctt tagccggaga cggcttctca gcccctgagg
    21061 ccgtactccc gagaaccact cccaaaaaga gaaaacagag gaacaccgcc gcaaagtgcg
    21121 ttcgtcgagt ccgattcatc gcctcttatt ccccctcgct cttaagacct agatcagggc
    21181 atgggtggca ttatagccca aaaagtcccc tctgtctccg tcatatgacg ggtatgctgt
    21241 ataatctgcg cacagcatac aatatttttg cgctgcgtca tggcagcgtc gccgggtctc
    21301 ccagggctct ttccaccgtt ttaagccatt ggtgcatctg caccatgcga aatatattca
    21361 acgtggtaaa gcgcccgtct tctaagccgc cgcaggaatc gtccctcagg gctccctcgt
    21421 agacccccgg gccgaaggtg aggaccacgt cctccttggg gggataggtg gcccccttga
    21481 cttgacagat caaagccccc cctccgtcgt cgcggacata gcgggagacg gagtaaatgt
    21541 gccgccccaa ggccccggag ccgtcttcga agcgctctcg acgaccgtac ccctcgccct
    21601 tcaggatctc ttctccgtcc aggatctccc gatgccccac gtctttccgc cggtaaaagt
    21661 gccttatata aagccgaagt ccctccaaaa gccgcgacgc cgaggaagcc aatcgaaggt
    21721 cttcttccgg gtccagcgta tgccccgcca tgaggggcct ccacaggagg gagagggcag
    21781 tatcttcttc ttcgccaccg gaagacccct ggctgagccc cgtcaaaagg gcggcgacgg
    21841 ccgccacgac ggggtaaaac caccgctggg ggtggacttt taccagggcc gccaccatgc
    21901 cgtggcgctt gaggggaccc aaaccccgct cccccggatc caggtcgaag ccgaagcccc
    21961 agggagcccc gtggtccctc ccccgggata ggagcaatct ctcctcctcc ccctggaagg
    22021 gaaagcaggg gcgcagcacc cccggagatc ctagttcggg aaagcatgcc agccagagcc
    22081 ctcggcgccg gtcttccgag aggacccgca ggtccctttc tcgaaggacg ctccgaggct
    22141 cgtgcagcgc tcccaaaaaa aagttgagct tgtgccggaa acgatcccgg acgaggggca
    22201 gggtcaaaag ctgggccacc aacgaccggg acgcccggaa gatggtgtcg gcgtcgtagg
    22261 accgcagctc cttcttgccc gtcttgatct gaaggccctt tccggactcc acccaggaaa
    22321 gcctcacgag ttttttcttt cccgtggcca agttctcgat gacacagtgg ggccccttgc
    22381 cgccgagggt caacgacagg gaaagaaatt cgtccactcg ctcgtaggcc agcacttgtt
    22441 tcggttcaaa agccacgcga cctcacctcg cgcctttcta cgcccctccc accccctagc
    22501 ccttctcact ccatctcttc cttgggagaa aaggtcttca gggaactctg gagggattcc
    22561 aggagcgtgt gcacgtgcaa cgcctccgtg cccttataga aataaaaacc cgccttgaat
    22621 tcatactgaa acttcattcc cgtcttggca aagcctacca tgagggccat ggcctggtcg
    22681 tacacgcgcc gcaccaacgc ctgggccccc cccagggggg tctcggggag gatcaggaac
    22741 acgtcgccgt tgcggagggc aaatcccagg tcgcaggtgc ggatcatacc cctcaccagt
    22801 ttggcgaaat cctcccgcac caaacgctcc tccgtcggac tcaccacgcc ccggagatcc
    22861 aggcgcaacc acacccccgt catccaccgg cggtagcggt aatcccgggc gatctcctcc
    22921 tcacagcggg gctttacgaa ggcctcgtta aaaagacccg tgcggggatc gatcaagggg
    22981 accccttccg agggtcggcc ggaagatctg tcctctgtgg gctcctcgga gcgtccgggg
    23041 gaaaggagga agagcacggc cccctcttcc atcaggggaa cctgctcctg cagccagggc
    23101 ttcccctgaa agagcacctc ttgggcgccg atatccatga agctatccac gaacttcccc
    23161 tccaggggag gggggacctc cgccaggagg ggcaccatgg cgggagacac cccccgtacc
    23221 acccccctgg tgtccgagag gatcaccggt aaggggagct ggcccaccac cttggcgatg
    23281 gtgtcaaggc aaggcgggga ttcctccccc gaggcgctgc cctccgcgca ccgggaggac
    23341 acgacctcaa ggccccccag ggccaatccc gccaagagta gggccacccc cgcccaagcc
    23401 accaccccgg gggcccccgc cgccagggcg gagggcggaa gcaatatgcc ctgggccatg
    23461 agctgcaggg ctcccctccc ccgccgcacc ccccggagcg tcaaaaggac gtaaagggtg
    23521 ctacaaaagc cataggccag gatcacgccc ccccagaggg ggaggggaag ggccgcctcc
    23581 ccgacgggag accccagggc caggacaaat agcaaaatcg gcaccgaaca gaagggaggg
    23641 atgagcatca aatctccgac ttgcgccacg cccttcctcc cccttcgttt caccgcccga
    23701 ccctcgcgaa actcctgggc atcgcgtccg agtcctcgct ccccatgccc ctgcgcctcg
    23761 aggcccgggg aaaataccgc cacctcggcg ggcttcccca tgacgccacg ccaagggcag
    23821 aaaaaggacc agactagagc atcaacgatc ctgcctggtc ttcccgaagg cgctggtaca
    23881 tcgccagggc ctcctgcatg tgcccccgct ccatatcggc gatgtctgag aagatcttgg
    23941 ccccttcggg gtctgcggcg tggcgcgcca tctgatcgta catctccgcc gcccgcatct
    24001 ccgtgacgta caccgtggag gcgatgcgca ccagggcata gtggtttcga aactcgtccg
    24061 cctgcaccac cagccccgga tcgacgccaa aggaggggtc aagattcact tcctcgccga
    24121 aaagccgggc ataatacccc gaaagggagc gccggtgctg atcttcccac tccgccagcc
    24181 cctcgatctg ctccttcgcc gctcccgagg tctcctgcgc ccactggcga taaaaagccg
    24241 ccgcgtgata ctccgagtgg atggcatacc gcagggcgtg tttcatgtcg aaggctcgtt
    24301 ccgtcatggt ccttccccct taataaaaaa ggtatcgacg cccgtcaccc tcggattata
    24361 ctcaaaatgt tcccgaacaa acaaggggca ccccgggcgc ctaatccagc ggcggtaccc
    24421 cccgctaaaa ggtcgtcgta gggggaaaaa gcgccatcgt ccggggtaga accctttaga
    24481 ctagaaaaag ttcgagggcc acaggcgccc cacccagccg tagacgagct ccgcgtgatc
    24541 cggggagaag gcaaactcgg caaaggcccc catgggaatc acggagcgga gaatcgtcat
    24601 catcccttcc ccttcgtccc ggcgccccac gtgggccagg gccgtttcgc agatgatgaa
    24661 ggccgtggca atgtagtacc accccgtggt ctcgggaaga cgggcatagg tcaccgccgt
    24721 gacggcgcac caggcggccc acccccctcg aaggtaggac tctatcacca tgcccaggaa
    24781 ttctcccaca tcctgggtca ccttccccag acgctcttca ccgggaaggg gcaccagggc
    24841 gtcgcgagct ttggccacca cgggcaggat gaaacggacg tagagcagag cgaaggccag
    24901 ggcaaagagc gccatacgag aggcgtagtt gaaatacaac atgacgttat ctttcctcct
    24961 cggccccttg gccgtaatgg acaaattttt tccgcacccg gaccatctct tcctcgccca
    25021 gcgtcgccgg agtccccacg caccgggcgc gccaccagag ctccgccaca aactcgattt
    25081 cctccgccac ggcgaaggcc cgttccaagc ttcgccccag ggccaacagg ccgtggtggg
    25141 ccaaaagcgt ggcgtcccgg cacgccaggc aggcggcggc ccccctcgcc agctcttcgg
    25201 tgccaaaggt ggcgtaggag gcgcagggca cgtccaggcc cgcaaaggcc accaggtagt
    25261 gcactgcggg gatggtctcc cccaggcagg ccaccgtggt ggcaaaggga gaatgggtgt
    25321 gtaccaccgc cccggcgtcg gggcgacggt ggtagacctc ccggtgcaac gccatctccg
    25381 acgaaggggc ccgagagccc tcccgcaccc tcccctccag gtcgagcacc accatgtcct
    25441 ccggccggag ctcgaagtag tccaatccgc tgggagtcag ggcccagagt ccctcgtccc
    25501 gccggagaac gctcaggttt ccccccgtgc cccgggtgag tcccgccgtc accatccgac
    25561 ggccaaagag caccaactgc tctcgttccc tttcaagaag cacctaaccc acctccccca
    25621 ggtcccctac ggggagcatt atacacagga aaggaacctt gcggagcgga tcggaaaaga
    25681 tgatcgcctc tccggcggac ccgggcgaaa cctgccccaa ttacgggttt gccgcgccgg
    25741 agttatggat ccgaaagacg aggcatcttt gggcttgaca gctccgcccc cggtgttacc
    25801 cttgtcgggc atctttctcg ccacaaaact ggaaagctcg ggaggtttcg aattatgcgc
    25861 atcgtcacca cgcccgctgc ggcgcgagag gccctggcgt ccatgccaaa accggtgggg
    25921 ctggtgccca ccatgggatt tctccacgaa gggcacctgt ccctggtgcg gcgctgccga
    25981 ggagaaaacg ccacggcggt ggtatccatc ttcgtcaacc ccctccagtt cggcccccgg
    26041 gaggacctgt cctcctaccc ccggaatctg gtccgggacc aggaccttct ggagcgtctg
    26101 gaaaccgacc tggtcttcgc ccccgacgcc tcggccatgt acccctccga cgcctccacc
    26161 ttcgtggaag aaacccggct tagcgccgac ctgtgcggcg cggcgcggcc ggggcacttc
    26221 cggggggtct gtaccgtggt gctcaagctc tttcacatcg tccagcccca gcgggcctac
    26281 ttcggcgaaa aggactttca gcagctccag gtcattcgcc gcatggtccg ggacctggac
    26341 gtccccgtgg aagtggtggg gtgccccctg gtacgggagg cggacgggct ggctctgagc
    26401 agtcgcaacg tctacctttc ccccgaggat cgcctccggg cccgggccct ctcccgatct
    26461 ctggcccatg cccgggatct cgtggcccgg ggagagcggg acgcccgcgc cctgcagcgg
    26521 gaggtggagg ccttcctccg acgagatccc gccctggaat tggactacgt ggcggtaaaa
    26581 aaggccgaca ccctggaaga cgcggactcc ttggagggcc gaatcctggt ggccctggcc
    26641 gcccgggtgg gcaagacccg tctcatcgac aacagcgtgc tggaagtctg aggcccccat
    26701 gctcttacac atgctcaaga gcaagctaca ctgcgccgtg gttaccgaag ccgacccgga
    26761 ctacgaagga agcatcgcca tcgcccgaga cctcatggag gcggcgggac tcctccccgg
    26821 agagaaggtc ctggtggcca acgtggaaaa tgcccaccgc ttcgagacct atgtcatccc
    26881 cgcccctgcg ggaagcggca ccatcggtct caacggcgcc gccgccctgc tcggttcccc
    26941 cggagaccgg gtcatcatca tggcctgggg gatcttctcc gaggaggaag cccgggccca
    27001 cgcccccacg gtggtgcgcc taaccaggga aaacggcgtg gaagctcgct ttcccatgcc
    27061 tcggtaggtt tttagaccgg tccttcccga gaagaaccct tcccggggat cttttgtccg
    27121 gcaaaaaggc aaggccagga ccctcgccac gggggcccgg aaaggagagg atcgtcatgg
    27181 agcgtttgac cattccccgg ctctgggaga tgaagcgcca gggggaaccg atcgtgatgc
    27241 tcaccgccta caacgtatgg caaacccggc tggtggaggc cgccggggcc gacatgatcc
    27301 tcgtaggcga ctcgctgggc atggtggagc acggctatga gggcaccgtt cccgtcaccc
    27361 tggacatgat gatcctccac tgcgccgccg tgatgcgcag cagaaagcgc accttcgtga
    27421 cgggggacat gcccttcctc gcctgcgagg tgagcgatcc cgaggcggtg cgctcggcgg
    27481 ggaccttgct aaaagaatcg ggggtggact cggtgaaagt cgagggaggg cgccgccggg
    27541 cccacaccat cgccgccctg acggaagcgg gaatcgcggt catggggcac atcggcctca
    27601 ccccccagag cgccgtgtcc ctggggggct ttcgggtcca gggtaagacc acggagcagg
    27661 cccgccgact cctggacgac gcccgggcgg tggaagatgc gggggccttc gcggtggtgc
    27721 tggagtgcgt ccccgccgaa ctcgccgcca tcgtcaccgc ccagctatcc atccccacca
    27781 tcggcatcgg cgcggggctc ggctgcgacg gccaggtcct ggtcttccac gacgtcctgg
    27841 gactttacgg agacttcaag cccaagttcg cccggcgcta cgccgaaggg gggacgatcc
    27901 tgacccaagg gctggagagc ttcgtccgag aggtaagaca gagggccttc cccgcgccgg
    27961 aacacgcctt cgccttggat cctcgggtgc gagaggagct tgaaaagcca tgatcaccat
    28021 cgtgggatgt ggcgccctgg gaagcctctt cgccgggacc cttatcgacg cgggcttccc
    28081 ggtacagggc ttttcgcgcc ccggagccca tctggaagcc tgtcgccgcc ggggtctcct
    28141 ggtggaatat cccgatggaa gcgagcggac gtaccacttt cccctggagg actcctggga
    28201 acgcctcgag cccagcgacc tcatgctggt gctggtgaaa acccaccaga ccgccgccgt
    28261 ggccccccac ttgccatcgg ccctaaagcc ccggggaatg gtgctcaccc tccaaaatgg
    28321 tctgggcaac gccgaaatct tggcctcgtc cctttccccc ggccagctcg ccctggggac
    28381 atgctcctac gccgcctttc gccccgcccc ggggaacgtg cgctggggcg gaacggggga
    28441 gatctggctg ggcccctggg accccaccac cgatgtctcc cctttgatag cgtcctttca
    28501 gaaggctggc ctctccgccc acttcactcc ccatccccag agggccctgt gggagaagct
    28561 cgtcgtcaac gcctccctga atcccgtcac cgccctggcc caatgcccca acggcgaaat
    28621 actccgccac gatggcctcc tgaacgtcgc ccggaccctg tgcgatgagg ccgtgcaggt
    28681 ggcccgggcc gagggcttcc ccctggagga ggagaccctg tggcaaaggg gcttagccat
    28741 tctggaccga accaaaaaca accgttcctc catgctccag gacgtggagg cccggcgacg
    28801 caccgaggtg gacgccatca gcggcgccgt gctggaccgg gggaagaaac atcgcctccc
    28861 cctgccgatg acttcctgcg tcaccgccct gatccgggcc ctggacgccc agggggatcg
    28921 atcctagagg atctccaatc tttccgggga aaactggaaa aaaagagtcc ccgggggccc
    28981 ttagtccccc ggggacttcc ggcgactccg gcaaaacccc tttacccgcc catctctccg
    29041 gggcgcgtgt cctcaagccc cacgtttcgg ctgtcgggca tgcacaccat gcccccctgg
    29101 gcgtcgacga gacactggga gatctccacg ttggggtcca cctccgaccc agtgatgtct
    29161 ttcaggaaga aggccaggct cttcaagctg ggggattccc ccaagggaaa gatccgggag
    29221 tagatcaccg tgccctcggg atccaccacc accagggccc tctcggaaaa gccctcctct
    29281 cgcaggatgc cataggcttg gctcacggcg ccgtggggcc aaaaatctga caaaagcctc
    29341 aggctcttca gccccaggtg ctcccgccaa gccttcttgg tgggaccggg gtccacgctc
    29401 actccgaaac ataccgcgcc gaacttgtca aagagtcccc gatgacggtc cagggtgcgc
    29461 atctggtcgg cgcacaccgg ggtccacgcc agggggtgaa aggcgagcac cacgtacttc
    29521 cccctgtagt ccctcaaagc cacgtcctgg tcgaactggt cccgcaacac gaactccggg
    29581 gccaccgctc ccgcttccac aatcttacaa gccatgacca tacctccttc gacacggtta
    29641 ccttcccgat ccatcccacc gccgccaggg cttcaacgat agtgggcttc cagaaactcc
    29701 cgcacccccc gggggtcccg gggacgacga aaaaggggac gggcgtcgtc gccgtaggcg
    29761 ctccgggttt cctcaaacgt gggatgagga ttcacgaaaa gcacccccag gggccaccgg
    29821 tcggcctgaa gcgccagggc cagggcggcc tgcggatccc gggggttgtg ttcgggtccc
    29881 acatagtacg tattgtcgcg ataccactta aacgtattca ccttgttgaa agagacgcag
    29941 ggctgcaaga tgtccaccag ggcataaccc ctgtggagga tggcgcgctt catgatttct
    30001 tttgttttat ccacgtcgcc ggaaaaggcc cgcgccacga aggccgcccc cagggaaatg
    30061 gccaccgcca gggggttcat ggggaggttc gatgtccctt cgggggaggt ggggcttcga
    30121 aaccccaggg ggctcgtggg ggacgcctgt cccttggtca gcccgtagac catgttgttg
    30181 tgcacgaagt tggcgatgtc cgggttgcgt cggatggtat ggagaaagtg gtttccccct
    30241 tcgccgtaca tgtccccgtc gccgccgatg gccagcacca ccagctcggg gttggacacc
    30301 tttatggccg tggcgtggga aagggcccgg ccgtggaggc cattgaaaaa atggcacctc
    30361 atgtagtggg gtagcttggc cgcctggccg atgcctgaca ccgccaccac ctggtggggc
    30421 tccagatcca gctccgccaa ggcctttttc aaggcctcca agatgtggaa gtctccgcac
    30481 ccggggcacc aggatatctc tccgggattt tccatgtcga agatcgcggg atctttcacg
    30541 ccatctcccc cccttcgtcc agggccttcc ccaactcctg ggccacccac tccaccgaaa
    30601 aggccaaccc gttccaccgt cgcacggagg cgtcggggcg tcgccccgtg gccaagaaga
    30661 ggtgccgggc gaattgcccc gtggcgtttc cctcgaccgc caccactcgc tccgcccggg
    30721 ccaagaaatc cttcgtggag gggggtaagg ggtagacctg ggggaaagaa agcagggccg
    30781 tatccttcct tcccagtcgc gccagggcct cttcccccac aggacgggtg gagccccaca
    30841 ccaccaccag acatcgatgg tcctcggggc ccacgaggac ggggggcagg gcgtcccggg
    30901 tcagaccttc gagctttttc atcctcttgg cgtgcatcct ttggcgcagg gcgaagtctt
    30961 ccctcacgtg cccctcttcg tcgtgttcgt ggctatccgc ccccacgagg ccttcaccgt
    31021 gtcccggcac cccccgggga gacacccctc cttcggcgta ggcatagcgc cgataccctt
    31081 ccttggtctc tatcagataa tctccggtgg cgtggcgctc cagatccgga atgggaaggt
    31141 cgtgggagag atccaccagg tactggtccg tgagcactaa cgcgggactc tgccacttga
    31201 gcgcctgggc aaaggctctc tgggtgcaga aaaaggcctc ctccaaggtc cccggagcga
    31261 agatggcccg gggaaactcc ccatgccccg catagagggc caaatccaga tccccctgct
    31321 ccgtccgggt ggccatcccc gtggcgggcc ccggtctctg ggccaggtgg atcaccacgg
    31381 gacattcagt gatccccgcc agcgagatcc cctctcccat gagggccaat cctccccccg
    31441 aggtgctcac cagggggcgg gccccggcat aagaggcccc caccgccatg ttgatggcgg
    31501 cgatttcgtc ttcggcctgc tcggtgacca cgccgaaccg gcgcgcctgg gcgacgagaa
    31561 actgaagcac ccccgtggcg ggggacatgg ggtagccgca acagaagtcg cacccccccg
    31621 ccaaggcccc cagggccacg gcctcggtgc cgctcaccaa tatggccccc tccacggcgg
    31681 gatcggcgac gaggtccatt tctaaccgcc ccgaccgccg cagggcctcc ccttcggcgt
    31741 acccccgggc cccggcggcc cagttcttat ccgccatatc ggtgccccgg cccccgaaat
    31801 gtcgtctcag ggcctcttcc agcttctccc gggggatttc cagcagcgcc gccaggactc
    31861 ccaccgccac tacgccagca aagagcgcct gccccacctc ccgggccagg gacaaaaaag
    31921 acacggggac cgtctggccc tcccagcgtc gaaactcgtc ccccagctcc tcgggatcgc
    31981 ccaagatggc cgttcccccg tcgatgcgct ccagaatgtg ggaccgcacc cctcgattca
    32041 ggggaaggag tacgtcgatg cgatccaccg gtgccctcac cggaaaggat gaaatccgca
    32101 gggcagtgga attgcttccc ccccgcaccc gagacatgta ctcccgactg gcaaagacgt
    32161 ggtaccccgc cgccttggcc agcttcacca gaatctgctc caccgtctgc actccttgcc
    32221 ccgcagcgcc gcagagcacc aaggagacgt cgccaagatt tgccctcgga taattggcca
    32281 tattctccct ccccgacgaa agccctttgt ctccttcgat ctcaagggct caccggccct
    32341 ggaaagacct caagacccac tggcagtgta gcatcttccc gccctttatc acagggaggg
    32401 gcccaccttt tgtccccgcc cccgaaacaa agggacggag tcggtaaaat cctctggcgc
    32461 cccggcccgg gaggacgtat aatgctttta ccactctttt cggaaaaagg agcttttcca
    32521 tggataggcg tcgggccctg ggagtcggcc tgctggtctt ctccctcgta agttttgctg
    32581 tggcactgcg ggataccctc cgggacttcg ccaccccatc cccaagcggc ggcgcccccg
    32641 cttcctccgg gcgtcatctg gccatctacc tcctgtacgg caacgccaag tgccccacct
    32701 gcgacgacat catgaggctc acggaggaga ccctccgaga ggacttcgcc gccccccggg
    32761 agcggggaga gatcgtcttt tcctccgcca acgtggaaga ggcgggtaac gagtggtacg
    32821 tgagcacctt cgaaattttt tccacctcgg tggtattggc cctctacgac caggggcgcc
    32881 tggtgacgtg gaaaaacccc caagaggtct gggacctcgc cgccgacccc gccgccttca
    32941 aggcctttct tcggcgggag atccaaagca tgctctcttt ctccccccag ggaccagaaa
    33001 atcccggagc cgccgcctcg gaggaccacc cctcatgagc gccatcgtca tcgccgcctt
    33061 aggcgccaca tggctcggca tcctctcctc cgtgagcccc tgccccctgg ccgtgaacgt
    33121 ggccgccatc tcctacctcc tccggggggg acgcacggga cgggccttcg ccgagagcgc
    33181 ggccctttac atcctgggga ggtccttgat catggccctg gtgggctttc tggtggtgcg
    33241 gggaatcttt gccgtccccg aattggccat gatcctccag aagtccatga acagatttct
    33301 cggccccctc ttcgtggtgg tgggggtctt cgtcctcgac ctagtccccg tgcgctttcc
    33361 ctggaccaaa aaagtaggca aggtgcgctt cggggtggac cgagggggga gtttcctcat
    33421 gggggtggtg ttcggcctgg ccccctgccc cgtcaccgcc gccctctttt tcggggccct
    33481 ggtgcccatg gcggtggagc acgcctcccc cgtcctcctg cccctccttt tcggggcggg
    33541 caccgccctg cccgtggccc tggcggcggg gctggctttc tttggcgttt cggtgctggc
    33601 taaggccctt ctttccttcg ccaccctgga tcgatggctc accctggcca acggcgccat
    33661 cttcataagc ctggggctct cttacatcct cacctttgtg tttcccatcc tttaggccgg
    33721 gaggtatttt tagttggaaa gcacggtcgc aaccatcccc atcctgggta gcctggccaa
    33781 ttccggggcc atattggcgg gaggattcct gggagtgctc ctgcgctccc gttttcccga
    33841 gcgcctctcc accatcgcct tccaggtcat ggggcttttt tccctatccc tgggggtttc
    33901 catggcctcc aagagcgcca actccctggt gctgatcttt agcatcgtcg ccggagccat
    33961 ggcgggagag ctcctgcggc tggaagacca caccgatcgc atggcgacca agctcaagca
    34021 gcgcttcgcc gcccacgacg aaggtttctc ccaggcgttc atgactacat ttttgctctt
    34081 tgccatggga tccatgggaa tcttgggggc catcgaagag ggattaggaa aggggcccca
    34141 cctcctctac atcaaggcca tgctcgacgg cgccgcctcc atgatcctgg cggcgacctt
    34201 aggccccggg gtgctcgtag cggccctgcc tctgggggtc taccagggaa gcatcgccct
    34261 tctggcagga cagctcgctg gcgtcctcac cccccctgtg gtgaacgaaa tcagcgccgt
    34321 tggggggctc atgatcatgg gcatcggcct atccctcatg ggagttctca agatcaagac
    34381 catgaacctc atgcccagta tcgtggtggc cgcaatcttg gggtacgcct tctgcccggt
    34441 ctaacccccc tcgccgccct cggaggaaaa actccggctt cggggcccat ctctaggaac
    34501 gccccgcgaa ctcgaggccc ggggggcgcg aaagaaggtg ttgacattcc gagaagccct
    34561 cggtatacta tgcctcgcgc cggtcaaacg gggcaagaag ggcggatagc tcagcggaag
    34621 agcacctgcc ttacaagcag ggggtcgttg gttcgaaccc aattccgccc accacatgac
    34681 aacagaggtt gaggccacgc ggggttgtag ctcagttggt tagagtgccg gcctgtcacg
    34741 ccggaggtcg cgagttcgag tctcgtcagc cccgccattt tccctcttgt cgtgcgtcga
    34801 tctgggcgag atagctcagt tggtagagca gcggactgaa aatccgcgtg tccccagttc
    34861 aattctgggt ctcgccacca tcttttggaa agaagcggaa gtagctcagt ggtagagcac
    34921 aaccttgcca aggttggggt cgcgggttcg agccccgtct tccgctccat ttcttccgta
    34981 caaggccccg gggcggcata gccaagtggt aaggcagagg actgcaaatc cttcaccccc
    35041 ggttcaaatc cgggtgccgc ctccaaaata tcgtttcacc aagttaccaa agggtttttt
    35101 gcagttcggc aacaacgcgt gtgtgaggaa attggcaatc aacacctttg gttttttatt
    35161 cctcgatagc tcaatggcag agcgggtggc tgttaaccac taggttccag gttcgagtcc
    35221 tggtcgagga gccaaaattc aatccgctga tttcagcggt tcttgtttaa cgcaagacc
//
LOCUS       DAOOER010000016        33811 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_1443_length_33811_cov_8.461162, whole genome shotgun sequence.
ACCESSION   DAOOER010000016 DAOOER010000000
VERSION     DAOOER010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 33811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33811
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1443_length_33811_cov_8.461162"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="PL162_03715"
     CDS             complement(<1..179)
                     /locus_tag="PL162_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HQF91165.1"
                     /translation="MEVKVYSTPTCPWCTKVKEYLKGKGVTFTSVDVSQDRDAARDMV
                     ARTGQMGVPVIDIDGT"
     gene            414..911
                     /gene="nuoE"
                     /locus_tag="PL162_03720"
     CDS             414..911
                     /gene="nuoE"
                     /locus_tag="PL162_03720"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HQF91166.1"
                     /translation="MAVATATREELVRRLDPIVERHRGKKGITIPLLSDVQKVFGYLP
                     EDAVEYVGKALDIPAAELFGVATFYAMFRLEPQGKYVVRLCRGTACHVQGSGRIGEQL
                     QRALEIQEGGTTADGMFTLQYVACLGCCSLAPVMMVGEEVHGRLTPEKAVEVLEGYRA
                     RRVEP"
     gene            908..2677
                     /locus_tag="PL162_03725"
     CDS             908..2677
                     /locus_tag="PL162_03725"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HQF91167.1"
                     /translation="MTKTVVRVGVGSCGIAAGALAVLHRLSEVLGSREDVVLKPVGCV
                     GLCFHEPLVEIEREGQRWLYGEMDAAGAERVVRDFLATGEAPAEHLVYSTVTEAPEND
                     MLGKQVRVVLRNCGIIDPEKIDEYIARDGYKALEKAVHSMAPEQVIAVVTESGLRGRG
                     GAGFPTGLKWKFAREAKGDLKYVVCNADEGDPGAFMDRSVLEGDPHTVIEGMVLCAYA
                     VGASQGVVYCRAEYPLAIKHLNLALEQARERGYLGRNILGTTFSFDIYVKEGAGAFVC
                     GEETALIASIEGKRGMPRPRPPFPAQSGVFGKPTNINNVETFANIPWILVHGAEAFNR
                     YGIGKSRGTKVFALAGKIVKGGLVEVPMGMPLREVIFDVGGGVPDGKTFKAVQMGGPS
                     GGCIPAALLDTPVDYESINATGAIMGSGGMVVMDEATCVVDVAKFFLSFTQRESCGKC
                     PFCRIGTKRMLEILTRISEGQGRPDDVDLLLEMSVQIKDGSLCGLGQTAPNPVLTTAK
                     YFREEYEAHIRDHQCPAKVCSALIHYTIDAEKCIGCTKCARACPVKAISGALKAPHVI
                     DDGACIRCGLCLKSCPVKAISVE"
     gene            2696..6073
                     /locus_tag="PL162_03730"
     CDS             2696..6073
                     /locus_tag="PL162_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQF91168.1"
                     /translation="MKERTIRVLLDGREVFGFPGQRLLDLCAECGVEIPALCYDPHLS
                     IHGGCSICLVEVKGAKALARACATTIGPDMEVTTRSERVRKARKLGLELLLSDHVGDC
                     RPPCTLACPGRGDVQGYVNLAAQGKWEESLEALRRNITLPASIGRVCPAPCEKKCRRN
                     LVDDAPVSIREIKRVVGDVCLHEGTMGHIPEIVENGQSVAVVGAGPAGLSAALFLRRK
                     GYKVDLYDKEPLFGGMMRYGIPDYRLPQDVLQAEVEWILANGVTAHPNTALGRDVSLD
                     ELRESHDAVLLALGCWKSSPMRCAGENLPGVWGGINFLYKVNRREPVAVGRRVAVVGG
                     GNTAMDAARCARRYGAEKVFVVYRRTQAEMPAEPVEIQEAMEEGVEFLFLAAPKAVEG
                     EGRVERMVCEKMALGDPDASGRRSPVPTGETFVLEVDTVVAAIGQAADFSALPKVLHD
                     GRRPLVDENYATPLPGVFATGDFQTGPDIAVAAMGGGHWASESIHAWLTTGVAKRPFE
                     YDVVQKDLTSADFAHVERTHQEHPHHDAPEARLAPDRAFQEFNHGLTPEQCKTDAARC
                     MECGCGDLFECKLRRYAQQYEVNPDRVAGAHVAGVQEVNSSYVRNMDKCILCGRCVRV
                     CDELAGFHAIDFTRRGFEGSVDAAFWRGTEGTECTQCGLCVQLCPVGALMEKRAPRKP
                     HSEIPQIMKTACGRCGLGCQLELNLDADRRRVVRVTTDFDDPTSVSGGNSCARGRFGD
                     EAVAEKRLAEPRFRGEEGRWNTLLDDLGGKLKGVLDASGGSAVAVVAGGSLGNEEYLA
                     LQRLVTEALPGALLAVEGVDAFAPARQVLETAWKTPLGGATYADVRHADAYLLLESDT
                     DRTHPVLTSWMRGAMRKNHAAVVYIGQDAGKLDRGDALVLSPREGTREDLLRGLSVAA
                     LQAAGRTVPDGWAEYTPLKTAALTGVDEKIIEGAAHALAKAKRLVTLVGAPVGSSGGA
                     MEIVTTLLGILGARRVMLLHEGANVVGAALLNVASASVADVVGALRGGDIKALLTVGV
                     DPAALGMNDDDLKGLAISAALSTERLTTSPDYVLPMADWREREGRAFTLAGVQVEQVR
                     AIPAPGLAVELCWTVAALARRLGREIPVRIPLVSAGR"
     gene            6269..6886
                     /locus_tag="PL162_03735"
     CDS             6269..6886
                     /locus_tag="PL162_03735"
                     /inference="COORDINATES: protein motif:HMM:NF038013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HQF91169.1"
                     /translation="MSGSQQHSSVRIVVADPSALGLFGLAMVTLVASSQKLGLTTGLS
                     FVVPWALFLGSLAQLMACVYDFKHGNTFGATAFGAYGLFWAGVAASWLIRMGVFGPEL
                     AAEVDPRELGVAFLGYTIFSIYMTIGAMETHKVLFAIFVLIDVLLGALAYNTFYPQSS
                     GVHALAAWSELAISLLSFYGSAASVLNSHFGRPFVPVGRPFGWFK"
     gene            7071..8465
                     /locus_tag="PL162_03740"
     CDS             7071..8465
                     /locus_tag="PL162_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HQF91170.1"
                     /translation="MVKRMQPEPFRIKMVEPVSVPDAAAREKALEAGHFNMFGLRSED
                     IYIDLLTDSGTGAMSKYQWAALMKGDEAYAGADSFYALKNAVKEVLGFDYVIPTHQGR
                     AAEHVVFSTLVKPGNILPFNMPFDTTLAHAFNAGGITVNCVIDEAFDPESLLPFKGNV
                     DIAKLEATIAQHGRDNIPFIMVTITNNSGGGQPVSLENLRAVSAVAKKHGLPLLLDAA
                     RLAENAYFIKMREPACKDMTVAQILKATMDTADGITCSAKKDPLVNIGGMICCRTEEM
                     YHALLPRVILFEGFVTYGGLAGRDLECLAQGLREMVDEHYLAHRVGQVKYLGDLLEEG
                     GVPFVKPAGGHAIFIDAAAFLPHIPQKYYPADVLAIEVYREGAVRGIGLGALAFATED
                     EKTGEVKMPKLELYRLAINRRTYTNSHIEFIAQTIVDVYKRRESIRYGLKVEYAPPVK
                     GIHHFLAHLRPFNL"
     gene            complement(8571..9569)
                     /locus_tag="PL162_03745"
     CDS             complement(8571..9569)
                     /locus_tag="PL162_03745"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HQF91171.1"
                     /translation="MAHIVVFGAGDWGTALASVAASNGHRVCLWCRRAPQARAIIATG
                     RNPDYLPELALPSNVEATGSLAEAAVFGKRWIAAVPFQALREIWQHLKPLAPHDLRVC
                     NAARGMEALTGLTGSALCHEVIPGAVYSVLGGPAFPEDVAQGHATALVVASSLTEEAL
                     EWQVLLSTPGFRVFISRDVRGVEATMANSVTAIAAGMVRGLGLGDAAMTAIITLGMAE
                     IARRSRNMKGVPGTVLGLAGAGDLWRFCTENSREFRMGVLLGQDKSPEDALAEIKHTV
                     EGYHTARALAAQGCTLAQAVAQVLFNRGKPKKVLEDLIKNAPLGGDFTEEELQRMG"
     gene            9758..10285
                     /locus_tag="PL162_03750"
     CDS             9758..10285
                     /locus_tag="PL162_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQF91172.1"
                     /translation="MLQHEHPAIRTLLGRRSIRRFEGRPVPEDAVALILECACAAPSA
                     ANNRPWHFVVVDDRSKLDALAGVLPYGKALFQAPLAVVVCGDPTKSDMSRAYWEEDCS
                     AAMENILVGAWALGIGSVWLGVHPVPGRSEAVRQILDIPGSVSVLGIAALGYPGEEKG
                     PHAGVDPGVVHQNRW"
     gene            10504..12954
                     /gene="gyrA"
                     /locus_tag="PL162_03755"
     CDS             10504..12954
                     /gene="gyrA"
                     /locus_tag="PL162_03755"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HQF91173.1"
                     /translation="MAEQMGGQLEFSKVVPLPLEEEIKHSYLDYAMSVIVGRALPDAR
                     DGLKPVQRRILFAMLELGLRHNQPTKKSARVVGETMGKYHPHGDQAIYDAVARMAQDF
                     SLRYPLVSGQGNFGSIDGDPPAAMRYTECRLHAMGEEILADLDEETVDWGPNFDDSLK
                     EPLVLPSALPNLLVNGASGIAVGMATNLPPHNVEEVVDALCYLIDHEDAELGELMQFL
                     PGPDFPTGGIIMGRDGIIDAYRTGRGRLLVRGKAEVEERHHGRRAIVISEIPYQVNKT
                     NLIESIAKGVQNKALDGISDLRDESDRQGLRIVLELQRDATPEVTLRQLYSRTPLQTT
                     FGVINLALVKGEPRELPLVDMLSVFLAHRREVVRRRTEFRLRKAEERSHLVEGLLKAL
                     DIIDEVIALIRGSRDGEEALRGLVDRLGFTELQARAILEMRLQRLTGLERTKLEEERA
                     QLLAAIEGFRAILAQAQVRDQVIKDELQEISKRHRDSRRTEIQDGMEDVAQEDLIPEK
                     DIVVVLSRDNFLRRMPLEDYRRQQRGGKGVKGAPPKEEDEISIVAVTTTHSGLYLFTS
                     KGRVFSLRGHVIPQPKTGKGRHVSAFVPLEEGERVMTLRDGRMAGSRFVFFITKRGVA
                     KRMPVTELENITRAGRRVLSLDEGDDIARVRCTTGEEDLLLVTAQGQAQRVNEEEFRP
                     MGRMARGVRGIRLDPGDEVVGCDVVRPGVTSLFLSENGIGKRTEYGEFTVHHRGGGGV
                     RSMRLGDKTGSLVGSWSVHGGDEILVISARGRMVRLGVEDIPLLSRTATGPIVVRLDE
                     GDAVVDVSVFSSAPEDGE"
     gene            12951..13595
                     /locus_tag="PL162_03760"
     CDS             12951..13595
                     /locus_tag="PL162_03760"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HQF91174.1"
                     /translation="MRPVARDGFPLIGGVALACAAGGVYDVHLALGILPLLALVIWFF
                     RDPERTTTAGPGSFVSPADGKVVEVGSAFHPFVGNATRVGIFMSPLDVHVNRAPFAGI
                     VAHLEYVPGARWMAFDPKASERNERFFVGLDTAVGPVIITQVAGFLARRIVCRLIRGD
                     RLERGERYGMIKLGSRVDLYLPPAVECVVAVGQRVRAGETTIGVVRDGPETQGA"
     gene            13570..14310
                     /gene="pssA"
                     /locus_tag="PL162_03765"
     CDS             13570..14310
                     /gene="pssA"
                     /locus_tag="PL162_03765"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HQF91175.1"
                     /translation="MALRRKEREVPFRKIIPNMITCGNVLCGMLSVVLSFHEHYALAG
                     WLILAAVFFDFMDGKVARSLGGSSAFGMELDSLADVVSFGVAPAMVVYGAALRGFLGV
                     TGALAAAFFALCGALRLARFNVTHAPAGSFQGLPIPAGGLFLVSFVMGGVTLPPAIFA
                     AVVVGTGVLMVSNIPYGNLKKLRKGNMNRGKGLFLSLFLLGLGAIFREKAPLMAMGIY
                     VVSGFFRFDWGGWLSKKEGECPAPGEKP"
     gene            complement(14419..14934)
                     /locus_tag="PL162_03770"
     CDS             complement(14419..14934)
                     /locus_tag="PL162_03770"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HQF91176.1"
                     /translation="MPLEKEVLKRLTTLVNSFGLSMIAVCEKLGVGISFNKGKVCVGV
                     NAPGSRVATLISLYGEGAHGTLSIFMSEKCFAFFVHTMTGGMIAPDPEDPVALSVAGE
                     VSNMVSGNALTSLFERGYSGLDITPPQILTGSNIKTLRLPEDTGYTFTLPFLVENDPD
                     RAIYLVLLFRR"
     gene            complement(14985..15500)
                     /gene="thiT"
                     /locus_tag="PL162_03775"
     CDS             complement(14985..15500)
                     /gene="thiT"
                     /locus_tag="PL162_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HQF91177.1"
                     /translation="MERVRLRWLVEGAVVAALSVVLSYLRLWQMPQGGSITLESLPLL
                     LLALWRGPRVGMAAGAVSGLLQLALGGYVVHPVQGVLDYPGAFAMLGVAGFLSRPWWG
                     GFCLGTLGRFCCHVVSGAIFFGSFAPEGSSVWVYSALYNASFTVPSLLLNLALAALLW
                     PKLRALSPSTS"
     regulatory      complement(15558..15665)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            16200..16931
                     /locus_tag="PL162_03780"
     CDS             16200..16931
                     /locus_tag="PL162_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQF91178.1"
                     /translation="MRPLSRTSPLPLYHQLKEELRSLVDDGALKPGDAVPSERELCAR
                     HHISRMTVSKAISTLVDEGVLYREQGKGTFVASPKPSCGFSQLSGFTENMEKAGLSHE
                     TRLLSFDIEEPSRQLQETLQLSPGNKVFNILRRRFVAGEPFSLEQVWLPVDRAPDLSA
                     TLLDGQSLYALLRGRYDVRLSYARQTVEPVLSSAFESGMLELKAETPVLLFCRVAYDA
                     RGEPVEYSKCVYRGVRFKYEMAFYV"
     gene            17013..18398
                     /gene="nagE"
                     /locus_tag="PL162_03785"
     CDS             17013..18398
                     /gene="nagE"
                     /locus_tag="PL162_03785"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-specific PTS transporter
                     subunit IIBC"
                     /protein_id="HQF91179.1"
                     /translation="MSNVFGKVQKLGRALMLPVAVLPAAALLLRLGAGDVLDIPFIMK
                     AGGAVFDNLALLFAIGVAIGLAFDNGGAAGLAGAVGYLTLTSAVVTINKDINMGVLAG
                     MISGITAGVLYNKYHDIRLPEWLGFFAGKRFVPIASSLASIVLALLFGYAWPPIQNAI
                     HNVGEWIVGAGALGAFAFGTLNRLLIPVGLHHVINSLVWFVFGNFTDAAGKVVTGDLS
                     RFFAGDPTAGTFMTGFFPIMMFALPAAAFAMYRTARPENRKAVAGFLFSVAFTSFLTG
                     ITEPIEFMFMFLAPVLYVTHAVLSGAAMAVSNVLGVHCGFGFSAGAIDYLLNWNLATK
                     PFMVIPIGLAFAALYYFLFVAIIKVLNLPTPGRIEQEMVEAPLEEFGGMAGLALAYIE
                     HLGGKGNIQGVDGCITRLRITVRDISIIDDGSFKKLGASGVLRPGKNAIQVVVGTKAE
                     NLADTIRKHMK"
     gene            18570..19718
                     /gene="nagA"
                     /locus_tag="PL162_03790"
     CDS             18570..19718
                     /gene="nagA"
                     /locus_tag="PL162_03790"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HQF91180.1"
                     /translation="MWALTGGKILTPEGFREGWALIYDHRIRAVGREQDLPGGVERLE
                     VRGLWVCPGFIDLHVHGARGVDIMDATPEAIKAMARSLGASGVTSFLATTMTMPLPRI
                     VAALGAIREALGAAPGGARILGAHLEGPFIDPGHKGAQDAAHVLPPDGEFIKAHRDVI
                     RIVTLAPERDPGFRCLDALRASGVVFSLGHSGATFEEALEGVAHGIRHATHLFNAMPP
                     FHHRRPGPVGAALVSDDVTCELIADGVHVHPGLYPLIYRLKGPHRLVLVSDAMRGGGL
                     GEGVWDLGGQQVTVRGGEARLADGTIAGSVLTLDRALRNFREGTGLSLEEMMPLTSGN
                     AARVLGMDHRLGRVAPGYDADLVLLDDEGVVRRTVVGGETIYDAVDGS"
     gene            19784..20509
                     /gene="nagB"
                     /locus_tag="PL162_03795"
     CDS             19784..20509
                     /gene="nagB"
                     /locus_tag="PL162_03795"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HQF91181.1"
                     /translation="MRVLASKDYDQMSRRAAYIVAGQVLLNPRSVLGLPTGSTPQGLY
                     RELVRLFQEGDLDFSEVTAFNLDEYLGLSRTDPQSYAWFMEENLFKFVNVPPERRHIP
                     DGMATDPEGECARYERKIEAAGGIDLQILGLGREGHIGFNEPGVKFEKHTHLIRLTEG
                     TIQANARFFSSPEQVPTRALTMGIKSIMGAKRIILLASGQEKADAVAAAVRGPVSPEL
                     PASILQLHPDVTFVVDGAASSKL"
     gene            20617..21579
                     /locus_tag="PL162_03800"
     CDS             20617..21579
                     /locus_tag="PL162_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91182.1"
                     /translation="MLDLQASDLEKITGRELLASVRAAEGRTLMAETICTLPPLLGDV
                     SNPEAVRAFGADLVLLNMFDVDAPRIEGLPSGGRSGYWTDRYNAAVAPALQGSDPVRE
                     AKTLIGRPVGINLEPLPEDPAVLGVRAVLSPGRTATASSALKARDLGVDFLVLTGNPA
                     TGVTCGAITTALRELRRALGEDYPLMAGKMHAAGSVEESGEGIVTPALVDEWIDAGAD
                     VVLLPAPGTVPGVDVPFVQELVRRIHRRGALALTAVGTSQEGSDEGTIRSLALACKMA
                     GADLHHLGDSGYSGMALPENIMAYSVALRGRRHAYRRMATSPRR"
     gene            21620..22111
                     /locus_tag="PL162_03805"
     CDS             21620..22111
                     /locus_tag="PL162_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HQF91183.1"
                     /translation="MGLFDVFKKKSALELRAPLSGRVVSLDQVPDPVFAGRMVGDGVA
                     IDPTESVVCAPCDGVVAALFSTFHAVGLRTDEGLEILIHVGIDTVAMKGEGFSSAVKM
                     GDRVVRGQELLRFDLDLVRAQAPSTFSPVVLTSMPLVERFQAAEGHVAAGKDVLFSVM
                     LKT"
     gene            complement(22245..22376)
                     /locus_tag="PL162_03810"
     CDS             complement(22245..22376)
                     /locus_tag="PL162_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91184.1"
                     /translation="MKNKIPYEESQNGSLEILEQGSGFPQAFARCAYFDFFCKTLLN"
     gene            22766..23875
                     /locus_tag="PL162_03815"
     CDS             22766..23875
                     /locus_tag="PL162_03815"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HQF91185.1"
                     /translation="MPRDISSVRLNRLNDLLRFFREKESVPRADLFSQLPYTQPRTLE
                     RDLAYLREQFHVQISYDFRRKAYVLKNPGTFVLRMALEEREALALASGLEMVRHFLPH
                     LGEPCHQVWEKLRTMVPSPLARSAEHLARSAVVSLPVSTMDPGHFDAILEAMRRRRVL
                     EARYASPYSHPVEERSYVLSPWGVYFRAHAWYLWAWSHGAEEERTFRVSRFRKVEPSS
                     TPYVPPPEDSGVGDFADGAWYAQGGREGRDVAVRIAPPLSLVVAETRWHTTQSLEAAE
                     DGSVTLRACVPSLDEVAHWILASAPFAEAREPRELREMVRGLAETAGALHRDPVDGKD
                     ATKTDGGDGPRRRGFPPGDGGASGEAFGPEEGSGA"
     gene            complement(24152..24604)
                     /locus_tag="PL162_03820"
     CDS             complement(24152..24604)
                     /locus_tag="PL162_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HQF91186.1"
                     /translation="MAMDALDRNLLADLAADGRISYAELGRKYGLTRVGIKARVEKLR
                     DEGIIERFTVVINPEKIGKNVSAFFEIDVEPFSLDQLAEELSQEDVVENLYLMTGAST
                     LHMHALLEDMGHLERFVLDRIYSKRGIIRVRTHVILRRYKSRRGGARL"
     gene            complement(24717..25109)
                     /locus_tag="PL162_03825"
     CDS             complement(24717..25109)
                     /locus_tag="PL162_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91187.1"
                     /translation="MSLGQPPADPESPDEGAALRKTQLLAICHGNQEIFRGMMEEFLA
                     CVIRAQEELSLPMTLEEAEKVAHRLRGSISYLVEGGATERLRALEEACVTRDSAAVSP
                     AILSAKRSLEALEALLRKELIPGGFPAG"
     gene            complement(25175..25858)
                     /locus_tag="PL162_03830"
     CDS             complement(25175..25858)
                     /locus_tag="PL162_03830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQF91188.1"
                     /translation="MDPIRTLVADDEELSALTHQRFLESVEGFVLVGRAHGGEELISI
                     VQERPVDLLLLDLRLGDAHGLDLLRLLRRDFHAIDAICVSADHATEAIAEAWRLGAFD
                     YLLKPVTFGRFQETLRAYRHYRRLVREVGDVPDQDTIDALRPRSSASSMMLLPKNLQR
                     PTVELFMDFFRRHEETFTAQEVAHRLGLARTTASRYLDFLLHREFLEVSSCPSGPGRP
                     SLLFRRKAP"
     gene            26149..27075
                     /locus_tag="PL162_03835"
     CDS             26149..27075
                     /locus_tag="PL162_03835"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQF91189.1"
                     /translation="MFHGDRARQIWWSDVGLIGIAVFWGSGFALSDVLIQTISPLWLL
                     AARFSLSSLLMGPLLWRRLRGQDLRLLGAASLVGVLLGLTYVVHIYGLVLSTPGKQAF
                     IQSSSVAMVPLLYGLWYGRWPSRPATLGAIVTTVGLFVLGFTPGMLFNAGDALSVLLA
                     FLVALNVMMVGYLSRRMDPLALALAQILSCTALLVVGAWGCEPWPRWGELGTWQWQAL
                     GFLAVFVTVIPFLVQNVAQKYAPEVHAAILLSLESPFGYLIAVFLGRDAWHIQAALGG
                     ALILAGVMVAESERWLPGRKDGTKEKTSGGGA"
     gene            27196..27375
                     /locus_tag="PL162_03840"
     CDS             27196..27375
                     /locus_tag="PL162_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91190.1"
                     /translation="MEESKIRENFVAEVHRAEERVERQKEELARADDEVRRRAAEIVQ
                     ANRKPVVDLGIDLDS"
     gene            complement(27399..28274)
                     /locus_tag="PL162_03845"
     CDS             complement(27399..28274)
                     /locus_tag="PL162_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91191.1"
                     /translation="MAGVLLEAFPSPAPGFMPGMIYVAAWTAGVGGGVALGLLGGQHR
                     YLRRPLLSGRDLRRVVPWALGAGALAGVVAQGAYVLLVSPPTPGESFQWFVEGWRVFC
                     WGLLGLLLGRLLAEAVPNLRRLYGALGGFAGGLLGGVGFVLSSFVMPSGGTRIVGAAI
                     LGAGIGLALTVAERADLARERRALREGVREGEKGARGGEGTRPRPPRPTGLVIHHTPR
                     ERALVKLGPAPVTFGTSPDATVYLRPQGGLKPLSATISLRGGDVVLENHLTGGTRLLA
                     VGDTAQIGPLRVEVV"
     gene            complement(28497..>28690)
                     /locus_tag="PL162_03850"
     CDS             complement(28497..>28690)
                     /locus_tag="PL162_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91192.1"
                     /translation="FARREDFLQAGMDFYALLRERPGEAGPIAVEQALPSWLHEASTQ
                     GSGDDVTVALLFQEAWPVG"
     assembly_gap    28691..28790
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<28791..29497)
                     /locus_tag="PL162_03855"
     CDS             complement(<28791..29497)
                     /locus_tag="PL162_03855"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HQF91193.1"
                     /translation="MRNRHDGGIGPWRALGASVQGASHRRTGRPNQDALGLRILDRGV
                     AVALSDGHGDPASFRSDRGAAFAVEEALDALEDVARRAGGLPFAPLKRAVEEDFSRDL
                     VWRWRHRVEMDQEADPPGDASDPAGSTNLRPYGATVMGALGTAEYLLIFHLGDGDIIA
                     VRDDGTFSPLPPDPRCFANITTSLCLPDAPLDFRTALLPRSGGWPSLLLLATDGYGNS
                     FARREDFLQAGMDFYALL"
     gene            complement(29494..30825)
                     /locus_tag="PL162_03860"
     CDS             complement(29494..30825)
                     /locus_tag="PL162_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91194.1"
                     /translation="MEGSLLKTGQQLRSSLGLPCIVREFLGGGGQGEVYRSSWGDQEV
                     ALKWYFRAQATGEQAAALELLVARGAPDGRFLWPLDLVRHRGVEGFGYLMPLRDPALR
                     GINDIMKRRAEPSFRALATAGFELADSFLNLHARGLCYRDISFGNVFLDPDSGHVAIC
                     DNDNVAVNGTTTTGVLGTPRFMAPEIVRGEASPSTATDLFSLAVLLFYLFMMHHPLEG
                     QRESAIRCLDLPAMNQLYGQDPLFIFDPRDDRNRPDPRYHQNALVFWPLYPSWFRGLF
                     EKSFTDGMGDPLHGRVRESEWRAATLALRDSLVYCASCGAENAPDHPDHPLTCWNCRR
                     PVPLPLRLELGRKTVYLNSDTRLYPWHVRLDPEFDFSRPVGAVGQNPQNPRIWGLRNC
                     SETPWVATFPDGTLRDVPPDRSVRLGPEVRINFGEVEGIVRGAPEVPGGAP"
     gene            complement(30838..31536)
                     /locus_tag="PL162_03865"
     CDS             complement(30838..31536)
                     /locus_tag="PL162_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQF91195.1"
                     /translation="MKRPGGELATRPLHFIWIADTSGSMAADGKMQALNTAIREALPH
                     MQKEADNNPNASVLVRTITFSSGARWVSADPVPVDAFRWQDLAEGGETDLGKALSMVA
                     EQLTMPPMDERALPPVLVLLSDGQPTDAYEKGLQELLALPWGKKAVRIAIAIGGDCDL
                     DVLQRFIANPEIKPLQANNPEALVRYIKWVSTAVVRSASAPASQSPGSAPRGNVPIPP
                     PDSDLGPQDATDVW"
     gene            complement(31621..>33811)
                     /locus_tag="PL162_03870"
     CDS             complement(31621..>33811)
                     /locus_tag="PL162_03870"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQF91196.1"
                     /translation="RLRNLLRDWCPAQRRDVTLVVVAAEERLPALLSSEALAPEARRS
                     LALRHLREERGLDAATAARGVELWTAALEGPRRSPKASHEAPPPETILSPSLSLEDRA
                     RELDQEAWAQAWHFPLSPEGPPEERAMDRAWEALFSPDKGVSPEEAAPLLEEAALQGS
                     EEGRRWLAELQDATGYLPGDIAAVREGRRRRSASDPDEAFRLGWDLIRRQAPSGAVEG
                     RSLLRSSGEQGHPGAWGELGRCLEQGIGGSRSFASAAEAYRRSAEGGDGAAAYALGLM
                     YRAGLGVPRDDREGARWIIQASEAGWAEARSTLGALYSSGAGVPRDFSRAAASYREAQ
                     DLPLLTEEELFLRRSRDFQRRRLDLGDPKALKAWGERLALGWEGPPDRSRALELLDRL
                     EGDLEARSLARRLRGPGAPFPEDPEKEAQELALAPPSAPIRWYRRLLRQYQEKRRLRR
                     READRRAQGGDPEALALMGYLILSGRCGEGQDPGAGGSLLRQAAAAGQEEALAWVGRR
                     YFSPFVPGEAGEGIRDAEDRVLARRWVTREAQRGDREAQRRLGILLLEEDLSPPEGGS
                     DGKERGQGILWLERAAAQGDGEAAFFLGAHWDRQGAAPELRREAERWYRAAAAQGHPE
                     AAYRLALRAFYGKPRDVARAVDLYRQAAERGHAVAQNNLGALYVGGVGVPQDFSRALY
                     WFRRAAERGHEGARRNAEKWAWVEGGEGEGRGLWQRLRSWIDVKNRP"
BASE COUNT         5657 a        10648 c        11384 g         6022 t
ORIGIN      
        1 gtgccgtcga tgtcgatgac ggggacgccc atctggccgg tgcgggccac catgtcccgg
       61 gcggcgtctc ggtcctggct cacgtccacg gaggtgaacg tgacgccttt gcctttgagg
      121 tactctttta ccttggtgca ccaggggcac gtgggggtgg agtaaacttt gacttccatg
      181 gacgccacct ctttcgggat agatgtcttg cctcctccga aagcccggtc gcctcgcccg
      241 agggggggta tgggccttag ttctgtaatg gcagattcac gcgcgggcgg tttcgaaatc
      301 ccgaagaatg gtgtataaca gttcgggcgc tacgggtatc gggagcgatc ggtcctccgg
      361 ggagcgcctg aattatagca caactctagc acaatccgta gggagggagc accatggctg
      421 ttgcaacagc cacaagggag gagctggtcc gacggctcga tcccatcgtg gaaaggcacc
      481 gggggaaaaa gggcatcacc attcctttgc tctccgatgt ccagaaggtt ttcgggtatc
      541 tgcccgagga tgcggtggaa tatgtgggga aggccctcga tattccggcg gccgaacttt
      601 ttggggtggc cacgttctat gccatgttcc ggctggagcc ccaggggaag tacgtggtgc
      661 gcctctgtcg aggcacggcg tgtcatgtcc agggttcggg gcgtatcggc gaacagctcc
      721 agcgcgccct ggagatccag gaagggggca ccaccgccga tggcatgttc accctccaat
      781 acgtggcgtg tctcggctgt tgtagcctgg cgcccgtcat gatggtggga gaggaagtcc
      841 acggccgtct caccccggaa aaggcggtgg aggtgctgga ggggtaccgg gcgaggaggg
      901 tggagccatg acgaaaaccg tggtccgcgt gggggtgggc agctgcggca tcgccgccgg
      961 ggctcttgcg gttctccatc gcctgtcgga ggtcttggga tcccgggagg acgtggtcct
     1021 gaagcccgtg ggatgcgtgg ggctctgttt ccacgagccg ttggtggaga tcgagcggga
     1081 ggggcagcgc tggctctacg gggagatgga tgccgccgga gcggagcggg tggtgcggga
     1141 cttcctcgcc acgggagagg cccccgccga gcatctggtc tacagcaccg tgaccgaggc
     1201 ccccgaaaac gacatgctgg gcaagcaggt gcgggtggtg ctgcggaact gcggcatcat
     1261 cgacccggag aagatcgacg aatatattgc ccgggatgga tacaaggccc tggagaaggc
     1321 cgtacactcc atggcgccgg agcaggtcat cgccgtggtg acggaaagcg gcctccgggg
     1381 gcggggcggt gcggggttcc ccacggggct caagtggaag tttgcccggg aggccaaggg
     1441 ggacttaaaa tacgtggtgt gcaacgccga cgagggagac ccgggggctt tcatggatcg
     1501 gtcggtgctg gaaggcgatc cccacacggt catcgagggc atggtgctct gcgcctacgc
     1561 cgtgggggcc tcccagggcg tggtctactg ccgggcggag tatcctctgg ccatcaagca
     1621 cctgaacctg gccctggagc aggcccggga gcggggctat ctgggcagga acattctggg
     1681 cacgaccttc tccttcgata tctacgtcaa agaaggggcg ggggccttcg tctgcggcga
     1741 agagacggcc ctcatcgctt ccatcgaagg aaagcggggc atgccccgtc cccggccccc
     1801 cttccccgcc cagagcgggg tcttcggcaa gcctacgaac atcaacaacg tggagacctt
     1861 cgccaacatc ccctggatcc tggtgcacgg cgccgaggcc ttcaaccgct atggcatcgg
     1921 caagagccgg gggaccaagg tcttcgccct ggcgggaaag atcgtcaagg gcggtttggt
     1981 ggaagttccc atggggatgc cccttcgcga agtcattttc gacgtggggg gaggggtccc
     2041 cgacggcaag acgttcaagg cggtgcagat gggggggcca tcgggggggt gcattccggc
     2101 ggctcttctg gataccccgg tggactacga gtccatcaac gccacggggg ccatcatggg
     2161 ctccggcggc atggtggtca tggacgaggc cacctgtgtg gtggacgtgg ccaagttctt
     2221 cctctccttc acccagcggg aatcctgcgg caagtgcccc ttctgccgca tcggcaccaa
     2281 gcggatgctg gagatcctca cccggatcag cgaggggcag gggcgtcccg acgacgtgga
     2341 tttgctcctg gagatgagcg tccagatcaa ggacggctcc ctctgcggcc tggggcagac
     2401 cgcccccaac ccggtgctca ccacggcgaa gtacttccgg gaagagtacg aggcccacat
     2461 ccgggatcac cagtgtcccg ccaaggtttg ctccgctctc attcactaca ccatcgacgc
     2521 ggaaaagtgc atcgggtgca ccaagtgtgc ccgggcctgc ccggtgaagg ccatcagcgg
     2581 cgctctcaag gcgccccacg tcatcgacga cggggcgtgc atccggtgcg gtctgtgcct
     2641 caagagctgt ccggtgaaag ccatctccgt ggagtaagga ggttctcgaa gagccatgaa
     2701 ggaacgtacg atccgagttc tgcttgacgg gcgggaggtc ttcggatttc ccggccagag
     2761 gctcctggat ctctgcgcgg agtgtggcgt ggagattccg gcgctctgtt acgaccccca
     2821 cctgtccatt cacggcggct gctccatctg cctcgtggag gtgaaggggg ccaaggccct
     2881 ggcccgggcc tgcgccacca ccattgggcc ggacatggaa gtgaccaccc ggagcgaacg
     2941 ggtgcgaaaa gcccgcaaac tggggctgga acttcttctg tcggaccacg tgggggactg
     3001 ccgtcctccc tgcaccctgg cgtgtcccgg tcggggcgat gtgcaggggt atgtgaacct
     3061 ggcggcccag ggcaagtggg aagagtccct cgaagccctg cggcggaaca tcacccttcc
     3121 cgcgtccatt ggccgggtct gccccgcccc ctgtgaaaag aagtgccgcc gcaatctggt
     3181 ggacgacgcc cccgtgtcca tccgggagat caagcgggtg gtgggagacg tctgcctcca
     3241 cgagggcacc atggggcaca ttcccgagat cgtggaaaac ggccagagcg tcgccgtggt
     3301 gggggcgggt cctgcgggcc tttcggcggc cctcttcctc cggcgcaagg gatacaaggt
     3361 ggacctctac gacaaggaac ccctcttcgg cggcatgatg cgctacggca ttcccgatta
     3421 ccgtctgccc caggacgttc tccaggccga ggtggagtgg atcctcgcca acggagtgac
     3481 ggcccatccc aataccgccc tgggccggga cgtgtctttg gatgagctcc gggaaagcca
     3541 cgacgccgtg ctcctcgccc tggggtgctg gaagtcctcg ccgatgcgct gcgccggcga
     3601 gaacctcccc ggcgtgtggg ggggcatcaa cttcctctac aaggtcaacc gccgggaacc
     3661 ggtggcggtg gggcgccggg tggcggtggt gggaggcggg aacaccgcca tggacgcggc
     3721 ccgatgcgcc cgacggtacg gggcggagaa ggtcttcgtg gtgtaccgcc ggacccaggc
     3781 ggaaatgccc gccgagccgg tggaaattca ggaagccatg gaagagggcg tggagttcct
     3841 ctttctggcg gctcccaagg ccgtggaagg ggaaggccgg gtggagcgca tggtgtgcga
     3901 gaaaatggcc ctgggagacc ccgacgcttc ggggcgccgg agccccgttc ccacgggaga
     3961 gaccttcgtg ctggaggtgg ataccgtggt ggccgccatc ggccaggcgg cggacttctc
     4021 cgcccttccc aaggtgctcc atgacggtcg gcggcccttg gtggacgaga actacgccac
     4081 accccttccc ggggtctttg ccacgggaga ttttcagacc ggccccgata tcgccgtggc
     4141 ggccatgggg ggcggacact gggcgtcgga atccatccac gcctggctca ccacgggggt
     4201 ggcgaaacgc cccttcgagt acgacgtggt gcaaaaagat ctcaccagcg ccgatttcgc
     4261 ccacgtggag cgcacgcacc aggagcatcc gcaccacgat gcccccgagg cgcgcctggc
     4321 gccggatcgg gcttttcagg agttcaacca cggcctcacc cccgaacagt gcaagaccga
     4381 cgcggcgcgc tgcatggagt gcggttgcgg cgacctcttc gagtgcaagc tccggcgata
     4441 tgcccagcag tacgaggtga accccgaccg ggtggcggga gcccacgtgg cgggagttca
     4501 ggaagtgaac agttcctatg tccggaacat ggacaagtgc atcctctgcg gccgctgcgt
     4561 ccgggtctgc gacgaactgg cggggttcca cgccatcgac ttcacccggc ggggctttga
     4621 aggaagcgtg gatgcggcgt tctggcgcgg caccgagggg accgagtgca cccagtgcgg
     4681 gctctgcgtt cagctctgcc ccgtgggggc cctcatggag aagcgcgctc cccggaaacc
     4741 ccacagcgag attccccaga tcatgaagac cgcctgcggg cgttgcggcc ttgggtgtca
     4801 gctggagctc aatctggatg cggatcggcg ccgggtggtg cgggtcacca cggacttcga
     4861 cgatcccacg tccgtgtccg gaggaaacag ctgcgcccgg ggacgttttg gcgacgaagc
     4921 cgtggcggaa aagcgcctcg ccgaaccccg gttccgggga gaggaagggc gctggaacac
     4981 ccttctggac gacctcggcg gaaaactcaa gggcgttctg gacgcctcgg ggggctctgc
     5041 ggtggcggtg gtggcggggg gaagcctggg caacgaggaa tatctggccc tccagcgtct
     5101 cgtgacggaa gccctgccgg gagctctgct ggcggtggaa ggggtggacg ccttcgcccc
     5161 cgcccgacag gttctggaaa cggcgtggaa gacccccctg ggcggagcga cctacgccga
     5221 cgtgcggcac gccgacgcct atctcctgtt ggaaagcgac accgaccgga cccatccggt
     5281 gctcacctcc tggatgcggg gggccatgcg gaaaaaccat gcggcggtgg tctacatcgg
     5341 ccaggatgcg ggaaagctgg accggggcga cgccttggtc ctgtcccccc gggagggcac
     5401 ccgagaggat cttctccggg gcctttccgt ggcggcgctc caggctgcgg gccggaccgt
     5461 ccccgacggc tgggcggagt acacgcccct gaagaccgcc gccctcaccg gggtggacga
     5521 gaagatcatc gagggagcgg cccatgccct ggccaaggcc aagagattgg tcaccttggt
     5581 gggggccccg gtggggtctt ccggcggagc catggagatc gtcaccaccc ttctggggat
     5641 cctgggcgcg cgtcgggtga tgttgttgca cgaaggagcc aatgtggtag gggcggcgct
     5701 tcttaatgtg gcgtccgcct cggtggcgga cgtggtggga gccctccgag ggggggacat
     5761 caaggccctt ctcaccgtgg gagtggaccc ggcggccctt ggaatgaacg atgacgacct
     5821 gaagggcctg gcgatatcgg cggccctgtc cacggagcgt ctgaccacct cgccggacta
     5881 cgtgcttccc atggcggact ggcgtgagag agaaggccgg gccttcaccc tggcgggggt
     5941 gcaggtggag caggtccggg ctattcccgc tccgggcttg gcggtggagc tctgttggac
     6001 cgtggcggcc ctggcccgga ggttgggccg ggagatcccc gtccggatcc ccctggtttc
     6061 cgccgggagg taggccgccg tagcgtgagc accgtcgccc ctttcgggcg atctttgggt
     6121 cggggcggcg aaaggagccc cggccctcct tttatccgac gaaaagatgt gagagaatgg
     6181 catcccctgg aggggagcgg cgaccgggcg tttcggagtc gagggcgttc ggggccgtca
     6241 ttttgttgtt ctttggagga gagcgaagat gtcaggcagt cagcaacact cgtcggtgcg
     6301 cattgtggtg gcggatccct cggcgctggg ccttttcggt ttggccatgg tcaccctggt
     6361 ggcctcttcc cagaaactgg gccttaccac gggactttcg ttcgtcgtgc cctgggccct
     6421 cttcttggga tctttggccc agctcatggc ctgcgtctac gatttcaaac acgggaacac
     6481 ctttggggcc acggcgtttg gggcctacgg tctcttctgg gctggcgtgg cggcgtcatg
     6541 gctcatccgc atgggggtct tcgggccgga gctggcggcg gaagtggacc cccgggagct
     6601 gggggtggcc tttttggggt acaccatctt ctccatctac atgaccatcg gcgccatgga
     6661 aacgcacaag gtcctttttg ccatcttcgt gctcatcgac gtgctcctgg gggccttggc
     6721 ctacaacacg ttctaccccc agagctccgg agttcacgcc ctggcggcct ggagcgagct
     6781 ggccatctcc ctgctttctt tctacggttc cgccgcctca gtgctcaata gccacttcgg
     6841 ccgccccttc gtcccggtgg ggcgcccctt cggatggttc aagtgatccc aagggttaaa
     6901 gaatagatgg gtcacgacgg tcccccggaa gatcaacttt tctttcgggg gaccgattgt
     6961 ctttacgggg gttttgcgct acactcgccc cagacgaggg taggtaattc aagctaggca
     7021 aagtagcgga tctccagcag agcataagac cagctttgga ggttacaggt atggttaagc
     7081 gcatgcagcc cgagcccttc cgcatcaaaa tggtggagcc cgtctccgtt cccgacgccg
     7141 ccgcccggga gaaggccctg gaggcaggac atttcaacat gttcggcctt cggtcggagg
     7201 acatttacat cgatctcctc accgactccg gcaccggcgc catgagcaag taccagtggg
     7261 ccgccctcat gaagggagac gaggcctacg ctggggcgga cagcttctac gccctgaaaa
     7321 atgccgtcaa ggaagtgctg ggcttcgatt acgtcatccc cacccaccag gggcgggcgg
     7381 cggagcacgt ggtcttcagc accctggtga agcccgggaa tatccttccc ttcaacatgc
     7441 ccttcgacac caccctggcc catgccttca acgccggcgg cattaccgtc aactgtgtca
     7501 tcgacgaagc cttcgacccg gagagcctgc ttcccttcaa gggcaacgtg gacatcgcca
     7561 agctcgaagc caccatcgcc cagcacggcc gggacaacat ccccttcatc atggtcacca
     7621 tcacgaacaa ctccggcggc ggccagcccg tgagcctgga gaacctgcgg gcggtgtccg
     7681 ccgtggcgaa aaagcacggc ctgcccctcc tcctggacgc ggcccgcctg gccgagaacg
     7741 cctacttcat caaaatgcgg gagcccgcct gcaaggacat gaccgtggcc cagattctca
     7801 aggccaccat ggacacggcg gatggcatca cctgctccgc caagaaagac cccctcgtga
     7861 acatcggggg gatgatctgc tgccgcacgg aagagatgta ccacgccctt ctcccccggg
     7921 tgatcctctt cgagggtttt gtcacctatg gcggtttggc ggggcgcgac ctggaatgcc
     7981 tggcccaggg gctccgggag atggtggacg agcactatct ggcccaccgc gtgggccagg
     8041 tgaagtatct gggagacctt ctggaagagg gaggggtgcc cttcgtgaag cccgcaggag
     8101 ggcacgctat tttcatcgat gccgccgcct tccttcccca cattccccag aagtactatc
     8161 ccgccgacgt gctggccatc gaggtctacc gcgagggcgc cgtgcggggc atcggcctgg
     8221 gggccctggc cttcgccacc gaggacgaga agaccggcga ggtaaagatg cccaagctgg
     8281 agctctaccg cctggccatc aaccgccgga cctataccaa tagccacatc gagttcatcg
     8341 cccagaccat cgtggacgtc tacaagcgtc gggagagcat ccggtacggc ctgaaggtgg
     8401 agtacgcccc ccccgtgaag ggcatccacc acttcctggc ccatctgcgc cctttcaacc
     8461 tgtaagaagg gcgcccgggg cgggggtgtc aaaagagccg gggagccccc ttcgaggctc
     8521 cccggcttca tcgtgggcgc aggcgcaggc acggcctcga tctctagccc ctagcccatg
     8581 cgttggagct cttcttcggt gaagtctccc cccagggggg cgtttttgat caggtcttcc
     8641 aagacctttt tcggctttcc tcggttgaat aggacctggg ccaccgcctg ggccagggta
     8701 caaccctggg ccgccagcgc ccgggcggtg tggtaccctt ccaccgtgtg ttttatttcc
     8761 gccagggcgt cctcggggga cttgtcctgc cccagcagga ctcccatgcg aaactcccgg
     8821 gagttctcgg tgcagaagcg ccacaggtcc cccgcccccg ccaggcccag cacggtgccc
     8881 gggacgccct tcatgttccg agagcgacgg gcgatctccg ccatgcccag ggtgatgatg
     8941 gcggtcatgg cggcgtcccc caggccgagc ccccggacca tgcctgcggc gatggccgtg
     9001 acgctgttgg ccatggtggc ctccacccct cggacgtctc gggaaatgaa aactcgaaaa
     9061 cctggagtgc tcagtaggac ttgccactcc agggcctcct ccgtcaggga agaggccacc
     9121 accagggcgg tggcgtgtcc ctgggccacg tcttcgggaa aggcgggccc tcccaagacg
     9181 ctgtagaccg ccccggggat gacctcgtga cagagggcgc tccccgtgag ccctgtgagg
     9241 gcctccatcc cccgggcggc gttgcacacc cggagatcgt ggggggccag gggtttgagg
     9301 tgttgccaga tctcccgcag ggcctgaaag ggcacggcgg cgatccatcg tttgccgaaa
     9361 acggccgctt cggcgaggct cccggtggcc tcgacatttg aggggagggc gagctccggg
     9421 aggtagtccg ggtttcgccc tgtggcgatg atggctcggg cctgcggggc ccgacggcac
     9481 cacaggcaaa cccggtgacc gttggaggcg gccaccgaag cgagggccgt tccccaatcg
     9541 cccgcgccga ataccacgat atgggccatg ggcgttctct cctttcgttt cacttcgtcg
     9601 tggagaagaa agacctcctc gtgccattat agggctccga ggcgagggcg caacccgggg
     9661 ccggtataaa tactttgcgc cggggagttt ttttcgtaag attatcgaag aaaagggtgg
     9721 ctcggcgagg ccgccatact ttctggaggt gattatcctg ttacagcatg agcatcccgc
     9781 catcaggacc ctgctggggc gccgaagcat ccggcgcttt gaaggccgtc cggtgccgga
     9841 ggacgcggtg gcgctgattt tggagtgcgc ctgtgcggcc cccagcgcgg cgaacaaccg
     9901 cccctggcac ttcgtggtgg tggacgaccg gagcaagctg gatgccctgg cgggggtttt
     9961 gccctacgga aaggcgctat tccaggcccc cctggcggtg gtggtgtgcg gagatcccac
    10021 caaaagcgac atgtcccggg cgtactggga ggaagattgt tccgccgcca tggaaaacat
    10081 cctggtgggg gcctgggccc tggggatcgg gagcgtgtgg ctcggggtac accccgtgcc
    10141 ggggaggtcg gaggcggtgc gccagatctt ggacatcccc ggatccgtgt cggtcctggg
    10201 catcgccgcc ctggggtacc ccggcgagga aaaggggccc cacgcgggcg tggatcccgg
    10261 ggtggtgcac cagaacagat ggtaggtggc tttttctcca tcggcggccc tgttttgcca
    10321 ggttagaaag cacggcgatc ctggggcgct tcggcgacgc cgtccactct tttcgggcgc
    10381 tcatccatga tcccatgagg tataatgggc gagcgccccg ccgataggcc cgcgttctcc
    10441 ctcgaacggg gagatctcgg cggggaggcg tcacctgagt gagcttgccg aggaggaacg
    10501 ggaatggccg aacaaatggg agggcagctg gagttcagca aggtggtgcc gctgccgctc
    10561 gaggaagaga tcaaacatag ctacctggat tatgccatga gcgtcatcgt ggggagggcc
    10621 cttcccgacg cccgggacgg gctcaagccc gtgcagcgcc gcatcctctt cgccatgctg
    10681 gagctggggc tccggcacaa tcagcccacc aagaaatccg cccgcgtggt gggagagacc
    10741 atgggtaagt accaccccca cggcgatcag gccatctacg acgccgtggc ccgcatggcc
    10801 caggacttta gcctccgtta ccctctggtt tccggtcagg ggaacttcgg gtccatcgat
    10861 ggggaccccc cggcggccat gcgatacacc gagtgccgtc tccatgccat gggcgaagag
    10921 atcctggcgg atctcgacga agagaccgtg gactgggggc cgaacttcga cgactccctg
    10981 aaggagcccc tggtgctccc ctcggccctg ccgaacctcc tggtgaacgg ggcctcgggc
    11041 atcgctgtgg gcatggccac caacctcccc cctcacaacg tggaggaggt ggtggacgcc
    11101 ctgtgctacc tcatcgacca cgaagacgcc gagctggggg agctcatgca gttcctcccc
    11161 ggccccgatt tccccacggg gggaatcatc atggggcgag acggcatcat cgacgcctac
    11221 cgcaccggcc gggggcgcct cctggtgagg ggcaaagccg aggtggagga gcgccaccac
    11281 gggcgccggg ccatcgtgat ctccgaaata ccctaccagg tcaataagac taacctcatc
    11341 gagagcatcg ccaaaggggt ccagaacaag gccctggacg gcatttcaga cctccgggac
    11401 gagtcggacc gccaggggtt gcgcatcgtg ctggagctcc agcgggacgc cactcccgag
    11461 gtgaccctgc gccaactcta ttcccggact cccctccaga ccactttcgg ggtgattaac
    11521 ctggctttgg tgaaggggga gccccgggag ctccccctcg tggacatgct gtccgtcttt
    11581 ctggcgcacc gtcgcgaggt ggtgcgccgt cgcaccgagt tccgcctccg caaggccgag
    11641 gagcggtccc atctcgtgga gggcctcctc aaggccctgg acatcatcga cgaagtcatc
    11701 gccctcatcc ggggatcccg ggacggagag gaggcccttc gaggcctggt ggaccgtctc
    11761 ggattcaccg aactccaagc ccgggccatc ctggagatgc gcctccagcg tctcacggga
    11821 ttggagcgca ccaagctcga agaggagagg gcccagttac tggcggccat cgaaggcttc
    11881 cgggcgatcc tcgcccaggc ccaggtgcgg gatcaggtca taaaagacga gctccaggag
    11941 attagtaagc gccaccggga ttcccggcgc acggagatcc aagacggcat ggaagacgtg
    12001 gcccaggagg atcttatccc ggaaaaagac atcgtggtgg tgttgagccg ggacaacttc
    12061 ctccggcgca tgcccctgga agactatcgc cgtcagcagc gaggaggaaa gggcgtcaag
    12121 ggagcccccc ccaaggaaga ggatgaaata tccatcgtgg ccgttaccac cacgcacagt
    12181 ggcctctatc tcttcacctc caaggggagg gttttttccc ttcggggcca cgtgatcccc
    12241 cagcccaaga ctggcaaggg gcgccacgtg agcgcctttg tccccctgga agaaggggaa
    12301 agggtgatga ccctgcggga tgggcgcatg gcggggtctc gatttgtgtt ttttatcaca
    12361 aaaaggggcg tggccaaacg aatgccggtg acggagctgg aaaacatcac ccgggcgggg
    12421 cgccgggtgc tgtctttgga cgagggagat gacatcgccc gggtccggtg caccacgggg
    12481 gaagaagatc tccttctggt caccgcccag gggcaggccc agcgggtgaa cgaggaagag
    12541 tttcgcccca tgggacgcat ggctcgggga gtccggggca tccgcctgga cccgggggac
    12601 gaagtggtgg ggtgcgacgt ggtccgcccc ggtgtcacgt cccttttcct gagcgagaac
    12661 ggcattggca agcgcacgga gtacggtgag ttcaccgtcc accatcgagg gggcgggggg
    12721 gtgcgctcca tgcgtctggg ggataagacc ggatccctcg tcggatcgtg gagcgtccac
    12781 ggcggcgacg agattttggt catttccgcc cggggtcgca tggtgcgcct gggggtggag
    12841 gacatccccc tcctgagtcg caccgccaca ggccccatcg tggtgagatt ggacgaaggg
    12901 gatgcggtgg tggatgtgag cgtcttttcg agcgctcccg aggacggaga atgaggcccg
    12961 tggcccggga cggttttccc ctgatcggtg gggtggccct ggcctgtgcg gccggggggg
    13021 tctacgacgt tcacctcgcc ctggggattt tgccccttct ggctctggtg atctggtttt
    13081 tccgcgaccc cgagcggacc accaccgccg ggcccgggag cttcgtgagc cccgccgatg
    13141 gcaaggtggt cgaagtgggt tcggcgttcc atccctttgt ggggaacgcc acccgggtgg
    13201 ggatcttcat gtctcccctc gacgtccacg tgaaccgggc gccttttgcg ggcatcgtgg
    13261 cccacttgga gtacgttccg ggggcccgat ggatggcctt cgaccccaag gcctcggagc
    13321 ggaacgagcg ctttttcgtc ggactggaca cggcggtggg acctgttata atcacccagg
    13381 tagctggatt tctggcgcga cgcatcgtct gtcggctgat tcggggggat cgcctggagc
    13441 ggggagagcg ttacggcatg atcaagctgg gatcccgggt ggacctgtac ctccctccgg
    13501 cggtggagtg cgtggtcgcc gtgggccaga gggttagggc cggcgagacg acgataggag
    13561 tggtgagaga tggccctgag acgcaaggag cgtgaagtgc ccttcaggaa gattatcccc
    13621 aacatgatca cgtgcggcaa cgtgctctgc ggcatgctct ccgtggtgtt gagctttcac
    13681 gagcattacg ccctggcggg gtggctcatc ctggcggcgg ttttcttcga tttcatggac
    13741 ggcaaggtgg cccgaagtct gggggggagc agcgccttcg gcatggagct ggatagcctg
    13801 gcggatgtgg tgagctttgg cgtggccccc gccatggtgg tctatggcgc ggccctccgg
    13861 ggattcctcg gggtcacggg agccctggcg gcggcgtttt ttgccctctg cggcgctctt
    13921 cgcctggcga ggttcaacgt tacccacgcc ccggcgggat cgttccaggg attgcccatc
    13981 cccgccgggg ggctctttct cgtctccttc gtcatggggg gagtgaccct tccgccggcg
    14041 atcttcgcgg ccgtggtggt gggcacgggg gtgctcatgg tatccaacat cccctacggg
    14101 aacttaaaaa aactccgcaa gggaaacatg aaccgtggca aggggctctt tctgtccctc
    14161 ttcctcctgg ggctgggggc gattttccgg gagaaggccc cccttatggc catgggcatt
    14221 tacgtggtga gcggcttctt ccgctttgac tggggcggat ggctgtccaa gaaggagggg
    14281 gagtgccccg ctcccgggga aaagccctag ggtttttatg tttttggtcg agcgccgggg
    14341 aaagaaaaaa ggagcccttc gagggctcct tttcgtcgtg accgtcccgc gtgaccgtcc
    14401 cgcccctggg gggatctcct agcgccggaa gagcagcacc aggtagatgg cccgatcggg
    14461 gtcgttttcc accaggaagg gcaacgtgaa ggtgtagccc gtatcctccg gcaggcgaag
    14521 ggtcttgatg ttgctccccg tgaggatctg ggggggggtg atgtcgagcc ccgagtaacc
    14581 cctctcgaaa agggaggtca gggcattgcc gctcaccatg ttggacacct cgcccgccac
    14641 cgaaagggcc acgggatcct ccggatcggg ggcgatcatc cccccggtca tggtgtgcac
    14701 gaagaatgcg aaacactttt cgctcatgaa gatggacagc gtgccgtggg ccccttcgcc
    14761 gtagagcgat atgagggtgg ccacccggct tcccggggca ttgaccccca cgcacacctt
    14821 ccccttgttg aaagatatgc cgacgcccag cttttcgcac acggcgatca tgctcaggcc
    14881 gaaggaattg acgagggtcg tcaaccgctt aaggacttcc ttttccaagg gcaaggggcg
    14941 ttcgctcctt ccaagagtga tacgtctttt ccccggggcc gcgatcagga cgtggagggg
    15001 gacagggctc gaagcttggg ccacagcaaa gcggccaggg cgaggtttag cagcagcgac
    15061 gggaccgtaa agctggcgtt atacaaggcg gaatagaccc acacggaact cccctcgggg
    15121 gcgaagcttc caaagaaaat ggcccccgac accacgtggc aacagaaccg gcccagggtg
    15181 cccaagcaaa accctcccca ccacgggcga gagaggaacc ccgcgacgcc caacatcgca
    15241 aaggccccgg ggtagtctag cacgccctgg accgggtgca ccacataccc ccccagggcg
    15301 agctgcaaga gccccgacac cgcccccgcc gccataccta cccgggggcc gcgccacagg
    15361 gccaaaagca ggagaggcaa ggattccaac gtgatggatc ccccctgggg catctgccac
    15421 agccgcagat aggaaaggac caccgacagg gccgccacca cggcgccttc caccaaccac
    15481 cgcaaccgca ctctttccat gttgcctccg cctccaaatt ccggtggtac gaggggaggt
    15541 cggatagaaa gatccgagga ccgcgccatg cccatccctc cgccggtacg aaccggatca
    15601 ggttccaggg gtcgggatgt taagatcccc tctcagccgc cttcgcggct cccccagggc
    15661 cacccccgct tatactccct cgagggtgga tgtgtcaagc tacgcggccc atctcaaatc
    15721 ctcgatgtgg gcgcttgcct atgatctcgt tcgccaactc cgacaagttc gacctatgcc
    15781 aaagctcgac agccggaagc aagccccctc gacttgtcag gtttgcccgc tgattccgct
    15841 ctatttcgta gtgacagttc ccttgttttg cccccataat atacgcaccc ctttggtgtg
    15901 aggttttggg tctgcgcctt aaccttactt ccgctgttca agcggcacca gaggggtttg
    15961 cggatcaagg cccatccccc ctccggcccc gatcccaatc gtcctgatcg aaggggcccg
    16021 gagagcttta ccctcggctc ccgaaatctc tgccccccgc cccgccggag gggataggcc
    16081 gatggattct ttcccccttc gacccccctt gacatggaga gaaagagggg tatagtgggg
    16141 ccataagtgg tctagaccac atgtcaacat aaggactcta tctccttcgg aggtgtgcgg
    16201 tgagacccct ttctcggacg agccccctgc cactctacca ccaactgaaa gaagagcttc
    16261 gaagcctggt ggacgacggc gccttgaagc ccggcgacgc cgtgccttcg gagcgggagc
    16321 tttgtgcgag gcatcacatc agccgcatga cggtgagcaa ggccatctcc acgctggtgg
    16381 acgaaggggt gctctaccga gagcagggca aggggacctt tgtggcgtcc cccaagccct
    16441 cgtgcggttt ttcccagctt tcggggttca cggaaaatat ggagaaggcg gggctatccc
    16501 acgagacgcg actgctctcc ttcgatatag aagagccctc tcgtcagctc caggagaccc
    16561 tccagctctc tcctggaaat aaggtcttca atatccttcg gcggcgcttc gtggccgggg
    16621 agcccttttc cctggaacag gtctggctcc ccgtggatcg ggctccggat ctcagcgcga
    16681 cgctgctgga tggtcagtcg ctttatgcct tgctccgggg caggtacgac gtgcggctct
    16741 cctatgcccg ccagaccgtg gagcccgtgt tgagctcggc cttcgagagc ggcatgttgg
    16801 aactcaaggc ggaaacgccg gtgctcctct tttgccgggt ggcctacgac gcccgggggg
    16861 agccggtgga gtacagtaaa tgcgtctatc ggggggtgcg cttcaagtac gagatggctt
    16921 tttatgtcta gcgtggcggg gcgccggggg gcgccggcgg gccggggggg agatcccttc
    16981 cgaagggtgc gatgcgtaag gaggaatggg ctgtgagcaa cgtgttcggt aaggtgcaga
    17041 aattggggcg ggccctgatg ttgcccgtgg cggtgctgcc ggcggcggcg ctgctgttgc
    17101 ggctcggagc cggggacgtg ttggatatcc ccttcatcat gaaggccggt ggtgcggtgt
    17161 ttgataacct ggcgctgctt ttcgccatcg gtgtggccat tgggttggcc ttcgacaacg
    17221 gcggcgcggc ggggctcgcc ggagcggtgg gatatctcac cctcaccagc gctgtggtga
    17281 ccatcaacaa agacatcaac atgggagtgt tggcggggat gatttcgggc atcaccgccg
    17341 gagtcctcta taacaagtac cacgacatcc gcctgccgga gtggctcgga ttcttcgcgg
    17401 gaaagcgctt tgtccccatc gcctcatccc tggcgtccat cgtgctggcg ctcctgttcg
    17461 gctacgcctg gccccccatc cagaacgcca tccacaacgt gggagaatgg atcgtgggag
    17521 cgggggccct gggggccttt gccttcggca ccctgaaccg cctcctgatc cccgtggggc
    17581 tgcatcatgt catcaacagc ctggtatggt tcgtcttcgg aaacttcacc gatgcggcgg
    17641 ggaaggtagt cacgggagat ctctcgcgct tttttgccgg agatcccacg gcggggacgt
    17701 tcatgaccgg gtttttcccc attatgatgt ttgccctccc ggcggcggcc tttgccatgt
    17761 accgcaccgc ccggccggaa aaccgcaagg ccgtggcggg gttcctcttc agcgtggcct
    17821 ttacctcctt cctcacgggc atcacggaac ccattgagtt catgttcatg tttttggcgc
    17881 cggtgctcta cgtcacccac gccgtgctct ccggagcggc catggcggtg agcaacgtcc
    17941 tgggggtgca ctgcggcttc ggcttctcgg cgggggccat cgactacctg ctgaactgga
    18001 acctggccac caagcccttc atggtcatcc ccatcggcct ggccttcgcg gcgctgtact
    18061 acttcctctt cgtggccatc atcaaggttc tgaacctccc cacccccggg cgcatcgagc
    18121 aggagatggt ggaggcccct ctggaggagt tcggcggcat ggcggggttg gcgctggcct
    18181 acatcgagca cctgggaggc aagggaaaca tccagggagt ggacgggtgc atcacccggc
    18241 tgcggatcac cgtgcgggat atttccatca tcgacgacgg gagcttcaag aagctggggg
    18301 cctccggggt cttgcgcccc ggcaaaaacg ccatccaagt ggtggtgggt accaaggcgg
    18361 aaaacctggc ggacaccatc cgcaagcaca tgaagtagcg gtcgatccct tgccccggga
    18421 cccccctcgc ccttcgaggg atcccggggc tttttgctgt gatcccggga tctcggggct
    18481 tgtgtttatg gaggaccttt cggacaatgg aggcggtgcc gcagagcttt ccggaaaagc
    18541 cgaaggcgaa aaggagggag agagaggcca tgtgggcgtt gacgggggga aagatcctga
    18601 ctccggaggg ttttcgggag gggtgggccc tgatttacga ccaccggatc cgggccgtgg
    18661 ggcgagaaca agaccttccg gggggcgtgg agcgtctgga ggtccggggt ctttgggtct
    18721 gccccgggtt catcgacctt cacgtccacg gcgctcgggg ggtggacatc atggacgcca
    18781 ctcccgaagc gatcaaggcc atggcccggt ccctgggggc ctcgggggtg acgtcttttc
    18841 tcgccaccac catgaccatg cccctgcccc ggatcgtcgc cgccctaggg gcgattcgcg
    18901 aggccctggg ggcggccccc gggggggcac gcatcctggg ggcgcacctg gagggcccct
    18961 tcatcgaccc cgggcacaag ggggcccaag acgccgccca cgtgcttccc cccgacggag
    19021 agttcataaa ggcccatcgg gacgtgatcc gcatcgtgac cctggccccc gagcgggatc
    19081 cgggcttccg gtgcctcgac gcactccggg cctcgggggt ggtgttctcc ctggggcact
    19141 ccggcgccac cttcgaagag gccctggagg gagtggccca cggcattcgc cacgccacac
    19201 accttttcaa cgccatgcct ccctttcacc accgtcgccc ggggcccgtg ggggccgcct
    19261 tggtgagcga cgatgtcacc tgcgagctca tcgccgacgg cgtgcacgtc catccggggc
    19321 tctaccccct gatctaccgc ctcaagggcc cccaccgcct cgtgctagtt tccgacgcca
    19381 tgcggggcgg gggcctgggg gaaggggtct gggatctggg aggacagcag gtgacggtgc
    19441 gaggcgggga ggcgcggctg gccgatggca ccatcgccgg aagcgtgctc accctggata
    19501 gggccctccg aaacttccgg gaggggacgg ggctttcttt ggaagagatg atgcccctga
    19561 cctcggggaa cgccgcccgg gtgctgggga tggatcatcg tttggggcgg gtggccccgg
    19621 gatacgatgc agacctggtg ttgctggacg acgaaggggt cgtgaggcgt accgtggtgg
    19681 gaggggagac gatctacgac gcagtcgacg gatcgtgagc ccccttttag cgcgaggagg
    19741 gatccttcct cctccgacgt gatccgaagg gtgaggagac tttatgcgag tgctggcgtc
    19801 aaaagactac gatcaaatga gtcgtcgggc cgcctacatc gtggcgggac aggtgctttt
    19861 gaacccccga tccgtgctag ggctgcccac ggggagcacc ccccaggggc tctaccggga
    19921 gctggtgcgc ctgtttcagg agggagacct ggacttttcc gaagtcaccg ccttcaatct
    19981 cgatgaatat ctgggacttt cccgcaccga tccccagagc tacgcctggt tcatggaaga
    20041 aaatcttttt aaattcgtga acgtccctcc ggagcgccgc cacatccccg acggcatggc
    20101 gacggatccg gaaggggagt gcgcgcggta cgagcggaag atcgaggccg ctgggggaat
    20161 cgatctccag attctcggcc tggggcgaga gggacacatc ggttttaacg aacccggcgt
    20221 gaaatttgag aaacacaccc atctgatccg gctcaccgag gggaccatcc aggccaatgc
    20281 ccgctttttc tcctcgccgg agcaggtccc cacccgggcc ttgaccatgg gcatcaaaag
    20341 catcatgggg gcgaagcgca tcatcctgct ggcctcgggc caggaaaagg ccgatgcggt
    20401 ggccgccgcc gtccggggcc ccgtgagccc ggagctcccg gcgtcgatcc tgcagctcca
    20461 ccccgacgtc acctttgtgg tggatggggc ggcgtcgtcg aaactatgac atatcggagg
    20521 ggcccctatc cccagggggc gcctttgatg cccccgatcc gcgccagggg tgaaaggcca
    20581 tgggggaaca aagaaggagt gaggagcgtg cgacgaatgt tggatctcca ggcctccgac
    20641 ctggaaaaaa ttaccgggcg tgagctgttg gcgagcgtgc gggccgccga ggggcggacc
    20701 ctcatggcgg aaaccatctg caccctaccc ccccttctgg gggacgtgag caatcccgag
    20761 gcggtccggg cctttggcgc cgacctcgtg ctcctcaaca tgttcgacgt ggacgccccc
    20821 cgcatcgaag gcctcccctc ggggggaagg tcggggtact ggaccgaccg gtacaacgcg
    20881 gcggtggccc cggctctcca ggggagcgat ccggtgcggg aggcgaagac cctcatcggg
    20941 cgccccgtgg gcatcaacct cgagcccctt cccgaagacc ccgccgtgct gggggtgcgg
    21001 gccgtgctgt cccccggacg taccgccacg gcctccagcg ccctgaaggc ccgggatctg
    21061 ggggtggact tcctggtgct cacgggaaat cccgccaccg gggtcacctg cggggccatc
    21121 accacggccc tccgagagct gcggcgcgcc ctgggggagg actaccccct catggcgggc
    21181 aagatgcatg ccgccgggag cgtggaagaa tcgggggaag gcatcgtcac ccccgccctg
    21241 gtggacgaat ggatcgacgc cggagccgac gtggtgcttc ttccggcccc gggcaccgtg
    21301 ccaggggtgg atgtcccctt tgttcaagag ctggtacgcc ggatccaccg ccggggggcc
    21361 ctggccctca cggcggtggg gacctcccag gagggatccg acgagggcac gatccgatct
    21421 ctggcgctgg cgtgcaagat ggccggagcc gatctccacc acctggggga ttcggggtac
    21481 agcggcatgg cgctccccga aaacatcatg gcctattccg tggccctccg ggggcgccgt
    21541 cacgcctatc ggcgcatggc gacctcccct cggcggtgag cggcgcggtg atcgacgacg
    21601 gacgaacgga gggaaagaga tgggactctt tgacgtgttc aagaaaaaaa gcgccttgga
    21661 gcttcgggcc cccctctccg ggagggtggt gtccctggac caggtgcccg acccggtgtt
    21721 tgcgggacgc atggtggggg atggggtggc catagatccc acggagagcg tggtctgtgc
    21781 cccctgtgat ggggtggtgg ccgccctttt ttccaccttt cacgccgtgg gccttcgcac
    21841 cgacgagggg ttggagatcc tgatccacgt gggcatcgac accgtggcca tgaagggcga
    21901 gggcttttcc agcgcggtga agatgggaga ccgggtcgtc cggggtcagg agttgttgcg
    21961 ctttgaccta gacctggtgc gagcccaggc tccttcgact tttagccccg tggtgctcac
    22021 ctccatgccc ctggtggagc gcttccaggc ggcggaaggg catgtggcgg cgggaaaaga
    22081 cgttctattt tccgtgatgt tgaagaccta gggcctccat cttggcccgg agcgcggggc
    22141 actttcttcg aagcagggcc gttggagctc cccgagaact ccaacggcgt tttttgttcc
    22201 cgcttcccta ggggaagtcg gaagggtccc ggggaagaat agttttaatt taataatgtt
    22261 ttacaaaaaa agtcaaaata ggcacaacgg gcgaaggctt gcgggaagcc gctcccttgc
    22321 tctaaaatct ccagtgaacc gttttggctc tcttcatacg gaattttatt tttcatggta
    22381 atccctcttc tgcttctata atttttttca taaatttatt cctgcggaat tccacaaaaa
    22441 gcttaggagc ccttcggcgg cgggggcaac cttgacaacc tacccaaata acatgtcgtg
    22501 gtgttttgct atgattgatg acacgaaggg gtccctcgaa ggggaagagc tggggacccg
    22561 gtcgccggag gggacgatcc cctcggattc cgcctcccgc ctgctaaact cgcccctccg
    22621 ggggtcttgg ccgggaattc ttgcgggggg tgagttccgg gtcggatctg gtgcgggaac
    22681 gtttgggccg gccgcacggc gcaaaggacc tccgctcctc ggggggatcg ggagcccggc
    22741 tcacgggccg aaaggaagga tgaccgtgcc tagagatatt tcttccgtcc gattgaaccg
    22801 cctcaacgac ttgttgcgct tttttcggga aaaggagtcc gtgccccggg ccgatctctt
    22861 tagccaattg ccctacaccc aacctcgcac gctggagcgg gatctggcct atctccggga
    22921 gcagtttcac gtgcagattt cctatgactt tcgtcgcaag gcctatgtgc tgaaaaaccc
    22981 cgggaccttt gtcctgagga tggccctgga ggagcgcgag gccttggccc tggcctcggg
    23041 gctcgagatg gtgcggcact ttttgccgca cctgggagaa ccctgtcatc aggtgtggga
    23101 gaagctccgc accatggtcc ccagccccct ggcccggagc gcggagcatc tcgctcgctc
    23161 ggcggtggtc tcccttcccg tgtcgaccat ggatccggga cactttgacg ccatcctgga
    23221 ggcgatgcgc cgtcgccgag tcctggaggc ccgctatgcc tcgccctaca gccaccccgt
    23281 cgaggagcgc tcctacgtcc tatcgccttg gggggtctac ttccgggccc atgcctggta
    23341 tctgtgggcc tggtcccacg gggcggagga ggagcggacc tttagagtct cccgtttcag
    23401 aaaggtggag ccgtcgtcga caccctatgt gcctcccccg gaggactccg gtgtggggga
    23461 tttcgccgac ggagcctggt atgcccaggg ggggcgagag ggaagggatg tggcggtgcg
    23521 catcgcccct cccctttccc tggtggtggc ggaaacccgg tggcacacca cccagagcct
    23581 ggaggcggcc gaagatggct cggtgacgct ccgggcgtgc gtcccctcct tggacgaggt
    23641 ggcccactgg atcttggcca gcgccccttt tgccgaagct cgagaacccc gagagctccg
    23701 ggagatggtg cgagggctgg ccgagacggc gggggccttg catcgagacc cggtggacgg
    23761 aaaggatgcg acaaagaccg acggagggga tgggccccgg agaaggggct tcccgccggg
    23821 tgacggaggg gcctccgggg aggccttcgg gccggaggag ggttccgggg cataaaaacc
    23881 ccccgcgaga cccgactttc gctttctttt gccgggaacg cccatctcgc tcgccggttt
    23941 ttctgcggaa tgggggctac gatgacgatc tttctcccgg aggagaaggg catctcgtgg
    24001 tacctaattt tttgtagaaa gctagtgatg gcaaaggatc agggcttttt tgcgcgaaag
    24061 tcgggaaaaa cctccgcgag aatcctcggg gagcctcctc gcaagggttt ttgttttagt
    24121 cctcggggcc gcctgggggg aggctggggg gctagagccg ggctcccccc cggcggctct
    24181 tgtacctccg gaggattacg tgggtccgca ctcggatgat gccgcgcttg gagtagatcc
    24241 ggtccagcac gaagcgctcc aggtgtccca tgtcttccaa cagggcgtgc atgtggaggg
    24301 tgctggctcc ggtcatgaga tagagatttt ccaccacgtc ttcctgggat aactcctcgg
    24361 ctaactggtc caacgagaag ggctccacgt cgatttcaaa aaaggcagag acgtttttcc
    24421 cgatcttttc ggggtttatc accacggtga agcgttctat gatcccttcg tctcggagct
    24481 tttccacccg agccttgatg cccacccggg tgaggccgta tttccttccc agctcggcat
    24541 agcttatgcg cccgtccgcc gcgaggtccg ccagcaggtt tcggtccagc gcgtccatgg
    24601 ccatgagatc ctctcctttc gtgacggtcc cgccgcccgc tcattttttt cccccaggtc
    24661 gttcccatcg ccggtccggc gacggggcgg tcccggccgt tttttcgggg aggtcctcac
    24721 ccggcgggaa acccccccgg gattagttcc ttccggagca gggcttccag ggcttccagg
    24781 gatctttttg ccgataggat cgccggtgac acggcggccg agtcccgcgt gacgcaggct
    24841 tcttccaggg cacgaaggcg ctccgttgcc cccccttcta cgaggtagga gatggatccc
    24901 cggagcctat gggccacctt ttccgcctcc tccagggtca tggggaggct cagctcctct
    24961 tgggcccgga tgacacaggc caggaattct tccatcatgc ccctaaagat ttcttggttt
    25021 ccgtggcaga tggccaggag ttgggttttt cgaagcgccg ccccttcgtc gggcgattcg
    25081 ggatctgcag ggggttgtcc gagagacacc ttcttttgag cctcctttcg aagggctagg
    25141 gccccccggg gggatttctc ccgggagcgg gtctttaggg ggccttgcgc cggaagagca
    25201 acgagggcct cccggggccg gaggggcacg aagacacttc caggaattcc cggtgcagga
    25261 gaaaatccag gtatcgggag gccgtggtac gggccagacc aaggcggtgg gccacttctt
    25321 gggcggtaaa ggtttcttcg tgccgccgga aaaagtccat gaaaagctcc acagtgggac
    25381 gctggagatt ttttggcaac aacatcatgg atgaagcgga ggatcggggg cgcagggcgt
    25441 caatggtgtc ctggtccggc acgtcgccca cctctcggac caagcggcgg tagtggcggt
    25501 aggcccgcag ggtctcctga aagcggccga aggtcacggg cttcaggagg taatcgaagg
    25561 cccccaggcg ccacgcctcg gcgatggcct ccgtggcgtg gtctgcggag acgcatatcg
    25621 cgtctatggc gtgaaagtcc ctccttaaaa gcctcagcag atccaatccg tgggcgtctc
    25681 ccaggcgaag atccaggaga agaaggtcca ccggccgctc ttggacgatg gaaattagtt
    25741 cctccccccc gtgggcccga cccaccaaga cgaacccttc cacactctca agaaagcgct
    25801 ggtgggtcag ggccgaaagt tcttcgtcat cggccaccag cgttcgaatg ggatccacgt
    25861 caccgccccc tcccaccgtt cggcctgagc gcctggaaga tcccctttat tcggcagggg
    25921 tttcgccgga aggaggagcc gccagcgccc gggccggagg tcatcttcgg cggccgcccc
    25981 gccatgggct ttttgaagga ccaacgatcg tcgggaaaga cgcgtcctcc accgacgaaa
    26041 ggagactgag acgacgacag agacccccca aaacactttt tttcaagtat actttccgtt
    26101 agaccatttg tctcgatatt ttgatgaaaa taaaggagat ggaaaaattt gttccatggc
    26161 gatcgagctc ggcagatctg gtggtcggac gtgggcctga tcggtatcgc cgtcttttgg
    26221 ggctccggct ttgccctcag cgacgtgttg atccagacca tatcgcccct gtggctcctg
    26281 gcggcccgat tttccctcag ctccctcctg atggggcccc ttttatggcg gcgcctccgg
    26341 gggcaagacc ttcgccttct gggggccgcc tccctggtgg gggtcctact ggggttgacc
    26401 tacgtggtgc atatctacgg cctggtgctc tccactccgg gaaaacaggc ctttatccag
    26461 agctccagcg tggccatggt ccctctgctt tacggcctgt ggtatggccg gtggccatcg
    26521 cggcccgcca ccctgggggc catcgtcacc acggtggggc tctttgtcct gggatttact
    26581 ccggggatgc tcttcaacgc cggggacgcc ctatcggtgc ttttggcctt cctggtggcc
    26641 ctgaacgtca tgatggtggg gtacctgagc cgccgcatgg atcccctggc gctggccctg
    26701 gctcaaattc tttcctgtac ggcgctgctc gtcgtcggag cttgggggtg tgaaccctgg
    26761 ccccggtggg gagagcttgg gacctggcag tggcaggccc tgggatttct ggcggtcttt
    26821 gtcaccgtga tccccttttt ggtgcagaac gtggcccaga agtacgcccc cgaggttcac
    26881 gccgccatcc tcctctccct ggagagcccc ttcggctacc tcatagcggt gttcctgggg
    26941 cgcgacgcgt ggcatatcca ggccgccctg ggaggagccc tcatcttggc gggggtgatg
    27001 gtggcggaat ccgaaagatg gctccccggg aggaaagatg ggacaaaaga gaagacctcc
    27061 gggggcgggg cgtgataaaa tgccaccatc ggggtgcccc ttctccgccg ggggcgagag
    27121 aagggcaatg gcgccgcgct ttttaaggga aatttccggg gggaagcccc gcccggggta
    27181 ggagggggtg agaccatgga ggagagcaaa atccgggaga attttgtcgc ggaggtccat
    27241 cgggcggaag aacgggtgga gcgccaaaag gaagagctcg cccgggccga cgacgaggtc
    27301 cggcgccggg cggcggagat cgtccaggca aaccgaaagc ccgtggtgga cctgggcatc
    27361 gacctggact cctgaccggc gcctcccgag gggcgtcctc acaccacctc gacccgcagg
    27421 gggccgatct gggccgtgtc ccccaccgcc aagagtcggg tgcctccggt gaggtggttt
    27481 tccaacacca catcgcctcc ccgcagggat atcgtggcag acaggggctt gagtccccct
    27541 tggggtcgca ggtagaccgt ggcgtcgggg gaagtgccga aggtcaccgg tgcggggcca
    27601 agcttcacca gggcccgctc ccggggggtg tggtggatca cgagccccgt gggccggggg
    27661 gggcgggggc gggtcccttc gcccccccgg gccccttttt cgccctcccg gaccccttcc
    27721 cggagggcgc gccgttcccg ggccaagtcg gcccgttccg ccacggtgag ggccaggccg
    27781 attcccgccc ccaggatggc ggcgcccacg atgcgggttc caccgctggg catgacgaac
    27841 gacgacagga cgaatcccac tccccccaaa agccccccgg caaagccccc cagggcgccg
    27901 tagaggcgtc gcaggttggg cacggcttcc gccaggagtc ttcccaggag gaggcccaaa
    27961 aggccccagc aaaagacccg ccacccctcc acgaaccact ggaaggactc tccgggggtg
    28021 gggggagata ccagaaggac gtaggccccc tgggccacca ctccggccag ggcccccgcg
    28081 cccagggccc aggggacgac gcgccgcagg tcccggccgg ataaaagggg ccttcgcagg
    28141 tagcggtgct gtccccccaa aaggcccagg gccacgcccc ctcccacgcc cgccgtccag
    28201 gccgccacgt agatcatccc gggcatgaac cccggcgccg gggagggaaa cgcctccagg
    28261 aggacgccgg ccatcccccc gcccccccct ccacacaaga aaaagagcaa agctttccaa
    28321 agatcgggcc tcatggtccc ttcctccctc cgggggtttc ttcgaggccc gtgaaggccc
    28381 gtggccttgg acaggccagc gcggcgccgg gccccggttt cggtcgatcg gcctgcgtca
    28441 tctcggctcg ggcctccggc caggggcttc cgggcccggg cggtgagggc gaaggattac
    28501 ccgacgggcc aggcctcctg gaacaaaagg gccacggtga catcgtcccc gctcccttgg
    28561 gtggacgcct cgtggagcca ggagggcagc gcctgctcca cggcgatagg tcccgcctcc
    28621 ccagggcgtt ccctcagcaa ggcgtaaaag tccatccccg cctgcaggaa atcttcgcga
    28681 cgggcaaagg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agcaaggcgt
    28801 aaaagtccat ccccgcctgc aggaaatctt cgcgacgggc aaaggagttg ccgtaaccat
    28861 ctgtggccaa aaggagcaga gagggccacc ctccggagcg ggggaggagg gccgtgcgaa
    28921 agtccagggg ggcgtcgggg aggcagaggg acgtggtgat gttggcgaag caccggggat
    28981 cggggggaag gggcgaaaag gttccatcgt cccggacggc gatgatgtcc ccgtccccca
    29041 ggtggaagat cagcaggtat tccgccgtcc ccagggctcc catcaccgtg gcgccatagg
    29101 ggcggaggtt cgtcgacccc gcagggtccg aggcatcccc cgggggatcg gcttcctggt
    29161 ccatctccac ccggtgccgc cagcgccaca ccagatctcg ggagaaatct tcttccacgg
    29221 cccgcttcag tggagcaaag ggaaggcctc ccgcccggcg ggccacgtcc tccagggcat
    29281 ccagggcctc ttccacggcg aaggcagccc cccgatcgct gcgaaagctc gccgggtccc
    29341 catggccgtc ggagagggcc accgccacgc cccggtccag gatccggagg cccagggcat
    29401 cctgattggg ccgtcccgtg cgccggtgcg aggccccttg gacgctagcc cccagggcgc
    29461 gccaggggcc gatcccgccg tcgtgccggt ttctcatgga gctccaccgg ggacttccgg
    29521 ggccccccgc acgatgccct ccacctctcc gaagttgatc cgcacctcgg gccccagcct
    29581 cacgctccgg tcggggggga cgtcgcgcag ggtgccgtcg gggaaggtgg ccacccaggg
    29641 ggtctccgag cagtttcgga ggccccagat ccgggggttt tggggatttt gccccacggc
    29701 gcccacgggg cgggagaagt cgaactccgg gtcgaggcgc acgtgccagg ggtacaggcg
    29761 ggtgtcggag ttcaggtaca ccgtcttgcg ccccagctcc agacgaaggg ggaggggcac
    29821 cggtcggcgg cagttccagc atgtcagggg gtgatcggga tggtcggggg cgttttccgc
    29881 gccgcaggag gcgcagtata ccaggctgtc ccggagggcc agggtggcgg cgcgccactc
    29941 gctttcccgt actcggccgt ggagggggtc tcccatccca tcggtgaagg acttctcgaa
    30001 aagcccgcga aaccacgagg ggtagagggg ccagaagacc agggcgttct ggtggtaccg
    30061 gggatcgggg cggttgcggt cgtctctggg gtcgaagatg aagagggggt cctggccgta
    30121 gagctggttc atggcgggaa ggtccaggca tcggatggcg gactcccgct gcccctccag
    30181 gggatgatgc atcatgaata gatagaacag aagcaccgcc aaggagaaga ggtcggtggc
    30241 cgtgctgggg gaggcctccc ctcggacgat ttccggagcc atgaatcgcg gcgtgcccag
    30301 gacgccggtg gtggtggtgc cgtttaccgc cacgttgtcg ttgtcgcaga tggccacatg
    30361 gccgctatcg gggtccaaaa agacgttgcc gaaggagatg tcccgatagc acagcccccg
    30421 ggcatgcagg ttcagaaagc tgtccgccag ctcgaagccc gccgtggcca gggctcgaaa
    30481 ggagggttcc gcccggcgct tcatgatgtc gttgatgccc ctcagggcgg ggtccctcag
    30541 gggcatgagg tagccaaagc cctccacccc tcggtggcgc accagatcca ggggccagag
    30601 aaagcgtcca tcgggggctc ctcgggccac caggagctcc agggctgccg cctgttcgcc
    30661 ggtggcctgg gcccggaagt accacttcag ggccacctct tgatcccccc aggagcttcg
    30721 gtacacctcc ccctggccgc ccccgccgag gaactcccgc acgatgcagg gcaggcccag
    30781 cgatgaccgg agttgttgtc ccgttttaag caacgatcct tccatgggaa ttttccccta
    30841 ccagacgtcg gtggcgtcct ggggcccgag gtccgagtcc ggcgggggga tgggcacgtt
    30901 tccccggggg gcgcttcccg gactctggct ggcgggggcc gaggcggagc gcaccaccgc
    30961 cgtggagacc cacttgatgt accgcaccag ggcctcgggg ttgttggcct ggaggggctt
    31021 gatctcgggg ttggcgatga agcgctggag cacgtccagg tcgcaatcgc cgccgatggc
    31081 gatggcgatg cgcacggctt tctttcccca ggggagggcc aggagttcct ggagcccctt
    31141 ttcgtaggcg tcggtgggct ggccgtccga cagcagcacc agcaccggag gaagggcccg
    31201 ttcgtccatg gggggcatgg tgagctgttc ggccaccatg gagagggcct tgcccaggtc
    31261 ggtttcgccg ccttcggcca gatcttgcca ccgaaaggcg tccacaggga cgggatccgc
    31321 cgacacccac cgggccccgc tggaaaaggt gatggtgcgc accagcaccg aggcgttggg
    31381 gttgttgtcc gcctccttct gcatgtgggg cagggcctcg cggatggcgg tgttgagggc
    31441 ctgcatcttg ccgtcggcgg ccatggaccc cgacgtgtcg gcgatccaga tgaagtgcag
    31501 gggacgggtg gccaactctc ctccgggacg tttcatggtc attcctcctc gagacgaaat
    31561 ttttactgga tgatacacct ttcgggggcc ctgctccgac gcccctcgtc cggggttccc
    31621 ctaggggcgg tttttgacgt cgatccagct ccgaaggcgc tgccataggc cccgaccctc
    31681 gccttcccct ccttcgaccc aggcccattt ttccgcattt ctccgggctc cctcgtgccc
    31741 ccgttccgcc gctcgccgaa accagtatag ggcccgggag aagtcctggg ggacccccac
    31801 gccgccgacg tacagggccc ccaagttgtt ctgggccacc gcgtgccccc gttccgccgc
    31861 ctgacggtac agatccaccg cccgggcgac gtcccggggc ttgccgtaaa aggcccgcag
    31921 ggccaagcga taggccgctt cggggtgccc ttgggcggcg gccgctcggt accatcgctc
    31981 cgcctcgcgc cgcaactctg gagccgcccc ctggcggtcc cagtgggccc ccagaaaaaa
    32041 ggcggcctcg ccatctccct gggcggcggc cctctcgagc cacaggatcc cctggcctct
    32101 ctccttgcca tcggagcccc cttccggcgg agaaaggtct tcttccagga gaaggattcc
    32161 cagacgcctc tgggcctccc tatccccccg ttgggcctcc cgggtgaccc atcgccgcgc
    32221 caggactcgg tcctcggcgt ctcggattcc ttcgccggct tccccgggga cgaaggggga
    32281 gaaatagcgc cgtcccaccc acgccaaggc ctcttcttgc cccgccgccg ccgcttggcg
    32341 taggagcgat cccccggcgc cggggtcttg tccctcgccg catcgccccg atagaatgag
    32401 ataacccatc agcgccaggg cctctgggtc tccaccctgg gcccggcgat ccgcttccct
    32461 tcgccttagg cgccgctttt cctggtactg gcgcaggagc cgccggtacc accggatggg
    32521 ggccgaaggc ggcgccaggg ctagctcctg ggcctccttt tcggggtcct cagggaaggg
    32581 ggccccgggg ccccggaggc gtcgggcgag gctacgggcc tccaggtccc cttccaatcg
    32641 atccaagagc tccagggccc gggatcggtc cggaggcccc tcccatccca gggcgaggcg
    32701 ctccccccag gccttgaggg ccttaggatc tcctaggtcg agccgacgcc gttgaaaatc
    32761 gcgagatctc cggaggaaga gctcttcttc cgtgagaagg ggcagatctt gagcttcccg
    32821 gtaggacgcc gcagcccggg agaagtctcg gggaactccg gccccggagg aatagagggc
    32881 tcccagggtg gatcgggcct cggcccatcc tgcctcggag gcctggatga tccaccgggc
    32941 cccttctcgg tcgtctcggg gcaccccgag ccccgctcgg tacatgagtc ccagggcgta
    33001 ggccgccgcc ccatcccctc cttcggcgct ccggcggtag gcctccgccg ccgaggcgaa
    33061 ggaccggctt ccgccgatcc cctgctccag gcagcgcccc agctctcccc aggcccccgg
    33121 atgcccctgt tcgccgctgg agcggagcag ggaccgcccc tcgacggccc ccgaaggggc
    33181 ttggcgccga attaggtccc atcccagccg gaaggcctcg tcggggtccg atgcgcttcg
    33241 cctccggcgt ccttcccgca cggcggcgat gtctccggga aggtaccccg tggcgtcctg
    33301 aagctccgcc agccagcgcc tcccctcctc gcttccctga agagccgcct cctccagcaa
    33361 gggggcggcc tcctccgggg acactccctt gtcgggggaa aagagggcct cccaggcccg
    33421 atccatggcc cgttcctccg gagggccctc gggggaaagg gggaagtgcc aggcttgggc
    33481 ccaggcctct tggtccagct cccgggcccg atcctccagg gatagggaag ggcttaagat
    33541 cgtctcgggg ggcggggcct cgtgggaggc tttgggactt cgccgggggc cttccagagc
    33601 agccgtccag agctctaccc cccgggcggc ggtggcggcg tccaaccccc gctcctcccg
    33661 aaggtgacga agcgccaggg agcgacgcgc ctccggagcc agggcctcgg agctcaggag
    33721 ggcgggaagc ctttcctccg ccgccacgac gaccaaggtc acgtcccggc gctgggcggg
    33781 gcaccagtcg cgcagcaaat tcctcaggcg c
//
LOCUS       DAOOER010000017        32791 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_1520_length_32791_cov_9.623564, whole genome shotgun sequence.
ACCESSION   DAOOER010000017 DAOOER010000000
VERSION     DAOOER010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 32791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32791
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1520_length_32791_cov_9.623564"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PL162_03875"
     CDS             <1..250
                     /locus_tag="PL162_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91197.1"
                     /translation="DAMGRIAVSEARRRLFQLVGEAAEWHVPASIAGRRNTAALVEES
                     DWRVVEEALFLFSIPGVRESIVDGTATPIEDCSENAGW"
     gene            1435..1848
                     /locus_tag="PL162_03880"
     CDS             1435..1848
                     /locus_tag="PL162_03880"
                     /inference="COORDINATES: protein motif:HMM:NF020360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HQF91198.1"
                     /translation="MTNNTLDIGPLERAVQRLQEGLVRHRQDVDDAQIRDGLIQRFEF
                     TYEISHKMLKRYLESISADPQQYDGMPFQDLIRSGNEHGLLRGDWEDWKRYRNMRAKT
                     SHTYDEHSAHEVVDEIPRFLEEVRSLCDRLRSCLP"
     gene            1845..2204
                     /locus_tag="PL162_03885"
     CDS             1845..2204
                     /locus_tag="PL162_03885"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HQF91199.1"
                     /translation="MTRETPHIDIRPDHWIMVRDILRTHVPQYDVWAFGSRATGTAKP
                     YSDLDLAIITDHPLSLAVSAALADDFSESDLPYKVDIVDWAATGESFQRIIERHKVVV
                     QEGKRNCGMDSERRSST"
     gene            2721..4100
                     /gene="tilS"
                     /locus_tag="PL162_03890"
     CDS             2721..4100
                     /gene="tilS"
                     /locus_tag="PL162_03890"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HQF91200.1"
                     /translation="MESSLFLFPHLSEDHRRRFIAAGTRQGWFHRPEPLVVALSGGGD
                     SMALLLLLLAVTPASRLVLAHAEHGLRGEASREDARFVQAVGRQFHLDTEVAFLAVRE
                     NRCPGESIEVCARRLRLHFLEETARSRGALWIATGHQADDLAETVLFHQVRGAGGRGS
                     AGFREVRLPYVRPVVDFTREELRSLLRAQGFSWREDGSNDSPEYTRNRIRHTVLPLLE
                     SAVHPGATRHLAALGREMAEREDREDGEAGALLAWVRRPFPRAERAWRGDVAVGLKDH
                     LLARLLRLEARSLGWGALDRRRTEDLLSLVRRPSSCWRFQWQEDRELVGGRPLWGWVR
                     RPWGEPLGAVPLPPEGEWRWGPWRCRWEDTVAPEGRSFGGWGLGLRFCAPPEKSFLVP
                     LASLDGETRRKAPVPRWCHAWWPAVEGPEGARILPWDRGDLSLPPSGGCDRMDRLLSF
                     SLGKEFACS"
     gene            4097..4630
                     /gene="hpt"
                     /locus_tag="PL162_03895"
     CDS             4097..4630
                     /gene="hpt"
                     /locus_tag="PL162_03895"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HQF91201.1"
                     /translation="MSYELGHPILEEATIRHRVRELGEKISQDYDGKTVVCVGVLKGS
                     VVFLSDLVRAVDGRRVDVRLDFMAVSSYGNSTESMGVVKILKDLDTDIQGCHVLLVED
                     IVDSGLTLSYLKRLLGERNPASLRVCVLLDKPERRRVEVSVDYRGFVIPDEFVVGYGL
                     DYGGRWRGLPAVYRVTV"
     gene            4759..6681
                     /gene="ftsH"
                     /locus_tag="PL162_03900"
     CDS             4759..6681
                     /gene="ftsH"
                     /locus_tag="PL162_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HQF91202.1"
                     /translation="MNRLVKNLGVYLVLVVLVVSVVNVFLSPDKASQEAQRLNYSKFL
                     KSVDDGLVQKVVIDEDGIQGTLVNGTPFVTYTVGVGDLAPKLVGKGVDVAVAPPQKTP
                     WWTGLVSTLFPTLLLIGAWFFIIYHMQGGGNKVMNFAKSKAKLFLDNRPKVTFSDVAG
                     CDEAKEELQEIIDYLKDPARFTTFGAKVPKGVLLLGSPGTGKTLLSRAVAGEADVPFF
                     SISGSDFVEMFVGVGAARVRDLFEQARRYQPCIIFIDEIDAVGRQRGAGLGGGHDERE
                     QTLNQLLVEMDGFDTSTGIILIAATNRPDILDPALLRPGRFDRQIVVDRPDVKGRLAI
                     LEVHAKDKKMGTDVDLNVIARRTPGFVGADLANLVNEASLLAARRGKGAVEMAEMEEA
                     IDRVIAGPERKSRVINAKEKEIIAYHEAGHALAAKLIPGCDPVHKISIIPRGHKALGY
                     TLQIPEEDRYLVSKKELSTRIQSLLGGRVAEEIIFKDVTTGAQNDLERATLIARQMVT
                     QFGMSERLGPVTLGRKQHEVFLGRDIVDDRNYSDEVAFAIDQEVRRIIDEAYEGVRAL
                     FVDHRDLLTKIAETLLDREIIEAEELNVLMGNGAEEPAPAEPSPEPAKEEPTAEPFRY
                     PRPTPRAPGEVSIKPA"
     gene            6794..8794
                     /gene="uvrB"
                     /locus_tag="PL162_03905"
     CDS             6794..8794
                     /gene="uvrB"
                     /locus_tag="PL162_03905"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HQF91203.1"
                     /translation="MEEPDRFLLESPWPPSGDQPQAIEALARGVELGHRFQTLLGVTG
                     SGKTFTIAALAARLHRPVLVLAHNKTLAAQLYSEFRGFFPHNAVHYFVSYYDYYQPEA
                     YVPAQDLYIEKDASVNDRIEKLRLATTKALLERRDVVVVASVSCIYGLGQRKSYEDAV
                     FSFAVGDRWERRAFLERLMQNYYERNDVAFSPGLFRVRGDVLEIYPAYSDTALRVAFF
                     DDEVERIDEIEPVSGQRVLSKMKASIFPAKHYVTGPDAIKNAMEHILADMENQAAKFQ
                     REGKLLEAQRLVTRTRYDMEMLTEVGYCSGIENYSVYLDGRSPGDPPGTLLDFFPKDF
                     LMVIDESHISVPQVRGMYNGDRSRKEVLVDYGFRLPSAMDNRPLRWEEFEEYMHQVIF
                     VSATPGDWEIAQSSQVVEQIVRPTGVVDPEILVLPARSQVDDLVGRLKDVVERGERAL
                     VTALTKRSSEDLAEYLGDLGFKSKYIHSELDAFERAELIRDLRLGRIDVLVGVNLLRE
                     GIDLPEVSLVAILDADREGFLRSERSLVQIIGRAARNVAGVVILYADQDTGSIQRAVA
                     ETDRRRKIQQEYNEKHHITPQSVRKGVGHLLPEELLEKGTEGSEESDEIMPRETLERM
                     MWEAVERLDFERAAQLRDQLAGKKKGATLRVASPRRARRARA"
     gene            8754..11585
                     /gene="uvrA"
                     /locus_tag="PL162_03910"
     CDS             8754..11585
                     /gene="uvrA"
                     /locus_tag="PL162_03910"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HQF91204.1"
                     /translation="MSQVLVVRGAREHNLKNAHVTIPKDRLVVFTGPSGSGKSSLAFD
                     TIYAEGQRRYIESLSSYARQFLGVQGRPDVEDISGLSPAISIEQKGRSHNPRSTVGTV
                     TEVYDYLRLLFGRIGVPACPSCGKRLSRYTLDEIVDLILRDYDGKRIEILSPVLRGKK
                     GEHRNAFQQIRQKGFLRVRVDGTVFWLEEEIPLDKNKKHTVEIVVDRLSAREDRRTRM
                     SEAVESALRESKGFVLLLPEGEKERLLAENFVCPECDVAFPEISHRLFSFNNPAGACP
                     DCTGIGSHEHFSEELAVDPDLTIAQGALLPWEGTHYMLRRLQALAEAKGWDLTLPFRE
                     LSPGLRESILEGDPDRVPMTYKEHGEEHVYQGRFEGLLPWLTRRWEETESENVKEDLL
                     RFRMEKVCPSCGGLRLRPEALSVFVGGKNLGELVSLPLDALGAFLRDLTLNSWEQDVV
                     RQVMDELNKRLSFLLDVGVEYLSLSRRADTLSGGESQRIRLATQIGSKLTGVLYVLDE
                     PTIGLHPRDTARLLRTLGTIRDLGNTVLVVEHDRDTMKAADFLVELGPAAGERGGEIV
                     AVGTPEEVMCLSTFTGPYLKGETCGVFRPYAERRRPREERLVVRGAREHNLKDLDVSI
                     PLGVFSCLTGVSGSGKSTLMHEVIYKGLRRRMDRSFRGRAGDHRDIEGWQKLRHVALV
                     DQSPIGRTPRSNPATYTGVFTAIRELFAQLPEAKIRGYAPGRFSFNVRGGRCEACGGD
                     GVVKVSMLFLPDVYVTCDVCRGARYNRETLEVRYKGRHIAEILDLSVEEAVTVFQDIP
                     RIAGKLRVIEEAGLGYIRLGQSATTLSGGEAQRVKLSTELSKRFSGTTLYLLDEPTTG
                     LHYTDVKKLLVLLHKLVDQGNSVLLIEHNLDVLASADYIIDLGPEGGAGGGTLVTEGT
                     PEEVARSPYGHTGRCLRAYLQEIREGGMA"
     gene            11582..12379
                     /gene="rlmB"
                     /locus_tag="PL162_03915"
     CDS             11582..12379
                     /gene="rlmB"
                     /locus_tag="PL162_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HQF91205.1"
                     /translation="MSRPSRRPAPPPEIPERGRGLEDEGWCWGKHPVMALLRESPARV
                     LKVLLLSRVGGWDPVLGLCNAHRIPVIWADGPALDRHCDGGAHQGIVAQVAPGPLYAL
                     EELPSLLPEEGLPALVVLLDHLQDPHNMGAVIRTAEAAGAKAVVFPKRRGVLPGGTVA
                     KASAGAALRLPLVAVTNLSRAIQELQRWGFWSVGLEAAAPEGLWSPSPLPRRLALVVG
                     SEGEGLARLVAATCDELRSIPMRGEAGSLNASVAAALGMFEWVRQWG"
     gene            12508..12582
                     /locus_tag="PL162_03920"
     tRNA            12508..12582
                     /locus_tag="PL162_03920"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12540..12542,aa:Val,seq:gac)
     gene            12960..13037
                     /locus_tag="PL162_03925"
     tRNA            12960..13037
                     /locus_tag="PL162_03925"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12995..12997,aa:Pro,seq:tgg)
     gene            13058..13134
                     /locus_tag="PL162_03930"
     tRNA            13058..13134
                     /locus_tag="PL162_03930"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13092..13094,aa:Gly,seq:tcc)
     gene            13150..13226
                     /locus_tag="PL162_03935"
     tRNA            13150..13226
                     /locus_tag="PL162_03935"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13184..13186,aa:Arg,seq:tct)
     gene            13265..13341
                     /locus_tag="PL162_03940"
     tRNA            13265..13341
                     /locus_tag="PL162_03940"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13299..13301,aa:His,seq:gtg)
     gene            13352..13427
                     /locus_tag="PL162_03945"
     tRNA            13352..13427
                     /locus_tag="PL162_03945"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13385..13387,aa:Arg,seq:tcg)
     gene            13442..13517
                     /locus_tag="PL162_03950"
     tRNA            13442..13517
                     /locus_tag="PL162_03950"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13475..13477,aa:Lys,seq:ctt)
     gene            13563..13638
                     /locus_tag="PL162_03955"
     tRNA            13563..13638
                     /locus_tag="PL162_03955"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13596..13598,aa:Lys,seq:ctt)
     gene            13657..13739
                     /locus_tag="PL162_03960"
     tRNA            13657..13739
                     /locus_tag="PL162_03960"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13691..13693,aa:Leu,seq:tag)
     gene            13953..15815
                     /locus_tag="PL162_03965"
     CDS             13953..15815
                     /locus_tag="PL162_03965"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HQF91206.1"
                     /translation="MSVQDQSCPFLLEGQLPARWRREVDGALRAACWAGALLREEADR
                     PGGPRGSGGHAEIDEEVEWGIHDFIAENFPEDGWLGEETGVRIRPGTSGRVWVVDPND
                     GTREFLQGFRGPAVSVALVAEGIPVLGVVYAYNHPDDRGDLIVGVVEGGFVFRNGVRV
                     ERLWREIPSDEALVIVAPGGEKNPVANLQCAHPLRFRVMPSIAYRLALAAVDEGDVGF
                     ALMGPVSWDVAGGHAILLAAGGDLYGVSGTPLRYDRRGEFPGQSLLLGGPRGLVEHLR
                     SKDWSRVTYPKSDGSPSALCRPLRSREALDPHVLARAQGCLLGQLAGDALGSLVEFES
                     PEDIRRLHPGGVRDLVGGGPWGTIAGQPTDDSEMALALARTLVADGSFRVDHVAQAYR
                     AWLASDPFDVGHTTRQALLGEPQPDSQANGALMRISPLGIFGAFCSPEDVAFWAAQDA
                     VLTHIHPICSQASALFARAVAHAIRNGGSGEGLFDHMARWAEEGELDPAIRQLFPRIA
                     RRERPVETVHAGWVLVALQNALWHLRHSSSLEEALEDTVMRGGDADTNAAICGALLGA
                     VFGRDGVPQRWQKRLFSCRPAAGVRGVLHPRPDCYWSVDALMLAERLLRAGMGR"
     gene            16000..16497
                     /locus_tag="PL162_03970"
     CDS             16000..16497
                     /locus_tag="PL162_03970"
                     /inference="COORDINATES: protein motif:HMM:NF016999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HQF91207.1"
                     /translation="MNVQQGLVALAVLGGGVGGNALGVPAGYLVGGFVGGLLAKGATG
                     LDLLPGGEAVTVVGQVLVAYAMVCSADLSAWRAFPRLLPVAVLSAAVLTAASLVLAKV
                     LEKVCHLDPMTALFSTPPGGLTGLAVAGASMGSDSPISLLFQMVRLIFVLLVIPFVAS
                     WWTRW"
     gene            16545..17396
                     /locus_tag="PL162_03975"
     CDS             16545..17396
                     /locus_tag="PL162_03975"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQF91208.1"
                     /translation="MLWGALTFMLFTWSSAFPAIRIGLSGFSPTHLALFRFLVASGAL
                     ILYALAVRMPLPEMRDVPKLAGLGCLAITLYHIPLNLGEMTISAGAASLLMSTAPVCT
                     ALLAAAILGNPFSRGGWIGLGVSFLGAGLIALSEGGLGGFSVGALWVLLSVVSESLAF
                     VLQGGMLKKYGPLKTGTYLMWTGTAFMLPFGGGLFDAIAKAPWEATAVTIYLGLFPAA
                     LAYLCWSLVLVRLKPAHATATFYAIGPLALLISWLWIGERPLPLAIGGGALALLGVWI
                     VERSRRT"
     gene            18178..19974
                     /locus_tag="PL162_03980"
     CDS             18178..19974
                     /locus_tag="PL162_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HQF91209.1"
                     /translation="MGKFGDILRIDLAKKSVTREDVDENQLRDYIGGRGYATKMVYDE
                     VPKGTDPLSPQNKLIFACGPLTGTSSPASGRYMVVTKSPLTGYIASSNSGGFWGAELA
                     KAGIFALVLEGKADAPTYLWIKDGKVELRDASKVWGKDGHETTDMLLAEVGDDKARVA
                     CIGPAGEKLSLLAAIMNDKDRAAGRSGVGAVMGSKNLKAVVVRGTGMPYMHDVNALRD
                     ALRDGVHKLKENGVTGKGLPAYGTAVLVNIINQTGGYPYRNWQDAYMEKADSQSGETL
                     ADKYLKKNSACFGCPIGCGRVTEVDGKKGEGPEYESIWAFGSCCGVEDLEPILRANVV
                     CNELGIDTIGAGVTVAAAMELYEKGFIPDGDLGGGPKPLFGDGDAMVHWVTMMGKGET
                     PLGKRLALGSYRLCEAYGHPEFSMSVKKQEMPAYDGRAIQGIGLTYAVSNRGGCHVRG
                     YLISPEVLGIPDKLDPEDIGAKPQWAKIFHDLSAAIDSAGLCLFTSFALGAEDYAGYL
                     RAATGFDYTADEVMTCGDRIWTLERLFNLREGLTAAEDTLPSRLREEPIPSGPMKGKT
                     SRVAEMVPEYYRLRGWDEKGVPTPEKLRSLGI"
     gene            20086..20355
                     /locus_tag="PL162_03985"
     CDS             20086..20355
                     /locus_tag="PL162_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HQF91210.1"
                     /translation="MKVLFFGFVRDMTGEKETAVEGAATIISLFEALATRYGERFRTE
                     ALPEGELSSELIVLVNGRHIAHSGGAATPLREEDTVAIFPLVGGG"
     gene            20731..22125
                     /locus_tag="PL162_03990"
     CDS             20731..22125
                     /locus_tag="PL162_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HQF91211.1"
                     /translation="MKLLGLLAPLGVFGAYTLAVILLTGPGFVWNENRRRFYLAGRGA
                     GLWASVATFGATWMSAASLLGYPESFFWDGMVAFGGAVNGWLLGLLPLPWIVAVLRRS
                     RALSLPEWIGKTFDDPRLRRLTAAALLLAYVLYILIQFRSFGGAVSHLLGIRYAVGAS
                     ALVYLFVLYTTFGGMPSVIRSDVLNFWLIVVGVTVAAVVAVGRVGGVEVLVRRLATEA
                     PELLNPAAPWEVLGGMAAMTLGWGLGVAANPQYAVRILSARSPRVALGMLAIVPFFLG
                     VIYLGLTAFAATGRLVAAPVLRQGGEAAFLHMVSMVLPPAPASLFLVALIAAAVSTAN
                     SQLLLAASSFCYDLMDRWRTTLPEDIFLFRNRLAVTVIATLALGGSLLPFPDILTVGR
                     FSWTVVALCFFLPLFWPLSGRRQGLFEAMGLSLVLHGVLVFGLHLPPEGSLLPSLLLE
                     RLSWRWFSRKGEEA"
     gene            22127..23407
                     /locus_tag="PL162_03995"
     CDS             22127..23407
                     /locus_tag="PL162_03995"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQF91212.1"
                     /translation="MGRPRHMESQIKALVVAGVLLLTAVTLAVSIGQGFALRWEENRR
                     WLYTVRSVLEALDRPQAEIVVAMLRPAWRVFPGTAGRSGGEILRSRDGEMWAAFPNDE
                     VLWEELLESNRPLLVVALVTLLLVLEISIFWAYAIVRPLRRLAWACRDMGSGRWPDLG
                     PEGASGTYEIATLHEAFRHMVDRLQQWQRVERQVGRMERLAALGQVVAGVAHEVRNPL
                     ASLRIHLDLLEVDVLPAGQPSLRVLGDEVDRLNQIVSQLLAFSRPRPPIGGPVDLGEV
                     VSWCHQMVRDRMSRQGVSWRETGFDGARPVPGDAAQLRQMLLNLVLNALDAMPRGGEL
                     HVGTRYGEDDGPGGVRRAALFLSVEDSGEGIASELLPRLFDPFFTTRPEGTGLGLALV
                     HRIAELHGGAVDVVSSPGRTIFTVTLPEREGDAP"
     gene            23404..24747
                     /locus_tag="PL162_04000"
     CDS             23404..24747
                     /locus_tag="PL162_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQF91213.1"
                     /translation="MMRMWIVEDEAGLSQGLARAFERDGYAVRVLDRLGEVRGALAQE
                     RPDVVLLDIRLPDGDGLAALPHLLREAPEARVVVMTAFGDSSLVVRAIREGAWNYLDK
                     PFPLEAARNMVSRAVESLELSRRVARLRGESLPLVGSSAAMERVRAFVAKVAPFRDLN
                     VLLIGESGVGKEVVARMLHAASGARGDFVALNCAAVPEALLESELFGYRRGAYTGAGQ
                     DKEGLAETAAQGTLFLDEIGDMPLVLQGKMLRFLETRTLRPLGSTKEIRLSLQVMAAT
                     CVDLERRVADGTFRRDLYFRIAMLPLVIPPLRDREGDVRELLESFLKEFALKLNRPCL
                     QVTPEVEEALRHYAWPGNVRELRNLVERLYILRDREDGLVRLGDLPEEMLEPLPQECL
                     EGIREGGLVQRLDDYERRLLREALEEAGGNRTRAASALGISRFALLRRLQRHALE"
     gene            24734..25948
                     /locus_tag="PL162_04005"
     CDS             24734..25948
                     /locus_tag="PL162_04005"
                     /inference="COORDINATES: protein motif:HMM:NF016827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91214.1"
                     /translation="MPSNRPSLLQGPRLTTALLPLPLLLPRIALASSPLEGLTRDLED
                     LLASNPLVRVIPPGGEMGDSSESEENDEERIPEDLLSLDDHLRRQLAEDPLDGFDFDS
                     SQLGETLDPRGYLNVPLVPWGVHLGLSPEEAPRALAALQEWADPPGLFARDLTECLLI
                     QLRRKLGEEGDGGRILREGREDLVRGDQHTLARRLEWPSERLQRALKELQALDPHPGY
                     AFRKTVMVRPELFFRPLGPGKELSVHLAEENLPRLALEAKGRSPGESGYDHAAWHEAR
                     AVLGALAQRYRGRVEVGRFLAKRQRAYLMGDLPAPEPLVLADLHREMGYHPSTLSRVL
                     RSTWAVTPRGTVLLGHLLSRPLQGRPDLSVAALRHLLRQARHEGRSWAEVARELALSP
                     RTVAWHGLRCGG"
     gene            26152..27387
                     /locus_tag="PL162_04010"
     CDS             26152..27387
                     /locus_tag="PL162_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HQF91215.1"
                     /translation="MAEEKGTAGAPLGAAEDRKGTLAILLGAAFLMATSAIGPGFLTQ
                     TAVFTEKLKGAFAFAILASILIDVVVQLNIWRILAVSGLRAQDLANKVLPGLGYFLAF
                     AVSLGGLVFNIGNVAGAAMGLNVLAGVPLIPGALLSAGLAVVLFLSKEMGRAMDRFAQ
                     VLGFVMIAMVCYMVFRTNPPVGTALKEMVLPSTVDWMIVLTLVGGTVGGYISFAGAHR
                     IIDAGITGPRALGDISRGAVNAIGITGLMRFLLFLAILGVVVMGTPLDAANPPASAFR
                     IGAGELGYRIFGVILWAAAITSVVGASYTSVSFLRTLAAPIESYHRWWIIGFIAVSTA
                     IFVTVGRPVTLLIFAGAINGLILPLALISVLLGAHRKNIVGDYKHPMWMSVAGYVMAA
                     FTLWMGFTSLGPIFTMFQK"
     gene            27459..28205
                     /gene="pxpB"
                     /locus_tag="PL162_04015"
     CDS             27459..28205
                     /gene="pxpB"
                     /locus_tag="PL162_04015"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HQF91216.1"
                     /translation="MDGPTKPRVLFAGDRCVVVEFGTAIDRAVNARVQQLRRDLEARP
                     LGGITELVPTYRSLAVYFDPRRVGEGLPELLASRAASLEGSAETGGRCVVIPVGYGGE
                     RGPDLPEVARHAGLSESEVVSRHTGRDYYCYMLGFTPGFAYLGGMDETLATPRLANPR
                     TKIPAGSVGIAGAQTGIYPIDSPGGWRLIGRTPLRLFDPDRTPPTLLDAGLWVRFVAV
                     DEGEYAAVTDQVRRGAYVPEVRDREGNHEA"
     gene            28195..29253
                     /locus_tag="PL162_04020"
     CDS             28195..29253
                     /locus_tag="PL162_04020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HQF91217.1"
                     /translation="MRLEVRRPGLLSTVQDGGRWGYQGKGVPVAGAMDLPAFRRGNLV
                     AGNEPEEAALEITVLGPTLGVLEGEGIVAAAGADLGFALNGAPAPLLTAVRVRSGDEI
                     SFAGPRGGGCRGYLCFAGGVAVPPVMGSRSTFLRGALGGFHGRALRAGDVLETGDLPP
                     LAFAGEGFSCPPDLDLDRSADRPLRVVPGPQDDAFTAEGREIFFSSAYRVTDEADRMG
                     YRLEGPPVAHSRGADIVSDAVPLGAIQIPGHGRPICMLADRQTTGGYPKIAVICSDDV
                     GVLAQRMAGDTVRFVAVTFAEALRWAKEEGDALENLRRARSTWRSRPRFPAGWDASGR
                     WTLTVGERAYAVTWRARS"
     gene            29280..30044
                     /locus_tag="PL162_04025"
     CDS             29280..30044
                     /locus_tag="PL162_04025"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HQF91218.1"
                     /translation="MMMMDLNSDLGESFGAWTMGDDEAVLASVSSANVACGYHAGDPE
                     VMLKTVRAAARRSVGVGAHPGYPDLVGFGRRNMACSPDEVYAFTLYQIGALTAVCRAS
                     GVSLRHVKPHGALYNQAAKDPRLAEAIARAVKDGGEGLMLLGLANSAFGGAAATVGVP
                     FAAEAFADRAYRADGTLVPRGEPGAVIHDESVAAARVVRMATEGVVEAIDGTLVSLRP
                     HSVCLHGDTKEAVTMAREVRRALEGAGVEIRALEVR"
     gene            30044..30844
                     /locus_tag="PL162_04030"
     CDS             30044..30844
                     /locus_tag="PL162_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HQF91219.1"
                     /translation="MKSFSDMQGLSPREIREIIRRGEWDAPTPGLAPGHVQANLVILP
                     REWAYDFLVFAMRNPKPCPVLDVTEPGDPEPRKMAPGGDLRTDLPRYRVWEQGQCVAE
                     PREIGEYWRDDLVGFLLGCSFSFEGALTEARVPVRHIECGCNVPMYITNVPCAPAGRL
                     SGPMVVSMRPIPASLVPRAVLATGRFPAVHGAPVHVGDPGAIGIGDVQRPDFGDPVPI
                     REGEVPVFWACGVTPQAALMASRPPFAITHAPGHMFLCDPRDSDLAVF"
     gene            complement(30899..31465)
                     /locus_tag="PL162_04035"
     CDS             complement(30899..31465)
                     /locus_tag="PL162_04035"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQF91220.1"
                     /translation="MTSLREFLLCHVLCPCYTSFHPGGHGPCPTEEGALGLLFPDPET
                     PYSALVDVGCGSGRVLAWWLQHGFADKNLTGIEVNRGIAAWTRRRFRSSPQVRVLSGD
                     AVRLVSNLPPVPTLFYLFNPFDASTLGGFLREVRLRYAPHGAEIVYYNSEHASAFLED
                     PAWTVTPLALPDPHWPALRARLGPIPPS"
     gene            complement(31663..>32791)
                     /locus_tag="PL162_04040"
     CDS             complement(31663..>32791)
                     /locus_tag="PL162_04040"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HQF91221.1"
                     /translation="YLRVNELFASAVARHTTPRNLIWIHDYHLIHVAEMLSRQDVSRR
                     CVFFLHIPFPPLDIFAKLPWRMSLLRALTAYDLVAFQTARDRRNFLTCLRAFFPSATV
                     HGRGPVMTSTVEGRSFSAAAIPISIDYDDFDRTARSAPVKRQAALLREQYRMEKLALG
                     VDRLDYTKGILERLMAFQSALERHPELRGRLSLVQVVVPSRETVKAYRDLKERIELLV
                     SGINGKYTKDGWIPVVYLYQSMDRSQLVAHYLAADMALVTPLKDGMNLVCKEYCASHV
                     DRRGVLILSEFAGAAAQLSDTILINPYDTEGTAEAIWKAFEMSEKEQRRRMVSLRRRI
                     QRQDVFWWVGTFLQAAAGVELDDFPEVELAPILPLRRHEGS"
BASE COUNT         5532 a         9201 c        11590 g         6468 t
ORIGIN      
        1 tgatgccatg ggccgtatcg ccgtatccga ggcccgcagg cgactctttc agcttgttgg
       61 cgaggcggcc gagtggcatg ttcccgcgag catcgcgggg aggcgcaata cagcggcgct
      121 ggtggaggag tcggactgga gggtcgtcga ggaggcgcta tttctgttca gcatccctgg
      181 ggtgcgcgaa tcgatcgtag acggtacggc gacgccgatc gaggattgct ctgagaatgc
      241 cggatggtag ctccgtggag agtcgtcttc atcagaggga tccaggcgtg gcgtgcggct
      301 atctgcagat cgacggtatc ggcagggata acgtcattcg cctccccggc agacgaatga
      361 ggccatggtg ctcgtaaaag gcaacggccg ggtcgtcctt ggcatcgacc acaagggcat
      421 acgcggccat ctcggatcgc cttacgcgca aaagcgcatc ggccagcaga gctccgccaa
      481 acccgcgtcc tgcgctccta acgtcaaccg tgaggtcgac gcccggattg ccctggaacg
      541 ttcctttggg ggccatcccc ccgtgtgggg gatccccgta tcgaagagcg gcgccggaag
      601 aaacgttctg gcaaagggca tctcgcctcg ggaaagaaat agatacgtgg cagcgcaccc
      661 agggaggtct tgtgctccgc tcggacgcag cggggggtgc gacatgtggg tttgacaatc
      721 ggccccagca taaatatatt agaagacgtt ttgcgctggg gaagcggcgc tcgcatcccc
      781 atgaccgcct tgagcgcgac aagaaaaccc atcgcccatg aagaattcga gaaatggccg
      841 aggtggaaga ccgctggtca tcaggagacg agatagacaa gtccctcgat gtcggcggtg
      901 acgccgtgtg ccactggatc gacaagcatg cgatgcccgc caatcgtagg ggttgccttt
      961 ggaagtttca gaaagattaa gtcgacgagg gggagagggc tggcggtgcg acggacaaga
     1021 gcagaaagga tccagcggga tgagcaggag ggacggaaag aacaattccg tcggtctgga
     1081 tatcgggacc cttttgccca ggcggtcggc taggacgaga tccgagagca agtcggcgac
     1141 ctcagcattc cgctatactt gcggggggat cccggcggtg gccgtggcga cggagtgacc
     1201 gaaaggatct gctggagcgt gcttgtggca aaataggaac tcccggagcc gcaagacttg
     1261 acgccgccat cgacgtcaat ttgagggagc tggggctatg accgcaggaa ccccacgcca
     1321 aacgccgcaa aaggcactcc atggcgcacg ccacccggcc cacggcggag ccgtgccgta
     1381 ccggtgcacc agatggaaag cggtatgagg ccgagtgttt gaggaggagc ttccatgacg
     1441 aacaacacac tggatatagg ccccctagag cgggcggtgc agcggctaca agaaggactg
     1501 gtgcgccaca ggcaagacgt cgacgacgca caaatacgcg atggtcttat tcagcgcttt
     1561 gagttcacct atgaaatcag ccataagatg ctcaagcgct atcttgaatc catctcggcc
     1621 gatccccagc agtacgacgg catgcccttc caggacttga tccggtccgg caacgaacac
     1681 ggactcctgc ggggggactg ggaggattgg aaacgctacc gtaatatgcg ggccaaaacc
     1741 agccacacat acgacgagca ctcggcgcac gaagtggtgg acgaaattcc ccgctttctg
     1801 gaggaagtcc gctctctgtg cgaccgactc cggagctgtt tgccatgacg agggaaacgc
     1861 cccacatcga catccgcccc gatcactgga tcatggtgcg cgacatcctg cgtacgcatg
     1921 tgccacaata cgatgtttgg gctttcggct cgcgcgccac gggtaccgcc aaaccttatt
     1981 ccgatctgga tctagccatc atcaccgacc accccttgtc ccttgcggtg agcgccgcgt
     2041 tggcggacga cttttccgaa tcggatcttc cttacaaggt cgacatcgtc gattgggcgg
     2101 ccaccggcga gtcgtttcaa cggatcatcg agcgccacaa ggtcgtggtg caggagggaa
     2161 aaaggaattg tggtatggat agtgagcggc gctcgtcaac gtgatgggga gaaatcgcct
     2221 tagaatatag agccgtatgg caaaataaca actaaaataa ggatatttat taattgcttg
     2281 agtcggacga cccaatagtt taggcagtgg aagcgctggc cttcggctct ggggtgcctc
     2341 cgtcctctct gtctggccgg gaacggctta acatcggtgg cccttcaggg tgggggaggc
     2401 agaaaaagag gatctttcta tccatcccct tcgcgaaggg gatggataga aatagggagc
     2461 ctaggtgaga tatcctgtcc cctggcgttg tgtcgtgtca aaaatggcgg aaggagcatc
     2521 ctcgccacct tccgcctcgt cgcgctccgg ggccgtattg tggcggtgag gcgtgccata
     2581 tcttcctttc ctcgaggcct cgcccccgga ctccctcctc tattttttcc gcaaaacctc
     2641 tttgacgccc cgggggcggt gtgctacaag gggctggttc gggccggcgt tctttcgctg
     2701 cccggcgggg aggttccggc gtggagtcgt ctcttttcct ttttccccat ctttctgaag
     2761 accatcgccg tcggttcatc gccgccggaa cgcgccaggg gtggttccac cgtccggaac
     2821 cgctggtggt ggcgctttcc ggcggggggg attccatggc gctgctcctg ctgcttttgg
     2881 cggtgacgcc cgcgtcccgt ctggtgctgg cccatgcgga gcacggcctc cggggggagg
     2941 cctcccggga ggatgcccgg tttgttcagg ccgtggggcg ccagttccat ctggacaccg
     3001 aggtggcctt tctggcggtc cgggagaacc gttgccccgg cgaaagcatc gaggtgtgcg
     3061 cccggcgcct tcgcctacac tttctggaag agacggcccg gtcccgcggg gccctctgga
     3121 tcgccacggg gcaccaggcg gacgatctgg cggagaccgt gctctttcat caggtgcggg
     3181 gggcgggggg gcggggcagc gcgggctttc gggaggtgcg ccttccctac gtgcggccgg
     3241 tggtggattt cacccgggag gagctgcgga gccttcttcg ggcccagggg ttttcctggc
     3301 gggaggacgg ctccaacgac agcccggagt acacccgcaa ccgcatccgg cacacggtgc
     3361 ttcctctgtt ggaatcggcg gtgcatccgg gggccacgcg ccacctcgcc gccctggggc
     3421 gggagatggc ggagcgcgag gatcgggagg atggggaggc gggggccctg ctggcctggg
     3481 tgcggcgccc ctttccccgg gcggagcgcg cctggcgggg ggatgtggcc gtggggctga
     3541 aagaccatct gctggcgcgg ctgctacgtc tggaggcccg atctctgggg tggggggccc
     3601 tggaccgtcg ccgcacggag gatctgctct ccctggtgcg acgcccttct tcctgctggc
     3661 gttttcagtg gcaggaggac cgggagctcg tggggggaag gcccctctgg gggtgggtgc
     3721 ggcgcccctg gggggagccg ctgggagccg tgcctttgcc gccggagggg gagtggcgct
     3781 gggggccctg gcggtgccgg tgggaggata cggtggcgcc ggagggccgt tctttcggag
     3841 ggtggggtct ggggttgcgc ttttgtgctc ctccggagaa gagttttctg gtgcccctgg
     3901 ccagcctgga cggcgaaacg cggcgcaaag ctccggtgcc ccgctggtgc cacgcctggt
     3961 ggcccgcagt ggaggggccc gagggggcgc ggattctccc ctgggatcgg ggcgatcttt
     4021 cccttccacc ttcggggggc tgtgatagaa tggatcggtt gctctcgttt tccctaggaa
     4081 aggagtttgc ctgttcatga gctatgagtt gggccatccg atattggaag aagccacgat
     4141 ccgccatcgg gtccgggagc tgggggagaa aatttcccag gattacgacg gaaagaccgt
     4201 ggtctgcgtg ggagttctca agggatcggt cgtctttctt tccgacctgg ttcgggcggt
     4261 agacggccgc cgggtggatg tccgattgga cttcatggcc gtgtcctcct acgggaactc
     4321 cacggaatcc atgggagtgg tgaagatcct gaaggacttg gacacggaca tccagggatg
     4381 tcacgtgttg ctggtggaag acatcgtgga tagtgggctg accctctcct acctgaagag
     4441 gcttctgggg gaaagaaacc ccgcgtcgct acgggtctgc gtgttattgg ataagccgga
     4501 gcgacggcgc gtggaggtgt cggtggatta ccggggtttt gtcatccccg acgagttcgt
     4561 ggtgggatat ggcctggact atggcgggcg atggaggggg ctcccggcgg tatatcgggt
     4621 gacggtctag gggcgtcggc ataaacggcc cgggggcgga gaacaggaga tgggggcgcc
     4681 tgcgccgggg acggggatct tgagggtttt cggacggaag gatccgggga aaagaagggg
     4741 caaaggggtg gtcggagagt gaaccggctt gtgaaaaatc tcggggttta tctggtattg
     4801 gtggtgcttg tggtcagcgt ggtgaacgtc ttcctatcgc cggacaaggc atcccaggag
     4861 gcccaaaggc tcaattacag caagttcctg aagtcggtgg acgacggact ggtgcagaag
     4921 gttgtcatcg acgaggacgg catccagggg acgttggtga acggtacgcc ctttgtcacc
     4981 tatacggtgg gggtggggga cctggccccc aagctggtgg gaaagggcgt ggatgtggcg
     5041 gtggcgccgc ctcagaagac cccttggtgg accggcttgg tctccaccct gtttcccacg
     5101 cttttgctca tcggggcgtg gtttttcatc atttaccaca tgcagggcgg gggcaacaag
     5161 gtgatgaact tcgccaagag caaggcgaag ctcttcctgg acaaccgtcc caaggtgacc
     5221 ttttccgacg tggcggggtg tgacgaggcc aaagaagagc tccaggaaat catcgactac
     5281 ctcaaagacc ccgctcgctt taccaccttc ggcgccaagg tgcccaaggg ggtgctgctc
     5341 ttggggtccc ccggcacggg aaaaacgctt ctgtcccggg ccgtggcggg ggaggcggat
     5401 gtgccgtttt tcagcatcag cggatcggat tttgtcgaga tgttcgtggg ggtgggggcc
     5461 gcccgggtgc gggatctctt cgagcaggcc cggaggtatc agccgtgcat catcttcatc
     5521 gacgaaatcg acgcggtggg gcgacagcgg ggcgcggggc tgggaggagg gcacgacgaa
     5581 cgggagcaga ccttgaacca gctcctggtg gagatggacg gcttcgatac gagcacgggg
     5641 atcatcctca tcgccgccac caaccggccg gatattttgg atcccgccct gctgcgtccc
     5701 gggcgcttcg accggcagat cgtggtggat cgtcccgacg tgaaggggcg cttggcgatc
     5761 ctcgaggtgc atgccaagga caaaaagatg gggacggacg tggatctaaa cgtcattgcc
     5821 cggcgcaccc cgggatttgt gggggccgat ctggccaact tggtgaacga ggcctctctg
     5881 ctagccgccc gccgaggaaa gggcgcggtg gagatggcgg agatggaaga ggccatcgac
     5941 cgggtgatcg cggggccgga gcgcaagagc cgggtcatca acgccaagga aaaggagatc
     6001 atcgcttacc acgaggcggg acacgccctg gcggcgaagc tcattccggg gtgtgatccc
     6061 gtgcacaaga tctccatcat tccccggggg cacaaggctc tggggtacac gctccagatc
     6121 cccgaggagg accgctatct ggtttcgaag aaggaacttt ccacgcgcat ccagagcctc
     6181 ttgggggggc gagtggcgga ggaaatcatc ttcaaagacg tcaccacagg ggctcagaac
     6241 gacctggagc gggccaccct cattgcgcgc cagatggtga cccagtttgg catgagcgag
     6301 cgactgggcc ccgtgaccct ggggcgcaag cagcacgagg tctttttagg gcgcgacatc
     6361 gtggatgacc gcaattacag cgatgaggtg gcctttgcca tcgatcaaga ggtgcgacgc
     6421 atcatcgacg aggcctatga gggggtgcgc gccctcttcg tggaccatcg ggatctgttg
     6481 accaaaatcg ccgagaccct gctggaccgg gagatcatcg aggccgagga gttaaacgtc
     6541 ctcatgggta acggagccga ggagcccgct ccggcggagc catcgccgga gcccgccaag
     6601 gaggagccga cggcggagcc cttccgctac cctcgcccca ccccgcgagc cccgggagag
     6661 gtttccataa agcccgcctg accccgggag agggccccaa aaggggcggc ccccattccc
     6721 gagggtcaac gacgggagga tctgcgcgct gcgagtcttg gcagcgcgct ttgtttcttt
     6781 ggggggaatt tttatggaag agcccgaccg atttcttctg gagtcaccct ggcccccctc
     6841 gggggaccaa cctcaggcca ttgaggcgct ggcgcggggg gtggagctgg ggcaccgttt
     6901 ccagacgttg ctcggggtga cggggagcgg taagaccttt accatcgccg ccctggcggc
     6961 tcgcctccat cgccccgtgt tggtgctcgc ccacaataag accctggcgg cccagcttta
     7021 cagcgagttc cgggggtttt tcccccacaa tgcggtgcac tacttcgtga gctactacga
     7081 ctactatcag ccggaggcct atgttccagc ccaggatctt tacatcgaaa aggacgcctc
     7141 cgtgaacgat cgcatcgaaa aactccgcct ggccaccacc aaggctctgc tggagcgccg
     7201 ggacgtggtg gtggtggcga gcgtatcgtg tatctacggc ctgggacaga ggaaaagcta
     7261 cgaagacgcg gtgttttcct ttgctgtggg ggataggtgg gagcgccggg ccttcttgga
     7321 gcgcctcatg caaaactact acgagcggaa cgatgtggcc ttctccccgg ggctctttcg
     7381 ggtccggggc gacgtgctgg agatctatcc ggcctacagc gacacggcgc ttcgggtggc
     7441 gttcttcgac gacgaggtgg agcgcataga cgaaatcgaa cccgtctctg gtcaacgggt
     7501 gttgagcaag atgaaggcct ctatatttcc cgccaaacac tacgtcacgg ggcccgatgc
     7561 catcaagaac gccatggaac acatcctggc ggacatggaa aatcaggcgg cgaagtttca
     7621 gcgggaggga aagctcctgg aggcccagcg cctggtcact cgcaccaggt acgacatgga
     7681 gatgctgacc gaggtggggt actgttccgg catcgagaat tactcggtct atctggatgg
     7741 ccggagcccg ggagatcccc cggggacctt gctggacttt tttcccaagg atttcctgat
     7801 ggtcatcgac gagtcccaca tctccgtgcc ccaggtgcgg ggcatgtaca acggcgaccg
     7861 atcccgaaaa gaggtgctgg tggactacgg ctttcgcctc ccctcggcga tggataaccg
     7921 tcccctgaga tgggaggagt tcgaggaata catgcaccag gtgatcttcg tctctgccac
     7981 accgggggat tgggagatcg cccagtcttc ccaggtggtg gagcagatcg tgcgccccac
     8041 aggggtggtg gacccggaga ttctggtctt gcccgcccga agccaggtgg atgatctggt
     8101 ggggaggctt aaggacgtgg tggaacgggg ggagcgggcc ctggtcaccg ccctcaccaa
     8161 gcggtcgtcg gaggacctgg cggagtacct gggggacctt ggctttaagt caaaatacat
     8221 ccattctgag ctggacgcct tcgagcgggc ggagctgatc cgggatcttc gcctggggag
     8281 gatcgatgtc ttggtggggg taaacctgct tcgggagggg atcgacctgc cggaggtctc
     8341 tctggtggcc attttggatg cggaccggga gggtttcttg cggtcggagc gatccctggt
     8401 gcagatcatc ggccgggccg cgaggaacgt ggcgggggtg gtgatcctct acgccgatca
     8461 ggatacgggg agcatccaaa gggccgtggc ggagacagat cgccgcagga aaatccagca
     8521 agagtataac gaaaagcacc acatcacgcc ccagtcggtg cgcaaggggg tcggtcactt
     8581 gctccccgag gagctcctgg aaaaggggac ggaaggatcg gaggaaagcg acgaaatcat
     8641 gccccgggag accttggagc gcatgatgtg ggaggccgtg gaacgcctgg atttcgagcg
     8701 ggcggcccag cttcgggatc aattggcggg aaaaaagaaa ggagccaccc ttcgtgtcgc
     8761 aagtcctcgt cgtgcgcggc gcgcgcgagc ataatctgaa aaacgcccat gtgacgatcc
     8821 ccaaggatcg cctggtggtg ttcaccggcc catcgggatc gggcaagtcc tctttggcct
     8881 tcgataccat ctatgccgag ggacagcgac gatacatcga atccctgtcg tcttacgccc
     8941 ggcagtttct cggagtgcag gggaggcccg atgtggaaga catctcgggc ctttctcccg
     9001 ccatatcgat cgaacaaaag ggacgcagcc acaacccccg ctccaccgtg gggacggtca
     9061 ctgaggtcta cgattacctt cgtctcctct ttggccgcat tggggtgccc gcatgcccct
     9121 cctgcggcaa gcggctctcc cggtacacgc tggacgagat cgtggatctg atcctgcggg
     9181 attacgacgg gaaaaggatc gaaatcttga gccccgtgct gcgggggaaa aagggggagc
     9241 accgcaatgc cttccaacag atccgccaaa agggttttct gagggtgcgg gtggacggca
     9301 cggtcttttg gctcgaagaa gagatcccct tggacaagaa caaaaagcac accgtggaga
     9361 tcgtggtgga ccgcctctcc gcccgggagg accggcggac ccggatgagt gaggcggtgg
     9421 aatcggccct ccgggagagc aagggttttg tcctgctcct gcccgagggg gaaaaagagc
     9481 gcctcctggc ggagaacttc gtttgcccgg agtgcgacgt ggcctttccg gagatctccc
     9541 accgcctctt ttccttcaac aacccggcgg gggcctgtcc ggactgtacg ggcatcggaa
     9601 gccacgaaca cttttccgaa gagctcgcgg tggatcccga tcttaccatc gcccaggggg
     9661 ccctgctccc ctgggaggga acgcactaca tgttgcgtcg cctccaggcc ctggcagagg
     9721 ccaagggctg ggatttgacg ctcccctttc gggagctttc ccccggtctg cgagagagca
     9781 tcctcgaagg agaccccgat cgagtcccca tgacctacaa agagcacggg gaagagcatg
     9841 tctaccaggg gcgtttcgaa gggctccttc cctggctcac ccgccgctgg gaggagacgg
     9901 aatccgaaaa cgtgaaggag gacctgctgc gatttcggat ggagaaggtg tgccccagct
     9961 gcggggggct gcggctgcgc cccgaagccc tttccgtctt cgtgggggga aagaacttgg
    10021 gggaattggt ttcccttcct ctggacgccc ttggggcctt cctccgagat ctgacgctga
    10081 attcctggga gcaagacgtg gtgcgccagg tgatggacga actaaacaag cgattgagtt
    10141 ttctcctcga cgtgggggtg gaatacctgt cgctctcccg ccgggccgat accctatcgg
    10201 ggggggagag tcagcgcatt cgcctggcca cccagatcgg atccaagctc acgggggtgc
    10261 tctacgtcct cgatgaaccc accatcggcc tgcacccccg ggacacggcc cgcctcctgc
    10321 gcaccctggg caccatccga gacctgggca atacggtgtt ggtggtggag cacgaccgag
    10381 acaccatgaa ggcggcggac ttcctggtcg aactggggcc tgcggcgggg gagcgggggg
    10441 gagagatcgt ggcggtgggg accccggagg aagtgatgtg cctttccacc ttcaccggtc
    10501 cttacctcaa gggggagacc tgtggggtct ttcgccctta cgccgagagg cgccgccccc
    10561 gggaagagcg gctcgtggtg cgcggtgccc gggagcacaa cttgaaggac ctggacgtgt
    10621 ccattcccct gggggtcttt tcctgcctca ccggagtgtc ggggtcggga aagagcacgc
    10681 tgatgcacga agtgatctac aaagggttgc ggcgacgtat ggaccgatcg tttcgcggcc
    10741 gggcggggga tcatcgcgac atcgaggggt ggcaaaagct gcgccacgtg gccctggtgg
    10801 accagagccc cataggccgg accccccgct ctaacccggc cacctacacg ggggtcttta
    10861 cggccataag ggagctcttt gcccaacttc ccgaggcgaa gatccggggg tacgccccgg
    10921 ggcgctttag cttcaacgtc cgaggaggac ggtgtgaggc ctgcggtggt gatggggtgg
    10981 tcaaggtctc catgcttttc ctccccgacg tgtacgtcac ctgcgatgtc tgccgggggg
    11041 cccgatacaa ccgggagacg ctggaagtgc gctacaaggg gcgccacatc gcagagatct
    11101 tggacctctc cgtggaagag gccgtgacgg ttttccaaga catccctcgc atcgcgggaa
    11161 agcttcgggt cattgaggag gcggggctgg gttatatcag gctgggccag tccgctacga
    11221 ccctctccgg gggagaggcc caacgcgtca agctttccac ggaactttcc aagcgttttt
    11281 ccggcaccac gctctatctg ctggacgaac ccacgacggg gttgcattat accgacgtaa
    11341 aaaagctcct ggtgctgcta cacaagctgg tggatcaggg caacagcgtg ttgctcatcg
    11401 agcacaacct ggacgtcctg gcctcggcgg attacatcat agacctgggc cccgaaggag
    11461 gggcgggggg aggaacgctg gtgaccgagg gcacgccgga agaagttgcc cgctcaccgt
    11521 acggccacac gggaaggtgt cttcgggcct atctccagga aatccgagag ggaggaatgg
    11581 catgagtcgg ccatcgcggc gtcccgcgcc tccgcccgaa atcccggagc gggggagggg
    11641 cctcgaagac gagggttggt gctggggaaa gcaccccgtc atggccctgc tccgggaatc
    11701 cccggcgcgg gtgctgaagg tgcttctcct ttcccgggtt gggggatggg accccgtcct
    11761 cgggctctgc aacgcccacc gtatccccgt gatctgggcc gatggccccg cgctggatcg
    11821 tcactgcgac ggaggtgccc atcaagggat cgtagcccag gtagcccccg ggccccttta
    11881 cgccctggaa gagttgccgt cccttctccc cgaagaaggc cttccggccc ttgtggtgct
    11941 gctggaccat ctacaagatc ctcacaacat gggggccgtc atccgtacgg ccgaagccgc
    12001 cggagcgaag gccgtcgttt ttcccaaaag gcggggcgtc cttcccgggg gaacggtggc
    12061 caaggccagc gccggagcgg cgttacgcct gcccctggtg gcggtgacca acctgtcccg
    12121 ggcgattcag gagctgcagc ggtggggttt ttggagcgtg gggttggaag ccgccgctcc
    12181 ggaggggctc tggagcccct ctcccctacc ccgacgcctg gccttggtgg tgggatcgga
    12241 gggagaaggg ctggctcgcc tggtggcggc cacctgcgac gagcttcgat cgatccccat
    12301 gcgaggcgag gcgggatcgc tcaacgccag cgtagccgcc gcgctgggca tgttcgagtg
    12361 ggtacggcag tggggataaa gttccccggg gatttttcca cagggaaggg gcctaggggc
    12421 ggtcttggtg cttggtggcg aagggacacg gcgaaaccgt tgacatccta tggggcttcg
    12481 cggtagaata cgggccgttg cggtcgtggg tggttagttc agtggaagaa cgcttccctg
    12541 acacggaaga ggtcacaggt tcgaaccctg taccacccac cataaagtaa cggcaagggg
    12601 ttccgttccc tttcaccggg cggaagcctc gccagggaag ggatggatcc agggtagaag
    12661 ccgtcccctt cctagggcaa aggttccgtg gcgggatccg ggggtgcccg tggggccctt
    12721 cggacctttt tccgccgacg gggccgatct tggggggtag gggaggaagt tttcctccgg
    12781 gggctagact ggaggaagct ttccgggtat aattcccact tgtcacggaa ggcatcgatg
    12841 gctggcccgg gggccacaga gaagcctctt tcctttggcg gcgcttccct ttcagagcgc
    12901 catccttcgg tggagggacc tttttctccg ggttcggggc tttcgctggg gcgtatgggc
    12961 ggggcgtggc gcaatctggt tagcgcacct gctttgggag cagggggtcg aaggttcaaa
    13021 tcctttcgcc ccgaccagaa aaggcaatga ggagcgtgcg ggaatagctc agttggctag
    13081 agcgatggcc ttccaagccg taggtcgcgg gttcgagtcc cgtttcccgc tccatagatt
    13141 gttccttgtg cgcctgtagc tcaactggat agagcaacgg acttctaatc cgtaggtcgc
    13201 gggttcgact cctgccaggc gcgccacatt gcgaaagcgg tcggtgaaat gtgaggcagt
    13261 tatggtgagc gtagctcaat tggttagagc gctggactgt ggctccagag gctgggggtt
    13321 caagtcccct cgttcacccc attacaattg agcgcccgta gctcagtgga cagagcgtcg
    13381 gacttcggat ccgttcgtcg cgggttcaaa tcctgccggg cgcgccagca agggaacgcg
    13441 tggatcgtta gctcaatagg cagagcacct gactcttaat cagggggtta taggttcgat
    13501 tcctatacga tccaccattt tttttgtttt ccccacagcc aaataggaaa gcgcgagcgc
    13561 gtggatcgtt agctcaatag gcagagcacc tgactcttaa tcagggggtt ataggttcga
    13621 ttcctatacg atccaccatt ttttgtttcg cggtgcgcga gagtggtgga attggtagac
    13681 acgtaagact taggatctta tgcctcggcg tgggggttcg agtcccccct ctcgcaccag
    13741 gcgaccaaac gccccgaagg ggtgcggcgt gtgaaagatg gtaggtgatg ggctgtcact
    13801 caagaagggg tgacggcctt tctttttttt cgagaaaata ttaaaatttg ctggataaca
    13861 agcgaggggt ttgacgtcgc gttgtcgcgg cggttcttcg ttttccgtgg cgatttttcg
    13921 ggagccattc ctcggacagg aggcgcgata aaatgtcggt gcaggatcaa agttgtccct
    13981 ttcttctcga aggacaatta cccgcaaggt ggcgacggga agttgacgga gctctccggg
    14041 ccgcgtgctg ggcgggggcc ttgttgcgcg aagaggcgga tcgtcccggg gggccccggg
    14101 gcagtggggg ccacgcggag atcgatgaag aggtggagtg gggaatccat gatttcatcg
    14161 cggagaactt ccccgaggat gggtggctgg gagaggagac gggagtccgg atacgccccg
    14221 gaacctccgg gcgcgtctgg gtggtggatc cgaacgacgg cacccgggag tttcttcagg
    14281 gctttcgggg gccagcggtc tccgtggccc tggtagcgga ggggattccc gtcctggggg
    14341 tggtctatgc ctataaccac cccgacgacc ggggggatct gatcgtgggc gtggtggaag
    14401 gggggttcgt cttccgaaac ggtgtccgag tggaacgtct gtggcgcgaa attccttccg
    14461 acgaggccct ggtcattgtc gcccccggag gagagaagaa ccccgtggca aatttgcagt
    14521 gcgcccaccc tctgcgcttt cgcgtcatgc ccagcatcgc ctaccgtctg gccttggcgg
    14581 cggtggatga gggtgacgtg gggtttgcgc tgatgggtcc cgtgtcctgg gatgtggcgg
    14641 gggggcacgc cattttgctg gccgccggcg gtgatctcta cggggtctcc ggcactcccc
    14701 tgcgctacga ccgccggggg gagtttccag gccagagcct tttgctcggg gggccccggg
    14761 ggctggtgga gcatcttcgg tcgaaggact ggtcccgtgt gacatatccc aaaagtgatg
    14821 ggagtccgtc ggccctctgt cgtcctctgc ggagcaggga agctctggat ccccatgttc
    14881 tcgcccgggc ccaggggtgc ctgctggggc agttggcggg agatgccctg ggcagcctgg
    14941 tggagtttga atctccggaa gatatccgtc gcctccatcc cggaggcgtg cgagacctcg
    15001 tcggtggggg cccctgggga accatcgcgg ggcaacccac cgacgactcg gaaatggctc
    15061 tggctctggc ccgcaccctg gtggccgacg ggagttttcg ggtcgatcac gtggcccagg
    15121 cctatcgcgc ctggcttgct tcggatccct tcgacgtggg acataccacc cgtcaggccc
    15181 tcctggggga gccccaaccc gacagccagg ccaacggcgc cctcatgcgc ataagtcccc
    15241 tggggatctt cggtgccttt tgttcgccgg aggacgtggc gttctgggcg gcgcaggatg
    15301 cggtcctcac ccatattcat cccatttgtt cccaggcgag cgcccttttt gcccgtgccg
    15361 tggcccacgc cattcggaac gggggaagcg gcgaagggct ctttgaccac atggcccgct
    15421 gggcggagga gggggagctc gacccggcga tccgccagct ctttccccgg atcgcccgga
    15481 gagagcgtcc cgtcgagacc gttcatgccg gatgggtgct cgtggccctg cagaacgccc
    15541 tgtggcatct gcgccattcc tcctctctgg aagaagctct ggaagacacc gtcatgcggg
    15601 gcggcgacgc cgacaccaac gccgccatct gcggagcgct gctcggggcg gtctttggcc
    15661 gggacggggt accccaacgc tggcagaagc gtcttttctc ctgtcgccct gcggcggggg
    15721 tgcggggagt tctccatccc cgcccggact gctactggtc cgtcgatgcg ttgatgctgg
    15781 cggagcggtt gctgcgggcg gggatggggc ggtaaggccc gacgtcacga ggtcctgcgg
    15841 ggtgttgtgg gtcttccatg gccccggagg ggagcaccgc gtcgaaaaaa ttggccgttt
    15901 cccgaaaaac gggggcggct tttgtttttt tctccaaaaa cgatatgctt cctcttagag
    15961 gcatgtctcg gtacgaagtg aggaaggtgg ggatgtttgt tgaacgttca gcagggtttg
    16021 gtggcgttgg cggtgctggg gggcggcgtg gggggaaacg ccttgggcgt tcctgcgggg
    16081 tatcttgtgg ggggctttgt gggggggctt ctggccaagg gggccacggg gcttgacctt
    16141 cttcccggag gggaggcggt gaccgtggtg gggcaggtgc tggtggccta cgccatggtc
    16201 tgctcggcgg atctctcggc gtggcgggcc tttcctcgtc tgttgcctgt ggcggtgctt
    16261 tccgctgcgg tgctcacggc ggcgagtctg gtgttggcca aggtgctgga aaaagtttgt
    16321 catctggacc ccatgacggc gctcttttcc acgccccccg gagggctcac cggtctggcc
    16381 gtggcggggg cttccatggg gagcgatagc cccatttccc tgctctttca gatggtgcgc
    16441 ctcatcttcg tccttttggt gattcccttt gtggcttcgt ggtggacgcg atggtaacgc
    16501 cccaggacgc ttcggcggcg aaatcttccg aaaggggtga caggttgctg tggggggctt
    16561 tgactttcat gctctttacc tggtcttcgg cctttcccgc catccgcatc ggcctatcgg
    16621 ggttttcccc cacccacctg gctctgtttc gcttcctggt ggcatcgggg gcgttgatcc
    16681 tctacgccct ggcggtgcgc atgcccctac cggagatgcg agatgtgccg aaattggcgg
    16741 ggttggggtg ccttgccatc accctttatc acatccctct gaacttggga gagatgacca
    16801 tttcggcggg ggccgccagt ctattgatga gcacggcgcc ggtctgcacc gcccttctgg
    16861 cggcggcgat cctgggaaac cccttttccc gaggaggatg gatcggcctg ggggtgagct
    16921 tcctcggggc ggggctcatt gccctaagcg aggggggatt ggggggcttt tccgtgggag
    16981 ccctgtgggt cttgctgtcg gtggtctcag agagcctggc ctttgtcctc caggggggga
    17041 tgctgaaaaa gtatggcccc ctgaagacgg ggacctatct catgtggacg gggaccgcgt
    17101 tcatgctgcc cttcggaggg gggcttttcg atgccatcgc caaggcccct tgggaagcca
    17161 cggcggtgac gatctatctt ggccttttcc ctgcggcgtt ggcctatctg tgctggtccc
    17221 tggtgctggt ccgactcaag cccgcccacg ccacggccac gttttatgcc ataggaccct
    17281 tggcgctttt gatctcttgg ctgtggatcg gggaaaggcc ccttcctctg gccatcggcg
    17341 gaggggcctt ggccctcttg ggggtgtgga tagtggaacg gagtcgccgg acttgacctt
    17401 tctgacggtg caggccctac tttttgtcgt gctgatggtg gctccggcgg gagcggccct
    17461 ggtgctcttg aaaagacgtt cccgccggcg aagcctttga atccttgggg cccccggagg
    17521 gcctcgggat ctccggagga cgacgatccg gcccacaggg cttggctttg cgcggagcac
    17581 ctttcctcct ttcggtgatt tgcgaggctt gttccgggcg atttttcgcc cgacgagacg
    17641 gtttttttgt tatttgtcga cggcggaagg cgttaggggt atacaaaagg gcgaaaggag
    17701 tgtatattac cggggtagat ggaaaacttc gggacccttt gccccggagt tcatatcgcc
    17761 catacgaacc ttcgacccgg tttgcctctt gtaggggagc cgggcaatgg gtgggaggag
    17821 cgatcctttc gcctgccgcg tttgcgaaga agggagaccc cagggggtct cccttcgcgt
    17881 attttttggg ggcaggtcgg cgcggcctgt cggagggtgc ggattttgcg gcggtgtggg
    17941 gttggggggc gttccacgtc cttgccttgg gccgctggcg gagggtttgc ccgacggttt
    18001 cgccgggggg accctcggcc tgcgggggat ctttgagaga tgggcccatc gtgatccatc
    18061 tcggaagtga gggggtgcga tgtcgaagcc taatcacgga agcgctgttt tatccccctg
    18121 tcctgggggg gctgcccgga cggatatacc aaaacaaaaa aaggaggcac aaagaccatg
    18181 ggaaagttcg gtgacatcct gcgcattgat ctggcgaaaa agagcgttac ccgggaagac
    18241 gtagacgaaa atcagcttcg ggactacatc ggcggtcggg gttacgccac gaagatggtc
    18301 tatgacgagg ttcccaaggg caccgacccc ctttcgccgc agaacaagct catctttgcc
    18361 tgcggccccc ttaccggtac gagctcgcct gcgagcggga gatacatggt ggtcaccaag
    18421 agccccctca cggggtatat cgccagcagc aactccgggg ggttttgggg ggcggagctg
    18481 gccaaggcgg ggatctttgc ccttgtcctc gaaggcaagg ctgacgcccc cacctatctc
    18541 tggataaaag atgggaaagt ggagctccgc gacgcgagca aggtgtgggg caaagacggg
    18601 catgagacca ccgacatgct gcttgccgag gtcggcgacg acaaggcccg ggtggcctgt
    18661 atcggcccgg cgggggaaaa gctttcactc ctggcggcga tcatgaacga taaggatcgg
    18721 gcggcgggac ggagcggtgt gggggccgtc atgggttcga aaaatctcaa ggccgtggtg
    18781 gtgcgcggca cgggcatgcc ttacatgcac gacgttaatg ccctccgcga cgccctccgc
    18841 gatggtgttc acaagctaaa ggagaatggc gtcaccggca aggggctccc ggcctacggc
    18901 acggcggtgc ttgtcaacat catcaaccag accgggggat atccctatcg caactggcag
    18961 gatgcctaca tggaaaaggc cgattcccag tcgggagaga cgctggccga taagtatctc
    19021 aagaaaaact ccgcctgttt cggttgcccc atcggctgcg gccgcgtcac cgaagtggat
    19081 ggcaaaaagg gcgaaggccc cgaatacgag agcatctggg cctttggcag ctgttgcggc
    19141 gtcgaggacc tcgagcccat attgcgcgcc aacgtggtct gcaacgagct ggggatcgac
    19201 accatcggcg ccggagtcac cgtggcggcg gccatggagc tgtacgaaaa gggctttatc
    19261 cccgacggag acctcggagg cggcccgaaa cccctcttcg gagacgggga tgccatggtg
    19321 cattgggtca ccatgatggg taagggagag acccctctgg gcaagaggct cgccctggga
    19381 tcctatcgtc tctgtgaagc ctacggtcat cccgagttct ccatgagcgt caagaagcag
    19441 gaaatgcccg cctatgatgg ccgggcgatt cagggaattg gcctcaccta tgcggtttct
    19501 aatcgggggg ggtgccacgt gcggggctat ctcatatctc ccgaggtgct ggggataccg
    19561 gataagctcg accccgaaga tatcggcgcc aagccacagt gggcgaagat cttccacgac
    19621 ctgtcggcgg ccattgattc ggcgggactc tgccttttca cctccttcgc cctgggggcc
    19681 gaggactacg caggttatct gagggccgcc accggctttg actacaccgc cgacgaagtc
    19741 atgacctgcg gcgaccggat atggaccctg gagcgtctct ttaacctgcg ggaggggctc
    19801 accgccgccg aagatacgct gccctcccgc ctgcgagaag agcccatccc ctcgggcccc
    19861 atgaagggca aaacgagccg ggtcgcggag atggtgccgg agtactaccg gctgcggggc
    19921 tgggatgaaa agggtgtgcc cacgccggaa aaactgaggt ccctcggcat atagcggaaa
    19981 tgatgccctc cggtgagggg taactccagg ggcttggaag gcgaagcccg ccccgggcgg
    20041 ggatggcccg gggcgggaca cgtttcccga gggggtgggg ccgctgtgaa ggtgctgttt
    20101 tttggatttg tcagggatat gacgggcgag aaggaaaccg ccgtggaggg cgcggcgacg
    20161 attatatccc tttttgaagc actggccacg cgctacgggg agcgctttcg taccgaagcg
    20221 cttcccgaag gggagctgtc gagcgagctc atcgtcctgg tgaacggtcg ccacatagcc
    20281 cattccggcg gcgcggcgac gccgctgcgg gaggaagaca ccgtggcgat tttcccgctc
    20341 gtggggggag gataaaagcc tccccctttt ttcatgggta gaatgccggg gaagtttacc
    20401 gttgttctcc aggacacctt gaaaaaacga tcaccatgat gtatccctct ttcaggaggt
    20461 ggggtggcgg cagacctttt caggaaagag gggataaccc gtgagaaacg ataccggaag
    20521 gcggagcccg tcccatctaa gcatcgtcgc aggccccggg ggagggtctg gtccgatgga
    20581 attccccgcc gccgagcttc cagggagaga gataaaaggc gttcttttag aaaaactggc
    20641 cgaggccccg gagcgagcct tggcctttgt cgtgccaatg gttgttccgg cggaggcgat
    20701 gcgggagcct ttgggagggc gtcatcatcc atgaagctct tgggcctttt agctccttta
    20761 ggggtctttg gggcttatac gctggcggtg atcctgctta ccggccccgg ctttgtctgg
    20821 aatgagaacc gccgtcgttt ttatctggcg gggcgagggg cggggctctg ggcctctgtg
    20881 gccacttttg gcgccacgtg gatgagcgcg gcgtctctgc tgggttatcc ggaatctttc
    20941 ttctgggatg gcatggtggc ctttggaggg gcggtgaatg gctggcttct ggggcttttg
    21001 cccttgccgt ggatcgtggc ggtgttacgc cgatcccggg ccctttctct gccggagtgg
    21061 atcgggaaga ccttcgacga tcctcggctc cgtcgtttga cggcggcggc gcttctgttg
    21121 gcttatgttc tgtatattct catccagttt cgatccttcg gaggggcggt gtcccatctg
    21181 ctgggcattc gttacgccgt gggggcctcc gccctggtct atctttttgt gctctacacg
    21241 accttcgggg gcatgccttc ggtgattcgc agcgatgtgc tgaatttctg gctcatcgtc
    21301 gtgggagtga cggtggcagc ggtggtggcc gtgggtcgcg tgggcggcgt ggaggtcctg
    21361 gtccgacgct tggcgacgga ggccccagag cttttgaatc ccgccgcgcc gtgggaagtg
    21421 ctagggggta tggcggccat gacgctcgga tgggggctgg gagtggcggc aaatcctcag
    21481 tacgccgtgc ggatcctctc cgcccgctct cctcgggtgg ccctggggat gctggccatc
    21541 gtgccctttt ttctcggggt gatctatctg ggacttacgg cctttgcggc gacggggcga
    21601 ctggtggcgg ccccggtgct tcgtcaggga ggagaggcgg cctttctcca catggtgagc
    21661 atggtgttgc cccctgcccc tgcgtcgctc tttttggtgg ccctcatcgc cgcagcggtg
    21721 agcacggcga attctcagct gctgttggcg gcctcgtctt tttgttatga tctgatggac
    21781 cgatggagaa ctacgctgcc ggaggacatc ttcctgttcc gcaaccgtct ggcggtgacg
    21841 gtcatcgcca ctttggcgct gggaggaagt ctgttgccgt ttccagacat cctcactgtg
    21901 gggcgcttca gctggaccgt ggtggcgctt tgcttttttc ttcccctctt ttggcccctt
    21961 tccgggcgtc gccaggggct tttcgaggcc atggggcttt ctctcgtgct ccacggagtg
    22021 ttggtcttcg ggttgcacct accgcccgag ggatccctgt tgccttcgct cttgctggaa
    22081 cgtctgtcgt ggcggtggtt ttctcggaag ggggaggagg cctgacatgg ggcggccgcg
    22141 acacatggaa agccagataa aggctttggt ggtggcggga gtgcttttgc tcacggcggt
    22201 cacgctggcg gtgtcgattg gccagggatt tgcccttagg tgggaggaga accggagatg
    22261 gctatatacg gtacgatcgg tcctagaggc attggatcgc ccccaagcgg aaatagtggt
    22321 ggccatgctc cgaccggcat ggagggtttt tccgggaacg gcggggagat cggggggaga
    22381 gatcttgcgc tcccgggatg gggagatgtg ggcggcgttc cctaacgacg aagtcctgtg
    22441 ggaggagttg ttggagagca atcgacccct tctggtggtg gcgctggtga cgctgttgct
    22501 cgtgttggag atatcgattt tttgggcgta cgccatcgtg cgacccctgc ggcgcctggc
    22561 atgggcctgt cgtgatatgg gatcgggccg gtggcccgac ctggggccgg agggggcgtc
    22621 ggggacgtat gagatcgcca cgctccacga ggcctttcgt catatggtgg accgcctcca
    22681 gcagtggcaa cgggtggagc gacaggtggg acgcatggag cgactcgctg cgctgggtca
    22741 ggtggtggca ggggtggccc acgaagtacg gaatcctctg gcgtcccttc gcattcacct
    22801 tgatctattg gaggtagacg tgctcccggc gggacaaccc tcgctacgcg ttcttggcga
    22861 tgaagtggat cgattaaacc agatcgtgtc tcaacttttg gccttttccc gcccccgccc
    22921 ccccatagga ggaccggtgg acctgggaga ggtggtgtcc tggtgccacc agatggtgcg
    22981 agatcgcatg tcgcggcagg gggtttcttg gcgagagacg ggcttcgacg gggcaagacc
    23041 agtgccagga gatgcggctc agttgaggca gatgcttttg aatctggtgc tcaatgccct
    23101 ggatgccatg cctcggggcg gagagctcca tgtggggacg cgctacggag aagacgatgg
    23161 gccggggggg gtgcgccgag cggcgctgtt tttgagcgtg gaggattccg gggagggtat
    23221 agcttcggag ctcctgccca gactttttga tccttttttt acgacccgcc ccgagggtac
    23281 tgggttggga ttggcgttgg tgcaccgcat cgccgagctc cacggtggag cggtggatgt
    23341 ggtttcatct ccggggcgga caattttcac cgtcacgttg cccgagagag aaggagacgc
    23401 cccatgatgc gcatgtggat tgtggaggat gaggcggggc tttcccaggg actcgcccgg
    23461 gcctttgagc gcgacgggta cgcggtgcgg gtgctggacc gtctggggga ggtgcgcggc
    23521 gccttggccc aggaacggcc ggatgtggtg ctcttggaca ttcggcttcc ggacggagat
    23581 gggttggccg ccctgcccca tctgctccgg gaggccccgg aggcgcgggt ggtggtgatg
    23641 accgcctttg gagattcgtc cctggtggtg cgcgccatcc gggagggggc gtggaattat
    23701 ctggacaaac cctttcctct ggaggcggcc cgcaacatgg tttcccgggc ggtggagtcc
    23761 ctggagcttt cccggcgggt ggcccgtctt cggggggagt cccttccgct ggtggggagt
    23821 tccgctgcca tggagagggt ccgggccttt gtggccaagg tggccccttt tcgggatctg
    23881 aacgtgcttc tcatagggga aagtggtgtg gggaaggaag tggtggcgcg gatgttgcac
    23941 gctgcttcgg gggctcgggg ggatttcgtg gccctgaact gcgcggcggt gcccgaggcg
    24001 ttgctggagt ccgagctttt cgggtaccgc cggggggcct ataccggggc cgggcaggac
    24061 aaagaggggc tggcggagac cgcagcgcag gggactttgt ttttggatga gatcggcgac
    24121 atgcctctgg tactgcaggg gaagatgttg cgcttcctgg aaacgcgtac cctgcgcccc
    24181 ttgggatcca cgaaggagat ccgtctgtcg ctacaggtta tggcggccac ttgcgtggat
    24241 ctagagcgcc gagtggccga tggaacgttt cggcgggatt tgtattttcg aatcgccatg
    24301 ctaccgctgg tgattcctcc gctacgggac cgagaagggg atgtgaggga acttctggag
    24361 tcgtttttga aggagttcgc cctgaaactt aatcgacctt gcctccaggt cactccagag
    24421 gtggaagagg ccctacggca ctacgcctgg ccggggaacg tccgggagct tcgaaacctg
    24481 gtggagcggc tctatatcct tagggataga gaagatgggt tggtgcgtct cggggatttg
    24541 ccggaggaga tgttggagcc ccttccccag gagtgccttg aggggatccg cgaaggggga
    24601 ctggtccagc ggctcgacga ctacgagcgc cgcctcttgc gggaagcgtt ggaggaggcg
    24661 gggggcaatc gcacccgggc ggcctccgcg ctgggaatat ctcgattcgc cctgttgcgt
    24721 cgcctccagc gccatgccct cgaataggcc ttcgcttctc cagggtcccc ggctgacgac
    24781 ggccctgctg cccctgcctc tccttttgcc ccgtatagcc ctggcttcct ctccgctgga
    24841 agggctcacc cgggatctag aagacttgct ggcttctaac cctctggtga gagtgatccc
    24901 ccccgggggc gagatgggag actcctcgga gagcgaagag aacgatgagg agaggatccc
    24961 ggaagatctt ctttcgctgg acgaccactt gcgtaggcaa ctcgccgagg atcccctaga
    25021 tggttttgat ttcgactcat cccagctggg ggagacccta gacccccggg gatatctcaa
    25081 cgtgcccctg gtgccctggg gggttcatct cggcctttcg ccggaggaag ccccccgggc
    25141 tctggcggcg ttgcaggagt gggcggaccc tccggggctt tttgcgcgag acttgacgga
    25201 gtgcctgctg attcagcttc gccggaagct tggtgaggag ggagatgggg ggcgtatcct
    25261 acgggagggt cgagaggacc tggtacgggg agaccagcac accctggcgc gccgtttaga
    25321 atggccttcg gagcgccttc aacgggcgtt gaaagagctc caggccttgg atccccatcc
    25381 ggggtacgct tttcgcaaga cggtgatggt acggccggag ctctttttcc gccctttagg
    25441 cccggggaag gagctttccg tccatctggc ggaagaaaac ctgcctcggc tggcgctgga
    25501 ggcaaagggt cgctccccag gggagagcgg ctacgatcac gccgcttggc acgaggctcg
    25561 cgccgtttta ggggccctgg ctcagcgcta ccggggaagg gtggaggtgg gaaggtttct
    25621 ggcgaaacga caacgggcgt acctcatggg agatcttccc gcgccggagc ccctggtgtt
    25681 ggccgatctt caccgagaga tgggttatca tccgagcacg ctctcccggg tgctacgctc
    25741 cacctgggcc gtcacgcccc gggggacggt gcttttgggg caccttctct cccggccgct
    25801 ccaagggcgc ccggatcttt ctgtggcggc gttgcggcac cttcttcggc aagcccggca
    25861 cgaaggacgc tcctgggcgg aggtggcccg ggagctggcc ctttctcccc ggacggtggc
    25921 ctggcacggc ctccggtgcg gggggtaggg gtgcggtttt gatacgtccc ccgtgcggaa
    25981 agcgcacggt tttttgggac tctggggaga aagggaagat gcctcgaaag cggcggggct
    26041 ttcgggcaag ggaaaggagg acggggcgga aaaatatccg gggtgctctg cggcggcaac
    26101 ttttttgcat atacaagggt aggcagtcat ccttgtgtgg aggtgtacgg catggcggag
    26161 gaaaagggta cggcgggggc tcctctgggg gcggcggaag atcggaaggg gacgctggcg
    26221 attctcttgg gggcggcgtt tctcatggcg acgtcggcca tcgggccggg gtttctcacc
    26281 cagacggcgg tatttacgga aaagctaaag ggagcttttg cctttgccat tctggcgtcg
    26341 attctgattg acgtggtggt gcagctcaat atctggcgca tcctggcggt ttctggcctt
    26401 cgggcccagg atctggcgaa caaggtgctc cccgggctgg gatatttctt ggccttcgcc
    26461 gtgtcgttgg gggggctggt tttcaacatc ggcaacgtgg cgggggcggc catggggctc
    26521 aacgttttgg cgggggtgcc tctgattccc ggggcgctcc tctcggcggg gctggcggtg
    26581 gtgctctttt tgagcaagga aatgggacgg gccatggacc gttttgccca ggtcctcggg
    26641 tttgtcatga tcgccatggt gtgctacatg gtctttcgca ccaatcctcc cgtgggaacc
    26701 gcgctcaagg aaatggtgct gccttccacg gtggattgga tgatcgtcct gacgttggtg
    26761 gggggcacgg tgggggggta tatttctttt gcgggggccc atcgcatcat cgacgcaggg
    26821 atcaccggcc cccgggccct gggggacatc agccgagggg cggtgaatgc catcggcatc
    26881 acggggctca tgcgctttct cctctttttg gccatcctgg gcgtggtggt catggggact
    26941 cctctggatg cggccaatcc tccggcttcg gcgttccgca tcggcgccgg agagctggga
    27001 tatcgaatct ttggcgtcat cctgtgggcg gcggccatca cgtcggtggt aggggcttcc
    27061 tacacgtccg tctcgttctt gagaacgctt gccgctccca tcgagagcta tcatcgctgg
    27121 tggatcatcg ggttcatcgc ggtctccacg gcgatctttg tcacggtggg aaggccggtg
    27181 accctgctta tcttcgctgg cgccatcaat ggactcattc tgcccctggc cctcatttcg
    27241 gtcctgttgg gggcccaccg taaaaacatc gtgggagatt acaaacaccc gatgtggatg
    27301 tccgtggcgg ggtacgtcat ggcggccttc acgctctgga tggggttcac ttccctgggc
    27361 cctatcttca ccatgttcca aaagtagttc ggagggtata ccatggggaa cgggagacgc
    27421 gaacctcccg ttccattctt tttgaagggg gaaggactgt ggacggtccg acgaaacccc
    27481 gggtgctttt tgccggagat cgatgcgtgg tggtggagtt cggcacggcc atcgatcgcg
    27541 ccgtgaatgc ccgggtgcag cagttacggc gagatctaga ggcgagaccc ctggggggga
    27601 tcaccgagct ggtccccacc tatcgatcgc tggcggtgta ctttgatccc cgccgggtgg
    27661 gggaagggct gccggaactc ctggcaagcc gagcggcgag cctggagggt agtgcagaaa
    27721 cgggggggcg gtgtgtggtg atccccgtgg gatatggggg agagcggggc cccgatctgc
    27781 ccgaggtggc acgccacgcg gggctttcgg aaagtgaagt ggtctcccgc cacacggggc
    27841 gcgattatta ctgttacatg ctggggttca ctccggggtt tgcctacctg ggaggcatgg
    27901 atgaaacgct ggcgacgccc cgactggcca acccccgcac gaagatccct gcgggaagcg
    27961 ttgggatcgc cggggcccag acgggcattt atcccatcga cagccccggc gggtggcggc
    28021 tcatcggccg gacgccccta cggctcttcg atcccgacag gactcctccc acgctgctgg
    28081 atgcggggct ctgggtgcgc tttgtggcgg tggatgaggg ggagtacgcc gccgtgaccg
    28141 accaggtgcg ccgaggggcc tacgttcccg aggtccgaga cagggaggga aaccatgagg
    28201 cttgaggtgc ggcgtccggg gttgctctcc acggtgcagg acggggggcg ttgggggtac
    28261 cagggaaagg gcgttcccgt ggcgggggcc atggatctac ccgccttcag gcgaggaaac
    28321 ctcgtggcgg gaaacgagcc ggaagaggcg gcactggaga tcaccgtcct ggggcccacc
    28381 ctcggcgtcc ttgaaggaga agggatcgtg gcggcggcgg gggccgacct gggctttgcc
    28441 ctaaacgggg ctccggcgcc gcttttgacg gcggtgcggg tgcgctccgg ggacgagata
    28501 tcctttgcgg ggccccgggg agggggatgc cgggggtatc tctgcttcgc cggaggagtg
    28561 gcggtgcctc cggtgatggg gagtcgctcc acctttcttc gaggagccct ggggggcttt
    28621 catggacggg cgttgcgcgc gggcgacgtg ctggagaccg gcgacctccc ccccctcgcc
    28681 ttcgcggggg agggtttttc ctgccccccc gatctggatc tcgaccggtc ggcggatcgc
    28741 cccctgcggg tggtgccggg cccccaggat gatgccttca ccgccgaggg gcgggaaatt
    28801 ttcttttctt ccgcctatcg ggtcaccgac gaggcggacc gcatggggta tcggttggag
    28861 gggcctccgg tggcccattc tcgaggggcg gacatcgttt ccgatgccgt gcccctgggg
    28921 gccatccaga ttccggggca cgggcgcccc atctgcatgc tggcggatcg tcagaccacg
    28981 gggggctatc cgaaaattgc cgtgatctgt tccgacgacg tgggggtcct ggcccaaagg
    29041 atggcgggcg atacggtacg cttcgtggcg gtgacctttg ccgaggccct gcgatgggcc
    29101 aaggaagagg gcgatgcctt ggaaaatctg cgacgggccc ggagtacttg gcgatcgagg
    29161 ccccgattcc cggcgggatg ggatgcttcg ggaagatgga cccttacggt gggggaaagg
    29221 gcctacgcgg tgacgtggag ggcccggagt tgacgaaggg ggtgggggaa gccgtggcgg
    29281 tgatgatgat ggatctcaat agcgacctgg gagagagctt tggcgcctgg accatggggg
    29341 acgacgaggc ggtgctagct tcggtgagct cggccaacgt ggcctgcggt taccatgcgg
    29401 gggatcccga ggtcatgctg aagaccgtgc gggccgccgc ccggcggagc gtgggggtgg
    29461 gggcccatcc ggggtacccc gatttggtgg gcttcggtcg gcggaacatg gcttgctctc
    29521 cggacgaggt gtacgccttt acgctttacc agatcggggc tctgacggcg gtctgccggg
    29581 cctccggggt gtctctgcgg catgtgaagc cccacggggc cctttacaac caggcggcca
    29641 aggacccacg attggcggag gccatcgccc gggcggtgaa ggacggaggc gaggggctga
    29701 tgctgttggg gctggctaat tcggcctttg ggggagcggc ggcgacggtg ggggttccct
    29761 tcgcggcgga ggccttcgcc gaccgggcgt accgggccga cggcaccctg gtgccccggg
    29821 gggagccggg ggcggtgatc cacgacgagt ccgtggcggc ggctcgggtg gtgcgcatgg
    29881 ccaccgaagg ggtagtggag gccatcgacg gcacccttgt ttccctgcgc ccccattccg
    29941 tgtgcctcca cggcgacaca aaggaagcgg tgaccatggc acgggaggtt cgccgggcgt
    30001 tggaaggcgc gggcgtggag attagggctc tggaggtgcg gtgatgaaaa gcttttccga
    30061 tatgcaaggc ctttctccta gggagatccg ggagatcatc cgtcgggggg agtgggacgc
    30121 ccccacgccg gggctggctc cggggcatgt tcaggcgaat ctggtgatcc tcccccggga
    30181 gtgggcctat gattttctcg tcttcgcgat gcgtaatccc aagccctgtc ccgtactgga
    30241 cgtgacggag cccggagatc ccgaaccccg gaaaatggcc cccggagggg atctgcgcac
    30301 cgatctgccc cgatatcgag tgtgggaaca gggccagtgt gtggcggagc cccgggagat
    30361 cggggagtat tggcgggacg atctcgtggg ctttctcctg gggtgctcct tcagtttcga
    30421 gggagccttg acggaggccc gggtgccggt gcgccacatc gagtgcggat gcaacgtgcc
    30481 catgtacatc accaacgtcc cctgcgcccc ggcgggaagg ctctccggcc ctatggtggt
    30541 gagcatgcgt cccatccccg cctccctggt accccgggcg gtgctggcca cggggcgctt
    30601 tccggcggtg cacggagccc ccgttcacgt gggggatccc ggggccatcg gaatcggcga
    30661 tgtgcaacgc ccggacttcg gcgatccggt gccgatccgg gagggggaag taccggtctt
    30721 ctgggcctgc ggcgtgacgc cccaggcggc gctcatggcg tctcgccccc ccttcgccat
    30781 cacccacgcc ccggggcata tgttcctctg tgacccccgg gactcggatt tggcggtctt
    30841 ctagcttctt acggaaagga gcgccgtggc gattcccctt taccccggcg aggagtgatc
    30901 acgacggggg gatcgggccc aggcgggccc gcagggcggg ccagtggggg tcggggagcg
    30961 ccaggggagt cacggtccag gcgggatctt cgaggaaggc ggaggcgtgc tcgctgttgt
    31021 agtacacgat ctccgccccg tggggggcgt aacgaaggcg cacttcccga agaaagcccc
    31081 ccagggtgga ggcgtcgaag gggttaaaaa ggtaaaagag cgtggggaca ggggggagat
    31141 tggagaccag ccgcactgcg tccccggaga ggactcgcac ctgggggctg gagcggaagc
    31201 ggcggcgggt ccaggcggcg atcccccggt ttacctcgat gcccgtgagg tttttgtccg
    31261 cgaaaccatg ctggagccac cacgccagca cccgccccga tccgcacccc acgtccacca
    31321 gggcggaata gggggtctcg ggatcgggga agaggagtcc cagggccccc tcttccgtgg
    31381 ggcaggggcc gtgccctccg gggtgaaaac tggtgtaaca ggggcagagg acgtggcaga
    31441 ggagaaattc gcgaagcgac gtcatcgaca cggcgtggat cctcctttcg tgaccttgag
    31501 ggctatcata agttttcgga gagaaaaagt ccactaacct gaagcccccc gcgaacccct
    31561 ctttcggagg ggtataggcg ggagaataaa ggggccgttg cctagccaga ggccgggacg
    31621 ccccaaggga ctggggcgcc ccaggcggag ggctttccgg tctcaggacc cttcgtggcg
    31681 gcgcaggggg aggatggggg ccagctccac ctcggggaag tcgtccagct ccaccccggc
    31741 ggcggcctgg agaaaggtgc ccacccacca gaacacgtcc tggcgctgga tgcgcctcct
    31801 tagggagacc atgcgtcggc gctgctcctt ctcggacatt tcgaaggcct tccagatggc
    31861 ctccgccgta ccctcggtgt cgtaggggtt tatgaggatg gtgtcggaga gttgggcggc
    31921 ggccccggcg aactcgctca ggataagcac ccctcgccga tccacatgag aggcgcaata
    31981 ctccttgcag accaggttca tgccgtcctt taggggcgtc accagggcca tgtccgccgc
    32041 gagatagtgg gcgacgagct gggatcgatc catggattgg tagagataga ccacggggat
    32101 ccatccgtct ttggtgtact ttccgttgat gccgctgacg aggagctcta tgcgctcctt
    32161 gaggtcgcgg taggccttga ccgtctctcg gctgggcacc accacctgga cgagggatag
    32221 gcgtccccgg agctcggggt gccgttctag ggcgctttgg aaggccatga ggcgctctag
    32281 gatgcccttg gtgtaatcca ggcgatccac tcccagggcc agcttttcca tgcggtattg
    32341 ctcccggagg agggcggcct ggcgtttcac cggggcgctg cgggccgtgc ggtcaaagtc
    32401 gtcgtagtcg atactgatgg ggatggcggc ggcggaaaaa gatcgccctt ccaccgtgga
    32461 ggtcatgacg gggccccgtc cgtggacggt ggccgagggg aaaaaggccc gaagacaggt
    32521 gaggaagttt ctccggtccc gggcggtctg gaaggccacc aggtcgtagg cggtgagagc
    32581 ccgcaggagg gacatgcgcc agggaagctt ggcgaagata tccaggggag gaaaggggat
    32641 gtggaggaaa aagacgcatc gccgagacac gtcctggcga gatagcattt ccgccacgtg
    32701 gatcagatga tagtcgtgga tccagatcaa gttgcggggg gttgtgtgcc gggccacggc
    32761 ggaggcaaag agctcgttga ctcggaggta g
//
LOCUS       DAOOER010000018        32142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_1577_length_32142_cov_8.042852, whole genome shotgun sequence.
ACCESSION   DAOOER010000018 DAOOER010000000
VERSION     DAOOER010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 32142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32142
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1577_length_32142_cov_8.042852"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PL162_04045"
     CDS             complement(<1..487)
                     /locus_tag="PL162_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQF91222.1"
                     /translation="MWWRRFPLGALWTNGYLVSDGAGHGFFVDPGGDPAEVCGALAPS
                     DGVKISLERVLLTHGHADHIQGVDLLWECTGASVRISREDASMLDCPEKNLSSHMGTP
                     FYCRAPRELFCPGEVFAVGTMEVKVIATPGHTPGSCCFLVRDGEEEILFSGDTLFARS
                     VG"
     gene            complement(489..938)
                     /gene="dtd"
                     /locus_tag="PL162_04050"
     CDS             complement(489..938)
                     /gene="dtd"
                     /locus_tag="PL162_04050"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HQF91223.1"
                     /translation="MRSVVQRVSWARVSVEGQEVGSVGKGLCVLVGVTHEDGPKDVEW
                     MADKLIHLRIFEDEAGKMNKSLLDVGGAMMLVSQFTLLGDCRRGRRPSFLGAALPEKA
                     REVYDGLIAAVRSRGVSVASGVFQAEMAVELCNAGPVTLIIDSLGGA"
     gene            complement(1002..3287)
                     /locus_tag="PL162_04055"
     CDS             complement(1002..3287)
                     /locus_tag="PL162_04055"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HQF91224.1"
                     /translation="MVEAVGALGAEARGGAGAKDLRLLRDSFIGRIPEPERVSSLKLA
                     WQELWGKAATYLSREDLAMMGEALVTAAAAHGAQRRSSGDPYVVHPVQVASVLADMGM
                     DRDTLVAAMLHDVLEDTEILAAELASRFGTEVITLVDGVTKLGKLSFRSIEEYQAENL
                     RKMFLVMAKDIRVVLIKLADRLHNMRTIQALRPDKQLRIARETMDIYAPLAHRLGIYQ
                     IKRELEDLAFCVLDAEAYHDIRRRVRKKMPERENIVKKAIDVLQSELKAANIDVYITG
                     RAKHYYSIAEKMRRKNLAFDQLYDLLAVRVVVSSVVECYTALGMVHTLWKPLPGQFDD
                     YIANPKSNMYRSLHTTVVGPSGEPLEVQIRTWDMQWQAEYGIAAHWRYKGKRGAKASD
                     IDENLTWIRKALEEQGEGGEPREFLTHLKEDVLTSEVFVFTPKGDVISLPHGSTPIDF
                     AYNVHTEVGHKCVGAMVNGRIVSIDYALQNGDIVRVLTSPQGKPSRDWLKVARSNRAR
                     SKIRVYFRQQERAEREEYLSKGQEMLERELRRRVGDPPPVAPSEFGALLNRVARDMGY
                     AGAEELVVDLGMGHHSATGVIQKVLGFLHPVEGLDLPPPDLPAAPSPKREVDSEVVVE
                     GAEGVLVSLANCCRPVPGDAIVGLATKTRGITVHRQDCPSIEEMPPERRIEVAWGRRV
                     ATQYVSRVKIEASDRPGLFAEVSQAIQNADAAIMGLRANVRGASRAVMVAEIRVRNLE
                     HLYRVMGRVGSVAGVQEVARG"
     gene            complement(3347..4990)
                     /locus_tag="PL162_04060"
     CDS             complement(3347..4990)
                     /locus_tag="PL162_04060"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HQF91225.1"
                     /translation="MIATCTPEDLRVWRRDGASEALARRLGCGPLLASLLCMRGKDEA
                     LAGRELGVPPLEEALEGVQLGSSLPRESFLDRITPGKEVVVYGDYDVDGVTATALAVE
                     MCLSRGANVRYYIPHRHLKGYGLHKDMVDRIMQRGCDLLVVVDCGTHDRASHQAARDA
                     GVPTWIFDHHFAEGQEVPDAPVVLVNPQMGGNEEGRTLSAAGVLWVWAWQTGAMPRPW
                     LKNQLDLVALSTVADCAPLGAVNRMLVARGLDCLRSGHRRGIALLAERLDVRWRHTSE
                     EDLSMKIIPCLNAAGRLDLADVALRVLLEDKDQSAWMERLVALNRRRQLLSSQLVEEI
                     NPQVGASGCVLRGKGWPVGVLSSVASRLCSQHRKPVILSAQAGPILRGTVRMPPGGDA
                     VRFLSELSPHLLAWGGHRLAAGFSAAPESWEILEQAMTQELSSLRFSPECLDVLEVDP
                     ETVTPEAWREVGVLGPFGTGNPSPLFFCPFPSDSVPRPFGTQGAHVKIPRGRVDIVAF
                     YGTAFFGARPALRGLVYRPRLDHWRGSPRMQFVLERAVV"
     gene            complement(4987..5316)
                     /locus_tag="PL162_04065"
     CDS             complement(4987..5316)
                     /locus_tag="PL162_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91226.1"
                     /translation="MEALPSETLGVYAHPKVTEEDPAELCLECEGAGGEDLPAETPPL
                     EAVEIYAHPKVTEEDPAELCLECEGAEGGVSRAQGPSSDAWSEGDLSGGDDASAASRA
                     LGEVRKG"
     gene            complement(5265..5729)
                     /locus_tag="PL162_04070"
     CDS             complement(5265..5729)
                     /locus_tag="PL162_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HQF91227.1"
                     /translation="MKSYALAMAVAMLAAAGYVFQNPGDVTVRVLLWERQMPQGVWEV
                     LLFALGALLMWIVSLLALMEEVTPLRRKIAALEGAHRETKEEKDRLWVFWKASAPRAP
                     ELPPPRREISRRGQDSVGAGGKRIPPSPLRETAIYRWRPSPRKPWGSTPIRR"
     gene            complement(5932..6810)
                     /gene="secF"
                     /locus_tag="PL162_04075"
     CDS             complement(5932..6810)
                     /gene="secF"
                     /locus_tag="PL162_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HQF91228.1"
                     /translation="MALFRGIYIRFMEIRRVSLGISALMIAASLFFLATRGLNLGIDF
                     TGGLILQVEFSKATEVGRVRELLAQVGQSQATIQAYSDQGVFIRFSADDDATQKAVVQ
                     ALRGEDDAMKLLRMDKVGPVVGKQLREEALLALAVSLGGILLYVTLRFQFRFAVVSVL
                     ALVHDAIITLGVFSLLQREVGVSFIAAILTIVGYSLNDTIVVLDRVRENWGGLSKGIA
                     HLLNDSINQTLSRTINTSLTTLLAVVALLVWGGPVIQDFSLAMFVGILVGTYSSIYIA
                     GAMLCEWYERSPRVHA"
     gene            complement(6836..8227)
                     /gene="secD"
                     /locus_tag="PL162_04080"
     CDS             complement(6836..8227)
                     /gene="secD"
                     /locus_tag="PL162_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HQF91229.1"
                     /translation="MIRRDKWRLGLVGAIIVAGLVTVFPMEGRIRLGLDLRGGVHIVL
                     QAKGVDGAAVEDDSVERLLAVIRNRIDQYGVAEPVIQRSGKDRVLVDLPGVEDPQAAL
                     DLIGQTAQLEFRPVRSVSAKVPAAPQRKNYDDDSSFEAAQGRWQAAKDEVTASETKMK
                     EEAQGIPGALVAQDEAGTAYLLGPKAVSGNELQDAKTNYDNLGRAVVSLQFNSLGKDL
                     FDKATEENVGKQLAIVLDGQVISAPVVQERISGGQAQISGRFTPQDAQRLAIMLRAGA
                     LPVPVEILENRSVGPSLGSDSIQSGLKAGYIGAALVVLFMMIYYRLMGLAANIALAVA
                     MMLLFAGLISLKATLTLPGIAGIILTIGMAVDGNILIYERIREESRAGKTPMAALDAG
                     FRRAFTTILDSNLTTLIAAAALYYFGSGPIRGFAVTLSIGIVSSVFCAVVVTRVLLQA
                     MAGGSRGSGIIRS"
     gene            complement(8359..8700)
                     /gene="yajC"
                     /locus_tag="PL162_04085"
     CDS             complement(8359..8700)
                     /gene="yajC"
                     /locus_tag="PL162_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HQF91230.1"
                     /translation="MGQQGDLLGMLLPLVVFVVIFYFLLIRPQKKKQKQHETMLASIH
                     RGDDVVTAGGFFGTVREVKDDSFILEIADGVKARILKSSISYKRSAGEGKAADLSPEA
                     TGEQGLGDVKH"
     gene            complement(8722..9405)
                     /locus_tag="PL162_04090"
     CDS             complement(8722..9405)
                     /locus_tag="PL162_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HQF91231.1"
                     /translation="MAEPTVERLVCYRRLLSQLRDEGRRVVSSHDMGELLQIKASQVR
                     KDLSCFGEIGKRGVGYHVDRLLEHIQKILSSPHPWRMALVGVGNLGAALAGYKALRGE
                     TFSLEALFDVDPEKVGRTVAGVPCFSADAMEDVLREKDIQVVILAVPASAAQECADRA
                     GRSGTVRGILNFVPTSVVAPEGVMVFGVDISVALEKLLFYLKQEEAPGSGPLPSAMIE
                     SMGVRRSHS"
     gene            complement(9467..9847)
                     /locus_tag="PL162_04095"
     CDS             complement(9467..9847)
                     /locus_tag="PL162_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQF91232.1"
                     /translation="MKQCVVTDKAPAAVGPYSQAVWAGDFLYCSGQIPLDPATGALVG
                     STAREQAIQVLENVEGVLASQGLSMGHVVKATVFATDMKYFPEVNEVYAAKFTADPPA
                     RSFVQVAGLPKGALVEIEVVAWRG"
     gene            complement(9894..11177)
                     /gene="eno"
                     /locus_tag="PL162_04100"
     CDS             complement(9894..11177)
                     /gene="eno"
                     /locus_tag="PL162_04100"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HQF91233.1"
                     /translation="MAYIVGVHAREILDSRGNPTVEAEVWLDNGVVGQAGVPSGASTG
                     TFEALELRDGDRRYGGKGVLRAVENINDRIASALVGVDPADQAELDDIMIRLDGTPNK
                     GNLGANAILGVSMAAARAAATDHDLPLWAYLGGKGPFLLPTPLMNVLNGGAHADNNLD
                     IQEFMVVPHGAPSFPEALRMGTEVYHSLRKILKGKNYSVALGDEGGFAPRLAGNKEAL
                     ELLMEAIRGAGYAPGKDISLALDCAVTDLFRDGVYRFDGEGKTFTPEELVAYYADLCQ
                     TFPVVSIEDGMAEEDWDGWKLLTEALGKSVQIVGDDLFVTNPERLRQGMERGVANAVL
                     VKLNQIGSVTETLQVVDMARWNGYGAIISHRSGETDDTFISDLAVATGAGQIKTGAPA
                     RVDRVAKYNQLLRISEDLGEEAYAGSRRMKCARER"
     gene            complement(11213..11503)
                     /locus_tag="PL162_04105"
     CDS             complement(11213..11503)
                     /locus_tag="PL162_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HQF91234.1"
                     /translation="MRLDLYLKLVRLVKRRTLAQEMVTAGAVRLGGRRVKPAAEVKEG
                     DRVEVGYPRRLLVVEVLLADEAALRRGKEGYRVLEDRPLAPSTSPWGTSSEG"
     gene            complement(11581..12675)
                     /gene="rpoD"
                     /locus_tag="PL162_04110"
     CDS             complement(11581..12675)
                     /gene="rpoD"
                     /locus_tag="PL162_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HQF91235.1"
                     /translation="MGSKEDLLSYIDKVRGLLQEGQKKGYISYEDIEKYLPAEYLNNP
                     EALDNLHSNLLELGIEVQEDGKKGKLEDDDLIPVAAEDMGALEDLPLSDPVRMYLREI
                     GKVYLLTPEEEVDLAKRVEAGDPGAKNDLVAANLRLVVSIAKKYIGRGMLFLDLIQEG
                     NLGLIRAVEKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRV
                     SRQLVQRLGREPTAEEIAREMEIMPDKVEEIQRIAQEPVSLETPIGEEEDSQLGDFLE
                     DKDIPSPEDAAASQLLREQLEEMLDDLSDREREVLRLRFGLEDGHPYTLEEVGRRFGV
                     TRERIRQIEAKALRKMRHPSRSKKLRDFLE"
     assembly_gap    12688..12787
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13361..15100)
                     /gene="dnaG"
                     /locus_tag="PL162_04115"
     CDS             complement(13361..15100)
                     /gene="dnaG"
                     /locus_tag="PL162_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HQF91236.1"
                     /translation="MDMDVVRQIKERLDIVELVGDYVVLRRVGRQYQGLCPFHSEKTP
                     SFYVSPERQSYHCFGCGAGGDVFSFFMAQEGVDFAEARRLLAQRAGVSLPERSGKGPR
                     GGDALAVAQEFFLENLRGAGGEGPRNYLKRRQLSREAWDTFGLGWALPSWDALGKRLR
                     DRGVEPREAERAGLLVSGERGTYDRFRGRITFPIRDVSGRLVAFGGRLVDGEGAKYVN
                     SPEGELFRKRETLYLLSVAKRAIREKGRSVLVEGYMDALRLQGAGFPEAVASLGTALT
                     EEQAELLLRFAPRCFICYDSDSAGQEAALRGMYVLAHRGLDVRVVMLPEGKDPDDLLS
                     AEGGAVIFSRALENALPLVACHLALRRRHLEDPALRGTALKEIEAALAPLSPVEVAPH
                     VPLLAAAFRVDAHEVLRHLAESRQERGNPERGPSLRGRDRGEVPEKKEGELDPMEGAL
                     VYLLWTDGDLRRAASPEAVLPLLASEVARHVAAGLLQGDLPEELEDRWHRMGDLKPLA
                     LLAQGGAQCAALQGVDHPWQVVRDTLERRLWLREVMELKQRFRGNEADGTDMRRLTEL
                     ERRLKVKGGGTSA"
     gene            complement(15324..17288)
                     /locus_tag="PL162_04120"
     CDS             complement(15324..17288)
                     /locus_tag="PL162_04120"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HQF91237.1"
                     /translation="MGMEIMGITAGASILALAYAVFAAGRINAVEVGHEKVRELSSII
                     QSGAMAFLFREYRWLAPFVAVVGVLLFWQIGVASAVSFVFGALCSALSGFVGMKVATK
                     ANAKTAFSAIQGMNPALRVAFTGGTVMGMTVVGVGLLGIVVTYYLFPDPNIITGFGFG
                     ASSIALFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDIAGM
                     GADLFESYVNAVIAAMAVGLTVAGTKGIAYSLLLSALGILASLLGTFFVKVKEGGDPQ
                     LALRMGIISTGFFMIVGTYFLTINVFDGDVTLFYAVMGGVVSGVLIGYITEVYTSSSF
                     SSVKEIANASTTGAATNILSGIGVGMKSTAIPVLLICGATLLGYQFGGIFGIAVAAVG
                     MLATTGMTLSVDAYGPIADNAGGIAEMSHLPPEVRKITDKLDSVGNTTAAIGKGLAIG
                     SAALTALALFVAYAAAVKLNAIDLKDPHVMVGLFIGGMLPFLFSALAIQAVGRAAKAM
                     IDEVRRQFRTIPGIMEGTARPEYERCVDISTGAALREMVIPGLLAVLAPVVVGLGLGA
                     HALGGLLAGSIITGVMLAIFMANAGGAWDNAKKFIEEGNLGGKRSPAHSAAVTGDTVG
                     DPFKDTAGPSLNILIKLMSVVALVLAPLFS"
     gene            complement(17433..18350)
                     /locus_tag="PL162_04125"
     CDS             complement(17433..18350)
                     /locus_tag="PL162_04125"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HQF91238.1"
                     /translation="MDELSGERFGALDVGSNSVRTLVVERRDGVLRYAASSSEITRLT
                     QGWDAKRGRALLPEAVERTASEVREARRLLDRWGVSGDRRVCFATESLRDACHASEVL
                     KALEAAAGGPFRILSGSEEGHYAFLGARMALPEARGVFDLGGGSLEVCSEGKGESFPL
                     GAVRMTGQFAEDEGLLRSHVGAVLEGALWNDLSPLVGVGGTSSTVAMILGEVAVGSYR
                     PAAIHGRIISLEELRSLRRRLTGLSLEGRRRVRGMPEARADILPSGMMVIELLMEKTA
                     CPWYVHSECDLLWGVLGERAGKGRWKAVL"
     gene            complement(18343..18945)
                     /locus_tag="PL162_04130"
     CDS             complement(18343..18945)
                     /locus_tag="PL162_04130"
                     /inference="COORDINATES: protein motif:HMM:NF016314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HQF91239.1"
                     /translation="MTPENFHRWGLFRPFFGPWREGDGEALRLLGGEGFDGDLVLGIS
                     CRCAFGVPQVIGCAPLRRGRPFPTNWWLVCPFLREACAREEERGGVRSLEDHLKPWAK
                     EWREYARRHARWRLACLSPSTMRFLYRRRPGFWRALEGTGPGGIVPTETPRVKCLHLQ
                     LASWMVWPDHPGASWLAEKFPRLRCDAPQGCIAGRGENDG"
     gene            complement(19047..19460)
                     /locus_tag="PL162_04135"
     CDS             complement(19047..19460)
                     /locus_tag="PL162_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HQF91240.1"
                     /translation="MVEERETSVVPVKVGDDVTGTVEHIAPYGAFVRLTSTGQKAMIH
                     ISELSHSFIKKVEDVLELGQVITARVIKIDERGRIDLSIKALKPRESRPAPRSSPEDF
                     EKRLTSFLKVSDEKHAELNNKMKGKGGGKHRTTGG"
     gene            complement(19539..20207)
                     /locus_tag="PL162_04140"
     CDS             complement(19539..20207)
                     /locus_tag="PL162_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91241.1"
                     /translation="MGETLDRVREVLKRLFFGRAQVRGSFFPPERREFIHAMPRRALV
                     WHTLDREVRVLGARAYGKGSGRGGEVFGVPLGGSVPVHGEKVGPFSPSVVGVPLLVPS
                     EGVRRAAWETWNPWTVRRGSKTLLDRIPQRRRNGARFLRGRKLPVDGAVLAIFEAVVQ
                     KSVQRSVLDKGSGELLLWLRPEGGVPMGGLLVLVRRFQGSGQGLAWQWIKKDRGGGGF
                     SARS"
     gene            complement(20207..20740)
                     /locus_tag="PL162_04145"
     CDS             complement(20207..20740)
                     /locus_tag="PL162_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91242.1"
                     /translation="MGYLWVDDARDVFRGAALVTDFRGIPKDFRYSDPISPSRLERVL
                     YGGALEVYLREEIILTNLLAAVEFPPGLWICSARELLRPLRSLGGVPAVLLQESSHDP
                     LDFAGEVRPFGDETTFLVQLSELAAPLRLDVTREDLPRVGDLVALLGRAAETMDVKEP
                     FGRIVKALQAIDEEARG"
     gene            complement(20797..21123)
                     /locus_tag="PL162_04150"
     CDS             complement(20797..21123)
                     /locus_tag="PL162_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91243.1"
                     /translation="MIRPVEMQLSVWRAEQNATQQPRDPAAAAAQAAQQGEMAQESQA
                     RDQRVQEGQEAGAENRVTGRREESTGERRRRKRSPTGPSPEEIEEDPEEGSKASPSRK
                     GLDFYA"
     gene            complement(21155..22747)
                     /locus_tag="PL162_04155"
     CDS             complement(21155..22747)
                     /locus_tag="PL162_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HQF91244.1"
                     /translation="MRSNRPFRLVVKPEGVFLAVEGTAKEVVLAEVLAYIRSRDIQDY
                     DAEAVLRAVNEKPQDPVRIADRRDDLDRPAMIEVRPSDDGLKAELRITEPLGKPWPTV
                     EACKAEMGRKGVSFGIDEGALRKTIEDRRAGEWVGVARGVAPVDGKDAEFIFKVDLEP
                     ARPKAVDDSRVDMKDLGTVVNVIKGQELLEKKPAVISQDGTSVFGKTLKARLAKDKNI
                     PAGTNTVISDDGLHLFSVIDGCVKIKDNRVCVLPVFEVSGDVDYGTGNIQFVGDVVVK
                     GTIREGFEVSAGGGITVGGVVEGATVACEGDMVVRGGLRGMGKARVQVKGNLSATYVD
                     QAWVRCGRDLVVADVIMHSDVGARGTVNTITGKKGIIVGGKIQAGCEIRCEVLGNEMG
                     TRTEVVVGIPPEILEEKSQLQQNQKELEGKLGEIVSNVGYLKKIEALGRLDDQKREVL
                     LKLTRAQFQLQAQLGVIKDKLAALEVDMDRNRTEGRVSVHRSVYPGVAVTIRGVTYLV
                     RDKMDFISFMYIDGEVKPRPFQ"
     gene            complement(22884..23624)
                     /locus_tag="PL162_04160"
     CDS             complement(22884..23624)
                     /locus_tag="PL162_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HQF91245.1"
                     /translation="MIQDSERLMWQEYRDKRGASLRDTLVTRYLPLVRYVASRMGVTP
                     PPGLDFEDILSFGVFGLLDAMERFEPERGFSFQTFAVPRIRGAILDELRKYDWISRTG
                     REKLQRLERALEKFATAGRRVDDRALMAEMGVDEGAFREILEVAGRSYMASLDETVPF
                     DDGELDRSAMLADDAPLPSEVVEHREDVDRVMAMVRSLEEREQLVLSLYYGDGLTLKE
                     IGLVLGITESRVSQIHGKSIATLRALLR"
     gene            complement(23636..24622)
                     /locus_tag="PL162_04165"
     CDS             complement(23636..24622)
                     /locus_tag="PL162_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91246.1"
                     /translation="MEELMSLLHCTREEIEGAVATLGLEDAQARQVLGSLAPTHLAIK
                     GRFFSRKRGTAAGAFCLVAEGRSGTVRDRIIWVGGQDLPQSFSVDAGWEAVRSALLAV
                     ASTPDRILLRKIDQVIAPLTGPTELNILFSSPEKLDGFASRFREGLADAFHVDFMVEV
                     RAERFQRGRFEEIFPPEKKEGDPPPKDPLTEEDGASLQIYCAPVIDPVQGRPASLLKL
                     GDMVEVDFDRVAGFGRYVGRLCARTGRVPAFPISRIALLPSGDYLVRLDIADDVIGVM
                     KIPPPYRVRLHVPRRAPVFSIGRNDLRLLLEGGVLVAAMAVVAFAMVYFLLK"
     gene            complement(24771..25259)
                     /locus_tag="PL162_04170"
     CDS             complement(24771..25259)
                     /locus_tag="PL162_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HQF91247.1"
                     /translation="MAQTIHVGMADLVAVKVPSLLVTLGLGSCIGVVIYDPRNKVAGM
                     AHIMLPDSRDAGAVLKPGKFADTAVPRLLQELSALGALPSNLQAKIAGGAQMFNIPGK
                     DSGLLAVGARNIEATKRWLAHFNVPLVAEDTGGNKGRSIEFNVPLWNLSVKVLGVGVK
                     EL"
     gene            complement(25262..25882)
                     /locus_tag="PL162_04175"
     CDS             complement(25262..25882)
                     /locus_tag="PL162_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HQF91248.1"
                     /translation="MEWGDFSNLQLDAVREVGNIGTGNAATALSQLLGRAVDMGVPEA
                     DLIPIYELAGRYGSPETPVCAVLIRAEGDFPCNLIFVIDEGAGQELANLLIAMDISTL
                     DPELQIQMRDSALGEVGNIILGAFLNAISMLTGYVMPASVPAVAHDMLGSVMDIVASI
                     FGVIGDTALLVKTSLQVKDVECEVNGNVIMVPDPGSLETLLQKLGV"
     gene            complement(25897..28011)
                     /locus_tag="PL162_04180"
     CDS             complement(25897..28011)
                     /locus_tag="PL162_04180"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HQF91249.1"
                     /translation="MTVTGVDMNQYLGAFIDETSEQLQSLNELLLALESGSGDDGTIN
                     EIFRVAHTLKGMSATMGFEQMTHLTHAMEDMLDLVRKGAYTLEEEDISVLFSCLDRLT
                     AGLDLVRGGASDGALDIAPLVKTLHGRVKGGTKDMKEKPQSEAEVQIPFTDQERDWFA
                     EAREQGMGTYQLRVSLDPACALKAARAYMVVSRAGELGDVIKTIPAVEDLEKDPFQKP
                     FVVFLSSHETPEKIRDAVGRISEIRDVTLEAFFPALDGKASPERAKAPGGSSGAAEDL
                     SADASYETKIAPDNGEEPGGEEEPVPGPAVGGKGAKSQAVDAMKRTGRTVRVDIGRLD
                     KLMNLVGELVIGRARIERLAMEARLKAFEEPLSQLGRISGEIQELVTKLRMVPVSFVF
                     DKFPRLVRDLSKGMGKEVRLAIDGKETELDRNVIDEIGDPMVHLIRNSLDHGIEAPGD
                     RVKIGKPKEGTIHIAAYQEGNGVIIEVRDDGKGIDPAKIRAKAVEKGIMTREEAQGLT
                     DEEAIRLTFLPGFSTASVVTDLSGRGVGMDAVKSKVESLNGQFLVESRVGEGTRVLIR
                     LPLTLAIVLALLIRVGEETYAISLENVEETLLVRKEDIKYVHGTPVTNVRGEILSLRD
                     LGTVLGTSVERGDAGEYPVVVVRSGRSRLGFIVDELVGQQEIVIKPLGKLLAKVRGIA
                     GATILGDGNVALILDVASLHSR"
     gene            complement(28046..29149)
                     /locus_tag="PL162_04185"
     CDS             complement(28046..29149)
                     /locus_tag="PL162_04185"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HQF91250.1"
                     /translation="MRNAPIKGSPLRVLVVDDSAFMRRVISDILSRDPRLEVVGKARD
                     GEDALVQARALKPQVITLDVEMPRKNGLQVLEDIMRTQPTAVVMVSSLTAQGADVTMR
                     CLAAGAVDFVLKPSGTISLDMEKVAQELCQKVVLAGQVDLSHLAPQGGPKPLSDRVRP
                     VEAPGTVGRFLRLSPGTKPRVLVMAASTGGPRALQEILPALPARLSVPVVVVQHMPEG
                     FTASFAARMNDLSALAVVEGDDGLPLRPGLAVVARGGRHLVIEEGGKYGVCRLSDAPP
                     ENSVRPAADPLFRSAAKVFGGAVVGAILTGMGKDGTEGARVLRAQGATIISEAPETCV
                     VYGMPRVVFEAGLVDRQVPLYEMARALVNLVEE"
     gene            complement(29158..29832)
                     /locus_tag="PL162_04190"
     CDS             complement(29158..29832)
                     /locus_tag="PL162_04190"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HQF91251.1"
                     /translation="MELDGAKIESLLKPRVGERAEIRIDAGLHKGQFPSILESVEGLV
                     LGLGHPLRKGGLLPVYRDMALGVYLEDERLPLLVRGVALRSDLTAHVPLLWVRVVEDV
                     ERMQRRRYLRVTAVMPVQFWLMEQGESVHLSGRWREGTVLDLSLGGMRLQYRGSRATH
                     KDMLCLTLLPMGGKAFPVMGKIVWAPPQKEADLNEVGIEYVGVSRMLEKELGGFIRQQ
                     EMSGRT"
     gene            complement(29834..30805)
                     /locus_tag="PL162_04195"
     CDS             complement(29834..30805)
                     /locus_tag="PL162_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HQF91252.1"
                     /translation="MMETRESSPLAKGPIQEQILTPDPFSEDQAALLRSMVSAYAGGG
                     NPLRNRGIRTIAVVSGKGGVGKTNLSVNLALALGSQGWRVAVMDADLGLANVDILFGM
                     MPKYNLGHVIRGQRDLEDILVTVEERVMVIPGGAGVQELADLDQGRQAELIEKLAALE
                     GKVDILMVDTGAGIHRNIISFALASDTTVLLTSPEPTSIRDAYGVLKSLVMSSAGGLD
                     VRLLVNMAMSDEEAEAVAERIQMAANQFLGISVPYIGQIPWDKKVRDAVQERRPFFSS
                     FPGCRASLALLEVAGRLVGPENITDDGAGRGVKSFLFRLTRRLGLGR"
     gene            complement(30809..32113)
                     /gene="flhF"
                     /locus_tag="PL162_04200"
     CDS             complement(30809..32113)
                     /gene="flhF"
                     /locus_tag="PL162_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HQF91253.1"
                     /translation="MRVVRQITFEARNDADAVSMARDRLGRDAVILSTQVVKRGGFLG
                     FFRRKVLLVTAGILGDEAPRAAVAPEETKERIMAFQRLLEVKHAVRDAMGPKEAPSEV
                     RRESPPGVHLELSSQGKAMAAATAGASPLPAPLLSAPREPSPPAADEELKRQVDEIHQ
                     TLARVLSRLEEDRQEAVASAPSPWEELARRLEAADLSPHHAREVAEACGALGAEGVAP
                     YIASRIPVCGAEVRDALMGTRVMFIGPTGVGKTTTIAKLAAIYSLWERKKVLMLTADT
                     YRIAAVEQLRTYARILGVPFEVVFSPQEIPEVLAKHGDPDLVLLDTAGRSQRDARRME
                     ELQALYRVFRPDAVHLVVSANMKYRDILDVVDRMGVVPVSCLLFTKLDETLTYGNILN
                     AVLDFKKPLSFITTGQDVPNDIEVAEPDRIARLVAGEDGVGR"
BASE COUNT         5918 a        11384 c         8897 g         5843 t
ORIGIN      
        1 gccccacgct ccgggcaaac agggtgtccc cggaaaagag gatctcttcc tccccgtctc
       61 ggacgagaaa acagcaactc cccggggtgt gccccggagt ggcgatcacc ttcacctcca
      121 tggtccccac ggcaaagacc tcgccggggc aaaaaagctc ccggggagcc cggcagtaaa
      181 agggcgtgcc catgtgggac gaaaggtttt tttcagggca gtccaacatg gaggcgtctt
      241 ctcgactaat ccgtaccgag gccccggtgc actcccacag gaggtccacc ccctggatgt
      301 gatccgcatg gccatgggtg aggagcaccc gctccaggga tatctttact ccatccgaag
      361 gggccagggc tccgcacacc tctgcggggt ctcccccggg atccacgaag aagccgtgcc
      421 ccgctccatc gctcaccaga tacccattgg tccacagggc ccccaggggg aagcggcgcc
      481 accacatgct atgctccccc taggctgtct atgatcagcg tcaccggccc cgcgttgcag
      541 agctccacgg ccatttcggc ctgaaagacc cccgaagcca cagagacccc ccgggacctg
      601 acggcggcga tgagcccatc gtacacctcc cgggcctttt ccggcagggc cgcccccagg
      661 aacgaagggc gccgccctcg gcggcaatcc cctaagaggg taaactggga cacgagcatc
      721 atggctcccc ccacgtccag caaagattta ttcattttac ccgcctcgtc ttcgaagatg
      781 cggaggtgaa tgagcttgtc cgccatccat tccacatcct tggggccgtc ctcgtgggtc
      841 acgccaacca ggacacaaag gcccttcccg acgctcccca cctcttgccc ttccaccgaa
      901 acccgcgccc aggaaacgcg ctgcaccacg ctcctcaacg ctctcacccc ctggatctct
      961 cctggatttt cccccgacgc tcctctggag atgatcgaag gttatcctcg ggccacttcc
     1021 tggacccccg ccaccgatcc cacacgcccc atcacccggt agaggtgttc cagattacgc
     1081 acccggattt ccgccaccat caccgcccga ctggcccccc gcacgttagc ccggagcccc
     1141 atgatggcgg catcggcgtt ttgaatggcc tgggagacct ccgcaaagag ccccgggcgg
     1201 tcggaggctt ctattttcac tcggctgacg tactgggtcg ccacccggcg cccccaagcc
     1261 acttcaatac gacgctccgg gggcatctct tcgatgctgg ggcagtcctg gcggtgcacc
     1321 gtgatgcccc gggtcttcgt ggccaaaccc acgatggcgt ctcccggtac ggggcgacag
     1381 cagttcgcca gcgacaccaa gaccccttct gccccttcca ccaccacttc cgagtcgact
     1441 tcgcgcttgg gagagggggc ggcgggaagg tccggggggg gaaggtccag cccctccacg
     1501 gggtggagaa agcccagaac cttctgaatc acccccgtcg cgctgtgatg ccccatgccc
     1561 aggtccacca caagctcctc ggccccggca taccccatgt cccgggccac ccggttcagg
     1621 agggctccaa actccgacgg ggccacgggc gggggatccc ccacccggcg ccggagctcc
     1681 cgctccagca tttcttgtcc cttggagaga tactcttccc gctctgccct ctcctgttgg
     1741 cggaaataga cccggatctt ggaccgggcc cggttgctcc gggcgacctt gagccaatct
     1801 cgtgagggct ttccctgggg agaggtcaac acccgcacga tatcgccgtt ttgtagggcg
     1861 tagtctatgg agactatccg gccgttcacc atggctccca cgcacttgtg tcccacttcc
     1921 gtgtggacgt tataggcaaa gtctatgggg gtggagccgt gggggaggct gatgacgtct
     1981 cctttgggag tgaagacaaa gacctccgag gtcagcacgt cttctttgag atgcgtgaga
     2041 aactcccggg gttcgccgcc ctctccctgc tcttccaggg ccttgcggat ccacgtcagg
     2101 ttttcgtcaa tatcgctggc tttggcccct cgcttgccct tgtatcgcca gtgggccgca
     2161 atgccgtact ccgcctgcca ctgcatgtcc caggtgcgaa tctgcacctc gaggggttcg
     2221 ccgctggggc ccaccaccgt ggtgtgcaac gaacggtaca tgttgctctt ggggttggcg
     2281 atgtagtcgt cgaactgccc tggaaggggc ttccacaagg tatgtaccat gcccagggca
     2341 gtatagcatt ccaccacgct gctcaccacc acacgcaccg ccagcaggtc gtagagctga
     2401 tcgaaggcca aattcttgcg ccgcatcttc tcggcgatgc tgtagtagtg ctttgccctt
     2461 cccgtgatgt acacgtcgat gttagccgcc ttgagttcgc tctggagcac gtcgatggcc
     2521 ttcttgacga tgttctctcg ctcgggcatc ttctttcgca ctcggcgtcg gatgtcatgg
     2581 taagcttccg catctaggac gcaaaaggcc aaatcttcca gctcgcgttt gatctggtag
     2641 atccccagcc tatgggccag aggggcgtag atgtccatgg tctcccgggc gatgcgcagc
     2701 tgcttgtccg gacggagggc ctggatggtg cgcatgttgt ggaggcgatc cgctaacttt
     2761 atgagcacca cccggatgtc tttggccatg acgaggaaca tctttcgcag gttttccgcc
     2821 tgatattcct cgatggaacg aaaggagagc ttgcccagct tggtgacccc atccaccagg
     2881 gtgatgactt ctgtgccgaa acgggaggcc agctccgccg ccaggatctc cgtgtcctcc
     2941 agcacgtcgt ggagcatggc cgccaccagc gtgtcccgat ccattcccat gtccgccagc
     3001 acggaggcca cctgaacggg gtgcaccaca taggggtccc cggaagatct acgctgggcg
     3061 ccgtgggccg ccgccgccgt caccagagcc tcgcccatca tcgccagatc ctcgcgagat
     3121 aggtaggtgg ccgccttccc ccaaagttcc tgccaggcca gcttcaggct ggagacccgc
     3181 tcgggctcgg ggatccggcc gatgaaacta tccctcagga ggcgcaagtc cttggccccc
     3241 gcccctcccc gggcctcggc ccctagagcc cccaccgcct ccaccatggc ctggcccggg
     3301 atccgggagt gcctgtcatt agtcaacgag tcgttaggtt tcactgtcat accaccgcac
     3361 gttccagcac gaactgcatc cggggggatc ctcgccagtg atccagccga gggcggtaga
     3421 ccagcccccg aagggcggga cgggccccga agaacgcggt cccataaaag gccacgatgt
     3481 ccacccgacc ccgggggatc ttcacgtggg ccccctgggt cccgaaggga cgaggcacgc
     3541 tatccgaagg gaaggggcag aaaaaaagcg gcgaagggtt ccccgtgcca aaggggccca
     3601 gcactcccac ctcgcgccag gcctctggcg tcaccgtttc cggatcgacc tccagcacgt
     3661 caaggcattc gggggaaaag cgcagggaag agagttcctg ggtcatggct tgctccagga
     3721 tttcccagga ttcgggagcc gcgctgaacc ccgccgccaa gcgatgcccc ccccaagcca
     3781 gaaggtgggg gctgagctcg gagagaaagc gcaccgcatc gcccccgggg ggcattcgca
     3841 ccgtgccccg aaggatgggg cccgcttggg cggagagaat gacgggcttg cggtgctggg
     3901 agcacagtcg actcgccaca ctggaaagca cccccacggg ccatcccttg ccccggagga
     3961 cgcaccccga agcccccacc tggggattga tctcttccac caattggctc gaaaggagct
     4021 ggcgccgtcg gtttagggcc accagacgct ccatccacgc cgattggtcc ttgtcttcca
     4081 agagaacccg cagggccaca tcggccaggt ccaaacgccc cgccgcattg aggcagggaa
     4141 taattttcat ggaaagatct tcctcggagg tgtggcgcca ccgcacgtcc aaacgttccg
     4201 ccaagagggc aatccctcgg cgatgaccgg agcgcaggca atccaatcct cgggccacca
     4261 acatgcgatt cacggccccc aggggggcgc aatccgccac ggtggacagg gctaccaagt
     4321 ctagttgatt tttaagccaa ggccggggca tggccccggt ctgccaggcc cagacccaaa
     4381 gcaccccggc ggcgctcagt gtccgcccct cttcgttgcc ccccatctga gggttgacca
     4441 gcaccaccgg cgcgtcgggg acttcctgcc cctcggcgaa gtggtggtcg aagatccaag
     4501 tggggacccc cgcatcccgg gccgcttgat gggaagctcg gtcgtgggta ccgcagtcca
     4561 ccaccaccaa aaggtcgcat cctcgctgca taatgcgatc caccatgtcc ttgtggagcc
     4621 cgtaaccctt caggtggcgg tgggggatat agtaccgcac gttagccccc ctggaaaggc
     4681 acatctccac cgccaaggcc gtggcagtca ctccgtccac gtcgtaatca ccgtagacca
     4741 ccacttcctt gccgggggtg atacggtcca ggaaggactc tcgggggagg gatgatccca
     4801 gttggacccc ctccagcgcc tcttccaggg gcggcacccc caactcccgc cccgccaggg
     4861 cttcgtcttt accccgcata cacagaaggg acgcgagcag ggggccacac cccaagcggc
     4921 gagccagagc ctcggaagcc ccgtcccggc gccaaactct caaatcctcc ggggtacagg
     4981 tggcaatcaa cctttcctca cttctcccaa ggcacgggag gccgccgagg catcgtcgcc
     5041 ccccgaaaga tccccttcgc tccatgcatc ggaagagggc ccttgagccc tggaaacgcc
     5101 cccttccgcc ccttcgcact ccaggcagag ctcggccggg tcttcttctg tcaccttcgg
     5161 atgggcgtag atttccacgg cctccagggg aggggtctcc gccgggagat cctcgccccc
     5221 cgctccctcg cactccaggc agagctcggc cgggtcttct tccgtcacct tcggatgggc
     5281 gtagaccccc aaggtttccg aggggagggc ctccaccggt agatcgccgt ctcccgaagg
     5341 gggctcggcg ggatcctctt ccccccggcc cccaccgagt cctgacccct tctcgaaatc
     5401 tcccgacgag ggggaggaag ctctggagct cgaggggcgg aggctttcca gaacacccac
     5461 aggcggtcct tttcttcctt cgtctctcgg tgtgcccctt caagggcagc gatctttcgt
     5521 cgaagggggg tcacttcctc catgagggcc aaaagggaga cgatccacat gagcagggct
     5581 cccagggcaa agagcaacac ctcccacacc ccttggggca tctgccgctc ccagaggagg
     5641 acgcgcaccg tcacatcccc cggattttga aagacgtacc ccgccgccgc cagcatggcc
     5701 accgccatcg ccagagcgta gcttttcatg ggaaccactc cttccttcca cgccttgatt
     5761 gatccccttt tttacctacc ccgggaatct tgggcccctc ctcaggggat cccggcctcg
     5821 cccatcgccc tccgtaccga cgaagggagc cgagcagggg cccgaggccc gccgggggtc
     5881 cgaagacgcc cctatctccc cggcgccccg gatctctcgg gccatctcaa atcacgcgtg
     5941 aaccctgggc gacctctcat accactcgca cagcatggcg ccagcgatgt agatggagct
     6001 gtaagtcccc accaaaatcc ccacgaacat ggccagggag aagtcctgga tcacggggcc
     6061 tccccacacc agaagcgcca ccaccgccag cagggtggta agggaggtgt tgatggtgcg
     6121 ggaaagggtc tggttgatgg agtcgttgag tagatgggcg atgcccttgg agagccctcc
     6181 ccagttttcc cgcactcggt ccaacaccac aatggtgtcg ttcagagagt accccacgat
     6241 ggtcaggatg gcggcgatga aagatacccc cacctctcgt tgcaggaggc tgaagacccc
     6301 cagggtgatg atggcgtcgt gaacgagggc caggacgctc accacggcga agcggaattg
     6361 aaagcgtagc gtgacataga gaaggatccc ccccaaagac accgccaggg ccaggagggc
     6421 ctcctcccgg agctgcttgc ccaccacggg gcccacctta tccatgcgga gcagcttcat
     6481 ggcgtcgtct tctccccgca gggcctggac cacggccttc tgggtggcat catcgtcggc
     6541 ggagaagcga atgaacactc cctggtcgct gtaggcctgg atggtggctt ggctctgacc
     6601 gacctgggcc aaaagctccc tcacccgtcc cacttcagtg gccttgctga actccacctg
     6661 aaggatgagc ccccccgtga agtcgatgcc caagttcaac ccccgggtcg ccagaaaaaa
     6721 caagctggcg gcgatcatga gggcgctgat ccccaggctc acgcgccgga tttccataaa
     6781 ccggatatag attcctcgaa acaaagccat cccttcgttc ctccctactt ccgagtcagc
     6841 tgcggatgat cccggatccc cgggatcccc cggccatggc ctggagcaat acccgagtga
     6901 ccaccacggc gcagaagacg ctggacacaa taccgatgga aagcgtcacc gcaaaacccc
     6961 gaatcgggcc gctaccgaag tagtacaggg ccgctgcggc gatgagcgtg gtaaggttag
     7021 agtccaaaat ggtggtgaag gcccgtcgga accccgcatc cagggccgcc atgggggtct
     7081 tccccgcccg ggattcttcc cggatgcgct cgtagatcag gatgttgccg tccaccgcca
     7141 tgccgatggt gaggatgatg cccgcgatgc cgggaagggt cagggtggct ttcagcgata
     7201 tgagcccggc gaagaggagc atcatggcca ccgccagagc gatgttagcg gccagcccca
     7261 tgaggcgata ataaatcatc atgaatagca ccaccaaggc cgccccgatg taccccgcct
     7321 tgagccccga ttggatggag tccgacccca aggagggccc caccgaccgg ttttccagga
     7381 tctccacggg caccggcaac gccccggctc gcagcatgat ggccaagcgc tgggcgtcct
     7441 ggggggtgaa gcgccccgaa atctgggctt ggccgccgct tatgcgctcc tggaccacgg
     7501 gggcagagat cacctggccg tcgaggacga tggccaattg cttacccaca ttttcctccg
     7561 tggccttgtc aaaaaggtcc ttccccaggg agttgaactg aagggaaacc accgcccgcc
     7621 ccaggttgtc gtagtttgtc ttggcatcct ggagctcgtt gcccgacacc gccttgggcc
     7681 ccaaaaggta ggccgtccct gcctcgtcct gggccacaag ggctcccggg atgccctggg
     7741 cttcctcttt catcttcgtc tcagaggccg tcacttcgtc tttggccgcc tgccaacgcc
     7801 cctgggccgc ctcgaaggag ctgtcgtcgt cgtagttttt ccgttgcggc gcagcgggca
     7861 ccttggcgga aacggatcgc accgggcgga actccaactg ggccgtctga ccgatgaggt
     7921 ccaacgccgc ctggggatct tccaccccag gaaggtccac cagcactcgg tccttccccg
     7981 agcgctgaat cacgggctcc gctacgccat actggtcgat gcggtttcgg atcaccgcca
     8041 acaggcgttc cacgctgtca tcctccacgg cggccccatc gacccctttg gcctggagga
     8101 cgatgtgcac cccgccccgg agatcgagcc ccagccggat cctaccctcc atgggaaaga
     8161 ccgttacgag tcccgcgacg attatcgctc ccaccaaccc caggcgccac ttatcacgtc
     8221 gaatcatgcc cacatcctcc cattcctccg cttcggttta caaaaaaatt cgttaccccg
     8281 gatcaccgag agccgcacga caaaaacgga gcaggcaaag gcgcccgccc cgctcctgga
     8341 caccgggaag aatcctcctt aatgtttcac gtctcccaag ccctgctccc ccgtcgcctc
     8401 cggcgatagg tccgcagcct tgccctctcc cgcagaacgc ttgtaggaga tggagctctt
     8461 caggatccgg gccttcaccc cgtcggcgat ctccaagata aagctgtcat ctttaacctc
     8521 tcggaccgtg ccgaaaaagc cccccgccgt gaccacatcg tcccctcgat ggatggacgc
     8581 caacatggtt tcatgttgct tttgcttttt cttctgaggc cttatgagta aaaagtaaaa
     8641 aatcaccaca aacactacca gggggagcaa catccccagc agatctccct gctgacccat
     8701 aaaccacacg cctcccttaa gtcaagaatg gctcctacgt actcccatgg attctatcat
     8761 ggcggaagga agagggccac tgcccggggc ctcttcctgt ttgagataga acaagagctt
     8821 ttccagcgcc acggaaatgt ccacgccgaa aaccatcacg ccctcggggg ccaccacgga
     8881 cgtcggaaca aagttcaaga tcccccgcac ggtgccgcta cgccccgccc gatcggcgca
     8941 ctcctgggcc gccgatgcgg gcaccgccaa aatcaccacc tgaatgtctt tctcgcgcaa
     9001 gacgtcttcc atggcgtccg ccgaaaaaca cggcaccccc gccacggtcc gccccacctt
     9061 ctccgggtcg acgtcaaaaa gggcctccaa agagaacgtc tccccccgga gcgccttgta
     9121 ccccgccaac gccgccccga ggtttcccac ccccaccagg gccatgcgcc acggatgcgg
     9181 agaggacaag atcttttgga tgtgctccag aaggcgatcg acatgataac ccacgcccct
     9241 tttgccgatc tccccgaaac aggacaggtc ctttcgaact tggctcgcct tgatctggag
     9301 gagttctccc atgtcgtggg atgacaccac ccgccgtccc tcgtcccgca gttggctcag
     9361 cagacggcga tagcacacga ggcgctccac ggtgggctct gccacctttc ttcccccctg
     9421 ggaaaaaagc ccctcgaaaa ggtcttcctc gcccactccg ccgcctctat ccgcgccaag
     9481 ccacgacttc gatttccacc aaggcgccct tcggaagccc cgccacctgg acgaaggatc
     9541 gggccggggg atcggcggtg aacttggcgg cgtaaacctc gttaacctcg ggaaagtact
     9601 tcatgtcggt ggcaaagacc gtggccttca ccacgtgccc catggaaagc ccctgggaag
     9661 cgagcacccc ctccacgttc tccagcacct ggatcgcctg ctcccgtgcg gtggatccca
     9721 ccagggcccc cgtggccggg tcaaggggga tttgccccga acagtagagg aagtcccccg
     9781 cccacaccgc ctggctgtaa ggtcccaccg cagcgggggc cttgtccgtc accacacact
     9841 gtttcatgtc ctatcgcctc cctttttgtt cccttcgcct ccctttttgt tccctatctc
     9901 tcccgggcgc acttcatgcg ccgggatccc gcataggcct cttcgccgag gtcttccgat
     9961 atgcgaagga gttgattgta cttggccacc cgatccaccc gggcgggggc ccccgtcttg
    10021 atctgccccg cgccggtggc cacggccaag tcgctgatga aggtgtcgtc cgtctccccc
    10081 gatcggtgag agatgatggc accataaccg ttccaccggg ccatatccac cacctggagc
    10141 gtctccgtca ctgacccgat ctggttaagt ttgaccagca cggcgttggc cactcctcgc
    10201 tccatgccct gacggaggcg ttcgggattc gtgacaaaga ggtcgtctcc cacgatctga
    10261 acggatttcc ccagggcctc cgtgaggagc ttccagccgt cccaatcctc ctccgccatg
    10321 ccatcctcga tggataccac ggggaaggtc tggcaaaggt cggcgtagta cgccacgagc
    10381 tcctcggggg tgaaggtctt cccctctcca tcgaagcggt agaccccatc tcggaaaagg
    10441 tccgtcacgg cgcagtccag agccagggat atgtctttcc ccggtgcgta ccccgccccc
    10501 cggatggctt ccatcaagag ctccagggcc tccttattgc ccgccaggcg gggggcaaag
    10561 cctccctcgt cccccagggc cacgctgtaa tttttcccct tcaggatctt ccggagcgag
    10621 tggtaaactt cggtgcccat gcgcagggcc tcggggaagg agggggcccc gtgggggacg
    10681 accatgaatt cctgaatatc caggttgttg tccgcatggg cccccccatt taggacgttc
    10741 atcaagggag tggggaggag aaagggcccc ttccctccca ggtaggccca gaggggcagg
    10801 tcgtgatcgg tggcggcggc ccgggcagcg gccatggaca cccccaggat ggcattggcc
    10861 cccaggttgc ccttgttcgg agtcccgtcc aagcggatca tgatatcgtc gagctctgcc
    10921 tgatctgcgg gatccactcc caccagggcg gaggcgatgc ggtcgttgat gttttccacc
    10981 gcccggagga cgcccttgcc cccgtatcgc ctatccccat ctcggagctc cagggcttca
    11041 aaggtccccg tcgatgctcc cgaagggacc cccgcctgcc ccaccacgcc attatctagc
    11101 caaacttcgg cctccaccgt ggggtttccc cgggagtcca ggatctcccg ggcgtgcaca
    11161 ccaactatgt acgccatgac aaaaccacct ttccttcgcg ctctcctcag ggctagccct
    11221 cggatgaggt cccccagggg gaggtggaag gagctaacgg acgatcttcc agcacgcggt
    11281 atccctcttt cccccgtcgc agggcagctt catccgccag aaggacctcc accaccagaa
    11341 ggcgccgagg ataacccacc tccacccgat ccccttcttt cacctccgcc gcaggcttca
    11401 ccctgcgccc tcccaggcgt accgcccccg ccgttaccat ctcttgggcc aacgtccgcc
    11461 gcttcaccag tcgcaccagc ttgagataga gatccagcct catccgtcct cacgtcccta
    11521 agtataccca tcgtccccgt tccatacaac ggagacgttc tttttttaac cttatatctc
    11581 ctattcgagg aagtcccgca gctttttgct ccgactggga tggcgcattt tcctcagggc
    11641 cttggcctcg atctggcgaa tgcgctcccg ggtgacgccg aaacgcctcc ccacttcttc
    11701 cagcgtgtag ggatggccat cctccaggcc aaagcggagg cgcagcacct cccgctcccg
    11761 atccgacaag tcgtccaaca tctcttcgag ctgctcccgc aaaagttggc tcgccgccgc
    11821 atcttcgggg ctcgggatgt ctttgtcctc caaaaagtcc cccaactggc tgtcttcttc
    11881 ctcgccaatg ggagtttcca gggatacggg ttcctgggcg atgcgctgga tctcttctac
    11941 cttgtccggc atgatctcca tctcccgggc aatttcctcc gctgtgggct ctcgccccag
    12001 acgttggacc aactggcgcg agaccctaat gagcttattt atggtctcca ccatgtggac
    12061 ggggatgcgg atggtgcgag cctggtctgc aatggcccgg gtgatggcct gacggatcca
    12121 ccaggtggca taggtgctga acttgtaccc cttgcggtag tcgaacttct ccaccgcccg
    12181 gatgagcccc aagttcccct cctggatgag gtccagaaag agcatccccc ttccgatgta
    12241 ctttttggcg atgctcacca ccaggcgcag gttcgccgcc accagatcat ttttggcccc
    12301 ggggtctccg gcctccaccc gtttggccag gtccacttct tcttccgggg tcaaaagata
    12361 gaccttgccg atctcccgga gatacatgcg gacgggatcc gacaggggaa ggtcttccaa
    12421 agctcccatg tcctccgccg ccacagggat gagatcgtcg tcttccaact tgcctttttt
    12481 cccgtcctcc tgaacctcaa tgccaagctc cagcaggttc gaatgcaggt tatccagggc
    12541 ctcggggttg ttcagatact ccgccggaag gtatttttca atgtcttcgt aagaaatata
    12601 tccctttttt tgcccttctt gtaggagacc tcgcaccttg tcaatgtagg ataaaaggtc
    12661 ttctttggac cccaggtcgt ctccggcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12781 nnnnnnnccc cttttttgcg cctcgtcggc ttctttttcg aatccttcgc cgaagggcct
    12841 ccttccggag cctccgtcga aagggggcgt ttcagggcct ctaccgaaga ctttacctct
    12901 ggaaaggact ccgcgacgac cgccgggggg acttccggga atccctcccc ccgatcgccc
    12961 atcgggaggc cttccggggc ccggggcacc tcgggagctt ccgagatccc ttcccctttt
    13021 ttgcgcctcg tcggcttctt tttcgaatcc ttcgccgaag ggcctccccc tgggacgtca
    13081 accggggcct tctcttccgg agcctcggcc gacgcttttc ccgtcgtggc cccacctttt
    13141 gtcttctttt tggcaaactt gggggagtcc tttggctcgg atgccgcacg ccggggttct
    13201 tccggagccg gggagggcgg gacgggctcc ggccctactc ctttcgccac tggcgccagg
    13261 gaccgagggg ccttcgttgc cccggcggcc ctacgaagcg gcgcgtcatg aagcggcgtc
    13321 ccctccgccg ccaatggggc ctcttttcct ctaggttttt tcacgctgaa gttccccccc
    13381 ctttcacttt gagccgacgc tccaattccg taagccggcg catgtcggtg ccatccgcct
    13441 cgttgccccg gaagcgttgc ttcagttcca tgacctcccg gagccacagg cgccgctcca
    13501 gggtgtctcg taccacctgc caaggatggt ccaccccttg gagggcggca cactgagccc
    13561 ctccctgggc cagtaaagcc aagggcttta ggtcccccat cctgtgccac cgatcctcca
    13621 actcctccgg caagtccccc tggagaagcc ccgccgccac gtggcgggcc acctccgagg
    13681 ccagcagggg aaggaccgcc tccgggctcg ccgctcggcg aagatctccg tcggtccaca
    13741 acaaatatac cagcgcccct tccatgggat cgagctcccc ttcttttttc tcgggaactt
    13801 ccccccggtc tcgcccccgt agggagggcc ccctttcggg gtttccccgt tcttggcggc
    13861 tctccgccag gtgccgcaag acttcatggg catccacgcg aaacgccgcc gccagcagag
    13921 ggacatgggg agccacctcc accggagaga gaggagccag ggccgcctcg atttccttga
    13981 gggccgtccc tcgcagggcg gggtcctcca gatgccttcg gcgcaacgcc aggtgacacg
    14041 ccaccagcgg cagggcgttt tccaaggccc gggaaaagat caccgcacct ccttccgccg
    14101 acaggaggtc atcgggatcc ttcccctcgg gaagcatcac cacccgcacg tccaaccccc
    14161 ggtgcgccag gacgtacatc ccccgtagcg ccgcctcttg ccccgcgctg tcgctatcgt
    14221 agcagatgaa acatcgggga gcgaagcgga gcagaagctc ggcctgttcc tccgtgagcg
    14281 ccgtgcctag ggaggccacg gcctcaggga agcccgcccc ctggaggcgc agtgcgtcca
    14341 tgtagccctc caccaggacg gaacgtccct tttctcgtat ggcacgcttc gctacggaaa
    14401 gcaaatagag cgtctctctc ttgcggaaaa gctctccctc ggggctgttg acgtacttgg
    14461 ccccttctcc gtccaccagg cgtcccccga aggccacgag gcgccccgat acatcgcgga
    14521 tgggaaaggt gatccgccct cgaaatcggt cgtaggtccc ccgctccccc gacaccagaa
    14581 gtcccgcccg ttccgcctct cgcggctcca cccctcgatc ccgcagcctc ttccccaggg
    14641 catcccaaga cggaagggcc catcccaaac caaaggtgtc ccaggcttct cgggaaagtt
    14701 ggcgccgttt caggtagttt cgcggtccct ccccccccgc tccccggagg ttttccagga
    14761 aaaactcctg ggccaccgcc agggcgtccc ctccccgggg ccctttcccg gagcgctccg
    14821 gaagggacac ccccgctcgc tgggccaaga gccgtcgggc ctcggcaaaa tccaccccct
    14881 cctgggccat gaagaaagaa aaaacgtctc ctccggcgcc gcaaccgaaa caatggtagc
    14941 tctgccgctc cggggacacg taaaaagagg gggttttctc gctgtggaag gggcaaagac
    15001 cttgatactg ccgccccacc ctccgaagca ccacgtaatc ccccaccaac tccacgatgt
    15061 caagccgctc cttgatctgc cggacgacat ccatatccat gggggatctt cgctcctctc
    15121 gtcgctccta ggccacccat cttgagggaa aaggccagca cagaacgggg gaaaggtccg
    15181 caccccttcc cccgttcctc gcttcgccgc acgcgccccc aggtgcatca cgacgggcct
    15241 gaggtttcac cgttccggaa gacctttccg tcccgttccc ttttcaggag gggcggattg
    15301 ctccggggtt tttgctttat tctttaagag aacagcgggg ccaacaccag ggccaccacg
    15361 gacatcagtt tgatgaggat attcaggcta gggcctgcgg tgtccttgaa ggggtctccc
    15421 acggtgtctc ccgtcacggc ggcggagtgg gccggggatc gtttaccgcc aaggtttcct
    15481 tcttcgatga actttttcgc attatcccag gcgccccccg cattggccat gaagatggcc
    15541 aacatgaccc ccgtgatgat ggatcccgcc aggagccccc ctagggcgtg agcccccagg
    15601 cccaaaccca ccaccacggg ggccagcacg gccaacaggc cggggattac catctctcgc
    15661 agggccgccc cggtggagat gtccacacat cgctcgtact ccggccgggc cgtgccctcc
    15721 atgatgcccg gaatggtccg gaactggcgc cgcacctcgt cgatcatggc cttggcggcc
    15781 cgccccacgg cctgaatggc caaggcggag aacaggaagg gaagcatacc gccgatgaag
    15841 agccccacca tgacgtgggg atctttgagg tcgatggcgt tgagcttcac tgcggcggcg
    15901 taggccacaa aaagcgctag ggccgtgagg gccgccgatc cgatggccag ccccttgccg
    15961 atggcggcgg tggtgttgcc cacggaatcc agcttgtcgg tgatcttgcg cacctccggc
    16021 ggcaggtggc tcatctcggc gatgcccccg gcgttgtccg cgatgggacc ataggcgtcc
    16081 acggagaggg tcatccccgt ggtggcaagc atccccacgg ccgccacggc gatgccgaag
    16141 atgccgccaa actggtatcc cagaagggtg gcgccgcaga tgagcaacac ggggatcgcc
    16201 gtggacttca tgcccacccc gatgcccgac agtatattcg tggcggcccc ggtggtggat
    16261 gcgttggcaa tttctttcac cgaagagaag ctcgaggagg tatagacctc ggtgatgtac
    16321 ccgatgagca ctccggacac gaccccgccc atgacggcgt aaaatagggt cacgtcgccg
    16381 tcaaagacgt ttatggtaag gaaataggtc cccacgatca tgaaaaatcc cgtgctaatg
    16441 atgcccattc gcagggccag ctgagggtct cctccctctt tcaccttgac gaagaacgtc
    16501 cccagcaacg aggccaggat gcccagggcg gaaaggagaa gagaataggc gatgcccttg
    16561 gtccccgcca cggtgagccc caccgccatg gcggcgatga cggcgttgac gtaggattcg
    16621 aagagatccg cccccatgcc cgcgatgtct cccacgttgt ctcccacgtt gtccgcgatg
    16681 accgccgggt tccgggggtc atcttcggga atccccgcct ccaccttgcc caccaggtcc
    16741 gcccccacgt ccgccgcctt ggtgtaaatc ccgccgccca cgcgggcgaa gagggcgatg
    16801 gagctggcgc cgaagccgaa gcccgtgatg atgttggggt cagggaagag gtagtaggtc
    16861 accacgatgc ccaggagtcc cacccccacc accgtcatgc ccatgacggt gccaccggtg
    16921 aaggccaccc gcaaggcggg gttcatgcct tggatggcgg aaaaagccgt cttcgcgttg
    16981 gccttggtgg ccactttcat gcccacgaac cccgaaagag cgctgcaaag ggccccgaac
    17041 acaaaagaca ccgccgaggc caccccaatt tgccagaaaa gcaacactcc caccacggcc
    17101 acgaagggag ccagccagcg gtattcccgg aacaaaaagg ccatggcacc gctctgaatg
    17161 atggacgaca gctccctcac cttttcgtgc cccacttcca ccgcattgat acgtcctgcg
    17221 gcgaataccg cgtacgccaa ggcgagaatg ctggcccccg ctgtgatgcc catgatctcc
    17281 atacccatcc gcacacaacc tcctcaggca aaaagccgaa atagggcgcc cacgcccccc
    17341 gaaccccttt cattataggc gcttcagacc tttgagacaa cggggcaacg gttctcctgg
    17401 ggcagcgccc cagtccttaa ggggaaaaaa ggctacaaaa ctgccttcca cctccccttt
    17461 cccgcccgtt cccccaaaac cccccagagg aggtcgcatt cgctgtggac gtaccaaggg
    17521 caagcggttt tttccatcag cagctctatc accatcatcc ccgacggtag gatatccgcc
    17581 cgggcttcgg gcattccccg aacccggcgc cgaccctcca gggacagccc cgtcaggcgc
    17641 cttcgcaacg atcgcagctc ttccagggaa attatccggc catgtatcgc cgcagggcga
    17701 taggatccca ccgctacttc ccccaggatc atcgccaccg tggaggaagt tccccccact
    17761 cccaccaggg gtgacaggtc attccacagg gccccctcca acacggcccc cacgtgggac
    17821 cgcagaagcc cttcgtcttc ggcgaattgc cccgtcatgc gcacggcccc caggggaaaa
    17881 gactctccct tcccctccga gcacacctcc aggctgcctc cccccaggtc gaacacccct
    17941 cgggcctcgg gaagggccat gcgggccccc aggaaagcgt agtgtccctc ctcgctcccg
    18001 gagaggatcc taaaagggcc tccggcggcg gcctccaggg ccttcagcac ctcggaggca
    18061 tgacaggcgt ctctaaggga ttccgtggca aaacagaccc ttcggtcccc ggaaaccccc
    18121 caccgatcca atagtcgtcg ggcctcccgc acctccgacg ccgtgcgctc gacggcctca
    18181 gggagcaacg ccctccccct ctttgcatcc cacccctggg tcaggcgcgt gatctctgat
    18241 gacgaggccg cgtaccgtaa aactccgtcc cggcgctcca ccaccagcgt ccgtacggag
    18301 ttagacccca catcaagggc cccgaacctc tccccggaaa gctcatccat cgttttcgcc
    18361 cctccccgcg atacatccct gcggagcatc gcatcggagc cggggaaatt tttccgccaa
    18421 ccaggaggct ccggggtggt cgggccagac catccaactg gccaactgga ggtggaggca
    18481 cttcaccctc ggcgtctcgg tggggacgat cccccccggc cccgtgccct caagggcccg
    18541 ccagaagccc gggcgacggc gatacaaaaa ccgcatcgtc gaagggctta agcacgccaa
    18601 gcgccaccgg gcgtgacgcc gggcgtactc cctccattcc ttagcccagg gcttgaggtg
    18661 gtcttccaga ctccgcaccc ccccccgttc ttcctcccgg gcacaggcct cccgcagaaa
    18721 ggggcatacc aaccaccaat tggtgggaaa gggtcttccc cggcgcagag gggcgcaccc
    18781 tatcacctgc ggcacgccga aggcacagcg acaggagatc cctaagacga gatccccatc
    18841 gaaaccctct cctcccagaa ggcgaagggc ctccccgtct ccttccctcc aggggccaaa
    18901 aaaaggccgg aagagccccc agcggtgaaa gttctccgga gtcatcgcgc caccttcggg
    18961 ccctcgccct ggcaaaaaag accccgcccc cgccagtgcc cccccccccc aaactccgac
    19021 gatcagaggg ctatggccag accttttcat cccccagtcg tgcgatgctt cccgccaccc
    19081 tttcccttca tcttattgtt aagctctgca tgtttttcgt cgctcacttt aaggaaagag
    19141 gtgagtcgtt tttcgaagtc ctccggcgaa gagcgcggcg ccgggcggct ttcccgaggt
    19201 tttagggcct ttatggaaag gtctatgcgc cctcgctcgt cgatctttat cacccgggcg
    19261 gtgatgacct gccccagctc cagcacgtcc tcgaccttct tgatgaagct gtgggagagc
    19321 tcggagatgt gaatcatcgc cttctgtccc gtcgaggtaa gcctcacaaa agccccatag
    19381 ggggcgatgt gctccactgt tcccgtgaca tcgtctccca ccttcaccgg aacaacagaa
    19441 gtttctcgtt cttctaccat gcgcgcttca gcctccaacc caagatgggc gccccccagg
    19501 gcgccggtga tgccccttca tcgcgtcttc gcctcaggtt aactgcgcgc cgagaaacca
    19561 cccccaccac gatctttttt gatccactgc caggcaagtc cctggccgct cccctgaaaa
    19621 cgccgcacta gcaccaaaag cccccccatc ggcacgcccc cctcggggcg cagccatagg
    19681 agcaattccc cgctaccctt gtctaggacg ctacgctgta ccgacttttg taccaccgcc
    19741 tcgaagatgg ccaacacggc cccgtccacc ggcagcttcc ttcctcgaag gaagcgcgct
    19801 ccgttgcgcc gacgctgggg gatgcgatcc agaagggttt tgctcccccg gcgcaccgtc
    19861 caaggattcc aagtttccca cgccgcccgg cgcaccccct ccgagggaac caaaaggggc
    19921 acccccacca ccgaagggga gaagggtccc accttctcgc cgtgcacggg caccgaacct
    19981 cccaaaggaa cgccaaaaac ctcgccgccg cgcccggagc cttttccgta ggcccgggcc
    20041 cccaggaccc ggacctcccg atccagggta tgccacacga gggccctgcg gggcatggca
    20101 tgaatgaatt ccctcctctc cggagggaaa aaggatcccc gtacctgggc ccgaccgaag
    20161 aaaaggcgtt ttaacacctc ccgcacccga tccagcgtct ctcccactac ccccgggcct
    20221 cctcgtcgat ggcctgaagg gccttgacaa tgcgcccaaa gggctctttc acgtccatgg
    20281 tctccgccgc cctccccaga agggccacca gatcccccac ccgggggagg tcctcccggg
    20341 tcacatccag acgaagagga gccgccagct cggaaagctg cacgagaaag gtcgtttcgt
    20401 cgccaaaggg gcgcacctcg ccggcgaaat ccaaaggatc atgggaactt tcctgcagga
    20461 gcaccgccgg gacgcccccg agagatcgca gaggtctcag cagctccctg gcggagcaga
    20521 tccaaagtcc cggaggaaac tccaccgccg ccaggaggtt ggtgaggatg atctcctccc
    20581 ggaggtagac ctccagcgcc cccccgtaaa gcacacgctc tagccgggaa ggagagatgg
    20641 ggtcggagta gcgaaagtct ttggggatcc cccgaaaatc cgtcaccagg gcggcccccc
    20701 ggaaaacatc ccgggcgtcg tccacccaga gatagcccaa agaatcccct cgcccccccc
    20761 cggaagaccg atccgtcttt tttgatccca ggccgctcat gcgtaaaagt ccaatccctt
    20821 ccgggagggg gacgccttgg agccctcctc aggatcctcc tcgatttctt ccggtgaagg
    20881 gcccgtcggg gatctctttc ggcgacgccg ctcccccgtg gattcttctc ggcggcccgt
    20941 cacccggttt tccgcccccg cctcttgccc ctcctggact cgctggtccc gcgcctggga
    21001 ctcctgcgcc atttctccct gctgggccgc ctgggccgcc gccgccgccg gatcccgggg
    21061 ctgttgggtg gcgttttgct ccgcccgcca cacggaaagc tgcatttcca cggggcgaat
    21121 catgcccctc ccccctttta tctatacccc ccgtctactg gaaggggcgc ggctttactt
    21181 ctccgtcgat gtacatgaaa gagatgaagt ccatcttgtc tcgcaccaga taggtgaccc
    21241 cccgaatcgt caccgccacc cccgggtaga cgctccggtg aaccgacacc cgcccctcgg
    21301 tgcgattccg atccatgtca acctcgagcg cggcgagctt gtccttgatg acgcccagct
    21361 gggcctggag ctggaactgg gcccgggtca acttgaggag gacttcccgt ttctggtcgt
    21421 ccagcctccc cagggcctcg atttttttca gatatccgac gttggagacg atctccccca
    21481 gcttgccctc cagctccttc tgattctgtt ggagttggct cttttcctcc aagatctccg
    21541 ggggaattcc caccaccact tcggtcctgg tgcccatttc gtttcccagg acttcgcacc
    21601 ggatctcgca ccccgcttgg atctttcccc ctacgatgat gccctttttc cccgtgatgg
    21661 tgttcaccgt gccccgggct cccacgtcgc tgtgcatgat gacgtcggcc accaccagat
    21721 ctcggccgca acgaacccag gcctggtcga cataggtcgc cgagaggttc cctttcacct
    21781 gcacccgggc cttccccatg ccccggagcc ctccccgcac gaccatgtcc ccttcgcagg
    21841 ccaccgtggc gccttccacc acgcccccca cggtgatgcc ccctccggcg gacacctcaa
    21901 agccctcgcg gatggtccct ttgaccacca catcccccac gaactggatg tttcccgtac
    21961 cgtagtccac gtccccggac acctcgaaga ccggcagcac gcagacccgg ttgtccttta
    22021 tcttcacgca accgtcgatg acggaaaaaa ggtgaagccc atcgtcggag atgacggtgt
    22081 tcgtgcctgc ggggatgttt ttgtccttgg ccaggcgggc cttgagcgtc ttaccaaaga
    22141 cgctcgtgcc gtcctgggag atcacggcgg gctttttttc taaaagctcc tgccccttga
    22201 tcacgttcac caccgtaccc aggtctttca tgtccacccg ggagtcgtcg acggccttgg
    22261 ggcgcgcggg ctccaggtcc accttgaaga tgaactccgc atccttgccg tccacaggag
    22321 ccactccccg ggccaccccg acccactccc cggcccgccg atcttcgatg gtcttccgaa
    22381 gggccccttc gtctatcccg aaagaaaccc ccttacgccc catctcggcc ttacaggcct
    22441 ccaccgtggg ccaaggcttt cccaggggct ctgtgatgcg gagctccgcc ttcaacccgt
    22501 cgtcactcgg tctcacttct atcatggcgg gacggtcgag gtcgtcgcga cgatcggcga
    22561 tgcgcacggg gtcctggggc ttttcgttca ccgcccgcag caccgcttcg gcgtcatagt
    22621 cctgaatgtc tcgggagcgg atgtatgcga ggacctccgc gagtaccact tccttagcgg
    22681 tcccttccac cgcgaggaag accccttccg gtttgaccac cagacggaac ggccgattac
    22741 ttcgcatacc tacgcctcct tttcgcgcgc gacacgaaag atttccctct tccgcaaagc
    22801 cctccgaaac cccgaggtca aagaaaggct agatcgttcc ctctttacgg agctcgaaac
    22861 taaggccacg gcgcggaccc ctcctagcgg agcaaggccc gcagggtggc gatggatttt
    22921 ccgtggatct gagacacccg ggactccgtg atccccagaa ccaagccaat ttctttcagc
    22981 gtcagcccgt ccccgtaata gagtgaaagc actaactgtt cccgctcttc cagagatcgc
    23041 accatggcca ttacccggtc cacgtcctcc cggtgctcca ccacttcgct gggcaagggg
    23101 gcatcgtccg ccaacatggc gctacggtcc agctccccat cgtcgaaggg caccgtttcg
    23161 tccagggacg ccatgtagct cctccccgcc acttccagga tctcccggaa ggccccttcg
    23221 tccactccca tctccgccat gagggcccgg tcgtccacgc gacgcccggc ggtggcaaac
    23281 ttctccaggg ctctctccaa gcgctggagc ttttccctcc ccgtacgaga gatccaatcg
    23341 tacttacgca gctcgtccaa aatcgcccct cggatgcggg gtacggcaaa ggtctgaaag
    23401 gaaaaacccc tctcgggttc gaagcgctcc atggcgtcca gtagcccaaa gaccccgaag
    23461 ctcaagatgt cctcgaagtc cagccctggg gggggcgtca ctcccattcg ggaagccaca
    23521 tagcgcacca gagggaggta gcgggtcacc agggtatccc gcagagaagc ccctcgctta
    23581 tctcgatact cctgccacat aagcctctcg gaatcctgga tcacgtcaca cctcctcatt
    23641 tcaggagaaa atataccatg gcaaaggcca ccacggccat ggccgccacc aacacccccc
    23701 cctccaggag gagacgcagg tcgtttcgcc cgatggaaaa gacgggcgct cgcctaggca
    23761 cgtggaggcg cacccggtaa ggaggaggga tcttcatcac accgatcacg tcatcggcga
    23821 tgtcgaggcg cacgagataa tcccccgaag gcaacagagc aatccgggat atgggaaagg
    23881 cgggcacccg ccccgtgcgg gcgcacaggc gccccacgta cctaccgaat cccgccaccc
    23941 tgtcaaaatc gacttccacc atgtcgccca gcttcagcag gctagccggt ctcccctgta
    24001 caggatcaat gacgggggcg caatagatct gcaaagacgc gccatcttcc tccgtcagcg
    24061 ggtccttggg agggggatct ccctctttct tctctggagg gaagatctcc tcgaagcgcc
    24121 cccgctggaa gcgctccgcc cgaacttcta ccataaaatc aacatgaaag gcatcggcca
    24181 acccctcccg gaagcgcgag gcaaagccgt ccagcttttc aggcgaggaa aagaggatgt
    24241 tcagctccgt ggggcccgtc aagggggcga tgacctggtc tatttttctt aaaagaatcc
    24301 ggtctggggt agaggccacg gccaaaaggg ccgatcgcac cgcctcccac cccgcatcca
    24361 cggaaaaact ttgcggtaaa tcctgccccc ccacccagat gatccggtcc cgcacggtcc
    24421 ccgaccgtcc ctcggccacc aggcaaaagg ctcccgccgc cgtgcctcgc ttccggctaa
    24481 aaaagcggcc cttgatcgcc aagtgggtgg gcgccaaaga tcccaagacc tgccgggcct
    24541 gggcatcctc caatcccaag gtggctacgg ccccctcgat ctcctcccgg gtgcagtgca
    24601 acaacgacat gagctcttcc atggaggggg ggagagctct cccgtcctct gctccctctc
    24661 cttgagaaga gggcccttcc ggggagaaag atcaaggggg tggggcgcca cgtccccttc
    24721 ccgaggggag gtctcttcag cgtaggtcat gctctcctcc gggcccccga ctagagctcc
    24781 ttgactccca cgcccagcac ctttacggaa agattccaca agggaacgtt aaattcgatg
    24841 ctccgcccct tgtttccccc cgtgtcctcg gccaccaggg ggacgttgaa atgggccaac
    24901 cacctcttgg tggcctcgat gttccgggcc cccaccgcga gaaggccgct gtctttcccg
    24961 gggatattaa acatttgagc ccctccggcg atcttggcct ggaggtttga cggtagcgcc
    25021 cccagggccg aaagttcctg caggaggcgg ggaacagccg tatcggcaaa tttgccaggc
    25081 ttgagcacgg cccccgcatc gcggctatcg gggagcatga tgtgggccat gcccgccacc
    25141 ttgttccggg gatcgtaaat caccaccccg atgcaggatc ccaaccccag ggtgacgagg
    25201 agtgaaggca ccttgactgc cacgaggtcg gccatgccga cgtggatcgt ctgggccacg
    25261 gtcacacccc caatttctgc aggagcgttt ccagagagcc gggatcgggt accatgatga
    25321 cgttgccatt cacttcacat tccacatcct tcacctggag agaggtcttt accagaaggg
    25381 ccgtgtcccc gatcaccccg aagatggacg ccacgatatc catgacggag cccagcatgt
    25441 cgtgggccac cgccggcacc gaggcaggca taacgtaccc cgtcaacatg gatatggcgt
    25501 tgagaaaagc ccccaggata atgtttccca cctctcccag ggcgctgtcg cgcatctgga
    25561 tctgcagctc cggatccaag gtgcttatgt ccatggctat gaggagattc gccaactcct
    25621 ggcccgcccc ttcgtcgatg acaaagatca gattgcaggg gaaatccccc tcggccctga
    25681 tcagcacggc gcacaccgga gtctcgggag agccgtacct tcccgccaac tcgtagatgg
    25741 ggataagatc cgcctcgggc acccccatat ccacggctcg ccccaaaagc tgcgacaggg
    25801 ccgtagcggc gtttcccgtc ccgatgttac ccacctcgcg gacggcatcc agctgaagat
    25861 tggaaaaatc tccccactcc atctccgata cgctcctcac cgggagtgga gagaggccac
    25921 atccaggatg agcgccacgt ttccgtctcc cagaattgtc gcccccgcaa tgccccgcac
    25981 cttggccaag agcttgccca ggggcttgat gacgatttcc tgctgcccca ccagctcgtc
    26041 cacgatgaat cccaggcggc tccgaccgct gcgtaccacc accacgggat actcccccgc
    26101 atccccgcgc tccacggagg tgcccaacac cgttcccaga tctcgcaggg agaggatttc
    26161 gccccgcacg ttggtcacgg gggtaccgtg gacatatttt atgtcctctt ttctcaccag
    26221 gagcgtctcc tccacgttct ccagggaaat ggcataggtc tcttccccca cgcgaatgag
    26281 caaagccagt acgatggcca gagtgagggg gaggcggatc aggacccgtg taccctctcc
    26341 cacccgggat tccaccagga actggccgtt gagggattcc accttgcttt tcactgcgtc
    26401 catgcccacg ccccgtcccg agaggtccgt caccaccgat gcggtgctga accccgggag
    26461 aaaggtgagg cgaatggcct cctcgtcggt aaggccttgg gcctcttccc gagtcatgat
    26521 gcccttttcc accgccttgg cccggatctt ggccgggtcg atccccttgc catcatcccg
    26581 gacttcgatg atgactccgt tcccctcctg gtaggcggcg atatggatcg tcccttcctt
    26641 gggtttgccg atcttgactc gatctcccgg ggcctcgatg ccgtggtcca gggaattccg
    26701 gatcagatgc accatggggt cgccaatctc gtcgatgacg ttgcggtcca gctccgtctc
    26761 cttgccgtcg atggcgaggc gtacctcctt gcccataccc ttgctcaggt ctcgcacgag
    26821 ccggggaaac ttgtcgaaga cgaaagacac ggggaccatg cgaagcttgg tcaccagctc
    26881 ctggatttca ccggaaatcc tccccaactg ggagaggggc tcttcgaagg ccttcagccg
    26941 agcctccatg gccagccgtt cgatgcgggc ccggcctatg accagctccc ccaccaggtt
    27001 catgagtttg tccaaacggc cgatgtccac ccgcaccgtg cgccccgtgc gcttcatggc
    27061 gtccaccgcc tgactcttcg ctccctttcc tcccacggca gggccgggga ctggctcctc
    27121 ttctcccccg ggctcctccc cgttgtcagg ggcgatcttt gtctcatagg aggcatcggc
    27181 ggaaagatct tcggcggcgc cggaggatcc cccaggggcc ttcgcccgtt ccggggaagc
    27241 tttgccatcg agggccggga aaaaggcttc cagggtcacg tcgcggatct cgctgatgcg
    27301 ccccacggcg tcgcggatct tctccggagt ctcgtggctc gacaaaaaca ccacaaaggg
    27361 cttctggaag gggtcttttt ccagatcctc cacagcgggg atcgtcttga tgacgtcgcc
    27421 cagctccccc gcccgggaga ccaccatgta ggcccgggcg gccttcaggg cgcaggcggg
    27481 gtccaaggaa acccgcaatt ggtaggtgcc catcccctgc tcccgggcct ccgcaaacca
    27541 atcccgctcc tgatcggtga agggtatctg gacctccgcc tcgctctggg gcttttcctt
    27601 catatccttc gtccccccct tgacacggcc gtgaagtgtc tttaccagcg gcgcaatgtc
    27661 cagagcgcca tcggaggcgc cgccgcggac caggtcgagc cccgccgtca gacgatccag
    27721 gcaggagaag agcacgctga tgtcctcttc ttccagggta taggccccct tacgcaccag
    27781 gtccaacatg tcctccatgg cgtgcgtcag atgggtcatc tgctcaaagc ccatggtggc
    27841 cgacatgccc ttcaacgtgt gcgccacccg aaagatctcg ttgatggtcc cgtcgtctcc
    27901 ggagccgctt tccagcgcca agagcagctc gttcaggctc tgcagctgct ccgaggtttc
    27961 gtcgataaaa gcccccagat actggttcat gtccacgcct gtcacggtca tcgacccccc
    28021 gtctttcttc gttccagagg cacgctcact cctccaccaa atttaccaga gcccgcgcca
    28081 tttcgtacag aggaacttga cgatccacca aacccgcttc gaaaaccacc cgaggcatgc
    28141 cgtacaccac acaggtctcc ggcgcttcgg aaataatcgt ggccccttgg gccctgagga
    28201 cccgagcccc ttcggtgccg tcttttccca tgcccgtgag gatcgccccc accacggcac
    28261 cgccaaaaac ctttgccgca ctgcggaaga ggggatccgc cgccgggcgg acggagttct
    28321 ccggaggagc gtcggagagg cgacagacgc cgtatttccc cccctcttcg atcaccaggt
    28381 gccgccctcc ccgagcgacc accgccaacc ccggacggag gggaagaccg tcgtccccct
    28441 ccaccaccgc cagggccgaa aggtcgttca tccgggcagc aaaggacgcg gtgaacccct
    28501 ccggcatgtg ctgcaccacc accacgggca cggaaagcct ggcggggagg gcgggcaaga
    28561 tctcctggag cgctcggggg ccccctgtgg aggccgccat taccaggacc cgaggcttgg
    28621 tacccggtga gaggcgaagg aaacgcccta ccgttcccgg ggcctccacg ggacgcaccc
    28681 tgtcggaaag gggcttgggc cccccttggg gcgccaggtg agacaggtcc acctgccccg
    28741 cgaggaccac cttctgacag agctcctggg ctactttctc catgtcgagg gaaatcgtgc
    28801 cggagggttt cagcacgaaa tccaccgccc ccgccgccag gcaccgcatg gtcacatcgg
    28861 ctccctgggc ggtgaggcta ctcaccatca ccaccgccgt gggctgggtc cgcatgatat
    28921 cttccagcac ctggaggccg tttttccggg gcatctccac gtccagggtg atcacctggg
    28981 gcttcagggc tcgggcctgg accagggcgt cttcgccatc ccgggccttt cccaccacct
    29041 ccagccgggg atctcgcgag aggatatccg agatgacgcg gcgcatgaac gccgagtcat
    29101 ccaccaccag cacccggagc ggggatccct tgatcggagc gtttctcatc ggggtcctca
    29161 tgtccggcca ctcatctcct gctggcggat gaagcccccc agttcttttt ccaacatccg
    29221 ggagaccccg acgtactcga taccaacctc attaagatcc gcctccttct gtgggggggc
    29281 ccagacgatc ttccccatca cgggaaacgc cttacccccc atgggcagga gggtcaggca
    29341 cagcatatcc ttgtgcgtcg cccgggatcc ccgatactgg agccgcatcc ccccaaggct
    29401 caggtcgaga acggtgccct cccgccagcg tcccgacagg tggacggatt cgccttgctc
    29461 cataagccaa aattgtactg gcatcacggc ggtcacccgg aggtacctgc gccgctgcat
    29521 ccgctccacg tcctccacca ccctcaccca caggagggga acgtgggccg tcaagtcgct
    29581 ccgcagcgcc actccgcgca ccagaagcgg caagcgctcg tcctccaggt acacccccag
    29641 agccatgtcc cggtagacgg gcaggagccc tcccttgcga agggggtgtc ccaggccgag
    29701 caccaacccc tccaccgatt ccaagatgga agggaattgc cccttgtgca accccgcgtc
    29761 gatacggatt tccgccctct cgcccacccg gggcttcagg agagactcta tcttggctcc
    29821 gtcgagttcc atgctacctc cccagcccca ggcgccgggt caaccggaac aggaaggact
    29881 tcacgccccg ccccgcccca tcgtccgtta tattttcagg tcccaccaag cgccccgcca
    29941 cctccaaaag ggccagggaa gcccggcatc cggggaagga cgagaagaag gggcggcgtt
    30001 cctgcactgc gtcgcgtact tttttatccc agggaatctg cccgatatag ggcaccgaga
    30061 tcccgagaaa ttggttcgcc gccatctgaa tccgctccgc caccgcttcg gcctcttcgt
    30121 cggacatggc catgttgacc aggaggcgca cgtccagccc ccccgccgaa ctcatgacca
    30181 aagacttcag caccccgtag gcgtcgcgga tcgaggtggg ctccggggac gtcaaaagca
    30241 ccgtggtgtc cgacgccagg gcaaaggaaa ttatatttcg gtgaatcccc gcccccgtgt
    30301 ccaccatcag gatgtccacc tttccttcca gggcagcgag cttttctata agctccgcct
    30361 ggcgcccctg gtcgaggtcg gcgagctcct gcacccccgc tccgccgggg atcaccatca
    30421 cccgctcttc cacggtcacc aggatgtctt ccaggtcgcg ctgcccccgg atcacgtgtc
    30481 ccaggttgta tttcggcatc atgccaaaaa ggatgtccac gttggccagc ccgaggtcgg
    30541 cgtccatcac cgccacccgc catccctggc tacccagggc cagggccagg ttcacggaca
    30601 ggttcgtctt ccccacgcct cccttgccgc tcaccacggc aatggtccga atcccccgat
    30661 ttcttaaggg gtttcctccc ccggcatagg ccgacaccat ggagcgcagg agcgccgcct
    30721 gatcctccga aaagggatcg ggggtcagga tttgttcttg aatcggaccc ttcgccagcg
    30781 gagaactctc tcgcgtctcc atcatcccct acctccccac cccatcttcc cccgccacga
    30841 gccgggcgat cctatccggt tccgccacct caatgtcgtt tggcacgtcc tggcccgtgg
    30901 tgatgaacga aaggggcttt ttgaagtcga gcaccgcgtt gaggatgttg ccgtaggtca
    30961 gcgtctcgtc caatttggtg aaaaggagac aggacacggg caccaccccc atgcgatcca
    31021 ccacgtccaa tatgtctcga tatttcatgt tagccgacac caccagatga accgcgtcgg
    31081 ggcgaaagac cctgtacagg gcctggagct cttccatgcg cctcgcatct ctctggctcc
    31141 gccccgccgt gtccaggagc accaggtcgg gatcgccgtg tttcgccagg acctccggga
    31201 tctcctgggg ggaaaacacc acctcgaagg gcacccccag gatccgagca taggtccgaa
    31261 gctgctccac cgccgcgatg cgataggtgt ccgccgtgag catgaggact tttttccgct
    31321 cccacaggga gtagatcgcc gcgagcttgg cgatggtggt ggtcttcccc acccccgtgg
    31381 ggcctatgaa catgacgcgc gttcccatca aggcatcgcg cacctccgcc ccacagacgg
    31441 gaatccggga ggcgatatat ggggccacgc cctccgcccc aagagccccg caggcctccg
    31501 ccacctcccg ggcgtgatgc ggcgacagat ccgccgcctc caggcgccgg gccagctcct
    31561 cccagggcga aggggccgaa gcgacggcct cttgtcggtc ctcctccagg cgggacaaca
    31621 ccctcgccaa ggtctgatgg atctcgtcca cctgccgctt cagctcctcg tcggccgccg
    31681 gaggagaggg ctcccgggga gccgagagga ggggggccgg gaggggagaa gccccggcgg
    31741 tcgccgccgc catggccttc ccttgggaag agagctccag gtgcaccccc gggggagatt
    31801 cccggcggac ctccgaaggg gcctccttcg gccccatcgc gtcccgcacg gcgtgcttga
    31861 cttccagcag acgctggaac gccatgatcc gctccttggt ctcttcggga gcgacggcgg
    31921 cccgaggggc ctcgtccccc aagatccccg ccgtcaccag caggaccttc cggcgaaaaa
    31981 aacccaagaa tcccccccgc ttcaccacct gggtcgagag gattacggcg tcacgcccca
    32041 ggcgatcccg ggccatcgac acggcatccg catcgtttct agcctcgaag gtgatctgac
    32101 gcaccacccg catttattcc accatcccca cggacttcag ct
//
LOCUS       DAOOER010000019        31934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_1597_length_31934_cov_9.240315, whole genome shotgun sequence.
ACCESSION   DAOOER010000019 DAOOER010000000
VERSION     DAOOER010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 31934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31934
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1597_length_31934_cov_9.240315"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1868)
                     /locus_tag="PL162_04205"
     CDS             complement(<1..1868)
                     /locus_tag="PL162_04205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HQF91254.1"
                     /translation="MLRSLWSGLSGLRGHQVYLDNVGHNVANVNTLGYKKSVANFQDL
                     LYETRRGGSSPSSPLGGINPMQVGHGSIIGSIDACLTQGDVQDTGMATNAAIEGAGYF
                     ALRSGSRQIYTRAGNFVFDADRNVVQSGTGYFLQGYAMTEDPANPGHFITSAALSDVH
                     IPLGQKIPGQTTTTVGFRCNLDSRVGSYLPAGFRTQDVTSKATFNAGGIPTEYTVSFA
                     EGRTVDNFLSMEARDASGAVVGTLTLSFGGVNDAGQPVMTISGTTPPTGIFAAPATVA
                     YDATTGRIVVNNTTVGSEGTWEFNTKSVADFQVMSLPVTVSAGPPPVTQERKFLAEFD
                     DVAADGSRTIRLWGADAAGTAFGAAEVTVTMNPDGSFAAHGTPDTGWSLTDSSGAAIV
                     GSLGAPTFFASADGRSLVLKQNGTETVGVSIAQRLNNGHSAPELIYDSLGNAHTLRTS
                     WEKVDNNSWRWRAWLENDDGSVSDITVTDADGKPATGILSFDSAGRVKGDVSPTLKIS
                     FGAVGAQEGEVTLDFLGKSFGGERMNGVTQFGSAFTTRAQYQDGRAMGVLTGFAYQPD
                     GTVMGTYDNGSNLPLYRVPLALFKNPEGLERLGENILGVSLNSGEPVLTMAQEGGAG"
     gene            complement(1904..2323)
                     /locus_tag="PL162_04210"
     CDS             complement(1904..2323)
                     /locus_tag="PL162_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HQF91255.1"
                     /translation="MIEDRIAGVYGAPSLSGDSGPSKTSGNNELGKDAFLNLLITQLA
                     NQDPLNPLEDKDFIAQMAQFTSLEQMTNMNTTLERLSTMNRTAAIGLMGHEVIYGGSD
                     GRLTSGKVVGVRFDAGKIFVAVGEDETLLENIVAVSS"
     assembly_gap    2343..2442
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3048..6317)
                     /locus_tag="PL162_04215"
     CDS             complement(3048..6317)
                     /locus_tag="PL162_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein"
                     /protein_id="HQF91256.1"
                     /translation="MLRSLMSGVTGVKAHQTLLDVTGNNIANVNTAGFKKSKVTFQDL
                     LYQTNRQGSAPSDGELGGIDSQQVGLGVKVGAIETLHTQGNTEYTGNRTDLAIQGDGY
                     YVVTDGVEQMYTRAGNFILDAKSDMVHAGTGYRLQGYTMVEDAANPGTYVPGTQFSDV
                     NIPVGRKMEARQTTLAGFRCNLDSGAQGNLPVGVLAQGFSSTATLGGRRYDITVAEGS
                     TAETFFQINGLLGNDALGNPIPVNLAMKGVNAVTKLPELASDPVLLGGQMYKVAFSNA
                     TGILRFENLGEPSDAWELNLKPVMDYEVVTLQDGTRSYNYLAEFNDVSGSTTDGFKRL
                     RLWGTDYRGEQSVFELPRATDGRALDIPMGKDGTFDLSSIEPQNLTVGGFAGGAFISL
                     ATAAGGRGLLLTSQEDIVDSAQAPFGIRPDKSTAALNGRTYDVTVREGDPATGNFLNF
                     QFVDRANANNTGTLNFSFNGLVGGNGNVALTSTGFTLNGRTYTATYTPSAAGPVPASM
                     TITDTVSAETWTYTFDTNATDTTDQLNFGSATINAAGTNFRTEYDALGGGDYRITFLG
                     GTTPAEEYFSVNASNFRQKTGVIPTTSTETIGTTTYAVALGEDANVTATTRAQRFVTM
                     TFTPEGGGTPETLYLSMAGVDSEGRVQLNPNISAADTLVLGGVTFDRGSYDPATGVLT
                     LGDGTVSKTFDLGSSLRFGETVTSTVAAPGTPRTFITEFDEAGTTAFNLNLWSVVDGE
                     ALSVPPAPGPYTTTVANGAVVPGSLVATIGANVYRDDGAGNLVASDGTTYATYVAATG
                     TITWPAGAAGSAVTVDYLSAAATYPLSTVSVPVERGNDQSLKPLNQRTANVHNTKFDV
                     YDSLGMNHALEVSWEKVDSNVWRWRAWLPDDPQVSLEDNTGLIRFTADGKIDSSNITV
                     YNPHPEVRVGFGEIGAQNVAVKLDFSGESFGKAKIEGITQYDSPFTTKEYIQNGYAMG
                     ILNDFSVTPDGLVNGSFTNGKNIPMYRLPLAIFANPQGLDKTGDSCFREGANSGSAQV
                     QFATEGGAGKIIGSTLEMSNVDLTDEFVTLIKGQRGFQASARVVSTGDQVLEELINLK
                     R"
     gene            complement(6503..7177)
                     /locus_tag="PL162_04220"
     CDS             complement(6503..7177)
                     /locus_tag="PL162_04220"
                     /inference="COORDINATES: protein motif:HMM:NF014207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HQF91257.1"
                     /translation="MEAPGFWRLTGESPRPGSPSAPETFLEEALFPLRERSPRERREG
                     SGGEPRKQSFSFSGDPSSLAAGGASPVRGGFDVALASQTLGSRGSAAILEGAELLLRT
                     AVRAESHSARMIIDPPALGPVEVSVTLAADGLSASFRVESEAVRQLLLQHADALRESL
                     ARAGLPVGGFSLSVDVGTGDRRPPEASPEGRRRARNGRNDEEGEERGEPLGRLDAEAG
                     VLHWVG"
     assembly_gap    7600..7699
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7700..8751
                     /locus_tag="PL162_04225"
     CDS             <7700..8751
                     /locus_tag="PL162_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91258.1"
                     /translation="LRGSFSLGSPRGKEPRAGRPVPRGGVELHAPEGRFVSLTAGELL
                     IGRGKGRGGHADGSRLSPIGEGGHRRRRRCGDRHFYPRNPGLRGSFSLGSPRGKEPRA
                     GRPVPRGGVELHAPEGRFVPLTAGELLIGRGKGRGGHADGSRLSPIGEGGHRRRRRCG
                     DRHFYPRNPGLRGSFSLGSPRGKEPRAGRPVSRGGVELRGQGIPSGFHQIRRKILEGK
                     GELRGPFGFFRHRRGRPHHRAGDTPRQGHPGGMRAHRDPQVPGERFGGGGRALPPGVP
                     GVVPRSGRKGRGGPGKRDLREFSFSRGVKKSGQQRGAPELRPRGFRVRGGRRRARRDP
                     HGKRCPHQGQHGVKR"
     gene            complement(8887..10230)
                     /locus_tag="PL162_04230"
     CDS             complement(8887..10230)
                     /locus_tag="PL162_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HQF91259.1"
                     /translation="MIRSLWTGAAGLVGHQTYLDVLGNNVSNINTVGFRQGSPRFEDL
                     LFSTEREATGPGGGIGGVSPLQVGHGTLVQAIDIIHSQGPVVPTGIPSNLAINGAGYF
                     VVQSGDQELYTRAGNFVVDGLGRVVQSGTGAFLLGVPAVGGVIPENPGALAPIQLPTL
                     PAPGEAPGPGYEIPPRATGEVTFRCNLDKNAAAGDSHETSIVMYDPLGGSHSLRVTWT
                     PQVDPTTGDKIPNAWHWQAFLDDAPTPVGEGDLAFRGVADPDSGAPAGTVIADGTERP
                     SLSLTFPPDPQGVIPDPASVVLDFGGGGNPLDGVTQYGSPSTTRALTQDGYSRGYYEG
                     FSVGSDGTLVGYYSNDVMLPLYRIPMATVRNPMGLQAEGENNFSLSSNSGSVGYVFPG
                     TQDMGTFVGGAVEMSNMDAAAAFTELIVAQRSYQANARIVTTSDRFLQVGIELRK"
     gene            complement(10541..11242)
                     /locus_tag="PL162_04235"
     CDS             complement(10541..11242)
                     /locus_tag="PL162_04235"
                     /inference="COORDINATES: protein motif:HMM:NF015413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtE intracellular region"
                     /protein_id="HQF91260.1"
                     /translation="MAKDKNLPPEYPGEERGAPRPSELPPEERAKRRPPRKRGKAGKL
                     FLLMLLGLGVASGLHYAGAWDARPLVYALVPRVPYFGPQLTEALGIPRKYALTAPERR
                     RIELEERERMINAWASSVDEAQRSVWQLSQDLAARAALLLSREERVMQDVAARSAERA
                     GQGASDKEIKDLLKVYGEMSPRNAAQIMEGLNDTLSVALLSRMPQDQAAAILAKMTPP
                     RAAALTEKLSRSPAP"
     gene            complement(11246..11893)
                     /locus_tag="PL162_04240"
     CDS             complement(11246..11893)
                     /locus_tag="PL162_04240"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HQF91261.1"
                     /translation="MRTQPPGLFQVLERMEEIRGRFAPLDPLPEIRRPEVRKPFDEAL
                     EKGRESRAPEPSPVAPDRYDRSSPPQKPPRKGRPFRATEGPAQDWEALIPEVAERYAL
                     SPALVKAVIRAESAGNPRAESPKGAQGLMQLMPGTAALMGVEDPFDPAQNMDGGTAYL
                     ASMLKRYGGDLKLALAAYNAGPGRVDAYGGVPPFPETQQYVQRVLSLYRDHEDEE"
     gene            complement(11905..12321)
                     /locus_tag="PL162_04245"
     CDS             complement(11905..12321)
                     /locus_tag="PL162_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91262.1"
                     /translation="MGRILHTREQERDLAQAELRRCRDEEETLARRVQFLDQERQRIL
                     EDFYQLSDQCLSVRDLWYGRQRITCAEGELKAAQEELVRAQERVAQAEHELVERHRSV
                     RMMETYVHKLRETSESQDRASEQAALDDVTSARYAL"
     gene            complement(12388..13716)
                     /gene="fliI"
                     /locus_tag="PL162_04250"
     CDS             complement(12388..13716)
                     /gene="fliI"
                     /locus_tag="PL162_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HQF91263.1"
                     /translation="MTKISSRDLLDSVASRLERGDFLRVNGRVAQVVGLVLESEGPDV
                     QVGDLCEVRFRRRDTALRAEVVGFRGDRVLLMPLGDIQDIGPGCDVVALGRTLGVHVG
                     PALLGRVLDGLGRPMDGLGPVVTHEYYPLHAPPPHPLRRQGIDHPLSVGVRVMDGLLT
                     LGRGQRLGIFAGSGVGKSTLLGMMARYTEADVNVIGLVGERGREVKEFMDRDLGPEGV
                     RRSVVVVATSDQPPLVRLKAALTATAVAEYFRDQGANVLLMMDSVTRVAMAQRDVGLA
                     VGEPPATRGYTPSVFAFLPRLLERAGAGERGSITGIYNVLVEGDDMNEPVADTVRGIL
                     DGHVVLSRSIAARNHYPAVDVLRSVSRVMPAVATPEHIAAAGRVREVLSIYEEAQDLV
                     NIGAYKAGSNPKIDWALANLDAVQTFLRQDVAQHSPFDVTVAQLAKLLPS"
     gene            complement(13697..14596)
                     /locus_tag="PL162_04255"
     CDS             complement(13697..14596)
                     /locus_tag="PL162_04255"
                     /inference="COORDINATES: protein motif:HMM:NF014196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HQF91264.1"
                     /translation="MSRSGGHQRLFRAVRLLPEVVRVGASLEGTPGIPEDPLAAPPPE
                     EGPQDQEIRLLKEELQRGRDEREELQGALRASARREEEARRSLEELRRQGEQREQELR
                     NQLAEELRRQEGEARTRGHQEGHQAGYAEGQNRALREVEATYRARFSDLVQLLEGVHQ
                     ELQESLEELSELSQPRLVRLWAVVLGKLLMREVAVNEATAMILLRQVLSRVSNRERLV
                     VYLNPADAGVIQESQNDLGEVLRGVRHLEFVSDGQIDRGSCLVETNLGVYDARWRTQL
                     EQINREIDGALLEERPENDENQQ"
     gene            complement(14589..15599)
                     /gene="fliG"
                     /locus_tag="PL162_04260"
     CDS             complement(14589..15599)
                     /gene="fliG"
                     /locus_tag="PL162_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HQF91265.1"
                     /translation="MAIKKELRGRDKAAVLLVVLGPEVASQVYKHLDEATIELLTLEI
                     ANLRQISPEVKHAVLKEAQEMLMAKEFMSQGGVDYARALLEGALGSERAQEILRRITA
                     SLQVRPFDFMRHTDATQVLGFIQGEHPQTVALILSYLTPEQAAAIMGGLPSNMQWDVT
                     RRIATMDRITPEVLREVERVLERKLSSVLGQDFTVAGGVDAVVDIINRVDRGTERNIM
                     ETLEEKDPDLAEEIKRRLFVFEDIIGLDDRSLQRVLREVDVKDLSLALKGATEELRQK
                     FFKNMSKRAAEMLNEDMQFMGPVRVRDVEESQQKVVNVVRALEDAGEIVIARGGEEEL
                     VV"
     gene            complement(15653..17218)
                     /gene="fliF"
                     /locus_tag="PL162_04265"
     CDS             complement(15653..17218)
                     /gene="fliF"
                     /locus_tag="PL162_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HQF91266.1"
                     /translation="MENLRQSLNRLRLFFRGLAPWQRWSLVGAAVLVSAALLLLVLWS
                     GRTTYEPLFANLDVDDQAAIVATLREQKIPYRLDPAANAILLPRDLVYETRLSLAQGG
                     LPKGGGVGYELFDQAKMGMSDFHQRVTWLRALEGELGRTIQQLDAVEFARVSIVVPQQ
                     QLFLEQQEPSTASVLLRLASGKKLGPEQVKAVVHLVARSVEGLQPDNVTVVDTAGSVL
                     SDMIGDENLLYGTGPGGQGVSSVQRELERQQELEFERKVRQMLERVFGPGKAVVRVRV
                     ELDFDKRRSSQKEFIPGETGKGVVRSQQNMEESYKGPGGVPGAPPGTTTNIPGYVVSA
                     QGQGNSEYNKGETVTNYDITTRESEQVGTPGTVRRVSASVLVDGDLKPEQVDGLKDAV
                     AAAIGLDPQRGDRLVIQPMQFSTSFVDGMLAQLQAEQRQQLILTLLTLAMLLLALALL
                     GVWWMRRRKRLAKISSQMEGEKMPSLKELMEHPELVGGQSEVVVLEEQLRIYAMKNPE
                     DVASVVKNWLSED"
     gene            complement(17234..17545)
                     /gene="fliE"
                     /locus_tag="PL162_04270"
     CDS             complement(17234..17545)
                     /gene="fliE"
                     /locus_tag="PL162_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HQF91267.1"
                     /translation="MDNAYVVNLLQRTLDSPASPSPTTVASAPSGSSFAAIMEQSMAR
                     VNDLQQEADRMTQRLALGDVDDISDVVIATSQAELALRMMVEVRNKLVDAYQQISRMP
                     V"
     gene            complement(17876..18301)
                     /gene="flgC"
                     /locus_tag="PL162_04275"
     CDS             complement(17876..18301)
                     /gene="flgC"
                     /locus_tag="PL162_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HQF91268.1"
                     /translation="MRVFHSLDVSGTALTAHRLWMDVISENLANVNATRTAEGEGPYV
                     RRVPIFAERLDRELGGAKSGSGGVKVASLVKDPLPPRLVYQPEHPDADDNGYVAYPNV
                     NIVREMADLLVASRAYESNLAVVDSAKNMWNGALEVLRG"
     gene            complement(18301..18702)
                     /gene="flgB"
                     /locus_tag="PL162_04280"
     CDS             complement(18301..18702)
                     /gene="flgB"
                     /locus_tag="PL162_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HQF91269.1"
                     /translation="MVRDLTWDVVGKDLQGLARRFGVVAENMANGNTPRYARKEVSFE
                     DQLQEVIEAPKRLPMVVTDPQHIKNVHTSVEEVAPLSRRVYDEVYRLDGNNVDPEREN
                     ALLAQSRMGYLAMNRLLAKRVGAYKLVIGGR"
     gene            complement(18866..19708)
                     /gene="codY"
                     /locus_tag="PL162_04285"
     CDS             complement(18866..19708)
                     /gene="codY"
                     /locus_tag="PL162_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HQF91270.1"
                     /translation="MTTPRELVESRPKILNASAMNDLLEKTRLVSRALQNRRNPSHPD
                     YQRLARLLCDLSAANVYLINREGKVLGYSWVSQYDCFTMARLLDEGRMPEDYLERLNQ
                     IHESVLNHDDHGMCAYADQPCTYSNKHVLYVPIYGGAERLGTLILARFGQPFDTRDLV
                     LAEYLATVVGLEILHERTRSIEERSRERLVVQMAMRALSYSEVESVRHIVRELGASEG
                     VVVASRVADRVGVTRSVIVNALRKLESAGIIESRSLGMKGTFIRISSALFLEELGVGE
                     GGSF"
     gene            complement(19914..21341)
                     /gene="hslU"
                     /locus_tag="PL162_04290"
     CDS             complement(19914..21341)
                     /gene="hslU"
                     /locus_tag="PL162_04290"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HQF91271.1"
                     /translation="MTTNPKALSEELTPGEIVAYLDRYVVGQTKAKRTVAIALRNRMR
                     RRHLPEELAREIAPKNILMVGPTGVGKTEIARRLARLVEAPFVKVEATKFTEVGYVGR
                     DVESLVRDLVESAVQMEKRRRTEEAQEGAMAAAEERLLDALLPSPRSSRRGGVPDFAR
                     LLGSLDGAGEEEPSPEEEPRSSATRERLREMLRDGRLEGREVDVEVTESPRTGMPLLG
                     NMGMEEMGINLGEMLGNLLPKRVRRTRMKVAEARRVFQAEEAEKLVDMETVLREALSR
                     AQEEGIVFLDEIDKIAAGEGGRGGGPDVSREGVQRDLLPIVEGCVVQTKYGPVHTDHV
                     LFIAAGAFHKVKPSDLVPELQGRLPLRVELDSLGEEDLFRILQEPESSLVGQYQALLG
                     VEGLELRFDEEALREVASFAARMNAEMENIGARRLQTILELLLEEISFDAHARRGETV
                     VVDASLVRRRLEGVISDGDLRKYLL"
     gene            complement(21338..21868)
                     /gene="hslV"
                     /locus_tag="PL162_04295"
     CDS             complement(21338..21868)
                     /gene="hslV"
                     /locus_tag="PL162_04295"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HQF91272.1"
                     /translation="MFKGTTVVCVRRQGRTALGADGQVTLGSQIVKGNARKVRRLHKG
                     TVLAGFAGGTADAMTLLESFEKRLEEQSGHLLRAAAFLVKEWRTDKVLRRLEAMLLVA
                     DREQTLLLSGSGDILEPEGPVAAIGSGAGYALAAARALCEASEWEADRVARRSLELAS
                     EICIYTNAAVTVEVLA"
     gene            complement(21868..22770)
                     /locus_tag="PL162_04300"
     CDS             complement(21868..22770)
                     /locus_tag="PL162_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HQF91273.1"
                     /translation="MMGHIPQAVDRFLSHMDALLGRSEHTVTNYAVDLGQFADYLQQE
                     GIEDPKEIQGDHVRGFLRALLGFGYAKTSAARKLSAVKSWLEFLRAAGELDRNPAVGV
                     RPPRPPARLPRALAYEDVKALLEGIGEDPGDRRDRAIVELLYGAGLRVAELVALDWED
                     VDLEERWLRVRGKGDKERLVPMGYPAREALRHWRVSEEGPVFPGEGAKRLTVRTVHRV
                     VVRAAHRAGLAGVTPHVLRHSFATHLLEGGASLRVVQDLLGHESLVTTQRYLRITIDQ
                     LRRSYSAAHPRAGNDGGDREDGDL"
     gene            complement(23017..24366)
                     /gene="trmFO"
                     /locus_tag="PL162_04305"
     CDS             complement(23017..24366)
                     /gene="trmFO"
                     /locus_tag="PL162_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HQF91274.1"
                     /translation="MTATHSPSIVVVGGGLAGSEAAWQLARRGYGVKLVEKRPGVPSP
                     AHRSEALGELVCSNSLGANELSSPGGILKAELRRLGSLILACADATAVPAGRALAVDR
                     ELFSRRITAVLSTHPRVEICREECEALPPEPCIVATGPLTEGRLAEDLKRRTGGFLSF
                     YDAISPVVTAESLDRDVVFRASRWGKGDDYLNCPLDAAVYQRFVEALRGAERAPLHSV
                     DQKAEYFEGCLPVEVIASRGGDTLRFGPMRPVGLPDPRTGREPHAVVQLRQENREGTL
                     YNLVGFQTNLRWGEQERVFRLIPGLERAEFVRWGVMHRNSFVRAPEVLDPLLRLRGSG
                     NCFLAGQLCGVEGYVESTAMGLVAAIHLATLLSGTLPSPWPRETTLGGLLAYLQESPP
                     DHFQPINCHLGLFPSLERRIKSRGERCLAVAARSRDALERFLSENPLAEEGEGLLGA"
     gene            complement(24363..>24573)
                     /locus_tag="PL162_04310"
     CDS             complement(24363..>24573)
                     /locus_tag="PL162_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91275.1"
                     /translation="DSDAEAPKAPSRRRVAGALLEDGKVPATPKKAPTRKKAPLEVPE
                     DLDSDAEAPKAPSRRRVAGASGEGA"
     assembly_gap    24574..24673
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<24674..26916)
                     /gene="topA"
                     /locus_tag="PL162_04315"
     CDS             complement(<24674..26916)
                     /gene="topA"
                     /locus_tag="PL162_04315"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HQF91276.1"
                     /translation="MAEKTLVIVESPAKARTLGKILGRGFSIAASMGHVMDLPRSKLG
                     IDIENGFEPQYIPVKGKAKILKELKAQAAKSDRVLLASDPDREGEAIAWHLAQALGVD
                     GRTSCRIRMYEITPKGVKEAVAAPEPIDASRVEAQQARRVLDRIMGYSLSPLLWKKIQ
                     PRLSAGRVQSVALRLLAEREREIEGFVPREYWLVDVEAEGDGGRSFTLRLEKRSGKGW
                     APESEEEARSAEAVLRREALTVSSFREKESRRPPLPPFKTSTLQQEAARRLGFAPRKT
                     MTVAQQLYEGVELPGRGPVGLITYMRTDSLRLSGDAVAMARRFIEKTHGESYVPPVAM
                     AYVAKGRSQDAHEAIRVTDVTLTPEELKKSLSGEQYKLYDLIWRRFVACQMAAAIVAS
                     TTVEATCDIYGLKHTGARLLFDGWGKVWPLDLKEESVPQAREGETLEVRSVSTAQRFT
                     TPPPRFAESGVVKALEEKGIGRPSTYAAIIQTLYDRGYVERNEEKRLQPTGLGLAVSD
                     FLVAHFPGIVNTEFTAGMEDELDDVEGGRRPWRGVVEGFWGPFSSQLREAEKNAQRVM
                     LPVREIGEQCPECGSPLVIKRGRFGEFIACSGYPQCRFSRPLLKSIGVSCPTCQTGDV
                     VRRRSRKGGRVFYGCSRYPSCDFVSWLQPAGGSCATCGGFLAVRGRSKTPFCPACAGI
                     KVRGEAASDSGEEGLRTPPLAPEKGRGAKKSEAAGESKKKAVRRVAGSLLEDGKVPAT
                     PKKAPTR"
     gene            complement(26924..27202)
                     /locus_tag="PL162_04320"
     CDS             complement(26924..27202)
                     /locus_tag="PL162_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91277.1"
                     /translation="MSIRILHPRECSALTPETWRLVAVYLGIQPHSDSALERSLEGAM
                     VLSQAEGVPVGVDAFRTLFSCGEAPMPGRAPWDEEGDGVYLSATRWAS"
     gene            complement(27238..28362)
                     /gene="dprA"
                     /locus_tag="PL162_04325"
     CDS             complement(27238..28362)
                     /gene="dprA"
                     /locus_tag="PL162_04325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HQF91278.1"
                     /translation="MDGMAEGALFLLTLGAAGATNARIWQELSSRGSSWQELVEPGSD
                     ALWTALGVPPGTVRRFRGLAEGCFAEKEAERAAFQGVHLVTAADPSYPPALWDLADPP
                     PALYVRGKWPLDSGGVAVVGTRRCTSYAFQVAQEVGRRVARAGRWLISGGAMGVDGGA
                     HGGALESGGPTAAVFGTGVDVFFPAEHGDLFRRIVAQGGTLLSEFPLGTTGRPWQFPQ
                     RNRIIAALASRTVVVEAPGRSGALVTARRAMEMGRELWVVPGRIDENVCEGSNALLAD
                     GGFPLVDVAHFAETLGGQPLQGTLGLYGDEKYDLPSDLGDLERKILAQLRDRGDQTID
                     NLATGGTMSSAHISSALALLNVRGLVYPSGPGRWRASLKK"
     gene            complement(28377..29879)
                     /locus_tag="PL162_04330"
     CDS             complement(28377..29879)
                     /locus_tag="PL162_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HQF91279.1"
                     /translation="MGVVRGVTLRGVDAVPVEVEVEITGGLFVISVVGLPDAAVREAR
                     ERVRAALRSSGIPLRGRVAVNLAPADIPKEGALLDLPMALGMAVASGALPSPPPALYL
                     GEVALDGRLRRGRGAVPAALLARQMGLPLFFPRENSSEVALVDGVEAFAVDSLPSLME
                     HLRGGAPLSPLERRTLSPGEPRVDTDLEDVKGQSGARRALEIAAAGHHNLFFVGAPGS
                     GKTLLARALRGILPPLSSEEFLEVLRVRSADGQEEIPDLQPPFRAVHHTASTAAVCGG
                     GSALRPGEVSLAHRGVLFLDELAEFRRDLLEALRQPLEDATITVSRATGSVTYPSRVL
                     LVGAANPCPCGFWGDVIQPCRCAPQERERYRRRLSGPILDRIDLHVAVPRLSPEELVG
                     LGDRRGESSARVRSRVIRAREIQRRRWEPFGYRCNAEIPERLLRRETPLGPTEAAFLR
                     DAARRMTLSGRGLSRLLRVARTIADLDGDGAPLGVSHLAEALGYRPGALW"
     gene            complement(29858..30319)
                     /locus_tag="PL162_04335"
     CDS             complement(29858..30319)
                     /locus_tag="PL162_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HQF91280.1"
                     /translation="MKDARKVKDDEGSLPTGPGDQIPSAPSPADLGRQGEEEAARYLR
                     SLGWTILGRNLRAGKSEIDLACRMGKDMVFVEVRVRSLGKWQPPETTVGPRKVAHLVR
                     GARILAERWHWEGPWRIDLVAFTVDRKGVWRREHLEDITAGGWDPWGSCGG"
     gene            complement(30316..30606)
                     /locus_tag="PL162_04340"
     CDS             complement(30316..30606)
                     /locus_tag="PL162_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HQF91281.1"
                     /translation="MAEKPRTERFKAAALQYDRSRDRAPRVVASGKGVVAERIRDLAL
                     EAHIPIVEDAALVSALLVLEIGDEVPTELYEAVAKVLSFVYRLDKERRGVRS"
     gene            complement(30610..31641)
                     /locus_tag="PL162_04345"
     CDS             complement(30610..31641)
                     /locus_tag="PL162_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91282.1"
                     /translation="MVRGIGDLPPSGIPPRGTEGFPRPIDGGRGRDGAPPLVHGALVE
                     GTVVALEGEEAYRIQVAGRFLTARSTVPLSVGQRFRAVWDASGDIPMLRLQESDQALL
                     GRISPQDRDLAEALLSRGLPLSKDVLSSLRGAWLRLGGGSERLGPMVELWARHLPLTE
                     ENLRILAWYATLDNSEVRRHWRRIRDQLKDPATPREGYLRGRLEDDELRRFATATGLL
                     ATPAREGVDPSLLTPVRWPLDDEEPLPAKVSAQVEETKHGKVYRVAFEVEGEALGVVG
                     GEVTSDGVQLAVNLRPEDPMMASLLRRRLPELERDLADGALRLVRAGVTKPRPGGTPT
                     RLRRVDLEA"
     gene            complement(31642..>31934)
                     /locus_tag="PL162_04350"
     CDS             complement(31642..>31934)
                     /locus_tag="PL162_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HQF91283.1"
                     /translation="PERPHLVVVDGTVEIPGLPLPQRALPRGDDRVLCVAAASVVAKV
                     LRDRIMERLGRLFPEYGFGAHKGYPTEAHRRALALYGPSPVHRRSFSWGEGS"
BASE COUNT         5256 a        10798 c        10040 g         5540 t
ORIGIN      
        1 cccgcgcccc cctcctgggc catggtcagg acgggctccc cggagttgag gctcaccccc
       61 aggatatttt cccccaggcg ctccagccct tcggggtttt tgaagagcgc caggggcacc
      121 cgatagagcg ggagattact gccgttgtca taagttccca tgactgtgcc gtcgggctga
      181 taggcgaaac ccgtgagcac ccccatggcc cggccgtcct ggtactgggc ccgggtggta
      241 aacgccgacc cgaactgggt gacgccgttc atgcgctcgc cgccgaagga cttgcccagg
      301 aagtcgaggg tcacttcccc ctcttgggcc cccacagcgc cgaaggagat cttgagcgtg
      361 gggctcacgt cgcccttcac cctgccagcg ctgtcaaagg acaagatccc cgtggcgggc
      421 ttcccatctg cgtccgtcac cgtgatgtcg ctcaccgagc cgtcgtcatt ttccagccag
      481 gcccgccagc gccagctgtt gttgtccacc ttctcccagc tcgtccgcag ggtatgggca
      541 ttccccagag agtcgtagat caactccggg gcgctatggc cgttattcag ccgctgggcg
      601 atggacaccc ccaccgtttc cgttccgttt tgtttcaaca ccagggaccg cccatcggcg
      661 gaggcgaaaa acgtcggagc tccaagactc cccacaatcg ccgctccgct gctgtccgtc
      721 agggaccagc cggtgtcggg ggtaccgtgg gctgcaaagg aaccgtcggg gttcatcgtc
      781 accgtcacct cggcggcccc gaaggccgtt cccgccgcgt cggcccccca caggcggatc
      841 gtccgacttc cgtccgccgc cacgtcgtca aactccgcca agaacttgcg ctcctgggtc
      901 accggaggag gtcccgcgct caccgtcacc ggtagggaca tcacctgaaa atcggcaacg
      961 ctcttggtgt tgaactccca ggttccctcg gagcccaccg tggtgttgtt cacgacgatc
     1021 cttcccgtgg tggcgtcgta ggccaccgtg gcgggagcgg cgaagatccc cgtggggggc
     1081 gtggtgccag aaatcgtcat gacgggctgc cccgcgtcgt tgactccccc aaaggagagg
     1141 gtcagggtgc ccaccaccgc cccgctggcg tcccgggctt ccatggagag aaaattatcc
     1201 accgtgcgcc cctcggcaaa ggacaccgtg tactccgtgg gaattcctcc cgcgttgaac
     1261 gtggccttag acgtcacgtc ctgggtgcga aatcccgcag ggagatagct ccccacccgg
     1321 ctatccaggt tgcaccggaa gcccaccgtg gtggtggtct gccccgggat cttctgcccc
     1381 agggggatgt ggacatcgga gagggcggcg ctggtgatga aatgcccggg gttagccggg
     1441 tcctccgtca tggcgtaccc ctggaggaag taccccgtcc ccgactggac cacgttccgg
     1501 tccgcgtcga agacgaaatt tcccgcccgg gtgtagatct gccgggagcc gctccgcagg
     1561 gcgaagtacc ccgccccctc gatggcggcg ttggtggcca tccccgtgtc ctgcacatct
     1621 ccctgggtga ggcaggcgtc gatggagccg atgatggagc catgccccac ctgcatgggg
     1681 ttgatcccgc cgaggggtga gctgggagag ctcccccctc ggcgggtctc gtagagcaga
     1741 tcctgaaaat tcgccacaga tttcttgtag cccaaggtat tgacgttggc cacgttgtgc
     1801 cccacgttgt caagatagac ctgatgtccc cggagcccgg agagccccga ccacaacgaa
     1861 cgcaacatgt tctatcgctc ccttccggta ccgggtccgg cggtcaactg ctcaccgcca
     1921 cgatgttttc cagcaaggtc tcgtcctcgc ccacggccac gaaaatcttg cccgcgtcga
     1981 agcgcacccc caccactttc cccgaggtca gccgaccgtc ggagccgccg tagatcacct
     2041 cgtgccccat gaggccgatg gcggcggtgc ggttcatggt ggaaagcctc tccagggtgg
     2101 tgttcatatt cgtcatctgc tccagcgagg tgaactgggc catctgggcg atgaaatcct
     2161 tgtcttccaa gggattgagc ggatcttggt tggcaagctg ggtgatgaga agattgagaa
     2221 aggcgtcctt tcccagctcg ttgttgccgg aggtcttcga agggcccgag tcccccgaaa
     2281 gggatggggc gccgtaaact cccgcgatgc gatcttcgat catggcgtgt cgcctccttg
     2341 ctnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnggcgccgt aaactcccgc
     2461 gatgcgatct tcgatcatgg cgtgtcgcct ccttgcttgt ttccgctggg ggtagtttcg
     2521 gctattccgg aggaaaacac aagggatcct tggcttccgt cggagaaaaa aattttctcc
     2581 cggaaaatct gcggggttcg gtctgaagag ggtgcccaaa gggggcgatg ggatcaatgc
     2641 gacgggatca aaaaggtccg gaggaaagat gcgggctctc ggccatgtgc tctccggctg
     2701 atttcttcgg tggagtacga gggggggccg gcatggccac gcaaccttga ataaagtagg
     2761 ggcgaaaaat acgatcgccc ctacagatgc ttctctgtgt tgccagtgta agccacggat
     2821 gttttcctgc gttgccggcg tgggccacga tacgaaacga atgaaagccg gctggaaggt
     2881 gcgcgctccc agctatgcga tctccggctg gtttctttga tgaagtgcgg gggggccggg
     2941 gatgaccatg gatattgatg cggaggggaa aatgaagtag gggcgaaaaa tactatcgcc
     3001 cctacggatg tttctttgct ttgccatgaa gcgatgcgat tcgagcgtta ccgcttcagg
     3061 ttgatgagct cttccagcac ctggtccccc gtggagacca cccgggcgct ggcctggaag
     3121 ccccgctgcc ccttgatgag ggtgacgaac tcgtcggtga ggtccacgtt ggacatctcc
     3181 agggtactgc cgatgatctt ccccgcgccc ccctcggtgg cgaactgcac ctgggcggag
     3241 ccggagttgg ccccctcccg gaagcatgaa tccccggtct tgtcaagccc ctgggggttg
     3301 gcgaagatcg ccaggggaag gcggtacatg gggatgttct tgccgttggt gaaagaacca
     3361 ttcaccaggc cgtccggagt cacggagaag tcattgagga tccccatggc gtagccattt
     3421 tggatgtact ccttggtggt gaagggggaa tcgtactggg ttatgccctc gatcttcgcc
     3481 ttgccgaagg actccccgga gaagtccagc ttcaccgcca cgttctgggc gccgatttcc
     3541 ccgaagccga cgcgcacctc ggggtggggg ttgtagacgg tgatgttgga gctgtcgatc
     3601 ttgccgtcag cggtgaagcg gatcagcccc gtgttgtcct ccagggaaac ctgagggtcg
     3661 tcggggagcc aggcccgcca gcgccagacg ttgctgtcca ccttttccca ggagacctcc
     3721 aaggcgtggt tcatgcccaa agagtcgtag acatcgaatt tggtgttgtg gacgttggcg
     3781 gtgcgctggt tcaggggctt cagggactga tcatttcccc gctccacggg aacggagact
     3841 gtggaaaggg gataggtcgc cgccgcactt aggtagtcca ccgtcacagc agatcccgct
     3901 gcgcctgcag gccaagtgat agtccccgta gccgccacat acgtcgcgta ggtagttcca
     3961 tcgcttgcca ccaggtttcc cgccccgtca tcacggtaga cgttcgctcc gatggtggcc
     4021 accagactcc ccggcaccac agcaccattt gccacggtgg tggtgtaagg tcctggggcg
     4081 ggaggcaccg acagtgcctc tccatccacc acactccaga gattcaggtt aaacgccgtg
     4141 gtgcccgctt cgtcgaactc ggtgatgaac gttcggggag tccccggcgc agccacagtg
     4201 gacgtcacgg tctcgccaaa tcgcaacgag cttcccaaat caaaggtctt ggaaacggtg
     4261 ccgtcgccca gggtgagcac ccctgtggcg ggatcgtagg aacccctatc aaacgtcacg
     4321 ccccccagga ccagcgtgtc ggcggcggaa atgttggggt tcagctgcac ccggccctcg
     4381 ctgtccaccc ccgccatgct caggtacagc gtctcagggg ttccaccccc ttcgggggta
     4441 aatgtcatgg taacaaaccg ttgggcccgg gtggtcgcag tgacgttggc gtcttcgccg
     4501 agggccaccg catacgtcgt ggtgccgatg gtctcggtgg acgtggtggg gatgaccccc
     4561 gtcttctggc ggaagttcga ggcattgacc gagaaatact cctccgctgg cgtcgttccc
     4621 cccaggaacg taatccgata gtccccgcct cccagggcgt cgtattccgt gcggaaattt
     4681 gttccggcgg cattgatcgt ggcgctgccg aagttgagct ggtcggtggt gtccgtggca
     4741 ttagtgtcga aggtataggt ccacgtttcc gcagacacgg tgtccgtgat ggtcatggac
     4801 gcaggcacag gccccgccgc cgaaggggtg taggtcgccg tgtaggtccg gccgttcagc
     4861 gtgaatcccg tagaagtcag agccacgttt ccattgcccc ccaccagacc gttaaagctg
     4921 aaattcaggg tgcccgtgtt gttcgcgttg gcccggtcca caaattgaaa attgaggaag
     4981 ttccccgtgg cgggatcccc ttcgcgcacg gtgacatcgt aagtgcggcc gttgagcgcg
     5041 gcggtgctct tatccggccg gatgccgaag ggggcctggg cgctgtccac gatgtcttcc
     5101 tgggaggtga ggagcaggcc ccgccccccc gccgcggtgg ccagggaaat gaaggccccc
     5161 ccggcaaacc cccccacggt gaggttctgg ggctcgatgc tggaaaggtc gaaggtgccg
     5221 tccttgccca tggggatatc gagggcccgg ccgtcggtag cccgggggag ctcgaagacc
     5281 gattgctcgc cccggtagtc cgtgccccag aggcgtagtc ttttgaagcc gtcggtggtg
     5341 ctccccgaca cgtcgttgaa ctccgccagg tagttgtagc tccgcgtgcc gtcctggagt
     5401 gtgacgactt cgtagtccat gacgggcttg aggttcagct cccaggcgtc cgagggttcc
     5461 cccaggttct cgaagcgcag gatccccgtg gcgttgctga aggccacctt gtacatctgc
     5521 ccccccaaaa ggacagggtc gctggcgagc tcagggagct tggtgacggc gttcacgccc
     5581 ttcatggcca ggttcacggg gatggggttc cccagcgcgt catttcccag aaggccgttg
     5641 atctggaaga aggtctccgc cgtggatccc tcggccacgg tgatgtcgta gcgcctcccc
     5701 cccagggtgg cggtggaaga aaagccctgg gccaggactc ccacggggag gttcccttgg
     5761 gcgccgctgt ccaggttgca gcggaacccc gccagggtgg tctgccgggc ctccatcttc
     5821 ctccccacag ggatgttcac gtcggaaaac tgggtgccgg ggacgtaggt gccggggttc
     5881 gccgcatctt ccaccatggt atacccttgg aggcggtacc ccgtgcccgc gtggaccatg
     5941 tcgcttttcg cgtcgaggat gaagttcccc gcccgggtgt acatctgctc cacgccgtcg
     6001 gtgaccacgt agtacccgtc cccttggatg gccaggtcgg tgcggttgcc ggtgtattcc
     6061 gtgtttccct gggtgtggag ggtttcgatg gctcctacct tgactccgag ccccacctgc
     6121 tgggagtcga tgcccccgag ctccccgtcg ctcggggccg acccctggcg gttggtctgg
     6181 tagagcaggt cctggaaggt gactttcgat tttttgaacc ctgcggtatt gacgttggcg
     6241 atgttatttc ccgtgacgtc gagcagggtc tggtgcgctt tgacgccggt gacgccggac
     6301 atgagggatc gcaacatggt ggtccgcctc ctctggtatt ttttctcgtg gtttgccgag
     6361 ggcgcactcc cttgcgcgtc ccacctccgc cgttccgtgg cgaagggtcg tgggtcttct
     6421 cccgtttcct cgagaactcc cctcgaaccg atcactccct ttttcggcgg cgcggagggt
     6481 aaactgaaga aaaatcggaa gatcatccca cccagtggag cacccccgcc tccgcatcca
     6541 ggcgccccag gggttctccc ctctcctcgc cttcttcgtc gttccggccg ttccgagccc
     6601 gacggcgccc ctccggggag gcttcggggg gacgccgatc ccccgtaccc acgtccacag
     6661 acagggagaa gccccccacc ggaaggcccg cccgggccag gctctcccgc agggcgtcgg
     6721 cgtgctgcaa gaggagctgg cgcaccgcct cgctctccac ccggaaggag gcggaaaggc
     6781 catcggccgc cagggtgacg gagacctcca cgggccccag ggcggggggg tcgatgatca
     6841 tgcgggcgga atggctttcg gcccgcaccg ccgtccgcag gaggagctcc gccccttcga
     6901 ggatggcggc gctccctcga cttcccaggg tctgggaggc cagggcgacg tcaaagcccc
     6961 ctcggaccgg agatgccccc cccgccgcca aggaggaggg gtctcccgag aaggaaaaag
     7021 attgtttcct cggttcgccc cccgatccct cccgccgctc ccggggggag cgttcccgca
     7081 gggggaaaag ggcctcttcc aggaaggtct ccggggcgga cggggatccc ggcctcgggc
     7141 tttctccggt cagcctccag aatccagggg cctccacagg gccttctgta ccggcaggga
     7201 ggacgctcgg gagcgggggc gcctcgtcaa tagagggcgg accagaatca tagagggttc
     7261 cgaagagcgg tccggtcccg ggagcgaagg gccctccccg aagggggccg gcggcggggg
     7321 tggaaggccc tccggaagga gtctcggaaa tcccccgccc ccccctttcc cccggatcct
     7381 cgggggcttc tcgccccagg ggggcgcttt gtccgggagc ttctaaggtt ttgtcgaaaa
     7441 cgaaacggtc ctttgttatc cccaggtcaa aggggcgaag tccctgcggc gatggggctt
     7501 cggtcaaggt cccccttaaa gagggtggga tcaaggggcc atccccctca ccgccggaga
     7561 gcttctgatc ggccggggaa aggggcgcgg agggcacgcn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnng gctccggggg agtttctcct tggggagccc tcggggcaaa
     7741 gagccccggg cggggcgccc ggtcccccgg gggggagtgg agctccacgc cccggagggg
     7801 cgcttcgtct ccctcaccgc cggagagctt ctgatcggcc ggggaaaggg gcgcggaggg
     7861 cacgccgacg gatcccggct ctctccgatc ggggaggggg gccaccgaag gcgccgacgt
     7921 tgcggcgacc ggcacttcta cccccggaac cccgggctcc gggggagttt ctccttgggg
     7981 agccctcggg gcaaagagcc ccgggcgggg cgcccggtcc cccggggggg agtggagctc
     8041 cacgccccgg aggggcgctt cgtccccctc accgccggag agcttctgat cggccgggga
     8101 aaggggcgcg gagggcacgc cgacggatcc cggctctctc cgatcgggga ggggggccac
     8161 cgaaggcgcc gacgttgcgg cgaccggcac ttctaccccc ggaaccccgg gctccggggg
     8221 agtttctcct tggggagccc tcggggcaaa gagccccggg cggggcgccc ggtctcccgg
     8281 ggaggagtgg agctccgggg gcagggcatc ccctcgggat tccaccaaat caggcggaaa
     8341 atcctcgagg gaaagggaga gctccggggc cccttcggct tcttccggca ccgccggggc
     8401 cgacctcatc atcgcgccgg ggacaccccc cgccagggcc acccgggagg tatgcgagct
     8461 caccgagacc cccaggtccc gggggaacga ttcggagggg ggggtagggc cttgcccccc
     8521 ggggtccccg gcgtggtccc gcgctccgga agaaaggggc gcggtggtcc cgggaagcga
     8581 gacctccggg agttctcctt ctcccggggc gtaaaaaagt ctggccagca gaggggggcg
     8641 ccggagctcc gtccccgggg cttccgggtc cggggagggc gccggagggc ccgccgggat
     8701 ccccatggga aaagatgccc ccaccagggc cagcacggcg tcaaacgata gggagtcccc
     8761 ctcgcgccgc gccttgtcgt cgcccccttc ccgggggccc tcatcggcgg cgaagggccg
     8821 ggaatcccgg cccccaaaga gcattctcat gtccagaggg tccatggtct tttcccccct
     8881 cgtcccttac ttgcggagct cgatgcccac ctggaggaag cggtcgctgg tggtcacgat
     8941 gcgggcgttg gcctgatagc tgcgctgggc cacgatgagt tcggtgaagg cggcggcggc
     9001 atccatgttg ctcatctcca ccgctccccc cacgaaggtc cccatgtcct gggtccccgg
     9061 aaagacgtag cccacgctcc ccgagttgga ggaaagggaa aagttgtttt ccccctccgc
     9121 ctggagcccc atggggtttc gcacggtggc catggggatg cggtacaggg ggagcatgac
     9181 gtcgttggag tagtacccca ccagcgtgcc gtcggagccc acggaaaagc cctcgtaata
     9241 cccccgggaa tagccgtctt gggtcagggc ccgggtggtg gaggggctgc cgtactgggt
     9301 gaccccgtcg agggggtttc cccctcctcc gaagtcgagc accacggagg cgggatcggg
     9361 aatgacgccc tgggggtccg gagggaaggt cagggaaagg gagggacgct ccgtgccgtc
     9421 ggcgatcacg gtgcccgccg gggctccgga gtcgggatcc gccacccctc gaaaggccag
     9481 gtctccctcc cccacagggg tgggggcatc gtccaggaag gcctgccaat gccaggcgtt
     9541 ggggatcttg tcccccgtgg tgggatccac ctggggggtc caggttaccc tcagggagtg
     9601 cgatcctccc aaggggtcgt acatgacgat ggaggtctcg tgggagtccc cggcggcggc
     9661 gtttttgtcg aggttgcacc gaaaggtcac ctctcccgtg gcccgggggg ggatctcgta
     9721 gccgggaccc ggggcctccc ccggggcggg gagggtgggg agctggatgg gggccagggc
     9781 gccggggttc tcggggatca ctcccccgac ggcgggcact cccaaaagaa aagccccggt
     9841 gccggactgc accacccgcc ccaggccgtc cacgacgaag ttcccggccc gggtgtagag
     9901 ctcttggtct ccggactgga ccacgaagta ccccgccccg ttgatggcga gattcgaggg
     9961 gatgcccgtg ggaaccacgg ggccctggga gtggatgatg tcgatggcct gtaccagggt
    10021 gccatgcccc acctgcaggg ggctcacccc tccgatgccc cctccggggc cggtggcctc
    10081 gcgctccgtg gaaaaaagca ggtcttcaaa tcggggagat ccctgacgaa agcccacggt
    10141 gttgatgttg ctcacgttgt tgcccagcac atccagatag gtctggtgtc ccaccaaacc
    10201 cgcagctccg gtccagagcg aacgtatcac ggggacgacc tccttcctaa agtacaccgc
    10261 catgcctcgc gtcctgcgag ggcggcgcgc cgcgcctttt tgattcggcc gtccctggcc
    10321 gggccctggg tccagggctg tcgggatctc caccgatccc gtagggagta tcggccgtcc
    10381 ccggcgagac gtaaaggcca ggggaaaaga tccccgggcc ccggcggcag ggggcttcga
    10441 gctttgggga gaggcgacgt cgcaaggggc ggttccgagg gagggcgggg cgcttcggag
    10501 ggcgatgagg gaaagggccc gctcggcccc cgagggggac ctagggggcg ggggagcgag
    10561 atagtttttc cgtgagggcc gccgcccggg gaggggtcat cttggccagg atggcggcgg
    10621 cttggtcctg gggcatgcga gacagaaggg ccacggaaag ggtgtcgttg agcccttcca
    10681 tgatctgggc ggcgttgcga ggggacattt cgccgtagac cttcagcaga tcttttatct
    10741 ctttgtcgga ggccccctgg cccgctcgct cggcgctacg ggccgccacg tcctgcatga
    10801 cgcgctcttc ccgggagagc aagagggccg cccgggccgc cagatcctgg gagagctgcc
    10861 acacgctccg ctgggcctcg tcgacgctgg aggcccaagc gttgatcatg cgctcccgtt
    10921 cttccaactc gattcggcgt ctctccggag ccgtaagggc gtacttccgg ggaatgccca
    10981 gggcctcggt cagttgaggg ccaaagtagg gcacccgagg caccagcgca tagaccaggg
    11041 gacgggcgtc ccaggccccg gcgtagtgga gtcccgaggc gaccccgagg cccaagagca
    11101 tgaggagaaa gagctttccc gccttccccc gtttacgggg gggccttctt tttgcccgct
    11161 cttccggcgg tagctccgag gggcgcggag cccctcgctc ctctccgggg tactcgggag
    11221 gaaggttttt atccttggcc atggtctact cctcgtcttc gtggtcccgg tagagggaca
    11281 ggactcgctg gacatattgc tgggtctcgg ggaagggggg tacgcccccg taggcgtcca
    11341 ctcggccggg gcccgcgttg tacgccgcca gggccagttt caggtctccc ccgtagcgct
    11401 tgagcatgct cgccaggtag gccgtgcccc cgtccatgtt ctgggcgggg tcaaaggggt
    11461 cttccactcc catgagggcc gccgtgcccg gcatgagctg catgagcccc tgggctccct
    11521 tgggggactc ggcccgggga tttccggcgg attccgcccg gatcaccgcc ttcaccaggg
    11581 ccggagagag ggcgtagcgt tccgccactt cggggatgag ggcctcccaa tcctgcgcgg
    11641 ggccctcggt ggccctaaag gggcgcccct ttcggggggg cttctgggga ggggatgatc
    11701 ggtcgtagcg gtccggcgca acgggggagg gctccggagc ccgggactcc cgaccttttt
    11761 ccagcgcctc gtcgaagggc tttctcacct ccgggcgacg gatctcgggg aggggatcaa
    11821 ggggggcaaa gcgtccccgg atttcttcca tgcgctccaa gacctgaaag aggcccggcg
    11881 gctgggttct catggactca cctcctaaag ggcgtatcgg gccgaggtca catcgtccag
    11941 ggcggcctgc tcggaggccc ggtcctggct ctccgaggtc tcccgaagct tatgcacgta
    12001 ggtctccatc atgcgcacgg agcggtggcg ctccaccagc tcgtgctcgg cctgggccac
    12061 ccgctcctgg gcccggacca gctcctcctg ggccgccttc agctctcctt cggcacaggt
    12121 gatgcgctgt ctgccgtacc agaggtcccg aacggagagg cactggtccg aaagctggta
    12181 aaaatcctcc aggatcctct ggcgttcctg gtccaaaaac tgcacccgcc gtgccaaggt
    12241 ctcttcttcg tccctacagc gccggagctc ggcctgagcc aggtcgcgct cctgttctct
    12301 ggtatgaagg atacgcccca tacgcttgat cttctgccgc aatggagccc cctcccttct
    12361 ctggcttgaa agacgccgat gacggatcta cgagggcagc aatttggcca actgggccac
    12421 ggtgacgtcg aagggcgaat gctgggccac atcctggcgc aaaaaggtct gtaccgcatc
    12481 gaggttggcc agggcccagt cgatcttcgg gttgctgccc gccttgtagg ccccgatgtt
    12541 caccaggtcc tgggcctcct cgtagatgga gagcacttcc cgcacccgcc cggcggcggc
    12601 gatgtgttcc ggagtcgcca cggcgggcat cacccggctc acgctacgga gcacgtccac
    12661 ggcggggtag tggtttcgcg cggcgatgga tcgggaaagg accacgtggc cgtccaagat
    12721 gccccgcacg gtgtccgcca cgggctcgtt catgtcatcc ccctccacca gcacgttgta
    12781 gatgccggtg atgctccccc gctctccggc cccggcccgc tccaacagcc gggggaggaa
    12841 ggcaaagacc gacggggtgt acccccgggt ggcggggggc tcccccaccg caaggcccac
    12901 gtcgcgctgg gccatggcca cccgggtgac ggagtccatc atcaagagga cgttggcccc
    12961 ttggtctcgg aagtactccg ccaccgccgt ggcggtgagg gccgccttga ggcgcaccag
    13021 ggggggctga tccgaggtgg ccaccaccac cacggagcgc cgcactcctt cgggccccag
    13081 gtcccggtcc atgaactcct ttacttctcg gccccgctcc cccaccaggc cgatgacgtt
    13141 cacgtccgcc tcggtgtatc gggccatcat gcccagcagg gtgctttttc ccacccctga
    13201 accggcgaag atgcccaatc gctgcccccg ccccagggtg agcaagccat ccatcacccg
    13261 cacccccacg gaaaggggat ggtcgatgcc ctggcgccgc agggggtgag gcggcggggc
    13321 gtggagaggg tagtattcgt gggtcaccac ggggcccaat ccgtccatgg ggcgccccag
    13381 gccatccaag acgcgcccca gcagggctgg gcccacgtgg acccccaggg ttctgcccag
    13441 ggccaccacg tcgcaccccg ggccgatgtc ctggatgtcc cccaggggca tgaggagcac
    13501 ccgatctcct ctaaaaccca ccacttcagc ccggagagcc gtgtcgcgcc gacgaaagcg
    13561 cacttcgcag aggtctccca cctggacgtc ggggccttcg gactccaaca ccaaccccac
    13621 cacctgggcc acccggccat tcacccgcaa aaaatcccct cgctccagcc gggaggccac
    13681 gctgtccagc aggtccctac tgctgatttt cgtcattttc cggcctttcc tccagcaggg
    13741 cgccgtcgat ctcccggttt atctgctcca gctgggtcct ccatcgggca tcgtagaccc
    13801 ccaggttggt ttccaccaga cagctccccc ggtcgatctg accatcggag acgaactcta
    13861 gatgacgcac accccggagg acttctccca ggtcgttctg gctctcctgg atgacccccg
    13921 cgtcggcggg gttgaggtaa accaccaggc gctcccggtt gctcacccgg gagaggactt
    13981 ggcgcaaaag gatcatggcg gtggcctcgt tcaccgccac ttcccgcatg agcaacttgc
    14041 ccaggacgac cgcccaaagc cgcaccagcc ggggctggga aagttcagag agctcctcca
    14101 ggctttcctg caactcctgg tgtacccctt cgagcagctg gaccaaatcg gaaaaccggg
    14161 cgcgataggt ggcttcaacc tcccgcagag cccggttttg tccttcggcg tatcccgcct
    14221 gatgcccctc ctggtgtccc cgggtccggg cctccccctc ctggcgccgg agctcttcgg
    14281 cgagctgatt ccggagctcc tgctcccgct gctccccctg acgccggagt tcttccaggg
    14341 accttcgggc ctcttcttcc cgtcgggccg aagcccgcag ggccccctgc agctcttctc
    14401 tttcgtcacg gccccgctgg agctcctcct tcagcaggcg gatctcctgg tcctgggggc
    14461 cctcttcggg gggaggggcg gcgaggggat cctccgggat ccccggggtc ccttcgaggg
    14521 acgcccccac ccgcaccacc tcgggaagaa ggcgcaccgc ccgaaagagg cgctgatggc
    14581 cgccgcttct agacaaccag ctcctcctcc ccgccccggg cgatgacgat ttcccccgca
    14641 tcctccaggg cccgcaccac gttcacgacc ttctgctgag attcttccac gtcacgcacc
    14701 cgcacgggcc ccatgaactg catgtcttcg ttgagcatct ccgccgcccg tttggacatg
    14761 ttcttgaaga acttctgtct aagttcctcg gtggcgccct tcagggccag cgagaggtcc
    14821 ttgacgtcca cctcccggag gacgcgctgc agagagcggt cgtccaggcc aatgatgtcc
    14881 tcgaagacga agaggcgccg cttgatctct tccgccaggt cggggtcctt ttcttccaga
    14941 gtttccatga tgttgcgctc cgtaccccgg tccacccggt tgatgatgtc caccacggcg
    15001 tccactcccc ccgccacggt gaaatcttgt cccaagacgc tggaaagctt gcgctccaac
    15061 acccgttcca cctcccgcag gacctccggg gtgatgcggt ccatggtggc gatgcgccgc
    15121 gtcacgtccc actgcatgtt cgatgggagc cctcccatga tggcggcggc ctgctccggc
    15181 gtcaggtacg acaggatgag cgccacggtc tgggggtgct ccccctggat gaagcccaac
    15241 acctgggtgg cgtcggtgtg ccgcatgaag tcgaaggggc gtacctggag gctcgccgtg
    15301 atccgccgca aaatttcctg ggcccgttcg gagcccaggg ccccctccag cagggcccgg
    15361 gcgtaatcca cgcccccctg gctcatgaac tccttggcca tgagcatctc ctgggcctct
    15421 ttgaggaccg cgtgcttcac ttcgggcgat atctgcctga ggttggctat ttccaaggtg
    15481 aggagctcga tggtggcctc gtcgaggtgc ttataaacct gggacgccac ctcgggcccc
    15541 aggaccacca ggaggacggc cgccttatcg cgtccccgaa gttctttttt tatggccacc
    15601 agtctcctcc ttcccggaac ttacggcccc atatcgatgt attcgcctaa agctaatctt
    15661 cggaaagcca gttcttcacc accgaagcca cgtcctcggg gtttttcatg gcgtagatcc
    15721 gaagctgttc ctccagcacc acgacctcgc tctgcccccc cacgagctct ggatgctcca
    15781 tgagctcctt gagggagggc atcttctccc cttccatctg agaggagatc ttggccaagc
    15841 gcttgcggcg ccgcatccac cacacgccga ggagcgccag ggcgagaagg agcatcgcca
    15901 gggtcaagag ggtcaggatg agctgctggc gctgctccgc ctgaagctgg gccaacatgc
    15961 cgtccacgaa agaggtggaa aactgcatgg gctggatcac caggcgatct ccccgctggg
    16021 gatccaggcc gatggccgcc gccacggcgt cctttaaccc atccacctgc tcgggcttca
    16081 ggtctccgtc caccaacacg gaggcggaga cccgccgtac cgtcccgggg gtgcccacct
    16141 gctcgctctc ccgggtggtg atgtcgtaat tggtgacggt ctccccctta ttgtactcgc
    16201 tgttgccctg cccctgggcg gagaccacgt accccgggat gttggtggtg gtgccggggg
    16261 gggcgccggg aactcctccg ggccccttgt agctctcctc catgttttgc tggctccgga
    16321 ccacgccctt accggtctcc ccggggatga attctttctg cgatgatctg cgcttgtcga
    16381 aatccagctc cacccggacg cgcaccacag ccttccccgg gccgaagacc cgctcaagca
    16441 tctggcgcac cttgcgctcg aattccagct cctgctggcg ctccagctcc cgttggaccg
    16501 acgacacccc ctggcctccg ggccccgtac cgtacagaag attttcatcg cctatcatgt
    16561 cggagaggac ggaacccgcc gtgtccacca cagtgacgtt gtcgggctgg agcccctcca
    16621 cgctacgcgc caccaggtgg accacggcct taacctgctc cgggcccagc tttttccccg
    16681 aggccagacg gaggagaacg gaggccgtgg agggttcctg ctgctccaga aaaagctgtt
    16741 gctgggggac gacgatgctc acccgggcaa attccacagc gtcgagttgc tggatggtgc
    16801 gccccagctc cccttccaag gcgcgcagcc aggtgactcg ctgatgaaag tcgctcatgc
    16861 ccatcttggc ctggtcgaag agctcgtaac ccacccctcc ccccttgggg agtcctccct
    16921 gggccaggga taggcgggtt tcgtagacca ggtcccgggg gaggaggatg gcgttagcgg
    16981 cgggatccag gcggtagggg atcttctgtt cccgcagggt ggccacaatg gcggcctggt
    17041 catccacgtc gaggttggcg aagaggggtt cataggtggt gcgccccgac caaaggacaa
    17101 gaagcaacag cgccgcactc accagtacgg cggcccccac caaggaccag cgctgccacg
    17161 gcgccagccc ccggaagaag agcctcagcc ggttaaggga ttgtcgcaga ttctccattc
    17221 ctcgaaatta tcgttaaaca ggcatgcgcg agatctgctg gtatgcgtcc accagcttgt
    17281 tccgcacctc caccatcatg cgaagggcca gctctgcctg ggatgtggcg atgaccacgt
    17341 cggaaatgtc gtccacatcc cccagggcca ggcgctgggt catgcggtcc gcctcctgct
    17401 ggaggtcgtt gacccgggcc atgctctgct ccatgatcgc ggcaaaggag ctccccgaag
    17461 gggccgaagc gacggtcgtg ggggaaggag acgccgggga atcaagggtt ctctgaagca
    17521 ggttgaccac gtaagcgttg tccatcggga gcccctcctt gcgcgtacaa acaccgcccc
    17581 cccgatcgca gcccccgggg aagccgttca aaaatccgac gcaccgccca gtttagcatg
    17641 aagggcgaca aagtaacagg gggagcgttc actctcgccc ccccgggatc ccatcggaaa
    17701 aaaccgctga attattatgg taggggcgtc aaggggtttc gactccaaga tcggaaaaac
    17761 ccggacggcc ccccgaagga aaagatgggg cggctccttt cggcgcaaga gcccgagggg
    17821 agccgccgaa acgcccgact cccgcctctc ccgaggtgct aaaggggccc tgaatctacc
    17881 cccgaaggac ctccagcgcc ccgttccaca tgttcttcgc cgagtccacc acggcgaggt
    17941 tgctctcgta ggcccggctc gccaccagga gatcggccat ctctcgcacg atgttcacgt
    18001 tggggtaggc cacgtagcca ttatcgtccg catcggggtg ttcgggctgg tagaccagcc
    18061 gggggggcag ggggtctttg accagcgagg ccaccttgac tcccccggag cccgacttgg
    18121 ctccccccag ctcccggtcc aggcgctcgg cgaaaatcgg cacccggcgc acgtaggggc
    18181 cctccccctc cgccgtccgg gtggcgttga cgttcgccag gttctcggag atcacatcca
    18241 tccagaggcg atgggccgtg agggccgtgc cagatacgtc caaagaatga aataccctca
    18301 tcatcggcct ccgatcacca gtttataagc ccccacccgc ttggccagca ggcggttcat
    18361 ggcgagatat cccatccggg actgggccag gagggcgttc tcccgctcgg ggtccacatt
    18421 gttgccgtcg aggcgataga cttcgtcgta gacccggcgg gagagggggg ccacttcctc
    18481 caccgaggtg tggacgtttt tgatgtgctg ggggtccgtg acgaccatgg ggagcctctt
    18541 gggggcctcg atcacctctt gaagctgatc ctcgaaggag acctctttcc gggcgtatcg
    18601 aggggtattg ccgttggcca tgttctcggc caccacccca aagcgccggg cgaggccctg
    18661 cagatcctta cccaccacgt cccaggtcaa atcccttacc atgtcttcgc cccctctaac
    18721 ttccggatct cttttatccc ccgtcaccag agtatcggat ccccggggag ttccctgaag
    18781 tccctttacg accccggttc ggtctaaaac gggcaaaagg ccggggcgat gggggcgccg
    18841 ggccggggaa tctttcgggt tttgcctaaa aagatccccc ttcccccacg cccagctcct
    18901 ccaggaaaag ggcgctggag atccggataa acgtgccctt catgcccagg ctccggcttt
    18961 cgatgatccc cgcgctctcc agcttacgca gggcgttgac gatgacgctc cgagtcaccc
    19021 ccacccggtc cgccacccgg ctggccacca ccacgccctc ggaggccccc agctctcgca
    19081 cgatgtgccg cacggattcc acttcggagt acgacagggc ccgcatggcc atctgcacca
    19141 ccaggcgctc ccgggagcgc tcttcgatgg accgggtgcg ctcatggagg atttcaagcc
    19201 ccaccacggt ggccagatac tccgccaaga ccaaatcccg ggtgtcgaag ggttggccga
    19261 accgggcgag gatcagggtc cccaggcgct ccgccccgcc gtaaatgggc acatagagca
    19321 cgtgcttatt ggagtaggtg cagggctgat ccgcataggc gcacatgccg tggtcatcgt
    19381 gattcagcac ggactcgtga atctggttta ggcgctccag gtagtcctcg ggcattcgtc
    19441 cctcgtccag aagccttgcc atggtgaaac agtcgtactg gctcacccag gaatacccca
    19501 ggaccttgcc ctctcgattg atgagataga cgttggccgc cgaaagatcg cagaggagtc
    19561 gggccaaacg ctgatagtcg ggatgggagg gatttcggcg gttctgcagg gctcgactca
    19621 ccaggcgcgt cttctccagc aggtcgttca tggcggaggc gttgaggatt ttgggtcggg
    19681 actccaccaa ctcccgggga gtggtcatgg tcatggtttt ttcctcctgt cggagagtgg
    19741 tatccccctc tcctccggtg acaagccttt ctccattgcc ccctaagggg cgacgctctt
    19801 cgctcatggc caatctctcc ttaaaccgcc atctcccggg atggccctcc gcaccactga
    19861 gatcttcctc cagggatgca tcacggcaat tttcccatcg ataaataacg atcctagagc
    19921 aggtacttac ggaggtctcc gtcggagatc actccctcca gacgacggcg caccagggag
    19981 gcatccacca ccacggtctc gcctcggcgg gcgtgggcgt cgaagcttat ctcctccagc
    20041 aggagctcca aaatggtctg aagccttcgg gccccgatgt tttccatctc cgcgttcatg
    20101 cgggcggcaa aggacgccac ctcccgcagg gcctcctcgt caaagcgtag ctccaacccc
    20161 tccaccccca gaagagcctg gtattgtccc accaggctgc tttcgggctc ttggaggatg
    20221 cgaaagaggt cctcttcccc cagggaatcc agttccaccc gaaggggtaa cctcccctga
    20281 agctcgggga ccaggtcgga gggtttgact ttatgaaagg ccccggcggc gatgaagagc
    20341 acgtgatcgg tgtgcacagg gccgtatttg gtctgcacca cacacccctc cacaatgggc
    20401 aggaggtcgc gctgaacccc ctcgcggctc acgtcggggc ctccgccgcg ccccccctct
    20461 ccggcggcga ttttatcgat ttcgtccaga aagacgatgc cttcctcctg ggcccgggag
    20521 agggcctccc ggaggacggt ctccatgtcc acgagttttt ccgcctcttc agcctgaaag
    20581 acccgccggg cctccgccac cttcatccgg gtccgacgaa cccgtttggg aaggaggttg
    20641 cccagcatct ctccgaggtt gatccccatc tcttccatgc ccatgttgcc caaaaggggc
    20701 atgcccgttc gggggctctc cgtcacctcc acgtccacct cgcgtccctc gaggcgccca
    20761 tcccggagca tttccctgag gcgctcccgg gtggcggagc tccggggctc ctcttccggg
    20821 gatggttctt cttcgcccgc cccatccagg ctccccaaca ggcgggcaaa gtccggaacg
    20881 ccgccccgac gggaagagcg cggcgagggg agcagggcgt ccagaaggcg ctcttccgcc
    20941 gccgccatcg ccccctcctg ggcctcttcc gtcctccggc gcttttccat ctggaccgcc
    21001 gattccacca ggtcccgcac gagagattcc acgtcgcgcc ccacgtagcc cacctccgtg
    21061 aacttggtag cctccacctt cacgaagggg gcctccacca ggcgggcgag gcgccgggca
    21121 atttcggtct tgcccacccc cgtgggaccc accatgagga tgtttttggg ggcgatctcc
    21181 cgggccaact cctcggggag gtggcgacga cgcatccggt tcctcagggc gatggccacg
    21241 gtgcgcttgg ccttggtctg acccacgacg tagcgatcca gataggccac gatctccccc
    21301 ggggtgagct cttccgatag ggccttgggg ttggtcgtca tgccagcacc tccacagtga
    21361 cggcggcatt ggtgtagatg cagatttccg aagcgagctc cagggagcga cgggccaccc
    21421 gatcggcctc ccactccgag gcttcgcaga gcgcccgggc cgccgccagg gcgtaccccg
    21481 cccccgaccc gatggccgcc acggggccct cgggttccag gatgtccccc gaaccggaaa
    21541 ggagcaaagt ctgctcccgg tccgccacca ggagcatggc ctccagacgc cgcaacacct
    21601 tgtccgtgcg ccactccttc accaggaacg cggcggcccg gagcaggtgg ccggactgtt
    21661 cttccaggcg tttctcaaag ctctctagta aggtcatggc gtccgccgtg cccccggcaa
    21721 atcccgccag caccgttccc ttgtggaggc ggcgcacctt ccgggcgtta cccttgacga
    21781 tctggcttcc cagcgtcacc tggccatccg cccccagggc ggtgcgcccc tggcgtcgca
    21841 cgcataccac ggtggtgcct ttgaacatca aagatccccg tcctctctat cgcccccgtc
    21901 gttccccgcg cggggatggg cggcgctgta gctccttcgc agctgatcga tggtgatccg
    21961 cagatagcgc tgggtggtga cgaggctctc gtgtcccaaa agatcctgga ccaccctaag
    22021 gctcgccccc ccttccagga ggtgggtggc gaaggagtgg cggagcacat ggggggtcac
    22081 ccccgccagc cccgcccggt gagcggcccg caccaccacg cggtgcacgg tgcgcaccgt
    22141 caggcgcttc gctccctctc cgggaaaaac cggcccctct tcagagaccc tccaatgccg
    22201 taacgcctcc cgggcggggt atcccatggg taccaggcgc tccttgtccc ccttgccccg
    22261 cacccggagc cagcgctctt ccagatccac gtcctcccaa tccagggcca ccagctccgc
    22321 cacccggagc cccgcaccgt agaggagctc gacgatggct cgatctcgcc ggtccccggg
    22381 gtcctcgccg atgccctcca ggagggcctt cacgtcttca taggccaggg ctcggggcaa
    22441 acgggcggga ggacgggggg ggcgcacccc cacagcgggg tttcggtcca gctcccctgc
    22501 ggcgcggaga aattcgagcc agctctttac cgccgaaagc ttgcgcgccg cgctggtctt
    22561 ggcgtagccg aagcccagaa gggcccggag aaagccccgc acgtgatccc cctggatctc
    22621 cttggggtct tcgatcccct cctgttgcag gtagtcagca aattgcccca gatccacggc
    22681 gtagttggtc accgtgtgct ccgaccgtcc caagagggcg tccatgtgag agaggaagcg
    22741 atccaccgcc tgcggaatat gtcccatcat gctagtcagt gtatcatgtt tttgcaaaaa
    22801 gagcatactt tacacaagaa aagaaaactt tcagattttg ccattcatcg gggtaaaaat
    22861 ttttggctcc tccctcaaat cgccccgacg ccccgaggac tccggcgccc aaaaaagggc
    22921 cgtaggggcg aaaactccat cgcccctacg ccggtggtgc taaaaacttt ggcctcttgc
    22981 tgcctttttc tggcacggcg cctcaccggc cttttttcac gccccgagga gcccttcacc
    23041 ctcctccgcc agggggtttt ccgaaaggaa gcgctccagg gcgtcccggg atcgggcggc
    23101 caccgccaag catcgttccc cccgggattt tatgcgccgc tccaaggagg ggaaaaggcc
    23161 cagatgacag ttaatgggtt gaaagtgatc cggaggggat tcctggagat aggccaaaag
    23221 cccccccagg gtcgtctccc ggggccaggg ggagggaagg gtcccagaga gcagggtcgc
    23281 caggtggatc gccgccacca gtcccatggc ggtgctctcc acatagccct ccacgccaca
    23341 gagctggccc gccaagaagc agttccccga cccccggagg cgcagcaggg ggtcgagcac
    23401 ctcgggggcc cggacgaagc tattgcggtg catgaccccc catcggacga actccgcccg
    23461 ctccagccct ggaatcagtc gaaagacccg ttcctgctct ccccaccgga ggttggtctg
    23521 gaagcccacc aggttgtaca gcgtgccttc ccggttttcc tggcggagct gaaccacggc
    23581 gtggggctct cgcccggtgc ggggatcggg gagccccacg gggcgcatgg ggccaaagcg
    23641 tagcgtgtcc cccccccgag aggcgatgac ttccacaggc aggcatccct cgaagtactc
    23701 cgccttctgg tccaccgagt gcagcggagc ccgttccgcc ccccgcagag cttccacgaa
    23761 gcgctggtac acggcggcat ccagggggca gttgaggtag tcgtctccct tgccccaccg
    23821 gctggcgcga aagaccacat cgcggtcgag gctctcggcg gtcaccacgg gggaaatggc
    23881 gtcgtagaag gagaggaatc cccccgtgcg tcgctttagg tcttccgcca ggcgcccctc
    23941 cgtgagggga cccgtggcca cgatgcaggg ctcggggggc agggcctcgc actcttcccg
    24001 gcatatttcc acccgggggt gggtcgagag cacggcggtg attctccggg aaaagagctc
    24061 ccggtccacc gccagcgccc ggcccgctgg cacggcagtg gcgtcggcgc aggccaggat
    24121 gagactcccc aggcgccgca attccgcctt gaggattcct cccgggctgg aaagttcatt
    24181 cgcccccagg gaattgctgc acaccagctc tcccagggcc tccgagcgat gggccgggga
    24241 gggcacgccg gggcgctttt ccaccagctt caccccatat cctcggcggg cgagctgcca
    24301 tgcggcttcg ctccccgcca gcccccctcc caccaccacg atggaggggg aatgggtggc
    24361 agtcatgccc cttcccccga ggctcccgca acacggcggc gcgacggcgc cttgggggct
    24421 tcggcgtcgg aatcgaggtc ctcggggact tccaagggcg ccttctttcg ggtcggggcc
    24481 tttttagggg tggcggggac cttcccatct tccagaaggg ctcccgcaac acggcggcgc
    24541 gacggcgcct tgggggcttc ggcgtcggaa tcgnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24661 nnnnnnnnnn nnntttcggg tcggggcctt tttaggggtg gcggggacct tcccatcttc
    24721 cagaagggat cccgcaacac ggcgaaccgc cttctttttt gactccccgg cggcctcgga
    24781 ttttttggcg ccccggccct tctccggcgc caacggtggg gtgcggaggc cttcttctcc
    24841 ggagtcgctc gccgcctccc ctcgcacttt tatcccggcg caagcggggc agaagggggt
    24901 cttgcttcgc ccccgcaccg ccaggaagcc gccacaggtc gcgcagctcc cccccgcagg
    24961 ttggagccag gacacgaagt cacaggaggg gtagcgggag cagccgtaga atacgcgccc
    25021 ccccttgcgg ctccggcgcc gaaccacgtc tcccgtctgg caggtggggc aggatacgcc
    25081 gatgcttttc agcaagggac ggctgaagcg gcactgggga tagccggaac aggcgatgaa
    25141 ctctccgaag cgcccccgtt ttatgacgag aggagatccg cactccggac attgttcccc
    25201 gatctcccgc acggggagca tcacccgctg ggcgtttttt tccgcctctc tcaactggga
    25261 ggaaaaggga ccccagaagc cctccaccac ccctcgccag gggcgccgcc ccccttccac
    25321 gtcatccagc tcgtcttcca tgcctgcggt gaattcggtg ttgacgatgc caggaaaatg
    25381 ggccacgaga aaatcgctca ccgccagccc caagccggtg ggctggagac gcttttcctc
    25441 gtttcgctcc acataccccc ggtcgtagag ggtctggatg atggcggcgt aggtggaggg
    25501 gcggccaatg cccttttcct ccagggcctt caccacgccg gattcggcga agcggggagg
    25561 aggagtggta aagcgctggg ccgtagagac ggagcgcact tccagcgtct ctccctcccg
    25621 ggcctggggc acgctctctt ctttcagatc caagggccag accttgcccc agccgtcgaa
    25681 gaggagccgg gccccggtgt gcttcaggcc gtagatgtcg cacgtcgcct ccacggtggt
    25741 gctggccacg atggcggcgg ccatctggca cgccacgaaa cggcgccaga tgaggtcgta
    25801 gagcttgtac tgctcgccgc tgaggctttt tttcagctcc tcgggggtga gggttacgtc
    25861 ggtgacgcgg atggcctcgt gggcatcctg ggatcgcccc ttggccacat atgccatggc
    25921 caccggggga acatagctct ccccatgggt cttttcgatg aagcgccggg ccatggccac
    25981 ggcgtcaccg ctgaggcgca ggctatcggt gcgcatgtag gttatgagcc ccaccggtcc
    26041 tcggccggga agctccaccc cctcgtagag ctgctgggcc accgtcatgg tcttccgggg
    26101 ggcaaagccc aggcgccggg cggcctcttg ctgaagggtg ctggttttaa agggcggcag
    26161 gggagggcga cggctctctt tttcccggaa ggaagacacc gtcagcgcct ctcggcgcag
    26221 gacggcctcc gcgctccggg cctcttcttc gctctccggg gcccacccct tgcccgagcg
    26281 cttctccagc ctcagggtaa aggatcgccc cccatcgcct tcggcctcca cgtccaccaa
    26341 ccagtactcc cgggggacga agccttcgat ctctcgctcc cgctccgcca gaaggcgaag
    26401 agccacggac tgcacccggc cagcggaaag gcggggctga atttttttcc acagcaaggg
    26461 ggagagggag taccccatga tgcgatccag caccctgcgg gcctgctggg cctccacccg
    26521 actggcatcg atgggttccg gggccgccac ggcctctttt acgcccttgg gggtaatctc
    26581 gtacatgcga atgcgacagg atgttcggcc gtccaccccc agggcctggg ccaagtgcca
    26641 ggcgatggcc tctccttccc gatcggggtc cgaggccaga aggacccgat cgcttttggc
    26701 ggcctgggcc ttgagctctt ttaggatttt tgccttgccc ttgacgggga tatactgggg
    26761 ctcgaaaccg ttttcgatgt cgatgccgag cttgctccgg ggaaggtcca tgacatgccc
    26821 catgctggcc gcaatggaaa agccccgacc caggatcttt cccagggtcc gggctttggc
    26881 gggggattct acgatgacta aggtcttttc cgccatggcg gcctcaggaa gcccaacgag
    26941 tggcggatag gtagaccccg tccccctctt cgtcccacgg ggcccttccc ggcatggggg
    27001 cctccccgca ggaaaagagc gtcctgaagg cgtcgactcc tacggggacc ccttcggcct
    27061 gagacagaac catcgccccc tccagggatc gttccagcgc cgaatccgaa tggggctgga
    27121 ttcccagata aaccgccaca aggcgccaag tttcgggagt gagagccgaa cactcccggg
    27181 gatgcaaaat ccttatggac acgcgcgtcg cacctccgga tggtctgttg ttgagcttta
    27241 ttttttaagc gaggccctcc accggccagg gcccgatggg tataccaacc ctcggacgtt
    27301 gagcagggcc aaggcactgg aaatatgggc ggagctcatt gtaccccccg tggcaaggtt
    27361 gtcaatcgtc tgatctcccc ggtcgcgcaa ctgggccaga atttttcgct ccaaatcccc
    27421 caaatcggac ggcaaatcgt atttttcatc cccatatagg cccagcgtcc cctgaagggg
    27481 ttgccctccc agggtttcgg cgaagtgcgc cacgtccacc agggggaatc ctccatccgc
    27541 caacagggcg ttggagccct cgcagacgtt ttcgtcgatg cgcccgggca ccacccagag
    27601 ctctcgcccc atctccatgg cgcggcgcgc ggtgaccagg gcgccgctgc gccccggggc
    27661 ttccaccacc accgtacggg atgccagggc cgcgatgata cggtttctct ggggaaattg
    27721 ccacgggcgc cccgtcgttc ccagggggaa ttcgctcagc aacgtccccc cctgggccac
    27781 gatgcgccga aagaggtccc cgtgctcggc gggaaaaaag acatccaccc ccgtgccaaa
    27841 cactgccgcc gtggggcctc cggattccag ggcaccgcca tgggcgcctc catccacccc
    27901 catggcaccg ccgctgatga gccagcgccc ggcccgggcc acccggcgtc ccacctcctg
    27961 ggccacctga aaggcataag aggtacaccg ccgagtgccc accaccgcca cccctccgga
    28021 gtccaggggc cactttcccc ggacgtatag cgccggagga gggtcggcca ggtcccacag
    28081 ggcgggagga taggaagggt ccgccgccgt caccaggtgt acgccctgga aggcggcccg
    28141 ctccgcttct ttctcggcaa aacacccctc cgccaagccg cgaaagcgac gcaccgtccc
    28201 cggaggcacc cccagggcgg tccaaagggc gtcgctcccg ggctccacaa gctcttgcca
    28261 ggaggatccc cgggaggaga gctcctgcca aatccgggca ttggtcgccc ccgccgcccc
    28321 cagggtaaga agaaaaaggg ctccttccgc catgccgtcc atgccgtatt cccccctcac
    28381 cacagtgccc cgggacgata gcccagagct tccgccagat gggacactcc tagcggagcg
    28441 ccgtcgccgt cgaggtcggc gatggtccgg gccaccctca acagtcgact cagtccccgt
    28501 cccgacaggg tcatccgccg ggccgcatcc cgcaaaaagg ccgcttctgt gggccccagg
    28561 ggggtctctc ggcgcaacag gcgctcggga atttcggcgt tacagcgata tccaaagggc
    28621 tcccaacgac gcctctggat ctcccgggcc cgaatcaccc gggacctcac ccgggcgctg
    28681 gactcccccc ggcgatctcc caggcccacg agctcttccg gcgagaggcg aggcaccgcc
    28741 acgtggaggt cgatgcggtc caggatgggg ccggaaaggc gccgacggta gcgctctcgc
    28801 tcctgggggg cgcaacgaca gggctggatg acgtcgcccc agaagccgca cgggcagggg
    28861 ttcgccgccc ccaccaaaag cacacgagag gggtaggtga cgctccccgt ggcccgactc
    28921 acggtgatgg tggcgtcttc caggggctga cgaagggcct ccagcagatc ccgtcggaac
    28981 tccgccagct cgtcgaggaa gagcaccccc cgatgggcca gactgacctc gccggggcgc
    29041 agcgccgatc ctccaccgca gaccgccgcc gtgctggccg tatgatggac ggcccgaaaa
    29101 gggggctgaa ggtcggggat ctcttcctgg ccgtcggcgc tcctcacccg gaggacctcg
    29161 agaaactctt cggaggacaa gggaggcaag atgccccgca gggcccgggc caaaagggtc
    29221 ttccccgacc ccggagcccc cacgaaaaat aagttatgat gccctgcggc ggcgatctcc
    29281 agggctcgcc gggccccgga ctgccccttg acgtcttcca ggtccgtatc gacccggggc
    29341 tctccgggac tcagggtgcg cctctccaag gggctaagtg gcgccccccc tcgcaaatgc
    29401 tccataagag aggggagaga gtccaccgca aaggcctcca ccccatctac caacgccacc
    29461 tcggaggagt tttcccgggg aaagaacagg ggaaggccca tctggcgcgc cagaagggcc
    29521 gccggcacgg ccccccgacc ccggcggaga cgcccatcca gcgccacctc tcctaggtaa
    29581 agtgccggag gaggggaagg cagggccccc gacgccaccg ccatgcccag ggccatggga
    29641 agatccagca gggccccctc cttggggatg tccgctggcg ccaggttcac cgccacgcgc
    29701 ccccgcaaag ggatccccga ggagcgcagc gccgcccgca ctctctcccg ggcctctcgc
    29761 accgccgcat ccggaaggcc caccacggag atgacaaaaa gccccccggt gatctccacc
    29821 tcgacttcca cgggcaccgc atccacgccc cgcagcgtta ccccccgcac gacccccaag
    29881 ggtcccaccc ccctgcggtg atgtcttcca gatgttccct ccgccacacg cccttccgat
    29941 ccaccgtgaa agccaccagg tcgatgcgcc agggaccctc ccaatgccag cgctcggcga
    30001 ggatccgggc tccccggacg aggtgggcca ccttccgggg ccccacagtg gtctccgggg
    30061 gttgccactt tcccagggag cgcacccgta cctccacgaa gaccatatct ttccccatcc
    30121 ggcatgccag gtcgatctcg ctctttccgg cccgcaaatt cctccccagg atggtccatc
    30181 ccagggaacg gaggtaccgg gcggcctcct cctctccctg acggcccaag tcggcggggg
    30241 aaggggccga agggatttga tccccgggcc ccgtcgggag ggacccttcg tcatccttga
    30301 ccttccgggc atctttcacg acctcactcc tcgccgttcc ttgtccagac ggtacacaaa
    30361 ggataacacc ttggccaccg cctcgtagag ttccgtcggc acctcgtccc caatttccag
    30421 caccaacagg gcgctcacca aggccgcatc ctccacgatg gggatatggg cttccagggc
    30481 caggtcccgg atgcgctccg ccaccacccc tttccccgac gccaccaccc ggggggcccg
    30541 atcgcgagat cggtcgtact gtagcgccgc cgctttaaat cgttcggtcc ggggtttttc
    30601 tgccacggct caggcctcta gatccacccg acgaagcctc gtcggggttc ccccggggcg
    30661 gggcttcgtc acccccgccc ggacgaggcg caacgcccca tcggccaggt cgcgctccag
    30721 ctccggaagg cgccgccgca gcaaagaagc catcatgggg tcttcggggc gcaagttcac
    30781 cgccagctgc acgccgtcgc tggtgacctc tccccccacc acccccagag cctcgccctc
    30841 cacctcgaag gccacccgat agaccttccc gtgcttggtc tcttccacct gggccgagac
    30901 cttggccgga aggggctctt cgtcgtccaa gggccagcgc accggagtca gcagggaggg
    30961 gtcgactccc tcccgggccg gggtggctaa aagtcccgtg gcggtggcga agcgccgtag
    31021 ctcgtcgtct tccagacgcc cccggagata gccttcccgg ggagtcgcgg ggtctttgag
    31081 ctgatcccgg atacgccgcc agtggcggcg cacctccgag ttatccagcg ttgcatacca
    31141 agccaagatg cgaaggtttt cctcggtgag gggaaggtgg cgggcccaga gctccaccat
    31201 gggccccagg cgttccgatc ccccccccag gcgcaaccac gctccccgga gggaggagag
    31261 gacatctttg gagaggggaa gaccccggga aaggagggcc tccgccagat cccggtcttg
    31321 gggcgaaatg cgccccagca gggcctggtc gctctcttga aggcgcagca tggggatgtc
    31381 cccagaggcg tcccacacgg cgcgaaagcg ctgccccacc gagaggggca cggtggatcg
    31441 ggcggtgaga aagcgccccg ccacctggat ccggtaggcc tcttccccct ccagggccac
    31501 cacggtgccc tccaccaggg cgccgtggac caggggaggc gccccatcgc gcccccgccc
    31561 cccgtcgatg ggacggggaa agccctcggt cccccgggga ggaatacccg aagggggcag
    31621 atcgccgatg ccccgcacca cctaggatcc ctccccccag gaaaagctgc gccggtgcac
    31681 gggagagggg ccgtaaaggg ccagggccct ccggtgggcc tccgtgggat agcccttgtg
    31741 ggccccgaag ccgtactcgg ggaagagacg acctaagcgc tccatgatcc ggtccctcag
    31801 caccttcgcc accaccgagg ccgccgcgac gcagagcacc cgatcatccc cccggggaag
    31861 ggcacgttgg ggcaggggta acccagggat ctccacagtg ccgtccacca ccaccaggtg
    31921 ggggcgctcg ggga
//
LOCUS       DAOOER010000020        31706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_1622_length_31706_cov_9.359578, whole genome shotgun sequence.
ACCESSION   DAOOER010000020 DAOOER010000000
VERSION     DAOOER010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 31706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31706
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1622_length_31706_cov_9.359578"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="PL162_04355"
     CDS             complement(<1..823)
                     /locus_tag="PL162_04355"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQF91284.1"
                     /translation="MRCALFFVGGILGFLLLGGNPGEARGISFDFGPMDVLVRQEMAS
                     GGIPAVAVGLLLPDGQTLSRVYGSADVEQDVPAAADTLFAMGSVSKVFTALGVLKYLE
                     EDRITLEDPVSAYFPGYPEGERISLCQLLQHTSGVAEVTKIPAWSADPARDWTPQEAL
                     ALFSKEPLEFEPGQRAQYSNSPFIILGILLERLSGRSYERFVREDLAAPLGMTTLRLG
                     SQRDIMPHRARGYALEGGRLINAPPESPVSAYASGGLMATAEDLAKLGKAFLPGVL"
     gene            complement(1008..1898)
                     /locus_tag="PL162_04360"
     CDS             complement(1008..1898)
                     /locus_tag="PL162_04360"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQF91285.1"
                     /translation="MRRSLVGVLLLFAMFFAVPAGAQDSLALHYAPAGEFAVAYRDLG
                     SGDRPLVLVHGWACDHTFWRYQIPFLARRGRVIALDLPGHGWSTKPKISYTPEVLVEG
                     LRAVMDDAGVSQGVLVGHSMGGALCRAFAQRYPHRVAGLVLADPALVRAPADPQKREE
                     FVQMMKGWATSLTTGPEARDHVRAFIEGMFVPETPETVRRLVLDKMLGTAPYVRDSAM
                     VNFLHLPTREESLPTYVPTLALFGAETVRAEGEGLEEELRRLFPHLTYEVWPGVSHFY
                     MLEVPDRLNDRLAAFAASLP"
     gene            2246..3133
                     /gene="pdxS"
                     /locus_tag="PL162_04365"
     CDS             2246..3133
                     /gene="pdxS"
                     /locus_tag="PL162_04365"
                     /inference="COORDINATES: protein motif:HMM:NF003215.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HQF91286.1"
                     /translation="MEERSNPKNPRRDMTWMKPRGVIMDVTNASQARIAEKAGAMAVM
                     ALERIPSEIRKAGGVVRMADPDKIKEIQDAVSIPVMAKARIGHFVEARILEALGVDCI
                     DESEVLTPADEEHHIDKRPFAVPFVCGSRNLGEALRRVAEGAAMIRTKGEAGTGNVVE
                     AVRHMRATNREMARLLGAPEEELPALAKELQAPLETLWECRKRGRMPVAHLAAGGVAT
                     PADAALMMQMGCDGVFVGSGIFASSAPERRAEAVVAAVAHYDDYAALAEVSRGLGEAM
                     PGIDLRTLSPEETLAVRGW"
     gene            complement(3208..3960)
                     /locus_tag="PL162_04370"
     CDS             complement(3208..3960)
                     /locus_tag="PL162_04370"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HQF91287.1"
                     /translation="MAPHIGITCDLDDQRTTNTLPRAYVRAVMRAGGIPRVLPIPEME
                     HTPGGFTPGNGLGEDWGEWLSGLDGLLLSGGGDLDPALFGEEPLPGLGGVDLHRDALE
                     MALARAARRRKMPLLGICRGCQVLNVALGGTIYQDLPSQRPEGLLQHRQPYPGTFPSH
                     SMTVREGTRLAHVLGSLSVRVNSFHHQAVRDVAPGLEAVAWAPDGVVEGIEAPEGPFL
                     LGIQSHPERLESTSAPMAALFRAFVAAAGEKK"
     gene            4186..5502
                     /locus_tag="PL162_04375"
     CDS             4186..5502
                     /locus_tag="PL162_04375"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HQF91288.1"
                     /translation="MALVQVVEWDEPVTREVPVLAWKYTNPAQPERSDELGTWTQLVV
                     RESQEAILFKNGQALDLFAPGRHTLGTANVPLLAGLLRLPFGGESPFKAEVWFVNKVS
                     MLNVKWGTPDPIQIQDPRYKVFLPVRSFGQFGVRVADARKFLVKLVGTVRLFDQEHLN
                     TLFRGLVLTKAKDLISSYLVKQGISVVEVNAHLDDMSGHIRERLASVVEEYGIEVPNF
                     FVNSINIPEDDPAVAELKKALAEKARMDIVGYNYQQMRTFDTLQAAASNEGSSGTLLG
                     AGLGLGMGVGMGGAFGGAAASMAGSLSPTGATPCPRCGALNPPGARFCLSCGALLENS
                     VPALCSRCGGSLPRGAKFCPACGDRYDPCPTCGADLPEGASACPKCGAPRPLPCPRCG
                     ASSPPGTKFCPGCGQTLTPGPLKCAACGAEMSPGAKFCPECGARRD"
     gene            5543..6223
                     /locus_tag="PL162_04380"
     CDS             5543..6223
                     /locus_tag="PL162_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91289.1"
                     /translation="MRKFSLSLLILLALAVLAVGTGVILYLSTTPEDRHLIFWISLGT
                     VALGAALSTLGALINLWSDSDRALPFPSTLSLTAVTGGYTLFALGAAVAAWRFVDFSI
                     TGYIITHVAGGTIFLVGGIFALMGGVQTSSEDRRAARGRAWLADMAREVELLTATRPG
                     DSAVACRKLAEALRFSDPVTPKRFAEEDRAVERAVARVRESGASPESVQEALATLKRR
                     NDLVARGK"
     gene            6278..7003
                     /locus_tag="PL162_04385"
     CDS             6278..7003
                     /locus_tag="PL162_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91290.1"
                     /translation="MSDFSSDRQLNPPFYLSWPVIIISFIFWPVGLVLLFARLGRDRH
                     AGITTGGVLKVVGVLLFLVCLLGGYGIYENEMTSFGDLTDDGYASLAVAGAFGVLGII
                     LWRYGRTLQYRGRMVREYLGLIVNRRFSNLDDIARIVGKSRPQTEKEIDFLIEKGYLP
                     EALVDRGAGQVIVPSVLRHMEAESRWKSSQEGENPAPRENAPAGPQVVACRSCGAQGM
                     IFPGRPAVCEYCGSALKVSGGRQ"
     gene            7050..8012
                     /locus_tag="PL162_04390"
     CDS             7050..8012
                     /locus_tag="PL162_04390"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HQF91291.1"
                     /translation="MKPKVFVTRQIPEAGLAILRDRTDLEVWPHGDIPVPREVLADRM
                     PHLHGVLTLLSDPIDRGIIDRGPHLKVISNYAVGTDNIDVAFAQARGITVTNTPDVLT
                     DATADLAFALILGTARKVLEGNQVARSGRWAWAPDLLAGEDVFGATLGIVGFGRIGRA
                     VARRAQGFSMTILVAARDSAARDAAELGAQMVPFEELLEKSDYVTLHCPLTPETAGLM
                     NEEAFRRMKKTAIFINTGRGKLVDQRALGQALSEGWIAGAGLDVFDPEPLPADHPLFA
                     APNLLPLPHLGSATRRTRERMATMAAEDLVAVLDGKIPCHPVRI"
     gene            8021..9331
                     /locus_tag="PL162_04395"
     CDS             8021..9331
                     /locus_tag="PL162_04395"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HQF91292.1"
                     /translation="MGSSLGDTVRAIALEALATCSPEDAVRRVLQPIPSGARVGDRQW
                     DGPFYVLSVGKAASAMARAASDLLGNLVWAGSVLTRDGYSDEIPFMNCLEAGHPLPDA
                     RSLNATADILREIDALPDDVTILMLLSGGGSALFEAPDKGISLEDIRAVNAELLTCGA
                     SIAEVNAVRKHLSRVKGGKLLRRISPRSSLALVVSDVVGDDLATISSGPTVADHTTFA
                     DAVEVLKRRDLWGKTPKNVRHVLEEGLKGLREETLKSVEGYDTWAQVILDNRSLCRAA
                     GEAAKIRGFTPLFLGSFFSGEAREIGTFVGEIAREIRASKHPGPPPVAVISGGESVVK
                     VRGKGRGGPGQEAALACARALRGLSGVAFLAMDSDGTDGPTDAAGGVVDGTTWDHMES
                     RGLNPLDALERNDAYAVLGSGGGLWKTGPTGNNLNDLRILLVEE"
     gene            9390..9800
                     /locus_tag="PL162_04400"
     CDS             9390..9800
                     /locus_tag="PL162_04400"
                     /inference="COORDINATES: protein motif:HMM:NF024210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91293.1"
                     /translation="MGRKVLRVIHRDRCIGCFSCMYACSRTFAGMATTNRAALRVRAY
                     TGVEGAFSLRTCLRCPEPDCAAACPSGALKKVEGGGVRLDKERCLSCGACVQACVIRA
                     LQWNADDRRPIPCVHCGTCARYCPNGVLALEEEA"
     gene            9815..11560
                     /locus_tag="PL162_04405"
     CDS             9815..11560
                     /locus_tag="PL162_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HQF91294.1"
                     /translation="MQETILHIDLTTGTARRERRPELFDRFLGGTTLGTELLWQYAPP
                     EVDPLGEQSALIFVTGPFSSVFPVATKTVALFKSPLNGELGETHAGGRLAMALRDAAI
                     DALVITGKSPSPTFLSIENHEVTFSSARTVWGQSALASERILREYAAPDGRKGSVVRI
                     GPAGERLAPLACATVDGGRHFGRLGLGAVMGSKNLKAFLVSGTGGPPIIHRGEHRQVY
                     DAIYDRVVASNDMKKYHDLGTAGNILPLNAIGGLPTRNFSQGFFEGAQALSGEAFAEH
                     YLVQHMACAHCPCGCIHMAALREPFAPFHYRTTKIPYDYELIYALGTLLSLSSPPEVL
                     SLVDVVEKEGWDAMGMGTALAWASEAFVSGVVGLRETEGLPLRFGDGGGYLQAMHRIR
                     KGEGEFFRDLERGVAFCAAKYGGEAFAIHFGGVGAPGYLTGENALLTYLMGVRHSHLD
                     DPGYGTDQKLLGKSLSLEEQVGAAVKDAQWRMVTNSLVTCLFARGVYDEEMVLAGLGA
                     LGLSWDGEKLKRMGEEALRRKYAYKIACGADPRRGKIPEKIFSGTTATGPVDRGRMEL
                     RRELYARMVGLPEEV"
     gene            complement(11597..12121)
                     /locus_tag="PL162_04410"
     CDS             complement(11597..12121)
                     /locus_tag="PL162_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91295.1"
                     /translation="MGGKAAVKALVLAVAWWALGASSAPGFSSSWAVYTNPRFGYTVD
                     VPADFPAGEAPENDDGRTFTPPGRQGSVAVYGSFNALGHSPEDHREWLKEGFANAGGR
                     ARVTYEATGKNWCVLSGFTAEGAIFYQRVRFSADGDTIIAVHFVYPEEDKEEWDPVVE
                     RMGKTLRFVDAEGN"
     gene            12503..13453
                     /locus_tag="PL162_04415"
     CDS             12503..13453
                     /locus_tag="PL162_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HQF91296.1"
                     /translation="MRRSFVRWMAAMVVLGVMVSGASATVFINIGTGSTGGTYYPVGA
                     AMAKIWNEKIEGMKANAQSTGGTVNNLQLMTDNEAEVGFMDGAYYAAYTGTGKFEGKP
                     MENLRALVPLYPEPVQLMVARQSGIKTLGEFKGKKVSIGAVASGTEVTARQLLKVAGM
                     DPDKDITPENLGVGDTTGAFGDQRIDAAVMVGALGMAGVVEATTLGLVDFIDVPQDIL
                     EKVMKETPYWVPFTIPANYYKGQPNEVKTYASWNILGVKADLDEELAYRMTKALFENR
                     ADLVLVNSKMENMAPENVKFIMIPLHPGAEKYYKEIGAVK"
     gene            13530..15503
                     /locus_tag="PL162_04420"
     CDS             13530..15503
                     /locus_tag="PL162_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HQF91297.1"
                     /translation="MAEESRENLIEKNSLGEKEIQHLVEQYDVESRFRRLSGFAGKFA
                     VCWCAAMSCFHLYTAGIGLLPTSIQRAVHLLFAMVAVFLLYPSHQRVDKTRIPWYDWI
                     LALLAAIGTGYIVFFFNDIAYRGAKPLPVDIVLGYMTLALVVEAGRRILGNVLPMLAV
                     FFLLYCFFGRYAPAMFMHRGYSVARVIQHMYLTSEGIFGVALGVSATFVFMFVLFGAF
                     LAKSGGARLFNELALALAGHSPGGPAKVAVVASGLLGTINGSSVANVATTGAFTIPLM
                     KRVGYPPEYAGAVEACASTGGQLMPPIMGAGAFIMSELLGVPYLSIAAAAALPALLYY
                     SAIFINVHLWARALGLVGLDRASLPRPRDVLRRDGHLLVPLGAIIAMLLMKFTPLASA
                     FWGIVAVVLVGALRSSSRLGWREILAALDEGARGSLGVAMACALVGFVVGASSLTSLG
                     LNLSNNIIHLSGGNLFFMLVLSMIACLVLGMGLPTTANYIVCSTIIAPALVKLGALPL
                     AAHLFVFYFGIMADLTPPVCLAAFTGAGIAGADPTRTGIQAATMALVAYLLPYSFVYT
                     PMILLQDVAPLPLGILVVASVLGVLGLASAGRGWMFRTLGGVERIVALALGVGAFWPH
                     PVVKIAAALGLVSFLFLERLAARRAAERSVQGA"
     gene            complement(15772..16503)
                     /locus_tag="PL162_04425"
     CDS             complement(15772..16503)
                     /locus_tag="PL162_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HQF91298.1"
                     /translation="MTAPFFQGQDLAFSYPDAPGALEGVSLDLAPGEALGIVGANGAG
                     KSTLLLLLAGVLPPSRGRFVLDGAKIPTPFPPSVRRRLGVVFQDPDDQLFLPTVYEDV
                     AFGPRHRGLSPQEVHAAAWGALERVGARHLAPRMPHALSGGEKRLAALAAALAPAPDV
                     LLLDEPSAALDPRARRHLIELLRSLPEARIVASHDLDLVLDVCPRVMILREGHLFAQG
                     ATADLLYDAPLLDAAGLELPLSHRP"
     gene            complement(16500..17300)
                     /gene="cbiQ"
                     /locus_tag="PL162_04430"
     CDS             complement(16500..17300)
                     /gene="cbiQ"
                     /locus_tag="PL162_04430"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HQF91299.1"
                     /translation="MTSLEHRLRAASHTLDFGRRLSPIHDLDPRTKIAVTALFLVVTA
                     SFPSSAPGDLAPLLFFPCVVGALAGIPGRALLGRALVLSPLVLLVGVAEPLWHRTPVI
                     IAPGIALAAGWFSLLGIVMKFVLSVSGALLLLRTTPLDDLCSGLVRLKVPRVLALQIL
                     LLVRYLFLLGDEALRTLRAHGARTGGQSPSLALAGPLLGSLLLRTLARGERISRAMAA
                     REFGMGALEGAEAPLDRRNLLFLAGWGVFFLLVRLRPLSRLAEIGGLS"
     gene            complement(17297..18325)
                     /locus_tag="PL162_04435"
     CDS             complement(17297..18325)
                     /locus_tag="PL162_04435"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HQF91300.1"
                     /translation="MHMADALLSPAVGGALWAVSAGLVGYAARKLDQDPAPSRAPLMG
                     ALGAFVFAAQMINFTIPGTGSSGHLGGGLLLAALLGPHAAFLVLTSVLAVQALFFADG
                     GLLALGCNIFNMGFFSCFVAYPLIFRPMTGENASSRRILGASLAASIVGLALGALAVV
                     LETGASGISSLPFGTFVLLMLPIHLAIGVGEGFITASVVLFARRVQGIAPERSVSPGK
                     PLVMALVVLALLTGGVLSWFASTHPDGLEWSLFHATGQEEVEGPATSVHTALANVQER
                     TALLPDYALPASDISPESSAPEGDASWPAVSGGTSLAGIAGGLLTGLLALALGWGLRR
                     RQACRASS"
     gene            complement(18631..20361)
                     /locus_tag="PL162_04440"
     CDS             complement(18631..20361)
                     /locus_tag="PL162_04440"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQF91301.1"
                     /translation="MKNSLVSKLAALIVLGILVLAAVLLFLARSGSQDMLRATSGVAD
                     RMLTEDIQNKNQANQESAATYGASMAQYLAFISASPLWNLQEELLKEYAQGMLQLPNL
                     AYAVVYDDGNKAVAGEKKEGGAGVQVFTHGVVYDGKSIGQVEVGLATDHLEAALKKNE
                     QVKTDLISTFEKEAQGAQSALVSRLGITAAAIAVVLLVVSLLVLFRIAKPLRRMVGVV
                     QDLGQGEGDLTVRLDVASHDEVGLLGESLNLFVDKLAAMVRELVEVAGEVGAQARSLA
                     ELSERSTVVADRSRDAVDQIVVLSESNAAAVQETNAGIEEVASSASASAKSAEAGAHS
                     STKSTHLTEEVVSRMAGVIQSIVTVGEQAHANRHTIQTLSGSVTDITNFVGAITSIAD
                     QTNLLALNAAIEAARAGEAGRGFAVVAEEVRKLAEESNHAAKEIGGIITTLQKSAADS
                     IKATEASEGTLNEVVKAAQGVQGQLQESLREVVGVNDVMQNIAAVAEEQSASSHEMAT
                     AIDSITHGASAIVESLGTIRASTEETADSFQEVARNAAHLSENVAHLEQILSQFRIDQ
                     SEERRALPRA"
     gene            complement(20426..21193)
                     /locus_tag="PL162_04445"
     CDS             complement(20426..21193)
                     /locus_tag="PL162_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HQF91302.1"
                     /translation="MKTRFFLPLTVALVLISFGTVALADDIKSVSVILDPWPPYSVGE
                     TNKPPTGGYFVDVCQEAFSRLGVEANIMLYPWKRCLKSIQEGDSDILLGVTKTAEREK
                     FMTYSDPVVVDRVVFFYVKEKYPNGFTWENYGDLKDLAIGTTAGYNYGEEFNAGVKEQ
                     GIKLDEGESETKSFEKLAAGRMDLFVCYEISGKYWVNAHDDLRGKIDVAEKPVLAEMP
                     FYLAFSNKGKALPLVPKLNEVLAAMKADGTTDKLLGK"
     gene            21773..22435
                     /locus_tag="PL162_04450"
     CDS             21773..22435
                     /locus_tag="PL162_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91303.1"
                     /translation="MTGFFRRFLKLIFGLFLYAVGIVLTINAHVGYGPWDVFHVGLGR
                     ALGITLGTASIITGFVIVAATYLMKETLGIGTILNMFLIGIFIDVIMHGELIPVMDRT
                     IPGVIMLLAGMYVIALATYFYIGSGFGTGPRDGLMVALTRRSRLPVGLVRGLIEVSAI
                     AFGWFLGGMVGIGTVIYGLLIGFCIQSTFRLLKFDPTKISHASILESCRELRVALGDR
                     GA"
     gene            22508..23338
                     /locus_tag="PL162_04455"
     CDS             22508..23338
                     /locus_tag="PL162_04455"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HQF91304.1"
                     /translation="MIDVVLSPGETLPRVSSWLVVDLLRATTAMTAFFEAGGEELLPV
                     ATVEEARSLREGLGTAWLLLGEQGGERPEGFDYGNSPREIRASPLQGRRGIVATTNGT
                     GALLRAAEAGGAVGAACARNADAAAHFAARRGGGVAVLCAGLHGRLGLDDVFCAGLLV
                     ERLRDLTGQDLGDGARLALMAWEKVRPDLLGAVRRTVHAGRLALKGFDEDVADGCQMA
                     VSSVMPVLVRHGGRLVLVREEKDRAEVAEDLGIGTPDRRRKGCRPGGTPSEGRKCLGR
                     "
     gene            23343..23537
                     /locus_tag="PL162_04460"
     CDS             23343..23537
                     /locus_tag="PL162_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91305.1"
                     /translation="MNRGGFSWKRFLGISAAKSRISRKIGIPLTRSGRQRKVGAALGC
                     VWPLAGVMLLAVAATVSFFL"
     gene            complement(23548..24426)
                     /locus_tag="PL162_04465"
     CDS             complement(23548..24426)
                     /locus_tag="PL162_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQF91306.1"
                     /translation="MTPDRPPISPLIPLILGIGAISTGAIFARMADAPSLVIAAYRVG
                     LATLVLAPFALARRWGELRNLPRRDLVTVGGAGFFLALHFATWIASLNYTSVASSVVL
                     VNTIPLWVGLLGPLVAGERFSRWTAGGMALAFAGGAVVGGGDFALGGQALWGDLLALA
                     GALAAAAYLLLGRRVRPKLSLLSYIFLCYGTAAAFLWAGVLLTGLPVAGYSSGTWGAF
                     GAMALIPQLLGHSSYNWALRWLSAGFISVALLGEPLGSTLLAWILFGEGLTLVKAAGM
                     GGILAGIVLASRGEGA"
     gene            complement(24592..25503)
                     /locus_tag="PL162_04470"
     CDS             complement(24592..25503)
                     /locus_tag="PL162_04470"
                     /inference="COORDINATES: protein motif:HMM:NF015056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HQF91307.1"
                     /translation="MSAVTYSVDSSGLVYSFSRHHPPRLRVPDGSRLEIETCDCFENQ
                     IRRPEDTLASLDWDRINPATGPIFVEGAEPGDVLAATIEAITMDDQGVLVAGHDFGVL
                     ASKFDALHSRLVPVRRGRVIFDDRLNLPVRPMIGVIGVAPGGDPVNCGTPGSHGGNMD
                     TTLIAPGATVYFPVAVPGALFALGDLHAAMGDGEISGAGVEIAGRVTLRLSVRKDLSL
                     AGPMVATPEGLAAIASAPTLDDAANQAVRELAEVLFPRLGLPWQELAYLLSIAGSLQI
                     CQVVDPLKTARFVLPWSALAPYGVTEL"
     regulatory      25834..25958
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            26072..28801
                     /locus_tag="PL162_04475"
     CDS             26072..28801
                     /locus_tag="PL162_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQF91308.1"
                     /translation="MIQKRFFVNGVERNLIVDGESSLADVLRGQLGLTGTKVGCGTGE
                     CGACNVILNGKLVRSCVYKMKRVEDGDQITTIEGIGTPDELHPLQRAWVSSGATQCGF
                     CSPGFIVSSKVLLDHNPNPTREEVREWFHQHRNACRCTGYIPQVDAVMAAAQVLRGEA
                     PEESLDFKMPPDGRIWGSAYPRPTAVGKATGTLDYGADLGLKLPTNALQLALVQATVS
                     HANIKGVDVSEAEKMPGVYKVVTARDVKGKNRITGLITFPTNKGDGWDRPILCDEKVF
                     QFGDAIAIVCADTYENACAAAEKVRVDLELLPAYMSAPAAMAEDAMEIHPGTPNVYYI
                     QNLEKGDPTGPIFEKADVVVEDDFYVGRQPHLTVEPDVGFAYMDDDGKLIIHSKTIGA
                     HLHLYMIAPGLGVEPDKIALVSNPMGGTFGYKFSPTMEALVGAAALATGRPCFLRYNY
                     FQHMTYTGKRSPFFVNMRFAADKAGKLLAMETDYSVDHGPYSEFGDLLTLRGAQFMGA
                     GYDIPHIRGVGRTVCTNHAWGSAFRAYGSPQSLYSSESLMDELAEKLGMDPWEIRYKN
                     CYRPGATNPNGQAPEVFSYPEMLEALKPRYEAAKDKARRESTDRVKKGVGLSLGCYGC
                     GLDGPDGSEAWAILNQDDTITIATAWEDHGQGADMGAIGTAHEALRPMKVAPERLRFT
                     WPDTSKTPNSGPSGGSRQQVMTGGAIRVACQEMLAGLKKADGTYRTYAEATADGVPTK
                     YVGKFSVPGVACDDKGQGKPFVVYMYGVFMAEVAVNLDTGKTTVEKMTLMADIGKVNN
                     RLVTDGQIYGGIAQGIGLALTEDFEDLKKHSSMTGAGFPFAKDIPDDMEIVYFEGYPR
                     EFGPFGAAGVGELPLSSPHAAIGNAIYQACGVRVHALPALPEKILAGLKGERV"
     gene            29137..31335
                     /locus_tag="PL162_04480"
     CDS             29137..31335
                     /locus_tag="PL162_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQF91309.1"
                     /translation="MEQESLKELERRCVQEELPYCQAACPLKVDGRAFCGAMGLGKFD
                     EARKILSRAMPFPDLVGRLCDAPCERSCLRAERGGAVTLGALERACLDRSRPGPPPMR
                     LPSRGSSVTVLGGGLSSLVCALELAKKGHRVTLWGAEGPLGGHWAREGALPPELWEEA
                     GRLLRHLGVRWEGERGVFPPLPPGGALYGGGDDPGWRGALPEEWDPMTHATGTPGVFA
                     GGAPRAGGPSAVEEAADGKRAAQSIDRFLQGASLLAGREKEFQTETRLFTSLEGVVTE
                     PPREDPVEEARRCLQCECRVCVRECVFLASHGRYPRVYAREIYNNFAVVHGCHQANGM
                     INSCALCGLCETLCPHDFSMADLCRSAREELVARGIMPPSTHAFALQDLAQAQSPRAS
                     GFRPGTRGTRAVFFPGCQLAGALPERTGALTAFLDRRFDGALGVLLGCCGAPAWWAAR
                     RDALDPVVERLGATLLSLGNPPVVLACPSCADMFRRFLPEVPRRSLWEVLLEGELPPG
                     APRREPLAISDPCTTRHDPAWRGALRQVLERLGQEYEELPMGGERTKCCGYGGLQSQG
                     APELARRGAEVRLRESSRDFLTYCAMCRESLSGLGKGVWHVLELLFPEMPPEPATGFS
                     GRQENRILLRERLLGASGIPRESWEDLELGVSPEMRRGLEERRILDSDLRRVLAACRE
                     KGRGLVADDGRALASLRLGEVTFWVEYRLRGEVFEILNAWSHRMTVEVIP"
     gene            31332..31544
                     /locus_tag="PL162_04485"
     CDS             31332..31544
                     /locus_tag="PL162_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HQF91310.1"
                     /translation="MSEASFSFQDEGLWRCACGGTLEMRRVAACYLESRFEIELPVCG
                     KCGFILVPESLARGKMRDVERILEDK"
BASE COUNT         5477 a        10039 c        10742 g         5448 t
ORIGIN      
        1 gcaacacccc gggaaggaag gccttgccca gcttggccag atcttccgcc gtggccatga
       61 gtcctccgct ggcgtaggcc gagacggggc tctcgggggg ggcgttgatg aggcgtcccc
      121 cttcgagggc atatccccgg gcccggtggg gcatgatatc ccgctgcgac cccaggcgaa
      181 gggtggtcat gcccaggggg gcggcgaggt cttcccggac gaaacgctcg taggatcgcc
      241 ccgaaaggcg ctccagaagg atccccagga tgataaatgg ggaattgctg tattgggccc
      301 gctgtccggg ttcgaactcc aggggctctt tggaaaaaag agccagggcc tcctgggggg
      361 tccaatcccg ggcgggatcg gcgctccagg cggggatctt ggtgacctcg gccactccgg
      421 aggtgtgctg caagagctgg cacagggaga tgcgctcccc ctcggggtag ccgggaaagt
      481 aggcggagac agggtcttcc agggtgatgc ggtcttcttc caggtatttg agcaccccca
      541 gggcggtgaa gaccttgctg acggatccca tggcaaagag cgtgtccgcc gcagcaggga
      601 catcttgctc cacgtccgcc gagccgtata cccgggatag ggtttggccg tccggcagca
      661 aaagccccac cgccacggcg ggaatgcccc ccgacgccat ttcctgtctc accaggacat
      721 ccatagggcc gaaatcgaag gaaatccccc gggcctcgcc ggggttccct cccaagagga
      781 ggaatcccag aattcctccg acgaagaaaa gggcacacct catggggctc acgctccttc
      841 cggacttttt taccacctct tccctggccc cggagtcttc tccaagaccc cctcggagga
      901 gcctccgcca gcgcggggag tgtcaaaccg ccgcgaaccc tttcaccctc ctttgcggat
      961 cttcgagctt cccctctcga ccttgggcgg acgggggaca aaatcgccta gggcagggag
     1021 gccgcgaagg ccgccagacg atcgttgagt cgatcgggga cctccagcat atagaagtgg
     1081 ctcaccccgg gccatacctc gtaggtcagg tgaggaaaga gccgtcgcag ctcctcctcc
     1141 aatccctccc cctcggcccg gaccgtttcg gcgccgaaga gggccagcgt ggggacgtag
     1201 gtcggcaggc tctcctcccg ggtggggaga tgcaagaagt tcaccatggc gctatccctc
     1261 acgtaggggg cggtgccgag catcttgtcc agcaccagac gtcgcacggt ctccggggtc
     1321 tccgggacga acatcccctc gatgaaggcc cggacgtgat cccgggcttc gggccccgtg
     1381 gtcagggagg tggcccaccc cttcatcatc tggacgaact cttcccgctt ctggggatct
     1441 gcgggggccc ggacgagggc ggggtccgcc aagaccaacc ccgccacccg gtgggggtag
     1501 cgctgggcga aggcccggca gagggcccct cccatgctgt gtcccaccag caccccttgg
     1561 gaaacccccg cgtcatccat gacggcccgg aggccctcca ctaagacctc cggggtgtag
     1621 gagatcttgg gcttggtgct ccacccgtgc ccggggagat cgagggcgat gacccgccct
     1681 cggcgggcca aaaaggggat ctgatatcgc cagaaggtgt gatcgcaggc ccagccgtgc
     1741 accagcacca gggggcgatc ccccgacccc aggtctcggt acgccacggc gaactccccc
     1801 gctggggcgt agtgcagcgc caaagaatcc tgggccccgg cgggaacggc aaaaaacatg
     1861 gcgaaaagca aaagaacccc taccagagaa cgtctcatca aaacgatcac ctcgcacgaa
     1921 agttttcgcg aaaaaggcct cggagcgaga agggcccctc atcgcgaaga tcggtgaccg
     1981 cactatactc caaaagctcc tccggcggga agagggcata catggcgccc ccggaagggg
     2041 tgcccgattt ttccgccgaa ggcctctgcc ccgccctcgg gatttcccca gggcagggac
     2101 cggggttttt tcggggaaac tggtccggtc cagaggggac cgtccccggg gaaggggtgg
     2161 ccctttatct ccccgggccc cttgcctatg atctccctgg aagcggaaaa aagcttcgcc
     2221 acagaccttt taggaggcga tccccatgga agagcggtcc aacccgaaga atcccaggcg
     2281 agatatgacg tggatgaagc cccggggcgt catcatggac gtgaccaacg cgtcgcaggc
     2341 ccgcatcgcc gaaaaagccg gtgccatggc ggtgatggcc ctggaacgca tcccgtccga
     2401 gatccgcaag gcgggggggg tggtgcgcat ggccgatccc gacaaaatca aagaaattca
     2461 ggacgcggtg tccattcccg tcatggccaa agcccgtatt ggccacttcg tggaggcccg
     2521 cattctggaa gccctggggg tggactgcat cgacgaaagc gaagtgctca cccccgccga
     2581 cgaggagcac cacatcgaca agcgcccctt tgccgtcccc ttcgtctgcg gctcccggaa
     2641 cctgggagaa gcccttcggc gcgtggccga aggcgccgcc atgatccgca ccaaaggcga
     2701 agcgggcacg gggaacgtgg tggaagcggt gcgccacatg cgggccacca accgggagat
     2761 ggcccggctc ctgggggctc cggaagaaga actccccgcc ctggccaaag agctccaggc
     2821 tcccctggag accctttggg agtgccggaa acgggggcgc atgcccgtgg cgcacctcgc
     2881 cgccggaggg gtggccaccc ccgccgatgc ggcactcatg atgcagatgg ggtgcgacgg
     2941 ggtcttcgtg ggcagcggca tcttcgcttc atcggctccc gagcgccggg ccgaggccgt
     3001 ggtggccgcc gtggcgcatt acgacgacta tgcggcgttg gcggaagttt cccggggtct
     3061 gggagaggcc atgcccggca tcgacctccg gacgcttagc cccgaagaaa ccctcgccgt
     3121 ccggggatgg tgaccggtcc ccctcgcccg ccgcagggcg ctttctaatt tctaaagcga
     3181 tggggggcgg acccggagcc cgccccccta ttttttctcc cccgcagcgg ccacgaaggc
     3241 ccgaaagagg gccgccatag gggccgaagt gctttcgaga cgttcggggt ggctctggat
     3301 tcccagaaga aagggtccct ccggagcttc tatgccctcc accaccccat ccggagccca
     3361 ggccaccgcc tcaagccccg gcgccacatc ccgcaccgcc tggtggtgga aggagttgac
     3421 ccgcacgctc agactcccca gcacgtgggc caggcgtgtt ccctcccgca ccgtcatgga
     3481 gtgggaggga aaggtgccgg gatagggttg ccggtgttgg agcaacccct cggggcgttg
     3541 ggaggggaga tcctgataga tcgtgccccc cagggccacg ttgagcacct gacatccccg
     3601 gcaaatgccc aaaaggggca tctttcgccg acgggcggcg cgggccaggg ccatctccag
     3661 ggcatcgcgg tggaggtcca cccctcccag accgggaagg ggctcctcgc cgaagagcgc
     3721 cgggtccaag tcccctccgc cggagagcaa aagcccgtcg aggcccgaaa gccactcccc
     3781 ccaatcttcc ccaaggccat tcccgggggt aaaccctccg ggggtgtgtt ccatctccgg
     3841 aatcggcagt acccggggga tgcccccggc ccgcatcacc gcccggacgt aggcccgggg
     3901 gagggtgttg gtggtgcgtt ggtcgtccag atcacaggtg atgccgatgt gcggtgccat
     3961 ggccaagacc tcctcgtgaa acgctcttcg gggtgcctcc cccgacggga ccattgtctc
     4021 cctgggggag gataaaggca aggagttccc cccgtccggg aggttgtccc gggaaaaagc
     4081 gtgacaactt tgtgactttt tcatgctggt tttgagaaag ggggcccttt tgtgctaaaa
     4141 tggaccctgg gcggatgggc gcctgatggt ggaggaggtg agggcatggc gctggtgcag
     4201 gtggtggagt gggacgagcc agtgacgcgg gaagtgcccg tgctggcgtg gaagtacacg
     4261 aatccggccc agccggagcg gagcgatgag ctgggcacct ggacccagct ggtggtgcgg
     4321 gaatcccagg aggccatcct gtttaaaaac ggccaggcgc ttgatctttt cgctcccggg
     4381 cgccacacct tggggacggc caacgtgccc cttttggcgg ggctcctgag gctccccttc
     4441 ggaggagagt cccccttcaa ggcagaagtg tggttcgtca acaaagtgtc gatgctcaac
     4501 gtcaagtggg gcacccccga tcccatccag atccaggacc cccgatacaa ggtctttttg
     4561 cccgtgcgct ccttcggcca attcggcgtc cgggtggccg acgcccggaa gttcttggtg
     4621 aaactggtgg gcaccgtgag actcttcgac caggagcacc tgaacaccct cttccggggg
     4681 ctcgtcctca ccaaggccaa ggacctgatt tcttcctacc tggtgaagca gggcatcagc
     4741 gtggtggaag tgaacgccca tctggacgac atgtccggac acatccggga gcgcctcgcc
     4801 tccgtcgtgg aagagtacgg catcgaggtg cccaatttct ttgtgaactc catcaatatt
     4861 ccggaggacg accccgccgt ggcggagctc aagaaggccc tggcggaaaa ggcccgcatg
     4921 gacatcgtgg gttacaacta ccagcagatg aggaccttcg acaccctgca ggccgccgcc
     4981 tccaacgagg ggagcagcgg caccctcctg ggggcggggt tggggctggg catgggagtg
     5041 ggaatgggcg gggccttcgg cggcgccgcc gcctccatgg cgggctcctt gagccccacg
     5101 ggcgccaccc cctgcccccg ctgtggggct ttgaaccctc ccggggcccg gttttgcctt
     5161 tcctgcgggg cccttctgga gaactccgtt cccgccctct gctcccggtg cggagggtcc
     5221 ctgccccggg gggccaagtt ctgccccgcg tgcggcgatc gctacgaccc ttgccccacc
     5281 tgcggtgcgg atctgcccga gggggcttcg gcctgcccca agtgcggcgc ccctcgcccc
     5341 ctgccctgtc cccggtgcgg cgcctccagt ccccccggca cgaagttctg cccggggtgc
     5401 ggccaaacct tgacccccgg ccccctcaaa tgcgccgcct gcggcgccga aatgtccccg
     5461 ggggcgaagt tctgccccga gtgcggggcc cgacgggatt gaccccaagg ccagccgaag
     5521 atcgtaatcg aggaggaagg ccatgagaaa gttttccctt agtctgctga tccttctggc
     5581 gctggcggtg ttggcggtgg ggacgggggt gatcctctac ctctccacga cccccgagga
     5641 tcgccatctc atcttctgga tctccctggg caccgtggcc ctgggagcgg ccctttccac
     5701 cctgggggcc ctgatcaacc tctggtcgga cagcgaccga gccctgccct ttccctccac
     5761 cctgtccctg acggccgtca cggggggata caccctcttc gccctggggg cggcggtggc
     5821 ggcgtggcgc tttgtcgact tttccatcac ggggtacatc atcacccacg tggcgggggg
     5881 cacgatcttc ctcgtgggcg gcatcttcgc cctcatgggg ggggtgcaga cctcctcgga
     5941 ggaccggagg gccgcccggg ggcgggcgtg gctggcggat atggcccggg aggtggagct
     6001 tttgacggcg actcgcccgg gagattccgc cgtggcgtgc cgcaagttgg cggaggcgct
     6061 gcgcttcagc gaccccgtga ctccgaagcg cttcgccgaa gaagaccggg cggtggagcg
     6121 cgccgtggcc cgggtgcggg aaagcggcgc ttctccggag agcgtgcagg aagccctggc
     6181 gaccctcaag cgccggaacg atctggtggc ccggggaaag tgacgcggtg ggccgaagac
     6241 caccataatt cactaaaatg taaggagagc gcgtaagatg agcgattttt cgtcggacag
     6301 acagttgaat cctccctttt acctctcttg gcccgtcatc atcatctcct tcatcttctg
     6361 gcccgtgggc ctcgtgctgt tgtttgcccg gctggggagg gaccgccatg cgggcatcac
     6421 caccggcggc gtgctgaagg tcgtgggggt gctcctcttc ctggtttgcc tgctgggggg
     6481 gtacggaatc tacgagaatg agatgaccag cttcggcgat ctcaccgacg acggctatgc
     6541 ctctttggcg gtggcggggg ccttcggcgt gctgggaatc atcctgtggc gctacggtcg
     6601 caccctccag tatcgggggc gcatggtgcg ggagtacctc ggcctcatcg tcaaccggcg
     6661 cttttccaac ctggacgata tcgcccgcat cgtgggaaaa agccggcccc agacggagaa
     6721 ggaaatcgac tttctcatcg aaaagggata cctgcccgag gctctggtgg accgaggggc
     6781 gggccaagtg atcgtcccct cggtgctccg ccacatggag gccgaatccc ggtggaaaag
     6841 ctctcaggaa ggcgagaacc ccgcccctcg ggagaacgcc cccgcagggc cccaggtggt
     6901 ggcctgccgg agctgtgggg cccaggggat gatctttccc ggtcgtcccg ccgtgtgcga
     6961 gtattgcggt tcggcgctga aggtttccgg gggaaggcag tagcccccct cggcagggac
     7021 ccaaactacc cctaggtgag gaggaagaca tgaaaccgaa ggtctttgtg acgcgacaga
     7081 tccccgaggc cgggctagcc atccttcgag accggaccga cctggaagtg tggccccacg
     7141 gcgacatccc cgtcccccgg gaagtgctgg cggaccggat gccccacctc cacggagtgt
     7201 tgaccctgct gagcgacccc atcgaccggg ggatcatcga ccggggcccc caccttaaag
     7261 tcatcagcaa ctatgccgtg ggtaccgata acatcgacgt ggccttcgcc caggcccggg
     7321 ggatcacggt gaccaacacc cccgacgtcc tcaccgacgc cacggcggac ctggcctttg
     7381 ccctgatcct ggggacggcc cgtaaggtgt tggagggcaa ccaagtggcc cgcagcggcc
     7441 gatgggcctg ggcccccgac ctgctggcgg gagaagacgt cttcggggcc accctgggca
     7501 tcgtgggctt cggacgcatc ggtcgcgctg tggcccgccg ggcccaggga ttttccatga
     7561 cgatcctggt ggccgcccga gattccgccg cccgagacgc ggcggagcta ggagcccaaa
     7621 tggtcccctt cgaggaactc ctggaaaaaa gcgactacgt gacgctccac tgccccctga
     7681 ccccggagac ggcggggctc atgaacgaag aagccttccg gcgcatgaaa aaaaccgcca
     7741 tcttcatcaa cacggggcgg ggtaaattgg tggatcagcg agccctgggg caggccctct
     7801 ccgaagggtg gatcgccggg gcgggcctgg acgtcttcga ccccgagccc ctgcccgcag
     7861 accaccccct tttcgccgcg ccgaacctct tgcccttgcc ccacctggga tcggccaccc
     7921 ggcgcacccg ggagcgcatg gcgacgatgg cggcggaaga cctagtggcg gtgctggatg
     7981 gcaagatccc ctgccatccc gtgcggatct gaaggccccc atgggatcgt ccctcggtga
     8041 caccgtaagg gccatcgccc tggaggccct ggcgacctgc tcccccgagg acgccgtgag
     8101 gcgcgtcctt cagcccatcc cctcgggggc ccgggtgggg gatcgacagt gggatggccc
     8161 cttctacgtc ctctcggtgg ggaaggcggc gagcgccatg gcccgggcgg cgtcggacct
     8221 cctggggaac ctggtctggg cggggagtgt cctgacccgg gacggttaca gcgacgagat
     8281 ccccttcatg aactgcctcg aggccggaca ccccctgccg gacgcccgga gcctcaacgc
     8341 cacggcggac atcctgcggg aaatcgacgc cctgcccgat gacgtgacga tcctgatgct
     8401 cctttcgggg ggaggatcgg cccttttcga agcccccgac aagggcattt ccctggaaga
     8461 catccgcgcg gtcaacgccg agctcctcac ctgcggggcc tccatcgccg aagtaaacgc
     8521 ggtgcgcaag cacctctccc gggtgaaagg gggcaagctc ctgcgccgca tatccccccg
     8581 atcttccttg gccctggtgg tctccgacgt ggtgggggac gacctggcca ccatctcctc
     8641 ggggcccacg gtggccgacc acaccacctt cgccgatgcg gtggaagtgc tcaagcgccg
     8701 agatctttgg ggaaagaccc cgaaaaacgt gcgccacgtc ctagaagaag ggctcaaggg
     8761 cctccgagaa gagaccttga agagcgtgga gggctatgac acctgggccc aggtgatcct
     8821 ggacaaccga tccctctgcc gtgcggcggg agaggccgcc aagatccggg gctttacccc
     8881 cctcttcctg ggatccttct tctcgggaga ggcccgggag atcggcacct ttgtcggaga
     8941 gatcgcccgg gagatccggg cctcgaagca ccccgggccg ccccccgtgg cggtgatttc
     9001 cgggggggaa tcggtggtga aggtgcgggg caagggcagg ggaggcccgg ggcaggaagc
     9061 cgccctggcc tgcgcccggg cgctgcgagg cctttccggg gtggcctttc tggccatgga
     9121 tagtgacggc accgacggcc ccaccgatgc ggcgggggga gtggtggacg gcaccacctg
     9181 ggatcacatg gaatcccggg gcctcaatcc cctggacgcc ctggaacgca acgatgccta
     9241 cgccgtcctc ggctccggag ggggcctttg gaagacgggt cccacgggaa acaacctgaa
     9301 cgacctgaga atcctcctgg tggaggaatg aattggggcg ggaatgacgg agggccccct
     9361 cgggacccga agaagggagg gaagggaacg tgggcagaaa agttcttcga gtgattcacc
     9421 gtgaccgctg catcgggtgc ttctcctgca tgtatgcctg cagccggacc tttgcgggga
     9481 tggccaccac caatcgggcg gccctgaggg ttcgggccta caccggtgtc gagggagcct
     9541 tttccctccg cacctgcctg cgatgccccg aaccggactg tgccgccgcc tgcccttctg
     9601 gggcgcttaa aaaagtcgaa ggggggggag tgcgcctgga caaagagcgg tgcctttcct
     9661 gcggagcctg cgtccaggcc tgcgtgatcc gagccctgca gtggaacgcc gacgaccggc
     9721 gccccatccc ctgcgtgcac tgcggcacct gtgcccgata ctgccccaac ggcgtcctgg
     9781 ccctggaaga agaagcctaa aaggagggcc ccccatgcag gaaaccatcc ttcacataga
     9841 cctgacgacg gggacggcgc gccgagagcg ccgccccgaa ctctttgatc ggttcctggg
     9901 aggtacgacc ctgggcaccg aactcctgtg gcagtacgcc cccccggaag tagaccccct
     9961 gggggagcag agcgccctga tcttcgtcac gggtcccttt tcctccgttt ttcccgtggc
    10021 caccaagaca gtggctctct tcaagtcccc cctcaacggc gaactgggag agacccacgc
    10081 cggggggcgc ctcgccatgg cccttcgaga tgcggccatc gacgccctgg tgatcaccgg
    10141 aaaaagcccc tccccgacct ttctttccat cgagaatcac gaagtgacct tctcctcggc
    10201 ccgcacggtg tggggtcagt cagccctggc gtcggagcgc atccttcggg aatacgccgc
    10261 gccggacggg cgaaagggct ccgtggttcg catcggccct gcgggggagc gcctcgctcc
    10321 cctggcctgc gccacggtgg acggagggcg ccacttcggc cgtctgggcc tgggggccgt
    10381 catggggtcg aaaaacctca aagccttcct ggtctccggc accgggggcc ccccgatcat
    10441 ccaccgggga gaacatcgcc aggtctacga cgccatctac gaccgggtgg tggcctcaaa
    10501 tgacatgaaa aaatatcacg acctcggcac ggcggggaac atcttgcccc tcaacgccat
    10561 cggagggctc cccacccgga atttttccca gggctttttc gagggggccc aggccctttc
    10621 gggggaggcc ttcgccgagc actacctggt gcaacacatg gcctgcgccc actgcccctg
    10681 cggctgcatc cacatggcgg ccctccggga gccctttgcc ccatttcact accgcaccac
    10741 caagattccc tacgattacg agctcatcta cgccctgggc accctcctga gcctctcttc
    10801 ccccccggag gtcctatccc tggtggacgt ggtggaaaaa gaaggatggg acgccatggg
    10861 gatgggcacc gccctggcct gggcctcgga ggccttcgtt tccggggtgg tggggctccg
    10921 ggagaccgaa ggccttcccc tgcgcttcgg cgacggagga gggtacctac aggcgatgca
    10981 tcgcattcga aaaggcgagg gggagttctt ccgcgacctg gagcgaggag tggccttctg
    11041 tgcggcaaag tacggaggag aggccttcgc catacacttc ggcggcgtgg gggccccggg
    11101 gtacctcacg ggggaaaatg ccctcctcac ctatctcatg ggggtgcgcc actcccacct
    11161 ggacgacccg gggtacggca cggatcaaaa gctcctgggc aaatccctct ccctggaaga
    11221 gcaggtgggc gcggcggtga aagacgccca gtggcgcatg gtgaccaatt ccctggtgac
    11281 gtgcctcttt gctcgggggg tctacgacga ggagatggtg ctggcgggcc tcggggccct
    11341 gggcctttcc tgggacggag aaaagctaaa gcggatggga gaagaggccc tgcggcgaaa
    11401 atacgcctac aagatcgcct gcggcgccga cccccgccgg ggaaagatcc cggaaaagat
    11461 cttttccggg accaccgcca cgggccccgt ggaccggggt cgcatggagc ttcgaaggga
    11521 gctctacgcc cggatggtgg gcctccccga agaggtgtga aaaagatcgc gagggcccga
    11581 atgggccctc gcgatcttag tttccctccg catctacgaa acggagcgtc tttcccatgc
    11641 gctccaccac gggatcccac tcctccttgt cctcttcggg gtagacgaag tggaccgcga
    11701 tgatggtgtc cccgtcggca gagaagcgca cccgttggta gaagatggcc ccctcggcgg
    11761 tgaaccccga caggacgcac cagttcttcc ccgtggcctc gtaggtcacc cgggcccttc
    11821 ccccggcgtt ggcaaaaccc tctttgagcc actcccggtg atcttcgggg gagtgcccca
    11881 gggcgttgaa ggagccgtag accgccacgg acccttggcg ccccgggggg gtaaacgtcc
    11941 ggccgtcgtc gttttccggg gcctcgcccg ccgggaaatc cgcaggcacg tccaccgtat
    12001 agccgaagcg tgggttggta taaaccgccc aggaggaaga aaatcccgga gcggaacttg
    12061 cccccagggc ccaccacgcc accgccagaa ccaacgcctt gaccgccgcc ttacccccca
    12121 tggtctcccc ccctattctc tagcccgaga gtatacatct ctttccccgc cccgtctcgc
    12181 ccctcggtcg accttttagg cgatgtcttt acccgacttt cgctttcttt tgccgggagg
    12241 cgcgcatctt ccagccggct ttaccgcgaa atgagagcta cgatggtgac ctttctcccg
    12301 aaggaggaca tctcgtagtg atgacttttt atagaaagct agtgatggca aggatttatg
    12361 gcttctttgt gtgaaagttg ggtcctagtt ttttgaaaaa tctagcgatt tttcccccga
    12421 gacgacgaac cggccttgac acctccgaca tcttgggaca gaattgccgt gaagcaaagt
    12481 ttatttctag ggagggaatg tcatgcgaag atcctttgtg cgatggatgg cggcgatggt
    12541 ggtcctcggt gtcatggtct ccggggcctc ggccacggtg tttatcaaca tcggcaccgg
    12601 aagcaccggg ggcacctatt accccgtggg ggccgccatg gcgaagatct ggaacgaaaa
    12661 gatcgaaggc atgaaggcca acgcccagtc caccgggggc accgtgaaca acctccagct
    12721 catgacggac aacgaggccg aagtgggctt catggacggg gcctactacg ccgcctacac
    12781 gggcacagga aagtttgaag gcaaacccat ggaaaacctc cgggccctgg tgcccctcta
    12841 tcccgaaccc gtccagctca tggtggcccg ccagagcggc atcaagaccc tgggagagtt
    12901 caagggcaag aaagtctcca tcggcgccgt ggccagcggc accgaggtga cggcccggca
    12961 gctcctgaag gttgcgggca tggaccccga caaggacatc accccggaaa acctcggcgt
    13021 gggtgacacc acgggggcct tcggcgatca gcgcatcgac gccgccgtca tggtgggggc
    13081 tttgggcatg gcgggagtgg tggaagccac caccctgggc ctggtggact tcatcgacgt
    13141 tccccaggac attctggaaa aagtcatgaa ggaaaccccc tactgggtgc ccttcaccat
    13201 tccggcaaat tactacaagg ggcagcccaa cgaggtcaag acctatgcca gctggaacat
    13261 cctgggggtc aaggccgatc tcgatgaaga actggcctac cgcatgacca aggccctctt
    13321 cgagaaccgg gccgatctgg tgttggtgaa ctccaagatg gaaaacatgg ctccagagaa
    13381 cgtaaagttc atcatgatcc cccttcaccc cggggcagaa aagtactaca aagaaattgg
    13441 tgcggtgaag tagcgagacc ttccaaggcg aaaaaaagcg gggcggccgg agagccgccc
    13501 ctttcgcatc ggagaaaggg ggactcctcg tggcagaaga aagtagagag aacctcattg
    13561 agaagaactc cttgggagaa aaagaaattc agcacctggt ggagcaatac gatgtggaaa
    13621 gccgtttccg tcgcctttcc ggttttgcgg gaaaatttgc ggtatgttgg tgtgcggcca
    13681 tgtcctgttt tcacctctac acggcgggga tcggccttct gcccacctcc atccagcggg
    13741 cggtgcacct cctctttgcc atggtggccg tgtttctgct ctatccctcc caccaaaggg
    13801 tcgataaaac ccggattccc tggtatgact ggatcttagc ccttctggcg gccatcggta
    13861 cggggtacat cgtctttttc tttaacgaca tcgcctaccg gggggccaag ccccttcccg
    13921 tggacatcgt gctggggtac atgacccttg ccctggtggt ggaagcgggg cgccgcatcc
    13981 tgggaaacgt tctccccatg ctggcggtgt ttttcctgct ctactgcttc ttcggccggt
    14041 atgcccccgc catgttcatg caccgggggt attccgtggc ccgggtgatc caacacatgt
    14101 atctcacgtc cgagggcatc ttcggcgtgg cactgggggt ttccgccacc tttgtcttca
    14161 tgttcgtgct cttcggcgcc ttcctcgcca aaagcggcgg cgcccggctc ttcaacgagc
    14221 tggccctcgc cctggcgggg cacagccccg ggggccccgc caaggtggcg gtggtggcct
    14281 cggggttgct cggcaccatc aacggctcct cggtggccaa cgtggccacc acaggggcct
    14341 tcaccatccc cctcatgaaa cgggtgggat accctccgga gtacgccgga gcggtggaag
    14401 cctgtgcgtc caccggcggc cagctcatgc ctcccatcat gggggccggg gcgttcatca
    14461 tgagcgagct cctgggggtg ccctatctct ccatcgcagc ggcggcggct ctgccggcat
    14521 tgctctacta cagcgctatt ttcatcaacg tccatctgtg ggcccgggct ctgggattgg
    14581 tgggcctcga ccgggcctcc ctgccccgcc cccgggatgt ccttcggcga gacggccatc
    14641 tcctcgtccc tctgggggcg atcattgcca tgttgctgat gaaattcacc ccccttgcct
    14701 cggccttctg gggcattgtg gcggtggtgt tggtgggggc tctccgctcc agctcccgcc
    14761 tgggatggcg agagatcctc gccgccctgg acgagggcgc ccggggctcc ctgggggtgg
    14821 ctatggcctg cgccctggtg ggattcgtgg tgggcgcctc gtccctcacc tccctggggc
    14881 tgaacctttc caacaacatc atccaccttt cgggaggaaa cctgttcttc atgcttgtgc
    14941 tctccatgat tgcctgcctg gtgctgggca tgggccttcc caccacagcg aactacatcg
    15001 tctgcagcac catcatcgcc cccgcgctgg tgaagctggg ggccctgccg ctggcggccc
    15061 acctcttcgt cttctacttc ggcatcatgg cggacctcac gcctccggtg tgtctggcgg
    15121 ccttcaccgg cgcgggcatc gccggtgccg atcccacccg gacggggatc caggccgcca
    15181 ccatggcgct ggtggcgtac ctcctgccct acagctttgt ctacaccccc atgatcttgc
    15241 tgcaggacgt ggcccctctt cctctgggga ttctcgtggt ggcttccgtc ctgggggtgc
    15301 tgggactggc gtcggcgggg cgggggtgga tgttccgcac gctgggagga gtggagcgca
    15361 tcgtggcctt ggcgctgggc gtgggggcct tttggcccca tccggtggtg aaaatcgccg
    15421 cagcgctggg actggtgagc ttcctgttcc tggagcgcct ggccgcccgc cgggccgcag
    15481 agcggagcgt gcagggggcg taaagaactc aacttttccc aggggcgctg gggcgagagc
    15541 ccgtgtatgg ggggcgcgag cgccctgtag gggcgaaaaa cctttcgccc cggtcctcgg
    15601 tcggtgtggg gcagatgtcc ggtgggcgat gcctctttgc ccggaggcgt gcgcatttcc
    15661 ccgggggggt cctgtaggga cgaaaaacct ttcgccccgg tcctcggtcg gtgtggggcg
    15721 gatcctcggc acgaggccga ggagatccgc agggggcagg acgtggaaac gtcagggacg
    15781 atgggagagg ggaagctcca accccgccgc gtccagaagg ggggcgtcgt acaggagatc
    15841 cgctgtggcc ccctgggcga agaggtgccc ttcccggagg atcatcaccc gggggcatac
    15901 gtccagcacc aggtccaggt cgtgggaagc cacgatccgg gcttcgggga gggagcgcaa
    15961 cagctcgatg agatggcgcc gggcccgggg atccagggcg gcgctgggct cgtccaaaag
    16021 gagcacgtcg ggggcggggg ccagggcggc ggcgagggcc gcgaggcgct tttctcctcc
    16081 cgagagggcg tggggcatgc ggggagccag atgacgagcc cccacccgtt ccagagctcc
    16141 ccaggcggcg gcatgcacct cctggggaga gagcccccgg tgccgggggc cgaaggccac
    16201 gtcctcgtac accgtgggaa gaaagagctg gtcgtcggga tcctggaaca ccacccccag
    16261 gcgacggcgg acggagggag gaaagggggt ggggatcttc gccccatcca agacgaaacg
    16321 tccccgggag gggggaagaa ctcccgccag aaggagcaac aacgtggatt tccccgcccc
    16381 attggccccc acgatgccca aggcctcccc cggagcgagg tccagggaga ccccctccag
    16441 cgccccgggg gcgtcggggt aggaaaaggc caggtcctgt ccctgaaaaa agggggcggt
    16501 cacgaaagtc ccccgatctc cgccaggcgg gagaggggcc tcaggcgcac cagaagaaaa
    16561 aagacccccc agcccgccag aaagagcaga ttccggcgat ccagaggcgc ttcggcgcct
    16621 tccagagccc ccatgccgaa ctcccgggcc gccatggccc gggagatgcg ttctccccgg
    16681 gccagcgtgc gtagcagcaa gctccccaga aggggccccg ctagggcgag ggagggggat
    16741 tgtccgccgg tgcgggcccc gtgggcccgg agcgtgcgga gggcctcgtc cccgaggaga
    16801 aaaagatagc gcaccaggag caggatctgg agggccagca cccggggaac cttcagccgg
    16861 acgaggccgg agcagaggtc gtccaggggg gtggtgcgaa gcaacagcag cgccccgctc
    16921 accgaaagaa caaatttcat cacgatgccc agcagggaaa accaaccggc ggcgagggcg
    16981 atgccggggg cgatgataac cggcgtgcgg tgccacaggg gctccgccac ccccaccaaa
    17041 agaaccaggg gggagaggac cagggcccgg cccagaagcg ccctgccggg aattcccgcc
    17101 agggcgccga cgacgcaggg aaaaaagagc aggggggcca ggtcccccgg agcagaggag
    17161 ggaaaggacg ccgtcaccac gagaaaaagg gcggtgacgg cgatttttgt ccggggatcg
    17221 aggtcgtgga tcggcgaaag cctccgccca aaatccaggg tgtgggaggc ggcgcggagc
    17281 cggtgttcga gggaggtcac gaagaggcgc ggcacgcctg ccgccgtcgc agcccccatc
    17341 ccagggcgag ggccaagagt cccgtcaaaa ggccccccgc gatccccgcc agagatgtgc
    17401 cgccactcac cgcaggccag gaagcatcac cttccggagc ggaactctcg ggggaaatgt
    17461 ccgaagcggg gagggcgtaa tcgggcaaga gggcggtacg ttcctggacg tttgccaggg
    17521 cggtgtgcac ggaagtggcc gggccctcca cctcttcctg gcccgtggcg tggaacaggg
    17581 accattccag gccgtcgggg tgggtggagg cgaaccagga aagcactccc cccgtgagca
    17641 gggcaagaac cacgagggcc atgaccagcg gttttccggg cgagacggaa cgttccgggg
    17701 cgatgccctg aacccgacgg gcgaagagca ccaccgaggc ggtgatgaac ccttctccga
    17761 cgccgatggc caggtggatg gggagcatga gcaacacgaa ggtgccgaag gggagcgagg
    17821 aaattcccga ggcgccggtc tcaagcacca ccgccagagc tcccagcgcg aggcccacga
    17881 tggaggcggc gagggaggcg ccgaggatcc gccgcgaaga ggcgttttct cccgtcatag
    17941 gccggaaaat caggggatag gccacgaagc aggaaaagaa tcccatgttg aagatgttgc
    18001 accccagggc cagcagtccc ccatcggcga aaaagagcgc ctggaccgcc agcaccgagg
    18061 tgagcaccag aaacgccgca tggggaccca gcagagccgc cagcaggagc cctcctccca
    18121 gatggccgct ggagcccgtg ccgggaatgg tgaagttgat catctgggcg gcgaagacaa
    18181 aggctcccag ggctcccatg aggggcgccc gggagggggc cggatcctgg tcgagtttcc
    18241 gggcagcata tcccaccaaa cccgccgaga cggcccacaa cgccccgccc acggcggggg
    18301 acaacagcgc gtcagccatg tgcatgtcca tcgctccttc gaaagtggtg ttacgaatag
    18361 cattttttgt agcactcgcg tggagggatg tcaagggggg gagtcctttg cggtggtggc
    18421 tcgggatgcg gggcctcccc gagaaggacc tggaggcctg ggctttggac cgctcctctg
    18481 gaggccctcc ggggtttgcc tcgaagcgct ccccggggac gaaggcccgg aaagtcgtgc
    18541 ggtggtgccg caacgaaggg cccccggaag cggataaggc ttccgggggc cccaagggca
    18601 aggctctcgg gaggtgccgg atggccgacg tcaggcccgg ggtagggcgc gccgttcctc
    18661 ggattgatcg atgcggaact ggctcaggat ctgttccagg tgggccacgt tttccgaaag
    18721 gtgtgccgcg tttcgagcca cctcttggaa ggaatccgcc gtctcttccg tggaggcccg
    18781 gatggtgccc aggctttcca cgatggcgga ggccccgtgg gtgatgctgt cgatggcggt
    18841 ggccatttcg tggctggagg ccgattgctc ctcggccacg gcggcgatgt tttgcatgac
    18901 gtcgttgacg ccgacgactt cccgaaggct ttcctgaagc tgtccttgga cgccctgggc
    18961 ggcctttacc acttcgttta gggtgccctc ggaggcttcg gtggccttga tggaatccgc
    19021 cgcagacttt tgcagcgtgg tgataatgcc cccgatctcc ttcgcggcgt ggtttgattc
    19081 ctcggcgagc tttcgtacct cctccgccac cacggcaaag ccccttcccg cctcaccggc
    19141 gcgggcggcc tcgatggcgg cgttcagcgc caggaggttc gtctggtccg cgatggaggt
    19201 gatggccccc acgaaattgg tgatgtccgt caccgatccg ctcagggtct ggatggtatg
    19261 gcggttggcg tgggcctgtt ctcccacggt gacgatggac tgaatgaccc cggccatgcg
    19321 tgagaccact tcttccgtca agtgagtgga tttggtggag gaatgggctc cggcctcggc
    19381 ggacttggca gaggcgctgg cgctggaagc cacctcttcg atgcccgcat tggtttcctg
    19441 cacggcggcg gcgttggact cggaaaggac tacaatctga tccacggcgt cccggctgcg
    19501 atccgccacc acggtggacc tttccgagag ctctgccaga gatcgggcct gtgcccccac
    19561 ttctccggcg acttccacca gctccctcac catggcggcc agcttatcca cgaagaggtt
    19621 gagggattct cccaggagcc ccacctcgtc gtggctcgcc acgtcgaggc gcaccgtaag
    19681 gtccccttcg ccctggccca ggtcctgcac gacccccacc atgcgccgca gtggcttggc
    19741 gatgcgaaag agcaccagaa ggctcaccac cagcagcacc accgcgatgg ccgccgccgt
    19801 aatccccagg cggcttacca gggccgattg ggctccttgg gcctccttct cgaaggtgga
    19861 gatgagatcg gtcttgacct gttcgttttt cttaagagcc gcttcgaggt ggtccgtggc
    19921 gaggcccacc tccacctggc cgatgctttt gccgtcgtag accacaccgt gggtaaagac
    19981 ctggaccccg gcgccgccct ctttcttctc cccggccacg gccttattgc catcgtcgta
    20041 caccacggcg taggccaggt tggggagctg gagcatcccc tgggcgtact ctttcagcaa
    20101 ctcctcctgc agattccaga ggggcgaggc ggagatgaag gccaaatact gcgccatgga
    20161 ggcgccgtaa gtggcggcgg actcttgatt cgcctggttt ttgttctgga tgtcctcggt
    20221 gagcatgcga tctgccacgc cgctggtcgc ccgaagcatg tcctggctgc ccgaccgggc
    20281 caggaaaagg aggaccgccg ccagcacgag gatacccagg acgatgagag cggcaagctt
    20341 tgatacgagg ctatttttca taatattctc ctttcatggc cttcgatcgc ttcgggggat
    20401 cggaaaagcc tagggtggtg gggagctatt tccccagcaa cttatccgtg gtgccgtcgg
    20461 ccttcatggc tgccaggacc tcgttgagct tcgggaccag ggggagggcc ttgcccttgt
    20521 tggagaaggc caggtagaag ggcatctccg ccagcacggg cttttccgcc acatcgatct
    20581 tgcccctcag gtcatcgtgg gcgttcaccc agtatttgcc ggagatctcg tagcacacga
    20641 agaggtccat gcgccccgcc gcaagcttct cgaagctctt ggtctccgac tctccctcgt
    20701 ctagtttgat cccttgctct tttactcccg cattgaactc ctcgccgtag ttgtaccccg
    20761 ccgtggtgcc aatggcgaga tcttttaggt cgccgtagtt ttcccaagtg aatccgttgg
    20821 gatatttctc cttgacgtag aaaaaaacca cccggtccac caccacgggg tcggagtagg
    20881 tcatgaactt ctcgcgctcg gccgtcttgg tgacccccag caggatgtcg ctatcgcctt
    20941 cctggatgct ttttaggcag cgcttccagg ggtagagcat gatgtttgct tcgaccccca
    21001 ggcgggaaaa ggcctcctgg cacacgtcga cgaaataccc tcccgtgggg ggcttgttgg
    21061 tctcccccac ggaatagggg ggccaggggt cgaggatgac ggaaacgctc ttaatatcgt
    21121 cggcgagggc gacggtgccg aaggaaatca ggacaagggc gacggtcaag ggcagaaaaa
    21181 acctggtctt cataaaacac cctcctcatg tgagttattt gggcgaaaac gaccgctgac
    21241 gcaccatggg cctccgtaac ggggcggcga tcccctcctt cctgggagta cgctaaaagg
    21301 attccgggaa aaagagccac gctccaaagg ggcgaccaat gtcgccagcg acactatagc
    21361 agacgagagt ttttttttaa agggcgagat gggcggcccc ggtcgtttga cgaccccctt
    21421 tctccgtcgt gataaggatt tccccttttt gtgcgataag aggtggagag aataggatgc
    21481 ttcccatcgt tcctccgggc cctgaaaacg tatccccggg gttgttccgg aggggatcat
    21541 gagatgcgcg cccttcgctc cttcgggggg ggcgggcgcg aaaagggcgg cgtcgatgcg
    21601 aaggtctccg gaggccccgg tagcggcctt cccgttccca tccaattcgg aggctttctg
    21661 atcaccaccg ggaggccgag gccctcatat ggtatcctgc aaaaagggtg ggggcgtccc
    21721 ttgtcccttt tcccacggcc catatccctt cgagaagcga ggtttgggga agatgacagg
    21781 gtttttccgg cgatttctga agctcatttt tggccttttt ctctatgccg tgggcatcgt
    21841 gctcaccatc aatgcccacg tgggatatgg cccctgggat gtcttccatg tggggctggg
    21901 aagggcccta gggatcacct tgggcaccgc cagcatcatc acgggcttcg tcatcgttgc
    21961 ggccacctat ctcatgaagg aaaccctggg gatcggcacg atcctcaaca tgtttctgat
    22021 cgggatcttc atcgatgtga tcatgcacgg ggagcttatc ccggtcatgg accggacgat
    22081 tccgggcgtc atcatgctcc tggcagggat gtacgtcatt gccctggcga cgtactttta
    22141 tatcggctct ggtttcggca cgggaccccg cgatgggctc atggtggccc tgacgcgcag
    22201 gtcccggctc cccgtggggc tcgttcgagg gctgatcgag gtatcggcca tcgcctttgg
    22261 gtggtttctc gggggcatgg tgggcatcgg cacggtgatc tacggcctgt tgatcggctt
    22321 ttgcatccag agcacctttc gtctcctcaa gtttgacccc acgaagatct cccatgcgag
    22381 catcctggaa agctgccggg agctgcgggt cgccctcggg gaccggggag cctagggccg
    22441 aacctccggg atcgggggtg gggcgttccg ggcccctcga agaaaatttt tttcaggagt
    22501 gaagaacatg atcgacgtgg tgctttcccc cggagagacc cttccccggg tttcctcgtg
    22561 gcttgtggtg gacctcctcc gggccaccac ggccatgacg gcgtttttcg aggcgggagg
    22621 agaagagctt ttgcccgtgg ccacggtgga agaggcccgg agcctcaggg aaggtctcgg
    22681 gaccgcatgg ctcctcctgg gggaacaggg gggggagcgc cccgagggct ttgactacgg
    22741 caattctccc cgagaaatcc gcgccagtcc tctccagggg cgccggggta tcgtggccac
    22801 caccaacggc actggggccc tcctgcgggc cgcggaagcc ggaggcgccg tgggggcggc
    22861 ctgtgcccgc aacgccgatg cggcggccca ctttgccgct cggcgtgggg ggggtgtggc
    22921 ggtgctctgc gcggggctcc acggccgcct ggggctcgac gacgtgttct gtgcgggcct
    22981 gttggtggaa cgactccggg acctcacggg acaagacctc ggcgacggag cccggttggc
    23041 cctcatggcc tgggagaagg tgcgcccgga cctcctcggg gcggtgcgcc gtacggtgca
    23101 cgccgggcgc ttggcgttga agggcttcga cgaggacgtg gcggatgggt gccagatggc
    23161 cgtctcttcg gtgatgcccg tgttggtgcg gcacggaggg cgcctcgtgc tggtgcggga
    23221 agagaaggac cgtgccgaag tcgctgagga cctagggatc gggactccgg atcgccgcag
    23281 aaaaggatgc cgccctggcg gaacaccctc ggagggtcgg aaatgtctcg ggaggtgaaa
    23341 tcatgaaccg tggaggattt tcctggaagc gctttttagg catctcggcg gccaagagcc
    23401 ggatcagtcg caaaatcggc atccccctga cccgatcggg gcgccagcgg aaggtggggg
    23461 cggccctggg gtgcgtgtgg cccctggcgg gggtgatgtt gctggctgtg gcggcgacgg
    23521 tgagtttttt cctttagggc catctctcta cgctccctct ccccgggagg ccaggacgat
    23581 ccccgcgagg atgcccccca tccccgccgc cttgacgagg gtgagccctt cgccaaagag
    23641 gatccaggcc aagagggtgc tacccagagg ttcccccagg agcgccacgg agatgaaccc
    23701 cgccgagagc caccggaggg cccagttgta gctactgtgc cccagcagtt gggggatgag
    23761 ggccatggcc ccgaaggccc cccaggtgcc ggaagaatac cccgccacgg gaagccccgt
    23821 cagaagcact ccggcccaga gaaaggccgc cgccgtgccg tagcacagga aaatgtagga
    23881 cagaagcgag agcttggggc gcacccggcg ccccaggagt aggtaggccg cagcggccag
    23941 ggcccccgcc agggccagga gatctcccca gagggcctgc ccccccaggg caaaatcgcc
    24001 ccctcccacc acggcgcccc cggcaaaggc cagcgccatg cctcctgcgg tccaccggga
    24061 aaagcgttcc cccgccacca ggggccccaa aagccccacc caaaggggaa tggtgttcac
    24121 cagcaccacg ctgctcgcca ccgaagtgta gttcaacgag gcgatccacg tggcgaaatg
    24181 gagcgcgaga aagaaccccg cccctcccac ggtgacgagg tcacgccgag ggagattccg
    24241 gagctcgccc caccgccttg ccagggcaaa gggggccagc accagggtgg cgagccccac
    24301 ccggtaggcg gcgatgacca gcgaaggggc gtcggccatg cgggcgaaga tggcccccgt
    24361 ggagatggcg ccgatgccga ggatcagggg aatcaggggg gagatggggg ggcggtcggg
    24421 ggtcatggga caccctcctc attgtatggt atgtataccg aaatagtgtg cccctcaaaa
    24481 ggccttctgt caaggggggc ctcgcggtcg gcccgggttt cggcgggacg ggggggatcg
    24541 gcccagggga gctaaggatc tcccggggtc tgggaagggc gtcggtgaag atcagagctc
    24601 ggtcacgccg tagggggcga gggcggacca ggggagcacg aaccgggcgg tcttgagggg
    24661 gtccaccacc tggcagattt gcaaacttcc cgcgatgctc aaaagatagg ccagttcctg
    24721 ccaggggagc cccaggcggg gaaagagcac ctcggcgagt tctcgcacgg cctggtttgc
    24781 cgcgtcgtcc aaggtggggg ccgaggcgat ggcggcgagg ccttcggggg tggccaccat
    24841 gggacccgcc agggagaggt cctttcgcac cgaaagccgt agcgtcaccc gccccgcgat
    24901 ctccaccccc gccccgctga tctccccgtc tcccatggcg gcatggaggt ctcccagggc
    24961 gaagagggcc cccggcactg ccacgggaaa atacaccgtg gctcccgggg cgatgagggt
    25021 ggtgtccatg ttgcccccat ggctccccgg agtgccacag ttcaccggat ctcctccggg
    25081 ggctacgccg atgacgccga tcatggggcg cacggggagg ttcagccggt cgtcgaagat
    25141 cactcgcccc cggcgcacgg ggacgaggcg ggagtggagg gcgtcgaact tcgatgccag
    25201 cacgccgaag tcgtgtcccg ccaccagcac cccctggtcg tccatggtga tggcctcgat
    25261 ggtggcggcc aggacgtcgc cgggttccgc cccctcgacg aagatgggac ccgtggcggg
    25321 gtttatccgg tcccaatcca gggatgccag ggtgtcctcc gggcgccgga tctggttctc
    25381 gaagcagtcg caggtctcga tctccagacg gctgccgtcc ggtacccgga gtcggggagg
    25441 atggtgtcgg gagaacgaat acaccaaacc gctggagtcc acggagtacg tcacggcgct
    25501 catgggtcca cctcctcaag tgttacctcc ccgaggggga gcgtcggagg caacatagca
    25561 caaggacctt tcgttcgtca atttatttcc ccgataagtt ccgatatgac aaaaatcatc
    25621 gggagtttga aatgctttcg gttcaccgcc cccccttcgc ccccccttcg tgaagtttgg
    25681 cggggggggg cggcgttggg gccgtgaggg gcaattgaca gggggtgctt ttttatgata
    25741 ctgtgatcaa aagttttgcc gctctgtggc gatgccaggg ctttcttgaa gggcctttgg
    25801 gccccgggca tcgtctcggg cgggacgaaa cgtcatagtg ggaccttccg agcgtcacgg
    25861 cctaccaggg aattcccctc acggcgtgcc gccgcttagg gtatgcccgg aaacgggcat
    25921 gcctcccgcg tttggcaagg aggggaagat cgtccgtacc tatcgggggc ggccctcttt
    25981 gtgggggctg cccccgtggc gttgtggcgg agatgggggc gagggccggg gcgatccgct
    26041 tccggcagga gagggagagg ggtgaggacg ggtgattcag aagagatttt tcgtgaacgg
    26101 cgtggagaga aacctgattg tggatgggga atcgtccctg gcggacgtcc tccgggggca
    26161 gctggggctc acggggacca aggtggggtg tggcacgggg gagtgcgggg cctgcaacgt
    26221 catcctgaac ggcaagctgg tgcggtcgtg cgtttacaag atgaagcgag tggaggacgg
    26281 agaccagatc accaccatcg aagggatcgg cacccccgac gagctccatc ccctgcagcg
    26341 ggcctgggtt tcttccggag ctacccagtg cggcttctgc tctcccggtt tcatcgtctc
    26401 ttccaaggtc ctgctggacc ataacccgaa ccccacccgg gaagaggtgc gggagtggtt
    26461 ccaccagcac cggaatgcct gccggtgcac gggatacatt ccccaggtgg acgcggtcat
    26521 ggccgccgcc caggtgctcc ggggcgaggc tccggaggaa tccctcgact tcaaaatgcc
    26581 cccggacggg cggatctggg ggagcgccta tccccgcccc acagcggtgg gcaaggccac
    26641 ggggacgctg gactacgggg cggacctggg gctcaagctc ccgacgaatg ccctccagct
    26701 cgccctggtg caggccacgg tgagccacgc gaacatcaag ggcgtggatg tctccgaggc
    26761 ggagaagatg cccggggtgt acaaggtggt caccgcccgg gatgtgaagg ggaaaaaccg
    26821 catcaccggc ctcatcacct tccccacgaa caagggggac ggctgggatc ggcccatcct
    26881 ctgcgacgag aaggtcttcc agttcggcga cgccatcgcc atcgtctgtg cggacaccta
    26941 cgagaacgcc tgcgcggcgg cggaaaaggt ccgggtcgac ctggagcttt tgcccgccta
    27001 catgagcgcc cccgccgcca tggcggagga cgccatggag atccatcccg gcacgcccaa
    27061 cgtctactac attcagaacc tggaaaaggg cgatcccacg gggcccatct tcgaaaaggc
    27121 cgatgtggtg gtggaagacg acttctacgt ggggcgccag ccccacctca ccgtggagcc
    27181 cgacgtgggg tttgcctata tggacgatga cggcaagctg atcatccact ccaagaccat
    27241 cggggcccat ctccatctct acatgatcgc ccccggcctg ggggtggagc ccgacaagat
    27301 cgccctggtc tccaacccca tggggggcac cttcgggtat aagttcagcc ccaccatgga
    27361 ggccctggtg ggagcggcgg ccctggccac ggggcggccc tgcttcctgc gctacaacta
    27421 cttccagcac atgacctaca cggggaagcg gtcgccgttc tttgtgaaca tgcgcttcgc
    27481 cgcagacaag gcgggaaagc ttttggccat ggagacggac tacagcgtgg accacggccc
    27541 ctattcggag ttcggagacc tgttgaccct gcggggggcc cagttcatgg gggcgggata
    27601 cgacattccc cacatccgcg gggtggggcg cacggtgtgc acgaaccatg cctggggatc
    27661 ggcctttcgg gcctacggat ccccccagtc cctgtactcc tccgagtccc tgatggacga
    27721 gctggcggaa aagttgggca tggatccgtg ggagatccgg tacaagaact gctatcgccc
    27781 cggggcgacg aaccccaacg gccaggctcc ggaggtcttc agctatcccg agatgctgga
    27841 ggccctgaag ccccgctacg aggccgccaa ggataaggcc cggcgggaat ccacagacag
    27901 ggtgaaaaag ggcgtggggc tctccctggg gtgctacggc tgcggcctgg acggccccga
    27961 cggctccgag gcctgggcca tcctcaacca agatgatacc atcactatcg ccacggcgtg
    28021 ggaggaccac ggccaggggg cggacatggg ggccatcggc acggctcacg aggccctgcg
    28081 tcccatgaag gtggccccgg agcgcctgcg tttcacctgg cccgacacgt cgaagacccc
    28141 caattcgggg ccctcggggg gctcccggca gcaggtcatg acggggggcg ccatccgggt
    28201 ggcctgccag gagatgctcg cggggctcaa gaaggccgac ggtacctatc gcacctacgc
    28261 ggaagccacg gcggacggcg ttcccacgaa gtacgtgggg aaattctccg tccccggggt
    28321 ggcctgcgac gacaaggggc agggaaagcc cttcgtggtg tacatgtacg gggtcttcat
    28381 ggcggaggtg gcggtgaatc tggacaccgg caagaccacg gtggagaaga tgaccctcat
    28441 ggcggacatc ggcaaggtga ataaccgcct ggtgacggac ggccagatct acggcggcat
    28501 agcccagggc atcggtctgg ccctcaccga ggactttgaa gacctgaaaa agcactcctc
    28561 catgacgggg gcgggcttcc ccttcgccaa agacatcccc gacgacatgg agatcgtcta
    28621 cttcgagggg tacccccggg agttcggtcc cttcggagcg gcgggggtgg gagagctgcc
    28681 gctgtccagc ccccacgcgg ccatcggcaa cgccatctac caggcctgcg gcgtgcgggt
    28741 gcacgccctg ccggccctgc cggaaaagat cctcgcgggg ttgaagggcg agcgggtcta
    28801 gcgttccaac tttggggccc ggaggcgtct cgcttcgggc cccaaagttt ttgccggagg
    28861 agaacaattc tgccttccgt catcttcgcc cacggcattt ttccggtgga ccggcgaggg
    28921 cagaaggggt aggttttttc ccgaagcgca taccgagagg gacaactttg gacgccggag
    28981 cggcagggaa cgctcctcgg cctttccgct tccgctgttt ccgacgaacg ccctccaggg
    29041 ccgtcttcgg cggcgcctct ttcgatgcga aaaaattttc ttgaaagcga cctttctccc
    29101 tacggggggc gtggattgcc atctcggagg tgggccatgg aacaggaaag cctcaaggag
    29161 ctggagcgcc gctgtgtgca ggaggagctc ccctactgtc aggcggcctg ccccctgaag
    29221 gtggatgggc gggccttttg cggcgccatg gggttgggga agttcgacga ggcccggaag
    29281 atcttgtccc gggccatgcc ctttcccgac ctggtggggc ggctctgcga cgccccctgc
    29341 gagaggagct gtctccgggc cgaaaggggc ggggcggtga ccctgggggc tctggagcgg
    29401 gcctgcctcg accgatcccg gccggggccc ccgccgatgc gcctgccctc ccgggggagc
    29461 tccgtgacgg tgctaggggg cgggctgtcg tctttggtct gtgccctgga gctggcgaaa
    29521 aaggggcacc gggtgaccct ttggggggcg gaaggccccc tgggggggca ctgggcccgg
    29581 gagggtgccc tgccccccga gctctgggaa gaggcggggc gcctgttgcg gcatctgggg
    29641 gtgcggtggg aaggggagcg aggcgttttt ccccccctgc ctccgggggg agcgctgtac
    29701 ggcggcgggg acgaccccgg gtggcgtggg gccctgccgg aggagtggga tcccatgacc
    29761 cacgccacgg ggaccccggg ggtctttgcg gggggagctc cccgtgcggg ggggccgtcg
    29821 gcggtggagg aggccgccga tggcaagagg gctgcccaga gtatcgaccg ctttctccag
    29881 ggggcctcgt tgctggcggg gcgggagaag gagtttcaga ccgagacccg gctgtttacc
    29941 tcgctggagg gggtggtgac ggagccccct cgagaggatc ccgtggagga ggcccgacga
    30001 tgcctccagt gcgagtgtcg ggtttgcgtc cgggaatgcg tctttctcgc gtcccacggg
    30061 cggtaccccc gggtctacgc ccgggagatc tacaataact tcgccgtggt gcacggatgt
    30121 catcaggcca acggcatgat aaacagttgc gccctctgcg gcctctgcga gaccctttgc
    30181 ccccacgatt tttccatggc cgatctctgc cggagcgccc gggaggagct ggtggcccgg
    30241 ggcatcatgc ccccgtcgac ccacgccttc gctctgcagg acctggccca ggcccaatct
    30301 ccccgggcct cggggtttcg ccccggcacc cggggcaccc gggcggtgtt ctttcccgga
    30361 tgccagctgg cgggggccct gccggagcgg acgggggctc tgacggcctt tcttgaccgg
    30421 cgtttcgacg gagccctggg ggtgcttttg gggtgctgcg gagctccggc gtggtgggcc
    30481 gcccggcggg acgccctcga tccggtggtg gaaaggctgg gggccacgct cttgtccctg
    30541 ggaaatcctc cggtggtgct ggcctgtccc tcctgcgccg acatgttccg gcgctttctc
    30601 cccgaggtgc cccgccgctc tctttgggag gtgctcttgg agggggaact tccccccgga
    30661 gctccccgaa gggagccctt ggcgatctcc gatccctgta ccacccggca cgatccggcg
    30721 tggcgggggg ccctgcgcca ggtgctggag cgtctggggc aggagtacga agagctcccc
    30781 atgggagggg agcgcacgaa gtgttgcgga tacggggggc tccagtccca gggggccccc
    30841 gagctggccc gccggggggc cgaggtgagg ctccgggaga gttcccggga ttttctcacc
    30901 tactgcgcca tgtgccggga gagtttgtcc ggcctgggga aaggggtgtg gcacgtgtta
    30961 gagcttcttt ttcccgagat gccccccgaa ccggccacgg ggttttccgg gcgccaggaa
    31021 aaccgaattt tgttgcggga gcgtctcttg ggggcttcgg ggattccccg ggagtcctgg
    31081 gaagatctgg agctcggggt gtctcccgag atgcggcgcg ggctggagga gcggcgcatt
    31141 ttggacagcg atctccggcg cgtgctggcc gcctgccggg aaaaggggag gggcctcgtg
    31201 gccgatgacg gccgggcgtt ggcgagcctg cgcctgggag aggtgacctt ttgggtggag
    31261 taccgtctcc ggggagaggt ctttgagatt ttgaacgcct ggagccaccg catgaccgtg
    31321 gaggttattc catgagcgag gcgtcttttt cctttcagga cgaaggactc tggcggtgcg
    31381 cctgcggcgg cacgctggag atgcggcgcg tggcggcctg ctatctggag agccgctttg
    31441 agattgagct tccggtctgc ggaaaatgcg gttttattct ggtgcccgaa tccctcgccc
    31501 ggggaaagat gcgggacgtg gaacgcattc tggaagacaa atgacatccc cctttcccga
    31561 ggagctcctg tggccccagg aactggaaga gaccctgcac cccggcggcg agggcacgtc
    31621 ccggcggttg cttgcgctct gcgcctttgt cccggggggg cgggtgttgg acgcggggtg
    31681 cggccggggg ggaatgctgg agcttt
//
LOCUS       DAOOER010000021        30978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_1690_length_30978_cov_9.927659, whole genome shotgun sequence.
ACCESSION   DAOOER010000021 DAOOER010000000
VERSION     DAOOER010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 30978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30978
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1690_length_30978_cov_9.927659"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="PL162_04490"
     CDS             <1..562
                     /locus_tag="PL162_04490"
                     /inference="COORDINATES: protein motif:HMM:NF020968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HQF91311.1"
                     /translation="FRGHKARAITGQKERAATGDVWAQNRGAYLELPGTGQWKGLAAW
                     LAPRTDIFFAHLTSRYMLFGEWCYARHSVEYDQLPSWFLGFDVYDKVSGRFFSSPRRD
                     AWFQALRLDQVPRLGHGRFTIAGLGEFLSQSRLGDKPMEGLYLRSDQGDWLVQRAKLV
                     RHDFIQSVEQHWSRSGIKPNRLLRKA"
     gene            complement(618..1313)
                     /locus_tag="PL162_04495"
     CDS             complement(618..1313)
                     /locus_tag="PL162_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQF91312.1"
                     /translation="MREPENKKHKILNRAWRLFLRHGFTRTSMRAIAEDAGVSLGLLT
                     YYFTTKDNIALELLEKQIADFKAALNRVIDLDEDPVLYSASLVRINYKVMSMPEFRVF
                     YLDAMRNDLYFKTIPNRRLFPDRGLESLSRINDKYRLGHSPEYLELFGNYLCVSMERT
                     LVLYPDILEAMGHLPDLVFRTYMGHLDPHFRDFDAYCQRSEAAVQRVLREHPEVRTVH
                     DLFDEGPEGPEEG"
     gene            complement(1610..2950)
                     /locus_tag="PL162_04500"
     CDS             complement(1610..2950)
                     /locus_tag="PL162_04500"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HQF91313.1"
                     /translation="MSQVKSTKELDRVLGRKDLMSIAIGQTIGSGIFALMGVGLAMTG
                     RSASLSMIVGALFVILISIPNIFVGGTIRMRGGFYTQLGLLVNKTCSGFFVIVHILAW
                     TALALYALSFADYTLAMIPGINKTLVAFSVLTFFYVVNLLGIQSAARVQNLMTLAMVV
                     ALTVFICFGLPQVQPGYFEGPDFMTGGWQGLLAAGALYTWAIGGANVIVYLGAEAKNP
                     TKDIPFVIIVATLVIALFYALMATVAAGVLPISEVANQPLSVVAAKILPRPVYIFFIV
                     GGAMFALTTTLNAALGWVTKPILQACVDGWLPSGLGAISKKYKTPYIILTLLYLESLV
                     PIFFEFNISTIGNMAVILNNVLFAIICFSAIRMTAVIPEIWGQSKFHVSKGTLAFWSI
                     LGGVATLGQTALLFSVLKPYEMVGNVVVVVFALGYAMLRMRSGKVNMEVSYEEV"
     gene            complement(3089..5062)
                     /locus_tag="PL162_04505"
     CDS             complement(3089..5062)
                     /locus_tag="PL162_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQF91314.1"
                     /translation="MAMVVKKFPHLFSPIRIGSLVARNRIESAPISMGDLTPEGYLTR
                     ENVAAYEVKARGGAAIVNIGESNVHTRTGKAHGRMVPLDDEEVLPSLINTTDAIKRHG
                     AIASIELIHPGRRANPRYFDGDTYGPTGGPGLFGKPVIELTEELIEEIVEAFGDAAEM
                     ARLGGMDMCMVHAGHGWLVHQFFSPLNNQRTDRFGGSLENRVRFAFMIVDNIRKKCGR
                     DFPIEFRISGSELTPGGFDLDDMMEFARMADGRIDLLNVSDGTFHVPSTNTTMVPSMF
                     LPHGCNVYLAEGIKKAVKKTPVSALGGIGDPEMMEEIIASGKADMVALGRALIADPDL
                     PRKAKTGRADDITPCQRCIVCMSGSFVPYVKYPTRVGKCTVNPTIGKEYELFTSVPSE
                     GRKRVVIAGGGPAGLQAAITAADRGHEVFLFEKSGSLGGAIQFAEHVPFKADLKKFMD
                     VLIRRVERRAITVHLNTLLTPDLARELRADVIIAAVGADPIVPSLPGIDGPHVVRAVD
                     LHDEGVTVGPRVVFIGGGLVGCEEGLDLAQRGHRVTVLEMRENVAIDAAYLHREALLL
                     EIAKHPEAITLLTHMRCKEVTLEGVVAVDEAGTTRFFEADTVVVAAGMRPRSEAVESL
                     RDCAPDFLVIGDGRKPQRVLEAVRSGYDAGMHL"
     gene            5517..7514
                     /locus_tag="PL162_04510"
     CDS             5517..7514
                     /locus_tag="PL162_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQF91315.1"
                     /translation="MAYEKLFEPMRIGNVELKNRISMAPMGLTGLATPQGGFEERAVE
                     FFVERARGGAGLLITGTVKVENDIEPFPMPSQPAPLVNPAHFRLRALELTERVHAFDC
                     RIFLQLTMGFGRVAPPTLVPRPVAPSPIPNYWDPSILCRELDIDEVTRLIDAFGASAL
                     IARECGFDGIEVHAVHEGYLLDQFALACCNRRTDKYGGDLRGRLTAAREVRGAIARYA
                     GEDFPVMLRYTLKSFMKGWRQGGLPGEEFQEFGRDMAEGLEAGKILEESGYDAFNADA
                     GTYESWYWAHPPVYLEEGCYLPYTEALKGVVSVPVIAAGKLGDPDRAERALREGRADG
                     VSLGRPLLADPAWPLKVRRRDVASIRPCIGCHDACMNRIAKGKPLSCTVNPATGRERE
                     FALTPARERRRIAVVGGGIAGMEAARVAALRGHAVVLFEGTGELGGHVIEGSVPEFKR
                     DDRRLLAWYRRELERGHVDVRLHHRGDRAVLDAVNPDAVVVATGSLPLVPPLGKRREG
                     APEVLTAQEALRGERPLGRRLVVLGGGFVGCELAVWFTQQGHEVELVEVREGLMTTGD
                     VPHPNRIMLLDLLTFHRVGVWTGARLSLLAPEGATIQSADGERLLPCDTLVLATGYRS
                     DRGIWQEVSGAFPEAYLVGDALRPANVQHAVWTAYEVARQL"
     gene            complement(7634..8554)
                     /locus_tag="PL162_04515"
     CDS             complement(7634..8554)
                     /locus_tag="PL162_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HQF91316.1"
                     /translation="MRLLTRSDFDGLMCAVLLKELDLFDERKFVHPKDIQDGLVEVNE
                     KDILVNIPFVPGCGMWFDHHSSEEERGLMNAYSFRGAAWPAPSCARVVYEYYGGDQGP
                     LGHLKDVVAMADKCDSAQFTRDEILNPSGWVLLSFVMDPRTGLGRYRDYRISNYQLMD
                     DLADLLRTHSIEEILGLPDVQERVRRYFNQEALFREMILAHSWTQGNAIVTDLRGIPE
                     TFVGNRHMVYALFPEQNISVRVFDGREKAFCVFSVGHSILQRTSHTNVGALMLAYGGG
                     GHPRVGTCQVPYEEADRVLREMLAVINRDG"
     gene            complement(8754..9287)
                     /locus_tag="PL162_04520"
     CDS             complement(8754..9287)
                     /locus_tag="PL162_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91317.1"
                     /translation="MMHIKDLMQRGALCKGDVVIYKWLKEEIQAKDFFAPLRMVEFFM
                     GFMISQVRDGRIYDALGPFPQENVVAVFKPIMAVDKSLLVWKMSEERMCTETLSLLIN
                     RLLDVFPEAYLFQDETYFEILRRRSLTHYDLVEVGAELVAQLERGRYREEGKDVVGKF
                     NQIVAHRSPGGDPHRRR"
     gene            complement(9611..10630)
                     /locus_tag="PL162_04525"
     CDS             complement(9611..10630)
                     /locus_tag="PL162_04525"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQF91318.1"
                     /translation="MTPLYELQGVVRRYGDRVALDVPELAIHPGEIVGLVGPNGSGKS
                     TLLRTLAFLDPPDEGMLLFRGAPACPATPELRRQATLLLQEPYLLKTSIFENVAYGVR
                     LRGEKDPVGRVRKALEAVGLDYDAFARRPWHALSGGEAQRVALASRLALRPAVLLLDE
                     PTASVDASSAGAVRNAILSAVRQDGTTLVVVSHDLLWLYEVASRTVGLRGGRVVASGA
                     ENVLAGRWERKEGEPGHLVLEDGQGVMAPPMPLGATMGLLSPEAIALSILPPGGSAPR
                     NVLKGTVGQMFWERGPHRVLVRVGVGSLSLAVRMTADAVRDQGIHPGREVWLSFDPSA
                     LRWGS"
     gene            complement(10627..11322)
                     /locus_tag="PL162_04530"
     CDS             complement(10627..11322)
                     /locus_tag="PL162_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQF91319.1"
                     /translation="MDYIAQGVIQAFVLLWTGDEETWSAIYTTVKMSSISIAVCLALG
                     TPLGFILGYADFPGKKGVRTVVNTLLALPTVAVGLVVYAFISRRGPLGELGLLFTVSG
                     MAVGQIILALPVVVALTANAVESMDERLRLTLLTLGADGKRLALSTLYEARYAVLLAA
                     ANAYGRVISEVGVSMMLGGNIKWHTRTITTAISLETGKGEFALGIALGLVLLILAFAV
                     NSVLSLARQRATI"
     gene            complement(11411..12235)
                     /locus_tag="PL162_04535"
     CDS             complement(11411..12235)
                     /locus_tag="PL162_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HQF91320.1"
                     /translation="MKRTMRILSLVTLVLMLATAAFAAPVLRMATTTSTEDTGLLDYM
                     EPFFRQATGIELQWVAVGTGKALELGKNCDVDVLLVHAPSAEKIYVQEGHGVNRRQVM
                     YNDFVLLGPAADPAKVKGLAVGEALKAIAAAKQPFVSRGDKSGTHVAELVLWKGAGME
                     APDKDAWYISTGQGMIQTINVAGEKNGYTLADRGTFIKYEANAKGNPPVVILVEGDSA
                     LRNQYSVIAVNPAKCANVQYDLALKYADWMVSPETQKLIADFKLEGKQLFFPNADE"
     gene            complement(12318..13253)
                     /locus_tag="PL162_04540"
     CDS             complement(12318..13253)
                     /locus_tag="PL162_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HQF91321.1"
                     /translation="MGVLLAAAVILGAAGVSGAKETLTVWHAGSLNAALKDVEARFLE
                     KYPDLEIQDKGAGSVELAQAIVDKGGCDLYLTADYRNIEDLLKPQHATWNLLFCTNAM
                     VIAYSPKAPRAGEISAANWFRVLARPDVAWGHTDPNKDPGGYRAIMVLQLAEAFYGQP
                     GLEKSLMEAPGHRVVDGINAKGVKKLMEWGSLGYMLKYRSYAVADGMQMIDLPAEINL
                     GDPTFAENYAKASVEIKGKGDEKITMRGEPVVYGLTIPTSAKNGARALEFVQFLLSPE
                     GKQILEKRGFGLMTPAPATPRDKLPAEIAALTAAQ"
     gene            complement(13332..14162)
                     /locus_tag="PL162_04545"
     CDS             complement(13332..14162)
                     /locus_tag="PL162_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HQF91322.1"
                     /translation="MALERERDGKRLHVKSRLMGESLVSRSFAEQLETAPQVRLFPDV
                     HVLKIGGQSLCDRGAKALPALAREIVENKARHKMLFTTGGGTRSRHIYAIGLELGMPT
                     GIIAKFGSSVSEQNALLVATLLAPWGGLKIGHDEIVKLATYFGQGCIPVMHGMPPYDY
                     FALPPEKGRIPLHRTDVGTLLLAELIGAKSCIFLKDERGLYTDDPKKNPGAEFIPEIS
                     VAELRELDLDDLVLERPCLEILERSEVLDRILVVNALEEGNLTRALDGEAVGTVIRRK
                     "
     gene            complement(14165..14914)
                     /locus_tag="PL162_04550"
     CDS             complement(14165..14914)
                     /locus_tag="PL162_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91323.1"
                     /translation="MRWFVGMDDTDVVGAPQGTGKVARFFGGALPEGWRCLGVVRQQL
                     LVDPRIPYTSHNSAACVVLEGEGEKEKPGELLATLGAAHLGAFALPGSDPGLCVAPEG
                     HPALKLLGDFGERALREVLSQDDAREAAREVHLSAHGGTGDGIIGAAAAVGLTAAGWR
                     GRCISWGDLRELPEAVPVEELERRGLRVVWLERDAFSVPPGALIRGGGWFRPFLLGGS
                     PVLLVHREEDGTFRTVPGKERIQRNDRKREG"
     regulatory      complement(14997..15116)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(15249..15503)
                     /locus_tag="PL162_04555"
     CDS             complement(15249..15503)
                     /locus_tag="PL162_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91324.1"
                     /translation="MAGTIFYRERRKVGEQDRQPRFRVVAVAGAEIHFFATHLRLQEL
                     ESMAQAVGATLVALPAGEGHEGGHGGRRGCGGHRGGTPCS"
     gene            15731..16816
                     /locus_tag="PL162_04560"
     CDS             15731..16816
                     /locus_tag="PL162_04560"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HQF91325.1"
                     /translation="MRQRLLVCLAVLGILGGAVSASAEGLFRGDTANTGIFPVSRLPQ
                     GVLAWQIPTSARIAGSPVVAGGTVFFANAAGELVASDLRTGQGRWALRLDGSVLGSPA
                     VAGQTLFVGTYEGTIFAFSTSRGTTLWHASTKGAIFGAPAVVGSTVYVGNALGQLRAF
                     DAASGRERWVFTGNGWFAGTPAAGEGLLFVGDHGGRFRAIDPARGTEVWGANAGGSVD
                     PSPVVSGGWVFYGSDDGRIAAVQIKKVIPKWQVKLGAGIASSLGVAGGLVFAGCHDGQ
                     VYALDARTGAVRWTFLTEGPVLSSPAYARGTLYFGSSGGTLYAVDAASGRELWRFAAG
                     SEISGAPVVAEGMVLFGTADGTLFAVR"
     gene            16983..19004
                     /locus_tag="PL162_04565"
     CDS             16983..19004
                     /locus_tag="PL162_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQF91326.1"
                     /translation="MRFVMAMMALWLFLGSVSAGEGALPLPPVPADSYDVVVVGAGTG
                     GCAAAISAARLGMRVALVEESTWIGGQLTASAVSTMDDVRRNRSGLYGEVISRIVAHY
                     KALGRTVNTCYWGSDTIACEPAVGQKILSDMIAEAQGGGPEGTGRVELFPETRVTSAH
                     MEGFRLVAVKGERNFRPLVFRGKVFIDATECGDLIPLTGARYRVGNCVAPHIDPEANV
                     QDITYVAVIRRLSGDLPPELRIATPPPGYDDHVEDFRILVTKDGSPKPGTKPVNPVVH
                     NAYRGLPDPENPLSVDCGRANTWPNISKTGINWANDVPGESGDQPGLSVRYIEDRLFR
                     KAVDRQALLRTLQFLYYFQTEAGASNWTVDTTQGFGSFFSCNWEVWKDMPPEYASVLR
                     HFPPTPYVRESRRIVGLRTLTAADVERNEKTGMAFRNFPSSVALGEYPVDIHGSHLDR
                     YLEHDLGEFEHTFPKKWRPSKGVFQVPLEVLIPEQVDGLVAAEKNISVSRMVNGATRL
                     QPITLLTGQAAGTLAAVAVQRGVEPRRVKPLWVQNALWQRRSRLAMEIWQDVPIQSDW
                     WPAVQAASLYDTMPSLGKKIFGADFPMKRRHAMELLRRAFPGHFGMDTSAPSMEFFIT
                     RGEFEILLRMGLSAEMPLPPTAVYQDDLRGEITRGEALARTLEVLLAQH"
     gene            19149..20354
                     /locus_tag="PL162_04570"
     CDS             19149..20354
                     /locus_tag="PL162_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQF91327.1"
                     /translation="MIPRSLVERMFSAESIERWNDHPRPLHFTEMAKQAHKFMLAWLI
                     ARNEEENGKSVDWLALVEGVLFEFLHRVVLTDIKPPVFHRMMAQHGEKINAWVAQELK
                     DDLAPLAGGFYGRFQGYFSSTAPETPVALERDILRAAHYLATRWEFGLIRAWGAPLYG
                     IDETGREIDAEIERYRHLRAVDLLMVASAKGDRLGLPAFIDLVGQLRFQKRWTQTPRI
                     PETSVLGHLFFVAALTYFVLGETGLPRNVVVGTVLTGLFHDLPEVLTRDIISPIKRAV
                     EGLEDFIKDYEQDVMEEKIYRRMALPEGCVRDLRLFTRNEFQDRLRHPDDRVTPYAPE
                     NADSGWPVLGSVVEACDKFAAFVEASLSIRYGVSTAQLQTAKQRLAEKWSRCVIHGYE
                     LRPLYDYFS"
     gene            20392..20949
                     /locus_tag="PL162_04575"
     CDS             20392..20949
                     /locus_tag="PL162_04575"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HQF91328.1"
                     /translation="MKRALLVIDVQNEYFDGALPVTYPHGSFDHILRALGAAREAQIP
                     VVVIQHSATSPESRTFRPGTPGWELHPSVAAHPRDLLLEKTLPGSFTNTELESWLRER
                     QISTVTIAGYMTQMCCDTTSRQAFHRGFHVEFLSDATGTLAFQTTVGAVTAEELHRAI
                     LVTQALRFAEVLSTDAWIARLQSGD"
     gene            complement(21175..22179)
                     /locus_tag="PL162_04580"
     CDS             complement(21175..22179)
                     /locus_tag="PL162_04580"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQF91329.1"
                     /translation="MRWATGFLVAVFLMTCGTTSLAGQQRDMGELQKAAEGGKVEAQF
                     EMGLAYWSGDGVPVNEPEAAKWFGKAADQGHPLAQAYLGLQYFEGAGLPKDEARGQSL
                     AGAAKDAVVRMAEEGNVAAQDVLGELFRGGIGVPQDDAKAVEWYRKAAEQGNADAQFE
                     LGVMYANGQGVSQDDMKAMEWFRKATEQGNAFVQWRLGAMYEFGLNVSEDFTKAVDWY
                     RKAAEQGYAPAQRSLGSMYREGRGVPQDDTKAVDWIRKAAEQGYALAQGNLGEMYQWG
                     RGVRQNDKEAYRWYYTAGVLGDADAAERAKRIVVSVSAEDAARIRREVDEKIRRQGWK
                     "
     gene            complement(22935..24437)
                     /locus_tag="PL162_04585"
     CDS             complement(22935..24437)
                     /locus_tag="PL162_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91330.1"
                     /translation="MLLVLGVLLGAGGAGASDLTIDLTPGWTMGSPDAGDMVRCDLTL
                     EPGKTAAVFYVKRLDFDTVEEAAGFGKYLREQMRYDDNTTLLKNAEGLVSGIPSVVHL
                     VVERRQSGTYQREVSYVPLEKAWFQFGVLTTPELFAQVKLRSDELLSRVRLVGGTAWQ
                     GGTGASGQGPVSGGVAGQGGTGVSGQGTVSGSTAGTGVAGLAGGSSGGTPRLTFNVPP
                     PWVPGNPPDPSVKASFLLPEGSQVRAELLVFEEAFTDGNVDLYFQRVQAYAATAFAAF
                     ESLGTAAVPLAGREGRRLDFRFSPAGGTNRLRGRFFALVERGRAFGFLFDCGAQDFDA
                     LASAFDGIMTSVSFADEGGAGTGGSVGTTTGPAPAGVYTDAKGLYTVPLPPGAAFQES
                     LLFGAIYSLPAGGQISFFTFEGEEPVANLQAQVASGKTPHGESRIAARGKEALVTLYS
                     SVNAETKVAYATLFVRFPGTGALVIVSVPAARYQESQGWILPFIQSFQFR"
     gene            complement(24733..27705)
                     /locus_tag="PL162_04590"
     CDS             complement(24733..27705)
                     /locus_tag="PL162_04590"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HQF91331.1"
                     /translation="MKTLCLMAVLLWLAGGAAGETPGAPPLSPDGGGLLGSAPGPEEV
                     DRRREELRRRIMALVYQGRWHDAEAEAWAFRLLPSSTGGARERGEELVVRGYVLSRRG
                     RDAEARGAADRAAEFLEQGKDREGLLRALAVAIRASQGMGDWNGSRERLDRLQALARA
                     ASSDYGASVGALEEGIWAYHRGNVPGAVAFWERAFSLGKNLDNPEIRGLAANHNARGR
                     YLLGRHDEARQWFDRALDEARGAPWLEFVVRLNRAEFLAEGLFNSAGALGDLQRAEKL
                     AVSLDLGSGRLWAWNNLGTFFLARGEYGKASEYLEKALDFQMGHDVPEDVGVLINGAT
                     LRQELGQEAEADKLLRRALELAQERGDVRSQPGILGNLGIVARRLGYLAEAEGYYRRA
                     LELCQRLGMDRQGADLLVNVANLNVEMGAWDNADKALGEALREYEARGDLVGEALVRA
                     NRGYARCAAGRRALGVEDLLRSVEIARRVEHRPLIFFSLLEYARELVASGDRHRALPL
                     LEEAAGVLERSRSELASVEHRMAFMERRYEVYDLLMDLYVRSGRFAEAFDAAERMKAR
                     AFLDVVGGGTVKLLPKDRDLVGAITALEGEVEVLSRKKSDLLAASAPVPRVLEDRLRT
                     LRDKLAFLRSELSLASPELLSLVAVRPLSLDRIQKSIPEGTLVADYHLLADRVLVFLL
                     SRNEFSCRELPLLSRDAVSSAVRAVAPALKDYKAEIPYVELSRLYLGLVAPLEEPFRK
                     AERVVVVPQGVLSYVPFQAFWDGEGFLVRRFAISYAPSLSVYALLAAREGPTPKNLVG
                     FGNPAFADAAVPPLPFAEEEARQVAALFPRSQLLIGGDATESALYARAPEADVLHLST
                     HAKADPKRPLNSVILLAPGGGQDGRLTAGEVFGMNLSADCVVLSACETALGSMNHQEG
                     LVGFSRSFFHAGASSLFVSLWSVADEATRDLFVAFFSRWLGGMSKSRALQEAQMAVLS
                     RTNHPMFWAPFILLGREQ"
     gene            complement(27716..28897)
                     /locus_tag="PL162_04595"
     CDS             complement(27716..28897)
                     /locus_tag="PL162_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91332.1"
                     /translation="MKISFWSDGARKSGRRALGALAAVALLAGGAGAADSVAHPLIFG
                     GNVLGAPALRGGAGAPEVPSGVEVRLATGDVIVFGVPSEAWRRRGGPSLLTPLEEWGT
                     RALPGALRGGAEGTLPSLPTTPDAGAAGGTGTLPSLSGAFAPAPSPATSGGGQLPPLV
                     GGQDTGGATGNPPLVGGQGTGGLLGNPLLAAPSPAPLVGGTGGLGALGQTPVGDLAVW
                     KTFDLSTAFGFSQFPLGRLEYPGKWMVQVDTFNRTVTFAEDASGMVCFTIAPGATVQV
                     SSARDLAIQVATLLTSNVPDLAVVREEFREDPTFQGSGYALTFGRFLLAGTFQGRAMM
                     AMVQTYVMASQMSGGMPFALGTAMVCTAPREVFDQRLQSVFNRMITSYEASVSKPSKK
                     D"
     gene            29188..30483
                     /locus_tag="PL162_04600"
     CDS             29188..30483
                     /locus_tag="PL162_04600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D C-terminal
                     domain-containing protein"
                     /protein_id="HQF91333.1"
                     /translation="MGNGSKPLRLLHTSDWHIGHAIYGRKRYAEFGAFLDWLGEILVR
                     EDIDILLVAGDIFDSSVPSHRAQELYYRFLRRITPSSCRHAVLVGGNHDSPSFLDAPR
                     ELLQSLSVHVMGAPGARREDEVKILRNPQGHPELLVCAVPYLRDGDLRTAEAGETPED
                     KDRKLVEGLRAHYAQVAALGEEIRQREAPGVPLVALGHLFVAGGTSAEGDGVRELYVG
                     SLARVSEDIFPQNLDYVALGHLHAPQRVAGAETRRYSGAPLAMGFGEATQQKSLCRVE
                     FSGRRASVDLLPVPVFQRMERLRGSLEEMLSRLRNLAAEGKSVWLEADYDGEEIQGDL
                     RSSLEEAVSGTALDLLCVKNRRLPACVLFGEEEDGALADLNENEVFERCLEEASVDES
                     QREELRRAYREILLSLAQDDPLGEERSGACASSTSTSKI"
     gene            30450..>30978
                     /locus_tag="PL162_04605"
     CDS             30450..>30978
                     /locus_tag="PL162_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQF91334.1"
                     /translation="MRILHIHFKNLNSLQGEWTVDLTHPAYVSEGIFAITGPTGSGKS
                     TLLDALCLALYGATPRLGRITKTTNGIMSRQTGECFAEVTFETPQGCFRCHWSQRTAR
                     KKAGGELQQARHEVIDHATGNVLEEKLSEVPRRVEEITGLDFRRFTRTMLLAQGDFAA
                     FLQASSDERGPLLEQI"
BASE COUNT         5391 a        10628 c         9624 g         5335 t
ORIGIN      
        1 atttcgcggc cacaaagcga gggcaattac cgggcagaaa gagcgggccg ctacaggaga
       61 cgtctgggcc cagaatcgag gagcgtactt ggaacttccc ggcaccgggc agtggaaggg
      121 attggcggcg tggttggctc caagaaccga tatctttttc gcacacctga ccagccgcta
      181 catgctcttc ggagagtggt gctatgcgcg gcactcggtc gagtatgacc aattgccgag
      241 ttggtttctc ggattcgacg tctacgacaa agtctccggt cggtttttct cttccccccg
      301 ccgagacgcg tggtttcaag ctcttcgctt agaccaagtt ccgagattag gccatggccg
      361 ttttaccatc gcaggcttgg gcgaatttct gtcgcagtca cggctcgggg acaaaccgat
      421 ggagggactc tacctccgat ccgaccaggg cgattggctt gttcagaggg ccaagttagt
      481 tcgccatgat tttattcaat ccgtcgagca acactggtcc cgttccggca tcaaaccgaa
      541 taggctcctg cggaaggcct aggcatgccc cccctcgccc ccggcgcccc gataagatcc
      601 ccccgcagag ggcgccccta cccctcctcc ggcccttccg gcccttcgtc gaagaggtca
      661 tgcaccgtcc gcacctcggg gtgctcccgc aggacgcgct gcaccgccgc ctcgctcctt
      721 tggcagtagg cgtcaaagtc ccgaaaatgg ggatcgaggt gccccatata ggtccgaaag
      781 accaggtcgg gcaagtgccc catggcctcc aggatatccg ggtagagcac cagggtccgc
      841 tccatgctga cgcaaaggta gttcccgaag agctccaggt actccgggct gtgccccagg
      901 cgatacttgt cattgatccg cgacagactt tccagccctc gatcgggaaa gagtcggcgg
      961 ttggggatgg tcttaaagta gaggtcgttc ctcatggcgt ccagataaaa gacccgaaac
     1021 tccggcatgg acatcacttt atagttgatg cgcaccaggg aggccgagta gagcacggga
     1081 tcttcgtcca ggtctatgac ccggttcagg gccgccttga aatcggcgat ctgtttttcc
     1141 aggagctcca gggcgatgtt gtctttcgtg gtgaagtaat aggtgagaag ccccaggctc
     1201 actccagcgt cttcggcgat ggcacgcatg ctcgtcctgg tgaagccatg gcgaaggaag
     1261 agccgccagg cccggttcag gattttgtgc tttttgtttt ccggttccct catcgtcgcc
     1321 tcccccgccg ccttcccatc gtccccgcag ggaccttcgc gcgcgcccgg ggcccttccc
     1381 ggggcgcctt tcgcgcccct ccctccgggg ccccggaggc tcccgcgatt ataatgccct
     1441 ttttcggaag acacaacgga cccgggggcc cggggcgctc ctcctccggc ggcgcggccc
     1501 ccgccccgtc ggaggaggag cttccgaaag ggccgggggc cgactcatcc tcgcctcggt
     1561 gcggcgagag cggatctttc gttgcgaagc ttccgccggg ggtcccgggc taaacctcct
     1621 cgtagctcac ttccatgttc accttaccgc ttctcatccg aagcatggcg tacccgaggg
     1681 caaagaccac caccaccacg ttacccacca tttcgtaggg ctttaatacg ctaaatagca
     1741 gggcggtctg tcccagggta gccacgcccc ccaggatgct ccagaaagcc agggtgccct
     1801 tcgagacgtg gaacttggac tggccccata tctcggggat cactgcggtc atgcggatcg
     1861 ccgagaagca gatgatggca aagagcacgt tgttcaggat caccgccatg tttccgatgg
     1921 tggaaatgtt aaactcaaaa aagatgggga ccaggctctc cagatagagc agcgtgagga
     1981 tgatgtaggg ggtcttgtac tttttgctga tggcccccag gcctgagggg agccacccat
     2041 ccacacaggc ctgcaggatc ggcttggtga cccatcccag ggcggcattc aacgtggtgg
     2101 taagggcaaa catggcgcct cccacgatga agaagatata gaccggccgg gggaggatct
     2161 ttgccgccac caccgagagg ggctggtttg ccacttcgga gatcggcaag acccccgccg
     2221 ccacggtggc catgagggcg tagaagaggg cgatgaccag ggtggccacg atgatgacga
     2281 aggggatgtc ttttgtagga tttttggcct cggctcccaa gtagacgatg acgttggcac
     2341 cgccgatggc ccaggtatat agcgccccgg cggccagaag tccctgccat ccgccggtca
     2401 tgaagtccgg gccttcgaag taccccggct ggacctgggg aaggccgaag catatgaaca
     2461 ccgtgagggc caccaccatg gcgagggtca tgaggttctg gacccgggcg gcgctctgga
     2521 ttcccaggag gttgacgacg tagaagaacg tcaggaccga gaaggctacg agggtcttat
     2581 tgatccccgg gatcatcgcc agggtgtaat ccgcaaagct cagggcgtag agcgccaggg
     2641 ccgtccaggc caggatgtgc acgatgacaa aaaaaccgct gcacgtcttg ttgaccagga
     2701 gccccagctg ggtataaaag ccccccctca tgcggatggt cccaccgacg aagatgttcg
     2761 gaatggatat gagaatgacg aagagggccc ccacgatcat ggaaaggctg gcggatcgcc
     2821 cggtcatggc gagccccacg cccatgaggg caaagatgcc ggagccgatg gtctggccga
     2881 tggcgatgga catgagatcc tttcttccga gaacgcgatc cagttccttt gtggacttca
     2941 cttgggacac aacgaacacc tccaagggtt gtggtgactg gcgtatcgtt tcaaaaggcc
     3001 cgatttcttc cgtcccggtc ccccgatggc cgcagaaagg ccccagggag gatcaaaagc
     3061 cccttacgcc ccgggcccgg aggatccatc ataggtgcat ccccgcgtca taccccgatc
     3121 gcaccgcctc gaggacccgc tggggtttcc gcccgtcgcc gataaccagg aagtccggag
     3181 cgcaatcccg gaggctttcc accgcctcgg atcggggacg catgcccgcc gccaccacca
     3241 ccgtgtcggc ctcgaagaaa cgggtcgtcc ccgcttcgtc caccgccacc accccttcga
     3301 gggtgacttc tttgcaccgc atgtgggtga gcaaggtgat ggcctcgggg tgtttggcga
     3361 tctccagcag cagggcctcc cggtggaggt aggccgcgtc gatggccacg ttctcccgca
     3421 tctccagcac cgtcaccctg tgcccccgct gggccaggtc gagcccctcc tcgcacccca
     3481 cgagccctcc gccgatgaag accacgcggg ggcccacggt gaccccttcg tcgtgaagat
     3541 ccaccgctcg caccacgtga ggcccgtcga tgccgggcag cgaggggacg atgggatcgg
     3601 cccccacggc ggcgatgatc acgtcggccc tcagctctcg agccagatcc ggcgtcagga
     3661 gagtgttaag gtgcaccgtg atggcccgac gctccacccg gcggatcagg acatccataa
     3721 atttcttgag atcggccttg aagggcacgt gctcggcgaa ctgaatggct ccccccagac
     3781 tcccgctttt ttcgaagagg aagacctcgt gcccccggtc ggcggcggtg atggcggcct
     3841 gcaggcccgc tgggcctccc ccggcgatga ccacccgctt gcgcccctcg gagggcacag
     3901 aggtgaaaag ctcatactcc ttgccgatgg tggggttcac ggtgcacttg cccacccggg
     3961 tgggatactt gacgtagggg acaaaggagc cgctcatgca gacgatgcag cgctggcagg
     4021 gcgtgatgtc gtccgccctc cccgtcttgg ccttgcgggg aaggtcggga tcggcgatga
     4081 gggcccgccc cagggccacc atgtcggcct tgccgctggc gatgatctct tccatcatct
     4141 cgggatcgcc gatacccccc agggcggaca cgggggtttt cttcacggct tttttaatac
     4201 cctcggccag atagacgttg catccgtggg ggaggaacat ggaaggcacc atggtggtgt
     4261 tggtggaggg gacgtggaac gtgccgtcgg agacgttcaa gaggtcgatg cggccgtccg
     4321 ccatccgggc aaactccatc atgtcatcga ggtcgaaccc cccaggggtc agctccgagc
     4381 cgctgatgcg gaactcgatg ggaaagtccc ggccgcattt tttcctgatg ttgtccacga
     4441 tcatgaaggc gaagcgcacc cgattttcca ggctgccacc gaagcgatcc gtgcgctgat
     4501 tgttcagcgg ggagaagaat tggtgtacca gccagccgtg ccccgcgtgg accatgcaca
     4561 tgtccatgcc cccgaggcgg gccatttccg ccgcgtcgcc gaaggcctcc acgatctctt
     4621 cgatgagctc ttcggtgagc tcgatcacgg gcttgccaaa gagccccggc ccccccgtgg
     4681 ggccgtaggt gtcgccgtcg aagtagcgag gattggcccg acgccccggg tgaatcagct
     4741 cgatggaggc gatggcgccg tggcgcttta tggcgtcggt ggtgttgatg agggagggca
     4801 acacctcttc gtcgtccagg gggaccatgc gcccatgggc cttccccgtc cgggtgtgca
     4861 cgttgctctc gccgatgttg acgatggcgg cgcccccccg ggccttcacc tcgtaggcgg
     4921 cgacgttctc ccgcgtcaga tatccctcgg gggtcagatc tcccatgctg atgggggccg
     4981 attcgatccg gttacgcgcc accagagacc cgatccggat ggggctaaac aggtggggaa
     5041 atttttttac gaccatggcc atgacgaaag actcctcctc tcccttttgg agcccgtgga
     5101 gacgcagccg ccccggcgct gacgcgcctt cggaccccgc gtcgccatgg ggcacgacaa
     5161 gacacggaaa gccaaggccc catcactcga ggatgggcga tgacttcgat ttggaaatcc
     5221 tgcgcaggag gaactgtaca tgtacaaaga cattctcttg tacatgtaca gtcttgtcaa
     5281 gccctcttgt accctcggag taaaaaaact tctccttagg gtacttcggt tcgccctact
     5341 cccccggggg cgagggggca aaggccccac tccccttgac accggagggg ctccgagcca
     5401 ccatggggga aaggggcata tccgaagggg gaagaccgcc ccgccccgtc ccccggactc
     5461 cccgggccaa aagggccccc cttcggtgag agtcgatgga aggagcaatg gataaaatgg
     5521 cctacgaaaa gctttttgaa cccatgcgca tcggcaatgt agagctaaaa aaccgcattt
     5581 ccatggcccc catgggactc acggggctcg cgactcccca gggggggttc gaggagcggg
     5641 cggtggagtt cttcgtggag cgcgcccggg gaggggcggg gctcctcatc acggggacgg
     5701 tgaaggtgga aaatgacatc gaacccttcc ccatgccctc ccagcccgct cccctggtga
     5761 atcccgccca cttccgcctc cgggccctgg agctcacgga acgggtccac gccttcgact
     5821 gccgaatttt cctccagctc accatgggat tcggccgggt agccccccct accttggtcc
     5881 ctcgccctgt ggccccttct cccataccca actactggga cccttccatc ctctgccggg
     5941 agctcgatat cgacgaggtg acacgcctca tcgacgcctt cggcgccagc gccctcatcg
     6001 cccgggagtg cggcttcgat ggcattgaag tccacgcggt ccacgagggg tacctgctgg
     6061 accaatttgc cctggcctgc tgtaaccgtc gcacggacaa gtacgggggc gatctccggg
     6121 ggcggctgac ggcggcccgg gaggtccggg gggccatcgc ccggtacgcg ggggaggatt
     6181 ttcccgtgat gctgcgctat accctcaaga gcttcatgaa ggggtggcgt cagggggggc
     6241 ttccggggga agagttccag gagttcggcc gagacatggc cgaggggctc gaggcgggaa
     6301 agatcctgga agaatcggga tacgacgcct tcaacgccga tgcgggaacc tacgaatcgt
     6361 ggtactgggc ccatcccccg gtgtacctgg aggaggggtg ctaccttccc tacacggagg
     6421 cgctgaaggg agtggtctcg gtgcccgtca tcgctgcggg caagctggga gaccccgacc
     6481 gggcggagcg agccctccgg gaggggcggg ccgacggcgt atccctcggc cgccctctgc
     6541 tggcggaccc cgcctggccc ctcaaggtcc ggcgccggga cgtcgcttcc atccgtccct
     6601 gcatcgggtg tcacgacgcc tgcatgaatc ggattgccaa gggaaagccc ctttcctgca
     6661 ccgtgaaccc cgccacgggg cgcgagcgcg aatttgccct gacccccgcc cgggagcggc
     6721 ggaggatcgc cgtggtgggc ggaggcatcg cgggcatgga ggcggcccgg gtggccgccc
     6781 tccgaggcca tgccgtggtg ctcttcgagg gcaccggaga gctggggggg cacgtcatcg
     6841 agggctccgt cccggagttt aagcgcgacg accgaaggct tttggcctgg taccgccggg
     6901 agctggagcg gggacacgtc gacgtgcggc tgcaccaccg gggagaccga gcggtcctgg
     6961 acgccgtgaa ccccgatgcg gtggtggtgg ccacgggatc cctccccctc gtgccccccc
     7021 tggggaaacg tcgggaagga gcccccgaag tcctcaccgc ccaggaggcc ctgcggggag
     7081 aacggcccct ggggcggcgc ctggtggtgc tggggggagg ctttgtgggg tgcgagctgg
     7141 cggtgtggtt cacccagcag gggcatgagg tggagctggt ggaggtccgg gaggggctca
     7201 tgacgacggg ggacgtgccc caccccaacc ggatcatgct cctggatctt ttgaccttcc
     7261 accgggtggg ggtgtggacg ggggcgcggc tctcgctcct ggcccccgag ggggcgacga
     7321 tccagagcgc cgacggggag cgtctccttc cctgcgacac gctggtgctg gccacgggat
     7381 accgctccga ccggggcatc tggcaagagg tctctggggc cttccccgag gcctatctgg
     7441 tgggagacgc cctgcgaccc gccaacgtgc agcacgcggt gtggaccgcc tacgaggtgg
     7501 cgcggcagct ctagggcaaa aaaacccgtc ggggcttcgg tagaccccgg atcctcgccg
     7561 ctctcggcat cgagagcggc gaggatccgg ggtctacaca cagagagcct cctgagggga
     7621 ttttggagag aagctatccg tcccggttga tgacggcgag catctccctc aggacccggt
     7681 cggcctcctc gtagggcacc tggcaggtgc ccacccgggg gtggcccccg ccgccgtagg
     7741 ccagcatcag ggcccccacg ttggtgtggg aggtccgctg gaggatgctg tgccccacgg
     7801 aaaacacgca gaaggccttt tctctgccat cgaaaaccct gaccgagatg ttttgctcgg
     7861 gaaaaagggc gtagaccatg tggcgatttc ccacgaaggt ctcggggatg ccccgcaggt
     7921 ccgtgacgat ggcattgccc tgggtccagg agtgggccag gatcatctcc ctgaagaggg
     7981 cctcttggtt gaagtagcga cgcacccgct cctgcacgtc gggaagacct aagatctcct
     8041 cgatggagtg cgtccggaga aggtccgcca ggtcgtccat gagctggtag ttggatatgc
     8101 ggtagtcccg atagcgcccc agccccgtgc gggggtccat gacgaaggat agaagcaccc
     8161 atcccgaggg gtttaagatc tcatcccggg tgaactgagc agagtcgcat ttgtccgcca
     8221 tggccaccac gtccttgagg tgccccaggg gcccctgatc tcccccgtag tactcgtaga
     8281 ccacccgggc gcagctgggg gcgggccacg cggcgcccct gaagctgtag gcattcatga
     8341 gcccccgctc ttcctcgctg gagtgatggt cgaaccacat gccgcacccc gggacgaagg
     8401 ggatgttgac gaggatgtcc ttctcattaa cctccaccag gccatcttgg atgtctttcg
     8461 gatggacaaa ctttctttcg tcgaagaggt ctagctcttt caggagaacg gcgcacatga
     8521 ggccatcaaa gtccgatcgg gtcaagagtc tcattccgtc acccccacat aggatcgccg
     8581 ctcattatac ctcatcttgg gcccccatct ccctgcgacg ggccaagctt gcccctatcg
     8641 ccggagaaaa aaatcgaaaa agctcccccc tttccgccgc cagcagaggg cggcgggagg
     8701 gttcgtccgg ctcccgaagg acttccgacc aagcctcgct catcccgcgc cgatcatcgc
     8761 cttcggtgag ggtctccccc tggggagcga tgggccacga tctggttaaa ctttcccacc
     8821 acgtccttcc cctcctcccg gtatcggccc ctttccagct gggccaccag ctccgctccc
     8881 acctccacca ggtcgtagtg cgtcagggat cggcggcgca ggatctcgaa ataggtctcg
     8941 tcctggaaca ggtaggcctc ggggaaaacg tcgagaaggc ggttgatcag gaggctcaag
     9001 gtctccgtgc acatgcgctc ttcgctcatc ttccacacca ggagggattt gtcgacggcc
     9061 atgatgggct taaacactgc caccacgttc tcctggggaa agggccccag ggcgtcgtag
     9121 atccgcccgt cccttacctg ggatatcata aatcccataa aaaactccac catgcgaagg
     9181 ggggcaaaga aatccttggc ctggatctcc tcttttagcc acttatagat caccacatcg
     9241 cccttgcaaa gagctccgcg ctgcatgaga tccttgatgt gcatcaacgc actccacctc
     9301 ctcgcgacgg gcagatctct cgccttttgg agcgttttct tccccctgcc gggcggcgcc
     9361 aacgggaggg tctccggtgc ccatctcttg gattttctag tataagcgcc cctcctcaaa
     9421 aaaggaatcc cgtcgggaag cctttaattc gcgaaaggga aatccgcgcc acgcccctcg
     9481 gggggcgcgg agagctctcc atgcggcctt tgccctccgg ggcgggctcc gttcgtccca
     9541 ggggccaccc cggaggccgg gggagatctc ggagccaagg ggacgatcta ggagggaagc
     9601 gacggaagac ttaggaaccc cagcgcaggg cagaggggtc gaaggagagc cacacttctc
     9661 gtcccgggtg gattccctgg tcgcgcacgg cgtctgcggt catccgaacc gcgagggaaa
     9721 gagaccccac ccccacgcgc accagcaccc ggtgaggacc cctttcccag aacatctgcc
     9781 ccacggttcc cttcaagacg ttgcgggggg cgctccctcc ggggggaagg atagataggg
     9841 caatggcttc tggggatagg agccccatcg tggctccgag gggcataggg ggggccatca
     9901 ccccctgacc gtcctccagc accaggtgcc ccggctcccc ttctttccgt tcccaccggc
     9961 ccgccagcac gttttccgcc ccggaggcga cgacgcgccc cccccggagc cccaccgtgc
    10021 ggctggccac ctcgtagagc cacaagaggt cgtggctcac caccaccagg gtggtgccgt
    10081 cctggcgcac cgccgacagg atggcgttcc gcacggcgcc agcgctagat gcatccacgc
    10141 tggccgtggg ttcgtccaga aggagcaccg ccgggcggag ggccaggcgg ctcgccagcg
    10201 ccacccgctg ggcctctcct ccggagaggg cgtgccaggg gcgtcgggcg aaggcgtcgt
    10261 aatccagccc cacggcttcc agggcctttc tcacccgccc gacggggtct ttttcccccc
    10321 ggaggcgcac cccgtatgcc acgttctcga agatcgatgt cttgagaagg tagggctcct
    10381 gcaggagcaa ggtggcctgg cgccggagct ccggcgtggc ggggcacgca ggagcccccc
    10441 gaaagaggag catcccttcg tcgggggggt ctaaaaaggc cagggtccgc aagagggtgc
    10501 tcttgccgct accgttgggc cccactaggc ccacgatctc ccccgggtgg atggcgagct
    10561 cggggacgtc cagggccact cggtctccgt agcgccgcac cactccctgc agttcgtaga
    10621 ggggggtcat atcgtcgccc tctgacgcgc caaggatagg acagagttca cggcgaaggc
    10681 caggatcaag agcaccagtc ccagggcgat gcccagggca aactccccct tgccggtctc
    10741 cagggaaatc gccgtggtga tggtccgggt gtgccacttg atgttgcccc cgagcatcat
    10801 ggagaccccc acctcggaga tgacccggcc gtaggcgttg gccgccgcca agaggacggc
    10861 gtaacgggcc tcgtagaggg tggaaagggc caggcgcttt ccgtcggccc ccagggtgag
    10921 gagggtcagg cggaggcgtt cgtccatgct ctccaccgca ttggccgtca gggccaccac
    10981 gacgggcagg gccaggatga tctgccccac cgccatgccg gagaccgtga aaaggagccc
    11041 cagctctccc aggggacccc tccgggagat gaaggcgtaa accaccagcc ccaccgccac
    11101 tgtggggagg gccaacaggg tgttcaccac cgtgcgcacg ccctttttcc cggggaaatc
    11161 cgcgtacccc aggatgaatc ccaagggagt ccccagggcg aggcacacgg cgatggagat
    11221 ggaagacatc ttcaccgtgg tgtagatcgc ggaccaggtc tcttcgtccc ccgtccacag
    11281 caggacgaag gcctggatca ctccctgggc gatgtagtcc atggggaaac ctccttcaca
    11341 caaaacgccg gggggtgagc ctttcgcacc ccccggatct catgtcgccc tcagacgccg
    11401 ccggagggat ctactcgtcg gcgttgggga aaaagagctg cttaccttcg agcttgaagt
    11461 ccgcgatgag cttctgggtc tcgggggaaa ccatccagtc ggcgtacttc agcgccaggt
    11521 cgtactggac gttggcacac ttggcggggt tgacggcgat gacgctgtac tggttgcgca
    11581 gggccgaatc tccctccacg aggatcacca cagggggatt gcccttggca ttggcctcgt
    11641 atttaataaa cgtgccccgg tccgccaggg tgtagccatt tttctccccg gccacgttga
    11701 tggtctggat catgccctgc cccgtggaga tgtaccatgc gtccttgtcc ggagcctcca
    11761 tccccgcgcc cttccaaaga accagttccg ccacgtgggt tcccgacttg tctccccggc
    11821 tcacaaaggg ttgtttcgcc gcagcgatgg ccttcagggc ctcacccacg gcgaggccct
    11881 tcaccttggc ggggtcggcg gcggggccca ggagcacgaa gtcgttgtac atcacttggc
    11941 gccggttgac cccgtgccct tcctgcacgt agatcttctc cgcgctgggg gcgtgcacca
    12001 ggagcacgtc cacatcgcag ttcttgccca gttccagggc cttgccggtg cccactgcca
    12061 cccactgcaa ctcaatcccc gtggcctggc ggaagaaggg ctccatgtag tccaaaagcc
    12121 ccgtgtcctc ggtgctggtg gtggtggcca tgcggagcac gggggcggca aacgccgccg
    12181 tggcgagcat cagaaccagc gtgacgaggg acaagatgcg cattgtacgt ttcattcttt
    12241 ttcatcctcc tcaaggacat gatctggacc ccagggtcca gggggttcct ctcgtcttcg
    12301 gtcttggatc cttgggccta ctgcgccgca gtgagggcgg cgatttccgc cggcagcttg
    12361 tcccgggggg tggcgggggc gggggtcatg aggccgaatc cccgcttctc caggatctgc
    12421 ttgccttcgg gggagaggag gaactggacg aactccagcg cccgggctcc gtttttcgcc
    12481 gacgtgggga tggtgaggcc gtagaccacg ggctcacccc gcatggtgat cttctcgtcc
    12541 cccttgccct tgatctccac cgacgccttg gcgtagtttt ccgcgaaggt gggatctccc
    12601 aggttgatct ccgccgggag gtcgatcatc tgcatgccgt ccgccacggc gtaggaacgg
    12661 tacttgagca tgtaccccag agaaccccac tccatgagct tcttgactcc cttggcgttg
    12721 atcccgtcca ccacccggtg ccccggagct tccatgaggc ttttttccaa ccccggctgg
    12781 ccgtagaacg cctcggcgag ctgcaacacc atgatcgccc ggtatccccc ggggtctttg
    12841 ttcggatcgg tgtgccccca cgccacgtcg ggccgggcca gcacccggaa ccagttcgcc
    12901 gccgaaattt cccccgcccg gggagccttg gggctgtagg caatgaccat ggcgttggtg
    12961 cagaagagga ggttccacgt ggcgtgctgg ggcttcagca gatcttcaat gttccggtag
    13021 tccgccgtga ggtagaggtc gcaccccccc ttgtccacga tggcctgggc gagctccacg
    13081 ctccccgccc ccttgtcctg gatctccaga tcgggatatt tctccagaaa gcgggcctcc
    13141 acatccttca gcgccgcatt caggcttccc gcatgccaca ccgtcaacgt ttccttggct
    13201 ccggagaccc ccgccgcccc cagaatcacc gccgccgcca acaacactcc catcagccgt
    13261 ttccccttca aaaaaaacca ctcccttcaa catgttccgg agcacactcc ggcttttgtg
    13321 acgccgtctc ctcacttccg acggatcacc gttcccaccg cttccccgtc gagtgcccgg
    13381 gtgaggtttc cctcttccag ggcgttgacg acgagaatgc ggtccagcac ctcgctccgt
    13441 tccaggatct ccaggcaggg acgctccagc accagatcgt ccagatcgag ctcgcgcaac
    13501 tccgccacag agatctcggg aataaactcc gccccggggt ttttcttggg atcgtcggtg
    13561 tagagccccc gttcgtcctt caaaaagatg cagctcttgg cgccgatgag ctccgccaga
    13621 aggagcgtcc ccacgtcggt acggtgcaac ggaatacgcc ccttctccgg cggcagggcg
    13681 aagtagtcgt aggggggcat gccgtgcatc acgggaatgc acccctggcc aaaataggtg
    13741 gcgagcttca cgatttcgtc gtggccgatc ttgagtcccc cccagggggc caggagagtg
    13801 gccacgagca gggcgttctg ctccgacacc gagctcccga acttggcgat gatccctgtg
    13861 ggcatcccca gctccaggcc gatggcgtag atgtggcgac tccgggtgcc ccctccggtg
    13921 gtgaagagca tcttgtgccg ggccttgttt tccacgatct cccgggccaa cgccggaagg
    13981 gccttggctc cccggtcaca gagggactgc ccgccgatct tcagcacgtg cacatcgggg
    14041 aagaggcgca cctggggcgc cgtttccaac tgttcggcga aactccggct caccaggctc
    14101 tctcccatga gccgactctt cacatgcaac cgttttccgt ctcgctcgcg ctccagcgcc
    14161 atggtcatcc ctccctcttc cggtcgttcc tttggattcg ttccttgccg ggcaccgtcc
    14221 gaaaggttcc gtcctcttcc cggtgcacca aaagcaccgg agatcccccc aggagaaagg
    14281 ggcgaaacca cccgcctccc cgaatcaacg ccccgggagg cacggaaaag gcgtcccgct
    14341 ccagccacac cacccgcagg ccccggcgct ccagctcctc cacgggaaca gcctcgggaa
    14401 gctcccgcag atccccccag gaaatgcacc tgccgcgcca ccccgccgcc gtgagcccca
    14461 cggcggcggc ggcgccgatg atgccgtccc ccgtacctcc gtgggcggaa aggtgcacct
    14521 cccgcgccgc ctcccgggca tcgtcctggg agagcacttc ccgaagagcc cgctccccga
    14581 aatctcccag gagtttcagc gcggggtgcc cctccggcgc cacgcagagc ccggggtcgc
    14641 tccccgggag ggcaaaagcc cccagatggg ccgcccccag ggtcgccagg agctcccccg
    14701 gtttttcttt ttccccttct ccctccaaca ccacgcacgc ggcgctgttg tgcgacgtgt
    14761 agggaatccg gggatccacc aaaagctgct ggcgcaccac gcccagacag cgccatccct
    14821 cgggaagagc tccgccgaag aaccgggcta ccttccccgt gccttgggga gcccccacca
    14881 catccgtatc gtccatgccc acaaaccaac gcattgattt ctctcctcgc tcggtggccc
    14941 ccaaaaaaaa ccgtcttcca aagaatttat ggaagacggc gagggacgca cgcatcagta
    15001 cgctccggcg accgcccttt ccattccggg aggctttccc gtttccagga aaacccatcg
    15061 gttcgccgcc atggagattc ctctgtaggc caacgaactc ggaggtcgta ttcatagatt
    15121 ttcaacaatt ctccgccctt ttttgcgacg cttcgtaagg ggggaggatc tttttttgct
    15181 ccgttttgat tttacgcaag gaaccgtcgt tccgtcaagc aaatgaagga agtctttgtt
    15241 attgttttct aggaacaggg agttcctccc cggtgcccgc cacatccccg gcgccctccg
    15301 tgccccccct cgtgtccctc acctgcgggg agggccacca gggtggctcc caccgcctgg
    15361 gccatggatt ccaactcctg gaggcgcaga tgggtggcga aaaaatggat ctccgccccc
    15421 gccacggcca ccacgcgaaa acgcggttgc cgatcctgct ctcccacttt gcgccgttcc
    15481 cggtagaaaa tcgttcccgc catgggcctt ccctccccac gaaacgcgcg tcccctcgga
    15541 ggtcattata ccgcaggaca tctccttcca gcccttttgc cagcccctcc ttccgcaaat
    15601 cccggggcgc atcaggagct tttctccctt tgttcgcttg tgttcttccc ccaaagtttt
    15661 atactcggat ttcggaagtt ccaccggcgc cgccggcgga gaactcttca gggagaaaag
    15721 gggtggcatc atgcgacaac gacttttggt ttgtcttgcg gtcctgggca tcctgggagg
    15781 cgcggtttcc gcatcggcgg aggggctctt ccggggagat acggccaaca cggggatttt
    15841 ccccgtttcc cgcctgcccc agggagtgct ggcctggcaa attcccacca gcgcccgcat
    15901 cgcgggatct cccgtggtgg cgggggggac ggtgtttttt gccaacgccg caggggagct
    15961 ggtggcgtcg gatcttcgca cgggacaggg gcggtgggcc ctgcgtcttg atggctccgt
    16021 cctcggatct cccgccgtgg cggggcagac cctcttcgtg gggacctacg aaggtacgat
    16081 cttcgccttc tccacctccc ggggcaccac cctgtggcac gcctccacca agggggcgat
    16141 cttcggcgct ccggcggtgg tggggtccac ggtctacgtg gggaacgccc tgggacagct
    16201 ccgggccttc gacgccgctt cggggcggga gcgatgggtc ttcaccggca acggctggtt
    16261 cgcgggaact cccgctgcgg gagaagggct tctcttcgtg ggggaccacg gaggacgctt
    16321 ccgggccatt gatcccgccc ggggaacgga ggtctgggga gccaacgccg ggggttccgt
    16381 ggatccctcg ccggtggtct ccggagggtg ggtgttctac ggcagcgacg acgggcgcat
    16441 cgccgccgtg cagatcaaga aggtgatccc caaatggcag gtcaaactgg gggcggggat
    16501 cgcctcatcc ctgggggtgg ccggagggct ggtgttcgcc ggatgccacg acggccaggt
    16561 ctacgccctg gacgcccgca cgggggccgt gcgctggacg tttctcaccg aagggccggt
    16621 gctttcctct ccggcctacg cccggggcac gctctacttc ggcagttcgg gcggcaccct
    16681 ctacgccgtg gacgccgctt cggggcggga gctctggcgc tttgccgccg gaagcgagat
    16741 ttcgggggct ccggtggtgg cggagggcat ggtgttgttc ggcaccgccg acggcacgct
    16801 cttcgcggtg cgctaacccc tcggcttctt cagttttcgg gcccccggtc ggcaggcgcc
    16861 gccgggggcc ttgccgtgcc cgccatcctc gggagggtgg cgcgatgacg aggttttggt
    16921 atgatggttc cgggaggagt tcgcccggcg cgagctccga ttcaaggtaa ggaggatccg
    16981 acatgcgttt tgtcatggca atgatggcac tttggttgtt cctggggtcc gtttcggcgg
    17041 gagaaggggc ccttcccctt cctcccgtgc ctgcggattc ctacgacgtg gtggtggtgg
    17101 gggcggggac ggggggatgt gccgccgcca tttccgccgc ccgcctgggc atgcgggtgg
    17161 cgttggtgga ggaatcgacc tggatcggag ggcagctcac cgccagcgcc gtgtccacca
    17221 tggacgacgt gcggcggaac cgcagcggcc tctacggcga ggtgatctcc cgcatcgtgg
    17281 cccactacaa ggccctgggg cgcacggtga acacctgtta ctggggatcg gacaccatcg
    17341 cctgtgagcc cgccgtgggg cagaaaatcc tctccgacat gatcgccgag gcccagggcg
    17401 gcggccccga ggggacgggg cgggtggagc tctttccgga aacccgggta acctccgccc
    17461 acatggaggg gtttcgcctc gtggcggtga agggggagcg gaatttccgc cccctggtct
    17521 tccggggcaa ggtcttcatc gacgccacgg agtgcggcga cctcatcccc ctcacggggg
    17581 cccggtaccg ggtggggaat tgcgtcgctc cccacatcga ccccgaagcc aacgtccagg
    17641 acatcaccta cgtggcggtg atccggcgcc tctcggggga tctgcctccg gagctccgca
    17701 tcgccacccc ccctccgggg tacgacgacc acgtggagga cttccgcatc ctggtcacca
    17761 aagacggaag cccgaaaccc ggcaccaaac ccgtgaaccc cgtggtgcac aacgcctacc
    17821 gggggcttcc ggatcccgaa aaccccctgt cggtggactg cggccgggcc aacacctggc
    17881 ccaacatctc caagacgggg atcaactggg ccaacgacgt gccgggggaa tcgggggatc
    17941 agccggggct gtcggtgcgc tacattgagg atcggctttt ccgcaaggcc gtggaccggc
    18001 aggcgctgct ccggacgctt cagttcctgt actatttcca gaccgaagcg ggggcctcga
    18061 actggaccgt ggacaccacc cagggtttcg gctcgttctt ctcctgcaac tgggaagtgt
    18121 ggaaggacat gcctccggag tatgcctccg tcctgcgcca ctttcccccc accccctacg
    18181 tccgggagag ccgccgcatc gtggggctcc gcaccctcac cgccgccgac gtggaacgga
    18241 acgagaagac ggggatggcc ttccggaatt ttccctcctc cgtggccctg ggggagtacc
    18301 ctgtggacat ccacggctcc catctggacc gctatctgga acacgatctg ggagagttcg
    18361 agcacacctt tcccaaaaag tggcgtccca gcaagggggt ctttcaggtg cccctggagg
    18421 tgctcatccc cgaacaggta gacgggctgg tggcggcgga gaagaacatc tccgtttccc
    18481 gcatggtgaa cggcgccacc cggcttcagc ccatcaccct cctcacggga caggcggcgg
    18541 gcaccctcgc cgcagtggcg gtgcagcggg gagtggaacc ccgccgggtg aagcccctct
    18601 gggtgcagaa cgccctgtgg cagcggcgga gccgtctcgc catggagatc tggcaggacg
    18661 tgcccataca gagcgactgg tggcccgccg tgcaggccgc ttcgctctac gacaccatgc
    18721 cctctctggg aaagaagatc ttcggcgcgg actttcccat gaaacggcgc cacgccatgg
    18781 agctcctccg ccgggccttt ccggggcact tcggcatgga cacctccgcc ccgtccatgg
    18841 agttcttcat cacccgggga gagttcgaga ttttgctgcg catgggcctt tcggcggaaa
    18901 tgccgttacc ccccaccgcc gtctatcagg acgacctgcg gggagaaatc acccgggggg
    18961 aggccctggc ccgcaccctg gaagtgctcc tggcccaaca ctgacatcgg cggagaccat
    19021 cccggggatt tcccggggca cacccacacg gaggcaggga tgatgatgaa aaaatccggc
    19081 atctttttcc gttttttccc cgtcctggca ccccaagccc cccttcgggg gagaaggggg
    19141 ctgggatcat gatcccccgg agtctggtgg agcgcatgtt ttccgccgag agcatcgaac
    19201 gttggaacga ccacccccgg cccctgcact tcaccgaaat ggccaagcag gcccacaagt
    19261 tcatgctggc gtggctcatc gcccggaacg aggaggagaa cggaaaaagc gtggactggc
    19321 tcgccctggt ggagggggtt ctgtttgaat tcctccaccg ggtggtgctc acggacatca
    19381 agcccccggt ctttcatcgc atgatggccc agcacggaga gaagatcaac gcctgggtcg
    19441 cccaggagct gaaggacgac cttgcccctc tggcgggggg cttctacggg cgcttccagg
    19501 ggtatttttc ctccaccgcg ccggagaccc ccgtcgccct ggagcgggac attctccggg
    19561 cggcccacta tctcgccacg cgctgggagt tcggcctcat ccgggcctgg ggggcccccc
    19621 tctacggcat cgacgagacg gggcgggaga tcgacgcgga aatcgagcga taccgccacc
    19681 tccgcgccgt ggacctcctc atggtggcct ccgccaaggg agatcggctc ggactccccg
    19741 ccttcatcga cctggtggga cagctccgct tccagaagcg ctggacccag accccccgca
    19801 ttccggagac atcggtgctg gggcacctct tcttcgtggc ggccctcacg tacttcgtgc
    19861 tgggagagac cggcctgccc cggaacgtgg tggtgggcac cgtgctcacg gggctcttcc
    19921 acgacctccc cgaggtgctc acccgggaca tcatctcccc catcaaaagg gcggtggaag
    19981 ggctggaaga cttcatcaaa gactacgagc aggacgtgat ggaagagaag atttaccgtc
    20041 gcatggcgct gcccgaaggg tgcgtccggg acctgcgcct tttcacccgg aacgagtttc
    20101 aggaccgcct gcggcacccc gacgaccgcg tcacccccta cgccccggag aacgcggaca
    20161 gcggttggcc cgtgctggga tcggtggtgg aggcctgcga caagttcgcc gccttcgtgg
    20221 aggcgtccct ttccatccgc tacggcgtca gcaccgccca gctccagacg gcgaagcagc
    20281 gcctggcgga aaagtggtcc cggtgcgtca tccacggcta cgaactgcgc cctctgtacg
    20341 actatttctc ctaaaggtcc cgagaacgca ccgcgaaagg agttggtttt tgtgaaaaga
    20401 gcgcttctgg tcatcgacgt gcagaacgaa tacttcgacg gtgccctccc cgtcacctac
    20461 ccccacggct ctttcgacca cattctccgg gctctggggg ccgcccggga ggcccagatc
    20521 cccgtggtgg tgatccagca ctccgccacc tcccccgaat cccggacctt ccgtccgggc
    20581 acaccgggat gggagctcca cccctccgtg gcggcccatc cccgggatct gctcctggag
    20641 aaaacgctcc ccggcagttt cacgaacacc gaactggaaa gctggctccg ggaacggcag
    20701 atttccaccg tcaccatcgc ggggtacatg acccagatgt gctgcgacac cacctcccgc
    20761 caggcgtttc accggggatt ccacgtggag ttcctttccg acgccacggg caccctggcc
    20821 tttcagacca ccgtcggagc cgtcaccgca gaggagttgc accgggccat tctggtcacc
    20881 caggccctgc gcttcgccga agtgctctcc accgacgcat ggatcgcccg gctccagagc
    20941 ggcgactgac ccttacctca gaaaacaccc ccggcagaag tgcggaagga agtgcccgcc
    21001 ggaaggtttc cccccgggcc acgctggtga tgcggggtta aatctgtagg ggcgaaaaat
    21061 atgctcgccc ctacattact ctccaggcac gaggcaaccc catgcgcccc tacaacaccg
    21121 cgagcgctcc agggggagag acccccggct tccagacggt cccccgggcg gcgttcattt
    21181 ccacccctgc cgccgtattt tttcgtccac ttcgcgacgg attctcgcgg catcctctgc
    21241 cgacaccgac acgacgatcc tcttcgctct ttcagcggca tcggcgtccc cgagtacacc
    21301 ggcggtgtag taccaccggt acgcttcttt gtcattttgc ctcacgcccc gaccccactg
    21361 gtacatctcg ccgaggttgc cctgggctaa ggcatacccc tgctccgccg ccttgcggat
    21421 ccaatccacc gccttcgtgt cgtcctgcgg caccccccgg ccttcccggt acatactgcc
    21481 gaggctgcgc tgggctgggg catacccctg ctccgccgcc ttgcggtacc aatccaccgc
    21541 cttcgtgaag tcctccgaca cgttcaggcc aaactcgtac atcgcgccga gacgccactg
    21601 aacgaaggca ttcccctgct ccgtcgcctt gcggaaccat tccatcgcct tcatgtcgtc
    21661 ctgcgacacg ccctggccgt tcgcgtacat cacgccgagc tcgaactggg cgtcagcatt
    21721 cccctgctcc gccgccttgc ggtaccactc taccgccttc gcgtcgtcct gcggcacgcc
    21781 gattccaccc ctgaacaatt ctccaagaac gtcttgcgcg gcaacgttgc cttcttccgc
    21841 catccggaca accgcatcct tcgccgctcc ggcgaggctt tgaccccggg cttcgtcttt
    21901 cggaaggccc gcgccttcga agtactgcag gccgagatag gcctgtgcca gagggtgccc
    21961 ctggtcggcg gccttgccga accatttcgc cgcctcgggt tcgttcacgg ggaccccgtc
    22021 accggaccag tacgcgagcc ccatctcaaa ctgcgcttcg accttgccgc cctccgccgc
    22081 cttttgcagc tcccccatgt cccgttgctg gcctgccaaa gatgttgtcc cgcatgtcat
    22141 caaaaacacc gccacaagaa atcccgttgc ccaacgcatt gttcgacctc ctctcaaagt
    22201 gtgtaccaga ataaccattt taacatttct cttcatcgca ccacgacctt acgaaaagga
    22261 tgattccggt ggagaggtgg cgccgccccc gtgtggggcc ctcgtccgca tctccggcaa
    22321 gggaaacgga agctcccgcc acggtgcgga gacgttgagg gggtacggag cgcacaagaa
    22381 ccccctggcc ggaaacggga catctgccga ggcaacgaat cttgacgaac tctggacacg
    22441 gcaagtatgc cagaacatga gaattggaaa attccttccg ccgctcttct cccgggccgc
    22501 acacggaaga ggccccggag cggacgactt cgccaagatc ggggcgacgg agggcagagg
    22561 tgcggaagga agtgctccgc cggagggatc cccctcgggc cacgctggtg atgcggggta
    22621 aaaagagagc cggctggaat gtgagcgctc ccggctcgca cgcccgagga atttcccggc
    22681 ggggcgaaag cctagaagtt gatgtacacc tcaaaaggct gaagagagcc ggctggaagc
    22741 cggcgctccc ggctcgcacg cccggcgggg taaaaggtga cgcggggcaa aaggcgtagg
    22801 ggcgaaaaat atgctcgccc ctacaacgct ctccagccac cgccttcgtg tcgtcctccg
    22861 gtacgcccca accttcctca caagatcccc gggggaatgc ccccggggat cttgtgctct
    22921 tgggaaaaat aagctcaacg gaactggaag gactggatga agggaaggat ccacccctgg
    22981 gattcctgat agcgggcggc gggcaccgag acgatgacca acgcccccgt gccggggaac
    23041 cggacgaaga gcgtggcgta ggccaccttg gtttccgcgt tcaccgacga gtagagcgtc
    23101 accagggctt ccttgccccg ggcggcgatg cgggattctc cgtggggggt cttccccgac
    23161 gccacctggg cttggaggtt ggccacgggc tcttccccct cgaaggtgaa gaaggaaatc
    23221 tgtcctccgg cgggcagaga atagatggcg ccaaaaagca gggattcctg aaacgccgct
    23281 ccggggggca ggggcaccgt atagagtccc ttggcgtcgg tgtacacccc cgccggagcc
    23341 ggtcccgtgg tggtgccgac gctgcccccg gtgcccgctc ctccctcgtc cgcaaagctc
    23401 accgaggtca tgatgccgtc aaaggccgac gccagggcgt cgaagtcctg ggcgccgcag
    23461 tcgaagagga agccaaaggc cctccccctc tccaccaggg caaaaaaccg cccccggagc
    23521 cgattcgtcc cccccgccgg agaaaagcga aagtccaggc ggcgcccctc tcttcccgcc
    23581 aggggcaccg cagcggtgcc cagcgattcg aaggcggcga aggcggtggc ggcgtaggcc
    23641 tgcacccgct ggaagtagag gtccacgttc ccatcggtga aggcttcttc aaacaccagg
    23701 agctccgccc gcacttggga accctcggga agcaggaacg atgccttcac ggaaggatcc
    23761 gggggatttc ccggcaccca gggcgggggg acgttgaagg tgaggcgggg cgttcccccc
    23821 gagctccctc cggcgaggcc cgccacccct gtgccagcgg tgctccccga gaccgtgccc
    23881 tggccggaga ccccggtccc tccctgtccc gcaacgcccc ccgagaccgg gccctggccg
    23941 gaggccccgg tccccccctg ccatgcggtg ccccccacca ggcgcacccg ggacaggagt
    24001 tcgtcgctcc ggagcttcac ctgggcgaag agctccgggg tggtgagcac gccgaactga
    24061 aaccaggcct tttccagggg cacgtaggag acctcccgct ggtaggtgcc ggactgtcgc
    24121 cgctccacca ccagatgcac caccgagggg atgccggaca ccaggccttc ggcgttcttc
    24181 aacagcgtcg tgttgtcatc gtaccgcatt tgctcccgga ggtacttgcc aaagccggcg
    24241 gcctcttcga cggtgtcgaa gtcgagccgc ttcacataga acactgcggc ggtcttgccg
    24301 ggctccaggg tgagatcgca ccgcaccatg tcccccgcgt cggggctccc catggtccac
    24361 cccggcgtca gatcgatggt gagatccgac gctccggcgc cccccgcccc caggagcacc
    24421 cccagcacca ggagcacgaa aagacccctc cgtctttttg gggcgcatcg ggccccgatc
    24481 ctccggagcc cgagggctcc cccgcttcgg actgcttccc caagggtgcg cactagaaaa
    24541 acctcctctc gaatgaccgc tctcccccgc cccgatgggg cctccgccgg agcgaggatc
    24601 ctttcgcttt gcctctcatg gtgctccctt cgttctccgg ccgaaagccc cgggggcttt
    24661 cacgccgacg aaggccggtt ttttcgcggc cggagggcgg gtgcttgcct cgccccccct
    24721 cgcctccggc tcctactgct ctcttcccaa aaggatgaac ggggcccaga acatgggatg
    24781 attcgtccgg gacaacaccg ccatctgggc ctcctggagg gcccgggact tcgacatccc
    24841 cccgagccac cgggagaaga acgccacgaa gagatcccgg gtggcctcgt ccgccacgct
    24901 ccagaggctg acgaagaggg aggacgcccc cgcgtggaaa aaggaccggg agaaccccac
    24961 gagcccctcc tggtgattca tgcttcccag ggcggtttca caggcactca gcaccacaca
    25021 gtccgccgaa agattcatgc cgaagacctc ccccgccgtg agtcttccgt cctgccctcc
    25081 tccgggggcg aggagaatca cgctgtttaa ggggcgtttc ggatcggcct tggcgtgggt
    25141 ggacagatgc agcacgtccg cttccggagc ccgggcgtag agggccgatt ccgtggcgtc
    25201 ccccccgatc aggagctggg agcggggaaa gagggccgcc acctgccggg cctcctcttc
    25261 cgcaaagggc agggggggca cggcggcgtc ggcgaaggcg gggttgccga atcccaccag
    25321 atttttgggc gtgggaccct cccttgccgc caacagggcg tagacgctca gggagggggc
    25381 gtaggagatg gcgaagcgcc gcaccaaaaa gccctcgccg tcccaaaagg cctggaaggg
    25441 cacgtaggag agcaccccct ggggcaccac caccacgcgc tccgccttcc ggaagggctc
    25501 ctccagggga gccaccagcc ccagatagag ccgggaaagc tccacgtagg gaatctcggc
    25561 cttgtagtct ttcagggcgg gggccaccgc ccgcaccgcc gacgacaccg catcccggga
    25621 aaggagcggg agctcccggc aggagaactc gttccgggag agaagaaaga ccaggacccg
    25681 atcggcaaga agatgataat ccgccaccaa cgtgccctcg gggatgctct tttgaatccg
    25741 gtccagcgac aggggacgca ccgccaccag ggagaggagc tccggcgaag ccagggagag
    25801 ctcgctccgc agaaaggcca gcttgtcccg cagggtccgc aggcggtctt ccagaacccg
    25861 gggcacgggg gcggaggccg ccaggaggtc gctctttttc cgggagagaa cctccacttc
    25921 tccctccagg gcggtgatcg cccccaccag atcccggtcc ttcggcagga gcttcaccgt
    25981 gcctcccccc accacgtcga gaaaggcccg ggccttcatg cgctccgccg cgtcgaaggc
    26041 ctcggcgaac cgccccgacc gcacgtacag atccatgagc agatcgtaga cctcgtagcg
    26101 ccgctccatg aacgccatgc ggtgctccac ggaggccagc tccgaccggg aacgttccag
    26161 cacccctgcg gcctcctcca ggaggggcaa cgcccggtgc cgatctcccg aggccaccag
    26221 ctcccgggca tattccaaca gggaaaagaa gatcaggggg cggtgctcca ccctccgggc
    26281 gatctccacg ctccggagaa gatcctccac ccccaaggcc cgacgtcccg cggcgcaccg
    26341 ggcgtagccc cggtttgccc gcaccagggc ttctcccacg agatcccccc gcgcttcgta
    26401 ctccctgagg gcctctccca gggctttgtc cgcgttgtcc caggccccca tctccacgtt
    26461 caagttcgcc acgttcacca gaaggtccgc cccctggcga tccatgccca ggcgctggca
    26521 gagctccagc gcccggcggt agtacccctc ggcctcggcc agatacccca gccgccgggc
    26581 cacgatgccc agatttccca gaatccccgg ctgggagcgc acatctcccc gctcctgggc
    26641 cagctccaga gcccggcgaa ggagcttgtc cgcctcggcc tcctgcccca gctcctgccg
    26701 gagggtggcc ccgttgatca gcacccccac gtcctcgggg acgtcgtgac ccatctgaaa
    26761 gtccagggcc ttctccagat actcgctggc cttgccgtac tccccccggg caagaaagaa
    26821 cgtccccaga ttgttccagg cccagagcct cccggagccc agatccagcg acaccgccag
    26881 cttctccgcc cgctggagat cccccagagc tccggcggag ttgaaaagcc cctccgccaa
    26941 aaactccgcc cggttcaacc gcaccacaaa ctccagccag ggggcccccc gggcctcgtc
    27001 gagggcccgg tcaaaccact gccgggcctc gtcgtggcgc cccagcagat accgcccccg
    27061 ggcgttgtgg ttcgccgcca gcccccggat ttcggggttg tccagatttt ttcccagaga
    27121 aaaagcccgc tcccagaagg ccaccgcccc gggcacattt ccccggtggt aggcccaaat
    27181 cccctcttcc agggccccca cactcgcgcc gtagtcgctg gacgcggccc gggccaacgc
    27241 ctgaaggcgg tccagccgct cccggcttcc gttccagtcc cccatgccct gggaagcccg
    27301 gatggccacc gccaaagccc gcaggagccc ctcccggtct tttccctgtt ccaggaactc
    27361 cgcagcccga tccgccgccc cccgggcctc ggcgtcccgc ccccggcgcg agagcacgta
    27421 gccccgcacc acgagctctt ctccccgctc ccgggcgcct ccggtggagg agggcaggag
    27481 ccggaaggcc caggcctccg cttccgcgtc gtgccagcgc ccctgataca ccaaggccat
    27541 gatccggcgg cggagctcct ctcggcgacg gtccacctcc tccggccccg gggcgcttcc
    27601 caaaagcccc cctccgtccg gagagagcgg gggggccccc ggcgtttctc ccgccgcccc
    27661 ccccgcgagc cagaggagca cggccatgag gcacagggtc ttcatgcccc gcctcctagt
    27721 ccttcttgga aggtttgctc accgaggcct cgtaggacgt gatcatccgg ttgaacacgc
    27781 tctgcaagcg ctggtcgaag acctcccggg gagcggtgca caccatggcg gtgcccaggg
    27841 cgaagggcat gccgccggac atctgggacg ccatgacata ggtctgcacc atggccatca
    27901 tggcccgccc ctggaacgtc cccgccagaa ggaatctccc gaaggtgagg gcgtagccgc
    27961 tcccctgaaa ggtggggtcc tcccggaact cctcgcggac caccgccaga tcggggacgt
    28021 tcgacgtcag aagggtcgcc acctgaatgg ccagatcgcg ggcggaagac acctgcaccg
    28081 tggcccccgg ggcgatggtg aagcacacca tccccgaggc gtcctcggcg aaggtcaccg
    28141 tgcggttgaa cgtgtccacc tgcaccatcc attttcccgg atattccagc cgccccaggg
    28201 gaaactggct gaagccgaag gccgtggaaa gatcaaaggt tttccacacc gccagatcgc
    28261 ccacgggggt ctgtcccaga gctcccagcc cccccgtgcc gcccaccaga ggcgcgggac
    28321 tcggcgccgc gaggagggga tttcccaaca gcccccccgt gccctgtcct cccacgaggg
    28381 gcgggttccc cgtcgctccc cccgtgtcct gcccccccac gaggggcggg agctgccctc
    28441 cgccggacgt cgccggagaa ggggccggag caaaggcccc ggaaagggag gggagggtcc
    28501 ccgtgccccc cgccgccccc gcgtcggggg tggtgggcag agagggcagg gtgccctcgg
    28561 cgccgccccg gagcgccccc ggcaacgccc ttgtgcccca ctcctccaga ggcgtgagga
    28621 gggaaggccc tccgcgccgc cgccaggcct ccgagggcac gccaaaaacg atcacgtcgc
    28681 cggtggcgag gcgcacctcc accccggaag gaacctccgg ggcccccgcg cccccccgga
    28741 gcgccggcgc ccccaggacg ttcccgccaa agatcagggg atgggccacg gaatccgccg
    28801 ccccggctcc tccggccagc agcgccaccg ccgccagcgc ccccaaggct ctccgcccgc
    28861 tttttcgggc tccatcgctc cagaaagaga ttttcacgat tgatccactc ccctccggcc
    28921 ccaccgtgcc tccatgtcaa accttgccca ccgagcccgg cccggaatgt ttcctcccgg
    28981 cccgggccac gacgcgcctt tccatccgct tttttccggg agccttcgcc tccggatgag
    29041 agccagtata cgccggagag cggagcgccg catcatcttt tctccctttt ttctggagaa
    29101 accgatgggg tgtttccgga agatggtttt ttttcgtttt acgggtaaaa tgtggaacac
    29161 acatcacccc gcccggagga aaaaaccatg ggaaatggct ccaaaccctt gcgcctgctg
    29221 cacacctcgg attggcacat cggacacgcc atctacggcc gaaaacggta cgctgaattc
    29281 ggcgcctttc tggactggct cggcgaaatc ctcgtccggg aggacatcga catccttctg
    29341 gtggcggggg acatcttcga ctccagcgtt cccagccacc gggcccagga gctctactac
    29401 cgctttctcc gccggatcac cccttcttcc tgccgccatg cggttctcgt ggggggaaat
    29461 cacgactccc cctccttcct cgacgccccc cgggagctcc tgcaatccct ttcggtgcac
    29521 gtgatgggag ctccgggagc gcgccgggag gacgaggtga aaatcctccg gaatccccag
    29581 ggccatcccg agctcctggt ctgcgccgtg ccctatctcc gggacgggga cctccgcacc
    29641 gccgaagcgg gagaaacccc cgaggacaag gaccgcaagc tcgtggaggg gctccgggcc
    29701 cactacgccc aggtcgccgc cctgggagag gagatccggc aacgcgaagc ccccggtgtt
    29761 cctctggtgg ccctggggca cctcttcgtg gcggggggca cctccgccga gggggatggc
    29821 gtgcgggagc tctacgtggg ctccctggcc cgggtttcgg aggacatttt cccccaaaat
    29881 ctggattacg tcgccctggg gcacctccac gccccccaaa gggtggccgg ggcggaaacc
    29941 cggcgctaca gcggcgcccc tctggctatg ggcttcggcg aggccaccca gcaaaaaagc
    30001 ctctgccggg tggagttctc cggccggagg gcctcggtgg atcttcttcc cgtgccggtt
    30061 ttccagcgca tggaacgcct gcggggatcg ctggaggaga tgctttcccg cctccggaac
    30121 ctcgccgccg aaggaaaatc cgtgtggctc gaagcggact acgacggcga agagatccag
    30181 ggagatctgc ggtcctccct ggaagaggcc gtctcgggca cggccctcga cctcctctgc
    30241 gtgaagaacc gccgccttcc ggcatgcgtc ctcttcggcg aggaggaaga cggagctctg
    30301 gcggacctga acgaaaacga ggtctttgaa cgctgtcttg aagaggcctc ggtggacgaa
    30361 agccagcggg aggagctccg ccgggcctac cgggagatcc tcctctccct ggcccaggac
    30421 gaccccctgg gagaagaaag gagcggcgcg tgcgcatcct ccacatccac ttcaaaaatc
    30481 tgaactccct gcagggagaa tggaccgtgg acctgaccca ccccgcctac gtctcggagg
    30541 ggatcttcgc catcaccggc cccacggggt cgggcaagtc caccctgctg gacgccctct
    30601 gcctggccct ctacggcgcc accccccgcc tgggacggat caccaaaacg acgaacggca
    30661 tcatgtcccg ccagaccgga gagtgcttcg ccgaagtgac ctttgaaacc ccccaggggt
    30721 gcttccgctg ccactggagc caacgcaccg cccggaagaa ggccggcgga gagctccagc
    30781 aggccaggca cgaagtcatc gaccacgcca cggggaacgt cctcgaagaa aagctctcgg
    30841 aggtgcctcg ccgggtggaa gagatcacgg ggctggattt ccggcgcttc acccgcacca
    30901 tgctcctcgc ccagggagat ttcgccgcct tcctccaggc ctcttccgac gagcggggcc
    30961 ccctgctgga acagatca
//
LOCUS       DAOOER010000022        30411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_1728_length_30411_cov_8.708295, whole genome shotgun sequence.
ACCESSION   DAOOER010000022 DAOOER010000000
VERSION     DAOOER010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 30411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30411
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1728_length_30411_cov_8.708295"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2061)
                     /locus_tag="PL162_04610"
     CDS             complement(<1..2061)
                     /locus_tag="PL162_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91335.1"
                     /translation="MKRRFGLGLVMALCLCVASGAWAVTVSTPVLPGDFGQGTTGTDV
                     TMGDVQNGYRVVLYSADRTGKQYIGFSGAQHAGEWNDFVYRSPDAAADYDNASDLDFV
                     FIRAGVDAKTHPFSADYNSAVVDTENEIWLTGFNGAAGWSLHSGDEFGPIGTVSYDLN
                     RQWPFTVEWNCPACGQNPCDTLQTAKLHINLMSRDGAGTFTTSADVESVDFAVYIPCD
                     TYEKRWQVMPQNLNCLTDGVVETPVHYLDMVLYGTSVAGSHALDLGPYVIDGTSHTER
                     FNTTHKLWGPTAASITGITESDRLMARGDWVAERILNKGTYLARALADNRFMWSAVKE
                     TSSDASGYQYRILGQAYASRPAFAYLMDQDKAATHAGWYSFLHEHSSWTTSQVRSLSP
                     GFALYYGRFGENFVDQNGTELNVLMDFDATAATNDFCQTTNLLAKRWGDANWCYEWDC
                     SATCVRIERRWVDDAMLVDLSNDLGVASKDMGVYWADADLTDICGEDDPCGSQEFFQQ
                     VADTTACAPAGTTLCRAVSLDMWADWSAACPITIYDGKTFFQPVKVTFELNQNHICDP
                     VAKADIIANVANIDGTALNKGPIDFFMEAGNAKRLTFGVYGYDGNWYNLGYLADAFFA
                     SADKTAKPWLGMKNFFEVWPESADRTKSVKAAWAAKDKLDFSFYAVLVAGTNPLPGGK
                     PVHAK"
     gene            complement(2302..2799)
                     /locus_tag="PL162_04615"
     CDS             complement(2302..2799)
                     /locus_tag="PL162_04615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HQF91336.1"
                     /translation="MAEGEDPMAPPEGLRGKRRKIRRRGFTLVEILVVTALLALLAAV
                     ILPRLSFGVSDPHTALQRALWAAVELASGGMPLRLRMAEGTLVAEVRTPRSPDAPPGA
                     EEDRWSPVPLAEPLPGRGWGGEGPCIVGSDGAVSPWSVTAPDGTTYVVAVTGRVYPQE
                     SSPKN"
     gene            complement(2954..3382)
                     /gene="gspG"
                     /locus_tag="PL162_04620"
     CDS             complement(2954..3382)
                     /gene="gspG"
                     /locus_tag="PL162_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HQF91337.1"
                     /translation="MKTLKRRRGFTLVEIMVVVIILGLLAALVVPRVLGQGEEARRTA
                     AQVQLKEIEQALDLFHLDNGFFPTTDQGIGALVSKPAGGPEPRKYRAGGYLKKMPADP
                     WGRPYLYRCPGEHGDYDILSTGPDGKEGGTGKDADITNWE"
     gene            complement(3480..4634)
                     /locus_tag="PL162_04625"
     CDS             complement(3480..4634)
                     /locus_tag="PL162_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HQF91338.1"
                     /translation="MPAYRYTAYDAAGKMLSGVVEASGEPGALSQLAERKLVVVDIAP
                     TETARKGLTLRALPLDVHYLFCKSLAGYLKSGLPVTDALRLLAKQSPDPRLALAYEKL
                     LEDVQGGRRLSTAMEQLGIFRESLVGMVASGEQSGSLPVILRRGADLFRQEHELRRKV
                     KSALTYPLVMLVFGLGVVVFLLTYVVPKFAGLFAELGQVLPLPTRILLAVADGVSWAG
                     LPLAGLGALAFFWLRRRKKPLNIPGSAALRERIALSLVFSQVATLVEAGIPLVQALEM
                     AAPLDRNPERWKAVADLVRQGHRFAAALEKEGPVTEDVVYMIRVGEMGNDLPEALRNA
                     AENAWEVAQARMERLANLAGPALILLLGSGVGFVVVAVLLPIFDISALVK"
     gene            complement(4806..6284)
                     /locus_tag="PL162_04630"
     CDS             complement(4806..6284)
                     /locus_tag="PL162_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HQF91339.1"
                     /translation="MTLPRPEDIRDLLPGEVSLDTLRATNLLPLRREEGVLVVGASSL
                     EAWPSAQLLAASLGCAAELEVHPPEEIASVLRKLYDLRSSGAEDAARSVEGVEDLSAL
                     SREDVLSDSVDAPVIRLVNGLILEALRERATDIHVEPYEDEVVVRYRIDGVLHERLRL
                     PKGHQAPLTSRLKVMARMDIAHRFAPQDGRIGITLGDRAVDIRVGAIPTQHGERMALR
                     LLDKSSGLLTLEQLGMAPREREIMGRIIETPHGMILLTGPTGSGKTTTLYAILQQVAQ
                     PAVNVITVEDPVEYDLPGVAQIQVNEKAGVTFASALRAILRQDPDVVMVGEMRDYETA
                     HIGVQASLTGHLVLSTLHTNDSVGAVARLVDMNIEPYLVAGCLVGVVAQRLVRRICPR
                     CREEHPAPPLLRRHGVDRVWQGRGCEHCRHTGYRGRVGLYEQFVSSEALADAIARGAP
                     GAELRELARAGGLVTLWEQGLERVKEGTTTPDELTRVAGQEA"
     gene            complement(6281..8317)
                     /gene="gspD"
                     /locus_tag="PL162_04635"
     CDS             complement(6281..8317)
                     /gene="gspD"
                     /locus_tag="PL162_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HQF91340.1"
                     /translation="MERHEKKATISQGHPGGFGAGRKGGAILAWALFLLFLPLAPLSP
                     LIPAPMEARAQEGVEPDEQELVKMAQAMRQAGQVQFNFKNLDLVKFIRFMSELLGENI
                     LVDPSAKGSITIVSTRPVSLRESRQVMLSALEMNGLALQSEGGFSKIRSQSTGPSTDN
                     MVRKGKAGPGYGEEYVVQVVPLEYVAAAYAQSAVSTALGGAVVFFPTETGNGAILSGK
                     ATLVQRAVSVLHALDVPDNLRDVHVVDLLYARPSVIAGYITQAGQSGASALTGVTAIG
                     DDTRQKLVFVADRTTAKEVKRLLIEMDVPAKSSGFYVYKLQNADATTVATQLTQVLTL
                     ANAALPERAGMIGTVVVPDVPTNSIILNVPQDQYVMMEQLIRQIDVQPKQILLRGLVA
                     EVTLNKLNQAGIDWATWGGSIGGDALVAGQVVMGESGVPSAFLEWYRSLMTHEEIKYD
                     DRGNSYKITEYDSKALLYSYVKLLNKYDAINVLSMPRLLCTDNKESTLQVGQVIPQLK
                     GAVSDTSNPSAVQNSYEYKETGIILKVTPHVRSGTLVALDIDQTLEEVLTTTANATPT
                     TAKRQVKTTVLVQDGQTIILGGLIKEAEKSMKNRVPGLSYIPIVGNLFTTTVKQKEKI
                     DLMIFLTPYILESPQEASEITRQYMVSGDVPGNPEEDLVQKRLEELYRKAVKRN"
     gene            complement(8397..8771)
                     /locus_tag="PL162_04640"
     CDS             complement(8397..8771)
                     /locus_tag="PL162_04640"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HQF91341.1"
                     /translation="MARTRSASPEVKRRNLRGAASGFTLVEIMVAVAILALVSVGALR
                     LAALSQRTLDEVQIQRRLLDEARALQVIQRAGTLPDSGSSGDLSWSLLKEEVEIPPTG
                     LRLPYKRFTVTLSGRTLEIWVP"
     gene            complement(8788..9747)
                     /gene="gspC"
                     /locus_tag="PL162_04645"
     CDS             complement(8788..9747)
                     /gene="gspC"
                     /locus_tag="PL162_04645"
                     /inference="COORDINATES: protein motif:HMM:NF041616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HQF91342.1"
                     /translation="MTLPSLKQILRPSAVGGGSHLLVALVRWGAWAVLALVAARGVAF
                     LGEEFLRRHTLALRTEHVLQSVLGSRESASRGAPPGVVAFVERNPFGVPPRPAVASVP
                     VAETTGPGLSLADVQVEGTVAGVGALVSTEEGPRWILQREEFRGYLVFRVQAGGVLLS
                     RDGQETFLPLVFVGGSPGAPASLTVAGSPSSGSSAPPALPPAFQGKVSSAAPGKEGVV
                     AREVVNALLMNPFDELKLVRLIPKVDDQGKAGGLEVAALEDKSILSTLGVRPGDVIKG
                     INGITMNNMGDVANAINSLMGGSKFDVAIVRDGKPEQLSYAVK"
     gene            complement(9728..10345)
                     /locus_tag="PL162_04650"
     CDS             complement(9728..10345)
                     /locus_tag="PL162_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91343.1"
                     /translation="MKRFKIIAPPLIFFIACFVGGLGLFFPLQEAVDWGVALLIREGD
                     RRGIEITVRNATAGGGWIPSLTLESIEGRSPLFSARSRGVELRWNPLPLLGGRGAFVL
                     SWEEGGVEAFGKEASWEGGQTQVELTPGTGRFLALELRGELQARGDVTLDLVASRISA
                     ADMRLKVPGELDGVLEAAAAFLPLQKESEGQWHLVREEGHDAPQS"
     gene            complement(10342..10869)
                     /locus_tag="PL162_04655"
     CDS             complement(10342..10869)
                     /locus_tag="PL162_04655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91344.1"
                     /translation="MMKGDDSFLTSLGGALGRFREALGANAEFRRFVLLLLGVLVAWS
                     VGFQLAQEASSEWQRSSLLEDRFRKFLDVVSQYRALGGASAPGVREGRASDPLEAFSG
                     VADRLGMKGRIKDLSASDQGVVVVLERLYAEEFMTLLQELDGAGLVVRSAELRTLPEK
                     DGPLFVLSLIVGVES"
     gene            complement(10866..12041)
                     /gene="gspL"
                     /locus_tag="PL162_04660"
     CDS             complement(10866..12041)
                     /gene="gspL"
                     /locus_tag="PL162_04660"
                     /inference="COORDINATES: protein motif:HMM:NF041617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HQF91345.1"
                     /translation="MGRRHLASWGVPGAGGYWILRQGSVVRHEGLPEDFSGVLLYPFR
                     TLSVHPFSYPFGNLSRIREALALQYRSLLLGGQGVEVLPTVWRREGKGASGVAWFWSR
                     EELAFLEGRRGPRLTVWPLPLALASQMEGDGAALWVDGGAVSSLAWRQGIPFLARWKT
                     LGPQGLEGEKAWFRQMGGEAFSFVEETLDPRTPQEALRRLGAAADATWKAFPLLHEVD
                     LSSGGVDALVAWDQALLSLRGPLGALLVGGLLFSGVQGGELWSALHRQEDLQARAAEA
                     YAQVFGGTPTDPLSQARARLAEARGENRQARGASLGDVIRFVGETLSEGGDKMTLETL
                     RYSASGAQMGGVLPDVGTLAKVKERLKKGELSSRVGDVQQTPDGQVRFTVEVGWQKP"
     gene            complement(12041..12964)
                     /locus_tag="PL162_04665"
     CDS             complement(12041..12964)
                     /locus_tag="PL162_04665"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HQF91346.1"
                     /translation="MWTGSRSRRPAFVLAAVLMISVVLIAATTGFAWFARNQLRRVEG
                     ERTALRSRNLAFLTAFFVARGLAADRSDYDAPDKPQFQPMAFPAGEGVVSLHLVPLDR
                     ALPVNALFLPDGTTLRQEMIHPWEELWNRLGHGELAPVVLDFLDADGTPRLGGGEREG
                     FINRPLGDLSELLLCPGLSPPLLKGPSGPSKKGLEDFLTVWCDGKINVNTASEDVLTL
                     LHEGFTPEGARALGAYRVDHPFKKIEDFFEAPAFPREALGSVMNLVGVKSSYFGLTVE
                     VTWAGKRSWVYDIVLKKDHQTVRAVAWEERH"
     gene            complement(12942..13565)
                     /locus_tag="PL162_04670"
     CDS             complement(12942..13565)
                     /locus_tag="PL162_04670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HQF91347.1"
                     /translation="MILSSPGGARRPPRGFTLVEILLVLALVGIVAAVGIAPVVHTVG
                     LLTRVEDDFAEDTAFRLAASRMAGDWQSLLPVPQGPAFRLIRRDKFGGAANDAVVAWS
                     GVSRGGDRPQGSVLYRVIWPDDPFSAMIPGLYRWFLGGLLPHQVDPDGVSLDRGQLVL
                     PDVTALRLRVHDGAAWVGQYEGEFPPGVEIHLERDGKALTYVDWLPQ"
     gene            complement(13562..15415)
                     /locus_tag="PL162_04675"
     CDS             complement(13562..15415)
                     /locus_tag="PL162_04675"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HQF91348.1"
                     /translation="MAKNKVLRKAPPGPMISPSPPALAPWWMTFPLLLVALAVPNLVF
                     SGLSWFDTLHLMKWVVTAVPVALCWVVAGSRVAWYGADRLNFRWDAFGLAWFFMLLYF
                     ALQPLWVPITSGPTLVKEWFFLATMLGVYLLALNTDPGRWRRDLRWILLGAALNGAIN
                     VGFAELQMRDLVTPDLFFILHTPGFYIGNTGYQNMMGLWMFVGAYGMCWCFLTGAFRR
                     EDGSHPFFWWVALGLLAVDVYGLAASTSRSAFLAFLVGLLTLGVAFWAQAEDRRRVLK
                     RVLIFTGAFFLAYGVAMMGVRGADVFFYKFGDMVVNYKGIGGRSAIWATSWTMIRQHP
                     EGVGLGHYKWHYLDAQREMFRDFPENEFQFTNWAHNEYLQWFAEGGIVGGAILSLMVL
                     WWLWRFCRALGRRQKLSPPAVWACGFVTSYFFAMVWDRPLHRIEDAVWLALALGLSAR
                     EILLPSEEDRGTLGRMPLVGRFLGGFAAVAALGGLCFLGDGMVGDKILARSLRASNAQ
                     EYKRLLEKAQDHYMVHLDAERQLAILLIRAGESSQDGDLLLEGLNRLTQYFYRESTAE
                     DLMTLLQWADRLHSREMIDRFTAYLQPGSYQVVPAPGASAPPPMSAESVEP"
     gene            complement(15591..16952)
                     /gene="dnaB"
                     /locus_tag="PL162_04680"
     CDS             complement(15591..16952)
                     /gene="dnaB"
                     /locus_tag="PL162_04680"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HQF91349.1"
                     /translation="MVESLFDRVQPHSLEAERAVLGACLLDRDALLSATEILDPEDFY
                     DLHHRDVFSLVGEMARKDLPVDPLTLWEEIGRHAGLAERLGGQGFLAAVVDSVPTTAN
                     VEWHARIVRNKALRRRLIAAGGLISRLGFAEDREMEDVLDEAERLVFDIAQRGNDRPF
                     RHIREIVPEAFQHIEKAFLRKDQTIGTPSGFVDFDHLGGGLQPGSLIVVAARPAMGKT
                     AFALNMAQHAAIKEKKPVLIFSLEMGGEQLVHRLLGSEAQINIHDLRTGNLSRDQWDR
                     LAHAAGRISAAPIYIDDSSDLSTLDLRARSRRFVALMKGQPVGLVVVDYLQLMRSLRS
                     AESRQQEVAEISRALKAVARELSVPVVAISQLSRAVESRTDRRPMLSDLRDSGAIEQD
                     ADMVLLLYRPGYYDRKDGDEEDPRAEVILAKHRNGPTGTINLIFMKEFAQFVSSERRP
                     YLP"
     gene            complement(16954..17400)
                     /gene="rplI"
                     /locus_tag="PL162_04685"
     CDS             complement(16954..17400)
                     /gene="rplI"
                     /locus_tag="PL162_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HQF91350.1"
                     /translation="MKVILKQDVAKLGKKGDLVEVSDGYARNFLFPRQLAEEGTAGKV
                     KDWENQEDTKRAKAEKAEQHAREVGRHLQGKRVVVKASAGDAGKLFGSVTSAQVAEAI
                     ATQLGAKVDKKDLRLPEALRSVGVYPVTVRLHAGVETSLTVSVEAE"
     gene            complement(17452..18405)
                     /locus_tag="PL162_04690"
     CDS             complement(17452..18405)
                     /locus_tag="PL162_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HQF91351.1"
                     /translation="MHDPRPLVESALLAALAAVLFLISSALPLVGVVAALICPTPLVV
                     LGLRHSLRHAILGTGVAGILTVIFSGPLGGLFFTLGFGVLGVGLGVLAKKYDHAEEIL
                     LYGVLVSLASKLLLMGVATAVTGINPFSVDPDQMRLTMQQAFDLVGGWGGQGAPAALE
                     PVRAQMEASFQIIPLIFPALLVMAGALDCWLSYAVSAAVLQRLGQRRLPPIPLFDTWR
                     FPRSLVWALLASLVFPLLDGGGEGRGILFRAGINLRMVVTMLFLLQGMAVLGAFLRRW
                     RWGKLRWVAFFLVMAFPFLSQGATVLGIADLWFDFRARGVE"
     gene            complement(18521..18787)
                     /gene="rpsR"
                     /locus_tag="PL162_04695"
     CDS             complement(18521..18787)
                     /gene="rpsR"
                     /locus_tag="PL162_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HQF91352.1"
                     /translation="MAAPSHGHGGPAGGPRKRGKRRPKVCFFCVDKVDHVDYKDVEKL
                     RKYISERGKILPRRVSGNCAKHQRRLTDAIKRARMMALVPFSAE"
     gene            complement(18841..19347)
                     /gene="ssb"
                     /locus_tag="PL162_04700"
     CDS             complement(18841..19347)
                     /gene="ssb"
                     /locus_tag="PL162_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HQF91353.1"
                     /translation="MRGFNKAIIMGNLARDPEVRYTASKQAVARITVAVNRRWKGSNG
                     EVQEQVDFIPVSVWGIQAEHCEQYLSKGSGVLVEGRISVRSYEKDGQKRFATEVVAQS
                     IQFIGGRRDEGGAAPSRGGPHRDEPATRPPRFGSLRDEKGFTEEDFPLDISDMPEDRG
                     GEEADIPF"
     gene            complement(19360..19644)
                     /gene="rpsF"
                     /locus_tag="PL162_04705"
     CDS             complement(19360..19644)
                     /gene="rpsF"
                     /locus_tag="PL162_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HQF91354.1"
                     /translation="MRPYEMMVLFHPDVEDHKVLAEEIGEVVKGVGGEVSKIDHWGKR
                     RLAYPVDKKQEGYYTLYQLTLDPGQVKELDRLLHIRTNVMRHMVVRKDEL"
     gene            complement(19725..20039)
                     /locus_tag="PL162_04710"
     CDS             complement(19725..20039)
                     /locus_tag="PL162_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HQF91355.1"
                     /translation="MPRLRWVFVLTVTGLCFAVMLTAYGFELARIARLSYLVDQRMDE
                     IVRLSRRMQTLREEIAYYETPEGVARLAREQFNLLYPDETVFRVVYTSGDEVRILSRD
                     GP"
     gene            complement(20053..21720)
                     /gene="argS"
                     /locus_tag="PL162_04715"
     CDS             complement(20053..21720)
                     /gene="argS"
                     /locus_tag="PL162_04715"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HQF91356.1"
                     /translation="MLKQEISKVLEDLARDRGVAPELLPEVYLERPKRPDQGDWATNV
                     ALQGAKVFGSHPREVAQALAERLTPGEKSCVAGVEIAGPGFINFRLSRGWMGAVAERV
                     FSEGERYGRSDLGRGRRVQVEFVSANPTGPLHVGHGRGAVVGDVMTSILEFTGWQVAR
                     EYYINDAGLQVDNLGRSVQSRYFELLGQGERAPFPEDGYHGDYIRDVAQGVLDQEGPH
                     LLDLPLEETLPFFREYGCRTLLEVIRKDLEFFGVRFDVWFSEKSLYDNASVPGMIDHL
                     RGRGFVYDQDGAVWFRATAFGDEKDRVLIRSNGAPTYFASDVAYHKDKFDRGFDLAID
                     VWGADHHGYIPRVRAAVEALGRDPQDFQVILIQMVNLLRHGEPVTMSKRSGQFVTLRD
                     VLDDVGPDATRYFFLMRRSDSHLDFDLGVAKTQGADNPVYYVQYAHARMASIFREAEA
                     RGIPVPSGVDPLLTEAFGSPEEQALMRQIALFPDEVAKASREYEPHRLVFFAYDLACA
                     FHSFYNASRILGEEPALRDARLVAVRASQIALANALRLLGIGAPERM"
     gene            complement(21806..23629)
                     /locus_tag="PL162_04720"
     CDS             complement(21806..23629)
                     /locus_tag="PL162_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91357.1"
                     /translation="MIKSMTRGWFWGILLSLLGAAGALGAPAPEELLAKEVRRLAREV
                     PPGGGEGSGMVWQRRSEYELQADGSLVRRSRWIVELPSRGAEAWRLWDIPVPVGGKAE
                     LLASGLYDAQTGRPLAPLSVENDDEGGIASLRVRAPRHDGAVVALEYLQVFPRRMNVD
                     DVVWLALDLPQWEQVITVRVPQGSAFFWKGVEAPAPEVTDEGPWESYEWRVVNRPAWP
                     GMGLLSQGRPVLTFSLRKGPREALTPLDYLESLPLPEPKGAFARALRGANRIAAGKRL
                     LGLMNGSSTLLEGAPWDAVRPGELISPEGPWTSWERVLLLRRWLEKVGWKATLWWIPV
                     LDLGDDAPATARAWLRPVLELKPSGGGEFFWDVGQNVAPGEVPSALWGATILRREGAR
                     VERRSMGKGALETHRFSVEWTLSLDQEGVARGSVDVTLRGGWVGIFAPQGVPEADAVK
                     SQIRWPSGAGRPVLGEATRTVYPYGYRFSFPVTVPLGIPGPGRVLVRFPGVDLPWTAD
                     LRRAAAPCALRFPFVFEQRFSLALPKGYRVLDLPKGGERGRRVVLKETVRYREKYHEV
                     VGESRLLVKDVRLGDPELAELESALAAEARWGGHQIPLMKK"
     gene            complement(23704..26376)
                     /gene="secA"
                     /locus_tag="PL162_04725"
     CDS             complement(23704..26376)
                     /gene="secA"
                     /locus_tag="PL162_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HQF91358.1"
                     /translation="MFEGLKKALGLDPNENALKRYRQRVGRINALEPALQALSDEALR
                     ARGDEFRRRIEGGEAPDALLEEVFATVREVSVRTMGLRHFDVQLMGAMALTEGCIAEM
                     KTGEGKTLVSTLAVALGGFSRQGVHVVTVNDYLAKRDAEWMGPIYRFLGLSVGVLSPF
                     MPPEERFAAYRADVTYGTNSEFGFDYLRDNMATHPSHLVQRGHAFAIVDEVDSILVDE
                     ARTPLIISGPSEDNVEPYRRANDVARRLTRDTDFEVEEKDRNVALTEGGIARCEQILQ
                     VPGLFADLARSEMAHKILQALKAWHLFQRDVHYVVKDGEIVIVDEFTGRLMFGRRYSD
                     GLHQAIEAKEGVRIGRENQTLATVTIQNYFRMYRKLAGMTGTAATEAEEFKEIYGLPV
                     VVLPTHRPMIRQDHADLIYRTKREKFAAVADEVGECHAKGQPVLVGTTSIENSEVVSK
                     LLKARKIPHQVLNAKFHEKEAQIVAQAGRFGAVTVATNMAGRGTDIVLGGNADFLSRE
                     EAPRRGVDPAADLEGFDALRRELAAQCAVEREQVLAAGGLHILGTERHESRRIDNQLR
                     GRSGRQGDPGSSRFYLALEDDLLRLFGSERIQGVMEKLGMEEGESIEHPMLTKAIANA
                     QKKVEEMHFDIRRQLLMYDNVMNQQREAVYGERNQVLREEGIRERVEEIIADAIDGVL
                     EKAFPEQGECQPRSAVLRLKGMMGPWVEPLLEGVETPQALPPAQESLKEGALERFRSK
                     MADLGPEMGEGLIRFLVLHVLDAQWKDHLLGMDELRRGIGLRAIGQKDPLLEYQFESY
                     NLFQEMLERVREGVTEYVFRVSVAAPEGDPRGQPRHAVPEILAAGGLGQDRRPDPRQG
                     ESPKPVQKGARVGPNAPCPCGSGRKYKQCCGRLA"
     gene            complement(26546..27457)
                     /locus_tag="PL162_04730"
     CDS             complement(26546..27457)
                     /locus_tag="PL162_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQF91359.1"
                     /translation="MIELFRASIRMMTPLILAALGGCWTLQTGILNISQEGAMLLGAF
                     FAAMGNHFMESWVGGLFCGVAAAVAFNLLFALFCVNLRANIWVVGMTLNILADSLSVL
                     LLKSIFHVKGYFSSPFMEKVPDVDLGWLSAPLDRVVGDFSLVVWGTLFLVLAMLWVDR
                     KAVLGLRLKAAGENEEALAAAGVSVLRLRYFALVLNGVLVGAAGSFLSISYLTLFNQG
                     MSSGRGWIAVAAVIFGDGKLGTTLGAAAAFGLVQAGGNALQNAGVNSNGTLMLPYVCI
                     LGALFWKALRARRERLLRERREIPSGA"
     gene            complement(27460..28503)
                     /locus_tag="PL162_04735"
     CDS             complement(27460..28503)
                     /locus_tag="PL162_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQF91360.1"
                     /translation="MNAFWLRHRDPLIILLSFLGALGVGGALFLAHGVNPLEAYGAMV
                     VGALGSAPALWSTLARTVPYILGALSIAVAFAAGVWNIGAEGQLYMGALGAALAGLFL
                     PPMPSLPAMILVLAFGVSVGVFWAWVPGRLSLDLGMNVVVVTIMFNAVAQYFTSYLAT
                     GPLAGKNVSAGMTDRVQDFVRFSRISDLAVLHTGVFVALGAALLVTGLMLWTAWGYEG
                     RMVRLNPRAAAAGGIPVRRRQLGAMALSGALAGLAGSLLVMGDTGRFLVGISKGYTWT
                     GMILAMMVGYNPLGVLGAALVYAAMSSGALEMELLTGIPAEVVDLIVTTSVLFVTAGM
                     AVANRLANRLRED"
     gene            complement(28518..29342)
                     /locus_tag="PL162_04740"
     CDS             complement(28518..29342)
                     /locus_tag="PL162_04740"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HQF91361.1"
                     /translation="MMRVFLSCDMEGATGVVSFDQVSRNKPEYEFGREMQLKDTWAVV
                     TAAREAGAQRIVVNDSHDAMRNLDGRALPREVELISGSPKILCMMEGVSEADVALFVA
                     YHAMGGTERALLDHTMSSTTVHEVRLNGRAVGEIGLGAALCGALGVPVALVTGDVAAC
                     IEAESLLPGVVTCPVKEGHGRFAARLLAPEVTTERLAQATREALARFREGAFKPWCPG
                     PPYELEVTFSWTSQCDKASWALPFHRVGGRTLRAEHEDFLELHRAFLAAVTLAGAV"
     gene            complement(29358..30407)
                     /locus_tag="PL162_04745"
     CDS             complement(29358..30407)
                     /locus_tag="PL162_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HQF91362.1"
                     /translation="MAMNVASWRCFEPVELRGVRLRNRFVAAPMASGTGDEESGAPGD
                     RSREFYAPLAASGVGLVVLEHAAVRADGKVRRGQFMMDQDDLVEPYRAFLKPFKEAGV
                     PVVAQLNHAGSAIKDPRLLTPGFEAVSASPVRHPRDEVGLTPRYLKEKEIFQIEEHFA
                     AAARRALAAGFAGVELHACHGFLLGQFLSPVTNRRSDSYGGGIDGRSLLLRDLVDILR
                     GVVKDAILAVRLGVSDALPGEKSPGLDVEEAVWVARQIALQGVDVVDVSGNLCGYDGP
                     GEAWWGPFARRIREACGGRPQVICSGGIRSAKTAEELLEGRVCDLVGLGRPLRQNPGL
                     VRGWEGELSPETLGR"
BASE COUNT         5339 a        10410 c         9657 g         5005 t
ORIGIN      
        1 cttggcgtgc acgggcttgc ctcccggcag gggattggtg cccgccacca gcactgcgta
       61 gaaggagaag tccagcttgt ctttggcagc ccaagccgcc ttcacgctct tcgtccggtc
      121 ggcactttcg ggccacactt cgaagaagtt cttcatgccg agccagggct tcgcggtctt
      181 gtcggcggaa gcaaagaagg cgtcggcgag gtagcccagg ttgtaccagt tgccgtcgta
      241 gccgtagaca ccgaaggtga ggcgcttggc gttccccgct tccatgaaga agtcgatggg
      301 gcccttgtta agcgccgtac catcaatatt cgccacgttg gcgatgatat cggccttggc
      361 cacggggtca cagatgtggt tctggttgag ttcgaaggtc actttcacgg gctggaagaa
      421 cgtcttgccg tcgtagatgg tgatggggca tgcggcagac caatcggccc acatgtcgag
      481 gctcaccgcc cggcagaggg tggtccccgc cggagcgcac gccgtcgtat ccgccacttg
      541 ctggaagaac tcctggctac cgcaaggatc gtcctcgccg caaatgtcgg tgagatcggc
      601 atcggcccag tagactccca tgtccttgga ggccacgccc aggtcgttgc tcagatccac
      661 cagcatggcg tcatccaccc agcgccgctc gatgcggacg caggtcgcac tgcaatccca
      721 ctcgtagcac cagttcgcat cgccccaacg cttggccaga aggttcgtag tctggcagaa
      781 gtcgttggtg gcggccgtcg catcaaagtc catgaggaca ttaagctcgg tcccattctg
      841 gtcgacgaag ttttcgccga aacggccgta gtagagcgca aagcccgggg aaaggctccg
      901 cacctgagaa gtcgtccagg aggagtgctc gtggaggaag ctgtaccagc cggcgtgagt
      961 cgccgccttg tcctggtcca tcaggtacgc aaaggccgga cgggacgcat aggcctgacc
     1021 gaggatgcgg tactggtatc cggaggcgtc agaggatgtc tctttcaccg cagaccacat
     1081 gaagcggttg tccgccagag cccgtgcaag ataagtgccc ttattgagga tgcgctccgc
     1141 cacccagtcg ccccgggcca taagccggtc gctctcggtg atgccggtga tgctggcggc
     1201 agtgggtccc cagagcttgt gggtggtgtt aaaacgctcc gtgtgcgaag tgccgtcgat
     1261 gacgtagggg ccgaggtcca gcgcatgact ccccgccacg gaagtgccat aaagcaccat
     1321 gtcgaggtag tgcacggggg tttccacgac accgtcggtc aggcagttga ggttctgcgg
     1381 catgacctgc caacgcttct cgtaggtgtc gcaggggatg tacaccgcga agtccacgga
     1441 ctccacgtcg gcagaggtgg tgaacgtgcc ggcgccgtcg cggctcatca gattgatatg
     1501 gagctttgcc gtctgcaggg tgtcacaggg gttctgaccg caagcagggc agttccactc
     1561 caccgtgaag ggccactggc gattcaggtc ataggagacg gtcccgatgg gcccaaactc
     1621 gtcaccggag tgcagactcc accccgcagc cccgttgaat cccgtgagcc agatctcgtt
     1681 ctcggtatcc acgaccgcgc tgttgtagtc cgcgctgaag gggtgggtct tggcatccac
     1741 tccggcccgg atgaagacga aatccagatc cgaagcgttg tcatagtcgg ccgccgcgtc
     1801 ggggctacgg taaacgaagt cgttccattc ccccgcgtgc tgggcgcccg aaaacccgat
     1861 gtactgcttt ccggtccggt ccgcactgta gagaaccaca cggtatccat tctggacatc
     1921 acccatggtg acgtcggtgc ccgtggtgcc ctggccgaaa tctccgggga gcaccggagt
     1981 actcactgtt actgcccaag ctcccgaggc cacgcaaagg cagagcgcca tcacgagtcc
     2041 caaaccaaaa cgtctcttca cgaagtacca ctccttccaa aaatcctcac gacgaaaaag
     2101 gacaactgcg tcttttccct gccatgtcac gccgtctcct ccgtcgcacc acccccttgg
     2161 ctccaccttc accgaaggct cccttcgggg cttttccccc ttcaagggct tcggaaattt
     2221 gacaccccgt acagcgtctc ccttttcgaa agacgcctcc tccacgcgcg actaggattt
     2281 taccccttcc tgcgcggttc gtcaattttt cggagacgat tcttgcggat atactcttcc
     2341 cgtgacggcc acaacgtagg tcgttccgtc gggggccgtg acgctccagg gggacaccgc
     2401 cccgtcggag cccacgatgc aggggccttc gcctccccac cccctgccgg gcaggggttc
     2461 tgccagaggc acgggggacc accggtcttc ctcagcgccg gggggagcgt cgggggagcg
     2521 gggggtgcgc acctccgcca ccagggtgcc ttcagccatc cgcagcctca ggggcatgcc
     2581 ccccgaggcg agctccacgg cggcccagag ggcccgctgg agggcggtgt ggggatcgga
     2641 gacgccgaag gacaggcgag ggaggatcac ggcggccaga agggccagca gggcggtgac
     2701 caccaggatc tccaccaggg tgaagccccg gcggcgaatt tttcggcgtt tcccccggag
     2761 gccctccggc ggggccatgg ggtcttctcc ctcggccatc tctttccccc ctctcgaacc
     2821 tttttttccc gatgttcccc tggggttcca ccttacttct tcccgccctc gccccttttc
     2881 ggcccccggc ggggcacgcc cggcgggaga acgcccttaa agggtcgggg aagcggggcg
     2941 gcgctcccgg gggctattcc cagttggtga tgtcggcgtc tttccccgtg cccccttctt
     3001 tgccgtcggg gccggtggag aggatgtcgt agtccccgtg ctccccgggg caccgataga
     3061 ggtaggggcg cccccagggg tcggcgggca tcttcttgag atatcccccg gcgcggtact
     3121 tccggggctc gggccccccc gcaggtttgc tcaccagggc gccgatgccc tgatccgtgg
     3181 tggggaaaaa gccgttatcc agatggaaga ggtccagggc ctgctcgatt tccttgagct
     3241 gcacctgggc ggcggtgcgg cgggcctcct ccccctgccc caacacccgg ggcaccacca
     3301 gggcggccaa aagtcccagg atgatgacca ccaccatgat ttccaccagg gtaaagcctc
     3361 gacgccgttt caaagtcttc atggatcttc gcttcctttc ttctcgtttc ttcccgtgcg
     3421 gggagctctt cggggcccgg tctcgggccc cgagggatcc ggggaaatcc cggacttgcc
     3481 tattttacca gggccgagat gtcaaagata ggcagaagca ccgccaccac cacgaagccc
     3541 acgccgcttc ccaaaaggag gatgagggcg ggacccgcga ggttcgccag gcgctccatg
     3601 cgggcctggg ccacctccca ggcgttttct gcggcgtttc tcagggcttc ggggaggtcg
     3661 ttgcccatct cccccacccg gatcatgtag accacgtcct cggtgacggg gccttctttc
     3721 tccagggcgg cggcgaagcg gtgcccctgg cgcacgaggt cggccacggc cttccatcgc
     3781 tcggggtttc ggtccagggg ggcggccatc tccaaggcct gcaccagggg gatgcccgct
     3841 tccaccaggg tggccacctg ggaaaatacc agggataggg cgatgcgctc ccgcagggcg
     3901 gcgctcccag ggatgttcag gggcttcttg cggcggcgaa gccagaaaaa cgccagcgcc
     3961 cccaggcccg ccaaggggag ccccgcccag gagacgccgt cggccacggc gagcaggatg
     4021 cgggtgggca ggggaaggac ctgccccagt tcggcgaaga gcccggcgaa tttgggcacc
     4081 acgtaggtga ggaggaagac caccaccccc aggccgaaga cgagcatgac tagggggtag
     4141 gtgagggcac tttttacctt gcggcgcagc tcgtgctcct ggcggaagag gtcggccccc
     4201 cggcgcagga tcacagggag agagccgctc tgctcccccg aagccaccat gcccaccagg
     4261 ctctcccgaa agatcccgag ttgctccatg gcggtggaca ggcgccgtcc cccctggaca
     4321 tcctccagaa gtttttcata ggccagggcc aaccgggggt cgggggactg cttggccaga
     4381 aggcgcaggg catcggtgac gggaagaccg cttttgaggt accccgccag gcttttgcag
     4441 aaaagatagt gcacgtccag aggaagggcg cgcaacgtga gcccctttcg ggccgtctcc
     4501 gtgggggcga tgtccaccac caccagcttg cgctccgcca gctgggaaag ggccccgggt
     4561 tctccggagg cctccactac gccggaaagc atctttccgg cggcgtcata ggcggtgtag
     4621 cggtatgcgg gcatggagat cctcctttct ctcgccggga tgggaatgtg gcacggcacg
     4681 aattccgccg ggctttcggg gcaaacacat ttgttgtagg ggcgaaaaat acgctcgccc
     4741 ctacacgtaa acgggcaaag atatttcacc cagatgcacc catgggcgaa ggcgtttttt
     4801 gccccttacg cctcttgccc cgccacccgg gtgagttcgt cgggggtggt ggtgccttcc
     4861 ttgacccgtt ccagtccctg ttcccacagg gtcaccagcc ctccggcccg ggccagctcc
     4921 cgcagctccg cccccggggc cccccgggcg atggcgtcgg cgagggcttc ggaggagacg
     4981 aactgttcgt agagccccac ccgccctcga taccccgtgt gacggcaatg ctcgcaccct
     5041 cgcccctgcc acactcggtc cacgccgtgg cgccgcagca ggggaggggc cggatgctcc
     5101 tcccggcacc gggggcagat gcggcgcacc aggcgctggg ccaccacgcc caccagacat
     5161 cccgccacga gatagggttc gatattcatg tccaccagcc gggccacggc ccccacgctg
     5221 tcgttggtgt ggagggtaga aaggaccaga tgtcccgtga gggaggcctg gacgccgatg
     5281 tgggcggtct cgtagtcgcg catctctccc accatgacca cgtcgggatc ttggcggagg
     5341 atggcccgga gggccgaggc gaaggtcacc ccggcttttt cgttcacctg gatctgggcc
     5401 actccgggga ggtcgtactc cacggggtcc tccacggtga tgacgttcac ggcgggttgg
     5461 gccacctgct gcaggatggc gtagagcgtg gtggtcttcc ccgatcccgt ggggcccgtg
     5521 aggaggatca tgccgtgggg ggtctcgatg atccgcccca tgatctcccg ctcccgaggg
     5581 gccatgccga gctgctccag ggtcaaaagt cccgaggatt tgtccagaag gcggagggcc
     5641 atgcgctccc cgtgctgggt ggggatggct cccacccgga tgtccaccgc ccggtccccc
     5701 agggtgatgc cgatgcgccc gtcctggggg gcgaagcggt gggcgatgtc catgcgagcc
     5761 atgaccttca ggcggctggt gaggggggcc tggtgcccct tggggaggcg caggcgttcg
     5821 tggaggaccc cgtcgatgcg gtagcgcacc accacttcgt cttcataggg ctccacgtgg
     5881 atgtccgtgg cccgctcccg cagggcttcg aggatgaggc cgttcaccag gcggatgacg
     5941 ggggcatcca cgctgtccga gaggacatcc tcccgggaga gggcggagag gtcttccacc
     6001 ccttcgacgc tccgggcggc gtcctcggcg ccggagctcc ggaggtcgta gagcttacgc
     6061 agcaccgagg cgatctcctc gggggggtgg acctccagct ccgccgcgca ccccagggag
     6121 gcggccagga gctgggccga gggccaggcc tccagcgagg aagcccccac caccagcacc
     6181 ccctcctccc ggcgcagagg gaggaggttg gtggcccgca gggtgtcgag gctcacttct
     6241 ccggggagga ggtcccggat gtcctcgggg cggggcaggg tcaattccgc ttcaccgcct
     6301 tgcgatagag ctcctccagg cgcttttgta ccagatcttc ttccgggttg ccggggacgt
     6361 cccccgagac catgtactgc cgggtgatct cggaggcctc ctgggggctt tccaggatgt
     6421 agggcgtcaa aaagatcatg aggtcgatct tttccttctg tttcaccgtg gtggtgaaga
     6481 ggttgcccac gatggggatg tacgaaagcc ccggcacacg atttttcatg gacttttccg
     6541 cctctttgat gagccctccg aggatgatgg tctggccatc ctgcaccagc accgtggtct
     6601 tcacctggcg cttggcggtg gtgggggtgg cgttggcggt ggtggtgagg acctcttcca
     6661 acgtctggtc gatgtccagg gccacgaggg tcccgctccg cacgtggggg gtcaccttga
     6721 ggatgatgcc cgtttctttg tactcgtagc tgttttgcac ggcgctgggg ttggaggtgt
     6781 ccgacaccgc ccccttgagc tgggggatga cctggcccac ctgcagggtg ctctctttgt
     6841 tgtccgtgca cagaaggcgc ggcatggaga ggacgttgat ggcgtcgtac ttgttgagga
     6901 gcttgacgta gctgtatagc agggccttgc tgtcgtattc ggtgatcttg taggagttgc
     6961 cccggtcgtc gtacttgatc tcctcgtggg tcatgaggct ccggtaccac tccaaaaacg
     7021 ccgaagggac tccgctttcg cccatgacca cttgtcccgc caccagggcg tcgccgccga
     7081 tggagccccc ccaggtggcc caatcgatcc cggcctggtt aagcttgttc aaagtgactt
     7141 ccgccaccag gccccggagg aggatctgct tgggctgaac gtcgatctgg cggatgagct
     7201 gttccatcat gacgtactgg tcctggggga cgttgaggat gatgctgttg gtgggcacgt
     7261 cggggaccac cacggtgccg atcatcccgg cgcgctcggg cagggcggcg ttcgccaggg
     7321 tgagcacctg ggtgagctgg gtcgccacgg tggtggcgtc ggcgttctgg agcttgtaga
     7381 cgtaaaatcc gctggacttg gcggggacgt ccatctcgat gagaagacgc ttcacctctt
     7441 tggcggtggt gcggtcggcc acgaagacaa gcttctggcg ggtgtcgtcc ccaatggcgg
     7501 tgacccccgt cagggcggag gccccggact gccccgcctg ggtgatgtac cccgcaatga
     7561 cggagggacg ggcgtagagg agatccacca cgtggacgtc ccgaaggttg tcggggacat
     7621 ccagggcgtg gaggacggac acggcccgct gcaccagggt ggctttgccg ctcaagatgg
     7681 cgccattgcc cgtctcggtg gggaaaaaga ccacggcgcc ccccagggcg gtgctcaccg
     7741 cgctctgggc gtaagcggcg gcgacgtact ccagggggac gacctggacg acgtattctt
     7801 cgccatagcc ggggcccgcc ttgcccttgc gcaccatgtt gtcggtgctg ggtcccgtgc
     7861 tctgggagcg gattttgctg aagcctccct cgctctggag ggccaggccg ttcatctcca
     7921 gggccgagag catgacctgg cgggactccc gcagggacac cgggcgggtg gagacgatgg
     7981 tgatgctccc cttggcgctg ggatccacca ggatgttctc ccccaggagt tcggacatga
     8041 agcggatgaa tttcaccagg tccaggtttt tgaagttgaa ctgcacctgt ccggcctgcc
     8101 tcatggcctg ggccatcttc accagctcct gctcgtcggg ctcaacccct tcctgggccc
     8161 gggcctccat cggggccggg atgagggggc tcaagggagc gagggggagg aagaggagaa
     8221 agagggccca ggccaggatg gcccctcctt ttcgccccgc cccgaagccc ccggggtgtc
     8281 cctgcgagat cgtcgctttt ttctcgtgcc tttccatgct ttatcctccg tccttcgtcc
     8341 gggagatccc ggaaaatcct cgccaggctc ccctctacgg ggcgatccgg ggcggtttag
     8401 ggtacccaga tttccagggt ccgccccgag agggtgaccg tgaagcgctt gtagggtaga
     8461 cgcagccccg tgggggggat ctccacttct tccttcaaaa ggctccagga caggtctccg
     8521 ctggagcccg agtcgggcag ggtgcccgcg cgctggatca cctggagggc ccgggcttcg
     8581 tccaggagcc tccgctggat ctggacttcg tccagggtcc gctgggagag ggccgccagg
     8641 cgcagggccc ccacgctcac cagggccaag atcgccacgg ccaccatgat ctccaccagg
     8701 gtaaagcccg acgcggcgcc ccgaagattt cttcttttta cctccggcga ggccgacctg
     8761 gtccgcgcca tgggtcgtcc cccatcccta cttgacggcg taggacagct gttccggctt
     8821 gccgtcccgc acgatggcca cgtcgaactt gctgcccccc atgagggagt tgatggcatt
     8881 ggccacgtcc cccatgttgt tcatggtgat gccgttgatc cccttgatga cgtctccggg
     8941 gcgcaccccc agggtggaga ggatgctctt atcctccagg gccgctactt ccaagccccc
     9001 agccttcccc tggtcgtcca ccttggggat cagacgcacc agtttgagct cgtcaaaggg
     9061 gttcatcaac agggcattca ccacttcccg agcgacgaca ccctccttgc ccggcgcggc
     9121 ggagctcacc ttcccctgga aggcgggggg aagggccgga ggggccgagg accccgagga
     9181 tggggaaccg gccaccgtca gggaggccgg ggcccccggg cttcccccca cgaagacgag
     9241 ggggaggaag gtctcctggc catcccggct caggaggacc cccccggcct ggacgcgaaa
     9301 gaccagatat ccccggaact cttccctctg gaggatccac cggggtcctt cttccgtgga
     9361 caccagggcc cccacccccg ccaccgtgcc ctccacctgg acgtcagcca gggagagccc
     9421 ggggccagtg gtttcggcga cggggacgga ggccaccgcc gggcgagggg ggaccccaaa
     9481 ggggtttcgc tccacgaagg cgacgacccc cggcggggcc ccccgggagg cgctctcccg
     9541 ggaccccagg acgctttgta ggacgtgctc cgtccggagg gccagggtgt gccgccggag
     9601 gaactcttct cccaaaaagg ccacccctcg ggccgccacc agggcgagga cggcccaggc
     9661 gccccagcgg accagggcga cgaggagatg ggatcctccg ccgacggcgg aggggcgaag
     9721 gatctgctta agactgggga gcgtcatggc cttcctcccg gaccaggtgc cactggccct
     9781 cggattcctt ctgcaggggc aaaaacgcgg cggcggcctc cagcaccccg tccagctctc
     9841 cgggaacctt gaggcgcata tcggcggcgc taatgcggga cgcgacgagg tccagggtga
     9901 cgtcgccccg ggcctggagc tccccccgga gctccagcgc caggaagcgc cccgtccccg
     9961 gggtcagctc cacctgggtc tgcccccctt cccaagaggc ctccttgccg aaggcctcca
    10021 ctcccccctc ttcccaggag aggacaaagg ccccccttcc ccccaggagg ggcaaagggt
    10081 tccaccggag ctccaccccc cggcttcgcg ccgagaaaag gggagagcgc ccttcgatgc
    10141 tctccagcgt cagggagggg atccaccctc ctcccgccgt ggcgtttcgc accgtaatct
    10201 cgatgccccg gcgatcgccc tctcggatca acagggccac gccccaatcc accgcctcct
    10261 ggaggggaaa gaaaagcccg agccccccga cgaagcaggc gataaaaaat atcagcggcg
    10321 gggcgatgat tttaaacctt ttcacgattc cacccccacg atcagcgaca ggacgaagag
    10381 gggaccatct ttttccggca gggtacggag ctcggcgctg cgcaccacga gccccgcccc
    10441 gtcgagttct tggaggagcg tcatgaactc ctccgcatag aggcgctcca ggaccaccac
    10501 cactccctga tccgaggcgg agagatcctt tatgcgcccc ttcatcccca ggcgatccgc
    10561 cacgccggaa aaggcctcca gggggtccga ggccctgccc tccctcaccc cgggggcgga
    10621 ggcccccccc agggcccgat attgggacac cacgtccaag aacttccgga aacggtcttc
    10681 cagcagggag gagcgctgcc actccgaaga ggcctcctgg gccagctgga accccacgct
    10741 ccaggcgacc aggaccccca gaaggagcag gacaaaacgg cgaaactcgg cgttggcccc
    10801 tagggcttca cgaaaacggc ccagggcccc ccccagggag gtcaggaaag aatcgtcccc
    10861 tttcatcaag gtttctgcca ccccacttcc acggtgaagc gcacctgtcc gtcgggggtc
    10921 tgctgcacat ctcccacccg ggaggacaat tcgccctttt taaggcgctc tttgaccttc
    10981 gccagggtcc ccacgtcggg gagcaccccg cccatctggg cccccgaggc gctgtagcga
    11041 agggtttcca gggtcatctt atcccctccc tcggagaggg tctcccccac gaagcggatc
    11101 acgtccccca gggaggcccc ccgggcttga cggttctccc cccgggcctc ggccagacgg
    11161 gcccgggcct ggctcaaggg gtcggtggga gtgccgccaa agacctgggc gtaggcctcc
    11221 gccgcccggg cctggagatc ttcttggcgg tgcagggcac tccagagctc gcccccctgt
    11281 acccccgaaa agagcaaccc ccccaccagg agggctccca gggggccccg aagggacaaa
    11341 agggcctgat cccaggccac cagggcatcc actccccccg atgagaggtc cacctcgtgc
    11401 aggaggggaa aggccttcca agtggcgtct gcggcggccc ccaggcgccg cagggcctct
    11461 tggggggtcc ggggatccag ggtctcctcc acgaaggaga acgcctctcc ccccatctgg
    11521 cgaaaccagg ccttctcccc ctccaggccc tggggtccca gggtcttcca tcgggccaag
    11581 aagggaatcc cctgacgcca ggcaagggaa gaaaccgccc ccccgtcaac ccagagggcc
    11641 gccccgtcgc cttccatctg ggaggccaag gccaggggca ggggccaaac ggtgaggcga
    11701 ggtccccggc gcccttcgag aaaggcgagc tcctcccggg accagaacca ggccaccccc
    11761 gaggccccct ttccctcgcg ccgccacacc gtgggaagga cttccacccc ctgccctccc
    11821 agcaggaggc tccggtactg aagggccagg gcctcccgga tccgcgacag gttgccgaag
    11881 ggataggaaa aggggtggac ggagagcgtc cgaaagggat agaggaggac gccggaaaag
    11941 tcctccggga gcccctcgtg gcgcaccacc gatccctggc gcaagatcca gtaccccccg
    12001 gccccgggaa cgccccagga agcaagatgc cttcgcccca tcaatgtctc tcctcccaag
    12061 ccaccgccct cacggtctgg tggtctttct tcaagacgat atcataaacc caagagcgct
    12121 ttcccgccca cgtcacttcc acggtcaggc caaagtagga gctttttacc cccaccagat
    12181 tcatcacgct ccccaaggcc tcccggggaa aggccggggc ctcgaagaag tcctcgatct
    12241 ttttgaaggg gtgatcgacc cggtaggctc ccagggcccg ggccccctcg ggggtgaagc
    12301 cctcgtgcaa aagggtgagc acgtcctccg atgcggtgtt gacatttatc ttgccatcgc
    12361 accacacggt gagaaagtcc tccagccctt ttttcgaagg tcccgagggg ccttttagga
    12421 ggggcggaga gaggccaggg cacaggagca actccgacag atcccccagg gggcgattga
    12481 tgaagccctc cctctcccct ccccccagac gcggggtgcc gtcggcgtcg aggaaatcga
    12541 gcaccaccgg ggccagctcc ccgtggccca gcctattcca gagctcctcc caggggtgaa
    12601 tcatctcctg cctcagggtg gtgccgtcgg gaaggaaaag agcgttcacc ggaagggccc
    12661 ggtccagggg gacgaggtgc aaagacacca caccctcccc ggcgggaaag gccatgggct
    12721 gaaactgagg cttgtccggg gcgtcgtaat cgctccggtc ggcggccaac ccccgggcca
    12781 cgaagaaggc cgtgagaaag gcaaggttcc gagaccggag ggcggtgcgc tccccctcca
    12841 cccggcggag ctggtttcgg gcgaaccagg cgaagcccgt ggtggcggca atgagcacca
    12901 cggagatcat gagcaccgcc gcgaggacga aggccgggcg cctactgcgg gagccagtcc
    12961 acataggtca gggcctttcc gtcgcgttcc aggtgtattt ccacccccgg ggggaactct
    13021 ccttcgtact gccccaccca ggcggcgccg tcatgcaccc ggaggcgcaa cgccgtcaca
    13081 tcggggagca ccagctgtcc ccggtccaaa gacacaccgt cggggtccac ctggtggggc
    13141 aaaagccccc cgaggaacca ccgatatagc cccggtatca tagccgaaaa ggggtcgtcg
    13201 ggccagatca cccggtaaag gacgctcccc tggggccggt ctcccccccg ggaaaccccg
    13261 ctccaggcca ccaccgcgtc gttggcggca ccaccgaact tgtccctccg gatgagccga
    13321 aaggcggggc cctggggcac gggcaaaagg ctctgccaat cccccgccat gcgggaagcg
    13381 gccaggcgaa aggccgtgtc ctcggcaaag tcatcctcca cccgggtgag gagtcccacg
    13441 gtgtgcacca ccggggcgat gcccacggcg gccacgatcc ccaccagggc cagtaccagg
    13501 aggatctcca ccagagtaaa gccccggggg ggcctccggg cccccccggg ggaggaaaga
    13561 atcaaggctc gacgctctcc gcgctcatcg gaggaggggc ggaggccccg ggagcgggga
    13621 cgacctgata ggatccgggc tggagatagg ccgtgaaacg gtcgatcatc tcccggctgt
    13681 ggaggcgatc ggcccactgg agtagggtca tgagatcctc ggcggtggac tcccggtaga
    13741 agtactgagt caggcggttg agcccctcca ggaggaggtc gccatcctga ctggattctc
    13801 ccgcccggat gaggaggatc gccagctggc gctcggcgtc cagatggacc atgtagtgat
    13861 cctgggcctt ttccaggagc cttttatact cctgggcgtt ggaagcccgg agggagcggg
    13921 ccaggatctt gtccccgacc atgccgtccc cgaggaagca gagccccccc agggccgcca
    13981 ccgccgcaaa gccccccaga aagcgcccca ccaggggcat cctccccagg gtcccccggt
    14041 cttcctccga agggagaagg atctcccggg cggataggcc cagagccagg gcgagccaca
    14101 ccgcatcttc gatgcggtgt agcggtctat cccagaccat ggcaaaaaaa tagctggtga
    14161 cgaagccgca ggcccacacc gccggaggag agagcttctg acgccgcccc aaggcccggc
    14221 agaagcgcca gagccaccat aggaccatca gagaaaggat ggcccccccc acgatccccc
    14281 cttcggcgaa ccactggaga tattcgttgt gggcccagtt ggtgaactgg aattcgtttt
    14341 ccgggaagtc gcgaaacatc tcccgttggg cgtcgaggta gtgccacttg tagtgcccca
    14401 gccccacccc ctcggggtgc tggcggatca tggtccagga ggtggcccag atggcgctgc
    14461 gccccccgat gcccttgtag ttcaccacca tgtccccgaa cttatagaaa aagacatcgg
    14521 ccccccggac ccccatcatg gccacgccgt aggcgaggaa gaaggccccg gtgaaaataa
    14581 gcaccctctt taacactcgc cgccgatcct cggcctgggc ccaaaaggcc acccccaggg
    14641 tcaaaagccc cacgaggaag gccaagaagg ccgaccggct ggtggaagcg gccaggccat
    14701 acacgtccac cgccaaaagc cccagggcga cccaccagaa aaaggggtgg gatccgtcct
    14761 cccggcggaa ggccccggtg aggaagcacc aacacatgcc gtaggccccc acgaacatcc
    14821 aaaggcccat catgttctgg taccccgtgt tgccaatgta aaatcccggg gtatgtagta
    14881 tgaagaaaag atcaggggtc accaggtctc gcatctggag ctcggcaaag cccacgttga
    14941 tggccccgtt cagcgccgcc cccaagagga tccagcgcag gtcccggcgc catcgcccgg
    15001 ggtccgtgtt tagggccagg aggtacaccc cgagcatggt ggcgaggaaa aaccactcct
    15061 tcaccagcgt gggtcccgag gtgatcggaa cccagagggg ttggagggca aagtaaagca
    15121 gcatgaaaaa ccatgccagc ccgaaggcgt cccaccggaa gttcaaccga tccgccccgt
    15181 accacgccac ccggctcccc gccacgaccc agcagagggc cacgggaacg gcggtcacca
    15241 cccacttcat gaggtgcagc gtgtcaaacc acgataggcc ggaaaagacc aggttgggca
    15301 ccgcgagggc aaccaaaagg aggggaaagg tcatccacca cggcgccaga gccgggggag
    15361 agggggaaat catcggcccc gggggagcct tgcgaagcac cttgttcttg gccacggaaa
    15421 aatcctcctt aaaaattgaa cgtcccgccc ttcggttccc ggggggaggg cttttttcgc
    15481 cccaccccga gctccgtcac cccctcgggc ggcggaacca ggggcattat gtcaccggcc
    15541 gatccccggg ggcaacggta attccacgca ggggaccgca tgagggagcc ctagggtagg
    15601 tacggccgcc tttcggagct cacgaactgg gcaaactctt tcataaaaat tagatttatc
    15661 gtccccgtgg ggccgttgcg gtgtttggcc aggatgacct cggcccgggg gtcttcctcg
    15721 tcgccgtctt ttcggtcgta gtatccgggg cgatagagca aaagtaccat gtcggcgtcc
    15781 tgttcgatgg cgccgctgtc ccggaggtct gacagcatgg ggcgccggtc ggtgcgcgac
    15841 tccacggccc gggagagctg ggagatggcc accaccggca ccgatagctc ccgggccacg
    15901 gccttgaggg cccgggatat ttcggccacc tcctgctggc ggctctccgc agaccgaagg
    15961 ctgcgcatga gctggaggta gtccaccacc acgagcccca caggctggcc cttcatgagg
    16021 gccacaaagc gccgggaccg ggcccggagg tccagggtgg agaggtcgga gctgtcgtcg
    16081 atgtagatgg gagcggcgga aatgcgcccc gccgcgtggg ccagacgatc ccactgatcc
    16141 cgggagaggt ttcccgtccg gaggtcgtgg atgtttatct gggcctccga tcccaaaagg
    16201 cggtgcacca gctgctctcc ccccatctcc aggctgaaga tgaggaccgg cttcttttcc
    16261 ttgatggccg catgctgggc catgttcagg gcaaaggccg tcttccccat ggcggggcgg
    16321 gccgccacca cgatgaggct cccgggctgg agccctcctc ccaggtgatc gaagtccacg
    16381 aagcccgagg gggtgccgat ggtctggtcc ttgcggagaa aggccttttc gatgtgctga
    16441 aaggcctcgg ggacgatctc ccggatgtgg cgaaaggggc ggtcgttccc cctctgggca
    16501 atgtcgaaga ccaggcgctc ggcctcgtcg aggacgtctt ccatctcccg gtcttcggca
    16561 aagcccaggc gggagatgag ccccccggcg gcgatgagac gccgcctcag ggccttgttc
    16621 cgcacgatcc gggcgtgcca ctccacgttg gcggtggtgg ggacgctgtc caccacggcg
    16681 gcgaggaagc cctgcccccc caggcgctcc gccaggcccg catggcggcc gatctcctcc
    16741 catagggtca ggggatccac gggaagatcc ttgcgggcca tctcccccac caaggagaag
    16801 acgtcccggt ggtgcaggtc gtaaaagtcc tcaggatcca agatctccgt ggcggaaagg
    16861 agggcatccc gatccagcag gcaggccccc aggaccgccc gctccgcctc caagctgtgg
    16921 ggctggactc gatcgaaaag ggattcgacc accctactcc gcctcgacgc tcacggtgag
    16981 ggaggtctct acccccgcat ggaggcgcac cgtcacgggg taaacgccca cggagcggag
    17041 ggcctcgggg agccggaggt cctttttgtc caccttggcc cccagttggg tcgcgatggc
    17101 ctcggccacc tgggcagagg tgacgctgcc gaagagcttt ccggcgtctc ccgcgctggc
    17161 tttcaccaca acgcgcttgc cctggaggtg gcgccccact tcccgggcat gctgctccgc
    17221 cttctccgcc ttggcccttt tggtgtcttc ctggttctcc cagtctttca ccttccccgc
    17281 cgtgccctct tccgccagct ggcgggggaa gaggaagttc cgggcatagc cgtcggagac
    17341 ctccaccaga tctccttttt tgccgagctt ggccacgtct tgtttaagaa tgactttcat
    17401 ctctttcacc tcgcaggaag ttctccccgg ggcctgggag ttttaggggg ctcactctac
    17461 cccccgggcc cggaagtcaa accacagatc cgcgatcccc agcaccgtgg cgccctggga
    17521 caggaagggg aaggccatca ccaggaaaaa ggccacccac cggagcttac cccagcgcca
    17581 gcgtcgcaaa aaggccccca ggaccgccat gccctgcaac agaaaaagca tggtcaccac
    17641 catgcgcagg ttgattcccg cccggaagag gatgccccgc ccctcccccc ctccgtccag
    17701 gaggggaaac accagggagg ccaaaagggc ccacaccagg ctgcggggaa aacgccaggt
    17761 gtcaaagaga gggatggggg gcaggcgccg ctgtcccagg cgctggagca cggcggcgct
    17821 cacggcgtag ctgagccaac agtccagggc ccccgccatg accaaaaggg cggggaagat
    17881 gagggggatg atctgaaaac tggcctccat ctgggcccgc acgggctcca gggccgccgg
    17941 ggctccctgc cctccccatc cgcccaccag gtcgaaggcc tgctgcatgg tgaggcgcat
    18001 ctgatcgggg tcgacggaaa aggggttaat gccggtgacc gccgtggcga ctcccatgag
    18061 gaggagcttg ctggccagag acacgaggac gccgtagagg aggatctcct ccgcatggtc
    18121 gtactttttc gccaggactc cgagccccac ccccagcact ccgaagccca gggtgaaaaa
    18181 tagtcccccc aggggcccag aaaagatcac cgtcaggatc cccgccaccc cggtgcccag
    18241 gatggcgtgg cgcagggagt ggcggaggcc cagcaccacc aggggagtgg ggcagatcag
    18301 cgccgccacg acgcccacca gggggagggc ggagctgatg agaaagagca ccgccgccaa
    18361 ggcggccaac agggcggatt ccaccaaggg gcgggggtca tgcatggccc gtcatccttt
    18421 cctccacgtc ggcgaaaggg ggacatccct tcccgttcac cgctccagaa cgccgcgagg
    18481 gagaggacgg gcctctccct tcgctcgaaa aaactcccgt ctactccgca gagaagggta
    18541 cgagggccat catccgggcc cgcttgatgg cgtcggtgag gcgccgctgg tgcttggcgc
    18601 aatttcccga aacccgccgg gggaggatct tgcctcgctc ggagatgtac ttgcggagct
    18661 tttccacgtc tttgtagtcc acgtggtcta ctttatctac acagaaaaaa cacaccttgg
    18721 gacggcgctt gccgcgcttg cgcggccccc ccgccggccc accatgacca tgggatggtg
    18781 cagccatgaa cgttatcctc ctttttgcta aaacctttgc taaaaccgcc ggaccactcc
    18841 ctagaagggg atgtcagctt cctctcctcc ccggtcttcg ggcatgtcgg agatatccag
    18901 gggaaagtct tcttccgtga accctttttc gtcgcgaagg ctgccaaagc gaggcggacg
    18961 ggtggcgggt tcgtcccgat ggggccctcc ccgggagggg gcggcgcccc cttcgtcccg
    19021 gcgacccccg atgaactgga tcgactgggc caccacctcg gtggcgaagc gcttctgtcc
    19081 gtctttttcg tagctccgca cggagatgcg cccctccaca aggacccccg agcccttgga
    19141 aagatactgc tcgcagtgct ccgcttggat gccccagacc gatacgggga tgaaatccac
    19201 ctgttcctga acttcgccat tggatccctt ccaccggcgg ttcaccgcca cggtgatccg
    19261 ggccaccgcc tgtttggagg cggtgtaacg cacctcgggg tctcgggcga ggttccccat
    19321 gatgatggcc ttgttgaatc cgcgcacggc actccctcct tagagctcgt ccttacgcac
    19381 caccatgtgc cgcatgacgt tcgtccggat gtgcagcagg cgatctagtt ccttcacctg
    19441 ccccggatcc agagtcagct ggtagagggt atagtatccc tcctgcttct tgtcgacggg
    19501 gtaggcaagc cttcgcttgc cccagtgatc gatcttggac acttctcccc cgacaccctt
    19561 gacaacttca ccgatctcct ccgccaggac cttgtggtcc tcgacgtcgg ggtggaacag
    19621 caccatcatt tcgtagggcc gcaccacctt cacctcctcc ctttggactt gaggcctctt
    19681 ctcgcgcgga agaggcaggg acacacgcga ctttggatta tatcctaggg gccgtctctc
    19741 gacaagatgc gcacctcatc tcccgacgtg taaacgacgc gaaagaccgt ttcgtcgggg
    19801 taaagtaggt taaattgctc ccgggccaac cgggccactc cctccggggt ctcgtaatag
    19861 gcaatctcct cccgcaacgt ctgcatccgg cgactgaggc gcacgatctc gtccatgcgc
    19921 tgatccacca ggtaggagag cctggcgatg cgcgccagct caaagccgta ggccgtgagc
    19981 atcacggcaa agcagagtcc ggtgacggtg aggacaaaaa cccatcggag cctcggcatg
    20041 gcgcccccga agctacatgc gctccggagc cccgatgccc aagaggcgca gggcattggc
    20101 cagggcgatc tggctcgccc gcaccgccac caggcgggcg tcccgcaggg cgggctcttc
    20161 ccccaaaatc cgggaggcgt tgtagaacga gtggaaggcg caggccaggt cgtaggcgaa
    20221 aaacaccaga cggtggggct catactcccg ggaggccttg gccacttcgt cggggaagag
    20281 ggcaatctgg cgcatgaggg cctgctcctc gggggagccg aaggcttcgg tcaagagggg
    20341 atccactccg gagggcacgg ggatcccccg ggcctcggcc tcccggaaga tgctggccat
    20401 gcgggcatgg gcgtactgga cgtaatacac gggattgtcc gctccctggg tcttggccac
    20461 tcccaggtca aaatccaggt ggctgtccga ccggcgcatg aggaaaaagt agcgcgtggc
    20521 gtccggtccc acgtcgtcca acacgtcccg tagggtgacg aactgccccg agcgcttgga
    20581 catggtgacg ggctccccgt ggcgcaggag gttcaccatc tggatcagga tcacctggaa
    20641 atcctgggga tccctcccca gggcctccac ggcggcccgg acccggggaa tgtatccatg
    20701 gtgatccgcc ccccagacgt cgatggccaa gtcaaacccc cggtcgaact tgtccttgtg
    20761 gtaggccacg tcggaggcga agtaggtggg ggctccgttg ctgcggatga gcacccggtc
    20821 cttttcatcc ccgaaggccg tggcccggaa ccacacggcc ccatcctggt cgtagacgaa
    20881 gccccgcccc cggaggtgat cgatcatgcc ggggaccgag gcgttgtcgt agaggctttt
    20941 ttccgaaaac cacacgtcga agcgcacccc gaagaactcc aggtcctttc ggatgacctc
    21001 cagaagggtg cggcagccgt actcccggaa gaagggcagg gtttcctcca ggggaagatc
    21061 caggaggtgg ggcccttctt ggtccaacac gccctgggcc acgtcccgga tgtaatctcc
    21121 gtggtacccg tcttcgggga agggggcccg ctctccctgg cccaggagct cgaagtaacg
    21181 ggactggacg gagcgcccca ggttgtccac ctggagcccc gcgtcgttga tgtagtactc
    21241 ccgggccacc tgccacccgg tgaactccag gatggacgtc atcacgtctc ccaccacggc
    21301 gccccgaccg tgacccacgt gaagggggcc cgtggggttg gcgctcacga actccacctg
    21361 tacccggcgg cccctcccca ggtcggaacg gccgtaccgc tcgccctcgg agaacacacg
    21421 ctccgccacg gcgcccatcc acccccggga aaggcgaaag tttatgaacc cggggcccgc
    21481 gatctccacc ccggccacgc agctcttctc gccgggggtg aggcgctccg ccagggcctg
    21541 ggccacctcc cggggatggc ttccgaagac tttagccccc tgaagggcca cgttggtggc
    21601 ccaatctccc tggtccgggc gtttggggcg ttccaggtac acttcaggga ggagctccgg
    21661 agccaccccc cggtcccggg ccaggtcctc caggacctta gagatctcct gttttaacat
    21721 gtcgcgaatg tcttccacgg gaaaagccct cctctttcct gccccaggac ggggcaaccc
    21781 ttccctccgg gggctccgaa gggatttact ttttcatcaa ggggatctgg tgccctcccc
    21841 accgagcttc ggcggccagg gcgctttcca gctccgccag ctccggatcc cccaggcgca
    21901 cgtctttcac cagaaggcgg ctctccccca ccacttcgtg gtacttctcc cgataccgca
    21961 ccgtctcctt gagcaccacc cgccgtcccc gctccccccc cttggggagg tccaaaacgc
    22021 ggtaaccctt gggaagggcc agggagaatc gttgctcgaa gacgaaggga aaccgcaggg
    22081 cacagggggc ggcggcgcgc cggaggtcgg cggtccaggg gaggtccact ccgggaaagc
    22141 gcaccaacac ccgtccgggg cccgggatgc ccaaagggac ggtgaccgga aaggaaaagc
    22201 gataaccgta ggggtagacg gtcctggtgg cctctcccag cacgggacgc cccgccccgg
    22261 agggccatcg gatctggctc ttcaccgcat cggcctccgg cacgccctgg ggggcgaaga
    22321 tccccaccca ccccccccga agggtgacgt ccaccgatcc ccgggccacc ccttcctggt
    22381 ccaggctcag ggtccactcc accgagaagc gatgggtctc caaggcgccc tttcccatgg
    22441 atcgccgctc cacccgggcg ccctcccgac gaaggatcgt ggcaccccaa agggccgagg
    22501 ggacctctcc cggggccacg ttctgcccca cgtcccagaa aaactccccg cccccggagg
    22561 gtttcagttc cagcaccgga cggagccagg cccgggccgt ggcgggggcg tcatccccca
    22621 ggtccagcac cgggatccac cacagggtgg ccttccaccc caccttctca agccaccgcc
    22681 ggagcaggag cacccgctcc catgaggtcc agggcccctc cggagagatg agctccccgg
    22741 ggcgcaccgc atcccacggg gctccctcca gaagggtgga ggagccattc atgagcccca
    22801 ggagcctttt ccccgcagcg atgcggttgg ccccccgtag cgcccgggcg aaggcccctt
    22861 tcggttcggg caggggaagg ctctccaggt agtccagggg cgtcagggcc tcgcggggtc
    22921 cctttctcag gctgaaagtc agcacggggc gcccctggga caggagcccc atccccggcc
    22981 aggcggggcg gttcaccacc cgccactcat agctttccca gggcccctcg tcggtgacct
    23041 cgggggccgg agcctccact cccttccaga aaaaggcgct cccctggggg acacgcaccg
    23101 tgatcacctg ctcccactgg ggaaggtcca gggcgagcca caccacgtcg tccacgttca
    23161 tccgtcgcgg aaagacctgg aggtattcca gcgccaccac cgcgccgtcg tgccgggggg
    23221 cgcgcaccct cagcgaggca atgccccctt cgtcgtcatt ttccaccgaa aggggggcca
    23281 ggggacgccc cgtctgggcg tcgtagaggc cgctcgccaa aagctcggcc tttcccccca
    23341 cgggaacggg gatatcccag aggcgccagg cctccgcccc ccgggagggg agctcgacga
    23401 tccatcggga acgccgcacc aaggagccgt ccgcttgtag ctcgtactcc gaccgtcgct
    23461 gccacaccat gcccgatcct tccccccctc cggggggcac ttctcgggcc aggcggcgca
    23521 cttctttcgc cagtagctct tccggcgccg gagcccccag ggcccccgcc gctcccagca
    23581 gagaaaggag gatcccccag aaccaccccc gggtcatgct ttttatcatc gccttttcct
    23641 cctctcaccc tcgcgccacc gccccaaggg ccgaggactc caagggtcgt tccggcttgg
    23701 ggatcacgcc agccggccgc agcattgttt gtacttgcgc ccgctcccgc agggacacgg
    23761 ggcgttgggt cccacccggg cccccttttg aacgggcttg gggctctccc cctggcgagg
    23821 atcggggcgc cgatcctgcc ccagcccccc cgccgccagg atctccggca ccgcgtggcg
    23881 gggctggccc cgaggatccc cttccggggc cgccacggag acccgaaaga cgtactccgt
    23941 cacgccctct cggacgcgct ccaacatctc ctggaagagg ttgtaggact caaactggta
    24001 ctccaggagg ggatctttct ggccgatggc ccggaggcct atgccgcgcc gaagctcgtc
    24061 catccccagg aggtggtcct tccactgggc gtccaagacg tgcagcacga gaaagcgaat
    24121 cagtccctcg cccatctcgg gccccagatc ggccatcttg ctccggaagc gctccagggc
    24181 cccttccttg agggattcct gggccggggg caaagcctgg ggggtctcca ccccctccag
    24241 gaggggttcc acccaggggc ccatcatgcc cttgagccga agcaccgccg accggggttg
    24301 gcactccccc tgctcgggaa aggccttttc cagcacaccg tcgatggcgt cggcgatgat
    24361 ttcttccacc cgctcccgga ttccctcttc ccgcaggacc tgatttcgct cgccgtagac
    24421 cgcctcgcgc tgctggttca tgacgttatc gtacatgagg agctggcggc ggatgtcgaa
    24481 gtgcatctcc tccacctttt tctgggcatt ggcaatggcc ttggtgagca tggggtgctc
    24541 gatggattcg ccctcttcca tgcccagctt ctccatgacg ccctggatgc gctccgagcc
    24601 gaaaaggcgc aggaggtcgt cttccagggc gaggtagaag cggctggagc ccggatctcc
    24661 ctggcggccg ctacgccccc ggagctggtt gtcgatgcgc cgggactcgt ggcgctcggt
    24721 gcccaggatg tgaaggcccc cggcggccaa cacctgctcc cgctcgacgg cgcactgggc
    24781 ggccagctcc cggcggaggg catcaaagcc ctcgaggtcg gcggcgggat ccacgccccg
    24841 ccggggggcc tcttcccggg acaaaaagtc cgcgttgccc cccagaacga tgtccgtgcc
    24901 gcgccccgcc atattggtgg ccaccgtgac ggcgccgaag cgccccgcct gggccacgat
    24961 ctgggcttct ttttcgtgga acttggcgtt caacacctga tgggggatct tgcgggcctt
    25021 gaggagctta ctcaccactt cggagttctc gatggaggtg gtgcccacca gcaccggctg
    25081 ccctttggcg tgacactctc ccacctcgtc cgccacagcg gcgaacttct cccgcttggt
    25141 gcggtagatc agatcggcgt gatcctgacg gatcatggga cggtgcgtgg ggagcaccac
    25201 cacggggaga ccgtagatct ctttgaactc ctccgcttcc gtggcggcgg tccccgtcat
    25261 acccgccagc ttgcggtaca tgcggaagta gttctggatg gtcaccgtgg cgagggtctg
    25321 attttcccgg ccaatgcgca ctccctcttt ggcctcgatg gcctggtgga ggccgtcgga
    25381 gtaccggcga ccgaacatga gccgaccggt gaactcgtcc acgatgacga tctccccgtc
    25441 tttcaccacg tagtgcacgt cgcgctggaa aagatgccat gccttcaggg cctggaggat
    25501 cttatgggcc atctcggagc gggccagatc ggcaaagagc cccggcacct ggaggatctg
    25561 ttcgcagcgg gcgatgcctc cttcggtgag ggccacgttg cggtcctttt cttccacctc
    25621 gaagtccgtg tcccgggtga ggcgccgggc cacatcgttg gcccgacgat agggctccac
    25681 gttgtcctcc gatggtcccg agatgatgag gggcgtccgg gcctcgtcca ccaggatgga
    25741 gtccacctcg tccacgatgg cgaaggcatg cccccgttgt accaggtgcg atgggtgggt
    25801 ggccatgttg tcccgcaggt agtcgaagcc aaattcgctg ttggtgccgt aggtcacgtc
    25861 cgcccgataa gcggcgaagc gctcctctgg gggcatgaag ggggagagga ctcccacgga
    25921 aagccccagg aagcgataga tgggccccat ccactccgcg tcccgcttgg ccaggtagtc
    25981 gttcaccgtc accacgtgca cgccctggcg agaaaagccc cccagggcca ccgccagggt
    26041 ggataccaag gtcttccctt cgccggtttt catctcggcg atgcaccctt cggtgagcgc
    26101 catggctccc atgagctgta cgtcgaagtg gcgcaacccc atggtgcgca cggagacctc
    26161 ccgcaccgtg gcgaagacct cttccagcag ggcgtcgggg gcctctcctc cttcgatgcg
    26221 ccgccggaac tcgtctcccc gggcccggag ggcctcgtcg gagagggcct ggagggcggg
    26281 ctccagggcg ttgatccgcc ccactcgctg ccgatagcgc ttcagggcgt tctcgttggg
    26341 atcgagcccc aacgccttct tcaaaccttc gaacatgccg tcacctcata ttttgtctcc
    26401 ggcttctcga tttccgccgg gagaactcgc gtcccgggcg attataggcc cgatgttttc
    26461 tctgcgtcaa aagttttcct tcccttgagc ctccgcccgg aggaccgatc tccacaccgg
    26521 ggcgaaaggg agccggaggg gcgccctagg cgccggaggg gatctcccgc cgttcccgca
    26581 ggagccgctc cctccgggcg cgcagggcct tccagaaaag ggccccgagg atgcagacgt
    26641 agggcaacat gagggtgcca ttgctattga cccccgcatt ttgcagggcg ttgcccccgg
    26701 cctgcaccag gccgaaggcc gccgccgccc ccagggtggt gcccagcttg ccgtcgccga
    26761 agatcaccgc cgccaccgca atccatcccc ggcccgagct catgccttgg ttaaagagcg
    26821 tgagatagct gatggaaaga aagctccccg ccgcgcccac cagtacgccg ttgaggacca
    26881 gggcaaagta gcgcagccgg agcacggaaa ccccggcggc ggccaaggcc tcttcgtttt
    26941 ctcccgccgc cttgaggcgg agccccagca cggccttccg gtccacccac agcatggcca
    27001 gcaccaaaaa gagcgtcccc cagaccacca gggaaaagtc ccccaccaca cgatcaaggg
    27061 gggcggagag ccaccccagg tccacgtcgg ggaccttctc catgaagggc gacgaaaaat
    27121 accccttcac gtgaaaaatg ctcttgagca ggagcacgga gaggctgtcc gccagaatgt
    27181 tgagggtcat gcccaccacc cagatgttgg ccctcaggtt gacgcaaaag agggcgaaga
    27241 ggaggttgaa ggccacggcg gcggcgacgc cgcagaaaag cccccccacc cagctctcca
    27301 tgaagtggtt tcccatggcg gcgaagaagg cccccaggag catggctccc tcttggctga
    27361 tgttcagaat gcccgtctgt aaggtccaac acccccccag ggccgccagg atcagggggg
    27421 tcatcatgcg gatggaggcg cgaaacagtt cgatcatggc taatcctccc gcaggcgatt
    27481 cgccagcctg ttggccaccg ccatccccgc cgtcacgaag agcacggagg tggtgacgat
    27541 gaggtccacc acttctgcgg ggatacccgt gaggagctcc atctccaggg ccccggagct
    27601 catggcggca tacaccaggg ccgcccccag gacccccaag gggttatacc ccaccatcat
    27661 ggcgaggatc atccccgtcc aggtgtatcc cttggatatc cccaccagga agcgccccgt
    27721 gtctcccatc accagcaggc tccccgcgag ccccgccagc gctccggaaa gggccatggc
    27781 cccgagctgg cgccgcctca cggggatgcc tccggcggcg gcggcccggg ggttgaggcg
    27841 caccatgcgt ccctcgtaac cccaggcggt ccacagcatg agccccgtca ccaggagggc
    27901 ggcccccagg gccacgaaga cgccggtgtg gagcaccgcc aggtcgctga tccgggaaaa
    27961 gcgtacgaag tcctgtaccc ggtcggtcat gcccgccgag acgtttttgc ccgccagggg
    28021 ccccgtggcc aggtacgacg tgaagtactg ggccacggcg ttgaacatga tggtcaccac
    28081 caccacgttc atgcccaggt caagggagag gcggccggga acccacgccc agaagacccc
    28141 cacagatact ccgaaggcca ggaccaggat catggcgggt agggaaggca tggggggaag
    28201 aaagagtccc gccagggccg cccctagggc ccccatgtag agctgtccct cggcgccgat
    28261 gttccaaacc cccgccgcga aggccacagc gatggagagg gccccgagga tgtagggcac
    28321 cgtccgggcc agggtggacc acagggccgg ggcggacccc agggccccga ccaccatggc
    28381 cccgtaggcc tccagggggt tcaccccgtg ggcgaggaag agcgcccccc ccacgcccag
    28441 ggcccccagg aacgaaagga ggatgatcag gggatcccga tggcgaagcc agaaggcatt
    28501 caagaccttc acccccttta gaccgccccg gcgagggtca cggcggcgag gaaggcccga
    28561 tggagctcca ggaagtcctc gtgctccgcc cggagcgtac gcccccccac ccggtgaaag
    28621 ggaagggccc aggaggcctt gtcacactgg gatgtccagg agaaggtgac ctccaactcg
    28681 taggggggac cgggacacca gggcttgaag gccccctcgc gaaagcgggc cagggcctcc
    28741 cgggtggcct gggcgaggcg ttccgtcgtc acctccggcg ccaggaggcg ggcggcgaag
    28801 cgcccgtgtc cttccttaac agggcaggtt accactccgg gaaggaggct ttcggcctcg
    28861 atgcaggcgg ccacgtcgcc ggtgaccagc gccacgggca cccccagagc gccacagaga
    28921 gccgccccca agccgatttc tcccacggcc cggccgttga ggcgcacctc gtgcaccgtg
    28981 gtggagctca tggtgtggtc caaaagggcc cgctcggtgc cccccatggc atggtacgcc
    29041 acaaaaagcg ccacgtccgc ctcggagacc ccttccatca tgcataggat cttcggactc
    29101 ccgctgatca gctccacttc ccgggggagg gctcggccgt caaggtttcg catggcgtcg
    29161 tgggaatcgt tcaccacgat tctttgggcc cccgcctccc gggccgccgt caccacggcc
    29221 caagtgtctt taagctgcat ttctcggccg aactcgtact ccggcttgtt tcgagagact
    29281 tggtcgaagg agaccacccc cgtggccccc tccatgtcac agctgagaaa aactcgcatc
    29341 atccaccctc cctcggctca gcgccccaag gtctccggag agagctcccc ctcccatccc
    29401 cgcaccaagc cggggttctg gcgcaggggg cgccccaatc ccacgaggtc gcagaccctt
    29461 ccttccagga gctcttcggc ggtcttcgcc gaccggatgc ccccggagca gatcacctgg
    29521 ggacgccccc cgcaggcctc ccggatgcgc cgggcgaagg gaccccacca cgcctccccc
    29581 gggccgtcgt agccgcagag gttccccgaa acgtccacca cgtccacccc ctggagggcg
    29641 atctggcggg cgacccacac cgcctcttcc acatccaggc ccgggctctt ctcccccggt
    29701 agggcatccg acactcccag ccgcacggca aggatcgcgt cctttaccac tccccgcagg
    29761 atgtcgacca gatcccgcaa gaggagcgac ctcccatcta ttcccccgcc gtaactgtcg
    29821 ctccgtcggt tggtgacggg gctcagaaac tgccccagga gaaagccgtg gcaggcatgg
    29881 agttccaccc ccgcgaatcc cgccgccagg gcccggcggg cggcggcggc gaagtgctcc
    29941 tctatttgga agatttcttt ttccttcagg tatcgtgggg tcaggcccac ctcatcccgg
    30001 ggatgcctca ccggggaggc ggacaccgcc tcgaagcccg gcgtcagaag ccgggggtct
    30061 ttgatcgcgc tccccgcgtg gttgagctgg gccaccacgg gaactcccgc ctctttgaag
    30121 ggtttcagga aggcgcggta gggctccacc aggtcgtctt ggtccatcat gaattgcccc
    30181 cgacgaacct taccgtcggc ccggaccgcc gcgtgctcca gcaccaccag gcccacgccg
    30241 ctggcggcca ggggagcgta gaactcccga gaccgatccc cgggggctcc gctttcttcg
    30301 tcccccgtcc ccgaggccat gggagccgcc acgaagcggt tacgcagccg tactccccga
    30361 agttccaccg gttcgaaaca ccgccaagat gccacgttca tcgccacctt t
//
LOCUS       DAOOER010000023        29980 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_1768_length_29980_cov_8.858513, whole genome shotgun sequence.
ACCESSION   DAOOER010000023 DAOOER010000000
VERSION     DAOOER010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 29980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29980
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1768_length_29980_cov_8.858513"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            415..1110
                     /locus_tag="PL162_04750"
     CDS             415..1110
                     /locus_tag="PL162_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQF91363.1"
                     /translation="MDEKSPISLKSLREQVYDYLREAMNTGDLVPGSFMNLGEISARL
                     GISKTPLRDALLQLETEGFVRILPRRGCVVQTVTMEDIRDMYQIIGALEASVVLEVGR
                     RLQVSVESMRQLVKEMRFCLGKDDFNGFYEANLRFHNAYLLLSANEPLKRVVQLQKQR
                     LYDFPRRQTFVKEWEVASTGEHDTFVDLVAEGRLEAAASFVRDVHWSFVLQERFIAQY
                     YTDAIRHARERRP"
     gene            complement(1145..1756)
                     /locus_tag="PL162_04755"
     CDS             complement(1145..1756)
                     /locus_tag="PL162_04755"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HQF91364.1"
                     /translation="MTDPCPPAAPGRCPWGELHPLLTAYHDEEWGVPLTDDRRLFEFI
                     VLEGAQAGLSWLTILKKRPGYRRAFHDFDPQAVARMGEEDVERLIQNSAIIRNRRKIA
                     GTLTNARAFLAVQERFGSFARYLWDFCGGIPRQNSWERPEDVPPLTPEAERLSADLKR
                     RGFVFVGATVCYSHMQAVGMVNDHLVSCFRHAAVAALGKNPPL"
     assembly_gap    1792..1891
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1892..2423
                     /locus_tag="PL162_04760"
     CDS             <1892..2423
                     /locus_tag="PL162_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91365.1"
                     /translation="MVTCDDKKLMALALVVLRENEGAPRSDLARIVLEADLLHYEVWK
                     RPLTGGKYDRALVGPVPQGLERVLEGAERGEILRRRRGEDGRERYHPGMHFPIKLAEA
                     QEELTPEERDSLMAALGFVRSLSPEERGRRSRSFAAWRLGSGVLSLEALACPSEEALF
                     SLEDRLWDEGKSREGR"
     gene            complement(2465..4087)
                     /gene="cls"
                     /locus_tag="PL162_04765"
     CDS             complement(2465..4087)
                     /gene="cls"
                     /locus_tag="PL162_04765"
                     /inference="COORDINATES: protein motif:HMM:TIGR04265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HQF91366.1"
                     /translation="MSKNNRRPHSLIGMGALVALGAVGLFLAVQHFDYLHQGVRALGD
                     SLAQGSLWEDGLRLARGVYAALRPYFPHLVTAYALIIAGVVFLEHQNPYRTIAWILAL
                     VAFPVVGVLLYVYLGSSFSQRRTFRRIPRDERQHEDATEALRRLYGVLDCEEALPPAP
                     WRLIPLLEQNSGARLTFRNHVEVLTNGEATFGALMAALEEARESIHLEYFSIAHDELG
                     DAVREILCAQARRGVGVRVLYDAVGSWGMGHDFAKLLKDAGAEVAPFSPVALPMLRRD
                     FNFRNHRKIAVTDGTVGFLGGLNIGDMYLGKSRLGFWRDTHLKIQGEGVGELQKIFLR
                     DWSFATRAKLSPEGLFPPVAEDLPESPLQIVPSGPDSEWNAVEQGYFTLIASAQRRLW
                     LSTPYLVPTDGILSGLISAALGGVDVRLVIPSYPDHRTVYWASKSFLDPLLRAGARVF
                     LYKKGFTHAKVIIADDDTASVGSTNMDLRSLAINFEVQAFIYDRSVVRRLEEDFEVDF
                     ADSHEVTWEEWSRRSLLDRARESGARLLSSQL"
     gene            4509..5300
                     /locus_tag="PL162_04770"
     CDS             4509..5300
                     /locus_tag="PL162_04770"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HQF91367.1"
                     /translation="MARRAPIYIYGTLLLALGMGILLAGTPSGAEAPLATLTVAPGAD
                     VKPGETVSFSVEIPYSDPAPVVAVMVDGAMWSTERQSVTFPGVGRPATVKFTKAYPVP
                     PDAKPGTAICFVLVKGEEKSRKREVISQKHCVMVRDSVTFQPAKKIDPAKFVLMPDLV
                     VTDAHIDPNNGEIVQVKVVNQGQGDAGESVLCVQVRLWSGGGSKQLANRQAKVPPLKA
                     GQELWLSVDTGVLGTFYYEFTADCKNDLKEVNESNNVGYLDRTIK"
     gene            5656..6762
                     /locus_tag="PL162_04775"
     CDS             5656..6762
                     /locus_tag="PL162_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HQF91368.1"
                     /translation="MAEVYFADLRARRRQDSLAAKVRRLVERAGISQVVAEKDLVAVK
                     VHFGERGGDAYVSPVFVRQVVDAVKAQGGKPFVTDTNTLYSGSRANSVDHLTTAVEHG
                     FDFAVLGCPVIIADGLRSGSVVDLPGVGKIFSKVHAAADLVASDALVVLSHFKGHEMA
                     GFGGAVKNLAMGCAPAAGKRDQHALRATVHREKCVGCGECVEVCPVHAINLAEGRALI
                     DRGVCIGCGECMTVCPQRAIDTSNPEDLRDFAERLAEYATALAAPRREKTLFVNFVLR
                     VTPDCDCVPWSDAALVPDQGILASRDPVALDQACFDLVRQAPALPGTLLECACPPGED
                     KFSALWPQTCGLRQLEYAEELGLGSRAYALIPLD"
     assembly_gap    6813..6912
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6913..8003)
                     /locus_tag="PL162_04780"
     CDS             complement(<6913..8003)
                     /locus_tag="PL162_04780"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HQF91369.1"
                     /translation="MRLPPEVLERFVASYFSRRESFLAAPSPAYLLDGQVLRARAITF
                     RRTFQELRRPCSFYFAVKSNNTPRVAAILLEEGFGLDVSGAPELEGALALGARDIIFS
                     GPGKTAEELRLAAAHPEVTVLLDSFGELERLEGEAAARGREVRAGVRLSPEPGALWSK
                     FGIPPEDLPRFCREAQGCPHVRLEGVQCHTSWNMSPLPQCRFLETIAGTLENVDQKSR
                     ELLTFLDVGGGYWPERGEWLRDDAPSPDPLGHRLVPASPLAHYARDLARTLGAHGSLR
                     ALGRLCFEPGRWICDDAMHLTFSVVDVKGPDVAIADIGTNAIGWERFEQDYCPVLNLS
                     RPSLRERPGVILGSLCTPHDVLGFSYFGEG"
     gene            complement(8000..9235)
                     /locus_tag="PL162_04785"
     CDS             complement(8000..9235)
                     /locus_tag="PL162_04785"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HQF91370.1"
                     /translation="MIPRKDPVIVVGTTPDYIDELRRKAPGEAIFLTDRNLRRRAIEE
                     PPGPQEELLGDLASFSAAAALLREHLERWGLRPGGVACYDCEALELAAFLAERFALPF
                     ASPEAVARCRDKFRCKELWRAHRVPCPEARGVATEEELLAFWASLEAPVVLKPRRGTG
                     SELVFRAETPEECRRAFAALHEGVRRCPELGGMVAEEYVAGPEYSCDFLVSAGEVHVL
                     RLTRKMPLAGTFGGTRGYLLCPRPPRWWRRSDFSPLLVRAAAALGLSRAFCMVDFILR
                     RGEPLFLELAPRPGGDCLPPLLRVARGWDPLLAELAFARGRDEKQDPFPGGPGHLGLR
                     LVASRGGVVRRLHTAPLEEDPRVLAVGGLRGPGHRVILPPEDYDSRILGYAIARLEPR
                     NPEIQAQELDALWRVEMAP"
     gene            complement(9232..10164)
                     /locus_tag="PL162_04790"
     CDS             complement(9232..10164)
                     /locus_tag="PL162_04790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQF91371.1"
                     /translation="MGRIRAVDDLQECRELWLRLWPQECLFDLWDVRECFARRLGTTP
                     LFIVAEEGGEPAGLLALSSPAEGEGFVAFPGETWRGETWLEQNRIPSRDPAVTEALIQ
                     SVPGALRLRYLGASSLPPGEGCFDEMNYVFVPEAYGFDFDRYWGSFSPRTRRRIEEET
                     ARLEAHGLAFRHDHAEDLETLFCMSLRAFGEASYFWNSRFLAGFEDLAQTLHRMGMLR
                     VTTVLVGGRVAAVDLGGMFQEGYTLLVGGTDPEFPGVAKVINLYHILRGCRERLRFVD
                     FCSGDFGWKDRFHLERRPLFRIDRPEKHPPEEAP"
     gene            complement(10632..11519)
                     /locus_tag="PL162_04795"
     CDS             complement(10632..11519)
                     /locus_tag="PL162_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQF91372.1"
                     /translation="MTFRPWRCAVLSGKGGTGKTVITAALVRALAGRVLAVDADVDAP
                     NLSLALTVTRDVTHPFFGLPGARVRPEACTGCGACVEACRFSALELRGGKAWGDEGRC
                     EGCGVCAFVCPEKAIDLVPRSQGIFFQGSCPEGPFWGAQLRPGGENSGKLVQCLLEHA
                     AQDARDRRIPLVLIDGPPGTSCPAVSTLGGADAALLVAEPTPTGEEDLGRMGELCRLF
                     AIPAAVVANRSDLTPRGTAELRRRTEGFGGTFLGSIPFLPEIPRLIAQRENPLASLGP
                     HIAPLIDFLHSSMARGEEA"
     gene            complement(11516..12412)
                     /locus_tag="PL162_04800"
     CDS             complement(11516..12412)
                     /locus_tag="PL162_04800"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQF91373.1"
                     /translation="MDRGAGAITARDGRRSPFILAVASGKGGTGKTAVASSLAALLPE
                     VLAVDGDGDAPNLHLLLPFPFRESALITRPRPVVDPNRCDGCGACARACRFGALLTLG
                     GSWRLLGDLCHGCGLCVRVCPRGALREEEYPVGERRRGEEGDREILEGRLFPGLPNPV
                     PVIEDTVAAAPSSTKPAVIIDCPPGSSCPFVTSVRPAHAVLLVTEPTPFGLADLELAL
                     GALKDMGKRVGVLVNRCDVASFAPEELCAREGVPVVGRLPFSRAVAESYAEGVPPALR
                     DHLWRETLTHLWHHWALKGGPA"
     gene            complement(12381..12782)
                     /locus_tag="PL162_04805"
     CDS             complement(12381..12782)
                     /locus_tag="PL162_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HQF91374.1"
                     /translation="MSALRRVAFPVEGGVLCPHFGHAPHFLVVDLEEDREVGRQLLEN
                     PGHTPGSIPRWLQGLQVHTVVAGGIGARAAALFAESGIDLISGVSGPADDAVRAFAEG
                     RLESTGTLCASGHHHHGEGESCGSGGGGHHC"
     gene            13671..15218
                     /locus_tag="PL162_04810"
     CDS             13671..15218
                     /locus_tag="PL162_04810"
                     /inference="COORDINATES: protein motif:HMM:NF013784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HQF91375.1"
                     /translation="MSWSPSLGTPVTHTRLRSPGHVADLSGMCAACTADCPGPCEIGL
                     SALRGAEALLPFAADKNQFASEKRYPLDFSHFNINGRVFGAVGLPEDPYEATYPHAAI
                     ASSFGGAHPVPVRAPFILPAMAKLAWREYFAGAALFGVPAVIGEDVVAKDPGLVVEGH
                     RVVASPLIADMVEAFRRYHRGFGDIVLQANFDDEYHGVLEYAIARLGVTSVELKFGQA
                     AKGIQGMGRVAKREDALRFKALGYVVIPDPTDPAVAEAHARGVGPEFEKIGKLPFWAP
                     EELVARVAELRRLGAQRVCFKTGPFDPRDLVQILEIASEAGVDLVTFDGAGGGTGHSP
                     VKMMNEWGMPTVELGALLWKVLSAWKKRGKACPPVALAGGFVTEDQVYKGLALGAPHV
                     GTIALGRGAMAAASVGRLVGEALKEGTVPPEYARFGSTVEELFADLRVLKQIYGDEAV
                     QIPPGALGVYSYLSRVAVGLQQFMALNRKFALAHIGREDLVPLTERAARATGLLTYEE
                     RLEALMK"
     assembly_gap    15224..15323
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15326..15868)
                     /locus_tag="PL162_04815"
     CDS             complement(15326..15868)
                     /locus_tag="PL162_04815"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HQF91376.1"
                     /translation="MPLTVPPHPAEDPVSRCLEALRRTGCRVTPQRRLIVETLLDTPG
                     EHLNAQELFTRLRRQDPTMGLATVYRTVELLADLGLVHRLRQDEGFARFGLAEGHATV
                     RLVCRCCGHSENAPPEVLSALEKWSQDSSFSLVPQGVAFIGTCLSCQKRREEGGLPRA
                     ICDQCGCGRRRRGCPRREED"
     gene            15969..16406
                     /locus_tag="PL162_04820"
     CDS             15969..16406
                     /locus_tag="PL162_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HQF91377.1"
                     /translation="MATLVAVCVGSVKQEPKEPREEGILDRGWGLLGDAHAGMGRRSV
                     SLLRREDVAKREEEVGFALPPGCLAENLVIDGLPEILPEGTLLRFPSGVVLQVTERGK
                     RPEEPHSYSYRGECLLPTVGYFLTVLEGGTVRPGDEVAWAFPR"
     gene            complement(16410..17318)
                     /locus_tag="PL162_04825"
     CDS             complement(16410..17318)
                     /locus_tag="PL162_04825"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HQF91378.1"
                     /translation="MGLSFVPLSLDLQGAYAARLPLTATRPSMASFVSLWAWRREMEL
                     SWAWEEDLVWVRALRPVPCLLPPVGDWDGVEWGSRLRAHFGPRDETFCSIPSELALRW
                     HRALPGAILREEERGEWDYLHRAEDLKILRGNTFMKKRNKISEFLRDAGQSLEVFPLA
                     PEHLDEVWRFQERWLRSQEDPRGTLAAEHRAIREVFEAWGHLPTVRGTAIRVRGEIAA
                     YAVGALLDPQTLVVHFEKGEGALRGSYQAINQAFVRQHATPAVTWVNREEDLDDPGLR
                     QAKLSYRPVGFVRKERVVLKAPLPPA"
     gene            complement(17326..18975)
                     /locus_tag="PL162_04830"
     CDS             complement(17326..18975)
                     /locus_tag="PL162_04830"
                     /inference="COORDINATES: protein motif:HMM:NF021507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HQF91379.1"
                     /translation="MNLRRLRPLIILPSLMLLLLPAPSSAVSRGEVVAAVVRALGLPP
                     WSGKERFADVPPHHPLASFIETASALGILLPGDHFHPDMESTRAEALGFAFLALGWRK
                     EGELVRALDPSLDPSLPPFLACYYRIAEGMNPTPPRELLSQGRENCSPLDLQRIVAWL
                     KTCRAQRPQWDARYPGTCGDLVVHREGVGTPPAGWSIAVQGLLAESEARALAAQFKKQ
                     GLKAAVGDDGFAFAVRIGPYDHYMKAWKDLARVPMKYNPSIVPSGGGSESLFWVAIAG
                     RPEELRGRILLANALGKYRLPLSRIAAASRVEGAVNAGFFGGKKIIGTLISEGHPVAE
                     AYKNRSAIGWNSRGEVYFGNGAYRRHIEPGPGQASRPEAAPRPRTESPIPPPDPLWIL
                     PSAPGPSPEPEAEPEGGDPHPSFTPPARWELVWADEPMGRMDTVIQAGPMLLQHGVAV
                     NPDEGFSKSTVIMKHPRTFVGYDGDRLWWVVVDGRNPWHSMGLTLEETKRLAANLGLS
                     FALNMDGGGSSTLWWQGVVVNAPSGGTERMIPYGVGFGGGL"
     gene            19245..19664
                     /locus_tag="PL162_04835"
     CDS             19245..19664
                     /locus_tag="PL162_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HQF91380.1"
                     /translation="MIDLSQYVAPGRQMELRKAVEYEDTVGNNSVALSPFLSTGGCLD
                     VFVRMAIEMTDKVLPTGYITVGRHVELTHEAPTMMGQEVFFTVTLDRVEGNRLFFALE
                     ARDGEGPVCVGRYERVAVSSVALMDRAQERCRRAAAQ"
     gene            19822..21495
                     /locus_tag="PL162_04840"
     CDS             19822..21495
                     /locus_tag="PL162_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQF91381.1"
                     /translation="METKMGNTDFGRFDLRRELLVALENRGFDTPTPVQERVLGSDVI
                     SRDAVVQAATGSGKTLAFALPLLNAWQGEGRSPRVLVLAPTRELALQTSRETTHFGAP
                     VGVETASLVGGMEMSPQLRQLRRGAGIVVGTPGRVLDHLRRGSLRLDGVHTVVLDEGD
                     HMLDLGFRDELEAILESCPEERRTWLFSATMPPEVLDLARRFLDDPLRISLVEDVARH
                     EDISHRGCLVPTGRAREGLVNVLLAEQPPRALVFCRTREETSATAAFLRDAGFGASAL
                     HGDMTQRERNDALESLRSGRSPVLVATDVAARGLDVAGISHVIQVGLPGDLEAFVHRS
                     GRTGRAGHAGCNMVLVSPREARQLKALLHTSSVEVSWEHVPDGRDVARARRVARVEEA
                     LGGELPDAEHRTWAEGLLDQGDAVDVVARLLRSLTEREPGGFALKDDLTRELARSSRP
                     MREGDRRGPLGPRRDDGRSRGGAYTLRLARGRDQGWEVGRILRSLCSCLDVDRSEVGS
                     IRLREDHTLVDLSSRAQERLSSARPKLEKLGLLDPSVPGASARRRRAAA"
     gene            21693..>26182
                     /locus_tag="PL162_04845"
     CDS             21693..>26182
                     /locus_tag="PL162_04845"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HQF91382.1"
                     /translation="MRRRILWRAALILCLCLGAAAAWGADDLVVKDFTPQGDIVSLSR
                     LEIVFSAPMVPANRVGKALPKEGAPCTFSPKLAGSWKWKDPTTCVFTPASPLAPATTY
                     TAAVRDNLRDVKQRRVSGPLSYTFATPPLAFVRLTQKDFTQDRRLTLDLHFSLPVNPV
                     RLLGYLELFCGGEAVSYSVSGRESSRTLSLRTSPLPQGKKLTVNISAGLPSTRGDRGL
                     PKDVKKELAPVFVLGVSSAYAQSDYPGRGRISVSTTAPVDVEKAASFITLSPAVPFTV
                     ESGYDGFSVVGDFSPRDRVVLSLKKGLPGAAGGKLGEEYRRAFVFPDVPPSLRFPGAG
                     TYLSPLGDLRVPLEGVNVEKAELAVWRVFPNNVPVALGLQNDDVMDSLSGLVVRETLS
                     LGGKVNEVTRRAVDLRDMASPDRGIFLLTLRDPSWNYWDTPRTVVAVTDLGLTAKIFP
                     RGALVWVNSLKELRPVAGAQVRIFSRSNQLLGEGTTDGDGLLTLSRDRPWPGDLVPHI
                     LTAETADDASYLLVGPELLAGAGYGEDIGGDPYLSEGYEAFVFTSRGVFRPGEAAEVT
                     ALVRQQKFEAPVPFPVEFVMTGPDGRESLRRTAMLSPQGFAEARLPLDMGGRTGSYTV
                     RLRLPGGEGRELGSATFQVEDFAPPRIEVLVQGDKKDLLPQQELRLDVEGRYLFGAPA
                     AELPVEGEVFLGDRTFAPKGWEGFIFGDSEKSFSPYQEFLGNANLDGEGRGALFFTVP
                     PQGTLGRPGLLHFRVGVMEEGGRWSYRTTSLPYDPWPIYLGLEVGDRDASVGTRLRIP
                     VGAVRRDGQPATEVETVMAVISRVVWHSALVEIDGRFRFQTREELVEQERAVVPLSQG
                     RGVLPFLPKDPGCYVVRLEDGSSGSTAAVRLFAYGAGGEGNAMPDRVTLTPLQETYAE
                     GEEARVRVAAPFPGKMLITVETDRVTWSQVVDLKGREAEVSIPVTAAMAPNAYVTAWV
                     LRPVAEDSQSWISHRALGVASFAVDQRPFLLGMSLDAPKEILPGNPVELSVRLAGGPG
                     EVQLALVDEGVLGLTDFATPNPWKFFSRKRALGVQAYDLYDDLLPAENSITPLLHPGG
                     GGDAEAMLRGSLSPVRGRRFKPLALVSPLLSTDAEGRATVSFDVPEFSGKVRIMAVGA
                     AGSRSGAVAENALIAREVVAEATLPRAVAPGDKFVIPVVLFSKGSRDVAVTVTPSANA
                     LLSFEAPSYAATIPAGGQGTSLALPCTVGKGTGLATVTLTTSWPGGESVQTVELPVRP
                     AAPRIVLSGAAAFKGGEKGVLDLPRKWFPGTERGLLVLGSSPRVELAEAVRFLETYPY
                     GCLEQTVSGAWPLLVLPDLVAEVDPELTEAGEIRSRLEGAVRRILTFQNYDGGFLMWP
                     EQSGGGWSWPGIYALHFLVAAQREGIDVPEGPLKEALEGLRRQMTEPFSGEALAEEAL
                     RNKAHRGAYAAYVLALAGEPPLAWMEVLREDRAHLQGVGKILLAGAYALAGERQSARD
                     LL"
     assembly_gap    26183..26282
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <26283..26853
                     /locus_tag="PL162_04850"
     CDS             <26283..26853
                     /locus_tag="PL162_04850"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91383.1"
                     /translation="GEVLAEATLDQGARRAVEKLPAGVTLTCEGEGKVYASWTVEGVP
                     EGKVTPLSQGIVLERAFTDREGAPLPENAPIPQGALLNVVLTVTSERNLSQCILADLL
                     PGGMEIDNPRLGVSQGDRENSAIAHVEPRDDRMLVFIDELRGGEPLTYTYRVRAVSKG
                     TFVLPPASAEGMYDPAVRALVPGGVWRIE"
     gene            26951..29188
                     /gene="pbpC"
                     /locus_tag="PL162_04855"
     CDS             26951..29188
                     /gene="pbpC"
                     /locus_tag="PL162_04855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HQF91384.1"
                     /translation="MERRYGRWRRRALAATLGVAAVGLGLGAFWPPNPEGSVGRTPAS
                     FQVTDRSGELLRAYLAPDDVWCLPLSLDAMGRWIPLIAVEVEDRRFFSHSGVDPWALG
                     RAAWQNLTQGKVVSGASTITSQLVRLSSPTPRPRTWPVKVEEFLGALTLERHLSKETI
                     LESYLNKAPFGGNLRGVEAASRAYFGKRAQFLAPSEAALLVGLLQSPTLFRPDRHPEA
                     ARRRRDHILRLLRSRDLLDDQALALALRDPIPPKLFPFPLRAPHGADLLRATRPDEGV
                     VRSTLEAPLQRTAERLLLEALTPFPGSVAAAAMVVENATGAVRAYVGNARWGGADRGN
                     WVDCGDVPRSPGSALKPFAYLRAFERGLLAPGSMLADTPLGMAGRSPRNFDRKYRGAV
                     SARDALADSLNVPAVRVLRLIRGNDLLALLRRWGFNLLRKDSDHYGDALVLGGCEVTL
                     RELAQAYAALGAGGVFHPLRWEEDAPEMPGVRLCSAASAFLVADILSDLERLPGLVRD
                     KLRGTGLSVAFKTGTSYGLRDAWTVAFSPRFTVAVWFGDTGGTPRDGLGGLLLAAPQA
                     VRLLEEAERGKPRRSFVPPPEVGRRRVCTLSGSPLGEACPASRLEWEIVGVSPGGSCP
                     LHERRGGEVVTLWPPELEGFLRGGVAAWQRAPLAVASPLAHGRYVFLPTPEGAPKIPL
                     VAEGGDRGELFWFVDGVFFARQVPGESLFWPLPAVPGEHRVSVADGSGGAAGVTIVVE
                     TGKIP"
     gene            complement(29217..>29980)
                     /locus_tag="PL162_04860"
     CDS             complement(29217..>29980)
                     /locus_tag="PL162_04860"
                     /inference="COORDINATES: protein motif:HMM:NF022655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LmeA family phospholipid-binding protein"
                     /protein_id="HQF91385.1"
                     /translation="AILILLILLVGGSPGGATEGAHFVGKVPSGGDPGSEMLRVLVEH
                     FVPEEATLVMDAPPDGTHTVRHLFLEVVGASAGGFRVARLRLEPSFVTFSPIDDWASP
                     GGLQVKEIVSAKFDAEITEADLNDFLRKNAFGKGSDEWRDATVDLRPGGLCARARYAS
                     GMVSALVELRTKLQVRGGNQICFADYSFSVNGDAQSEGLIREALEGLQPVVDFRDFIF
                     PVKIAFLEMGDDALRVRTRTSPAPFSGITYTYSAP"
BASE COUNT         4754 a         9443 c        10628 g         4755 t
ORIGIN      
        1 atacgggggg cgagatacgg gggaggcggt atacgagggg cgaagcccgg ggggttagta
       61 tccccggggg gagctcgaga acccgccccg gacggggtgg cgccccgggc ggagagcccg
      121 gagaagggga gtgaggcggg ggcgaaggga gcctcggcga tggcaagaaa ttttatttcc
      181 aggggggatt tctgagataa atctcccctc ggaggggcta aagatcaata aaaatcaacc
      241 tttttgtacc cttttcttcg gggaaaaatt ctccaaagga ggggagataa cgaggagctt
      301 ttcccaaagg gcaaagacat ggtatattag acaacagtgg gaccggcccc ccaaagaagg
      361 ccggagggcg cggcggaggc atcttttagg cacaagaaag cggggctgcg ggacatggat
      421 gaaaagtctc ccatttcgct caagtcgcta cgggagcagg tgtacgacta cctgcgggag
      481 gccatgaaca cgggggacct ggtgccggga tctttcatga acctggggga gatcagcgcc
      541 cggctgggca tcagcaagac ccccttgcgg gatgcgctgc tccagctcga gacggagggc
      601 ttcgtccgca tcctcccccg gcgggggtgc gtggtgcaga ccgtgacgat ggaagacatc
      661 cgggacatgt accagatcat cggggccctg gaggcctcgg tggtgctgga agtggggcgc
      721 cgcctccagg tttccgtgga gtccatgaga cagctagtga aagagatgcg cttttgcctg
      781 ggaaaggacg acttcaacgg cttttacgaa gccaacctcc gcttccacaa cgcctacctt
      841 cttctgtcgg ccaacgaacc gctcaaacgg gtggtgcagc tccaaaagca gcggctctac
      901 gattttcccc ggcggcagac cttcgtcaag gagtgggagg tggcctccac gggggagcac
      961 gacaccttcg tggacctcgt ggcggagggg cgcctggagg cggcggcgtc cttcgtgcgg
     1021 gacgtgcact ggtcctttgt ccttcaggag cgcttcatcg cccagtatta caccgacgcg
     1081 atccgtcacg cccgggaaag gcgcccttag ggaggcgggc catccctccg cccccttccc
     1141 ggcctcacag gggagggttt ttccccaggg cggccacagc ggcgtggcgg aagcaactca
     1201 ccaggtggtc gttcaccatg cccacggcct gcatgtggga gtagcacacc gtggccccta
     1261 cgaagacgaa gccccggcgc ttgaggtccg cgctcaggcg ctccgcctcc ggcgtgaggg
     1321 gggggacgtc ttcgggccgt tcccaggagt tctggcgggg gatccctccg caaaagtccc
     1381 atagatagcg ggcgaaggag ccgaagcgtt cctgcacggc caaaaaggcc cgggcgttgg
     1441 tcagggtccc ggcgattttg cggcgatttc ggatgatggc ggaattttgg atcaggcgct
     1501 ccacgtcctc ttcccccatg cgagccacgg cctgggggtc gaagtcgtgg aaagcccggc
     1561 gatatcccgg gcgttttttc aggatggtga gccagctcaa tcccgcctgg gccccctcga
     1621 ggacgatgaa ttcaaaaagc cttcggtcgt ccgtcagggg gactccccat tcctcgtcgt
     1681 gataggccgt tagcagggga tggagctctc cccaggggca tcgccccggg gcggcggggg
     1741 gacaggggtc ggtcaaggga aacgcctcct cgtgcgggat gggcccgggg gnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngatggtgac gtgtgacgat aagaagttga
     1921 tggctctggc tctggtggtc cttcgggaga acgaaggggc ccccaggagc gacctggccc
     1981 gcatcgtgct ggaggcggat ttgcttcatt acgaggtctg gaagcgcccc ctgacggggg
     2041 gaaagtacga ccgagccctg gtggggcccg tgccccaggg attggagcgg gtgctggaag
     2101 gggccgagag gggggaaatc ctccgccggc gccgggggga ggatgggagg gagcgctatc
     2161 accccggcat gcactttccc atcaagctgg ccgaggccca ggaagagctg acgccggaag
     2221 aacgggactc cctcatggcg gccctgggtt tcgtgcgatc cctctccccc gaggaacggg
     2281 ggcgccggag ccgcagcttt gccgcctggc gcctggggag cggggtccta tccctggagg
     2341 ccctggcctg cccctcggag gaggcgcttt tttccctgga ggaccgcctc tgggacgaag
     2401 gaaagtctcg ggaggggcgg tagaggccgt cccggggccg cccctccccg gggaagatct
     2461 gccgtcatag ttgggaggag aggagccggg cccccgattc ccgggcccgg tccaggaggg
     2521 accgccggga ccactcctcc cacgtcacct cgtgggagtc ggcgaagtcg acctcgaagt
     2581 cctcttccag gcgccgcacc acgctccggt cgtagataaa ggcctggacc tcgaagttta
     2641 tggccaggct ccggagatcc atgttggtgg agcccaccga ggccgtatcg tcgtctgcga
     2701 tgatgacctt ggcgtgggta aagccctttt tgtagagaaa aacccgggcc cccgcccgga
     2761 gcagcgggtc caggaaggat ttggaggccc aatacaccgt gcggtggtcg ggataggagg
     2821 ggatcaccag ccgcacgtcc acccctccca gggcggcgga gatgaggccg gagaggatgc
     2881 cgtcggtggg caccaggtat ggggtggaga gccaaaggcg gcgctgggcc gaggcgatca
     2941 aggtaaagta tccctgttcc accgcattcc attcgctgtc ggggccgctg ggcacgatct
     3001 gaagggggct ctccggcagg tcctccgcca ccggagggaa gagcccctcg ggggacagct
     3061 tcgcccgggt ggcgaagctc cagtcccgca ggaagatctt ctggagttct cccaccccct
     3121 ccccctggat cttgaggtgg gtgtcccgcc agaagcccag gcggctcttg cccaggtaca
     3181 tgtccccgat gtttagcccc cccagaaagc ccacggtgcc gtcggtgacg gcgatcttgc
     3241 gatggtttcg gaagttgaag tcccgccgca gcatggggag ggccacgggg gagaaggggg
     3301 ccacctcggc tcccgcgtcc ttcaggagct tggcgaagtc gtgccccatg ccccaacttc
     3361 ccacggcgtc gtagagcacc cgcaccccca cgccccttcg ggcttgggcg cagaggatct
     3421 cccgcaccgc atcgcccagc tcgtcgtggg cgatggaaaa gtactccagg tggatgctct
     3481 cccgcgcctc ttccagcgcc gccatcaggg ctccgaaggt ggcttctccg ttggtgagga
     3541 cctccacgtg gtttcgaaag gtgaggcggg cgccggagtt ttgctccagg agggggatca
     3601 ggcgccaggg ggccggggga agggcctctt cgcaatccag cacgccgtag agccgccgca
     3661 gggcctccgt ggcatcctcg tgttgtcgtt cgtcccgggg gatgcgacga aaggttcgcc
     3721 gctgggaaaa ggaggacccc aggtagacgt agagtagcac ccccaccacg ggaaaggcca
     3781 ccagggccaa aatccaggcg atggtgcggt agggattttg gtgctccaga aagaccaccc
     3841 cggcgatgat gagggcgtaa gcggtgacca ggtgggggaa atagggacgc agggcggcat
     3901 agaccccccg ggccaggcgg agaccatctt cccagaggga tccttgggcc agggagtccc
     3961 ccagggcccg gaccccctga tgaaggtagt cgaagtgctg caccgccagg aagagcccca
     4021 cggcccccag ggccaccagg gcccccatgc cgatcaagga atggggacga cggttgttct
     4081 tggacatgat cattcctcct caacatttca agcccccgga gccttttgtg aaaaaggtct
     4141 ccgagccccc ctagtgtaac agaccggaag ggatttgagc ccgaagatct ctcccgggcc
     4201 gaaggtcgag gcgacctagg ggggcgaagg ccccggtccg atgggggctc cccagggatg
     4261 atgcggcgaa agaccccttt tgcccccgga aagaatcaaa aggggtggaa aaaaaccggg
     4321 tttgggcata agatggcccc gggggagccg cctcaccccc cggataaacc tcgaagcccg
     4381 gcggctccgg tggaagggag gaatctttag aaaaaggacg aatccgggtc gttttccggc
     4441 aaagcctttg aaggcgtgga cgacgtcacg atgcaaagcc cttggatatc aagaggaggc
     4501 gaaaggcgat ggcacgacgc gcacctattt atatttatgg caccttactt ctggccctgg
     4561 ggatggggat ccttctggcg ggtacgccgt cgggggcgga ggcccccctg gcgaccctca
     4621 cggtggcccc cggagccgac gtcaagcccg gagagacggt gagcttttcc gtggagattc
     4681 cctatagcga ccccgccccc gtggtggccg tcatggtcga cggcgccatg tggagcacgg
     4741 agcgccagag cgtgaccttc cccggagtgg ggcgccccgc cacggtgaaa tttaccaagg
     4801 cctatcccgt gccccccgac gccaagccgg ggacggcgat atgctttgtc ctggtgaagg
     4861 gcgaggaaaa gtctcgaaag agggaggtca tcagccagaa gcactgcgtg atggtcagag
     4921 actccgtgac ctttcagccc gccaagaaaa tcgatcccgc caagttcgtg ctcatgccgg
     4981 acctggtggt gacggatgcc cacatcgacc ccaataacgg cgagatcgtc caggtgaagg
     5041 tggtaaatca ggggcaagga gatgcgggag agagcgtgct ctgcgtccag gtgcgcctct
     5101 ggtccggcgg gggttccaag cagctggcca accgccaggc caaagttccc cccctcaagg
     5161 cgggacagga actctggctc tccgtggaca caggggtgct ggggaccttc tactacgagt
     5221 tcaccgcaga ttgtaaaaac gacctcaagg aggtcaacga gagcaacaac gtcggatatt
     5281 tggatcgcac catcaagtaa tccccgccag taggggagcc ataaaagatc atcccccggc
     5341 ccccagggag agcccccctt cgcgaagggg actccccccg ggggccgggg cttcaacggg
     5401 cggggagcgg gatttttcat caaaatccaa tggcatgggc gccgccttcc cccgggcccc
     5461 ccggaggcgg ggggcgccat cttttgttgg gttccgaggg cctcccccgg ggaggcgcgg
     5521 ggggatcccc ggtcaaaaga tccttggctt aggggcgttt ccgccatccc gggagatttt
     5581 gacggcggag ggattttgtg gtggaatgac caggggaaag gcgttacgaa ggacgatggt
     5641 ggggagggac atgccatggc ggaagtttat tttgcggatc tacgggcccg gcggcgccag
     5701 gatagcctgg ccgccaaggt gcggcgcctg gtggagcggg cggggatatc ccaggtggtg
     5761 gcggaaaagg acctggtggc ggtaaaagtc cactttggcg agcggggggg agacgcctat
     5821 gtgagcccgg tcttcgtgcg ccaggtggtg gatgcggtga aggcccaggg gggcaagccc
     5881 ttcgtcaccg acacgaacac cctctattcc ggcagccggg ccaactcggt ggatcacctg
     5941 acgacggcgg tggagcacgg cttcgatttc gccgtgctgg ggtgtccggt gatcatcgcc
     6001 gacggcctcc ggagcggctc cgtggtggat cttcccgggg tggggaagat cttttccaag
     6061 gtgcatgcgg cggcggacct ggtggcctcc gacgccctgg tggtgctttc tcacttcaag
     6121 gggcacgaga tggcgggctt tggcggcgcg gtgaaaaacc tcgccatggg gtgcgctccg
     6181 gcggcgggga agcgcgatca gcacgcgctg cgcgccacgg tgcaccggga gaagtgcgtg
     6241 gggtgtggcg agtgcgtgga ggtttgcccg gtgcacgcca tcaacctcgc cgaggggcgg
     6301 gccctcatcg accggggggt atgcatcggc tgcggggagt gcatgacggt gtgcccccag
     6361 cgggccatcg acacgtcgaa tccggaggac ctccgggatt tcgccgaacg tctggcggaa
     6421 tacgccacgg ccctggcggc tccccggcgg gagaagactc tctttgtgaa cttcgtgctc
     6481 cgggtgacgc cggactgcga ctgtgtgccc tggagcgacg cggcgctggt gcccgatcag
     6541 gggattttgg cctcccggga ccccgtggcg ctggatcagg cgtgctttga tctggtgcga
     6601 caggcccccg ccctccccgg cacgctactg gagtgtgcct gcccgccggg ggaagacaag
     6661 ttttccgccc tctggcccca gacctgcggc ctccgtcagc ttgaatacgc cgaggagctg
     6721 gggctgggga gccgggccta tgcgctgatt cctttggatt agattccccc ctgaggcttt
     6781 cccccggccg agaaaacctc ggtcgcacga gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnccctcgcc gaagtacgaa aagcccagca cgtcgtgggg ggtgcacagg
     6961 gagccgagga tgacccccgg gcgctcccgg agggaggggc ggctcaggtt cagcacgggg
     7021 cagtagtcct gctcgaagcg ttcccacccg atggcgttgg tgccgatgtc ggcgatggcc
     7081 acatcggggc ccttcacgtc caccacggaa aaggtgaggt gcatggcgtc gtcgcagatc
     7141 cagcgtccgg gctcgaagca gaggcgcccc agggcccgga gggatccgtg ggccccaagg
     7201 gtgcgggcga gatcccgggc gtagtgggcc aggggagagg cggggacaag gcggtgcccc
     7261 aggggatccg gcgagggggc gtcgtcccgg agccattccc cccgttcggg ccagtatcct
     7321 ccgcccacgt caagaaaggt caatagctct cgactctttt ggtccacgtt ttcgagggtt
     7381 ccggcgatgg tttccagaaa gcggcactgg ggaagggggg acatgttcca gctggtgtgg
     7441 cactgcaccc cctccaggcg gacgtggggg cagccttggg cctcccggca gaagcgggga
     7501 aggtcttcgg gggggatgcc gaacttgctc cacagggctc caggttcggg ggacaggcgc
     7561 actccggcgc gcacctcccg cccccgggct gccgcctctc cctcgaggcg ctccagctcg
     7621 ccgaagctgt ccaggagcac ggtgacctcc ggatgggccg ccgcgaggcg gagctcttcg
     7681 gcggtcttgc cggggccgct gaagatgatg tcccgggccc ccagcgccag ggccccttcg
     7741 agctccgggg cgccggagac gtcgaggccg aagccctctt ccagcaggat ggcggccacc
     7801 cggggggtgt tgttgctttt caccgcgaag tagaacgaac aggggcgccg gagctcctga
     7861 aaggtgcggc ggaaggttat agcccgggcc cgcaggacct ggccgtcgag gagataggcg
     7921 ggggaggggg ccgcgaggaa gctctcccgc cgggagaagt acgacgcgac aaagcgttcc
     7981 aaaacctccg gaggaagtct catggcgcca tctccacgcg ccacagggcg tcgagctcct
     8041 gggcctggat ttcggggttc cggggctcca gccgggcgat ggcgtacccc aggatccggg
     8101 agtcgtagtc ctccgggggg aggatcaccc ggtgcccggg cccccggagc ccccccacgg
     8161 cgaggacccg ggggtcttct tcgagggggg cggtgtggag acggcgcacc acccctcccc
     8221 gggacgccac gaggcggagc cccagatggc cgggaccccc cggaaagggg tcttgctttt
     8281 cgtcgcgtcc ccgggcgaag gctagttcgg cgaggagggg atcccatccc cgggcgacgc
     8341 ggaggagggg gggaaggcag tctccgccgg ggcggggggc gagctccaga aagaggggtt
     8401 ctccccggcg gaggatgaag tccaccatgc aaaaggcccg ggagagcccg agggcggcgg
     8461 cggcccgtac caggaggggg gaaaagtcgc tccggcgcca ccaccggggg gggcgggggc
     8521 agagaagata gccccgggtc cctccgaagg ttccggcgag gggcatcttt cgggtgaggc
     8581 gcaggacgtg cacctcgccg gcggagacca aaaagtcgca gctgtactcc ggcccggcga
     8641 cgtattcttc ggccaccatg cccccgagtt ccgggcagcg gcgcacgccc tcgtggaggg
     8701 ccgcgaaggc ccgacggcat tcctcggggg tctccgcgcg aaagacgagc tcgctccccg
     8761 tgccccggcg gggcttgagc accaccgggg cttccaggga ggcccaaaaa gccaggagct
     8821 cttcttccgt ggcgacgccc cgggcctcgg ggcagggaac gcggtgggcc cgccagagct
     8881 ctttgcagcg gaacttgtcc cggcagcgtg ccaccgcttc gggggaggcg aagggcaggg
     8941 cgaacctttc ggcgaggaag gcggcgagct ccagggcctc gcagtcgtag caggccactc
     9001 ctccggggcg tagaccccat cgctccaggt gctcccggag gagggcggcg gcggcggaaa
     9061 aggacgccaa atcccccagg agctcctctt gggggcccgg gggctcttcg atggcccggc
     9121 gccgcaggtt ccggtcggtg agaaagatcg cctcgccggg ggccttgcgg cggagctcgt
     9181 cgatgtagtc gggggtcgtt cccacgacga tcacggggtc cttccggggg atcacggcgc
     9241 ttcctccgga gggtgctttt ccgggcgatc gatccggaag agggggcgtc gctccaggtg
     9301 gaagcgatct ttccacccga agtctccgct gcagaaatcc acgaaccgga ggcgctctcg
     9361 gcatccccgg aggatgtggt aaagattgat gactttggcc accccgggga attcggggtc
     9421 ggtgcccccc accaggaggg tgtatccttc ctgaaacatt cctcccagat ccaccgccgc
     9481 cacgcgtccc cccacgagga cggtggtcac ccggagcatc cccatccgat gaagcgtttg
     9541 agccaggtct tcgaagcccg ccaagaagcg ggaattccaa aagtacgacg cctcgccgaa
     9601 ggcccggagg ctcatgcaaa agagcgtttc cagatcttcc gcgtggtcat ggcgaaaggc
     9661 caggccgtgg gcttccagcc gggccgtctc ttcctcgatg cggcgccggg tccgggggga
     9721 aaaggagccc cagtaccggt cgaaatcgaa gccgtaggcc tccggcacaa agacataatt
     9781 catctcgtcg aaacaccctt cccccggggg aaggctcgac gcccccagat aacggaggcg
     9841 tagcgccccc ggcacgctct ggatcagggc ctccgtcacc gccgggtctc tggaggggat
     9901 gcggttttgt tcgagccagg tttccccccg ccaggtctct ccgggaaagg ccacgaagcc
     9961 ttccccttcc gccggagagc ttagcgccag gagtccggcg ggctccccac cctcctccgc
    10021 cacgatgaaa agaggggtgg tgccgaggcg ccgggcaaag cactcccgca cgtcccagag
    10081 atcaaaaaga cattcctggg gccacaggcg gagccagagc tcccggcact cctgaaggtc
    10141 atccacggct cggatgcgtc ccatggggac ccaccgcctc ccgtcgttct agggtttcgc
    10201 ccgaaaaagg gcgggggtgc caaggtattt ccgtggagcc ggtatttctg ccgatggggg
    10261 gccacgacac agggaaaatc tttccctccg gcggggatga gaatcctccg gggcgagagg
    10321 gcgggggcca ggagcgccct gtatcgggcg ccccgggtcc tcatgttcgg ggatttgacc
    10381 gacaaagtca agggatggac gacgccagta ggcttcgcaa agccagagct gccttttttc
    10441 ccgtatacag cagctttcgc catggcaagg acaagatggc ggcatggcgg cgccgaaagg
    10501 agggcgcgac atatttcttg accgggggcc ggctcccggg aaaatgcgtg tgccgtgccc
    10561 ggcggccctc gctacgaagg cccccccggc aaggcctgcg cgccttgccg gggggcgggg
    10621 cgtcttgggg cctaggcctc ttcgcccctc gccatggagg agtgtaagaa atcgatgagg
    10681 ggagcgatgt ggggccccag ggaagccagg ggattttccc gttgggcgat gaggcggggg
    10741 atctccggca gaaaggggat gctccccagg aaggtccccc cgaagccctc ggtgcgccgc
    10801 ctcagctccg ccgtcccccg gggggtgaga tcggatcggt tggccaccac ggcggcgggg
    10861 atggcaaaaa ggcgacagag ctcccccatg cgccccaggt cttcctcccc cgtgggggtg
    10921 ggctcggcca ccaggagggc agcgtcggcc ccccccagcg tggacaccgc cgggcacgat
    10981 gtccccgggg ggccgtcgat gagcaccagg gggatgcggc gatcccgggc gtcctgggcg
    11041 gcgtgttcca gaaggcactg gaccaatttc cccgagtttt ccccaccggg gcgcagctgg
    11101 gccccccaaa agggtccctc ggggcagctc ccctggaaga agatcccctg ggaccggggg
    11161 acgagatcta tggccttttc cggacagacg aaggcgcaga ccccgcaccc ctcgcagcgc
    11221 ccctcgtccc cccaggcctt tcccccccgg agctccaggg cggaaaagcg acaggcctcc
    11281 acgcacgctc cgcaccccgt gcaggcctcg gggcgcaccc gggccccggg aaggccgaag
    11341 aaggggtggg tgacgtcccg ggtcacggtc agggccaggg agagattggg ggcgtccaca
    11401 tccgcgtcca cggccaggac ccgccccgcc agggcccgga ccagggccgc cgtgatgacg
    11461 gtctttcccg tgcccccctt gccgctcaac accgcacatc gccacggacg aaaggtcatg
    11521 cggggcctcc tttcagggcc cagtggtgcc aaagatgggt cagggtctcc cgccagaggt
    11581 gatcccggag cgccgggggt accccttcgg cgtaggactc cgccacggcc cgggagaagg
    11641 gaaggcgccc caccacgggg accccctccc gggcgcagag ctcttccggg gcgaaagacg
    11701 ccacgtcgca gcggttcacc agcacgccga cgcgctttcc catgtccttt agggccccca
    11761 gggcgagctc caggtcggcc agcccgaagg gggtgggttc cgtcaccagg agcaccgcgt
    11821 gggcggggcg gacggaggtc acgaagggac aggaggaccc cggggggcag tcgatgatca
    11881 cggcgggctt cgtcgaagag ggggccgccg ccacggtgtc ttcgatcacc ggcacggggt
    11941 taggcagccc gggaaagagg cgtccctcca ggatctcccg atccccctct tcgccgcgcc
    12001 gccgttcccc cacggggtac tcctcttccc ggagggcccc ccgggggcag acccggacgc
    12061 agaggccaca gccgtggcag aggtccccca gcagacgcca ggagcccccc aaggtgagga
    12121 gggccccaaa acgacaggcc cgggcgcagg cgccgcaccc gtcgcaccgg ttgggatcca
    12181 ccacggggcg gggacgggtg atcagggcgc tctcgcggaa gggaaaggga agcaggaggt
    12241 ggaggttggg ggcgtcccca tctccatcca cggcgagcac ctcggggagc aaggcggcca
    12301 gggaggaggc cacggcggtc ttgcccgtgc cccccttgcc gctggccacc gccaggataa
    12361 aaggagatct tctcccgtcc ctagcagtga tggcccccgc cccccgatcc acacgactcc
    12421 ccttctccgt ggtggtggtg gccggaggcg cagagggtcc cggtgctttc cagacgcccc
    12481 tcggcgaagg cccgcaccgc atcatcggcg ggccccgaga ccccggagat caagtcgatg
    12541 ccgctttcgg cgaagagggc cgccgcccgg gccccgatgc cccccgccac cacggtatgg
    12601 acctggagcc cctggagcca tcgggggatg gaccccggag tgtgcccggg gttttccagg
    12661 agctggcgcc ccacttctcg atcttcttcc aggtcgacca cgagaaaatg gggggcgtgg
    12721 ccgaagtggg ggcacaggac gcccccttcc acgggaaagg ccacccggcg aagggcgctc
    12781 atcgggatcc cgtcccttct cccagggcat cgcgctgggc ccggagctct ttgagctcct
    12841 cttccaggta caggatgcgc ccctcaagca agggcaccgt gtcgggcctc cggcagcacc
    12901 ctctgccgag gcccccgggg ccgccccatc cccgccccat gccgccaaac ccgaacctac
    12961 aacccatgcc gcccatgccg ccaaaccctc tcccctggtg ccaggggccc gtaccgtctc
    13021 cgcgaggcat gtgacatccc tccttattga gatgcgttct caatagctat tctacctccc
    13081 ctcgggggcg gtgtcaaggg atgccctccc cccagatccg cgcacctttg ggaatgctga
    13141 aagcttctct gggccatgcg gaacacggcg cctgagggag gtgccccaag cccccttcct
    13201 tcgccggaag aggggcgatc atggtgcccc agaacgagaa aaaagcccta gggaaaacgg
    13261 cgaaacggcg aaagggcgga agaaaacgcc gcgctcgagg gtacgcatcg ggacacgaga
    13321 cggccccagc ggcatatcgc cccctggtcc cggggggtgg ggcgatccgg ggggggaagt
    13381 cgtctttggt tgatttttcc ctccctcctt ggatgggagg aaggaggcct ccgaaggggg
    13441 ggcacgccag tcctccgagg gcgctatctc ccgttctctc gggggttttc ccctctagaa
    13501 ccgacgaaag ggggtctttt gttgcggggc tttccctgtg ctaccatggg gagcgagagc
    13561 tctttttccg ggggtccttt gcgccgggga tccccgtacc gcgaggaggg ggcgcgaaag
    13621 ccccgggggg ccttcctcgg gggatcataa ttcttgtgga ggtgtgtcga atgagttgga
    13681 gccccagcct tggcaccccg gtgacccata cccggctgcg aagccccggc catgtggccg
    13741 atctttccgg catgtgcgcc gcctgtaccg ccgattgtcc cggcccctgt gagatagggc
    13801 tttccgccct ccgaggggcg gaggccctcc tcccctttgc tgcggataag aatcagtttg
    13861 cctcggagaa gcgctacccc ctggacttct cccacttcaa catcaacggc cgggtcttcg
    13921 gcgccgtggg gctccccgag gatccctacg aggccaccta cccccacgcg gccatcgcct
    13981 cttccttcgg tggggcgcac cccgtcccgg tccgggcccc cttcattctc cccgccatgg
    14041 ccaagctggc ctggcgggag tacttcgccg gggcggccct cttcggcgtc cccgccgtca
    14101 tcggcgagga cgtggtggcc aaggatccgg gcctcgtggt ggaagggcac cgggtggtgg
    14161 cgtcccccct catcgccgat atggtggaag ccttccggcg ctaccaccgg ggctttgggg
    14221 acatcgtgct ccaggccaac ttcgacgacg agtaccacgg ggtgctggag tacgccatcg
    14281 cccgcctggg agtgacctcg gtggaactca agttcggcca ggccgccaag gggatccagg
    14341 ggatgggaag ggtggcgaag cgggaagacg cccttcgctt caaggccctg ggatacgtgg
    14401 tgatccccga tcccaccgac ccggcggtgg ccgaggccca tgcccggggg gtggggcccg
    14461 agttcgagaa gatcggcaag ctccccttct gggcccccga ggaattggtc gcccgggtgg
    14521 ccgagctccg gcgcctgggg gcccagcggg tctgcttcaa gacggggccc ttcgatcccc
    14581 gggacctcgt ccaaatcttg gaaatcgcct cggaggcggg ggtggacctg gtgaccttcg
    14641 acggggcggg gggagggacg gggcacagcc ccgtgaagat gatgaacgaa tggggcatgc
    14701 ccacggtgga gttgggggcg ctgctgtgga aggttctcag cgcgtggaag aagcggggga
    14761 aggcttgccc ccccgtggcc ctggcggggg gcttcgtcac cgaagaccag gtctacaagg
    14821 gtttggccct gggggctccc cacgtgggga ccatcgccct ggggcggggg gccatggcgg
    14881 cggcctccgt gggacgcctc gtgggggagg ccctgaaaga gggcacggtg cccccggagt
    14941 acgcccgctt cggctccacg gtggaggagc tcttcgccga cctccgggta ctgaagcaga
    15001 tctacggaga cgaggcggtg cagattcccc cgggcgccct gggggtctac tcgtacctga
    15061 gccgggtcgc cgtggggctc cagcagttca tggcgctgaa ccgcaagttt gccctggcgc
    15121 acatcggccg agaagacctc gtgcccctca cggagcgcgc ggcccgggcg acggggcttt
    15181 tgacctacga ggagcgcctc gaagccctca tgaaatgagg cgannnnnnn nnnnnnnnnn
    15241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15301 nnnnnnnnnn nnnnnnnnnn nnngatcagt cctcctcgcg ccgggggcac ccgcgccgcc
    15361 ggcggccgca gccgcattgg tcgcagatgg cccggggcaa gcccccctct tctcgccttt
    15421 tctggcagga gaggcaggtg ccgatgaagg ccaccccttg gggcaccagg gaaaaggagg
    15481 agtcctggga ccacttttcc agggcgctta gcacctcggg aggggcgttt tcggagtggc
    15541 cacagcatcg gcacacgagg cgcaccgtgg cgtgcccctc ggcgaggccg aagcgcgcga
    15601 agccctcgtc ttgacggagg cggtgcacca gccccaggtc ggccaggagc tccacggtgc
    15661 ggtacaccgt ggcgagcccc atggtgggat cctggcggcg gagacgggta aagagctcct
    15721 gggcgttcag gtgctccccc ggggtgtcta ggagggtctc gacgatgagg cgccgctggg
    15781 gggtcacccg gcaccccgtg cggcgaagcg cctccaggca ccgcgacacg ggatcttcgg
    15841 cggggtgggg ggggacggtc aagggcatgg cactcttcct ttcgtgggtg gggctctttc
    15901 caggcccatt gtaccccggg aggggccctt tggggctcgg agccaaaaaa catgaggggg
    15961 aatcgacgat ggccaccctg gtggcggtgt gcgtaggatc ggtgaagcaa gagcccaagg
    16021 agccccggga ggagggcatc ctggatcggg ggtgggggct tttgggagac gcccacgcgg
    16081 ggatgggaag gcgctccgtg agcctcttgc ggcgggaaga cgtggcgaag cgggaagaag
    16141 aggtgggttt tgcccttccc ccggggtgtc tggcggaaaa cctggtgatc gatgggttgc
    16201 cggagatcct tcccgagggg acccttctcc ggttcccctc gggggtcgtg ctccaggtga
    16261 ccgagcgggg caaaaggcct gaggagcccc acagttacag ctaccggggg gagtgtctcc
    16321 tgcccacggt ggggtacttt ctgaccgtcc tcgagggggg aaccgtgagg ccgggggacg
    16381 aagtggcctg ggcctttccc cggtgaaccc taggccgggg ggagcggggc cttgaggacg
    16441 acccgctcct tccggacgaa ccccacgggg cggtaggaga gcttggcctg gcgtagcccc
    16501 ggatcgtcca ggtcctcttc ccggttcacc caggtcacgg cgggggtggc gtgctggcgg
    16561 acgaaggcct ggttgatggc ctggtagctc ccccgcaggg ctccctctcc cttttcgaag
    16621 tgcaccacca gggtttgagg atctagcagg gctcccacgg cgtaggccgc gatctccccc
    16681 cggacccgga tcgccgtccc ccggacggtg gggaggtgcc cccaggcctc gaagacctct
    16741 cggatggccc ggtgctccgc cgccaaggtg ccccggggat cctcctggga ccggagccag
    16801 cgctcctgga agcgccagac ctcgtccaga tgctccgggg cgaggggaaa gacttccagg
    16861 ctttgtcccg cgtcccggag gaactccgat atcttgtttc ttttcttcat gaaggtgttg
    16921 ccccggagga ttttcaggtc ttcggcccga tggaggtagt cccactcccc ccgctcctcc
    16981 tcccggagga tcgccccggg gagggcccgg tgccaccgca gggccagctc cgaggggatg
    17041 ctgcagaagg tctcgtcccg ggggccgaag tgggcccgga ggcgcgatcc ccactccact
    17101 ccgtcccagt cccccacggg gggcaggagg caggggaccg gccggagggc ccggacccac
    17161 accagatcct cctcccaagc ccaggagagc tccatttcgc ggcgccaggc ccacagggag
    17221 acgaagctag ccatggacgg gcgcgtggcg gtgaggggca gtcgggcggc gtaggccccc
    17281 tggaggtcca aagacagggg gacgaaggaa agccccatgg tgggcctaga gccccccccc
    17341 gaagcccacg ccgtagggga tcatgcgctc cgtccccccc gagggggcgt tgaccaccac
    17401 gccctgccac cacagggtgc tggatccccc tccatccatg ttgagggcga aagaaagccc
    17461 caggttcgcc gccaggcgct tggtttcctc caaggtgagc cccatgctgt gccagggatt
    17521 tctcccgtcc accaccaccc accagagccg gtccccgtcg taacccacga aggtgcgagg
    17581 atgtttcatg atgacggtgc tcttcgagaa tccctcgtcg gggttcaccg ccacgccgtg
    17641 ctggaggagc atggggcccg cctggatgac ggtgtccatg cgccccatgg gctcgtcggc
    17701 ccagaccagc tcccaccggg ccgggggggt gaaagagggg tggggatcgc ccccttcggg
    17761 ctcggcctcc ggctcggggg aggggcccgg ggccgaggga aggatccaca ggggatcggg
    17821 gggagggatg gggctttccg tccggggacg gggggcggcc tccggcctgg aggcctgtcc
    17881 cggtcccggc tcgatgtggc gccgataggc gccgttgccg aagtagacct cgccccgaga
    17941 gttccatccg atggcggagc ggttcttgta ggcctcggcc accgggtgtc cttcggagat
    18001 cagggtgccg atgatctttt tccccccgaa aaagcccgcg ttcaccgccc cctccacccg
    18061 agaggcggcg gcgatgcggg acaggggaag gcggtacttt cccagggcgt tggccaggag
    18121 gatgcgcccc cggagctcct cggggcgacc ggcgatggcg acccagaaga ggctctcgga
    18181 gccccctccg gaggggacga tgctggggtt gtacttcatg gggacccggg ccaggtcctt
    18241 ccaggccttc atgtagtggt cgtaggggcc gatgcgcacg gcgaaggcga agccatcatc
    18301 ccccacggcg gcctttaatc cctgtttctt gaattgggcc gccagggcgc gggcttcgct
    18361 ctccgccagg agcccctgga cggcgatgga ccaccccgcc ggaggggtcc ccactccctc
    18421 ccggtggacc accagatctc cgcacgtccc gggatagcgg gcgtcccact ggggacgctg
    18481 ggcgcggcag gttttcagcc aggcgacgat gcgctggagg tccagggggg agcaattctc
    18541 tcgcccttgg gagaggagtt ctcgaggagg ggtggggttc atgccctcgg cgatgcggta
    18601 gtagcaggcc agaaaggggg gaagggaggg gtccagggag gggtccaggg ccctcaccag
    18661 ctctccttct ttgcgccatc ccagggccag aaaggcaaag cccagggcct ccgcccgggt
    18721 ggattccatg tccgggtgga agtgatctcc ggggaggagg atgcccaagg ccgaggccgt
    18781 ttcgatgaaa gacgccaggg ggtgatgggg ggggacgtcg gcgaagcgct ccttgccgct
    18841 ccagggggga aggcccaggg cccgcaccac ggcggccacc acctctcccc gggagacggc
    18901 ggaagatggg gcgggaagga ggagcaacat cagggacggg aggatgatca gggggcgaag
    18961 tcttcggagg ttcacgggtt cgggctcctt tgcgatgcaa aatttgcctt tgggttcgaa
    19021 aaaggcacgg gatcgcagat gttatacacc atggagggcc caagggccat ccgccgaaag
    19081 actcaccccc cgtcggaggc ggagatcccc ttgttttcgg cgaggggccc gggggtcgaa
    19141 gtggggggag acgcgccctt ttagaacgcg ttataatggg ggccgcctcc cgtcggagat
    19201 tccttcggag gaaacgccat tattttttta ggaggggacc gacgatgatt gacctttccc
    19261 agtacgtggc tccggggcgt cagatggaac ttcgcaaggc cgtggagtac gaggacacgg
    19321 tgggcaacaa ctccgtggcc ctgagcccgt ttctctccac ggggggatgc ctggacgtct
    19381 tcgtccgcat ggccatcgag atgacggaca aggtgcttcc cacgggttac atcaccgtgg
    19441 ggcgccatgt ggagctcacc cacgaggccc ccaccatgat gggtcaggag gtcttcttca
    19501 cggtgacctt ggatcgcgtg gagggaaacc gcctgttttt cgccctggag gcccgggatg
    19561 gggaggggcc ggtctgcgtg gggcgttacg aacgggtggc ggtgagcagc gtggccctca
    19621 tggaccgggc ccaggagcgc tgccgccgcg ccgccgccca gtagcccggg gcctgcggtt
    19681 tggaccttcg ggggttgaca tttatcggta taggaggata atgacccctg gccgtcggaa
    19741 cgggaagttt tttgggtgcg tgaccgagca agatggcggt cacgtttttt ttgccccgag
    19801 acggggcagg gaggttcata cttggagacg aagatgggaa acacggattt tgggaggttc
    19861 gacctgcggc gagagctcct cgtcgcgctg gagaaccggg gttttgacac tcccacgccg
    19921 gtgcaggagc gggtgctggg atcggacgtg atatcgaggg atgcggtggt gcaggccgcc
    19981 acggggtcgg gcaagaccct ggcgtttgcg ctgccgcttt tgaatgcctg gcagggagag
    20041 gggcgttccc cccgggtgct ggtgctggcc cccacgaggg agttggcgct ccagacctct
    20101 cgggagacca cgcacttcgg cgcccccgta ggggtggaga cggcctccct ggtgggagga
    20161 atggaaatgt ccccccagct tcgccagctc cgtcgcgggg cgggcatcgt ggtgggtacc
    20221 ccggggaggg tgctggatca cctgcgccgg ggatcgttgc ggctcgacgg ggtgcacacc
    20281 gtggtcctcg acgagggaga tcacatgctg gatctgggct tccgggacga gctggaagcc
    20341 atcttggaga gctgtccgga ggagcggcgc acctggctct tctccgccac catgcccccc
    20401 gaggtgcttg acctggcccg gcgctttttg gacgaccccc ttcgcatttc cctggtggag
    20461 gacgtggcgc gccatgagga tatctcccac cggggatgcc tggtgcccac gggacgcgcc
    20521 cgggaggggt tggtgaacgt ccttttggcg gaacaacccc cgagggcgct ggtgttttgc
    20581 cgtacccggg aggagacctc cgccacggcg gctttcctgc gggatgcggg cttcggggcc
    20641 tcggcgctcc acggagacat gacccagcgg gaacggaatg acgcgctgga atccctgcgc
    20701 tcgggacgat ctcccgtgct ggtggccacg gacgtggcgg cccgggggct ggacgtggcg
    20761 gggatctccc acgtcatcca agtgggactc cccggcgacc tggaggcctt cgtgcatcgc
    20821 agtgggcgca cggggcgggc ggggcacgcg gggtgcaaca tggtgctggt ctccccccgg
    20881 gaggcccggc agctcaaggc cctgctccac acctcctcgg tggaggtgtc gtgggaacac
    20941 gtccccgacg gtcgggacgt ggcccgggcc cggcgggtcg cccgggtgga agaggctctg
    21001 gggggcgagc tccccgacgc ggagcaccgc acctgggccg aaggcctcct ggaccaaggg
    21061 gacgcagtgg acgtggtggc ccggctcctg cgctccctga cggagcgcga gcccgggggc
    21121 tttgccctga aagacgacct tacccgggag ctggcccgat cctctcgccc catgcgagaa
    21181 ggggaccggc ggggccctct gggacctcgg cgggacgatg gacgatctcg ggggggcgcc
    21241 tatacccttc gtctggcccg gggacgggat cagggatggg aagtggggcg catcctccgg
    21301 agcctctgct cctgtctgga cgtggaccgc agcgaggtgg gatccatccg cctgcgggag
    21361 gaccacaccc tggtggacct ctcctcccgg gcccaggagc gcctctcttc ggcccgcccc
    21421 aaactggaaa agctcggttt gctcgatcct tccgtccccg gggcctctgc ccggcgccgg
    21481 agggcggcgg cgtagggatc tccttagggc gagaaaaagg cttgaaatag gcgacggcta
    21541 tggcgtgggg gccccggggc ccccacgttt tttctttggc ggggcgaagg aggaaggccc
    21601 ggggcctggg gaaagttttc ccctgggata gtgctataat gcgccttggg gcgcccatgg
    21661 ggcgctttga aacttcagag gagggatggt agatgcgacg acggatcttg tggcgtgcgg
    21721 ccttgatttt atgcctctgc cttggggcag cggcggcctg gggggccgac gatctggtgg
    21781 tgaaagactt caccccccag ggggatattg tttccctttc ccggttggag atcgtctttt
    21841 cggcccccat ggttccggcg aaccgggtag gcaaggccct tcccaaggag ggagccccct
    21901 gcaccttttc gccgaagctg gcgggatcct ggaagtggaa ggaccccacc acctgcgtct
    21961 tcacccccgc ctcccccctc gcccccgcca ccacctacac ggcggcggtg cgggacaatt
    22021 tgcgggatgt caagcagcgg cgcgtctcgg gccccctgag ctacaccttc gccactcctc
    22081 cccttgcctt cgtgcgcctg acccaaaagg actttaccca ggaccggagg ctgactttag
    22141 acctccactt ctccctgccg gtgaacccgg tgcgccttct gggatacctg gagctcttct
    22201 gcggcggcga agcggtgtct tattccgtct ccgggcggga gtcctcccgg accctgtcgc
    22261 tgcgcacatc cccccttcct caggggaaaa agctcacggt gaatatctcg gcggggctgc
    22321 cctccacccg gggagatcgg ggccttccca aggacgtgaa aaaagagctg gccccggtct
    22381 tcgtcctggg ggtgagcagc gcctacgccc aatccgacta ccccgggcgg gggcggatct
    22441 cggtgagcac cacggccccc gtggacgtgg aaaaggccgc ctctttcatc accctctcgc
    22501 cggcggtgcc ctttacggtg gagtccggct atgacggctt ctccgtggtg ggggacttct
    22561 cccctcggga ccgggtggtg ctgtctttga aaaagggcct ccccggcgcg gcggggggca
    22621 agctgggaga agaataccgt cgggccttcg tcttccccga cgttccccca tcgctgcgct
    22681 ttcccggcgc ggggacctac ctgtcccctt tgggagatct gcgggtgccc ctggaagggg
    22741 tgaacgtgga aaaggccgag ctggcggtgt ggcgggtctt tcccaacaac gtccccgtgg
    22801 ccctggggct ccagaatgac gatgtcatgg acagcctctc cggcctggtg gtccgggaga
    22861 ccctgagcct cggcggcaag gtgaacgagg tgacccgaag ggccgtcgac ctgcgggaca
    22921 tggcgagccc cgaccggggg atcttcctcc tgacactgcg agatccctcg tggaactact
    22981 gggacacccc ccgcaccgtg gtggcggtga ccgacctggg actgacggcc aagatcttcc
    23041 cccggggagc cctggtgtgg gtcaactcct tgaaggagct gcgccccgtg gcgggggccc
    23101 aggtccggat cttctcccgg tcgaaccagc tcctgggaga ggggaccacg gacggagacg
    23161 gacttttgac cctatcccgg gatcgcccct ggcccggaga cctggtgccc cacattctca
    23221 ccgccgagac ggcggatgac gcctcctacc tcctggtggg ccccgagctc ctggccggag
    23281 cggggtacgg cgaagatatc gggggagatc cctacctgtc cgagggatat gaggccttcg
    23341 tcttcacctc ccggggggtc tttcgccccg gcgaggcggc cgaagtcacc gccctggtgc
    23401 gccagcagaa gttcgaagct ccggtgccct ttcccgtgga gttcgtcatg acgggccccg
    23461 atggccggga atccttgcgc cgcacggcga tgctctctcc ccagggcttt gcggaggccc
    23521 gactccccct ggacatgggg gggcggacgg ggtcctacac ggtccggctt cgcctccccg
    23581 gaggcgaggg gcgggagctg gggagcgcca ccttccaggt ggaggacttt gcccctcccc
    23641 gcatcgaagt gttggtgcag ggggacaaaa aggacctcct gccccagcag gagctgcgcc
    23701 tggacgtgga ggggcgttac ctcttcggcg ccccggcggc ggagctcccc gtcgagggag
    23761 aggtcttcct gggggatcgg acctttgccc ccaagggatg ggagggcttc atcttcggcg
    23821 actcggagaa gtccttttcc ccctatcagg agtttttggg caacgccaat ttggacggag
    23881 aggggcgggg ggccctcttt ttcaccgtgc ctccccaggg caccctgggg cgtccgggct
    23941 tgctccactt ccgcgtaggg gtcatggagg agggggggcg gtggtcctac cggacgacct
    24001 ccctgcccta cgacccctgg cccatctacc tcggcctgga ggtgggggat agggacgcct
    24061 ccgtggggac ccggctccgg atccccgtgg gggcggtgcg ccgagatggc caacccgcca
    24121 ccgaggtgga gacggtgatg gcggtgatct cccgggtggt gtggcacagc gccctggtgg
    24181 aaattgacgg ccgattccgg ttccagaccc gggaggagct ggtggaacag gagcgcgccg
    24241 tggtgcccct ttcccagggc cggggagtgc tccccttcct gcctaaagac ccggggtgct
    24301 acgtggtgcg cctggaagac gggagttcgg gaagtaccgc cgcagtgcgc ctcttcgcct
    24361 acggcgccgg gggcgagggc aacgccatgc cggaccgggt gaccctcacc cccctgcagg
    24421 agacctacgc cgaaggggaa gaggcccggg tgcgggtcgc cgcccccttc ccggggaaga
    24481 tgctgatcac ggtggaaacc gaccgggtga cgtggagcca agtggtggat ctgaaggggc
    24541 gggaggcgga ggtttccatc cccgtgacgg cggccatggc ccccaacgcc tacgtcacgg
    24601 catgggtgct ccgccccgtg gcggaagaca gccagtcgtg gatcagccat agggccctgg
    24661 gagtggcgtc tttcgcggtg gatcagcggc cgtttttact ggggatgtcc ctggacgcgc
    24721 cgaaggaaat cctgccggga aatccggtgg agctttcggt gcgcctggcg ggggggccgg
    24781 gggaagtcca actggccctg gtggatgagg gcgtcctggg gctcacggat ttcgccactc
    24841 cgaatccctg gaagttcttc tctcggaaga gggccctggg agtccaggcc tacgatctct
    24901 acgacgacct ccttccggcg gaaaatagca tcacccccct gctgcacccc ggagggggcg
    24961 gggacgcgga ggccatgctg aggggatccc tatcccccgt acgggggcgg cggtttaagc
    25021 ccctggccct ggtgagcccc ctgctgtcca ccgacgccga ggggcgcgcc acggtgtcct
    25081 ttgacgtgcc ggagttcagc ggcaaggtgc ggatcatggc ggtgggggcg gcgggatcgc
    25141 gcagcggcgc ggtggcggaa aacgccctga tagcccggga ggtggtggcg gaagccacgc
    25201 tgcctcgggc ggtggcgcca ggggataagt tcgtgatccc cgtggtgctt ttctccaagg
    25261 ggtcgcggga cgtggcggtg acggtgacgc cgagcgccaa cgccctcctg tccttcgagg
    25321 ccccttcgta cgcagccacg atccccgctg gggggcaggg gacgtctttg gcccttccct
    25381 gcaccgtggg aaagggcacg gggttggcca cggtgacttt gacgacctct tggcccggag
    25441 gggagtcggt ccagacggtg gagcttcccg tgcgccccgc cgcccctcgc atcgtccttt
    25501 cgggggcggc ggccttcaag ggaggagaga agggggtctt ggacctgccc cggaagtggt
    25561 tcccggggac ggagcggggc cttctggtgc tgggatcctc tccccgggtg gagctggcgg
    25621 aagcggtgcg cttcctggag acctatccct acggatgcct ggagcagacc gtttcggggg
    25681 cctggcccct gttggtcctt cccgatctgg tggcggaagt ggaccccgag ctcaccgagg
    25741 cgggggagat ccgctcccgg ctggaggggg ccgtgcggcg gattctgacc ttccagaact
    25801 acgacggagg attcctcatg tggcccgagc aatcgggcgg agggtggtcc tggccgggaa
    25861 tctacgccct tcacttcctg gtggcggctc aacgcgaggg gatcgatgtt cccgagggac
    25921 cgctgaagga agccctggag ggcctccggc gccagatgac cgaacctttc tcgggggagg
    25981 ccctggcgga ggaagccctg cggaacaagg cccaccgagg ggcctacgcg gcctacgtct
    26041 tggccctggc gggggaacct cccttggcgt ggatggaagt gctccgagaa gatcgggccc
    26101 acctccaggg ggtggggaaa atcctcctgg cgggggccta tgccctggcg ggggagcgcc
    26161 agagcgcccg ggatctcctg gannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26281 nngggcgaag tcctcgccga ggccaccctg gatcaaggcg cccggcgggc ggtggaaaag
    26341 ctccctgcgg gggtgacttt gacctgcgag ggagaaggga aggtctacgc ctcctggacc
    26401 gtggagggag tgcccgaggg caaagtcact cccctatccc aagggatcgt gctggaacgc
    26461 gccttcaccg accgggaggg cgctcccttg ccggaaaacg cccccatacc ccagggggcg
    26521 ctcctgaacg tggtgctcac ggtgacctcc gaacggaatc tgtcgcagtg catcctggcg
    26581 gacctcctcc ccggagggat ggagatcgac aacccccgcc tcggggtctc ccagggagac
    26641 cgggaaaact ccgccatcgc ccacgtggag ccccgggacg accggatgct cgtcttcatc
    26701 gacgagctcc gaggagggga gcccctgacc tacacctacc gggtgcgggc cgtgagcaag
    26761 ggcacctttg tcctgccccc cgcctcggcg gagggcatgt acgaccccgc cgtccgggcc
    26821 ctggtgcccg gcggggtgtg gcgcatcgag taaggcctta ataaaaagga ccaaaggccc
    26881 gaggaggccc ctccggggcg ccccctcggg cctcgatgac atcggcttta aggccaaggg
    26941 gaaggcgtgg atggagagaa gatatgggcg gtggagacgg agggcgctgg cggcgaccct
    27001 gggtgtggcg gcggtgggat tggggttggg ggccttctgg ccccccaacc cggagggctc
    27061 tgtgggacgg acccccgcgt cctttcaggt gacggaccga tcgggggagc tcctccgggc
    27121 ctatctggcc cccgatgacg tctggtgcct tcccctttcc ctagatgcca tgggacgatg
    27181 gatccccctc atcgcggtgg aggtggagga ccggcgcttt ttttcccact ccggggtgga
    27241 tccgtgggcc ctggggaggg cggcgtggca gaatcttacc cagggaaagg tggtctccgg
    27301 ggcttccacc atcaccagcc agctggtgcg cctctcttcc cccacccccc gtccccggac
    27361 ctggccggtc aaggtggaag aattcctggg ggccctgacg ctggagcgcc atctctccaa
    27421 agaaaccatc ctggagagct acctgaacaa ggcccccttc ggggggaatc ttcggggggt
    27481 ggaggcggcc tcccgggcct atttcggcaa gcgggcccag tttttggccc cctccgaggc
    27541 ggccctcctg gtgggattgc tccagagtcc caccctcttt cgtcccgacc gccaccccga
    27601 ggcggcccgg aggcgccggg atcacatcct tcgcctcctg cggagccggg atctgttgga
    27661 tgatcaggcc ttggccctgg ccctgcggga tcccatcccc cccaagctct tccccttccc
    27721 cctgcgggcc ccccatgggg ccgacctcct gcgggccacc cggccggatg aaggggtggt
    27781 gcgctccacg ctggaggccc ccctccagcg caccgccgag cgccttcttc tcgaggccct
    27841 gacgcccttt ccgggctccg tggcggcggc ggccatggtg gtggaaaacg ccaccggggc
    27901 ggtgcgggcc tacgtgggca atgcccgctg ggggggggcg gatcggggaa attgggtgga
    27961 ttgcggcgac gtcccccgat ccccgggatc ggcgctgaag ccctttgcct acctgcgggc
    28021 cttcgagcgg ggtctcttgg cgccgggatc gatgctggcg gatactcccc tgggcatggc
    28081 gggaaggagc ccccggaact tcgaccgaaa gtaccggggg gcggtgagcg cccgggatgc
    28141 cctggcggat tccctcaacg tgccggcggt gagggtgctg cgcctgatcc gggggaacga
    28201 cctcctggcc cttctccggc ggtggggctt caacctcctg aggaaggact cggaccacta
    28261 tggcgacgcc ctggtgctgg gggggtgcga ggtgaccctc cgggagctgg cccaggccta
    28321 tgccgccctg ggagcggggg gagtttttca tcccctacga tgggaggagg acgcccccga
    28381 gatgccgggg gtgcgcctct gttccgccgc ctccgccttc ctcgtggcgg atattctgag
    28441 cgacctggag cgcctccccg ggctggtgcg ggacaagctc cgggggacgg ggctttcggt
    28501 ggcgtttaaa accggcacct cctatggcct tcgggacgcc tggaccgtgg ccttttcccc
    28561 tcggttcacc gtggcggtat ggtttgggga cacggggggt accccccggg atggcctggg
    28621 gggactcctt ttggccgcgc cccaggcggt gcgcctgctg gaggaggcgg agcggggcaa
    28681 gccccggaga tcctttgtcc cccctcccga ggtggggcgg cggcgggtgt gcaccctttc
    28741 ggggagcccc ctgggggagg cctgtcccgc cagccgcctg gagtgggaga tcgtgggggt
    28801 gtcccccggg gggagttgcc ccctgcacga gcgccgggga ggggaagtgg tgaccctttg
    28861 gcccccggag ctcgagggct ttctccgggg aggagtggcg gcgtggcagc gggccccctt
    28921 ggccgtggcc tcccccctgg cccacgggag atacgtcttc ctccccaccc cggagggggc
    28981 ccccaagatc cccctggtgg ccgagggggg agatcgggga gagctctttt ggttcgtgga
    29041 cggggtcttt ttcgcccgcc aggtccccgg ggagagcctc ttctggcccc tgcccgccgt
    29101 gcccggagaa catcgcgtct ctgtggcgga tgggtcgggg ggggccgcag gggtgaccat
    29161 cgtcgtggag acggggaaga ttccctaaaa agatggtccc ctccggcgcc gaggccctac
    29221 ggggcggagt aggtgtaagt tatcccggag aagggggcgg gggaggtgcg ggtgcgcacc
    29281 cggagggcgt cgtctcccat ctccaggaag gcgatcttca cgggaaagat gaaatccctg
    29341 aaatcgacaa cgggctggag cccctccagg gcctctcgga tgagcccctc gctctgggcg
    29401 tcgccgttga cggagaagga gtagtccgcg aagcagatct ggtttccccc ccggacctgg
    29461 agcttggtgc gaagctccac cagggcgctc accatgcccg aggcgtaccg agcccgggcg
    29521 caaagccccc ccggccggag atccacggtg gcgtccctcc actcgtcgct ccccttgccg
    29581 aaggcgtttt tccggagaaa gtcgttgagg tccgcctcgg tgatctccgc gtcgaacttc
    29641 gccgatacga tctctttcac ctggagcccc ccgggggagg cccagtcatc gatgggggag
    29701 aaggtgacga acgaaggctc cagccggagg cgggccaccc gaaaaccccc ggcggaggcc
    29761 cccaccacct cgaggaagag atgacgcacg gtgtgggtcc catcgggggg ggcgtccatc
    29821 accagcgtgg cctcctccgg gacgaagtgc tccaccagga cccggagcat ctcgctcccc
    29881 gggtcccctc cggaaggcac ctttcccacg aagtgagccc cctccgtcgc ccctccgggg
    29941 gatcccccca ccaggaggat caaaaggatc aggatggcgc
//
LOCUS       DAOOER010000024        28129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_1934_length_28129_cov_8.258745, whole genome shotgun sequence.
ACCESSION   DAOOER010000024 DAOOER010000000
VERSION     DAOOER010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 28129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28129
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1934_length_28129_cov_8.258745"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..342
                     /locus_tag="PL162_04865"
     CDS             139..342
                     /locus_tag="PL162_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HQF91386.1"
                     /translation="MATQGTVKWFNETKGYGFITTDDGKDVFVHYSAIQGDGFKTLGE
                     GQRVSFDITQGTKGDQASNVSKI"
     gene            577..837
                     /locus_tag="PL162_04870"
     CDS             577..837
                     /locus_tag="PL162_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HQF91387.1"
                     /translation="MEVLKVSAGSQPKSVAGAIAAVLRETGALEIQAVGAGAVNQAVK
                     SIAIARGYVAPNGIDLICIPTFSRIDIGGEERTAIRFQVEKR"
     gene            966..2231
                     /gene="hisS"
                     /locus_tag="PL162_04875"
     CDS             966..2231
                     /gene="hisS"
                     /locus_tag="PL162_04875"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HQF91388.1"
                     /translation="MESIKAPRGVRDILPEESWKWAHVMGVARKTAEDFGFEEIHLPL
                     FEHTELFCRGIGDTTDVVEKEMYTFTDRGDRSLTLRPEATASMVRAYLEHNFQHGPQP
                     AKFWCAGPMFRYERPQKGRYRQFWQLDIEAIGAADALVDVETIALSLEFYRRLGLSGL
                     EVVVNSVGCPRCRPVYRQALQAYLGERLTQLCDTCQGRFGRNPLRILDCKNSGCKALT
                     EGAPDIRDSLCPECRDHFALVLSALEAQHARVTLDKRLVRGLDYYTKTAYEVLSGDLG
                     AQNAVCGGGRYDNLAEAIGGPAVPGVGFAAGLDRVVLTMEQQGCSFGKRACPQAFVLC
                     EADTRAQGSALLAALRSAGVAADMDYTGKGFKAQMKLADTRGVRVACIFGGNEAARGV
                     VALKDLRTGTQESVPQEELAARVGAILKG"
     gene            2405..4219
                     /gene="aspS"
                     /locus_tag="PL162_04880"
     CDS             2405..4219
                     /gene="aspS"
                     /locus_tag="PL162_04880"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HQF91389.1"
                     /translation="MEGRHFDATWKRSVYCGEVGAPHEGQEVVLNGWLRRRRDLGGII
                     FFELWDHTGVVQVVVNPDEGGDLHRRAGDFRHEYVLAVRGLVRPRPEGTVNEAMATGQ
                     WEVAATDLLLLSPSRPLPFEVNDGDKVDENLRLRHRYLDLRRERMQSHLRIRSRAALF
                     TRNYFSSQGFVEVETPMLTRSTPEGARDYLVPSRVNPGHFYALPQSPQLFKQILMVSG
                     CDRYMQIVKCFRDEDLRADRQPEFTQVDLEMSFITEEDIYALLEGYIRGIYREVRSEE
                     IPTPFPRMPWTEAMDRYGIDKPDLRIPFEICPLEGVFSASSMEAFRGVLARGGAVRGL
                     PVPGGAVMSRKELAEVEARAKALGAAGLGTFTWKEGTLKGPLVKFLGADGERALVEAS
                     GLKDGDVLFVVADDDRLKACTVLGQLRLELARSRGLVQEGWRFLWVTEFPLFEWDQDE
                     GRWVAVHHPFTAPVTEDLPLFDSAPGRIRSRAYDLVLNGCEVGGGSIRIHDPAVQEKV
                     FGALAFSPEDARERFGFLLDALGSGTPPHGGLAVGFDRLVMLLCGGKSIRDVMAFPKT
                     QKAQCLLSGAPGTVDQAQLDELAIALAPSHETSGGSKE"
     gene            complement(4471..5337)
                     /locus_tag="PL162_04885"
     CDS             complement(4471..5337)
                     /locus_tag="PL162_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HQF91390.1"
                     /translation="MDAKQLQELMQGKIDDMPHKAHRVVAYLLSNMREASFRSIGEVA
                     DELRVSKAQLVRVARMLGFTGYAELKDALQSAILDQVNPAAMLVKSRDGEENLPHTIL
                     RMEHANLEDTWAQLDGDKSAQFCTMVQGAQTVYCVGWGISSLVAEHLFMRLRVMGVNG
                     FLLKRGSLTLTEQVRAVRPEDLMVVCELPSYVVEVTESIRDCADRGVPVISLTDSPAA
                     PTCRFASLHLFASAGSPTFGSSIVGPLFLVNVLTSLLALSLGDAAHQAMEAQATFLHD
                     TRIFHPIFGLKY"
     gene            complement(5707..6567)
                     /locus_tag="PL162_04890"
     CDS             complement(5707..6567)
                     /locus_tag="PL162_04890"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: protein motif:HMM:NF013991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
                     /protein_id="HQF91391.1"
                     /translation="MGMESKVLGALAAHAALWEIHVAPKPGLVDRLNRGAHGDMDYAL
                     FLRSAQALAPLWSLPGEVGLKGTPPEEALAPLRNHGIAMEGAMFQATGGVNTHKGLIY
                     VLSLLLYGAGRTVFLGLPPVPEVVCALGGEPCRNAVRDELVPLLAGDPHRPLTHGEAL
                     FRSHGVTGVRGEAARGFPSLRRGGVPAFFYSLRQGNSWHDAALDALLVLMEICEDSNI
                     MHRGGYDFWRGPYRQLVRASRRRLREGGPRGEILAALDETITSKRLSPGGAADLLTGT
                     LFLALVAPTP"
     gene            6887..7771
                     /locus_tag="PL162_04895"
     CDS             6887..7771
                     /locus_tag="PL162_04895"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQF91392.1"
                     /translation="MDVRELESLVAVVDKGTISRAGAFLGLSQPAVSKHIAKLEEELG
                     VSLLVRGHGRSVLTPEGKIVYGHAQAMLEIQRRVRRDIAEAGSAVTGLVRIAASSIPG
                     NYLLPPRLARFREEYPGTEVEVLVSHTRQALGDLVERRVDVAVVGSERFLPGVDMVPF
                     FTDEVVLVVSPQDPLARRPALSLEEAARALRVGRTEGSGTQIFVEEAFKARGVALPRP
                     PLRFDHASAVVGAVAAGGGPGIVSRQALPQGEDVVAIPLDPRIDRVFFLAHGALEFRA
                     VALLVDFLLRETKGENGR"
     gene            7768..9906
                     /locus_tag="PL162_04900"
     CDS             7768..9906
                     /locus_tag="PL162_04900"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HQF91393.1"
                     /translation="MRIFPDLLKRLGGAVLLAVAWASGAFGGAPSMEELFWKRAWGAV
                     EQRVIQEGNFLSLSDATFAANAFWMQKRYGDAVDLLAPRRHALPPEVRVYADLMLVLG
                     YERTNRREEALKLGKDLWPRTSADVRYYVAYALARIYRDEGKTSEAREWFRRTVASAE
                     DAASRLPALKALMELPGATVTDALRVLEDQPLNAQALEILKKEKKPSAGVLMALGYAA
                     HAKGKHKEAVELLEKARRLGAGERASFWYALSLYRLGRTAEAVKVWTPVALSGDRYAH
                     RAAERLGIAAGKGQAQVAAAVLEKVVQERRGRIRQVALFQLIKIAREYGVGDEAAWKQ
                     RLLRDEPGSSAAVEILWKDGLAAWDRGDLVQARDFWSRGAAFGTGAQWPPRFLYWLAR
                     VDDRAGRTASADASRRKLGYQYPTSTYAFRVFPRGSKPLSDDVPPSLRGTPSLLEKWG
                     FIQYARLTLARSPSAADRFRAARIARWMEDDRGAYSLAGTLTGALATEPWMSRELLRM
                     LYPRPYEKTVLAAGVSFDVSPELLWAVMRQESGYNPSVTSHVGAMGLMQLMPATAKDE
                     AQRLKIPLKDAYQVDTNITLGAAHLARHLRRFVEPAKAAAAYNAGGGSVSRWEGRGRD
                     LEEWIEAIPFLETNDYVRRVMGNWETYRLIYGDSPRRKASPDQGSRGGESPLASEDFP
                     PEAGLSPDLSPEDAPEVPGEPPSGGENQGG"
     gene            9914..10405
                     /locus_tag="PL162_04905"
     CDS             9914..10405
                     /locus_tag="PL162_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HQF91394.1"
                     /translation="MRQNDPSDPVVRVRAFLERAGYRGSIHETEDTIFTVEDASRAVG
                     APPEEILKSLVLMVDGAPVLALLSGVNRVDLRKVQRLRGARKVKMADAEWVFSYSGFR
                     IGGVPPVGYPEQLPALLDEDLYRHAVVWAAAGTDHAFFPVPPGELERLTGGQRADIRK
                     ENV"
     gene            10526..11104
                     /locus_tag="PL162_04910"
     CDS             10526..11104
                     /locus_tag="PL162_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HQF91395.1"
                     /translation="MDWGLALALGLSLSLDAFAVSCAIPFAVPQISWPQALRVAGTFG
                     IFQALMPLAGWMAADLAADLIAPVDHWVAFGLLSLVGGRMIGEGWAKMRSPGRCQTLS
                     GDPTRGRRLLALAVGTSIDALAVGVSFIGLRIAVYRTVTVIGMVTFLTCMAGLLASKR
                     LCGEGGGRWEIVGGGVLVAMGIKILGDHLGFF"
     gene            11152..12297
                     /locus_tag="PL162_04915"
     CDS             11152..12297
                     /locus_tag="PL162_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91396.1"
                     /translation="MRRLGRATTMWGTILGFLVAVGVAGGPAEAQGIPIHRDRGMEVF
                     LVACDGSTDPASATVLVDLKSDELRREAAAQLEALDRAPEEASTVARRVTTFLFGAEG
                     KRWRIQRETFLGDGGKKLAETEDRGEGPWADMKPGSLPDQIWRAVETMKKNGVKWAVP
                     PALYLPSGEIPGRSPSQDDAPSEVAYRDDRVYPLFREGGSYVWVELDWSECLMRQSDS
                     PAPRALEAMERVRSFALARHLEDGRWRVTLQGETRDRTIFLPDEGAFLALEGFLREGL
                     ENRRVRRAHGDSSQAPLAAGEGGEGPAEMVLCGVSGDMNEEELLEVRWLGAPGKGTVV
                     GTAEAWRYLADHLFLARRRWEHQQRGIYDFVGKLERLGIGKAPSPRP"
     gene            12460..12633
                     /locus_tag="PL162_04920"
     CDS             12460..12633
                     /locus_tag="PL162_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQF91397.1"
                     /translation="MAKAVVDRDTCVGCEACVGACPVSAISMQDGKALVDAGSCVECG
                     ACVSTCPVSAISQ"
     gene            12752..13057
                     /locus_tag="PL162_04925"
     CDS             12752..13057
                     /locus_tag="PL162_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91398.1"
                     /translation="MVWMRQKAWWNKATGPGGKAETAESGDNSGLPLSRPLDYQKLEN
                     ALRHLRWARMMLHEMSGAHNGEKLGWELYDMAQRVNGDEREVDALLKKFRSEGIPGA"
     gene            complement(13164..14225)
                     /locus_tag="PL162_04930"
     CDS             complement(13164..14225)
                     /locus_tag="PL162_04930"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HQF91399.1"
                     /translation="MSESFDKVRQLLGSEAEYLLSHTCGTIPSSSLHLPGPDFVDRVM
                     AVTDRSVPVLRSLQTLFNAGRLGGTGYISILPVDQGIEHSAGASFAPNPMYFDPENIV
                     RLALEAGCNGVASTLGVLGAVARKYAHKIPFIVKLNHNELLTYPNTYDETMFASVEQA
                     WDMGAVAVGATIYYGSSESRRQIMEISEAFERAHDLGMATILWCYLRNSAFSTPEKDY
                     HVAADLTGQGNHLGVTIKADIIKQKLPENNGGYPALKFGKTHKAVYEKLSSDHPIDML
                     RYQVANCYMGRAGLISSGGAAGANDLADAVKTAVINKRGGGMGLILGRKAFQKPFAEG
                     VALIQAVQDVYRDQEVSIA"
     gene            14356..15081
                     /locus_tag="PL162_04935"
     CDS             14356..15081
                     /locus_tag="PL162_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91400.1"
                     /translation="MKEPKKMAVFLRACVLGVLLGWAFPALADGVFGSRLQEDTTRRF
                     ENYLKGEIRAERLQVALRGTDGDLQAGRVPEVRIFCSGALLGDLRCDRLMVVLRSVTF
                     RPSGQGVLLENPGAAEVSGTILEKDCEQSLKKSFPDLKNAEVRLEGSRVVVSGAYAKN
                     YVVPVRAFVKLYGKYVIDPQSGEASLAFEKATNDNPFISTEDIAKSLSRKSLRLSLAE
                     FFVKPSLRDVRVDKRMVWFSTKP"
     gene            15212..16096
                     /locus_tag="PL162_04940"
     CDS             15212..16096
                     /locus_tag="PL162_04940"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQF91401.1"
                     /translation="MDPASTVVGFVGTGVMGASMARHVMRAGYAVRVYNRSRSKAEAL
                     LAEGATWDDTAGSLAASSAVVITMVGYPADVEAVYFAPEGILASAKPGTYLVDMTTSS
                     PELASRIARAARERGLHALDAPVSGGDRGAREGTLSIMVGGEAEDLETLRPLLGTMGK
                     QVVLQGPAGSGQHTKMANQIAIAAGMIGVCEALAYAQRASLDPDTVLQSIAGGAAGSW
                     TLSNLAPRMLAGDFRPGFYVKHFIKDMNIALESAAALGLSTPGLALARELYGQLAEAG
                     GEEQGTQALFRLFSDRRA"
     gene            16216..17385
                     /locus_tag="PL162_04945"
     CDS             16216..17385
                     /locus_tag="PL162_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HQF91402.1"
                     /translation="MPVRPIYLDHSATTPLDGVVLEAMMPYLRENFGNPNSIHAWGRR
                     ARQAVEEARGAVASLVGAKDREVFFTGGGSEADNLAIKGTALARQQKGRHLITSAIEH
                     HAVLDVFSWLGKQGFDVTVLSVDETGRVSPKDLERALRPDTTLVSIMFANNEIGTLQP
                     VAELGELCRQRDVLFHTDAVQVVGHLPLDLATLPVDLLTMSAHKMYGPKGVGALFVRR
                     GVRIDPLVHGGGQERGLRSGTENVAGIVGFGAAARLARERLAAGEHLKEAELRDHLLE
                     GLLEVDDVLLTGHRTDRLPFHASVCVRFIEGEALLLRLDAAGIGASSGSACTSGSLDP
                     SHVLLALGLDHATAHGSLRLSLGKDTSREDVDRTRAAFREIVEVLRAMSPFGRNK"
     gene            17415..17786
                     /gene="nifU"
                     /locus_tag="PL162_04950"
     CDS             17415..17786
                     /gene="nifU"
                     /locus_tag="PL162_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HQF91403.1"
                     /translation="MYSEKVVEFFMHPRNVGRLDPADGVGEVGNPRCGDVMKIYIQVR
                     DNRIDDIRFETFGCAAAIATSSMITEMVKGKTLEEALEVSNEDVLDALGGLPPEKIHC
                     SLLAEEGIRAAVEDYRKRQSP"
     gene            18033..19988
                     /locus_tag="PL162_04955"
     CDS             18033..19988
                     /locus_tag="PL162_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91404.1"
                     /translation="MWKEHPWGTLALLALAGAMAFLARFEPPLLRQGDRLWQDYHMAT
                     RPLSEPSQPIIIMSLESSPGGSFDAPAVLPEILGRLREARAVVISPGVELRLDASGDQ
                     KIAEAARRAGNVVGGVALGGGGGTGGWVRFAAVTGVELQEADSDGRYRSFSPFALVEG
                     KMVPSLALAAYLHVTRDVPRVKESPSGLRVLALGDRRLPLDFQGRLPVAIPPEGAGPS
                     SFVVRSKDLSLPEDTLRRAIVVLELPSRQRRTMPLPAFSPEAHASAGNFLAWSIGTLF
                     VPALPQSAPWIWPAALAWGGMALAVALVWWMPLGAVWAGVLGVLVLVVGCAAVVFSAT
                     LICLSPLVPLLGVLTAGSVAVGLRWIRHRRERALAILAGDAVRSFLEGLPADDATFEE
                     CLRKLWPDLERLTGVRLLGAFLPQGIIEEETLDGGTVLARSPHLYLVRNDVDPPRYRL
                     YARQDFSLAEGEPRFVALGWDRLMPPETLRSLALMLSLSSWFYAGTRASERRLFHFRQ
                     ALGGILRKKTRNAPMPLVAVVDELARQLRWHDSRREDLHTLAVLEEELTPHPDDPPEV
                     EAPSAVGFLKSSEGNPLEAVLEAARAVVREMPSAGSSPGALRDRMRGALRGPGMAAFS
                     PELLTALEEMDYGTVLTPRDPLPPPGP"
     gene            complement(19951..20742)
                     /locus_tag="PL162_04960"
     CDS             complement(19951..20742)
                     /locus_tag="PL162_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQF91405.1"
                     /translation="MPDTPQTFPEPSARFRHLVGKALADFNMITPGSGILVGLSGGKD
                     SLVLLEALGALRSRSPVPFTLRACTLDPSMGALDSSFIEARCEALGVPLSLISHDILG
                     IIRQRQERSPCSFCATMRRGILCSAAASAGCTTLALGHHLDDVVETTLLNLVSGGRFQ
                     CFAPRVWHSRSGITVIRPLVYVAEGHIRAEIQRLGLRPVAPPCPYAGGTERARIKELL
                     ALLQDRYPHIRSNVLHALKNLRDGGGWEVSPRKAMAPGEEGDLEG"
     gene            20926..21591
                     /locus_tag="PL162_04965"
     CDS             20926..21591
                     /locus_tag="PL162_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQF91406.1"
                     /translation="MKKIRLVVADGHRLFREGLCKLLSMEPDMEVVGEAEDGDEAFRT
                     VRDLGPDLFLFDVGLPGGNSVSLVRDLRSQRGLRFVAITSYGDQTHLTALALAGVHGC
                     LVKTSGLEELLAAVRMVSQGIPYVDQRTAGRMIFPDPRSPSGKDAFLGLTAKEKETLF
                     WLSQGFSNADIAERMVVSEKTVKNHVSHMLKKLDLRDRTQAAVLAWRMGLAQLAPEVL
                     GVR"
     gene            complement(21711..24212)
                     /gene="fliD"
                     /locus_tag="PL162_04970"
     CDS             complement(21711..24212)
                     /gene="fliD"
                     /locus_tag="PL162_04970"
                     /inference="COORDINATES: protein motif:HMM:NF018852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HQF91407.1"
                     /translation="MVNSMFQVTGAGSGIDWGGMIDKIMENARKIEKPWKEEKEKLEL
                     KIGLYEELSGGLKQLQKILTSLKLPSEYNKKTAVFSVLSPAGAKAESILTATPTSEAV
                     IQEWDLDVLNTATAERRISNYYSSATTALGLSGSFAIRVGGQRATIAVATSDTLRDIN
                     YKIKQAVDSGNNRLNISAQLVNNRIVLSSANSGLGSASATGATMRRGTWSAATGGDDY
                     LPHKMDVAYTRGTGSTDQITPLTSGQYPSTISIEGKTATTDYTYDNSTGTISWVAGHE
                     PATGTDFKVTYEGGYPDYIAEIKSGSTTYTSGTDFTYDGATGKIHWNAGANHPAEGAS
                     YEITFKSTLNKTKGSAEGTDTLTRPSSGVFPPTIVVKSGSTTYTADTDYHWDPVTGTI
                     DWSLGGGEPPTGSSYTVTFGREYTYAANPFYLEDETGGVLSSLGLAGNLGSSYSKATD
                     ANLLLNGVPVTRASNTITDLVGNTTLNLKGAGHVTMSVEADAEKAVADVQSFVELYND
                     VMEWINVRISEESKKTSKSNSNKSDDFNKKFGLLHGDSLLWQTKARMRQLISDPLNIA
                     GPLRSLSQVGIATEKANYGKSGKLEFDTQLFMDALTPGKMSFMDEWMTDQKTSATESL
                     GKIVAGFTGGNFSITQDGRTATVNITTDDSLTSMAQKINDARTEDGQSIGVSASVIDR
                     KLVIRSTKMGKELSFTDGNAVLKKLGIDVTNPQDRTHRVLDTPPYVGELMTKAMTQLD
                     AYMDNLVKSTQVQVGPTTAPQGRVASQILYLKNQVAAIDLRVKNYEIRMDLMEQGLWT
                     RFTTMEKALTKVNAQASALASAFASLSGASKASSQ"
     gene            complement(24485..25279)
                     /locus_tag="PL162_04975"
     CDS             complement(24485..25279)
                     /locus_tag="PL162_04975"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQF91408.1"
                     /translation="MKDVQATILQRASLRQYDPTPLSEEDREQILLAAERAPTAGNMM
                     LYTILEVRDQRIKDVLAESCDHQPFIARAPLVLVFLADFQRWQDFYVASGVEDLCTRR
                     EESFRRPQESDLLLACCDALIAAQNTVIMAESRGIGSCYIGDIMERYEQHRELFSLPP
                     WAFPITMLCYGRPPAGYRQNLRPRLDRKFFVQQDRYRPWSQEDLEDMAHQPALLPKKL
                     REGASNPGQHWYLRKTGEAFSREMERSVRVALRRWLDGDPEDGGRS"
     gene            25609..26463
                     /gene="panB"
                     /locus_tag="PL162_04980"
     CDS             25609..26463
                     /gene="panB"
                     /locus_tag="PL162_04980"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HQF91409.1"
                     /translation="MGKKKTRLDFVDMKKRGEKVAWITAYDFPTASFAEAAGMDMILV
                     GDSLGMVVLGYDGTVPVTMEDCIRHSQAVRRGAPQTFCIGDMPFLSYQISDEDAVRNG
                     GRFLKEASMDAVKLEGGRRVARRIRALTDAGILVMGHIGLTPQSSGQLGGFKAQGRDP
                     ESARELIADALAVQEAGAWSLLLEAVPPELTAFLAKKLTIPVYSIGAGAPCDGQLLIC
                     GDMLGLFQAFTPKFVKKYAQLAELETEAFRAYVEDVKSGAFPGDEHVYHILPGREKDF
                     EELFREFP"
     gene            27248..>28129
                     /locus_tag="PL162_04985"
     CDS             27248..>28129
                     /locus_tag="PL162_04985"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQF91410.1"
                     /translation="MRKWMILVGLVALGVLGVAWNSGRLFPGLGKSLERSRAEEGVTA
                     SGTVEIRTVEVASKLGGYLRELRVREGDLLAAGELIGGVDRPDLEAQISRDEASLRRA
                     RAHLADLQSGSRPEEIAEAAARVASARSRYEKSRQDFNRFESLHRDKVISDRQFDEYR
                     AAFDGAKAELAAAQDRHALLRAGARPLQVEAQRAEVEALEAALALTRSLWADCQIRAP
                     QGGVVLSKGFERGEYVAPGGVLVTLGDPMDCWVRMYIPSAQVGLLRRGQRAEVRVDAY
                     PHRTFSGQVTEIGQEAEF"
BASE COUNT         4743 a         8550 c         9744 g         5092 t
ORIGIN      
        1 aacccggtag atctttcaga aagtcccctc atgcctcggt cgggaatttt tcgcttgaca
       61 gaaaaggcct ggccccctat catagggtcg ccggtagtcc tatccggcaa acgtttttca
      121 gcacgaggag gctgtgtttt ggcgactcag ggtactgtca agtggttcaa cgaaacgaag
      181 gggtatgggt tcatcaccac ggacgacggc aaggatgtgt tcgtccacta tagcgccatc
      241 cagggcgatg ggttcaaaac gctgggcgaa ggtcagcgcg tgtccttcga catcacccag
      301 ggaactaagg gagaccaggc gtccaacgtt tccaagatct aggggcggac tttttcttcg
      361 atcatattat tatacggtag tgtgatttgg agaaagacga gagatgaagg cgggggctcc
      421 gatggggcct ccgcctttcg cgtaaccccc tttagggaag ggggcgggga gccctccggg
      481 acgccccggg ggcgctcttg tcaacggtcg ggggaagagg atataattta tcctggctgt
      541 ggcggcccag cacgagaagc gtaagggggg atatccgtgg aagtgctcaa agtgagtgcg
      601 ggatctcaac ccaagtccgt ggcgggagcc attgcggcgg tactccgcga gacgggggcg
      661 ctggagatac aggcggtggg ggctggggcg gtgaaccagg cggtgaagtc catcgccatc
      721 gcccgggggt acgtggcacc gaacggcatc gacctgatct gcatcccgac cttttcccgc
      781 atcgatattg gcggcgagga gcgtaccgcc atcaggttcc aagtggaaaa gcgctgattt
      841 cgcggggtac gggaagggga agaagctggg ggcggcccgt cgggctgccc ccagttatca
      901 taggggggag acgggcccca aggctaggat cgaccggcat cgtccggcga agggaggaaa
      961 ggcgtatgga gagcatcaag gcgccccggg gagtgcgaga catccttccg gaggaatcct
     1021 ggaagtgggc ccacgtgatg ggggtggcga ggaaaacggc ggaggacttt ggcttcgaag
     1081 agatccatct gccccttttc gagcacacgg agctcttttg ccggggcatc ggtgacacca
     1141 ccgacgtggt ggaaaaggaa atgtacacct tcacggaccg gggggatcgc agcctgacct
     1201 tgcgccccga ggccacggcg tccatggtgc gggcctatct ggagcacaat ttccagcacg
     1261 gcccccagcc cgccaagttt tggtgcgcgg ggcccatgtt ccgctacgaa cggccgcaga
     1321 aggggcgcta tcgccagttc tggcagctgg acatagaagc catcggagcg gcggacgcct
     1381 tggtggacgt ggagaccatc gccctttccc tggagtttta ccgtcgcctg gggctctcgg
     1441 ggctcgaggt ggtggtgaac tccgtggggt gcccccgctg tcgcccggtc taccgccagg
     1501 cgcttcaagc ctacctcggg gagcgcctga cccagctctg cgacacctgc caagggcgct
     1561 ttggccgaaa ccccttgcgc atcctcgact gcaaaaattc cgggtgcaag gctctcacgg
     1621 aaggggcccc ggacatccgg gacagcctct gccccgagtg tcgggatcac ttcgccctgg
     1681 tgctttccgc cctggaggcc cagcatgccc gggtgacgct ggacaagcgc ctggtacgag
     1741 ggttggatta ctacaccaag accgcctacg aagtgctctc gggggacctg ggggctcaga
     1801 acgccgtgtg cggcggggga cgttacgaca acctggcgga ggccatagga ggccccgccg
     1861 tgcccggggt gggctttgct gcgggcttgg accgggtggt gctcaccatg gaacagcagg
     1921 gatgttcctt cgggaaaagg gcttgccccc aggccttcgt gctctgcgag gccgacaccc
     1981 gagcccaggg atcggccctc ttggcggccc tccggagcgc gggagtggcc gcggacatgg
     2041 actacaccgg caagggattc aaggcccaga tgaagctggc ggacacccgg ggagtgcggg
     2101 tggcctgcat cttcggagga aacgaggcgg cccggggcgt ggtggccctg aaggacctga
     2161 ggacggggac ccaagaaagc gttccccagg aagagctggc ggcccgggtg ggggcgatcc
     2221 tgaaagggta acccggagaa agggctcgcc ccgggacctg cggggatctc ggggcggctt
     2281 ttccagaggg gaagggcccg cgccgtgggg cgagaaggcc cccgtcgccc cgctcgggcc
     2341 ccgggtgcca cggggtttgt tttggatttt taggttcaaa cgaaaggggc aaggcaaata
     2401 gatcatggaa ggacgacatt ttgacgccac atggaagcgc tcggtgtact gtggagaggt
     2461 gggggctccc cacgaaggac aggaggtggt gctcaacgga tggctccggc ggcgccgcga
     2521 cctggggggc atcatcttct tcgagctctg ggaccacacg ggggtcgtgc aggtggtggt
     2581 caaccccgac gaagggggcg atctccaccg gagggccggg gactttcgcc atgagtatgt
     2641 gctggcggtg aggggcctgg tgcgcccccg ccccgaaggg accgtcaacg aggccatggc
     2701 cacgggccag tgggaggtgg cggcgacgga cctattgctc ctttccccca gccgcccctt
     2761 gcccttcgag gtcaacgacg gcgacaaggt ggacgaaaac ctgcgcctgc gccatcgtta
     2821 cctggacctg cggcgggagc gcatgcagtc ccacctccga attcgttccc gggcggccct
     2881 gtttacccga aattactttt cgtcccaggg ctttgtggag gtggagaccc ccatgctcac
     2941 ccgctccacc cccgagggag cccgggacta cctggtgccg agtcgggtca acccggggca
     3001 cttctatgcc cttccccagt cccctcagct ctttaagcag atactgatgg taagcggctg
     3061 cgaccgatac atgcagatcg tcaagtgctt tcgggacgaa gacctccggg cggatcgcca
     3121 acccgaattc acccaggtgg acctggagat gagcttcatc accgaagaag acatctacgc
     3181 cctgctggaa gggtacatcc ggggcatcta ccgggaggtg cgatccgaag aaattcccac
     3241 cccctttccc cgtatgccct ggaccgaggc catggatcgc tacggcatcg acaagcccga
     3301 tcttcggatc cccttcgaga tctgccccct ggagggcgtc ttttccgcct cctccatgga
     3361 ggcctttcgg ggcgtgctgg cccggggcgg agcggtgcgg ggcctccccg tgcctggggg
     3421 ggccgtcatg tcccgcaagg aattggccga agtggaggcc cgggccaagg ccctgggggc
     3481 cgccgggctc gggaccttca cgtggaaaga aggaaccctc aaaggccccc tggtcaagtt
     3541 cctcggggcc gacggagaaa gagccctggt ggaggcctcg ggcttgaagg atggggacgt
     3601 cctcttcgtg gtggccgacg acgataggct caaggcctgc accgtgctgg ggcagcttcg
     3661 gctggagttg gcccgatccc ggggcctcgt gcaggagggg tggcgtttcc tttgggtgac
     3721 ggagtttccc ttgttcgagt gggatcagga cgaggggcgc tgggtggcgg tgcaccaccc
     3781 ctttaccgcc cctgtgaccg aagacctccc ccttttcgac agcgccccgg ggcggatccg
     3841 ctcccgggcc tacgacctgg tactcaacgg ctgtgaggtg ggaggaggat ccatccgcat
     3901 ccacgacccc gccgtgcagg agaaggtctt tggcgccctg gccttttccc ctgaggacgc
     3961 ccgggagcgc tttggcttcc tcctggacgc cctggggagc ggcacccctc ctcacggggg
     4021 gctggcggtg ggctttgacc gcctggtcat gctcctgtgc ggggggaaat ccatccgaga
     4081 tgtcatggcc ttccccaaga cccagaaggc ccagtgcctc ctgtcggggg ctccggggac
     4141 cgtggatcag gcccagctcg acgagctcgc catcgccctg gccccatccc atgagacgtc
     4201 gggaggatcg aaagaataaa aataacgttt ctaggacctg cggccttcct cgtggaggcc
     4261 ccggggctct ccaccgtggt gatcctgccc cccggggaga gcctgaagcc ctcccagacc
     4321 actgaaggag cgggggcgag agaccgtggt gacgacaggg cggcccctcc gcccgccctg
     4381 ttcgaagggg gagggacgga ggggccgcct ccgggtcgcg gtctccaggg cctgagggag
     4441 gcttaggtgg cggtggttgg agggctcggc ctagtacttg aggccgaaga tggggtggaa
     4501 gatccgggta tcgtgcagga acgtcgcctg ggcctccatg gcctggtggg ccgcgtcccc
     4561 cagggatagg gccaggaggg acgtgaggac atttaccaaa aagaggggac ccacgatact
     4621 gctaccgaag gtggggctcc ccgcgctggc gaagaggtgg agggaggcaa aacggcaggt
     4681 gggggccgcc gggctgtcgg tgagggatat gacggggacc ccccggtcgg cgcagtctcg
     4741 gatgctttcc gtcacctcca ccacgtagct gggcagctcg cagaccacca tgagatcttc
     4801 ggggcggacg gcccgcacct gttctgtcag ggtcaaagac ccccgtttca ggaggaatcc
     4861 gttgaccccc atgacccgga ggcgcatgaa aaggtgctcc gccaccaggg aggaaatgcc
     4921 ccaccccacg cagtagaccg tctgggcccc ctggaccatg gtgcaaaact gggcagattt
     4981 atccccgtcg agctgggccc aggtgtcttc cagattggcg tgctccatgc gcaagatggt
     5041 gtgaggaagg ttttcttccc cgtctcggga cttgaccaac atggcggcgg ggttgacctg
     5101 gtccaagatg gcggactgga gggcgtcttt cagctccgca taccccgtaa aacccagcat
     5161 gcgggccacg cgcaccagtt gggccttgga gacgcgcaac tcgtccgcca cctcgccgat
     5221 ggagcgaaag gaggcctccc gcatgttgga aagcaggtag gccaccacgc ggtgggcttt
     5281 gtggggcatg tcgtcgatct ttccctgcat gagctcctgg agctgcttgg cgtccatcta
     5341 cccgtcacct tctcggaaaa aaataaacaa aaattacccg ttagtatact cggatggggg
     5401 gaaaagtcaa actccggggc atcctcgggg taaggggccc ttccccggga gcagggggat
     5461 gggaaaggga agttttgttt caagggccta gcccttcccg tctggggcgg cggggaggaa
     5521 ggttttccct agggctccgg gatgaagcga ggggaccgcg tcctcattcc gaagcccccg
     5581 catccgggga aaaggggctt tttctagccg gtccaaaggg gacggcccac atcgggtgat
     5641 gccccgcttc cgggtcgctt agggaggccc ggcgtagcga ccaagccctc ctgaccttgg
     5701 gccttctcat ggagtggggg ccaccagggc caggaatagg gtgccggtga ggaggtcggc
     5761 ggcccctccg gggctcaacc ttttggaggt gatagtttca tccagggcgg ccaggatctc
     5821 ccctcgggga cccccttcgc ggaggcgccg acggctggcc cggaccagct gcctataggg
     5881 gccccgccag aagtcgtatc cccctcgatg catgatgtta ctgtcctcgc agatttccat
     5941 gaggactaaa agggcgtcca aggcggcgtc gtgccaagaa tttccctggc ggaggctgta
     6001 aaagaaggcg gggacgcccc ctcggcggag cgatggaaag ccccgggcgg cctctccccg
     6061 gaccccggtg accccgtgag accgaaagag ggcctcgccg tgcgtcaagg ggcgatgggg
     6121 atccccggcg aggaggggga ccagctcatc tctcacggcg tttcggcagg gttctccccc
     6181 cagggcacag acgacctctg ggacaggagg gaggcccaga aagaccgtcc gaccagcgcc
     6241 gtagagcagc aaggaaagga cgtagatcaa ccccttgtgg gtgttgaccc ccccggtggc
     6301 ctggaacatg gccccctcca tggcgatgcc gtggttcctc aggggcgcca gggcttcctc
     6361 cggcggagtc cccttaagcc ccacctcccc cggaagagac caaaggggag ccagagcctg
     6421 ggcgctccgg aggaagaggg cataatccat gtctccgtgg gccccccggt tcaagcgatc
     6481 caccagtccc ggcttagggg cgacgtgtat ttcccacagg gcggcgtggg ccgcgagggc
     6541 ccccaatacc ttggattcca ttcccatggc gcgttttctt cccctctttc cgacgcggcg
     6601 tcccggggcg gcgtcttttt ccagggtagc ggatggggag gctccggcgc aaggggggag
     6661 aagaagctgt gctacaatga cccctggggg caggccctcc gaggctaaag atatcccgcg
     6721 agaaccccat tttcctccgg ggacagccgg ggtaaaatcg cctcgacggt cgaaggggag
     6781 cctagggggc gtggagtttt gcggggcccg ttcgacccct ccgccgtgcc ctctggggcg
     6841 aggggccaag ggacgggatc ccccggaggt gaaaggggcg aggccggtgg acgtgcgcga
     6901 actggagagc ctggtggcgg tggtggacaa gggaaccatc tcccgggcgg gggccttctt
     6961 ggggctctct cagcctgcgg tgagcaagca catcgccaag ctggaagagg agctgggggt
     7021 atcccttttg gtgcggggcc acggacggtc ggtgctgact cccgaaggga agatcgtcta
     7081 cggccacgcc caggccatgc tggagatcca gcgccgggtg cgccgggaca tcgccgaggc
     7141 tgggtcggcc gtaacgggcc tcgtgcggat cgccgccagc tccatcccgg gcaattacct
     7201 cctcccccct cggttggcga gatttcgaga agaatacccc gggacggagg tggaggtcct
     7261 ggtgtcccat acccgtcaag ccctgggaga cctggtggag cgccgagtgg acgtggcggt
     7321 ggttgggtcg gaacgcttcc ttccgggggt ggatatggtg ccctttttca ccgacgaggt
     7381 ggtcctggtg gtctctcccc aagatcccct ggcccggcgc ccggcccttt ccctggaaga
     7441 ggcggcgagg gcgctccggg tgggtcgtac cgaggggtcg gggacccaga tctttgtgga
     7501 agaggccttc aaggcccggg gggtcgccct tcccaggccc cccctgcgct tcgaccatgc
     7561 ctcggcggtg gtgggggcgg tggcggcggg gggggggccg gggatcgtct ctcggcaggc
     7621 cctgccccaa ggagaggacg tggtggccat ccccctcgat ccccggatag atcgggtctt
     7681 tttcttggcc cacggggccc tggagttccg tgcggtggcg ctgttggtgg actttttgct
     7741 aagggaaaca aaaggagaga atgggcgatg aggatttttc ccgacctctt gaagcggctg
     7801 ggcggggcgg tgcttctggc ggtggcgtgg gcttcggggg ccttcggcgg cgccccctcc
     7861 atggaagaac tcttctggaa aagggcttgg ggggccgtgg agcagcgggt gatccaggag
     7921 gggaacttcc tctccctcag cgatgccacc tttgcggcca atgccttctg gatgcagaaa
     7981 cgctacggcg acgccgtgga cctcttggcc ccccggcgcc acgccttgcc cccggaggtg
     8041 cgggtctacg cggacctgat gcttgtcctg gggtatgaga ggacgaaccg ccgagaggag
     8101 gccttgaaac tcggaaaaga cctctggccc cggacgtcgg cggacgtgcg gtactacgtg
     8161 gcctatgccc tggcccgaat ctaccgggac gaagggaaga cctccgaggc ccgggaatgg
     8221 ttccgtcgta cggtggccag cgcggaggat gccgcctccc gcctgcccgc cctgaaggcc
     8281 ctcatggagc tccccggggc cacggtgacg gatgccctgc gtgtcctgga ggaccagccc
     8341 ctgaacgccc aggccttgga aattctaaaa aaagaaaaga agccctcggc gggggtcctg
     8401 atggccctgg gctacgccgc ccatgccaag ggaaagcata aagaagccgt cgaactcctg
     8461 gagaaggccc ggcgtctcgg ggcgggggag cgggcctcct tctggtatgc cctttctctc
     8521 taccgcctgg ggcgcacggc ggaggcggtg aaggtgtgga cccccgtggc cctttcggga
     8581 gatcgctacg cccaccgggc ggcggaacgc ctgggcatcg ccgcaggcaa ggggcaggcc
     8641 caggtggcgg cggcggtgtt ggagaaggtg gtccaggagc gccgggggag gatccgccag
     8701 gtggcgctct ttcagctcat caagatcgcc cgggagtacg gcgtgggcga cgaggcggcg
     8761 tggaaacaac gcctgcttcg ggacgagccc ggctcatcgg cggcggtgga aattctgtgg
     8821 aaagacggtc tggcggcttg ggatcgagga gacctggtcc aggcccggga tttttggtcc
     8881 cggggggccg ccttcggtac gggggctcaa tggcctccga gatttcttta ttggttggcc
     8941 cgggtggacg accgggccgg gcgcaccgcc tcggcggatg cctcgcgccg caagctgggt
     9001 taccagtatc ccacctcgac atacgctttc cgggtctttc cccgggggag caaacccctg
     9061 tcggacgacg tgcccccctc gctgcggggg accccttccc tgttggaaaa atggggattc
     9121 atccagtacg cccggctgac cttggcccgg agcccatcgg cggcggaccg cttccgggcg
     9181 gcccgcatcg cccggtggat ggaagacgac cggggggcct actccctggc ggggaccctc
     9241 accggggccc tggcgacgga gccctggatg tcccgggagc tcttgcgcat gctctacccc
     9301 cgcccctacg aaaagaccgt cctggcagcg ggcgtgagct ttgacgtatc tcccgagctc
     9361 ctctgggcgg tgatgcgcca ggaaagcggc tataatccct cggtgaccag ccatgtgggg
     9421 gccatgggac tcatgcagct catgcccgcc acagccaaag acgaagccca gcggctgaag
     9481 attcccctaa aagacgccta ccaggtggat acgaacatca ccttaggggc ggcccatctg
     9541 gcgagacacc tccggcgttt cgtcgagccc gccaaggcgg cggcggcgta taacgcgggg
     9601 gggggctccg tctcccggtg ggaggggcga gggcgggatc tggaagagtg gatcgaggcg
     9661 atccccttcc tggagaccaa cgattacgtg aggcgcgtca tgggaaattg ggagacctat
     9721 cgtctgattt acggcgattc cccccgacgg aaggcctccc ccgaccaggg gagccgagga
     9781 ggggagagcc ccttggcctc ggaggacttc ccgccggagg cggggctttc cccagacctt
     9841 tccccggagg acgccccgga ggttcccggg gagcccccct ccgggggaga aaatcaggga
     9901 ggttgatggc ttgatgcggc aaaacgaccc ttccgacccg gtggtccggg tgcgggcctt
     9961 tctcgaacgg gcgggataca gggggtccat tcacgagacg gaagacacca tcttcaccgt
    10021 ggaagacgcc tcccgggcgg tgggggctcc gccggaagag atattgaaga gcctggtgct
    10081 gatggtggat ggcgctccgg tgctggcact cctgtcgggg gtgaaccggg tggaccttcg
    10141 gaaggtgcag cgcctccggg gggcccgcaa ggtgaagatg gccgatgcgg aatgggtctt
    10201 ctcctactcg ggctttcgga tcggaggagt cccccccgtg gggtatccgg agcagctccc
    10261 cgccctgctg gacgaagacc tttaccggca cgccgtggtg tgggccgctg cgggcacgga
    10321 ccacgccttc ttccccgtgc cccccggaga attggaacgc ctcaccggcg gccagcgggc
    10381 ggacatccgc aaagagaacg tctaaaagat ccccttcgcc ccgcacaagg gcttgcgcag
    10441 ggggtggagg gctccctccg ggaagcgttt cgtcccccga aggttttttt gcccttaccc
    10501 cgtcggtcat gaggaggttt ttgccatgga ctggggcctt gccctggcgt tgggacttag
    10561 cctttccctg gacgcctttg ccgtctcctg cgccatcccc ttcgccgtgc cccagatttc
    10621 ctggccccag gcgctgcggg tggcggggac ctttgggatc tttcaggccc tcatgcccct
    10681 ggcggggtgg atggcggcgg atctggcggc ggacctcatc gcccccgtgg atcactgggt
    10741 ggcctttgga cttctatccc tggtgggggg acgcatgatc ggggagggat gggccaagat
    10801 gcggtccccc ggccgatgtc agaccctctc gggggacccc acccggggta ggcgccttct
    10861 agccctggcg gtgggcacga gcatcgatgc cctggcggtg ggggtgagct tcatcgggct
    10921 ccgcatcgcc gtgtaccgca cggtgaccgt catcggcatg gtgacctttc tcacctgcat
    10981 ggcggggctc ctggcctcga agcgcctatg cggcgaagga ggaggacgct gggagatcgt
    11041 cggaggaggg gtactggtgg ccatgggcat caagatcctc ggggatcacc tggggttctt
    11101 ctaggaaatt ccataaaaaa gcatgagaag cgacgtaagg gagttgatgg agtgcgaagg
    11161 ctgggacggg cgacgacgat gtggggaacg atcctcggat ttttggtggc ggtgggcgtc
    11221 gcggggggcc cggcggaggc ccaggggatc ccgatccatc gggaccgagg catggaagtc
    11281 tttctggtgg cttgcgacgg aagcaccgat ccggcgagcg ccactgtgct ggtcgatctg
    11341 aaaagcgacg aactgcggcg cgaggcggcg gcccagctgg aggccctgga ccgcgccccc
    11401 gaggaggcct cgacggtggc gcggcgcgtg accaccttcc tctttggcgc cgagggaaag
    11461 cgatggcgga ttcagcggga gaccttcctg ggcgatgggg gaaagaaact ggccgagacg
    11521 gaagatcggg gggagggccc ctgggccgac atgaaacccg gaagccttcc cgaccagatc
    11581 tggcgcgcgg tggaaaccat gaaaaaaaat ggagtaaaat gggccgtccc cccggcactc
    11641 tacctgccct ccggggagat cccgggccga agcccttccc aagacgacgc cccctcggag
    11701 gtggcctacc gggacgaccg ggtatacccc cttttccggg agggggggag ttacgtctgg
    11761 gtcgagctgg actggtcgga gtgcctcatg cgccagagcg attctccggc ccccagagca
    11821 ctggaagcca tggagagggt gcgatccttt gccctggcga ggcacctgga agacggtcgg
    11881 tggcgggtga ccctccaggg tgaaacccgg gataggacca tcttcctccc cgacgaaggg
    11941 gcctttttgg ccctggaggg ctttctgcgg gaggggctgg aaaatcgccg ggtgcgccgg
    12001 gcccatggcg attcctccca agcccctctg gcggcggggg aagggggcga agggcctgcg
    12061 gagatggtcc tctgcggggt ctcgggggac atgaacgaag aagaactgct ggaagtgcgc
    12121 tggctcgggg ccccgggaaa gggaacggtg gtggggacgg ccgaggcgtg gcgttacctg
    12181 gcggatcacc tattcctcgc ccgccgccgg tgggaacatc agcaaagagg gatctacgac
    12241 ttcgtgggaa agctggaaag actggggatc gggaaagctc cgtccccccg gccgtaggaa
    12301 aaaatcccac cgctggcgaa ggttcccgag ggatcgaaac ctcggtgctc tccggaagag
    12361 agggtcggac ttgcggcctt gaggtgagct gtgataaggt atcccgtggt gggcggagga
    12421 cgacgcctcc gcaatctaca aaagaggagg aatagaaaca tggctaaggc tgtggtggat
    12481 cgcgacacct gcgtggggtg cgaggcctgt gtgggggctt gtcccgtgag tgccatttcg
    12541 atgcaggatg gcaaggctct ggtggatgcg ggctcctgcg tggagtgcgg cgcctgcgta
    12601 tcgacctgcc ccgtgagtgc catttcccag taggggcgtc agacttttga agaggagcca
    12661 aaggcgaggg ggctggtgat cccctcgcct ttggtgcttg ccctcgaagg ggcggaagtc
    12721 cggtacgaca gggtgaaagc ggggcgacgg cgtggtgtgg atgcgacaga aggcttggtg
    12781 gaacaaggcg acggggcccg gggggaaggc ggagacggcg gagtccggcg acaactccgg
    12841 actgcccctg tctcgcccct tggactatca aaagctggag aacgcgttgc ggcatctgcg
    12901 atgggcccgc atgatgctgc acgaaatgtc gggggcccac aacggtgaga agcttggttg
    12961 ggagctctac gacatggccc agcgagtgaa cggggacgag agggaagtgg acgccctcct
    13021 aaaaaaattt cgcagcgagg ggattcccgg ggcctaaagt ttcccttgcc ggaggacgta
    13081 aggcggccgg gccccgtccc tcccccttcc ctcctccggc gggcccccgg ggatcatcgc
    13141 cggagccccg ccggagacca taatcaggct atggagacct cctggtcccg gtagacgtct
    13201 tggacggcct ggatgagggc cactccctcg gcgaagggct tctggaaggc cttgcgtcct
    13261 aggatgagcc ccatgccccc tccgcgctta ttgatgacgg cggtcttcac tgcgtcggcc
    13321 agatcgttgg ctcccgccgc gccgccggag ctgatgagcc ccgcccgtcc catgtagcag
    13381 ttggccacct ggtatcgcag catatcgatg gggtggtccg aagagagctt ctcgtagacc
    13441 gccttgtggg tcttgccgaa cttgagggcc gggtaccctc cgttattctc cgggagcttc
    13501 tgcttgatga tatccgcctt gatggtaacc cccaggtggt ttccctgccc cgtgaggtcc
    13561 gccgccacgt ggtagtcctt ttccggcgtg ctgaaggcgc tgttgcggag atagcaccag
    13621 aggatggtgg ccatgcccag gtcgtgggcc cgttcgaagg cctcggagat ctccataatc
    13681 tgcctccggc tctccgagct cccgtagtag atcgtggccc ccaccgccac cgcccccatg
    13741 tcccaggcct gctccaccga ggcgaacatg gtctcgtcgt aggtgttggg gtaggtcagc
    13801 agctcgttgt ggttgagctt cacgatgaac gggatcttgt gggcgtactt ccgggctacg
    13861 gcccccagca ctcccagggt gctcgccacg ccgttgcacc ccgcctccag cgccaatcgg
    13921 acgatgtttt ccgggtcgaa gtacatggga ttgggggcga agctggcccc cgcgctgtgc
    13981 tcgatgccct ggtccacggg gaggatggaa atatagcccg tgccccccag acgccccgcg
    14041 ttaaaaagtg tctggagcga ccggagcacg gggacgctcc gatcggtcac ggccatgacc
    14101 cgatccacga aatcgggccc cgggagatgt aaagacgacg acggaatggt accacacgtg
    14161 tgggaaagca ggtattcggc ctcactgcca agaagctgtc gcaccttgtc gaacgattct
    14221 gacatgccaa cccctcctca aggtgtaaga tggtgatgcc tcggaaagcg gaggtaccgc
    14281 ttttctcttc ccctatcata gcgcaacttc gagagaatgc cactcgagat tctccttaaa
    14341 aaggggggta ttttattgaa agaaccgaaa aagatggccg tttttctgcg ggcctgcgtc
    14401 ctaggggttc tcctgggttg ggctttcccg gccctggcgg atggggtctt cggctcccgc
    14461 ctccaggaag atacgacccg gcgcttcgag aattatctca aaggagaaat ccgggcggag
    14521 cgcctccagg tggcgcttcg aggcacggat ggcgacctcc aggcggggcg ggtccccgag
    14581 gtgaggatct tctgctccgg ggccctcctg ggggacctgc gctgcgaccg cctcatggtg
    14641 gtgctacgct ccgtgacctt tcgcccctcg gggcagggcg ttctcctgga aaaccccggg
    14701 gcggcggagg tttccggcac catcttggaa aaagactgcg aacagtcgct aaaaaaatcc
    14761 ttccccgacc tgaaaaacgc cgaagttcgc ctggaaggaa gccgagtggt ggtctcgggg
    14821 gcctatgcca aaaactatgt ggtgcccgtg agggcctttg tcaagctcta cggcaagtac
    14881 gtcatcgacc cccagagcgg cgaagcctcc ctggccttcg aaaaggccac caacgacaac
    14941 ccctttatct ccacggaaga catcgccaag tccctatctc ggaagagcct gcgcctctcc
    15001 ctggcggaat ttttcgtcaa gccctccctt cgggacgtgc gggtggacaa gagaatggtg
    15061 tggttttcca cgaagcccta aggggccagc gcgaggggta ggcgaccccg gcgaaaggcc
    15121 tggcggggcg cgagggatgg acgggaagac gaacgaaaag atcgagcgga agacgaagcg
    15181 gaagacgaaa gggggaatcc tcatgggcgc gatggatcct gcgtcgacgg tcgtgggctt
    15241 tgtggggacg ggagtgatgg gggccagcat ggcccgccac gtcatgaggg cggggtatgc
    15301 ggtgagggtg tacaaccggt cccggtcgaa ggccgaggcc cttttggccg aaggggccac
    15361 ctgggatgac accgccggat ccctggcggc ctccagcgcc gtggtcatca ccatggtggg
    15421 ttatccggcg gacgtggagg ccgtgtactt tgccccggaa ggaatcctgg cgtcggcgaa
    15481 gcccggtacc tatctcgtgg acatgaccac ctcctccccg gagctggcgt ctcgcatcgc
    15541 ccgggccgcc cgggagcgag gccttcatgc cctggacgcc cccgtatccg gcggagaccg
    15601 gggcgcccgg gagggcaccc tctccatcat ggtggggggg gaggccgaag acctggagac
    15661 ccttcgcccc ctcctgggaa ccatgggcaa gcaagtggtt cttcaagggc cggcgggcag
    15721 cggccagcat acgaagatgg ccaaccagat cgccatcgcc gccgggatga tcggcgtgtg
    15781 cgaggccctg gcctacgccc aaagggcctc cctggacccc gatacggtcc tccagagcat
    15841 cgctgggggc gccgcaggaa gctggaccct gtcaaaccta gcccctcgca tgttggcggg
    15901 ggactttcgc ccgggctttt acgtcaagca ctttatcaaa gacatgaata tcgccctgga
    15961 gtccgccgcc gccctgggac tttcgacccc gggcctggcc ctggcccggg aactctacgg
    16021 ccagctggcg gaagcggggg gagaagagca gggcacccag gccctcttcc gcctctttag
    16081 cgaccgaagg gcgtgacgtt ggagtggccc ggagcccttg acggggaaaa gatttcccac
    16141 tattattatc aacaaagacg gtttgcccca ccggatacca aaaagtcgat gacttaccca
    16201 taggagggat gaccaatgcc cgtgcggccg atctatctgg atcactccgc caccactccc
    16261 ctggacgggg tcgtgctgga ggccatgatg ccctatctgc gggaaaattt tggcaatccg
    16321 aacagcatcc acgcctgggg acgacgggcg agacaggcgg tggaagaggc tcgaggggcg
    16381 gtggcttccc tggtgggggc caaggatcga gaggtgtttt tcaccggggg gggcagcgag
    16441 gccgacaacc tggccatcaa ggggacggcc ctggcccgtc agcaaaaggg gcgccacctg
    16501 atcacctcgg ccatcgagca ccacgccgtc ctggacgtct tttcctggct cggcaagcag
    16561 ggctttgatg tgaccgtgct ctccgtggac gagacggggc gggtatcccc caaggatctg
    16621 gaaagggccc ttcgcccgga cacgaccctg gtgtccatca tgtttgccaa caacgaaatc
    16681 ggcacactgc agcccgtggc ggagttgggg gagctctgcc gccagcggga cgtcctcttt
    16741 cacaccgacg cggtgcaagt ggtggggcac ctgcccctgg atttggccac cctccctgtg
    16801 gatctcctga cgatgtccgc ccacaagatg tacggcccca agggcgtggg ggccctcttc
    16861 gtgcgccggg gggtgaggat cgatcccctc gtgcacggcg gaggtcagga gcgcggcctc
    16921 cgatcgggga cggaaaacgt ggcgggcatc gtggggtttg gggccgccgc ccgcctggcc
    16981 cgggagcgtc tggcggcggg ggagcacctg aaagaagcgg agctccggga tcacctcctg
    17041 gagggcctct tggaggtgga cgacgtgctc ctcacgggac accgcaccga tcgcctgccc
    17101 tttcacgcca gcgtctgcgt ccggttcatc gaaggagaag cgcttttgct ccgcctcgat
    17161 gcggcgggca tcggcgccag cagcggctcg gcctgcacct cgggaagcct ggaccccagc
    17221 cacgtgctcc tggccctggg cctggaccat gccaccgccc acggatccct gcgcctgtcg
    17281 ctgggcaaag acacctcgcg ggaggatgtg gatcgcacga gggccgcttt ccgggagatc
    17341 gtagaagtgc ttcgcgccat gtcccccttt ggcaggaaca agtgaccggc gagatcccaa
    17401 ggaggagatc tgtcatgtat agcgaaaaag tagtggagtt cttcatgcac cccagaaacg
    17461 tggggcgcct ggatcccgcc gacggcgtgg gcgaggtggg caatccccgc tgcggcgacg
    17521 tgatgaaaat ctacatacag gtccgggata accgcatcga tgacatccgc ttcgagacct
    17581 tcggctgcgc cgccgccatc gccaccagct ccatgatcac ggagatggtg aagggaaaga
    17641 ccctggagga ggccctggaa gtatccaacg aggacgtgct cgacgccctg ggggggctcc
    17701 ctccggaaaa gatccactgc tccctcctgg ccgaagaggg gatccgggcc gccgtggaag
    17761 actaccgaaa acgccaaagc ccctgatctt tccggggggt agagccacct ttgaaggccg
    17821 gggactccgt cgtctccagg tctttttccc catcgccccc ggcagaaacg ccgggggcga
    17881 tggcgtttcc aaaaaccggg gatcgcggga cttacgatgg gaacggggcg gatttgccct
    17941 aaaagccctt catcgggtaa gatagggggt atatcgagcc gcagaaaggc cccgagggcg
    18001 gcgaagcgga aggccggtgg aaggcattga ccatgtggaa ggaacatccc tggggaactt
    18061 tggccctctt ggcgttggcg ggagcgatgg cctttctcgc ccgattcgaa ccgcccctcc
    18121 ttcgccaggg ggacaggctt tggcaggatt atcacatggc gacacggcct ctctcggagc
    18181 cctctcagcc catcattatc atgtcgttgg agtcctcccc cggggggtct ttcgatgctc
    18241 cggcggtcct gccggagatc ttggggcgcc tgcgcgaagc ccgggccgtg gtgatttccc
    18301 ccggcgtgga gctacggctc gacgcctccg gagaccaaaa gatcgccgaa gcggcccgtc
    18361 gggcggggaa tgtggtgggg ggagtggccc tcggaggggg aggaggcacc ggaggttggg
    18421 tgcgctttgc cgccgtgacg ggggtggagc tccaagaggc cgactccgac ggccgatatc
    18481 gctccttttc tcccttcgcc ctagtggagg ggaagatggt tccttccttg gccttggcgg
    18541 cctaccttca cgtcacccga gacgtgcccc gggtcaaaga atccccctcc ggccttcggg
    18601 tgctggcgct gggagatcgg cgcctgcccc tggacttcca gggacggttg ccggtggcga
    18661 tccccccgga gggggcgggc ccttcttcct tcgtcgtccg cagcaaagac ctttcccttc
    18721 ccgaagacac cctgcgccgg gccatcgtgg tgttggagct cccctcaagg cagcggcgaa
    18781 ccatgcccct tcccgccttc tcccctgaag cccacgcctc cgccgggaac ttcctggcct
    18841 ggagcatcgg caccctcttc gtcccggccc ttccccaatc cgccccctgg atctggccgg
    18901 cggcgctggc gtggggaggg atggccctcg ccgtggccct ggtatggtgg atgcccctcg
    18961 gggccgtgtg ggcgggggtg ctgggggtct tggtgctggt ggtgggatgc gccgccgtgg
    19021 tcttttccgc cacgctgatc tgcctttccc ccctggttcc cctcctggga gtcctgacgg
    19081 cggggtcggt ggcggtgggt cttcgatgga tacgtcaccg ccgggagagg gccctggcca
    19141 tcctggcggg ggatgcggtg cggtcctttc tggaaggcct tccggcggac gacgccacct
    19201 tcgaagagtg cctccggaag ctctggcccg atctggagcg ccttacgggg gtgaggctcc
    19261 tcggcgcttt tttgccccag ggaatcatcg aggaagaaac tctggacgga ggaaccgtgc
    19321 ttgcccggtc gccccacctt tacctggtgc gcaacgacgt ggatccccct cggtaccgtc
    19381 tctatgcccg ccaggacttt tcccttgccg aaggggagcc ccgcttcgtg gccctggggt
    19441 gggatcgtct catgcccccc gagacgctga ggagcctggc cctcatgctc tctctatcgt
    19501 cttggtttta tgcggggacc cgggcctcgg agcgccgcct tttccacttc aggcaggccc
    19561 tggggggtat cctgcgaaaa aaaactcgga acgccccgat gcccctggtc gccgtggtgg
    19621 acgagctggc ccgccagctg cgatggcacg actctcgccg ggaggacctg cacaccctcg
    19681 ccgttctgga agaggagctc accccccatc ccgacgatcc cccggaggtg gaggctcctt
    19741 cggccgtggg ctttctgaaa agttctgaag gaaatcctct ggaggcggtg ctggaggcgg
    19801 cccgcgccgt cgtccgtgaa atgccctcgg cgggatcttc cccgggggcc ctccgggatc
    19861 ggatgcgggg ggccctccgg ggtccgggga tggccgcgtt ttccccggag ctcctcacgg
    19921 cgttggaaga gatggactac gggacggtcc tcacccctcg agatcccctt cctcccccgg
    19981 ggccatagcc tttctcggag agacctccca tcccccgccg tcacgaagat ttttcagggc
    20041 gtggagcacg ttgcttcgga tgtgggggta gcggtcctgc aagagggcca ggagctcctt
    20101 gatccgggcg cgctccgttc ccccggcgta aggacagggg ggagcgacgg ggcgtagccc
    20161 caggcgttgg atctccgccc ggatgtgccc ctccgccacg taaaccaggg ggcgaatgac
    20221 ggtgatcccg cttcggctgt gccacacccg gggggcgaag cactgaaaac gccctccgga
    20281 gaccaggttc aatagggtgg tttccaccac gtcgtccaga tggtgcccca gggccaaagt
    20341 ggtacacccg gcgctggcgg cggcggagca gaggatccct cggcgcatgg tggcgcaaaa
    20401 gctgcatggc gaccgctcct gtctctgccg gatgatcccc aggatatcgt gggatatcag
    20461 ggaaaggggt acccccaggg cctcgcatcg ggcctcgata aaagacgaat ccagggcccc
    20521 catggagggg tccaaggtgc aggcccggag ggtaaagggc acaggactcc gggacctgag
    20581 ggcccccagg gcttccaaaa gtaccaggct gtcctttccc cccgaaagcc ccaccaggat
    20641 cccgcttccc ggggtgatca tgttaaaatc ggccagagct ttacccacca ggtgtcgaaa
    20701 tcgggcgctc ggttccggga aggtctgagg tgtgtcgggc acgatggttc ctccctattc
    20761 tttgggatgt cgccccaggg gcgccatggc gcccttcggg cggccgtgcc gaaagtatac
    20821 ggggaggtcc cggagtctcg caaggagttc gccccttccc gagatggaat gtttggcgcc
    20881 cccggggtcc cgggggcgat gggcgatgga ggatggggtg cggcgatgaa aaaaattcgg
    20941 ctcgtggtgg ctgacggcca ccggctcttt cgggaggggc tctgcaagct cctttccatg
    21001 gagcccgaca tggaagtggt gggggaagcg gaggacggcg acgaggcctt ccggacggtg
    21061 cgagatctgg ggccggacct attcctcttc gacgtggggc tccccggggg caattcggtc
    21121 tctttggtcc gggatctccg gagccagagg gggcttcgtt tcgtggccat cacgtcctac
    21181 ggcgaccaaa cccacctgac ggccctggcc ctggcggggg tgcacggttg cctcgtcaag
    21241 acttcgggcc tggaagagct ccttgccgcc gtgcggatgg tctcccaggg gatcccctac
    21301 gtggaccagc gcactgcggg gcgcatgatc tttccggacc cccggagccc gagcggcaag
    21361 gatgccttcc tggggttgac cgccaaggaa aaagaaaccc tcttctggct ttctcaaggt
    21421 ttttccaacg ccgacatcgc ggaaaggatg gtggtgtccg aaaagaccgt aaaaaatcac
    21481 gtgagccata tgttaaaaaa gctcgacctc cgggaccgaa cccaggcggc cgtgctggcc
    21541 tggcggatgg ggctggccca gctcgccccg gaagtactgg gggtgcggta gtaggcctcc
    21601 gggtggttgg cgccccgggg cgccctcccg gcgcccggaa agacgaaatt cggggggatc
    21661 cccacagagg ggatcccccc gagtcttgcc cccgagaggc gcctttttcg ctattgggac
    21721 gacgccttgg aggctcccga aagggaagcg aaggcggagg ccagggcgga ggcctgggcg
    21781 ttgaccttgg tgagggcctt ttccatggtg gtgaagcggg tccaaagccc ctgttccatg
    21841 agatccatgc ggatctcgta atttttgacg cgcaggtcga tggccgccac ctgattcttc
    21901 aggtagagga tctggctggc cacgcgcccc tggggcgcgg tggtgggtcc cacctgaacc
    21961 tgggtgcttt ttacgaggtt gtccatgtag gcgtcaagct gggtcatagc cttggtcatg
    22021 agctccccca cgtagggagg ggtatccagc acccggtggg tccggtcctg ggggttggtg
    22081 acgtcgatgc cgagtttttt caggaccgcg ttcccgtcgg tgaagctcag ctccttaccc
    22141 atcttggtgc tgcggatcac cagcttgcgg tctatcaccg aggcggaaac gccgatgctc
    22201 tggccgtcct ccgtgcgggc gtcgttgatc ttttgcgcca tgctggtgag ggagtcgtcg
    22261 gtggtgatgt tcactgtggc cgttctcccg tcctgggtaa tggagaaatt gcctccggtg
    22321 aaccctgcca cgatctttcc caaagactcc gtggcgctgg tcttctggtc cgtcatccac
    22381 tcgtccatga agctcatctt ccctggcgtg agggcgtcca tgaaaagctg cgtatcgaac
    22441 tccagcttgc cgctcttgcc gtagttggcc ttctccgtgg cgatccccac ctgggagagg
    22501 gaccgcaggg ggcccgcgat gttcaggggg tcggagatga gctggcgcat ccgggccttg
    22561 gtctgccaca gcagggagtc cccgtggagg aggccgaatt ttttgttgaa gtcgtcgctt
    22621 ttgttggaat ttgattttga agtttttttc gattcttcgc tgatgcgaac gtttatccat
    22681 tccatgacgt cgttgtagag ctccacgaag gactggacgt cggctacggc cttttccgca
    22741 tccgcttcca cggacatggt gacgtgtccc gcccccttga ggttgagggt ggtgttcccc
    22801 accaggtccg tgatggtgtt gctcgcccgg gtcacgggca cgccgttgag aagaagattg
    22861 gcatcggtgg ccttgctgta gctggagccc agattccccg ccaggccgag gctggagagc
    22921 acccccccgg tttcgtcttc cagatagaag ggattggcgg cgtaggtgta ctcccggcca
    22981 aaggtgacgg tgtagctgct ccccgtgggg ggttcccccc cccccaggct ccagtcgatg
    23041 gtccccgtga ccgggtccca gtggtaatcc gtatctgccg tgtaggtggt ggagcccgat
    23101 ttgaccacga tggtcggagg aaagaccccc gacgaggggc gcgtaagcgt gtccgtgcct
    23161 tcggcgctgc ccttggtctt gttgagggtg ctcttgaagg tgatctcgta ggaggcccct
    23221 tcggcggggt gattggcccc ggcgttccag tggatctttc ccgtggcgcc gtcgtaggtg
    23281 aagtccgtgc cggaggtgta ggtggtgctg ccggatttga tctcggcgat gtagtccggg
    23341 tacccccctt cgtaggtcac cttgaaatcg gtgcctgtgg cgggttcgtg cccggcgacc
    23401 caggaaatgg tccctgtgga gttgtcgtaa gtgtagtccg tggtggctgt tttcccctcg
    23461 atggagatgg tggaggggta ctgtcccgag gtcaggggag tgatctggtc cgtgctcccg
    23521 gtgcctctgg tgtacgccac gtccatcttg tgggggagat agtcgtcgcc ccccgtcgcg
    23581 gcgctccagg ttccccgccg catggtggcc cccgtggcgc tggccgatcc caggccggag
    23641 ttggcagagg agaggacaat gcggttattg accagctggg cggagatgtt gaggcggtta
    23701 tttcccgagt ctacggcctg cttgatcttg tagttgatat cccggagggt gtcgctggtc
    23761 gccaccgcga tggtggcccg ctgtcccccc acccgaatgg cgaaagatcc cgatagcccc
    23821 agcgccgtcg tggcgctgga gtagtagttg gaaatgcggc gctccgctgt ggcggtgtta
    23881 aggacgtcca gatcccattc ctggatgacc gcttcgctgg tgggcgtggc ggtgaggatg
    23941 ctctccgcct tggcccccgc aggggatagg acggagaaca cggcggtctt tttgttgtat
    24001 tcgctgggga gcttcaggga agtcaggatt ttttggagct gtttgagccc cccggagagc
    24061 tcctcgtaca ggccgatctt gagctccagc ttctcctttt cctctttcca gggtttttcg
    24121 atcttccggg cattttccat gatcttgtcg atcatgcccc cccagtctat gccgcttccg
    24181 gccccggtga cctgaaacat ggagtttacc atggcagttc ccctccttgg tgcgtgccgt
    24241 ccggtagggc gcctccgggg atcgtcgccc gaaaaaccgc cccaagcctc ccctccaacc
    24301 ccggggagga ctcctccggg cgcccctcgg ggggcgcggc ggaaaaaatc tctggatggg
    24361 gcacggagac gcttcttcac acctccctcg cagtatcggc atcggaggag gctttcttga
    24421 cggcgggatg ccgcctcccc aattctcccg gggagcccac ggcggagcga cggcgagcgg
    24481 gctcctagct ccgtccgccg tcctcgggat ccccgtcgag ccagcgccta agggccaccc
    24541 tgacggagcg ctccatttcc cgggagaagg cctctcccgt cttgcgcagg taccagtgct
    24601 gtcccgggtt ggacgccccc tcccgcagtt ttttcggcaa cagggcgggt tggtgggcca
    24661 tatcctccag atcttcctgg ctccaggggc ggtaccgatc ctgctgcacg aagaacttcc
    24721 gatccagccg ggggcgaagg ttttgccggt accccgccgg ggggcggccg tagcagagca
    24781 tggtgatggg aaaggcccag ggggggaggg aaaagagctc ccggtgctgc tcgtaccgtt
    24841 ccatgatgtc gccgatgtag caggagccga tgccccggct ttccgccatg atgaccgtgt
    24901 tctgggcggc gatgagggcg tcgcagcacg ccaggagcag gtccgactcc tggggtcttc
    24961 ggaaggattc ctctcttcgg gtacagagat cctccacccc cgaagcgacg taaaaatctt
    25021 gccaacgctg aaaatccgcc aaaaagacga gcaccagggg ggcccgggcg ataaagggtt
    25081 ggtggtcgca gctctccgcc aggacgtctt tgatcctctg gtcgcgcacc tccaggatgg
    25141 tgtaaagcat catattcccc gccgtggggg cccgttccgc cgccagcagg atctgttccc
    25201 ggtcctcttc ggagagggga gtggggtcgt actggcgcag cgaagcccgc tgcaagatgg
    25261 tggcttggac gtctttcatg ccgaggctcc cttctatggc cctttttcct gggccctttt
    25321 ctggcccctt cggacgcttt gccggaggcc ggggccacga tgcgatcata ccacgccccg
    25381 ggaaaaactt tttgcccctt atcgtcctag ggacccggag aaggccccgg gatccctccc
    25441 cgagggagcc gatccgcgcc cttttcccag gttccggcgg ttccggcgat cccggcaggg
    25501 gggcaaaagg gctccacacc cctcttcaga cgtggcaaag tgtgatagta tttcctcgcc
    25561 gttttcccca tgcggcctca cacgaaagaa tcgggaggga agagagaaat gggaaagaaa
    25621 aagacccgtc tggattttgt ggacatgaaa aaacgtggag aaaaggtggc ctggatcacg
    25681 gcctatgact ttcccaccgc ctcctttgcc gaggcggcgg ggatggacat gatcctcgtg
    25741 ggggattctc tggggatggt ggtactggga tacgacggca cggtgcccgt caccatggag
    25801 gattgcatcc gccacagcca ggcggtgcgc cggggcgctc cccagacctt ttgcatcggc
    25861 gacatgccct tcctctcata ccagatttcc gacgaagacg cggtgcgcaa cggggggcgc
    25921 ttcctcaagg aggcctccat ggacgcggtg aagctcgaag gggggcgtcg ggtggcgagg
    25981 cgcatccggg ccctgacgga cgcggggatc ctggtcatgg ggcacattgg cctgaccccc
    26041 cagagctcgg gccagctcgg aggcttcaag gcccaggggc gggaccccga gagcgcccgg
    26101 gagctcatcg ccgacgccct ggcggtgcag gaggcggggg cctggtccct cctcctggaa
    26161 gccgtgcctc cggagctcac ggcctttctc gccaaaaagc tcaccatccc cgtgtactcc
    26221 atcggtgccg gggcgccctg cgacggccag ctcctcatct gcggagacat gttggggctc
    26281 ttccaggcct tcaccccgaa attcgtgaaa aaatacgccc agctggcgga gctggagacc
    26341 gaggccttcc gggcctacgt ggaggacgtg aaaagtgggg ccttccccgg cgacgagcac
    26401 gtgtaccaca tcctccccgg gcgggagaag gacttcgaag agctcttccg ggagtttccc
    26461 tgaccgtcct gaccccttcg gggtgacccg gggcgaagag tccgggggcg gggggtgtcc
    26521 cgtcgaactt ccccccgccg agagcgtctt cgcccgagat cgcctttgcc gggagcgccg
    26581 gcttccagcc ggccttttga ttagaggacc tgtgaaggag accgatggcc ttccaatggg
    26641 gcggcatagg ggcgaaaaac atgctcgccc ctacgttttt tcgcccgtgt cgattttcac
    26701 cgctacagag gggatgacgg ggcggagatc ggtgtttcct gccgatggta atgacgtgag
    26761 ccaccatgga gaggtttttt aagagacaaa tgatgccggc tggaagatgg gcgcttccag
    26821 ccatgcgctc ctcagccggc tcgaactggc gtcttcgccc gagatcgcct ttgccgggag
    26881 cgccgcctcc cgagatcgcc ctcgcccgag agcgtccccg cccgagatcg tccccccgtc
    26941 gaccggactt agcccctttc gtcgctcctc tttggatatc tggcggatcg atcccgggga
    27001 agcgttcttt ccgccgcatg ggttcatgcg atatttaacg gggtgcggga ctgggggatg
    27061 gttttcctgg aggttcacgc ccacctttgg ttctagggga gattttcgtc ctctccctgg
    27121 gtacgggagc cgaagcatcg tttcatcccc cccggcgtgc gttatgattg gtgcggccgt
    27181 cggcgaggac ggccggaaaa tcagggtcgt gggggaaacg ccggaaaaaa agtaaggggg
    27241 aggctccgtg aggaagtgga tgatccttgt cggattggtg gcgctgggcg ttctgggcgt
    27301 ggcatggaac tctggaagac tttttcctgg gctggggaaa tccctggaaa ggtctcgcgc
    27361 ggaggagggc gtgacggcct cgggcaccgt ggaaatccgc acggtggagg tggcttccaa
    27421 gctgggagga tacctgcggg agctccgggt ccgagaggga gatctcctgg cggcggggga
    27481 gctcatcggc ggggtggacc gccccgatct ggaggcccag atttcccgag acgaggcgtc
    27541 gctgcggagg gcccgggccc atctggcaga ccttcagtcg gggagcaggc cggaagaaat
    27601 cgccgaggcg gcggcccggg tggcctccgc ccgctcgcgg tacgagaaat cccgtcagga
    27661 cttcaatcgt tttgagagcc tccaccgcga taaggtcatc tcggatcgcc agttcgacga
    27721 gtaccgggcg gccttcgacg gggcgaaggc cgaactggcg gcggcccagg atcgccacgc
    27781 cctcctccgg gcgggggctc gccccctgca ggtggaggct cagcgcgccg aggtggaggc
    27841 cctggaggcg gccctggccc tcacccgaag cctctgggca gactgccaga tccgggcccc
    27901 ccaggggggg gtggtgcttt ccaaaggttt tgaacggggg gagtacgtag cgccgggagg
    27961 agtgctggtg accctgggcg atccgatgga ctgctgggtg cggatgtaca tcccatccgc
    28021 ccaggtgggg cttttgcgcc gggggcagag ggccgaggtg cgggtggatg cctaccccca
    28081 ccgcacgttt tccggccagg tgacggagat cggccaggag gcggagttt
//
LOCUS       DAOOER010000025        26752 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_2095_length_26752_cov_8.952841, whole genome shotgun sequence.
ACCESSION   DAOOER010000025 DAOOER010000000
VERSION     DAOOER010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 26752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26752
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2095_length_26752_cov_8.952841"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            274..576
                     /locus_tag="PL162_04990"
     CDS             274..576
                     /locus_tag="PL162_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91411.1"
                     /translation="METLKTGVWEVATGEVGAPGSGRDAFEATAQELRIRVEDGGPYD
                     KDGRKNASVTLETATVLLGAPREGPSPLPSGSPGSFPLSSLLLLLLVPLGVLKGSK"
     gene            complement(831..3866)
                     /locus_tag="PL162_04995"
     CDS             complement(831..3866)
                     /locus_tag="PL162_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQF91412.1"
                     /translation="MNIASLAFRSKAFVWFLTVILIGAGLMAYGKLGKLEDPAFTIKT
                     AVVMTPYPGASPREVEEEVTEVVESAIQQMGQVDKVRSLSQAGMSMIYVDIKDHFTAP
                     DLPQIWNELRQKVGDMQRSLPPGAGPSLVNDDYGDVFGIYYALTGPGYTLRELGSFAD
                     DLRKELLLVPGVARVQIGHRRQEAVYVEISRTKLAQLGISLQEIAGAIQNENLVVSSG
                     MVEVGKEYLRIDPTGIFTAVEQIGDLILRSSGGTLIRLRDVASVRRKYVPHPSSLMFY
                     NGTPAMDIGISCVEGGNVILLGEEVEKRLQSFASHTPLGMELHPIYFQPGTVQTSIDV
                     FMENLAEAVAIVVALLLVFMGLRSGLLIGAILVLTILATFVVMKAFAIDLHSISLGAM
                     VIALGMLVDAAIVVVDGILIGSQMGKTREDAAKGIVGQTQWPLLGATVIAIIAFAPVG
                     LSPDSTGEYCKSLFQVVGISLLLSWVLAVTITPLAGAAFLRTPPLPEGENPFGSPTYQ
                     KYRELLEFCLTHRPITLIALGGMLALAAVGFTHVAPGFFPSDTSPMFTVDFWRPRGSS
                     LAQTRQDAERMEAFLLRQEEAPSVTSYVGEGAMRFLLTFTPADPGNHYGSFVVSTHTG
                     EEGTSLMKKITDFAAQEMPGLDPRVRSFGKGTGSGAKIQVRVQGEDPDALRKTAERIS
                     AILLSDSRAINVRSDWGERTKVLRPVLDEVRARTSGLTRRDVAQALEMAFSGTTTGIY
                     RDGDKLLPIVARLPREDRSDAGTLPTVSVWSAALRTYLPLSQVVRAFDNVAEDQTIYR
                     RNRVRTITVECDAGDGQTAALFDTLRPQVEALPLPSGQSLEWGGEFESSTRAKAGLMG
                     MIPLGFLFIVIILVALFNSYRQPILILLTLPLSIIGVTAGLLMFNKPFDFLALLGFLS
                     LAGMLIRNAIVLIDQIDLEIHEGKPPFQAVVESAMSRARPVLMGAFTTVLGMVPLYFD
                     VLYSAMAVVIMFGLTFATVLTLVVVPVFYAVIFRIPAKS"
     gene            complement(3875..5176)
                     /locus_tag="PL162_05000"
     CDS             complement(3875..5176)
                     /locus_tag="PL162_05000"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQF91413.1"
                     /translation="MAERQRNRRSWWWGVAALVALGGGFLFYRGKDPAPLKPAESLRP
                     VRPVKTMELMPPVNALERSYPGKVQSSHRVELAFRVSGPLVELPVKRAQAVHRGELLA
                     RIDSRDYVSQLANAESALANAQAQLAAMKSGARKEDVAASAASVSSAKAHLAEAEAQY
                     RRFEKLHQQGAVADAEFDRYKANYEVAKAQYNAAGQELSKARTGARPEDVKAMEATIA
                     GLAAKVSQARSSLEDTELRAPFDGFIADRHVENFQSVQRDQPIVNLQDLAHLEVTVAV
                     PEQDLLLFMGVSRDMVARAFSEVTGSKEIPLTFKEIATQAESQTQTYAATFQIVSREG
                     INLLPGMTLSVRIGGRARGAELSGPFRVPSEAVMAGEGTRHSVWVIQENLSVRRVTVA
                     LLSFREGDALISGDLKAGDRVVTAGANLLLDGETVSLYTPK"
     gene            5398..6051
                     /locus_tag="PL162_05005"
     CDS             5398..6051
                     /locus_tag="PL162_05005"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HQF91414.1"
                     /translation="MELLRFLFREALLSMDYPASADIFSRAKDAVGSPEVAAEELLLP
                     VLEKFGEEWEAGQLSLAQVYVAGRICDDLTAPLAGPQEAAVDLPVAMAGLRDHHALGR
                     RIVLAHLRIGGIAVRDYGSGVTPEEVVDRAAQDQIQVLLLSALMLASALDVRRVRQLC
                     QERNFSPALVVGGAPFRLDPGLGRAVGADAVGYTASDARKHVRHFLKERGIGSWGVS"
     gene            6036..7097
                     /locus_tag="PL162_05010"
     CDS             6036..7097
                     /locus_tag="PL162_05010"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HQF91415.1"
                     /translation="MGSVMTSMERVGAALSHRKPDRVPLVLLFGYAAAREAGVSVQEY
                     MAREDLQVETQKAVRGRYALDCLSAFTYLGAEVEAMGSEIIFSPEAPPQVGKPIFSPP
                     EALRAFEPPRPEDAPVLARTLRVLVALRRDSGDEAPIIGVVASPFSIPLMQLGFEGYL
                     KLLAADRQGSQAAGELLDLLWKKNSHFAEIWGAAQLRAGAHALCYFDPLSSPSMVTRE
                     MWVRGGRPAAERFLAAFPGVPVICHLASGRGEGVIKDLADLGFVAVGVSAEEDLGELK
                     AVADRRIALVGNLNGIAMRHWSAEETEAQVRLAVAKGAPGGGFILSDNHGEVPWQVPP
                     EVIRGLGDALARHGTYSPD"
     gene            7110..8747
                     /locus_tag="PL162_05015"
     CDS             7110..8747
                     /locus_tag="PL162_05015"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQF91416.1"
                     /translation="MTMILRVVVCRALLREVEALVEGEAVWVEGVPEACSPGDSPPEM
                     APGDRGHPTIFLGCEAHRFPKNDPWNRRVGGSHCASLLGGAWLTAHIAASRGYAVTPS
                     WVEDWPRRLERWGFDWPLGREFFHGFADRLVLLDSRTFPEAPERLRECAAALGLGHEI
                     LPVGLEPLRLAFREARSSLELEGAEGRVRELRKEAGEAFMAMDLMGQVAELGKEDQVA
                     SRLHDLCSLLFDPRSVAYAPRGKGAPKKVWYFPSSAEEGEEPPAEGVAPGGVVREEGG
                     FSLGVAWGGETLGVLRVRGVAVPKHLDRYADLTSRVISRVAGLAVANARSFETLEKTL
                     QDREFLIKEIHHRVKNNLNLVVSLLSLQDPGEQDGALHKALNEAISRVHAIAQIHDFL
                     YRGEDLRSVPLGPYLKALGAHLREFGAEANNSVTLVISGDEGLSLEPKRAVSCGLMVT
                     ELVTNALKYAFPPGGPHPRSPEVRVTAERREGGMVALEVRDNGVAKDQAPETPKGSSL
                     GTLLVNALTQQLEGTVVRSHTEKGYTVTLLFPEKEEA"
     gene            complement(8811..9518)
                     /locus_tag="PL162_05020"
     CDS             complement(8811..9518)
                     /locus_tag="PL162_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQF91417.1"
                     /translation="MTPRGLRIQGLKKRYGSGDAAVDALKGVDMTVAPGEVVGLIGPS
                     GSGKSTLLKCLGAVIEPTAGKMALGEDVIYDGEWKVKDLRALRRDRIGFVFQAPYLIP
                     FLDVTDNVALLPMLAGVPNAEARHRATELLRALDVGHRAKAMPSQLSGGEQQRVAIAR
                     GLVNRPPIILADEPTAPLDSDRALAVIRILNDMARQFDTAIVVVTHDEKIIPTFKRIY
                     HIRDGVTYEEAGQRRTP"
     gene            complement(9520..10725)
                     /locus_tag="PL162_05025"
     CDS             complement(9520..10725)
                     /locus_tag="PL162_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011734096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQF91418.1"
                     /translation="MISLAGRDIMHSWGKFVFTGLGLGLLIGITLSMAGIYRGMVDDA
                     KVLLDGSGADLWVVQKDTLGPYAEPSSIYDDVWRGLRGMPGVEEAANITYLTMPVGTR
                     DGAVRAMVTGIAPGGPGTPGWPPHLVAGRPITRGHYEAVADVASGLTLGESIQIRRHT
                     YTVVGLTRRMVSSGGDPMVFIPLKDAQEAQFLKDNDAIREQRRRTAENPAFQRPGVPG
                     LLEAISASQGANPYVNAVLVRVKAGHDPEEVAAAIRRWKRLTVYTRYQMEDILIGKLI
                     ATSSRQIFLFLVILSVVSSAIVAFIIYTLTMGKIREIAVLKLIGTKNHTIVGLIMKQA
                     LALGLIGFVVGKISATLLMAPIFPKYVLLLPLDSALCLAIVMAICLLSSTVAIRIALL
                     VDPAEAIGG"
     gene            complement(10722..11873)
                     /locus_tag="PL162_05030"
     CDS             complement(10722..11873)
                     /locus_tag="PL162_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQF91419.1"
                     /translation="MKKMTFGKRLLALAVVLSPLMALFVYVALRSGPLAPVAVVLAPV
                     KIKALSPAIFGIGTVEARHIYKMGPTLAGRVKSLDVQVGDRVQAGQRLGEMEAVDLDE
                     RLKAQDAAIKQAQAKVNEAQTYKDHAQTQASRYDKLLKDHSTSEEVASAKRQDLSMAQ
                     ARLAAAREELARARAEREALEAQRNHLALVAPVNGLVVRRDADPGTTVVAGQAVVELI
                     DPDTLWVDVRFDQIRSGGLDAGLPAQITLRSRAENPQTGHVLRVDPLADPVTEETMAK
                     VVFDRLPDPRPPVGELVEVTVALPAVAAAPVIPQAALHRVEGRWGVWRVVSDDLQFVP
                     VSPGIADLEGLMQVREGLEEGDQVVVYSESALTPHRSIRIVDHIKGVTR"
     gene            complement(11870..12478)
                     /locus_tag="PL162_05035"
     CDS             complement(11870..12478)
                     /locus_tag="PL162_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQF91420.1"
                     /translation="MNQTKKNLPAEKRRALTVETMISLAGEQNPSEITTAAIAQRMGL
                     TQGAIFRHFPSKDAILQAAMDWVAQRLISRSEEATSAACSPLASLEGLFLFHAEFIAS
                     HPGIPRMLFGELQHPEDRIPKRMAQAIMARYKESLRTLLHRGRDCGELDEGLDVEAAA
                     TLFIGALQGLVVQSMIAGDGSRVRHDAPLIFALYRRGLRRMA"
     gene            12983..14179
                     /gene="trpB"
                     /locus_tag="PL162_05040"
     CDS             12983..14179
                     /gene="trpB"
                     /locus_tag="PL162_05040"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HQF91421.1"
                     /translation="MTSTAKASQKGFFGEYGGSFVPPGLQPRLDELERAYEEALRDPA
                     FAEELTELLRDYVGRPSALTFCGNISRKYGGGRLFLKREDLNHTGAHKINNALGQALL
                     AKRMGRKELIAETGAGMHGTASATVAALMGMKCTVYMGAVDVDRQAPNVARMKALGAK
                     VVAVQEGQKTLKEAVDVALRAYVERPESFYLLGSAVGPHPYPVMVRSFQSVIGREARR
                     QFLEREGRLPDYAVACVGGGSNAIGLFAAFLDDAAVRIVGVEPAGRGLTPGDHAATLT
                     MGSPGVIHGFRSYVLTDGQGEPAPVYSISAGLDYPGVGPEHAFLKDSRRGEYVTVTDQ
                     EAVGAFQTLCHLEGIIPALESSHALAYALKLLPTLSPEKTVLVNLSGRGDKDMATILP
                     LLDLAD"
     gene            14518..16161
                     /locus_tag="PL162_05045"
     CDS             14518..16161
                     /locus_tag="PL162_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HQF91422.1"
                     /translation="MGFYVDVVGWRRISSEIPLSGPDVLRELGIEGRREVVAWRVNRY
                     LRSLDWIIDEDATVELVDASSFEGMEVYRRTLSFLLVLACRQALGCQISIRHSISNAY
                     YCELSCGPASADQVARIRQALRDLVDQNIPIRRSVVSLDKARRIFEAQGNDDKARLLQ
                     WAVQDPVELYSCQGRFGFYYAPLAPSSGAVPLFDLISYAPGMVLRFPTIHSPFSLPDF
                     QATPRLVDVFREYGEWLDILDVGTMASLHEQVAEDKTVDIIMVSEAFHARRLALVSEE
                     IVRRKSVRLVCMAGPSASGKTTASRRLRVQLQLSGLHPVALSLDEYFVDRERTPRDEE
                     GQYDFEDINALDLELLNSHLQRLLAGDEVQLPRFDFLLGKRTPGRKLRLRPKDILIIE
                     GIHGLNDRLTEAVPEDLKYGIFVSPLTGVSLDRHTRTSTTDNRFLRRMVRDHRTRGKS
                     PENTILQWPSVTRGAFKHIFPFQDRADALFNTVLVYELSVLRGYAEPLLRTVPESSLA
                     FGEAQRLLSMLRFVPVIPADNVPNTSILREFIGGSCFGD"
     gene            16279..17592
                     /locus_tag="PL162_05050"
     CDS             16279..17592
                     /locus_tag="PL162_05050"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HQF91423.1"
                     /translation="MAERIAVIGTGYVGLVAAVGLADFGNSVVGVDVDPEKVAKLQGG
                     VSPIYEQAMEEYLERNLRNGRLSFSTDVEAAVRSSRLVFIAVGTPEGPGGDPDLSQVE
                     AVARLIGRCCADYKVVVIKSTVPVGTNRRIARLLRDLAAPVEVDVVSNPEFLREGRAV
                     YDFFNPERVVIGGESPRATAILRSVYRSRHLDETPFLFCSWETAELIKYASNGFLATK
                     ITFMNQMSQLAECVGADVATLAKGLGMDSRIGPLFLQAGPGYGGSCFPKDTIALVRTG
                     ERCGADMTLIREVIRANEAHKARMAEKVERLVGPLVGRTVALLGLAFKAGTDDVRESP
                     ALVIAEELLRRGARVRVHDPKALENARKILGDRVAYCASEFEALTGAHAVVLATEWNE
                     YRNLDLERARGLMAALALVDLRNLVEPSEARARGFAYDGVGYPGP"
     gene            complement(17840..18757)
                     /locus_tag="PL162_05055"
     CDS             complement(17840..18757)
                     /locus_tag="PL162_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQF91424.1"
                     /translation="MPTLCVKIDVDTLSGHREGVPALLRLFDRLELRASFFFSLGPDN
                     SGKALRRIFRPGFLAKMRRTKAVSTYGWRTVLSGTLLPAPLIVPSAPEPLRDAVTSGH
                     DCGVHAWDHVAWQDTLLSTSPVRLREDFDRALELFARVAGREAQSCAAPGWQTSAESL
                     MMQERYRFAYCSDVRTGEPFLPQVGTAVLRTPQMPTTLPTLDEIYGAPGLSEEGVIDL
                     YLDHLSRGNLHIHTVHAEMEGGAHLPLLEELLRRARDRGFVFCTLGDVAATLDRRTLP
                     VYPVVQRPIEGRAGTVSMPWRSPRRAGEF"
     gene            complement(18739..19770)
                     /locus_tag="PL162_05060"
     CDS             complement(18739..19770)
                     /locus_tag="PL162_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-keto-pentose/UDP-xylose
                     synthase"
                     /protein_id="HQF91425.1"
                     /translation="MKILILGVNGFIGSHLSERLLADPQWEVTGFDLAGDYVARWAHH
                     PRFTFRQGDIFREDAWLAEAVAASDVVFPLAGVAKPALYVKRPLYVYELDYEQNVKIA
                     RLCAEHGTRIIFPSTSEVYGMSPHRELKEDESPLVLGPIAASRWIYSCSKQMMDRVLA
                     ALGQERGLRYSCFRPFNWMGPRLDTFDDAARHEARAVTQMLYDMIHGRPVVLVNGGAQ
                     RRSFTHVDDGIDGLLAILTQEERAQGQIFNLGNPDNNASIRELALAVRDALKEHPAWR
                     AVAEAAVLEDRPAAAYYGPGYEDVDDRRPSVAKARELLGWVPRIGFTETVRRTVASIL
                     ETTPCPPSA"
     gene            complement(19866..20474)
                     /locus_tag="PL162_05065"
     CDS             complement(19866..20474)
                     /locus_tag="PL162_05065"
                     /inference="COORDINATES: protein motif:HMM:NF025468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HQF91426.1"
                     /translation="MVVKLYVEGGGDSERLHRRCREGFRVFLERAGLKGQMPRIVACG
                     GRQAAYERFRVACDAHQETALLLIDSEEKVSGHSPWEHLRREGFFPPCHTNDDHCHLM
                     VVCMESWLLADHETLSRFFGPGFNAGALPQRGNVERLSKKEVYESLRKASAHCKTKAP
                     YDKGEHSFPLLLQIDPEKVSSASPWGARFLHVLRNIAKKDGG"
     gene            complement(20468..21655)
                     /locus_tag="PL162_05070"
     CDS             complement(20468..21655)
                     /locus_tag="PL162_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQF91427.1"
                     /translation="MITSLRLKNFLSYGPEGETVPLGALNVLIGPNGSGKSNLIEGIA
                     LLQSAPSRLDTPIREGGGVRDWLWKGTKEIPTATIEALVELFPGTEGKLPLRYRLDFT
                     ASGGRFEITDERIESTAPCSGHDGPFFYYRYEEGHPVLSVRDPEDEKRWWRTLRREDV
                     DPSRSILAQRQDPDIYPEITRLGEVFGRMRIYRDWSLGRYTPPRLPQPADLPNGWLEE
                     NGRNLGLVLNRLRRDSGAKSRLLEMFRAVYAEAEDFDVMVEGGTVQVFVQEGRFSVPA
                     TRLSDGTLRYLCLLAVLCDPTPPPLVCIDEPEIGLHPDLINHVADALRLAAERTQIVV
                     TTHSTGLVDAFTNTPEVVLVCEKENFHTSVHRLDEGALRPWLQQYGLGHLWTSGEIGG
                     NRW"
     gene            complement(21744..22694)
                     /locus_tag="PL162_05075"
     CDS             complement(21744..22694)
                     /locus_tag="PL162_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase"
                     /protein_id="HQF91428.1"
                     /translation="MAEVRSFPPAGRRPRVVLLAYSEVGVRCLETLCALGASVVAVFT
                     YRDDPHEEIWFRSVADGARAAGIPVLFESLRSPEGRELLQGLAPDLLLSAYYRDMIAP
                     EVLALPPGGAFNVHGSLLPRYRGRACINWAILRGETETGATLHEMVARPDAGDVLDQE
                     GVSITFEDTALSVSLKVADAAARILARTWPLVEGGTAPRFPQDASRASSFGRRGPEDG
                     RMHWELSARELYDLVRAVTHPWPGAFFPLGEKKILVWRAFPEEGEEISPGEVLSVAPL
                     RVGTGRGVLRLDRVQEEGDEERDGNVWGATHLHTGERLDA"
     gene            complement(22687..23682)
                     /locus_tag="PL162_05080"
     CDS             complement(22687..23682)
                     /locus_tag="PL162_05080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQF91429.1"
                     /translation="MSTEPRDPASAPLLSVVIPVYNEEESLPRLFPRLMPVLEALQKP
                     YEVIFVNDGSRDGSLGLLLAHRDRFPHCVRVLDFNGNFGQHMAILAAFENSRGRYLIT
                     MDADLQNPPEEIPRILAELEAGHDVVGTYRENRHDPLFRKVASRIVNKFTNRITGLGL
                     KDYGCMLRGYDRRVVDYINQCQETTTFLPALARKFATHPVEIAVGHAEREQGASKYGL
                     YRLIRLNFDLMTGFSLVPLQAVTMLGTLVSFLSSVFAVFLLLRRLVLGPEVEGVFTLL
                     ALNFFLMGITLLSIGIAGEYVGRIYLEVRRRPRFLIRKIYDDAKDPLSPGGDRRG"
     gene            complement(24126..25769)
                     /locus_tag="PL162_05085"
     CDS             complement(24126..25769)
                     /locus_tag="PL162_05085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQF91430.1"
                     /translation="MRLPDEKAFLPRLGILLLTLGVLYFWKLGSHGLLEPDEGRYGEI
                     PREMIETGDWTTPRLNYVKYFEKPVLHYWLTAGAFRLLGETEFASRVVPALASLGGAG
                     AVALFGASLWGPRGGIAAGAVLGSSLLWFASAHINITDMTLTFFLTLSLLGFHRAMTG
                     SRGWLALFYGGAALATLAKGLIGVVLPGAVIFWWIVLTRRWEYVRRGFSFAGVLFFFT
                     LASPWFLAVIRANPEFFWFFFVHEHFLRYTTMVHGRFEPWWFFLPILAVGFLPWTGMA
                     LAALGKALKGEGEGRADGENLLFMLLWFAVILVFFSLSRSKLVPYILPAMPPLALLTG
                     WALAQPPALGRRRSLLWGVGLSTLFMLPLALALGVYPFFQDKYDPSRLLALGLPWGGV
                     LLGGVLLLWWALVRRPWALGGILLGVALVQGAAMKPVFSFYGEIISARGLASTVAAHR
                     QPGDGVANVGEYHQGLPFYLKQRVLLVNYYGELAFGASQDRAGEWFISSADFRPRWEG
                     NGGMILVAPREAYEDLVRGGFPPGRFLGEDQGQVVVRNR"
     gene            complement(25756..26478)
                     /locus_tag="PL162_05090"
     CDS             complement(25756..26478)
                     /locus_tag="PL162_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HQF91431.1"
                     /translation="MGNETIFFVTVLANFGGILLAYRLWGKTGLFCWVALASVIANIE
                     VVKTVSLFGLVATLGNVIYGTTFLATDILSENHGRQDARTAVGIGFFSLVVFTVLMRL
                     TLLFRPDASDFASPALETIFTLLPRLAVASLLAYLVSQFHDVEAYHFWWRRFPGVRFL
                     WLRNNGSTLVSQALDTLIFVGVAFWGVFPPEELGQIFLSTYLIKALVALCDTPFIYLA
                     RRWRDTGKVATEVEGDPSYAPS"
BASE COUNT         4837 a         8401 c         8675 g         4839 t
ORIGIN      
        1 gattcggacg aagggccgcg ccatgaccag gagacttcaa gagaccgaga agacagggaa
       61 gaacgcgcgg aggctttccc ccgagatccg gacctttcgt atcgggggct gcgcaaaaag
      121 ggctacagca aagacgacaa ggtcaacgaa acgtaggtag tcctggccct ggccacctcg
      181 gcggaggggt tggatcgggg gactcctttt ttttctgcaa accttaccct gaaaggccac
      241 cgatgttaag ccgtttccgg ccagatagag ggagtggaaa cactgaagac cggagtgtgg
      301 gaagtggcca ccggagaggt gggagctcct gggagcggcc gagacgcctt tgaggccacg
      361 gcgcaggaac ttcgcattcg ggtggaggat ggagggcctt acgacaagga tggaaggaaa
      421 aacgcctccg tcactctgga aacggccacg gttcttttgg gagctccccg ggaagggcct
      481 tcgcccctgc cgtcgggaag ccccgggagc tttcccctgt cgtcgctgct tttgctcctg
      541 ctcgttcccc tgggggtgct taagggatcg aagtagcatc gtgggggcgg ggcgttttcc
      601 cgcccccatt ttttttcggg aatcccccgt ccccgggagt tggcagttcc ccttgcggga
      661 acgtcaaagg ggcacccccc aggggtgccc cttttcggtc cgtgttctcg tgtcccagga
      721 gtttcccagt gggatcggga tctgtgctca ccgcattttt ccgatcttct cagagttttt
      781 cccagaagag ccttgggggg gagatcccgt cccgtcggtc tgacgccgga tcagctcttt
      841 gccggaatgc gaaagatcac cgcgtagaat acggggacca ccaccagggt gagcaccgtg
      901 gcgaaggtga ggccgaacat gatgaccacc gccatggcgg agtagagcac gtcgaagtag
      961 aggggcacca tgcccagcac tgtggtgaag gctcccatga ggacggggcg ggcccggctc
     1021 atggcggatt ccaccaccgc ctggaagggg ggcttccctt cgtggatttc caggtctatc
     1081 tggtcgatga gcacgatggc gtttcggatg agcatccccg ccaggctcag gaaacccaaa
     1141 agcgccagaa agtcgaaggg cttgttgaac atcaggagcc ccgccgtgac gccgatgatg
     1201 gacaggggca acgtgagcag gatgaggatg ggttggcggt aagagttgaa aagtgccacg
     1261 aggatgatca cgatgaacag aaaccccaga ggaatcatcc ccatgagccc cgctttggcc
     1321 cgggtggagc tttcaaattc tcccccccac tccaggctct gccctgaagg caagggcagg
     1381 gcctccacct ggggacgcag ggtgtcgaaa agggcggcgg tttggccgtc ccccgcatcg
     1441 cactccacgg tgatggtgcg gacccggttt cgtcggtaga tggtctgatc ttccgccacg
     1501 ttgtcgaagg cccttaccac ctgggacagg ggaagatagg tccgcagggc ggcgctccac
     1561 accgagaccg taggcaacgt ccccgcatcg gaacgatctt cccggggaag gcgggccacg
     1621 atgggcaaga gcttgtcccc atcccgatag atcccggtgg tggttcccga aaaggccatt
     1681 tccagggcct gggccacatc ccgtcgggtg aggccgctgg tgcgggcccg cacctcgtcc
     1741 agtacggggc gcaacacctt cgtgcgctcc ccccaatcgg aacggacgtt gatggcgcgg
     1801 gagtcggaga ggaggatcgc cgaaatccgc tccgccgttt tcctcagggc gtcgggatcc
     1861 tctccctgga ctcgaacttg gatcttggcg ccgctaccgg tgcccttgcc gaaggatctc
     1921 actcggggat ccaggccggg catctcctgg gcggcgaagt cggtgatttt cttcatgagg
     1981 ctggttccct cttccccggt atgggtgctc accacgaagc ttccgtagtg gtttccaggg
     2041 tcggcggggg tgaaggtgag caggaagcgc atggcgccct ctcccacgta gctcgtcacc
     2101 gagggggctt cctcttggcg gagcaggaag gcctccatgc gctctgcgtc ctgacgcgtc
     2161 tgggccaggg aggacccccg ggggcgccag aagtccacgg tgaacatggg gctcgtgtcg
     2221 ctggggaaaa agcccggggc cacgtgggtg aaccccaccg ccgccagggc cagcataccc
     2281 cccagggcga tcagggtgat ggggcgatgg gtcaggcaga actccagcaa ctctcggtac
     2341 ttttggtaag tgggagagcc aaaggggttt tccccttcgg gaaggggagg ggtccggagg
     2401 aaggccgccc ccgccagagg ggtgatggtg accgccagca cccagctcaa aagcagggag
     2461 atccccacca cctggaagag gcttttgcag tattcccccg tgctatcggg ggagagcccc
     2521 accggggcga aggcgatgat ggcgatgacc gtggcgccca ggagaggcca ctgggtctgc
     2581 cccacgatgc cctttgccgc atcctctcga gtctttccca tctgagatcc gataagaatg
     2641 ccgtccacca ccacgatggc ggcgtccacc agcatcccca gggcgatgac catggctccc
     2701 agggagatgc tgtggaggtc gatggcaaag gctttcatga ccacgaaggt ggccaggatg
     2761 gtgagtacca ggatggctcc aatgaggagg ccgcttcgaa gtcccatgaa gaccaggagg
     2821 agcgccacca cgatggccac ggcctccgcc aggttctcca tgaagacgtc gatggaggtc
     2881 tggaccgtgc cgggctggaa gtagatgggg tggagctcca tccccagggg agtgtgggag
     2941 gcgaaagatt gcaggcgttt ttccacctct tctcccagga ggatgacgtt gcccccctcc
     3001 acgcaggaaa tgcctatgtc catggcgggg gtgccgttgt aaaacatgag gcttgagggg
     3061 tgggggacat atttccggcg caccgaggcc acgtcccgga ggcggatcag cgttcctccg
     3121 ctgcttctca ggatgagatc tccgatctgc tccacggcgg tgaagatccc cgtgggatcg
     3181 atgcgcaggt attccttgcc tacctccacc atgccggaag acaccacgag gttttcgttc
     3241 tggatggccc ccgcgatttc ctggagggag atgccaagct gggccagctt ggtgcgtgag
     3301 atctctacgt agaccgcctc ttgccgtcgg tggccgatct gcacccgggc cacccctggc
     3361 accagtagga gctcttttcg gaggtcgtcg gcgaagctcc ccagctcccg gagagtgtat
     3421 cccggccccg tgagggcgta gtagatgcca aagacgtcgc cgtagtcgtc gtttaccagc
     3481 gatggccccg ctccgggggg gagggagcgc tgcatgtctc ccaccttttg ccgcagctcg
     3541 ttccagatct ggggcagatc cggggcggtg aagtggtctt tgatgtccac gtagatcatg
     3601 gacatccccg cctgggacag ggagcgcact ttgtccacct gtcccatctg ctggatggcg
     3661 gattccacca cttcggtgac ctcttcttcc acctctcggg gggaggcgcc ggggtacggg
     3721 gtcatgacca cggcggtctt gatggtgaag gcggggtctt ccagcttgcc cagtttgcca
     3781 taggccatga gccccgcacc gatgaggatg acggtgagga accagacgaa agccttgctg
     3841 cggaaggcca gggaggcgat attcatggtc tgacctactt cggggtgtac agggagaccg
     3901 tctccccatc cagaagaagg ttcgcccccg ccgtcaccac ccggtctccg gccttaaggt
     3961 caccggagat gagggcgtct ccctctcgga aggagaggag tgccacggtg acccggcgta
     4021 ccgataggtt ttcctggatg acccacaccg agtgccgggt tccttctccc gccatcaccg
     4081 cctccgaggg cacccggaag gggccggaaa gctccgcgcc ccgggcccga cccccaattc
     4141 ggacggagag cgtcatgccc ggcagcaggt ttatcccttc ccgggagacg atctgaaacg
     4201 tggcggcata ggtctgggtt tgggattccg cctgggtggc gatctctttg aacgtcaggg
     4261 gaatctcttt ggatccggtg acttcggaga aggcccgggc caccatgtcc cgggagaccc
     4321 ccatgaaaag caacagatcc tgttcgggca ccgcgacggt cacttccagg tgggccaggt
     4381 cctgaaggtt cacgatgggt tggtctcgtt ggacgctctg gaagttttcc acgtgacggt
     4441 ccgcgatgaa accgtcgaag ggagcccgca actccgtgtc ctccagggag cttcgggctt
     4501 ggctcacctt ggcggcgagt cccgcgatgg tggcctccat ggctttgacg tcttcggggc
     4561 gggccccggt gcgggccttg gagagctctt gccctgcggc gttgtactgg gctttggcga
     4621 cctcgtagtt ggccttgtat cgatcgaatt ccgcatcggc cacggcgccc tgctggtgga
     4681 gcttttcgaa gcggcggtat tgggcctcgg cctcggcgag gtgggccttg gcggaggaca
     4741 cggaggccgc tgaggccgcc acgtcttcct tgcgggcccc ggacttcatg gcggcgagct
     4801 gagcctgggc gttggccagg gcactttcgg cgttggccag ttgggacacg tagtcccggg
     4861 agtcaatgcg ggccaagagc tccccccgat gtaccgcctg ggctcgtttt acggggagct
     4921 ccaccagggg gccggagacc cgaaacgcca gctccacccg gtgggacgac tgcaccttgc
     4981 cggggtagga gcgctccagg gcgttcaccg gcggcatcag ctccatggtc ttcaccgggc
     5041 gcacggggcg cagggattcg gcgggcttaa gaggggccgg atcctttccc cggtagaaga
     5101 gaaaacctcc ccccagcgcc actaacgccg cgacgcccca ccaccaggag cgacggtttc
     5161 tttgccgttc ggccatgcgt tggtcacctc ctgataaaga tatgtccctc gaagccactt
     5221 cacctttcga ggtgagatgt agtatagtcc caagggaggg atggggtgaa gagcacgggg
     5281 tggcattttc tcctctttcg atatgttgcg tacggaatct ggaaagatcg gaggattggt
     5341 ccggcatggg gcagggcagg ggcgcggtgt tttagcccga agaaaggagg acggggcgtg
     5401 gaactattga ggtttctttt tcgtgaagcg ctgctctcca tggattaccc ggcgagcgcc
     5461 gatatctttt cccgggccaa ggacgccgtg gggagcccgg aggtggccgc cgaggagctc
     5521 ctgttgccgg tgcttgaaaa gttcggcgag gagtgggagg cggggcagct ttccctggcc
     5581 caggtctacg tggcggggcg catctgcgac gacctcaccg cgcccctggc gggtcctcag
     5641 gaagctgcgg tagaccttcc ggtggccatg gcggggcttc gagatcacca cgccctgggc
     5701 cggcgcatcg tgttggccca cctgcgcatc gggggcatcg ccgtgcggga ttacggatcg
     5761 ggagtcaccc ccgaagaggt ggtggaccgg gcggcgcagg accagatcca ggtgctactc
     5821 ctatcggccc tcatgctggc ttcggccctg gacgtgcgcc gcgtgcggca gctttgccag
     5881 gaacggaact tttctccggc cctcgtggtg gggggcgccc ccttccgcct tgatcccggg
     5941 ctggggcgtg ccgtgggggc ggatgcggtg ggctacaccg cgtcggacgc ccggaagcac
     6001 gtgcgccact ttctcaaaga gagggggatc gggtcatggg gagtgtcatg acgtccatgg
     6061 agcgagtggg ggcggccctt tctcaccgaa aacccgatcg ggtgcccctg gttctgctct
     6121 tcggctatgc ggcggcccgg gaggcggggg tgtcggtcca ggagtacatg gcccgggaag
     6181 acctccaggt ggagactcaa aaggcggttc gggggcgcta tgccctggat tgcctcagcg
     6241 ccttcaccta cttaggggcc gaagtggagg ccatggggag tgaaatcatc ttctcccccg
     6301 aggccccccc ccaggtgggt aagccgatct tttcgcctcc ggaggccctt cgcgccttcg
     6361 aaccgccccg ccccgaagat gcgccggtgc tggcccgtac cctgcgggtc ctcgtcgcgc
     6421 tgcggcggga ctcgggtgat gaggccccca tcataggggt ggtggcatcc cctttttcga
     6481 tccccttgat gcagctgggc tttgagggct acctgaagct cctggcggcc gaccgccagg
     6541 gatctcaggc ggcgggggag cttttggatc tcctatggaa aaaaaattcc cacttcgccg
     6601 agatctgggg ggccgcccaa ctccgggccg gagcccacgc cctgtgctat ttcgaccccc
     6661 tttcttcccc ttccatggtg acccgggaga tgtgggtccg gggggggcgt ccggcggcgg
     6721 agcgattcct cgcggccttt cccggggtgc ccgtgatatg ccacttggcg tccgggcggg
     6781 gggagggcgt gataaaagac ctggccgacc tggggttcgt cgccgtgggc gtgagtgccg
     6841 aagaagacct gggggagcta aaagccgttg cagatcgccg tatcgccttg gtgggcaatc
     6901 tcaatggcat cgccatgcgg cactggagtg ccgaggaaac cgaggcccag gtccgcctcg
     6961 ccgtggccaa gggggctccg gggggaggat ttatcctgtc ggataaccat ggagaagtac
     7021 cctggcaggt gcctccggag gtgatccggg ggctggggga cgccctggcc cgccacggga
     7081 cctactcccc cgactgagga gcggcatcta tgacgatgat cctgagggtc gtggtctgtc
     7141 gggcccttct tcgcgaagtg gaggccctgg tggagggcga ggcggtatgg gtggagggtg
     7201 tgccggaggc gtgttccccc ggggattccc ccccggaaat ggccccggga gaccgggggc
     7261 atcccacgat cttcctgggc tgcgaggccc accgcttccc caaaaatgac ccttggaacc
     7321 gccgtgtggg ggggagccat tgtgcctccc ttctgggggg cgcgtggctc acggcccata
     7381 tcgccgcctc ccggggttac gccgtgactc cttcctgggt ggaggattgg ccccggcgcc
     7441 tggagcgatg gggcttcgac tggcccttgg gacgggagtt cttccacggt ttcgccgatc
     7501 ggctggtgct tttggattct cgcaccttcc cggaggcccc ggagaggctc cgggagtgtg
     7561 cggcggccct ggggctcggc cacgagatcc ttcctgtggg gctggaacct ctgagactgg
     7621 ccttccggga ggcccggagt tccctggaat tggaaggggc ggaaggacgg gttcgagagc
     7681 tccgaaagga agcgggagag gccttcatgg ccatggacct catggggcag gtggcggagc
     7741 tgggaaaaga agatcaggtg gcttcccgtc tccacgacct ctgctccctg ctctttgatc
     7801 cccggagtgt ggcctacgcc ccccggggga agggagctcc gaagaaggtg tggtacttcc
     7861 cgtcctcggc ggaagaaggg gaagagcccc ctgcggaggg ggtggctccg gggggcgtgg
     7921 tgcgagaaga agggggcttt tccttgggag tggcctgggg aggagagacc ttgggagtgt
     7981 tacgggtgcg gggagtggcc gtccccaagc acttggatcg atacgccgac ctcacttccc
     8041 gggtgatctc ccgggtggcg gggcttgcgg tggccaacgc ccggtccttc gagaccctgg
     8101 aaaagaccct tcaggaccga gagtttctga taaaagaaat ccaccatagg gtgaaaaata
     8161 acctgaacct ggtggtgagc ctcctctccc tccaggaccc gggggaacag gacggggcgc
     8221 tgcacaaggc cctgaacgag gctatctcca gggtgcacgc catagcccag atccacgact
     8281 tcctctaccg aggggaggac ctgaggtccg tccctctggg gccctatctg aaggccctgg
     8341 gggcacacct gagggagttc ggcgcggagg ccaataattc cgtgaccctc gtcatttcgg
     8401 gcgatgaagg cctttccctg gaacccaagc gggcggtgtc gtgcggcctc atggtgacgg
     8461 agctggtgac caacgctctg aagtatgcct ttccccccgg ggggccccac ccgcgatctc
     8521 ctgaggtgcg cgtgacggcg gagcgccggg aggggggtat ggtggccctg gaggtgcgag
     8581 acaacggcgt agcgaaggac caagcccccg agacccccaa gggctcgtcc ctggggaccc
     8641 ttttggtgaa cgccctgact caacaactgg agggcaccgt tgtccggtcc catacggaaa
     8701 aaggctacac ggtcaccctt cttttcccgg aaaaagagga ggcctgaagc cgccacccta
     8761 ggtaagtggg gcgccgccgg gcgccccttt cgctttgtgt cggcgggacc ctagggggtt
     8821 ctcctctgtc ccgcttcttc gtaggtcacc ccgtccctaa tgtgatagat cctcttgaac
     8881 gtggggatga tcttttcgtc gtgggtcacg accacgatgg cggtgtcgaa ttgccgggcc
     8941 atatcgttca agatccgaat caccgccaga gctcggtcgc tgtcgagggg ggcggtgggt
     9001 tcgtcggcta agatgatggg ggggcgattc accaatcccc gggcgatggc cacccgttgc
     9061 tgctctcccc cggagagctg agagggcatg gccttggcgc ggtgccccac gtccagggcc
     9121 cggaggagct ctgtggctcg atggcgtgcc tcggcgttag gtacccccgc caacatgggc
     9181 agcagggcga cgttgtccgt gacgtcgaga aaggggatca ggtagggagc ctggaacacg
     9241 aagccgatcc ggtcccgcct caaggcgcgc aggtctttaa ccttccactc tccgtcgtag
     9301 atgacgtctt cccccagggc catctttccc gccgtgggct cgatcaccgc ccccaggcac
     9361 ttgaggaggg tgcttttgcc ggagcccgaa gggccaatga ggcccaccac ctccccggga
     9421 gccacggtca tgtcgacccc tttcagggcg tcgacggcgg cgtccccgct cccatagcgt
     9481 ttttttaatc cctgaatgcg gagtcctcgg ggcgtcatgt cagcctccaa tggcctcggc
     9541 ggggtccacc aggagggcga tgcggatggc gacggtgctc gaaagcaggc atatggccat
     9601 gacgatggcg agacagaggg cggagtcgag ggggagaaga aggacgtact tgggaaaaat
     9661 cggcgccatc aacagagtgg cggagatttt gcccaccacg aaaccgatca gccccagggc
     9721 gagggcctgc ttcatgatga gccccacgat ggtgtggttt ttggtgccga tcagctttag
     9781 tacggcgatc tcccggattt tgcccatggt cagggtgtag atgatgaagg ccacgatggc
     9841 ggagctgacc acggagagga tcaccagaaa caggaagatc tggcgggacg aggtggcaat
     9901 cagcttgccg atcaagatgt cttccatttg gtagcgagta tagaccgtca ggcgcttcca
     9961 gcggcggatc gcggccgcga cctcttccgg gtcatgtccc gccttgaccc gcaccaggac
    10021 ggcgttgacg tagggattgg cgccctggga tgccgagatc gcttccagca ggccaggcac
    10081 tccggggcgc tgaaaggcgg ggttttcggc cgtgcgccgc cgttgctccc ggatggcgtc
    10141 gttgtctttc aaaaactgtg cctcctgggc gtctttcagg ggaatgaaga ccatggggtc
    10201 gccgccggaa gacaccatcc ggcgggttaa acccaccacg gtataggtgt ggcggcggat
    10261 ctggatggac tctcccaagg taaggcccga agcgacgtcg gcaacggcct cgtagtggcc
    10321 ccgggtgatc gggcgtcccg ccacgaggtg gggaggccat ccgggggttc ccggcccccc
    10381 gggagcgatg ccggtgacca tggcccgcac ggccccgtct cgggttccca ccggcatggt
    10441 gaggtaggtg atattggcgg cttcttccac cccgggcatg cctcgaagac cccgccagac
    10501 gtcgtcgtag atgctcgacg gttcggcata gggccccagg gtgtcttttt gcaccaccca
    10561 gaggtcggcc ccactgccat cgagcaacac cttggcgtca tccaccatgc cgcgatagat
    10621 ccctgccatg gacagggtga tgccgatcaa taaccccagt cccaggccgg tgaagacgaa
    10681 cttcccccag gagtgcatga tgtcgcgtcc cgccagactg atcaccgcgt cacccccttg
    10741 atatgatcca cgatgcggat ggatcggtgg ggggtcaggg cgctttcgct gtagaccacg
    10801 acctggtctc cttcctcgag cccttctcgc acctgcatta atccttccag gtcggcgatg
    10861 cccggagaga cgggcacaaa ttggagatcg tcgctaacca ctcgccacac tccccagcgc
    10921 ccctcgacgc gatggagggc cgcctgggga atgaccggtg ccgccgccac cgcaggcagg
    10981 gcgaccgtga cttcgactag ttcgcccacc gggggcctgg ggtcggggag gcggtcgaag
    11041 acgaccttgg ccatggtttc ttccgtgacc ggatcggcca ggggatcgac ccggagcacg
    11101 tgccccgtct gggggttttc ggctcgggat cggagggtga tctgggcagg gagccccgcg
    11161 tcgaggcccc cggagcggat ctgatcgaat cgcacatcga cccacagcgt gtctgggtcg
    11221 atgagctcca ccacggcctg ccccgccacc acggtggtcc cgggatcggc gtcgcgccgc
    11281 accaccaggc cgttgacggg ggccaccagg gccaagtggt tccgctgggc ctccagggct
    11341 tctcgctcgg cgcgggcccg ggcgagctcc tcccgggcgg cggccagtcg ggcctgggcc
    11401 atggacagat cttgtcgttt ggcggacgcc acttcctcgc tggtggagtg gtccttgagc
    11461 agcttgtcgt agcgcgacgc ctgggtctgg gcgtggtctt tgtaggtctg ggcctcgtta
    11521 accttggcct gggcctgctt tatggcggcg tcctgggcct tgaggcgttc gtcgagatcc
    11581 accgcctcca tctctcccag tcgttgcccc gcctggaccc ggtcgcccac ctggacatcc
    11641 aggcttttga cccgccccgc cagggtggga cccatcttgt agatgtggcg ggcctcgacg
    11701 gtgccgatac caaagatggc gggcgacagg gccttgatct tgaccggggc caacaccacc
    11761 gctactgggg ctaaggggcc cgagcgcagg gccacgtaga caaaaagggc catcaggggg
    11821 gagaggacga cggcgagggc caggaggcgc tttccaaagg tcattttttt catgccatcc
    11881 tcctaaggcc ccgtcggtat agggcaaaaa ttagcggcgc gtcgtgacgc acccggctcc
    11941 catcgcccgc gatcatcgat tgcaccacca gtccctgaag ggcgccgata aagagcgtgg
    12001 ccgccgcttc gacgtcgagc ccttcgtcga gctccccgca gtctcttccc cggtggagaa
    12061 gggttcgaag cgattccttg tagcgggcca tgatggcctg ggccatccgc ttggggatgc
    12121 gatcttcggg gtgttgtagc tcgccgaaga gcatgcgggg aatgccggga tgggacgcga
    12181 taaattccgc atggaacaga aatagcccct ccagggaggc cagaggggag caggccgccg
    12241 aggtcgcctc ttcggatcgg gagatcaggc gttgggctac ccagtccatg gccgcttgta
    12301 aaatggcgtc tttgctgggg aaatggcgaa agatcgcccc ttgggtcagc cccatgcgct
    12361 gggctatggc ggcggtggtg atctcgctgg ggttttgctc ccccgccagg gagatcatcg
    12421 tttccaccgt cagggcccgc cgcttttccg caggaaggtt ttttttcgtt tggttcatgg
    12481 gctcctccaa aaaagatagt tattggtcac tatcttattt tcccagggat ctcccgtcaa
    12541 ccggtaaaag ggcggattct cgccgccgaa ggcccggtcc cgtcggaggc aagaccccgg
    12601 ggcggggagc gcgcttttcg ggcttttgtc gcccatcgtg gggcccctcc tcctgtttgt
    12661 ccccgtaatg ccgccctttc ccggcggagg tccggggcgc ttcgacatcg cacctggcgc
    12721 tcatggcggg ccggggggtt gacgtcgacg tcctgaggtg gtatggtgcg aacatggata
    12781 ctacgacgag ctcttactgt ttcgtccaag aaggcctttg gtggtggcgc ccctagcgcc
    12841 accgccgttc ccgtttgtct tttcggggct gcggtgcgga tgacgcatac gcagcccctt
    12901 ttctttattc accgagcgat gaggggcttc cgtggggagc ccctttagct ttttttaaat
    12961 accccagagg aggaagagga gcatgacgag cacagccaag gcgagccaga agggtttttt
    13021 cggagagtac ggcggcagct ttgtgccccc cgggctacag ccccggttgg atgaattgga
    13081 acgggcctac gaggaggccc tgagggatcc ggcctttgcc gaagagctca cggagctact
    13141 gcgagactac gtggggcgcc cctcggccct gactttctgt ggcaacattt cccggaagta
    13201 cggtgggggg cgtctcttcc tcaagcgaga ggacctgaac cacaccgggg cccataagat
    13261 aaataacgcc ctggggcagg ccctattggc gaaacgcatg gggcggaagg agctcattgc
    13321 cgagacgggg gcggggatgc acggcaccgc cagcgccacc gtggcggccc tcatgggaat
    13381 gaagtgtacg gtgtacatgg gggcggtgga cgtggatcgc caggccccca acgtggcccg
    13441 catgaaggcc ctgggggcca aggtggtggc ggtgcaggag gggcaaaaga ccctgaaaga
    13501 ggcggtggac gtcgccctcc gagcctatgt ggaacgcccg gagagctttt atcttctggg
    13561 gtccgccgtg ggaccccacc cctatcccgt catggtgcga tctttccaaa gcgtcatcgg
    13621 ccgggaagcc cggcggcaat tcctggagcg cgaggggcgt ctgcccgact acgccgtggc
    13681 ctgcgtgggg ggaggaagca acgccatcgg cctctttgcg gcctttctag acgatgccgc
    13741 cgtgcgaatc gtgggggtgg agcccgctgg acgggggctc acccctgggg atcatgccgc
    13801 caccctaacc atggggtctc cgggggtgat ccacggcttc cggagctacg tgctcaccga
    13861 cggccaaggt gagcccgccc cggtgtactc catctccgca gggctcgact atcccggggt
    13921 ggggcccgag catgccttcc tcaaggactc ccgccggggg gagtatgtca cggtgacgga
    13981 ccaggaggcg gtgggagcct tccagaccct gtgccacctc gaagggatca tccccgccct
    14041 ggagagctcc cacgccctgg cctatgccct gaagctcctc ccgaccctat ccccggaaaa
    14101 gacggtgctg gtgaatctct cggggcgggg agacaaggac atggccacga tcctccccct
    14161 gctggacctg gcggactgac ttgccctttt gggggcgctt cccctaggcg aggttctttt
    14221 agccctgggc gccccctttt tatcgaaatc tttcttggcc cgttttagag ttcttttccc
    14281 tccctttacc caagcccatc acccccagag tcttacttct tcgacccctt ccccatagcc
    14341 ctctgcggca cgcgccgaag ggggcatcgt caccgcctaa gattatcgca gagaaaaatt
    14401 tgccccggga ggggaaaaag ggccctagcg gagtgctttg gcacgacaaa tgcgctacta
    14461 taatgcccgg acttccagcg gcggccccag agcggggatg aaggagcgtg ggacaccgtg
    14521 ggtttttacg tggatgtggt gggatggcgg cgcatttcct ccgaaatacc tttgtcgggg
    14581 cccgatgtgt tgcgagagct gggcatcgag gggcgccgcg aggtggtggc ctggcgggtg
    14641 aaccgttacc tgaggtcttt ggattggatc atcgacgagg acgcgacggt ggagctggtg
    14701 gacgcctcct ccttcgaggg tatggaggtc tatcgtcgta ccctgagttt tctcctggtg
    14761 ttggcctgcc gtcaggccct gggatgccag atctccattc ggcactccat cagtaatgcc
    14821 tactactgtg agctttcctg cggccccgcc agcgccgacc aggtggctcg catccgccag
    14881 gccctgcggg atctggtgga tcaaaacatc cccatccgcc gatccgtggt gtctttggac
    14941 aaggcccgga ggatcttcga ggcccaggga aacgacgata aggcccggct tcttcaatgg
    15001 gccgtgcagg accccgtgga gctctattcc tgccaggggc ggtttggctt ttactatgcc
    15061 cccttggccc cttcctcggg ggccgtgccg ctttttgacc tcatctccta tgctccgggc
    15121 atggtgctcc gctttcccac catccactcg cccttttccc tgccggattt tcaagccact
    15181 cccaggctgg tggacgtctt tcgggagtac ggagaatggc tggacatcct ggacgtgggc
    15241 accatggcga gcctccacga gcaggtggcg gaggataaga ccgtggatat catcatggtt
    15301 tccgaagcct ttcacgcccg gcgtttggcc ctggtctccg aggagatcgt ccggcgtaag
    15361 tccgtgcgcc tggtgtgtat ggcgggaccc tcggcgtcgg gaaagaccac ggcctcccga
    15421 aggcttcggg tgcagttgca actttcgggg ctccaccccg tggccctttc gctggacgaa
    15481 tacttcgtgg accgggagcg caccccccgg gacgaagagg ggcagtacga cttcgaagac
    15541 atcaatgccc tggacctgga gctccttaac tcccatctcc agaggcttct ggcgggggac
    15601 gaggtccagc ttccgcgctt cgactttctt ttgggcaaaa ggaccccggg gaggaagctc
    15661 cgtttgaggc ccaaagacat cctcatcatc gagggcattc atggcctgaa cgatcgcctc
    15721 acggaggccg tgccggagga cctcaagtac ggcatcttcg tgtcgcccct cacgggggtg
    15781 agccttgatc gccacacccg gacgagcacc acggacaacc gcttcttgcg acgtatggtg
    15841 cgagatcacc gtacccgggg caagtccccc gaaaacacca ttttgcagtg gccctccgtg
    15901 acccgagggg ccttcaaaca cattttcccc tttcaagatc gggcggacgc cctgtttaac
    15961 accgtgttgg tctacgagct ctccgtcctt cgggggtacg ccgagccctt gcttcgcacg
    16021 gtgcccgaaa gctctctggc cttcggcgag gcccagaggc ttttgtccat gctccgcttc
    16081 gtgcctgtga tcccggcgga caacgtgccc aatacgtcca tcttgcggga gttcatcggg
    16141 gggagctgct tcggcgacta aggacccgga ggccttcgcg gcgaaggatt cttcggcgcg
    16201 gggatttttg ccgatttttc tagttcgcgg caccacgccc gccccttcac cggtgaggcg
    16261 ggccaaggag gagagagcat ggcggaacgc atcgcggtga tcggcacggg ctacgtgggg
    16321 ctcgtggcgg cggtggggtt ggcggatttt ggcaacagcg tggtgggggt ggatgtggac
    16381 cccgaaaaag tggctaagct ccaggggggg gtttccccca tctacgagca ggccatggag
    16441 gaatatctgg agcgaaacct ccgcaacggc cgtctgtcct tttccaccga tgtggaggcg
    16501 gcggtgcggt cgtcccgcct ggtgttcatc gccgtgggca ccccggaggg tcccgggggg
    16561 gatcctgacc tatcccaggt ggaggccgta gcccggctca tcggccgatg ctgcgcggac
    16621 tacaaggtgg tggtgatcaa atccaccgtg cctgtgggaa ccaatcggag gatcgcccgt
    16681 ctcctccggg acctggcggc ccccgtggag gtggacgtgg tctccaaccc cgaattcctc
    16741 cgagagggga gggcggtgta tgactttttc aaccccgaaa gggtggtcat cggcggggag
    16801 tctccccggg ccaccgccat cctccgctcg gtgtaccggt cacgccacct ggacgaaacg
    16861 ccctttctct tctgtagctg ggagaccgcc gagctcatca aatacgccag caacggcttt
    16921 ctggccacaa aaattacctt catgaaccag atgtcccagt tggcggagtg tgtgggggcc
    16981 gacgtggcca ccctggccaa gggcctcggc atggacagcc gcatcggccc cctcttcctc
    17041 caggcggggc cggggtacgg ggggtcgtgc tttcccaaag acaccatcgc cctggtccgg
    17101 acgggggagc ggtgcggggc ggacatgacc ctgatccgcg aggtcatccg ggccaacgag
    17161 gcccacaagg cccgcatggc ggaaaaggtg gagcggctcg tgggccctct ggtggggcgc
    17221 accgtggccc ttctcggatt ggccttcaag gcgggcaccg acgacgtccg agagtccccc
    17281 gcgctggtca tcgccgagga gctgctgcgc cggggggccc gggtgcgggt ccacgacccc
    17341 aaggccctgg agaacgcccg gaagatcctg ggagaccggg tggcctactg cgccagcgag
    17401 ttcgaggccc tcaccggggc ccacgcagtg gtcctcgcca cggagtggaa cgagtaccgc
    17461 aacctggacc tggagcgggc ccgggggctc atggccgccc ttgccctggt ggatctgcgc
    17521 aacctggtgg aaccctcgga ggcccgggct cggggctttg cctacgacgg cgtggggtac
    17581 ccggggccat aggtccctca ccgcaagagg taggtgcgcg ggttcgatat tgccggggcg
    17641 gaaaatggat catccccgcc gtttccacgg atgattgcat gtttaacttg taggggcgaa
    17701 aaatctgttt atcctcaccg ttccggcgga tgatcacggg tttaattcgt aggggcgaaa
    17761 aacctttcgc tccaccattc cgcggacggg cacgagctag atttgtaggg gcgagcgtat
    17821 ttttcgcccc catacttcct taaaactccc cggccctccg gggtgacctc cacggcatgc
    17881 tcaccgttcc cgcccgcccc tcaatggggc gttgcaccac ggggtacacg gggagggttc
    17941 gccgatccaa cgtcgctgcc acgtccccca gggtacaaaa gacaaatccc cggtcccggg
    18001 cccgccgcag aagttcctcc agaaggggaa ggtgggcccc cccctccatc tctgcgtgga
    18061 cggtatggat gtggaggttc ccccgggaaa ggtggtccag ataaagatca atcacccctt
    18121 cttccgaaag ccccggagcg ccgtagatct cgtccagcgt ggggagcgtg gtgggcatct
    18181 ggggcgtgcg gagtacggcg gtccccacct ggggcagaaa gggctccccc gtgcggacgt
    18241 cgctgcagta agcaaaccgg taccgctcct gcatcatgag actctcggcg ctggtctgcc
    18301 accccggggc ggcacagctt tgggcttcgc gccccgccac ccgggcaaaa agctccagag
    18361 ctcggtcaaa atcctcccga agccggacag gggaagtgga aagcagggtg tcctgccacg
    18421 ccacatggtc ccaggcgtgc actccgcagt cgtgccccga cgtcaccgcg tcccggaggg
    18481 gctccggcgc ggagggaaca atgagtggag cggggagaag ggtccccgaa agcaccgtcc
    18541 gccatccgta ggtggacacc gctttggtgc gccgcatctt cgccagaaat cccggacgga
    18601 agatccgccg cagggccttg ccggagttgt ccggccccag ggagaagaaa aacgacgccc
    18661 gaagctccag ccggtcgaag agccgcaaca gggcgggcac cccctcccgg tgtcccgaga
    18721 gggtgtccac gtcgatcttt acgcagaggg tgggcatggg gtcgtttcca ggatgctcgc
    18781 cacggtgcgg cgcaccgttt cggtaaagcc gatccggggc acccacccca aaagttcccg
    18841 ggccttggcc accgaaggcc gccggtcgtc cacgtcctcg tatcccgggc cgtagtatgc
    18901 ggcggcgggg cgatcctcca gcacggcggc ctcggcgacg gcccgccagg cggggtgctc
    18961 tttcagggcg tcccgcaccg ccagggccag ctcccggatg gaggcgttgt tgtccggatt
    19021 tcccaggtta aaaatctgcc cctgagctcg ctcctcctgg gtcaggatgg ccaaaagacc
    19081 atcgatgccg tcgtccacgt gggtgaagct ccggcgctgg gctccgccat tgaccagcac
    19141 cacggggcgc ccgtggatca tgtcgtagag catctgggtc acggcccggg cctcgtggcg
    19201 ggccgcgtcg tcgaaggtgt ccaggcgggg ccccatccag ttgaaggggc ggaagcagga
    19261 gtaccgcagc ccccgctcct ggcccaaggc ggcgagcacc cggtccatca tctgcttgga
    19321 gcagctgtag atccatcggg aagcggcgat gggccccagc accagggggc tctcgtcctc
    19381 cttcagctcc cggtgggggc tcatgccgta gacctcggag gtggagggaa agatgatccg
    19441 ggtgccgtgc tcggcgcaca gccgggcgat tttcacgttt tgctcatagt ccagctcgta
    19501 gacgtacagg gggcgtttca cgtagagagc gggttttgcc acccccgcca ggggaaacac
    19561 cacgtccgat gccgccaccg cctccgcgag ccaggcatct tcccgaaaaa tgtccccctg
    19621 gcggaaggta aatcgcgggt ggtgggccca ccgggccaca taatcccccg ccagatcgaa
    19681 ccccgtcacc tcccactggg gatcagccaa gagccgctcg ctcaggtgac ttccgatgaa
    19741 gccgttgacg ccgagaataa gaattttcac ggtatacctc ctgaaaaatg acgtcccata
    19801 gcctaaaacg tcggcgaggg aacggacggt gtgccaaggg gctttctgcg ccaaagagac
    19861 tccggtcagc ccccgtcttt tttcgcgata tttctcagca catggagaaa ccttgccccc
    19921 cagggactcg ccgacgaaac tttctccggg tcaatctgca ggaggagggg gaaagagtgc
    19981 tcccccttgt cgtagggggc ctttgtttta cagtgggcgc tggcctttcg caggctttca
    20041 tagacttctt tttttgagag acgttccaca tttccccgct gcggtaaggc cccggcgttg
    20101 aacccggggc caaaaaatcg agagagggtc tcgtgatcgg ccagcaacca tgactccata
    20161 cagaccacca tgaggtgaca gtggtcgtcg ttggtgtggc aggggggaaa aaaaccttcc
    20221 cgtcggaggt gctcccaagg agagtgtccg gaaacttttt cctcgctgtc gatcaacaac
    20281 agagccgttt cctggtgcgc gtcgcaggcc acgcggaatc tttcgtaggc ggcttggcgc
    20341 ccgccgcacg ccacaatgcg gggcatttgt cccttcagcc ctgcccgctc caaaaacaca
    20401 cggaaccctt ctcggcagcg ccggtggagt ctttccgaat ctcctccgcc ctcgacgtac
    20461 agcttgacta ccatcggttt cctccgatct cgccgctggt ccagaggtgc cccaggccgt
    20521 actgttggag ccacgggcgt agcgccccct cgtcaaggcg gtggacggag gtgtgaaagt
    20581 tttctttttc gcagaccagg acgacctccg gcgtgttcgt gaaggcgtct accagaccgg
    20641 tggaatgggt tgtcaccaca atctgcgttc tctcggcggc caggcgtagg gcgtctgcga
    20701 cgtgattgat caggtccggg tgtagcccaa tttccggttc atcaatgcag acaagagggg
    20761 gtggggtggg gtcgcagaga accgccagaa gacaaagata acgcaacgtg ccgtcggaaa
    20821 gccttgtggc gggaacagaa aatcgtccct cctgcacgaa gacctgcacg gtgccccctt
    20881 cgaccatgac gtcgaaatcc tccgcctcgg cgtagacggc gcggaacatc tccagcagcc
    20941 gactcttggc gccgctgtcc cgccgtaggc ggttgagcac cagccccaga ttacgcccat
    21001 tctcctcaag ccacccattg ggcagatcgg cgggttgggg gagacggggc ggcgtgtaac
    21061 gccccaaaga ccagtcgcgg tagatgcgca tgcgtccgaa gacttctccc agacgcgtga
    21121 tttccggata gatatcgggg tcctgacgct gggcgagaat ggaccgtgac gggtcgacat
    21181 cctcccgacg cagcgtccgc caccagcgtt tctcgtcttc cgggtcgcgc acgctgagca
    21241 caggatgccc ttcttcgtac cggtagtaga aaaaaggccc gtcgtgtccg gaacacggcg
    21301 cggtgctctc gatgcgttca tcggtgatct cgaaccgccc ccccgatgcg gtgaaatcca
    21361 agcggtaccg caggggaagt ttcccctcgg tgccggggaa aagttccaca agggcctcta
    21421 tggtggctgt ggggatttct ttcgtcccct tccacaacca gtcccgcacg ccgccccctt
    21481 ctcggatcgg cgtgtccagc cgcgaaggag ccgattgcag cagggcgatg ccctcaatca
    21541 gattggattt tcccgagccg ttaggtccga tgagaacgtt caatgctccc aggggcacgg
    21601 tctctccctc ggggccgtac gaaagaaaat ttttcagccg gaggcttgtg atcatcgtat
    21661 ccacctctca tgccggcatt cccctttcgg agcactcgcg tctccgtcct ttccggtttc
    21721 agtccggctc tttttcagaa cttttacgca tctagccgct ctccggtgtg cagatgtgtc
    21781 gccccccaga cgttgccgtc ccgctcttcg tcgccctcct cctgcacccg gtccagacgc
    21841 agcacgcccc ggccggtgcc cacccgcagg ggagccaccg agagcacttc tcccggcgat
    21901 atttcttccc cctcttcggg aaaagcccgc cagacaagga tcttcttttc cccaagagga
    21961 aagaacgctc cgggccaggg gtgggtcacc gcccgcacca gatcgtagag ctcccgggcg
    22021 gagagctccc agtgcatccg accatcttca gggccccggc ggccgaagga ggaggcccgg
    22081 gaagcatcct ggggaaagcg aggggccgtg cccccttcca ccagaggcca cgtgcgggcg
    22141 aggatccggg cggcggcgtc ggccaccttg agggacaccg acagagcggt gtcctcaaag
    22201 gtgatggaca ccccctcctg atccagcacg tcccccgcgt cgggacgggc caccatctcg
    22261 tggagcgtgg cccccgtctc cgtctctccc cgcagaatgg cccagttgat gcacgcccga
    22321 ccccggtagc gggggagaag ggagccatgg acgttgaacg ctccgccggg gggcagggcc
    22381 agcacctccg gagcgatcat gtcccgatag tacgccgaaa gaagtagatc gggggccaac
    22441 ccttgcagca actcccgccc ttccggggag cgcaggcttt caaagagcac cggaatgccc
    22501 gccgcacggg ccccgtcggc caccgatcgg aaccagatct cctcgtgggg atcgtcccgg
    22561 taggtgaaga ccgccaccac agaagccccc agggcgcaga gggtctccag acaccgcacc
    22621 cccacctcgc tgtaggccaa aagcaccacc cggggtcgtc gccccgccgg aggaaagctc
    22681 cgcacctcag ccacggcgat ctcctcccgg agagagggga tccttcgcgt cgtcgtagat
    22741 ctttcggatc agaaaacggg ggcgtcgccg cacctccaga tagattctcc ccacgtattc
    22801 ccccgcgatg ccgatggaaa gcagcgtgat tcccatgaga aaaaagttca gagccaggag
    22861 cgtgaaaacc ccctccacct cgggacccaa caccagacgc ctcaggagca gaaagaccgc
    22921 gaagaccgag ctcaagaagg aaaccagcgt ccccagcatg gtcaccgcct ggaggggcac
    22981 cagagagaag cccgtcatga ggtcaaaatt cagccggatc aggcggtaca ggccgtactt
    23041 cgacgccccc tgctctcgct ccgcgtgccc cacggcgatc tccaccggat gggtggcgaa
    23101 ctttcgggcc agggccggga gaaaggtggt ggtctcctga cactgattga tgtaatccac
    23161 cacccggcgg tcgtatcccc ggagcatgca gccgtagtcc tttagtccca ggccggtgat
    23221 gcggtttgta aacttattga cgatgcggga cgccaccttg cggaagaggg gatcgtgccg
    23281 attttcccgg tatgtgccca ccacgtcgtg ccccgcctcc agctccgcca gaattcgggg
    23341 gatctcctcc ggcggattct gcagatcggc gtccatggtg atgaggtacc gcccccggga
    23401 gttttcaaag gccgccagaa tggccatgtg ctgaccgaaa tttccgttaa aatccagcac
    23461 ccggacgcag tgggggaacc gatcccggtg tgccagaaga agccccaggg atccgtcccg
    23521 gctaccgtcg ttgacgaaaa tgacctcata gggcttttgc agcgcctcca gtacgggcat
    23581 cagccggggg aaaagccggg gcagggattc ctcctcgtta taaacgggaa tgaccaccga
    23641 cagcagcggt gccgatgcgg ggtcccgagg ttccgtggac acaagaaacc gccttcctct
    23701 cgtggatcat ggatgtcttc atagacatga aaaataacac caccgtaggg gcgagcgtat
    23761 ttttcgcccc tacaacccct acacgcgaaa ctcgcgccta tcggtgtatc ggctgtgggg
    23821 caattgcatt tttcgctccg acaattggca ggagccccag ctagggatgc tcaccttcca
    23881 actggccggg agcgcacgcc ttccagccgg ctcttttttc agccatcggt cgccccacca
    23941 acgcaaccct ttcgcgaaaa ccactggacg caagccggga gcgccgactt ctagtcggct
    24001 ctttttgaac tcccggatgc tccctaccga aacctgcctc aaaatgttaa cgaaatatca
    24061 ccgtaggggc gagcgtattt ttcgccccta caaacggaat ttgtgcccat ccgccccgac
    24121 aacgcctacc gatttctcac caccacttgc ccctgatcct cccccagaaa acgcccgggg
    24181 ggaaagcccc cccgcaccag atcctcatac gcctcccggg gggccaccag gatcatcccc
    24241 ccgttccctt cccaccgggg acggaagtcc gccgaagaaa taaaccactc cccggcccgg
    24301 tcctgagaag ctccaaaggc cagctccccg taataattca ccaaaagaac ccgctgcttc
    24361 aggtaaaagg gtagcccctg gtgatattct cccacgttgg ccaccccgtc ccccggctgc
    24421 cggtgcgccg ccaccgtcga cgccagccct cgggcggaga tgatctcccc gtaaaagctg
    24481 aacaccggct tcatcgccgc cccctgcacc agggccaccc ccagaagtat ccctcccaag
    24541 gcccagggac gacggaccag ggcccaccag aggagcagca ccccccccag aagaacccct
    24601 ccccagggca gtcccagggc caggagccgg gaaggatcgt acttgtcctg gaaaaaaggg
    24661 tagaccccca gggccagggc caggggaagc atgaaaagcg tcgaaagccc cactccccag
    24721 aggagggatc gccgtcgccc cagagccgga ggctgggcca gggcccatcc ggtcagcagc
    24781 gccagggggg gcatcgctgg caggatataa ggtaccagct tcgaccgtga tagggagaaa
    24841 aagaccaaga tcacggcgaa ccagagcaac ataaagagca gattttcccc atcggcacgc
    24901 ccctcgccct ctccttttag ggcctttccc agggcggcca gggccatgcc ggtccacggc
    24961 aaaaatccca ccgccagaat gggcaggaaa aaccaccagg gctcgaagcg tccgtggacc
    25021 atggtggtgt agcgcaaaaa gtgctcgtgg acaaaaaaga accagaaaaa ttcggggtta
    25081 gcccggatta ccgccaaaaa ccacggagaa gccagggtga agaagaaaag cactcccgca
    25141 aaggaaaacc cccggcggac gtactcccat cggcgggtta acacgatcca ccaaaagatc
    25201 accgccccgg ggagcaccac cccgatgagc cccttggcca aagtcgccag ggctgccccc
    25261 ccgtagaaga gcgcgagcca cccccgagac cccgtcatgg cccgatggaa ccccagcaga
    25321 gagagggtca ggaaaaacgt cagcgtcatg tctgtgatgt taatgtgggc tgaggcaaac
    25381 cacaaaaggg agctccccag caccgccccc gccgcgatcc ccccccgggg cccccagagc
    25441 gatgccccaa agagcgccac cgccccggca ccccccaaag aggccagggc gggcaccacc
    25501 cgggaggcga attccgtctc ccccaaaagc cgaaaagctc cggcggtgag ccagtagtgc
    25561 agcacaggtt tctcgaagta cttcacatag ttgagccggg gggtggtcca atcccccgtc
    25621 tcgatcattt cccgggggat ttcgccgtag cgcccctcgt cgggctcaag caacccgtgg
    25681 gatcccagct tccagaagta gagcaccccc agggtcagca gcaggatccc caaacggggc
    25741 agaaaggctt tttcgtcagg aaggcgcata agaggggtct ccttcgacct cggtcgcgac
    25801 ctttcccgtg tcccgccagc gccgggccag atagataaag ggcgtgtcac agagggctac
    25861 cagggccttg atgaggtagg tgctcaagaa aatctgcccc agctcctccg gcggaaagac
    25921 cccccagaag gccaccccca cgaagatcag cgtgtccagg gcctgggaca ccaaggtgct
    25981 gccgttgttt cgcagccaga gaaaacgcac cccaggaaaa cggcgccacc agaagtgata
    26041 ggcctccacg tcgtgaaatt gggagaccag ataggccaga aggctcgcca ccgccaggcg
    26101 gggcaggagg gtaaagatcg tttccagggc gggggaggcg aagtctgagg cgtcggggcg
    26161 aaaaagcagg gtaagccgca tgagcaccgt gaagaccacc aggctgaaaa acccgatccc
    26221 cactgcggtg cgggcgtcct ggcgcccatg gttttccgaa aggatgtccg tggcgaggaa
    26281 cgtggtgccg tagatcacgt tgcccaacgt ggccaccagg ccaaagagcg acacggtctt
    26341 caccacctcg atgttggcga tgacggaagc cagggccacc caacaaaaga gccccgtctt
    26401 gccccagagc cgataggcca agaggatccc tccgaaattt gccagtaccg tgacgaaaaa
    26461 aatcgtttcg tttcccatct cccgtgagcc tcccagtccc ggtgtgttct gcccctcgga
    26521 ggccatttta ccagaaagcg caagatccgc ccccccgccg acgggattat tgtaggggcg
    26581 aacgtatttt tcgcccctac aacccatggc catggtggcc gcaccgccgg gagtccccac
    26641 caccgatggg cgcacggccg aagagcacgc accttcccgt cggctttttt agcctgccat
    26701 agccgaagag cgccccccgt cccgccgccc cgactccacc cttcgccccg gg
//
LOCUS       DAOOER010000026        25192 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_2281_length_25192_cov_9.619565, whole genome shotgun sequence.
ACCESSION   DAOOER010000026 DAOOER010000000
VERSION     DAOOER010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 25192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25192
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2281_length_25192_cov_9.619565"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            385..549
                     /locus_tag="PL162_05095"
     CDS             385..549
                     /locus_tag="PL162_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91432.1"
                     /translation="MYSNKLVYIGTTPYWKTPEGRFEPVECREGRMIDRVFLRTPQGL
                     RPMENGCSPV"
     gene            complement(844..1896)
                     /locus_tag="PL162_05100"
     CDS             complement(844..1896)
                     /locus_tag="PL162_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQF91433.1"
                     /translation="MDRDRIFLEELVRCASPSGAEQEIGALLVSRLPALGWERVELDD
                     VGNVVASRGKGPREVLLLGHVDTVPGGPPVRWEGEVLWGRGAVDAKGAFGAFALAGGA
                     CAVPEGGRITLVGAVGEERDSRGARYRLMRHAPEACLVGEPSGSDGVTLGYRGRIFCT
                     LEARDGGAHRSGDAGPHTACLRGAAAVLDSWERGDDPTRPVIERPSGAVAFMEGREEG
                     ERRGRVELDLRLPLGAEPERVVQEVREVAERWGVTVYFSEKVRAHLVPRDDLAVRALR
                     GAIRQVFGRAPRLLAKGGTADFNLAASWGCPLAAYGPGDSRLDHTAEERLPLEEFRAA
                     VAVLQEALPRLWREEA"
     gene            complement(1886..3019)
                     /locus_tag="PL162_05105"
     CDS             complement(1886..3019)
                     /locus_tag="PL162_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQF91434.1"
                     /translation="MPSAGYGSRGLTAVRGAGDRLWDAQGREYVDFLAGHGAALFGHA
                     HPELVSVLRESAASCWTLGAGLSHPARSRFLSLLDGLIPEGCSFLSNSGAEAVEAALK
                     LVIALRPGKKRLLALRRSFHGRTLGALALTFNPKYRKPWQEQLCAVEHHTSEALLEAV
                     EDDVAAVFVEPVQGEGGVHPLDPAFGGALSARCRETGALLVADEIQSGWGRCGAVLAS
                     SGAGLDPDVVCLAKGVAGGFPVGVTVWRKNLGDFPVQGHGSTYGANPLACALGEATWE
                     LLHRHDLFRWAEERGRAFREELSRVDAPGRGEVRGRGLLVGVEIEGESGAVVRELQRR
                     GVLALPAGPRVVRFLPPFGASPESFLRAATALGEALRELSRGS"
     gene            complement(2986..3816)
                     /locus_tag="PL162_05110"
     CDS             complement(2986..3816)
                     /locus_tag="PL162_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[LysW]-aminoadipate kinase"
                     /protein_id="HQF91435.1"
                     /translation="MFGVVKIGGAPGNGAENVLRDLAERVGRGERWVLVHGASGVMDG
                     LCRSAGVEVRHVVSPSGFRSRFVGEGERELFEAAAARVSLEHVQSLGELGVRGVSLWP
                     REGSVTGERKEVLRAVESGRCRLVRGNWSGTVRHVDPAPLRRAWSRGEIPVLPPLAQD
                     GVSGRNLNVDGDRLAAAVAVALGAEALVILSNVPGLLRDPQDPDSLVASGDLEDWGEL
                     EEMARGNMKRKLQACREALEGGVPRVLLGDSRGRRPLEEVLAGRGTELCRARATALAV
                     "
     gene            complement(3839..4885)
                     /gene="argC"
                     /locus_tag="PL162_05115"
     CDS             complement(3839..4885)
                     /gene="argC"
                     /locus_tag="PL162_05115"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HQF91436.1"
                     /translation="MRRVGIWGASGMAGGEVLRLLARHPEMETVRAVSARHAGEPVWH
                     QHPHLRHALGDMVFSSPEEARRRAEEVDLVFLAVPHGAGADIAAFHVARGVRVADMSA
                     DFRLRDPEDHRRWYGTPHPCPDLLARAVYGLPEFHREALRETPVASGVGCNATCTNLG
                     LRPLAARGLIREARIEVRAGSSEGGGTPTVGSHHPYRRGALRVVEPFRHRHAAEVAQE
                     TGMPLSAFSFTLTAAEMVRGAQMLAHVTLTEAMEEKALWRLYREAYGGEPFVELCPAR
                     PAHLRFPDPRRVLGSNRALVGFALDEGGTRLLVGCAIDNLMKGAAGSAVQCANLMLGF
                     EETAGLDALAVYPA"
     gene            complement(4967..5794)
                     /locus_tag="PL162_05120"
     CDS             complement(4967..5794)
                     /locus_tag="PL162_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HQF91437.1"
                     /translation="MVVRGRPTEVGEGYLAFDGTAVERWIAEVSPAEVWFRLIQGRLP
                     GEGERRLWDAVLATLADHGDTPPSTQAARLVASTGVPLQASLAAGLLAFGNHHAGALE
                     GVMEILQSGVARGLDPEALVDEALASGRRVPGFGHRLHRRDPRVAPLRCLAEERSLRR
                     DHLAFFSRMEDLLGERKGIGGNVDGICGAVLSDLGFPFQAGRAFFLAGRLPGLTAHIL
                     RETSGGSFRRYALREVGEESSPLREGLGRAPGGGFRVSGRREAREIRRHREPQGERS"
     gene            complement(5785..5952)
                     /locus_tag="PL162_05125"
     CDS             complement(5785..5952)
                     /locus_tag="PL162_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91438.1"
                     /translation="MGIVRLFAGEASGDLEIDITVRARRNEEVGREECGGRRELPLLP
                     EEHLGRDGEWW"
     gene            complement(6224..7069)
                     /gene="lysX"
                     /locus_tag="PL162_05130"
     CDS             complement(6224..7069)
                     /gene="lysX"
                     /locus_tag="PL162_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysX"
                     /protein_id="HQF91439.1"
                     /translation="MARLRILYSRLRTEEKLLAAAASREGIPATLEDVTDGVFGERLP
                     AAQGDVVLARCISHSQNEALALMLESRGVRVVNPAAVMGRCGDKLATTLALEAHGVPQ
                     PAWRTAFSSEGALAAAEELAYPVVFKPRTGSWGRLLAKANDREAAEAVVEHKAFLGAA
                     HATFYLQAYVEKGGFDVRAFVIGGEPRCAIRRESSHWITNTARGGRAANLPLDDDLTA
                     LLRRVHGAVGGEFLAVDLFSTAEGWLVNEVNDGGEFRNSMDPTGVDIAAEVVRCAWAA
                     RPEGL"
     gene            complement(7073..7243)
                     /gene="lysW"
                     /locus_tag="PL162_05135"
     CDS             complement(7073..7243)
                     /gene="lysW"
                     /locus_tag="PL162_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019878 - lysine biosynthetic process via
                     aminoadipic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine biosynthesis protein LysW"
                     /protein_id="HQF91440.1"
                     /translation="MANGVCGICDGAVALPDGTVAGEILRCPDCGTELEVVSMDPLRL
                     EEAPQVQEDWGE"
     gene            8032..8625
                     /locus_tag="PL162_05140"
     CDS             8032..8625
                     /locus_tag="PL162_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91441.1"
                     /translation="MSLSVETLPSPGAAWYTPPPPRRDPAEALSEGAPEKPSLDPARR
                     RASGEEGGVEARSPSQRPSAPERPSPTSGGVQALQEDRMKKRLGLEPCETCAHRRYQD
                     ESGDGSVSFQSPTPIAPEAAASAVRAHEYEHVRNEQVYARQEGREVVSQSVRIFTAVC
                     PECGRVYVSGGETRTTTASSPPKAPATPPESPFELVA"
     gene            8756..9448
                     /locus_tag="PL162_05145"
     CDS             8756..9448
                     /locus_tag="PL162_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HQF91442.1"
                     /translation="MRFVVEDALFHRLPLVCFGVVVARGVDNALPREDTTALLAEELR
                     RARERLAGRRARELPEISPYREAFTALGINPNKFPCSIEALLTRIEKGKDLPSINPLV
                     DLGNAISLRYALPIGAHDLGAAPGDVQVRFSRAGDRFVPFGESVPEFLDAGEPIYAVG
                     DAVKTRRWIWRQSEQGKVTEHTREVFFPIDGFSEGLLKSAQKDLAEALSRIFGVSAVT
                     GFVDAASRSFEG"
     gene            complement(9491..10258)
                     /locus_tag="PL162_05150"
     CDS             complement(9491..10258)
                     /locus_tag="PL162_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HQF91443.1"
                     /translation="MKKSAAFCGTWLGVTIFSLFLLMGPSGAHGNEKIVKLSSLEWPP
                     YIGAEMENQGYVAEAVRAAFERRGYRVEIEFFPWNRAVAMAHDGKVDGYFPEYYSDEV
                     AKDYILSEKFPGGPVGFFKRKDKDISFAKLEDLKGYCIGVVEGYVNTAEFDAASYLTK
                     DESVDDLMGLKKLLAGRTDLFFCDKFVGNYIGKKNFPDQIAEIEFMDPILEDKPFYIC
                     FSRATPDGQRIAEDFNKGLAEITADGTLEALRRKGGY"
     gene            complement(10463..12064)
                     /locus_tag="PL162_05155"
     CDS             complement(10463..12064)
                     /locus_tag="PL162_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQF91444.1"
                     /translation="MRKLSWRGWRRGVLLAAAVGLLAVGGAMAGEKVLTIAGFDTDTV
                     DPQMTTAEYFVPLNVFDRLVQVEPGSNPPRLVPMLAESWEISEDGKVFTFHLRKGVKF
                     HNGDDFTAEDVAYTINRLLDPATKSVQTDLFDTLVGARERLEGKADAVPGIEVLDAMT
                     IRLTLREPYAPFLAALATPGASIFSKKFTEPRSEKFGLAAEDTCGTGPFVLKEYALKD
                     HFTLDANTEYWGGRPKLDKLIARIVPDAETLRMMFLTGEIDVFDCDQGFSQIPFFMQD
                     PQWKDHIQKGPRVGIVYIAMNNQMKPFDNPKVRQAFQMAVDRQKILDTVYFGNGTLVN
                     GVIPRGLVGYNPAALPEIEYNPAKAKELLKEAGYPDGCDIVLTQVTGWSNRWVQINEL
                     VQQMVKAAGFNATLEAVDSATYYARRKEGKVMAYPQIWSLDSNDPDGTFYAFFFKNYS
                     FARSIAYNNEAAMNKIEELRFMADPVKRLAEYNALEKTIVHDDAAWLPLFSVDHLYVL
                     NPRVKNFVVPWNGWSDMSYHLMDVE"
     gene            complement(12164..13099)
                     /locus_tag="PL162_05160"
     CDS             complement(12164..13099)
                     /locus_tag="PL162_05160"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HQF91445.1"
                     /translation="MRKGRRLKPGDTVGLLSVSNPVDPREREACVQAVEALGFRTVCG
                     ESLFGGRGYLAATDEIRARDVNRMFSDSAIDGIFCVRGGEGANRLPELIDLEVVRANP
                     KVFLGYSDVTVLHLMFGGTGELCTFYGPMPHTEFRKSTFPGYVRDHLLRALTSPEPGG
                     EILPPPGDPPLRALRPGRAEGPLVGGCLSLISALMGTPWEMDTKGKVLIFEDVDEEPY
                     KYDRWLCQLRSAGKLDACVAVVVGQCTNAEPRHPERSLTLEEVFQDLLGPLKVPVLLG
                     VPFGHGARKATYPLGARALVDGDEGRFYLTESGVS"
     gene            complement(13228..14202)
                     /locus_tag="PL162_05165"
     CDS             complement(13228..14202)
                     /locus_tag="PL162_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQF91446.1"
                     /translation="MSDALVSVRHLTVSFRAEGGFFGRSHRRVRAVDDVSLDVEEGSI
                     FALVGESGCGKTTLGRALLRLLPPDSGEVLFRGKDLARMAPEELRRQRRRMQIVFQDP
                     YASLNPRMRIRDTLAEPFWTHEPHLSPREVEVRGRELLRLVGLRGEVLGHFPHQFSGG
                     QRQRIGIARALALHPEFVVCDEAVSALDVSIQAQVLNLLVDLREKFSLTYLFITHNLA
                     VVRHLADRVGVMFLGRVMEVGPTEELFSTPLHPYTKFLIGAVPLPDPRRRDRERLLLQ
                     GDVLSPTDLPPGCRFAPRCPYARDRCREEEPRMSEREGRKVACHYPLA"
     gene            complement(14199..>14712)
                     /locus_tag="PL162_05170"
     CDS             complement(14199..>14712)
                     /locus_tag="PL162_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQF91447.1"
                     /translation="GLRQRVMIAMALICSPKLLVADEPTTALDVTIQAQVLDLMMGLK
                     NRLGTAIVLITHDLGVVAETCDDVYVMYAGHIVEQGDVYRLFERPGHPYTQGLLRCVP
                     DASKPGRRGPLASIEGAVPLLTALPPGCRFAPRCPWAQELCHRQIPDLVAVGEGHLVR
                     CWRAEEGGKP"
     assembly_gap    14713..14722
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14723..15240)
                     /locus_tag="PL162_05175"
     CDS             complement(<14723..15240)
                     /locus_tag="PL162_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQF91448.1"
                     /translation="MDRGNAVLQVRNLRTEFATRRGIVPAVDGVSFEVFEGKVTGLVG
                     ESGCGKSVTALSILGLVTSPPGRVARGEILFEGRDLARMTPREMGAFRGRDLSMIFQE
                     PMTSLNPVKTVGNQVGEPLRVHLGLSAREARRRAEEMLDLVGIPEPQLRYGAFPHQLS
                     GGQRQRVLLMLAL"
     gene            complement(15252..16100)
                     /locus_tag="PL162_05180"
     CDS             complement(15252..16100)
                     /locus_tag="PL162_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQF91449.1"
                     /translation="MAGETLWGDVVRAFWKNRAALAGVGVVTVIALMALGAPWFLRGD
                     PYAIDLDLRHLPPSWDHWFGTDHYGRDLFTRVVHGARISLVIGLIPAFLSTVIGAVLG
                     VVSGFYGGKTDFWIMRLADVVLAFPSLLLAMAIMYTLGASLFNIFIALSAVGWASTSR
                     IVRSQTLSLREKDFVEAARALGVSRGKIVFRHILPNCLPSIVVVFTMSIPAAIMQEAG
                     LSFLGVGAQPPTPSWGLLVSQGKEFIFSAPWEAILPGVAILLLVLAFNFMGDGLRDAI
                     DPYMKD"
     gene            complement(16172..17107)
                     /locus_tag="PL162_05185"
     CDS             complement(16172..17107)
                     /locus_tag="PL162_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQF91450.1"
                     /translation="MGGFIVKRLAVSVPVLLGVTFLIFMLLHVVPGDPVALMMGERMS
                     PEVIDRVRAQMHLDDPWPVRYVRFISDALRGDLGESYKLRRPVVGLLRDAFPTTLKLA
                     LAAALVAWCIGIPAGILSAVRPYSLVDNFFMGLALTGVSTPVFWTALLFQYLFSLKLG
                     WLPVAGFYSAKHLIMPALVLGWSSSGLVARLTRSSLLDVMRHDYIRTARAKGLRQGPV
                     ILRHALKNALLPVVTVMALQIAFLLSGSVITESVFNIPGVGRIAVGAIQNRDMPLLQG
                     AVLFTTVLVILGNLAADLLYGWLDPRIRRDGGVGR"
     gene            17412..18320
                     /locus_tag="PL162_05190"
     CDS             17412..18320
                     /locus_tag="PL162_05190"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HQF91451.1"
                     /translation="MTSKRRVAVVGSGHVGREALAAIRETPDLEAAGVVLRDPARLEA
                     FSREVEAPTALDVDSLGPVDVAVLAIASRAVPEVAPSYLARGICTVDSFDIHGDAAME
                     LRQRLDVAGRGGHAAAVICAGWDPGTDSVVRALLECIAPRGITYTNFGPGMSMGHTVA
                     VKALPGVKDALSLTLPQGTGIHKRHVYVTLQEGADFEEVRGAILADPYFRHDECHVFP
                     AEDIRSLLDLGHGVHMERKGVAGRSHNQRMEFSMSVMNPAATAQVMVSAARAALRQKP
                     GSYTMLEIPLLDFLPGDRDALLRRLV"
     gene            18426..20126
                     /locus_tag="PL162_05195"
     CDS             18426..20126
                     /locus_tag="PL162_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HQF91452.1"
                     /translation="MKTAQIILEMFRRYGVDTVFGLPGETTLPLYDAWRSYPDLRHVM
                     ARDERSSVFMAHGYARVSGRPGVCEGPSVGAPHMVPGLLEARKAALPLIALTTDIPLH
                     MERRNMLTGFDQTALLASVVKESLTITRPEDAPFLVRRAFRLATSGRPGPVHLRLPMD
                     VASQEGSPDDLYAQDAFRACPGVRSAAAQGDVEAAVELLKASSQGVIVAGQGAVTSGA
                     WEELRLLAERFGLAVGTTLGGKGALAETHPLALGVVGSRGGTPFSHSVLAEADLIFFV
                     GSSTDSAGTAGWTRPPRHGKARVIQLDVSEAELGNNYRADLFLLGDARETLAALLRAS
                     DPAERHPGMRRRERREEGLALRRKAFLREDAAEGCPQGAPVHPFRFLQALERRLPEGT
                     TITVDPGISAIYPATYLRLKTPGRHFVSNFAMGALGFALPAALGASFADPGGPTVALS
                     GDGSLGFCAGELETLGRTGAPVKLFVFDNGTFGWIRATNLVDLGTGEVMATTFSPTDH
                     GRVAEGFGVPALTIATAEDVDSAVDEAFDRPGPMVVVVKVLSEERCLPPVPGWERAQG
                     "
     gene            20270..21913
                     /locus_tag="PL162_05200"
     CDS             20270..21913
                     /locus_tag="PL162_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQF91453.1"
                     /translation="MMKRLRLLLLSMAILGILATAGWGEAPIPGGVLSYRLASSPLTL
                     DPAQATDESSLRGVNLIFETLVDYDSDARQVTPLLAETWETNADATVWTFHLRRGVRF
                     HKTCGGEPTANGGREVRAQDVKYSLERLVRVRAPRVHFLDMINGYQDFASSDVTGVND
                     WKGIKVLDDHTLRFDLAYPFAPFLAALAYDSCGVVPSEDAEKWGKDFGLHPVGTGPFA
                     FKVWDSGVRLSLEKNHSYWMKDTLGHPLPYVDGVDLVIEPDDLLAYEGFKGGSIDILP
                     GCPDEAYQEAKDTLGPLGLFQQRPGMGDVYWCFNLTKEPFKDNLKLRQALNYAIDRRA
                     ISESVVKGRYCVAAGVLPPSVPGYNPRIRGYSFDLERARSLMKEAGYDKGLALTVHIA
                     ANARQRAVAEAIQAQLAPLGIALHIQESSGAAHRTLVRQGETALSWNSWMADYVDPDN
                     FLHVLFHSTNHGSRGNYSRYANPEVDRVLDEARKEMNWGRRIALYRRAEQLILDDAPW
                     IFLYHYTTDIMYRDAVHGVKLAALGEYKTKLTTTWKDPR"
     gene            complement(22563..24218)
                     /locus_tag="PL162_05205"
     CDS             complement(22563..24218)
                     /locus_tag="PL162_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQF91454.1"
                     /translation="MRGRMNTWVLGIWALAVLWGSGEAEGSCSIKAVAALQAKAAEMG
                     VVRVIVGLGADFRPEGDIGPGRAFVQQNGIATAQGRVLARLGNKLAGSPVTKFRTIPY
                     MAMQVSAEALKALLVDPDVISVQEDGLSKAYLNDTIPLIHAPAVWSEGQGGSGWCVAI
                     LDSGVDKSHPFLAGKVVSEACYTTTDSGQDCQSVCPGGAAESTAANSALPPLGGTYGT
                     RFYHGTHVAGIAAGSVPTMHGVARSADIIAIQVFSWFPNYGGQGKGDALSWASDQIRG
                     MERVYALRNTYKIAAVNMSLGSEKHASVCDKDARKAIIDNLRSVGIATVISSGNDGYS
                     NATGTPGCISTAVTVGATNKYMNLAPFSNIAYFVDLLAPGVSVWSSIPYDLYAPIDGT
                     SMATPHVTGAFAVLKAAYPYLSVAQIEDGLKDKGIKVKDGRPGTYYESSQFIQLKETY
                     DSFKLPFPSIFPTVTPMPIPSYVPIITKQPTLAPVPTIHWPTVAPTTPPTGSPTAAPT
                     GNPTGNPTVAPTGGGGGGGGGCSAGPVSGILLLALPLVILWRR"
     gene            complement(24630..>25192)
                     /locus_tag="PL162_05210"
     CDS             complement(24630..>25192)
                     /locus_tag="PL162_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91455.1"
                     /translation="YRFLVLRSPAALSDAEKPDVCQLTFEEMAAHIDDLEQAHPRVRK
                     LHLTVLDGKIWKIFAIVTNLHDMPGEDVIRWQRARCGRAEEMHAILKNDLAGGHVPSA
                     SFGASAAWWHLTVLALNLQKVLLLHVLPSGWGRVRQKRLLATLYAIPGRISRHARKTI
                     LTPAGHFAHIAEEALKALPQFCENTA"
BASE COUNT         4520 a         8975 c         7578 g         4109 t
ORIGIN      
        1 cctgcgagaa gaaggcagtt tctgcacatc tttgggcaaa gccgaatcaa ccactggtgt
       61 gaggtttttg gtctgcgcct taaccttact tccgctgttc aagcggcacc agaggggttt
      121 tgcgtgtctt ctcttcctgt tacctgcgga tcaaggcggc ccttaccctg ttgacagcat
      181 cgggaaggcc ctgtataatc ctttccgtcg ttgaagctcc cctgcgggtc cgtccaccgg
      241 caggggggtt ttttttggaa agactccgga aggtcacccc ccaaaccttt tttaagaaga
      301 ccgtcccctt tgacgacaga agtcccccga ggggggaccg caaaattatc aatttccgct
      361 ccgagcggaa agaaaggaag tttcatgtac agcaacaaac tcgtgtatat cggtaccacc
      421 ccgtactgga agaccccgga ggggcgcttt gagccggtgg aatgccgcga aggccgcatg
      481 attgaccggg tattcctgcg caccccccag gggctccgcc ccatggaaaa cggctgttcc
      541 cccgtctaac aaggaacaca tgtcccggag gcccctcgcc tccgggtttt ttcttttccc
      601 ccaccagatc cctcgggagg agagaagtac gaccggccac caccgggact ctgcgcccgt
      661 cgccctgagc ggcacagcgg acgtggcacg gatgtgagga ggcccccttc gcgctctccg
      721 caccaagggt cttttctccg tcccccaggg gcctccaggg agcaggacgc cgcaagagcc
      781 gcgttgcccg ccatgagccc cttctcccag cgttcatgcc cgacaccggg gacgtcttct
      841 ggatcatgcc tcctcccgcc acagccgggg cagggcctcc tgtagcaccg ccacggcggc
      901 ccggaactcc tccagcggga ggcgctcctc cgccgtgtgg tccagacggc tgtctcccgg
      961 accgtaggcc gccagaggac atccccagga cgccgccagg ttgaaatccg ccgtgcctcc
     1021 cttggccagc aggcggggcg ctcttccaaa aacctggcgg atggcccccc gcagggcccg
     1081 gaccgcgagg tcatcccgag ggaccaggtg agccctcact ttttccgaaa aatataccgt
     1141 cacgccccag cgctccgcca cctcccgcac ttcctggacc accctttcgg gctccgctcc
     1201 caggggaagg cgcagatcca gctccactcg cccccggcgc tctccctctt ctctcccctc
     1261 catgaaggcc accgccccgg aggggcgctc gatcaccggc cgggtggggt cgtccccccg
     1321 ctcccaggaa tccagcaccg ccgccgcccc ccggaggcac gccgtgtggg gccccgcatc
     1381 gccgctgcgg tgcgcccccc catcccgggc ctccagggtg cagaaaattc gcccccggta
     1441 ccccagggtc accccgtcgc ttcccgaagg ctcccccacg agacaggcct cgggggcgtg
     1501 ccgcatcagg cgataccggg ccccccggga atcccgctcc tctcccaccg cccccaccag
     1561 ggtgatacgt cccccctcgg gcacggcaca ggctcccccc gccagggcga aggccccgaa
     1621 ggcccccttg gcgtccacgg ccccccttcc ccagaggacc tccccttccc accgcacggg
     1681 gggccccccc ggcacggtgt ccacgtgccc cagaaggagc acttcccgag gtcccttgcc
     1741 ccgggaggcc accacgttgc ccacgtcgtc gagctccacc cgttcccacc ccagggccgg
     1801 aagacgggag accaggagcg cgccgatttc ctgctccgcc ccgctggggc tcgcacagcg
     1861 caccaactct tccagaaaga tccggtcacg atccacgaga aagctcccgc aacgcctctc
     1921 ccagggccgt cgccgcccgc agaaaacttt ccggagacgc accgaagggc ggaagaaagc
     1981 gcaccacccg ggggcccgcc ggcagggcca gcaccccccg gcgctggagt tcccgcacca
     2041 ccgcccccga ttctccttcg atctccaccc ccaccaaaag cccccgcccc cgcacctcgc
     2101 cccgccccgg ggcatccacc cgactcaact cttcccggaa cgcccggccc cgctcctcgg
     2161 cccaccggaa aaggtcgtgg cggtgcagta gctcccacgt ggcctctccc agggcgcagg
     2221 ccagggggtt agccccatag gtggagccgt gcccctggac gggaaagtcc cccagatttt
     2281 tccgccacac cgtgaccccc acagggaacc cccccgccac ccccttggcc agacagacca
     2341 cgtcggggtc gagccccgcc ccggagctcg cgagcaccgc gccacaccgc ccccagccgc
     2401 tctggatctc gtccgccacg agcagggccc ctgtctcccg gcaccgggcg gaaagggccc
     2461 ctccgaaggc cggatccagg gggtgaactc ccccctcccc ctgcaccggt tccacgaaga
     2521 ccgccgccac gtcgtcctcc accgcttcaa ggagcgcttc ggaagtgtgg tgttccacgg
     2581 cacagagctg ttcctgccag ggcttccgat atttcgggtt aaaggtgagg gccagggctc
     2641 ccagcgtccg cccgtgaaaa gagcgccgca gggccagaag tcgctttttc ccggggcgca
     2701 gggcgatgac gagcttgagg gcggcctcca ccgcctcggc gccgctgttg gaaagaaagg
     2761 agcacccctc ggggatcagg ccgtccagca gggagagaaa tcgggagcgc gccggatggg
     2821 aaagccccgc ccccagggtc cagcacgacg ccgccgattc ccggagcacc gaaaccagct
     2881 ccggatgggc atggccaaag agcgccgccc cgtgccccgc gagaaaatcc acgtattcgc
     2941 gcccttgggc gtcccagagc cgatctccgg ctccccggac cgccgtcaga ccgcgagagc
     3001 cgtagcccgc gctcggcata gctccgttcc cctccccgcc aacacctctt ccagcggacg
     3061 acgtccccga ctgtccccca gaaggacccg gggcactcct ccctccaggg cctcccgaca
     3121 ggcctggagc ttccgcttca tgttcccccg ggccatctct tccagttccc cccaatcctc
     3181 cagatccccc gaagccacca gagaatcggg gtcctgggga tcccgcaaca gtccgggcac
     3241 gttggaaaga atcaccaggg cctccgcgcc gagggccacc gccacggcgg cggcgaggcg
     3301 atcgccgtcc acgttcagat ttcgcccgga cacgccgtcc tgggccagcg gcggcaacac
     3361 gggaatttct ccccggctcc aggcgcgccg caacggcgcg gggtccacgt ggcggacggt
     3421 gccgctccag ttcccccgca caaggcggca ccgccccgat tccaccgccc ggagcacctc
     3481 cttgcgttcc cccgtcaccg atccctcccg gggccacagg gaaacccccc ggacgcccaa
     3541 ctctcccagg ctctgcacgt gctccaggga gacccgggcc gccgccgcct cgaagagctc
     3601 ccgttccccc tcccccacga agcggctccg gaagcccgaa ggactcacca catggcgcac
     3661 ctcgactccc gccgagcggc acaggccgtc catcacgccg ctggcgccgt gcaccagaac
     3721 ccagcgctct ccccggccca cccgctccgc cagatcccgc agcacgttct ccgccccgtt
     3781 tcccggcgca ccgccgatct tcaccacacc gaacatggcc actcctcctc ccgccccgtc
     3841 aggccggata gaccgccagg gcgtcgagcc ccgccgtttc ttcaaatccc agcatgagat
     3901 tggcgcactg caccgcgctc cctgcggcgc ctttcatgag gttgtcgatg gcacacccca
     3961 ccaagagccg cgttcccccc tcgtccaggg caaagcccac caaggctcgg ttggagccga
     4021 gcacccgccg aggatcggga aagcgcagat gggcggggcg ggcgggacag agctccacga
     4081 agggctcccc gccgtaggcc tctcggtaga ggcgccacag agccttttct tccatcgcct
     4141 ccgtcagggt cacgtgggcg agcatctggg cgccgcgcac catttccgcc gccgtcagcg
     4201 tgaaggaaaa ggccgaaagg ggcatccccg tctcctgggc cacctccgcc gcgtggcggt
     4261 gcctgaaggg ttccaccacg cgcagcgccc cccggcggta ggggtggtgc gaccccacgg
     4321 tgggcgtgcc tcccccttcg gaggaccccg ctcgcacctc gatgcgggcc tcccggatca
     4381 acccccgggc cgccaaagga cgtagtccca ggttcgtgca ggtggcgttg caccccactc
     4441 cgctggccac cggggtttcc cggagggctt cccgatggaa ctccggcagg ccgtagaccg
     4501 cccgggccag aagatcgggg cagggatggg gagtgccgta ccagcgacgg tggtcctccg
     4561 gatccctcag gcgaaaatcc gccgacatgt cggccacccg cactcctcgg gcaacgtgga
     4621 acgcggcgat atcggccccc gcgccgtggg gcaccgccag aaagaccagg tcaacctcct
     4681 cggccctccg ccgggcctcc tccggggagg aaaagaccat gtcgcccaag gcgtgtcgca
     4741 ggtgggggtg ctggtgccac accggttccc ccgcgtgcct cgccgacacc gcccgcaccg
     4801 tctccatttc cggatgtcgg gccaaaagcc tgagcacctc gccccccgcc atccccgaag
     4861 ctccccagat ccccactcgc ctcatatcgc ccgctcctcc ttcaaccctt cgatggtccc
     4921 ccgacgaccc ttggtcgccc ctcgccaaaa gcctgagaag gcctccctag gaacgctccc
     4981 cctggggctc ccgatggcgc cttatctccc tcgcctcgcg ccttccggag accctaaagc
     5041 cccccccggg ggcgcgcccc aacccttccc gaaggggaga ggactcctcc cccacctccc
     5101 ggagggcata tcggcgaaag gaccctccgg aggtctcccg caggatgtgg gccgtgagcc
     5161 ccggcagcct tcccgccaga aagaacgccc gccccgcctg gaagggaaag cccaggtcgg
     5221 aaaggacggc gccgcagatc ccatccacgt tccccccgat gccctttcgc tcccccagga
     5281 gatcctccat ccgggaaaaa aacgccaggt ggtcccgccg aagggagcgc tcctccgcca
     5341 ggcaccgcag gggggccacc cgggggtccc ggcgatgcag gcggtggccg aaaccgggaa
     5401 cccggcgccc cgacgccagg gcctcgtcca ccagcgcctc ggggtcgaga ccccgggcca
     5461 ctccgctctg gaggatttcc atgaccccct ccagagcccc cgcgtggtga ttcccgaagg
     5521 ccaaaagccc cgccgccagg ctcgcctgaa gaggaactcc cgtggaagcc accaggcgcg
     5581 ccgcctgggt gctcggagga gtgtccccat ggtccgccag ggtggcgagg accgcatccc
     5641 acagccgccg ttccccctct ccggggagcc tcccctggat caggcgaaac cacacctccg
     5701 ccggagaaac ctcggcgatc catcgctcca ccgccgtgcc gtcaaaggcc agatatcctt
     5761 ctcccacctc cgtgggacgc cccctcacca ccattcccca tccctcccca ggtgctcttc
     5821 cggcaaaagc ggaagttccc ttctcccgcc gcactcttct cggccgacct cttcgtttcg
     5881 tcgcgctcgg acggtgatgt cgatctccag atcccccgac gcctctccgg caaacagccg
     5941 cacgataccc atgtcccttc ctccccttct ttccggcgcg ccccaccgtt tcccggggcg
     6001 acaagaaaaa aacccgcttt ccgaagacgg aatgcgggcc gtgggtttcc tccgataaac
     6061 ttatgcgaag tgttttaaac ctcagaacac tccgccggat gccacgaccc aggatggcgc
     6121 cccgctctgc ggagccaccg tgcgtttgga acgtttcaag aaaacgcgcg ccgaaacggt
     6181 ggtcatcgtc attcctcctc ttcctgccgc tctcttttcc cgatcagagc ccttcagggc
     6241 gcgccgccca ggcacaccgc accacttccg ccgcaatatc cactccggtg gggtccatgg
     6301 agtttcggaa ctcgccgccg tcgttcactt cgttcaccag ccacccttcg gccgtggaga
     6361 agaggtccac cgccagaaat tctcccccca ccgccccatg cacccggcga agcagggccg
     6421 tcagatcgtc gtccaggggg agattggccg cccgccctcc ccgggcggtg ttggtgatcc
     6481 agtgggagct ctcgcgccgg atggcgcacc gcggctcccc gccgatgaca aaggcccgga
     6541 cgtcaaagcc ccctttttcc acgtaggcct gaagatagaa cgtggcgtgc gccgccccaa
     6601 gaaaggcctt atgctccacc acggcctccg ccgcctcccg gtcgttagct ttcgccagca
     6661 ggcgccccca gctccccgtc cggggcttga acaccaccgg gtaggccagc tcctccgccg
     6721 ccgccagggc cccctccgaa gagaacgccg tccgccacgc cggttggggc acaccgtggg
     6781 cctccagcgc cagggtggtg gcaagcttgt cgccgcaacg ccccatcacc gccgcagggt
     6841 tcaccacccg caccccccgg ctctccaaca tcagagccag cgcctcgttc tggctgtggg
     6901 agatgcaccg agccagcacc acgtccccct gcgcggcggg aagccgctcg ccgaagaccc
     6961 catccgtcac gtcctccagg gtggcaggaa tcccctcccg ggaggccgcc gccgccagaa
     7021 gtttctcttc ggtccgaaga cgagagtaca aaatccgcaa ccgggccacg gtctactctc
     7081 cccagtcttc ctgcacctgg ggagcttcct ccagccgcag aggatccatg ctcaccactt
     7141 ccagctctgt gccacagtcg ggacaccgca aaatttcccc cgccaccgtc ccgtcgggaa
     7201 gggccaccgc tccgtcgcag atgccacaca caccgtttgc catcacgtat tcctcctttg
     7261 cgggcgcacg cacgccctct ttgtgcataa aaaaatcggc tcgccccctc atcagggacg
     7321 agccgataga cccgcggcac caccctgctt ggatttccgc cgtgtgtcgg aaatccccgc
     7381 tcgagagccc atgacggagg gcagaaaccg ggctgcgtac tccggggaga tctccccttt
     7441 ccgcgccagc tccccgagcc ttgggccttc cgaactcttc cgagggggcc ttgcagccgg
     7501 tgagccccca tctctggcgg aagaggcctc cggggcctct cggatcatcg ctttttctct
     7561 tttggtccga ccaggagcct ccccccggac gttggaacgc attataggca ccgatcgaaa
     7621 aagcgtcaag ggggggaaat ttttttgtgc cgggatttgg accccgtcac ccctgaagag
     7681 accccggatc cgcttctggt cccgcatcgg cccctcgcag aaggcctttt cggggcccgc
     7741 ctcgctccct ttcggaaggc ccggcacggg gggacaactc ttttatcccc gcgccgaacc
     7801 cttcgccgca acggcggccg ggagttccgg gggagtccgg agcatcgggg agggcccgct
     7861 ggcgccgaag tagggtgcga cggatcgctc ttcccgcccg gcgagcgccc ctcgctctgt
     7921 ggccgagagg ggaagatcga gaccggaccc cgaggactcc ttgctttttt tccgcaaaca
     7981 cgctacactc atcatggata ttgctctacc cgaagaaagg aggcgagacc catgtccctt
     8041 tccgtggaaa cccttccctc tccgggggcg gcgtggtaca cccctccgcc cccgcgaagg
     8101 gatcccgccg aggccctctc cgaaggagcc ccggagaaac cctccctgga tccggcccgg
     8161 cgaagggctt cgggcgagga gggaggcgtg gaggcccgct ccccctccca gaggccttcg
     8221 gccccggaac gcccctcccc gacctcgggg ggtgtccagg ccctgcagga ggatcgcatg
     8281 aaaaagcgcc tggggctgga accctgtgag acctgtgccc atcgccgcta tcaggacgaa
     8341 tcgggagacg gtagcgtgtc tttccagtcc ccgaccccca tcgccccgga ggccgccgcg
     8401 tcggcggtgc gggcccacga gtacgaacac gtgcgcaacg agcaggtcta cgcccgacag
     8461 gagggacgag aagtggtgag ccagagcgtg cgcatcttca ccgccgtatg ccccgaatgt
     8521 ggccgggtct acgtctccgg cggagagacc cgtaccacca cggcatcgtc tccgcccaag
     8581 gcccccgcca cgcctcccga gagccccttc gagctggtgg cgtagccccc cgaggcgccc
     8641 cgaagactcg ccgcccctga agggcggcgc tttttttgcc ccacaggcgc catcgtggta
     8701 ccatgggcgg atacggacct cttcgggaga tccgcaaaga aaggacggga actccatgag
     8761 atttgtggtg gaagacgcgc tgttccacag actacccctg gtctgtttcg gcgtggtggt
     8821 ggcgcgaggc gtggacaacg cccttccccg ggaggacacc accgccctgt tggcggaaga
     8881 gctccgccgg gcccgggagc ggctcgccgg acgccgagcg cgggaattgc ccgagatctc
     8941 cccctaccgg gaggccttca ccgccttggg gatcaacccc aataagttcc cctgctccat
     9001 cgaggccctg ttgacccgga tagaaaaggg caaggacctg ccctccatca accccttggt
     9061 ggacctgggc aacgccatct ccctgaggta cgccctgccc atcggcgccc acgacctcgg
     9121 cgcggccccg ggggacgtcc aggtacgctt ttcccgggcg ggggaccgct tcgtgccctt
     9181 cggggagagc gttccggagt tcctcgacgc cggagagccg atctacgccg tgggagacgc
     9241 ggtgaagacc cggcggtgga tctggcgcca gagcgagcag ggcaaggtca ccgagcacac
     9301 ccgggaggtc tttttcccca tcgacggctt ttccgagggt ctcctgaaga gcgcccagaa
     9361 ggacctggcc gaggccctct cccggatctt cggcgtctcg gcggtcacgg gctttgtgga
     9421 tgccgcctcc cggagtttcg agggatagcc ccccggagtc cggagggcca tcccgtcaaa
     9481 tccgggccgc ctagtatcct ccctttcggc gcagagcctc cagggtgccg tcggcggtga
     9541 tctccgccag ccccttgttg aagtcctccg cgatccgctg tccgtcgggg gtcgcccggg
     9601 agaagcagat gtaaaatggc ttgtcttcga ggatggggtc catgaactcg atctccgcga
     9661 tctgatcggg gaaatttttc tttccgatat agttccccac gaacttgtca cagaaaaaaa
     9721 gatcggtacg accagccagg agttttttta gccccatcaa atcgtcgacg gattcgtcct
     9781 tggtaaggta ggacgccgca tcgaactccg ccgtattcac atatccctcg accacgccga
     9841 tgcaatagcc ctttagatcc tccaatttgg cgaaggatat gtctttgtcc tttcgcttga
     9901 aaaaccccac ggggcctccg gggaactttt cagacaggat gtagtctttg gcgacctcgt
     9961 cgctgtagta ctcggggaag tatccatcca cttttccgtc gtgggccatg gccacagccc
    10021 ggttccaggg gaaaaactcg atctccaccc gatatcccct tctctcgaag gcggcccgaa
    10081 ccgcctcagc cacatacccc tgattctcca tctccgcgcc gatataaggc ggccattcca
    10141 gcgaagatag tttcactatt ttctcattac catgggctcc ggaaggcccc atcaagagaa
    10201 agagcgaaaa aatcgtcact cccagccagg tcccgcaaaa cgccgcagat tttttcatga
    10261 caaattcctc ccttgtccga tgttctctcc ccttaccacc ggcgtcactt cttcgcaaca
    10321 acgcaggccg atgggggggt ggcccggcgc ggcccaaatc ccttcgccaa gagaacccct
    10381 tggtgccctt ccattccttc cgggatagtt ccccgggaat catccgaggg gggcgacggc
    10441 cgtcaccccc ctcgggtgat ttttattcca cgtccataag gtgataggac atgtcactcc
    10501 agccgttcca gggcaccacg aagtttttca cccgggggtt gaggacatag aggtggtcca
    10561 ccgaaaagag ggggagccac gccgcatcgt cgtgaacgat ggttttctcc agggcgttgt
    10621 actccgccag gcgcttcacc gggtccgcca tgaagcgcag ctcctcaatc ttgttcatgg
    10681 ccgcctcgtt gttgtaggcg atggatcggg caaaggaata gttcttgaag aagaaggcgt
    10741 agaacgtgcc gtcggggtcg ttggaatcca gcgaccagat ctggggatag gccatgacct
    10801 tgccttcctt gcgccgggcg tagtacgtgg cggagtccac cgcctccagg gtggcgttga
    10861 agcccgcagc cttcaccatc tgctgcacca gctcgttgat ctgcacccac cggttggacc
    10921 atccggtgac ctgggtgagg acgatgtcgc agccgtcggg atatcccgcc tctttcaaca
    10981 attccttcgc cttggcgggg ttgtactcga tctccggcag agctgcgggg ttatacccca
    11041 cgaggccccg gggaatgacg ccattcacca gggtgccatt gccaaagtac accgtgtcga
    11101 ggatcttctg gcggtccacc gccatctgga aggcctgacg gaccttggga ttgtcgaagg
    11161 gtttcatctg gttgttcatg gcaatgtaga cgatgcccac ccgggggccc ttctggatgt
    11221 ggtccttcca ctggggatcc tgcatgaaga agggaatctg ggagaacccc tggtcgcagt
    11281 cgaagacgtc gatctccccg gtgaggaaca tcatgcgcag ggtctccgcg tcggggacga
    11341 tgcgggcgat gagtttatcc agcttgggcc gccctcccca gtactccgta ttggcgtcca
    11401 gggtaaaatg gtccttcagc gcgtattcct tcagcacgaa gggccctgta ccacaggtgt
    11461 cctcggcggc gagaccgaac ttctcgctcc ggggttcggt gaactttttg ctgaagatgc
    11521 tggctcccgg cgtggccaag gccgccagga atggggcgta gggctcccgc agggtcagcc
    11581 ttatggtcat ggcatcgagg acctcgatgc cgggcacggc atcggccttg ccctccaggc
    11641 gctcccgggc ccccaccagg gtgtcgaaga gatcggtctg aacggacttg gtggcggggt
    11701 ccaacagccg gttgatggtg taggccacat cttcggcggt gaagtcgtcg ccgttatgga
    11761 actttacccc cttccgaagg tgaaaggtga agaccttgcc atcctcggag atctcccagc
    11821 tctcggcgag catgggaacg aggcggggag gattggagcc gggctccacc tgtaccaggc
    11881 ggtcgaagac gttgaggggc acaaagtact ccgccgtggt catctgggga tccacggtgt
    11941 ccgtgtcgaa ccccgcaatg gtgaggacct tttctcccgc catcgcccct cccaccgcaa
    12001 ggagccccac cgccgccgcc aacaacactc cccgtctcca tccgcgccat gataattttc
    12061 tcatactttg tccctccact ctctgttttt tgggtctctt cgcaggaccc tccctcaccc
    12121 tcgccccggg cctcctttct ttcttagcat cgttgaatat tttttaactc accccggatt
    12181 cggtgaggta aaagcgccct tcgtcgccat ccaccagggc ccgggccccc agggggtagg
    12241 tggcctttcg agccccgtgg ccgaagggga cccccaggag cacggggacc ttcaagggac
    12301 ccaaaaggtc ctggaagacc tcttccaggg tcaggctcct ttcggggtgc cggggctcgg
    12361 cgttggtgca ctgccccacc accacggcga cacaggcgtc gagttttccc gccgagcgta
    12421 gctgacagag ccaccggtcg tacttgtagg gctcttcgtc cacgtcctcg aagatgagga
    12481 ccttgccctt agtgtccatc tcccaggggg tgcccatgag ggcggatatc agggacagac
    12541 acccccccac cagagggccc tcggccctgc cgggcctcag ggcgcgcagg gggggatccc
    12601 ccggcggggg caagatctca ccgccgggtt cgggggatgt aagggcccgc aggaggtggt
    12661 cccggacgta gcccggaaaa gtgcttttgc gaaactccgt atggggcatg gggccataga
    12721 acgtgcagag ctcccccgtc cccccgaaca tgaggtgcaa cacggtgacg tcgctgtacc
    12781 ccaaaaagac cttggggttt gcccgcacca cctccaggtc gatgagctcc ggcaggcgat
    12841 tggccccctc tcccccccgg acgcagaaga tgccgtcgat ggcggagtct gaaaacatgc
    12901 ggttcacgtc ccgggcccgg atctcgtcgg tggccgcgag gtaaccccga ccgccgaaga
    12961 ggctttcgcc gcacaccgtc cgaaagccca gcgcctccac cgcctggaca caggcctccc
    13021 gttcccgggg gtccacgggg tttgacacgc tcaaaagccc cacggtgtct ccgggtttta
    13081 gtcttctacc cttgcgcacg gagatccctc cttgcttcgc ccgcgcgaag tccttcggtc
    13141 caaaaagttc caccgccgga aggcctttcg ggcagaagtc cggccgatcc ccccggcggc
    13201 ccggcgcctc ccttccgccc cttttcccta ggccagggga tagtggcagg ccaccttccg
    13261 cccctcccgc tccgacatcc ggggctcttc ttcccggcat cgatcccggg cgtaggggca
    13321 tcggggggca aagcggcacc ccgggggcag atccgtaggg ctcagcacgt cgccctgaag
    13381 caaaagcctt tcccgatccc gccgccgggg gtcggggagg ggcacggcgc ctatgagaaa
    13441 cttggtgtag gggtgaaggg gcgtcgaaaa gagctcttcg gtgggcccca cctccatcac
    13501 ccgtcccaag aacatgaccc ccacccggtc cgccaggtgg cgcaccaccg ccaagttgtg
    13561 ggtgatgaaa agataggtga gggagaattt ctcccggagg tcgaccaaca gattcagtac
    13621 ctgggcctgg atggaaacgt ccagggccga taccgcttca tcacagacca cgaactccgg
    13681 atgcaacgcc aaagcccggg cgatgccgat gcgctggcgc tgccctccgc tgaactgatg
    13741 gggaaaatgc cccaggacct cgccccgcaa ccccaccagc ctcaggagct ccctcccccg
    13801 gacttccacc tcccgggggg aaaggtgggg ctcgtgggtc caaaagggct ccgccagggt
    13861 atcccggatg cgcatccggg ggttcaagga ggcgtaggga tcctgaaaga cgatctgcat
    13921 cctccggcgc tgccgccgga gttcttccgg agccatccga gccaggtcct tcccccgaaa
    13981 aaggacctcc cccgaatccg gaggcaggag gcgcaacagg gcccgtccca gggtggtctt
    14041 gccacacccc gattctccca ccagggcgaa gatggagccc tcttcgacgt ccagggagac
    14101 gtcgtccacc gccctcaccc tccgatgaga tctcccaaaa aatcccccct cggcccggaa
    14161 ggacaccgtc agatgccgca cggaaaccag cgcatcgctc aaggcttgcc tccctcctcc
    14221 gcccgccagc agcgcaccaa atgcccctcc cctacggcga cgaggtccgg gatctgccgg
    14281 tggcagagtt cctgggccca ggggcatcgg ggggcgaaac gacaccccgg aggcaaggcc
    14341 gtgaggaggg gcaccgcccc ctcgatgctc gccaagggcc cccggcgccc cggcttagat
    14401 gcgtcgggga cgcaccggag caatccttgg gtataggggt gcccggggcg ctcgaagagg
    14461 cggtagacgt ccccttgctc cacgatgtgt cccgcataca tgacgtacac gtcgtcgcag
    14521 gtctcggcca ccacccccag gtcgtgggtg atgaggacga tggcggtgcc gaggcgattt
    14581 ttcagcccca tcatgagatc caacacctgg gcctggatgg tgacatccag cgccgtggtg
    14641 ggctcatccg ccaccaggag cttggggctg cagatgaggg ccatggcgat catgacccgc
    14701 tggcgcaatc ccnnnnnnnn nnagggccag catgagcagc acccgctgcc gctgcccccc
    14761 cgagagctgg tggggaaagg ctccgtaccg gagctggggc tccggaatgc ccacgaggtc
    14821 tagcatttcc tccgcccgcc gccgggcctc cctcgccgag aggccgaggt ggaccctcaa
    14881 gggttctccc acctggtttc ccacggtctt caccggatta agggacgtca tgggctcctg
    14941 aaatatcatg gaaaggtccc tgccccggaa ggcgcccatc tcccggggag tcatccgggc
    15001 caaatcacgc ccctcgaaga ggatctcccc cctcgccact cgcccggggg gcgaggtcac
    15061 cagccccagg atcgaaaggg ccgtgacgct tttgccgcac cccgattccc ccaccagccc
    15121 cgtgaccttt ccctcgaaga cctcgaaaga caccccgtcc acggcgggga cgatcccccg
    15181 gcgggtggcg aattccgtcc tcaagttccg cacctggagc accgcgttcc cccgatccat
    15241 ggcgatttcc cctaatcctt catatagggg tcgatggcgt cccgcagacc atctcccatg
    15301 aagttaaaag ctagcacgag caggaggatg gccaccccgg ggaggatcgc ctcccagggg
    15361 gcggagaaaa tgaattcttt cccctgggac accagaaggc cccagctcgg cgtggggggc
    15421 tgggctccca cgccgaggaa cgaaagcccc gcctcctgca tgatggccgc cgggatcgac
    15481 atggtgaaga ccaccacgat gctgggcagg cagttgggca ggatgtggcg aaagacgatc
    15541 tttccccggg agacccccag ggcccgggcg gcctccacga agtccttttc cctgagagac
    15601 agcgtctggg atcgcacgat gcgcgacgtg ctggcccatc ccactgcgga gagggcgatg
    15661 aagatgttga aaaggctcgc ccccaaggtg tacatgatgg ccatggcgag gagcagcgaa
    15721 ggaaaggcca gcaccacgtc cgccagccgc atgatccaaa aatccgtctt ccctccgtaa
    15781 aacccgctca ccacccccag cacggcgccg atgacggtgg ataaaaaggc gggaatgagg
    15841 ccgatgacca aagagatccg ggcgccgtgg accaccctcg tgaaaagatc tcggccgtaa
    15901 tgatccgtgc cgaaccaatg gtcccaggaa gggggcaggt gacgcaggtc caggtctatg
    15961 gcatagggat ctcctcggag gaaccagggg gcccccaggg ccatcagcgc gatgaccgtc
    16021 accactccca cccccgccag ggcggcccgg ttcttccaga aggcccggac cacatccccc
    16081 catagggtct ctcccgccat gtcatcgccc gcctcggccg ggacaagggg ggggagttcg
    16141 tcggcgatca actttgggtc tcgacccggc ctcatcgccc cacccccccg tcgcggcgaa
    16201 tgcggggatc cagccagcca taaaggaggt ctgcggcgag attgcccagg atcaccaaca
    16261 cggtggtgaa aagcaccgcc ccctggagca ggggcatgtc cctgttttgg atggccccca
    16321 cggcgatgcg ccccaccccg gggatgttga agacgctctc cgtgatgacc gatcccgaga
    16381 ggagaaaggc gatctgaagc gccatcaccg tcaccacggg gagcagggcg tttttcaggg
    16441 cgtgccgcag gatcaccggc ccctggcgca ggcccttcgc ccgggccgta cggatgtagt
    16501 cgtggcgcat gacgtcgaga aggctagagc gggtgaggcg ggccacgagc cccgatgagc
    16561 tccaccccag caccagggca ggcatgatga gatgtttggc gctgtaaaac cccgccacag
    16621 gaagccaccc tagcttgagg gaaaagaggt actgaaagag gagggcggtc caaaagaccg
    16681 gcgtggagac ccccgtgagg gcgaggccca tgaaaaagtt gtccaccaag gaatagggcc
    16741 tcaccgccga caagatcccg gcgggaatgc ctatgcacca ggccaccagg gcggcggcga
    16801 gggccagttt cagcgtggtg ggaaaggcgt ctcgcagaag gcccaccacg gggcgccgga
    16861 gcttgtagga ttcccccaga tctccccgca gggcgtcgga gataaaacgc acgtaccgca
    16921 cgggccaggg gtcgtccaag tgcatctggg cccgcacccg gtcgatgacc tcggggctca
    16981 tgcgctcccc catcatgagg gccacgggat cccccggcac cacgtggagc agcataaaga
    17041 tcaaaaaagt caccccaaga aggacgggca cggataccgc taaccgcttg acgatgaatc
    17101 ctcccactcc aacccctccc cccctcgtcc acccctatcc ggggaccccg gcgccggaaa
    17161 agaccttcgc ctccggacga gcctcacccc gccggagccg tcgtcccagg gcggtagctc
    17221 atcatcggtg aggggcgtgg ccattctaag gccctctcca tctcctgtca accccgtcct
    17281 gccaactcgc cctcccgtcc tgccaacccg ccctcccgtc ccttcgtttc ttgacaacct
    17341 tccgggcgct gctactatgg cctcagcctc gatctttccc cctgggtgac aggagaaagg
    17401 aagtccttcc catgacgtca aagagacggg tggcggtggt gggctccggc cacgtaggcc
    17461 gggaggccct ggcggcgata cgggaaactc cggacctgga agcggcgggg gtcgtactcc
    17521 gagaccccgc ccgactggag gccttctcgc gggaggtcga agcccccacg gcgctggatg
    17581 tggattccct cggccccgtg gacgtggccg tgctggccat cgccagccgg gcggttccag
    17641 aggtggcgcc gtcgtacctg gcccggggaa tctgcaccgt ggatagcttt gacatccacg
    17701 gagatgcggc gatggagctt cgccaacgcc tcgacgtcgc gggtcgaggg gggcacgccg
    17761 ccgcagtgat ctgcgccgga tgggatccgg ggacggattc cgtggtgcga gccctcctgg
    17821 agtgcatcgc cccccggggc atcacctaca ccaacttcgg ccccggcatg agcatggggc
    17881 acaccgtggc ggtgaaggcc ctccccggcg tgaaggacgc cctctccctc accctgcccc
    17941 agggcacggg gatccacaag cgccacgtgt acgtcaccct tcaggagggg gcggatttcg
    18001 aagaggtccg gggggccatc ctggccgacc cctacttccg ccacgacgaa tgccacgtct
    18061 ttcccgccga ggacatccga tcgctcctcg acctgggaca cggcgtccac atggagcgca
    18121 agggcgtggc cggacgaagc cacaaccagc gtatggagtt ctccatgagc gtcatgaacc
    18181 ccgccgccac ggcccaggtc atggtgtccg ccgcccgggc ggccctgcgc caaaagcccg
    18241 ggagctacac catgctggag attccccttt tggacttcct gccgggggac cgggacgccc
    18301 tgttgcgccg cctggtctag cccctcgccc cggcgagaag gggcaccgca aacgccctcc
    18361 ccgtctcccg gggccgcgcc ttccctcccc ggaacgccga aaggatctcc ctgaggaggt
    18421 ttgccatgaa aacagcccag atcatcttgg aaatgtttag acgctatggc gtagacacgg
    18481 tcttcggcct gcccggggaa accaccttgc ccctctacga cgcctggagg tcctaccccg
    18541 acctccgcca cgtaatggcc cgggacgaac ggagtagcgt cttcatggcc cacggctacg
    18601 cccgggtttc ggggcgtccg ggggtgtgcg agggccccag cgtgggagcg ccgcacatgg
    18661 tccccggcct tctggaggcc cgcaaggccg ccctgcccct catcgccctc accacggata
    18721 tccccctcca catggagcgg cggaacatgc tcacgggctt cgaccagacc gccctcctcg
    18781 cctccgtggt caaggaaagc ctcaccatca cccggccgga ggacgcccct ttcctggtgc
    18841 gtcgggcctt tcgtctggcc acctcgggac ggccggggcc cgtgcacctg cgcctcccca
    18901 tggacgtggc ctcccaagaa ggctcccccg acgacctcta cgcccaagat gcctttcggg
    18961 cgtgccccgg cgtccggagc gccgccgccc aaggggacgt ggaggccgcc gtggagctcc
    19021 tgaaggcatc gtcccagggg gtcatcgtgg cggggcaggg ggccgtgacc tcgggagcct
    19081 gggaagagct gcgcctcctg gcggagcgct tcggcctcgc cgtgggcacc accctgggag
    19141 gcaagggggc cctggccgag acgcaccccc tggccctggg ggtggtggga tcccggggag
    19201 gcaccccctt ctcccacagc gtcctcgccg aggccgacct gatctttttc gtgggatcca
    19261 gcacggactc cgcaggcacg gcggggtgga cccgacctcc ccgccacggc aaggcccggg
    19321 tgatccaatt ggatgtcagc gaggccgagc tgggaaacaa ctaccgcgcc gacttattcc
    19381 tactgggaga cgcccgggag accctggcgg cccttctccg ggcgtcggac cccgccgaaa
    19441 ggcaccccgg gatgcgccgc cgggaacgtc gcgaagaagg cctggccctc cggcgaaagg
    19501 ccttccttcg tgaagacgcc gcagaaggat gcccacaggg cgccccggtg caccctttta
    19561 ggttcctcca agccctggaa cggcgccttc cggaagggac cacgatcacg gtggatcccg
    19621 gcatcagcgc catctacccc gccacctacc tccggctcaa aaccccgggg cgacacttcg
    19681 tcagcaactt cgccatgggg gctttggggt tcgccctccc cgccgccctg ggggcctcct
    19741 ttgccgaccc cggaggacct acggtggccc tgtcgggaga tggcagcctg gggttttgcg
    19801 cgggggagct ggagaccctg gggcgcacgg gggcccccgt caagctcttc gtcttcgaca
    19861 acggcacctt cgggtggatt cgggccacca acctcgtcga cctcggcacc ggggaggtca
    19921 tggccaccac cttttccccc accgaccacg gccgagtggc cgaggggttc ggcgtccccg
    19981 ccctgaccat cgccaccgcc gaagacgtgg actccgcagt ggatgaggcc ttcgatcgcc
    20041 ccggccccat ggtggtcgta gtgaaggtcc tgtccgaaga acgatgcctg ccccccgtgc
    20101 cggggtggga aagggcgcag gggtgaattc gatggggagg accccgcccc gccccccggc
    20161 tcgatgtgaa agaggcgcaa ggatgatcgc gatggtccct ttggagcgag cccccgggag
    20221 aagacccttc cggagcccca cggggcctga agggggtgaa gaggctggca tgatgaaaag
    20281 actgcgcctt ttgcttttat cgatggcgat cttggggatc ctggcgacgg cggggtgggg
    20341 ggaagccccc attcccgggg gagttttgtc ctatcgcctc gcctcctccc cccttaccct
    20401 ggatcccgcc caggccacag atgagagctc cctccggggg gtgaacctga tctttgagac
    20461 cttggtggac tacgactccg acgcccgcca ggtgacgccc ctcctggcgg agacctggga
    20521 aaccaacgcc gacgccacgg tctggacctt tcacctacgc cgaggagtgc gcttccacaa
    20581 gacctgcggc ggagagccca cggccaacgg cggccgggag gtgagggccc aggacgtgaa
    20641 gtactccctc gagcgcctgg tcagggtccg ggccccccgg gtgcactttt tagacatgat
    20701 aaatggttac caagacttcg cgagctccga tgtcaccggc gtcaatgatt ggaagggcat
    20761 caaggtcctg gacgaccaca ccctccgctt cgacctggcg taccccttcg ctcccttcct
    20821 ggccgccctg gcgtacgact cctgcggggt ggtgccctcc gaagacgcgg aaaaatgggg
    20881 aaaggacttc ggcctccacc ccgtgggcac ggggcccttc gccttcaagg tctgggactc
    20941 cggggtccgc ctttccctgg agaaaaacca ctcttactgg atgaaagata ccctgggaca
    21001 tccccttccc tacgtcgacg gcgtggatct ggtcatcgaa cccgacgacc tcctggccta
    21061 cgagggcttt aaagggggat ccatcgacat cctgcccgga tgtcccgacg aggcatacca
    21121 agaggcaaaa gacaccctgg ggcccctggg gctcttccag cagcgccccg gaatgggaga
    21181 tgtgtattgg tgttttaatt taaccaagga acccttcaag gacaacctaa agctccgtca
    21241 ggcccttaac tacgccatag atcgaagggc catcagtgaa tcggtggtga aggggcgcta
    21301 ctgcgtggcc gccggcgtcc ttccccccag cgtgccgggg tacaatccgc gaattcgcgg
    21361 ctattccttc gatctcgaaa gggcccgaag cctcatgaaa gaagcggggt acgataaagg
    21421 gctggcattg acggttcata tcgcggccaa cgcccgccag agggccgtcg ccgaggcgat
    21481 ccaggcccag ctcgcccccc tggggatcgc cctgcatatc caggaatcga gcggggcggc
    21541 ccaccgaacc ctggtacgcc aaggggaaac ggccctttcc tggaacagtt ggatggccga
    21601 ctacgtggac ccggataact tcctccatgt cctctttcac tctacgaacc atggaagccg
    21661 ggggaactac tcccgctacg ccaatcccga ggtggaccgg gtgctggacg aagcccggaa
    21721 ggagatgaac tggggaaggc gcatcgccct ctaccgccgg gcggaacaat tgattttaga
    21781 tgatgccccc tggatctttc tgtaccatta caccacggac atcatgtacc gcgacgccgt
    21841 ccacggagtg aagctcgccg ccctagggga gtacaagacc aagctcacga caacctggaa
    21901 ggatccccgg taggggcgga cccccggagg gcttcatgcc ccgcgacgcc gtccacggag
    21961 tgaagctcgc cgccctgggg gagtacgaga ccaagctcac gacaacccgg aaggatcccc
    22021 ggtaggggcg gacccccgga gggcttcatg ccccgcgacg ccgtccacgg agtgaagctc
    22081 gccgccctgg gggagtacga gaccaagctc acgacaaccc ggaaggagcc ccggtagggg
    22141 cggacccccg gagggcttca tgccccgcga cgccgtccac ggagtgaagc tcgccgccct
    22201 gggggagtac gagaccaagc tcacgacaac ccggaaggag ccccggtagg ggcggacccc
    22261 cggagggctt catgccccgt gatgccatct gccgagggaa gctcgccgcc ctgggggagt
    22321 acgagaccaa gctcacgaca acccggaagg agccccggta ggggcggacc cccggagggc
    22381 ttcatgcccc gtgatgccat ctgccgaggg aagctcgccg ccctggggga gtacgagacc
    22441 aagctcacga caacctggaa ggagccccgg taggggcgga ccccttcgcg ctttgcgaga
    22501 aggcccgccc cccccaaggg agattcgtca ggcggaggtg ccgcccgaag cgtcgcccct
    22561 ccctaccgcc gccacaagat taccaagggc agcgccagca ggagaatccc cgaaacgggc
    22621 cccgcgctgc accctccccc gccgcctccc ccgccccccg tgggagccac cgtgggattc
    22681 cctgtgggat tccctgtggg ggccgccgtg ggactccccg tgggaggtgt tgtgggggcc
    22741 accgtgggcc aatggatcgt cggcacgggg gctagcgtgg gttgctttgt gatgatggga
    22801 acgtacgagg gaataggcat gggcgtcacg gtggggaaga tcgagggaaa gggaagtttg
    22861 aacgagtcgt aggtctcctt cagctgaatg aactgcgacg actcgtagta cgtaccgggc
    22921 cgtccgtctt ttacttttat ccctttatcc tttaaaccgt cctctatctg ggccaccgac
    22981 aaatagggat aggccgcctt gagcaccgca aaggcccccg tcacgtgggg cgtcgccatg
    23041 gaagtgccat cgataggagc atagaggtcg tacgggatgg acgaccacac gctcacgccc
    23101 ggggccaaaa ggtccacgaa gtacgctatg ttggaaaagg gcgccaagtt catgtacttg
    23161 ttcgtggctc ccacagtgac cgccgtggaa atgcaccccg gagtccctgt ggcgttggag
    23221 tagccgtcgt tccccgagga gatcaccgtg gctatgccca cggaccgcag gttgtcgatg
    23281 atggccttgc gggcatcctt atcgcaaacc gaggcgtgtt tctccgaccc caggctcatg
    23341 ttcacggcgg cgattttata ggtgttcctg agggcataga cccgctccat gccccggatc
    23401 tggtccgagg cccacgacag ggcgtctccc ttcccctgac cgccgtagtt ggggaaccag
    23461 gagaaaacct gaatggcgat gatgtcagcg ctccgagcca ccccatgcat ggtgggaacg
    23521 ctgcccgccg cgatccccgc cacgtgggta ccgtgataaa accttgtccc gtaggtgccc
    23581 cccaagggag gcaaggcgga attggccgca gtggattcgg cggctccgcc ggggcagacg
    23641 gactgacagt cttgcccaga gtcggtggtg gtgtagcacg cctcggaaac caccttgccc
    23701 gccaggaagg ggtggctttt atctacccca ctgtccagaa tcgccacaca ccaaccgctt
    23761 cccccttggc cttcggacca gaccgccggg gcgtggatca gggggatggt gtcgttgagg
    23821 taggccttgg agagcccatc ctcttgcacc gagatcacgt cgggatcgac caaaagggcc
    23881 ttcagggcct ccgcgctcac ctgcatcgcc atgtagggaa tggtccgaaa cttcgtcacc
    23941 ggagaccccg cgagcttatt ccccagccgc gccagcaccc gcccctgggc cgtggcgatg
    24001 ccgttttgct gcacaaaggc cctccccggg ccaatatcgc cctcgggacg aaaatccgcc
    24061 cccaatccca cgatgacccg caccaccccc atctcggccg ccttggcctg cagagccgcc
    24121 acggccttta tggagcaaga gccctctgct tcccccgacc cccacaagac cgccagggcc
    24181 catatcccca gcacccaggt attcatcctc cctctcatac tcatctcctc ctccaccgaa
    24241 tcctgctctc tcccgaaggc ccgacccgaa gtagggccgc accggtcttc cccccggagc
    24301 cctctccatc gccgagagag gaaggtttcc ctcggcgccc cccaccggac aaccccatcg
    24361 aaggccgtct ctgacgatcc atgctatcta gatcatgagg aaaagtcaat atgtcttagg
    24421 gatccttgat ccgcaggttc atggcgagag tcgctgtata cagcaaaaaa aggcagctct
    24481 ggctttgcga agcctgctgg cgtcgtctct gtggggattg aggcggcgaa gtgggcaaaa
    24541 aaggctttca atgtccacgc gaaccaatct ccctccctgc gagaagaagg cagtttctgc
    24601 acatctttgg gcaaagccga atcaaccact catgcagtgt tttcacagaa ctgaggcaat
    24661 gctttgagcg cctcttccgc aatatgagcg aagtgtcctg ccggggtgag aattgtcttc
    24721 cgggcatgac gagaaatgcg ccccggaatg gcgtagagcg ttgccaaaag acgtttttgc
    24781 cgcacacgcc cccacccaga gggcagaaca tggaggagaa gaactttctg cagattcaac
    24841 gcgagcaccg tcaagtgcca ccaggccgcc gatgcgccaa aacttgccga gggcacgtgg
    24901 ccacccgcaa ggtcattttt gagaatggcg tgcatctctt ccgcccttcc gcaccgggct
    24961 cgctgccagc gaataacgtc ctctcccggc atgtcgtgca ggttcgtcac aatggcgaat
    25021 atcttccaga ttttaccgtc gagaacggtc aggtgcagct tcctcacccg aggatgggcc
    25081 tgctcaaggt cgtcgatatg ggccgccatt tcttcgaagg tgagctggca cacatccggt
    25141 ttctccgcat cagagagagc cgccgggcta cgcagtacaa ggaaacgata ct
//
LOCUS       DAOOER010000027        23886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_2452_length_23886_cov_8.737149, whole genome shotgun sequence.
ACCESSION   DAOOER010000027 DAOOER010000000
VERSION     DAOOER010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 23886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23886
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2452_length_23886_cov_8.737149"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(103..954)
                     /locus_tag="PL162_05215"
     CDS             complement(103..954)
                     /locus_tag="PL162_05215"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HQF91456.1"
                     /translation="MGYDEWYAPKAPRKARGGIKAASRRGDFAENWWGSGWIEALESL
                     PAGARWARGKNYARRGQVLSLEVRGGTVEARVQGTRVRPYRCCIDITPWGEDTWERVR
                     RVVGEHAYLAAALLGGRVPPELEDALRREGLSLFPFQEDRLAMACSCPDSSVPCKHLA
                     AVVYLLGERLDQDPFLIFELQGMPRREILRRVGVEGLAPEEGPSSLETLPGNDGEAFW
                     KGGPLPPIPETLGKGDPAALLRQLGPFPLWRGDEPLEEALAPVYRAVRDKWAQGAGGA
                     TPGTSPD"
     gene            complement(957..4028)
                     /locus_tag="PL162_05220"
     CDS             complement(957..4028)
                     /locus_tag="PL162_05220"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQF91457.1"
                     /translation="MIILHVLWRDDGLVLWGEAPRGTLAEEGPPGVTKPERGRALPPR
                     HPFEAGKALGEGWRHLLKGLGQEDAPKIPLREQVLMLPSLPRSPLPSSPLLGDFPPAR
                     GRAVVRLRPWKVTTLSQGVREALPWLLALAPDFPGFGGTVLGGDVACWAAAARFVRSL
                     VARGRFAPDLDLSCPGGPRSRWSPVLTGEDRGAWARLVRAFPPGIRAPERGERAPSAE
                     EVLRAFLAEAVDGLVRCALLPGRSREAPSADASWVAGLCRSDDPTLGGGREGRSDLAL
                     RLRKWRHPLEVMASSPWRLAFRLEEPEAGGSDPWNVRYLLQSLEDPSLLVPVTEAWEE
                     GFPFGRSVELREYVLTALGQAGRWCPPVEEGLRKGRLEGCDLAMPGVMAFLESQAPAL
                     EEAGFVVLTPGWWRRGRSRRRLGVRARVRGRSSVGAEALGIDDLVEVQWEVLLGSASM
                     TLKELEDLARLKRSLVRFRGEWVHLSPQELEASLELLRQGEERLSLREILHLELGATS
                     PPGDLDFEGVRGEGPLAKLLEGMAPEGSPDPLAEPENFAGSLRPYQRRGLGWIDFLAR
                     WGLGGCLADDMGLGKTVQTLAFIQRHKNAGGKAVLLVCPTSVMENWRREMGRFTPALK
                     ALVHHGLGRRKGESFHRHCAAADVVITSYALLGRDLSVLGDHPWGGVVLDEAQNIKNP
                     ETRAAQAARSLSAGFRLALTGTPVENDVGDLWSLFAFLNPGLLGSRGAFDRRYLGPIQ
                     RGQDPEASERLRRLTGPFVLRRLKTDRSLVEDLPDKVEREVFCPLTREQASLYRAVTE
                     ETAEALEKLQGPELRGVTLGAITRLKQICNHPAHFLKEDAPLEGRSGKLERLGEILEE
                     VRAVQDRVLIFTQFAEMGELLRRYVGERFGQEALFLHGGVPRLRRDAMVRRFQEDLQG
                     PSVFILSLKAGGTGLNLTRAHHVVLYDRWWNPAVEAQAVDRAFRIGQNRNVQVHRFLC
                     IGTLEEKIAALLERKREVASLSVASGEGWLASLSPEELRHLLALEKSALEG"
     gene            4550..5851
                     /locus_tag="PL162_05225"
     CDS             4550..5851
                     /locus_tag="PL162_05225"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQF91458.1"
                     /translation="MEAKAKHILLVEDQRVAALAQRAILFSLGYAVDIALSGPEALEK
                     HRHHRYDLVLMDIELGTGMDGGEAARQIQEEREIPVVFLSAHTDAPTIDRTRVTSHYG
                     YVVKGTGDALLQATLETALALFEARSRLRESEARYRLLIENTHDIIYALDPEGVFTYV
                     SPSWTKIVGHAPEDVVGTSYKKHVHPEDQEACNVFFKKFLDGASSLSGVEYRVLRQDG
                     RWAWHTSSATAARNGEGRMTSFIGISRDISERKVLEEALRHQATHDTLTGLPNRTLFL
                     DRLEHELAVTKRRGRGGALIFLDLDNFKNINDTLGHDVGDQLLVAVARRLKALVREAD
                     TVARMGGDEFTMLLPEAGGAPQVADVALRICQALHDVFLLDGQELFVSASMGISLYPH
                     DGTSVTTLLKLADMAMYRAKAAGRSTYRFWSGPDPTPPEAR"
     gene            6059..7933
                     /gene="pepF"
                     /locus_tag="PL162_05230"
     CDS             6059..7933
                     /gene="pepF"
                     /locus_tag="PL162_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HQF91459.1"
                     /translation="MEKKIGLAVACLWCFAAGEAGAALSGDVPLRSAQDPATQWRLED
                     IFPDGDAWEKERDAVEALLPRLAGFSGRLGTSGTELLEALRQEDEIGERLGRVYAWAH
                     MKSHEDTTDPGAQGRADRATLLSVKAASAGAFLAPEILALSPDVLEGFFRDVPALAPY
                     RFALDRLQRVRDHVLPADQELLLAQMGQVAETPETVFSLLTNADLKFPRIQDEEGREV
                     ELSEERYGRLIFSPHRAVRRDAFLGLLTTYGGMKNTLAATLAGSVKKDVFFARARRYS
                     SSLEASLFPGAIPEAVYTNAIKTVNDHLAPMHRYMTLKKKALSLDELHMYDLYVPLAP
                     ELRRDIPWEEAQRMVREALAPLGEPYVRELEKGFSAGWIDVYENKGKRHGAYSWGAYG
                     VHPYVLLNYNGTLRDVFTLAHEMGHAMHRFFTQATQPVTYAGHSIFTAEVASVTNEIL
                     LLEYLLRTTSDRESRLYLLNYALEQIRTTVYRQMLFAEFELETHRRAEAGEALTPDVL
                     SQIWRGLNERYYGPDVVVDPELEMEWGRIPHFYSAFYVYQYATGLAAAADLAQKILTE
                     GPPAVERYLTFLKSGDSAYAIDLLRTAGVDMAGPEPLEAVARTFSTRLDELQALLEGN
                     "
     gene            7938..8828
                     /locus_tag="PL162_05235"
     CDS             7938..8828
                     /locus_tag="PL162_05235"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HQF91460.1"
                     /translation="MTASVFRRPGAAALLWIRREWRDLVPITLGAIVVSVSYMAFLLP
                     NRFPASGLNGLAVLSTYVWDISPSWIVGLGNALLLVFAWKALSPSFVIKTIYAVGLMT
                     VLLKVFQGMPYPHLEERLLVALLAGILNGIGGGLVFRHGGCLGGTDVLVMVLRDRFGI
                     EVGTFSFLLSSSVFVVALPVVGLANVIYGGVVLYVTGLAIDGVLRSFDRRSQVILITA
                     IPTQVAEFVMKELHRGVTRIAGVGAYTGQERPILLCYLGPRQVVELRRFLAAHDPKSF
                     MALSEAAEVRGRGFKSWKGL"
     gene            complement(8943..12191)
                     /locus_tag="PL162_05240"
     CDS             complement(8943..12191)
                     /locus_tag="PL162_05240"
                     /inference="COORDINATES: protein motif:HMM:NF024904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQF91461.1"
                     /translation="MWISDFSLSRFGIFVDQRGTDLPPGLVVFAGKNESGKTTLMHFF
                     RYIFFGLPRRAGSNNLYAPIDGAPHRATLTFRTESDEDGASYTIEMRGRQVSLRETPS
                     GRTLGDLMGHIDQETFERIFCLGLEDLQGLKALQGRQIGSRLFAAGAGLGVVALPDLL
                     RELDQSRKSLYALGRSRGGKGTVNVLLEELRAGERSLQELEGVSSRYWSAVEDRESLE
                     EKLRRDEEELGLLVRRMAHLEMLEKARGAWGEYDLARQELATFHGPEDFPEKGGERMA
                     ALVAAVAELDAEIREGEALLKDLAVRRWQVDSVTPRGLLENAELISAVRGERGRFAAL
                     TADLPRRKQQATDREKDFQEALRQAGPDWTEERLFRVDLAFDTLQEAHSQEEELMSRQ
                     EELHRVELLADEGRKRLDAAEVLHMERSALLSRMEIPEVQDPERLQRQKEGLGRLRVF
                     FSQRAPIVMGKDLTREQMEAASEELRRKQDLRPRATATLPLWLALLCLGAGLGAAGGA
                     HYLGEQLLWFLAALAGAFSLILGFRAWRQMSRDEIQYRQWREDMEEKDRDVVRFGERI
                     HAFEEELKTLDRLVTELANDAGLLVPDTLEALEREVLGLEARGEQLKAFLDAKAACAV
                     ALRGRDEAESALKGLQEEVDDRRNEVRKAQDQWDSWLQAHGFGSGVRPGTFRELVARV
                     ERARGEFRALEEARRALEEAATYVRTQQDRVAHLLGLAGRGLPEGALPQPAHLEALLE
                     FFEGAQERQRDMADLDRQLEEIGRNRRLKEETLRQRKDERDRLLDQVGLRDETEFLAM
                     AQRCLRASELRELCLRRERELVLLVGHRGGIDALGEEIRQGSPAALAQEKEEGEGRLP
                     RLRQEIDEARRLLGVRDKEIADLRCDERQISLGEGISRLQARLRGAVREWLVTSICRF
                     CLEEARRRHERERQPEVIREADRSLQVMTGGRYALMATGSDNARTVELEERATGRRKG
                     EGAWSSGLGDQVYLALRFGLARLFGRKAEALPLILDDILVRFDEGRQEGAARVLLEEA
                     RHRQVLFFSCHEGTTRLLARMAQEEAFPPERRAFFRLDQGTIRSEDGLSEPL"
     gene            complement(12194..13468)
                     /locus_tag="PL162_05245"
     CDS             complement(12194..13468)
                     /locus_tag="PL162_05245"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HQF91462.1"
                     /translation="MAGDIVRFVHCGDLHLDAPFDRLEDIPGGLAPAIHGAPLRCWER
                     IVTLAIERGVDFVLLAGDLFDDVDRTLRAQLALVKGLERLSGAGIGAFIVCGNHDSLA
                     GWEARLPFPPGVYRFGGTAESLPFVRGGKTVATVTGYSYPQRRVEENVARLLRPSGEG
                     FQIALLHANVGHRREHGDYAPCALEDLVASGMDYWALGHIHAPGVLREASPAVVYPGI
                     PQGRRAHETGPRGCYVVTLGRGAPDLEFVETDLVRRERRGLDIASLGTEQELVRALEA
                     LRNDVRLDGGRGVLLRVELRGRGELHDTLIRPGIVTDLVLHLREGEELREDFVWTEAL
                     EDRTASWANREALSQEDHFVGDFLRSVEALEGAEDLVARVTALLDADPEGAGWNDRNV
                     VPLVEELLRQEAEEIFDQVKTRGLDGLLRQEG"
     gene            13677..14291
                     /locus_tag="PL162_05250"
     CDS             13677..14291
                     /locus_tag="PL162_05250"
                     /inference="COORDINATES: protein motif:HMM:NF022215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2585 family protein"
                     /protein_id="HQF91463.1"
                     /translation="MPLPEKTTLPPSSDPFEGPGAPGRLVGALGLLGGILALVLSLEG
                     RSLWGACSGFSLWEGDIWSACCSQRLLDPYSFTHGLHGLVLGGILAWLLPRASLSLRG
                     LGALSLEALWEVAENSPFIIQRYREATIALGYTGDSIINSLGDVLCCGAGFVLAHHLG
                     WKKAALVFMVTELAMLFWVRDNLTLNVLMLLCPIDAIKAWQMGL"
     gene            14872..15294
                     /locus_tag="PL162_05255"
     CDS             14872..15294
                     /locus_tag="PL162_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HQF91464.1"
                     /translation="MRYAATEEIIAIRLSEGEELHPALRHVCEEFNVDSAVVVGAVGM
                     VRQVTFGWFNGQEYLKTDFDGVFELLALNGNVSIKDQALYPHLHAALSRPDHTTVGGH
                     VLRAVADHNLEIFIKPLATVRLTRQFDGWFEAIVPVRR"
     gene            complement(15428..17542)
                     /gene="recG"
                     /locus_tag="PL162_05260"
     CDS             complement(15428..17542)
                     /gene="recG"
                     /locus_tag="PL162_05260"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HQF91465.1"
                     /translation="MPPKGAHPPGIAPPLPRGEDFGAPVRSLRGVGERLGALLARRGV
                     RTVRDLLFFFPRKYEDRRTIRSPGEMVPGERGAVLARVERLERVPTRREGLLLVRATL
                     VGDRGDRGSAVWFSRRGLERQIPPGTRALFYGRCEAGTGGGQLVHPDFDVLAPGEVPS
                     GGILPLYPGTAGLSQRRLRHLIQGLLAPGAPLPPDPLPEDLRRRLALVSLDQALREMH
                     FPRGNQEWRQARRRLVFEEFFLLQVAFSLRRRLRRAAAALPLAGDGAWQEDFFSRLPF
                     RLTRAQRRAWEELREDLRRPVPMHRLLQGDVGSGKTVVAAAALAAAAAAGSQGVLLAP
                     TEALARQHGDRLGQLLAPLGWEPLLVLGGGAEKERRRVREALASGEARVVVGTHALIA
                     KGVTFRHLALVVVDEQHRFGVLQRAALAGKGIEPHVLVMTATPIPRTLLLGSYGDLDV
                     SVLDEKPPGRKPVVTRWIPQDRRDEALEVLRRELDAGRQGYWICPLVEESENPSRSVA
                     AVTRRYRALQEVLAGHAVGLLHGRLPSREKARVLEDFATGRLALLVSTSVVEVGIDVP
                     GATVLVVEDAPSFGLAQLHQLRGRVGRGKDASVCLLVGDPSTPEGRGRLEALCASEDG
                     FFLAEEDLRLRGPGDLGGVRQHGLAYFRVADLGRDGALLEMARQEARRLSEEDPRLEG
                     HLPLLLRVEEEWEERFSLLGAG"
     gene            complement(17542..18096)
                     /locus_tag="PL162_05265"
     CDS             complement(17542..18096)
                     /locus_tag="PL162_05265"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HQF91466.1"
                     /translation="MTTLLTALEVANQEFRRELRGYHRGDVDDFLDRVAETLQQYAEL
                     VQEHERRWRASEDELREYATQKEALQRALLLAEQTSREIGEKAEREREALVAEAREQA
                     EKILAEAREEAQGAVVRLRQGEEDAARQLERLVCLRRQAVAETRELLSKLGMVLDAQE
                     KIARSSEAAWAVSPGACPDPAKPS"
     gene            complement(18093..18488)
                     /locus_tag="PL162_05270"
     CDS             complement(18093..18488)
                     /locus_tag="PL162_05270"
                     /inference="COORDINATES: protein motif:HMM:NF016363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HQF91467.1"
                     /translation="MFEKLLARLGILEEVEEEFEEDDEQWKERKRRVLEPAPPEAVLV
                     VVRGLQCVHRREEVAEALHQGKLVLVDLRLVERALGQSALDFICGVAYGTKGAVMRVV
                     PGVFLAMSHRALVEEWSDKALATDGELTI"
     gene            complement(18505..19290)
                     /locus_tag="PL162_05275"
     CDS             complement(18505..19290)
                     /locus_tag="PL162_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HQF91468.1"
                     /translation="MVAYGGSEREGLLSAATPLGERVARVRARVEEAARSCGRDPREV
                     RLCAVSKYHPLEAMRQAAEAGVDLLGENRVQEAGEKARAWPPNAPRVPWHLLGHLQRN
                     KARRALEIFDAIQSLDGEPLALTLQRLCEERGRGVTVLLEVNISEESAKTGADPRAAA
                     DLLEAVQRRCPLLAVEGIMAMAPLTDRVEERRRAFAALRELRDRLAKGSGLSLGVLSM
                     GMSDDYPEAIQEGSTLVRVGSAIFGPPEGRGGDDGDLLARPKE"
     gene            complement(19284..20273)
                     /locus_tag="PL162_05280"
     CDS             complement(19284..20273)
                     /locus_tag="PL162_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91469.1"
                     /translation="MGRFFLKKLLPRGSRGKAPEAPPRRLSWKMRAYLAMVVFMVSGG
                     VAVYVSWFRHYQESHPRVTWGRPERYAEEHLAELALVWREEVIPSPATGTLSLAGKGR
                     ICRVARGDALATVKGGGGAVVVRSPAPGYFVPYLDGQEGKWGFSSLWRGDGVLPDPPS
                     PKAIAEGSPVTRGQGVGKLVYQPQELRCIAYLPLTPAVAEEIRRGRVKVRLASGDRPV
                     MMELRAHQTYGVREKIYGTLPVFPPGMLPGRRLAVILLGREQEGTAIPEEAVTFTGGR
                     QVVFVVTGSELRLRDVQGIPLPGGRFLVTEGLAAGELVLEKAEGAREGRVKLW"
     gene            complement(20473..21108)
                     /locus_tag="PL162_05285"
     CDS             complement(20473..21108)
                     /locus_tag="PL162_05285"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQF91470.1"
                     /translation="MKAQVVLFDFDMTLADSVEAITYCFNRVAEDKGLPAVTSQEVGA
                     TIGLPFTEACMRLWGRGDPELLAWYGDHYREDEFSRMKLFPDAPGVLTRLRHEGMRTG
                     VVTNRRYARRVVERVGILPLCDVVVGLEDGVRGKPEPDLLLRALEILSRSPEGAWYVG
                     DTDGDMRAARAAGMGALGIAQGSFGPRELEEAGAHGVVSSLSAVLTYLGLS"
     gene            complement(21157..22086)
                     /locus_tag="PL162_05290"
     CDS             complement(21157..22086)
                     /locus_tag="PL162_05290"
                     /inference="COORDINATES: protein motif:HMM:NF014017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HQF91471.1"
                     /translation="MGEIRVGVCSWDDRGLRGSGWYPAPLRNAGARLGYYAERFDTVE
                     VDSVFYALPREEWIFRWVSRTPPGFLFNIKAFGLFTWHRVPVRALPLWAREGLAPGAS
                     QVTLDDLPRETRLSLWERFVQALAPLHRMGRMGYLLFQFPPWVRPGGRLEAYVRRLAS
                     LARPLRLAVEVRHRSWWDDPHRGRLLDLLRRENVAYVAVDEPDLPWTMPPEWPRTASW
                     GTVARFHGRAAEAWSRPGASVEERFRYLYAPEELRPWRDRVWAQASQDGKVFVMFNNC
                     GGEGAVRGAALLKGMLGLAPAEEGGEQRRLPGL"
     gene            22408..>23886
                     /locus_tag="PL162_05295"
     CDS             22408..>23886
                     /locus_tag="PL162_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HQF91472.1"
                     /translation="MNNGKSEKTERPAEIFNKHVFSQAAMAERLPREVFDRLRGVVEG
                     REKLDIHVAGIVAMAMKEWAVGLGATHYTHWFQPRTELTAEKHMAFLNVNDAGLPLEF
                     FSGGELIQSEPDASSLPSGGMRSVFEARGYTGWDPSSPAFVVSHSRGGTLCIPSVFCS
                     YDGTPLDMKTPLLKAIGALEERALRMIRLFGNRGVRWVHATVGGEQEFFLVDEALASR
                     RPDLVFCGRTVMGCPPPKGQRMGDHYFGAIHPRVLEFMEDAQDALARLGVAIRTRHNE
                     SAPGQFEFAPHFAEANLAADQNQITMETLRTVARRHGFLLLLHEKPFAGLNGSGKHIN
                     FSLQDSEGRNLLKPAGGNERRGLQFLAFLTALLRGMRDHGGLLRASVAGPGNVHRLGG
                     HEAPPPIMSVFLGSVLTRLLEDLEEGVQSAIDPRGDLDLGLFRLPALRTENTDRNRTA
                     PVAFTGNKFEFRAPGASQSMSGPLTVIFALWARGMDVVMAAIQ"
BASE COUNT         4000 a         8760 c         7322 g         3804 t
ORIGIN      
        1 tttctttggc ccaaaagacg tcaaaaaaat agacattgct actgtctgtt gacagggagg
       61 atccccttag actttggcct cttttgtctc acgggataaa ggttaatcag gagacgtccc
      121 gggagtcgct cccccggccc cctgagccca tttatcccgg acggcgcgat agaccggcgc
      181 cagggcctct tccaggggct catccccccg ccataggggg aaaggtccca gttggcggag
      241 cagggcggcg gggtccccct ttcccagggt ctccgggatg ggaggaaggg ggccgccctt
      301 ccagaaggct tctccgtcgt tcccgggcaa ggtctccaga gaagaaggcc cttcctccgg
      361 ggcgaggcct tccactccca cccggcgcaa gatctccctc cggggcatcc cctggagctc
      421 gaagatgagg aagggatcct gatccaatcg ctcccccagg aggtatacca cggcggccag
      481 gtgcttgcag ggcacggagg aatccgggca ggagcaagcc atggccaggc ggtcttcctg
      541 gaaggggaag agggaaaggc cctcccgccg caaggcgtct tccagctccg gagggacgcg
      601 tcccccgagg agggccgccg ccagataggc gtgctccccc accaccctcc tcaccctttc
      661 ccaggtgtcc tctccccagg gggtgatgtc gatacaacat cgataggggc gaactcgggt
      721 cccttggacc cgggcctcca ccgtcccccc tcgcacttcc agggaaagga cttgaccccg
      781 ccgggcatag ttcttccccc gcgcccatcg ggccccggcg ggaaggcttt ccagcgcctc
      841 aatccaacca cttccccacc aattttccgc aaagtccccc cgacgcgagg cggctttgat
      901 ccctccccgg gccttccggg gagccttggg ggcataccat tcatcgtatc ccattcctac
      961 ccctccaggg cgcttttttc cagagccagg agatggcgca gctcctccgg tgacagggag
     1021 gccaaccatc cctcccccga cgccacggaa agggacgcca cctctctctt gcgctccagc
     1081 agcgcggcaa tcttttcttc cagggtcccg atgcagagaa agcggtgaac ctggacgttg
     1141 cgattttggc cgatgcgaaa ggcccggtct acggcctggg cttctacggc ggggttccac
     1201 cagcggtcgt aaagcaccac gtggtgggcc cgggtgaggt tgagccccgt gccccccgcc
     1261 ttgagggaca ggatgaagac cgaaggcccc tggagatctt cctggaagcg cctcaccatg
     1321 gcgtcccggc ggagccgggg gactccgccg tggaggaaga gggcctcctg gccgaagcgc
     1381 tcccccacgt acctccggag cagctccccc atctcggcga attgggtgaa gatcaggacc
     1441 cgatcctgga cggcccggac ctcctccaag atctccccca ggcgctccag cttgccggat
     1501 cgtccctcca agggggcgtc ttccttcagg aagtgcgccg ggtggttgca gatctgcttg
     1561 agccgcgtga tggcccccag ggttaccccc cggagctccg ggccctggag tttttccaag
     1621 gcctcggcgg tttcttccgt cacggcgcgg tagagggagg cctgctcccg agtgaggggg
     1681 cagaagactt cgcgctccac cttgtccggc aggtcctcca ccagcgatcg gtcggtcttg
     1741 agcctccgga gcacgaaggg gcccgtcagg cgccgcaagc gctccgacgc ctcggggtcc
     1801 tggccccgtt ggatgggccc caggtatcgt cggtcgaagg cccctcggct ccctagaaga
     1861 cccgggttca aaaaggcaaa aagggaccaa agatccccca cgtcgttttc cacaggagtg
     1921 cccgtgaggg ccaaccggaa gcccgccgac aggctccgcg ccgcctgggc cgcccgggtc
     1981 tcggggtttt tgatattttg ggcctcgtcc aggaccactc ccccccaggg atggtccccc
     2041 agcaccgata gatcccgccc caaaagggcg tagctcgtga tcaccacgtc ggcggcggcg
     2101 caatggcggt ggaaggactc cccctttctc cggccgaggc cgtgatggac caaagctttc
     2161 agggcggggg tgaaacgccc catctctcgg cgccagtttt ccatgaccga ggtgggacac
     2221 accaggagca cggccttgcc cccggcgttt ttgtgtctct ggatgaaggc caaggtctgg
     2281 acggtcttcc ctaaccccat gtcgtccgcc agacaccccc ccagccccca ccgggcgaga
     2341 aagtcgatcc accctaagcc ccggcgctgg tagggtcgca gagatccggc aaagttctcc
     2401 ggttccgcca ggggatccgg agatccttcc ggggccatcc cctcgaggag cttcgccagg
     2461 ggaccctccc ctcggacgcc ctcgaaatcg agatcgccgg ggggagaggt cgccccaagc
     2521 tccaggtgaa ggatctcccg gagggaaagc ctttcctctc cctgacggag gagctccaaa
     2581 gaggcctcga gctcttgggg agacaggtgc acccattcgc cgcgaaatcg caccagggac
     2641 cgtttgaggc gcgccagatc ttccagctct ttcagagtca tggaagccga tcccaagagg
     2701 acttcccatt gcacctccac caggtcgtcg atccccaggg cctccgcccc caccgagctt
     2761 cgccccctca cccgggcccg cacgccgagg cgcctccggg agcgcccccg gcgccaccac
     2821 cccggcgtca gcacgacaaa acccgcctcc tccagagcgg gggcctggct ttcaagaaag
     2881 gccatcaccc caggcatcgc caggtcacac ccctccagac gcccctttcg caatccctct
     2941 tccacggggg ggcaccatcg ccccgcctgc cccagggccg tgaggacgta ttcccgcaac
     3001 tccaccgacc ttccgaaggg gaatccctct tcccacgcct cggtcaccgg cacgaggagg
     3061 ctggggtcct ccaaagactg caacagatat cgcacgttcc aaggatccga gccgcccgct
     3121 tcgggctctt ccagccgaaa ggccagacgc cagggggaag aggccatcac ctccagggga
     3181 tggcgccact ttcgcagcct gagggccaga tcgctccttc cctctcgtcc tcctcccagg
     3241 gtggggtcgt cgctgcggca gaggcccgcc acccacgagg cgtccgccga tggggcctct
     3301 cgggatctcc ccgggagaag ggcgcatcgc accaggccgt ccaccgcctc cgccaggaag
     3361 gcccgaagga cctcttccgc cgaaggagcc ctttcccccc tttcgggggc ccggatcccc
     3421 gggggaaagg cccgaaccaa gcgggcccac gcccctcgat cctccccggt gaggacggga
     3481 gaccatcggg atcggggacc cccgggacac gacagatcca gatcgggggc gaagcgcccc
     3541 cgcgccacca gggaccggac gaaccgggcg gcggcggccc agcacgccac gtcgcccccc
     3601 agcaccgtcc ccccgaaccc gggaaagtcc ggagcgaggg ccaaaagcca gggaagggcc
     3661 tctcgaaccc cctggctcag cgtggtcacc ttccaagggc gcaggcgcac caccgccctt
     3721 ccccgggctg gagggaagtc ccccaggagg ggggatgagg gaagggggct ccggggaagg
     3781 gagggcaaca tcaagacctg ctccctcaag gggatttttg gcgcatcctc ctgtcccagc
     3841 cccttgagga ggtggcgcca cccctccccc agggcctttc cggcttcgaa gggatgacgg
     3901 gggggcaaag ccctccccct ctcgggtttc gtcacccccg ggggaccttc ttccgccaag
     3961 gtcccccggg gagcctctcc ccaaagcacc aggccgtcat cgcgccaaag gacatgcaaa
     4021 attatcataa aaagctttcc accctctcca aaatcttcgc gaactctttc cccaccatcg
     4081 cctgagggct ccctcgcgac cgccgcccat ccccggagta tacctttttt ccgcgatgag
     4141 gtctcgcccg gaaggcggcg aagggggcgc cctccggccg gcccctagcc cggagggctc
     4201 gggtcccggg gagctcgctc cggcgccctt gagccccaga ccagaagaag aaggcgcaaa
     4261 aacccctggc gccgcgtggg cagtgcgatg gaagcttggg ggagctcccc gatctcacgc
     4321 cggagaagta cggtcctcat gggggcaaac cggagaaagg gcccctacga aatacggcgg
     4381 aaccggcggg cccacctggc ccgtcggagc ccccctgtct tctttgggca aaaaggtcta
     4441 gaatagggag tcctcccatc tctccggccc ccggggtcat cctctcaaag gggccttctc
     4501 cgaaagggga gcccattagg gcgatgattg gaaaaaaggg gtgtttttct tggaagcgaa
     4561 agcaaagcac atcttgctgg tggaagacca acgggtagcg gccctggccc agcgggccat
     4621 ccttttttcc ctgggatacg cggtggacat cgccctttcc ggccccgagg ccctggaaaa
     4681 acaccgccat caccgctatg acctggtgct catggacatc gaactgggca ccggcatgga
     4741 cgggggcgaa gcggcccgcc agatccagga agagcgggag atccccgtgg tctttctttc
     4801 ggcccacacc gacgccccca ccatcgaccg cacccgagtg acctcccact acggttacgt
     4861 ggtcaagggc acgggagacg ctctgctcca ggccaccctg gagacggccc tggctctctt
     4921 cgaggcccgg agccgcctcc gggagagcga ggcccgctac cgcctcctca tcgaaaacac
     4981 ccacgacatc atctacgccc tggatccgga gggcgtcttt acctacgtct cccccagctg
     5041 gaccaagatc gtggggcatg ctccggaaga tgtggtgggc acgagttaca aaaagcacgt
     5101 ccaccccgag gaccaggagg cctgcaacgt gttcttcaaa aaattcctgg acggcgcctc
     5161 ctccctttcg ggggtggagt accgcgtgct ccggcaggac gggcgctggg cctggcacac
     5221 ctcgagcgct acggcggccc ggaacgggga agggcgcatg acctctttca tcggtatatc
     5281 ccgggacatc tccgagcgca aggtgttgga agaggccctg cgccaccagg ccactcacga
     5341 caccctcacg gggcttccca accgcaccct cttcctcgac cgcctggaac acgaactcgc
     5401 ggtgaccaag cgccggggcc gggggggagc gctgatcttt ctcgacctgg acaacttcaa
     5461 aaacatcaac gacaccctgg ggcacgacgt cggagatcag cttctggtgg cggtggcccg
     5521 ccgcctcaaa gccctggtcc gcgaggcgga caccgtggcc cgcatggggg gagacgaatt
     5581 caccatgctt ttgcccgaag ccgggggggc gccccaggtg gccgacgtgg cccttcgcat
     5641 ctgtcaggcc ctccacgatg tttttctgct ggacggccag gagctcttcg tttcggccag
     5701 catgggcatc agcctgtacc cccacgacgg cacctccgtc accaccctgc tcaagctcgc
     5761 cgacatggcc atgtaccggg ccaaggccgc cggacgcagc acttaccgtt tctggagcgg
     5821 gccggatccc acgccccccg aggcgagatg agccccgcac gatagcgttg ggtccatccc
     5881 ctgatctcgg gcgccgaagc cccagggatc gaagaaatgt cccttccgca acgacgtcaa
     5941 tggggttgcc gccttcgttt ccccgggaca tcagaggatc gccccttccc cgaagccctt
     6001 tccggggaag tttaataaac ctaagagtcg gcatcgccga cggaaaggaa cggagatcat
     6061 ggagaaaaaa ataggccttg ccgtggcctg cctatggtgt ttcgccgcag gagaggcggg
     6121 ggcggcgctg tccggcgacg tccccctccg gagcgcccag gaccccgcca cccagtggcg
     6181 attggaagat atcttccccg acggcgacgc ctgggagaag gaacgggacg ccgtggaggc
     6241 cctgctgccc cgactggcgg gtttttccgg aaggctcggg acctccggca ccgagctctt
     6301 ggaagcccta cgccaggagg atgaaatcgg agagcgcctg ggacgggtct acgcctgggc
     6361 ccacatgaaa agtcatgagg acacgacgga ccccggggcc cagggacgcg ccgaccgagc
     6421 cacccttctt tccgtgaagg ccgcgagcgc cggagccttc ctcgcgccgg agatcctggc
     6481 cctctccccc gacgtgctgg agggattctt tcgggacgtc cccgccctgg ccccctaccg
     6541 cttcgccctg gatcgcctcc agcgggtgcg ggatcacgtg ctgccggcgg accaggagct
     6601 cctgctggcc cagatgggtc aggtggcaga aacgccggag acggtctttt ccctgctgac
     6661 caacgccgac ctgaagttcc cccgcatcca ggacgaggag gggcgggagg tggagctgtc
     6721 ggaggagcgc tacggccgtc tcatcttctc cccgcaccgg gccgtgcgcc gcgacgcctt
     6781 tctggggctc ctcaccacct acgggggaat gaaaaacacc ctggccgcca ccttggccgg
     6841 gagcgtcaag aaagacgtct tcttcgcccg ggcccggcgc tactcctcgt ctttggaggc
     6901 ctcgctcttt cccggagcca tccccgaggc ggtctacacc aacgccatca agacggtgaa
     6961 cgatcacttg gcccccatgc accgctacat gaccctgaag aaaaaggccc tatcgctgga
     7021 cgagctccac atgtacgacc tctacgtccc cttggccccc gagctccgcc gggacatccc
     7081 ctgggaggag gcccagcgca tggtgcgtga ggccctcgct cccctggggg agccctacgt
     7141 ccgggagctg gaaaagggct tttctgcggg gtggatcgac gtatacgaaa acaaagggaa
     7201 gcgtcacggg gcctactcct ggggggccta cggggtgcac ccctacgtgc tcctgaacta
     7261 taacggcacc ctacgggatg tctttaccct ggcccacgag atgggacacg ccatgcaccg
     7321 cttcttcacc caggccaccc aacccgtgac ctatgcgggg cactccatct tcaccgccga
     7381 agtggcatcg gtgaccaacg agatcctcct cctggagtac ttgcttcgca ccacctcgga
     7441 ccgggaatcc cggctgtacc tcctgaacta cgccctggaa cagattcgca ccacggtcta
     7501 tcgccagatg ctcttcgcgg agttcgaact ggagacccac cgccgggccg aggcggggga
     7561 ggcgttgacc cccgatgtct tgagccagat ctggcggggg ctcaacgagc gatactacgg
     7621 ccccgacgtg gtggtggacc cggagctgga gatggagtgg gggcgcatcc cccacttcta
     7681 cagcgccttc tacgtgtacc agtatgccac ggggcttgcc gccgcagcgg atctggccca
     7741 aaagatcctg acggagggcc ccccggccgt ggagcgctac ctgaccttcc tgaaaagcgg
     7801 ggactcggcc tacgccatcg accttctccg gaccgccgga gtggacatgg cagggcccga
     7861 gcccctggag gcggtggccc ggaccttctc cacccggctg gacgagctcc aggccctgct
     7921 ggaggggaac taggggcatg acggcctccg tgttccgacg ccccggggct gcagccctgc
     7981 tctggataag gcgggaatgg cgcgacctgg tgcccatcac cctgggggcg atcgtcgtct
     8041 ccgtgagcta catggccttt ctcctgccca accgcttccc cgcctcgggc ctcaacggcc
     8101 tggcggtgct ctccacctac gtctgggata tttctccctc ctggatcgtg gggctgggga
     8161 acgccctgct tctggtcttc gcctggaagg ccctctctcc cagcttcgtg ataaagacca
     8221 tctacgccgt gggactcatg acggtgctcc tcaaggtctt ccagggcatg ccctatcccc
     8281 acctggaaga acggctcctg gtggccctcc tggcggggat cttgaacggc atcggcggag
     8341 gcctggtgtt ccgccacggc ggatgtctgg ggggcacgga cgtcctcgtc atggtgcttc
     8401 gggatcgttt cggcatcgaa gtggggacct tcagctttct cctgagctct tcggtgttcg
     8461 tggtggccct acccgtggtg gggctcgcca acgtcatcta cgggggcgtg gtgctctacg
     8521 tcacggggtt ggccatcgac ggagtgctgc ggtcctttga ccggcggagc caggtaatcc
     8581 tcatcaccgc cattcccacc caggtggcgg agttcgtcat gaaggagctg caccgcgggg
     8641 tcacgaggat cgccggagtg ggggcctata cggggcaaga gcgccccatc ctcctgtgct
     8701 acctggggcc tcgccaggtg gtggagctgc gccgcttcct cgcggcgcac gaccccaagt
     8761 ccttcatggc cctttccgag gccgccgagg tccggggaag gggcttcaaa tcctggaagg
     8821 ggctatagcc ccggaagggt ctgggcaaag cccccccgcc taaggggcgc cccttttgca
     8881 agaagggggc cccccggatc cgaggggccc cccggatccg aggggcccct ggatcattgg
     8941 cactatagcg gttccgataa tccatcttcg gagcgaatcg tcccctggtc caggcggaaa
     9001 aaggcccgcc gttcgggggg gaaggcctct tcctgcgcca tccgggccag caggcgcgtg
     9061 gtgccttcat ggcaggagaa aaagagcacc tggcgatgcc gggcctcttc caaaagcacc
     9121 cgggcggccc cctcctggcg cccctcgtcg aagcgcacca ggatatcgtc caggatcagg
     9181 ggcagggctt cggccttgcg gccaaagagt cgggccagac cgaatcgcag ggccaggtat
     9241 acctggtctc ccaggccgct gctccaggcc ccctcgccct ttctccggcc cgtggcccgc
     9301 tcttccagct ccacagtgcg ggcgttatcc gatcccgtgg ccatcagggc atagcgcccc
     9361 ccggtcatga cctggaggga tcggtccgcc tctcggatca cctcgggctg acgctcccgc
     9421 tcgtggcgcc gccgggcctc ttccaggcag aaacgacaga tcgacgtcac caaccactcc
     9481 ctcacggcgc cccgcaagcg ggcctggagg cgggaaatcc cctcccccag ggagatctgg
     9541 cgctcgtcgc accgcagatc ggcgatttct ttatcccgta ccccgaggag gcgtcgggcc
     9601 tcgtcgattt cctgtcgaag gcggggaagg cgcccctccc cttcttcctt ctcctgggcc
     9661 agggccgccg gggacccttg ccggatctcc tcgcccagcg cgtcgatccc cccccggtgt
     9721 cccaccagta ggaccagctc ccgctcccgg cgaaggcaga gctcccgcag ctccgaggct
     9781 cgaaggcagc gctgcgccat ggcgaggaac tccgtctcat ccctgagccc cacctggtcg
     9841 aggaggcgat cccgctcgtc ctttctctgc ctcagggtct cttccttcag ccgtcgattt
     9901 cgcccgattt cctccagctg gcggtccagg tcggccatat ccctctgacg ctcctgggct
     9961 ccctcgaaga actcgagcaa cgcctccagg tgggccggtt ggggtagggc cccctcgggg
    10021 agtccccttc ccgccaggcc caacaggtgg gccacgcgat cctgctgggt gcggacgtag
    10081 gtggcggcct cctccagggc ccggcgggct tcctccaggg cccggaactc cccccgggct
    10141 ctctccaccc gggccaccag ctcccggaag gtgccggggc gcaccccgga gccgaatccg
    10201 tgggcctgga gccaggagtc ccactgatcc tgggcctttc gcacctcgtt ccgccgatcg
    10261 tccacttctt cctgcaagcc cttgagggcg gattccgcct cgtctcggcc ccgcagggcg
    10321 acggcgcagg ccgccttggc gtccagaaag gccttgagct gctccccccg ggcctcgagg
    10381 cctaacacct ctcgttccag ggcttctagg gtgtcgggga ccaggagacc cgcatcgttc
    10441 gccagctcgg tcacgaggcg atccagggtc ttcagctcct cttcgaaggc gtggatgcgc
    10501 tctccgaatc gcacgacatc ccggtccttt tcttccatgt cctcccgcca ctggcggtac
    10561 tggatctcgt cccgggacat ctggcgccag gcccggaagc ccaagatcag ggaaaaggcc
    10621 cccgccaggg ccgcgaggaa ccacaggagc tgttccccaa ggtaatgggc cccccccgcc
    10681 gcccccaggc ccgcccccag gcacaaaagg gcaagccaca ggggaagggt ggccgtcgcc
    10741 ctggggcgga ggtcttgctt gcggcgcagc tcctcggagg ccgcttccat ctgctcccgg
    10801 gtgaggtctt tgcccatgac gatgggagcc cgctgggaga aaaacacccg caggcgcccc
    10861 agaccctcct tctgacgctg gagtctctcg gggtcctgca cctcggggat ctccatgcga
    10921 gagagtaggg cgctgcgctc catgtggagc acctccgccg catccaggcg ctttcggcct
    10981 tcgtccgcga ggagctccac ccggtgtagc tcctcctgcc ggctcatgag ctcttcttcc
    11041 tggctatggg cctcctggag cgtgtcgaag gccagatcca cccggaagag gcgctcttcg
    11101 gtccagtcgg gccccgcctg ccgcagggcc tcctggaagt ccttctcccg atccgtagcc
    11161 tgctgcttcc gtcgcggcag atcggccgtc agggcggcaa aacgcccccg ctctcctcgc
    11221 acggcggaga tcagttcggc gttctccagc aggccccggg gagtgacgct atccacctgc
    11281 caccggcgca ccgccaggtc cttcagcagg gcctctccct cccggatttc cgcatccagc
    11341 tccgccacgg cggccaccag ggccgccatg cgctcgcctc ccttctcggg gaagtcctcg
    11401 gggccgtgga aggtcgcaag ctcctgccgg gccagatcgt actcccccca ggccccccga
    11461 gccttttcca gcatctccag gtgggccatc ctccggacca ggagtcccaa ctcctcctcg
    11521 tcgcgccgga gtttttcctc cagggactcc cggtcttcca ccgcgctcca atagcgggac
    11581 gagaccccct ccagctcttg aaggctccgc tctccggccc tcagctcctc cagcaggacg
    11641 ttcaccgtcc ccttgccccc ccggctgcgc cccagggcat agagggactt gcgggattgg
    11701 tccagttccc tcagcaaatc cggcagggcc accacgccga ggcccgctcc tgcggcgaag
    11761 aggcggcttc ctatctggcg cccctgaagg gccttgagcc cctggaggtc ctccagcccc
    11821 agacagaaga tcctctcgaa ggtctcctgg tcgatgtgtc ccatcaggtc tcccagggtg
    11881 cgccccgaag gggtttcccg caaggagacc tggcgccccc gcatttctat ggtatacgag
    11941 gccccgtcct cgtccgattc ggtgcggaaa gttaaagtgg cccggtgagg ggcgccatcg
    12001 atgggggcgt agagattgtt gctccccgcc ctgcggggca agccgaagaa gatgtaccgg
    12061 aaaaagtgca tgagggtggt cttgccgctc tcgttcttgc ccgcaaagac cacgagcccc
    12121 ggggggaggt cggtcccccg ctggtccacg aagatgccga aacgcgagag ggagaaatcg
    12181 gagatccaca tggctaaccc tcctggcgca agaggccgtc gagcccccgg gtcttcacct
    12241 ggtcaaagat ctcttccgcc tcttgccgga gcagctcctc caccaggggc accacgttcc
    12301 ggtcgttcca tcccgcccct tccgggtcgg cgtccaacag agccgtcacc cgggccacca
    12361 gatcctccgc gccctccaag gcctccacgc tccggagaaa gtcccccacg aagtgatcct
    12421 cttggctcaa ggcctcgcgg tttgcccagg acgcggtgcg atcctccagg gcctcggtcc
    12481 agacaaagtc ctcccggagt tcctccccct ctcggaggtg gagcaccagg tccgtcacga
    12541 tccccggacg gatcagcgtg tcgtggagct ctccccttcc ccggagctcc acccgaagca
    12601 ggaccccgcg ccccccgtcg agacgaacgt cgtttcgcag ggcttccagg gcccgcacca
    12661 gctcctgctc cgtgcccagg gaagcgatgt ccagccctcg gcgctcccgg cggacgaggt
    12721 cggtctccac gaactccaga tccggagccc cccttcccaa agtcaccacg tagcaccccc
    12781 ggggccccgt ctcatgggcc cgacgcccct gggggatgcc cggatagacc acggcggggg
    12841 aggcctctcg gaggaccccc ggggcatgga tgtgtcccag ggcccagtag tccatgcccg
    12901 aggcgacgag gtcctccagg gcacaggggg cgtagtcccc gtgctcccgc cgatggccca
    12961 cgttggcatg cagcagggcg atctgaaacc cctcccccga gggacgcagg aggcgagcca
    13021 cgttctcctc cacccgccgc tgggggtagc tgtatcccgt gacggtggcc accgtctttc
    13081 ccccccggac gaaggggagg ctctctgcgg ttccgccgaa gcggtacacc cccggaggaa
    13141 agggcagacg ggcctcccac cccgccaggg agtcgtggtt gccgcagacg atgaaggccc
    13201 cgatgcccgc gccgctcagt cgctccaacc ccttcaccag ggcgagctgg gcccggagag
    13261 tccggtcgac atcgtcgaag aggtctccgg ccagcagcac gaaatccacc cccctctcga
    13321 tcgccagcgt cacgatcctc tcccaacagc gcaggggggc tccgtgaatc gccggagcca
    13381 gcccccctgg gatgtcctcc agacggtcga agggggcgtc caggtgcagg tcgccgcagt
    13441 ggacaaaacg cacgatgtct cccgccatac cactttcctc cctctcggac ccctcgggcc
    13501 ccgatccgac gatgactccc gtcccggagg tattgtaccg ccatcggagc gaccagggaa
    13561 agggccatac cccgggggga tcgccccttc gtgccctcgg cgccctatgc tatagtagtc
    13621 ccggagggtt cccctctttt ctacaaaacc acctagaacg cgaggagcga tgctccgtgc
    13681 cccttccgga aaaaaccacc ctccccccct cctccgaccc cttcgaaggc cccggagccc
    13741 cggggcgcct cgtgggagcc ctggggctcc tggggggaat cttggccctg gtcctctctc
    13801 tggaggggcg ctccctttgg ggggcctgct ccggcttttc cctttgggag ggcgacatct
    13861 ggagcgcctg ttgctcccag cgcctcctgg acccttatag tttcacccat ggactgcacg
    13921 gcctggtcct cggcggaatc ctggcgtggc tccttccccg ggcttccctg tccctccgag
    13981 gactgggggc cctatccctg gaggccctct gggaagtggc ggagaactcc cccttcatca
    14041 tccagcgata ccgcgaggcc accatcgccc tgggatacac gggagattcc atcatcaact
    14101 ccctggggga cgtcctctgt tgcggcgcgg gcttcgtcct cgcccaccac ctggggtgga
    14161 agaaggcggc cctggtcttc atggtcaccg aattggcgat gctattctgg gtgagggaca
    14221 acttgacgct caacgtcctc atgcttctat gccccatcga cgccataaag gcctggcaga
    14281 tgggactttg atccccctca tccccgagtc ccccgccgga ctcgggatgg gaggagggaa
    14341 aaaagcaaag ggacgagaga aaacggtgaa gcgatcgccc cttcgacgag gccccgacga
    14401 cgacacgggg cgaggaagag ttctccctcg tcgtctccgc ccttcccccc gaccccgtcc
    14461 gatcaggggg agtggtggcg ggtgccaggg gggaggggcc cccctggcgt cgctcctggg
    14521 gctccccctg atcctcgtga tcctgaaaag atctcgaata atctaaaggc cccgaggccg
    14581 ggcgagatga agggggtgtt tcccgggcca ccgaagaggc gcgcccggaa gggcctcctc
    14641 cgaactcccc tggggaaggg gccgtgccgc ctgaacaatt tggatttctc cttggggtgg
    14701 cgggggccac ggtgtggtct tttttcgtgt tcctggcggg taggctttcc gaggatcctc
    14761 gctaaaagag gctcttcccc cggggacttt ttttctttct ttcgcttttg gagttgtgcg
    14821 ttgatggaag cgtgctacaa tgggatcaat cctacgctag gaggtgtttc catgcgatat
    14881 gcggccacgg aagagatcat cgccatccgg ctttccgaag gggaggagct ccaccccgcc
    14941 ctgaggcatg tgtgcgagga attcaatgtg gattccgctg tggtggtggg agcggtgggc
    15001 atggtacgac aggtgacttt cggatggttc aatggtcagg agtacctgaa gacggatttc
    15061 gacggggtct tcgagctcct ggccctgaac ggcaacgtca gcatcaaaga ccaagcgctc
    15121 tacccccacc ttcacgccgc cctgagccgc ccggaccaca ccaccgtggg ggggcacgtg
    15181 ctccgcgccg tggcggacca caacctggag atcttcatca agcccctggc caccgtccgg
    15241 ctcacccggc agtttgacgg ctggttcgaa gccatcgtcc ccgtaaggcg ataggacccc
    15301 ggacccccac cggtgggacc acccctccgg tggggggttt tttatgcccc cgggggcaat
    15361 cccctgtccc catccccggg ggtccttcgt gccgaaggag gcgccccgct gcgggtgggg
    15421 gcggggccta ccccgctccc agaagggaaa agcgctcctc ccactcctcc tcgacccgga
    15481 ggagaagcgg gaggtgccct tcgaggcggg ggtcctcttc cgacagcctc cgggcctcct
    15541 ggcgggccat ctccaagagg gccccgtccc gccccagatc cgccactcga aagtaggcca
    15601 ggccgtgctg gcgcaccccc cccaggtctc cgggcccccg gaggcgcagg tcttcttcgg
    15661 ccaaaaagaa gccatcctcc gaagcgcaca gggcctccag gcgtccccgc ccctcggggg
    15721 tggaaggatc tcccaccaat agacacaccg aggcgtcctt cccccgcccc acccggcccc
    15781 gtagctggtg cagctgggcc agaccgaaag aaggggcgtc ttccaccacc aggacggtgg
    15841 cccccgggac gtcgatgccc acttccacca ccgacgtgct caccaaaaga gccaggcgcc
    15901 ccgtggcgaa gtcctccagc acccgggcct tttcccggga ggggagtctc ccgtgcaata
    15961 accccacggc gtgcccggcg aggacctctt ggagggcccg gtatcgccgg gtcaccgccg
    16021 ccacgctccg ggagggattt tcgctctcct ccaccagggg gcagatccaa taaccctgac
    16081 gccccgcatc gagctcccgc cgaaggacct ccagggcctc gtcccgtcga tcttggggga
    16141 tccagcgcgt caccaccggc ttcctccccg ggggcttttc atccaacacg gagacgtcga
    16201 ggtcgccata ggaccccaat aggagggtgc ggggaatggg ggtggcggtc atcaccagca
    16261 cgtggggctc gatccccttt cccgccaggg cggcgcgctg gaggacgccg aagcggtgct
    16321 gctcgtccac caccaccagg gcgaggtggc gaaaggtcac acctttggcg atgagggcgt
    16381 gggtccccac caccacccgg gcctcccccg aggccagggc ttcccggacg cggcgccgct
    16441 ccttttccgc cccgcccccc aacaccagca ggggctccca tcccagaggg gccagaagct
    16501 ggcccagacg atctccgtgc tgacgggcca acgcctccgt gggggccaaa agcaccccct
    16561 ggctccccgc cgccgctgcc gccgccaggg ccgccgccgc caccacggtc tttcccgacc
    16621 ccacgtcccc ctggagcagg cggtgcatgg gcaccggccg ccgcagatct tcccggagct
    16681 cctcccaggc ccgtcgctgg gcccgggtca ggcgaaaggg cagccgggag aaaaagtcct
    16741 cctgccacgc cccatccccc gccaggggaa gggcggcggc ggccctccgc aggcggcggc
    16801 gcagggaaaa ggccacctgg aggaggaaga actcctcgaa gaccaaccgt cgccgggcct
    16861 ggcgccattc ctggtttccc cggggaaagt gcatctccct cagggcctga tccagggaca
    16921 ccagggccaa acggcggcgc agatcctccg gaagggggtc ggggggaagg ggggctccgg
    16981 gggccaagag cccctggatc aagtggcgca ggcgacgctg ggagagcccc gccgtgccgg
    17041 gatagagggg taggatgccc cccgaaggaa cttcgccggg ggcaaggacg tcaaaatccg
    17101 ggtggaccag ctgccctccc ccggttcccg cctcgcagcg gccgtaaaag agggccctcg
    17161 tccccggagg aatctggcgc tccagccctc ggcgggaaaa ccacaccgcc gatccccggt
    17221 ctccccggtc tcccaccagc gtcgcccgga cgaggagcag cccctccctg cgggtgggga
    17281 cccgttccag gcgctccacc cgggccagaa ccgccccccg ttcccccggc accatctccc
    17341 cgggggaacg gatcgtgcgc cgatcctcgt actttcgggg gaaaaaaaag agcaggtccc
    17401 tcacggtccg caccccccgc cgggccaaaa gggcccccag acgctccccc acgccccgca
    17461 gggagcgcac cggagcgcca aaatcctcac cccgggggag cggcggggca atccccggag
    17521 gatgggctcc cttagggggc atcacgaagg tttggcagga tccggacagg cccccggaga
    17581 gaccgcccag gccgcctccg acgaccgggc gatcttctcc tgggcgtcga gcaccatgcc
    17641 cagcttcgac aggagctccc gggtctccgc caccgcctga cgccgcaggc acaccaggcg
    17701 ctccagctgg cgcgccgcgt cctcctcgcc ctggcgcagc cgaaccaccg ccccttgggc
    17761 ctcttcccgg gcctcggcca ggatcttttc cgcctgctcc cgggcctcgg ccaccagagc
    17821 ttccctctcc cgctcggctt tttccccgat ctcccgcgaa gtctgctccg ccaggagcaa
    17881 cgcccgctgg agggcctcct tttgcgtggc atactcccgc aattcatctt cggaggcccg
    17941 ccagcgccgc tcgtgctcct ggaccagctc ggcgtactgc tggagcgtct ccgccacccg
    18001 gtccaagaag tcgtccacgt cccctcgatg gtatccccgg agctcccggc gaaactcctg
    18061 attcgccacc tccagggcgg tcaggagggt cgtcatatcg tcagctcccc gtcggtggcc
    18121 agggctttat cggaccactc ctccaccagg gcgcggtggg acatggccaa aaagaccccg
    18181 ggcaccaccc gcatcaccgc ccccttggtg ccataggcca cgccgcagat gaagtccagg
    18241 gcgctctgtc ccagggcccg ctccaccagg cgcaggtcca ctaaaaccag ctttccttgg
    18301 tgcaacgcct cggcgacctc ctcgcgccgg tgcacgcact gcaacccccg caccaccacc
    18361 agcaccgcct cggggggagc gggctccaac acccggcgct tcctttcctt ccactgctcg
    18421 tcgtcctctt cgaactcctc ttccacttcc tcgaggatgc ccaaccgggc caggagcttt
    18481 tcaaacaccg tcccgccctc ctttttattc cttcggccga gccaaaaggt ctccgtcgtc
    18541 gcccccccgg ccttccgggg gcccgaagat cgccgatccc acccgcacca gagtgcttcc
    18601 ctcctggatc gcctccgggt aatcatcgct catccccatg gacagaaccc ctagggagag
    18661 ccccgacccc ttcgccaggc gatcccggag ctccctcaag gcggcgaagg cccggcggcg
    18721 ctcctcgacc ctgtcggtga ggggggccat ggccatgatc ccctccacgg ccaggagggg
    18781 acagcggcgc tggacggcct ccagcagatc ggccgccgcc cggggatcgg cccccgtctt
    18841 ggccgattcc tccgagatat tgacctccaa cagcaccgtc acgccccgcc ctcgctcctc
    18901 gcagagccgc tggagggtca gggccagggg ctccccgtcg aggctctgga tcgcgtcgaa
    18961 gatctccagg gcccggcggg ccttgttacg ctgcagatgc cccaacagat gccagggcac
    19021 ccggggtgcg ttcgggggcc aggcccgggc cttttccccc gcctcctgca cccggttttc
    19081 ccccagcagg tccacccccg cctccgccgc ctgcctcatg gcctccaagg ggtggtattt
    19141 ggacaccgcg cacaggcgca cctcccgggg atcccggccg cagctccggg ctgcctcctc
    19201 cacgcgggcc cgcacccggg ccacccgctc ccccaggggc gtcgccgccg agaggagtcc
    19261 ctccctttcg cttccaccgt atgctaccac agtttcaccc tcccctcccg ggctccctcc
    19321 gccttttcca ggaccagctc ccccgccgcc aacccctcgg tgaccaaaaa acgccccccc
    19381 ggcaggggaa tgccctgcac gtccctcagg cgcagctccg accccgtcac gacgaagacc
    19441 acctgccttc cccccgtgaa ggtcacggcc tcctcgggaa tcgccgtgcc ctcctgctcg
    19501 cgccccaaaa ggatcaccgc caggcggcgg ccgggcaaca tccccggggg aaagaccggc
    19561 agggtcccat agatcttctc ccgaacgccg taggtctggt gggcccggag ttccatcatc
    19621 accggccgat cccccgaggc caggcgcacc ttcacccttc cccgccggat ctcttccgcc
    19681 acggcggggg taaggggaag gtaggcgatg cagcgaagtt cctggggctg gtagaccaat
    19741 ttgcccaccc cctgcccccg ggtcaccgga gacccctcgg cgatggcctt gggggaaggg
    19801 gggtcgggga gcaccccatc ccctcgccac agggaagaaa agccccactt cccctcctgg
    19861 ccgtccaggt aggggacaaa gtatcccggg gcaggggatc gcaccaccac cgcgccgccc
    19921 ccgcccttta ccgtggccaa cgcatctccc cgggccaccc ggcatatgcg ccccttcccc
    19981 gccagggaaa gggtccccgt cgccggagac gggatcacct cttccctcca gaccagggcc
    20041 agttcggcca agtgctcctc ggcgtagcgc tcgggacgcc cccaggtcac ccgggggtgg
    20101 ctctcctggt agtgacgaaa ccaggagacg tagaccgcca cccccccgga gaccataaaa
    20161 accaccatcg ccaggtaggc cctcatcttc caggagagac ggcggggagg ggcctccgga
    20221 gctttccccc gggatccccg ggggagcaat ttttttaaaa aaaaccgccc caagctctca
    20281 ccctccctag gccctctaaa agccctctgt cccctcggaa aagcccccaa gccccggccc
    20341 ggaaagctcc ccctcccttc gcgaaggggt ccgccgaggc ggggctcccc agggctcgga
    20401 tccccccggg cgccgcagga cgatggctga ggttctaacg tcggtcaccg gtccccgggg
    20461 ggatcttcgc gctcaagaga gccccagata cgtcaggacc gcagacaagg acgacaccac
    20521 cccgtgggcc cccgcctcct ccagctcccg ggggccaaag gatccctggg cgatccccag
    20581 ggcccccatc cccgccgccc gggcggcccg catgtctcca tcggtgtctc ccacgtacca
    20641 ggccccctcg ggagaacggg aaagaatctc cagggcccgc agaagtagat cgggttcggg
    20701 ctttcctcga actccatcct cgagccccac caccacgtca cacaagggta ggatccccac
    20761 ccgctccacc acgcgccggg catagcgccg gttcgtcacc acccccgtgc gcatcccctc
    20821 gtgccgcagg cgcgtcagca cccccggggc atcgggaaag agtttcatcc gggagaattc
    20881 atcctctcgg tagtggtctc cgtaccacgc gaggagctcg ggatccccgc gcccccacaa
    20941 ccgcatacag gcctcggtga agggaaggcc gatggtggcc cccacctcct gggaggtcac
    21001 ggcgggcaag cccttgtcct ccgccacccg gttgaaacag tacgtgatgg cttccacgct
    21061 atcggccagc gtcatatcga agtcaaaaag caccacttgg gccttcatgt cccctctcct
    21121 tccttcttgg gaaagcccgg caaccccttt agggctctag agccccggga ggcgccgttg
    21181 ctctcccccc tcctcggcgg gggccaggcc cagcatgccc ttcaggagag ccgccccccg
    21241 caccgccccc tcccctccac agttattgaa catgacaaag accttgccat cttgggaggc
    21301 ctgggcccac acccggtctc gccagggacg gagctcctcc ggggcgtaga ggtaacggaa
    21361 acgctcctcc accgaggccc cgggacggct ccaggcctcc gccgcccggc cgtggaagcg
    21421 cgccaccgtc ccccaggagg cggtgcgggg ccactccggg ggcatagtcc agggcagatc
    21481 gggctcgtcc acggccacgt aggccacgtt ctccctccgc aaaaggtcca ggaggcgccc
    21541 ccggtgggga tcgtcccacc aagagcggtg acgcacctcc accgccaggc gcaggggacg
    21601 ggccaaggag gccaaccgcc gcacataggc ctccaggcgc cccccggggc gcacccaggg
    21661 gggaaattga aacagaagat accccatacg ccccatgcgg tgcaaggggg ccagggcttg
    21721 gacgaagcgc tcccagaggg aaaggcgcgt ctcccgggga aggtcgtcca gggtaacctg
    21781 ggaagccccc ggggcaagcc cctcccgggc ccagaggggg agggcccgca ccggcacccg
    21841 gtgccaggtg aaaaggccaa aggccttgat gttgaagaga aagcccgggg gggtgcgaga
    21901 aacccaccgg aagatccatt cctcccgggg gagggcgtaa aagacgctgt ccacctccac
    21961 cgtgtcgaaa cgctccgcgt agtatcccag cctcgccccc gcattccgaa gcggcgccgg
    22021 ataccacccg gacccccgta acccccggtc gtcccaggag cacaccccca cccggatctc
    22081 ccccacgctt tactcccctt cgctcgatgc gtgccttcca ttataggttt ctttggaaaa
    22141 ggacgcaaac gccccctccc ccgggacggg gggtcgccct cggatcgcct ccctctgcgg
    22201 ggaagggcgc ctcgcccccc aagggtcttg ccggaaaatc atccaaaaaa tgagctaaaa
    22261 atatctgtat acgacctttg gaggaggtgc gcttcagggg atgcaaaaaa cccttgaaaa
    22321 aaatgcccac ctgtggtaaa gctctctcct gggaggggcg ccaagccccc tcgccatcat
    22381 ccttatcccc atggggggtt tgcctcggtg aacaacggaa aatcggaaaa aacagagcga
    22441 ccggcggaaa tcttcaacaa acacgtcttc tcccaggccg ccatggcgga acgcctgccc
    22501 cgggaggtct tcgaccgcct ccggggcgtc gtggaggggc gggagaagct cgacatccac
    22561 gtggcgggca tcgtggccat ggccatgaag gagtgggccg tggggctcgg ggccacccac
    22621 tacacccact ggttccaacc ccgaacggag ctcaccgccg agaagcacat ggccttcctc
    22681 aacgtcaatg acgcaggcct gcccttggag ttcttctccg ggggggagct catccagagc
    22741 gagcccgatg cctcgtccct cccctcgggg ggcatgcggt cggtcttcga ggctcggggg
    22801 tacacggggt gggatccctc gagcccggcc tttgtggtgt cccactcccg gggggggacc
    22861 ctgtgcatcc cctcggtgtt ttgctcctac gacggcactc ccctggacat gaagaccccc
    22921 ctactcaagg ccatcggcgc cctggaggag cgggccctgc gcatgatccg cctcttcggc
    22981 aaccgagggg tacgctgggt ccacgccacc gtggggggag agcaggagtt tttcctggtg
    23041 gacgaggccc tggcctcccg gcgtcccgac ctggtctttt gcggccgcac cgtcatgggg
    23101 tgcccccctc ccaagggaca gcgcatgggg gaccactact tcggggccat ccatccccgg
    23161 gtgctggagt tcatggaaga cgcccaggac gccctggccc gcctcggcgt ggcgatccgc
    23221 acccggcaca acgaatccgc cccggggcag ttcgaattcg ccccccactt cgccgaggca
    23281 aacctggcgg cggatcagaa ccagatcacc atggaaaccc tccgcaccgt ggcccggcgc
    23341 cacggcttcc tcctcctgct ccacgaaaag cccttcgcgg ggctcaacgg cagcgggaag
    23401 cacatcaact tctccctcca ggacagcgag gggcgcaatc tactgaaacc cgccgggggg
    23461 aacgaacgcc ggggactcca attcctggcc ttcctcaccg ccctgctgcg ggggatgagg
    23521 gaccacgggg ggctcctccg ggcctccgtg gcggggccgg gaaacgtcca tcgcctgggg
    23581 gggcacgagg ctccccctcc catcatgagc gtctttttgg gctccgtcct cacccgcctc
    23641 ctggaggacc tcgaggaggg ggtccaaagt gccatcgacc cccggggaga cctggacctg
    23701 gggctcttcc gccttccggc cctgaggacg gagaacaccg accgcaaccg caccgccccc
    23761 gtggccttca cggggaacaa gttcgagttt cgggcccccg gggcctctca atccatgtcg
    23821 gggcccctca ccgtgatctt cgccctctgg gcccggggga tggatgtcgt catggccgcc
    23881 atccag
//
LOCUS       DAOOER010000028        23574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_2504_length_23574_cov_9.063098, whole genome shotgun sequence.
ACCESSION   DAOOER010000028 DAOOER010000000
VERSION     DAOOER010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 23574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23574
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2504_length_23574_cov_9.063098"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="PL162_05300"
     CDS             <1..315
                     /locus_tag="PL162_05300"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HQF91473.1"
                     /translation="DPEIVGEILVAFQEEYPRLMADLRRGVAQGDPEMVRERSHALKG
                     ICATLGAARARDEAHVLEKMGKGGDLAGASELLGRFEMIMQKVCDELTLVRDELASAK
                     NP"
     gene            287..1384
                     /locus_tag="PL162_05305"
     CDS             287..1384
                     /locus_tag="PL162_05305"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HQF91474.1"
                     /translation="MSWLRRKILEMIPGPCGARREGFIRKVWRESPRRRGLFLGVGLF
                     LGVGSLGGAAEGAPVPLLGVAPRVLQGTAGCALVMDLHSGEILDGWHLAKAFGGSYGA
                     SSVLKPVVAYGALRAGVIDEDDRLGGQDDALLKKYGSSLRPYLRPGGAPLSFPAAMAL
                     SDNAFFYEAGGRLGEGEVRRLFIAFGYGQPSGATGCGPEASGTLATRLSGVHALHFLG
                     WGSPRTRVTAAQVLVATAAFGNGGKLLRPFCPPGELRVRGLLDDPEATAAVRRALRRV
                     VTAGRGKPAAVPGCQVFGKTGSVVDALTGRRLGVFAGFTEGLGRDVALVVVVEGASSD
                     RAAALAGKLIRELRKALGGPGQDKKGGRKSP"
     gene            1381..3534
                     /gene="ppk1"
                     /locus_tag="PL162_05310"
     CDS             1381..3534
                     /gene="ppk1"
                     /locus_tag="PL162_05310"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HQF91475.1"
                     /translation="MKPAKHDPQGEKDREEGGEAGGGDPGRFLNRELQWLAFNRRVLE
                     EAQRESHPLLERVKFEAIFASNLAEFFLIRFSGVHRQILRGVAVPSPDGRTPAEQIRL
                     LREAILPDLAEHRRIWKEDLIPLLRDEGISVEPFEELREEEKQYLGDYFDREIFPVLT
                     PQAVDPGRPFPHISSGSLNLLVALKDEKGNERHARVKLPRAFPRFVPVPDVDGGGGPR
                     IERFVWLEDVVAASLATLFPGYTVDQAYPFQLLRDADVEIQEDEAPDLLATVAEGVEQ
                     RYFGMPVAILVHQSTPERVVRFLADHLELRPYQIFPVDGRVCMGDLMELYALDRRDLK
                     DVPFTPALPGDLAKGESFAEVLRWRDQLLFHPYDSFSPVVDFLTWAARDPQVLAIKMT
                     LYRVGHNSPVVEALMDARHHGKQVAAMVELKARFDEKNNIVWARALERVGVHVVYGLV
                     GLKVHAKMCLVVRREAGGIRKYVHLGTGNYNPSTASVYSDMGLFTADEDIGEDVASLF
                     NVITGYARQKDYRKLLVAPGALRGEMRRRIDREVERHRRHGDGYLAFKMNALVDPECI
                     DALYRASQAGVPIDLQVRGTCCLRPGVPGLSETITVTSIVGRFLEHVRMYYFRAGGGE
                     DEELFLGSADLMERNLDRRVEVLFPIQDPVLRRVLCDEVLQVHLKDTARARLLQPDGS
                     YVPRHPREGEPGVHAQRRMVENRGIWHIPAPGDGI"
     gene            3531..4508
                     /locus_tag="PL162_05315"
     CDS             3531..4508
                     /locus_tag="PL162_05315"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HQF91476.1"
                     /translation="MTPESFVLDSVLERLIPLRAEEEGVRLAEDPEYLHRMRVATRRL
                     RSALPLLAESVSAGDLALWSREIRRFTRALGRARDGDVQIIFLKELIDTWEDRALRPG
                     LERLLLRRSQRRDRLQKGLIRELDRIFRRGGALETLEGEIRRQRVAQALGETPPELAP
                     LRRRGATLLLLRLEALEGGSAALGRPGDGDGHHALRILAKKLRYTLELFAPLMPPTGE
                     AGALLRRLKDLQGILGDLHDCDVWIQDLPLFIRDERRRTEIYQGHLRGFSRIERGLMA
                     FWQDRRREREILFSRSREMWRSWGEDRVTERLREAFRALVEEREGGEER"
     gene            4505..5836
                     /locus_tag="PL162_05320"
     CDS             4505..5836
                     /locus_tag="PL162_05320"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HQF91477.1"
                     /translation="MKIAVLGDIHGNLPAMEAVLRDAKQRRAASVWCLGDLVGYGPFP
                     DEVVAAVREGFILSLRGNYDSKVLRIPTRDPAWFREKGEEKARVFSWAWEHLSPGSRE
                     YLASLPESLRLTVEGYGVLLVHGSPASPREHLSPDTPEERLAALGAECGAAVVLCAHS
                     HVAFARRGGKTLFINPGSVGRPDDGDPRASYALVTFRRPEVEVAFHRLPYDVDRCVEA
                     VRSHGLSEDFARIFQEGRSLDGIRESAPPSGDAVRRQVVAAAEKYVDMQEHHLQVASL
                     ALALFDDLGELHALGEEERDLLEYAALLHDIGWCEGRARHHRWTEKLVMEDDALPFSR
                     RQRRMVACIARYHRRAFPEESHETFGALAEEDRKKVCALAAILRLADALDASHLDLVE
                     GLSCQVGPKKVLVSCTAGQPPQGEDEAWARKGALFRSFFGREAELRWTLRS"
     gene            complement(5859..6869)
                     /locus_tag="PL162_05325"
     CDS             complement(5859..6869)
                     /locus_tag="PL162_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQF91478.1"
                     /translation="MTPLLSAVGLSVGYGGRRPRVVAPDLSLSLEEGSFTALLGRNGA
                     GKSTLIRTICGLQEPLGGEVRLRGVALARHSRRVLARIVGVVLPDRTFPRGMTVYDLV
                     ALGRHPHTGWQGGLALRDREAVDQALGALDLVPFAPRRLGELSDGERQRAFIARALAQ
                     EPALLVLDEPTAFLDPSHRMEVLHLLRDFAHLRGMGVLVALHDVGGALATADRLWILD
                     QGSATEGMPEDLALRGELERTFSSDRWRFDALGGGFVPRQKQKIRGMVGLSGESPGRE
                     WTERALARARYAVAPEAPVSIHVALTPGGWRWVLGGGGEGFTSLEGLLGGLASREEPP
                     RL"
     gene            complement(6866..7861)
                     /locus_tag="PL162_05330"
     CDS             complement(6866..7861)
                     /locus_tag="PL162_05330"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HQF91479.1"
                     /translation="MALIGAVAGGAFVLHLGHGAVALSWGEVWGILLGSGQGSSQDII
                     VWQFRLPRAVTALAAGASLGVAGLLMQTLFANPLAGPYVLGVDAGASLGVALAVMGLE
                     VMGPSWEALATAGSFTAAWVGAALVLLGILSVARQVRSGTVLLLVGILFGFAANAVVS
                     ILVALAPSEKVHGFITWSMGSFAAAPWPRVRLLLLGSLGGLLVAWGLAKSLDALLLGE
                     DYAATMGVSPGRARRRVLLATALLAGSAAACCGPVAFLGIATPHLCRGILRTEGHRWL
                     IPSCAAAGGALALGADGLTRLPGGAVLPLNAVLALVGVPVVAWVLFSSAREGGAS"
     gene            complement(7954..9171)
                     /locus_tag="PL162_05335"
     CDS             complement(7954..9171)
                     /locus_tag="PL162_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQF91480.1"
                     /translation="MKGLGVKALGQKGAATERKPPRPGRSRWGLLALLAWTLGWGGIL
                     SPGEPGLAEEPLRHAALFSVEERPEGILVTNRIPWPGATEEMAWLLQPAASADALPGI
                     PGARRVRVPVSRVVVTSMPAVACMEALGALDALVGVGGGRYCYSPAARARAPVEVGPA
                     EGMGAAFDVERLMALSPDVVFAYAYTAEERAGLDRLEALGIPVVLASEFLEETPLGRA
                     EWIRFFALFLGRQREAEEFFDQVDRHYQRLRLDLASVDHRPRVMAGTPLAGSWHVLGK
                     ESWLGLLIDHAGGSYVWGDIPGRRAVYMDAEAIFAKGAEADLWIHCGPWLSREQALRE
                     EPRAALFRPFREHAVWNNDARSFPGGGNDFYEGGALRPDRILEDLALIFHPERFPGGT
                     PFYYRPLLSEDRR"
     gene            complement(9174..11150)
                     /locus_tag="PL162_05340"
     CDS             complement(9174..11150)
                     /locus_tag="PL162_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQF91481.1"
                     /translation="MIKAFRDRGHAPRSWGFILLLGAWLLGAPPGAALDLPLVTVTAS
                     PWEEASRATPGTVTVLRPEESEGEGKTLGDLLEQSAGVHVIRVEGRGGYVVASIRGST
                     GAQVAVYVDGVLMNLGGEGAVNLATIPADTVERIEVYRGFVPGLFGASGLGAVISVHT
                     RRPRGFEGAAAVELEDFGTWKTSLRYGNDLGSGRYFAAVTLGGTAGDFPYHNDHGTFW
                     NLTDDYEARRRNNGARDRNVLFAWEDRSWRLRGEYLDQHREIPRRAPGNDRPGDPPGP
                     ELDTVRTSLGIGQEFSGERSWGWQAQVLRQDKEFFDPRDPRTLPAPQATWNHHRTERF
                     SLSTHRTQPLGRGSLTFAGEAFRESLRVEGDMVARYRLRSRYDQEVLNLALEGSFPLG
                     SRGRVVPSVRWNGVDGTGHLSWSLGAEWDLTGGWGAHLSYGRAFRAPTLYERFGDGAL
                     LLPRPNLRWEDGIQWDAGVRYEASMGKTRFRGSMTYFHRDVTDLIEFVMANAESAYYD
                     NIGQSTVDGVEVEGAWALPRWQVNLAYTWMRSENLSPGARYGKPLPNRPTHAAHLRVV
                     HDLSDQLSAFVEGKFVGGNAFDQTGDLAMSDLTTVDLGISWTRQDGGVVTAGVRDLFD
                     AASEARLVPFKGPEQMPWYPSAGRTWFLSLRWSW"
     gene            complement(11147..12376)
                     /locus_tag="PL162_05345"
     CDS             complement(11147..12376)
                     /locus_tag="PL162_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91482.1"
                     /translation="MRWRKAVGGILGVLWLAGSAGAENPSVAYVTMDFTTMSGLRGVT
                     SGDGATPLGATGGGDPRYMAFVKNGAQKILYREYQTGDAPDTVTIFDPTNWDTPEANT
                     TLGKNIGGAAVSGSFLLLANYYNDEGHSYLTKHDMTDPHYRQVDARNLDDGDGANFSE
                     YVKDVLAWKGSIYALVERRDNVYPPHYAPSTLVRFNANLEETGSVDLGQNASTMALEK
                     DTIYVAVIGGPRDVASGDTALVAVDPATMAKTVLGTSRDLPAPYAFDHVGVAPDGTIF
                     VTGYNNGAGTWPNPSKLFTAAPGTPRTFVDRGTQEGWVSKGVWDPSGEVFWVVNHGSG
                     RGDSVLEAYDTAGNRVLRISETTLGGAVYDAVPLTAAAPTQAPSGGSGGGGCATGALP
                     TPLALLLLAPALLLRRP"
     regulatory      complement(12532..12691)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(12841..13047)
                     /locus_tag="PL162_05350"
     CDS             complement(12841..13047)
                     /locus_tag="PL162_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91483.1"
                     /translation="MEILEGPLDISRCHEVPFCGASAQGCALRDILEDVNDAILRTLE
                     GVTLADFVQRQRAKELLADDASGA"
     gene            complement(13140..13583)
                     /locus_tag="PL162_05355"
     CDS             complement(13140..13583)
                     /locus_tag="PL162_05355"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HQF91484.1"
                     /translation="MKLSTRSRYGLRAMMDIALAREGTLVMAKDIAERRQLPVAYLEQ
                     LLGQLKRGGLISSMRGSRGGYALARPAEAITLAAVVEILEGPLDISRCHEVPFCGASA
                     QGCALRDILEDVNDAILRTLEGVTLADFVQRQRAKELLADDASGA"
     gene            complement(13791..14885)
                     /gene="mnmA"
                     /locus_tag="PL162_05360"
     CDS             complement(13791..14885)
                     /gene="mnmA"
                     /locus_tag="PL162_05360"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HQF91485.1"
                     /translation="MQNGPMTMKGCAMEKFLPARPRVAVLMSGGVDSSVAAWLLREEG
                     WEGVGVTLRLFSPGDDAAPGVCRFLGMPHVFFDARQLFEDRVLSPFRRGYARGETPSP
                     CVGCNAALKFGLAWEVARSLPGVSFIATGHYARILHRPEGVRLARARDLRRDQSAFLY
                     ALPPEVLPRTLFPLGELSKEEVRARARRAGLPTALRRDSMDLCFAGVEGYRSVLDPAL
                     LRRPGPILDEEGHELGIHPGIVHFTPGQRQGLGIASPVPLYVLRVDPLRQALVVGPRE
                     RALAREVEARQPRLLAPDLIFPGARVWGKVRAPGPLTPGTVELLTEDRLRCRFDEPVF
                     APAPGQHLVLYGEEGNVVAGGGEIVAPRSA"
     gene            complement(15141..16097)
                     /gene="cysK"
                     /locus_tag="PL162_05365"
     CDS             complement(15141..16097)
                     /gene="cysK"
                     /locus_tag="PL162_05365"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HQF91486.1"
                     /translation="MSRIFDDHAQSMGNTPLVRLHRVAGKGVTVLAKVEGRNPTSSVK
                     CRVGAALIWDAEARGVLTPEKTIVEPTSGNTGIALAYVAAARGYDLTLVMPENMSVER
                     RKVLALLGAHLVLTPEREGMAGAVRRVEELAAEDPDHFFLPQQFRNPANPAIHEATTG
                     PEVWRDTDGAVDVLVAGVGTGGTLTGISRHIKKTQKKAIISVAVEPEGSAVISRHLAG
                     LPGDPGPHGIQGIGAGFLPETLDLSLVDRAETVSTEEALEFARALARKEGILAGISSG
                     AAVAVASRLAAREEFREKVFVVILPDAGERYLSTPLFDGLEI"
     gene            16350..17186
                     /locus_tag="PL162_05370"
     CDS             16350..17186
                     /locus_tag="PL162_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HQF91487.1"
                     /translation="MLERWMAGLEKDVERRRGYDVVPWALCQEVARREGIPLGQVERI
                     VLRRGLVPSRYERNVGTLGCEGQLRLLEARGAVVGCGGLGGVVVELLARAGVGTLVLV
                     DGDTFADHNLNRQILCREEDLGRPKVRVAMERVRSVNSAVEVVAVETFLTEENAPGIL
                     AGCDVAVDALDGQSSRRVLFRACAALGIPAVHGAIGGFWGQVSTVIPGDRSALDLWGV
                     DDPDRGAEEERGNPPCTPAAVGALEAAEALKILAGVGEPLRGKLLWGDLGSQEWRVLP
                     LG"
     gene            complement(17209..18288)
                     /locus_tag="PL162_05375"
     CDS             complement(17209..18288)
                     /locus_tag="PL162_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91488.1"
                     /translation="MRKNARPPMPQTPKATKQHYASAPPDRPKEPEAMQWRNLFTLAD
                     EMRRTEPWKWMEGFAPFAIRIPGTDRIYHCRFTGPGGALPGFVALRGTRGLCHHLLAL
                     GGMPPHAPERSLTRDGLILSFGTKKTMDKDDRTLMTSLKRFYRGGDVWPRFRSQVPCA
                     PEAPLNAFEAEDFAPLLSQALVVASICRTDPRFCLGPEPAPGRMLLREGTWEEDGGLS
                     WESRSLSFSSEELRRELFPGEDALRELGAGLSDLPLGSGTLSLFLRGPSSDAPEEGPG
                     PSLVLRGSWEGVEEGEFSEPLGSKAAGGSPWDFPLGMLFDIFRSRRSLPGGVTFHPPL
                     LEEALGGFFRSLAEAPRATAPGDGA"
     gene            complement(18511..19368)
                     /locus_tag="PL162_05380"
     CDS             complement(18511..19368)
                     /locus_tag="PL162_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HQF91489.1"
                     /translation="MELIFRATLAQTAPELLNVPHNAQRAAEALARAAERGASLVVFP
                     EMHLSGYGLEKARLSPSHRAALARDVAHALEGLASEGERLGVDYLMSYPIFEDDKIFI
                     AAAYVERGHLRAVHRKLNLVNYGHYTEDRNFDPGNSFTVVSTRLGVMGILICEDLWHP
                     VNAAVCAARGMEVLLAPSAPCVPDPSRARGNMEEWKILLRGTAFSLTTPILCATQGGR
                     EGSMAFNGGSFALSPEGEVLLELPLLEEAEGDVEIDRDALEALRRRRPLLANERHDLY
                     AAAFGSRGA"
     gene            complement(19600..22989)
                     /locus_tag="PL162_05385"
     CDS             complement(19600..22989)
                     /locus_tag="PL162_05385"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HQF91490.1"
                     /translation="MRGPGSAGVGVILLAVMMALFSPLGASGAEEGATVLLGPGDLFG
                     SQMVFIYAGDLWVSQADGTAFRRLCTEGTAVGRPAFSPDGRDVVFSLMNHGNVDGYIM
                     PVSGGTPRRLTAHPGVDRVLGFSADGKEVLLTSDREAALGSSYALFAVPREGGPLRRL
                     SLPQAFDGAFSPDGGLFAYNPLPPAFRIWKGYRGGTISVIHLFDPATRKDTTVPQPSE
                     GCNDVQPQWIGNTLYFLSDRGGEFNIHSFDPATRQVRALTAFDAFPVESIAASPEGRL
                     LFLREGRLGLKDPASEGYQIVPLSLAADLPETRLRFVKGENFIQGFDLSPSGARGVFA
                     MRGDIVTVPADKGDPRALTTSSGVHDRNPAWSPDGRLVAWFADDGPEYVLKIRRQDAR
                     GQEVTVPLLGRGMYFVPAWSPDGKKIAFRDQNATVYWIDVAAAAAAASDDRPLSPDLL
                     KAPVEVCREPLWGSPYKWLELSWAPDSCHLAFATTLTNGLEQAVIHRVDTGQTFPVSD
                     RMADVICPTFDPGGKYLYFFGSTNAGPAKFTLDMSTQDKPIIRSLYMTILAKGVPSPL
                     ARESDEEEPGVLEEASGEEGAPDQGASADQGGEGPAPALSPDAKDRAHAPLSFDAEGV
                     LDRTLPLPIPAGFYSDLRPGKEGTIYYLRLEGTEDGGSTVLLCSWNLEEREEKVLLRG
                     VWDYRVSLRGEKLLVQHDGGWTIGAPGDLASAKAMDLGRVRIPLNPREEWREIYRDAW
                     RLNRDYFYDPQMYGLDWDGVYEKYLPFLDHLPGRRDLDGLLAAMLSELRVGHHYVVGG
                     DFPDQEGEEEEIGLLGADVAPEGGRYRLTRILGGVNWEPSLRSPLTEPGVNVKEGDFV
                     LKVGDREITASEDFFAPFAHTANRLVELTVASDPSGKDSRVVSVVPLDSERALRLKAW
                     VEGNVRWVEERSGGRVSYVYMPNTATAGYEYFNRYFFSQLPRDGLILDDRNNGGGQFA
                     DYVLDALSRRFTTGVTMRSGADYGSPAAYVEGPKAMIINESAGSGGDMLPWAFRKKGL
                     GVLVGKRTWGGLVGITDYPLLQDGTWVTAPNMGLWDERGWIVENVGVSPDIEVEQDPA
                     AVLAGRDPQLETALRVVLEELEKNPPRRPARPAFPVMGPGK"
     gene            complement(23256..>23574)
                     /locus_tag="PL162_05390"
     CDS             complement(23256..>23574)
                     /locus_tag="PL162_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91491.1"
                     /translation="PGVLLSEQTIAAMISPAELSDGSVFSIPLEDGEATFGMGLEVFT
                     WHEQVVPTKNGAISGYSALFLAHPSSKTAVAVLTNLSDSGRAAQRIARKAAALVSSGA
                     LPE"
BASE COUNT         3873 a         7977 c         8065 g         3659 t
ORIGIN      
        1 gaccccgaaa tcgtggggga gatcctggtg gccttccaag aggaataccc ccggctcatg
       61 gcggacctac gccggggggt ggcccagggg gacccggaaa tggtgcggga gcggtcccac
      121 gccctcaagg gcatctgcgc caccctgggg gcggcgcggg ctcgggacga ggcccatgtg
      181 ttggaaaaga tgggaaaagg aggggacttg gccggagcgt cggagctcct gggccgattc
      241 gagatgatca tgcaaaaagt ctgtgacgag ctgaccctgg tgagggatga gctggcttcg
      301 gcgaaaaatc cttgagatga tccccggacc ctgcggggcc cggagggagg gcttcatccg
      361 gaaagtttgg cgggaatcgc cgcgaaggag ggggctattc ctgggggtcg gactctttct
      421 gggggtgggg tctctggggg gcgcggcgga gggggccccc gttccccttc tgggggtcgc
      481 cccccgggtg ctccagggta cggcggggtg cgccctggtg atggacctcc actccggcga
      541 gatcctggac gggtggcacc tggcgaaggc cttcgggggg agctatggcg ccagcagcgt
      601 cctcaagcct gtggtggcct acggggccct gcgggcgggg gtcatcgacg aggacgaccg
      661 cctgggaggg caagacgatg ccctgctgaa aaagtacgga agctccctgc gtccttacct
      721 gcggcccggg ggggctccgc tgtcttttcc cgccgccatg gccctgagcg acaacgcctt
      781 tttttacgag gcggggggaa ggttgggaga aggggaggtg cggcgcctct tcatagcctt
      841 tgggtacggc cagccttcgg gggcgacggg gtgcggcccg gaggcttcgg ggacgctggc
      901 cacccgcctt tcgggggtcc atgccctgca cttcctcggg tgggggagcc cccgaacccg
      961 ggtgacggcg gcccaggtgc tcgtggccac cgccgccttt ggcaacgggg gaaagctcct
     1021 gcgccccttt tgccccccgg gagaactccg ggtgcggggc ctcctagacg atcccgaggc
     1081 gacggcggcg gtgcgccggg ccctgcgccg ggtggtcacg gcgggtcggg gaaagcccgc
     1141 cgccgttccg gggtgccagg tcttcggcaa gacgggctcc gtcgtcgacg ccctcacggg
     1201 gcggcgcctg ggggtctttg cgggatttac cgaggggctg ggccgggacg tggccctggt
     1261 ggtggtggtg gagggggctt cctccgaccg ggcggcggcg ctggcgggta agctgataag
     1321 ggaactccgg aaagcccttg gcggcccggg gcaggataaa aagggggggc gaaagagccc
     1381 atgaagcctg cgaagcacga tcctcaggga gaaaaagacc gggaggaggg gggcgaggcc
     1441 gggggagggg atccggggag gttcctcaac cgggagttgc agtggcttgc ctttaaccga
     1501 agggtcctcg aggaggccca gcgggaatcc caccccctgc tggagcgggt gaagttcgag
     1561 gcgatcttcg ccagcaattt ggcggaattc tttcttatcc gcttctccgg ggtgcaccgc
     1621 cagatcctcc ggggggtggc ggtgccctcc cccgacggcc ggacccccgc agagcagatc
     1681 cgcctccttc gggaggccat cctccccgac ctggcggagc accggaggat ctggaaggag
     1741 gacctcatcc ctctcctccg ggacgagggg atctccgtgg agcctttcga agagctccgc
     1801 gaggaagaaa agcagtacct gggggactac ttcgaccggg agatcttccc ggtcctcacc
     1861 ccccaggcgg tggaccccgg acgacccttt ccccacatct ccagcggcag cctcaacctc
     1921 ctcgtggccc tgaaagacga aaaaggcaac gagcgccacg cccgcgtcaa gctcccccgg
     1981 gcgtttcccc gcttcgtccc cgtgcccgac gtcgacggag ggggggggcc ccggatcgag
     2041 cgcttcgttt ggctggaaga cgtggtggcg gcgagcctgg ccacgctctt tcccggctac
     2101 acggtggatc aggcctatcc cttccagctc ctccgggatg cggacgtgga aatccaggaa
     2161 gacgaagccc cggacctttt ggccaccgtg gccgaggggg tggagcagcg ctacttcggc
     2221 atgcccgtgg cgatcttggt gcaccaatcc accccggagc gcgtggtgcg ctttctggcg
     2281 gatcacctgg agcttcgccc ctaccagata tttcccgtgg atggccgggt ctgcatgggg
     2341 gatctcatgg agctctacgc cctggaccgg agggatctga aagacgtccc cttcaccccg
     2401 gccctccccg gagatctggc taagggggag tcctttgccg aggtcctgcg gtggcgagac
     2461 cagctcctct ttcaccccta cgacagcttc agcccggtgg tggattttct tacctgggcc
     2521 gcccgagatc cccaggtgct ggccatcaag atgaccctct accgggtggg gcacaactcc
     2581 cccgtggtgg aggccctcat ggacgcccga caccacggca agcaggtggc cgccatggtg
     2641 gagctcaagg cccgcttcga cgagaagaac aacatcgtct gggcccgggc cctggagcgg
     2701 gtgggggtgc acgtggtcta cggcctcgtg gggctaaagg tacacgccaa aatgtgcctc
     2761 gtggtccggc gggaagcggg gggcatccgg aagtacgtcc acctggggac gggaaactac
     2821 aacccctcca ccgccagcgt ctacagcgac atggggctat tcaccgccga cgaggacatc
     2881 ggcgaggacg tggcctccct gttcaacgtc atcacgggat acgcccgaca gaaggactat
     2941 cgcaagctcc tggtggcccc cggggccctc cggggggaga tgcggcggcg catcgaccgg
     3001 gaggtcgagc ggcaccgaag gcacggcgac ggctatctgg ccttcaagat gaacgccctg
     3061 gtggatccgg agtgcatcga cgccctctac cgggcctccc aggcgggggt ccccatcgac
     3121 ctccaggtgc ggggcacctg ctgccttcgc cccggagtgc cgggcctcag cgagaccatc
     3181 accgtgacct ccatcgtggg gcgctttctg gagcacgtcc gcatgtacta cttccgggcc
     3241 ggaggggggg aggacgaaga gctcttcctc ggcagcgccg acctcatgga gcggaacctg
     3301 gaccgccgag tggaggtctt atttcccatc caggatccgg tccttcggcg ggtgctgtgc
     3361 gacgaggtcc tgcaggtcca cctgaaagat accgcccggg cccgcctcct gcagccggat
     3421 gggtcctacg tcccccgaca cccccgggaa ggggagccgg gggtgcacgc ccagcgccgc
     3481 atggtggaaa accggggaat atggcacatc cccgccccgg gggacgggat atgactccgg
     3541 aaagttttgt cctggactcg gtcctggagc gcctgatccc cttgcgggcc gaggaggaag
     3601 gggtgcgcct cgccgaagac cccgagtacc tccaccgcat gagggtggcc acccgacgcc
     3661 tgagaagcgc cctccccttg ctggccgaaa gcgtttccgc cggcgacctc gccctctgga
     3721 gccgggagat tcggcgcttc acccgggccc tgggccgggc ccgggatggg gacgtgcaga
     3781 taattttcct caaagagctc atcgacacat gggaagaccg ggccctgcgc cccggcctgg
     3841 agcgcctgct cctccggcga agccagcgga gggatcggct ccagaaggga ttgatccggg
     3901 agctggaccg gatcttccga cggggcgggg ccctggagac cctggagggc gagatccggc
     3961 gccagcgggt ggcccaggcc ctgggggaga ccccgccgga gctggccccc ctccggcgac
     4021 ggggggccac gctcctcctc ctccgcctgg aggccctgga ggggggatcg gccgccctgg
     4081 gacgacccgg agacggagat ggacaccacg ccctgcgcat cctggccaag aagctgcgct
     4141 acaccctgga gctctttgcc cccctgatgc cccccaccgg ggaggcgggg gccctcctcc
     4201 ggaggctgaa agacctccag ggaattctgg gagatctcca tgactgcgac gtctggatcc
     4261 aggacctgcc cctcttcatc cgggacgagc ggcggcgcac cgagatctac caaggccatc
     4321 tgaggggctt ttcccggatc gaaagggggc ttatggcctt ctggcaggat cggcgccggg
     4381 agcgcgagat cctcttttcc cgttcccggg aaatgtggcg ctcctgggga gaggatcggg
     4441 tgacggagag actccgggag gcctttcggg cgctggtgga ggagcgagaa ggaggcgagg
     4501 aacgttgaag attgccgtct tgggggacat ccacggcaac cttcccgcga tggaagcggt
     4561 gttgcgggac gcgaaacagc gcagggccgc ctcggtgtgg tgcctggggg acctggtggg
     4621 gtacggcccc tttcccgatg aggtggtggc cgccgtgcgg gaggggttca tcctctccct
     4681 ccggggcaac tatgacagca aggtgctacg gatccccacc cgggatcccg cctggttccg
     4741 ggagaaaggg gaggaaaagg ctcgggtctt ttcctgggcg tgggagcacc tctctcccgg
     4801 atcccgggag tacctcgcct ccctgccgga gagcctgcgc ctgacggtgg aaggttatgg
     4861 ggttcttttg gtccacggca gccccgcctc tccccgggag cacctctccc ccgacacccc
     4921 cgaagagcgc ctcgccgccc tgggggcgga gtgcggcgcc gccgtggtgc tctgtgccca
     4981 ctctcacgtc gccttcgccc gccgaggggg aaagaccttg ttcatcaacc ccggcagcgt
     5041 gggacgcccc gacgacgggg atccccgggc ctcctacgcc ctggtgacct tccggcgccc
     5101 cgaggtggag gtggccttcc accgcctccc ctacgacgtg gatcgctgcg tggaggcggt
     5161 gcggtcccac gggctctccg aggacttcgc tcggatcttc caggaggggc gttctctgga
     5221 tgggatacgg gaaagcgccc ccccctcggg ggatgccgtg cggcgacagg tcgtcgcggc
     5281 ggcggaaaag tacgtggaca tgcaggagca ccacctccag gtggcctcct tggccctggc
     5341 gctcttcgac gacctggggg agctccacgc cttgggggaa gaggagcgag acctgctgga
     5401 gtacgcggcg ctgctccacg acatcggctg gtgcgagggg agggcccgcc accaccggtg
     5461 gacggaaaag ctggtgatgg aagacgacgc cctgcccttt agcaggcgtc agcgccgcat
     5521 ggtggcctgc atcgcccgat accatcgccg ggccttcccc gaagagagcc acgagacctt
     5581 cggcgccctg gccgaggaag accggaaaaa agtctgcgcc ctggcggcga tcttgcgcct
     5641 cgccgacgcc ctggacgcct cccacctgga cctggtggaa ggactctcct gccaggtggg
     5701 gccgaaaaaa gttctcgtga gctgcacggc gggccaaccc ccccaggggg aggacgaagc
     5761 ctgggcccgt aagggcgccc tctttcggtc cttttttggc cgggaggcgg agctccgatg
     5821 gacgctgcgg agctgacccc ggggcgccct cgacgggact agagccgagg aggctcttcc
     5881 cgggaggcca accccccgag gagcccctcc agcgaggtaa agccctctcc ccctcctccc
     5941 aggacccatc gccacccccc gggggtgagg gccacgtgga tcgagacggg agcctctggt
     6001 gccacggcgt agcgggcccg ggccagggcc cgctcggtcc actcccggcc gggactttcc
     6061 cccgacaggc ccaccatccc ccggatcttt tgcttctgtc ggggcacgaa gccccccccc
     6121 agggcgtcga aacgccaccg atcggaggaa aaggtccgtt ccagctctcc ccgaagggcc
     6181 agatcctccg gcatcccctc cgtggcggat ccctggtcca agatccagag ccgatccgcc
     6241 gtggccagcg ccccccccac gtcgtgcagg gccacgagga cccccattcc ccggaggtgg
     6301 gcgaagtccc tcaggaggtg caagacctcc atgcggtggg agggatccag gaaggccgtg
     6361 ggctcgtcca gcaccagcaa ggcgggctcc tgggccaggg cccgggcgat gaaggcccgc
     6421 tggcgctctc cgtcgctcag ctcccccagg cggcgggggg cgaaggggac gaggtccagg
     6481 gcccccaggg cctggtccac cgcctcccga tccctaagag cgagcccccc ttgccacccc
     6541 gtgtgggggt ggcgccccag ggccaccaga tcgtagaccg tcattccccg gggaaaggtc
     6601 cggtcgggca gcaccacgcc cacgatgcgg gccaggaccc tccgggagtg ccgggccagc
     6661 gccactcccc ggagccgcac ctctcccccc aggggctcct ggaggccaca gatggtcctg
     6721 atcagggtgg atttccccgc gccattgcgc cccaacaggg cggtgaagct cccttcctcc
     6781 agggacaggg agaggtcggg ggccaccacc cgggggcggc gtcccccgta ccccaccgaa
     6841 agccccacgg cggaaagcaa aggcgtcatg acgccccccc ttcccgggcg gagctaaaga
     6901 gcacccaggc caccacgggg acccccacga gggccagcac cgcgttgagg ggcaacaccg
     6961 cccctccggg gaggcgggtg aggccgtcgg cccccagggc caaggcgccc cccgcagcgg
     7021 cgcaggaggg gatgagccat cggtgcccct cggtccgcaa aatgccccgg caaagatggg
     7081 gagtggcgat gcccaggaag gccacggggc cgcaacacgc cgcagcgctt cccgctagca
     7141 gggccgtggc caggaggacg cggcgccggg ccctgccggg ggagactccc atggtggcgg
     7201 cgtaatcctc ccccagaagc agggcatcca gggacttggc gagcccccag gccaccaaaa
     7261 gcccccctaa agaccccagg agcaggaggc gcacccgggg ccagggggcc gccgcgaagc
     7321 tccccatgct ccaggtgatg aagccgtgga ccttttccga cggggccagg gccacgagga
     7381 tgctcaccac ggcgttggcg gcgaacccga agaggatccc caccaggagg agcacggtgc
     7441 cgctgcggac ctggcgggcc acggagagga tccccaggag gaccagggcg gccccgaccc
     7501 aggccgccgt aaagctcccc gccgtggcca gcgcttccca ggagggcccc attacctcca
     7561 gtcccatcac cgccaaggcc acccccaggc tggcccccgc atccaccccc agcacgtagg
     7621 gccccgccag ggggttggca aagagggtct gcatgaggag ccctgccacc cccaggctgg
     7681 cccccgccgc caaggcggtc accgcccggg gcagacggaa ttgccacacg atgatgtcct
     7741 gcgaggagcc ctggccggag cccaaaagga taccccacac ctcgccccag ctcagcgcca
     7801 cggcgccatg ccccaggtgg aggacgaagg cccccccggc gacggcgccg atcagggcca
     7861 tcgccgccca tcgccagacg tcccgagaaa ggacctccct actcgggaga gaggggattt
     7921 tcgggccccc tcggggggat tccgggggga aagtcatcgc cggtcttccg agaggagcgg
     7981 acggtagtaa aagggagtgc cgccggggaa gcgctccggg tggaagatga gggccaggtc
     8041 ctccaggatc cggtcggggc ggagggcccc tccctcgtag aaatcgttgc cccccccggg
     8101 aaaggaccgg gcgtcgttgt tccacaccgc gtgctcccga aaggggcgaa agagggccgc
     8161 ccggggctct tccctcaggg cctgttcccg ggatagccac gggccgcagt ggatccataa
     8221 atccgcctcc gcccctttgg cgaagatcgc ctcggcgtcc atgtagacgg cccttctccc
     8281 ggggatgtcc ccccagacgt aactcccccc cgcgtggtcg atgaggagcc ccagccagct
     8341 ctccttcccc agcacgtgcc acgaccccgc caggggcgtc cccgccatca cccgggggcg
     8401 gtgatccacc gaggcgaggt cgaggcggag gcgctggtag tggcggtcca cttggtcgaa
     8461 aaactcctcg gcctccctct ggcgtcccag gaagagggcg aaaaagcgaa tccactccgc
     8521 cctccccagg ggcgtctcct ccaggaactc cgaagccagc accacgggga ttcccagggc
     8581 ctccagtcgg tcgagcccgg cgcgctcctc cgccgtgtag gcgtaggcga agaccacgtc
     8641 gggggagagg gccatcaggc gctccacgtc gaaggccgcc cccatgccct cggcggggcc
     8701 cacctcgacg ggggccctag cccgggccgc cggagagtag caataccgcc ctccccccac
     8761 gcccaccaag gcatccaggg ctcccagggc ctccatacag gccacggcgg gcatggaagt
     8821 caccaccacc cgggagacgg gcacccggac ccgccgcgcc ccggggatcc cggggagagc
     8881 gtcggccgaa gcggcgggct gtaagagcca cgccatctcc tcggtggccc cgggccaggg
     8941 gatgcgattg gtgacgagga tcccctcggg gcgctcctcc acggaaaaca aggcggcgtg
     9001 gcgcaggggt tcctccgcga ggcccggttc tcccggggac agaatccctc cccaacccag
     9061 ggtccaggcc agcagggcca aaaggcccca tcggctccgc cccgggcggg ggggctttct
     9121 ttcggtggcg gcgccctttt gtcccagggc tttcacccca aggcccttca tcctcaccag
     9181 ctccaccgca gggacaaaaa ccacgttcgt ccggcggaag ggtaccaggg catctgctcc
     9241 ggccccttga agggcaccag ccgcgcctcc gaagcggcgt cgaagagatc tcgcacgcca
     9301 gcggtgacca cgcccccgtc ttgccgggtc caggagatcc ccaggtccac cgtggtgagg
     9361 tcggacatgg ccaggtcccc cgtctggtcg aaggcgtttc cccccacgaa cttcccctcg
     9421 acgaaggccg agagctgatc ggacaggtcg tggaccaccc ggagatgggc ggcgtgggtc
     9481 gggcggttgg gcaggggctt accgtaccgg gcccccggag agagattctc gcttctcatc
     9541 caggtgtagg ccaggttgac ctgccaccgg gggagggccc aggccccctc gacctccacc
     9601 ccgtccaccg tgctctggcc gatgttatcg tagtaggccg attccgcatt ggccatgacg
     9661 aactcgatga gatcggtcac gtctcggtga aagtacgtca tcgacccccg gaagcgcgtc
     9721 tttcccatcg aagcctcgta gcgcaccccc gcgtcccact ggatgccgtc ctcccagcgc
     9781 aagttcgggc ggggcaaaag cagcgccccg tcgccgaagc gctcatagag cgtgggggcc
     9841 cggaacgccc ggccgtagga gagatgggct ccccatcccc ccgtcagatc ccactccgcc
     9901 cccagggacc aggagaggtg tcccgtgccg tccaccccat tccaccgcac cgagggcacc
     9961 acccgacccc gggaacccaa gggaaaagac ccctccaggg ccaggttcaa gacctcctgg
    10021 tcgtagcggg atcggaggcg gtatcgggcc accatgtccc cctccacccg aagggactcc
    10081 cgaaaggcct cccccgcgaa ggtgaggctt ccccttccca ggggttgggt tcggtgggtc
    10141 gagagggaaa agcgctccgt gcggtggtgg ttccatgtgg cctggggcgc gggcagcgtc
    10201 cggggatccc gggggtcgaa gaactccttg tcctggcgca acacctgggc ctgccatccc
    10261 cagcttcgct ccccggaaaa ctcctgccct atccccagag aggtccgcac cgtgtccagc
    10321 tcgggccccg gaggatcgcc ggggcggtca tttcccgggg cccgacgggg aatctcccgg
    10381 tgctgatcca gatattctcc ccgcaggcgc caggatcggt cctcccaagc gaaaagcacg
    10441 tttcggtccc gggcgccgtt gttgcggcgc cgggcctcgt agtcatccgt aaggttccag
    10501 aacgtgccgt ggtcgttgtg ataggggaaa tctcccgctg tgccccccag ggtgacggcg
    10561 gcgaagtagc gccccgaccc cagatcgttg ccgtagcgca gcgacgtctt ccaggtgccg
    10621 aagtcttcca gctccaccgc cgccgcccct tcgaagcccc ggggccgtcg ggtgtgcacc
    10681 gagatcaccg cccccagccc cgaggcccca aagagccccg gcacgaagcc ccggtagacc
    10741 tcgatgcgct ccaccgtgtc ggcggggatg gtggccaagt tcaccgcccc ttccccacct
    10801 aaattcatca ggacgccatc gacgtacacc gccacctggg cccccgtgct cccccggatg
    10861 gaggccacga cgtacccccc ccgtccctcc acccggatca cgtgaacccc cgcgctctgc
    10921 tccaggaggt cccccagggt cttgccctcc ccctcgcttt cctcggggcg gaggaccgtc
    10981 accgttcccg gcgtggcccg ggaggcctcc tcccagggcg aggcggtcac cgtcaccagg
    11041 ggaagatcca gggcggcccc ggggggcgcc cctaaaagcc acgccccgag gaggaggata
    11101 aagccccagg accggggggc gtgcccccgg tcccggaacg cctttatcat ggccgccgca
    11161 acaaaagggc tggcgccagg agcaggagcg ccagaggagt cggcagggcc cccgtcgcac
    11221 acccgcctcc gccgcttccg ccggagggag cctgggtggg cgcggcggcg gtcaggggca
    11281 cggcgtcata gaccgcccct cccagggtgg tctcggagat ccgaagcacc cggttccccg
    11341 ccgtatcgta ggcctcgagg accgaatccc ctctgcccga gccgtgattc accacccaaa
    11401 agacctcccc cgacggatcc cacacgccct tggacaccca accctcctgg gtgcctcggt
    11461 ccacgaaggt ccggggcgtt cccggggccg ccgtgaagag cttgctgggg ttgggccagg
    11521 tcccggcgcc gttattgtac cccgtgacaa agatcgtgcc gtcgggagcc actcccacat
    11581 ggtcgaaggc gtagggggcg gggagatccc gactggtgcc gagcacggtt ttggccatcg
    11641 tggcgggatc caccgccacc agagccgtgt ctccgctcgc cacgtcccgg ggtcccccga
    11701 tgaccgccac gtagatggtg tctttttcca gggccatggt ggaggcattt tgtcccaggt
    11761 ccaccgaccc cgtttcctct agattggcgt taaagcgcac cagggtactg ggggcgtagt
    11821 gggggggata gacgttgtcc cggcgctcca ccagggcgta gatcgacccc ttccaggcca
    11881 ggacgtcttt cacgtactcc gagaagttgg ccccatcgcc gtcgtccaag ttgcgggcgt
    11941 ccacctggcg gtagtggggg tcggtcatgt cgtgcttggt gaggtaggag tgcccctcgt
    12001 cgttatagta gttcgccagg agcaggaagg acccggacac cgccgcgccg ccgatgtttt
    12061 tccccagggt ggtgttggcc tcgggagtgt cccagttcgt ggggtcaaag atcgtcaccg
    12121 tgtccggggc gtctccggtt tggtactccc ggtagaggat cttctgggcg ccgtttttca
    12181 cgaaggccat gtagcgggga tcccctcctc ccgtggctcc caacggggtg gctccgtccc
    12241 ccgaagtgac cccccggaga ccggacatgg tggtaaagtc catcgtcacg taggccaccg
    12301 agggattctc cgcccccgcc gatcctgcca accacagcac tcccaagatg cctcccacag
    12361 cctttctcca tctcatgttt ccttcctcct cgttagaccc gatctgcgga gcttccgggg
    12421 gcaccccccc tagggcacaa aaaagggcgg gaagcgggcc ttgatgccat ccgctcctcg
    12481 ccgcgcgaac gcccccatcg gggaagctct cgtccctcca cgcccgcgca tggaagacgc
    12541 gtgctgtccg gccggtctcc tggcttcagt tcatcctact cccgggcctt ccccatcgct
    12601 gggtggcgtg cgcggtttcg tccctgatac agtggcgggg ccgctccggc gtgacaccgg
    12661 attcccgtac accggacaac cttcgatttt tctgacgcca tatcatcata acatggccgc
    12721 gttctccgtc aagggcaaaa agtcccggga tttacccccg ggccaaaccg gaggcggggg
    12781 taccggttcg ggagaaacgt ttctctttgc gccttcgggc gaggaggcgc gaagggggcg
    12841 tcacgccccg gaggcatcgt ccgcgagaag ctccttggcc cgctggcgtt gcacgaagtc
    12901 tgccagggtg accccttcca gggtccggag gatggcgtcg ttgacgtctt ccaggatgtc
    12961 tcgcagggcg cacccctggg ccgacgcgcc gcaaaagggc acctcgtgac atcgggagat
    13021 gtccaaggga ccctccagaa tttccacgat ttacccccgg gccaaaccgg aggcgggggt
    13081 accggttcgg gagaaacgtt tctctttgcg ccttcgggcg aggaggcgcg aagggggcgt
    13141 cacgccccgg aggcatcgtc cgcgagaagc tccttggccc gctggcgttg cacgaagtct
    13201 gccagggtga ccccttccag ggtccggagg atggcgtcgt tgacgtcttc caggatgtct
    13261 cgcagggcgc acccctgggc cgacgcgcca caaaagggca cctcgtgaca tcgggagatg
    13321 tccaaggggc cctccagaat ttccaccacg gccgccaggg tgatggcctc cgccggccgg
    13381 gccagggcgt accccccccg ggacccccgc atggaggaga tgagccctcc ccgtttcaac
    13441 tgtcccagga gctgctctaa ataggccacg gggagctgtc gtcgttccgc gatgtctttg
    13501 gccattacca aggttccctc cctggccagg gcgatgtcca tcatggcccg cagcccgtag
    13561 cgcgatcggg tggaaagttt caaagcgcac ccctccttgg aggctcaaag ccccgcagta
    13621 tgttcccctg tgacgtaact ttcgcaagga ttccaccccc ggggactcgt tctccggggc
    13681 ggcgaggatc ggcccgaaaa tccctgtgtt ccggtgtcgc tctggggctg gcggcgggat
    13741 gggggccttg gccctccgtc accggcggcg atgggtcggg ctgagggacc ctaggcggac
    13801 cggggggcga cgatctctcc tccccccgcc accacattcc cctcctctcc gtagagcacc
    13861 aggtgctgcc ccggtgcggg ggcgaagacc ggctcgtcga agcgacaccg aaggcggtcc
    13921 tctgtcagga gttccaccgt gccgggggtc agggggcccg gagcccggac cttaccccac
    13981 acccgggctc cgggaaagat cagatccggg gcgaggaggc ggggttgccg ggcctccacc
    14041 tcccgggcca gggccctctc ccggggcccc accaccaggg cctggcggag ggggtccacc
    14101 cggagcacgt agagggggac gggggaggcg atccccagcc cctggcgctg ccccggagtg
    14161 aagtggacga tgcccggatg gatccccagc tcgtgcccct cctcgtcgag gatggggccc
    14221 gggcggcgga gcagggcggg atccaggaca ctccggtatc cctccactcc ggcgaagcag
    14281 agatccatgc tgtcccggcg gagggcggtg ggaagccccg cccggcgagc ccgggcgcgg
    14341 acctcctcct tggagagctc tcccagggga aagagcgtcc gggggagtac ctccggggga
    14401 agggcgtaaa ggaaggcgct ctggtcccgg cgaaggtccc gggcccgggc gaggcgaacc
    14461 ccttccggcc ggtggaggat ccgggcgtag tgtcccgtgg cgatgaacga cacccccggc
    14521 aggctccgcg ccacctccca ggcgaggccg aacttgaggg cggcgttgca ccccacgcag
    14581 gggctggggg tctctccccg ggcgtagccc cggcggaagg gggataggac gcggtcttca
    14641 aagagctggc gggcatcgaa aaagacgtgg ggcatgccca gaaagcggca cacccccggc
    14701 gctgcgtcat ctcccgggga aaagagccgg agcgtcaccc ctaccccctc ccacccctcc
    14761 tcccggagga gccaggccgc gacgctggaa tccactccgc cgctcatgag cacggccacc
    14821 cggggtcggg ccggaagaaa tttttccatg gcacaccctt tcatggtcat ggggccgttc
    14881 tgcacgttcg gcagccccta caatgtgttc gcaagtttga tgcgatattg tcagacacaa
    14941 agcgctttcg ctgatgatat tgttcaacgt tttgaaaaat atgtgcttgt ccttcaattc
    15001 tatcacatgg ttttttatta gtgaatgatt taaccgatac cctaatccta cgcgtagggg
    15061 cgaaaaattt ttcgccccta caacattgtg taaatatgat cttcgggtgc cgtggcgctc
    15121 ctccgggcgg aggtggagtt ctagatctcc aggccgtcga agaggggcgt ggaaaggtat
    15181 cgctcccccg cgtcggggag gattaccacg aagacctttt cccggaactc ctcccgggcc
    15241 gcgaggcggg acgccaccgc cacggcggcg ccgctgctga tgcccgccag gatcccctct
    15301 ttccgggcca acgccctggc gaactccaga gcttcttcgg tgctcaccgt ctccgcccgg
    15361 tccaccaggg acaggtcgag ggtctcgggc aggaagcccg ccccgatccc ctggatgccg
    15421 tggggaccgg gatcgccggg gagccctgcc agatgccggg agatcaccgc gctgccttcg
    15481 ggctccaccg ccacggaaat gatggccttt ttctgggttt ttttgatgtg gcgagagatg
    15541 cccgtgaggg tgccccccgt gcccaccccg gccaccagca cgtccacggc gccgtcggtg
    15601 tcgcgccaga cctcggggcc cgtggtggcc tcgtggatgg cggggttggc ggggtttcga
    15661 aattgctggg gcaggaagaa gtggtccggg tcctccgccg ctagctcttc cacccggcgc
    15721 accgcccctg ccatgccctc tctttccggg gtgagcacga ggtgagcccc caatagggcc
    15781 agcaccttgc gccgctcgac gctcatgttt tccggcatga ccagggtcag gtcatagccc
    15841 cgggcggcgg ccacgtaggc cagggcgata cccgtgttgc cgctggtggg ttccacgatg
    15901 gttttttccg gggtgagaac tccccgggct tccgcatccc atatcagggc ggcccccacg
    15961 cggcatttca ccgaagaagt gggatttcgc ccctccacct tggccaggac cgtgactccc
    16021 ttccccgcca cccggtgaag tctcaccagg ggcgtgttac ccatggactg ggcatgatcg
    16081 tcaaaaatgc gggacatggc ggacgctcct ctccacaaga cggcgatggg ggccgttttt
    16141 ttgaagttga ctggtcccat aaacaatagg gttccctccc cccattgtca acgacgagag
    16201 acggaagggg ggcgcttttg tggtatcgtg gctcccgtgg ggcaaaaaaa gagaggcgtg
    16261 gccgattttc ggtgatggca acgatttttc ccgtgtgcat gggggaccct ggccccccga
    16321 acgctccagg gatttgaagg gaggagagcg tgctggaacg ctggatggca gggctggaaa
    16381 aagacgtcga gcggcgccgg gggtacgacg tggtgccctg ggccctatgc caggaggtgg
    16441 cccggaggga ggggatccct ttgggacagg tggaaaggat cgtcctgcgc cggggcctcg
    16501 tgccctcgcg ctacgagcgc aacgtgggca ccctggggtg cgaggggcag ctccgccttc
    16561 tggaggcccg gggggcggtg gtggggtgtg ggggattggg gggggtggtg gtggagctcc
    16621 tggcccgggc gggggtgggg accctggtgt tggtggatgg cgacaccttc gccgaccata
    16681 acctgaaccg ccagatccta tgccgggaag aggatctggg gcgtcccaag gtccgggtgg
    16741 cgatggagcg ggtgaggtcg gtaaatagcg cggtggaggt cgtcgccgtc gagaccttcc
    16801 tgacggaaga aaacgccccc gggatcctgg cggggtgcga tgtggcggtg gacgccctcg
    16861 atggccaatc ctcccgtcgg gtgctctttc gggcctgtgc cgccctggga ataccggcgg
    16921 tgcacggcgc catcggcggc ttctgggggc aggtgagcac cgtaatcccc ggagatcgct
    16981 ccgccctcga cctctggggc gtcgacgatc ccgaccgggg agcggaggaa gaaaggggaa
    17041 accctccctg cacccccgcc gccgtggggg ccctggaggc ggcggaggcc ctgaagatcc
    17101 tggcgggggt gggagagccc ctgcggggga agttgctctg gggggatctg gggtcccagg
    17161 agtggcgggt cctccccctg gggtgaggca ccccccgggg ccctttggtc aggccccgtc
    17221 gccgggggcg gtggcgcgag gagcttcggc gagggagcgg aaaaaccccc ctagggcctc
    17281 ctccagaagg ggagggtgga aggtcactcc cccgggcagg gatcgccgag accggaagat
    17341 gtcaaagagc atccctaggg ggaagtccca gggcgatccg ccggcggctt tgctccccag
    17401 gggttcggaa aactccccct cctcgacgcc ctcccagctt ccccgcagga ccagggaggg
    17461 gccgggcccc tcctccgggg cgtcggagga gggaccccgg aggaaaaggc tcagcgtccc
    17521 ggagcccagg ggtaggtcag acaggccagc gccgagctcc cggagggcgt cctccccggg
    17581 aaagagctcc cggcggagct cctcggagga aaaggaaagg gaccggcttt cccaggagag
    17641 ccctccgtct tcctcccagg tgccctctcg gaggagcatc ctccccgggg cgggttcggg
    17701 cccgaggcaa aagcggggat cggtccggca gatcgaggcc accaccaggg cctgggagag
    17761 caggggagcg aagtcctcgg cctcgaaggc gttcaggggc gcctcggggg cacaggggac
    17821 ctgggatcgg aagcggggcc acacgtcccc tccccggtag aagcgcttca aagacgtcat
    17881 cagggtccgg tcgtccttgt ccatggtctt tttcgtgccg aaggaaagga tcaggccgtc
    17941 ccgggtgaga ctccgctccg gcgcatgggg aggcattcct cccagggcta gcaggtggtg
    18001 acacaacccc cgagttcccc ggagggcgac aaatcccggc agagctcctc cgggccccgt
    18061 gaagcggcag tggtagatcc gatccgtacc ggggatgcgg atggcaaagg gagcgaagcc
    18121 ctccatccac ttccagggct cggtgcgccg catctcgtcg gccagggtga agaggtttct
    18181 ccactgcatg gcctcgggct ctttggggcg gtccgggggg gcggaggcgt agtgctgctt
    18241 cgtcgccttc ggagtttggg gcatgggggg tctcgcgttt tttctcatgg gtgctctcct
    18301 ctgggggctt tccccgggcg gacatacaaa aaagcaggga cccccggagc ccggagatcg
    18361 tcccccggag agcccttcac gtccgtttct cgatgcgctt ctgggagaga tgataccacg
    18421 aatgcccccg ttcccctcga agtaggggag aacttttgtc gcttcgcccc ggttttcccc
    18481 tagatccgga gttttgcgtc cgttttttcc tcacgccccc cgggagccga aggcggcggc
    18541 gtagaggtcg tggcgctcgt tggccaggag ggggcgtcgt cggcgcaggg cctccagggc
    18601 gtcccggtcg atctccacgt ccccttccgc ctcctccagg aggggaagct ccaaaagcac
    18661 ttccccttcg ggggagaggg cgaaggaccc gccgttgaac gccatggatc cctcccgccc
    18721 tccctgggtg gcgcagagga tgggggtggt gagggaaaag gccgtgccgc ggagcaagat
    18781 cttccactcc tccatgttgc cccttgcccg ggatgggtcg gggacgcagg gggccgaagg
    18841 cgccaggagg acttccatgc cccgggcggc gcacaccgcc gcgttcaccg ggtgccacag
    18901 gtcctcgcag atgaggatgc ccatgacgcc caggcgggtg gaaaccaccg tgaaagagtt
    18961 cccggggtcg aagtttcggt cctcggtgta gtgcccgtag ttcaccaggt tgagcttgcg
    19021 gtgaacggcc cgaagatgtc cccgctccac gtaggcggcg gcgatgaaga ttttgtcgtc
    19081 ctcgaagatc ggatagctca tgaggtagtc cacccccagc cgttccccct cggaggcgag
    19141 cccttccaga gcgtgggcca cgtcgcgggc cagggcggcg cggtgcgacg gcgagaggcg
    19201 tgccttctcc agcccgtacc ccgacaggtg catctcggga aagaccacca gcgaagcccc
    19261 tcgctccgca gcccgggcca gagcttcggc ggcgcgttgg gcgttgtgcg gcacgttcag
    19321 tagctccggg gccgtttggg ccaaggtggc acggaagatt agctccaaaa ttatccctcc
    19381 ttaaacagag ccccctcgtt tcgaaaaaaa tcctctgtcc cggggaaaaa tcgcctcggc
    19441 ggggacgaca ccccgccgag gcgaggatct caccgctggc gaaggggggc cccagaacag
    19501 gggccgaagg gcacacctca ggggcaagag ggggttccgg gggcgaaggg cgaggcatat
    19561 ccgccgggtc cccggaactc ccttccatca tgccctaccc tacttccccg gccccatcac
    19621 cggaaaagcc gggcgcgccg ggcgccgggg cgggtttttc tccagctcct ccagcaccac
    19681 ccgcagggcg gtttcgagct ggggatcccg cccggccagc accgccgccg ggtcttgctc
    19741 cacctcgatg tccggggaca ccccgacgtt ttccacgatc cagccgcgct cgtcccagag
    19801 ccccatgttg ggggccgtga cccaggtgcc atcctgtaaa aggggatagt ctgtgatccc
    19861 cacgagccct ccccaggtgc gcttgcccac caacacccct agccccttct tgcggaaggc
    19921 ccaggggagc atgtcgccgc cggatccggc ggactcgttg atgatcatgg ccttgggccc
    19981 ctccacgtag gccgcaggag agccgtagtc cgcccccgac cgcatggtga cccccgtggt
    20041 aaagcgccgg gagagggcgt cgaggacgta gtccgcgaac tgtcccccgc cgttgttacg
    20101 atcgtccagg atcagcccgt cccgggggag ctgggagaag aagtagcggt tgaagtattc
    20161 atatcccgcc gtggcggtgt tgggcatgta gacatagctc acccgcccgc cggagcgctc
    20221 ctccacccac cgcacgttcc cctccaccca ggccttgagg cgcagggcgc gctcggaatc
    20281 caggggaacc acggaaacca cccgggagtc cttccccgag ggatcggaag ccacggtgag
    20341 ctccaccaga cgattcgccg tgtgggcaaa gggggcgaag aagtcttcgc tggcggtgat
    20401 ctcccgatct cccaccttga ggacgaagtc cccctccttc acattcaccc cgggctccgt
    20461 gagaggagag cgcagcgagg gctcccagtt tacgccgccg aggatccggg tgaggcgata
    20521 gcgccctccc tccggggcca cgtccgcccc caggagaccg atctcctcct cttccccctc
    20581 ctgatcgggg aagtcccctc ccaccacgta gtggtgtccc acccggagct cgctcaacat
    20641 ggcggccaag agcccgtcga ggtcccggcg ccccgggagg tgatccagga agggaaggta
    20701 cttctcgtac accccgtccc agtccaggcc gtacatctgg gggtcgtaga agtagtcccg
    20761 gttgaggcgc caggcgtccc ggtagatctc cctccactcc tcccggggat tcaaggggat
    20821 gcgcacccgg cccaggtcca tggccttggc cgaggccagg tcccccgggg cgccgatggt
    20881 ccatcctccg tcgtgttgca ccaggagctt ttctcctcga agggagaccc gataatccca
    20941 aacgccccgc aggagcacct tttcctcccg ctcctccaag ttccaggagc agagcaggac
    21001 ggtacttccc ccgtcttccg tgccttccag ccgcaggtag tagatggtgc cctccttgcc
    21061 ggggcgcagg tcgctgtaaa atcccgccgg gatgggcagg gggagggtgc ggtccaagac
    21121 cccctcggcg tcgaaggaca ggggggcgtg ggccctatcc ttggcgtcgg gggatagagc
    21181 gggcgccggg ccttcgcccc cttggtcggc ggaggcccct tggtccgggg ccccttcctc
    21241 gccggaggcc tcctccagga cgccgggttc ttcctcgtcg ctctctcggg ccagggggga
    21301 ggggaccccc ttagccaaga tcgtcatgta gagggagcgg atgatgggct tgtcctgggt
    21361 ggacatgtcc agggtgaact tggcggggcc cgcgttggtg gagccgaaga agtacaagta
    21421 cttccccccc ggatcgaagg tggggcagat cacgtccgcc atgcggtccg acacggggaa
    21481 ggtctgcccc gtgtccacgc ggtggattac ggcctgctcc aggccgttgg tgagagtggt
    21541 ggcgaaggcc aggtggcagg agtcgggggc ccaggagagc tcaagccact tgtagggcga
    21601 accccaaagg ggctcgcgac acacctccac cggggccttc agtaaatcgg gggagagggg
    21661 acggtcgtcg gaggccgccg ccgcagcggc ggcgacgtcg atccagtaca ccgtggcgtt
    21721 ctgatcccgg aaggcgatct ttttcccgtc gggggaccag gcggggacaa agtacatccc
    21781 ccggcccagc aggggtacgg tgacctcctg cccccgggcg tcctggcggc ggatctttaa
    21841 gacgtactcc gggccgtcgt cggcgaacca agccaccagg cggccatcgg gggaccaggc
    21901 ggggtttcgg tcgtggacgc cggaagaagt ggtaagcgcc cgaggatcgc ccttatcggc
    21961 ggggacggtg acgatgtccc cccgcatggc gaagaccccc cgggcccccg agggggaaag
    22021 atcgaagccc tggatgaagt tttctccctt cacgaagcgt agccgagtct cggggaggtc
    22081 cgccgccagg gaaaggggga cgatttggta cccctccgag gcgggatctt tcaggcccaa
    22141 tctcccttcc cgcaggaaaa ggagcctccc ttccggggag gcggcgatgc tttccacggg
    22201 aaaggcgtca aaggccgtga gggctcgcac ctgtcgggtg gcggggtcga aggagtggat
    22261 gttaaactct cccccccggt ccgagaggaa gtagagggtg ttcccgatcc actggggctg
    22321 aacgtcgttg cacccctccg aaggctgggg cacggtggtg tcctttcggg tggcggggtc
    22381 aaagagatgg atcaccgaga tggtcccccc ccggtacccc ttccagatcc gaaaggccgg
    22441 gggcagagga ttgtaggcga agagccctcc gtcgggggaa aaggccccgt caaaggcctg
    22501 gggaagggaa agcctccgca ggggtccccc ctcccggggc acggcaaaca gggcgtagga
    22561 gcttcccagg gcggcctctc ggtccgaggt caagaggacc tccttcccat cggcggaaaa
    22621 ccccaggacg cgatccaccc cgggatgggc cgtgagacgc cggggcgtcc cccccgagac
    22681 gggcatgatg tacccatcca cgttgccgtg gttcattagg gagaagacca cgtccctgcc
    22741 gtcgggggag aaggcggggc gtcccaccgc cgtgccttcg gtgcaaaggc gccggaaggc
    22801 ggtgccgtcg gcctgggaaa cccagagatc cccggcgtag ataaagacca tttgggatcc
    22861 gaagagatcc cccgggccca ggagcaccgt ggccccttct tccgcccccg aagcccccag
    22921 gggagaaaaa agggccatca tcacggcgag gagtatgacc cccacgcccg cgctcccggg
    22981 accccgcatc ctcgagatcc cccttggaaa aatgccacat cgtaccgaaa ctctcatccc
    23041 tttttccctc ctttgggtga gttgagcccc tttgagacct ctggccggac cctaaagcct
    23101 tctaaagccc ccggagcatc gcccggggca aaacgacgac ggtccgtttc ccttccccga
    23161 ccgtcgaagc ccccttgaag caccccgcag aaccgaggag agagtacgct ccggagggcc
    23221 ttttagccag tcccgaagcc cctcggcccc gacggctatt ccggcagggc ccccgaagag
    23281 acgagggccg ccgcctttcg agcgatgcgc tgggcggccc gccccgaatc cgaaaggttt
    23341 gtgagcaccg ccacggcggt cttcgacgac gggtgggcca ggaagagggc cgaatacccc
    23401 gagatggctc cattcttggt gggcacgacc tgctcgtgcc aggtgaagac ctcgagcccc
    23461 atgccgaagg tggcctctcc gtcctccagg gggatggaaa aaacgcttcc gtcggaaagc
    23521 tccgccgggg agatcatggc ggcgatggtc tgctccgaga gcaacacccc ggga
//
LOCUS       DAOOER010000029        23442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_2523_length_23442_cov_7.976354, whole genome shotgun sequence.
ACCESSION   DAOOER010000029 DAOOER010000000
VERSION     DAOOER010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 23442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23442
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2523_length_23442_cov_7.976354"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1015)
                     /locus_tag="PL162_05395"
     CDS             complement(<1..1015)
                     /locus_tag="PL162_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQF91492.1"
                     /translation="MADLLASARGERPADLVLRGARVANVFTLEYEEVDVAVSGERIV
                     GVGPGYRGMREEDLSGLVLVPGFFDAHCHIESTMLTPAGFSELAVLRGTTAVGADPHE
                     IANTCGLAGVAYMWKEGLGCPVDMFFTAPSCVPASPFETSRETFDAAAVGEMFSRGWC
                     DSLGEVMNYPAVTGGDPSLWGILQAASGRVKSGHAPQLSGQELGAYLLSGCDSDHESV
                     TLEEGLEKLRRGMGIMIREGATEHNLEALAPLVRGRPALWDRCMMVSDDLTARYLMDR
                     GHMDEKIRLAGVQGIDPLGALRMVTLTPARYFGHRDRGGVAPGYLADLVAVGSLERCD
                     VRRV"
     gene            1273..2367
                     /locus_tag="PL162_05400"
     CDS             1273..2367
                     /locus_tag="PL162_05400"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HQF91493.1"
                     /translation="MAKKDGKNQPLPPNSPEPAFPSGDIDQRLARTFSARPIVIPDDG
                     RGPLLELINSSSRSILIKQFTFDDPELLGALLAARRRGVDVRVMLNARRSSGSRANDA
                     TFSTLKSNDVMVRWANPNYYVSHEKSMVVDSTWALVATFNFTSRTFESMRDYGVIIDD
                     IAAIADIVACFAADWRRAGFNPAPDTPLVWSKGNSRALMAHFIDRAKKDLYVQHPKFV
                     DATILDRLVLACRRGVEVRILCGGKSGISDFDMPDTFSSLRILKPFGAKIRRQKKTLR
                     LHAKLLIADKKVACLGSMNIDRSAFDLRRELGILLNGGAAVKRFSSLFDQDWDQGTPY
                     DPPDPVSVLPAPEEEDELTVRLGPDDDVEE"
     gene            2351..2938
                     /locus_tag="PL162_05405"
     CDS             2351..2938
                     /locus_tag="PL162_05405"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HQF91494.1"
                     /translation="MTWKNDLQGFDEPPAPPSLREIALAYLKIALSSFGGGLSAWARQ
                     IIVEEKRWLSDEAFLSALTFSRILPGANQVNLAVFVGTTLRGAPGALAALTGLLALPL
                     AIAVAFGMAYLSFSQNPLVSLVLSALTATSIGMTLATGFSISRRLPWHPATLLVAGAS
                     FVAIGPMKWPLLPVLVALIPLSVALHWRTAERKAP"
     gene            2938..3471
                     /locus_tag="PL162_05410"
     CDS             2938..3471
                     /locus_tag="PL162_05410"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HQF91495.1"
                     /translation="MALLGAIAQVFLPLSLMAFGGGNTIIAEIQRLVVDLHGWVTAEE
                     FSGMYAISQAIPGPSMMIVTLLGLAAGGLGGALVSTIATFGPCSALVYALARQWDRAK
                     DAPWRGAVERGLAPVAIGLIFAGGWALGREILDNPWEYAILALSTFVLARWNVNPLKL
                     MVAVGTLALGAGILLGG"
     gene            complement(3483..6545)
                     /locus_tag="PL162_05415"
     CDS             complement(3483..6545)
                     /locus_tag="PL162_05415"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQF91496.1"
                     /translation="MTEILLNAILHLFAIQSAMAPPSSRGLVREKVAAYLGDHLGLED
                     LRPYLDLFDMTLEMHGESPREDLRGHVWAVAEGLRSRLPRREQFVVLARFVEISGRCA
                     SGEGPESSAPGEEDRLDGDVARALGISETFVEDVRALCGDPLGEGARGENFLSLLPEG
                     VLPKAELGRSLLRPGFGGGFVVLRSEDPGVWFLRASEGEPTFLDNVPLAPSVLYQLKP
                     GSVLSNSRRDRIYFAEIAAAFVRPGRDRLIIFEGEHLEFRYPGSADVGLHDFSFSMEG
                     GQMGAIMGGSGAGKSTLLGVLTGCLPLKGGRLLINGLDVAEHAASLEGVVGYVPQDDL
                     LFDDLTVFENLYYSARLCLAHRSGEEIRLRCEELLSELNQSGTRDIKVGNPLEKTISG
                     GQRKRLNIALELIREPSILLLDEPTSGLSSADSRTVLGLLKAQAAKGRLVLAVVHQPS
                     GEIFKLFDRLWIFDEGGRPIYDGPPLGALLHFRTASYRAGSEEYACPHCGAVNPEQLF
                     EIIEEKCVDDDGFPTKERRVSAEEWHRLYLLSSPPLPERRERPSAPEEGRLERPSPLG
                     QFVLFFVRNFRSRAANRGYLMVNLWEPPLLALLAGALFRGAWGGEYVFRENTNLSGYF
                     FMSVVIAAFLGLSISSEEINKDRKILERERLLNLSWGAYTASKVLHLALVSAFQTGVF
                     VLLGHTILEIPDMYLLSWGVLWSTSCCTCMLGLNISAALKSTVAIYILIPILLVPQIM
                     LGGPTIPYDELIRKDAGNRLVPLVAEFMPTRWGYEALLVAHYTQNRFNVNFVDDDNVV
                     RWAEFLEGSYLPEVRGLASYPFLTPPAGEPKELRRQRVAQRLTALGHELRYLERYSGV
                     APALEDASLDVETYSRDVQRRVGGYLSRVEASIKALREESAQRRRATEDRMRATLGHQ
                     GFEELKNRHFNKEVAKLALGVALVDSVVLSGSRLVPQVLPIAWAPENRWGRAHFLAPF
                     KRLGPIVVATPLFDVGMLWVMALLLYIALWGRGLVRRGSLGRRGLRQR"
     gene            complement(6526..6648)
                     /locus_tag="PL162_05420"
     CDS             complement(6526..6648)
                     /locus_tag="PL162_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91497.1"
                     /translation="MSVEKTMMRQAPPILWGRLAFVDEGLFRGEGDIGRDGDPA"
     gene            complement(6639..7457)
                     /locus_tag="PL162_05425"
     CDS             complement(6639..7457)
                     /locus_tag="PL162_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91498.1"
                     /translation="MRSRWGLLALWVIGAIWMAGIPAAAAEKSKAEVLGAACEHLVEV
                     LNEKDLTALFGMMEKMKDAGLSWNKELTLPVSGIVACKTPEELYVLWGMYAMDMTYAH
                     LFGKGFEDIVAARVELEDRIGFPRDAFKKPVPESDREEKKNKSIEMIHEMIRQSQENP
                     VLLHTFVGGFYGSTLELFYLFCSLGLAAGVTDQVTDLINSHIPQLDLTYQILDAYEEN
                     GELAQLLDVGGRKAVIQSIMDVVKASLGNVREEDLQKVLSVVEPVRAPLKAPCQ"
     gene            complement(7810..7886)
                     /locus_tag="PL162_05430"
     tRNA            complement(7810..7886)
                     /locus_tag="PL162_05430"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7850..7852),aa:Arg,seq:ccg)
     gene            8234..8911
                     /locus_tag="PL162_05435"
     CDS             8234..8911
                     /locus_tag="PL162_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQF91499.1"
                     /translation="MKILVIEDNNALREIISEGLAGPENKVICANDGAEGLALAKRDP
                     FDCVILDLILPDQDGLDVLRSLRSQTMVPVLILSAKDKVEDRVAGLDQGADDYMVKPF
                     EMAELIARVRALVRRAANGGRALLGCGPLVMDTAARECRVHGELLTLRRREFDILELF
                     LKNENQVFTREQIINRVWKKEYEGGSNVVDVHIKYLRDRLRTSHLDRIIITVRGVGYK
                     LLCSEAE"
     gene            8930..9661
                     /locus_tag="PL162_05440"
     CDS             8930..9661
                     /locus_tag="PL162_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQF91500.1"
                     /translation="MDLSDSPPPLPTDRFGALMAHELRSALTLLEARIDTALLRPRSA
                     AYYEAALRQAKHHAGEVRGLLEALMDLDKLGRLPSPGKGSRCDLAKVAFAAGVPWEDA
                     HRPGETTIRWELRPAPVWGDPRLLGRMMINFLDNAVKYSSPGSPIAVYTGREGEKSFL
                     QVTDRGVGMTEEEIRQCLEPFWRSADCQDRASGYGLGLPLVCRIVRLHGGDMEIMSRK
                     GEGTTVRASFPSAGGDPELEDRGEP"
     gene            complement(9843..11231)
                     /locus_tag="PL162_05445"
     CDS             complement(9843..11231)
                     /locus_tag="PL162_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HQF91501.1"
                     /translation="MDRSGKWKVFVGVMVLGAILLGALSDARAQDVYTGNPVVKIARE
                     AFPAVVNIDTEKMVTRTPFPFGDDPFFREFFGDQGRRFSDIVPMRGAGSGFLVTADGY
                     ILTNNHVVAEADKITVTLASGKEYPAKLVGRDPTFDLAVVKIDAKDLPVLSLGDSDKL
                     EVGEWVAAIGNPFGLEHTITVGVISAKNRNIRAKDVNFQGFLQTDAAINPGNSGGPLL
                     DLSGKVVGINTAIIPYAQGIGFAIPVNMAKQVMDDLVNFGKVRRGWLGIYLQPVTEEV
                     AEAYGLKNTDGALVSDVQGDSPALRAGLQRGDVIVEIDGTSLKNYQDLSFAVRKHLAG
                     DEVTLTVMRRGERKEIKVRLGELPGQVTGESPEGNAVEEKLGLAVAPVDGARRTKYNL
                     GDHKGMVVTEVKRGTPAERLGIREGDLVLEANGVPVPDEAAWNKALGKAHKGVVLLIL
                     RDGRTFFISLRM"
     gene            complement(11355..12146)
                     /locus_tag="PL162_05450"
     CDS             complement(11355..12146)
                     /locus_tag="PL162_05450"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HQF91502.1"
                     /translation="MYPILFSWGGVPVHSYYILWTFSLMLAVFWTKRRLMGPFALDED
                     RAVTILLWGLVGLYVGARLGAVYDHWDYFSSHPWRIVSPWEGGLSAITAFAGAGIGAA
                     WRCHRLKVPLETVAEAASLPAAVTETLGRWGCFLNGCCYGVETTPPWGMHFPFDPEGV
                     VRHPTQIYYALGELGLLLLLMGVERWIGKGSERRVRGAVLWPLFMVGYALLRWSVDPW
                     RHENVPTIHLMGFFLTGTAALAGLVWLGATWSDFRKCRKEGSSRP"
     gene            complement(12152..12820)
                     /locus_tag="PL162_05455"
     CDS             complement(12152..12820)
                     /locus_tag="PL162_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HQF91503.1"
                     /translation="MAEQDMVVFEAASMEEARQIASERLGLAPEDFMVTVREEGRRFF
                     GLLGKKNTYQAAPVAPRHLLRVREVVRQMARRMGVDVDVVFGEEDGVDLRGTDREMLL
                     GTYGEPLRALEYLVNIMLREPLEGRWVRFDSEGFREHRQAHLEEAARQAASEAVRRRR
                     PISLDPMTSWERRVVHVALKDNAEVETRSVGEEPTRRVVVWPQKNVARASTGKMVYRT
                     RLPR"
     gene            complement(12887..13687)
                     /locus_tag="PL162_05460"
     CDS             complement(12887..13687)
                     /locus_tag="PL162_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HQF91504.1"
                     /translation="MSALWSAGENLLLQILVLFYNLTTSYGVAIILLTVLVRILLYPL
                     SQKQMVSMQKMQKLQPRAKVLQEKYANDKETMNKEIMLLYKEHKVNPAAGCLPILVQL
                     PILILLFNVLNNQEKLTTVLHLTKESFSFLGIHLHSSLYAVLGQALGIGSSPDVGIMA
                     ILSAMGTNIAGLAQVGLYGANALLVVVIAFLTWFQTQLSASSNPQMGNMNWFMTLFLT
                     FICLGMPGGVLLYWVVSSAIGVGQQWWVYHRTEREMLEKPVLHKDKPR"
     gene            complement(13819..14010)
                     /gene="yidD"
                     /locus_tag="PL162_05465"
                     /pseudo
     CDS             complement(13819..14010)
                     /gene="yidD"
                     /locus_tag="PL162_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
     gene            complement(14061..14474)
                     /gene="rnpA"
                     /locus_tag="PL162_05470"
     CDS             complement(14061..14474)
                     /gene="rnpA"
                     /locus_tag="PL162_05470"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HQF91505.1"
                     /translation="MRFSYPPSARLRSGWEFDLLFRTGRRAQGRLVRLLFLDRGEGGA
                     RFGLVVGKRQGGAVVRNHGRRLLREAVRHVRPLVRDGLWIVVSLKNDALRAPVPEIFD
                     DLRSLLGRGGLLREEVPRYRWDDVVPKSVPVGGSP"
     gene            complement(14481..14615)
                     /gene="rpmH"
                     /locus_tag="PL162_05475"
     CDS             complement(14481..14615)
                     /gene="rpmH"
                     /locus_tag="PL162_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012082253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HQF91506.1"
                     /translation="MKRTFQPHKKPRKRSMGFLVRSSSPAGRRVLRNRRRKGRARLAV
                     "
     gene            complement(14946..16892)
                     /locus_tag="PL162_05480"
     CDS             complement(14946..16892)
                     /locus_tag="PL162_05480"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HQF91507.1"
                     /translation="MMEMYALFGPEGPFARAFDGYEVRPPQQQMAERVRHLCEEAPPG
                     WLLAVEAPTGVGKSFALLAPALLWATQEEGRVLLLTASIPLQEQLLRKDIPRLLQVLG
                     LPVSYTLLKGRSHYLCRRRALEMTTEGSLSFGDRGEGTRKLGAWMERTATGDLDELSL
                     PPGHPALLAAAASASSCAGRACPHREECAVTAALRRAQGSTLVVANYHLFFSYTLGLG
                     TPFPVPFDVLLCDEAHRMAEAARSSSSRGTSQEEWQRLLGARVVSSPGDALEKLGVDL
                     SGASEDLVGCRREVEAFFGQMRIRWGEGHTWRRAEEGVGVDGGPLIALADQLVKRFAP
                     LAERDPELIPEEGHEILSWSKALRKMREDLAWCTAVDHFPSWAYWWDGRQLCSTPVEC
                     ADLVGESMEKAPCRGVVAASATLALGGSVENWSTETGLTPNEVLVLDSPFPLAQQMEL
                     WVVRMGLRVQDPGYDALVARVVERLVKDNGGRSLVLLSSMRLLRVVGERLKSTPRPFK
                     VLVQGDLPRGELLEFFRQDETSVLVGSVSFREGVDIPGNGLTQVIIDRIPFPHPKDPL
                     VEARQELLGRKAFSSSALPWAKLLLKQAVGRLIRSSSDRGRAVILDCRVTDYPEWEIL
                     ESLPRVPVRRMNLRVPENEEMGSP"
     gene            17050..18030
                     /locus_tag="PL162_05485"
     CDS             17050..18030
                     /locus_tag="PL162_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HQF91508.1"
                     /translation="MRRLAVPLLLIALGGSIVGGHLPEDGRGSSPAEAFFLFEEEDPS
                     APPEPFTWRRALPLDDVARLFREGGFLALCEGGSSSDVAAALLRGADPNLSNDRGRSA
                     LMFALANPDPAVAQILLEGGADPRAVDREGHPVWIFLPPGGALRPLALLLDFGLNPRS
                     PGASGVEMPFILAAAEDAPATLPLLLDRGVPVDLRDSAGATLLMWAATNPRPGGVEAL
                     LLRGADSRAVDGEGRTPLHYALESPYPGGEVVRALLRGGADPNARTVEGTTPLALAAR
                     QGAPREVFEALLRGGALPFLGDEEGLTPCDEARTNPDLRHRRDILKLLCP"
     gene            complement(18056..19099)
                     /locus_tag="PL162_05490"
     CDS             complement(18056..19099)
                     /locus_tag="PL162_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HQF91509.1"
                     /translation="MKRSSAHYRYHVLKILGIGMVLTFLGAGVGTYFFFWHPRDPGLR
                     GTSPTPGPSVRRENPKASLSPGAVAAVDLDRLFREAGARSAEDPLMDHPVPGTTEVPL
                     RISFEPREVRRAQGEGFSYVFRVKDAGRELLQYEFSGDGSFAGTFVGMGRSALRHLLG
                     ETPESLGPFDFFPQGTGDDRGVLCLYDGGGDDASLRALAFGETQLGSFGPRFLEARRE
                     ARGVPGALTGTKVNVRQEPTTKSRVVTNLAGNESLTVYQSSPEYYDARLLRAVSVFCL
                     GKKYDLRKGTSVRVVGKEQEGMVPVVFRRDLSAYVERDAVGTGPDALWHYVRTPSGHE
                     GWVRGDFISTVRP"
     gene            complement(19308..20264)
                     /gene="arcC"
                     /locus_tag="PL162_05495"
     CDS             complement(19308..20264)
                     /gene="arcC"
                     /locus_tag="PL162_05495"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HQF91510.1"
                     /translation="MSTTKVVVALGGNALQEAGTPATAEAQLDVVRRTVEYLAELSCR
                     GYEMAVVHGNGPQVGRIVLASETAAKVNPETPAMPFDVCGAMSQGYIGYQIQQALRDA
                     LRRRNRNVPVVTLATQVVVDAKDEAFRNPTKPIGAFYSEEEAQRIAEEKGYNMKEDAG
                     RGWRRVVPSPAPVRIVEIDAVKRLWDTTIVVTCGGGGIPVVEERDGSLRGVAAVIDKD
                     LAAERLAEDIQADVLLILTEVDRVAINFKKPDQRDLRRMTVPEAIQYVDEGHFAPGSM
                     LPKVKAAVKFARTFPGKKAIITSLYKAVEALEGQEGTEVVMA"
     gene            complement(20630..21724)
                     /locus_tag="PL162_05500"
     CDS             complement(20630..21724)
                     /locus_tag="PL162_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91511.1"
                     /translation="MRKRFLWGAFAAVALLLGSGAATAALRLGPLPPGWQERAAPAPG
                     VASYYVLQVGKSVWAEAFFFTEELAAPMDVRSYFQAFQANSLMTGSFRNYAAGEVGDA
                     TVGGRPAVRHDFTFTGQGEGAAELKARLFLFMDGKTARAILFDGLRKDFPALEAQFAN
                     FLQAVFPEGTGTSKPPLFPGQGATGSPGPSVVPLTPPPPVTGVGAISSGDDLPALSPD
                     LSEGELPDLTEPPDPGLFVDASKKIRVRLPDASVPQEVTSSKGVYSGPDMSVISVLVL
                     AGEQEASAEATRRGAGQTFFGVNTLKNAVGKICRAGLYAAPEASGQRKATMIATYPGT
                     PLLVEVVLPAERYDGAKGWIKTLLAGVELP"
     gene            complement(21950..22399)
                     /locus_tag="PL162_05505"
     CDS             complement(21950..22399)
                     /locus_tag="PL162_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HQF91512.1"
                     /translation="MKKLMKKQRGFTLVELLIVMIIIGILAGGMMMVMGSSTDKADAT
                     KALSDLRTMASSTMQYYADNGAQAALPTVADLSRMMNKTLGGSYDVAGSGDQAWVGVY
                     VPTAGSRARLADIRAQEGLVIVGSATKGTVGSVDFTAAHVWAYMRAK"
     gene            complement(22532..>23442)
                     /locus_tag="PL162_05510"
     CDS             complement(22532..>23442)
                     /locus_tag="PL162_05510"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HQF91513.1"
                     /translation="YAVVQVKKMLDNGVSLAAAMKNRPEFNTLMVAMVQAGEEGGLLD
                     VTVNRLASFLEKQETLQRKIKGALTYPTVIMTFATLVLYLMVTVIVPKFKGALDNLNT
                     ELPWITTVIFDLADFFGNKWYIILGAIVALVAIWVVLGSSKATRPFVDRVRLRVPVFG
                     DLQYKAIMARSTRTLGSLVQAGVPILSCLEMAGEVSDNYEVRQGFAMLREAAKRGQGL
                     GETAKGVKIFPLMVAHMIAIGEQTGRLEEMMNKVADWFEAELDVKVGQLNSLLEPMLI
                     VFVGGVVAVIAMAIFFPIIGAIQTML"
BASE COUNT         4333 a         8078 c         6887 g         4144 t
ORIGIN      
        1 aaacccgccg cacgtcgcag cgctccaaag accccaccgc caccagatcc gccaggtatc
       61 ccggcgccac gcccccccga tctcggtggc caaaatagcg ggcgggcgtg agggtaacca
      121 tgcggagggc ccccagggga tcgatcccct ggaccccggc gaggcgtatt ttctcgtcca
      181 tgtgaccccg gtccatcaaa tagcgcgccg tcaggtcgtc gctcaccatc atgcaccgat
      241 cccagagggc ggggcgcccc ctcaccaggg gggccaaggc ttcgaggtta tgctccgtgg
      301 ccccttcccg gatcatgatc cccatgcccc ggcggagctt ttccagcccc tcttccaagg
      361 ttacgctctc gtggtccgag tcgcacccgc tcagcaggta ggcaccgagc tcttggccgg
      421 acaactgagg ggcatgaccc gatttaaccc gccccgacgc ggcctggagg ataccccaca
      481 gagaggggtc tccccccgtc acggcggggt agttcatcac ctcccccaga gaatcgcacc
      541 acccccgaga gaacatctct cccaccgccg ctgcgtcaaa ggtctcccgg gacgtctcga
      601 agggggaggc ggggacacag gaaggcgccg tgaaaaacat atccacgggg caccccagcc
      661 cctctttcca catgtaggcc accccggcca ggccgcaggt gttggcgatc tcgtggggat
      721 cggcccccac cgccgtggta ccccgaagca ccgccagctc cgaaaagccc gccggagtga
      781 gcatggtgct ttcgatgtgg cagtgagcat cgaaaaaacc cggaacgagc accaagccgg
      841 agaggtcttc ctcccgcatc ccccggtatc ccgggcccac ccccacgatc cgctcccccg
      901 acaccgccac gtccacctct tcgtactcca gggtgaagac gttggccact cgcgctcccc
      961 gaagcaccag gtcggcgggg cgctctcccc gggccgacgc cagaagatct gccatggcca
     1021 cctccgatcc cctcctttct cctcttcact ttttactgtg ggggccttcc cgcccccctc
     1081 accttccggt cccgcgcccc gagccccccg atctttgaca ggatcccccc gaaacggcat
     1141 aatctccggg gaaagatcgc ccttcggccg ccccttcccg ccccgacgga agctcccctt
     1201 cgggccgagg tgcggctctc tcttcgagtc gcctccggcg acccgaaaga acccctcgct
     1261 ggaggtgcat ttatggcaaa aaaagacgga aagaaccaac cacttccccc gaattcgcca
     1321 gagcccgcct tcccctcggg ggacatcgac cagcgcctgg cccgaacctt ttcggcgagg
     1381 cccatcgtga tccccgacga cggccgaggg cccctgttgg agttgatcaa ctcctcctcc
     1441 cggtccatcc tcataaagca gtttaccttc gatgacccgg agctgctagg ggccctcctg
     1501 gcggcccggc gccggggagt cgacgtccgc gtcatgctga acgcccgccg ctcttcgggg
     1561 tctcgggcca atgacgccac cttctcgacc ctgaagtcaa acgacgtgat ggtccgttgg
     1621 gccaacccca actactacgt atcccacgaa aaatccatgg tggtggattc cacctgggcc
     1681 ctggtggcca cattcaattt cacctccagg acctttgaga gcatgcggga ctacggggta
     1741 atcatcgacg acatcgccgc catcgcggac atcgtggcct gtttcgccgc cgactggcgc
     1801 cgggcgggat tcaaccccgc ccccgacacg ccgctggtct ggagcaaggg gaactcccgg
     1861 gccctcatgg ctcacttcat cgatcgagcc aagaaggatc tctacgttca gcaccccaag
     1921 ttcgtcgatg ccacgatctt ggaccgactg gtcctcgcct gtcgccgggg ggtggaggtg
     1981 cgcatcctct gcgggggcaa gtccgggatc tccgatttcg acatgcccga caccttctcc
     2041 tccctccgga tcctcaagcc cttcggggcc aagatccgac gccagaaaaa aactttgcga
     2101 ctgcacgcca agctcctgat cgcggacaaa aaggtcgcct gcctgggatc gatgaacatc
     2161 gaccgtagcg ccttcgacct gcgccgagaa ttgggcatcc tcctaaatgg cggcgcggcg
     2221 gtgaaacgct tttcaagcct cttcgaccag gactgggacc aggggactcc ctacgacccc
     2281 cccgaccccg tctccgtact tccggcgccg gaggaagagg acgagttgac ggtcagattg
     2341 gggcccgacg atgacgtgga agaatgacct ccagggcttc gacgaacccc ccgcgccccc
     2401 ttccctccgg gaaatcgccc tggcgtacct aaaaatagcc ctatcgtcct tcggaggagg
     2461 gctctcggcc tgggcccgcc agatcatcgt ggaagaaaag cgatggcttt ccgacgaggc
     2521 cttcctgtcg gcgctcacct tcagccgcat cctccccggg gccaaccagg tcaatctggc
     2581 cgtcttcgtc ggcaccacgc tccggggcgc cccgggggcc ttggcggccc ttacgggcct
     2641 tttggccctc cccttggcca tcgctgtggc cttcggcatg gcctatctct ccttctccca
     2701 aaaccccctg gtgagcctgg tcctttcggc gctgacggcc acctccatcg gcatgaccct
     2761 ggccacggga ttctccatat cccgacgcct cccctggcat ccggccaccc tcctcgtggc
     2821 tggggcgtcc ttcgtcgcca tcggccccat gaaatggccc ctgctgccgg tcctggtggc
     2881 gctaatcccc ctttccgtgg cgctccactg gcgaacggcc gaaaggaaag ccccctgatg
     2941 gctctcctcg gggccatcgc ccaggtcttt ctccccctgt cgctcatggc cttcgggggg
     3001 gggaacacca tcatcgcgga aatacaacgc ctcgtggtgg atctgcacgg ctgggtaacg
     3061 gcggaggagt tcagcggcat gtacgccata tcccaagcca ttccaggccc cagcatgatg
     3121 atcgtcaccc tgctgggcct tgcggcggga gggctgggag gagccctggt ctcgaccatc
     3181 gccaccttcg gcccctgctc cgccctggtg tacgccctcg ctcggcaatg ggaccgggca
     3241 aaagacgccc cctggcgcgg cgccgtcgag cggggcctcg cccctgtggc catcggcctc
     3301 attttcgccg ggggatgggc cctggggcgc gagatcctgg ataacccttg ggagtatgcc
     3361 atcctggccc tctccacctt cgtgctggct cggtggaatg tcaatcccct taagctcatg
     3421 gtcgccgtgg ggaccttggc cctgggggcg gggatccttt tggggggatg acggcagggc
     3481 ccctacctct gcctgagtcc ccttctcccc agggatcccc gccggaccag ccccctaccc
     3541 cagagggcga tgtagagcag aagggccatg acccacagca tccccacgtc aaagagggga
     3601 gtcgccacga cgatgggacc caggcgcttg aagggggcca aaaaatgcgc ccttccccat
     3661 cggttttcgg gggcccaggc gatgggaagg acctggggta ccaatcgact ccccgaaagc
     3721 accacgctgt ccaccagagc cacccccagg gccaacttcg ccacttcctt gttgaagtgg
     3781 cgatttttta gctcttcgaa cccctggtgc cccagggtgg cgcgcatccg atcctccgtg
     3841 gcacgcctcc gctgggccga ttcctcccgg agggccttta tggaggcctc cacccggctc
     3901 agatagcccc ccacccgccg ctggacatcc ctggaatagg tctcgacgtc gaggctggcg
     3961 tcttccaggg ctggggcgac gcccgagtag cgttccaggt agcggagctc gtggcccaag
     4021 gccgtgagcc tctgggccac tcgctggcgc cggagctcct tcggctcgcc cgccgggggg
     4081 gtcaagaagg gatagctcgc cagccctcgg acctcgggca gatagcttcc ctcgaggaat
     4141 tcggcccacc ggacgacgtt gtcgtcgtcg acaaagttga cgttaaagcg attctgggtg
     4201 taatgcgcca ccaagagggc ctcatacccc cagcgcgtgg gcatgaactc cgccaccagg
     4261 gggacgaggc ggttccctgc gtccttgcgg atgagttcgt cgtaggggat ggtgggcccc
     4321 cccagcatga tctgaggcac gaggaggatg gggatgagga tgtaaatcgc cacagtggac
     4381 ttgagggccg ccgaaatgtt gaggccgagc atacaggtgc agcagctcgt gctccagagc
     4441 accccccagg aaaggagata catgtcgggg atctccagga tcgtatgccc cagcaacacg
     4501 aagacccccg tctgaaaggc cgaaacgagg gccaggtgga gcaccttgga ggcggtatag
     4561 gccccccagc tcaggttcaa aaggcgctcc cgctccagaa tcttccggtc tttatttatc
     4621 tcttccgagc tgatggaaag ccccaggaag gcggcgatga ctacgctcat gaaaaaataa
     4681 cctgaaagat tcgtgttttc tcggaaaaca tactctcccc cccaggcccc gcgaaagagc
     4741 gcccccgcga ggagggccaa gagggggggt tcccacaggt tcaccatgag gtatcccctg
     4801 ttggccgccc ggctcctgaa gttcctgacg aaaaacagga caaactgccc gaggggagat
     4861 gggcgctcca gacggccctc ctcgggagcc gaaggccttt cccggcgctc cgggagggga
     4921 ggggacgaca gaaggtacaa gcggtgccac tcttcggcgg agacccgacg ttccttcgtg
     4981 gggaatccgt cgtcgtcgac gcatttttct tcgatgatct caaagagctg ctcggggttc
     5041 accgccccgc agtgcggaca ggcgtactct tcgcttcccg ctcgatagga ggccgtacgg
     5101 aagtgaagca gggcacccaa tggggggccg tcgtagatgg ggcgcccccc ctcgtcgaag
     5161 atccatagcc gatcaaacag cttaaagatc tcgccggaag gctggtggac caccgctaaa
     5221 acgaggcgtc ccttggcggc ctgggccttg aggagcccca ggacggtccg ggagtccgcc
     5281 gaggaaaggc cactcgtggg ctcatccaaa aggaggatgg aaggttcgcg aatgagctcc
     5341 agggcgatgt tcaggcgctt ccgctgaccc ccgctgatgg ttttttccag gggattgccc
     5401 accttgatgt cccgcgtgcc gctctggttt agctccgaaa ggagctcctc gcaccttagg
     5461 cggatctctt caccgctccg gtgggccaga cagagacgag cgctgtagta gaggttttcg
     5521 aacaccgtca ggtcgtcgaa gagaagatcg tcctggggga cgtaacccac gaccccctcc
     5581 agcgaggcgg cgtgctcggc gacgtcgagg ccgtttatga ggagccgacc ccctttcagg
     5641 ggaaggcatc cggtgaggac ccccaacagc gtcgactttc cggcgccgct gccccccatg
     5701 atcgccccca tctgccctcc ctccatggag aagctgaaat cgtgtaaccc cacgtcggcg
     5761 ctgccgggat aacggaattc caagtgctct ccctcgaaga tgatgaggcg gtctcgccca
     5821 ggcctgacaa acgccgccgc aatctcggcg aagtaaatcc ggtccctccg ggaattggaa
     5881 aggacggacc ctggtttcag ctggtacagg acggacggcg ccagggggac gttgtcgagg
     5941 aaggtcggct ctccctccga cgcgcggagg aaccagaccc ccgggtcttc ggagcgcaac
     6001 accacgaagc cccctccgaa tcccgggcgc aataggcttc gaccgagctc cgccttcgga
     6061 agcaccccct cggggaggag agacaggaag ttctcccccc gagccccctc gccaagggga
     6121 tcgccgcaaa gggcgcgcac gtcctccaca aaggtttccg agatacccag ggcccgcgcc
     6181 acatcgccat ccaagcgatc ctcttccccg ggggccgagc tttcggggcc ctcccccgag
     6241 gcgcagcgcc ccgagatctc cacgaagcgc gccaacacta cgaactgctc ccgacggggc
     6301 aggcgcgaac gcagcccttc ggccaccgcc cagacatgcc cccggagatc ctcccggggg
     6361 ctctctccgt gcatctccaa cgtcatgtca aagagatcta ggtaggggcg aagatcttcc
     6421 aaccccaggt gatcgccgag ataggccgcc accttctctc ggacgagccc ccgggaggac
     6481 ggcggtgcca tggccgactg aatggcaaat agatgtaata tggcgttaag caggatctcc
     6541 gtcacggccg atgtcccctt ctccccggaa tagcccttcg tcgacaaagg ccagacgccc
     6601 ccacaggatc gggggcgcct ggcgcatcat cgtcttttct actgacacgg agccttcagc
     6661 ggggcacgca ccggttcgac aacggaaagg accttttgga gatcttcttc tctcacattt
     6721 cccagagagg ccttcaccac gtccatgatg gactgaatca ccgccttgcg accgcccaca
     6781 tcgagcaatt gggccaattc accattttct tcgtaggcat cgaggatctg gtaggtaagg
     6841 tcgagctggg ggatgtggga gttgatcagg tccgtcacct gatccgtgac ccccgccgca
     6901 agacccaggg agcagaacag gtaaaagagc tccagggtgc tgccataaaa ccctcccaca
     6961 aaggtatgga gcaggacggg gttttcctgg gactggcgga tcatctcgtg gatcatctcg
     7021 atggatttgt ttttcttttc ctcgcggtcc gactctggaa cgggcttttt gaaggcgtcc
     7081 cggggaaagc cgatgcggtc ttccagctcc acccgcgcgg cgacgatgtc ttcgaagcct
     7141 ttcccgaaca ggtgggcata ggtcatgtcc atggcgtaca tcccccacag gacgtaaagt
     7201 tcctcggggg tcttgcaggc cacgatcccc gatacgggga gcgtgagctc cttgttccac
     7261 gacaagcccg catctttcat cttttccatc atgccgaaaa gtgccgtcag gtccttctcg
     7321 ttcaagacct ctacaaggtg ctcgcaggcg gctcccagca cctcagcctt gctcttttcg
     7381 gccgccgccg ccgggatccc cgccatccat atcgcgccga tcacccacag agccaaaagt
     7441 ccccacctag atctcacacc acttaccccc ttttctcttg ctctccgaag cgctgtttcc
     7501 gccgcccccc aagacttata tcatttccct ttccgcgaca gccacaaagg cccataaaat
     7561 ctccgccgaa gctcccctct tattgccagc cccgaccgaa gcaagcgccc ggaatttctg
     7621 gctaattttt ttcacccaat atcgccctgg ccctttcgtg ccccgcattt tcaggaaaaa
     7681 ccccaggccc ggcgaggaac gcaaaaaccc ccttttcacc agaccgagga gttgtctgcg
     7741 aaagggggct tttcacatca tttgggcttt tgcacgaccg ccgccgcaaa aaccctgtgg
     7801 acaacaaaat ggcgcgcccg gcgggattcg aacccacgac ctttggctcc ggaggccaac
     7861 actctatcca gctgagctac aggcgcctat tccaaatctt cgacagggag taggatactc
     7921 tccgggcctc gtcttgtcaa gctgcccgtc cggcggtggg acttgccggg ccccgagccc
     7981 tgacgggggc gataacgcct ccctttccct cccttctggc ccctcgagcc cccataaaaa
     8041 ggggcaaggg caagaggaca atttttaatt aaaaaatatc cattttaaaa cattttctgt
     8101 gaaaaaatat tctcaaaatc attttttccc ctccaccccc tcgacaaggg gaaatctttg
     8161 tgttctaata tatgtcgttc cgacacgatc gtcgggcgca ttcgattcga agaatcggga
     8221 ggggcgaaga cacatgaaga ttttggttat cgaagacaac aacgccttga gggagatcat
     8281 ttccgaaggc ctcgctggcc cagagaataa ggtcatctgt gcaaacgacg gagccgaggg
     8341 gttggcgctg gcgaagcgcg atccctttga ctgtgtgatc ttggacctta tcttgccgga
     8401 tcaggatgga ttggacgtgc tgcggtccct tcggagccag accatggtcc ccgtgctgat
     8461 cttgagcgct aaggacaaag tggaagatcg tgtggcgggg ctcgaccaag gagcggatga
     8521 ctacatggtc aaacccttcg agatggccga gctgatcgcc cgggtccggg ccctggtgcg
     8581 ccgggcggcc aacggcggtc gggctctgct gggatgcggc cccctcgtca tggacaccgc
     8641 cgcccgggag tgccgcgtcc acggggagct cttgacgctc cgtcgtcggg agtttgacat
     8701 ccttgagctg ttcctgaaga acgagaacca ggtctttacc cgggaacaga tcatcaatcg
     8761 ggtctggaaa aaggagtacg aggggggtag caacgtggtg gacgtccaca tcaagtacct
     8821 ccgcgaccgc ctacgcacct ctcaccttga ccggatcatc atcaccgtgc gcggcgtggg
     8881 atacaagctt ctctgctcgg aagcagagta aggtcggtct cgaggggcca tggacctctc
     8941 cgactcccct ccaccccttc cgacggaccg attcggggcc ctgatggccc acgagcttcg
     9001 gtcagctctg acgttgctgg aggcccgcat cgatacggct ctcctccgtc cccgtagcgc
     9061 cgcctactat gaggcggcgc tacggcaagc caaacaccat gcgggggagg tacgggggct
     9121 tctggaggcc ctgatggatc tggataaact gggacgtctg ccctcccccg gcaagggatc
     9181 ccggtgcgat ctggccaagg tcgccttcgc ggcgggcgtc ccgtgggagg atgcgcaccg
     9241 ccccggcgaa actacgatcc gctgggaact tcgccccgcc cccgtgtggg gggacccccg
     9301 cctcctgggg agaatgatga ttaattttct cgataacgcc gtgaagtact cttccccggg
     9361 atctcccatt gcggtgtata cgggaaggga aggggaaaaa tccttcctgc aggtgaccga
     9421 ccggggggtg ggcatgacgg aagaagaaat ccgccagtgc ctagagccct tctggcgatc
     9481 ggcggattgc caggaccggg cctcgggcta cggcctgggg cttcctttgg tgtgccgcat
     9541 cgtccgactt catgggggcg acatggaaat catgtcccgc aagggcgaag gaaccacggt
     9601 acgggcctcc ttcccctccg ccggtggtga tccggagctt gaggatcggg gggagccgtg
     9661 acatcgatcc atcctacatc ggaaccatat tttgccgttc ccagaacaaa gtcgtccccg
     9721 ggggcggctt ttgccatcaa ggcggggggg gcagaggccc atgacatccc cccgcccccc
     9781 tatttcccta tttcggcccc ccgggttagc ccggccccat ccccacgccc cctccgacgc
     9841 ctctacatcc tcagcgagat gaaaaacgtc cgtccatcgc ggaggatcag gagcaccacg
     9901 cccttatggg ctttgcccag ggccttgttc caagcggctt cgtcggggac cggcacgccg
     9961 ttggcttcca gcaccaagtc cccttcgcgg attcccaggc gctccgccgg ggtgccgcgc
    10021 ttcacttcgg tcaccaccat gcccttgtgg tctcccaggt tatacttggt ccgccgggcc
    10081 ccatccaccg gcgccacagc caagcccaac ttctcttcca ctgcgttccc ctccggggac
    10141 tcccccgtca cctggccagg caattcgccg aggcgcacct tgatctcttt tctctctccc
    10201 cggcgcatca ccgtcagggt cacctcatcc cccgccaggt gcttgcgcac cgcaaaggag
    10261 aggtcctgat agttcttaag ggaagtgccg tcgatttcca cgatgacgtc ccctcgctgg
    10321 agtcccgccc gcagggcggg ggaatctccc tggacatcgc tcaccagagc tccgtcggtg
    10381 ttttttaggc cataggcttc ggcgacttcc tccgtgacgg gctggaggta gatgcccagc
    10441 catccccgcc gcaccttgcc gaagttaacc agatcatcca tcacctgttt cgccatgttc
    10501 accggaatgg cgaagccgat gccctgggcg taggggatga tggcggtgtt gatccccacc
    10561 accttgccgg aaaggtccag caaggggccc ccgctgttgc cgggattgat ggcggcgtcg
    10621 gtctggagaa atccctggaa gttcacgtcc ttcgcccgga tgttgcgatt tttcgccgag
    10681 atgaccccca cggtgatggt gtgctccagg ccaaaggggt tgccgatggc cgccacccac
    10741 tcccccactt ccagtttgtc tgaatctcca agggaaagaa cgggcagatc cttggcgtcg
    10801 atcttcacca ccgccaggtc gaaggtggga tctcgcccca cgagcttcgc cggatactct
    10861 ttgcccgagg ccaacgtcac cgtgatcttg tccgcttcgg ccaccacgtg gttattggtg
    10921 aggatgtacc catccgctgt taccaaaaaa cccgaccccg ccccccgcat gggcacgatg
    10981 tcgctaaagc gccttccctg gtcaccgaag aactccctaa agaaagggtc gtccccgaag
    11041 gggaagggcg tccgggtcac catcttctcc gtgtcgatat tcaccaccgc cggaaaggcc
    11101 tcccgggcga tttttaccac cgggtttccc gtgtagacgt cctgggcccg ggcgtcggat
    11161 agggctccta aaaggatcgc tcccaagacc atcacgccga caaaaacctt ccatttccca
    11221 ctcctgtcca tgatgacacc tcctcgaagg atttcgcccc cagtgtagaa agcttagccc
    11281 tttccgaaaa gggcgttttg cggatctctc cctccgcaag ctcacggacg aaaagatccg
    11341 gccccattgc ccccctaggg gcgactggag ccttctttcc ggcattttcg aaaatcgctc
    11401 caggtcgccc cgagccagac cagccccgcc agggcagcgg tccccgtcag gaaaaagccc
    11461 atgaggtgga tcgtcggcac gttctcgtgg cgccagggat ccacgctcca gcggaggagg
    11521 gcgtagccca ccatgaagag gggccaaagc accgcccctc gcacccggcg ctcgctcccc
    11581 ttcccgatcc accgctccac tcccatcaga aggagcagca gccccagctc tcccagggcg
    11641 tagtagatct gcgtggggtg tcgcaccacc ccttcggggt caaaggggaa atgcatcccc
    11701 caaggaggag tggtttcgac gccgtaacag cagccgttca gaaaacaccc ccatcgcccc
    11761 agagtctcgg tgacggcggc gggcaggctg gcggcttcgg ccacggtttc caggggcact
    11821 ttcaggcgat gacatcgcca tgcggcgcct attcccgccc ccgcgaaggc ggtgatggcc
    11881 gataagcccc cctcccaggg agagacgatg cgccaagggt gggaagagaa gtaatcccaa
    11941 tggtcgtaga ccgcccccag gcgggccccc acgtagaggc ccaccagccc ccagaggagg
    12001 atggtcaccg cccgatcttc gtccagggca aaggggccca tgaggcgccg cttggtccaa
    12061 aacaccgcca gcatcaacga aaaggtccac agtatgtaat aactgtggac cggcacgcct
    12121 ccccacgaga aaagaatggg atacatggac gctatcgcgg caaccgggtg cggtaaacca
    12181 tcttccccgt actggcccgg gccacgttct tctggggcca caccaccact cggcgcgtgg
    12241 gttcttcccc cacggagcgc gtctccacct ctgcgttatc cttgagggcc acgtgcacca
    12301 cccggcgctc ccagctggtc atgggatcca gggatatggg gcgccgccgc cgcaccgcct
    12361 cggaggccgc ttggcgagct gcctcttcga ggtgggcctg acggtgttcg cggaatccct
    12421 cgctgtcgaa gcggacccat cgcccctcca gtggttctcg gagcatgatg ttcaccagat
    12481 attccaaggc cctcaggggt tcgccatagg tccctaggag catctcccgg tctgtgcccc
    12541 gaaggtccac cccgtcttct tcgccgaaga ccacgtccac gtcgaccccc atgcgccggg
    12601 ccatctgtcg caccacttcg cgcacccgca aaagatgccg gggcgccact ggcgccgctt
    12661 ggtaggtatt ttttttcccc aataggccga agaagcgccg cccttcttcc cgcaccgtca
    12721 ccataaaatc ctccggcgcc agtcccagtc gctccgaagc gatctggcgg gcctcttcca
    12781 tcgatgccgc ttcaaagacc accatatctt gttccgccat gccaagcccc tccttaccga
    12841 cgtcccctga actcttccac cgggcggtgg gccccagccc ttcgccctac cggggcttat
    12901 ccttatgcag gacaggtttt tccagcattt ccctttcggt gcggtgatag acccaccact
    12961 gctgccccac gccaatggca gaagagacca cccagtacag caacacgccg ccgggcatcc
    13021 ccaggcatat gaaagtgagg aacagggtca tgaaccagtt catgttcccc atctgggggt
    13081 tagagctggc ggacagctgg gtctgaaacc aggtgagaaa ggcgatgacc accaccagca
    13141 gggcgttggc accatagagc cccacctggg ccaggcccgc gatgttggtg cccatggccg
    13201 acaggatggc catgatcccc acgtcagggc tcgaaccgat gcccaaagcc tggcccaaca
    13261 ccgcataaag cgagctgtgg aggtggatcc ccagaaagga gaaggactcc ttggtaagat
    13321 gtagcaccgt ggtgagcttc tcctgattat tcaacacgtt aaataggagg atgagaatgg
    13381 ggagctggac aaggatcggc aggcatccag cggcggggtt gaccttatgt tccttgtaca
    13441 ggagcatgat ctctttgttc atcgtctcct tgtcgttggc gtacttctcc tggaggacct
    13501 ttgccctagg ttggagtttt tgcatcttct gcatgctgac catttgtttt tgggacaagg
    13561 ggtagaggag gatgcggacc agcaccgtga ggaggatgat ggccactcca tacgacgtgg
    13621 tcagattgta aaagagaacg agaatctgca gcagcagatt ctctcccgcg ctccaaagtg
    13681 cactcacacc gttcaccgcc tgtcgagatt cgcggcagac gccgcgtcct gtgacatatc
    13741 attttctggc atcatggccc ctataccacc gccaacccct cgggcgccgg agacgggaag
    13801 gccttctttg tctccttccg ggaccgggtc gtagcccccg ggatgccagg gggcacaccg
    13861 gaggatccgg cgaaaaccca ggcccacgcc gcgcaccaga ccgaatcgag agatggcctc
    13921 aaaggtgtac tgggaacacg tggggtaaaa tcgacagttc cttcccagga agggggaaat
    13981 gaggcgctga tacccccgaa tgaggaccaa ggccccccgg cttacccagg aaaggcgagg
    14041 acgaccctcc gccgaggggc tcaagggctc cctccgacgg ggacactctt gggcaccaca
    14101 tcgtcccagc gataccgggg aacttcctcc cgcaacagcc ctccccgccc gagcagggat
    14161 cgcaggtcgt cgaagatctc cggcacgggg gctcgcaggg cgtcgttttt caaagagacc
    14221 acgatccaca gcccgtcccg caccaggggg cgcacgtgac gcacggcctc ccggaggaga
    14281 cgcctgccgt ggtttcgcac caccgctccc ccttggcgct tgcccaccac caatccgaag
    14341 cgggcccctc cctcgccccg gtccagaaac agcaaccgca ccagccgtcc ttgagcacgc
    14401 cgaccggtgc ggaagagaag gtcaaattcc catccagaac ggagacgggc cgagggagga
    14461 taggaaaaac gcacccccca tcacaccgcc agacgggcgc ggcccttgcg gcgccggttc
    14521 ctcaacaccc gccgcccggc gggagagctg gaacgcacca ggaaacccat ggaccgcttc
    14581 cggggttttt tatgtggctg aaacgtacgc ttcataaaga cacctcctcc taccacacga
    14641 tgccgtgcag agtcctaacc aaccgggata ctatatcaca gcccgaagac cggggcaagc
    14701 gtcccggctc cttccgccag gtcaagctgg gtccgggcct aggcctccga aagcgatccc
    14761 ccccgccctg tccattttgg ccggggcttt ccgtcccatt ctgcggcggc gggcctctag
    14821 attttttccc gtcgtgggag agtccttggg tgccacgccc ccggggagcg cctgggcccg
    14881 ccttccggag gccttaagca gaggggccga cggagggcgc gtcgcccgga gggcagtttc
    14941 ggaggtcagg gggatcccat ctcttcgttt tccgggaccc ggaggttcat ccggcgcacg
    15001 ggtacccggg gcaggctctc caagatttcc cactccggat agtccgtcac ccggcaatca
    15061 aggataacgg ccctccctcg gtcggaggag cttcggatca ggcgtcccac ggcctgttta
    15121 agcaagagtt tagcccaagg tagggcggag gaagaaaagg cctttctacc cagaagttcc
    15181 tgtcgggcct ccaccagagg gtcttttgga tggggaaagg gaatgcggtc aatgattacc
    15241 tgggtgaggc cgtttccggg gatatctacc ccttcccgaa aggacacgct ccccaccagc
    15301 accgaggttt cgtcctggcg aaagaactcc aggagctctc cccggggaag gtcgccctgg
    15361 acaagcacct tgaaggggcg aggggtggat ttcagcctct cacccaccac cctcaagagg
    15421 cgcatggagc tcagaagcac cagggatcgg ccgccgttat ccttcaccag cctctccacc
    15481 acccgggcca ccagggcgtc ataccccgga tcttgcaccc ggagccccat gcggacgacc
    15541 caaagctcca tctgttgcgc caggggaaag ggggaatcca gcaccagcac ctcgttcggc
    15601 gtcaatcccg tctccgtcga ccagttttcc acggaacctc ccagggccaa cgtggcggag
    15661 gccgccacca ctcctcggca gggggccttc tccatgcttt ctcccaccag atcggcgcac
    15721 tccaccgggg tgctgcatag ctgacggccg tcccaccagt aggcccagga gggaaaatgg
    15781 tccaccgccg tgcaccaggc caggtcttcc cgcatcttcc tcagggcctt actccaggag
    15841 agtatctcgt gcccctcctc cgggatcaat tccggatcgc gctctgccag tggggcgaaa
    15901 cgcttcacca gctgatccgc cagggcgata aggggccccc catccacccc cactccttcc
    15961 tccgcacgac gccaagtgtg gccttcgccc caccggatcc tcatctgtcc gaaaaaagcc
    16021 tccacctccc ggcggcaccc caccagatcc tccgaggctc ccgaaaggtc cacccccagc
    16081 ttttccagcg catcccccgg agaagacacc acccgggctc ccaggaggcg ctgccactcc
    16141 tcctgcgagg tcccccgcga agagctggaa cgagccgcct ccgccatgcg gtgcgcctcg
    16201 tcgcagagga gcacgtcgaa ggggacggga aagggcgtcc ccaggccaag ggtatacgag
    16261 aagaacaggt gatagttcgc caccaccagg gtgctgccct gggcccggcg aagggccgcc
    16321 gtcacggcgc actcctctcg gtgggggcaa gcccttccgg cgcagctcga cgccgaagcg
    16381 gcggcggcca ggagggccgg gtgccccggg ggaaggctga gctcgtccag atcccccgtg
    16441 gcggtgcgct ccatccaggc cccgagcttc cgggtccctt cgccccggtc gccaaaggaa
    16501 agggatccct cggtggtcat ctccaaagcc cggcggcggc agaggtagtg ggatcgccct
    16561 ttgagaaggg tgtaggacac gggaaggccc agcacctgaa ggaggcgggg aatgtccttt
    16621 cgaagcagct gctcctgtaa gggaatgctc gcggtgagga ggagcacacg cccctcctct
    16681 tgggtggccc aaagcaaggc cggggccagg agggcaaagc tcttgcccac ccctgtgggg
    16741 gcctcgacgg ccagaagcca ccccgggggg gcttcctcgc aaagatgccg tacccgctcc
    16801 gccatctgct gctggggggg gcggacttcg tagccatcga aggcccgggc aaagggccct
    16861 tccgggccaa acagggcgta catttccatc atccttcccc tccccacgaa ggggcggcgt
    16921 cacagcttta aaaaaatctt ccggggaagg ccatcccccg atcgccccgc cccccgaagg
    16981 ggaaaagacg gggtatactt tctcccagga tgccgccgaa ctcagggcac cccgggaaag
    17041 gagtccgtca tgaggcgtct tgccgtaccg ctgcttttga tcgccctcgg gggatcgatc
    17101 gtcgggggcc atctccccga ggacggaagg gggtcttccc cggcggaggc cttttttctc
    17161 ttcgaggagg aagacccctc ggcgcccccc gagcccttca cctggcgtcg ggccctcccc
    17221 ttggacgacg tggctcgtct ctttcgggag gggggatttt tggcgctctg cgagggtgga
    17281 agttcctccg acgtggcggc ggcccttctt cggggggccg atcccaacct gtccaacgac
    17341 cggggacggt cggccctcat gttcgccctg gcaaatcccg accccgccgt ggcccaaatc
    17401 ctcctggaag ggggagccga cccccgggcc gtcgaccggg agggacatcc cgtgtggatc
    17461 ttcctccccc ccgggggggc gctacgcccc ctggccctgc tcctggactt cggactaaac
    17521 ccccggagcc ccggggcctc gggggtcgaa atgcccttta tcctcgccgc cgccgaggac
    17581 gcccccgcca ccctgcccct gctcctggat cgaggagttc ccgtcgatct ccgggactct
    17641 gccggggcca ccctcctcat gtgggccgcc accaaccccc ggccgggggg cgtggaggcc
    17701 ctgctcctcc ggggggcgga ctcccgggcc gtcgatggcg aggggcgaac cccccttcat
    17761 tacgccctgg agagccccta tcccggaggg gaagtcgtac gggccctgct ccggggaggg
    17821 gccgatccca atgcccgtac cgtcgaaggc accactcccc tggcgttggc ggcccgacaa
    17881 ggggcccccc gggaggtctt cgaagccctc cttcgggggg gagccctgcc cttcctgggg
    17941 gatgaagagg gcctcacccc ctgcgacgag gcccgcacga accccgacct gaggcatcgc
    18001 cgggatatcc tgaagcttct ctgcccctaa aaaggggatc ctggaccgga tcccctcagg
    18061 ggcgcactgt ggagataaag tctcctcgca cccacccttc gtggccgctg ggagtccgca
    18121 cgtaatgcca cagggcgtcc gggccggtcc ccaccgcatc tcgctccacg taggcggaga
    18181 ggtcccgccg aaagaccacg gggaccatcc cttcctgctc ttttcccacc acccgtaccg
    18241 aggtgccctt gcgcaggtcg tatttttttc ccaggcagaa cacggacacg gcgcgcagga
    18301 gccgggcgtc gtagtactcg ggggaagact ggtacaccgt gagggattcg tttcccgcca
    18361 gattggtcac cacccgggat ttcgtggtgg gctcctggcg cacgtttacc ttggtccccg
    18421 tgagggcccc cggcaccccc cgggcctccc ggcgggcctc caaaaaacga gggccaaagg
    18481 atcccagctg ggtctctcca aaggccaggg cccgaagcga ggcgtcgtct ccccccccgt
    18541 cgtagaggca gagcaccccc cgatcatccc ccgtcccctg gggaaaaaaa tcgaaggggc
    18601 ccagggattc cggggtctcc cccaggagat ggcgcagggc ggagcgcccc atccccacaa
    18661 aggtcccggc gaagctgcca tcgccggaga actcgtactg caggagctcc cttcccgcat
    18721 ccttcacgcg aaagacgtag gaaaagccct ctccctgggc gcggcgcacc tcccggggtt
    18781 cgaaagatat cctcaaaggc acctcggtgg tccccgggac cggatggtcc atcaaggggt
    18841 cttcggcgct ccgagccccc gcctcacgga agaggcgatc cagatccacc gccgccaccg
    18901 caccggggga aagggaggcc ttcggatttt ctcgccgcac cgagggtccg ggggtgggag
    18961 aggtgccccg gagtccgggg tcccggggat gccaaaagaa aaaatacgtc cccacccccg
    19021 cccccaagaa ggtcagcacc atccctatcc cgaggatctt caacacgtgg tatcggtagt
    19081 gcgcgctgct tcgtttcatg aggccctccc gatctgcccg ttccgcctgc ggcaaacggc
    19141 cgttctcgcc ggcgatcgta ccaccccctg ggggggcacg gtccctactc tcgtcattgt
    19201 acgccctgat ctcgtggggt ggaagcctct gacgggaaaa gacgtaaaaa agcggagccc
    19261 cgtcggggct ccgcctcgct cctttaggtt ggtgccggaa gggctcctta cgccatcacc
    19321 acttcggtgc cttcctgtcc ctccagggcc tctaccgcct tgtaaaggga ggtaatgatg
    19381 gccttcttcc ccgggaaggt gcgggcgaac ttcaccgccg ccttcacctt ggggagcatg
    19441 ctccccggag cgaaatgccc ctcgtccacg tactgaatgg cctcgggcac cgtcatgcgc
    19501 cgcagatcgc gctggtcggg ctttttgaag ttgatggcca cccggtccac ctcggtgagg
    19561 atgaggagca catcggcctg gatgtcctcc gccaggcgct ccgccgccag atccttgtcg
    19621 atgaccgccg ccaccccccg gagggagccg tcccgctctt ccaccacggg aatgccgccc
    19681 ccgccgcagg tcaccacgat ggtggtgtcc cacagacgct tcaccgcgtc gatttccaca
    19741 atgcgcacgg gcgcggggga gggcaccacc cggcgccacc cccgccccgc gtcctctttc
    19801 atgttgtagc ccttttcctc ggcgatgcgc tgggcctctt cttcggaata gaacgctccg
    19861 atgggcttcg tggggttccg gaaggcctcg tccttggcgt ccaccaccac ctgggtggcc
    19921 aacgtcacca cgggaacgtt ccgattgcgc cgccggaggg cgtcccgcag ggcctgctgg
    19981 atctgatagc cgatgtaccc ctggctcatg gcgccgcaca cgtcaaaggg catggcgggg
    20041 gtttcgggat tgaccttcgc cgccgtctcc gacgccagga cgatgcgccc cacctggggg
    20101 ccgttgccgt gcaccacggc catttcgtag ccccggcagc tcaactccgc cagatactcc
    20161 accgtgcggc gcaccacgtc gagctgggct tccgccgtgg cgggcgttcc cgcctcctgc
    20221 aacgcattgc ccccaagagc caccacaact tttgtcgtac tcacgagaaa ttcctccccg
    20281 agaagatctt cagatgaacg gctccgccgc caacgaaaaa attatagcgg ctcccggcca
    20341 aaagtccata gaaatttggt tatatataaa ccaaattcga gaaaaagttt attcgcccct
    20401 ctccggaaga gagagggaac ccgccccccc ggcgacaggc ccttgctccg gagaaaatcc
    20461 gaaggcgcaa gcccccggcc cggtcttctt ccggcctcgc cccatgttcc ggcttttcgc
    20521 cgcgcctcct cccggcgttc tcctccggag gcgccggaga gaacggggag cggcggaggc
    20581 ttccgcaagg gggtctgcgg cacgccgatt ctttcggtcc ccgagggctc tacgggagct
    20641 ccacccccgc caggagcgtt ttgatccatc ccttggcccc gtcgtaccgc tccgccggca
    20701 gcaccacctc caccagaagg ggtgtgccgg ggtaggtggc gatcatcgtg gctttccgct
    20761 gccccgaggc ctccggtgcg gcgtagagcc ccgcccgaca gattttgccg acggcgtttt
    20821 tcagggtgtt gaccccgaag aacgtctgcc ccgctccccg ccgcgtcgcc tccgccgaag
    20881 cctcctgctc gccggccagc accagcacgg agatcacgga catgtccggg ccgctgtaca
    20941 ctcctttgga ggaggtcact tcctggggca ccgaggcatc ggggagccgc acccggatct
    21001 ttttcgaggc gtccacgaag agccccggat cggggggttc cgtcaggtcg gggagctctc
    21061 cttcggagag atccggcgag agggcgggaa ggtcatcgcc gctggaaatc gcccccactc
    21121 ccgtcacggg aggcgggggg gtgaggggca ccaccgaggg cccgggggat cccgtggccc
    21181 cctgtcccgg aaagagaggg ggcttgcttg tgcccgtgcc ttccgggaag accgcctgca
    21241 gaaagttcgc aaactgggcc tccagcgcgg ggaagtcctt gcgcaggccg tcgaagagaa
    21301 tggcccgggc ggttttgccg tccatgaaga ggaagagccg ggccttgagc tccgccgccc
    21361 cctcgccctg gccggtgaag gtgaagtcgt gacgcaccgc gggccgtcct cccacggtgg
    21421 cgtcccccac ctcccccgcc gcgtagttcc tgaaggatcc cgtcatgagg ctgttggcct
    21481 gaaaggcctg gaagtagctc cgcacgtcca tgggggcggc gagctcttcc gtgaagaaaa
    21541 atgcctcggc ccagaccgat tttcccacct ggagcacgta gtacgacgcc acccccgggg
    21601 cgggggcggc gcgctcctgc caccccggcg gaaggggccc cagacgcagg gccgccgtcg
    21661 ccgccccgga gcccaaaagc agcgccacgg cggcaaaggc cccccacaag aacctctttc
    21721 tcatctccac cacatccttt ctcgccctcc ggcgcggaac ccggagccac gacagagctc
    21781 cggagcgcgc ctccgggatc agtatggcac gaaatcgtct tcggcgccac gggaaggggc
    21841 gaagcccttt ttgcccccac gaaggggccc ggtgacctcc gcgccggaga acaaaaaagg
    21901 gggggcacaa ggccccccgt gatcttttta tattacgccc agaacaaacc tacttcgccc
    21961 gcatgtaggc ccagacgtgc gcggccgtaa agtctaccga tcctacggtt cccttcgttg
    22021 ccgatccgac aatgacaagc ccttcctgcg ctctgatatc ggcaagcctc gcccgggacc
    22081 ccgccgtggg cacatagaca ccgacccacg cctggtctcc ggagcccgcc acgtcgtagc
    22141 ttccacccaa cgtcttattc atcatcctgc tcaggtcggc caccgtaggc agggccgcct
    22201 gggccccatt atcagcgtag tactgcatgg tggagctcgc catggtgcgg aggtcgctca
    22261 gggccttggt ggcgtcggcc ttgtccgtgc tgctccccat gaccatcatc atgccgccgg
    22321 cgaggatgcc gatgatgatc atgacgatga gcagttccac cagggtaaag ccgcgctgct
    22381 tcttcatgag tttcttcatc acaataagtc ctccttttct catcaacaaa acatcacata
    22441 aagggataaa aaaggtgccc accacgcctc gtcttcttca cctcgcccga atccgcgcgt
    22501 ctcaccccct ttcatgcccc cggaccgggg gctagagcat ggtctgaatg gccccgatga
    22561 tgggaaagaa gatggccatg gcgatgaccg ccaccacgcc tcccacgaaa acgatcaaca
    22621 tgggctccag caacgagttg agctgtccca ccttcacgtc caactccgcc tcgaaccagt
    22681 ccgccacctt gttcatcatc tcctccaggc gcccggtctg ctcgccgatg gcgatcatat
    22741 gggccaccat gaggggaaag atcttgaccc ccttggcggt ctctcccagc ccttgccccc
    22801 tcttggcggc ctctcgaagc atcgcgaagc cctggcgcac ctcgtagttg tccgacacct
    22861 ctcccgccat ctccagacaa ctcaggatgg ggacccccgc ctggaccaga gatcccaggg
    22921 tccgggtgga gcgggccata atggccttgt actgcaggtc gccgaagacg ggaacccgaa
    22981 gccgcaccct atcgacgaag gggcgagtgg ccttactgga ccccaggacc acccagatcg
    23041 ccaccagagc cacgatcgct cccagaataa tataccactt gttgccaaaa aagtccgcca
    23101 gatcgaagat caccgtggta atccacggca gctcggtgtt gaggttgtcc agggcgcctt
    23161 taaacttggg gacgatgacc gtcaccatga ggtagagcac cagggtggca aaggtcatga
    23221 tcaccgtggg gtaggtgagg gcccccttga tcttgcgctg gagggtctct tgtttctcca
    23281 gaaaggatgc cagacggttc acggtgacgt ccagtagccc cccctcctcc cccgcctgca
    23341 ccatggccac catgagggtg ttgaactcgg ggcgattttt catggcggcc gccagagaca
    23401 cgccgttatc gagcattttt ttgacctgga ccacggcgta gg
//
LOCUS       DAOOER010000030        22948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_2604_length_22948_cov_8.635347, whole genome shotgun sequence.
ACCESSION   DAOOER010000030 DAOOER010000000
VERSION     DAOOER010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 22948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22948
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2604_length_22948_cov_8.635347"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /gene="hisIE"
                     /locus_tag="PL162_05515"
     CDS             <1..630
                     /gene="hisIE"
                     /locus_tag="PL162_05515"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HQF91514.1"
                     /translation="PQGLVPVVVQDAQSGAVLMVAWANAEALKLTAETGEMVFFSRSR
                     GRLWHKGEESGHRMAVRELRRDCDGDTLLALVEPRGPACHTGETSCFHLPLSQERTAD
                     PAFLGRLWRILRERRHASPETSYTARLLASGLSRVAQKVGEEGVETALAVALGKKDEA
                     VSEGADLLYHLLVALVALDVPLDALWQELASRHCPAPGTEAPEKTPGER"
     gene            complement(736..1512)
                     /locus_tag="PL162_05520"
     CDS             complement(736..1512)
                     /locus_tag="PL162_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HQF91515.1"
                     /translation="MEETLRPYRVELHCHSVLSPCAELEMGPRDIVARAREQGIELLA
                     LTDHNAMRNVPALVTCAAEAGGPAVLFGLEVQTAEDIHVVALFPHEEAARGFEAWLWT
                     GLPPVPHRVEAFGYQLVVGPDHEVIEEVETLLVQGVSFSVDEVIRRIREDGGLSILAH
                     LDREAFSYWAVLGPVPDDLPVDAVELSWRLSRESAERWRRHFPGRTVVRSSDAHRLED
                     LAVRRGCRMMLAEPTFDEVARALRGEGGRRVYWPWGETEG"
     gene            complement(1621..1962)
                     /locus_tag="PL162_05525"
     CDS             complement(1621..1962)
                     /locus_tag="PL162_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine kinase"
                     /protein_id="HQF91516.1"
                     /translation="MTVREVLEKIGAEVLCDAGADRPVKGAVVGDLLSFVMGNASEGA
                     VWVTVVAHANVAAVAVLKDMPCILIAADREAAPDFIERCRREEIAVGRSPRSPFDLCG
                     ALWELGLRGEG"
     gene            complement(1959..3257)
                     /locus_tag="PL162_05530"
     CDS             complement(1959..3257)
                     /locus_tag="PL162_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HQF91517.1"
                     /translation="MMHGIKIFNAACKGCVHCIKSCPTEAIRVIDGLVHILPDLCIDC
                     GECLRTCRKKALGLDEDDWRELLSQQVPVLADPSFFAQFAAWSPDGAPEALEMAGIKT
                     LDSRRAFDLAALAVARCLDEAPREELPLISTYCPAVLRLIRIRFPELLSRTVPAESPL
                     EVAAELWARESGGCPPVLLAPCPAKITMVREPLGRDRSAIGAAVSVRRVVRDLLSSGI
                     KNHSPRAELSSRRDLLWALRGGEAEHVRRFARRPVRAIAVSGLRNTLDVLRDLELGRL
                     QGVDYVECRFCDLGCIGGVANAESRFLAEIRVKTTEVSWDLSEEDREELEHLHASGIW
                     RLRKDIKPQNRLPLGGDFSEAMGQLARFREVLGQLPLIDCGACGRPSCQAMAEDIVRG
                     NAEVTDCVFKLRERIAALAGEIEGLSRNVPHTLRTREKES"
     gene            complement(3275..3721)
                     /locus_tag="PL162_05535"
     CDS             complement(3275..3721)
                     /locus_tag="PL162_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HQF91518.1"
                     /translation="MKDPVVLDFPVEGEDFLAAGEASSSVKDTLKMLGVPSSICRRAA
                     IITYEAEMNLVIHAGGGMLRARIDEEKVVLVTEDQGPGIADIDQAMREGYSTASDQVR
                     EMGFGAGMGLPNIKRNADRLDIESTVGQGTILTASIYFVGEPQPSA"
     gene            complement(3718..4098)
                     /locus_tag="PL162_05540"
     CDS             complement(3718..4098)
                     /locus_tag="PL162_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HQF91519.1"
                     /translation="MKVQEIGAILEGEFLCGGEWAENMDVDFGYAADLMSDVLAFARA
                     GSMLITGLTNIQIVRTAQMMDIPAVVFVRGKRPQEDTVRLAAQSRIPLLVTMKSMYET
                     SGVLFAAGLPACIIPHREAGGEEH"
     gene            complement(4137..5324)
                     /locus_tag="PL162_05545"
     CDS             complement(4137..5324)
                     /locus_tag="PL162_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91520.1"
                     /translation="MFFMILGLGLGLGEPLPEARGQKKPSPDAGAEGWLWRVVVFPPF
                     EGWDSPAGKSIWAALQMAAEESNERRYGLQNHDVEFLREEMPPLEDLASHLRERLPRW
                     KKDMVVGFFSFADDEPDRVLVQELAGTEFPLLLGFGEGVSLRSGDAPFDHAFALDLYR
                     RFRVRALAEYATRRLDPAAGITVLGDRLDPYLAGTAELFIQDLLFRELSVLPLWAMGA
                     GDRDLGSRLREAVAVGSSVLVSALDTMGTLDAWRFIREEHLPLALWYLGSLNGHLVRR
                     PGILAADQRYALEHDPTLRDFRGRIFDRTRFRVADPPMAARAYALAWWFFQGVDEARG
                     VGGALLAALGRSKKIPLGEECPDIDPGTHRPLGRMVTVFQSDGESWRPMMEMRIRSRT
                     IPE"
     gene            5627..7018
                     /locus_tag="PL162_05550"
     CDS             5627..7018
                     /locus_tag="PL162_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HQF91521.1"
                     /translation="MEVFGLSATAVIFSFALLGVVRGRKTSGGDFALSGRRVSSRGVS
                     GILLGALVGGASTVGTAEMAYVHGLSAWWFTLGGGLGCLLAGLFFARPLRRAHIVTIP
                     QFLGQAYGPATALVALAGSSLGTFLSVLAQFLSGEALLRSVLPLGAGGSAAVLGILVA
                     AFLFAGGLRSYSAVGSAKIVFLYFTLILCGGAAFLQGGTPGALIRDLPPFPYLSLFSR
                     GVSRDLGAAGSLVVGVLSTQIYLQALFAAKDETVARRGALWSALLMPPLGILALWVGL
                     HLRWAGVEIPPSQALPHFLRTAFPPALGGALWGGIFITVLGSAAGLVFGIATNLVQDL
                     MIPWASRDFSGKSLLWTHRGAVGAVLMAAALVELSLEKSMILDWSFLSMGLRGAGAFF
                     PLVWAVLRPGVLPGPWALASGLGGLGAALLAPFFPFPVEPLFAGLTVSGLLALGGVIA
                     GRGRPTPLRTPPA"
     gene            complement(7266..7763)
                     /locus_tag="PL162_05555"
     CDS             complement(7266..7763)
                     /locus_tag="PL162_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HQF91522.1"
                     /translation="MAEIKGWFVEQLSIPEGCNIILGQSHFIKTVEDLYEALVTSSPS
                     LEFGIAFCEASGPCLIRRDGNAPDLVQAAVQNAMQLRSGHTFIILLRHGYPINVLDRI
                     KAVQEVCRIDAATANPLQVIGVETDQGRGVVGVVDGFASRGVEGNEDEESRKKLLREI
                     IGYKR"
     gene            complement(7797..8924)
                     /locus_tag="PL162_05560"
     CDS             complement(7797..8924)
                     /locus_tag="PL162_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQF91523.1"
                     /translation="MTTPQQDHLGSSGDPRKRFDVMEALRRNVVPLLFTVLCVVGAYY
                     SGLTLPFLINEIIVRMARNSFLVISLIIPILAGMGLNFGIVLGAMAGQFAFIATANWE
                     IGGFVGLILTVILSIPLAILFGWLTGKLFNNSKGKEMITGIILGFFANGVYQFICLYI
                     LGNFIPIRVASMLIPGGIGLRNTIDLKQIHYALDNIWKISISFGSGVPVTIPVVTLLV
                     IVALCVGMVLLFKTKLGQDFRAVGQNRHIAEVAGIPVDRVRVLAIIFSTVLAALGQII
                     FLQNLGNMNTYGSHVQVGTFAVAAILIGGASVSKATIGQALMGILLFHTLFIVSPLAG
                     KNLLGNVQVGEFFRVFVAYGVIGVALALHAWQGVRRKKGGA"
     gene            complement(8921..10012)
                     /locus_tag="PL162_05565"
     CDS             complement(8921..10012)
                     /locus_tag="PL162_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQF91524.1"
                     /translation="MLLNALPSPEGGESFGDRLGAAVRDFGIPRIIILGFLGVLLATA
                     VSLRLPMGQLFGNILTRFGMNGILVLAMIPSIQSGTGPNFALPLGVTCGIVASTLSIE
                     LDLRGFTAFFFAVGVAIPLGAISGYLYGVFQNKVKGQELTVGTYFGFSVVSLMCIFWT
                     VVPYKSPEMIWPYGGSGLRVTITLTNRFAQVLDGFMSFKIGTMTVPTGLLLFFGMFCA
                     LLALFLRSKTGVAMMMVGSNPRYAESAGLNVDKYRIIGSVVSQALGAVGIIVYAQSFG
                     FIQLYLAPLFMAFFAVASVLIGGASLRRATVWHVLIGTFLFQAILVVSMPVANKVVAD
                     NLAEVIRIIVSNGIILYALTRKEAGVDVR"
     gene            complement(10003..11664)
                     /locus_tag="PL162_05570"
     CDS             complement(10003..11664)
                     /locus_tag="PL162_05570"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HQF91525.1"
                     /translation="MRGIAKEYFGNRVLKGVNLRVGPGEILSIVGENGAGKSTLMNIL
                     FGMPVIHNTGGFEGEILFDGAPISIRSPQQAVELGIGMVHQEFMLLPGFSITENIKLN
                     RELTRPNLLSRLCGPKLEHLDMPAMRSDSRASLDRMGMSIDEMLPVAGLPVGHMQFVE
                     IARELDKKKTRLLVLDEPTAVLTESEAQVLLNAMRSLAAQGISLIFITHRLDEVVQVS
                     DGITVLRDGEQVADLSPRETSVGHLAELMVGRKLEAVGCDGCAPSPRTDQVLLRLEHL
                     EVDMPGEMVKDLSLEVFQGEILGLGGLAGHGKVGVANGVMGMYPARGIMAIEGTPLPL
                     GHTLEALRSGLAFVSEDRRGVGLLLEESIEMNVAAPAMQSQEKFLRKVGPRGFQFSIM
                     DRRAMRRHAERFIEELDIRCTGPEQIVRRLSGGNQQKVCIAKALTLEPRILFVSEPTR
                     GIDVGAKKIVLDLLVRLNREHGVTVVVTSSELAELRSICDRIAIVCEGRLEGILPPDA
                     SDRDFGLLMAGEKSHRGRDAEGPSHLSGSCGQGSGQGSKEESPCS"
     gene            complement(11799..12911)
                     /locus_tag="PL162_05575"
     CDS             complement(11799..12911)
                     /locus_tag="PL162_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HQF91526.1"
                     /translation="MKKSMLSFLVMALVVCFCGAAFAGLPKVGIMTGTTSQGEEEYRA
                     AQKMINDYGADRIVHVTYPDKFMDEQETTIQQILSMAQDPDVKAIVLVQGVPGTAAAI
                     DKVKEIRPDMFFINIVPHEDPMLSAQKADIVLETDNPTRGVTIVELAKKMGAKTFIHY
                     SFPRHMSYPLLANRRDIMVEECKKQGLEFVFVNAPDPTGDAGIAGAQQFILEDVPRQV
                     EQYGKETAFFNTNCGMQEPLIKAVMTTGALYPEQCCPSPYHAYPGALGIEIPADKAGD
                     VDFIIEAIDAKVKEMGGAGRFATWKSPANMAMTYIATEYAFGIGDGSIKGFDRAKLEE
                     LFKKYAGDVRLSTYQSGADKADNFILFIGESKIFGL"
     gene            complement(13266..13661)
                     /locus_tag="PL162_05580"
     CDS             complement(13266..13661)
                     /locus_tag="PL162_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91527.1"
                     /translation="MKARRLVVRAAALFLFVALGVFLFVNGKGHDILLDNKTVTMGDQ
                     TYEAFASALISLDGKKAVEIQRRERLPEMLVGKNHVIRVEVMNRGGKVIQTREEKFSL
                     GLDRTWLISLPMLISGDARWIQVFKTGEN"
     gene            complement(13680..14792)
                     /locus_tag="PL162_05585"
     CDS             complement(13680..14792)
                     /locus_tag="PL162_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQF91528.1"
                     /translation="MKNKSFKDILISNAVPIIFLVLSAAAIPLSGFTTSYLIQEMVTR
                     MGRNCFLVLSLLIPIAAGMGLNFGMVLGAMGGQIAIILVTDWNIVGMQGLLLAAMIST
                     PISLALGWLTGEILNRAKGREMVTSFMLGFFMNGVYQFIVLYGFGMVIPVENHAMLLS
                     RGFGIRNAINLQGVRQVLDKFLMVDIGPIHVPMAAFIVIALFCLFILWFNRTKLGQDM
                     RAVGQDMAVAKSAGIAVDRTRIIAIIISTVLAGYGQIIFLQNIGTMNTYNAHEQAGIF
                     AIASLLIGGATVSRATITNVFVGVMLFHLMFVVSPMAGKTLMGQAQLGEYFRVFVSYG
                     IIALALVLHALRRRMDKERERQSLRGGDAPAPANGH"
     gene            complement(14789..15829)
                     /locus_tag="PL162_05590"
     CDS             complement(14789..15829)
                     /locus_tag="PL162_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQF91529.1"
                     /translation="MKKIRAFIEAAGLPRVIIGGFLVLLFAVAPLAQISVARSVSDTL
                     VRMAMNGVMVLALVPMVQSGCGLNFGLPLGIIAGLLGALLSIELDITGFAGLFFAMGV
                     SLPFAAIFGYGYGLILNKVKGDEMMIATYVGFSSVSLMCIAWQLLPFTSPIMIWGYGG
                     SGLRVTVSVSEHWLHVLSDFLSFQIGNFYFPTGMFLFLAFMCFLVWGFFRTKIGTAMT
                     AVGSNPEFARASGIDVDKMRTISVVLSTMLGAIGIVVYQQSFGFLQLYTGPFYMAFPA
                     VAALLLGGASVNKATLFNVIVGTFLYQGICTMTPSVINSLLQTDMSEMIRLIVSNGMI
                     IYALTRKVQVKR"
     gene            complement(15837..17444)
                     /locus_tag="PL162_05595"
     CDS             complement(15837..17444)
                     /locus_tag="PL162_05595"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HQF91530.1"
                     /translation="MTQEPLLRMEHIGKEYFGNRVLADVNLTLQRGEILGLVGENGAG
                     KTTLMKILFGMPVIKETGGYEGKMYLDGKEVNFDSPFDALDGGIGMVHQEFSLIPGFS
                     TTENIMLNREFTCYSPLVELFGERLQTLNRPAMRQRAAKAIDTLGVRVDPDMLIQEMP
                     VGHRQFTEIAREIDRESTKLLVLDEPTAVLTESEAAILVKALRKLADSGIAIIFISHR
                     LQEILDLCDKILVLRDGMVIKEAPTKGTEIRQIASWMVGRTVEDKKQDDAVRVCLEPI
                     LRVDHLWVDMPGETVRDVSFDVCRGEIFGIGGLAGQGKLGIANGIMGLFAAGGAVTWK
                     GKALKLNDPHGALEAGMAFVSEDRRGVGLLLDEPIDWNIAFTAMQVQGRCLKSLLGGL
                     IQWRDDVAMASLAQEYIDALGIKCVSSRQRAVELSGGNQQKVCLAKAFAVGPELLFVA
                     EPTRGIDVGAKKLVLDTIRETNRHKGTTVVMISSELEELRSICDRIAIVDEGRLAGML
                     SATASVEDFGLLMLGAGKGPEVAAGVR"
     gene            complement(17680..18900)
                     /locus_tag="PL162_05600"
     CDS             complement(17680..18900)
                     /locus_tag="PL162_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HQF91531.1"
                     /translation="MKRGIVFLALIALVTFFAGGALADAPFHIGIMTGTLSQSEDDLR
                     GAEKLIEMYGDVANGGMIKHVTYPDNFSSEMETVIAQLVGLADDPLMKAIVSVQGVPG
                     TTEAYRKIRERRPDIILLIGMSQEDPNLTTQAADVVTDPDNVARGYLIVKAAQKLGAK
                     TFVHISFPRHMSIELLSRRRAIMEEACKDLGMTYAFETAPDPTSDVGIPGAQQFILEK
                     FPAWLDKYGKETAFFCTNDAQTEPLLKRVAEMGGFFIESDLPSPLLGYPGALGVDLSS
                     VQGDWPAILKRVEDAVVAKGAAGRMGTWAFSFAYTTGVAMGEMGKKLVEEGKTPEDFK
                     NGNIDIKKEVLEAYGKFSPGAEWNASFYTDASTGVTMKNYPLLYQDTYIFGKGYMKMT
                     DEVVPDKYFTVTGQ"
     gene            19438..20553
                     /locus_tag="PL162_05605"
     CDS             19438..20553
                     /locus_tag="PL162_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQF91532.1"
                     /translation="MAFAGMAMAADDVIKVGYLAALTGDWAAYGQTEANSAQLAVDEI
                     NANGGVLGKKIELVVSDFRTRPEDAVNAVRRLVQEDKVVAIIGANGSGINIATAPIVN
                     EAKVPQIGTVSTNPSVTVDKDGKVRPYSFRICFTDPYQGKLIAYFAAKELGKLKGAIL
                     YDVASDYSQGLREFFLKSYAEYGGEMVADLGFQGGTDVDFRAQLTEIRDKGADVLVLP
                     NMGKEMALTMKQARELGMNDIIFVGGDGYGEFMWEIAGDAMENSYWINHVAPEDPAMA
                     PFFADYKKKYNDECKEFTNGILAYDAMYWLADAINRAGEVDSTKIAQALESSVGVQLH
                     HATLTVDPTDHNPKDKDAVVLVAKDGKGQFFKKIKPE"
     gene            20661..21539
                     /locus_tag="PL162_05610"
     CDS             20661..21539
                     /locus_tag="PL162_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQF91533.1"
                     /translation="MGIFLQQIINGLSLGSVYALIAVGYSLVYSVLLFSNFAHGGFLV
                     VGGYACYFALKTLGFNIWISSLLSLVGAGFVAILVERLAYKPIRERTNVTLYLLIASM
                     GMSIVIENIFVVTVGGRFRALPPVIPTAPVNLGIATTSAFDIISLGVAVVCLVALQIF
                     LSRTKWGLAIRAAAYDLRTAGLMGVNVNRLISIVFFVAGALAAVGGIFLSVRYTLYPQ
                     LGTITIKAFVAAVIGGLGSLPGAVVGSLILGLSEMLTAGFISSQLRDLVVFSLLVITL
                     LVRPTGLFGKSVGEKV"
     gene            21552..22430
                     /locus_tag="PL162_05615"
     CDS             21552..22430
                     /locus_tag="PL162_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQF91534.1"
                     /translation="MSPYVESIITLLMINSIAAMGVSLLTGFTGIFTLGHSAYMALGA
                     YTTAILHMGHSVPWAIAIFAGAAFAMLVAWLIGVPTLRLMGDYYAIASIGLAESIRLI
                     IANWQSLTRGARGYPDISKFATFNVTLAFFLVMGALIFLLIHSRYGRAFKACRDDHVA
                     ASLLGYNTARYRVLSLVISAFYCGIAGGLLAGFLQYLKPDMFDMLKSTELTAVVVFGG
                     MGSMSGCILGTTIITLVTELFRPISQYRMLIYGAVLVIIMVLRPEGLLGRYELGPRLL
                     RHLFGRKTPEGTEGRG"
     gene            22434..>22948
                     /locus_tag="PL162_05620"
     CDS             22434..>22948
                     /locus_tag="PL162_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQF91535.1"
                     /translation="MGALLEVKDMNKFFGGVQAVADMSFTLEKGELMGLIGPNGAGKT
                     TIFNLITGVYPLSSGSVFFRGEDITGGKTYDIVKKGIARTFQNLRLFGASTVLENVMT
                     ACQQHYPYSFLEAISHLGRWKSQEKNIRNKSMELLERVDLAREAHRAAGTLPYGHQRR
                     LEIARALALQP"
BASE COUNT         4407 a         7478 c         6788 g         4275 t
ORIGIN      
        1 ccgcagggcc tcgtgcccgt ggtggtccaa gacgcccagt ccggcgccgt gctcatggtg
       61 gcctgggcca acgccgaggc cctgaaactc accgccgaaa cgggggaaat ggtctttttc
      121 agccgatccc ggggaaggct ctggcacaag ggggaggaga gcgggcaccg catggccgtg
      181 cgggagctcc ggcgggactg cgacggggac acgctgctgg ccctggtgga gccccggggc
      241 cccgcctgcc acacggggga gacctcgtgc tttcacctcc ccctctccca ggagcgcacc
      301 gccgaccccg ccttcttagg gcgcctctgg cgcatcctcc gggagcgccg ccacgcctcc
      361 cccgaaacga gctacaccgc ccgcctgttg gcttcggggc tctcccgggt ggcccagaag
      421 gtgggggaag aaggggtgga gacggcgctg gccgtggcgc tgggcaaaaa agacgaggcc
      481 gtctccgagg gggcggacct gctctatcac ctgctggtgg ccctggtggc cctggacgta
      541 cccctggacg ccctgtggca ggagctcgcc tcccgccatt gtcccgcccc ggggaccgag
      601 gccccggaaa aaactccggg agagcggtaa aacggaaagc cccccggccc tttaggaccg
      661 tcggggcttt cgcgaagaag gtagggggag agcgaaaaag gcgaaagacc tcggcccccc
      721 cggggaaggg gggcgttatc cttccgtctc gccccagggc cagtacaccc tccgcccccc
      781 ttccccccgg agggcccggg ccacttcgtc aaacgtgggt tccgccagca tcatgcgaca
      841 cccccggcgg acggcaaggt cctccagccg gtgggcatcc gagctccgca ccaccgtgcg
      901 cccgggaaag tggcgtcgcc agcgctccgc cgattcccgg gataggcgcc aggagagctc
      961 cacggcatcc acggggaggt cgtccggcac cggccccagc accgcccagt aggaaaaggc
     1021 ttcccggtcg aggtgggcca ggatggagag ccccccatcc tcccggatgc ggcggatcac
     1081 ttcgtccacg gaaaaagaca ccccctggac caggagggtt tccacctctt ctatcacctc
     1141 gtgatcgggg cccaccacga gctggtaccc aaaggcctcg acccggtggg gcaccggagg
     1201 gaggcccgtc cagagccagg cctcgaatcc ccgggcggcc tcttcgtggg gaaagagggc
     1261 caccacgtgg atgtcttccg ccgtctggac ctccaggccg aagagcacgg cggggccccc
     1321 ggcctcagcg gcgcaggtca cgagggccgg gacgttgcgc atggcgttgt gatccgtgag
     1381 ggccaagagt tctatgccct gctcccgggc tcgggcgacg atgtcccggg ggcccatctc
     1441 cagctcggca cagggggaga gcacggaatg acagtgcagt tccacccgat agggccgtaa
     1501 cgtttcttcc accggttttc cccccttata ccagatacca ggcggtggcc agatcggacg
     1561 ccccgccccc ggaacaccac cccggggcaa gggaggagtg cggagccccg gcccgtccga
     1621 ttacccctct ccccggagcc ccagttccca gagggccccg cagaggtcga aggggctccg
     1681 ggggcttcgc cccacggcga tctcctcccg gcgacatcgt tcgatgaagt ccggcgccgc
     1741 ctcgcggtcg gcggcgatga ggatgcacgg catgtcctta agtacggcca ccgccgccac
     1801 gttggcgtgg gccaccacgg tgacccacac cgccccctcc gaggcgttgc ccatgacgaa
     1861 gctcaagaga tctcccacca ccgcgccctt caccgggcga tccgccccgg cgtcgcagag
     1921 cacttcggct ccgatttttt ccaacacttc tcgtacggtc atgactcctt ctccctcgtc
     1981 ctcagcgtgt ggggcacgtt ccgggacagc ccctcgattt cccccgccag agcggcgatg
     2041 cgctcccgca atttgaagac gcagtccgtc acctcggcgt ttccccggac gatgtcttcg
     2101 gccatggcct ggcaggaggg gcggccacag gccccgcaat cgatgagggg gagctggccc
     2161 aagacctctc ggaatcgggc gagttgcccc atggcctcgg agaaatcccc ccccaggggg
     2221 aggcgattct gcggcttgat gtccttacgg agccgccaga tccccgaggc gtggagatgc
     2281 tccagctcct cccggtcttc ttcggagagg tcccaggaaa cttccgtggt cttcacccgg
     2341 atttccgcca aaaagcgcga ctcggcgttg gccacgccgc cgatgcatcc caggtcgcaa
     2401 aagcggcatt ccacgtaatc cacgccctgg agacgcccca gctccaggtc tcgcaggacg
     2461 tccagggtgt tacggagccc cgatacggcg atggcccgca cggggcgacg ggcaaagcgg
     2521 cgcacgtgct ccgcctctcc ccctcggagg gcccagagga ggtcccgccg ggaggagagc
     2581 tccgcccggg gggagtgatt ttttatcccc gacgacagca gatcccgcac cactcgacgg
     2641 acggacacgg cggcaccgat ggcgctccgg tctcgcccca ggggctcccg caccatggtg
     2701 atcttggccg ggcagggagc caaaagcacg ggggggcacc ccccggactc tcgggcccag
     2761 agttcggcgg ccacttccag gggagattcc gctggcacgg tacgggatag gagctcggga
     2821 aagcggatgc ggatcaggcg cagcaccgcc ggacagtagg tggagatcag gggaagctcc
     2881 tcccgggggg cctcgtccag gcatcgggcc accgccaggg ccgccagatc aaaggccctc
     2941 cgggagtcca aggtctttat ccccgccatc tccagggcct cgggggcccc atcggggctc
     3001 caggcggcaa actgggcgaa aaagctggga tccgccagca cggggacctg ctgggacagg
     3061 agctcccgcc aatcgtcctc gtcgagcccc agggccttct tacgacaggt cctcaggcac
     3121 tcgccgcagt cgatgcagag gtcggggagg atgtgcacca ggccatcgat gacccgaatg
     3181 gcctcggtgg ggcaggattt gatacagtgc acgcacccct tgcaggcggc gttaaatatt
     3241 tttatcccat gcatcatatc cgtggcctcc ctcttcaggc ggagggctgg ggctctccga
     3301 caaagtagat cgatgccgtg aggatggtgc cctggcccac cgtggactcg atgtcgaggc
     3361 ggtcggcgtt gcgcttgatg ttgggtaacc ccatccccgc cccaaaaccc atttcccgga
     3421 cctggtccga ggcggtgctg tacccctctc gcatggcttg atcaatgtcg gcgatgcccg
     3481 ggccctggtc ttcggtgacg aggaccactt tttcctcgtc gatgcgggcc cggagcatgc
     3541 ctcccccggc gtggatgact agattcattt ccgcttcata ggtaatgatg gccgcccggc
     3601 ggcagatgga agagggcacg ccgagcattt tcagggtgtc tttgacgctg ctggacgcct
     3661 ctcccgccgc gaggaagtcc tccccctcca cggggaaatc cagcaccacg ggatctttca
     3721 atgctcctcc ccccctgcct ctcgatgagg aatgatgcaa gctggcaggc cagcggcaaa
     3781 gagcactccg ctggtctcgt acatgctctt catggtcacc aggaggggaa tgcgagactg
     3841 agccgccagg cggacggtat cctcctgggg gcgtttcccc cggacgaaga ccacggcggg
     3901 gatgtccatc atctgggccg tccggacgat ctggatgttc gtcaggcccg tgatgagcat
     3961 ggaccccgcc cgggcaaagg ccagcacgtc actcatgaga tctgcggcgt agccaaagtc
     4021 cacatccata ttctccgccc actctccccc gcagagaaac tccccttcga ggatcgctcc
     4081 tatttcctgc accttcatca catgaaacct cccatcgagt tcgctccgat cccggcctat
     4141 tcggggatcg tccgggatcg gatgcgcatt tccatcatcg gacgccaaga ttctccatcg
     4201 ctttgaaaca ccgtgaccat ccggcccaag ggacggtgcg tcccgggatc gatgtccgga
     4261 cactcctccc ccagggggat ttttttgctc cgccccaggg cggccaaaag ggcccccccc
     4321 acccctcggg cctcgtccac cccttggaaa aaccaccacg ccagggcgta ggcccgggcc
     4381 gccatggggg gatcggccac ccgaaagcgg gtgcggtcga agatcctgcc ccggaagtcg
     4441 cgcaaggtgg gatcgtgctc cagggcgtag cgctgatccg ccgccagaat gcccggacgg
     4501 cggaccaaat ggccattcag gcttcccaga taccatagcg ccaaggggag gtgttcctct
     4561 cggataaagc gccaggcatc cagggttccc atggtgtcca gggcgctcac cagcaccgag
     4621 gagcccaccg ccacggcctc ccgcaggcgg ctccccaggt ctcgatcccc cgcccccatg
     4681 gcccagaggg gcagcacgga gagctcccga aacaagaggt cttggataaa aagctccgcc
     4741 gtgcccgcca ggtaagggtc aaggcgatcc cccagcaccg tgatccccgc cgccgggtcg
     4801 agcctccggg tggcatattc cgccagggct ctcacgcgaa agcggcggta aaggtcgagg
     4861 gcaaaggcat ggtcgaaggg tgcatctccg ctacgcaacg acaccccttc gccaaaacct
     4921 aataataggg gaaactccgt tcccgcgagt tcctgcacca gcacccgatc gggctcatca
     4981 tcggcgaagc tgaaaaaacc caccaccatg tcctttttcc accggggaag gcgctcccgg
     5041 aggtgagacg ccagatcttc cagggggggc atttcctccc ggaggaactc cacgtcgtga
     5101 ttctgcaagc cgtagcgccg ctcgttcgac tcctccgccg ccatctgaag ggccgcccaa
     5161 atcgacttcc ctgcggggga atcccaccct tcgaaggggg gaaagaccac caccctccag
     5221 agccacccct cggccccggc gtcaggagaa ggcttttttt gcccccgggc ctccggcagg
     5281 ggttctccca ggcctaggcc aagccccaga atcataaaaa acaaaagggc cttgaggaag
     5341 acggccccgc cccaaatccc gggcctccgg cgggaagggc ccccaggcga cggggaatac
     5401 cccgaaagag cccctttcgg acaagggact ccgggatcga cggataaatc cctatcactt
     5461 ttccttcgcg caacgcccct ccgcacgacg ccccttcccc cttttgccct ttttttcaca
     5521 cctccagggt ccattctgag atatgatacc tcacgaaacg aaaggtggga acggggccgg
     5581 ggcctttctc gggaaaatct tcgctttttc gaaaggagca ggtggcatgg aagtcttcgg
     5641 actctcggcg acggcggtca ttttttcctt tgccctgctg ggggtggtga gggggaggaa
     5701 aacctccggg ggagactttg ccctgagcgg gcggagggtc tcttcccggg gggtctcggg
     5761 aatccttctg ggggccctgg tgggaggggc ttccaccgtg ggcaccgccg agatggccta
     5821 cgtccacggc ctttccgcct ggtggttcac cctgggaggg gggctggggt gtcttttggc
     5881 ggggcttttt ttcgcccgcc ccttgcgacg ggcccacatc gtcaccattc ctcagtttct
     5941 gggacaggcc tacggccccg ccaccgctct ggtggcgctg gccggatctt ccttggggac
     6001 gtttctttcg gtgctcgccc aatttctttc gggagaggcc ctgctgcgat cggtcctacc
     6061 cctgggggcg gggggatcgg ccgccgtcct ggggatcctc gtcgccgcct ttctcttcgc
     6121 gggggggctc cggagctaca gcgccgtggg gagcgccaag atcgtctttt tgtacttcac
     6181 cctgatcctc tgcggcggag cggcctttct tcaggggggc acccccgggg ccctgatccg
     6241 cgaccttcct ccctttccct atctgtccct attcagccga ggggtgtctc gggacctggg
     6301 ggcggcggga tccttggtgg tgggagtcct ttccacccag atctacctcc aggccctctt
     6361 cgccgccaaa gacgagaccg tcgcccggcg aggggccctg tggtccgccc tcctcatgcc
     6421 ccccttggga atcctggccc tctgggtggg gctccatctg cgttgggccg gggtggagat
     6481 tcccccctcc caggccctcc cccacttcct gcgcaccgcc tttccccccg ccctgggggg
     6541 ggcgctctgg ggggggattt ttatcaccgt gctgggcagc gccgccggat tggtcttcgg
     6601 catagccacc aacttggttc aagacctgat gattccctgg gcttcccggg acttttccgg
     6661 caaaagcctc ctgtggaccc accggggagc cgtgggagcc gtcctcatgg cggcggccct
     6721 ggtggagctc tccctggaga agtccatgat cctggactgg agctttctca gcatggggct
     6781 ccggggggca ggggccttct ttcccctggt gtgggcggtc cttcgcccgg gagttttgcc
     6841 cggcccctgg gccctggcct cggggctcgg gggccttggg gcggcgctgc tggccccgtt
     6901 ttttcccttt cccgtggaac ccctcttcgc ggggcttacg gtctcgggcc tcctggcgct
     6961 ggggggagtg atcgccgggc gagggcgccc cacgcccctc cggactcccc ccgcctgacc
     7021 tcaaagccgg gccccgaggg cttcgacttt cgcacaaaaa atccgtgagc ccttgccatt
     7081 actagctttc taaaaaaata taggcactac gagatgtctt cctccgggag aacgctcccc
     7141 ctcgtagtcc tcgtttcgcg gcaaggcccg ctggaagatg gccccaccgg caaaagagcg
     7201 cgaaagtcgg aaaaggggct ggcggatcgc cgccagcccc tcgcgccccc tatgccccag
     7261 aggggtcagc gtttgtaccc gatgatctcc cggagaagtt tcttccgact ctcttcgtct
     7321 tcgttcccct ccaccccccg agaagcgaag ccgtccacca cccccaccac gccccgcccc
     7381 tggtccgttt ccaccccgat gacctgcagg gggttggccg tggcggcgtc gatgcgacac
     7441 acctcctgca ccgccttgat gcggtccagg acgttgatgg ggtacccatg gcgcagcaag
     7501 atgatgaaag tgtgtcccga gcgaagctgc atggcgttct gcaccgccgc ctgtaccaga
     7561 tccggggcgt tgccgtcccg gcgaatgagg cagggccccg aggcctcgca aaaggctatg
     7621 ccgaactcca gcgacgggga cgacgtcacc agggcctcgt agaggtcttc cacggtcttg
     7681 atgaaatggc tctgccccaa gattatatta cacccctccg gaatggagag ctgctccaca
     7741 aaccaccctt ttatttctgc caaaagaaac cctcctttca gtgggacgaa aggggtctag
     7801 gccccgccct tcttgcgccg caccccttgc caggcgtgga gcgccagggc caccccgatg
     7861 acgccgtagg ccacaaagac ccggaagaac tcccccacct ggacatttcc caaaaggttc
     7921 ttcccagcca agggggatac gatgaacaaa gtatgaaaaa gaagaatccc catgagggcc
     7981 tgtccgatgg tggccttgga caccgatgcc cctcctatga ggatcgccgc cacggcgaag
     8041 gtccccacct ggacgtggct gccgtaggta ttcatgttgc ccaggttctg caggaagatg
     8101 atctgtccca gggccgccag caccgtggaa aagatgatgg ccaaaacgcg cacccgatcc
     8161 accgggatcc ccgccacttc ggcgatgtgc cggttctgtc ccacggcccg aaaatcctgc
     8221 cccagtttgg tcttaaacag gagcaccatg cccacgcaga gggctacgat gaccagcaaa
     8281 gtgaccacgg ggatggtcac ggggaccccc gatccgaaag agatgctgat cttccagata
     8341 ttgtccaggg cgtagtgtat ctgctttaag tctatggtat ttctcaggcc gatgcctccg
     8401 gggatgagca tactggccac ccggatgggg atgaagttcc ccaggatgta aaggcagatg
     8461 aactgataaa ctccgttggc aaaaaacccc aggatgatcc ccgtgatcat ttccttgccc
     8521 ttggagttgt tgaagagctt ccccgtgagc cacccgaaga ggatggccag ggggatggac
     8581 aggatcaccg tgaggatcag ccctacgaag cccccgatct cccagtttgc cgtggcgata
     8641 aaggcgaact gccccgccat ggcccccagg acgatgccga agttgagccc catccccgcc
     8701 aggatgggga tgatgaggga aattaccagg aagctgttcc gggccatgcg cacgatgatc
     8761 tcgttgatca aaaagggcag cgtcaacccg ctgtagtatg cccccaccac gcagagcacc
     8821 gtgaaaagca agggcaccac gttccggcga agggcttcca tgacgtcaaa tctcttccgg
     8881 ggatctccgg aagaccccaa atgatcctgc tgcggcgtgg tcatcgtaca tccacccccg
     8941 cttccttccg cgtcaacgcg taaaggatga tgccattgct cacgatgatg cggatcacct
     9001 ccgccaagtt atccgccacc accttgttcg ccacaggcat ggagaccacc aggatggcct
     9061 ggaacaggaa ggtcccgatg agcacgtgcc acaccgtggc ccggcggagc gatgcccccc
     9121 cgatgagcac cgacgccacg gcaaagaacg ccataaacag cggggcgagg tagagctgga
     9181 tgaacccgaa gctctgggcg taaacgatga tccccaccgc ccccagggcc tgggagacca
     9241 ccgaaccaat gatgcggtat ttatcgacgt tgagccctgc ggactcggcg tagcggggat
     9301 tgctccccac catcatcatg gcgacgccgg tcttggagcg gaggaaaagg gccaagaggg
     9361 cgcaaaacat cccgaaaaag agcaacagcc ccgtgggcac cgtcatggtg ccgatcttaa
     9421 acgacatgaa gccgtccagc acctgggcaa agcgattcgt gagggtgatg gtgacccgaa
     9481 gccccgaccc cccatagggc cagatcatct cggggctctt gtacggcacc acggtccaga
     9541 agatgcacat gagggatacc acggaaaaac caaaataggt tcccaccgtg agctcctgcc
     9601 ccttcacctt attctggaag acgccgtaga gataccccga gattgccccc aggggaatgg
     9661 ccacccccac ggcgaaaaag aacgccgtaa agcctcgcag gtccagctcg atgctcagcg
     9721 tggacgccac gatgccgcag gtgactccca ggggcagggc gaagttcggc cccgtgccgg
     9781 actggatcga cggaatcatg gcgagcacta ggatgccgtt catcccaaac cgggtgagga
     9841 tatttccgaa aagttgcccc atggggagcc tcaacgatac cgccgtcgcc agaagcaccc
     9901 cgagaaaacc caggatgatg atccggggaa tgccaaaatc ccggaccgcc gcccccaggc
     9961 ggtcgccgaa actctccccc ccctcggggg agggaagcgc attcaggagc atggagattc
    10021 ctcctttgaa ccttggcccg aaccctggcc gcaggaacca gaaagatgcg agggcccttc
    10081 ggcatccctt ccccggtggg atttttctcc cgccatgagg agcccaaaat cgcggtcact
    10141 ggcgtcgggg ggaaggatcc cctccaggcg cccctcacag acgatggcga tgcggtcgca
    10201 gatggagcgc aactccgcca gctccgagga agtcaccacc accgtgacgc cgtgttcgcg
    10261 gttcaggcgg accagaaggt ccagcacgat ctttttggct cccacgtcaa taccccgggt
    10321 gggctccgac acgaaaagga ttcggggctc cagcgtgagg gccttggcaa tgcagacttt
    10381 ctgttggttc ccccccgaga ggcgccggac aatctgctcc ggccccgtgc aacggatgtc
    10441 caactcctcg atgaagcgct cggcgtggcg gcgcatggcc cgacggtcca ttatgctaaa
    10501 ctgaaaaccc cggggcccca ctttacgcag gaacttttcc tgactctgca tggcgggggc
    10561 cgccacgttc atctcgatgg attcttccag gagcaacccc acgccccgac ggtcttcgga
    10621 gacaaaggcc agaccgctgc gaagggcctc cagggtatga cccaggggca ggggagtccc
    10681 ttcgatggcc atgatccccc gcgcgggata catgcccatg acgccgttgg ccaccccgac
    10741 cttgccgtgc cccgccagac ccccgagccc caggatctcc ccctggaaca cctccagcga
    10801 aaggtctttt accatctctc cgggcatatc cacttccagg tgctccagcc ggagcaggac
    10861 ctgatccgtg cggggagagg gggcgcaccc atcgcacccc accgcttcga gctttcgtcc
    10921 caccatgagc tccgccaagt gccccaccga cgtctctcgg ggagagagat cggccacctg
    10981 ctctccatcc cggagcaccg tgatgccgtc ggagacctgg accacctcgt ccaggcggtg
    11041 cgtgatgaag atgagggata tcccctgggc ggcaagggat cgcatggcat tgagcaacac
    11101 ctgagcctcg gactccgtga gcaccgccgt gggctcgtcc agcaccaaga gccgggtctt
    11161 tttcttgtcc aactctcggg cgatctccac aaactgcatg tgccccacgg gaagccccgc
    11221 cacggggagc atctcgtcga tggacatgcc catacggtcc agcgacgccc gagagtcgga
    11281 tcgcatggcg ggcatgtcca ggtgctccag cttaggtccg cataatctgc tcaaaagatt
    11341 gggacgggtc agttcccgat tgagcttgat gttttccgtg atgctaaaac cgggaagcag
    11401 catgaactcc tgatgcacca tgccgatgcc cagttccacc gcctgttggg gagaacgaat
    11461 gctaatcggg gccccgtcga agaggatttc cccctcaaac cccccggtat tgtggatcac
    11521 cggcatgccg aagaggatgt tcatcagagt ggacttgccc gcaccatttt ctcccacgat
    11581 ggagaggatt tcccccggcc ccacccgcag gtttacccct ttgagcaccc ggttcccgaa
    11641 gtactccttg gcgatgcctc gcatttccag acgtggcgta tccaatccgg aaatacctcc
    11701 tttccccaaa aaacgcgggc cgacctcgga gagccggccc gcgcacaccg aggcgcctcg
    11761 aaagatgccc cccgagatcg cccgtgggag agctcgaact agagtccgaa aatcttggac
    11821 tcgccgatga agaggatgaa gttatcggcc ttgtccgcgc cactctggta ggtggaaagc
    11881 ctcacgtcgc ccgcgtactt cttgaagagc tcttccagct tagcccgatc gaatcccttg
    11941 atggacccat cgccgatgcc gaaggcgtat tccgtggcga tgtaggtcat ggccatgttc
    12001 gccggggact tccacgtggc gaagcgtccc gcgccgccca tttccttcac cttggcgtcg
    12061 atggcttcga tgatgaagtc cacatcgccc gccttgtccg ccggaatctc aatgcccagg
    12121 gcgccggggt aggcgtggta ggggctgggg cagcactgtt cggggtaaag agcccccgtg
    12181 gtcatgacgg ccttgatgag gggttcctgc atgccacagt tggtgttgaa gaacgcggtc
    12241 tccttaccgt actgctccac ctgccgcggc acgtcttcca ggataaactg ctgggctccg
    12301 gcgatccccg catctcccgt ggggtcgggg gcgttgacga acacgaactc cagcccctgc
    12361 tttttgcact cctctaccat gatgtcacga cggttcgcca gcaacgggta ggacatgtgc
    12421 cgggggaagg agtagtggat gaaggtcttg gcgcccattt tcttggcgag ctcgacaatg
    12481 gtgacgcccc gggtggggtt atccgtctcc aggacgatgt ccgccttttg ggccgacagc
    12541 atggggtctt cgtgggggac gatgttgatg aagaacatat ccggacggat ttctttcacc
    12601 ttgtcgatgg cagcggcggt gccaggaaca ccctggacca ggacgatggc cttcacgtcg
    12661 ggatcctggg ccatgctgag gatttgctgg atggtggttt cctgctcgtc cataaacttg
    12721 tcggggtagg tgacgtgaac gatgcggtcc gccccgtagt cgttgatcat cttctgggca
    12781 gcccggtact cctcttctcc ctgggacgtg gttcccgtca tgatgcccac cttaggaagc
    12841 cccgcgaaag ccgccccgca gaagcacact accagcgcca taacaagaaa gctcagcata
    12901 ctctttttca tcagtctgac aacctcctca ttctgtccgt tctggaactg gcgatagacc
    12961 aaggaaccgc aaaaacctcc gccgccaccc gcgttttttc gctccgcctc tcccccttcc
    13021 ccttgtccat ctccggggcg atctatcaag aaaaacttca cggacccccg ccagccaatt
    13081 ctaccaaaag ttttccctcc agggaacccc ttgaggtctt ttcccgactt ttcggagccc
    13141 ctttcacgtc gggcatgacc aaaagaggag gtcaaaacaa ggcggggcgt ggagaagacc
    13201 cgccctccgc gccccgccga aaatcccttt cttgatgcct ggcatctttt tcggaacaaa
    13261 agttcttaat tttctcccgt tttaaatacc tggatccatc gggcatcccc gctgatgagc
    13321 atgggcaggc tgatgagcca cgtccggtcc agccccaagg agaacttttc ctcccgggtc
    13381 tggatcacct tgccccctcg gttcattacc tccacccgga tcacgtgatt tttccccacg
    13441 agcatctcgg ggaggcgctc tcggcgctgg atctccacgg ccttcttccc atcgagggaa
    13501 atcagggccg acgcaaaggc ctcgtaggtc tggtccccca tcgtcaccgt cttgttgtcg
    13561 aggaggatgt cgtgcccctt gccgttcacg aacagaaaaa cccccagagc cacgaacaga
    13621 aaaagcgccg ccgcccgcac caccagtctt ctggctttca tgcccttcgc ccccccatcc
    13681 tagtggccgt tcgccggagc gggggcatcg ccgccccgga gggactggcg ttcccgctcc
    13741 ttgtccatgc gccgccgcag ggcgtggagc accagggcca gggcgatgat gccgtaggag
    13801 acgaagaccc ggaaatactc cccgagctgg gcctgcccca tgagggtctt ccccgccatg
    13861 ggcgacacca cgaacatgag gtgaaagagc atgaccccca cgaagacgtt ggtgatggtg
    13921 gcccgagaga ccgtggcccc cccaatgagc agcgaagcaa tggcaaagat cccagcctgc
    13981 tcgtgggcgt tatacgtgtt catggtgccg atgttttgca ggaagatgat ctggccatac
    14041 cccgccagca ccgtggagat gatgatggcg atgatacggg tacggtccac ggcgatgccg
    14101 gcggatttgg ccaccgccat gtcctgcccc acggcgcgca tgtcctgccc cagcttcgtg
    14161 cggttgaacc agagaatgaa gaggcaaaac agggcgatga cgatgaacgc cgccatcggc
    14221 acgtggatag gtccgatgtc caccatgagg aacttgtcaa gaacctgccg gactccctgg
    14281 aggttgatgg cgttacggat gccaaagccc cgggagagga gcatggcgtg attttccacg
    14341 ggaatcacca tgccgaagcc gtacagcaca ataaactgat acactccgtt catgaaaaat
    14401 cccagcatga acgatgttac catttcgcgc cccttggccc gattcaagat ctcccccgtg
    14461 agccatccca gggccagcga tatgggcgtg gaaatcatgg ccgccagcag aagcccctgc
    14521 atccccacga tgttccagtc cgtcaccagg atgatggcga tctgtccccc catggctccc
    14581 aacaccatgc cgaagttgag ccccatgcct gcggcgatgg gaataagaag ggacagcacc
    14641 agaaaacagt tccgccccat gcgggtaacc atttcctgaa tcagatagct ggtggtgaac
    14701 cccgaaaggg ggatggccgc cgcagaaagc accaggaaga tgatgggcac cgcgttggaa
    14761 ataagtatat ctttaaacga tttattcttc atcgcttaac ctgcaccttc ctcgtcaggg
    14821 catagataat catgccattg gagacgatga gccggatcat ctcggacatg tccgtctgga
    14881 gcaagctgtt gatgaccgaa ggcgtcatgg tacagatgcc ctgatacaga aacgtcccca
    14941 cgatgacgtt gaagagggtg gccttgttca ccgacgcccc tcccaggaga agggctgcca
    15001 cagcgggaaa ggccatgtag aagggccccg tgtagagctg aaggaatccg aaactttgct
    15061 ggtagaccac gatgccaatg gccccgagca tggtggaaag caccaccgaa atcgtacgca
    15121 ttttgtccac gtcgatgccc gacgcccggg caaattcggg gttcgatccc acggcggtca
    15181 tggcagtgcc gatcttggtg cggaaaaatc cccagaccag gaaacacatg aaagctaaaa
    15241 agagaaacat ccccgtggga aagtagaagt tccctatctg gaaggacagg aagtcgctca
    15301 gcacatgaag ccagtgctca ctcacgctca ccgtcacccg aaggccgctg cccccgtaac
    15361 cccagatcat gatggggctg gtaaagggca gaagctgcca ggcgatgcac atgagcgaca
    15421 ccgaggaaaa gcccacgtag gtggcgatca tcatctcgtc gcccttgacc ttgttcagga
    15481 tcaacccgta gccatagccg aagatcgccg caaagggaag agaaaccccc atggcgaaaa
    15541 agagccccgc aaaaccggtg atgtccagct cgatgctcag cagagccccc aaaagccccg
    15601 cgatgatccc caggggaaga ccgaagttca gcccgcatcc ggattgcacc atgggcacca
    15661 aagcgagcac catgacgccg ttcatggcca tgcgcaccag cgtatcgctc accgagcgag
    15721 ccacgctgat ctgggccagg ggtgccacgg cgaaaagaag cactagaaaa ccgccgatga
    15781 tgacgcgggg cagtcccgcc gcctcgataa aggctcgaat ttttttcata gccacctcac
    15841 ctcacccccg cggcgacttc cgggcccttg cccgcgccga gcatgagtag gccaaagtct
    15901 tccaccgaag cggtggccga aagcatcccc gccagacgcc cctcgtcgac aatggcgatg
    15961 cgatcgcaga tggaacgcag ttcttccagt tcggaagaga tcatcaccac cgtcgttccc
    16021 ttgtggcggt ttgtctcccg aatggtgtcg agcacgagct tcttggcccc cacgtcgatg
    16081 ccccgggtgg gttcggccac aaagagcagc tcggggccca ccgcgaaggc cttggccaga
    16141 cacacttttt gctgatttcc cccggagagc tccaccgccc tctggcgaga gctcacgcac
    16201 ttgatcccca gggcgtcgat gtactcctgg gccagagacg ccatggcgac gtcgtcgcgc
    16261 cactggatga gcccccccag gaggctcttc aggcaccgcc cctggacctg catggccgta
    16321 aaggcgatgt tccagtcgat gggctcgtca agcaaaagcc ctacgccgcg ccgatcctcg
    16381 gaaacgaagg ccatgcctgc ctccagggct ccgtgggggt cgttgagctt cagggctttt
    16441 cccttccaag tcacggcccc cccggcggca aagagcccca tgatgccatt ggcaatgccc
    16501 agttttcctt gtcccgccaa ccccccgatg ccgaagattt cccctcggca aacgtcaaaa
    16561 gagacgtcgc gcaccgtctc cccgggcata tccacccaga ggtgatccac tcggaggatg
    16621 ggttcgaggc acactctcac ggcatcatcc tgctttttgt cctccacggt tcttcccacc
    16681 atccaggagg cgatctgcct tatctccgtt cccttggtgg gggcctcttt tatcaccatg
    16741 ccatcccgga gcaccaggat cttgtcgcag agatcaagaa tttcctggag gcggtgggag
    16801 atgaagatga tggcgatgcc gctgtcagcc agcttacgaa gggccttgac cagaatggcc
    16861 gcctcgctct cggtgagcac ggcggtgggt tcgtccagga cgagaagttt tgtgctttcc
    16921 cggtcgatct ctcgggcgat ttccgtgaac tgccggtgcc ccacgggcat ctcctggatc
    16981 aacatgtccg gatccacccg cactcccaga gtgtcgatgg ccttggccgc ccgctggcgc
    17041 atggcgggac ggttcaacgt ctggagcctt tcgccgaaga gctccaccag gggggaatag
    17101 caggtaaact cccggttcaa catgatgttt tccgtggtgg aaaagcccgg aataagggag
    17161 aactcctggt gcaccatgcc gatgccccca tccaaggcgt caaaggggga gtcgaagttc
    17221 acctctttgc cgtcaaggta catcttcccc tcgtatcccc ccgtctcttt tatgacgggc
    17281 atgccgaaaa gaatcttcat caaagtggtc ttccccgccc cgttttctcc gacgaggcca
    17341 agaatttccc cccgttgcaa cgtcaggttt acgtccgcca agacacggtt gccaaaatat
    17401 tctttgccga tgtgctccat ccgcaaaagc ggttcttggg tcatcctcgc ttctcactcc
    17461 tttgccgaaa aagtgccgga agggatttat ctctcccgat gtttttcgag cgcttcggat
    17521 ggggaaatcc gaagtctcat ggcgcgctcc gggcgcctca ttaccaaaac aagagggaat
    17581 gggggcggtg ccccccactc cctccttgtg ctgtgctcgt gcagtgtgct cccccgggga
    17641 ctgatattaa aaatcaccgt gcaaacttat gggagatgcc tactgtccgg tgacggtgaa
    17701 gtacttgtcc ggcacgacct cgtcggtcat cttcatgtag cccttgccga agatgtaggt
    17761 atcctggtag agcagggggt agttcttcat ggtaacgccc gtggaagcgt cggtgtagaa
    17821 ggacgcgttc cattcggcgc cgggggagaa cttgccatag gcctcgagga cttccttttt
    17881 gatgtcgatg ttgccgttct tgaagtcttc gggggtcttg ccctcttcca cgagcttctt
    17941 acccatctcg cccatggcca ctcccgtggt gtaggcgaag gagaaagccc aggtgcccat
    18001 gcggcccgca gcgcccttgg ccaccaccgc atcctccacc cgcttcagga tggcgggcca
    18061 gtcaccttgg acggaagaaa gatccacacc aagggctccg ggatagccca acaggggaga
    18121 aggaaggtcg gactcgatga agaagccgcc catctcggcc acgcgcttca gcaggggttc
    18181 ggtctgggcg tcgttggtgc agaagaacgc cgtctccttg ccgtacttgt cgagccacgc
    18241 ggggaacttc tccaggataa actgctgggc gccggggata ccaacgtcgc tggtggggtc
    18301 cggagcggtt tcgaaggcat aggtcatgcc caggtccttg cacgcctctt ccatgatggc
    18361 gcgccggcgg gacaggagct cgatgctcat gtgccggggg aaggaaatgt gcacgaaggt
    18421 cttggcgccg agtttctggg cggccttaac gatgagatag ccccgagcca cgttgtccgg
    18481 atccgtcacg acgtcggcgg cctgggtggt gaggttcggg tcttcctggg acatgccgat
    18541 gagcaggatg atgtccgggc gccgctcgcg gatcttgcgg taggcctcgg tggtgccggg
    18601 gacgccctgg acgctgacga tggccttcat gaggggatcg tcggcaagcc ccacgagctg
    18661 ggcgatgacg gtctccatct cggaggagaa gttgtcgggg taggtgacgt gcttgatcat
    18721 gccgccgttg gccacgtcgc catacatctc aatgagcttt tccgctcccc ggaggtcgtc
    18781 ctcggactgc gagagcgttc ccgtcatgat gccgatgtgg aacggagcat ccgccagcgc
    18841 gccgccagca aaaaacgtca ccagcgcgat caaagccaaa aacacgattc ctcttttcat
    18901 gaatctatcc cctcccaact aaagataggc cccctcgggc accgcacggg cacagcgtac
    18961 tccaggccga atatcttgtt aattctggat tttgtttcat cccactccct ttgggacaaa
    19021 acgttttacc ctccgacccc ctagaggggc gaaggctttg gtaaaggtaa accggcaaag
    19081 acgatgagtt tccctcggag gtcgcggttg atgtttcttt ttgattaatc agtgccgcgc
    19141 aaggatttat cgatggcaac gccaagaagc catggcgtct aggcaaaatt ttttcttgcg
    19201 cttttacctt cttggcgtct gtgtgacacg ccaacgaact acactgaatg ggacttttat
    19261 ctcattttga ttgatcttaa agacccagaa aataggagaa aagacccccg ttcccccctt
    19321 acccattgcg ccccgggcgc gtatcgctat aatagggaca cggtagtaaa ccatttcagg
    19381 aggtggtcgg ttttgaagaa gaaggtaggg tttctggctt gcgcggcgct tttggtgatg
    19441 gccttcgccg gtatggcgat ggctgcggac gacgtgatca aggtgggtta cctcgccgcc
    19501 ctcacgggag actgggcggc ctacggccag accgaggcca actccgcgca gctggcggtg
    19561 gacgagatca acgccaacgg cggagtcctc ggcaagaaga ttgagctggt tgtttccgac
    19621 ttccgcactc gccccgagga cgcggtgaac gccgtgcgcc gcctcgtgca ggaagacaaa
    19681 gtggtcgcca tcatcggcgc caacggcagc ggtatcaaca tcgccacggc ccccatcgtg
    19741 aacgaggcca aggtccccca gatcggcacc gtttccacga accccagcgt caccgtggac
    19801 aaagacggca aggttcgccc ctacagcttc cgcatctgct tcaccgaccc ctaccagggt
    19861 aagctcatcg cctacttcgc cgccaaagag ctcggcaagc tcaagggtgc catcctctac
    19921 gacgtggcca gcgactattc tcaggggctc cgggagttct tcctgaagag ctacgcggag
    19981 tacggcgggg aaatggtggc ggacctgggc ttccagggcg gtaccgatgt ggacttccgg
    20041 gcccagctca ccgagatccg cgataagggc gccgacgtcc tggtgctacc caacatgggt
    20101 aaggaaatgg ctctgaccat gaagcaggcc cgggaacttg gcatgaacga catcatcttc
    20161 gtgggcggag acggctacgg cgagttcatg tgggagatcg cgggagacgc catggagaat
    20221 tcctactgga tcaaccacgt ggcccccgag gaccccgcca tggctccctt cttcgcggac
    20281 tacaagaaga aatataacga cgagtgcaaa gagttcacca acggcatcct ggcctacgat
    20341 gccatgtact ggctcgccga cgccatcaac cgggcgggcg aagtggattc caccaagatc
    20401 gcccaggccc tggagagttc cgtcggcgtt cagctccacc acgccaccct cacggtggac
    20461 cccacggacc acaaccccaa ggacaaggac gccgtggtcc tcgtggccaa agatggcaag
    20521 ggacagttct tcaagaagat caagccggaa taggccccgc accgataatt cctgaattcg
    20581 catccgcgaa aggcgtggcg aaggcgggcg cctaaagggg cgcccgcctt cgctttttcc
    20641 gaaagagggg tggaaaccgt ttgggtatct tcttacagca gatcatcaac ggcctgtcct
    20701 tagggtcggt ctacgccctc atcgccgtgg gctactcttt ggtgtattcc gtgttgcttt
    20761 tctcgaactt cgcccacggg ggattcctcg tggtgggggg ttacgcatgc tacttcgccc
    20821 tgaaaactct agggttcaat atttggattt catctctact ctcgttggtg ggggcgggat
    20881 ttgtcgccat cttggtggag cgtctagcct acaagcccat ccgggagcgc accaatgtca
    20941 ccctttacct tctgatcgcc tccatgggca tgagcatcgt catcgagaac atcttcgtcg
    21001 tcacggtggg aggacgattt cgagccctgc cccccgtcat ccccacggcc ccggtgaacc
    21061 tgggcatcgc caccaccagc gcctttgaca tcatctcctt gggcgtggcg gtggtttgcc
    21121 tcgtggccct ccagatcttc ctatcccgca ccaagtgggg cttggccatc cgggcggcgg
    21181 cctacgatct acgcacggcg gggctcatgg gggtaaacgt caaccgcctt atctccatcg
    21241 tcttcttcgt cgcgggggcc ctggcggcgg tggggggcat cttcctctcc gtgcgctaca
    21301 ccctctaccc ccagttagga accatcacca tcaaggcctt cgtagcggca gtcatcggcg
    21361 ggctgggctc ccttcctggc gccgtggtgg gaagcctcat cctgggtctg tcggagatgc
    21421 tcacggcggg ctttatttcc agccaactcc gggatctggt ggtcttctcc ctgttggtca
    21481 tcactctcct ggtgcgcccc acggggctct tcggcaagag tgtgggcgag aaagtgtaag
    21541 ggaggagccc catgtcccca tacgttgaaa gcatcatcac cctgctcatg atcaacagca
    21601 tcgccgccat gggggtgtct ctgctcaccg gcttcaccgg catcttcacc ctggggcact
    21661 cggcctacat ggccctgggg gcctatacca cggccatcct ccacatgggg cactccgtgc
    21721 cctgggccat cgccatcttc gccggggcgg cctttgccat gctcgtcgcc tggctcatcg
    21781 gcgtccccac cctgaggctc atgggggact actacgccat cgcctccatc ggcctggccg
    21841 aatccatccg gctcatcatc gccaactggc aatccctcac ccggggcgcc cggggatatc
    21901 cggacatatc aaaattcgcc acgttcaacg tgactttagc ctttttcctg gtcatgggag
    21961 cgctcatctt tctcctgatc cacagccgat acggtcgggc ctttaaggcc tgccgcgacg
    22021 accacgtggc cgcctccctt ttgggctaca acaccgcccg gtaccgggtg ctctccctgg
    22081 tgatctccgc cttctactgc ggcatcgccg gagggctctt ggcgggcttt ttgcagtacc
    22141 tcaagcccga tatgtttgac atgctcaagt ccacggagct caccgccgtg gtggtcttcg
    22201 gaggcatggg ctccatgagc ggctgtatcc tgggaactac catcatcacc ctggtgacgg
    22261 aactcttccg ccccatatcc caataccgca tgttgatcta cggcgcggtg ttggtgatca
    22321 tcatggtgct ccgccccgaa ggtcttctgg ggcggtatga gctcgggcct cggctcctgc
    22381 ggcacctctt cggccgcaag acccccgagg gcacggaagg gaggggctag gccatgggcg
    22441 cccttttgga agtaaaggac atgaacaaat tcttcggcgg cgtccaggcg gtggcggaca
    22501 tgagctttac cctggaaaag ggggagctca tggggctcat cggccccaac ggcgcgggga
    22561 aaaccaccat cttcaacctc atcaccggag tgtatcccct gtcgagcggc tccgtgttct
    22621 tccgagggga agacatcacc ggggggaaaa cctacgacat cgttaaaaaa ggcatcgccc
    22681 gcaccttcca gaacctacgg ctcttcgggg cttccacggt gctggagaac gtcatgacgg
    22741 cgtgccagca gcactacccc tactctttcc tggaagccat ctcccatctg gggcgttgga
    22801 aaagccagga aaaaaatatc cgtaataaga gcatggagct tttggagcgg gtggatctgg
    22861 cccgggaagc ccatcgggcg gcgggcaccc taccctacgg ccaccagcgt cgattggaga
    22921 tcgcccgggc cctggccctt cagcccga
//
LOCUS       DAOOER010000031        22287 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_2718_length_22287_cov_9.331684, whole genome shotgun sequence.
ACCESSION   DAOOER010000031 DAOOER010000000
VERSION     DAOOER010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 22287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22287
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2718_length_22287_cov_9.331684"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..2015)
                     /locus_tag="PL162_05625"
     CDS             complement(156..2015)
                     /locus_tag="PL162_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HQF91536.1"
                     /translation="MLPSVSHDPRWRERRCNPQPIRERKREGGPKIARPADMTRGEGN
                     EETSSRRMCPLKGPFWPHIYHHKGVICTMKKFAALLVVAVALAFAAPAIAANPFMDVP
                     MNHWAYDAIGQLAASGVVNGYPDATFKGNQPMTRYEMATAVARALAVVDMQKASKQDV
                     EMLKKLVVEFKDELDALGVKVDNIDSRLAVIEERLGGWKINGDFRFRMEWFDTDNSAR
                     FRLHRARLTFKKYIDENVTFTARLRHVGDTNLTSTRWDLMFIDVKLPWDFTMRMGRQN
                     IDYWDEEGLYADNDALMFDTDLDAVRFDRDFGMGTVSFYVAREMATDGNNGLNNVYLN
                     NTVLDNYYTAGFKARIAPSETWGLQLFGEFFWLNDPLYQALGATPADQALLAAQDDNF
                     HTYAANVYVNFTPNIRADLTYLIQGYGERWRVRMQELGGAQYIEDSSSALRAAVSVGQ
                     EALGFTSLRLEYTKFGRGWLTYMDPYGYYFNVLAAYSAVSEHANVWAGTPLFTAQDGL
                     SGIGNVLPDEVKTLYIEANQKWNDKWSTALRYVTADMDSNFRLFNTLPGQNTASSFYS
                     WKTTDWTLRVQYNYTPSLSFQLTYDKIDYDNGLELFGGQADDSLIRLQTHVSF"
     gene            complement(2185..2826)
                     /locus_tag="PL162_05630"
     CDS             complement(2185..2826)
                     /locus_tag="PL162_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQF91537.1"
                     /translation="MRTANFPLSVDEVHRELLRKFITGQLGPGERLPEIALAAQLGVS
                     RTPVREALRRLAGEGLVVLVPNGGARVVSPTPQELEETFEVRCEMECLAARRAAKRIT
                     PAQLCLMEEQAEEEDRLIRDWDVEAYVRTNTRFHLIMAEASGNSVLGEYLENILARTF
                     AYMVFVQSFLASDRNPSVGEHRAIVEALRARDPDGAEGLIRHHLSRTVGDFRA"
     gene            3229..4299
                     /locus_tag="PL162_05635"
     CDS             3229..4299
                     /locus_tag="PL162_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HQF91538.1"
                     /translation="MSSRIEALRAALRSASLDALFVTRGTNLRYLSAFTGADSFAMIT
                     PSRSFFITDSRYTEQASRECPAFEVVPWRDPFASLPETLHRLVREGGIARLGFEKRFI
                     SYGLYEELASGLPGVSLTPTENVVEDLRYVKDPGELAALRRAAEVADRAFEALLPFLA
                     PGRTERDVAAELGYLLHRAGAEGMAFDPIVASGPHSSMPHAVPSDRALERGDFLTLDF
                     GAQWGGYRSDMTRTVVIGRASARQRELYARVLASQQAGIRALKSGASGRDVDAASREA
                     LAGQEGIFSYGVGHGVGLDIHEEPFMKKTCARRLEPGCVVTVEPGIYLPGEGGIRIED
                     TAMVWEEGPEVITRSTRELLEL"
     gene            4381..6003
                     /locus_tag="PL162_05640"
     CDS             4381..6003
                     /locus_tag="PL162_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HQF91539.1"
                     /translation="MRKEWYKAAAVLWGVMALGWGVGSPALGAQEFVYSVNAEVPGLD
                     PQKSNSVPSMVIGIHVWEGLVRVHDGKILPGMAEKWEISVDGLVYTFHLRDAQWSDGS
                     PVTAQDFVYTFQRLLDPKVAAEYAFAAYGIVNGAKYNKGEIADPSEVGVKALDDKTLQ
                     IVLETPMNYFLGYLQLMCFLPCKKDFVEAAGDAFATEADKMLYNGPFVLKEWKHEEMM
                     VLEKNPNYWNKDAIKLDRVTVHQVYEANTALSMFENGELDMVNIPSNLYAQYQKEGRA
                     LVYMTGADDWIKVNVHPNPEKPWLSNKNFRKALAFAIDREQYLQIATKGLYFPNPRYV
                     LPIMAGLNGKYGDEFPLEFYSKNAEPDKAKEYLQKALEELGIDDPAKITVTYTIQDEE
                     ECRLMAEALQDQLQRALGITVKVQLVTRKQRATLENQREYDLVYSGWAPDYDDPLTYI
                     EIWTGDNPHNNSGFAHDKFDNLVAISRTEVNPQKRLDVLFEAEKILLDEAPMIPLQLR
                     RRAWTAQPYVQGVVRPFIGADLDFVFASVEGK"
     gene            6108..7034
                     /locus_tag="PL162_05645"
     CDS             6108..7034
                     /locus_tag="PL162_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQF91540.1"
                     /translation="MTRYILQRLAISLVTIWVLVTVVFVLVRCLPGDPFASEKLTPEI
                     RANMERYYGFDQPLSVQYAKYLKNLLRGDLGMSLKYQNMPINQIIQNSFPYSADLGIR
                     TILFATFTGIFLGVVAALNRGRPLEYLCIFVAVVGISLPDFITGSLLQYLLGQKLRLF
                     PVALWKGFSYTVLPTFALSFYTLAMVSRIMRTSMLDVTGQDYIRTARAKGLSPFKIVW
                     RHQIRNAILPVVTILGPITAAILTGTFVIESIYAIPGMGKFYVLGVQNLDYTMILSLT
                     VFYGVFLIAANFVVDILYGFIDPRIRLAGNKR"
     gene            7040..7966
                     /locus_tag="PL162_05650"
     CDS             7040..7966
                     /locus_tag="PL162_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017359137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQF91541.1"
                     /translation="MTEPTTSFDDALFSRVERDASALEAIHRPSLTYWQDAWYKIKKN
                     RVAMLSLGIIALCVLMAAVGPSMTPFDYTATDATHIDEPPGGTHWFGTDSLGRDLWAR
                     VWMGARVSLLIGFLAAVINSCIGACVGGIAGYLGGRVDMFFMRLIDVLYAIPYMIVAI
                     LAMVVLDTGMHTLVIALVLVGWIKSARLVRGQVLQLKHQEFVLAARKLGASDARIIYR
                     HLLPNTLGLIITDLTMAVPYAIFAEAFLSYIGLGIQPPEASWGLLARYGAQNFRVCPY
                     QLFIPAACISLTMLSLNLFGDGLRDALDPRVR"
     gene            7972..9030
                     /locus_tag="PL162_05655"
     CDS             7972..9030
                     /locus_tag="PL162_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQF91542.1"
                     /translation="MAPGNAPDRVPGSPPLLEVRDLRVNFATYGGTVQAVRGLSFSME
                     AGECLALVGESGCGKSVTAQSLLRLVPHPPATTTGSVRFDGRELLDLSEREMNRLRGA
                     AFGMVFQDPMTSLNPTMPVGRQIEEALREHERLSRSAARERVLDMLRLVGMPEPERRY
                     GQYPHECSGGMRQRVLIAMALVCGPRLIVADEPTTALDVTIQAQILDILKNLQSRLGT
                     ALLLITHDLGVVAHMAHRIAVVYAGRIVETAPVRELFARPSHPYTRRLLCSRPSLDGE
                     AARGPLCAIKGTPPDLFAPPPGCGFAPRCDYAMALCGTTPPPALGVGTGHRSSCWLLH
                     PQARERYGDLMRAPLERR"
     gene            9033..9995
                     /locus_tag="PL162_05660"
     CDS             9033..9995
                     /locus_tag="PL162_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQF91543.1"
                     /translation="MTATPLLEARNVSKHFVLGGGAVLAAVDGVSLALHRGETLGLVG
                     ESGCGKSTVGRTLAGLYDPTGGEVLFDGKNITALSSHERQDFTRSTQMIFQDPYASLN
                     PRMTVGDIVAEGLDIYRICRGKERMHRIYELLSLVGLHKEHANRYPHEFSGGQRQRIG
                     IARSLAVEPRVLVCDEPISALDVSIQAQVVNLLLGLQERFGLTYLFISHDLSMVRYLS
                     DRVAVMYLGSLVETAESDELYATPLHPYTRALLSAIPLPDPEKEGSRRRILLEGDVPS
                     PINPPPGCRFASRCPEGLPLCAEVRPELRECAPGHFLACHLHGR"
     gene            complement(10053..11252)
                     /gene="preA"
                     /locus_tag="PL162_05665"
     CDS             complement(10053..11252)
                     /gene="preA"
                     /locus_tag="PL162_05665"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF006183.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HQF91544.1"
                     /translation="MEATLETTFLGKKLKNPFMLASAPPTRSREMIERAFDAGWGGAI
                     IKTLTQKEHPARTNVTPRIRALRQGSQIWGFTNMELASMHATEAWMADLASLKARYPD
                     RGLGASMLYGGAPDEAQWRAVARECEKAGVDWLELNLSCPHGGAEEGGEFSIGCRPDA
                     IRTVVGWVRESTRLPLLVKLPAFSDIEAGVRASRNAGADGIALINTLNALSGIDLDTY
                     RPLPSVAGKSAFGGLSGRAVKPVALRCVTLAAGASPLPVSGMGGIYDWKDAVEFLLAG
                     ASTLQICSAVMEHGYGIIDDLCRGLLTYLEVQGWHSPGDAVGRALPHLVPHADLSREA
                     RGVASCQQERCLRCGACAVSCRDSGYQAISWAPGETPRVDQEACDGCGLCVGICPVGC
                     LELKARG"
     gene            complement(11418..12122)
                     /locus_tag="PL162_05670"
     CDS             complement(11418..12122)
                     /locus_tag="PL162_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQF91545.1"
                     /translation="MLSVRDLHVHYGGIHALQGISLEVPLGKVVTLIGANGAGKSSTL
                     RAIAGLVRQKSGAILWKDRDLIHSSADDIVKSGIAMSPEGRRIFPQLTVRENLLLGAY
                     IRNDAAEVENDLEWVYTLFPRMRERSWQKGGTLSGGEQQMLALGRALMSRPDLVMLDE
                     PSLGLAPLLVKEVFDIIRTVNERGKTVLLVEQNAYAALKVAHYAYVLEVGNIVLQGTG
                     EELIADPRVREAYLGG"
     gene            complement(12115..12903)
                     /locus_tag="PL162_05675"
     CDS             complement(12115..12903)
                     /locus_tag="PL162_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQF91546.1"
                     /translation="MTERVSVLETKNLVMRFGGLTAVNDFSISVPEGSIVGLIGPNGA
                     GKTTAFNMITGFYRPTEGQVIFQGQDITGLPPHEVCRAGIARTFQNIRLFGNESVLQN
                     VMIACQVRQKLPWWHAPLFLPGYFREEKEIREKSLALLDAVNLRSHADERAPSLPYGA
                     QRRLEIARALATEPKFLLLDEPAAGMNPQESIELMDFIRSIRQRFNLTILLIEHDMKV
                     VMGVCQYIWVLDYGVRIAEGTPEDIRSNPKVIEAYLGEEALEHA"
     gene            complement(12900..13943)
                     /locus_tag="PL162_05680"
     CDS             complement(12900..13943)
                     /locus_tag="PL162_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQF91547.1"
                     /translation="MNRTRDTILSLLLLGALGAFLWWADGHLDKYQIQVLNVVAINII
                     LAVSLNLIYGFTGLFSLGHAGFMLVGAYVCAILILPASQKAATFILQPMMWPFSVMNA
                     PFIVAVLAGGVVAAVFGFLIAFPVLRLGGDYLGIASLGFAEILRVVANNIVPITNGAL
                     GLKGIPKEANLWWNYGWCAVTLVVIARLINSNAGNVLKAIRDDEVAAKAMGIDVFRWK
                     LISFTLGAFFAGVGGALLGSLLTTISPKMFTFLLTFNVLMIVVAGGLGSITGTVISGV
                     FITILMEWLRFVEAPMHIGTMTIPGIPGMRMVVFSLALLCIILFRREGIMGMREWSWS
                     GLFGLFGKRGEAR"
     gene            complement(13943..14836)
                     /locus_tag="PL162_05685"
     CDS             complement(13943..14836)
                     /locus_tag="PL162_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQF91548.1"
                     /translation="MSLEMFVQHLCNATTLGSLYALTAIGYTMVYGILRLINFAHGDI
                     FMLGAYFVFFGGALFALPWGVAVIGAIILSTACGIMVDRVAYRPLRDAPRISALISSI
                     GVSFLIENLAIVIFTAIPRPVHQPEWLSQVFLVGGVRILSLTLVVPAISFLLVAGLLW
                     IVYKTKPGLAMRAISKDIETTRLMGVSVDKIIALTFGLGSALAAASGIMWALRYPQIN
                     PLMGIFPGFKAFIAAVLGGIGSIQGALIGGLLLGFIEILVVAFLPALSGYKDAFAFVL
                     LIVILLIKPTGLMGEKLEEKV"
     gene            complement(14934..16064)
                     /locus_tag="PL162_05690"
     CDS             complement(14934..16064)
                     /locus_tag="PL162_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQF91549.1"
                     /translation="MKLGKWALAALLVCFVASAAFAADDVIKIGVYLPLTGQNAFGGQ
                     LELDGVNLAHSLVSDVLGKKVELVVLDNKGDTTECANAMQRLVEQEKVNAVIGTYGSS
                     CAMAGGEINEKAGVPAMGTSCTNPLVTQNKKYYFRACFIDPFQGKAVAQYAFDNGKKK
                     AALLLDVAQDYSVGLGKFFKDHFTKIGGEVVAELNYQSGDQDFTAQITEIISKGADIL
                     FIPSYFAEGAIIIKQARDAGAEFLIIGADAMDNPEMVKIGGKAIEGFTFTTFPYAIEM
                     PDMSDTQKAFTEAWQKQFPDKEPNANSALGYDSYMLVIDAIKRAGSADPEAITTALAA
                     TKDFDGVTGKTTINETHDAEKAVGIKMVKDGKITFVTTVLPQ"
     gene            complement(16261..17451)
                     /locus_tag="PL162_05695"
     CDS             complement(16261..17451)
                     /locus_tag="PL162_05695"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HQF91550.1"
                     /translation="MAMAVPMAFVKEELDQLKKEGRFTHIRTLESPQGAWVIIEGKRR
                     LNLCSNNYLGFCNDPRLCAGVKEAIDRYGVGPGAVRTIAGTMRIHKDLEERLAAFKGA
                     EAAIVVSSGFTANMTAIPPVVGKDDLVFSDELNHASIIDACRLSRATIIRYGHNNMED
                     LEARLREHASHPGRKLVISDGVFSMDGDVAPLPAIVDLAEKYGAMTAVDDAHGEGVLG
                     RSGRGIVDHFHLHGRVDLEIGTLSKAFGVMGGFVTARAEVVEFLRQKARPNLFSSALT
                     VPDTAANLAAVAILEQSDEPVRKLWENGRYFKDRMKAQGFDTGKSETPITPVIVGDSN
                     LAKKFSARLFDEGVFATAIAFPTVPMGTARIRAMVSAAHSRDDLDQGVEIFARVGREL
                     KIIP"
     gene            complement(17576..17755)
                     /gene="rpsU"
                     /locus_tag="PL162_05700"
     CDS             complement(17576..17755)
                     /gene="rpsU"
                     /locus_tag="PL162_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HQF91551.1"
                     /translation="MTQIVRRDNESIDDALRRFKREVQKVGVLREARKHEHYEKPSEV
                     KKKKRAAAKRAPRTY"
     gene            complement(17790..18125)
                     /locus_tag="PL162_05705"
     CDS             complement(17790..18125)
                     /locus_tag="PL162_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HQF91552.1"
                     /translation="MARCLFCDIVERHGADFVFQDDAVVAFRDIHPQAPGHILIVPRV
                     HVPSAAALDDPGLWGRVMSAAVKVAREEGFAEQGYRLVVNCGEQACQTIPHLHVHLLG
                     GRSFRWPPG"
     gene            complement(18143..19168)
                     /locus_tag="PL162_05710"
     CDS             complement(18143..19168)
                     /locus_tag="PL162_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HQF91553.1"
                     /translation="MNLEREMSFRTLGLSPDASWDDVKSAFRRLALACHPDVAGPASA
                     RRFQEITQAYMTLRQTIEGDPGGAGARRAPSAPPRKTGMFARVLGGWWTWWRSRRIRR
                     EESRAEGRRQAWEETEKRRRRQTLVDGILDEAELRFQDVLRRSVVGGREDAVELHLER
                     LSSGHPVVRCMALDALMERWDHPAVVPGLAQALAEGVFSGETLRRLLGSRMPKNATTA
                     LAQAMKSRVALLDEGDALFALRWLRGAGDAGWETLEASLFHPAPAVVGDALLQWSRLA
                     PLPKDQTIKHLLGREEERVLVPLLRLMKGRSASRDVVDLLVKLSQHTSPAVRAWARAL
                     VSEAEAS"
     gene            complement(19165..20748)
                     /locus_tag="PL162_05715"
     CDS             complement(19165..20748)
                     /locus_tag="PL162_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HQF91554.1"
                     /translation="MSSSETPVLGIDLGTRYAVGALCWGDSSPVVVSHRWGGERTPSY
                     VAWHEGRWITGEDAFRLSLKEPGRAWWNVKRFVGSSSWRVTVEGRTFAAEDLLVPLLA
                     TLREDAEAFAGTFLTSCVLAVPAHFGFPERAAMARTARGAGFDQVRIVNEPTAAALAT
                     GVEGRSLVLDFGAGTTDVTVVERDGAVWHVVESQGRGDLGGVDLDRALADHLWRSTFS
                     GRLREDDPRRALLMNDAERIKIALSDSRRTPWYPPGGLGAPPEGSVDVSREAFEALAR
                     RLLLEVLSMVRRLWSRHNPHHLVLVGGGSRIPLLRQMLAEQVAEPARFRTPPDEAVAL
                     GTALYACQSGQRLLIDVLSASLGVEAADGNVVTLLERGTPLPCAASRRFASVKGGGVS
                     VPVVEASGRARRRLLGTVRVSDVGEGDEVEVRFEVDGGGVLHVHVKLRGMVVRHEVLD
                     VAPGASPAAEDRDLAELERRFSRLSLHLSPEQQERVASLLWRARSLGGEAADVLARDD
                     ALGILSELAHELERAVQRP"
     gene            complement(20745..22058)
                     /locus_tag="PL162_05720"
     CDS             complement(20745..22058)
                     /locus_tag="PL162_05720"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HQF91555.1"
                     /translation="MSATLEGRTVRMEVLGCRTNQYEGDALAFALTEAGARLVDAPPY
                     DVALLVSCTVTAVADRKGRALVRRFRRECPDAVVALCGCQAQVMAEGEARGLGVDLLV
                     GNRRKARLPELLARRLAQGKGGGGVELCRGDGESDPWDPLELGRPMRHTRAFLKVQDG
                     CDHGCAYCIVPSVRGRPLSRPPEEVWAEARRVVASGCAEVVITGVHLGLYGRDGACSL
                     GELVRRLGEIEGLRRIRFGSLEPFALSEGLLEELRGVAAFCPHLHIPLQSGDDGVLRR
                     MRRGYVASDFSRQVEALRRAFGDEVHVSTDLLVGFPGETEEAFRRTLDLVTQLGLGRL
                     HVFPYSPRPGTAAALWTDRPSREEARRRCREADALGKELLGRYAARFVGQEVDLLVEE
                     GSEEAVQGLSPHFLRVRGEGPGDPGSLMTVRVRGEDRGVLWGDRG"
     gene            complement(22055..>22287)
                     /locus_tag="PL162_05725"
     CDS             complement(22055..>22287)
                     /locus_tag="PL162_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HQF91556.1"
                     /translation="FAAFLSGDARPLPGEVFGDEVALAIGPEGDFSPEEGEALRRKAF
                     LPISLGGRVLRAPTAAAVGCAWFTVFHEGGAP"
BASE COUNT         4038 a         7276 c         7022 g         3951 t
ORIGIN      
        1 tgggtgggtg ggtgggtggg tggaaggaaa ggtggggcga ggcatcggtg gacggcccgg
       61 ggactcccgg gggtcgcccc ggaggcggcg gagaggagga gaaaaaaggc aacaaaaaag
      121 gcggggtttc ccccgccttg tctggagccg cgatcttaga agctgacgtg ggtctggagc
      181 cggatcaggc tgtcgtccgc ctggccaccg aagagctcaa ggccgttgtc gtagtcgatc
      241 ttgtcgtagg tgagctggaa gctcagggaa ggcgtgtagt tatactgcac ccgcagagtc
      301 cagtcggtgg tcttccagct gtagaagctg ctggcggtgt tctggccagg gagagtgttg
      361 aagagccgga agttggaatc catgtcggcg gtgacataac gcagagcggt ggaccacttg
      421 tcgttccact tctggttggc ctcaatgtag agggtcttca cctcgtcggg aaggacgttg
      481 ccgatgccgc tgagaccatc ctgggcggtg aagagaggag taccagccca cacgttggcg
      541 tgctcgctca ccgcagagta agctgcgagg acgttgaagt agtacccgta gggatccatg
      601 taggtgagcc agccccggcc gaacttcgtg tactcgagcc gcagggaggt gaagcccagg
      661 gcctcctgac ccacgctgac ggctgcccgc agggcgctgg aggaatcctc aatgtactga
      721 gcgccaccga gctcctgcat ccgcacgcgc cagcgctcgc cataaccctg gatgaggtag
      781 gtgaggtcgg cccggatgtt gggggtgaag ttgacataga cgttggcagc gtaggtgtgg
      841 aagttgtcat cctgggccgc cagcagggcc tggtcggcag gagtggcgcc aagcgcctgg
      901 tagagaggat cgttcagcca gaagaactcg ccgaagagct gcaggcccca ggtttcgctc
      961 ggggcgatcc gggccttgaa gcccgccgtg tagtaattgt ccagaacggt attgttcaga
     1021 tacacgttgt tcaggccatt gttaccatcc gtagccattt cccgggccac gtagaagctg
     1081 acggtcccca tgccgaagtc gcgatcaaag cgcaccgcgt ccagatcggt gtcgaacatc
     1141 agggcatcgt tgtcagcgta gaggccttcc tcatcccagt agtcgatgtt ctgacggccc
     1201 atccgcatgg tgaagtccca gggaagcttc acgtcgatga acatgagatc ccaccgggtg
     1261 ctcgtcaggt tcgtgtcgcc cacatgacgg agccgggcgg tgaaggtcac gttctcatcg
     1321 atgtacttct tgaaggtgag gcgagcgcgg tgcaggcgga agcgagcgga gttgtcggtg
     1381 tcaaaccact ccatgcggaa gcggaaatcg ccgttgatct tccacccgcc gaggcgctcc
     1441 tcgatgacgg cgaggcggct gtcgatgttg tcgaccttca cgccgagggc gtcgagctcg
     1501 tctttgaact ccaccacgag cttcttcagc atctcgacat cctgcttgct ggccttctgc
     1561 atgtcgacca cggcgagagc ccgggcgacg gcggtggcca tttcgtaacg ggtcatgggc
     1621 tggttgccct tgaaggtggc atcgggatag ccgttgacga cgccggaggc ggcgagctga
     1681 ccgatggcat cataggccca gtggttcatg ggcacgtcca tgaagggatt ggccgcgatg
     1741 gccggggcgg cgaaagccag cgccacggcg actaccaaca gagctgcaaa tttcttcata
     1801 gtacaaataa cccccttgtg atgatatatg tgcggccaaa aagggccttt cagggggcac
     1861 atccgtctgg aggaagtctc ctcgtttcct tctcctcggg tcatgtccgc tggccgggcg
     1921 atctttggtc cgccttcccg tttcctctcc cgtattggct gggggttgca cctcctttct
     1981 cgccatcggg ggtcgtggga aacgcttgga agcattgtca aggtgttcgt gggaccctaa
     2041 aagatcctgg acagatggta ctacaggaaa tggcggagag tccatgggtc ttgagaccca
     2101 tcttttcttt tcgccgaacg aaagggccgc cccgcccccc ggggaggccc caccgccgac
     2161 gcctgaaggg gccccggggg ggcctcaggc gcggaaatcc cccacggtgc gggataggtg
     2221 gtgccggatg aggccttcgg ccccatcggg atcccgggcc cgcagggcct ccacgatggc
     2281 ccggtgctcc cccacgctgg ggtttcggtc ggaggccaga aaggactgga cgaagaccat
     2341 gtaggcgaag gttcgggcca agatgttttc caggtactcc cccagcacgg agttgccgct
     2401 ggcctcggcc atgatgaggt gaaatcgggt gttggtgcgg acgtaggcct ccacgtccca
     2461 gtcgcggatg agacggtctt cttcctcggc ctgctcctcc atgaggcaga gctgggccgg
     2521 ggtgatgcgc ttcgccgccc ggcgagccgc caagcactcc atctcgcagc gcacctcaaa
     2581 agtctcctcc agctcctggg gcgtggggga gaccacccgg gcccccccgt tgggcaccag
     2641 caccaccaat ccctcccccg ccaggcgccg cagggcctcg cgcaccgggg tacggctcac
     2701 ccccagctgg gccgccaggg cgatctccgg caggcgctct ccgggcccca gttgccccgt
     2761 gatgaactta cggagcagtt ctctgtggac ctcgtccacg ctcaggggaa aattcgctgt
     2821 ccgcacgcct ggtccctcct taggatttct taaagggcgc cccgggaagg ggcgaagccc
     2881 cacccatgcg cccgaggtcc cctcgaagga cccgcccctt ccgccgcttc ggggcgcctt
     2941 ttttcgggaa aacacgcccc tcgaggggga cgcccccctt aggggaagaa tgaatacatt
     3001 gaatgctatg cccttttccc cattatgtca atgtccgccc atcgccctcc gcccggggat
     3061 cggcggcaag gccccgggcc ccgggcagag ggcgatgggc ccgacttctg gcttgacagg
     3121 ggggaagatc cgtgccatga tggatgcgat gaattctttg aatacatctc atacaatgct
     3181 tcggcccttg gtcgcaactc actttttcga tattttgagg tgatgcccgt gtcctcccgc
     3241 atcgaagccc ttcgcgccgc cctaaggtcc gcctccctgg acgccctctt tgtcacccgg
     3301 ggaacgaacc tccgctacct gagcgctttc accggcgccg attcctttgc catgatcacc
     3361 ccttcccgga gctttttcat caccgactct cgctacacgg agcaggcttc tcgggaatgt
     3421 cccgcctttg aggtagtgcc ctggcgggat cccttcgcgt ccttgcccga aacgctccac
     3481 cgtctggtgc gggagggggg aatcgcccgc ctgggtttcg aaaagcgttt tatctcctac
     3541 ggcctttacg aggaactggc gtcgggcctt ccgggggtct ctctgactcc cacggagaac
     3601 gtcgtggagg acctccgcta cgtcaaggac cccggggagc tcgcggccct gcgccgggcc
     3661 gccgaggtgg ccgaccgggc cttcgaggcc ctccttccct ttctggcccc cgggcgcacg
     3721 gagcgcgatg tggcggcgga gctggggtac ctcctgcacc gggcgggggc cgagggcatg
     3781 gccttcgatc ccatcgtggc ctcggggccc cattcctcca tgcctcatgc ggtgccctcc
     3841 gaccgggcgc tggagcgggg cgattttctc accctggact ttggggccca gtggggggga
     3901 taccgatcgg acatgacccg cacggtggtc atcgggcggg ccagcgcccg tcagcgggag
     3961 ctctacgccc gggtgttagc ttcccagcag gcggggatcc gggcgctaaa aagcggcgcc
     4021 tccgggaggg atgtggacgc cgcttctcgc gaggccctgg cggggcaaga ggggattttt
     4081 tcctacgggg tggggcacgg ggtggggctc gatatccacg aggagccttt tatgaaaaaa
     4141 acttgtgcgc gacggctgga gcccggctgc gtcgtgacgg tggaaccggg gatctacctt
     4201 ccgggagaag gggggattcg catcgaagac accgccatgg tgtgggaaga ggggcccgag
     4261 gtgatcaccc gatcgacccg ggagctgttg gagctctaag gggaggcccc gccgcgaggg
     4321 gggcgccacg ggatgccccg gctcggccgg ggaggatggg gcaagaaagg agacagggcg
     4381 atgcggaaag aatggtacaa agcggcggcg gtgctttggg gagtgatggc tctgggatgg
     4441 ggggtcggga gccccgccct gggggcccag gagttcgtct attccgtaaa cgccgaggtg
     4501 ccggggctcg acccccagaa gtcgaactcc gtgccctcca tggtcatcgg catccacgtc
     4561 tgggaggggc tcgtgcgggt ccacgacggg aagatcctgc cgggcatggc ggaaaaatgg
     4621 gagatctccg tcgacggtct ggtgtacacc ttccaccttc gggacgccca gtggagcgat
     4681 ggctctccgg tgacggccca ggactttgtc tacaccttcc agcggctgct ggaccccaag
     4741 gtggcggcgg agtacgcctt cgcggcctat ggcatcgtga atggcgccaa gtacaacaag
     4801 ggggagatcg ccgacccctc cgaggtgggg gtcaaggccc tggacgacaa gaccctgcag
     4861 atcgtcctgg aaacccccat gaactacttc ctggggtatc tccagctcat gtgcttcctc
     4921 ccctgcaaga aggacttcgt ggaagcggcg ggggatgcct tcgccaccga ggcggataag
     4981 atgctctaca acggcccctt cgtgctgaag gagtggaagc acgaggagat gatggtcctg
     5041 gagaaaaacc cgaattactg gaacaaggat gccatcaagc tcgaccgcgt gacggtgcac
     5101 caggtctacg aggccaatac ggccctgagt atgttcgaaa atggcgagct ggacatggtg
     5161 aacatccctt cgaacctcta cgcccagtac cagaaggaag ggcgggccct ggtctacatg
     5221 acgggggccg acgactggat caaggtcaac gtgcacccca atccggaaaa accctggctc
     5281 tccaacaaga acttccgcaa ggccctggcc tttgccatcg atcgggagca gtacctccag
     5341 atcgccacaa aggggctcta cttccccaac ccccgttacg tgctgcccat catggcgggg
     5401 ctgaacggga agtacggcga tgaattcccc ctggagttct actccaagaa cgccgaaccg
     5461 gacaaggcca aggagtactt gcagaaggcc ctggaagagc tgggcatcga cgatccggcc
     5521 aagatcacgg tgacttacac gatccaggac gaggaagagt gccgcctcat ggcggaggcc
     5581 ctccaggatc aactccagcg cgccctggga atcacggtaa aagtgcagct agtcacgaga
     5641 aaacagcgag cgacgctgga aaaccagagg gagtacgacc tggtgtactc gggctgggcc
     5701 ccggactacg atgatcccct cacctacatc gagatctgga cgggggacaa cccgcacaat
     5761 aacagtggtt tcgcccatga caagttcgac aatctggtgg ccatatcccg gacggaggtg
     5821 aacccccaga agcgcctgga cgtcctcttc gaggcggaaa agatcctgct ggatgaggct
     5881 cccatgatcc ccctccagct ccggcggcgc gcctggaccg cccagcccta cgtccagggc
     5941 gtggtgcgcc ccttcatcgg cgccgacctg gacttcgtct tcgcctcggt ggaagggaaa
     6001 tagcccttta gagtagcgac cgccgtcccc ggaggggtca cgaaagaaga gccctttctt
     6061 cgtgacccct cgtcctatct caacgcatgg aaaggcggtg aatgggtatg acgcgctata
     6121 tcctgcagcg gctggccatc tccctggtga ccatatgggt gctcgtcacg gtggtctttg
     6181 tcctggtgcg gtgtttgccg ggagatccct tcgccagcga aaagctcacc ccggagatcc
     6241 gggcgaacat ggagcgctac tacggcttcg accaaccgct ctccgtccag tacgcgaagt
     6301 acctgaaaaa ccttctccga ggagatctgg gcatgtccct caagtatcaa aacatgccca
     6361 tcaatcagat cattcagaac tcctttccct actccgccga cctgggtata cggacgatcc
     6421 tcttcgccac gttcacgggg atctttctcg gagtggtggc ggccctgaac cgggggcgcc
     6481 ccttggaata tctctgcatt ttcgtggcgg tggtggggat ttctttgcca gatttcatca
     6541 cgggctctct gctccagtac cttctggggc aaaagctccg gctctttccc gtggccctct
     6601 ggaagggctt ttcctacacg gtgctcccca ccttcgccct gagtttctat accctcgcca
     6661 tggtatcccg gatcatgcgc accagcatgc tcgatgtcac tgggcaggac tacatccgca
     6721 ccgcccgggc caaggggctc tctccgttca agatcgtctg gcggcaccag atccgcaacg
     6781 ccatcctgcc ggtagtgacc atcctggggc ccatcaccgc cgccattctt acggggacct
     6841 ttgtcatcga gtccatctac gccattccgg gcatggggaa gttctacgtg ctgggggtgc
     6901 aaaatctgga ctacaccatg atcctaagcc ttacggtgtt ctacggggtc tttctcatcg
     6961 ccgccaactt cgtggtggat attctctatg gcttcatcga cccgcgcatc cggctggcgg
     7021 gaaacaagag gtgaccagcg tgaccgaacc gacaacgagc ttcgacgacg ccctgttctc
     7081 ccgcgtggag cgcgacgcct ccgccctgga ggccatccat cgccccagtc tcacctactg
     7141 gcaggatgcc tggtacaaga tcaaaaaaaa tagggtggcc atgctatccc tggggatcat
     7201 cgccctgtgc gtccttatgg cggcggtggg gccctcgatg acgccctttg attacaccgc
     7261 cacggatgcc acccacatcg acgaaccccc cggggggacg cactggttcg gcacggactc
     7321 cctggggcgg gatctttggg cccgggtctg gatgggggcc cgggtttctt tgctcatcgg
     7381 gttcctcgcg gcggtgatca actcctgcat cggcgcctgt gtggggggga tcgcgggcta
     7441 cctgggagga cgggtggaca tgtttttcat gcggctcatc gacgtgctct acgccattcc
     7501 ctacatgatc gtggccattc tggccatggt ggtgctggat acggggatgc acaccttggt
     7561 catcgccctg gtgttggtgg gatggataaa atcggcccgg ttggtgcggg gtcaggtgct
     7621 ccagctgaag caccaggagt tcgtgctggc ggcccgaaag ctgggagcca gcgatgcccg
     7681 catcatctac cgccacctgc tgcccaacac cctggggctt atcatcaccg atctcaccat
     7741 ggccgtgccc tacgccatct tcgccgaggc cttcctgagc tacatcggcc tgggcatcca
     7801 gccccccgag gcaagctggg gactcctggc ccgctacggc gcccagaact tccgggtgtg
     7861 cccctaccag cttttcatcc ctgcggcgtg catcagtctc accatgctgt cgctcaacct
     7921 cttcggggac gggctgcgag atgccctgga tccccgggtg cggtgaaggc catggccccg
     7981 ggaaacgcgc ccgacagggt cccgggatcg ccgcccctgc tggaggtgcg agatctgcgg
     8041 gtaaacttcg ccacctacgg cggcacggtc caggcggtgc ggggtctttc gttttccatg
     8101 gaagcggggg agtgcctggc cctggtggga gaatcggggt gcggcaagag cgtcacggcc
     8161 cagagcctgc tccgcctggt gccccacccc cctgccacca cgacggggag cgtgcgcttc
     8221 gatgggcggg agcttctgga tctttcggag cgggagatga accgccttcg gggcgccgct
     8281 tttggcatgg tcttccaaga ccctatgacg agcctgaatc ccaccatgcc cgtgggacgc
     8341 cagatcgagg aggccctgcg ggagcacgag cgtctctccc gttcggcggc ccgggaacgg
     8401 gtcctggaca tgctgcgcct ggtgggtatg ccggagccgg agcggcgcta cggccaatac
     8461 ccccacgagt gcagcggggg catgcgccag cgggtgctca tcgccatggc cctggtgtgt
     8521 ggcccccgcc tcatcgtggc ggacgagccc accaccgccc tggatgtcac catccaggcc
     8581 caaatcctgg atattttgaa aaatttacaa agccgcttgg ggacggccct gttgctcatc
     8641 acccacgacc tgggggtggt ggcccatatg gcccaccgca tcgccgtggt ctatgcgggg
     8701 cgcatcgtgg agaccgcccc ggtacgggag ctcttcgccc gcccctcgca cccctacacc
     8761 cgacggctcc tctgctctcg cccttcgcta gacggcgagg cggcccgggg gcccctctgc
     8821 gccatcaaag gcacccctcc ggatctcttc gcccctccgc cggggtgcgg cttcgccccc
     8881 cggtgcgact acgccatggc cctctgcggc accacgcccc ctcccgccct gggggtgggg
     8941 accgggcatc ggtcctcgtg ctggctgctg cacccccagg cccgggagcg ctacggggat
     9001 ctcatgaggg cccccctgga gaggagatag ccatgaccgc gacgcctttg ctggaagccc
     9061 ggaacgtttc caaacacttt gttctggggg gtggggcggt gctcgccgcc gtggacgggg
     9121 tgtctctggc cctgcaccgg ggagagaccc tggggctggt gggagagtcg ggatgcggca
     9181 aatccaccgt ggggcgcacc ctggcggggc tctatgaccc cacgggagga gaggtgctct
     9241 tcgatgggaa aaacatcacc gccctttcct cccacgagcg acaagacttc acccgcagca
     9301 cccagatgat ctttcaggac ccctacgcct ctctcaaccc ccgcatgacc gtgggagata
     9361 ttgtggcgga gggactggac atctaccgca tctgtcgggg caaggagcgc atgcaccgca
     9421 tctacgaact gctctccctg gtggggctcc acaaggagca cgccaaccgc tatccccacg
     9481 agttctccgg agggcagcgc cagcgcatcg gcatcgcccg atccctggcg gtggagcccc
     9541 gggtgttggt gtgcgacgag cccatttccg ccctggacgt atccatccag gcccaggtgg
     9601 tgaacctgct gctggggctc caggagcgct tcggcctgac ctatctgttc atctcccacg
     9661 atctttccat ggtgcgctat ctttccgacc gggtggcggt gatgtacctg ggatccctgg
     9721 tggagacggc cgaaagcgac gagctctacg ccacgccgct ccacccctac acccgggccc
     9781 ttctgtcggc cattcccctg cccgacccgg agaaggaggg ctctaggagg cgaattttgc
     9841 tggaaggaga tgtgcccagc cccataaatc cccctccggg gtgtcgtttt gcctcccgct
     9901 gtcccgaggg gttgcctctc tgtgccgagg tgcgccccga gctccgagaa tgcgccccgg
     9961 ggcacttcct tgcctgccat cttcacggcc ggtgagggct ttggccccgg agagcgtggc
    10021 tgtggctccc accggcgggt cctaatgccg ctttaccccc gggctttgag ctccaggcac
    10081 cccaccgggc aaatccccac gcagaggccg catccgtcgc aagcctcctg atccacccgg
    10141 ggagtttctc ccggagccca ggagatcgcc tgatagccgc tgtctcgaca gctgacggcg
    10201 caggcgccgc accggagaca gcgctcctgc tgacacgacg ccaccccccg ggcttcccgg
    10261 gagagatcgg cgtggggaac gaggtggggc agcgccctcc ccaccgcgtc gccgggggag
    10321 tgccatccct ggacttccag gtacgtcagg aggcctcgac agaggtcgtc gatgatgccg
    10381 tagccgtgtt ccatgacggc ggaacagatc tggagcgtgg aggccccggc cagaagaaat
    10441 tcgaccgcat ccttccagtc gtagatgcct cccatgccgg acacgggaag gggcgaagcc
    10501 cccgcagcaa gggtcacaca gcgcaaggct acgggtttca ccgcccggcc cgagagccct
    10561 ccgaaggcgc ttttccctgc cacggacggc aagggccggt aggtatccag gtctatgccg
    10621 ctcagggcgt tcagggtgtt tattagggca atgccgtcgg cccccgcatt ccgggaggcc
    10681 cgcacccccg cttcgatgtc gctgaaggcg gggagtttca ccaagagggg caggcgggtg
    10741 gattcccgca cccaccccac tacggtgcgg atggcgtcag gacgacagcc gatgctgaac
    10801 tctcctccct cctcggcgcc gccgtggggg caggaaagat tgagttccag ccagtccacc
    10861 ccggcctttt cgcattcccg agccacggcg cgccactggg cctcgtccgg cgctccccca
    10921 tagagcatgc tcgccccaag gccccgatcg ggatagcgcg ccttgagcga cgccaaatcg
    10981 gccatccagg cttcggtggc atgcatggag gccagctcca tgttggtgaa accccaaatc
    11041 tgggacccct gccgcagcgc ccggatccgg ggagtgacat tggtccgggc ggggtgctcc
    11101 ttctgggtga gggttttgat gatggctcct ccccaccctg cgtcaaaggc ccgctcgatc
    11161 atttcccggc tcctcgtggg gggcgccgag gccagcataa agggattttt cagttttttt
    11221 cccaaaaacg tggtttccag cgtggcttcc atggtatccc tcctccagaa atcgacggag
    11281 cgagcatagc agaaaagcct ttgcggcaaa atgggagcgc ccgaaagttt cgggcgcgcc
    11341 aaaaggcgta gggcccgacg gagatgtcgg gccctacgtt ttgatgcgac atgggttcgg
    11401 acgttcggag agcgcgctta cccccccagg taggcttccc gcacccgggg gtcggcgatg
    11461 agctcttcgc cggtgccctg gagcacgatg ttccccacct ccaggacgta ggcgtagtga
    11521 gccaccttta gggccgcgta ggcgttttgt tccaccagga gcaccgtttt cccccgctcg
    11581 ttgacggtgc ggatgatgtc aaagacttct ttgaccagaa ggggcgccag tcccagggag
    11641 ggttcgtcca gcatgacgag gtcggggcga ctcatgaggg cccggcccag ggccaacatc
    11701 tgctgctctc cccccgacag ggtgcctcct ttttgccagg agcgttcccg catgcgggga
    11761 aagagggtat aaacccactc cagatcattt tccacctcgg cggcgtcatt tcggatgtag
    11821 gcccccagca ggaggttttc tcgcacggtg agctggggaa agatccgccg cccttcaggg
    11881 gacatggcga tgccactttt tacgatgtca tcggcggagc tgtggataag gtctcgatcc
    11941 ttccagagga tggcgccgct cttttgacgt accaggcccg cgatggcccg gagggtgctg
    12001 ctctttcccg ccccgttggc gccgatgagg gtcaccactt tccccagggg cacttccaga
    12061 gagatgccct ggagggcgtg gatgcctccg tagtgcacgt gcaggtcgcg aacgctaagc
    12121 atgttccagg gcctcctctc ccagataggc ctcaatgacc ttggggttgg agcgaatgtc
    12181 ctcgggggtc ccctcggcga tgcgcacccc gtaatccaag acccagatgt actgacagac
    12241 ccccatgacg actttcatgt cgtgctcgat gagaagaatg gtcaggttga agcgctggcg
    12301 gatggatcga atgaaatcca tgagttcaat ggattcctgg ggattcattc ccgctgcggg
    12361 ctcgtccagc aggaggaact tgggctccgt ggccagggcc cgggctattt ccagcctccg
    12421 ctgggcgccg tagggcaggg agggggcccg ctcgtccgca tgcgacctca aattcaccgc
    12481 atcgagcaac gcgagggact tctctcgaat ttctttttct tcccggaagt acccgggaag
    12541 aaaaaggggg gcgtgccacc agggaagctt ttggcgcacc tggcaggcga tcatgacgtt
    12601 ttgaagcacg gactcgttgc cgaagaggcg gatgttttgg aacgtccggg cgatccccgc
    12661 tcggcagacc tcgtgggggg gtagaccggt gatgtcttgg ccctgaaaga tcacttgccc
    12721 ctcggtgggg cggtaaaacc ccgtgatcat gttgaaggcc gtggtcttgc ccgccccgtt
    12781 ggggccgatg agccccacga tgctcccttc ggggacggag atggaaaagt cgttcaccgc
    12841 cgtgaggccc ccaaagcgca tgacgaggtt tttcgtttcc agcaccgaaa cgcgctccgt
    12901 catcgggcct caccccgctt tccgaagagg ccgaagaggc cgctccagct ccactcccgc
    12961 atgcccatga tcccctcccg tcggaagagg atgatgcaaa gtagcgccag ggagaacacc
    13021 accatgcgca tccccgggat gcccggaatg gtcatggtcc cgatgtgcat gggggcctcc
    13081 acgaaacgga gccactccat gaggatggtg atgaagacgc cggaaatcac cgtgccggta
    13141 atggacccca atccccccgc caccacgatc atgaggacgt taaaggtgag gagaaaggtg
    13201 aacatcttgg ggctaatggt ggtgaggagg cttcccagaa gggctccgcc aacccccgca
    13261 aaaaaggccc ccagggtgaa ggatatgagc ttccaccgga atacgtcgat acccatggcc
    13321 ttggcggcca cttcgtcgtc ccggatggcc ttgaggacgt tccccgcgtt gctgttgatg
    13381 agccgggcga tgaccaccaa ggtgaccgcg caccacccgt agttccacca gaggttggcc
    13441 tccttgggga tgcccttgag ccccagggcg ccgttggtga tggggacgat gttattcgcc
    13501 accactcgca ggatctcggc aaatcccaaa gacgcgatgc ccaggtagtc tccgcccagg
    13561 cgcagcacgg gaaaggcgat gagaaagcca aagactgccg ccaccacccc tccggccaaa
    13621 accgccacga tgaagggagc gttcatcacc gaaaagggcc acatcatggg ctggagaatg
    13681 aaggtggccg ctttctgaga cgcaggaagg atgaggatgg cgcagacgta ggcccccacc
    13741 aacatgaacc ccgcgtgccc cagggaaaaa agtccggtga agccgtagat gaggttgagg
    13801 ctcaccgcca agatgatgtt gatggccacg acgttaagca cctgaatctg gtacttgtcc
    13861 aggtgaccgt cggcccacca gaggaaggcg ccgagggcgc ccagcaataa aagcgacaaa
    13921 atcgtgtctc gcgtgcggtt catcagacct tctcctccag cttctcgccc atcaaccccg
    13981 tgggtttgat gagcaggatg acgatgagca gcacgaaggc gaaggcgtcc ttgtaacccg
    14041 aaagggcggg gaggaacgcc accaccagta tctctatgaa gcccaggagc aagccaccga
    14101 tgagggcccc ttgaatggag ccgataccgc cgaggacggc ggcgatgaag gccttgaatc
    14161 ccgggaatat ccccatcagg gggttgattt ggggatatct tagggcccac atgatccccg
    14221 aggccgccgc cagggcggat cccaggccga aggtcagggc gatgatcttg tccacggaga
    14281 cccccatgag gcgggtggtt tcgatgtctt tggatatggc ccgcatggcg aggccgggtt
    14341 tggtcttgta gacgatccat aggagtccgg ccaccaggag aaaggaaatg gcgggcacca
    14401 ccagcgtcaa gctgaggatg cgcacccccc ccacgaggaa gacctgggag agccactcgg
    14461 gctggtgcac ggggcggggg atggcggtga agatgacgat ggccaggttt tcaatgagga
    14521 aggagacgcc gatggagctg atgagggcgg aaatccgagg ggcatcccgc aggggacggt
    14581 aggccacccg gtccaccatg atgccacagg cggtggatag gatgatggcc ccgatgaccg
    14641 ccactcccca ggggagggca aagagggccc cgccgaagaa gacgaaatag gcccccaaca
    14701 tgaaaatatc tccgtgggcg aagttgatga ggcgtaagat gccgtacacc atggtgtagc
    14761 cgatggccgt aagggcgtaa aggctcccca gggtggtggc attgcacagg tgctggacaa
    14821 acatttccag gctcatgaaa tgcccccttc aaggcgaaag acgccccagg ggggagttta
    14881 tcccccctgg gcgccgcagt taacgagtat atccggacaa accgaaggga aggctactgg
    14941 ggcagaacgg tggtgacgaa cgtgatcttg ccgtctttca ccatcttgat ccccacggcc
    15001 ttttcggcgt cgtgggtctc gttgatggtg gtcttgccgg tgacgccgtc gaagtccttc
    15061 gtggccgcca aggcggtggt gatggcttcg ggatcggcgg aacccgcgcg cttgatggcg
    15121 tcgatgacca gcatgtagga gtcatatccc agggcggagt tggcgttggg ttccttgtcg
    15181 gggaactgct tctgccaggc ttcggtgaag gccttctggg tgtcggacat atcgggcatc
    15241 tcgatggcgt aggggaaggt ggtgaaggtg aagccctcga tggccttgcc gccgatcttc
    15301 accatttcgg ggttgtccat ggcgtcggcg ccgatgatga ggaactccgc ccccgcatcc
    15361 cgggcctgct tgatgatgat ggcgccctcg gcgaagtagg aggggatgaa gaggatgtcc
    15421 gcgcccttgc tgatgatctc ggtgatctga gcggtgaagt cctggtcgcc ggactggtag
    15481 ttcagctcgg ccaccacttc gccgccgatc ttcgtgaagt ggtctttgaa gaacttgccg
    15541 aggcccacgc tgtagtcctg ggccacgtca agcaggaggg ccgccttctt cttgccgttg
    15601 tcgaaggcgt actgggccac ggccttcccc tggaaggggt cgatgaagca ggcccggaag
    15661 tagtacttct tgttctgggt caccagggga ttcgtgcagg aagtccccat ggcgggcact
    15721 cccgcctttt cgttgatctc gccccccgcc atggcgcagg aagagccgta ggtgccgatg
    15781 acggcgttga ccttttcctg ctccaccaga cgctgcatgg cgttggcaca ttccgtggtg
    15841 tcccccttgt tgtcgaggac caccagctcc accttcttgc ccagaacatc ggaaaccaga
    15901 gagtgcgcca ggtttacgcc gtcgagctcc aactgaccgc cgaaggcgtt ctgaccggtg
    15961 aggggaagat acacaccgat cttgatgaca tcgtccgccg caaaagccgc agaagccacg
    16021 aagcatacca acaacgccgc caatgcccac ttgcccaact tcatgagtac ccctccttac
    16081 aagagttcgg ccgaagccgt ggcccccatt atacggcaga gagccctttc tgagaacgcc
    16141 ccgaggggaa aatacccccc ggggggcgag ttttttcttg gggccttact tcgccctccg
    16201 ggggggtctc gcggaggccc catcaccaat acgccccccc atccgacggg gacaggggcc
    16261 ctaggggatg atcttcagct cccgccccac tcgggcgaag atttccactc cctggtccag
    16321 gtcgtcccgg ctgtgggccg cagacaccat ggcccggatg cgggcggtgc ccatgggcac
    16381 cgtgggaaag gcgatggcgg tggcaaagac cccttcgtcg aagagtcggg cgctgaattt
    16441 cttggccagg ttactgtctc ccacgatgac gggggtgatg ggggtctcgc tcttgcccgt
    16501 gtcgaagccc tgggccttca tgcgatcctt gaagtagcgc ccgttctccc agagtttgcg
    16561 caccggttcg tcgctttgtt ccaggatggc caccgccgcc aggttggccg ccgtgtcggg
    16621 gacggtgagg gcgctggaga agaggttggg acgggccttc tggcgcagga actccaccac
    16681 ttcggcccgg gcggtgacga agccccccat gacgccaaag gccttggaga gggtgccgat
    16741 ctccaggtcc acccggccgt ggaggtggaa gtggtccacg atgccccgcc ccgatctccc
    16801 caggactcct tcgccgtggg catcgtccac ggcggtcatg gcgccatact tttccgccag
    16861 atcgacgatg gcgggcaagg gggccacgtc tccatccatg gaaaagactc cgtcggagat
    16921 gacgagcttt cgaccggggt gggaggcatg ctcccgcaag cgggcctcca gatcttccat
    16981 gttgttgtgg ccgtagcgga tgatggtggc ccgggagagg cggcaggcgt cgatgatgga
    17041 ggcgtggttg agctcgtcgc tgaagaccag gtcgtccttt cccaccacgg ggggaatggc
    17101 ggtcatgttc gccgtgaagc cggacgacac cacgatggcc gcctcggctc ccttgaaggc
    17161 cgccaggcgc tcttccaagt ctttgtgaat tctcatggtg cccgcgatgg tgcgcaccgc
    17221 cccgggaccc accccatagc gatctatggc ttccttgacc ccggcgcaaa ggcgcggatc
    17281 gttgcaaaac cccaggtagt tgttggagca gaggtttagt ctgcgcttcc cctcaatgat
    17341 cacccaggct ccctgggggc tttccagggt cctgatatgg gtgaagcgcc cctctttttt
    17401 gagctgatcc agctcttctt tgacaaaagc catcggcacg gccatggcca tccctccctt
    17461 ttctctggat aaaagattta tccttcgctc cgtaaagggc cgccttcaca aaaaatggag
    17521 gaggccctcg ccttcctcca ctttttctca agacaaggcg cactccctcc tgaaactagt
    17581 acgtccgggg ggcccttttt gccgcagcgc gcttcttctt cttgacttcg ctcggtttct
    17641 cgtagtgctc gtgcttgcgc gcctcgcgca ggacgcccac cttctgcacc tcgcgcttaa
    17701 agcgccgcag ggcgtcatcg atggactcat tgtcacggcg aacgatctgt gtcatgctcg
    17761 ttccctccct tctcaatcac cgcaccgcat caccccggag gccaacgaaa ggaacgccct
    17821 cccagcagat gcacgtgcag gtggggaatg gtctgacacg cctgttcgcc gcagttgacg
    17881 accagacggt aaccctgctc cgcgaaaccc tcttcgcggg caaccttcac cgccgccgac
    17941 atgacccgcc cccagagccc ggggtcgtcc agcgccgccg ccgacggcac atgaacccgg
    18001 gggactatca ggatgtgccc gggagcctgg ggatggatgt cccgaaaggc caccaccgcg
    18061 tcgtcctgaa agacgaagtc cgccccatga cgctccacga tgtcgcaaaa aaggcaccta
    18121 gccacgacga caccccccta ttctagcttg cctccgcctc ggagacaagg gcccgagccc
    18181 aggcccgcac cgccggagat gtatgttggg acaattttac caagagatcc accacgtccc
    18241 tgctcgcaga gcggcccttc atgagcctca gcaaggggac gagcactcgc tcttcttccc
    18301 gccccagcag gtgtttaatg gtctgatcct tcggcagggg ggccaggcgg gaccattgga
    18361 gcagggcgtc tcccaccacc gccggggccg ggtgaaagag gctggcctcc agggtttccc
    18421 accccgcgtc ccccgccccc cggagccacc gaagggcgaa aagggcgtct ccctcgtcca
    18481 ggagcgccac gcggctcttc atggcctgag ccagagccgt cgtcgcgttc tttggcatac
    18541 ggctccccaa gaggcgccgc agagtctcgc cggagaacac cccctccgcc agggcctggg
    18601 ccaggccggg caccacggcg ggatggtccc aacgctccat cagggcgtcc agggccatgc
    18661 atcggaccac ggggtggccg ctggagaggc gttccaagtg cagctccacc gcgtcctccc
    18721 gcccgcccac cacggagcgc cgcaagacgt cctgaaatcg aagctccgcc tcgtccagga
    18781 tgccatcgac cagggtctgg cgacggcgac gtttttccgt ctcctcccag gcctgacgac
    18841 gcccttcggc ccgggattcc tcccggcgaa tccggcgcga tcgccaccag gtccaccacc
    18901 cgcccaagac ccgggcgaac atccccgtct tcctcggcgg agcagaaggg gccctgcggg
    18961 cgcccgcccc tccggggtcc ccttcgatgg tctgcctgag ggtcatgtac gcctgggtaa
    19021 tctcctgaaa acggcgggcc gaagcgggcc ccgccacgtc ggggtgacag gccagggcca
    19081 agcgcctgaa ggccgacttc acatcgtccc aggaggcgtc ggggctcagt cccagggtgc
    19141 gaaagctcat ttcccgctcc aagttcatgg tcgctgcacc gccctctcca gttcgtgggc
    19201 caactcagac aatataccta gggcgtcgtc ccgcgccagg acatccgccg cctcaccccc
    19261 aagggaccgg gcccgccaca ggaggctggc cacccgctcc tgctgctccg gggaaagatg
    19321 gagagacaat cgggaaaaac gccgctccaa ctcggccaga tcgcgatcct cggcggcggg
    19381 agaggccccc ggggccacgt cgaggacctc gtggcgcacc accatgcccc tgagtttaac
    19441 gtgaacgtgg agcacccctc cgccatctac ttcgaagcgg acctccacct cgtccccttc
    19501 gcccacatcc gagacccgca ccgtgcccaa gagcctccgc cgggcccttc ccgaagcctc
    19561 caccacggga accgacaccc cgccgccttt caccgaagca aagcggcggg aagccgcaca
    19621 gggcagagga gtccctcgtt ccaacaaagt caccacgttc ccgtccgccg cctccacccc
    19681 caggctggca gagaggacgt caatgagaag cctttggccg ctttgacagg cgtacaaggc
    19741 cgtccccaac gccacggcct cgtcgggggg ggtgcgaaaa cgcgcgggct ccgccacctg
    19801 ttccgccaac atctggcgca ggagggggat acggctccct ccccccacca gcaccaggtg
    19861 atgggggttg tggcgactcc aaaggcgccg caccatggag aggacctcca ggagcaggcg
    19921 acgggccagg gcttcgaagg cctcccggga aacgtccacg ctcccttccg gaggagctcc
    19981 caaccctccc gggggatacc acggcgtgcg ccgcgagtcc gacagggcga tcttgattcg
    20041 ctccgcatcg ttcatgagaa gggcccgccg ggggtcgtct tcccgaagtc tgccggaaaa
    20101 ggtcgacctc cagagatggt ccgccagggc ccgatccagg tccactcccc ccaggtcccc
    20161 cctcccttgg gactccacca cgtgccacac cgctccgtcc cgctccacca cggtgacgtc
    20221 ggtggtgccc gccccgaagt ccagcactag ggagcgcccc tccacccccg tggccagggc
    20281 cgccgccgtg ggttcgttga cgatgcgcac ctgatcgaac cctgcccccc gggccgtccg
    20341 ggccatggcc gcccgctcgg ggaagccaaa gtgggcgggc accgccagca cgcaggaggt
    20401 caaaaaggtc cccgcgaagg cctccgcatc ctcccggagg gtggcaagca gggggacgag
    20461 gagatcctcg gcggcaaagg tccgcccctc caccgtcact cgccaggaag aggatcccac
    20521 gaaacgcttc acgttccacc aggcccgccc cggctccttc agggaaaggc gaaaggcatc
    20581 ctccccggtg atccagcgcc cctcgtgcca cgccacatag gagggggtcc gctccccccc
    20641 ccatcgatgg gacaccacca cgggggagga atctccccaa cagagggccc ccacggcgta
    20701 gcgcgtcccc aggtcaatgc ccagcacggg ggtttccgac gaactcaccc ccgatccccc
    20761 cacaagaccc ctcggtcttc ccctcgaacc cgcacggtca tcagggaccc gggatctccg
    20821 gggccctctc ccctcacccg gagaaaatgg ggggataggc cctggacggc ctcttccgat
    20881 ccttcttcca ccagaaggtc cacctcctgc cccacgaaac gcgccgcata gcgccctaaa
    20941 agctctttcc ccagggcatc ggcttcgcga caacgcctcc gggcctcttc ccgggagggg
    21001 cgatcggtcc acagggccgc cgccgttcct gggcggggag aatagggaaa gacgtgaagg
    21061 cgccccagcc ccaactgggt caccaggtcc aacgtccgcc ggaaggcctc ttccgtctcg
    21121 ccgggaaatc ccaccaaaag gtcggtgctc acgtggacct cgtcgccaaa ggcgcgccgc
    21181 agggcctcca cctgtcggga aaaatccgac gccacatagc ctcgccgcat gcggcggagt
    21241 actccgtcgt cgccgctctg gagcgggatg tgcagatggg ggcaaaaggc cgccacgccg
    21301 cgaagttctt ccaaaagccc ttccgacagg gcaaagggct ccagggagcc gaagcgaatt
    21361 ctcctgagtc cttcgatctc tcccagccgc cgcaccaatt cccccaagga gcaggccccg
    21421 tcccggccgt aaagtcccag gtgcaccccg gtgatcacca cctctgcgca ccccgaggcc
    21481 acgacgcgac gggcttcggc ccaaacttcc tccggagggc ggctcagggg acgccctcgc
    21541 accgagggga cgatgcagta ggcacagccg tgatcgcatc cgtcttggac cttgagaaag
    21601 gcccgggtat ggcgcatcgg acgccccagc tccaggggat cccagggatc gctctcccca
    21661 tccccccgac aaagctccac cccgccccct ccctttccct gggccaggcg ccgggccaaa
    21721 agttcgggaa ggcgggcctt gcggcggttg cccaccaaca ggtccacccc caggccccgc
    21781 gcctctccct cggccatgac ctgggcttgg cacccgcaga gggccaccac ggcgtcgggg
    21841 cactcccgac gaaagcgacg caccaaggcc cgtcccttgc gatccgccac ggcggtgacg
    21901 gtgcaagaca cgagcaacgc cacatcgtag gggggggcgt ccaccagccg agcccccgcc
    21961 tccgtcaggg caaaggccag agcatcccct tcgtactgat tggtgcgaca ccccaacact
    22021 tccatgcgga cggtgcgtcc ctccagggtg gcgctcatgg cgctcctcct tcatgaaaca
    22081 cggtgaacca ggcgcacccc accgccgccg ccgtgggggc ccggagcacc cgccccccca
    22141 gggaaatggg gaggaaggct ttgcggcgca gcgcctctcc ttcctcgggg gagaagtccc
    22201 cttcgggacc gatggccagg gccacctcgt ctccgaagac ttccccgggg agggggcggg
    22261 catcacccga aaggaacgcc gcaaagc
//
LOCUS       DAOOER010000032        22037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_2763_length_22037_cov_8.101674, whole genome shotgun sequence.
ACCESSION   DAOOER010000032 DAOOER010000000
VERSION     DAOOER010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 22037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22037
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2763_length_22037_cov_8.101674"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(175..1551)
                     /gene="mnmE"
                     /locus_tag="PL162_05730"
     CDS             complement(175..1551)
                     /gene="mnmE"
                     /locus_tag="PL162_05730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HQF91557.1"
                     /translation="MADPIAALATAPGEGGIAIVRLSGDGVRHMTSLRFSPAGQTPAG
                     EIPPRRMAYGAFLDKEGTVLDRILWVWFAAPRSYTGEEVVELHCHGGYLGAQRLLLEV
                     LDLGCRPAAPGEFTQRAFLNGRLDLAQAEGVLGLIRARNDEALHAAQRALGGGWGWAL
                     LPLRGELLGMAATVQASLDFPDEDLPPWEREDLVGRLKKIRERLQEAQRQARSGALLS
                     TGWRVALVGRPNVGKSSLLNALLQQSRAIVTALPGTTRDVVEEVLSYRGVPFRLLDTA
                     GIRPAQDPAEREGVARALHAAEEADLRLWVLDASEPLLPEDWELGKSLREKGRLLAVR
                     NKSDLPERWDEGALISLLGLEAPVITISAQQERGITALKDLLWHMAVGKDSLEGALHA
                     SARQCQELRQAELAVARAEEALDPGYGEDLLAQGLQEALQALDRLLGFTWDDALLNEI
                     FSRFCVGK"
     gene            complement(1566..3047)
                     /locus_tag="PL162_05735"
     CDS             complement(1566..3047)
                     /locus_tag="PL162_05735"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HQF91558.1"
                     /translation="MKSVPWTNQRSGEACAVLLFEGELCHSSSLGSMASSFQRQCEMG
                     KFSGKKDQMLRLFFPDSQFSQLFAVGMGPRDKATPETWRRAAAEAVRGARRAEVRELV
                     LLAPQAQEPLVAQALAEGALLANDPTRTYQTAKKDKESSVDSPAEKNGTDPLETILLA
                     DGHAEGLGRGTVLAESQIFTRALANEPGNVINPQTLAERAQALAQEEGLTCEVWDEAQ
                     IQQKGLEALWSVGKGSATPPRLIHLSYVPKEGKPRKKIALVGKGLTFDSGGLSLKPAD
                     SMRTMKGDKSGACAVLGALRGIARLKPDIEVHAFIGAAENMPDGKSYRPDDILRTYKG
                     KTIEVDNTDAEGRITLADTLALASEIAPDAMVDIATLTGACGVALGDYTAGLFSSDDR
                     LAEALQIASRETGERVWRLPLDDEMLKKKIKSSVADLLNSGGRQGGAITAAMFLQEFV
                     PPEISWAHIDIAAVDFYKEPFGYYPKGASGFGTRLLLSYLLAQ"
     gene            complement(3078..4286)
                     /locus_tag="PL162_05740"
     CDS             complement(3078..4286)
                     /locus_tag="PL162_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HQF91559.1"
                     /translation="MSELWNTLFSDAALSIRPSPIREMLHMVRRPGMISFAGGMPDPE
                     IFPVENFAASAEILRTQGKDVLQYGTTEGYPPLKEFLRQWTAPRMGRQVEDNEILITA
                     GSSQVVDLLSSALLNRGDCVIIEEPTFLGTMLNMYNHGADFICIPCDAGGMRVDLLPE
                     KIEEARRQGKKIKFIYTIVNFHNPLGCTMSLERRKALLEVAHRYRIPVLEDDPYGYVR
                     FDGEDLPSLFSMDKENMVVYAGSFSKILAPGTRVGWCTGPAEILRKMAIFKQGVDVCA
                     SVVAQAVVYEYCRRGHLDSFLPKIIAHYRKKRDDMEAAFRRHLPMEEVSPWVTPEGGF
                     FYWLETPRIEARPLFEKAIERCVAFVLGEAFYPKGGGTHNFRMCYTFASSEQTEEGCK
                     RLGEAMRSML"
     gene            complement(5243..5677)
                     /locus_tag="PL162_05745"
     CDS             complement(5243..5677)
                     /locus_tag="PL162_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQF91560.1"
                     /translation="MKIRPAAWITRSDRVLMLRYAYPGGTLFSLPGGGQEEGENLEDT
                     LRRELREELAMTVEVGPLIWAGETSPMHHLPATLHILFRCAPGEAPPRLQPHHTKAQE
                     ILWVPLEDIPRLTLYPAVGPWVALFDTQGAAYARVLPPRPWL"
     gene            6391..7425
                     /locus_tag="PL162_05750"
     CDS             6391..7425
                     /locus_tag="PL162_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQF91561.1"
                     /translation="MADQQVFSIAQVRDLSEGGVFWGKFVATSITQKLDKNQKPYWDV
                     AVMDGDGTLEAKVWSNSEWYDRRVEEEEPRRVDPSQDAFFSRISGCTVGVQGKVSSYN
                     GRPQYTFSKVYLLNQEKNPPHAYVRKSPIPFVDLERRFRELVDRAEDPVKGFLQHVFR
                     GERWEKFSTYPAATSHHHAYVHGLLEHTVAVASSALAIAEDYIIRDFSLDRSVVLGGS
                     LLHDLGKVDAYGLDPMPMLTLSGSVHDHIALGYAAFSRLAEEFSLAPTVALHLGHILL
                     SHHGAKEYGSPVLPATAEALVVAAADELDFRLFVYDHTVRSMGDVLGISDYHQATGRR
                     FWKWQETPSA"
     gene            7401..8351
                     /locus_tag="PL162_05755"
     CDS             7401..8351
                     /locus_tag="PL162_05755"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HQF91562.1"
                     /translation="MARDAFRVESHDEGRRLDRILRLRWPEIPLGALARALRTGEVRV
                     DGKKRAGGDPVIAGETITAPWLPPGLPPEREGKALPLEILWEAPELWVVNKPAGLLTQ
                     PGRDAPDSVAARAWAREKGGGAFRPTPVHRLDRNTSGVLLVARSGVGLRALQEAWRLG
                     RVEKTYWAVLWTGDEKIPEGATVDFPLRKDPSTNTVTVAPDGAFSRTIFRTMIRRPPL
                     ALVALDLETGRPHQARVHAAAWGYPVWGDRKYGVITPLPGAGAPPRPLLHARELGLPS
                     LPAPLEGLSGRTFVAPAPEDFVHFFGPLDGAPRRVAKYTR"
     gene            8561..8695
                     /locus_tag="PL162_05760"
     CDS             8561..8695
                     /locus_tag="PL162_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91563.1"
                     /translation="MAKDGVKIWVPAKLRTEGGAVRVGHARFLWKITLYVENALLERA
                     "
     gene            complement(8795..10099)
                     /gene="asnS"
                     /locus_tag="PL162_05765"
     CDS             complement(8795..10099)
                     /gene="asnS"
                     /locus_tag="PL162_05765"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HQF91564.1"
                     /translation="MAAPWVEIRRVADFENREVTIKGWLFNKRSSGKIHFLQLRDGTG
                     FIQGVMVKQAVPEEVFELAKTLWLEASLEVTGVVRADERAPSGFELTVTGLRPIQNPS
                     EEYPIGKKDHGIDFLMDHRHLWLRSRGQHAVMRVRDQVIWSARSFLRDRGFLLVDSPI
                     LTGAVGEGASGLFDLDYFDTGKAFLAQTGQLYAEAAAAAYGKVYCFGPTFRAEKSKTR
                     RHLTEFWMIEPEVAFYDHHDNMKLQEDLVSAIVRDVLENCVPQLTILERDLEPLRKVV
                     APFYHISYDEAVDKLRSLGSDIQHGEDFGNDDETLLTQQYDKPLFVECYPKKVKAFYM
                     KEHPDRSDLVLCDDLLAPEGYGEIIGGSQREDDLDRLLTRIREQGLPEESYSWYLDLR
                     RYGSFVHSGFGMGVERVVAWICGLQHIREAIPWPRTIYRIHP"
     gene            10190..11572
                     /locus_tag="PL162_05770"
     CDS             10190..11572
                     /locus_tag="PL162_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91565.1"
                     /translation="MRRHRLFLGDERKVGSIPLAVLFAAALVERGYALRLFVAGIDET
                     TVRMLQLLCRQPVTVLDPYLAGGDDVMRAIFQCASSDGALNLIIGPLLAKGSNEETVT
                     VDRGSLRVLKALETPVMPILYADMSAVLAARRCEQVFSQISEGAPQSRQMGVCFASVL
                     NPREYQLLEIELGRRIPLISMGFLPSHVHRDIPGEDVLFTADSHSMKITSLRSAVVQV
                     RHMEDQIIWPLVEAMGQMAPEWPEAPFPCEPLDKPCQVGVVRHPALSVGGDGALHLFR
                     SLGAQIVEFLPDSEMPPDLGALWVPHGRALWAADQMVDDPRFRKGLGRIAMQNKPFLV
                     EGESSALLGEHLTVEAPSSSRRGICLVSCQGRFGKTVGYDEERRVELEGLVTGPLMRS
                     GERCRGYWPRHFSLTSPASQRAEWAVMDAKDRGQIATDGWSLKRGVISQMRLELWSCI
                     DGVRRWLSGA"
     gene            11593..12786
                     /locus_tag="PL162_05775"
     CDS             11593..12786
                     /locus_tag="PL162_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HQF91566.1"
                     /translation="MKDFDENFAALGPELLDEAEGMASDIIAWRREFHQFPELAFEEN
                     LTATRVAQVLESTKGIKVHRGFGTPTSVIGVLRGDLSSPALMLRADMDALAMEEETGL
                     PFGSCIPGIMHGCGHDGHMASLLGAAQILSDRRQTLSRPVVFLFQPAEEGKGGARKLT
                     EAKLISSFNIGWALGIHFWPGLPYGQFFTRPGPITALSDKFHIEIQGVGAHAATPHLG
                     IDPIMIAAHVLLGLEHLVSREFDPLDSVVISVGQIESGEAHNIIPERAHLWGTARAFD
                     TAVRDRIQQLMESTVPDICRAFRGTGNVNYWRNYPQSINDPNFSAAVTERASIFFGED
                     MAHILERPLLAGEDFAFYSLEAPSCFMLLGTGGEYGLHHPKYDVPEDLLYLASAWEAF
                     LALTL"
     gene            12833..14332
                     /locus_tag="PL162_05780"
     CDS             12833..14332
                     /locus_tag="PL162_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQF91567.1"
                     /translation="MPFCRLDQIIDDRCNHRRGDKVLWWRGEWWSGGAFLGLVDQCTK
                     ALRQSGFSSGQRLALLLPNSPVFLALVVATWRLGGTVAPLNPLAGPKAVLQALEFARP
                     FAVVVPQETSDEVKKGLLAVTEVISSSLEGPLSTAQGRPHPLETPEIAVLFFTSGTTG
                     TPKSVPLTHANVMDNVAGTIHHLEGLQEGETILNVLPNFHAFGFTVCSILPLAEGLPQ
                     VIMPSFMPAENTLHALIAAEVSVVVGVPTMIALLLGAAARAGVQCPSVKLAVSGGDRF
                     PVHLDGRCQEIFGVGIQEGYGLTETSPVVSINSTARQRRLGTVGSLLPGYEAQVRDVT
                     GKVLPPEEEGVLWLRGPSVASGYFDAPDLTAQRFDDGWFNTGDMVRFDSEGYLSILER
                     VSDLIIVSGFNVFPQDVEVVLRTHPGVRDSAVVGVPHAVSGEIVQAFVVPNHESPVTV
                     RELAAFCRDRMAHYKIPRSWTFVDELPRSSIGKVLKHELRRRCSSSTEK"
     gene            14329..15411
                     /gene="citC"
                     /locus_tag="PL162_05785"
     CDS             14329..15411
                     /gene="citC"
                     /locus_tag="PL162_05785"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR00124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HQF91568.1"
                     /translation="MSPLFPPFEFRILERPGPREKELLGPLLARSGLVFEGTPELSVV
                     AETLEGEVVATGSLEGVVLRMVAVDPRAQEGGLASAVISRLLEEARSRGRHHLFVFTK
                     PDSAERFSELGFVAIARFDPHVVLLEMGEPNVETFRKHLRRIVATDVNGVDSRGVHGA
                     IVMNANPFTLGHRYLVQESRARCDRLFIVVVETDRSTFPFAVRYQLVQEGVADLPGVT
                     VLGGGEYSISPATFPTYFLKHLDDAEQIRLQARLDSVLFSDLFGRELGLSRRFVGTEP
                     LCPVTALYNEALLEILPPRGIEVTVIPRKTDSGGAISASSVRRSWVAEDWEALRRLVP
                     PSTERFLRDRESRPIWERLCRSSGRH"
     gene            complement(15522..16337)
                     /locus_tag="PL162_05790"
     CDS             complement(15522..16337)
                     /locus_tag="PL162_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQF91569.1"
                     /translation="MDGIDGLIFDIDGVLLDISDSYFPMMREVLRRLAEGVSAPLRSG
                     EEFSRDHLRVAKKHPAFNDDYDIPWVFFSFREHALRRGDPADVRAWEEALSLCPPDPS
                     EALVWVQNQWAGRLSRDWVRILCDRLYFGDAYRRVRPESDCTGLGPGFWSEERALIHR
                     PWRSLPLPSAIYTGRSRGELDLVFEKLGWEDFPRGNTVTADDGILKPSPEGLILMAQR
                     LGASFPLYFGDTESDLLAHRSFGRGVFVAVGSVLTEWRPRYPSAEEALRDLFP"
     gene            16454..17836
                     /locus_tag="PL162_05795"
     CDS             16454..17836
                     /locus_tag="PL162_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HQF91570.1"
                     /translation="MIEIPLDFTSSTPLYRQIADHLRAMIQTGALEEGFRLPGTRPFA
                     LSLGISRTTVNEAYGLLASEGFIRQEGRRGAFAQCGDAPSPPSLRQGRRWDLASGTPS
                     TDFLPLRQLGSLWRSVLQEGEKALLMPPVAGLAPLRQALVGHAATRGIPARWQDVLVT
                     SGVQEGLALALGALGRRGVRRLWTETLTYPHLPPLVSFEGQELRLLPENPEDWEDDMG
                     KMTPQDALYLIPSFHNPTGRTLPRSLRERLLQRASARGCWIVEDDAYGELRYGEFAVP
                     ALRALPGAERVLYLGSFSQLLFPGWRLGYALVPQELHGAFFHAKLHLSGATATPSQLL
                     TLAFLRQGLLAPALADVRKRMAHRMGTLHASLAQAFPQIPLEMPQGGVFLWLATPGLS
                     GREAALQARHHGVLVTPGELFSPARRPVEAVRLAVSRTSAPDLLGVGAILARAWKGLP
                     WCTEKTSSPF"
     gene            17806..18351
                     /locus_tag="PL162_05800"
     CDS             17806..18351
                     /locus_tag="PL162_05800"
                     /inference="COORDINATES: protein motif:HMM:NF015742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HQF91571.1"
                     /translation="MYRKDLLALLRGKDGRDTLRRGLLRGPEDCVVQMALNIPGLPKT
                     LPGDRETIERTWLHWKGSFYFSSPRIVAWVKNAAGVALLLMFSGEAPRSLKKAGGRCE
                     EELPWGRLLDIDVYTSRGPLSRALLGERPRRCFLCEGEAKACARAGTHSLISLRDAAV
                     ALMGRAEGGNARGKAPPKSPI"
     gene            18728..19549
                     /locus_tag="PL162_05805"
     CDS             18728..19549
                     /locus_tag="PL162_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HQF91572.1"
                     /translation="MTIDIALRGVSFSFGKRTVLSEVSFSVPSGEFLVLLGPNGGGKS
                     TLLRLILGLLTPSRGTLSVLGMPPAQAVSRLGYVPQDVGRHGDFPVTVRDVVLMGRRG
                     IRPEAASQDASRVEMALEQVDLWAARDLRMGDLSHGQRQRTLIARALCSEPAMLLLDE
                     PTASVDAAIQERLYKLLKKINDTMTIVMVSHDIMAVTSCATAVACVNEQVFYHPRGEI
                     LPEMIQRAYGSCPVELVAHGVPHRVLAPHRAEEETVGRNSHETPRRGPSGGDGGA"
     gene            19546..20346
                     /locus_tag="PL162_05810"
     CDS             19546..20346
                     /locus_tag="PL162_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HQF91573.1"
                     /translation="MIFLDFEFMRHAILAAIFASALCGILGTFVVAHRMVGIAGGIAH
                     AAYGGVGLAFLLHISPTVGALGFAGVAGAVAAWITLRFPHRADTVTGVLWATGMALGV
                     LFVDLSPGYGADLMSYLFGSILTVTREDLVFMGSLLIITVIFLSIFYRDLLAMGYDED
                     FARTRGIPVSFLHTALVILISLVVVVVIRVVGLILVIALLTIPPHIAEGFTRSLKGMM
                     ALATGMALVFSLGGLGISYLCNITAGATIILLASATYILSLILHRFRP"
     gene            20417..21613
                     /gene="truD"
                     /locus_tag="PL162_05815"
     CDS             20417..21613
                     /gene="truD"
                     /locus_tag="PL162_05815"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HQF91574.1"
                     /translation="MIIKEHPEDFRVEERIDLVWDLEPGPFRLYTLEKRSWNTTDAFQ
                     AAARATGIPWGVIRYGGKKDRQACTVQYGTTPAAFDLSFQRHNVRVTPVGFAPTPMGP
                     RHVKGNKFDVVMRRVEKGHGELLGQRWEEMERHGELNYFDDQRFGGVSSSGAFFAEFV
                     VRKDFSGALRHHMTSHHPEASPAIRRRKRSMDRMWGDWRALRSLAVTPEEREMVGCCL
                     ANPGERGILEALGLLSRETWGLYLSSFQSALWNEALALMVRRSSEPLWSLRGKVTSLP
                     FFRHKGTEGAWRSLEIPMPSSELSRSDDEGSRAVGEVLKRHGLKPEDFILTPSFRAHF
                     SSFSRSAVVVPQEGTWGLSDDDRHRGYDKGRVSFFLPAGSYATLILRSLQHSIPGPSS
                     DGPGPG"
BASE COUNT         3970 a         6689 c         6875 g         4503 t
ORIGIN      
        1 ttttcgtcac caaaacaagg catcgcccat ggcccctaaa gggtcgaggc cctcccttgg
       61 ggcctaagcc ttctatcaag cctcctcgcc cggggccctc tggcgagggg acttaaaact
      121 aaagcggggg gcgaggcttc ttgctatccg acgggaggag tgtccgcgat ggggttattt
      181 acccacacag aagcgggaga agatctcgtt cagcagggcg tcgtcccacg tgaagcccag
      241 gagacgatcc agggcctgaa gggcttcttg aaggccctgg gccaagaggt cttctccata
      301 tccggggtcc agggcttcct cggcccgggc caccgcgagc tcggcctggc ggagctcctg
      361 gcattgcctc gccgaggcgt gtagagctcc ctccaaggaa tctttcccca cggccatgtg
      421 ccaaaggaga tctttcaagg ccgtgatgcc tcgctcctgt tgggcggaga tggtgatcac
      481 cggtgcttcg aggcctagga gggagatgag ggcgccttcg tcccagcgtt cgggaaggtc
      541 actcttgttt cgcaccgcca ggaggcgtcc cttctctcgg aggctttttc ccagttccca
      601 atcctccggc agaaggggct cgctggcatc cagtacccaa aggcgtagat cggcttcttc
      661 ggcggcatga agggcccggg ccactccctc ccgctcggcg gggtcctggg cgggtcgaat
      721 ccccgccgta tcgaggaggc gaaagggcac tcctcggtag gatagcacct cttccaccac
      781 gtcccgagtg gtgcccggca gggcggtgac aatggctcgg gattgttgca acagggcgtt
      841 gagcagggag gattttccca cgttagggcg ccccaccagg gccactcgcc acccggtgga
      901 aagcaacgcc cccgatcggg cctgacgttg ggcctcctgg agcctttctc gaattttttt
      961 gaggcgcccg accaagtctt cccgttccca aggagggagg tcttcgtcgg gaaaatccag
     1021 agaagcttgt accgtcgccg ccattcccag aagctctcct cggaggggaa ggagagccca
     1081 tccccagcct ccccccaaag cccgctgggc ggcgtgaagc gcctcgtcgt tccgcgcccg
     1141 gatgagtccc aacacccctt ctgcctgggc caggtccagg cggccattga gaaaggcccg
     1201 ctgggtgaac tcccccggag cggcgggacg acatcccagg tccaggacct ctaagagcag
     1261 gcgctgggct cccagatacc ccccatggca atggagttcc accacttctt ctcccgtgta
     1321 gctccgggga gcggcgaacc acacccacag gatgcgatcc aacacggtcc cttctttgtc
     1381 gagaaaggct ccgtaggcca tgcggcgcgg ggggatttcc cccgccggag tttgaccggc
     1441 gggggaaaat ctcagggaag tcatgtggcg aacgccatcc ccagagaggc ggacgatggc
     1501 gataccccct tctcccgggg ccgtggccag ggcggcgatg ggatcggcca tgggagcttt
     1561 ggttcctact gggccagcag ataggagagc agaagccgcg taccgaagcc cgaagctcct
     1621 ttgggatagt acccgaaggg ctctttgtag aaatccacag cggcgatgtc gatgtgggcc
     1681 caggagatct cggggggcac aaactcttgc aggaacatgg cggcggtgat ggctcccccc
     1741 tggcgtcctc cggagttcag caggtccgcc acagaggact ttatcttctt cttgagcatc
     1801 tcgtcgtcca gcggcaggcg ccagacccgt tccccggtct cgcgactggc gatctgaagg
     1861 gcttccgcca agcgatcatc cgaggagaag agccccgccg tgtagtcgcc gagggccacg
     1921 ccgcacgcgc cggtgagggt ggcgatgtcc accatggcgt ccggcgcgat ctccgaggcc
     1981 agagctagcg tgtccgccag ggtaatccgt ccctcggcgt cggtgttatc gacttcaatg
     2041 gtctttccct tgtaggtacg gaggatgtcg tcaggccggt aggacttgcc gtcgggcata
     2101 ttctccgccg ccccgatgaa ggcgtgaact tcgatgtctg gttttaggcg ggcgataccc
     2161 cgtagggcgc ccaagacggc gcaggctccg cttttatccc ctttcatggt gcgcatgctg
     2221 tcagcgggtt tcagggagag tcccccgctg tcgaaggtga gccccttgcc caccagggcg
     2281 atctttttgc gaggtttccc ttccttgggg acgtaggaca ggtggatcag acggggcgga
     2341 gtggctgacc ccttgcccac ggaccatagg gcctccagcc ccttttgctg gatttgggcc
     2401 tcgtcccaga cctcacaggt caacccttcc tcctgggcca gagcctgggc cctctccgcc
     2461 agagtctgag gattgatcac gttgcccggc tcgttggcga gggcgcgggt gaagatctgg
     2521 ctctcggcca acaccgtccc ccgccccagc ccttccgcgt ggccgtcggc gaggaggatc
     2581 gtttccagag ggtcggtgcc gtttttctcg gcgggggagt ctacggagga ttctttgtcc
     2641 tttttagccg tctggtaggt ccgcgtcggg tcattggcca aaagggcccc ttcggccaag
     2701 gcttgggcca ccaggggctc ttgggcctgg ggggccaaga gcaccaactc tcgtacctcc
     2761 gcccttcggg ctcccctaac tgcttctgcc gccgcccggc gccacgtctc tggtgtggct
     2821 ttgtcccggg ggcccatgcc cacagcgaaa agctgggaga actggctgtc ggggaaaaag
     2881 agtcgcagca tctgatcttt tttcccggaa aattttccca tctcgcactg gcgctggaag
     2941 gatgaagcca tggaacccaa ggaggaggag tggcagagct ccccctcgaa gagcaatacg
     3001 gcgcaggctt cgccgctccg ttgattcgtc caaggcactg atttcacggt aagatgactc
     3061 tcctttctgg ggcggcccta caacatggac cgcatggctt ctccgaggcg cttacaccct
     3121 tcctcggtct gttctgacga ggcgaaggtg tagcacatgc gaaaattgtg agtgcctccg
     3181 cccttaggat agaacgcttc gcccagcaca aaggccacgc acctctcaat ggccttttcg
     3241 aagaggggcc gggcttcgat acggggcgtt tccagccagt agaaaaatcc tccctcgggg
     3301 gtgacccagg ggctgacttc ttccatgggg agatggcgac ggaaggcggc ctccatgtcg
     3361 tcgcgttttt ttcggtagtg ggcgatgatc ttggggagga aactgtccaa atggccccgg
     3421 cgacagtact cgtagaccac ggcctgggcc acgacgctgg cgcacacgtc cactccctgc
     3481 ttaaaaatgg ccatcttgcg caggatttcc gccggtccgg tgcaccaccc cactcgggta
     3541 cccggggcga ggatcttaga gaagcttccc gcgtaaacca ccatgttctc tttatccatc
     3601 gagaacaggg aggggagatc ctctccgtcg aatcggacat acccgtaggg gtcgtcttcc
     3661 agcacgggga tccggtagcg gtgggccact tccaacagcg ccttgcggcg ttccagggac
     3721 atggtgcatc ccagggggtt gtggaagttg acgatggtgt agatgaactt gattttcttt
     3781 ccctggcgtc gggcctcttc gatcttttcc ggaagcaggt cgacgcgcat gccccccgca
     3841 tcgcagggga tgcagatgaa gtcagctccg tggttgtaca tgttgagcat ggttcccagg
     3901 aacgtgggtt cctcgatgat gacgcaatct cctcggttca agagggcgga gctcagcaga
     3961 tccaccacct gggaagatcc ggcagtgatg aggatctcgt tgtcttccac ctggcgcccc
     4021 atacgagggg ccgtccactg gcgcaggaat tctttgaggg ggggataccc ctcggtggtg
     4081 ccgtactgca gcacgtcctt accttgggtg cggagtattt ccgccgacgc ggcaaagttc
     4141 tccaccggga agatctcggg gtcgggcatg cctccggcaa aagaaatcat gcccgggcga
     4201 cggaccatgt gcagcatctc ccggatggga gaggggcgaa tgctcagggc cgcatcgctg
     4261 aacagggtgt tccaaagctc gctcatgaca gaaaacctcc ttgcaaaatg tttgaagctc
     4321 ggagatccgg gtcgacgccc ttcccggggg tgaggcgccc gaagctcctc ttttggatac
     4381 ggtacactcc cgtcccttcc agcataccat actagtccgg tttacaagaa gtttttgtga
     4441 cggtcgaagg acggttcggg ccgagatttc ccaaaaggtt ggccaagacc ttttcccgtt
     4501 ggctcaaggc cgaaaaagct cgcccgagga ctgggcaaaa gagatggttt caggggaagg
     4561 caaaaatatt cccgggaagg cttcgctttt ttgaaagatt tatgctatta tggcgccgaa
     4621 agataggggc ttcccggggg gtgtgccccg tttttggttt tgggccctag ggggccattc
     4681 ggaggagaaa agggtcgacc aaggggaggg atcgacggtg aacacccgga tgcttgcatg
     4741 gccgagctat atggacctgg tcgtgcccag gtctcggcgt ccgtcgcgaa aggcgctcga
     4801 ggatcgtccc tttatgccct ttggcatggc ccttcgaggc cctctgcctc ggacgtcttc
     4861 cgcctcctac gacagcgccc tccttctgtc ccggaagatc tccggggggc gcgtatcggt
     4921 actcgcctgg ctaggggccc ctccgcgcga tttttatggc ggagaggatc gaagggagtc
     4981 attgcccttg gaacctcgcg acccgctcca ggaagaggaa gactcctctt ggtctccgca
     5041 aaggtgggcg gccaagatcg cctacggccg atcgtttcat ttcgactcct ttcccctgac
     5101 ctcccactcc ttctgtgcct gaattcccga agcccctccc gttagggggt cccttgcccc
     5161 tccgccgcgc ccctcgggca ctcggagggg cggcccttcg gtgatccctg ggcgaaatcg
     5221 tcgatcgtca tggcgtttcg ctctaaagcc aaggacgggg aggaaggacc cttgcgtagg
     5281 cggcgccttg agtgtcaaag agcgccaccc atgggcccac ggcggggtag agcgtaagcc
     5341 gaggaatgtc ttccagcgga acccacagga tctcttgggc cttggtgtga tgaggttgaa
     5401 gtcgaggggg ggcctccccg ggggcacacc ggaaaaggat gtgtagcgtg gcgggaagat
     5461 ggtgcatggg ggatgtttct cccgcccata tcagggggcc cacctccacc gtcatcgcca
     5521 gctcttcccg gagctcacgg cgtaacgtgt cttccaggtt ttctccctcc tcctgccctc
     5581 cccccgggag ggaaaagagc gttcctccgg ggtaggcgta gcgcaacatc agtacccggt
     5641 cggagcgggt gatccacgcg gcggggcgaa ttttcatcaa aaaccctcct cggagaagaa
     5701 tcgattcttg ccgtgatatc cactgcctcg tcctccgtcg cctcttcccg tggctcgcgg
     5761 cggaggcccc agatcctgta attttacccc agggtttctt cggaaagctg cacaacggag
     5821 ttttttgccg ggggaaaata cccgggcccg caagagattt ttgcgtcgcc gggaaacccc
     5881 atgaggcaac ccccgtctcg ggagaagatc ccgcttccgc cccgggggcg tcgtgccgcc
     5941 cgtcaatctt gccgtgaaag aacctccgtc catccctaaa ggatccaaca ccgccgggaa
     6001 ataggccacg gaagggcgga gccgaaaacc ccgaggagag ggctccgggc cagcctggca
     6061 atctgctccc ccgggcgttt ttttccttgg ccacaggagt taccatggcg tggtcgtgga
     6121 ttaccggtta tcatagttac ggagagatcg ccgcaaggga gccgtggggg ctgcgtgccc
     6181 ttggtcgtgg tccgatggcg catggcccca gggggcttca tcgaagcacc tatcgccgcc
     6241 gtcgggaatc gcggaagctc ttcccgccga agggcaattt ccggggacga accgacggcg
     6301 atgggcccgc tacccgtcgc agaggtccgc gacctacgtc aatctctttc ccaggattgc
     6361 tccgggaaag gcttaggagg agtggagtga atggccgatc agcaagtatt ttccatcgca
     6421 caggttcgag acctatccga aggaggggtc ttctggggaa agtttgtggc gacttccatc
     6481 acacaaaaac tggacaaaaa ccagaaaccc tactgggacg tcgccgtcat ggatggggat
     6541 ggaaccctgg aagccaaggt gtggagcaat agcgaatggt acgaccgccg ggtcgaggaa
     6601 gaggagcccc ggcgggtcga cccgtcccaa gacgcctttt tttctcggat cagcggatgt
     6661 accgtgggag tccaaggaaa ggtgtcgtcc tacaacggcc gcccccagta cactttttcc
     6721 aaagtgtatc tccttaatca ggaaaaaaat cctccccatg cctatgtccg caagtctcct
     6781 atcccctttg tcgacctgga gcggcgcttt cgggaactcg tggaccgggc cgaagatccc
     6841 gtgaagggct ttttacagca cgtctttcgg ggggagagat gggagaagtt ctccacgtac
     6901 cctgctgcca cctctcacca tcacgcctac gtccacggcc tgctggagca caccgtggcc
     6961 gtggcatcga gcgccttggc catcgccgag gactatatca tccgggattt ttcattggat
     7021 cgatccgtgg tgctgggggg atctctgctc cacgacctgg gaaaggtcga tgcttacggc
     7081 ctggatccca tgcccatgct caccctctcg ggatcagtgc acgatcacat cgccctgggg
     7141 tacgccgcct tttcccgcct ggctgaagag ttctccctgg cacccaccgt ggcgctccac
     7201 ctgggacata tcctgctcag ccaccacggg gcgaaggagt acggatcccc cgtgcttccc
     7261 gccactgccg aggccttggt ggtggcggcg gcggacgaat tggattttcg cctcttcgtc
     7321 tacgaccaca cggtgcgatc catgggagat gtcttgggca tcagcgatta tcaccaggcc
     7381 acagggcggc gattctggaa atggcaagag acgccttccg cgtagaatcc cacgatgaag
     7441 ggaggcggct ggaccggatc ctcaggctcc gttggccaga gatccccctg ggggcccttg
     7501 cccgggccct tcgcacggga gaagtgcggg tggatggcaa aaaaagggcg ggaggagacc
     7561 ccgtgatcgc cggagaaaca atcacggctc cctggcttcc tccgggtctt cctcccgaga
     7621 gagaaggaaa ggccctaccc ttggaaatcc tatgggaggc cccggagctc tgggtcgtga
     7681 ataagcccgc agggctattg acccagcccg gccgggacgc cccggacagc gtggccgcgc
     7741 gggcctgggc ccgggagaaa ggcggggggg cttttcgtcc gactccggta caccgcctgg
     7801 atcgaaacac ttccggggtg ctcttggtgg cccggtcagg ggtaggtctt cgcgccctgc
     7861 aagaggcgtg gcgcctgggg cgagtggaaa aaacctactg ggcggtgctc tggacggggg
     7921 atgaaaaaat ccccgaaggt gcgacggtgg acttccccct gcgcaaagac ccctccacga
     7981 acaccgtgac cgtggccccg gacggagctt tctcccggac gatttttcgt accatgatcc
     8041 gtcggccccc cctggctctg gtagccctgg atctggagac ggggcgccca catcaggctc
     8101 gggtccacgc cgccgcctgg gggtaccccg tgtggggaga tcgtaagtat ggagtgatca
     8161 ctccccttcc cggagcgggc gccccccctc gccctcttct ccacgcccga gaactggggc
     8221 tgccttccct tccggcaccc ctagagggcc tttcggggag gacctttgtg gccccggcgc
     8281 cggaggattt tgtccatttc tttggtcctc tggatggcgc tccccggagg gttgccaaat
     8341 atacccgata ggggtataat tggtctatcg tacgagaagg gggagagggg ccatgggaaa
     8401 gcgttcgttg gagatctccg acaaggctct ccaggtgatc cgcgacaagt ggaacgggga
     8461 ggggtatgtg gtcgttcgcc gggtgggggg agggtgagcg gctcgctacg ccgccctcgt
     8521 ggagggcggc gccccggaag atgcgcaggg gtacgaggct ttggccaagg atggggtaaa
     8581 gatctgggtc cctgccaagc tccggacgga gggaggcgcc gtgcgcgtgg gccatgcgcg
     8641 ctttctgtgg aagatcaccc tctatgtgga aaacgccctt ttagagcgcg cctaaggggc
     8701 gggccttagg gcaaaagggg ccggaagagg gagagccttc cctctttccg gcccctttac
     8761 agcggaggga tatcctctgg aggagcggcc ttttttaggg atgaatgcga tagatcgtcc
     8821 ggggccaggg aatggcctcc cggatgtgct gtaggccgca gatccaggcc accacgcgtt
     8881 cgacgcccat gccaaaaccg ctgtgcacga aggacccgta gcgccgcagg tccagatacc
     8941 agctgtagga ctcctccggt agcccttgtt cacgaatgcg cgtcaggagc cggtccaggt
     9001 cgtcctcgcg ctgggatcct ccgatgatct ccccataccc ttcgggggcc aaaaggtcgt
     9061 cgcagagcac caggtcgctc cgatcggggt gttctttcat ataaaacgcc ttgacctttt
     9121 ttgggtaaca ttctacgaaa agaggcttgt cgtattgttg ggtcaggagc gtttcgtcgt
     9181 cgttgccgaa atcctcgccg tgctggatat cgcttcccag ggagcgaagc ttatccaccg
     9241 cttcgtcgta ggagatgtgg tagaaagggg ccactacctt gcggaggggt tctaaatccc
     9301 tctccaggat cgtcaactgg ggaacgcagt tttccaacac gtcccggacg atggcactca
     9361 ccaggtcttc ctggagcttc atgttgtcgt ggtggtcgta gaacgccacc tctggttcga
     9421 tcatccaaaa ctccgtgagg tggcgccggg tcttggattt ttcggcccgg aaggtggggc
     9481 caaagcaata caccttgcca taggccgccg ccgctgcttc ggcgtagagc tgacccgtct
     9541 gggcgagaaa ggcctttccc gtgtcgaaat agtccagatc gaagagcccc gaagctcctt
     9601 ctcccacggc tccagtcaat atggggctgt ccaccaggag aaaaccccga tccctgagga
     9661 aggaccgggc gctccagatc acctggtccc ggactcgcat cactgcgtgc tgtcctcggc
     9721 ttcgaagcca caggtggcgg tggtccatca ggaaatcgat gccgtggtct tttttcccga
     9781 tggggtattc ctctgaaggg ttttggattg gccgaagacc cgtcaccgtc agctcgaacc
     9841 ctgacggagc ccgctcatcg gcccgcacca cgccagtgac ttcaagggat gcctcgagcc
     9901 acaacgtctt agccaattca aagacctctt cggggaccgc ctgctttacc atcacccctt
     9961 ggatgaaccc cgtgccgtcc cggagctgca aaaagtggat ctttcccgag ctccgcttgt
    10021 tgaaaagcca ccccttgatg gtcacttcgc gattctcaaa atctgccact cgacggatct
    10081 ccacccaagg cgctgccatt ctttttttcc tcctccgtgc gaaccatcgt cgccaggttg
    10141 tgggttatgc tacactatag agcattcttg ctccaaaggg gagagcccat tgcgtcgaca
    10201 tcgcttgttc ttgggagatg aacggaaggt cggatccatc cccttggccg tgctttttgc
    10261 agcggccttg gtcgagaggg ggtatgctct gcgccttttc gttgcgggca tcgacgagac
    10321 gacggtacgg atgctgcagc tcctttgccg tcagcccgtc acggtgttgg atccctatct
    10381 ggcaggggga gacgacgtga tgcgtgccat cttccagtgt gccagcagcg acggcgcgct
    10441 gaacctcatc ataggccctc tgttggccaa ggggtcgaac gaggagaccg tcaccgtaga
    10501 ccgggggtcc ctccgggtgt tgaaggcact ggaaaccccc gtcatgccaa tcctctatgc
    10561 cgatatgtct gcggttttgg cggcccggcg ctgcgagcag gtcttttctc agatcagcga
    10621 gggagccccc cagtctcggc agatgggggt ctgcttcgct tcggtgctga atccccggga
    10681 ataccaacta ttagaaatag agctggggcg ccggataccc ctcatcagca tgggtttttt
    10741 gccgagtcac gttcaccgag atattccggg cgaagacgtt ctttttactg cggattctca
    10801 ctccatgaag atcacttcgc tccgctccgc tgtggtccaa gtgcggcaca tggaggatca
    10861 aatcatatgg cctttggtgg aggccatggg gcagatggcg ccggagtggc cggaagcccc
    10921 tttcccgtgc gaacctctgg acaagccgtg ccaagtgggc gttgtacgcc accccgcctt
    10981 gtcggtggga ggggacgggg ccctccacct cttccggtcc ctgggggccc aaatcgtcga
    11041 atttcttccg gactccgaga tgcccccaga cctgggggcc ctgtgggtgc cgcacggccg
    11101 ggccctttgg gcggcggatc agatggtgga tgatccccgg tttcgcaagg gtctggggag
    11161 gattgccatg caaaacaagc ccttcctggt cgaaggagaa agttctgccc ttctcgggga
    11221 acaccttacc gtggaagccc cctcctcttc ccgaaggggc atttgcctgg tatcttgtca
    11281 gggacgtttc ggaaagacgg tgggctatga tgaggagcgt cgggtggagc tggaggggtt
    11341 ggtgaccggc ccactgatgc ggagcgggga acggtgccgg ggatattggc cccgccattt
    11401 ttccctgact tctcccgcgt cccaacgggc ggagtgggcc gtgatggacg ccaaggatcg
    11461 gggtcagatt gccaccgacg gatggagttt aaagcgaggg gtcatttccc agatgcgtct
    11521 ggagttgtgg agctgtattg acggcgttcg gcgctggctt tccggcgcat agggaaagaa
    11581 aggacatgga agatgaagga cttcgatgag aattttgcgg ccctagggcc ggaattgttg
    11641 gacgaagcgg agggcatggc gtcggacatc atcgcttggc gtcgcgaatt tcaccagttc
    11701 ccggagctgg ccttcgagga aaaccttacg gccacgcgag tggcacaggt cttggagagt
    11761 acaaaaggaa tcaaagtgca ccggggtttt ggtaccccca cgtccgtgat cggagtcctg
    11821 aggggcgatt tgtcaagtcc tgcgctcatg ttgcgtgccg acatggatgc cttggccatg
    11881 gaagaggaga cgggccttcc ctttgggtcc tgtatccctg ggataatgca cggctgtggc
    11941 cacgacggac acatggcatc cctcctgggg gccgctcaaa tcctgtccga tcggcgtcaa
    12001 acgctctctc gccccgtggt ctttctcttt cagcctgcgg aagaggggaa gggaggagct
    12061 cgcaagctga cggaggctaa gctcatatct tcttttaaca ttggttgggc cctggggatt
    12121 cacttttggc ccgggcttcc ctatggccaa ttctttacca ggcccggtcc cattacagcc
    12181 ctctccgaca agtttcacat cgagatccaa ggcgtggggg cccatgcggc cacgccccac
    12241 ctgggcatcg atcccatcat gattgccgcc cacgtactat tggggttaga gcatttggtg
    12301 agtcgagagt tcgacccctt ggacagtgtg gtcatttccg tggggcagat agaaagcggc
    12361 gaagcccata acattatccc cgaacgggct cacctctggg gcaccgcccg ggcctttgac
    12421 acggccgtga gggatcgcat tcaacagctc atggagagca ccgtgccgga tatctgtcgg
    12481 gcctttcgcg gcacaggcaa cgtcaactat tggcgtaact accctcaatc catcaacgac
    12541 ccaaattttt ccgccgcagt gacggaacgg gcgtcgatct tttttggcga ggacatggct
    12601 cacatcctgg agcgccccct cttggcgggg gaggacttcg cattctactc cctggaagcc
    12661 ccttcctgtt tcatgctcct tggcaccgga ggtgagtacg ggctccacca tccgaagtac
    12721 gacgtgcctg aggacctgct ttatttggcg tccgcctggg aggccttttt ggcccttact
    12781 ctgtaatcct gggctccgta tgaccctttg tcacgagagg agttttgtga aaatgccttt
    12841 ttgtcgttta gatcagatca tcgatgaccg ctgtaatcac cggaggggag acaaggtcct
    12901 gtggtggcgc ggggagtggt ggtccggggg agcgttcctc gggctcgtgg atcagtgcac
    12961 gaaagccctg agacaaagcg ggttttcttc ggggcagcga ttggcgctcc ttttgcccaa
    13021 ttctcccgtt tttctcgccc tggtcgtcgc cacctggcgt ttggggggta ccgtcgctcc
    13081 cctgaatcct ctagcgggcc ccaaggccgt ccttcaggct ctggaatttg cccgcccctt
    13141 tgccgtggtc gtgcctcagg agacctctga cgaagtaaaa aaagggctcc tcgcagtaac
    13201 tgaggtgatc tcttcctctc tggagggacc cctctccacg gcgcagggac gccctcaccc
    13261 cctggagacc cccgagatag cggtactgtt ttttacttcc ggcacgacgg gaacgcccaa
    13321 aagcgtgccc ctgactcacg ccaacgtcat ggacaacgtg gcaggcacca tacatcacct
    13381 cgaagggctt caggaggggg agaccatcct caacgtcctg cccaactttc acgccttcgg
    13441 cttcacggta tgcagcatcc ttcccctggc ggaggggtta ccccaggtca tcatgccctc
    13501 cttcatgccg gcagagaaca cgctccatgc cctcatcgca gcggaagtca gcgtggtggt
    13561 aggagtcccc accatgatcg cccttcttct gggagcggcc gcccgggcag gtgtccagtg
    13621 cccttcggtg aaactcgccg tatcgggagg ggatcggttc cccgtccatc tggacggccg
    13681 atgccaggaa attttcggcg tggggattca agagggctat gggctcacgg aaacctcccc
    13741 ggtggtgagc atcaattcca cagcacgcca aaggcgcttg ggcacggtgg gatctttact
    13801 cccggggtac gaggcccagg tgcgcgacgt gacggggaag gtcttgcccc ccgaggagga
    13861 aggagtcctg tggcttcggg ggccttcggt ggcttccggc tacttcgacg ctccggatct
    13921 gacggcgcag cgcttcgatg acggatggtt caacacgggg gacatggtgc gctttgattc
    13981 cgaaggctat ctgtcgattt tggaaagggt cagcgacctg atcatcgtga gcgggtttaa
    14041 cgtcttcccc caagatgtgg aagtggtgct acggacccac ccaggggtgc gagacagcgc
    14101 ggtggtggga gtgcctcacg ccgtgagcgg agagatcgtc caagcttttg tggtgcccaa
    14161 ccacgagtct ccggtcaccg tcagagagct ggccgcgttt tgtcgagacc ggatggccca
    14221 ttataagatt ccccgctcct ggaccttcgt ggacgagctc cctcggtcca gcatcgggaa
    14281 ggtgctcaag cacgagctgc gtcggcgctg ttcctcttcc acggagaaat gagccccttg
    14341 tttcctccct tcgagtttcg gattttggaa cgcccgggac cccgggaaaa ggaactcctg
    14401 gggcctcttt tggcgcggtc gggcctcgtc ttcgagggaa ccccggagct ttccgtggtg
    14461 gcagagacct tggaagggga ggtggtggcc acgggaagcc tcgaaggggt cgtgctccgc
    14521 atggtggcgg tggatccgcg cgcccaggaa ggaggattgg cctccgcagt gatatcccgg
    14581 ctcctggaag aggcgcgctc ccgggggcgc caccacctgt ttgtcttcac gaaacccgat
    14641 tcagcggagc gtttttccga actgggcttc gtggccatcg ctcgtttcga tccccacgtg
    14701 gtgctcctgg aaatgggaga gcccaacgtg gagaccttca ggaaacacct ccgtcggatc
    14761 gtcgcaactg acgtcaatgg ggtggactcg agaggggtcc acggggccat cgtcatgaac
    14821 gccaacccct tcacgctggg acatcgttac ctggtgcagg agtcccgggc aaggtgcgac
    14881 aggcttttta tcgtggtggt agaaaccgac cggtctacct ttccctttgc cgtgaggtat
    14941 cagctcgtcc aggaaggcgt ggcggatctg ccgggagtca ctgttttggg aggaggagag
    15001 tactccatct cccccgccac tttccccacg tactttctaa agcacttgga cgacgccgag
    15061 caaattcgcc tgcaggcgcg cctcgattcc gtgctttttt cggacctctt cggccgggaa
    15121 cttggtcttt cccgtcgctt cgtggggacg gaaccccttt gccccgtcac cgccctctac
    15181 aacgaggccc ttctcgagat cctacctccc cggggcattg aagtgacggt gatcccccgg
    15241 aagacggact ctggtggagc catttccgca tcctccgtgc gccggagttg ggtggcagaa
    15301 gactgggagg ccctgaggag gctggtgcct ccctccacgg agcgatttct gcgagaccgg
    15361 gaatcccgcc ccatctggga acggctctgt cggtcctcgg ggcggcactg acccatcgca
    15421 ctccaagctc ctctccgccg gaagactatg tctcggccgc caggggatct ggagcctcct
    15481 ggcgaggccc tgccgaggag caagaatctc ctgcggcagg gctacggaaa taggtcccga
    15541 agggcctcct ccgcgctcgg atagcgagga cgccactccg tcagtaccga ccccacggcg
    15601 acgaagaccc ctcgtccgaa ggaccgatga gccagaaggt cgctttccgt gtctccgaaa
    15661 tagagcggaa aagacgcccc cagtcgttgg gccatcaaga tgagcccctc tggagagggt
    15721 ttgaggatgc cgtcgtccgc cgtgacggtg tttcctctgg ggaagtcctc ccagcccaat
    15781 ttttcgaaca cgaggtcgag ttctccccga gatcgaccgg tgtaaatggc gctggggagg
    15841 ggaagggatc tccacgggcg gtgaatcaac gccctttctt cgctccaaaa ccccggcccc
    15901 agcccggtgc agtcgctttc ggggcgtacc cggcgatagg cgtctccgaa gtacagacgg
    15961 tcgcagagga tgcgcaccca gtcccgggag agcctccctg cccattggtt ttggacccac
    16021 accagggctt cggagggatc cggcgggcaa aggctcaggg cctcttccca cgcccggacg
    16081 tcggcggggt ccccccggcg cagggcatgt tctcggaagg aaaaaaatac ccaggggatg
    16141 tcgtagtcgt cgttgaaggc ggggtgcttt ttagctacgc ggaggtggtc ccgggagaac
    16201 tcctccccgg agcgcagagg ggcggaaacc ccttcggcta gtcttcggag gacctctcgc
    16261 atcatgggaa aatatgaatc ggaaatatcc aaaagcactc cgtcaatatc aaaaatcaat
    16321 ccatcgatgc catccatgac acccactcct tttgcttgcg gcgatttgga gccgacggga
    16381 gtcgggaccc gccggggatc agcaaggccc cagtatagca gacggggaaa gccttcgaga
    16441 ggagtgggac cctgtgatcg agatccccct ggattttacg agttccacac cgctctaccg
    16501 tcagatagcc gaccatctcc gggccatgat ccagacgggg gccctggagg aaggctttcg
    16561 ccttccagga acccgcccct ttgccctctc cctgggcata agtcgcacca cggtcaacga
    16621 ggcctacggt ctcctcgcca gtgagggctt tatccgtcag gaggggcgcc gaggggcctt
    16681 tgcgcagtgc ggcgacgccc cgtccccccc ttcccttcgc cagggaaggc gatgggactt
    16741 ggcctcgggg accccatcga cggatttcct tcccctgcgg caactgggct ccctgtggcg
    16801 ctccgtcctt caagaggggg aaaaggccct tctcatgccc cccgtggcgg gactcgcccc
    16861 ccttcgtcag gccctggtgg ggcacgccgc caccaggggt atcccggccc gatggcaaga
    16921 cgtgctggtc acctcggggg tgcaagaggg gctcgccttg gccctagggg ccctggggcg
    16981 ccgaggggtg cgacgccttt ggaccgaaac cctgacctac ccccacttgc cccccttggt
    17041 ttcttttgag gggcaagagt tgcgcctctt gccggaaaac cccgaagact gggaagatga
    17101 catggggaaa atgactcccc aagacgccct ctatctcatc cccagctttc acaaccccac
    17161 cggccgcacc ttgccccggt cccttaggga acgccttctt caacgggcga gtgctcgggg
    17221 gtgttggatc gtggaggatg acgcctacgg cgagctgaga tacggggagt ttgccgttcc
    17281 agcccttcga gcccttcccg gggccgagcg cgtcctatat ctgggatcgt tcagccaact
    17341 tctttttccc ggatggcgcc tggggtacgc cctggttcct caggagctgc atggcgcctt
    17401 tttccacgcg aagttgcacc tttccggagc caccgccact ccgtcacagc ttttgaccct
    17461 ggcctttctg cgccaggggc tgttggcccc tgcgctggcg gacgtacgca agaggatggc
    17521 ccatcggatg ggcacccttc acgcctcact ggcccaggcc tttccccaga ttcccttgga
    17581 gatgccccag ggaggagtgt ttctctggct cgccactccg ggcctctccg ggcgcgaagc
    17641 ggcgctccag gctcgtcacc acggcgtttt ggttactccg ggagagcttt tttctcccgc
    17701 cagacgaccg gtggaggcgg tgcgactggc cgtaagccgc acttccgctc cggacctctt
    17761 gggagtggga gctatcctgg cccgggcgtg gaaaggactt ccctggtgta ccgaaaagac
    17821 ctcctcgccc ttttgagggg caaggacggg cgagacacct tgcgaagagg ccttctgcgg
    17881 ggccccgaag attgtgtcgt gcaaatggcc cttaacatcc ccggcctccc taagaccttg
    17941 ccgggggatc gggaaaccat cgaacgaact tggctccact ggaaagggtc gttttacttc
    18001 tcctctcccc ggatcgtcgc ttgggtgaaa aacgccgccg gagtggccct attattgatg
    18061 ttttccggag aagccccccg gagccttaaa aaggccggag gccgctgcga ggaggaactc
    18121 ccctgggggc gcctactgga catcgatgtg tatacttcta gggggcctct gtcccgcgcc
    18181 ctcctggggg aaaggccccg acgatgcttc ctctgcgaag gagaggcaaa ggcctgtgcc
    18241 cgggccggaa cccattcgct gatctccctg agggacgccg cagtggccct gatgggccgc
    18301 gccgaagggg gaaacgcccg aggcaaggct ccccctaagt caccgatcta gaggcatggt
    18361 tagatgggga cgacctggta ctggagccat tcaagtccac cccagggagc gccccttgaa
    18421 gaagggcttg ccaaaccccg gcgcggggac taccatgctt cccgggttta tcaccctgag
    18481 gggtccccgg aaggcttttg cctctcggga gaaccttcgg atcttgcatc gagacgggac
    18541 cggatcccgc acgaaatacc cctttctaaa gactggaggc gcttcgtggc ttgtcatctc
    18601 cccgggatga cggggtcctg ggcctcgttc ctgcgcgccc cgggcagagg ggcttttctc
    18661 gaatcccccc ctcaccacgg tcctaggtcc gttgcgtccc ccgggagtcg tcctaaggag
    18721 agtgtggatg accatcgata tcgcccttcg gggagtttct ttttcttttg gcaagagaac
    18781 ggtcctttcg gaggtctcct tttcggtgcc ctctggggag tttttggtgc ttctcgggcc
    18841 caacggggga ggaaagagca cgctgttgcg gcttatcctg ggacttttga ctccatcgcg
    18901 aggtaccctc agcgtgttag ggatgccccc cgctcaggcc gtatcccgct tggggtacgt
    18961 cccccaagac gtgggaagac acggagattt tcccgtcacc gtacgagatg tggtcctcat
    19021 ggggcgccgg gggatccgcc ctgaggcggc atcccaggat gcgtcgcggg tggagatggc
    19081 gctggaacag gtggacctgt gggccgcaag ggacctgcgc atgggagacc tttcccacgg
    19141 tcaacgccag cgtaccctca tcgctcgagc cctctgctcc gagcccgcca tgctcctttt
    19201 ggacgaaccc acagccagcg tggacgccgc gatccaggag aggctctata aattattgaa
    19261 aaaaattaac gacaccatga ccatcgttat ggtgagccac gacatcatgg cggtgaccag
    19321 ctgcgccacg gcggtggcct gtgtgaacga acaggttttt tatcacccca ggggagagat
    19381 cctacccgaa atgatccagc gggcctacgg aagttgtccc gtggagctgg tggcccatgg
    19441 cgtaccccat cgagtcctgg cgccccacag ggccgaagaa gaaacggtag gcagaaactc
    19501 ccatgagacg cctcggaggg gcccttcggg aggggacgga ggggcatgat ttttctagat
    19561 tttgaattta tgcgccacgc gatcctggcc gccatcttcg ccagcgccct ctgcggcatc
    19621 ctggggacct ttgtggtggc ccaccgcatg gtgggtatcg ccggaggcat cgcccacgcg
    19681 gcctacgggg gagtggggct cgcctttttg ctccacattt cccccacggt gggggccctg
    19741 ggctttgcgg gcgtcgccgg ggccgtggcg gcgtggatca ccttgcgatt tccccatcga
    19801 gccgatacgg tgaccggcgt cctatgggcc acaggcatgg ccctgggggt ccttttcgtc
    19861 gacctctccc cgggctatgg agccgatctc atgagctacc tttttggcag catcctcacg
    19921 gtaacgcggg aagatttggt cttcatgggg agcctcttga tcatcacggt cattttttta
    19981 tcgatcttct accgtgacct tctggccatg gggtacgacg aggatttcgc ccgtacccgg
    20041 ggtatccccg tatcatttct tcatacggcc ctggtgatcc ttatctccct ggtggtagtg
    20101 gtggtcatcc gagtggtggg actcatcctg gtgatcgccc ttttgaccat ccctccccac
    20161 atcgccgagg gctttacccg atccctaaaa gggatgatgg ccctggccac agggatggct
    20221 ctggtctttt ccctgggggg attggggata tcctatctct gcaacatcac ggcgggagcc
    20281 accatcatcc tgctggcttc cgccacctat atactctccc tgatcctgca tcgcttccgc
    20341 ccctgatttg cccggtcccc ggcgggaaaa cgggaaccgg agacagggga aagccccctg
    20401 aaggaggagc ccctttatga tcatcaaaga acatcccgaa gacttccggg tggaggagcg
    20461 catcgaccta gtttgggatt tggaaccggg cccctttcgc ctctacaccc tggaaaagcg
    20521 atcctggaac acgacggacg ccttccaggc cgccgcccgg gccacgggga tcccctgggg
    20581 ggtaattcgc tacgggggaa agaaggaccg acaggcttgc accgtccaat acggcaccac
    20641 tccggccgcc tttgacctct cttttcaaag acataacgtg agggtgaccc cggtggggtt
    20701 cgctcccacg cccatggggc cccgccacgt caaggggaat aaattcgatg tggtgatgag
    20761 aagggttgaa aagggccacg gggagcttct gggccagcgc tgggaagaga tggagcgtca
    20821 cggtgagtta aattattttg atgaccagcg ttttggggga gtgagctcct ccggcgcctt
    20881 cttcgcggag ttcgtcgtca gaaaagactt ctccggcgcg ttgcgccacc acatgacatc
    20941 acatcacccc gaggcctcgc cagcgatacg tcgtcggaag agatccatgg accgaatgtg
    21001 gggagactgg agggccctgc gctccctggc ggtgacgccg gaagagcggg agatggtggg
    21061 gtgttgcctg gcaaatccgg gggaacgggg tattttggaa gccttagggc tcctttcccg
    21121 agaaacatgg gggctctatc tgtcgtcctt ccagagcgcc ctgtggaacg aagctttagc
    21181 cttgatggtg cggcggagtt cggagcccct atggtctctc cgaggcaagg tcacatcgct
    21241 ccccttcttc cgtcacaagg gcaccgaggg ggcgtggagg tcgctggaga ttcccatgcc
    21301 gtcctccgag ctttcgcgaa gtgacgacga ggggtcccgg gcggtggggg aagtgctaaa
    21361 gaggcacggc cttaaacccg aagattttat cctcaccccg tcatttcggg cacacttttc
    21421 ctctttttct cgttccgccg tggtggtgcc ccaggagggg acatggggcc tttccgacga
    21481 cgatcgtcat cggggctacg acaaggggag ggtatctttc ttccttcctg cagggagtta
    21541 cgccaccctt atccttcggt cgcttcagca ctccatcccg ggaccttcct ccgacggtcc
    21601 cgggccgggg taggtgtgtg gcggcccacc cttcctaaag aggtccgacc ggggagactt
    21661 gccgcgggga atatcgggga aaaattcctc tgcatggccc caaaccccac ggagaccggg
    21721 accgggaggg gcggtcgttt taccccgaag gcgacgggat cctaagatct tcgacgggaa
    21781 aaaggttctt gatgacgtag gaagtcgtca cagtggatgc ttttgtgagt catggccccc
    21841 ggggggttgc aaaaaacttt ccaccgtgct acactcactc cgccgagagg tgcggccgag
    21901 gccattcccg aggacaccgc agttatcgcc tcccctctgc gtcgcttaac tcgttgcagg
    21961 gcaacaagtt gcagggcgga aatagctcag ttggtagagc atcagcttcc caagctgagg
    22021 gtcgcgggtt cgagtcc
//
LOCUS       DAOOER010000033        21294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_2895_length_21294_cov_9.445077, whole genome shotgun sequence.
ACCESSION   DAOOER010000033 DAOOER010000000
VERSION     DAOOER010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 21294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21294
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2895_length_21294_cov_9.445077"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            175..1044
                     /locus_tag="PL162_05820"
     CDS             175..1044
                     /locus_tag="PL162_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQF91575.1"
                     /translation="MIRIENVTKRFGAREAVRGVSLHLPRGTLCGLVGPDGAGKTTLL
                     RMVAGVLAPTAGSITVGGARGYMPQRFSLYEDCTVEENMTLFGTLQGLRGKDLPQRSQ
                     ELLRRYDLLPFRDRLAGRLSGGMKQKLALSAALLSEPEVLILDEPGTGVDPLSRREIW
                     RALHGMHREGRTIVVATPSMDEAQLCSFLVFLSRGRVRERGTPGEICAAYPWTVLRVV
                     GPERRDLREVLAACPCEECHAFGAAWHLAVRDEESARRALEEALARFGIEGYEISRVP
                     PSLEDVFVFREEE"
     gene            1048..1956
                     /locus_tag="PL162_05825"
     CDS             1048..1956
                     /locus_tag="PL162_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQF91576.1"
                     /translation="MNAVDVRGLTKRYGNFSAVEDLSLGVPRGQVYGFLGPNGSGKST
                     TIRMLCGLLAPSGGEGTVLGVDFRKNPKELRQRIGYMSQKFSLYADLTVLENLRFYAG
                     LYGLGGGALRRRVDTMVALADLEGQEKSLTGTLSAGVRQRLALGCALMHHPELLFLDE
                     PTSGVDLRSRRRFWDLIYDLAAEGTTVLVTTHFMDEAEHCDHIGFIFRGRLVATDTPE
                     GLKKRFPGTIYALETPSPPALLENLEEEKIPGVRDLYVFGPRLHAVVEGALPEALGRW
                     SPREIPPSLEDVFVALVQDNERKGER"
     gene            1956..3068
                     /locus_tag="PL162_05830"
     CDS             1956..3068
                     /locus_tag="PL162_05830"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQF91577.1"
                     /translation="MGRRLWALLVKEFIQMRRDRVTFGIMVALPVAQLIIFGFAINTE
                     VKHLATLVFDQSRSAESREMRDAFVATGYFDVLREVESFSEIEKGLQSGAASVGIVFP
                     PDFAPRLQRRQGAPLQVVVDASDSMSAGSAISAAQMLGMVKGQELMARWGMTPMTVYD
                     LRLRAWYNPDFVSPFYMVPGTLGMILTMTMVMITAAALVREREQGTMEQLVVTPVRPL
                     EIMLGKVLPYVIVGYIQLSLGLAVGVVFFDVPVRGSLTLLYLLTLPFIAANLFLGILI
                     STVARTQMQATQMSFFTFLPSILLSGFVFPREAMPEIFFQFSRILPLTYFLRIARGIM
                     LKGAGWDALGGDARALGVLIAIFATVSLMVFRRRAE"
     gene            3152..3577
                     /locus_tag="PL162_05835"
     CDS             3152..3577
                     /locus_tag="PL162_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91578.1"
                     /translation="MAPLETGKKKTRKMILSVVVGADVNEDRVVSAEVFSSSQEARRY
                     LRFVRSSHPRVKYMVLRSELMVSEGEPLEVGRRLWVVMLCDGPTPRQLSIFFHELHAA
                     HHEIPLRQKHVAKFTGKIIVSAWPCELDGAVLPPGPEEG"
     gene            3776..5137
                     /gene="lat"
                     /locus_tag="PL162_05840"
     CDS             3776..5137
                     /gene="lat"
                     /locus_tag="PL162_05840"
                     /EC_number="2.6.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0045484 - L-lysine 6-transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HQF91579.1"
                     /translation="MSGCKWHVAAEEVFPAIERWMLRDGFDIVIDMEKSQGSTIVDAC
                     TGDEWLDFYTFFASSPFGMNHPKLASDEFKEKIFRSAINKVANSDIYTREMGEFVKTF
                     GEVAVPPPFKHLFFVDYGSLAVENALKVAMDWKVRRLLASGKISQGEAYNGSRGTKIL
                     HFNEAFHGRSGYTLSLTNTNDPNKHQMFAKFTDWPRVLNPKITWPLERHLGEVEWLER
                     TAINQIERALANNPNDVCAIIIETIQGEGGDNHFRSEFLQALRHICDAHDLLLIFDEV
                     QCGMGITGKMWAFEHHGVTPDIFAFGKKAQVCGIAVGPRIDQVPDNCFAVSSRINSTW
                     GGTVADMVRATRYLEIYRDEKVLDHVATVTGPRLMKGLQELEAEFPHLISNVRGKGLM
                     CAYDFPCEKRRNEFKVGCWEKKMLILPCGNVSMRYRPALNVPVEAIDRSMEISREVLK
                     KMG"
     gene            5317..6537
                     /locus_tag="PL162_05845"
     CDS             5317..6537
                     /locus_tag="PL162_05845"
                     /inference="COORDINATES: protein motif:HMM:NF022727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3084 domain-containing protein"
                     /protein_id="HQF91580.1"
                     /translation="MGILGEMNWSLIFSLIALSAVVAYVGDRLGMRIGKRRVSVLGLR
                     PKHTSSVVTAFTGVLITVVTLGVLSATSDTVRTALFSMKMVQRQIAELTSQLQDSREE
                     YADLEKRLFDSQGELIEKQRRTGEAERALAQSSLALKAMEEQVQSAQERASRAEEIRR
                     ELEQDRDKLQTERAALQRDLEGLQNQRASLEREIEDTRQRLMKVREGWIVARAGEVVA
                     QGPLEAGADGAALRRALEHMKARARLFLHRNMPAGEPTPEVVVPERAESQVESYLEAH
                     EGQRVALRLVVSANALRGEAVQVRLEPLESKKIYEAGAVLATKTFPASMDKNDAELAL
                     SIFLRDVGQEAAARGVLRNPLTGDVGEIDAGDLAEGVRRLTASAESVEMAVVTLGEIF
                     TEGPLRVKLRVPES"
     gene            6576..6803
                     /locus_tag="PL162_05850"
     CDS             6576..6803
                     /locus_tag="PL162_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HQF91581.1"
                     /translation="MLIVSCQNCGEMKILAGAPDADGLARAMWLCPCCGAGQVVQLPV
                     AVDARGGDLRRICGGLSVGARGEYVSAEACH"
     gene            6816..7238
                     /locus_tag="PL162_05855"
     CDS             6816..7238
                     /locus_tag="PL162_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQF91582.1"
                     /translation="MPLGEYRFYDSKRAVDAEGLHALYRFTQWGRSRALEDISLMLEN
                     SSLCFCAHFEGRVVAFCRVLTDFVYRASLWDILVHPDHQGKGLGTALLDYALHHPALR
                     SVPVVMTYTRDLAPFLVRLGFHPEEGALMLLRRPIEYS"
     gene            7428..8639
                     /gene="metK"
                     /locus_tag="PL162_05860"
     CDS             7428..8639
                     /gene="metK"
                     /locus_tag="PL162_05860"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HQF91583.1"
                     /translation="MAGCPTNHRYLISSESVTEGHPDKLADQISDGVLDAIFAEDPMG
                     RVACETFVTTGLVMVGGEITTSCYVDIPRLARSIVKEIGYTRAKYGFDGDTCAVLTAI
                     DEQSPDIAQGVDKALEVRENDMDDDAIDKIGAGDQGMMIGFACDETPEFLPMPIALAH
                     RLARRLSVVRKECILPYLRPDGKTQVTLEYVDGKAVHADTIVVSSQHHPSVDHAQIAA
                     DLTEHVIRPVVPAEIYDHRTRVLINPTGRFVLGGPMADTGLTGRKIIVDTYGGVVPHG
                     GGAFSGKDPTKVDRSAAYMARYAAKNVVASGLAQRCQIQVAYAIGVAHPVSIMVETAG
                     TGVVPDERLTEMVREHFDFRPAAIIRDLDLRRPQYRRIAAYGHMGRIDLDPMPAWERT
                     DKAEALRAAAQ"
     gene            8676..9389
                     /locus_tag="PL162_05865"
     CDS             8676..9389
                     /locus_tag="PL162_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HQF91584.1"
                     /translation="MPLPSLGPLCRKTLAVFRHFLWPAVCPCCGALGELACPSCLDAL
                     ATHLGPRCSACGGAYPCFPHSAPPLWGPVPYGGPARELVHRLKFRRERALGALMGSSL
                     GRCLPPPEGAALLVPVPLHRGSSRGYNQSEEIARGLGREWSLPVVDALQWTQDWDPQS
                     RRVKASRRDIPLEALRWEGPPRGGVVLVDDVCTTGTTLDRCSRAARRGRSSVVAWVAW
                     SLSEGGIAHATRGLPGEEI"
     gene            9355..9756
                     /locus_tag="PL162_05870"
     CDS             9355..9756
                     /locus_tag="PL162_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91585.1"
                     /translation="MQRGDYPVRKFDLCACVVCGKAFACRPENRTLCPDCRQEVDALY
                     EDVKDFLRANPRMAPTAEQLADLLGVEEKKIQALMADGRIAEVEDPRRRDRRCQSCGE
                     RITGGNLCPQCMAKVHQNTLRASRKDLYSRR"
     gene            9872..10156
                     /gene="flgM"
                     /locus_tag="PL162_05875"
     CDS             9872..10156
                     /gene="flgM"
                     /locus_tag="PL162_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HQF91586.1"
                     /translation="MIERIHGIAGTLPAQKVKNAYRPFSSGEGPGDGVQVSEFSKLLS
                     RATAEAERLPDVRSEKVEQLRQQVESGSYALKLGVVAANLVAAGILKDEV"
     gene            10161..10607
                     /gene="flgN"
                     /locus_tag="PL162_05880"
     CDS             10161..10607
                     /gene="flgN"
                     /locus_tag="PL162_05880"
                     /inference="COORDINATES: protein motif:HMM:NF016985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FlgN"
                     /protein_id="HQF91587.1"
                     /translation="MEAALVDAMGRQTAVLLQMWEIVVQQREALKSGRLPVLQDLMRS
                     LQGISAQARTEEASRDREARRLAAALGCDPCVSALAEALGDGGGGRLRGAAEALTQAV
                     KQLRAETQLLSRLVEESLALQQLLLAEYRRLEGSMTGTLPNMDFRG"
     gene            10678..13305
                     /locus_tag="PL162_05885"
     CDS             10678..13305
                     /locus_tag="PL162_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod C-terminal
                     domain-containing protein"
                     /protein_id="HQF91588.1"
                     /translation="MLNSFFPLEMGRRALTYFRQGMETAGHNISNAAVEGYSRQRVDV
                     SATDPFTDPGLNRPGLPGQIGTGVGVDAVRRLRDLFLDTQYNQEGTTKGYWDKIADVL
                     HTIELFVHEPNADLESPTEVAGAQGQGFKAAMDSFWSAVQEVEKMPDSAANRENLLQK
                     AQTMTTLMKEMVANSEEFRASLNKELKLRVEEVNTLLDEVGALNRTIAEVENVGWNPN
                     DLYDQRDLKVTRLYQLINCSLKCPCDPDDGDFRIYLDGRELVQGQEVRHLKLVPMEGN
                     KSFFDIQVEDNEFDPVRDGEGVEVIIEQRAAEGIHTMEVARVASETRWKIGKGNPSTD
                     TYVGRIEPPRGDENAALGISGSFRLSVGTSGVESRSRTLPGGVLLTAPPVGNQTKDYA
                     FQVSYKDVSRVIQVKWDESGALATHHWTITDGTITHHAAAGSDLKLSDFASTGGTPGF
                     FDKTSSFPALTVESTGGVLRLKSAEDHLLSLEDIRGDLLSGKLQMANPSSIPVTIAVT
                     EEDSLATIRNKINGAFAATENAPRRPEEWLHAAVEQDGEGHTYLTLESHAVGEAYRIN
                     VLGDANGSFYMAKRLGLVDADVNPPHIPGGAHIMERAEDALFAFDGTKYLSATNTFQE
                     ARKLSSEDGYRAATLSTVSEGLRFRIDPTKWRRSEPPTTIDIQHHVKGGELRGYMEAR
                     DDRILSYLDYFDTLVYRFVTETNAVHYGGHGAGVNSLATGNALFDPLTGPSGAARTFG
                     VSDVIMKNPSLLAAAIDDGRGRSRGEGDGANALRMAQLKHAKIMASGTSTFNEHYEAF
                     VGRLGAETQRADSMSRNQKHLVEQIDLQRQSVMGVNIDEEMMDIVRFQQAFNAMARFI
                     TTTDEMLDRIINGLGTVGR"
     gene            13318..17100
                     /gene="flgL"
                     /locus_tag="PL162_05890"
     CDS             13318..17100
                     /gene="flgL"
                     /locus_tag="PL162_05890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HQF91589.1"
                     /translation="MPRITNPMMTNIVLGDMHNNLARMLKMQHQIATNKKYDRPSDNP
                     VDVVRDLSLGTNIYENSQFIRNMDDGMTWLKNSEGAFNHVTSVAQRIRELAIYAGNGA
                     LEGVDMEAIATEMAELQEELRNSANFSVAGRYLLGGLDVTESPFVRDENGSVVYRGNE
                     YHAEFEMGRGVTGQVSFHGREIFPVNERQYAIRGTEVPLDFSWQGRPEILQFQVGDRM
                     AKVHLSELWGDDNSNGKDEPTDFNQFRDPGELRGKTLKEIADHIQASLAMGDVGRLVS
                     VEAKSDPTRGVQWLEIRGHTGEPVRVTSWPETDFAKQPDAAFSNEMGLGGAWTAGSAG
                     RINIAFDNGEVGSVDVALGNDLDAVAKSINENASGLWAQVKENGTRQTLVLYAKDRER
                     TFRVSGTGGAEELFATYDYAHPTTPGVYPVTATEKNSRRVDAIPGATSEALDPPAVGV
                     LNDTLRFRWKDGYTKTLTLAGGEDIDAVAAGINGLYDPTADPTDAGHALWAEVRTTET
                     GARQLFVYAIDGSMGFEVSATGASTTQYFPATRESTALFRTQEPDHSHLDFSSLMRLE
                     TSVKSVEVPAASGVLRPAGGGELHFHLESGGRQTELIIADDGVPLTIDDLAARLRGVA
                     GSWLDVTVESDAPEGNAYDLLGRADANAEPATKRLVLRSKDGQGVAIFDHNPVPGTGT
                     APVPDNRFAAQWGLSTATYGAGGAAFPAGTTLDPYMPALMEVSVGDKAYQVKLYASGE
                     ESILTGGVVDAAKLAAQIQKQVGSDILGYHVANPTNAASAFALYAKTGEPLRIVDLPF
                     ADPNVEDFSGGLARSAGMATGVLGGGVLDTATAGAAGLIRIRTGAKTVEISVRATDTL
                     RDLAHRIKKEAGEWVDVAYYDPRVPDPNHPATGDIVQLSLASKDGSPLSVFDVSGRGA
                     ATFRIDTALRSTADVSGWAAAAGDRLTLKVDGYEHTIDLWDANAIPPEPLAAGKNLDG
                     LVSAINARFQGKDVVAEVVDAGVGDRRLVLSSPRGYVVEVVEARTQLTNSLHVPPAAL
                     RSTANVSGWMAAAGESLDLTVDGTLHTITLTGATDLNGLVDAINAEFQGTGAVAEIVD
                     AGGGVRHLVLSSPQGYTMTANEAGLVAPLHLAASVAPPQRRGGQGPMNQVTTVRTAAN
                     QKATDFFGVMEDLVSAVREEDRQGISGSLLKRIDTFMDNLLKRRTQAGAMLLRYETSQ
                     RRLTDNNTNLTDLRSKVSEPDLAEALTKFNMAQAVYQASLSTIARIVQPTLVDFLK"
     gene            17156..17644
                     /locus_tag="PL162_05895"
     CDS             17156..17644
                     /locus_tag="PL162_05895"
                     /inference="COORDINATES: protein motif:HMM:NF014660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HQF91590.1"
                     /translation="MRKIDTVRFGTLEVEKESLLHFPQGIPGFFHLKEWFLVGEDDNP
                     IKWLQSVGNGAVALPVALPRSVCPEYKARIAEEDLEDLGDEGEICSFVVVNIPSGAPW
                     KSTVNLRAPIVINHRTRRGRQVISLNEEYSMRAPLFSEEEREVLVAQVAPGEDLGVAE
                     EK"
     gene            17646..17894
                     /gene="csrA"
                     /locus_tag="PL162_05900"
     CDS             17646..17894
                     /gene="csrA"
                     /locus_tag="PL162_05900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HQF91591.1"
                     /translation="MLVLTRKVGESLLIGDEVEVTLVEVRGDSVKLGIQAPRSVGVWR
                     KELVQEVARENLQATRGDVPTQVLRWLGRSPDRPSEKK"
     gene            complement(18163..18786)
                     /locus_tag="PL162_05905"
     CDS             complement(18163..18786)
                     /locus_tag="PL162_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91592.1"
                     /translation="MGQDNRARGLRREDARNTPYHYENYNIFYRLFTLLAKDALLPEE
                     RFRFLGDRAFPYDQAATALCVLDSARISSGHFFSSAGCDVCQEACRFSGWCRGNIIGR
                     LKIKLKGQGRGRERVAGSAHFPAGICLFLTKKPMGVHILTISREHQSPLHRPMRRNEN
                     PEDDKKIKKPEEGQLEAGAPGSRAQGTPISAPPAYPQGAKTQKMMHK"
     gene            18869..20347
                     /locus_tag="PL162_05910"
     CDS             18869..20347
                     /locus_tag="PL162_05910"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HQF91593.1"
                     /translation="MSMLFVELGDKERFAVRIELLPDPDKGHYASPEEALSWGRFDLW
                     VQGRNLCAPHGVTWYLLPLFEWFVHNWNALFHESRLPILSDDETWPWVRRDDDARSFP
                     YLSDEEADRREALWYEWSLRHAWRSAAEGGLFPDLLLLREGDFVRFSWGPPPPVGTPP
                     GPVFEHLRGDVLVPLNAVCTPLFDALRDMSDFMIQQGEAWALEDLSRLEALRKNFQEL
                     RTVAPEERLIWLSGIARTLEGARQKVEMLKDRLGDSFSAFALDGISQNELVLEGSSLA
                     SMMYGSTSPEIGEKDVLLLARSVMTLPPSDVAPPRIPATDFSRPFLEGYGIADRLHDH
                     LGTDMTCPSPIDMEDVLRRLGVYTASLDLDDECVMGVAVLRSGCAPSILVNEKHEKNR
                     TPQGRRFTLAHELCHLLIDREHGRPLAVASGPWAPRAVEKRANAFAAMFLMPKPMLDS
                     MAVECSENALVDAVAVKLKTGKLATKWHLRNLGFLPEDVESV"
     gene            20958..21176
                     /locus_tag="PL162_05915"
     CDS             20958..21176
                     /locus_tag="PL162_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91594.1"
                     /translation="MVRPLALLRRDLLDDDGHPHHGAVVPSFEQAAVPVHPQGEVPPA
                     PAPRDLHVEQHPRTICGTGGCVAIAMQH"
BASE COUNT         3987 a         6699 c         6845 g         3763 t
ORIGIN      
        1 cccccaccgc acgttttccg gccaggtgac ggagatcggc caggaggcgg agtttgcccc
       61 ccggatgtcg ttgaaccaga aggagcggac gagcctggtc tttcgggtga aggtggcggt
      121 ggaaaacggt gagggcgtgc tcaagcccgg catgccggcg gacgtgcgcc tgccatgatc
      181 cgcatcgaaa acgtcacaaa acgcttcgga gcccgggaag ccgtgcgggg cgtgtcgctc
      241 catctccccc ggggcaccct ctgcggcctg gtggggcccg acggggcggg gaaaaccacg
      301 ctgctccgga tggtggcggg ggttctggct cccacggcgg gaagcatcac ggtggggggg
      361 gcacggggat acatgcccca gcgcttcagc ctctacgaag attgcaccgt ggaggagaac
      421 atgaccctct tcggcacgct gcagggcctt cgggggaagg acctccccca gcggagccag
      481 gagcttctgc ggcgctatga cctgctcccc ttccgggatc gcctggcggg caggctttcg
      541 gggggcatga aacaaaaact tgccctctct gcggccctgc tttccgagcc ggaagtgctg
      601 atcctggacg aacccggcac gggggtggat cccctgtcga ggcgggaaat ctggcgagca
      661 cttcacggta tgcaccggga ggggcggacc atcgtggtgg ccaccccctc catggacgag
      721 gcccaactct gctccttcct cgtctttctc tcccgggggc gggtgcggga gcggggcacc
      781 cctggagaga tctgtgccgc ctacccctgg acggtgctac gggtcgtcgg gccggagcgc
      841 cgggatcttc gggaggtgct ggcggcgtgt ccctgtgaag agtgccatgc cttcggagcg
      901 gcgtggcatc tggcggtgcg ggacgaggag tcggcccggc gggcgttgga ggaggcgctg
      961 gcgaggtttg gcatcgaagg ttacgaaatc tcccgggtgc ccccttcgct ggaagacgtc
     1021 tttgtctttc gcgaggagga gtgagcgatg aacgccgtgg acgtccgggg gctcaccaaa
     1081 cgctacggga acttctccgc cgtggaagac ctctcgctgg gcgttccccg ggggcaggtg
     1141 tacggcttcc tcggccccaa cgggtcggga aagagcacca ccattcgcat gctctgtggc
     1201 ctgctcgccc cctccggagg ggagggcacg gtcctcggtg tggacttccg aaaaaatccc
     1261 aaagaactcc ggcagcgcat cggatacatg tcccagaagt tcagcctcta tgctgacctc
     1321 accgtgctgg aaaacctccg cttttacgcg gggctctacg gcctgggagg gggggcgttg
     1381 cgccgtcggg tggataccat ggtggcgctc gcggatttgg agggacagga aaagagcctc
     1441 accggcaccc tctccgccgg agtgcggcag agactggcgc tggggtgtgc tctcatgcat
     1501 cacccggagc tcctcttcct cgacgaaccc accagtggcg tggacctgcg ctctcgccgc
     1561 cgcttctggg atctgatcta cgacctggcg gcggaaggga ccaccgtgct ggtcaccacc
     1621 cacttcatgg acgaggcgga gcactgtgac cacatcggtt tcatatttcg tggccgactg
     1681 gtggccacgg atacccccga agggctcaaa aaaagatttc ccggcaccat ttatgccctg
     1741 gagaccccct ctccccccgc cctcctggag aatctggagg aagaaaaaat ccccggcgtc
     1801 cgggatctgt acgtttttgg cccccggctt cacgcggtgg tggagggggc gttgcccgag
     1861 gccctgggcc gatggagtcc ccgggagatc cccccctcgc tggaggatgt cttcgtggcg
     1921 cttgtccagg acaatgagcg gaagggagag cggtgatggg acggcgtctc tgggcgctcc
     1981 tggtcaagga attcatccag atgcggcggg accgggtgac ctttggcatc atggtggcgt
     2041 tgcctgtggc ccagctcatc atctttggct ttgccatcaa caccgaggta aaacatctgg
     2101 ccaccctggt gttcgaccag tcccggtccg ccgagagccg ggagatgcgg gacgcctttg
     2161 tggccacggg atatttcgac gtgttgcgcg aagtggagag cttttcggag attgaaaaag
     2221 ggcttcagag cggtgccgcc agcgtgggta tcgtttttcc tccggatttc gctccgcgcc
     2281 tccagcgacg tcagggagcc cccctccagg tggtggtgga cgcgtcggac tccatgtccg
     2341 caggcagcgc catttcggcg gcccagatgc tggggatggt caaggggcag gagctcatgg
     2401 cccgttgggg catgaccccc atgacggtct acgacctgcg gctgcgggcc tggtacaatc
     2461 cggacttcgt ttcccccttc tacatggtgc cgggcaccct gggcatgatt ctcaccatga
     2521 ccatggtcat gatcactgcg gcggcgctgg tgcgggagcg ggagcagggc accatggagc
     2581 agctcgtggt gacgccggtg cgccccctgg agatcatgct gggaaaagtt cttccctacg
     2641 tgatcgtggg gtacatccag ctttccctgg gcctcgcggt gggggtggtt ttttttgacg
     2701 tccccgtgcg gggaagcctg acccttctct acctcctgac cctgcccttc atcgccgcca
     2761 atctgttttt gggcattttg atctccaccg tggcccgcac ccagatgcag gccacccaga
     2821 tgtcattttt caccttcttg cccagcatcc tgctgtcggg ctttgtcttt ccccgggagg
     2881 ccatgcccga gattttcttt cagttcagcc gtattttacc cctcacgtac tttctgcgca
     2941 tcgcccgggg catcatgcta aaaggggcgg ggtgggacgc cttgggggga gacgcacggg
     3001 ctctgggagt gctcatcgcg atctttgcca cggtgagcct catggtcttc aggcggcggg
     3061 cggaatgacg cccccctttt actgacgcca atctttgcta tcatggcccg gggagagccc
     3121 cccgttattt ttgcgcgaga ggaggcgtca gatggccccc ctggagaccg gcaagaaaaa
     3181 gacgcgcaaa atgatcctca gcgtggtggt gggagcggac gtgaacgaag accgggtggt
     3241 ctccgccgag gtcttttcct cctctcagga ggcccgtcgc taccttcgct ttgtccggtc
     3301 gtcgcacccc cgggtgaagt acatggtcct gaggagcgaa ctcatggtgt cggaaggcga
     3361 gcccctggaa gtggggcgcc gcctttgggt cgtcatgctc tgcgatggcc ccaccccccg
     3421 ccagctctcg atcttttttc acgagctcca tgcggcccat cacgagatcc cccttaggca
     3481 gaagcacgtg gccaagttta ccggaaaaat catcgtatcg gcctggccct gcgagctcga
     3541 cggcgccgtc ctccctccgg gcccggagga aggatgaggg gcattgatcg caatcttttt
     3601 agtaaatgca agagtaaaaa aaatctcccc ttgcgccata ggaagcgatg ccctaggata
     3661 gacccggtct cgcccggggg aaatgggagg aggcggggcg ttttttccgc aattttaaaa
     3721 aagtatgcag cgcattggcg aagcgtcagt gcgaagaaag gggaggcgat gtggtatgtc
     3781 gggttgcaag tggcacgtgg cggcggagga ggtctttccg gctattgaac ggtggatgct
     3841 ccgggatggc tttgacatcg tgatagacat ggagaagtcc cagggtagca ccatagtgga
     3901 tgcctgcacg ggcgacgagt ggctcgattt ttacaccttt tttgcctcgt ctccctttgg
     3961 catgaaccat cccaagctcg ccagcgatga gttcaaagaa aagatcttcc ggtccgccat
     4021 caacaaggtg gccaactccg acatctacac ccgggagatg ggggagttcg tcaagacctt
     4081 cggagaggtg gcggtgcctc cgcccttcaa gcacctcttt ttcgtggact atggctccct
     4141 ggcggtggaa aacgccctca aggtagccat ggactggaag gtccggcgcc tcctggcctc
     4201 gggcaagatc tcccaaggag aggcctataa cggctcccga gggaccaaga tcctccactt
     4261 caacgaagcc tttcacggcc ggagcggtta caccctctcc ctcaccaaca ccaacgaccc
     4321 caacaaacac cagatgttcg ccaagttcac ggactggccc cgggtgctga atcccaagat
     4381 cacctggcct ctggagcgac atctggggga ggtggagtgg ctcgagcgca cggccatcaa
     4441 ccagatcgaa cgggccctgg ccaataaccc caacgacgtc tgtgccatca tcatcgaaac
     4501 catccagggc gaggggggag acaaccactt ccgctccgag tttctccagg ccctgcggca
     4561 tatctgcgac gcccacgacc tgctcctcat cttcgacgaa gtgcagtgcg gcatgggcat
     4621 cacggggaaa atgtgggcct tcgagcacca cggcgtgacc cccgacatct ttgcctttgg
     4681 caagaaggcc caggtttgcg gcatcgccgt gggtccccgg atcgaccagg tccccgacaa
     4741 ctgcttcgcc gtttccagcc gcatcaactc cacctggggg ggcaccgtcg ccgatatggt
     4801 gcgggccacg cgctatctgg agatctaccg ggacgagaag gtgttggatc acgtcgccac
     4861 agtgacgggc ccccggctga tgaaggggct ccaggagctc gaggccgagt ttcctcacct
     4921 catctccaac gtccggggta aggggctcat gtgcgcctac gatttcccct gtgagaagcg
     4981 gcgcaacgaa ttcaaggtgg gatgctggga gaaaaagatg ctcatcctcc cctgcggcaa
     5041 cgtctccatg cgctatcgtc ccgccctcaa cgtgccggtg gaggccatcg accgcagcat
     5101 ggagatctcc cgagaggtgt tgaagaaaat gggctaaccc ccctggaggg gcgtccatcg
     5161 aaggggaggg gccccgaagg cccctcccct tcggcttttc cccgggaggg ggctggcgga
     5221 ggatccgggg cgcgtgggag acgccccccc cctttttctc cgattgccga aaacgggaca
     5281 aaatgcgata ctctacattg aagagggggg aggatcttgg gaatcctggg ggagatgaac
     5341 tggagcctga tattttctct catcgccttg agcgccgtgg tggcctacgt gggcgaccgc
     5401 ttggggatgc gcatcggcaa gcggcgggtc tccgtcctgg gattgcgtcc caaacacacc
     5461 tcctcggtgg tgaccgcctt cacgggggtg ctcatcacgg tggtcaccct gggagtgctc
     5521 tcggcgacca gcgacacggt gcgcacggcc ctcttcagca tgaaaatggt gcagcgtcag
     5581 attgccgaac tcaccagcca gctgcaagat agccgggagg agtacgccga cctggaaaag
     5641 cgcctctttg acagccaagg ggagctcatc gaaaagcagc gccgcaccgg cgaggcggag
     5701 cgggccttgg cccagagcag cctggccctg aaggccatgg aagaacaggt ccagtcggcc
     5761 caggagcgcg cctcccgggc ggaagagatc cggcgggagc tggaacagga ccgggacaaa
     5821 ctccagacgg agagggccgc cctgcagcgg gacctcgagg gactccagaa ccaaagggcc
     5881 tccctggagc gcgagatcga agatacccgg cagcgcctga tgaaggtccg ggagggctgg
     5941 atcgtggccc gggccgggga ggtggtggcc cagggccccc tggaggccgg agccgatggg
     6001 gctgccctgc ggcgggccct ggaacacatg aaggcccggg cgcgcctttt ccttcaccgt
     6061 aacatgcccg ccggggagcc gacccccgag gtggtggtgc ccgagagggc cgaatcccag
     6121 gtggaatcct acctagaagc ccacgaaggg cagcgggtgg cgttgcgcct cgtggtgtcg
     6181 gccaacgccc tgcgcggcga agcggtccag gtgcgcctgg aacccctgga gagcaaaaag
     6241 atctacgaag cgggggcggt gttggccacg aagacctttc cggcctccat ggacaaaaac
     6301 gatgcggagc tcgccttgtc gatcttcctt cgcgacgtgg ggcaggaggc ggcggcccgg
     6361 ggagtcctgc gcaaccccct cacgggggac gtgggagaaa tcgatgcggg agacctggcg
     6421 gagggggtgc gacggctgac cgcctcggcg gagtccgtgg agatggcggt ggttaccttg
     6481 ggagagattt tcaccgaagg gcccctgagg gtcaaactcc gggtcccgga gtcctagggc
     6541 cgagatccaa aaaaacagga aggtggcgcg tgacggtgtt gattgtgtcg tgtcagaatt
     6601 gtggtgagat gaaaatcctg gccggggccc ccgatgcgga cggcctggcc cgggccatgt
     6661 ggttgtgccc ctgttgcggc gcggggcagg tggtacagct tccggtggcc gtggatgccc
     6721 gggggggcga cctgcgacgc atctgcggcg gcctctccgt gggggcccgg ggggagtacg
     6781 tctcggcgga agcctgtcat tagcctcgcc ccgccatgcc cctgggagag tatcgcttct
     6841 acgacagcaa aagggcggtg gatgccgagg gcctgcatgc cctgtatcgc tttacccagt
     6901 ggggaaggag ccgggcgctg gaggacatca gcctcatgct ggaaaatagc agcctgtgct
     6961 tttgcgccca cttcgagggg cgcgtggtgg ccttctgccg ggtgctcacg gacttcgtct
     7021 accgggcctc cctctgggac atcctcgtcc accccgacca ccaggggaag gggctgggga
     7081 cggctctttt ggattacgcc ctgcaccatc cggccctgcg ttccgtcccc gtggtgatga
     7141 cctatacccg agacctggcc cccttcctgg tccggctggg ttttcacccc gaagaggggg
     7201 ccctcatgct cctgaggcgc cccatcgagt actcctgacc tcccctgggg ggcgtcgcgg
     7261 cggaaagggg cggaagaaag aggaaaaagg atacaaagcc cgggacggtt tgacaccctt
     7321 gcccttaagg gatataaagg gtgtcgcttg gacttagcgt acgagccgtg gctaatcttc
     7381 ttccggacta agccgaaagg ggagcatttt ttttggaggt gctgtgtatg gcaggttgcc
     7441 cgacgaacca ccgttatctg atcagctcgg aatccgtcac cgagggacac cccgacaagc
     7501 tggcggatca gatctccgat ggcgtgctgg acgccatctt cgccgaggac cccatggggc
     7561 gggtggcgtg tgagaccttc gtcaccacgg ggctcgtgat ggtaggaggg gagatcacca
     7621 cctcctgcta tgtggacatt ccccggctgg cgcgttccat cgtgaaggaa ataggctaca
     7681 cccgggccaa gtacggcttc gatggagaca cctgtgcggt cctcaccgcc atcgacgagc
     7741 aatcccccga catcgcccag ggagtggata aggccctgga ggttcgggaa aacgacatgg
     7801 acgacgacgc catcgacaag atcggcgccg gagaccaggg catgatgatc ggcttcgcct
     7861 gcgacgaaac ccccgaattc ctccccatgc ccatcgccct ggcccatcgt ttggcccggc
     7921 gtctctccgt ggtgcgcaag gagtgcatct tgccctatct gcgcccggac ggtaagaccc
     7981 aggttaccct ggagtacgtc gacggcaagg cggtgcacgc cgataccatc gtggtctcct
     8041 cccagcacca cccctccgtg gaccacgccc agatcgccgc cgacctcacg gagcacgtca
     8101 tccgccccgt ggttcccgcc gagatctacg accatcgcac ccgggtgctg atcaacccca
     8161 cggggcgctt cgtcctagga gggcccatgg cggacacggg cctcacgggg cgtaagatca
     8221 tcgtggacac ctacggcggc gtggttcccc acggcggggg agccttttcc ggaaaagacc
     8281 ccaccaaggt ggatcgctcg gcggcctaca tggcccgcta cgccgccaag aacgtggtgg
     8341 cctcggggct ggcccagcgc tgccagatcc aggtggccta cgccatcggc gtggcccacc
     8401 ccgtatccat catggtggag accgcaggca ccggcgtggt ccccgacgaa aggctcacgg
     8461 agatggtgcg ggagcacttc gacttccgcc ccgccgccat catccgcgac ctcgacctcc
     8521 ggcgccccca gtaccgccgc atcgcggcct acggccatat gggacgcatc gatctggacc
     8581 ccatgcccgc ttgggagcgc acggacaagg ccgaagccct gagggccgcc gcccagtgac
     8641 cccagggata aaaatcgcct cgaagaacgg ctgacgtgcc cctgccctct ctcggtcccc
     8701 tgtgccggaa aaccctcgcc gttttccggc actttctttg gcccgccgtc tgcccctgct
     8761 gcggtgccct gggagagctc gcctgccctt catgcctgga tgccctggcg acgcacctcg
     8821 ggccccggtg ctcggcctgt ggcggcgcgt acccctgctt cccccattcc gccccacccc
     8881 tctggggccc cgtgccctac gggggcccgg cgcgggagct ggtccaccgc ctcaagttcc
     8941 ggagagagcg cgccctggga gccttgatgg gatcaagcct gggacggtgc cttccccctc
     9001 cggagggcgc cgccctcctg gtgcccgtac ccctgcaccg gggaagctcc cgggggtaca
     9061 accagtcgga agaaatcgcc cggggcctag gccgggagtg gagccttccc gtggtcgatg
     9121 cgttacaatg gacccaggac tgggatcccc agtcccgccg cgtgaaggcc tcccggcggg
     9181 acatcccctt ggaagccctt cgatgggagg gccccccccg ggggggagtg gtgctggtgg
     9241 acgacgtgtg caccacgggg accaccctgg atcgatgctc ccgggcggcg cggcgaggga
     9301 ggagctccgt ggtggcctgg gtagcttgga gtttgtcgga aggaggcata gcccatgcaa
     9361 cgcggggatt acccggtgag gaaatttgat ctgtgcgcct gtgtggtctg cggcaaggcc
     9421 tttgcctgtc gccccgagaa ccggaccctt tgccccgatt gccgccagga ggtggacgcc
     9481 ctgtacgaag acgtgaagga ctttctgcgg gcgaaccccc ggatggcccc cacggcggaa
     9541 cagttggcgg atcttttggg agtggaagag aaaaaaatcc aggccctgat ggccgatgga
     9601 cgcatcgccg aggtcgaaga ccctcgtcgc cgggatcgcc ggtgccaatc ctgcggagag
     9661 cgcatcaccg gggggaacct ctgcccccag tgcatggcaa aagtccatca aaataccctg
     9721 cgggcctccc ggaaagacct ctactctcgt cgatgaccca ccgtaaaacc ggggcagggg
     9781 cgctaaagcc ccccggggtc gtcgccgata ttatccgtga gggccatcga ggaaggccat
     9841 cgcagaaggc catcatgccg aggtgaggga tatgatcgag agaatccatg gcatcgccgg
     9901 aaccctaccc gcccaaaagg tgaaaaacgc ctaccgcccc ttctcctcgg gagagggacc
     9961 gggggatggc gtccaggtca gcgaattttc caagctcctt tcccgggcca cagcggaggc
    10021 ggaaaggctt cccgacgtgc ggtcggaaaa agtggagcag cttcgccagc aggtggaaag
    10081 cggaagttac gcgctgaagc tgggggtcgt ggcggcgaac ctggtggcgg cgggcatcct
    10141 gaaagacgag gtgtagcgcc ttggaggcgg ccctggtcga tgccatgggg cgtcagaccg
    10201 cagtcctgct tcagatgtgg gagatcgtgg tgcagcagcg ggaggcgctc aagtccgggc
    10261 gccttcctgt tttacaggac ctcatgcgct ccttgcaggg tatttccgcc caggcccgga
    10321 cggaagaagc ctctcgggac cgggaggccc ggcgcctggc ggcggccctg gggtgcgacc
    10381 cctgtgtgtc ggccctggcc gaggccctgg gggacggagg gggggggcgc cttcggggag
    10441 cggccgaggc ccttacccag gcggtgaagc aactccgggc ggagacccaa ctcctttccc
    10501 gcctggtgga agaaagtctg gcccttcagc agctcctcct ggcggaatac cggcgcttgg
    10561 aggggtcgat gacggggacc ctcccgaaca tggacttccg aggataggaa agaatttcac
    10621 gagagaccgc cgcccgcacc gcccccggcg gagggcccct ttaaggaggt tggagggatg
    10681 ctgaacagct ttttccccct ggagatggga cgccgggccc tgacctattt ccgccagggc
    10741 atggagaccg ccggacacaa catatccaat gccgccgtgg aggggtactc tcggcagcgg
    10801 gtggacgtgt ccgccaccga tcccttcacc gacccggggt tgaaccgccc cggcctcccg
    10861 ggccagatcg gcaccggggt aggggtggac gcggtccggc gcctccgcga cctgtttttg
    10921 gacacccagt acaaccagga gggcaccacc aaggggtact gggacaagat cgccgacgtg
    10981 ctccacacca tcgagctctt cgtccacgaa cccaacgccg acctggagtc ccccaccgaa
    11041 gtggcggggg cccaggggca ggggttcaag gccgccatgg attccttctg gtccgccgtg
    11101 caggaagtgg aaaaaatgcc cgatagcgcg gcaaaccggg aaaatctgct gcaaaaggcc
    11161 cagaccatga ccaccctcat gaaggaaatg gtggccaaca gcgaggagtt tcgcgcttcc
    11221 ctgaacaaag agctcaagct ccgggtggaa gaagtcaaca cccttctgga cgaagtgggg
    11281 gccctaaatc gcaccatcgc cgaagtggag aacgtggggt ggaaccccaa cgacctctac
    11341 gaccagcgcg acctaaaagt gacccgcctt taccagctca tcaactgctc cctcaagtgc
    11401 ccctgtgacc ccgacgacgg agacttccgt atctacctgg atggccggga gctggtccag
    11461 gggcaggaag tgcgtcacct caagctcgtc cccatggagg gcaacaaaag cttcttcgac
    11521 atccaggtgg aagacaacga gttcgacccc gtccgggacg gcgagggcgt cgaagtcatc
    11581 atagagcagc gggccgccga gggcatccac accatggaag tggcccgggt ggccagcgaa
    11641 acgcgctgga agatcggcaa gggcaacccc tccaccgaca cctatgtggg acgcattgag
    11701 cccccccgag gagacgaaaa cgcggccctg ggcatctcgg gatcctttcg cctctcggtg
    11761 ggcacctccg gcgtggaaag ccgctcccgc accctcccgg ggggcgtcct cctcaccgct
    11821 ccccccgtgg gcaaccagac caaagactac gccttccagg tgagctacaa ggacgtgagc
    11881 cgcgtcatcc aggtcaagtg ggacgaaagc ggggccctgg cgacccatca ctggaccatc
    11941 accgacggca ccatcaccca ccatgcggcg gcggggtccg acctgaaact ttcggatttc
    12001 gccagcaccg ggggcacgcc gggctttttc gacaagacct cgtcctttcc cgccctcact
    12061 gtggagtcca ccggaggagt gctccgtctg aaatcggcgg aggaccatct cctctccctg
    12121 gaagatatcc ggggagatct gctatcggga aagctccaga tggccaaccc ctcctccatc
    12181 ccggtgacca tcgccgtgac ggaagaagat tccctggcca ccatccgaaa caagatcaac
    12241 ggcgcctttg ccgccacgga aaatgccccc cggaggcccg aagaatggct ccacgcggcg
    12301 gtggaacagg acggcgaggg gcacacctac ctcaccctgg agagccacgc ggtgggggag
    12361 gcgtaccgga tcaacgtcct gggggacgcc aacggcagct tctacatggc caagcgtctg
    12421 ggcctggtgg atgccgacgt gaacccgccg cacatccccg gcggtgctca catcatggaa
    12481 agggccgaag acgccctttt tgccttcgac ggcaccaagt acctctcggc caccaacacc
    12541 ttccaggagg ctcggaagct ctcatcggaa gacggctacc gcgccgccac cctttccacc
    12601 gtgtccgagg gactccggtt tcgcatcgac cccaccaagt ggcgccggag cgagcccccc
    12661 accaccatcg acatacagca ccacgtcaag gggggggagc ttcgggggta catggaggcc
    12721 cgggacgacc gcatcttgtc ctatttggac tattttgaca ccctggtcta ccgcttcgtc
    12781 accgagacca acgccgtcca ctatgggggg cacggggcgg gggtcaactc cctggccacg
    12841 gggaatgccc tcttcgaccc cctcacgggc ccctccggag cggcaaggac ctttggggtc
    12901 agcgacgtca taatgaaaaa tcccagtctc ttggccgccg ccatcgacga cggccggggg
    12961 aggtcccggg gagaagggga tggggccaac gccctccgga tggcccagct caagcatgcc
    13021 aagatcatgg cctccggcac ctccaccttc aacgaacact acgaagcctt cgtggggcgc
    13081 ctgggagcgg agacccagcg ggccgattcc atgtcgagaa atcaaaagca cctggtggag
    13141 cagatcgacc ttcagcgcca gtccgtcatg ggggtcaaca tcgacgaaga gatgatggac
    13201 atcgtgcgct tccagcaggc ctttaacgcc atggcccggt tcatcaccac cacggacgag
    13261 atgctcgatc gcatcatcaa cggcctgggc accgtgggcc gatagggagg gatcgccatg
    13321 ccccgcatca ccaaccccat gatgacgaac atcgtgctgg gggacatgca caacaacctg
    13381 gcgcggatgc tcaagatgca gcaccagatc gccacgaaca aaaaatacga ccgccccagc
    13441 gacaaccccg tggacgtggt gcgagacctg tcgttgggga cgaacattta cgaaaattcc
    13501 caattcatcc gcaacatgga cgacggcatg acgtggctca aaaactccga aggggccttc
    13561 aaccacgtca cctccgtggc ccagcgcatc cgggagctgg ccatctacgc gggcaacggg
    13621 gccctggagg gggtggacat ggaagccatc gccacggaga tggccgagct ccaggaagag
    13681 ctgcgcaact ctgccaactt cagcgtggcg gggcgctatc tcctgggggg gctggacgtc
    13741 accgagtccc ccttcgtccg cgacgagaac ggatcggtgg tctaccgggg caacgagtac
    13801 cacgcggagt tcgaaatggg gcggggcgtc accggccagg tctccttcca tggccgggag
    13861 atcttccccg tgaacgagcg ccagtacgcc atccggggca ccgaagtccc cctggacttt
    13921 tcctggcagg gccgcccgga gatcctccaa tttcaggtgg gagaccgcat ggccaaggtc
    13981 cacctctcgg agctctgggg cgacgacaac tccaacggaa aggacgaacc cacggacttc
    14041 aaccagttcc gggatccggg ggagctccgg ggaaagaccc tcaaagagat cgccgaccac
    14101 atccaggcga gtctggccat gggggacgtg gggcgcctag tctccgtgga ggccaaaagc
    14161 gaccccaccc ggggggtcca gtggctcgaa atccgggggc acacgggaga gcccgtgcgg
    14221 gtgacctcct ggcccgagac tgacttcgcc aaacagcccg atgcggcctt ttccaacgaa
    14281 atggggcttg gaggggcgtg gaccgcagga agcgcgggcc ggatcaacat cgccttcgac
    14341 aacggcgagg tgggaagcgt cgatgtggcg cttgggaacg acttggacgc cgtggccaaa
    14401 agcatcaacg aaaacgccag cggcctatgg gcccaggtga aagaaaacgg aacccgccaa
    14461 accctggtgc tctacgccaa ggaccgggag cgcacctttc gggtttcggg cacggggggc
    14521 gcggaagagc tcttcgccac ctacgactac gcccatccca cgacgccggg ggtctacccc
    14581 gtgacagcga cggaaaagaa ctcccgccgg gtggatgcga ttccgggggc caccagcgaa
    14641 gccctggacc ctccagctgt gggggtgctg aatgacaccc tgaggttccg ttggaaggac
    14701 gggtatacga aaaccttgac cctggcgggg ggagaggaca tcgacgccgt ggccgccggt
    14761 atcaacgggc tctacgatcc cacagcggac cccacggacg cggggcatgc cctgtgggcc
    14821 gaagtgcgca ccacagagac cggggcccgg cagcttttcg tctacgccat cgacggaagc
    14881 atggggtttg aggtgtccgc cacaggggca tccacgacgc agtatttccc cgccactcgg
    14941 gaatcgacgg cgctctttcg aacgcaggag cccgaccaca gccatctgga tttttcttct
    15001 ctgatgcgcc tggagacctc ggtgaagagc gtagaagttc cggcggcgag cggcgtcctc
    15061 cgccccgccg ggggaggcga gctccacttc cacctggaaa gcggcggccg ccaaaccgaa
    15121 ctcatcatcg ccgacgacgg ggtgcctctt acgattgacg acctggccgc ccgtctccgg
    15181 ggcgtggcgg ggtcgtggct cgacgtcacc gtggaaagcg acgccccgga gggcaacgcc
    15241 tacgatcttc tcggccgggc cgacgccaac gccgagcccg ccaccaagcg ccttgtgctg
    15301 cgcagcaaag acggccaagg agtggcgatc ttcgaccaca accccgtacc gggcacgggg
    15361 accgcccctg tgccggacaa ccgcttcgct gctcagtggg gcctctccac ggccacctac
    15421 ggcgcggggg gagcggcgtt tcccgccggc accaccctgg atccctacat gcccgccctc
    15481 atggaggtct ccgtggggga caaggcctac caggtgaagc tctacgcctc gggggaagag
    15541 agcatcctca ccggcggcgt ggtggacgcc gccaagctgg cggcccagat ccagaagcag
    15601 gtgggatcgg acatcctggg gtatcacgtg gcgaacccca ccaacgccgc ctcggccttt
    15661 gccctctacg ccaagacggg ggagcccctg cgcatcgtcg atctcccctt cgccgacccc
    15721 aacgtggagg acttttccgg cgggctggcc cgctcggcgg ggatggccac gggggtcttg
    15781 gggggaggcg ttttggacac cgccacggcg ggggcggcgg ggctgatccg catccggacg
    15841 ggagccaaga ccgtcgagat ctccgtgcgg gccacggaca ccctgcgcga tttggctcac
    15901 cgcatcaaaa aagaagcggg agagtgggtg gacgtggcct actacgaccc ccgcgttccc
    15961 gacccgaatc atcccgccac gggggacatc gtgcagcttt ccctggcatc caaggacggc
    16021 tctcccctct ccgtgttcga cgtctcggga aggggcgcgg ccaccttccg gatagacacc
    16081 gccctgcgga gcaccgcaga tgtgtcgggg tgggcggcgg cggcggggga ccgcctcacg
    16141 ctgaaagtgg atggctacga acacaccatc gacctgtggg acgccaatgc catccctccc
    16201 gagcccctgg cggcggggaa aaaccttgac gggttggttt ccgccatcaa cgcccggttc
    16261 cagggcaaag acgtggtggc cgaggtcgtg gacgcgggag ttggggaccg gcggctcgtg
    16321 ctctcttccc cccggggata cgtggtggag gtggtggaag ccagaactca gctcacaaac
    16381 tccctgcacg tgcctcctgc ggccctgcgg agcaccgcga atgtgtcggg atggatggcg
    16441 gcggcgggag agtccctgga cctgaccgtg gacgggaccc tccacaccat caccctgacg
    16501 ggggcgacgg acctcaatgg cctggtggat gccatcaacg ctgaattcca gggaacgggc
    16561 gcggtggccg aaatcgtgga tgcgggagga ggtgttcggc atctggtgct ctcttctccc
    16621 caggggtaca cgatgacggc gaatgaagcg ggactggtgg ccccgctgca cctggcggcc
    16681 tccgtggctc ctccccagcg tcggggcggt cagggtccca tgaaccaggt caccacggtg
    16741 cgcactgcgg cgaatcagaa ggccacggac ttcttcggcg tcatggaaga tctggtgtcg
    16801 gcggtgcgcg aggaggatcg gcagggaatt tccggatcac tcctgaagcg catcgacacc
    16861 tttatggaca atttgctgaa gcgccgcacc caagcggggg ccatgctgct ccgctacgag
    16921 accagccagc ggcgcctcac ggacaacaac accaacctca cggatctccg gagcaaggtg
    16981 tcggagcccg acctcgccga ggccctgacg aagttcaaca tggcccaggc ggtctaccag
    17041 gcgagcctct ccaccatcgc ccggatcgtg cagcccaccc tggtggactt cctcaagtag
    17101 gccccaggcc ccattttttt tcggacaacg ccatggaagg gacgatgtga cgaccatgag
    17161 aaaaattgac acggttcgct tcggaacctt agaggtagaa aaagagagcc tcctccactt
    17221 cccgcagggc atcccgggct tttttcacct gaaagaatgg tttctcgtgg gagaggacga
    17281 caaccccatc aagtggctcc agagcgtcgg gaatggcgcc gtggccctgc ccgtagccct
    17341 gccccggagc gtgtgccccg agtacaaggc ccggatcgcc gaagaagacc tggaagacct
    17401 gggcgacgag ggggagatct gctccttcgt ggtggtaaac atcccctccg gggccccctg
    17461 gaagagcacc gtgaacctcc gggcccccat cgtcataaac catcgtaccc gccgagggcg
    17521 ccaggtaatc tccctcaacg aggaatactc catgagggcc cccctctttt cggaagaaga
    17581 acgcgaagtc ttggtggccc aagtcgcccc gggcgaagac ctcggggtcg cggaagaaaa
    17641 gtgacatgct cgtcctgacg cgcaaggtgg gggaatccct gctcatcggc gatgaagtcg
    17701 aagtcaccct ggtggaagtg cggggggatt cggtgaagtt gggcatccag gccccccgct
    17761 ccgtgggagt gtggcgcaag gaattggtgc aagaagtggc ccgggaaaac ctccaggcca
    17821 cccggggcga tgtgccgacc caggtgttgc gctggctggg gcgttccccc gatcgacctt
    17881 cggagaaaaa ataaaaacct taaggccccg ccccgctccc aagatccggt ggcgcgcgag
    17941 ccaggagcgc tcatcttcca gccggctctc tttaattttt tgagtcctgc attatcctct
    18001 aggctttacc cctacggccg tcacccgatg gggaatgtct cctcggcccc agcgacatgg
    18061 gggcggaagg tttttcgccc ctacaggttt cgcttgtctg ggaaaacccc tgggagtgcg
    18121 agccgggagc gcttatcttc cagccggctt tcttcaatgt ttttatttgt gcatcatctt
    18181 ctgggttttc gccccctgcg gataggccgg tggagcggag attggtgttc cctgggcgcg
    18241 cgagccggga gcgccagctt ctagctggcc ctcttcaggt tttttaattt ttttatcgtc
    18301 ttctgggttt tcgttcctac gcataggccg gtggagcgga gattggtgtt ccctactgat
    18361 ggtaaggatg tgaaccccca taggtttttt tgttaaaaac aaacagatgc cggctggaaa
    18421 atgcgcgctc ccggcaacgc gctctcggcc acgcccctgc cccttgagct ttatcttgag
    18481 tcttcctata atgttcccac ggcaccaccc ggaaaaacga caagcctctt gacagacatc
    18541 gcatcccgct gaggagaaaa aatgtcctga tgagatacga gccgaatcca aaacacaaag
    18601 agccgtggca gcctggtcgt aagggaacgc tctgtccccc agaaagcgaa atctctcttc
    18661 tggaagcagc gcgtcttttg cgagaagggt aaatagccga taaaaaatgt tataattttc
    18721 ataatggtaa ggcgtattgc gtgcgtcctc acggcgaaga cctctggcac ggttatcctg
    18781 tcccatggag tgaagttccg tacggtattc gggacaaatg gcggaagacc gggcgcgtga
    18841 cgcgacgcga aatgaaggag gatcgggaat gagcatgctt tttgttgagc taggagacaa
    18901 agagcgtttc gcggttcgga tagaactcct tcccgatccc gacaaaggac actacgcctc
    18961 tccggaggag gccctttcct gggggcgttt cgacctctgg gttcagggac gcaacctctg
    19021 cgccccccac ggcgtgacat ggtatctgct gccgcttttt gaatggttcg tccacaactg
    19081 gaacgccctc tttcacgaaa gcaggctccc cattttatcg gacgacgaga catggccatg
    19141 ggtgcggcgc gacgacgacg ccaggtcttt tccctactta agcgacgaag aagccgacag
    19201 gcgcgaggcc ctttggtacg aatggagcct ccgccatgcg tggcgttccg ccgccgaagg
    19261 aggccttttc cccgaccttc tcctcctccg ggagggtgat ttcgtgcggt tttcatgggg
    19321 tccgccgcct cctgtgggga cacccccggg gcccgttttt gagcatctgc ggggcgatgt
    19381 cctggtgccc ctcaatgccg tatgcacgcc cctctttgac gccctgcggg acatgtcgga
    19441 ttttatgatc cagcaggggg aagcgtgggc tcttgaggac ctttcccgct tggaagctct
    19501 gcggaagaac tttcaggagc tgcgcaccgt tgcgcccgaa gagcgcctga tctggctgag
    19561 cggcattgcg cgtaccctgg aaggcgctcg tcaaaaagtg gagatgctga aggatcgcct
    19621 gggggattct ttttctgctt tcgccctgga cggaatttca cagaacgaac ttgttctgga
    19681 aggttccagt ttggcgagca tgatgtacgg tagcacatct ccggaaatcg gcgagaagga
    19741 cgtcctcctc ttggcccgga gcgtcatgac gctgccccca tcggatgttg cgccaccccg
    19801 gatcccggcg acggatttct ccaggccctt tttggaaggc tacggcatcg cagaccgtct
    19861 tcatgatcat ctggggacag acatgacttg cccgtctccg atcgacatgg aggatgttct
    19921 tcgaaggctc ggagtttaca cggcttccct agatcttgat gacgagtgcg tcatgggagt
    19981 ggccgttctc cgctccgggt gtgcgccgtc gattcttgtg aatgagaagc atgagaaaaa
    20041 caggactcct cagggacgcc gcttcaccct tgcccatgaa ctctgtcacc ttctcatcga
    20101 ccgggagcac ggccgtcctc tggcggtggc ctccgggccc tgggctcccc gcgccgttga
    20161 gaagcgcgca aacgccttcg cggccatgtt cctgatgccc aaacccatgc tggattcgat
    20221 ggctgtggag tgttctgaaa acgccctggt cgatgcggtg gcggtgaaac tgaaaacggg
    20281 aaaacttgcc accaagtggc atctgagaaa tttgggtttt ctgcccgaag acgtagagtc
    20341 ggtgtaaggc ttcgagaaag tgggggaatt gcggtgactg tgccgattcc ccatgcggcg
    20401 tatgctcctt catggaccgg aaaacttccc gacaccgcca cgctcccggg cacgatgtgc
    20461 cgaaaaatcc cattcgggcg atgtgcttca gcgaacggtc ctttccttcg aatctttaag
    20521 gagggaacct cgtttgcctg atgtgtcgca cagggagggg ggtttcccct cttgcaagat
    20581 acaccttcgt acaaccccgg gctccggact aactcgttcc tgctcttacc atacggcatg
    20641 gtcacgctgc tggcggcgac ggggttcttc actccgctcg acggggtgct ctccctcgtc
    20701 atgtgcatga tgccctcgat gaaccacagc agcgtcatct tctcctcgtg gctccactgg
    20761 cgccggtcca gttcggcaag aagaatcttc atgtaatcca gcttccgggc gtcgttcccc
    20821 cgctctttcc acgcctgcat cccggcgtag tgcgccaggt cgaaggggtt gtcgctctcc
    20881 cgcagggccc ggtcgtcccc ctcgtagatc ttgacgacgg gataccggta aatcacctcg
    20941 cagtcgtatt cctcccaatg gtacgccccc tggccctgct ccgccgggat ttgctggacg
    21001 atgatggcca tccccaccac ggagcggtgg taccgtcctt cgagcaggca gcggtaccgg
    21061 tgcatccgca gggagaagtc cccccggccc ccgcgccccg ggatctccac gtggagcaac
    21121 acccacgcac catctgtggc acgggagggt gcgtagccat agccatgcag cactaaatga
    21181 agtaggggcg aaaaatatgc tcgcccctac gggtttaccc cggcggggaa aacctccggg
    21241 cgtgcctctg gctgatgccg actgagaact gggaattggt atatggtctg cgaa
//
LOCUS       DAOOER010000034        18984 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_3356_length_18984_cov_8.556501, whole genome shotgun sequence.
ACCESSION   DAOOER010000034 DAOOER010000000
VERSION     DAOOER010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 18984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18984
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3356_length_18984_cov_8.556501"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PL162_05920"
     CDS             complement(<1..809)
                     /locus_tag="PL162_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91595.1"
                     /translation="MLAELLRNGDMVLLPEKHRPEGIPAPIVPYVVLLAPYNRALVGT
                     DVLGCHERWLWGKLGGDLWQDVTLYDDKSKTLSLGIFGDIRVNSQAVTLLRRELLSHL
                     LPPGDHEAALRLLREALRHDAVASIVEKSQDPTLWGELVEEDETLRMAYWGFRLALAW
                     GTSETIVHLRTWLRLARHSFDGSLRKPRLWAFLGSPDEGSLKDLMALGFREEPLHALS
                     SNEISPAAVKGPGGYLTQFWYHQGSPKDGVPPLGMRLWLYLTSALWDDLRGR"
     gene            1052..1243
                     /locus_tag="PL162_05925"
     CDS             1052..1243
                     /locus_tag="PL162_05925"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016862 - intramolecular oxidoreductase
                     activity, interconverting keto- and enol-groups [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxymuconate tautomerase"
                     /protein_id="HQF91596.1"
                     /translation="MPIIQIHLLEGRSTEQKRDLVRCVTASTCDALQVRPEQVRIILS
                     EMSQEDYAIAGVLMRDRSS"
     gene            complement(1259..2110)
                     /locus_tag="PL162_05930"
     CDS             complement(1259..2110)
                     /locus_tag="PL162_05930"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQF91597.1"
                     /translation="MNPSLASALFSGLRSFSAPGWRASALAWLLGWGLRRAKPRKKVA
                     LDNLRRAFPEKDEAWRVAVVDEIYWHLAWSLAEYGALLKDPPQALAWCVPDEGVDFLD
                     RCAAEGRGAVICAGHCGNWELLGAWFARKSYPFAAIVRRHDDLLMEELIDGARKTVGF
                     STLGKDEPMRRVVRRLKEGGFVAVLIDQRAGSEGIEVPFFGVPATTFPGAAALSRLAQ
                     VPMVPVFSFREKPFKHKIYVGAPLSCAAGSRDEEIGALMTEANLVLEKLIRRYPAQWL
                     WLHRRWP"
     gene            complement(2107..2430)
                     /locus_tag="PL162_05935"
     CDS             complement(2107..2430)
                     /locus_tag="PL162_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91598.1"
                     /translation="MLVLPALPEVSDGWLAAHGALMAVPVVAAPSPVLDDLLGHGGYI
                     PVDLSRGATVEAWIGALDRVMSSEGRAAAARARQHLELSFSPSSSGEELAALYRRVCG
                     AEVQR"
     gene            complement(2446..3039)
                     /locus_tag="PL162_05940"
     CDS             complement(2446..3039)
                     /locus_tag="PL162_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91599.1"
                     /translation="MGVQVTWFIPRDVLPWEVYVLRSLAGGLKGHDVTLRVLSQHPGS
                     WGTMPVLYWDAARVGERWRHLLRPGALWHFWGGAPSWHALVAFRAKTIHTQWRTTGPW
                     RGLASSFSSQNAREGEVRLWPAFDKQLLWNNGGDAPSVRCGEGGLSVLVPPRRRWVKG
                     VRDALRLRGDAVQWIPWGPRPEDFGEELPLSEALYAH"
     gene            complement(3136..4746)
                     /locus_tag="PL162_05945"
     CDS             complement(3136..4746)
                     /locus_tag="PL162_05945"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQF91600.1"
                     /translation="MRLRILGAAGEVTGSCYLLENGDSRVLVDFGLHQGRNDDDRNRE
                     PFPVVPQSLTAVVLTHAHLDHTGRVPLLVKGGYGGKVWCTLPTAELTDVLWRDAARLM
                     QEEAEWKTRKNRRKGLEDVEPLYSAEDVDQALRRLAPVGYDQLLEVAPNIRVRFRDAG
                     HILGSSILEFWMIDGAQEVKVVFSGDLGPQETVMEKSPACIEDADYVVIESTYGDRLH
                     KTNLESRDEFRDVLRQALKGRGKVLIPTFVVDRAQRVLYELMLLRQEGDLPPDVPVFF
                     DSPMGVRATEIYRRHTDLLSSEIQERIHKGIDPFALENLQYVAEPEASREINDVRHAL
                     VMAGSGMCTGGRIVHHLKHNLWNESCHVIFVGYQAQGTLGRRLVDGDKILRIAGEDVT
                     VKAQLHTINGFSAHADRRDLLRWARNFRNGPLFIVTHGESKSSQALAEGLKEEGFRAE
                     VPAVGQEFSLESGQSFSPAVIVSEAFAERSRRTIQAQILGDIADLALSIRESGDSISD
                     EMAGLLQTSRMLLRLVRQQSAQEEEKAG"
     gene            4943..5533
                     /locus_tag="PL162_05950"
     CDS             4943..5533
                     /locus_tag="PL162_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HQF91601.1"
                     /translation="MDPGRRESATRRVGEMVSLTYLASLDLAGMGLIFCARVLDVSIG
                     TFRLLLLVRGKRFFAASLGFVEVMVYMLVLGSILGGGKSLDFPHLLAYCAGYATGNYL
                     GSLLEETLMRGFVLLELIADGDDEDLAVVEQLRSAGFGATVIRGEGKCGGRCIVKVVC
                     SRKDVDSASLIIGNRGFVVLSDVRGVWRGYFRGVRK"
     gene            5765..5842
                     /locus_tag="PL162_05955"
     tRNA            5765..5842
                     /locus_tag="PL162_05955"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5800..5802,aa:Pro,seq:ggg)
     gene            5901..6740
                     /gene="rsmA"
                     /locus_tag="PL162_05960"
     CDS             5901..6740
                     /gene="rsmA"
                     /locus_tag="PL162_05960"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HQF91602.1"
                     /translation="MPRGIPFSPRTEIGQNFLVDRNLPRIIVEDAGICPGETVLEIGP
                     GKGVLTKALLDSPCGRLDAVELDRRLAPFLEPYQKNEPQRFFLHWGDAVRFDYSSLPP
                     PTGVVANIPYHITTPLIWKILEDLAPQGLSRLVLMIQKEAALRLAAPPRSKARYPLGV
                     TLELMGGVQILRPVPPEAFRPIPRVDSALVRIDLKAHRDLPAIASWRELLRRAFGERR
                     KMLRNSLEGLPLPLACPSWETCLEALGLPTTSRPEELDGEAWIELWRSLEGTKRPSGA
                     PSA"
     gene            complement(6903..7172)
                     /locus_tag="PL162_05965"
                     /pseudo
     CDS             complement(6903..7172)
                     /locus_tag="PL162_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300848.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
     gene            complement(7262..8602)
                     /gene="der"
                     /locus_tag="PL162_05970"
     CDS             complement(7262..8602)
                     /gene="der"
                     /locus_tag="PL162_05970"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HQF91603.1"
                     /translation="MAIVTIVGRPNVGKSSLFNRLIGRRLAIVDDVPGVTRDRLYGEA
                     CWQDRRFYVVDTGGIVTGSDEPLMEGIVRQVRLSLEESDAVLFVIDGREGATPLDQDI
                     AQVLRKSARCPVILVANKADDPRHEDEAQGAYSLGFDCLVAVSAEHRRNIGELMEIVV
                     ALLPPEELPPEDLSDELRVAIIGRPNVGKSSIFNALAGEERSLVSPIPGTTRDAVDSL
                     VEIDGTPFRFVDTAGLRRRSRISSDVEYYSMLRSYEAIDRCAVAVLVIEGGEPATDQE
                     KRLAGHVLERGRGLVLVVNKWDLAPREESLGDRMRKYLRREFAFVDHAPTAFVSAKTG
                     RGLQKIPQHVLQVYENWRRRIPTGELNRILRDLMAFERLPSDGAGKFLRIYYASQVRT
                     APPSFAFFVNDPDIVTKGFSRYVEKRLRGVALFDGAPMRLYWRTGERKSAHGAP"
     gene            complement(8642..10429)
                     /locus_tag="PL162_05975"
     CDS             complement(8642..10429)
                     /locus_tag="PL162_05975"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91604.1"
                     /translation="MEITLEVCVPGPWWHALTYRFDRPLPPGVRVKVPVGRGHRVGFV
                     APDGPSPPPGQGELKEIEDVLDGAPPLGEELGRLASWIGINYLCGTPLALGRIAPGFL
                     LRGQGAFQEDLPVRASSGDSEDAFVFDPRDDRRWGRYREEIGGVSGGILALFGEQSDA
                     ARFWDLLPDRERGRGILWPSSGGRALQRAWEAVRSGERSLVVGGRGALFAPLRDLRLV
                     VVDEEASGGHRFVDPPFLHARTVASQRARLWRSRFLAGGRMPSARLFRMEGGRGSERP
                     GDRLFFVDLRRTDAVAVAGVRDPLRIGAATFRETKRAVQRGMGALWILDRKGYASEVA
                     CAECGAPLRCPSCGGTCRWERGRYACPFCGTPQAQDPSCSVCRGILLQGLRPGIETLW
                     EAASDLFRGDFPVIFWAGGKRGTLEARRALRKSGGVVLGTRGALALCDHLSVGCVAWI
                     DADAEARQPRYDARFVAFRMIWESLWRGGRCEDRTVVVQSRSPLRGWQESLPRGWGAF
                     WAREMEERRLYNLPPYRPLLSLEAGLEDKEALRKALESQGVEVLDPGLGDGMLWARPE
                     HLGAARRALEPFFRIRRKGPFPRIVLERE"
     gene            complement(10429..11646)
                     /gene="coaBC"
                     /locus_tag="PL162_05980"
     CDS             complement(10429..11646)
                     /gene="coaBC"
                     /locus_tag="PL162_05980"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HQF91605.1"
                     /translation="MSWWKNNRRVLLGISGGIAAYKAPEIVRGLRRTGCEVDIILTEA
                     AESFVSPLVLGTLSGRRVWRQKDFLSPAGGAQIPHITLAGRAETLCVAPATANTLRRA
                     ARGEAETLLGAAILATRAPVVLFPAMNEHMWDHPTTRRSVEECRAWGYGVVEPEEGAL
                     ACGYEGRGRLPSVEVILEEIWRRLRPLPDLVGRKVVITAGPTWEFLDPVRFISNPSSG
                     RMGFALARTAWYRGAQVCLISGPVALSPLHGVRHISVTSAEEMRRAVLAEMDDAAVIV
                     KAAAVGDYRGASPSSQKIKREGQALRTIDLVANPDIAAEVGSRKRPHQILVGFAAETE
                     DLRAHAEEKMRRKNLDFIAANLVSGEEGAFRRETNRVELLSPWAEDQVLAGSKDEVAD
                     GIWDAVVQRLSQV"
     gene            complement(11627..11881)
                     /gene="rpoZ"
                     /locus_tag="PL162_05985"
     CDS             complement(11627..11881)
                     /gene="rpoZ"
                     /locus_tag="PL162_05985"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HQF91606.1"
                     /translation="MIFSDLEELCEKFDIESKYVLTVIVSQRARFLSEQKGRYVAGDS
                     SEKYISTALQELVDGKLRHSCPAVERKAVPGVPEHVVVEE"
     gene            complement(11887..12474)
                     /gene="gmk"
                     /locus_tag="PL162_05990"
     CDS             complement(11887..12474)
                     /gene="gmk"
                     /locus_tag="PL162_05990"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HQF91607.1"
                     /translation="MATAKNRGRVIVLSGPSGAGKGTLRKRLFEKIPELVFSISCTTR
                     PPRAGEQDGVDYWFVSEEDFSLWRDQEAFLEWAEVHGHHYGTRWADVERGLEAGKCVV
                     LEIDVQGARRVKALFPDALLIFIMPPSSEALQDRLRHRGSEGEDTLVLRMENARREME
                     QASFYDQVVVNDDLEKALDCLVPAVRACVDGIKGR"
     gene            complement(12455..12724)
                     /locus_tag="PL162_05995"
     CDS             complement(12455..12724)
                     /locus_tag="PL162_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HQF91608.1"
                     /translation="MQYRLLHVGFGNMVVSDRVVGIISPSSAPIKRLKEEARGAGLLV
                     DATQGRKTRSVLIMDSNHVILSALQPETLAHRFVEEEEGDGDGEE"
     gene            complement(12731..13615)
                     /locus_tag="PL162_06000"
     CDS             complement(12731..13615)
                     /locus_tag="PL162_06000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HQF91609.1"
                     /translation="MLISMTGFGRCQKSATWGNLSVEISSVNHRFQEVSLRLPRELGG
                     WEIPLDRQIRQSFNRGKAEVRVAVSWAPTLRAASVREDVLEKYVRSVAAVGEKLRFPR
                     SGELESYLGLPGVLETGEPQEKVREQLEFDLQETLKAAMESWQAMRRNEGKHMEEDLL
                     GHLASYRGRLDEIRSLWPGARDEALRSMGTRLQALLDQGGWRLDEARFAQEAAVLADR
                     WDVAEELSRSESHMAQFRSVMAEDGGVGRKLDFLVQEMNREINTMGSKVSDGRIRWLV
                     VEAKADLERMREQIQNVE"
     gene            complement(13609..14763)
                     /gene="hflX"
                     /locus_tag="PL162_06005"
     CDS             complement(13609..14763)
                     /gene="hflX"
                     /locus_tag="PL162_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HQF91610.1"
                     /translation="MKSSRAVTLFVQQKNQTDEEVALLQGELSLLLGNMGVTVGASVV
                     QRRDAPDPAYYIGQGKALEAAAAAQAVGAKMVVCDDVLSPSQLFHLSQLTKLEVWDRA
                     RVIMRLFDARAHSAEAKLQIDLARCRMELPSLKGLGQQMSRTGGGIGTRGPGETEFER
                     HRRKFHRRERDIMKRLDLVRQRRQGQRERRRRQGFFTVALVGYTNSGKSTLLRSLSKD
                     PSIFVADKLFATLDTALRPVFLPSGERVMVADTVGFVRKLPHDLVAAFRATLEEVRES
                     DLLLIVLDLTAQDIQGDLEVVQSTLKDLGAGALPRIIVLNKEDLAPPEARESLLRRWS
                     EEGETVRAVSALRGTDLKSLCRAVDDVLFGGGVRGERSLGPFGEDGGSTC"
     gene            complement(14760..15407)
                     /locus_tag="PL162_06010"
     CDS             complement(14760..15407)
                     /locus_tag="PL162_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQF91611.1"
                     /translation="MHRGPYRLFYVLTCGVLRAFLLCFHRFRVRGQDSLGLPRRVIVV
                     ANHCSNLDPMVVGVSFPWTLRYLAKTELFRAPLFGFLIRNLGAIAVEREDSQRAGAVL
                     KLLLDLLEEGQSVLLFPEGHRSRDGFLQPLEGGFALLSLKMGVPVVPAVISGTFAALP
                     PGGKWRWKPVSICYGPPIDPASIPSEMPDKTRRRLLVERVEGALRSMGAKGAGSP"
     gene            complement(15400..16071)
                     /gene="cmk"
                     /locus_tag="PL162_06015"
     CDS             complement(15400..16071)
                     /gene="cmk"
                     /locus_tag="PL162_06015"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HQF91612.1"
                     /translation="MSMTPVVTLDGPAGAGKSTVAKRLAARLGLSYLDTGALYRALAF
                     VLDRGGVLPAEGPELLRALEALHVDLREGRVWVGSEDVTEFLRSSRVDSVVSSYAALP
                     PVRDRLLDLQRQQARSGGLVAEGRDMGTVVFPRASLKIFLTASDEERARRRFEERRLR
                     GEEVSMEVVREGIRHRDGQDASREVAPLRPAENAVLVATDGKTVDEVVEEIVTLFRKA
                     DCSSA"
     gene            complement(16071..16952)
                     /locus_tag="PL162_06020"
     CDS             complement(16071..16952)
                     /locus_tag="PL162_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HQF91613.1"
                     /translation="MQGLDERTRDLIQRRVLKRVKEIPSLPQFVVETLKKLDDPKSSA
                     ADVSNRLSRDEGLVIRILRLANSAYYGLPRRIVSVTEAIALLGFKTVKSIVLSASVYQ
                     FMDGAFTGYALDRGQLWKHSLSVAFVARHIAKKVKGEEEEAYVAGMLHDLGKIVLNDF
                     VRFGYGIIVRLVEDEYMPFMDAETQVLGFNHAQVGGLMLEQWNLPETYQYAARFHHNP
                     EECQDVPQTIRRMVDVVHVANAVCLMMGLGIGADGLQYTLSEAAMERLRLESIEGLMS
                     EVVDFISQVDEEFRLGD"
     gene            complement(17002..17721)
                     /locus_tag="PL162_06025"
     CDS             complement(17002..17721)
                     /locus_tag="PL162_06025"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HQF91614.1"
                     /translation="MESAGSVRLNKYLASCALGSRRHVEDLVHQGRVEVDGKRALSPG
                     ERVGPGGRVRVDGRVVMPRELCYVVCYKPRGVVCAVWDRFYPTVLTVLPPKFQDRGLF
                     PVGRLDKDSEGLLILTNDGDFAQSVAHPRFGVTKKYRAELSRPMDAEDLGRWRRGVDL
                     EGRWVVPEGVDRFSGGVEVTLREGMKREVRMMARALGYEVTRLIRVAIGALKWEKLPP
                     GGWMTFSREELWCMIRAGGLV"
     gene            complement(17721..18302)
                     /gene="scpB"
                     /locus_tag="PL162_06030"
     CDS             complement(17721..18302)
                     /gene="scpB"
                     /locus_tag="PL162_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HQF91615.1"
                     /translation="MNGVRGEAPLQNPSIRTLEALLFVATQPVPSASLAAVLGISSRE
                     VGRILSQLREHYEAGHGVTLIEVAGGWQMVTAPEEEEAVRAFRADHLDEKIRLSKASL
                     ETLAVVAYRQPVTRGEVEEIRGVRCDRVMETLLRHGLVRVAGRRRGSGTPLLFRTTDR
                     FLELFGLQAIADLPALEELREALPEAFSEEGMA"
     gene            complement(18299..>18984)
                     /locus_tag="PL162_06035"
     CDS             complement(18299..>18984)
                     /locus_tag="PL162_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91616.1"
                     /translation="DVVRLYGAYLARGDVMTIREMAEFFALAARLLLGKLQALMPGIF
                     REEEGGAPEEDPTGTVGGTEEDVEEALARYRPFRAAARMMEERFHRRAAYVGRRAEEG
                     EKIFDLGDVYALACLWWPLIRQKRRPSVGICEEDFWDEEGVPSMVPEESQVESCMDRL
                     RSLMTPHVPVELDGLLEERRSRSALVLVLLALLELSRRNEVKLVQSDCFGSLRVTRCA
                     NPAVEGSVP"
BASE COUNT         3703 a         6383 c         5321 g         3577 t
ORIGIN      
        1 cgcccccgga ggtcgtccca aagggccgat gtaagataga gccagaggcg catccccaag
       61 ggaggcacgc cgtctttggg ggagccttga tggtaccaga actgggtcag gtatcccccg
      121 gggcccttta ccgccgccgg acttatctcg ttggagctca gggcgtgcag gggctcctcc
      181 cgaaagccca aggccatcag gtccttcagt gatccctcat caggactccc caggaaggcc
      241 catagccggg gttttcgtaa cgatccgtcg aaggaatgac gcgcaagacg aagccacgtg
      301 cggagatgga cgatggtttc cgatgtcccc caggctagcg ccaggcgaaa gccccagtag
      361 gccatccgca aggtctcgtc ttcttccacc aattcgcccc agagggtggg gtcttgagat
      421 ttctccacga tggaggctac ggcgtcgtgg cgcagagctt cgcgtaagag tcttagcgcc
      481 gcctcgtggt cccccggggg gagaaggtgc gacaaaagtt ctcggcgcag aagggtcacg
      541 gcctgggaat tgactcgaat gtcaccaaag atgcccagag atagggtctt actcttgtcg
      601 tcgtacaggg tgacatcctg ccaaagatca cctccgagtt tgccccaaag ccatcgttca
      661 tggcatccga gcacgtccgt tcccaccaga gccctgttgt aaggggctaa gagcaccacg
      721 tagggaacga tcggcgcagg aataccctcc gggcgatgtt tttccggcag aagaaccata
      781 tccccattgc gcaacagttc cgccagcatc gcccaccctc ccttcatcca ttcattctcc
      841 cctcgcgggc tttcgagggg gccttcttat gttgttgttc gatcacaata gggccgccga
      901 cgaaataacc tatgagaact acccctttcg gggctagtga cccacccgtg acctggcccc
      961 gtcacgcctt ccgcgacaga cctggtataa tccagccatc gaagccccct taccccgggg
     1021 tcccgaagcc cacacgaagg gagggttatc catgcccata atccaaatcc atttgctgga
     1081 aggccggtcc acagaacaaa aacgagacct cgtccggtgc gtaacggcct cgacgtgcga
     1141 cgcgcttcag gtccggccgg agcaggtgcg catcatccta tcggagatgt cccaggagga
     1201 ctatgccatc gccggagtgc tcatgagaga ccgttcttca tgattcccta tcgccgcatc
     1261 agggccagcg gcgatggagc caaagccact gggcgggata ccttctgatg agcttctcca
     1321 gtaccagatt ggcttccgtc atgagagcgc cgatctcctc gtcccgacta cctgcggcgc
     1381 acgacagggg agcccccacg tagatcttgt gcttaaaggg cttttcccga aaggaaaaga
     1441 ccggcaccat gggcacctga gccagacgag agagggcggc ggccccgggg aaggtggtgg
     1501 cgggaacccc aaaaaagggc acctcaatgc cctcggatcc cgcccgctgg tcaatcagca
     1561 ccgccacaaa gcccccttct ttcaggcggc gcaccactcg acgcatgggc tcgtcctttc
     1621 ccaacgtgga gaagcccacc gtttttcggg ctccgtcgat gagctcttcc atcaggaggt
     1681 cgtcatgacg gcgtacgatg gcggcaaagg ggtagctctt gcgggcgaac caggccccta
     1741 ggagctccca attgccgcag tgtcccgcac agattaccgc cccccgtcct tccgccgcgc
     1801 aacgatccaa aaaatctacc ccttcgtcgg ggacacacca tgccagagcc tggggaggat
     1861 ctttcagcaa ggcgccatat tcggccaggg accaggcgag gtgccaatag atttcatcca
     1921 ccacggcaac gcgccaggcc tcgtcctttt ccggaaaagc ccggcgcaag ttgtccaggg
     1981 ccaccttctt tcgcggcttg gcgcgccgca atccccaccc cagtagccaa gccagggcac
     2041 ttgccctcca ccctggagcg gaaaaggagc gaaggccgga aaagagggcc gaggccagcg
     2101 atggattcat ctttggactt ccgcgccgca gacccgacga taaagcgccg ccagttcttc
     2161 cccagaggag ctgggggaga aggacaactc cagatgttgc cgggcccggg cggcggcggc
     2221 ccgcccttcg ctgctcatca ctcggtccag ggcaccgatc caagcttcga cggtggctcc
     2281 ccgagagagg tccacgggga tgtacccccc atgaccgagg agatcgtcca ataccggcga
     2341 gggggccgcc accacgggaa ccgccatgag ggctccgtgg gccgctagcc atccgtccga
     2401 gacctcggga agggcgggaa gtaccagcac ccctccccgc tctcgctagt gggcgtatag
     2461 ggcttcggag aggggaagtt cttccccgaa gtcttcgggg cgaggtcccc aggggatcca
     2521 ttgcaccgcg tcgcctcgaa gccttaaggc gtcgcggact cctttgaccc agcgacgcct
     2581 cgggggcacc aggacgctca agcccccctc gccacaacgc accgaagggg catctccccc
     2641 gttgttccac aggagttgct tgtcgaaggc gggccacagc cgcacttccc cctcccgggc
     2701 gttctgagat gaaaaagacg acgccaggcc ccgccagggc cccgtggtgc gccactgggt
     2761 gtggatggtt ttggcccgaa acgccaccag ggcatgccag gaaggggcgc ctccccaaaa
     2821 gtgccatagc gccccgggac gcagaaggtg gcgccaacgt tcccccactc gagccgcatc
     2881 ccagtagagc acgggcatag ttccccacga tccggggtgc tgggagagga cgcgcagtgt
     2941 tacgtcatgt cctttaagtc ctccagccag gctccgaagt acgtacactt cccaggggag
     3001 cacgtcccgg ggaataaacc aagtaacctg gacgcccact gcattccctc ccttcgccta
     3061 aacgccacaa accgaccacc gagacgcccg gcgtagttcc gggaccctcc gaacccagcg
     3121 gagaaaaaaa tcgttttatc cggccttttc ttcctcctgg gcgctttgtt gtcggaccag
     3181 gcgcaacagc atgcgagagg tctggagcag cccggccatc tcatcgctga tagagtcccc
     3241 gctctcccgg atggagaggg ccaagtcggc gatatccccc aaaatctggg cctgaatcgt
     3301 gcgccgcgac cgctcggcga aggcttcgga cacgatgacc gccggagaaa agctctgacc
     3361 cgactccaag gagaactcct gccccaccgc agggacttcg gcccggaacc cctcttcttt
     3421 tagcccctcc gccagggctt gggaggattt ggactctccg tgggtcacga tgaaaagggg
     3481 accgttgcgg aaattcctcg cccatcgcaa gaggtctcga cggtccgcat gggcggaaaa
     3541 cccgttgatc gtgtgcagct gggccttgac cgtgacatcc tcgcccgcga tgcgaagaat
     3601 cttatctccg tccaccagcc ggcgccccaa ggtcccttgg gcctggtatc ccacgaaaat
     3661 gacgtggcac gactcgttcc agaggttgtg cttgagatgg tgcacgatgc gtcccccggt
     3721 acacatgcca ctccccgcca tcaccaaggc gtggcgcacg tcgttgatct cccgagaggc
     3781 ttccggctcc gccacatact gcaaattctc caaggcgaag gggtctattc ctttgtggat
     3841 ccgctcttgg atctcggagg acaagaggtc cgtgtgcctc cgatagatct ccgtggcccg
     3901 tactcccatg ggggagtcga aaaacaccgg cacatccgga gggaggtctc cctcctgtcg
     3961 aaggagcatg agctcgtaga gcacccgctg ggcccggtcc accacaaagg tggggatgag
     4021 gaccttgccc cgacctttca gggcttggcg cagtacgtca cgaaattcgt cccggctttc
     4081 caggttggtc ttatgaagcc gatctccgta ggtggactca atgaccacgt agtccgcatc
     4141 ttctatgcag gccggagatt tttccatcac cgtttcctga ggtcccaggt caccggaaaa
     4201 gaccactttg acctcttggg caccgtcgat catccagaac tccaaaatgg agcttcccag
     4261 gatgtgcccc gcgtcgcgga aacggacccg gatgttcggc gctacctcca ggagctggtc
     4321 gtatcccacg ggggcgaggc gtctcagggc ctggtccacg tcttcggcgc tgtaaagggg
     4381 ttccacgtcc tccaatccct tgcgacggtt ctttctggtc ttccactccg cctcttcctg
     4441 catgagtcga gccgcgtcgc gccagaggac gtcggtcagc tctgccgtgg gaagcgtgca
     4501 ccaaactttg ccgccatagc cgccttttac caacaacggc acccggcccg tgtggtccaa
     4561 atgggcgtgg gtaagcacca ccgccgtgag gctctggggc acgacgggga aaggctctct
     4621 gtttcgatcg tcgtcgttac gtccttggtg caggccaaaa tccaccagca cccggctgtc
     4681 gccattttct aggagatagc acgatcctgt cacttctccc gccgctccca agatgcgtag
     4741 cctcatgccc cgtcgccctc gcggccgtga ttcgatacgc cagttcacga gaaccgcgag
     4801 ggcttccccc cttcccgact gtaggagtct ttcgccctta ccattgtagc ccgtgccctt
     4861 cgccttggga acatcccctt tacagaaaga gccccgcgtg aggtatactt tccctgcgct
     4921 tcctcgggga gaaggaaatc cgatggatcc aggccgtagg gaaagcgcca caagacgcgt
     4981 aggggagatg gtttctctga cttacttggc atcgctggat ctggcaggca tggggctgat
     5041 cttttgtgcc cgcgtcctgg acgtgagtat cggcaccttt cgcctccttc ttttggttcg
     5101 ggggaagcgg ttttttgccg catccctcgg cttcgtggaa gtcatggtat acatgctcgt
     5161 gctggggtcc attctcggag ggggaaagag cttggacttc cctcatttgc tcgcctactg
     5221 tgcgggatat gccacaggaa attacctggg atccctgctt gaagagaccc tgatgcgagg
     5281 ctttgtgctg ctagaactca tcgcggatgg ggacgatgaa gacctcgctg tggtggagca
     5341 attgcgatcg gcgggcttcg gtgccaccgt gatccgcggg gaagggaaat gcggcggccg
     5401 gtgcatcgtc aaggtcgtat gcagccgcaa agacgtggat tctgcctcgc tcatcatcgg
     5461 aaaccggggc tttgtggtgc tctccgacgt gcgaggggtc tggagggggt acttccgggg
     5521 ggttaggaag tgatcttttc cactccagtg ctcctcgact cctttcttta tcctgtggac
     5581 gaaacggcac cgctaaaggg acgatagccg tcgccgggac ccaggaggca caaagacccc
     5641 ccgtccgatt ctctaggagg cgtcgacggt cgagccctcg gaactctccg ccgacctaag
     5701 atcggatttt ttcggatgac acccttgacg ggggctcggt ttccctatat aattcccctg
     5761 ttctcgggac gtggcgcagt ctggctagcg cacttgcatg gggtgcaagg ggtcgtaggt
     5821 tcgaatccta tcgtcccgac cagaggacca aggggggaat ccagtggatt ctcccctttt
     5881 tttcatgggg gtggtgcatc atgcctcggg ggattccctt ttctccccgt acggaaatag
     5941 gtcaaaactt tctcgtggac cggaacttgc cccgtattat cgtggaagac gccgggattt
     6001 gccccggaga gacggtgctt gagatcggtc cagggaaggg ggtcctgacc aaggcgctct
     6061 tggattcgcc ctgcggtcgt ctcgacgccg tggagctgga tcgccgcctg gcccctttcc
     6121 tggagcccta tcagaaaaac gagcctcagc gatttttctt gcactggggg gacgcggtgc
     6181 gtttcgacta ttcttcgttg ccccctccca caggggtggt ggccaatatc ccctatcaca
     6241 tcaccacgcc gttgatatgg aaaatcttgg aggatttggc cccccaaggt ctttcgcgtc
     6301 tggtcctcat gatccaaaaa gaggccgccc ttcggctcgc ggcgcccccg cgctccaagg
     6361 cccgctatcc tttgggagtc acgctggagc tcatgggagg ggtgcaaata ttgcgccccg
     6421 tccctcccga ggcctttcgc cccattcccc gggtcgattc cgccctggta cgcatcgacc
     6481 tgaaggccca tagggacctt cccgccatcg cctcatggag agagctgctt cgaagggcct
     6541 tcggagagcg aagaaagatg ttacgaaatt ccctggaagg ccttcctctg cccctggcgt
     6601 gcccctcctg ggaaacctgc ctagaggccc taggcctccc gacgacgtcc cggccggagg
     6661 agctggatgg agaggcctgg atcgagcttt ggcgctccct ggaaggcaca aaaagaccaa
     6721 gcggggcacc atcggcctga cgccgactct gccccgcttg gcgctttatc aaaggttcga
     6781 tgggtggaaa ggtcgcggcg ctacttcgcc ggggtggcct tcttggcctt cggctttttg
     6841 cactccgccg tggccgccac cgctgtcccg gcccccttct ttgtacgtcc ctttttgact
     6901 tccaccgctt ccttcagtcc cttgcccggg cggaaaacgg gcgcgttttt agcggggatc
     6961 tcgataacct tttcgggatc ctgggggttt cgtccctgtc gggccgcccg ctcacgcacc
     7021 tcgaaagtcc caaaccccac gagctgtact ttgtccccct tggcaagcgc cgcctgcaca
     7081 gcgtcaaaga ccgcgtccac aaactccgcc gattcctttt tgctcttatt tgtcgcctta
     7141 gccacttcgc ttaccagatc actcttggtc accgtgccac acctcccttt gagatcgggg
     7201 cctccgaccg ttccgtcgag gtctcccctc gtgtcattat tagcagcttt tcacagcgcg
     7261 gtcaaggggc tccatgggcg gatttgcgtt ctcccgtcct ccaataaagg cgcatgggag
     7321 caccatcgaa gagggcgacg ccccgcaatc gcttctccac gtagcgagaa aagcccttgg
     7381 tgactatgtc ggggtcgttg acgaaaaagg cgaagctcgg gggagcggtg cgcacctggg
     7441 aggcgtaata gatacgcaaa aactttcccg ccccgtcgct cggaaggcgc tcaaaggcca
     7501 tcaggtctcg aagaattcgg tttagctccc ccgtgggaat ccttcggcgc cagttttcgt
     7561 aaacctgcag gacgtgttga gggatttttt gcagtccccg tcccgtcttc gccgacacga
     7621 aggccgtggg agcatggtcc acgaaggcaa attcccgccg caggtatttt ctcatacggt
     7681 cccccagaga ttcctcccgg ggggccagat cccacttgtt gacgacgagg accaaccctc
     7741 tccctcgctc caaaacgtgc cctgccaaac gcttttcttg atctgtggcg ggctctcccc
     7801 cttctatgac gagaacggcc acggcacaac ggtcgatggc ctcatagctg cgcaacatgg
     7861 aatagtactc cacatcggaa gagatgcggg accgacgtcg caggcccgcc gtgtccacaa
     7921 aacgaaaggg agtgccgtca atttccacta gggaatccac cgcatcccga gtggtacccg
     7981 ggatggggct caccaaagat cgctcctccc cggccaaggc gttgaagatg cttgatttac
     8041 ccacgttagg gcgcccgatg atcgccaccc gaagctcgtc cgagaggtcc tccggcggga
     8101 gttcctcggg gggcaaaagg gccaccacta tttccatgag ctctccgatg tttctccgat
     8161 gctccgcgct taccgcgacg aggcagtcaa accccaggga ataggctccc tgggcctcgt
     8221 cttcgtgtct ggggtcgtcc gctttgttgg ccacaaggat caccggacat cgggccgatt
     8281 ttctcagaac ctgggcgatg tcttgatcta agggcgtggc cccctcccgg ccgtcgatga
     8341 cgaaaagcac cgcatcgctt tcttccagag aaaggcgcac ctggcgcacg atcccctcca
     8401 tgaggggttc gtcgctcccc gtgacgatac cccccgtgtc caccacgtaa aagcgacgat
     8461 cctgccagca ggcctccccg taaaggcgat cccgagttac cccaggaacg tcgtccacga
     8521 tggcgaggcg ccggccaatc aacctattga acagggacga cttccccacg ttgggacgcc
     8581 ccacaatggt cacgatggcc atgattgttc ctcctaattc cgcaggccat ggcaatggcc
     8641 cctattccct ctccaggacg atcctgggaa agggcccctt acgacggatg cgaaagaatg
     8701 gttccagggc ccggcgcgcc gcccccaggt gctccgggcg agcccagagc atgccgtctc
     8761 caaggccggg atcgagcact tctacccctt gggattccag cgccttccgc agcgcctctt
     8821 tgtcctctag tcccgcttcc agggagagca aggggcgata gggaggaagg ttgtagagcc
     8881 ttctttcctc catctcccgc gcccaaaagg ccccccatcc ccgaggaagg ctttcctgcc
     8941 acccccgtag gggagaccgg ctttgcacca ccacggtgcg atcttcgcac cgccctcccc
     9001 gccacagtga ttcccatatc atgcgaaagg ccacgaaccg ggcatcatag cgcggctggc
     9061 gagcttccgc gtccgcatcg atccacgcca cacatccgac cgagagatgg tcgcacagcg
     9121 ccagggctcc ccgagtcccc aagacgacgc ccccgctctt tcggagggct cggcgggctt
     9181 ccaaagttcc ccttttccct ccggcccaaa agatcaccgg aaaatcacct cgaaaaagat
     9241 ccgaggcggc ctcccaaagc gtctcaatcc ccggcctcaa cccctggagc aggattcctc
     9301 ggcacaccga acaggaggga tcttgggctt gaggggtgcc gcaaaagggg cacgcatagc
     9361 gcccccgctc ccatcgacag gttccaccgc aggaggggca acggagggga gccccgcact
     9421 ccgcgcaggc cacttcgctg gcatatccct tgcggtccaa aatccaaaga gctcccattc
     9481 ctcgctggac cgccctcttc gtctcccgaa aggtcgccgc gccgatgcgc aacggatctc
     9541 ggacccctgc cactgccacc gcatccgtgc ggcggaggtc cacgaaaaag agccgatccc
     9601 ccgggcgctc cgatcccctc cccccctcca tgcggaaaag cctcgccgag ggcattcgcc
     9661 cccccgccag gaagcgggat ctccaaaggc gggctctctg agaggccacc gtgcgggcgt
     9721 gcaaaaacgg gggatctacg aaacggtgcc cgccactggc ttcttcgtcc accaccacca
     9781 gccggaggtc tcttaagggg gcaaagaggg cccctcgccc tcccaccacc aacgaccttt
     9841 ctccggatcg gacggcttcc caggctcgct gaagggcccg cccccccgag gagggccaaa
     9901 ggatccctcg ccctctttcc ctgtctggca aaagatccca gaatcgggca gcgtcacttt
     9961 gttcaccaaa aagcgccagg atccccccgg atacgccccc gatctcttcc cgatagcgcc
    10021 cccaacggcg atcgtcccga gggtcgaaga caaaggcgtc ctccgaatcg ccggacgacg
    10081 ccctcaccgg aaggtcttcc tgaaaggccc cctggccccg caaaagaaac cccggggcga
    10141 tgcgtcccaa agccagggga gtgccgcaaa gataattaat tcctatccaa gaggccagac
    10201 gccccagctc ttctcccagg ggaggagctc catccagaac gtcttcaatc tctttcagct
    10261 ctccttgccc cggaggaggg gaagggccgt ccggcgccac gaatcccacc cgatggccac
    10321 gccctacggg gaccttcacc cgaacccccg gaggaagggg gcgatcaaag cggtacgtca
    10381 gggcatgcca ccaagggccg gggacgcaga cctccagggt gatctccatc agacttgaga
    10441 aaggcgttgg accaccgcgt cccaaatgcc gtcggccacc tcgtctttgg agcctgcgag
    10501 gacctggtct tccgcccaag ggctcagcag ctccactcgg ttggtctctc gacggaaagc
    10561 cccttcttcg ccggagacca ggttggcggc gatgaaatca aggttttttc ggcgcatctt
    10621 ctcctccgcg tgggcccgga ggtcctccgt ctcggcggca aatcccacga ggatctggtg
    10681 cgggcgcttg cgagatccca cctcggcggc gatgtccgga ttagccacca ggtcgatcgt
    10741 cctgagagcc tgcccctccc ttttgatctt ctgggaagag ggggacgccc ctcggtagtc
    10801 ccccacggcg gcggccttga cgatgacggc ggcgtcgtcc atttctgcca ggaccgcccg
    10861 gcgcatctcc tccgccgagg taaccgagat gtggcgcacg ccatggaggg gagagagggc
    10921 cacggggccg gagatcaggc acacctgggc cccccgatac caggcggtgc gagccagggc
    10981 aaagcccata cgcccgctgc tgggattgga gataaatcgc accggatcaa ggaactccca
    11041 cgtgggtcct gcggtgatca ccaccttgcg ccccactaaa tcagggagcg ggcggagcct
    11101 tcgccagatt tcttccagga tgacctccac ggaaggaagg cgtccccttc cctcgtagcc
    11161 gcaggctagc gctccctctt cgggctccac cacgccataa ccccacgccc gacactcctc
    11221 cacggaccgt ctcgtggtgg gatggtccca catgtgctcg ttcatggcgg gaaagagcac
    11281 caccggagct cgggtcgcca agatagcggc ccccagaagg gtttcggcct ctccccgggc
    11341 ggcccggcgt aaggtgttgg cggtggcggg ggccacgcaa agggtttcag ctcggcccgc
    11401 caaggtaata tgggggatct gcgccccccc tgcggggctc aagaagtcct tttggcgcca
    11461 aacccggcgt cccgataggg tacccagcac caaggggctg acgaaactct ccgccgcctc
    11521 ggtgaggata atgtccacct cacagcccgt gcgccggagt ccccggacga tttctggagc
    11581 cttataggcc gcaatacccc cggaaatgcc aagcagcacc cgacggttat tcttccacca
    11641 cgacatgttc ggggacccct ggcacggcct tgcgctccac cgcagggcat gagtggcgga
    11701 gtttcccgtc gaccaactcc tgaagggccg tcgatatgta cttttcggat gaatccccgg
    11761 cgacatacct ccccttttgt tcactcaaaa aacgcgcccg ctgagacaca atgacggtaa
    11821 ggacgtactt gctttctatg tcgaacttct cacataactc ttccaggtcg gaaaaaatca
    11881 cccttctcac cttcccttta ttccatcgac acacgcccgc accgcaggaa ctaggcaatc
    11941 cagggctttt tccaggtcgt cgttcaccac cacctggtca taaaacgacg cttgttccat
    12001 ctcgcgccgg gcattctcca tgcggaggac caaggtgtcc tctccttcgc tgcctcgatg
    12061 ccgcaaccgg tcttggaggg cctccgagga cgggggcatg ataaagatca acagcgcgtc
    12121 ggggaaaagg gctttaaccc gtcgggcccc ctggacgtcg atctcaagta ccacacactt
    12181 tccggcctcc aatcctcgct ctacgtccgc ccaccgggtg ccgtagtgat ggccgtggac
    12241 ctcggcccac tcgagaaaag cctcttggtc gcgccacagg gaaaagtctt cctccgacac
    12301 gaaccaatag tccaccccgt cctgctctcc ggcccgggga ggtcgggtgg tgcaggagat
    12361 ggaaaaaact aattccggga tcttctcgaa aaggcgcttg cggagcgtcc cctttcccgc
    12421 cccgctaggg cccgatagta cgatgactcg tcccctattc ttcgccgtcg ccatcgcctt
    12481 cttcttcctc gacgaaacgg tgggccaggg tctctggctg aagggccgaa aggatcacgt
    12541 ggttgctgtc catgatgagc accgatcggg tcttccttcc ttgggtggcg tccaccaaaa
    12601 gccctgcccc ccgggcctct tctttgaggc gcttgatggg ggccgaagaa gggctgatga
    12661 tgcccactac tcggtcgctc accaccatgt tgccgaatcc tacgtgcagc agccggtact
    12721 gcaaggggca tcactccaca ttctggattt gctcgcgcat ccgctccaga tcggccttag
    12781 cttccactac cagccagcgg atccggccat ctgaaacctt ggatcccatg gtgtttattt
    12841 ctcgattcat ctcctggacg aggaagtcga gctttctccc cactcctcca tcttcagcca
    12901 ttacagaccg aaactgagcc atgtggcttt cggagcgaga gagctcttcg gccacgtccc
    12961 atcgatccgc cagcacagcg gcctcttggg cgaatcgggc ctcatccagg cgccacccgc
    13021 cctgatctag cagggcctgg aggcgggtgc ccatgctccg cagggcttcg tcccgagcac
    13081 cgggccaaag agatcggatc tcgtccaggc ggccacggta agaggccaaa tggcccaaga
    13141 ggtcttcttc catatgtttc ccttcgtttc gtcgcatggc ctgccacgac tccatcgccg
    13201 cctttagggt ctcctggagg tcaaattcca attgttcacg aaccttttcc tgaggttccc
    13261 cggtctctaa gacccccgga aggcccagat agctctccaa ctccccgctc cggggaaatc
    13321 tcaacttctc tcccacggcc gccacgctcc gcacgtactt ttccagcaca tcttcccgca
    13381 cggaagcagc ccgcaaggtg ggagcccaag ataccgccac ccggacttcc gccttgcctc
    13441 gattgaagga ttggcgaatc tgtcggtcca aggggatctc ccatcccccc aactcccggg
    13501 gaaggcgtag ggaaacctcc tggaaacgat gattcacgct ggagatctcc accgaaagat
    13561 ttccccaggt cgcacttttc tggcaccggc caaaaccggt catgctaatc agcatgtcga
    13621 acccccatcc tctccgaaag gcccgagact tctttccccg cgcacgcccc cgccaaagag
    13681 cacgtcatcc acggcccggc aaagggattt caaatcggtc cctcgaaggg cactcacggc
    13741 ccgaacggtc tccccttcct cgctccaccg cctgagaagg ctctctcgcg cctccggagg
    13801 ggccaggtct tctttgttaa gaactataat tcgaggcaat gctccggccc ccaggtcttt
    13861 cagcgtggat tgcaccactt ccagatcgcc ctggatgtcc tgggcggtta gatctaaaac
    13921 gatgaggagg agatccgact cccgcacttc ttccagggtc gcccgaaaag ccgccaccag
    13981 gtcgtggggc aacttgcgca cgaatcccac cgtgtccgcc accatcaccc gctctcccga
    14041 gggcaaaaag acggggcgaa gggccgtgtc caacgtggca aagagtttat ccgccacaaa
    14101 aatcgaaggg tccttggaga ggctccgcag gagcgtggat ttgccgctat tggtataccc
    14161 cacgagggcc acggtgaaaa acccttggcg gcgccttcgc tccctttggc cctgacgccg
    14221 ctggcgcacc aaatccaagc gcttcatgat gtctcgctct cgtcggtgaa attttctccg
    14281 atgccgctca aattccgtct ccccaggccc ccgggtgccg atgccccctc cggtacggga
    14341 catctgctgt cccaacccct tcagcgaggg cagttccatg cgacatcggg cgaggtcaat
    14401 ttggagcttg gcctccgccg aatgagcccg ggcatcaaaa agacgcatga tgacccgcgc
    14461 ccggtcccat acttcaagtt ttgtcagctg ggataggtgg aaaagctgac tcggagagag
    14521 gacgtcatcg cagacgacca ttttagcccc cacggcctgg gccgccgccg ccgcttccag
    14581 tgccttcccc tgaccgatgt agtaggccgg atcgggggca tcccggcgtt ggaccaccga
    14641 agctcccacc gtcactccca tgttgccgag aagaagagaa agctctccct gaaggagagc
    14701 tacttcctcg tcggtctggt ttttctgctg aacaaacagc gtcaccgccc gggagctctt
    14761 caaggactcc ccgccccctt tgcccccatg gatcgaaggg ccccctcgac cctttccact
    14821 aagaggcggc gccgggtctt gtcaggcatc tcagagggga tcgatgcggg gtcaatcgga
    14881 ggaccgtaac agattgaaac gggtttccat cgccactttc cccccggggg aagggccgca
    14941 aaggtgccgg agatcacggc gggcaccaca ggaactccca ttttgaggga aagaagggca
    15001 aatcctccct ccaggggctg aagaaaaccg tcccgcgatc ggtgtccctc gggaaaaagg
    15061 agcaccgatt gcccttcttc caggaggtcc agcaacaatt ttaacacggc cccggcgcgc
    15121 tggctgtcct ctcgctccac ggcgatagcc cccaagttgc ggatcaaaaa gccaaaaagg
    15181 ggggcacgaa agagctctgt tttggccaag tagcgcaacg tccagggaaa agagacccct
    15241 accaccatgg ggtccaggtt actgcagtgg ttcgccacga cgatgaccct tcggggaagg
    15301 cctagggagt cctgtcctcg cacgcggaaa cggtgaaagc aaaggagaaa ggcccgcaac
    15361 accccgcagg taaggacata aaaaagacga tagggacccc tatgcactgg aacaatccgc
    15421 ctttcggaaa agggtcacga tctcttccac tacttcatcc accgttttac catccgtcgc
    15481 cacgaggacc gcgttctcgg cggggcgtag gggggctact tctcgagagg cgtcttggcc
    15541 gtcgcgatga cggatcccct cccgtacgac ttccatgctc acctcttcgc ctcggagacg
    15601 tcgttcttcg aaacgccgcc gggcccgctc ttcgtccgaa gcggtaagaa agattttaag
    15661 gctagcccga ggaaagacca ccgtgcccat gtctcgcccc tccgccacca gtcccccgga
    15721 gcgggcctgc tggcgctgga ggtccaagag ccgatctcgg accgggggca gggccgcata
    15781 ggacgagacc acggagtcca cccgggaaga tcgcaaaaac tccgtgacat cttccgatcc
    15841 cacccacacc cggccctctc tgagatccac atggagcgcc tccagggccc gaaggagctc
    15901 ggggccttcg gcgggcaaaa caccccctct gtccaaaacg aacgccaacg cccgataaag
    15961 ggcccccgtg tccaggtaag acaggcctaa ccgggccgct aggcgctttg ccaccgtact
    16021 ctttcccgct cccgcaggac catccaacgt caccaccggg gtcatggaca tcagtccccc
    16081 aggcggaact cttcgtccac ttgagagatg aaatccacca cctcggacat gagtccctct
    16141 atgctttcta ggcgcaatcg ctccatggcc gcctctgaca gggtgtattg caacccgtcc
    16201 gccccaattc ctagccccat catgaggcac acggcgttgg ccacgtgaac cacgtccacc
    16261 atacggcgaa tggtttgggg cacgtcctgg cattcttcgg ggttgtgatg aaagcgggcg
    16321 gcgtactggt aggtctccgg gaggttccac tgctccagca tcagccctcc cacctgggcg
    16381 tggttgaagc ccaggacttg cgtttctgcg tccatgaagg gcatgtattc gtcttccacc
    16441 aaacgcacga tgatgccgta gccgaagcgg acgaagtcgt tcaacacaat ctttcccagg
    16501 tcgtgcaaca tccctgccac gtaggcttct tcctcttcgc ctttgacctt cttggcgatg
    16561 tgccgggcca cgaaagccac actcagggag tgcttccaga gctgccctcg atccaaggcg
    16621 taccccgtaa aagctccgtc catgaattga tacaccgacg ccgaaaggac aatgcttttg
    16681 acggttttga aacccaaaag cgcaatagcc tccgtcacgc tcactatccg tcgaggaagc
    16741 ccatagtacg cagaattagc cagccgaaga atccggatca ccaacccttc gtcccgggag
    16801 aggcgattgg aaacatcggc tgcgctgctt ttggggtcgt caagtttttt tagcgtctcc
    16861 acgacgaact ggggcaacga gggaatttct ttgacgcgct tcaggaccct gcgctgaatc
    16921 agatctcggg tgcgttcgtc cagtccctgc atcctgcatc ctcccgctcc atccgacatc
    16981 tccgttgggg taagctcgcg ctcataccaa acccccagcc ctaatcatac accataactc
    17041 ctcccgggaa aacgtcatcc atcctcctgg ggggagtttt tcccatttta gcgctccgat
    17101 ggccacgcgg atcagccggg tgacctcata ccccaaagcc cgggccatca tccggacttc
    17161 gcgcttcatc ccttcccgaa gggtcacctc cacccccccc gagaatcggt ccaccccttc
    17221 gggaaccacc caacgcccct ccagatcgac gccccttcgc caccgcccca gatcttcggc
    17281 atccatgggg cgggagagct ccgcgcggta ctttttcgtc accccgaacc tcggatgggc
    17341 caccgattgg gcaaaatcgc cgtcgttcgt caagatgagg agtccttcgc tgtctttgtc
    17401 gagacgacct acgggaaaga gcccccgatc ctgaaatttt ggggggagaa cggtcagaac
    17461 cgtagggtaa aagcggtccc aaacggcgca caccaccccc cggggtttat aacagaccac
    17521 gtaacaaagc tcccggggca tcacgacccg cccgtccacg cgcacacgac ctccgggccc
    17581 caccctctct ccggggctta gggcccgctt tccgtccacc tccacccgtc cttggtgcac
    17641 cagatcttcc acgtgacgcc gggatcccaa ggcacaggag gccaggtatt tgttcagccg
    17701 caccgacccg gcggattcca tcacgccatg ccctcttctg aaaaggcctc cggcagggcc
    17761 tcacgcaact cttccaaggc tggaaggtcg gcaatggcct ggaggccaaa gagctccaaa
    17821 aagcgatccg tggtgcggaa aaggagaggg gtcccgctgc ctcggcgacg gcccgccact
    17881 cgcaccaggc cgtggcgaag gagggtttcc atgacccggt cgcatcgcac ccctcggatc
    17941 tcttccactt cgccccgggt gacgggctga cgataggcca ccaccgccag cgtctccaga
    18001 gatgccttgc taaggcggat cttctcgtcc aggtgatccg cccggaaggc gcgcaccgcc
    18061 tcttcttcct ctggggcggt gaccatctgc caccctcccg ccacctcgat aagagtcacc
    18121 ccgtgccccg cctcgtagtg ctcccgcaac tgggaaagga tgcgtcccac ttcccgggag
    18181 gaaatcccca ggaccgccgc caatgacgcc gaaggaacgg gctgggtcgc cacgaaaagc
    18241 agggcctcca aggtgcggat cgacggattc tggaggggcg cttcgccccg caccccgttc
    18301 atgggacact cccctccacg gcaggatttg cgcaccgggt cacgcgcaag gagccgaaac
    18361 aatcggactg taccagcttc acttcgttcc tccgggagag ctccagcaaa gccaacagaa
    18421 ccaagaccag cgccgaccgg cttctacgct cttctagaag gccatccagc tccacgggaa
    18481 cgtggggcgt catcagggac ctcaagcggt ccatgcagga ctctacttgg gattcctcgg
    18541 ggaccatgga ggggacgccc tcctcgtccc agaaatcttc ttcgcaaatc cctaccgagg
    18601 gccgtcgctt ttgccgtatc aggggccacc agaggcatgc cagggcatag acatccccca
    18661 ggtcgaagat cttctccccc tcttcggccc gccgccccac gtaggccgcc ctgcggtgaa
    18721 atcgctcttc catcatgcgc gccgccgccc gaaagggacg ataccgagcc agggcttcct
    18781 ccacgtcctc ctcggtgcca ccgaccgtcc ccgttggatc ttcctcgggg gcgcccccct
    18841 cctcttcccg aaagatcccg ggcatgaggg cctgaagttt acccagcaag agccgagccg
    18901 ccagggcgaa gaattcggcc atctctcgaa tggtcatcac gtccccccga gcgaggtagg
    18961 cgccgtaaag gcgcaccacg tcgg
//
LOCUS       DAOOER010000035        18817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_3393_length_18817_cov_9.064066, whole genome shotgun sequence.
ACCESSION   DAOOER010000035 DAOOER010000000
VERSION     DAOOER010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 18817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18817
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3393_length_18817_cov_9.064066"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            242..2389
                     /locus_tag="PL162_06040"
     CDS             242..2389
                     /locus_tag="PL162_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HQF91617.1"
                     /translation="MRTEHAALVASSLHLRQEQVEAVAALFDEGCTLPFIARYRKERT
                     GSLDEVDLAAIRDRMAALAELDRRRNAVRASLEERGLLLPELGRALDAAKSLTEVEDL
                     YLPFRPKRRTRASVARERGLEPLADLLWAQKGILPLEAARPFVDAERGVPTEDDALDG
                     AGDIMAERVSESLEARRALRRLFVEKGTLSSRMMPGKEEAGGTYRDYFDWNEPLARIP
                     SHRVLAIFRGEAEGALRVHIAPPEPEALEILEKIFVHGRGADAEEVRDAVHDAWKRLL
                     QPSLETEARQAMKKRADDEAIHIFAENVRDVLMAPPLGEKRILAVDPGWRTGCKAVCL
                     DAQGNLLHRETFFLLSAHQREEARSQVDRLVAAHDLEAVGVGNGTGGRETEAFLREVF
                     ADRGIPVVTVNESGASVYSASEVAREEFPDLDLTYRGAVSIGRRLQDPLAELVKIDPK
                     SIGVGQYQHDVDQKALRQALDDTVERCVASVGVDLNTASQQLLERVPGVGPKLATQIL
                     AFRRKKGPFRSRSSLRRVPRLGPKAFEQAAGFLRIRGGEQPLDASAVHPEHYDLVGRM
                     ASDLGCTVEDLLRHEALRQRIDPSAYVQGTVGLPTLKDILEELAKPGRDPRSSFALPA
                     FSDQVHRVEDLAPGMVLPGVVTNVTAFGAFVDVGVHQDGLVHVSRLGKRGGSPFDVVR
                     VGQWVSVAVLEVDLPRRRIALSMRPEDLEGPPE"
     gene            2475..3014
                     /locus_tag="PL162_06045"
     CDS             2475..3014
                     /locus_tag="PL162_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91618.1"
                     /translation="MGTSLTEEILARDPGRILANQRLFAEILGQSARERRRLRHAGGA
                     REFPEGGRHGEGRPLVGEASWVLPPSHPLTPPPRPPEKISPFDPLEPLADQKRNEERL
                     EGISRARLFNKEDVLAWVRKNFDVISEWRRIPGTDIEQITLRDRSTGKVLLQLPLDEL
                     IPKILEGLRLRGAIFSRLT"
     gene            complement(3098..3871)
                     /locus_tag="PL162_06050"
     CDS             complement(3098..3871)
                     /locus_tag="PL162_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HQF91619.1"
                     /translation="MRLLFVGDVVGRPGREALARALPELRQQEGPFDFVLANGENAAA
                     GFGLTEKVMNDLFSLGVACLTGGNHIWDKKEFIPVLGGESRVLRPANYPPGCPGHGVA
                     HLSNGRSRLAVVSLQGRAFMPPVDCPFRAADAIIEALEERAVLVDFHAEATSEKWALF
                     RHLDGRVSAVVGTHTHVATADEEVLPGGTAVLTDVGMTGGHGGVIGMTTETTLCRFLD
                     ALPSKFAVCDTDLRLRGAVIDIDDETGRALAIRRVERRF"
     gene            complement(3871..5400)
                     /gene="rny"
                     /locus_tag="PL162_06055"
     CDS             complement(3871..5400)
                     /gene="rny"
                     /locus_tag="PL162_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HQF91620.1"
                     /translation="MEVLFFVAGLAAGIGGGFLLRKTTEERAMSDARARSEALLRDAT
                     AEGEQRKRELLTEAREENLRLRQEIDKEVKDRRNEMQRAERRLEQKEENLDRKLDALG
                     RREEELKAGQEALKRRMEDVDSLYTEQTRKLEEVAAMTREEAQQILLAAVEEEANHRI
                     GVRLRELEEKAKREADRKAQEIIATAIQRTCVEHTSEAAVSVVPLPSDEMKGRIIGRE
                     GRNIRAFETLTGVDLIVDDTPEAVTISSFDPVRREVSRRALERLITDGRIHPARIEEL
                     VEKCYQEVDQEMIDRGENALLEAGVKNMHPELAKLVGQLQFRTSYGQNTLMHSLEVAH
                     VAGIMAAELGVDEALARRAGLLHDIGKAVDHQVEGPHALIGADLARRYGEAPDVVNAI
                     ASHHEDEEPQTVHAVLVAAADAVSASRPGARRESLEAYIKRLEKLESVANSFHGVTKS
                     FAIQAGREVRVMVAPHQTDDASIAKMAFDIARKIEEEMKYPGQIRVTIVRESRAVEYA
                     K"
     gene            complement(5715..6014)
                     /locus_tag="PL162_06060"
     CDS             complement(5715..6014)
                     /locus_tag="PL162_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQF91621.1"
                     /translation="MKKKESFTKGDLVHEVAESTGQKIKDVTVAVDAVLDAVMGAVAG
                     GRRVSLVGFGVFEARVRAPRQGRNPQAPDQVVEIPARTVPAFKPGKAFKDRVADS"
     gene            complement(6051..6743)
                     /locus_tag="PL162_06065"
     CDS             complement(6051..6743)
                     /locus_tag="PL162_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91622.1"
                     /translation="MQRRRFFDGKDRPVTVGDILRAMESSSWPGFVTRAAEQVLQWMM
                     GELAAEEAFYEEENPAEGVDPRMMGDTENLFLQLSWLEESVDLLPTQNGGPGRGWPEA
                     LVLAIGPIDIDEGLRIAVDHAALFARGKCHRVWVFCDNWIVGDALRYLPHVRMLADQG
                     VELRFVLLTPWGWTEIPLGKDPDKKGPLPWRGSSDEGLGGASPRFSKEGRNRNAPHGA
                     GDDPGGASGKPH"
     gene            6957..7514
                     /locus_tag="PL162_06070"
     CDS             6957..7514
                     /locus_tag="PL162_06070"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HQF91623.1"
                     /translation="MKPFVLDARRSLVLLIDMQEKLMPAIAHREEIEKTGALLLRCAD
                     ALGVPVLVSEHYSKGLGKTLPSLVQVLPEKSPVVEKISFSCWGEPAIVRTLEETARRQ
                     VILLGVETHICVLSTALELLAEGYDVVLASDATGSRREVHRDEAFAALRSAGGTVLPT
                     ESVVYQLLGQAGTELFKRVLPFFKA"
     gene            7519..8352
                     /locus_tag="PL162_06075"
     CDS             7519..8352
                     /locus_tag="PL162_06075"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQF91624.1"
                     /translation="MPSAEPLSGYPKNFIRFLGTAGARFTVLHQMRASGGLWVRLGGA
                     SWVVDPGPGSLVRICEARPPLDPESLDALVLTHRHIDHSTDLNVLAEALTGGGRRRHG
                     CVVLPEDALSGPEPVFFRHVRDRVDRVEIWGPERGVLLPGGTELTPVPLEHHGVLCFG
                     FVARSPGLPTWGVVSDTRPLPHLGKLLSSCDMIVAHTTLRHAVDTIDHLCLEDARTMA
                     EVASPRLMILTHLGRGILQEDPRRLAEEMSSDRTRFIAAWDGMVLNLDTLEPESPYSG
                     T"
     gene            8537..9712
                     /locus_tag="PL162_06080"
     CDS             8537..9712
                     /locus_tag="PL162_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQF91625.1"
                     /translation="MPYIARRTREHPPGVPFRLINLLRELRHQGEDLIDLGWGGPHRL
                     PAPHVLASVVDGCRAEDAFSSPHIIPSLPEAIGEWYRSRFGVSLDRNSEILPVWGNLE
                     GLLSLPLAFLDTGDGILLPNPGNPQYVFLSSTVDAQVTFYPLSPARSFLPSFEALKPL
                     VTPRVRLLVLQLPSDPTATVGSRDLLAQAVDFCRRHDLVLVHDRRLGEITDDGVSSPS
                     VFEVPGAREVAVEYGSFTYSFHMAGARLGYFVGGSPLIAAMARTRGPFPLGPFLPIQR
                     AAIQALTHSDRDAAIEGVRVLYRDRRNALLQALASVGWAIPPSPCPLYVWAPVPGGDD
                     DRFVERCLIEAGVVFTPGRTFGDAGRGYVRMDLSKEELPRLMEAVDRLQPLLVPLLS"
     gene            9797..11050
                     /locus_tag="PL162_06085"
     CDS             9797..11050
                     /locus_tag="PL162_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HQF91626.1"
                     /translation="MLVYDRIRSLELHRRARGKVKIFPTISVNNEEDAFLASYPGSVA
                     PGMAIVENKDMVYEYTGKGNRLAVVSDGSAICGMGNVGPLAGMPMIEGKCLLFKLFGD
                     VNAIPLCVSSENPDEFIHMVSMIAPNFGGINLEDISSPNTFTILRKLERMLDMPVFCD
                     DQDGTAMLVVGALINAMKVVGKKLEDIRIVFCGAGAGGIAAAQLLLHAGARNLVLLNS
                     KGILGPQNPSMNYVQEELAARTNPEGLTGGLEQVVRGADVLVGISSSGLFSGDLIRSM
                     APRSVVFALALPTPEISYEEAVAAGAAVVGTGLSRTGGKNQLTHMLAFPGLLRGAMDV
                     RSKDIPQELLLAGAQAMASVVDPRRLSPQNVVPDLFCDETTPRVAEAVAQRAVELNLA
                     QNPLPPGEVYNSAWQRIFGNRQGGI"
     gene            complement(11139..11678)
                     /locus_tag="PL162_06090"
     CDS             complement(11139..11678)
                     /locus_tag="PL162_06090"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HQF91627.1"
                     /translation="MKDDVVTGHQRAELPGLVGVWRLAWEDEGLVELAPLSHGKLIPS
                     GEAVLAEGQGVLPSWLWDGWIRFWRGEDPAVPLVGRVPPFFGRVYAVVRRIPVGGART
                     YRQVAEEVGVPEGARAVGQAMRKNPWALFVPCHRVVRTDGGLAGYGGPTGTPLKRALL
                     LMEGVTALPGGVSLKGGRP"
     gene            complement(11686..12261)
                     /locus_tag="PL162_06095"
     CDS             complement(11686..12261)
                     /locus_tag="PL162_06095"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HQF91628.1"
                     /translation="MAKVDLPRWGERIFPWRGGIWTILFLLILGYARPSLRSCFLGLI
                     PLVGGQLLRFWAAGTIGRYRGEEVGAVQLVTWGPYAFVRNPLYLGNAAIGLGWAIMAN
                     SPGVLGVFLLAFLAIYGGAIIPYEETFLEKKFGPAFRAYRDRTPMFFPRPPFSKKWRG
                     PFDRAVLWRSERHSLWVTLGGSAALISRLWW"
     gene            complement(12248..13336)
                     /locus_tag="PL162_06100"
     CDS             complement(12248..13336)
                     /locus_tag="PL162_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HQF91629.1"
                     /translation="MTFRRGVALFLIISIAVVVAILSFSADRHTMKILMNARKEGIVL
                     ALMMVGAIWFCDATRFRLFSRAAGERLPLRLAFVLTWINYFGCAITPMQGGGGPFQMY
                     VLYRHGVSLGKGVAITLVRTLVTLFLLGLTLPVTLLVEPQILRGQGFIQGMFIWILVF
                     VVGAWILITLSLVRPRLIKRWCGVLTFWLNRAGVVHRRSILRVVRRIHREIDEYNNNI
                     RVFCTTGKWYFLGAILVSCLYLFCIFSVLPCLIWSLGQPVHFVECVLVQAMFMFILYF
                     VPTPGASGVAEGGAMVLYKLLVPWNLAGVVAIGWRFFTEYTGIVMGAFVAMRYIGWDL
                     ASRLMDQEKAEEKAEEKVGCPGGDDGKG"
     gene            complement(13401..13955)
                     /locus_tag="PL162_06105"
     CDS             complement(13401..13955)
                     /locus_tag="PL162_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91630.1"
                     /translation="MAGTKEELWEALNGHLASLGRICRALEVDGEAMEVEAFLQVTGG
                     QELRVTSCPVRELVRESLGQAIRYLPELHKGAEKVADLLEEENLPEAFPALSQLSEGT
                     TWVLQVLGRSQVLLALSDDEIGDGELSAVRRRLLAVLRDALSSMEEKKFFELAFRLRE
                     ELAPTLVRLGGYLVALNNTAESVQ"
     gene            complement(14302..15657)
                     /locus_tag="PL162_06110"
     CDS             complement(14302..15657)
                     /locus_tag="PL162_06110"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQF91631.1"
                     /translation="MHDRKKAWVAVLLAWVGIGAFAGISGASEVPLTADGAVTLALEH
                     NPMVLVLQEKISQDEAQVREARAAGGPKLDALLAYTRVNEAPRSVALDPVTGNPVGVV
                     PLGFESAYKVALQLSQVIFSGGSIEAKIQSARWKVRATEAERSRTLQGVANGARRAYY
                     SVKRAGDKLAVAEEARSLAQEHLKQVESFFRNGVVAKNEVLRVEVDVAGAELAVIRAQ
                     NAVDVAWTALERAVGAPLRSSWTLAEGEEGAPVPHEGGDPESRALASRPEIRMLESSR
                     QAALFLAEAAAGAAKPQVMIQGEVGRQGDSFFPEEDEWKITLAAQWRLYDSGEVAARV
                     DQARGVARELLARIGDLESQVRLEVSTARFNLTSAQASVGVAHNQVASAEEDYRMALK
                     RYEAQVGTNLDVLDARLALNQARTALVDAIYDAWSADADLRYAVGEEDFVPASRGAVK
                     P"
     gene            complement(15825..17072)
                     /locus_tag="PL162_06115"
     CDS             complement(15825..17072)
                     /locus_tag="PL162_06115"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HQF91632.1"
                     /translation="MRMQVSEAREGMVLAQDLLAPNGRRILPAGAVIKASYLQTMKVW
                     GVTELVVEGGGEEEGTDRLSPEEEERRQRAERMVSRFFAATNRSHEAIEELRRLCTLR
                     VAEQLAKNSGAMGEEPPGQPEGMDLDAPGRRPALSDLVQREVQLCSFPDIYFRIKEVL
                     DSPRSSASHVAEVVSKDTSLSAKLLRVVNSAFYGFPSRIDSIFRAVAIIGSNELSTLA
                     LGISAINLFKDIPPNCVNMRSFWEHSVACGVYARVLAFSKKSSHEERFFVGGLLHDIG
                     RLVLFRRYPKYMTRALYLSKERSCQLFEAEGEVFDFSHAAVGGLLLAEWKLPPVVADM
                     VRYHHNPTKAPTLFDASVLHLADIIAGAMRRGSSGSFLVSALDDEAWKALDLPVSALE
                     SAIAQGERQIREIIHAFFGDEKP"
     gene            complement(17212..>18817)
                     /locus_tag="PL162_06120"
     CDS             complement(17212..>18817)
                     /locus_tag="PL162_06120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQF91633.1"
                     /translation="NRDLEAATEAANRLAMVAEKANRAKSDFLANMSHEIRTPMNGVI
                     GMAELLRDTDLTDDQRDQVDVILGSARALLHILNDVLDFSKIEAGKLEIFPAPFDLRE
                     VVESVGLLFSVKAAEKGLELLVRYAPEAPRYVVGDGGRLRQILVNLCGNAVKFSERGW
                     IEVAVACVESDEHRASFVIEVTDTGIGMDEETQGRLFQKFYQADGSSSRARGGTGLGL
                     AIARMLLELMGGSISVSSRPGVGSTFRVALSLPRDEREGEEERRILGKGAFVGRRALL
                     ALPWEGWRKALAEQLSYWGVECVEGRSPQLVLQELRSRGPSYHVIFLDSVWEDIRDMD
                     MPGMALPSVVLVASPGDQRENGGAGAEERLTKPLRGHALWNVMERLGRSQPFTGAADP
                     APDRDSPRRIELPREEGEGTFRILLVEDNKVNRTVAQGILRKFGFQADEAVNGQEAVE
                     RLRFVDYDLVLMDCSMPVMDGFQATRVIREEEGLRRHVPIVAMTAHAMSGDRDMCLEA
                     GMDDYLSKPVRPQELKDMLDRHLRKG"
BASE COUNT         3388 a         6481 c         5558 g         3390 t
ORIGIN      
        1 agcgaaaaat tcccgaccga ggcatgaggg gactttctga aagatctacc gggttccgcc
       61 ccctttcttt cggatttttc cgccgccagg gctctccaca aggcccgggg ggcgactccg
      121 cagggtgttc atcttaataa aaccttgcga tgccccgaca attgctataa tggcccgaag
      181 ggagcctgcg aacctccctc accgtgattt tttgtttttt aacaaaggag gatggacccc
      241 tatgcgcacc gaacatgccg ccctggtggc ttcgagcctc cacctccggc aggagcaggt
      301 ggaggcggtg gccgcccttt tcgacgaggg atgcaccctg ccttttatcg cccgctaccg
      361 gaaggagcgc accggatccc tggacgaggt ggacctcgcc gccattcggg accggatggc
      421 cgccctggcg gagctggacc gccgccgcaa cgccgtgcgg gcctctctgg aggaacgggg
      481 gctcctcctc ccggagctcg gccgggccct ggacgccgcc aaatccctca cggaggtgga
      541 agacctctac ctccccttcc gtcccaagcg ccgcacccgg gcctctgtgg cccgggaacg
      601 gggtctggag cccctggccg acctcctctg ggcccaaaaa gggatcttgc ccctggaagc
      661 ggcccgcccc ttcgtcgatg ccgaaagggg cgtccccacc gaggacgacg ctctggatgg
      721 ggcgggagac atcatggccg agcgcgtgag tgagtccctg gaggcccgcc gggccctccg
      781 tcgcctcttc gtcgagaaag ggaccctctc ttcccggatg atgccgggca aggaggaggc
      841 gggggggaca taccgggact acttcgactg gaatgagcct ctggcccgaa taccatcgca
      901 ccgcgtcctg gccatcttcc ggggagaggc cgagggggcc cttcgggttc acatcgcccc
      961 cccggagccc gaggccctag aaatcctgga aaagatcttc gtccacggcc gaggggccga
     1021 cgcggaggag gtccgcgatg cggtccacga tgcctggaag cgccttcttc agccctcgct
     1081 ggagacggag gcccgccagg cgatgaaaaa gcgggccgat gacgaagcca tccacatctt
     1141 cgcggaaaac gtccgggacg tcctcatggc gccccccctg ggggaaaaaa gaatcctcgc
     1201 cgtggatccg gggtggcgca ccggatgcaa ggccgtgtgc ctggacgccc agggaaacct
     1261 tttgcaccgg gagacgttct ttctcctttc cgcccaccaa cgggaagagg cccgtagcca
     1321 ggtggaccgc ctcgtggcgg cccacgacct tgaggcggtg ggggtgggca atggcacggg
     1381 gggacgggaa acggaggcct ttctccggga ggtcttcgcg gatcggggca ttccggtggt
     1441 gacggtgaac gaaagcggcg cctcggtgta ctccgcctcg gaggtggccc gggaggagtt
     1501 ccccgacctc gatctgacct accgaggggc ggtgtccatc ggccgccgcc tccaggaccc
     1561 cctggcggaa ttggttaaaa tcgatcccaa gtccatcggc gtggggcagt accaacacga
     1621 tgtggaccaa aaggccctgc gccaggccct ggatgacacg gtggaacggt gcgtggcgag
     1681 cgtgggggtg gacctgaaca ccgccagcca gcagcttttg gagcgggtac ccggggtggg
     1741 gcccaagttg gcgacccaga ttttggcctt tcgccggaag aaggggccct ttcgatcccg
     1801 gagctccctc cgccgggtgc cccgcctggg gcccaaggcc ttcgagcagg cagcgggctt
     1861 tttgcgcatc cggggagggg aacaacccct ggacgccagc gccgtccacc ccgagcacta
     1921 tgacctggtg ggacgcatgg cctccgacct aggttgcacc gtggaagatc tcctgcggca
     1981 cgaggccctg cgccaacgca tcgacccctc ggcctacgtc cagggcaccg tgggcctacc
     2041 caccctcaaa gacatcctgg aagagctggc caagcccggg cgggatcctc gatcctcctt
     2101 cgccctcccc gccttctccg accaggtgca ccgggtggag gacctggccc cgggcatggt
     2161 gctgcccggg gtggtgacga acgtcacggc cttcggggcc ttcgtggacg tgggggtgca
     2221 ccaggatggc ctggtccacg tgagccgcct gggaaaacga ggaggatcgc ccttcgacgt
     2281 ggtacgcgtg ggacagtggg tctccgtggc cgtcctcgag gtggacctgc cccggcgccg
     2341 catcgccctt tccatgcggc cggaggatct ggaaggtccc ccggagtgag tccaccccgt
     2401 ggcatttttt gcgccactgc gctataatag caccggagtg ttttcgataa acttcgacaa
     2461 aggggggtga tgtcatgggc acttctctga cggaagagat cctggcacga gaccccggga
     2521 ggatcttggc taaccaacgc cttttcgcgg aaatcctggg acagtcggcc cgggaacgcc
     2581 gtcgtcttcg acacgccgga ggggccaggg agttccccga gggggggcgc cacggcgagg
     2641 ggcgccccct ggtgggcgag gcttcctggg tcttgccccc ttctcacccc ctgacgcccc
     2701 cacctcgccc tccggagaaa atctccccct tcgatcctct ggagcccctg gcggatcaga
     2761 agcgcaacga ggaacggctg gaagggattt cccgggcccg cctcttcaac aaagaggacg
     2821 tgctggcttg ggtccgaaag aacttcgacg tgatctcgga atggcgccgg attcccggga
     2881 cggacatcga gcagatcacc ctgcgggacc gaagcacggg gaaagtcctt ctccagctgc
     2941 ccctggacga gctgatcccc aagatcctcg aagggctacg cctccgggga gcgatctttt
     3001 cccgcctcac ctaggggcgg agcaccacga aaaagcggcc cccttcggga aggttttccc
     3061 gaagggggcc gctggctgag gtccaaaaag gcccgggcta gaatcgtctt tccacgcgcc
     3121 ggatggccag ggcccgcccc gtctcgtcgt cgatgtcgat caccgcgccc cgaaggcgta
     3181 ggtcggtatc acacacggcg aacttcgagg gcagggcgtc caggaaacgg catagggtgg
     3241 tctccgtggt catgccgatc actcctccat gaccgccggt catgcccacg tcggtgagga
     3301 ccgccgtccc cccggggagc acttcttcgt ccgctgtggc cacgtgggtg tgggtaccca
     3361 ccacggcgga cacccggccg tcgaggtgcc ggaagagggc ccacttctcc gaggtggcct
     3421 cggcgtggaa atccaccagc accgcccgct cctccagagc ttcgatgatg gcgtccgccg
     3481 cacgaaaggg gcaatccacc gggggcatga aggcccgtcc ctggaggctc accaccgcta
     3541 ggcgagacct gccgttactc aaatgggcca ccccgtgccc cgggcacccc ggagggtagt
     3601 tcgccgggcg caacacccgg ctctcgccgc cgagcacagg gatgaattct tttttgtccc
     3661 aaatatggtt gcctccggtg aggcaggcca cccccaggga aaaaaggtcg ttcatcactt
     3721 tttccgtgag accaaagccc gccgccgcat tttcgccgtt ggccaggacg aagtcgaagg
     3781 gcccctcctg ttgccgaagc tccggcagcg cccgggccaa ggcctctcgc cccggacgcc
     3841 ccaccacgtc tcccacgaac agaaggcgca ttacttggcg tactccacgg cccggctttc
     3901 tcgcacgatg gtaacccgga tctggccggg gtatttcatt tcttcttcga tcttccgggc
     3961 gatatcgaag gccatcttgg cgatggaggc gtcatctgtc tggtggggag ccaccatgac
     4021 ccgcacctcg cgtcccgcct gaatggcaaa ggattttgtc acgccgtgga aggaattggc
     4081 cacgctttct agtttttcca ggcgcttgat gtaggcctcc aggctctccc tccgggcccc
     4141 ggggcgggag gcgctcactg cgtccgccgc cgccaccagc accgcgtgca cggtctgggg
     4201 ttcttcgtct tcatggtggg aggcaatggc gtttaccacg tcgggagctt cgccgtaccg
     4261 ccgggccagg tcggccccga tgagggcgtg gggcccttcc acctgatggt ccacggcctt
     4321 gcctatgtcg tgaagcagcc ccgcccgtcg ggccagggct tcgtccaccc ccagctccgc
     4381 cgccatgatg cccgcgacgt gggccacttc caggctgtgc atgagggtat tctggccata
     4441 gctcgtgcgg aactgcaact gtcccaccag cttggcgagc tcggggtgca tgttcttgac
     4501 ccccgcttcc agcagggcgt tttctccccg gtcgatcatc tcttggtcca cttcctggta
     4561 acacttctcc accaattctt ctatgcgggc cggatggatg cggccgtcgg tgataaggcg
     4621 ttccagagcc cgccgggata cctcccgacg caccggatcg aagctgctga tggtcaccgc
     4681 ctcgggggtg tcgtccacga tgaggtcaac ccccgtaagg gtctcgaagg cccggatgtt
     4741 gcgtccctcc cgaccgatga tgcgcccctt catttcgtct gaaggcaggg gcaccacgct
     4801 caccgccgcc tcggaggtgt gctccacgca ggtgcgctgg atcgccgtgg cgatgatctc
     4861 ctgggccttg cggtccgcct cgcgcttggc cttttcttcc agctctcgca ggcgcactcc
     4921 gatgcggtgg tttgcctctt cttccaccgc cgccagaagg atctgctggg cctcctcccg
     4981 ggtcatggcc gccacctctt ccagcttgcg ggtctgctcc gtgtacaggg agtccacgtc
     5041 ctccatgcgt cttttaaggg cctcttgccc cgcctttagc tcctcttccc gacgccccag
     5101 ggcatcgagc ttccgatcca ggttctcttc tttctgttcc agcctccgct cggcccgctg
     5161 catctcgttg cgacggtctt tgacttcttt gtcgatttcc tgtcgaagcc tcaggttctc
     5221 ctccctggcc tcggtgagga gttcgcgctt gcgctgctct ccctccgccg tcgcatcgcg
     5281 gagcagcgcc tcagaccgag ctcgggcatc gctcatggcc cgctcttccg tagttttgcg
     5341 aagcaggaag ccgccgccta tgcccgccgc gagccccgcc acaaaaaaca atacttccat
     5401 acgtcgtctc accccttatg gatttgtcaa agtacgcccc tttcccctca aaaggcgaaa
     5461 gtcgggaaaa actccgctct cattgtatcc ctttcttccc atccgctcaa ggggcaatcg
     5521 ccccggagac gatccggagc gctgccattt cggaggtcga aaacgagggg ccggaaaggg
     5581 gcggggggac gacatcgccc ggggacgcgc cttgggcccc cgggcgatgg atcactacgg
     5641 tctaaggcat cgatgatggg agacaaaacg cagggccacc cctgcggaaa gatccgaagg
     5701 gcctaggtaa attactagga atcggcgacc cggtctttga aggccttgcc cggtttgaaa
     5761 gctggcacgg tgcgggccgg aatctccacc acctggtccg gggcctgggg gtttcgcccc
     5821 tggcgagggg cacgaactcg ggcctcgaaa actccaaaac ccaccaagga gaccctcctc
     5881 cccccggcca cggcgcccat caccgcatcc aacacggcat ccacggccac cgtgacgtct
     5941 ttgatcttct gccccgtcga ctccgccacc tcgtggacca ggtctccctt ggtgaacgat
     6001 tctttctttt tcactgcgct acactccttt ttctgctaaa attaccgatc ctagtggggc
     6061 tttcccgaag ccccccccgg gtcatccccc gctccgtggg gcgcgttgcg gttgcgcccc
     6121 tccttagaaa agcgaggaga ggccccaccc aatccttcgt ccgacgagcc tctccagggc
     6181 agggggcctt tcttatcggg gtctttaccc aaggggatct ccgtccatcc ccaaggggta
     6241 agcagcacga aacgcaattc caccccttgg tccgccaaca tgcggacgtg gggcagatag
     6301 cgcagggcat cccccacgat ccagttgtca caaaaaaccc aaacccgatg gcactttccc
     6361 cgggcaaaga gggccgcgtg atccaccgcg atgcgcaacc cttcgtcgat gtctatggga
     6421 ccgatggcca gcacgagggc ttcgggccaa ccccgccccg gccctccgtt ttgcgtgggg
     6481 agaaggtcaa cgctctcctc cagccagctg agctgcaaaa agaggttctc cgtgtctccc
     6541 atcatccggg ggtcgacgcc ctccgccggg ttttcttctt cgtagaaggc ttcttcggcg
     6601 gccagctcgc ccatcatcca ctgcaacact tgctccgccg cccgcgtcac gaaaccgggc
     6661 caggaggaag actccatggc ccgcaggatg tctcccaccg ttaccggacg atccttaccg
     6721 tcgaaaaaac gtctgcgctg cattctctcc acctcctccg gcttcgaaac cccgatccct
     6781 ccgccagcat aacacaaggg cctccccttc gccacagatt tcctccgggg tttagatcca
     6841 aacggaaaaa atttttcacc caccggatct cttccgaagt aaatcccacc cggaagagtc
     6901 tataataaga ggcgccatat gccaaccatc accccccgaa tggaggagat tgtcccatga
     6961 aaccctttgt cctcgacgcc cgccgctccc tggtattgct catcgacatg caggaaaagc
     7021 tcatgcccgc catcgcccac cgggaggaga tcgaaaagac gggggcattg cttttgcgct
     7081 gcgcagacgc cctgggcgtg ccggtgttgg tttcggagca ctattccaag gggctgggga
     7141 aaaccctccc atccctggtc caggtccttc ctgaaaagag ccccgtggtg gaaaagatct
     7201 ctttttcttg ctggggagag cccgcgatcg tccggaccct ggaggagacg gcccggcgcc
     7261 aggtcatcct gctcggggtg gaaactcaca tctgcgtcct gtccaccgcc ctggagcttt
     7321 tggccgaggg ctacgacgtg gtgctggctt cagacgccac gggaagccgc cgggaagtcc
     7381 accgggacga ggccttcgcc gccctgcgct ccgccggagg gacggtgcta cccacggaaa
     7441 gcgtggtcta ccagctcctg ggccaggcgg gcaccgagct ctttaagcgc gtcctgcctt
     7501 ttttcaaagc gtagtcccat gccctcggcg gaacccctct ctggatatcc aaaaaatttc
     7561 attcgcttcc tcggcacggc gggggcccgc ttcacggtgt tacaccagat gcgggcctcc
     7621 ggggggctct gggtgcgcct gggaggggct tcctgggtgg tggacccggg gccggggtct
     7681 ttggtgcgca tctgcgaggc ccgccctccc ttggatccgg agtccctgga cgctctggta
     7741 ttgacccacc gccacatcga ccacagtacg gatctgaacg tcctggccga ggccctcacc
     7801 ggaggagggc gtcgaaggca cggttgcgta gtgctccccg aagacgccct ttcgggaccc
     7861 gaacccgtct ttttccgcca cgtgcgggac cgggtggatc gggtggagat ctggggcccg
     7921 gagaggggtg tcctcctccc cggaggtacg gagcttaccc cggtacccct ggaacaccac
     7981 ggggtcctgt gcttcggctt cgtggcccgc tccccagggc tccccacctg gggggtggtg
     8041 agcgacactc gccccttgcc ccacctggga aaactccttt cctcctgcga tatgatcgtc
     8101 gcgcacacca ccttgcgcca cgccgtggac accatcgacc acctctgcct ggaggacgcc
     8161 cggaccatgg cggaggtggc ctcccctcgc ctgatgatcc tcacgcacct ggggcgggga
     8221 atccttcaag aggatccccg ccgactggcc gaggagatgt cgtcggatcg cacgcgcttt
     8281 atcgcggcct gggacgggat ggtgctaaat ctggatacgc tggagccgga aagcccctac
     8341 tccgggacat gagaaaagcg tcttcccaag gaaaagagcc gttgtgttcg ccgataaaat
     8401 ccgctacaat tttcctccag gaagccgatg cagagtattt tgtatgggga ttttgtcccc
     8461 cggcgcccgg ggccgccggc aagaggcgct ccggcgcggc caggggccat gagggactcc
     8521 aaaggagggg atcgtcgtgc cgtacatcgc ccgccgcact cgggagcacc cccccggcgt
     8581 gccttttcgt ctgatcaacc tgctccggga gcttcgccat cagggggaag atctcatcga
     8641 tctcggatgg gggggtcccc atcgccttcc cgccccccac gtcttggcct ccgtggtcga
     8701 tgggtgccgc gccgaggacg ccttcagctc cccccatatc attccgtcgc tccccgaggc
     8761 gatcggagag tggtaccgga gccgtttcgg cgtatcgctg gaccgcaact ccgaaatcct
     8821 tcccgtctgg ggcaatctgg aggggttgct atcccttccc ttggccttcc tggacaccgg
     8881 agacggcatc ctccttccca atcctggaaa cccccagtac gtctttttgt cctccacggt
     8941 ggacgcccaa gtcaccttct atcctctctc cccggcgcga tcctttctcc cgtcctttga
     9001 ggccctaaag cccctcgtca ctccccgggt gaggcttttg gtgctccagc tcccctccga
     9061 tcccacggcc acggtggggt cacgggatct gttggcccag gccgtggact tctgtcgtcg
     9121 ccacgatctg gtgctggtcc acgaccgccg cctgggggag atcacggacg atggggtctc
     9181 ttcccccagc gtcttcgagg tccccggggc ccgggaggtc gccgtggagt acggttcctt
     9241 cacgtactcc tttcacatgg cgggggcccg attgggctac ttcgtggggg gatcccccct
     9301 catcgccgcc atggcgcgta cccgggggcc ctttcccttg ggccccttcc tccccatcca
     9361 gcgcgccgcc atccaggccc tcacccacag cgaccgggat gccgccatcg aaggcgtccg
     9421 ggtcctatac cgagatcgac gcaatgccct cctccaggcc ctggcctccg tgggatgggc
     9481 cattccccct tctccgtgtc ctctctacgt ctgggccccc gtgcccggag gggacgacga
     9541 tcgcttcgta gaacgttgcc tcatcgaggc gggggtggtc ttcacgccag gaaggacctt
     9601 tggcgacgcc ggaaggggtt acgtccgcat ggatctcagc aaagaggaac tcccccgcct
     9661 gatggaggcg gtggatcggt tacagcccct cctggtccct ctcttatcct gacctggagg
     9721 cgtcacaaaa tcccctgccc ttaggccccg ggagggcccc cgtcacgcct tcaaaggggc
     9781 ccaagaaagg agactgatgc tcgtgtacga tcgaattcga tccctggagc tacaccgccg
     9841 cgccagagga aaggtgaaaa tatttcccac cataagcgtc aacaacgaag aagacgcctt
     9901 tctcgcctcg tacccgggaa gcgtggcccc gggcatggcg attgtggaaa acaaggacat
     9961 ggtctacgag tacaccggca agggaaaccg tttggcggtg gtctccgacg gtagcgccat
    10021 ctgcggcatg ggaaatgtgg ggcccctggc gggaatgccc atgattgaag gcaaatgcct
    10081 gctgtttaag ctcttcggcg atgtgaacgc catccctctt tgcgtcagta gcgaaaaccc
    10141 cgacgagttc atccacatgg tttccatgat cgccccgaac ttcgggggga taaacctgga
    10201 agacatctct tctccgaaca ccttcaccat cctgaggaag ctggagcgca tgctggacat
    10261 gcccgtcttc tgcgacgacc aagatggcac cgccatgctg gtcgtcggag ctctgatcaa
    10321 cgccatgaag gtagtgggta aaaaactgga agacatccgc atcgtgttct gcggcgccgg
    10381 ggccgggggc atcgccgccg cccagctgct gctccacgcc ggagcccgca acctggtcct
    10441 gctgaacagc aaggggatct tgggacccca aaacccctcc atgaactacg tccaggaaga
    10501 actcgccgca cggaccaacc ccgaggggtt gacggggggg ttggaacaag tggtgcgagg
    10561 ggccgacgtc ttggtgggga tctcttcctc cggccttttt tccggcgacc tcatccggtc
    10621 catggctccg cgctccgtgg tgtttgcctt ggccctacca accccggaga tctcctacga
    10681 ggaagcggtg gccgccggag ccgccgtggt gggcaccgga ctgagccgca ccggagggaa
    10741 aaatcagctc acccacatgc tcgcctttcc cgggctgctg cggggcgcca tggacgtgcg
    10801 atccaaagac attccccagg agctcctcct ggcgggggcc caggccatgg ccagcgtggt
    10861 ggatccccgg cgtctttccc cccagaacgt ggtccccgac ctgttttgtg acgaaacgac
    10921 cccccgcgtg gcagaggccg tggcccaaag ggcggtggaa cttaacctag ctcaaaaccc
    10981 cctgcccccg ggggaggtct ataactccgc ctggcagcgc atcttcggga accgccaggg
    11041 aggaatctga tcccgtacat gagaacaggg gggcccgtca acgggccccc ctgccattat
    11101 ttcggtgctc ctccgccgtc ggaggaccca tgggggcctt aagggcggcc accctttaaa
    11161 gagacccctc cgggcagcgc cgtcacgccc tccatgagga gcagggccct cttgagagga
    11221 gtccccgtgg ggcctccgta ccccgccagc cccccgtcgg tccgcaccac ccgatggcag
    11281 ggcacgaaga gggcccaggg gtttttcctc atggcctgtc ccaccgcccg ggccccctcc
    11341 ggcaccccga cctcttccgc cacttggcga taggtccggg cccccccgac aggaatgcgc
    11401 cgcaccacgg cgtacacccg gccaaagaac gggggaacgc gacccaccag gggcactgcg
    11461 ggatcctccc ctcgccaaaa tcggatccac ccgtcccaga gccaggaggg gagtacgccc
    11521 tgcccctccg ccaggacagc ctcccccgaa gggatcagct tcccatggga aaggggagcg
    11581 agttccacca ggccctcgtc ctcccacgcc aagcgccaca cccccaccag tcccgggagc
    11641 tccgccctct gatgccccgt cacgacgtcg tctttcatgc cctagtcacc accacagtct
    11701 tgagattaag gccgccgatc cccccagggt cacccaaagg gagtggcgtt cgctgcgcca
    11761 aagcaccgct ctgtcgaagg ggcctcgcca ctttttcgag aaaggaggcc ggggaaagaa
    11821 catgggggtg cgatcccggt aggcccgaaa ggccggacca aacttctttt cgaggaaggt
    11881 ttcttcgtag gggatgatgg cccccccata gatcgcgagg aaggccaaaa ggaaaacccc
    11941 caggacccca ggggagttcg ccatgatcgc ccatcccaaa ccgatggcgg cgtttcccag
    12001 gtacaggggg tttctcacga aggcgtaggg accccaggtc accaactgga cggcgcccac
    12061 ctcttcgccc cggtagcgcc caatggtgcc tgcggcccaa aagcgcagga gctgcccacc
    12121 caccaaggga atcagcccta aaaagcatga ccggagcgac ggtcgggcgt accccagaat
    12181 gagcaggaaa aggatcgtcc agatccctcc tcgccacggg aaaattcgct ccccccaccg
    12241 agggagatca acctttgcca tcgtctcctc ccggacatcc tactttctct tcggcctttt
    12301 cttcggcctt ttcttggtcc atgagccggg aggccaagtc ccagccgatg tatctcatgg
    12361 ccacaaaggc ccccatgacg atgcccgtgt actccgtgaa aaatctccac ccaatggcca
    12421 ccactcccgc caagttccac gggaccagga gcttgtagag caccatggca cccccttcgg
    12481 ccactccgct ggcccccggc gtgggaacga agtacaggat gaacatgaac atggcctgta
    12541 ccaggacgca ctccacgaag tgcaccggct ggcccaagga ccagatgagg caggggagca
    12601 cggagaagat gcaaaatagg tagaggcaag agaccaggat cgcccccagg aaataccatt
    12661 ttcccgtggt acagaagacc cggatgttat tgttgtattc gtcgatctcc cggtggatgc
    12721 gacgcaccac ccggaggatg gaccggcgat gcaccacccc cgcgcggttg agccagaagg
    12781 tcagcacccc gcaccatcgc tttatgagcc gagggcgcac caggctcagg gtgatgagaa
    12841 tccaggctcc cacaacaaag accaagatcc agatgaacat gccctggata aaaccctgac
    12901 ctcgcaggat ttggggctcc accaagaggg tgacgggcag cgtcagtccc aggagaaaga
    12961 gggtcaccaa ggtccgaacc agggtgatgg ccaccccttt ccccagggat actccatggc
    13021 ggtagaggac gtacatctgg aagggacccc ctcctccctg catgggggtg atggcgcagc
    13081 cgaagtagtt gatccacgtg agcacgaagg cgagccggag gggaaggcgc tcccccgccg
    13141 cccgggagaa gaggcgaaag cgcgtggcgt cacaaaacca tatggcgccc accatcatga
    13201 gggccaggac tatgccctcc ttgcgggcgt tcatgaggat tttcatggtg tgacgatccg
    13261 cagaaaagga caggatcgcc accaccaccg cgatggatat gataagaaaa agggccactc
    13321 ctcgacgaaa tgtcatcggg agatcctctt tctaccggat ctcccgttga ctttcggggg
    13381 cgtgcctcgc cgatatcgcc tcactgcacg ctctccgccg tattgttcag ggccaccaag
    13441 taccccccca gacgcaccag ggtgggagcc aattcctccc gcagccggaa ggccaattcg
    13501 aagaacttct tctcctccat agaggagagc gcgtcccgga gcaccgccaa aaggcgtctt
    13561 cgcaccgcag aaagctcccc atcgccgatc tcgtcatccg agagggccag aaggacttgg
    13621 ctgcgcccca gcacctggag gacccaggtc gtaccctcgg agagctggga gagggccggg
    13681 aaggcctccg ggaggttctc ttcttccagg aggtcggcga ccttttccgc tcctttgtgc
    13741 agctccggga ggtaacggat ggcctgaccc agggactctc gcaccagctc ccgcaccgga
    13801 cagctcgtca ctcggagctc ctgccccccc gtcacctgca aaaaagcttc gacctccatg
    13861 gcctctccat ccacttccag ggcgcgacaa atccgcccca gggacgccaa atggccattc
    13921 agcgcctccc acagctcttc ctttgttccg gccacgggtc cttcacccca tgggacttcg
    13981 tcgatccaca ctcgcatcac aaaaaccccc ttcggagcct ggcaaggcca aaggggtcac
    14041 cccgcatagg ggaaaatccc tcccccgggg tcccttcggc cgtcggcaat ccccctcctt
    14101 aacaccgcaa actttcctca aggacctccc cgggccacag agcgtccaga tcaaaacacc
    14161 cgggcgtcga tctccaccac gggacctttt atccccggtc gccaaaggcc cgggggaaaa
    14221 ttagaagcgg cccgggtctc atcggtcttc ccctaccgaa aaaaggtccc tccgggggcc
    14281 ttgaggccct tggcgcttgt tttacggctt taccgccccc cgggaagcgg ggacgaagtc
    14341 ctcctccccc acggcatagc gtaagtcggc gtcggcggac caggcatcgt agatggcgtc
    14401 caccagcgcc gtgcgggcct ggttcagggc caatcgggca tccagcacgt ccaggttggt
    14461 gcctacctgg gcttcgtatc gcttcagggc catgcgataa tcctcttcgg cgctcgccac
    14521 ttggttatgg gccaccccca cacttgcctg agcggaggtc aggttgaaac gggccgtgga
    14581 cacctccagg cgcacctggc tttccaggtc tccgatgcgg gccaagagct cccgagccac
    14641 tccccgggcc tgatccaccc gggcggccac ttctcccgag tcgtagaggc gccattgagc
    14701 cgccaaggtg atcttccact catcctcctc ggggaagaac gaatctccct ggcgccccac
    14761 ttcgccctgg atcatcacct ggggcttggc cgcccccgcc gccgcctcgg caagaaagag
    14821 cgccgcctgc cgcgaggact ccagcatgcg gatctcgggc cgagaggcca gggcccggga
    14881 ctcggggtcg cccccttcgt gagggacggg agcgccttct tccccctccg ccagggtcca
    14941 ggagctccgt aggggagcgc ccaccgcccg ctcgagggcc gtccacgcca cgtccacggc
    15001 gttctgggcc cgaatcacgg cgagctcggc ccccgccaca tccacctcca cccgaaggac
    15061 ttcgtttttc gccaccacgc cgtttcggaa aaacgactcc acctgcttta ggtgctcctg
    15121 ggccagggaa cgggcttctt ctgccacggc taacttgtct cctgcccttt tcacgctgta
    15181 gtacgccctt cgggcgccgt tggccactcc ctgcagggta cggctccgct ccgcctcggt
    15241 ggctcgcacc ttccaccggg cggactggat cttcgcttcg atgctaccac cgctgaaaat
    15301 cacctgggaa agctggaggg ccaccttgta ggccgactca aaacccaggg gcacgacccc
    15361 cacggggttt cccgtgacgg ggtccagggc cacgctccgg ggggcctcgt tcacccgggt
    15421 ataggccaaa agggcgtcga gcttggggcc tccggcggcc cgggcttccc ggacctgggc
    15481 ctcatcctgg gatatctttt cttggagcac caaaaccatg gggttgtgct ccagggccaa
    15541 ggtcacggct ccatcggccg tgaggggtac ctccgacgcc ccgcttatcc cggcaaaagc
    15601 cccaatcccc acccatgcca gcaaaaccgc aacccatgcc ttttttctgt cgtgcactgt
    15661 gatctcctct cccgcccctc gtgccccggg gggcatcttc acccaaatat gccgcaccgc
    15721 ctgcctgggc gataaaagac cgccgctctc gcccgctccc ccaccgggac tccgccgcgc
    15781 ctcggaaaca gggtgtagaa aacgcccttc ggggggcttt gatgtcacgg cttctcgtcc
    15841 ccgaagaacg cgtggataat ctcccggatc tggcgctctc cttgggcaat ggcactttct
    15901 agagccgata ccggaagatc cagggccttc cacgcttcgt catccagcgc ggagacgaga
    15961 aacgatccgc tggacccccg gcgcatggcc ccggcgatga tgtccgccag gtggagcacc
    16021 gaggcgtcga aaagcgtggg agccttggtg gggttatggt gatagcgcac catgtccgcc
    16081 accacagggg gaagcttcca ctctgccagg aggagccccc ccaccgccgc atggctgaag
    16141 tcaaacacct ccccttcggc ctcaaagagc tgacacgacc gctcttttga tagatacagg
    16201 gcccgggtca tgtacttcgg gtagcggcga aaaaggacca aacggccgat gtcgtggagc
    16261 aaccccccca cgaaaaagcg ctcctcgtgg gaagatttct tcgagaaggc caacacccgg
    16321 gcatacaccc cgcaggccac ggagtgctcc cagaaggagc gcatgttcac gcaattcggc
    16381 ggtatgtctt taaacaggtt tatggccgat atgcccaggg ccagggtgga cagctcattg
    16441 gacccgatga tggccaccgc tcggaaaatg gagtcgatgc gggaaggaaa gccgtaaaag
    16501 gcactattga ccacccgcaa gagcttggcc gaaaggctgg tgtctttgct caccacctcc
    16561 gccacgtgac tggccgagct gcgaggggag tccagcacct cttttatgcg aaagtagatg
    16621 tccgggaaag agcacagctg gacttcccgc tgcaccagat cggaaagggc agggcgccgc
    16681 cctggagcgt ccagatccat tccttcgggc tgccccgggg gctcttcacc catcgccccg
    16741 gagtttttcg ccaactgctc cgccacccgg agggtacaaa ggcggcgcag ctcctctatg
    16801 gcctcgtggg agcgattagt ggccgcaaag aagcgggaca ccatcctctc ggccctctgg
    16861 cgccgctctt cttcctccgg ggacaggcga tcggtaccct cttcttctcc gcctccttcc
    16921 accaccagct cggtcacccc ccagaccttc atggtctgaa ggtagctcgc cttgatcacg
    16981 gctccggcgg gaaggattcg tcgcccgttg ggggccagga gatcctgggc caaaaccatg
    17041 ccttcgcgcg cttcgctcac ctgcatccgc accttgccca agaagtcatc ctctcccctc
    17101 catcccgcga aatccctggg aaaatgtccg aaaaaaacac tgccttccgc cctagccccg
    17161 ccaaagaaga ttactcatca tttgttcgtc agccggttgc catgacgtgc gctagccttt
    17221 ccgcaagtga cgatccagca tgtcttttaa ctcctggggg cgcaccggct tggataggta
    17281 gtcatccatt cccgcttcca ggcacatgtc ccggtccccg gacatggcgt gggccgtcat
    17341 ggccacgatg gggacgtggc ggcgaagccc ctcttcttct cggatgaccc gggtggcctg
    17401 aaaaccatcc atgacaggca tggaacaatc catgaggacc agatcgtagt cgacgaagcg
    17461 caggcgctcc accgcctcct ggccgttcac cgcctcgtcc gcctggaagc caaatttgcg
    17521 gaggatcccc tgggccaccg tccgattgac cttgttatcc tccaccagga ggatgcgaaa
    17581 agttccctcg ccctcttccc ggggaagttc gatcctccgg ggggaatctc gatcaggggc
    17641 aggatccgcc gcgccggtaa agggttggga tcgccccagc cgttccatga cattccacag
    17701 ggcgtgcccc cgtaggggtt tggtaagccg ctcttcggcc ccagctccgc cgttttccct
    17761 ttggtccccg ggagatgcca ccaataccac cgagggaagg gccatccccg gcatatccat
    17821 atcacggatg tcttcccaca cggagtccag gaagatgacg tggtacgaag ggcctcggct
    17881 cctcaattcc tggagcacaa gctggggaga tcgcccctcg acgcactcca ccccccagta
    17941 ggagagctgc tccgccaagg ccttcctcca cccctcccag gggagagcca acagggccct
    18001 ccgccccacg aaggccccct tgcccaggat ccggcgctct tcctcccctt cgcgctcgtc
    18061 ccgggggagg gagagggcca ctcgaaaggt cgaccccact cccggacggc tggagaccga
    18121 aatgcttccc cccatgagct ccagcagcat gcgggcgatg gccagtccca accccgtgcc
    18181 cccccgggcc cgagagctcg acccgtcagc ctgataaaat ttctgaaaca ggcgcccctg
    18241 ggtttcttcg tccatgccga tgcccgtatc cgtcacttcg atcacaaaag acgcccggtg
    18301 ttcgtccgat tccacgcagg ccaccgccac ctcgatccag cctcgctcgg aaaatttcac
    18361 cgcgttgccg cagaggttca ccagaatttg gcggagcctc cccccatcgc ccaccacgta
    18421 ccggggggcc tccggagcgt atcgcaccag gagctccaag cctttttccg ccgccttgac
    18481 ggaaaaaagc agccccacgc tctccaccac ctcccgcagg tcgaaggggg cgggaaaaat
    18541 ctccagcttt cccgcttcga tcttggagaa atccagcacg tcgttcagga tgtggagcag
    18601 agcccgggcc gatcccagga tgacgtccac ctggtccctc tggtcgtcgg tgaggtcggt
    18661 atcccgcagg agttccgcca tgccgatgac cccgttcatg ggggtgcgaa tttcgtgaga
    18721 catgttcgcc agaaagtcgc ttttggcccg gttggccttt tccgccacca tcgccaaccg
    18781 gttcgcggcc tcggtggcgg cctccagatc ccggttg
//
LOCUS       DAOOER010000036        18290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_3511_length_18290_cov_9.286427, whole genome shotgun sequence.
ACCESSION   DAOOER010000036 DAOOER010000000
VERSION     DAOOER010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 18290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18290
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3511_length_18290_cov_9.286427"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PL162_06125"
     CDS             <1..248
                     /locus_tag="PL162_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91634.1"
                     /translation="TMLISVVEERAIERGKEIGKEIGKEIGKEIGKEIGKEIGESRRA
                     LAVASRMLDAGEPREKILDYTGITSEELDRLVANRKN"
     gene            925..1001
                     /locus_tag="PL162_06130"
     tRNA            925..1001
                     /locus_tag="PL162_06130"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:959..961,aa:Met,seq:cat)
     gene            1004..1080
                     /locus_tag="PL162_06135"
     tRNA            1004..1080
                     /locus_tag="PL162_06135"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1038..1040,aa:Met,seq:cat)
     gene            1151..1969
                     /locus_tag="PL162_06140"
     CDS             1151..1969
                     /locus_tag="PL162_06140"
                     /inference="COORDINATES: protein motif:HMM:NF014671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HQF91635.1"
                     /translation="MTLPVFVSPNPDGEEGLSPTVSSSVLRWSKSQGPRTIQDTLMTE
                     RRMDLVVDGVPEVTVVFTPGMEREWALGYLLSSRRIGGLADVASLELRPGQILVALRR
                     ALPGVGPRGRLLHSGSSALHSPEVSSTDFPVFPPWRTTFDIVMSGIDWIQEEELFRAT
                     GAVHVVALLRPDGTRILRVSDVGRHNAADKALGGASLRGEDLGRTMAITSGRLPDDMV
                     FKFLGGGIPLVASVSAPTAEGVARARHNGMTLVGFCREERMNVYCGEDRLAPEE"
     gene            complement(2049..2135)
                     /locus_tag="PL162_06145"
     tRNA            complement(2049..2135)
                     /locus_tag="PL162_06145"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2100..2102),aa:Leu,seq:taa)
     gene            complement(2265..3257)
                     /gene="trmB"
                     /locus_tag="PL162_06150"
     CDS             complement(2265..3257)
                     /gene="trmB"
                     /locus_tag="PL162_06150"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HQF91636.1"
                     /translation="MTLASFIVSPAPGEVPRRWELEGFSEKPWVEIGFGNGEFLVHLA
                     REHPERLVVGIEMSLTSVLKAARRAQREDLANVRLLHGDARFLVRESFADEAVDRIYM
                     NFPCPWPKQRHARRRVTASGFASGLAAVLSLGGEFSLCTDEGWYAEEVRHILSRHPAL
                     EVLSFVVNPPHAVTTKYERKWLASGKDIFTVTFRKVSPWTERRLVKGAMEMHVEVPFR
                     EDLKARLPRLVGQGGGEEGTRWFFFEGFWGDDGVALVPVLANDEGFEQRFLLRIVPRP
                     STLLIKVDPVGSPYRTPAVAATLRAAARILAGPDAETALHGADGGAGPDQEEHA"
     gene            complement(3391..3972)
                     /locus_tag="PL162_06155"
     CDS             complement(3391..3972)
                     /locus_tag="PL162_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HQF91637.1"
                     /translation="MIPTREAALELFLRHNRDEMHLRHARALEVVMRHFAPRYGGDVD
                     LWGVVGLLHDLDWEETQHAPERHTADAIPLLRDEGYDEQIVRAMRSHSWGLLQDDVPP
                     VTPMEKVLYAVDELTGFVSAVALVRPSRSLSDLEPKSVLKKWKDKAFARGVDREVISR
                     GAELLNVPLEELISAVIEALRPVETELGLGTAR"
     gene            complement(3969..4502)
                     /locus_tag="PL162_06160"
     CDS             complement(3969..4502)
                     /locus_tag="PL162_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HQF91638.1"
                     /translation="MTSGTSELRSVLGGNPKDRPGWDLYFLSIALVVASRSTCLRRRV
                     GAVVVRQGHILSTGYNGAPRGVPHCDEVGCLRQQRAIPSGERHELCRGAHAETNAIVQ
                     AAATGTRTEGAEVYCTHEPCGLCTKVLINAGIVRIVYAEPYEDPVARELRRQAGVEVC
                     HKENPGGLWTFLEEVLS"
     gene            complement(4499..5185)
                     /locus_tag="PL162_06165"
     CDS             complement(4499..5185)
                     /locus_tag="PL162_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91639.1"
                     /translation="MEPAGILPYWNHDVELDVVSWLPYSFDPHPVSVQAMHSEELRAI
                     AAMALELGSELLYLNSLGSGQVGSLFPDRLIDPGRHEDAAEGFAMIFGYSRPMEMLTG
                     LLSSSVPGLKAGLALCGLWNRRMLLGEMKVIAFASQNILFDETPFLALLHEVLSKAAG
                     DRQRYAYYAEKFGYRRATIPFETDDMTQLAITQMLFDLEMEETRQVVGPAPEISWETP
                     WSSSLGGVSL"
     gene            complement(5185..6105)
                     /locus_tag="PL162_06170"
     CDS             complement(5185..6105)
                     /locus_tag="PL162_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HQF91640.1"
                     /translation="MRSEKILTEVGTNEWQVVVFLLGDQAFAINVDKTREILRWTGVR
                     AVPRSNESMLGIATVRGEVIPLIDLRSYLGIRSEILLEESKVIVAEFNKMKLGFVVDA
                     VERIYRINSDELDSTLSGSFLGENALYVIKRDGRNILLLDYERIVQAINPTFSENFHL
                     TSDVAHNVGQKIGDPNAFRILVAEDSPLIRKLIRDSLEQGGFHNVELVGHGKAAWDRL
                     QDEEDVFHLLITDIEMPKMDGLTLTRRVKEDPRLKGTPVVVFSSIMAEDIKRKAQSVG
                     ADVQITKPEIDLLVGKVCDLLLASTKGHRV"
     gene            6468..7196
                     /locus_tag="PL162_06175"
     CDS             6468..7196
                     /locus_tag="PL162_06175"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQF91641.1"
                     /translation="MKIRPVILCAFVAVVALYLGTPEELLASSRVISRAKVPGRGKVV
                     ALTIDDGPHSGYTERLLAILREAKVKATFFLVGRMAATTPELVRQELAEGHTLGNHSH
                     YHNNLLSLPPEGVVTEWRMCNYTVRSITGEFPRFCRPPGGNYDASVLEAATAEGLTAV
                     LWTANAADCTGLNSRDIAQKVLSQVSPGGIILVHDGVEATVQALPHILSTLKKRGYTF
                     VTLDEVLPVSSWAEQPSSSSSPSM"
     gene            complement(7274..8827)
                     /gene="malQ"
                     /locus_tag="PL162_06180"
     CDS             complement(7274..8827)
                     /gene="malQ"
                     /locus_tag="PL162_06180"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HQF91642.1"
                     /translation="MRDTLRRAGVLLHLSSLPSPFGVGDLGPGAYRFVEFLAQAGMGL
                     WQILPFCPTEGGRGNSPYSASSAFAGNPLFISPELLREMGLLTPEELAAVPSFSEDRV
                     DYDGVFRWKARCLDAAFARARRSPQIAGEVRQFLHGQRTWLQDYALFSALKKHFDGLP
                     WVAWPEVLRDRDAVALEAKRQELAWDVEREIFLQYIFFRQWGSLRAFCSAKGVHILGD
                     VPIFVDHDSADVWSHRDLFLLESDGSPSAVAGVPPDYFSPTGQRWGNPLYHWDNLRKT
                     SFQWWEDRLVHALCWCDELRIDHFRGFSACWSIPAQDATAERGEWRPVPGEELFQHLK
                     RRLGKLPFIAEDLGVITDDVRALMARFHLPGMKVLQFAFGGGFPENPYLPHHHVPDSV
                     IYTGTHDNNTTAGWFREEASKEVRDLLAEYLRYPVTDRTVHEAFLHMAFSSVARTAIV
                     PAQDIFGLGSEARMNTPSVPLGNWAWRLPIEFLRREGAQRLRRALFLGGRLAKVPYGG
                     GERLDSVKR"
     gene            complement(8820..10739)
                     /gene="glgB"
                     /locus_tag="PL162_06185"
     CDS             complement(8820..10739)
                     /gene="glgB"
                     /locus_tag="PL162_06185"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HQF91643.1"
                     /translation="MEAHPMVYGLSRLTDYDIYLFRGGNHWSLQDKLGAHETVEDGVP
                     GVHFAVWAPNAQNVSVVGDFNEWRPDVSPLKPRWDSSGIWEGFLPGGGRGMLYKYAIE
                     HPGGVSEKSDPFALRTEVPPRTASVVWSPAHEWQDEGWMRHRGHRNALSAPMSIYEVH
                     LGSWKRWEGRSLSYRDLAQDLVPYVKSMGFTHVELLPVMEHPFYGSWGYQCTSFFAPT
                     SRLGSPEDFMALVESFHREEIGVILDWVPSHFPTDGHGLGFFDGTHLFEYGDMRKGFH
                     QDWSSYVFDYGLGEVRSFLISSALFWLQRYHADGLRVDAVASMLYLDYSRQRGEWVPN
                     VYGGRENLEAMEFLRCLNTAIYERCPDVQTIAEESTAWPMVSRPTYVGGLGFGLKWNM
                     GWMHDTLAYFSRDPIHRKHHHDQITFSLMYAFSENFCLALSHDEVVHGKKSLLGRMPG
                     DEWQRAANLRLLLGYLFAHPGKKLLFMGGEFGQWQEWYHEHGLHWELLEYAFHAGIQR
                     WVRDLNAVYRSHPALYEQDFTWEGFSWVDCHDRDNGVLSFLRYGRQGETLLVVFNMTP
                     VPREHYRLGVPRSGWWKEILNSDGQVYGGGGVGNGGGLEGDDIPSHGYPASLSLTLPP
                     LGVLFLLEGGSPAHA"
     gene            complement(11014..11886)
                     /locus_tag="PL162_06190"
     CDS             complement(11014..11886)
                     /locus_tag="PL162_06190"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQF91644.1"
                     /translation="MRFFSEILMIGGALYLFFLGFLFLFGERLIFHPPSPPYSSEELE
                     FSLLPTPSGEKVAAVFFPEPDASAALIYSHGNAEDLAALLPRLERIASLGVSVLAYDY
                     PGYGASTGKPTESGVYEAAEAAMTFLQKQGYPPSRIFLMGFSLGSAPTIFLAHRHAVA
                     GIILQGAFLSAYRTVTLFPIFPGDRFVNEDRIASIVCPALFIHGTDDRVVPFWHGRSL
                     YDRYRGPKEGLWIEGGGHNGLWGHAPTQLSDALEAFFSRSLPQGTAPLRRRKQEGRLA
                     IKKKKRARRGVSKA"
     gene            complement(12077..13057)
                     /locus_tag="PL162_06195"
     CDS             complement(12077..13057)
                     /locus_tag="PL162_06195"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HQF91645.1"
                     /translation="MIEPGSAYLTVTGLKKYYPVRKGLFQGVVGHVKAVDDVSFFINE
                     GETLGLVGESGCGKTTTGRALLHLIEPTSGQVMLKQVDVGNAVKTDPGTMHRRMQIIF
                     QDPYGSLNPRMTVRDIVGEAVRYHGIADGKDLDAYVCDIMSKCGLRPEQRFRYPHEFS
                     GGQRQRVGIARALAVKPEFVVCDEPVSALDVSIQSQVLNLLVDLREQMKLTYLFISHD
                     LSVVRHISDRVAIMYLGQMVELAAKQEFFDRPLHPYTQALLSAIPVPDPTKARNRILL
                     EGDVPSPQNPPSGCRFHTRCRHAMDRCKQEIPSFANVGNDHFVACFLHQK"
     gene            complement(13050..14045)
                     /locus_tag="PL162_06200"
     CDS             complement(13050..14045)
                     /locus_tag="PL162_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQF91646.1"
                     /translation="MPLLELKNLKTYFDTDRGTVKAVDNVSFSIEPGQTLGVVGESGC
                     GKSVTALSIMRLVHKPVGRIAGGEILLHGKNLVGISEEEMRDIRGNKISMIFQEPMTS
                     LNPVLTVGSQIMEPLMLHQNLSKGDAKDRAIEMLRIVGLPNAEKRVDQYPHSFSGGQR
                     QRIMIAMALACNPALLIADEPTTALDVTVQAQILDLMNKLKKEFNSSIMLITHALGVI
                     AETAQRVVVMYAARVVEEADVKPLFGKPRHPYTQALLESIPRLDEERDRLNAIPGVVP
                     NPLDFPPGCRFHNRCSQCFDRCSVDEPPLFLLKDGRSVRCWLYETDKDKVGEIHD"
     gene            complement(14057..14947)
                     /locus_tag="PL162_06205"
     CDS             complement(14057..14947)
                     /locus_tag="PL162_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQF91647.1"
                     /translation="MTAQQIRKSGGSLWYEAWLRFRRNKLAMVGLFMVVSIVGIAIFA
                     PYVAPHDPIKQLIWTEGKEAKLAAPTTAHLMGTDLYGRDIMSRIIYGARISLQIGIFS
                     TLVSLLLGVPLGALAGYFGGKVDDFISWIINVIFAFPFLLFVLAVVAVFKNPSMVVVY
                     IAIGVVQWVSIARVVRAQFIYLREREFVEAAKALGLPTWRIVFVHILPNALAPVIVQA
                     TLGMGGIILLEAGLAFLGFGAQPPTPSWGLMISEGQRYLGQGIWWWAVFPGLAIMYTV
                     LGFNFLGDGLRDALDVRLKR"
     gene            complement(14959..15885)
                     /locus_tag="PL162_06210"
     CDS             complement(14959..15885)
                     /locus_tag="PL162_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQF91648.1"
                     /translation="MLSYILRRLLYTIPVVWGVVTVVFILINVVPGDPARLMLGQRGD
                     PATLAKIRSDMGLDLPIYQQYFNFLRDVAKGDLGKSYRSNEQVTTALLERFKATFRLA
                     LWAIILAALVGITAGVISAVKQYSAFDYSAMIIAISGVSAPVFWVGLLLLLAFAYNLN
                     WIPGVGYGDGDWRYLILPVITLGVRPAALLARITRSCMLEVLSQDYVRTARAKGLAER
                     VVVGRHAFRNALIPVITILGTQISDLLSGAVLTETIFAWPGIGRLEVEALINRDFPIM
                     RGGIIFMALIFLVANLIVDLSYGFIDPRVRYD"
     gene            complement(16012..17655)
                     /locus_tag="PL162_06215"
     CDS             complement(16012..17655)
                     /locus_tag="PL162_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQF91649.1"
                     /translation="MKKHGLILLTLALGLSALFASVAFADEPVMGGVLRWHEINDPPK
                     VDPAQVTDTTSSRVVYLMFDSLVENDADGKGVTPALAESWEGSDGGKVWTFHLRKGVH
                     FHKETEGQPTANGGREVKAADWKYSFERLVYENSPRAYFLDMVKGYQDFISRDETGAK
                     EWAGIKVVDDYTLQFELEYPFAPFVAVLAYNTFVVVPKEDAEKWGKEFNFHPVGTGPF
                     CFDKWDHDQKLATKRNPDYWRKDDAGRQLPYLDGVEIVVIPDPTITYEEYKKGNIDCY
                     RDIPDEFYEDVQANYKSVFQERPQLGTYYYGFNNEMGDFAGPEKLKLRQAFNYAVDRA
                     RISDLVLNGRFPAARGVLPPGFPGYNENLKGYEYNPEKAKELMKESGYPDGFEVELLV
                     NNNPRHRAVGEAIQAQVAELGIKMSIKVLDWGVMLDLCDRGEVNLFRMGWVVDYADPD
                     NFLYVLFHSSNKGAKGNYSRYSNPEFDKLVEQARVDTDYAKRMELYQKAEQILVDDAA
                     WLFLFHYSNSLLYQPWVKNIHMPAFGDYTTPLYNVWVQK"
     gene            complement(17966..>18290)
                     /locus_tag="PL162_06220"
     CDS             complement(17966..>18290)
                     /locus_tag="PL162_06220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HQF91650.1"
                     /translation="LGQDVPMTFIGQFGFKCGRNLDKFCNVQHREGPTGAPYVMDWTV
                     SALDARVISTLDVHTHIMFVGEVVGAAVIREGSPLTYADYHHVKKGKSHQYSPTSIFN
                     KVKNL"
BASE COUNT         3633 a         5686 c         5544 g         3427 t
ORIGIN      
        1 agacgatgct gataagcgtg gtcgaggaga gggccatcga gaggggcaag gagataggca
       61 aggagatcgg caaggagatc ggcaaggaga tcggcaagga gatcggcaag gagataggcg
      121 agagcaggag ggcccttgcc gtcgcctccc gaatgctcga cgccggcgag cctcgcgaga
      181 agatactgga ttacaccggc atcacttcag aagagctaga cagactggtg gcgaatagga
      241 aaaactagtc gtacattacg agcgaatgca agtcaacccg aacgcccttt ctgtcgcccc
      301 gaaggaccag atgttcccac tccctctatc tggccgagaa cggcttaaca tcggtggcca
      361 tttcgggtaa ggcctgcaga aaaaagagga gaccgtgcgt cacatgggat gcctatggag
      421 gcgatgaaat ccccggggga gatcttcact caggccgctg tggcgatgtt atatgagtat
      481 ttccggaggt atccctcgaa agattaacaa ggtggcgacg gcctgtctga tgtcagcggc
      541 ctgttcgcag tcccggcgga aacatattca caaattttta aaaaaatcca gaaatacttt
      601 gcgcactgtc atcgccataa tgacgtgcat aatgttgcgc ttatgtcata aaaattactt
      661 ttatgatttg ccgagctgtt atttcccacc actagtttgt gctgtttttt aatatttctt
      721 tatcactaat gattgccctc ggcagacttc ccgcaacatc caaggtttca ttgccatttc
      781 ctgcgctccg aaatacccgg cgagatgcgc accgaggggc cagaagggga tttcgccgta
      841 tgttcgaaaa atctgcaaaa atcgacgggg ctcccccttg acgaagctcc gcgcgtgtga
      901 cataattctc cctgttgtca gtggcgcggg gtggagcagt ttggaagctc gtcgggctca
      961 taacccgaag gtcacaggtt caaatcctgt ccccgcaacc aatggcggtg tagctcagtt
     1021 ggctagagca tgcggttcat acccgcagtg tcactggttc aattccagtc accgccacca
     1081 gtttttcctg tcgatggctc tttttggagc catcgatttt tttatttttt tctttcccgg
     1141 gaggatggcc atgacgttgc ctgtgttcgt ttcccccaac cccgatggcg aagaaggtct
     1201 ttcgcccacg gtgtcctctt ccgttttgcg ctggtcaaaa agccaggggc cccgcacgat
     1261 ccaggacacg ctgatgacgg agcggcgcat ggatctggtg gtggacgggg tgccggaggt
     1321 caccgtggtc ttcacccccg gcatggaaag ggagtgggcc ctggggtatc tgctttcttc
     1381 ccggcgcatc gggggcctcg cggatgtggc gtcgctggag ctccgccccg ggcaaatcct
     1441 cgtcgccctg cggcgcgctc ttccgggagt gggcccccgg gggcgcctgt tgcacagcgg
     1501 gagtagcgcg ctccactccc cggaggtctc ttccaccgat ttccccgttt tccctccgtg
     1561 gcgcaccacg tttgacatcg tcatgtcagg gatcgactgg attcaggagg aggagctctt
     1621 tcgggccacg ggggcggtgc acgtggtggc ccttcttcgt ccggacggca cgcgcattct
     1681 ccgggtctcc gacgtggggc ggcacaatgc ggcggacaag gctctggggg gggcgtcgct
     1741 gcgaggcgag gatctggggc ggacaatggc catcacgtcg gggcgccttc cggacgacat
     1801 ggtgttcaaa tttctcggag gggggattcc cctggtggcc agcgtttccg cccccaccgc
     1861 cgaaggggtg gcgcgggccc ggcacaacgg catgaccctg gtggggttct gtcgggagga
     1921 acggatgaac gtctactgcg gagaggatcg cctggcgccg gaggaatgat ctggggtgcc
     1981 agagaacaaa aaaaagccag aggcccgaag ggggttttcc ccttcgggcc tctgtttgta
     2041 gaaatcggtg gtgccgggcg cgggagtcga acccgcacgg gccatgggcc caagggattt
     2101 taagtcccat gcgtctacca ttccgccagc ccggcgcaag ggcattgtag acggtggctt
     2161 ccatatccgt caaggaaggg aacgaggctc atcccacttt ctcccttcgg aggggcaagg
     2221 cgggaaatgc cggggcgttc tccgctccgg aggcccgggg ggccttaggc gtgctcttcc
     2281 tggtcaggac cggcgccgcc gtcggctcca tggagagcgg tttcggcgtc cgggccggcg
     2341 agaatccggg ccgccgcccg cagggtggcg gcgacggcgg gggtgcggta ggggctcccc
     2401 acgggatcga ctttgatcaa aagggtggag ggacggggca cgatgcgcag gaggaagcgc
     2461 tgctcgaagc cttcgtcgtt ggccaacacg ggcaccagcg ccacgccgtc atctccccag
     2521 aagccttcga aaaaaaacca ccgggtgcct tcttcgcccc ctccctgccc caccagccgg
     2581 ggaaggcggg ctttgaggtc ttcccgaaaa ggaacttcca cgtgcatctc catcgctccc
     2641 ttcaccaggc gacgttctgt ccagggggat actttgcgaa aggtcaccgt aaagatgtct
     2701 tttcccgagg caagccactt ccgctcgtat tttgtcgtca ccgcatgggg agggttcacg
     2761 acaaaagaga gcacttccag ggccggatgc cgggagagga tgtggcgcac ttcttcggcg
     2821 taccatcctt cgtcggtgca aagggaaaac tctcccccca gagagagcac ggcggcgagc
     2881 cccgaggcaa aacccgatgc ggtgacccgg cgccgggcgt ggcgttgctt gggccacgga
     2941 cacggaaaat tcatataaat gcgatccacc gcctcgtcgg cgaagctctc gcgcacgaga
     3001 aagcgggcgt ccccgtggag aaggcggaca ttggccagat cttcccgctg ggcccgccgg
     3061 gcggccttga gcacgctggt gagagacatt tctatgccca cgacaaggcg ttcggggtgc
     3121 tcccgggcaa ggtgcaccaa aaactctccg ttgccgaagc cgatttccac ccagggtttt
     3181 tccgaaaaac cttccaattc ccaccgacgg gggacttccc ccggagcagg gctcacaatg
     3241 aaagaggcca aagtcatcct caacacgtcc catccttttc tatgacttga cggcctttcc
     3301 agccggtccc ccgcacaacg aaacccccct tcggcactcc aggggggatg gggtggaacg
     3361 cggccgaggc ggcccttggg cgtggcgcgc ctaccgggcg gtccccaatc ccaattcggt
     3421 ctccacgggg cgcagggcct ctatgacggc gctgatgagc tcttccaggg ggacgttcag
     3481 gagctcggct cctcgggaga tgacttcccg atccacccct cgggcaaagg ccttgtcctt
     3541 ccacttcttt agtacggact tcggctccag gtcgctcagg gagcggctcg gacgcacgag
     3601 ggccaccgcc gagacgaagc ccgtgagttc gtccacggcg tagagcactt tttccatggg
     3661 cgtcacgggg gggacgtcgt cctggagcaa cccccaggag tgggaccgca tggcccggac
     3721 gatctgttcg tcgtatcctt cgtcccggag caggggaatg gcgtccgccg tatggcgctc
     3781 cggagcatgc tgggtttctt cccaatccag gtcgtgcaag agccccacca cgccccagag
     3841 gtccacatcg cctccatagc ggggggcaaa gtgccgcatg accacctcca gcgcccgggc
     3901 atggcgcagg tgcatttcgt cacggttatg tcgaagaaaa agttccaatg ccgcttctcg
     3961 agtgggaatc atgacaaaac ctcctccaaa aacgtccaca gcccccccgg gttttccttg
     4021 tggcacacct ctacccccgc ctggcgccgg agctcccggg ccaccgggtc ttcgtaaggt
     4081 tcggcataga cgatgcggac gatgccagcg ttgatcaaca ccttggtgca aaggccgcag
     4141 ggttcgtggg tacagtagac ctccgcgccc tcggtacggg tgcccgtggc ggcggcctga
     4201 acgatggcgt tggtctccgc gtgggccccc cggcagagtt cgtggcgctc tcccgaggga
     4261 atggcccgct gctgccggag gcatcccacc tcgtcgcaat ggggaactcc ccggggggcc
     4321 ccgttatacc ccgtgctcag gatgtgaccc tggcgcacca ccaccgcccc cactcgccgc
     4381 cttagacagg tgctacggga ggccaccacc aaggctatgg acaggaagta gaggtcccac
     4441 cccggccggt ccttcgggtt cccccccagg acggagcgga gctccgaggt ccctgaagtc
     4501 acagggagac gccccccagc gaggaggacc aaggggtctc ccaggagatc tccggggcgg
     4561 gtcccaccac ctggcgggtt tcttccattt ccaggtcaaa aagcatctgg gtgatggcta
     4621 gttgcgtcat gtcatccgtc tcgaagggga tggtcgcccg gcgatagccg aacttctccg
     4681 cgtagtaggc gtagcgctgc cgatcccccg ccgccttgga caggacctcg tgcaggagcg
     4741 ccagaaaggg ggtctcgtcg aagaggatgt tctggctggc gaaggcaatg actttcatct
     4801 ctcccaggag catgcggcgg ttccataggc cacagagggc cagtcccgcc ttcagccccg
     4861 gcacggagga ggagaggagc cccgtgagca tctccatggg gcgcgagtag ccgaagatca
     4921 tggcgaagcc ctccgccgca tcttcgtggc gcccggggtc gatgagccgg tccggaaaga
     4981 gggagcccac ctggccgctt cccaggctgt tcaggtaaag gagctcgctc cccaactcca
     5041 gggccatggc cgcgatggca cgaagctcct cgctgtgcat ggcctgcacc gacaccggat
     5101 gggggtcgaa ggagtaggga agccacgaca ccacgtccag ctccacgtcg tggttccaat
     5161 agggcaggat ccccgcaggc tccatcagac gcggtggccc ttggtggagg ccagaaggag
     5221 gtcacacacc ttgcccacca ggaggtcgat ctccggcttg gtgatctgga cgtcggcccc
     5281 cacgctctgg gccttgcgct tgatgtcttc ggccatgatg gaggaaaaga ccaccaccgg
     5341 cgtgcccttg agccgagggt cttctttcac gcggcgggtg agggtgagcc cgtccatctt
     5401 gggcatctcg atgtcggtga taagcaggtg gaacacgtcc tcttcgtcct ggagccgatc
     5461 ccaggccgcc ttcccatgcc ccaccagctc cacgttgtgg aatcccccct gctccaggga
     5521 gtcccggatc agcttgcgaa tcagcgggga gtcttcggcc acgaggatgc gaaaggcatt
     5581 gggatcaccg attttctgtc ccacattatg ggccacgtcg ctagtcaggt gaaagttttc
     5641 gctgaacgtg gggtttatcg cctggacaat gcgctcgtag tccaaaagca ggatgttccg
     5701 gccatctcgc ttgatcacgt agagggcgtt ctcccccagg aaagatcccg aaagggtgga
     5761 atccagctcg tcggaattga tgcgatagat tcgctccact gcgtccacca cgaagcccag
     5821 cttcatttta ttaaattctg ccacgatgac tttgctttct tccagcagga tctccgaccg
     5881 gatccccaga taggagcgga ggtcgataag ggggatgact tctccccgca ccgtggcaat
     5941 gcccagcatg gattcgttac tccggggcac ggcccggacg ccggtccacc gcaggatctc
     6001 tcgggtcttg tccacgttga ttgcgaaggc ctgatcacct agaagaaaaa ccaccacctg
     6061 ccactcgtta gttcccactt ccgtgaggat cttctcactt ctcataccga ttcccccccg
     6121 atcgcatgcg tcatttccgc cttccaccgc cacactatag atcatctttc ctgaaggctc
     6181 aagggcttcg tacaaaagaa aaaatcctct ttggtaaaac cccgcaccta caagtcgttt
     6241 cctcccagcc cggagcgccc ctcggagaca acgatggaac tgaaatcttg gagtctgtgg
     6301 acggcaaagg cccgacctat ggtctgcgac cagccactgg caaggacgac gcagatcaat
     6361 cccttccctc aacgggatcc atggaggaaa atccctccgc cccttgactt cctggcctcc
     6421 tgtcggtagt ctcaggggac atttgtatca tcgaaggagc gtgtccattg aaaatccgtc
     6481 cagtgatcct ctgcgcgttt gtggccgtgg tggccctcta tttggggacc cccgaagagc
     6541 ttttggcctc ctctcgggtc atctccaggg caaaggttcc cggccgagga aaggtggtgg
     6601 ccctcaccat cgacgacggt ccccactcgg gctacacgga acgcctccta gccatcctcc
     6661 gggaggcgaa ggtcaaggcc acgttcttcc tcgtgggccg catggccgcc accacccccg
     6721 agctggtccg gcaggagctg gccgaggggc acaccctggg caatcactcc cactaccaca
     6781 acaaccttct ttctctgcct cccgagggcg tggtgacgga gtggcgcatg tgcaactaca
     6841 ccgtgcgatc catcacggga gagtttcccc gcttctgtcg ccccccgggg ggaaactacg
     6901 acgcctcggt gctggaggcc gccaccgccg aaggacttac cgccgtgctt tggaccgcca
     6961 acgccgccga ctgcacgggg ctcaactccc gggacatcgc ccagaaggtc ctctcccaag
     7021 tctctcccgg ggggatcatc ctcgtgcacg acggcgtgga ggccacggtg caggccctcc
     7081 cccacatcct atcgacgctg aagaaaaggg gatacacctt cgtcaccttg gacgaggttc
     7141 tccccgtgtc ctcgtgggcg gagcagccct cttcgagctc ttccccctcc atgtgaggcc
     7201 tagactcctc ccctccgtgg caatctaagg ccacgactcc cgagggggcc ccagggtccc
     7261 ctcgggagtt tttctaccgc ttcaccgaat caagtcgttc ccccccaccg tagggcacct
     7321 tggccaggcg tcctcccagg aagagggccc gtcgcaagcg ctgggcccct tcgcgcctaa
     7381 ggaattcgat gggaaggcgc cacgcccaat tccccagggg aacggaaggg gtgttcatgc
     7441 gagcctcgct tcccaaacca aagatgtcct gggcggggac tatggcggtg cgggccaccg
     7501 aggagaaggc catgtgcaga aacgcctcat ggacggtgcg atccgtcacg gggtagcgca
     7561 ggtactccgc caaaaggtct cggacttcct tcgaggcctc ctctcgaaac caccccgccg
     7621 tggtgttgtt gtcgtgggtc ccggtgtaga tcacgctatc cggcacgtgg tggtggggca
     7681 ggtaggggtt ttccggaaac ccccccccga aggcaaactg aagcaccttc atgccgggaa
     7741 ggtggaaccg cgccatgagg gcccgcacgt cgtcggtgat gactcccagg tcttcggcga
     7801 taaaggggag cttacccaag cgtcgtttca ggtgttggaa gagctcctct ccggggacgg
     7861 gcctccactc gccgcgctcc gccgtggcat cctgggcagg gatgctccaa caggcggaaa
     7921 atccccggaa gtggtcgata cgcagctcgt cacaccagca cagggcatgc accaagcgat
     7981 cctcccacca ctggaacgat gtctttcgca ggttatccca atggtagagg gggttgcccc
     8041 aacgctgccc cgtggggcta aagtaatccg gcggcacccc tgccaccgcc gagggggatc
     8101 cgtcgctttc caacaagaaa agatcgcgat ggctccacac atcggcgctg tcgtgatcca
     8161 caaaaatcgg cacgtctccc aggatatgca ctcccttggc ggagcagaac gcccgcaggg
     8221 atccccactg gcgaaagaag atgtactgta ggaaaatttc cctctccaca tcccaggcca
     8281 gctcctggcg cttggcctcc agggcaacgg catcccggtc gcgcagaact tcgggccaag
     8341 ccacccaggg gaggccgtca aagtgctttt ttagagccga aaaaagggca tagtcctgga
     8401 gccaagtcct ctggccatgg aggaactgcc ttacctctcc ggcaatctgg ggagatcgcc
     8461 gggcccgggc aaaggccgca tccaggcagc gcgccttcca ccggaacacg ccatcgtagt
     8521 ccactcgatc ctccgaaaag gaagggactg cggccagctc ttccggcgta agtaacccca
     8581 tctccctcag gagctcgggg ctgataaaaa gggggttccc cgcaaaggcg gaagaggcgc
     8641 tgtacggcga gttcccccgc cccccctccg tggggcaaaa gggaaggatc tgccaaaggc
     8701 ccatccccgc ctgggccaaa aactccacga aacggtacgc ccccggcccc aggtccccca
     8761 cgccaaaggg ggagggaagg gaggaaagat gaagcaacac tccggcgcga cgcagcgtgt
     8821 cacgcatggg caggactacc tccctccagc aggaagagca cccccagggg aggcagggtg
     8881 agggagagcg aggcggggta gccgtggctg gggatgtcgt ctccctcaag cccccctccg
     8941 ttgcccacgc cgccgccgcc gtagacctgg ccatcgctgt ttaggatctc cttccaccat
     9001 ccgctccggg gcactcccaa ccggtagtgc tcccgaggga caggagtcat gttaaagacc
     9061 accaaaaggg tctccccctg acggccgtag cggaggaacg acaggacgcc gttgtcccgg
     9121 tcgtgacaat ccacccagga aaaaccctcc caggtaaagt cctgttcgta aagcgccgga
     9181 tggctccggt acacggcgtt caggtcccgg acccaccgct gtatcccggc gtgaaaggca
     9241 tactccagga gctcccagtg gagcccatgc tcgtggtacc actcctgcca ttggccaaat
     9301 tcccccccca tgaagaggag ttttttcccc ggatgggcga aaagatatcc caataagagc
     9361 cggaggttgg ccgcccgctg ccactcgtcc ccgggcatgc gccccaggag ggacttcttg
     9421 ccgtgcacta cttcgtcgtg ggaaagggcc aggcagaaat tctccgagaa ggcgtacatg
     9481 aggctgaagg taatctggtc gtggtggtgt tttcggtgaa taggatcccg ggagaaatac
     9541 gccagggtat cgtgcatcca ccccatgttc catttgaggc caaatcccag gccgccaacg
     9601 taggtggggc gggaaaccat gggccaggcg gtggactcct ccgcgatggt ctggacgtcg
     9661 gggcatcgct cgtagatggc cgtattgagg catcgcaaaa actccatggc ctccaggttt
     9721 tctcggccgc cgtagacgtt gggcacccac tcccctcttt gacgggagta gtcgaggtac
     9781 agcatggacg ccaccgcgtc cacccgtaga ccgtccgcat ggtaccgctg caaccagaag
     9841 agggcgctgc tgatgagaaa gctccgcacc tctcccaggc cataatcgaa aacatagctg
     9901 ctccagtcct gatgaaaccc cttgcgcatg tcgccgtatt caaagaggtg ggtgccgtca
     9961 aaaaaaccca gaccgtgacc atcggtgggg aagtgggagg gcacccagtc caggatgacg
    10021 cctatttcct ctcggtggaa ggattccacc agggccatga agtcctcggg ggatcccaag
    10081 cgagaggtgg gggcaaagaa gctcgtgcac tggtaccccc aggagccata aaaggggtgc
    10141 tccatcacgg ggaggagctc cacgtgggta aagcccatgg acttcacgta gggcactaga
    10201 tcctgggcca ggtcgcggta ggataaagac ctcccctccc agcgtttcca ggatcccagg
    10261 tgcacctcgt aaatgctcat gggggccgac agggcgttgc gatgcccccg gtgcctcatc
    10321 catccttcgt cctgccactc gtgggcggga ctccacacca ccgatgccgt ccggggaggg
    10381 acttccgtgc gcagcgcgaa gggatcggac ttttcgctca cgcccccggg gtgctcgatg
    10441 gcgtatttgt acaacatgcc ccgcccccct ccgggaagaa atccctccca gatgcccgag
    10501 ctgtcccacc ggggcttcaa cggagagacg tcgggacgcc attcgttaaa atctcccact
    10561 acagagacgt tctgggcgtt gggggcccat acggcaaagt gcacccccgg caccccatcc
    10621 tccacggtct cgtgggctcc cagcttatcc tgaagagacc agtggttccc tcctctgaaa
    10681 aggtaaatgt cgtaatccgt cagtcgagac aagccgtaga ccattgggtg cgcttccatg
    10741 ataccgccct ccctcatgcc ccgtcctcgg cgaaacgccc cgggagcctc cctgggccct
    10801 ctggtgaacg ccccacgcca tgatctaaaa aactctgcgt atgaccccgc tacatcctgc
    10861 cccatggtac tcgtttttcc gccgccctcc aagggggatg gggcttgaaa caaaaggttt
    10921 ctctccccct gggggaccgg tcacatcccc cggcgccggg ccggacccct tggtcgtgac
    10981 cccgccccgc cttcagagcc cccggggccc atatcaggcc ttcgacacgc cccggcgcgc
    11041 ccttttcttt ttctttatcg ccaggcgtcc ctcctgtttc ctccggcgca ggggcgccgt
    11101 cccctgggga aggctccggg agaaaaaggc ctccaaggcg tcggaaagct gggtgggagc
    11161 gtgtccccag aggccgttat gtccccctcc ttcgatccat agtccctcct tggggccccg
    11221 ataccggtcg taaagagagc ggccgtgcca gaaagggacc acccgatcgt cggtgccgtg
    11281 gatgaagagg gcgggacaca cgatggaggc gatgcgatct tcgttgacga agcggtctcc
    11341 gggaaagatc gggaaaagcg tgaccgtcct gtaggccgaa aggaacgccc cctgcaaaat
    11401 gatccccgcc acggcatgac gatgggccaa aaagatcgtc ggcgcactac ccagggaaaa
    11461 acccatcaga aaaatcctcg acggggggta tccctgcttt tgcaaaaagg tcatggcggc
    11521 ctccgccgcc tcgtagacgc cggattccgt aggtttcccc gtgctggcgc cgtagccggg
    11581 gtagtcgtag gccaagacgg acacccccag ggaagcgatg cgctcaagtc tcggcaaaag
    11641 cgccgccagg tcttcggcgt tgccgtggga gtaaatcagc gccgccgagg catcgggctc
    11701 gggaaaaaag accgccgcca ccttctcccc cgagggcgtg ggcaagaggg agaactccag
    11761 ctcctccgaa gagtaggggg ggctaggggg gtgaaagatg aggcgctctc cgaaaagaaa
    11821 gagaaacccc aggaaaaaga ggtagagagc cccccctatc atcaaaattt ccgagaagaa
    11881 cctcacgaaa atcctcctgt cttttccgat cgaaaccaca ccccccggaa gccctcgcgc
    11941 cccactctag ggccttctag agatcgttcc ccgtagagcc tggctctctc catcgcctcg
    12001 gacacgccaa aaaaagtagg gaggcgaggc ctccctactc taccagaagc gttctccttt
    12061 aggaaacgtc tgcggattac ttctgatgga gaaaacaggc cacgaagtgg tcgtttccca
    12121 cgttggcgaa ggaagggatc tcctgcttgc accgatccat ggcgtggcgg caacgggtat
    12181 ggaagcggca ccccgaaggg ggattctgag gggagggcac gtccccttcc aggaggatgc
    12241 ggttacgggc cttggtagga tcgggcaccg gaatggcgga aaggagcgcc tgggtgtagg
    12301 ggtgcagtgg gcggtcgaag aactcctgct tggcggcgag ctccaccatc tggcccagat
    12361 acatgatggc cacccgatcc gagatgtgcc gcaccaccga gaggtcgtgg gagataaaca
    12421 gataggtcag tttcatctgc tcccgcaggt ccaccagcag gttcaacacc tgactctgga
    12481 tggacacgtc cagggcggac acgggttcgt cgcatacgac gaactcgggc ttcaccgcca
    12541 gggctcgggc tatgcccaca cgctggcgct ggccgccgga gaactcgtgg ggatagcgaa
    12601 aacgctgctc cggccgaagg ccgcacttgg acatgatgtc gcagacgtag gcatcaagat
    12661 ctttcccgtc ggcgatgccg tggtaacgca ccgcctcgcc cacgatgtcc cgcaccgtca
    12721 tgcggggatt caggcttccg taggggtcct ggaagatgat ctgcatccgg cggtgcatcg
    12781 tcccggggtc ggtcttcacc gcattgccca cgtccacctg cttgagcatg acttggccgc
    12841 tggtgggttc gatgaggtgg agcagggccc gtcccgtggt ggttttaccg caccccgatt
    12901 cccccaccag gccaagggtc tctccctcgt taatgaaaaa cgacacatcg tccacggcct
    12961 tgacgtgccc cacgaccccc tggaaaaggc ccttgcgcac agggtagtac ttcttgagcc
    13021 ccgtgacggt gagataggcg ctgccgggct caatcatgga tttcacccac cttatccttg
    13081 tctgtttcat agagccaaca ccgcacgctc cgcccgtcct tcaacaggaa aaggggcggc
    13141 tcgtccaccg aacaacggtc gaaacactgg ctgcaccggt tatggaagcg acaccccgga
    13201 gggaaatcca gggggttggg gaccacgccg gggatggcgt tgaggcgatc ccgctcttcg
    13261 tccagccgag gaatggattc caggagggcc tgggtgtagg gatgccgggg cttcccgaag
    13321 aggggtttga catccgcttc ctccaccacc cgggcggcgt acatgaccac cacgcgctgg
    13381 gccgtctcgg cgatcacccc cagggcgtgg gttatgagca tgatggagga gttgaactct
    13441 ttcttgagct tgttcatgag gtccaggatc tgggcctgca ccgtcacgtc cagggccgtg
    13501 gtgggttcgt cggcgatgag gagcgccggg ttgcacgcca gggccatggc gatcatgatc
    13561 ctctggcgct gtccccccga aaaggagtgg gggtactggt ccaccctctt ttccgcattg
    13621 ggcagcccca cgatccgcaa catctcgatg gcccggtcct tcgcgtcgcc cttggagagg
    13681 ttctggtgga gcatgagggg ctccatgatc tgggatccca ccgtgagcac cgggttcagg
    13741 ctggtcatgg gctcctgaaa aatcatggaa atcttgttgc cccggatgtc tcgcatctct
    13801 tcttccgaga tgccgacgag gttcttgccg tggagcaaaa tctcccctcc ggcaatacgc
    13861 cccacgggtt tatgcaccag gcgcatgatg gagagggccg tgacgctctt gccgcacccc
    13921 gactccccca ccacccccag ggtctgcccc ggttcgatgg aaaaggagac gttgtccacg
    13981 gccttcaccg tgccgcgatc cgtgtcgaaa taagttttta ggttttttag ttccaacagg
    14041 ggcatgggaa tacctcctac ctcttcaggc ggacgtcaag ggcgtcgcgg agaccgtcgc
    14101 cgaggaagtt gaatcccagc acggtgtaca tgatggccaa tccaggaaag accgcccacc
    14161 accagatccc ctgcccaaga tacctctgcc cctcggagat catgagcccc cagctggggg
    14221 tcgggggctg ggcgccgaag cccaggaagg ccagcccggc ctccaggagg atgatccctc
    14281 ccatgcccaa ggtggcctgc acgatgaccg gcgccagggc attgggcagg atatgtacga
    14341 acacgatgcg ccacgtgggg agccccaggg ccttggcagc ttccacaaat tcgcgctccc
    14401 tcaggtatat gaactgggcc cgaaccaccc gggcgatgga gacccactgc accaccccga
    14461 tggcgatata gaccaccacc atgctggggt tcttgaacac cgccaccacg gccaggacga
    14521 agaggaggaa gggaaaggcg aagatgacgt tgatgatcca ggagatgaaa tcgtccacct
    14581 tgcccccgaa gtaccccgcc agggccccca ggggaacccc caacaagagg gagaccaagg
    14641 tggagaagat gccgatctga agggaaatgc gagctccgta gatgatccga ctcatgatat
    14701 cccgaccgta gagatcggtc cccatgaggt gggccgtcgt gggagccgcg agcttggcct
    14761 ccttaccctc ggtccagatg agctgcttga tgggatcatg gggggccaca tagggggcaa
    14821 agatggcgat tcccacgatg gaaacgacca tgaaaagccc caccatggcg agcttatttc
    14881 gccggaagcg gagccaggcc tcgtaccaga ggcttccacc gctttttcta atctgctgtg
    14941 ctgtcatgat ccccatcctc agtcgtaacg gacccgcggg tcaatgaagc cgtaggagag
    15001 gtcgacgata agattcgcca ccaggaatat gagcgccatg aagataatgc ctcctcgcat
    15061 gatgggaaag tcccggttga tgagggcttc cacctcgagg cggccgatgc cgggccaggc
    15121 aaagatcgtt tccgtcaaca ccgcaccgga gaggaggtcg gaaatctggg ttcccaggat
    15181 ggtgatcacg gggatgaggg cgttgcggaa ggcgtggcgc cccaccacca ccctctcggc
    15241 caaccccttg gcccgggcgg tgcgcacgta gtcctggctc aggacttcca acatgcagga
    15301 gcgggtgatg cgggccaaaa gggccgccgg gcgcacccct agggtgatca cggggagaat
    15361 gagataacgc cagtcgccgt cgccataccc cacgccgggg atccagttca ggttgtaggc
    15421 aaaggccagc aagagcaaaa gccccaccca gaagaccggc gcgctgactc ctgatatggc
    15481 gatgatcatg gctgaatagt caaaggcaga atactgcttt acggcggaaa ttaccccggc
    15541 ggtgatgccc acgagggccg ccaggatgat ggcccagagg gccagccgga aggtggcctt
    15601 gaaccgctca agaagggcgg tggtcacctg ctcgttgctc ctataggact tgcccaggtc
    15661 tcccttggcc acgtctcgga ggaagttgaa gtactgctgg tagatgggaa ggtcgagccc
    15721 catgtctgac cggatcttcg ccagggtggc ggggtctcct cgctgtccca gcatgagccg
    15781 cgccgggtcc cccggtacca cgttgattag tatgaacacc acggtgacca cgccccagac
    15841 cacggggatg gtgtaaagca accgtcgaag gatataggac agcatctttt cctctcctca
    15901 cctgtcgttc gcacgttagt cccctgagaa aggggccggg tgcggcccga agaagccaca
    15961 cccggcgatc cttccgccga aaaacccctg tatctacccc gatcggggac gctatttctg
    16021 gacccacacg ttatagagcg gcgtggtgta gtccccgaag gcgggcatat ggatgttttt
    16081 cacccagggc tgatagagga ggctgttgga gtagtggaag aggaagagcc aggccgcgtc
    16141 gtccaccagg atctgctcgg ccttctggta gagctccatg cgcttggcgt agtccgtatc
    16201 cacccgggcc tgctccacca gcttgtcgaa ctcggggttg gagtaccggg agtagttccc
    16261 cttggcaccc ttgttggagg aatggaagag tacatagagg aagttgtccg ggtcggcgta
    16321 gtccaccacc cagcccatgc ggaagaggtt gacttcgccc cggtcgcaga ggtcgagcat
    16381 gacgccccag tccagcacct tgatggacat cttgatgccc agctcggcca cctgggcctg
    16441 aatggcctcg cccacggcgc ggtgccgggg gttgttgttc accaggagct cgacttcgaa
    16501 accatcggga tagcccgatt ctttcatgag ttccttggcc ttttcggggt tatattcgta
    16561 gcccttgagg ttctcgttgt atccggggaa tccggggggc aacacgcccc gggcggcggg
    16621 gaatcggccg ttgagcacca agtcgctgat acgggcccgg tccacggcgt agttgaacgc
    16681 ctggcggagc ttcagtttct cggggcccgc gaagtcgccc atctcgttgt tgaaaccata
    16741 gtagtacgtg cccagctggg gtctctcctg gaagacgctc ttgtagttcg cctgaacatc
    16801 ttcgtagaac tcgtccggga tgtcgcggta gcagtcgatg ttgccctttt tgtactcttc
    16861 gtaggtgatg gtggggtcgg gaatgaccac gatctccacg ccatctaggt agggaagctg
    16921 ccgccccgcg tcgtctttgc gccagtagtc ggggtttctc ttggtggcga gcttctggtc
    16981 gtgatcccac ttgtcgaagc agaagggccc cgtgcccacg gggtggaagt tgaactcctt
    17041 gccccacttt tccgcatctt ccttgggaac caccacgaag gtgttgtaag ccagcaccgc
    17101 cacgaagggg gcgaaggggt attctagctc gaactggagc gtgtagtcgt cgacgacttt
    17161 gatccccgcc cactctttgg caccggtctc gtcgcggctg atgaagtcct gatatccctt
    17221 caccatgtcg aggaagtagg cccgggggga gttctcgtac accaggcgct cgaaggagta
    17281 cttccagtcc gccgccttga cttcccggcc gccgttggcg gtgggctgtc cctcggtttc
    17341 cttgtggaag tggacgccct tgcggaggtg gaaggtccac accttgccgc cgtcgctgcc
    17401 ttcccaggat tcggcgaggg ccggagtgac gcccttaccg tcggcgtcgt tctccacgag
    17461 ggagtcgaac atcaggtaga ccacccgact ggaagtggtg tcggtgacct gagcggggtc
    17521 caccttgggg ggatcgttga tctcgtgcca gcggaggacg ccccccatca cgggctcatc
    17581 ggcaaaagcc acggaagcaa agagggcgct aagccccaac gccagggtca aaagaatcag
    17641 tccgtgcttc ttcattgtcc aaaccacccc ttcttgaagt ctcgatctcg gtaaaaacgc
    17701 tatcctattg cccgccaccg cccgccttga aagcccctta caaacttttc gccgccgtgg
    17761 accaccacct ccccaacaac ttggaagtcc aatttcttcc gccgccagta ctataacatc
    17821 ggggaaaggt ttcgtcacga aagctcctgg gcgttcaatt aaaatttcta ttaaaaacat
    17881 cctcgttggc ggaaataaaa gtgcgccgaa acgggatctt cggcgcactt taaagcgaca
    17941 tcaagcaaat gtaaagaatt gtatgctata ggtttttaac cttattgaaa atcgacgtgg
    18001 gggagtattg gtgggacttc cccttcttga catggtggta gtccgcgtaa gtaaggggag
    18061 acccttcccg gatcacggcg gcccccacca cttctcccac aaacatgatg tgggtatgca
    18121 cgtccagggt gctgatcacc cgggcatcca gggcggaaac ggtccaatcc atcacgtagg
    18181 gagcccccgt gggaccctct cggtgttgca cgttgcagaa cttgtccaag tttcgaccgc
    18241 acttgaagcc aaactgaccg atgaaggtca tgggcacgtc ctgccccagc
//
LOCUS       DAOOER010000037        18104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_3555_length_18104_cov_7.804421, whole genome shotgun sequence.
ACCESSION   DAOOER010000037 DAOOER010000000
VERSION     DAOOER010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 18104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18104
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3555_length_18104_cov_7.804421"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="PL162_06225"
     CDS             complement(<1..917)
                     /locus_tag="PL162_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQF91651.1"
                     /translation="MGKYIFRRILSLVPVLIGVSFIVFTLLYLTPGDPARMALGDQAT
                     EEAVQEFRNKEGLNDPFLVQFSRYIYKAAFHGDIGRSYVTKRPVTDEIMATFPATLKL
                     SALAVTIAILLGIPFGIISAIKQYSLFDSITMFFALVGLSMPVFWLGLLLILFFSVYL
                     GWLPSSGFDTFSAMILPAVALSAQVLAIVTRMTRSSMLEVVRQDYIRTARAKGQVESK
                     VICRHALGNALIPIITICGLQFGQLLGGAILTESIFSIPGVGRLMVEAIKMRDYPVVQ
                     GGVLYVAVAFSIVNLLVDLLYAFVDPRIKA"
     gene            complement(1053..2561)
                     /locus_tag="PL162_06230"
     CDS             complement(1053..2561)
                     /locus_tag="PL162_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQF91652.1"
                     /translation="MQRFGKALVWALCLSMVLAVGAFAEAKDTLVVANIYDAKTLDPV
                     ATNDVASSGICLHIYDNLLTLGPDGVPVPQLAEKYEIIDATTYKFYLRQGVTFHNGEP
                     FTAADVKYTFERAKTPLGASIRQYVDDIDTVEVVDDHTVIFKLKRPFTPFLLALTHTA
                     GSILNQKAVEAAGENYGMNPVGTGPFAFKAWAKGDKITMERYDGYWGKKPAYKTLVMR
                     SIPEATNRTIELESGAVDLAYQIVTNDLKRVEENPKLALLRVVDNSTTYMGFNCAKAP
                     FDNPKVRRAISMALDTVGIQKAVWRGVGKSPVGPIAPNVLYSYKDFQPHVQNVEEAKK
                     ILAEEKVSLPMNVQIWTNERKERIDMATIIQSQLADLGINAEIKVLEWGAYLDGLKQK
                     THDMFLLGWVASVPDPEFALSGVFHSSMVGSTNFTYFIDETVDKLMEEGKALPNGPDR
                     EKTYVELQKRIDDLCPWVYLHNDEQIVGAQKTVKGFVPSPRGYHVLTDVTFE"
     gene            complement(2875..4065)
                     /locus_tag="PL162_06235"
     CDS             complement(2875..4065)
                     /locus_tag="PL162_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQF91653.1"
                     /translation="MMERLRGAAAEIAAEMTAWRREFHAHPELSFEEVGTTRRVAELL
                     RSFGYDNLKVGLGGVPTGVVADLGVGRAGWGVALRADLDALPMEETADRPYRSQKPGV
                     MHSCGHDAHTAMLLGAAKILKCLEPEIPGRVRFIFQPSEESPHRSGARAMIAEGALEG
                     MDAIAGIHVWSGLPSGVLGYRPGAAMASSDEWECVITGRGGHGAMPHEAVDPVVAASA
                     LVGMLQTVVSRRISPLETAVVTVGKIEAGTTYNIIPEKAFLQGTVRTFNPEVRRRVPE
                     IMGRIVQGVAGASECTAEFNYRETLPPTVNDPIFTDLARRVGEDLLGGDGVRLVEPTM
                     GAEDMSLYLERLPGAFLFLGVGNEAKGTTFPQHHPAYDIDEAVLPLGCAFLSSLAWRF
                     LAGT"
     gene            complement(4159..5673)
                     /locus_tag="PL162_06240"
     CDS             complement(4159..5673)
                     /locus_tag="PL162_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQF91654.1"
                     /translation="MIRKGKIFVWVLALGLAFGLAFGGAGEARDSLTVAHLCDAKTLD
                     PIGTNDVVSSGIDLHIYDTLLTLGTQGELVPQLAESYEVIDPTTYKFSLRHGVTFHNG
                     EPFTAKDVKYTFERAKTPLGASIRQYVDDIDSVEVVDDFTVIFRLKRPFAPFLCTLTC
                     AAGSIVNQKAVEAAGDNYGMNPVGTGPFVLKSWARGDKIVLERYDGYWGTKPAYKTLV
                     VRSVPEAVNRTIELESGAADIAYYVSPSDIKRVEENPKLSLLRTMDNTTTYMGFNCAK
                     APFDNPKVRIAVSMALDTIGIQRAAWRGVGKAPIGPVAPGVRYSSKNFVPHVQDVEGA
                     KKILAEEGVALPMTAEIWTNDRKERVDMATIIQSQLEEIGIHVEIKVLEWGSYLENLK
                     RGTHDMFLLGWPAAAPDPDFALTGVFHSSMAGATNFAFFKDAEVDRRLDEGRGMEDGP
                     DREAVYEAVQQRIDALCPWVYLHNDEQIVGTRKDVHGLVLSSRGYHLLTPVTFE"
     gene            complement(5897..6592)
                     /locus_tag="PL162_06245"
     CDS             complement(5897..6592)
                     /locus_tag="PL162_06245"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQF91655.1"
                     /translation="MQVPTLTELALKKLENLILEGTLCPGDRINLEELSRTFTMSPTP
                     FREALNRLERERLVTYRPRAGWTVAQVSREEFFQSYEMQELLETTMTVRAMEKLDGDD
                     LDRLAEWNREMRLRHGRWDGLGVIEANLAFHKVIYDRYPNAIMRRTLEQVWKATHLER
                     RWVMIQDTVECVPRLMDEHDDLIKALSQRDEAAVRGVMARHFRTGEESFKVYFDRLGM
                     ETEEGEEEREGRL"
     gene            complement(6933..7676)
                     /locus_tag="PL162_06250"
     CDS             complement(6933..7676)
                     /locus_tag="PL162_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HQF91656.1"
                     /translation="MRFAVAVGSGKGGVGKSTVTALLGVGLSRRGKRVGILDADLTGP
                     SIPRLLNLTDPAMSFGDGILPARSSRWGISAISINLLLEDPENPVIWRGPLLGGVVRQ
                     FWNDVSWGELDVLLVDLPPGTSDAPLTLLQTADLDGFLGVTTPQGLAGMVVEKSLRMV
                     DRLGVPILGLVENWSSLECPFCGERTRPFGGQAVDVLGDRWGVKERIRLPLDPELMVR
                     GDRGDLEDYDNPAVMEPLLEGFFRALPGV"
     gene            complement(7816..8166)
                     /locus_tag="PL162_06255"
     CDS             complement(7816..8166)
                     /locus_tag="PL162_06255"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HQF91657.1"
                     /translation="MIVAFASSGRTLEAAPADRFARAPFFLLVEKGTLKEVVENEEAE
                     GASGVGGKVVQMLLQKGVGAVVAPKYGPKAGDALKAAGMASWSGTGATVRELAEAFKA
                     GSLSPAVEGSPHHA"
     regulatory      8590..8675
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            9518..11560
                     /locus_tag="PL162_06260"
     CDS             9518..11560
                     /locus_tag="PL162_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HQF91658.1"
                     /translation="MSMSHGDLWFQWMWIFLATAPLGALLLGQWPRASTLWASAFGAL
                     GSLAGIGASLIVLGGSGGGLRWTIDTALPFLTADICLDALAAFFLLLLSVVNLAVSIY
                     IQGYAAHYAGRRPLGVLHFLTGTFVMAMAGVFVAHSYTLFFLLWEAMSLLSFFLVVFE
                     PEERGTPQAGMFYLVMTHLGSAFLLVGLLGLMALSGGQGMTPLATPLTGPLRWGFFAL
                     FLVGFATKGGVAPLHVWLPYAHPAAPSSVSALMSGVMIKTAVYGMVRFFFDLLGPVGL
                     GEGAVLMGLGGLSALTGVAYAYTDRNIKRMLAYSSIENMGIITLFLGTGAVAIATGHP
                     GAGALGLGAALLHTLNHALFKGMLFLGAGSVHQATHTKHMDDLGGLMKTMPRTGALML
                     LGGLALASIVPMNGFIGEWLGLQSLAILVMRGGAALGIPALLGAATVCLAGALAAGAV
                     VKFLGVAFLGTPRTRPAAEAQEVPPSMWGAQAFLGGLCLLGGLFPGPFLALVRRGLAP
                     ALTTPWPSPLAGGVAFLWAPMRGELSSAWPLGAIFLGAAAVVVLTWGLWKTIRHPEIR
                     RYGTWDCGYGTLSPRMQYSARGFANPLVIVFRALFRPTRELSVVPGPSPYHPRGVRYT
                     TRTERLFESRLYLPVMDRASRLSRLARGFIQTGSIHLYLVYIFVTLLALLLYARIA"
     gene            11633..12583
                     /locus_tag="PL162_06265"
     CDS             11633..12583
                     /locus_tag="PL162_06265"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HQF91659.1"
                     /translation="MRTLMELVLHLALVLALAPLPGGVIRKIKAFVQKRQGPSLFQEY
                     RDLRKLFRKDMVISDRASWVFFAAPFVVFGSSVAAAALVPLLPWAGGAEFPGDVFLVI
                     SLLALGRFFLALGGMDPGSTFGGMGSSREMTISALLEPAFLVFTLALGLQAGAPSFGV
                     ILRHAATLGTAVWHPSFLLLLFSLILVTLGETARIPVDDPSTHLELTMVHEAMVLEYS
                     GPFLALMEYGAAVKQLLFLTVLAQVFLPFGNAALAGMGIGGVVLGLPLAAAKVLVMAA
                     VAGVAEVFTVKLRIFSVPNLAAMAFIAAFLGFLNLMVFGG"
     gene            12588..13238
                     /locus_tag="PL162_06270"
     CDS             12588..13238
                     /locus_tag="PL162_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91660.1"
                     /translation="MDGTHFLSVAILLSTFALVANKRVRSYIRTFQVQSALVALGALV
                     VGTEHFSDRGEWDLLVVAAITLGVKVWAIPRVLLKAHGQGEPRREKDFFYNIPLLVLC
                     CCALTAFAYFAVLGNGVPADRGASHLINAVAVVLMGLFFMISRRFALGQIVGFLVMEN
                     GLFVASLFAAGGMPFVVDLGVFVDVLSAVLIMGGLVFQLSEKFHSLDMGKLRKLRG"
     gene            13243..>14034
                     /locus_tag="PL162_06275"
     CDS             13243..>14034
                     /locus_tag="PL162_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HQF91661.1"
                     /translation="MMFVLLGTPLAVIPLFLLARHALLLFLVGTGGSLAMLVAGGVLG
                     HTISATGPVAWGAPWYLYVDSLGFLTLGLVLLVSVAVNVFSWGYFQEELGRGHTELRG
                     LRLFYSLLFAFIFSMVVALTTENLGILWIAIEGTTLASAFLVGFENEKRSLEAAWKYM
                     ILCTVGIALAFLGITILHMASGGTDGGAGTALSWPFLMAHADKLDGALLRLAFVFALV
                     GFGTKAGLAPLHNWLPDAHSQAPSPVSAMLSGVLLNSAMYGILRVT"
     assembly_gap    14035..14134
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14135..14478
                     /locus_tag="PL162_06280"
     CDS             <14135..14478
                     /locus_tag="PL162_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91662.1"
                     /translation="LFLGFLAIGGLPPFSVFFSELGLLTSCFQGGYLGLGAGMTLLLA
                     LIFVGFAVTLHRVFVGDLPADFSVEPGEPNAPGLVVMILLLVVIGVSGFALPSEAWSL
                     LRDATRIVMGG"
     gene            14483..16063
                     /locus_tag="PL162_06285"
     CDS             14483..16063
                     /locus_tag="PL162_06285"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HQF91663.1"
                     /translation="MALTSKAALQEALGRWGGTVTPRGNELRLDATVETLRSLGIFLQ
                     DYLGLSLAMLFATDERSLECGGFVLRHVFAAPEAGAFVVVRTPLPPGEERFPSLGDRV
                     PSASLYEREISDLFGLIPEGHPQLKPLVFHSHWPEGVHPLRRDYDRRHRPPYVSREIP
                     FLEVEGSGVYEIPVGPVHAGIIEPGHFRFSVAGEPVINLEAQLFFVHRGIEKLCEGMA
                     AEKALFVAERVSGDESFANSLAFCQAVESLASVEIPPRAAVGRVIFAELERLTSHLGD
                     LAGICLDTAYGFGAFQFRMLRGWAYLMADLLCGMRFLRSVNAPGGLRRDFFGDQIPEA
                     VKLAERIRDELEDTAAMVRRNGFFIDRVENTGILSRDIARDLHATGPGGRASGVPHDV
                     RRSYPYAAYPAMAFDVPCYPHGDVACRMNVKLDEARQSVLMIIQGLGALPPGEIRETL
                     PPLPPFHSALGCVEAPRGESLHWILTGEDDTLFRYKIRTPSFCLWPALCHAVRGNVVP
                     DFPLINKSFNLSYAGNDL"
     assembly_gap    16174..16273
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16274..17072
                     /gene="nuoB"
                     /locus_tag="PL162_06290"
     CDS             <16274..17072
                     /gene="nuoB"
                     /locus_tag="PL162_06290"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HQF91664.1"
                     /translation="TLGYPAVPMDDRRFVGCPAIDARRCTRCGACEAACPSSCMGRTA
                     EGIVGINLDMCLFCGRCAEVCPTGAATMTRRFELATTSRENLRVTPLSPAPPAPRGEE
                     EPLVSEMRRRIDHAYGRSLRIREVDAGSCNGCDYEVNALNNPFNDLERFGIQFVASPR
                     HADMLLVTGCPTRNMEQPLIKTFNATPDPKLVVAVGACACSGGIFRDLYATENGIDRV
                     IPVDVYVPGCPPRPQAILHALFKALEGVRPMPRGQGSPDGEGISHEP"
     gene            17112..>18104
                     /locus_tag="PL162_06295"
     CDS             17112..>18104
                     /locus_tag="PL162_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91665.1"
                     /translation="MTAPLRSKPASIASLEALLDPVRDDLARRAAAAALIPLGPGPYP
                     DEVALEASRSAAELTLRQGFFEALASRHERPSCTSEEIPPDGRARGLSPRRLAQGAAL
                     PPLLLPPPEDLSSPSPSRVAFAAAAGALGGAALGGTLAPWAFLSPQAGLVAGIFLGAW
                     TAARGMGALDEASGVRRLLALALGVATLGEGWGLMTERRSSVGLWHLLEGRSTGLRRL
                     SFFVALTVLFFLTRRPRRFSPAAYERVAKNCALQWLEGALRGVAFAENDFTLREMESR
                     KNLPTAEERALLALAEEVRSWDPSSLESIPQAWEDLHREMESQGISGEKDLPTHW"
BASE COUNT         2904 a         5915 c         5523 g         3562 t
ORIGIN      
        1 gccttgatcc gcgggtccac gaaggcgtag agcaggtcga ccagcaggtt cacgatgctg
       61 aaagccacgg cgacgtagag cacgcccccc tgcaccaccg gataatcccg catcttgatg
      121 gcctccacca tgaggcgccc cactccgggg atggagaaga tggattccgt aagaatggcg
      181 ccccccagga gctggccgaa ttgaaggcca cagatggtga tgatggggat gagggcgttt
      241 cccagggcgt ggcggcagat gactttggat tccacctgac ccttggcccg ggcggtgcgg
      301 atgtagtcct ggcggaccac ctccagcatg ctggaccggg tcatgcgggt gacgatggcg
      361 agcacctggg ccgaaagggc caccgctggc aaaatcatgg cggaaaaggt gtcaaagccc
      421 gacgacggga gccacccgag gtacaccgaa aagaaaagga tcaacaaaag ccccagccag
      481 aagaccggca tggaaagccc cactagggca aagaacatgg tgatggaatc gaaaagcgag
      541 tactgcttga tggccgagat gatgccaaag gggatcccca gcaatatggc aatggtcacc
      601 gctagcgccg aaagcttcag cgtggcggga aaggtggcca tgatctcgtc cgtcacgggg
      661 cgcttggtga cataggaccg gccgatgtcc ccgtgaaacg ccgccttgta gatgtagcgc
      721 gaaaactgca ccagaaaggg gtcgttcaat ccctctttgt ttcggaactc ctggaccgcc
      781 tcttccgtgg cttggtctcc cagagccatg cgggcggggt cccccggcgt gaggtacaaa
      841 agggtgaaga cgatgaatga cacgccgatg agcaccggaa cgagggagag aattctccta
      901 aaaatgtact ttcccatgct ttcccttcct ttcccggcac ggaaaacggg agcacggacc
      961 gcaagcccac gctcccgtca agggtagtct ttccctccgg cggagcgccc catgggaacc
     1021 ccgccggagg gaaggccacc gaagcggcgg tcttactcga acgtcacatc ggtgaggacg
     1081 tgatatcctc gagggctggg gacgaagcct ttgacggtct tttgggcgcc cacgatctgc
     1141 tcgtcgttgt gcaaatagac ccaggggcac aggtcgtcga tgcgcttctg gagctccacg
     1201 taggtctttt cccggtcggg gccgttgggc agggccttac cctcttccat cagcttgtcc
     1261 acggtctcgt cgatgaaata ggtgaagttc gtggatccca ccatggagga gtggaacacg
     1321 ccggagaggg cgaattccgg atcgggcacc gacgccaccc atcccaggag gaacatgtcg
     1381 tgggtcttct gcttaaggcc gtccaggtag gccccccact ccagcacctt gatctcggcg
     1441 ttgatgccga ggtccgccag ctggctctgg atgatggtgg ccatgtcgat gcgctccttg
     1501 cgctcgttcg tccagatctg gacgttcatg ggcagggaga ccttctcctc cgccaggatc
     1561 tttttggcct cttccacgtt ctgcacgtgg ggctggaagt ccttatagga gtaaagcacg
     1621 ttgggagcga tgggtcccac gggggacttg cccacgccgc gccacaccgc cttctggatt
     1681 cccaccgtgt ccagggccat gctgatggcc cggcgaacct tggggttgtc gaagggagcc
     1741 ttggcacagt tgaagcccat gtaggtggtg gagttgtcga ccacccggag cagggcgagt
     1801 ttgggatttt cttccacgcg cttcaggtcg ttggtgacga tctggtaggc caggtccacc
     1861 gcgccgctct ccagctcgat ggtgcggttc gtggcttcag ggatggagcg catcaccagg
     1921 gtcttgtagg cgggcttttt cccccagtat ccgtcatagc gctccatggt gatcttgtcg
     1981 cccttggccc aggccttaaa ggcgaagggg ccggtcccca cggggttcat gccgtagttc
     2041 tcccccgccg cttccacggc cttctggttg aggatgctcc ccgccgtgtg cgtcagagcc
     2101 agcaagaagg gggtaaaggg gcgcttcagc ttgaagatca ccgtgtgatc gtccaccact
     2161 tcgacggtgt cgatgtcgtc cacgtactgc cggatggagg cccccagggg ggttttggcg
     2221 cgctcgaagg tgtacttcac atccgctgcg gtgaagggct cgccgttgtg gaacgtcacg
     2281 ccctggcgca gatagaactt gtaggtggtg gcgtcaatga tttcgtactt ctccgccagt
     2341 tggggcaccg gcacaccatc ggggcctagg gtgagcaggt tgtcgtagat gtgcaggcag
     2401 atccccgagg atgccacgtc gttggtggca acggggtcca aggtcttggc atcgtagata
     2461 ttagccacca ccagcgtgtc tttggcctcc gcaaaagccc ccaccgccag caccatcgac
     2521 agacacaacg cccaaaccaa cgcctttcca aacctttgca tgcctatccc ctctttctat
     2581 cgctatcgac aagtagcttc cctggcgccg ctccggtcct ttacgccctt ggcgacggag
     2641 gcccatgcct ccatccgaag cgcccgcccc gcgccaccca ccccagggat catggggaaa
     2701 tctagcgtct agcccctgac cccaagagcc ctctttccta aaacccctcc gctggcgacg
     2761 tcgggagagc ccgcctcgcg ccgcctccgg tgccgccctg cccccatccc ctccggcctc
     2821 gccatacccc ctttccaggt ctcttttgca aaaacccttc ccctagggca acccctaggt
     2881 tccggcaaga aagcgccacg ccaggctcga cagaaaggcg catcccaagg ggagtacggc
     2941 ctcgtcgatg tcgtaggccg ggtggtgctg gggaaaggtg gtacccttgg cttcatttcc
     3001 cacccccagg aagagaaagg cccccggaag gcgctccagg tagaggctca tgtcctccgc
     3061 ccccatggtg ggctccacca ggcgcacccc atcgcccccc aacaggtctt cccccacgcg
     3121 cctcgccagg tcggtgaaga tgggatcatt aacggtgggg ggaagggtct ccctgtagtt
     3181 aaactccgcc gtacactccg aagctcccgc caccccttgg acgatgcgcc ccatgatttc
     3241 cggcacacgg cgccggacct ccgggttgaa ggtgcgcacg gtgccctgca aaaaagcctt
     3301 ttcggggatg atgttgtagg tggtgcccgc ctcgatcttt cccaccgtga ccacggcggt
     3361 ctccagggga gaaatccggc ggctcaccac cgtctggagc atgcccacca gggccgaggc
     3421 cgccaccacg gggtctacgg cctcgtgggg catggcgccg tgtcctcctc gccccgtgat
     3481 gacgcactcc cactcatcgg aagaagccat ggcggcaccg ggacgataac ccaatacccc
     3541 cgaagggagg cccgaccaga catggatccc cgcgatggcg tccatcccct ccagggcacc
     3601 ctcggcgatc atggcccggg ccccgctacg gtgaggggat tcttcggagg gttgaaatat
     3661 gaaacgcacc cgtcccggga tctccggttc caggcattta aggatcttgg cagcccccaa
     3721 cagcatggcg gtgtgggcgt cgtggccgca agaatgcatc actccgggct tttgggaccg
     3781 gtaagggcga tcggcggtct cttccatggg gagggcgtcc aggtccgccc gcagggccac
     3841 gccccacccc gcccttccaa cccccaggtc ggccaccacc cccgtgggaa ctccccccag
     3901 ccccaccttc aggttgtcgt acccaaagga cctcaggagc tccgccaccc gacgggtggt
     3961 ccccacttcc tcaaacgaga gctcgggatg ggcgtgaaac tcccgacgcc acgccgtcat
     4021 ctctgcggcg atctccgccg ccgccccccg aaggcgctcc atcatgatct attcatcccc
     4081 tctctccacc aaggccggaa agaccgccga aggcccatat caaggccttt tgaagcatcc
     4141 tccgccaccg cataaggcct attcgaaagt caccggagtc aggagatggt atccccgaga
     4201 gctcaggacc aggccgtgca cgtccttccg agtgcccacg atctgttcgt cgttatggag
     4261 gtagacccag gggcagaggg catcgatccg ctgttgaacc gcctcgtaga cggcctctcg
     4321 atcgggcccg tcttccatgc cccttccttc gtcgagcctt cgatccacct cggcgtcctt
     4381 gaagaacgcg aagttggtgg cccccgccat ggaggagtga aacacccccg tcagggcgaa
     4441 atccggatcg ggggccgccg ccggccaacc cagcaaaaac atgtcatggg tgccccgttt
     4501 cagattttcc aggtaagacc cccattccag cacctttatc tccacgtgga tgccaatttc
     4561 ttccagctgg ctctgaatga tggtggccat gtccacgcgc tccttgcggt cgttggtcca
     4621 aatctccgcc gtcatcggca gcgccacgcc ctcttccgcc aggatctttt tggccccctc
     4681 cacgtcctga acatggggga caaagttctt ggaggagtag cgcacccccg gagccaccgg
     4741 tccgatgggg gctttcccca cgccgcgcca ggcggccctc tggatgccga tggtatcgag
     4801 ggccatgctc acagcgatcc gaaccttggg gttgtcgaag ggggccttgg cgcagttgaa
     4861 ccccatgtag gtggtggtgt tatccatggt ccggagcagg gaaagcttgg gattttcctc
     4921 cactcgcttg atgtcgctgg gagaaacgta ataggcgatg tccgccgcac cgctttcgag
     4981 ctcgatggtc cggttcaccg cctcgggaac ggagcgtacc accagggtct tgtaggcggg
     5041 ttttgtcccc caatatccgt cgtagcgctc caggacgatc ttgtcccccc gggcccaact
     5101 tttcagcaca aagggccccg ttcccacggg gttcatgccg tagttgtccc cggcggcctc
     5161 cacggccttc tggttcacga tgctccccgc agcgcaggtg agggtgcaca aaaaaggcgc
     5221 gaaggggcgt ttcagccgga aaatcacggt gaaatcatcc accacttcga cggagtcgat
     5281 gtcgtccacg tactgccgga tggaggcccc caggggggtc ttggcgcgct cgaaggtgta
     5341 cttgacgtct ttcgccgtga agggctcgcc gttatgaaaa gtcaccccgt gccgcaggga
     5401 gaacttgtag gtggtgggat cgatgacctc gtagctttcc gccagctggg gcaccagctc
     5461 tccttgggtt cccagggtga gcagggtgtc gtagatgtgc aaatcgatcc ccgaagaaac
     5521 cacgtcgttc gtcccgatgg ggtccagagt cttggcgtca cagaggtggg ccaccgtgag
     5581 cgagtcccgg gcctcccccg cccccccgaa cgccaacccg aacgccaacc ccaacgccaa
     5641 aacccacacg aaaatctttc ccttccgtat caccgagctt cgcccccttc gttctgaaaa
     5701 ccgccgcctt ttccctcccc ggagatctcc cccagggccc gtccgcccct caaggcgcgt
     5761 cgaaggccat gaccggctcc ttgaggatcc tcttttcggc cgctgccagg acttttatcc
     5821 catcccggag cgcctttcgt cgtgccaaaa cccgggggat cggttcggcc cagtcccccg
     5881 cgccgaaggg cttttgtcag aggcgcccct ctctttcctc ctctccttct tcggtctcca
     5941 tgccgagtct gtcgaaatag accttgaagg actcttcccc cgtgcgaaag tgccgagcca
     6001 tcacacccct caccgccgcc tcgtccctct gggagagggc ctttatcagg tcgtcatgtt
     6061 catccatcag gcgcggcaca cactccaccg tatcctggat catcacccaa cggcgctcca
     6121 gatgggtagc cttccaaact tgttccaggg tgcgccgcat gatggcgttg gggtagcggt
     6181 cgtagatgac tttgtgaaac gccaggtttg cttcgatgac tcccagcccg tcccaccgcc
     6241 cgtggcggag gcgcatctcc cggttccatt ccgccagacg gtccaaatcg tctccgtcca
     6301 acttttccat ggcccgcacg gtcatggtcg tttccaagag ttcctgcatc tcgtagctct
     6361 gaaaaaactc ttcccgggaa acctgggcca ccgtccaccc cgcccgaggg cggtaggtca
     6421 ccagacgctc gcgctcgagg cggttcaacg cctctcgaaa gggcgtgggg ctcatggtga
     6481 aggtccggga aagctcctcc aagttaatgc gatctcccgg gcagagggtc ccctcgagga
     6541 tgaggttttc cagctttttc aaggccaatt ccgtcagcgt cggcacttgc atggcgcacc
     6601 ctcctccggg ccatcgtcca tatattctat aggatatagg atatagcccc gatgccccat
     6661 actaaggcgt tccccggctt tctgtcaaga cctctcgggg gatttttgtt caccttagcc
     6721 ctttgtcttt acccctcatc gagcatcggg ttcgatgaac ccaatccggg ggcaaaaaaa
     6781 aacccggcgg gggtgccacg ccaaagggtg gacctcctcc ggggagcaaa aaaacccgtc
     6841 gcctcgcaga gcggctccgg gcgaataccc ccgccttccc gtgcgggata gaatcaccgg
     6901 ggcccccatc gggggcccct tctcccttcg tcctacacgc cgggaagggc ccggaaaaac
     6961 ccctccagga ggggctccat cacggcggga ttgtcgtagt cttccagatc gccccgatcc
     7021 cctcggacca tgagctccgg atccaaggga aggcgaattc gctccttgac tccccatcga
     7081 tcccccaaga cgtccaccgc ctggccccca aagggtctgg tcctctcgcc acagaagggg
     7141 cactcgaggg agctccaatt ttccaccagc cccaggatgg gcactcccag ccgatccacc
     7201 atgcggaggg atttttccac caccatgccc gccaggccct ggggagtggt caccccgagg
     7261 aagccatcga gatcggcggt ctgaagcagg gtgaggggag catcggaagt gccgggggga
     7321 aggtccacca aaagcacgtc gagctcgccc caggagacgt cgttccagaa ttggcgtacc
     7381 acgcccccca gcagggggcc ccgccagatc acgggatttt cgggatcctc cagcagcaga
     7441 ttgatggaga tcgccgagat gccccaccgg gaggaacggg ccggaaggat gccgtcgccg
     7501 aaggacatgg ctggatcggt gaggttcaga agtcggggaa tggaagggcc cgtgagatcg
     7561 gcgtctagga tccccacccg cttgccccgg cgagacaaac ccacccccag cagggcggtg
     7621 acggtgcttt ttcccacgcc tcccttgccg ctccccaccg ccacggcaaa gcgcaccccc
     7681 cggggtttgg tacgcggggg aagggcgtcc tcctcctgaa tgggggattc accggaggga
     7741 caagacgggg ccgaggggca agaggggcag gaagatgacg ggtaagaggg ggccgggccc
     7801 cccttcgggg agagctcagg catgatgggg gctcccttcc accgccgggg ataggctccc
     7861 cgctttgaag gcttccgcca gctcccgcac cgtggctccc gttcccgacc acgacgccat
     7921 gcccgccgcc ttcagggcgt cccccgcctt gggaccgtac ttgggggcca ccaccgctcc
     7981 cacgcctttt tggaggagca tctgcaccac cttgcccccc acgccggagg ccccttcggc
     8041 ctcctcgttt tccaccactt ccttcaaggt ccccttttcc accagcagga aaaagggagc
     8101 ccgggcaaag cggtccgccg gagcggcctc caaagtcctt cccgaagatg caaacgccac
     8161 gatcatgacc cgcgcctcct tatgatatcg attctcattt agagtacccc ccctgaggcc
     8221 cttcgtcaag gggaagaatc ccttcgcccc ccctttcggc agtgggggaa ctgtccgtcg
     8281 ttcaccaaaa aaccgatgat catcccttct cccgtcttgc gccccggggg ccccggtcgt
     8341 tccaaaacgc cttacggcgc ccccgcgcca ccatgcgaga gcccgggggg cgcctttgac
     8401 ccgggccgcc tttctccgga ctttcgcgat gacgcttcgg ccttgagcgg ataataaaga
     8461 gaacttcgac gggcattttt tcgcctaaac ttgatcgcgt caaagagaaa ccatagcaaa
     8521 gaaagacccc ttgacaagga acttttttta tctatacttc gacaaggtgg ggtggtatgg
     8581 atcgcccccc tgcgccaagg gcaaagccga ccgaaaggcg gcgacgcaaa gccacgggtc
     8641 taaagccgaa gggccatgac agccgggttg ccgcagggaa aagcgcgagc gtctgcgcgg
     8701 ccttttcttt gtggcgtctg tccacgggaa ggggccgcgc tcccttttgc ccggccctcc
     8761 ccccgagtga gagaggagga cggcatgaga cgcttgttca caggggcggt atttatggga
     8821 gttttggcca ctgccatgat ggcgggaagg gcctgggggg tcgcgctctc ggcctgggcg
     8881 cgcctcgaag ggctctagtg gggcttcgtg caaagctcat ggacctcgcc gtaatccctt
     8941 tcctcccgag gtccggtctc cggggcccca gagatcttcc ctccccggtc gtttttcgcc
     9001 ccctccttgg ccgaactcga ccggggaggg ggcgaaaacg gcttcggcca ccctccacgc
     9061 catcgtcgag atgccttctt ttcgtcaccg atgccaccca aaacttttcc cctcgatgct
     9121 ccttttttcg gagcttccag acaaatagga cttaagaccc ataaacatat ctccccccgg
     9181 ggaggacacc cttcgatcct cgtggtatct ttaggtggcc ggaaggaccg gggcgacttt
     9241 ggctcgtcaa gggggctcct tcctggcttt tggcgaagct gaaccagacg ccctcccggg
     9301 ggggcgtgtc gtttccgggg caggggcccc ggggggtcag gcggtcccgg gggctttcgc
     9361 ttcctcgggc ctccctgtgg cccgggcgat ggggaccgag ggggccccgg tgccctcgac
     9421 tccggggcgt caggatgggg ttttcccctg cgccggaggg gcccaaccct cctcccgaat
     9481 tacctgcacg aagaagcacg aaaaggagga tttccccatg tcgatgagcc atggagacct
     9541 gtggtttcag tggatgtgga tctttcttgc caccgccccc ttgggggccc ttcttttagg
     9601 ccagtggccc cgggccagca ccctctgggc atcggccttc ggcgccctgg gatccctggc
     9661 ggggatcgga gcctccctca tcgtcctggg gggatcgggg gggggcctgc gatggaccat
     9721 cgacacggcg ctccctttcc tgacggcgga tatttgtctc gatgccctgg cggcgttttt
     9781 cctgctcctc ctttcggtgg tcaatttggc ggtgtcgatc tacatccagg gatacgcggc
     9841 ccattacgcg gggcgaagac ccctgggggt gctccacttc ctcacgggga cctttgtgat
     9901 ggccatggcg ggggtcttcg tggcccactc ttacaccctc tttttcctcc tctgggaagc
     9961 catgtctttg ctctctttct tcctggtggt ttttgagccg gaggaaaggg gcactcccca
    10021 ggcagggatg ttctacctcg tcatgaccca cctggggtcg gcctttctcc tggtgggact
    10081 cctggggctc atggcccttt cgggggggca gggcatgacg cccctggcca cccccttgac
    10141 gggccccctg cgctggggct ttttcgccct tttcctggtg ggtttcgcca ccaagggggg
    10201 ggtggcgccg ctccacgtgt ggcttcccta cgcccatccc gcagccccca gctcggtgtc
    10261 ggccctcatg tcgggggtca tgatcaagac ggcggtctac ggcatggtgc gcttcttctt
    10321 cgacctcctg ggtcccgtgg ggctggggga gggggccgtc ctcatgggac tgggggggct
    10381 ttccgccctc accggggtgg cctatgccta caccgatcgg aacatcaaac gcatgttggc
    10441 ctacagcagt atcgaaaata tggggatcat cacccttttt ctcggcaccg gagccgtggc
    10501 catcgccacg ggccatcccg gggcgggggc cctgggactg ggagcggccc tgcttcacac
    10561 gctgaatcac gcccttttca agggaatgct cttcctgggg gcggggtcgg tgcaccaggc
    10621 cacccacacc aagcacatgg acgacctggg ggggctcatg aagaccatgc ctcgcacggg
    10681 ggccctcatg ctcttgggag gattggccct ggcctccatc gtccccatga atggcttcat
    10741 cggagagtgg ttggggcttc aatcactggc cattctggtg atgcgaggag gggccgcgct
    10801 gggcattccc gccctgttgg gggcggcgac ggtctgtctg gcgggagccc tggcggccgg
    10861 agccgtggtc aagtttctcg gggtggcctt tttggggacc ccccgcacgc gccccgcagc
    10921 ggaggcccag gaggtgcctc cctccatgtg gggagcccag gcctttttag gggggctgtg
    10981 cctgctggga gggctttttc ctggtccctt tctcgccctg gtccgccgag ggctcgcccc
    11041 ggccctgacc accccttggc ccagccctct ggcggggggc gtggccttcc tctgggcccc
    11101 catgagggga gagctttcgt cggcttggcc cctgggggcg atctttctgg gggcggcggc
    11161 ggtggtcgtc ctgacttggg gcctgtggaa gacgatacgc catccggaga tccgccggta
    11221 cggcacctgg gactgcgggt acggcaccct cagccctcgc atgcagtact ccgcccgggg
    11281 gttcgccaac cccttggtca tcgtctttcg cgccctcttt cgtcccacca gggagctctc
    11341 cgtcgtaccg gggccttccc cctatcaccc ccggggggtg cgatacacga cgcgcacgga
    11401 gcgcctcttc gagagccgcc tctacctccc ggtgatggac cgggcctccc gcctctctcg
    11461 cctggcccgg gggtttatcc aaaccgggag cattcacctg tatttggtgt atatttttgt
    11521 caccctgctg gcgctcctcc tctacgcccg catcgcctag cgcccgccat cgtatgtggc
    11581 cccgccatcg cctttcaagg gacttcgcgc gcacgccgga aggagacttc gcatgcgaac
    11641 gttgatggag ttagtgttac atctggccct cgttctcgcc ctggcccccc tccccggggg
    11701 ggtgatccgc aagatcaagg ccttcgtcca aaagcgtcaa gggccgtccc tgttccagga
    11761 gtaccgagat ctccgaaagc tctttcgcaa ggacatggtg atttcggatc gagcctcctg
    11821 ggtctttttc gccgccccct tcgtggtctt cggatcgtcg gtggcggcgg cggccctggt
    11881 gcctctgttg ccctgggccg ggggagcgga attccccggg gacgtgtttc tggtcatttc
    11941 ccttttggcc ctggggaggt ttttcctggc cctggggggg atggaccctg ggagcacctt
    12001 cggaggcatg ggatcgagtc gggagatgac gatctccgcc ctgctggaac ccgcgttcct
    12061 cgtcttcacc ctggcactgg ggctccaggc cggggcaccc tcttttgggg tgatcctgcg
    12121 ccacgccgca accctgggga cggcggtgtg gcacccttcg ttcttgctgc tccttttttc
    12181 cctcatcctg gtgacgttgg gagaaacggc ccgcattccc gtggatgacc cctccaccca
    12241 tctggagctc accatggtcc acgaggccat ggtactggag tactcggggc cttttctcgc
    12301 cctgatggag tacggcgcgg cggtgaaaca gctcttgttt ctcacggtgc tagcccaggt
    12361 ctttctcccc ttcggcaacg ccgccctggc gggtatgggg atcggagggg tggtgctggg
    12421 cctgccgctg gccgccgcca aggtgctggt catggcggcg gtggcagggg tggcggaggt
    12481 cttcaccgtc aagctccgga tttttagcgt gcccaatctg gcggccatgg ccttcatcgc
    12541 cgccttcctg gggtttctaa atttgatggt cttcgggggg tgacacgatg gacggaacgc
    12601 atttcttgtc ggtggccatt ctgctgtcca ccttcgccct ggtggcaaat aagcgggtgc
    12661 ggtcctacat ccgcaccttc caggtccagt ccgccctggt ggctctgggg gccctcgtcg
    12721 tggggacgga gcacttttcc gaccggggag aatgggatct tttggtggtg gctgccatca
    12781 ccctgggggt aaaggtctgg gccattcccc gggtgctgct caaggcccac ggccaggggg
    12841 aacctcggcg ggaaaaggac tttttctaca acatccccct tttggtgctg tgctgttgcg
    12901 ccctcaccgc cttcgcctac ttcgcggtgc tcggcaacgg agttcccgcc gaccgagggg
    12961 cctcgcatct gataaacgcc gtggccgtgg tgcttatggg tctatttttc atgatcagtc
    13021 gtcgtttcgc cctggggcag atcgtgggat ttttggtgat ggaaaacggc ctcttcgtgg
    13081 cctcgctctt tgccgccggg ggtatgccct tcgtggtaga tctgggggtc ttcgtggacg
    13141 tgctgtcggc ggtgctgatc atgggagggc tggtgttcca gcttagcgag aagttccact
    13201 ccctggacat gggcaagttg cgaaagctga ggggataggg ccatgatgtt cgtccttctg
    13261 ggaacgcctc tggcggtgat tcccctgttc ctcctggccc gccacgccct cttgctcttc
    13321 ctggtcggca cgggggggtc gttggccatg ctcgtcgcgg gaggagtatt gggacacacc
    13381 atcagcgcca cgggccccgt ggcctgggga gccccgtggt acctctacgt ggatagcctg
    13441 ggatttttga ctctggggct ggtgcttttg gtctccgtgg cggtgaacgt cttctcctgg
    13501 gggtacttcc aagaggagct cggtcggggt cacacggagc tgcgggggct tcggctcttc
    13561 tactccctgc tttttgcctt tatcttctcc atggtggtgg ccctcaccac ggaaaacctg
    13621 ggcattctct ggatcgccat cgagggaacc acgctggcgt cggcctttct ggtgggcttc
    13681 gagaacgaga aacgatctct ggaggcggcg tggaagtaca tgatcctctg caccgtgggc
    13741 atcgccctgg cctttttggg tattaccatc ctgcacatgg cttcgggggg caccgacgga
    13801 ggggcgggca cggccctctc ctggcccttc ctcatggccc acgcagataa gctggacggg
    13861 gccctgctcc gcctggcgtt cgtcttcgcc ctggtggggt tcgggacgaa ggccgggctg
    13921 gcgccgctgc acaactggct tcccgatgcc catagccagg ccccttcccc cgtgagtgcc
    13981 atgctatcgg gggtattgct caacagcgcc atgtacggca tcctccgggt gacgnnnnnn
    14041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncgctct ttctgggctt tctcgccatc
    14161 ggaggccttc ccccgttcag cgttttcttc agcgagctgg ggctcctcac ctcctgcttt
    14221 caggggggat acctcgggtt gggggcgggg atgacccttc tcctggcgtt gatcttcgtg
    14281 ggattcgccg tgaccctcca ccgggtcttc gtgggagacc ttcccgccga tttttccgtg
    14341 gagccggggg aacccaacgc gccgggattg gtcgttatga tccttctcct ggtggtcatc
    14401 ggcgtttcgg ggtttgccct gccctcggag gcgtggtccc tgttgcgcga cgccacccgg
    14461 atcgtgatgg ggggataggg atatggcact cacatctaag gcggccctac aggaggccct
    14521 gggacgatgg ggggggacgg tgaccccccg ggggaatgag ttgcgcctcg acgccaccgt
    14581 cgaaaccctg cggtccctgg ggatctttct ccaggactac ctgggccttt ccctggcgat
    14641 gctctttgcc acggacgaga gatccttgga atgcgggggc ttcgtgttgc gacacgtctt
    14701 tgccgccccg gaggccgggg ccttcgtggt ggtgcgcact cccttgcccc cgggggagga
    14761 acgcttccct tccctgggag accgcgtgcc ctccgcctcg ctctacgagc gggagatctc
    14821 cgacctcttc ggcctgatcc ccgaggggca tccccagctt aaacccctgg tgttccacag
    14881 ccattggccc gaaggggtcc atcccctgcg ccgggactac gaccgccgcc acagaccccc
    14941 ttacgtatcc cgggaaatcc cctttctgga agtggagggg tcgggggtct acgaaattcc
    15001 cgtgggtcca gttcacgctg gaatcatcga gcccgggcat ttccgcttca gcgtggcggg
    15061 ggagccggtg atcaacttgg aggctcagct tttcttcgtg cataggggca tagaaaagct
    15121 ctgcgagggg atggcggcgg aaaaggctct cttcgtggcg gaacgcgttt cgggggacga
    15181 gagcttcgcc aactccctgg ccttttgcca ggccgtggaa tccctcgcct cggtggaaat
    15241 cccccctcgg gccgccgtgg gacgggtgat cttcgccgag ctggagcgcc tcaccagcca
    15301 cctgggagac ctggcgggca tctgcctgga cacggcctat ggctttggag ccttccagtt
    15361 ccgcatgctt cgggggtggg cctacctgat ggccgacttg ctctgcggca tgcgattcct
    15421 ccgaagcgtc aacgccccgg gagggctacg acgggacttc ttcggcgatc agatccccga
    15481 ggccgtgaag ctcgccgaac ggatccggga tgagctggag gacacggcgg ccatggtgag
    15541 gcgcaacgga tttttcatag atcgagtgga aaacaccggc atcctttccc gggacatcgc
    15601 ccgggacctg cacgccacgg gccccggggg acgagcctcc ggggtgcccc acgacgtccg
    15661 acggtcctac ccctacgcag cctaccccgc catggccttc gacgtaccct gctatccgca
    15721 cggcgacgtg gcctgtcgca tgaacgtgaa gctcgacgaa gcccggcagt ccgtcctcat
    15781 gattatccag ggcctggggg ccctgccccc gggagagatc cgggagaccc tgcccccgct
    15841 gccccctttc cactccgcct taggatgcgt cgaggcccct cggggggaga gtctgcactg
    15901 gatcctcacc ggggaggacg acaccctctt tcgatacaaa atccgcactc cctctttttg
    15961 cctctggccc gccctgtgtc atgcggtgag gggaaatgtg gtgcccgatt tccccctcat
    16021 aaacaaaagc tttaacctat cctacgcggg caacgacttg taaggggggc ttttcggatg
    16081 ttttccatgc tgcgcaagat gggagcctat ccccgcctca ccctgggata tcccgccgtc
    16141 cccatggacg accgccgctt cgtggggtgc cccnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16261 nnnnnnnnnn nnncaccctg ggatatcccg ccgtccccat ggacgaccgc cgcttcgtgg
    16321 ggtgccccgc catcgacgcc cgccggtgca cccggtgcgg cgcctgcgag gcggcgtgcc
    16381 cctcctcctg catggggcgt accgccgaag ggatcgtggg catcaacttg gacatgtgcc
    16441 tcttttgcgg gcggtgtgcc gaggtatgcc ccacgggggc cgccaccatg acgcgacgtt
    16501 tcgagctggc caccacttcc cgggaaaacc tgagggttac ccctctgtcc ccggcccctc
    16561 ccgctccccg gggagaggaa gaacccctgg tctccgagat gaggcggcgc atagaccacg
    16621 cctacggccg aagcctgcgc atccgggagg tggatgcggg atcctgcaac ggctgcgact
    16681 acgaggtcaa cgccctaaac aaccccttta acgacctgga gcgcttcggc attcagttcg
    16741 tggcctcccc ccgccacgcg gatatgctcc tggtgacggg gtgtcccacc cggaacatgg
    16801 aacaacccct gataaagact tttaacgcca cgccagatcc caagctggtc gtggccgtag
    16861 gggcctgcgc ctgcagcgga gggatcttcc gggatctcta tgccacggaa aacggcatcg
    16921 accgcgtgat tcccgtggat gtctacgttc ccgggtgtcc tccccgcccc caggccattt
    16981 tacacgccct gttcaaggcc ttggaagggg tgcgcccgat gccccggggg caaggatccc
    17041 ccgacgggga agggatctcc catgagccct gatcttaccc cttcctcccc ccggaagcgg
    17101 aggacggcgg catgaccgcc ccccttcgat ctaaaccggc ctccatcgct tctctggagg
    17161 ccctcttgga tcccgtgcgg gacgatctgg cccgccgagc cgctgcggcg gccctgattc
    17221 ccctgggtcc cggcccctat cccgacgaag tggccctgga agcctcccgc agcgcggcgg
    17281 aactgaccct gcggcagggg ttcttcgagg ccttggcttc tcggcacgag cgcccctcct
    17341 gcacctcgga agagatccct ccggacgggc gtgcccgggg actctccccc cgtcgcctgg
    17401 cccagggggc cgccctccct cctctgctcc tacctccccc ggaagatctt tcctccccct
    17461 ccccctctcg cgtcgccttc gccgccgcag cgggggccct gggaggagcg gctctggggg
    17521 gaaccctagc gccttgggcc tttttgtccc ctcaggcggg cctcgtggcg gggatcttcc
    17581 tgggggcctg gacggcggcg cgaggaatgg gggcgctgga cgaagcctcc ggggtgcggc
    17641 gtctcctcgc cttggccctg ggggtcgcca ccctggggga agggtgggga ctcatgacgg
    17701 aaaggcggtc ctcggtaggg ctctggcacc tgctggaggg ccgatcgacg gggctgcgac
    17761 gtctgagttt cttcgtcgcc ttgacggttt tatttttcct cacccgcagg ccccggcgat
    17821 tctcccccgc cgcttacgag cgggttgcaa aaaattgcgc cctccaatgg ttggaggggg
    17881 ctcttcgggg cgtggccttt gccgaaaacg actttaccct ccgggagatg gagagccgaa
    17941 aaaaccttcc cacggcggaa gaacgggccc tgctggccct agccgaagag gttcgttcct
    18001 gggacccctc ctccctcgaa agcatccccc aggcgtggga agacctccac cgagagatgg
    18061 aatcccaggg gatctccggg gaaaaggacc tgccaaccca ctgg
//
LOCUS       DAOOER010000038        16298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_4079_length_16298_cov_8.975066, whole genome shotgun sequence.
ACCESSION   DAOOER010000038 DAOOER010000000
VERSION     DAOOER010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 16298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16298
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4079_length_16298_cov_8.975066"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="PL162_06300"
     CDS             complement(<1..804)
                     /locus_tag="PL162_06300"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HQF91666.1"
                     /translation="MIFFWAVFWGAAGTLFSMALAARWGVVDRPGTLKIHALPVPRLG
                     GLGIVLAFLALLPLPSWGAAGSLVMALVGVADDRWNLSPKHKLWGQLGGGLCLALPWI
                     LRGAPGTALIMLLVAVFLANAVNLLDGMDGLAAGVTTIGALGIALIHASRGLTTAVPL
                     ILAGASLGFLLWNLPPARTFMGDVGSLFLGGALAWQIGGVYSTDLSALLGCFFCVGVP
                     CFDTLLGIARRMAAGRPVLSGDRDHFYDQLARRWHDQNRAVRAAWLLAAI"
     gene            complement(821..2710)
                     /locus_tag="PL162_06305"
     CDS             complement(821..2710)
                     /locus_tag="PL162_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HQF91667.1"
                     /translation="MWSKPSAMFSALREAQRRIVTLWLFLARRRVAVLFCDAGALFLA
                     VYLGYALRFTIFFDLTQAGSNFFRALLVFEGTVLGTFWLGGVYRVLWSQASVEEYVHL
                     AGLYVLGVFLFGLADKMIPGFVMPRTSLAIMVVTGLLFVSALRASWRACARPICPPGP
                     RPRRTIIAGAGDAGAMLARELLRHEGDMAPVGFVDDDPQKGRMKVAGLPVLGPLDSLS
                     EVIAREEIQVVLVAMPSAPGHVVRRLLSFTGDASVEVRVLPSLRELAGGTLSVSRLRT
                     CRLEDLLCREPVKLDNDGIGEIIRGKVVLVTGAGGSIGSEICRQILDHAPGELLLLGH
                     GEHSLYCLGEEICRRAKETPMRLLVADVADPVAMAQIFSRYTPAVVFHAAAHKHVPLM
                     EHNAREALRVNALGGWTVARLAGEYGAERVVMISTDKAVNPTNVMGATKRVAELLLQE
                     AQEAYPGTAYMAVRFGNVLGSRGSVVPKFEQQIAAGGPLTVTDPEMRRYFMLIPEAVS
                     LVLQAGSLGRGGELFVLDMGDPVRIVDLAEMIIRLHGLRPGVDIPIVFSGLRPGEKLF
                     EELFYDPKSVSRTSHDKIFFTHLGGLQGAKLSQAVEEGLGGDDPGVREMLGRWVPTFR
                     GTERA"
     gene            complement(2670..3842)
                     /locus_tag="PL162_06310"
     CDS             complement(2670..3842)
                     /locus_tag="PL162_06310"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQF91668.1"
                     /translation="MDTRRIPLSSAQIVDDDVAAVVRALRSGRVAMGPEIRGFEEELA
                     AVAGVRHAVACSSGTAALHMALLALGVGEGDVVLTTPFSFVASSNVILMVGAVPVFVD
                     IDPGTLCPSPEELRRTIGALRRGELSFPNGERVAPHRLKALLLVDVFGLPAPWDQYEA
                     LAREEGLVLLEDSCEALGSRAFGRGCGSFGRAGAFAFYPNKQITTGEGGAVVTDDDEV
                     AALCRSYANQGRDDSGAWLAHVRLGYNYRMSELTAALGRSQISRLPAILERRRQVAAR
                     YGALLKDIEGLRLPESPPWCDRASWFVYVVLLPEGAPRDEVLRLLSRHGVENRAYFTP
                     IPLQPFYAHRFGYRRGDFPEMDRVCDRVAALPFYTDLSPEDQDHVVETLRHVLRPA"
     gene            complement(3963..4406)
                     /locus_tag="PL162_06315"
     CDS             complement(3963..4406)
                     /locus_tag="PL162_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91669.1"
                     /translation="MQMPMQIGLDELLSMVMARMEGMAMAEESLKTKFNVLARALYRK
                     GLLTDEDVVEALREEYRTLKELGVTGEVPGEDMIKAVAENILQWVRGDVPAIRQGMED
                     YEKKVRELSAQEARKPRIDVASPALLSQLDRATGKAPRGGSKLII"
     gene            4695..6722
                     /locus_tag="PL162_06320"
     CDS             4695..6722
                     /locus_tag="PL162_06320"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HQF91670.1"
                     /translation="MFSNDHIARAMTVRLDPVLPPMPPFEPGIRRAPDRGYSLSRAET
                     ELALRNALRYIPEELHAALAPEFLDELLTRGRIYGYRFRPAGPLRGKPIDAYRGKCLE
                     GRAFQVMIDNNLDFAVALYPYELVTYGETGQVCQNWMQYRLIMRYLEVLTDEQTLVVE
                     SGHPLGLFRSRKNSPRVIITNGLFVGMYDDPTNWHRAMQIGVSNYGQMTAGGWMYIGP
                     QGIVHGTFNTILNAGRLKFSLGNDGDLRGRLFVSSGLGGMSGAQPKAAEIAGAVGVVA
                     EVDPSRIRTRTDQGWVRFASSDLGEVFRIAREHLERREPTSIAYEGNIVDLLEYAVAH
                     GESIDLLSDQTSCHAPYDGGYCPQGVTFEERTALLREDREKFRRLVDQSLRRHFELVK
                     VLTDRGTYFFDYGNAFLKAVYDAGVPEVCKGADEKDGFVFPSYVEDIMGPMLFDYGYG
                     PFRWVCLSGDPEDLRKTDKAAMECIDPHRRPQDRDNWVWIRDAEKNRLVVGTQARILY
                     QDAEGRMKIAKTFNAMVRRGEIGPVMIGRDHHDVSGTDSPLRETSNIRDGSNITADMA
                     VQCYAGNAARGMSLIALHNGGGVGIGKSINGGFGLVLDGSARADEAIELSMAWDVMGG
                     VARRAWARCPHSIETSLRYNEAHPGTDHITLPFIPDDALVTDAVARALRGR"
     gene            6754..7455
                     /locus_tag="PL162_06325"
     CDS             6754..7455
                     /locus_tag="PL162_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91671.1"
                     /translation="MRSWKRAAMASILAVLLGVACHAPAWAKGDFYLRDLGGYVAFSR
                     GAGITAVEAMPERLWRIVDRGEMAYDLQEAFIHADSGSLNAYADPAWRAFVAEGRPLF
                     SFALEDRRGWGTLPTNVLGAVHIYDRLRDRFVRYASYASSWGVADLQVRIDVEDGGPY
                     DLDARANGVVRVGLAVGAGLTDVDDFTRGWGGGGGCTLGNSPGMAPLLLILPGAGAWA
                     WKRRRKGPQERGGPA"
     gene            7452..8804
                     /locus_tag="PL162_06330"
     CDS             7452..8804
                     /locus_tag="PL162_06330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91672.1"
                     /translation="MTEETPGLPISEELFAHLPVPALRGSIEDGVLVVSSVNGAFGEL
                     WPTQVPCPCTLETLFAGLPGPRDFTPLTSGGPPGWRHVLWELVPGENLVDLALHSTPE
                     GEIVAVMPECSEQMTSRRFHALREKASFLLRLHHAHEALAPLTSCITEFFGFDWTGIL
                     QWDSASCQWTPLTEAPHPFLLAPEDRRLESFLARLARDPHGEASGSRLGGLSVEEGAE
                     WWVRWSLEGGEWRDAMAEADLRSLFAGTLLGGTQPLVFLALSRREGALGRINGDALRA
                     LWPTLCSTAERYRATKAMAYLAQTDPETGLMAETPLRRRLQEELRRSRRYGYFLSLVR
                     LTLLNAREMAADDPENVADAMRSMGHDMGQSIRNVDVAGRTGPHAVVLALPHTDAPGR
                     KVVEGRLKDRISATSLFLSLPPEISLEGATFPDDGTPETFDEALGLLHADDAPLTALG
                     "
     gene            complement(8915..9817)
                     /locus_tag="PL162_06335"
     CDS             complement(8915..9817)
                     /locus_tag="PL162_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91673.1"
                     /translation="MAFRAQESLEELFQELYDSQDVAVAEDIAKKILVLDPDNPEALF
                     VLADGAEEYEAQAALLRRCVEETKRRMAQASPEEAESLEDLLFEAMRNLGWSLLLDEK
                     AGEALALAEEMLAFDGWDPSWGRGIRFGGLLAQGKFAETLEESLKAESGDLFAAHARA
                     VATLELAGPGADAYRAVWDAFRVAPDLPFFVLEYWDAPEEEDEEFLDDYNGALFLQLY
                     WTESEERIMVLSTATVFFGYLTDRLPDEVKEEVLANLRESSMFAELERARVELRERFG
                     PDGDVEQGDKEALKILAKMDLFVG"
     gene            complement(9879..11180)
                     /gene="glgC"
                     /locus_tag="PL162_06340"
     CDS             complement(9879..11180)
                     /gene="glgC"
                     /locus_tag="PL162_06340"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HQF91674.1"
                     /translation="MIYGRYGRVLGIVLAGGRGERLMPLTKYRSKPAVPFAAKYRIID
                     FALSNMVNSGLFSIYVLVQFKSQSLTEHIERGWQFGGAMRGRDFFVTSVPAQMWMGTH
                     WYKGTADAVLQNLHLITLFDADHVCIFAADHIYKMDLEQMLQFHIDGNADATVAANVV
                     PVAEASAFGCIGVDPHQRITSFVEKPANPPEIPGRPGFSYASMGNYIFRRQVLEEVLL
                     EDGSDEKSSHDFGKDILPKLFSRARLLAYDFPSQTLPGNDRPYWKDVGTIKAYWDAHM
                     DLLQHPSPLTLFNFQWPIRTVSFADPPGFTYPSQGKNCSVVGALRAEGSRVMGAVVHR
                     SVLSRNCVIQPGAVVEESIIGQGVMIGENCQIRRAIVDAHNVIPPNTVIGYNPEVDAE
                     HYTYDRESGVVVVSMPTIRLRSPQDAPAMYSLLQPGGGYVD"
     gene            complement(11346..12809)
                     /locus_tag="PL162_06345"
     CDS             complement(11346..12809)
                     /locus_tag="PL162_06345"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA];
                     GO_function: GO:0009011 - starch synthase activity
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HQF91675.1"
                     /translation="MDAAIRVLHVASEMAPLVKLGGLGDVVGSLPKALREAGVDARVL
                     LPCYAGLREHLAAKGFPLVPVKEGEIHVALNWRVYDGQVWETASDGVPVYLLDQPELF
                     GDPQVYPRESTLGATLPFAFLSLGALELARIWKPDIFHIHDWPGGLLPAALAWHRHYR
                     SLQQEYDTVLTIHNMAHQWILAPGALEAWGFGRDAFTMDRMEFFGSLNVLKGAIVASD
                     AVTTVSPRYSWEIQTSQGGFGLHGVLEDHRGKLSGILNGLDYALWNPESDAMIPAPFS
                     PRDMTGKGRCRQLLLERFGVEDDGRPILVFVGRLVEQKGVDILLASLEPFARMGARTL
                     VLGSGHAVFENALREAASRMGGTVGVHLGYDEELAHLLYAGGDILLMPSLFEPCGLSQ
                     LIALRYGTIPVVRAVGGLADTVIDADGSDDGNGFVFSDYVPHEFSHAVWRALERRRDG
                     AGWAQIQRRGMAQDYSWYRSAAAYAEVYRRLRGMGRE"
     gene            complement(12811..14547)
                     /gene="glgP"
                     /locus_tag="PL162_06350"
     CDS             complement(12811..14547)
                     /gene="glgP"
                     /locus_tag="PL162_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HQF91676.1"
                     /translation="MSVMVHGKNVGQSLRRMVENDPVFRTVAYFSMEIGINPQIPTYS
                     GGLGVLAGDILKSAADLGVPMVGITLLYKKGYFTQTFSPEGWQQEQEAVWEPNQFMQL
                     LPNEVSVEIEGRNVKIRAWVHDLIGQGGYPLPIYFLDTDCEGNSTEDRHFTWYLYGGD
                     QRYRLIQELILGVGGLRMLRDLGYDNARTYHLNEGHAGFLTLEMLREMGYDDFDKVRE
                     NVIFTTHTPVAAGHDHFPYDLVDRVMTPVFGSTLRRMLGPNGVSMTELGIRYSRYVNA
                     VSVKHAEVSRRMFNSSKVDAVTNGVHSTTWTCPGMARLFDKYIPGWRNDPTRLTQALL
                     LPDEAVWKAHQAAKMKLLAKVLEDTGQELSPDVLTIGFARRAATYKRADLILSDVRKL
                     VDACGGRVQFIFAGKAHPHDEGGKMVIQRLIQASREMRSVLPMVYLEGYDMDLSAILT
                     AGVDLWLNNPRRPREASGTSGMKCTHNGVMNFSVLDGWWIEGWVEGVTGWSIGPEPAE
                     ADLIEYDEMEDAIDLYAKLKEKIIPTYYDRRGEWISMMKRTIALNASYFNTHRVVKDY
                     CEKGYGVVFRGM"
     gene            complement(14757..>16298)
                     /locus_tag="PL162_06355"
     CDS             complement(14757..>16298)
                     /locus_tag="PL162_06355"
                     /inference="COORDINATES: protein motif:HMM:NF023478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HQF91677.1"
                     /translation="PTWEARIVENSSWSCAHGVERWRSDCGCHTGGEPGWHQRWRAPL
                     RRALDRFREVLDQEFEERLRPWCDDPWKLRDEAVALLLDEGGEEPLEFLRKRFGPLPP
                     GASRAILTLLEAQRMGMFQYTSCGWFFNDVAGIETAQILSYALRGVELLRSAGGPDLE
                     GALMEDLGAAEGNRPAFPTGAAVLEKMVAPGRRSLEDLAAVAMLRRGWESPFKKAGAP
                     LPDREGGPRSPAVQMVVQGRSWTSGELVLRGANLRLADRRTGEVWTGSGVVLSSGGLD
                     DVCHLRREETPSFEGLRRVFFEGDLMALGRTIERTFPLGPWHLDVLSRDDRWAVARDR
                     CVQAEQSQRGEACHALEDFKRLLMRLHSIGVEPPAILRAAAELCLSEQVRDLVKRGEE
                     TRSAEGGRGLDRALLELMEPGSPLGNLLEEAHALGVEPELSLLNPRLGDFFHDRLEDH
                     RLGRSSEAPYGELLALFRRTQELGMDPSLWRGQNELWRLLEGAGRTPGEEMLALARAW
                     GFATP"
BASE COUNT         2834 a         5657 c         5228 g         2579 t
ORIGIN      
        1 gatcgccgcc aaaagccacg ccgcccgcac cgcccggttc tggtcgtgcc accgccgggc
       61 gagctggtcg tagaagtggt cccggtcgcc ggaaaggacg ggcctgcccg cagccatgcg
      121 ccgggcgatg cccagcaggg tgtcgaagca ggggaccccc acgcaaaaaa agcaccccag
      181 gagggcggaa aggtcggtgc tgtagacgcc gccgatctgc cacgccaggg cccctcccag
      241 aaagaggctc cccacgtccc ccatgaaggt gcgggccgga ggaaggttcc acagcaaaaa
      301 gcccagggag gcccccgcga ggatcagggg caccgccgtg gtgagccccc gggaagcgtg
      361 gatcagggcg atacccaggg cgccgatggt cgtcaccccg gccgccaggc catccatgcc
      421 gtccaggagg ttcaccgcgt tagccagaaa gaccgccacc aggagcatga tgagcgccgt
      481 acctggggcc ccccgcagga tccagggcag ggccaggcag agccctccgc ccaattgacc
      541 ccagagcttg tgcttgggag agaggttcca ccggtcgtct gcgaccccca ccagggccat
      601 gaccaggctc cccgccgccc cccaggaggg aaggggtaaa agggccagaa acgccaagac
      661 tatccccagc ccccccagcc ggggcacggg gagggcgtgg atcttgagcg tccccggacg
      721 gtccaccacc ccccaccggg ccgccagcgc catggaaaag agggtccccg ccgctcccca
      781 gaataccgcc cagaaaaaga tcatcccctt ggtgcctcct ttatgccctc tccgtccccc
      841 gaaaggtggg gacccaccgc cctagcatct ccctcacccc ggggtcgtct ccccccaggc
      901 cctcctccac cgcctgggag agcttcgccc cctgaaggcc gccgagatgc gtgaaaaaaa
      961 tcttgtcgtg gctcgtccgg gagacgcttt tgggatcgta gaagagctcc tcgaagagct
     1021 tctccccggg gcgaagcccc gagaagacga tgggaatgtc tacgccgggg cgcaggccgt
     1081 ggagccggat gatcatctcc gccaggtcca cgatccgcac gggatccccc atgtcgagga
     1141 cgaagagctc tccccccctc cccagggacc cggcctggag caccaggctc accgcttcgg
     1201 ggatgagcat gaagtagcgt cgcatctcgg gatccgtcac cgtgaggggc cccccggcgg
     1261 cgatctgctg ttcgaacttg gggaccacgc tccctcgact tcccaggacg ttgccgaagc
     1321 gcaccgccat gtacgccgtg ccggggtagg cctcctgggc ctcctggagc aaaagctccg
     1381 ccacccgctt ggtggccccc atgacgttgg tggggttcac cgccttgtcc gtggagatca
     1441 tcaccacgcg ctcggccccg tattctcccg ccaggcgcgc cacggtccac ccccccaggg
     1501 cgttcacccg gagggcttcc cgggcgttgt gctccatgag gggcacgtgc ttgtgggcgg
     1561 cggcgtgaaa gaccaccgcc ggggtgtacc gggaaaagat ctgggccatg gccaccggat
     1621 ccgccacatc cgccaccagc aggcgcatgg gggtctcttt ggcccggcgg cagatttcct
     1681 ctcccagaca gtagagggag tgttctccgt gccccaggag gaggagctct ccgggggcgt
     1741 ggtccagaat ctggcggcag atctcgctgc cgatggaccc ccccgccccg gtgacaagca
     1801 ccaccttgcc ccggatgatc tccccgatgc catcgttatc cagcttcacg ggctcccgac
     1861 agagcaggtc ctccaggcga caggtgcgaa ggcggctcac ggagagggtg cctccggcga
     1921 gctcccgtag ggagggaaga acccgcacct ccaccgaggc atccccggtg aaagagagca
     1981 ggcggcgcac cacgtgcccc ggggcggagg gcatggccac caggaccacc tggatctcct
     2041 cccgggcgat gacctcggac aacgaatcca ggggccccag caccggaagc cccgccacct
     2101 tcatgcgccc cttctggggg tcgtcatcca cgaaccccac gggggccatg tccccctcat
     2161 ggcgcaggag ctcccgggcg agcatggccc ccgcatcccc cgccccggcg atgatggtac
     2221 gccgggggcg aggtcccgga gggcagatag gccgggcgca ggcccgccag gacgcccgga
     2281 gggcggagac gaagaggagg cccgtcacca ccatgatggc cagggaagtg cgaggcatga
     2341 caaagcccgg gatcatcttg tccgccaggc cgaacaaaaa cacccccagg acgtagagcc
     2401 cggcgaggtg cacgtactcc tccacgctcg cctgggacca gagcacccgg tagacccccc
     2461 ccagccagaa ggtccccagc accgttccct cgaagaccag cagggcccgg aagaaattgc
     2521 ttcccgcctg ggtcaagtcg aagaaaatgg tgaatcgcag ggcgtatccc aggtagaccg
     2581 ccagaaagag ggcccccgcg tcacagaaca gcacggcgac ccgccgccgc gccaggaaga
     2641 gccagagggt cacgatcctc ctttgggctt cacgcagggc ggagaacatg gcggagggtt
     2701 tcgaccacat gatcctgatc ctcgggggaa aggtcggtgt aaaagggcag agccgccacc
     2761 cgatcacaca cccggtccat ctcggggaag tctccccggc ggtacccgaa gcgatgggcg
     2821 tagaagggct ggaggggaat gggggtgaag taggcccggt tttccacccc gtgccgggag
     2881 agaaggcgca agacctcgtc ccggggggcc ccctcgggga ggagcaccac gtacacgaac
     2941 cagctcgccc ggtcgcacca ggggggggac tcggggagcc tcagcccctc gatgtctttg
     3001 agcagggcgc cgtaccgcgc agccacctgg cgacggcgct ccaaaatggc ggggaggcgg
     3061 gagatctggg atcgccccag ggccgccgtg agctcggaca tgcggtagtt gtaccccagg
     3121 cgcacgtggg cgagccacgc cccggagtcg tcccgcccct ggttcgcgta ggaacggcac
     3181 agggcggcca cctcgtcgtc gtccgtcacc accgcccccc cttcccccgt ggtgatctgc
     3241 ttgttcggat agaacgcgaa ggcccccgcc cggccgaagg agccgcagcc ccggccaaag
     3301 gcccggctcc ccagggcctc gcagctgtct tccagcagca cgagcccctc ttcccgagcc
     3361 agggcctcgt actgatccca gggggcggga aggccgaaga cgtccacgag gagaagggcc
     3421 ttgagccgat ggggggccac ccgctctccg ttgggaaagc tgagttcccc ccggcgcagg
     3481 gcgccgatgg tgcgccgtag ctcctccgga gaggggcaaa gtgtgccggg gtcgatgtcc
     3541 acgaagacgg gcaccgcccc caccatgagg atcacgttgc tgctcgccac aaagctgaag
     3601 ggggtggtga gcaccacgtc cccttcgccc acccccaggg cgaggagggc catgtggagg
     3661 gccgccgtgc cgctggagca ggccacggcg tggcggaccc ccgccaccgc cgccaattcc
     3721 tcctcgaagc cccggatctc gggccccatg gcgacgcggc cggacctgag ggcccgcacc
     3781 accgccgcca cgtcgtcgtc cacgatctgg gcgctggaaa ggggaatacg tcgggtatcc
     3841 atcctatcaa cctctctctt caaaagggcc cctcgaagag ctcctgggac ccttcactgc
     3901 ctccccggcc ttaccccgcg ccgggagggc ctttgatggc accaaaggga cggggccgcc
     3961 ggctagatga tgagcttgct tcctccccga ggggcctttc ccgtggcccg atccagctgg
     4021 gagagcaagg cgggagacgc cacgtcgatg cggggcttac gggcctcctg ggcggagagc
     4081 tcccggacct tcttctcgta gtcttccatc ccctgacgga tggcgggtac gtcgccgcgc
     4141 acccactgga ggatgttttc cgccacggcc ttgatcatgt cctcccccgg gacctccccc
     4201 gtgaccccca gctccttgag ggtacggtac tcctcccgca gggcctccac cacgtcttcg
     4261 tccgtcagga gccccttgcg gtaaagggcc cgggccaaca cgttgaactt ggtcttgagg
     4321 ctctcttccg ccatggccat gccctccatc cgggccatca ccatggaaag gagctcgtcc
     4381 aaaccgatct gcatgggcat ctgcatcttt tttcgacctc ccaaagtaaa ggggggcggg
     4441 ggcttcgccc cccgatcccc gtaagacacg gacagttatt ttatcacaaa agtcccccga
     4501 cgggcctccg tcatcccctc tttgccgcaa aaatctctct cttttcgggg taaccccctc
     4561 caaagggagg ccttttcccc tctccgaggt atataatggg gtaccttagg aggaatcggg
     4621 gggaggtttc gccgccatct ccccggagct tcccccatga tagcacctga cgaaggacac
     4681 aaggagggat agagatgttt tccaatgacc acatcgcccg cgccatgacc gtgaggctcg
     4741 atcccgtgtt gccgcccatg cctcccttcg agcccggcat tcgccgcgcc cccgaccggg
     4801 ggtactccct gtcccgggcc gaaacagagc tcgccctgcg caacgccctg cgctacatcc
     4861 ctgaagagct ccacgccgcc ctggcaccgg agttcctcga cgagctccta acccggggaa
     4921 ggatctacgg ctaccgcttc cgtcctgcgg gacccctccg gggcaagccc atcgacgcct
     4981 atcggggaaa gtgcctcgaa ggccgggcct ttcaggtgat gatcgacaac aacctcgatt
     5041 tcgccgtggc cctctacccc tacgagctgg tgacctacgg cgaaacgggg caggtgtgcc
     5101 agaactggat gcaataccgc ctgatcatgc gctatctgga agtcctcacc gacgagcaga
     5161 ccctggtggt ggagtccggc caccccctgg ggctcttccg gtctcgtaag aactcccccc
     5221 gggtcatcat caccaacggc ctcttcgtgg ggatgtacga cgaccccacc aactggcacc
     5281 gggccatgca gatcggggtg tccaactacg gccagatgac cgcaggggga tggatgtaca
     5341 tcggccccca gggcatcgtc cacggcacct tcaacaccat cctcaacgcg ggacggctga
     5401 agttctccct gggaaacgac ggtgacctcc ggggaaggct cttcgtctcc tcggggctgg
     5461 ggggcatgag cggggcccag cccaaggcgg cggagatcgc cggcgccgtg ggggtggtgg
     5521 cggaggtgga tccctcccgg atccgcaccc gcaccgacca ggggtgggtg cgcttcgctt
     5581 cgtcggatct cggggaggtc tttcgcatcg cccgagagca cctggagcgc cgggagccca
     5641 cctccatcgc ctacgaaggc aacatcgtgg atctcctgga atacgccgtg gcgcacggag
     5701 aatccatcga cctgctctcg gaccagacct cctgccacgc cccctacgac ggggggtact
     5761 gcccccaggg cgtcaccttc gaggaacgca ccgcccttct ccgggaggac cgggaaaaat
     5821 tccgccgtct ggtggaccag agccttcggc gccacttcga gctggtgaag gtcctgacgg
     5881 accgggggac ctacttcttc gactacggca acgcctttct caaggcggtc tacgacgcgg
     5941 gggtccccga ggtctgcaag ggggcggacg aaaaggacgg cttcgtcttc ccctcctacg
     6001 tggaggacat catgggcccc atgctcttcg actacggcta cggtcctttt cgctgggtct
     6061 gcctctccgg cgatcccgaa gatcttcgca aaacggacaa ggccgccatg gagtgcatcg
     6121 atccgcaccg gcgcccccag gaccgggaca actgggtctg gatccgggac gcggagaaaa
     6181 accgcctcgt ggtgggcacc caggcccgga ttctttacca ggacgccgaa gggcgcatga
     6241 agatcgccaa gaccttcaac gccatggtac gccgggggga gatcggcccc gtgatgatcg
     6301 gccgggacca ccacgacgtc tcggggaccg attccccctt acgggagacc tccaacatca
     6361 gagacgggag caacatcact gcggacatgg cggtgcagtg ctatgcgggc aacgccgccc
     6421 gaggcatgag cctcatcgcc ctgcacaacg gcggtggagt gggcatcggc aaatccatca
     6481 acgggggctt cggcctggtc ctcgacggat cggcgcgggc cgacgaggcc atcgagctct
     6541 ccatggcgtg ggacgtcatg ggaggggtgg cccgccgggc ctgggcccgg tgcccccact
     6601 ccatcgaaac gagcctccgg tacaacgagg cccatccggg aacggaccac atcaccctgc
     6661 ccttcatccc cgacgacgcc ctggtgacgg acgccgtggc ccgggccctt cggggccgtt
     6721 agggcgccgg aaggtcgggg gggggaaaat accatgagat cctggaaaag ggccgcgatg
     6781 gcgtcgatcc tggccgtcct cctgggggta gcatgccacg ccccggcctg ggccaagggt
     6841 gatttctact tgcgagacct ggggggctac gtggccttct cccggggggc ggggatcacc
     6901 gccgtggaag ccatgccgga gcgcctttgg cgcatcgtgg accggggaga gatggcctat
     6961 gatcttcagg aggccttcat tcacgccgat tcgggatcgc tgaatgccta tgccgacccc
     7021 gcctggcggg ccttcgtggc cgaagggcgc cccctcttct ccttcgccct ggaggaccgc
     7081 cgagggtggg gcacgctgcc caccaacgtg ctgggggcgg tccacatcta cgaccgcctt
     7141 cgggaccgct tcgtgcgcta cgcctcctac gccagcagtt ggggggtggc ggacctccag
     7201 gtgcgcatcg acgtggaaga cgggggaccc tacgacctgg acgcccgggc gaacggcgtg
     7261 gtgcgcgtgg ggctggccgt aggggcgggg ctcaccgatg tggacgactt tacccggggc
     7321 tgggggggag ggggaggatg caccttggga aactccccgg ggatggcccc tctcctgctg
     7381 atcctccccg gagccggagc ctgggcctgg aaaaggcgac gaaaggggcc ccaggagcga
     7441 ggaggcccgg catgaccgaa gagacccccg gcctccccat ttcggaggag ctcttcgccc
     7501 acctccccgt gcccgcgcta cggggctcca tcgaagatgg cgtcctggtg gtgtcttcgg
     7561 tgaacggggc cttcggcgag ctctggccca cgcaggtccc gtgcccctgc acgctggaaa
     7621 ccctctttgc cgggctcccg ggcccccggg acttcacccc cttgacctcc ggaggccccc
     7681 cggggtggcg ccacgtgctc tgggagctcg tccccgggga gaatctggtg gacctggccc
     7741 tgcactccac cccggagggg gagatcgtcg ccgtgatgcc cgagtgctcc gagcagatga
     7801 cgtcccggag gtttcacgcc ctgcgggaaa aggcttcctt cctcctccgg ctgcaccacg
     7861 cccacgaggc ccttgccccg ctgacgtctt gcattacgga gtttttcggc ttcgattgga
     7921 ccgggatcct ccagtgggac tccgcctcct gccagtggac ccccctgacg gaggcccccc
     7981 acccctttct cctggctccg gaggaccgtc gcctagagtc cttcttggcc cgtctggccc
     8041 gggaccccca tggggaggcc tccgggagcc gcctgggggg gctttcggtg gaagaagggg
     8101 cggagtggtg ggtgcgctgg agcctcgagg gaggggagtg gcgggacgcc atggcggagg
     8161 ccgacctccg atccctcttc gcggggaccc tgctcggggg aacccaaccc ctggtatttt
     8221 tggccctctc ccggcgggag ggggctttgg ggcgcatcaa cggcgacgct ctgcgggccc
     8281 tctggcccac cctgtgctcc acggcggaac ggtaccgcgc caccaaggcc atggcctacc
     8341 tggcccagac cgacccggag acggggctca tggccgagac cccgctgcgc cgccgcctcc
     8401 aggaggagct tcgccgcagc cggcgctacg gatacttcct gtcgctggtg cgcctcaccc
     8461 tgctcaacgc ccgggagatg gccgcagacg atccggaaaa cgtcgccgac gccatgagat
     8521 cgatgggcca cgacatgggc cagagcatcc gcaacgtgga cgtggcgggg agaacgggtc
     8581 cccacgccgt ggtcctggcg ctcccccaca ccgacgcccc gggacggaag gtggtggagg
     8641 gacgcctcaa ggatcgcatc tccgccacat ctttgttcct ttcccttccc cccgagatct
     8701 ccttggaagg ggccaccttc ccggacgacg gcacccccga gaccttcgac gaggccctcg
     8761 gcctgctcca cgccgacgac gcccccctca cggccctcgg gtgagccctc cgggtgagcc
     8821 tttcgggcct tcaaaaaaac cgcccccttt tcgggccccg acgccaaggc gccgaagctc
     8881 cgaaaagggg gcgctcttca tggcccccgc cggtctagcc gacgaagagg tccatcttgg
     8941 ccaggatttt cagggcttcc ttgtccccct gctccacgtc cccatccggg ccgaagcgct
     9001 cccgaagctc gacccgggcc ctttccagct cggcaaacat cgatgattcc cgtaggttgg
     9061 ccaggacctc ttctttcacc tcgtcgggga gacgatcggt gaggtagccg aaaaacaccg
     9121 tggcggtgga gagcaccatg atgcgctcct cgctctccgt ccagtagagc tggaggaaga
     9181 gggccccgtt gtaatcgtcc aggaactcct cgtcttcctc ctccggggca tcccagtact
     9241 ccagcacgaa aaagggcaga tcgggggcga cgcggaaggc gtcccacacg gcccggtagg
     9301 catcggctcc gggccccgcc agctccaggg tggccaccgc ccgggcatgg gccgcaaaga
     9361 ggtctccgct ttccgccttc agggattctt ccagcgtctc ggcgaacttc ccctgggcca
     9421 agagccctcc gaagcggatg cccctccccc aggagggatc ccatccgtcg aaggccaaca
     9481 tctcctccgc cagggccagg gcctccccgg ccttctcgtc caacagtaga gaccaaccca
     9541 agttcctcat ggcctcgaag agaaggtctt ccagggattc cgcctcttcc ggggaggcct
     9601 gggccatgcg ccttttcgtc tcctccacgc agcgccgtag cagcgccgcc tgggcctcgt
     9661 actcctccgc cccgtccgcc agcacgaaga gggcctcggg attatcgggg tcgagcacga
     9721 ggatcttctt cgcgatgtcc tcggcgaccg ccacgtcctg ggagtcgtag agctcttgaa
     9781 agagctcttc caatgattct tgcgcacgaa atgccatggg ggtctcctcc aaatgggaaa
     9841 attggggccg gagtgcggcc cctcaagggg caacgacgct aatccacgta ccccccgccg
     9901 ggttggagca gggagtacat ggcgggggcg tcctgggggc tccggagccg gatggtgggc
     9961 atgctcacca ccaccacccc ggactcccga tcgtaggtgt aatgctccgc atccacctcc
    10021 gggttatacc cgatgacggt gttcggggga atgacgttgt gggcatccac gatggcccgg
    10081 cgaatctggc agttctcgcc gatcatcacc ccctggccga tgatggattc ttccaccacc
    10141 gcccccggct ggatcacgca gttccgggac agcaccgacc ggtgcaccac ggcccccatg
    10201 acccggctcc cctcggccct caaggccccc accacggagc aattcttacc ctggctcggg
    10261 taggtgaacc ccgggggatc ggcaaaggac acggtacgaa tgggccactg gaaattaaaa
    10321 agcgtcaggg gcgaagggtg ctgcaacagg tccatgtggg cgtcccagta ggccttgatg
    10381 gtgcccacgt ccttccagta ggggcggtcg ttccccggca acgtctggct ggggaagtcg
    10441 taggccagga ggcgggcccg ggagaaaagc ttcgggagga tgtctttgcc gaagtcgtgg
    10501 gagcttttct cgtccgatcc gtcctccagc aggacctcct ccagtacctg gcgtcggaag
    10561 atgtagttcc ccatggaggc gtagctaaaa cccggcctcc cggggatctc cggggggttg
    10621 gcgggttttt ccacgaagct ggtgatccgc tggtggggat ccaccccgat gcagccgaag
    10681 gccgaggcct cggccacggg gaccacgttg gccgccacgg tggcgtcggc gttgccgtcg
    10741 atgtggaact ggagcatctg ctccaggtcc atcttgtaga tgtggtcggc ggcaaagatg
    10801 cagacgtgat ctgcgtcgaa gagggtgatg aggtgaaggt tctggagcac cgcgtcggcc
    10861 gtgcccttgt accagtgggt gcccatccac atctgggcgg ggacgctggt gacgaagaag
    10921 tctcgccccc tcatggcgcc gccgaactgc catccccgct cgatgtgctc cgtgagagat
    10981 tggctcttga attgcaccag gacgtagatg gaaaaaaggc cgctatttac catattcgac
    11041 agggcgaagt cgatgatgcg gtacttggcc gcaaagggca ccgccggctt cgaccggtac
    11101 ttggtcaggg gcatgagacg ctccccccgg cctcccgcca ggacaatgcc caaaacccgt
    11161 ccatatctgc catagatcac ggcaaactcc ctcctttcgg ctggtaaatc ccctaaaaga
    11221 accaggcctt tccctaaaaa agagctcccc tgccggtaag atagccgggg aaagggcttc
    11281 ggtcaacccg gaagaggggc ccggggggga aagggggctg aaatatctgc gccccccgaa
    11341 ggggctcact cccgccccat gccccggagc ctgcggtaga cctcggcgta ggcggcggcg
    11401 gagcggtacc aggagtagtc ctgggccatg cctcgccgct ggatctgcgc ccaaccggcg
    11461 ccgtcgcggc gccgctccag ggcacgccac accgcgtggg agaattcgtg gggaacgtaa
    11521 tcggagaaga cgaagccgtt gccgtcgtcg gagccgtcgg cgtcgatgac cgtatccgcc
    11581 aaccccccca cggccctcac cacggggatg gtgccgtagc gcagggcgat gagttgggat
    11641 aggccacagg gttcgaagag ggagggcatg aggaggatgt ctcccccggc gtagagcaga
    11701 tgggccagct cttcgtcgta ccccaggtgg acccccaccg tcccccccat gcgggaggcc
    11761 gcctccctca gggcattctc gaagaccgca tgccccgacc ctaggacgag ggtccgggcc
    11821 cccatccggg caaagggttc cagggacgcc aggaggatgt ccacgccctt ttgttccacc
    11881 aggcggccga cgaagacgag gatggggcgg ccgtcgtctt ccacgccgaa acgctccagc
    11941 aggagctggc ggcagcgccc cttccccgtc atatcccggg gggaaaaggg ggccggtatc
    12001 atggcgtcgc tctccgggtt ccacagggcg tagtccaggc cgttcaggat gccggaaagc
    12061 tttccccggt gatcttccag cacgccatgg aggccgaagc ccccttgaga ggtctggatt
    12121 tcccaggagt accgggggct cacagtggtc accgcatccg aggccacaat ggcccccttg
    12181 agcacgttga ggctgccgaa gaactccatc cggtccatgg taaaggcgtc gcgcccaaaa
    12241 ccccaggcct ccagggctcc cggggccagg atccactgat gggccatgtt atggatggta
    12301 agcaccgtat cgtactcctg ctgcagcgac cggtagtgcc gatgccaggc cagggccgcc
    12361 ggcaggagcc cccccggcca gtcgtggatg tgaaagatgt cgggcttcca gatccgggcg
    12421 agctccaggg ctcccaggga gagaaaggcg aagggcaggg tggcccccag ggtagactcc
    12481 cgggggtaga cctggggatc tccgaagagc tccggctgat ccaggaggta caccggcacc
    12541 ccgtccgagg cggtctccca cacctggccg tcgtacaccc gccagttcag cgccacgtgg
    12601 atctcccctt ctttcacggg gacgagggga aaccccttgg cggcgaggtg ctctcgcagc
    12661 cccgcatagc aggggaggag gacccgggcg tccacgcccg cctcccggag ggccttggga
    12721 agagagccca ccacgtcccc cagccccccc agcttcacca ggggggccat ctctgaagcc
    12781 acatgcagga cccggatggc ggcgtccatg tcacatcccc cggaagacca cgccgtaccc
    12841 cttttcgcag tagtccttca ccacgcgatg ggtgttgaag tagcttgcgt tgagggcgat
    12901 ggtgcgcttc atcatggaga tccactctcc ccggcggtcg tagtaggtgg ggatgatctt
    12961 ttctttgagc ttggcgtaga ggtcgatggc gtcttccatc tcgtcgtact cgatgaggtc
    13021 ggcctcggcg ggctcggggc cgatggacca tcccgtcacg ccctcgaccc aaccctcgat
    13081 ccaccatccg tccaagacgg agaagttcat gacgccgtta tgggtgcact tcatgccgct
    13141 ggtccccgag gcctcccggg ggcgccgggg gttgttgagc cagaggtcca ctccggcggt
    13201 gaggatggcg gagaggtcca tgtcgtaccc ctccagatag accatgggga gcacggagcg
    13261 catctccctg gaggcctgga tgaggcgctg gatgaccatc tttcccccct cgtcgtgggg
    13321 gtgggccttg cccgcgaaga tgaactgcac ccggccgccg caggcatcca ccagcttgcg
    13381 cacatcggag aggatgaggt cggcccgctt gtaggtggcg gcccgccggg caaagcctat
    13441 ggtgaggacg tccggggaga gctcctgccc cgtgtcctcc aacactttgg ccaaaagctt
    13501 catcttggcc gcctggtggg ccttccagac cgcttcgtcg gggaggagca acgcctgggt
    13561 gaggcgggtg gggtcgttgc gccagccggg gatgtacttg tcgaaaaggc gggccatgcc
    13621 ggggcaagtc caggtggtag agtggactcc gttggtcacc gcgtccacct tagaggagtt
    13681 gaacatccgc cggctcacct cggcgtgctt caccgacacc gcattgacgt atcggctgta
    13741 ccggatcccc aactcggtca tggagacccc attggggccc agcatgcgcc gcagggtgga
    13801 gccgaacacg ggggtcatga cccggtccac cagatcgtag gggaagtggt cgtgccccgc
    13861 cgccaccggc gtatgggtgg tgaagatgac gttttcgcgc accttgtcga aatcgtcgta
    13921 acccatctcc cggagcatct ccagggtgag aaagcccgca tgcccctcgt tgaggtggta
    13981 cgtccgggcg ttgtcgtagc ccaggtcgcg gagcatgcgc aaccccccca cccccaggat
    14041 gagctcctgg atcaggcggt agcgctggtc tccgccgtag aggtaccagg tgaagtgccg
    14101 gtcttcggtg ctattgccct cgcagtccgt gtccaggaag tagatgggaa ggggataccc
    14161 cccctggccg atgaggtcgt gcacccaggc gcggatcttc acgttgcgcc cctcgatctc
    14221 cacggagacc tcgttgggga ggagctgcat gaactggttg ggctcccaga ccgcctcctg
    14281 ctcctgctgc cacccctcgg gggagaaggt ctgggtgaag tacccctttt tgtagagcag
    14341 ggtaatgccc accatgggaa cccccaggtc cgccgcgctc ttcaagatgt ctcccgccag
    14401 cactcccagc cccccggagt acgtgggaat ctgggggttg atgccgattt ccatggaaaa
    14461 gtacgccacg gtgcgaaaaa cgggatcgtt ctccaccatg cgacgaagcg attgccccac
    14521 gttcttgccg tgcaccatta cggacatgga cgggcctccc tcccaaaact tttttccgaa
    14581 gtccggggcg ccctaggccg ccccgtcgct aaaagctcct gccttctaag acctccgggg
    14641 cgtggcggag gggccatctt cgccccccca tccccgctcc cactctagca gaaaggcccc
    14701 tttagggaaa tgccccgggg aaaccctccg atgttttaaa cctccgtaag ggttcatcac
    14761 ggcgtggcaa atccccaggc tcgggccagg gccaacatct cctcccccgg ggtgcgcccg
    14821 gctccttcca gcaggcgcca cagttcgttc tggccccgcc aaaggcttgg atccatcccc
    14881 agctcctggg tgcgccgaaa gagggccagg agctctccgt agggggcttc ggaggatcgc
    14941 cccagacgat ggtcttccag gcgatcgtgg aagaaatccc ccagccgggg attcaacagg
    15001 gatagctcgg gctccacccc cagggcatgg gcctcctcca ggaggttccc caggggggat
    15061 cccggttcca tcaactccaa aagggcccga tcgagccctc gccccccctc ggccgacctg
    15121 gtctcttctc ctcgcttgac caggtcgcgc acctgctcag acagacagag ctccgccgcc
    15181 gcgcgcaaaa tggccggggg ctccactcct atggaatgaa ggcgcatgag gagacgttta
    15241 aaatcttcca aggcgtgaca ggcctctccc cgttgggatt gctccgcctg gacacagcga
    15301 tcccgggcca cggcccaccg gtcgtcccgg gacagcacat ccaggtgcca gggacccagg
    15361 ggaaaggtcc gctcgatggt gcgccccaaa gccatcagat ctccctcgaa aaagacccga
    15421 cgaagcccct cgaaggaagg ggtctcctcc cggcggagat ggcacacatc gtccaacccc
    15481 cccgaggaga gcaccacccc gctgccggtc cagacctcgc ccgtgcggcg gtcggcgagc
    15541 cgcaggttgg ccccccggag cacgagctcg ccgctggtcc agcttcgccc ctgaaccacc
    15601 atctgcaccg ccggagagcg gggccctccc tcccgatccg gcaagggagc gcccgctttc
    15661 ttaaaaggag attcccaccc ccttcgcaac atggccaccg ccgccaggtc ttccaggctc
    15721 cggcgccccg gggccaccat cttttccagc accgccgccc ccgtgggaaa ggcgggacgg
    15781 ttcccctccg ccgcccccag atcctccatg agcgccccct cgaggtcggg tccccccgcg
    15841 ctccggagca gctccacccc ccggagggcg taggaaagga tctgggcggt ctcaatgccc
    15901 gccacatcgt tgaagaacca gccgcaggac gtgtactgga acatgcccat gcgctgggcc
    15961 tccaggaggg tgaggatcgc ccgggaggcc ccggggggca gggggccgaa gcgcttccgg
    16021 aggaactcca ggggctcttc ccccccctcg tccaacagga gggccaccgc ctcgtcccga
    16081 agcttccagg ggtcgtcgca ccaggggcgg agcctctcct cgaactcctg gtccaagacc
    16141 tcccggaatc gatccaaggc ccggcgcagc ggggcgcgcc aacgctggtg ccaccccggc
    16201 tccccccccg tgtggcaccc gcagtcgctc cgccagcgct ccaccccgtg ggcgcaggac
    16261 caggagctgt tctccacgat gcgggcctcc caggtggg
//
LOCUS       DAOOER010000039        16223 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_4112_length_16223_cov_9.112197, whole genome shotgun sequence.
ACCESSION   DAOOER010000039 DAOOER010000000
VERSION     DAOOER010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 16223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16223
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4112_length_16223_cov_9.112197"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1052)
                     /locus_tag="PL162_06360"
     CDS             complement(<1..1052)
                     /locus_tag="PL162_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91678.1"
                     /translation="MNAIPLHSTVSLRFSPLPALLPPLLGYCRELARRLGFPDDEGAR
                     IELALEEALAALGDDAPDPEASVLVEFCRVPLGLRVRIGGYNRPRSREELSAYVPGSD
                     PDLGGSRCLEFFLLQRLVDQVSLVDREPGSQELHFFKRLPGALPHLLQEAGPHPRKGS
                     RAHRPSREPLRLPASTAREDAALLPEIRRRMPSEEAKGMALPSDLGMLQELLRDTEAA
                     IFATRDSEGALLAFQVVERYPHRRSLAVAHPPVVASDADFPQCGAALREAVCGHAAAS
                     GAGGVLLAAVAEEAGGPTVPPGFGVCALLPALPGTALWKGMPPSPRLLLFRPLGSEAS
                     RERLHLPPARRPLLLAL"
     gene            complement(1069..1509)
                     /locus_tag="PL162_06365"
     CDS             complement(1069..1509)
                     /locus_tag="PL162_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQF91679.1"
                     /translation="MRWFVMALALGWALGGGTAQAETAPPHPILSEDLVWSSPPALPA
                     LRSAWVLGGEAQTGPYLLRVRLAPGGVIPPHVHPDSRITTVLSGTLSVIFGTSPDEGT
                     PVALPTGGVYVAPAGVFHTLNARDGEVLYQESGTGPTGTTFYHP"
     gene            1841..2056
                     /locus_tag="PL162_06370"
     CDS             1841..2056
                     /locus_tag="PL162_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HQF91680.1"
                     /translation="MKNPGVPRDKRSSSTEIIKILEDDGWKLWKTEGSHYQYKHPSKK
                     EKVTVKHPDKDVPIKTERSIWNQAGLL"
     gene            2082..2537
                     /locus_tag="PL162_06375"
     CDS             2082..2537
                     /locus_tag="PL162_06375"
                     /inference="COORDINATES: protein motif:HMM:NF027249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HQF91681.1"
                     /translation="MDSHPNRYRYPAFLGVDEGTGRYYVLFPDLPGCTTTGDTEEEAL
                     SNAKEAMSLHLFGMEEDHDEIPAPSAISGLKGDDGEVVFLVDVWMPSFRERMETKAVH
                     RTVTLPGWLDHEAKLADLNYSQILQDGIMERLNISRTVTKRKRGKVLSR"
     gene            2964..5621
                     /gene="clpB"
                     /locus_tag="PL162_06380"
     CDS             2964..5621
                     /gene="clpB"
                     /locus_tag="PL162_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HQF91682.1"
                     /translation="MDLNKLTQKSQEALGEAQNLALSRGHQEVDGEHLVLALLRQEGG
                     LIPRLLRRMEASPEALGAAVERELDRRPRVKGPGVEPGKIYISGRLSRLLVGAEERAK
                     RLQDEYVSVEHLFAGLVDEGSATPAGRLLKEAGITEERFLGALTEVRGNVRVQSATPE
                     ATYEALERYGRDLVQMARSGKLDPVIGRDEEIRRVIRILSRKTKNNPVLIGEPGVGKT
                     AVVEGLAHRIVRGDVPEGLKDRTVMALDMGALVAGAKFRGEFEERLKAVLNEVKESEG
                     RVLLFIDELHTIVGAGKAEGSLDAGNMLKPLLARGELHCIGATTLDEYREHIEKDAAL
                     ERRFQPVLVDQPTVEDTISILRGLRERYEVFHGVRIQDNALVGAAVLSHRYITDRFLP
                     DKAIDLVDEACASIRTEIDSLPAELDSVARRVMQLEIEEAALAKETDRASCDRCAALR
                     KELAEARSRADALRAQYEVEKQSIGRVQALRSELEQTRAEAERAERSYDLNRAAELRH
                     GKLPELERRLRGEEARMTEHQGEARLLRQEVTEEEIAAIVSRWTGIPVARLVEGEREK
                     LLRLGEVLHQRVVGQNEAVDAVADAVLRARAGIKDPRRPIGSFLFLGPTGVGKTELAK
                     ALAAALFDSEENMVRIDMSEYMEKHTVSRLLGAPPGYVGYDEGGQLTEAVRRKPYSVL
                     LFDEVEKAHPDVFNVLLQILDDGRVTDSHGRTVNFKNTVLILTSNIGAGALMEGITAT
                     GEIPEGVRKTVWGELRRHFRPEFLNRLDDTVLFKPLQLAEIERIVELLTADLRRRLED
                     RRMTLELTPEVVKFVAREAYDPVYGARPLKRFLARELETPLARELVRGDLRDGAAITV
                     VLHEGRLALRHENPPEAPEEDVAEGEIVG"
     gene            5819..6361
                     /locus_tag="PL162_06385"
     CDS             5819..6361
                     /locus_tag="PL162_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6305 family protein"
                     /protein_id="HQF91683.1"
                     /translation="MKKIVLWWSVVVLLLLAGGAWAADIALTTVGQSPDAVMVKVLLK
                     RLGVEAVYEPLLKAEALGAEKVLIAVVGGSTKGLGAAGINAEDEKARTVSLLEAAKGK
                     GLHILVMHVGGEGRRGSLSDAFIQTAVPYAEEIILVQGANADGLFTKLAGTTPLVEVA
                     SVSAAQGPLGDVLKRWHVMP"
     gene            6371..7660
                     /locus_tag="PL162_06390"
     CDS             6371..7660
                     /locus_tag="PL162_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HQF91684.1"
                     /translation="MEWFWPEGALTLVMIGTFVFGAFALKLPIAVAMSLAAVAGALAA
                     GEGFPLRHLVEGTFGYLDTILIIASAMIFMKSLERIGLLEGLAAWLIRRFRNFPLLLS
                     FGLMGLIMFPGMITGSSTAAVLTSGALVSPVLIRLGVPPLKTAAAVAMGAIYGMIAPP
                     VNLPAMIIGGGIDMPYVGFAVPLLICTVPLAAVTALMLLFPSLRRGGQDLPALEAELE
                     RMERRPLTFRRFFPLLLLVVLLSGEQFFPQFWPPLGMPLDFLLAAGAALFSGERWNPW
                     ETACDAVRDALPVMGILMGVGMFIQIMTLTGVRGFVVVSALAIPAAALYVSIALSLPL
                     FGAVSAFGAASVLGVPFLLALLGRNEILVASALSLIAGLGDLMPPTALAGIFAAQVVG
                     ESNYFRVLRFCVVPGILTAAWGVAIIAGAKALAAVLY"
     gene            7671..7820
                     /locus_tag="PL162_06395"
     CDS             7671..7820
                     /locus_tag="PL162_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91685.1"
                     /translation="MMMMTIYRGIALLVLLLVGHAMFRERDFWKQVTGVLVLVPLILR
                     ILLVK"
     gene            7833..8987
                     /locus_tag="PL162_06400"
     CDS             7833..8987
                     /locus_tag="PL162_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HQF91686.1"
                     /translation="MFKKLSGTPLSALVLVLLSGVVALVTGKSFSAMHTLDPLVPAPG
                     FERHMLSEWFAPLRDTPGDTPVYIQRGSEPGGTVLVMGGTHPNEPAGTLTAVVLLERA
                     RINRGRLIVIPFANRSAFTCNDPQEGAPQSFSLRCADGSFRVFRYGSRATNPVDQWPD
                     PDVYLHPQSGQQLSGRERSNLNRAHPGTPQGPLTLQVAYALRRLLQEEKVDLAFDLHE
                     ASPEYPVVNAVVAHERAQELAAMVAMELEGLSIAIRIEPSPKNLRGLTHREWGDATET
                     LAVLMETANPSQGRLRGRTDEALVLSGRDKCYARAMELGRLFINYSGDQTLDLRVARH
                     TAAVKSFLENLGLLYPEKTVVMEEVPGFEELLARGVGAFLSPLSRDHGSR"
     gene            9031..10746
                     /gene="ggt"
                     /locus_tag="PL162_06405"
     CDS             9031..10746
                     /gene="ggt"
                     /locus_tag="PL162_06405"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HQF91687.1"
                     /translation="MRKVWVMVLVGVLAWALPGSALAEDIWAEHGMVSSAHRLASRAG
                     AEILTLGGNAVDAAVATALALNVVEPNASGIGGGGFMTIRLAETGEVVVLDYRETAPG
                     SATKDLFASEQAKTEKWSISGGKSVGVPGWLKGMWTALEKYGTMTFAQVAAPAIRLAE
                     EGFAVHPMQTGIIQDELERLMAYNDPATLPFLDEGLPLAAGKLLKQPALAKTFRLIAK
                     KGPGVLYGGPLGEAVVAAVNKAGGAMTLDDLRNYKMEVRKPVSGTYRGYSIFSVPPAS
                     SGGTHIIQVLNIMENFPVKQMGPNDAKTLHVLAEALKMVYADRGAYMADTAFVDVPLS
                     GLSSKAYAKKLADKIDMKSVMKDVKADDPKPFAEEKTVSYLGGDPQERISTSHFSVAD
                     AQGNIVASTNTINYFFGSAVFVPEYGFMLNDEMDDFSTNPESVNAPEPGKRPLSSMSP
                     TLVIDPSGRPFMTLGAAGATRIISAVAQIIMNTVDFGMTMDDAIEQVRIFNTVSGGKA
                     GHLMYEKGMSAGTVAYLQLLGHEVEEKDKSGYFGTAQGILFHAGKNMMDGGADSRRLG
                     VPVGY"
     gene            10922..11302
                     /locus_tag="PL162_06410"
     CDS             10922..11302
                     /locus_tag="PL162_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQF91688.1"
                     /translation="MRRAVVSEKAPKAVGPYSSAVWEGDLLFLSGQTPIDPATQRLVE
                     GDIGVQTGRAFDNLEAVLQDAGLSLDHVVKCNVYLTTMKDFAAMNDVYRTRFTPPYPA
                     RTTVAVAELPLGARVEIEMVARKA"
     gene            complement(12222..>13061)
                     /locus_tag="PL162_06415"
     CDS             complement(12222..>13061)
                     /locus_tag="PL162_06415"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HQF91689.1"
                     /translation="AIAHILGTLDDKIELNRRMNQTLEEMARALFRSWFVDFDPVRAK
                     AEGRETGLPEEIAALFPDEFEDSELGEIPKGWKVTPLSNQLNIIGGGTPKTSVAEYWN
                     GNIPWFSVTDTPAGSDVFVIATERTITEAGLLNSSARLVSKGTTIISARGTVGNLAIT
                     GCDMAFNQSCYGLKGIDNTGYYYVYLLALLMVERLKNMAHGSVFSTITRQTFEAIVRP
                     LPCYTTLRAFEQMVSPLFGAILSNVEQSRTFASIRDTLLPKLISGELRIEDAERFTEA
                     SGA"
     assembly_gap    13062..13161
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13162..14615)
                     /locus_tag="PL162_06420"
     CDS             complement(<13162..14615)
                     /locus_tag="PL162_06420"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HQF91690.1"
                     /translation="MANGNAKKNTTADIGFEQQIWAAADILRGNMDAAEYKHVVLGLI
                     FLKYISDKFEERYQELLAEDDDVEDKDAYVEMNVFFVPPEARWSVIATAAHTEEIGTV
                     IDDAMRAIEKENKRLKDILPKTYARTELDKRRLGNVVDLFTNIQMIEHGTDKDILGRT
                     YEYCLSRFAEQEGKRAGEFYTPSCVVRTLVEVLQPFQGRVYDPCCGSGGMFVQSTNFV
                     KNHAGNIGNLSVYGQDDNPTTRKMALMNLAIRGIEADLGGNNADTFRNDLHKTLKADF
                     ILANPPFNLKEWNDGSLNDDPRWKYGLPPSSNANFAWLQHMIHHLAPNGKIGMVLANG
                     SMSSNQSGEGEIRKKIIEADLVDCMVAMPGQLFYSTQIPVCLWFLARDKENTGFRDRR
                     GETLFIDARKMGTLIDRVHRELTDEDIRRIADTYHAWRGKAAAGGYEDVPGFCKSATL
                     EDIRHHGHVLTPGRYVGAEEIEDDGEPFEEKMARL"
     gene            15247..>16223
                     /locus_tag="PL162_06425"
     CDS             15247..>16223
                     /locus_tag="PL162_06425"
                     /inference="COORDINATES: protein motif:HMM:NF016220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HQF91691.1"
                     /translation="MAYTEANYENAIIDLFRYTLGYGHTYAPDLARDYTDPLYADELR
                     LALRHVNPKLPEAALTEAVYKLRNFEGGTFLQKNVQFMEYLQNGVSVNYYDKGEQRAA
                     LVYLVDYENVDRNTFTVANQWTMIENSEKRPDIIVFLNGLPVVIFELKSPSREETDAS
                     EAFLQLRNYMHEIPSLFIYNAFLVMSDLAISKAGSITADEDRFMEWKTKDGSYENTKY
                     AQFDTFIEGIFDKARLLDIIKNFICFSGDAKILGGYHQYFAVRKAVASTAKATVTDGK
                     GGVFWHTQGSGKSLSMVFYAHLLQDALNSPTLVVLTDRNDLDDQLFGQFA"
BASE COUNT         3010 a         5021 c         5132 g         2960 t
ORIGIN      
        1 agggcgagca gaagagggcg ccgggcaggg ggcaggtgga gcctttcccg gcttgcttcc
       61 gaccccaggg gtcgaaagag gagcaggcgg ggagatgggg gcatgccctt ccagagggcg
      121 gtgccgggga gcgcgggcag cagggcgcag accccgaacc ccgggggcac cgtgggacct
      181 ccggcctcct cggccacggc ggccaggagc acgccccccg cgccggatgc cgccgcgtgg
      241 ccgcacaccg cttcccggag ggcggcgcca cactggggga aatccgcgtc ggaggccacc
      301 acggggggat gggccacggc gagggaacgg cggtggggat agcgctccac cacctgaaag
      361 gccagaaggg cgccttcgga gtcccgggtg gcgaagatcg ccgcttccgt gtcccggagg
      421 agctcctgaa gcatgcccag atctgaaggc agggccatgc ccttcgcttc ctcggagggc
      481 atacgtcgcc gaatttccgg cagcagggcg gcgtcttccc gggcggtgga ggccggaagg
      541 cgaaggggct ctcgggaggg gcggtgggcg cgggaccctt tcctggggtg cggccccgcc
      601 tcttgcaaga ggtggggcaa tgcccccggg agacgtttga aaaagtgcag ttcctgggag
      661 cccggttccc ggtccaccag ggatacctgg tccaccaggc gctgcaaaag aaagaactcc
      721 agacaccgag agcccccgag gtccgggtcg gagcccggca cgtaggcgga gagctcctcc
      781 cggcttcggg gtcggttgta tccgccgatg cgcacccgaa ggcccagggg aacccggcaa
      841 aactccacca gcacgctggc ctcggggtcg ggggcgtcat cgcccaaagc cgccagcgct
      901 tcctccaggg caagttcgat gcgggccccc tcgtcgtcgg gaaagcccaa gcggcgtgcc
      961 agctcccggc agtaccccag gaggggaggc agaagggcgg gaaggggaga aaatcgaagc
     1021 gaaacggtgc tgtgcagagg aatggcgttc atgagggcct ccgaaaggtc aggggtgata
     1081 aaacgtggtt cccgtgggac ccgtgcccga ttcctgatag agcacctccc catctcgggc
     1141 gttcagcgtg tggaacaccc ccgccggagc gacgtacacc cctcccgtag gcagcgccac
     1201 cggcgtgccc tcgtccggag acgtgccgaa gatcaccgac agggttcccg aaagcaccgt
     1261 ggtgatgcgg gaatccggat ggacgtgggg aggaatgacc cctcccgggg cgaggcgcac
     1321 ccggagaaga taggggccgg tctgggcctc tccccccagc acccaggcgc tccgcagggc
     1381 ggggagggcg ggggggctcg accagaccag atcttcggag agaatggggt ggggtggggc
     1441 ggtctccgcc tgggcggtgc ctcctcccag agcccatccc agtgccaggg ccatgacaaa
     1501 ccaccgcatg tgaggtccct ccttttggaa tttccggcga aagatggaga aaacattccg
     1561 cagaagccat tctaacacag gaaaactccc tcttttcagg aaaaggggcg ggacggtctc
     1621 ttttttgacg gcctccgaca aaaagcttgc ggcgaggggg ctccgtcctc cgggccccag
     1681 gacgccctag acctcggttt ttccccggcg cctcatgggc ggaggtctcg tcgccgccga
     1741 aaggccgcgc cagggccccc agcagctttt cccttcttgc cagaaagatt gacacatact
     1801 tttagcatgt attatcgttc tctcgtcagg gggcgatgac atgaagaacc ccggcgtccc
     1861 ccgggacaaa cgctcctcgt ccacagagat catcaagatt ttggaagatg acgggtggaa
     1921 actctggaaa accgaaggaa gccattatca gtacaaacat cccagcaaga aggagaaagt
     1981 taccgtaaag catcctgaca aagacgtccc gatcaaaaca gagcgatcca tctggaatca
     2041 ggcggggctc ctgtagttcc gccagaaagg agtgaaaatc catggacagc catcccaaca
     2101 ggtaccgtta tcccgccttt ttaggcgttg acgagggaac agggcgctat tacgtcctct
     2161 tccccgacct tcctggatgc accaccaccg gcgacaccga ggaagaagcg ctttccaacg
     2221 ccaaagaggc catgtcgctc cacctcttcg gcatggagga ggatcacgac gaaattcccg
     2281 ctcccagcgc catttccggt ctcaagggcg acgacggaga agtggtcttt cttgtcgatg
     2341 tctggatgcc gtcgtttcgg gaacgcatgg aaacaaaagc cgtccaccgg accgtcaccc
     2401 tcccgggatg gcttgaccac gaggccaagc tggccgattt gaactattcg cagattcttc
     2461 aggatggcat catggaacgc ctgaacatca gccgcaccgt gaccaaacgg aaaaggggga
     2521 aggtcttgtc gcgctaaatc tccattcccc aatccaggaa ccatcccttc ccctctcggt
     2581 ggggcggagg ctttgcacca cgcgcccccc ccaccccccc atcggcaccc tccgaaagat
     2641 ccgcaagcgg cgatgccacc gggtagcggc cccacgtttt ccgtgttgcc cctgcccggt
     2701 gggcgtccca ggtccccacc aggagcaaaa ttttccgccc ccccgctccg gggacctaag
     2761 gagagcgacg tcccccccgc cgcgccgttg gggcgccccc ggcgggaact tgaggaagga
     2821 gggcgaccgg gggacttccc ccttgactga tcttccctga tcaatataat aggcgttgat
     2881 caactttgat caaaaagcgg cgcgcaaggg ccccagacct cacgggaggc ccccgggcac
     2941 gacaccgtcg aggagggatc gtcatggatc tgaacaaatt gacgcaaaaa tctcaggagg
     3001 ccctgggaga agcccagaac ctggcccttt cccgggggca tcaggaagtg gacggagagc
     3061 atctggtgct ggccctcctc cgccaggagg gggggctcat cccccgcctt ctccggcgca
     3121 tggaggcctc tcccgaagcg ctgggggccg ccgtggagcg ggagctggac cgacgtcccc
     3181 gggtgaaggg ccccggcgtg gaaccgggga agatctacat ctcggggcgc ctctcccgcc
     3241 ttctggtggg ggcggaggaa agggccaaac ggcttcagga cgagtacgtt tcggtggaac
     3301 atctctttgc ggggttggtg gacgagggga gcgccacccc cgccggtcgc ctgctgaagg
     3361 aggcggggat caccgaggaa cgctttttgg gggccctcac cgaggtgcgg ggaaacgtgc
     3421 gggtccagag cgccaccccc gaggccacct acgaagcctt ggagcgctac ggccgggatc
     3481 tggtgcagat ggcccggtcg ggcaagcttg accccgtcat cggccgggac gaggagatcc
     3541 ggcgcgtcat ccgcattctg agccggaaga ccaagaacaa ccccgtgctc atcggagagc
     3601 ccggcgtggg aaaaaccgcc gtggtggagg ggctggcgca ccgcatcgtc cggggcgacg
     3661 tccccgaggg gctcaaggac cgcacggtga tggccctgga catgggggcc ctggtggcgg
     3721 gggccaagtt ccggggggag tttgaagagc gcctcaaagc ggtgctcaac gaggtgaaag
     3781 agagcgaagg gcgggtgctg ctcttcatcg acgagctcca caccatcgtg ggggcgggaa
     3841 aagccgaagg atctttggac gcgggaaaca tgctcaagcc cctgctggcc cggggggagc
     3901 ttcactgcat cggcgccacc accctggacg aataccggga gcacatcgaa aaggatgcgg
     3961 cgctggagcg gcgctttcag cccgtgctgg tggaccagcc cacggtggag gacaccatct
     4021 ccatcctccg ggggcttcgg gagcggtacg aggtcttcca cggcgtgcgc atccaggaca
     4081 acgccctggt gggggcggcg gtgctctccc accggtacat caccgaccgc tttctgccgg
     4141 acaaggccat cgatctggtg gacgaggcct gcgcctccat ccgcacggag atcgactccc
     4201 tgccggcgga gctggacagc gtggcccgcc gggtgatgca gctggagatc gaggaggcgg
     4261 ccctggccaa ggagacggac cgggcgagct gcgaccgatg cgccgccctc cggaaggagc
     4321 tcgccgaggc gaggtcccgg gcggacgccc tccgggccca gtacgaggtg gaaaagcagt
     4381 ccatcggccg ggtgcaggcc ctgcggagcg aattggaaca gacccgggcc gaggcggaac
     4441 gggcggaaag gtcctacgac cttaaccgag cggcggagtt gcgccacggc aagctccctg
     4501 agctggagcg gcggcttcgg ggggaagagg cccgcatgac ggagcaccag ggggaggccc
     4561 gcctgctccg ccaggaggtc accgaagaag agatcgccgc catcgtgagc cggtggactg
     4621 gcattcccgt ggcccggctg gtggaggggg agcgggaaaa gctgctgcgc ctgggggagg
     4681 tgctccacca gcgggtggtg gggcagaacg aggcggtgga cgccgtggcg gacgcggtgc
     4741 tccgggcccg ggcggggatc aaggaccccc ggcgccccat cggctccttt ctcttcctcg
     4801 gccccacggg ggtgggcaag accgagctcg ccaaggccct ggccgccgcg ctcttcgaca
     4861 gcgaggaaaa catggtgcgc atcgacatgt cggagtacat ggagaaacac accgtgtccc
     4921 gcctgctggg ggcccctccg gggtacgtgg ggtacgacga gggggggcag ctcaccgagg
     4981 cggtgcgccg caagccctac agcgtgctgc tctttgacga ggtggaaaag gcccaccccg
     5041 atgtcttcaa cgtgttgctc cagattctgg acgacggccg ggtgacggac agccacgggc
     5101 gtacggtgaa cttcaagaac acggtgctca tcctcacgag caacatcggc gcgggagcgc
     5161 tcatggaggg gatcaccgcc acgggagaga tccccgaagg cgtgcgcaag acggtgtggg
     5221 gcgagctgcg gcgccacttc cgcccggagt ttctcaaccg gctggacgac acggtgctct
     5281 tcaagcccct ccagctggcg gagatcgaac gcatcgtgga gctcctcacc gccgacctcc
     5341 ggcggcgcct ggaagatcgg cgcatgaccc tggagctcac ccccgaagtg gtgaagttcg
     5401 tcgcccggga ggcctacgac cccgtctacg gcgcccggcc cctgaagcgt ttcctcgccc
     5461 gggagctgga aacgcccctg gcccgggagc tggttcgggg ggacctccgg gacggggcgg
     5521 cgatcaccgt ggtgctccac gaagggcgct tggccctgcg gcacgaaaat cctccggagg
     5581 ccccggagga ggacgtggcg gaaggggaaa tcgtgggata gtttttgaag gacgtgaaac
     5641 gcgaggggcc tccacgctgg aggcccctcg cgtttttccg ggggggggcc ccggcggcgg
     5701 agggaaacgt cttgcatctt ttctccaaca cgggtaccct aggacatatc atggcaactc
     5761 tgggacgcgc ggtgcgcatc gagcccgggg aaccttgatc tcaaaggagg gtttttagat
     5821 gaaaaaaata gtgttgtggt ggagtgtcgt ggtgctgctc ttgctggcgg ggggcgcctg
     5881 ggccgccgat atcgccctga ccaccgtcgg ccagagcccc gatgcggtca tggtgaaggt
     5941 gctcctgaag cgcctggggg tggaggccgt ctacgaaccg ttgctcaagg ccgaggccct
     6001 gggggcggag aaggtcctca tcgccgtggt gggggggagc accaagggcc ttggggcggc
     6061 gggcatcaac gccgaggacg aaaaggcccg gacggtgagc ctgctggagg cggcgaaggg
     6121 caaagggctc cacatcctgg tgatgcacgt ggggggagag ggacgccggg gatctctcag
     6181 cgatgccttc atccagaccg ccgtgcccta cgccgaagaa atcatcctcg tgcagggggc
     6241 caacgccgac ggccttttca ccaaactcgc cggcaccacg cctctggtgg aagtggccag
     6301 cgtctcggcg gcccagggtc ccctggggga tgtcctgaag cgctggcacg tcatgccgta
     6361 acctgagatc atggagtggt tctggcccga gggggccctc accctggtga tgatcggcac
     6421 ctttgtcttc ggcgccttcg ccctgaagct tcccatcgcc gtggccatgt ccctggcggc
     6481 ggtggcgggg gccctggccg ccggagaggg ttttcccctc cgccatctgg tggaggggac
     6541 cttcggctat ctggacacca tcctcatcat cgcctccgcc atgatattca tgaagtccct
     6601 tgagcgcatc ggcttgctgg agggactcgc cgcgtggctc atccgccgtt ttcggaactt
     6661 tcccctgctc ttgagctttg gcctcatggg gctcatcatg tttcccggca tgatcaccgg
     6721 atcttccacc gccgcggtgc tcacctcggg ggccctggtg tcgccggtgc tcatccgtct
     6781 gggggtgccg cccctcaaga ccgccgccgc cgtggccatg ggggccatct acggcatgat
     6841 cgcccctccg gtgaaccttc ccgccatgat catcggcgga gggatcgaca tgccctacgt
     6901 gggtttcgcc gtgcctctct tgatctgcac cgtgccgctg gcggcggtga cggcgcttat
     6961 gctgctcttt ccctctctgc ggcggggggg gcaggacctc cccgctctgg aggcggagct
     7021 ggagcgcatg gaacgccgcc ccctcacctt ccggagattt tttcccctgc ttctcctcgt
     7081 ggtcctgctc tcgggagagc agtttttccc ccagttctgg ccccccctgg ggatgcccct
     7141 ggattttctc ctcgccgccg gggcggcgct cttttccgga gagcggtgga acccctggga
     7201 aacggcctgt gacgcggtgc gcgacgccct tcccgtcatg ggaatcctca tgggggtggg
     7261 gatgttcatc cagatcatga ccctcacggg cgttcggggc ttcgtggtgg tctccgccct
     7321 ggcgatcccc gccgcggcgc tctatgtctc catcgccctc tcgttgcccc tcttcggggc
     7381 ggtctccgcc ttcggagcgg cttccgtgct gggggtgccc tttttgctgg ccctgctggg
     7441 acggaacgag atcctcgtgg cctccgccct gagcctcatc gccggtctgg gggatctcat
     7501 gccccccacg gcgctggcgg ggatcttcgc cgcccaggtg gtgggggaaa gcaactactt
     7561 ccgggtgctg cgcttctgcg tcgtgccggg gattctcacg gcggcgtggg gcgtggccat
     7621 tatcgccggc gccaaagccc tggccgccgt gctgtactag gaggcccgcc atgatgatga
     7681 tgacgatcta tcggggcatt gccctcttgg tgctgctgct ggtggggcac gccatgttcc
     7741 gggagcgcga tttctggaag caggtcacgg gggtgctggt gctggttccc ctgatcctgc
     7801 gcattctttt ggtgaagtag ggaggggagc ccatgttcaa aaaactctcc ggcacccccc
     7861 tgtcggccct cgtgctggtg ctcctctccg gagtcgtggc gctggtgacg ggcaagagct
     7921 tctccgccat gcacacgctg gatcccctgg tgcccgctcc gggcttcgag cgccacatgc
     7981 tctccgagtg gttcgccccc ttgcgggaca ctccggggga cacgccggtc tacatccagc
     8041 ggggatcgga gcccggaggc acggtgctgg tcatgggggg cacccacccc aacgagcccg
     8101 cggggaccct cacggcggtg gtgctcctgg agcgcgcccg cataaaccgg gggcggttga
     8161 tcgtgattcc cttcgccaac cgttcggcct tcacctgcaa cgacccccag gagggggctc
     8221 cccagagctt ttcgttgcgc tgcgccgacg gctccttccg ggtcttccgt tacggatccc
     8281 gggccaccaa ccccgtggac cagtggcccg atcccgatgt gtatctgcac ccccagtcgg
     8341 ggcagcagct ttcgggacgg gagcgcagca atctcaaccg ggcccatccg ggaacgcccc
     8401 aggggcccct caccctccag gtggcctacg ccctccggcg cctgctccag gaggagaagg
     8461 tggatctggc cttcgatctt cacgaggctt cgccggagta ccccgtggtg aacgccgtgg
     8521 tggcccacga acgggcccag gaactcgccg ccatggtggc catggagctg gaggggttga
     8581 gcattgccat ccgcatcgag ccgtcgccga aaaatctccg ggggctcacc caccgggaat
     8641 ggggggacgc caccgaaacg ctggcggtgc tcatggaaac ggcgaacccc agtcagggac
     8701 ggcttcgggg aagaacggac gaggccctgg tgctttcggg gcgggacaag tgctatgccc
     8761 gggccatgga gctggggcgc ctcttcatca actacagcgg ggaccagacg ctggacctgc
     8821 gggtggcgcg gcacaccgcc gcagtgaaga gctttctgga gaatctcggg ctgctctatc
     8881 ccgagaagac ggtggtgatg gaggaagttc ctggatttga ggaacttctg gcccgggggg
     8941 taggggcgtt cctgagtccc ctctcccggg atcatgggag ccgctgagga gctcatgcgg
     9001 cgcggacggt acggtaagga ggagagaagg atgcggaaag tgtgggtcat ggtgttggtg
     9061 ggcgtgctgg cgtgggctct gccgggaagc gctttggcgg aggacatctg ggcggagcac
     9121 ggcatggtgt cgtcggcgca ccggctggcc tcccgggcgg gggcggaaat cctcaccctg
     9181 gggggcaacg cggtggacgc ggcggtggcc accgctctgg cgctcaacgt ggtggaaccc
     9241 aacgcctcgg gcatcggggg cggggggttc atgaccatcc gcctcgcgga aaccggcgag
     9301 gtggtggtgc tggactaccg ggaaacggcg ccggggtctg ccaccaagga tctcttcgcc
     9361 tcggagcagg ccaagacgga aaagtggtcc atttccggcg gcaaatccgt gggggtgccg
     9421 ggatggctca agggcatgtg gaccgccctg gagaagtacg gcaccatgac cttcgcccag
     9481 gtggcggccc ccgccatccg gctggcggag gagggcttcg ccgtgcaccc catgcagacg
     9541 gggatcattc aggatgagct ggaacgcctg atggcgtaca acgatcccgc caccctgccc
     9601 ttcctggacg aagggctgcc cctggcggcg gggaaactcc tcaagcagcc cgctctcgcc
     9661 aagaccttcc gtctcattgc gaaaaagggc cccggcgtgc tctacggcgg ccctctgggc
     9721 gaagcggtgg tggcggcggt gaacaaggcc ggcggcgcca tgaccctgga cgatttgcgg
     9781 aactacaaga tggaagtgcg caagcccgtg tccggcacct accggggcta ttcgatcttc
     9841 tccgtgcccc ccgcatccag cggcggcacc cacatcatcc aggtcctgaa catcatggag
     9901 aacttccccg tgaagcagat ggggcccaat gacgccaaga cccttcacgt cctcgccgag
     9961 gccctgaaga tggtctacgc cgaccgggga gcctacatgg ccgatacggc ttttgtggac
    10021 gtgccccttt cggggctttc gtccaaggcc tacgccaaga agctcgcgga caagatcgac
    10081 atgaagtccg tcatgaagga cgtcaaggcc gacgacccca aacccttcgc ggaggagaaa
    10141 accgtctcct atctcggcgg ggatccccag gagcggattt ccaccagcca tttctccgtg
    10201 gcggacgccc aggggaacat cgtggcctcc acgaacacca tcaactactt cttcggcagc
    10261 gcggtcttcg tgccggagta cggtttcatg ctcaacgacg agatggacga cttttccacc
    10321 aaccccgaga gcgtcaacgc tccggaaccc ggcaaacgtc ccctgtcctc catgtccccc
    10381 accctggtca tcgacccctc ggggcgcccc ttcatgaccc tcggcgccgc gggagccacc
    10441 cgcatcatct ccgccgtggc ccagatcatc atgaacaccg tggacttcgg catgaccatg
    10501 gacgacgcca tcgagcaggt gcggattttc aacaccgttt ccggcggcaa ggcgggacat
    10561 ctcatgtacg aaaagggcat gagcgcgggg acggtggcct atctccagct cctggggcat
    10621 gaagtggaag agaaggacaa gagcggctac ttcggcaccg cccaggggat ccttttccac
    10681 gcggggaaga acatgatgga cggaggagcg gactcccggc gtctgggagt gcccgtgggc
    10741 tactgatctg atcgctgttc tccggccgga ggggatcttc ccctccggtt tttttgttgc
    10801 ccccggcgct tcgtcacccg ggccccttgt gacgagttct tgcgggtgtt aagatagatc
    10861 tgtggtggaa atgttcacca taatcgcggt ttattattgc caatcctcag gagggatgtc
    10921 tatgcgtcgc gcggtggtca gcgaaaaagc ccccaaagct gtggggccgt actcttcggc
    10981 ggtgtgggaa ggagatctgc tcttcctttc ggggcagacc cccatcgacc ccgccaccca
    11041 gcgtttggtg gagggggaca tcggagtcca gaccggccgg gccttcgaca atcttgaggc
    11101 ggtgctccag gacgcgggac tttctttgga tcatgtggtg aagtgcaacg tgtacctcac
    11161 caccatgaag gacttcgccg ccatgaacga cgtgtaccgc acccggttca ccccccccta
    11221 tccggcgcgc accacggtgg cggtggcgga acttcccctg ggggcccggg tggagatcga
    11281 gatggtggcc cgaaaggcct aggggcgaga aacttccggg gggctccttc acacggaaaa
    11341 gcgctgggag cgcgcacctt ccagccggct cctttcttcc ccacatccga aaaagggaaa
    11401 aatccgtagg ggcgaaaaat acgctcgccc ctacaccgca ccctccgcag accatccatc
    11461 catccctcca cacgggaaag cgggtgccgg gagcgtcggc ttccagctgg ctcctttcat
    11521 ccccacatcc gaaaaagggg aaaatccgta ggggcgaaaa atttttcgcc cctacaaaag
    11581 accgccacat tcatcagatt atctccaccg ccggagtcgt caggtgtgcc gtttttgaaa
    11641 aaacctacac tgcaccctcc gcagaccatc catccatcct tacgcgcgaa agctggacgc
    11701 tgggagcgcg cacattccag ccggctcctt tcttccccac atccgaaaaa gggaaaaatc
    11761 cgtaggggcg aaaaatacgc tcgcccctac accgcaccct ccgcagacca tccatccatc
    11821 cctccgcacg aaggcgggtg ccgggagcgc gcaccttcca gccggctctc ttcatcccca
    11881 cgcacaaggg gagccacacg gcgcggccct tcgcgcgcgg tgtggcgcgc ttccgatccg
    11941 cagacgtatc ctcctcccct gggaagactc ctcgatgtca ccctggagaa cctccaccgc
    12001 acaagcaaaa aaaggggccc ctaccggagc ccccttctca cggcgaaggt caaagggaag
    12061 gggccccgcg ggggcccctt ctgataatcg ctccctctcc ggaagggtgg ctctctttca
    12121 tcggaacagt ggctccattc catcggactg gtggctccat ttactacggt gtatccaaaa
    12181 accgagacgg actgctccgt ccatgctcaa tgaaacgtcg ctcatgcccc gctcgcctcc
    12241 gtgaaccgtt ccgcatcctc gatccggagt tctccagaga tgagcttggg gaggagagtg
    12301 tcgcggatgg aggcgaaggt gcgggattgt tcaacgttgg acaaaattgc accaaacaat
    12361 ggggaaacca tctgctcaaa agctcggagc gtcgtatagc aaggtaaagg tcgtacaatg
    12421 gcttcgaatg tttgtcgcgt tatggtagaa aaaacggaac cgtgggccat gttttttagt
    12481 cgttcaacca ttagtaaagc aagcaaataa acgtagtaat atcctgtgtt gtcgattcct
    12541 tttagcccat agcatgattg attaaatgcc atatcacaac cagtaatggc taagtttcca
    12601 acagttcccc tagcggaaat tatcgtagtc cccttagaaa ctagtcgggc ggaactgttc
    12661 aataacccgg cttctgttat tgtcctttct gtagctatca cgaaaacgtc actacctgca
    12721 ggggtatcgg taacagaaaa ccaaggaatg tttccgttcc aatattcggc gactgaggtc
    12781 tttggagtac caccaccaat aatgtttagc tgattactca atggtgtcac cttccacccc
    12841 ttcgggatct ctccaagttc ggagtcctcg aactcgtcgg ggaagagggc ggcgatttcc
    12901 tccgggaggc ctgtctcgcg cccctccgcc ttggcgcgca ccgggtcgaa gtcgacgaac
    12961 cagctcctga aaagggcgcg ggccatctct tcgagcgttt ggttcatcct gcggttgagt
    13021 tcgatcttgt cgtcgagcgt cccgaggatg tgggcgatgg cnnnnnnnnn nnnnnnnnnn
    13081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13141 nnnnnnnnnn nnnnnnnnnn nagccgcgcc atcttctcct cgaagggctc gccgtcgtct
    13201 tcgatctcct ctgcgccgac gtatcggccg ggggtgagca cgtggccatg atgccggata
    13261 tcttccagcg tggcgctctt gcagaagccg gggacgtctt cgtaccctcc ggcggccgct
    13321 ttcccccgcc aggcatggta ggtgtcggcg atcctgcgga tgtcctcgtc cgtcagctcc
    13381 cggtgtaccc ggtcgatcag cgtccccatc ttccgggcgt cgatgaagag cgtttctccc
    13441 cgtcggtcgc ggaagcccgt gttctccttg tcgcgggcga gaaaccagag gcagacgggg
    13501 atctgggtag agtagaaaag ctgcccgggc atggccacca tgcagtccac caggtcggcc
    13561 tcgatgatct tcttgcggat ttccccctcc cccgattggt tggaggacat ggagccgttg
    13621 gccagcacca tgccgatttt gccgttcggc gcgaggtggt gaatcatgtg ctgaagccac
    13681 gcaaagttgg cattgctgga gggcggcaga ccgtatttcc accgcgggtc gtcgttcagc
    13741 gagccgtcgt tccactcctt aaggttgaac ggaggattgg cgaggatgaa gtcggctttc
    13801 agcgtcttgt gcaaatcgtt gcggaaggta tcggcgttat taccgccaag gtcggcttct
    13861 atcccgcgaa tagcgaggtt catcaaagcc atcttgcggg tggtcggatt gtcgtcctgc
    13921 ccatagacgg agaggttccc aatgtttcca gcatggttct tgacgaagtt cgtagattgc
    13981 acgaacatac cgccggaacc gcaacaaggg tcatatacac gaccttggaa cggctgcaag
    14041 acctccacga gcgtccgtac cacgcaagac ggcgtataga attcacctgc ccgcttgcct
    14101 tcctgctcgg caaacctgga gaggcagtat tcataggtac ggccgaggat atccttatcc
    14161 gtgccgtgtt ctatcatctg aatgttggtg aagaggtcaa ccacgttccc aaggcggcgt
    14221 ttgtccaatt cggtacgggc gtaggtcttg ggcaggatgt ccttcagccg cttattctct
    14281 ttttcaatcg cccgcatggc gtcgtcaatg accgtgccga tttcctcggt gtgggcggcg
    14341 gtggcgatga cgctccaccg cgcttcgggc ggcacgaaga agacgttcat ctcgacgtaa
    14401 gcgtccttgt cctccacatc atcatcttcg gcgagcagtt cctgatacct ctcctcgaac
    14461 ttgtccgaga tgtacttgag gaatatcagc cccagcacga cgtgcttata ctcggcggcg
    14521 tccatgttgc cgcgcaggat atccgccgcc gcccagattt gctgttcgaa accgatgtcg
    14581 gctgtcgtgt ttttttttgc gttaccgttt gccatataag ttcttctcct tcttgttcat
    14641 cttcatgtgt tcgtcatgct gcttcccctt ttggtgttat ccaggcaccg aaggggggga
    14701 atggaggatg ctcaccatgc cccaggtcca tgatatcaga aaagagtatt tcgaagaggg
    14761 catgaccctc agctccggat cccttttccc tttcttgcaa ttttccccgc catggggggg
    14821 gaaacgcctc ccagtcccac cctttcgacg cccttcgccc tcacccccgc ccttccccat
    14881 gcccagaaaa aaagtttttt tgtccgtcag aacccaccta aggtgtcgtt gtccggtgct
    14941 ataatcgtga aaaatgattt tgagggcgga gatttcgcag ggccattttt tctacccctg
    15001 ggagaagggc cgcgccaagg tgctgcctcc ggcaagtccc tccccgagaa atgttcccga
    15061 tgttcctgca aatacaggct aacaatgcag aaacagcccg tcttgccgaa ctgcgcgaca
    15121 cccttttatc ccgcccgatg tccggcgagc tatcctttga tgttacattc tccaaaatga
    15181 ggggcagata ctacgccgat attttataac gcacgaaatt tagatagata gaaggagata
    15241 acccttatgg catacaccga agcaaactat gaaaatgcta taattgatct tttccgctac
    15301 acgctggggt acggtcacac ctacgccccg gacttggcgc gggactacac cgacccgctg
    15361 tatgcggacg agctgcggct cgccctgcgt cacgtcaacc ccaaattgcc ggaagccgcc
    15421 cttaccgagg cggtctataa gctacgcaat tttgagggcg gcacgtttct gcagaagaac
    15481 gtccagttca tggaatacct ccaaaacggc gtgtccgtca attactacga caagggggaa
    15541 caacgggctg ctctggtcta cctggtggac tacgagaacg tcgaccgcaa taccttcacc
    15601 gtcgccaacc agtggacgat gatagagaac agcgaaaagc gcccggacat tatcgtgttc
    15661 ctgaatggtc tgcctgtggt catcttcgag ttgaagtcgc cgagccgcga agaaacagac
    15721 gcttccgagg cgttcctgca gttgcgaaac tatatgcacg agatcccgtc gctgttcatc
    15781 tataacgcat ttctcgtgat gagcgacctc gccatctcca aagccggaag catcacggca
    15841 gacgaagacc gtttcatgga atggaaaacg aaggacggca gctatgagaa cacgaagtat
    15901 gcccagtttg atacctttat tgagggcatc ttcgacaaag cacggctgct cgacatcatc
    15961 aagaatttta tctgcttttc cggcgatgcg aaaatcctcg gggggtatca ccagtatttc
    16021 gctgtgcgga aagccgtagc ctccaccgcc aaagccaccg tcacggacgg caagggcggt
    16081 gtattctggc atacccaagg cagcggcaag tcgctgtcga tggtgttcta tgcccacctc
    16141 ctgcaagacg ctttgaactc accgactctc gtggtgctga ccgaccgcaa cgacctcgac
    16201 gaccagcttt tcgggcagtt tgc
//
LOCUS       DAOOER010000040        15921 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_4208_length_15921_cov_9.266041, whole genome shotgun sequence.
ACCESSION   DAOOER010000040 DAOOER010000000
VERSION     DAOOER010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 15921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15921
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4208_length_15921_cov_9.266041"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(509..2014)
                     /locus_tag="PL162_06430"
     CDS             complement(509..2014)
                     /locus_tag="PL162_06430"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HQF91692.1"
                     /translation="MATFDMGSLIQQLRHPVCRSLLRDLSLDELRNMATHDSVPNNQG
                     SPAYVTRVQSRNAPFTEIVYEVEESHLTLLEQVWKYLRWQQMIRISTRIGSSSGCPLK
                     TNYYVTRRYARMAHMFAANFFTQADDDGADIVTVVVPEWHQRKVLVFPSHRVTLILGI
                     DYYGESKMATLRMAMHIAREDLRGLGLHAGSKIVTLRGDSGELQEKGMLVFGLSGTGK
                     TTITTADHGLVAPEGVEVLQDDINILMPDGQAFGTENNFYIKTDNVSKQLPLLGAAQD
                     PRAILENVWVDDDGNVNFDNHAITTNGRAIVPRGSIPNTSARIDLPRVDVICFNMRRY
                     DIPPVGRLVSAEQAAAYFMLGESTVTSAEDPARVGQAKRVVGFDPFVVDHHYKNGNRL
                     LQILRDNPHISCYVLSTGRVGGQDGANITPEVTFGCIEGILRGSLTWRYDDVLGYEVP
                     ANLPVSNAEALDPATYWSRDDYARLIGALRAERRDYLATFSGLAPAIVHAV"
     gene            complement(2168..2833)
                     /locus_tag="PL162_06435"
     CDS             complement(2168..2833)
                     /locus_tag="PL162_06435"
                     /inference="COORDINATES: protein motif:HMM:NF013378.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HQF91693.1"
                     /translation="MVQERRVADSPGDFLEPAREGRWELVEKRSRFIGDLRGAFSEEG
                     ARSILRTLAQETPEAHHHCWAYRVGFPQIREYWSDDGEPSGSAGKPILGALRRAEVTN
                     ALLVVTRYFGGIKLGVRGLIDAYGQTATGVLAATPLAWRRRERKGKVVLRYDLHQAFC
                     RRLQDLGVREERLVSTFGENVTVCFAVPLAGVQAVEGFCQEFSRRGGVESWQWEEPPG
                     AGD"
     gene            complement(2812..3306)
                     /locus_tag="PL162_06440"
     CDS             complement(2812..3306)
                     /locus_tag="PL162_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HQF91694.1"
                     /translation="MSHLFAPWRMAYIGTGGSSECIFCAIPAASAEDDGRNLLIHRGD
                     RCFVVMNRFPYNPGHLMVAPYRHVGAYDELTEEELLELNHLTQRSLRVLRGLMNPQGF
                     NLGVNLGKGAGAGFDGHVHMHVVPRWDGDTNFMPVLGDVRVIPEALDATCRRLGDAWS
                     KSAG"
     assembly_gap    4024..4123
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4124..5775
                     /locus_tag="PL162_06445"
     CDS             <4124..5775
                     /locus_tag="PL162_06445"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HQF91695.1"
                     /translation="LLLAGVVRGPEGGTPAAWGAPDFLSPEERAWLQARPTPLRIAPD
                     PHFPPLEFFDDGGNWRGIAPAYVALIEKALGVRFAIVRLESFQAVLDHARQRSIDIAT
                     TVIDTPERRQFFLFTSPYIRIPNVIVVRQDREGTLSIADLQSFPRVMYQGGYAIGDVL
                     KEHGVTHATPITDPAAALRDLALGRVDAMVGNLATISHYARQLNLANLRVAGDCGFDD
                     TLSFASRSDEPLLSSILEKALGTITFQERDAIRDEWIKLAPPRFYQQREFWLFLLGGV
                     GVFAFVVALLAGWSWTLRRQVARRTAELQQKNDEMTRFLYTVSHDLKSPLVTIKTFLG
                     YLRKDLQRQDAQALEQDLTFITEAANRMTLLLEELLELSRIGRHAAPFREVPLQEVLD
                     GALALVAGALAERRVEVITPREPVILFGDPERLVELYQNLLDNAVKFMGNQPSPRIDL
                     GWRADAGKIWLFVRDNGGGIDPVFREKIFDIFEKFHPEIEGVGMGLAIVKRIVETHGG
                     AITVSSPGPGGGATFSFFLAGTRGGPSPESPSSATNLRRSS"
     gene            5772..6242
                     /locus_tag="PL162_06450"
     CDS             5772..6242
                     /locus_tag="PL162_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQF91696.1"
                     /translation="MTKKTLTVLLVEDDPAHAEIVRRNFRDFSPENRLEHVSDGVSAL
                     NYIYRRGIFAEPGAAPRPDLVLLDLRLPGVDGLTVLRTMKEDPSLAPIPVVILTTSAA
                     ESDVVQAYGNQANSYLVKPADFDEFVSLMETLEIYWLKRNFFLEVPEKYGSHAR"
     gene            6232..6624
                     /locus_tag="PL162_06455"
     CDS             6232..6624
                     /locus_tag="PL162_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQF91697.1"
                     /translation="MPDRAFQVLLLEDDVAHATAARRALEDGELPCVLRVVSDRESFL
                     RQLESSPPDVVLLDLNIPGGRSLDLLPMLQAPRSYPVLILTSHGDELAAVEAIKNGAM
                     DYLVKSPEAFAGLPRSIARALREWRLIQ"
     gene            6652..8058
                     /locus_tag="PL162_06460"
     CDS             6652..8058
                     /locus_tag="PL162_06460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HQF91698.1"
                     /translation="MTLEKYRVLFDMFPLGVAITDSQGKIVEMNQEGRRLLDEASEEP
                     SPLDPLPRSPLGRMVRPDGTPLPREEHASFRALKERRPVHAEQRWVVRDDTPARWLDV
                     TAAPIPLEGYGVAVAFGDITPRKEVERALEESRQLFESVTHTSPALLWISGPDKKCTW
                     FNDPWLAFTGRTLAQEVGDGWAEGVHPDDLERCIATYHGAFDARKPFAMEYRLRRHDG
                     VFRWVLDQGHPRCAPPGTFLGYIGSVLDITAQKEVEQNLRQALEDRDALLREVHHRVK
                     NNLQVICSLISLKIDGAPSPATQDALRETLTRIRSMSLIHEHLYRRGTFAAISMATYV
                     TELAHHLQEIFAPPSGEVDLAVAIAPELRLSLDQAISCGLLLNELVTNALKHAFPTAA
                     PREDAPRIRVALELREHTFLLTVQDNGVGIGEDLMETDRRTLGLRLVQALVRQLEGTL
                     HFAPGPGCQITVEFPVGA"
     gene            complement(8025..8180)
                     /locus_tag="PL162_06465"
     CDS             complement(8025..8180)
                     /locus_tag="PL162_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91699.1"
                     /translation="MHGAKNGVVDAENDISSVTEENAAPAGAQASNGVPAGAGAFYAP
                     TGNSTVI"
     gene            complement(8589..9398)
                     /locus_tag="PL162_06470"
     CDS             complement(8589..9398)
                     /locus_tag="PL162_06470"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQF91700.1"
                     /translation="MTPLVRRFARSRTPDIPPVRIAYLEKGHPHPLEPSFVAIPGIWE
                     PAERARPLLEALEGHALAVSLRGRGESDTPATGYDLAHHVGDLEGVVEAAGVRKMVLL
                     GFSRGVGYALGFARRHRELLAGLVLVDSRPRHSAFPPGRADLWKAHRVEGRPVTDFIR
                     PEALEGMEREAREVIFWDDLPGFAMPVLVLQGTSPTRTPPSNLDDADAARYGELLPRG
                     EVERFENSGHLIPDDEPERYRATVCRFVAHCRDRIAGSAHLPAGMGVFPTG"
     assembly_gap    9623..9722
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9723..9911
                     /locus_tag="PL162_06475"
     CDS             <9723..9911
                     /locus_tag="PL162_06475"
                     /inference="COORDINATES: protein motif:HMM:NF023583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HQF91701.1"
                     /translation="IRSSVGPRGRTVAIERSSSSMITGEIRSRIDRIWDTMWSGGISN
                     PLSVIEQLTYLLFIKGLD"
     gene            9912..11264
                     /locus_tag="PL162_06480"
     CDS             9912..11264
                     /locus_tag="PL162_06480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HQF91702.1"
                     /translation="MHTRRAAQAARTGTPIREPLFAPHQDHLRWSRFRDAAPEQMYAT
                     VKDEVFPFIKALGRSGGPEEGDSTYVHHMKDALFTIPTSRVLANVVDQLDGIEMPDSD
                     TKGDLYEYMLGKIATAGQNGQFRTPRHIIRLMVEMTDPTPRDVLCDPACGTAGFLVAA
                     AEYLREHHHDAIYRTEESRRKYNAGTFHGYDFDSTMLRIGSMNMLLHGVENPDIRYRD
                     SLAQADRDDAERFSLVLANPPFAGSLDYESTARDLLQVVKTKKTELLFLALFLRLLQT
                     GGRAAVIVPDGVLFGSSNAHRELRKILVERQKLDAVISMPSGVFKPYSGVSTAILLFT
                     KTNSGGTDQVWFYDMEADGFSLDDKRTPQPERSDLPDILARWQNRGAEAHHRRTDQSF
                     LVPKAEIAGNDYDLSLNRYKEVVHEAVVHDAPREILQRLAALEEEIAEGRRELEGMLG
                     "
     gene            11261..12439
                     /locus_tag="PL162_06485"
     CDS             11261..12439
                     /locus_tag="PL162_06485"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HQF91703.1"
                     /translation="MKWPKTTLGSIVSFKGGGTPSKDKSEYWTDEIPWATVKDFKGLT
                     ISCTQDSISKKGLKESASNIIPAGHVIIPTRMALGKAAINTIDMAINQDLRALIPKKQ
                     IDTRYLLYSMLGLAKQIEQFGTGATVKGITQNKLAELEIPLPPLDEQRRIAEILDAAD
                     ALRAKRREALAQLDALLQSTFLDMFGDPVTNPKGWEVVTGAEAFEELAYGTSTKATSE
                     PSPGNLPILRIPNVINGSIDWSDLKFINLPPKVSNKFLLNKNDLLFVRTNGNPDYIGR
                     CARFDGHKKALFSSYLIRGRLYATSGLDSEFLKNHIEFKTYRHEVRKEARTTAGNYNL
                     STFGIRKFCFIKPPLSLQRRFAAIVEGVERQKARMRAHGAELDALFASLQSRAFDGEL
                     "
     gene            12654..>15921
                     /locus_tag="PL162_06490"
     CDS             12654..>15921
                     /locus_tag="PL162_06490"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HQF91704.1"
                     /translation="MSNFAFFAQDFAPIAEAAAQAERSMAGDPRAACFHARFALEAMV
                     HWLYRHDTLLRMPYDQSLGALLHDPVFQNRLPQVVFQKARLIQKVGNQAVHNAQAPRP
                     ADALQVVKELHHCCHWLARTYASAARRNAPAWSDDLVPSVPVPGELIPRQELEIREKE
                     LAEQNKALLKSQKERDELDAEIQALRHQLAEARAAAEQRPDDHDYSEAETRRALIDVE
                     LARAGWPLEERRNREYRVAMPKNTTGSADYVLWGDDNNPLAVVEAKRTTVDPLRGQQQ
                     AKLYADGLEATYGIRPLIFCTNGYKTWLWDDRIYPPREVAGFFKKDELSRLALRRTQR
                     RPLDVAQVRPAIVDRYYQKRAIGSIFTQFTAARRKALLVMATGTGKTRTAVALVDVLQ
                     RAGWVKRVLFLADRVALVHQAVGAFKKHLPESSPVNLVTERDAEGRVFACTYPTMMGL
                     IDETEGLQARFSPGHFDLVIIDEAHRSVYQKYGAIFRYFDALLVGLTATPREDVDKNT
                     YDLFELETGVPTDAYELAQAVRDGYLVPPRGEQVDLKFPREGITYDGLSDEEKAQWEN
                     LDWGDEDHAVAPPDRVDPAAINRWLFNRDTVDKMLRHLMEHGHKVAGGDRIAKTIVFA
                     RNHQHAQFIEARFNHHYPQYAGHFARVIDNSVSHAQSLIDDFSQADKAPHIALSVDML
                     DTGIDIPEVANLVFFKPVSSKIKFWQMIGRGTRLCPELFGPGDDKRDFRIFDFCANFD
                     FFREHPDQIAPAGDPAPLGARLFRARVRLLGYVQGTPALNADGTLGKALADGLHAEVA
                     AMNREHFAVRLELAAVERFQRREAWEHLTAPDREVLERQVANLPTTLPLEDEAPRRFD
                     LITLEMQLALVEGDLGLFESRRRRVVKMAALLEEKVAIPAVAAQRAYLGAMQESSFWD
                     GPSIAGLEDMRGRLRALTSFLDRRMRPVVYTDFNDAFQGVRDVEAVAMPKMTGAQYEK
                     KVNDFLRHHQDHLVIHRLRTHQPLTPQDLHGLEEILASLGEEDGPGLLSGLLAKSGAP
                     SVAHFVRSLVGMDRATAQGAFSQFLNDRSLTAPQIRFIEMIVDQLTSRGIVGASALYE
                     A"
BASE COUNT         2940 a         5346 c         4772 g         2663 t
ORIGIN      
        1 tttcggaggt cgccgcccgc cagacttggg cttccccccg accccccaag ggttcccacc
       61 ccgggaagac ccaccccgat cgcagtcccc tttgggcttg aaggccccgg cggccccctg
      121 gccatgggga tgtctcccct cggaaaagct tggcatccct aagcccggag cgcctccaga
      181 gaaaactccc cgcctggggc ggtggttttt ttgagaactc cggggctttt gccgtccccg
      241 gaggaatgga tggtcaatat agatcaaccc ttcgcacccc tttttatgga atgatccatc
      301 ccttttcccc tccccggggc ccttgctccg gcccagcgga gccgcccttg acgtcccctt
      361 ttgccgcgcg gggacgatgg cgaagggccg cagccccggg gccccctgta ccacctttag
      421 gctttaggcc aaaaaacgag cccccggcca aaggcgccgg gggctcgtgg gagcaaccgc
      481 taaacgaagt gacggggtga acgcggggtc acaccgcgtg aacgatcgcg ggggccagcc
      541 ccgagaaggt ggccaagtaa tcccggcgtt cggcgcgcag ggccccgatg agccgggcgt
      601 agtcgtctcg gctccagtac gtcgccggat ccagagcttc ggcgttggac accggcagat
      661 ttgcgggcac ctcgtagccg agcacatcat cgtaccgcca ggtcaggcta ccccgcagga
      721 tgccctcgat gcacccaaaa gtcacttccg gggtgatgtt ggccccatcc tgccctccca
      781 ctcgccccgt gctgaggacg tagcaagaga tgtggggatt gtcccggagg atctgcagca
      841 ggcggttgcc gtttttgtag tggtgatcca ccacgaaggg gtcgaagccc accacccgct
      901 tggcctgccc cacccgggcg gggtcttcgg cgctggtgac ggtggattcc cccagcataa
      961 agtaggccgc cgcctgttcg gcggagacga ggcgtcccac gggggggata tcgtagcgcc
     1021 gcatgttgaa gcagatcaca tccacccggg gaaggtcgat gcgcgccgag gtgttgggaa
     1081 tggagccccg ggggacgatg gcccgaccgt tggtggtgat ggcgtggtta tcgaaattca
     1141 cgtttccatc gtcatccacc cagacgtttt cgaggatggc ccggggatcc tgggcggccc
     1201 ccaggagggg aagctgcttc gacacgttgt cggtcttgat gtagaagttg ttctccgtgc
     1261 cgaaggcctg gccgtcgggc atgaggatgt tgatgtcgtc ctggagcacc tccaccccct
     1321 cgggggcgac gaggccgtgg tccgccgtgg tgatggtggt cttccccgtg cccgacaggc
     1381 cgaagaccaa catgcctttt tcctgcaatt cccccgaatc cccccgcagc gtcacgatct
     1441 tgctccccgc gtggagcccc agcccccgga ggtcctcccg ggcgatgtgc atggccatgc
     1501 gcagggtggc catcttgctc tcgccgtagt agtcgatgcc caggatgagg gtcacgcggt
     1561 ggctgggaaa gaccaacacc ttgcgctggt gccactcggg caccaccacg gtgacgatgt
     1621 ccgccccatc gtcgtccgcc tgggtgaaga agttggcggc gaacatgtgg gccatccggg
     1681 catatctccg ggtaacatag tagttggtct tcagggggca tcccgaggag gagccgatgc
     1741 gcgtgctgat gcggatcatc tgctgccatc gcaggtactt ccacacctgt tccaagagcg
     1801 tcaggtgcga ttcctccact tcgtagacga tctccgtaaa gggggcgttc cgagattgga
     1861 cccgagtcac ataggctggc gatccctggt tgttgggcac gctgtcgtgg gtcgccatgt
     1921 tccgaagctc atccagggaa agatcccgca aaaggctgcg gcaaacgggg tgtctcaact
     1981 gctggatcaa ggatcccatg tcaaaagtcg ccacgatgat gtttcctcct tcccgatcta
     2041 ggggccccgg agaggcggcg ctcctccgaa ggctccgcca ctaaatacct catgccgaca
     2101 attcggtctt cccgcccggg gacacaaaaa acctcaaagg ggcgtccccg gtcaccgccc
     2161 cccttcctta gtctccggcc ccagggggct cctcccactg ccagctctcc actccacccc
     2221 gccgggaaaa ctcctggcaa aagccctcca ccgcctggac ccctgccaag ggcacggcga
     2281 agcacaccgt cacgttctct ccgaaggtgg agacgaggcg ctcttctcgc actcccaggt
     2341 cttgcagcct gcgacaaaag gcctgatgaa ggtcgtatcg caacaccacc tttccctttc
     2401 gttcccggcg ccgccatgcc aggggagtgg cggcgaggac gccggtggcc gtctggccgt
     2461 aggcgtcgat gagccctcgc acacccaact tgatgccccc gaaatatcgg gtgaccacca
     2521 gcagggcgtt ggtgacttcg gcccgacgca gagctcccag gatgggcttc cccgcgctcc
     2581 ccgagggttc cccatcgtcg gaccagtact ctcggatctg gggaaagccc acccgatagg
     2641 cccagcagtg atgatgggct tcgggggtct cttgggccag ggtgcggagg atgcttcggg
     2701 ccccctcttc cgaaaaggcc ccccggagat ctccgatgaa gcgcgacctc ttctccacca
     2761 actcccagcg cccctctcgg gcgggctcca gaaagtcccc cggagagtcg gctacccggc
     2821 gctcttggac catgcatccc ccaagcgccg gcaggtggcg tccagggctt cggggatcac
     2881 ccgcacgtct cccaacaccg gcatgaagtt cgtgtcccca tcccaccgtg ggacgacgtg
     2941 catgtgcacg tgaccgtcga accccgcccc cgcccctttg cccaaattga cccccaggtt
     3001 aaacccctgg ggattcatca gcccccggag cacccgcagg ctgcgttgag tcaggtggtt
     3061 caattccagg agctcttctt cggtgagctc gtcgtaggcc cccacgtggc gatagggggc
     3121 caccatgaga tgccccgggt tataggggaa gcggttcatc accacgaaac acctatcccc
     3181 ccggtggatc aggaggtttc tgccgtcgtc ttcggcactg gcggcgggaa tggcgcaaaa
     3241 gatgcactcc gaagagcccc ccgtgccgat gtaggccatg cgccaagggg caaacagatg
     3301 ggacacgagc catcacatcc tctcttaaaa gccttggggg cacgcccccc tcgggaagcc
     3361 ccccgcgccc gcgccgcggg ccaaaagccg ccccatcgat ggagggcgac gaagggctcg
     3421 tctcccacat tagcccggat ccggagggcg cctcacgtgg aagttattat agcaaaaaaa
     3481 tgtttcacgt gaaacatttt ttactattaa taaaatttct tccccgccaa ggaaaagctc
     3541 cttccccctt cgcacgccgc cccctaaggg taccacgctt ccttgaaatt ttggacctat
     3601 tttttaggaa aaagcccttt ctttctcgca tcaccacctt gaatcgtccg ccaaagcccc
     3661 caaggggcac gaggagaccc ggggggcgcc cccggaggcc cgggacgaga aaagatctcc
     3721 catcgggcct cgcccgcgac ggccccgaga tgccccgccg ccagggcgag gcaggcctcg
     3781 ggcggcccac ggcccgccgc cggaaaaaac gccgcagtcc aaagcttcgg aagtattgcc
     3841 gaaggagggc ataagcccta cactggaggg gcgtcagagg acgcgggatc cgccgccgag
     3901 ggcgatctcg aggcggaagg ggcaaacccg gggatcctcc gtaaaaaaag cgcccctaag
     3961 gaggcgaggt ccgtgcgctc cgttcgtttg ctccgcgttc tcggcatcat ctttggcttc
     4021 ctcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcctcct gctggccgga
     4141 gtcgtccggg gcccggaggg tggcaccccg gcggcatggg gcgccccgga ttttctttcc
     4201 cccgaggagc gcgcctggct ccaggccaga cccactcccc tccgcatcgc tccggacccc
     4261 cactttcctc cgctggaatt ttttgacgac gggggaaact ggcggggcat cgccccggcc
     4321 tacgtggccc tcatcgaaaa ggccctgggg gttcgcttcg ccatcgtgcg cctcgaaagt
     4381 tttcaggccg tcctggacca cgcccggcag cggagcatcg acatcgccac caccgtcatc
     4441 gacaccccgg agcggaggca attcttcctc ttcacctccc cctacatccg catccccaac
     4501 gtcatcgtgg tgcgccagga tcgggagggg accctctcca tcgccgatct ccagagcttt
     4561 ccccgggtga tgtaccaggg ggggtacgcc atcggcgacg tcctgaaaga acacggcgtc
     4621 acccacgcga cccccatcac cgaccccgcc gccgccctgc gggatctggc cctggggcgc
     4681 gtcgacgcca tggtgggaaa tctggccacc atctcccact acgcccgcca actcaacctc
     4741 gccaatctcc gggtggcggg ggactgcggc ttcgacgaca ccctttcctt cgcttcgcgg
     4801 agcgacgagc ccctgctctc gtccatcctg gaaaaggccc tggggaccat caccttccag
     4861 gaacgggacg ccatccggga cgagtggatc aagctggccc cccctcggtt ctaccaacag
     4921 agggaattct ggctcttcct cctgggaggc gtaggggtct tcgccttcgt ggtcgccctg
     4981 ctcgccgggt ggagctggac cctgcggcgc caggtggccc gccgcaccgc agagctccag
     5041 cagaaaaacg acgagatgac ccgctttctg tacaccgtct cccacgacct caaaagcccc
     5101 ctggtgacca tcaagacctt tctgggctat ctccggaagg acctgcaacg ccaggatgcc
     5161 caggccctgg agcaggatct gaccttcatc accgaggccg cgaaccgcat gaccctcctg
     5221 ctggaggagc tcctggagct ttcccgcatc ggccgccacg ccgccccctt tcgggaggtg
     5281 cccctgcaag aggtgctgga cggcgccctt gccctggtgg cgggggccct cgccgagcgc
     5341 cgcgtggagg tgatcacccc ccgggagccg gtgatcctct tcggagaccc cgagcgcctc
     5401 gtggagctct atcaaaatct tctcgacaat gcggtaaaat ttatggggaa ccagccctcc
     5461 ccccgcatcg acctcggctg gcgcgccgac gccgggaaaa tttggctctt cgtccgggac
     5521 aacggcgggg gcatcgaccc cgtcttccgg gaaaagatct tcgacatctt cgagaagttc
     5581 catccggaga tcgaaggcgt gggcatgggg ctcgccatcg tgaagcgcat cgtggagacc
     5641 cacggcggcg ccatcacggt ctcctctccg ggtcccggag ggggggccac gttttcgttc
     5701 tttctggcgg gcactcgggg ggggccctcg cccgaatccc cttcttccgc aacgaactta
     5761 aggaggtcat catgacaaaa aagaccctga ccgtgctctt ggtggaagat gatccagccc
     5821 acgccgagat cgtgcgtcgc aactttcggg acttttctcc cgaaaatcgg ctagagcacg
     5881 tgtccgacgg cgtctccgcc ctgaactata tctatcgccg ggggatcttt gcggagcccg
     5941 gcgccgcgcc gcgccccgat ctcgtgctgc tggacctccg ccttcccggc gtcgacggcc
     6001 tgacggtgct gcgcaccatg aaagaagatc cgtccctggc ccccatcccc gtggtgatcc
     6061 tcaccacctc ggcggcggag tcggatgtgg tccaggccta cggcaaccag gccaacagct
     6121 acctggtcaa gcctgcggat ttcgacgagt tcgtctccct catggaaacc ctggagatct
     6181 actggctaaa aaggaacttc ttcctggaag ttccggaaaa atacggatct catgcccgat
     6241 agggcctttc aagttctgct attggaagac gacgtcgccc acgccaccgc agcccgtcgc
     6301 gccctggagg acggagaact tccctgcgtc ctccgggtgg tctccgaccg ggaaagtttt
     6361 ttgcggcagc tggagagctc acctccggac gtggtgctgc tggacctgaa catccccgga
     6421 gggcgctctc tggatctttt gcccatgctc caagcccccc gaagctatcc cgtgctcatc
     6481 ctcacaagcc atggagacga gctcgccgct gtggaggcca tcaaaaatgg cgccatggat
     6541 tatctggtca agtcccccga agccttcgcg gggctccccc gatccatcgc ccgggccctg
     6601 cgggagtggc gcctgatcca gtagcaccgc caggcccagg agcaactgca agtgaccctg
     6661 gagaagtacc gcgtgctctt cgacatgttc cccctggggg tcgccatcac cgactcccag
     6721 ggcaaaatcg tggagatgaa ccaagagggg cggcgccttc tggatgaggc ctcggaggag
     6781 ccctccccct tagaccccct tccgcgaagt cccttggggc gcatggtgcg ccccgacggc
     6841 acccccctgc cccgcgagga acacgcctcc ttccgggccc tgaaggaacg tcgcccggta
     6901 cacgccgaac aaaggtgggt cgtgcgagac gacacacccg cccggtggct cgacgtcacc
     6961 gccgccccca ttcccctgga gggctacggt gtggcggtgg ccttcgggga catcaccccc
     7021 cgcaaggagg tcgaacgggc cctggaggaa tcccggcagc tcttcgagag cgtgacccac
     7081 acctcccccg cgcttctgtg gatttccggc cccgacaaga agtgcacctg gttcaacgac
     7141 ccctggctcg ccttcaccgg ccgcaccctg gcccaggagg tgggcgacgg ctgggccgaa
     7201 ggagttcacc ccgacgacct ggagcggtgc atcgccacct accatggggc cttcgacgcc
     7261 cggaaaccct tcgccatgga ataccgcctc cggcgccacg acggagtttt tcgctgggtc
     7321 ctggaccagg ggcacccccg atgcgccccc ccggggacat tcttggggta catcggttcc
     7381 gtgttggaca tcaccgccca gaaggaggtg gagcaaaacc tccgtcaggc cctggaggac
     7441 cgggatgctc tcctccgcga ggtgcaccac cgggtaaaaa acaaccttca ggtgatttgc
     7501 agtctcattt ccctgaagat cgacggcgcc ccctcccccg ccactcagga cgccctgcgg
     7561 gaaaccctga cccgcatccg ctccatgagc ctcatccacg agcacctcta ccgccggggg
     7621 acctttgccg ccatctccat ggcgacctac gtcaccgagc tggcgcacca cctccaggaa
     7681 atcttcgcgc ccccctcggg agaggtcgac ctcgccgtgg ccatcgcccc ggagctccgg
     7741 ctctccctgg atcaggccat ttcctgcggc cttctgctca acgaactcgt caccaacgcc
     7801 ctgaaacacg cctttcccac cgccgcacct cgggaggacg ccccccggat ccgcgttgcc
     7861 ctggaactcc gggaacacac cttcttgctg acggtgcagg acaacggggt ggggatcggg
     7921 gaagatctca tggagacgga ccgccgcacc ctggggctgc gcctcgtcca ggccctcgtg
     7981 cggcagctgg agggcaccct gcactttgcc ccggggcccg ggtgtcagat caccgtggag
     8041 ttccccgtgg gggcgtaaaa ggcccccgcc cccgccggga ccccgttcga ggcctgggcc
     8101 ccggcggggg cggcgttctc ctcggtcacc gaagaaatgt cgttctccgc gtccaccacg
     8161 ccgttcttcg ccccatgcaa taaggcagac cgacgctctc cgccagagag gccaggaacg
     8221 ccacgccgag gagcacgatg ctccgacggc gcgccgccgg ggtcctttcc cgctcgccgc
     8281 ccacaaaggc gtggatgtcg tcaacggagt tccccgtgcc gacaatccca tggacgaaca
     8341 agagacgccc caagcccccg caggggacgg cgtccgaagc ggactctgga aaggccctga
     8401 aagaaggggg ggcatcttca cggtggatgc ctcctggggg gagagggagg gagagatggt
     8461 ctgcgaaagg ggcgtgtggg aagcggtgga tgctctgcga aaggggcgtg taggggcgaa
     8521 aaatatgctc gcccctacgt tttcaccccg cgtcaccggc ccgggtcgga ggggcgcccc
     8581 tacgggtttt accctgtcgg gaaaaccccc atgccggctg gaaggtgcgc gctcccggcg
     8641 atgcgatccc ggcagtgcgc cacgaagcgg cataccgtgg cgcggtagcg ctcgggctcg
     8701 tcgtcgggga tcaggtgccc cgagttctcg aagcgctcca cctctccccg gggaaggagc
     8761 tccccgtacc gggctgcgtc ggcgtcgtcc aggttcgacg gaggggtccg ggtgggagaa
     8821 gttccctgga gcaccagcac cggcatggcg aacccgggga gatcgtccca gaagatcact
     8881 tcccgggcct cccgctccat gccctccagg gcctcggggc ggatgaagtc ggtgacgggg
     8941 cgcccctcca cccggtgggc cttccagaga tcggccctcc ccggcggaaa agcgctgtgc
     9001 cgggggcggg agtccaccag cacgagcccc gccaggagct cccggtgcct ccgggcgaac
     9061 cccagggcat atcccacccc ccgggagaag cccaagagca ccatcttccg cacccccgca
     9121 gcctccacca ccccctccag atcccccacg tggtgcgcca gatcgtatcc cgtggcgggg
     9181 gtgtcgctct ctccccgccc ccggaggctc accgccaggg cgtgcccctc cagagcttcc
     9241 aggaggggcc gggcccgctc ggcgggctcc cagatcccgg ggatcgccac gaaggagggt
     9301 tccagcggat gggggtgtcc cttttcgaga taggcgatcc gcaccggcgg gatgtccgga
     9361 gtgcggcttc gggcaaagcg gcggaccaaa ggtgtcatgg ggacctcctt caaggaagat
     9421 gcctttcgag catagcacgg cgccgacaaa agagaagagc ttgataagaa tcccccaccc
     9481 gccccctggc gttccgtccg tttttgacgg agctccatac cgagagcccc aggagggcaa
     9541 ggggccgcca ccagaccgta cgggaagcgt cggaaatcgc ggcagaaaac gaccgggggg
     9601 agctcttgca tcgccacaag gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnatccggag ctccgtcggc ccccggggcc gcaccgttgc catcgaaagg agttcatcat
     9781 ccatgatcac cggagaaatc agatcccgca tcgaccgcat ttgggacacc atgtggtccg
     9841 ggggcatctc aaaccccctt tcggtcatcg aacagctcac ctatctgctc ttcatcaagg
     9901 gcctcgacta ggtgcacacc cggcgtgcgg cccaagccgc ccgcaccggc acccccatcc
     9961 gggaacccct ctttgccccc caccaggacc atctgcgctg gtcgcgcttt cgcgacgccg
    10021 ccccggagca gatgtacgcc acggtaaagg atgaggtgtt ccccttcatc aaggccctcg
    10081 ggagatcggg gggcccggaa gagggggatt ccacctatgt ccaccacatg aaggacgccc
    10141 tgttcaccat ccccacctcc cgggtgctgg ccaacgtggt ggaccagctc gacggcattg
    10201 agatgcccga cagcgacacc aagggggatc tgtacgagta catgctgggg aaaatcgcca
    10261 ccgcagggca gaacggccag ttccgcaccc cccgccacat catccgcctc atggtggaga
    10321 tgaccgatcc caccccccgg gatgtcctct gtgaccccgc ctgcggcact gcgggatttc
    10381 tcgtcgccgc agcggagtac ctgcgcgagc accaccacga cgccatctac cgcaccgagg
    10441 aaagccgccg caagtacaac gcggggacct tccacggcta cgacttcgac tccaccatgc
    10501 tccgcatcgg cagcatgaac atgctgctcc acggggtgga aaaccccgac atccgctacc
    10561 gcgattccct ggcccaggcg gaccgagacg acgccgagcg cttttccctc gtcctcgcca
    10621 atcccccctt cgccgggagc ctggactacg aatccaccgc ccgggacctg ctgcaggtcg
    10681 tgaagaccaa gaagaccgag ctgctcttcc tcgccctctt tctgcgcctg ctccaaaccg
    10741 gagggcgggc ggcggtcatc gtccccgacg gggtgctctt cggctcgtcc aacgcccacc
    10801 gggagctccg gaagatcctc gtggagcggc aaaagctcga cgcggtgatc tccatgccct
    10861 cgggggtctt caagccctac tccggggtct ccaccgccat cctgctcttc accaagacca
    10921 actcgggggg cacggaccag gtgtggttct acgacatgga ggccgacggc ttttccctgg
    10981 acgacaagcg caccccccag cccgaaagga gcgacctccc cgacatcctc gcccgctggc
    11041 aaaaccgggg ggcggaggcc caccaccgcc gcacggacca gagctttctg gtgcccaagg
    11101 ccgaaattgc gggcaacgac tacgaccttt ctctcaaccg ctacaaagag gtggtgcacg
    11161 aggcggtggt ccacgacgcc ccccgggaga tcctgcagcg cctggccgcg ctggaggagg
    11221 agatcgccga ggggcggagg gagctggagg ggatgctggg atgaaatggc ccaaaactac
    11281 cttggggagt attgtttcgt tcaaaggggg agggactccc agcaaggaca aatcagaata
    11341 ttggactgac gaaatccctt gggctaccgt gaaggatttt aaagggctta ctatttcttg
    11401 cacacaagat tcaattagca aaaaaggtct caaggaaagc gcatcgaata ttattcctgc
    11461 cgggcatgtt attattccaa ctcgaatggc ccttgggaaa gctgccatta acaccataga
    11521 catggcaata aatcaagatt tgcgcgcatt aattccaaaa aaacaaatcg acacaagata
    11581 tcttttatac tccatgcttg gtcttgctaa acaaattgaa cagtttggaa cgggcgccac
    11641 cgtcaagggg atcacacaaa acaaacttgc ggaactcgaa atccccctcc cacccctcga
    11701 cgaacaacgg cgcatcgcgg aaattctgga cgcggcggac gctctgcgcg ccaaacgtcg
    11761 ggaagcgctg gcacagcttg atgcgctgct ccaatccacg tttctcgaca tgttcggcga
    11821 ccccgtcacc aacccgaagg ggtgggaggt ggttacggga gcagaagcat ttgaggaact
    11881 agcctatgga acatcaacaa aggcgacaag tgaaccatca cccggcaacc tgcctatttt
    11941 gcgcatacca aatgttataa acggcagcat agactggtct gatttgaaat ttataaatct
    12001 gccaccaaaa gtgtcgaata agtttttgtt aaacaaaaac gacttgctct ttgtcagaac
    12061 aaatggaaac ccagattata tagggcgttg cgcaagattt gacgggcata aaaaggcatt
    12121 gttttcatct tacttgatac gtggacgcct ttacgccacg tcgggactag actctgagtt
    12181 tttgaaaaac cacattgagt ttaaaactta ccgacatgag gttaggaagg aagcaagaac
    12241 aactgcaggt aattacaatt tgagcacttt tggcatacgc aagttttgtt tcataaagcc
    12301 ccccctctcc ctgcagcgcc gctttgccgc catcgtcgag ggcgtggagc ggcagaaggc
    12361 gcggatgcga gcccatgggg cggagctgga cgccctcttt gcgtcgctcc agtcccgggc
    12421 cttcgacggg gagctgtgac cgccatggaa aaaaccaccg tttccccctc tccgggaccc
    12481 cacgccggaa accccgggaa aaagccccgg gaaaccaggc tcccttgggc ttcgacggcg
    12541 agctcagaat atttcattcg gagctctgaa cacttggggc tgagctccga acatttaacc
    12601 ctgagctccg aacatttggg gatgaccccc ccgccccacc gaggccgccg atcatgagca
    12661 acttcgcctt cttcgcccag gacttcgccc ccatcgccga agccgccgcc caggccgaac
    12721 gttccatggc gggggatccc cgggcggcgt gcttccacgc ccggtttgcc ctggaggcca
    12781 tggtgcactg gctctaccgc cacgacaccc tcctccggat gccctacgac caaagcctgg
    12841 gggccctgtt gcacgacccc gtgtttcaga accgcctgcc ccaggtggtc tttcaaaagg
    12901 cccggctcat tcagaaggtg ggaaaccagg cggtgcacaa cgcccaggcc cctcgccccg
    12961 ccgacgccct gcaggtggtg aaggagctcc accactgctg tcactggctg gcgcgcacct
    13021 acgcctccgc cgcccgaaga aacgccccgg cgtggagcga cgatctggtg ccttcggttc
    13081 ccgtccccgg ggagctcatc cctcgtcagg agctcgaaat ccgggaaaag gagctcgcgg
    13141 agcaaaacaa ggcgctgctg aaatcccaaa aggagcgcga cgaactggac gcggagatcc
    13201 aggccctgcg ccaccagctc gccgaagccc gcgctgcggc ggaacagcgt ccggacgacc
    13261 acgactactc cgaagcggaa acccgccgcg ccctcatcga cgtggagctc gcccgcgccg
    13321 ggtggcccct ggaagagcgg cgaaaccggg agtaccgggt ggccatgccc aaaaacacca
    13381 cggggagcgc ggactacgtg ctctggggcg acgacaacaa ccctctggca gtggtggagg
    13441 ccaagcgcac caccgtcgat cccctccggg gacaacagca ggccaagctc tacgccgatg
    13501 gtctggaagc gacctacggc atccgccccc tcatcttctg caccaacggc tacaaaacgt
    13561 ggctgtggga cgaccggatc tatcctcccc gggaggtggc ggggtttttc aagaaggacg
    13621 aactgtcccg cctcgccctc cggcgtaccc agcgccgccc cctggacgtg gcccaggtga
    13681 ggcccgccat cgtggatcgg tactaccaga agcgggccat cggcagcatc ttcacgcagt
    13741 tcaccgcggc ccggcggaag gcgctgttgg tgatggccac ggggacggga aagacccgga
    13801 cggccgtcgc cctggtggac gtgctgcagc gggccgggtg ggtgaagcgg gtcctctttc
    13861 tggcggaccg ggtggcgctg gtccatcaag cggtgggggc cttcaagaag caccttcccg
    13921 agagcagccc ggtgaatctt gtcacggaac gagacgccga ggggcgggtc ttcgcgtgca
    13981 cgtaccccac catgatgggg ctcatcgacg agacggaggg gcttcaggcc cgattcagtc
    14041 cgggacattt cgatctggtg atcatcgacg aagcccaccg gtcggtctac cagaagtacg
    14101 gggcgatctt ccgctatttc gacgccctgc tggtggggct caccgccacg ccccgggagg
    14161 acgtggacaa aaacacctac gacctcttcg agctggaaac gggggtgccc acggacgcct
    14221 acgagctggc ccaggcggtg cgcgacggct atctggtgcc cccccggggg gagcaggtgg
    14281 atctgaagtt tccccgggag gggatcacgt acgacgggct ttccgacgag gagaaggccc
    14341 agtgggagaa cctggactgg ggggatgagg accatgccgt cgctcccccc gaccgggtgg
    14401 acccggcggc catcaaccgc tggctcttca accgggacac ggtggacaag atgctccggc
    14461 acctgatgga acacgggcac aaggtggcgg ggggggaccg gatcgccaag accatcgttt
    14521 tcgcccggaa ccaccagcac gcccagttca tcgaagcccg tttcaaccac cactaccccc
    14581 agtacgcggg gcacttcgcc cgggtcatcg acaattccgt ctcgcacgcc cagagtctca
    14641 tcgacgattt ttcccaggca gacaaggcgc cgcacatcgc cctttcggtg gacatgctcg
    14701 acacggggat cgacattccc gaggtggcga atctggtgtt cttcaagccc gtctcctcga
    14761 agatcaagtt ctggcagatg atcgggcgcg gcactcggct ctgccccgag ctcttcggcc
    14821 cgggggacga caagcgggac ttccggatct tcgacttctg cgccaatttc gacttcttcc
    14881 gggagcaccc cgaccagatc gccccagcgg gggatcccgc tccgctgggg gcgcggctct
    14941 tccgggcccg ggtacggctg ctgggatacg tgcagggcac tccggcgctg aacgccgacg
    15001 gcacgctggg aaaggccctc gccgacgggc tccatgccga ggtggcggcc atgaaccggg
    15061 aacatttcgc cgtccggctg gagctcgccg cagtggagcg tttccagcgc cgcgaggctt
    15121 gggaacatct caccgccccg gacagggagg tgctggagcg ccaggtggca aatctcccca
    15181 ccacccttcc cctggaagac gaggccccgc gccgcttcga cctcataacg ctggaaatgc
    15241 agctggccct ggtggaggga gatctgggcc tctttgaaag ccggcgacgg cgggtggtga
    15301 agatggcggc gttgctggaa gaaaaggtcg ccattcccgc agtggccgcc cagcgggcct
    15361 atctgggggc catgcaggag agctccttct gggatggccc gtccatcgcc gggcttgagg
    15421 acatgcgggg acggctccgg gcgttgacgt cctttctcga tcgccggatg cgtcccgtgg
    15481 tctacaccga ttttaacgac gcattccagg gggtgcggga cgtggaggcg gtggccatgc
    15541 ccaaaatgac cggggcccag tacgaaaaaa aggtgaacga cttcctgcgc catcatcaag
    15601 accatctggt gatccaccgc ctgcggaccc accagcccct cacgccccag gacctgcacg
    15661 ggctggagga aatcctggcg tcgctggggg aagaagacgg ccccgggctg ctttcggggc
    15721 ttttggcgaa aagcggcgcc ccgtcggtgg cgcacttcgt ccgaagtctg gtgggcatgg
    15781 atcgggccac ggcccagggg gcgttttcgc agttcctgaa cgaccggagc ctcaccgccc
    15841 cccagatccg tttcattgag atgatcgtgg accaactcac gtcccggggc atcgtggggg
    15901 cttcggcgct ctacgaggcc c
//
LOCUS       DAOOER010000041        15771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_4269_length_15771_cov_9.818147, whole genome shotgun sequence.
ACCESSION   DAOOER010000041 DAOOER010000000
VERSION     DAOOER010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 15771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15771
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4269_length_15771_cov_9.818147"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1592)
                     /gene="htpG"
                     /locus_tag="PL162_06495"
     CDS             complement(<1..1592)
                     /gene="htpG"
                     /locus_tag="PL162_06495"
                     /inference="COORDINATES: protein motif:HMM:NF003555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HQF91705.1"
                     /translation="MAERETFAFQSEGKELLELMVHSVYSNKDIFLRELISNASDALD
                     KLRLESLTDPELAGDLAGGEIRLIVDRERRSLTVADTGIGMNREDLVNFLGTIARSGS
                     REFVRLARERADGVSPELIGQFGVGFYASFMVAERVDVVSRKIRETEAWKWSSSGDGT
                     YAIEEGERDRPGTTVTLFLKPADEEGGMGDYADPHVLRGIVKRYSDFVPWPIRLRGKE
                     EDETLNSMQAIWTRPEGEVTEEEFEEFYHHVSHDWGKPGYRMTTRAEGTTSYRALLFI
                     PERAPLDLFIREGKRGIALYIRRVFIMHDCTELIPEYLRFLRGVVDSEDLPLNISREI
                     LQKDRASMAIRSGLVRKVLETLRTSLAKDREKYRSFWKEFGAVLKEGIFSDEKNRTTL
                     LGVSLFSSSTEADPVTLEEYVGRMKPDQHAIWYLTGSSVEVIKGSPHLEVLAARGYEV
                     LLLSDPVDELWTQGVTEFQGKPLRSAAKGDPEVGGGEDRERAQEARAAAAGEFAALCR
                     VLQGFLDKDVKEVRVSERLTSSP"
     gene            1834..2529
                     /locus_tag="PL162_06500"
     CDS             1834..2529
                     /locus_tag="PL162_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HQF91706.1"
                     /translation="MRQRKILGVVLATMLGLLVTGEGARAMSPTERIRESASILREMA
                     RQDDARYMGKLLKDAKGVAIFPSVVKAGLVFGGKYGEGILLRRDPAKNRWYGPSFIDM
                     GGVSWGLQIGISSTALVLVITNERGMAGFTGDEVTLGGDLAVAAGPTGRSTSAATDGR
                     LKASIYSYSMTKGLFAGLSLEGAVIDTRQDKNREYWKGDRGVDRILKTAATDARMRAL
                     IKALDEVVRTAPK"
     gene            complement(2624..3955)
                     /locus_tag="PL162_06505"
     CDS             complement(2624..3955)
                     /locus_tag="PL162_06505"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQF91707.1"
                     /translation="MDRRWALVQGTLADGEGFFLEDGGILVRGGDIEAVGPSEEIRRR
                     CEEDGTEVISVGGRPVFPGFLNAHHHLYSSLTAGIAPRGAVTDFISNLELLWWHLDGV
                     HDEESVYWSALWGLAQGIRAGVTAVIDHHASQSFVRGSLAMEARAFRDAGVRGVLCFE
                     TSDRGPGTVEDQLEENIDFFRSVASSDDLRGVMGLHASLTLGEESLRRVARRRPPSMP
                     VHVHCGEDRSDRERCRDWGYLGPVDRLARFGLLDQSSLLVHCLHLDEAEYPLLEELGC
                     WVLANPESNAKNGVGRLDQHRLSRWVLGTDGMTGNMLASLRSAFLLGDPGGDALGLLG
                     EAFFVRGHELARQFFPGVGRLVPGAPADLAVVDYSPVTPLTRENLMGHLVFGAQGAEA
                     FITAVRGKILWREGRFTVFDEKELRRGAREAATRLWRRFEERAPGGYRSSL"
     gene            4182..7205
                     /locus_tag="PL162_06510"
     CDS             4182..7205
                     /locus_tag="PL162_06510"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HQF91708.1"
                     /translation="MDEELLATFLAEAAAFLEETEEGLLALEDGSQDPEIIHTIFRHM
                     HSLKGSAGYFGFDSLVRVAHTMESVLDELRRGTKALSPSVTEGLIFATDFLRRTVAHP
                     EGISSSDPSPALSALGGALEPAEPDASGDGESAAAAEEEGLSPIARARGQGRAFFALA
                     LAHLTDLGPLGISQMEFLKRLDQVGNVLSVEEDDPEVATLPGLSPDDLILATEKSASE
                     VAQGLGVPAAWVRPIGTPPAPEPSTPAPRAPEPSAAGAPPSQGSLPGQEVEPPSKTPR
                     EDSVRIRVRLLDDLLNLAGEMVLARNALFREMGRGAEDPRKKIKGLSLRPLPPQGDDP
                     EEGISFRERLRRRSAGMDRALGRIDLLTTEFREKVMQARLQPLDRLFSRLPRLVRDLS
                     RTLGKSVRLALEGGDVELDRSLLEALGDPLIHLVRNALDHGLEFPDERIAAGKAPTGT
                     LTLKAWSRGGRVHLDVFDDGRGLDLDRIRARALERGLASDREIRQMVPDQISRFIFHP
                     GFSTAASISAISGRGVGMDVVLHNVESLGGSVDLHSSAGEGTSFRISLPLTLAVLPSL
                     LVSCGPHHFALPQAHVDEALRLRPAEQLRALSPLGDARVLRLRNSVIPAVVLQELLHQ
                     VSEAPSLSVGPSETSSPPEGRRPAPLPPTPHWLVLRTGDRLFALGVDRILGSEEILVK
                     PLPPRLKGCRIYGGATVLGDERIALILDPEGIAHVTRVGRTQKELDQSALRPWSLPQR
                     EERDLLLVRGAGGDRFALDLSLIARIDEIPPDAIQHAGGMTFASLRGTPVRLLPLEDF
                     MGGGDAPAPSEKRFALTLRFAQGPVAILVHAVLDTLSLPMPSLAPNYPAPGAFGTFDA
                     QGHLVIALDPFVLLARARPPVRPIPKNLLHRRILLLDDDPFGSLLEQSFLQGAGGCEV
                     LRAEDLDGARSALEGADFHALLVTPRLATPEALPRLRRPDGSLPPSALLTGSPRGAPP
                     PGYDGELSRDDREALLKGLKRLCAAGSEAPADGKD"
     gene            7243..7680
                     /locus_tag="PL162_06515"
     CDS             7243..7680
                     /locus_tag="PL162_06515"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HQF91709.1"
                     /translation="MTGKILCFELGTRTVGVDLEEVQEVSRAMAWTSVPGAPREIRGI
                     FNLRGQVAALFDLARTLDPQAPSTEGRSAIILKPSKEGDISGFLVDRLGNVLEVGEGS
                     CSPVPSHLSPREQAAFRELVITGEQVLPVLAPERIFGKDLQDA"
     gene            7716..9407
                     /locus_tag="PL162_06520"
     CDS             7716..9407
                     /locus_tag="PL162_06520"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQF91710.1"
                     /translation="MRNLHAKITMVVGLAGAVACGGVAAIAWHAAQPIAALIPPESAA
                     PLMAQFRHQIALGAALGTALGVAAAVLVAGSINRPVERLLSRILRFGRGDLDVTFLSS
                     LDRNAPLEPPPPENLPFALTGADRLRIMALALEHMGSSLRQSLASVADASERIGASAG
                     ALSLVSQTMVAGSQEINATIFDVNDAVGLIAARLGEMAQSAEEASTGIHNIANALDDL
                     NSSTESTASASEEMSAEVESILETVQDMSCNIDTISTSAIEVSQGVTRMAESVKAIDA
                     ALAETSRNTTRSTRITAEADRQAQETHRLIGRLSSASRQIGKVVDLITDIAEQTNMLA
                     LNAAIQAAGAGEAGRGFAVVANEVKELARQTAEATEEIEGQIEAMREDMGAAVGAVGA
                     IVGVIREVDEISNTIAAAVAQQFQASSQISQVVDVADARMRTITNEIADFSHRFQNVY
                     QSIEEISRAAQAVAHATVTISLASKHAAENADLASTRTAALAQATRDVDHQAKDISAS
                     MENIHHASAETARCAEDTSVSASTLDELAGELHNMMEHFFHSPNAKPALPEAGNE"
     gene            9506..9757
                     /locus_tag="PL162_06525"
     CDS             9506..9757
                     /locus_tag="PL162_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91711.1"
                     /translation="MEGNILVFFNGPWDGDRPEETRRNETSVPRLYRLLSEDPRQRSC
                     YLPRVGTGSLEGAAALYDFDPHDPPQRRGRAPQDPGFSL"
     gene            complement(9966..10712)
                     /locus_tag="PL162_06530"
     CDS             complement(9966..10712)
                     /locus_tag="PL162_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HQF91712.1"
                     /translation="MGKIVSMFLGFFLLLVVPMGATRAFSAPGREELLERPAAGVIVG
                     RHVNLRDRPSTEGRSLGRWGDGEQEELVVVDAARGEDGQTWYQVLSERLGEGWVHGRF
                     LRFEGLDDPLGRFCMMLRRDFGTEPSMTERRLGKPEGVDRRTFHPEDYPGEVTAVELR
                     YPGGRVIFWITEKGSFLTEVDFEKGVSFGSIAFGDAPEKVSRLIGDPHEVDHRVWTYH
                     SGMRRVIVGFGFDPEGDPVVNRMIFERDPY"
     gene            complement(10834..12426)
                     /locus_tag="PL162_06535"
     CDS             complement(10834..12426)
                     /locus_tag="PL162_06535"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQF91713.1"
                     /translation="MGPFKDRLEVANRLIGEADFRSALRLLHGVLEDAEARGDDYATA
                     VILNRLGRAYRGLGNPALSIQYHMKALDIFQRMEDHRGLADTYNSLGVAWSRGGDRAE
                     TVKFRLKGLEAAQHSGDLRELSRAYNNVGEIYREEGRCAEAQEHYLRSMEFDRQAGNK
                     AYEAVGLHNLALLAMAEGDIPEARRFFRESLVLSAEARDKEMEAETLVRWGTLEMQAH
                     RWHEAEDCLKRGLALAETLQSSQLLGVALGEMAAYHRARGNYLEALGALERLTEISRE
                     EYRNIQASRLLAVPVEMEVERSREKSQELAHENKALRELIAEQVREIDLDKEEALNLR
                     DRAQKTLEDSLLALASVIEAKDPHTGGHGARVAGYARALARSLGLFPGDRQRIYLGAI
                     VHDVGKIGVPDAILGKPNSLERWEQETLQRHPTMGHQILSRIQGLDAAAEVALCHHER
                     WDGKGYPRGLKGTDIPLFARIVAIANVWDNLVSSRPHRSVLTREEARRAMTAERGGAF
                     DPDLLDLFLDEKEGISRLYRPW"
     gene            complement(12486..14087)
                     /locus_tag="PL162_06540"
     CDS             complement(12486..14087)
                     /locus_tag="PL162_06540"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQF91714.1"
                     /translation="MEEDHRGKDLFAAADQLIRAADFQGAIHLLHEILAESERERRPI
                     DVAKAFNRIGRSHASLGNADLAIRYHLRALTIFEKAEDLRGLAETYNHIGTIWSEAEN
                     NSMAMQFKLKALDAAQRSEDLRELSRAYNNIGEEYRLSGRLDDARDHYLRSLELDRQA
                     GNKAYEAVALHNLALVASAEDNYGEARDLFEKSLILSLEAGDREMEVETLIFFGEMES
                     RFHRFDSAGFLLDRALTRAEGLGSPYLLGLALREMAAYLRGKGDFPRAFDAMDRFTKV
                     TRGESSAAQRKRFLSIFAEMEADRTREESRRLESEAGELKELIAHQVREIESDKEEAL
                     RLRDQAHKTLEELLLAMASAIEAKDLCTGDHVERVGGYARALARCAGLPQEECQRIYL
                     GAIVHDVGKIGVRDAVLNKPGILDPEEREEMRRHSALGRQILSQVRGLEIAADIAHCH
                     QERWDGGGYPRGLRGTEIPLSARIVTIADVWDAISSHRPYRPAMSREKAMELMIYERG
                     RAFDPDLLDLFIGDEHRIYELYDRG"
     gene            complement(14594..>15771)
                     /locus_tag="PL162_06545"
     CDS             complement(14594..>15771)
                     /locus_tag="PL162_06545"
                     /inference="COORDINATES: protein motif:HMM:NF037272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lectin like domain-containing protein"
                     /protein_id="HQF91715.1"
                     /translation="NNGGDDWRAVAILARGTGAVDEDDAPYMGSGWDTPDPSGEEPRR
                     VLLTEALYLPYQDGAFEYPLLHRAIKTSVMTYGASSIGMFAEFGASYYNPATFAYHYA
                     GPLNSNHAVCIVGWDDAYPKENFPAGHRPAEDGAWIVRNSWGSSFGDGGYFYASYAST
                     LDSGVNYIGVPEVSAAAQVAGGQERIHDYDPLGWCNTYGFDSETGWFANVFPLSEDAM
                     LKAVSFYVAAESASYQVRVYSDVSPDASGGFRGTLVTGPLVGSLDIPGYRRVAMSGDV
                     VLPGGKNFAVEVRLTTPGYLFPVPLELPLEGYSDKATAQPRQSWVSANGAAWEDLTTD
                     VPNANVCLKAFTVPYVAPTPTPAPTSSPSSASGGGCSTGLVPGALLLLVPLAFFLRR"
BASE COUNT         2781 a         5409 c         4802 g         2779 t
ORIGIN      
        1 ggagaggagg tgaggcgctc ggacacccgc acttctttca cgtccttgtc caaaaatccc
       61 tggaggaccc ggcaaagggc cgcaaattcc ccggcggcag cggctcgggc ctcctgggcc
      121 ctttcccgat cctcgccccc gcccacctcc ggatccccct tggcggcgga gcgtaggggc
      181 ttgccctgga actccgtgac gccttgggtc cagagctcgt ccacggggtc ggacaaaagc
      241 agcacttcgt atccccgggc cgccagcact tccaggtgag gagatccctt gatcacctcc
      301 accgaagagc ccgtgaggta ccagatggcg tgttgatcgg gcttcatacg ccccacatac
      361 tcttccagcg tcacggggtc ggcctccgtg gaagaggaaa aaagagaaac ccccaggaga
      421 gtcgtccggt tcttctcatc ggaaaagatc ccttccttga gcaccgcgcc gaattccttc
      481 cagaagctcc ggtacttctc ccggtccttg gccagggagg tgcgcagggt ctccaggacc
      541 ttgcgcacca ggccggagcg gatagccatg gaggcccggt ccttctgaag gatctcccgg
      601 gagatgttta agggcaaatc ctcggaatcc accacgcccc ggaggaaccg gagatactcg
      661 gggatgagct cggtgcagtc gtgcatgatg aacacccggc ggatgtagag ggcgatgccc
      721 cgtttgccct ctcggatgaa gaggtccagg ggggcccgct cggggatgaa aagcagggcc
      781 cggtaactcg tggtgccctc cgcccgggtg gtcatgcggt aaccgggctt gccccagtca
      841 tggctcacgt ggtggtagaa ctcctcaaac tcttcctcgg tgacttcgcc ctcgggacgg
      901 gtccagatgg cctgcatgga gttgagcgtc tcgtcctctt cctttccccg caggcggatg
      961 ggccagggga cgaagtccga gtagcgtttg acgatgcccc gtagcacgtg ggggtccgca
     1021 tagtctccca tgcccccctc ttcgtcggcg ggtttcagaa aaagcgtcac cgtcgtgccg
     1081 gggcggtccc gttccccttc ttcaatggca taggtgccat ccccggagga ggaccacttc
     1141 caggcctccg tctcccggat cttccggctc accacatcta cccgttccgc caccatgaag
     1201 ctagcataaa atcccacgcc aaactggccg atgagctccg gagacacccc gtcggcccgc
     1261 tctcgggcga ggcgcacgaa ctcccggcta cccgagcggg cgatggtgcc cagaaagttc
     1321 accaggtctt ctcggttcat gccaatgccc gtatcggcca cagtgaggct ccgacgctcc
     1381 cgatccacga tgagccggat ctctcctccg gcgaggtctc ccgccagctc gggatcggtg
     1441 aggctctcca ggcgaagctt atccagggcg tccgaggcgt tggagatcaa ctctcggagg
     1501 aagatgtcct tattggagta caccgagtga accatgagct ccaggagctc cttgccctcg
     1561 ctctgaaagg caaatgtttc ccgttccgcc atccttcttt ctccctccca tgccgtagcg
     1621 gcgcccctct ttaacggggc tccgccgtca actcccccca attataaggc acccccggca
     1681 aagcttccat gacgaaaatc cccggacatt tccttttgcc ccctctaggt taggaatctc
     1741 ttcccgggac gcccgttgca aaaagcgatc cgaacgggga aaatgggagt agcgccgggg
     1801 ataaagccct ggggcacgaa aggagacggc accgtgagac agagaaagat cttgggggta
     1861 gttttggcga cgatgttggg tcttttggta acgggggagg gggcccgggc catgtccccc
     1921 acggagcgca tccgcgagag tgcctccatc ctgcgggaga tggcccgtca ggacgacgcc
     1981 cggtacatgg gaaagttgct taaagacgcc aagggagtgg ccatcttccc ctccgtggta
     2041 aaggctgggt tagtcttcgg gggaaagtac ggcgagggca tcctgctgcg ccgggacccg
     2101 gccaagaacc ggtggtacgg ccccagcttc atcgacatgg gaggggtctc ctgggggctc
     2161 cagatcggca tctcctccac cgccctggtg ctggtcatca ccaacgagcg cggcatggcg
     2221 ggtttcaccg gggacgaggt caccctggga ggggatctgg cggtggccgc cgggcccacg
     2281 gggcggagca cctccgccgc cacggacggc cgcctcaagg cgtcgatcta cagttactcc
     2341 atgaccaagg gactcttcgc cggcctatcc ctagaagggg ccgtcatcga cacccgccag
     2401 gacaaaaacc gggaatactg gaagggcgac cggggggtgg accggatcct gaagaccgcc
     2461 gccaccgatg cccggatgag ggccctcatc aaggccctgg acgaagtggt ccgcaccgcg
     2521 ccaaagtaac aagctccctc gccccgaaaa aaggggtcct cagggtcccg gggcctttcc
     2581 cccggacccc tgaggacccc acccgaaatc ccgaaagccc cgcctagagg gaggagcggt
     2641 accctccggg ggccctttcc tcaaagcgcc gccatagcct ggtcgccgct tcccgggcgc
     2701 ccctgcggag ctccttttcg tcgaagaccg taaatcttcc ctcccgccac aggatctttc
     2761 ccctcaccgc cgtgataaag gcctcggccc cctgggcccc gaagaccaag tgtcccatga
     2821 ggttttcccg ggtcagggga gtcacggggc tatagtccac caccgccagg tcggccggag
     2881 ccccgggaac caggcgcccc actccgggga aaaactgccg cgccagttcg tgtccccgga
     2941 caaagaaggc ttcgccgagg agccccaggg cgtccccccc gggatctccc aagagaaagg
     3001 ccgaacgcag cgaggccagc atgtttcccg tcataccgtc ggtgcccaac acccatcgag
     3061 agaggcgatg ctggtccagg cgccccacgc cgtttttggc gttggactcg gggttggcca
     3121 gcacccaaca tcctagctcc tccagcaggg ggtactccgc ctcgtccaag tgcaagcaat
     3181 gcaccagcag ggaggattga tccagcaggc caaaacgggc caggcgatcc acggggccca
     3241 gatagcccca gtcccgacat cgctcccggt ccgatcgatc ctcgccgcag tggacgtgca
     3301 ccggcatgga gggggggcgg cgccgggcca cccggcgcag gctctcctcc cccagggtta
     3361 gggaggcgtg gagccccatc acccctcgaa ggtcgtccga gctcgccacc gaccggaaaa
     3421 agtcgatgtt ctcttccagc tgatcttcca ccgtccccgg cccccggtcc gaggtctcaa
     3481 aacacaggac cccccgcacc cccgcgtccc ggaatgcccg ggcctccatg gccagactcc
     3541 cccggacaaa ggattgggag gcgtggtgat cgatcacggc ggtcaccccg gctcggatcc
     3601 cctgagccaa accccagagg gcggaccagt acaccgattc ctcgtcgtgg accccatcca
     3661 gatgccacca gaggagctcc aggttggaga taaagtccgt caccgcccct cggggggcga
     3721 ttcccgccgt cagggaggag tatagatggt gatgggcatt taaaaacccc gggaacaccg
     3781 gacgcccccc caccgatatc acctccgtcc catcctcttc gcaccggcga cggatctcct
     3841 ccgaaggccc caccgcctct atgtcccccc ctcgcaccag gatccctccg tcttcgagaa
     3901 aaaagccctc tccgtccgcc aaggtgcctt gcaccagggc ccagcgtcga tccacctcaa
     3961 acccctcccc cttcggtctt tacccttccg cccgcagttt acccctccgg aggacctttt
     4021 gtccccccta ccttcatttg ccccgcccct tggcccatcc ttggagggaa tgattcggcc
     4081 ccttttccac tatgtggtag aatctttcta ggctcgggcc cttcctgcgc ggggcgcgag
     4141 tgaagtgtgc cctcttttcg gaaggaggct ccggaccgat catggatgag gaactgctgg
     4201 cgaccttcct cgccgaagcc gccgccttcc tggaagagac ggaagagggg ctcctggccc
     4261 tggaagatgg gagccaagac cccgagatta tccacaccat cttccgtcac atgcacagcc
     4321 tgaagggatc ggcgggatac ttcggcttcg actccctggt tcgggtggcc cataccatgg
     4381 aaagcgtact ggacgagctc cgccggggca ccaaggcctt atctccgtcg gtgactgagg
     4441 gattgatttt tgccacggat tttttgcgcc gcaccgtggc ccaccccgaa gggatctcgt
     4501 cctcggaccc ctctccggcc ctttccgccc tcggcggggc ccttgaaccc gcagagccgg
     4561 acgcctcagg agacggagag agcgccgccg ccgccgaaga agaaggtctt tcccccatcg
     4621 cccgggcccg gggccaaggg cgggcctttt ttgccctcgc cctggcccat ctcaccgacc
     4681 tgggccccct ggggatctcc cagatggagt ttctgaagcg ccttgaccag gtggggaacg
     4741 tgctctccgt cgaagaggac gaccccgagg tggccaccct tcccggcctc tccccggacg
     4801 acctgatcct tgccaccgaa aaatcggcgt cggaagtcgc ccaggggcta ggggtccccg
     4861 ctgcgtgggt gcgccccatc ggcactcccc cagccccgga gccctccact cccgcccctc
     4921 gggccccgga accctccgcc gccggagctc cgccctccca aggctccctt cccgggcagg
     4981 aggtcgagcc cccatcaaag acccctcggg aggactcggt gcgcatccgg gttcgcttgc
     5041 tggacgactt gctgaacctg gcgggagaga tggtgttggc ccgcaacgct cttttccggg
     5101 agatggggcg gggcgccgaa gaccctcgca agaaaatcaa aggcctttcc cttcgccctc
     5161 tcccgcccca gggagacgat cccgaggaag gaatctcctt ccgagagagg ctccggcgcc
     5221 ggtcggcggg catggatcga gccctgggac gcatcgacct cctcaccacg gagtttcgag
     5281 aaaaggtcat gcaggcccgg ctccaacccc tggatcgcct cttttctcgc ctgccccgcc
     5341 tggtgcggga cctatcccgc acgctgggaa aaagcgtccg cctggccctg gaggggggcg
     5401 atgtggagct ggatcggtcg ctgctggaag ccctgggaga tcccttgata cacctggtgc
     5461 gaaacgccct ggatcacggc ctggaattcc ccgacgaacg tatcgccgca ggtaaggccc
     5521 ccacgggaac cctcactttg aaggcctggt cccggggggg gcgggtgcac ctggacgtct
     5581 tcgacgacgg ccggggactg gacctggatc gcatccgggc ccgggccctg gagcgcggtc
     5641 tagccagcga tcgggagatc cgccagatgg tccccgacca gatctcccga tttatatttc
     5701 atccaggatt ttccaccgcc gcatccatct cggccatctc cgggcgaggg gtggggatgg
     5761 acgtggtact tcacaacgtg gagagcctgg ggggcagcgt ggacctgcac agctccgccg
     5821 gagaagggac ctcctttcgt atcagcctgc ccctcacctt ggcggtgctc ccttcgctgt
     5881 tggtgtcctg cggccctcac cacttcgccc tcccccaggc ccacgtggat gaggccctgc
     5941 gcctgcgccc ggcggaacag ctccgggccc tctctcccct gggagatgcc cgggtcctga
     6001 ggctccgaaa cagcgtgatc cccgccgtgg tgctccagga gctcctgcac caggtctcgg
     6061 aggccccgtc gctgtccgtc ggcccttccg agacctcgtc cccaccggaa ggaagacgtc
     6121 ccgcccccct cccccccacg ccccactggc tggtgcttcg caccggggac cgtctcttcg
     6181 ccctgggggt ggatcgcatc ctgggcagcg aggaaatcct ggtgaaaccc ctccctcctc
     6241 gcctgaaggg gtgccggatc tacggcggcg ccaccgtcct gggggacgag cggatcgccc
     6301 tcatcctgga ccccgagggc atcgcccacg tgacccgggt ggggcgcacc cagaaggagt
     6361 tagaccagag cgccctccgg ccctggtccc ttccccagcg cgaagagcgc gatctgctcc
     6421 tggtgcgggg ggcggggggc gaccgctttg ccctggacct atcgctcatt gcacgaatcg
     6481 acgagatccc cccggacgcc atccagcacg cagggggcat gaccttcgcc tccctccggg
     6541 ggacgccggt gcgcctgctt cccctggagg acttcatggg gggcggcgac gccccggcgc
     6601 cctcggaaaa gcgcttcgcc ctgacccttc gttttgccca gggccctgtg gcgatcctgg
     6661 tgcacgccgt cctggacacc ctgagcctcc ccatgccttc cctggcgccg aactatcctg
     6721 cccccggggc ctttggcacc ttcgacgccc agggacacct ggtgatagcc ctggatccct
     6781 tcgtgctcct ggcccgggct cgccctccgg tacgccccat cccgaaaaac ctgctccatc
     6841 gccgaatatt gctcctggac gacgatccct tcggatccct actggagcag agcttcctcc
     6901 agggcgcagg ggggtgcgaa gtccttcggg cggaggacct ggacggggct cgctccgccc
     6961 tggaaggcgc cgattttcac gcccttctgg tcacccctcg tctggccacc ccggaggctc
     7021 tcccccggct gcgccgcccc gatgggtcgc tcccgccttc ggccctgctg accggatcac
     7081 cccggggagc ccccccgccg ggatacgacg gagagctctc ccgggacgac cgggaggccc
     7141 tgctaaaggg actaaagcgg ctctgtgctg cgggcagcga ggcgcctgct gatggcaaag
     7201 actgaaccga caaagcggct aaacttaagg aggcccttcg acatgacagg aaagatcctc
     7261 tgtttcgagc tgggaacgcg cacggtgggc gttgatctgg aggaagtgca ggaagtatcg
     7321 cgcgccatgg cttggacctc ggttcccggg gctccccggg agatccgggg catcttcaac
     7381 ctgcgggggc aagtggcggc cctctttgac ctcgcccgaa ccctggatcc ccaggcccct
     7441 tccaccgaag gacggtcggc catcatccta aaacctagca aggaagggga tatctcgggc
     7501 tttctcgtgg accgtctggg caacgtgttg gaagtgggag agggctcctg ctcccccgtg
     7561 ccttcccacc tctctccccg ggagcaggcg gcgtttcggg agctggtcat caccggggaa
     7621 caggtcctcc ccgtattggc gccggaacgg atcttcggca aagacctcca agacgcgtaa
     7681 aaggtccgct agctcttaga gagagaggga tggtcatgcg aaacctgcac gctaagatca
     7741 ccatggtggt gggcctcgcc ggggccgtgg cctgcggtgg cgtcgccgcc atcgcctggc
     7801 acgccgccca gcctatcgcc gccctgatac cccccgaatc agccgccccc ctgatggccc
     7861 aattccgcca ccagatcgcc ctgggggcgg ccctggggac ggctttggga gtcgccgccg
     7921 ccgtactggt ggcgggcagc atcaaccgcc ccgtggagcg cctcctgtcc cggattttgc
     7981 gctttggccg gggggatctg gatgtcacct tcttgtccag cctggaccga aacgcccccc
     8041 tggagccccc tcccccggag aacctcccct ttgccctgac cggtgccgac cggcttagga
     8101 tcatggccct ggccctggag cacatgggct cttccctacg ccaatccttg gcgtccgtgg
     8161 ccgacgcctc ggagcgcatc ggggcatcgg cgggagccct ttccctcgtg tcccagacca
     8221 tggtggcggg aagccaggag atcaacgcca ccatcttcga cgtcaacgac gccgtggggc
     8281 tcatcgccgc ccgcctgggg gagatggccc agagcgccga agaggccagc acgggcatcc
     8341 acaacatcgc caacgccctg gatgacctaa attcctccac ggagagcacc gcctcggcct
     8401 cggaggagat gtccgccgag gtggaaagca ttctggagac ggtgcaggac atgtcgtgca
     8461 acatcgacac tatttcgacc tccgccatcg aggtgtccca gggggtcacc cgcatggccg
     8521 agagcgtcaa ggccatcgat gccgccctgg cggagacatc ccggaacacc acgcggtcca
     8581 cccgcatcac cgccgaggcc gaccgccagg cccaggagac ccaccgcctc atcgggcgcc
     8641 tctcctccgc atcccgccag atcggcaagg tggtggacct catcaccgac atcgccgaac
     8701 agaccaacat gctcgccctc aacgccgcca tccaggccgc cggagcgggg gaagcggggc
     8761 ggggcttcgc cgtggtggcc aacgaggtca aagagctggc gcgccagacc gccgaagcca
     8821 cggaagaaat cgaagggcag atcgaggcca tgagagaaga catgggcgcc gccgtgggcg
     8881 ccgtgggggc catcgtgggc gtcatccggg aagtcgacga gatcagcaac accatcgccg
     8941 ccgccgtcgc ccagcaattc caagcctcct ctcagatctc ccaggtggtg gacgtggcgg
     9001 acgctcgcat gcgcaccatc accaacgaga tcgccgactt ctcgcaccga ttccaaaacg
     9061 tgtaccagag catcgaagag atcagccggg ccgcccaggc cgtggcccac gccaccgtca
     9121 ccatatccct ggcctccaag catgcggcgg aaaacgccga tctcgccagc acgcgtaccg
     9181 ccgccctggc ccaggccacc cgggatgtgg accaccaggc caaagacatc tccgccagca
     9241 tggaaaatat ccatcacgcc tctgcagaaa ccgcccggtg cgccgaagac accagcgtct
     9301 ccgcctccac cctcgacgag ctggccggag agctgcacaa catgatggaa catttttttc
     9361 attcccccaa cgccaaacct gcccttcccg aagcggggaa cgaataaaaa aatctctccg
     9421 aggctggact gttctttgcc acaccctaca atggcgtcgt ggcgcttcca cacatgaagt
     9481 gcccccttcg ggaggtgagg ccatcatgga aggaaatatt ttggtctttt ttaacggccc
     9541 atgggacggc gaccgccccg aagagacgag acgcaacgaa accagtgtgc cccggcttta
     9601 tcgccttctt tccgaagatc cgcgccagcg gtcgtgctat ctccccagag tgggtaccgg
     9661 ttctctggaa ggggccgcag ccctctacga cttcgacccc cacgatccgc cccagcgacg
     9721 gggaagagct ccgcaagatc cgggattttc tctttgaaaa cggactgctg acggaaaagc
     9781 ccggggtgga aacactgctg gaaaagtagg ggcactccgg aaatccccac agcacaggcc
     9841 ccgggctcgg cgtggtacac cagaaagccc ggggcccttt cgtggggcga gggaggatca
     9901 ggggcggcgg tggttccaaa ggtctccggg ggccccgatc ccggcctgaa gtgccggaga
     9961 gaggttcagt aggggtcgcg ctcgaagatc atgcggttta ccacgggatc accttccgga
    10021 tcaaagccaa aacctacgat aacccgccgc atcccggagt ggtaagtcca aacgcgatgg
    10081 tcgacctcgt gggggtcgcc gatcaagcgc gacaccttct ccggggcgtc gccaaaggca
    10141 atcgatccga aggatacgcc tttttcaaag tccacctccg tcaggaagga tcctttttcc
    10201 gtgatccaaa agatcacgcg ccccccgggg tagcgtagct ccaccgccgt cacctctccg
    10261 gggtagtcct ccgggtgaaa ggtcctgcga tccactccct cgggctttcc tagccgccgc
    10321 tccgtcatcg aaggctccgt cccaaaatcc ctccggagca tcatgcaaaa ccgccctaac
    10381 gggtcgtcca acccctcgaa gcggaggaat cggccgtgca cccaaccctc ccctagcctc
    10441 tccgagagga cctgatacca ggtttggccg tcctctcccc gggccgcatc cacgacgacg
    10501 agctcctctt gttccccatc cccccaccgg ccgaggctcc tgccttcggt cgagggacga
    10561 tcccgaaggt tgacatgacg gccgacgatc actccggcgg cggggcgttc gagcaattct
    10621 tcccgccccg gcgcggaaaa agccctcgtc gcccccatgg ggacgacgag gagcaggaaa
    10681 aaccccagaa acatcgagac gattttcccc aagaagctca tctcttttcc ctatcctttc
    10741 ggtcgatcat gacgccccgg gccgggaggg gtccgctggg ccaagccgaa ctggggtccc
    10801 ctccttccag agacctccct aaagaggacc cggtcaccag gggcgataaa gccgggagat
    10861 gccttctttt tcgtccagga agaggtcgag gaggtccggg tcaaaggccc ctccccgctc
    10921 ggccgtcatg gcccgccggg cctcttcccg agtcaggacc gatcggtgcg ggcgagacga
    10981 caccaggttg tcccagacgt tggcgatggc cacgatgcgg gcaaagaggg ggatatcggt
    11041 gcctttcagc ccccggggat agcccttgcc gtcccatctt tcgtgatggc atagggccac
    11101 ctcggcggcg gcgtccagcc cctggatgcg ggaaaggatc tggtgtccca tggtggggtg
    11161 acgttggagg gtctcttgct cccagcgctc cagggagttt ggtttgccga gtatagcgtc
    11221 ggggacgccg atttttccca cgtcgtggac gatggccccc aggtagatgc gctggcgatc
    11281 tccggggaag agtcccaggc tccgggccag ggcccgggcg tagcccgcca cccgggcgcc
    11341 gtgccctccg gtgtggggat ctttggcctc gatgaccgat gccagggcca ggagagaatc
    11401 ttccagggtc ttctgggccc ggtctcggag attcagggct tcttccttgt cgaggtcgat
    11461 ctctctcacc tgttcggcga tgagctctcg aagggccttg ttctcgtggg ccagctcctg
    11521 ggatttctcc cgggaccgct ccacttccat ttcaacgggt accgccaaaa gcctgctggc
    11581 ctggatgttt cggtactctt ccctggagat ttccgtgagg cgctccaggg cgccgagggc
    11641 ttccaggtag tttcccctgg cccggtggta ggcggccatt tcccccagcg ccacacccag
    11701 aagctgggag gattgcaggg tctccgccag ggcgagaccc cttttcaggc agtcttccgc
    11761 ctcgtgccat cgatgggctt gcatctccag ggtgccccat cggacgaggg tctctgcttc
    11821 catctcctta tccctcgctt cggcgctcag gaccaggctc tcccggaaaa atcttcgggc
    11881 ctcggggatg tctccttccg ccatcgccag aagggcgagg ttgtgcaggc ccaccgcttc
    11941 gtaggctttg tttcccgcct ggcggtcgaa ttccatggac cgaaggtagt gctcctgggc
    12001 ctcagcgcag cgcccctctt cgcgatagat ctctcccacg ttgttatatg cccgggaaag
    12061 ctctcgcagg tcccccgagt gctgggccgc ttcgagcccc ttgagcctga acttaacggt
    12121 ctcggcacgg tctcccccac gagaccaggc cactcccagg gagttgtagg tgtctgccag
    12181 gcctcgatgg tcttccatgc gctggaatat gtcgagggct ttcatgtggt actggatgga
    12241 aagcgccggg tttcccaagc cccggtaggc ccggcccagg cggtttaaaa tcacggcggt
    12301 ggcgtaatcg tcgccccggg cctcggcatc ctccaggacg ccatggagga ggcgcagggc
    12361 gcttcgaaag tcggcctccc cgatcaggcg attggccacc tctagcctgt ctttgaacgg
    12421 tcccatgaat tccatgcctc atgcctcgat tctcggctct ttctaggtga caccccgagg
    12481 agaagtcacc ctcggtcgta gagctcgtag atgcggtgct catcgccgat gaaaagatcc
    12541 aacaggtcgg ggtcaaaggc cctaccccgt tcgtagatca tgagttccat cgccttttct
    12601 cgggacatgg ccggacggta ggggcgatgg gacgagatgg catcccagac gtcggctatg
    12661 gtcacaatgc gggcagacag ggggatctcg gtgccccgga gaccccgggg atagcccccg
    12721 ccgtcccagc gctcctggtg gcaatgggcg atatcggcgg cgatttctaa cccccggacc
    12781 tgggaaagga tctgacgccc cagggcggag tggcgccgca tctcttcccg ttcttcgggg
    12841 tcgaggatcc ccggtttatt gagcacggcg tcgcgcacgc caatcttacc cacgtcgtgg
    12901 actatggccc ctaggtagat ccgctggcac tcttcttggg gcaaccccgc gcaccgggcc
    12961 agggcccggg cgtatccccc caccctctcc acgtggtctc cggtgcagag gtccttggct
    13021 tcgatggccg acgccatggc taggaggagc tcttccaggg tcttatgggc ttggtcccgg
    13081 agcctcaggg cttcttcttt gtcgctttcg atctcccgca cctggtgggc aatgagctct
    13141 ttcagctccc ccgcttcgct ctccaggcgc cgggactctt cccgggtccg gtcggcttcc
    13201 atctcggcga agatcgacaa aaatctcttc cgctgggccg cagaggactc tcccctcgtg
    13261 accttggtga aacgatccat cgcgtcgaaa gcccggggaa agtccccttt cccccggagg
    13321 tacgccgcca tctcccgcag agccaggccc aaaaggtacg gagaccccaa accctcggcc
    13381 cgggtcaggg ccctatccag gaggaagccc gccgagtcaa atcggtggaa ccgggactcc
    13441 atctcgccaa agaagatgag ggtctccact tccatctccc gatctccggc ctccaagcta
    13501 aggatcaggc ttttttcgaa aagatcccgg gcttcgccgt agttgtcctc cgccgaggcc
    13561 accagggcca agttgtgtag ggccacggcc tcgtaggctt tgtttcccgc ctgacggtcc
    13621 agctccaggg agcggaggta gtgatctcgg gcgtcgtcga ggcgaccgct caggcggtat
    13681 tcttcgccga tgttgttgta cgcccgggaa agctctcgca aatcctccga gcgctgggct
    13741 gcgtccaagg ccttgagctt gaactgcatg gccatggagt tgttttccgc ctcggaccaa
    13801 atggtgccaa tgtggttata cgtctccgcc agaccccgga ggtcttcggc cttttcaaag
    13861 atggtcaggg cccgcaggtg atagcgaatg gccaagtcgg cgttccccag gctcgcgtgg
    13921 ctccggccta tgcggttaaa ggccttagct acatcgatgg ggcgccgctc tctttcgctc
    13981 tcggcaagga tctcgtggag cagatggatc gccccctgga aatcggcggc cctaatgagc
    14041 tggtcggcgg cggcgaagag gtcctttccc cggtgatctt cttccacgga tcatacctcc
    14101 tttccatctt ctcgcggcac gcccctccgc agaccatcgg ggaatcgtcg gggccagaaa
    14161 gcccccgcgc cgtgggtgat tcatattcga ggcgatggcg aagggcccct ccgacggagg
    14221 gtccctgacc cttcgtagag tgtagctttt ttttacactt tttgcaccct ttttggtgtg
    14281 gcccctggcg ggggatatcg gaggtcttac ccgtcggggc gaaaggtcgg gggatgatgt
    14341 ggggataaaa agaataaaaa aagccggctg gaagccggcg ctcccggcaa tggcgctccc
    14401 ggcaaaagaa agcgaaagtc gggacaaaag cccccctatt tcccaagaaa agccccggcg
    14461 gagggatctc ctccgccggg aaaaggggct ttattcccgt gccgtgaagg gaagtgcccc
    14521 gcttcggcgc gcccctcggg gtctttgtcc gtgctccgaa agcaggcgct cctcggaggc
    14581 tccccccatc ctcctaccgc ctcaggaaga aggctaaggg cacaagaagc agcaacgccc
    14641 cggggacgag ccccgtggag caccccccac cgctggcaga actgggagaa ctcgtagggg
    14701 ccggcgtggg ggtcggtgcg acatagggta ccgtaaaggc cttgaggcag acgttggcgt
    14761 tgggcacgtc cgtggtgagg tcttcccaag ccgcgccgtt agcgctcacc cagctctggc
    14821 ggggctgggc cgtggccttg tcggaatacc cctccaaagg gagttccagg ggcacgggga
    14881 acaggtaccc cggagtggtg agccgcacct ccaccgcgaa attcttgccc cccggcaaca
    14941 ccacgtcgcc gctcatcgcc acccggcgat acccggggat atccaggctg cccaccaggg
    15001 gccccgtcac cagcgtcccc cggaagcccc ccgaagcatc gggggaaacg tcgctgtaaa
    15061 ccctgacctg atatgaggcc gactccgccg ccacgtagaa cgacacggcc ttcagcatcg
    15121 cgtcctccga aagggggaag acgttggcaa accaccccgt ttcactgtcg aaaccgtagg
    15181 tgttgcacca ccccaggggg tcgtagtcgt gaatgcgctc ctgccctccc gccacctggg
    15241 ccgccgccga aacttcggga actccgatgt aatttacccc gctgtccaag gtggatgcat
    15301 aggaggcgta aaaataaccc ccgtcgccga aggagctccc ccagctgttg cggacgatcc
    15361 acgccccgtc ttcggcggga cggtgccccg ctggaaagtt ctccttcggg taggcgtcgt
    15421 cccaccccac gatgcacacc gcatggttgg agttcagagg accggcgtag tggtaggcga
    15481 aggtggcggg gttgtagtac gacgccccga attcggcgaa catgccgatg gatgaggcgc
    15541 cataggtcat caccgaagtc ttgatggccc gatgaagaag gggatattca aaggctccgt
    15601 cctgatatgg caggtagagg gcctcggtga ggagcacccg ccggggttct tcccccgaag
    15661 ggtcgggggt gtcccatccc gaccccatgt agggggcgtc gtcctcgtcc acggcgccgg
    15721 tgccccgggc caggatggcc acggcgcgcc agtcgtcccc gccgttgttg t
//
LOCUS       DAOOER010000042        15560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_4353_length_15560_cov_9.834763, whole genome shotgun sequence.
ACCESSION   DAOOER010000042 DAOOER010000000
VERSION     DAOOER010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 15560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15560
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4353_length_15560_cov_9.834763"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PL162_06550"
     CDS             <1..514
                     /locus_tag="PL162_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HQF91716.1"
                     /translation="GLFFGAYGLAKVTGHWQTTVSPAANAARGGNPAEGVYGWMTLAQ
                     VAGVVKLPPEKVLAIAGLPADTPQDVSMKKIPGLNDEAVQEALRAHFAASPSGKAQLP
                     SNPDEIRGSVTLEEIAQTFDLDPGKILEAAGWPADSPRDRPIKEVGAALGKEVSEIRA
                     AVAALKKGAK"
     gene            complement(573..648)
                     /locus_tag="PL162_06555"
     tRNA            complement(573..648)
                     /locus_tag="PL162_06555"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(612..614),aa:Glu,seq:ctc)
     gene            complement(667..741)
                     /locus_tag="PL162_06560"
     tRNA            complement(667..741)
                     /locus_tag="PL162_06560"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(706..708),aa:Gln,seq:ctg)
     gene            1037..3418
                     /locus_tag="PL162_06565"
     CDS             1037..3418
                     /locus_tag="PL162_06565"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HQF91717.1"
                     /translation="MTFFSYRRCLALALVGATALVGSSAAFARNQDRYLERHPDAPRA
                     LRLAAPQAIPAVPYGGNNESLSDYPDLVQQWFDAKTEGVTFFTPAFTSNHGSFEQGGV
                     SWGTTQEEMISFLNALPKDYLTMRIIGEVATRDNKGVVEGSFKVPLLVFSKPAAFDPA
                     DLHAKKKPIVYIQAMIHGNETSGCEAVLRLASQLARDDLKVLDKISVVLLPRYNVDGA
                     WKYQRGTDTISPVRVNMDQNRDHTAFESPLTRLMHTVANQYQPHVALDLHEMNYDHTA
                     DYRVSGGDFVRFRDYHYFDLTTLVAHLYNVPAGITTLAREMEAQISADLTARGLAWNF
                     YFYPWDMTQERAEIQIGGLPVAVSRDVITEIMEGPPDEAMGDSVLSLIPSIGMLFETR
                     SPRVLVNYKPRVYAHATALESVINQTASRADTIRNTVESGRAAMANLGKSVSPTNTIT
                     LWIRQGQVSHLSFPVLRMSDDNSAVYRALYTVDKAYRNNSLTSIKSVVRPFAYLISTD
                     QDPDAVAERLAYTGAVIERLTEATTLPAEIYTIDATGTDAGDRGSDYFPMRGLSHVIT
                     AVSTSTANRTFPKGTYVVYMDQASATHAALALEPMGNRNYGNYWLSRRAENLNGWGGF
                     IPAELGREYPVYRYMTAKKLATAPVYLQRPFVEGARVQRCLPLDRTQRGEIEKKIGKR
                     VAFGFYFSVEGATDPFTLIVPKKYRTTSLGSWYVYNWATEKFVSLKYDGTTVRTLPIS
                     NAYVSSTGEVLAVALQGDSEEDSGGCAVGGVASGMGSILLVTPLLLLWVRKRF"
     gene            complement(3604..4719)
                     /locus_tag="PL162_06570"
     CDS             complement(3604..4719)
                     /locus_tag="PL162_06570"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HQF91718.1"
                     /translation="MSAGGGTKLPERLGTIELEEISLESGRGLRRAPQAYAIYGDRDT
                     AKDNAVLVCHALTGSHRLAGEDLPGQPRPWWTPLVGPGKALDTAKLCVICVNVLGSPY
                     GSASPLSPDPATGDPYRMSFPLVTVRDMVRAQRATLERLGVRHLRAVVGGSLGAMQAL
                     EWLATCPDFVDGGVFLAGPLALYPQAIAFNEVQRRAIQADPAWMEGNYPPGEGPHKGL
                     AVARMMATITYRSEQSFVGRWKREMAQGDPLDWGGTFQVESYLHHQGEELVRRFDANC
                     YLYLTRAMDLHDVQRQRDDDDLRRAFEGKPVMGVGVSSDLLFPNWQVQEAVRSLTRWG
                     AEASYDEIESDHGHDAFLIHADQVDEMIRRFFIQTLS"
     gene            complement(4716..6008)
                     /locus_tag="PL162_06575"
     CDS             complement(4716..6008)
                     /locus_tag="PL162_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HQF91719.1"
                     /translation="MEDPSHRFATRALHAGWHHDPATGAFGVPIYPTAAFQFDSSDHA
                     ASLFSLETPGHIYSRLSNPSVAIFEERMSSLEGGVASVATASGQAAATLLVATLCNAG
                     DNVVAGRSLYGGTLTLLKNLFARFGVTPRMVNTDHPAEVKEAMDENTRAIFVETLGNP
                     GLNVAPLEELGAMADGARVPLVVDNTFASPALCRPLEWGASVVFHSATKYLSGQGSVL
                     GGVVVDGGNFDWTGGRWPQFTDPDPAYHGVVFAEAFGRGALGARLRCHTLRDMGGCLS
                     PFNAYLLTLGLTTLELRMGRHSENALILAEFLEAHPAVAWVRYPGLPSHPHFDLARRY
                     LPSGGGGMMAFSLRGGREAGKRFVEHLKLFGHVANVGDVRSLVVHPASTTHAQLSEAE
                     RSAAGVEEGLIRLSIGLEDPEDLRADLAQALRAAGRDA"
     gene            complement(6083..7099)
                     /locus_tag="PL162_06580"
     CDS             complement(6083..7099)
                     /locus_tag="PL162_06580"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HQF91720.1"
                     /translation="MRVAVFGATGMVGREMLSVLEERRFPAEEVIALASPRSEGTPLP
                     FRGGELRVRAVEERHFAGVDLAIFSAGGGASREWASVAVRQGAVVVDNSSAWRMDPAV
                     PLVVPEVNPGDALRHRGIIANPNCSTIITLMALAPLHREAELEYFSAVTFQSVAGSGR
                     KGVEELERTARDALGGADPQGSLYPHPIAFNVLPQVGNFDDGGVSDEEWKMVRESRKI
                     LGLPGLDVSSTTTRVPVFCGHSVAVTARFRRDLSPERAREVLAQAPGVLVEDDPSRGF
                     YPHPRRAAGTDPVYVGRLRRDTGMGNALAFWVVGDNVRKGAALNAVQIGELLLRENSL
                     GKRS"
     regulatory      complement(7223..7327)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(7499..8359)
                     /locus_tag="PL162_06585"
     CDS             complement(7499..8359)
                     /locus_tag="PL162_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQF91721.1"
                     /translation="MIVDAHVHIYPPSVVAEWERIAAREPYFDALARSSVHRWAQAED
                     LVARMDADGVDCSWVCGFGFQDLGLCRECNDYVEDAARRYPGRIVPLAVVPPLHRGME
                     EEVLRRREAGFLGVGEIFPSGQGWSLTDLRQTWPLAGACFETGMALLIHTAEPVGHAY
                     PGKGSDGPREAATFCEHHPEVTVIFAHWGGGLWLYELMPEMKIILENAWYDTAATPFL
                     YTPEAASAAWAAGVGKKILYGSDFPLLGRDRYDRMWDKIPWGPEDRRDLMGKNAETLR
                     RRLLDFRNGL"
     gene            complement(8356..9267)
                     /gene="metF"
                     /locus_tag="PL162_06590"
     CDS             complement(8356..9267)
                     /gene="metF"
                     /locus_tag="PL162_06590"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HQF91722.1"
                     /translation="MARHIKDLFAAKRPVISFEVFPPKPGVRVDGLLDTLGALRELNP
                     DYVSVTYGAGGSSRARTVEIASAAQNRWGLTALAHLTCIGQTRRDLEEVLEELEQEGI
                     TNVLALRGDPRDDCPPLDPPPFRYAADLVAYLRNSRKGDFCVGAAAYPEGHVECDDLE
                     ADLRHLKEKVDAGVDFLVTQLFFDNEIFFRFMDRLRKLEIGVPVSVGIMPVLNSRQIG
                     RMVSLCGASVPPKFARILARYENAPEALRQAGIAYATEQIIDLLSWGVAGIHIYTMNK
                     PEIAQDIMKNIGVVREALEQGPKETPL"
     gene            complement(9274..11670)
                     /locus_tag="PL162_06595"
     CDS             complement(9274..11670)
                     /locus_tag="PL162_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HQF91723.1"
                     /translation="MNGKALKEWIRQYGRVPLMDGGMGSLLAERGWSPPALPEEMNLN
                     APDVVASVHRDYVAAGAVMVETNTFGGSPLKLAHRDLEGQAREINARGARIARDAVGP
                     RVLVAGSVGPLGDLLEPFGPLSFDRARGAFREQIIGLVEGGVDFILIETMMDLREAKA
                     AVLALKDVAPDTAFVVSFTFSEKGHTVTGTPPAVAAAWARLVGAAGVGANCGVGPDAY
                     LPVVEELHAFGGIPIFVYANAGLPGDEDFWDPFRFARSARALALAGATVIGGCCGTTP
                     AHIKAMGDALRPVATMPLRELHAAPLASRRRVHLAGTSHPLLVVGERLNVSRKSPLKE
                     DVAAFRWDRVREEARRQTAAGAFALDINVGLPQIDQARAMREAVRAAEAASDLPLSLD
                     SDAPDVLEAGLLEATGVPLINSTTAKRKNLDAALETAKRHGACLVVLAIDDDGMADSV
                     DRRVAIAHHVAEAADAHDFPRWAILFDALCLAVGADPKGPQGTLDALRRIKPMGFATL
                     LGISNVSHGLPARALLNRTYLAMALGAGLDAVLADPCDEDTMAMVSAANVLTGRDPQA
                     RRYIEKFSSVPSPEASGASPESREELPPLARLEEAILLGEVEKALAAARELASLSPME
                     LVNQGVVPALDKVGRRYDSGEYFLPQLIASAQAAQAVCDDAMSRLGEQGESLREQVIL
                     ATVEGDLHDLGKNVVGTVLRSHGYRVRDLGKNVPLEELLRAAREENVALVGLSALMTT
                     TMREMERAVRVFREELPGTKVIVGGASVNQEFADRIGAAGYARDALGAVKLVDRLLKE
                     "
     gene            complement(11890..12264)
                     /gene="rplL"
                     /locus_tag="PL162_06600"
     CDS             complement(11890..12264)
                     /gene="rplL"
                     /locus_tag="PL162_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HQF91724.1"
                     /translation="MNRDELIKAIEEMSVLELSELVKALEEKFGVSASAPAMAMPMMM
                     AAAPAAVEEEKTEFDVILKSAGATKINVIKVVRELTGLGLKEAKDLVDGAPKPVKEGA
                     QKAEAEEIKKKLEEAGAEVELK"
     gene            complement(12335..12868)
                     /gene="rplJ"
                     /locus_tag="PL162_06605"
     CDS             complement(12335..12868)
                     /gene="rplJ"
                     /locus_tag="PL162_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HQF91725.1"
                     /translation="MPTAARMQVVAECREALARSSAVFMVEYRGMTVKKMTAIRVLVR
                     RAGGEMKVAKNTLFSIAMQEEGLTPPENIMTGANAYTLVYGDVAAVAKTLRDFSKEKG
                     NEALIIKGGLLGNKLLSANDVFALADLPSREVLLAQVLGTMMGPVRGLVTVLSGTVRG
                     LVTCLDQIREQKEKQAA"
     misc_feature    complement(12882..13047)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(13152..13859)
                     /gene="rplA"
                     /locus_tag="PL162_06610"
     CDS             complement(13152..13859)
                     /gene="rplA"
                     /locus_tag="PL162_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HQF91726.1"
                     /translation="MSKLSKRYKALTEKVDRQKLYGLREAVDLGKSLATAKFDESVEL
                     HVRLGVDPRHADQQVRSTVVLPHGTGIVKRVLVIAAGEKMAEAEAAGADHVAGEDVVS
                     RIQGGWLEFDAVIATPDVMKVVGRLGKILGPRGLMPTAKTNTVTFDVADAVKEIKAGR
                     VEFRVDKTAIVHNSVGKASFDNQKLFENVATLMRAILKAKPAAAKGQYVRSVTLSTTM
                     GVGIKIDPNAAQKECVE"
     gene            complement(13945..14370)
                     /gene="rplK"
                     /locus_tag="PL162_06615"
     CDS             complement(13945..14370)
                     /gene="rplK"
                     /locus_tag="PL162_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HQF91727.1"
                     /translation="MAKKVVGEIKLQLPAGKATPAPPVGPALGQHGVNIMEFVKQFNA
                     KTADQPGMVIPVVITVYADRSFTFIMKTPPASVLIRKTLGLDKGSNEPNKKKVGILTQ
                     KQVEDIARLKMPDLNANTVEAAMEMVKGTARSMGVEVRA"
     gene            complement(14431..14976)
                     /gene="nusG"
                     /locus_tag="PL162_06620"
     CDS             complement(14431..14976)
                     /gene="nusG"
                     /locus_tag="PL162_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HQF91728.1"
                     /translation="MMNENIERRWYIIQTYSGYENKVKANLEQRIASMGMGHRIFSVL
                     VPVEERLQVKEGKAKRTRRKVFPSYVLVEMVLDDQSWFVVRHTPGVTGFVGAGNHPIP
                     LSEREVQDIMTKLGKEQAKPRVEIDLRPGDTVRVRSGPFEGQAGPVVEVMADKGKIKF
                     SVTVFGRETVVEADYSELQKV"
     gene            complement(15032..15214)
                     /gene="secE"
                     /locus_tag="PL162_06625"
     CDS             complement(15032..15214)
                     /gene="secE"
                     /locus_tag="PL162_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HQF91729.1"
                     /translation="MEKLMEFLREARAELKRVTWPGKQQVWYSTLIVIAFTFAVSAYL
                     GIVDVLLTAVFSRVVG"
     gene            complement(15244..15319)
                     /locus_tag="PL162_06630"
     tRNA            complement(15244..15319)
                     /locus_tag="PL162_06630"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15284..15286),aa:Trp,seq:cca)
     gene            complement(15340..15489)
                     /gene="rpmG"
                     /locus_tag="PL162_06635"
     CDS             complement(15340..15489)
                     /gene="rpmG"
                     /locus_tag="PL162_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HQF91730.1"
                     /translation="MADIVSLQCTECKRKNYGTTVNKKKQQKKLEKKKFCKWCGGHTL
                     HKESK"
BASE COUNT         2816 a         5372 c         4558 g         2814 t
ORIGIN      
        1 ggggctcttc ttcggggcct acggcctggc aaaagtcacc ggccactggc aaaccacggt
       61 gagccccgcc gccaacgccg cccgaggggg caaccccgcc gaaggagtct acggttggat
      121 gactttggcc caggtggctg gcgtagtgaa gctccccccc gagaaggtcc tagccatcgc
      181 ggggctcccg gccgacaccc cccaggacgt gtccatgaaa aagatccccg gattgaatga
      241 cgaggccgtc caggaagccc tgagggcaca cttcgccgcc tcgccttccg gaaaggccca
      301 actcccatcc aatccagacg agatccgggg cagcgtcacc ctggaggaaa tcgcccaaac
      361 cttcgacctg gatcccggga aaatactgga ggccgccgga tggcccgccg attccccccg
      421 agatcgtccc ataaaagaag tcggggcggc gctgggcaag gaagtgtcgg agatccgtgc
      481 ggcggtggcc gcgctcaaaa agggcgccaa gtagctcccc gtagcgaaac gacaaaaata
      541 ccgcgagccc caaggctcgc ggtacaattt tttggcagcc cctaggggat tcgaacccct
      601 gtcaacgggc tgagaacccg gcatcctgga ccactagacg aaggggccgc cttatgaaga
      661 aaaagatggc tggggaacgt ggactcgaac cacgactacc tgatccagag tcaggcgtcc
      721 tgccgattag acgattcccc accgctcttt cgccgccgtc tcaccggctg caggaggtat
      781 cataccagca tatccttcgt agcgcaaggg ggtaaaacaa aaccggccga ggggcttgtt
      841 ttaccactcc acaccggtta ttctttcctt tagaggggca ggagcggagc tccttcccct
      901 gtcccgccaa agacccgaag atcgggaggt gatccccttc tagcccacct agaagccccg
      961 tgcgccccgc cgcatcgtcc cctcctcccc cgcaccctag ggaggaaaaa tttggaaaga
     1021 tcaaggagga atcatcgtga ccttcttctc gtaccgtcga tgccttgccc tcgccctcgt
     1081 gggggccacc gccctcgtgg ggagctctgc ggcgtttgcc cgaaaccaag accgatacct
     1141 ggagcggcac cccgacgccc ccagggctct tcgcctcgcc gccccccagg ccattcctgc
     1201 ggtgccctat ggcgggaaca acgaaagcct ttcagactac cccgacctcg tgcagcagtg
     1261 gtttgacgcc aagaccgagg gagtcacgtt ctttaccccg gctttcacct cgaatcacgg
     1321 tagctttgag cagggcggcg tcagctgggg caccacccag gaagaaatga tctctttcct
     1381 gaacgccctt cccaaggact acctcaccat gcgcatcatc ggagaagtgg ccacccggga
     1441 caacaagggc gtggtggagg gatccttcaa agtgcccctc ctggtcttca gcaaacccgc
     1501 cgcctttgac cccgccgacc tccacgccaa gaaaaagccc atcgtctaca tccaggccat
     1561 gatccacggc aatgagacct cgggatgtga agcggtcctc cgcctcgctt cccagctcgc
     1621 ccgggacgac cttaaggtgc tggacaaaat ctccgtagtt ctgctgcccc ggtacaacgt
     1681 ggacggcgcc tggaaatacc agcggggcac ggacaccatc agccccgtgc gggtcaacat
     1741 ggaccagaac cgggaccaca ccgccttcga atcgcccctc acccggctca tgcacaccgt
     1801 ggccaatcaa taccaacccc acgtggccct ggacctgcac gagatgaact acgaccacac
     1861 ggcggactac cgggtctccg gcggagattt tgtccgcttc cgggactacc actactttga
     1921 cctcaccacc ctggtggcac acctctacaa cgtccctgcg ggcatcacca ccctggcccg
     1981 ggagatggag gcccagatca gcgcggacct aaccgcccgg ggtttggcgt ggaacttcta
     2041 cttctatccc tgggacatga cccaggaacg ggccgagatc cagatcggag gcctgccggt
     2101 ggcggtgtcc cgggatgtga tcacggagat catggagggc ccccccgacg aggccatggg
     2161 ggactccgtc ctctccctta ttccctccat cggcatgctc tttgaaaccc gatccccccg
     2221 ggtgctggtg aactacaagc cccgggtcta cgcccacgcc acggccctgg agtccgtgat
     2281 taaccaaacc gcatcccggg ccgacaccat ccgcaacacg gtggagtcgg ggcgggccgc
     2341 catggccaac ctgggcaagt cggtgagccc caccaacacc atcaccctct ggatacgcca
     2401 ggggcaggtc tcccacctct ccttccccgt gttgcgcatg agcgacgaca acagcgccgt
     2461 ataccgggcg ctctacacgg tggataaggc ctatcgcaac aactctctca cctccatcaa
     2521 gagcgtggtg cgcccctttg cctatctcat ctccacggac caggaccccg acgccgtggc
     2581 ggaacgcctc gcctacacgg gggccgtcat cgagcgcctc acggaagcca ccaccctccc
     2641 cgcagagatc tacaccatcg acgccaccgg caccgacgcg ggagatcggg gtagcgacta
     2701 cttccccatg aggggtcttt cccacgtcat caccgccgtg agcacctcca cggcgaaccg
     2761 caccttcccc aagggaacct acgtggtcta catggaccag gcctccgcca cccacgccgc
     2821 cctggcactg gagcccatgg gcaaccggaa ctacggcaac tactggctaa gccgccgggc
     2881 ggaaaacctg aacggttggg gcggcttcat cccggccgaa ttggggagag agtacccggt
     2941 ctaccgctac atgaccgcca agaaactcgc cacggccccc gtttacctcc agcgcccctt
     3001 tgtggaggga gcccgggtgc agcgctgcct ccccctcgac cggacccagc gtggggagat
     3061 cgaaaaaaag atcggcaagc gcgtcgcctt tggcttttat ttctccgtgg agggagccac
     3121 ggaccccttc accttgatag tccccaagaa ataccgcacc acatcgctgg ggagctggta
     3181 cgtgtacaac tgggccaccg agaagtttgt gtccctgaag tacgatggca ccacggtgcg
     3241 caccctcccg atttccaacg cctacgtgtc ctcgacggga gaggtcctcg cggtggccct
     3301 ccagggagat tccgaagaag acagcggcgg ctgcgccgtg ggaggggtcg cctccggcat
     3361 gggatccatc ctcctggtga ctcccctgct cctcctgtgg gtccggaagc gcttctgatc
     3421 ccctcgccgt ttcccaaaag acccacgtag aaagacgcca gggcccgggg gtggcattcc
     3481 ccgggccctt gatgttttaa gtcctcaccc tctggtgcca cggaaacctt ctcaggccct
     3541 ccgctcatac ccaatccctc aaaaaaccgg ctggagagcg catggccggg gcaaagggaa
     3601 ggttcaggag agggtttgga tgaaaaacct tcgtatcatc tcatccactt gatccgcgtg
     3661 gatgagaaag gcatcgtggc catggtcgct ctcgatctcg tcgtaggagg cttcggcgcc
     3721 ccatcgggtc agggatcgca ccgcctcttg cacctgccaa ttgggaaata ggagatcgct
     3781 ggacaccccc actcccatga caggtttccc ctcgaaggcc cgtcgcaggt cgtcgtcatc
     3841 cctttggcgc tggacatcgt gaaggtccat ggctctagtc aaataaagat agcaattagc
     3901 atcgaagcgc cgcactagct cttctccctg atgatgcaaa tagctttcca cctggaaggt
     3961 gcccccccag tcgagggggt ctccctgggc catctcgcgc ttccaccgtc ccacgaagga
     4021 ctgctcgctg cggtaggtga tggtggccat catgcgggcc acggcgagac ccttgtgggg
     4081 cccctccccc ggagggtaat tcccctccat ccacgccggg tcggcctgaa tggcccggcg
     4141 ctggacctcg ttgaaggcga tggcctgggg gtagagggcg aggggccccg ccagaaagac
     4201 ccccccgtcc acgaaatccg ggcaagtggc caaccactcc agggcctgca tggccccgag
     4261 agatcccccc accacggcgc ggaggtgacg cacccccagg cgttccaggg tggcccgctg
     4321 ggcccgcacc atgtcccgca cggtgaccag agggaaggac atgcggtagg gatcccccgt
     4381 ggcgggatcg ggggaaaggg ggctggcgct cccgtaggga cttcccagga cgttcacgca
     4441 gatcacacag agtttggccg tgtccagggc ctttcccggc cccaccagag gggtccacca
     4501 ggggcgaggt tgccccggaa gatcttcccc cgccagacga tggcttccgg tgagggcgtg
     4561 gcacaccagc accgcgttat ctttggcggt gtcccgatct ccgtagatgg cataggcctg
     4621 gggggcccgc cgtagccccc gcccggactc cagagagatc tcttccagtt cgatggttcc
     4681 cagacgttcg gggagcttcg tcccccctcc ggcgctcatg cgtctcgacc cgccgcccgg
     4741 agcgcctggg ccagatcggc ccggaggtct tcggggtctt ccaatccgat gctcaggcga
     4801 atgagcccct cctccacccc cgccgccgac cgttcggcct cggagagctg ggcgtgggtc
     4861 gtgctggccg ggtgcaccac caggctccgc acgtccccca cgttggccac atgaccaaag
     4921 agcttcaaat gttccacgaa gcgcttcccc gcctccctcc ccccccggag gctgaaggcc
     4981 atcatgcccc cccctccgga gggcaggtag cgccgggcga ggtcgaagtg ggggtgagag
     5041 ggcagtcccg ggtaccgcac ccaagccacc gccgggtggg cctccagaaa ttccgccaga
     5101 atcagggcgt tttcgctgtg gcgccccatg cggagctcca gggtggtgag ccccagggtg
     5161 aggaggtagg cattgaaggg ggataggcat ccccccatat ctcggagggt gtggcaccga
     5221 agacgggctc ccaaggctcc ccggccgaag gcctcggcaa agaccacgcc gtggtaggcc
     5281 ggatcagggt ccgtgaactg gggccaacgc cccccggtcc agtcgaagtt ccctccgtcc
     5341 accaccaccc ccccgaggac gctcccctga cccgaaaggt acttggtggc ggaatggaac
     5401 acgacggagg ccccccactc cagggggcga cacaaggcgg gagaagcaaa ggtgttgtcc
     5461 accaccagag gcacccgcgc cccatcggcc atcgccccca gctcttccag gggcgccacg
     5521 ttcagcccgg ggttccccaa ggtttccacg aagatcgccc gggtgttctc gtccatcgcc
     5581 tctttgacct cggcggggtg atcggtgttc accatgcggg gggtcacgcc gaagcgggcg
     5641 aagaggtttt ttagcagggt cagggtaccc ccgtaaaggc tccttcctgc caccacgttg
     5701 tctccggcgt tgcagagcgt cgccaccagg agcgtggccg ccgcctgccc cgaggcggtg
     5761 gccacgctgg ccacgccgcc ctccagggag ctcatgcgct cttcgaagat ggccaccgag
     5821 gggttggaaa ggcggctgta gatgtgcccc ggagtttcga gggaaaaaag actggcggcg
     5881 tgatcggagc tatcgaactg gaacgccgcc gtggggtaga tgggcacgcc aaaggccccc
     5941 gtggcggggt cgtggtgcca tccggcgtgc agggcccggg tggcgaaacg gtgggacggg
     6001 tcttccatgg cctggagcct cctttatgtc ttttattcct gatgcctgga gggccccctc
     6061 tcctcgggaa ggcccaggtc cctcaggatc tcttccccag ggagttttcc cgcaggagca
     6121 gctcgccgat ctgcaccgcg ttgagggcgg cccccttgcg cacgttgtcc cccaccaccc
     6181 aaaaggccag ggcgttaccc atgcccgtgt cacggcgaag cctccccacg tagaccggat
     6241 cggtgcccgc cgccctccgg gggtgggggt agaacccccg ggaggggtcg tcttccacca
     6301 acactccggg ggcctgagcc agtacctccc gggctctttc gggggaaaga tctcggcgga
     6361 atcgggcggt caccgccacg gagtggccgc aaaagaccgg cacccgcgtg gtggtggagc
     6421 tcacgtcaag ccccgggagt cccaggatct tacgggattc ccgcaccatc ttccactcct
     6481 cgtcgctcac ccctccgtcg tcgaagttcc ccacttgggg aaggacgtta aaggctatgg
     6541 gatggggata gaggcttccc tgggggtcgg ccccccctaa ggcgtcccgg gcggtgcgct
     6601 ccaactcctc tactcccttg cgccctgacc ccgccacgga ctgaaaggtc accgcgctga
     6661 aatactctag ctccgcctcc cggtggaggg gggccagggc catcaaagtt atgatggtgg
     6721 agcaattggg gttggcgatg atgccccgat ggcggagggc gtcgccgggg tttacttcgg
     6781 ggaccaccag gggcacggcg gggtccatgc gccaggccga gctgttatcc accaccacgg
     6841 ctccctggcg caccgccacg gaggcccact cccgggaagc cccgcctccg gcggaaaaga
     6901 tcgccaaatc gactccggcg aagtgccgtt cttccacggc cctcacccga agctctcctc
     6961 cccggaaggg caaaggcgtt ccctcggagc gaggggaagc cagggctatc acttcttcgg
     7021 cgggaaagcg ccgctcttcc aatacagaaa gcatttctcg ccccaccatg ccggtggctc
     7081 caaaaaccgc aacgcgcacg atcccgaccc ctctcccatc aaacccctcc caagggggcg
     7141 ccccgcgatc ctccgggcgt ccctccttgg cccgatgaac aaaaaagctc cccatggaga
     7201 agggagcttc gtgaacccat ttctctcatc atttccgccc cgaaggcggc aggtcttagc
     7261 accatgcccc tcgggggccg gttgccgggc gtcatcgggc ctgtccctcc gccgctcgcg
     7321 ataagaggtt ctattggtgc gaagcccccg ggcttcgctt gtgttggggg agattatagt
     7381 cacaaagggc aagggctgtc aacggcccca tcggctgagg gtgccccctt cgccgggcca
     7441 tccgggcgaa gtacttacgg aaaactctcc cccttagatc cccggggggc gccgtctttc
     7501 acaggccgtt acgaaaatct agaaggcgtc gccgcagggt ctccgcattt ttccccatga
     7561 ggtccctccg gtcttcgggg ccccagggga tcttatccca catccggtcg tagcgatccc
     7621 gccccaggag gggaaaatcg ctgccgtaga ggattttttt ccccactccg gcggcccagg
     7681 cggcggacgc cgcctcaggg gtgtagagaa agggagtggc ggcggtatcg taccaagcgt
     7741 tttccaagat aatcttcatc tcgggcatga gctcgtagag ccatagccct ccgccccagt
     7801 gggcgaagat cacggtcacc tccggatgat gctcgcaaaa cgtcgctgct tcccgggggc
     7861 catcggagcc cttgccgggg taggcgtgtc ccacgggttc cgccgtgtgt atcaaaaggg
     7921 ccatccccgt ttcaaagcac gcccccgcca gaggccaggt ctggcggaga tccgtcaggc
     7981 tccacccctg ccccgagggg aagatctctc ccactcccag aaaccccgcc tcgcggcggc
     8041 gcaatacctc ttcttccatc ccccggtgca gggggggcac caccgccaga ggaacgatgc
     8101 gtcccggata gcgccgcgcc gcgtcctcca cgtagtcgtt gcactcccgg cagagcccca
     8161 aatcctgaaa gccaaaaccg cagacccagg aacaatccac tccatcggca tccatgcgag
     8221 ccaccagatc ctcggcctgg gcccaccgat gcacggagct ccgggccagg gcatcgaagt
     8281 agggctcccg ggcggcgatg cgctcccatt ccgccaccac ggacggggga tagatgtgca
     8341 catgggcgtc gacgatcaca gtggggtctc cttcggccct tgctccaggg cttcccgcac
     8401 cacgccgatg tttttcatga tatcctgggc gatctcgggt ttattcatgg tgtagatatg
     8461 aattcccgcc accccccagg agagaaggtc gatgatctgt tctgtggcgt aggcgatccc
     8521 ggcctggcgc agggcttcgg gggcgttctc gtaccgggcg aggatgcgtg cgaacttggg
     8581 aggcaccgag gccccgcaaa gcgataccat gcgcccgatc tgccgggaat tcagcacggg
     8641 catgatcccc acggacacgg gcaccccgat ctccaacttc cgcaggcgat ccatgaaacg
     8701 gaagaaaatc tcattgtcga aaaaaagctg ggtgacgaga aaatccaccc cggcatccac
     8761 cttttctttc aggtggcgta gatccgcctc taggtcgtca cactccacgt ggccttcggg
     8821 gtaggctgcg gcccccacgc agaaatctcc cttccgagaa tttctgaggt aggccaccag
     8881 gtccgccgca tagcgaaagg ggggaggatc cagcggggga cagtcgtccc gggggtctcc
     8941 ccggagcgcc aggacgttgg tgatcccctc ttgctccagc tcttcgagca cttcttccag
     9001 gtcccgtcgg gtctggccaa tgcaggtaag atgggccagg gcggtgagtc cccaacggtt
     9061 ttgggccgcc gaggcgatct ccaccgtgcg cgcccggcta cttccccccg cgccataggt
     9121 gacgctcacg tagtccggat tgagctcccg caaggccccc aaggtatcca gtaggccgtc
     9181 cacccgcacc cctggtttgg ggggaaagac ctcgaaggaa atcaccggcc gcttcgccgc
     9241 aaaaaggtct ttgatatggc gcgccatgac gccctactcc ttgagaagac ggtccacgag
     9301 cttcacggcc cccagggcat cccgggcgta gcccgcagca ccgatgcggt ccgcgaattc
     9361 ttggttcacc gaggctcccc ccacgatgac cttcgtccca gggagctcct cccgaaagac
     9421 ccgtaccgcc cgctccatct cccgcatggt ggtggtcatg agggccgaaa gacccaccag
     9481 ggccacgttt tcttcccggg ccgcccgcag cagctcttcc agggggacgt ttttccccag
     9541 gtctcgcacc cggtagccgt ggctccgcag caccgtaccc accacgttct tgcccaggtc
     9601 gtgcaggtct ccctccaccg tggccagaat gacctgttcc cggaggctct cgccttgctc
     9661 tcccagacgg gacatggcgt cgtcgcacac ggcctgggcg gcctgggccg aggcgatgag
     9721 ctggggcagg aaatactccc cactgtcgta gcgtcgcccc accttgtcca gggctggtac
     9781 caccccctgg tttaccaatt ccatggggga cagggacgcc agttcccggg ctgcggccag
     9841 ggctttctcc acctccccca ggaggatggc ctcttccagg cgtgccaagg gcggaagctc
     9901 ttcccgggac tccggactcg ctccggaggc ctccggggaa ggaaccgacg agaacttctc
     9961 gatgtaacgc cgcgcctggg ggtcccttcc cgtgaggacg ttggccgcgc ttaccatggc
    10021 catggtatct tcgtcgcagg ggtccgcgag gaccgcatcc agccccgccc ccaacgccat
    10081 ggctagatag gtacgattca acagggctcg ggcgggtagg ccgtgggata cgttgcttat
    10141 gcccagcaaa gtggcaaagc ccatgggttt gatgcggcgc agggcgtcca gggtcccctg
    10201 gggccccttg ggatccgctc ccacggcaag gcacagggcg tcaaaaagga tggcccaccg
    10261 gggaaagtcg tgggcgtcgg cggcttcggc cacgtgatgg gcaatggcca cccgccggtc
    10321 cacgctgtct gccatgccgt cgtcgtcgat ggccagcacc acgagacaag ctccatggcg
    10381 cttggccgtc tccagggccg cgtccaggtt ctttcgcttt gccgtggtgg aattgatcaa
    10441 gggaactcct gtggcctcca agagccccgc ctccagcacg tcgggggcgt cgctgtccag
    10501 ggataggggg aggtcggagg cggcctcggc ggcccgcacc gcctctcgca tggccctagc
    10561 ctgatcgatt tgggggagtc ccacgttgat gtccagggca aaggccccgg cggcggtctg
    10621 gcgacgggcc tcctcccgga cgcgatccca gcggaaggcc gccacgtcct ctttcaaggg
    10681 agacttgcgg gagacgttca ggcgctctcc caccacaagg agtggatggg aggtccccgc
    10741 cagatgtacc cggcggcggc tcgccagggg ggccgcgtga agctctcgca ggggcatggt
    10801 ggccacgggg cgaagggcgt ctcccatcgc cttgatatgg gcgggggtgg taccgcagca
    10861 tcctcctatg accgtcgccc ccgccagagc cagggcccgg gcgctccggg caaagcggaa
    10921 gggatcccag aaatcctcgt ctccgggaag acccgcgttg gcgtagacga aaatgggaat
    10981 tcctccgaag gcgtgaagct cctccactac gggaagatag gcgtcgggcc ccacaccgca
    11041 gttagctccc acccccgccg cccccaccag ccgggcccag gccgccgcca cggcaggggg
    11101 agtgccggtg accgtgtgcc ccttttcgct gaaggtgaag ctcaccacga aggcggtgtc
    11161 gggagccacg tccttgaggg ccaggactgc ggccttggct tctcgcagat ccatcatggt
    11221 ctcgatgaga ataaagtcca cccccccctc caccaacccg atgatctgct cccgaaaggc
    11281 ccctcgggcc cggtcgaagg acagggggcc aaagggctcc aatagatctc ccagaggccc
    11341 cacagagccc gccaccagca ctcggggccc caccgcatct cgggcgatgc gagccccccg
    11401 ggcgttgatc tctcgggctt gcccctccag gtcacgatgg gcgagcttca gaggacttcc
    11461 tccgaaggta ttcgtttcca ccatgacggc cccggcggcg acgtagtccc gatggacgct
    11521 ggccaccacg tcgggggcgt tcaggttcat ctcctccggc aacgccgggg gggaccaccc
    11581 ccgctccgcc agaagcgatc ccatgccgcc atccatgagg ggtacccgac catactgacg
    11641 aatccactcc ttgagggctt ttccgttcac gccgcacctc catcgtctat ctgtcgcccg
    11701 atcgtggcgt ctttttcgcc acccatccgc tccataccgt catcatagga cgcctccctc
    11761 tcctaggcaa gctccgcgat tctattgata tttaccgccc ggggcttccg gagcctctcc
    11821 ccgaacggat ataaaaaggg acggcgggtt tttccgccgt cccgcttctg aactatgtcc
    11881 ctgaggcgat tacttcagct cgacctcggc tccggcctct tcgagcttct tcttgatttc
    11941 ctcggcctcg gccttctggg ccccttcctt cacaggcttg ggagctccgt ccaccaggtc
    12001 cttggcttcc ttcaggccca ggccggtgag ctcgcgaacc actttgatga cgttgatctt
    12061 ggtggctccg gcgctcttca ggatcacgtc gaattcggtc ttctcctctt cgacggcggc
    12121 gggggcggcg gccatcatca tgggcatggc catggcgggg gcggaggcgg agacgccaaa
    12181 cttctcctcc agagccttga ccagctcgga aagctccagc acggacatct cttcaatcgc
    12241 cttgatcagt tcatcgcggt tcatgggggt cacactcctt ggaatttttt acggaccctc
    12301 tggggcccgg atctttctgt aatccttacg gcccttaggc cgcctgcttt tccttctgct
    12361 ctctgatctg gtcgagacac gtgaccaaac cccggacggt gcccgacagg acggtgacca
    12421 gtcctcggac ggggcccatc atggtcccca gcacctgggc caggagcact tcgcggctgg
    12481 gaagatccgc cagggcaaag acatcgttcg ccgaaaggag cttgttcccc aggaggcccc
    12541 ccttgataat cagggcctcg ttaccctttt cctttgaaaa atccctaagg gtcttggcca
    12601 cggcggccac gtcaccgtac accagcgtgt aggcgttcgc tcccgtcatg atgttttccg
    12661 ggggagtcag cccctcttcc tgcatggcaa tggaaaaaag ggtgtttttg gccaccttca
    12721 tttcgccccc ggcccggcgg accaagacgc gaatggccgt catcttcttc acggtcattc
    12781 cccggtactc caccatgaag accgccgaac tccgggcaag ggcctcccgg cattccgcga
    12841 caacctgcat acgtgcagct gttggcattt tagtttcacc tcctcacaca aaaaaatctc
    12901 ccggaaccga ggcgttccgg gagaccatga cactcctctt cagtcaggca agcctggagg
    12961 gcgcacgctc cgggacctcg gcgggctctc gcgatttagg cgcccggagg gcgcacccgc
    13021 tgtctacggt accgaacaag ctatcgactt gtggtcaagg gcgatccgaa aagatcaccg
    13081 ccgcttgtta taacagaaaa atagttttcg cgcaaggggc ccgaaaaacg cggggaaact
    13141 cgcgagaaac gctactccac gcactccttc tgggcggcgt tgggatcgat cttgatgccc
    13201 acgcccatgg tggtgctcaa agtcacgctc ctcacgtact gtcccttggc ggcggcgggc
    13261 ttcgccttga ggatcgcccg catcagggtg gccacgttct cgaagagctt ttggttgtcg
    13321 aagctggcct ttcccacgga gttatggacg atggcggtct tgtcaacccg gaactccacg
    13381 cgccccgcct tgatctcttt cacggcgtcc gccacgtcga aggtcaccgt gttggtcttg
    13441 gcggtgggca tgagtccccg gggaccgagg atcttgccaa ggcgccccac gaccttcatc
    13501 acgtcggggg tggcgatcac cgcgtcaaac tcgagccatc ctccctggat gcgggagacc
    13561 acgtcttccc ccgccacgtg atccgcgccc gcagcttccg cttcggccat cttttcccca
    13621 gcggcgatga ccagcacccg cttgacaatc cccgtgccat ggggcaggac caccgtacta
    13681 cgcacctgct ggtccgcatg ccgggggtct acaccaaggc gcacgtgaag ctccacgctc
    13741 tcgtcaaact tggccgtggc cagggatttc cccaggtcca ccgcctcgcg gagaccataa
    13801 agcttctgcc gatccacctt ctccgtaagg gccttgtagc gcttgctcaa cttggacatg
    13861 tcttcttcct cctttgtggt tcggacggac ctccctacag aaggtcctcc cacaggccgg
    13921 agccaaagct ccgaagcgcc aatcctacgc ccgcacctcc acgcccatgg accgggcggt
    13981 gcctttcacc atctccatgg ccgcctccac cgtgttggcg ttgaggtcgg gcatcttcag
    14041 ccgggcgatg tcttccacct gcttctgcgt aaggataccc acctttttct tgttgggctc
    14101 gttggagccc ttatccagcc ccagggtctt gcgaatgagc acgctcgccg ggggagtctt
    14161 catgatgaac gtgaagctgc gatccgcata gaccgtgata accacgggaa tcaccatgcc
    14221 gggctggtct gcggtcttag cattgaactg cttcacgaac tccatgatgt tcacgccgtg
    14281 ctgccccagg gcgggcccca ccgggggcgc cggggtcgcc tttccggcgg gcagctgaag
    14341 cttgatttca ccgactactt tcttcgccat gtcctttctc ttcctccttg atccgggcgc
    14401 cgtaccttcc ggcccccaag ggtcccactc ctacaccttc tgtagctccg agtaatccgc
    14461 ctccacgacg gtttcccggc caaagacggt gacggaaaac ttgatcttgc ccttgtccgc
    14521 catgacctcc accacgggcc ccgcctgccc ctcaaagggg ccgctacgca cccgcaccgt
    14581 gtcgccgggc ctaagatcga tctccacccg gggcttggcc tgctccttcc ccagcttggt
    14641 catgatgtcc tgcacttcgc gctccgagag gggaatgggg tggttgcccg cccccacaaa
    14701 ccccgtcacc cccggcgtat gccgcacgac gaaccaagat tggtcgtcca gcaccatttc
    14761 cacaagaaca taactgggga agaccttgcg ccgcgtgcgc ttggccttcc cttctttgac
    14821 ctgaaggcgc tcctccacag gcaccagcac gctgaagatc cgatgcccca tgcccatgct
    14881 ggcgatgcgc tgctccaggt tggccttgac cttgttctcg taccccgagt aggtctgaat
    14941 gatgtaccaa cgacgctcga tgttttcgtt catcatttaa aagggggcct tgcggccccc
    15001 ctcctcccgt tgtccctcgt cggcctattg cctagccaac gactcgagaa aacactgcgg
    15061 tgagcagcac atcgacgatg ccgagatacg cagacaccgc aaaggtgaac gcgatgacga
    15121 tgagtgtgga ataccacacc tgctgcttgc ccggccacgt gaccctcttg agctcggccc
    15181 gggcttcacg caggaattcc atcagcttct ccacgtattt cggcctccag tcttacaaaa
    15241 aaatggcagg cccgacagga ctcgaacctg taacccccgg ttttggagac cagtgctctg
    15301 ccagttgagc tacggacctg tgccaaaggg gattataccc tacttggact ccttgtgcaa
    15361 ggtgtgcccc ccacaccact tacagaattt cttcttctcc aacttcttct gctgcttctt
    15421 tttattaacc gtggtgccat aattcttccg cttgcactcc gtgcactgca acgacacgat
    15481 gtctgccatg ctgccaggct cccctctagt cgagaatctg ggtgacgacg cccgctccca
    15541 ccgtgcggcc cccttcgcga
//
LOCUS       DAOOER010000043        15019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_4568_length_15019_cov_7.117348, whole genome shotgun sequence.
ACCESSION   DAOOER010000043 DAOOER010000000
VERSION     DAOOER010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 15019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15019
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4568_length_15019_cov_7.117348"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="PL162_06640"
     CDS             complement(<1..503)
                     /locus_tag="PL162_06640"
                     /inference="COORDINATES: protein motif:HMM:NF025696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HQF91731.1"
                     /translation="MPIPDFQALMLPLLRFASDGQEHSTQEAVETLATEFHLSPAERS
                     ELLPSGQQAIFNNRVGWARSYLKKAGLLESPRRGTLRMTDRGRQVWSERPPTMDVKYL
                     ERFPEFMEFRNGPRKNGPRKNGNDPIAIDPIITPEQTPEEAMEQVHQNLRHSLAQDIL
                     RRVLSCSP"
     gene            complement(751..2634)
                     /locus_tag="PL162_06645"
     CDS             complement(751..2634)
                     /locus_tag="PL162_06645"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQF91732.1"
                     /translation="MENPGGILPKWAREIGRFLSMKPQFVLWGNVHDVYPLPPGGAEV
                     ATPPLPEFLGALLAREGYVLVVRYEPLEGFSLVQGDGALFFSATGEQLSDEGVLPAAL
                     TRAAEIAERIVSCAEFPCALVISCASRLEDLSRHDAPAFFFRMHRLGKRALPRTHRRI
                     SPGALPRPRFNLLFWLLDKENDLPPWYALDNPRVRVLPVPRPDAAIRRELIGALAPSL
                     ASDAEAPVDERKRATAMQLFTDATGGLFTSEIRAIAQGARQEGISLENIGEAIRRYRL
                     GIEENPWSRLNPATMRSAEEFLGERVMGQEYAVRMAANILKRSIFNLSGAQFSRLSQR
                     PKGVLFLAGPTGVGKTELAKAITELVFGAPTHYIRFDMSEFGHEHADQRLIGAPPGYI
                     GYDVGGELTSALKGNPFSVVLFDEIEKAHRKILDMFLQVLDDGRLTSGRGETVYFSES
                     LVIFTSNLGIYDVAPDGTRVARVDPSLPYEELRPRIQDAIGGFFRGIQRPEILNRLGK
                     NIVVFDFLRPATAVRIFHRMLENVLRQLEEDHRITLSFAPEVRQRLGEVVCADLSMGG
                     RGIGNALEEVFVNPLSRGLFEAGIRPGERATVRRIDRGPLGWTLELERAPEGEGEGTG
                     DLV"
     gene            2879..5854
                     /locus_tag="PL162_06650"
     CDS             2879..5854
                     /locus_tag="PL162_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HQF91733.1"
                     /translation="MGGVILSYGRVKDLGADVAAWREGGRQTIFLTPSSEDRELLRGL
                     LAGEKGFFGELPPVWRWNDLYRELGKAVRVAIKGQLDPPDHALVVAHVVRTLIKEADE
                     KGWALPPGVRRGGFKALLGRHLRELLREGVEPEDLTRGLKCPGCSDAPCGNLGHPGGI
                     LCRLFRDYTDWLAAHDLADSAAVPSLAADLLEKELPKKWARERRFVLVGFLSFTSAQL
                     RLVRALGSVAEVVLAKPDPGIEGFYDGAEQLEAWSREKRGDPGGKWHLLEAGTPSLEP
                     EVAVREIALWAAGRSALASRLSWPGFGGVGVVAPREALPLLEEALGRYRVPWSFRGRV
                     SAAETFLGTLPRQIWRAAQGGWGLGETALALAHPLLGGDALRERAGKERPSGEEAWRA
                     FDDRGADKTFKKVADFARMLKEGAPPRTLLEALETLISQGNEGLRRASALADSPLWGP
                     EGPRHGELDGAVRRLAGALEELRRKIRSLEEREKLLEEAGKAFFQGVDALDFLEEWAE
                     EATVAAAPPLGGAVALYADTPPVLATHPVLFFLGLTASRWPGTGADSPLLGDDLRREV
                     NAGERDGAPALHLLLRHERRQQKEALFRRIVAVGEQLTVLSRPQQDSEGRPTAPSPFV
                     ESFTRNSKDAAGENTLVRSAAETLPDAEEPFFPAREALRRGKIAFRGNFPRTASWEPR
                     NRGNFSDLDLFTACPFAYSMARLRKLEEKSWEEEGGGEPLREGTFLHRLWELVWQTPG
                     AGEEGTSLEELVDRYWDQALEPEEGASSGHPYEGYRALREDPRCAPRARFLRFAARRL
                     GRLQDQAEESRRRDGVEVTHRLRESVNPRMVVGGVTFSGRCDRVDLTSQGALLLDYKL
                     GNVAGKKIRRLQLAAYALALEEGRLSGENPLPGGQEIRGFAYLGHRDGKAQGEMEPPF
                     YRWYGMKSSGSLDVAMAEAREALESMAGALQEGKYPANYASENCAWCPYPALCRRGES
                     QGEGMGEDQGDEDEIQEGA"
     gene            5917..>9085
                     /locus_tag="PL162_06655"
     CDS             5917..>9085
                     /locus_tag="PL162_06655"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HQF91734.1"
                     /translation="MIKNAQDVFLRGFRDEQARAITSSEPLVVVSAGAGTGKTQTLAT
                     RFAWLLATDPACRVEQILTLTFTEKAAREMQERIRGRLEEWYRGDPTGMAHLRDALER
                     FDEGYISTIHSFAYRVVRESGLAMDLDPGATLLAPPEEEAFWEEFTAELEGNPSSWVA
                     EGLDDPWGPRAREFFASADFVDLRDGFGASALASAVRETSELAASLGWNPEKLWDWSD
                     DETLETLRKMVDPFARECWELWQCVLIPAVESLLAQDTTRLGTALKNLKERFRGEEAP
                     DRDLMRQFFAAIHDALEKLDGAGKVKKLLEDTLGEGLKSWRDTRKEMMLLARELTADA
                     PEPPEEKRLRQGLRCLAALGWERWDRWRRLRGGLIFRDLIAGATEVLQKDPEAAKRFR
                     HIMVDEFQDTDPLQDALIRALWRPDTNTLFLVGDLKQSIYRFRHADLTLFGTYIDEAK
                     KTPGDSRESYIPLPCSFRLRSSLLDEVNALFGDIWKDGLGKDLPLPYEALTGPEDLPF
                     WKCRNASGGAPVEALLAQTSRKAKGEETSRHGTASEGRARCFSALAARFREIHDRPQG
                     EESLEDRVWDKDGACWRAPRWSDFAVLVPSRGHYPLLEQAFDAAGIPVAFDGSRAYFS
                     RGEVRDVVNLLKALARPEDDLALAGWSASPFSGVLPHQTLEWLSPPGSRQSLTAHAEA
                     PTCPEKIQGPLSGASSRETSSESFPPGSLRDQMARHAPAAARRFDRLRRTARLKGASG
                     ALALLLEDPHALRAFGPSARRRVAANLRAAEALAREYETTLGGELGACAAYLDRALRK
                     EHPREEPTFVGEDEDAVRVLTIHGAKGLEYPFVAVTGLERSPGREKTATITPSRHLGA
                     VFSRIPPLLKGAPAQPPAGSTSAGSAGFQPAHSSPSPDMPAEIASSRGKQRDTGETSA
                     KSEERDTPTTASRWHRLLERQALVEENWRLFYVATTRAREKLFLCGNISMDKDDAAAA
                     APKDSWLGPVRQRLGEGGTAVTCRILSRQDEEILKSPGKNPEMAPSPAPADALPLPSP
                     DPFLGRLGASAYALLRYCPRAYRRSY"
     assembly_gap    9086..9185
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9186..9983
                     /locus_tag="PL162_06660"
     CDS             <9186..9983
                     /locus_tag="PL162_06660"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HQF91735.1"
                     /translation="ASAYALLRYCPRAYRRSYRQGIPLIWDKKDDGPGGSLLGTLAHE
                     VLRGWNFAEEELESWLPEDDEAREQVARRLPPDLRLPLGRAEDRRALASWLRALCRSA
                     EGARLRGRTLRKEVVFRLPLGPRGGGPTVDLVGALDLLWEDDKGLHVRDYKTAPPESI
                     PDDLYRHQLLFYGVVARHCRPGRPVDLALYHLRAFAGEGGDPVTAVTPAPSPEAWEAW
                     DDGEWEAAWCQVEEELWQAARQAAQGPWPPRPEGCHRCPWKSSCAPE"
     gene            10817..11392
                     /locus_tag="PL162_06665"
     CDS             10817..11392
                     /locus_tag="PL162_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR04564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SYNERG-CTERM sorting domain-containing protein"
                     /protein_id="HQF91736.1"
                     /translation="MKKVLLALALLGVFMAGAAFASGHVDPAPFKPIAVDSGDVPLPT
                     GDGGVVSMDLTPAVQGDVPQQGIMELMGGGETAHILSAVKLELVYNNADGEAIIDLTA
                     VIAKVKAATGTEPSVVWITNKKTGKVTEFPISGGKITVKPVGDYFTESVLTFGNKTTA
                     SSGSGGGGGCNAGFAPAMLLLALPLALFIRK"
     gene            11506..13668
                     /locus_tag="PL162_06670"
     CDS             11506..13668
                     /locus_tag="PL162_06670"
                     /inference="COORDINATES: protein motif:HMM:NF017857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HQF91737.1"
                     /translation="MRHGLVLFVGLLGAVLSGSFLSPARALSPGNAGYVGLWEYPTAQ
                     MAEDGHVWVGYADSDPYLTYFVNMAYFPWLEVNLRLTAFSTAPRISEGYGRYKDKALD
                     LKVLLRKGRGFSPSLAVGITDLMGTELLRAKYGVFTWTWDSLAFSLGYGTDRLNGVFG
                     GIEWQAAPSWAFKLEYSPLYYPGDKVSGTRIHSAPAASSWNVGVVYDAPWGGEASLSY
                     QRGEDLCFSVAYAYDLAGPFLGSREKRLAPSTPPPSVSDWDAEDLTLLAEELRRNVGA
                     LGGLRDVRVAVGDHTLLVAFENTGYGSAAEACARVLGAVAWRAPWDLRTLSLLPLLRG
                     QPSVRMDIPGSHCALLRLGDLSREDSAALAVRWATLDDLASEGGAPWRAQAPREGWPA
                     PGTEFRFRFSYEPRIDRILDNDYEDRFNLDAELRHRTSWGWEFYGSVRVPIKNNIDIW
                     WEPETNDDVRIWQTVLSWGTLRALDERRAVALLAEVGWLDANWFGANLWGRAYFDGGR
                     WWLGGRASVVQQRDPHSFGGLSEYWRTYDPSGGWVIGGPKNEWTLGAWAQAGYREPTL
                     DVDLLAEYGRFLDEDWGGRVAVTRHWNDVGVGFWMSRTDILTPGKDFTNAGMYLEIPV
                     DAWFGTRSPHVWTQEFTLLSTWADFAGRQPGAWRTPERLWGQVLPDRLPGQMYALLER
                     FCRAVGKNALPEFSSKAQSLLECFTPGSSWGEDETELY"
     gene            14148..>15019
                     /locus_tag="PL162_06675"
     CDS             14148..>15019
                     /locus_tag="PL162_06675"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HQF91738.1"
                     /translation="MGLPRFGADFFRRTPSVFAPLDTTAGDPGYVVGPGDEVRIDLFG
                     AFNPTGAEDAGGNWTVTVARDGSLALPKAGTVRAAGSTLEGLQQKIRDAYGTYYTDFE
                     LHVTLGRARAFSVYVAGDVKSPGVYAMPARATVALALATAGGPSPAGSLRHIKVMRGG
                     KAVGIFDAYDLLLSGDRKGDLPLQDGDVVFVPPVGILAAITGDVLRPAVYELRSEMPL
                     GSFLQLAGGPTSQQFSGRVQIYRVENAQYRMAFESEADPRALASVQIRHGDVVKFFPI
                     SVALSDVTLAGAVAS"
BASE COUNT         2526 a         4997 c         4848 g         2548 t
ORIGIN      
        1 ggggagcacg aaaggacccg gcggaggatg tcctgggcaa ggctgtgtcg aagattctga
       61 tggacctgct ccatcgcttc ctcgggggtt tgttccgggg tgatgatcgg atctatggcg
      121 atggggtcat ttccgttttt tcgtggacca ttttttcgtg gaccattgcg aaattccata
      181 aattcgggga atcgttcaag gtacttcacg tccatggtcg gaggtctttc gctccacacc
      241 tgtctgcccc ggtcggtcat gcgcaaagtc cctcggcgtg gcgattccaa cagtcccgct
      301 ttcttgaggt aggaacgggc ccaccccaca cggttgttga aaatagcctg ctggccgctg
      361 ggcaagagct cactgcgttc ggcaggagag aggtgaaatt ccgttgccag ggtctcgacg
      421 gcttcctggg tggagtgttc ttggccgtcg gaggcaaatc ggagcagagg aagcatgagc
      481 gcttgaaagt caggaattgg cataagttca cctccggatt gagagtgcct gcttttccat
      541 gggaccgacc cgcagagctt tccaagcggg cgcgtacaaa gcccgtgttg gtcggcgggg
      601 cctcatgtag gcggggcctt gttcgacgta tcctcgccgg cccttcgcac ggtcaccgcg
      661 ctgccggttt cccgcgttgt agggtcggaa aatatgctcg cccctacggc cgatgcgcga
      721 cgtttctcag ggggtgatct ctttgttgcc tcacacgagg tctccggtcc cttccccctc
      781 tccttccgga gcccgttcca gctccagcgt ccatcccaaa ggcccccggt cgatgcgtcg
      841 caccgtggcc cgctctccgg ggcgaatgcc cgcctcgaag agcccccggg acaggggatt
      901 tacgaagacc tcctccaggg cgttgccgat gccccgcccc cccatggaaa gatcggcgca
      961 gaccacctcc cccagtcgtt gccgtacctc cggtgcgaag ctcagcgtga tgcggtggtc
     1021 ttcttccagc tggcgcagca cgttttccag catccggtga aagatccgca ccgccgtggc
     1081 ggggcggagg aagtcgaaga ccacgatgtt tttccccagc cggttgagga tttcggggcg
     1141 ctggatgccc cggaagaacc ccccgatggc gtcctggatg cgggggcgca gttcctcgta
     1201 gggcagggag ggatccaccc gggccacccg ggtgccatcg ggggccacgt cgtagattcc
     1261 cagattgctg gtgaagatca ccagactttc ggagaagtag accgtttccc cccgccccga
     1321 cgtgaggcgc ccgtcgtcca gcacctggag gaacatgtcc aggatcttcc ggtgggcctt
     1381 ttcgatctcg tcgaagagca ccacggaaaa ggggttgccc ttcagggcgc tggtgagctc
     1441 tcctcccacg tcgtagccga tgtaccccgg aggggcgccg atgaggcgct ggtccgcgtg
     1501 ttcgtggccg aactcgctca tgtcaaagcg gatgtagtgc gtgggggccc cgaagaccag
     1561 ctccgtgatg gccttggcga gctcggtttt gcccaccccc gtgggccccg ccagaaagag
     1621 cacccccttg gggcgctggg agaggcggga aaattgcgcc cccgaaagat tgaagatgga
     1681 gcgtttcaga atgttcgccg ccatgcgcac ggcgtactcc tgccccatga cccgttcccc
     1741 cagaaattcc tcggcggagc gcatggtggc ggggttcagc cgggaccagg gattttcctc
     1801 aatgcccagg cggtagcgcc ggatggcctc cccgatgttt tccaaagaga tgccctcttg
     1861 ccgggcccct tgggcgatgg cccggatctc gctggtaaaa agtccccccg tggcgtcggt
     1921 gaaaagctgc atggcggtgg cgcgcttccg ctcgtcgacg ggggcttcgg cgtcggaggc
     1981 gagggagggg gccagggcgc cgatgagctc ccgccggatg gcggcgtcgg ggcggggcac
     2041 gggaagcacc cgcacccggg gattgtccag ggcgtaccag gggggaaggt cgttttcctt
     2101 gtccagcagc cagaagagca ggttgaaccg gggacggggc aaagctccgg gggaaatccg
     2161 gcggtgcgtc cggggaaggg cccgttttcc cagacggtgc atgcggaaga agaacgccgg
     2221 ggcgtcgtga cggctcaggt cctccagccg cgaggcgcag gagatgacca gggcacaggg
     2281 gaattcggcg caggagacga tccgctcggc gatttccgcc gcccgggtca gcgcggcggg
     2341 gagcacgccc tcgtcggaga gctgctcccc cgtggcggaa aaaaagagcg ccccatcccc
     2401 ctggacgagg gaaaaaccct ccaggggctc gtaccgcacc accaggacgt agccttcccg
     2461 ggccagcagt gctcccagaa attccggcaa gggaggggtg gccacctcgg cccccccggg
     2521 ggggaggggg tagacgtcgt ggacgttgcc ccaaagaaca aactgtggtt tcattgaaag
     2581 aaatcggcct atttcccggg cccatttggg caaaatgccg ccggggttct ccatgcccct
     2641 ctcccctttc cggtgccttc cgcgaggggg cccggggcgg agggcccccg gggatctctc
     2701 cctcgtttcg taaggcgatt ctcttctcga ctccgacgaa agtatagggc tttttattcc
     2761 cccagagaag agtacccatc tatggtgtcg gggtgtcggc gtacaatgga gatcggaagg
     2821 ggaggggacg gaaaccccct cccgggcgga gaacgacttg agaaaagggg gaggggacat
     2881 gggaggcgtc atcttgtcct acggccgggt gaaggacctg ggggccgatg tggccgcctg
     2941 gcgagagggg ggccgacaaa ccatctttct caccccctcg tcggaggacc gggagcttct
     3001 ccggggtctt ctggcgggag agaagggctt ttttggagag ctgcctccgg tgtggcggtg
     3061 gaatgacctc tatcgagaat tggggaaagc ggtccgggtg gccatcaaag gacagctcga
     3121 ccctccggac cacgccctgg tggtggccca cgtggtgcga acccttatca aagaggccga
     3181 tgagaagggg tgggccctgc cccccggggt gcggcggggg ggctttaagg cccttctggg
     3241 gcgccatctc cgggagctcc tgcgggaggg cgtggaaccg gaagatttga cccgggggct
     3301 gaagtgcccg gggtgctccg acgccccctg cgggaacctc ggccatcccg gaggaattct
     3361 ttgccgcttg ttccgggact acaccgattg gctcgcggcc cacgacctgg cggacagcgc
     3421 cgccgtcccc tccctggccg ccgacctcct cgaaaaggag ctccccaaaa agtgggcccg
     3481 ggagcggcgt ttcgtcctgg tgggctttct ttctttcacc tcggcccagt tgcgcctggt
     3541 gcgcgccctg gggagcgtgg cggaggtcgt gctggccaag cccgatccgg gcatcgaagg
     3601 cttttacgac ggggcggagc agttggaagc ctggagccgg gaaaaacggg gggatcccgg
     3661 gggcaagtgg catctgctgg aagcgggaac cccttcgctg gaacccgaag tagcggtgcg
     3721 ggagatcgcc ctctgggccg cggggcggag tgccctggcc tcccgccttt cctggccggg
     3781 cttcggcgga gtgggggtgg tggccccccg ggaagccctt cccctgctgg aagaggccct
     3841 ggggcgctac cgtgtgccct ggagcttccg ggggcgcgtt tccgcagcgg aaaccttttt
     3901 gggcaccctg ccccgccaga tctggcgcgc cgcccagggg gggtggggat tgggggaaac
     3961 ggccctggcg ctggcccacc ccctcttggg gggagatgcc ctgcgggagc gcgccggaaa
     4021 ggaacgcccc tcgggggagg aggcgtggcg ggcctttgat gaccgcgggg cggacaagac
     4081 ctttaaaaaa gtggccgact ttgcccgcat gctcaaagaa ggggcccctc cccgcaccct
     4141 tttggaggcc ctggagacgt tgatttccca ggggaacgaa ggattgcgcc gcgccagcgc
     4201 cctggccgat tcccccctct ggggccccga gggcccccgg catggggagc tggatggggc
     4261 ggtgcgccgc ctggcggggg ccctggagga gctccgccgg aaaatccgct ccctggaaga
     4321 gagggaaaaa ctcctggaag aggcggggaa ggcctttttc caaggggtgg acgccctcga
     4381 ttttctggaa gagtgggcgg aagaagccac cgtggcggcc gcgcccccct tggggggagc
     4441 ggtggcgctc tacgccgaca ctccgccggt gctggcgacc catccggtgc tcttctttct
     4501 ggggctcacc gcgtcccggt ggcccggtac cggcgccgac tccccccttc tgggggatga
     4561 cctccgccgg gaagtgaacg ccggagagcg tgacggagct cccgcgttgc acctcctgct
     4621 ccggcacgag cgacggcagc agaaagaagc cctctttcgc cgcattgtgg cggtgggaga
     4681 acagctgacg gtcctttccc gcccccaaca ggacagcgag ggacgaccca cggccccctc
     4741 tccctttgtg gaatccttca cccgaaactc gaaggatgcc gccggagaaa ataccctcgt
     4801 ccgctccgcc gccgaaaccc tccccgacgc ggaggagccc ttctttcccg cccgggaagc
     4861 cctccggcgg gggaagatcg ctttccgggg gaactttccc cgcaccgcct cctgggagcc
     4921 ccggaaccgg ggaaacttca gtgacctgga tcttttcacc gcctgtccct ttgcctacag
     4981 catggcccgc ctgcgaaagc tggaagagaa atcatgggag gaggaggggg gaggcgaacc
     5041 cctccgggag gggaccttcc tccaccgact ctgggagctg gtgtggcaga cccccggagc
     5101 gggggaggag ggaacttctt tagaagagct ggtggaccgc tactgggacc aagctctgga
     5161 gcccgaagag ggggcttcgt ccggccatcc ctacgaaggc taccgggcgc tccgggaaga
     5221 tccccgatgc gccccccggg cccgctttct ccgcttcgcc gctcgccgcc tggggcgcct
     5281 tcaggaccag gccgaggaga gccgtcgcag agacggcgtg gaggtgacac atcgcctgcg
     5341 ggagagcgtc aacccccgca tggtcgtggg cggggtgacc ttctccggac gctgcgaccg
     5401 ggtggacctc acctcccagg gagccctttt gctggactac aagctgggca acgtggcggg
     5461 gaaaaaaatt cggcggctcc agctggcggc ctacgctctg gccctggaag aagggcggtt
     5521 gtccggggaa aaccccctcc ccggcggcca ggagattcgg ggcttcgcct atctggggca
     5581 ccgagacggc aaggcccagg gggagatgga gcccccgttt taccgctggt acggcatgaa
     5641 atcctccgga agcctcgacg tcgccatggc tgaggcaaga gaagccctgg agagcatggc
     5701 cggagccctg caagagggca aatatccggc gaactacgcg tcggaaaact gcgcctggtg
     5761 cccctatccc gccctgtgtc gccgggggga aagccaggga gaagggatgg gggaggacca
     5821 gggggacgaa gacgaaatcc aagaaggggc gtaatgacat ggtcaccggg ggcaccggcg
     5881 ccacgccgga aaagcacaag aagaaaggag attttcatga taaagaatgc ccaggatgtt
     5941 ttcctgcggg gctttcgcga cgaacaggcc cgggccatca cgtcttcgga gcccctggtg
     6001 gtggtgagcg ccggagcggg cacgggaaag acccagaccc tggccacgcg ctttgcctgg
     6061 cttttggcca cggaccccgc ctgccgggtg gagcagatcc tcaccctcac cttcaccgaa
     6121 aaagccgccc gggagatgca ggaacgcatc cgaggtcggt tggaagagtg gtaccgaggg
     6181 gaccccacgg ggatggccca cctgcgggac gccctggaac gcttcgacga ggggtacatc
     6241 tccaccatcc actcctttgc ctaccgcgtg gtgcgggaat cggggctggc catggacctc
     6301 gatccggggg ccaccctcct ggcccctccc gaagaagagg ccttttggga ggaattcacc
     6361 gccgaactgg agggaaaccc ctcttcctgg gtggccgaag gcctggatga cccatggggc
     6421 ccccgggccc gggagttctt cgcctcggcg gacttcgtcg acctgcggga cggcttcggc
     6481 gcctccgccc tggcctccgc cgtgcgggaa acctcggaac tcgccgccag cctggggtgg
     6541 aaccccgaaa aactttggga ttggagcgac gacgagaccc tggaaaccct aaggaagatg
     6601 gtggacccct tcgcccggga gtgctgggag ctgtggcagt gcgtcctgat ccccgccgtg
     6661 gaatccctgc tggcccaaga caccacccga ctggggaccg ccctgaaaaa cctcaaggaa
     6721 cgcttccgag gagaggaagc tccggatcga gacctcatgc ggcagttttt cgccgcgatc
     6781 cacgacgccc tggaaaaact cgatggggcg ggcaaggtga aaaaactcct ggaagacact
     6841 ctgggagaag ggctgaaatc ctggcgtgac accagaaaag agatgatgct cctggcccgg
     6901 gagctcacgg ccgacgcccc cgagcctccg gaagaaaagc gtctccggca gggacttcgt
     6961 tgcctcgccg ccctgggttg ggagcgctgg gatcggtggc gtcgcctccg gggaggactc
     7021 atcttccggg acctcatcgc cggagctacc gaagtactgc aaaaagaccc ggaagcggca
     7081 aagcgcttcc gacacatcat ggtagacgaa tttcaggata ccgatcccct gcaggacgcc
     7141 ctgatccggg ccctgtggcg tcccgacacc aacaccctct ttctggtggg ggacctcaag
     7201 cagtccatct accgctttcg tcacgccgat ctcacccttt ttggcacgta catcgacgaa
     7261 gccaaaaaga cccccggaga ttcccgggag agctacatcc ccctgccgtg cagcttccgc
     7321 ctccggtcct ccctgctgga cgaagtcaac gccctctttg gtgacatctg gaaagacggg
     7381 ctggggaaag accttcccct gccctacgag gccctcaccg gccccgaaga tctgccgttc
     7441 tggaaatgtc gcaacgcctc cggcggagct cccgtggagg cgctcctggc ccagacctcc
     7501 cggaaggcca agggagagga gacgtcgcgc cacggcaccg cctccgaggg gagggcccgg
     7561 tgcttttccg ccctggcggc gcgtttccgg gaaatccacg accgcccgca aggggaagaa
     7621 agcctcgaag accgagtgtg ggacaaagac ggggcgtgct ggcgcgcccc ccgatggagc
     7681 gatttcgccg tgctggtgcc ctcccggggg cactaccccc ttctggagca ggccttcgac
     7741 gctgcgggca tccctgtggc cttcgacggc agccgggcgt acttctcccg gggcgaagtg
     7801 cgggacgtgg tgaacctcct caaagccctg gcgcgccccg aagacgacct ggccctggcg
     7861 gggtggagcg cctcgccctt ttccggcgtc ctgccgcacc agaccctgga gtggctctcc
     7921 cccccggggt cccggcaaag cctgacggct cacgccgaag ccccgacgtg ccccgaaaaa
     7981 atccaaggac ctttgtccgg ggcctcgtcc cgggagacct catcggaatc cttccccccg
     8041 gggtccctgc gggaccagat ggcccgtcat gcccccgccg ccgcccggcg cttcgaccgc
     8101 ctacgacgca ccgctcgact caaaggagcc tcgggggccc tggctctcct cttggaagat
     8161 ccccacgccc tccgagcctt cgggccgagt gcccggcgcc gggtggcggc gaacctccgc
     8221 gccgccgagg ccctggcccg ggagtacgaa accaccctgg ggggagagct cggggcctgc
     8281 gcggcctatc tggaccgggc cctccgaaag gagcaccccc gggaggaacc gaccttcgtg
     8341 ggagaagacg aagacgccgt gagggttctc accatccacg gcgccaaggg attggaatac
     8401 cccttcgtgg ccgtgacggg gctggagcgg agccccggaa gggagaaaac ggcgacgatc
     8461 accccctccc gccacctggg ggcggtcttt tcccgcatcc cccccctgct caaaggcgcc
     8521 cccgcccagc cccccgccgg gagcacctct gccgggagcg ccggcttcca gccggctcat
     8581 tcttccccat cccccgacat gcccgcagag atcgcctcaa gcagggggaa acaacgtgac
     8641 accggcgaaa cctccgccaa atccgaagaa agagacaccc ccaccaccgc gtcccgatgg
     8701 catcgcctcc tggaacgcca ggccctggtc gaagaaaact ggcgcctctt ctacgtggcc
     8761 accacccggg cccgggaaaa gctcttcctc tgcggcaaca tctccatgga caaagacgac
     8821 gccgccgccg ccgcccccaa ggactcctgg ctgggccccg tgcgccaacg cctgggagaa
     8881 ggtgggactg cggtaacctg tcgcatcctc tctcgccagg acgaagaaat cctgaaatcc
     8941 ccggggaaaa accccgaaat ggccccttct ccggcgccgg cggatgccct gcccctgccc
     9001 tctcccgacc cctttttggg gcgcctcggg gcctcggcct acgccctcct gcgctactgc
     9061 ccccgggcct atcgccggtc gtaccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnngcctc ggcctacgcc ctcctgcgct actgcccccg ggcctatcgc cggtcgtacc
     9241 gccaaggaat ccccctgatc tgggacaaaa aagacgatgg ccccggggga agcctcctcg
     9301 gcaccctggc ccacgaggtc ctccgggggt ggaactttgc cgaagaagag ctggagagct
     9361 ggctccccga agacgacgaa gcgcgggaac aggtggcccg gcgccttccc cccgaccttc
     9421 gccttcccct ggggcgggcc gaagaccggc gcgccctggc ctcctggctc cgggcccttt
     9481 gccggagcgc cgaaggggcc aggctccggg ggcgaaccct ccggaaagaa gtggtctttc
     9541 gccttcctct gggccctcgg ggagggggac cgacggtgga cctcgtgggg gctctggacc
     9601 tcctttggga agacgacaag gggctccacg tccgggacta caaaaccgcc cccccggaga
     9661 gcatccccga cgacctctac cgacaccagc tcctgttcta cggtgtggtg gcgcggcact
     9721 gccgcccggg ccgccccgtg gacctcgccc tgtaccacct ccgggccttc gccggagaag
     9781 gaggagatcc tgtgactgcc gtaacccccg ccccctcccc cgaagcgtgg gaggcctggg
     9841 acgatggaga gtgggaagcc gcctggtgcc aggtggaaga agagctctgg caagccgccc
     9901 gacaagccgc ccaaggcccc tggccccccc gccccgaggg gtgccaccga tgcccgtgga
     9961 agagctcctg cgctccggaa taagaatggg ggaaaggtgc cgatggccgt cggggcgaaa
    10021 aaaccttgcc cccattaccg acgggatttt ttcgtgatag ggtggtgaaa gacaacgctg
    10081 gttaaaatat gtaggggcga aaaatttttc gcccctacag gtttcaccct gcatcacctt
    10141 ttccctcgcc gggaaccccc tgggcgtgcg agctgggagc gccggcttcc agccggctct
    10201 tttttaactc gtgctcctca tcgcctctga gctcccttgg cagcacgcca ccgtaaggcc
    10261 accccccctt ttttttaccc ccacccccga cgggattctc gtcgtggcga acgcattttt
    10321 cgcccctaca acccatggcc gtggtggccg cgccgccggg agtccccaca actaatgggc
    10381 gcacagctgg gagcgctcat cttccagccg gctctttttt aactcgtgct cctcatcgcc
    10441 tctgagctcc cttggcagca cgccaccgta aggccacccc cccctttttt tttaccccta
    10501 cccccgacgg gattctcgta ggggcgaacg tatttttcgc ccctacaacc catggccatg
    10561 gtggtcgcgc cgccgagggc tcaccccttc tcgtcgactc tttacaaacc atcgaacgcc
    10621 ccgacaacgt tcacccttca cttcacaaac tctcttcttg taactccttc catcgccacc
    10681 gacgttttcc ttcgcccccc catttccatc gccccccctt gcatctttag gacaagggga
    10741 ataaaatagg tcctgcggaa tatttatctt gcagcggtgt tgggtgcggt acaaactttg
    10801 catggaggtg gttttagtga agaaggttct tttggctttg gcgcttttgg gggtgttcat
    10861 ggcgggggcg gcgtttgcct ccggacacgt ggatccggcc ccttttaagc ccatcgcggt
    10921 ggattcgggc gacgtgcccc tgcccacggg ggacggggga gtcgtgtcca tggacctgac
    10981 acccgcagtg cagggtgatg tcccccagca gggcatcatg gagctgatgg gaggtggcga
    11041 aacggcccac atcctttccg ccgtgaagct ggagcttgtc tataacaacg ccgatggaga
    11101 agcgatcatc gacctgacgg ccgtcatcgc taaggtgaag gcagccacgg ggactgagcc
    11161 ctcggtggtg tggatcacca acaagaaaac gggcaaggtc acggagttcc ccatcagtgg
    11221 tggaaagatc accgtcaagc ctgtggggga ttacttcacc gaatccgtcc tcacctttgg
    11281 caataagacc acggcgagct ccggcagcgg tggcggcggc ggttgcaatg cgggcttcgc
    11341 ccccgccatg cttctcctcg ccctgcccct ggctcttttc atccgtaagt aggaccgcga
    11401 ctccgtaaca cctctgttcc gcagcgggga agaccggcgt ccggtctttc ccgctttttt
    11461 tcagcgacgc ccccccaaat acgccacttt cgggaggtcc tcagcatgcg ccacgggctc
    11521 gtcctttttg tcggtctcct cggggccgtc ctgtcgggaa gcttcctctc gccggcccgg
    11581 gccctctctc ccggcaatgc gggctatgtg ggcctctggg aataccccac cgcccagatg
    11641 gccgaagacg gtcacgtctg ggtgggatat gcggacagcg atccttacct gacatacttt
    11701 gtcaacatgg cctactttcc ctggctggaa gtcaacctgc gcctcacggc cttttccaca
    11761 gctccccgca tctccgaagg gtatggccgc tacaaagaca aggccctgga cctcaaggtc
    11821 ctcctccgga aaggccgggg cttctcccca tcgctggcgg tggggatcac cgacctcatg
    11881 ggcaccgagc tcctccgggc caaatacggc gtcttcacct ggacctggga cagcctcgcc
    11941 ttttcgttgg gctacggcac cgaccgcctc aacggggtct tcggcggcat cgaatggcag
    12001 gccgccccct cctgggcctt caaactcgaa tacagccccc tgtattatcc gggggataaa
    12061 gtctccggca cccggatcca ctccgccccc gccgcctcgt cctggaacgt gggggtggtc
    12121 tacgacgccc cctggggcgg cgaggcctcc ctcagctacc agcggggaga agatctctgc
    12181 ttcagcgtgg cctacgccta cgacctggcg ggaccctttc tgggttcccg agaaaaacgt
    12241 ttggccccct ccaccccccc tccctccgtg tccgactggg acgccgaaga tctgaccctt
    12301 ttggccgaag agctccggcg aaatgtgggg gccctggggg gcctgcggga cgtgcgggtg
    12361 gcggtggggg accacaccct tctggtggcc ttcgaaaaca cggggtacgg ctctgcggcg
    12421 gaggcctgcg cccgggtcct cggcgccgtg gcctggcggg ccccctggga tctccggacc
    12481 ctctccttgc tccccctcct ccgggggcag ccctctgtcc gcatggacat ccccggcagc
    12541 cactgcgccc ttttgcgcct gggagacctc tcccgggagg acagcgccgc cctcgccgtc
    12601 cggtgggcca ccctggacga cctcgcttcc gagggaggcg ccccctggcg ggcccaggcc
    12661 ccacgggagg gctggcctgc gccgggcacg gaattccgtt ttcgcttttc ctacgaaccc
    12721 cgcatcgacc gcatcctcga caacgactac gaagaccgct tcaacctcga cgccgaactc
    12781 cgccaccgca cctcctgggg gtgggagttc tacggcagcg tccgcgtgcc catcaagaac
    12841 aacatcgaca tctggtggga gcccgaaacc aacgatgacg tccgcatctg gcagaccgtc
    12901 ctctcctggg gcaccctgcg cgccctggac gaacgccggg ccgtggccct tctcgccgaa
    12961 gtgggatggc tcgacgccaa ttggttcggt gccaacctct gggggcgggc ctatttcgac
    13021 ggcgggcgct ggtggctcgg aggccgggcc agcgtggtgc agcagcgcga tccccattcc
    13081 ttcggaggac tctcggagta ctggcgcacc tacgacccct caggaggatg ggtcatcggg
    13141 ggccccaaaa acgaatggac cctcggggcc tgggcccagg ccgggtaccg ggagcccacc
    13201 ctggatgtgg atctgctggc ggagtacggc cgcttcctcg acgaggactg gggagggcgg
    13261 gtggcggtga cccgtcactg gaacgacgtg ggggtggggt tctggatgag ccgcaccgat
    13321 atcctcactc cgggaaagga tttcaccaat gcggggatgt atctggagat ccccgtggac
    13381 gcctggttcg gcacccggag cccccacgtc tggacccagg agttcaccct gctctccacc
    13441 tgggcagatt tcgccggacg tcagcccgga gcctggcgca cccccgaacg cctctggggc
    13501 caggtgcttc ccgaccgtct tcccggccag atgtacgccc ttttggagcg gttttgccgc
    13561 gcggtgggga aaaacgccct gccggagttt agcagcaagg cccaaagcct cctggagtgc
    13621 tttacacccg gatcttcctg gggcgaagac gaaacggagc tctattgacc ctagggagcc
    13681 tttcggagaa aatcatcata ggggcgaaaa atctttcgcc cccacaccac cggaaattcg
    13741 taggggcgaa aaacctttcg cccccacacc agtttgagat cagcccccga tctcccaaag
    13801 aaagcgagtg ttgatgcgag catgacgtct cataccctgt tccgccgatg gagtttccgc
    13861 gcccttgtgg gtcttttggc cgccctggcc gtcgccggcg cggcgtggtc ccagggcccc
    13921 accgcccccg gagcccccac cccggtttta ctggaaggcg ccgccccgga gggaggagcc
    13981 cccggaactc cggccctccc ggaaggcccg gcagcaccgg agccggggga ggtccccgcc
    14041 gagggtaaag gaagcaagac tcccgcccct gccgaggggg aggaagtgaa catccaggcc
    14101 cctctggaag ggtcctcacc gccggtaagg cggggcgaga tcccctcatg ggccttcccc
    14161 gcttcggagc ggactttttc cggcgtaccc cctcggtctt cgcccccctg gacaccacag
    14221 cgggggatcc ggggtacgtg gtgggtcccg gagacgaagt gcgcatcgac ctcttcgggg
    14281 cctttaaccc caccggggcg gaggacgcgg ggggcaactg gaccgtcacc gtggctcggg
    14341 atggttccct cgccctcccc aaggcgggca ccgtccgggc ggcggggtcc accctggagg
    14401 gtctccagca aaagatccgg gacgcctacg gcacgtacta caccgatttc gaactccatg
    14461 tgacgttggg ccgggcccgg gccttttcgg tctacgtggc gggggacgtc aaatcccccg
    14521 gagtctacgc catgcccgcc cgggccaccg tggccctggc gctcgccacc gccggggggc
    14581 cgagtccggc gggatcgttg cggcatatca aggtcatgcg gggaggaaag gcggtgggga
    14641 tctttgacgc ctacgacctg ctcctgagcg gcgaccgcaa gggagatctc cccttgcagg
    14701 acggcgacgt ggtcttcgtc ccccccgtgg gcattttggc cgccattacc ggggatgtgt
    14761 tgcgccctgc ggtctacgag ctccggtccg aaatgcccct ggggagcttt ctccagcttg
    14821 ccggagggcc caccagccag caattttccg gccgggtgca gatctaccgg gtggaaaacg
    14881 cccagtaccg catggccttt gaaagcgaag ccgatccccg ggctctggcc tcggtgcaga
    14941 ttcgtcacgg cgacgtggtg aaatttttcc ccatttccgt ggccctctcc gacgtgaccc
    15001 tggccggtgc cgtggcatc
//
LOCUS       DAOOER010000044        14821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_4627_length_14821_cov_8.275836, whole genome shotgun sequence.
ACCESSION   DAOOER010000044 DAOOER010000000
VERSION     DAOOER010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 14821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14821
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4627_length_14821_cov_8.275836"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..2415
                     /locus_tag="PL162_06680"
     CDS             115..2415
                     /locus_tag="PL162_06680"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HQF91739.1"
                     /translation="MSLRARFVLLAAVLCGALAVLVALSSIQLGQILEDQLGVMGKEA
                     AQEGAVTVTQYLERFKTLALSVGAVACRLWEKGALSSALDLEPYLQDVFASQRPRGVS
                     NVYVALELDGTYAEGKGWRAPDSYDARVRPWYRKAVRYGGPVVVDPYMDLGINAPVLS
                     VVTPVRDARGRLVGVAGADVNLKALATLVKDRRTMGEGFSFLVNEDGDFLVGPRDSWA
                     LRERINVSSDAVTGEMARFGIAMLQNRRGVGELTIDGAPYRVFHLRTPDGLKFSQAFP
                     MSSLRSKVAATTRQYALFSLGFAALILGVVLLLLRGIRQSLRGLLEATREVGQRLSPR
                     ADLERTAFSLNEVMWQVWHQAEASKVSDVRVALGHLASVLGVLARQQQELTAYGEETY
                     AMNEALTDNNRVLQQREATWARVLDIARTAIASDRFSEGVRHMAEVIQEATGAFGVAV
                     VRLEEGGLCHVSGAGYQNGVTLESLVPLEGSVAGRAFRLRETQWVNSVQDEKEYFLLH
                     PRVMTEVEIPLIQAGEALGVVEVAFDTPRPYDPDMLELLTPITSALAGFLHGEKAHRE
                     VRQSYNYLAERLLVITGIYHDETAGHLQRMGGYCHLLASEWGRSEEEQGRIALFARLH
                     DIGKVRVPMQILGKPGPLTKEEFDTVKKHPEWGAEILGDAAWLAMARQICLTHHEKWD
                     GSGYPRGLRGEDIPWEGQVTALADIYDALRARRVYKPPMSHEEAVRVLLEGDRRVRPE
                     HFNPVLLEIFRHSASTMDQIFRQFDE"
     gene            complement(2618..3967)
                     /gene="gltX"
                     /locus_tag="PL162_06685"
     CDS             complement(2618..3967)
                     /gene="gltX"
                     /locus_tag="PL162_06685"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HQF91740.1"
                     /translation="MTAQVRVRFAPSPTGALHIGGGHTALFNWLWARHTGGRFILRIE
                     DTDRERSTREYEETILAGMRWLGLDWDEGPDVGGDFGPYRQSERLELYRASASRLVDE
                     GKAYCQDGAVYFRIPAGKTIAWDDVVYGPIEVDGDTLRFDTQNTAPKDLVLLKSDGYP
                     TYNYAVVVDDAGMAINTVIRGEDHIANTPKQILLYEAFGWPVPRFAHLPMILGADKKK
                     LSKRHGATSIYEYRDLGYLPDGAFNFLALLGWSPGDENEICSRREAVELFELSRVTKR
                     AAVFDMQKFAHVNQAHLWRTPPEELVELLRPLWKARGLDPDRFDAAWLARALELLGGR
                     GQTTLEVAEYSDYFLSFDAVTARYDGADLPEETRRSLRPFYAGLLELPTWHHGAMEAY
                     AKQWGEEHGAKMKDLAMPLRWVLTGRKVSPGVFDVAVFLGREEVGRRLGHYRLLPEA"
     gene            4153..>4757
                     /locus_tag="PL162_06690"
     CDS             4153..>4757
                     /locus_tag="PL162_06690"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HQF91741.1"
                     /translation="MSEETLLLIDGHGLAYRAFFALPELSAPDGTPTGAVVGFANMLF
                     KILEQWKPSGGALVFDAPGPTFRHEAYEGYKEGRSPMPDLLRAQIPLLHELGELLGIS
                     VVARQGVEADDVLGATALEACARRPVLVVTADKDLFQLLRPGLRVLRPRKGVSEFTIY
                     DEEVFQNEYGFSPDRFREYLALVGDAADHVPGVPGVGDKGAR"
     assembly_gap    4758..4857
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4858..6453
                     /locus_tag="PL162_06695"
     CDS             <4858..6453
                     /locus_tag="PL162_06695"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HQF91742.1"
                     /translation="EVAPEDLSPEGPLALVEVEDKTSGLREFWGGSAEGAWARFIHRD
                     DVGAFCRGLLPEATVVTSRYKELCAALPAGTLPPEKVWDAQVAHYLLHPDRSSREYSS
                     LLPEDPRECLAALWRLQGALAPELERQGLGEVFRTMDVPLCPVLAAMEQHGLGLHVGR
                     FEALERDLGERIALLEGQLRALAGGDLNPNSPKQVAAFLFDRLGLPPQKKIKTGYSTD
                     VAVLEILARLPQGEAPRLLLEHRELAKILSGFVHPFLRARNEATGCVHATFEHTVTGT
                     GRLSSANPNVQNLPAYGEWAKRFREGLVPRTPGNIFVAGDYSQIELRVLAHLSGEERL
                     IQAFRDRRDVHAETAEWLFGPGSGTGESRRIAKMINFGLLYGMGTFGLAERLGIGRRE
                     AALVVERHAETFPRIHAYLHDSAEEARGRGYTRTLFGRRRPLREVVTTEGRGGNALQR
                     VAVNAPIQGTAADIAKLAMVAWAREGRAPLVLQVHDSLVVETSSDRALAVGEELARVM
                     TSAASLAVPLEVEIKRGESLAEV"
     gene            6658..7794
                     /locus_tag="PL162_06700"
     CDS             6658..7794
                     /locus_tag="PL162_06700"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HQF91743.1"
                     /translation="MRTLRTQVKWIMISVIVVFVLTTFWGYGSYSSRSQKGRSAGQDY
                     AVAEVDGREVMRSQLDQGVLDLAERSSSTKDIKEADVVALRRSVLDNVAIASQLEKEL
                     KRSHIEVSDEEVTNAVKDVEDRFPTKESYQEYLEQSGVTEKQIRAQLREDLSRRKLLE
                     NATVGVAISGDEDVKTYDALKPLIFTRRPYVEVQIAAFLSPDRAEAALARMRGGEPWD
                     GVLKDLSGDLRERTSGDATTAFSEGEVPPQIWAAVASADDGAFVGPVSLESRDHYVLR
                     KVSYSPGGALPFDEVSADVREMVLGQKRRAAQEEFLKMLRDRAEVKILDEELFTAPVV
                     SEDLSGDRKSSADQAPSSPDAPAPKDEDPQLSPDAPAPGESSDQ"
     gene            7917..8010
                     /locus_tag="PL162_06705"
     tRNA            7917..8010
                     /locus_tag="PL162_06705"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7951..7953,aa:Ser,seq:gct)
     gene            complement(8578..8663)
                     /locus_tag="PL162_06710"
     tRNA            complement(8578..8663)
                     /locus_tag="PL162_06710"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8627..8629),aa:Ser,seq:tga)
     gene            9217..9630
                     /locus_tag="PL162_06715"
     CDS             9217..9630
                     /locus_tag="PL162_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HQF91744.1"
                     /translation="MTMAEIIERFICIPSTVAVVGASPKEDRSVFSIMSYLHEAGFHL
                     HPINPGYAGESIQGYTCLASLDDLPEPVDVVALFISAEHQEGVVQSLRRLPYKPIVWF
                     QPGSENADQERALAAAGYPVAVNSCMMMIHQVYCH"
     gene            9665..10126
                     /locus_tag="PL162_06720"
     CDS             9665..10126
                     /locus_tag="PL162_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HQF91745.1"
                     /translation="MAGKIVAVCVAPAKGIPKEDVGEGLLVEEEGLEGDAHRGFAHRQ
                     VSLLNAEDIARAREKMPSLGPGSFAENLTVEGFDLGQLKVGDRITVGDALLEVSQIGK
                     ECHTRCAIHHATGDCIMPRQGVFCRVRRGGWVRRNDLVDRAHGAGPGEAPA"
     gene            10123..10569
                     /locus_tag="PL162_06725"
     CDS             10123..10569
                     /locus_tag="PL162_06725"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQF91746.1"
                     /translation="MKSKEAPGRAATVRVRYAETDRMGVVYHSNYLVWFEVGRTELCR
                     SLGFPYRAWEAEGVFLPCVEAHCRYKHPARYDDEVAILTGVKELTPHTVTFGYRVERE
                     GRLLAEGWTKHGFCNPEGKLLREPQPFFGMLGDFLAFSKDTSKREE"
     gene            10585..12426
                     /gene="iorA"
                     /locus_tag="PL162_06730"
     CDS             10585..12426
                     /gene="iorA"
                     /locus_tag="PL162_06730"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HQF91747.1"
                     /translation="MVLLGNEAIARGIVEAGCEVAVAYPGTPSSEILPAVAKYADELG
                     TRTAVEWGVNEKVAFEVAAAASFAGKRSCAIMKQVGVNVAADALMSVAYFDLAGGFVL
                     VAADDPGPHSSQTEQDSRSFAQFAKIPCFDPSSAVEAKHMVFDAFDLSEQYHIPVMLR
                     PAGRVNHCREDVELGAVRPGDHPAKFVKDVRRWVCLPAHVRLTHPQLNAKNDAIRQDF
                     ESRFGAYNYELAAKKPAKLGIVAGGVSFAIVQDLLASWGREDVAVLKIGTPVPLPEKM
                     VMDFISRHDNVLILEETYPVIENQLPDRTKVKGRHNGFVPRAGELLPEVIEELVLRAL
                     GEPGVPGASPELKAALEELQIKPKPPMLCPGCPHRASFFAIRKAVPSGIYPSDIGCYT
                     LGVNQRMVDSVICMGASVTQPSGFYLAHRVDGKEQPLVSTIGDSTFFHMGIPGLVNAV
                     YNRHAFVLAILDNSITAMTGGQPNPNVGTKLRKGDVGARVDIEGAVRGCGVGHVEVVE
                     AYDMASGEKAVKAAWEYAKAHQSPAVVIFRHPCMLLRCEQEKIRVRVDEERCVGCKFC
                     INFFNCPALVFDEGKNKAVVDDRFCVQCGTCIPVCPHGAIVRVSEEA"
     gene            12429..12986
                     /locus_tag="PL162_06735"
     CDS             12429..12986
                     /locus_tag="PL162_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HQF91748.1"
                     /translation="MQYIVVGLGGQGILFSSRVLGHIAVAKGEHVMGSEVHGMAQRGG
                     SVVSHFKVGPYASPLVRAGNADILLAFDQNEGVRNLHFLKPGGKAVINVHKPESLENA
                     HLQAFIKSRNIHIALIPGYDMLRQHMGGNFLFLNVLILGALAGTGMADVSMEEIRQAV
                     AELAPAKARDANLAVLELGYKHFAS"
     gene            13114..13638
                     /gene="lepB"
                     /locus_tag="PL162_06740"
     CDS             13114..13638
                     /gene="lepB"
                     /locus_tag="PL162_06740"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HQF91749.1"
                     /translation="MAAKSWWRETIETILWALALALILRTFVVQAFWIPSGSMIPTLE
                     VSDRVLVAKFWYTFFEPRRGEVVVFKYPVDPTRDFVKRIVGLPGDVVYLKDGVVYVND
                     QALDEAYVKNHDSFNMPSLTVPPGHYFVLGDNRPNSQDGRFWPSGSQFVPKSFIRGPA
                     FFRYWPLDRLGLPY"
     gene            13642..14493
                     /locus_tag="PL162_06745"
     CDS             13642..14493
                     /locus_tag="PL162_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HQF91750.1"
                     /translation="MAGRTVWYPGHMAKGKRALEELASKLDVLLEVRDARAPRLTASF
                     QAQELRRLRPVWVILAKRDLAEEGVTASWLRWLERDGGRAWAMDLRKGRLDYLRRALR
                     EMAPGHREARVAVVGVPNVGKSLLLNQLLGGAHRAPVGGIPGITKGVSWYRVEGNLLV
                     VDSPGILDPKAEAGIQRSLAWLGSSKGEVIGGAESLAVDLLRYLRDHGLWGVVERTWG
                     VCPSEIPEGAEEPDDLDLLGRRLGCLLRGNRVDRELAGRRLLDAFSQGKVGPLSLERP
                     GEGGGGA"
     gene            14490..>14821
                     /locus_tag="PL162_06750"
     CDS             14490..>14821
                     /locus_tag="PL162_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HQF91751.1"
                     /translation="MIVAGTDEAGRGPLAGPVVAAAAVLEPFQMRELMGLGLRDSKRL
                     SPRRRDIIFASLGLLGVVWRAQAASPARIDRDNILQASLWAMGRAVLSLPERPHLVVV
                     DGTVEIPG"
BASE COUNT         2558 a         4369 c         5095 g         2699 t
ORIGIN      
        1 ccctttcgtt tcccccgggg gcgtggtact atgcgtgggg gcatggcgac cagatgctgg
       61 ggaggaagac cgggtgtcgg tcttcggggc acctgcccgg agagggggct atcgatgagc
      121 cttcgtgctc gttttgtcct tttggccgca gtgctctgcg gggcgctggc ggtgttggtg
      181 gcgctttctt cgatccagct cggacaaata ctcgaagatc agctgggggt catgggtaag
      241 gaagcggccc aagagggggc cgtcacggtg acccagtacc tggagcgctt taagacattg
      301 gccctgagcg tgggggcggt ggcgtgtcgc ctgtgggaaa agggggccct gtcttccgcc
      361 ctggatctgg agccctacct gcaagacgtc ttcgcgtctc agcgcccccg aggggtgtct
      421 aacgtctacg tggccctgga gctcgacggc acctacgccg aaggcaaggg atggagggcc
      481 cccgattcct atgacgcccg ggtgcgcccc tggtaccgca aggcggtgcg gtacgggggg
      541 ccggtcgtcg tggatcccta tatggatctg ggcatcaacg ccccggtgct gagcgtggtt
      601 accccggtgc gcgatgcccg gggaaggttg gtgggagtgg cgggggcgga tgtgaacctg
      661 aaggccctgg cgacgctggt gaaggatcgc cgcacgatgg gggaagggtt tagcttcctg
      721 gtgaacgaag atggggactt cctggtgggg ccccgggatt cctgggcgtt gagggagcgc
      781 atcaacgtct cttccgacgc agtgaccggg gagatggccc gtttcggcat cgccatgctc
      841 caaaaccgcc gaggagtggg ggagttgacc atcgacggtg cgccttaccg ggtctttcac
      901 ctccggaccc ccgacggatt gaagttctcc caggccttcc ccatgtcatc cctgaggagc
      961 aaagtcgcgg cgaccacacg gcagtacgcc cttttttccc tgggctttgc cgccctgatc
     1021 ctaggggtgg tgctcctgct cctgaggggg atccgccagt cccttagggg ccttctggag
     1081 gccacccggg aggtggggca gcgcctctcc cctcgggcgg acctggagcg cacggccttt
     1141 tccctgaacg aggtcatgtg gcaggtgtgg caccaggcgg aggccagcaa ggtttccgac
     1201 gtgcgggtcg ccctggggca tctggcgagc gtgctggggg tgctggcgcg ccagcagcag
     1261 gagcttacgg cctacgggga agagacctac gccatgaacg aagccctcac ggacaataac
     1321 cgcgtgttgc agcaacgaga ggccacctgg gcccgggtgc tggacatcgc ccgcaccgcc
     1381 atcgcttcgg accgtttcag cgagggggtc cgccatatgg cggaagtcat ccaggaggcc
     1441 acgggggcct tcggagtggc ggtggtgcgc ttggaagaag gagggctctg ccacgtctcc
     1501 ggcgcgggtt atcaaaacgg ggtcacgctg gaaagtttgg tgcccctaga gggctccgtg
     1561 gcggggcggg ccttccgcct gcgggagacc caatgggtga actccgttca ggatgaaaaa
     1621 gagtactttc tcctgcatcc ccgtgtcatg acggaggtgg aaatccctct catccaggcg
     1681 ggggaggcgt tgggtgtggt ggaagtggcg tttgataccc cccgccccta tgaccccgac
     1741 atgctggagc ttttgacccc catcacctcg gcgctggcgg gctttctcca cggcgagaag
     1801 gcccaccggg aggtccgcca gtcttacaac tatctggcgg agcgcctgct ggtcatcacg
     1861 gggatctatc acgacgagac ggcggggcac ctccagcgca tgggaggata ctgtcatctg
     1921 ctggcgtcgg agtggggacg gtcggaagag gagcaggggc gcatcgccct ctttgctcga
     1981 cttcatgaca tcggcaaggt gagggttccc atgcagatct tggggaagcc cggccccctc
     2041 acgaaggaag agttcgacac ggtgaaaaag cacccggagt ggggggcgga aattttgggc
     2101 gatgcggcgt ggttggccat ggcccggcag atttgcctca cccaccacga aaaatgggac
     2161 ggcagcggct atcctcgtgg tcttcgggga gaggacatcc cctgggaggg gcaggtgacg
     2221 gctctggcgg acatctacga cgctcttcgg gcccggcggg tctacaagcc ccccatgtcc
     2281 cacgaggaag cggtgcgggt gctgctggaa ggagaccgtc gggtgcgccc ggaacacttc
     2341 aaccccgttc ttttggagat cttccgacac tctgcctcca ccatggacca gatcttccgg
     2401 caattcgacg aataaatttt tgggaaaacg gcagacgccg gaacgctccg ctgtcttctg
     2461 cggagatgtt ccggcgcctg ccatgttccg cgcaccctga cggtgctgta ggggcgaaca
     2521 accttttgcc ccgcaaggac tgtgccgtcg gggtgaaaaa tccttccccc ctcaagggcg
     2581 gcgctgtagg ggcgaaaaat ctttcacccg gcgacgctta cgcttcgggg agcagacggt
     2641 agtgccccag ccgccgcccc acttcttccc gccccagaaa gaccgccacg tcaaagaccc
     2701 cggggctcac cttccgcccc gtgagaaccc aacgcagagg catggccaga tccttcatct
     2761 tggccccgtg ctcttcgccc cactgtttgg cgtaggcttc catggcgcca tggtgccatg
     2821 tgggcagctc cagcaacccg gcgtaaaagg gacgcaggct ccggcgcgtc tcttccggaa
     2881 gatcggcgcc gtcgtatcgg gcggtgactg cgtcgaaaga caggaagtag tccgagtact
     2941 ccgccacctc cagcgtggtc tgcccccgtc ctcccaggag ctccagggcc cgggcgagcc
     3001 atgcggcgtc gaagcgatcc gggtcgagcc cccgggcttt ccagagggga cggaggagct
     3061 ccaccagctc ttccggaggc gtgcgccaga ggtgggcctg gttcacgtgg gcaaattttt
     3121 gcatgtcgaa gaccgctgcc cgtttggtga cccgggaaag ctcgaaaagc tccaccgctt
     3181 cccgccggga acagatctcg ttctcgtccc cgggggacca tcccaggagc gccagaaagt
     3241 tgaaggcccc gtcggggaga taccccagat cccggtactc gtagatgctg gtggcgccgt
     3301 gacgtttgga gagttttttc ttgtcggcgc ccaggatcat ggggaggtgg gcaaagcggg
     3361 gcacgggcca gccgaaggcc tcgtagagga ggatctgctt gggggtgttg gcgatgtggt
     3421 cctctccccg aatgacggtg ttgatggcca tgcccgcatc gtccaccacc acggcgtagt
     3481 tgtaggtggg gtacccatcg ctcttgagaa gcaccaggtc cttgggggcc gtgttctggg
     3541 tgtcgaaccg gagcgtgtcc ccgtccacct cgatggggcc gtacaccacg tcgtcccagg
     3601 cgatggtttt ccctgcgggg atacggaaat acaccgcccc gtcctggcag taggcctttc
     3661 cctcgtccac caggcgggag gcggacgccc ggtagagttc cagacgctca gactgacggt
     3721 agggaccgaa gtctcctccc acgtcggggc cctcgtccca gtcaagcccc agccagcgca
     3781 tgcccgccag aatggtttcc tcgtactccc gggtggaacg ctcccggtcc gtgtcttcga
     3841 tgcggaggat gaagcgcccc ccggtgtgcc gggcccagag ccagttgaaa agggcggtgt
     3901 gtcctccgcc gatgtgcagc gcccccgtgg ggctgggggc gaaacgtacc cgtacttgtg
     3961 ccgtcatgtt gaccctccta gtggcggtgt ttgtcgatac cggggaaagg ggcgagcttt
     4021 tcctgggccg cccgaggcga taaatcggtt ttttccccga gatcgtgcta gagttacgag
     4081 gtaatctttg cccgaaatcg gcaaagggag atgggggtag tataccgcac ccccgggaaa
     4141 ggagcacggc ccatgtcgga agaaacgctg ctactcatcg atggccacgg cctcgcctat
     4201 cgggcctttt ttgccctgcc ggagctgtcg gcccccgacg gcacccccac gggggctgtg
     4261 gtgggctttg ccaacatgct tttcaagatc ttggagcagt ggaagccctc cgggggggcc
     4321 ttggtcttcg atgctccggg gcccactttc cgccacgagg cctacgaagg ctataaagag
     4381 gggcgatccc ccatgcccga cctcctgagg gcccaaatcc ccctcctgca tgaactggga
     4441 gagctcctgg gaatttccgt ggtggcgcgc cagggggtgg aggccgacga cgttttgggg
     4501 gccaccgccc tggaggcctg tgcccgtcgc cccgtgctgg tggtcacggc ggataaggac
     4561 ctcttccagc tcttgcgccc cggcctccgg gtccttcgcc cccgtaaggg ggtttcggag
     4621 ttcaccatct acgatgagga agtttttcag aacgagtacg gcttctcccc cgaccgattt
     4681 cgggagtacc tcgccctggt gggggacgcc gccgatcatg tccccggcgt gccgggggtg
     4741 ggggacaagg gggcccgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngag
     4861 gtggctccgg aggacctttc cccggagggc cccctggccc tggtggaggt ggaagacaag
     4921 acgtcgggcc tgcgggagtt ttgggggggc tccgccgaag gggcgtgggc ccgctttatc
     4981 caccgggatg acgtaggagc cttctgccga ggcctcctcc cggaggcgac ggtggtcacg
     5041 tcccggtaca aagagctctg cgccgccctt cccgccggaa cccttcctcc ggaaaaggtc
     5101 tgggacgccc aggtggccca ctatctgctc caccccgacc gttccagccg ggagtactcc
     5161 tccctgctcc ccgaagatcc ccgggagtgc ttggccgccc tgtggaggct ccagggcgcc
     5221 ctggcccccg agctggaacg tcagggactc ggcgaagtct tccgcaccat ggatgtgccc
     5281 ctttgccccg tgctggccgc catggaacag cacggcctag ggctccacgt ggggcgcttc
     5341 gaggctctgg agcgggacct gggggagcgc atcgccctcc tcgagggtca gctccgggcc
     5401 ctggcggggg gggatctgaa ccccaactcc cctaaacagg tggcggcctt tctcttcgac
     5461 cgcctgggcc ttcctccgca gaaaaagata aaaacgggat attccaccga tgtggccgtg
     5521 ctggagatcc tcgcgcggct cccccagggg gaggctcccc gcctgttgct ggaacatcgg
     5581 gagttggcca agatcctttc cggcttcgtc catcccttcc tgagggcccg aaacgaagcc
     5641 acggggtgcg ttcatgccac cttcgaacac accgtcactg gcacggggcg cctttccagc
     5701 gccaacccca acgtgcagaa cctgcccgcc tacggcgagt gggcgaagcg cttccgggaa
     5761 ggtctcgtgc cccgcactcc gggaaacatc ttcgtggcgg gggactactc ccagattgag
     5821 ctccgggtgc tggcccacct ttcgggagaa gagcgcctca tccaggcctt ccgggaccgg
     5881 cgagacgtcc acgccgagac ggcggagtgg ctctttggcc cggggtccgg caccggcgaa
     5941 tcccggcgca tcgccaagat gatcaatttt ggtcttctct acggtatggg caccttcggc
     6001 ctggcggagc gtctgggcat cggccggagg gaggcggctc tcgtggtgga gcggcacgcc
     6061 gaaacctttc ctcgcatcca tgcctatctt cacgatagcg ccgaagaggc ccggggccgg
     6121 gggtacaccc gcaccctctt cggccgtcgt cgccccttga gagaagtggt caccacggag
     6181 gggcgaggag ggaatgccct ccagcgggtg gcggtgaatg cccccattca aggcactgcg
     6241 gcggacatcg ccaaactcgc catggtggcc tgggcccggg aggggcgggc gcccttggtg
     6301 ctccaggtcc acgactccct ggtggtggag acctcctccg accgggccct tgccgtggga
     6361 gaggagctgg cccgggttat gacctcggcg gcctccctgg cggtccccct ggaggtggaa
     6421 ataaagcggg gggagtccct ggcggaggtc tagggcccgg gccgggaggt ttttttcgga
     6481 ccgatcgggg gaggtcgccg caaagagaag gggtaaaatg agatcggctt aaaaagcggg
     6541 aagtgaggcc cgggaggagc ggactccttt tgacagggac gcttgcccca cgtatgatac
     6601 aatacgccgt cgggaagaac cgacgcgaaa gaggaaaggg gtcgatgggc gttgttgatg
     6661 cggaccttgc ggactcaggt gaaatggatc atgatcagcg tcatcgtggt cttcgtgctg
     6721 accacatttt ggggatacgg ttcttattcg tcccgttccc agaagggacg gtcggcggga
     6781 caggactacg ctgtggccga ggtcgacggc cgcgaggtaa tgcgctccca gcttgaccag
     6841 ggcgtgttgg atctggcgga gcgatcctcc tccaccaaag acatcaagga ggccgacgtg
     6901 gtggccctgc gacgctccgt gttagacaac gtcgccatcg ccagccagct ggagaaggaa
     6961 ctgaagcgga gccacatcga ggtttccgac gaagaagtca ccaatgccgt aaaagacgtg
     7021 gaagaccgct tccccaccaa ggaatcctac caggagtacc tggagcagtc cggggtcacg
     7081 gaaaagcaga tccgggctca gctccgggag gacctctccc gaagaaagct tctggaaaac
     7141 gccacggtgg gggtggccat ctccggcgac gaggacgtca agacctacga tgccctgaaa
     7201 ccactgatct tcacccggcg tccttacgtg gaggtccaaa tcgccgcctt cctatcgccg
     7261 gatcgggcgg aggcggccct ggcgaggatg cgcggcggtg agccctggga cggggtgctc
     7321 aaagacctct ccggggacct gcgggagcgc acctcggggg acgccaccac cgccttctcc
     7381 gagggagagg tgccgcccca gatctgggcc gccgtggcct cggcggacga cggcgccttc
     7441 gtgggccccg tgtccctgga gagccgagat cactacgtcc ttcggaaggt ctcctattcc
     7501 cccggcggcg ctcttccctt cgacgaggtg agcgccgacg tgcgggagat ggtgctgggc
     7561 cagaagcgac gggcggccca ggaggagttt ctcaagatgc tccgggatcg ggccgaggtg
     7621 aagatcctcg acgaagagct cttcaccgcg cccgtggttt cggaagatct ctccggagac
     7681 cggaagagct cggcggacca ggccccctcg tcacccgacg ccccggcgcc gaaggacgaa
     7741 gatcctcagc tctcacccga cgccccggcg ccgggagaaa gttcggacca ataggtcttg
     7801 ggtcgccccc cgggggcgag gcccttctgg agctagtccc ggttcgcctt tgggacgggt
     7861 cggcttgaca ggggggccat tcccccgtag aatatgacgc gctttggtag cgaaccggtg
     7921 gggtggccga gtggctgaag gcgctcgcct gctaagcggg taggggggcc aaaacctccc
     7981 tcgagggttc gaatccctcc tccaccgcca tggatctgaa gcttcgcgat ggatccgcag
     8041 tgacttcact aaagacgaag acgaagccgg aagggccctt ggagccttcc ggcttcgtct
     8101 gttcggatgg gacgatctcc ctctgaaacc ggataaagct ttggagggca gaacgacccg
     8161 actttcgcac aaaaaggccc ttattcctcg ccatcgctag ctttctataa aaaattaggc
     8221 actacgagat gtcctcctcc tccgggagaa agatcgccat cgtagtccac attccgcgag
     8281 aaagccatcg gggagatggg cgctcccggc aaaagaaagc gaaagtcggg ataaagctct
     8341 ggagggcgga acgacaagat ctcccatccc gtagacccca cgggaccccg gatggtacgg
     8401 tgccggggct tcagtgacac ggtgtcggat tgtcagtggt acggtttttg gcacggtcgc
     8461 gacatgcgtt ggggaatgcc atgcgagacg gtgagacggt gcagaacagg tgcaccttgg
     8521 aatcgctggg gttcggggga ggaatagggc cgatggggac gccgcgagat ggtcgagtgg
     8581 cggaggacga gggattcgaa cccccggagc ttgcgctctg ctgatttcaa gtcagccgcc
     8641 atcgaccgct cggccagtcc tccgcagggc ccaagtatag caaaaacccc ttcgagggcg
     8701 caacggggct ggtatcaccc atggccggac ctcgatatgc ctccccggga cgggacggga
     8761 aaaagctccc cccggacgaa gaggaggcgt gtttatgaaa cgataggacc gtgatcatcg
     8821 atgccgagga tcctccgcca aaggagtgaa acggccgagg ccccttgccc aggaggatag
     8881 gtggggcggg accgcgtggt cttttcccca tcgccgtgac gtcgcggcgc gattgttctt
     8941 gtgcctgctg aaaaagtttc tattttctcc ggggagcttt tccccatcgg aaagatatat
     9001 gtgctatgct tgtccccggg gtgatgggtg aaaggagatc cgtcgctttt ctcgggagga
     9061 ggggagggaa ctcttcggcg cgggggcgcg ggacgctgga gacctcctgg cgggccctcc
     9121 gtaagcccct gtggcggaag ggtcttggct tagggcggat gggggatcct tggggcgatg
     9181 aaaagactga acgggacaaa aaaggggcgg gatacgatga ccatggctga gatcatcgaa
     9241 cgttttatct gcattccctc gacggtggcg gtggtaggcg cctcccccaa ggaggatcgg
     9301 tcggtctttt cgatcatgtc ctatctgcac gaggcgggat tccatttgca ccccataaac
     9361 cctggttacg cgggggaatc catccagggc tatacctgcc tggcttcgct ggatgacctc
     9421 ccggagccgg tggacgtggt ggcgcttttt atttccgccg agcaccagga gggcgtggtg
     9481 caaagccttc gccgattgcc ctataagccc atcgtatggt ttcagccggg atcggaaaac
     9541 gcggaccaag aaagggccct cgccgccgcc ggttacccgg tggcggtcaa ttcgtgcatg
     9601 atgatgatcc atcaggtcta ctgccactga gggagcttcg tcgggccagg gaaaggagca
     9661 tgacatggcg ggaaagatag tggcggtctg tgtggcccct gccaaaggca tccccaaaga
     9721 agacgtggga gaagggcttc tggtggagga agaaggtctg gagggggatg cccatcgggg
     9781 tttcgcccat cgccaggtta gcctgctcaa tgcggaagac atcgcccggg cccgggagaa
     9841 gatgccctcg ctcggaccgg gaagctttgc cgagaacctc acggtggagg gcttcgatct
     9901 ggggcagctt aaggtgggag atcggatcac ggtgggagac gccctgctgg aggtctccca
     9961 gatcggcaag gagtgtcaca cccgatgcgc catccaccac gccacggggg attgcatcat
    10021 gccccgccag ggcgtgttct gccgggtacg ccggggggga tgggtgagac gaaacgatct
    10081 ggtggatcgg gcccatggag cggggcccgg ggaggccccg gcatgaagag caaggaagct
    10141 ccgggaaggg ccgccacagt gcgggtgcgc tatgccgaaa cggatcgcat gggcgtggtg
    10201 tatcacagca actatctggt gtggttcgag gtgggacgta cggagctctg ccggagcctg
    10261 gggttcccct accgggcctg ggaggccgaa ggggtctttt tgccctgcgt ggaggcccat
    10321 tgccgataca agcaccccgc ccggtacgac gacgaggtgg ccatcctcac gggggtcaag
    10381 gagctcacgc cccacacggt gaccttcggg taccgggtgg agagggaggg gcgcttgttg
    10441 gccgaggggt ggaccaagca cggcttttgc aatcccgagg ggaagctgtt gcgcgagccc
    10501 cagccctttt tcgggatgtt gggcgacttt ttggcgtttt cgaaggatac ctcaaagagg
    10561 gaggaatgat gggtgagtaa gcgtatggtg ttgctgggca acgaagccat tgcccgggga
    10621 atcgtggagg cggggtgcga ggtggcggtg gcctatcctg ggactccttc gtcggagatc
    10681 ctgccggcgg tggccaagta tgccgacgag ctgggcacca ggacggcggt ggagtggggc
    10741 gtgaacgaga aggtggcctt cgaggtggcg gcggcggcgt cttttgcggg gaagcgttcc
    10801 tgcgccatca tgaagcaggt gggggtcaac gtggcggcgg acgctctcat gagcgtggcc
    10861 tacttcgacc tcgccggggg atttgtgttg gtggcggcgg acgaccccgg cccccatagc
    10921 tctcagacgg agcaggatag ccggagcttc gcccagtttg ccaagattcc ctgcttcgat
    10981 ccctcgtcgg cggtggaggc gaagcacatg gtcttcgacg cctttgatct ctccgagcag
    11041 taccacatcc ccgtcatgct ccgccccgca gggcgggtga accactgccg ggaagacgtg
    11101 gagctcgggg ccgtgcgtcc gggggatcac ccggcgaagt ttgtcaaaga cgtccgccgg
    11161 tgggtctgcc ttccggccca cgtgcgcctc acccaccccc agctcaacgc caaaaacgac
    11221 gccatccgtc aggattttga gagccgattc ggggcgtata actacgaact ggcggcgaaa
    11281 aagcccgcca agctgggcat tgtggcggga ggggtgtctt ttgccatcgt gcaggatctc
    11341 ttggcctcct ggggccggga ggacgtggcg gtgctcaaga tcggcactcc ggtgcccctg
    11401 ccggaaaaaa tggtgatgga tttcatctct aggcatgata acgtcctgat cctggaggaa
    11461 acgtaccccg tcatcgaaaa ccaactcccg gaccgtacca aggtcaaggg gcgccataac
    11521 ggcttcgtgc ctcgggccgg agagctcctc cccgaggtca tcgaagagct ggtcctcagg
    11581 gcactggggg agcccggtgt cccgggggca tctccggagc tcaaggcggc cctggaggag
    11641 cttcagatca aacccaaacc ccccatgctt tgcccggggt gcccccatcg ggcgagcttc
    11701 tttgccatcc gcaaggcggt gccaagcggc atctacccct cggacatcgg gtgctacacc
    11761 ctcggagtga accagcgcat ggtggattcc gtcatctgca tgggggcctc cgtgacccag
    11821 ccctcgggtt tttacctggc ccaccgggtg gatggcaagg agcagcccct cgtttccacc
    11881 atcggcgatt ccaccttctt ccacatgggg atccccggct tggtgaatgc ggtatacaat
    11941 cgccacgcct tcgtgttggc catcctggac aacagcatca ccgccatgac cggagggcag
    12001 cccaacccca acgtgggcac caagctccgc aagggagatg tgggagcccg ggtggacatc
    12061 gaaggcgcgg tgcgggggtg cggcgtgggg cacgtggaag tggtggaagc ctatgacatg
    12121 gcgtcgggag agaaggccgt gaaggccgcc tgggagtacg ccaaggcgca tcagtccccc
    12181 gccgtggtca tcttccgcca tccctgtatg cttctgcgtt gcgagcagga aaagatccgg
    12241 gtgcgagtgg acgaagagcg ctgtgtgggg tgcaagttct gcatcaactt cttcaactgc
    12301 cccgccctgg tctttgacga agggaaaaac aaggccgtgg tggatgaccg tttctgcgtg
    12361 cagtgcggca cctgcatccc cgtgtgtccc cacggagcca ttgtccgcgt ttcggaggag
    12421 gcgtaaccat gcagtacatc gtcgtgggcc ttgggggcca ggggatcttg ttcagcagcc
    12481 gagttttggg acacatcgcc gttgccaagg gggagcacgt catgggcagc gaggtccatg
    12541 gcatggccca gcgcgggggc tccgtggtga gccacttcaa ggtgggcccc tacgccagtc
    12601 cgctggtgcg cgccggaaac gcggatattt tgttggcctt cgatcagaac gagggggtgc
    12661 gaaacctgca tttcctgaaa cccggaggga aggcggtcat caacgtacac aagcccgaga
    12721 gcttggagaa cgcccatctg caggccttca tcaagtcccg taatatccat attgccctca
    12781 tcccaggata tgatatgctg cgacagcaca tggggggcaa cttcctattc ttgaacgttc
    12841 tcatcctggg agctctggcg gggacgggta tggcggacgt gtccatggaa gagatccgcc
    12901 aggccgtggc ggagctggct ccggccaagg cccgggacgc gaatctggcg gtgctggagc
    12961 tgggatacaa gcatttcgcc tcctaaacgg ggcgagggcc cgattctaga gaacgaccaa
    13021 gaaaaggggg ggctttccct aagagggggc tccccccctt ttgcgcccag ggggaggtct
    13081 cgccgggtga catacgagag aggatgggta aatatggcgg caaaatcatg gtggcgggag
    13141 accatcgaga cgattctttg ggccctggcc ctggccctga tccttcggac cttcgtggtg
    13201 caggcgttct ggattcccag tgggtccatg attcccacgc tggaggtgag cgaccgggtg
    13261 ctggtggcca agttttggta tacgttcttc gagccccgcc gaggagaggt ggtggtgttc
    13321 aagtaccccg tggaccccac ccgggacttc gtcaagcgca tcgtggggct cccgggggat
    13381 gtggtctacc ttaaagacgg cgtggtctac gtgaacgacc aggccctgga cgaggcttat
    13441 gttaaaaatc acgattcttt taacatgccc tccctgacgg tacccccggg gcactacttt
    13501 gtcctgggag ataaccgccc taactcccag gatgggcgct tctggccctc ggggagccag
    13561 tttgtcccca agagcttcat cagggggccc gcctttttcc gctactggcc cctggatcgt
    13621 ctggggctcc cctactaggt catggcgggg cgcacggtat ggtatccggg gcacatggcc
    13681 aaggggaagc gagccctgga ggagctggct tccaagttgg atgtgctgct ggaggtgcgg
    13741 gacgcccgag cgcctcgcct caccgcctct tttcaggccc aggagctccg acggctgcgc
    13801 cccgtctggg tgatcctcgc caagcgcgat ctggcggaag agggagtgac ggcgtcttgg
    13861 ctccgctggc tggaacggga cggaggtcgg gcctgggcga tggatctgcg aaagggccgc
    13921 ctggactacc tccggcgggc cctgcgggag atggcccccg ggcatcgaga ggctagggtg
    13981 gccgtggtgg gagtgcccaa cgtggggaaa tccctgcttt tgaaccagct ccttggaggg
    14041 gctcaccggg ctccggtggg ggggatcccg gggatcacga agggcgtgtc ctggtaccgg
    14101 gtggagggga atctgctggt tgtggattct ccgggcattc tggatcccaa agcggaggcg
    14161 gggatccagc ggtccttggc ctggctgggg agttccaagg gagaagtcat cggcggagcg
    14221 gagtccctgg cggtggacct gctgcggtat ctgcgggatc acggcctttg gggcgtggtg
    14281 gagcgcacct ggggggtgtg cccctcggag atccccgagg gggcggagga gccggacgac
    14341 ctggacctcc tgggacgccg cctcggctgc ctgctccggg ggaaccgggt ggatcgggag
    14401 ctggcgggac gccgcctttt ggatgccttt tcccagggga aggtgggccc cctgtccctg
    14461 gaacggccgg gagaaggggg gggcggggcg tgatcgtggc gggcacggat gaggcggggc
    14521 ggggtccctt ggcggggccc gtggtggctg cggcggcggt gctggagccc tttcagatgc
    14581 gagagttgat gggtttggga ttgcgggatt ccaagcgcct ttcccctcgg cgccgggata
    14641 tcatcttcgc ttccctgggt cttttggggg tggtctggcg cgcccaggcg gcgtctccgg
    14701 cgcgaattga ccgagacaac atcctccagg cctcgctatg ggccatggga cgggccgttt
    14761 tgtccctccc cgagcgcccc cacctggtgg tggtggacgg cactgtggag atccctgggt
    14821 t
//
LOCUS       DAOOER010000045        14218 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_4852_length_14218_cov_9.191979, whole genome shotgun sequence.
ACCESSION   DAOOER010000045 DAOOER010000000
VERSION     DAOOER010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 14218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14218
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4852_length_14218_cov_9.191979"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PL162_06755"
     CDS             complement(<1..818)
                     /locus_tag="PL162_06755"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQF91752.1"
                     /translation="MESRIRVRGIDLAYEERGEGRPMLLLHGFKPDRRLMQGAFEPVF
                     EKRSGWRRIYLDLPGMGASGAGPAAATSDGMLDTVLEFIDAVLPHGTFAVAGQSYGGA
                     LARGVVKDRGARVEGICLLCPMTVEDFTKRHLPPRQVVAPDCSLRERLSPQEWEEFDA
                     IAVAESPRIWERTRDEIFVGVHAAQDQFLDALRAHNMAFSRDIDALERPFEGPGLFVM
                     GRQDHRTGYEDVWPLLANYPRATFAVLDRAGHHLHIEQETLFNALVHEWLHRME"
     gene            complement(821..1384)
                     /locus_tag="PL162_06760"
     CDS             complement(821..1384)
                     /locus_tag="PL162_06760"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HQF91753.1"
                     /translation="MYVLALMGSPHDQGNTATLTQDALDAAAARGAETRMVRLNELTI
                     RGCQGCYGCQRETPCVQQDDMTTLYEEINRADAVLIASPVYMGYPFTAQTKIFIDRLF
                     PYIQVLSDRKSRMKPGKRLGLIITQNQPASDFYRAKVEELETMLHFLGFGEGRAMLAA
                     DLGGPTDAARQDYREEARHLGEFLAGI"
     gene            complement(1387..1875)
                     /locus_tag="PL162_06765"
     CDS             complement(1387..1875)
                     /locus_tag="PL162_06765"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQF91754.1"
                     /translation="METDKNSPGAAHPRPALYLAELVRARMHSYLEKKFAEAGVTDLS
                     VSHGSILFWLYRSGGAENMLRIAEKIQRDKSTVTYLTRKLVEAGYARRRQCTVDRRSV
                     FIELTEKGWRFQELFERVNKELTETAYKNFSDEEAHTLRQLLEKMASNLDENRPVYDK
                     EE"
     gene            2042..2947
                     /locus_tag="PL162_06770"
     CDS             2042..2947
                     /locus_tag="PL162_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQF91755.1"
                     /translation="MAEMLDRLSLTVVVDDAAGQDSALRAQHGLSVWMEGVAGEQTLR
                     LLLDVGQSFDTLRHNMSLLGLAPGSLDALVLSHGHYDHTGGMASLVRATDKALPVVAH
                     PDLFRPIVFLEPGPRSIGVRPEDQRPAVQAAGGVLLLSTDPVPLGPGMTTTGVVPRSS
                     GFEESLLAAYTVAEGHLRTDPLEDDLAVVARIRGRGLVVVTGCSHAGIVNVVRRSMEL
                     FPGEALDGVVGGFHLGSASAERLTRTLDALEELAPRWIAPGHCTGFEAQRAFKERFGA
                     AFVRPYAGLVLTVAPDEDLAPRMGA"
     gene            3153..>4619
                     /locus_tag="PL162_06775"
     CDS             3153..>4619
                     /locus_tag="PL162_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HQF91756.1"
                     /translation="MATGKLTDDPVLLEEFFAAAKGGDKKDGGQGIPEEEGGTEEALE
                     GAFCPWIYAPSGEHGVFRNASPFALLCIGAVLEKDGTLRSTGLPWIPRTLLQPLNDDG
                     VTVGSVEDSDAALTALAAQPSKEDSWQETARVCREYLEAVTGSSAPPDLPGYTRVPGG
                     AFVAGAPIGVSRHVQALYDALLDKKPSAPLLERLVQGQAPSSPLLTADEEIRVSRKHW
                     GTMSGKFPLSSSQRRAMHHFLGMHDGEVLAVNGPPGTGKTTMLQSIVAQLWVDAAYNR
                     RECPLVVASSTNNQAVTNIIDSFGKVADASGDALYTRWVPDVKSFGLQMARKERWKDA
                     FHAHDDRSLQDGFFATIENPAWRERATTFFLEKCQGRYGVVTDLNGAVEALSAELKDV
                     VERIDGVIGTASALAGEDHKKDLFSLYEEALQRRQQEQTVLKTNLAILRLRKSAVKGW
                     AVEWERFVRDESVFLAIFSFLPPIRKKREAATRAFLLAR"
     assembly_gap    4620..4719
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4720..6079
                     /locus_tag="PL162_06780"
     CDS             <4720..6079
                     /locus_tag="PL162_06780"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQF91757.1"
                     /translation="LEAHLKFFGIDAGGGALSLRGLLEEIDQRGRSRAFYLATHYWEA
                     QYLLELGEKLCEEAKNASSGSTAKYDSKAPAKRIRMYRRFAKLTPCQVITFYMLPHYY
                     CGYLGHPIHLFGEIDLLIVDEAGQVSPEIGVPSFSLAKRALVVGDTFQIEPVWSVMRR
                     TDEANAVKDGVVLSPEEYDGLSRKNLSPEAYEKALVEKGCLAGSYEEGQCRGFLAASG
                     NLMRLAQRACRHTDYPDLAPGLMLTEHRRCVPEIIAYCNDLVYRGKLEPRREESPEER
                     RRRPDFLPPLGEIDVKGSEERAGGSRRNAKEAEATAQWVKEHAEGMKIRYENKPLEEI
                     LGIITPFAAQKKCILDALANSLGPEHGITVGTVHALQGAERSVVLFSPTYDQNHTGDM
                     FFDRGSNMMNVAVSRAKDSFLVIGATALFQEENLSPSGLLARHIRAIPAPQDHGSRNE
                     KP"
     gene            complement(6448..7026)
                     /locus_tag="PL162_06785"
     CDS             complement(6448..7026)
                     /locus_tag="PL162_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HQF91758.1"
                     /translation="MDEARLFHRMLDVIEDDILPLTRRTVPEGHKIFGAAVLRAEDLS
                     LVRAGTNHEAWCPLWHGEIYTIKEFFEDKTHPRPEECLFLSTHEPCSMCLSALAWSGF
                     PRVYFFFGYEQTEGDFHIPHDLRMLDEIFHCRKPARKSSFLEMTPLFDLMPGLPDPDA
                     ARARCERIRRAYVDVSAVYQAIKDHSVEIARK"
     gene            8320..9936
                     /locus_tag="PL162_06790"
     CDS             8320..9936
                     /locus_tag="PL162_06790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SYNERG-CTERM sorting domain-containing protein"
                     /protein_id="HQF91759.1"
                     /translation="MYRPDVTADLHEMGFGVTAERDASRRTLGYKDYQLEEVQTLVAP
                     LKNVPTAVREMATNLFEANVDRDLRARHMRSHWYTMSDGAYISADVWDGTKLVSGEVY
                     QISTLQEGNPDEGITDPSFSLKPAISILFESRSPKVRVNYMSRTYAHSLAVESVLRTA
                     ATNADALRATMRAARQEMIDLGKTVDPNDQVVLWSAHERVPGQQRETLVFNADRTRLE
                     TRFIPFTALRVKNLTTLKSVVRPRAYIIPATVPDLDKVAVRLACTGARIERLAQETPL
                     EVEAYTITGTGRPADQAPRGSGFSGLSLIVTDVSSTVKTVTFPKDSLVVYMDQTAGTH
                     AALALEPMGNRSYANYWITHVNDRSGGEIGFLPAALNQEFPAYRYMKNDRLNSYAVIV
                     DAPLVDGTMITDVMPLTREQMEDYEREVGGTLSYVSHFRVMDATDSFTVTLPYSYGET
                     SLVDSVWYLYDWAAGEFEALQERNVSQGVTDVQVPAKFISATKEVLLAARSITLPSSG
                     GGGGCDATGTAGMALTLLAPLTLALFWRKR"
     gene            10619..11842
                     /locus_tag="PL162_06795"
     CDS             10619..11842
                     /locus_tag="PL162_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91760.1"
                     /translation="MKTHHQDEPLVRSAPPTGIAAPVDFSLETVILMIGGEALVLAGG
                     TLLGAASGAFPFAESGLRGLLIFIFALQALTLGKTPFGATRRSSGMIGLWMAVASMGI
                     VAGMIPDVLDPLPRGLLGLCFAGGGGAQLVSLVLDGERRALWKREGGPLRPLTPLCAT
                     VYALSIPVGLALGGVFSPPGAGTAVLLSLLGGALLLLAAVLEAVYRRYPEARRAVETS
                     EPLSFSHTMILVTGVFMVLLGILLVPVVLGALPFSPSAQLGLLLLIMALRTMAAGETP
                     LGTFPRTRPVVAAGLLFSMLGTFSCLVPEVLVAPLVVLVGGLNVAGGALPLLRRGPSP
                     EHPLARRLAKTQDILNVLAILFGLSMLFPGIISGLVTGVLLAANGGVLLYLLHLLRQL
                     EALRASLESSEGRAP"
     gene            11946..12755
                     /locus_tag="PL162_06800"
     CDS             11946..12755
                     /locus_tag="PL162_06800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQF91761.1"
                     /translation="MRAYIENKVAVITGGASGIGLALGEEMLSLGARRVVLADLNEEK
                     LRKESERLDATYPGKVLGVPTDVTNRERVFSMIEKASEFGEGRIDLLLNNAGLGLAKA
                     FDDTTEEDWTFAFNVNFYSALYGIRAVLPIMRSQEGGGHIANTASGIVFSPMPLQSMY
                     SATKAALMALTSSLRSELWDDHIRLSTVIPGTVATPIWDKAGGSPKGAITPQESAQGI
                     IKGLAANERIIIITEEDKSGARNHFLPEYSEGVDAYFLDVARRRKRGEIVI"
     gene            13142..13819
                     /gene="prxU"
                     /locus_tag="PL162_06805"
     CDS             13142..13819
                     /gene="prxU"
                     /locus_tag="PL162_06805"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048998.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:13337..13339,aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HQF91762.1"
                     /translation="MESPEPKDREIPKKEASGMTIMVGRKAPDFTAPTYRQGKFGTVS
                     LGEYLGKWVVVCFYPGDFTFVUATEVSAVAENYPKFQALGAEVISISVDSPFVHKVWN
                     DVELSRMVEGGVPFSMASDSGGAIGRAYGVYDDVAGVDVRGRFIIDPDGVIQGMEVLT
                     PPVGRNVEETLRQLQAFQHVRATKGSEVTPSGWTPGGKVLRPGIDLVAKVWQEWNPRD
                     VYGEKKG"
BASE COUNT         2637 a         4211 c         4485 g         2785 t
ORIGIN      
        1 tcctccatgc ggtggagcca ctcgtggaca agggcgttga aaagggtctc ttgttcgatg
       61 tgcagatggt gccccgcccg gtcgagcacc gcgaaggtgg cgcgggggta gtttgccagc
      121 aacggccaca cgtcttcgta gcccgtccgg tggtcctggc gtcccatgac gaagagcccg
      181 ggcccctcga aggggcgttc cagggcgtcg atgtcccggg aaaaggccat gttgtgggcc
      241 ctgagggcat cgagaaattg gtcctgggcg gcgtggactc ccacaaaaat ctcatccctc
      301 gtccgctccc agatgcgggg cgattccgcc acggcgatgg cgtcaaactc ttcccactcc
      361 tgcggtgaca gacgctcccg caggctacaa tccggagcga cgacctgccg ggggggcaga
      421 tgccgttttg tgaaatcttc cacggtcatg gggcagagga ggcagattcc ctccacccgg
      481 gctccccggt ccttcaccac gccccgggcc agggctccgc cgtaggattg ccccgccacg
      541 gcgaaggtgc cgtggggaag cacggcgtcg atgaactcca aaaccgtatc caacatgccg
      601 tcgctggtgg cggcggcggg ccccgccccc gaagccccca tgcccggcag gtcgagatag
      661 atgcgccgcc atccggagcg tttttcaaac accggctcaa aggccccctg catcaggcgc
      721 cggtcgggct tgaagccgtg cagcagcagc atggggcgcc cttctccccg ctcttcgtag
      781 gcgaggtcga tgccccgcac ccggatccgg gactccatgg ctagatcccc gcgaggaatt
      841 cgcccagatg tcgggcttct tcccggtagt cctgccgggc ggcgtcggtg ggcccgccga
      901 ggtccgccgc cagcatcgcc ctcccctcgc cgaagcccag gaaatgcaac atggtttcca
      961 gctcttccac cttggcccgg taaaaatccg atgcgggttg gttctgggtg atgatgagtc
     1021 ccaggcgctt tcccggcttc atcctgcttt ttctgtcgga aagcacctgg atgtacggaa
     1081 acaggcggtc gatgaagatc ttggtctggg ccgtgaaggg atatcccatg tagaccggcg
     1141 aggcgatgag cacggcgtcg gcccggttaa tttcctcgta cagggtcgtc atgtcgtcct
     1201 gctggacgca gggcgtttcg cgctggcagc cgtagcaccc ctgacatccc cggatcgtga
     1261 gctcgttgag ccgcaccatc ctggtttccg ccccccgcgc tgcggcggcg tcgagggcat
     1321 cctgggtcag cgtggcggtg tttccctggt cgtgggggct tcccatgaga gcgagaacgt
     1381 acatgattat tcctccttat cgtatacggg acggttttcg tcgagattcg aggccatttt
     1441 ctccagcaac tgccgcagcg tgtgggcctc ttcgtcggaa aagttcttgt aggccgtttc
     1501 ggtcaattct ttgttcactc gctcgaagag ttcctgaaat cgccacccct tttcggtgag
     1561 ctcgatgaag accgatcgcc ggtccaccgt gcactgcctg cgccgggcgt accccgcctc
     1621 caccagcttg cgcgtgagat aggtcaccgt cgatttgtct cgctggatct tttccgcgat
     1681 gcggagcatg ttttccgctc cgccgctcct gtagagccag aaaagaatgg agccgtggga
     1741 gacggagaga tcggtgaccc ccgcttcggc aaattttttc tccagatagc tgtgcatcct
     1801 ggcgcgcacc aactccgcca gatagagcgc gggccgaggg tgggccgccc ccggagagtt
     1861 tttgtcggtt tccatgtcac cccccccgaa tttttgtttg atctcaaact atgtggtgat
     1921 gatagtttga gatcaaacaa aagtcaaggg gcacatcgaa aaaaaaggag cccccttggc
     1981 aagaggaacg ccctgtgctt tcatcgaagg cgacgccgga ggcgcgaagg aggacgagat
     2041 catggccgaa atgttggatc gcctttctct caccgtggtg gtggacgatg cggcggggca
     2101 ggacagcgcc ctgcgggccc agcacggcct ttccgtgtgg atggaggggg tggcggggga
     2161 gcaaaccctg cgccttctcc tggacgtggg gcagagcttc gacaccttgc ggcacaacat
     2221 gtcgttgctc gggttggcgc cggggagtct ggacgccctg gtgctctccc atgggcacta
     2281 cgaccacacg gggggcatgg cctccctggt gcgggccacc gacaaggccc tccccgtggt
     2341 ggcccatccg gacctcttcc gccccatcgt ttttctggag ccgggccccc ggtccatcgg
     2401 cgtgcggccg gaagatcagc gccccgccgt gcaggcggcg gggggggtac tgctcctgtc
     2461 gacggacccc gtgcccctgg ggccggggat gaccaccacg ggggtcgtgc cccggagttc
     2521 cggctttgaa gagtcccttc tggccgccta tacggtggcg gaggggcatc tccggacgga
     2581 tcccctggag gacgatctgg cggtggtggc ccgaattcgg ggaagggggc tggtggtggt
     2641 gacggggtgc agccacgcgg ggattgtgaa cgtggtgcgc cggagcatgg agctctttcc
     2701 cggggaagcg ctggatggag tggtgggggg atttcatctg ggctccgcct cggcggaacg
     2761 cctcacccgc acgctggacg ccctggagga gctcgctccc cggtggatcg ccccggggca
     2821 ctgcacgggg ttcgaggccc agcgggcctt caaagagcgc ttcggcgccg ccttcgtgcg
     2881 cccctacgcg gggttggtgc tcaccgtggc tccggacgag gacctcgccc cccggatggg
     2941 ggcctgaggc tccgcagaat tcaaaaaggt gttccggcga tgccaagacg cccccggggg
     3001 ggcgcaggtc acggggggga atggcccatg gaggaaacga cgcggagggt tctttcgtat
     3061 taccgccaca cgttgggcga cgcggcgaag caaaaccccg atgtaacggg aaaggacggg
     3121 gggcgaagct cctccggggg aaggaagacg ccttggccac ggggaagctg acggacgacc
     3181 cggtccttct tgaggaattt ttcgccgccg ccaagggggg cgacaagaag gacgggggcc
     3241 aggggatccc cgaagaggag ggcggcacgg aagaagccct ggagggggcc ttttgtccct
     3301 ggatctacgc cccctctgga gagcacgggg ttttccggaa cgcttctccc tttgccctgc
     3361 tctgcatcgg ggcggtgctg gaaaaagacg gcaccctgcg atccacgggt cttccctgga
     3421 ttccccggac ccttcttcag cccctcaacg acgacggcgt caccgtgggc tccgtcgaag
     3481 actccgacgc cgctctcacc gcccttgccg cccagccttc gaaagaggac tcgtggcaag
     3541 agacggcccg ggtgtgccgg gagtaccttg aagccgtgac ggggtcttcc gcccctccgg
     3601 acctcccggg atatacccgc gtcccgggcg gggccttcgt ggcgggggcc cccatcggag
     3661 tgtcccgcca tgtgcaggcc ctctacgacg cccttctcga caaaaagccc tcggcgccgt
     3721 tgctggagcg tctcgtccag ggccaagccc catcctcccc tttgctgacg gcggacgaag
     3781 agatccgggt ttctcgcaaa cattggggca ccatgtcggg gaaattcccc ctctcctcct
     3841 cccaacgccg ggcgatgcac cactttctcg gcatgcacga cggagaagtg ctggcggtga
     3901 acggccctcc gggaacgggc aaaaccacca tgctccagag catcgtggcc cagctctggg
     3961 tggacgccgc ctacaaccgc agggaatgcc cccttgtggt ggcctcgtcc accaacaacc
     4021 aggccgtgac gaacatcatc gacagcttcg ggaaagtggc cgatgcctcg ggagatgcgc
     4081 tgtacacccg gtgggtcccc gacgtgaaga gcttcgggct ccagatggcc cgaaaggagc
     4141 gatggaaaga tgccttccac gcccatgacg accgctcttt gcaggatggt ttttttgcaa
     4201 cgatcgaaaa tccggcgtgg cgggaacgcg cgacaacctt ctttctggaa aagtgccaag
     4261 ggcgctacgg ggtggtgacg gatctgaacg gggcggtgga agccctttcc gccgagctca
     4321 aggacgttgt cgagaggatc gacggggtca tcggcacggc ctcggcgctg gcgggcgagg
     4381 accacaagaa agatctcttc tccctgtatg aagaagccct tcagaggcgg cagcaggagc
     4441 aaacggttct gaaaacgaat cttgcaatcc tgcgcttaag gaaatcggcc gtgaaaggat
     4501 gggcggtgga gtgggagcgc ttcgtccgcg acgaatccgt ttttctggcg atcttttcct
     4561 ttttgccccc gatacgcaaa aagcgcgagg ccgccacgcg ggcctttctc ctcgcccggn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc ctggaggccc atctgaagtt
     4741 ttttggcatc gatgccgggg gcggggcgct ctccctccgg gggcttctgg aagagatcga
     4801 ccagaggggg cgctcccggg cgttttatct ggcgacccac tactgggagg cccagtatct
     4861 tctcgaactg ggggagaaac tgtgcgagga ggccaaaaac gcttcatcgg ggtcaacggc
     4921 gaagtacgac agcaaggctc cggccaaaag aattcggatg taccgccggt ttgcgaagct
     4981 gaccccgtgt caggtgatca cgttttacat gttgccccat tactactgcg gatatctggg
     5041 ccaccccatc cacctcttcg gagagatcga cctgctcatc gtggacgagg cgggacaggt
     5101 gagccccgaa atcggcgtgc cgtccttctc gctggccaag cgggccctgg tggtggggga
     5161 cacgttccag atcgaacccg tgtggtccgt gatgcgccgc accgacgaag ccaacgccgt
     5221 aaaagatggg gtggttcttt cgccggagga gtacgacggg ctgtctcgga agaacctttc
     5281 gccggaggcg tacgagaagg cccttgttga aaagggatgt ctggcggggt cctacgaaga
     5341 aggccaatgc cggggatttc tggcggcgtc gggaaacctc atgcgcctgg cccagcgggc
     5401 ctgccgccac accgactatc cagacctcgc ccccggactc atgttgacgg agcaccgccg
     5461 gtgtgtgccg gagatcatcg cgtactgcaa cgacctggtc tatcggggca aactggaacc
     5521 ccgccgagaa gagagccccg aggagcggcg tcgccgcccc gactttcttc cgccgttggg
     5581 ggagatcgac gtgaagggct cggaggaacg cgccggaggg agccgcagga acgcgaagga
     5641 agcggaagcc acggcgcagt gggtgaaaga gcacgccgaa ggcatgaaga tccggtacga
     5701 aaacaagccc ctggaggaga tcctgggcat catcaccccc ttcgccgcgc aaaaaaaatg
     5761 cattctggac gcgctggcca actccctggg gccggagcac ggcatcaccg tgggcaccgt
     5821 ccacgccctc cagggggcgg aacgctccgt ggtgctcttt tcccccacct acgaccagaa
     5881 ccacacgggg gacatgtttt tcgacagggg gtcgaacatg atgaacgtgg cggtctcccg
     5941 ggcgaaggat tcctttctgg tgatcggcgc caccgccctc ttccaggagg aaaacctttc
     6001 gccctcgggg ctcctggcac gacacatacg cgccatcccc gcaccgcagg accacggctc
     6061 tcggaacgaa aaaccttgaa gcgggagcac ggtcgcgggg aactccgccg caggttaaac
     6121 ccgtaggggc gagcgtattt ttcgccccta cacggcctcc ccgcagacca tccccccatt
     6181 ccctcaatcg acgtcagcag cgccgaaagc acgtggccgg ggagcgcggg gctgaaaaca
     6241 cgtggccggg agcgcgcacc ttccagccgg cattggtcca ttttttttat ggtcggggtt
     6301 cgcatggcga ggagcgcgca tttttcagcc ggcatcagtt tatttttcga gaaaattttt
     6361 cattaaaact cgcatctttg ttccaggggg aaaagggtca tttctccaca ccggtagggc
     6421 catggggcgc ggattttgcc ggtgccatta tttcctcgcg atctcgacgc tgtggtcctt
     6481 gatggcctga tagaccgccg acacgtcgac gtacgcgcgt cggatccgct cgcatcgggc
     6541 gcgggccgcg tcggggtccg gtaatcccgg catgaggtcg aaaaggggcg tcatctcgag
     6601 gaacgagctc tttcgggcgg gctttcggca gtggaagatt tcgtcgagca tccggaggtc
     6661 gtgggggatg tggaaatctc cctcagtttg ttcatagccg aagaagaagt acaccctcgg
     6721 aaagccgctc cacgccaggg cggagagaca catggagcag ggttcgtgcg tcgagaggaa
     6781 gaggcactcc tccgggcggg gatgggtctt gtcctcgaag aattccttga tcgtgtagat
     6841 ttcgccgtgc cagagcgggc accacgcctc gtggtttgtc cccgcgcgca ccagagagag
     6901 gtcttcggcg cgcagcaccg ccgcgccgaa gatcttgtgt ccctccggca ccgtccgccg
     6961 cgtgagcggc aaaatgtcgt cttcgatgac gtcaagcatg cggtggaaaa gcctcgcctc
     7021 gtccatgccc attcctccct tcggctcgtg cccctggagg gcccgttgtc atggtacacc
     7081 accatcttcc ggacgtccat gctccgcagg gcggcgagat gcctgggccc gggggttggg
     7141 actttttgcc ggaagccact ggcagagcat cccatagcga tggaggatcc catgagaaga
     7201 atatgtcgat cggagctcgc gttggcggat ccagcccacg gtctcaatcg tcagagaatc
     7261 tctggggcga gcatttttgg tgctttggaa aggaagattg ggccattctg ccccggaaaa
     7321 gacagccaaa tccccggaca tacttgacat ttgtcatagg cctcccctag gattgctaca
     7381 cgtaggcgtg cagatgggcg catgggatga acgaggaaaa gatgacgctt tgctacggca
     7441 ccgaggacgt ttgcattcag gcgtggttcc ttgtgccctg cagagagcac aaagcctttc
     7501 cgctggcgtc tgcggggagt tctgttggcc ataaaggagg atggccgtag gtagatggaa
     7561 cgcgagaagt atcatgtcaa atgaaaggag ttttgtgata tgaggaacaa aaaacgcgtg
     7621 gctgtgtcgg ttttttggtc gctccttctg ttggtgcttt ccgcttcctg tctatgggcc
     7681 ggggtggttc cctacgggga ctataacatt gacagagacc cggtacgagc caagcagctc
     7741 tatcccgata tggtgaaaaa ttggtatgac aacggtgaag ctatggaggg cgtgactctg
     7801 agtactccgg cctttgtggg ggaccatggc tccgacatcc agggagaggt gggctggacc
     7861 acatctgaag aggagatgct cgattttctt caaagccttc ccacgagccg aatgaaaatg
     7921 aaaatagttt ctcaggttcc tacccgcaac gccagtggag atattgagcg catgttcaaa
     7981 attcctcttc tagttttcag caagccctct ctcttcaatg cggccgacct ccacgccctg
     8041 ggcaagccca ttgtgtggct agagggaggc attcatggca atgagacttc cgcatcggaa
     8101 gccatgctgg tggtcgccca gaagcttgct agcgggcaat gggatgacat actggataag
     8161 atctctgttg ttattttgcc tcgttattgt gttgacgggg catttaagta tcagcgaggt
     8221 accgatgcta ttcggccggt ccgaacaaat atggaccaga accgagataa cacctctttc
     8281 gaatctccca taacgcgcat tgttcataga ttggtgaata tgtatcgccc tgacgtcacc
     8341 gcagatctgc acgagatggg attcggcgtc acagcagagc gtgatgcctc cagaaggact
     8401 ttgggataca aggattatca gctggaagaa gttcagactc tggtggcccc tctgaagaac
     8461 gtccccacag cggtacgaga gatggccacc aatctctttg aagccaacgt ggacagagac
     8521 cttcgggctc gccacatgcg atctcattgg tacaccatga gcgatggcgc ctatatctcc
     8581 gccgacgtgt gggatggtac caagctagtt tctggggagg tataccaaat ttccacgctc
     8641 caggaaggga accccgacga aggaatcacg gatccttcat tctccctcaa gcctgccatt
     8701 agtattctct ttgagagccg atctcctaaa gtgcgagtca attacatgag tcgtacttat
     8761 gcccatagcc tggcggtgga gtcggtgctg aggactgcgg cgactaacgc cgacgctctt
     8821 agagctacga tgagagcagc tcggcaggag atgattgacc tgggtaagac cgtggatccc
     8881 aacgatcagg tcgtgctgtg gtctgcccac gagagagtcc cggggcagca gcgggagacg
     8941 ttggtcttta acgccgatcg taccaggttg gaaacgcgtt ttatcccctt tacggctctg
     9001 cgagtgaaga acctcaccac gctgaaatcc gtggtacgcc ctcgagccta tatcatcccg
     9061 gcgaccgtcc ccgatcttga taaagtagct gtccgcttgg cgtgcaccgg cgctcgaatc
     9121 gagcgtttgg cccaagaaac tcccttggaa gtggaagcct atactatcac cgggacgggg
     9181 cgccccgcag atcaggctcc gcgaggcagc ggcttcagcg gcttgtccct catcgtcacc
     9241 gacgtgtcca gcactgtgaa gaccgtgaca ttccccaagg attctttggt ggtgtacatg
     9301 gatcagacgg cggggactca tgcggctctc gcgttggaac ccatggggaa tcgaagctat
     9361 gctaattact ggattactca tgtcaacgac aggagcggtg gcgagatcgg ctttcttccc
     9421 gctgccctga accaagagtt tcctgcctac cgatacatga aaaacgaccg gctaaactcc
     9481 tacgcggtga ttgtggatgc tcctctggtg gatggcacca tgatcaccga tgtcatgccg
     9541 ctcaccaggg aacagatgga agactacgaa agagaggtcg ggggaaccct aagctacgtt
     9601 agccatttcc gcgttatgga cgcgaccgat agttttaccg ttactctacc ctattcgtac
     9661 ggcgagacct cgctggtgga cagcgtgtgg tacctctacg actgggccgc cggggaattt
     9721 gaagccctcc aagagcggaa cgtttctcaa ggggtgacgg acgtacaggt tccagcgaag
     9781 ttcatttctg ctacaaaaga agtgttgttg gcagcccgca gtatcacttt gccctcctct
     9841 ggtgggggcg gaggttgcga tgccacagga acggcaggta tggcgctaac cctcctggct
     9901 cctctgactc tggcgctgtt ctggcgcaaa agataagacc gggatatttc cggaatgagt
     9961 taatctgccc cccccttcgc tctatttgag cagggggggc tttgtcgcac tttaccggca
    10021 gcgagagcta cgggggcaca ttgccatccg cgcaatttat aaaaattccc ccgtggtggc
    10081 gatatgatgg ttatgtgata gggtatgggg aagatccttc tgtcttcggg ataagggccc
    10141 catggaagcg cgtcgccaag gcgacccatg tcaagcgaca ctagccccgg ctcattggag
    10201 tggggtcgaa agcttgcctt cgccagcccg gacgccaagg aatccctaag atattccgag
    10261 aagacggggg gaagcctgtg gccccgacgg gagatcatcg ccacttcact gctatctgcc
    10321 agcgatggag ctgggcgttg tcaagcgaca ccaaaaactg ccccctttgc gacatgagga
    10381 attgtcccac cggccattat ccggatgagg gtcctggcgc cacctccgca acctcccagt
    10441 tcaaagccag cgctcccgga tcgcacgccc gaggggaaag cccgggtata ttacgaagga
    10501 tatactacga aagacggacg cgtccttggt actcccacgt ggtaggatag gaacaaggtg
    10561 gatctttgcc gggagcgttg gcttccagcc ggctctttcg tcctgaggag ggtgctccat
    10621 gaaaacacat caccaagacg aaccgttggt ccgcagtgct ccccccaccg gcatcgcggc
    10681 ccccgtggac ttttctctgg aaaccgtcat cctgatgatc ggaggcgagg ccctcgtcct
    10741 cgccgggggc acgctcctgg gcgccgcctc cggagccttt cccttcgccg aaagcggcct
    10801 ccgaggcctg ctcatcttta tctttgccct ccaggccctc accttgggta aaaccccctt
    10861 cggcgcgacc cgtcgctctt cgggaatgat tggcctgtgg atggccgtgg cgtcgatggg
    10921 catcgtggcg ggcatgatcc ccgatgtgct ggatcctttg ccccgggggc ttttgggcct
    10981 ctgcttcgcc gggggaggag gggcgcagct cgtttccctg gtccttgacg gcgagcggcg
    11041 ggccctatgg aagagagaag ggggccccct gcgccccttg acgcccctgt gcgccaccgt
    11101 ttatgccctg tcgatcccgg tggggctcgc cctgggaggg gtcttctccc ctcctggggc
    11161 ggggacggcg gtgcttttat ccctgctggg aggggctttg cttctcctgg ctgccgtatt
    11221 ggaggcggtc taccgccgtt atcccgaggc gaggcgcgcc gtggaaactt cggagcccct
    11281 gtcttttagc cataccatga tcctcgtcac gggggtcttc atggtgcttc tggggatcct
    11341 tctggtgccc gtggtcctgg gggcgcttcc cttttccccc agcgcccagc tcggcctctt
    11401 gctcctcatc atggccctcc gaaccatggc cgccggggaa acccccctgg ggacctttcc
    11461 ccggacccgt cccgtggtgg cggcggggct actcttttcc atgctgggga ctttttcctg
    11521 cctggtgccg gaggtcctcg tggcgcccct tgtcgtgctg gtggggggcc tcaacgtggc
    11581 cgggggagcc ctccccctcc tccggcgcgg tccttcgccg gaacatccct tggcaaggcg
    11641 cctcgcgaag actcaggaca tccttaacgt attggccatc ctctttggct tgtccatgct
    11701 tttccccggg atcatctcgg gcctggtgac gggagtgctc ctcgccgcca atgggggggt
    11761 gctcctgtac cttttgcacc tccttcgtca gctggaggcc ctgcgagcct ccctggaatc
    11821 ttcggagggc cgggcgccgt gatgggcgcc cttagagatc tatgagagta gtcggaagcg
    11881 gttgcccctc gggctttggc aggaagaccg aaggaagccg ggggaccagg aaaggagagg
    11941 atgacatgag agcgtacata gaaaacaaag tggcggtgat caccggaggg gcgtcgggca
    12001 tcggtttggc cctgggggag gagatgttgt ccctgggagc tcggcgcgtg gtcctcgcgg
    12061 atctcaacga agaaaaactc cgcaaagaga gtgaacgcct cgacgcgacc tacccaggaa
    12121 aggttctggg ggtccccacg gacgtgacga accgcgaaag agtcttttcc atgatagaga
    12181 aggcgtcgga gttcggtgag gggcgcatcg acttgctcct aaacaacgcc gggttagggc
    12241 tcgccaaggc cttcgacgat accaccgaag aagactggac ctttgccttt aacgtcaatt
    12301 tctacagcgc tctgtacggc attcgggcgg tgctccccat catgcgctct caagaagggg
    12361 gcgggcacat cgccaatacc gcctcgggca tagtcttttc ccccatgccc ctccagagca
    12421 tgtacagcgc caccaaggcc gccctcatgg ccctcaccag ctccctgcgt agcgagcttt
    12481 gggacgacca catccgattg tccaccgtca ttccgggcac cgtggccact cccatctggg
    12541 acaaggcggg gggatccccc aagggcgcca tcactcccca ggaatccgcc caggggatca
    12601 taaagggact ggcggccaac gagcggatca tcatcatcac cgaagaagat aagagcggtg
    12661 cccggaatca cttcttgccg gagtacagcg aaggggtgga cgcatacttc ctggatgtgg
    12721 cccgtcggcg caaaagggga gagattgtca tctaaagccc tccgccggag gattgccctg
    12781 ccccggaaac gggggccggg gagatcccct ggggtggggc tccccttggc cctgggggcg
    12841 tagcgcccga ggcctcgtca gcgcgccgaa gacgggctcc gatccccttt gaaacccttt
    12901 cgaccaccag cgggccggtc cttctgcccg ggctaatttg acattttttc agtaaagtcc
    12961 tatgatgacg gtggccccct cttctccctc ggaggaaccc ccggtgatgt ggcgtttccg
    13021 gccctcccgg gagcccggag gggaatgggg gaggaattcg tggcggattt tctggaagga
    13081 gggatgaggc gtggaggaaa aaaaggcgag ttgtgatcgg ccggtcgccg tggtggcggc
    13141 catggaatct cccgaaccga aggatcggga aatcccgaaa aaggaggctt cgggcatgac
    13201 gattatggtg ggacgaaagg ctcccgattt taccgccccg acgtaccgcc aggggaagtt
    13261 cggcaccgtg tccttggggg agtacctggg gaagtgggtg gtggtctgct tctatcccgg
    13321 ggactttacc tttgtctgag cgacggaagt ctcggcagtt gccgagaatt acccgaagtt
    13381 ccaggccctg ggggccgaag tgatctccat cagcgtggat agtccgtttg tgcataaagt
    13441 ttggaacgac gtggagcttt ctcgcatggt ggagggggga gttccctttt ccatggcctc
    13501 cgatagcggt ggggccatcg gccgagccta tggagtctac gacgatgtgg cgggagtgga
    13561 tgtccggggg cggtttatca tagaccccga cggggtaatt cagggcatgg aagtactcac
    13621 gccccccgtg gggcgcaacg tggaggaaac cctgcgccag ctccaggcct ttcagcacgt
    13681 ccgggccacc aaggggagcg aagtgacccc ctcgggatgg acgccggggg gcaaagttct
    13741 ccgcccgggc attgatctgg tggccaaagt atggcaggaa tggaaccccc gggacgtcta
    13801 cggggagaag aagggatagg ccaagatggg gggcggtcta cggaacgccc ccatctgttt
    13861 ttccccgcca agaaggaacc ttcccccctc gggagcggcc ctcggggggc gaggccgcca
    13921 ctgtttttga tgtgcggcga atacatgagc cctatcctgt aggggcgagc gtatttttcg
    13981 cccccacgcc aagctcaaag ttttcgcccc gacgcgggcg cggggcccgg ggatttcgcc
    14041 cccacgatgg gtttgaaccc ttctccccca cgtcggccca ttgccgatct ccgggttgct
    14101 cgcccctacg ccaggcttgg ggttttctgt tttcgccgta tcacgaggtc atcgtccaaa
    14161 ccttggcgga gaaaatcgag ccgggacgac acggctccca ggccgtcggc gggcaccg
//
LOCUS       DAOOER010000046        13116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_5283_length_13116_cov_8.433198, whole genome shotgun sequence.
ACCESSION   DAOOER010000046 DAOOER010000000
VERSION     DAOOER010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 13116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13116
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5283_length_13116_cov_8.433198"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PL162_06810"
     CDS             complement(<1..194)
                     /locus_tag="PL162_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HQF91763.1"
                     /translation="MKRMLALLTGGTVGSAVQGGSATPSEGARNRLEGLLSAFFGERE
                     FQLVLREPWGSPGYDSSDLEP"
     gene            complement(431..856)
                     /locus_tag="PL162_06815"
     CDS             complement(431..856)
                     /locus_tag="PL162_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HQF91764.1"
                     /translation="MLNRMMIELFRQVRRKPFTNPFPVKHLPENLSEALEAAGRGEIT
                     LNPPVPVPEHFRGRIAYHRKACIGCKLCIKVCPANAIEYLPEEKKVEIHVDRCCFCAQ
                     CTEICPVSCLHMTEGSLNSSYERREEVVRDSETPEGPEG"
     gene            complement(868..1866)
                     /locus_tag="PL162_06820"
     CDS             complement(868..1866)
                     /locus_tag="PL162_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HQF91765.1"
                     /translation="MSVALFLFKIVAGVILIKLSVLLALGYEGVDRIISARMQNRVGP
                     PLYQPFYDVLKLLGKETIVPRRALGWAFEGAPWLAATAAALVILYVPMGSLPPVLGGE
                     GDLILVLYLFALSAVAMVVGGFASGSPYANIGAQREMTLMMSYETPLAMVACTLAWAA
                     LHRGLPGAPFSLETHMATSLWSVLGGMGLCGMGLLLLALLAVVPAEVGKVPMDIPEAK
                     TEILEGLISEYSGRNLALLKITFAVRTLAMCAVLTSLFFPWSLGDAMALPPYLAYPVD
                     FVWFWVKVLLVQTVGVTLVRTALGRYKIWQASQFYWVQAAGIALAGMLLITLDVGL"
     gene            complement(1863..3143)
                     /locus_tag="PL162_06825"
     CDS             complement(1863..3143)
                     /locus_tag="PL162_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HQF91766.1"
                     /translation="MATGEGKTYKVPIGPVHVGLKEPITAWLDVDGERVVNATIRPGA
                     IHRGIEYMARERNPIQVIYLAERICGICSFAHITAFVRAVEDAAKIPVPLRAQYIRTI
                     LLELERLHSHILWAGVACYTIGFDAAFHLGMQLREQIMDVLEIMTGNRVNYGVGTVGG
                     VRRDITPAVASAIHAMANFYRIHFRTFVDAVFEDPVVKARMRGVGVLSAEDAVRCCAL
                     GPTARGSGLRLDLRHSAPYEAYGDIVVKPVVPQDIFGEVHGDVFDRFAVRALEVYQSL
                     DIIEKAMEGLPEGDLMWEPKLPKLLAYLKQAEGEGFAALEAPRGDDTHVVRLVKGDEN
                     VTSWKVRAPTYSNAVSWPLMFRQQELADVPLIINSIDPCISCMERVLTTDRSDGSSRV
                     LEGKHLSDLCRAKSRAWGVASDLPRTGTARRMGA"
     gene            complement(3160..3699)
                     /locus_tag="PL162_06830"
     CDS             complement(3160..3699)
                     /locus_tag="PL162_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HQF91767.1"
                     /translation="MTVAYNARFVPAAFPDVLKGLLGDRWKEVRVHDRPGGKEGAVQY
                     REYWFDLAREELLPLVDALSSLDAVHFHVLSGDDMGDCVALVYHFSLGSAAGRGARVG
                     VFPRVCLPKDDLSVPSLWSRIPGVEYSEREMREFLGVTFEGLPNTASIFLPEDWDENV
                     KPWRRDGAGLAPEHVRELS"
     gene            complement(3852..4304)
                     /locus_tag="PL162_06835"
                     /pseudo
     CDS             complement(3852..4304)
                     /locus_tag="PL162_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662525.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            complement(4315..4653)
                     /locus_tag="PL162_06840"
     CDS             complement(4315..4653)
                     /locus_tag="PL162_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HQF91768.1"
                     /translation="MWSLIDTGFGFWDVYAWIAFFLLAGGGFFYLRSQGRKDYRRGTD
                     QDDIFYGGNPVPEDGEEITVPASSAYWGFTRALEGWYKGLTAWHSGMATEYLGYMVLV
                     MGLLALAVVR"
     gene            complement(4655..6223)
                     /locus_tag="PL162_06845"
     CDS             complement(4655..6223)
                     /locus_tag="PL162_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HQF91769.1"
                     /translation="MTIWREHLPALLLALPLLGAFAAPVAGHFGSRARNVWFVSITAL
                     TALVAWGLWQQVSASGIVVYVMGAEHSRLTLPSGMALPVRILLEVDAMSALMAFMGAI
                     AALAGALFSVRSMERFSGLDQFVALYLLLTAGMLGMEITGDLFNFFVFLEIASVASCG
                     LVAFWRDRPEAVEASFKYMLLSTVGALMVLVAVAFFYGRYNTVAMAGVASHLQLGLAE
                     KAALVFLVSALAMKCGTVPMHMWTPDAYAEAPAGVTCLLVAVSQAALYGLFRVCFTLY
                     GASLGSSAVAWALIIMGLLSMFIGVTMAVIQKDVKRLMAYHSVSQVGYMLLGMGVALL
                     VLSDPQKMAAYGAMALQGAVYHLFNYTMYKGLLFLVAGALAYAAGSRNLNDLGGLGRR
                     MPQTAALFVIAAAAIAGLPPFNGFVSKLMIYESSFAVHPLLPVVAMVTSMLTLASFVK
                     VFQTAFLGPEKAALREVQEVPGFMRGGMALLAVVVLVLSLFPTWSVGSFVEPAARALM
                     DRAAYVGAVLGGGL"
     gene            complement(6220..6570)
                     /locus_tag="PL162_06850"
     CDS             complement(6220..6570)
                     /locus_tag="PL162_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter subunit C"
                     /protein_id="HQF91770.1"
                     /translation="MIGNLPYFAVAALVIFGLFGAIFGRNLVKIAVAVSLVEAATNLF
                     IIALGYRNGGSIPVFTQAGGYADYMVLPTPQALTLTAIVIGMATTALLLSLIMLLYRH
                     YGTLDVREIRRLRG"
     gene            complement(6560..7063)
                     /locus_tag="PL162_06855"
     CDS             complement(6560..7063)
                     /locus_tag="PL162_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HQF91771.1"
                     /translation="MRPLSVIVRTVCNAFAWFVAVFGVYVIVHGHLSPGGGFQGGAVV
                     ATFMALLLVSHGATKCLGWVRTNFFYAFEHVGLLTFIAAGFLGIGVTFFYNSLALSGG
                     LFGSAVPLGPNGGSLNTSGTIALMNCAVGIEVVGGLSMILLYMVQGVFHIDKPTLRGK
                     EEAGHDR"
     gene            complement(7063..7335)
                     /locus_tag="PL162_06860"
     CDS             complement(7063..7335)
                     /locus_tag="PL162_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91772.1"
                     /translation="MKFRVFFVIAILVVGGLFWGAADAVHPFGNPGDVPMDGYFLSHA
                     QRERGVNNVVTSIVFDYRGFDTLGEAAVLFTAVSSVAALFRGKGWR"
     gene            complement(7332..7640)
                     /locus_tag="PL162_06865"
     CDS             complement(7332..7640)
                     /locus_tag="PL162_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HQF91773.1"
                     /translation="MPQNDLVHGAVLVLIVVAGFFAIWFRDLLSSIISLSAVSLLLAV
                     EFYILQAPDVAIAEAAIGAGLSTAIYIVALRGCRKAPSGGSLSPEEATPEPGKEEPKP
                     "
     gene            complement(7640..8020)
                     /gene="mnhG"
                     /locus_tag="PL162_06870"
     CDS             complement(7640..8020)
                     /gene="mnhG"
                     /locus_tag="PL162_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HQF91774.1"
                     /translation="MSAFLMFLILAPLLAVGVAFNILGAVALYRFPDVYTRMHGTTKC
                     TTFGTLFIAFGALAWSLGRLAATGDGRFITMALHVGVAAVALLVTNATGAHAIARAAH
                     RSGVGPRQAVVDRLAEDRRKGGEA"
     gene            complement(8017..8283)
                     /locus_tag="PL162_06875"
     CDS             complement(8017..8283)
                     /locus_tag="PL162_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HQF91775.1"
                     /translation="MNVFLFSWSAGILGVAAVLVLGRLMGGPTIPDRVVALDTMNTLA
                     VAIMILLAVVYDSVVMVDVAVVYAALSFVTTLFIARHLEGGTGR"
     gene            complement(8280..8768)
                     /locus_tag="PL162_06880"
     CDS             complement(8280..8768)
                     /locus_tag="PL162_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HQF91776.1"
                     /translation="MCIFLLSVVTYLLLVWSGGPIEPAEVVIAVVLGGVLARAGRSWA
                     EGESWFPRSFSFIRLGRFLFYLFGPFAVALFKANIDVALRVISGEIRPGIVKVPSHQE
                     GDGALTLLANSITLTPGTLTVDVDEESRSLYVHWIYVTDPSPAEEEIYGSFGEWARRI
                     SA"
     gene            9083..10024
                     /locus_tag="PL162_06885"
     CDS             9083..10024
                     /locus_tag="PL162_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQF91777.1"
                     /translation="MEKILITGAGGQIGSELAWLLRTRGSSDAVVVSDVRDLSEELGG
                     PAEVLDVLDGKRLGDLVETHRFDTIFHLAAILSATGEKNPQRCYRINADGLFNVLEVA
                     REYGVKKVIVPSSIAAFGPSTPDSPAELTIQRPTTMYGVTKVFGELMADYYFAKYGLD
                     VRSVRYPGIISWKTEPGGGTTDYAVAVYYEALRTGRYAYFVREDTVLPMMYMPDALKA
                     LVDLADADGSRLRHRTYNVASMSFSAGELTEAVRRQLPDFRATYEPDQRQAIADSWPR
                     HLDDRAAREEWDWAPAWDIDRMSRDMVENLRAKFAPR"
     gene            complement(10184..11086)
                     /locus_tag="PL162_06890"
     CDS             complement(10184..11086)
                     /locus_tag="PL162_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HQF91778.1"
                     /translation="MAAFLGDLIAHNKRLSLLLFGVMALFLGGLGALFGEYYGNWQSG
                     LTVALVGSVIYFLVSWFLGDAMILSTSHAREISHDEAPQLWNVVEELAIASGNPMPRV
                     FIIEDDALNAFATGRDPSRASVAVTRGLLEKLNREELQGVMAHEMSHVRNYDIRFAML
                     MAVMVGMVVFLCDVFLRSLFWGGRRRRGNDKGNAVAMVLALVLAVLAPFFATLIQLAV
                     SRQREYLADASAAELTRNPRGLISALEKLSTDPAELEGANRGTQHLFIVNPLKGKRQG
                     KDSLFSTHPSLESRIERLRELTIS"
     gene            complement(11090..11671)
                     /locus_tag="PL162_06895"
     CDS             complement(11090..11671)
                     /locus_tag="PL162_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HQF91779.1"
                     /translation="MTMGWAAGVLLALALGGVILYNGLVRKRNDVENGWSQIDVQLKR
                     RHDLIPNLVATVQGYARHEKELFERVALLRSQALGASDLAGRLRSEGALSGAMKSLLM
                     VAEAYPDLKASANFLALQEELASTENRIAFARQYYNTAVNRYNTATETFPGNLVAKGF
                     SFGRRELWQIDDVAERAPGRIADLAGDGVTKEG"
     gene            complement(11668..12090)
                     /locus_tag="PL162_06900"
     CDS             complement(11668..12090)
                     /locus_tag="PL162_06900"
                     /inference="COORDINATES: protein motif:HMM:NF018021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HQF91780.1"
                     /translation="MDLETFTALAHRLVDEVPPELCRELSGGFLVRPEELWDGRYAIL
                     GQYIQDNLGSRVVFYHGSFRAVLGDAPAEVWEEELRETVFHEMRHHVERLCGTDELAE
                     EELRARAMEERGALRRRRFPGGGRRRRDLRGDEGGEKS"
     gene            complement(12104..12913)
                     /locus_tag="PL162_06905"
     CDS             complement(12104..12913)
                     /locus_tag="PL162_06905"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HQF91781.1"
                     /translation="MDQKRVSQEEDRASRRLEGLCEGGRVPGDEESAEGPRGLMRRKL
                     ASLWGEEGAQEICQALARRRAENRRTVERDFLGGLEVGLAIIEEEMGRGVLADPLTGA
                     LSKVQFMEAAEREFLRARRHGVPLALLLMDLDRFKQINLRFGRAVGDEVLRHVGILWR
                     RCMRGEDVMGRLDGEEFAVLLPETDLAGALSLGERLRCLVESCPLDKGALGAAASDGL
                     PGDEDLSVTVSVGVAVFRDGDPSFRAVMGRGDRALSRAMRQGMNRVESYGD"
BASE COUNT         2649 a         4420 c         3958 g         2089 t
ORIGIN      
        1 ggttccaggt cggagctgtc gtaaccgggg ctcccccagg gctcccggag gacgagctga
       61 aactccctct cgccgaagaa ggcgctgagg agcccttcca gacgattccg agcgccctcc
      121 gagggcgtgg cactccctcc ctgaacggcg ctccccacgg tacctccggt gagcagagcc
      181 agcatgcgct tcatgtcttt tccccctttc gcaatctttt cccgggcatc tagctaaggc
      241 gtcccttcgt gggcgccccg accgatcctc tccccggggg actgtccgcc gtcccctgcc
      301 ccttcccagg gtcagaaagg gggcaaagcg gagggccgga ggatcgttcg cctccggccc
      361 cattgtagcg cccccgttcc ggggtgcaac ggagtatcgg gcttttcggc cccctagcct
      421 ccgaagaccc ttatccctcc gggccctcgg gagtttccga gtcccgcacc acctcttccc
      481 gccgctcata ggacgagttc aacgaccctt cggtcatgtg aagacaggac acggggcaaa
      541 tctcggtgca ctgggcgcag aagcagcagc ggtccacgtg gatttccact tttttctctt
      601 ccggaaggta ttcgatggcg ttggccgggc agaccttgat gcacagtttg cagccgatgc
      661 acgccttccg gtggtaggcg atgcgccccc ggaagtgctc gggcacgggc acgggggggt
      721 tgagggtgat ctccccccgc ccggcggcct ccagggcctc cgaaaggttc tccggcaggt
      781 gtttcaccgg aaagggattg gtgaagggct tgcgacgcac ctgtcgaaag agctcgatca
      841 tcatgcggtt caacatggcc cgacctccta gagtcccacg tccagggtaa tgaggagcat
      901 gcccgccagg gcgatccccg cagcctgcac ccagtagaac tgggaagcct gccagatctt
      961 gtagcgcccc agggcggtgc gcaccagggt aacccccacg gtctgcacca agaggacctt
     1021 cacccagaac cagacgaagt ccacggggta ggcgaggtat gggggcaacg ccatggcgtc
     1081 gccgagggac cagggaaaga agagcgaggt gaggacggca cacatggcca gggttcgtac
     1141 ggcgaaggtg atcttgagga gggccaggtt acgaccggag tactccgaga tgagcccttc
     1201 gaggatctcg gtcttagcct cggggatatc catgggcacc ttacccactt cggcggggac
     1261 caccgccagg agggccagga gcaagagtcc catgccgcag aggcccatgc cccccagcac
     1321 cgaccagagg gaagtggcca tgtgggtctc caggctaaag ggagctccgg gaagaccccg
     1381 gtgcagcgcc gcccaggcca gggtgcacgc caccatggcc agcggggtct cgtagctcat
     1441 catgagggtc atttcccgct gggcgccgat gttggcgtag ggggagccgg aggcaaatcc
     1501 cccgaccacc atggccacgg cggacagggc aaaaaggtaa aggaccagga tcaaatcccc
     1561 ctcaccgccg aggacggggg gcagagatcc catgggcaca tagaggatga ccagggccgc
     1621 agcggtggcc gccagccaag gggccccttc aaaggcccaa cccagggccc gccgggggac
     1681 gatggtctcc tttcccaaaa gcttcagcac gtcgtagaag ggctggtaca gggggggacc
     1741 cacgcggttc tgcatgcggg cagagatgat tcgatccacc ccctcgtatc ccaaggccaa
     1801 taggacagaa agcttgatca ggatgactcc cgccacgatt ttgaagagaa agagcgccac
     1861 gctcatgctc ccatcctcct cgctgttccg gtccggggaa ggtcggaggc cacaccccag
     1921 gccctcgatt tggcccgaca aaggtccgaa aggtgctttc cttcgagcac ccgagaagag
     1981 ccgtcgctcc ggtccgtggt aaggacgcgc tccatgcagg aaatgcacgg gtcgatgctg
     2041 ttgatgatga ggggcacgtc cgccaattcc tgctggcgga acatgagggg ccaggagaca
     2101 gcgttactgt aggtgggggc tcggaccttc caggaggtga cgttttcgtc ccccttgacg
     2161 aggcgcacca cgtgggtgtc gtcccctcgg ggggcctcca gggcggcaaa tccttcgccc
     2221 tcggcctgtt tcaggtacgc caggagcttg gggagcttgg gttcccacat gagatcccct
     2281 tcggggaggc cctccatcgc cttctctatg atgtcaaggc tctggtaaac ctccagggcc
     2341 cggacggcga agcgatcgaa gacgtcgcca tggacctctc cgaagatgtc ttggggtacc
     2401 acgggtttca cgacgatgtc gccgtaggcc tcgtagggag cggaatgacg cagatcgagc
     2461 cgcaggccgc ttccccgggc cgtgggcccc agggcgcaac aacgcaccgc gtcttccgcc
     2521 gaaaggaccc ccacgcccct catgcgagcc ttgaccacgg ggtcttcgaa gaccgcgtcc
     2581 acgaaggtgc gaaagtgtat gcgataaaaa ttggccatgg cgtggatggc ggaggccacc
     2641 gccggggtaa tgtcccgccg gacccctccc acggtcccca cgccgtaatt tacccggtta
     2701 cccgtcatga tctccaggac gtccatgatc tgctcccgga gctgcatccc cagatggaag
     2761 gcggcgtcga agccgatggt gtagcacgcc acccccgccc agaggatgtg ggaatggagg
     2821 cgctccagct ccagaaggat ggtacggatg tactgggccc ggaggggcac ggggattttc
     2881 gccgcgtcct ccaccgcccg gacaaaggcc gtgatgtgcg caaaagagca gataccgcag
     2941 atgcgctccg cgaggtagat cacctgaatg ggattccgct cccgggccat gtactcaatg
     3001 ccccggtgga tcgcccccgg gcgaatggtg gcgttcacca ctcgctcccc gtccacgtcg
     3061 agccaggcgg tgatgggctc tttcagtccc acgtgcacgg gcccgatggg gactttatac
     3121 gtctttccct ctccagtggc cacggtcccc acctcctcct caggaaagtt cccgcacgtg
     3181 ctccggcgcc agtcccgcgc cgtcacgccg ccagggtttc acgttttcgt cccagtcttc
     3241 gggaaggaag atggacgcgg tattggggag cccctcaaag gtgacgccca aaaattccct
     3301 catctctcgc tcgctgtact ccaccccggg gatccgactc cacagggagg gcaccgaaag
     3361 gtcgtctttg gggaggcata cccgggggaa gacccccacc cgggcgcccc ggccagcggc
     3421 gctccccagg gaaaagtggt agacgagggc cacgcaatct cccatgtcgt ccccggagag
     3481 cacgtgaaag tgcaccgcat ccagggagct gagggcgtcc accaagggca acagctcctc
     3541 ccgggccagg tcgaaccagt attcccggta ctggaccgct ccttccttgc ccccggggcg
     3601 atcgtggacc ctcacctctt tccatcgatc ccccagaagg cctttcagga catcggggaa
     3661 cgccgcaggg acaaatcggg cgttgtacgc cacagtcata tagatttcac ctccgatggg
     3721 gcctcagaag ccgccgccgt ttcgggcgac ccctgggaag aaagggcctc cgcatcctcg
     3781 tcgaagtcgc tcgcccccag ggacgcaggc gacgccaggg aggcctcgag gtctcgccgc
     3841 tcctggcgga gcttttcgag cttgacgatg gccttggcca ctccttccat gatggcttcg
     3901 gggcgcgggg gacaaccatg gacgtagacg tccaccggaa tgatgcggtc cacggggccg
     3961 tccaggttgt aggacttgta gaagacgtcc ccggaggcac cgcagttgcc caccacgatg
     4021 accaccttgg gatcgggcat ctgggcgtag atgcggcgca gccgatcggt catgtacttc
     4081 gtcacgggcc ccgtcaccag caaagcgtcg gcgtgccggg gagtgcccac gagcttcatc
     4141 cccaggcgct cgatgtcata ccgaggggag agggtggcca cgatctcgat gtcacagccg
     4201 ttacaggatc cgctgttgca gtggaagacc cagatggact taggaagggc ctcctccgcc
     4261 aaaaggtcca gcctcggggg ccagtttttc aagcacccaa acatggctct cctcctaccg
     4321 caccacggcc aacgccagca gccccatcac gagcaccatg taccccaggt actcggtggc
     4381 catgccggaa tgccacgccg tgagcccttt ataccatccc tccagtgccc gggtgaaccc
     4441 ccagtacgcc gaagacgccg ggacggtgat ctcctcccca tcctccggga cggggttccc
     4501 tccgtagaaa atatcgtcct ggtccgtgcc gcgccgatag tccttccgcc cctggctgcg
     4561 gaggtagaaa aatcccccgc ccgccaggag gaaaaaggcg atccaggcgt aaacgtccca
     4621 gaacccaaaa cccgtatcga tcagggacca catgctagag ccctcctccc agcaccgcgc
     4681 ccacgtaggc ggcccgatcc atcagggccc gggcggcggg ttcgacgaaa gaccccacgg
     4741 accaagtggg gaagagcgaa agcacgagga ccaccaccgc cagaagggcc atgcctcccc
     4801 gcatgaatcc cggcacctcc tgcacttctc tcagggcggc cttctcgggc cccaggaagg
     4861 cggtctggaa gaccttgacg aaagatgcca gcgtaagcat cgacgtcacc atggccacca
     4921 caggaagcaa ggggtggacg gcaaacgaag actcgtagat catgagcttg gagacgaaac
     4981 cgttgaaggg aggaaggccc gcgatggcgg cggcggcgat gacaaagagc gccgccgtct
     5041 ggggcatgcg gcgccccaac cctcccaggt cgttcaggtt ccggctcccg gcggcatagg
     5101 ccagggcccc cgccaccaag aagaggagtc ccttgtacat ggtgtagttg aagaggtgat
     5161 ataccgcccc ctggagggcc atggcgccgt aggccgccat cttctgggga tcggacagga
     5221 ccagcagagc cacccccatc cccaggagca tgtaccccac ctgggagacg gagtgatagg
     5281 ccatgaggcg cttcacgtcc ttttggatca ccgccatggt gaccccgatg aacatggata
     5341 gaaggcccat gatgatgagg gcccaggcca cggcggagct ccccagggag gcgccgtaga
     5401 gggtgaagca gacccggaag agcccgtaga gggccgcttg gctcaccgcc accagcaggc
     5461 acgtgacccc ggcgggggcc tcggcgtagg catcgggggt ccacatgtgc atgggcaccg
     5521 tgccgcactt catggccagg gcggagacca ggaagaccaa ggcggccttt tccgccaggc
     5581 caagctggag atgggaagcc acccccgcca tggccacggt gttatagcgg ccgtagaaga
     5641 aggcgacggc taccagcacc atgagggctc ccaccgtgga aagaagcatg tacttgaacg
     5701 aggcctccac cgcctcggga cggtcgcgcc agaaggccac caagccgcag gaggccaccg
     5761 aggcgatttc caggaacacg aaaaagttaa agagatcccc cgtgatctcc atgcccagca
     5821 tccccgccgt gaggagcagg tagagggcga cgaattgatc gaggccggag aagcgctcca
     5881 tggaccgcac cgagaagagg gctcccgcca gggcggcgat ggctcccata aaggccatga
     5941 gggcgctcat ggcgtccact tccaggagga tgcgcacggg caacgccatg cccgagggca
     6001 gggtcaatcg ggagtgctcc gcccccatga cgtagaccac gatccccgag gcggagacct
     6061 gctgccacaa cccccaggcc accagggccg tgagggcggt gatgctcaca aaccagacgt
     6121 ttcgggcccg ggacccgaag tgccccgcca cgggcgcggc aaaggccccc aggaggggca
     6181 gcgccaacag cagggcggga aggtgttcgc gccagatcgt catccccgca acctccggat
     6241 ctcccgcacg tccagggtac catagtgtcg atagagcagc atgatgagcg acaaaagcag
     6301 cgccgtggtg gccatgccaa tgacgatggc cgtcagggtg agagcctggg gagtgggcag
     6361 caccatgtag tccgcatacc ccccggcctg ggtgaagacg gggatgctcc ccccgtttcg
     6421 atagcccagg gcgatgatga agaggttcgt ggcggcctcc accaggctca cggccacggc
     6481 gatcttcacc agatttctcc cgaagatcgc gccgaagagg ccaaagatca ccagcgccgc
     6541 cacggcaaaa tagggtaggt taccgatcat gccccgcctc ctcctttccc cgcagggtcg
     6601 gtttgtcgat gtgaaagacc ccctgcacca tgtacaggag gatcatggaa agacctccca
     6661 ccacttcaat gcccacggca cagttcatca gggcgatggt ccccgaggta ttcagggagc
     6721 ccccgttggg ccccaggggc accgccgagc cgaaaagccc ccccgagagc gccagggagt
     6781 tgtagaaaaa cgtcacaccg atgcccaaaa aaccggcggc gatgaaggtg agaaggccca
     6841 cgtgctcaaa ggcgtagaaa aagttggtgc gcacccaccc aaggcacttg gtggcgccgt
     6901 gggagaccag aagcagggcc atgaaggtag ccaccacagc ccctccctgg aaccctcctc
     6961 cgggggacag gtggccgtgg acgatgacgt agacgccgaa gaccgccacg aaccaggcaa
     7021 aggcattaca caccgtgcgg actatgacgg acaggggacg catcaccgcc acccctttcc
     7081 tcggaagagg gccgccacgg aagaaacggc cgtgaagagc accgccgcct ctcccagggt
     7141 gtcgaagccc cggtagtcga agacgatgga ggtcaccacg ttgttcacgc cgcgctcacg
     7201 ctgggcatgg ctcaggaagt acccgtccat gggaacgtcc ccggggtttc cgaagggatg
     7261 caccgcgtcc gccgcccccc agaagagccc tcccaccact aagatggcga tgacgaaaaa
     7321 aacacgaaac ttcatggctt cggctcctcc tttccgggct cgggggtggc ctcctccggc
     7381 gaaagggatc ctccagaagg ggccttacga cacccccgca gggccacgat gtaaatcgcc
     7441 gtggagagtc ccgcgcctat ggccgcttcg gcgatggcca cgtccggagc ctggaggatg
     7501 taaaactcca cggccaagag caggctcacc gccgagaggg atatgatcga cgagaggagg
     7561 tcccggaacc agatggcaaa aaatcccgcc accactatca gcaccaacac cgccccgtgg
     7621 accaggtcgt tttggggcat tatgcctccc ctcccttccg acgatcttcc gccagccgat
     7681 ccaccacggc ctggcggggc cccacgccgc tacggtgcgc cgcccgggca atggcatggg
     7741 ccccggtggc gttagtcacc agcagggcca ccgccgccac gcccacgtgg agcgccatgg
     7801 tgataaagcg gccgtctccc gtggccgcca ggcgccccag ggaccaggcc agggctccga
     7861 aggcgatgaa aagggtgccg aaggtggtgc acttggtggt gccgtgcata cgggtgtaca
     7921 cgtcggggaa gcgatagagg gccaccgccc ccaggatgtt gaacgccacc cccaccgcca
     7981 ggaggggggc caggatcaaa aacatgagaa aggcgctcat cgtcccgttc ccccttccag
     8041 gtgacgggcg atgaagagcg tggtgacaaa cgacagggcc gcatagacca ccgccacgtc
     8101 caccatcacc accgagtcgt agaccaccgc cagaaggatc atgatagcca ctgccagggt
     8161 gttcatggtg tccagggcca ccaccctgtc gggaatcgtg gggcctccca tcagacgccc
     8221 cagaaccagc accgccgcta cccccaggat ccccgcgctc caggaaaaca aaaagacgtt
     8281 catgcgctga tcctccttgc ccactccccg aaggaaccgt agatttcctc ttccgccggg
     8341 gagggatcgg tgacataaat ccagtgcacg tacagcgacc ggctctcttc atccacatcc
     8401 accgtgagag tgccgggggt gagggtgatg gagttcgcca acaacgtcag cgctccgtcc
     8461 ccctcctgat gcgaagggac cttcacgatg ccgggacgga tctcaccact gattacccgc
     8521 agggccacgt cgatattggc cttaaagagg gccacggcaa aggggccgaa gaggtaaaag
     8581 agaaacctcc ccagccggat gaagctgaag cttcggggaa accaactctc cccctcggcc
     8641 cagctgcggc ccgcccgggc aaggactccg cccaggacca ccgcgatgac gacctccgcc
     8701 ggttcgatgg gacctccgct ccaaaccagg agcaggtagg tcacgacgga gagcagaaag
     8761 atacacaccc acgcccacct ccttcgaaaa aagaccgcca aacctcaccc ctacggcccc
     8821 gcgccctccg ccgcacgacg gaaggacgcc cgtaccgggt tcacccatgg agtcattgta
     8881 ctacccaaag cttgtgaatg ctttctttat ctcaaaaaag gggctcccac tggaaaaaaa
     8941 tcccccgcca gaaagaggaa aacgcctctc cggggaaggg gacccgggaa ggggagcgtc
     9001 gcctcttttc gaaaagccgg tggatggaca acggccttac tgatacaatg aggccatcct
     9061 gcaaggaggt ggatctctta ccatggagaa aattctcatc accggcgccg gaggacagat
     9121 cggctcggag ttggcatggt tgctgcgcac caggggaagc tcggacgctg tggtggtctc
     9181 ggatgttcgg gacctttccg aggagctcgg aggtcccgcc gaagtcctgg atgtcttgga
     9241 cggcaaacgc ctgggagatc tggtggagac ccatcgattc gacacgatct tccacctcgc
     9301 ggccatcctt tccgccacgg gggagaaaaa tccccaacga tgctatcgca tcaacgccga
     9361 cggcctcttc aacgtactgg aggtcgcccg ggagtacggc gtaaagaaag tcatcgtccc
     9421 cagctccatt gccgcctttg gcccctccac tccggacagc cctgcagaac tcaccatcca
     9481 acgtcccacc accatgtacg gcgtcaccaa ggtcttcggc gagctgatgg ccgattatta
     9541 ctttgccaag tacggcctgg acgtgcggag cgtgcggtac cccggcatca taagctggaa
     9601 gaccgaaccc ggcgggggca ccacggacta cgccgtggcg gtctactacg aagccctccg
     9661 gacggggcgt tacgcctact tcgtgcggga agacaccgtg ctccccatga tgtacatgcc
     9721 cgacgccctg aaggccctag tggacctcgc cgacgccgat ggatcccggc tgaggcaccg
     9781 cacctacaac gtggcctcca tgagcttttc cgccggagag ctcaccgagg ccgtccggcg
     9841 ccaattgccg gatttccggg ccacctacga acccgaccag cggcaggcca tcgccgactc
     9901 ctggccccgc cacttggacg accgggcggc ccgggaggag tgggactggg cccccgcatg
     9961 ggatatcgac cgcatgtccc gggacatggt ggaaaacctc cgggccaagt tcgccccgag
    10021 gtagagcgta gttttctccc ggaaatggcc agaggtgggc cagctttccc cgcagcgaag
    10081 aagggcgctc ggcccccaca cacgttcaaa gggccgcacc tccggaagat cccgggggcg
    10141 cggccttttt cgacagaaca acagaagttc ccccgcctca gtttcaggag atcgtcagtt
    10201 cccgcaaccg ctcaatccgg ctttccagcg aagggtgggt gctgaagagg ctgtccttgc
    10261 cctggcgctt gcccttgagg gggttcacga tgaagagatg ttgcgttccc cggttggccc
    10321 cttcaagttc cgccggatcg gtggaaagtt tttccagggc ggagatgaga ccccggggat
    10381 tgcgggtgag ctccgccgcc gacgcgtccg cgagatattc ccgctggcgg ctcaccgcca
    10441 gctggatcag ggtggcaaaa aagggcgcca gcacggcgag aaccagcgcc aacaccatgg
    10501 ccaccgcgtt gcccttgtcg tttccccgac ggcggcgccc cccccagaag agggaccgca
    10561 aaaacacgtc gcagagaaag accaccatgc ccaccatgac cgccatgagc atggcaaagc
    10621 ggatgtcgta gttccgcacg tgggacatct cgtgggccat gacgccctgg agctcttcgc
    10681 ggttgagctt ttccagcaaa ccccgggtca ccgccacgct ggcccgggaa gggtcgcgcc
    10741 ccgtggcaaa ggcgttgagg gcatcgtctt cgatgatgaa cacccggggc atgggattac
    10801 cgctggcgat ggcgagctcc tccaccacgt tccagagttg aggagcttcg tcgtgagaaa
    10861 tctcccgggc gtggctcgtg gagagaatca tggcgtctcc caggaaccaa ctcacgagaa
    10921 aatagatcac gctccccacc agcgccaccg tcaggccgct ctgccaattt ccgtagtact
    10981 ccccaaagag cgcccctaat ccccccagaa aaagggccat gaccccgaag agcaggaggc
    11041 tcagacgctt gttgtgggca atgagatctc caagaaaggc ggccatggtt caaccctcct
    11101 tcgtgacgcc gtcacccgcc agatcggcga tccgcccggg ggcccgttcg gcgacgtcgt
    11161 cgatctgcca gagttcccgg cgaccgaagg aaaagccctt tgccacgaga tttcccggaa
    11221 aggtctccgt cgccgtgttg tatcggttca ccgccgtgtt gtagtactgc cgggcaaagg
    11281 ctatgcggtt ttccgtgctc gccagctctt cctgcaacgc cagaaagttg gcgctggcct
    11341 tgagatccgg ataggcttcc gccaccatca gcagagattt catggccccc gacagggccc
    11401 cctcgctccg aaggcgcccg gcgagatcgg aagcccccag agcctgggag cgcaagaggg
    11461 ccacccgctc gaagagctcc ttctcgtgcc gggcgtagcc ctgaacagta gccaccagat
    11521 tgggaatgag atcgtgccgt cgcttgagct ggacgtcgat ctggctccaa ccgttttcca
    11581 cgtcgtttct ctttcgcacc aggccgttat acaggatgac gccccccagg gccaacgcca
    11641 gcagaactcc cgccgcccaa cccatcgtca tgatttctct cccccctcgt cccctcgaag
    11701 atctcttctc cggcgtcccc ctccggggaa gcggcggcgc cttaaggccc cgcgctcctc
    11761 catggcgcgg gcccggagct cttcttccgc cagctcgtcg gtgccacaga ggcgctccac
    11821 gtgatgcctc atttcgtgaa aaaccgtctc ccgaagctct tcttcccaaa cctccgccgg
    11881 agcgtcaccc aggacggccc gaaacgagcc gtggtaaaaa accacccggc tgcccaggtt
    11941 gtcctggata tactgtccca atatggcgta ccgcccgtcc cacagctctt cggggcgcac
    12001 cagaaagcct ccggagagct ctcggcagag ctccggggga acttcgtcca ccaggcggtg
    12061 agccagggca gtgaaagttt ccagatccac gatcccttcc cctctaatct ccgtaggatt
    12121 ccacccggtt catgccctgg cgcatggccc ggctcagggc tcggtccccc cgtcccatca
    12181 cggcgcgaaa gctcgggtcc ccatcccgga agaccgccac acccacgctc acggtgaccg
    12241 aaaggtcttc gtcgccggga agcccgtcgg acgccgccgc ccccagagcc cctttgtcca
    12301 gggggcagga ttccaccaga cacctcagcc tctcccccag cgacagggcc cccgccagat
    12361 ccgtctcggg caagagcacg gcaaactcct cgccgtcgag gcgccccatc acgtcttccc
    12421 ctcgcataca ccgtcgccag aggatcccca catgcctcaa cacctcgtcc cccacggcgc
    12481 gaccgaagcg cagattgatc tgtttgaagc ggtccagatc catcaacagc aacgccaggg
    12541 gcaccccgtg tcgccgggca cggagaaact ccctctcggc ggcttccatg aactgcacct
    12601 tagacaaggc ccccgtgagg ggatcggcca ggactcccct tcccatctcc tcttcgatga
    12661 tggcaagccc cacttccagc ccccccagaa agtcccgctc tacggtgcgc cggttttcgg
    12721 cccgacgacg ggccagagcc tgacagatct cttgggctcc ttcctctccc cagagggatg
    12781 ctaacttgcg gcgcatcagc ccccgggggc cctcggccga ttcttcgtcg cccggaaccc
    12841 ttccgccctc acaaaggccc tctaatctcc gcgatgcccg atcttcttcc tgcgataccc
    12901 gtttttgatc catgattcgc atcctttcgg cgccctcttt cgggcgcgcg ccccggggcc
    12961 tggggacaaa aaggcccggg gcctcggcgt cagatcgccc ctcggggatc tacgatctcc
    13021 tcgccccggg gaaataatag tttcttaaaa aacaacaccg atatcgcgtc gaggttcatg
    13081 ccccgtcttt tagttaacct actttgccca aatctc
//
LOCUS       DAOOER010000047        12710 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_5499_length_12710_cov_8.563493, whole genome shotgun sequence.
ACCESSION   DAOOER010000047 DAOOER010000000
VERSION     DAOOER010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 12710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12710
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5499_length_12710_cov_8.563493"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            531..>1855
                     /locus_tag="PL162_06910"
     CDS             531..>1855
                     /locus_tag="PL162_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HQF91782.1"
                     /translation="MSNAVEQRENWGSRFGFIMAAAGSAVGLGNIWKFPYLAGANGGA
                     AFVVIYLAIVLAIGVSVMLAEFTIGRASGLNAVGAFKKLAGGAWPLVGWLGVLAGFLI
                     LSFYAVVAGWTIKYMIISFTGLMETAKAGQAGAAFGAFLNASTEQVIYQVLFMVATIV
                     IVHQGIGGGIEKACKVMMPALFVILLVLIVRANTLEGAGKGLEFYLMPDFSKVTGGTF
                     LAALGQAFFSLSLGMGCMITYGSYLSKSESLPFCAVVVPLIDTAVAFLAGLAIFPAVF
                     AFGMEPGAGPGLTFVTLPAVFAQMPMGEIFSCLFFLLLFFAAITSSISLLEVCVAYFK
                     DEMGWSRPMASWVLGGAITLLGIPCALSLAEGTPWMIAGKSFFDSVDFLSSNVCLPLG
                     GIFIALFVGWFWLEPARKEATNNGKISFSWEPLWIWSCRLVAPLAIAYI"
     assembly_gap    1856..1955
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     regulatory      1985..2075
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2191..2601
                     /locus_tag="PL162_06915"
     CDS             2191..2601
                     /locus_tag="PL162_06915"
                     /inference="COORDINATES: protein motif:HMM:NF038093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HQF91783.1"
                     /translation="MAERPEVFTNNAYLARRVAGECRFVEGSAFDVLVALRDRIHSGA
                     RLLTHPLCGNLRPYQQPFRSALLAPPSEGEPLSVCPSSLALVEEALAVYRSCQGRWAL
                     PGKIPAAVEEDYACVDAALMQDSLERHGLWRGAC"
     gene            2637..2954
                     /locus_tag="PL162_06920"
     CDS             2637..2954
                     /locus_tag="PL162_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HQF91784.1"
                     /translation="MIEVTKDNYEAEVLQSAMPVVVDLWGPKCGPCLALLPSVEELAQ
                     EYEGKVKFCKLNVVENRRQVISLKVMGVPTFLFYKGGEVQDRLTGGDATIEGIRERTQ
                     KLL"
     gene            2981..4270
                     /locus_tag="PL162_06925"
     CDS             2981..4270
                     /locus_tag="PL162_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HQF91785.1"
                     /translation="MKLELHKVKVTGVQWGPVTELRNGVLTVNKAELLAKVSGDERLA
                     SLDADLALPGEKTRIVPVKDVVEPRCKLEGKNEVFPGFIGDVESVGMGKTLVLDGAAV
                     VTCGKIVGFQEGIVDMSGPGAQYTPFSQTNNVVLIFTPAEGLERHDYEEAGRLAGFKA
                     AHYLASAVKNSVKADVVETFDFPTLAQAMAAHPGLPKVAYLYMLQTQGLLHDTYVYGV
                     DAKRIIPTLICPTEIMDGAIVSGNCVSACDKNSTYSHLNNPVIRALYERHGKDYNFVG
                     CIITNENVTLADKKRSSSYATKLAKMLGVDGMVITEEGFGNPDTDLIMNCRKAEALGI
                     KTVLVTDEYAGRDGASQSLADACKEADALVTGGNANEMILLPPMEKVIGFPDFANVIA
                     GGFQGSLHEDGAILVELQAITGATSELGYSKISAYTM"
     gene            4332..4808
                     /gene="grdA"
                     /locus_tag="PL162_06930"
     CDS             4332..4808
                     /gene="grdA"
                     /locus_tag="PL162_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_083775795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:4461..4463,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HQF91786.1"
                     /translation="MGKLNGKKVLLLGERDGVPGPAMEACFKDTGAEVVFSVTECFVU
                     TAAGAMDLQNQQRVKDAAEKFGVENVVVILGSSDAEGAEIYAETVANGDPTFAGPLAG
                     ASLGLAVYHVFDPEIRAEIDDAVWEEQVSMMEMVLDPEALAAAVKSMREQYSKFTL"
     gene            4850..6160
                     /gene="grdB"
                     /locus_tag="PL162_06935"
     CDS             4850..6160
                     /gene="grdB"
                     /locus_tag="PL162_06935"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030700 - glycine reductase complex
                     [Evidence IEA];
                     GO_function: GO:0030699 - glycine reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:5897..5899,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HQF91787.1"
                     /translation="MTFRVVHYINQFFAGIGGEEKAGEVLPELRQGVVGPGAALAAEL
                     KGQAEVVGTIICGDGYFAEHMEEATAKILELLNSCKPDILIAGPAFNAGRYGTACGAV
                     CDAAAKQLGIPTVTGMYKENPGVDMYRKNTFIVSTADNARGIKEAVPTMAKLALKLLK
                     KETVGLGPEEGYLERGVRRNYFLEETGAQRAAAMLAKKLRGEPFVTEYPMPVFDRVDP
                     NPAVKDLKNATIALVTSGGICPKGNPDHIEASSATKYGEYDISTFAALSEADSETAHG
                     GYDPTYANKEPNRVLPVDVLRDMEKEGVFKKLYDKYYTTVGNGTPVANAKRFGAEIGQ
                     KLKKDGVSAVILTSTUGTCTRCGATMVKEIERAGLPVVHICTIVPISLTVGANRIVPA
                     VAIPHPLGDPSRTLEEEKVIRRKLVEKALRALQTEVSDQTVFAE"
     gene            6288..7832
                     /gene="grdC"
                     /locus_tag="PL162_06940"
     CDS             6288..7832
                     /gene="grdC"
                     /locus_tag="PL162_06940"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit beta"
                     /protein_id="HQF91788.1"
                     /translation="MSKVAIKGYAYCLNHTPELGYHYGNTPFVERKAKGETEFLQALP
                     RHFQSYDQAREYAPNQSYIGGFDLDALAAHPTPWTDHRLAGASRFGAFGEIMPEDEFL
                     GLMDICDVFDLFWLESAFAAGVREKLAVHPLMRPDILARLEKGHPREEIQKEVAEAHA
                     LAVYFQGQVVGCCRRGHEVDECLEAYVLLENIACKAGGVLSLLHLLKNTALPPEEVDF
                     IIECSEEAAGDMNQRGGGNFAKAVAEIAGCVNASGCDVRSFCAGPVNAVIAAASQVAA
                     GARKNCVVVAGGAIPKLFMNGRDHVKKSLPALEDCLGNFALLFAPEDGVSPVLRLDAL
                     GKHTVGAGASPQAVTGALVSTPLEKAGLSFADVDKYAAELHIPEITLPAGAGDVPLAN
                     FKMIAALAVTKKQLEKAQIMDFVKERGVPGFAHTQGHIPSGVPFMGHACERIKAGSMK
                     RAMIIGKGSLFLARLTNLADGASFLIEAPSAPTAPAGAVTKDEVKNLLLEALGDLAAS
                     LAADKA"
     gene            7842..8996
                     /gene="grdD"
                     /locus_tag="PL162_06945"
     CDS             7842..8996
                     /gene="grdD"
                     /locus_tag="PL162_06945"
                     /EC_number="1.21.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     component C subunit alpha"
                     /protein_id="HQF91789.1"
                     /translation="MTSQNTKQLVGEALRELVEVARHGAPRVRVGLLALGSELGPEEM
                     VRGGALAQEASPDVTVVMIGPRVPGYDQLEWIETPPCEADVSQAMEDALDRGAVAGAV
                     ALHYPFPLGVATIGRVLTPARGKPMIVASSTGTTSVHRVESMIRNALYGVATAKALGM
                     ARPTVGILNVDGAQTVYRALESLRDKGYDLAFGSSVRKDGGAVLRGNDILAGAVDVCV
                     TDTLTGNVLMKMFSAYSTGGSYEALGWGYGPSAGEGWQRVVSIISRASGAPVIANAVR
                     YTADVARGGLPARVAQELDAARKAGLDALLEASRPKAVCADEVTCPPAEPTDEEIHGV
                     DVLAVEDAVRELWKAGIYAESSMGCTGPVVKLNARHAEKGHEVLKKAGYC"
     gene            9415..10788
                     /locus_tag="PL162_06950"
     CDS             9415..10788
                     /locus_tag="PL162_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HQF91790.1"
                     /translation="MEAIMKVNDMVNGFVWGPPMLVLFVGTGVYLTFLLGFPQFRYFG
                     FMFKEVLGKLGKKSEGEGSISSFAALATALSATVGTGNIAGVATAMHLGGPGALVWMF
                     ISAFFGMTTKFCEVSLAVHFREKDSAGAWRGGTMYILEKGANMKWLAWLFALFAFLAS
                     FGIGCAVQANSTSEGIMQGFGVPHLYTAIALAVLTAMVIWGGLNSISTVTTYLTPFMA
                     VFYIVGALVVIFVHSEMVIPSITSAITYAFSDPMAMPGAIAGWSIKMAMTKGVARGVF
                     SNEAGLGSAPMVHATAVVDHPVRQGVYGLFEVFVDTIVICMLTGLTIMCSGALTGQPD
                     LTGAKLSMFAFSSVLGDAGTMILAIGLALFSYSTVLGWYWYGETAAVYLFGQGVIPVM
                     KILWIILVVVGGWGGAEILTSLWDLADTLNGLMAIPNLIGLLVLSGVTRKLVKDFDEK
                     RKAGILK"
     gene            10939..12057
                     /gene="alr"
                     /locus_tag="PL162_06955"
     CDS             10939..12057
                     /gene="alr"
                     /locus_tag="PL162_06955"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HQF91791.1"
                     /translation="MSLRPTRMEVRPAALAANFAAIRAWVGPGCMPMAVVKADGYGTG
                     VMSGVRALQKEGCQRFCVATPDEAVFLRDQGVREPLLVMGASPEAAAEEYVARDIAAA
                     LTDLRFARAMSAAAKRRGKPAQLHLKVDTGMGRIGFLPQEIPGVLEALKDLGGVELEG
                     LFTHFATADERHREFTETQFRRYGDVLEMLRGAGLGVKIRHVCNSAGIMNFPAMHLDA
                     VRPGLILYGMEPSSWCDRPFALQEVYTVKTAVAVLRELVPGWGVSYGLRYSARGHDRV
                     AVLPIGYADGYSRVLTGKASVLVRGVRAPVVGSICMDQTMVDVSHIPGVDVGDEVVLV
                     GSQGSERLSPEEVAGWLGSINYEIPCMFTPRVPRVAVA"
     gene            12196..>12710
                     /locus_tag="PL162_06960"
     CDS             12196..>12710
                     /locus_tag="PL162_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQF91792.1"
                     /translation="MNLSKRIQDMQESPIRKLVPIAQKAKKEGRKVYHLNIGQPDIPT
                     PPAFFEAVKNFDAKVLEYSFSQGSPDLIRAMLPYYASFGINFEERDILVTNGGSEAIL
                     FSLIAVADPGDEVLVPEPFYTNYNGFATPVGVKVVPITTTAEEGFALTSKDRIVSRIT
                     PRTRAILLSNPG"
BASE COUNT         2250 a         3911 c         4140 g         2309 t
ORIGIN      
        1 ggcttcatgg tgcattcccc ctttttgctg cgtgagccct ctcctagggc ggtcccctcc
       61 ggcgggcccg ccggaggctg ggcctccggg gaaagggcgt cctctgcgcc tggtctcttg
      121 tcactagata taccacgaat gtccggagct cacaacaaaa aagtccctcc gcgcgggagt
      181 gaggggcaca acttcgccct cgacgttggc cgagggggtg cggcaaagtt cggtgaggcc
      241 tctccgattc tagggttcgt tgaaaaaaca caaggcttcc ccggggaaga acttttctgc
      301 tttcccggcc attgcgcatc cgggaagatg gcactaaaat ggacggataa gagctcggga
      361 ctttggcgta ctggatgggg tgtatccggg cgtcgggccc ggggggcctt cacggaagga
      421 ggtggcgggc ggggattgga gcgaagggag cggggcgtat cggccagaga tgtggttttg
      481 ataagaattc atgatgtctc tgaaattttt cataattgag gagggacaca atgagcaacg
      541 cggtcgaaca gagagagaat tggggcagcc gttttggctt catcatggcc gctgcggggt
      601 cggcggtggg gctgggaaac atctggaagt tcccctacct ggcgggggcc aacggcggcg
      661 ccgcctttgt ggtgatctat ctcgccatcg tccttgccat cggcgtcagc gtcatgctgg
      721 cggagttcac catcggccgg gcctcggggc tcaacgccgt gggggccttc aaaaagctcg
      781 cggggggggc ctggcccctg gtggggtggc tgggagtcct ggcgggcttc ctcatcctct
      841 ccttctacgc cgtggtggcg gggtggacca ttaagtacat gataatttct ttcactggcc
      901 tcatggaaac cgccaaggcg gggcaggccg gggcagcctt cggcgccttc ctgaacgcct
      961 ccacggagca ggtgatctat caggtgctct tcatggtggc caccatcgtc atcgtgcacc
     1021 agggcatcgg cggcggcatc gagaaggcct gcaaggtcat gatgcccgcc ctcttcgtca
     1081 tcctcttggt gctcatcgtc cgggcgaata ccctcgaggg cgcgggcaag gggctggagt
     1141 tctacctcat gcccgatttc agcaaagtca ccggcggcac cttcctggcg gccctgggtc
     1201 aggccttttt ctccctctcc ctggggatgg gatgcatgat cacctacggg agctacctct
     1261 ccaagagcga atcgctcccc ttctgcgccg tggtggtgcc cctcatcgac accgccgtgg
     1321 ccttcctggc ggggctcgcc attttccccg cagtcttcgc cttcggcatg gagcccggcg
     1381 cgggccccgg cctcaccttc gtgacgctcc cggcggtctt tgcccagatg cccatggggg
     1441 agatcttcag ctgcctcttc ttccttctcc tcttcttcgc cgccatcact tcctccattt
     1501 ccctcctgga ggtttgcgtg gcgtacttca aggatgagat gggctggagc cgtcccatgg
     1561 cctcctgggt cttgggcggg gctatcaccc tgctggggat cccctgcgcc ctctccctgg
     1621 cggagggtac gccgtggatg atcgcgggca aatccttctt tgactccgtg gacttcctgt
     1681 catccaacgt ttgtcttccc ctgggaggca tcttcatcgc cctcttcgtg ggttggttct
     1741 ggctggagcc cgcccggaag gaagccacga acaacggcaa aatttccttc tcctgggaac
     1801 ccctgtggat ctggagctgc cgcctggtgg ctcccctggc catcgcctac atcttnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnntggta caatgactcc gtcgagattc
     1981 cctgatgact gtcgcgagag agacccgcgc cgcgggcgcc gaaggggcaa gaccgtccgg
     2041 tcgaaactct caggcccaag gatcgtgaca ggaaggacct ctggatgaga cgcctggagc
     2101 gtctgccccc ccgaatcgcc ggaagcccgg cgcccttcgt tcgggcgttc tgacagagag
     2161 aggccgactc ctggcgagga ggggtttctg ttggcggagc gtccggaagt ctttaccaat
     2221 aacgcctatc ttgcccggcg cgtggccggg gagtgtcgtt tcgtcgaggg tagcgccttt
     2281 gacgtgttgg tggcgttgcg ggatcggatc cactcaggcg cccgccttct cacccatccc
     2341 ctctgcggca acctcaggcc ctaccaacag ccctttcgct ccgcgctcct cgccccccct
     2401 tcggagggcg aaccactttc ggtgtgccct tcatcgttgg ccctggtgga ggaggccctg
     2461 gcggtgtacc gctcctgcca ggggcgatgg gcccttcccg ggaagattcc cgctgcggtg
     2521 gaagaagact atgcctgtgt ggacgcggcg ctgatgcaag actccctgga gcgccacggc
     2581 ctttggcgtg gggcttgcta gaagaaaatc ttcgtcgaag ggaggtgacg gaaaagatga
     2641 tcgaggtgac caaggacaac tacgaagcag aggtgctaca gagcgcgatg cccgtggtgg
     2701 tggatttatg gggtcccaag tgcggccctt gcttggccct cttgccctcc gtggaggaat
     2761 tggcccagga gtacgagggc aaggtgaagt tctgcaagct caacgtggtg gaaaaccggc
     2821 ggcaggtgat ttcgctcaag gtcatggggg tacccacgtt cctcttctac aagggagggg
     2881 aggtgcaaga tcggctcacc ggcggtgacg caaccatcga aggaattcgg gagcgcaccc
     2941 agaagcttct ataaggggtg cgaaaggggg ggaatcgaca gtgaagttgg aattgcacaa
     3001 ggtaaaggtg acgggagtcc agtggggtcc ggtcacggag ctgagaaacg gtgtcctcac
     3061 cgtgaacaag gcggaacttc tggccaaggt gtcgggggat gagcgtctcg cctcgctgga
     3121 cgcggatctg gccctgcccg gggagaagac ccgcatcgtt cccgtgaagg acgtggtgga
     3181 accccggtgc aagctcgagg ggaagaacga ggtcttcccg ggcttcatcg gcgacgtgga
     3241 atccgtaggt atgggaaaaa ccctggtgct ggacggcgcc gccgtggtga cctgcggcaa
     3301 gatcgtgggc ttccaggagg gcatcgtgga catgtccggc cccggggcgc agtacacgcc
     3361 cttttcccag accaacaacg tggtgttgat cttcaccccc gccgaggggc tcgagcgcca
     3421 tgactacgaa gaagcggggc gcctcgcggg cttcaaggcg gcgcactatc tcgcctccgc
     3481 agtgaagaac tcggtgaagg ccgatgtggt ggagaccttc gacttcccca ccctggccca
     3541 ggccatggcg gcccatccgg ggctccccaa ggtggcctat ctctacatgc tccagaccca
     3601 ggggctcttg cacgacacct atgtctacgg cgtggacgcc aagcgcatca tccccaccct
     3661 catctgcccc acggagatca tggatggggc catcgtgtcc ggcaactgcg tctcggcctg
     3721 cgacaaaaat agcacctatt cccacctcaa taaccccgtc attcgggcgc tctacgagcg
     3781 gcacggcaag gactacaact tcgtggggtg catcattacc aacgaaaacg tcactctggc
     3841 cgacaagaag cgtagctcct cctacgccac caagctcgcc aagatgctcg gcgtggatgg
     3901 catggtcatc accgaggagg ggttcggcaa cccggacacg gacctcatca tgaactgccg
     3961 taaggccgag gccctgggga taaagacggt cctcgtcacc gacgagtacg cggggcgcga
     4021 cggcgccagc cagtccctgg cggacgcttg caaggaagcg gatgccctcg tcaccggcgg
     4081 taacgccaat gagatgatcc tactgcctcc catggagaag gtcatcggct tcccggactt
     4141 tgccaacgtc atcgcggggg gattccaggg ctctctgcac gaggatggag ccattctggt
     4201 ggagctccag gccatcaccg gggccaccag cgagctgggc tacagcaaga tcagcgccta
     4261 caccatgtga cacctggggc ccctgggggc tccaaacctt ctatcttttg acgaaaggag
     4321 ttgttctcac catgggtaag cttaacggca agaaagtgct tttgttgggc gaacgggacg
     4381 gcgtgcccgg ccccgccatg gaagcctgct tcaaagacac cggggcggaa gtggtctttt
     4441 cggtgacgga gtgttttgtc tgaaccgccg ccggagccat ggacctgcag aaccagcagc
     4501 gtgtgaagga cgccgctgag aagttcggag tggagaacgt cgtggtgatc ctgggctctt
     4561 ccgatgcgga gggcgccgag atctacgccg aaacggtggc caatggcgat cccacttttg
     4621 ccggtcctct ggctggagcc tcgttggggc tcgcagtgta tcacgtgttt gatcccgaga
     4681 tccgcgcgga aatcgacgat gcggtgtggg aggagcaggt cagcatgatg gagatggtcc
     4741 tcgatcccga ggccctcgcc gccgctgtaa aatccatgcg ggagcagtac agcaagttca
     4801 ccctgtaagc tccccgacga atcccaaaag aaggagggag gcacagaaca tgaccttccg
     4861 ggtagtgcat tacattaacc aattctttgc cgggatcggt ggcgaggaga aggcgggaga
     4921 ggtccttccc gaactgcgtc agggtgtggt aggccccggc gccgccctgg ccgccgagct
     4981 caaagggcag gcggaggtcg tgggcaccat catctgcggc gacggctact tcgccgagca
     5041 catggaggaa gccaccgcca agatcttgga actgctcaat tcctgcaagc ccgatatcct
     5101 catcgcgggc ccggcgttca acgcaggacg ctatggcacg gcctgcggcg ccgtgtgcga
     5161 tgccgccgcc aagcaactgg gcattcccac ggtgacgggg atgtacaaag aaaaccccgg
     5221 cgtggacatg taccgcaaga atactttcat cgtctccacg gcggacaacg cccggggcat
     5281 caaggaagcg gtgcccacca tggcgaagct cgccctgaag ctcctgaaga aggaaaccgt
     5341 gggcctcggc cccgaagagg ggtaccttga gcggggggtc cgacggaact acttcctgga
     5401 ggagaccgga gcccagcgcg ccgccgccat gctggccaag aagctccggg gggagccctt
     5461 cgtgacggag tatcccatgc cggtctttga ccgagtggat cccaaccccg ccgtgaagga
     5521 cctgaaaaac gccaccattg ccctggtgac ctccggcggc atctgcccca agggaaaccc
     5581 cgaccacatc gaggcttcca gcgccaccaa gtacggcgaa tacgatatct cgacctttgc
     5641 cgccctctcc gaggcggatt cggaaaccgc ccacggcggc tacgatccca cctacgccaa
     5701 caaggaaccc aaccgggtgc tccccgtgga cgtgctccgg gacatggaga aagagggcgt
     5761 cttcaagaag ctctacgaca agtactacac caccgtgggc aacggcaccc ccgtggccaa
     5821 cgccaagcgc ttcggcgccg aaatcggcca gaagctcaag aaggatggag tttccgccgt
     5881 catcctcacc tccacctgag gcacctgcac tcgttgcggc gcaacgatgg taaaagaaat
     5941 tgagcgcgca ggtcttcccg tggtccacat ctgcacgatc gtgcccatct ccctcacggt
     6001 gggggcaaac cgcatcgtgc ccgccgtggc cattccccat cccctgggag atccgtcgag
     6061 gaccctggaa gaagaaaaag tcatccggcg caagctggtg gaaaaggccc tccgggccct
     6121 gcagaccgag gtaagcgatc agacggtctt tgcggaatag gcggtcttcc gaggttcctc
     6181 ggagccccgg aagttctcac aaaacggtga agggtggagg gaagcggggc agagccccct
     6241 tcatccttca tccttcatct tttaataata aaaaggagcg tcgtcccatg tccaaagttg
     6301 ccatcaaggg ctatgcctac tgtctgaacc acacccccga gctgggatat cactacggca
     6361 acaccccctt tgtggagcgc aaggccaagg gggaaacgga gtttcttcag gccctgcctc
     6421 gccactttca gagctatgac caggcccggg agtatgcccc gaaccagtcc tacatcgggg
     6481 gcttcgacct ggacgccctg gcggcccacc ccacgccctg gaccgatcat cgcctggcgg
     6541 gggcttcccg ctttggggcc tttggcgaaa tcatgcccga ggacgagttt ctgggcctca
     6601 tggacatctg cgacgtcttc gaccttttct ggctggaatc ggcctttgcc gccggggtga
     6661 gggagaagct cgcggtccat cccttgatgc gcccggacat cctggcccgg ctggaaaagg
     6721 ggcaccctcg ggaggagatc cagaaggaag tggcggaggc ccacgccctg gcggtgtact
     6781 tccaggggca ggtggtgggg tgctgtcgtc gggggcacga ggtggacgaa tgcctggagg
     6841 cctatgtgct tctggagaac atcgcctgta aggccggagg agtgctttcc ctcctgcacc
     6901 tcctgaagaa cacggccctg ccccccgaag aggtggactt catcatcgag tgctccgaag
     6961 aggcagcggg ggacatgaac cagcgcggcg ggggcaactt cgccaaagcc gtggcggaga
     7021 tcgcggggtg cgttaatgcc agcggctgcg acgtccggag cttctgcgcc ggtcccgtga
     7081 acgccgtcat cgccgccgcc tcccaggtgg ccgcaggggc ccggaagaac tgcgtggtgg
     7141 tcgccggggg ggcgatcccg aagctcttca tgaacggccg ggaccacgtg aaaaaatctc
     7201 ttccggccct ggaggactgc ctgggcaact tcgccctgct ctttgccccc gaagacggcg
     7261 tttctcccgt gctccgcctg gacgccctgg gcaagcacac ggtgggggcg ggggcctctc
     7321 cccaggcggt gacgggggcc ctggtgtcca cccccttgga aaaggcaggg ctctccttcg
     7381 ccgacgtgga taagtacgcc gccgagctcc acatccccga gatcaccctt cccgccgggg
     7441 cgggggacgt gcccctggcg aacttcaaga tgatcgccgc cctggcggtg acgaaaaagc
     7501 agctggaaaa ggcccagatc atggacttcg tcaaggaacg gggcgtcccg ggctttgccc
     7561 acacccaggg gcatatcccc tcgggggtgc ccttcatggg gcacgcctgc gagcgcatca
     7621 aggcggggag catgaagcgc gccatgatca tcggcaaagg gagcctcttt ttggcccgcc
     7681 tcaccaacct cgccgacgga gcctctttcc tcatcgaagc cccctcggcc ccgacggccc
     7741 ccgctggggc ggtgaccaaa gatgaggtca aaaacctcct cttggaggcc ctgggggatc
     7801 tggccgcctc tctggcggcg gacaaggcct aggtgggttc catgacgtcc cagaacacga
     7861 aacagctcgt gggggaggcc ctgcgggaac tggtggaggt ggcccgccac ggcgcgcccc
     7921 gggtgcgcgt cggccttctg gccctgggga gcgagctggg ccccgaggaa atggtccggg
     7981 gaggggccct ggcccaggag gcctctccgg acgtgacggt ggtcatgatc ggcccccggg
     8041 tgccggggta tgaccagcta gagtggatcg agaccccccc ttgtgaggcc gacgtgtccc
     8101 aggccatgga ggacgccctg gaccggggcg ccgtggcggg ggccgtggcg ctccactacc
     8161 cctttcctct gggagtggcc accatcggcc gggtgctgac ccccgcccgg ggaaagccca
     8221 tgatcgtggc ctcttccacg gggaccacct cggtgcaccg ggtggaatcc atgatccgca
     8281 acgccctcta cggcgtggcc accgccaagg ccctgggcat ggctcgcccc acggtgggca
     8341 ttctcaacgt ggacggagct cagaccgtgt accgggctct ggagagcctc cgggacaagg
     8401 ggtatgacct ggcctttggc tccagcgtcc gcaaggacgg aggggcggtg ctccggggaa
     8461 atgacatcct cgccggggcc gtggacgtgt gtgtcacgga caccctcacg gggaacgtgc
     8521 tcatgaagat gttttccgcc tatagcacgg gaggaagcta cgaggccctg ggatggggct
     8581 acggcccttc cgcaggggaa gggtggcagc gggtggtttc catcatctcc cgggccagcg
     8641 gcgcgccggt catcgccaac gccgtgcgct acaccgccga tgtggcccgg ggaggactgc
     8701 cggcccgggt ggcccaggag cttgacgccg cccggaaagc ggggctcgac gcccttctcg
     8761 aagcgagccg ccccaaggcg gtctgtgcgg acgaggtgac ctgtcccccc gcagagccca
     8821 cggacgagga aattcacggc gtggatgtcc tggcggtgga ggatgcggtg cgcgaactct
     8881 ggaaggcggg gatctacgcc gaatcctcca tggggtgcac gggccccgtg gtgaagctca
     8941 acgcccgtca cgcggaaaaa gggcatgagg tgctgaaaaa agcggggtac tgctaaaacc
     9001 tcagggccgg agcccggccc catccctttt gggaaaggcc gggctccggg atctttacga
     9061 aggatcgatg ggaaaggacc tccggaggac gaccgaagac cttcccttcc ccgaggttat
     9121 tccccgaaac ccctttctcg ggtataatgc atccggagtt tgcgacgctt ccatggtcca
     9181 tggggagaga gggcgggggt ttcctcgcgc gggggcattt tgtcgaagaa gatggacaag
     9241 gcccctgagc cgaaaaaacc gaggacgggc cccttcccct ggggggggtc gggagtaggg
     9301 tgggaaggga gggatcgaag gcctgaggga tgagatgcgt atcggaatca gctttagcga
     9361 aatgcagaga cgtgctgatg ccaaaactgc agacgtgaag gaggtcattg cgcgatggaa
     9421 gcaattatga aggtcaatga catggtcaac ggtttcgtct ggggcccccc tatgttggtg
     9481 ctcttcgtgg ggacgggggt ctacctcacc ttccttctgg gcttccccca gttccgctac
     9541 ttcggtttca tgttcaaaga ggtcctcggc aagctcggca aaaagagtga gggtgagggc
     9601 tccatctcct ccttcgccgc cctggccaca gccctttccg ccacggtggg cacgggaaac
     9661 atcgctggcg tcgccaccgc catgcacctc ggcggcccgg gggctctggt gtggatgttc
     9721 atctccgcct tcttcggcat gaccaccaag ttctgcgaag tctccctggc ggtgcacttc
     9781 cgcgagaagg attcggcggg ggcctggcgg ggcggcacca tgtacatcct ggaaaagggc
     9841 gccaacatga agtggctggc gtggctcttc gccctcttcg ccttcctggc gtccttcggc
     9901 atcggctgcg cggtccaggc gaactccacc tcggagggca tcatgcaggg cttcggcgtg
     9961 cctcacctct acaccgccat cgccctggcc gttctcaccg ccatggtgat ctggggaggc
    10021 ctcaacagca tctccaccgt caccacctac ctcaccccct tcatggcggt cttctacatc
    10081 gtgggggccc tggtggtcat cttcgtccac tccgagatgg tgatcccctc catcaccagt
    10141 gccatcacct acgccttcag tgatcccatg gccatgcccg gcgccatcgc gggttggagc
    10201 atcaagatgg ccatgaccaa gggcgtggcc cggggggtct tctccaacga ggcgggcctc
    10261 ggctccgccc ccatggtgca cgccacggcg gtggtggacc accccgtgcg tcagggcgtc
    10321 tacggcctct tcgaggtctt cgtggacacc atcgtcatct gcatgctcac cgggctcacc
    10381 atcatgtgca gcggcgccct cacggggcag cccgatctca cgggggccaa gctctccatg
    10441 tttgccttta gctccgtcct gggggacgcg gggaccatga tcctcgccat tggcctggcc
    10501 ctcttctcct actccacggt gctcgggtgg tactggtacg gcgaaaccgc cgccgtgtac
    10561 ctcttcggcc agggagtcat ccctgtcatg aagatcctct ggatcatcct ggtggtggtg
    10621 ggaggttggg gcggcgccga gatcctcacc agcctgtggg atctggcgga cacccttaac
    10681 ggcctcatgg ccatcccgaa cctcataggg cttttggtgc tctccggcgt gacccgcaag
    10741 ctcgtcaagg acttcgacga aaagcgcaag gcgggcattc tgaagtaggc gcggggctac
    10801 ccgagggcgg cgttttcttg gcgggggtgg gcgtgtcggc tcgcccccgt tcctcatgga
    10861 gggcccgtcc agcggcccgg ggtgcggcaa ggtatgaagg catggaggat cgataatttg
    10921 atggggaggt gggggagctt gtcccttcgt ccgacgcgta tggaggttcg ccccgccgct
    10981 ttggcggcga attttgcggc catccgggcc tgggtgggcc cggggtgtat gcccatggcg
    11041 gtggtaaaag ccgatggcta tggcacgggg gtgatgtcgg gagtacgggc gctgcagaaa
    11101 gagggatgcc agcgcttctg cgtggccacc cccgatgagg cggtcttttt gcgggaccaa
    11161 ggggtgcggg aacccctgct ggtcatgggg gcctctcccg aggccgccgc cgaggagtac
    11221 gtggcccggg acatcgccgc cgccctcacg gaccttcgct tcgcccgggc catgagcgcg
    11281 gcggcgaagc gccggggaaa gcccgcccag ctccacctca aggtggatac gggcatgggg
    11341 cgcattggct ttctccccca ggagattccc ggggtgctgg aggcgttgaa ggacttaggg
    11401 ggagtggaac tggagggact tttcacccac ttcgccacgg cggatgaacg ccaccgtgag
    11461 ttcaccgaaa cccagttccg gcgctacggc gacgtgctgg agatgctgcg gggggcgggg
    11521 ctgggggtga agatccgtca cgtctgcaac agcgcgggga tcatgaattt tcccgccatg
    11581 cacctggacg cggtgcgccc cgggctcatc ctctacggca tggagccctc ttcctggtgc
    11641 gaccgtccct tcgccctcca ggaggtatac acggtgaaga ccgctgtggc ggtcctccgg
    11701 gagctggtgc cggggtgggg cgtgagctat ggcctgcgct acagcgcccg gggtcacgac
    11761 cgggtggcgg tgcttcccat cggctatgcc gacgggtact cccgggtcct caccggcaag
    11821 gcttcggtgt tggtgcgggg ggtacgggcc ccggtggtgg ggagcatctg tatggatcag
    11881 accatggtgg acgtctccca cattcccggc gtggacgtgg gcgacgaggt ggtgctggtg
    11941 gggagccagg gatcggagcg cctttccccc gaggaagtgg cgggatggct gggttccatc
    12001 aactacgaga tcccctgcat gttcaccccc cgggtgcccc gggtggcggt ggcctagtgc
    12061 cttttttgaa aaagtgtagt ataatacatg caggaagaac accccggtct cgggggcgga
    12121 gtctccggcg ggtgccggaa aaaaggcggg gaaggccccc ttccccggag gcatgacaag
    12181 gaggagaaga cggacatgaa tctttccaag cggattcagg acatgcagga gtctcccatt
    12241 cgtaagctgg tacccatcgc ccagaaggcc aagaaggaag ggcgcaaggt ctaccacctg
    12301 aacatcggcc agccggacat ccccactccc cctgcctttt tcgaggcggt aaagaacttt
    12361 gacgccaagg tgttggagta ctccttcagc cagggaagcc ccgacctgat ccgggccatg
    12421 ctcccctact acgcctcctt cgggattaac ttcgaggagc gggacatcct ggtgaccaac
    12481 ggcggcagcg aggccatcct cttttccctc atcgccgtgg cggatcccgg cgacgaggtg
    12541 ctggtgcccg agcccttcta caccaactac aacggcttcg ccacccctgt gggggttaag
    12601 gtggtgccca ttaccaccac ggcggaggag ggttttgccc tcacctccaa ggatcgcatc
    12661 gtcagccgca tcaccccccg cacccgggcg atcctcctct ccaaccccgg
//
LOCUS       DAOOER010000048        12104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_5807_length_12104_cov_8.302017, whole genome shotgun sequence.
ACCESSION   DAOOER010000048 DAOOER010000000
VERSION     DAOOER010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 12104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12104
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5807_length_12104_cov_8.302017"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="PL162_06965"
     CDS             complement(<1..814)
                     /locus_tag="PL162_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91793.1"
                     /translation="MRGGQACPEDDERADGDSMWKTITEVNFDEMLASILPELAAQVD
                     PRRETEFLDKEMKDLKALMGGAWQAPDLLARVPLKGGRDIWLALHVEVQDKGGENFPE
                     RMFYHHSIIRFKYLKRKKYEEELVDAAGDGGRKTAKVRKGVVDVVSLALLTAKRPGKE
                     AGHYEHASFGNELRFVYPTVKLWELDADRLEKSPNPFDLALLAAKRMIDSGRSDNKRI
                     AFLKQLGGLLELYFATNTPRFLCHEIPNFLRKSSPRRLAPSLVNESCGSLLTE"
     gene            complement(1459..2025)
                     /gene="pseH"
                     /locus_tag="PL162_06970"
     CDS             complement(1459..2025)
                     /gene="pseH"
                     /locus_tag="PL162_06970"
                     /EC_number="2.3.1.202"
                     /inference="COORDINATES: protein motif:HMM:TIGR03585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine N-acetyltransferase"
                     /protein_id="HQF91794.1"
                     /translation="MISLSYRDLTELSPEMGEQVRLWRNTPEVRSSMVRQHPISPEEH
                     AQWLEFLRRHPHRQRVRVAFVDHEPFGVVTLKDLDLAAGVSDWGMYIGDPRFRGRGLG
                     NALLFEILRWAFDESGLFRLFTSVLAVNPQALVMYLRAGFELEGCWRRHVSLGGGERG
                     DLLWMAMLRPRWEERRKTLEEALLAGVR"
     gene            complement(2158..>2605)
                     /locus_tag="PL162_06975"
     CDS             complement(2158..>2605)
                     /locus_tag="PL162_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HQF91795.1"
                     /translation="TLRRAFGVPTGLSDHTRGIHVSVAAAALGASVIEKHFTLDRTLP
                     GPDHPFAIEPPELKELVRQVREVESALGDGRKRGPAPEEREFYEKARRSVHAAVDIAA
                     GTAITEEMLCVKRPGYGIRPKLLPLLLGRVARQDVAADQWITWEMV"
     assembly_gap    2606..2705
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2706..2953)
                     /locus_tag="PL162_06980"
     CDS             complement(<2706..2953)
                     /locus_tag="PL162_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HQF91796.1"
                     /translation="MLTLRGGKRVGDGAPCFIVAEIGANHNRSLTMAKELIDAAVEAR
                     ADAVKLQIYSAETLYSRRTPRHSGYDQDLFSLIKEIETP"
     gene            complement(3000..3557)
                     /locus_tag="PL162_06985"
     CDS             complement(3000..3557)
                     /locus_tag="PL162_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91797.1"
                     /translation="MVERVGVSLYDEGEYHALVRSTPRPDLLQVPSNLLDRRFSLREL
                     ERRGEESPPPVPLWGRSLFLQGLLAQGDREPWQRVGGDVLRREEFRRALASLAEDVRM
                     SLKELALLAVGTLFCLEGVVVGAEDPRQIGEWGALWSAAGARNAPEDLEERLELLGRI
                     MPPGLADPRRWPLAEDVAPAETEKT"
     gene            complement(3573..3986)
                     /locus_tag="PL162_06990"
     CDS             complement(3573..3986)
                     /locus_tag="PL162_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQF91798.1"
                     /translation="MKTDGAMRGATKTPALILGTAQLGMAYGAANHRGMLSDVQGREV
                     LEAAWNAGVRWFDTARAYGESERRLGAFLRSLSHAERRDARVATKLAPGAGLAPGECL
                     RSLESSLEALGTGGVAVLFVHRGEEWTPTLARDLG"
     gene            complement(3983..4957)
                     /locus_tag="PL162_06995"
     CDS             complement(3983..4957)
                     /locus_tag="PL162_06995"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HQF91799.1"
                     /translation="MTCLVTGGAGFIGSHLVDALLARGERVVAVDDFSNGRPENVAHL
                     AENGNFSLVRGNVCAAAVRDEVWRRGPFSVVYHLAAEINVQKSLDDPAETFDRDVVGT
                     FHLLERVRATPCRFVFMSTCMVYDRCEDAPGIGEKSPVKPASPYAAAKLAGEALTLSY
                     HFGYGLPTVVLRPFNTYGPRQKTGGEGGVVAIFVRNALEGKSLSIYGDGTQTRDLLFV
                     EDCVDFILRAGECPEVLGQIWNAGTGRDVSINDLAALVSREVGGGAAEIGHVPHIHPQ
                     SEIAVLRCDARKARDRLGWAPRISLDEGIRRVAAWMGAPSSQASGKDL"
     gene            complement(5077..>5568)
                     /locus_tag="PL162_07000"
     CDS             complement(5077..>5568)
                     /locus_tag="PL162_07000"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HQF91800.1"
                     /translation="EGRALPVTHPEMSRFVMSVDRTTELMLQAMDLSVGGETFVFKMP
                     VVRLGDLAEVLWRRERPGDPFRMDQIGLRPGEKMFEELMTEEESHRALETESMFLILP
                     FGKNPDARVGRYPGAWVASPGEYRSDGQKVLPLEKTACLVEEMWSALGRLPDMEEPRG
                     RSL"
     assembly_gap    5569..5668
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5669..5922)
                     /locus_tag="PL162_07005"
     CDS             complement(<5669..5922)
                     /locus_tag="PL162_07005"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HQF91801.1"
                     /translation="MRIRKALVIGGTGTVGIRLVQELLAQGAETVRIFSRDEYKQYLL
                     ANAWSTEGRCRFLLGDVRDRDRLLRAMEGVEAVFHLAALK"
     gene            complement(6004..6798)
                     /locus_tag="PL162_07010"
     CDS             complement(6004..6798)
                     /locus_tag="PL162_07010"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQF91802.1"
                     /translation="MPSGERTVLVTGGCGGLGGSLSLHLAEKGWTVFPTSRDEVSAQA
                     FRERAGTRPVHPVLWRWETEEDVREGFGELRRTMDAVGAPPLGALVHTVASRKPQVEW
                     RSLCSADWEEEYLLSSVRFFEVARQCALYWHERTLPGAVVAVSSIYGHHAVDPGIYDD
                     FMMENGLFAGLPYAAAKAALEHLVRCLARLWGPWNIRVNAVAPGGVRNPERQSVAFVE
                     AYGARTPLGRLGNLEEMGGAVAFLLSSEASYVTGTVLPVDGGWDTR"
     gene            complement(6785..7501)
                     /locus_tag="PL162_07015"
     CDS             complement(6785..7501)
                     /locus_tag="PL162_07015"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HQF91803.1"
                     /translation="MTAGAPGRHFGVVPARSGSKGLAHKNILELGGHPVLAYSIEAAR
                     KSGLFREIFVATDDPHYADLAREAGAAVPFLLPLEVTGDAVSSTDALLYVWEREGTGE
                     DLLWCLQPTSPFRLPEDLTGARDLFVQFPDTACVVSGTPVDPHFFHWVLRPEENGTYG
                     MWFGDGFLKDRSLLPPVFRPNGMIKVGRREVVASRRHFFVDPLRMLETPEARSIHIRS
                     ALDFSLCCLLVEEGGAHAFR"
     gene            complement(7498..8211)
                     /gene="pseF"
                     /locus_tag="PL162_07020"
     CDS             complement(7498..8211)
                     /gene="pseF"
                     /locus_tag="PL162_07020"
                     /EC_number="2.7.7.81"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid cytidylyltransferase"
                     /protein_id="HQF91804.1"
                     /translation="MDSNLAVIPARGGSKRIPRKNVRDLGGRPMIAYTIVAALESGVF
                     DRVVVSTEDEGIAEIARSLGAEVPFRRGEDLADDVTPVSQVTLDALRRLDPEGTRHTR
                     VCQLMANCPVRDAADVRASLEQFKASGAGSQLSVTRFAWQNAWWAMRRHETLALEPLF
                     PREIKMRSQDLPPLFCPTGALWWARSEVLREFGTFYAPGVTGWEISWQHATDIDTEED
                     WAMAELLLQMERQGRGGQK"
     gene            complement(8214..9608)
                     /locus_tag="PL162_07025"
     CDS             complement(8214..9608)
                     /locus_tag="PL162_07025"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HQF91805.1"
                     /translation="MRTESGFDALSIRRIREGYEMGVLTPRDVAELCIARTEALEERY
                     GAWVSFDPEKLRAQARASAVRLEAGLPCRPLEGIPVGVKDIFNTVDFPTEMGSPLWKG
                     FTPGNDARAVFHLRRAGAVLPGKTVTAEFAVHALLGATKNPHDMLRTPGTSSSGSAVA
                     VALGMVPAALGTQTAGSLVRPASYCGVYCCKPSFGVVPRTGILKTTDTLDTVGFFVGH
                     EEDLAPMFEALRVQGPNYPYVFRALEDRRRQGRPEGRPWRVALVHTATWNQAAPHARE
                     ALEGWGRRAESAANVVVEEAELPAVLAEGHAVHRVLYHRCLAYYFREEAKERLRVSPL
                     MRDILDDGARISLEEYRRAEARQRELQQSMEEFFSRYDALVSLATAEEAPLRGEAERD
                     DPALLWTLAHLPVVGAPALRSPRGLPLGLQVVARKYHDRQLLTLCADLTEAELLPSRM
                     VRAEEVFGERGEVR"
     gene            complement(9598..10824)
                     /locus_tag="PL162_07030"
     CDS             complement(9598..10824)
                     /locus_tag="PL162_07030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl sugar amidotransferase"
                     /protein_id="HQF91806.1"
                     /translation="MGEKFGKPLLGDRLQYCTRCGMPETVEKIAFDDMGICRACQSAE
                     QKMHIDWAERERQLEALLQWGRDRARGGYECVVPISGGKDSTFQLHVLAKVHGMKCLA
                     VTFNHNWYSETGWYNLVNCLEELDVDHVMFTPRRSLVNRLARRSLEKIGDACWHCHAG
                     CGAFPLQVAVKFHIPLIAYGESVSESSGRSSYYEPTMPYDREYFTRVSAKATPEEMAC
                     SYIAEDELCPFWLPSPEEMEAAELKGFHLGDYIFWDEERQMEFVRDTYGWKETDIEGA
                     FKGFKSAECVMPGVHDFTCYLKRGYGRSSWQAFTDVRAGLLTREEGFELGKSIDPQRP
                     EVLDYYLQITGYSEEEFREIMARHRVPPLQDVELPVLPKDHPCRERLLPFARQLVEKH
                     RTTHPETERGSSSHED"
     gene            complement(10831..11400)
                     /locus_tag="PL162_07035"
     CDS             complement(10831..11400)
                     /locus_tag="PL162_07035"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HQF91807.1"
                     /translation="MTAPFLEGKRLYLRGLSLEDAEGAYPEWLNDEEVCRGNGHHLFP
                     YGREQAREFIRGTWNDRSAVTLAMVLREGHRHIGNVALQGIHQIHRTAEFAILLGDRE
                     SWGQGYGREAGLLLCRHGFRALQLHRVGCGTFADNEGMRGLARALGMKEEGRRRQAVW
                     KDGRYVDVVEFGVLAEEFFEQFGHQGPKE"
     gene            complement(11397..>12104)
                     /locus_tag="PL162_07040"
     CDS             complement(11397..>12104)
                     /locus_tag="PL162_07040"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQF91808.1"
                     /translation="SLNFHKHIHCGEGGVVVTDDDDLALRVRMIRNHAESSLDNSGIT
                     DLRNMVGFNFRMTELEAAVARCQLRKLDHVLPERQENCAYLNARLGEIPAISPTWVRE
                     GATHSYYFHALTFDEGVAGVSRNRFIDAVRAELPVTELREKEGVNLRAGYVKPVYLLP
                     MFQERQAYGSRHCPFVAPWHEGNVSYAPGICPVCERLQSSEIFVHELMRPPFRREDLD
                     DVVRAFEKVWEHRKDLA"
BASE COUNT         2200 a         4230 c         3446 g         2028 t
ORIGIN      
        1 gttccgtgag gagggagccg caggactcgt tcacaaggga cggggcaagc cttcggggcg
       61 aagatttgcg caggaaattc gggatttcgt ggcagagaaa gcgaggagtg tttgttgcaa
      121 aatacagttc gagcaaacct cccagctgct tcaggaaggc tatccgcttg ttgtccgaac
      181 ggccgctgtc gatcatccgc ttagccgcca gcagtgccag gtcgaagggg ttggggctct
      241 tttccagtcg atcggcgtcg agctcccaca gttttaccgt cggatagacg aagcgaagtt
      301 cattgccaaa ggaggcgtgc tcgtaatgcc cggcctcctt ccccggcctc ttggcggtga
      361 gaagggccag cgatacgacg tcgacgactc ccttgcgaac ctttgccgtc ttccgtcctc
      421 cgtccccggc ggcgtcgacg agctcctcct cgtacttttt gcgcttcagg tatttgaagc
      481 ggattatgga gtggtggtag aacatgcgct cggggaagtt ctcccctccc ttgtcctgca
      541 cctcgacgtg gagggcaagc cagatgtcgc ggcctccctt caggggcacc cgggccagaa
      601 gatcgggggc ctgccaggcg ccgcccataa gggccttgag gtctttcatc tccttgtcta
      661 gaaattcggt ctcgcgccgg gggtcgacct gggcggccag ctcgggcagt atcgatgcga
      721 gcatctcgtc gaagttgact tctgtgatgg tcttccacat ggagtcgccg tcggcgcgct
      781 cgtcgtcttc cgggcaggct tgtcctccgc gcatcgctct tcggcgacgg acgtcgggaa
      841 gagttcttcc cccgggcggg ccttctggat cacgggagtt tctttggccg tctggtccat
      901 ggcttctcct tccgcattgt tcggatggta cagcctcttc cccaagggag tccgaggagg
      961 gcgccttagg gcgcgcctct tcggtggctc ttgaggaagt gaaactccaa gtctccgatc
     1021 aggcgatggc cgtcttcctg gcgccggagg gtaaagacgc tgtggtggcc ttcccccgtc
     1081 gcccggctcc ggggcgatct tctgatcttc tatggtggca ttttaccgca aacaaagggc
     1141 ccgtgggagt aaaaggagcg tggaagggga aacattttgc tcaaaggttt gactctgtcg
     1201 cctctctggg cgaagggcgg agtcgggtcg gcgggaaggg ggtgctaccg acggcagtag
     1261 actaaagaaa gccggctgga agatgcgcgc tcccggccat ggcgctcccg gctacgcgat
     1321 cccggcaccg gaggcgcctt tcgtgcggcg gaaggcagac ggtgctggtt ttcgcgacgc
     1381 caaccttgga ccagaccctt cggcgcggca ggccccgctg ggggggcccg gaggctactc
     1441 cggggttttt acgttccctc accgcactcc ggcgagaagg gcttcctcca gggtcttccg
     1501 gcgctcttcc catcgggggc gcagcatggc catccagagg agatcccccc gctctcctcc
     1561 tccgagggac acatgacgcc gccagcatcc ttccagttca aaccccgccc gaaggtacat
     1621 gacgagggcc tgggggttga ccgccagcac cgacgtgaag aggcggaaaa gccccgattc
     1681 gtcgaaggcc cagcgaagaa tctcgaaaag aagggcgttg cccagacccc tgccccgaaa
     1741 gcgcggatct ccgatgtaca tgccccaatc gcttaccccc gccgcgaggt ccaggtcctt
     1801 cagggtgacc acgccaaagg gctcgtgatc cacgaaggcc acccgaaccc gctggcgatg
     1861 aggatgccgc cgcaggaatt ccagccattg ggcatgctcc tcgggggaaa tggggtgctg
     1921 gcgcaccatg gaagaacgca cttccggcgt gttacgccag aggcgcacct gctcccccat
     1981 ctccggagaa agctccgtca gatcgcggta cgagagagaa atcatgcctc atcccctcct
     2041 cagatgaccc cgggaccgcc ggagtcgccc ccgcaccgac cgtgtagggc gttcctgctc
     2101 ctccaatttt tccaaagcaa ggccccttcg gtcacggtac gtgagagaaa aactcgctca
     2161 caccatctcc caggtgatcc actggtccgc cgccacgtcc tgccgcgcca ctcgccccag
     2221 cagcagggga aggagcttgg gccggatgcc gtaccccgga cgcttcacgc agagcatctc
     2281 ttccgtgatc gccgttccgg cggcaatgtc caccgcggcg tgcacgctcc gccgggcctt
     2341 ttcgtagaac tcccgctcct cgggggccgg tccccgcttt cggccgtccc ccagggcgga
     2401 ttccacctcc cgcacctggc gcaccagctc tttcagctcc ggcggctcta tggcgaaggg
     2461 atgatcgggg cccggcagtg tgcggtccag ggtaaagtgc ttttcgatca ccgacgcccc
     2521 cagggcggcg gcggccacgc tcacgtggat cccccgggtg tggtccgaca accccgtggg
     2581 gaccccgaag gcccgccgca gggtcnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnggggt ctcaatctcc ttgatgaggg agaagagatc ctggtcgtac cccgaatggc
     2761 gaggcgtccg ccgggagtag agcgtctccg cgctgtagat ctggagcttc accgcgtccg
     2821 cccgggcctc caccgcagcg tcgatgagct ccttggccat ggtcagagaa cggttgtggt
     2881 tcgccccgat ctcggcgacg atgaagcacg gggcgccgtc cccgactctt tttcccccac
     2941 gcagcgtcag catggcctca tcctctttcc ctctccgcaa ggcctccacg ccctctctct
     3001 tacgtttttt ccgtctctgc gggagcgacg tcttcggcca ggggccatcg ccggggatcg
     3061 gccaaccccg gaggcatgat ccgccccaga agctccagac gctcctccag atcctccggc
     3121 gcgtttctcg ccccggcggc gctccacagc gccccccatt ctccgatctg gcggggatct
     3181 tcggccccca ccaccacgcc ttcaaggcag aaaagcgtcc ccacggcgag aagggccagc
     3241 tccttgaggg acatgcgaac atcctccgcc aggcttgcca gagccctcct gaactcctcc
     3301 cgccgaagga cgtctccccc cacccgttgc cacggctccc ggtccccctg ggccaggagc
     3361 ccctggagaa aaaggcttcg cccccacaag ggcaccggag gagggctctc ttctccgcgg
     3421 cgctccaact cccggaggga aaaacggcga tccagcagat ttgacggcac ctggagcaga
     3481 tcggggcggg gagtgctgcg caccaaggca tggtactccc cctcatcata aagggacacc
     3541 cccacccgtt ccaccacgcc gccgcgccgc cattacccca gatcccgggc cagcgtcggg
     3601 gtccactcct ccccccgatg cacgaagagc accgccactc ccccggtgcc cagggcctcc
     3661 agagagctct ccagcgaccg aaggcactct ccgggagcaa gccccgcccc gggggcgagt
     3721 ttggtggcca cccgggcatc ccgccgctcg gcgtgagaaa gggagcggag gaacgccccc
     3781 agacgccgct cgctttcccc ataggcccgg gcggtgtcga accagcgcac tcccgcgttc
     3841 caggccgctt ccagcacttc ccgcccctga acgtccgaaa gcatgccccg gtggttggcg
     3901 gcgccatagg ccatgcccaa ttgggccgta cccaaaatca gcgcgggggt tttcgtcgcc
     3961 ccccgcatcg ctccatcggt tttcacagat cttttcccga agcctgggag ctcggagccc
     4021 ccatccacgc cgccacgcgc cggataccct cgtccagcga gatccgggga gcccatccca
     4081 gacgatcccg ggcttttcgg gcatcgcacc gcaacaccgc aatttcgctc tgggggtgaa
     4141 tgtggggcac gtggccgatc tccgcggctc cccctcccac ctcccgggag acgagggccg
     4201 ccaggtcgtt gatggacaca tcccgccccg tgcccgcgtt ccagatctgc cccaggacct
     4261 cggggcactc tccggcgcgg agaatgaaat ccacacaatc ttctacaaag agcaaatccc
     4321 gggtttgggt gccgtcgccg tagatggaca aggatttccc ctccagggcg ttccggacga
     4381 aaatggccac cactcccccc tctccgccgg ttttctggcg gggaccgtag gtgttgaagg
     4441 gacgcagcac caccgtggga agcccgtacc cgaagtggta ggacagtgtc agggcctctc
     4501 ccgccagctt ggcggcggcg tagggagaag cgggcttcac gggggatttc tctccgatgc
     4561 ccggggcgtc ctcgcaccga tcatagacca tgcaggtgga catgaagaca aagcggcaag
     4621 gggtggcacg cacccgctcc agcaggtgaa acgttcccac cacatctcgg tcaaaggtct
     4681 ccgccgggtc atccagactc ttctggacgt tgatctccgc cgccagatga tacaccacgg
     4741 aaaaaggccc ccggcgccac acctcgtccc gcacggcggc ggcacacacg ttcccccgca
     4801 ccagggaaaa gtttccgttt tccgccagat gggccacgtt ttccggacgg ccgttgctga
     4861 agtcgtccac cgccaccacc cgttcccccc gggccagaag ggcatccacc agatggctcc
     4921 cgatgaaccc cgcaccgcca gtgaccagac atgtcatgat tttttcctcc gtttttcggg
     4981 catccacacc ggacgccaga agtcgccccc ccgggagcct ccgcactgcc tcaaattttt
     5041 gcccccggac ggtcggcgca agaaacgccc tccacgttac aaagaccgtc cccggggttc
     5101 ctccatatcg ggcaaccgcc ccagggcgct ccacatctct tccaccaggc acgccgtttt
     5161 ctccaggggc aacaccttct ggccatcgct ccggtactcg ccgggagaag ccacccaggc
     5221 tccgggatag cgcccaaccc gggcatcggg atttttcccg aagggcagga tgaggaacat
     5281 ggactccgtt tccagagccc ggtgagattc ttcctcagtc atcagttctt caaacatctt
     5341 ctctccaggg cgcagaccga tctgatccat ccgaaaggga tctcccggac gttcccgacg
     5401 ccacagcacc tccgccagat ctcccagacg caccacgggc atcttgaaca caaaggtttc
     5461 gccccccacg ctcagatcca tggcctggag catgagctct gtggtccggt ccacggacat
     5521 gacaaagcgc gacatctccg gatgggtcac cggcagcgcc cgcccctcnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnntg tttcagcgcc gccagatgga acaccgcctc
     5701 cacaccctcc atggcccgca gcagacggtc ccggtcccgc acatcgccca gcagaaaacg
     5761 acagcgcccc tccgtactcc aggcgttggc cagcagatac tgcttgtatt catcccggga
     5821 aaagatgcgt accgtttcgg ctccctgggc caacagttcc tgcaccaacc ggatgcccac
     5881 cgtgcccgtc ccaccgatga caagagcttt acggattctc accgcgcccc gcccccttct
     5941 ctcccacacc cctgcccccg agctcctgta ccagaagacg ccggaggaac acctcgccgc
     6001 acctcaccga gtgtcccacc cgccgtccac gggaagcacg gtgcccgtga cataagaagc
     6061 ttcggaggag agcagaaacg ccacggcccc ccccatctcc tccagatttc ccaggcgccc
     6121 cagaggggtg cgggcgccgt aggcttccac aaaagccacc gactgcctct cggggttgcg
     6181 cacccccccc ggagccaccg cattcacccg gatgttccag ggcccccaga gccgggcaag
     6241 acaccgcacc agatgctcca gggcagcctt ggccgccgca tagggaagtc ccgcgaaaag
     6301 gccattttcc atcataaaat catcgtaaat tcccggatcc accgcatggt gcccgtagat
     6361 ggagctcacc gccaccacgg ctcccggaag cgttctctcg tgccagtaca gggcacactg
     6421 ccgcgccact tcaaagaacc gaacggaaga aagaaggtac tcttcctccc aatcggcgga
     6481 acacagcgac cgccactcca cctgaggctt ccgggacgcc accgtatgca ccaaagctcc
     6541 cagaggagga gcgcccacgg catccatcgt tctccgcagt tccccgaacc cctcccggac
     6601 gtcctcttcc gtttcccacc gccagagcac ggggtggacg ggccttgtgc cggcgcgttc
     6661 ccggaaggcc tgggccgaaa cctcgtcccg ggaggtgggg aacaccgtcc accccttctc
     6721 cgccagatgg aggctcaggg agcctccgag ccctccgcac cctccggtca ccagcaccgt
     6781 cctctcaccg gaaggcatgg gcccccccct cctccaccag caggcagcac agggaaaaat
     6841 ccagagcgga gcggatatgg atcgaccgcg cctcgggggt ctccagcatc cgcaacggat
     6901 cgacgaaaaa atgccgccgg gatgccacca cctcccgacg ccccaccttg atcatgccgt
     6961 tggggcgaaa cacgggggga agaagggatc ggtccttcag aaacccatcg ccaaaccaca
     7021 tgccgtacgt cccgttttct tcggggcgca gcacccagtg gaaaaaatgg ggatccaccg
     7081 gcgttcccga caccacacac gccgtatcgg gaaattggac aaaaagatcc cgggcccccg
     7141 taagatcttc gggaaggcga aagggagacg tgggctggag gcaccagagc aaatcctccc
     7201 ctgttccctc ccgttcccag acgtacagca gggcgtccgt ggaagacacc gcatcccccg
     7261 tcacctcgag gggaagcaga aaaggcaccg ccgcccccgc ctcccgggcc agatcggcgt
     7321 agtggggatc gtccgtggcc acgaaaattt cccggaagag accggatttc cgagcggcct
     7381 caatgctgta ggccaacacg gggtgccccc ccagttccag aatgttcttg tgcgccagcc
     7441 ccttggagcc actccgggcg ggaacaacgc caaaatggcg ccccggagcc ccggcggtca
     7501 tttctgcccc ccccggcctt ggcgttccat ctgaagcagg agctccgcca tggcccagtc
     7561 ttcctcggtg tcgatgtccg tggcgtgctg ccaggagatt tcccaacctg tgactcccgg
     7621 agcatagaac gtgccaaact cccggagcac ctccgaccgg gcccaccaca gggctcccgt
     7681 ggggcaaaag agcgggggca ggtcctgact ccgcatcttg atctcccggg gaaagagggg
     7741 ctccagcgcc agggtttcat ggcgtcgcat ggcccaccag gcgttctgcc aggcaaagcg
     7801 ggtgacagaa agctggctcc ccgctccgga ggccttaaac tgttccaggc tcgcccggac
     7861 atccgccgcg tcccgcaccg ggcaattggc catgagctga cagacccggg tgtggcgggt
     7921 gccctcgggg tcaaggcggc gcagggcgtc cagcgtcacc tgggacacgg gcgtcacgtc
     7981 gtccgccaga tcctctcccc ggcgaaaggg cacctccgcc cccagcgaac gggcaatctc
     8041 ggcaatccct tcgtcctcgg tgctcaccac cacccggtcg aaaacgccgc tttccagggc
     8101 ggccacgatg gtgtaggcga tcatggggcg cccccccaga tctcgaacat ttttccgggg
     8161 aatacgtttg gatccccccc gggcgggaat caccgccagg ttgctgtcca cggtcaccga
     8221 acctcccctc gttctccaaa gacttcttcc gcccgaacca tccgggaggg caaaagttcc
     8281 gcctccgtga ggtccgcgca cagcgtgagc agttgccgat cgtggtactt gcgggccacc
     8341 acctggagcc ccaggggaag cccccgggga gaccggaggg cgggggctcc caccaccggc
     8401 agatgggcca gggtccacag cagcgcgggg tcgtcccgtt ccgcctctcc ccgcaggggg
     8461 gcctcttccg ctgtggccag cgagaccaga gcgtcataac gcgagaaaaa ctcttccatg
     8521 ctttgctgaa gctcccgttg acgcgcctcc gcccgccggt actcctccag agaaattcgg
     8581 gcgccgtcgt cgagaatatc ccgcatgaga ggagaaaccc gtaaacgctc cttggcctcc
     8641 tcccggaagt agtaggcaag acaccggtga tagagtaccc gatgcaccgc atgcccctcc
     8701 gccaggaccg ccggaagctc cgcttcctcc accacgacat tggccgcact ctcagcccgc
     8761 cgtccccagc cctccagagc ttcccgggca tggggagcgg cctggttcca cgtggcggtg
     8821 tgcaccagcg ccacacgcca cggccgcccc tcgggacgcc cctgtcggcg ccgatcctcg
     8881 agagcccgga aaacgtacgg ataattcggc ccttgcaccc gcaacgcttc gaacatcggc
     8941 gccagatctt cctcgtgccc cacaaaaaaa cccaccgtgt ccagggtgtc tgtggtcttc
     9001 aaaatccccg tccggggcac cacgccgaag gaaggcttgc aacagtaaac cccgcagtaa
     9061 ctcgcaggac gcaccagaga tcccgcggtc tgggttccca gagcggcggg caccatgccc
     9121 agagccaccg ccaccgccga accgctggag gatgtccccg gcgtccggag catgtcgtgg
     9181 ggatttttcg tggccccgag cagggcatgg acggcgaact ccgccgtcac ggtcttcccg
     9241 ggaagcaccg cccccgcccg gcgaagatga aacaccgccc gggcatcgtt gcccggggta
     9301 aagcccttcc agagggggct tcccatttcc gtgggaaaat ccaccgtgtt gaagatgtcc
     9361 tttaccccca caggaatccc ttccaggggc cgacaaggga gccccgcttc cagacgcacc
     9421 gccgatgccc gggcctgggc ccggagtttt tccggatcaa aggagaccca ggctccgtaa
     9481 cgttcctcca gggcctccgt gcgggcaata caaagctccg ccacatcccg gggagttaaa
     9541 acgcccattt catacccctc tcgtatccgg cggatggaca gggcgtcaaa acccgattca
     9601 gtcctcatgc gaagagctcc ccctttccgt ttcgggatgg gtcgtcctgt gcttctccac
     9661 cagttgccgg gcgaagggga gcagacgctc ccggcaggga tggtccttgg ggagcacggg
     9721 gagctccacg tcctggaggg gaggcacccg atgccgggcc atgatctccc ggaactcctc
     9781 ctcggagtag ccggtgatct gaagatagta atccaagact tccgggcgct gggggtcaat
     9841 ggattttccc agctcaaacc cttcttcccg cgtcaggagc cccgcccgca cgtcggtgaa
     9901 cgcctgccag gaggagcgcc cataccctcg tttcagatag caggtgaaat catgaacccc
     9961 gggcatgaca cactcggcgc tcttaaatcc cttgaacgct ccctcgatgt ccgtctcctt
    10021 ccagccgtag gtgtcccgga caaactccat ctgacgttcc tcgtcccaga aaatgtagtc
    10081 ccccagatga aaccccttga gctccgccgc ctccatttct tcgggagacg ggagccagaa
    10141 gggacagagc tcgtcttcgg cgatgtagga gcaggccatt tcctccggcg tggccttggc
    10201 ggagacccgg gtgaagtatt cccgatcata gggcatggtg ggctcgtagt acgaagaccg
    10261 ccccgaggac tcggacaccg attctccgta cgcgatgagg ggaatgtgga acttcaccgc
    10321 cacctgaagc ggaaaggccc cgcatcccgc gtggcagtgc cagcaggcgt ccccgatctt
    10381 ttccagcgaa cgccgggcca ggcggttcac caggcttcgg cgcggagtga acatgacatg
    10441 gtccacatcg agctcttcca gacaattcac gaggttgtac caccccgttt cgctgtacca
    10501 gttgtggttg aaggtcaccg ccaggcattt catgccgtgg accttggcca acacgtgcaa
    10561 ctggaaggtg ctgtccttgc ccccgctgat gggcacgacg cactcgtacc ctccccgggc
    10621 ccggtcccgc ccccactgga gcaaggcctc cagctggcgt tcccgctccg cccagtcgat
    10681 gtgcatcttt tgctccgccg actgacaggc ccggcagatc cccatgtcgt caaaggcgat
    10741 cttttccacg gtttccggca tgccgcaccg ggtacagtac tgcagtcggt cccccagaag
    10801 cggtttcccg aacttttccc ccataacccc tcactccttc gggccctggt ggccaaactg
    10861 ctcaaaaaac tcctccgcca gcacgccgaa ctccaccaca tcgacgtacc gcccatcctt
    10921 ccaaacggcc tggcggcgtc gcccctcttc tttcatccct aaagccctgg ccaacccccg
    10981 catcccttcg ttgtccgcaa aggtgccgca tcccacccgg tgcaactgga gagcccgaaa
    11041 tccgtggcgg cagagcaaca accccgcttc ccgcccgtag ccctggcccc agctctcccg
    11101 gtctcccaga aggatggcga actccgccgt gcggtggatc tggtggatgc cctgcaacgc
    11161 cacattcccg atgtgccgat gcccctcccg gagcaccatg gccagcgtca ctgcggagcg
    11221 gtcgttccag gtgccccgga taaactcccg ggcctgctcc cggccatagg gaaagagatg
    11281 gtgcccgttg ccccggcaga cctcttcgtc gttgagccac tccggatagg ccccctccgc
    11341 gtcctccagg gacaaccccc ggagatagag ccgctttcct tcgagaaaag gcgccgtcat
    11401 gcgaggtcct tccggtgctc ccagaccttc tcaaaggccc gcaccacgtc gtccaggtcc
    11461 tcccggcgaa aaggagggcg catgagctcg tggacgaaga tctccgaaga ctgaagccgc
    11521 tcgcacacgg gacagatccc cggggcgtag gacacgttcc cctcgtgcca gggggcgacg
    11581 aagggacagt gcctggaacc ataggcctgg cgctcctgga acatgggcaa aaggtacacc
    11641 ggtttgacat accccgcccg gaggttcacc ccctctttct cccgcagttc cgtcaccggg
    11701 agctccgccc gcacggcatc gatgaaccga tttcgagaca cccccgccac cccctcgtcg
    11761 aaggtcaggg cgtggaaata gtagctgtgg gtggccccct cccggaccca ggtgggagaa
    11821 atggcgggga tttctcccag acgggcgttc agataggcac agttttcctg acgctcggga
    11881 agtacatgat ccagttttcg gagctgacac cgggccaccg cggcctccag ttccgtcata
    11941 cgaaaattaa aaccaaccat gtttcgcagg tccgtgatcc ccgaattgtc gagagaagac
    12001 tccgcgtggt tgcggatcat gcgtacccgc agggccaggt cgtcatcgtc ggtgaccacc
    12061 acgcccccct cgccgcagtg gatgtgcttg tgaaagttga ggga
//
LOCUS       DAOOER010000049        12073 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_5829_length_12073_cov_8.260359, whole genome shotgun sequence.
ACCESSION   DAOOER010000049 DAOOER010000000
VERSION     DAOOER010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 12073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12073
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5829_length_12073_cov_8.260359"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /gene="whiA"
                     /locus_tag="PL162_07045"
     CDS             <1..626
                     /gene="whiA"
                     /locus_tag="PL162_07045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HQF91809.1"
                     /translation="ECIESHRMSWPWVRGLWGAAGALYLPKNGYFLVLRLSVQNPLLP
                     EVEAFLEDEGCPGSKRLHEGKEELLLRHQQSIAIFLSRMGLCGTSLRLEEHALFRSMR
                     DQANRLVNCDSANIKKSLLAAERQLEECRQVVAYGLMEILPRELQELVRVRLQHGSAS
                     LQELGQLVQRPVSKSTVEYRWGKLRRLVGDLLQQRKEGRSCIWENPK"
     gene            602..2563
                     /locus_tag="PL162_07050"
     CDS             602..2563
                     /locus_tag="PL162_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HQF91810.1"
                     /translation="MYLGKSEVNTFEKGAAQEILLTNGRRGYAFSSVIGVNTRQDQGL
                     LVVPLPGSGGHGVLVSKVDETLAIGGRRFLLSTNRYRDMVYPDGFRYIQEYFETPLPS
                     VLFVLHSVFVRKTIFMVPGQPWTVVKYEVLASPERFQLEIRPLMAHRQRNLPGERRSC
                     QSFRSHEMPPQGLCVEGRCMKTFFNATEGEWFLKELWFEGMLYGGDGLGSHDLEDVWS
                     PGYLSLDLGAGETVYLVMGHTPVPGDFAVLRRMEKDAEASFERPFPRALGAWSESAKG
                     LVRSAASLVSLGGPEGEPAIFSGYPSLRDLARDTFVALPGLTLATGRTGVAGAILDRW
                     LRLAERHDGVMPGEVLSDGATLLGPADGGLWFFYALDKYCQYVGSFDKISLHWQNLTK
                     LLGRYEEGIPALDLVCGENGLLHLTTVNPERHWMSGEVEGVPVVPRRGALVEINALWY
                     NALRVMERFADIVEDRRSARRFGEGADAVRESFTKTFFNTAQGSLFDWVDGDERDDDI
                     RPNQLLAVSLPCSPLEPEIGRSVLEVCWNGLYTTYGLRTLDAHSGKYKGRCEGRPDQR
                     RKAWYRGMAWPWLLGQFVSAFLRYNPHQRDLGALFLRPFLHRPGGLGGVAELFDGSMP
                     YDPHGDAVSAMSVGEILRVMEEDLRPLGL"
     gene            2671..3690
                     /gene="gap"
                     /locus_tag="PL162_07055"
     CDS             2671..3690
                     /gene="gap"
                     /locus_tag="PL162_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HQF91811.1"
                     /translation="MGERVKVGINGFGRIGRLVLRSLLLAPEKHLFDVVAVNDLASPE
                     SLAYLFKYDSVYRRFPGEVRADGGAVVVDGRRVEVLAEKDPANLPWKKLGVDVVVEST
                     GRFTDGEKAKAHLDAGAKKVIISAPASNEDTTIVIGVNDDMYDPAKHRIISNASCTTN
                     CLAPVAKVLQDNFGIVRGLMTTTHAYTNDQTILDFPHKSKSRGRAAALSIVPSTTGAA
                     KAIGKVLPELKGKMNGIALRVPTPVVSMVDLVAELKCSVTADQVNAAMKAAAEGPMKG
                     ILAYEEEDLVSMDFVGDPHSSLFAPMHTIAIENSVKVFSWYDNEWGYSNRVLDLVDLL
                     IKKGL"
     gene            3694..4887
                     /locus_tag="PL162_07060"
     CDS             3694..4887
                     /locus_tag="PL162_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HQF91812.1"
                     /translation="MKLRLFSPEDVRGKSVLVRVDFNVPMQKGQVTDTTRICAHLATL
                     KALRDGGARVGLVSHLGRPKGTRNMEYSLSPVAKALEKLSGWPVSMVDDCVGPEVSRA
                     LASIPEGALLMLENVRFYPEEERNDAIFAKELAAPFDIFVMDAFSAAHRAHASTRGVA
                     DLLPAFAGELMRKELEALGTVRDHPKHPFVLILGGAKVSDKIGVIENMLGHVDSILVG
                     GGMAFTFLKAQGKEIGKSLCETEKLDFAREMLDRAREKGIDLLLPEDAVVAPELKDGV
                     LGAVVSTDAMPEGQMGLDIGPRTADRFAQALKSAQTVLWNGPMGVFEIPAFVEGSQKI
                     ARAMVEATARGALTVVGGGDSAAAIAQFGLEKGVSHVSTGGGASLEFFEGLPLPGVEP
                     LLDPE"
     gene            5274..6065
                     /gene="tpiA"
                     /locus_tag="PL162_07065"
     CDS             5274..6065
                     /gene="tpiA"
                     /locus_tag="PL162_07065"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HQF91813.1"
                     /translation="MRKILCAGNWKMNLAPAAAKAFGEELRTVLMGEEGAGRRVRAGQ
                     IELALMPPFVSIAPVRQALEGMPVTLGAQNCHEEPQGAFTGEIGAPMLAEMGCHYVII
                     GHSERRHIFGETDELVMKKIKAVKDSGMVPILCVGELLEEREGNRTLAVLERQIRAVL
                     PQMAPEDVRDHFVVAYEPVWAIGTGKVASEDDAQEACRFIRELAATCCGAEAAEALRI
                     LYGGSVKPENAAGLVAQQDVDGFLVGGASIKVASLMGIVAASVKK"
     gene            complement(6412..6783)
                     /gene="rsfS"
                     /locus_tag="PL162_07070"
     CDS             complement(6412..6783)
                     /gene="rsfS"
                     /locus_tag="PL162_07070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HQF91814.1"
                     /translation="MDQTLDNFEDVSPLVKALEEKHAEEILALDLRRNTTIADFFVLA
                     TANSTTHLQTLQDAVEEAFERQSISYRREGDESTKWRVVDGGDVVVHLFSRGGRDFYR
                     LERIWGDVPSKRFDSFSQDPS"
     gene            complement(6947..8278)
                     /locus_tag="PL162_07075"
     CDS             complement(6947..8278)
                     /locus_tag="PL162_07075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HQF91815.1"
                     /translation="MNKARFFLLLLVALLAGGAGVAMRLRHVVTPHSGDIRENFQYDT
                     ASGAINILLIGVDETDQSHRADSLAVVRINVDSRHIRIMSVPRDTRVQIPGHGWQKIN
                     HAFAFGGVELVQKTIVNFLGIPLDYFVVVNYDSFPRLVNLVGGVDLFVEKNMKYRDRS
                     GGLYIDIAAGNQHMDGQTALHYVRFRHDALGDIGRVQRQQKFLVALLKRIKEPRMLSR
                     LPELAQEVVGMVDTNLTVSQAVQLAAYLKDLSSGDRLKFSTLPGSPAYIAGISYWLPN
                     LAQASLFFSEEERSGDITSPDRSSPSDLEISGGELSAASTDSSLRSSDTPLMASSKDG
                     DALGKIQRPVAVLNGNGGKGLSHSFSKRLQELGVDVTYVGNAKHFDYHTSNVLYPEGA
                     PREVVQTAKALAEMCGISQKMVKANRKSTYITLIIGHDYKEILKKIGGTYP"
     gene            complement(8283..8924)
                     /gene="nadD"
                     /locus_tag="PL162_07080"
     CDS             complement(8283..8924)
                     /gene="nadD"
                     /locus_tag="PL162_07080"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HQF91816.1"
                     /translation="MVHGAPSPLKGLQRIGIMGGTFDPIHYGHLLAAEEAFSSLGLDR
                     VIFVPTGESPHKRHRQVSTAEDRYAMVLLATLDNFHFSLSRVEIDRGGASHTVDTLRE
                     MRHWFSPSHAEFFFITGMDAVLQLDTWKNPELLPGLCHIVAVGRPGFDASDLLNLSSS
                     LTKSIVFLEIPLLAISSTEIRSRIAAKKSIRYLVPSAVENYIIKKKLYGYAGA"
     gene            complement(8979..10355)
                     /gene="obgE"
                     /locus_tag="PL162_07085"
     CDS             complement(8979..10355)
                     /gene="obgE"
                     /locus_tag="PL162_07085"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HQF91817.1"
                     /translation="MQFIDEAQLSVRAGQGGDGCLSFRREKYIPRGGPDGGNGGHGGS
                     VFIEASPALHTLADFEYQRHYRAEDGDHGRGKNQHGRRGDDCVIHVPCGTVIYDRRSE
                     TPLADLVEPGDRLLVARGGRGGRGNAHFANAVRKAPRFAERGGVGVEVELRCELKLIA
                     DVGLVGMPNAGKSSLLAAMSNAQPKIASYPFTTLSPNLGIVSVDDDRIVLADIPGLIE
                     GAHDNCGLGLAFLRHVERTRLLLYVIDVSSGDPKEWRSQWDILRREFAAYDPSLLERP
                     HMVLGNKIDLTSDRSLLEEGFFEIFRATGTQAFLVSALSGEGVDALLHAVIAFVRHHP
                     RPRGQTRFFSSADSLNEGIPYSLEVVRLPGRDGFRLLHPHLERAVRRFDFNQDEAVHR
                     FNNLLRKYRAEQALAASGAKEGDTVWIGDTGFNFEPERAKSDARPYDSSKKDDREPGD
                     DGEERLSV"
     gene            complement(10724..11011)
                     /gene="rpmA"
                     /locus_tag="PL162_07090"
     CDS             complement(10724..11011)
                     /gene="rpmA"
                     /locus_tag="PL162_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HQF91818.1"
                     /translation="MRVFDLQLLAHKKGQGSSTNGRDSQPKMLGVKRQDGQFVLAGNI
                     LVRQRGTKIRPGLHVGRGRDDTLFALETGLVRFETKAGHKFVRIEVVAAEA"
     gene            complement(11364..11675)
                     /gene="rplU"
                     /locus_tag="PL162_07095"
     CDS             complement(11364..11675)
                     /gene="rplU"
                     /locus_tag="PL162_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HQF91819.1"
                     /translation="MYAIVETGGKQYRVEEGTVLKVERVPFAEGTGFSIDRVLLVAKE
                     EGVTVGSPFVEGVQVRATVLSHGKADKVYIFHYRRKKNYRRFRGHRQPFSEIRIESIA
                     L"
BASE COUNT         2539 a         3301 c         3624 g         2609 t
ORIGIN      
        1 ccgagtgcat tgagagtcat cgcatgagct ggccttgggt tcgaggcctt tggggggcgg
       61 cgggggccct ctacctcccc aaaaacgggt actttctggt gttgcgcctt tccgttcaga
      121 accccctttt gccggaggtg gaagcctttt tggaagacga gggatgtccg ggatcaaaac
      181 gactccacga ggggaaagaa gagctcttgt tgcgccatca gcaatccata gcgatctttc
      241 tctctcgcat ggggctttgt ggaacttctc tgcgccttga ggagcatgcc ctgttccgat
      301 ccatgcggga tcaggccaac cgattggtga actgtgattc cgctaacata aaaaagagcc
      361 tcctggccgc cgagcggcag ttagaggagt gtcggcaagt ggtggcttac ggcctgatgg
      421 agatcctccc gagagagctt caggaactcg tacgggttcg attacagcat gggagtgcct
      481 cgttgcagga attgggccaa cttgtgcaga ggcccgtgag caaaagcacc gtggagtacc
      541 gatggggtaa actgcgtcgt ctggtgggag acctgctaca gcagagaaag gaggggcgtt
      601 catgtatctg ggaaaatccg aagtgaacac ctttgaaaag ggagcggcgc aggaaattct
      661 tttgaccaat ggacgccggg gttacgcctt ttcgtctgtc attggggtaa acacccgtca
      721 agatcaaggt cttctcgtgg tacccctgcc gggaagcggg gggcatggag ttctggtgag
      781 caaggtggac gagactctgg cgatcggagg ccgccgcttt cttctctcca cgaacagata
      841 ccgggacatg gtttatccag atgggtttcg ctacatccag gaatatttcg aaacccccct
      901 tccttccgtg ctttttgtgc tccacagcgt ttttgtcaga aaaaccattt ttatggttcc
      961 cgggcaaccc tggacggtgg tgaaatacga agtcctcgcg agcccggagc gcttccagct
     1021 ggaaatacgc cccctcatgg ctcaccgcca gcgaaacctc ccgggggagc gacgctcttg
     1081 tcaatcgttc cgtagccacg agatgccccc gcaagggctg tgcgtggagg ggcgatgcat
     1141 gaagacattt tttaatgcta ccgaaggaga gtggttcctt aaagaattat ggttcgaggg
     1201 gatgctctac ggtggcgatg gtttgggcag ccacgacctt gaagacgtct ggtctccggg
     1261 ttatttgagt ttagacctgg gggcggggga aacggtctat ctcgtcatgg gtcatacccc
     1321 cgtgccggga gactttgcgg tgctccgtcg catggaaaaa gacgccgaag cctcatttga
     1381 gcgtcctttc cctcgggcct tgggggcctg gtcggaatcg gcaaaggggc tggtgcggtc
     1441 ggcggcttct ctagtttctc tcggaggccc cgagggggag cccgccatct tttccgggta
     1501 cccatcgctc cgggatctgg cgcgagacac ttttgtggcc cttccgggat tgactttggc
     1561 cacagggcgc acgggagtgg cgggggccat ccttgataga tggcttcgcc tggcggaaag
     1621 gcacgacggg gtcatgcctg gagaggttct ctccgacgga gccacgctcc tgggacccgc
     1681 cgatgggggg ctttggttct tttacgccct ggacaagtac tgccagtacg tgggatcctt
     1741 tgacaagata tcgctccact ggcaaaacct caccaagctg ctgggccgat atgaagaagg
     1801 catccccgca ttggacttgg tttgtggcga aaacggcctt ctgcatctga ccactgtcaa
     1861 tcccgagcgt cactggatgt ccggcgaggt ggaaggggta ccggtggtcc ctcggcgagg
     1921 ggcgctggtc gaaataaacg ccctttggta caatgccctt agggtgatgg agcgctttgc
     1981 cgacatcgtc gaggatcgga gaagcgcccg gcggtttgga gagggggccg atgcggtacg
     2041 ggaaagtttt acgaagacct tttttaacac cgcccagggt tccctgttcg actgggtcga
     2101 cggggatgaa agggatgatg atatccgacc gaaccagctt ctggcggttt cccttccatg
     2161 ctcccccctg gagcccgaaa taggccgttc tgttttggag gtttgctgga acgggctgta
     2221 caccacttac gggttgcgca ccctcgacgc ccattccggc aagtacaagg gacgatgtga
     2281 ggggcgtccc gatcagcgcc gtaaggcgtg gtatcgggga atggcttggc cttggcttct
     2341 ggggcagttc gtaagcgctt ttttgcgcta taatccccat cagagggacc tgggggccct
     2401 attcctccgt cccttcctgc accgtccggg cggactggga ggcgtggcag agctctttga
     2461 tgggagcatg ccctacgacc ctcacggaga tgccgttagt gccatgagcg tgggggagat
     2521 cctcagggtg atggaagagg acctgcgtcc ccttggtctg tgagagcgga gggagcatgg
     2581 tgctccgagc cgccgttttc caaaggtata cccggcgtgc ggggtgccgg agggatgatg
     2641 atcctaaagg acaggagagg agaatgtacc atgggcgaaa gagttaaggt gggaattaac
     2701 ggttttggtc gtataggtcg gctggttctt cggtcgctgt tgcttgcccc cgaaaagcat
     2761 ctctttgacg tggtggcggt caacgatttg gctagcccag agagcctcgc gtatcttttc
     2821 aagtacgact ccgtataccg gagattccct ggggaagtcc gcgccgacgg aggagctgtg
     2881 gtcgtcgatg gccgccgggt ggaggtattg gcggaaaagg acccggcgaa cctcccctgg
     2941 aaaaaacttg gagtggacgt ggtggtggag tccacggggc gtttcacgga tggcgaaaag
     3001 gccaaggccc atctggatgc gggagccaag aaagtgatca tctccgctcc ggcctccaac
     3061 gaggacacca ccatcgtcat cggcgtgaac gacgacatgt acgaccccgc caaacatcgc
     3121 atcatctcca acgcctcttg caccacgaac tgcctggcgc cggtggccaa ggtgcttcag
     3181 gataacttcg gcattgtccg ggggctcatg accaccaccc acgcctatac caatgaccag
     3241 accatcttgg actttcccca taagagcaag agccggggga gggcggcggc cctttccatc
     3301 gtccccagca ccacgggggc ggccaaggcc ataggtaagg ttctgccgga attgaaaggg
     3361 aagatgaacg gcatcgccct gagggttccc acgccggtgg tatccatggt ggacctggtg
     3421 gccgagctga agtgctcggt gacggcggat caggtgaacg ccgccatgaa agcggcggct
     3481 gaaggaccca tgaagggaat tttggcctac gaggaagagg acctcgtgtc catggacttc
     3541 gtgggggatc cccattcgtc gctttttgcc cccatgcata ccattgccat cgaaaactct
     3601 gtcaaggtct tctcgtggta cgacaacgaa tgggggtaca gcaaccgggt tctggacctg
     3661 gtggatctgt tgatcaagaa ggggctctag gacatgaagt tgcgcctttt tagccccgaa
     3721 gacgtgcgtg gcaagagcgt cctggtgcgg gtggacttca acgtccccat gcaaaagggg
     3781 caggtaacgg acactactcg gatttgtgct cacctggcca cccttaaggc ccttagagac
     3841 ggaggcgccc gggtggggct ggtttcccac ctaggccggc ccaagggaac tcgtaacatg
     3901 gagtattcgt tgtctcctgt ggcgaaagcc ctggagaagc tgtcggggtg gcccgtgtcc
     3961 atggtggacg actgcgtagg gccggaagta tctcgagccc tggcgtcgat ccccgagggg
     4021 gccctgctga tgctcgagaa cgtccgattt tacccggagg aagagagaaa cgacgcgatc
     4081 ttcgcgaaag aattggcggc tccctttgac atcttcgtca tggatgcctt cagcgcggcc
     4141 catcgggccc acgcctccac ccggggcgtg gcggacctct tgccagcctt cgcgggcgaa
     4201 ctcatgagga aagagctcga ggccctgggg acggtgcggg accatcccaa acaccccttt
     4261 gtgctcatcc tcggtggagc caaggtgtcg gacaagatcg gcgtcataga aaatatgctg
     4321 ggacacgtgg actccatctt ggtggggggg gggatggcct tcaccttcct gaaagcccag
     4381 ggaaaagaga tcggcaaatc cctctgcgag acggaaaaac tcgattttgc ccgggaaatg
     4441 ctcgaccggg cccgggaaaa aggcatcgac ctcctgcttc cggaagacgc cgtggtggcc
     4501 ccggagctca aagacggcgt tcttggggcg gtggtatcca ccgacgccat gccggagggg
     4561 cagatggggc ttgatatagg cccccgtacg gcggatcgct ttgcccaggc cttgaagagt
     4621 gctcagaccg tcctttggaa cggccccatg ggagtctttg agatcccggc ctttgtcgaa
     4681 ggatcgcaaa aaatagcccg ggccatggtg gaagccacgg cccgaggggc cctcacggtg
     4741 gtgggaggag gagacagcgc ggcggccatc gcccagtttg gcctcgaaaa gggcgtgagc
     4801 catgtctcca ccggaggagg cgcgagcctg gaattttttg aaggactccc tctaccgggg
     4861 gtagaaccgc tcttggatcc ggagtagacc ccatttcttc tcgtcaaccc catgccatgg
     4921 gaggaagagc gatcttcctc ccatatttag tccgtttggc tgcgtagacg agggacgtcg
     4981 cttccggagt tcctttctct ccttccttac aaaacaaagg cgaagacccc gtcggggcgc
     5041 cccgaggggt gggcgccctc tttttcgctt tgttgatggt aagggggcga ctccccacta
     5101 gggggccccg ttgtgcctcc taaggtccca tccgggggct ctattgctga atttaagatg
     5161 tcgtccgggg ggaatcattt ttattttgat aacgatggcg gagagccctc aggcccgggg
     5221 ggaccctgcg ggacgaaaag ccacggccac aacacatgaa aagggtgatt tttatgagaa
     5281 aaatcctttg tgcgggcaac tggaagatga acctggctcc tgcggcagcc aaggctttcg
     5341 gagaagagct gcgtacggtc cttatggggg aggaaggagc ggggcgacgg gtgcgtgcgg
     5401 ggcagataga gctggccctc atgccccctt ttgtgagcat cgccccggtg cgccaggccc
     5461 tggaagggat gccggtcacc ctgggggccc aaaattgcca cgaagaaccc cagggagcct
     5521 ttacggggga gatcggcgct cccatgctcg ccgaaatggg atgccattat gttattatag
     5581 gacatagcga gcggcgccac atcttcgggg agacggatga gcttgttatg aaaaagatca
     5641 aggccgtaaa ggattctggc atggtcccca ttctatgtgt gggggaactt ctcgaggagc
     5701 gcgagggaaa ccggaccctt gcggtgttgg agcgtcagat ccgggccgtc ttgccccaga
     5761 tggctccgga ggatgttcgg gaccattttg tggtggccta tgagcccgta tgggctatcg
     5821 gcacgggcaa agtggccagc gaggacgatg cccaggaggc ctgtcgtttc attcgggagc
     5881 tggcggccac ttgctgcggc gccgaagccg ccgaagccct gcgcatcctc tatggtggca
     5941 gcgtcaaacc ggaaaacgcc gctggcctcg tggcccagca ggacgtggac ggttttctcg
     6001 tgggcggggc ctcgatcaag gtggcgtcct tgatgggcat agtagcggca tctgtaaaaa
     6061 aataacgtca acggtctcgg ggcgccctcc ggatctggtg gttacagggc cgggggcgcc
     6121 cctgtaagca acataagata cattatagga catcgcgcga ccccgacctc ccccacaagg
     6181 gataagacaa ggcggaactc cggaataaga cgggcccgga gaattgggag aatgcctaag
     6241 ggtgctctcg gggacctttg ttgtaaatgt tgtaaaaata tcgagacaga ggccgctacg
     6301 gcgcggcaaa aatcacctga gacatggtag aagttgtccc cgtcgtggcc gtagattttc
     6361 cccttggccg ttgcccgata tcaggcgttt tttatggttt tatcttggga gctacgaagg
     6421 atcttgagag aaagagtcga agcgcttcga gggcacatcg ccccagatac gctccagacg
     6481 gtaaaaatct cgccctcccc ggctgaacag gtgcacgacc acgtctcccc cgtccactac
     6541 ccgccacttg gtgctctcat ccccctctcg gcggtaagag atggattgac gctcaaaggc
     6601 ttcttctacg gcgtcctgaa gcgtctggag gtgggtggtg gagttggcgg tggcgagaac
     6661 gaaaaagtcc gctatggtcg tattccggcg aagatccagc gccaagatct cctcggcgtg
     6721 cttttcttcc agggccttta ccaacggaga tacatcttcg aagttatcca aagtctgatc
     6781 catccttatg tggtctcctt ttctatatct caacaccaga ggttgtgcca gagatcatcc
     6841 gatgatttat gttgtcattc tatattaggc gcgttcctcg gagaatttcc gtccttggtg
     6901 gagcgtggcg cagggtgatg gagaattcct gctgtggcat gatctcttac ggatacgttc
     6961 ctcctatttt ttttaatatt tctttataat cgtgtcctat tatcagcgtg atataggtgc
     7021 tctttctatt tgcttttacc attttttgag aaatgccgca catttcggcc agggctttgg
     7081 cggtctgaac tacttcgcgg ggcgcgccct ctgggtagag aacgttgctc gtgtggtagt
     7141 cgaaatgctt ggcgttccct acgtaagtga cgtcgacgcc cagttcttga agcctcttag
     7201 agaaactatg gctcagccct tttcctccgt tgccgtttaa gaccgccacg gggcgttgga
     7261 tttttccgag cgcatcacca tcttttgatg atgccatcaa aggtgtatcg gaagatcgca
     7321 aagatgagtc tgtggaggcg gcggagagtt cccctccgga gatttccaag tcggaaggag
     7381 acgaccggtc cggagatgtt atgtccccac tgcgctcttc ttcggagaaa aatagtgaag
     7441 cctgggcaag gtttgggagc cagtaactga tgccagcgat gtaagcggga cttccgggaa
     7501 gggttgaaaa tttcagccga tcccctgaag agagatcctt gaggtaagcc gctaactgta
     7561 ccgcctggga gaccgtcaag ttggtatcca ccatccccac gacctcttgg gccagctcgg
     7621 gcaatcggga caacatgcgg ggctccttga tacgttttaa tagggccacg agaaatttct
     7681 gctgtctctg cacgcggcca atatctccca gggcgtcgtg cctgaaccgt acgtaatgta
     7741 gagccgtttg tccgtccatg tgctggtttc ccgccgcgat gtcgatatat aggcctccgg
     7801 agcgatcgcg gtatttcatg tttttttcca cgaaaaggtc cacccctccc accaggttga
     7861 cgaggcgagg gaaggagtca taatttacga cgacaaagta atcgaggggg atgccaagga
     7921 aattgacgat ggtcttctga actagttcca cccctccgaa agcaaaggcg tggtttatct
     7981 tctgccatcc gtggccgggg atctgtacgc gggtgtccct cgggacggac atgatcctga
     8041 tgtggcgaga atcgacgttg atacggacca cggcaaggga atcggcgcga tgggactggt
     8101 cggtctcgtc gacgccgatc agcaaaatgt tgatggcccc cgaagcggtg tcgtactgga
     8161 agttctcgcg aatgtctcca ctgtgagggg tgaccacgtg gcgaaggcgc atggcaaccc
     8221 ccgctcctcc tgcgaggagc gccaccagca gtaaaaggaa aaatcttgcc ttgttcaccc
     8281 catcacgctc ccgcgtatcc atagagtttt ttcttgataa tatagttctc tacggcggat
     8341 ggcaccaaat aacgaatgct ctttttggcg gcaatgcgcg aacgaatttc cgtgctagat
     8401 atggccagga gagggatttc aagaaaaaca atgctttttg tgagtgaaga tgagagattt
     8461 aaaagatcag aggcatcaaa ccccggccgt cccacagcga cgatatggca aagtcccggc
     8521 aagagctcgg ggtttttcca cgtatcgagt tgaaggactg catccattcc tgtgataaag
     8581 aaaaactccg catgagaagg actaaaccaa tggcgcattt cacgcagagt gtccaccgtg
     8641 tggctggctc ccccccggtc aatctccacc cgggaaaggg agaagtggaa attgtcgagc
     8701 gtggccagaa gcaccatggc gtagcggtcc tccgccgtgg aaacctggcg gtgcctcttg
     8761 tggggagact ctcctgtggg aacaaagatg acgcggtcta accccagaga agaaaaggcc
     8821 tcttccgccg ctaaaaggtg accgtaatgg atgggatcga aggtcccccc catgatgccg
     8881 attcgctgca ggcccttgag gggactcgga gctccatgaa ccatgtccac gctcacaagg
     8941 ggacatgtag ttcggaaggg aagagctccg ccgtcccgct atacggaaag tctttcctca
     9001 ccgtcatcgc ccggttctct gtcatctttt ttggaagagt cataagggcg ggcgtcggac
     9061 tttgccctct cgggttcgaa attgaacccc gtgtcgccga tccacacggt atccccttcc
     9121 tttgctcccg acgctgccag ggcttgttcc gctcggtatt tgcgcaaaag gttgttaaag
     9181 cgatggaccg cctcatcctg attgaaatca aagcggcgca cggcccgttc caagtgaggg
     9241 tgcaggaggc ggaacccgtc tcttccggga aggcgcacca cttccaggct gtaagggatg
     9301 ccttcattca aggagtctgc ggaggagaaa aagcgggtct gtccccgggg gcgggggtga
     9361 tggcgtacaa aggcgatgac tgcatggagc agggcgtcga ccccctctcc ggaaagggcg
     9421 ctcacaagaa aggcctgggt cccggtggca cggaatatct caaaaaagcc ctcctccaga
     9481 agagatcgat cggaggtcaa gtcgatcttg tttcccaaga ccatgtgggg ccgttccaag
     9541 agagatgggt cgtaagcagc gaattctcgg cgaagtatgt cccactggct acgccattcc
     9601 ttcggatcgc cgctactgac atcgatgacg tacaggagca gccgggtccg ctccacatgg
     9661 cgaagaaaag ccaagccgag accgcagttg tcgtgggctc cttcgataag cccggggata
     9721 tccgccaaga caatgcggtc gtcatccacc gacactatgc ccaaatttgg agaaagcgtc
     9781 gtaaaaggat aggatgcgat cttgggttgg gcattggaca tggcggccag caaactggac
     9841 tttcctgcgt tgggcatacc caccaatccc acgtcggcaa tgagcttcag ctcgcaacgt
     9901 agctctacct ccacgccgac gccccccctt tcggcgaagc gaggagcctt gcggacggcg
     9961 ttggcaaaat gggcgtttcc gcgccctccg cgccctcctc gggcgactaa aagacgatcc
    10021 cccggctcca cgaggtccgc cagaggggtt tccgaccgac ggtcataaat gacggtgccg
    10081 cagggtacat ggatgacgca atcatccccc cggcggccgt gctgattttt cccccggccg
    10141 tgatctccgt cttcggcgcg atagtgacgt tgatactcga aatccgccag cgtatgtaac
    10201 gcggggctgg cctcaatgaa aacgctcccc ccgtgtcccc catttcctcc gtctggaccc
    10261 cctcggggaa tgtacttctc ccgacggaag ctcaagcatc cgtcgccccc ctgtccagcc
    10321 ctgaccgata gttgcgcttc gtcgataaat tgcatgaatg gactccgttt ctcgtttttt
    10381 ggaacgcctc tccctaatat ttcgacgccg ggtggcggcg gccgttccgt cgaagtaacc
    10441 ctgaagtccc ttcccatcgg tggtctccgc ttcccgggga gaagtatctc tgggaaggat
    10501 ccctttgcta cagggaaagg cgagggatag agggacggcg gcgaaagggg cttaggaccc
    10561 ccttcttggc cggggagtag atccgtatca cttggtctat cgccaccgcc agccgtgcgg
    10621 aataagggct caggaagatg gcgcgagagg cttatcgatc acgaaaaatc aaacaaaagg
    10681 gggcccgcca aaggccccct ggatggcacc cgtaagctac ggctcacgcc tccgcagcga
    10741 ccacttcaat gcggacgaac ttgtgtcctg ctttggtttc aaaccgaacc agccctgtct
    10801 caagagcgaa aagagtgtca tcccttcccc gtcccacgtg gagtcctggg cggattttag
    10861 ttccccgctg tctcaccagg atgtttcccg caagaacaaa ctgcccatcc tggcgcttca
    10921 ccccaagcat ctttggctga gagtcccgcc cgttcgtgct gcttccctgt cccttcttgt
    10981 gggccaggag ttgcagatca aatactctca tgactttcat gcacctccga aatgcgaaaa
    11041 aaatctccat attgagattc gattatctta aggctttcta gcgccgttcg aataagaact
    11101 ccccagcaag gggtcccccg gatcaggtct tcgggccagg agatcgaaaa aaaaccatgc
    11161 tcaccatccg atgtcaccga gacatccctc agggccaaaa catcctccgc gcccaccact
    11221 aggctttgta gtaacgtaga cacggcggcg cagacgatgt cgcttcccgc ctcgccgaac
    11281 cccgcatgcc cttcggctac caaggctgtg ggattgcccc tgctaaagaa aacggaaaaa
    11341 tggatcactc cccgcaacac tcgctagagg gcgatgcttt cgattcttat ctcagaaaag
    11401 ggctgccgat gtccccggaa gcgacggtag tttttctttc tcctgtagtg aaaaatataa
    11461 accttatccg ccttgccgtg cgagagcacc gtggcccgga cctggactcc ttccacgaag
    11521 ggacttccta cggtcacgcc ctcttcctta gccacaagca agacccggtc aatggaaaaa
    11581 cccgtcccct cggcgaaggg cacccgttcc accttgagaa ctgtcccctc ctccactcga
    11641 tactgcttgc cccctgtttc cacaatagcg tacatgcgtt gctcccttcc gccggatgac
    11701 aggcagtctg gcgacctttt gaagcccgat ccaagcgagt gatggtatag ccaagccatt
    11761 ctaaacatct ctggggctct cgtcaagggt gacggcctgt cctgtgacca gcctgtggta
    11821 ttgtcattcc tgaacgggac gtggataatg tccatcatga tgaatagggg cgacgtgttt
    11881 atgaagacgt atggggcaaa gaaagcgcgg tgattatttg taagtaatta agtttatgat
    11941 taaaatttct cggagggtgg caggcgccgg tcgcaggagg gcaagataaa cgggtgaagg
    12001 caaatttttc ttcgacatgt gtcaacagac agtagcaatg tctatttttt tgacgtcttt
    12061 tgggccaaag aaa
//
LOCUS       DAOOER010000050        12010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_5865_length_12010_cov_9.577666, whole genome shotgun sequence.
ACCESSION   DAOOER010000050 DAOOER010000000
VERSION     DAOOER010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 12010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12010
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5865_length_12010_cov_9.577666"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="PL162_07100"
     CDS             <1..269
                     /locus_tag="PL162_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91820.1"
                     /translation="CPLEQCFRTAWPWPEGDQDLFKSRRRRVVIVDQLTSRGIVGASA
                     LYEAPFSSLHAGGPDVLFAKKPHVLEELFRTLESLASLPPCATG"
     gene            complement(274..1149)
                     /locus_tag="PL162_07105"
     CDS             complement(274..1149)
                     /locus_tag="PL162_07105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HQF91821.1"
                     /translation="MARKALGKGLGALLPGGGEVLREGEGVLRQLPVDALGPNIFQPR
                     QSFDAEALRQLGESLREHGVLQPLLVRRKAPDDGPERYEIVAGERRWRAARAVGLPLV
                     PVRVLELDDTQAAEIALVENIQREDLSPLEVAEALEELVDKHGLTQEETATRIGWSRA
                     AVANKLRLLQLSPPLRAHLQEKRLSEGHLRALLGAPEELREALAERAAQRALSVRDLE
                     NLVRRAEQEGQKSPRPAPALSWAGADRLRERFGWECHVTAQGRHRTLTLKKLTEEQLA
                     EILTLLERHGAEEAP"
     gene            complement(1131..1898)
                     /locus_tag="PL162_07110"
     CDS             complement(1131..1898)
                     /locus_tag="PL162_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HQF91822.1"
                     /translation="MIVIAVANQKGGVGKTTCCVNLGAALGQMGFRILVVDMDPQGNC
                     TSGLGMERGAVERSIYHVLVEGVPLEDVVRPTPWQGVSLAPATIDLAGAEVELVGALS
                     RETRLARPLEKLSGYDVVLIDCPPSLGLLTVNALVGAHQVLVPLQCEYYALEGIGQLL
                     RTLELVRGYLNEKLTLGGIVLTMFDPRTRLAREVVEEARKSFKEQVFSTVIPRNVRLS
                     EAPSFGEPILYYDAASSGAEAYRELGKEVAEKWLEKR"
     gene            complement(1905..2606)
                     /gene="rsmG"
                     /locus_tag="PL162_07115"
     CDS             complement(1905..2606)
                     /gene="rsmG"
                     /locus_tag="PL162_07115"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HQF91823.1"
                     /translation="MTEEKSATVNREEARELLAVGESLLREYVRLLLEENARARLVGP
                     TDPDVIWRSHVLDCAATLPLVRSAPTLLDLGSGGGLPGMVWAACLPKSAVVLCDSVRK
                     KTLALERMARALGLANVRVVPDRSEDLALRERGSFGGVGTRAVAALGVVAEYAAPLVA
                     SGGFALAMKGPAWREEAAEVGPRWRRLGFGAPEIWTYDLGEGLRTVVRWWRTGTPPAF
                     LPRRPGMAEKQPWWR"
     gene            2940..3719
                     /locus_tag="PL162_07120"
     CDS             2940..3719
                     /locus_tag="PL162_07120"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HQF91824.1"
                     /translation="MRLFERILSEGLRQPDSPLQTLMLGWHASAVVLEDGRCGLGAVP
                     LPGQEVLRSTPRFPRHLVEGTAHEVLQLLVSPYPQEFAAASAVASALLPPEEPGESLE
                     TLAPLPEGDQVALLGYDHELVPRLRRWGWHLIIFDDLRPGVDVLPSWTEAPLLRGTPW
                     VWVAFDALRDRRMLALAPLLREHRGAVLQGPGVPWHPPLLRELGFSHLVLPTWTGENF
                     PAILTHISAGGNPWHCAALEWRCFSLHHDPSVLFFPEESSC"
     gene            3713..4522
                     /locus_tag="PL162_07125"
     CDS             3713..4522
                     /locus_tag="PL162_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HQF91825.1"
                     /translation="MLTKKFFRRILLLLLLFVPRCAAGDWRGTVLAVLDGDTVVVRGE
                     DGKEETVRYLYIDTPELHHPRRREEELGPEAREANRDLVEGRGVRLITDAVPRDRYGR
                     LLARVVVDTPRGPVDVGTELVRRGLALPLFIPPNSGGREDIHRALEEARREGRGWWGR
                     ARGRIFSCAQLWAEFSLVAGSFCVLTLRVDEVEERPTRWILRERGSRIPVILPRDFFP
                     SPRSLLGRTLRAWGKAVPSFSGGEMPVADPLQIEVVDNAPPSPEFFPEVWP"
     gene            4795..8430
                     /locus_tag="PL162_07130"
     CDS             4795..8430
                     /locus_tag="PL162_07130"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HQF91826.1"
                     /translation="MAVREEKRPPSRTVGLLVGAAILGLGLGGALFTADFGGKYVLAQ
                     LQGAVWETTGCAVEAEKVSGNPLGGFTLKNLTLSRDGVPFLWAGEMEIFLDLRSLVRG
                     SPAPGLIEVGPLAVDGPRALPLVRSLDAAPSSMPLPSLHLFQLALSLDRHRLLVDRLD
                     LSRRGESFDLRASMTWDLLPLELRGTWDIQGERVLLEESTVKIGAGHGTLQGVLGPEI
                     ALEGSWEGVRLEELAFALPGARSDDCGKIAGRVEVRGTYQDPLISGELSLEEGRLRGI
                     SLDGGFASGVLTPPLLRVKELRGSIQGAPWRGEMSADLSPGRGWVEGRGWGQSVDLAP
                     WGPLGILPPGLQGGLERVDLFFQLRDDGATGRIFFRGLEASLGAWEIAEGRCLGSLSP
                     QGVTFQGSGRWLGSPVKVSGDVMDPLGAPRISASIALVDLPLDRLGEPFPTLRPEGLK
                     GRASLNATLRGSLEAPELQGRLGASALEVRGMGLENVRGDFLIKNGRLELSRGEGTWR
                     KSPLTASGVVEATRCDLRGTAENLPSRALVELIPALADLAPGGTNSVQWKVEGPLSRP
                     RGEVTLRSPQASLRQVPLGSLVMEGSWEGDRFTLRRCQAETAERSLSMKGSLSLGKNP
                     EVDLRGSFKGLYFRDLKPLFPGELSADARLLGEFAFRGPLSRPEAEATFAAERIKGLP
                     VDLTGIKGELSLTSGRLTVRRLTASGADGGTAALSGHVLLGGDPTYFIHGTLQDIPLQ
                     NTHSFLGDRAGLFLRGKLSGTLSVDQRGQSVVLRFRGAVPRLIVNGLSFSDVTGTLGT
                     DGKTIHLAPLRGAVGPGWVTASVDASPEGEKEGWRVDFTARGQDLEVESLLQNAGKSA
                     PSGLQGRFFARIQGRLSGSSLKGEGSLGSSSLALHGFTVNDLTVPLRFDGKGISVEKA
                     RAQVYGGTMEASGRLAFQGGWNASVQISSADVSPLLKDVAPLSGDVSGRGNLKAILSG
                     RLGNLYATEAQLAAEVVEGRISGFSAAQAMASATDGGKSVAFSSIRSTMTVNGRVLYI
                     LPGSRMAAPPGDSSYRYLMVDGSLPLTGKPMNLSCLGEINARSLNTFLGALKGLMDAA
                     QNTEHLVQDVILGGLVGGTARRDFRQVSFKVKGPLEDPVISDLSVSGKIKTGLNPPLV
                     PGEVTGPTNGSGPKGQSGSDISLTLSFPTGKGSAVSDDVEGQLQQQLLEMLLKQVIPG
                     DAPKPTE"
     gene            8519..9316
                     /locus_tag="PL162_07135"
     CDS             8519..9316
                     /locus_tag="PL162_07135"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQF91827.1"
                     /translation="MKYQLIALDLDGTLLRSDNTLSPRTIDAVRRAVKAGAVVAVATG
                     RMYASARDVAERFGVPLPIVAYNGALATVPSRKEPLVSFPLPRLLAGEIMRFFRDNGW
                     YIQSYLGEDFCVERLDSRARSYEKLAGLPPVPLGEALFSPTEDPFKLLAMADTPEEAR
                     EMRRATKERFGEAISAVLSTALFLDVGHPQASKGYALERIGEFYQIPRERTMAMGDSE
                     NDHTLFSAAGYGIAMKNAQEELKLLADFVAESNDDDGVAQVLEGLFP"
     gene            complement(9470..10486)
                     /locus_tag="PL162_07140"
     CDS             complement(9470..10486)
                     /locus_tag="PL162_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008708842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HQF91828.1"
                     /translation="MEKKVLHIISHTDLDGVTAAAVAWHASFPEEAPLRVSLVGYGDV
                     DALVLETLRTGLRPRVIDLFCQRQETVDHLDKFFEEGAEPFFFDHHKTTEERFGNRPW
                     ALVDPSCCAAMVYHRWALERATDPAVKKRLRALTEMVAVANDRDLWLGQDERSRLWQA
                     LVTLCGPWGVLMRLGANPSPDLSSGERQAAESFVERQEIRFSSALEKVTRFGSDLTWV
                     GPDLLEFGDVSDFCGLVLDRMDNPPRLVAVAHRRPGGDWAVSLRSRDSFAARLVGLLK
                     DGKKIRGGGHDDAAAVYFPASYREIQVQDAVLAALRMAEEREKPLEMTLGDLFRPGVS
                     SEKR"
     gene            10798..>12010
                     /gene="dnaA"
                     /locus_tag="PL162_07145"
     CDS             10798..>12010
                     /gene="dnaA"
                     /locus_tag="PL162_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HQF91829.1"
                     /translation="MQESVDILWNSLLRRAREEFPEGTVDVWLSTCLPLSLEDGTFSV
                     DVPNVFVKEQIQSRFLPALESAARKERIAEHVVFHVAAEVRGDEQKRAERAAQPSQKS
                     KAGINNQYIFDTFVVGKSNRLAHASSLAVAESPGGAYNPLFIWGGSGLGKTHLMHAIA
                     HHVMDKTPASRVVYVSAEKFMNELISAIQNNRTQDFKNKYRNVDLLLIDDIQFLANRE
                     STQTQEEFFHTFNSLYDAKKQIVISSDRPPTEIQRIEERLVSRFAWGLVSDIQPPDLE
                     TRMAILGKKAEMRDYKVPEDVLHFLAQNIPSNIRELEGALNRVMACSQLNMESITIDR
                     VALWLKDILRNVARGPVTIDLIQQYVAETFNLSVEDLSGTRRTAEVALARQVAMFLCR
                     SHTEASLQQIGF"
BASE COUNT         2071 a         3876 c         3843 g         2220 t
ORIGIN      
        1 catgtcctct ggaacaatgt tttcgtactg catggccctg gcctgagggg gatcaggatc
       61 tcttcaaaag ccggcgacgg cgggtggtga tcgtggacca actcacgtcc cggggcatcg
      121 tgggggcttc ggcactctac gaggccccct tcagctccct gcacgccgga ggccccgacg
      181 tgcttttcgc aaagaaaccc catgtcctcg aagaactctt ccgcacgctg gagtctctgg
      241 cttctctccc tccgtgcgcc accggatgac cccctagggg gcttcctccg cgccgtggcg
      301 ttccagcagg gtcaggatct ccgcaagctg ctcctcggtg agctttttca gggtgagggt
      361 gcggtgcctt ccctgggcgg tgacgtggca ttcccagccg aagcgctccc ggagccggtc
      421 cgcccccgcc caggaaaggg ccggggcagg acggggggac ttttgccctt cctgttccgc
      481 ccgccgcacg aggttttcca gatcgcgcac ggagagggcg cgctgggccg cccgttccgc
      541 cagggcctcg cgcagttcct cgggggctcc cagcagggcc cggagatgcc cctcggagag
      601 gcgcttttcc tgaaggtggg cccggagcgg aggggagagc tgaagcaggc gcagcttgtt
      661 ggccacggcg gcccggctcc acccgatgcg ggtggcggtt tcttcctggg tcaggccgtg
      721 tttgtccacc agctcctcca gggcctccgc cacttccagg ggggagaggt cttcccgctg
      781 gatgttctcc accagggcga tctccgccgc ctgggtgtcg tcgagctcca gcacccgcac
      841 gggcaccagg ggcaatccca ccgcccgggc cgcccgccag cgccgttccc ccgccacgat
      901 ctcgtagcgc tcaggcccgt cgtcgggggc tttccggcgc accagcaggg gctggagaac
      961 gccgtgctcc cgcagggatt cccccagctg ccgcagggct tcggcgtcga agctctgacg
     1021 gggctgaaag atgttgggcc ccagggcgtc caccggcagc tgccggagca ccccctcccc
     1081 ttcccgaagc acctcgcccc ctccgggcag gagggccccc agccctttgc ctagcgcttt
     1141 tcgagccatt tttccgccac ttcctttccc aactcccgat aggcctccgc gccggagctg
     1201 gcggcgtcgt agtacaggat gggttcgccg aaactggggg cctcggagag gcgcacgttc
     1261 cggggaatca cggtggaaaa gacctgctcc ttaaaggact tccgggcttc ttccaccacc
     1321 tcccgggcca gccgggtccg ggggtcgaac atggtgagga cgatgccccc cagggtgagt
     1381 ttttcgttga gatacccccg gacgagctcc agagtgcgca ggagctggcc gatgccctcc
     1441 agggcgtagt actcgcactg aaggggcaca agcacctggt gggcccccac cagggcgttc
     1501 acggtgagaa gccccaggga gggggggcag tcgatgagga ccacgtcgta cccggagagt
     1561 ttttccaggg ggcgggcgag gcgggtctcc cggctcaggg cccccacgag ttccacctcg
     1621 gcccccgcca ggtcgatggt ggcgggggct aaagacaccc cctgccaggg ggtggggcgc
     1681 accacgtcct ccaggggaac gccctccacg aggacgtggt aaatgctacg ctccacggcc
     1741 ccccgctcca ttcccaggcc gctggtacag tttccctggg gatccatgtc caccaccagg
     1801 atccgaaatc ccatctgccc cagggccgcc cccaggttaa cacaacaggt ggtctttccc
     1861 acgccgccct tttgatttgc caccgctatc acgatcaacg accttcacct ccaccacggt
     1921 tgtttttccg ccatgccggg acggcggggc aaaaacgccg gcggggtacc ggtgcgccac
     1981 cagcgcacca cggtgcggag cccctctccc agatcgtagg tccagatttc cggagcgccg
     2041 aagcccagac gcctccaccg ggggcccact tccgccgcct cttcccgcca ggcggggccc
     2101 ttcatggcca gggcaaagcc cccggaagcc accagggggg cggcgtattc cgccaccacc
     2161 cccagggccg ccacggcccg ggtgcccacg ccgccaaagc tcccccgctc ccggagggcg
     2221 aggtcttccg accggtcggg gaccacccgc acgttggcca ggcccagggc ccgggccatg
     2281 cgctccaggg cgagggtctt cttgcggaca ctgtcgcaga gcaccacggc gcttttgggc
     2341 agacaggcgg cccacaccat gccgggaagc cctccgccgc tccccagatc cagaagggtg
     2401 ggggcggatc gcaccagggg gagggtggcg gcgcagtcga ggacgtggct ccgccagatc
     2461 acgtcggggt ccgtggggcc cacgaggcgg gcccgggcgt tttcttccag gaggaggcgc
     2521 acgtactccc gcaggagact ttcccccacc gccaggagct cccgggcttc ctcccggttc
     2581 accgtggcgc ttttttcttc cgtcatggca tcctccttgc gacctctatg atcctctccg
     2641 gggcggaggg gagttccctc gctcgccccc cgcccccccc gcccttccgg cggaggggcg
     2701 tttttccggg ctctctgccc tccgacaacg gccttctttt ccgaccctgg ccgggccgtt
     2761 ccttcgggga tcggggaccc cacgcaggag agagcccctc cacggaggaa tctcccccct
     2821 gaaacgtcct ccggcgcgac cttttgcgct agtatgggta tcatcttctc caaaggagcc
     2881 tttttttgcc cccttggagg gaagcccggc tccgccgggt ctggggaggt ggaattttcg
     2941 tgcgtctttt tgaacgtatt ctttccgagg ggctgcggca gccggacagc ccgctgcaaa
     3001 cgctcatgtt gggatggcac gccagcgccg tggtgctgga agacgggcgg tgcggcctcg
     3061 gcgccgtgcc ccttccgggt caggaggtgc tccggagcac cccccgcttt ccccggcacc
     3121 tggtggaggg caccgcccac gaggtgctcc agcttctggt ttccccctat ccccaggagt
     3181 tcgccgccgc gtcggcggtg gcttcggccc tcctgcctcc ggaagagccg ggggagtccc
     3241 tggagaccct ggcgcctctt cccgaagggg accaggtggc ccttctgggg tacgaccacg
     3301 agctggtgcc ccggctgcgg cgctggggat ggcatctgat catcttcgat gacctccgtc
     3361 cgggggtcga tgtgctccca tcctggaccg aggcccctct gctccggggg accccctggg
     3421 tgtgggtggc cttcgacgcc ctccgggacc ggcgcatgct cgccctggcc cccctgctcc
     3481 gggaacatcg gggggcggtg ctccaggggc ccggggtgcc ctggcacccc cccctgcttc
     3541 gggagctggg gttcagccat ctggtgctcc ccacctggac cggagaaaac ttccctgcca
     3601 ttctcacaca tatttccgcc ggaggaaatc cctggcactg cgccgccctc gaatggcggt
     3661 gcttttcgct gcaccacgat ccttcggtgc tcttttttcc cgaggagtcg tcgtgctgac
     3721 aaaaaagttt tttcgccgca tcctcctcct gctgctgctt tttgtgccac gctgcgccgc
     3781 cggggactgg cggggcaccg tgctggcggt gctggacggg gacacggtgg tggtgcgggg
     3841 tgaagacggc aaggaggaga cggtgcgcta tctgtacatc gacacgccgg agctccacca
     3901 cccccggcgg cgggaggagg agctgggccc cgaggcccgg gaggcaaacc gagatctggt
     3961 ggagggacgc ggggtgcgcc tgatcaccga cgccgtgccc cgggaccgct acgggcgcct
     4021 gctggcccgg gtggtggtgg ataccccccg gggccccgtg gatgtgggca ccgagctggt
     4081 gcggcggggg ttggcgctcc cccttttcat tcctcccaac tccggggggc gggaagatat
     4141 ccaccgggcc ctggaggaag cccggcggga agggcggggg tggtggggcc gggcccgggg
     4201 gcggattttc tcctgcgccc agctctgggc ggagttttcc ctggtggcgg ggtctttttg
     4261 cgtgctcacg ctgcgggtgg acgaggtgga agaacgcccc acgcggtgga ttcttcggga
     4321 aaggggatct cgcattcccg tcatccttcc ccgggatttt tttccttcgc cccggagcct
     4381 tttggggcgc acccttcggg cgtggggaaa ggccgtcccc tccttctccg gcggcgaaat
     4441 gcccgtcgcc gatccgctcc agatcgaagt tgtggacaac gctccgccct ccccggagtt
     4501 ttttccggag gtctggcctt gaaagccaag gacccccatc ttatccacaa tagttgtcca
     4561 catatacaca agaggagtgg tggataaaag gcccggggcg agaaaaagtt cgtcgaaggg
     4621 tgataaaatg gcgacgacaa ccccgggagt tcctttcgga aggtgtcgac gaaggaaccg
     4681 gaagacgcgg cgggggcggg gttttgtcac ggcgacgccc ctttctgagg aatcgtggat
     4741 gggagctttt ggaaaaagat ctcgagatat ggcgaagatc gggaggatgg gcgtatggcg
     4801 gtccgcgaag aaaaaaggcc tccgtcccgc acggtggggc ttctcgtggg ggcggctatc
     4861 ctgggcttgg gcctgggggg ggcgctcttt acggcggatt tcggcgggaa atacgtcttg
     4921 gcccagctgc agggggcggt gtgggaaacc acggggtgcg cggtggaggc ggaaaaggtc
     4981 tcgggcaacc ccctgggggg ctttaccctg aagaacctca ccctatcccg agatggggtc
     5041 cccttcctgt gggcggggga gatggagatc ttcctcgacc tccgctccct ggtgagggga
     5101 tctcccgccc cggggctcat cgaggtgggc cccctggcgg tggatggccc ccgagccctg
     5161 cccctggtgc gatccctgga cgccgccccc tcttccatgc cccttccttc cctccacctc
     5221 ttccagctcg ccctctccct ggaccgccac cggctcctcg tggatcgcct cgacctgagc
     5281 cgacgggggg aatccttcga cctccgggcc tccatgacat gggatctcct ccccctggag
     5341 ctccggggaa cctgggacat ccaaggggaa agggtcctct tggaagaaag cactgttaaa
     5401 ataggcgcgg ggcatggcac cctccaggga gtcctggggc ccgagatcgc cctggaggga
     5461 tcctgggagg gggtgcgcct ggaggagctg gccttcgccc tccccggggc acgaagcgac
     5521 gactgcggaa agatcgccgg gagggtcgaa gttcggggga cgtaccaaga ccccctgatc
     5581 tcgggggagc tatccctgga agaggggcgc ctccggggga tctccttgga cgggggcttc
     5641 gcctcggggg tcctgacccc tcccctgctc cgggtgaagg agctacgggg gtcgatccag
     5701 ggggccccct ggaggggaga gatgtcggcg gacctctccc ccggaagggg ctgggtggag
     5761 gggcgaggat ggggacagag cgtggacctg gccccctggg ggcccttggg gatcctgccc
     5821 ccggggctcc aggggggctt ggagcgggtg gacctcttct tccagctccg ggacgacggc
     5881 gccacggggc gaatattttt ccggggcctc gaggcatccc tcggagcctg ggagatcgcc
     5941 gaggggcggt gcctgggctc cctttccccc cagggagtga ccttccaagg gtcggggcgg
     6001 tggctgggat cgccggtgaa ggtgagcgga gacgtgatgg accccttggg ggccccccgg
     6061 atcagcgcct ccatcgccct cgtcgactta cccctggatc gcctggggga gccctttccc
     6121 acccttcggc ccgaaggact gaaggggagg gcctccctga acgccacgct ccggggaagc
     6181 ctggaagccc ccgaacttca gggacggctc ggcgccagcg ccctggaggt ccgggggatg
     6241 gggctggaaa acgttagagg agatttttta attaaaaatg gccgtctgga actctcccgg
     6301 ggagaaggga cgtggcgcaa aagccccctg accgcctcgg gggtggtgga agcgacgcgg
     6361 tgcgacctcc ggggcacggc ggaaaacctc ccctcccggg cgctggtcga gctcatcccc
     6421 gccctggccg acctggcccc cggggggacg aattccgtcc agtggaaggt ggaagggccc
     6481 ctttctcgcc cccggggaga ggtgacgctc cgcagtcccc aggcctcgct gcgccaggtt
     6541 cccctgggct ccctggtgat ggaggggtca tgggagggcg accgcttcac cctccggcgc
     6601 tgccaggcgg agacggcgga gagatccctg tcgatgaaag gaagcctttc cctggggaaa
     6661 aaccccgagg tcgatctgcg gggatccttc aaggggttat acttccgcga cctgaagccc
     6721 ctttttcccg gggaactttc ggcggacgcc cgtctcctgg gggagtttgc cttccggggg
     6781 cccctctccc gccccgaggc cgaggcgacc tttgcggcgg agcggatcaa ggggctcccc
     6841 gtggacctca cggggataaa gggcgagctc tccctgactt cggggcgcct cacggtgcgg
     6901 cgtctgacgg cctccggagc cgacgggggc acggcggccc tttcgggaca cgtccttctg
     6961 gggggcgacc cgacgtactt catccacggc accctccagg acatccccct ccagaacacc
     7021 cactccttcc tgggggaccg ggcggggctc ttcctccggg gcaagctctc gggcaccctc
     7081 tccgtggatc aaaggggaca gagcgtcgtc ctgcgcttcc ggggggcggt gccccggctc
     7141 atcgtcaacg gcctttcttt ctccgacgtc accggcacgc tgggcaccga cgggaagacc
     7201 atccacctcg cccccctccg gggcgccgtg ggcccggggt gggtcaccgc cagcgtggac
     7261 gcctcccccg agggggaaaa agaggggtgg cgcgtggatt tcaccgcccg gggacaggat
     7321 ctggaagtgg agtctttgct tcaaaacgcg ggaaaatcgg ccccatcggg gctccaaggg
     7381 cggttttttg ctcggatcca ggggcgcctt tcggggtctt ccctaaaagg agaaggatcc
     7441 ctgggaagtt cctccttggc cctccacggc tttaccgtca acgacctcac ggtgcccctg
     7501 cgcttcgatg gaaaagggat ctccgtggaa aaggcccggg cccaggtcta cgggggaacg
     7561 atggaggcct cggggcgcct ggccttccag ggggggtgga atgcctcggt ccagatctcc
     7621 agcgcggacg tctctcccct gctcaaggac gtggccccct tgagcggtga cgtgtccggt
     7681 cgggggaacc tcaaggccat cctctcgggc cgcctgggaa atctctacgc caccgaggcc
     7741 cagctggcgg cggaggtggt ggagggacgc atctcggggt tttccgccgc ccaggccatg
     7801 gcttccgcca ccgacggggg aaaatccgtg gccttttctt ccatccgaag caccatgacg
     7861 gtgaacggcc gggtgctcta catcctcccc gggagccgca tggccgcccc tccgggagat
     7921 tcctcctacc gatacctcat ggtggatgga tccctgcccc tcacgggaaa gcccatgaac
     7981 ctgagctgcc tgggggagat caacgcccgt agcctcaaca ccttcctggg ggccctaaag
     8041 gggctcatgg atgcggccca gaacacggaa cacctggtcc aagacgtgat tttgggggga
     8101 ctggtgggag gaacggcccg gcgggacttt cgccaggttt ccttcaaggt gaaaggtcct
     8161 ctggaagacc ccgtgatctc cgacctgtcg gtctcaggga agataaagac ggggctcaat
     8221 ccccccctgg tgccgggaga agtgacgggg cccaccaatg gatcggggcc caagggccaa
     8281 agcggaagtg atatctccct caccctctcc ttccccacgg gaaagggttc cgccgtgagc
     8341 gacgacgtgg aggggcaact ccagcaacaa cttctggaaa tgctactaaa acaagttatc
     8401 cccggagatg cccccaagcc caccgaatag cccgaggccc cgaagcgccc gggaagagga
     8461 tccgagatca tcgaaggact cgaaggactc gaaggactcg aaggaaagga aagataccat
     8521 gaaatatcag ctaatcgccc tggacctgga cggcaccctg ctccggtcgg acaacaccct
     8581 gagcccccgc accatcgacg ccgtgcggcg ggccgtgaag gcgggggcgg tggtggccgt
     8641 cgccacgggg cgcatgtacg cctccgcccg ggacgtggcg gaacgtttcg gcgtgccctt
     8701 gcccatcgtg gcgtataacg gcgccctggc caccgtgccc tcccgaaagg aacccctggt
     8761 gtctttcccc cttccccgcc tcctggcggg agaaatcatg aggttctttc gggataacgg
     8821 atggtacatc caaagctatc tgggggagga cttctgcgtg gagcggttgg actcccgggc
     8881 ccggtcctac gagaagctgg cgggcctccc tcccgtgccc ctgggggagg ccctcttttc
     8941 ccccaccgaa gaccctttca agctcctcgc catggcggac acccccgagg aggcccggga
     9001 aatgcgccgc gccaccaagg agcgtttcgg cgaggcgatt tctgcggtgc tttccacggc
     9061 gctttttctc gacgtgggtc acccccaggc ctccaagggg tacgccttgg aaaggatcgg
     9121 ggagttctac cagatccccc gggaaaggac catggccatg ggggattcgg aaaacgacca
     9181 caccctcttt tccgccgccg gatatggcat cgccatgaaa aatgcccagg aggagctgaa
     9241 gctcctggct gattttgtgg cggaatccaa cgacgacgac ggagtggccc aggtgttgga
     9301 aggactcttt ccttaatggc accgccataa aatcctgggc gccaccgtgg cggaaaactc
     9361 caccggtaaa ccgccatccc cgggcaaaag gtgtgaggaa cccggtggac gatggatccg
     9421 cgataccttc gccaaaagtt cccttctttt ttatattttt tatgacagat taccgttttt
     9481 ctgaagaaac ccctgggcga aagaggtctc ccagggtcat ttctaggggt ttttcccgtt
     9541 cctccgccat gcgcagggcg gctaaaaccg cgtcttggac ctggatctcc cggtaggagg
     9601 cggggaaata gaccgccgcc gcgtcgtcgt gcccccctcc tcggatcttt tttccgtctt
     9661 tcagcagtcc caccagtcga gcggcgaagg aatcccggct ccggagggac accgcccagt
     9721 cccccccggg acgacgatgg gccaccgcca ccaggcgggg agggttatcc atccggtcca
     9781 ggaccaggcc gcaaaagtcg gacacgtccc cgaactccag gagatcgggt cccacccacg
     9841 tgaggtcgga gccaaagcgt gtcaccttct ccagggcgga ggaaaaacgg atctcttgtc
     9901 gttccacgaa ggactccgcc gcttggcgtt cccccgagga gagatcgggg gaggggttgg
     9961 cccccaggcg catgagcacc ccccagggac cgcagagggt caccagggct tgccataggc
    10021 ggctcctttc gtcttgtccc agccaaaggt ctcggtcgtt ggccacggcc accatctccg
    10081 tcagggccct aagacgcttt ttcacggcag gatctgtggc ccgttccagg gcccaccggt
    10141 ggtataccat ggcggcgcag cacgaagggt ccaccagggc ccagggacgg ttgccgaaac
    10201 gctcctccgt ggtcttgtga tgatcgaaaa agaacggttc cgccccttct tcgaagaact
    10261 tgtccagatg gtcgacggtc tcttggcgct ggcaaaagag gtcgatgacg cgggggcgga
    10321 gccccgtacg aagggtctcg agcaccagag catccacgtc tccgtatccc acaaggctca
    10381 cccgtaacgg cgcttcttcg ggaaacgatg cgtgccacgc caccgccgcc gccgttacgc
    10441 catcgaggtc ggtgtggcta atgatgtgga gaactttttt ttccatggga gagcaccgtc
    10501 ctttcccccc cagtatagga ttcttttcct tttgtggata ggaaaacttt tctgagggtt
    10561 cttatccaca tctgtggaca aggctgtgga taactttccg ccattggaga atcccttcgg
    10621 aggacgtttt tttgaaagcg ttcttccggg gccttcccct tcttttcctt ggaacgagga
    10681 gaaaggcgtt atccacagaa ctatcctcgg gagaaaaatt ttgtggataa gtttttcccc
    10741 aggccgatgt gctacaatgg gctttccgac ctcccccatc ccgaggtgaa ctaaaacatg
    10801 caggaaagcg tggacatcct atggaactcc ctccttcgcc gggcccggga agaatttccc
    10861 gaaggcacgg tggacgtgtg gctttcaacc tgtcttcccc tgtctttgga ggacggaact
    10921 ttttccgtgg acgtccccaa cgtcttcgta aaggaacaaa ttcaatcaag gtttcttccc
    10981 gccctggaaa gtgccgcccg aaaagagcgc atcgccgaac acgtggtctt tcacgtcgcc
    11041 gcagaagttc gtggagacga acaaaaaagg gccgagcggg cggctcaacc atctcaaaaa
    11101 tctaaagctg ggattaataa tcaatacatc tttgatacct tcgtggtggg aaaatccaac
    11161 cgactggccc acgcctccag tctggcggtg gctgaatctc ccggaggagc ttacaacccc
    11221 cttttcatat ggggtggatc ggggttggga aaaacccacc tcatgcacgc catcgctcac
    11281 cacgtcatgg acaaaacccc cgcctcccgg gtggtatacg tgagcgcaga aaagtttatg
    11341 aatgaactca tctctgctat acaaaataac cgcacacaag attttaaaaa taaataccgt
    11401 aatgttgatc tcctacttat tgacgatata caatttttag ctaaccgaga aagtactcag
    11461 actcaagaag aattttttca tacttttaat agcctttatg acgcaaaaaa acaaattgtt
    11521 attagctcgg atcgacctcc cacagaaatt caacgcatag aagaacgtct cgtaagccgt
    11581 tttgcctggg ggttggtaag cgatattcag ccacctgacc tagaaacacg catggctatc
    11641 ttaggtaaaa aagctgaaat gagagactat aaagttcctg aggatgttct tcattttctt
    11701 gcccaaaata ttcctagtaa tatccgggag ctagagggag cattaaatag agtaatggct
    11761 tgttctcaac ttaatatgga gagtattact attgaccgag tggccttgtg gcttaaagac
    11821 atcctgcgca acgtggcccg agggccggtg actattgatt taatccaaca atacgtggcg
    11881 gagaccttta atcttagcgt ggaagacctt tcgggcactc gacggaccgc cgaagtggcc
    11941 ctggctcgtc aggtggccat gttcctctgc cgctcccaca ccgaggccag cttgcaacag
    12001 attggttttg
//
LOCUS       DAOOER010000051        11998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_5876_length_11998_cov_9.866030, whole genome shotgun sequence.
ACCESSION   DAOOER010000051 DAOOER010000000
VERSION     DAOOER010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 11998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11998
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5876_length_11998_cov_9.866030"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            131..700
                     /locus_tag="PL162_07150"
     CDS             131..700
                     /locus_tag="PL162_07150"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HQF91830.1"
                     /translation="MRQALVRFLVDLAQRPSSFAKKLGWITLGMGTFLFLSPWLLGKG
                     VRALGQGLAPFLGVIEGAVGILGLGAGLSIVGWVIAVQWRLGAGTPMPAAPTRKVVTS
                     GPYALCRHPMYFAAVLYHLGFVTMTSGLGPGVAAAGGVAAFVVFYARTVEERELEARF
                     GEEYRAYRARTPFLFPRGKDLWEVLRRVF"
     gene            complement(804..1790)
                     /locus_tag="PL162_07155"
     CDS             complement(804..1790)
                     /locus_tag="PL162_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HQF91831.1"
                     /translation="MGTEKTHTMDFGEIDGILVGHAQNLEGPTGVTVVLCPEGASAGV
                     DVRGGAPGTRETDLLDPSNLVERVHAVFLAGGSAFGLDAAAGIMAFLEGHGVGFDVGV
                     TRVPVVCGAVLFDLLLGDHRIRPDRDMGYRACEAAARGPVPQGSVGAGAGATIGKIRG
                     MERAVKSGIGSGACRGGDLQVGALVAVNALGDVVDPASGRRVGGLRREDGVGWEDTEE
                     LMIASGAPTGNLFGGNTTIGVVATNARFSKAQARKLASMAHDGYARTLRPASAMVDGD
                     TIFALATGRVEADLTTVGILAARAVAQAVVRAVRSATSMAGVPAVRDLPWAE"
     gene            complement(1800..2159)
                     /locus_tag="PL162_07160"
     CDS             complement(1800..2159)
                     /locus_tag="PL162_07160"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQF91832.1"
                     /translation="MIWKLSALTPTWREGLQKGNGGAWCFYPPAGELKSPGSRVRMMG
                     RLALDPGASIGPHMHTENEEVYIILSGSGRYLDGGEYHDVGPGDVTLTSPEGPHALEN
                     TGTEPLVFVALFAEKES"
     gene            2738..2825
                     /locus_tag="PL162_07165"
     tRNA            2738..2825
                     /locus_tag="PL162_07165"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2771..2773,aa:Leu,seq:caa)
     gene            complement(2930..3532)
                     /locus_tag="PL162_07170"
     CDS             complement(2930..3532)
                     /locus_tag="PL162_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91833.1"
                     /translation="MRKIRVIGACLLVLGLATGAWGAPSKKELQAMGLFLSNFTELGF
                     TEIVTEDMARPENYPDLIRFGIMHNYVNNFKSRIEKNKEGAQGYGDFRIKASWVEESL
                     RRYFGLEVAANKSVDASDPPYHFDGKHFHFWASDGEAPWYARVERATSPQKGLWELSG
                     VVYNADDPEELLGDFSAVIREGLWKGKLHYVLVRLKTEVR"
     gene            complement(3610..4350)
                     /locus_tag="PL162_07175"
     CDS             complement(3610..4350)
                     /locus_tag="PL162_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91834.1"
                     /translation="MRKALWTLLGALLGMGALVGVALAAQETYELRDGEIYRIDAAGK
                     ATLVKDMEPGRFGTDKGVYAWILVDGELSEAMVGSEKGIYFFDEGEKPLGFLPFDGAG
                     FCDISFSSDAAQMIVNFGADVSQEVTLYDFVGFKKKATFRAIGSATWIDHARFAFTKE
                     DRSKGPRGASAPHQEGWVSVVVYDTAIEEEVPVMEATATKDYLLDGIDYEESTMTIIE
                     RSVKDAKDWNDEQKVRDNVITAPIPAAG"
     gene            complement(4482..4916)
                     /locus_tag="PL162_07180"
     CDS             complement(4482..4916)
                     /locus_tag="PL162_07180"
                     /inference="COORDINATES: protein motif:HMM:NF018037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1036 domain-containing protein"
                     /protein_id="HQF91835.1"
                     /translation="MTWRRGRGWTLALLMAVGVFGMARGAGAAAIILDNYRDQKISVA
                     LYYYDTSEGSWCCQGWWGVDAGGERTITVPHDPGERIYYYVQAGGKVVHKRSEGWSRQ
                     HVVDDAFKYYEHDGCPDGTNYRAVYFNGSKKATGKSWRLKIN"
     gene            complement(4913..5170)
                     /locus_tag="PL162_07185"
     CDS             complement(4913..5170)
                     /locus_tag="PL162_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HQF91836.1"
                     /translation="MAYIRSVMPLSDWRLLVEMETDSVIVVDLRRKLATARYGDLKDP
                     RLFRSVTTDGEGVSWGGGRVAVTARELMDVICVDMRKENDG"
     gene            complement(5207..6106)
                     /locus_tag="PL162_07190"
     CDS             complement(5207..6106)
                     /locus_tag="PL162_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91837.1"
                     /translation="MGRKTWAWMVVAALGCTAAGAEVRALTVPVGPGAPTVTVTHEKG
                     TVEVKEGDSLIQKISYDQEIEGDLQESGVDLVQGEDMNFDGFLDLRITESVGVPNAHY
                     ACLLWNHKTKKFEKNEELGGLSNPEFDPETKKIKTFEHARATDNIYEEYEWREGTLTV
                     LWRKIQGYDDERERFFVREERRKEGGEMVQESEHYMTEAALEIYLETGEIPLLDEALR
                     GKIALAAKALLGADLAGEGELHGVGSREGQPVRAWVFPLTKGEEACFETSSGGTIYLN
                     KGDGGTFAVHFDGNAVTLGEKAD"
     gene            complement(6212..8917)
                     /locus_tag="PL162_07195"
     CDS             complement(6212..8917)
                     /locus_tag="PL162_07195"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HQF91838.1"
                     /translation="MPMGHERWLYELREKGLRDIATLQKHARQEGHEEGIDKGIDKGR
                     GEALCASASARFFVPPEAKEKRWDHIATPKYHPKVHYCSERLRSKLLQVPRSRTTFVE
                     APSGAGKTTTFRDHFSSSEEKGVAVRWFVASEEPLTSGWLRLSREIEAIDPQGGERLR
                     RLGLPGEENQGEVAQALMELRCDRETYLVCDNFQHLQRSLPSAVWRALVDHGGRELRL
                     IILTQQLPFRGLAILGNSDVLRIENDDLCLKAEEIGEYYRMAGVELTEAQMRQLHAYS
                     EGWIAALYLQLESFVRTGSLEGKASIYEMMNDLFWRRISREEQEAIFRLSPFDSVTVR
                     QACFLLGVESLPEPLAELLHQGMLIRYDIAERRYFPHAILWDFVRSALLEEPEALRRE
                     VWRRSGEWSAQQGEKTQAIIFFHRLGDFAAILALDLRCVDMSRAILDLGAKEMGSILR
                     DIADRATPELRRDHAFTMISVAFEAFTLGDGPLYERLCREMKDLLEESDLDPARRRSL
                     LGELALTESFGQYNDIEGMGRGHQRAHELLGTHSTLFNPDSPWTLGWPSVLGMYHRTC
                     GGMDEEMAQMDYWIPRYNALTLGNGSGADFVFRAEVELHRGDDAGAERGALKALDVAR
                     HQGQDSLAICAAFLIQRVALLRGDREAWDAGKNLMEQISRSSTYTLSRRIADMAAGFM
                     AVLLDDPEGVPPWIRNGHFMETLSPALPFACTIYGRLLLLTGQERELLLRSEEFLSAA
                     RRQRSLLAEVYGMIAVAAMQGRLGKEDEGEKTLGLALDLALPDGLILPFAENGDLLGG
                     ILGRTLSRSWKEKRPVILALKDRFTRGKEALLQRRRSEGIPGDLTFREHEVARRAAEG
                     QSNREIAEALFLSENTVKHYLKAIFRKMEVDSRRALKEALRSFHP"
     gene            9190..9687
                     /locus_tag="PL162_07200"
     CDS             9190..9687
                     /locus_tag="PL162_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HQF91839.1"
                     /translation="MPFFVVYQDITAMAVDAIVNAANTELRMGGGVCGAIFHAAGAEE
                     LQAACAPLAPIKTGEAVITPGFRLPARYVIHTAGPVYHDGKSGEEELLRSCYFRALTC
                     AVENGCHSVAFPLISSGIYGYPPEEARRVATAAIEDFLTRRDLQVYLVLFGKAADRKT
                     ISPAL"
     gene            complement(9926..>11998)
                     /locus_tag="PL162_07205"
     CDS             complement(9926..>11998)
                     /locus_tag="PL162_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HQF91840.1"
                     /translation="DDGQVEALWGLSRDARGHFVERVELFKNTLSSIKVSLADRLRLE
                     KEVAKLDQAPEGAQVLPPPPYSLSFADAQKDRLEEIAKKKESWRTQIALGEQTLEALL
                     DSRRAAEKEVRLLQDGAPGASPWPLNDAVLRRDIFRALRVLERLRLDNLKIEFRALES
                     QEKRLTQTQQRIVAHLSFDEKDRDAQIGKYQGFMDFLRKEAENLLKQRQQNERALASA
                     REQMARASGDRESRGAALRVAQREAELELTRQRLEHGALMKQVVGVMQGVWRNRYALL
                     RGEIPAEALSGHLEVAVDLTQEIGKQVEIQQQEAWRLQKKLSSLQKELDNPDVPKDFR
                     GIMEQTYRTVADLAEGKREFVSFLLENLGVAENAEAELRAALGKVSLAKKINVAWQEQ
                     FWSVWQMELWVADERPITVGKTVSAVLLTLGGIFANAYLSRRVAHRLERHFNVLPTSA
                     MAVQRLTFYALMVATVLWALATVGIPLTAFAFLGGALAIGFGLGAQTFFSQIISGFLI
                     TIQKPIRIGDRIQIGDAQGKVVLIGSRYTRIRTFDNLDVLVPNEHFLSHPVTNWTLSS
                     TLVRGSLSVKVSCTAPPETVEEILMGSSARHDKILKKPAPFVRFSDFSEGHLAFDLFF
                     WVDLATVDPTEVAHDLRFALMRDFALRGIVLAQPRREVRLDWAPPAEGRRLPGDAGDE
                     GGAPSHRN"
BASE COUNT         1962 a         3887 c         3743 g         2406 t
ORIGIN      
        1 cggccaccgt gcggagaatc ggcggtgcgg tgaccatacg ggaaaccggc ggtgcggcaa
       61 ttgtccgaca acgcccccct ggggtcctgg ggcttgggaa cggggcattc taaaaaaggg
      121 gggggttttc atgcgtcaag ccttggtgcg ttttctcgtc gacctggccc aacggccgtc
      181 ctctttcgcc aaaaagctgg gatggatcac cctggggatg gggactttct tgtttctctc
      241 cccctggctc ttggggaaag gggtccgggc cttggggcag gggctggccc ccttcctggg
      301 ggtcatcgag ggggcggtgg ggatcctggg cctgggggcg gggctttcga tcgtcggatg
      361 ggtcatcgcc gtccagtggc gcctgggggc ggggaccccc atgcccgccg cccccacccg
      421 gaaggtcgtc acctccggtc cctacgccct atgtcgtcac cccatgtact tcgcggcggt
      481 gctctatcac ctggggttcg tcacgatgac ctcgggcctc ggcccggggg tggcggcggc
      541 gggaggggtg gcggccttcg tcgtctttta cgcccgcacc gtagaagaaa gggagctgga
      601 ggcccggttc ggcgaggagt accgggcgta ccgggcccgc acgccctttc tgttcccccg
      661 gggcaaggat ctctgggagg tccttcggcg ggttttttag gcgggctacg gccgtcggcc
      721 gtccggggtc ttcgggacgc cgggggggcg cttcgggccg gggccgaccg gggaggcggg
      781 catcggcgcg atgacggaag agatcactcc gcccagggca gatcccgcac tgcgggcact
      841 ccggccatcg atgtggcgga tcgcaccgcc cgcaccaccg cctgggccac cgcccgggcc
      901 gccaggatcc ccacggtggt gagatcggcc tccacgcgcc ccgtggccag ggcgaagatc
      961 gtgtccccgt ccaccatggc ggaggcgggg cgcagggtgc gggcgtagcc gtcgtgagcc
     1021 atggaggcga gcttccgggc ctgggccttg gaaaatcggg cgttggtggc caccacgccg
     1081 atggtggtgt tccctccgaa gaggtttccc gtgggcgccc cggaggcgat catgagctcc
     1141 tccgtgtctt cccacccgac cccatcttcc cgtcgcaggc cccccacccg gcgccccgaa
     1201 gcggggtcca ccacgtcgcc gagggcgttc accgccacga gggcccccac ctggagatct
     1261 cccccccggc aggctccgct gccgatgccg ctttttacgg cccgctccat gccccggatc
     1321 ttgccgatgg tggcccccgc ccccgcgccc acgcttccct ggggcaccgg gccccgggcc
     1381 gccgcctcgc aggcccggta ccccatgtcc cggtccgggc gtatccggtg atctcccagc
     1441 aacaggtcga agagcacggc gccgcagacg acgggtaccc gggtgacgcc cacgtcgaag
     1501 cccacgccgt gcccttcgag aaaggccatg atccccgccg ccgcatccag gccgaaggcg
     1561 cttcccccgg cgagaaagac ggcgtgcaca cgctccacca ggttcgaggg gtccagcaga
     1621 tcggtttccc gggtgccggg ggcgcctccg cgcacgtcga cccccgccga ggccccctcg
     1681 gggcagagca ccaccgtcac ccccgtgggg ccctccaggt tctgggcgtg ccccactaaa
     1741 ataccgtcga tctcgccgaa gtccatggtg tgggtctttt ccgtccccaa gggaggcgat
     1801 cagctctctt tttcggcgaa gagggccacg aagaccaggg gctcggtgcc cgtgttctcc
     1861 agggcgtggg gtccctccgg gctggtgagg gtcacgtccc cggggcccac gtcgtggtac
     1921 tcgccgccgt ccagatatcg tccggagccg gagaggatga tgtagacctc ctcgttctcc
     1981 gtgtgcatgt gggggccgat ggaagccccg ggatccaagg ccaggcgtcc catcatgcgg
     2041 acccggcttc cggggctctt gagttcccct gcggggggat agaaacacca cgcgccacca
     2101 ttgccctttt gcagtccttc ccgccaggtg ggggtcagtg cgctaagctt ccagatcatc
     2161 gtaccatctc ctcctctgtg gctcgcgcca ggatgggccc tttcggcaag gctccggtca
     2221 ccgggcctcg ccaagggggc tgtgttggag ccattctaac tctcccgagg tgttttgtct
     2281 cgtccttaga cagagggctt ccggcggggg gactttggcg cagggatcgg atgaggtgga
     2341 ggggagtcgg gctcttatgg aactcgaccg catcgacccc tgcctccgca gccgcgtcaa
     2401 cgagccgcag agccatgtcc agcgatccgt cgtggttcac gcccgcctcg gcgattacgt
     2461 agacgctcct taggcttcac ctctccgtta ccagcccttc gctccgtttt aaagagggca
     2521 ggacgaaaga agaggcgaca cttttccgcc ggaggcaaaa agagcgacgc acctcaccag
     2581 ggccaccctc cgaaagaagg gtcgcctaca cgatagagat cgccaagttc tcatcctttt
     2641 tttcagactc tcttcaattt ccccgggaga gatggggcaa taggtggtcc gccgggcctg
     2701 gacggatcgg ccgttttgtg ctaaaaagta cgccagggcc ggagtggcgg aatggtagac
     2761 gcagcggact caaaatccgc cggggttcac gctctgtggg ggttcgagtc ccccctccgg
     2821 caccatattt ttatggaaaa gcgaagagcc cggcggagcc gggctctttt cgtcgtggcc
     2881 cccgggaggg gccaagatcc caaggggagg gcacctccgg gagggggact caacgcacct
     2941 cggtcttcag gcgcaccagg acgtagtgca acttcccctt ccagagtcct tcccttatga
     3001 cggcggagaa gtcccccagg agctcctcgg ggtcgtcggc gttgtagacc actccggaga
     3061 gttcccagag gcccttttga gggcttgtgg ccctttcgac ccgggcgtac cagggagctt
     3121 cgccgtcgga ggcccagaag tggaagtgct tgccgtcgaa gtgatacggg ggatcggagg
     3181 cgtccacgga cttgttcgcc gcgacctcca gcccgaagta gcgccggaga gactcttcga
     3241 cccagcttgc cttgatgcgg aagtcgccgt atccctgggc tccctctttg ttcttctcga
     3301 tacggctctt gaagttgttg acgtagttgt gcatgatgcc gaagcggatg agatcagggt
     3361 agttctccgg gcgggccata tcttccgtga cgatttccgt gaagcccagc tcggtgaagt
     3421 tgctcaggaa aagacccatg gcctggagct ctttcttgct gggggccccc caggcccccg
     3481 tcgccagtcc caggaccagg agacaggccc ctatcactct gatttttctc acgactattc
     3541 ctccttcctg cggccttcct gcggcctcag ggttccttgc catcgccaaa aatcacccgg
     3601 cgcctctttc tatcccgccg ccgggatggg ggcggtgatg acgttatctc ggactttttg
     3661 ctcgtcgttc cagtcttttg cgtctttcac cgatcgttcg atgatggtca tggtgctttc
     3721 ttcgtagtca atgccatcca gcaggtagtc cttcgtggca gtggcttcca tgacgggaac
     3781 ttcttcctct atcgccgtgt cgtagaccac cacggagacc catccttcct ggtgcggggc
     3841 cgaggcccct cggggcccct tgcttcggtc ttctttcgtg aaggcaaagc gggcgtgatc
     3901 gatccacgtc gccgatccta tcgcccggaa ggtggccttt ttcttgaagc cgacgaagtc
     3961 gtacagggtg acttcctggg agacgtccgc gccgaagttc acgatcatct gcgctgcgtc
     4021 ggagctgaag gagatgtcgc aaaatcccgc cccgtcgaag gggaggaagc ccaggggttt
     4081 ctccccttcg tcaaagaagt agatgccttt ctccgatccc accatggcct cggagagctc
     4141 tccatccacg aggatccagg cgtagacgcc cttatccgtg ccgaagcgtc ccggctccat
     4201 gtcttttacc aacgtggcct ttcccgccgc gtcaatccgg tagatctcgc cgtctctgag
     4261 ctcgtaggtc tcttgggccg ccagggccac tcccaccagg gcccccatcc ccaggagggc
     4321 ccccaaaagg gtccaaagtg cctttctcat cgccatcgct tctcctttct tgttttgtcc
     4381 cttcttccgg gacagggttg cccgatctta tccccgtctt tttgtcaccg gatccaagcc
     4441 gcccccacgc cccgctcttc cgggcgtccg gcgaatccgc ctcagttgat tttcaatctc
     4501 caggattttc ccgtggcctt cttgctgccg ttgaagtaaa ccgcccggta gttcgttccg
     4561 tcggggcacc cgtcgtgctc gtagtacttg aaggcgtcgt ccacgacgtg ctggcgcgac
     4621 cacccttcgc tccttttatg gaccaccttg cccccggcct gcacgtagta gtatatacgc
     4681 tccccggggt cgtgggggac ggtgatggtg cgttctcccc ccgcgtcgac gccccaccac
     4741 ccctggcagc accacgagcc ttcgctggtg tcgtaatagt acagagccac cgaaattttc
     4801 tgatctctgt agttgtccag gatgatggca gcggccccgg ctccccgggc catcccaaag
     4861 actcccacag ccatcaacag ggccagggtc catcctcttc ctcttcgcca ggtcatccgt
     4921 cgttctcctt tctcatgtcc acacaaatga catccatgag ttcgcgggcc gtgacggcca
     4981 cgcgccctcc tccccacgac actccttcgc catcggtggt gaccgaccgg aagagcctcg
     5041 ggtctttcag atcgccatac cgggccgtgg ccagcttgcg gcgcaggtcc accacgatga
     5101 cgctgtccgt ctccatttcc acgagcaaac gccagtcgct caggggcatc acagaacgga
     5161 tgtacgccat cttatccctc ctcaactccc caagatgccc cctgggtcag tccgcttttt
     5221 cccccagagt gaccgcgttc ccgtcaaaat ggacggcgaa ggtcccgcca tctcccttgt
     5281 tcaggtagat ggttcctccg gaggaggtct cgaaacaggc ctcttcgcct ttggtcaagg
     5341 ggaagaccca ggccctcacg ggctggcctt cccgggagcc cacgccatgg agctctcctt
     5401 cccccgcgag atccgccccg aggagggcct tcgccgccag ggcaatctta cccctaaggg
     5461 cttcgtccag gagggggatt tctcccgtct cgaggtagat ttccagggcg gcttctgtca
     5521 tgtagtgctc ggattcctgg accatctcgc cgccctcctt ccggcgctct tctcggacga
     5581 aaaaacgctc tctttcgtcg tcgtagccct ggattttgcg ccagagcacc gtcagggttc
     5641 cttcacgcca ctcgtactct tcgtagatgt tatccgtggc ccttgcatgt tcgaaggtct
     5701 ttatcttttt tgtctccgga tcgaactctg gattggagag cccacccaat tcctcatttt
     5761 tttcaaactt cttcgtttta tgattccaaa gtaggcaggc gtaatgggcg ttcggcacgc
     5821 ctacgctttc ggtgatccgc agatccaaga atccgtcgaa gttcatatct tctccttgga
     5881 ccaggtcaac tcccgattct tgcaggtctc cttcgatctc ctgatcgtag gagatcttct
     5941 gaataaggct atccccttct tttacttcca cggttccttt ttcgtgggtc accgtcaccg
     6001 tgggggcccc cggccccacg ggcaccgtca aagcccttac ttccgccccc gctgcggtac
     6061 accccagcgc cgccacgacc atccatgccc aagtctttcg tcccatggtt acattcctcc
     6121 gctccttcga aagatttcgg cgtcccgagc cctcccctag ggggatggta cggggcgccg
     6181 ggaaacgggg ctacaccctt ttcggggggt cttaagggtg aaacgatcgc agggcctctt
     6241 tcagggcccg ccgcgaatcc acttccattt tcctaaaaat cgccttcagg tagtgtttga
     6301 cggtgttttc ggacaggaag agcgcctcgg cgatctcgcg attgctctgg ccttcggcgg
     6361 ctcggcgggc aacttcgtgt tcgcgaaagg tgagatctcc ggggatccct tcgctccggc
     6421 gtctctggag gagagcttcc tttccccggg tgaaacggtc tttcagggcc aggatgacgg
     6481 ggcgtttttc tttccacgac cgggagaggg tccgccccag gattcccccc aggaggtctc
     6541 cgttttccgc aaaggggagg attaggccgt cgggaagggc aagatccagg gcgagaccca
     6601 gggtcttttc tccttcgtcc tcttttccca ggcgcccctg catcgccgcc acggctatca
     6661 tcccgtacac ttcggccaaa aggctccgct ggcgtcgagc ggcggacaaa aactcttcgg
     6721 agcgcagcag gagctcgcgt tcctgccccg tgaggagcag caggcgtccg tagatcgtgc
     6781 aggcaaaggg cagcgccggg gagagggttt ccataaaatg gccgtttcgg atccacggcg
     6841 gaactccttc gggatcgtcc aagagcacgg ccatgaagcc cgccgccatg tcggcgatgc
     6901 gccgggagag ggtataggtc gaggatcgag agatctgctc catgaggttt ttccccgcgt
     6961 cccaggcctc ccggtctccc cggagcaggg ccactctctg gatcaggaag gcggcgcaga
     7021 tggccaggct gtcctggccc tggtggcggg cgacgtccag ggccttaagg gctcccctct
     7081 ctgctccggc gtcgtcgcca cggtgaagct cgacctccgc acgaaagacg aagtcggcac
     7141 cgctgccgtt tccgagggtc agcgcgttgt accgggggat ccagtagtcc atctgggcca
     7201 tctcttcgtc catgcccccg caggtgcggt ggtacatccc caggacggag ggccatccca
     7261 aggtccaggg agagtcgggg ttgaacaggg tgctgtgggt tccgaggagt tcgtgggccc
     7321 gctggtgtcc ccggcccatg ccttcgatgt cgttgtactg tccgaaggat tccgtcaagg
     7381 caagctctcc caggagggag cggcgccggg caggatccag gtcggattct tccaggaggt
     7441 ctttcatttc gcgacagagc ctttcgtaca aggggccatc tcccagggta aaggcctcaa
     7501 aggccacaga gatcatggtg aaggcgtggt ctcgccggag ttcgggcgtg gcacggtcgg
     7561 cgatgtcgcg caggatcgat cccatctcct tggctcccag gtcgaggatg gctcggctca
     7621 tgtcgacaca cctgaggtcc agggccagga tggccgcgaa atcccccagg cggtgaaaaa
     7681 agatgatcgc ctgggtcttc tctccctgct gggcgctcca ctctccggac cggcgccaca
     7741 cttcccggcg cagggcttcc ggctcttcca ggagggccga ccgcacgaaa tcccagagaa
     7801 tcgcatgggg aaagtaccgt cgctccgcga tgtcgtagcg gatgagcatt ccctggtgaa
     7861 ggagctcggc caggggctcc ggcagggact ccactcccag gagaaagcag gcctggcgga
     7921 cggtgacgct gtcgaagggg gaaaggcgaa agatcgcctc ttgttcttcg cgggaaatcc
     7981 tacgccagaa gaggtcgttc atcatttcgt agatgctcgc cttgccttcc agggatcccg
     8041 tgcggacaaa gctctccagc tggaggtata aggcggcgat ccaaccttcg gagtaggcgt
     8101 gaagttggcg catctgggcc tcggtgagct ccactccggc catgcggtag tactcgccga
     8161 tctcctcggc cttcaggcag aggtcgtcgt tttcaatgcg caagacgtcc gaattaccca
     8221 ggatcgccaa gccccggaag gggagctgct gggtgaggat gatcagccgg agttcccggc
     8281 cgccgtggtc caccagggcc ctccatacgg cggagggaag gctcctttgg aggtgctgga
     8341 agttgtcgca gacgaggtag gtttctcgat cgcaccggag ctccatcagg gcctgggcca
     8401 cttctccctg gttctcttcc cccgggaggc caaggcgtcg gagcctttcc cctccctggg
     8461 ggtcaatggc ttcgatctcg cggctcaggc gcagccaccc cgaggtgagg ggttcttcgc
     8521 tggccacgaa ccaccgcacc gccacgcctt tttcttccga agaggaaaag tggtcgcgaa
     8581 aggtcgtggt cttgcccgct cccgaggggg cttcgacgaa ggtcgtccgg gaccggggaa
     8641 cttggagaag cttgctcctc aggcgctccg aacagtaatg gaccttggga tgatatttcg
     8701 gcgtggcgat gtggtcccac ctcttttctt tcgcctctgg gggcacaaaa aatcgggccg
     8761 acgccgaggc gcagagcgcc tctccacgac ctttatcgat gcctttatcg atgccctctt
     8821 cgtgcccttc ctggcgggcg tgtttctgca aggtggcgat gtcgcgcaat cccttttctc
     8881 ggagctcgta aagccaccgt tcgtgcccca tcggcatgaa gagttctttc acaaacgaag
     8941 gggccgtggg ggtaaaaggg gcgtgacagg ggaaacgttt cgcccaaggc tttgacctgg
     9001 tcgtctccct gggcgaaggg gagtcggagc agcggtaaga tgggcgctcc cggcggcggt
     9061 gttttggggg ctcccgatcc tctctccgtt gtgctatctt gaaaggggta ggggcgataa
     9121 gccctttagc ctggcgcacc acgccgggac ggacctaccc ccgccagggg gagtacggag
     9181 gaggttgcca tgcctttttt cgtcgtatac caagacatca ccgccatggc cgtggacgcc
     9241 atcgtcaacg cagccaatac cgagctgagg atgggaggag gcgtgtgcgg ggccatcttc
     9301 cacgccgctg gagcggaaga gcttcaggcc gcctgcgctc ccctggctcc gataaagacc
     9361 ggggaggcgg tgatcacccc ggggtttcgc cttcccgccc ggtacgtcat ccacactgcg
     9421 ggccccgtgt accacgacgg aaaaagcggc gaggaggagc tgctccggtc gtgctatttc
     9481 cgggccctga cctgcgctgt ggagaatggt tgccatagcg tggccttccc attgatttcc
     9541 agcggcatct acggctatcc tccggaagag gcccggcgcg tggccaccgc cgccatcgag
     9601 gatttcttga ctcgacgcga cctccaggtg tatctggtgc tcttcggcaa ggcggcggac
     9661 cgaaagacca tctccccggc cctttaaggc ggcgaggtcc tccgccacca ccgccccccc
     9721 gcatggacga tcccaaacag tagcgccccc attccctttc ttacgaaaca aaggactctc
     9781 cgccatcaag gcggcccttt gttatggcat tatactccgt gcgctgcaga gcgcaaggat
     9841 cgcccttcgt cccggaaggg ccccttgatc ccgtcgcccc acgccccggg gccgggggca
     9901 aggccctgcc cgacgatgat gaggcttagt tccgatggga aggagcgccc ccttcgtctc
     9961 ccgcgtcccc cgggaggcga cgcccctccg ccggaggggc ccagtccagg cgcacttccc
    10021 gccggggttg ggccaggacg atgccccgaa gggcaaagtc cctcatcagg gcaaagcgga
    10081 ggtcgtgggc cacctccgtg gggtccacag tggccaaatc cacccaaaaa aagaggtcaa
    10141 aggccagatg tccttcggag aagtcggaaa aacgcacaaa gggggcgggt tttttcagga
    10201 ttttatcgtg ccgggcactg gagcccatga gaatttcctc cacagtctcc ggaggggcgg
    10261 tgcaggacac tttcaccgag aggctccccc gcaccaaggt actcgacagg gtccagttgg
    10321 tcacggggtg ggacaaaaag tgctcgttgg gcaccagcac gtcgaggttg tcgaaggtgc
    10381 ggatgcgggt gtagcgggaa ccgatgagga ccaccttgcc ctgggcatcg ccgatctgga
    10441 tgcggtcgcc gatgcggatg ggtttctgaa tggtgatgag aaaaccgctg atgatctggc
    10501 taaaaaaggt ctgggccccg agaccgaagc cgatggccag ggcccccccc aggaaggcaa
    10561 aggcggtgag gggaatgccc accgtggcca gggcccagag gacggtggcc accatgaggg
    10621 cgtaaaacgt gaggcgttgc accgccatgg ccgaggtggg gaggacgttg aagtggcgct
    10681 cgagcctgtg ggccacccga cgggagaggt aggcgttggc aaatatgccg ccgagggtga
    10741 gcagcacggc gctgacggtt tttcccacgg tgatggggcg ttcgtcggcc acccagagct
    10801 ccatctgcca gacgctccaa aattgctcct gccaagccac gttgatcttt ttggccaaag
    10861 agaccttgcc tagggcggcc cggagctccg cctcggcgtt ttccgccacg cccaggttct
    10921 ccaggagaaa ggagacgaac tctctctttc cttcggccag gtcggccacg gtcctgtagg
    10981 tctgctccat gatgccccga aagtccttgg ggacgtcggg gttatccagc tctttttgca
    11041 gggaggagag tttcttctgg aggcgccagg cctcttgctg ctgtatttcc acctgcttgc
    11101 cgatctcctg ggtgaggtct acggccactt ccaggtggcc cgatagggcc tccgccggga
    11161 tctctccccg gaggagggcg tatcggttgc gccagacccc ctgcatgacc cccaccacct
    11221 gcttcatgag ggccccgtgt tccaggcgct ggcgcgtgag ctccagctcg gcctcccgct
    11281 gggccacccg gagggccgcc cctcggctct cccggtctcc cgaggcccgg gccatctgct
    11341 cccgggcgga agccagggct cgctcgttct gttggcgctg cttgaggagg ttttccgcct
    11401 ccttccggag gaagtccatg aagccctgat acttgccgat ctgggcgtcc cggtcctttt
    11461 cgtcgaacga gaggtgggcc acgatccgct gctgggtctg ggtcagcctt ttttcttggc
    11521 tttccagggc gcggaactcg atcttgaggt tgtcgaggcg aaggcgctcc aggacgcgca
    11581 gggcccggaa gatgtcccgg cgaagcacgg cgtcgttcag gggccagggg gaggctccgg
    11641 gggcgccgtc ctgaagcagg cgcacctctt tttccgccgc ccggcgggag tccagcaggg
    11701 cctccaaggt ctgctccccc agggcaatct gggtgcgcca agattccttc ttcttggcta
    11761 tttcttccag gcggtccttt tgggcgtcgg caaaggaaag gctatagggg ggcgggggca
    11821 ggacctgagc cccttccggg gcctggtcca acttggccac ttccttctcc agcctgaggc
    11881 gatccgccag agagaccttg atggagctca gggtgttttt aaatagttcg acgcgctcca
    11941 cgaaatgacc ccgggcatcc cgggacaagc cccacagggc ttccacctgc ccgtcgtc
//
LOCUS       DAOOER010000052        11882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_5940_length_11882_cov_8.078295, whole genome shotgun sequence.
ACCESSION   DAOOER010000052 DAOOER010000000
VERSION     DAOOER010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 11882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11882
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5940_length_11882_cov_8.078295"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="PL162_07210"
     CDS             <1..256
                     /locus_tag="PL162_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQF91841.1"
                     /translation="RLASYLGFALSGVSFLYLIYVFYEKFFTDHTVPGWSSLMSMSLF
                     FHGVILVILGILGEYVGRIYDEARDRPRYIVASTKGFPPR"
     gene            431..2128
                     /locus_tag="PL162_07215"
     CDS             431..2128
                     /locus_tag="PL162_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91842.1"
                     /translation="MIFVLLALGMRLGGIQWDQGCHLHPDERFLSMVADALTLPSSPG
                     EYLDPQRSPMNPYNKGFDLFVYGTLPLSVTKALAVFLGMDGYDRLYLLGRGLAALADV
                     LTLLCVVWFSALIQRRYSLDEGLPLLAAFFYAGAVLPIQSAHFFTVDSFLTFFMFSAF
                     VCAFRSSLAPSPLFTALAGLLGGWALATKLPAALIFPLLGILLALPHGRQRRWLALGG
                     HLALFSLAALVALRIGDPHMFAPGVFSLTPEGRFVENIRTLRQWSRPDVWFPPAFQWI
                     DRAPVIFALENLLRWGVGLPFGLLMLLGGALACRSTWRALRRGEYRPDLLVTGVWVLG
                     FFLFQSVQFSKTLRYFLPLYPFFAILAAAGTLGGVALVPGRARRAALGVLVVAAMVWP
                     TAFLSIYTREHSRVTASRWIYENVPPGDVLTAEYWDDALPLPLGNKNPGIYTILSMRV
                     FDEESPAKEAHMNAVLASADWIVLSSNRGYGSLMRLPGRFPHTGQYYRDLFAGRLGFR
                     REATFTSYPRIDLGLFSWEFPDDGAEEVFSVYDHPQVTIFRRVSRDAGPPGETSPQP"
     gene            2125..3183
                     /locus_tag="PL162_07220"
     CDS             2125..3183
                     /locus_tag="PL162_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HQF91843.1"
                     /translation="MSLQTLLKTLDRPLGGLLGPLARGGARWGGGKKSPFREDLLILR
                     PGGLGDLVCCHLALEYLGRNPRDFFWLVERRSAPWARSHALPHLCYDRDLRAILATFG
                     GRWPRVIDTEQFFGLAQAMALFCRAPGGTVFSFEGNRRSRDSDVTVPYSQNREHESLL
                     FARLFGRALGIAAPLEAPKEIPRRSPGAGYGLLALGGGLAPSRNFPPSFWLSLARAYG
                     DGLPLVISGGRGEGALLEALGAAIPGARILPPSFEAFEEAVRSAERVITVDSGAVHVA
                     SYHGVPTVAVFSSGQERKWAPLGAGSVTLTNPGASCRPCALWGQVPPCPHGLVCRDIP
                     LPQDPFRAPEGKGGTDGR"
     gene            3250..3612
                     /locus_tag="PL162_07225"
     CDS             3250..3612
                     /locus_tag="PL162_07225"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQF91844.1"
                     /translation="MHPALLWGLIFSGIFCGSAGGILLKKAAVEVGQAGSPLAQLLAA
                     CTNPRLVGAVVLYVIPLGFWIYLLRFVPISWLQPIFSLTYVLTPLLALAFLGEPVPPA
                     RWAGIGVIIAGVFLVARS"
     gene            complement(3619..4485)
                     /locus_tag="PL162_07230"
     CDS             complement(3619..4485)
                     /locus_tag="PL162_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA prenyltransferase family protein"
                     /protein_id="HQF91845.1"
                     /translation="MALRDVVTLLRPQQWVKNVFVFAPGFFAFRLGEGEVFAASLGGA
                     LAFGLVASSIYVLNDALDVEADRRHPEKCLRPLAAGTVSMREAAGAAAGCALAGFALG
                     GFLGERVLELLLFYAILNGAYSLRLKHVPLVDVFCVATGFLLRIFVGGAATGIALSSW
                     IIVMTFLLALFIALAKRRDDVLHAAAGRDVRAATAGYNLPLVDMALGVLASVTLVAYL
                     MYTMAPEVGERFGGRPVYLTFPWVLLGILRYFQRTLVFQDSGSPTRIILSDRFLQGVL
                     ALWVLSFFFLGR"
     gene            complement(4509..5480)
                     /gene="galE"
                     /locus_tag="PL162_07235"
     CDS             complement(4509..5480)
                     /gene="galE"
                     /locus_tag="PL162_07235"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HQF91846.1"
                     /translation="MLLVTGGAGYIGSHTVRALQARGYETLVLDNFVKGHRDLVFGPL
                     VEGDLRDEALLDGLFEKYPVDGVVHFAAFSLVGESMADPEAYVTNNVGGTLALLRAMR
                     RAGVARFVLSSTAAVYGEPLQIPIDEEAPTRPTNPYGETKLFIETVCRRYAAAYGLGA
                     IFLRYFNAAGADPEGHTGEHHVPESHLIPLLLDVALGRRASLTVYGSDYPTPDGTCVR
                     DYVHVTDLAEAHVRAVERLEKGGGAAEAFNLGNGRGYSVAQVVEEVRRVTGHPIPVTP
                     GERRPGDPAVLVASSARAEEVLGWRQRYADLSAIVDTAWAWHRRRFS"
     gene            complement(5526..7220)
                     /locus_tag="PL162_07240"
     CDS             complement(5526..7220)
                     /locus_tag="PL162_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91847.1"
                     /translation="MTDGEKKEKRGVGFRGLMAGIMILALGGGAWGWIAQTPYPLGGD
                     FDLFIQRSGLPDTAWQAERADLLVGDGGWTVGLGTLSVDGGGSPPLMIRTTKPSGGVT
                     FRVIKDGDPKRPLTVTVAGEGLESRGVTLLSGDTSRDMVVTWRLAADAPTMVQGSLRA
                     SFDTVSSDMPLALGLLRDPDVFEIGFGVGSAEPVAYRSAFYPAQGMALDVPLVCRPGD
                     QLWVRVVNWGTNTLESSRTGLTFHRLFFGGTPSGAKFPFSSFGMLLKSDSLPTGDTTL
                     TLAVPYAGGNRPSVTYSLGLRVKNYTDLGGGVSADGALWRPMKMILPQELPQLMPVEA
                     VTLSAEAVHPKYTPLVPRAFRAVTLEAPVFTIPPLGKYAALPLGVTWSFSSADLVRSG
                     ILTEPQAEAFFRGVRSGGDVIRTVLSKVGVYKHFSDRALDLRTLVPTVDLPRYFDAGF
                     DGTRVTLVFRVILVDAPEKRVDPISDGGVGWFVIYDGAEDRRFTDPLCLAYTPFAGGI
                     TPTSIPPTAVPTPGPGTPTATPSKAPDLGSGSGCSLGGGAGALLLALPLILLGWRR"
     gene            complement(7239..8003)
                     /locus_tag="PL162_07245"
     CDS             complement(7239..8003)
                     /locus_tag="PL162_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014805950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HQF91848.1"
                     /translation="MRIVRREFEGGGRWGVEGDGPWRPVTFPGLEPLGEEGTGARPGR
                     LLAPAMPSKIVCVGRNYRDHIREMGHDGEPLPTEPGIFLKGPNALADPGAEIPYPPFT
                     RSFHFEGELAVVVGRRLWRVSEEEALKGVLGYTAALDLTARDCQKNDLQWARAKSSDL
                     FCPLGPWIETAVDPDHTVVRTRVNGEVRQEASTELMIFPVGRILSHISSFMTLEPGDV
                     VLTGTPAGVGPLEVGDRVEVTVEGVGTLGVRIVAVS"
     gene            8162..11356
                     /locus_tag="PL162_07250"
     CDS             8162..11356
                     /locus_tag="PL162_07250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQF91849.1"
                     /translation="MSLRKKIFGMLGALLTLSLGAAGWFVGGLVLSHASLLERQDALR
                     RAQSQEIILQRERETLQSIATDWGSRRALQEDAPPEAGTPSSLENGRFQDSLLQNARI
                     DLVLALRRGEGAYFIKTSRRAAGQGAELASDVISRISRLPGGVPPRWSGVLPSSQGPM
                     LMAAAPIVSPEGGGSPQGTLILGRFFRAEEARSLSQSLGLEVAFWPLPSEDPALAPVI
                     SALSSDRNTLAFPPGGHTIHAFMVVRDLFHHPALLLRITVPRTLYQKALHDLRYFVVA
                     VVVGGALLLLAILGIVDLLVVRRLSALERHLAGIADLDHLDTRIPHQGNDELAQVAQE
                     VNRMLEALAEAHRREQAIHQRFQNLVANVPGVVYRSRLDEYWTKVYVSDQFTALTGYP
                     VEDFVDNRRFHMAQIAHPGDSLRVVPEVEECLARGRPYDVRFRIRAADGSWLWINDRG
                     QQFRDSEDGSLWIDGIFTDITDLMASLERIQDSETLHRMVFETSGTALAVWEGRGPLL
                     RVNEEFCRLTGLERSQVEGRRTWESFFDADPFLPGGAPRPQQPPRRYEGRLVDLQGVS
                     HDVAVTVAPFPDGVRHVASLLDVTEARRTRRALEESERRYREMADALPQTLFETDPSG
                     HLIFTNRAGFEAFGFDEDDLRRGLSVFEVIAPEDLPRARENFGKNLGEQDVIPREYLA
                     RRKSGETFPVMVYSQAIRHEGVVEGIRGLVVDITDLKGMEEQLRFLSMHDPLTGLYNR
                     AFFDEEGARMASGRFDPLGVVVADLDGLKLVNDLLGHSAGDRLLRDTGRLLKEAFRSS
                     DVVARVGGDEFAAFLPECGEGFAQEARRRILAAVEHHNETQGGVPLSLSVGCASGPGK
                     SPGLTSLMEEADNLMYQQKRLQSPRSRARLLQRLLESFEQKTPDTSREEALIRTLLSQ
                     KSFPGADPLCGALLARYHDLGYVGLPGEIWRSADLLSEDEWRDMRRHPEIGYRIALAC
                     DHLAPVAEAILGHHERHDGTGYPRGLKGEEILPINRLFAVVDAYNAMLSPRPGRPARS
                     SLEAAEELRRHAGSQFDPEAVAFFLEAFPELEDA"
     gene            11443..>11882
                     /locus_tag="PL162_07255"
     CDS             11443..>11882
                     /locus_tag="PL162_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HQF91850.1"
                     /translation="MKEEPIPSAIGMDLGGHTLGAALVTGTTLKDRAEVPTPEGRSLE
                     AVLEAMEGLARRLAPRGTPVGVGLPGMIDAPRERFLRGPNFPGWSGLPLRRLLQERLG
                     APVRLENDANCYALGEGFAGAARGLRDYVVFTLGTGIGGGVVCAG"
BASE COUNT         1918 a         4315 c         3694 g         1955 t
ORIGIN      
        1 gcgcctggcg agctacctgg gattcgccct ctcgggggtg agctttctct acctcatcta
       61 tgtgttttat gagaagtttt ttaccgacca caccgtcccc ggctggagct ccctcatgag
      121 catgagtctc ttcttccacg gcgtcatcct ggtgatcctg ggaatcctcg gggagtacgt
      181 ggggcgcatc tacgacgagg cccgggaccg cccccggtac atcgtggcct ccaccaaggg
      241 atttcccccc cggtagggga ccgaaggcgc cggaggccct ttgaaagact ccgggggttt
      301 cccccggaga aagaacgccc acgccccggc cggggacccc tcggccgggg agggccgagt
      361 aaggagagat cgatcctcca tgagcccctt ttcgcccctc ctcccctcac cccgccatag
      421 gggaattatt ttgattttcg tgctcctggc cctggggatg cgcctggggg ggatccagtg
      481 ggaccaggga tgccacctcc acccggacga gcgcttcctg tccatggtgg ccgacgccct
      541 gaccctcccc tcctccccgg gggagtacct ggacccccaa cgctccccca tgaaccccta
      601 caacaagggc tttgacctct tcgtgtacgg cacgcttcct ctgtcggtca ccaaggccct
      661 cgccgtcttt ctcggcatgg acggctacga ccgtctgtac ctcctggggc gaggcctggc
      721 ggccctcgcc gacgtcctca ccctcctctg cgtcgtctgg tttagcgccc tgatccagcg
      781 gcggtactcc ctggacgagg gccttcccct gctcgccgcc ttcttctacg ccggggcggt
      841 gctccccatc caatcggccc actttttcac ggtggatagc tttcttacat ttttcatgtt
      901 ttccgccttc gtctgcgcct ttcggagctc cctcgccccg tccccgctct tcaccgccct
      961 ggcggggctc ctgggggggt gggccctggc caccaagctt ccggcggccc tcatcttccc
     1021 cctcctggga atcctcctgg ccctgcccca cggccgccag cgccgatggc tcgccctggg
     1081 ggggcatctg gccctctttt ccctcgccgc cctcgtcgcc ctccgcatcg gcgaccccca
     1141 catgttcgcc ccgggggtct tctccctgac ccccgagggg cgcttcgtgg aaaacatccg
     1201 cacgctgagg cagtggagcc gccccgacgt gtggtttccc ccggcgtttc agtggatcga
     1261 ccgggccccc gtgatctttg ccctggaaaa cctcctgcgc tggggagtgg gactcccctt
     1321 cgggctactt atgctcctgg ggggcgccct cgcgtgccgg agcacctggc gcgccctgag
     1381 gcggggggag taccgccccg acctcctcgt caccggcgtc tgggtgctgg gattttttct
     1441 ctttcagtcc gtccagtttt ccaagaccct gaggtacttt ctgcccctct atcccttctt
     1501 cgccatcctg gccgccgccg gtaccctggg aggggtggcc ctggtccccg gcagggcccg
     1561 gcgggcagcc ctaggggtct tggtcgtcgc cgccatggta tggcccacgg cgtttttatc
     1621 catctacacc cgggagcaca gccgggtgac cgcctcccgg tggatctacg aaaacgtccc
     1681 cccgggcgac gtcctcacgg cggagtactg ggacgacgcc ctccccctcc ccctggggaa
     1741 taaaaacccc gggatctaca ccatcctctc gatgcgggtc ttcgacgaag aatcccccgc
     1801 caaggaggcc cacatgaacg ccgtcctggc ctccgccgac tggatcgtgc tctccagcaa
     1861 ccggggatat gggagtctca tgcgcctccc cgggcgcttt ccccatacgg ggcagtacta
     1921 ccgggatctg ttcgctggac gcctgggctt tcgtcgggag gcaactttta cctcctaccc
     1981 ccgcatcgac ctgggccttt tctcctggga gttccccgac gacggcgccg aggaggtctt
     2041 ttccgtctac gaccaccccc aggtgacgat ctttcgccgg gtctcccggg acgccgggcc
     2101 cccgggggaa acctcgcccc aaccatgagc cttcagacgc tgctaaagac cctggaccgc
     2161 cccctggggg gcctgctggg tcccctcgcc cggggagggg cccggtgggg gggcgggaaa
     2221 aaatctcctt tccgggaaga cctcctgatc ctccgccccg gggggctagg ggatctggtc
     2281 tgttgccatc tggccctgga gtacctggga cgaaaccccc gggatttttt ctggctcgtg
     2341 gagcggcgct ccgccccctg ggcccggagc cacgccctgc cccacctctg ctacgaccgg
     2401 gatctccggg ccatcctcgc caccttcggg ggtcgatggc cccgggtgat cgacaccgag
     2461 cagttcttcg gcctcgccca ggccatggcg ctcttttgcc gcgcccccgg gggaacggtc
     2521 ttctccttcg agggcaaccg gcgttcccgg gattccgacg tcaccgtgcc ctactcccag
     2581 aaccgggagc acgagagcct gctttttgcc cgcctcttcg gccgggccct gggaatcgcc
     2641 gcccccctcg aggctccaaa ggagatcccc cggagatccc ccggggcggg gtacgggcta
     2701 ctggccctgg ggggagggct cgccccctcc cggaactttc ccccgtcctt ttggctctcc
     2761 ctggcccggg cctacggcga cgggcttcct ttggtgatct ccgggggaag gggcgagggg
     2821 gcgctcctgg aggccctggg ggccgcgatc cccggggccc ggatcctgcc cccctccttc
     2881 gaggccttcg aggaagcggt gcgaagcgcc gagcgcgtga tcaccgtgga ttcgggggcg
     2941 gtgcacgtgg cctcctacca cggcgttccc accgtagcgg tcttttccag cgggcaggag
     3001 cgcaaatggg cccccttggg agcgggaagc gtcaccctga ccaaccccgg ggcttcctgc
     3061 cgcccctgcg ccctctgggg acaggttcct ccctgccccc acggcctggt ctgtcgggac
     3121 attcccctcc cccaagaccc ctttcgggct ccggagggaa aagggggaac cgacgggaga
     3181 tgacgccgtg ggggaagagc cccggccgaa agccgggggc aattttttcg tgaaagaaag
     3241 gggacgccca tgcacccagc cttgctctgg ggactgattt tttcgggaat tttctgcgga
     3301 tcggcggggg gaattctcct caaaaaagcc gccgtggagg tgggacaggc cggatctccc
     3361 ctggcccagc tcctggcggc gtgtaccaac ccccgactgg tgggggcggt ggtgctttac
     3421 gtcattcccc tgggcttttg gatctacctg ttgcgcttcg tgcccatcag ctggctccag
     3481 cccatctttt ccctcaccta cgtgctcacg cccctcctgg cgctggcctt tctgggagag
     3541 cccgtccctc cggcgcggtg ggcgggcatc ggcgtgatca tcgccggggt ctttctggtg
     3601 gcccggtcct agctcccttc accgccccaa aaagaagaag gacaggaccc acagggccaa
     3661 tactccttgg aggaaacggt ccgaaaggat gatgcgggtg ggagagcccg aatcctggaa
     3721 caccaaggtg cgctggaagt agcgcaagat ccccaggaga acccagggaa aggtgaggta
     3781 caccgggcgc ccgccgaagc gctcccccac ctcgggcgcc atggtgtaca tgagataggc
     3841 caccaaggtc accgaagcca acacccccaa agccatgtcc accaggggaa ggttgtaccc
     3901 cgccgtggcg gcccggacgt cacgccctgc ggcggcgtgg agcacgtcgt cgcgcctctt
     3961 ggccagggcg atgaatagcg ccagcagaaa ggtcatgacg atgatccacg acgaaagggc
     4021 aatgcccgta gcggcccccc ccacgaagat ccgcaacaaa aatcccgtgg ccacacagaa
     4081 gacgtccacc aggggcacgt gcttgaggcg cagggagtag gcgccgttca ggatggcata
     4141 aaagagcagg agctccaaaa ccctctcccc cagaaaccct cctagggcga agcccgcgag
     4201 ggcacaccct gcggcggccc ccgccgcttc ccgcatggac acggtccccg ccgccagggg
     4261 gcggaggcac ttttccgggt gcctccggtc ggcctccacg tcgagggcgt cgttcaggac
     4321 gtagatggag ctcgccacca ggccgaaggc cagggccccc ccaagggagg cggcgaagac
     4381 ctccccctcc cccaggcgaa aggcaaaaaa acccggagca aagacaaaaa cgttttttac
     4441 ccactgttgg gggcgcagca gcgtcaccac atcccgtagg gccatgccgt cacccccctt
     4501 tcgctttttt aagaaaagcg ccgccggtgc caggcccagg cggtgtccac gatggccgaa
     4561 agatcggcgt aacgctggcg ccaccccaag acctcctccg cccgggcgga agaggccacc
     4621 aatacggcgg gatccccggg gcgacgctcc cccggggtca cggggatggg gtgccccgtc
     4681 actcgacgaa cctcctccac cacctgggcc acggaatatc cccggccgtt tcccaggtta
     4741 aaggcctcgg cggcgcctcc gcccttttcc agccgctcca ccgcccggac gtgggcctcc
     4801 gccagatccg tcacgtggac gtagtccctc acgcaggtgc catcgggggt gggatagtcc
     4861 gagccgtata ccgtgaggga ggcccgtctc cccagggcca cgtccagcag caggggaatg
     4921 aggtgggatt ccggcacatg gtgctccccc gtgtgcccct ccggatcggc cccggcggcg
     4981 ttgaagtagc gcaaaaagat cgcccccagg ccgtaggccg ccgcgtagcg tcggcagacc
     5041 gtctcgatga agagcttggt ctccccgtag gggttggtgg gccgggtggg ggcctcctcg
     5101 tcgatgggga tctggagggg ctccccgtag accgccgccg tggaggaaag cacgaaacgg
     5161 gccacccccg cccgccgcat ggcccggagc agcgccagcg tcccccccac gttgttcgtc
     5221 acgtaggcct cggggtccgc catggactcc cccacgaggc tgaaggcggc aaagtgcacc
     5281 accccgtcca cggggtactt ttcaaagagg ccgtcaagca gggcctcgtc ccgcaggtct
     5341 ccctccacca gagggccaaa gaccagatcc cggtgaccct tgacgaaatt gtccagcacc
     5401 aaagtctcgt acccccgggc ctggagggcc cgcaccgtgt ggctcccgat gtatcccgcg
     5461 cctcccgtca ccaaaagcat ctttatagct cactcccgtc cttgaagttc cggcccccta
     5521 gggcctcacc ggcgccaccc caaaaggatc aggggcagcg ccagaagaag ggcccctgcc
     5581 cctcccccca gcgaacatcc gctccccgat cccagatccg gcgccttgga gggggtcgcc
     5641 gtaggggttc ccggccccgg ggtgggcacg gcggtgggag gaatggacgt gggggtgatg
     5701 ccccccgcaa agggggtata ggccaggcaa agggggtccg taaagcgccg atcctcggcg
     5761 ccgtcgtaga tcacgaacca ccccaccccc ccatccgata tggggtccac cctcttttcc
     5821 ggggcgtcca ccaggatcac cctaaagacc agggtcaccc gggtcccatc gaagcccgcg
     5881 tcgaagtagc gcggcaggtc caccgtgggg acgagggtcc gcaggtccag ggctcgatcg
     5941 gagaagtgct tgtaaacgcc caccttgctc aacaccgtgc ggatcacgtc ccctccgctt
     6001 cggacccccc gaaaaaaggc ctccgcctgg ggctcggtga ggattcccga gcgcaccaga
     6061 tccgccgagg aaaaggacca cgtcaccccc aggggcaggg cggcgtactt gcccaggggg
     6121 ggaatagtaa agactggggc ttccaaagtc accgcccgga aggcccgggg caccagcggg
     6181 gtgtatttgg ggtgcaccgc ctccgccgag agggtcaccg cctccacggg catcagttgg
     6241 ggcagctctt ggggcaggat catcttcatg ggccgccaca gggcgccatc ggcactgacg
     6301 ccccctccca ggtccgtgta gtttttcacg cgcaacccca gggagtaggt caccgaaggg
     6361 cgatttcccc ccgcataggg caccgccaag gtcagggtgg tatcccccgt ggggagggag
     6421 tcgctcttaa gcagcatgcc gaaggaggaa aagggaaact tcgcccccga aggagtgccg
     6481 ccgaagaaga ggcggtggaa cgtcagcccc gtgcgagagc tttccaacgt gttggtgccc
     6541 cagttcacca cccgcaccca gagctgatcc ccggggcggc agaccagggg cacgtcgagg
     6601 gccatgccct gggccggata gaacgccgat cggtaggcca cgggctccgc cgatcccacg
     6661 ccgaagccga tctcgaagac gtcggggtcg cgtagcagcc ccagggctag gggcatgtcc
     6721 gacgagacgg tgtcgaagga ggcccgaagg ctcccctgga ccatcgtcgg ggcgtcggcg
     6781 gcgaggcgcc aggtcacgac catatccctc gaggtgtccc ccgaaaggag cgtcaccccc
     6841 cggctctcca acccctcccc cgccaccgtc acggtgaggg gcctcttagg atccccgtct
     6901 ttgatcaccc gaaaggtgac ccccccgcta ggtttcgtgg tgcggatcat gaggggaggc
     6961 gatccccccc cgtcaacgga gagggtcccc aaccccaccg tccacccccc atcccccacc
     7021 aggagatcgg cacgctccgc ctgccatgcc gtatccggca gcccacttct ttggatgaaa
     7081 aggtcgaaat ctccccctag ggggtagggg gtctgggcga tccaacccca cgccccgccc
     7141 cccaacgcca ggatcatgat ccccgccatc aggcccctaa agcccacgcc ccttttttct
     7201 tttttttccc catcggtcat cgaagcccac tcccctttct agcttaccgc cacgatgcgc
     7261 acccccaggg tccccacgcc ctccacggtc acctccaccc ggtctcccac ctccaggggg
     7321 cccacccccg ccggggtgcc cgtcagcacc acgtcgccgg gctcgagggt catgaaggaa
     7381 gagatgtggc tcaggatgcg ccccacgggg aaaatcatga gctccgtgct cgcctcctgg
     7441 cgcacctcgc cattcacccg ggttcgcacc accgtgtggt ccggatccac ggcggtctcg
     7501 atccagggcc ccagagggca aaaaaggtcg gaagacttgg cccgagccca ctggagatcg
     7561 tttttctggc aatcccgggc ggtgagatcc agcgccgccg tgtaccccag gacgcccttt
     7621 agggcctcct cctcggagac tcgccacagc cggcgcccca ccaccaccgc cagctccccc
     7681 tcgaagtgaa acgaccgggt aaagggggga tagggaatct ccgccccggg atccgccagg
     7741 gcattggggc ccttgagaaa gatcccgggt tccgtcggca ggggttctcc gtcgtgcccc
     7801 atctcccgga tgtgatcccg gtagttcctt cccacgcaga cgatcttaga aggcatcgcc
     7861 ggggcgagga gtcggccggg cctcgccccg gtaccctcct cccccaaggg ctccagcccg
     7921 ggaaaggtca cggggcgcca cgggccgtcc ccctccacgc cccaccggcc ccctccttcg
     7981 aactcccgcc gcacgatccg catgacaccc tccctttctc ccccccccgg gggatcgtcc
     8041 cgatcttccc aaatcgtcgc atcacccttg acagggggcc ccctattatc catcatggag
     8101 cgggggtgcg ccccgggcgc tcgacctctt acctctacct ccaccaagaa gggatatacc
     8161 catgtcgctg agaaaaaaaa tcttcgggat gctgggagcg ctcctgaccc tatctctggg
     8221 ggctgcgggg tggttcgtgg gggggctggt gctttcccac gcctccctcc tggagcgcca
     8281 ggacgccctg cgtcgcgccc agtcccagga aatcattcta caacgagaac gggaaacact
     8341 ccagagcatc gcgacggact gggggtcccg gagggccctc caggaagatg cccccccgga
     8401 ggccgggacc ccttcgtccc ttgagaacgg ccgattccag gactccctgc tccagaacgc
     8461 ccgcatcgac ctggtgctgg ccctccgccg aggcgaaggg gcatatttca tcaaaacatc
     8521 ccgccgcgcc gctggccaag gggccgagct ggcctccgac gtgatctccc ggatttcccg
     8581 ccttcccggc ggagtccccc cccggtggtc cggggtcttg ccctcgtccc agggccccat
     8641 gctgatggcc gccgccccca tcgtctcccc ggaagggggg ggatcgcccc agggaacctt
     8701 gatcctgggg cgcttcttcc gggccgagga agcccgatcc ctctcccagt ccctggggct
     8761 tgaagtggcc ttttggcccc tcccctcgga ggaccccgcc ctggcccccg tgatttctgc
     8821 cctctcctcc gaccgaaata ccctggcctt tcctccgggg gggcatacca tccacgcctt
     8881 catggtggtg cgggaccttt tccaccaccc cgcccttctc ctgcggatca ccgtccctcg
     8941 caccctgtac caaaaggccc tccacgacct tcgctacttc gtcgtcgcgg tggtggtggg
     9001 aggggccctc ctgcttttgg ccatcctggg catcgtggac ctcctggtgg tgcgccgcct
     9061 atcggccctg gaacggcacc tggcgggcat cgccgacctg gaccacctgg acacccgcat
     9121 cccccaccag gggaatgacg agctggccca ggtggcccag gaggtaaacc gcatgctcga
     9181 agcgctggcg gaggcccatc ggcgcgagca ggccatccac cagcgcttcc agaacctggt
     9241 ggccaacgtc cccggggtgg tataccgcag ccgcctcgac gaatactgga ccaaagtcta
     9301 cgtcagcgac cagttcaccg ccctcaccgg ctaccccgtg gaggacttcg tggacaatcg
     9361 gcgctttcac atggcccaga tcgcccaccc cggcgacagc cttcgggtgg tccccgaggt
     9421 ggaagaatgc ctggcccggg ggcgccccta cgacgtccgc tttcgcatcc gcgccgccga
     9481 cggctcctgg ctctggataa acgaccgggg acagcagttc cgcgactccg aggacggcag
     9541 cctgtggatc gatgggatct tcacggacat caccgatctc atggcctccc tggagcggat
     9601 ccaagactct gaaacgctcc accgcatggt ctttgagacc tcgggcaccg cccttgccgt
     9661 gtgggaggga cgagggcccc tgctccgcgt caacgaggag ttctgccgcc tcacggggct
     9721 cgagcgatcc caggtggagg ggcgccggac ctgggagagc ttcttcgacg ccgatccctt
     9781 cctccccgga ggggcccctc gcccccaaca gcccccccgt cgctacgagg ggcgcctcgt
     9841 ggacctccag ggggtttccc acgacgtggc cgtcacggtg gcccccttcc ccgacggcgt
     9901 gcgccacgtg gcctccctcc tggacgtcac agaggcccgc cgcacccgaa gggccctgga
     9961 agagagcgaa aggcgctacc gggagatggc cgacgccctg ccccagaccc tcttcgagac
    10021 ggacccttcg gggcacctta ttttcaccaa ccgggcgggc tttgaggcct ttggcttcga
    10081 cgaagatgac ctgcggcggg gcctctccgt ctttgaagtc atcgccccag aagacctccc
    10141 ccgggcccgg gaaaattttg gaaaaaacct aggagagcag gacgtcattc cccgggagta
    10201 cctggcccgg cgcaagagcg gggaaacctt tcccgtcatg gtctactccc aggccatccg
    10261 tcacgagggc gtggtggaag gcatccgagg gctcgtggtg gacatcacgg atctcaaggg
    10321 catggaggaa cagctccgct tcctgagcat gcacgacccc ctcacggggc tctacaaccg
    10381 ggcctttttc gacgaagagg gggcccgcat ggcctcgggg cgcttcgatc ccctgggggt
    10441 ggtggtggcc gacctggacg gcctcaagct cgtgaacgac cttctggggc acagcgcggg
    10501 ggaccgcctc ctccgggata cggggcgcct cctgaaagag gccttccgct cctcggacgt
    10561 ggtggcccgg gtgggagggg acgagtttgc cgccttcctc cccgagtgcg gcgaaggctt
    10621 cgcccaggag gcccggcgtc gtatcctcgc cgccgtggag caccacaacg agacccaggg
    10681 gggcgtcccc ttgagcctct ccgtggggtg tgcctcgggt ccgggcaagt ccccggggct
    10741 gacctccctg atggaagaag cggataacct catgtaccag caaaagcggc tccagagccc
    10801 ccgatcccgg gcccgcctcc tccagaggct cctggaaagt ttcgagcaga agaccccgga
    10861 cacctcccgg gaggaggccc tgatccgcac cctgctctcc caaaagagct tccccggggc
    10921 cgaccccctc tgcggcgccc tcctggcccg ataccacgac ctggggtacg tggggctccc
    10981 gggggagatc tggcgatccg cagacctcct gtcggaggat gagtggcggg acatgaggcg
    11041 ccacccggag atcgggtacc gcatcgccct cgcctgcgat cacctggctc cggtggcgga
    11101 ggccatcctg gggcaccacg agcgccacga cggcacggga taccctcgag gactgaaagg
    11161 agaggagatc ctccccataa accgcctctt cgccgtggtg gacgcctaca acgccatgct
    11221 ctccccccgc cccggacgcc cggcgcgttc ctccctagaa gcggcggaag agctccggcg
    11281 ccacgccgga agccagttcg accccgaggc ggtggccttc ttcctggagg ccttccccga
    11341 gctggaggat gcttaaaaaa ctttagatcg ccaccggggt ccccccagaa gccccgaagg
    11401 ggccttgggc accccccacc gtcccataag gaggggaaaa ccatgaaaga agaacccatc
    11461 ccgagcgcca tcggcatgga cctagggggg cacaccctgg gggcggcatt ggtcacgggg
    11521 acgaccctga aggaccgggc cgaagtcccc accccggagg gacgctcact ggaggcggtg
    11581 ctggaggcca tggaagggtt ggcccggcgc ctggctcccc ggggcacccc cgtgggggtg
    11641 gggctcccgg ggatgatcga tgcccctcgg gagcgctttc tccggggccc caacttcccc
    11701 gggtggagcg gcctgcccct taggagactc ctgcaggagc gcctcggggc ccccgtccgc
    11761 ctggaaaacg acgccaactg ttacgccctg ggggagggct ttgcgggggc ggcccggggc
    11821 ctccgggact acgtcgtctt caccctgggc acgggcatcg gcggcggcgt ggtctgcgcc
    11881 gg
//
LOCUS       DAOOER010000053        11797 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_5992_length_11797_cov_7.874894, whole genome shotgun sequence.
ACCESSION   DAOOER010000053 DAOOER010000000
VERSION     DAOOER010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 11797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11797
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5992_length_11797_cov_7.874894"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /gene="tgt"
                     /locus_tag="PL162_07260"
     CDS             <1..727
                     /gene="tgt"
                     /locus_tag="PL162_07260"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HQF91851.1"
                     /translation="IQEELGSDIAMCFDECVALPASPERSRDALERTLRWAERCQRAH
                     GRKDQALFGIVQGATFPDQRRECAERMTRMDFPGYGIGGLSVGESHEEMYAMLEVTNA
                     VLPKDKPRYLMGVGLPENLVEGVARGVDMFDCVLPTRNGRNGTVFTLRGRLNLKAQRY
                     LRDEGPLDPTCDCYVCRHHSRAYIRHLYRAGEILAARLCSWHNLHFLIRLMRRLRGAI
                     LQGRFDGLRREVMSALREGDVVP"
     gene            724..1719
                     /locus_tag="PL162_07265"
     CDS             724..1719
                     /locus_tag="PL162_07265"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HQF91852.1"
                     /translation="MRCLKVDPRHPDAESIAEAGDLLRRGGLVAFPTETVYGLGADAL
                     DPRAVRRIFEAKGRPGDNPLILHVPRWEDAEGVARVDPRAAFLFRKFSPGPLTLVLPA
                     LPSVPREVTAGLDTVAVRIPNHAVALALLREAGCPVAAPSANSSGRPSPTTAEAVLED
                     LGDRVDLVLDGGPAAVGLESTVVDATGEHLVLLRPGGLSLEDLEALGGPVCLPDHEVV
                     LRRSPGTRHRHYAPRIPVVLWDPGEGGRAGSEELDPVRRWGYVGMAPPPFPCDMERRF
                     PDLAAYAAGLFAALRSLERGAAEAILAEWPEGVGVGRALRDRLLRASEPSYTSGR"
     gene            1837..1923
                     /locus_tag="PL162_07270"
     tRNA            1837..1923
                     /locus_tag="PL162_07270"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1871..1873,aa:Leu,seq:cag)
     gene            2078..5680
                     /gene="rpoB"
                     /locus_tag="PL162_07275"
     CDS             2078..5680
                     /gene="rpoB"
                     /locus_tag="PL162_07275"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HQF91853.1"
                     /translation="MPVLAPADRRSRRLAFGRPHDLVDLPDLIEVQRDSYRWFFQFDV
                     EASQRRSVGLQELFEEIFPVESYDGSFALEFVSYHTDPPSTSEEEARQRDLTWSMPVR
                     ATIRLVNRKVGEIKEEEVFLGDFPIMTNNGTFIVNGTERVVVNQLARSAGVYYSSDGG
                     VPGQETFAAKVIPDRGAWLEFDLSPGDVLSVNIDNRKKIPITMLLKAFGVPNNDDLLL
                     RFGATEEEVELVEEEVKGLLIAESVANATGHAVVRKNERLTKEHLEELWKLGRSRVRV
                     WRVDRALAATLEKDNSLTVDDAVLELFRRLRPNEPARMENAREYISGLFFDPRRYNLG
                     RVGRYKLNRRLGLSIPESIRLLTVDDMVPIIKGLLGMRDGVGQEDDIDHLGNRRVRAV
                     GELLQNQVRIGLLRMERIAKERMTTIPDLAAATARELINVRPISASLREFFGSGQLSQ
                     FMDQTNPLAELTHRRRLSALGPGGLSRERAGFEARDVHYTHYGRVCPIETPEGPNIGL
                     VTSLATYARVNSYGFLVTPRRRTVGGVVGDEVDYLSADEEDAFYVGRADAAMDPQGKL
                     LGDEVHCRHKDTIVSVGPQKVNFIDVSPKQIVSVSTALIPFLEHDDANRALMGSNMQR
                     QAVPLVRAEAPVVGTGIEGRIAIDSGACVLARRSGTVTYVDANRVEVTSDDGMVQKYV
                     LVKFRRSNQGTIIHQKIVVRRGDRVEMGQVLADGQSVENGELALGRNVVVAFVSWEGY
                     NYEDAILLNERLVKEDYYTSIHIEEYEMEARDTKLGPEDITRDIPNVGEDALRNLGED
                     GVVRVGAEVKSGDILVGKVTPKGESDQSPEEKLLRAIFGEKAREVRDTSLRVPHGEGG
                     KVVLIKRLSREHNAEDLSPGVNEVVKVYVAQLRKITVGDKMAGRHGNKGVVSRILPEE
                     DMPYLPDGTPVDVVLNPLGVPSRMNLGQVLETVMGFVGYCGIEGQGGPFRIATPVFEG
                     AQEEEIFSLIDELKQERYPELSGDGRITLYDGRTGEPMERKVTVGCMYMLKLIHLVDD
                     KIHARSIGPYSLITQQPLGGKAQFGGQRFGEMEVWALEGYGASFILQEMLTVKSDDIR
                     GRLKTYERIVKGQNLSEPGVPESFRVLVKELQGLSLDVEIMYDDGSVGGLTLEDDEYD
                     RSRIGSLASFRSDVAVHDDEEEVTERGSPSSDTESFLFGGRTGDKTGAEGETQR"
     gene            5677..>7361
                     /locus_tag="PL162_07280"
     CDS             5677..>7361
                     /locus_tag="PL162_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HQF91854.1"
                     /translation="MSRREIAGVRIKLSSPERIRELSSGEVKKPETINYRTLRPEKDG
                     LFCERIFGPTRSFECACGKYKRSGPKFKGITCDRCGVTVTDNRVRRERMGHIELAAPV
                     VHIWYLRGIPSRLSLLLGTATKDLERVVYFAPSRRRETVFKVVVDGKRHDLVKKGDTI
                     LACEEAIHRHYDPKFNAEGAYRIMTVDDVPLAEGDVVGAHMISRYRAEYGEDLFKVEP
                     AYRCVEDASGLAAGEILGATRLAELRSTGEIVVERVLVGNQEGFVVTATARLPFAKND
                     IVSDSEYKLFHEKYPGRFSAQMETRTLEDPCYIVKEQGSSPFERGFIILESEYRLCAA
                     YDKKFSAAIGAEGVQDLLGVLNMDELAEELREEIAESSGQKKRKLVKRLNVVEDFRKS
                     DSKPEWMVLNVLPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKKLQELKAP
                     EIIIRNEKRMLQESVDALIDNGRVGKAVLGAGNRPLKSLSDLLRGKKGRFRQNLLGKR
                     VDYSGRSVIVIGPHLKIYQCGLPKQMALELFKPFVMHKLVERGMAPNVKSARRL"
     assembly_gap    7362..7461
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7462..>9364
                     /locus_tag="PL162_07285"
     CDS             <7462..>9364
                     /locus_tag="PL162_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HQF91855.1"
                     /translation="SPASGKPIVTPSQDIVLGIFLLTQLREDLRQERLYHFRDLDDCN
                     TALHHGVVHESTPVMLRWEGEWIETSPGRALFNSVLPPKLRFINKAVDKKALSKLLDD
                     AFDQVSPVQLVATLDAIKSLGYTWSTRSGVSFGLDYVVVPPQKKEVVDATMVQEGEVS
                     SQYQMGILTEEEYLRQKELLWSNASREVAERIVQNMAEHNHLRMMVDSGARGTKSQVA
                     QMAGIRGLMADPSGRIIDYPIVANFREGLNMLEYFISTHGARKGLADTALRTAKSGYL
                     TRRLVDVAQDLIITEEDCGTEKGICIEPLLQDGNVVISLSDRVSGRTALRDLYHPSTG
                     EILVKSGEEITPECAKLLDRLEFHQVWVRSPMTCILRNGVCRTCYGRDLATRKKVSIG
                     EAVGVVAAQSIGEPGTQLTMRTFHTGGVRLTGEDITQGLPRIEQLFEVRRPKKVAFLS
                     KTEGTVVEIRETEGKRKVIVASREGEKEQRVQYNVPISQNLLVEEGDPVQRGQELTEG
                     HKDPQDLLECLGVEEVQKYLVDAIQEVYRSQGVPINNKHIEVILRKVAPINRVRIVEE
                     GDSSFVASELVWADDLEKDMVQVRADNSAYLEEAHRALEGMVLRDMVTTGNAEAVNPY
                     KGEELTPEK"
     assembly_gap    9365..9464
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9465..10300
                     /locus_tag="PL162_07290"
     CDS             <9465..10300
                     /locus_tag="PL162_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HQF91856.1"
                     /translation="EDVAYLAEDVSLEGAVLVAKDTLLDRSAVASLQRAPVESIKVWK
                     NTERLVVTELMREHLIKNVWGHPLGRVLGKDGTEINDALPPIVDGAIVRGLLEGSLTA
                     IEIKGEILSADRVLRAVLQDRAYGKVILEDVPGRKGQVAAHSGQLVDQDVLEKLVRAF
                     PEQLHLRTVHATAEKCPLIQQVTFVRRLREEPQARPLLHGITKAALATDSFLSAASFQ
                     QTAQVLAAAAVRGEVDPLRGLKENVIIGHLVPAGTGVDRFRRIEVRRTDGDRGEPSSE
                     A"
     gene            10489..10764
                     /locus_tag="PL162_07295"
     CDS             10489..10764
                     /locus_tag="PL162_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HQF91857.1"
                     /translation="MPLSELAVSHRVVGVRQVWQKLRKASLQKLFVAEDAEARIVSGL
                     VEEAEKLGIAVERVASMKTLGRACAIDRGAAAAGISQNEDGHLRRPL"
     gene            10795..11175
                     /gene="rpsL"
                     /locus_tag="PL162_07300"
     CDS             10795..11175
                     /gene="rpsL"
                     /locus_tag="PL162_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HQF91858.1"
                     /translation="MPTISQLIRLGREEKRQTSGAPALQQNPQRRGVCTRVYTVTPKK
                     PNSALRKVARVRLTNSIEITAYIPGEGHNLQEHSVVLVRGGRVKDLPGVRYHIIRGTL
                     DCGGVENRKQSRSKYGARRGKKKK"
     gene            11250..11720
                     /gene="rpsG"
                     /locus_tag="PL162_07305"
     CDS             11250..11720
                     /gene="rpsG"
                     /locus_tag="PL162_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HQF91859.1"
                     /translation="MPRKGHVRKREGKPDAVFGSLVVGKFINCLMWDGKKSTAERIFY
                     GALERAAKRVSAEPLEVFTKAMENVRPLVEVRPRRVGGATYQVPVEVQGPRAQALATR
                     WVIGFARSRKGIPMVERLARELCDAFKNEGGAVKKREDTHRMAEANRAFAHYRW"
BASE COUNT         2326 a         3333 c         3818 g         2120 t
ORIGIN      
        1 catccaggag gagctgggga gtgatatcgc catgtgcttc gacgaatgcg tggccctccc
       61 cgcgtctccg gaacgctccc gggatgccct ggaacgtacc ctgcggtggg ccgagcgctg
      121 ccagagggcc catgggagga aagaccaggc cctcttcggc atcgtccagg gggcaacttt
      181 tccggatcag cgacgggagt gcgccgagcg catgacccgc atggactttc cgggatacgg
      241 tatcggcgga ctctctgtgg gggagagcca cgaggagatg tatgccatgt tggaggtgac
      301 gaacgccgtc ctgcccaagg acaagccccg ctatctcatg ggggtggggt tgccggagaa
      361 cctggtggag ggggtggccc ggggggtgga catgttcgac tgtgtgctac ccacccgcaa
      421 cggacgcaac ggcacggttt ttacgctgcg gggaaggttg aacctgaagg cccagcgtta
      481 tctccgggac gagggcccct tggaccccac ctgtgactgt tacgtctgtc gccaccactc
      541 ccgggcctat atacgtcacc tgtaccgggc gggggagatt ttggccgccc gtctctgctc
      601 ctggcataac ctgcactttc tgatccgcct catgcggcgc ctccgaggag ccatcctgca
      661 gggccgtttc gacggactgc ggcgggaggt catgtcggcg ctacgggaag gagatgtggt
      721 cccttgaggt gtctgaaggt ggacccccgg caccccgacg cagaatctat cgccgaggcg
      781 ggggacctgt tacgccgagg ggggctcgtg gcctttccca cggagacggt gtacggcctg
      841 ggggcggacg ctttggatcc ccgggcggtg cggcggatct tcgaagccaa gggacgtccg
      901 ggagataacc ccctgatcct ccacgtaccc cgttgggaag acgccgaggg cgtcgcccgg
      961 gtggatcccc gggcggcatt cctttttcgc aagttttctc cggggcccct gaccctcgtt
     1021 ctcccggccc ttccttccgt gccccgggaa gtcactgcgg ggcttgatac ggtggcggtt
     1081 cgcatcccga accacgccgt ggcgctggcc ttgctccgag aggcggggtg ccccgtggcg
     1141 gcccccagcg ccaactccag cggccgcccc agccccacca cggccgaagc cgtactggaa
     1201 gacctggggg atcgggtgga cctcgtgttg gacggaggac ccgccgccgt gggactggaa
     1261 tccaccgtgg tggacgccac gggggagcat ttggtgctct tgcgccccgg gggcctttcc
     1321 ctggaggacc tggaggccct gggagggccg gtgtgccttc cggatcacga ggtagtcctg
     1381 cggcgttccc ccggcacccg ccaccggcat tacgcccctc ggataccggt ggtcctgtgg
     1441 gatcccgggg aaggggggcg tgccggatca gaggagctcg atcccgtccg tcgatggggg
     1501 tatgtgggca tggctccccc gcccttcccc tgcgatatgg agcgtcgctt tcccgacctg
     1561 gcggcctatg cggcggggct ctttgccgcc ttgaggtcct tggagcgcgg cgccgccgag
     1621 gccatcctcg ccgagtggcc ggaaggcgtc ggcgtggggc gcgccctgag ggatcgcctc
     1681 ctccgggctt cggagccctc ttatacttcc gggcgatagc cgcgctgatc ccaggggttt
     1741 ctcctagccg agaccacatt tttcgaatcg aggtggtccc ggggctcttg ccaaatctcc
     1801 cccgggttgc tacaatgctc tccgttacga aaaaacgtcg gggtggcgga attggtagac
     1861 gcgctagatt caggttctag tgggctgacg cctgtgggag ttcgagtctc cccctcgaca
     1921 ccatcgtacg gagcgccttt ctctgggagg gcggaaagaa aaggcagata ggggtacttg
     1981 tgtgcagttg acacgagtac ccccttctgt tatgatttac cttcgtcaca tccccatttc
     2041 cccacatcct tttctcgata gggaggttgc agctcccatg cccgtattgg caccggcgga
     2101 caggaggtca aggcggctgg cattcggtcg accccacgat ctggtggatc ttccggatct
     2161 tattgaggtg cagcgcgact cgtatcggtg gtttttccag ttcgatgtcg aagcctccca
     2221 acgccgtagc gtggggcttc aagagctctt cgaagaaatt ttccccgtgg aaagttatga
     2281 cggatccttt gccctggagt tcgtctccta ccacaccgat ccgcccagca cctccgaaga
     2341 ggaggctcgg cagagggacc ttacgtggtc catgcccgtc cgggccacca tccggttggt
     2401 caatcggaag gtgggcgaga tcaaagaaga agaggttttc ttgggcgatt tccccatcat
     2461 gaccaataat ggtacattca ttgtaaatgg caccgaacgg gtggtggtga accagttagc
     2521 ccgttccgcc ggagtctact acagctccga cgggggagtg ccggggcagg agacctttgc
     2581 ggcgaaggtc atccccgacc ggggagcttg gctggagttc gatctgtccc ccggggacgt
     2641 gctttccgtc aacatcgaca accgcaagaa gatccccatc accatgcttc tcaaggcctt
     2701 tggtgtgccg aataacgatg atttgctcct gcgcttcggg gccacggagg aggaggtgga
     2761 gctcgtcgaa gaagaggtca aggggctcct catcgccgag agcgtggcca atgccacggg
     2821 acacgcggtg gtccgcaaga atgagcgcct caccaaggag cacctggaag agttgtggaa
     2881 gttggggcgg tcccgcgttc gagtctggcg ggtcgatcgg gccctggcgg cgacgttgga
     2941 aaaagacaac tccctcacgg tggatgacgc cgtgctagag ctcttccggc gccttcgccc
     3001 caacgagccc gcccgcatgg agaacgcccg agagtatatt tcggggcttt tcttcgaccc
     3061 caggcgctat aacctggggc gtgtggggcg atataagctc aaccgcaggc tggggttatc
     3121 catccctgaa tccatccgtc tcctcacggt ggacgacatg gttcccatca tcaagggtct
     3181 cctgggcatg cgcgacggcg tgggccagga agacgacatc gatcacctgg gaaaccgccg
     3241 ggtgcgtgcc gtgggagagc tactgcagaa tcaagtccgc atcggcctgc tccgcatgga
     3301 gcgcatcgcc aaggagcgca tgaccaccat ccctgacctg gcggcggcca cggcccggga
     3361 gctcatcaac gtgcgcccca tttcggcctc gttgcgggag tttttcggat cggggcagct
     3421 ttcccagttc atggaccaga ccaacccctt ggcggagctc acccatcgtc gccgcctgtc
     3481 cgctctgggt cccgggggcc tttctcggga acgggccggg tttgaagccc gggacgtgca
     3541 ctatacccat tacggccggg tgtgccccat cgaaacccct gaagggccga acatcggcct
     3601 ggtgacgtct ttggccacct acgcccgggt taattcctac ggatttctgg tgacgccgcg
     3661 gcgccgtacg gtgggtggag tggtggggga cgaggtggat tacctctcgg cggacgaaga
     3721 agacgccttc tacgtaggac gggccgatgc ggcgatggac ccccagggga aactcctggg
     3781 agacgaggtt cactgtcgcc acaaggatac catcgtctcc gtaggaccac aaaaggttaa
     3841 ttttatagac gtttctccaa aacagatcgt ctccgtatcc acagccctca tccccttcct
     3901 ggagcatgac gacgccaacc gggccctcat ggggtccaac atgcagcgcc aggcggtgcc
     3961 tttggtgcgt gccgaggccc ccgtggtggg gacgggcatc gaagggcgga tcgccataga
     4021 ctccggggcc tgtgtcctgg cccgccgcag tggcactgtg acctatgtgg acgcgaatcg
     4081 tgtggaagtt acctccgatg atggcatggt tcaaaagtat gtattagtaa aatttcgtcg
     4141 atccaaccaa ggaaccatta tccaccagaa gattgtggtg cgtcggggag atcgggtgga
     4201 gatggggcag gtcttggccg acggtcagtc cgtggaaaat ggggagctgg ccctgggacg
     4261 caacgtggtg gtggccttcg tctcctggga ggggtacaac tacgaggacg ccatcctcct
     4321 caacgagcgc ctggtgaaag aggactacta cacttccatc cacatcgaag agtacgaaat
     4381 ggaggcccgg gacaccaagt tgggccccga agacatcacc cgggacatcc ccaacgtggg
     4441 agaggacgcc ttgaggaact tgggtgagga cggcgtggtg cgggtcggtg ccgaggtcaa
     4501 atccggcgat atcctggtgg gaaaggtcac ccccaagggg gagtcggatc agtctccgga
     4561 ggaaaaactc ctccgggcca tttttggcga aaaagcccgg gaggtgcggg acacgagcct
     4621 ccgagtgccc cacggcgaag ggggtaaggt ggtgctgatc aagcgccttt cccgggagca
     4681 taacgccgag gacctgagcc ccggagtgaa cgaagtggtc aaggtctacg tggcgcagtt
     4741 gcgcaagatc accgtggggg acaagatggc cgggcgccac ggcaacaagg gcgtggtgtc
     4801 tcggatcctt cccgaagagg acatgcccta tcttcccgac ggcacgccag tggacgtggt
     4861 gttaaatccc ttgggggtgc cgagccggat gaacctggga caggtgctgg aaaccgtcat
     4921 gggcttcgtg gggtactgtg ggatcgaagg ccaggggggg ccttttcgca tcgccacccc
     4981 ggtctttgaa ggagcccagg aggaggagat cttctccctc atcgatgagc tcaagcagga
     5041 gcgctacccg gagctcagcg gtgacggccg catcaccctg tacgacggcc gtacggggga
     5101 gcccatggag agaaaagtca cggtgggatg catgtacatg ctcaaactca tccacctggt
     5161 ggacgacaag atccatgccc gatccatagg cccctatagc ctcatcaccc aacaacccct
     5221 gggagggaag gcccagttcg gagggcagcg tttcggggag atggaagtct gggccctgga
     5281 gggctatgga gcctctttta ttctgcagga gatgcttacg gtgaagtccg acgacatccg
     5341 tgggcgccta aaaacctacg aacgcatcgt caaggggcaa aacctttctg agcccggagt
     5401 gccggagagc tttcgcgtgt tggtgaaaga gctccagggg ctcagcctgg acgtggagat
     5461 catgtacgac gacggctccg tggggggcct caccctggag gacgacgaat acgaccgctc
     5521 ccggatcgga tccttggcat ccttccgcag cgatgtggcg gtgcatgatg acgaagaaga
     5581 agtcactgaa agaggaagcc cttcttcaga cacggaatcc ttcctcttcg gcggtagaac
     5641 gggcgataag accggggcgg agggggaaac gcagcgatga gtcggcgtga gatcgcggga
     5701 gttcgcatca aactttcgag tccggaacgg attcgggagc tctccagcgg cgaagtgaaa
     5761 aaaccggaga ccatcaacta tcgcactctg cgcccggaaa aggacggcct cttctgcgag
     5821 aggatcttcg gccctaccag gagctttgag tgcgcctgtg gtaagtacaa gcgcagtggc
     5881 cccaagttca aggggatcac ctgcgaccgt tgtggcgtca ccgtgacgga taaccgagtg
     5941 cggcgggaac gcatgggcca cattgagctg gcggcccccg tggttcacat ctggtacctc
     6001 cggggcattc ccagtcgcct gtcgctcctt ttgggcaccg ccaccaagga cctggagagg
     6061 gtcgtctatt tcgcccccag tcgtcgtcgg gagaccgtct tcaaggtagt ggtggacggt
     6121 aagagacatg atctcgttaa aaaaggagat acgatcctgg cctgcgagga ggccatccac
     6181 cgtcactatg accccaagtt caacgccgaa ggggcgtatc gcatcatgac ggtggacgac
     6241 gtccccctgg ccgaggggga cgtcgtgggg gctcacatga tttcccgata ccgggcggag
     6301 tacggggaag accttttcaa ggtggaaccg gcctatcgct gtgtggagga cgccagcggc
     6361 ctggcggcgg gagagatcct gggggccact cgcctggcgg agctccgatc cacgggagag
     6421 atcgtggtgg agcgagtcct ggtggggaac caggaagggt tcgtcgtgac ggcgacagcc
     6481 cgcctgccct ttgccaaaaa cgacatcgtc tctgacagtg aatacaagct cttccatgag
     6541 aaatatcccg gccggttttc cgcccagatg gaaacccgga ccctcgagga tccctgctac
     6601 atcgtcaagg aacaggggag ttctcccttc gaacggggtt tcatcatcct ggagagcgaa
     6661 tatcgcctct gcgcggccta tgacaagaaa ttttccgccg ccatcggcgc cgagggggtg
     6721 caagacctcc tgggcgtgtt gaacatggat gagctcgcgg aagagctccg agaagagatc
     6781 gccgagtcct cgggacagaa aaaacgcaag ctcgtcaagc gtctcaacgt ggtggaggac
     6841 ttccgcaaga gcgatagtaa gcccgagtgg atggtcctca acgtgttgcc cgtcatccct
     6901 ccggacctgc gccccatggt acagctcgac gggggacggt ttgccacgtc ggatcttaac
     6961 gacctctatc gccgggtgat taaccgcaac aaccgcctca agaagctcca ggaactcaag
     7021 gcgccggaga tcatcattag gaacgaaaag cgcatgctcc aggagtccgt ggacgccctc
     7081 atcgacaacg gtcgggtggg caaggccgtg ctgggggcgg gaaaccgtcc cctaaagagc
     7141 ctgtccgatc tccttcgggg aaagaagggg cgcttccgcc agaatctgct gggcaagcgc
     7201 gtggactact ccggacggtc ggtcatcgtc atcggccccc acctgaaaat ctaccagtgt
     7261 ggcctgccta aacagatggc gctggagctg tttaaaccct tcgttatgca taagctcgtg
     7321 gagcggggca tggcccccaa cgtcaaaagc gcccggcggc tnnnnnnnnn nnnnnnnnnn
     7381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7441 nnnnnnnnnn nnnnnnnnnn ntgtcgccag cgagcggcaa acccatagtc accccgtctc
     7501 aggacatcgt gctggggatc tttttactca cccagcttag agaagacttg cgtcaggaac
     7561 gcctgtatca cttccgagat cttgacgact gcaacaccgc cctgcaccac ggggtcgtcc
     7621 acgaaagcac cccggtgatg ctccggtggg agggggagtg gatcgaaacg agcccggggc
     7681 gggcgctctt taacagcgtc ctgccgccta aactacggtt tatcaataag gcggtggaca
     7741 agaaggccct gtccaaactc ttggatgacg ccttcgatca agtttccccc gtccagctcg
     7801 tggcgacctt ggacgccatc aagtccctgg gatatacctg gtcgacgcgg agcggcgtga
     7861 gtttcggcct ggactacgtg gtggtaccgc cccagaagaa agaagtggtg gacgccacca
     7921 tggtgcaaga gggagaagta tcctctcagt accagatggg tatcctgaca gaggaagagt
     7981 atctccgtca gaaggagctc ttgtggtcca atgccagccg ggaagtggcg gagcgcatag
     8041 ttcagaacat ggccgagcac aaccacctcc gcatgatggt ggattctggg gcccggggta
     8101 ccaagagcca ggtggcccag atggcgggca tccggggact catggcggac ccctcggggc
     8161 gcatcatcga ctaccccatc gtggccaact tccgcgaggg gctgaacatg ctggagtact
     8221 tcatctcgac ccacggagct cgcaagggat tggcggacac ggcccttcgt acggccaagt
     8281 ccggatacct cacgcgccgc ctggtggacg tggcccaaga ccttattatc accgaagagg
     8341 actgcggcac cgaaaaaggc atctgcatcg aacctctgct ccaggatggc aacgtggtga
     8401 tttccctttc cgaccgggtg tcggggcgca cggcgctgag agacctctac cacccctcca
     8461 caggagaaat cttggtgaaa tcaggagaag aaatcacccc ggagtgcgcc aagctcctcg
     8521 accgtttgga gttccaccag gtctgggtcc ggagccccat gacctgtatc ctgagaaacg
     8581 gcgtttgccg cacctgttat ggccgagacc tggccaccag gaagaaggtc tccatcggag
     8641 aggccgtggg agtggtggcg gctcagtcca taggcgaacc cggaactcag ctcaccatgc
     8701 ggaccttcca cacgggggga gtgcgcctca cgggagaaga catcacccaa ggccttcccc
     8761 ggattgagca gctcttcgaa gtgcgccgcc ccaagaaagt ggccttcctg tccaagacgg
     8821 aagggaccgt ggtggagatt cgagagaccg agggaaagcg caaggtcatc gtggcctccc
     8881 gcgaaggaga aaaagaacag cgagtccagt ataacgtgcc catctcccag aatcttctgg
     8941 tggaagaagg ggacccggta cagcggggac aggagctgac cgaggggcac aaagaccccc
     9001 aggacctcct ggagtgcttg ggcgtggagg aagtacaaaa gtacctggtg gatgccatcc
     9061 aagaggtcta ccgatcgcag ggggtgccca tcaacaacaa gcacatcgaa gtcatcctgc
     9121 gcaaggtggc tcccatcaat cgggtgcgca tcgtggaaga aggggacagc tccttcgtgg
     9181 ccagcgagct ggtctgggcg gatgacctgg aaaaggacat ggtccaggtt cgcgccgata
     9241 acagcgccta tctggaagag gcccaccgag ccctggaggg tatggtcctt cgggatatgg
     9301 taaccacggg aaatgcagag gccgtgaacc cctacaaggg ggaagagctt acgccggaga
     9361 aaatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntggaag acgtggcgta
     9481 cctggcggag gacgtgtcgc tggaaggcgc cgtcctcgtc gccaaagaca ccctgctgga
     9541 tcgatcggcg gtggcctccc tccagagagc cccggtggaa agcatcaaag tgtggaaaaa
     9601 caccgagcgc ctcgtcgtca cggagctcat gcgggaacac ctcatcaaga acgtgtgggg
     9661 gcaccccttg ggtcgagtcc tgggaaaaga tggtacagaa attaacgacg ccctgcctcc
     9721 catcgtggac ggcgccatcg tccggggtct ccttgagggc agcctgaccg ccatagaaat
     9781 taagggagaa atcctgtcgg cggaccgggt gcttcgggcg gtgttgcagg atcgggccta
     9841 tgggaaggtt atcctggagg atgtgccggg gcgtaagggc caggtggcgg cccacagcgg
     9901 tcaactggtg gaccaagacg tcctggaaaa gctggtgcga gcgttcccgg agcaattgca
     9961 ccttcggacc gtccacgcca cggcggagaa gtgccctctg atccagcagg tgacctttgt
    10021 gcggcgccta agggaggaac cccaggcccg ccccctgctc cacggcatca ccaaggccgc
    10081 cctggccacg gacagcttcc tttcggctgc gtcgttccag cagaccgccc aggtcttggc
    10141 ggcggcggcc gtgcggggtg aggtcgaccc cctgcggggg ctcaaggaaa acgtgatcat
    10201 cggccatctg gtgcccgccg ggacgggggt ggatcgcttc cggaggatcg aagtccgccg
    10261 gacagatgga gacagaggag agccctcttc cgaggcatag tgaacaaatt gaaccatggg
    10321 cagagaaaac ccccaagttt ttagctcccc catcgacctg tgatgggggg gcgggggggc
    10381 ccataggacg aaggggtctt ttggagagac cggagtcagg ggttttccct tgacagcggt
    10441 ctctcctgtt gttatcattc cctagtctgc gtgcgggagg gattctgtgt gcctttatcc
    10501 gagcttgcgg tgtcccatcg agtagtgggg gtacgtcagg tgtggcagaa gctccgcaag
    10561 gcgtcgctgc aaaaactttt tgtcgcggaa gatgcggagg ccaggatcgt ttccggcttg
    10621 gtcgaagagg cagaaaagct gggaatcgcg gtggagcggg tggcttccat gaagaccctc
    10681 ggtcgggctt gtgccataga tcgaggagcg gcggcggcgg ggatcagtca gaacgaggac
    10741 ggacatcttc ggcgaccgct ttaggtcgct gtcatatttg aaggagggag ttttttgcca
    10801 acaatcagcc agcttatccg cctgggacgg gaagaaaaac gccagaccag tggagccccg
    10861 gcgctgcagc agaatcctca gcgccgcggg gtgtgcaccc gggtgtacac ggtgacgcca
    10921 aaaaagccca actcggctct gcggaaggtc gcccgagtcc ggcttaccaa cagcatcgaa
    10981 atcacggcct atattcccgg cgaagggcat aacttgcagg agcactccgt ggtgctcgtg
    11041 cgcgggggcc gagtgaagga cttgcccgga gtgcggtatc acatcattcg cggcaccttg
    11101 gattgcggcg gtgtggagaa tcgcaagcag tctcgctcca agtacggcgc acggcgcggc
    11161 aagaagaaga agtaggggct gcggcagggg cgccagtccg gcgggccatg gaagcatact
    11221 gggcgttcta gaagaaggag gaagttcctg tgccgagaaa agggcatgtg cggaagcggg
    11281 agggaaagcc cgacgccgtc tttgggagtc tcgttgtggg caagttcatc aattgtctca
    11341 tgtgggacgg taaaaagagc acggcggagc gcatttttta cggggcgctg gagagggcgg
    11401 ccaaacgggt gagtgcggag cccttggagg tctttaccaa ggctatggag aacgtgcgtc
    11461 ccctagtgga agttcgtccc cgtcgggtgg gaggagccac ttatcaggtg ccggtagaag
    11521 tccaaggccc ccgggcccag gcgctggcta ctcgctgggt cataggcttt gcccggtctc
    11581 ggaagggtat ccccatggtt gaaagactgg cgcgggagct ctgcgatgcc ttcaaaaacg
    11641 aaggcggcgc ggtgaaaaag cgcgaggaca cccatcgcat ggccgaggcg aaccgggcct
    11701 ttgcccatta ccgctggtag tctcgaaggc tttcgtcggg ttcatcttcc gatggtgaaa
    11761 ggcagataat ggacgtcgta gaccttaaaa atatacg
//
LOCUS       DAOOER010000054        11481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_6175_length_11481_cov_7.769911, whole genome shotgun sequence.
ACCESSION   DAOOER010000054 DAOOER010000000
VERSION     DAOOER010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 11481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11481
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6175_length_11481_cov_7.769911"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..1691
                     /gene="hutH"
                     /locus_tag="PL162_07310"
     CDS             159..1691
                     /gene="hutH"
                     /locus_tag="PL162_07310"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HQF91860.1"
                     /translation="MQAPVLELNGHSLTIEDVVDVARHFRRVALDPAAVTFVDRGADL
                     VASWAREGKVVYGITTGFGDLSHVVIPPDKRELLQRNLLVSHSCGMGEIFPEDVVRAA
                     MLLRINTLIRGFSGITLGTLSRLLHYLNLGIHPVIPCQGSVGASGDLCPLSHMAITLL
                     GLGEVIFRGRRMPTMEALQMEGLEPIALHPKEGIALNNGTQALTAVGALALADALSLA
                     KTADVAAALSLEALHGVPYAFDARTHELRPHVGQKLVASNMRRLIEDSEIVEKYRHQR
                     VQDAYSLRCVPQVHGASRDALEYVRQKVAVEINAVTDNPLIFPADGEALSGGNFHGQP
                     MALAMDFFGIAVAELGNISERRQARMVDGKLSGLPPFLVEESGVNSGFMIPQYTSAAL
                     VSENKVLAHPASVDSIPTSANQEDHVSMGMYASRKGRMILDNVCRVVGIEVMTACQGL
                     EFSKPLRPGRGTRAAYEALRDRVPFVREDAFLSPLMHEALAMVSEGTLLRAVEGAVGD
                     LA"
     gene            1880..2797
                     /gene="ftcD"
                     /locus_tag="PL162_07315"
     CDS             1880..2797
                     /gene="ftcD"
                     /locus_tag="PL162_07315"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HQF91861.1"
                     /translation="MATKLIECVPNYSEGRRPEIIDALVAPFQGRRGCYLFDYRADED
                     HNRLVVSLVGEPEPIQDALIEAAQVALRHIDMNTHVGGHPRVGAVDVTPFVPLRNITM
                     EECAALARDFGQRYWEETKIPVYFYEDAALRPERRRLEVIRKGQYETLKEECLREERR
                     PDVGGPGLHPTAGASVIGARKFLVAFNVNLGTTDARVAKEIANAVRASSGGLCHVKGI
                     GLALEDRGMVQVSMNIVDHEKNAIYRVLEMVRMEARRWGVAVVESEVYGMVPADAVLS
                     SAAYYMQIAGFDPKQIIELRLLEMMGETS"
     gene            2794..4038
                     /gene="hutI"
                     /locus_tag="PL162_07320"
     CDS             2794..4038
                     /gene="hutI"
                     /locus_tag="PL162_07320"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HQF91862.1"
                     /translation="MTVKLFRSVRVVTPQDPGRPLAGPDQGRVADWERGALLVREGRV
                     AAVGSEEEVLREAQGFDVDQEIFGEGRCLVPGFVDPHTHMCFARRREKEFGLRLQGTP
                     YLDILKAGGGILSSVRAVREASDEALYQTTLAHATSALRRGSTTVEIKSGYGLDTATE
                     LRMLQVVRRIARETPLDVVATFMGAHAVPEEYRGRGDDFVALLVEEMIPAVQEQGIAS
                     FCDVFCEEGVFSVPQTRRILEAARRHGLGLKVHADEVHDLGGAELAAELGAISAEHLL
                     AAREEGLRAMAKAGTVAVLLPATAYSLRKPYAAGRTMIDLALPVALATDCNPGSSFTE
                     SMPFVFGLAVLNMYLSPEEALTGATLNAAYAIGQASQVGSLDRGKKADFLLLDGDSPA
                     ILAYHAGVSPVTQVYKMGERVA"
     gene            4228..4857
                     /locus_tag="PL162_07325"
     CDS             4228..4857
                     /locus_tag="PL162_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HQF91863.1"
                     /translation="MELTNLSVRSFLEELASESAAPGGGSVAALGGALGAALAAMVAR
                     LTVGKEKYRDEWEGMERLRDEADALRIRFLDLLSQDANSFNAFMEAMKLPKDTEDQKA
                     KRSKAMQEALLHAARIPLETVRAMGEMARLAREAVARGNRNAVTDAGTAALLARAAAL
                     AAAYNVRANLLALKDQDVAARLRRELEDALAVTLATADEVLPLVEEKLV"
     gene            4857..5750
                     /locus_tag="PL162_07330"
     CDS             4857..5750
                     /locus_tag="PL162_07330"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HQF91864.1"
                     /translation="MREMRGKPLAGALRGWCAHTLGSLGGGRKASLAILQVGDDPAST
                     AYRIQKVKAVDALGGEARVVHLEASASREEAREVLRRWGDDDGVDGIFLEHPLPRGWE
                     DEFRSLIPLRKDVEGLHPDHIGRMYMGDSQVPLPCTAEAAVLLPEWYGWGDFPGKEAV
                     VLGRSVSVGRAVALLLLHRHATVTVLHSRSRSTTEHLSRAEIVVVAAGQRGIVRSHGL
                     RPDAVVVDVGIHVQEDGSLVGDVLWDDDAPRRAFTPVPGGVGPVTVALLLRNLVWSYA
                     AARGTPLAFPSTEELRRYATS"
     gene            5737..6633
                     /locus_tag="PL162_07335"
     CDS             5737..6633
                     /locus_tag="PL162_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91865.1"
                     /translation="MRRADPSRGLRYRGALLLGLLVGALVWGSPAEATPKPERLSIWV
                     TSPWLALMARFLGGVCVEVHPLSGWNDRGQLVRFKRPPAGAWIIALDREEKGLFDLGN
                     VPDARVRLLFSDMPYLRQEGKRNFLDPATMPFLGQEILKALAAFDGDNYRYYQRRLAE
                     FQSRLESTVGVGRQLLSEVPLLNLSLELALWTAAAGKRHVTPSWDMVQAWGRGERMDV
                     FERTLAEAQAGHMVVIYDNQMPPAVKNFLASSSSALLIELPDVNQVDDLFLYLYDQYM
                     AVWNFARHGTAPWRSPGGQRHF"
     gene            6845..7087
                     /locus_tag="PL162_07340"
     CDS             6845..7087
                     /locus_tag="PL162_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HQF91866.1"
                     /translation="MGCSLSQIREELMAHKGACLRYKALKGRRKVEERQGVLVETYPK
                     LFTLFVESQNSTVSFSYAEVLTREVELELIPDPRKG"
     gene            7228..8070
                     /locus_tag="PL162_07345"
     CDS             7228..8070
                     /locus_tag="PL162_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-diphosphocytidyl-2C-methyl-D-erythritol
                     kinase"
                     /protein_id="HQF91867.1"
                     /translation="MEHVVASWGKVNLSLRVTGRREDGYHALHSLFLRVGPLEAMRLR
                     IRSGAGRGDLLHLRSEVPISGENLVLRALALVRQYHKAFPFFEVELQKQIPPGSGLGA
                     GSGNAAAILSWARQQGFPVPDVSAVASLGADVPFLASSWPGGRGEGIGDDLSPFSLPA
                     LRVALVVPRWSCATGLAFAALDRTRGGDWSPLEEARRESDRLLENLAAGKRVGLLPND
                     FGPDLWAAHGEYKDFFDLAEEQGALGWGISGSGGAALALFPRDKALRWPDFSWARMGT
                     ILEV"
     gene            8074..8955
                     /locus_tag="PL162_07350"
     CDS             8074..8955
                     /locus_tag="PL162_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HQF91868.1"
                     /translation="MRPARPERLARLTARLMFAPSRRISLTENAGEFAISKTMVGNDL
                     SLLDDLFQEEGLGSVVVERGRDGGATFRPRLSPERRQEWLKEVAHILETPDRVLPGGL
                     LYYSDLLFSPFWAQRLGFALASSFADSPIDVVMTTEMKGIPLGMFTAHALGVPLAVCR
                     FGNRPSDGSAVVVHYPSASGDVRTLFMGVRMLPKASRVLLVDDVMRGGSTLAGMLQVA
                     KEFEAQVSGIGVFLVAETPQVKRVGHHTALLRFSISTAGIPCVEVQAPPLAFDGAGYV
                     RYNRSIERGRCRPWTSS"
     gene            8940..9122
                     /locus_tag="PL162_07355"
     CDS             8940..9122
                     /locus_tag="PL162_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HQF91869.1"
                     /translation="MDVKLNRNDCIGCGVCVQICPEVFELDEEAGKARVARQGGTPEA
                     VREAVESCPVGCISVG"
     gene            9175..9251
                     /locus_tag="PL162_07360"
     tRNA            9175..9251
                     /locus_tag="PL162_07360"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9209..9211,aa:Ile,seq:cat)
     gene            9340..10134
                     /locus_tag="PL162_07365"
     CDS             9340..10134
                     /locus_tag="PL162_07365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HQF91870.1"
                     /translation="MKTVKDLMTTLEIIAPSRGMEEWDNGGLLLGSPHWPLGRVGVAL
                     DPSEEIVSAAVCRKCSAVVTHHPMTLTGWKALSLETPEGRLVSRALGESVALVAAHTN
                     WDRAEEGVNVALGKALPLSGMHPLCSSREEGLWGDGGWGDLVTPLPVREMAQNVGRLW
                     GLSWGHLFSPDPSLVVSRLAWVGGSGGSFWRRAREKGATLFCTADMKYHERRDAMAAG
                     LALLVVDHGEMESFSLGSLARLIQSHLGQEVEILAEPPSYGSILLF"
     gene            10275..11270
                     /gene="trpS"
                     /locus_tag="PL162_07370"
     CDS             10275..11270
                     /gene="trpS"
                     /locus_tag="PL162_07370"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HQF91871.1"
                     /translation="MRERVFSGMRPTGRLHLGHLAGALTNWVRLQDDYECFYCIVDWH
                     ALMSDYADSRQLAANGQEILLDWLAVGLDPAKSAIFVQSHVAEHAELHLALSMITPLG
                     WLERCPTYKDQIINMKNKDLSNYAFLGYPVLMAADIILYKAHKVPVGEDQSAHLELTR
                     EVVRRFNHFYGDVFPEPDILLTPTPKVPGTDGRKMSKSYGNSLAIADSLEEIWERIRT
                     MLTDPARIRRTDPGDPEKCPVWDLHKVFNADAAEREEVASGCRSAAMGCIECKKKLHG
                     HICTLLEPIQERRRYYETHGSALEEVLHQGAQKARVVAEATMHHVRSAMGLQPPR"
BASE COUNT         2061 a         3304 c         3878 g         2238 t
ORIGIN      
        1 ggtatactat attccataat tccctagata catcagggaa gtgtgggtac tgcggagaag
       61 ataacggcat ggtcctgtaa aaagtcgggg gcgagattgg cgtcccgggc cgtcgcaact
      121 ccgccgctca aaaaatgcgc aaagggaggg gtaaggagat gcaagcaccg gtattggagc
      181 taaacggaca ttccctgacg atcgaagacg tggtggacgt ggcgcgccac tttcgtcggg
      241 tcgccctgga tccggcggcg gtgacctttg tggatcgtgg cgccgacctg gtggcgtcct
      301 gggcccggga aggcaaagtg gtctacggca tcaccacggg ctttggagac ctgtctcacg
      361 tggtaatccc tccggataag cgggaacttc ttcaaagaaa ccttctggta agccattcct
      421 gcggcatggg ggagatcttc ccggaggatg tggtccgggc ggccatgctc ctgcgcatca
      481 acaccctcat ccgtggtttt tccggcatta ccctggggac cctatcccga ttgctccact
      541 acctcaatct tgggattcat ccggtgatcc catgccaggg ttccgtgggt gccagcgggg
      601 acctttgccc cctatcccac atggccatca cccttttggg gctgggggaa gtgatcttcc
      661 gggggcggcg catgcccacc atggaagccc tccagatgga gggactggag cccatcgccc
      721 tgcaccccaa ggaaggcatc gccctcaata acggcaccca ggccctgacc gccgtggggg
      781 ccctggccct ggccgacgcc ctgtctctgg ccaagacggc agacgtggcc gccgcccttt
      841 ccttggaggc ccttcacggc gttccctacg ccttcgacgc ccggacccac gagttgcgcc
      901 cccacgtggg gcaaaagctc gtggcttcga acatgcggcg cctcatcgaa gatagcgaga
      961 tcgtggagaa gtaccgacat cagcgggtgc aggatgccta ttcgcttcgc tgcgtccccc
     1021 aggtccacgg cgccagtcga gacgccctgg agtacgtcag acagaaagtg gcggtggaga
     1081 tcaacgccgt cacggataac cccctgatct tccccgccga cggtgaggcc ctaagcgggg
     1141 ggaatttcca cggccaaccc atggccctcg ccatggattt ctttggcatc gcggtggccg
     1201 aactgggaaa catctccgaa cggcgccagg cccggatggt ggacggcaag ctgtcgggtc
     1261 ttcccccctt cctggtggaa gaaagtggcg ttaatagcgg attcatgatt ccccagtaca
     1321 ccagtgccgc cttggtgtcc gagaacaagg tcctggccca ccccgcatcg gtggattcca
     1381 tccccacttc ggccaaccag gaagaccacg tttccatggg catgtatgct tcgcgcaagg
     1441 ggcggatgat cctggacaat gtctgccgcg tggtgggtat cgaggtgatg acggcctgcc
     1501 aggggttgga attcagtaaa cccctgcgtc cggggagggg aacccgggcg gcctacgaag
     1561 ccttgcggga tcgagtgccc tttgtgcggg aagatgcatt cctttctccc ctcatgcatg
     1621 aagccctggc catggtatcc gaggggaccc tcctgcgggc ggtggagggg gcggtggggg
     1681 atctggcctg aaccaccatt ctggaatctt ttggttcccc gaggtacccc tggtggcgcg
     1741 gagtttccct cggggatggc cctgccgaag gggtcttcgc ccgatggagg agctcccttt
     1801 tcgggaatcg acgatgttag agagcccctc caccggggct tgaccgtgaa atgagttcga
     1861 agactaggag gtcgtaaaaa tggcgacgaa gttgatcgag tgtgtgccca actacagcga
     1921 gggacgtcgg cccgagatca tcgatgccct ggtggccccc tttcaggggc gtcgagggtg
     1981 ctacctcttc gactatcggg cggacgagga ccacaaccgt ctggtggtga gcctcgtggg
     2041 ggagcccgaa cccatccagg atgccctcat tgaggccgcc caggtggcct tgcgccacat
     2101 cgacatgaac acccacgtgg ggggacaccc ccgggtggga gcggtggacg tgaccccctt
     2161 tgttcctttg cgcaacatta ccatggaaga gtgcgcagct ctggcccgcg actttggcca
     2221 gcgctactgg gaggagacga aaatccccgt ctacttctac gaggacgcgg ccctccgacc
     2281 ggagcgacgc cggttggagg tcatccgcaa ggggcagtac gagaccctga aagaagagtg
     2341 cctccgggag gagcggcggc cggatgtggg aggacccgga ctccacccca ccgccggagc
     2401 gtcggtcatc ggcgcccgga aattcctggt ggccttcaac gtcaacctgg gaacgacgga
     2461 tgcgagagtg gccaaagaga tcgccaacgc cgtccgggcc tccagtggag ggctttgcca
     2521 cgtgaagggc attggcctgg ccttggaaga ccggggcatg gtgcaagtca gcatgaacat
     2581 cgtggaccat gagaaaaacg ccatatatcg ggtgctcgag atggtgcgca tggaggcccg
     2641 gcgttggggg gtggctgtgg tggagagcga ggtttacggc atggtgcctg ccgacgccgt
     2701 gctttccagc gcggcgtact acatgcagat cgcgggattc gaccccaagc agatcatcga
     2761 actccgtcta ttggagatga tgggggaaac gtcatgaccg tcaagctctt tcggtcggtg
     2821 cgcgtggtga ccccccaaga cccaggacgc cccctggcgg ggcccgacca gggaagggtg
     2881 gccgactggg agaggggagc tctgctggtg cgagagggcc gagtggcggc ggtgggctcc
     2941 gaagaagagg tactgcgtga agcccaggga ttcgacgtgg accaggaaat cttcggggag
     3001 gggcgatgtc tggtccccgg tttcgtggac ccccataccc acatgtgctt cgcccggcgc
     3061 cgagagaagg aatttggcct tcgcctccag ggaactccat atttggacat cctcaaggcg
     3121 gggggaggta tcctgtcttc tgtccgggcc gtgcgcgagg cctctgacga agccctgtac
     3181 cagactacct tggcccacgc tacttcggcc ctccggcggg gatccaccac agtggagatt
     3241 aaaagtggat atggtttgga cacggccacg gaactacgta tgcttcaggt ggtgcggcgc
     3301 atagcccggg agacgcccct ggatgtggtg gccaccttca tgggggccca tgcggtcccc
     3361 gaggaatacc gggggcgggg ggacgacttc gtggcgctcc tcgtggaaga aatgattcca
     3421 gcggtgcaag aacaaggcat cgcttctttc tgtgatgtct tttgcgagga gggggtcttt
     3481 tccgttcctc aaacccgccg catcttggaa gccgcccgaa ggcacggcct ggggctcaaa
     3541 gtccatgccg acgaggtgca cgacctggga ggagccgagc tggcggccga gcttggcgcc
     3601 atttcggcgg aacacctttt ggcggcccga gaggagggac tgcgggccat ggccaaggcg
     3661 ggcaccgtgg ccgtcttgct tccggcgacc gcctatagcc tccgaaagcc ctatgcggcg
     3721 gggaggacga tgatcgatct ggccttgccg gtggcgctgg ctacggactg taacccggga
     3781 tccagtttca ccgaatccat gccctttgtc tttggcctgg cggtgcttaa catgtacctt
     3841 tctcccgagg aggccctgac gggggccact ctcaacgccg cctacgccat aggccaggcc
     3901 tcccaggtgg gaagcctgga ccgggggaag aaggcggatt tcctcttgct ggacggcgat
     3961 agtcccgcga tcctggccta ccacgcgggg gtttccccgg tgacccaggt gtacaagatg
     4021 ggggagcgcg tggcttaaga cgatgttgcc cttcttcccc tcgaagggcg gaggacgagg
     4081 ggtgcgggac caaatattgc cggtaaaggg cctttttttg tcgcctcctc ggatcggaag
     4141 atcggatcgg agggtgcttg gggataggtc tccctcgccc cgggaagggg caagggggcg
     4201 agtaaaatca gaaggggaga aaaggacatg gagctgacga acctaagcgt gagatctttc
     4261 ctggaagaac tggcgtccga gtcggcggcc ccgggagggg gaagcgtcgc ggccttgggg
     4321 ggagccctgg gggcggcctt ggcggccatg gtggcgcgct tgactgtggg aaaggagaag
     4381 tatcgggacg aatgggaggg catggagcgg ctccgggacg aggcagacgc cttgcggatc
     4441 cgttttttgg atcttttgtc ccaagatgcc aattctttta atgccttcat ggaggccatg
     4501 aaactcccca aagacaccga ggaccagaag gcgaaacgat cgaaggccat gcaagaggcc
     4561 ctgctccatg cggctcgcat ccccttggag accgtccggg ccatggggga gatggcccgc
     4621 ctggcccggg aagcggtggc gcgtggcaac cgcaatgcgg tcaccgatgc ggggacggcg
     4681 gctctcctcg cccgggcggc ggccctggcg gcggcgtaca acgtgcgggc gaaccttctg
     4741 gcgctaaaag accaagatgt ggctgctcgg cttcggcggg aactagagga tgcgctggca
     4801 gttaccctgg ccaccgctga cgaagtgctt cccttggtgg aagaaaagtt agtctgatgc
     4861 gagagatgcg gggaaagccc ctggcggggg cgctaagggg ttggtgcgct cacaccctag
     4921 gatctctagg gggagggcgc aaggcctctt tggctattct tcaggtgggg gacgaccccg
     4981 cctcgacggc ctataggatc caaaaggtga aggccgtaga tgcgttggga ggggaggccc
     5041 gcgtcgtcca tttggaggct tcggcatccc gggaagaggc tcgagaggtg ctacggcgct
     5101 ggggtgacga cgacggggtg gatggcatct tcctggagca cccccttccc cggggatggg
     5161 aggatgagtt ccgatctctc atccccctca ggaaagacgt ggaggggctg catcctgatc
     5221 acattggccg catgtacatg ggggattccc aagtgccctt gccctgcacc gccgaagcgg
     5281 cggtcctcct ccccgagtgg tatggatggg gagattttcc cggaaaagag gccgtggtcc
     5341 tgggccggag cgtctccgtg ggacgggcgg tggccctttt gctgctacac cgccacgcca
     5401 cagttaccgt acttcacagc cgttcccggt ccacgaccga acacctctcc cgagcagaga
     5461 tagtggtggt ggcggcgggg caacggggga tcgtccgaag ccatggcttg cgccccgacg
     5521 cggtggtggt ggacgtgggg attcacgtcc aggaagacgg atctctggtg ggtgacgtcc
     5581 tctgggacga cgacgccccc cggagggcgt tcactccggt tcccggcggg gtggggcccg
     5641 tcacggtggc gcttcttttg agaaacctgg tgtggtccta cgccgccgcc cgagggacgc
     5701 ctttagcgtt cccttccacg gaagaactcc ggcggtatgc gacgagctga cccttcccgg
     5761 ggactccgat atcggggggc gctcctcctg ggacttcttg tgggggcgct ggtgtggggg
     5821 tccccggcgg aggccacccc aaagccggag cgcctctcga tttgggtcac gtcgccttgg
     5881 cttgccctga tggcgcgttt cctggggggg gtgtgcgtgg aagtgcaccc cctttctgga
     5941 tggaatgacc gggggcagtt ggtgcgcttc aaacgccccc ccgccggggc gtggatcatc
     6001 gccctcgatc gagaagaaaa ggggcttttc gacctgggaa acgtccctga tgcccgggtt
     6061 cgccttcttt tttccgatat gccctatctt cgccaggaag gcaagagaaa ttttttggac
     6121 cccgccacca tgcccttcct cggccaggag attttaaagg ccttggcggc cttcgatgga
     6181 gataactatc ggtactacca gcgccgtctg gcggaatttc agtcgaggct ggagagcact
     6241 gtgggggtag ggcgacagct attgtcggaa gttcccttac taaacctatc cctcgagcta
     6301 gcgctctgga cggccgctgc gggaaagcgc cacgtgactc cctcctggga tatggtccag
     6361 gcctgggggc ggggcgagcg catggatgtc tttgagagga ccttggcgga ggctcaggcg
     6421 ggacacatgg tggtgatcta cgacaatcag atgcctccag cggtcaagaa cttcctggcc
     6481 tcttcttctt cggctctgtt gatagagctt cccgacgtta accaggtgga tgatctcttc
     6541 ctctatctct acgaccaata catggcggta tggaactttg ctcgccatgg caccgcccca
     6601 tggcgatccc cgggaggcca gcgtcacttt tgacgtgccc cggcgcggtc cgatggagct
     6661 atgagatatc tttattcgag gcctcgacgg gcccaagggt tttggtaact ttttttgtgg
     6721 cccccaggcc ccatcggatc gaaatcgtcg gggacttctt gacaaagtga aaaaaatacc
     6781 ctttgcgaaa agagggaaag tatgataaaa tctccccgag cttaggaaag cagaggtggg
     6841 cttgatgggg tgcagccttt cgcagattag ggaagaactc atggcgcata agggcgcgtg
     6901 cctgcgttat aaggcgctta aggggcgacg gaaggtggag gagcgccagg gagtgctcgt
     6961 ggagacctat cctaaactct tcaccctctt cgtcgagtcc cagaacagca cggtctcttt
     7021 tagttacgca gaggtgctga ctagggaagt ggagctggag ctgatccctg acccccggaa
     7081 agggtagctc gactttcccc cggcgagggg atatccccgg ggcgtgaggc cccggccttt
     7141 tggccctggg gccttcggtt cgggcggtga acaaaggctc cggcgcgggg acgcccgggg
     7201 cctttttcgt ggtggtggga ggttcagatg gagcacgtgg tggcgtcgtg gggcaaggtg
     7261 aacctgtctc tccgggtgac gggacgacgg gaggacgggt atcatgccct tcactccctt
     7321 tttctccgag tggggccgct ggaagccatg agactccgga tccggtctgg ggctgggagg
     7381 ggagatcttt tgcacctccg gagcgaggtc cccatctcgg gggaaaacct ggtacttcgg
     7441 gccctggccc tggtgagaca gtaccacaag gcattccctt tttttgaagt ggagctgcaa
     7501 aaacagattc ccccggggag tggattgggg gcgggaagcg gcaatgcggc ggcgattctt
     7561 tcctgggccc gtcagcaggg ctttcccgtt cccgacgtct cggcggtggc gtcgctgggg
     7621 gctgatgtgc cctttctcgc ctcctcatgg cccggagggc ggggggaagg cataggggat
     7681 gatctgtccc cattctctct gccagctttg cgagtggccc tggtggtgcc ccggtggagc
     7741 tgcgccactg ggttggcctt tgccgccctg gaccggacta ggggagggga ttggagccct
     7801 ttggaagagg ctcgtcggga gagcgaccgt cttttagaaa accttgcggc gggcaaacgt
     7861 gtggggcttt tgcccaacga ctttggtccg gacctttggg ccgcgcacgg agagtacaag
     7921 gattttttcg acctcgcaga ggagcagggg gccctggggt gggggatcag cggcagcggt
     7981 ggagcggctc tggccctttt tccccgagac aaagcccttc gttggcccga cttttcctgg
     8041 gcccgcatgg ggaccatttt ggaggtgtag gccgtgcgcc ccgcacgtcc cgaacgcctg
     8101 gcccgcctca ccgcccgatt gatgttcgcc ccctctcggc ggatctccct caccgaaaac
     8161 gcgggggagt ttgccatatc caagaccatg gtgggcaatg atttatcgct gctggacgac
     8221 ctcttccagg aggaaggcct ggggagtgtg gtcgtggagc ggggaaggga cgggggggcg
     8281 acctttcgcc cccggctgag ccccgagaga cgccaggaat ggctgaagga agtggcccac
     8341 atcctggaaa cccccgatcg agtgctcccc gggggtctac tctactacag cgatttactc
     8401 ttctctccct tctgggccca aaggctgggc tttgccctgg cctccagctt tgccgactct
     8461 cccatcgacg tggtcatgac cacggaaatg aaaggcattc ctctgggaat gttcacggcc
     8521 catgccttgg gggtgcctct ggcggtctgt cgcttcggga atcgccccag cgatggatcc
     8581 gccgtggtgg ttcactaccc ttcggcctcc ggggacgtgc gaactctgtt catgggagtg
     8641 cgcatgcttc ccaaggcttc ccgagttctt ttggtggacg atgtcatgcg agggggaagc
     8701 accctggcgg gcatgctgca ggtggcgaag gagttcgaag cccaggtctc tggcattggg
     8761 gtctttctcg tggcggagac cccccaggtg aagcgcgtgg gacaccacac ggcgctacta
     8821 cgcttttcca tctcgaccgc cgggatcccc tgcgtggagg tccaagcccc ccctctggcg
     8881 tttgacggag cgggatacgt gcgatacaat cgctccatcg aaagggggcg atgccgtcca
     8941 tggacgtcaa gctgaaccgc aacgattgca tcggctgcgg cgtctgcgtt cagatctgtc
     9001 ccgaggtgtt cgagctggat gaagaggccg gaaaggcccg cgtggcccgt caaggcggga
     9061 cccctgaggc ggtccgcgag gcggtggaga gctgccctgt gggatgcatc tcggtgggat
     9121 aaggcaaata agtcagggtt tccctccctc gttctttggg gcgaggtttt tcgtgggccc
     9181 atagctcaag tggttagagc caccggctca taaccggacg gttcctggtt cgatcccagg
     9241 tgggcccacc acggcgaatg cgagccacct tcgggtggct ttttctgtgc gaccccgtcg
     9301 gaccgagtat ggggggtccg aaggaaggag gcccctccta tgaaaaccgt aaaggacttg
     9361 atgacgacct tggaaatcat cgccccctcc cgaggcatgg aggaatggga caacggagga
     9421 ttgctgctag ggagccccca ttggcccttg ggtcgcgtgg gagttgccct ggacccttcc
     9481 gaagaaatag tgagtgcggc agtgtgccgg aagtgttctg cggtggtcac ccatcacccc
     9541 atgaccttga cgggatggaa agccctgtcc ctggaaaccc ccgaggggcg cctggtctcc
     9601 cgagccctgg gggaatcggt ggccctggtg gcggcccaca ccaactggga tcgggcggag
     9661 gaaggggtga atgtagcctt ggggaaggcc cttcccctgt ctggcatgca tcccctatgc
     9721 tcctcccggg aggaagggct ctggggggac ggggggtggg gagatctggt tactcctctt
     9781 cccgtccggg aaatggccca gaacgtgggg cgcctgtggg ggctgagttg ggggcatctt
     9841 ttttccccgg atccctctct ggtcgtgtct cgactggctt gggtcggtgg atcggggggg
     9901 agtttctggc gcagggcccg tgaaaagggg gccacccttt tctgcacggc ggacatgaag
     9961 tatcatgagc gacgggacgc catggcggcg ggtttggccc tcctggtggt ggaccatgga
    10021 gagatggaaa gcttttccct gggatccttg gcccggttga tccaaagcca tctgggccag
    10081 gaggtggaga tattggcgga gcccccttct tacggatcca ttcttttgtt ttaacgcaat
    10141 ggaggaggcc ccggggcaag aggggggcga gcgcctcttc tcggggggat tggagaatac
    10201 gtaaagggcc gaaaggggcc gcagggccct tgacgatggc ggccatgact cgggaacgga
    10261 gcgtgaaaag tatcatgcga gaaagagtgt tcagcggcat gcgacccacc gggcggcttc
    10321 acctgggaca cctggcgggg gccctgacca attgggtgcg ccttcaggat gactacgagt
    10381 gtttctactg catcgtggat tggcacgccc tcatgtccga ctacgcggac tcgcgccagc
    10441 ttgcggctaa cggccaggag atcctattgg attggctcgc agtggggctc gatcccgcca
    10501 agagcgccat tttcgtccaa tcccacgtgg cggagcacgc cgagctccat ctggccctat
    10561 ccatgatcac ccccctgggg tggttggaga ggtgccccac gtacaaagat cagatcataa
    10621 acatgaaaaa caaagatcta agcaactacg cctttctggg gtacccggtg ctgatggcgg
    10681 cggatatcat tctctacaag gcccacaaag tgcccgtggg cgaggaccag tccgctcacc
    10741 tggaactcac ccgggaagtg gtgagacgct tcaaccactt ttacggcgat gtctttcccg
    10801 agcccgacat cctccttact cccaccccca aggtcccggg caccgatggc cgtaagatga
    10861 gcaagtccta cgggaactcc ctggccatcg ccgatagcct ggaggaaatc tgggagcgga
    10921 tccgcaccat gctcaccgac ccggcccgca tccgccggac cgatccgggg gatccggaaa
    10981 agtgccccgt ctgggatctc cataaggtgt tcaatgcgga cgcggccgag agagaagaag
    11041 tggcttcggg gtgccggagt gccgccatgg ggtgcatcga gtgcaaaaaa aagctccacg
    11101 gccacatttg tacccttctg gagcccatcc aggagcggag gcggtattat gaaacccatg
    11161 gcagcgcttt ggaggaggtc ctccaccagg gagcccagaa ggcccgcgtg gtggcggagg
    11221 ccaccatgca ccacgtccga agcgccatgg gcctgcagcc tcctcgatag aaggcccctt
    11281 tcgccggggg acgaggagag cgatcccgag gtcctttgtc gtgcctttgt cctttgaagt
    11341 caccctggag gggttttccg gccccctgga ccttctttgt gcgctggtgg aatctcgagc
    11401 cattgcggcg tctcgagtgc gggtatccga cgtggtgcgc ctttacggcg cctacctcgc
    11461 tcggggggac gtgatgacca t
//
LOCUS       DAOOER010000055        11467 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_6181_length_11467_cov_8.254469, whole genome shotgun sequence.
ACCESSION   DAOOER010000055 DAOOER010000000
VERSION     DAOOER010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 11467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11467
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6181_length_11467_cov_8.254469"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PL162_07375"
     CDS             complement(<1..519)
                     /locus_tag="PL162_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91872.1"
                     /translation="MQRSWGMSVLMLVCVFAFAALGLAPASWWQEQFSFGQGEPVEFV
                     VPEGVSEKQLADLVVAQKITGQAEELADLLTDMGVVKTLRSGIYAARPGTPWEVAMQL
                     QGQSPRAELFVLEEGIMFRDLSAKLPGGGTEGLRLLASDDLFPEALRPLLPGDPESRA
                     ALLLPGAYVLSPD"
     gene            complement(763..1512)
                     /gene="rnc"
                     /locus_tag="PL162_07380"
     CDS             complement(763..1512)
                     /gene="rnc"
                     /locus_tag="PL162_07380"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HQF91873.1"
                     /translation="MAMDQAWWDKSLTAFQREIGYRFQNERLIHEALTHASFAHEAGI
                     PFWNERLEFLGDAVLELCVSEMLFVEYAHWDEGLLTQERARLVCEGALARLATQLNIP
                     PLLRMGRGLERQGGRENASICADAVESVLGAIFLDGGFDAARQVVRRLLLIFRHHARD
                     EMIDPKSKLQQMFQEKGWGRPEYDLVSTEGPSHDMVFRVTVRHRQTILGSGTGATRKE
                     AEVAASRAALEALLPRRKQGPYSSVKHDAEF"
     gene            complement(1521..2759)
                     /gene="fabF"
                     /locus_tag="PL162_07385"
     CDS             complement(1521..2759)
                     /gene="fabF"
                     /locus_tag="PL162_07385"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HQF91874.1"
                     /translation="MRRVVITGIGVVSPIGHGREVYWNALAAGKNGIGPVTLFDTTDF
                     PVRIAAEVRDFDAGLYMDRKEIRRSDRVIHFSVAAAAMAVEDAKLDEGSLDRTRYGVY
                     IGSGEGGISTTNENYRILYEKGPTRISPFFIPMMISNMPAAYVAMVHGAKGPNLSVVT
                     ACATATNTIGEAYYAILRDDADVILTGGAEAAIVPIALAGFAAAKTLSTRNDEPEKAS
                     RPFDVDRDGFVMGEGAGVLVLEELEHAKNRGARIWGEIVGYGATCDAYHITAPSPDGD
                     GQIRAMAMAVRKAGWRKEDVELINAHGTSTPLNDKTESLAIRTLFGDYTDKLLVNSTK
                     SLVGHGLGAAGALETIAAIQSLVEGIVHPTLNLDSKDPECDVPVVGKEAIHVPIRHVL
                     VNNFGFGGHNGVLALTRFEE"
     gene            complement(2899..3156)
                     /gene="acpP"
                     /locus_tag="PL162_07390"
     CDS             complement(2899..3156)
                     /gene="acpP"
                     /locus_tag="PL162_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQF91875.1"
                     /translation="MGSNVGEVTARLKEIVMDRLDVEENQISETASFVEDLGADSLDI
                     VELIMGIEEEFDIEIPDEDAEKLTNVGEALAYIQKKLGIEA"
     gene            complement(3186..3932)
                     /gene="fabG"
                     /locus_tag="PL162_07395"
     CDS             complement(3186..3932)
                     /gene="fabG"
                     /locus_tag="PL162_07395"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HQF91876.1"
                     /translation="MASMKKVALITGAGRGIGRAIAREMAARGFAVAINYHSSEEGAR
                     ALVEELREAGHEAHAFQGNVADPASVKDLFAQVGSTMGAPAVLVNNAGIVRDTLVLRM
                     KDQDWAEVVQTNLTSAFLCSREAVREMSRQRWGRVVMISSVIGLMGNTGQANYAAAKA
                     GLFGLAKSLAREFGSRQITVNAVAPGFIETDMSGRIKEEFREAYLKNIPLARSGTPED
                     VARVVAFLVSDDASYVTGQVLAVDGGLTMC"
     gene            complement(3934..4881)
                     /gene="fabD"
                     /locus_tag="PL162_07400"
     CDS             complement(3934..4881)
                     /gene="fabD"
                     /locus_tag="PL162_07400"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HQF91877.1"
                     /translation="MSYALLFPGQGAQEVGMGQDLAKAYPSARETFEAADEALGFSLS
                     QIVFHGPEETLQETTYTQPAILVTGVALARVLTGDLGVSLTPSFAAGHSLGEYTAHVM
                     AGTLSLEDAVRLVHLRGRYMQEAVPLGEGSMAALLGLDPAAVPEVCQQAAEGQVCEAA
                     NFNAPGQVVISGHTAAVERAGGLAKKMGAKKVIPLNVSAPFHCSLMRPVAQRLAEAFE
                     GVMWNDPSFPVVSNVSALPMDHQEDVRKALCAQTYSPVRWVESVETMVRAGTNTFLEF
                     GPGNILSALVKKINKNAKTLSLRSGEELREAVDFLRGGQ"
     gene            complement(4941..5894)
                     /gene="fabK"
                     /locus_tag="PL162_07405"
     CDS             complement(4941..5894)
                     /gene="fabK"
                     /locus_tag="PL162_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HQF91878.1"
                     /translation="MRFPTRITEMLHIPFPIFQGGMAWVADARLAAAVSNGGGLGIIA
                     AANMPPDLLDQELHTVRTLTDRPFGLNVMLLSPTAADATELAARHRVPVVTTGAGAPG
                     KVIDRLKPLGIRVIPVIASVSHARRVAKQGADAVVAEGTEAGGHIGESTTLALVPQVV
                     DAIPIPVIAAGGIADGRGVAAAFALGAEGVQLGTRFVCAQECTVHEAYKRAIIDAGDR
                     ETCVTGRPTGHPVRCLKNKLTRAFEELEKSGAPLEEYERLGVGRLRSGVREGDVDWGS
                     LMAGQVAGLVRKIQPASEIIQEIFNEAGDVLERLRVRFPGP"
     gene            complement(5905..6903)
                     /locus_tag="PL162_07410"
     CDS             complement(5905..6903)
                     /locus_tag="PL162_07410"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HQF91879.1"
                     /translation="MTLFTGRAVSLWSTGVYVPQRVLTNADLEKMVDTSDAWIQERSG
                     IRQRHIASPDEQTSDLAAQAGRGALAKAGVSPEELDMIIVATNSPDTIFPGVAPKVQG
                     LLGAPRAGAFDVQAGCTSCVYALANAVAGVSSGLWNRVLVVGAEALSRLIDWQDRNTC
                     VLFGDGAGACVIGPQRAGDGRFLASALRSDGTKSDLITLPAGGTAMPASEESVAGKQH
                     FVHMKGNEVFRFVNRELPPFLGEFCQVTGVPPKEVDWWIFHQANLRIIEGVLRRLDIS
                     GDRTVVNLDRYGNTSSASVFIALHEAIENGQIRPGHKVILNSFGAGMTYGALLLEM"
     gene            complement(6900..7904)
                     /gene="plsX"
                     /locus_tag="PL162_07415"
     CDS             complement(6900..7904)
                     /gene="plsX"
                     /locus_tag="PL162_07415"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HQF91880.1"
                     /translation="MIVVDAMGGDHAPAEICRGAVQACRQNPNLEIALVGKGELLGPL
                     LDEAASGERKRLHIVPAEEVIAMDETPAVAIRKKRKASLCVAMHMVRSGEAQGCVSAG
                     NTGAIVAGGVLIVGRIPGIDRPGLGVPLPTQKRMSLLLDVGGTIRCKPLNLYQFALMG
                     EVYMRSIWGVKEPEIALLSNGEEEIKGDDVVAEARELIRQGGHRFVGYVEGKDIPVGL
                     ADVVVTDGFTGNILLKFAEGVGRAAYDIVREEMGHRLLPRVGVLFLWPMLRSLWHRFD
                     YERYGGTPLLGVSGAVIKAHGRSKAPAIENAIKVAQNFVDQGGVHRIRDEIVRRGDQA
                     "
     gene            complement(7930..8538)
                     /gene="fapR"
                     /locus_tag="PL162_07420"
     CDS             complement(7930..8538)
                     /gene="fapR"
                     /locus_tag="PL162_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045717 - negative regulation of fatty acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HQF91881.1"
                     /translation="MSRSRERKLRHDRLLSLLEENPLHTDEDLAHALKASISTVRLDR
                     ALLGVPELRERMRHMAEKATSKLRSLAQDDVVGDLLELEPNMWALSVLQTGKEMAFRH
                     SSLVWDHHIYAQASSLAMAVIGADMVVTGAARARYRAPVRVGDKLIARAKVGISKGNK
                     YVVSVRTKVEGREIFTGRFIVVVLEDGEYQALEEQGLWTQEE"
     gene            complement(8877..9068)
                     /gene="rpmF"
                     /locus_tag="PL162_07425"
     CDS             complement(8877..9068)
                     /gene="rpmF"
                     /locus_tag="PL162_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HQF91882.1"
                     /translation="MAVPKRRVSHRKTAHRKAAWLRALSAPALTSCPHCGEKIQAYRA
                     CPACGYYRGRKAVKSAEEA"
     gene            complement(9092..9463)
                     /locus_tag="PL162_07430"
     CDS             complement(9092..9463)
                     /locus_tag="PL162_07430"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HQF91883.1"
                     /translation="MALRVEGTGEVSCARCLVPTPLAIRGDFVYFFLLRRKEEEFPGE
                     GEEAEEHLVELERWPEKLSLAAYAWETLVAEIPSRVLCAEGCLGLCSRCGVNRNQESC
                     SCEPAGGDLRFLKLKERPMDR"
     gene            complement(9688..10881)
                     /locus_tag="PL162_07435"
     CDS             complement(9688..10881)
                     /locus_tag="PL162_07435"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008520725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HQF91884.1"
                     /translation="MKVLVLNCGSSSLKYQLFDMSTEEVLSKGLVERIGIAGSRIKHV
                     KSGMEPVVTDVDIPNHKVGIKFVLEALLSSEHGSLNSLDELRAVGHRVVHGGEKFARS
                     AIVTDEVLKAIEDCTPFAPLHNPANLMGIRAMVEVLPKVPQVAVFDTAFHQTMPETAY
                     TYGIPYHYYEDLRIRRYGFHGTSHFYVAHRAAEMLNRPIESMNIVTCHLGNGSSLSAV
                     KKGKSVDTSLGFSTVTGVLMGTRCGDVDPVALIHLMQEDGMDAQSLSHMLHKESGIAG
                     ISGLSSDLRDVEDAAERGEHRSALALDMLCYGIRKYIGAYAAAMGGVDAIVFTAGIGE
                     NSTTVRAKSCEGLEFLGARIDLDKNNIRGKEAVVSTDDSRVPILVIPTNEELVIARDT
                     VTLTA"
     gene            10946..>11467
                     /locus_tag="PL162_07440"
     CDS             10946..>11467
                     /locus_tag="PL162_07440"
                     /inference="COORDINATES: protein motif:HMM:NF017452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HQF91885.1"
                     /translation="MSEAVVGIIAEYNPLHNGHFHHLARAREESGARGVVVVLSSYFV
                     QRGEPAMVDKWARTAMALACGADVVLELPTVFSCHNAGVFANAAVDLLAALGGVTHVA
                     FGMEEPQGDLETLGAILMEEPEAFKSALQRFLQEGFSFVESRSRALETLVPGSASLLR
                     GSNNTLALAYCCRI"
BASE COUNT         2199 a         3619 c         3281 g         2368 t
ORIGIN      
        1 gtcgggagac aggacataag cgccgggaag gagaagggcc gcccgacttt ccgggtcgcc
       61 gggcagcaag ggcctcaagg cctcggggaa gaggtcgtcg ctggccaaaa gcctcaggcc
      121 ttcggtcccg ccgccgggga gcttggccga cagatctcga aacataatcc cctcttccag
      181 gacaaagagc tccgcccgag gactctggcc ctggagctgc atggccacct cccagggcgt
      241 cccggggcgc gccgcataaa tccccgaccg gagggttttg actactccca tgtccgtcag
      301 gagatcggcc aactcttcgg cttgaccggt gatcttctgc gctacgacaa gatccgcgag
      361 ctgtttttcg gagacacctt cagggacgac gaattccacg ggctctccct gaccaaagga
      421 aaactgctcc tgccaccacg aggcaggggc gaggcccaag gccgcaaacg cgaatacgca
      481 caccaacatc aggacgctca taccccaaga ccgctgcatc aatcccgacc tccccgattt
      541 agatacacaa aaagacgcag gaacaatttc ccacatcttg ccgtaagcca ttatagcgcg
      601 tcttctctcg ggagaacacc tcaaaaaaac ggtccttgtc cgccgctgtc cgataaggct
      661 taccggaaaa agcctccgaa ccgccaaaag gggcagttta tcgccttccc tccggcctag
      721 aacatcaatc ccgatgatag gacgtatagg gatcttttta aattaaaatt cggcgtcatg
      781 ttttaccgag gagtacggtc cctgttttcg gcgaggaagc aaggcctcca gggcggcccg
      841 agatgcggcc acctcggcct ctttccgcgt ggcccctgtg ccggatccga ggatcgtctg
      901 gcgatggcgc acggtcaccc gaaacaccat gtcgtgcgac ggcccctccg tgctgaccaa
      961 gtcgtactcc ggcctccccc atcctttttc ttggaacatc tgctgaagct tgcttttggg
     1021 gtctatcatt tcgtctcggg cgtggtgacg aaaaatcagc agaagacgcc gcaccacttg
     1081 ccgtgcggcg tcaaacccac catcaagaaa gatcgctccc aaaacggact ccaccgcgtc
     1141 ggcgcagatg gaggcgtttt ctcgtccccc ttggcgctcc aggccgcgcc ccatgcgcag
     1201 tagcggaggg atgttcagct gcgtggctag ccgcgccagg gctccttcgc acaccagtcg
     1261 agcgcgctcc tgagtcagca gcccttcgtc ccaatgggca tattccacga agagcatttc
     1321 ggagacgcag agctccagca ccgcgtcgcc caggaactcc agacgctcgt tccaaaaggg
     1381 gatccccgcc tcatgggcaa acgaagcatg ggtaagcgcc tcgtgtatca aacgctcgtt
     1441 ttggaaacgg tacccgatct ctcgctgaaa ggccgtgaga cttttgtccc accaggcctg
     1501 gtccatggcc atgagagaag ctattcctca aaacgggtca gtgccagaac gccgttatgt
     1561 cctccaaagc cgaaattatt taccaaaacg tgccggatgg gcacatggat cgcctctttc
     1621 cccaccacgg ggacgtcgca ttcgggatct tttgagtcga gattgagtgt ggggtggaca
     1681 atcccctcta cgagggattg gatcgccgca atggtctcca gggcgcccgc agcgcccagg
     1741 ccgtgtccca ccaaggattt ggtggagttg accaggagct tgtctgtgta atccccgaag
     1801 agggtcctga tggccaggga ttcggtcttg tcgttcagcg gcgtggaggt cccatgggca
     1861 tttatgagct ccacgtcttc tttgcgccac cccgcctttc gcaccgccat ggccatggcg
     1921 cggatttgac catctccgtc ggggctcgga gccgtgatat ggtacgcatc gcaggtggcc
     1981 ccataaccca cgatctcgcc ccagattcgg gcgcctcgat ttttcgcgtg ttctagctct
     2041 tccagaacga ggactccggc cccctctccc atgacgaacc cgtcccgatc cacgtcgaag
     2101 ggacgggacg ccttttcggg ctcatcgttg cgggtggaga gggtcttggc ggcggcaaac
     2161 cccgccaggg ctatgggaac gatggccgct tcggctcccc cggtgaggat cacgtcggcg
     2221 tcatctcgta aaatggcgta gtatgcctct ccgatggtat tggtggccgt ggcgcaggcg
     2281 gtcaccacgg aaaggttagg tcccttggct ccatgcacca tggccacgta ggcggcgggc
     2341 atattgctga tcatcatggg gatgaaaaag ggactgattc tagtggggcc tttctcgtag
     2401 aggatgcggt agttctcgtt ggtggtgctg atacccccct caccgctacc aatgtagacg
     2461 ccgtagcggg ttcgatccag ggagccctcg tccaatttgg catcttccac cgccatggcg
     2521 gcggcggcca cggaaaaatg gatgacccgg tcggagcgac gaatctcttt gcggtccatg
     2581 taaaggcccg cgtcaaagtc ccgtacctct gcggcaatgc gcacaggaaa atccgtggtg
     2641 tcaaaaagcg tgaccgggcc gatgccgttt tttcccgccg ccagggcgtt ccaataaact
     2701 tcccgaccgt gcccgatggg gctcaccacg ccgatcccgg taataacgac tcttctcaac
     2761 gccgggttcc tccttaatat ctcgtttatg aaaaaccgac gcacctacca ttgcccactg
     2821 aaacatctcg aaggggcgga gacgggcggt ttcccgatta tagtcaaaga gaggggggca
     2881 tggcccccct cggtggagtt atgcttcaat gccaagtttc ttctgaatgt acgccagcgc
     2941 ttcgccgacg ttggtgagtt tctccgcatc ttcgtcggga atttcaatgt caaattcctc
     3001 ttcaatcccc atgatcaatt ccacgatgtc cagagaatcg gcccccagat cctctacgaa
     3061 cgacgccgtt tcggaaatct ggttttcttc cacgtccagg cgatccatca cgatctcctt
     3121 gagacgggcg gttacttctc ccacattact ccccatactg ttcacctcct tttcgatgtt
     3181 cttctttagc acatggtcag gccgccatcg acggcgagca cctgccctgt gacgtaggat
     3241 gcgtcatcgg agacgaggaa ggccaccact cgggccacgt cctccggggt ccccgatcgg
     3301 gcgaggggaa tatttttcag gtacgcctcc cgaaactctt cttttatgcg accgctcata
     3361 tcggtttcaa taaaaccggg agccaccgca ttgacggtga tctggcggct tccgaactct
     3421 cgggcaaggc tcttggcgag tccgaaaagg cccgctttgg cggcagcgta attggcctgc
     3481 cccgtgtttc ccatgaggcc gatgacggag ctgatcatga caactcttcc ccaacgctgc
     3541 cgggacatct cccgcacggc ctctcgagag cagagaaagg ccgacgtcag gttggtctgc
     3601 acaacctcag cccagtcctg gtccttcatg cgcaacacca aggtgtccct tacgatgcct
     3661 gcgttgttga cgagcaccgc cggggctccc atggtgctcc ccacctgggc aaagaggtcc
     3721 ttgacggaag cggggtcggc cacgttcccc tgaaaggcgt gggcctcatg ccccgcttct
     3781 ctcaattcct ccaccagggc gcgggcccct tcctccgagc tgtgatagtt tatggctaca
     3841 gcaaaccctc gcgccgccat ctctcgggcg atggcgcggc caatgccccg tcccgccccc
     3901 gtgatcaggg ctactttctt catagacgcc attttattga cctcctcgaa gaaaatcaac
     3961 tgcctcgcgc agttcctctc cggagcgaag ggaaagggtt ttggcgtttt tgtttatttt
     4021 tttaacaagg gctgagagga tgttccctgg cccaaactcg agaaaagtgt tcgtccccgc
     4081 tcgtaccatg gtttccaccg actctaccca tcgcacgggg ctgtaggttt gggcgcatag
     4141 ggccttccgg acatcctcct ggtgatccat gggaagagcg gagacgttgg ataccacagg
     4201 gaaggaggga tcgttccaca ttaccccctc aaaggcctcg gccaaccgct gagccaccgg
     4261 tcgcatcaga gaacaatgaa agggagcgct gacgttcagg gggataacct ttttggcccc
     4321 catctttttt gccaggcccc ccgctcgctc caccgccgcc gtatgtcccg agatgaccac
     4381 ctgtcccggg gcgttaaagt tggccgcttc gcacacctgc ccttctgccg cctgttgaca
     4441 cacttcgggc accgcagcgg gatccaaccc gagcagggcg gccatgctac cttcccctag
     4501 gggtacggcc tcctgcatgt acctccctcg gaggtggacc aatcgaacgg cgtcctccag
     4561 ggacaaggtt ccggccatca cgtgggccgt atactcccct aaactgtgcc cggcggcgaa
     4621 ggagggcgtc aaagacaccc ccaggtcccc ggtcagcacc cgggccaggg ccactccggt
     4681 gaccaggatg gcgggctggg tgtaggtcgt ctcctgaagg gtctcttcgg gaccatgaaa
     4741 gacgatctgg ctgagggaaa aacccagagc ctcatcggcc gcctcaaagg tctccctcgc
     4801 cgagggatag gccttggcaa gatcttgccc catgcccact tcttgggccc cctggccggg
     4861 aaaaagcagt gcgtatgaca tgagcgaatc cctctctttt ccctgaaaaa tgtgtcggcc
     4921 tgccgcccag gtctttcggc ctagggtccg gggaagcgaa cgcggagtct ctctaggacg
     4981 tccccggcct cattaaatat ttcctggatg atctccgatg cgggctgaat ttttcgcacc
     5041 aatcccgcca cctgccccgc catgagggag ccccaatcca cgtctccctc tcggacgcca
     5101 ctccggagac gccccacccc cagcctctcg tattcttcca ggggagcgcc agatttttcg
     5161 agctcctcga aggcgcgggt gagcttattt ttcaggcacc gcaccggatg tcccgtgggg
     5221 cgccccgtca cgcaggtttc tcggtccccc gcatcgatga tggccctttt gtaggcctcg
     5281 tgcaccgtgc actcttgggc gcagacaaag cgggtgccca gttgcacccc ttcggccccc
     5341 agagcgaagg ccgcagccac tcctcggcca tcggcgatgc ctccggcggc gatgacggga
     5401 atgggtatag catcgaccac ctggggcaca agggcaaggg tggtggattc tccgatgtgg
     5461 cctccggcct cggtaccttc ggccaccacc gcgtccgccc cctgtttggc cacacgtcgg
     5521 gcgtggctca ccgatgcgat gaccggaata acccggatcc ccaggggttt taggcggtcg
     5581 atgaccttgc cgggggctcc cgctccggtg gtgaccaccg ggacgcgatg gcgggcggcc
     5641 agctccgtcg cgtcggcggc ggtgggagaa aggagcataa cgttcaaccc gaaagggcgg
     5701 tccgtcaagg ttcgtaccgt gtgtagctct tggtcaagaa ggtccggagg catattggcc
     5761 gccgcgatga tgcccaaccc gcctccattg ctcaccgccg ccgccaggcg ggcgtccgcc
     5821 acccaggcca tgcctccctg gaaaatcgga aaaggaatgt ggagcatctc ggtgatacga
     5881 gtgggaaagc gcaccaaaac cacctcacat ttccaaaagc agggctccgt aggtcatgcc
     5941 cgccccgaaa ctgttcagga tcaccttgtg gcccggacgg atttggccgt tttcgatggc
     6001 ttcgtggagc gcgatgaaga ccgaggcgga ggaagtatta ccgtagcgat ccaagttgac
     6061 cacggttcta tccccggaga tgtccaggcg ccgcaggacg ccttcgatga tgcgaaggtt
     6121 tgcctggtgg aagatccacc aatccacttc tttcggaggc acgccggtga cttgacaaaa
     6181 ttccccgagg aaaggaggaa gctctcggtt gacgaaacga aaaacctcgt ttcctttcat
     6241 gtgtacgaag tgctgttttc ccgcgacgct ttcttcggag gcgggcatag cggtccctcc
     6301 cgccgggagg gtgatcagat cgctcttagt tccgtcggag cggagagcgg aggccaaaaa
     6361 acgcccatcg ccagctcgtt gggggcctat gacgcacgcc ccggcgccgt cgccgaaaag
     6421 aacgcaggtg ttgcgatcct gccagtcgat gagacgggag agggcctcgg cgccgacaac
     6481 cagcacccgg ttccaaagcc ccgaggagac ccctgccacc gcgttcgcca gggcgtagac
     6541 gcagctggtg caccccgcct ggacgtcgaa ggccccagcc cggggagccc ccaaaaggcc
     6601 ctgaaccttg ggggccactc cgggaaaaat cgtgtccggg gagttggtag ccactatgat
     6661 catatccaat tcttcgggcg aaacccctgc tttagccagg gcacctctcc cggcctgggc
     6721 cgctaggtcg ctggtttgtt cgtcgggaga ggcgatgtgg cgctggcgga tgcccgatcg
     6781 ttcctgaatc catgcgtcgc tggtgtctac cattttctcc agatcggcat tggtaagcac
     6841 ccgctgcggc acgtacaccc ccgtgctcca gaggctcacc gctcttccgg taaatagggt
     6901 catgcttggt ctccccttcg gacaatttcg tcgcggatcc gatggactcc gccctgatcg
     6961 acaaagtttt gggccacctt gatggcattc tctatggcgg gagccttgga ccgtccgtgg
     7021 gccttgatga cggcaccgct cactcccaaa aggggtgtgc cgccgtagcg ctcgtagtcg
     7081 aagcgatgcc aaaggctacg tagcatgggc cacaggaaca gaacccccac ccgagggagg
     7141 aggcggtgcc ccatttcctc ccggacgatg tcgtaggcgg cccggcccac gccttcggcg
     7201 aactttaaca ggatgtttcc cgtgaagcca tcggtaacca ccacatcggc cagtcctacg
     7261 gggatgtctt tcccttccac gtagcccacg aatcggtgtc ccccttggcg gatcaactcc
     7321 cgggcctccg ccaccacatc gtcgcctttt atttcttcct cgccgttgga aagcagggca
     7381 atctctggct cttttactcc ccagatgctc cgcatgtaga cctcgcccat gagggcaaac
     7441 tggtaaaggt tcaggggttt gcaccgaatg gtgcccccca catccagcag gagggacatg
     7501 cgcttctggg tgggtagagg cacccctaat ccggggcgat caatgcccgg gatgcgtccc
     7561 acgatgagca ctcctccggc cacgatggcc cccgtgtttc ccgccgaaac gcacccctgg
     7621 gcctcgccgg atcgcaccat gtgcatggcc acgcagaggc ttgcctttct cttttttcgt
     7681 atggccaccg ccggagtttc gtccatggcg atgacctcct cggcgggaac gatgtgaagc
     7741 cgctttcgct cccccgatgc cgcctcgtct agaaggggac ccaggagctc ccccttcccc
     7801 accagagcta tctccaaatt ggggttttgg cgacatgcct gcaccgctcc ccgacaaatt
     7861 tccgccgggg cgtggtcgcc gcccatggca tcgacgacga tcatgccacc atcgctctcc
     7921 tttgactttt cattcttctt gggtccataa gccctgttcc tccaaggcct gatattctcc
     7981 gtcttccagc accacgacga tgaaacgacc ggtaaaaatt tcccgccctt ccaccttggt
     8041 ccggacgctg accacgtact tatttccttt ggaaattccc accttggctc gggcgatgag
     8101 cttatccccc acccggaccg gggcgcgata gcgtgcccga gccgctccgg taaccaccat
     8161 gtcggccccg atgacggcca tggccaggga gctggcttgg gcgtagatgt ggtgatccca
     8221 gaccagggaa gaatggcgaa aggccatttc cttcccagtc tgcaacaccg acagggccca
     8281 catgttgggt tcaagctcca gaagatctcc caccacgtcg tcttgagcca gggatcgaag
     8341 cttgctggtg gccttttctg ccatgtgccg catacgctcg cgcaactcgg gcacccccag
     8401 gagtgcccgg tccagacgca ccgtgctgat gctcgccttt agggcgtgag ccaagtcctc
     8461 gtccgtgtga agggggtttt cctccagcag ggagagaaga cgatcgtggc gcaattttcg
     8521 ttctcgggat cgactcagga ggatcccccc cttctattta tcgcctggtg ataacaactt
     8581 gtagtggcag gagtatagaa gatgaggtgt catttcgtca agggcttctc tctggctgag
     8641 tttcaccgcc tcgtttctat ggcggtattg ccggtgattt atggatttcc ttggggtagt
     8701 agaggggcaa aggcggtgga ttagggcggg caggcgctac ggcaatggga tcaaaggggg
     8761 cagtctacgg agggccgcag ggaggacagt tttagagttc cgtagtcttt gtgaacgacg
     8821 gcgccgggac tggtttcgcg tcccggcgcc acacagtaga ccctatcgca ggatgactag
     8881 gcttcttccg cgctttttac cgccttgcgg cctcgataat atccgcacgc ggggcatgcg
     8941 cggtatgcct ggatcttctc gccgcaatgc gggcagctgg tcagtgcggg agcgctcaac
     9001 gcccgaagcc aagcggcctt gcgatgagca gtcttacggt gtgaaactct tctctttggc
     9061 acagccatgt cttttccccc tttttttcct actaccgatc catgggccgt tctttcagct
     9121 tcagaaagcg tagatcaccg cctgcaggct cgcaagagca gctttcctgg tttcggttca
     9181 cgccacagcg cgaacacagg cccaaacatc cttcggcgca caaaacccgg gaaggaattt
     9241 ccgccaccag cgtttcccag gcgtatgccg cgagcgacag cttttccggc caccgctcca
     9301 gctccaccag gtgttcttcg gcttcttcac cttcgccggg aaactcttcc tctttcctcc
     9361 gcagaaggaa aaaatacacg aaatcgcccc gaatggcaag gggggtaggg acaagacatc
     9421 gggcacacga cacctctccg gtcccctcca ctcggagggc cacctcgaag gagtccttct
     9481 cccaccgaca tcgagcgtga acgcgaaccc cctgggggaa acaaaatttt tgtccccaat
     9541 gatccacccc aaagggaccc tctaggaccc actcccgttc ttctgtctct tctcggccga
     9601 tcctcatagg gtagacggct aagtcccacg tggggggagc tcccctccag ggggaggtcg
     9661 gagtaatccc ttccgacaag gagaccatta cgctgtgagg gtgacggtat cccgggcgat
     9721 gaccagttct tcgttggtcg gtatgaccag aatgggcacc cgggaatcgt cggtgctgac
     9781 cactgcttct ttgcctcgga tattattttt atccagatcg atgcgggccc ccaggaattc
     9841 caatccttcg caggatttag cgcgcacagt ggtgctgttt tctccgatgc ccgccgtgaa
     9901 gacgatggca tccaccccac ccatggcggc ggcataggcg ccgatgtact tgcgaatgcc
     9961 gtagcaaagc atatccaacg ccagggcgga gcggtgctcg cccctttcag cggcgtcttc
    10021 cacatcgcgg agatcgctgc taaggccgga aatgcctgcg atgccgcttt ctttgtgcag
    10081 catgtggctc agggactggg catccatgcc atcttcctgc ataaggtgga tcaaggccac
    10141 ggggtccaca tcgccgcacc gggtgcccat cagcacgccg gtcaccgtgc tgaaacccag
    10201 ggaggtatcc acagatttcc ccttctttac ggcgctcagg gaactgccgt tgcccagatg
    10261 gcaggtgacg atgttcatgg attcgatggg tcgattcagc atctcggcgg ctcggtgcgc
    10321 cacgtagaag tgactcgtgc catgaaaacc gtagcgccgg atgcggaggt cctcatagta
    10381 gtgatagggg atgccgtagg tgtaagccgt ctcgggcatg gtctggtgga acgccgtgtc
    10441 gaaaacggcc acctggggga ccttggggag gacttccacc atggcccgaa tacccatgag
    10501 gttggccggg ttatggagcg gagcgaaggg agtgcagtct tcgatggcct tgaggacctc
    10561 gtcggtgacg atggccgatc gggcgaactt ctcgcccccg tggacaaccc ggtgtcccac
    10621 cgccctcaac tcgtccaggc tgttcaagga tccgtgctcc gaggagagaa gggcttcgag
    10681 gacaaacttg atgcccacct tatggttggg gatatccaca tcggtgacca cgggttccat
    10741 gccgcttttg acgtgcttga tccgggatcc ggcgatgccg atgcgctcca cgagcccctt
    10801 gctgagcacc tcttccgtgc tcatgtcgaa aagctggtac ttcaacgaag aactcccaca
    10861 attcaaaacc agaactttca ccaatgccgc ctccttgacg ttgggcttgc catccgcata
    10921 aataatggat acggggggga tggtcatgtc tgaagccgtc gtgggtatca tagcggaata
    10981 caatccactg cacaacgggc actttcatca tcttgcgcgg gcccgggagg agagtggggc
    11041 tcgaggcgtg gtggtggtgc tttccagcta ctttgtccag cgcggagagc ccgccatggt
    11101 ggacaaatgg gcgcgcaccg ccatggccct ggcctgtggc gccgacgtgg tcctggagct
    11161 tcccaccgtt ttttcctgcc ataacgccgg agtcttcgcc aacgccgccg tggaccttct
    11221 cgcggcgttg ggaggagtca cccacgtggc cttcggcatg gaagaacctc agggagatct
    11281 ggagaccctt ggggccatcc tcatggaaga gcccgaagcc tttaaatccg cccttcagcg
    11341 ctttctccag gagggttttt ccttcgtgga atcccggtcc cgagcactgg aaaccttggt
    11401 gccaggtagc gcctccctcc ttcgggggtc aaacaacacc ctggcgctgg cctactgctg
    11461 tcgcatt
//
LOCUS       DAOOER010000056        10832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_6578_length_10832_cov_7.924283, whole genome shotgun sequence.
ACCESSION   DAOOER010000056 DAOOER010000000
VERSION     DAOOER010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 10832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10832
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6578_length_10832_cov_7.924283"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /gene="cas5c"
                     /locus_tag="PL162_07445"
     CDS             complement(<1..356)
                     /gene="cas5c"
                     /locus_tag="PL162_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HQF91886.1"
                     /translation="MSKAFFDKSFCLEVWGDYACFTRPEMKVERVSYDVITPSAARGV
                     FEAVFWKPAVRWRVQKIEVLNPIKWISVRRNEVGLVMSDRSDGFFVEEQRQQRAGLFL
                     RDVRYRIHAAMEFIPPR"
     gene            complement(386..>2125)
                     /gene="cas3"
                     /locus_tag="PL162_07450"
     CDS             complement(386..>2125)
                     /gene="cas3"
                     /locus_tag="PL162_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HQF91887.1"
                     /translation="FLDTEMFMEPEKAESRGGHASLAALKDRFDAYMERKVCEAEDSL
                     INRKRREILEQCRRKAAEKPGFYTLTVPTGGGKTLSSMAFALEHALAHNKRRIIMAIP
                     YTSIIEQTAKVFKYGTDDDSRIAEILAAGEELFGEDNVVEHHSNLDPDKEDARSRLAS
                     ENWDAPIIVTTNVQLFESLFACRSSQARKLHNIANSVIIIDEAQMLPPEFLKPILSVL
                     RGLVTHFGVTVVLCTATQPAFSGFVGSGEARFSGLEGCVEIMDDPNSLAKDFRRVSVC
                     FPSQEAPRREWEEIAEELQQHEQVLCIVNTRRDCRELHALMPKGTIHLSALMCGEERS
                     DIIADVKARLRRQGQEPVRVVSTQLVEAGVDIDFPVVYRALAGLDSVAQAAGRCNREG
                     RRKDLGRVVVFKPPRPAPPGFLRKGEDTCGEMLRTRSIEEIDPDLFTEYFRIFFNKAG
                     NFDKPQFEQRLLQEARDFKMQFRTIAGAFNLIDDAAQRSIIVQYEGSRRGVSSVSLIE
                     TLRRQGPSRWLSRKLQRFSVSVPKPIFDKIVNANMAEEVHGFFVQAGTGLYKPGLGLL
                     AEEKKWCEELLLV"
     assembly_gap    2126..2225
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2226..2554)
                     /locus_tag="PL162_07455"
     CDS             complement(<2226..2554)
                     /locus_tag="PL162_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HQF91888.1"
                     /translation="METPQEPIAHVKGNAREGWVPQPLGEHLEATARIAGEFAAAFGN
                     RDWGELLGFWHDLGKFVPDWQMHIRKGSGYDADAHIETYGGRVNHSSAGAVLAFERLK
                     GHPVARAL"
     gene            3083..4750
                     /locus_tag="PL162_07460"
     CDS             3083..4750
                     /locus_tag="PL162_07460"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HQF91889.1"
                     /translation="MLRAGATPVALKLAQRGGIGASVDRLVAWDQTECAVSPGVLAET
                     LVAAILCGHNPLTKLRDFWEGRECEPYFAAHGVRPEQLDDAAYGHLLDRIAEDRGRSL
                     VEAVALRYLVAHGMDAQLVHVSTASVSVGYDDGSEGDEDAGGPWEDEEIALLLAFGHR
                     RFKQTPYDPDDVDRWKIGLGVQQNGMPLTGKLLAGNVSDRAWSLDVARYAAHTLAAQG
                     LGHAVFVEDSALGAKGSLKNLVEEGLDFISILPGAFALEKELRDEAWHEDAWTTLEPF
                     GQEPQALQYQYWHTRRELVGEVLDFLVVTSSHPGERKEKILKTRFFELGEELKQEARA
                     LAEQEFAGEEEARRALERLKESGSTKGFQVSGQIRKSGALPEIGGTWRAVVSVGEVGE
                     ETYARARRQESTFVLAYRLKKNPAMNSPAEVLKAYKSQEMAEQGFRFLKQPVVLGPEF
                     QKTPERITALGYLFILPLILSQYLEYCVWTGLEKEGTMLILGGKKVFRPTTKIILECL
                     DRVMILQAEGQRFLTYGPQDNVMKILRALDVPSEVYTGRDGEDHVVR"
     gene            complement(4838..5776)
                     /locus_tag="PL162_07465"
     CDS             complement(4838..5776)
                     /locus_tag="PL162_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HQF91890.1"
                     /translation="MRAVRFVRGIALLVALFGLGMGEARAQETLAVTYVKAPLNIPSI
                     LERRLGLLDGAFSGDVTLSYPELTAGPQQTQAMAAGDVDIAHCLGSTSALLAAAGGVD
                     LRIVGIYSRAPEAFVILVPQTGGAASVAELKGKKVAGPKGTVLHQLLVGALRQAGLSP
                     ADVTFVPMGIPESVAALAGGAVDAALAAGPAVPKAEASGARILATGQGILEATTVIAA
                     RGELLRKHPERVTRFLEVHRQAVERITTHREEALRITAEEVGLSVEAVERMAALYDFD
                     PTIRPSDVEELRKTRDFLFENGLLTEKIEVETLLEK"
     gene            complement(5791..6600)
                     /locus_tag="PL162_07470"
     CDS             complement(5791..6600)
                     /locus_tag="PL162_07470"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009165716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQF91891.1"
                     /translation="MIGIRIRGLVKAFSPGGRTLRALDGVDLEIPPGSLTAIVGRSGC
                     GKTTLLRTLGGLETPDEGDVAFFDPSGPRDPRSLRRGMVFQEPRLFPWLSVRDNIAFS
                     LPREEGASAEAAERVEQILGKMRLQGFENALPHELSGGMAQRAALGRTLCFDPDLLLL
                     DEPFGALDYFTRRTLQHDLAALFRSEGKTVVLVTHDVEEALALGQRVLLLDRGRVLSS
                     WTLETPPGARAPDDPDVVRGRREILRHFGQSSGEGAGGRTEGSKILRNNTL"
     gene            complement(6597..7430)
                     /locus_tag="PL162_07475"
     CDS             complement(6597..7430)
                     /locus_tag="PL162_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQF91892.1"
                     /translation="MKPPVGACSLEGAAAPSVHVSPGALPRRRGTPLILPLLLLGLWQ
                     GASSLELWSSAVLPAPAAVGRALWGLAASGELFRHLGASFGRVLGGFALASALGCSLG
                     FLLGRRPALGALCGGTLEFLRHVPPLALLPMLILWYGIGEGSKVAVIVLASFFPVFLN
                     TLEGVTERDPRLEEVGRSLGLSEGEILRRIVLPEALPPVLVGLRLGLGYGWRSLIGAE
                     LIAAASGVGYLIQDAVALSRGDVVVAGILAMGGAGVALDALFAAVTERLVPWGGLRRS
                     S"
     gene            complement(7427..8692)
                     /gene="hydF"
                     /locus_tag="PL162_07480"
     CDS             complement(7427..8692)
                     /gene="hydF"
                     /locus_tag="PL162_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HQF91893.1"
                     /translation="MIDTPLAERVRIVLAGVRNSGKSALFNRLCDHDISIVSSKPGTT
                     GDPVTKGIEMGALGPVALVDTAGFDDDEDEVGSLRTGKTHGALALADAVVLVSRRDTP
                     PSEEERSFAARMAPGKKPFLLILTFGDRPADEEKLSWAAPFAPLTVANPSGEGVGTLR
                     QRLESLAAHLEPERDPLEGLVSSGDTVVLVTPIDASAPKRRLILPQVEVLRDLLDRHC
                     AAVVVQVPELAGAYGALTHRPQLVITDSQAFAEVASILPQDQPLTSFSILFARKKGDL
                     DIFIEGLHALGAMPPSCRVLILEACAHHRQDDDLGTVKIPALFRKLVCSGATFDFARE
                     LPPDLNEKSYDLVIHCAGCMVTRRQMLLRLERLRQVGIPSLNYGVFLAWAKGLIPRAL
                     EPLLPELAALPRDPEEGAPTLPEKGSAGA"
     gene            complement(8689..9786)
                     /gene="hydE"
                     /locus_tag="PL162_07485"
     CDS             complement(8689..9786)
                     /gene="hydE"
                     /locus_tag="PL162_07485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HQF91894.1"
                     /translation="MTSTVAPLFAPPPPAAPPEDLSRASADEVRNFFRHTSREELLRT
                     AAAVCQAHHGSRVLIRGLLEFSNVCARNCLYCGIREGNSRVRRYRLSEEDIRRTVRGA
                     FAVGYRTFVLQSGEDPSFSPARLCRLVEALREDLGASWALTLSCGIFPRQVYRDLFRA
                     GANRYLLRFETADPGLHRRLRGGVSLEERLQALRDLREEGYQVGSGYMVGLPGETDEG
                     FLANALLCRELELDMVGIGPFLPHPDTPLAASAAGTAERTLEALALVRLLLPRAHIPA
                     TTALATVHPGGRARGYEAGANVVMVNLTPGEVKGDYLLYPEKEGVHLDAPRALEAQME
                     ELGRCGRTGSLERGDALPRRSSSAQERKECS"
BASE COUNT         2028 a         3389 c         3401 g         1914 t
ORIGIN      
        1 cgcgggggga tgaactccat ggccgcatga atccggtacc gcacgtcgcg caggaacaac
       61 cccgcccgct gctgccgctg ctcttcgacg aaaaatccgt cgctgcggtc gctcatgaca
      121 agacccacct cattgcgccg tacggaaatc catttgatcg ggttcaacac ttcgattttt
      181 tgtactcgcc accgcactgc gggtttccag aacaccgctt cgaaaacacc ccgcgccgcc
      241 gaaggggtaa tgacgtcgta gctcacccgc tccaccttca tttccgggcg agtaaagcag
      301 gcgtagtccc cccaaacttc aagacagaag cttttgtcaa aaaacgcctt gctcacgagt
      361 acctcctttc cgcaccatgg ccggatcaaa ccaaaagcaa ctcttcgcac cacttttttt
      421 cttcggccaa caaccctaaa ccgggcttgt agagcccggt tcccgcctga acgaaaaagc
      481 cgtgaacttc ttctgccata ttcgcattga caattttatc gaaaataggc ttcggcacgc
      541 tcaccgaaaa tctctgcaat ttccgagaaa gccatcggct cggtccctgt cggcggagcg
      601 tttcgatcaa tgaaacgctg gagactccac gccgggatcc ctcgtattgc acgataatac
      661 ttctttgcgc cgcatcgtct atcagattga acgccccggc gatggtgcga aactgcatct
      721 taaaatcccg ggcttcttgg agtaaccgct gctcaaactg cggtttgtcg aaatttcctg
      781 ctttgttaaa gaatatccgg aaatattccg taaagagatc agggtcaatc tcctcgatgg
      841 agcgcgtccg cagcatttct ccgcaggtgt cttcgccctt gcgcagaaac cccggtgggg
      901 cgggtctagg tggcttgaac accaccacgc gccccagatc ctttcggcgt ccctcgcgat
      961 tgcaacgccc tgctgcctgg gccacggaat cgagccccgc taaggcacga tagaccacgg
     1021 gaaaatctat gtctactccc gcttccacga gctgcgtgct gaccacccgt accggctcct
     1081 gcccttgacg ccgtaaacga gccttcacgt cggcaatgat gtcgctgcgc tcctcgccgc
     1141 acatgagggc ggacaagtgt attgtgcctt tgggcatgag ggcgtgcagc tctcggcagt
     1201 ctcgccgggt gttcacgatg cagagtactt gctcgtgctg ctggagttct tcggcgattt
     1261 cttcccattc gcgccgtggc gcttcctgcg aaggaaagca caccgagacg cgacgaaagt
     1321 cttttgccag gctattgggg tcatccatta tctcgacgca tccttccaag ccagaaaatc
     1381 gggcttctcc ggacccgacg aagccggaaa aagcgggttg cgtggcggtg cacagcacca
     1441 cggtgacgcc aaaatgggtc accaacccgc gtaggacgga caaaatgggt ttgagaaatt
     1501 ccggcggcaa catctgggct tcgtcgataa tgattacgct attcgcgatg ttgtgaagct
     1561 tgcgcgcctg cgagctgcga caggcaaaga gggattcaaa aagctgcacg ttggtcgtca
     1621 cgatgatcgg agcgtcccag ttctccgagg caaggcggct tcgggcgtcc tctttgtcgg
     1681 ggtcgaggtt gctgtggtgt tccaccacgt tgtcctcgcc gaaaagctct tcgccagcgg
     1741 caagaatttc cgcgatgcgg gagtcgtcgt cggtaccgta cttaaagacc ttcgctgtct
     1801 gttcgatgat gctggtgtag gggatggcca tgatgatgcg acgcttgttg tgtgccaggg
     1861 cgtgttcgag ggcgaaagcc atggaagaaa gggtctttcc gcctccggtg ggcaccgtga
     1921 gggtataaaa tcccggcttt tctgcggctt tgcgccgaca ctgctccagg atttcccgtc
     1981 gtttgcgatt gatgagggag tcttctgcct cgcacacttt tcgctccatg taggcgtcaa
     2041 agcgatcttt caacgcggca agggatgcat gcccgccgcg actctccgcc ttctctggtt
     2101 ccatgaacat ctcggtgtcg agaaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnagggc tcttgccacg gggtggccct tgagacgttc gaaagccaac acggcaccgg
     2281 cggagctgtg gttcacacga cctccatatg tttcaatgtg ggcatcggca tcgtatccgg
     2341 aacccttgcg gatatgcatt tgccagtcgg ggacgaattt ccccaggtca tgccaaaaac
     2401 ccaacaattc tccccagtcc cggttgccaa aagcggcggc gaactctccc gcgatccgag
     2461 ccgtggcttc cagatgttct ccaagaggct ggggaaccca cccttctcgc gcgttgccct
     2521 tcacatgggc gatgggttct tgtggcgtct ccatcagcaa ccccgtcctt tctttagata
     2581 cactcccccg tatgcggtga tctaaaagct ccgactccga gaacgtcctc ctgcttctcc
     2641 gtagtaattt tgattatagc agggaggtcc gacatcataa gtgggtcctg cgccgccacg
     2701 caaggttttt tcgagaatct tttggcgtta gatgtgttga gggggaggtg tggtgtataa
     2761 aattcataaa accaatttaa cgcgctttgc atccattgtc acgcccttct cggtaccttc
     2821 cgtgaccaag cattaatgtg aaaagctccc ggtttctggc acaccttcct ctgcggcaca
     2881 aaactacata gggcttcccc ccgttcgacg agaagctccc ttctcgccct ccctataagg
     2941 gattgaaacg gcccccccct ctcgtacaag gccgcaggga ccggtggggg ggccctcgca
     3001 cggtgacacg ggggcccatc tcggctagca tacggccatg aaaaacatga atgacatctt
     3061 tgtttccctt tcgtcggctc aaatgctgcg cgctggtgct acgcctgtcg ccttgaaact
     3121 cgcccaacga ggaggaatcg gagcatcggt ggacaggctc gtggcatggg atcagacgga
     3181 gtgcgcggtt tctcccggcg ttctggccga aacgttggtg gcggcgatcc tctgtggcca
     3241 caaccccctg acaaagcttc gggacttttg ggaagggcgc gaatgcgaac cctactttgc
     3301 cgcccacggc gttcgtccgg aacagctcga cgacgccgca tacggccatc tccttgacag
     3361 gatcgccgaa gaccgtggaa gatccctggt cgaagccgtg gcgttgcggt accttgtggc
     3421 gcacggcatg gacgcccagc tggttcatgt cagtaccgcc tcggtctcgg tggggtatga
     3481 cgacggatcg gagggcgacg aggacgccgg gggtccttgg gaggacgaag agatcgccct
     3541 cttgttggcc ttcgggcaca gaagatttaa acaaacgccc tatgaccccg atgacgtgga
     3601 ccgctggaag atcggcctcg gggtgcagca aaacggcatg cctttgacgg gaaaactttt
     3661 ggccgggaat gtttcggatc gcgcctggag tctcgacgtg gcgcggtacg ccgcgcatac
     3721 cctcgcggcc caggggttgg gccatgcggt tttcgtggag gacagcgccc ttggggcgaa
     3781 gggaagcctc aaaaatctgg tggaggaggg gctcgatttc attagcattc tccccggggc
     3841 gtttgccctg gagaaagaac tccgggacga agcgtggcac gaggacgcct ggaccaccct
     3901 ggagcccttc ggccaagagc cccaggccct ccagtaccaa tattggcaca cccggcgcga
     3961 gctggtcgga gaggtccttg acttcctcgt ggtgacctct tcccacccgg gggaacgcaa
     4021 agagaaaatc ttgaagacgc gttttttcga gctcggagaa gagttgaaac aagaggcccg
     4081 ggccctggcc gagcaggagt ttgctggcga agaggaggca agacgggccc tggagcggct
     4141 gaaggaatcc ggaagcacga agggcttcca ggtctcggga cagatccgga aaagcggtgc
     4201 gcttccggag atcggcggaa cgtggcgtgc cgtcgtcagc gtgggggagg tgggtgagga
     4261 gacctatgcc cgggctcgcc gtcaggagtc cacgtttgtc ctggcctatc gcctcaagaa
     4321 aaaccccgcc atgaactctc cggcggaagt cctgaaggcg tacaagagcc aggagatggc
     4381 ggagcagggc tttcgcttcc tgaagcaacc cgtagttctg ggccccgaat ttcagaaaac
     4441 tccggagcgc atcacggctc tgggatacct gttcattctt ccgctgatcc tgtcgcaata
     4501 tctggagtac tgtgtgtgga cggggctgga aaaagaggga accatgctta tcctgggagg
     4561 aaaaaaggtt tttcgtccca cgacaaaaat cattctcgaa tgtcttgatc gggtgatgat
     4621 cctgcaggcg gaggggcaac gctttctgac ctacgggccg caggacaatg tgatgaaaat
     4681 tcttcgtgcc ctggatgttc cttcggaagt gtacaccggg agagatggcg aagatcacgt
     4741 tgtgaggtaa aattccccga aaccccaggc cccgggcttc cggcgtacca tgccgggccc
     4801 ggggcctgtg ttgtgtggat ttcccgagga acgcccccta cttttccagg agcgtttcca
     4861 cctcgatctt ttccgtcagc aggccgtttt caaagagaaa atcccgggtc ttgcggagct
     4921 cttccacgtc gctggggcgg atcgtggggt cgaagtcgta gagggccgcc atgcgctcca
     4981 ccgcttccac ggagagcccc acttcctccg cggtgatgcg aagggcctct tcccggtggg
     5041 tcgtgatgcg ctccaccgcc tgccggtgca cttccaggaa gcgcgtcacc cgttccggat
     5101 gcttccggag gagttccccc cgggcggcga tgaccgtggt ggcttccagg atgccctggc
     5161 ccgtggcgag aatccgggcc cccgaggcct ccgccttggg gacggcgggc cccgccgcca
     5221 gagccgcgtc cacggcgccc cccgccaggg cggcgacgga ttcggggatg cccatgggga
     5281 caaaggtcac gtcggcgggg gagagccccg cctgccgaag ggcccccacc aggagctggt
     5341 ggagcaccgt gcccttgggt cccgccactt tcttgccttt cagctccgcc accgaggcgg
     5401 ctcctcccgt ctggggcaca aggatcacga aggcctcggg ggctcggctg taaatgccca
     5461 cgatccgcag atccacgccc cccgccgccg ccagcagggc ggacgtgctc cccaggcagt
     5521 gggcgatgtc cacatccccc gccgccatgg cctgggtctg ctgggggccc gccgtgagct
     5581 cggggtagga gagggtgacg tctccggaga aggctccgtc cagaagcccc agacggcgct
     5641 ccagaatcga agggatattc aggggtgcct tgacataggt caccgccagc gtctcctggg
     5701 cccgggcctc gcccattccc agaccgaaga gggccaccaa gagggcgatt ccccgaacaa
     5761 atcgtacagc gcgcatgcaa aaaacctcct tcaaagggtg ttgttccgca aaattttaga
     5821 accttccgtc cgacccccgg ctccttcgcc ggagctctgg ccgaaatgcc gcagaatttc
     5881 ccgccgcccc cggaccacgt cggggtcgtc gggggcccgg gcccccggag gggtttccaa
     5941 ggtccacgag gagagcaccc gcccccggtc caggagcagc acccgctgcc ccaacgccag
     6001 ggcctcctcc acgtcgtggg tgaccagtac cacggtcttg ccttcgctgc gaaacagggc
     6061 ggccagatcg tgttgcaggg ttctccgggt gaagtagtcc agggcgccga agggctcgtc
     6121 caggagcaaa agatcggggt cgaaacagag ggtgcgcccc agggcggccc gctgggccat
     6181 gccgccggaa agttcgtgag ggagggcgtt ctcgaacccc tgaaggcgca tctttcccag
     6241 aatttgctcc acccgctccg ccgcttcggc cgaggccccc tcctcccggg gcagcgaaaa
     6301 ggcgatgttg tcccgcaccg aaagccaggg aaagagccgg ggctcctgaa agaccatgcc
     6361 ccggcgcaac gaccggggat cccgggggcc cgaggggtcg aagaacgcca catctccctc
     6421 gtccggggtt tccagccccc ccagggtccg cagcagggtg gtcttgccgc aaccgctgcg
     6481 ccccacgatg gcggtgaggc tccccggggg gatctccaga tccacgccgt ccaaagcccg
     6541 gagggttctt cctccggggg agaaggcttt cacgaggccc cggatccgga tgccgatcac
     6601 gacgacctcc ggagaccgcc ccagggcaca aggcgctcgg tgacggcggc gaagagggca
     6661 tccagggcca cccccgcccc gcccatggcc agaatgcccg ccaccaccac atctccccgg
     6721 gagagggcca cggcatcctg aatgaggtac cccacccccg aggcggcggc gatgagctcc
     6781 gcgccgatga gggagcgcca gccgtacccc agcccgaggc gcaaccccac cagcacgggg
     6841 ggaagggcct cggggagcac gatgcgccgc aggatctccc cctccgaaag ccccagagag
     6901 cgccccactt cctccagccg gggatctcgt tccgtcaccc cctccagggt gttgaggaac
     6961 acgggaaaga aggacgccag cacgatcacc gccactttgg agccctcgcc gatgccgtac
     7021 cagaggatga gcatggggag aagggctagc gggggcacgt gccgcaaaaa ctccagggtg
     7081 ccgccgcaga gggcccccag ggcggggcgc cgtcccagaa gaaagcccag ggaacacccc
     7141 agggccgacg ccagggcaaa gccccccagc acccggccga agctcgcccc caggtggcgg
     7201 aagagctcgc cgctcgccgc gaggccccaa agggcccgcc ccacggcggc gggggcgggg
     7261 agcacggcgg agctccagag ctccagcgag gaggccccct gccagagccc cagaaggagc
     7321 aggggaagga tcaggggcgt tccccggcgg cggggaaggg ctccggggga aacgtggacg
     7381 gagggggcgg cggccccttc cagagaacag gcgcccaccg ggggtttcat gccccggcgc
     7441 ttcctttctc cggcagcgtg ggggcccctt cttccggatc ccggggcagg gcggcgagct
     7501 ccggcaacag gggttccagg gcccggggga tgagcccctt ggcccaggcg agaaagaccc
     7561 cgtagttcag cgaagggatc cccacctgac ggaggcgttc gaggcgcaaa agcatctggc
     7621 gccgggtcac catgcacccc gcgcagtgga tcaccaggtc ataggacttc tcgttcagat
     7681 ccgggggcag ctcccgggca aagtcgaagg tggccccgga gcacaccagc ttccggaaga
     7741 gggcgggaat tttcacggtg ccgaggtcgt cgtcctggcg gtggtgggcg caggcctcca
     7801 ggatcagcac ccggcaggac gggggcatgg cgcccagggc gtggagtccc tcgatgaaga
     7861 tgtccagatc gcccttcttc cgggcgaaga ggatggaaaa ggaggtgagg ggctggtcct
     7921 gggggaggat cgacgccacc tcggcaaagg cctggctgtc ggtgatcacc agctgggggc
     7981 gatgggtcag cgccccatag gctccggcga gttcgggcac ctgcaccacc accgcggcgc
     8041 agtgtcggtc cagaaggtcc cggagcacct ccacctgggg aagaatgagg cgccgcttgg
     8101 gggcggaggc gtcgatgggg gtcaccagca ccaccgtgtc ccccgaagac accagcccct
     8161 ccagggggtc ccgctcgggc tccagatggg cggccaggga ttccaggcgc tggcgcaggg
     8221 ttcccacccc ctcgccggaa ggattggcca ccgtgagggg ggcaaagggg gcggcccagg
     8281 agagtttttc ctcatctgcg gggcggtcgc cgaaggtgag gatcagcaga aagggctttt
     8341 ttcccggggc catgcgggcg gcgaagctcc gctcctcctc gctcgggggg gtgtcccggc
     8401 gggacaccag caccacggcg tccgccagcg ccagggcgcc gtgggtcttc cccgtgcgaa
     8461 ggcttcccac ctcgtcttcg tcgtcgtcaa agccagcggt gtccaccagg gccacgggcc
     8521 ccagggcccc catctcgatg cccttggtga ccggatctcc cgtggtgccg ggcttggagg
     8581 agacgatgga aatgtcgtga tcgcagaggc ggttgaacag ggcggatttt ccgctgttgc
     8641 gcacccccgc caggacaatg cggacccgtt ccgccagcgg cgtgtcgatc atgagcactc
     8701 cttcctttct tgtgcggaag agcttctgcg ggggagggcg tctccccgtt ccagcgaccc
     8761 cgtccggccg cagcgcccga gctcttccat ctgggcttcc agcgcccggg gggcgtccag
     8821 atgcacccct tctttttccg gatagagcag gtagtctccc ttcacctcgc cgggggtgag
     8881 gttcaccatc accacgttgg cccccgcctc gtacccccgg gcccgccccc ccgggtgcac
     8941 cgtggcgagg gccgtggtgg cggggatgtg ggcccggggg agcaacaggc gcaccagggc
     9001 cagcgcttcg agggtccgct ccgccgtccc cgccgccgat gccgccaggg gcgtgtcggg
     9061 gtggggcaaa aaggggccga tgcccaccat gtccagctcc agctcccggc agagcagggc
     9121 gttggcgaga aatccctcgt cggtctcccc cggaagcccc accatgtacc ccgatcccac
     9181 ctggtatccc tcttctcgga gatcccggag ggcttgaaga cgctcttcca gcgagacccc
     9241 tccccgaagg cgccggtgca gcccgggatc cgccgtctcg aaccggagca gatagcggtt
     9301 ggcccccgcc cggaagagat cccggtaaac ttgccgggga aagatgccgc agctcagggt
     9361 gagggcccag ctcgccccca gatcttcccg cagggcttcc accaggcggc acaggcgggc
     9421 gggggaaaag gaggggtcct cgccgctctg gagcacgaag gtgcggtatc ccacggcgaa
     9481 ggccccccgc accgtgcgcc ggatgtcttc ttctgaaagg cggtagcgcc ggacccggga
     9541 gtttccctcc cggatgccgc agtagagaca atttcgggcg cagacgttgg aaaactccaa
     9601 caacccccgg atcagcaccc gggagccgtg atgggcctga cacactgcgg cggcggtgcg
     9661 caaaagttcc tcccgggagg tgtgacgaaa gaagtttcgc acctcgtcgg cggaggcccg
     9721 ggagagatct tccgggggcg cggccggagg cggcggggca aaaagtggtg cgacggtgga
     9781 tgtcatggag cgtcctttct ccccggagcg ggggatcttt ggcaaacctt cgggcccgtc
     9841 aaaaatcccc ttccggacag ggggccggtt tatggggcga aactcgggcg ccggaggacg
     9901 ggcagttttg ataccccccc gggtctttaa aatatatcac acgtcagcga ggcctctgac
     9961 agcataccac ccggaggggc ggagcgcacg atgggccccg ggcgaaaagg gaggaaaaat
    10021 ggggcaaaag gggagaaaac ttcctccgga gggggatatc cgtatatttg gaactctttt
    10081 actagaatag taccccaagc ccccctccgc aagggggaac gcatcgggac cttccacaag
    10141 ccggaaccac gctgtagggg cgaaaaacac gctcgcctct actaccgcga agaaaaacgg
    10201 aaaccgcaac ggtgagctcc gatgccaacg gggactttac cctacgcgac atcaaggccc
    10261 tttcccggcg gggagaattc ctctctgcag ggatttttgt gctcctgagg cagcgagccc
    10321 ggagcggagg gagccccgcg cgtgtcttgg ttccgcccct ccggcgggag gatccccagg
    10381 aggaaaaaga ttttctcggg gaattctccg ggggagatgc cttgatccgg ccgacgcaga
    10441 ctgccccaag gctcgttctg gcgcttcggg tgagggatac cagtacattg cgagggggtc
    10501 ggacacgccc ctgggctccc ggcccttccc catagggcca cgggcaaatc tttttcgctc
    10561 accctatcct atgctgttcg ccagaccacc tgtgagcggg ggccacaggt cccctgctgc
    10621 tgctccctgc ggcgcttctg ctaggttgtg ttctcaaaac ttgaaattga gtccgcaata
    10681 gtttatcatg cggtcttatg agccgacatg atatcagtga tgagatgtgt aatcgtcttt
    10741 ctcctttgtt tccgccggaa cgaagcggca aacaggagag agcgtgccac gacaaccgga
    10801 ggatcgtaaa cggtttgtcg tggatccttc gg
//
LOCUS       DAOOER010000057        10742 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_6644_length_10742_cov_6.858988, whole genome shotgun sequence.
ACCESSION   DAOOER010000057 DAOOER010000000
VERSION     DAOOER010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 10742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10742
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6644_length_10742_cov_6.858988"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..879)
                     /gene="hisF"
                     /locus_tag="PL162_07490"
     CDS             complement(88..879)
                     /gene="hisF"
                     /locus_tag="PL162_07490"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HQF91895.1"
                     /translation="MLTKRIIPCLDVKDGRVVKGIRFRELRDAGDPAHLAGVYQEEGA
                     DELVFLDISASTERRSTRRDWVASVADRLSIPFTVGGGISSEEEAVELVALGADKISL
                     NTAAVRRPELFSECARRLGQQAVVAAVDVCRRDDGWEVIIEGGQTPTGLDALAWVREA
                     TARGAGEILLTSMDRDGTASGYDRELLVAVCAAVSVPVIASGGAGTVAHLAEAFEAGA
                     EGALAASIFHYGTLRIGEVKEALAARGIPVRRGGSGPTGENRPWA"
     gene            complement(986..1702)
                     /locus_tag="PL162_07495"
     CDS             complement(986..1702)
                     /locus_tag="PL162_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HQF91896.1"
                     /translation="MKILPAIDLYEGRVVRLRQGDFDRCTAYGDDPCAVAASFRDAGA
                     REIHVVDLEGARQGAPVHLSLLAPLRALGVEVRYGGGLRTAEDGDRALAAGATRIMVG
                     SLLFRDPLQTAALGRRFGKALVASVDVKEGRVAVGGWTATVDRPPAEALEELAAAGCD
                     EFLVTAVSRDGTGEGPDFDLYRGLRSRCPHVALVAAGGIAHLDDLKGLHALGLAGAVV
                     GRALYEGTVDLAEALRTFPP"
     gene            complement(1794..2363)
                     /locus_tag="PL162_07500"
     CDS             complement(1794..2363)
                     /locus_tag="PL162_07500"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase"
                     /protein_id="HQF91897.1"
                     /translation="MNHVKRITRETSVELSLELAPGDGVIETPCGFLNHMITLLAHHA
                     GWQVRLAASGDLAVDAHHLTEDVGIALGQALLQELQSGPPRRRYGECMLPMDGTLVLA
                     AVDLSGRGFLAWEGTFPSPRCGDFDTELVPEFWRAFARESRSTIHLRALAVDNSHHLA
                     EAAFKGAGRALRGALCPGDTPPSTKGVWL"
     gene            complement(2424..3059)
                     /gene="hisG"
                     /locus_tag="PL162_07505"
     CDS             complement(2424..3059)
                     /gene="hisG"
                     /locus_tag="PL162_07505"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HQF91898.1"
                     /translation="MLTVALPTGRVMEEARTLLKNLGLPTEALGQGGRDLVVEEGTMR
                     YLLAKPTDVPVYVLWGAADLALAGSDVLRESGAPLVELGDTGYGRCRLAVAGPPAISA
                     LFSGAGNRRQGLRVATKYPRIAEGHFSARGTQVEIVPLHGSIELAPRLAMADCILDIV
                     QTGSTLRANGLVVLEDVGPVSLRMVASPRSARLRWDLIAPLVRAMETHRKR"
     gene            complement(3053..>3933)
                     /locus_tag="PL162_07510"
     CDS             complement(3053..>3933)
                     /locus_tag="PL162_07510"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HQF91899.1"
                     /translation="ERFQVGAELLGWEGHGADAELLALLMRSLARMGATGCTAVLGDV
                     TLVEEALEALPAPIGAALGEALRRQDLTTYQGIMARAELPAERRPLLEELPWLKGDRT
                     VLDRAVRLGVAPSRVAPLHFLAETLESLECGADLRFDLALFRELGYYSGPVFEIYAPC
                     GGRPLGGGGRYDGLLASHGLLGQALGVALDLEALAEATLEKKEGRPGVMVWGGSGTSA
                     HTMKVAELLVGRGWTVELSWIEDATASRSLARIKKFRWWVDGAAESVTFLRNSHTVPL
                     ERWLAKPEDQGEDGSC"
     assembly_gap    3934..4033
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4155..5408)
                     /gene="hisD"
                     /locus_tag="PL162_07515"
     CDS             complement(4155..5408)
                     /gene="hisD"
                     /locus_tag="PL162_07515"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HQF91900.1"
                     /translation="MRYLKSRAAPPSSSESSVAAEVAPLVARLRHRGWEAVEELSRSL
                     DHFEGPYRVPVEVLRRSADGLSPALREALLTAEENVRAFCRYQRSLLADREWELSPGV
                     RAGVRFLPVRRGAVYVPGGRYPLPSTAIMGVVPAQEAGVERIVALSPPRGAAGVDPVV
                     LGTLGLLGVEEVWALGGVQGVGALALGLGPVERADLLVGPGNAWVTEAKRQFFGELGL
                     DGLAGPSEVCIVADATADPATLAADLLAQSEHDPLAQGVLLALDEELGQKTLDEVQRL
                     LDALPTAAVARSSWDRFGVVAVATLEEATAFANQLAPEHLELALESPRSALPLFRNYG
                     AAFLGHASAEAFGDYVAGTNHILPTGGRARFSGGVWVGTFLRPLTHLELSPEAAERLA
                     PPGETLAAAEGLSAHGAALSARRKP"
     gene            complement(5591..6631)
                     /locus_tag="PL162_07520"
     CDS             complement(5591..6631)
                     /locus_tag="PL162_07520"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HQF91901.1"
                     /translation="MPPSQVSPKTIWTVALHLLLIGGIIAVVYRTGDVMTWIVLSAFL
                     AVALNAGVLGLERRGLRRRWAIGVVFILAGSAIAVAVSTIIPLVVSQIAALVRVAPDA
                     IAKLENLAAVQALEEEYHVFAQLRGWASQNAPHLAQAAVSVVTGALSGVLSLGMVLVL
                     TVFMLLCGNEFTEDLLAFVAERRRAGVRSALRTMQTTVSAYVAGTVFIALVGGGVIGT
                     ALLLAGSPYYLALGFCTALLGLLPYLGPVLAAVLVVSTTFSTLGSWPATALLGVFLIY
                     GAIESNVLQPVVQSRSIRMNPLLVFLVMLVGTSLAGMMGALLALPVAGATQAFVKQVL
                     LRENPMEDDAPA"
     gene            6861..8210
                     /gene="radA"
                     /locus_tag="PL162_07525"
     CDS             6861..8210
                     /gene="radA"
                     /locus_tag="PL162_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HQF91902.1"
                     /translation="MAKKNETRFVCTSCGAPSSSWAGKCPRCGAWGSLEQDFAPRTAP
                     GGGKAPGGVPQARAVRASEVPLLDRIPTGLDELDRVLGGGFAPGSVSLLAGEPGIGKS
                     TLLLQACLALARRGRTVLYVSGEESLEQVASRALRLDGQPPPLDLLASGDPEEFMDLL
                     PGRSLVVVDSVQAMGDGESLPGTLSQVRGVAARCIEGAKRSGVPLVLVGHVTKDGQIA
                     GPKLLEHMVDVVLSFEGDRHTAYRILRSSKNRFGSTDEIGVFEMGEEGLRPVADASLL
                     FWNGAEGGVPGVAFGVTLEGSRPFVVELQALACPTPFPYPKRTARGVEVNRVQLLLAV
                     LEKRCGLSARTADAYVNVAGGLAVRDPGADLALCAALASSLLDRPLSAGGCLVGEVGL
                     AGEVRPCPRTGARLHEAARLGFTRGVVSSFEEKNFDGELEVVRVGSLSEALAALFPR"
     gene            8264..9562
                     /locus_tag="PL162_07530"
     CDS             8264..9562
                     /locus_tag="PL162_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HQF91903.1"
                     /translation="MCLALALLWAGKAAAESLVMVTPLRGAVGAALEHHLEEVIQECD
                     KEGVALLVLEIDTPGGLVSSMREMVRMIQNAPCPVVAWVAPGGAQAASAGAFLLEAAH
                     VAAMAEGTNVGAAHPVGVGGGEGGEMDRKITNDLTAQMRSLAQLRGRNADAAARMVTE
                     SASYPSREALEGGVVDLVVGDLAALISSVEGRIVTMPQKEVVLSVGAYRVKARPMSLP
                     LRILEVITRPDVAYLLLVAGIYALIFEALTPGGFVMGISGALLVLLGSLGMRMLPFSW
                     AGILLLVAGVGVMILDLVVGGMGFLSLFGAAALVVGGLVIFRAPGGELMRFSMEVMGG
                     GVLGISLFFLLSVWAVWRAMRHRAVSGQEGLVGARGRTLQELNPQGMIKCHGEYWKAR
                     TESGLKIPDDTPVEVVAMEGLTLVVRPQSPEGADGAKSAK"
     gene            9580..10365
                     /locus_tag="PL162_07535"
     CDS             9580..10365
                     /locus_tag="PL162_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HQF91904.1"
                     /translation="MDLLMDVLFSILSNFSALLILVFMLVSAIKVVPEYQRLVLFRLG
                     RLVGSRGPGLVLVIPMVDRTARVDLRVVTLDVPVQEVITQDNVPIKVNAVVYFRVMDP
                     SRSVVEVENYIMATSQLSQTTLRSVIGRAELDEVLSDRDKINQELQQIIDERTDPWGI
                     KVSAVEVKELELPEGMKRAMASQAEAERDRRAKIINAEGELQASQKLLEAAQVMERAP
                     ISLQLRYLQTLREIASEKNSTTIFPLPIDLVKPFLDAYLKPRG"
     gene            10480..>10742
                     /locus_tag="PL162_07540"
     CDS             10480..>10742
                     /locus_tag="PL162_07540"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HQF91905.1"
                     /translation="MRILAINGSPRGAQGNTEAMLQPFLEGARAFGAETEVVYARDLS
                     VGFCQGCFACWFKTPGRCILKDDMEALLERLRRADAVIWATPL"
BASE COUNT         1765 a         3504 c         3721 g         1652 t
ORIGIN      
        1 tgagcacggc gccggactgg gcgtcttgga ccaccacggg cacgaggccc tgcgggtcga
       61 agcgcagcgt ttcaaggccc gtgagcgtca ggcccatggc cggttctccc cggtggggcc
      121 ggagccgccg cgtcgtacgg ggattccccg ggcggcgagg gcctctttta cctcgccgat
      181 gcggagggtg ccgtagtgaa agatggaagc cgccagggct ccttcggccc ccgcctcgaa
      241 ggcctcggcc aggtgggcca ccgtccccgc tccgcccgag gcgatgacgg ggacggaaac
      301 ggcggcgcag acggcgacga ggagctcccg gtcgtatccg gaggccgtgc cgtcccgatc
      361 catggaggtg aggaggattt cccccgcccc ccgggccgtg gcctcccgga cccacgccag
      421 ggcgtccagt cccgtgggag tctgcccccc ttcgatgatc acttcccacc catcgtcccg
      481 ccgacagacg tccaccgccg ccaccacggc ctgctggccc aggcggcggg cgcactccga
      541 aaagagctcc gggcgccgca ccgccgcagt gttcagcgat attttgtccg cccccagggc
      601 cacgagctcc accgcttctt cctccgaaga gatgccccct cccacggtga aggggatgga
      661 gagccggtcc gccaccgatg ccacccaatc cctccgggtg ctccggcgct ccgtcgaggc
      721 ggagatgtcc agaaagacca gctcgtctgc cccttcctcc tgataaaccc ccgcgaggtg
      781 ggcggggtcc cccgcgtccc ggagctcccg gaagcggatg cccttgacca cccgtccgtc
      841 cttgacgtcc agacagggga tgatgcgctt ggtcaacatg aaagtacctc ctgctaaagt
      901 atagaatttt ttcggagcgc cagggggcct ccggaacgga tttaatctta tatcaacgac
      961 catcggtatg ggcttaaggg agggcctacg gagggaaggt tctcagggcc tcggcgaggt
     1021 ccacggtgcc ttcgtagagg gcgcgcccca ccaccgcccc cgccaggccc agggcgtgga
     1081 gccctttgag gtcatccagg tgggcgatgc ccccggcggc caccagggcg acgtgggggc
     1141 atcgggaccg caacccccgg tagaggtcga agtcggggcc ttcccccgtg ccgtcccggg
     1201 atacggcggt gacgaggaac tcgtcgcatc cggcggcggc gagctcttcc agggcttctg
     1261 cgggggggcg atccaccgtg gcggtccatc cccccacggc cacccgccct tccttcacgt
     1321 ccacggaggc caccagggcc ttgccaaagc gtcgccccag ggcagcggtc tggaggggat
     1381 cccgaaagag caggcttccc accatgatgc gggtggcccc cgccgccagg gcccggtctc
     1441 cgtcctcggc ggtgcgcaac cctccgccgt agcgcacctc cacccccagg gccctcaggg
     1501 gcgccaaaag agaaaggtgc acgggggcac cctgccgggc cccttccagg tccaccacgt
     1561 ggatttcccg ggcccccgcg tcccggaagg acgccgccac ggcgcacggg tcgtccccgt
     1621 aggcggtgca ccggtcaaaa tctccctggc gcaggcgcac cacgcgcccc tcgtagagat
     1681 cgatggcggg caggatcttc atgacgcgcc cccggccatg ggcgaggccc ccgggcggcg
     1741 cagggcccgc tccgggtttc ccccgttccc ggtgccgtat tccacgacgc cgatcacagc
     1801 cacaccccct tggtgctcgg gggggtgtcg ccggggcaca gggccccccg cagggcccgt
     1861 cccgcgccct taaaggccgc ctcggcgagg tggtgcgagt tgtccaccgc cagggcccgg
     1921 aggtgtatgg tgctccggga ttcccgggcg aaggcccgcc agaactcggg caccagttcg
     1981 gtgtcgaaat cgccgcaccg gggggagggg aaggtcccct cccaggccaa aaacccccgg
     2041 ccgctgaggt ccaccgccgc caggaccagg gtgccatcca tgggaagcat gcattcgccg
     2101 taccgccttc gcgggggtcc ggattgcagc tcctggagga gggcctgccc cagggcgatc
     2161 cccacgtcct cggtgaggtg atgggcgtcc acggccagat ctcccgaggc cgcgaggcgc
     2221 acctgccacc ccgcgtggtg ggccagcagg gtgatcatgt ggttaagaaa gccgcagggc
     2281 gtttcgatga ccccatcgcc gggggccagc tccaaggaga gctccacgga ggtctctcgg
     2341 gtgatgcgct tgacgtgatt caaaagaatc ctcctcgtcc gggcgcctca ggcgccccgt
     2401 cgtcggccct ttaagggttt ttatcagcgt tttctgtgcg tttccatcgc ccgaaccaag
     2461 ggggcgatga ggtcccaccg caggcgggcg ctccgggggc tggccaccat gcgcagggat
     2521 acggggccca cgtcctccag caccaccagg ccgttggccc gcagggtgga gccggtctgg
     2581 acgatgtcca ggatgcagtc ggccatggcc aggcgagggg ccagctcgat ggagccgtgg
     2641 agggggacga tctccacctg ggtgccccgg gcggagaagt gcccctcggc gatgcggggg
     2701 tacttggtgg ccacccgcaa cccctggcga cgatttcccg ccccggaaaa gagggccgag
     2761 atggcggggg ggcccgccac cgccagacga catcggccat accccgtatc ccccagctcc
     2821 accaggggcg cccccgattc ccgcaaaacg tcgcttcccg ccagggcaag gtcggcggcg
     2881 ccccagagga cgtagacggg gacgtcggtg ggctttgcca agaggtagcg catggtgcct
     2941 tcttccacca ccaggtcccg tcccccctgc cccagggcct cggtggggag tcccaggttt
     3001 ttgagcagcg tccgggcttc ttccatcacc cttccggtgg gcagggccac ggtcagcatg
     3061 atccatcctc cccttgatct tccggtttgg ccagccatcg ttccaggggc accgtgtggg
     3121 agttccggag gaaggtgacg ctctccgccg ccccatccac ccaccagcga aacttcttga
     3181 tccgggccag ggaccgagag gccgtcgcgt cctcgatcca gctcagctcc acggtccatc
     3241 cccggcccac gaggagctcc gccactttca tggtgtgggc cgaggtgccg gagccccccc
     3301 ataccatcac gccggggcgc ccctctttct tttccaaggt agcctccgcc agggcttcca
     3361 ggtccagggc cacccccagg gcctgcccca gcaggccgtg ggatgccagc aggccgtcgt
     3421 agcgcccccc accccccagg ggacgtcctc cgcagggggc gtagatctcg aacaccgggc
     3481 cgctgtagta ccccaactcc cggaagaggg ccaggtcgaa gcgaaggtcc gccccgcact
     3541 ccagggattc gagggtttcg gccaaaaaat gcagcggggc gacccgggag ggggcgaccc
     3601 cgaggcggac ggcccggtcc agcaccgtgc ggtccccctt gagccagggg agctcctcca
     3661 gcagggggcg cctctcggcg gggagctcgg cccgggccat gatcccttgg taggtcgtga
     3721 ggtcctggcg ccgcagggcc tcccctaaag cggctccgat gggggcgggg agggcctcca
     3781 gggcctcttc caccagcgtc acgtccccca gcaccgccgt acaccccgtc gcccccatgc
     3841 gcgccaggga tcgcatgagg agggccagga gctccgcatc ggccccatgc ccctcccacc
     3901 ccaggagctc cgcccccact tggaagcgct ccannnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnncccgagg ggaaaaagag gcgatagccg aagctcctaa agacgtcgag
     4081 gaaggtgtcc cgggcccccg ccatggcctc ggcgcgccct ccccccaccg tgcgacaccc
     4141 cggaagcgcc cggatcacgg cttcctccgg gccgaaaggg ctgcgccgtg ggccgaaagc
     4201 ccctccgccg ccgccagggt ctcccccgga ggggcgaggc gctccgccgc ttcgggggag
     4261 agctccaggt gggtgagggg gcggagaaag gtccccaccc agactccgcc ggaaaagcgg
     4321 gcccggcccc ccgtggggag gatgtgattg gtccccgcca cgtagtcccc aaaggcctcg
     4381 gcgctggcgt gccccagaaa ggccgcgccg tagtttcgaa acaggggcag ggcgctccgg
     4441 ggggactcca gagccagctc caggtgttcc ggggccagct ggttggcgaa ggccgtcgct
     4501 tcttccaggg tggccaccgc caccacgccg aagcgatccc aggagcttcg ggccaccgcc
     4561 gccgtgggca gcgcgtccaa aagcctttgg acctcgtcca aggtcttttg tcccagctct
     4621 tcgtccagcg ccaggagcac cccttgggcc agcgggtcgt gctccgactg ggccaaaagg
     4681 tccgccgcca gcgtggcggg gtcggcggtg gcatcggcca cgatgcagac ctcgctgggg
     4741 cccgccaggc cgtcgaggcc caattccccg aaaaactgcc gtttggcctc ggtgacccag
     4801 gcgtttcccg gccccaccaa aagatcggcg cgttccacgg gccccaagcc cagggccagg
     4861 gcccccaccc cctggactcc ccccagggcc cagacctctt ccacccccag aagccccagg
     4921 gtgccgagca ccacgggatc cacccccgcc gctccccggg gaggagagag ggccacgatg
     4981 cgctccaccc ccgcctcctg ggcgggcacc acccccatga tggcggtgct gggaaggggg
     5041 tagcgccccc cggggacgta caccgccccc cgtcgtacgg gaaggaatcg cacccccgcg
     5101 cgcacccccg gagagagctc ccactctcgg tcggcgagga gggagcgctg gtatcggcag
     5161 aaggcgcgga cgttttcctc cgccgtgagg agggcttccc tcagggcggg ggagagccca
     5221 tcggcggagc ggcgcaggac ttcgacgggg acgcgatagg gcccctcgaa gtggtccagg
     5281 gaccgggaaa gctcttccac ggcctcccat cctcggtggc gcaatcgcgc caccaagggg
     5341 gccacttcgg cggccaccga gctctccgag gatgaggggg gagcggcacg gctcttgagg
     5401 taacgcaggg gaatcccttc tttctaatat caagataaag tggcattccg aacaaacgag
     5461 aggatgatag caaaatagtc cggggagggc aagggagaaa aaatcgatcc gtcgcgaaaa
     5521 aagagaatct tttgccgggg gcgccggggg aaggatggac ccgccggggc taggccccga
     5581 aggaggcctc ctatgcgggg gcgtcgtctt ccatcgggtt ttcgcggagc agcacctgtt
     5641 ttacgaaggc ctgggtcgcc cccgccaccg ggagggccag gagggctccc atcatccccg
     5701 ccagagaggt gcccaccagc atgacaagaa agaccagcag agggttcatg cggatgcttc
     5761 ggctctggac cacgggctgg agcacgttac tctcgatggc gccgtagatc agaaagaccc
     5821 ccaggagggc tgtggcgggc cacgatccca gggtggagaa ggtggtgctc accaccagca
     5881 ccgccgccag gacggggccc aggtagggca aaaggcccag gagggcggtg cagaacccca
     5941 gggcgagata gtaggggctt ccggccaaaa gaagggcggt gccgatgact cctcctccca
     6001 ccagggcgat gaagaccgtc ccggccacat aggcgctcac ggtggtctgc atggtgcgca
     6061 gggcggatcg gaccccggcc cggcgcctct cggcgacgaa ggccaacagg tcctcggtga
     6121 actcgttgcc gcagaggagc atgaacacgg tgaggaccaa caccatgccg agggaaagga
     6181 cccctgacag ggccccggtg accacgctca ccgccgcctg ggccaggtgg ggggcgttct
     6241 gagacgccca tccccggagc tgggcgaaga cgtggtactc ttcttccagg gcctggaccg
     6301 ccgccaggtt ctccagcttg gctatggcgt cgggggccac ccgcaccaag gcggcgatct
     6361 ggctcaccac cagggggatg atggtggaca ccgccacggc gatggcgctc cccgccagga
     6421 taaagaccac gccgatggcc catcgccggc ggagcccccg gcgctccagc ccgaggaccc
     6481 cggcattgag ggccaccgcc aggaaggccg atagcacgat ccaggtcatg acgtcccccg
     6541 tgcggtacac cacggcgatg atgccgccga tgagcaatag gtgcagggcc acggtccaga
     6601 tggtcttggg agatacctga gatgggggca tggcgaagtc ctcctcatgg gacaaggtaa
     6661 ggggtccggg gaaaggattt cttcgcccct tccagaagca ttatacggca tcggggcccg
     6721 ggccctttcg cctcgcggag ccccgccggg agataccccg gggccttaag gcgtggaggg
     6781 ggttggggta gaatgggagc cgtaaccctg cgtcgcctcg ggcgacgcga acgacggaaa
     6841 ccttaggcgg aggacctttc atggcgaaaa agaacgaaac acgctttgtc tgcacctcct
     6901 gtggggcccc ctcgtcgtcc tgggcgggga aatgcccccg gtgcggcgcc tggggatcgc
     6961 tggagcaaga tttcgccccc cggacggccc cgggaggggg caaagccccc gggggagttc
     7021 cccaagcccg ggccgtccgg gcgtcggagg tccccctcct cgaccgcatc cccacagggc
     7081 tggacgaact ggaccgggtg ctcggggggg gcttcgctcc ggggagcgtg tccctgctgg
     7141 cgggggagcc ggggatcggc aagtccaccc ttttgctcca agcctgcctg gccctggccc
     7201 gtcgaggaag gaccgtcctc tacgtctcgg gggaagaatc cttggagcag gtggcctctc
     7261 gggccctgag gctggacggt cagcccccgc ccctggacct tttggcctcc ggggatcccg
     7321 aggagttcat ggatctcctc ccgggacgat ccctggtggt ggtggacagc gtgcaggcca
     7381 tgggggacgg ggagtccctt cccggcaccc tgtcccaggt gcggggagtg gcggcccggt
     7441 gcatcgaggg ggccaaaaga agcggcgttc ccctggtgtt ggtgggacac gtcaccaagg
     7501 atgggcagat cgcggggccc aagctcctgg agcacatggt ggacgtggtg ctctccttcg
     7561 agggggatcg tcacacggcg taccggatct tgcgatcctc caagaaccgt ttcgggagca
     7621 ccgacgagat cggcgtcttc gagatggggg aggaagggct ccgccccgtg gcggacgcga
     7681 gcctactatt ctggaacggc gccgagggcg gggttcccgg cgtggctttc ggggtcacct
     7741 tggagggaag tcgccccttt gtggtggagc tccaggccct ggcctgtccc actcccttcc
     7801 cctatcccaa acgcaccgcc cggggagtgg aggtcaatag ggtgcaattg ctcctggcgg
     7861 tgctggaaaa acgttgcggc ctctccgccc gtaccgccga tgcctatgtg aacgtggcgg
     7921 gggggctggc cgtacgggat cccggggcgg acctggcgct ctgcgccgcc ctggcttcct
     7981 ccctgctcga tcgccccctg tcggcggggg ggtgtctggt gggagaagtg gggttggcgg
     8041 gggaggtgcg cccctgcccc cgcacggggg cccggttgca cgaggccgcc cggctcggtt
     8101 tcacccgagg tgtggtaagc tctttcgagg aaaagaactt cgacggagag ctggaggtgg
     8161 tgagggtggg gtcattgagc gaagccctgg cggccctctt tccccgatga gacgggaaaa
     8221 aagggggggc cccggagccc gaagatgggg actagggggg gttttgtgtc tagccctggc
     8281 gctgttatgg gcagggaaag cggcggcgga gagcctggtc atggtgacgc cccttcgggg
     8341 cgccgtggga gcggccctgg agcatcacct ggaagaagtg atccaggagt gcgataagga
     8401 gggcgtggcc cttctggtgc ttgagatcga taccccgggg ggactggtgt cctccatgcg
     8461 ggagatggtg aggatgatcc agaatgcccc ctgtcccgtg gtggcctggg tggcccccgg
     8521 gggggcccag gcggcttcgg cgggggcctt cctgttggag gcggcccacg tggcggccat
     8581 ggccgaaggc accaacgtgg gggcggctca tcccgtggga gtgggagggg gcgagggggg
     8641 ggagatggat cgcaagatca ccaacgacct gacggcccag atgcgatcct tggcccagct
     8701 tcgggggcga aacgccgatg cggcggcccg catggtgacg gaaagcgctt cctatccctc
     8761 ccgggaggcc ctggagggag gagtggtgga tctggtggtg ggggatctgg cggcactgat
     8821 ctcctccgtg gaggggcgta tcgtgaccat gccacaaaag gaggtggtcc tctccgtggg
     8881 ggcctaccgg gtgaaggctc ggcccatgtc cctcccccta aggatccttg aagtcatcac
     8941 tcgccccgac gtggcctatc ttctgttggt ggcggggatc tatgccctta tcttcgaggc
     9001 cctcaccccc ggcggctttg tcatgggaat ctccggggcg ctcctcgtgc tcctggggtc
     9061 tttgggaatg agaatgcttc ccttcagttg ggcggggatc cttctgctgg tggcgggagt
     9121 gggcgtcatg atcctggacc tggtggtggg aggcatgggt tttttgagcc tcttcggcgc
     9181 ggcggccctg gtggtgggag gattggtgat ctttcgggcc ccgggagggg agctcatgcg
     9241 cttttccatg gaagtcatgg ggggaggggt actggggatt tccctgtttt tcctgctctc
     9301 cgtctgggca gtgtggcggg cgatgcggca ccgggcggtt tcggggcagg aggggctggt
     9361 gggagcccgg ggtaggacct tgcaagagct gaacccccag gggatgatca agtgtcacgg
     9421 cgagtactgg aaggcccgta ccgaaagcgg ccttaagatc ccggacgaca ccccggtgga
     9481 agtggtggcc atggaggggt tgaccctggt ggtacgcccc cagagtcccg aaggggcgga
     9541 tggcgcgaaa agcgcgaagt aaagaaggag ggagttccca tggatctgtt aatggacgtt
     9601 ctctttagca ttttatcaaa cttcagtgcc ctgctgattt tggtgtttat gctggtatcg
     9661 gccattaaag tggtgccgga gtaccagcgc ctggtgctct ttcgcctggg gcgcctcgtg
     9721 ggatcccggg ggccggggct ggtcctcgtc attcccatgg tggaccggac cgcccgggtg
     9781 gacctccggg tggtcaccct ggacgtcccc gtgcaggagg tgatcaccca agataacgtc
     9841 cccatcaagg tcaacgcggt ggtctacttc cgggtcatgg atcccagccg atccgtggtg
     9901 gaggtggaga actacatcat ggccaccagc cagctttccc agaccaccct gcgatcggtc
     9961 attggccggg cggagctgga cgaggtcctg tcggatcggg acaagatcaa ccaggagctc
    10021 cagcagatca tcgacgagcg caccgacccc tggggcatca aggtgagcgc cgtggaggtc
    10081 aaggaactgg agcttccgga agggatgaag cgcgccatgg cgagccaggc ggaggccgaa
    10141 cgggaccggc gggccaagat catcaacgcc gaaggggagc tccaggcttc gcaaaagctc
    10201 ctggaagccg cccaggtcat ggagcgagcc cccatatcgc tccagctccg atacctccag
    10261 acgctgcgag agatcgccag cgagaaaaac tccaccacca tcttcccctt acccattgac
    10321 ctggtgaagc cctttttgga cgcctacctg aagccccgag gatgatggac ttaaaggggc
    10381 gaagggtccc cttgaagccc aagggcccgg ggaagggcct tcggcgggga aggcccttcg
    10441 agatgaagcg aaaaaagatc acgggaaaag agggatagca tgcgcatttt ggcgatcaac
    10501 gggagccctc ggggagctca agggaacacg gaagccatgt tgcagccctt tctggaagga
    10561 gcccgggctt tcggggcgga gaccgaagtg gtctacgccc gagacctctc ggtgggattt
    10621 tgtcaggggt gttttgcctg ctggttcaag actccggggc ggtgtatcct caaagacgac
    10681 atggaagccc ttctggagag attgcggcgg gccgatgcgg tgatttgggc cacgcccctg
    10741 ta
//
LOCUS       DAOOER010000058        10739 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_6647_length_10739_cov_8.780419, whole genome shotgun sequence.
ACCESSION   DAOOER010000058 DAOOER010000000
VERSION     DAOOER010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 10739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10739
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6647_length_10739_cov_8.780419"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..780
                     /locus_tag="PL162_07545"
     CDS             190..780
                     /locus_tag="PL162_07545"
                     /inference="COORDINATES: protein motif:HMM:NF020939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HQF91906.1"
                     /translation="MTLEAKKIISCVYRMAERSSGGAFPLPLLVAVLRGSRSRRVREL
                     GFQALSTWGLLKDTPPQRIQEVVAFLEARGLLASADRGLCFTAATRPFLRSDAPLFMV
                     PLAPDAPEDAPPKPDGKEILFEKLREMRRAIAQEEGVPPFVIFPDSTLREMCRRLPAT
                     SEALLAISGVGNVKLERYGERFLRVVNGWVTGSSEA"
     gene            940..1386
                     /locus_tag="PL162_07550"
     CDS             940..1386
                     /locus_tag="PL162_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009166157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQF91907.1"
                     /translation="MHATKGSVVKVHYRGTLRDGSVFDSSQGREPLEFVVGAGQMIAG
                     FDQGVEGMALGEERTLVIPPERAYGPWNEDLVMSVPRTNLPEGYDPAEGDQLHMALGP
                     RGRPFPVTVMAVEEDHVVLDGNHRLAGEELTFAVTLVALSGSPEVS"
     gene            1560..2045
                     /locus_tag="PL162_07555"
     CDS             1560..2045
                     /locus_tag="PL162_07555"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HQF91908.1"
                     /translation="MTSIVASSAAGTVGQAVTFTAEATATNCTLGSFTWNFGDGSPEE
                     VVRTSRSPSSALHAFAAPGTYEVRVTARSSTGENADSRTLAFSVAGISPTAVPTPGPS
                     ATPAPTGLPSPTSAPSPTGTPSGAPPSGGSGGGGCDGGLGGALPLLFLLPGLLAAGGR
                     P"
     gene            complement(2175..2417)
                     /locus_tag="PL162_07560"
     CDS             complement(2175..2417)
                     /locus_tag="PL162_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQF91909.1"
                     /translation="MKKIQILGTGCPKCKKLAEVAEAAARELGLEFELEKVTDIARIV
                     DFGVMATPALAVDGQVLSAGKALTKEDVMKLLSAVR"
     gene            complement(2671..3126)
                     /gene="arfB"
                     /locus_tag="PL162_07565"
     CDS             complement(2671..3126)
                     /gene="arfB"
                     /locus_tag="PL162_07565"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HQF91910.1"
                     /translation="MARPGEESPEDLREQISLELAGRALRVEFVRASGPGGQNVNKVA
                     TAVKLFFDLSASSLSPEERERFRRAAGQRISSAGVFLLDARRFRTQEANRRDALERLA
                     ELLRRATTPPAPRIPTRPGPGAQLRRRKLRDRLQARKKARRKDLRAEDD"
     gene            complement(3110..3892)
                     /gene="larB"
                     /locus_tag="PL162_07570"
     CDS             complement(3110..3892)
                     /gene="larB"
                     /locus_tag="PL162_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HQF91911.1"
                     /translation="MDEATLRVILRAVKSGALDENAALARLRLLPFEDLGDVRLDTHR
                     ALRTGFPEIIYAAGKSDEQLQRIARSLAPGEEDLLISRASEAQYDLVRREIPGVRYSP
                     EARLAYLRRRRGAPGGAVVVASGGSSDVPVAEEAALVAETLGCAVTRLYDVGVAGLHR
                     LLGSLETLRSAQALVVVAGMDGALPSVVAGLVSCPVFAVPTSVGYGAAFGGIAPLLTM
                     LNACAPGVSVVNIDNGLGGGYCAALAVRRRDPSGGEKSGKTR"
     gene            complement(3885..5042)
                     /gene="larC"
                     /locus_tag="PL162_07575"
     CDS             complement(3885..5042)
                     /gene="larC"
                     /locus_tag="PL162_07575"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HQF91912.1"
                     /translation="MIVLEPFCGVSGDMLLGVLCDLGGAPEILGRFEAFRSAGVRSVR
                     VSVERVLRRGIGATAVRVEVEEEEKERSWEELTTLLHEGARGVGASPAAVGRALKALE
                     LLGDAEARVHGHPREEVHFHETGSFDTVVDVLGFFLLLESLGNPALRSLAVRLGRGSV
                     RCAHGLYPVPPPAVAEIAARCHIPVEGGPVEGELATPTGMALLASAATFSPSLPSLIP
                     RRIGYGAGQRDLAVPNVLRGVWADDGEDEAVWMVEASLDDATGEEMGRALERLQEVSL
                     EAHLLSGVGKKGRPLFVLRVVAREEELSRVRDRLLEETPTLGVRSWPVARTRMAREVR
                     PLEVTVDGEPLPVRVKISRLGDVEKRKVEADDIARLRSRETDLRPREVPHG"
     gene            5300..6694
                     /gene="pepV"
                     /locus_tag="PL162_07580"
     CDS             5300..6694
                     /gene="pepV"
                     /locus_tag="PL162_07580"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF005591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HQF91913.1"
                     /translation="MTLDDHVAALQPQLISSLREALRLRSTPGTPLPGKPFGEGVDEA
                     LRHALKTAQDLGFRTANVDSMVGYAEYGEGEEMVAVLGHLDVVPEGTGWSHDPYGAEI
                     VDGVLYGRGTLDDKGPTYAALFALAAIRDGGIPLRRRIRVIFGTNEESGCACMAHYVQ
                     SGQELPVMGFTPDGAFPIINAEKGSVKLRLFSPGAPGGPENLTALTAAPAANMVPETA
                     EGTIEGPPERLLPLRRALESFAADREWPLVLEEGEGKLTVRPRGKAAHASLPELGINA
                     AVRLLRLLGALPTEAPWRRAAAALGALVNEKSDGAALGIACRDDLSGPLTLNLGLLEV
                     TADGGIAATLDIRYPVRQAWDALFPEVQARVRDLGLSLEVLRHSPPLFVDPSSELIQA
                     LQRAYTATTGQEAFLLAIGGGTYARTMPHVVAFGATFPGDPELMHQPDERIPLERLAL
                     ITRILAAAMKELAQ"
     gene            6849..7217
                     /locus_tag="PL162_07585"
     CDS             6849..7217
                     /locus_tag="PL162_07585"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HQF91914.1"
                     /translation="MIHNLKLRREALERAHRRCEKCGCLTNLKVYPVVPFAAGGADSL
                     DNVVVLCLYCHREMCQMKAGGFDFRAWLSLPPAPVVAKMVLESPEGVPVETLRDLLRT
                     LAEEARGDEAFWWGKNEGLF"
     gene            complement(7239..8033)
                     /locus_tag="PL162_07590"
     CDS             complement(7239..8033)
                     /locus_tag="PL162_07590"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HQF91915.1"
                     /translation="MISRRKAFSWCGVGGLLLVLGGVLPGRVIEAVPRQDAVVARYAK
                     DLPAAWGERLEGVRTRLATTDRVLALTLDACGGEYDEELVAFLIRERIPATLFLTGRW
                     IDRHPREAAALVQNPLFEIANHGENHRPASVTGRRAYGIAGTKSPREFAEELLISDAK
                     LRALGVPAPRYYRSGTNFYDDVALAMAAELGYEVVGYAVAGDGGATFSREQVKATVLR
                     SRPGDIILCHMNRPRGDTAEGLTEALPLMIREGFRFVTLSAYPLRD"
     gene            complement(8030..8488)
                     /locus_tag="PL162_07595"
     CDS             complement(8030..8488)
                     /locus_tag="PL162_07595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HQF91916.1"
                     /translation="MIIDHLSNAGFYFGADPLLREALEFLRLPEIATLGMGRHSLRGD
                     DLFALVSGGVTVAEGDAPWEAHRRYVDLHHVVEGGEWMGYAPLHRMRQTRSYDEGEDC
                     ALYRGEGNFLRVFSGMFVVFNVTDVHKPDIHLGEPEAFRKIVVKIRRREP"
     gene            complement(8531..9181)
                     /locus_tag="PL162_07600"
     CDS             complement(8531..9181)
                     /locus_tag="PL162_07600"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HQF91917.1"
                     /translation="MKVFSYPEGPRRRGGWRKGLGGAAILIMGALVAWYLLSPRGPGE
                     RPPTPLPPVELPTPTPSPPPLPTPFSQAERNPLKEGSLERAWIRIVKSAFILELYEGR
                     DLVRTYPIATGSALGDKRSQGDRRTPVGRFSISQIQPSSSWTHDFGDGKGAIKGAYGP
                     WFLRLKTGWKGIGIHGTHDPDSIGKSVSEGCIRMFNADVAELRRRVRPGTPVIIEP"
     gene            complement(9348..10586)
                     /locus_tag="PL162_07605"
     CDS             complement(9348..10586)
                     /locus_tag="PL162_07605"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HQF91918.1"
                     /translation="MRIWMIAQDTRPPEVPGITRHYALGREWVSRGDEVLVVHGSFDH
                     LGPSLPEGPPPEGVWDRSYDGVRFLTVPVPPYGGSATPGRLWNNWLFGRRVEKILGGG
                     SYGVPQVVLGSTVHPWAARAGLRLARRFRVPFVFEVRDLWPQTLVELGRIFPGHPLVR
                     YFGAVERTLAQSADLVVTSAPRMGEYFRERYSLPEERWCWITNGTFVEAFDPSPPEED
                     EGALEIFYTGTLGRANRLDRFFALLPDLARRFPTLRFVFVGRGEMRRELEERGRLQGL
                     PVRFEDPVPKRRVPELLRRASAFLVVLDDLPLYRYGTSLNKMADYHAAGRPVILLGDC
                     AENPLAASGAGWVAPREEDLPGALEALVRAGVEGRRELGRRGREYAREHYDWPKLAER
                     LRGRLEKVVSRRPPESSPRR"
BASE COUNT         1665 a         3849 c         3506 g         1719 t
ORIGIN      
        1 ttcggagacc gcccccccgc cgttccctgc ggagggtgcg gcccctgtct aggggccccc
       61 cggaaggtcc aaatcgtgtc ctttttccga tcccttcgcc cttcgatcct tccccatccc
      121 cctatatcgc tcacgcccgc cctccccccc acgccctccc tcccccccgc gcccgccccg
      181 ggggaggatg tgaccctgga ggcgaaaaag atcatctcct gcgtctatcg catggcggaa
      241 cgatcctccg gaggggcctt tccccttccc ctgctggtcg ccgtcctccg gggaagccgg
      301 agccgccggg tgcgggagct gggctttcag gccctctcca cctggggatt gctgaaagac
      361 acccctcccc agcggatcca agaggtggtg gccttcctgg aggcccgggg acttctggct
      421 tcggccgatc ggggcctgtg tttcaccgcc gccacgcgcc cctttctccg gagcgacgcc
      481 cccctcttca tggtcccgct ggctccggac gcccccgaag acgctccccc caagcccgat
      541 ggaaaggaga tcctctttga aaagctccgg gagatgcggc gagccatcgc ccaggaagag
      601 ggagtgcccc ccttcgtgat ctttccggac agcaccctga gggaaatgtg ccggcgtctc
      661 cccgccacct ccgaggccct tctggccatt tccggcgtgg ggaacgtcaa gctggaacgc
      721 tacggcgaaa ggtttctccg ggtggtcaac ggctgggtca cgggaagttc ggaggcgtga
      781 ccttctccgg cttcgcccct cctcgggcgc ttgtgccccc ctgctacgta cagtacaata
      841 tggtaccgat ccgaaagtct ttcggaatcc atgggttcca cgcccttcgc ggaaagggcg
      901 cacttcaggc catgccatgt cggccgaaag gagcgtgcca tgcacgccac caagggaagc
      961 gtcgtcaagg tacactatag gggaacgctg cgggacgggt cggtcttcga ttcctcccag
     1021 gggcgggagc ccctggagtt cgtggtgggg gcggggcaga tgatcgcggg cttcgaccag
     1081 ggcgtggagg ggatggccct gggggaggaa cgcaccctgg tgatcccccc ggaaagggcc
     1141 tatggcccct ggaacgagga cctcgtgatg tcggtccccc gcaccaacct ccccgagggg
     1201 tacgaccccg ccgaggggga ccagcttcac atggccctgg ggccccgggg gcgccccttt
     1261 ccggtcaccg tgatggccgt ggaggaagat cacgtggtgc tggacggcaa ccaccgcctg
     1321 gcgggggagg agctcacctt cgccgtgacc ctggtggccc tctcggggag ccccgaggta
     1381 tcctaggggc ttcgccgtct gcggggcccc cgtccccggg cggccccatc ttttgaagat
     1441 agggggcttt tgtcgtgaaa ataacgcatc ttcttcggat ttcggcgctc ggcctcaccc
     1501 tcctgggggt tccggagggg cttcccgcct tcgggtgagg atcggatgcc caggtccggg
     1561 tgacctccat cgtcgcgtct tctgctgcgg gcaccgtggg gcaggccgtg accttcaccg
     1621 ccgaagccac ggcgactaat tgcaccctgg gatcttttac ctggaacttc ggcgacggct
     1681 cgccggagga ggtggttcgg acctcccggt ccccctcatc cgccctccac gccttcgccg
     1741 cccccgggac ctacgaggtc cgcgtcaccg cccgaagcag caccggagag aatgcggact
     1801 cccgcacgct cgccttttcc gtggccggaa tctcccccac ggcggtgccc acgccgggac
     1861 cctccgccac tcccgccccc acggggcttc cgtcccccac ttccgcccca tctcccacgg
     1921 gaacaccttc gggcgctcct ccctcggggg gctccggagg cgggggctgc gacggggggt
     1981 taggcggcgc cctgccgctt ctgttcctgc tccccggcct tctggcggcg ggcggtcgac
     2041 cctagaccac caccgggaaa atgaaccaac gccggggccc ccttgaaggg gccccggcgc
     2101 ttttcgtttt ggggcaaaga cccggggatc ggccgtcccc ttccgggggg ctcatcgggt
     2161 cactccgggg tggctcaccg caccgccgac aaaagtttca tcacgtcctc tttcgtcagg
     2221 gccttgcccg ccgatagcac ttggccgtcc accgccagag cgggggtggc catgacgcca
     2281 aaatccacta tccgggcgat gtcggtgacc ttctccagct cgaactccag gcccaactcc
     2341 cgagcggcgg cctccgccac ctccgccagc tttttgcact tggggcatcc ggtcccaaga
     2401 atctgaattt ttttcatgct ttttctcctt ccgaccgatg gggtaagagc cttttgtgtg
     2461 gcaaaagcac cacataatcg aaagaaactc aagggcacac cccggcgtcg gcgatctccc
     2521 cggatctttg gacgtcgagg gggcgacccc aggggatcct cgccccgagc gatccgcgct
     2581 gcggagcaag ggcgcggtcc ttctcccccc cggggggctc ctccggcggg atcgggcgag
     2641 gggcgcccct cggctttcga gagaggggga tcagtcgtct tcggcgcgaa ggtctttccg
     2701 ccgggccttt ttccgggcct ggaggcggtc ccggagcttt cttcgccgga gctgggctcc
     2761 ggggccgggg cgagtgggaa tccggggagc cgggggggtg gtggcccgac ggaggagttc
     2821 cgccagccgc tccagggcgt cccgtcgatt ggcctcctgg gtgcgaaacc gccgggcatc
     2881 gagcagaaag acccccgcag aggagatccg ttgccccgcc gcacggcgga agcgttcccg
     2941 ctcctccggc gaaagggagg aggcggaaag gtcaaaaaat agtttgaccg ccgtggcgac
     3001 tttgttgacg ttctgccctc cgggtcccga cgcccgcacg aactccaccc ggagggcccg
     3061 tcccgcgagc tccaacgaga tctgttcccg gaggtcttcg gggctttctt caccgggtct
     3121 tgccactctt ctctcccccc gacggatccc ggcgacgcac cgccagggcg gcgcagtacc
     3181 ctccccccag gccgttgtcg atatttacca cggagactcc gggggcacag gcgttgagca
     3241 tggtcagcag gggggcgatg cctccgaagg cggcgccgta ccccacgctg gtggggacgg
     3301 caaagaccgg gcaggacacg agccccgcca ccacggaggg gagggcccca tccatccccg
     3361 ccaccaccac cagggcctgg gcgctgcgaa gggtctccag ggagcccagg aggcgatgga
     3421 gtcccgccac ccccacgtcg tagagccggg tgaccgcgca tcccagggtt tccgccacca
     3481 acgccgcctc ctccgccacg gggacgtcgc tgcttccgcc gctggccacc accaccgccc
     3541 ctccaggggc cccccggcga cggcgcaggt acgcgaggcg agcctcgggg gagtagcgca
     3601 ccccgggaat ctcgcgcctc accaggtcgt attgggcctc cgaggcccgg gagatgagga
     3661 ggtcttcctc ccccggggcc agggaccggg cgatgcgctg gagctgctcg tcgctttttc
     3721 ccgcagcgta gatgatctcg ggaaagcccg tgcgcagcgc ccgatgggtg tcgaggcgca
     3781 cgtcccccag gtcctcgaag ggcaggaggc gcaggcgggc cagggcagcg ttttcgtcca
     3841 gggctccgct cttcacggct cggaggatca cccgcagggt ggcttcatcc atgggggacc
     3901 tcccgggggc ggaggtccgt ctcccgggat ctcaggcggg cgatgtcgtc cgcttcgacc
     3961 tttctcttct ccacgtcccc gagacgggag atcttcaccc gcaccggcag gggctccccg
     4021 tccacggtga cctccagggg gcgcacctct cgggccatgc gggtccgggc cacgggccag
     4081 gagcgtaccc ccagggtggg ggtctcttcc agcaggcgat cccgcacccg ggagagttct
     4141 tcctcccggg ccaccacccg gaggacgaag aggggtctcc ccttctttcc cacccccgac
     4201 aggaggtggg cctccaggga gacctcctgg agtcgctcca gggctcgccc catctcctcc
     4261 cccgtggcgt cgtccaggct cgcttccacc atccacaccg cctcgtcttc tccgtcatcc
     4321 gcccagaccc cccggaggac gttgggcacg gccagatccc gctgcccggc gccgtagccg
     4381 atccgccggg ggatgaggga gggcaggctt ggggaaaagg tcgccgccga ggccaaaagg
     4441 gccatgccgg tgggggtggc gagctccccc tccacggggc ccccctccac ggggatgtga
     4501 caccgggcgg cgatctccgc cacggcgggg gggggcacgg gatagaggcc gtgggcgcac
     4561 cggacggagc cccgccccag gcggacggcg aggctgcgca gcgcggggtt tcccagggac
     4621 tccagcagga ggaaaaagcc cagcacgtcc accacggtgt cgaagctccc cgtctcgtga
     4681 aagtgcacct cctcccgggg gtggccgtgc acccgggcct cggcgtctcc caggagttcc
     4741 agggccttca acgcccgacc caccgccgcc ggagaggctc ccacgccccg ggctccttcg
     4801 tggagcagcg tcgtcagctc ctcccaggag cgttcctttt cttcttcttc cacctccacc
     4861 cgcacggcgg tggcgccgat gccccgccgc agaacccgct ccaccgacac ccgcacggag
     4921 cgcacgccgg cggaccggaa ggcctcaaaa cgccccagga tctccggcgc cccccccagg
     4981 tcgcagagca cgccgaggag catgtccccg ctgacgccgc aaaatggttc tagaacgatc
     5041 atggatccct ctcccttcca cctcttcatc cctacatctc ttcatcctta catctcttca
     5101 tctcatccct ggggttcagc ctcttcgggc tcacccccgg caccaattcc ggggcggcgc
     5161 cgggatcgag gggggtaagc cccccagatt ttcccctctc atggtacact gtttcggcca
     5221 tgggaaacaa cgcccgatcc cccccttttt taggtcaagg ggacggcgaa aactttttta
     5281 attcaggagg agcgcgatca tgaccctcga cgaccacgtg gcagcccttc agccccagtt
     5341 gatttcttcc ctgcgggagg cccttcgcct caggagcact ccggggactc ccctccccgg
     5401 caagcccttc ggcgaagggg tggacgaagc cctgcgtcac gccctgaaaa ccgcccagga
     5461 tctgggtttt cgcacggcca acgtggattc catggtggga tatgcggagt acggcgaagg
     5521 ggaggagatg gtggcggtac tgggccacct ggacgtggtc cccgagggga cggggtggag
     5581 ccacgacccc tacggggcgg agatcgtgga cggggtgctc tacggccgag gaacgctgga
     5641 cgacaagggg cccacctacg ccgccctctt cgccctggcg gccataaggg atggagggat
     5701 ccccctgagg cggcgtatcc gggtgatctt cggcaccaac gaggagtcgg gatgcgcctg
     5761 catggcccac tacgtccaga gcggccagga gctccccgtc atgggcttca cccccgacgg
     5821 ggcctttccc atcatcaacg cggaaaaagg aagcgtcaag cttcgcctct tctcccccgg
     5881 agcccccggc ggccccgaga atctcacggc cctgaccgcc gcccccgcag ccaacatggt
     5941 ccccgaaacg gcggagggca ccatcgaggg gcccccggag aggctccttc ccctgcgccg
     6001 ggccctggaa tccttcgccg cagaccgcga atggcccctg gtcctggagg aaggggaggg
     6061 aaagctcacg gtgcgccccc gggggaaggc ggcccacgca tctttgccgg agctcggcat
     6121 caacgccgct gtgcggctgc tccgtctgct cggggccctg cccacggagg ccccctggag
     6181 gcgggccgcc gccgccctcg gagccctggt aaacgagaaa agcgacgggg cggccctggg
     6241 gatcgcctgc cgggacgacc tgagcggccc cctcaccctg aacctggggc ttctggaggt
     6301 caccgccgac ggggggatcg ccgccacgct ggacatccga tacccggtgc gccaggcctg
     6361 ggacgccctc ttccccgagg tccaggcgcg ggtccgcgat ctggggctct ccctggaggt
     6421 cctgcgccac agccccccgc tcttcgtgga tccctcgtcg gagctcatcc aggccctcca
     6481 acgggcctac acggccacca cgggccagga ggcgttcctc ctggccatcg gaggagggac
     6541 gtacgcccgc accatgcccc acgtggtggc cttcggggcc acctttcccg gggatccgga
     6601 gctcatgcac caacccgacg agaggatccc cctggagcgc ctggcgttaa tcacccgcat
     6661 cctcgccgcc gccatgaaag agctggccca atagggcttc gcgtcgccgc cggggagagt
     6721 ttcggcctca ccgccggatc tttccccgaa gccggggctc gggcacgcca gggggcgaaa
     6781 aaaggcccct ccggggcgat atcccgggcc cccgtagagg gaacgcccaa aagaggaggc
     6841 gatctcccgt gatccacaac ctgaagctgc gtcgggaggc cctggaacgg gcccatcgtc
     6901 gctgcgagaa gtgcggttgc ctcacgaacc tcaaggtcta ccccgtggtg ccctttgccg
     6961 ccggaggggc ggattccctg gacaacgtgg tggtcctatg cctctactgc caccgggaga
     7021 tgtgccagat gaaggcgggg gggttcgact ttcgcgcctg gctctccctg ccccccgctc
     7081 cggtggtggc gaagatggtc ctggagtccc ccgagggcgt accggtggaa accctccggg
     7141 acctcttgcg caccctggcg gaggaggccc ggggggacga ggcgttctgg tggggcaaaa
     7201 acgaggggct attttagctc cgatgggtgg gggagggcct agtcccgcag ggggtaggca
     7261 gaaagggtca cgaagcgaaa gccctcccgg atcataaggg gcagggcctc ggtgaggcct
     7321 tcggcggtgt cgccccgggg gcggttcatg tggcacagga tgatgtcccc cgggcggctc
     7381 cggagcacgg tggctttcac ttgctcccgg gaaaaggtgg ctcccccatc tcccgccacg
     7441 gcgtacccca ccacttcgta ccccagttcg gcggccatcg ccagggccac gtcgtcgtaa
     7501 aaattcgtcc ccgaccggta gtatcggggc gcggggaccc ccagggcacg cagcttggcg
     7561 tcgctaatca ggagctcttc ggcgaactcc cggggacttt ttgttcccgc gatgccgtag
     7621 gcccttcgtc ccgtcaccga ggcgggacgg tggttctctc cgtggttggc gatctcgaac
     7681 agggggtttt ggaccagggc cgccgcctcc cgggggtggc ggtcgatcca ccgtcccgtc
     7741 aaaaagaggg tggcggggat tctctcccgg atgaggaacg ccacgagctc ttcgtcgtac
     7801 tcccccccgc aggcgtccag ggtcagagcc agcacccggt ccgtcgtggc cagccgggtc
     7861 ctcactcctt caaggcgctc cccccaggcg gcggggagat ccttcgcgta tcgcgccacc
     7921 acagcatcct gcctgggaac cgcctcgatg accctccccg gcaagacgcc ccccaggacc
     7981 aaaagcaacc cccctacccc gcaccatgaa aaggcctttc gccgggagat catggctccc
     8041 tccggcggat cttcaccacg atcttccgaa aggcctcggg ctccccgagg tggatgtcag
     8101 gcttgtggac gtcggtgacg ttaaagacca cgaacatccc cgaaaagacc cggaggaagt
     8161 tcccttctcc ccggtagagg gcgcagtctt ccccttcgtc gtaagaccgg gtctggcgca
     8221 tgcggtgaag aggggcgtac cccatccact ctcccccttc caccacgtgg tggaggtcga
     8281 cgtaccgccg atgggcctcc cagggggcat cgccttcggc gacggtgacg cctccggaga
     8341 ccagggcgaa gaggtcgtcc ccccggaggg aatggcgccc catccccagg gtggcgatct
     8401 ccggaaggcg gaggaactcc agggcctctc tcaacagggg gtccgcgcca aagtagaacc
     8461 cggcgttgct cagatggtcg atgatcacgg cgatcctctc ctttcagccc cccaatcccc
     8521 gacgaggggc ctagggttcg atgatcacgg gggtccccgg acgcacccgg cgccggagct
     8581 ccgccacgtc ggcattgaac atgcggatgc acccctcgga tacgcttttc cctatggaat
     8641 cggggtcgtg ggtgccatgg atccctatgc ccttccatcc ggtcttcagg cggagaaacc
     8701 acggtccgta ggcccccttg atggctccct tgccgtcgcc gaagtcgtgg gtccaggagg
     8761 aagagggctg gatctgggag atggaaaagc gccccaccgg ggtccgccgg tctccctggg
     8821 atcgcttgtc ccccaaggcg gacccggtgg cgatggggta ggtccgcacc agatctcgtc
     8881 cctcgtagag ttccaggatg aaggcgctct tgacgatgcg gatccaggcc cgctccaggg
     8941 agccctcttt caaggggttg cgctccgctt gggagaaggg ggtggggagg ggagggggag
     9001 agggggtcgg ggtggggagc tccaccggcg gcaggggcgt gggggggcgt tctccgggac
     9061 ctcgggggga caaaagatac caggcgacca gcgcccccat gatcaatatc gccgcccctc
     9121 cgagcccttt ccgccatccc ccgcgtctcc gggggccttc ggggtaggaa aaaaccttca
     9181 aagacccttc ctccttctgt gactcttttt ctgtgactct ttgatcttgt ttcccgtttt
     9241 actccggggc ctctttgccc tccccgcctc attccgggcc gcgatcggga cacctggacc
     9301 tcgacaactt ccgctgggcg aaaagggtcc gggcagggcc ccggcggtca tcgacggggc
     9361 gaggactccg gtgggcggcg ggacaccact ttttccaggc gtccccgtag cctctccgcc
     9421 agcttaggcc agtcgtagtg ctcccgggcg tactcccggc cccgacggcc cagctctcgg
     9481 cgcccctcga cccccgccct caccagggcc tccagagccc cgggcaaatc ttcctcccgg
     9541 ggggccaccc accccgcccc cgaggcggcc agggggtttt cggcgcaatc ccccaggagg
     9601 atcaccgggc gccccgccgc gtggtagtcc gccatcttgt tcaggctggt gccatatcgg
     9661 tagaggggga ggtcgtccag caccacgagg aaggccgagg cccggcgcag gagctcgggg
     9721 acccggcgct tcggcacggg gtcctcgaag cgcacgggga gcccctggag gcgccctcgt
     9781 tcttccagct cccgcctcat ctctccccgc cccacgaaga cgaaacgcag cgtcgggaag
     9841 cgccgggcca ggtcgggaag gagggcgaaa aagcggtcca ggcggttggc cctccccagg
     9901 gtgccggtgt aaaagatctc cagcgcccct tcgtcttcct ccggcgggga ggggtcgaag
     9961 gcctccacga aggtgccatt ggtgatccaa caccaccgct cctccggcag ggagtagcgc
    10021 tcccggaagt actcccccat gcggggggcg gaggtgacca ccaggtcggc gctttgcgcc
    10081 agagtccgtt ccaccgcgcc gaagtagcgc accagggggt gtccgggaaa gatgcgcccc
    10141 agctccacca gggtctgggg ccagagatcc cggacttcaa agacgaaggg cactcggaag
    10201 cgccgcgcca ggcgaaggcc cgcccgggcc gcccaggggt gcaccgtgga gcccagcacc
    10261 acctggggga cgccgtaact acctcccccg aggatctttt ccacccgacg gccaaagagc
    10321 cagttgttcc aaagtcttcc aggggtggcg ctccctccgt aagggggcac gggcaccgtg
    10381 aggaaacgca cgccgtcgta gctccggtcc cagaccccct ccgggggggg gccctccgga
    10441 agggagggtc ccagatggtc gaagctcccg tggaccacca gcacttcatc cccccgggag
    10501 acccactccc tccccagggc atagtgtcgg gttatgccgg ggacctccgg ggggcgggtg
    10561 tcctgggcga tcatccagat cctcacggcg atctccggaa gcccctcccg tcggggtgga
    10621 caaagccccc cgtcaccgag atccatccca acgccaccgt caccgccgcc gtcaccgcca
    10681 gggcaccgcc ggagggaagg gccgccccgt ctccgcctgc cccgccccgg tcaagatgg
//
LOCUS       DAOOER010000059        10692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_6678_length_10692_cov_8.542446, whole genome shotgun sequence.
ACCESSION   DAOOER010000059 DAOOER010000000
VERSION     DAOOER010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 10692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10692
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6678_length_10692_cov_8.542446"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="PL162_07610"
     CDS             <1..208
                     /locus_tag="PL162_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91919.1"
                     /translation="EGRREGIKKGVEKGRKEGVEKGRREGVEKGRKEGLRTSARNMLR
                     KGMPMALVAEVTGLSPDEVAALMD"
     gene            413..643
                     /locus_tag="PL162_07615"
     CDS             413..643
                     /locus_tag="PL162_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91920.1"
                     /translation="MWWVPVLYTAGVFLAALSLGMAIAFRVARKKGSISSTADVLRLL
                     GSGALSRIPRKKSPGEEPPPSVPPPPEKKAPR"
     gene            complement(657..1643)
                     /locus_tag="PL162_07620"
     CDS             complement(657..1643)
                     /locus_tag="PL162_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HQF91921.1"
                     /translation="MLLLRRDDIRAVFSMRDAIEADKEAFVAQTRGETRVPLRGAVDV
                     PGGQSLFMPAYVGGAVDRCGIKIVTVMPHNGARGKPVVPATVALLDRETGEVEALLDG
                     TTLTQMRTAAVSGVATELLAREDARVGALFGTGGQAECQLEALLAVRSLEEVRIFDPL
                     PGRAEAFATRCAPRGERSGARLIPAATPADAVRGADVITTVTTSATPVFRGDDVAPGV
                     HVNAVGSYTPDRRELDSTLMERAGRVFVDHREAVLAEAGDLLIPLAEGRFSLDRIVGD
                     LGELLEGRVVGRTSPEEITVMKTVGFATLDVVAAARVLEKARAAGRGEEVAW"
     gene            complement(1844..2692)
                     /locus_tag="PL162_07625"
     CDS             complement(1844..2692)
                     /locus_tag="PL162_07625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF037026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQF91922.1"
                     /translation="MAECSWEIRPMTRREVDWAVDLAAAEGWNPGLGDAEAFFAQDPG
                     GFLVGLADGEAVGCLSAVSYGPRFGFLGLYIVLPSFRGQGYGVQLWEQGMARLSGRVV
                     GLDAVVAQQENYRKSGFVYAHAQFRFEGVTGGGGAAPAIFPLSSVPFETLCRYDATLF
                     PAERPAFLAPWTRSPHSVGRAWLEDGELRGYGVLRKCRRGYKVGPLFAETPSGAEMLL
                     DELLGSVPPGEPFFLDVPEPNDAALALVRRRGMEAVFETGRMYVGKAPAVRLEGIFGV
                     TSFELG"
     gene            complement(2857..3789)
                     /locus_tag="PL162_07630"
     CDS             complement(2857..3789)
                     /locus_tag="PL162_07630"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HQF91923.1"
                     /translation="MDKGARDWLRRALRGALFIFIGVLWGGISWGAELPVLYVALIPQ
                     KEVAESLVGGRFRVKALLPPGADPHTFEPKPHQMVDLARARAYISAGLPFEGALVPRL
                     RGAAPELVVVAGDGGIDKIALTPSEELFAPDSGDTPRGHSRPHGEDGGGDPHFWTSPR
                     RMAVMAHTMAEALEALDPAGAEEYQKNLGTLEGAIAALDEELRALLAGCRGMAFFVFH
                     PAWGYLAADYGLRQIPIEVEGKEPKAADLARLVLWGRERGVRVIFVSPQFSRKGAAAV
                     AAEIGATLVDLDPLAPGWMENLRRAGHLLRDAVK"
     gene            4066..4938
                     /gene="dapF"
                     /locus_tag="PL162_07635"
     CDS             4066..4938
                     /gene="dapF"
                     /locus_tag="PL162_07635"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HQF91924.1"
                     /translation="MLFFTKMQAHGNDFVVIENACGKFSSEDLRALAVDVCTYKFSLG
                     ADGLLVLDPPREPATEFFMRIFNADGSEGEMCGNGARCLASFAHHHGFADAHMVFGSL
                     SGPVEATVTPPSVRLHLGTLSLADAKWDVPYTFHRQEFHYSFLTAGVPHCVIPLDSME
                     DFPPQNCLSLGRALRYDQERFPQGTNVNFMEVLGPHTLRIRTYERGVEAVTHSCGTGS
                     VASALTGVKRFGLQSPVEVHTPGGINVVEVEPEGTGELYRIHLQGEAHFVARGSLLDD
                     GLAALHQRRELAPA"
     gene            5136..6521
                     /locus_tag="PL162_07640"
     CDS             5136..6521
                     /locus_tag="PL162_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase 1"
                     /protein_id="HQF91925.1"
                     /translation="MEETKKTAPEGGRAWRRYAEDPRESALSRDLGEFLSSCKTEREA
                     ARFIEAALKSAGSLPVGHNKGFCPGDMVHLVWKNRAVAAARIGTAPLAEGFRLVGAHV
                     DAPRVDVKPHPLYEDCGLAMVDGQYYGGVKKYQWTNVPLALHGEFHLAGGVSKAVVVG
                     EAPEDPVLIIPDLAPHLDWETDKRTAKETVLGENLDAIGASRPDPGTEKEPALAALKR
                     HLKDVWGVEEEDLMAADLALVPAGPSRDVGFDRGILGAYGIDDRACSFLAFRAFLDLP
                     TPERTAVFLAVDKEEIGSEGVASAQGALLETFLLELLEGTGSDASPRGMRRCFARSEA
                     LSADVTEAANPLYKDFFDPRQLPVAGNGVTIMKYTGRGGKNVASEAPGEFVARVLERL
                     RADRVPWQPGSLGKVDKGGGGTIAMYLARLGMDVVDVGPAVLSLHSPCEMISKADVLS
                     TFDAYGAFWRH"
     gene            6712..8325
                     /locus_tag="PL162_07645"
     CDS             6712..8325
                     /locus_tag="PL162_07645"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQF91926.1"
                     /translation="MRKTSPSGRGTPQDLLLRRLPGAPPPGEEAVALRHNVFVPLPPG
                     SPAPPRGATPRCPQEGVSFREAVEGMCLYALELDAQGGVVFCNAFLLHVAGWDLGSVR
                     GKNWFDDLRSPDNPMPGERFRLGLRRGFIPPHHEATFLSHWGDRRRVRWHNTVLRDDE
                     GRVTGILSLGEDLTEQRRAEWEERKARIQAEALNKVVRAVGAHQDMDELLCRVDQQLN
                     RFMEIPCLMVALGHPEWRVVYRRGGQNRSPEPFPGEWAFFDRVLREGKPLHLPDGRGG
                     GALGPHLEGLLKIPEVHSIMAAPLVFGDRTLGVMMALSTDSDLVYSSDDMAFFAAAAS
                     PVALALENSRLFTEMGNLAMTDALTGVANRRSLFMLGEHEISRAVRRRSHISFFMLDL
                     DHFKNVNDRHGHRVGDGVLHHVAQICRRSLREEDLLGRYGGEEFVVVLPDTGHYEAWS
                     VAERLRGAVEEAPFHLEGRPLPITISVGVSTLPGGSPRIPLEALLDRADQALYEAKRA
                     GRNTVAGATFPDHFVDAARLSSLRRRQST"
     gene            complement(8271..9434)
                     /locus_tag="PL162_07650"
     CDS             complement(8271..9434)
                     /locus_tag="PL162_07650"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HQF91927.1"
                     /translation="MWGRNWGKGLALVGWVLLWGIPGEGAPVRLSAVGDLMVHDPQIG
                     AAWNETLQSYDFSPQISPLVPLLASADLTVGNLETTFGGPRRKYTGYPCFSTPDPWAT
                     ALKDAGFDGLFTANNHCLDRGREGVLRTLEVLAAEGLGSTGTFASSDRPPLILERGGL
                     TFAFLAYTYGTNGIPVPKDAPWMVNLLGQAISADLARCASADVRVVSLHFGNEYQLTP
                     SPAQRAAAKAALDGGARIVLGTHPHVLQEVEIFSPDVPGDARVVAWSLGNFISSQRTE
                     PRERSAVLHVDLERRGDEVALLGVSATPVWTRYRRLPGGKRDIQVVPAVEMLRRWDQG
                     ERGNWGKEEIRRLRGAERGVARALFGKRTPLSEDGVYVLWRLRREESLAASTK"
     gene            complement(9481..10521)
                     /locus_tag="PL162_07655"
     CDS             complement(9481..10521)
                     /locus_tag="PL162_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91928.1"
                     /translation="MDGVGPPDRSVWMMKRFFPALGACALVGGLLGVLRAETLAFPGL
                     QGMVAGSLLGYALGWNARGDAPVCWPFRARLALVLCAIPTYLAFHLGAFSFALARGAD
                     SPFFWLEEIAGGFHKEAFFSLGMTGPLYRTFHGVMTGGGWIFFNLLDGIFFGVFLLIL
                     LGVGLGQRFERPAEGAETEMASPLSCPFLTVMACAYLVLGSAGLWKLWHAVEVGPPPS
                     FNPASWRALERYAGPWRLVEEVPAIFRASGDRLFFLRVAGFEDLTGQSASGRFSFSVT
                     RRGSTYQGLLYTGRRDGKTWHPSGGAYPLRLRLAPDASFLEGAVTLFTVFGRRDGRFR
                     GYPRSEDPGGGE"
BASE COUNT         1871 a         3737 c         3369 g         1715 t
ORIGIN      
        1 ggaaggtcgc agagagggca taaagaaagg cgtcgagaaa ggtcgtaaag agggcgtcga
       61 gaaaggtcgt agagagggcg tcgagaaagg tcgtaaagag ggcctccgca cctcggcgcg
      121 gaacatgctc cgaaagggca tgccgatggc gctggtggcc gaagtgacgg gactttcccc
      181 ggacgaggtg gcggcgctga tggattgagg tgttttttgc aagacgccgg taatcatccg
      241 tagcccacat tctgcagaaa gcgggctgga agatgcgcgc tcccagcaaa agaaagcgaa
      301 cgtcgagaaa aagcctggtg gatcggatcc accgccgatg tcttcgggac ggacggctgg
      361 atctctggat tcaggaagcc ctgcggcgcc gttagagggg gggtgaaacg tcgtgtggtg
      421 ggtgccggtg ctctacactg cgggggtttt tctggcggcc ctatccttgg gcatggccat
      481 cgcctttcgc gtggcccgca agaagggctc gatctcttcc accgccgacg tgctacgcct
      541 tctgggctcg ggagctctgt cccggatccc tcggaagaaa tctccggggg aggaaccgcc
      601 tccatcggtc cctccccccc ccgaaaaaaa agccccccgg tgagcgcgtg gggcccctac
      661 cacgccacct cttccccccg ccccgccgcg cgagcctttt ccagcacccg ggccgccgcc
      721 accacgtcca gcgtggcaaa gcccacggtc ttcatcacgg tgatctcttc cggcgatgtc
      781 ctcccgacca cccgtccctc caaaagctcc cccaggtccc ccacgatgcg gtccagagaa
      841 aaacgcccct ccgccagggg aatcagtagg tcccccgcct cggcgaggac cgcctcccga
      901 tggtccacga agacccttcc ggcccgctcc atcaatgtgg agtccagctc ccttcggtcc
      961 ggggtgtagg atcccacggc gttcacgtgg accccggggg ccacgtcatc tccccgaaag
     1021 accggtgtgg ccgaggtggt cacggtggtg atgacatcgg ctccccgcac ggcgtccgcc
     1081 ggggtggccg cagggatcaa ccgggctccc gagcgttctc cccggggggc gcatcgggtc
     1141 gcaaaggcct cggcgcgccc aggcaggggg tcgaagatcc gcacctcttc cagagatcgc
     1201 acggcgagca acgcctccag ctggcattcc gcctgccccc ccgtgccgaa gagggccccc
     1261 acccgggcgt cttcccgggc caggagctcc gtggccaccc cggacacggc ggcggtgcgc
     1321 atctgggtga gggtggtgcc gtcgagcaac gcctccacct cccccgtttc ccggtcgagg
     1381 agggccacgg tggcggggac cacgggctta ccccgggccc cgttatgggg catcacggtc
     1441 acgatcttga tgccgcaccg atccacggcg ccccccacgt aggccggcat gaagaggctc
     1501 tgtcctccgg gcacatccac cgctccccgg aggggcaccc gggtctctcc tcgggtttgg
     1561 gccacgaagg cctctttgtc ggcctcaatg gcgtctcgca tggaaaacac cgcccgaata
     1621 tcatctcgac gcaaaagcag catggcatgc atctcctttc atcccggggc aaagccccgc
     1681 cttgatccgc cgatcgcttt tgtggagagg aacggagggc gccccggctc tccacgccac
     1741 cgaccgaaac gccggcaccg ggatcccccg gcgccgacag ccatgaccac cccccgtttt
     1801 ccctctcgat tccggcggat tggaccgagc gccccgcctc ttgtcagccc aattcaaagg
     1861 acgtgacgcc gaaaatcccc tccaaacgca ccgccggagc cttgccgacg tacatgcgcc
     1921 ccgtctcgaa gaccgcttcc atgccccggc ggcgcaccag agccaaagcg gcgtcgttgg
     1981 gctccggaac atcgaggaag aagggctccc ccggaggaac gctgcccaga agctcatcga
     2041 gaagcatttc cgccccggag ggggtctccg cgaagagggg gcccacttta tatccccggc
     2101 gacacttccg caacacccca tacccccgga gctctccatc ctccagccac gctcgcccca
     2161 cgctgtgggg cgagcgcgtc cagggggcca aaaacgccgg acgctccgcc gggaagagcg
     2221 tggcgtcgta gcggcagagg gtttcaaagg gaacggagga gagggggaaa atcgccggcg
     2281 cggcccctcc tccaccggtg accccctcga aacggaactg ggcgtgggca tagacaaatc
     2341 cggatttccg gtagttttcc tgctgcgcca ccaccgcatc caaccccacc acccgccccg
     2401 acagacgcgc catcccctgt tcccagagct gcacaccgta tccctggccc cgaaaagacg
     2461 ggagcacgat gtacaacccc agaaagccga aacgggggcc ataggacacc gccgaaaggc
     2521 accccacggc ctcgccgtcc gccaaaccca ccagaaagcc tccggggtcc tgggcaaaaa
     2581 atgcctccgc atcccccaga ccgggattcc atccctccgc cgccgccaga tccaccgccc
     2641 aatccacctc ccggcgggtc atggggcgta tttcccacga acactcggcc atgctctccc
     2701 tccttccgaa cttccgtctc gccgccctcc ggggaggaaa cgtctttcct ccatgatgcc
     2761 gcccaattcg gaggtctcgg ggcgagcata tttttctccc ctacgccgcc acatctgcaa
     2821 aagagatgat cccgcccggc gaaagatttt gctcccctac ttcaccgcat cgcgcaggag
     2881 gtgccccgcc cggcggaggt tttccatcca ccccggggcc aggggatcca gatccaccag
     2941 cgtagcacca atttccgccg ccacggcggc ggcgcctttg cgggaaaatt ggggagagac
     3001 gaagatcacc cgcactcccc tttctcgccc ccagagcacc aggcgggcca aatccgccgc
     3061 cttgggctcc ttaccttcca cctcgatggg gatctggcgg agaccgtagt ccgccgccaa
     3121 gtacccccag gcgggatgaa agacgaaaaa ggccatcccc cggcaccccg cgaggagggc
     3181 ccggagctct tcatccaggg cggcgatggc cccctccaga gtccccaggt ttttttggta
     3241 ctcctcggct ccggcgggat ccagcgcctc cagggcctcg gccatggtgt gggccatgac
     3301 ggccatgcgc ctcggcgagg tccaaaagtg gggatcgccc cccccatctt cgccgtgggg
     3361 ccgggagtgt ccccgggggg tgtcgccgga gtccggggca aagagctcct ccgaaggggt
     3421 cagggcgatc ttgtcgatgc ccccatctcc tgccaccacc accagctccg gagcggcccc
     3481 gcgcagccgg ggcaccaggg ctccctcaaa gggaaggccc gccgagatgt aggcccgggc
     3541 ccgggccaga tccaccatct gatggggctt gggctcgaag gtgtggggat cggccccagg
     3601 gggcaagagg gccttcactc ggaagcgccc ccccacgagg ctttcggcca cttctttttg
     3661 aggaatcaag gccacgtaca agacggggag ctctgccccc caggagatcc ccccccaaag
     3721 tactccgata aaaataaaaa gcgcccctct cagcgcccgc cgtaaccaat ccctagcgcc
     3781 cttgtccacg ccccctcccc ctttccgagg cccttttctc gcgtcttttg gcccccttcc
     3841 cccccgggcc cgattcccgg ggggcgcttt ttagcatagc gggagctccc tccctgggca
     3901 acttattttt tctcactccc cgccgatgat atgggtaaaa cctctgatcc ttcggagtgt
     3961 ggcggggaga agttgtggta tattcttccc cagggagggg cgcccctcaa ggggcccccg
     4021 gggaagggaa aatcgaaatt atcttttcga aagagggtga cgggcatgct tttttttacg
     4081 aagatgcagg cccacgggaa tgacttcgtt gtcattgaaa acgcctgcgg aaagttttct
     4141 tcggaggatt tgagggccct ggcggtggac gtatgcacct ataagttctc cctgggggcc
     4201 gatggcctcc tggtgctgga ccccccgagg gaaccggcca cggagttttt catgcgcatc
     4261 ttcaacgccg atggctccga aggggagatg tgcggcaatg gggcccgatg tctagcgtcc
     4321 tttgcccacc accacggctt cgccgacgcc cacatggtct tcggatccct gagcggcccg
     4381 gtggaggcca cggtgactcc cccttcggtg cggctccacc tggggaccct ctccctggcg
     4441 gacgccaagt gggacgtgcc ctacaccttc catcgccagg agtttcacta cagctttctc
     4501 accgcagggg tcccccactg cgtgatcccc ctggactcca tggaggactt tccccctcag
     4561 aactgcctat ccctggggcg ggccctgcgc tacgaccagg agcgctttcc ccaaggaacc
     4621 aacgtcaact tcatggaagt cttaggccct cacaccctgc gcattcggac ctacgagcga
     4681 ggagtggagg ccgtcaccca ctcctgcggc acgggctccg tcgccagcgc cctgacgggg
     4741 gtgaagcgct ttggcctcca atctcccgtg gaggtccaca ccccgggggg gatcaacgtg
     4801 gtggaggtcg aacccgaggg aacgggggag ctctaccgga tccacctcca gggggaggcc
     4861 cacttcgtgg cccggggatc actcttggac gacggtctgg cggccctgca ccagcgacgg
     4921 gagctggccc cggcctagcc ccggggccct caggagctct ccggcaccat ccccccccga
     4981 ccctgccttc cgccttccct attttttgtg gtgtttttgt ggtggtaagg tagcgggggg
     5041 gtttttgtgc tatcatcact cccaagattt cgccggatgg gcccaagtcc cggggctttt
     5101 cggcgaagcg caccacataa ggaggcatca tttccgtgga agagaccaaa aaaaccgccc
     5161 ccgaaggggg tcgggcctgg cgtcgctacg ccgaagaccc ccgggagagc gccctctccc
     5221 gggatctggg ggagttcctc tcctcctgta agaccgaaag agaagccgcc cggttcatcg
     5281 aggccgctct gaagtccgcc ggaagccttc cggtgggcca taataagggc ttttgccccg
     5341 gagatatggt ccatctggtg tggaaaaacc gcgccgtggc ggcggcccgg atcggcacgg
     5401 cccccctggc cgagggcttt cgcctggtgg gggcccacgt ggacgccccc cgggtggacg
     5461 tgaagcccca ccccctctac gaggactgcg gtctcgccat ggtcgacggt caatactacg
     5521 gcggcgtcaa gaagtaccaa tggaccaacg tgcctctggc gctccacggg gagtttcacc
     5581 tcgccggagg ggtctccaag gcggtggtgg tgggggaggc tccagaagac cccgtgctga
     5641 tcatccccga cctggcgccc catctggact gggagacgga caagcgcacc gccaaagaga
     5701 ccgtcctggg agagaacctg gacgccatcg gcgcctcccg tcccgacccg gggaccgaaa
     5761 aagagcccgc cctggcggcg ctgaaacgcc acctgaaaga cgtctgggga gtggaggaag
     5821 aggatctcat ggccgccgac ctcgccctgg tgcctgcggg gccctcccgg gacgtgggct
     5881 ttgaccgggg aattctggga gcctacggca tcgacgaccg ggcctgttcc ttcctggcct
     5941 tccgcgcctt tctcgacctc cccacgccgg aacgcacggc ggtctttctc gccgtggaca
     6001 aagaggaaat cggcagcgaa ggcgtggctt cggcccaggg ggcgctcttg gaaacctttc
     6061 tgctggaact cttggagggt acgggaagcg acgcttcgcc gcgggggatg cggcgctgct
     6121 tcgcccgctc cgaggccctc tccgccgacg tcaccgaggc cgccaacccg ctgtacaagg
     6181 actttttcga tccgcgtcag cttccggtgg cgggaaatgg ggtcaccatc atgaagtata
     6241 ccgggcgcgg tggcaaaaac gtcgccagcg aggccccggg ggagttcgtg gcccgggtgt
     6301 tggagcgcct ccgggccgac cgggtgccct ggcagccggg gagcctgggc aaggtggata
     6361 aggggggcgg ggggaccatc gccatgtacc tggcccgcct gggcatggac gtggtggacg
     6421 tggggcctgc ggtgctgtcg ctccactccc cctgcgagat gatcagcaag gcggacgtcc
     6481 tttccacctt cgacgcctac ggagcctttt ggaggcactg atttcctctt tacggatcgc
     6541 tggcgccccc ccagggagat gccctggggg gggcctcccg aagcgatccg cagcgcacga
     6601 gggagtatcc ccaaaaaggt cccttgggcg cgaatcccgc cccccttcgt ttcgccgggg
     6661 gcggcttttg tcggctcccg tgaccctgga gaccctagga ggtgccgtcg gttgagaaaa
     6721 acttccccgt ccggtcgagg aacgccccaa gatctccttc tccgacgcct tcccggggcc
     6781 ccgcctcccg gagaagaggc cgtggccctt cgccataacg tcttcgttcc cctcccgccg
     6841 gggtcgcccg cgccccccag gggggcgacc ccgcgctgtc cccaggaggg ggtttccttt
     6901 cgggaggcgg tggagggcat gtgcctctac gccctggagc tcgatgcgca gggcggggtg
     6961 gtcttttgca acgccttcct gcttcacgtg gccgggtggg acctgggatc ggtgaggggg
     7021 aaaaactggt tcgacgacct gcgttccccc gacaatccca tgccgggaga acgcttccgc
     7081 ctggggttac gccgggggtt catccccccc caccacgagg cgacctttct cagccactgg
     7141 ggggaccgac gtcgggtccg gtggcacaac accgtgttgc gggacgacga ggggcgggtc
     7201 accggcatcc tgagcctggg ggaggacctc accgaacagc gtcgggcgga gtgggaggag
     7261 cgcaaggccc gcatccaggc cgaggccctg aacaaggtgg tgcgggccgt gggggcccac
     7321 caggacatgg acgagctgct ctgtcgggtg gatcagcagc taaaccgctt catggagatc
     7381 ccctgcctga tggtggccct cgggcacccg gagtggcggg tggtctaccg gaggggcggg
     7441 caaaaccgaa gcccggaacc cttccccggg gagtgggcct ttttcgaccg ggtgctccgg
     7501 gagggaaagc ccctgcacct tcccgacggc cgggggggcg gggccctcgg cccccatctc
     7561 gaagggctct taaagatccc cgaggtccat agcatcatgg cggcccccct cgtcttcggg
     7621 gatcggaccc tgggggtcat gatggcccta tctacggact ccgatctcgt atactcgtcg
     7681 gacgacatgg ccttcttcgc cgctgcggcg tcccccgtgg ccctggccct ggagaactcc
     7741 cgcctcttca ccgagatggg aaacttggcc atgaccgatg ccctcacggg agtcgccaac
     7801 cggaggtccc tcttcatgtt gggagagcac gagatttccc gggcggtgcg gcgacgctct
     7861 cacatttcgt tcttcatgct cgacctggat cacttcaaaa acgtcaacga ccgccacggc
     7921 caccgggtgg gggatggggt actacaccac gtggcccaga tctgccgccg atccctccgg
     7981 gaggaggacc tcctggggcg atacggcggc gaggagttcg tcgtggtgct gccggatacg
     8041 gggcactacg aggcctggag cgtcgcggag aggctgcgcg gcgccgtgga ggaggccccc
     8101 ttccacctgg aggggcgtcc cctccccatc accatcagcg tgggagtttc cacgctcccc
     8161 gggggttccc ctcgtatccc cctggaggcc ctcctggacc gggcggatca ggccctctac
     8221 gaggccaagc gcgccgggcg caacaccgtg gcgggagcca cctttcccga tcacttcgtc
     8281 gacgcggcga ggctttcctc cctccggaga cgccagagca cgtagacccc gtcctcggac
     8341 aggggggtcc tcttgccaaa aagggcccgg gccacgccgc gctccgcgcc ccggaggcgg
     8401 cgaatttctt ccttgcccca gttcccccgc tccccttgat cccaccgacg gagcatttcc
     8461 accgcaggca ccacctggat atctcgcttc ccacccggga ggcggcggta ccgggtccac
     8521 accggggtgg ccgagacccc caaaagcgcc acctcgtccc cccggcgctc caggtccacg
     8581 tggagcacgg cgctccgctc ccggggttcg gtgcgctggg aagagatgaa gtttcccaaa
     8641 gaccaagcca ccacccgggc atccccgggg acgtcggggg agaagatctc cacctcctgg
     8701 aggacgtggg ggtgggtccc cagcacgatc cgggcccctc catccagggc ggccttggcg
     8761 gcggcgcgct gcgccgggct gggggtgagc tggtactcgt tgccaaagtg gagagagacc
     8821 acccgcacgt ccgccgaggc gcaccgggcc aggtcggcgc tgatggcctg ccccaggagg
     8881 tttaccatcc agggggcgtc tttgggcacg ggaatgccgt tggtaccata ggtgtaggcg
     8941 aggaaggcaa aggtgagccc cccccgctcc aggatcaggg gggggcgatc cgaggaggca
     9001 aaggtccccg tagaccccag cccctccgcc gccaggacct ccagggtgcg caagaccccc
     9061 tcccgccccc ggtcgaggca gtggttattg gcggtgaaga gcccgtcgaa gcccgcatct
     9121 ttcagggccg tcgcccaggg atcgggggtg gaaaagcagg ggtatcccgt gtacttccgg
     9181 cgggggcccc cgaaggtggt ctccaggtta cccaccgtga ggtcggcgct ggccaggagg
     9241 gggaccaggg gagaaatctg gggggaaaag tcgtaggact gaagggtctc gttccaagcg
     9301 gcgccgatct gggggtcgtg caccatgagg tctcctacgg cggagaggcg caccggggcc
     9361 ccctccccgg ggattcccca caagagcacc catcccacca gcgccagccc ctttccccag
     9421 ttccgtcccc acaccgtcgc cgcccccctt ctccttcttc gcctctttgg tccttctctt
     9481 ttactctccc cccccgggat cttccgaccg ggggtacccc cgaaaacgtc cgtcccggcg
     9541 cccgaacacg gtaaaaaggg tcaccgcccc ctccaaaaaa gaggcgtccg gagccagccg
     9601 caggcgaagg ggatacgctc cccccgaggg gtgccacgtt ttgccatcac gcctccccgt
     9661 gtacaggagc ccctggtagg tgctcccgcg ccgggtgacc gaaaaggaaa aacgccccga
     9721 ggcgctttgg cccgtcaggt cttcgaaccc cgccacccgc aaaaaaaata gtcggtctcc
     9781 ggaggcgcga aaaatcgccg ggacttcctc caccaggcgc caggggcccg cgtagcgctc
     9841 cagggcccgc cacgaagcgg ggttaaaaga agggggaggc cccacctcca ccgcgtgcca
     9901 cagcttccag agtcccgcac tccccaagac aagataggcg cacgccatga ctgtcaggaa
     9961 ggggcaggaa agcggagacg ccatctccgt ctcggcgccc tcggcagggc gctcgaagcg
    10021 ctgtcccagc cccaccccca ggagaatcag caagaagacg ccaaaaaaga ttccatccag
    10081 caggttgaaa aaaatccacc ccccgccggt catcacccca tgaaaggtcc ggtagagagg
    10141 gcccgtcatg cccagggaaa agaaggcctc tttatgaaac ccccccgcga tctcctccag
    10201 ccagaagaag ggggaatcgg ctccccgggc cagggcgaag gaaaaggcgc cgaggtggaa
    10261 ggcgaggtag gtggggatgg cacagaggac cagcgccaac cgcgcccgaa agggccagca
    10321 taccggagcg tccccccggg cgttccagcc cagggcatac cccaaaagtg atcccgccac
    10381 catgccctga aggccgggga aggccagagt ttccgcccgg agcaccccca aaagcccccc
    10441 caccagggcg cacgctccca gggcggggaa aaagcgcttc atcatccaga cgctccggtc
    10501 cggaggcccc actccgtcca tgtcccttcc cccttctcac gccccttacc cagggcgttt
    10561 cgtcaccagc ggtcctctcc tggggcggtc agaacttctt ctcctgggtg gccacctcta
    10621 ctccgggaat atactctttc aagagatggc gcaggggcca ttcgtcctcg cgcctaaagt
    10681 agagccgcaa cc
//
LOCUS       DAOOER010000060        10613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_6735_length_10613_cov_8.437962, whole genome shotgun sequence.
ACCESSION   DAOOER010000060 DAOOER010000000
VERSION     DAOOER010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 10613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10613
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6735_length_10613_cov_8.437962"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..646)
                     /locus_tag="PL162_07660"
     CDS             complement(89..646)
                     /locus_tag="PL162_07660"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HQF91929.1"
                     /translation="MSRISVLLGSPRKGGNSETMAEEILAHAPAGSSIDRIVLSSLRL
                     EGCRDCRRCWSGGTPCVIPDDMSKVYRSLDQADLIFFVTPLYWYSWSTSIKAVWDRLL
                     PYCTPSAPRSLQGKRAVLVASAGDDGTDCFDGLRFSMKKSCDLLGVSVVEEFLEGELM
                     GPEDAKRRPTLLQRLKERGGALFHP"
     gene            complement(871..1872)
                     /locus_tag="PL162_07665"
     CDS             complement(871..1872)
                     /locus_tag="PL162_07665"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HQF91930.1"
                     /translation="MTEDHRPPAPSPEVDPGDLGEEDQGPPPGDGATVVTVSLPATHA
                     KERLDRVVARELGLTRSFATKLIRRGHVALDPPERVKPSFQCGGGAVLRVILPPPQRL
                     ELEPEPVPFDVVFSDLDCIVVHKPAGVVVHPSPGHWRGTLVHGLLHAFPDIGAVNGIV
                     RPGIVHRLDAGTSGLMVVARNMWAHDALSRSFRDRRVRKRYLALIHRPPAWTRQEIRL
                     PIGRDPGHRLRMAVNADGREALTEVLVLRNLPRHALVACDLHTGRTHQIRVHLTFLGH
                     PLVGDRLYGSSYPLPEGFDRVFLHAWQLAFPHPRTGELLSFQAPLPKDLLSVLQALR"
     gene            complement(1869..4652)
                     /gene="ileS"
                     /locus_tag="PL162_07670"
     CDS             complement(1869..4652)
                     /gene="ileS"
                     /locus_tag="PL162_07670"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HQF91931.1"
                     /translation="MAKDYKDKMNLPRTSFPMRANLAAREPELLEFWKRSEVYAKLLA
                     RPSEATFVLHDGPPYANGNIHIGTAFNKILKDLIPKYKSMRGFRAPYVPGWDTHGLPI
                     ELRVLKEGGISKEQINPVELRRRCMAYASRFLDVQREEFRRLGVFGDWENPYVTFLPE
                     YEAAQLGAFADMVERGLVYKGHKPVYWCTDCQTALATAEIEYEDKKSLAVYVSYPMPE
                     AQNRFESLRGEEVSVVIWTTTPWTLPASMAVAFHGEYEYIFVREVGGKVLLLAKDLRE
                     RFSQETGLELGEVLQSCRGRDLEGLTARHPFYDKSLALVLADYVELDQGTGCVHTAPG
                     HGVDDFETGIKYGLDIYNPVDDRGYFVEGVPLVGGLSLDQGSQRVLDVLRESGRLLKQ
                     GPIVHSYPHCWRCKKPVIFRATEQWFIAVSAFRDQALQEIERVQWVPAWGEDRIANMV
                     RERSDWCISRQRIWGVPIPAFTCDGCGQVVLTPDRVRRVQEQVRTAGSDIWWSASPED
                     LLGDLAFCPSCGARHLRKETNIMDVWFDSGVSHLAVLELRPGLTWPADLYLEGSDQHR
                     GWFQTSLLTSVATRQKAPFRAVLTHGFIVDGEGRKMSKSLGNVMVPQEIIGEFGADIL
                     RLWVASTDYRNDIRISKKIVDNLVESYRRIRNTARFLLGNLYDFSPKDHQVPGDQMEE
                     MDRWVLSRLQDVVRKATEGFESYEFHVPSFIIHQFCVNDISAIFMETAKDRLYASAPG
                     DRERRSCQTVMWELLGTLTRLLAPVLSFTAEEIWQQMRKIDDCLPESVFLAPWPEARG
                     EWIDAALEERWQTILPVRSAVVRALEKARAELGIGQSLEAAVSVTVPPALEPLMASSP
                     VAFWADLCIVSAFSWSLASDTSKDTDEVEVAISRVEGDKCPRCWKYSSSPEGEEKLCP
                     RCADALRRMETSA"
     gene            complement(4759..6843)
                     /locus_tag="PL162_07675"
     CDS             complement(4759..6843)
                     /locus_tag="PL162_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HQF91932.1"
                     /translation="MDGELPGPATIDLDALRREFDTEARFRKLRGWMAGVVTGACVAL
                     SLFHLYTAGFGLLNAIKQRSVHLAIVLFVVFLIYPASQKSAKDRIPWYDFVLASMAAF
                     VVFYHVIFFQDLLLRSGLPTTMDLVAGFLGITLLLEATRRVSNPVLPIIAIIFLLYCY
                     FGRYFFGIFAHRGYNFTRIINHMYLGTEGVFGTPLEVSATFVFLFILFGTFLEQTGMG
                     KFIIDLAMALAGHSTGGPAKVAVISSGLMGSISGSSVANVCTTGMFTIPLMKSVGYKP
                     YFAGAVEAVASTGGQIMPPVMGAAGFIMATMMEVPYLEVAAAAVIPALLYYFAVYVQV
                     HFEAHRLGLRGLARERLPHIVPLMKEKGHLLIPLAGIIYFLVAGYTPLKAAFNGIMIT
                     VVVSFFRKDTRLTLPRMLAALEEGARGALGVACACATVGIVVGTATLTGLGLTIANAI
                     VKLSGGMLFLTLAFTMVACILLGAGLPTTANFIVTSTMAAPALFQFDIPPMAVYMFVL
                     YYGIAADLSPPVALAAYAGAGIAGSDPMKTGMTAMKLAIAGFIVPFIYVFNPMLVLVG
                     LPVRPAMLAILAVGLLLAGVLGAMNRENSSKKRATFGVIAALSLGVLGFLVMKDIIPL
                     DFIQAMATAMVGVCLLGMSAVGFYRAEMSWWVRLLALAGALGLMIPGWKTDLAGLVIL
                     VVIHFYQRSQEK"
     gene            complement(7224..7685)
                     /locus_tag="PL162_07680"
     CDS             complement(7224..7685)
                     /locus_tag="PL162_07680"
                     /inference="COORDINATES: protein motif:HMM:NF020479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HQF91933.1"
                     /translation="MGKVLVGLGLLFLCVIPTPVIKISHHDGTPWIEQKVLPGQEVIT
                     VYTHSVERTEVRDTYRVVGGTLWAWETWTKSQNAGLPTETGLWGRYFLRGPWQVYQGC
                     HASYCRIVWRVGNELLGRNVVMLPPGKEVKAFLLFPGERMAFAASWGLGPF"
     gene            complement(8482..8556)
                     /locus_tag="PL162_07685"
     tRNA            complement(8482..8556)
                     /locus_tag="PL162_07685"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8522..8524),aa:Ala,seq:cgc)
     gene            8754..10424
                     /locus_tag="PL162_07690"
     CDS             8754..10424
                     /locus_tag="PL162_07690"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HQF91934.1"
                     /translation="MALGSELVRAWAGALIDRHQAPDRVARLEGGREWVALQCWHGKD
                     WLFITWHADSPGLCRMAPHRMADLKSAGESPCSLLLALRAHIGGARFVRAEQWGHDRV
                     LELFFERSIGAGICRGRSLVLECAGRYSNLALRDEGGIIVDAAKHVHPDMNRYRSLLP
                     GHPYVPPPPMEGFTPEETSWIDEAPSSLTHMIGFGRPFLAHVARHWQTLPRDRWVQAL
                     REGFFAPHRGIVQELGKGYLTQFPFLLPEGTEISEDLLIASQRIVAAPMRSRILQTGL
                     RFLRQEAESVAHRLAREEEGFAHKELLFGQADQRRVWGDLLLSHAPRVEEALAMRATS
                     IALSLWESEAPPEEIPLDPERSVAANAQNCYRMYRKWKAYGQDLERERRALEARRHDV
                     EEQMAVLDAVEDIQVLRSLLEISRPGPSTKKRRPAPPHLRYDLAGGRVYVGLNAPGNR
                     YVTFEIASGDDLWCHAQNVPGAHVIAQVEDEGQRDVLVHFAASLAAAYSRAKGSLSVA
                     VDVTERRQVRHVPGQGVAFVTYHRFRTVRVSPSYWRSVPYAVVGKGPS"
BASE COUNT         2241 a         3278 c         3102 g         1992 t
ORIGIN      
        1 gacagcgcaa ggggaatgat accaccccag agattatccg tcaaggggga aagggcggcg
       61 aaaaaagggc ggaggctcca agaagtccct agggatggaa aagggccccc ccgcgttcct
      121 tgagcctttg aagcagcgtc ggccttcgct tggcgtcctc gggacccatc aactcccctt
      181 ccagaaactc ttccacgacg gagaccccca gcagatcgca ggattttttc atagaaaatc
      241 gcagtccgtc aaaacaatcg gtgccatcgt cccctgccga ggccaccaat accgctcttt
      301 tcccctgtag tgaacgggga gctgaaggcg tgcagtacgg aagaaggcgg tcccatacgg
      361 ccttgatcga ggtactccag ctgtaccagt aaagcggtgt cacgaaaaaa atgaggtccg
      421 cctgatccaa ggagcgatac acttttgaca tgtcgtcggg gatcacgcag ggcgttcctc
      481 cactccagca gcggcgacaa tctcggcatc cttccaggcg gagcgaggag agaacaatgc
      541 gatctatgga gctccccgcc ggggcgtggg ccaagatctc ctccgccatg gtttcgctgt
      601 tgcccccttt tcgagggctc cccaggagaa cggaaatgcg actcatgttc ttaccccctt
      661 tcttgacctt ctccggatct cgctttggca ctggggcacc ttgatttttt tcgccccggc
      721 ccgccatcgg cggtctttcg caaaaaaccc tccctaagac ggcggaaagc gacgtcacga
      781 caagaccttg cccgagccga cgctccgacg aacgcgacgg tttagtgtag tgtcgacatc
      841 cccattctat catatatatc tcggggattc ctagcgcagg gcttgcaaaa ccgagagtag
      901 atccttcggc aaaggagctt gaaaactcag aagctccccc gtgcgggggt gaggaaaggc
      961 cagctgccaa gcgtgcaaga atacccgatc aaagccttcc ggcagcggat aggatgagcc
     1021 gtacagccgg tctcccacca aagggtgtcc cagaaaagtc aggtggacgc ggatctgatg
     1081 cgtccttccg gtgtgtaggt cgcaggccac cagggcgtgg cgagggagat ttcgaagcac
     1141 caaaacctcc gtgagggcct cccgtccgtc ggcgtttacc gccatgcgaa ggcggtgtcc
     1201 cgggtctcgg ccgatgggaa ggcgaatttc ctggcgcgtc caagccgggg gacggtgaat
     1261 caaggcaagg tagcgtttac gcacgcgccg atcccggaaa gaccgggaaa gggcgtcgtg
     1321 ggcccacatg tttcgggcca ccaccatcaa ccccgaggtt cctgcgtcca aacgatgcac
     1381 gattcccggg cgcacgatgc cattaacggc tccgatgtcc gggaaggcat gtagtaggcc
     1441 gtggaccagc gtgcctcgcc aatgccccgg ggaggggtgc actaccaccc cggcgggttt
     1501 atgtacgaca atgcagtcta aatcggaaaa caccacgtca aaggggaccg gctcgggctc
     1561 tagctccagg cgctgcggag gggggaggat cactcggagc accgcccccc ctccacactg
     1621 aaaagaaggc ttgacccgtt ccggagggtc caaggccaca tgtccccggc gaatcaattt
     1681 ggtggcgaag gagcgagtca gccccagctc ccgggccact acccgatcca acctctcctt
     1741 cgcatgggtg gcgggtaggg agacggtcac caccgtagcc ccatcgccgg gggggggccc
     1801 ctggtcttct tcccccagat ccccaggatc cacctccggc gatggggcgg gggggcgatg
     1861 gtcttccgtc acgcggaggt ttccatgcgg cgcaaggcgt ccgcacagcg gggacagagt
     1921 ttttcctccc cctcggggga ggaagagtat ttccagcaac gagggcactt gtcgccttcc
     1981 acccgggaga tcgccacttc gacttcatcg gtgtccttcg acgtgtcgga ggcgaggctc
     2041 catgagaagg cggagacgat gcacaagtcg gcccagaagg ccaccggaga cgatgccatg
     2101 aggggctcca gcgccggagg caccgtcacg gaaaccgccg cttccaagga ttgaccgatg
     2161 cccagctcgg cgcgggcctt ctccagggcc cggaccacgg cgctcctcac cgggagtatg
     2221 gtctgccatc tctcctccag agcggcatcg atccactcac cccgggcctc gggccaggga
     2281 gccaggaaga cgctttcggg caggcagtca tcgatttttc tcatttgctg ccaaatttct
     2341 tctgcggtga agctcagcac gggggccagg aggcgcgtca aggtccccaa aagctcccac
     2401 atcaccgtct gacagctccg gcgctcccta tcccctggcg cgctggcata aaggcgatct
     2461 ttggcggttt ccatgaagat ggcgcttatg tcgttgacgc agaactggtg gatgatgaag
     2521 cttggcacgt ggaactcgta gctttcaaaa ccctccgtgg ccttacgcac cacgtcctgg
     2581 aggcgggaga gcacccagcg atccatctct tccatctggt ccccggggac ttggtgatcc
     2641 ttcggggaga agtcgtagag gtttcccagg agaaaccggg cggtgttgcg gatacgccga
     2701 taggactcca ccaagttgtc gacgatcttc ttagagatcc ttatgtcgtt gcgatagtcc
     2761 gtggaggcca cccagagacg caggatatcg gctccgaatt cgccgatgat ctcctggggc
     2821 accatgacgt tccccaggga cttggacatc tttctcccct ctccgtccac gatgaagccg
     2881 tgggtcagga cggcccgaaa aggggccttt tggcgggtgg ccacggaggt gaggagcgat
     2941 gtctggaacc acccccggtg ctgatcgctt ccctccaagt agaggtcggc gggccaagtg
     3001 aggccagggc ggagctcgag caccgccagg tggctcactc cggaatcgaa ccacacgtcc
     3061 atgatgttcg tttccttgcg aagatgtctg gcaccgcagg agggacaaaa ggccaagtcc
     3121 cctagtagat cttccggtga cgcgctccac catatgtcgc tgcccgccgt gcgtacttgc
     3181 tcctgaaccc ggcgcacccg atccggagtg agcaccacct ggccacaacc gtcgcaggtg
     3241 aaggcgggga tggggacgcc ccaaatgcgc tggcggctta tacaccagtc tgagcgttcc
     3301 cgcaccatgt tggcgatgcg atcttcaccc caagcgggga cccattgtac ccgctcgatc
     3361 tcctgcaatg cctggtcgcg aaaggcgctc acggcgatga accactgctc cgtagcccgg
     3421 aatataaccg gttttttgca ccgccagcag tggggatagg agtgcacgat gggaccttgc
     3481 ttcagcaatc ggccggactc tcgcaggaca tccaacactc gctgagatcc ctggtccagg
     3541 gaaaggcctc ctaccagggg aacgccttcc acgaagtacc cccggtcgtc cacggggttg
     3601 tagatgtcca ggccatattt tattccggtc tcgaagtcgt ccaccccatg ccccggagcg
     3661 gtgtggacgc accccgtgcc ttggtcaagc tccacataat ccgccaaaac caaggccaag
     3721 gacttgtcgt agaacgggtg gcgggccgtc aatccttcca gatcccggcc tctgcagctc
     3781 tggaggactt ccccgagctc cagccccgtc tcctgggaga agcgttcccg gagatccttg
     3841 gccagaagga gcacctttcc ccctacctcg cggacgaaga tgtattcgta ttccccgtgg
     3901 aacgccacgg ccatgctggc gggcagggtc cagggtgtgg tggtccagat taccaccgaa
     3961 acttcttctc ctcgtaggga ttcaaagcgg ttttgagcct ccggcatggg gtagctcaca
     4021 tagaccgcca aagatttttt gtcttcgtat tcgatctcgg ccgtcgccag ggcggtctga
     4081 cagtccgtac accagtaaac gggtttgtgt cccttgtata ccaatccccg ctccaccatg
     4141 tccgcaaagg cccccagctg ggccgcttcg tactccggca ggaaggtcac atagggattt
     4201 tcccagtctc caaagactcc caggcgccgg aactcctccc tttggacgtc caggaagcga
     4261 gaggcatacg ccatgcatcg ccgccgtagc tccaccggat tgatctgctc cttggaaatg
     4321 cctccttctt taagcactcg cagctctatg ggcaagccgt gggtatccca ccccggcacg
     4381 tagggcgccc gaaagccccg catgctcttg tacttgggga ttaagtcctt gagaatttta
     4441 ttgaaggcgg taccgatatg gatgttgccg ttggcgtagg gggggccgtc gtgcaacacg
     4501 aaggtggcct cgctgggacg agccaggagt ttggcgtaaa cttcggatct tttccagaac
     4561 tccagaagct ctggttcccg ggcggctaaa ttggccctca tggggaaaga tgtccgggga
     4621 aggttcattt tatccttata atcttttgcc ataaagcatc ctccttgaga aaggtctgtt
     4681 gccgccctgg cctaggcgag ggcggcaacc acgagggtct tcccgggggt cacatcgcca
     4741 gaaccatcgc cgatgggatc acttttcctg ggacctttgg tagaagtgga ttacgacgag
     4801 gatgacgagc ccggccaggt ccgttttcca tccggggatc atgagcccca gggccccagc
     4861 cagggccaaa aggcgcaccc accaactcat ctccgcgcgg tagaacccca cggcgctcat
     4921 gcccaggagg cagacgccca ccatggccgt ggccatggcc tggatgaagt ccagggggat
     4981 gatgtctttc atcaccaaga accccaggac tcccagggaa agggccgcta tgacgccaaa
     5041 ggtggcgcgc tttttcgacg aattttcccg gttcatggcc cccaaaactc cagccaggag
     5101 gagccctacg gccaggatgg ccagcatggc ggggcgcacg ggaagcccca ccaggaccag
     5161 catggggtta aatacgtaga tgaatggaac gatgaagccc gcaatggcga gtttcatggc
     5221 agtcatgccg gtcttcatgg ggtcggaccc cgcgatgccc gcccccgcat acgccgccag
     5281 cgccacagga gggctcaggt ccgccgcgat gccatagtac agcacaaaca tgtagaccgc
     5341 catggggggg atgtcgaatt ggaagagggc gggggctgcc atggtgctcg taacaatgaa
     5401 attcgccgtg gtgggcaatc ccgctcccag gagaatacag gccaccatgg taaaagccaa
     5461 cgttaaaaac agcatgcctc cagacagctt aacgatggcg ttggctatgg taaggcccaa
     5521 tcccgtcagc gtggcggtgc ccaccacgat gcccacggtg gcgcaggcac aggctacgcc
     5581 cagggctcca cgggcccctt cttccagggc ggccagcatg cggggaaggg tcaaccgggt
     5641 gtctttccgg aagaaggaga ccaccactgt aatcatgatc ccgttaaatg cagccttaag
     5701 aggggtgtac cccgccacga ggaagtaaat gatccccgcc aggggaatta gcaagtgtcc
     5761 cttctccttc atgagcggca caatatgagg caagcgttcg cgggccaacc cccgaaggcc
     5821 cagacgatgg gcttcgaagt gtacctggac gtagacggcg aagtaataaa gaagggctgg
     5881 gattaccgcc gcagcggcca cttccagata ggggacctcc atcatggtgg ccatgatgaa
     5941 cccggcggct cccatcaccg ggggcatgat ttgtcctccg gtggaggcca ccgcttccac
     6001 ggctccggca aagtagggtt tgtatcctac acttttcatg agaggtatgg tgaacatccc
     6061 cgtggtacaa acattggcca ccgaggagcc cgaaatggaa cccatgagtc ccgagctgat
     6121 taccgccacc ttggcgggcc ctccggtgga atgccccgcc agggccatgg ccaggtcgat
     6181 gatgaatttc cccatgcccg tctgttccag aaaggtccca aacaagatga acaggaacac
     6241 aaacgtggcg gaaacttcca ggggagtacc aaagacccct tcggtgccca gatacatgtg
     6301 gttgattatt ctagtgaaat tgtatcctcg atgggcaaaa atgccgaaaa aatatcggcc
     6361 aaagtaacag tacagcaaaa agattatggc gatgatgggc aagaccggat tcgacacccg
     6421 gcgagtggct tccaaaagga gtgtgatgcc caaaaaaccc gccacaaggt ccatagtcgt
     6481 cggaagcccc gaccgcaaga gcaggtcctg aaagaaaatc acgtggtaaa atacgacaaa
     6541 ggccgccatg gaggccaaga cgaagtcata ccatgggatg cggtcttttg ccgatttctg
     6601 cgacgcggga tagatgagaa acaccacaaa aagaactatg gccaggtgga cggagcgctg
     6661 cttgatggcg ttgagcaagc cgaaaccggc cgtgtagagg tgaaagagag acagagccac
     6721 gcaggccccc gtgacaactc ccgccatcca tccccgcaac ttgcgaaaac gggcctcggt
     6781 gtcgaattcg cgacgcaggg cgtccaggtc tatggtcgca gggccaggga gctcaccgtc
     6841 catgacctga tgttgctttt gttccggtgc aggttttccc aaatccatcg cctccttttc
     6901 tgatcaatcc ttacggtcct ttcgcccacc gttctgcggc cgatgcccct tggaaaggcc
     6961 tctccctgtt tcaaccccgg aagatccttc caaaaaaggc gccttttccc ccccttccct
     7021 ctccccttgc cgtactctcc cagggttcca gggtaaactc cctgcagagg tcaccgcagt
     7081 ctccatcgac gaaggtggaa tctgatgagg aatcaccgca gggacggccg tatctttttc
     7141 tagattgccc ttcctccccg aaaaataggc caccaaaaac gctttaggaa ggaactcctt
     7201 gaaagggagg ggcctttaaa aaactagaaa gggcccaacc cccaagaagc cgcaaaggcc
     7261 atcctctccc ccggaaaaag aaggaaggcc ttcacctcct tccccggtgg gagcattacg
     7321 acgtttctcc ccaagagttc gttccccacg cgccacacga tgcggcagta gctcgcatga
     7381 cacccctggt aaacctgcca cgggccccgg aggaaatagc gtccccacag cccggtttcc
     7441 gtgggtagtc ccgcattttg agattttgtc caggtctccc aggcccacaa cgtccctccg
     7501 acgacccggt aagtgtctcg cacctcggtg cgctccacag agtgggtgta tacggtaatc
     7561 acttcctgcc ccgggagcac cttctgttct atccacggag tcccatcatg gtgggaaatc
     7621 ttgatcaccg gcgtggggat gacgcagaga aagaggagcc ccagccccac gaggaccttc
     7681 cccaacccct tttcccaccg acgcagggcc tttccaaaga agacctcccc tcgcggcccc
     7741 ccataagggc ccgacgatcc tgctttccaa aagtccgccc ccatttcatc gggccccttt
     7801 ccccagatcc cccttctctt gtctccccat ggaatacccc tcccaagtct atcacggaag
     7861 gaaggatgtt gaaagggacg gctccgtgac tcccggtcct ctggggtgga aggagcggag
     7921 ggctgagaaa taccttcgga tacaaaaacc accactcctc cggacggtct ccagcccaaa
     7981 agaagtggtg gaacccttgg atcaacccat cgttcctatg accctttccc gacgaaaaac
     8041 tgaggagcga ttcccgaagg gagccctaga ccgcttcccc tcccttctca cgcagatagg
     8101 ccatcggcac gcggagcaca caacggcgcg tgcttctggt ccacggagtg cggaaaaaat
     8161 aaagggcggc cgtgaacgcc tggcgcgggt tttctcaccg cctaaggtac cacttctcga
     8221 aggaatcggg aatgtgccga tcgaggtccg gggttgacgg agcaaggccg aggccccggc
     8281 ggaagatgtg gaagcttccg acggcggcac gtagaagcgt cgcctcctct tgtgccgctc
     8341 ttgcagattt tctaaccaca aaacacgcct ccccccagga tagaaaatca ccccaggaag
     8401 aggctggttt ctaccgcgaa gccgccactc ccttggcgag actttcgcgc tgtaaaaggg
     8461 tctctaaacc cttgtaattg ttggtggagc tggggggact cgaacccccg gcctattcct
     8521 tgcgaaggaa tcgcgctccc tctgcgctac agccccacgc cacaccacaa cccgggaact
     8581 attccaaaac gccagggcca cggtccccca gccatatcgg atgccacaac ccggcgcaac
     8641 ccggggagta ttctagtgcg gcacccccgc tcttgtcaag agtgcgtggt tccaggtaga
     8701 ctgttccttc gtgctcttct aagggtatga cacgacggag agggtgggga aaaatggccc
     8761 tgggatcgga attggtcaga gcttgggcgg gggcattgat agatcggcat caagccccgg
     8821 accgggtcgc ccggctggaa ggggggcggg agtgggtggc cctccaatgt tggcacggga
     8881 aggattggct ctttataaca tggcatgcgg attcccccgg actgtgtcga atggcgcccc
     8941 atcgcatggc ggatttgaag agcgcgggag aatccccctg ctccctgctt ctggcgctgc
     9001 gtgcccacat tggcggggcc cgtttcgtac gcgctgagca gtggggacat gaccgcgtgc
     9061 tggagctttt tttcgaacgc tccatcggcg ccggaatctg tcgagggcgt tctctggtgc
     9121 tggagtgcgc cggacgctac agcaatctgg ccctccggga cgagggggga atcatcgtcg
     9181 atgcggcaaa acacgtccat ccggatatga accgttaccg gagccttctg cccggacacc
     9241 cctatgtccc ccctcccccc atggagggat ttacccctga agaaacttcg tggatcgatg
     9301 aagccccttc ttctctgact cacatgatcg gcttcggccg tcccttcctg gctcatgtgg
     9361 cccgccattg gcagaccctt ccccgggata ggtgggttca agccctgcgg gagggttttt
     9421 ttgcgcccca tcgaggcatc gtccaggagc taggcaaggg atatctgacc caatttccct
     9481 tccttctccc tgaaggcacc gagatctccg aagatctcct catcgcctcc cagcggatcg
     9541 tcgccgcccc catgaggtcg cgaattttgc agaccggact ccgcttcttg cgacaagaag
     9601 cggagagtgt ggcccatcga ttggcccgag aggaggaggg ttttgcccac aaagagctac
     9661 ttttcggcca ggcggatcag aggcgagtct ggggagatct cctgctctcc cacgccccca
     9721 gagtggagga ggcgctggcg atgcgggcga caagcatcgc tttgtccctg tgggaatcgg
     9781 aagccccgcc ggaggagatc cctctcgatc cggagaggag tgtagccgcc aacgcccaga
     9841 actgttaccg aatgtaccga aagtggaagg cctacggcca agatctcgag agagaacggc
     9901 gcgccctgga ggcccggcga cacgacgtgg aggagcagat ggccgtgctc gacgcggtgg
     9961 aagatattca ggtgctccgg tctctcctgg agatctcccg ccccgggcct tcgacaaaaa
    10021 agcgccgccc tgccccacca catcttcgat acgatctggc cggggggagg gtctacgtgg
    10081 ggttaaacgc ccccgggaat cgatatgtca cgtttgagat agcctcgggg gatgatctct
    10141 ggtgccacgc ccaaaacgtc cccggggccc acgtcatcgc ccaggtggaa gacgaagggc
    10201 aacgggacgt cctggtccac tttgccgcct ctttggcggc ggcctacagc cgggccaagg
    10261 gaagcctttc cgtggcagtg gacgtgaccg aacgtcgcca agtgcgtcac gttcccggtc
    10321 aaggggtggc cttcgtcacc tatcaccgtt ttcgtaccgt tcgcgtctcc ccgtcttact
    10381 ggcggagcgt tccctacgcc gtggtgggaa aaggaccaag ctgaagcgcc ggagccaaaa
    10441 aagcccccca aaagggggag cttttgcgcc aaggcttcgg gagaatctcc ctaaggggag
    10501 gggacggtag acagggtgga tttcccgtac cgccccatgt gggcctctgc gtccagagcg
    10561 tctagaaagc cccaggaagc gaagaccatc tcccgcaccg agagcccctt tcg
//
LOCUS       DAOOER010000061        10262 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_7013_length_10262_cov_8.510238, whole genome shotgun sequence.
ACCESSION   DAOOER010000061 DAOOER010000000
VERSION     DAOOER010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 10262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10262
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7013_length_10262_cov_8.510238"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="PL162_07695"
     CDS             <1..548
                     /locus_tag="PL162_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HQF91935.1"
                     /translation="VHKVSIIPTAKGALGYTLQMPEEDKFLLGEEELRSRMAVMLGGR
                     AAELLVFGEATTGAANDLERVTELARRMVTEFGMSPRLGPVRYAAAAGAYLGGGVASR
                     TDLSPETVEIIDGEIRALVTSAQDRALEVLREHRSVLDAVAEELISREAITGDRIREI
                     AAEKREEALPAAPMPEEPLRS"
     gene            complement(715..1575)
                     /locus_tag="PL162_07700"
     CDS             complement(715..1575)
                     /locus_tag="PL162_07700"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HQF91936.1"
                     /translation="MKKTWLWIKRAAAMAVVLGMAFVPEARGEDWTYEAYTRAVQESG
                     RSMDISQKTFDKVQGKREKALEAIEKYLTSRFGAADPEVLRAFREVPREYFHHNYQSG
                     RGMASVAYEIPAKPWGIGYGSALSDYLGQAYMTQLAGVKSSDVVLEIGTGSGFQISLF
                     SRIAREVYSIEIIEPLGEGVGKIFAPLGYANVHTRVGDGYFGWPEVEEGFDVIMLTCA
                     AQYASPELLKQLKPGGKLIIPIGQPFKKGQFLYVFTKDAEGKVRSKKDMGVYFIPMTG
                     EIQKRKQEKQ"
     gene            complement(1670..4108)
                     /locus_tag="PL162_07705"
     CDS             complement(1670..4108)
                     /locus_tag="PL162_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91937.1"
                     /translation="MSHRFRAWGGLFFVSLASIAFEIFLTRFFALSNWSEYGYWVISM
                     AMAGYALSGVVLSLGSAWAERHREGLLFICALILPAAAAGGYVLVTLNPFNPLEFQNA
                     VLWKDQLGHIGMYYVALFPFFFVSGIFIGLSFLTGRREIPRLYMVDLVGAGAGALITL
                     GGLFLLHPFSLMTPFLGLLVPPILLTAPRGLSRSTVLGGAGALVALCALGASLGARPA
                     LCPYKALASALNVEDRRIEREIRTPEGLYLLVSSYGERLDADLSNNYELLDAGGPPDA
                     VGIYRDGDRLSALPRRGSFDLSYLRAGLDSLPYALRPGARTLLLGSRGTFRLREAFAL
                     GASTVTALETDPVLQGLVKDSLGAPGGYLGAVTLKDAPPYALDPREASFDVVDVSLEF
                     LDQGAANKYVLTEEGVAHLLSLLSPRGVLSLPVAIGEFTVYAVKAVATVAAALERAGI
                     PSPPRHILVYRSSWNARILVSPTPWTPEDVAHFRSFCSDRSFDTAYFFGMDPAALEVW
                     NDLPPVSFEEEAVSGGEGPQDALRDDLGAFLDRGERSSSSWTFFDLSPATFDRPFFFG
                     ILRPQKISAVLDRMDILPRPEIAMLINGAVLSQALFFALVVLALPLIRRKRLELSGGE
                     IFRGMGYFACLGLGFLAVEMALIERATFYLGDPAWAFALVLASMLIFSGMGSLLAEKA
                     FSDPRRGAPAAAAGACALVILLSWGLMPLFSATLSWAPGAKRLLVVLVAALPSLALGM
                     PFPQGLSWFGGRSPAFLPWAWGINGAFSVIATPLAALAAALGGYRWVFMGAALLYALG
                     ALAFPRGKGGTDHS"
     gene            4267..5187
                     /locus_tag="PL162_07710"
     CDS             4267..5187
                     /locus_tag="PL162_07710"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HQF91938.1"
                     /translation="MDMLRDSDLYRRVLESAYDGIYVTDLHRKIVYWNQGAERLSGYK
                     AEEVVGRSCADNVLVHVDGQGNNLCLGMCPLAHTMQDGEMREADVFMHHKEGHRVPVS
                     VRVTPVTDGEGRIVGGIEIFSDNSTKMAALELADTFQEIALTDTITGVGNRRYTEISL
                     LARIDELQKDGVPFALLVGDLDGFKRVNDDFGHLAGDQVLRSVAQTLQGGLKSFDFLG
                     RWGGDEFMMIFPNIRDKKTLEELGERLRMLVEKSFLLWDGHRIEGTMSLGGTLGRPED
                     RSQDVFSRADRALYQSKEGGKNCVTVLVAP"
     gene            5237..5944
                     /locus_tag="PL162_07715"
     CDS             5237..5944
                     /locus_tag="PL162_07715"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HQF91939.1"
                     /translation="MRFSIDPSVFELFPEVGLGVVQARHVDNSRDDPEVVEILRRAER
                     DLPRIFGEGPVADHPRVSVWREAYRKFGAKPKATPSSVENLVRRVLGGELLRPVNNLV
                     SLYNAVSLTHLLPVGGEDRDRLSGDVRLTRAGPAEPPVILLGEKDPRSPHEGEVIYTD
                     DIGALCRRWNWKEADRTKLTPETKNVILVIESLPPLPPDALPKAADDLAALVERHLGG
                     TVSTLLLHRGNPEGILR"
     gene            complement(5989..7890)
                     /locus_tag="PL162_07720"
     CDS             complement(5989..7890)
                     /locus_tag="PL162_07720"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HQF91940.1"
                     /translation="MGPRALSLLIFFVVALSLPAEGVLRVVGDRNFPPFMFLDERGEP
                     SGMDAAVARLVGERMGEEVSIDLLPWAEAVLALEIGSADILAAMRVTPEREGRYRFAF
                     PHCVNKLTAFTRVAATHINTSADLWGRRVGVQRRDLAEDFCRQYLSESDLWLYDDQPQ
                     ALRALADGEVDAFVGNEAVVYYTIARDHLTDLFRALEAPLLSSPYAMAMRSDDRAMAA
                     AVNKALTILKQDGTLLQLQQRWTRALPQNAALRRQLLYTALGAAFLGGVGVLGMMILG
                     WRVRRATRHLEEEKELLRREVEERHRVERDIERQRRFISAVLEHVPEAILVKDLSSGR
                     YTMLNRAVETLFGLPRDHLEGRTDEEIFGLTPGEIFAAQDREVAQSSQAHTFPEVPVL
                     TPHRESRLFTFTKTLLPPSPDDPSDHLLAIVQDVTERRRAEEALRFASFHDALTGLHN
                     RGYFEEEMRRLATGRFNPVGLLVCDVDGLRIVNNVAGHAAGDTLIRGAAEVLRRTFRS
                     SDVVARIGGDEFAILLPQCPGPVAQSRCRDLARHVEEWNASSPLYLGLSLGLAHHQEG
                     GKPSMERLFREADQAMYRHKEATREEVMEKVFARVASLLGEGEDDPVARRQWEDLRSR
                     WDTRGSSRG"
     gene            8251..8982
                     /locus_tag="PL162_07725"
     CDS             8251..8982
                     /locus_tag="PL162_07725"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HQF91941.1"
                     /translation="MRAHVFKACLALCMVLIMGAALQERFPIHRPTTIFHGGGRIYAP
                     MVIRMMSPAVDAELRAMGASRRHLLEWRDRHPTAALRHLWSMEEEDQRTCARIARYIL
                     RQNRAITPELAWVEAAALVHYSAKYGAPVELAVAVANTESHFSPESLSSYGAMGVMQV
                     VWRIHHGLLQANGILSGEELHNPDRGIAAGCLLLGRYLRAEGDTREALGRYAGGSVEA
                     YCGKVEGYVDRFRRLVAEGGTSRGM"
     gene            9266..9904
                     /locus_tag="PL162_07730"
     CDS             9266..9904
                     /locus_tag="PL162_07730"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HQF91942.1"
                     /translation="MAKKIAPTRGNLLRVLRALHLARKGHDLLEQKRQVLMMELMRHI
                     DRARDIQKDMAEIFAAAYRALQHANVSVGIDTVQEISRAVPESDEFIVRLRSVMGVDI
                     PEVDPIPEACPPGYSFQGTSGAVDRAFLAFHRVRVLVARLAEVETSVYRLAVQIRKTH
                     RRVNALEKVVIPQYGAAAVAIGAVLEEGDREDFARMKISKEAGKKGLGREVP"
     gene            9969..>10262
                     /locus_tag="PL162_07735"
     CDS             9969..>10262
                     /locus_tag="PL162_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HQF91943.1"
                     /translation="MAALQEVLAVLLGAEGEARSEIEESRNEAERVVRRAKDAFSQER
                     ERRLAAAREQAKAVLDLARSAADAEGGQILQMGQQDLDRMRERFDENVQGLVAT"
BASE COUNT         1817 a         3165 c         3636 g         1644 t
ORIGIN      
        1 ccgtgcacaa ggtgagcatc atccccacgg ccaagggggc cctggggtac acgctccaga
       61 tgcccgagga ggacaagttt ctcctggggg aggaggagct ccgttcccgc atggcggtga
      121 tgctcggcgg acgggcggcg gagcttttgg tcttcggcga ggccaccacg ggggcggcga
      181 acgacctgga gcgggtgacg gagctcgccc ggcgcatggt gacggagttc ggcatgtccc
      241 cccggctggg gcccgtgcgc tacgccgccg cagcgggggc ctatctgggg ggaggggtgg
      301 ccagccggac cgacctgagc cccgaaaccg tggagatcat cgacggggag atccgcgccc
      361 tggtgacctc cgcccaggat cgggccctgg aggtgctccg ggagcaccgg agcgtcctcg
      421 acgccgtggc ggaagagctc ataagccggg aggctatcac gggggaccgc atccgggaga
      481 tcgcggcgga gaagcgggaa gaagctcttc ccgccgcgcc catgccggag gaaccgcttc
      541 ggagctgatc ttttgcacgt cgcgtttcct gccgatgctt tcgaggaaat ccctctactg
      601 gttgtagggg cgaaaaatac gctcgcccct acagtgtggt gtatcgtttt ctcttgtttc
      661 gatgcaaaaa catgcctttc ggaaagcttt cttcccctga ccttgtggtt cctcttattg
      721 cttttcctgc ttgcgctttt ggatttctcc cgtcatgggg atgaagtaca cccccatgtc
      781 ctttttcgag cgcaccttgc cctccgcgtc cttggtgaag acgtagagaa actgcccctt
      841 cttaaagggc tgaccgatgg ggatgatgag ctttcctccg ggcttgagct gcttcaggag
      901 ctcgggggag gcgtactggg cggcgcaggt gagcatgatg acgtcgaagc cctcctccac
      961 ctcgggccat ccgaagtacc cgtcccccac ccgggtgtgg acgttggcgt atcccagcgg
     1021 ggcgaagatc tttcccaccc cttcccccag gggctcgatg atctcgatgg agtacacctc
     1081 ccgggcgatg cgggaaaaga gggatatctg aaatccgctt ccggtgccta tttccagcac
     1141 cacgtccgag gacttgaccc cggcgagctg ggtcatgtac gcctgcccca ggtagtccga
     1201 tagggccgat ccgtagccga tgccccaggg cttggcgggg atctcgtagg cgacggaagc
     1261 catgccccgg ccgctctggt agttgtggtg gaagtactcc cggggaactt cccggaaggc
     1321 ccggagcacc tcgggatcgg cggcgccgaa gcgggaggtg aggtattttt cgatggcctc
     1381 cagggccttc tctcgcttcc cctggacctt gtcgaaggtc ttttgagaaa tgtccatgct
     1441 gcgaccgctc tcttgcacgg cccgggtgta ggcctcgtag gtccaatctt cgccccgggc
     1501 ttccgggacg aaggccattc ccaacaccac ggccatagct gcggcccttt ttatccataa
     1561 ccaggttttt ttcacgtcat tccctccatg tgagatgtcc ctatgggcgg catcgccgcg
     1621 ccgccggcgg atattccgga cggggatttc cccgcccgtc aaaccccttt taggagtggt
     1681 cggtcccccc ctttccccgg gggaaggcca gggctcccag ggcatagagg agggccgccc
     1741 ccataaagac ccaccgatat ccccccaggg ccgccgccag ggccgccagg ggggtggcga
     1801 tgacggaaaa ggccccattg atcccccagg cccagggaag aaaggcggga gagcgccccc
     1861 cgaaccagga gagcccctgg ggaaagggca tgcccagagc cagggagggc agggccgcca
     1921 cgaggaccac caggaggcgc ttggccccgg gggcccagga aagggtggcg gaaaagaggg
     1981 gcatgagccc ccaggacagg aggatcacca gggcgcaggc cccggcagcg gcggcggggg
     2041 ctccccggcg ggggtcggag aaggcctttt ccgccaagag gctccccatc ccggagaaga
     2101 tgagcatgga agccagcacc agggcaaagg cccaggcggg atcccccagg tagaaggtgg
     2161 cccgttcgat gagggccatc tccacggcga gaaagcccaa ccccaggcag gcgaagtacc
     2221 ccatcccccg gaagatctcc cctccggaga gctccaggcg cttccggcgg atcaggggga
     2281 gggccaacac caccagggcg aaaaagaggg cttgggatag caccgcgccg ttgatgagca
     2341 tggcgatctc cggacggggg aggatgtcca tgcggtcgag cacggcggag atcttctggg
     2401 gacgcaggat gccgaagaag aatgggcggt cgaaggtggc gggggagagg tcgaagaagg
     2461 tccaggagga ggaagagcgc tccccccggt cgaggaaggc cccgaggtcg tcccggaggg
     2521 catcctgggg gccttcgccc cccgagaccg cctcttcctc gaaagacacc ggcggcaggt
     2581 cgttccagac ctccagggcg gcggggtcca tgccgaaaaa gtacgccgtg tcgaaggatc
     2641 ggtcggagca gaaggagcga aagtgtgcca cgtcctcggg ggtccagggg gtggggctca
     2701 ccaggatgcg agcgttccac gaggagcgat agaccaagat gtggcggggg ggggagggta
     2761 tgccggcgcg ctccagggcg gcggcgacgg tggccacggc cttgacggcg tagacggtga
     2821 attctccgat ggccacgggt agggagagca ccccccgggg ggataggagg gagaggaggt
     2881 gtgccacccc ctcctcggtg agcacgtatt tgttggcggc cccttggtcg agaaactcca
     2941 gggagacgtc caccacgtcg aaggaagcct cccgggggtc cagggcgtag gggggcgcat
     3001 ccttcagggt gacggctccg aggtaccctc cgggggcgcc caaggagtct ttcaccaggc
     3061 cttggagcac ggggtccgtc tccagggccg tgacggtgga ggcccccagg gcgaaggcct
     3121 cccggaggcg aaaggtcccc cggctcccca gcaggagggt ccgggccccg gggcgcaggg
     3181 cgtaggggag ggagtcgagc cccgcccgga ggtaggatag gtcgaaagag cccctccggg
     3241 ggagggcgga gagccggtcc ccatcgcggt agatgcccac ggcgtcgggg ggccccccgg
     3301 cgtccaggag ttcgtagttg ttcgacaggt ccgcatccag gcgctccccg tagctcgaca
     3361 ccaggaggta gagtccctcg ggggtgcgga tttcccgctc gatgcggcga tcttccacgt
     3421 tgagggcgct ggcgagggcc ttgtaggggc aaagggccgg gcgggccccc agggaggctc
     3481 cgagggcgca gagggccacc aacgccccgg cgcctcccag caccgtgctc cgggagaggc
     3541 cccggggggc ggtgagcagg atggggggca cgaggagccc caggaagggc gtcatcagag
     3601 agaaggggtg gagtagaaag agccctccca aggtgatgag ggcccccgcc cccgccccca
     3661 ccaggtccac catgtagagc cgggggatct cccggcggcc ggtgagaaag gacaggccga
     3721 tgaagatccc cgacacgaag aagaagggga aaagggccac gtaatacatg ccgatgtgcc
     3781 cgagctggtc cttccacagg acggcatttt gaaactccaa gggattgaag gggttgaggg
     3841 tcaccaggac ataccccccg gcggcggcgg cggggaggat cagggcgcag atgaaaagta
     3901 gcccctcccg atggcgttcg gcccaggcgc tccccaggct cagcaccacc cccgacagag
     3961 cgtaacccgc catggccatg gagatgaccc agtagccgta ttcggaccag ttgctcaggg
     4021 caaagaagcg ggtgaggaag atttcgaagg cgatggaggc cagggagaca aaaaaaagcc
     4081 ctccccaggc ccgaaagcgg tgggacatgt gccattcctc cttgggcggc ccggagggcg
     4141 cgtcaatcct ccgaactctt gtggaccatg ataccacagg cgggccccat cccccccggt
     4201 gattctccga ggggcttgtg ataaaatgat acagaaggca tcattttttt gggggagtga
     4261 aagcgcatgg acatgctgcg ggattccgac ctgtaccgcc gggtgctgga aagcgcctac
     4321 gatgggatct acgtgaccga cctccaccgc aagatcgtct actggaacca gggagccgag
     4381 cgcctctcgg ggtacaaggc ggaagaggtg gtggggagga gctgcgcgga taacgtcttg
     4441 gtccacgtgg atggccaggg gaataacctc tgcctgggca tgtgccctct ggcccacacc
     4501 atgcaggatg gagagatgcg ggaagccgac gtgttcatgc accacaaaga ggggcaccgg
     4561 gtgcccgtat cggtgcgggt cactccggtg acggacggcg aagggcgcat cgttgggggc
     4621 atcgagatct tttccgacaa cagcaccaag atggccgcgc tggagctggc ggacaccttc
     4681 caggagatcg ccctcacgga caccatcacc ggagtgggga accgtcgcta cacggagatc
     4741 tccctcctgg cccggatcga cgaactccag aaagacgggg tgccctttgc cctcctcgtg
     4801 ggggatctgg acgggttcaa gcgggtcaac gacgactttg gccatctggc gggggaccag
     4861 gttctccgga gcgtcgccca gaccctccag ggggggctca agagcttcga cttcctggga
     4921 cgctggggag gggacgagtt tatgatgata tttcccaaca tccgggacaa aaagaccctg
     4981 gaagagctgg gggagcggct acgcatgctg gtggaaaaat cttttctcct ctgggatggt
     5041 catcggatcg aaggcaccat gtccctgggg ggcaccctgg ggaggcccga agacagatcg
     5101 caggacgtct tctcccgggc ggatcgggcc ctgtaccaga gcaaggaagg gggaaaaaac
     5161 tgcgtcaccg tccttgtggc cccctaaggg atcctttaag gcgagtggaa aagcccgggg
     5221 aaggggagtg aaggctttga ggttttcgat cgatccgtcg gtttttgaac tctttcccga
     5281 ggtgggattg ggagtggtgc aggcccgcca cgtcgacaac tcccgggacg atccggaggt
     5341 ggtggaaatc ctccgccggg cggagcgcga cctgccccgg atcttcggcg aggggcccgt
     5401 ggcggatcat ccccgcgtct cggtctggcg ggaagcctac cgtaagttcg gcgccaagcc
     5461 caaggccacc ccttcctccg tggagaacct ggtgcgacgg gtccttggcg gggaactcct
     5521 gcggcccgtg aacaacctgg tttccctcta taacgcggtc tccctcacgc acctccttcc
     5581 cgtgggggga gaggatcggg accgcctttc cggcgacgtc cggctgaccc gggcgggtcc
     5641 cgccgaaccc ccggtgatct tgttaggaga gaaggatccc cgatcccccc acgagggaga
     5701 agtgatctac accgacgaca tcggcgccct atgtcggcgc tggaactgga aggaggcgga
     5761 ccggaccaag ctgacgccgg agacgaaaaa cgtcattttg gtcatcgaat ccctaccccc
     5821 cctccccccc gacgcgctgc cgaaggcggc ggacgacctg gcggcgctgg tggagcgcca
     5881 cctggggggc acggtgtcca cgctccttct ccaccgggga aatcccgagg ggatcctccg
     5941 ctagggccgg gtaaaagcgc cccccttccc tagggaaggg gggcgctttt accccctcga
     6001 cgagccccgg gtgtcccacc gggagcggag atcttcccat tggcgccggg cgacggggtc
     6061 gtcttcccct tctcccaaga gggaggcgac gcgggcaaag accttttcca tgacctcctc
     6121 gcgcgtggct tccttgtggc ggtacatggc ctggtccgcc tcgcgaaaga ggcgctccat
     6181 ggagggcttc cccccctcct ggtggtgggc gagccccagg gagagcccca gatagagcgg
     6241 ggagctggcg ttccactcct ccacgtggcg ggcgagatcg cggcatcggc tctgggccac
     6301 ggggccgggg cactggggga ggaggatggc gaactcgtct cccccgatgc gggccaccac
     6361 gtcgctggat cgaaaggtgc ggcgcagcac ctccgccgcg ccccggatca aggtgtcccc
     6421 ggcggcgtgc cccgccacgt tgttcacgat cctgagccca tccacgtcgc agaccaaaag
     6481 ccccacgggg ttgaagcgcc ccgtggctag gcgtcgcatc tcctcttcga aatatccccg
     6541 gttatgcagc cccgtgaggg cgtcgtgaaa gctcgcgaag cgcaacgcct cctccgcccg
     6601 gcgccgctcc gtcacgtcct ggacaatggc cagcaggtgg tcggaggggt cgtccggaga
     6661 ggggggcagt agggtcttgg tgaaggtgaa gagacggctc tcccggtggg gggtgagcac
     6721 cggcacctcg ggaaaggtgt gggcctggga ggattgggcc acctcccggt cctgggcggc
     6781 gaagatctcc cccggggtga ggccgaagat ctcctcgtcc gtgcgcccct ccaggtggtc
     6841 ccgggggagg ccgaagaggg tctccaccgc ccggttgagc atggtgtaac gccccgagga
     6901 gaggtctttc accaagatgg cctcggggac gtgctccagc acggcgctga tgaaccgccg
     6961 ctgcctctcg atgtcccgct ccacccggtg ccgctcctcc acttcccggc ggaggagctc
     7021 cttttcctct tccaggtgcc gggtggcccg ccgcacccgc caccccagga tcatcatccc
     7081 caggaccccc actcccccca agaaggcggc ccccagtgcc gtgtagagga gctggcgcct
     7141 cagggcggcg ttctggggaa gggcccgggt ccagcgctgc tgcaactgga gcaaggtgcc
     7201 atcctgcttc aaaatggtga gggccttgtt cacggccgcc gccatggccc ggtcgtcgga
     7261 gcgcatcgcc atggcgtagg ggctcgaaag gaggggagcc tccagggcgc gaaagaggtc
     7321 ggtgaggtgg tcccgggcta tggtgtagta caccacggct tcgttgccca cgaaggcgtc
     7381 cacctcgcca tccgccaggg ctcggagggc ctggggctgg tcgtcgtaga gccagaggtc
     7441 ggattctgac aggtactggc ggcaaaagtc ctccgccaga tcccgccgct gcacccccac
     7501 ccggcgcccc cagaggtcgg cggaggtgtt gatgtgggta gcggcgacgc gggtgaaggc
     7561 cgtcaactta ttgacgcaat gggggaaggc gaagcggtag cggccctccc gctcgggggt
     7621 gacccgcatg gcggcgagga tgtccgccga tcctatctcc agggcgagga ccgcctcggc
     7681 ccaggggaga aggtcgatgg agacctcttc ccccatccgc tccccgacga gacgggccac
     7741 ggcggcatcc ataccggagg gctcccctcg ctcgtcgagg aacatgaagg ggggaaagtt
     7801 ccggtccccc accacgcgca acaccccctc cgccggaagg gatagggcca cgacaaaaaa
     7861 aatcagtaag gataacgccc gggggcccac gatggatcat ctcgcttttg tttggtcgat
     7921 ttcccgcttc ctttcccgcg gcgctgcccc atcatagcac gaaaaagggg agtgcgggaa
     7981 catggtgtta gcgggagttc ccccctatca tggcatggcc tgggggcgct cctccggggg
     8041 acttcggacg cccccggagg gatcgagcgc tccgactttt ccccggttat ggcgggaaaa
     8101 tcctcctcgc tcgaggacaa gggaggcgcg gggcgtatgc gatatgcacg atctcccttc
     8161 gattgccata acgtcccttc cctgtataat gacccccatg aagcccgggg cctcgcgggc
     8221 tggatgtcgt gttttgtatg gaggtgaccc atgcgtgccc acgttttcaa agcctgttta
     8281 gccctctgca tggtgctgat catgggggcg gcgctccagg agcgctttcc gattcaccgg
     8341 ccgacgacca tatttcatgg aggggggcgg atctatgccc ccatggtgat ccggatgatg
     8401 agccccgccg tggatgcgga gctccgggcc atgggggcga gccgacgtca cctgctggag
     8461 tggcgcgatc gccatcccac cgccgccctc cgccacctgt ggagcatgga ggaggaggat
     8521 cagaggacct gcgcccgcat cgcccgctac atcctgagac aaaaccgggc catcacgccg
     8581 gaactggcat gggtggaggc ggcggcgctg gtgcactaca gcgccaagta cggcgccccg
     8641 gtggagctcg ccgtggcggt ggccaatacg gagagccatt tcagcccgga gtccctttcc
     8701 agctacggcg ccatgggggt gatgcaggtg gtgtggcgta tccaccacgg tttgctccag
     8761 gccaacggca tcctttcagg agaagagctc cacaaccccg atcgggggat cgcggcgggg
     8821 tgcctcctgc tggggcgcta ccttcgggcc gaaggagaca cccgggaggc cctggggcgc
     8881 tacgccggag ggtcggtgga ggcctattgc ggcaaggtgg aggggtacgt ggaccgcttt
     8941 cgccgcctcg tggcggaggg gggaacctcc cggggcatgt agctttcgtc cctatgcccc
     9001 ggcgaaaggg gccttttgcg ccccctcggg gggctccggc ctgagcgctt gagtcggggg
     9061 gcgaggcgcg tcctgagagt ttgaaatatg gcgtgcattg acgcgacctt gccgtggcgt
     9121 tatgctgtgt tgacgaccct ggagaggtcc cccgccccgg cggggggctt gttgctgttt
     9181 gctccgatga ggggcgccga actactcgga aaggggcgcg ggacccgagg cggagggcgc
     9241 ccttttcagg aagggagggg agaccatggc caaaaagata gcccccaccc gggggaacct
     9301 gttgcgggtc ctgcgggcgc tgcacctggc gcgcaagggg cacgatctgt tggagcagaa
     9361 gcgccaggtg ctcatgatgg agctgatgcg ccacattgac cgggcccggg acatccagaa
     9421 ggacatggcg gagatctttg ccgctgccta ccgcgccctg cagcacgcca acgtctccgt
     9481 gggcatcgac acggtccagg agatctcccg ggcggtgccc gaatcggacg agttcatcgt
     9541 ccggttgcgg tcggtgatgg gggtggatat ccccgaggtg gatcccatcc ccgaggcctg
     9601 tccccccggc tactcctttc agggtacgtc gggggcggtg gaccgggcct ttctggcgtt
     9661 ccatcgggtg cgggtgctgg tggcccggct ggcggaggtg gagaccagcg tctaccgtct
     9721 ggcggtgcag atccgcaaga cccaccgccg ggtgaatgcc ctggaaaagg tggtgatccc
     9781 ccagtatggc gccgccgccg tggccatcgg ggcggtcctc gaagaggggg atcgggagga
     9841 cttcgcccgg atgaagatct ctaaagaggc ggggaaaaag ggcctcggcc gtgaggttcc
     9901 ctagggcaag gttttgaggc gggcgatcac ccccgggcag accccgggga aaagggggga
     9961 gaggggccgt ggcggcattg caggaagtgc tggcggtgtt gttgggcgcc gagggcgagg
    10021 cgcggtcgga gatagaagag tcccgaaacg aggcggagcg ggtggtgcgc cgggccaaag
    10081 acgccttctc tcaggagagg gagcgtcgcc tggcggcggc ccgagagcag gcgaaggccg
    10141 tcttggactt ggctcggtcc gccgccgacg ccgagggggg acagatcttg cagatgggcc
    10201 agcaggacct cgaccggatg cgggagcgct ttgacgaaaa cgtccaggga ttggtggcca
    10261 cc
//
LOCUS       DAOOER010000062        10170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_7089_length_10170_cov_7.958972, whole genome shotgun sequence.
ACCESSION   DAOOER010000062 DAOOER010000000
VERSION     DAOOER010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 10170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10170
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7089_length_10170_cov_7.958972"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="PL162_07740"
     CDS             complement(<1..1193)
                     /locus_tag="PL162_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(111..113),aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQF91944.1"
                     /translation="MQSIKAILDGREITARPGQTILQAARANGMYIPALCDHPALSPF
                     GACRVCLVEVEKNPKLLPACTTPLTEGMVIASRSPKVLEARRAVVELILVRHPLDCFS
                     CPSNGKCELQNVAYELGITSSRFREDVDVQKGVHRIEDANPFYVRDMDKCILCGRCVR
                     ACDELARYHAIDFQQRGVKTMVHPPIGRDLENSDCVFCGQCVQLCPVGALYEKSSVGQ
                     GRPWELRQERTTCAYCGVGCELIIHVNKHTGRIANITTDYESKTALNQGRCCVKGRFA
                     WQFVHSEDRLTKPLVRENGVFREASWDEALGLVAYKIGHIKEHHGPDSLGFFSSARCT
                     NEENYLFQRLAREVVGTHNVDHCAHLUHSATVAGLGETFGSGAMTNDFASIKEADVML
                     VIGSNT"
     gene            complement(1213..3012)
                     /gene="nuoF"
                     /locus_tag="PL162_07745"
     CDS             complement(1213..3012)
                     /gene="nuoF"
                     /locus_tag="PL162_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HQF91945.1"
                     /translation="MAEYRARGHVLVCGGTGCTASGGKVLRESLTKELAARGLDQEIK
                     VVETGCHGMCEMGPIVVVYPEGTFYCRVVPEDVKELVEEHLLKGRVVSRLLYRTPMSP
                     QGVPHYKEIPFYSKQRRIALANCGYINPKKIEEYIARDGYTALAKVLTEMTPEEALQE
                     LKTSGLRGRGGGGFPTGLKWEFCRKSPGDKKYVICNADEGDPGAFMDRSLLEGDPHAI
                     IEGMMIGAYVMGSDEGYIYCRAEYPLAIKHLQVAMEQAEELGLLGDHVMGTDFSFHLH
                     VKEGAGAFVCGEETALMASIEGTRGMPRPRPPFPANKGLWGKPSNINNVETWANVPAI
                     IRHGGAWYASIGTEKSKGTKVFALTGKVKNTGLIEVPMGISIREIVFELGGGIIDDKK
                     FKAVQIGGPSGGCLTEVHLDTPVDYESLTAAGAIMGSGGLVVMDEGTCMVDVAKFFLE
                     FTQRESCGKCVPCREGTKKMLDILTRITEGNGKPEDIDTLQYLGSQIKNASLCGLGQT
                     APNPVLTTLRYFRHEYEAHIFDKKCPAGACKSLLQFIIDGDKCIGCTKCARVCPACAI
                     SGEVKKPHLIDQAKCIKCGACEAACPVKAISKR"
     gene            complement(3038..3409)
                     /locus_tag="PL162_07750"
     CDS             complement(3038..3409)
                     /locus_tag="PL162_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HQF91946.1"
                     /translation="MPKITSLDDLRRIKEASSDLTAARSQGRTRVIVGMGTCGIAAGA
                     RDVMQALMVELEKRDLRDVSVETTGCIGMCQQEPLVDIIRPGEPRITYGRLTASDIPR
                     IVAEHLVNGNIVAEKVIGRTE"
     gene            complement(3471..3956)
                     /gene="nuoE"
                     /locus_tag="PL162_07755"
     CDS             complement(3471..3956)
                     /gene="nuoE"
                     /locus_tag="PL162_07755"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HQF91947.1"
                     /translation="MGDQVLAQETTAVDEGVRQLVESFRGKKGFLIPLLQGIQHHCGY
                     LPAEVLEYVAEELRVSLAEIYGVCTFYAQFHLNPRGRHIIRVCRGTACHVRGSLAIME
                     RLKSELHVAENGTTEDLRFTLEPVACLGACGLAPAVTIDDDTHGRLTPDGAAKILERY
                     P"
     gene            4237..5553
                     /locus_tag="PL162_07760"
     CDS             4237..5553
                     /locus_tag="PL162_07760"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HQF91948.1"
                     /translation="MGEGKVPNRWVVAIAGTVLQLCLGTVYAWSFFQKPVVTTFGWSN
                     SQAAWAFSFAICFLGLAAAWGGINLAKHGPKKLALTGSVLFSSGYLLGALAFSMKSLI
                     MLYVGYGMVGGIGLGLCYVTPVATAAKWFPDKKGFITGMVVMGFGFGALLMSKIIAPI
                     FMEMTGENLVSVFLYIGILMFVLTIPTASLLVNPPAGFVPAGYTPPAAGASAQKGGSA
                     GEISPREALSSPKFMMMWLVFFLNITAGIMFIGFQSPMMQDLLKSADPSYGSMAPEAA
                     AKALAAAGATLIAISSVFNGVGRFFWGGVSDKIGRTNAFRLILLTQACVFAALLFVKT
                     PLIFGALVCYVLLCYGGGFGTMPSFVLDVFGNKLMSTVYGVILTAWSLAGIVGPQVVA
                     TITDVAPASAASYTFIIGGGLLAVGFVVALFLRDSAFARPEGAGAK"
     gene            complement(5709..6077)
                     /locus_tag="PL162_07765"
     CDS             complement(5709..6077)
                     /locus_tag="PL162_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91949.1"
                     /translation="MGSQDVLKIYVEGPGFDVGCCPVHYGQEEMATFPGAQQRRQVGA
                     LLVRLRERFGDGLRVDLIDPRSLMALGALFRFRLKGTEVAWVWRKKLIFRGIPAWEEL
                     EKALIEEGVVIPRIPPQGSR"
     gene            complement(6094..7116)
                     /locus_tag="PL162_07770"
     CDS             complement(6094..7116)
                     /locus_tag="PL162_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HQF91950.1"
                     /translation="MKVREVSLEEAVGLPLSHDLTQVWPETKFKGPRFSRGHVVTRED
                     FAVLRDMGREHLSVLDLDPSEVHEDDAALALALSLAGSGVEIRGPREGKCSLLATHRG
                     LLRFDPDTIHALNEDLLWSVVTLPPRMAVEAGQEVAACRIMPLAMDKDRVDRACDRSR
                     PLEVLPFRPLGVGLVTTGRELAEGRIPDAFMPKLEKKLYRYGGHVVAQRYATDDPDRI
                     VGAIEELIALGATMVLCTGGMSVDADDRTPGALRRAGDRVCFQGVPMMPGNMLMLALR
                     GDIPLVGAPACVAYDERTSLDPLMDLLAAGIYPTAEEVRRWGVGGLCLRCPSCAWPRC
                     SFGSGA"
     gene            complement(7113..8216)
                     /locus_tag="PL162_07775"
     CDS             complement(7113..8216)
                     /locus_tag="PL162_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQF91951.1"
                     /translation="MTPWGEVALTHRRGSFQLRASFSFDREIGVLFGPSGAGKSSLLR
                     LLAGLERPEEGRFCLGDRVLVDCARRIFTPPHRRRVGMVFQHLALFPHRTVEENVAHG
                     VRDQDTLQARETARQWLRRVHLEGFEGRYPSQLSGGQSQRVALARSLAASPELLLLDE
                     PFSALDGPLRRSLRRELRRLHEETKIPVIYVTHAIEDACALGHRVFFLQDGACTGSVK
                     VEHLWNGSAQGEVFEALRWGNLLSGEFVLRDHKRFFSWDGGMLEVALPAEGCPGPGRV
                     FIAPHDVKVLYEGVVVDEDLRANVVCGIVGENLTLGSLCRLSVETSSGVWQVEYPSGA
                     YDTMDLKEGCAVRLAIRPGSVVPLGDGRKERER"
     gene            complement(8200..8844)
                     /gene="modB"
                     /locus_tag="PL162_07780"
     CDS             complement(8200..8844)
                     /gene="modB"
                     /locus_tag="PL162_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HQF91952.1"
                     /translation="MDEALRLSLEVLAVDVPLMILLGVPLGWLLARKAFPGRKVLELF
                     VLIPVVLPPSVLGLYLLLALGQLPGVRAWRLLFSFPALILGALIPALPLVVQGARAGF
                     SSVSRDLEDAARTLGDSEWRVFFRITLPLARRSLGAGLALGTARALGEFGVSLMIAGN
                     IPGVTQTLPLYIYSRAEALDFAAAHGAALLLAAVGVATLIFVRHLEEGRHDPVG"
     gene            complement(8851..9717)
                     /locus_tag="PL162_07785"
     CDS             complement(8851..9717)
                     /locus_tag="PL162_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HQF91953.1"
                     /translation="MGHILDLIHEEISQGKKGVLCTLVEVVGSAPRHLGARMWVRPDG
                     SAVGTIGGGTVEFRIQERARAMLRRREGPGLVRESMWSDEVSEGEPACGGEVGIFLEI
                     LGQDRELIIFGAGHVGLAVARLGAFVGFAVTVWDERPEFASPERFPEARVLACPVDRV
                     FEEGLRLSEESYVVIATRGHALDAAVAKALDGLPAAYIGMIGSRKKIATVREGLRRDG
                     VSQRHLDRLFQPVGLPIGAETPEEIAVSVLSEIIAVASGADIGGLREPLRRGGSPAFP
                     LFSGGAPDPEGV"
     gene            complement(9778..>10170)
                     /locus_tag="PL162_07790"
     CDS             complement(9778..>10170)
                     /locus_tag="PL162_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQF91954.1"
                     /translation="ACTGRMDDLPLLDASALAPGVLKRIVFGPGRFWSDWVMRYFILP
                     PGEKVPAHSHDWEHLAVALQGVGRLVIDGVAEDFSQGRWGHVPAGRPHSFENGGSEPF
                     AFLCIVPTSGDPHAKRAQHRLSRQKSDE"
BASE COUNT         1920 a         3237 c         3067 g         1946 t
ORIGIN      
        1 gtgttggatc cgatgacgag catcacgtcg gcttctttga tggacgcgaa gtcgttggtc
       61 atggcgccgc tgccgaaggt ctcaccgaga cctgccacgg tggcgctgtg tcaaagatgg
      121 gcgcagtggt ctacgttgtg ggtacccacc acctcccgag ccaggcgttg gaagagatag
      181 ttctcttcgt tggtgcatcg ggcggaggag aaaaatccca gagagtcggg accgtggtgc
      241 tccttgatgt ggccgatctt atacgccaca agccccaggg cctcatccca ggatgcttcc
      301 cggaagacgc cattttccct taccagtggt ttcgtcaagc ggtcctccga atggacaaat
      361 tgccaggcaa agcgtccctt tacgcagcag cgtccttggt tgagcgccgt cttgctttcg
      421 tagtccgtgg tgatgttggc gatgcggccg gtgtgcttgt tgacatggat aatcagctcg
      481 caccccacgc cgcagtaggc gcaggtggtc ctttcctgac gcaactccca aggacgcccc
      541 tgccccacgg agctcttctc gtaaagggcc cccacgggac aaagctgaac gcactggccg
      601 caaaagacgc agtcggagtt ttccagatcc cggcctatgg gggggtggac catggtcttg
      661 acgccgcgct gctgaaagtc gatggcgtga taacgggcca gctcgtcgca ggctcggacg
      721 caccttccgc agaggatgca cttgtccatg tcccggacat agaagggatt ggcgtcttcg
      781 atccggtgta cgcccttttg gacgtccaca tcttcgcgga aacgggacga tgtgatgccc
      841 agctcatagg ccacgttctg gagctcgcac ttgccgttgc tggggcagga aaagcagtcc
      901 agggggtgac gcaccaggat aagctccacc acagcccgac gagcctctaa aaccttggga
      961 ctccgagagg cgatgaccat accctccgtc agaggcgtcg tgcaggcggg gaggagcttg
     1021 ggattttttt ccacctccac caggcacacc cggcaggccc cgaagggact gagggccggg
     1081 tggtcgcaga gggcgggaat gtacataccg ttcgcccggg cggcctgaag gatggtctgg
     1141 ccgggccggg cggtgatttc ccggccgtca agaatggctt tgatgctctg catgatatga
     1201 gaccctcccg ttctagcgct tggagatggc cttaaccgga caggccgctt cacaggcacc
     1261 gcacttgatg cacttggcct ggtcgatgag gtgaggtttt tttacctctc cggaaatggc
     1321 gcacgccgga cagacccggg cgcacttggt gcagccgatg cacttgtcgc catcgatgat
     1381 gaactgcaaa aggctcttgc aggccccggc gggacacttt ttgtcgaaga tgtgggcctc
     1441 gtactcgtgc cggaaataac gcagagtggt cagcacgggg ttgggagccg tctgccccag
     1501 gccgcagagg gaggcgtttt tgatctgact tcccagatac tgaagggtat cgatatcttc
     1561 gggcttgcca ttaccctccg tgatgcgcgt gaggatgtcc aacatcttct tggtgccctc
     1621 ccggcagggc acgcacttgc cgcaggattc tcgttgggtg aattccaaga agaacttggc
     1681 cacgtccacc atgcaggtgc cctcatccat gaccaccagt ccccccgacc ccatgatggc
     1741 tccggcggcg gttagggact cgtagtccac gggggtgtcc aaatggacct cggtcagaca
     1801 tccccccgag gggccgccaa tctgcacggc cttgaatttt ttatcgtcga taatgcctcc
     1861 tccgagctca aaaacgatct cccggatgga aatccccatg gggacttcga tgagccccgt
     1921 gtttttcacc ttacccgtga gggcgaaaac cttggtgcct ttggacttct ccgtgccgat
     1981 ggaggcatac caggcgccac cgtggcggat gatggccgga acgttggccc aggtctccac
     2041 gttgttaatg ttggagggct ttccccagag acccttgttg gcgggaaagg gagggcgggg
     2101 gcggggcatg ccccgcgtac cctcgatgga agccatgaga gcggtctctt cgccgcacac
     2161 gaaggccccc gccccttctt tgacgtgcag gtggaaggag aagtccgtcc ccatgacatg
     2221 atctcccagc aggcccaact cttccgcctg ttccatggcc acttgcaggt gcttgatggc
     2281 caggggatac tcggcccgac agtagatata tccctcgtcg gatcccatga cataggcgcc
     2341 gatcatcatg ccttcgatga tggcgtgggg gtccccttcc agcagggacc ggtccatgaa
     2401 ggccccgggg tctccttcgt cggcgttgca tatgacgtat ttcttgtccc cgggggactt
     2461 ccggcaaaac tcccacttga gcccggtggg gaagcctccg ccgccccggc cccggaggcc
     2521 cgaggtcttc aattcttgga gggcctcttc gggggtcatt tccgtgagga ctttggccag
     2581 agcggtgtat ccgtcccggg caatgtattc ttcgatcttt ttagggttga tatagccgca
     2641 attcgccagg gcgatgcggc gctgcttgct atagaaggga atttctttgt agtggggtac
     2701 cccctggggg ctcatggggg tgcggtagag cagacgcgac acgacgcgcc ccttgagcag
     2761 atgctcttcc accagttctt tgacgtcttc cggaaccacc cggcagtaga aggtcccctc
     2821 ggggtaaact accacgatgg gtcccatctc gcacatgccg tggcatcccg tctccaccac
     2881 ttttatctcc tgatccagcc cccgagccgc cagctcttta gtcaggctct cccggaggac
     2941 cttgcctccg cttgccgtac agccggtgcc gccgcaaacc agcacatgtc cccttgcgcg
     3001 gtattccgcc acagttcact cctccttgaa aaaaaaccta ttcggtgcga ccgatgactt
     3061 tttctgccac gatgttgccg tttaccaggt gttcggccac gatgcggggg atgtccgagg
     3121 ccgtaaggcg cccgtaggta atgcggggct ctccggggcg gatgatgtcc accagaggct
     3181 cctgctggca catgccgatg caccccgtgg tttccaccga cacgtcgcgc aaatcccttt
     3241 tttccagctc taccatgagg gcttgcatca cgtctcgagc tccggcggcg atgccacagg
     3301 tccccatgcc cacgatgaca cgggtgcgcc cctgggatcg ggcggcggtg aggtcggacg
     3361 aggcttcttt gatccttctc aaatcgtcaa ggctggtaat tttgggcatt tttatcctct
     3421 ccataatttt ttttggccca tgggccttcg gagttcttgt cggaaagcgc ctacgggtag
     3481 cgttccagga tcttcgccgc gccgtcaggg gtgagacggc cgtgggtatc gtcatcgatc
     3541 gttaccgccg gagcaaggcc gcaggccccg aggcacgcca caggttccag ggtaaaccgg
     3601 agatcttccg tggtgccgtt ttccgccacg tgcaattcgc ttttgagcct ctccatgatg
     3661 gccagacttc ctcggacatg gcaggcggtg ccacgacaga ctcggatgat atgccgccct
     3721 cgggggttaa ggtgaaactg ggcatagaac gtgcacactc cgtagatctc cgccagagat
     3781 acccgcaatt cctccgccac gtactccagc acttcggcgg ggaggtaacc gcagtggtgc
     3841 tgaataccct ggagaagggg aatgagaaag cccttcttcc ctcggaagga ctccaccaat
     3901 tgccggactc cttcgtccac cgccgtggtt tcctgtgcca aaacctgatc tcccatcatt
     3961 tcggacagcc tgcgccgtcc ccacctccct aaaatgtttt ttcgcgctcc ccgacttcag
     4021 aaaaaaacta acccccctca gaatgtctga aaaaggggta aagggggcaa aaaaaatcaa
     4081 aaacgttgac agctccattg acaatatatc cgttagcgat tataccatgt cttgttccca
     4141 aatgcacaag atgggggcga gaaagttttt ttatccacac ccttggcggc cccggggggg
     4201 cgggggggaa aataatttct tggagggatg ggaaaaatgg gagagggcaa ggttccaaat
     4261 cgttgggttg tggccatcgc gggaacggtg ttgcagttgt gtctcggcac ggtttacgct
     4321 tggagtttct ttcagaaacc cgtggtgacc acctttggct ggagcaacag ccaagccgct
     4381 tgggcgttca gcttcgccat ctgctttttg gggttggccg ccgcttgggg gggaataaac
     4441 ctagccaagc atggtcctaa aaagttagcc ctgacgggga gcgtgctctt ttcctcggga
     4501 taccttctag gagccctggc cttttccatg aagagcctga tcatgctcta cgtgggatac
     4561 ggcatggtgg gtggtatcgg gctggggctg tgctacgtca ccccggtggc cacggcggcc
     4621 aagtggttcc cggacaaaaa aggattcatc accggcatgg ttgtcatggg gttcggtttt
     4681 ggcgccctgc tcatgtccaa gatcatcgcc ccgatcttca tggagatgac gggggagaac
     4741 ctggtgtccg tgttcctgta catcggcatc ctcatgttcg tcctcacgat ccccacggcg
     4801 tcgctgctag taaacccccc cgctggcttt gtcccggcgg gatacacgcc cccggcggcg
     4861 ggagcctcgg cccaaaaggg agggagcgcg ggggaaattt ctcctcggga ggccctttct
     4921 tctcctaaat tcatgatgat gtggctggtg ttttttctta atatcacggc gggaatcatg
     4981 ttcattggct tccagtcccc catgatgcag gatcttctga agagcgccga cccttcctat
     5041 ggatccatgg ccccggaggc ggcggccaag gccttggcgg cggcgggggc cactctcatc
     5101 gccatcagtt cggtttttaa cggtgtgggg cggttctttt gggggggagt ttcggataaa
     5161 atcgggcgga ccaatgcctt ccgcctgatc ctcctgaccc aggcctgcgt ctttgccgcg
     5221 ctcctcttcg tcaagacccc cctgatcttc ggcgccctag tctgctatgt gctgctgtgc
     5281 tacggcggag ggttcggcac catgccgtct tttgtactgg acgtcttcgg aaacaagctc
     5341 atgtccacgg tttacggcgt catcctcacg gcctggtcct tggcaggcat cgtggggccc
     5401 caggtggtgg ctaccatcac agacgtggcc cccgccagtg ccgcatccta cacgttcatc
     5461 atcggcggag ggctcttggc ggtaggcttt gtcgtggccc tcttcctccg agacagcgct
     5521 tttgcccgac ccgagggcgc tggcgccaag taggaccgcc tccgggggat tttaccgtag
     5581 acattagggc tccggaggga tagcccgtga tccccccgga gccccatcag cgcgagtccc
     5641 ccggaagagc ctcggttctc cggggggttt gctttttccc gtccgctaag ggacttcggc
     5701 ccacaaagtc accttgaacc ctgcggtggg atcctcggga tgacgacgcc ttcttcaatc
     5761 agagcctttt ccagctcttc ccaggctggg atgcccctga agatgagctt ctttcgccag
     5821 acccaggcca cttctgtgcc ctttaggcga aagcgaaaaa gagctcccag ggccatgaga
     5881 ctacgggggt cgatgagatc gacccgcagc ccatcgccga aacgttccct aaggcgcacc
     5941 aacagcgccc ccacctggcg gcgctgttgg gctccgggaa aagtggccat ttcctcctgg
     6001 ccatagtgta cggggcagca tcccacgtcg aagcccgggc cttctacgta tatttttagc
     6061 acgtcttgcg atcccatcgg aaggcacatc ccctcacgcg ccggagccaa aagagcaccg
     6121 gggccaggcg cacgacggac atcggaggca tagccccccc acgccccagc gccggacctc
     6181 ttcggcggtg gggtagatcc ccgccgcgag gaggtccatc aggggatcca aagacgtcct
     6241 ttcgtcgtac gccacgcagg cgggagcccc caccaggggg atgtcccctc ggagggccag
     6301 catcagcatg ttcccaggca tcatgggaac gccctggaag cacacccgat cccccgccct
     6361 tcgcagggcc ccaggggtcc ggtcatcggc gtcgacgctc atgcctccgg tgcagagcac
     6421 catggtggct cccagggcaa tgagctcttc tatggcgccc acaatccggt cggggtcatc
     6481 ggtggcgtac cgctgggcca ccacgtgccc cccgtagcga tacagctttt tctcaagctt
     6541 cggcatgaag gcatccggaa tccggccttc ggcgagctct cgccccgtgg tcaccaaccc
     6601 cacccccaag gggcgaaagg ggaggacttc caggggacga gatcggtcgc aggcccggtc
     6661 aacccgatct ttatccatgg ccaagggcat gatacggcag gccgccactt cctgtcccgc
     6721 ttccacggcc atgcgagggg ggagggtgac gacgctccac aggaggtctt cgttcaaggc
     6781 atgaatggtg tcggggtcga agcgtagcaa cccccgatgg gtcgccaaga gggagcattt
     6841 gccctcccgg ggaccccgga tttccacgcc ggagcccgcc agagacaagg ccagggcgag
     6901 cgccgcgtcg tcttcgtgga cctccgaagg atcgagatcc agcacagaga ggtgttccct
     6961 gcccatgtcc ctcaggacgg cgaaatcctc ccgggttacc acgtgccccc gggaaaaacg
     7021 gggccccttg aacttcgtct ccggccacac ttgagtcaag tcatgggaga ggggtagtcc
     7081 cacggcctcc tcgagagata cctcccgcac cttcatcgct ctcgctcctt ccttccatcg
     7141 cccaggggca ccacgcttcc ggggcggatc gccaggcgaa cggcacatcc ttctttcaag
     7201 tccatggtgt cgtaagcccc cgagggatac tccacctgcc acacccccga ggaggtctcc
     7261 accgaaaggc gacataggct tcccagggtg agattttccc ccacgatgcc gcagaccacg
     7321 ttggccctca ggtcttcgtc gacgacgaca ccctcgtaga gcacttttac atcgtggggg
     7381 gcgatgaaga cccttccggg ccccggacat ccttctgctg gtagcgccac ttccaacatc
     7441 ccgccatccc aagaaaaaaa tcttttgtga tcccgaagca cgaactctcc ggagaggagg
     7501 ttgccccaac ggagggcctc gaagacttcc ccttgggcgc tcccgttcca gagatgttcc
     7561 accttcacgc tgcccgtgca ggccccgtcc tggagaaaaa aaacccgatg tcccagggcg
     7621 caggcgtcct ctatggcgtg agtcacgtag atcacgggga tctttgtctc ttcgtgaagg
     7681 cgccgaagct cccgcctgag gctccggcgc agggggccgt ccagggcgct gaagggctcg
     7741 tccaaaagga gtagctcggg ggaggccgcc aaggatcggg ccagggccac ccgttggctc
     7801 tggcctccgg agagctggga ggggtagcgg ccttcgaagc cctccagatg gacccgtcgg
     7861 agccactggc gcgccgtctc ccgggcctgg agggtatcct ggtctcgcac gccgtgggcc
     7921 acgttttctt ccaccgtacg gtggggaaag agggccaggt gctgaaatac catgcccacc
     7981 cggcgccggt ggggcggggt gaagatccgg cgggcgcagt ccaccaacac ccgatccccc
     8041 aggcaaaatc gcccctcttc ggggcgctcc aaccccgcga ggaggcgcaa aagggagctt
     8101 tttcccgcac cgctggggcc gaagaggacg ccgatttctc ggtcgaagga aaaagaggcc
     8161 cgaagttgaa aggatccccg acggtgggtc agggccactt caccccacgg ggtcatggcg
     8221 cccctcctcg aggtgtcgca cgaaaatcag ggtggccacc cccaccgccg ccagaaggag
     8281 ggcggcgccg tgggctgcgg cgaagtccaa ggcttcggcc cggctataga tataaagcgg
     8341 caaggtctgg gttaccccgg gaatgttgcc cgcgatcatg agggagaccc caaactcccc
     8401 tagggcacgg gcggtgccca gggccagacc cgctcctagg ctgcgacggg ccaggggcag
     8461 ggtgatccga aaaaaaacgc gccactccga gtctcccaac gtgcgagccg catcttccaa
     8521 atctcgagag acggaggaaa aacccgcccg ggctccctgg accaccaagg gaagggcggg
     8581 aatcagggct cccaagatca gggcggggaa ggaaaagagc aggcgccacg cccggacgcc
     8641 ggggagctgg cccagggcca aaaggagata gagccccaac accgaggggg ggagcaccac
     8701 ggggatcagg acaaaaagct ccaggacctt ccggcccgga aaggccttcc gggccaagag
     8761 ccaccccagg ggcaccccca aaagaatcat cagggggacg tccaccgcca ggacttccag
     8821 gctgagccgc agcgcctcgt ccatggggcc ctagacgccc tcggggtccg gagcccctcc
     8881 ggaaaagagg ggaaaggcgg gcgatccccc ccggcgaagg ggttctcgca gccccccgat
     8941 atccgctccg gaggccacgg cgatgatttc tgatagcacc gaaacggcga tctcttccgg
     9001 ggtttccgcg ccaatgggca gtcccacggg ctgaaagagg cgatccaagt gcctttggga
     9061 cacgccgtcc cgccgcaacc cctctcgcac ggtggcaatc ttcttgcgag aaccgatcat
     9121 accgatatag gccgctggca ggccgtcgag ggccttggcc acggcggcgt ctagggcatg
     9181 gccccgggtg gcgatgacca cgtagctctc ctcggagaga cggagcccct cttcgaaaac
     9241 tcgatccacg ggacacgcca acacgcgggc ttcgggaaag cgctccggag aggcaaattc
     9301 cggccgttcg tcccagacgg tgaccgcaaa ccccacgaag gcccccaacc gggccaccgc
     9361 cagccccacg tgtcccgcgc cgaaaatgat cagctcccga tcctgcccca ggatttccag
     9421 gaagattccc acctcgccgc cgcaggcggg ctctccttca ctcacttcgt cgctccacat
     9481 ggattcccgg accagtccgg ggccctctcg ccgccgcagc atggctcgag ctcgttcctg
     9541 aatgcgaaac tccaccgtgc cgcccccgat ggtcccgacg gcgctaccgt cggggcggac
     9601 ccacatgcgg gcccccagat ggcgaggggc ggaacccacg acctccacca gggtgcagag
     9661 tactcccttt ttcccctggg aaatctcttc atggatcaaa tctaaaatat gccccaccac
     9721 gtcacccccc tctgcgatcg aaatggttac cccccaaaag acatcgcccc ggggggctca
     9781 ctcgtccgat ttttgtcgtg atagacgatg ctgggcgcgc ttggcgtgag ggtcccccga
     9841 ggtggggacg atgcacaaaa acgcaaaggg ctccgatccc ccgttctcga aggagtgggg
     9901 gcgccccgca ggcacatggc cccatctccc ctgggaaaag tcctccgcca cgccgtcgat
     9961 gaccaaacgc ccgacccctt gtaacgccac ggccaggtgc tcccagtcgt gggagtgcgc
    10021 gggaaccttc tctcccggag ggaggataaa atagcgcatg acccagtcgg accagaatcg
    10081 ccccgggccg aaaacgatgc gcttgagaac cccgggggcc agggcggagg catccagcag
    10141 gggaagatcg tccatcctcc ccgtacacgc
//
LOCUS       DAOOER010000063        10068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_7177_length_10068_cov_8.049036, whole genome shotgun sequence.
ACCESSION   DAOOER010000063 DAOOER010000000
VERSION     DAOOER010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 10068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10068
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7177_length_10068_cov_8.049036"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PL162_07795"
     CDS             complement(<1..293)
                     /locus_tag="PL162_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HQF91955.1"
                     /translation="MGDRGTGRRGYFGSFGGQFVPETLMGPLEELERAFDRYGNDGDF
                     RRDLGDLLRDFSGRPTPIYEAHRLSAQRGVRVFLKREDLNHTGAHKINNALGQV"
     gene            complement(286..951)
                     /locus_tag="PL162_07800"
     CDS             complement(286..951)
                     /locus_tag="PL162_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HQF91956.1"
                     /translation="MMPLPLRIKMCGLTREGDVAAAVRQGVDALGFILAESPRRVSLE
                     RVRQLTVGLPPFTTVIGVVVNPTDGELRELVASRLFDGVQFSGDEPPELLRDFPLRTI
                     KAFGIRDAADVEAALRYEGADVALFDAGNPGARGGTGRPFDWGLLHRRPRGPFLLAGG
                     LGPENVAEAVRAVRPWGVDFNSQVERSPGEKDEGLVRRAVEAARRAGSEVASEEEGSG
                     GHG"
     gene            complement(948..1709)
                     /gene="trpC"
                     /locus_tag="PL162_07805"
     CDS             complement(948..1709)
                     /gene="trpC"
                     /locus_tag="PL162_07805"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HQF91957.1"
                     /translation="MILERILERKRGEVAALRAPRRSLADAIASPGLAVIAEIKRASP
                     SRGVIRADGDPASWLRRYESGGAAAVSVVTDGPFFGGDGEMLRRLAPISSVPLLRKDF
                     IIDPLQIFESFFLGADAVLLIAAALEGEALPRMISQAKELGMEALVEVHDEAELERVL
                     ETEALLVGINNRDLTSFEVDLRTTERLVAHLDRREPGSRRLVVAESGVKNASDAHFLR
                     ECGADAILVGEALMRSEAPEALVAALRGSPASRTA"
     gene            complement(1706..2821)
                     /gene="trpD"
                     /locus_tag="PL162_07810"
     CDS             complement(1706..2821)
                     /gene="trpD"
                     /locus_tag="PL162_07810"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HQF91958.1"
                     /translation="MLKAFLEKVTAGKDLDGEEMEAAMGVVLEGGAPEAQVAGLLMGL
                     RCKGESVEEIVAAARALRHRALRVETERSPLLDLCGTGGDGSGTFNISTAASFVVAGA
                     GIPVAKHGNRSVSSRCGSADVLEELGVTFPGSPEEVRSSLATSGMAFLFAPHFHGTMR
                     QVAGVRRSLGVRTIFNVLGPLANPAGATHQLVGVFGAPLVRPMAEALSALGVQGAVVV
                     HGHGGVDELSLSGPNVVAVLRHGRITEERVSPEDVGLSRSPLEALRGGEARENAGVLR
                     GVLAGRPGPQRDAVVFNAAAALVAAGVERSWKEAAVRAQGSIDSGRAAEVLRRLSDFS
                     EEERQRRRLGDPCCGGGAVALASPGSRAFCPLVGVSA"
     gene            complement(2860..3420)
                     /locus_tag="PL162_07815"
     CDS             complement(2860..3420)
                     /locus_tag="PL162_07815"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HQF91959.1"
                     /translation="MILILDNYDSFTYNLADAFAPFDDVLVVRHDRIDPEGIDRLAPE
                     RIVLSPGPGRPEDSGVCPEAVRRFAGRVPLLGVCLGHQCIAQVFGGTVGRARAPRHGK
                     SSLVHHGGERLFRGMPSPFRAGRYHSLVVKGDAFPPELEVTAQTEEGEVMALAHRHLP
                     VFGVQFHPESVLTPEGRGIIRNFCGI"
     gene            complement(3417..4985)
                     /locus_tag="PL162_07820"
     CDS             complement(3417..4985)
                     /locus_tag="PL162_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HQF91960.1"
                     /translation="MARLGEAVMEKEAVAVMDRAGEGIPGEGFLLTEEDFLALAEEHR
                     IVPVGYRGPLEAPLREIYRRCRGGASFLLESDGTGPRSGRFAFVGTDPEVFFRSSERG
                     VELLRGDGTPQGPATEGKFFDLLRSHIEGGHGAGYPGLPPFFGGAVGYVAHEAVEDFD
                     DLFHDHPGRTLPRPPGPKALWFSPGTVLVVDQVLQTLTVICPVRVPRSSGEAERRDLH
                     RRARGRIEEVRRRIGRGSPSPTASLGMSPLVPRRSREEFLRMVARGRELIEEGELSQI
                     VLSQRFDAPCDEDPLELFAALRRANPSPYMFLLEFPGLAVVGASPEMLVRVRGRRVMT
                     RPLAGTRPRGGSVAEDRALERELRSDEKERAEHMMLVDLARNDLARVSVPGSVVVTEE
                     MGVERYARVMHLGSQVEGILRPECDALDALGAAFPAGTLSGAPKVRALERIAEIEGEP
                     RGVYGGAVGFLGFDGDLESAIAIRTVTLSQGVLSLQVGAGVVAESDPVREYEETMHKG
                     RALFEAFGGDEGSR"
     gene            complement(5319..6857)
                     /locus_tag="PL162_07825"
     CDS             complement(5319..6857)
                     /locus_tag="PL162_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91961.1"
                     /translation="MKKFFSMRIMGALWVMAMVLGSFPSWGSAPLGAGGGDTERYTYF
                     DNLPLFDPLTPLEPNEMRITFMGSMIPLPVRLAQAEMSIFVEVGWRGDASDPYYHGKP
                     VDQFIFDMGAGVSANYAAARVGYRLMDKVFINHLHADHMNDLLHTYCFGPSSDRKSPL
                     YVWGSKPSGVPIPSSRRFSAGGCDISGTMPDGAAYYDDGVKSFCSHLREALRWHTESF
                     SFQGTAYAGYVPPTRESWGLPHDPVPVGNDDPRDSYAIVPIELDWEKVGGVAYHNAST
                     GVEITHFPVIHCRKGSMGYKLTWRTPEGTDLSMIYTSDTKPELHSVEQAKNGGRGVDV
                     FIHEMVVPPAVWAYKSMGLNAAPEPGDPLYASFQETVQGLTRVQNSSHTSQGAFGYIL
                     SEIAPRPRLTVATHFPAADDTVASALESVKAHCPDVQMGRDIVWSFDLMVLRVFPHRI
                     EQCRADVSRFCFVPPVRIPDGILTPKYHDAEGKGDPFAQIDLSTEIPATNPDGTINYR
                     IDGY"
     gene            7648..8742
                     /gene="aroF"
                     /locus_tag="PL162_07830"
     CDS             7648..8742
                     /gene="aroF"
                     /locus_tag="PL162_07830"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HQF91962.1"
                     /translation="MLILDVDGRCPKGAIARMIQEVERHASYTRLVPAEEGYFILTDG
                     KREELSSLLSEPSVRRIVEAKNSFPLASTELWSGTRPVEVAPGLGIGKDSVAVIAGPC
                     AVESREQILASARAVQRAGAVALRGGAFKPRSNPYTFQGLAGDGVQLLCEARADTGLP
                     IVTEVMEPDQVGCLLPFVDVFQVGARNMQNFPLLKELGKTDRPVILKRGLAATVDEWL
                     QAAEYILAGGNFKVILCERGIRTFETRTRNTLDLSVVPLVKGLSHLPIVVDPSHGTGH
                     RPLVVPMALAALAAGADGLMIEVHPDPRRALSDGPQSLTPQEFSAFMPLASGVLAALG
                     RTLAGPQGTPEAPKMALDPVAAVPGGAPWN"
     gene            8733..9614
                     /locus_tag="PL162_07835"
     CDS             8733..9614
                     /locus_tag="PL162_07835"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HQF91963.1"
                     /translation="MELGAARIGIVGLGLMGASLAKKLTASGTVRRVTGWDRDGAVRH
                     LARSQGVAEVPEGLEELGSESDVLIFAVPWRFLADADRAVRRGLAKGGQTMWAVMDLC
                     SAKRAPGELLRRSWGGRYVGFHPMAGKERGGIIHATAELFSGAPCAIVSYPDTAPEVS
                     SFAARLAAALGMDPVEASPEDHDAAVAVVSHLPQLLASALALVAGEEARRNPLGARLA
                     GGGFRDTTRVASCPAEGGADLVSANGDHLRRGIGRLIQILEEFLSLPEEALEERLRAA
                     ASAREEILAIAAGRSAA"
     gene            9611..>10068
                     /locus_tag="PL162_07840"
     CDS             9611..>10068
                     /locus_tag="PL162_07840"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870360.1"
                     /note="catalyzes the formation of
                     5-O-(1-carboxyvinyl)-3-phosphoshikimate from
                     phosphoenolpyruvate and 3-phosphoshikimate in tryptophan
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HQF91964.1"
                     /translation="MKGIASTAPLRGTVVLPGDKSISHRAALLGAIATKGVEVTNYSE
                     GKDCVSTLTCLEKLGVRVLRGQESVSVERGRAFFDPDEPLDLGNSGTTARTLCGLLAG
                     MPGLYAVLTGDESLRRRPLRRVVDPLRVMGARIDGRHGGALLPLSVRGTRL"
BASE COUNT         1720 a         3621 c         3136 g         1591 t
ORIGIN      
        1 acctgcccca gggcgttatt gatcttgtgg gcccccgtgt ggttcagatc ctcccgcttc
       61 aggaaaaccc tcaccccccg ctgggccgac aggcggtggg cctcgtagat cggcgtggga
      121 cggccgctga agtcccgcag gagatccccc aggtcccgcc ggaagtctcc gtcgttcccg
      181 taccggtcaa aggccctttc cagctcctcc aagggaccca taagagtttc cggcacaaac
      241 tgccccccaa aggatccaaa gtatccccga cgccccgtcc ctcgatcacc catgaccccc
      301 actcccttct tcctcgctgg cgacctctga tcccgcccgg cgcgccgcct ccaccgcccg
      361 gcgcaccaat ccttcgtcct tttccccggg ggatcgttcc acctgactat taaaatccac
      421 cccccaggga cgcaccgctc gcaccgcctc ggcgacgttt tccggcccca accctcccgc
      481 cagaaggaag gggccccggg ggcggcggtg caagagcccc caatcgaagg gacgccccgt
      541 ccctccccgg gcgcccgggt ttcccgcgtc gaagagcgcc acgtcggcgc cctcgtagcg
      601 gagggcggcc tccacgtcgg cggcatcgcg gattccgaag gccttgatgg tcctcagagg
      661 gaaatcccgg aggagctccg ggggctcatc cccggaaaat tgaaccccgt cgaagaggcg
      721 cgacgccacg agctcccgga gctccccgtc ggtggggttc accacgaccc ctatcaccgt
      781 cgtgaagggg ggtaacccca ccgtgagctg gcgcacccgt tccaaagaca cgcgccgggg
      841 gctttcggcc aggataaatc ccaaggcatc gacgccctga cgcaccgccg ccgccacatc
      901 gccctcccgc gtcaggccac acatcttgat gcgaagggga aggggcatca tgccgtcctc
      961 gatgcggggc tcccccttag ggccgccacg agggcctccg gagcctccga tcgcatcagg
     1021 gcctccccca cgaggatggc atcggcgccg cactcccgca ggaaatgggc atcggaggca
     1081 ttttttaccc cgctttccgc caccaccagc ctgcgggatc cgggctcccg gcgatccaga
     1141 tgggccacga gccgttccgt cgtccgcaaa tccacctcaa aagaggtcag atcccggttg
     1201 tttatgccca ccaggagcgc ctcggtttcc aacacccttt cgagctcggc ctcgtcgtgc
     1261 acttccacca gggcctccat ccccagctcc ttcgcctggg agatcatccg gggaagggcc
     1321 tccccttcga gggccgccgc gatgaggagc accgcatcgg ccccgaggaa gaaactctcg
     1381 aagatctgga gggggtcgat gatgaaatcc ttacgcaaca gaggcaccga ggagatcggg
     1441 gccagcctcc ggagcatttc cccatcgccg ccgaaaaagg ggccgtcggt gaccaccgac
     1501 accgccgccg ccccgccgga ttcgtagcgg cgaagccaag aggcaggatc tccgtcggcc
     1561 cggatcacgc ctcggctcgg cgaagcgcgc ttgatctccg cgatgaccgc tagtccgggg
     1621 gaagcgatgg catcggccag ggatcggcgg ggggcccgca gagcggccac ctcccctcgt
     1681 ttgcgctcca ggatgcgctc caggatcatg ccgacacccc caccaggggg caaaacgccc
     1741 gggaacccgg ggatgccagg gccaccgccc caccgccgca gcagggatcc cccaggcgcc
     1801 gccgttggcg ctcctcctcc gagaagtccg acaacctcct caggacctcg gcggcgcgcc
     1861 ccgagtcgat gctcccctgg gcccggacgg cggcctcctt ccagctcctt tcgaccccgg
     1921 ccgccaccaa ggcggcggcg gcgttaaaca ccacggcgtc cctctggggc cccggtcggc
     1981 cggcgaggac cccccgcaaa accccggcgt tctcccgggc ctcgcccccc cggagcgcct
     2041 cgaggggaga cctggagagc ccgacgtcct cgggagacac cctctcctcg gtgattcttc
     2101 cgtggcgaag caccgccacg acgttgggtc ccgagaggga gagctcgtcc acccccccgt
     2161 ggccgtgcac caccacggcc ccctggaccc ccagggccga aagggcctcg gccatggggc
     2221 gcaccaaggg agcgccgaag acccccacca gctggtgcgt ggccccagcg gggttcgcca
     2281 ggggacccag gacgttgaag atggtccgca ccccgaggga ccggcggacc cccgccacct
     2341 gcctcatggt gccgtggaag tgtggggcaa agagaaacgc catgccggag gtcgccaggc
     2401 tgcttcgcac ctcctccggc gacccgggaa aggtcacccc gagctcttct agcacgtcgg
     2461 cgctaccaca tcggctggaa acggatcgat tgccgtgttt ggccaccggg atccccgccc
     2521 ccgccaccac gaaggacgcg gcggtggaga tgttgaacgt acccgatcca tccccccccg
     2581 tgccgcagag atccagcagg ggagaacgct ccgtctccac ccggagggcc cgatggcgca
     2641 aggcccgagc cgccgcgacg atctcttcca ccgactcccc cttacacctc aatcccatga
     2701 gcaaccccgc cacctgggcc tccggcgccc ccccttcgag gacgaccccc atggcggcct
     2761 ccatttcctc cccgtccagg tcctttcccg ccgtcacttt ctccaaaaag gctttcagca
     2821 tgctcgtctc atcctttctc ttctcgacct atcggatact taaataccgc agaagttcct
     2881 gatgatgccc cggccctcgg gggtcaagac cgactcgggg tggaactgga ccccgaagac
     2941 cggaaggtgg cggtgggcca gggccatgac ctctccctct tccgtttgcg ccgtgacctc
     3001 cagctccgga ggaaaggcat cgcctttcac caccaaagag tggtaccttc cggcccggaa
     3061 ggggctcggc atcccccgaa agaggcgctc tccgccgtgg tgtaccaaag aactcttgcc
     3121 gtgacgcgga gcccgggccc gtcccaccgt gccgccgaag acctgggcga tgcactggtg
     3181 ccccagacac acccccagca ggggcacccg gcccgcgaag cgccgtacgg cctcggggca
     3241 gacccccgaa tcctccgggc gccccggccc cggcgacagg acgatccgct ccggggcgag
     3301 gcgatcgatc ccctccggat cgatgcggtc atggcgcacc accaggacgt cgtcgaaggg
     3361 ggcaaaggcg tccgccaggt tgtacgtgaa ggagtcgtag ttatccagga tgagaatcat
     3421 cgcgacccct catccccgcc gaaggcctcg aagagggccc tccccttgtg catcgtctcc
     3481 tcgtattccc ggaccggatc cgactccgcc acgacccccg cccccacctg gagggagagc
     3541 actccctggc tcagggtgac cgtgcggatg gcgatggcgc tctccaggtc accatcgaag
     3601 ccgaggaatc ccaccgcccc gccgtagacc ccccggggct ccccctcgat ctcggcgatg
     3661 cgctccaggg ctcggacctt cggcgccccg gagagggttc ccgcaggaaa ggcggccccc
     3721 agggcgtcca gggcgtcgca ctccggacgg agaatcccct ccacctggga tcccaggtgc
     3781 atgacgcggg cgtagcgctc cacccccatc tcctcggtca ccacaacgct ccccggcacc
     3841 gagacccggg ccaggtcgtt ccgcgccaga tccacgagca tcatgtgttc ggctcgctcc
     3901 ttctcgtcgc tccggagctc ccgctccagg gcccgatcct ccgccacgga gcccccccgg
     3961 gggcgggtcc ccgccaaggg acgagtcatg acccggcgcc cccggacgcg gaccagcatc
     4021 tccggcgatg cccccaccac ggcaaggccg ggaaactcca ggagaaacat gtacggcgag
     4081 gggttggccc gacggagggc cgcaaagagc tccaggggat cctcgtcgca gggggcgtcg
     4141 aagcgctggg acaggacgat ctgagaaagt tccccttcct cgatgagctc cctacctcgg
     4201 gccaccatcc ggaggaactc ctcccgggac cgccgaggga ccagggggct catcccgagg
     4261 gaggcggtgg ggctaggaga tcctctgccg atgcgcctcc ggacctcttc gatccgcccc
     4321 cgggcccgcc ggtggagatc gcggcgctcg gcctcccccg agctccgggg aaccctcacg
     4381 ggacagatca cggtgagggt ctggaggacc tggtccacca ccaacaccgt gccgggggaa
     4441 aaccagaggg ccttgggccc cggcggccgg ggaagggttc tcccgggatg gtcgtgaaag
     4501 aggtcgtcga agtcctccac cgcctcgtgg gccacgtatc ccaccgctcc gccgaaaaag
     4561 gggggaagcc ccgggtatcc cgccccatgc cccccttcga tgtggctccg gagcaggtca
     4621 aaaaacttcc cctccgtcgc gggaccctgg ggggtaccat cgccccggag gagctccact
     4681 ccccgctccg aactccggaa aaacacctcc ggatcggtgc cgacgaaggc aaatcggccc
     4741 gatcgggggc ccgtgccatc gctttccagg agaaaggacg cgccgccgcg acacctccgg
     4801 tagatctccc gcaaaggggc ctccaggggc cctcggtacc ccaccggcac gatgcggtgc
     4861 tcctccgcca gggccagaaa gtcctcttcg gtcaggagaa acccctctcc cgggatccct
     4921 tcgcccgccc tatccatcac cgccaccgct tctttctcca tcaccgcttc gccaagcctt
     4981 gccatctcgt ctcgatctcc cttcctgcct cttctcgtcc gctccgccga gggccttcgc
     5041 ggcttcggcg caaaaaaatc gagggagtcg tcctctccga cgaccccctc gatgctcccc
     5101 cggcggggag taagaaaaaa gggctacgga gagggtttaa tccctccgca gcccggccat
     5161 gcatcaggcg tgcggcggga gcctagggct cccaccacca aaggttcgct accgtacgga
     5221 ccgtctgaac cgcagtacgc ctcatgccat ctcccccaat cttgcggggg atcctagcac
     5281 actcctcccc gaagcgcaag ggggtggaaa aattaatatc aatagccgtc aatccggtag
     5341 tttatcgtgc cgtccgggtt agtcgccgga atctccgtcg acaggtcgat ctgggcaaag
     5401 gggtcaccct ttccttcggc gtcgtggtac ttgggcgtca ggatcccgtc ggggatccgg
     5461 acggggggaa cgaagcaaaa tctcgacacg tcggcgcggc actgttcgat gcggtgcggg
     5521 aacacgcgca acaccatcag gtcgaaggac cacacgatgt cgcgccccat ctggacgtcc
     5581 gggcagtggg ccttgacgct ctccagggcg ctggccacgg tgtcatcggc ggcgggaaag
     5641 tgggtggcca ccgtgagccg ggggcgaggg gcaatttcgc tgaggatgta cccgaaggcc
     5701 ccctgggagg tgtgcgagct gttttggacc ctcgtcagtc cctggacggt ctcctggaac
     5761 gaagcgtaaa ggggatcgcc gggctccggg gccgcgttga gtcccatgct cttataggcc
     5821 cagaccgcag ggggcaccac catctcgtgg atgaacacat ccaccccccg gccgccgttt
     5881 ttggcctgtt cgacgctgtg gagctcgggc ttcgtgtcgc tcgtgtagat catggacagg
     5941 tcggtgccct ccggagtccg ccaggtgagc ttgtacccca tcgacccctt tcggcagtgg
     6001 atcacgggaa agtgggtgat ttccactccc gtggaggcgt tgtggtaggc cactcctccg
     6061 accttctccc agtccagctc gatcggcacg atggcgtagg aatctcgggg gtcgtcgttt
     6121 cccaccggca ccgggtcatg gggaaggccc cagctctccc gggtgggggg cacgtagccc
     6181 gcgtaggcgg tgccctgaaa gctgaagctc tccgtgtgcc accgcaaggc ctctcgcagg
     6241 tgcgaacaaa agcttttgac tccgtcgtca tagtaggccg cgccatcggg catcgtgccc
     6301 gaaatgtcac atcccccggc ggagaaccga cgggaagaag ggatcggcac gccggagggc
     6361 ttcgagcccc agacgtaaag gggggacttc cggtccgacg aggggccgaa gcagtaggtg
     6421 tggagcaggt cgttcatgtg gtcggcgtgg agatggttga tgaacacctt gtccatcagg
     6481 cggtatccca cccgggccgc cgcgtagttg gccgacaccc ccgcccccat gtcgaagata
     6541 aactggtcca ccggcttgcc gtgataatag ggatcgctgg catcgccgcg ccatcccacc
     6601 tcgacgaaga tgctcatttc ggcctgggcc aggcgcaccg gcagggggat catggagccc
     6661 atgaaggtga tacgcatctc gttgggctcc aggggagtca ggggatcgaa aagcggcaag
     6721 ttgtcgaaat aggtgtaacg ctcggtatcc cctccccccg cccccagggg agccgacccc
     6781 catgagggaa aagatcccag gaccatcgcc atcacccata acgctcccat tatccgcatg
     6841 ctaaaaaatt ttttcataat cccattcccc cttgttcctt ctcctcatcg cggaaaaaac
     6901 cttggctcgg aggcccccac gccaccgcag gcgtcccgaa ccatccctca tcaaaccctc
     6961 ccccgacggc tctatcacct tctctacaag atctccgcat tgcccccaga aatagcactc
     7021 cggaaaaaat ccgtcaaccg gcaaagatct ccggggaccc agggtctttt ggggcgccga
     7081 gggcgatccc aagccccggg gctttggccc cggggggcga tggggccttc gggaccgggg
     7141 aggtcgcttc gaaaaaaaat tacccccttg acgaaaaatc caaaggcgtg taaggtgtcg
     7201 cccaacgttt cccgatgaac ttccccagga ggtgacacag tggttcggac caaggccccc
     7261 cttgaccccc tattccggtc tctttccgcc gacgccccgg cgccgctgcg tcccctgggg
     7321 ctttcacccc ggcgaggcct cggcggcctg cctccttccg ttgcgggaaa ccttccctcg
     7381 cagcgcggga ctgattcccc ccttcatgcc ctctattacg tggggttctc ctttaccgac
     7441 ggccagtggc gttatcgcca gtggcccttc gtctatcccg aaaccgatta tcggcaaagg
     7501 agatcttctc tccccccaaa cccccgggac ctctgatcct cccccggaag ggacacgcca
     7561 cattaaaagg gccgagttcg gtttcatccg aacccggccc tttttctttt tcgtgaagat
     7621 gaaaaacttt caggaggtgc gtaaaagatg ttgatcctcg acgtggacgg ccgttgtccc
     7681 aagggagcca tcgcgcggat gattcaggaa gtggagcgcc atgcctcgta cacgaggctc
     7741 gtgcccgccg aggagggata ctttatcctg acggacggca aaagggagga gctgagttcc
     7801 ctcctctccg agccttcggt gcgccgcatc gtcgaggcaa aaaactcctt tcccctcgcc
     7861 agcacggagc tctggtcggg gacgcgcccc gtggaagtgg cccccggcct cggcatcgga
     7921 aaggactccg tggcggtcat cgccgggccc tgtgcggtgg aaagccgaga gcagatcctc
     7981 gcttccgccc gggcggtgca gagggcaggg gccgtggcgc tccggggcgg ggccttcaag
     8041 cctaggtcca atccctacac cttccagggc ctcgccggag acggggtgca actcctgtgc
     8101 gaggcccgag ccgacacggg acttcccatc gtcaccgagg tgatggaacc cgatcaggtg
     8161 gggtgcctcc tgcccttcgt cgacgtcttc caggtcgggg cccggaacat gcagaacttc
     8221 cctctgctca aggagctggg caagaccgat cgccccgtca tcctcaagcg gggcctcgcc
     8281 gccacggtgg acgagtggct ccaggccgcc gagtacatat tggcgggggg caacttcaag
     8341 gtcatcctct gcgagcgggg catccgcacc ttcgagaccc gcacgcgcaa taccctcgac
     8401 ctgagcgtcg ttcccctggt gaaggggctg agccacttgc ccatcgtcgt cgacccgagc
     8461 cacggcacgg ggcaccgccc cctggtggta cccatggccc tcgccgccct cgccgccgga
     8521 gccgacggcc tcatgatcga ggtgcacccc gatccccgcc gggccctctc cgatggtccc
     8581 cagtccctca cgccccagga gttctccgcc ttcatgcccc tggcatcggg ggtcctcgcc
     8641 gccctcggac ggaccctcgc cgggccccag ggaactccgg aggcgccgaa aatggcactg
     8701 gaccccgtcg ccgcagtccc gggaggggca ccatggaact aggggccgca cgcatcggca
     8761 tcgtgggatt ggggctcatg ggggcttcct tggcaaaaaa gctgacggca tcggggaccg
     8821 tgcggcgcgt gacggggtgg gatcgggacg gtgcggtgcg gcacctcgcc cggtcccagg
     8881 gggtggccga ggtgccggag gggctggaag agttgggatc cgaaagcgac gtgctgatct
     8941 ttgccgttcc ttggcgcttc ctggccgatg cggaccgggc ggtgcgaagg ggccttgcga
     9001 aaggggggca aacaatgtgg gcggtgatgg acctttgcag cgcgaaacgg gcgccggggg
     9061 agctcctcag gcgctcctgg ggggggcgat acgtgggctt tcaccccatg gcgggcaagg
     9121 agcggggagg gatcatccac gccaccgccg agctcttttc cggcgccccc tgcgccatcg
     9181 tctcctaccc cgatacggcg ccagaggtga gctcctttgc cgcccgcctc gcggcggccc
     9241 tggggatgga ccccgtggag gcgagccccg aggatcacga tgccgccgtg gccgtcgtga
     9301 gccaccttcc tcagcttctg gcctcggccc tggccctcgt ggccggggag gaggcccgtc
     9361 gaaaccccct gggggccagg ctcgccggag ggggtttccg ggacaccacc cgggtggcct
     9421 cctgccccgc tgaagggggc gcggacctgg tgagcgccaa tggggatcat ctccgccggg
     9481 gaatagggcg cctgatccaa attctggaag agtttctctc cctccccgaa gaggccctgg
     9541 aggagcgcct tcgcgccgcc gccagcgccc gggaagaaat tttagccatc gccgcaggaa
     9601 ggagcgccgc atgaaaggga tcgcatcgac cgcccccctg aggggcaccg tggttttgcc
     9661 cggggataaa tccatctccc accgggcggc cctgctcggg gccatcgcca cgaagggcgt
     9721 ggaagtcacc aactactcgg aggggaaaga ctgcgtctcc accctcacct gcctggaaaa
     9781 gctgggggtt cgggtcctcc ggggacagga aagcgtctcc gtggagcggg gaagggcctt
     9841 tttcgacccc gacgagcccc tcgacctggg aaattccggc accacggccc gcacgctctg
     9901 cggcctcctg gcggggatgc ccgggctcta cgcggtcctc accggggacg agagccttcg
     9961 gcgacgcccc ctgcggcgcg tggtggaccc cctgcgggtc atgggggcgc gcatcgacgg
    10021 acgccacggg ggggccctcc tcccgctgag cgtccggggc acccgcct
//
LOCUS       DAOOER010000064         9954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_7258_length_9954_cov_7.768866, whole genome shotgun sequence.
ACCESSION   DAOOER010000064 DAOOER010000000
VERSION     DAOOER010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 9954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9954
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7258_length_9954_cov_7.768866"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PL162_07845"
     CDS             complement(<1..637)
                     /locus_tag="PL162_07845"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HQF91965.1"
                     /translation="MRVVDVSALTDLVRNLALEANFRLPPDVIRALEEAHSREISPGG
                     RRVLADLRENGRVAQEEGLPLCQDCGLAVVFLDVGEEVAFSGKGVEEAVHEGIRQAYR
                     QGYLRRSVVSDPLFDRRNTGDNTPAILHLRWVPGDRIRVTVAPKGMGSENMSALKMLR
                     PVDGEEGVRAFVVETVAAAGPNPCPPVVVGVGIGGNFETAPRAAKRALLRPC"
     gene            complement(690..2405)
                     /locus_tag="PL162_07850"
     CDS             complement(690..2405)
                     /locus_tag="PL162_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQF91966.1"
                     /translation="MGKRLMVKLVAPLVLLVALLGGGFLVTMGILESQKDDGLLVNLA
                     GRQRMLSQRMSKEFFAFLVTGDEALKGKIEQGIKVFDATMKALSRGGLAPLDMAFSRT
                     AELPQAPSELRERLDAAEKMWGPFQEHLRAVNRSDLLQDKALIDTVKQENEVLVGALD
                     ALTSEFQKVAEDRVSQIILVQEGLTVLGLLILGLCLVFYTRSILHPLRQLGAMAQELS
                     SGDADLTRRLPVTTEDEIGRLARDFNAFLETLGDLVSQVKEESEALARKVESVDSSAR
                     SMEEMASLLAESGTKLKGVVAQNSEAVETINAGMEEVASGTQTAAYSSSSAAEGAEGV
                     RALAQEAQEKVKVLGEAAQSLADQTRSTGGKVSTVQGFAQEIGKLVDVIARIADQTNL
                     LALNAAIEAARAGEAGRGFAVVAEEVRKLAEESNGAAKNIVGLVSHIRGSVGEAVVAM
                     GETGEAVSSSVSQVGTVRSSLETLLHKVEQIVDAVQDIAAVSEEQSASAEEVAASTDK
                     IAAFSGRTSDEADHLAVMAADLSRLVETVSEAVRLLSERSHNLERLTAGFHTAPRTGV
                     RALSR"
     gene            2551..3045
                     /locus_tag="PL162_07855"
     CDS             2551..3045
                     /locus_tag="PL162_07855"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative metallopeptidase"
                     /protein_id="HQF91967.1"
                     /translation="MEEIFGFEVCNERFRPMAEALRRKYEELRHVDPESVLFLMNRKS
                     LGKQKKRVVLARTSKVPPKWQEVLYQLGGGSYFFMVEFYEKSLEPLDQAQITALIYHE
                     LRKITPEGGVVPPDVHDWYQMIQGLGRHWFYPDATCPDLLAEGVDWKKLMGSFYEAPH
                     PSES"
     gene            3058..4086
                     /gene="nadR"
                     /locus_tag="PL162_07860"
     CDS             3058..4086
                     /gene="nadR"
                     /locus_tag="PL162_07860"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF005988.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HQF91968.1"
                     /translation="MTLGIFPGKFLPLHRGHLAGILQAHTLVDRLVVVLSWSPEDDAI
                     CLRDGLRRPISEVQRKQWLRQELQGFENIDVRSVDESTVPPFPGGWEEWALRVQEAVG
                     EPIDLIFGGEPEYAVSFRHFFPGAQYVMVDPHRGQWNISGTSIRKNPLRYWDFICGSA
                     RPFFVQKILVSGTESTGKTTLVKKLAKIFHTSWSEEVGRYYAQQHLGGDETIFTPMDF
                     ARMAHLQYEEDYKAMRTANRVVFFDTDAVVTNYYSELYMGARNPRVGAFVDPARYDVT
                     FLLTPSVPWVPDGQRIHGAEPERWRLHAMLRRMYEEYGFSPVVEVSGNYNERFSTVYN
                     AVFDMYTP"
     gene            complement(4110..5420)
                     /locus_tag="PL162_07865"
     CDS             complement(4110..5420)
                     /locus_tag="PL162_07865"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQF91969.1"
                     /translation="MSAEKGMSSVRLYVRGMHCPSCEMMAESRLGSQSGILGAKASFR
                     DGTVVFETNCPEKWTPEALGDLFRDQGYRFSREEPSKGSRVAALLGILGVVGVIVLSA
                     RWDLGALIDLSPTSSLGMVALFGVLAGCSSCAALVGGMVWALAPRWRGKGAHGAFAAG
                     RLGAYLVGGALLGGLGAEVRPSPMLSSVLVFGVALVMAGLGLSMMGLPGWHALPLPGG
                     RMGRRFLEAQGLSDKGPWLAGVVSLFLPCGFSLTVQGLALVSGSPWRGAALMGVFALG
                     TLPSLAIIGLSGASWGGAPRAWGRRVAGALAVAAAVWSANTQLNVLGVPSLSDLGRPV
                     AASPEISPSPVDSGPQELTMEVGGRGYSPRHFTVKAGVPVRWHIVDRGFSGCTNAIQA
                     PGLFDGVLPLVRGGTATVEFTPESPGTYKFACWMGMATGEIQVE"
     gene            complement(5580..8108)
                     /locus_tag="PL162_07870"
     CDS             complement(5580..8108)
                     /locus_tag="PL162_07870"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HQF91970.1"
                     /translation="MDQGKGSSHRGERKIVLDVEGMHCASCVGNVERALKGVAGVRDA
                     VVNLATRRASVEASEEVSIDELVKAVERLGFAASAREEAPPSLSRKVFDVEGMHCASC
                     AMNLERRLRQVAAVSSVAVNFPQKRAVVDLTGVVEPGALERAAQELGFRMEPHRERRA
                     EAEDYAARELREARRRFIWGWILAVPSMVIMVLHEFGINVPSDLTQHLLLEQFLSFLV
                     IFVAGGDTILRAVRQGVRGHTSMDTLIALGTGASFASGLLKLAGWPVTSFAMVGGMIM
                     AFHLTGRYLETGARSRATREIAKLVEMGARTARVERPEGEVEIPVEDLVPGDLLVVRP
                     GEKIPQDGEIVEGVASVDEAMISGEPLPVVKSPGDEVTGATVNVDGFLKIRVTRVGGA
                     SFLSQIIALVSEAQGTKVPIQAFADRVTSVFVPVVVSLAVGSFVLLYGAPGIFEGLWG
                     AVAPFFPWVDPGAPVLSRALSAAIATLVIACPCALGLATPTALMVGTGLSARRGILFR
                     NGEAVQTMKAVDVVLLDKTGTLTTGKPSVERLVLEGETAAEELLRAALALEDRSEHPL
                     SRAVVAFARQQGVTSAEVEVEVEDFAAVPGRGVQGRVGRKMCRIGSLAWLAEKGMTLP
                     EELRRAAEEGQVSGATPLVFFDDAVRALFLVTDAVKSESAGAVRRLREMGKHTVLLTG
                     DTRGAAERVGTLLGVDEIVAEVRPQDKMAVVERFQADGRTVAMVGDGINDAPALKQAH
                     VGIALGTGTDIAREAGDVVLVRGNLEALGEAFFISDAMFRKIRQNLFWAFFYNVVALP
                     LAFLGLLHPLMAEAAMALSSLNVVGNSLRLRRLMPQGEDPAARR"
     gene            8415..9185
                     /locus_tag="PL162_07875"
     CDS             8415..9185
                     /locus_tag="PL162_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQF91971.1"
                     /translation="MRENLLFVDDLHVTREGNPILKGVTLRVNPGEIVGVLGRNGAGK
                     SSLAYALMGTHRYTPSSGKIFFAGEDITGEPISQRAKRGLTLAWQHPARYEGITVRQY
                     LRLSAQGVKEADMEDALSRVQLETPYLERMVDKALSGGERKRIELASIFLMQPRLAML
                     DEPDSGVDLIALGDIMHLFRDLAGGTNGVLLITHREDVAAACDRSYLMCNGRIILEGS
                     AEAVKRYFMSQCSPCTETPLLPVAPELPAALPVPKEVC"
     gene            9188..>9954
                     /locus_tag="PL162_07880"
     CDS             9188..>9954
                     /locus_tag="PL162_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HQF91972.1"
                     /translation="MFSVQDEYKTLVDIAERTAGEGSGSLLGQDDAVYVVVHANKVLA
                     HHELPGVTIEADELPDGIAARITVADGTVIAKPVHLCFGHLGHEGRQTIQSRVHIGKG
                     ARATFLAHCIFPNAIEFLHAMEGDLVVDEGGDFTYNEVHVHGPEGKITVRPVTRVTLE
                     RGAIYRGDFTLVEGRVGDLDIHIDVEARGERSRVEITSKVYGKDDDQCQVRDVVRLSG
                     EGSTALIKARVVLRDRSEGRFLGLIEGGAAGARGHVD"
BASE COUNT         1887 a         3538 c         2903 g         1626 t
ORIGIN      
        1 cgcaggggcg caacagagcc ctcttggccg cccggggggc ggtttcgaag tttcccccga
       61 tgcccacccc caccaccacg ggggggcagg ggttgggccc tgcggcggcc acggtctcca
      121 ccacgaaggc ccgcacccct tcttccccgt cgacggggcg cagcatcttc agggcgctca
      181 tattctcgct tcccatgccc ttgggggcca ccgtgacccg gatccgatct ccgggcaccc
      241 accggagatg gagaatggcc ggggtgttat cccccgtgtt tcggcggtca aagagaggat
      301 cggacaccac ggatcttcgg agatagcctt ggcgataagc ctggcgaatg ccctcgtgca
      361 ccgcttcttc caccccctta ccggagaagg ccacctcttc tcccacgtcc aaaaagacca
      421 ccgccagacc gcagtcctga cacaggggca agccctcttc ctgggccacg cggccatttt
      481 cccggaggtc ggccagcacc cgcctccccc cgggggagat ctcccgagaa tgggcctcct
      541 ccagggcccg gatcacgtcc ggaggcaggc gaaagttggc ctccagggcc agatttctca
      601 ccaggtcggt cagggcactc acgtccacga cgcgcatgaa aatcctcccc taaaaaagat
      661 cttacggcgt tccgcccttc cccccaggtc taccgggaca aggcccggac ccctgttctg
      721 ggggccgtgt ggaagcccgc cgtgagcctc tccaggttgt gcgatctctc cgatagaagc
      781 ctcacggcct cggacaccgt ttccaccagg cgggacaggt cggcggccat gaccgccaga
      841 tggtccgcct catccgaggt ccgcccggag aaggcggcga tcttgtccgt ggaggccgcc
      901 acctcttcgg cgctggcgga ttgttcttcg ctcaccgccg cgatgtcttg cacggcgtcc
      961 acgatctgct ccaccttatg caggagcgtc tccaggctcg accgcacggt gcccacttgg
     1021 gacaccgacg aggacaccgc ctctcccgtt tcccccatgg ccaccaccgc ttctcccacg
     1081 gagccccgga tgtggctcac caggccgacg atgtttttgg cggcgccgtt ggattcctcc
     1141 gccagcttgc gcacctcttc cgccaccacg gcgaagcccc gtcccgcctc ccccgcccgg
     1201 gcggcctcga tggccgcatt cagggccagc aaattggtct ggtcggcgat ccgggcaatg
     1261 acatccacca acttgccgat ctcctgggca aagccctgca ccgtggagac cttgcctccg
     1321 gtggaacggg tctgatccgc caggctctgg gccgcttctc ccaatacttt gaccttttcc
     1381 tgggcctcct gggccagggc ccgcacccct tccgccccct ccgccgcgct ggagctggaa
     1441 taggctgcgg tctgggtgcc cgaggccacc tcttccatgc ccgcgttgat ggtctccacg
     1501 gcctcgctgt tttgagccac cacgcctttc agcttggtgc cgctttccgc cagaagggag
     1561 gccatctcct ccatggatcg ggcggaggag tccacgctct ccacctttcg agccagggcc
     1621 tcggattctt cttttacctg ggacaccagg tcccccaggg tctctaaaaa ggcattgaag
     1681 tccctcgcca gccgccctat ttcgtcctcg gtggtcacgg gcagacgccg ggtgaggtcc
     1741 gcgtcgccac tggatagctc ctgggccatg gctcccaact ggcgcaaggg gtgcaaaatg
     1801 gatcgggtgt aaaagaccag acagagcccc aggataagga gccccaacac cgtcagtccc
     1861 tcctgcacga ggatgatctg agacacccga tcctcggcca ctttctggaa ttccgacgtg
     1921 agggcgtcca gggcccccac caggacttcg ttttcctgct tcaccgtatc tatcaaagcc
     1981 ttatcttgga gcaaatcact gcgattaacg gcccgaagat gctcctgaaa ggggccccac
     2041 atcttctccg ccgcatccag gcgctcccgg agctccgacg gcgcctgagg gagctccgcc
     2101 gtgcgggaaa aggccatatc caggggggcc aagccccctc gggatagggc cttcatggtg
     2161 gcatcgaaga ccttgatccc ttgctctatc ttacccttaa gggcctcgtc ccccgtcacc
     2221 aagaaggcaa aaaactcctt ggacatgcgc tgggagagca tgcgctgcct tcccgccagg
     2281 ttcaccagca gcccgtcgtc cttttgggac tccagaatgc ccatggtgac gaggaatcct
     2341 ccccccagca gggccaccaa aagcaccagc ggagccacga gcttcaccat cagccgtttt
     2401 cccacgagtt atcccccttt tctactcttc gaggggcccc ttcgaggggc ctcttccaaa
     2461 gctgctcccc attttacccg aaattctgcc gccgaagggc ctcgggcgta ttataatgcg
     2521 aggcgagaat gacggggagg tggatgcaaa gtggaggaga tcttcggctt cgaagtgtgc
     2581 aacgaacgat tccgccccat ggccgaggcc ctgaggcgca agtacgagga actgcggcac
     2641 gtggatcccg aaagcgtact gtttttgatg aaccgcaaga gcctggggaa acagaaaaag
     2701 agggtggtgc tggcccggac ctccaaggtt ccccccaagt ggcaggaggt gctctaccag
     2761 cttggcggag gctcgtactt cttcatggtg gagttctacg aaaagagcct ggagcccctg
     2821 gatcaggccc agataaccgc cctgatctat cacgaactcc gcaagattac ccccgaaggg
     2881 ggcgtggtgc cccccgacgt ccatgactgg taccagatga tccagggact gggacgccac
     2941 tggttctatc ccgacgccac ctgcccggac ctcctggcgg agggcgtgga ctggaaaaag
     3001 ctcatgggat ccttctacga ggctccccac ccctcagaaa gctaggagga atcgaccatg
     3061 accctgggca tctttccggg aaagtttctc cccctccacc gggggcatct ggcggggatc
     3121 ctccaggccc ataccctggt ggaccgcctg gtggtggtcc tatcctggag ccccgaagac
     3181 gacgccatct gccttagaga cggcctgcgt cgccccattt ccgaggtgca gcgaaaacaa
     3241 tggctccgcc aggagcttca gggcttcgag aacatcgacg tgcgctccgt ggacgaatcc
     3301 accgtgcccc cctttcccgg agggtgggag gagtgggccc ttcgggtcca agaggccgtg
     3361 ggggagccca tagatctgat cttcggcgga gaaccggagt acgccgtgag ctttcgccac
     3421 ttcttccccg gggcccagta cgtcatggtg gacccccacc gggggcagtg gaatatctcc
     3481 ggcaccagca tccgcaagaa ccccttgcga tattgggatt ttatctgcgg ctccgcccgt
     3541 cctttcttcg tgcaaaagat cctggtgagc ggcacggaat ccacgggaaa aaccactttg
     3601 gtgaaaaagc tcgccaagat ctttcacacc tcctggtcag aagaggtggg gcgatactac
     3661 gcccagcagc acctcggcgg cgacgagacc atctttaccc ccatggactt cgcccgcatg
     3721 gcacacctcc agtacgagga agactataag gccatgcgca ccgccaaccg cgtggtcttc
     3781 ttcgacaccg acgccgtggt gacaaactac tattccgaac tatacatggg ggcccgcaac
     3841 ccccgggtgg gggccttcgt cgatcccgcc cgatacgacg tgacctttct gctcaccccc
     3901 agcgtcccct gggtacccga cggccagcgc atccacgggg cggagcccga acgctggcgc
     3961 ctccacgcca tgctgcgccg gatgtacgaa gaatacggct tttcccccgt ggtggaggtc
     4021 tccgggaact acaacgaacg tttcagcacg gtctacaacg cggtcttcga catgtacact
     4081 ccgtgaggcc accccgcccc tcgaaagggt cactccacct gaatctcccc cgtggccatg
     4141 cccatccagc aggcaaactt gtatgtcccg ggggattcgg gggtaaactc caccgtggcg
     4201 gtgcctcccc gcaccagggg gagcacgccg tcaaagagcc ccggagcctg gatggcgttg
     4261 gtacacccgc tgaagcccct gtccacgatg tgccatcgca cgggaactcc cgctttcacc
     4321 gtgaaatggc gaggggagta accccgtccc cccacctcca tggtgagctc ctgggggccg
     4381 gaatccaccg gggacgggga gatttcgggc gacgccgcga cggggcgccc caggtcggat
     4441 aggctgggga cccccaggac gttcagctgg gtattggcac tccataccgc cgccgccacc
     4501 gccaaagccc ccgccaccct ccgtccccac gccctcgggg cccctcccca cgaggctccg
     4561 gaaaggccga tgatggccag agaaggcagg gtccccaagg caaagacccc catgagcgcc
     4621 gccccccgcc aggggctccc cgagaccagg gccaacccct gcaccgtcag ggaaaaaccg
     4681 cagggcagaa aaagcgatac cacccccgcc agccagggac ccttatccga gagtccctgg
     4741 gcttccagga aacgcctccc catcctaccc cccggcaggg gaagggcatg ccaccccgga
     4801 aggcccatca tggaaagccc cagtcccgcc atgacgaggg ccacgccaaa caccaatacc
     4861 gacgaaagca tcggcgaagg gcgcacctcc gcccccaatc cccccaggag cgcccctccc
     4921 acgagatagg cccccaggcg tccggcggcg aaggctccgt gggccccttt gcctcgccac
     4981 cggggggcca gggcccacac cattcccccc accagggccg cgcaggagga gcatcccgcc
     5041 aggaccccaa agagggccac cattcccaga gacgaggtcg gagacaggtc tatcagggcc
     5101 cccagatccc atcgggcgga gaggacgatc acccctacca ccccaaggat tcccaaaaga
     5161 gccgcgaccc gagacccctt ggagggctcc tcccgggaga agcggtaccc ctgatcccga
     5221 aaaagatctc ccagggcctc gggggtccat ttttccgggc agttcgtctc aaaaaccacc
     5281 gtgccgtcgc ggaaagaggc cttcgcccct aaaatgcccg actgggagcc taaccgggac
     5341 tccgccatca tctcgcaaga ggggcagtgc ataccccgga cgtaaagacg caccgacgac
     5401 atccccttct cggcgctcat cgaggccccc tggggcttca aagtcttttt cggcgaagag
     5461 cccatccaaa gtcatccctt tctggtcttt caaaacttcc catgcccttc tcccctcggc
     5521 agggccgatt agaccccggc cgggggcacc gatggcggaa aataaaggcc cccgggccgc
     5581 taccgacgag cggcagggtc ctctccttgg ggcatcaggc gccgcagacg cagggagttg
     5641 cccaccacgt tcaatgagct cagcgccatg gcggcttccg ccatgagggg gtgcaaaagc
     5701 cccaggaagg ccaggggcag agccaccacg ttgtaaaaaa acgcccagaa gaggttttgg
     5761 cgaatcttcc ggaacatggc gtcggagatg aaaaaggcct ctcccagggc ttccagattt
     5821 ccccggacca acaccacgtc ccctgcctcc cgggcaatat ccgtccctgt ccccagggcg
     5881 atgcccacgt gggcctgctt cagcgccgga gcgtcgttga tgccgtctcc caccatggcc
     5941 accgtccgac catcggcctg gaaacgctcc accacggcca tcttatcctg gggacgcacc
     6001 tccgccacga tctcgtccac ccccaggagg gtccccaccc gctccgccgc cccccgggtg
     6061 tctcccgtga ggagcaccgt atgcttgccc atctcccgga ggcggcgcac cgctccggcg
     6121 ctctcggatt tcaccgcatc cgtcaccaga aagagggccc gcaccgcgtc gtcgaagaac
     6181 accaggggcg tggcccccga cacctgtccc tcctccgccg cccgccgtag ctcctcgggc
     6241 agggtcatcc ccttttcggc gagccaggcc aagctcccga tgcggcacat cttacgcccc
     6301 actcgcccct gaaccccccg ccccggcacg gcggcaaagt cctccacctc cacctccacc
     6361 tccgccgacg tcaccccctg ctgtctcgca aaggccacca ccgcccggct taaggggtgc
     6421 tccgatcgat cttccagggc cagggccgcc cggagaagct cttcggccgc cgtctccccc
     6481 tctagcacca ggcgttccac ggagggcttc cccgtggtga gggtgccggt tttatcgagc
     6541 agtaccacgt ccaccgcctt catggtctgc accgcctcgc cgtttctgaa caggatcccc
     6601 cgccgggcgg agagccccgt gcccaccatg agggccgtgg gggtggcgag ccccagggcg
     6661 caggggcaag cgatgaccaa cgtggcgatg gcggcggaaa gggcccggga tagcaccgga
     6721 gccccgggat ctacccaggg gaagaagggc gccacggcgc cccagagccc ctcgaagatc
     6781 cccggggccc cgtagaggag gacaaaggat cccaccgcca aagacaccac caccggcacg
     6841 aagaccgacg tcacccggtc ggcgaaggcc tggatgggaa ccttggtgcc ctgggcctcg
     6901 gaaaccaggg cgatgatctg ggaaagaaaa gaggcccctc ccacccgggt cacccggatc
     6961 ttgagaaagc catcgacgtt caccgtggcc cccgtcacct cgtccccggg ggacttcacc
     7021 acgggaagcg gctcgcctga gatcatggcc tcatccacgc tggccacccc ttccacgatc
     7081 tcgccatctt gggggatctt ttccccgggg cgcaccacca gcaggtctcc ggggaccaga
     7141 tcctccacgg ggatctccac ttccccttcc gggcgctcta ctcgggcggt gcgggccccc
     7201 atctccacca gcttggcgat ctcccgggtt gcccggctcc gggcccctgt ctccagatag
     7261 cgccccgtga ggtgaaaggc catgatcatg cctcccacca tggcgaagct cgtcacgggc
     7321 caccccgcca atttcaagag acccgaggca aaggaggccc ccgtacccag ggcgataagg
     7381 gtgtccatgc tggtatgccc ccgcaccccc tggcgcaccg cccggagaat ggtatcccct
     7441 ccggcgacga agatcaccag aaagctcaaa aactgctcca ataacaggtg ctgggtcaga
     7501 tcggagggga cgttgatgcc gaattcgtgc aggaccatga tcaccatcga agggaccgcc
     7561 aggatccagc cccagataaa gcgccttcgg gcctcgcgga gctcccgggc ggcgtagtct
     7621 tcggcttcgg ctcgcctctc tcggtggggt tccatacgaa aacccaattc ctgggccgcc
     7681 ctttccagcg ccccgggctc cacgaccccc gtcagatcca ccacggccct tttctgagga
     7741 aagttcaccg ccaccgagct cacggcggcc acctggcgaa ggcgcctttc caggttcatg
     7801 gcgcaggagg cgcagtgcat cccctccacg tcaaagacct tcctggagag ggaagggggc
     7861 gcctcctccc gcgccgaggc ggcaaagcca aggcgctcca ccgccttcac cagttcatcg
     7921 atcgaaacct cctccgaggc ctccaccgag gcccgacggg tcgccaggtt caccaccgcg
     7981 tccctcaccc ccgccacacc cttcaaggcc cgctccacgt tgcccacgca ggaggcgcag
     8041 tgcatcccct ccacgtcgag gacgattttg cgctcacctc gatgggaaga acctttgcct
     8101 tgatccacga ggatcccctc ccttcgaaaa attgataaat attttacctt gactgccaaa
     8161 aggcccttcc cgcagggcga gaaaaacctt cccgggaaag gggtcgcccc cggcgaaggg
     8221 cctcatcggt atatcacagg gggatccgga agggatccgt ataatttcgg tctttttgat
     8281 agccttactt ccccttatcc ccgaaggccc tggacactcc catcccgaag ccccataata
     8341 gcgatagata gggcgtttct ccaccaaaca tacggacccc gcccctcggc cgggcggcgc
     8401 agggaggaag gatcatgcga gagaacctac tttttgtgga cgatctccac gtgacgcgag
     8461 aagggaaccc catcctcaaa ggagtgacgc tccgggtcaa ccccggagag atcgtgggag
     8521 tgctggggcg caacggggcg ggcaagtcga gcctggccta cgccctcatg ggcacccatc
     8581 gctacacccc gtcgtcgggg aagatcttct ttgccggaga ggatatcacc ggagagccga
     8641 tctctcagcg ggccaagcga ggactaactt tagcgtggca acaccccgcc cggtacgagg
     8701 gtatcaccgt gcgccagtac ctccggctct cggcccaggg ggtcaaagag gcggacatgg
     8761 aggacgccct ctcccgggtg cagctcgaaa ccccctatct ggagcgcatg gtggacaagg
     8821 ctctctcggg gggggagcgc aagcgcatcg agctcgcctc catcttcctc atgcagcccc
     8881 ggctggccat gctggacgag cccgactccg gcgtggacct catcgccctg ggggacatca
     8941 tgcacctctt ccgcgacctg gcgggaggta ccaacggcgt actgctcatc acccaccggg
     9001 aggacgtggc ggcggcctgt gaccgttcct atctcatgtg caacgggcgc atcatcctgg
     9061 agggcagcgc cgaagccgta aagcgatact tcatgagtca gtgctctcct tgcaccgaaa
     9121 cgcccctcct tccggtcgct ccggaactcc cggccgcctt acccgtcccc aaggaggtgt
     9181 gttagccatg ttcagcgtgc aggacgaata caagaccctg gtggacatcg ccgaacgcac
     9241 cgcaggagaa ggctccggga gccttctggg acaggacgac gcggtgtacg tggtggtcca
     9301 cgccaacaag gtcttggccc accacgaact ccccggggtg accatcgagg ccgatgagct
     9361 gccagacggc atcgccgccc gcatcaccgt ggccgatggc accgtcatcg ccaagcccgt
     9421 ccatctgtgc ttcgggcacc tgggccatga ggggcgccag accatccaga gccgcgtcca
     9481 catcggcaaa ggagcccgag ccacctttct ggcccactgc atctttccca acgccatcga
     9541 gtttctccac gccatggaag gggacctcgt ggtggacgag gggggagatt tcacctacaa
     9601 cgaagtgcac gtccacggcc ccgaggggaa aatcaccgtc cgccccgtga cccgggtaac
     9661 gctggaaagg ggagccatct accgagggga cttcaccctg gtggagggac gggtggggga
     9721 cctggacatc cacatcgacg tggaagcccg gggagagcgg agccgggtgg agatcacctc
     9781 caaggtctac ggcaaggacg acgaccagtg tcaggtgcgg gacgtggtgc gcctttcggg
     9841 cgaggggagc accgccctca tcaaagcccg ggtggtgctt cgggaccgga gcgaagggcg
     9901 ttttctgggc ctcatcgaag gaggggccgc cggagcccgg gggcacgtgg actg
//
LOCUS       DAOOER010000065         9712 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_7460_length_9712_cov_8.150875, whole genome shotgun sequence.
ACCESSION   DAOOER010000065 DAOOER010000000
VERSION     DAOOER010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 9712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9712
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7460_length_9712_cov_8.150875"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PL162_07885"
     CDS             complement(<1..339)
                     /locus_tag="PL162_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HQF91973.1"
                     /translation="MENVLDLKALFHLSYGIYIVGSRRDGVYNGQIANAVMQITADPI
                     CVATCLHKNNLTTEFVRDSGVFSVSVLGQDVPMTFIGQFGFKCGRDLDKFCNVQHREG
                     PTGAPYVLDWT"
     gene            complement(396..884)
                     /locus_tag="PL162_07890"
     CDS             complement(396..884)
                     /locus_tag="PL162_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HQF91974.1"
                     /translation="MLDQKLYKELNDQINAELHSSYLYLSMASWFESENLKGMAKWMH
                     AQAEEEKVHAMKFYHFIEERRAKVELTAIEGPKTSWNSPLHAFEDAFAHEEYISGRIN
                     KLMTLAREVRDYATEELLHWFVKEQVEEEASVDEVVQKLKMLGDARNGLLMLDKELGK
                     RD"
     gene            complement(975..2375)
                     /locus_tag="PL162_07895"
     CDS             complement(975..2375)
                     /locus_tag="PL162_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HQF91975.1"
                     /translation="MTTDYDVIVVGAGPAGLFASQELTSQGYRVALMDKGRPLDARGC
                     PLKEGRSSRCLHCRPCGVICGWGGAGAFSDGKLTLTPEFGGNLERYVGRQRLLSLLEE
                     VDALWLKYGADAPVFEPEASFAGDVVRRGRVAGLQIIPARIRHIGTDKSKVVLGHLYE
                     ELATRCDIFLGTEVASVLVEGGRARGIRLASGKELRSRGVLVAPGREGAPWMERLARE
                     VNLEVDALPVDIGVRVEIPREWADPLTEEFYEVKALFNAPTFDDRVRTFCMCPGGEVV
                     TEYQSDSQVLVVNGHSNRDTKTENTNFALLVSTEFTKPFHDPNGYGTHIARLANMLGG
                     GVLVQRLGDLKAGRRSTEERIRRGLVRPTLKDAEPGDLAFVLPHRHLTDLLEMIRALD
                     SIIPGIDGPHTLLYGVEVKFYSLRLALDESLQSSIPGFFAAGDGAGVTRGVIQASASG
                     LVAARSLKTYIEGMGA"
     gene            2721..3488
                     /locus_tag="PL162_07900"
     CDS             2721..3488
                     /locus_tag="PL162_07900"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HQF91976.1"
                     /translation="MSDFQESALELAHLLSQSRRVVAFTGAGISTAAGLPDFRGPDGI
                     YQRAGLENPEEIFDIETFRRDPSIFYKFHREFLRILKDVEPTFTHRFLAQEERRGRLQ
                     GVVTQNIDALHQRGGSRRVLEVHGSVWGSHCTRCRTTYDYQISVVKTLREEIPRCESC
                     RGVLKPDVVFFGEAVNHMEESERLAEEADLMLVLGTALAVRPAAMIPALCPGKIVVVN
                     KGELFSHDLPRSRVHLFVDGELDPFFQAVASHMEPST"
     gene            3653..4558
                     /locus_tag="PL162_07905"
     CDS             3653..4558
                     /locus_tag="PL162_07905"
                     /inference="COORDINATES: protein motif:HMM:NF027852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgT C-terminal domain-containing protein"
                     /protein_id="HQF91977.1"
                     /translation="MKKSLYIAWALGWAVISLVAPLGTPAAAHTTPRSGMTVVVFPVR
                     NATDQGVWESKYYPGDILPDKLGKFFRQILKDAPLVEVREGDREPWLSGQRREGDLAV
                     DLEIYRFEPRRREGLGTAERGIVSLRMVVYDGTTGQERYRTVIDGKSSRWTPEFRQKP
                     DDPALIWASFEKSPFWIAFQNAARRARDDIFRGYTGYAVVGRLVSPMADSTRERPRYI
                     VSLGKFDSLKVGDLLAVGRSDTYITVDPENPVVVMPRLVGKVRVDFLKPREAAVTVVE
                     EDENDPIQLRDLVIMPLFSPRKGEW"
     gene            4559..5263
                     /locus_tag="PL162_07910"
     CDS             4559..5263
                     /locus_tag="PL162_07910"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQF91978.1"
                     /translation="MRSVALLLMAGGALLWTAVPLNAQGSSAVTEAAEAAPLSQERVD
                     HRDLWRRSEFLVATGRYSQARELLQRMLAEDRENPHVWYLLGKVAELRGDLEEAQVAY
                     GQTLALAPGYPELSRVLMAHSRGDALPIWDPRRQKKSLPKAAPAAQNPIPPRDGASRH
                     AVALGVEIPLLVGPEAKMERLQAPQPMAPPAPVVPPSGAIVPWTGAPSPPPKEETLSM
                     DRPGSPSPRPRPDERP"
     gene            complement(5447..7189)
                     /locus_tag="PL162_07915"
     CDS             complement(5447..7189)
                     /locus_tag="PL162_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03768 family metallophosphoesterase"
                     /protein_id="HQF91979.1"
                     /translation="MVLKKPGYRTTVLGVLLLVLSAFLSSGCGSSESSRGGVPSGYPI
                     DSRVATTVVRTVVPDEIYQSADKIFPYEVPRYAENGYGAWHYGPGAPLTKRLDLMPTG
                     YQDGAAVPGGRLLHFFTITDVHISDKESPSQAIYYGYKGGLSSAYSPVMLCTTQVLDA
                     VVQTANALHGQKPFDFALSLGDICNNTQYNELRWYIDVLDGGAINPDSGVKDDPVPGP
                     LNDYQDPYRAPGLIPSIPWYQTLGNHDHFWIGSSPVNDYLRQIYTGDTVMALGTWQEV
                     FYLPEGVTKRDYYMGAIDGRTVDGTVFGAGASRDFATAPKIPAADGNRRSLLRREWLA
                     EFFKTTGNPVGHGFPRSSIETGFACYSFEPRPDVPLKVIVLDDTQREDDVFDQGYGHG
                     SLDKERYDWLVKELDEGQAQGKLMVIAAHIPIGVCPPGALDGWWAKAYISEDQLFATL
                     HKYPNLLLWLAGHRHVNAITAFPSPDPNHPELGFWQVETASLRDFPQQFRTFEVVCNS
                     DRTVSVLATAVDPAVREGTPAALSRTYGVAAQQLFKNDLPYLPTGVSNAELVKPLSPA
                     MQAKLAPWSDLQGK"
     gene            complement(7476..8777)
                     /locus_tag="PL162_07920"
     CDS             complement(7476..8777)
                     /locus_tag="PL162_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HQF91980.1"
                     /translation="MRDWLENRFQIKAAGSDIKTEVLAGVTTFMTMAYIIFVNPNILS
                     NAGMPLGPLMVATCLASSLATFCMAFLANYPIALAPGMGLNAFFAFGVVLGMGLSWQV
                     ALAAIFVEGVLFIILTMTKLRESVVNDIPKSLKIGISTGIGFFIVFIGLQNAGIIVRN
                     DAVLVGLGNLKSPPVLLAFLGFFLMVALEARKIRGSILWGILTVSAVAMALGVVKTPE
                     AIVSLPPSLGPIFMKMDFSGLMSPNFWIVMFSFFFVDFFDTVGTLVGVTSRVGMLDAQ
                     GRLPRAREALLSDAIGTVAGAMLGTSTVTSYVESASGVEQGGRTGLTALVTAVLFMGA
                     LFFSPLVAVVPSCATAPALVMVGIFMMMGLKELDFGDWTDLVPACVAIFVMPLSYSIA
                     HGIEFGIVSFVVLKIITGRHRDVSPLMVGLALLFVAKEAFF"
     gene            complement(9005..>9712)
                     /locus_tag="PL162_07925"
     CDS             complement(9005..>9712)
                     /locus_tag="PL162_07925"
                     /inference="COORDINATES: protein motif:HMM:NF024774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HQF91981.1"
                     /translation="RVWKGGRLVVTDGRLVGGPVPRMIPPKPLGEVVSVPEEGAFRLP
                     VPPGARIRAMGLRPGQVVTDHLVLTPSMRDGEAVADPARDLALMMVLEKNRGTGRHAL
                     GFVRGLGLQEGALGSSVAHDAHNFVAVGMDGLSMRTALKALVSAGGGIVATRGDRVEA
                     FLELPVGGLMNPGSPGDVARGLERVEDAATRLGTSLPHPFMALSFLSLSVIPELKLTD
                     QGYVDLGRGGIQTLFVE"
BASE COUNT         1915 a         3192 c         2868 g         1737 t
ORIGIN      
        1 cgtccaatcc agcacatagg gggcccccgt gggaccctcc cggtgctgca cgttgcagaa
       61 cttgtccagg tctcggccgc acttgaagcc aaactgaccg atgaaggtca tgggcacgtc
      121 ctgccccagc accgatacgg aaaaaacgcc gctgtcgcgg acaaactccg tcgtgaggtt
      181 gtttttgtgg agacaggtag ccacacatat ggggtccgcc gtgatctgca tcaccgcatt
      241 ggcgatctgt ccattgtaga cgccatcgcg gcgtgagccc acgatgtaga tcccgtagct
      301 caggtgaaag agtgccttaa ggtctaagac gttctccatg ggggcctcct cgctctttca
      361 ttaagaagct aaaaactgcg cggccgcggc aaagtctaat cccgcttccc cagctccttg
      421 tcgagcatga gaaggccgtt ccgggcgtca cctagcatct tgagcttctg caccacctcg
      481 tccaccgagg cctcttcctc cacctgttcc ttcacaaacc agtgcaggag ctcttccgtc
      541 gcgtagtccc gcacctctcg ggccaacgtc atgagcttat tgatccgccc cgagatatac
      601 tcttcgtggg caaaggcgtc ctcgaaggca tgcagcggag agttccagga tgtcttggga
      661 ccctcgatgg cggtgagctc caccttggcc cggcgctctt cgatgaagtg atagaacttc
      721 atggcgtgca ctttttcttc ttctgcctgg gcatgcatcc acttcgccat gcccttcagg
      781 ttctcggact caaaccaaga ggccatagac aggtacagat aggacgagtg gagctccgca
      841 ttgatctggt cgttcaactc tttgtaaagc ttctggtcta gcattaaaaa cacctcccta
      901 cagaatggcg agccccagga gctcgctaaa caaaaacgct ttcagaaaag attttacccc
      961 tcctgccctc cgtgtcaagc ccccatcccc tcgatgtagg tcttaagcga ccgagccgcc
     1021 acaagaccgc tggcggaggc ctggatgacg ccccgggtca cccctgcccc gtctccggcg
     1081 gcgaaaaagc cgggaatgga gctttgaagg ctttcgtcca acgccaggcg caggctgtag
     1141 aacttgacct ccacgccgta aaggagggtg tggggtccgt cgatgccggg gatgatgcta
     1201 tccagggctc ggatcatctc cagtaggtcc gtcagatggc ggtggggaag cacgaaggcc
     1261 aggtctcccg gctccgcgtc cttgagcgtg gggcgcacga ggccacggcg gatccgctcc
     1321 tcggtggagc gacggccagc cttgaggtcg ccgaggcgtt gcacgaggac ccctcccccc
     1381 agcatgttcg ccaaacgggc gatgtgggtg ccgtagccgt tggggtcgtg aaaaggtttt
     1441 gtaaactccg tgctcaccag cagggcaaaa ttggtgttct ccgtcttggt gtcccggttg
     1501 ctgtggccat tcaccaccaa cacttggctg tcgctctggt actccgtcac cacctctcct
     1561 ccggggcaca tgcaaaaggt ccgcacccga tcgtcaaagg tgggagcgtt gaaaagagcc
     1621 ttgacctcgt agaactcctc ggtgagggga tcggcccact cccggggaat ttccacccgc
     1681 acgccgatgt ccaccggaag ggcgtccact tccaggttta cctcccgggc caggcgctcc
     1741 atccaggggg ccccctcgcg tcccggggcc accaggactc cccggctccg gagctctttg
     1801 ccactggcca ggcgaattcc ccgggccctc cccccttcca ccagcaccga ggccacttcg
     1861 gtccccagga aaatgtcgca ccgtgtcgcc agctcttcgt ataggtgccc caggacgacc
     1921 ttggacttgt ccgtgccgat gtgacggatc cgggccggga tgatctggag ccccgccacc
     1981 cgcccccgcc ggaccacgtc ccccgcaaag gaggcctcgg gctcgaagac cggagcatcg
     2041 gcgccgtact tcagccacag ggcatccact tcctccagaa gggacaaaag cctttgtcgc
     2101 cccacgtaac gctccaggtt tcctccgaac tccggggtaa gggtcaactt gccatcgctg
     2161 aaggcccccg ccccgcccca accgcagatg acaccgcagg gacggcagtg gaggcagcgg
     2221 gaagaccgac cctctttgag ggggcatccc cgggcgtcca aggggcgccc cttgtccatc
     2281 agggccaccc gatacccctg ggaagtcaac tcttgggagg caaaaagccc cgccggcccc
     2341 gctccgacca caataacatc gtaatctgtc gtcattttct cctccttgcc accgccgtac
     2401 tcctcaggag gaaggctccc gaagctcccg cccattgtag catggaaagg atcgtctatt
     2461 cccccggacg ggaaagccca tctttcgaaa ggcccgtccc cttcgcccac caccgcccat
     2521 ttcccggacg agggcccccg gaaggccggg aaagcgggga aggactcttg aggatcgccg
     2581 aaagaggctt caccgaaagg gtgattttgg ggtatcttag gggaagtcgt cgtcactcca
     2641 tccccgaggg accgacgccc ccagaggagc gaccccgcag ggggaagcct ctttcgtgag
     2701 agagaaagga ggacgttacc atgtctgact tccaggaaag cgcgctcgaa ctcgcccacc
     2761 ttctgtccca atcccgccgg gtggtggcct tcaccggagc cgggatctcc accgccgccg
     2821 ggcttcccga ttttaggggc cccgatggaa tctaccagag ggcgggcctg gaaaatcccg
     2881 aagaaatttt cgacatcgag accttccgga gagatccttc gattttttac aagtttcata
     2941 gggagttcct ccggatcctg aaggatgtgg aacccacgtt cacccaccga tttttggccc
     3001 aggaagagcg gcgggggcga ctccagggcg tggtcaccca gaatatcgac gccctgcacc
     3061 aacggggggg ctcccgccgg gtgctcgaag tccacggcag cgtctggggc tcgcactgca
     3121 cccgatgccg caccacctac gactaccaga tcagcgtggt caagactctt cgagaagaaa
     3181 ttccccgttg tgagtcctgt cgcggtgtcc taaagcccga cgtggtcttt tttggagagg
     3241 cggtgaatca catggaagaa agcgagcgac tggccgagga agcggatctc atgctggttc
     3301 tcggcaccgc cctcgccgtc aggcccgccg ccatgatccc cgccctctgt cccggaaaga
     3361 tcgtggtggt caacaaagga gagctcttct cccacgacct accccggtca cgcgtccatc
     3421 tcttcgtgga cggggagctc gacccctttt ttcaggccgt ggcctcgcac atggagcctt
     3481 cgacgtagcc ctaagaccgg aaggtcgacc tcggggcctc caggtggtcc tctccgaaaa
     3541 atcgccagcc cccctgcccc tcggaggggt cttgaccttt cccttttccg ccgatactgc
     3601 caatgcgggg caaggccgca caaaaaggac ccatcggaag gagatttttt ccatgaaaaa
     3661 aagcctctat attgcctggg ccctggggtg ggccgtgatc tctctcgtcg cccccctggg
     3721 gacgccagcg gcggcccata cgaccccccg atcggggatg acggtggtgg tctttcccgt
     3781 acggaacgcg acggaccagg gggtctggga gagcaaatac taccccggag acatcctccc
     3841 ggacaaactg ggaaagttct ttcgccagat cctcaaagac gcccctctgg tggaggtgcg
     3901 ggaaggagat cgggaaccct ggctctcggg gcaaaggcgc gagggggatc tggcggtgga
     3961 tctggaaatc taccgcttcg agccccggcg ccgagaaggc ctgggcacgg cggaacgagg
     4021 catcgtatcc ctgcgcatgg tggtctatga tggcaccacg gggcaggagc gctatcgcac
     4081 ggtgatcgac gggaaaagct cgaggtggac tcccgagttc cgacaaaaac ccgacgatcc
     4141 ggccctgata tgggcctctt tcgagaaaag tcccttttgg atcgcctttc aaaacgcggc
     4201 ccgcagggcc cgggacgata tcttccgggg gtatacggga tacgcggtcg tgggtcgcct
     4261 ggtgtcgccc atggccgatt ccacccggga gcgccctcgg tacatcgtct ccttgggaaa
     4321 attcgactcg ctgaaggtgg gagatctcct cgccgtgggg cgaagcgata cctatatcac
     4381 cgtcgatccg gaaaaccccg tggtggtcat gccgcgtctg gtgggcaagg tgagggtgga
     4441 tttcctgaag cctcgagaag cggcggtgac cgtggtggaa gaggacgaaa acgaccccat
     4501 ccagctccga gatctggtca tcatgcctct tttttctccc agaaaggggg agtggtagat
     4561 gagatccgtc gctttgttgc tgatggctgg aggggcgctc ctgtggacgg ccgtcccttt
     4621 gaatgcccag ggatcctccg ccgtcaccga agccgcagaa gccgcgcccc tgtcccagga
     4681 aagggttgac caccgggacc tgtggcgccg gagtgaattc ctggtggcga ccggacggta
     4741 ctctcaggcc cgagagcttt tacagcgcat gttggcggag gaccgggaaa acccccacgt
     4801 ctggtacctc ctgggaaagg tggcggagct tcggggagac ctggaggaag cccaggtcgc
     4861 ctacggacaa acgctggccc tggccccggg gtaccccgag ctctcccggg tcctcatggc
     4921 ccactcccgg ggggacgccc tgcccatctg ggatccccga aggcaaaaaa aatccctgcc
     4981 caaggcggcg cctgcggccc aaaatcccat tcctccccgg gacggagcct ctaggcacgc
     5041 cgtggccctg ggggtggaga ttcccctcct cgtggggccg gaagccaaga tggagcgcct
     5101 tcaagccccc cagcccatgg cgccccccgc ccccgtcgtg cccccctccg gggccatcgt
     5161 gccctggacc ggggcgcctt cgcccccgcc taaagaggag accctttcga tggaccgccc
     5221 agggtctccc tccccccggc cccgaccgga cgaaaggccc taaggaccct tccgacttct
     5281 ggaaaaggtc ccccggaaga ctcaaaagac cccgggcaaa ggcctcttcg tcaacctttc
     5341 tgcgcccgcg acgacgcaca aagccggggc cccttcaggg ccccggctcg ctatttactt
     5401 tgccaccggt cctccctttc tccggggggc tcttgggcca tggggtttat tttccctgaa
     5461 gatcgctcca gggcgccagc ttggcctgca tggccgggct caggggtttg accagttccg
     5521 cgttggaaac ccccgtggga aggtagggga gatcgttttt aaaaagctgc tgagccgcca
     5581 cgccataggt gcgcgacagg gcggccgggg ttccttcccg aacggcggga tccacagcgg
     5641 tggccaagac cgagacggtg cggtcgctgt tacagaccac ttcgaaggtc ctaaactgct
     5701 gtggaaaatc cctcagggac gccgtctcca cctgccagaa accgagctcc ggatggttcg
     5761 ggtcggggga aggaaaggcg gtgatggcgt tgacatggcg atgtcccgcc agccaaagga
     5821 ggaggttcgg gtacttgtgg agcgtggcga aaagctggtc ctcggagata taagccttgg
     5881 cccaccaccc gtccagggcc cccggggggc acaccccgat gggaatatgg gccgcgatga
     5941 ccatgagctt tccttgggcc tgcccttcgt ccagctcctt taccagccag tcgtagcgct
     6001 ccttatccag ggagccgtgc ccgtagccct ggtcaaagac gtcgtcttcc ctctgggtgt
     6061 cgtccagtac gatgaccttg agggggacgt ccggccgggg ttcgaagcta tagcaggcaa
     6121 atcccgtttc gatggaggac cggggaaatc cgtgccccac ggggttcccc gtggttttga
     6181 agaactcggc cagccactcc ctcctcagga gggaacggcg gttcccgtcg gcggccggga
     6241 tcttcggggc ggtggcgaaa tcccgcgagg ctccggcgcc gaagaccgtg ccatccaccg
     6301 ttcggccatc gatggccccc atgtagtaat cccgcttagt gacgccctcg ggcaggtaaa
     6361 acacctcctg ccaggttccc agggccatca cggtgtctcc cgtgtagatc tgacggagat
     6421 aatcgttcac tggagaagac ccgatccaga agtgatcgtg gtttcccagg gtctggtacc
     6481 aggggatcga cgggataagc cccggagccc ggtaggggtc ctggtagtcg ttcaggggcc
     6541 ctggcaccgg gtcgtctttc accccggaat cggggttgat ggcgcctccg tcgaggacat
     6601 cgatatacca acggagttcg ttgtactggg tgttgttgca gatatccccc agggacaggg
     6661 caaagtcgaa gggcttctgg ccgtgtaggg cgttggccgt ttgcaccacg gcgtccaaga
     6721 cctgggtcgt gcacagcatc accggggagt aggccgaaga gaggccccct ttgtagccat
     6781 agtagatggc ctggctgggg gattctttgt cggagatgtg aacgtcggta atggtaaaga
     6841 agtggagcaa ccgccctcca gggacggcag ccccatcttg atatcccgtc ggcatgaggt
     6901 caaggcgctt ggtcaggggc gccccggggc cgtagtgcca cgccccatag ccgttttcag
     6961 cgtatctagg cacctcgtag gggaaaattt tgtccgcgct ctggtagatc tcatcgggaa
     7021 ccacggtccg aacgaccgtc gtcgccaccc gagagtcaat gggatacccc gaagggaccc
     7081 ctcccctaga tgattccgac gagccgcagc ccgacgaaag aaaggccgac agaaccaaca
     7141 gcaacactcc cagaacggtc gttcggtatc caggcttttt tagcaccacg atcttccctc
     7201 tttccaagac gcctcagatt ttttaagcgt aaaatgcctc cctccatggg atggcatccc
     7261 ctcccttccg tcgccagccc catgacaaag gaaccccatc gccgccacgc cctccgaaac
     7321 cccccgaggg gccgaggcgg cccccaccga tctaaagatc gcccattcta tcacatgtcc
     7381 ggggcgtttt ccgacgcccc cttcgccccc cagacccctt ccaggcacaa aaatcacgga
     7441 aaaaacccag aggcttccta tatcccttca tgcctttaga aaaacgcttc cttggccacg
     7501 aagagcaagg ccagccccac catcagaggg ctcacgtccc gatgacgccc cgtgatgatc
     7561 ttaagcacca caaaggagac gatgccaaac tcgatgccgt gggcgatgct gtaggatagg
     7621 ggcatgacga agatcgccac gcaggcgggc accaggtcgg tccaatcgcc gaaatccagc
     7681 tccttcaatc ccatcatcat gaagatgccc accattacca gcgcaggagc cgtggcgcag
     7741 gagggcacca ccgccaccag aggggagaag aacagggccc ccatgaagag cacggcggtg
     7801 accagggccg tgagccccgt ccgccccccc tgctccacgc cgctggcgct ttcgacatag
     7861 gaagtcaccg tggaggtccc caacatcgcc ccggccaccg tgccgatggc atcggaaagc
     7921 agggcctccc gggcccgggg aaggcgtccc tgggcgtcca gcattcccac ccgactggtg
     7981 actcccacca gggtccccac ggtgtcaaag aagtccacga agaagaacga aaacatgacg
     8041 atccagaagt tcggactcat caggccggaa aagtccatct tcatgaagat gggtcccagg
     8101 gacgggggca gggagacgat ggcctcgggg gtcttcacca cccccagcgc catggccacc
     8161 gccgacaccg tcaggatgcc ccagaggatg gagcctcgaa tcttacgggc ttccaacgcc
     8221 accataagga agaaccccaa aaaagccagg agcaccggag ggcttttgag gtttcccagc
     8281 cccacgagga cggcgtcatt tcgcacgatg atcccggcgt tctgaaggcc gatgaagacg
     8341 atgaaaaaac ctattcccgt ggaaatgccg atcttcaggg acttggggat gtcatttacc
     8401 acgctctccc ggagcttggt catggtgagg atgatgaaaa gcaccccctc cacgaagatc
     8461 gccgctaggg ccacctgcca ggagagcccc atgccgagca ccacgccgaa ggcgaaaaag
     8521 gcgttgagcc ccataccggg ggccagggcg atgggatagt tggccagaaa ggccatgcaa
     8581 aaggtggcca gggagctcgc cagacaggtg gccaccatga gggggcccag aggcatgccc
     8641 gcgtttgaca ggatgttcgg gttcacaaag atgatgtacg ccatggtcat aaacgtggtg
     8701 actcccgcta aaacttcggt ttttatgtca ctgcccgccg ccttgatctg gaaacggttt
     8761 tcgagccaat ctcgcatggc cattcctcct ttttggattt tttccggaac tcctccggac
     8821 ttcgcccctt cgtgggcgct cccatggcca aaacaccgaa ataaaacgtc tcgccgtccc
     8881 ccgggtgccc ggggagtttc gccgaaggcc ccgattcctc cggcgccctc ccccgcagcg
     8941 cccctccccc gtcgtcatgc cccccgaaga caaccgaggc atggggcccg gtgggggcct
     9001 ttcctcattc cacgaagagc gtttggatcc ctccgcgccc cagatccacg tatccctggt
     9061 cggtgagctt cagctcgggg atcaccgaaa gagataggaa actcaacgcc atgaaaggat
     9121 ggggcaggga ggtccccagg cgggtggcgg cgtcttcgac cctctccagc ccccgggcca
     9181 cgtcccccgg agatcccggg ttcatgagtc cccccacagg aagctccagg aaggcctcca
     9241 cgcgatctcc ccgggtggcc acgatccctc cgcccgcaga aaccagggct ttcaaggccg
     9301 tccgcatcga caagccgtcc attcccaccg ccacgaagtt gtgggcgtcg tgggccaccg
     9361 aagatcccag agccccctcc tggagcccca atcccctcac gaaccccagg gcgtgacgcc
     9421 ccgtgccccg atttttctcc agcaccatca tgagggccag atctcgcgct gggtccgcca
     9481 cggcctctcc gtcccgcatg gagggcgtca ggaccagatg atccgtcacc acctgtcccg
     9541 gccttaggcc catcgcccgg atccgggccc ccggaggtac cggaaggcga aaggctcctt
     9601 cctcgggcac cgaaaccact tcccccaaag gtttgggagg gatcatacga ggcacgggcc
     9661 cccccacgag gcgcccatcg gtgaccacca gacggccgcc cttccaaacc cg
//
LOCUS       DAOOER010000066         9533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_7625_length_9533_cov_6.361996, whole genome shotgun sequence.
ACCESSION   DAOOER010000066 DAOOER010000000
VERSION     DAOOER010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 9533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9533
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7625_length_9533_cov_6.361996"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="PL162_07930"
     CDS             <1..770
                     /locus_tag="PL162_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HQF91982.1"
                     /translation="IFLTETAQLADVVLPAACWAEKDGTFTNTMRAVQRVRQAVRAPG
                     QAKADWEILLELGKRCGAPWDFSSPSEIFDDVARFVPSYAGISYARIEETPISWPCPA
                     PDHPGTPILHGAKFARPNGKGFFTPCEWQAPHEWPDEAYPFLASTGRLLYHYHTMSMT
                     RRSAPGEFISELFIEINPEDASSLAVCPGDLLRVTSRRGTVEGKAKITDRVPPMMVFV
                     PFHFGETPANALTAAAIDPTSETPAYKISAVRVEKAS"
     gene            930..1595
                     /locus_tag="PL162_07935"
     CDS             930..1595
                     /locus_tag="PL162_07935"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HQF91983.1"
                     /translation="MDNSRHILPVSAVILAGGRGKRIGGNKLFLHVEGQAVLEVLLRR
                     LSGWMAEMLLVVSPGEERAAEELLEKLHRREAVRVVQDRHGGLGPLAGLVRGMEAAQY
                     DWIFALGCDMPDVHEAVARILWLRRLHLEAPWREYEAVLARIGGYLEPLHAFYRSPCR
                     EVFQGLLAQGRRKIGDGFPEIAAAVVEEEDLSVIPGYRASFRNLNTSSQLAAWLRERR
                     ERE"
     gene            1948..2469
                     /locus_tag="PL162_07940"
     CDS             1948..2469
                     /locus_tag="PL162_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HQF91984.1"
                     /translation="MTGCCSGTDKRFSELESFINALPDKKGELVTVLHKAQGIFGYLP
                     REVQEFVADKMDLSVAKVYGVVSFYSFFTMQPKGKYAINCCLGTACYVRGAEDIVAEL
                     EKELHIKAGQVTPDGRFSIDTLRCVGACGLAPVIIINGKVYGRVKASDIPGILDEYRH
                     DVEDPSKAEVCHG"
     gene            2462..2866
                     /locus_tag="PL162_07945"
     CDS             2462..2866
                     /locus_tag="PL162_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HQF91985.1"
                     /translation="MASKIGSLEELKKLREQMQNATALREKGQNIEKLVEVKVSMATC
                     GIASGARETMADMMEEARRRHMENIVFTQSGCMGYCHSEPTIVIVRPGEEPVTFGNVK
                     GADRIREIFDKFIERGELVDGFIPAAYRTIHE"
     gene            2902..4695
                     /locus_tag="PL162_07950"
     CDS             2902..4695
                     /locus_tag="PL162_07950"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012868905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HQF91986.1"
                     /translation="MADMKMQLLVCGGTGCVSARSNEIVAALRAALKDQGLENDVAVV
                     LSGCFGFCEKGPIVKVAPDNTFYVQVTPEDAGEIVAEHIVKGRKVERLLYVDPETNNR
                     VSDSKHMDFYKKQMRIALRNCGFVDPENIGEYIARDGYQALGTVLSTMTPAQAVDVMK
                     KSGLRGRGGGGFPTGLKWEFASKFSADQKYVICNADEGDPGAFMDRSILEGDPHSVIE
                     AMIICGYAINATKGMVYIRAEYPLAVQRLHKAIDDAREMGLLGDHILGSDFSFDIELR
                     YGAGAFVCGEETGLIRSLEGERGEPTSKPPFPAESGFWGKPSNVNNVETFACVPVIYQ
                     KGAEWFSKIGTEKSKGTKVFALAGKVNNVGLVEVPMGTTLREVIFDIGGGIRDGKAFK
                     AVQTGGPSGGCLTTKHLDTPIDFDNLVAAGSMMGSGGMIVIDEDNCMVSLARFYLEFT
                     VEESCGKCTPCRVGNKRLHEILTKICQGRGTEEDLALLRDLGKMIKDTSLCGLGQTAP
                     NPVLSTLEHFWDEYVAHVRDKRCPAGMCKDLVQIVIDPEKCKGCTMCAKVCPAGAISG
                     KAREIHVIDQEKCIKCGACIGVCKFDAISKQ"
     gene            4715..6472
                     /locus_tag="PL162_07955"
     CDS             4715..6472
                     /locus_tag="PL162_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006584326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HQF91987.1"
                     /translation="MISLNIDGKYVKVPEGSTILTAARALGIDIPTLCHLHLHGTGMV
                     NKAASCRICVVEVKGRRNLAPACATPVVEGMEVRTNTIRVMNARKMVMELLLSDHPKE
                     CLTCAKSGSCELQELAERFCLRESRFEGGDQSTYAHDRSAAIERDMDKCVMCRRCETM
                     CNEVQTVGALSGINRGFKSVVAPAFEMPLEESVCTFCGQCTAVCPTGALVERDYSWKV
                     FEALADPDKVVVVQTAPAVRAALGEAFGMPPGTLVTGKMVAALRAMGFDHVFDTDFAA
                     DLTIMEEGSELLDRLTRFLEGDTSVRLPILTSCCPAWVKFFEHKFQDMLDVPSTAKSP
                     QQMFGAIAKTYFAGKLGIPREKMVVVSVMPCLAKKYECSRPEFFVNGNPDVDLSISTR
                     ELAHMIKRANIDFVNLPDEDFDGPMGESTGAGVIFGTTGGVIEAATRTAVEIYTKKPL
                     DSVDFLALRGFDGCREARVKVGDLELRIGIAHGLGNARHLLESIRKGEVEPFHAIEIM
                     ACPGGCVGGGGQPYHHGNMNIVRKRAEAIYREDAAKPLRKSHENPEIVRLYEEFLGKP
                     LGEKSHHLLHTHYFKRHKV"
     gene            complement(6613..6969)
                     /locus_tag="PL162_07960"
     CDS             complement(6613..6969)
                     /locus_tag="PL162_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91988.1"
                     /translation="MTKKGVWNGILLIMVLWGTMMTPLPGEGVVSPSEVEVLLVDLIL
                     VPPEIVGMRDMKHERFSWDRNLTVFLDHEGQKMDAEAFLRRYDRQVVVLVRSPSGEIT
                     EAYPKGIKAGGGQESP"
     gene            complement(6972..>9533)
                     /locus_tag="PL162_07965"
     CDS             complement(6972..>9533)
                     /locus_tag="PL162_07965"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HQF91989.1"
                     /translation="YDFFKNSTRSPVVSYCQDNWLVVCCDGNDDTKGKGLGEDPALAV
                     KNLYDNTGKLYVGKKLEDMNYPVRTMVIGFVDPAKEPTLAATLNKMADYGDDGKLNDS
                     AQAYFARDTSELVRAFQDVILRVRQVSGSPSTPVVSPPRMDPQDEGAAYVPTFTTEPS
                     DQWQGHLYKFGMDAAGNLTGKTIWEAGAKLQARSWSDRRVYTANWSASLPRDPGGSNL
                     VRFSEGNAASLAPEIWCNVDGAADLEGKIKPLIRWTLGKNEFAKPGSPPERWKLADIN
                     RSGLFVVRKPWGTYPDASYSAFAWALRSRDARVYVQSNGGMLHAFEARSRDADGRALN
                     HGEERWAFIPPQILAGRRILGTKVLLGSNPLDPKGGLSPSWITDGTSVPRHLLDGPLA
                     VKDVRLKLDGKWSYRTVLLGCLGFGGKGLYALDITSPDTPRFLWAVDNANYENSGLAT
                     ATPQPLHQVLWWKSLLAEEKEDGGGSGTSAMLHAFTHRTYTRASKKDPVIVDVSANLE
                     AASPDFSLVSAYRRLGMTTSVPFIGRIPLGGEYPFVALFGGGLTGEKKGVPTTEGRGL
                     FVVDIQSGRVLRLVSEDRWGTPLDMVSSPVYPYTSRSDGIWEGVFIPDAAGNIFKAWG
                     KGLVSVDQIFRLDPNKSVAINQGLGAGIINGEIWLYGGTGNPEGVWNRDYNAKNYIFG
                     VNAGGFEAAPLKKDLSHLQKLSSKDVGSTSSDPDGWYFDLTPGEYLSAAPAFTHRSAG
                     SFVALATFCPTLDRCGTGGIGRLYIVNAKSGRGAWNSNGKKVVEISGMKISSVSFSGS
                     KLYLTGIFSATGTNMGDGDIKGLTKNEGIAVMDIPSGMGAGDDSDSSQEVLYWRD"
BASE COUNT         1903 a         2873 c         2910 g         1847 t
ORIGIN      
        1 acatctttct cactgagacg gcccaattgg ccgatgtggt ccttcccgcc gcctgttggg
       61 cggaaaaaga cggcacgttc accaacacca tgcgggcggt ccagagggtt cgtcaggccg
      121 tccgggctcc ggggcaggcg aaggcggatt gggaaatcct cctggagctg ggaaaacgct
      181 gcggagcccc ttgggacttt tcttctccct cggagatctt tgacgatgtc gcccggtttg
      241 tgcccagcta tgcgggcatc agctacgccc gcatcgagga gacccccatc tcgtggccct
      301 gccccgcgcc ggatcacccc ggcaccccga ttctccacgg ggccaaattt gcccgcccta
      361 acggcaaggg atttttcacg ccgtgcgagt ggcaagcccc ccatgaatgg cccgacgaag
      421 cctatccctt cctggctagc acgggacgct tgctctacca ctatcacacc atgagcatga
      481 ctcggcgcag cgctcccggg gagttcatct cggaactatt catcgagatc aaccccgaag
      541 acgcctcgtc cctggcggtg tgtcccggag acctcctgag ggtgacttcc cgccgaggca
      601 cggtggaggg aaaggccaag atcaccgacc gggttccccc catgatggtc tttgtgccgt
      661 tccacttcgg cgagacccct gctaacgccc tcaccgccgc cgccatcgat ccgacttcgg
      721 agactccggc gtacaagatc agcgccgttc gggtggaaaa agcctcctga tccctactct
      781 taggcggctc caaggaaact caggcgaagc acccctccgg agcgatgggt gcttcgcttt
      841 tttttcggat gacgtatact acgaggcgac gggcgaaggg caagcaaact ccccccggag
      901 agttttcgtc atgaagggag aatggattgg tggacaactc tcgacacatc ctgcccgtgt
      961 ccgccgtgat attggccgga ggcaggggaa agaggattgg gggaaacaag ctttttctcc
     1021 acgtcgaagg acaggcggtc ttggaggtgc tcctgcggcg cctttcgggg tggatggccg
     1081 agatgctcct ggtggtctct ccgggagagg agcgcgcggc ggaagagctc ctggaaaagc
     1141 tccatcgccg cgaggccgtt cgcgtcgtcc aggaccggca cgggggtttg gggcctctgg
     1201 cggggctcgt caggggtatg gaagcagccc aatacgactg gatctttgcc ctgggatgcg
     1261 acatgcccga cgtacacgag gcggtggccc gaatcctatg gctccggcgt cttcacctcg
     1321 aagccccttg gcgggaatat gaggcagtgt tggcgcgtat cggcggttat ctggaaccat
     1381 tgcatgcctt ttatcgctcc ccgtgtcgag aggtttttca ggggcttctt gcccagggaa
     1441 gacggaagat cggcgacgga ttcccggaga tcgccgccgc cgtggtggag gaagaggact
     1501 tgtcggttat cccaggatat cgagcctcgt ttagaaatct taacacttcg tcgcagctgg
     1561 cggcttggct tagggagcgg agggagcgag agtagcgaga gtgaaatggc gaggcgcccc
     1621 ggagagaaag gccagatgcc cgagggcgtc ccccagatcc agcgctctgg gcttgttcgg
     1681 gcaagggtaa cgccccgggt tccgaggacg aaagaagcaa aaacaagcta aaataacagt
     1741 gatatattac atacttgaca aaggcccctt ctcccggtag agtgcggggg aatctgctcc
     1801 gtgaagagaa tcactttctc ctcagggctt aaagaactgg tcttgcggat tcaagacccc
     1861 ggggcttccg aaggaaagat gccgggggtg cggtgtggcg ggaggtctcc ggggaaatgc
     1921 ttcagatttg attaaggagg cttggaaatg acgggttgct gcagtggaac ggacaaacgt
     1981 ttctcggaat tggagtcttt catcaacgcc ctcccggata aaaagggaga gttggtgacc
     2041 gtgctccaca aggcccaggg aattttcgga tatcttcccc gggaagtgca ggagtttgtg
     2101 gcagataaga tggacctctc cgtggccaag gtctacggcg tggtgtcgtt ttattcgttt
     2161 tttaccatgc agcctaaagg aaagtatgcc attaactgct gcctgggcac ggcctgctac
     2221 gtgcgggggg ccgaagacat cgtggctgag ctggaaaaag agctacatat caaggcggga
     2281 caggtgacgc cggacggccg tttttccatc gacaccctgc gatgcgtggg agcctgtggc
     2341 ctggcccccg tcatcatcat caacggcaag gtctacggtc gggtaaaggc ttctgacatc
     2401 ccgggcatcc tggatgaata ccggcacgac gtcgaagacc catccaaagc ggaggtgtgc
     2461 catggctagc aagatcggat ctttggaaga gcttaaaaaa cttcgtgagc agatgcaaaa
     2521 cgccacggcc ctgcgggaaa aggggcagaa catcgagaag cttgtcgaag tcaaggtgag
     2581 catggccacc tgcggcattg cctcgggggc ccgggaaacc atggcagaca tgatggaaga
     2641 ggcccgtcgt cgccatatgg agaacatcgt cttcacccag tccggttgca tggggtactg
     2701 ccactcggaa cccaccatcg tcatcgtccg ccctggcgaa gaaccggtga cctttggcaa
     2761 cgtcaagggt gcggatcgca tccgagagat ttttgacaag ttcattgagc ggggcgaatt
     2821 ggtggacggc ttcattcccg cagcgtaccg caccattcac gaatagcgcc gcctccgtag
     2881 ggaaaggagc gtttggaatc catggccgat atgaagatgc aacttctcgt gtgcggcggc
     2941 acggggtgcg tttccgcccg cagcaatgag atcgtcgcgg cgcttcgcgc cgccttgaag
     3001 gatcagggcc tcgaaaacga cgtggcggtg gtcctttcgg gatgtttcgg attttgcgaa
     3061 aagggcccca tcgtcaaagt agctccggat aatacgttct acgtccaggt cacgccggag
     3121 gacgcgggcg agatcgtggc ggagcacatc gtcaaggggc gcaaagtgga gcgcctcctg
     3181 tacgtggacc ccgaaacgaa taaccgggtc tccgactcca agcatatgga cttttacaaa
     3241 aaacagatgc gcatcgccct ccgcaactgc ggctttgtcg acccggaaaa catcggcgag
     3301 tacatcgccc gggacggcta tcaggcctta gggaccgtcc tttccaccat gacccccgcc
     3361 caggccgtcg acgtgatgaa aaagtctggc cttcgggggc gcgggggcgg cggcttcccc
     3421 accggcctca agtgggagtt tgccagcaag ttctccgccg accagaagta cgtcatttgc
     3481 aacgccgacg aaggggaccc gggggccttc atggaccgct ccatcctgga gggagatccc
     3541 cactccgtca tcgaagccat gatcatctgc ggatacgcca ttaacgccac caaaggcatg
     3601 gtctacatcc gcgccgaata ccccctggcc gtgcagcgcc tccacaaggc catcgacgac
     3661 gcccgagaga tgggactcct gggagaccac atcctggggt cggatttcag cttcgacatc
     3721 gaactgcgct atggggcggg ggccttcgtc tgtggcgaag aaacaggact cattcgctcg
     3781 ctggaaggag aacggggcga gcccaccagc aagcccccct tccccgccga atcgggcttc
     3841 tggggcaagc cgtccaacgt gaacaacgtg gagacctttg cttgtgtccc cgtgatctac
     3901 cagaaggggg ccgagtggtt ctccaagatc ggcacggaga aatcgaaggg gaccaaggtc
     3961 tttgccctgg ccggaaaggt gaacaacgtg gggttggtcg aggttcccat gggcaccacc
     4021 ctccgggaag tgatcttcga catcggcggc ggcatccggg acggcaaggc gttcaaggcc
     4081 gttcagaccg gaggtccttc gggagggtgt cttaccacca agcacctgga cactcccatc
     4141 gactttgaca acctagtggc ggcggggtcc atgatgggct cggggggcat gatcgtcatc
     4201 gacgaggata actgcatggt ctccctggcc cgattctacc tggagttcac cgtagaagag
     4261 tcctgcggca agtgcacccc ctgccgggtg ggtaacaaga ggctccatga aatactcacg
     4321 aaaatttgtc aaggccgggg cacagaagag gacctcgccc tcttgaggga cctggggaag
     4381 atgatcaaag acacctccct ctgcggcctg ggtcagacgg ctcctaaccc cgtgctctcc
     4441 acgctggaac acttctggga cgagtacgtg gcccacgtgc gggataagcg ttgccccgcc
     4501 gggatgtgca aggatctagt tcagatcgtc atcgaccccg aaaagtgcaa ggggtgcacc
     4561 atgtgcgcca aggtctgccc cgcaggagcc atctcgggga aggcacgcga aattcatgtc
     4621 atcgaccagg aaaagtgcat caagtgcggc gcctgcatcg gcgtctgcaa gttcgatgcc
     4681 atttccaagc agtgagtgcg ggggtgcgat ccagatgatt agcctgaata tcgacggaaa
     4741 gtacgtaaag gtccccgaag gcagcaccat cctcaccgct gcccgggccc tcggcatcga
     4801 cattcccacc ctgtgtcacc tccacctcca cggcacgggc atggtgaaca aggccgcctc
     4861 ctgtcgcatc tgtgtggtgg aagtcaaggg gaggcgtaac ttggcccccg cctgcgccac
     4921 gccggtggtc gagggcatgg aggtgcgcac caacaccatc cgggtcatga acgcccggaa
     4981 gatggtcatg gagctcctgc tctccgatca tcccaaagag tgtctcacct gcgccaagag
     5041 cggttcctgc gagctacagg agctggcgga gcgcttctgt ctgcgggaaa gccgcttcga
     5101 aggaggcgat cagtccacct acgcccacga ccgttccgcc gccatcgagc gggatatgga
     5161 caaatgcgtc atgtgccgcc gctgcgagac catgtgcaac gaagtccaga ccgtgggggc
     5221 cctctccggg atcaaccggg gatttaaatc cgtggtagcc cccgctttcg aaatgcccct
     5281 ggaggaatcg gtgtgcactt tctgcggcca gtgcaccgcc gtgtgcccca cgggggccct
     5341 ggtggaaagg gattactcct ggaaggtttt tgaagctctg gcggaccccg acaaggtcgt
     5401 cgtcgtccag accgctcctg cggtgcgggc cgccctgggc gaggcctttg gcatgcctcc
     5461 ggggaccctg gtcaccggca agatggttgc cgccctgcgg gccatgggat ttgaccacgt
     5521 tttcgacacg gacttcgccg ccgacctcac catcatggaa gagggaagcg agctactgga
     5581 ccgcctcacc cgcttcctgg agggggacac ctccgtacgt cttcccatcc tcacctcctg
     5641 ctgccccgcc tgggtgaagt tcttcgaaca caagttccag gacatgctgg acgttccctc
     5701 caccgccaag tccccccagc agatgtttgg cgccatagcc aagacctatt ttgcgggtaa
     5761 actgggcatt ccccgggaaa agatggtggt ggtctcggtg atgccttgcc tggcgaagaa
     5821 gtacgagtgc tcccgccccg agttcttcgt gaacggcaat cccgatgtgg atctttccat
     5881 ctctacccgg gagctcgccc acatgatcaa acgggccaat attgacttcg taaacttgcc
     5941 cgacgaggac ttcgatggcc ccatgggaga atccacaggg gcgggggtca tcttcggcac
     6001 cacggggggc gtcatcgaag ccgccacccg tacggcggtg gagatctaca cgaaaaaacc
     6061 cctggattcg gtggatttcc tcgcccttcg cggctttgac ggatgccggg aggcccgcgt
     6121 caaggtggga gacctagaac tgcgcattgg catcgcccac ggcctgggca acgcccgcca
     6181 cctgctggaa agcatccgca agggcgaggt ggagcccttc cacgccatcg agatcatggc
     6241 ctgccccggg ggttgcgtgg gaggcggcgg ccagccctac caccacggga acatgaacat
     6301 cgtgcggaag cgcgccgagg ccatctatcg ggaggacgcc gccaagcccc tgcgtaagtc
     6361 ccacgagaac cccgagatcg tccggctcta cgaggagttc ttgggtaagc ccctggggga
     6421 aaagtcccac cacctgttgc atacccacta cttcaagcgg cataaggtct aaagggacca
     6481 cgaccgttat aggcgagtaa acaagggggc tcgggcgatg gatccaccgc ccgagcccct
     6541 ctctttcttt tgccgtgtcc ccacgtccca gccccgaagg tccagccctt tacgaagaag
     6601 tcccctagga gattagggcg attcctgccc tcccccagct tttatccctt tagggtaggc
     6661 ttcggtgatc tctccggagg gagatcgcac cagtactacc acctggcggt cataacgccg
     6721 cagaaaggct tcggcatcca tcttttggcc ctcgtggtcc agaaaaacgg tgaggttcct
     6781 atcccaggaa aatctttcat gtttcatgtc cctcatgccc acgatctccg gggggacgag
     6841 gataaggtcc accagaagca cttccacttc agagggagag accaccccct cccccggcag
     6901 aggagtcatc atggtgcccc acaagaccat tatcaacaaa attccattcc acacgccttt
     6961 ttttgtcacg gtcaatccct ccagtacaac acttcctgtg agctgtctga atcatccccg
     7021 gctcccatgc cggaagggat gtccatcacc gctatacctt catttttcgt gagccctttt
     7081 atgtcgccat cccccatgtt tgtccccgtg gcggagaaga tgcccgtgag gtagagcttg
     7141 ctcccggaga agctcacact gctgatcttc atgccggaga tctccaccac ttttttgccg
     7201 ttggagttcc aggcccctcg accgcttttg gcgtttacga tgtatagccg gcctatcccc
     7261 cccgtgccgc accggtccag ggttggacaa aaggtggcca gggccacgaa gcttccggcg
     7321 gagcggtgag taaaggccgg agcggcggag agatactctc ccggcgtcag gtcgaagtac
     7381 cacccatcgg ggtcggaaga ggtactcccc acgtccttgg aagagagctt ttgaaggtgg
     7441 gagaggtcct ttttgagggg ggcagcttcg aatccccctg cgttgacgcc aaaaatgtag
     7501 tttttggcgt tgtagtccct gttccaaacc ccctcgggat tacccgtgcc cccgtaaagc
     7561 cagatctctc cgtttatgat ccccgccccc agcccttggt ttatagccac ggatttattg
     7621 ggatccaggc ggaaaatttg atccacggag acgagccctt tgccccaagc cttgaaaatg
     7681 ttcccggcgg cgtcggggat gaaaactccc tcccatatgc cgtcgctacg ggaagtgtag
     7741 ggatagacgg gggaagatac catgtccaaa ggggtccccc atcggtcctc ggagaccagg
     7801 cggaggaccc gaccgctttg gatatccacc acgaagagtc cccgcccctc ggtggtgggc
     7861 actccctttt tctctcccgt gagcccccct ccgaaaagcg ccacgaaggg gtactctcct
     7921 ccgaggggta tccggccgat gaagggcaca ctggtggtca tgcccagacg gcggtaggcg
     7981 ctcaccagag agaaatcggg ggatgcggct tccaggttag cggagacatc gacgatcact
     8041 ggatccttct tcgacgcccg ggtgtaggtg cgatgggtaa aggcgtgcaa catggcgctt
     8101 gtccctgatc ctcccccatc ttctttctcc tcggccagga gggacttcca ccacagcacc
     8161 tggtgcaggg gctggggggt ggcggtggcg aggccggagt tttcgtagtt ggcattgtcc
     8221 accgcccaga ggaatcgagg agtatccggg ctggtgatgt ccagggcgta gagccccttg
     8281 cccccgaacc ccagacaccc caggagcacg gtgcggtagc tccactttcc gtccagcttg
     8341 aggcgcacgt ccttcaccgc cagggggccg tccagcagat ggcgaggcac agaagtccca
     8401 tcggtgatcc aagagggcga aagcccccct ttggggtcca aggggttcga tcccagcaac
     8461 accttcgtcc ccaagatgcg ccgccccgca aggatctggg gggggataaa ggcccagcgc
     8521 tcctctccgt ggttcagggc ccttccgtcg gcgtcccggc tgcgggcctc gaaggcgtga
     8581 agcatgcctc cgttggactg gacgtaaacc cgggcgtctc ggctgcggag ggcccaggca
     8641 aaggcggagt agctcgcatc ggggtaggtc ccccagggtt ttcgcaccac aaagaggccg
     8701 gaacggttta tgtcggcgag cttccagcgc tccggggggg atccgggctt ggcaaactcg
     8761 tttttcccca aggtccatcg gatgaggggc ttgatcttcc cctccagatc cgccgctccg
     8821 tcgacgttac accagatctc cggggccagg gacgccgcat tgccttcgga aaagcgcacc
     8881 aaattgctcc ccccggggtc ccggggaagg gaggcgctcc agttggcggt ataaacccgg
     8941 cggtcggacc aagaacgggc ctgcaacttg gctccggcct cccagattgt cttgcccgtc
     9001 aagtttcccg ccgcatccat cccgaacttg tacagatgtc cctgccactg gtcggagggc
     9061 tcggtggtga aggtgggcac gtaggccgcc ccttcgtcct gagggtccat tctggggggg
     9121 ctcaccacag gggtcgaggg ggatccgctc acctgacgca cccggaggat cacatcttgg
     9181 aaggcccgga cgagctcgga ggtatcccgg gcgaagtacg cctgggcact gtcatttagt
     9241 tttccgtcat cgccgtaatc cgccatctta ttcagggtgg ccgccagggt gggctccttg
     9301 gcgggatcca cgaagccgat gaccatggtg cgcacggggt aattcatgtc ttccagcttt
     9361 ttccccacgt agagctttcc cgtgttatcg taaaggtttt ttacggccag ggcggggtct
     9421 tcccccaacc ctttgccctt ggtgtcgtcg ttgccatcgc agcagaccac gagccagttg
     9481 tcctgacagt aggagaccac agggcttcgg gtggagtttt tgaaaaagtc ata
//
LOCUS       DAOOER010000067         9452 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_7699_length_9452_cov_7.338725, whole genome shotgun sequence.
ACCESSION   DAOOER010000067 DAOOER010000000
VERSION     DAOOER010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 9452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9452
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7699_length_9452_cov_7.338725"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..906
                     /locus_tag="PL162_07970"
     CDS             73..906
                     /locus_tag="PL162_07970"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQF91990.1"
                     /translation="MHEVLLLRVRQMLALRRSPEEALAALRQSFEAFVARFFPEGPLP
                     YPLELKRQHSARVASLALRIARELGWQGWPQAAAEGAGWLHDLGRFPQFARFQTFQDH
                     RSLDHGEEGFRILEGEAFLDGWPPEERLDVASAVRFHNKRRIPEECPEGHLPLARLVR
                     DADKIDVFEVVQDAIDEGRTGELLSGLTPDLAPSPGFLAELSPGSPSPAYGLLRSLGD
                     FLLLQLSWVYDMNYAPSFRLLRSSGVLDRMTSRLPASLAVQRFIRAGLRHVVQRAEET
                     S"
     gene            1040..1435
                     /locus_tag="PL162_07975"
     CDS             1040..1435
                     /locus_tag="PL162_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQF91991.1"
                     /translation="MGHRILVTDDTAFMRLLLQKILTEGGYDVVGEADNGKEAVRLFE
                     RHRPDLVTMDITMPILNGVEALKRIRAVDPDARVLMVSAMGQKNMVMQALREGAQGFL
                     IKPFEKEKVLQAVAEVLATPEDPPSPETP"
     gene            complement(1473..2993)
                     /locus_tag="PL162_07980"
     CDS             complement(1473..2993)
                     /locus_tag="PL162_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HQF91992.1"
                     /translation="MVKIFLEVTLSMAMAFESGASGTPLEVERVTLFPRGGEISLAIP
                     PGPFDITLPGPFQVGTVRLNPAPGEPFPPQPQAREEPLPGWIPPALRPLADRVDELRR
                     RALEEEASRDALGKVMEKLDGFLPEGEGALGQIQATEALRREYALRKGEADRRAQEAR
                     DLYDAWEEELGRRLPPGDRGVVVRGAGGEGGGRHLVVRTDQMGWDHLYRLELDSATGR
                     IVGSLRAMVWNLSGVDWDAPATLHTARPSGTAALPRVAPLVVSASEKRGGGGGDDFVG
                     PPALYAPEALPAPSSPQARGRAPEREDSLRDTAFVVDRLEVPGDGTSRDLLLESFELQ
                     GEVVLVAVPSRREEAWILVEVASADRTFLGGACDLVVDGTSAGLSFMPDQGAGQPLSL
                     VFGTAPAVKAERLSRVGSEGEAWLGGGHRDDGYRITVTNGLPDRRVVRVVDRIPTSAQ
                     EGVRVENVSISPAPVKNEEGVLTWEIPVDPGEKGTVVVSWRVTFPRDKELVFRDKR"
     gene            complement(3162..3458)
                     /locus_tag="PL162_07985"
     CDS             complement(3162..3458)
                     /locus_tag="PL162_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HQF91993.1"
                     /translation="MGEHVNSVTLVGALHFIKGTQVKEASGGRYFRFSLKLDSVDRAG
                     IPRHDYILVRAFDPEAQAWIREQEEGALIRLEGSVRASVGSGEMYVLAEKLERG"
     gene            complement(3790..4164)
                     /locus_tag="PL162_07990"
     CDS             complement(3790..4164)
                     /locus_tag="PL162_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91994.1"
                     /translation="MNWQKKAGILALCTFLAVGVGALPAFALRAVAGVPVVVAYPDYC
                     TGYVVSAGGRVPCSVVLTRDGYPALPNCGGGMVYVAGTRYYMPCYAPMVPVMVPAAAP
                     VMPVTAYYPAGGPCYPGVVVVP"
     gene            complement(4229..5098)
                     /locus_tag="PL162_07995"
     CDS             complement(4229..5098)
                     /locus_tag="PL162_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91995.1"
                     /translation="MVHISAKRWAGIALGALAWVGAMAWGVEAQTYIAEPVLVTQPAY
                     AGLSFYVYRPYNMPAGWFVTRDGNYPVIQDRGGVWVYGYTVEGGALVPSGYVVGSVDP
                     RTLASPPVPQPPLPAPAPQPRQPQRVAPPPPAPQPQPQFLPPPPPGVMVPQGCAGATV
                     PMVSAPYVPSWALDKNFLEMSHWSRLVDRVAILHRPRTPVAWKGDNPTVLFAWTGRHW
                     YAIRPARGETPAQALEHRLYDLLMMMRQNGMVWNDGETPALANQALLWGYLWLGSVAP
                     AQVGGPPVDLSER"
     gene            complement(5170..6051)
                     /locus_tag="PL162_08000"
     CDS             complement(5170..6051)
                     /locus_tag="PL162_08000"
                     /inference="COORDINATES: protein motif:HMM:NF036497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HQF91996.1"
                     /translation="MVDLPWRVFGRTFALGALMVALFAPGARGELLSGVEMGPPDGGG
                     RIPVVRVRSDSPGALAGLRPGDWVVELDGVNIRGRDPAEVSHALERTLGGGFSAVVIF
                     VRDQGSQVARLRPPRPLHHQRVFLDFRRDYLRIAAESDALWEGARRGMEGALFQGFPE
                     RSLEELLRETLWKIRALGTRMENMAIPSFVPDLRAGTLMGEARNSGYRALRKREEALR
                     ILADYEERRRTGAPFEHNLWLRLQGYDREVRGLMARGERDLLEGLSLVARHDAAFGDL
                     LAQEREEEAMRYRIAPR"
     gene            complement(6152..6943)
                     /locus_tag="PL162_08005"
     CDS             complement(6152..6943)
                     /locus_tag="PL162_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF91997.1"
                     /translation="MDPLAEDGRRRGRALWGTLLVAFLVARGALFPAEGSGPEKALDP
                     GELLPVGQPLFGTSFFYVHRPQSLPEGWFATLEGYPVRPLSGGEWVFGFLTPGGWRST
                     GCMVGTLDPAAVGILPAVPPRSLPPGRPLLPGGPCLGMGAPTLSLGEGGIPSWASDPL
                     FQEVKTWRATVDRMAVLVKVPVVLAWKGDHPEVVHAWTGQDWLSLYPRPGEDLDRLLA
                     RHLYTLVQLLHRGAFVWTPGDTALLASQCTSWGYLWSGVLALEMR"
     gene            complement(7038..7337)
                     /locus_tag="PL162_08010"
     CDS             complement(7038..7337)
                     /locus_tag="PL162_08010"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HQF91998.1"
                     /translation="MDDRRPPRSSEEELHLEIAGEVQGVGFRVGLYREAQRRDVTGWV
                     RNRSDGRVEALLQGAREDLEVLEEWCRRGPRYARVEAVEARWEPRGAKCPSFEID"
     gene            complement(7349..8728)
                     /locus_tag="PL162_08015"
     CDS             complement(7349..8728)
                     /locus_tag="PL162_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HQF91999.1"
                     /translation="MALTEYVGLRQINGPFVLVEGVAGAGFGELVDVVLSEGGGGGGG
                     VVALEEGRALVQVFSGTGDLVPESTRTRFLGKPLEVALAPSILGRTFSGLGEPRDGCG
                     PVYGGVPRDVNGQALNPMARLYPRDFIHTGLSAIDTLMTLIRGQKLPIFSGNGLPHNQ
                     LAVQIATQSRLVGAENFAVVFAGVGIKHDDAAFFTEELIDKGRTNNIVMYLNLADDPV
                     IERIATPRFALTAAEYLAFDLHMHVLVIITDMTNYCEALRELGVARGEVPSRKGYPAY
                     LYSDLASIYERAGVLRDQSGSVTQLPILSMPNDDITHPIPDLTGFITEGQIVLSRELD
                     AQGIYPPIDVLTSLSRLMKDGIGKGYTRDDHPNLASQLFASYSRVQEVRSLAGVVGED
                     ELGKGDKASLAFGEGFEKRFLRQGRDEDREITASLDLGWELLTTLPRTELTRVTLAQI
                     RAHLGGAPR"
     gene            complement(8719..>9452)
                     /locus_tag="PL162_08020"
     CDS             complement(8719..>9452)
                     /locus_tag="PL162_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HQF92000.1"
                     /translation="EGFPAYLPTRLGEFYERAGRVDTLGGEQGSVSIIGAVSPPGGDF
                     TEPVTRHTKRFIRCFWALDKGLANARHFPAISWLESYSEYAREVEEWFATHVNPRFAA
                     RRDQARALLAEDEKVQQIIRLVGEDVLPDDQRLTVFCAFLIKNGYLQQSAFGADSYYP
                     PEAAFGILDLILHFFDKAREMVKRGVPLSLLTDLEEVLAITHLRELPEEQRGSIPELK
                     GRLDARLDKVGMERMARSGGTSSWR"
BASE COUNT         1592 a         3451 c         2941 g         1468 t
ORIGIN      
        1 gggggccccc gtgggggtct ccacccggga catgtgcccg gggtgcggcg ccctcctggt
       61 gcgcgaggag gggtgcatga agtgcttctc ctgcgggtac gccaaatgct agccctccgg
      121 cgctcccccg aggaggccct ggccgccctg cgccagagct tcgaggcctt cgtcgcccgc
      181 ttctttccgg agggccccct cccttacccc ttggagctca agcgccagca ctccgcccgg
      241 gtggcctccc tggcccttcg catcgcccgg gagctgggat ggcaggggtg gccccaagcc
      301 gccgccgagg gggcggggtg gctccacgac ctggggcgct ttccccagtt cgcccgcttc
      361 cagaccttcc aggaccaccg atccctggac cacggcgagg agggcttccg gatcctggag
      421 ggagaggcct tcctcgacgg ttggcccccg gaggagcgcc tcgacgtggc gtcggcggtg
      481 aggttccaca acaagcgtcg catccccgag gaatgccccg aagggcacct ccccctggcc
      541 cggctggtcc gggacgcgga caagatcgat gtcttcgagg tggtccagga cgccatcgac
      601 gaggggcgca ccggagagct cctctccggc ctcacccccg accttgcccc ctctccgggc
      661 tttttggccg agctatcgcc gggatcaccc tctccggcct acgggctcct gcgctccctg
      721 ggggattttc tgcttcttca gctctcctgg gtatacgaca tgaactacgc cccgtccttt
      781 cgcctcctcc ggagctccgg ggtcttggac cggatgacct cccgcctccc cgcctccctt
      841 gccgtccagc gcttcatccg ggcgggactc cggcacgtgg tccagagggc cgaggagacc
      901 tcgtgaggga gaaggggacc tcgggcttag gggctccccc ttgcgccctg ctcccccttg
      961 ccgtatgctt tggaggagca aaagggggcg acaccggcgg cctccgggcc ggacgagtcc
     1021 taagggggcg aggaattaga tgggtcaccg catattggtc accgacgaca cggcgttcat
     1081 gcgactcctc ttgcagaaga tcctgacaga gggggggtac gacgtggtgg gggaggcgga
     1141 caacggcaag gaggcggtac gcctcttcga gaggcaccgc cccgacctgg tgaccatgga
     1201 tatcaccatg cccatcctca acggcgtgga ggccctgaag cgcatccggg ccgtcgaccc
     1261 cgacgcccga gtgctcatgg tgagcgccat gggtcagaag aacatggtga tgcaggccct
     1321 acgcgaaggg gcccagggct ttttgatcaa acccttcgag aaagagaagg tgctccaggc
     1381 cgtggcggag gtcctggcga ctccggagga tcccccttct ccggagacgc cctaggaccc
     1441 cggaaggggt accccctccg gggtcctagg cctcatcttt tgtcccgaaa gaccagttct
     1501 ttatcccggg gaaaggtcac ccgccaggag acgaccacgg tgcccttttc tccgggatcc
     1561 acggggatct cccaggtgag caccccttct tcgtttttca ccggggcggg ggagatggag
     1621 acgttttcca ccctcacgcc ctcctgggcg ctcgtgggaa tgcgatccac cacccgaacc
     1681 acccgccggt cgggaaggcc gttggtgacc gtgatgcgat acccgtcgtc ccgatgcccc
     1741 cctcccagcc acgcctcccc ctcgctcccc acccgggaaa ggcgctccgc cttcaccgca
     1801 ggcgccgttc cgaagaccag ggagaggggc tgtcccgccc cctgatcggg catgaaagag
     1861 agccccgccg aggttccatc caccaccaga tcacaggctc cccctaggaa ggtccggtcc
     1921 gccgaagcca cctccaccag gatccaggcc tcctcccggc gcgatggcac cgccaccagc
     1981 accacctccc cctggagctc gaagctctcc aggaggagat cccgggaggt gccgtcgccg
     2041 gggacctcca gccggtccac gacaaaggcc gtatcccgca gggagtcctc tcgctccggg
     2101 gcccgccccc gggcttgggg agacgaaggg gcgggaagcg cctccggggc gtagagggcg
     2161 gggggcccta cgaagtcgtc gccgccgccg ccgcctcttt tctccgacgc cgacaccacc
     2221 agcggcgcca cccggggcag cgcggcggtc ccggaggggc gggcggtgtg gagcgtcgcc
     2281 ggggcgtccc aatccacccc cgagaggttc cacaccatgg cccgaaggga gcccacgatg
     2341 cgccccgtgg cgctgtccag ttccagccgg tagaggtgat cccaacccat ttgatccgtc
     2401 cggaccacca gatgtctccc cccaccctct cccccggcgc cgcgaacgac cacgccccga
     2461 tcccccggag gaaggcgacg tcccagctcc tcctcccagg cgtcgtagag atcccgggct
     2521 tcctgggccc tccggtcggc ctcccccttg cgcagggcgt actccctccg gagggcctcc
     2581 gtggcctgga tctgacccag ggccccctct ccctcgggaa ggaagccgtc gagcttttcc
     2641 atgaccttac ccagggcatc ccgggaggcc tcctcctcca aggctcgacg cctcaactcg
     2701 tccacccggt ccgccagggg gcgcagcgcc gggggaatcc atcccggcag gggctcctcc
     2761 cgcgcctggg gttggggcgg aaagggttcc cccggggcgg gattcaggcg cacggtcccc
     2821 acctggaagg gccccggcag ggtgatgtca aagggccccg gggggatcgc caaggaaatc
     2881 tcgccccccc gggggaaaag ggtcacccgc tccacctcca ggggcgtccc cgacgccccc
     2941 gattcaaagg ccatggccat gctcagggtc acctccaaaa atatttttac caatttttta
     3001 acgtttcatc cctcctccgg acaaaggctc ctcccaaggc ccggggcgcg ccccctgccc
     3061 ggacgacccc tcgggtcggg caagggggcg cgccccgggc ctttcggtct gtggcggccc
     3121 ctccagaatc ggaggaggcc tccccggggg gaattatctc cctagccccg ctccagtttt
     3181 tcggccaaca cgtacatctc gccactgccg acggaggcgc ggaccgagcc ctccaggcga
     3241 atgagggccc cttcctcctg ctccctgatc caggcctggg cttcgggatc gaaggcccgc
     3301 accaggatgt agtcatgccg gggaatcccc gcccgatcca cactatccag cttcagagaa
     3361 aagcgaaaat aacgcccccc gctggcctct ttgacctggg tccccttgat gaaatgtaac
     3421 gcccccacca acgtgacgct gttgacgtgc tcccccatgc cccgtcctcc cctcaaatcg
     3481 tgctctccgg acctcgccga aagtgacaaa agactcgccc acctgccgcg ccccggggga
     3541 gcgatctccc cccctttttg agaaaagggg ccaagctcct ccgggccttc cccggggtta
     3601 atcgcccgat ggatgccccg tcgatccgcc ccctgatctc gcccccttca aggtcttttc
     3661 ttgtcgacga atgaccgcca ccgtaggacc cttcggccca aaaggataaa gcgacaaccc
     3721 agcccggagc cagatgtcgc ttcatcgcga tagagcgcac tgatctttcc tacccaaacc
     3781 tccgatctct taggggacca ccacgacccc ggggtaacag ggacccccgg cgggataata
     3841 ggcggtgacg ggcatcacag gcgccgccgc aggaaccatc acgggcacca tgggcgcata
     3901 acagggcata taatagcgcg tccccgccac gtagaccatc cctccgccgc agttcggcag
     3961 ggccggataa ccgtcccgcg tgagcaccac ggagcagggc acccttcccc ccgcagagac
     4021 cacgtagccc gtgcagtaat ccggataggc caccaccaca ggcacccccg ccacggcgcg
     4081 cagggcaaag gcgggcagag cccccacccc cacagccaag aaagtacaga gcgccaaaat
     4141 gcctgccttc ttctgccagt tcatgtcgct tcagtcctcc ttcctacttt ccgcctagaa
     4201 gcaccccttc cccccaggga cttagccctc acctctccga aaggtccacg gggggccccc
     4261 ccacctgagc cggcgccaca ctccccagcc acaggtaccc ccagagcaac gcctgattcg
     4321 ccaacgccgg agtttccccg tcgttccaca ccatgccgtt ttggcgcatc atcatcagga
     4381 ggtcgtacaa gcggtgttcc aaggcctggg ccggggtttc tccccgggcg gggcggatgg
     4441 cataccaatg cctccccgtc caggcaaaga gcaccgtggg gttatccccc ttccacgcca
     4501 cgggggtccg ggggcggtgc aggatggcca cccggtccac caggcgagac cagtgggaca
     4561 tctccaggaa gttcttgtcc agggcccagg agggcacgta aggggcggac accatcggca
     4621 ccgtcgcccc ggcacacccc tgggggacca tcaccccggg agggggtggg ggcaaaaact
     4681 ggggctgggg ctggggcgct ggaggaggag gcgccacgcg ctggggctgc cgaggttgag
     4741 gcgcgggagc cgggaggggg ggctggggca ccggagggct ggccagcgtc cgggggtcca
     4801 ccgatcccac cacgtacccc gaggggacga gggcgccccc ttccacggtg tagccgtaga
     4861 cccatacgcc gcccctatcc tggatcaccg gataattccc atcccgggtg acgaaccacc
     4921 cggcgggcat gttgtacggc cggtaaacgt aaaagctcaa ccccgcatag gcgggctgcg
     4981 tcaccaacac cggctcggcg atgtaggtct gggcctccac cccccaggcc atcgccccga
     5041 cccaggccaa ggcccccaag gcaatccccg cccaccgttt cgcgcttatg tgcaccacgt
     5101 cccatcccct cctttaaaat taatgtagag gcgccccact cctccccatc cttcgcgagg
     5161 ggggcgcctc tacctcgggg cgatgcggta ccgcatcgcc tcttcttcgc gctcttgtgc
     5221 caaaaggtcg ccgaaggcgg cgtcgtgacg ggccaccaac gagagcccct ccaggagatc
     5281 tcgctctccc cgggccatta gcccgcgcac ttcccggtcg tagccctgga gccggagcca
     5341 gaggttatgc tcgaaggggg cccccgttcg tcgtcgctcc tcgtaatccg ccaggatcct
     5401 caaggcctct tccctcttcc tcagggcccg atagccggag ttcctcgcct cgcccatcag
     5461 agtgcccgcc cgaaggtccg gcacgaagga ggggatggcc atattctcca tgcgagtgcc
     5521 cagcgcccgg atcttccaga gggtctcccg gagaagctcc tccagggatc tttccggaaa
     5581 tccctgaaac agggcccctt ccatcccccg cctcgcccct tcccagaggg cgtcgctctc
     5641 ggcggcaatc cggaggtaat ccctccgaaa gtcgaggaag acccgctggt ggtgcagggg
     5701 gcgcgggggg cgcaggcggg ccacctggga tccctgatcc cggacgaaaa tcaccacggc
     5761 ggagaatcct ccccccaggg tccgctccag ggcgtggctc acctcggcgg gatcccgccc
     5821 tcggatgttc accccgtcga gctccaccac ccagtccccc ggcctcaatc ccgccagggc
     5881 cccgggggag tcgctcctca cccgcaccac ggggatgcgc cccccgccgt cggggggacc
     5941 catctccacg ccggaaagga gctcccccct cgcccctggg gcgaaaagcg ccaccatcag
     6001 ggcgcccagg gcgaaggtcc gcccgaacac gcgccaggga agatctacca tcctcactcc
     6061 gtcgcccccc ttctagacct caggacggga taagggccct ccgccgaggc ggcctccgaa
     6121 gccgccggcg gggaagctct tttcacgatg atcaccgcat ctccagggcc aggacgccgg
     6181 accagaggta cccccacgag gtgcactggg aggccaaaag ggccgtatcc cccggggtcc
     6241 agacgaaggc cccccggtgg agcagctgga ccaaggtgta gaggtgtcgg gccaaaaggc
     6301 ggtccagatc ctcccccggc cggggataaa gggagagcca gtcctgcccc gtccaggcat
     6361 ggaccacctc ggggtggtct cccttccagg ccaacaccac gggaactttc accagcaccg
     6421 ccatgcggtc cacggtggcg cgccacgtct ttacctcctg aaacaaagga tccgaggccc
     6481 aggacgggat ccccccttcc ccgagggaaa gggtcggcgc tcccatcccg aggcacggcc
     6541 ctccggggag gagaggccgc cccggaggga ggctccgggg gggcacggcg gggaggatgc
     6601 ccaccgccgc agggtccagg gtgcccacca tgcacccggt ggaacgccac cctccggggg
     6661 tgaggaagcc gaagacccat tctccccccg aaagggggcg cacgggatag ccttcgaggg
     6721 tggcaaacca cccctcgggg aggctttggg ggcggtggac gtaaaaaaag ctcgtcccga
     6781 aaaggggttg accaacgggg aggagctcgc cggggtcaag ggccttctcc ggccccgatc
     6841 cttccgccgg gaaaagggca cctctcgcca caagaaacgc caccaggagc gtcccccaaa
     6901 gggcacgccc tcgccttctt ccgtcctccg ccagcggatc catggccgct cctcccttcg
     6961 gttagacctg gggatggatc gttgtttctc tcttcactat aacaagatcg tcgccttttg
     7021 aaaagggccc cggggggtca gtcgatctcg aaggaaggac acttcgcccc ccggggctcc
     7081 catcgggctt ccaccgcctc cacccgggcg taccgaggcc cccggcgaca ccactcctcc
     7141 aggacttcca agtcctcccg ggccccctgg agcagggcct ccacccgacc gtcggagcgg
     7201 tttctcaccc accccgtcac gtcccggcgc tgggcctccc ggtagagccc cacgcgaaag
     7261 cccaccccct ggacctctcc cgctatttcc agatgaagct cctcttccga ggagcgcgga
     7321 ggacgccggt cgtccacgac ggcccctcct accggggagc gccccccagg tgggcccgga
     7381 tttgggccag ggtcacccgg gtcagctccg tgcggggcag ggtggtcaaa agctcccatc
     7441 ccagatccag tgaggcggtg atctcccggt cttcgtccct cccctgtcgg aggaagcgct
     7501 tctcgaagcc ttcgccgaag gccagggagg ccttgtcccc cttgcccagt tcgtcctccc
     7561 ccaccacccc tgcaagggag cgcacctctt gtacccggct gtaggaggca aagagctggg
     7621 aggccaggtt ggggtggtcg tcccgggtgt atcccttgcc gatgccgtct ttcatgagac
     7681 gggagaggct ggtgagcacg tcgatggggg ggtagatccc ctgggcgtcc agctcccggg
     7741 agaggacgat ctgcccctcg gtgatgaatc ccgtgaggtc ggggatgggg tgggtgatgt
     7801 cgtcgttggg catggagagg atcgggagct gggtgacact ccccgactga tcccggagca
     7861 cccccgcccg ctcgtagatg gacgccaggt cgctgtacag gtacgcgggg taccccttgc
     7921 ggctcggcac ctctccccgg gccaccccca gctctcgcag agcctcgcag tagttggtca
     7981 tgtcggtgat gatcaccagc acgtgcatgt ggaggtcgaa ggcgaggtac tccgccgccg
     8041 tgagggcgaa cctcggcgtg gcgatacgct cgatgacggg gtcatccgcc aggttgaggt
     8101 acatgacaat gttgttggtc cgccccttgt cgatgagctc ttcggtgaag aaggccgcat
     8161 cgtcgtgctt gatccccacc ccggcgaaga ccacggcgaa gttctccgcc cccaccaggc
     8221 gggactgggt ggcgatctgc accgccaact ggttgtgggg caggccgttg cccgagaaga
     8281 tggggagctt ttgcccccgg atcagggtca tgagggtgtc gatggccgaa aggcccgtat
     8341 ggatgaaatc tcgggggtaa agccgggcca tggggttcag ggcctggccg ttcacgtccc
     8401 ggggcacgcc cccatagacg gggccgcatc catcccgggg ctccccgagg cccgagaagg
     8461 tgcgccccag aatggaaggg gccaaagcca cctccagagg ttttcccaga aatcgggtcc
     8521 gggtgctttc gggcaccaga tcccccgtgc cggaaaagac ctgcaccagg gctctcccct
     8581 cttccagggc caccaccccc cccccccccc cccccccttc ggagagcacc acgtccacca
     8641 gctctccgaa acccgccccc gccacccctt ccaccagcac gaaggggccg ttgatctgcc
     8701 gcagtcccac atattccgtc agcgccatga ggaggtccct ccgctcctgg ccatgcgctc
     8761 catgcccacc ttgtccaggc gggcgtcgag gcggcccttc agttcgggaa tggatccccg
     8821 ctgttcctcg gggagttccc gcaggtgggt gatggccaac acctcttcca gatccgtgag
     8881 cagcgaaagg ggcacccccc gcttcaccat ctcccgggcc ttgtcgaaga agtggaggat
     8941 cagatccaga atgccgaaag ccgcctcggg gggatagtag gaatccgcgc cgaaggcgct
     9001 ctgctggaga tagccgtttt tgatgagaaa ggcgcaaaaa accgtgaggc gctggtcgtc
     9061 ggggagcacg tcctctccca ccagacggat gatctgctgc accttctcgt cctccgccag
     9121 cagggcccgg gcctgatccc gccgcgccgc gaaacgggga ttcacgtggg tggcaaacca
     9181 ctcctccacc tcccgggcgt actcgctgta ggactccagc cagctgatgg cgggaaagtg
     9241 ccgggcgttg gccaatccct tgtccagggc ccagaagcac cggatgaagc gcttggtgtg
     9301 ccgggtgacg ggctcggtga agtccccccc cggaggggag accgcaccga tgatggacac
     9361 gctcccctgc tctcctccca gggtgtccac ccggcccgcg cgctcgtaga actccccgag
     9421 gcgggtgggc agataggcgg ggaagccctc ct
//
LOCUS       DAOOER010000068         8967 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_8179_length_8967_cov_9.050382, whole genome shotgun sequence.
ACCESSION   DAOOER010000068 DAOOER010000000
VERSION     DAOOER010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 8967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8967
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8179_length_8967_cov_9.050382"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            174..818
                     /gene="infC"
                     /locus_tag="PL162_08025"
     CDS             174..818
                     /gene="infC"
                     /locus_tag="PL162_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HQF92001.1"
                     /translation="MAVTKNEDEPRVNEQIRVPEVLLIDDQGVKLGVVPTRQALFLAE
                     ERELDLVEVAPLATPPVCRIMDFGKFRFQQQKKDKDARKKQKTQTIKEMKMRPKIDVH
                     DYNFKTKAIKDFLEDGHRVKVSVFFRGREMAFLDQGRVVLDRVAKDCEGLATVEMTPR
                     MEGRFMRMMLAPVPQPKKASAPAAPKEPKELKEPKKDKAPSAEAPGRGVVEDRA"
     gene            876..1073
                     /gene="rpmI"
                     /locus_tag="PL162_08030"
     CDS             876..1073
                     /gene="rpmI"
                     /locus_tag="PL162_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HQF92002.1"
                     /translation="MPKIKTHSGTKKRFRLTGTGKVAYQKNGRRHQLNCKSPKRRRTL
                     RATGILPEAQAKNIREMLPYA"
     gene            1173..1532
                     /gene="rplT"
                     /locus_tag="PL162_08035"
     CDS             1173..1532
                     /gene="rplT"
                     /locus_tag="PL162_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HQF92003.1"
                     /translation="MPRVKGGSASNCKYKRLFSITKGYFGRKKNIFKLAKQAFFRSMT
                     NAYRDRKARKRDFRRLWIIRINAAARSNGLTYSLLMNGLKRSGIEINRKMLADVAIND
                     MPAFAKLVDQAKVALGK"
     gene            1871..2857
                     /gene="pheS"
                     /locus_tag="PL162_08040"
     CDS             1871..2857
                     /gene="pheS"
                     /locus_tag="PL162_08040"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HQF92004.1"
                     /translation="MSGSSDTEALDTQRVKLLGKKGKVTALLRLLGSLPSEERPAFGA
                     AVNALREELEQLLERRFLALQKAQFARQEEKDRVDVTLPSRGRGWGSFHPVVQVMHDV
                     IDIFSGLGFSVATGPEVEDDFHNFEALNIPPSHPARDMQDTFYMEEGKLLRTHTSPVQ
                     VRSMLRWGAPVRIICPGRVYRRDSDPTHSPMFHQLEGLLVDRDVSIADLKGCLEAFAS
                     SVFNRPLDVRYRASYFPFTEPSIEMDVSCVVCEGKDPGCRVCKGTGWLEICGLGMVHP
                     NVLRAGGIDPAIYNGFAWGMGLDRIAMLRYGLRDLRLLFEGDVQFLTQGGLA"
     gene            2857..5238
                     /gene="pheT"
                     /locus_tag="PL162_08045"
     CDS             2857..5238
                     /gene="pheT"
                     /locus_tag="PL162_08045"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HQF92005.1"
                     /translation="MLLSLNWLRDLVRFSERPEVLADRLTMTGSEVESIERPGKNLSG
                     VVIARVTDLSPHPSREGLRGARVTWKGKEAFCVTGAPNVAVGDLVPYAPPGATLADGT
                     ALGVRDFDDWRSEGMMLSAEELGQPSVATDFGILILPEDAPEGEDAAVWLGLDDVILD
                     VSVTPNRGDLLSLVGLAREVHALLPSSELLPGSDFPLEGGEWPVSFDGIALQDRGCPF
                     YALGYAENLRMGPSPVRSRVRLALSGMRPISNVVDATNYVMIFWGQPLHAFDYDRLPA
                     RHILVRGASEGEVLRTLDGKDRVLVDRDLCITSGGIPVALAGVMGGEATEIVPGTQRV
                     ALESAIFAPDRVGSTSRRLGMRSEASNRFARGVDRCKSLPALRHALTLFRDWAGGDVA
                     FLPRTSGDDRPEPRTVTLRAPVLKKLTLKDDMDHAKAVLDRLGFEELSRSVEAAEFAV
                     PSWRLDVGIEEDLVEEVARLDGYDQVPSRIPSNLHHPGLPTPEVRTDRRLREIAMGRG
                     FTEVVTYSFVSPGSLERLAFPPDDPRSRPLRVANPLSAEQSVLRPSMMPGLLGVVEHN
                     LRGGWRNAIRIFEIGRVFLRQGEGHVEPSRLGGVAYAGKDRRFGEEAETFLGVKADIE
                     ALCRGCGARVEFHQGSQPFGHAGRTALITVGNVSLGYLAQIKPSLARELNVEEDLFVF
                     ELDTAPLMDWGKALFPEPARYPAAYRDVSFLASEGVPVIETAREVRRLGGERVASVRL
                     FDVYSGQGVPEGQRSLAFSVAWRHPDRTLGEGEVDSLHNALREALAARGYVLR"
     gene            5354..5656
                     /locus_tag="PL162_08050"
     CDS             5354..5656
                     /locus_tag="PL162_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92006.1"
                     /translation="MLQQIEQLEKLLDKVMERLRALEKEREEYGVSLEEQKKLVQEKE
                     LELIRLRKEIQRSVEQREREDMQARKDRAQAEQKLNELLERFRSFGGHPGGPQAHS"
     gene            5689..5910
                     /locus_tag="PL162_08055"
     CDS             5689..5910
                     /locus_tag="PL162_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92007.1"
                     /translation="MTLRLGEVTYPLRTRLDDKAWDRVRTLVGAAFDEAPRHLPREQR
                     LVLTCLQMALSLDRIGERLEAFAREENLL"
     gene            5907..6467
                     /locus_tag="PL162_08060"
     CDS             5907..6467
                     /locus_tag="PL162_08060"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HQF92008.1"
                     /translation="MNSSLVDLLLAVVTGGLVIRGLWRGLAGEVLSLLGMAGGTFLAW
                     RLGSQGGELVAKFVDLSPGTASVVAMVGIFFLVMLLAAMAERLAKALLSFTHLSLLDR
                     GAGGLCGLAKALLLVLLIYGAGLVVSGGAEPAWMGESRFMRLAGGIWPLVEGALRERG
                     VELPKNLALPSLEVGPLLDGSGTDSP"
     gene            6464..8875
                     /locus_tag="PL162_08065"
     CDS             6464..8875
                     /locus_tag="PL162_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HQF92009.1"
                     /translation="MIVAERTRRILEIDAVLGLIARFCRTPLGVRHFDVLKPAGTMEQ
                     LHQRQEVLRAYGRCRDVHGELPWDSRLQPVSPLLEEARETALLTGEELLRFRVLFLVA
                     MQLRAAVAEYRATYPALGALTQGVRDFADELALLECLSDDGRLYDSASPDLAEVRRKL
                     DVVRRDLRQRGARLLNDPGTVAKLQERVLSLRDGRFVVLVRQEHVNSFPGIAIDRSGS
                     GNSVYMEPNALVPHNNKLSLLSRDEERIERKIYRRLTEALLSRSGAIEDAEESLGLLD
                     ALYGTADFARSHRWHLPDLVPKPQFRLNRAVHPLLGPKAVPLSLSCGERFSQLVVTGP
                     NTGGKTVALKTAGVALVLAWMGLPIPASEGSVVGDIDQVAADVGDEQSIAQNLSTFSA
                     HLANIVEMLASATPRSLLLLDELGAGTDPQEGAALGIALLQTLLARRTLVLATTHHNP
                     IKRFALTTAGVETASVEFDPATLSPTYRLLMGVPGRSNALLIAQRLGMPPEVLHAARQ
                     SLAGEDVALEDLIGELQEKQGTLERAFEELAREKTAVAELRERFERKDAELDAQKETI
                     LERADRSAAKVLSEAQEEARELLKGLDPELLAAAQKALTERQEAVRRSKEAIAVRAER
                     RTERHSAPARGETVQVGSVVRLLESKVRGTVESLEGGKALVMAGGMRIEVPLKRLQLA
                     TEAPEEKNVFEDRTPGRRKTDPVHSPLSRGGISVSAASVAVPSSIMVRGMTVDEALPV
                     VERYLDQAFRHGYPSVVVIHGQGEGILRREVQALCKRLPYVLEFCLGGPGEGGLGVTV
                     VTFRK"
BASE COUNT         1576 a         2867 c         2963 g         1561 t
ORIGIN      
        1 gcggatcaag tagaggcccg acctctcgcc agctccctcc gggggcctgg cccgcgccat
       61 aagagtgttt tggtgcgctg tgtctttggg ccgggttttc ccggcccttt tttttagaaa
      121 tcctccgcag gctcaaaggc ggcggggaac ggatatgaaa ggggtggcga gctatagctg
      181 tcacgaagaa cgaggacgaa cctcgcgtca atgaacagat tcgcgttccg gaagtccttc
      241 tgatcgacga ccagggggta aagctggggg tggtgcccac ccgccaggcc ttgtttttgg
      301 cggaagaacg ggagctcgac ctggtggagg tggcccctct ggcgactccc ccggtgtgcc
      361 ggattatgga cttcggcaag ttccggttcc agcagcagaa gaaggacaaa gatgcccgga
      421 agaaacaaaa gacccagacc atcaaagaga tgaagatgcg tcccaagatc gacgtccatg
      481 actataactt caagacgaag gccatcaaag acttcctcga agatgggcat cgagtcaagg
      541 tctcggtgtt tttccggggg cgcgagatgg cgttcctcga ccagggccgg gtggtgttgg
      601 accgggtggc gaaggactgc gaggggttgg ccaccgtcga gatgacgccg cgcatggagg
      661 ggcgcttcat gaggatgatg ctggcccccg tgccccagcc caagaaggcc tctgccccgg
      721 cggcccccaa ggagccaaaa gaactaaaag aaccgaagaa ggacaaggcc ccctcggcag
      781 aagcccccgg taggggcgtc gtcgaggaca gggcttaagc cgaaagagga taaagccggg
      841 cccgtcccgg cgatttttcg aggaggttcg atgtcatgcc aaagatcaag acccattcgg
      901 ggacgaaaaa gcgctttcgt ctcacgggga cgggaaaagt ggcgtaccag aagaacggtc
      961 gtcggcacca gctgaactgc aagagcccca aacggcggcg cacgttgcgg gccacgggaa
     1021 tccttcccga agcccaggcg aaaaatatcc gggagatgct tccctacgct taaagctttt
     1081 ccgtcgtgca ggccgggggt ggagagatac aaaagcgttt cgcggggatc ttcctccagc
     1141 ctgtgacgta tttttgaaag aggtgaatgc tcatgccccg ggttaagggc ggtagcgcca
     1201 gtaattgcaa atacaagagg ctttttagta taaccaaggg ttattttggt cgcaagaaga
     1261 atatcttcaa gttggccaag caggccttct tccggtccat gaccaatgcg taccgggatc
     1321 gcaaggctcg caagcgggac ttccggcggc tttggatcat ccggatcaac gcagcggcgc
     1381 ggtccaacgg cctgacgtac agcttgctca tgaacggcct gaagcggtcg ggcattgaga
     1441 tcaatcgcaa gatgctggcg gatgtggcca tcaacgacat gcctgctttt gccaagctgg
     1501 tggaccaggc gaaggtcgcg ttggggaagt aagccctccg gacccatacg catacggcgc
     1561 tccctggggg gcgccccgtc acgttggggt tcctgcttgg tcatcgcggc aggaaccctc
     1621 ttgctttcgg ggaggccccg gagcccttcg ctctttgccc cgatccctct tcggtgactt
     1681 ccgaacgtgg gcgagggggg ggccaaggtc cgagggggac gatcccttcg atgcgagccg
     1741 tccggaggtc cctttttcag cacagggggc cccggagatt tttctaattc ccggatggga
     1801 gagggggtag gacagggtga gcgcgcggat acaagatgtt gaagcattgc gagagcaggg
     1861 ccgccaggaa atttcgggca gctcggacac ggaggctctc gatacccaac gggtgaagct
     1921 cctcggtaag aaggggaaag tcaccgcctt gctgcggctg ttgggttccc ttccttcgga
     1981 ggagcgtccc gcctttggag cggcggtgaa cgcgctgcga gaggagctgg agcagctcct
     2041 ggagaggcga ttcctggcgc tccagaaggc gcagttcgcc cgccaagagg agaaggatcg
     2101 ggtggacgtg accctgccct cccgggggcg aggctggggg agctttcatc ccgtggtgca
     2161 ggtcatgcac gacgtcatcg atatcttctc ggggctcggt ttttccgtgg ccacggggcc
     2221 cgaggtggaa gatgacttcc acaacttcga ggccctgaac attcccccgt cccatcccgc
     2281 ccgggacatg caggacacgt tctacatgga agagggcaag cttctgcgca cccatacctc
     2341 gccggtgcag gtccgatcca tgttgcgctg gggagccccc gtgcggatca tctgcccggg
     2401 gagggtctat cgccgggata gcgaccccac ccattctccc atgttccacc agctcgaagg
     2461 gttgctggtg gaccgggatg tatccatcgc cgatctcaag ggctgcctag aggcctttgc
     2521 ctcctccgtc ttcaaccgtc ccctggacgt gcgctaccgg gcgagctact tccccttcac
     2581 cgagccctcc atcgaaatgg acgtgagctg cgtcgtctgc gagggaaaag accccggatg
     2641 ccgggtgtgt aagggcacgg ggtggctcga gatctgcggt ctgggcatgg tgcaccccaa
     2701 cgtgcttcgg gcggggggca tcgaccccgc catctacaac ggtttcgcct ggggcatggg
     2761 gctcgatcgc atcgccatgc tccgctatgg tttgcgggac cttcgcctgc tcttcgaagg
     2821 cgacgtccaa ttcctgaccc aaggggggct ggcctgatgc tcctttccct gaattggctt
     2881 cgcgatctgg tgcgcttttc cgaacgcccc gaggtgctgg cggaccgcct caccatgacg
     2941 gggagcgagg tggaatccat cgaacgcccg gggaaaaatc tctccggcgt ggtcatcgcc
     3001 cgggtgaccg atctgagccc ccacccctcc cgagaggggc tccggggggc ccgggtgacc
     3061 tggaagggca aagaagcctt ctgcgtcacc ggcgccccca acgtcgccgt gggagacctg
     3121 gtcccctatg ccccccccgg cgccaccctg gcggatggca ccgccctagg agtgcgggac
     3181 ttcgacgact ggcgctccga ggggatgatg ctctcggcgg aagaattggg ccaaccctcg
     3241 gtggccaccg attttggtat cctgatcctc cccgaggacg cccccgaggg cgaagacgcc
     3301 gcagtatggc tggggcttga cgacgtgatc ctggacgtct ccgtgacgcc caaccggggg
     3361 gacctcttga gcctggtggg gctcgcccga gaggtccacg ccctgcttcc ctcctcggag
     3421 ctccttcccg ggtcagattt tcccctggaa gggggagagt ggcccgtgtc cttcgacggc
     3481 atcgccctcc aggaccgggg atgccctttc tacgccctgg gatacgcaga aaacctccgc
     3541 atgggtccct cccccgtgcg gtcccgggtg cggctggccc tttccggcat gcgccccatc
     3601 agtaacgtgg tggacgccac caactacgtc atgatcttct ggggacaacc cctccatgcc
     3661 ttcgactacg accgtcttcc cgcccgccac atcctcgtgc ggggcgccag cgagggcgaa
     3721 gtgctccgca ccctggacgg taaagatcgg gtattggtag accgggacct ttgcatcacc
     3781 agcggcggca ttcccgtcgc cctggcgggg gtcatggggg gagaggccac ggagatcgtc
     3841 ccggggaccc agcgggtggc cctggaatcg gccatcttcg cccccgatcg cgtgggatcc
     3901 acctcccggc gcctggggat gcgctccgag gcatcgaacc gctttgcccg gggagtggat
     3961 cgatgcaagt ccctccccgc cctgaggcac gccctcaccc tcttccgaga ctgggcggga
     4021 ggggatgtgg cctttctgcc ccgcacctcg ggagacgatc gccccgagcc ccggaccgtg
     4081 acccttcgcg ccccggtgct aaaaaaactt acgcttaaag acgatatgga tcacgccaag
     4141 gccgtgctgg accgcctggg cttcgaagag ctctcccgga gcgtcgaagc ggcggaattt
     4201 gccgtgccct cctggcgcct tgacgtgggc attgaagaag acctcgtcga agaagtggcc
     4261 cgcctcgacg gctacgacca ggtgccttcg cgcatcccct ccaacctgca ccacccgggg
     4321 ctgcccaccc ccgaggtccg caccgaccgt cgcctgcggg agatcgccat ggggcgaggc
     4381 ttcacggagg tggtcaccta cagcttcgtt tcccccggga gcctggagcg cctggccttt
     4441 cctccggacg acccccggtc ccgccccttg cgggtggcca atcccctgag cgcggagcag
     4501 agcgtccttc gtccctccat gatgccgggg cttttggggg tggtggagca caacctgcgg
     4561 ggggggtggc gcaacgccat tcggatcttt gaaatcggcc gggtcttcct gcgccaaggc
     4621 gaagggcacg tggaaccctc tcggctgggg ggcgtggcct atgcgggcaa ggatcgccgc
     4681 ttcggcgaag aggccgaaac ctttcttggc gtcaaggccg acatcgaagc cctctgccgg
     4741 gggtgtggcg cccgggtgga gttccatcag gggagccaac cctttggcca tgcggggcgc
     4801 accgccctga tcaccgtggg aaacgtctcc ctgggatacc tggcccagat caaaccctcc
     4861 ctggcgcggg agctcaacgt ggaagaagac ctcttcgtct ttgaactgga cacggcgccc
     4921 ctcatggact ggggcaaggc ccttttcccc gagcccgccc ggtacccggc ggcctaccgg
     4981 gacgtgtcct tcctggcctc cgaaggcgtt cccgtcatcg aaaccgcccg ggaagtccgg
     5041 cgcctggggg gcgaacgggt ggcctccgtg cggctttttg acgtctactc ggggcagggg
     5101 gttcccgagg ggcaacgcag cctggccttt tccgtggcct ggcgtcaccc cgaccgcacc
     5161 ttgggggaag gggaggtgga ctccctgcac aacgccctga gggaggccct ggcggcccgg
     5221 gggtatgtgt tgcggtagct atcccgacgc ggggcggtgt gttaaaatgt ttccccagga
     5281 ggagggcttc ggggtcagcc cggggtcttg cctcccatcc cacgtccatc caagaaaggg
     5341 gtcgttgtag ggcatgctac agcagatcga acagttggaa aaactcttgg ataaggtcat
     5401 ggagcgcctt cgagcgttgg aaaaggagcg cgaagagtac ggggtgtccc tggaggagca
     5461 aaaaaagctc gttcaggaaa aggagctgga gctgattcgc ctgcgcaaag agattcagcg
     5521 ttccgtcgaa cagagggaac gggaagacat gcaggcccgg aaggaccgtg cccaggcgga
     5581 gcaaaagctt aacgaactgc tggagcgctt ccgctccttc ggcggtcatc ccggagggcc
     5641 ccaggcccac tcatgagacc attggcggag gaggatgggc ttcgtaccgt gactttgcgc
     5701 ctgggcgaag tgacctaccc tctgaggacc cggctggacg acaaggcctg ggatagggtg
     5761 aggaccttgg tgggggccgc ctttgacgag gccccccgcc accttcctcg ggagcagcgc
     5821 ctggtcttga cctgcttgca gatggccctc tcgctggatc gcatcggaga acggctggag
     5881 gccttcgcca gagaggaaaa tctgctatga actcttccct ggtggacctg ctgctcgcgg
     5941 tggtcacggg ggggctcgtg attcggggtc tttggcgagg actggcgggg gaggtcttat
     6001 ccctgctggg catggcgggg ggaacctttt tggcctggcg cctgggctcc cagggaggag
     6061 agctggtggc gaaatttgtc gacctctccc ccggcaccgc ctccgtcgtc gccatggtgg
     6121 gaatcttttt cctcgtcatg ctcctggcgg ccatggcgga acgcctcgcc aaggccctgt
     6181 tgagctttac ccacctatcc ctcctggatc ggggcgccgg agggctctgt ggcttggcca
     6241 aggccctgtt gctggtgttg ctgatctacg gggcgggact tgtcgtatcg gggggcgcgg
     6301 aacccgcctg gatgggagaa agccgcttca tgcgcctggc gggggggata tggcccctcg
     6361 tggagggcgc cctgcgggag cgcggcgtgg aactccccaa aaatcttgcc ctgccctctc
     6421 tggaggtggg gcccttgttg gatgggagcg gaacggattc gccatgatcg ttgcggaaag
     6481 aactcgacgt attcttgaaa tcgacgcggt actgggcctg atagcccgtt tctgccgcac
     6541 acccctcggt gtgcggcatt tcgatgtatt aaagcccgcc ggcacgatgg aacagctcca
     6601 tcagcgacag gaagtgctgc gcgcctatgg gcgctgtcgg gatgtgcacg gcgagctccc
     6661 ctgggacagc cgtctccaac ccgtctcccc ccttttggag gaggcccggg aaacggccct
     6721 gctcaccgga gaagaactcc tgcgattccg ggtgctcttt cttgtggcca tgcagctccg
     6781 ggcggcggtg gcggaatatc gggccaccta tcccgccctg ggggccctca cccagggggt
     6841 gcgggacttc gccgacgaac tggcgctctt ggagtgcctc agcgacgacg gccgcctcta
     6901 cgacagcgct tcccccgacc tcgccgaggt gcggcgcaag ctcgacgtcg tccgccggga
     6961 ccttcgccag aggggggcgc gccttctcaa cgaccccggc acggtggcca aactccagga
     7021 gcgggtgctc tccctccggg acgggcgctt cgtcgtgctc gtgcgccaag agcatgtgaa
     7081 ctcctttccc ggcatcgcca tagatcggtc gggatcgggg aactcggtgt acatggagcc
     7141 gaacgccctg gtaccccata acaacaagct ctccctcctc agccgagacg aagagcgcat
     7201 cgaacgaaag atctaccgac gcctcacgga ggcccttctt tcccgcagcg gcgccatcga
     7261 agacgccgag gaatccctgg ggcttctgga cgccctgtac ggcaccgccg acttcgcccg
     7321 atcgcaccgg tggcacctcc cggacctcgt ccccaagccc cagtttcgcc tcaaccgggc
     7381 ggtgcacccc ctcctgggac ccaaggcggt gcccctctcc ctctcctgcg gcgagagatt
     7441 ttcccagctg gtggtgacgg ggcccaacac ggggggtaag accgtggccc tcaagaccgc
     7501 cggagtggcc ctggtgctgg cctggatggg ccttcccatc cccgccagcg agggctccgt
     7561 ggtgggcgac atcgaccagg tggccgccga cgtgggggat gaacagagca tcgcccagaa
     7621 cctctccacc ttcagtgccc atctggccaa catcgtggag atgctggcct cggccacccc
     7681 ccgatccctg ctcctgctgg acgaactggg ggcgggcacc gacccccagg aaggggccgc
     7741 cctgggcatc gccctgctcc agaccctcct ggcccgccgg actctggtgc tggccaccac
     7801 ccaccacaac cccatcaaac gcttcgccct caccacggcg ggggtggaaa ccgccagcgt
     7861 ggagtttgac cccgccaccc tctctcccac gtaccgcctc ctcatggggg tgccggggcg
     7921 gagcaacgcc ctcctcatcg cccaacgcct gggcatgccc ccggaagtgc tccacgccgc
     7981 ccgacaatcc ctggcggggg aggacgtggc tctggaggac ctcatcggag agctccagga
     8041 gaaacagggc accctggaac gggccttcga agagctggcc cgggagaaaa ccgccgtggc
     8101 ggagctccgg gagcgcttcg agcgcaaaga tgcggagctg gacgcccaaa aagaaacgat
     8161 cctcgaacgg gcggaccgca gcgccgccaa ggtcctctcc gaagcccagg aagaggcccg
     8221 ggagctgctt aagggtttgg accccgagct tctggctgcg gcccagaaag ccctgacgga
     8281 gcgccaggaa gcggtgcgcc gctccaagga agccatcgcc gtccgggcgg agcgtcggac
     8341 ggagcgccac tccgcccccg cccggggaga aaccgttcag gtggggagcg tggtgcgcct
     8401 cctcgaaagc aaggtgcggg gcaccgtgga gagcctggaa gggggcaagg ccctggtgat
     8461 ggcggggggc atgcgcatcg aagtgcccct gaagcgcctc cagctcgcca ccgaagcccc
     8521 cgaggaaaag aacgtcttcg aagaccgaac ccccggccgc cgcaagacgg accccgtcca
     8581 ctcccccctc tcccgggggg gcatttcggt gtccgccgcc tccgtcgccg tgccgagctc
     8641 catcatggtg cggggcatga ccgtggacga agccctcccc gtggtggaac gctatctgga
     8701 ccaggccttc cggcacggat accccagcgt ggtggtgatc cacggccagg gagaaggcat
     8761 cctccggcgg gaagtgcagg ccctctgcaa gcgcctcccc tacgtgctgg agttctgcct
     8821 cgggggcccc ggcgaagggg ggctcggcgt caccgtggtg accttccgga agtaggagcc
     8881 taggggaggt aaaaaggatc gatgccagtg gttgtgtttg tcaacagaaa acacccccag
     8941 gctgctcctc gaaaatgccc ccacccc
//
LOCUS       DAOOER010000069         8922 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_8226_length_8922_cov_7.246532, whole genome shotgun sequence.
ACCESSION   DAOOER010000069 DAOOER010000000
VERSION     DAOOER010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 8922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8922
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8226_length_8922_cov_7.246532"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..819)
                     /locus_tag="PL162_08070"
     CDS             complement(<1..819)
                     /locus_tag="PL162_08070"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQF92010.1"
                     /translation="MVLSTVGLLLAGIMVILGGSSPGEGSSEVAVLNPGGPALIPVAG
                     ILGGQVSGDLPLRVSVWRTQEEALGRLQGDPRLYAVLPLPLGVALGSRSDLVLLAVHE
                     WRVFSLVVSPSLPFEGLASLKGKELYMAQGRGTTLDALVRFLLLREGVDPERDLRLIY
                     APPQEIVALFRQGSLEAAALPEPFTTLCLAPGKGHVALDLQEVWQQATGGRVPVAGLF
                     AHRDRLREAPEEGAEVCRIMALSTAWGIRSPSEACAVLKKLFDTPEEPFCRGGTA"
     gene            1348..>2180
                     /locus_tag="PL162_08075"
     CDS             1348..>2180
                     /locus_tag="PL162_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HQF92011.1"
                     /translation="MEISRIRNFCIIAHVDHGKSTLADRLLEKTGTVDSRAMRDQYLD
                     LLELERERGITIKLVPVRMRYTAQDGEEYFLNLIDTPGHVDFGYEVSRSLAACEGALL
                     LVDATQGVEAQTVANAYLAVDQNLEIVPAVNKIDLPSAQPERALKELEDVVGVDTSSA
                     LLVSAKDGTGVSALLEALVARIPPPQGDPDAPLQALIFDSVYDNYRGVICYVRVVNGS
                     LRSGQQILFMATQCGDQVEEVGTFSPGMTPCTELGPGEVGYLITNIKTLEEAHVGDTV
                     T"
     assembly_gap    2181..2280
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2281..3024
                     /locus_tag="PL162_08080"
     CDS             <2281..3024
                     /locus_tag="PL162_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300822.1"
                     /note="back-translocating Elongation Factor EF4; binds to
                     the ribosome on the universally-conserved alpha-sarcin
                     loop; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HQF92012.1"
                     /translation="LHREFNVDLVATTPNVEYQIVLPGGTVLEAHRPSDFPGEGMIEE
                     IREPYIKITAFLPTEFVGKVMQLCQDRRGIFVGMDYLTPERVRLLYDLPLAEFILDFH
                     DKLKSVSRGFASLDYEHIGFRPGNLVKVDVLIGGDPVDAFSFICHADAAYYRGQAVTR
                     KLKELIPRQLFEVPLQASIGKKVIVRQNIKPLRKDVLAKCYGGDITRKRKLLEKQKEG
                     KRKMKLIGRVSVPQEAFLAFLKVGEDEEE"
     gene            3011..4249
                     /gene="hemW"
                     /locus_tag="PL162_08085"
     CDS             3011..4249
                     /gene="hemW"
                     /locus_tag="PL162_08085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HQF92013.1"
                     /translation="MRRSRGKTSGRSSFPLDLAGGEGALGDLSHRVSEEGALGGLWSL
                     YVHVPFCLRKCPYCAFPSAPMADAQEGERYLALLEQEISFWHRRWRRLPTLDTIYVGG
                     GTPTVLSPTLWARLTALLDGLFPRIPGAEVTVEANPGTLEREHLELWRRWGVTRVSVG
                     VQSLDDEALATLGRVHSSDQAREALALCVKEGFATSADLMFGLPHQTMTQWHRHLKAV
                     VELGVKHLSVYQLTLEPDTPWGACPPEGLGEGYPFYRFAQWYLPRKGLDQYEVASFSL
                     PGHRPRHNFAYWKHLPVLGLGPGAWSYVQGWRFRQEMTLKDHGQRIKSEGSPVAEEER
                     LPHPRRWREFALLALRTGQGVNLRAFRRTFGPEAFARMARDVATFFPDILTVRAGHIL
                     LTPRGWRVANRVWGALWNDD"
     gene            4275..4856
                     /locus_tag="PL162_08090"
     CDS             4275..4856
                     /locus_tag="PL162_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92014.1"
                     /translation="MQRVLFSLPDEAESFLQTVLISSARFIPSSHPRRGFHHEVGDSL
                     QGGLAFLEQHLPTEETYLMRLYASFQKDKALGCLVADGAEELFCWLQRHWVLQEGTLG
                     PLLRFPGSWKNRDWGLVGIAYTGGIVLFDDNQEEPYRQDLDEGVVASYLPLGEEADIS
                     FSPEAPTEVCLLALTKTLLGLLPEQEDEGNSST"
     gene            4991..6424
                     /gene="selA"
                     /locus_tag="PL162_08095"
     CDS             4991..6424
                     /gene="selA"
                     /locus_tag="PL162_08095"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HQF92015.1"
                     /translation="MNPAVQEVLRSIPAMDQLLAAPWVEAVARDLGHEAVKAVFSDVV
                     GEIREDLRQGRRASVDRAFLDAEAHRRCLLRCRSSLHRVVNATGVVIHTNLGRSCLHA
                     EAVEAVREAAESYSTLEYRVDRGERGHRNDHVEWLLCQVTGADAAFVVNNNAGAVLLG
                     LAALARNKQVVVSRGELVEIGGSFRVPDILAFSGAHMVEVGTTNRTHLADYEGALSPE
                     TALLLKVHPSNYRVVGFSSGVDRSDLARLAREKDLILMEDLGSGMLVDLEEQGLPGEP
                     TVRQCLRQGVDLVTFSGDKLLGGPQIGALVGRRQIIDRLRTYPLARALRVDKMTLAAF
                     EATLRLYLQGRWREIPSLAMLGRSEASLEEEARRLIPLLEAAFPEGEFAVVSAEDAVG
                     GGAYPAVAVPGIAVAVRHPRWSAGYLQQLLRQGRPSVVAGARDNLLMIHLRALLPGDG
                     ARLAEAFRFLGTAHPAVPETLMGGGAP"
     gene            6421..8361
                     /gene="selB"
                     /locus_tag="PL162_08100"
     CDS             6421..8361
                     /gene="selB"
                     /locus_tag="PL162_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HQF92016.1"
                     /translation="MTSAAEIPFVLGTAGHIDHGKTTLVKALTGVDCDRLKEEKRRGI
                     TIDLGFAPLVLPSGRVVSLVDVPGHERFIRQMVAGASGIDAVLFVVAADEGVMPQTRE
                     HLDILSLLQVQDGVVVLTKADLVDPDFLELAQEDVRHAVRGTFLEHSPMFAVSALRGD
                     SLKELRLALEDLVERVPLRNRSGGFFQPIDRAFPISGFGTVVTGTAYRGILRLGDEVD
                     LLPSGKRAKIRGLQVHGQAVEEAWAGQRVAVNLSGVGVEELQRGDVVCAAGLFRATRC
                     LDVVLRVLPSAREPLRHWQRVRLHVGTSDLLGRVSLLRSRELAPGEEDLAQLVLEEPV
                     VATMEERFVLRFYSPLVTIAGGRVLSPYGTKPRGREARKAKLRTLEALRPSTGDGDAV
                     LLALVEESGVLPLDEAALALQEKREALSARISRGQKRGLILGLRGAEGEVLVTPESLK
                     RFGEEMERYLSTFHASHPALPGAPTDQTLAKVFPRMDSRLARSVAAAWADRGGMVSEE
                     GRLRLRDFVPQDLEKLDRWLEELESESLNRGMQFLSVEEARQHLGISEKEMDLVLQQG
                     REQHRLSIMGEQMLLFKEMERRVLDLLEGMGGDLTLAAVRDASGSTRKYILPLLEHLD
                     ARGITRRVGDKRILRKRPPENT"
     gene            complement(8441..>8922)
                     /locus_tag="PL162_08105"
     CDS             complement(8441..>8922)
                     /locus_tag="PL162_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92017.1"
                     /translation="IRFFLPLWIIHRGGTLEDGGHILFAVTLAATLASLASGFLAQHL
                     GSRRLMLSMVTLAPFFLLAATWCSGPWFVVLLLLGFSALSAPLPVIGAMAQAEAPHAR
                     SMVSALVLGVTWGLGGLVTIPAGALADRWGIDAALRCIALLPWGALAFLIFGHKKPR"
BASE COUNT         1673 a         2747 c         2793 g         1609 t
ORIGIN      
        1 tgcggtccca cccctgcaaa aaggttcttc gggcgtgtca aagagttttt taagtacggc
       61 acaggcctcc gagggagatc ggatccccca agcggtggaa agggccatga tccgacaaac
      121 ttcggcccct tcttccgggg cctcgcggag gcgatcccgg tgggcaaaaa gccccgccac
      181 ggggacgcgc cccccggtgg cttgctgcca aacctcctgg agatccaagg ccacgtgtcc
      241 cttgccgggg gccaaacaca gggtggtaaa gggctcgggc aacgcggcgg cctctaggct
      301 tccctggcgg aagagggcca ctatttcctg agggggggcg tagatcagac gtaggtccct
      361 ttcgggatcc accccctccc gtaggaggag aaaacggacg agggcatcca gcgtagtccc
      421 ccgtccttgg gccatataga gctctttccc ctttagggat gccaggccct cgaagggaag
      481 cgacggggat accaccaggg aaaagacccg ccactcgtgg accgccagaa gcaccaggtc
      541 gctacgggat cccagggcca cccccagggg gagggggagg acggcgtaaa ggcgaggatc
      601 tccctggagt cgccccaggg cttcttcctg cgtcctccag acggaaaccc ggaggggaag
      661 atccccggag acctgacctc ccaagatccc cgccacggga atcagagccg ggcccccggg
      721 gttcagcacg gccacctccg aagagccttc tccagggctc gatcctccta aaatcaccat
      781 gattcccgcc aggagtagcc ccaccgtcga aagaaccata cccctcaaaa cgcccactcc
      841 ctccatcgtc gatcgatttt tgtgccatct tcgccattta cgttggaagt gcccgtcgaa
      901 agattggaaa cgatggggat cgccctccgc ccacaaaggt gccacaggtc aagccggaac
      961 tgccgcacat ctcacctttc gatcctgaaa gacaaaggac gccccatggc tcggcgaagg
     1021 aacggcacaa gggcccgtac atcccggaac tcctggggaa aaacccggcc cattatacac
     1081 ggaacccttt gccccggaac atgggaaccc cacgtcttta ttatcccgtg cccccggagg
     1141 cccgagtcct ccgccggtcc catatccatt gatgggacaa aatttccgtt ctcctctttg
     1201 cgtgtataat acgagggatg gggtcgccgc tttatcgatg gcggccgggc ctttctcggc
     1261 agagcccttt cttccgagcg gggggcgccc tgccagggcc aaaggctcct ggggaccgta
     1321 tcgatccttg tgtttggggt gaacttcttg gaaatatcgc gaatccgaaa cttttgcatc
     1381 atcgcccacg tggaccacgg aaagtccacg ctggcggatc gcctgctgga aaagaccggt
     1441 acggtggact cccgggccat gagggatcag tacctggacc ttttggaact ggagagggag
     1501 cgaggcatca ccatcaaatt ggtgccggtg cgcatgcgtt acactgccca agacggcgag
     1561 gagtattttc tcaacctcat tgacactccg ggtcacgtgg acttcgggta cgaggtttcc
     1621 cgttccctcg ccgcctgtga gggggccctc ctgctggtgg acgccaccca gggcgtagag
     1681 gcccagacag tggccaacgc ctacctggcg gtggaccaaa acctggaaat cgtgccagcg
     1741 gtaaataaga tcgaccttcc ctcggcccag cccgaacgcg ccctcaagga actggaggac
     1801 gtggtcggag tcgatacatc ctcggccctc ctcgtgagcg ccaaggatgg cacaggggtg
     1861 tctgccctcc tggaagccct ggtggcccgg atcccccctc cccagggaga tcccgacgcc
     1921 ccccttcaag ccctgatctt cgactccgtc tacgataact atcggggggt aatttgttac
     1981 gttcgcgtag ttaatggatc cctgcgatcc ggccagcaga tcctattcat ggcgacccag
     2041 tgtggcgacc aggtggaaga ggtggggacc ttttctccag ggatgacccc ttgcaccgaa
     2101 ctggggccgg gggaagtggg ttacctcatc acgaacataa agaccctcga agaggcccat
     2161 gtgggggaca cggtcaccga nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 ctgcaccggg agtttaacgt cgacctggtg gccaccaccc ccaacgtgga atatcaaatc
     2341 gtactgccgg ggggaacggt tctggaagcc caccgccctt ccgattttcc cggcgaggga
     2401 atgatcgagg agatccgcga gccctacata aagatcaccg cgttccttcc cacggagttc
     2461 gtgggcaagg tcatgcagct ttgtcaggac cgccggggga tttttgtggg catggactac
     2521 ctgactccgg agcgggtgcg tctcctctac gacctgccct tggcggagtt tatcctggat
     2581 tttcacgata agcttaaatc cgtctcccgg ggctttgcct ccctggacta cgagcacatc
     2641 ggattccgcc cgggtaacct agtaaaagtc gacgtgctca tcggcggcga tcccgtggat
     2701 gccttttcct tcatctgcca cgccgacgcg gcctactatc gggggcaggc ggtgaccaga
     2761 aagctcaagg agctgatccc caggcagctc ttcgaagtgc ccctgcaggc cagcatcggc
     2821 aaaaaggtca tcgtacgcca aaacataaaa cccctccgga aggacgtgct ggccaagtgc
     2881 tacggaggag acatcacccg caagagaaaa ctcctggaaa aacaaaaaga agggaagcgc
     2941 aagatgaagc tcatcggccg cgtttccgta ccccaggagg cctttttggc ctttctcaag
     3001 gtgggggagg atgaggagga gtaggggaaa gacctcaggc agatcctcct ttcccttgga
     3061 cctcgcgggg ggcgaagggg ctctggggga tctctcgcat cgggtctcgg aagagggcgc
     3121 cctcggggga ctctggtctt tgtacgtcca cgtgcccttc tgccttcgaa agtgccccta
     3181 ctgtgccttt cccagcgctc ccatggctga tgcccaagag ggagagcgct atctggcctt
     3241 gctggagcag gagatctcct tctggcaccg ccgatggagg aggctcccca cgctggatac
     3301 gatctacgtc ggaggaggaa cccccacggt cctgtccccg actctgtggg ctcggttaac
     3361 agcgctttta gacggccttt tcccccggat tcccggggcg gaagtcaccg tagaggctaa
     3421 tccggggacc ttggagaggg agcacttgga gctctggcgg cgctgggggg tcacccgggt
     3481 gagcgtgggg gtccaaagtt tagacgacga agccctcgcg accctgggac gggttcattc
     3541 ctcggatcag gcccgagaag ccttggctct ttgcgtcaaa gagggcttcg ccacgagcgc
     3601 cgatttgatg tttgggctgc cccaccagac catgacccag tggcatcgcc acctgaaagc
     3661 cgtggtggag ctcggcgtga agcacctgtc ggtctatcag ttgaccctgg agccggacac
     3721 tccctgggga gcttgccccc ccgaagggct aggggaaggt tacccctttt accgcttcgc
     3781 ccaatggtac ctcccccgta agggcttgga ccagtatgaa gtggccagct tctcccttcc
     3841 ggggcaccgc ccccggcaca atttcgccta ttggaaacac ttgcccgtcc tgggcctagg
     3901 tcccggggcc tggagctacg tgcaagggtg gcgatttcgc caggaaatga ccctgaaaga
     3961 ccacggccaa cggataaaat cggagggatc gccggtggcg gaagaggagc gcctccctca
     4021 ccccaggcga tggagggaat ttgcccttct ggccctccgc accggccagg gcgtcaacct
     4081 tcgggccttt cgtcgcacct tcggaccaga ggccttcgcc aggatggcac gggacgtcgc
     4141 aacctttttc cctgacatcc tgacggtgcg cgccggtcac atcctcctca ctccccgggg
     4201 atggcgggtg gcaaaccggg tgtggggcgc cctgtggaat gatgattgaa atcccacaaa
     4261 ggaggcttga gagcgtgcag cgcgtgcttt tttccctacc cgacgaagcc gaatccttcc
     4321 tccaaaccgt tttgatctcc tctgcccgtt tcatcccttc ctcccatcct cgtcgaggat
     4381 ttcaccacga ggtgggagat tccctgcagg gggggttggc cttcttggag caacacctac
     4441 cgacggagga aacctatctg atgcgcctgt acgcctcctt ccagaaggac aaagcccttg
     4501 gttgtctggt ggcggacggg gcggaggagc ttttttgctg gctccagcga cactgggtcc
     4561 ttcaggaggg caccctaggg cccctcctgc gctttcccgg atcctggaaa aatagggact
     4621 gggggctggt gggaatcgcc tatacggggg gcatcgtgct cttcgacgat aaccaagaag
     4681 agccctatcg ccaggacctc gacgaaggcg tggtggcctc ctacctcccc ctgggagagg
     4741 aagcagacat ctctttctcc ccggaggccc ccactgaggt ctgtctcctc gccctgacca
     4801 aaaccctcct gggacttttg ccggaacagg aggacgaagg gaattcttcg acgtagggag
     4861 ccccgttccc gatcttgccg gaacaggggg tcccaccctg agcctgaccc cggagcgcgg
     4921 ggttagattt tttcgatctt tacttgtcct tttcccaaaa tccatctccc ggaaaggagc
     4981 ggagcaaatc gtgaatcctg ccgtacaaga ggtcctcagg tccattccgg ccatggatca
     5041 attgctggcg gcgccctggg tggaagcagt ggcacgagac ctggggcacg aagcggtgaa
     5101 agccgttttc tccgacgtgg taggcgaaat aagggaggac ctccgtcaag ggcgacgggc
     5161 ttccgtggat cgcgccttcc tcgacgccga agctcatagg cggtgcctcc tgcggtgccg
     5221 atcatccctc caccgggtgg tcaacgccac cggggtggta attcacacta atctcggccg
     5281 ttcctgcctg cacgccgagg ctgtggaagc ggtgcgcgag gcagcggagt cctacagcac
     5341 cctggaatat cgcgtggatc ggggagagcg ggggcaccgc aacgaccacg tggagtggct
     5401 cctgtgccag gtcacggggg ccgacgcggc ctttgtggtc aacaacaatg cgggagccgt
     5461 gctcctcgga ttggcggccc tcgcccggaa caagcaggtg gtggtctccc gaggagagct
     5521 ggtggaaatc ggtggctctt tccgagtacc ggatatcctc gccttctccg gggcccacat
     5581 ggtggaggtg ggtaccacca accgcaccca cctggccgat tacgaagggg ccctatcgcc
     5641 ggaaacggcc cttctgctga aagtccaccc ttccaattac cgcgtggtgg gcttttcgtc
     5701 gggcgtggac cgaagtgacc tagctcggct ggcccgagaa aaagacctaa tactcatgga
     5761 agacctggga agtggcatgc tggtggacct cgaagagcaa ggtttgcccg gggagcccac
     5821 cgtgcgccag tgtctgcgcc aaggggtcga cctggtgacc ttttccgggg acaaactctt
     5881 gggggggccc cagattggag cccttgtggg gcgtcgtcag atcatcgatc gcctccggac
     5941 ctatcccctg gcccgggccc tgcgggtgga caaaatgact ttggctgcct tcgaggccac
     6001 tcttcgcctc tacctccagg gaaggtggag agaaattccc tccctggcca tgttgggccg
     6061 gagcgaggcg tcgttggagg aagaggcccg gcgccttatt cccctgctgg aggccgcctt
     6121 tcccgagggg gagtttgccg tggtgtccgc cgaagacgcc gtggggggcg gggcctatcc
     6181 cgccgtggcc gtgcccggca tcgccgtggc cgtgcgccac ccccggtgga gcgcgggata
     6241 cctccagcaa ctccttcgcc aagggcgccc ctccgtggtg gcgggagccc gggataacct
     6301 gctcatgatc cacctgcggg cccttctccc cggggatggg gcccgcctgg cggaggcctt
     6361 tcgcttcctg ggcacggccc atcccgccgt ccccgaaacg ttgatgggag ggggagcccc
     6421 atgacgtccg ccgcagagat ccccttcgtc ctcgggacgg cggggcatat tgaccatggt
     6481 aagaccacct tggtgaaggc cctcaccgga gtagactgcg accgcctcaa ggaagaaaag
     6541 cgccggggaa tcaccatcga cttgggcttt gcccccctgg tgctccccag cggccgggtg
     6601 gtgagcctgg tcgacgtgcc cgggcacgag cgttttatcc ggcagatggt ggcgggggcc
     6661 tcgggcatcg acgccgttct cttcgtggtg gccgccgacg aaggggtcat gccccagacc
     6721 cgggagcacc tcgacatcct ctccctcctc caggtacaag acggtgtggt ggtgctcacc
     6781 aaggccgatc tggtggatcc cgatttcctt gagctggccc aggaagacgt ccgacacgcc
     6841 gtccgaggca cgtttctgga acacagcccc atgtttgccg tttccgccct tcggggggat
     6901 tccctgaagg agcttcgcct agccctggag gatctggtgg agcgagtgcc cctccggaac
     6961 cgctccggag ggtttttcca acccatcgat cgggcctttc ccatctccgg cttcggcacc
     7021 gtcgtcaccg gcacggccta ccggggaatc ttgcggctag gggacgaggt ggatctcctc
     7081 ccctcgggta agagggccaa gattcgcggg cttcaggtcc acggccaggc cgtggaagag
     7141 gcctgggcgg gccagcgggt ggccgtgaac ctctccgggg taggggtgga ggagttgcag
     7201 cgcggcgacg tggtatgtgc cgccggactt tttcgagcca cccgatgcct ggatgtggtg
     7261 ctacgggtgt tgccctccgc ccgagagccc ctacgccact ggcagcgagt gcgccttcac
     7321 gtgggaacgt cagacctgct ggggcgggtt tccctgcttc gatcccgaga gctggccccg
     7381 ggggaggagg acctggccca actggtcctg gaagagcccg tggtggccac catggaagag
     7441 cgctttgtgt tgcggtttta cagcccattg gtgaccattg ccgggggaag ggtcctatct
     7501 ccctatggca ccaagccccg aggaagagag gctagaaaag ccaagctccg caccctggag
     7561 gcccttcgcc cgtcgacggg agacggagac gccgtccttt tggccctggt ggaagaatcc
     7621 ggcgtcctcc ccctggacga ggcggccttg gcgctgcaag aaaaaagaga ggccctatcg
     7681 gcccggattt cgcgcggaca aaagcggggc ctcatcctgg ggctgcgcgg cgcggaggga
     7741 gaggtcctgg tcactccgga gtctcttaag cgctttgggg aagagatgga acgctatctg
     7801 tcgacctttc acgcctccca cccggccctt cctggggctc ccacggacca gaccctcgcc
     7861 aaggtctttc cccgtatgga ctcccgccta gcccgtagcg tggcggctgc ctgggcggac
     7921 cgggggggca tggtgtcgga agaggggcgt cttcgcctga gggacttcgt cccccaagat
     7981 ctggaaaagt tggaccggtg gctcgaagag ctggagtctg aaagtctgaa ccgggggatg
     8041 cagtttcttt ccgtagaaga agcccgccag catctgggca tcagcgaaaa ggagatggat
     8101 ctggtgctcc agcaggggag ggagcagcac cggctctcca tcatggggga gcagatgctg
     8161 ctctttaaag agatggaacg gcgggttctc gacctcctgg agggaatggg gggagatctc
     8221 accctggcgg cggtccggga cgcctccggc agcaccagaa agtatatttt gcccctgctg
     8281 gaacacctgg acgcccgggg aatcacccgc cgggtggggg ataagaggat cctccggaaa
     8341 aggccccccg aaaacaccta gggccttcgg ggccgccctt tggccccctc ggcctcataa
     8401 aaacccctgg ggtgcaaggg atcatccgaa tggagtgaac ttaccgtggc tttttgtgtc
     8461 cgaagatcaa aaaagccagg gcgccccagg ggagaagagc tatacaccgc agggccgcat
     8521 cgatccccca gcgatcggcc aaggctccgg cgggaatggt caccagcccc ccaagacccc
     8581 aggtcactcc cagcaccagg gcgctgacca tggatcgggc gtggggagct tcggcttgag
     8641 ccatggcgcc gatgaccggc aagggagcgc tcagggcaga aaaacccagg aggaggagca
     8701 ccacaaacca cggaccggaa caccaagtgg ccgccaggag aaaaaagggc gccagggtca
     8761 ccatggagag catgaggcgg cgcgatccca ggtgctgggc cagaaagccg ctggccaacg
     8821 aggccagcgt ggcggcgagg gtgaccgcaa agaggatgtg tcccccatct tccagcgttc
     8881 ccccccgatg gatgatccag agcggaagga agaatcttat cc
//
LOCUS       DAOOER010000070         8732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_8412_length_8732_cov_9.329031, whole genome shotgun sequence.
ACCESSION   DAOOER010000070 DAOOER010000000
VERSION     DAOOER010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 8732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8732
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8412_length_8732_cov_9.329031"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PL162_08110"
     CDS             <1..834
                     /locus_tag="PL162_08110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HQF92018.1"
                     /translation="HLLKNAISHGVEDEKTRLARGKPPGGTVTVSAYSRRGSVFIEVR
                     DDGGGIDPEKVLRKAVERGLVEPGGSRSEDEVVGFIFLPGFSTAGTVDGVSGRGVGMD
                     VVKTEISRIGGRVDVHNTPGQGCAFVLKIPINMAILNGTIVDIGGIHYILPTLAVKQI
                     LQVTDDLWVSVQGRDAVVRVRDRLISVIPVEEVLGRAFGEGREVVRLALVLELDNRVK
                     ALPVRSVVGRQEIVVKPLNEAFRSLDFVSGASILGDGRVSLILDVEALFKYGGEVECR
                     A"
     gene            822..1292
                     /locus_tag="PL162_08115"
     CDS             822..1292
                     /locus_tag="PL162_08115"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HQF92019.1"
                     /translation="MQSLGGKYLTFPLGREEYGISIAKVKEIIGMMEITMVPRMPGCI
                     KGVINLRGKIIPLLDLRLKFSMEERPYTERTCIIVVEIGGRGRTMGVVVDAVSEVVTI
                     AEGEIEPPPQYGGSLDVSFLLGMGKVKGKVVMLLDIEKVLSVEELETVREMKGV"
     gene            1296..3326
                     /locus_tag="PL162_08120"
     CDS             1296..3326
                     /locus_tag="PL162_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQF92020.1"
                     /translation="MFRNAKLAVKITFGFVMVLLIAVALGGTAVYNMRQIGQDVTALA
                     EQYVPEVTTATQVERRSQQLMYEMRGYGFTEDESYHTAGAGHLTRTKEALKDARDLAD
                     RYPQLVVLRRNAEEVTQSLGTYEKLMGETVALIGKMKEIRQRMDAQAKIFMENGRAYL
                     ESQESAMEKHLFQNDTAAAKDRLVKITRANDLLDRGDEVRIVAWHAQGLRDPKIIVEA
                     LPLFDKILKAIEDIRAITVQDVNLRQLAAMEGAAKGYQGAVSDLATTWQALQEVNAQR
                     TAVGEKVSEAAQATARGGVEHIQEITKSSVEDLASSSLIMIVGLAVAVGLGMLLAFVI
                     TLSITKPVSRIAEVLSEGSSQVASASEQLSGASQQLAEDSSELAASIEETSATLQQSA
                     SMVRQNNENTKQAAMLSKQAMEAAEGGNQEMTEMMTAMGDLKKSSDEIAKIIKVIDEI
                     AFQTNILALNAAVEAARAGEAGMGFAVVAEEVRNLAQRSAQAAKDTASIIERNIALSE
                     SGVAVAVKVKGSLETIADEARKVSELVDEITAASSEQAQGIEQITKAISQMDQVTQST
                     ASNAEESASASEELSAQAESMKEAVQGLLVLVHGVGASAGLGAAPHRSSPALRGRSPQ
                     AALPGGRGKEGGAGRPQGRKGPQGTPAKGGSGHTNVVNPDDVIPLDDDLQDF"
     gene            3397..4215
                     /locus_tag="PL162_08125"
     CDS             3397..4215
                     /locus_tag="PL162_08125"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HQF92021.1"
                     /translation="MAIPEAKFSDALFRRFADLIYQRFGIVYKEEKREMLRAKISRHM
                     RREGMESYEEFFDLLTHRDSQELWMAFVDDITIHTTNFFRERNHFDHIRRHIDDILEA
                     NPRILRRRELRAWSSAASTGEEPYTLAMVLAEALGDRASFKILATDVSRGALAQGVAG
                     EYRADIRQDVEPLYIQKYFVRTAGGFAVKPELRHRVVFRLFNLADPFPFRQPLDMIFC
                     RNVMIYFSAEVQQELVDKMYQALAPGGILFIGHSESLIGKRHRFKYVQPTIYMR"
     gene            4243..4647
                     /locus_tag="PL162_08130"
     CDS             4243..4647
                     /locus_tag="PL162_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQF92022.1"
                     /translation="MGVRVLVVEDDHISAMVIQEILLSVGSADVAENGLVAVDKFRDA
                     LERDEPYDCIFLDIMMPKMDGHETLEHIRGIEQEHGIWGLDAVKVVMITALGDFDNVK
                     KSFRGQCDAYLVKPIRREKILSAVRDLGFDVP"
     gene            4675..5160
                     /locus_tag="PL162_08135"
     CDS             4675..5160
                     /locus_tag="PL162_08135"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HQF92023.1"
                     /translation="MEPREAERRFFLQPGQIFVSDEPYRVFTVLGSCVAVCAWDPRRG
                     CGGMSHQIYPRALEGAGGAAFGAQAVPHLLRLLVGLGAKREDLRAHLVGGGANPSLGS
                     TIGDENVALAEEILARARVAVVSRDVGGLLGRKVAFSTFSGEIAVYKIERIRETDWYG
                     R"
     gene            5150..6199
                     /locus_tag="PL162_08140"
     CDS             5150..6199
                     /locus_tag="PL162_08140"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HQF92024.1"
                     /translation="MADKRVRVLVVDDSALVRRIFTEELARDPEIEVVGTAPDPIIGR
                     DKIVQLNPDVLLLDVEMPRMDGLTFLEKLMAHRPMPVIVVSSLARESSETALRALDLG
                     AAEVMAKPGSSYSVKDMSEQLAEKIKAVAQMRLSRGKAAGAPKAPGEGQVPGSPAKFL
                     KTTNKVLAIGASTGGTEALKAMLSRLPANAPPTVIVQHMPQFFTKSFADRLNTLCAME
                     VKEAEDGELLRQGKALIAPGNFHMVLKRSGAQYYVEVKDGPLVFHQRPAVEVLFRSVA
                     RFAGANAVGVILTGMGKDGAQGLLEMRQAGARTIAQDEQSCVVFGMPKEAIALGAAEK
                     VLSLDRIAAAVMDLV"
     gene            6233..7720
                     /locus_tag="PL162_08145"
     CDS             6233..7720
                     /locus_tag="PL162_08145"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQF92025.1"
                     /translation="MAQPDFFTQGERILVVEDESIVALDLTGCLEGLGYVVCGHESTG
                     EGAIRAVREQAPDLVIMDIHLKDEMDGVEAAQVIVRERGCPVVFLTAYSEDHTVERAR
                     KTAPYGYILKPVHDRELDVTLRMALSKARADREMRDAKRWFEALVAALPDGVIATDRL
                     GRVQLMNPAAERLTGWKGAEASGQAVDEVAHSVPVEGPAERRPLQDSNLLSRSGAVTP
                     VNIKVQPIPGDDGKVAGFVYLVRDVSDRIHYEDTLRAATTAAEAASRAKSEFFAMMAH
                     EFRTPMTGVLGVADLLELSGLSLKQREFLEVIKTSAEGLLSILNDMLDLARIDAGRME
                     LVLSPMDPQGSLEKAAAAYAPRAQRKGLEFRYVPGPGLPRRVLGDERRLRQVVSNLLD
                     NALKFTEAGSVELRLFSRGYGGDVVTLDVEVEDTGIGIPEEGREVIFGRFEQLDRSFV
                     RRYPGTGLGLAVCQELVQLMGGEISVESTPGQGSTFRVTIPFRRG"
     gene            7780..>8732
                     /locus_tag="PL162_08150"
     CDS             7780..>8732
                     /locus_tag="PL162_08150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQF92026.1"
                     /translation="MEESPRKILIVEDEMIFAHDLKQKLERYGFRVTGVADRSDGAMA
                     LAMANPPDLVLMDVHLRGDEDGIVTAELLRAKLDVPVIFLTAYSDDVTLARATSPFGY
                     VLKPVEDRALLAAILMALHRHDAESRVQEGEAWLSGALRSIGDAVLTVDPEGKIRTLN
                     ARGEALLGVPEREAQGMPLDEALKVASSWEGGPNPLKSLENLREPFQEAERLALETRR
                     DAPVWVQIYAAPILAEGRRVGTVVVLHDLTEADAMQRRLWEREQALQKNYQLMHDSFR
                     STIRAMASIVEIRDPYTAGHQRRVAELAQALAERLELGERR"
BASE COUNT         1601 a         2636 c         3093 g         1402 t
ORIGIN      
        1 cacctgctga aaaacgccat ctcccacggc gtggaggacg aaaagacccg cctcgcccgg
       61 ggcaaacccc ccgggggcac ggtgacggtg agcgcctaca gccgccgggg gagcgtgttc
      121 atcgaggtgc gggacgacgg cgggggcatc gaccccgaga aggtcctccg caaggcggtg
      181 gagcggggat tggtggagcc cggagggagc cggagcgagg acgaagtggt gggtttcatc
      241 tttcttcccg gcttttccac ggcggggacc gtggacggag tctccgggcg cggggtgggt
      301 atggacgtgg tgaagaccga gatctcccgc atcggaggga gggtggacgt gcacaacacc
      361 ccggggcagg ggtgcgcctt cgtcctcaag atccccatca acatggccat cttgaacggc
      421 accatcgtgg atatcggcgg catccactac atcctcccca ccctggcggt gaagcagatc
      481 ctccaggtga cggacgatct ctgggtcagc gtccaggggc gcgacgccgt ggtgcgggtc
      541 cgggaccgac tgatttccgt catccccgtg gaagaggtcc tggggcgggc cttcggggag
      601 gggcgcgagg tggttcgcct ggcgttggtg ctggagctgg ataaccgggt gaaggccctt
      661 cccgtccgta gcgtggtggg gcggcaggaa atagtggtga agcctctgaa cgaagccttc
      721 cggagcctgg atttcgtctc cggggcctcg atcctcgggg atggacgggt ctctctgatc
      781 ctggacgtgg aggccctttt caagtacgga ggtgaggtcg agtgcagagc ttaggtggca
      841 agtacctgac gtttcccctg ggacgagagg agtacggcat ctccatcgcc aaggtgaaag
      901 agatcatcgg catgatggag atcaccatgg tgccccggat gcccggatgc atcaaggggg
      961 tcatcaacct ccgggggaag atcatccccc ttttggacct tcggctcaag ttctccatgg
     1021 aagaacgccc ctacaccgaa cggacgtgca tcatcgtggt ggagataggg ggccgggggc
     1081 gcaccatggg ggtggtggtg gacgcggttt ccgaagtggt gaccatcgcc gagggggaaa
     1141 tcgagccccc gcctcagtac ggcggctccc tggacgtgag ctttctgctt ggcatgggga
     1201 aggtgaaggg caaggtggtg atgctcctcg acatcgaaaa ggtgctgagc gtggaggagc
     1261 tggagaccgt gcgggaaatg aagggagtgt aggccatgtt ccggaatgca aagctcgccg
     1321 tcaagatcac ctttggtttc gtgatggtgc tcctcatcgc cgtggccctg gggggaacgg
     1381 cggtctataa catgcgtcag atcggccagg acgtcacggc ccttgccgag cagtacgtgc
     1441 cggaagtgac gacggccacc caggtggagc gccggagcca gcagctcatg tacgagatgc
     1501 gggggtatgg cttcaccgaa gacgaatcct accataccgc cggggcggga cacctcacca
     1561 ggaccaagga ggccctgaag gatgcccggg atctcgccga ccgctacccc caactggtgg
     1621 tgctccgccg caacgcggag gaagtaaccc aatccctggg gacctacgag aagctcatgg
     1681 gagagaccgt ggccctcatc ggtaaaatga aagagatccg ccagcgcatg gacgcccagg
     1741 cgaagatctt catggaaaac ggccgggcct acctggaaag ccaggaaagc gccatggaaa
     1801 agcacctctt ccagaacgac acggccgccg ccaaggaccg gctggtgaag atcacccggg
     1861 cgaacgacct cctcgaccgg ggagacgaag tgcggatcgt cgcgtggcac gcccaggggc
     1921 tccgggatcc caagatcatc gtcgaggccc tgccgctctt cgacaagatc cttaaggcca
     1981 tcgaggatat ccgggccatc accgtccagg acgtgaacct ccgccaactc gccgccatgg
     2041 aaggcgccgc caaggggtac cagggggccg tgtcggatct ggccaccacc tggcaggccc
     2101 tccaagaagt caacgcccaa cggacggcgg tgggagagaa ggtgtccgag gcggcccagg
     2161 ccacggcccg gggaggggtg gagcacatcc aggaaatcac caagtcctcc gtggaggatc
     2221 tggcctcgtc gtctctgatc atgatcgtgg ggctggcggt ggcggtgggg ctggggatgc
     2281 ttttggcctt cgtcatcacc ctatccatca ccaagccggt gagccgcatc gccgaggtcc
     2341 tctccgaggg ttcgtcccag gtggcctccg cctcggagca gctctcgggg gccagccagc
     2401 agctcgccga ggacagttcg gagctcgccg cctccatcga ggaaacctcg gcgacgctcc
     2461 agcaatcggc ctccatggtg cgccagaaca acgagaacac caagcaggcc gccatgctct
     2521 ccaagcaggc catggaggcc gccgaggggg gcaaccagga gatgaccgag atgatgaccg
     2581 ccatggggga tctcaagaag tccagcgacg agatcgccaa gatcatcaaa gtcatcgacg
     2641 aaatcgcctt ccagaccaac atcctggcct tgaacgccgc cgtggaggcg gcccgggcgg
     2701 gagaggcggg gatgggcttt gccgtggtgg cggaggaggt gcgaaatctg gcccagcgca
     2761 gcgcccaggc ggccaaagac accgcctcca tcatcgagcg caacatcgcc ctctcggagt
     2821 cgggggtggc cgtggcggtg aaggtaaagg gctccctgga aaccatcgcc gacgaggccc
     2881 gcaaggtgag cgagctggtg gacgagatca cggcggccag ctcggagcaa gcccagggga
     2941 tcgagcagat caccaaggcc atctcccaga tggatcaggt gacccagagc accgcctcca
     3001 acgccgagga gagcgcctcg gcctccgagg aactttcggc ccaggccgag agcatgaagg
     3061 aagcggtgca ggggctcttg gtcctggtgc acggcgtcgg ggcgtcggcg ggattggggg
     3121 cggcccccca tcgttcttcg ccggccctca gggggcgttc gccccaggcg gcccttccgg
     3181 gagggcgagg caaagagggg ggagcggggc gaccccaggg gcgaaagggc ccgcaaggga
     3241 ccccggccaa ggggggaagc ggccacacca atgtggtgaa cccggacgac gtcatccccc
     3301 tggacgatga cctccaggac ttctaggccc gctgcgggga gtgaggggtg aagaactaag
     3361 aggttgaggg gagcgcgaga gaagaggtga gtccccgtgg ccatccccga agcgaagttt
     3421 tccgacgccc tgtttcgccg ctttgccgat ctgatctacc agcgctttgg catcgtctac
     3481 aaggaagaaa agcgggagat gttgcgggct aagatctcca ggcacatgcg ccgggagggg
     3541 atggaatcct acgaggagtt cttcgacctc ttgacccacc gagattccca agagctgtgg
     3601 atggccttcg tggacgacat caccatccac accacgaact tttttcgcga gcggaaccac
     3661 ttcgaccaca tccgccgcca tatcgacgac attctggaag ccaatccccg catcctgagg
     3721 cggcgggagc tccgggcctg gagctccgcc gcctccaccg gggaggaacc ctacaccctg
     3781 gccatggtgt tggcggaggc cctgggagac cgggcgtcct tcaagatcct ggccaccgac
     3841 gtgagccggg gggctttggc ccagggcgtg gcgggggagt accgggccga catccgacag
     3901 gacgtggagc ccctttacat ccaaaagtac tttgtgcgca ccgccggggg ctttgcggtg
     3961 aagccggagc tccgccatcg ggtggtcttt cgcctcttca acctggcgga cccctttccc
     4021 tttcgccaac ccctggacat gatcttctgc cgcaacgtca tgatctactt ctcggcggag
     4081 gtgcagcagg agctggtgga caagatgtac caggccctgg cccccggagg gattctcttc
     4141 atcggccact ccgagagcct catagggaaa aggcatcgct tcaagtacgt tcaacccacc
     4201 atatacatgc gatgatcgat gggttttcag gagaggaggg ggatgggggt gcgcgtcctg
     4261 gtggtggaag acgatcacat cagcgccatg gtgattcagg agatcctgct ctcggtgggg
     4321 agcgccgacg tggcggaaaa cggcctcgtg gcggtggaca agttccgcga cgccctggag
     4381 cgggacgagc cctacgactg catctttttg gacatcatga tgcccaagat ggatggccac
     4441 gagaccctag agcacattcg gggcatcgaa caggagcacg gcatctgggg gttggatgcg
     4501 gtgaaggtgg tgatgatcac ggccctgggg gatttcgata acgtaaaaaa atccttccgg
     4561 gggcagtgcg acgcctacct ggtgaagccc attcggcggg aaaagatcct ttcggcggtg
     4621 cgggatctgg gatttgacgt tccttaagga tctagccccc cgggtggtgc ggtgatggag
     4681 cctcgcgaag cggaacggag gtttttcctc cagccggggc agattttcgt ctcggacgag
     4741 ccctaccggg tgttcaccgt gctcggctcc tgcgtggcgg tgtgcgcctg ggatccccgg
     4801 cgggggtgcg ggggtatgag ccaccagatc tacccccggg ccctggaggg ggcggggggg
     4861 gcggccttcg gagcccaggc ggtgccccac ctgttgcgtc ttttggtggg gctcggggcg
     4921 aagcgggagg atctccgggc ccacctggtg ggaggggggg cgaacccatc gctcggcagc
     4981 accatcggcg acgaaaacgt ggccctggcg gaggagatcc tggcccgggc ccgggtggcg
     5041 gtggtctccc gggacgtggg gggactgcta ggaagaaaag tggccttttc gaccttttcg
     5101 ggggagatcg ccgtgtacaa aatcgaacgc atcagggaga cggattggta tggcagataa
     5161 gcgcgtccgc gtcctcgtcg tcgacgattc ggcgttggtg cgccggatct tcaccgagga
     5221 gctcgcccgg gatccggaga tcgaggtggt gggcaccgcc cccgacccca tcataggccg
     5281 ggacaagatc gtccagctca accccgacgt gctcctgctg gacgtggaga tgccccgcat
     5341 ggacggcctc accttcctgg aaaagctcat ggctcaccgc cccatgcccg tcatcgtggt
     5401 gagctccttg gcccgggaga gctccgagac ggcccttcgg gccctggatc tgggggcggc
     5461 ggaggtcatg gccaaaccgg ggtcgtccta ttcggtgaag gacatgagcg agcagctcgc
     5521 cgagaagatc aaggccgtgg cccagatgcg cctttcccgg gggaaggccg ccggcgcccc
     5581 caaggccccg ggggagggac aggtcccggg ttcgccggcg aagtttctga agaccaccaa
     5641 taaggtcttg gccatcgggg cctccacggg gggcaccgag gccctcaagg ccatgctctc
     5701 ccggctgccc gccaatgccc cccccacggt gatcgtgcag cacatgccgc agtttttcac
     5761 caaatccttc gccgatcggc tcaacaccct ctgcgccatg gaggtgaagg aggccgaaga
     5821 cggggagctc ctgcgccaag gcaaggccct catcgccccg gggaactttc acatggtcct
     5881 caaaaggagc ggggcccagt actacgtgga ggtcaaggat ggccccctgg tcttccacca
     5941 gcgtccggcg gtggaggtgc tctttcgctc cgtggcgcgc tttgccggag ccaacgccgt
     6001 gggagtgatc ctcacgggga tgggcaagga cggggcccag gggctgctgg agatgcgcca
     6061 ggcgggggct cggaccatcg cccaggacga gcagagctgc gtggtctttg gcatgcccaa
     6121 ggaggccatc gccctggggg cggcggaaaa ggtcctttcc ctggaccgca tcgccgccgc
     6181 cgtgatggat ctggtctagg ccaggtcatg aagaaagcgg ggtgatcgca cgatggcgca
     6241 gccagatttc tttacccaag gggagcgcat cctggtggtg gaagacgaga gcattgtggc
     6301 cctggacctg acggggtgcc tcgaggggct gggttacgtc gtctgcggcc acgagtccac
     6361 gggggagggg gcgatccggg cggtgcggga acaggccccg gatctggtca tcatggatat
     6421 ccacctcaag gatgaaatgg acggcgtcga ggccgcccag gtcatcgtcc gggagagggg
     6481 ttgtcccgtg gtctttctca ccgcctactc cgaggatcac acggtggagc gggcccggaa
     6541 gacggcgccc tacggctaca tcctcaagcc cgtgcacgat cgggagctcg acgtgaccct
     6601 ccgcatggcc ctttccaagg cccgggcgga ccgggagatg cgggacgcca agaggtggtt
     6661 cgaagccctc gtggccgccc tgcccgacgg cgtgatcgcc acggatcgtt tggggcgggt
     6721 gcagctcatg aacccggcgg cggagcgcct cacgggatgg aaaggggcgg aggcctcggg
     6781 gcaggccgtg gacgaggtgg cccacagcgt ccccgtggag gggcccgcag agcgtcgtcc
     6841 ccttcaggac agcaacctcc tctcccgtag cggcgccgtg accccggtga acatcaaggt
     6901 gcaacccatc ccgggggacg acggaaaggt cgcggggttc gtctacctgg tgcgggacgt
     6961 ctccgaccgc atccactacg aagataccct gcgggccgcc acgacggcgg cggaggctgc
     7021 gtcccgggcc aagagcgagt tcttcgccat gatggcccat gagtttcgca cccccatgac
     7081 gggggtgctg ggcgtggccg acctgctgga gctctcgggg ctttctctca agcaacggga
     7141 gttcctggag gtcatcaaga cctcggcgga ggggcttttg tccatcctga acgacatgtt
     7201 ggacctcgcc cggatcgacg cgggaaggat ggagctggtc ctttccccca tggaccccca
     7261 gggatccttg gagaaggcgg cggcggccta cgccccccgg gcccagcgca aggggctgga
     7321 gttccgctat gtcccggggc ccggccttcc ccgccgagtc ctgggggacg agagacgcct
     7381 gcgtcaagtg gtgtccaacc tcctggacaa cgccctcaag ttcaccgagg ccgggagcgt
     7441 ggagctccgc ctcttttccc ggggatatgg gggagacgtg gtgaccctgg acgtggaggt
     7501 ggaagacacg gggatcggca tcccggagga ggggcgggaa gtcatcttcg gccgcttcga
     7561 gcagttggac cgctccttcg tgcggcgcta ccccggcacg gggttggggc tggcggtgtg
     7621 tcaggagctg gtgcagctca tggggggaga gatttccgtg gagagcaccc cgggccaggg
     7681 gagcaccttt cgggtgacga tccccttccg gaggggatga ggcgccgccg gggcttcggg
     7741 acccgaagag atctccggcg gggagagggg ggaggctcca tggaggaaag ccctagaaag
     7801 atcctcatcg tggaagacga gatgatcttt gcccatgacc tgaaacagaa actggagcgg
     7861 tacggctttc gggtgacggg ggtggcggac cggagcgatg gggcgatggc cctggcgatg
     7921 gccaaccccc ccgatctggt cctcatggac gtccacctcc ggggggatga ggacggcatc
     7981 gtcacggcgg agctcctccg ggcgaagctc gacgtgccgg tgatcttcct caccgcctac
     8041 tcggacgacg tcaccctggc ccgggccacg agccccttcg gctacgtgct caagcccgtg
     8101 gaggatcggg ccttgctggc ggccatcctc atggcgctcc atcgccacga cgcggagtcc
     8161 cgggtccagg agggggaagc gtggctttcg ggggccctgc ggagcatcgg tgacgcagtg
     8221 ctcaccgtag atcccgaggg gaaaattcgc accctcaacg cccgggggga agccctcctg
     8281 ggggtgccgg agcgcgaggc ccaggggatg cccctggacg aagccctcaa ggtggcttct
     8341 tcctgggagg ggggccccaa ccctcttaaa agtctggaaa atcttcgaga accctttcaa
     8401 gaggccgagc gcctggccct ggagacccga agggacgccc cggtttgggt gcagatctac
     8461 gccgcgccga tcctggcgga gggccggcgg gtgggcaccg tggtggtatt gcatgatctc
     8521 accgaggccg atgccatgca acggcgcctc tgggagcgcg aacaggccct acagaagaac
     8581 taccaattga tgcacgactc cttccggagc accatccggg ccatggccag catcgtggag
     8641 atccgcgatc cctacacggc ggggcaccag cgccgggtgg cggagctggc ccaggccctg
     8701 gcggagcgcc tggagctggg ggaaaggcgc ag
//
LOCUS       DAOOER010000071         8719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_8433_length_8719_cov_8.964104, whole genome shotgun sequence.
ACCESSION   DAOOER010000071 DAOOER010000000
VERSION     DAOOER010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 8719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8719
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8433_length_8719_cov_8.964104"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="PL162_08155"
     CDS             complement(<1..269)
                     /locus_tag="PL162_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92027.1"
                     /translation="MAKKYQTRHPGFRFFYALFPLLALGCLGAVGYAGYSFVRDLRPP
                     PRVEDPRSTPTPLPPRESDGGEEALLLAHPGETTIPEKAPTPSLPA"
     gene            714..1622
                     /locus_tag="PL162_08160"
     CDS             714..1622
                     /locus_tag="PL162_08160"
                     /inference="COORDINATES: protein motif:HMM:NF016292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
                     /protein_id="HQF92028.1"
                     /translation="MVESYSQEYAWDKDYCDALREKLGGAHTLEFFELDTKTLPAEEH
                     AKMGDRAWAKIEETKPDLVVLGDDAALKFLGPRLAKAEIPTVFLGINGNPRNSFEDHR
                     LPANFTGVLERPIYKRFILLVGEMVKGAKDCLILFDTNITSEIVKEEVFKGREREQVG
                     EITTDLRLLKTFDEWKAAVTEAGGKYDFIVTGLYQAVRDDKDQSVPADDVITWVSAHS
                     PVPVFGFWDFSVGKDKTIGGLVLDGREQGLKAADIILQILGGKAAVDVPPVTESRGKS
                     LFSRGQLEKWQLTIPESIKEGAILVD"
     gene            1890..>3607
                     /locus_tag="PL162_08165"
     CDS             1890..>3607
                     /locus_tag="PL162_08165"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQF92029.1"
                     /translation="MNDLPKEGLRALVAQEGMALLGDPERARALLEERFPQFPEEIEA
                     LVRVLEEGVMESLAQGANLSAQAPELEARTGLSLEQILWGAQGWVDALPPAPPSGEDA
                     EGPSGTLAPEAPPEAPAPGDSLSLEEFFRRPPLSREPSQEERNLALLGLSGDRGRLFT
                     APWEGARPLETPPLPRGLVALLQSSLSPLGGGEERQAPLSEPEEPRGRGGAEKGEGEV
                     SPEPEVPVPLAETLPPPAEALKEEPWFAFPEDEEESPGRGGFPSKEPEDMAPPIREEE
                     VEALAFSEAPEKEDEGPEGDRAVPRRTLATFPPPLPPREFHPAFSEEGPSSEEGDRGV
                     LIADDRAEVESLLGKRPPRRRLSPGALRLASFLVLGVVVGSGVWFWWHRPQRFFDQGQ
                     ELFQRKDFALATASFQRGLEEDPRNADALFMLGESFRALGSADEALEAYEKVLRLTSG
                     DAMLFVGMGEAHLVRGEPILAVKAFDLALSLVPTFSEARKGLGLAYLARGDYPKAREA
                     LEKALEGRRDDLPLMKTLAAVRRMMEDPGGARALYDEVLLLTSDDQEAREGRAATLRE
                     EEELRRS"
     assembly_gap    3608..3707
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3708..5165
                     /locus_tag="PL162_08170"
     CDS             <3708..5165
                     /locus_tag="PL162_08170"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQF92030.1"
                     /translation="LFRHEQLMLFSAPPVLRGEEMLREGNPGGALVLFREALSADAKD
                     PRSWKGLGRALLTLGSPDEALEAFRRGEGLVSGDADLARGIKEAREARELEKRRERAA
                     ELGRQGEKEEARGKYRQAVKLFRQALDLASDDQSALRGAARVTALQGLHGDAAAYYRR
                     LLRVVSGDQEARKELARLERLGGARVTPGAGHPSEASRLAELAPGTSPHPTPSSRAMP
                     LAGPVTPARERLGELHARKGGALAAQGAYAEAAREYRRALDLSPWRGAYRNNLGWALA
                     RMGVTEGALGEFRQAVLQDGDRGDVQYNYGWVLARQGEVPKAARALVASLCLDDGSDG
                     AYLVIREPFFSSRDVERALSGDARPFVQAAQAAAALRPEDEVRYWNVALAVYEAGRRS
                     PSLAVSPTALERIFLGHAWMVQDPARARQELDQEEEGEGWMGSLRLTLLGQAASREGR
                     PDRAADAYRRALELDPRNDAARAAWQALGRLSETP"
     gene            5444..6553
                     /locus_tag="PL162_08175"
     CDS             5444..6553
                     /locus_tag="PL162_08175"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT domain-containing protein"
                     /protein_id="HQF92031.1"
                     /translation="MDAQSIGEWKDRYIRKHDLYVGYTERLKHLVQDLLENLGLELFH
                     IESSTKSIERFVEEISEPGAEYADPFAEMADLSCVRVILYFAEDLDKVAAVVEEEFQT
                     VREESVPFDDMQDPDAYGYRSIEYSLYLSRARSDLREWQKYASLKARIQVRTILQNAW
                     SAITEKFPAGAVFQPKSLVKRKLGRMSALLEEADEGFLTLWEFVKQGPFSSGPRGVSA
                     PSRTGGAPAGSPLGSVSLTTDTLETFFRTSPERLDFWVGVAREIGLPLVEPGADYLRK
                     SLENLALILVAADIATPAALDAFLSAMATESRGRQHLLTIYRSFEGEIAGWRIDAYSV
                     LFLLVLNAKWDVLQHKDLVAYGIKTATDRIKGLSR"
     gene            6688..7866
                     /locus_tag="PL162_08180"
     CDS             6688..7866
                     /locus_tag="PL162_08180"
                     /inference="COORDINATES: protein motif:HMM:NF019585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HQF92032.1"
                     /translation="MEKLASDTRIREVRPGEGKTFLLTLEDNPFRGAGGGQPGDSGEL
                     RGEGFLAEVKDGREEGGEVWLEATCRRGTPRPGLAVEARVDGPRRALLSRHHSGEHLL
                     SRILEDAHPGLAVHKVAVGEESTSVYFTYDGEVDWAMLFSAEDRAREVVASDLPVTIQ
                     EYSREEGQKIPRLKVNWDRVHSPTLRVVRIGEDLDVIACSGSHVSSTGEIGEIFITGY
                     HGAPPEWSFTFTVCGRSRERDYGKILRRVLRTVGCEPGQLEKVFERLQGEKGALQKTL
                     DKVRPLVSLPWERHALGEYTLDVAVVAALPPEMATPAVKRHGETFPRGVALVLLPGTA
                     DDRVPFVLARGAEVAMDLRRAIKDPELGARGGGAPEWVSGVVRGASVAPWLRALGEAL
                     "
     gene            7863..>8719
                     /locus_tag="PL162_08185"
     CDS             7863..>8719
                     /locus_tag="PL162_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HQF92033.1"
                     /translation="MTPIPDLHRLFHPRSVALIGASEKLDSISGRPLHLLKEHGFPGR
                     VYPVNPRHREIDGTPCFPDVASLPETPDVVLVGVRAALVPAVLEACAVRGVPFGVIFS
                     SGFAESDGAEEERRMVEIARGGGMRLLGPNCQGLANFREDIPLTFSSSLLGRTPLPGG
                     VAYVSQSGAFGFASAAQAMDRGVRFHSVVTTGNQCDLDALDVGRHLLRDPEVRALLIY
                     LEGIRDGERFVALAEEARERGIPLGVLKVGKSASSREAVQSHTAALAGDPEVWEAIFR
                     QYGIFPLEDA"
BASE COUNT         1410 a         2745 c         3060 g         1404 t
ORIGIN      
        1 gcgggaaggg atggcgtggg ggccttctcg ggaatggtcg tctccccggg atgggcgagg
       61 aggagggcct cctccccccc gtcgctctcc cgggggggga gcggggtggg ggtggatcgg
      121 gggtcctcga cgcggggcgg gggacgcagg tcccgcacga aggaatagcc cgcgtagccc
      181 acggccccga ggcaccccag ggccagcagg gggaagaggg cgtaaaaaaa gcggaaaccg
      241 ggatggcggg tctgatattt tttggccaag gctcttctcc taaaaattta tgtccttcgc
      301 cccgggcccc ctgagggccc ccggggcgtc ttctaccttt atgccggata ccccgccccg
      361 ctgtcaaggg aagcttgcgg gattcgcccc ttcggcccca tgcgaagggg gccatcccgg
      421 tcgtcgggcg aagggggatg ggccctcccc ggggcgcctt accctcccgg aggccatgac
      481 ccccttcccg ggcggcgggg gcgaaggggc gccctccggt cacgccgccc gggcgggcga
      541 ttgcccccca tgccctattg tgatagaatg tcacccgatg ccctggggca aagttagatc
      601 gaggggagtg gtttagcatg gcgcagagta aagctcttcg gcgaattggg gcggtttttc
      661 tgactctttt gctcttgtgt ggcgcggccg gagcagaagg taaacgtatt ctggtggtag
      721 agagttatag tcaggagtac gcctgggaca aggactactg cgacgccctg agggaaaagc
      781 tcggcggtgc ccatacgctg gagttctttg agctcgacac taagaccctg cccgcagagg
      841 aacacgcgaa gatgggggat agggcctggg caaagatcga agagacgaag cccgacctcg
      901 tggtgctggg agacgatgcg gccctgaagt ttctcggccc tcgcctggcc aaggccgaga
      961 tccccacggt ttttctgggg atcaacggga acccgcgcaa cagcttcgag gaccatcgtc
     1021 tccccgccaa tttcacggga gtgctggaac gccccattta caagcgtttc atccttctcg
     1081 tgggagagat ggtcaaggga gccaaggatt gcctgatcct cttcgatacg aacatcacct
     1141 cggagatcgt gaaggaggag gtcttcaagg gcagggagcg cgagcaggtg ggggagatca
     1201 ccaccgacct gaggttgctc aagaccttcg acgaatggaa ggccgccgtg accgaggccg
     1261 gaggaaagta cgacttcatc gtcaccgggc tctaccaggc ggtgcgtgac gacaaggatc
     1321 agtcggtccc cgcagacgac gtgatcacct gggtgtcggc ccattcgccg gttcccgttt
     1381 tcggtttttg ggatttctcc gtgggtaagg acaagacgat cggcggtctg gtgctggacg
     1441 gccgagagca ggggctcaag gccgcagaca tcatcctgca gatcctgggg ggcaaggctg
     1501 cggtggacgt gcctccggtg acggaaagcc ggggcaagag cctctttagc cgggggcaac
     1561 tggagaagtg gcagctgacg atccctgaat ccatcaaaga aggggcgatc ctcgtcgatt
     1621 gaccgcgtcc ccgaagggta aaacgacaag gaaatccctt tgggcgaaag gggcgtcccc
     1681 cgccccttcg cccttttacc tgctcccctt cgacgacggg gcatgttgaa ggggggaccc
     1741 ctccatttaa agatgatccg tggtatgata tcccctggcc tcaggggcca ggttaggtgt
     1801 gccttttttc actccagggg cttccccgga gggggtcccc ccgcgagggt ttttgaattt
     1861 tccgccgagg tcttggaggt gctggagcga tgaacgatct tccgaaggag gggttgcgcg
     1921 ccctggtggc gcaagaaggc atggccctct tgggcgatcc ggagcgggct cgcgctctcc
     1981 tggaggaacg tttcccccag ttcccggaag aaatcgaggc cctggtccgg gtcttggagg
     2041 aaggggtcat ggaatccttg gcccaagggg cgaacctttc ggcccaggcc ccggagctgg
     2101 aggcccgcac gggcctatcc ctggaacaga tcctctgggg cgcgcaggga tgggtggatg
     2161 ccctgccccc ggcgccgccc tcgggagaag atgccgaggg accctccggg acccttgccc
     2221 cggaagcccc cccggaggcc cccgcccccg gggattcgct ctccctggag gagttctttc
     2281 gccgcccccc cctttcccga gagccctccc aggaggagcg gaacctggcc ctcctaggcc
     2341 tgtcggggga tcgggggcgc ctcttcacgg cgccctggga gggggcacgc cctttggaga
     2401 cccctcccct tccccggggg ctggtggccc tcctgcaatc ttccctttcg cctctgggag
     2461 gaggggaaga aaggcaagcc cccctctccg agcccgaaga gccccggggg agaggtggcg
     2521 ccgaaaaagg ggagggcgag gtttcgccgg agcccgaagt cccggtcccc ttggcggaga
     2581 ccctgccgcc tccggcggag gccttgaaag aagaaccctg gttcgccttt cccgaagacg
     2641 aagaagagag cccggggcgg gggggctttc cctcgaaaga acccgaagat atggcccctc
     2701 cgatccgaga ggaagaggtc gaggccctgg ccttttcgga ggcccccgag aaagaagacg
     2761 aaggccccga gggggaccgg gcggtacccc ggcgcaccct ggcgaccttt cccccgccct
     2821 tgcccccccg ggagttccat ccggccttct ccgaggaggg gccctcttcg gaagaggggg
     2881 atcgaggggt gctcatcgcc gacgaccggg cggaggtgga aagccttttg gggaagaggc
     2941 cgccccggcg gcgcctgtcc cccggggcgc tccgcctggc ctccttcctc gtcctggggg
     3001 tggtggtggg gagcggggtt tggttctggt ggcatcgccc tcaacgattt tttgaccagg
     3061 ggcaagagct ctttcagcgc aaggactttg ccctggcgac ggcctctttt cagcgggggc
     3121 tggaggaaga tccccggaac gccgacgcgc tcttcatgct gggggagagc tttcgcgccc
     3181 tggggagcgc cgacgaggcc ctggaggcct acgaaaaggt cctgcgtctg acctcggggg
     3241 acgccatgct cttcgtgggc atgggagagg cccacctggt ccggggggag cccatcctcg
     3301 ccgtgaaggc cttcgatctg gccctgtccc tggtgccgac cttctccgag gctcgcaagg
     3361 ggttggggtt agcctatctg gcccggggcg attaccccaa ggcccgggaa gccctggaaa
     3421 aggccctgga gggtcggcgg gacgacctgc ccctgatgaa gaccctggcg gcggtgcgcc
     3481 ggatgatgga agaccccggg ggggcccggg ccctttacga cgaggtcttg ctcctgacgt
     3541 cggacgacca ggaggcccgg gaggggcggg cggcgaccct ccgggaggaa gaagagctcc
     3601 gccggtcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctc ttccgccacg
     3721 aacagctcat gctcttcagt gccccccccg tacttcgggg cgaagagatg ctccgggagg
     3781 gaaatcccgg cggggccctc gtcctcttcc gggaggccct gagcgccgac gcaaaggacc
     3841 cccggagttg gaagggcctg ggccgagccc tcctgaccct gggcagcccc gacgaggccc
     3901 tggaggcctt tcgccgggga gaggggctcg tctccgggga cgcggacctt gcccggggga
     3961 ttaaagaggc ccgagaggcg cgggagctcg agaagcgacg ggaaagggcc gccgaattgg
     4021 ggcgccaggg ggagaaagaa gaggcccggg gcaagtaccg ccaggcggtg aagctcttcc
     4081 gccaggccct cgacctggcc tccgacgacc aaagcgccct gcggggggcg gcccgggtga
     4141 ccgcccttca agggcttcac ggcgatgcgg cggcgtacta tcgacgcctc ctccgagtgg
     4201 tgtcgggcga ccaagaagcc cggaaagagc tggcccgtct ggaacgtcta gggggcgccc
     4261 gggtgactcc gggggcgggc catccatcgg aggcgtcccg ccttgcggag cttgccccgg
     4321 ggacttcgcc ccaccccacc ccctcctccc gcgccatgcc cctggcgggc cccgtgaccc
     4381 cggcccggga gaggctcggg gagctccacg cccgaaaggg aggggccctg gcggcccagg
     4441 gggcctatgc ggaggcggcc cgggagtacc gtcgggccct ggacctttcc ccctggcggg
     4501 gggcctaccg aaacaacctg gggtgggccc tggctcgcat gggggtgacc gaaggggccc
     4561 tgggggagtt ccgccaggcg gtccttcagg acggagaccg gggggatgtc cagtacaact
     4621 acggatgggt cctggcccgc cagggggaag tccctaaggc ggcgcgggcc ttggtcgcga
     4681 gtctctgcct cgacgatggc tcggacggcg cctatctggt gattcgggaa cccttcttct
     4741 cctctcggga cgtggagagg gccctatccg gggacgcccg ccccttcgtc caggccgccc
     4801 aggccgcagc ggccctgcgc cccgaggatg aagtgcgcta ctggaatgtg gccctggcgg
     4861 tctacgaggc gggccggcgg tcgccctcgc tggcggtctc tcccaccgcc ctggaacgga
     4921 tctttttggg gcacgcctgg atggtccagg accccgcccg ggcgcggcag gagctcgacc
     4981 aggaagaaga aggggaggga tggatgggct ccctccgcct gacccttctg gggcaggcgg
     5041 catcccggga gggacgcccc gaccgggccg ccgacgccta tcgccgggcc ctggagctcg
     5101 acccccgaaa cgacgcggct cgcgccgcct ggcaggcctt gggcaggctc tccgagaccc
     5161 cttagggggc cccggccgag cgggcaagaa ggcaagaaag gtaaggcggg acgggctttc
     5221 gcacgaccgg agaaaaggcc cggagggcga ggggagcccc ttcggactcc gagggagaag
     5281 ctccccgggg taagcccctt ctgtggttta tgaggaaagt gtcccggccc ccttggcaaa
     5341 agggagcctc tttgtgtaaa ctggtccagg agtctccttc atcctttggt cgtgtttgct
     5401 cggtttcgct ggtggcataa ttcctgcggg tggtgatggt cgcatggatg ctcaaagcat
     5461 cggggaatgg aaagatcgat atatccggaa gcacgacctg tatgtgggct acacggagcg
     5521 cctgaagcac ctcgtacagg atctgctgga gaacctcggg ctggagctct ttcacatcga
     5581 aagcagcacc aagagcatcg agcgcttcgt ggaggaaata agcgagcccg gggccgagta
     5641 cgccgacccc ttcgccgaga tggcggacct ttcctgcgtg cgggtgatcc tctactttgc
     5701 cgaggacctg gacaaggtgg cggcggtggt ggaagaggag ttccagaccg tgcgcgagga
     5761 gtccgtgccc tttgacgaca tgcaagaccc cgatgcctac ggctatcgct ccatcgaata
     5821 ctccctctac ctctctcggg cccggagcga cctccgggag tggcagaagt acgcctccct
     5881 caaggctcga atccaggtgc ggaccatcct acaaaatgcc tggtccgcca tcacggagaa
     5941 gttccctgcg ggggcggtgt tccagcccaa gagcctggtg aagcgcaagc tggggcgcat
     6001 gagcgccctg ctggaggagg ccgacgaagg cttcctcacc ctttgggagt tcgtgaaaca
     6061 ggggcccttc tcttccggcc cccggggcgt ttccgccccc tcccgaacgg ggggcgcccc
     6121 ggcgggatcc cccctgggga gcgtttccct cacgacggac accctggaga ccttcttccg
     6181 tacctcaccg gagaggctcg acttctgggt gggggtggcc cgggagatcg gccttcccct
     6241 ggtggagccc ggggcggatt acctgcgcaa gagcctggag aacctggcgc tgatcctggt
     6301 ggcggcggac atcgccaccc ccgccgcgct ggacgccttc ctgagcgcca tggcgacgga
     6361 atcccgggga cggcagcacc ttctcaccat ctatcgctcc ttcgagggag agatcgcggg
     6421 atggcgcatc gacgcctact ccgtcctgtt tctcctggtg ctcaacgcca agtgggacgt
     6481 gttgcagcac aaggacctgg tggcctacgg catcaaaacc gccacggacc gcatcaaggg
     6541 cctgtcacgc tgatcggaac gcccgagccg tttccgaaaa aaaattcccc aagatcccgg
     6601 ggcgggacaa tcgcatcgag gggccttccc ctcgatgttt gttttcttta agatcccgtc
     6661 cggacgggag agaggaggcc aatcgtcgtg gagaagttgg catcggacac aaggatccga
     6721 gaggtacgcc ccggagaggg caaaaccttc cttttgacgc tggaggacaa ccccttccgg
     6781 ggagcgggag ggggccaacc cggagactcg ggggagcttc ggggagaggg cttcctggcg
     6841 gaggtgaaag acggccggga ggaggggggg gaggtctggc tcgaggcgac ctgtcgccgg
     6901 gggacccccc ggccggggct cgccgtggaa gcccgggtgg acgggccaag gcgggccctc
     6961 ctgtcgcggc accacagcgg tgaacacctc ctctcccgca tcctggagga cgcccacccc
     7021 gggctggcgg tgcacaaggt ggcggtgggg gaggagagca cctcggtgta cttcacctac
     7081 gacggcgaag tggattgggc catgctcttt tccgccgagg accgggcccg cgaggtggtg
     7141 gcgtcggatc tccccgtgac gatccaggag tactcccggg aggaggggca aaagattccc
     7201 cggctcaagg tgaactggga ccgggtccac tcccccaccc tccgggtggt gcgcatcggc
     7261 gaggacctgg acgtcatcgc ctgctccggt agccacgtct cctccacggg ggagatcggg
     7321 gaaattttca tcacgggata ccatggcgcc cccccggagt ggtcttttac cttcaccgtc
     7381 tgcggcaggt ctcgggagcg ggactatggc aagatcctcc gccgggtgct gcgcaccgtg
     7441 gggtgcgagc cgggacagct cgagaaggtc ttcgagaggc tccagggaga aaaaggggcg
     7501 ctccagaaga ccctggacaa ggtgcgcccc ctggtgtccc tcccctggga gcgccacgcc
     7561 ctaggggagt ataccctgga tgtggcggtg gtggcggccc ttcccccgga gatggccacc
     7621 cccgccgtga agcgccatgg ggagaccttc ccccggggag tggccctggt gctcctcccc
     7681 ggcactgcag acgacagggt tccctttgtc ctggcccggg gcgccgaggt ggcgatggac
     7741 ctccggaggg cgatcaaaga ccccgaactg ggggcccggg ggggaggggc cccggagtgg
     7801 gtctccggcg tggtccgggg ggcctcggtg gccccgtggc tccgggccct gggagaggcg
     7861 ttatgactcc catccccgac ctccatcgcc tcttccaccc ccggagcgtg gctctcatcg
     7921 gggcctcgga gaagctcgac agcatttcgg gacgccccct gcacctcctc aaggagcatg
     7981 gatttcccgg gagggtctac cccgtgaacc cccgacatcg ggagatcgac ggcaccccgt
     8041 gctttcccga cgtggcctcc ctccccgaga cccccgatgt ggtgctggtg ggggtgcggg
     8101 cggccctggt ccccgccgtc ctggaggcct gcgccgtccg gggagtgccc ttcggcgtga
     8161 tcttcagctc ggggtttgcc gaaagcgacg gcgccgagga ggagcgccgc atggtggaga
     8221 tcgcccgggg cggaggcatg cggcttttgg gccccaactg ccagggcctg gcgaacttcc
     8281 gggaggacat tccgctgacc ttttcttcgt cacttttggg acgcacgccc ctccccggag
     8341 gggtggccta cgtctcccag agcggcgcct tcggctttgc cagcgccgcc caggccatgg
     8401 accggggggt gcgcttccac tccgtggtca ccacgggaaa ccagtgtgac ctggacgccc
     8461 tggacgtggg acgccacctg ctgcgggatc ccgaagtgcg agccctgctt atctacctgg
     8521 agggaatccg ggacggggaa cgctttgtgg ccctggcgga agaggcccgg gagcggggca
     8581 ttcccctggg ggtgctcaaa gttggcaaga gcgcctccag ccgggaggcc gtccagagcc
     8641 acaccgccgc cctggcggga gaccccgagg tgtgggaggc gatctttcgc cagtacggca
     8701 tctttcccct ggaggacgc
//
LOCUS       DAOOER010000072         8596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_8579_length_8596_cov_9.419740, whole genome shotgun sequence.
ACCESSION   DAOOER010000072 DAOOER010000000
VERSION     DAOOER010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 8596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8596
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8579_length_8596_cov_9.419740"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="PL162_08190"
     CDS             <1..1314
                     /locus_tag="PL162_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQF92034.1"
                     /translation="IQGIHRFTFQFFRLDREIDAFLRATSEMSEEVKEGIEGVGHIHT
                     ATDQQYAGSEEISATAQALSILAADLNGAVETVSEKAETGRSRLALMETALKDIEQGM
                     GEVRTHAGRLADRAANIGSVAQVITGISEQTNLLALNASIEAARAGEAGRGFAVVADE
                     VRKLAEESKRAAAQIGESLKELGDSVRETTREIGTMAQKVSASSEHTSQSLEGIAGVL
                     TGIGAVRDASSRVAASAQELSASSQELAASAETVARGTEDLRGRFDRMGGLLKTFEDS
                     SADLERNSQEGVAEASGLVQSLGGLKIITPKDFVTIAEEAIKAHQDWVKRLDGALKGE
                     RWDLETDPTRCRFGIFLSFVPRPDEVPERLWNEGLALHDQLHALGHQAHDALSRGDLP
                     QARRHHDEVGHVSQKLVAALNEMIALCRQGTALEGTPLRALPSSR"
     gene            complement(1738..2109)
                     /locus_tag="PL162_08195"
     CDS             complement(1738..2109)
                     /locus_tag="PL162_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HQF92035.1"
                     /translation="MTRTHTFLCVSCPVGCSLTVEEEEGVLVAVSGESCPRGRRYAET
                     ELKDPRRTFAGTVAVSGGALPVCPVRSRGGIPKGLLADVARAVSQVVVEAPIVIGQVV
                     IADVCGSGIDITASRSLGGRP"
     gene            complement(2113..3369)
                     /locus_tag="PL162_08200"
     CDS             complement(2113..3369)
                     /locus_tag="PL162_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQF92036.1"
                     /translation="MPEVPVDVAIVGAGPAGVAAGIGAREAGAKEVLVLERDWDLGGI
                     LQQCIHPGFGLRTFKEELTGPEFMHRWIERAHGAGVRFSTNTMVFSMDDDGSLWTMNP
                     HRGIEHVVPKAVVLAMGCRERPAGAIKLTGTRGAGIYTAGTAQRFVNMEGLLPGKRVV
                     ILGSGDIGLIMARRMIWEGAEVEGVYEIMPWAGGLRRNVAQCLDDFHIPFHLEHAVLS
                     THGRDRLEGVTVAKIDAQRRPVEGTERYVPCDTLLLAVGLIPENELSRMAGVAIHPGT
                     GGPVVNQFLQTSKPQIFAAGNVVIVYDLADNVSDEGMRAGRNAALYAAGDLPCGGRRF
                     EIKGGKAVRLFSPQWITDGGDATVFFRVVRPFECRCKVFADHGLFSQTHRYARPGEMN
                     EVHLSARDLRSLDPGVTSLTVDVEEV"
     gene            complement(3362..4849)
                     /locus_tag="PL162_08205"
     CDS             complement(3362..4849)
                     /locus_tag="PL162_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQF92037.1"
                     /translation="MTAGNYDVLIVGGGITGATLARELSAFDLRVAVLDKAGDLPSGA
                     SRANSGMLHAGYDDKPGTVKARFCAPGNKRYRELHEMLDFTLRPWGSYVCAFSEADCR
                     HLEKLKDQGARNGVPGLDIVPGDVLRRREPQASPHIVAALHAPTGGVVNNFEAVLAFL
                     DNAQQNGVELFLDTEVRRVLLTEGGGEVLGVETSRGIFLAPVIVNAAGTHSGEIAALV
                     GDESIQIRPTRGEYFITDKAIGSMIKSFFFPCPDERGKGVTVARTADGNLLLGPTSVR
                     QGDKDDTATTPQGLAEVMAGALRLVPSIPQELTITSFAGIRAADRTEDFRIGALARPR
                     GFVHMAGIKSPGLTSAPALALHVVELLREELADRISMKPNPHSIPERTHILRFAALTM
                     EERQDLVRQNPAYAQIVCRCETVTEAQIVEAVRRGARTVASVKMWTRAGSGRCQGGFC
                     CPRVVDILARELHMDPDEVTRHGGPSRLLVGHTKAFWQKREAHHA"
     gene            complement(5149..6663)
                     /gene="glpK"
                     /locus_tag="PL162_08210"
     CDS             complement(5149..6663)
                     /gene="glpK"
                     /locus_tag="PL162_08210"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HQF92038.1"
                     /translation="MTEKKYAVAIDQGTTSTRCMVFDGDGNVVSVDQMEHKQIYPKPG
                     WVEHDPLEIWSRTQDVIRGALTKGEVKSEEIAGIGITNQRETTVVWEKATGKPIYNAI
                     VWQCMRTQSFCEEWGKMPGWKEKTNQETGLVVSPYFSGTKIKWILDNVPGARDRGEKG
                     EILFGNIDTWVIWNLTGGPDGGVHVTDVTNASRTLCMNIRTLQWDDEMLGFFGIPKAM
                     MPAIKPSSYVYGTTRANGPFGGEIPVAGDLGDQQAALFGQACYRVGEAKNTYGTGCFM
                     LLNIGDKPSLSKNGLLTTVAYGLEEGKATYALEGSIAITGAAVQWLRDNLRLFDDAPD
                     SEYFARKVEDSGGVYFVPAFSGLYAPYWDMSARGAIVGLTRYVTKEHIIRATLESICY
                     QTRDVQKAMDADSGVPLTELKVDGGAVKNNLLMQMQADILGTPVVRPVVSETTALGAA
                     YAAGLATGLWKSIEELREHWKADRHFDPVWSDEAREIACKGWEKAITKAKDWSV"
     gene            complement(6686..6868)
                     /locus_tag="PL162_08215"
     CDS             complement(6686..6868)
                     /locus_tag="PL162_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92039.1"
                     /translation="MTRGFSKAREKIDGGEYATIGALCKEGAMVGAVEASIGSPVSRV
                     AAVAREARNLKKFHES"
     gene            complement(6892..7362)
                     /locus_tag="PL162_08220"
     CDS             complement(6892..7362)
                     /locus_tag="PL162_08220"
                     /inference="COORDINATES: protein motif:HMM:NF016216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HQF92040.1"
                     /translation="MISVSAVRQTLAKHPVIPAIREKKNLLRLPKGAPRCVFLLESSL
                     LDIAKTAAEVSRAGHLTFIHVDLVEGLKVDRTPSPPWAFRSLGASRGGVYSMSFAHGA
                     ETPDAGLKPAVFSTGPGVRHTFCHFLTECLATAMLIFGIFVIFSRNTSRSLIRP"
     gene            7863..8498
                     /locus_tag="PL162_08225"
     CDS             7863..8498
                     /locus_tag="PL162_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92041.1"
                     /translation="MWYALALIALLVLAVLFYVRHVRFGILRQVREVEDRLTPLREAL
                     KRGAPPDVALLRALGKDLRTRNPAYLALEEAGKGELFPPEFRTREAFGASDLALWLEH
                     PHALGVPPDELELVETVTVQHDLQGSLEYFVYRFRVAPPARRSSHGWMLGVSGPYSPE
                     ATWPLFSPPGTGSPLQREGRLSGKSLVDRVHHRCLRDGRLDLWIQEALRRR"
BASE COUNT         1520 a         2892 c         2615 g         1569 t
ORIGIN      
        1 atccagggaa tccaccgctt cacctttcag ttcttccgcc tggatcggga gatcgacgcc
       61 ttccttcgcg ccacgtcgga gatgagcgag gaagtcaagg aggggatcga gggcgtggga
      121 cacatccaca cggccacaga ccagcagtac gccgggtcgg aggagatctc cgccacggcc
      181 caagccctct ccatccttgc ggccgatctc aatggcgccg tggagaccgt gtcggaaaag
      241 gccgaaacgg gaagatctcg cctggccctc atggagaccg cgctaaaaga catcgagcaa
      301 ggcatgggag aggtgcgaac ccatgcgggg cgcctggcgg accgcgccgc caatatcgga
      361 tcggtggccc aggtcatcac cgggatctcg gaacagacca acctcttggc gctcaacgcc
      421 tccatcgagg ccgcccgggc gggggaggcg ggccggggtt tcgccgtggt ggccgatgag
      481 gtgcgcaagc tcgccgaaga aagcaagcgc gctgcggctc agatcggcga aagcctgaag
      541 gagctggggg attcggtccg ggagaccacc cgagagatcg gcaccatggc ccaaaaggtt
      601 tcggcctctt cggagcacac ctcccagtct ttggagggca tcgccggagt cctcacgggc
      661 atcggcgccg tgagggatgc ctcgtcccgg gtggccgcca gcgcccagga gctttccgca
      721 tcgtcccagg agctcgccgc cagcgccgag accgtggccc ggggcaccga agacctgcgg
      781 gggcgcttcg accgcatggg ggggctcctc aagaccttcg aagattcctc ggccgacctg
      841 gagcgcaact cccaggaggg agtggccgag gcttcggggc tcgtccagag cctggggggt
      901 cttaagatca tcaccccgaa ggacttcgtg accatcgccg aagaggccat caaggcccat
      961 caagactggg taaaacgcct ggacggcgcc ctcaaggggg aacgttggga tctggagacc
     1021 gatcccaccc ggtgtcgctt cggcattttc ctctccttcg tgccccgccc cgacgaggtg
     1081 ccggaaaggc tctggaacga ggggctcgcc ctgcacgatc agctccacgc cctgggacac
     1141 caggcccacg acgctctctc caggggcgat cttccccagg cccggcggca ccacgacgag
     1201 gtggggcacg tcagccagaa gctagtggcc gccctgaacg agatgatcgc cctctgtcgc
     1261 caggggaccg ccctagaggg aacccccctc cgggcactcc cttcctctcg gtaagatcgc
     1321 ccctccatcg atcatcagat ttttcgccgc cccttatccc gaggagaagg ggcctttctg
     1381 tcctttgtct attcccagcg atttttcccc ttatcccgtc accccggcca ccgacgccag
     1441 gggcatctcc tcccggagta tgtttcgcgc accgaggtgc ggaggtgctc cctacggtct
     1501 ttctcgatgc cctctttgtg cccttcctgg cgggcgtggt tctgcaaggt ggcgatgtcg
     1561 tgcaatccct tttctcggag ctcgtaaaag ccgccgctcg tgctccatct gcatgaagag
     1621 ttctgccgcc gtgcggacct tcgggatcat gggagcctct tgggccatct ggcccatggt
     1681 ttctccctcc gcattgttcc gataaaacag ccccttcggt ccaagggtac gggaggccta
     1741 gggcctcccg ccgagggaac gactcgctgt gatgtcgata ccgcttccac agacgtcggc
     1801 gatgaccacc tggccgatga cgatgggagc ttccaccacg acctgcgaca cggctcgcgc
     1861 cacgtcggcg agcaatccct tggggatccc tcctcggcta cgcacagggc agacgggcaa
     1921 cgcccccccg gacaccgcca cggtccccgc aaaggttcgg cgcggatcct tcagctcggt
     1981 ctcggcatag cgccttcccc gagggcaact ctcgcccgaa acggccacga gaacgccctc
     2041 ttcctcctcc acggtgaggg aacaccccac ggggcaggag acgcagagaa aggtatgggt
     2101 cctcgtcatg aactacacct cctcgacatc gacggtgaga gaagtcacac cggggtcgag
     2161 gctccgcagg tcgcgggcgg aaaggtgcac ctcgttcatc tccccagggc gggcgtagcg
     2221 atgggtttgg gaaaaaaggc cgtggtccgc gaacaccttg caacggcact cgaaggggcg
     2281 cacgacccgg aagaacaccg tggcgtcacc gccgtcggtg atccactggg gcgaaaagag
     2341 acgtaccgcc ttccccccct tgatttcgaa acggcgtccc ccgcaaggga gatctcccgc
     2401 cgcgtagagc gccgcgttcc tccccgcgcg catcccttcg tcgctcacgt tgtccgccaa
     2461 gtcgtagacg atgaccacat tccccgcagc gaagatctgg ggcttcgagg tctgcagaaa
     2521 ctggttcacc acgggccctc cggtcccggg atggatggcg acccccgcca tgcgcgacag
     2581 ttcgttttcg gggatcaacc ccaccgccaa aaggagcgtg tcgcagggga cgtagcgctc
     2641 ggtaccctcc acgggcctcc tttgggcatc gattttcgcc acggtgactc cttccaggcg
     2701 gtcccggccg tgggtggaga gcaccgcgtg ctccaggtga aaggggatgt ggaaatcgtc
     2761 gagacactgg gccacgttgc ggcgcagccc ccccgcccag ggcatgatct cgtagacccc
     2821 ctcgacctcc gccccttccc agatcatgcg gcgcgccatg atgaggccga tgtcgccgga
     2881 gcccaggatg accacgcgct tgccgggaag cagtccttcc atgttgacga agcgttgggc
     2941 ggtgcctgcg gtgtagatcc ccgccccccg cgtgccggta agcttgatgg ccccggcggg
     3001 gcgctcgcgg caccccatgg cgagcaccac ggccttgggg acgacgtgtt cgatgccccg
     3061 gtgagggttc atggtccaaa gagatccgtc gtcgtccatg gaaaaaacca tggtgttcgt
     3121 ggaaaagcgc acccccgccc cgtgggccct ctcgatccaa cgatgcatga actccggacc
     3181 ggtgagttct tccttgaagg tgcgcaggcc aaatccggga tggatgcact gttgcaggat
     3241 acccccgaga tcccagtccc gttcgaggac gaggacctcc ttcgccccgg cctcccgggc
     3301 gccgatcccc gccgcgacgc cggcgggccc cgcgccgacg atggccacgt ctacgggcac
     3361 ctcaggcatg atgggcctcc ctcttctgcc aaaaggcctt cgtgtgtccc accaggaggc
     3421 gcgaagggcc cccgtggcgg gtcacctcgt cggggtccat atggagctcc cgagccagaa
     3481 tgtccaccac ccggggacag cagaatcccc cctggcatcg gcccgatccg gcccgggtcc
     3541 acatcttcac gctggcaacg gtgcgagcgc cgcgccgcac ggcttcgacg atctgggctt
     3601 cggtgacggt ctcgcaacga cagacgatct gggcataggc ggggttctga cgtaccaagt
     3661 cctgacgctc ctccatggtc agtgctgcga accgcaggat gtgcgtcctc tcggggatgg
     3721 aatgcggatt cggcttcatc gagatgcggt cggcgagctc ctcgcggagg agctccacca
     3781 cgtggagggc cagggcgggg gcgctggtga gccccgggga cttgatgccc gccatgtgga
     3841 caaatccccg ggggcgcgcc agggccccga tgcggaaatc ttccgtgcgg tcggcagcgc
     3901 ggatccccgc gaaggaagtg atcgtcaact cctgaggaat cgacggcacg agccgcaacg
     3961 cccccgccat cacttcggca aggccttggg gggtggtggc ggtgtcgtct ttgtcgccct
     4021 gcctcaccga cgtgggcccc aggagcaggt tgccatcggc ggtacgggcc acggtgacgc
     4081 cctttcccct ctcgtcggga caggggaaga aaaagctttt gatcatcgac ccgatggcct
     4141 tatcggtgat gaagtactcc ccgcgcgtcg gccggatctg gatgctctca tctccgacaa
     4201 gggccgcgat ctcccccgag tgggtacctg cggcgttgac gatcaccggg gccagaaaga
     4261 tccctcggga agtctcgacc ccgaggacct cgccgccccc ctcggtcagg aggacgcgcc
     4321 tcacctcggt gtcgagaaag agctccaccc cgttctgttg cgcgttgtcc aaaaaggcga
     4381 gcaccgcctc gaagttgttc accacgcctc ccgtgggggc gtggagcgcg gcgacgatgt
     4441 gcggcgatgc ctggggttcg cgacggcgga ggacgtcccc cggcacgatg tcgagccccg
     4501 gaacaccgtt tctcgccccc tggtccttca gcttttcgag gtgacggcaa tccgcctcgc
     4561 tgaaggcgca cacgtaggaa ccccagggtc gcagggtaaa atccaacatt tcgtggagtt
     4621 cccgatagcg cttgttgccc ggggcgcaaa aacgcgcctt caccgtgccg ggtttgtcgt
     4681 cgtaccccgc atggagcatg ccgctgttgg cccgggatgc ccccgacggc agatcgccgg
     4741 ccttgtcgag cacggcgacg cgcaggtcga aggccgagag ttcccgggcg agggtcgccc
     4801 ccgtgatgcc gccccccacg atgaggacat cgtagtttcc tgctgtcaca cccgtcacct
     4861 ccggtggatc caagggggat ccctgaaacg ctccccaatc cttccggagg cacccatcgt
     4921 tttgcggcgc cgcaccaact tctttcggaa agccgagagg tttcaggatc ccccgcttca
     4981 tcatccatca caaacatcgt tcccgtcgaa accccgagaa cccgccggag caaaccgggc
     5041 gcccaccggt gagggccccc actttcgagc agacgagaaa cgcccaggag gcgaaactcc
     5101 cgttgcggca agaggtttcg ccctccgcct ccggaacact tccgtgggct acacactcca
     5161 atccttcgcc ttggtgatgg ctttttccca gcccttgcag gcaatttccc gggcttcatc
     5221 gctccagacc gggtcgaaat ggcgatcggc cttccagtgc tcacgcaact cttcaatgct
     5281 cttccagagc cctgtggcaa gacccgccgc ataggccgcc ccaagagcag tggtttcgct
     5341 caccacggga cgcaccacgg gcgttcccag gatatccgcc tgcatctgca tgaggagatt
     5401 gttcttcaca gcaccgccat cgaccttcag ctccgtgagg ggcacgccgg agtccgcatc
     5461 catggccttc tggacatccc gagtctggta acagatgctt tcgagggtgg cccggatgat
     5521 gtgttccttg gtgacgtagc gcgtcaggcc cacgatggcc ccccgggcgc tcatgtccca
     5581 gtagggggca tagaggccgg agaaggcggg gacgaagtag accccaccgc tgtcttctac
     5641 cttgcgggcg aagtactcgc tgtccggagc gtcgtcgaag aggcgcaggt tgtcgcgcag
     5701 ccactgcacc gccgcccccg tgatggcgat ggatccttcc agggcatagg tggctttccc
     5761 ctcttcgagg ccataggcca cggtggtgag cagaccgttt ttcgagagcg acggcttgtc
     5821 gccgatgttg agaagcatga agcaccctgt gccgtaggtg tttttcgctt cccccaccct
     5881 gtagcaggcc tgaccaaaga gcgccgcctg ctggtcgccg aggtcccccg ccacgggaat
     5941 ttctccgccg aaggggccgt tcgcccttgt ggtgccatag acatagctcg acggcttgat
     6001 ggcgggcatc atggccttgg ggatgccgaa gaatccgagc atctcgtcat cccactgcaa
     6061 cgtccggatg ttcatgcaaa gcgtccggga agcgttcgtg acgtcggtga cgtgcacgcc
     6121 tccgtcgggg ccgccagtga ggttccagat gacccaggtg tcgatgttcc cgaagaggat
     6181 ctcccccttc tctccccggt cccgggcgcc gggaacgttg tcgagaatcc acttgatttt
     6241 ggttcccgag aaataggggc tcaccacaag ccccgtttcc tggttcgtct tctccttcca
     6301 gcccggcatc ttgccccact cttcgcagaa actctgggtg cgcatacact gccagacgat
     6361 ggcgttgtag atgggcttgc ccgtagcctt ttcccagacc acggtggttt cgcgctggtt
     6421 cgtgatgccg atgccggcga tctcctcgga cttcacctcg cccttcgtga gggcgccacg
     6481 aatgacgtcc tgggtgcgcg accagatttc gagaggatcg tgttcgaccc aacccggctt
     6541 cggatagatc tgcttgtgct ccatctggtc gacgctgacc acatttccat ctccgtcgaa
     6601 caccatacag cgcgtgctgg tcgttccctg gtcaatggcc accgcatact ttttttccgt
     6661 catctccaag tccttctcca caaaatcatg attcgtggaa ttttttgagg tttcgcgcct
     6721 cgcgggccac cgcagccacc cgggagacgg gagatccgat gcttgcttcc accgctccca
     6781 ccatggcccc ctccttgcag agcgccccga tggtggcata ctcgccgcca tcaatctttt
     6841 cccgggcctt cgagaaacct cgggtcatgt tgatgccatg gtccgcgtct cctatggccg
     6901 aatcaagctc cgtgaggtat tccttgaaaa aatgacgaaa atcccaaaaa tcagcatggc
     6961 ggtggcgaga cattccgtga gaaagtggca gaacgtgtgg cgaaccccag ggccggtgga
     7021 aaaaacggca ggcttcaagc ccgcgtcggg ggtttcggcc ccatgggcga aagacatgga
     7081 atagacgccc ccccgcgatg cccccaacga tctgaacgcc catggtggcg atggcgtgcg
     7141 gtccaccttc agaccttcga cgagatcgac atggataaac gtgagatgtc ccgcccggga
     7201 aacctcggca gcggtcttgg cgatgtcgag taaagaactt tcaagaagaa aaacacagcg
     7261 aggggcacct ttggggaggc gcaacaagtt ctttttctcg cggatggcgg gaatgacagg
     7321 gtgtttggca agtgtctgcc gtacggcgga gacacttatc aagggaattc ctcctctgcc
     7381 ggaagtgtgc gtaaaaaaac tccgctcagg ccctacaggg ccttggcctt cgccacgtcg
     7441 cgacggcgac ctggacaaaa acggttgcca gagctcttcc acacgtccca ttttacctca
     7501 tggaacgctc ggaggacccg gtggcgctcc ttcttggttc gggggtcggt gcaacgcaaa
     7561 aaaattcttc ggagctcgcc cccacaaggg ggtcggtgcc ttcaccgccc catgggctct
     7621 tccgccttcg cggcagacaa atccggcgcc acctcaggat tttctccacc ttccaccccg
     7681 ccgcgacggc cccggggacc atgccacgct ccgtctcgaa tcccgattcc cctcactctt
     7741 ttcccgaaaa aggagtgttt cagaaaggtt ttctccgcga agaaacgccc ccccacgttc
     7801 cgcccaaaga ccccatcctc tgctatcatg gcgggagatt ttccctggga ggtgacttca
     7861 tcgtgtggta tgccctggcc ctcatcgccc tgcttgtgtt ggccgtgctt ttctacgttc
     7921 gtcatgtccg cttcggcatc ctgcgccagg tgcgggaggt ggaggaccgc ttgactccgc
     7981 tgcgggaagc tctgaaaaga ggtgctcccc ccgacgtcgc cctcctccgc gccctgggaa
     8041 aagatctgag aacccgcaac cccgcctatc tcgccctgga agaagcggga aagggggagc
     8101 tctttccccc cgagtttcgc acccgggagg cctttggcgc ctcggatctg gcgctgtggc
     8161 tggagcaccc ccacgctctg ggcgttcctc cggacgaact ggaactggtg gaaaccgtca
     8221 ccgtccagca cgacctccag ggctccctgg agtactttgt ctatcggttt cgcgtcgccc
     8281 cccccgcccg gagatcctcc cacgggtgga tgctcggggt ctccggcccc tactcccccg
     8341 aggcgacatg gcccctcttc tccccccccg gcacgggaag ccccctccag cgcgaaggac
     8401 ggctctcggg aaaaagtctg gtggatcggg tccaccaccg atgtcttcgg gacggacggc
     8461 tggatctctg gatccaggaa gccctgcggc gccgctagaa agtaggggag tgccgtggga
     8521 gggagtgcat ggccgggggt gcatagccga agagcgcccc ccgtcccgcc gccccgactc
     8581 cacccttcgc cccggg
//
LOCUS       DAOOER010000073         8433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_8756_length_8433_cov_9.313082, whole genome shotgun sequence.
ACCESSION   DAOOER010000073 DAOOER010000000
VERSION     DAOOER010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 8433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8433
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8756_length_8433_cov_9.313082"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2363)
                     /locus_tag="PL162_08230"
     CDS             complement(<1..2363)
                     /locus_tag="PL162_08230"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007076 - mitotic chromosome condensation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation SMC family protein"
                     /protein_id="HQF92042.1"
                     /translation="MFLERLFLKNFKSFGGSHELRFAPGFTAVVGPNGSGKSNLLDAL
                     RWVLGDGSASRLRITRLGDLAFQGSASLARSTEAEVSLRLREISDGIEGGRGRSCTLG
                     RRFSPEDGGSLFFLDGARVRLQDVDEAKRSWRLEGDRFAFIGQGEVAEVVQQRPRERR
                     VHLESLFGIDIYRRRRDEALEKLSVVGEELRRLALLQGELEGRRREIAPAVRRAEEAR
                     AVMEALQDARRGLYWRRRREAERDQCELAEAQEEQRALFSLLSGWKRGWEGVLGRFNQ
                     AVTPWERESSTLRADFLEKKERREKLHRRAFEEGSALNNLKNLREDLHRRREDGREAR
                     WRCEAALRERSLEMESHREALEVLRSRLAEEEEAWRLVHQGRAEERALRDEATAALAA
                     VREEAPRVAGRLTSLGRRWRAVVEEERDGVKAQERLGREVEEGRRRLSRSEQDAADAV
                     RRAGDAYALCQSMGGDLQRERRELATLRHRGEELKEQLQGNIYPRPVQHVLSAARLGR
                     LAARPVPALETFQCPATLWAPLDAYLGGRRFWLFVQDFAEAGRCIDQLKKARAGRATF
                     LPMEQAKPRHPDRRVTLPPRGVVGWALDLVTPDPAWEAGVAHLLGDLLVVETYAVGVE
                     LVRREPRIPVVTLEGDVFLPGGTVTGGASGREKGPMELRHQLGMVEEEVRGRERRLTE
                     LEWGLKEAEGRERAAAKLREESALAVGREEKALAGVRSAKERVDQRLEALGEEREELL
                     RVLGEEGRRLAAARQRERELCDALGRLPSVPEERAWESRVVALRGEVA"
     gene            complement(2338..3834)
                     /locus_tag="PL162_08235"
     CDS             complement(2338..3834)
                     /locus_tag="PL162_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HQF92043.1"
                     /translation="MTLGRKIVANLRDPEEVRVAILEDGRLAELLVEHMWERQRAGEI
                     YKARVDSVLGGMQSAFLNLGDGRNAFLHLADARGMEVRPNTEMLVQVVKAARKGKGAR
                     VSPRLALPGRHLVLVPGGHDIGVSRRIADDRERRRLRKMARDLRPEGFGLIVRTVSEG
                     VGEGELGTDVEELLALWREIEHQSRVQPAPCLLYRDLGLLGKVLRDELNDAVDEIVLD
                     SSEDEERIRGYVTRCFPSSSPEVSVYSGVTPLFDYLGIEREISAALDRKVWLPSGAYL
                     VVEQTEALTVIDVNTGKFVGDVDLRHTVLDTNLEAAGEIARQLRLRGIGGIVVVDFID
                     MDHAQDRKALLDRLEEVFRSDRCRAKIFGVTELGLVEITRKRARVDLRTALTRGCPEC
                     GGAGWVMKEESLALVLKRFLRKVCHSGRAEAYLLEVHPALGTYVMENCLGLWEEEFGR
                     RFFLVKAPEFAWGKFRLDAQGTLGQMEHRLQVWKTREPFSGVLGTAIP"
     gene            complement(3831..4421)
                     /locus_tag="PL162_08240"
     CDS             complement(3831..4421)
                     /locus_tag="PL162_08240"
                     /inference="COORDINATES: protein motif:HMM:NF021610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HQF92044.1"
                     /translation="MRVRFFFAKRGRSAFFPHVELPPIFVRSARRAGLEFEYSEGCVP
                     HPRLSLGPTLPLGIVALEEPAEGWFSSFPADAAALWGSKFPPGLEMIEAFPVEGPPLA
                     AVCPRAHYLLRPRRAEDLSSLEEALAVSGGGDVRWHRTSEGLSVTMGAERSMGGVLRA
                     FGEISPGRGWRDVFVVRLRVGPEEGDGVRSLREVRR"
     gene            complement(4427..6259)
                     /locus_tag="PL162_08245"
     CDS             complement(4427..6259)
                     /locus_tag="PL162_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HQF92045.1"
                     /translation="MVDFPEERDELWPLLASVKRGARYVGGEWGTLPLKPDAPGLVRL
                     CLAFPDVYEVGMSYLGFQILYRLVKSLSYADVERAYCPWPDMEQALRSKGMGLGSLES
                     GKVLGDFDVVGFTLQYELTYTNLLTMLDLGKIPLHREDRGDKDPLVFAGGPGALVPEP
                     LASFVDVFCVGDGEELLPQALAILSELRGSPRELRLEALASLPGIYVPAWALPGGPRG
                     FVGSRGMVLPVERRWVADLDRTFFPEHMMIPSLGVVHDRVPVEVFRGCTRGCRFCQAG
                     MVTRPVRERSPETIGRLALSLLEDSGCRELGLLSLATCDYRRLDELLALLVPELERRR
                     ASLSLPSLRIDGFSVDLATRLEGVRRGSLTFAPEAGTERLRRVINKGLSDGEILSTVE
                     AAFRHGWERIKFYFMMGLPTESDMDLEGLEALVRECARIGRATARGAQIAVSVAGFVP
                     KAHTPFQWEEQSLPEDLRDRGRRIKSGLRKERSVKVAYHEPAQTFLEGVFARGGRECG
                     AVLERAWRGGARFDGWDECFRFDLWEEAFRREEIDPRALVSRPRGRDEVFPWDHIDAG
                     VSREFLLRERDRAYDELLSPDCRTGECQGCGLGDRCAPSGIGGV"
     gene            complement(6389..7504)
                     /locus_tag="PL162_08250"
     CDS             complement(6389..7504)
                     /locus_tag="PL162_08250"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HQF92046.1"
                     /translation="MGNDNITTFSERLRHLDRWALASGVALFLLGLLSIFSATYGRSE
                     EFVLRQIFSGGLAAVGYGVAIALGHDRLLRWAYAIFGVTVSILALVLTLGTMVKGSQS
                     WFALGPLRLQPSEMGKVALALALGKHFCRYPPLTFKAFVYDLLLPLAGGVLVLLQPDL
                     GSALVYGVMTLGALVVAGAPKRFLAGLFGAGLLALPVGWSLLKDYQKLRLLVFLDPQI
                     DPLGAGYNVIQSRIAVGAGGLWGKGFLEGTQSKLRFLPEPHTDFIFSVYAEEFGFVGG
                     VLLLVLFGILLWRIFGAGMRSKDLRCKVLAGALGSWIWFQVVECVAMSMGYAPVTGLP
                     MPLFSYGGSALLAVGVGLGIVQSIHLDTLRPYQESEK"
     gene            complement(7494..7778)
                     /gene="minE"
                     /locus_tag="PL162_08255"
     CDS             complement(7494..7778)
                     /gene="minE"
                     /locus_tag="PL162_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HQF92047.1"
                     /translation="MSLFSRFFGQQESRSVAKDRLQLVLIHDRSDISPEVMENLRRDL
                     IDVISKYMDIDESHIELQLERENSSVALVANIPVRTVRRRRGSERRAHGE"
     gene            complement(7807..>8433)
                     /gene="minD"
                     /locus_tag="PL162_08260"
     CDS             complement(7807..>8433)
                     /gene="minD"
                     /locus_tag="PL162_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HQF92048.1"
                     /translation="YTLVDVVEGGCRLNQALVRDKRLKNLYLLPAAQTRTKDAVDSDQ
                     MAALCDELRETFDFVLVDSPAGIEGGFRNAAQAADEALVVTTPEVSAVRDADRIIGML
                     ESMNKSPIRLVVNRLRPDLVKRGEMLSVQDVLDILAVDLVGVVPNDDSVIDAANKGEP
                     LTLSSTTPASQAFINIALRLLGEDVPFMDLSQEESAGFGKRLRKFLGF"
BASE COUNT         1626 a         3117 c         2321 g         1369 t
ORIGIN      
        1 gccacctctc ctcgcagggc caccacccgg ctctcccaag cccgctcttc gggaaccgaa
       61 ggcagccggc ccagggcatc acagagctct cgctctctct ggcgagccgc cgccaagcgt
      121 cgcccttctt ctcccaggac ccggaggagc tcttctcgtt cctctccaag agcttcaagc
      181 ctctgatcca cccgctcttt cgcggatcgc acccctgcca aggccttttc ttcccgcccc
      241 accgccaggg ccgactcctc ccggagcttg gctgcggccc gctctcgccc ttcggcctcc
      301 ttcaggcccc actccagctc cgtcaggcgt cgttcgcgcc cccgcacctc ttcttcgacc
      361 atgcccaact gatggcggag ctccatgggg cccttctctc ggccggaggc ccctcccgtc
      421 accgtccccc cggggaggaa gacgtctcct tccagcgtga ccacgggaat gcgaggctct
      481 cggcggacca actccacccc cacggcgtag gtctccacca ccagtaggtc tcccagaagg
      541 tgggccaccc ccgcctccca ggcaggatcg ggggtgacca aatccagggc ccatcccacc
      601 actccccggg gtggcaaggt cacccggcgg tcgggatggc gaggcttggc ctgctccatg
      661 gggagaaagg tggcccgccc cgccctcgcc ttcttgagtt ggtcgatgca ccgtccggct
      721 tcggcaaagt cctggacaaa gagccaaaag cgccgtccgc caagataggc atccagaggg
      781 gcccacaggg tcgcggggca ctggaaggtc tccagagcgg gtacggggcg ggcggcaagg
      841 cgtcccagac gggcagcgga aaggacgtgc tgcaccggcc ggggatagat gttcccctgc
      901 aactgttcct tcaattcctc cccccggtgg cggagggtcg ccagctcccg gcgctctcgc
      961 tggaggtctc ctcccatgct ctgacagagc gcataggcgt cccccgcacg gcgcaccgca
     1021 tcggcggcat cctgctcgct tcgggaaaga cgccggcgcc cctcttccac ctcgcggccc
     1081 aggcgttcct gggccttcac gccatcccgc tcttcttcca ccacggcacg ccaccgccgc
     1141 cccaaagagg tcaagcgccc cgccacccgg ggagcctctt cccgcaccgc cgccagggcc
     1201 gccgtggcct cgtcccgcag cgcccgctcc tcggccctcc cttggtggac caggcgccag
     1261 gcctcctctt cttccgcgag gcggcttcta agcacctcca gggcctcccg atgactttcc
     1321 atctccaaag agcgctccct caacgccgcc tcgcatcgcc agcgggcttc cctaccatct
     1381 tcacggcgtc gatggaggtc ctctcgaaga tttttaagat tattcagagc gcttccctct
     1441 tcgaaggccc ggcgatggag cttttcccgc cgttcttttt tctcgaggaa atcggccctc
     1501 agagtcgaag actcgcgttc ccagggcgtc accgcctggt tgaagcgccc caacacccct
     1561 tcccatcccc gtttccaccc cgaaagcagg gaaaaaagtg cccgctgctc ctcttgggct
     1621 tcggccaact cgcactgatc cctttcagcc tcccgccgcc gacgccagta gagccctctt
     1681 cgagcgtcct gaagggcctc catcaccgcc cgggcctctt cggctcgccg caccgccggg
     1741 gcgatctccc gtcgacgccc ttccagctct ccctggagca gagccaaacg ccgcagctcc
     1801 tcgcccacca ccgacagctt ttccaaggcc tcatcccgcc gccggcgata gatgtcaatg
     1861 ccgaagaggg actccagatg cacccggcgc tcccgcgggc gctgctgcac cacctccgcc
     1921 acttctccct ggccgatgaa ggcaaagcga tctccctcca atctccacga tcgcttggcc
     1981 tcatcgacgt cttgcaggcg cacccgggca ccgtccagga aaaagaggct ccccccatct
     2041 tcgggggaaa agcgcctccc cagggtgcaa gagcgccccc ggcccccttc gatcccgtcg
     2101 gagatctcgc gcagcctcag ggaaacttcg gcctccgtac tccgggctaa agaggcgctc
     2161 ccctggaagg ccaagtcccc caggcgggtg atcctcagcc tggaggcgct cccatccccc
     2221 aggacccacc ggagggcgtc caggaggttg ctcttgccgc tgccattggg acccaccacg
     2281 gcggtaaatc ccggggcgaa gcgaagctcg tgggagcccc cgaagctttt gaagttctta
     2341 aggaatagcc gttccaagaa caccggaaaa aggctccctg gtcttccaga cttgaaggcg
     2401 atgctccatc tgccccaggg tcccctgggc gtccaggcgg aactttcccc aggcaaactc
     2461 cggagccttc accaggaaaa accggcgccc gaactcctct tcccagagac ccaggcagtt
     2521 ttccatgacg taggtcccca gggcgggatg gacttctaaa agataggcct cggcccgccc
     2581 ggagtggcac acctttcgca aaaagcgctt gagcaccaaa gccaggctct cttccttcat
     2641 cacccacccc gccccgccgc actcgggaca cccccgggtc agcgccgtgc gcaggtccac
     2701 ccgggcgcgc ttccgggtga tctccaccag gcccagctcc gtcacgccga agatcttggc
     2761 tcgacatcgg tccgagcgaa agacctcttc cagccggtcc aacaacgcct tgcggtcttg
     2821 ggcgtggtcc atatcgatga agtccaccac cacgatgccc ccgatgcccc ggagcctcag
     2881 ttggcgggca atctcccccg ccgcctcgag gttcgtgtcc agcaccgtgt ggcgcaggtc
     2941 cacgtccccc acgaacttcc ccgtgttgac atcgatcacc gtgagggctt ccgtctgctc
     3001 caccaccagg taagcccccg aagggagcca gaccttccga tccaacgcgg cggagatctc
     3061 tcgctcgatg ccgaggtaat cgaaaagagg ggtgacgccg gagtacaccg acacctcggg
     3121 ggaggaggag gggaaacacc gggtcacgta gccccggatg cgctcctcgt cttcggaaga
     3181 gtccagcacg atctcgtcca ccgcatcgtt gagctcgtcc cgcaacacct ttcccaaaag
     3241 tcccaggtct cgatagagga ggcacggagc gggctgcacc cggctctggt gctcgatttc
     3301 cctccagagg gccaggagct cctccacgtc ggtccccagc tctccctctc ccactccctc
     3361 ggagaccgtg cggacgatga gcccaaaccc ctcgggacgg aggtcccggg ccatcttgcg
     3421 taggcgtcgc cgctcccgat cgtcggcgat gcgccgggac accccgatgt cgtgccctcc
     3481 gggcaccagc accaagtgcc tgccgggaag ggccaatcgc ggggagaccc gggccccttt
     3541 ccccttacgg gcggccttca ccacctgaac caacatctcc gtgttggggc gcacctccat
     3601 cccccgggca tcggccaggt gaagaaaggc gtttcgccca tctcccagat tcaaaaaggc
     3661 cgactgcatg cccccgagga cgctatccac ccgggccttg tatatttccc ccgcccgctg
     3721 gcgctcccac atgtgctcca ccaggagttc cgccaggcgt ccatcttcca gaatggccac
     3781 ccgaacttcc tcaggatccc ggaggttggc cacaatcttt cgtcccagag tcaccgtcgc
     3841 acctcccgaa gggaccgaac cccgtcccct tcctcgggcc ccacccggag gcgcaccacg
     3901 aagacgtctc gccatcccct tccgggagag atctcaccga aggcccgaag cacccctccc
     3961 atgctccgct ccgcccccat cgtcaccgac agcccctccg aggtccgatg ccaccggaca
     4021 tcgccccccc ccgacaccgc cagggcttct tccagggagg aaaggtcttc ggccctacgg
     4081 gggcgcagca aatagtgggc ccgggggcag accgccgcca gcgggggacc ctccaccgga
     4141 aaggcttcga tcatctcaag gcccggcgga aacttgcttc cccagagggc tgcggcatcg
     4201 gcgggaaagg aggaaaacca cccttcagcg ggctcttcca aagccacgat ccccaggggt
     4261 agcgtgggac ccaggctcaa ccgagggtga gggacacatc cctcggaata ctcgaactcc
     4321 agccccgccc tccgggcgct ccgcacgaag atcgggggca gttccacgtg gggaaaaaag
     4381 gccgaccgcc ccctcttggc aaagaaaaaa cgcacccgca ccgccatcac actcccccga
     4441 ttcccgatgg agcgcaccga tcccccaggc cacacccctg acattccccc gtacggcagt
     4501 ccggggagag gagctcatcg taggcccggt cccgctctcg aagcaaaaac tcccggctca
     4561 ctccggcatc gatgtgatcc caagggaaaa cctcgtcccg tccccgggga cgggagacca
     4621 gggcccgggg atcgatttct tcccgccgga acgcctcctc ccaaaggtcg aaacgaaagc
     4681 attcgtccca gccatcgaaa cgggcccccc cccgccaggc tcgctccagc acggccccgc
     4741 actcccgacc tccccgggcg aagaccccct ccagaaaggt ctgggcgggt tcgtggtagg
     4801 ccactttcac cgaccgctcc ttccggagac cacttttaat gcgccttccc cgatcccgga
     4861 gatcttccgg aaggctctgt tcctcccact ggaagggcgt gtgagccttg ggtacgaaac
     4921 ccgccacgga aacggcgatc tgagcccccc gggcggtggc acgccctatg cgggcgcact
     4981 cccgcaccag ggcctccagc ccctcaagat ccatgtcgct ttctgtgggg agccccatca
     5041 tgaaataaaa ctttattctt tcccagccgt ggcgaaaggc cgcctccacg gtactcagaa
     5101 tctccccgtc cgaaagcccc ttgttgatca cccggcgcag gcgctccgtc cccgcctcgg
     5161 gggcaaaagt caggcttccc cggcggaccc cctccagccg cgtcgccaga tccaccgaaa
     5221 agccgtcgat cctcaggctc ggcaagctca gggaagctcg gcgtcgttcc agctccggca
     5281 ccaaaagggc caagagctca tccaggcggc ggtagtcgca ggtggccaat gataaaagcc
     5341 ccagctcccg acaccccgag tcttccaaaa gggatagggc caggcggcca atggtctccg
     5401 gagagcgctc ccgcaccggt cgggtcacca tccccgcctg acagaaacgg cacccccggg
     5461 tacacccccg aaagacctcc acgggcactc ggtcgtgcac cacccccaac gaagggatca
     5521 tcatgtgctc cgggaaaaag gtccggtcca gatcggccac ccatcggcgc tccaccggta
     5581 acaccatgcc ccgagacccc acgaagcccc ggggtccccc ggggagggcc caggcgggaa
     5641 cgtagatccc cggcagggaa gccagggcct ccaggcggag ctcccgaggg ctcccccgga
     5701 gctccgaaag gatggccagg gcctggggca ggagctcttc cccatccccc acacagaaga
     5761 catctacgaa ggatgccaag ggctcgggga ccagggctcc cggccccccg gcaaagacca
     5821 gcgggtcttt atcgccccgg tcctcccggt gcaacgggat ctttcccagg tctagcatgg
     5881 taaggaggtt cgtgtaggta agctcatatt gcagggtaaa gcccaccacg tcgaaatccc
     5941 ccaagacctt tccgctctcc agggagccga ggcccatccc tttggaccgg agggcttgct
     6001 ccatgtcggg ccaggggcag taggcccttt caacgtcggc gtaggaaagg gatttcacca
     6061 ggcggtagag gatctggaac ccaagatagc tcatccccac ctcataaaca tcggggaagg
     6121 ccaaacaaag cctcaccagg ccaggggcat cgggcttcag gggcaaagtc ccccattctc
     6181 cccccacata acgggctcct cgtttcacgc tggccaaaag gggccacaat tcatcccgtt
     6241 cttcaggaaa atccaccatg tcaccgcctc gctccgccgg aatcttccgg cccatcgccg
     6301 accccgcagt cccgccatgg gccgtgaagg cgccttttgc cgacgtcctc gcccttcgga
     6361 atcctggggg gcatttttat taaaaaaatc acttttcgct ttcctggtag gggcgtaagg
     6421 tgtccagatg gatgctctgg acaatgccga gccccacccc cacggccagc aacgcgctgc
     6481 cgccgtagct gaaaaggggc atggggagcc ccgtcaccgg agcgtatccc atgctcatgg
     6541 ccacgcactc caccacctga aaccagatcc aagaccccaa agctcccgcc aggaccttgc
     6601 agcgaaggtc tttggaccgc atccccgccc cgaagatcct ccagagcagg atgccaaaaa
     6661 gcaccaacag aagcacgccg ccgacgaagc caaattcttc ggcatagacg ctgaagataa
     6721 aatctgtatg cggctcgggg agaaagcgca acttgctctg ggttccctcc aggaacccct
     6781 tcccccaaag cccccctgcc cccacggcga tgcgagattg gatcacgttg tatcccgcac
     6841 cgaggggatc aatctggggg tcgagaaaga ccagcaggcg aagcttttgg tagtccttga
     6901 gcagggacca gccgacgggg agggccagca ggcccgctcc aaagagtccc gccaaaaatc
     6961 tcttgggagc ccccgccacg acgagggccc ccagcgtcat gaccccatat accagggcgc
     7021 tccccaggtc gggctgaagc aacaccaaca ctccccccgc caagggcagg aggaggtcgt
     7081 agacaaaggc cttaaaagta agaggggggt agcgacaaaa gtgtttcccc agagctaacg
     7141 ccagggccac cttccccatc tccgagggct ggaggcgcaa ggggcccagg gcaaaccaac
     7201 tctgggaacc cttaaccatg gtgcccaggg tcaagaccaa ggccaggatc gacaccgtga
     7261 cgccgaagat cgcgtaggcc caccgcagca ggcgatcgtg ccccagagcg atggccactc
     7321 cataccccac cgccgccagt cctccggaaa agatctgtcg gaggacgaac tcctccgatc
     7381 tcccgtaggt ggcgctaaaa atcgacagga gccccaacag gaaaagggcc accccgctcg
     7441 ccagggccca gcgatcgagg tgacgcagcc gctcagaaaa agtagttata ttatcattcc
     7501 ccatgggctc ggcgctcgga tccccggcgc cgcctcaccg tccgcacggg gatgttggcc
     7561 accagagcca ccgaactgtt ctcccgctcc aattggagct cgatgtggct ctcgtcgatg
     7621 tccatgtact tgctgatgac gtcgatcaag tctcggcgaa gattttccat cacctcgggg
     7681 gagatgtcgc tgcggtcgtg gatcagcacc agttgcagtc gatccttggc cacgctccga
     7741 gattcttgct gaccgaaaaa acgcgagaaa agactcatgt cccatcccct cctttctcaa
     7801 aacaagttaa aatcccaaaa actttcgaag ccgtttgcca aatcccgcgc tctcttcctg
     7861 gctgagatcc atgaagggta cgtcttcccc caaaagacgc aaggctatgt tgataaaggc
     7921 ctgagaggcc ggagtcgtgc tggatagcgt cagtggttcc cccttgtttg ccgcatctat
     7981 cacgctgtcg tcgttgggga cgactcccac caagtccacc gccaggatgt ccaacacatc
     8041 ctggacgctg agcatctccc ccctttttac caaatcgggg cgcagccggt tgacgaccag
     8101 gcgaatgggg gatttgttca tggattcaag catcccgatg atgcgatccg cgtcccgcac
     8161 cgccgaaacc tccggcgtgg tgaccaccag agcctcgtcc gctgcctgag ccgcgttgcg
     8221 aaaccctccc tcgatgcccg cagggctgtc caccaggacg aagtcgaagg tctctcggag
     8281 ttcgtcgcag agggccgcca tctggtcgga gtccaccgca tccttggtac gcgtctgggc
     8341 cgctggcagg aggtagaggt ttttcaggcg cttgtcccgc accaacgcct gattcaagcg
     8401 acaccccccc tccaccacat ccaccaaggt gta
//
LOCUS       DAOOER010000074         8398 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_8798_length_8398_cov_7.700228, whole genome shotgun sequence.
ACCESSION   DAOOER010000074 DAOOER010000000
VERSION     DAOOER010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 8398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8398
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8798_length_8398_cov_7.700228"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1216
                     /locus_tag="PL162_08265"
     CDS             <1..1216
                     /locus_tag="PL162_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92049.1"
                     /translation="TPLYHFGMTAQLKAIMERTLPLADPHIVRQGSTYTHDLREGLSP
                     SKAVLLSNCGFPEIHHFDGLREQFRCLARAEGREDGAPADAVILRPGGEILREEALRP
                     MITWYFDALRQAGGEFVAQGKISPQTQEILSRDLLPPEDFVREANRHFDEILASVSGA
                     PEGKERAPERGETSVVRRTPRPASKAPEAGTLRIPRGPVALTGYQWLGVSFVPWAFFW
                     LLAGLGEGEMVFLRTLIPLLLSGALLFYRRRFIDVTLFDLGSVAFFAGELFLSQVHPA
                     FFSAFGDTWSYLALGALWMGSLITGTPLSAPYSRWHVAPEVARHPLFLRTNAWISLFW
                     GGVFLLGAALDTAFAHFDVRGLWSTVWTLVLLVPATWFTQWFIEWYPGRARKKAMLGP
                     QAGGRGGSEARR"
     gene            1213..2172
                     /gene="pfkA"
                     /locus_tag="PL162_08270"
     CDS             1213..2172
                     /gene="pfkA"
                     /locus_tag="PL162_08270"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HQF92050.1"
                     /translation="MRRLAVLTSGGDAPGMNAAIRAVTRAAIAQGVEVVGIRRGYEGL
                     LEGDIIPMSISSVGGIIHRGGTILRTARCERFLRPEGVNEGLDQLRAFDIDALVVVGG
                     DGSFRGAWELDQRGFPVAGVPGTIDNDVAGTDATIGFDTAANTALEAVVRLRDTASSH
                     DRLFIVEVMGRGSGYLALYVGVSGGAELVVVPEVPFDLERTCERLHYGRKRGKTHSLI
                     VLAEGVMPAHELAAKLQDTGGYDARITVIGHIQRGGGPTAADAVLASRMGAHAVEALL
                     AGERGIMVGSVDNVMVTSPLPTAWESKKALPQTLLRLVDTLSI"
     gene            2181..3146
                     /locus_tag="PL162_08275"
     CDS             2181..3146
                     /locus_tag="PL162_08275"
                     /inference="COORDINATES: protein motif:HMM:NF025694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4392 domain-containing protein"
                     /protein_id="HQF92051.1"
                     /translation="MREKDGIWGDDERSLPPEAREALPGEGPEGRALRDERVWDELGE
                     AFREVVASDAGGRGVASLYDPRAWARGVGLLARSRHPVLVTGFVVPPWGVAETDGPPG
                     VAVLARALKGRGVAPRVVTDDRCLPVVRAACEALGGARLQGSHAGDGVLEPDSDLLVF
                     VERLGRAEDGGYYNMRGEEIGAWTAPLDDAAALARARGIPVVAVGDGGNEAGMGNYAR
                     ELGALVPAFSRCLSVVPADATLVVDVSNWGAYILAAGLSLEEGRWLGHDEEEEEALGE
                     AMVAAGAVDGATKRPEATVDGLPPERHRGVVRRIRELWERPRRSF"
     gene            complement(3219..3713)
                     /locus_tag="PL162_08280"
     CDS             complement(3219..3713)
                     /locus_tag="PL162_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HQF92052.1"
                     /translation="MTSPNVARTVALGAVCTALVTVATMLSVPVPGFRLYFNLGEGVI
                     YTVALLLGRRFGAAAGGLGAALADLLLGYALWAPLTLIIKGAEGFVVGTLAPKGRLKA
                     LLGGAVVMITGYTLSAGVLYGWAAAPVELGTDILQTGAGAAFALILVPVLARRVPFLQ
                     KGSL"
     gene            4157..4813
                     /gene="fsa"
                     /locus_tag="PL162_08285"
     CDS             4157..4813
                     /gene="fsa"
                     /locus_tag="PL162_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HQF92053.1"
                     /translation="MRFFLDTANLDEIGQAMAWGVVAGVTTNPTLVSKEGNLDFFTRV
                     REIAEIVKGPVSAEVISLDTPGMVREARELAALSPHVVVKIPMTPEGMGAVRILSEEG
                     INTNVTLVFSSPQAILAAAAGATYVSPFVGRLDDVGESGTELLREIAAIYDVQGIETE
                     IIAASIRNPRHVVDAAVAGCHIATVPFKVLQQLFDHPLTKSGVKRFLEDWETYRRHHA
                     "
     gene            4806..6128
                     /gene="rho"
                     /locus_tag="PL162_08290"
     CDS             4806..6128
                     /gene="rho"
                     /locus_tag="PL162_08290"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HQF92054.1"
                     /translation="MPEQEAVPAQLENGNAEGGEARPRPRHSFSALAGLNVPELRRLA
                     RELEVSGASSLRKDDLIVAVLKAQSERMGFRFGGGTLEILAEGYGFLRPKGLLPSDHD
                     IYVSASQVRRFGLRNGDVVWGVVRAPKEAEHYEALLRVELVNFTDPEIARKRPQFEHL
                     VPIFPNERLRLETEQKIISTRLVDLFAPIGKGQRALLVSPPKAGKTTLLKQLAHAITD
                     NHQDVILMVLLIDERPEEVTDFSRAVDGEVIASTFDRPVEEHMRVANLTLEKAKRLVE
                     AGKDVVLLLDSITRMARASNLVVPPSGRTLSGGMDPAGLYFPKRFFGAARNIENGGSL
                     TIIATALIDTGSRMDDVIYEEFKGTGNMEVHLSRKLSEQRIFPAIDITRSGTRREELL
                     LDEEELPRVWTLRRRLSSVDEAEALSLIIDKVRSTASNRDFLATIKGA"
     gene            6305..7666
                     /locus_tag="PL162_08295"
     CDS             6305..7666
                     /locus_tag="PL162_08295"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HQF92055.1"
                     /translation="MFFLVGMTVLLSATVILAGKRAEERGFAPFSVAPFPDATQGDGY
                     ALAEMPQDDAEGMPLPPEGGATVALMGGLLSGDEGIEIGPFQAPDFFLSDDVILEEKG
                     PSDEEGHSLLGPRKALDDRPVWEQHEVAPGDTLSSIAKKYGVSQECIAQANPTMKNRD
                     RLSLRQVLLIPRSSADIPAVLEEHQRRQDEIVAQKKSAKPVVVSSYAVVGGDSLWSIA
                     GKFNLDINTLLGCNELKDPDRLPPGATLRIPNQDGIFYVFRKGDTLKKISDRFGIYEE
                     AIWAANSMDRGSVLEVGKTTLFLPAAKPITVVQQAEGGRRDSISSPTKKAGGFVWPLQ
                     GRINSSFGWRRDPFSRRKDFHTGLDIKGTYGRPIGAAKAGTVVYAGWMGGYGRTVVIN
                     HGGGYTTLYGHCSKLLVRQGQKVEARKIIARCGSTGRSTGTHLHFEVRRNNVPINPRS
                     VLR"
     gene            7755..>8398
                     /locus_tag="PL162_08300"
     CDS             7755..>8398
                     /locus_tag="PL162_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92056.1"
                     /translation="MEGPRWAHQRLHDRWSTLLLRFGFSQGVTPCLGALRHTGGASGA
                     MVCAAPEVAEAMPQEERSTPSEGGALSSGWRLVASRGVATPHQDLERLLPALGRLEGA
                     RFPVRLSLAKGPWVLLPVRDGETFLGGLALWTLWGRSIAVGDFAYLQELAALLGKAWG
                     REHHMAVLHRALRLTAAANLVGGLLENEGITPQALFSSALEQVRRVIPFDRCSI"
BASE COUNT         1448 a         2584 c         2929 g         1437 t
ORIGIN      
        1 cacgcccttg taccacttcg gcatgacggc ccagctgaag gccatcatgg agcggaccct
       61 tcccctggcc gatccccaca tcgtgcgtca gggttccacc tacacccacg acctgcggga
      121 gggcctttcc ccatcaaagg ccgttctcct gtccaactgc ggttttccgg agatccatca
      181 ctttgacgga ttgcgggagc aattccgctg tctggcccgc gccgaggggc gggaggacgg
      241 cgctccggcg gacgcggtga tcctgcgccc cgggggcgaa attcttaggg aagaagccct
      301 tcgacccatg ataacgtggt actttgacgc cctgcgtcag gcgggagggg agttcgtggc
      361 ccaggggaaa atttctcccc agacccagga gatcctatcc cgggatctcc tgcccccgga
      421 ggacttcgtg cgagaggcaa atcgccattt cgacgagatc ctggcgtcgg tctccggagc
      481 tcccgagggg aaagagaggg ccccggagag gggcgaaact tcggtggtcc ggcggacccc
      541 tcgccccgcg tcgaaggccc cggaggccgg aacccttcgc attccccggg ggcccgtggc
      601 gctcacgggc tatcagtggc tcggagtgtc ttttgttccc tgggccttct tttggctttt
      661 ggcggggctg ggagaggggg agatggtctt tctgcgaacc ctgatccccc tgttgctctc
      721 cggggccctg ctcttttacc ggcggcgttt catcgacgtt accctctttg acctgggaag
      781 cgtggccttt ttcgccgggg agttgttcct atcccaggtc catccggcct ttttcagcgc
      841 cttcggggat acctggagct atctggccct gggggccctt tggatgggat ccctcatcac
      901 gggcactccc ctttcggcgc cctactcccg gtggcacgtg gccccggagg tggcccgcca
      961 tcccctcttc ctacgtacca atgcctggat ctccctcttc tggggagggg tctttttgct
     1021 cggggccgcc ctggacacgg ccttcgccca tttcgacgtt cgggggcttt ggagtacggt
     1081 gtggaccctg gtgctcctgg tccccgccac gtggttcacc caatggttca tcgaatggta
     1141 ccccgggcgg gcccgtaaaa aggcgatgtt gggccctcag gcgggggggc gtggcggatc
     1201 ggaggcgagg cgatgaggcg tctggcagtg ctgacgagcg ggggagacgc gccggggatg
     1261 aatgccgcca tccgggcggt gacccgggcg gcgatcgccc aaggggtgga ggtggtgggc
     1321 attcgccgag gatacgaggg gctcctggag ggagacatca tccccatgtc catcagctcg
     1381 gtggggggca tcatccaccg gggagggacc atcctgcgta cggcgcggtg tgagaggttc
     1441 ctgcgccccg agggggttaa cgagggattg gatcaactgc gggccttcga catcgacgcc
     1501 ctggtggtcg tcggagggga tggatccttt cgaggagcct gggagctgga tcagcggggc
     1561 tttcccgtgg ctggagttcc cggtaccata gacaacgacg tggcgggcac cgacgccacc
     1621 atcgggttcg ataccgccgc gaacaccgcc ttggaagcgg tggtgcgtct gcgggacacg
     1681 gcgtccagcc acgatcgtct cttcatcgtg gaggtcatgg ggcggggatc gggctatctg
     1741 gcgctctacg tgggggtatc ggggggggcg gagctcgtgg tggtccccga agttcccttc
     1801 gacctggaaa ggacctgcga gaggctgcac tacggccgaa aacgaggaaa gacccattca
     1861 ctcatcgttt tggccgaagg ggtcatgccc gcccacgagc tggcggcgaa gctccaggac
     1921 accggggggt atgatgcccg catcaccgtg atagggcaca tccagcgggg cggaggcccc
     1981 acggcggcgg acgcggtgct ggcgtcccgc atgggggccc atgcggtgga ggctctactg
     2041 gcgggggagc gagggatcat ggtggggagc gtcgacaacg tcatggtgac gtcgcccctt
     2101 cccacggctt gggaaagcaa gaaagccctt ccccaaaccc tcctgaggct ggtggacacg
     2161 ctgagcattt gaggtgagcc gtgagagaaa aagacgggat atggggagac gacgaaaggt
     2221 cccttcctcc agaagcccgg gaagccctcc cgggggaggg gcctgagggg agggcccttc
     2281 gcgatgaaag ggtttgggat gagctggggg aagcgtttcg ggaggtggtg gcctcggatg
     2341 cggggggacg gggggtggcc tccctttacg acccccgggc gtgggctcga ggggtggggc
     2401 tcttggcccg tagcaggcac ccggttttgg tgacggggtt cgtggtccct ccttgggggg
     2461 tggcagaaac cgatggtccc ccgggggtgg ccgttctggc ccgggcccta aaggggcggg
     2521 gcgtggcccc tcgggtggtc accgacgacc gctgccttcc cgtggtgcgg gcggcctgcg
     2581 aggccctggg aggggcccga cttcaggggt cccatgccgg ggatggggtg ctggagcccg
     2641 attccgacct cctggtcttc gtggagcgcc tggggagggc ggaagacggg gggtactaca
     2701 acatgcgcgg cgaggagatc ggcgcatgga cggcgcccct ggacgacgcc gccgccctgg
     2761 cccgcgcccg gggcatcccc gtggtggcgg tgggagacgg ggggaacgag gcgggcatgg
     2821 gaaactacgc ccgggagctc ggggccctgg tgcccgcctt ctcccggtgc ctctcggtgg
     2881 ttcccgccga tgccacgctg gtggtggacg tgtccaactg gggggcctac atcctggcgg
     2941 cgggcctttc tctggaagag gggcggtggc tggggcacga cgaggaagaa gaggaggccc
     3001 tgggagaggc catggtggca gcgggggccg tggacggggc caccaagcgc cccgaggcca
     3061 ccgtggacgg gctgccgccg gagcggcacc gaggggtggt gcggcggatc agggagctct
     3121 gggagcggcc ccggaggagc ttttagggta tgctcttggg gtatgtagtg aagggccccg
     3181 gggtcggtcg gggggatccc ggggcccgaa agctactact agagggatcc cttctggaga
     3241 aaaggcaccc gccgggccag cacggggacg aggatgagag cgaaggccgc tcccgcgccg
     3301 gtctgcagga tatcggtccc caactccacc ggggcggcgg cccacccgta gagtactccg
     3361 gcggacaggg tgtacccggt gatcatgacg acggcaccgc cgaggagggc cttcaggcgc
     3421 cccttggggg ccagggtgcc caccacgaac ccttccgctc ccttgatgat cagggtgagg
     3481 ggggcccaga gggcgtaccc caggagcagg tcggccaggg ccgcccccag cccccccgct
     3541 gcggcgccga accggcgtcc cagcagaagg gccacggtgt agattacccc ctctcccagg
     3601 ttgaaataaa gacggaatcc cggcacgggc accgatagca tggtggccac ggtgaccaag
     3661 gcggtacata cggccccgag ggccacggtg cgagcgacat ttggggaagt catggcggat
     3721 ccctcctaaa acggatgact taaaaccccg gaggaccagg gccttctgtt ccagagtata
     3781 ccgggttcct cccgggggaa acaggtccag agcccccgag gaggaggaac tttctggact
     3841 ggaacaaagg gccaagggaa gaaaacccct tgccaaaaag cgggggggaa agggtatact
     3901 agggcggtgc ggctcccctg ggggcgccgt gccggggatg tgtgccccga aggcctaaac
     3961 cggcgagggg cgagccatga gaggcccggc gccggaaggg aagaggggcc tggcttagct
     4021 ggagggatgg cttcccccgg ggcctttggg gctcggcgga gccagagtcc tttcagagct
     4081 ttttttcgtc ctttctcggg cgccatgcgg tgatcccatc ttatcatggc aaatcacaac
     4141 gaaggaggtt tgattcatgc gctttttcct ggacacggcc aaccttgacg aaatagggca
     4201 agcgatggcc tggggtgtgg tggcaggggt caccacgaac cccactctgg tatcgaaaga
     4261 gggtaacctg gactttttca ctcgggtccg cgagatcgcg gagatcgtga aggggcccgt
     4321 gagcgccgaa gtcatctccc tggacactcc gggcatggtc cgcgaggctc gggagctggc
     4381 ggccctttcc ccccatgtgg tggtgaagat ccccatgacc ccggagggca tgggggcggt
     4441 gcggatccta tctgaagaag ggataaacac taacgttacc ctggtcttct cctctcccca
     4501 ggccatcctg gcggcggcgg cgggggccac ctacgtgagc ccctttgtgg ggcgtctgga
     4561 cgacgtggga gagtcgggca cggagttgct acgggagatc gcggcgatct acgacgtcca
     4621 gggaattgag acggagatca tcgccgccag cattcgaaat cctcgccatg tggtggacgc
     4681 ggcggtggcg ggatgtcata tcgccacggt gcccttcaag gtcctccagc aacttttcga
     4741 ccaccccctc accaaatcgg gagtgaagcg attcctggag gactgggaga cctaccggag
     4801 gcaccatgcc tgagcaagaa gccgtgccgg cccagctgga gaacggtaac gccgaagggg
     4861 gcgaggcccg tccccgcccc cgacattcct tcagcgcctt ggcgggactc aacgtcccgg
     4921 agttacgtcg attggcccgg gagctggagg tctccggtgc gtcttcgctg cgcaaggacg
     4981 acctgatcgt ggcggtcctc aaggcccagt ccgagcgcat gggattccgg ttcggcggag
     5041 gcaccttgga aatcctggcc gaagggtacg gctttttgcg ccccaagggc cttctcccca
     5101 gcgatcacga tatctacgtg tccgcttccc aggtgcgacg tttcggcttg cgcaacggag
     5161 acgtggtgtg gggcgtggtg cgagctccca aggaagcgga gcactacgag gccctgcttc
     5221 gggtggagct ggtgaacttc accgaccccg aaatcgcccg gaaacgccct cagttcgaac
     5281 acctggtgcc catattcccc aacgaacggc tgcgactgga aacggaacag aagatcatct
     5341 ccacccggct ggtggatctc ttcgccccca tcggcaaggg acagcgggcc ctcctggtat
     5401 cccctcccaa ggcgggaaag accaccctcc tcaagcagtt ggcccacgcc atcaccgaca
     5461 atcatcagga cgtgatcctc atggtgctcc tcatcgacga gcgccccgag gaggtcacgg
     5521 acttctcccg ggcggtggac ggcgaagtca tcgcctccac cttcgaccgc cccgtggaag
     5581 agcacatgag ggtggcgaac ctcacgctgg agaaggccaa gcgcctggtg gaagcgggca
     5641 aggacgtggt gcttctgctg gactccatca cccgcatggc ccgggcgtca aacctggtgg
     5701 tgcccccctc ggggcggacc ctctccgggg gcatggaccc cgcaggactc tactttccca
     5761 agcgattctt cggcgccgcc cggaacatcg aaaacggcgg cagcctcacc atcatcgcca
     5821 cggccctcat agatacgggg agccgcatgg acgacgtcat ctacgaagag ttcaagggca
     5881 cgggaaacat ggaagttcac ctttcgcgca agctctccga gcagaggatt ttcccggcca
     5941 tcgacattac ccgctccgga acccggaggg aggagctttt gctggacgaa gaggagcttc
     6001 cccgggtgtg gaccctgcgg cggcgccttt cttccgtgga cgaggccgaa gccctcagcc
     6061 tcatcatcga caaggtgcga tccaccgctt ctaatcgaga tttcctggcc accatcaagg
     6121 gagcctagtc ccgggcgcga gcgtggaagg agcggcggag gccccccagg aaccatgggg
     6181 cggcctaggg aaagccgctc gggctccacg caaaattccc ccgcctagag cgaggggaag
     6241 ggaggtatgt ggtgtgaaac gttcttttcg atttattcgc cttttgcgag gacgagggac
     6301 cttcgtgttt ttcctggtcg gcatgacggt gctcctgtcg gccacggtga tcttggcggg
     6361 aaagcgcgcc gaggagcggg gctttgctcc cttctccgtg gcgccttttc ccgacgcgac
     6421 gcaaggcgac gggtacgccc tggcggagat gccccaggat gacgccgagg gcatgcccct
     6481 tcccccggag ggaggagcca cagtggccct catggggggc ctcctctcag gagacgaagg
     6541 aatcgagatc ggccccttcc aggcgccgga ctttttcctt tccgacgacg tgatcctgga
     6601 ggaaaagggc ccctcggacg aggaagggca ttcccttctg ggcccgagga aggccttgga
     6661 cgaccggccg gtctgggagc agcacgaagt ggcgccggga gataccctgt cttccatcgc
     6721 caagaagtac ggagtgtccc aggagtgcat cgcccaggca aaccccacca tgaaaaaccg
     6781 agacaggctc tccctgcgcc aggtgctcct catccctcgc tcctccgccg acatccccgc
     6841 cgtgttggag gaacaccaga ggcgccaaga cgagatcgtg gcccagaaga agtcggccaa
     6901 gcccgtggtg gtgtcctcct atgcggtggt gggaggggac agcctgtggt ccatcgccgg
     6961 aaagttcaac ctggacatca acaccctgct ggggtgtaac gaactcaagg acccggaccg
     7021 ccttcccccc ggagcgaccc tccggatccc caaccaggac gggatatttt acgtcttccg
     7081 caagggggac acgcttaaaa agatctccga ccgcttcggc atctacgaag aggctatttg
     7141 ggcggccaac tccatggacc ggggctccgt cttagaggtg ggtaagacga cgcttttttt
     7201 acccgccgcc aaacccatca ccgtggtgca gcaggccgaa gggggcagga gggactccat
     7261 ctcctctccc acgaagaagg ccggggggtt cgtgtggccc ctccaggggc gcataaatag
     7321 ctctttcgga tggcgccggg atcccttcag ccggagaaag gacttccaca cggggcttga
     7381 tatcaaggga acctacgggc gtcccatcgg ggccgccaag gctggcacgg tggtctacgc
     7441 ggggtggatg gggggctacg gccgaacggt ggtcatcaac cacggcgggg gatacaccac
     7501 gctctacgga cactgcagca agctcttggt gcgccaggga cagaaggtgg aggcccgaaa
     7561 gatcatcgcc cgatgcggct ccacggggcg atccacgggg acccacctgc acttcgaggt
     7621 gcgccgaaat aacgtcccca tcaacccgcg gagcgtgttg cggtaacttc ctcccgcagg
     7681 gacccgaagc catgatcggc caggggaacc cctcccctgg ccttgtgcgt gaaaggcagg
     7741 gcggaagtag aggcatggaa ggtccgagat gggctcatca gcgtctgcac gatcgatgga
     7801 gtaccctgct cctgcgcttt ggcttttccc agggagtgac cccctgcctg ggggccctgc
     7861 ggcacacggg aggagcctcg ggggcgatgg tgtgcgccgc ccccgaggtg gccgaggcga
     7921 tgcctcagga ggaaaggtcc accccctcgg agggcggagc cctctcttcg gggtggcgcc
     7981 tggtggcctc taggggagtg gcgacccccc accaggacct ggagcgcctg ctccccgccc
     8041 tgggacgtct ggaaggagcc cgctttcccg tgcgcctctc cctggccaag ggcccctggg
     8101 ttttgctccc cgtgcgggat ggagagacct tcctcggagg gctggccctc tggaccctct
     8161 gggggcggag catcgctgtg ggggattttg cttacctcca ggagctggcc gccctgttgg
     8221 ggaaggcctg gggaagagag caccacatgg ccgtgctcca tcgggccctg cggctcaccg
     8281 ccgccgccaa cttggtgggg ggactcctgg aaaacgaggg aattacgccc caggccctct
     8341 tttcttcggc cctggagcag gtgcgccggg tgattccctt tgaccggtgc tccatata
//
LOCUS       DAOOER010000075         7928 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_9404_length_7928_cov_8.289470, whole genome shotgun sequence.
ACCESSION   DAOOER010000075 DAOOER010000000
VERSION     DAOOER010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 7928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7928
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9404_length_7928_cov_8.289470"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PL162_08305"
     CDS             complement(<1..286)
                     /locus_tag="PL162_08305"
                     /inference="COORDINATES: protein motif:HMM:NF020050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged-helix domain-containing protein"
                     /protein_id="HQF92057.1"
                     /translation="MSHPPLTEPEYALIALLGEETMPLGAGALRLALETRGFHLSEAT
                     AGRILQRLDRLGYTERCGFQGRRLSSEGRRLYEAHRILQHRMQSARGVMSA"
     gene            520..1662
                     /locus_tag="PL162_08310"
     CDS             520..1662
                     /locus_tag="PL162_08310"
                     /inference="COORDINATES: protein motif:HMM:NF020084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HQF92058.1"
                     /translation="MRVTQNLWMSGQGWRDRWEDGAELPPERSFILAVGDGEAWENRA
                     WREELRGLFPEVSLVGCSAAGEIEGEDLREGVVAVTAVSFDATTVRAISTPLEALEGS
                     FRAGHFLGRALEAPHLRHVLVLTDGITVQGADFAQGLSEALPEGVRASGGMAGDGARS
                     QCTTLLEGDVLAPGRALALGFYGPSLGVGCGVFGGWSPFGPPRTISRSQGAFLAELDG
                     RNALDLLRQYVGDEVLDDERLMSFPLGVCDDQGKVVAVRALFRADPHGRRVVTVGEIP
                     EGTRVRFLRGSAESVLDGAREAARASLESLGRAPEWALAVGCYGRKCFLGPRAEEEIL
                     EVAGALGGSVPLAGFLSYGELAPVSGMGRGCLFHNETMTVTAFAED"
     gene            1685..>3406
                     /locus_tag="PL162_08315"
     CDS             1685..>3406
                     /locus_tag="PL162_08315"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQF92059.1"
                     /translation="MEEWHPLLDRQCRRYLAAERDNLSLEGFLRAVDSAYRGFDVERS
                     QLERFLDAASTELASCNEEMRTVFQLFPDLFFRLDGEGRILDYTLGMVSGPTLVRQTD
                     RVERVEDVVPGEAGEKLVKAWRQARRQEGVVTMEFDTMAGQTPLFYEAIFIPRGEQCL
                     LFLRDIGSRRMAEDLLRCQNESLRVLHDAGMALMERRTLGDLCEVLVRRAAALLEVED
                     AYLRLHDPSTGQWKLAFASGRVTQFCDPSVAHLWGISLEVSRSKSVFYLPDYDAWEGR
                     NLKIPPGTYGSVLGAPIVSSGETKGILCLLGKPGKVFGTEQIFLLDQFVRTAALAIEN
                     VRLGEEVREELEERRRAEQEVRHLNATLEERVSERTAQMEDMNRELEEEIAEKVAMEH
                     LLEENLTRVRAALEETVNALTATTEKRDPFTAGHQRMVAHLACAIAQRLGMSSDRIEV
                     LRTAGLLHDIGKISVPIEILSKPGRLTAEEMRLIHPHSTVGYEILARIPFDGPVAEMV
                     WQHHERLDGSGYPRGLRGEDILLEARILAVADVVEAMASHRPYRPSLGIRAALQEVVR
                     HRGTAFDP"
     assembly_gap    3407..3506
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3646..4266
                     /locus_tag="PL162_08320"
     CDS             3646..4266
                     /locus_tag="PL162_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQF92060.1"
                     /translation="MEWMQAGGGIMWAIAALSVLAGAVVLERLWFFSRSWADPEELEE
                     RLGRALYDNDPEGVRRVVSGGRSSLHRLFSAATSHWQVDAATLKLLLEQEIRRELYRW
                     ERGLALVATVGRLAPLLGLLGTVLGMVEIFQTLPSAREAPMVALAGGIWKALLTTVAG
                     LVVAVPAVLFHTFLLSRVDRIEETLERGADFVLREKLLGRGADRGA"
     gene            4263..4661
                     /locus_tag="PL162_08325"
     CDS             4263..4661
                     /locus_tag="PL162_08325"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQF92061.1"
                     /translation="MTVRPRRSRGGEVDLTPLIDVLFILVLFLVLAGSMDRSSLAVNL
                     PEGRGSQGSGPVPWTVSVLRDGSLVDDGGRGCDPEEALKGALEAERARRPVRIAGDRG
                     APYGAVAELLARFRAGGVTAVELVFEGGQD"
     gene            4710..4994
                     /locus_tag="PL162_08330"
     CDS             4710..4994
                     /locus_tag="PL162_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HQF92062.1"
                     /translation="MVHGEFGGLLLGGLLLLLNVGAVLVMGWDKFQAQRQGRRVPERT
                     LFLLALGGGSVGCLAGMYGFRHKTRHASFVWGMPLILLAQLSLALALFWR"
     gene            5046..5903
                     /locus_tag="PL162_08335"
     CDS             5046..5903
                     /locus_tag="PL162_08335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HQF92063.1"
                     /translation="MREPSIFWLISLGIHAFVIGLLGEWGPSPAKTLPETPGIPVRLR
                     LVSSGGAEGRPLALARPRPTPVLRTHPADERPPRRLVSRGERALPPAPEPRERAAVSP
                     PPRRPAKPSPAAVPSPVPKDPKGRKKDSSALSPAPRPSSPPQAPSRAPSGAPRGEGLS
                     RSSDRPGLSPEDRGAVGRPSPGPGGPDGEVSTVRGRFPLVEASSLRVTRQVTPEYPPA
                     SRRRGEEGTVVLILTVEGGRVKHAEVERSSGFRALDEAAEKALRRWRFDAPGEVRVRV
                     PVAFHLETE"
     gene            complement(5912..6544)
                     /locus_tag="PL162_08340"
     CDS             complement(5912..6544)
                     /locus_tag="PL162_08340"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HQF92064.1"
                     /translation="MRRSPLGPVLLKALLGMMIWGGGIGESFPGEGALVVPLVVPEED
                     PSGGPAPFSSEELMEARIRQGLLFYNRRLHPAAARAYGEYAVAYGRMCRIDPFLLAGV
                     LVVESRGDPEARSPVAHGLMQINWEVHKDLIRSRFPFIKTAEDARIPSNNLLLGAFIL
                     RRYLDACGGSEEEALYRYLGARSPRYAAAVQEARDRLASTDPLFLVRLDP"
     gene            complement(6541..7293)
                     /locus_tag="PL162_08345"
     CDS             complement(6541..7293)
                     /locus_tag="PL162_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92065.1"
                     /translation="MTRSNPSRRHMGIFLGVSALGGALALAPVVLGIDMSSGGGAMIL
                     LGIALIFVGILMALFLRKRAQILDRLLSEKDLLAHWTYTPDEWEAYAKKDLLFRSSHS
                     KKTFFLIAFFAVLFGMVFFIADPEAGRFVALGLAAVMLLMAVVAKLSVDRARRRQTQE
                     AKELLLSAEGLWLAGELHTWTVGGARLEGVTYAPEDRKILSFTYSTPGKNGPQYYTVR
                     VLIPRGKEEEGAAVEAFFRDPERTRPQKGHRS"
     gene            7460..>7928
                     /locus_tag="PL162_08350"
     CDS             7460..>7928
                     /locus_tag="PL162_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92066.1"
                     /translation="MGLEMVLLLFEASRPCWDRENVELAGEDPAGLEALFREGLLEER
                     DGVYHLSPQGRERFRSEATACYLDAEPGALPEDGGRVVRRHRWALLLDRAFVGRWGEK
                     VFRPGASLPYLPPRHEGPLGRREGEDFSWLYPRDPFFEEMDRRFPAPREAPRAP"
BASE COUNT         1141 a         2313 c         2975 g         1399 t
ORIGIN      
        1 gggcggacat gaccccccgg gcggattgca tccggtgctg gaggatgcga tgggcttcgt
       61 aaaggcggcg accctcggag gaaaggcggc gtccctgaaa gccgcagcgt tcggtgtagc
      121 cgaggcggtc caggcgctgt aaaatgcgcc ccgccgtggc ttcgctcagg tgaaatcccc
      181 gggtttccaa ggcgagacga agggcccctg cccccagggg catcgtttct tcgcccagca
      241 gggcgataag ggcgtattcg ggctctgtca ggggagggtg ggacacggtg ggtctccttt
      301 cgggctcagg ctgcccatca ttggtgagtg gcgtggagga ttatggccga tccggcgggg
      361 tctgtcaagg gggccggggg attcctggcg tcgatgggaa aagatggggg gcgattttct
      421 ccttgtctct cctggccgtt ccttctatac tgggcgaagt ggcaagggag agggtgaagg
      481 tgaaggggtg agcctgacac ctcgaagggg ggaagggccg tgcgggtgac gcaaaatctc
      541 tggatgtccg gccaagggtg gcgggatcgc tgggaagatg gggcggagct ccctccagag
      601 cggagcttca tcttggccgt gggggatggg gaggcctggg agaatcgggc ttggcgcgag
      661 gagctccggg ggctttttcc cgaggtttct ctggtggggt gttctgcggc gggggagatc
      721 gagggggaag acctccggga gggcgtcgtg gccgtcacgg cggtgtcttt cgacgccacg
      781 acggtgcggg cgatctccac gcccctggag gcgctggagg ggagctttcg ggcggggcac
      841 ttcctggggc gggccctgga ggctccccat ctgcgtcacg tcctggttct caccgacggc
      901 atcacggttc agggggcgga cttcgcccag gggctctccg aggccctccc cgaaggggtg
      961 agggcgagcg ggggcatggc gggggacggg gctcgaagcc agtgcaccac cctgctggaa
     1021 ggagacgtcc tggccccggg gcgggccctg gccctggggt tttatggccc ttccctgggg
     1081 gtggggtgcg gcgtctttgg gggatggtcc ccctttggcc ccccccggac aatttcccgg
     1141 tctcaagggg cctttctcgc cgagctggac gggcgcaacg ccctggacct ccttcgccaa
     1201 tatgtggggg acgaggtcct tgatgacgaa cggctcatgt cctttccctt gggggtctgc
     1261 gacgaccagg ggaaggtggt ggcggtgcgg gcgctgtttc gcgccgatcc ccacgggcgc
     1321 cgggtggtca cggtggggga gatccccgag ggcactcggg tgcgcttcct gcggggcagc
     1381 gcggaaagcg tcctcgacgg ggcgcgggaa gcggcgcgag cgtccctcga atctctggga
     1441 cgggccccgg agtgggccct ggcggtgggg tgctacggcc ggaagtgctt tttgggaccc
     1501 cgggcggagg aagagatcct ggaggtggcc ggggctctgg ggggctcggt gcctttggcg
     1561 gggtttttgt cctacggaga gctggccccg gtatcgggga tgggccgggg atgcctgttc
     1621 cacaacgaga ccatgaccgt gacggcgttc gccgaggact gaccagcgaa gggagagagc
     1681 tcttttggag gagtggcatc cgcttctgga ccgtcagtgc cgccgatatc ttgccgcaga
     1741 gcgagataac ctctctctgg agggcttcct tcgcgccgtg gacagcgcct atcggggctt
     1801 cgacgtcgag agatctcagc tggagcgctt cctggacgcc gcctcgacgg agctggcctc
     1861 ctgtaacgag gagatgcgca cggtcttcca gctctttccc gacctctttt tccgcctcga
     1921 cggggaaggg cggatcctgg attacaccct ggggatggtg agcggaccca ccctggtgcg
     1981 ccagacggat cgcgtggagc gggtggaaga cgtggtgccg ggggaggcgg gggaaaaact
     2041 ggtgaaggcc tggcgtcaag cccggcgcca ggagggcgtg gtcaccatgg agttcgacac
     2101 gatggcggga caaacgcccc tcttctacga ggccattttc atcccccggg gggagcagtg
     2161 tctgctcttt ttgcgggaca tcgggtcgcg gcggatggcg gaagacctcc tgcgctgtca
     2221 aaacgaatcc ctgcgcgttc tccacgacgc cgggatggcc ctcatggagc ggaggacctt
     2281 gggggatctc tgcgaggtgc tggtgcggcg ggcggcggcc ctgctggagg tggaagacgc
     2341 ctatctccgg ctccatgacc cctccacggg gcagtggaag ctggcctttg cctcggggag
     2401 agtgacccag ttctgcgatc cctcggtggc ccatctttgg gggatttccc tggaggtgtc
     2461 ccggtcaaag agcgtctttt atttgcccga ttacgacgct tgggaggggc gcaacctcaa
     2521 gatccccccg gggacctacg gctcggtcct gggggctccc atcgtgtcgt cgggagaaac
     2581 gaaagggatc ctatgtcttc tgggaaaacc ggggaaggtc ttcggcacgg agcagatctt
     2641 tttgctggat caatttgtcc gcaccgccgc cctggccata gagaacgtgc gcctgggcga
     2701 agaggtccga gaggagctgg aggagcgtcg ccgggcggag caagaggtac gccacctcaa
     2761 cgccacgctg gaggagcggg tctcggagcg cacggcccag atggaggaca tgaaccggga
     2821 gctggaagaa gagatcgccg agaaggtggc gatggagcat ctcctggagg agaacctgac
     2881 ccgggtccgg gcggcgctgg aggagacggt gaacgccctc acggccacca cggaaaagcg
     2941 cgaccccttc acggcggggc accagcgcat ggtggcccac ctggcttgtg ccatagccca
     3001 gcgtctgggg atgtcttcgg atcgcatcga ggtgctgcgc acggcggggt tgcttcacga
     3061 catcggaaag atctccgtgc ccatcgaaat cctgagcaag ccggggcggc tgacggcgga
     3121 agagatgcgg ctcatccacc cccactccac cgtggggtac gagatcctgg ctcggattcc
     3181 cttcgatggc cccgtggcgg agatggtgtg gcagcaccac gagcgtctgg acggctcggg
     3241 gtatccccgg gggcttcggg gggaggatat cctcctggag gcccggatcc tcgccgtggc
     3301 ggacgtggtg gaggccatgg cgtcccaccg cccctaccgt ccctcgctgg gcatccgggc
     3361 ggcgctccaa gaggtggtgc ggcaccgggg aacggccttc gaccccnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnngcgc gctatttcgc cccctccggc cttcgccggg
     3541 gtcttttgac atctgggagc gagggggcta tagtgctccg gagtcggcgg ggatttgaac
     3601 ccgaaggggc cttcgggggt tttgaggatg gggcgggggg ttttcatgga gtggatgcag
     3661 gcgggagggg gcatcatgtg ggccatcgcc gccctgtcgg tgctggcggg ggcggtggtg
     3721 ctggagcggc tgtggttttt ttctcggtcc tgggcggatc ccgaagagtt ggaagagcgc
     3781 ctgggccggg ccctgtacga taatgacccg gagggggtgc gccgggtggt ttccgggggg
     3841 cggtcttccc tccataggct tttttccgcc gccacgagtc actggcaggt ggatgccgcc
     3901 acgctgaagc tcctgttgga gcaggagatc cggcgggagc tctaccgttg ggaaaggggg
     3961 ctggccctgg tggccaccgt ggggcgtctg gcccccctgt tggggctttt gggcaccgtg
     4021 ctcggcatgg tggagatctt tcagaccctg ccctcggccc gtgaggcccc catggtggcc
     4081 ctggcggggg ggatctggaa ggccctcctc accacggtgg cggggctggt ggtggcggtg
     4141 ccggcggtgc tctttcacac ctttcttctg tcccgggtgg atcgcatcga ggagaccctg
     4201 gagcgggggg cggacttcgt gttgcgggag aagctcctgg ggcgcggcgc cgaccggggg
     4261 gcgtgacggt gcgccctcgg cgatcccgag ggggggaggt ggacctgact cccctcatcg
     4321 acgtgctctt catcctggtg cttttcctcg tgctggcggg gagcatggac cgcagctccc
     4381 tggcggtgaa cctccccgag gggcgggggt cccagggctc gggacccgtg ccttggacgg
     4441 tgtcggtgct ccgagatggc tccctggtgg acgatggggg gcgagggtgt gaccccgaag
     4501 aagcactgaa gggagccctg gaggcggaaa gggcccggcg ccccgtgcgc atcgccggag
     4561 atcgaggagc cccctacggc gcggtggcgg agctgttggc ccggttccgg gcggggggcg
     4621 tgacggcggt ggagttggtg ttcgagggtg gccaggacta ggcgggaagg gcctacctgg
     4681 gcgcccagga gtcaagagga gggcggttag tggtgcatgg ggaatttgga ggtctccttc
     4741 tggggggact cttgctgctg ctcaacgtcg gggcggtgct ggtcatgggg tgggacaagt
     4801 tccaggccca gaggcagggg cgtcgagtgc cagagcggac gctcttcctc ctggccctgg
     4861 ggggagggag cgtggggtgt ctggcgggca tgtacggctt ccggcacaag acccggcacg
     4921 cgtccttcgt ctgggggatg cccctcatcc ttttggccca gctctccctg gcgttggcgc
     4981 tcttctggcg ctaaagggtc cggggggagc ggcgtcggaa ccttggtcaa cggggggcgc
     5041 cagaggtgag ggaaccttcg atcttttggc tcatcagcct ggggatccac gccttcgtga
     5101 tcgggctttt gggggagtgg ggcccctctc cggctaaaac gcttcccgag acccccggga
     5161 tccccgtgcg gctgcgtctc gtgtcctccg gcggggcgga ggggcgccct ttggccttgg
     5221 cgcgcccccg tcctaccccg gtcctgcgga cccatcccgc cgacgagagg ccccctcggc
     5281 gcctcgtctc acggggggag cgggcgctcc ctccggcccc cgagccccgg gagcgggcgg
     5341 cggtgagccc tcccccccgg cgtccggcga agccctctcc ggcagcggtt ccctctccgg
     5401 tgcccaagga tcccaagggg cgaaagaagg attcctcggc cctatccccg gctccccggc
     5461 cctcgtcccc gccccaggcc ccttcccggg ctccttcggg ggccccgcga ggagaggggc
     5521 tttcccgctc ctccgaccgg cccgggcttt ctccggagga tcggggggcg gtggggcgtc
     5581 cttccccggg gcccggagga cccgacggag aggtgtccac ggtgcggggg cgctttcccc
     5641 tggtggaggc ctcttcgttg cgggtgacgc gccaagtgac tccggagtat cccccggcga
     5701 gtcgtcgtag gggggaggag ggtacggtgg tgctgatcct cacggtggag ggggggcggg
     5761 tgaagcacgc ggaggtggag cgatccagcg gttttcgagc cctggatgag gcggcggaaa
     5821 aggccctgcg ccggtggcgt ttcgacgccc cgggggaggt gcgggtgcgc gtccccgtgg
     5881 cctttcatct ggagacggag taggtcctcc ttcaggggtc caggcgcacc aggaagaggg
     5941 ggtcggtgga agccagccga tcgcgggctt cctgcaccgc cgctgcgtac ctaggggacc
     6001 gggcccccag gtatcggtag agggcctctt cttcgctgcc tccgcaggcg tccaggtagc
     6061 gacgaaggat gaaggccccc agcaggaggt tgttgctggg gatgcgggcg tcttccgccg
     6121 tcttgatgaa ggggaatcgg gatcggatga ggtctttatg gacttcccag tttatctgca
     6181 tgaggccgtg ggccacgggg gatcgggctt cgggatcccc ccgggattcc accaccaaca
     6241 ccccggctag caggaagggg tctatgcggc acatgcgccc gtaggccacg gcgtattctc
     6301 cgtaggcccg ggcggcggcg gggtgcaggc ggcggttgta aaagagaagt ccctgacgga
     6361 tgcgggcctc catgagctct tccgaggaga agggggcggg cccccccgag gggtcttcct
     6421 ccgggacgac cagcgggacg accagggccc cttcgccggg gaagctctcc ccgatccctc
     6481 ctccccagat catcatcccc aggagggcct ttaggaggac cgggccgagg ggggaacgcc
     6541 tcaacttcga tggccctttt ggggtcgggt ccgctccgga tccctgaaaa aggcttccac
     6601 cgccgctcct tcttcctctt ttccccgggg aatgagaacc cgtacggtgt agtactgggg
     6661 accattcttg ccgggggtgg agtaggtgaa cgagaggatt ttcctgtctt cgggggcata
     6721 ggtcacccct tccaggcggg cccctcccac agtccaggtg tgaagctctc cggcgagcca
     6781 gagcccctcg gcggaaagga gcagctcctt cgcctcttgg gtttgcctcc gccgggcccg
     6841 gtccacggag agtttggcca ccaccgccat gaggagcatc acggccgcga ggcccagcgc
     6901 cacgaagcgg ccggcctcgg gatccgcgat gaagaagacc atcccgaaga gtacggcgaa
     6961 gaaggcgatg aggaagaagg tctttttcga gtggctgctc cggaaaagga ggtctttctt
     7021 ggcgtaggcc tcccactcgt cgggggtgta ggtccagtgg gccaggaggt ctttttccga
     7081 taggagccgg tccaggatct gggcccgttt ccggaggaac aatgccatca ggatccccac
     7141 gaagatcagg gcgatcccca ggaggatcat ggcgccccct ccggaggaca tgtcgatgcc
     7201 taagaccacg ggggccaggg ccagagctcc tcccaatgcg gagaccccga ggaaaattcc
     7261 catgtggcgc cgagacggat tggagcgggt cacgtttttt tcctcccttg cgtggggctt
     7321 cccggggact tcgaaaaggc ccgggggcgg atggagcctc ctcattatat cgcatcagaa
     7381 gggagaatag taggggcgaa ggcccctttt tcgagagccg ggggcgaaaa ggagaaaaga
     7441 tggggggagg aggtccgcca tgggcctaga gatggtgctt ttgctttttg aggcctcacg
     7501 tccctgctgg gaccgagaaa acgtcgagct ggcgggggag gatcccgccg ggctggaggc
     7561 cctcttccgg gaggggcttt tggaggagcg ggacggggtg taccacctct ctccccaagg
     7621 gagggagcgc tttcgcagcg aggcgacggc gtgctatctt gatgccgaac ccggagccct
     7681 tccggaggac ggggggaggg tcgtgcggcg tcaccgatgg gccctcctgt tggaccgggc
     7741 ctttgtgggg cgatgggggg agaaggtctt tcgccccggg gcctccctgc cctatttgcc
     7801 ccctcggcac gagggccccc tggggcgccg ggagggggag gatttctcct ggctttaccc
     7861 ccgagatccc tttttcgagg agatggatcg acgctttccc gccccccggg aggctccccg
     7921 ggcccccc
//
LOCUS       DAOOER010000076         7406 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_10158_length_7406_cov_9.578833, whole genome shotgun sequence.
ACCESSION   DAOOER010000076 DAOOER010000000
VERSION     DAOOER010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 7406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7406
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10158_length_7406_cov_9.578833"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3149
                     /locus_tag="PL162_08355"
     CDS             <1..3149
                     /locus_tag="PL162_08355"
                     /inference="COORDINATES: protein motif:HMM:NF024947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HQF92067.1"
                     /translation="ITGTGIYTEISQRVHERRGEEQKKLDALDSVLQSTKIPDEGQAA
                     AWRHDLEEKKTEEPRLRQDMEDKKHRLDLHDRLAHAEEELEKGEASLQEWTNRAEAFA
                     PQEERLARALQALELASLHGALERLRKDRRAESDLLEDLRLRLPELDEALHQADEAWQ
                     IAQASLQDLKAREATLRPLWQRVRGQDLQLQEWEKVLGTREAERETQKKNLETLEENR
                     TKEQRLLDEETATRKTLERELEQNAADGALGEALGALLERLDALQDLSRQSARRYQEE
                     AEAENLHAAAVAERQRRDQLLQERQQALATLEDAVARRRKALEDLLQGRSLGEWRQSL
                     ERLRERRALLDQGIQTLQLREKHQLLLENARQRAQELTDGEGALKEDRQRTEARLETL
                     ARETELLEIQRDLLLRIQALEDQRKELRDGEPCPLCGAREHPLAWGQLPPPYAADDDL
                     ARSKRELQELHDRRARITNQQGAVQKDLERNVQDQEEARQSIAEAEQSLRRIWEQRGE
                     EVPQDRWEAHAELRRREEDEALRRTEDTVIRGDTEEHELAKRGQDREELREFAEAAKL
                     NAQKAAHDEASALQALEAARRARSAHATNVDQARRDLRQRLAPWNDASLAEETLEDVR
                     QTLRARKERWEQHHNEKIRLDQTIQMGHHALEQWTLRIREATDTLARQDADLEARRGE
                     HRLLEEERKNLFQDRHPDEEEQQFVEALTRAEETLALRRRDKDQALEGRNRAQDRRDT
                     LNRSLENRDRDLASEEERWTRRLQEGAFLHEEDFLAANLEEPQRRALAEASQLLKEEG
                     AARKSTLERQRRQRDELRELQGDPVLREELLREIADLETLHGEAQRAVGVLEEKLRDL
                     DTLRQEHRDQQTRCEAQRRELRRWENLHHLVGSADGKKFRNFAQGLTFRAMIGHANHQ
                     LRNMTDRYVLVQERQAPLELAVVDTYQGGVARSTKTLSGGESFLVSLALALGLSHMVS
                     RRVRVDSLFLDEGFGTLDDDALDTALETLAGLHRSGKLIGVISHVPAIRERLAARIQV
                     IPGPGGKSALQGPGCGAK"
     gene            3227..4612
                     /locus_tag="PL162_08360"
     CDS             3227..4612
                     /locus_tag="PL162_08360"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HQF92068.1"
                     /translation="MRTLKVFGILLWAAALLVPLRGNAAPPPTEYIILYRDGTLALLT
                     DRPDRTPHAVSPHTAQALLKAKPYLVPQGDFSSRLEAARADPDVRYLEPNFPVELLEV
                     PNDEYYPMFQSKIMESLDLPGAWHHTTGNADVIVAVMDTGMQGSHPDLGPNLWVNPGE
                     IPANGVDDDRNGYVDDVNGYNTFDDNGNASDDLFGHGTSVAGIIGAVGNNGFGVAGMN
                     WTVRMISVRMWGSDPRAPSDRYNVMKAYQYVLALKRHGVNIRVVNASFGTSEGGPGGM
                     EEHFVRDLEAENVLLVAASGNDGRNTDATPVYPACFSADNVVSVGGVDPQTWTRCSFS
                     NYGARTVDLFAPGNGVLTTGREFNFRVFLGTSSAAPFVTGLGALLWAASPDASMAQVR
                     DALLKGVTLSDAFAGKCVSGGILNARGSMEQLLSPPAPQRSSSGGGCQGFEASGALLL
                     GALPLLWRRKR"
     gene            4719..5459
                     /locus_tag="PL162_08365"
     CDS             4719..5459
                     /locus_tag="PL162_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HQF92069.1"
                     /translation="MKRLLMALLVGLLAASGAGAEGKKITAAADPWPPFTDPRSPTQG
                     LSMEILRAAVGPQGYEVELRFMPWTRAEERVTAGEIDVIPSTWTTEERRRRLLFSEPY
                     AINEVKFVKRKGDPFEYEGIESLSGKTVGIVRGYGYPEAFKGATNFRRREANDTLSNV
                     KRLVFGLVDLTLEDRIVLVDTLRKSDPALLDEIELTKNSLSTQTLSISCAIVNPRCQE
                     IIDAFNRGLEEIKADGTLDRVLAGYRLK"
     gene            complement(5531..6889)
                     /locus_tag="PL162_08370"
     CDS             complement(5531..6889)
                     /locus_tag="PL162_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQF92070.1"
                     /translation="MPHAQSQFLKMTTVPVGRLISSLAVPTIISMLVTSIYNMADTYF
                     VSQIGTSASGAVGVVFSLMAIIQAVGFTIGMGAGTWISRLLGARLLDEARSVASSAYY
                     LSLLLGGAVAVGGLGAIDPLMRLLGATDTILPYAKEYASYILYAAPIMAASFLLNNIL
                     RAEGRAQFAMIGITAGGILNIFLDPLFIFTFRLGIAGAAIATALSQCVSFSLLLSGFL
                     THRTQTSLHVRYVAKAPALYGQIVKNGLPSFCRQGLASVAAIATNLSAAVYGDAAVAA
                     LSIVGKVFMVAFSVVIGVGQGYQPVLGYNYGAGNFDRVRGAFLFTFKTATALMVLCAL
                     GGWFGAPAIMTWFIASDPQVIAIGTGTLRAMGLAMPLVPLMVVCNMSFQSIGKSWTSL
                     LLSSMRQGFIFLPLIVLLPRFFGLPGLQVAQPVADVCTFLVCLPFARHFFDDLKRREG
                     GV"
BASE COUNT         1544 a         2422 c         2439 g         1001 t
ORIGIN      
        1 agatcacggg gaccggcatc tacaccgaga tttctcagcg cgtgcacgaa cgccgggggg
       61 aggaacagaa aaaacttgac gccctcgaca gcgttctgca gagcacgaag atccccgacg
      121 aagggcaggc agcggcgtgg cgccacgacc tggaggaaaa aaagaccgaa gagccccgcc
      181 tccgccagga catggaggac aagaagcacc gcctggacct ccacgaccgt ctggcccacg
      241 ccgaggaaga actggagaag ggcgaagcgt cgctccagga atggacgaac cgggccgaag
      301 ccttcgcccc ccaggaagag cgcctggccc gggccctcca ggcgctggaa ttggcgagcc
      361 tgcacggcgc cctggagcgc ctccgaaaag accgacgtgc ggagagcgac cttctggaag
      421 accttcggct ccgcctcccc gaactcgacg aagccctgca ccaggcggac gaggcgtggc
      481 aaatagccca agcctccctc caggacctca aggcccggga ggccaccctc cgccccctgt
      541 ggcagcgcgt ccggggacaa gacctccagc tccaggaatg ggaaaaggtc ctgggcaccc
      601 gggaggcgga acgggaaacg caaaaaaaga atctggaaac cctggaggaa aaccggacga
      661 aagaacaacg cctcctcgac gaggagaccg ccacccgaaa gacgctggag cgcgagctgg
      721 agcaaaacgc cgccgacggc gccctggggg aagccctggg agccctcctc gaacggttag
      781 acgcccttca ggacctctct cgccagagcg cccgccggta ccaggaggag gcggaggcgg
      841 aaaacctcca cgccgccgcc gtcgccgagc gccagcgccg ggaccagctc cttcaggagc
      901 ggcaacaagc cctcgccacc ctggaggacg ccgtggcccg gaggagaaaa gccctggagg
      961 atctcctcca agggcgttct ctgggagaat ggcggcaaag tctggagcgc ctgcgggaac
     1021 gccgcgccct cctcgaccag ggcatccaga ccctccagct ccgggaaaaa caccagctcc
     1081 tgctggaaaa cgcccggcag cgcgcccagg aactcaccga cggcgagggc gccctgaagg
     1141 aagatcggca acgcaccgaa gcgcgtctgg aaacgctggc ccgggagacc gaacttctgg
     1201 agatccagag ggacctgctc ctgcgcatcc aggccctgga ggaccagcgg aaagagttgc
     1261 gggacggaga accctgcccc ctgtgcggcg cccgggaaca ccccctcgcc tggggccagc
     1321 tccctccccc ctacgccgcc gacgacgacc tcgcccggag caagcgggag ctccaggagc
     1381 tccacgaccg ccgggcccgg atcacgaacc agcaaggcgc cgtgcagaaa gatctggaga
     1441 ggaacgtcca agaccaggaa gaggcccggc aaagcatcgc cgaggccgag caatctctcc
     1501 gccgcatctg ggagcaacgg ggcgaagagg ttccccagga tcgctgggaa gcccacgccg
     1561 aactccggcg gcgcgaggag gacgaagccc tgcgccgcac cgaggacacc gtcatccggg
     1621 gggacaccga agaacacgag ctggcgaagc ggggacagga tcgggaagaa ctccgggaat
     1681 tcgcggaagc cgcgaagctc aatgcccaga aggcggctca cgacgaggcc tccgccctcc
     1741 aggccctgga agccgcccgg agggcaagga gcgcccacgc caccaacgtg gaccaggccc
     1801 ggcgggacct ccgccaacgc ctcgccccct ggaacgacgc atccctcgcc gaggaaaccc
     1861 tggaggacgt ccgccaaacc ctccgcgccc gcaaagagcg gtgggaacag caccacaacg
     1921 aaaagatccg cctcgaccag accatccaga tgggacacca cgccctggaa cagtggacac
     1981 tccggatccg cgaggccacc gacaccctgg cccgccagga tgcggacctg gaggcccgcc
     2041 ggggagagca ccgccttctg gaggaggaac ggaaaaacct ctttcaggac cgccaccccg
     2101 acgaggaaga gcaacagttt gtcgaggccc tcacccgagc ggaagagacc ctggccctcc
     2161 gccgcaggga caaagaccag gccctggaag ggcgaaaccg ggcccaggac cgccgggaca
     2221 ccctcaaccg ttctctggag aatcgggacc gagacctcgc ctccgaagag gagcgttgga
     2281 cccgacgcct tcaggaaggg gcctttctcc acgaagagga cttcctggcg gcgaacctgg
     2341 aggaacccca acggcgcgcc ctcgccgagg cgtcgcaact cctgaaggaa gaaggggccg
     2401 cccggaaaag caccctggag cgccagcggc ggcagcgcga cgaactgcgg gaactccagg
     2461 gagatcccgt gctccgggaa gaactcctgc gggagatcgc cgacctcgaa acgctccacg
     2521 gcgaagcaca gcgggccgtg ggagtccttg aggaaaagct ccgggacctc gacaccctcc
     2581 ggcaagagca ccgggaccag caaacccggt gcgaggccca acggcgggaa cttcgccggt
     2641 gggaaaacct gcaccacctc gtgggatccg ccgacggcaa gaagttccgc aacttcgccc
     2701 agggattgac ctttcgcgcc atgatcggcc acgccaatca ccagctccgg aacatgacgg
     2761 accgctacgt cctcgtgcag gagcgccagg ctcctctgga actcgccgtg gtggacacct
     2821 accagggggg cgtggcccgt tccaccaaaa ccctctccgg cggcgagagt tttctcgtga
     2881 gcctggccct ggcgctgggg ctctcccaca tggtgagccg ccgggtgcgg gtggactccc
     2941 tctttctgga cgaaggcttc ggcaccctgg acgacgacgc cctggacacc gccctggaaa
     3001 ccctggcggg attgcatcgg agcggcaaac tcatcggcgt catctcccac gtccccgcca
     3061 tccgggaacg cctcgccgcc cgcattcagg tgatccccgg cccggggggc aagagtgccc
     3121 tccagggacc gggatgcggc gcgaagtagg gcaacgcccc caaaggccct tcctgcgggg
     3181 ataccccccc gggatcggaa actcacttct caaggaggaa tgccccatgc gaacgctcaa
     3241 agtttttgga atcctgctct gggccgccgc ccttttggtc cccctccggg ggaacgccgc
     3301 accgcccccg acggagtaca tcatcctgta ccgggacggc accctggccc tgctgacgga
     3361 ccgccccgac cgaacgcccc acgcggtgtc tccccacacc gcccaggccc ttctgaaggc
     3421 caagccctac ctcgtccccc agggagattt ttcgtcccgc ctcgaagcgg cccgggccga
     3481 tccggacgtg cggtacctgg agcccaactt ccccgtggag ctcctggagg tgcccaacga
     3541 cgagtactac cccatgtttc aatcgaagat catggagagc ctggaccttc ccggagcctg
     3601 gcaccacacc accgggaacg ccgatgtcat cgtggcggtg atggacaccg gcatgcaggg
     3661 atcccacccc gatctggggc cgaacctctg ggtgaacccc ggggagattc cggccaacgg
     3721 cgtggacgac gaccgcaacg gctacgtgga cgacgtgaac ggatacaaca ccttcgacga
     3781 caacgggaac gcctcggacg acctcttcgg ccacggcacc tccgtggcgg ggatcatcgg
     3841 ggccgtgggg aacaacggct tcggcgtggc ggggatgaac tggacggtgc ggatgatctc
     3901 cgtgcggatg tggggatccg acccccgggc gccatcggac cgatacaacg tcatgaaggc
     3961 ctatcagtat gtgttggcgc tgaaacgaca cggggtaaat atccgggtgg tgaacgcctc
     4021 tttcgggact tcggagggag ggcccggagg catggaggag cactttgtcc gggatctgga
     4081 ggccgaaaac gtccttttgg tggcggcttc ggggaacgac ggccgaaaca ccgacgccac
     4141 ccccgtgtac cccgcctgtt tttccgcgga caacgtggtg agcgtgggag gcgtggaccc
     4201 ccaaacctgg acccgatgca gcttctccaa ctacggcgcc cgcaccgtgg acctctttgc
     4261 ccccgggaac ggagttctca ccacggggcg ggaattcaac ttccgggttt ttttgggcac
     4321 cagcagcgca gcgcccttcg tcaccggcct gggggccctg ttgtgggcgg cgagtcccga
     4381 cgcctccatg gcccaggtcc gggacgccct cctcaaagga gtcaccctct ccgacgcctt
     4441 cgcggggaag tgcgtctccg gagggatcct gaacgcccgg ggaagcatgg aacagctcct
     4501 ctcccccccc gcgcctcagc gaagcagctc cgggggcggc tgccagggct tcgaggcctc
     4561 gggggccctc ctgctgggag ccctgccgct cctgtggcgg aggaaaaggt aggccggggg
     4621 cgcggaaccg ccacatcgcc ggggttctct tgacgctccg aggcctttga tgataccctg
     4681 cctttgtaaa gattagcctc ggaagggttg atgagaaaat gaaaagactt ctgatggcgc
     4741 ttctggtggg gcttctggcg gcttcggggg cgggggcgga ggggaaaaaa atcacggcgg
     4801 cggcggatcc ctggcccccc ttcaccgacc cgaggagccc cacccagggc ctttcgatgg
     4861 agatcctgcg agcggcggtg ggcccccagg gatacgaggt ggagttgcgc ttcatgccct
     4921 ggacccgggc ggaggagagg gtgacggcgg gagagatcga cgtcatcccc agcacgtgga
     4981 ccacggaaga gcgccgaagg cgccttcttt tcagcgagcc ctacgcgata aacgaggtca
     5041 aattcgtcaa gaggaagggt gaccccttcg agtacgaggg gatcgagagc ctgagcggca
     5101 agaccgtggg catcgtgcgg ggctacggct atccggaggc cttcaaaggg gccacgaact
     5161 tccggcgcag ggaggccaac gataccctga gcaacgtcaa aaggctcgtc ttcggcctcg
     5221 tcgacctgac gctggaagac cgcatcgtgc tcgtcgacac gctgagaaaa agcgatcctg
     5281 ccctgctcga cgagatcgag ctcacgaaaa actccctgtc cacccagacg ctgtcgatat
     5341 cgtgcgccat agtcaacccc cggtgccagg agatcatcga cgccttcaac cggggccttg
     5401 aggagatcaa ggccgacggc accctggacc gggtcctcgc cggttacagg ctgaagtagc
     5461 ccctaggacg atcctgcggg gagccttatc ggtcggggag gggcgcggcc ggtcctaggg
     5521 aaccgaaggg tcatacccct ccttcccggc gcttcaagtc gtcgaagaaa tgccgggcaa
     5581 aggggaggca gaccaggaag gtgcagacgt cggccaccgg ctgggccacc tggagccccg
     5641 gcaacccgaa aaatcggggc agcaggacga tcagcggcag gaagatgaag ccctggcgca
     5701 tggacgagag aagaagggac gtccaggact tgccgatgga ctggaaggac atgttgcaga
     5761 ccaccatgag gggcaccagg ggcatggcga gccccatggc cctaagggtt cccgtgccaa
     5821 tggcgatgac ctgggggtcg ctggcgatga accacgtcat gatcgccggg gccccgaacc
     5881 acccccccag ggcgcacagg accatgaggg cggtggcggt cttgaaggtg aaaagaaacg
     5941 ccccccgcac ccggtcgaag ttccccgccc catagttgta ccccaaaacg ggctgatatc
     6001 cctgtcccac gccgatgacc acggaaaaag ccaccatgaa gactttgccc acgatggaca
     6061 gggccgccac cgccgcgtcg ccgtagacgg cggcgctgag gttcgtggcg atggccgcca
     6121 cgctggccaa cccctggcga cagaaggagg gaaggccgtt tttgacgatc tgcccgtaga
     6181 gagcgggggc cttcgccacg tacctcacat ggagagatgt ctgggtgcga tgggtgagaa
     6241 agccggatag tagcagagag aagctcacgc actggctcag cgctgtggcg atggcggccc
     6301 cggcaatgcc caggcggaag gtgaagatga aaaggggatc taggaagatg ttgaggatcc
     6361 ctccggcggt gatgccgatc atggcgaact gggcccttcc ttccgcccgc aggatgttgt
     6421 tcaggaggaa ggacgccgcc atgatggggg ccgcatagag gatgtaggag gcgtactctt
     6481 tggcgtaggg aaggatggtg tccgtggcgc ccaggaggcg catgagggga tcgatggccc
     6541 cgagcccccc caccgccacc gctcccccga ggagcaggga gaggtagtag gcgctggagg
     6601 ccacgctccg agcttcgtcc aggaggcgcg cccccaggag gcgcgagatc caggtgcccg
     6661 cccccatgcc gatggtgaac cccacggcct gaatgatggc catgagggaa aagaccaccc
     6721 ccaccgcccc cgaggcgctg gtgccgatct gggacacgaa ataggtatca gccatgttgt
     6781 aaatcgacgt caccagcatg ctgatgatcg tcggcaccgc cagggaggag ataagccttc
     6841 ccacaggaac ggtggtcatc ttcaagaact ggctctgggc gtggggcatg ggggcgcccc
     6901 cctttcctgc gtcctggggc cttcccgagg gagccttcgg taccctaggg atccccaagg
     6961 ggggccgggg cccggacggc gaggtttttt ctcttctttc ctcggcaaga gtaccacaaa
     7021 aaccgccggg gccggaccat ccacctgaag agagggcgtg gggccaaagg cttgtttgtt
     7081 gcagaatatg attattacgg tctccgagag ccaccgttcc gatcttgacg gagccacctc
     7141 tccgatgaaa gtgagccacc gttccggcga gatggagccg gcgttccgga gggggagcca
     7201 cccaatgagc gggtaggctc tgtttgttgc agaatagaag tgggcaaagt tgcaagacaa
     7261 aaatccccag atgtgcaggg gtgaaatcct agagaatgca aaggcgccat tgaaagcctg
     7321 accggatcct cggggtaacg tccgggggcc cggagggcct cgacccttta ggggccatgg
     7381 gcgatgcctt gttttggtga cgaaaa
//
LOCUS       DAOOER010000077         7179 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_10513_length_7179_cov_6.528355, whole genome shotgun sequence.
ACCESSION   DAOOER010000077 DAOOER010000000
VERSION     DAOOER010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 7179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7179
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10513_length_7179_cov_6.528355"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="PL162_08375"
     CDS             complement(<1..817)
                     /locus_tag="PL162_08375"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712825.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HQF92071.1"
                     /translation="MHALAGKPAPLDMLVNVPRLVAAYYTRYPDVGDGAQRVSFGTSG
                     HRGSSLRGSFNEAHVLAMAQALAEYRTEEGLRGPLFLGMDTHALSEPALLTALEVFAA
                     AGLEVRYQENFGYTPTPVISHAILTWNRDHPALPGDGVVITPSHNPPEDGGFKYNPPS
                     GGPASPEVTKRIQDRANELLGENLAGVKRLPLKRALCASGVRPHDYVTPYVEDLGHVV
                     DLEAVAAAGLRLGVDPMGGSGIAFWDPLAARWGLDLEVVNRRVDPTFSFMTLDS"
     gene            complement(1005..1556)
                     /locus_tag="PL162_08380"
     CDS             complement(1005..1556)
                     /locus_tag="PL162_08380"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HQF92072.1"
                     /translation="MRQRRPLPAPWSRAAFSVPRRTRRRGGGARPFLGGLLGGVLLAA
                     LAQGFRWGFLTGALSPGVFTGGALRGALVFNKGLAFSLFSEALPGALLAGGGVLVLGC
                     LVAWCGRRLSSGAWAFCWGGALSNAFERWTEGGVLDYLGVCPPWGGAPIFLNVADLFL
                     MVGVLWSLVDLGRWDPPEPDLRP"
     gene            complement(1625..>2361)
                     /locus_tag="PL162_08385"
     CDS             complement(1625..>2361)
                     /locus_tag="PL162_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQF92073.1"
                     /translation="PQVRPESGAIFGVEALVRWRGQDGKLISPGDFIPLAEETGLIVP
                     LGAWVLRTACAQARQWLDAGLGPLEMSVNISAVQLYRSSLWELVAEQMEEFFLPPGTL
                     ALEITENTILQDWERATATFRELKKLGIILYIDDFGTGYSSLSYLRDFAIDGLKIDKS
                     FVDHIPDHGKNEALVRGILAIAQGLGLRAVIEGVETEAQERALRAMGSDLLFQGYRYS
                     PPVPFEKMELLLRRQRLGGSDAAFFA"
     assembly_gap    2362..2461
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2462..4221)
                     /locus_tag="PL162_08390"
     CDS             complement(<2462..4221)
                     /locus_tag="PL162_08390"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQF92074.1"
                     /translation="MAEDDGKVFEPPREGGGLDWEEWAGVPFLVLDDEGRCVRLGGLG
                     SSIYAESDPEGAFVHAVEGAEALRREVREAVRDLRGKEVSFRAGGRRWKGAALPVTLA
                     SGHRGALVVGIRSPAGALPSPSPERDGWSDDRSGSFLSALPDAFFLMNDKGVVEDLRR
                     GRYFFLLVPPEEILGRPLGAIPGLPPGMARRCEAALDAVRRGGGLVRFEYDLEFDGGA
                     RHFEARFFPVGQEKIGLLLRDLTPLQLAKSDLALAEKIIESAQEGIVVTDAAGSIETV
                     NAAFSRITGYSREEALGHNPRILKSHHHDATFYRHMWQALRSVGKWEGEIWNRRKGGE
                     VYPEWLAIHALRNTEGEITHYVSVFSDLSQIKAQEAQIEHQAYHDRLTGLPNRALFLN
                     LLAVEIDHARQSLGTVGMLFLNLDRFKSVNDSLGHVAGDRVLMEMGRRLQGFAGEGRT
                     VSHTGGDEFWLLVPSPKNPKELARIATEVLELLKTPVDAGGFSIYLTASIGIALFPDD
                     GQDTVALTGAADRAMNQAKAGGGDTYSFHTEEMNRSARKRFDLETGLRRAVAREDFCL
                     HYQPQVRPESGAIFGVEALVR"
     gene            4321..5457
                     /locus_tag="PL162_08395"
     CDS             4321..5457
                     /locus_tag="PL162_08395"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQF92075.1"
                     /translation="MHRTSGVVFRLALITFCVFGFANAYFLLASFLELRGFSPATTGL
                     MVSAFYGTTLMTRPLAGSCIERRGTRFTLLAAGGCALLGSGGLMLFSSAPGIFCCRVV
                     SGIGFSLGTVALAAYQGLAIPPEERGKSFALTAIGSIVTMLVVVPLGEGLMGAGYPLG
                     YLALPPLLALGTIALGGSLPPLSASQVPPSWGTWRDLLGLRSYRWILGTALCFSLADA
                     SVLFLSTLVGEAGLSASPFMAATAGGAVLARTLGLRIMTPARRPSLAPAAAALMGLAV
                     GLLPWAHSAALLTLFGILFGVGVGFAYPVNLALVGDLLPPALGPKGSAGLLFAMDLCW
                     MAVPLGLGAATPLMGLSRAYTIFALVIVAGAFLLALPLRGPKRS"
     gene            complement(5454..6911)
                     /locus_tag="PL162_08400"
     CDS             complement(5454..6911)
                     /locus_tag="PL162_08400"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQF92076.1"
                     /translation="MDGTIYGGEGMGSSADIDRTIEHRKRTSEILVVEDSLTQAKRLE
                     RLLKGNGYGVALARNGLEGLDQARKRRPAVVITDVMMPEMDGYEMCRQIKADPGLRHI
                     PVVILTSLSDPRDVIQGLQCGADNFLTKPYDDAHLLGRLYHILANEGLRQAGHAQMSV
                     EVFFAGQFHKLTADRIQIIDLLLSTFEAAVLQSTQLAKLGGDYREALEEIKRTQANFR
                     TLMETTGDAVVVVGADGLVRYVNPATEVLFDCDASAVTGKPFWIPIGGEGSREVSLPS
                     SQGGNAVGDMRVVRSNWDGEGVMLATIRDVTETVRLRERLEVEAVSDALTGLFNRRGF
                     FKIAPGRLSLAERMDLAVVCFFADLDNFKQVNDLLGHEEGDQVLREAAEVLKKTFRES
                     DLLARIGGDEFAVLMVQDRGEKWDGESACAQVKARLERNLELCHGRSSRPYRLAMSVG
                     FAVAQKVQGISLDALVAEADGKMYENKAAKRSCSG"
     gene            complement(7019..7174)
                     /locus_tag="PL162_08405"
     CDS             complement(7019..7174)
                     /locus_tag="PL162_08405"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HQF92077.1"
                     /translation="MTFAQDKESAVVWGMPGEAVRRDGATHVLSPQGVARVLVRMAEG
                     ADRKGAP"
BASE COUNT         1188 a         2517 c         2096 g         1278 t
ORIGIN      
        1 cggagtccaa ggtcatgaag ctaaaggtgg gatccacccg gcggtttacc acctctagat
       61 ccaaccccca gcgtgccgcc aggggatccc agaaggcgat gcccgatcct cccatgggat
      121 ccacccccag gcggagaccc gccgccgcca ccgcttcaag gtccaccacg tggcccagat
      181 cctccacgta gggggtgacg tagtcgtggg ggcgcacgcc ggaagcgcag agcgcccgct
      241 tcaggggaag gcgcttcacc cccgccagat tttctcccaa caactcgttg gcccgatcct
      301 ggatgcgttt tgtcacctcg ggagaagcgg gcccccccga gggggggttg tacttaaagc
      361 ccccgtcctc gggggggttg tgggaggggg tgatgaccac cccatctccg ggaagggcgg
      421 ggtgatcccg gttccaggtg aggatggcgt gagagatcac cggcgtgggg gtgtagccga
      481 agttttcctg atagcgcacc tccagccctg cggcggcgaa gacctccagg gcggtgagca
      541 gggcgggctc ggaaagggca tgggtgtcca tgccgagaaa gagaggaccc cgaagcccct
      601 cttccgtgcg gtactccgcg agggcctggg ccatggccag tacgtgggct tcgttgaagc
      661 tcccccgcaa cgaggatccc cggtgccccg aggtgccaaa agacacccgc tgggccccat
      721 ctcccacgtc ggggtatcgg gtgtagtatg ccgccaccag gcggggaacg ttcaccaaca
      781 tgtcgagggg tgcgggctta cctgccagag cgtgcatctc tctccacctc catttctttt
      841 ttgccacgtc ccatccgttg ccccatttcc cccggcgaag gggggtgccc ccggagagat
      901 cttccgccag gggcatcccc gacgccggga cacgcgcact tcccggattt tttcccgaga
      961 ggccccttgc ggcacgtggc cccttcccgg aagcccccgc ccccttaggg gcggagatcc
     1021 ggttccgggg gatcccatcg ccccaaatcc accaggctcc acagcacgcc gaccatgagg
     1081 aaaagatccg ccacgttgag aaagatcggg gcaccgcccc agggaggaca gacccccaga
     1141 tagtccagca ctcccccttc ggtccaccgc tcgaaggcgt tgctcagggc ccctccccag
     1201 cagaaggccc aggctcccga agagaggcgg cggccgcacc acgccaccag acatcccagc
     1261 acgaggaccc cgccccccgc caggagggcc ccgggaaggg cctcagaaaa gagggaaaag
     1321 gccagccctt tgttgaacac cagagctccc cgcagggccc ccccggtaaa gaccccggga
     1381 gaaagggcgc ccgtgaggaa cccccaccgg aacccttggg ccagagccgc cagaagaact
     1441 ccccccaaaa gcccccccag aaagggcctc gcgccgccgc cccggcgacg ggtcctgcgg
     1501 ggcacggaga acgcggcccg agaccagggg gcggggaggg ggcgccgctg ccgcaagatc
     1561 tcctcggagc gctctcttcg gagggcctcc ggatccagga gaggaggaag gggttccccc
     1621 atggttacgc gaagaacgcc gcatccgagc ccccaaggcg ctggcggcgc aacaggagct
     1681 ccatcttctc gaagggaacg gggggactgt agcggtagcc ctgaaagagg agatcgctcc
     1741 ccatggcccg cagcgcccgt tcctgggctt cggtctccac cccttcgatc acggcacgaa
     1801 ggcccaatcc ctgggcaatg gccagaatac cccgcaccag ggcctcattt ttcccatggt
     1861 caggaatgtg gtccacgaaa gatttgtcta tttttagtcc atcaatggcg aagtctcgaa
     1921 gatagctgag cgaggaatac cccgtgccga aatcatcgat gtagaggata attcctagtt
     1981 ttttcagttc tcggaaggtg gccgtcgccc gctcccaatc ctgcaggatg gtgttttccg
     2041 tgatctccag cgccaaggtc cccgggggta ggaagaactc ttccatctgt tccgccacca
     2101 gctcccacag cgacgagcgg tagagctgca ccgccgatat gttcaccgac atctctaggg
     2161 ggcccagtcc cgcgtcaagc cattggcggg cctgggcaca ggcggtgcgc agcacccagg
     2221 cccccagagg aacgatgagc cccgtctcct ccgccagggg aatgaaatct ccgggagaaa
     2281 tgagcttgcc gtcctgcccc cgccatcgca ccagggcctc cacgccaaag atcgctccgg
     2341 actcgggccg cacctggggt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 ncgcaccagg gcctccacgc caaagatcgc tccggactcg ggccgcacct ggggttggta
     2521 gtgcagacag aaatcctccc gggccaccgc ccggcggagc cccgtctcca ggtcaaagcg
     2581 cttgcgggcc gaccggttca tctcctcggt gtggaaggag tacgtgtctc cccctccggc
     2641 cttggcctgg ttcatggctc ggtccgccgc ccccgtgagg gcgacggtgt cctgcccgtc
     2701 gtcggggaaa agggcgatgc cgatgctggc ggtgaggtag atcgaaaagc cccccgcatc
     2761 cacgggagtt ttcaggagct ccagcacttc ggtggcaata cgggccagct ccttggggtt
     2821 tttgggggag ggcaccaaga gccagaactc gtcccccccc gtgtggctca ccgtcctccc
     2881 ctcccccgca aagccctgga ggcggcgccc catctccatc agcacccgat ctcccgccac
     2941 atgaccgagg ctgtcgttta ccgatttaaa tcggtctaag ttgagaaaga gcatccccac
     3001 cgtgcccagg ctctgccggg cgtggtcaat ttccaccgcc aggaggttta ggaatagggc
     3061 acgattgggc aaccccgtga ggcggtcgtg ataggcctgg tgttcgatct gggcctcctg
     3121 ggccttgatc tgggagaggt cactgaagac cgacacatag tgcgtgatct ctccctccgt
     3181 gttccgcaac gcgtggatgg cgagccattc ggggtagacc tcgccccctt tgcggcggtt
     3241 ccagatctct ccttcccact tacccacgct ccgcagggcc tgccacatgt ggcgatagaa
     3301 ggtcgcgtcg tggtgatggg acttgagaat ccggggattg tgccccagag cttcctcccg
     3361 ggagtacccc gtgatgcggg agaacgcggc gttcaccgtc tcaatgctgc ctgcggcatc
     3421 ggtgaccacg atgccctcct gggcgctctc gatgatcttt tccgccaggg ccaggtcgct
     3481 cttcgccaat tgcaggggcg taagatcccg taaaaggagc cctattttct cctgccccac
     3541 ggggaaaaac cgagcctcga agtggcgcgc tcctccgtca aactccagat cgtattcaaa
     3601 acgtaccaag cctccacctc gccgcaccgc gtcgagggcg gcctcacatc gccgggccat
     3661 ccccggtgga aggccgggga tggcccccag ggggcgcccc aggatctctt ccgggggaac
     3721 caagaggaaa aaatacctcc cccgccgaag gtcttccacc acccccttgt cgttcatgag
     3781 aaaaaaagca tcgggcaggg ccgacaaaaa ggacccgctt cgatcgtcgg accatccgtc
     3841 acgctccggc gatggggaag gcagggctcc ggcggggctc ctgatcccca ccaccaacgc
     3901 tcctcggtgc cccgaagcca aggttacggg gagcgcagcg cccttccacc tgcgccctcc
     3961 cgcccgaaag gacacctcct tcccccggag atcccgcacc gcttcccgta cttcccggcg
     4021 aagggcttcc gccccctcca ccgcgtggac aaaggcccct tcggggtctg attcggcata
     4081 aatcgacgat cccaaacccc ccagacggac acagcgcccc tcgtcgtcca acaccaggaa
     4141 gggaactccc gcccactcct cccaatccag gccccccccc tcccgggggg gctcgaagac
     4201 tttcccgtca tcttcggcca cgatcgttcc cctcccctcg cttccggggg atctctccgc
     4261 ttgaccgccc cctgctgcgg atgtaggctg attccacggc tcatccttgg gagggacccc
     4321 atgcaccgca cgtccggagt ggtctttcga ctcgccctaa taaccttctg cgtctttggc
     4381 ttcgccaacg cgtacttctt gctggcgtct tttttggaac tccggggatt ttctcccgcc
     4441 accacgggcc ttatggtcag cgccttctac ggtaccacct tgatgacccg ccccctggcg
     4501 gggagctgca tcgagcgacg aggcacccgt ttcaccctcc tggcggcagg gggatgcgcc
     4561 ctcttgggga gcggggggtt gatgctcttc tcctccgccc cggggatctt ttgctgtcgg
     4621 gtggtgtcag gaatcggctt cagcctcggc accgtggctc tggcggccta ccaggggctg
     4681 gccatccccc cggaagaacg gggcaagagc ttcgccctca ccgccatcgg atccatcgtc
     4741 accatgctgg tggtggttcc tctgggggag gggctcatgg gcgccggata ccccctggga
     4801 tacctggccc tgccccccct gttggccctg gggaccatcg ccctgggagg aagcctgccc
     4861 cccctgagcg cctcacaagt gcccccctcc tggggcacct ggagggacct cctgggcctt
     4921 cggagctatc gatggatcct ggggacggcc ctgtgcttct ccctggcgga cgcttcagtg
     4981 ctctttcttt cgaccctggt gggcgaggcg gggctctccg cttcgccctt catggccgcc
     5041 accgccgggg gggccgtcct ggcccgcacc ctagggctcc ggatcatgac gcccgccagg
     5101 cgtccctctc tggcgcccgc cgccgccgcc ctcatgggcc tcgctgtggg gctacttccc
     5161 tgggcccatt ccgccgccct gctgacttta ttcggcatcc tcttcggcgt gggggtgggt
     5221 tttgcctacc cggtgaacct cgccctggtg ggagatctcc ttccgccggc cctgggcccc
     5281 aagggctctg cagggcttct tttcgccatg gacctctgtt ggatggcggt gcccctgggg
     5341 ctgggagccg ccacgcccct gatggggctc tcccgggcct acacgatctt cgccctggtc
     5401 atcgtggcgg gggccttcct tctggccctg ccgctccggg gtccgaagcg ctcctaaccg
     5461 gagcacgatc tcttggcggc cttattctcg tacattttcc cgtccgcttc ggcgaccagg
     5521 gcatccaggg agatcccctg aaccttctgg gccacggcaa accccacgct catggccagg
     5581 cgatagggac gggaagaccg cccgtggcag agctccagat tccgctccag gcgcgctttc
     5641 acctgggcgc acgcgctctc cccatcccac ttctcccccc ggtcctgtac catgagcacg
     5701 gcgaactcgt ctccgccgat ccgggccagg aggtcgctct cccggaaggt ctttttcaac
     5761 acctccgccg cctctcggag cacctgatct ccctcttcgt gccccaggag gtcgttcacc
     5821 tgcttgaagt tgtccaggtc cgcaaaaaag cataccaccg ccaagtccat gcgctccgcc
     5881 aggctgaggc gaccgggggc aatcttaaaa aaaccccgcc ggttgaaaag ccccgtgagg
     5941 gcatccgata cggcctcgac ttccaaccgc tcccgcaatc gcacggtttc ggtgacatct
     6001 cggatggtgg ccagcatgac cccttctccg tcccagttgg accgcaccac cctcatgtcc
     6061 cccaccgcgt ttcccccctg ggaagagggt aggctcacct cccgggagcc ctcccctccg
     6121 atggggatcc aaaagggttt tcccgtcacg gcgctggcgt cacagtcgaa aagcacctcc
     6181 gtagcggggt tcacgtagcg caccaggcca tccgccccca ccaccaccac cgcgtccccc
     6241 gtggtttcca tgagggtccg gaagttcgcc tgggtgcgct tgatctcttc cagggcctcc
     6301 cggtagtctc ccccaagttt ggcgagctgg gtgctctgga gcacggcggc ctcgaaggtg
     6361 gaaagcagca ggtcgatgat ctggatgcgg tccgccgtga gcttatgaaa ttgtcccgcg
     6421 aagaacactt ccacggacat ctgggcatgc cccgcctggc gcaggccttc gttggccagg
     6481 atgtgataga gtcgtcccag gagatgggcg tcgtcgtaag gcttggtaag gaagttatcc
     6541 gccccacact ggagcccctg gatcacgtcc cgggggtcgg aaagggaggt taggatgacc
     6601 acgggaatgt gcctaagacc gggatcggcc ttgatctggc ggcacatctc gtaaccgtcc
     6661 atctcaggca tcatgacgtc cgtgatgacc acggcggggc gcctctttcg ggcctggtcc
     6721 aacccctcca agccattccg ggccaaggcc acaccgtagc cattcccttt cagaaggcgt
     6781 tccagtcgct tcgcctgagt aaggctgtcc tccaccacca agatctcgga agtcctcttg
     6841 cgatgctcta tggtcctgtc aatgtccgcc gaagatccca tcccttcccc accgtagatc
     6901 gtgccgtcca ttccgtgtcc caccttttat ctcagcttct tttgtgccat cttcgccgac
     6961 gacgctccgg cggcctcctt cgttccttac cggcaatccg gatcccctat cttctggctt
     7021 aaggggctcc cttccggtcc gctccctccg ccatcctcac caggacccgg gccacgcctt
     7081 ggggggaaag gacatgggtc gccccatccc gccgcaccgc ttctcccggc atgccccaca
     7141 ccacggcgct ttctttgtcc tgggcaaagg tcacgccgc
//
LOCUS       DAOOER010000078         7167 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_10532_length_7167_cov_8.687992, whole genome shotgun sequence.
ACCESSION   DAOOER010000078 DAOOER010000000
VERSION     DAOOER010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 7167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7167
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10532_length_7167_cov_8.687992"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="PL162_08410"
     CDS             complement(<1..782)
                     /locus_tag="PL162_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HQF92078.1"
                     /translation="MLPKEYRTITDLSGPLMMVEKVADVRFDELVEIELASGERRRGR
                     VLEITTDRALVQVFEGTTGIDLNATKVRFLGKVLTLPVAKEMMGRIFNGRGEPIDGGD
                     QILAKKRLDVNGFPINPYSRDFPSEFIQTGISTIDGMNPIVRGQKLPIFSGSGLPHNR
                     IAAQVARQSSVISGHEAFAVVFVAMGITFEEASFFMEDFRRTGAIERTVMFVNLADDP
                     AIERITTPRLGLTCAEYLAFECDMHVLVIMTDMTNYCEALRE"
     gene            complement(772..2565)
                     /locus_tag="PL162_08415"
     CDS             complement(772..2565)
                     /locus_tag="PL162_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HQF92079.1"
                     /translation="MAQHSTVQGTIAKISGPLVVAKGMAGACMFDVVRVGTMGLVGEI
                     IELKGDTASIQVYEETSGVMPGEPVVSTGEPLSVELGPGIIEQFYDGVQRPLKLIEDA
                     AQSPFISRGIAVPAIPRDLAWDFVPRVKKGDEVVPGDILGVVQETVLVEHRIMVPVGV
                     RGKVLKIESGSHLVEDVIAVIQDGNDKKEVRMLQRWPVRTSRPVAKRLPPYVPLTTGQ
                     RVVDLFFPLARGGTACVPGPFGSGKTVIQHQLAKWADAEIVVYVGCGERGNEMTDVLL
                     EFPELEDPRSGHPLMKRTVLIANTSNMPVAAREASIYTAITMAEYFRDMGYSVALMAD
                     STSRWAEALREMSGRLEEMPGEEGYPAYLGTRLASFYERAGRVICLGSQQAEGSITSI
                     GAVSPPGGDLSEPVTQNTLRVTKVFWGLDASLAYQRHFPAINWLTSYSLYAEKLGEYW
                     DTQYDDEWSALRTEAMSLLEDEDRLREIVRLVGIDSLSREERMVLETSKSLREDFLHQ
                     NAFHEVDTFASMDKQFKMLHHILTFHHLAMEALRRGAPLKQILDLPVRDQIARMRYVD
                     EKDLASIDALGETIKKEIGTVMPDGGEVHVA"
     gene            complement(2660..2998)
                     /locus_tag="PL162_08420"
     CDS             complement(2660..2998)
                     /locus_tag="PL162_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HQF92080.1"
                     /translation="MFDTTTSERMAAVGDYESVLPFQAVGVDPYFLGEDASDVELLLR
                     RLSREYAVVFLVESCFEDHRNLIDDLNDEFSVSIIPIPSQTGARGVGVENIRSSVERA
                     VGMDIFSVES"
     gene            complement(2991..4001)
                     /locus_tag="PL162_08425"
     CDS             complement(2991..4001)
                     /locus_tag="PL162_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HQF92081.1"
                     /translation="MPTPERYGYATARIRAMERRFVDQGVLQRMLDAEDLSSALKVLG
                     ETAYAPWLAEMKSERDFDRPISSEMFYCYEEVRQFVPDPEMGQLLRITYDFHNIKVIL
                     KSMLRHRRGQSRRWDLLTNLASRETDDLLAAMESEDYRLLPYGLSGVMGECLSIWEQT
                     QDIVEVEKRLDAQLFRALGELAKATAFPGVLRWFRARVDAENIRNLLRLRRAGEEGVK
                     ISEFLHEGGNVSLERLVDLGSEPVESWSRVLHYADVAPYLAEMGEQGSSEAMLVMLEK
                     ALDDYISGMLAPFSHTPFAPENVLRFLWSKEIETKNVRIVLVSKTSGLDRDMVRKVLR
                     RV"
     gene            complement(4016..4597)
                     /locus_tag="PL162_08430"
     CDS             complement(4016..4597)
                     /locus_tag="PL162_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HQF92082.1"
                     /translation="MALADIKKKIETEARQEAGKLLQRAREEADAILAEADLQAEKMR
                     QSYERRLQEEEPEVLRRRRIVADMDVQRLELGARREVIGKTFETALGALRALPAEKYE
                     AFVTALLENAMITKSEEVLVGRKEKVVTAAWLEKFNKAHDASLTLSSEKLDGSGGFVL
                     SQGRVDVNCTFDMLVHSLTEDLEADVVERLFSS"
     gene            complement(4620..5099)
                     /locus_tag="PL162_08435"
     CDS             complement(4620..5099)
                     /locus_tag="PL162_08435"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HQF92083.1"
                     /translation="MEQITGMMLAVLGAALAAGWAASGSAIGIGIAGESGAGVMTEDP
                     GKFGLVLLLQALPGTQGIYGLLTAFFAIMKVGLLGGEPVAVSLWQGLGILFACLPVAI
                     GEYTSGIAQGKTSAASIQMIAKRPEEMGKAIIMPAMVETYAVLSLLMSIILLNSIKI"
     gene            complement(5118..7139)
                     /locus_tag="PL162_08440"
     CDS             complement(5118..7139)
                     /locus_tag="PL162_08440"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HQF92084.1"
                     /translation="MAVLPVKKVSITVHRSVEEEVFGKVQAFGRCEVVPLVSQGDVSH
                     AAARLAEVDDTLADVRFALRFLEPHFSGGGGGLALLFGDKPQVTLPDLAQMSHNLGLP
                     CIVQDLREKERRLNEIRTEKSLGVSVQNVLQHLEGFPYPLVLLTEGTARVQGIFGTVA
                     CGNMDEAKRLLSSALQPLGEIFPFIPKDPKEKEGHIALIFPREQYRSVEELLAGVGFS
                     RTELPGVLGDTVEEEQRRFSARAVELAAQEEALISATRDLAARWVPDLRHTLDYLSVV
                     RDRRSTLLQGMRTEQVLMISLWCPAPEVTRLQKVLDPFSQEIDVALADPEEGETPPTV
                     FQNSSWAEPFEPLTKLFGVPEYGAIDPTALMAPFFVLFFGMCFGDGGYGLVTGGLAFY
                     LFKKYRMTGDARGYVLMIMAGSVSTILYGALTGSWFGDMIDAFPFLHVFQPLKNSVTI
                     MDPVKDPMTVLKVSLALGVVQIFFGLFVALYDNVRKGSYMAAFSDQGGWLVFLTGLIL
                     LGGGASGALPAEGMAIYQGVAALGALILFLTQGREKEGIFRKALSGALSIYSVSSYLG
                     DVLSYSRLLALGMASAAVGMIVNMLAVLISDVPYVGWLLGAVLFVGGHLFSIAVNVLG
                     AFIHSLRLQYVEFFSKFYGAGGRIFSPLRYDTKFVAIVDKEPEGAQIIS"
BASE COUNT         1506 a         2252 c         1993 g         1416 t
ORIGIN      
        1 atctcccgga gagcttcgca gtagttggtc atgtccgtca tgatgaccag gacgtgcatg
       61 tcgcactcga aggccaaata ctccgcacag gtgagcccca ggcgaggagt ggtgatgcgt
      121 tcgatggcgg ggtcatccgc caggttcacg aacatcaccg tacgctcgat ggctcctgta
      181 cggcggaaat cttccatgaa gaacgacgcc tcttcaaagg tgatacccat ggccacaaag
      241 accacggcaa aggcctcgtg gccgctgatg acggaggact ggcgggccac ctgggccgcg
      301 atccggttgt gaggaaggcc gcttcccgag aagatgggga gcttttgacc ccgcacgatg
      361 gggttcatgc cgtcgatggt ggaaattccc gtctgaataa attcggaggg gaagtcccgg
      421 gaataggggt tgatgggaaa accgttcacg tcgaggcgtt tctttgccaa aatctggtcg
      481 ccgccgtcga tgggttctcc tcggccgtta aaaatccttc ccatcatctc cttggccacg
      541 ggaagggtca gcaccttccc cagaaatcgc accttagtag cgtttaaatc tatccccgtg
      601 gtcccttcaa agacctgcac cagggcccga tcggtggtaa tttccagcac gcgcccccgg
      661 cgccgctcgc cgctcgccag ctcgatctcc accagctcat cgaaccggac gtccgcgact
      721 ttctccacca tcatcagggg gcccgaaagg tcggtgatgg tgcgatactc cttaggcaac
      781 atggacttca cctccatcgg gcatcacggt cccgatttcc ttcttgatgg tctcgcccag
      841 ggcgtcgatg gacgccaagt ccttttcgtc tacgtagcgc atccgggcta tctgatcgcg
      901 cacgggaaga tcaaggattt gtttgagagg cgccccgcga cgaagtgctt ccatggcaag
      961 gtggtggaag gtcaggatgt ggtggagcat cttgaactgc ttgtccatgg atgcaaaggt
     1021 gtccacctca tggaaggcgt tctggtgcaa gaaatcctcc cgaagagact tggaagtctc
     1081 cagcaccatc cgctcttctc gggataagga gtcgatcccc accaggcgca cgatttcccg
     1141 caggcggtcc tcatcttcca acaacgacat ggcctccgtg cgcagggcgc tccactcgtc
     1201 gtcgtactgg gtgtcccaat actctcccag cttttcggcg tagagcgagt agctcgtgag
     1261 ccagtttatg gcggggaagt ggcgctggta cgccagggag gcatccaatc cccagaagac
     1321 cttggtcacc cgcagggtgt tctgggtcac cggctccgaa aggtctcccc ccggaggaga
     1381 tacggcgccg atagaagtga tggacccctc agcttgctgg gatcccaggc agatgacgcg
     1441 cccggcgcgc tcgtagaacg acgccagtcg ggtccccagg tacgccggat acccttcttc
     1501 tccgggcatc tcctcgagac gcccggacat ctcgcggagc gcctcggccc aacggctggt
     1561 ggagtccgcc atgagagcca cggaatatcc catgtcccgg aagtactccg ccatggtgat
     1621 ggcggtatag atcgaggctt cccgggcggc cacgggcatg ttggaggtgt tggcgatgag
     1681 cacggtccgc ttcatcagag gatgccccga ccggggatct tcgagttcgg ggaattccag
     1741 aaggacgtcc gtcatctcgt tgccccgctc tccgcacccc acgtatacca cgatctccgc
     1801 atccgcccac ttggcgagct ggtgctggat cacggtcttg ccagagccga aggggccggg
     1861 aacacaggcg gtcccccctc gcgccagggg aaagaagagg tccaccaccc gctgtcccgt
     1921 ggtgagcggc acgtaagggg ggaggcgctt cgccacaggg cgagaggtcc gcacgggcca
     1981 tcgctgcagc atccgtactt cctttttatc gttcccatcc tggatgacag cgataacgtc
     2041 ttcaaccaag tgggatccgg attcgatctt gagcactttc ccccgcacac ccacggggac
     2101 catgatccgg tgttccacca acaccgtctc ttgcaccacc cccaaaatgt ctcctgggac
     2161 tacctcatca ccttttttta ctctaggcac gaaatcccag gccaggtccc gggggatagc
     2221 gggcacggca atgcctcggg agataaaagg actttgcgcc gcgtcttcga taagtttcag
     2281 gggacgctgc acgccgtcgt agaactgctc aatgataccc gggccgagtt ccacggaaag
     2341 aggctccccc gtgctaacca caggctctcc cggcatgacg ccggaggttt cttcgtacac
     2401 ctgaatggat gcggtatctc ccttgagctc aatgatctct cccacgagcc ccatggtgcc
     2461 gacccgaacc acgtcgaaca tacacgctcc agccatgccc ttcgccacca cgaggggccc
     2521 ggaaatttta gcaatggttc cttgaaccgt actatgctgg gccactacct atcgcctcca
     2581 tcgggggcac agcaccctgt gcccggggtt ccgacatcct cttcttgtga taaatccttg
     2641 agaatccgcc atggggcttt taggactcca cggaaaaaat atccatgccc acggcccgct
     2701 ccacgctgct tcggatgttc tccaccccca caccccgggc cccggtttga ctggggatgg
     2761 gaatgatgct tacggagaat tcatcgttaa gatcgtcgat cagattccgg tgatcttcaa
     2821 agcagctttc caccagaaat accactgcgt attccctcga aagacgccgg agaaggagct
     2881 ccacgtccga ggcgtcttct ccaagaaaat agggatccac gcccacggcc tgaaagggca
     2941 gcacgctctc gtaatctccc accgccgcca tgcgttccga agtcgtcgta tcaaacacgg
     3001 cgcaacacct tccttaccat atcccggtcc aggccgctgg tcttggaaac caaaacaatg
     3061 cgaacatttt tcgtctctat ttcttttgac cagaggaacc gaagcacgtt ttccggcgca
     3121 aagggggtgt gggaaaaggg ggccagcatg ccggaaatat agtcgtccaa ggccttttct
     3181 agcatcacca gcatggcttc ggaacttccc tgctccccca tttccgccag gtagggggcc
     3241 acatccgcat agtgcaacac ccgagaccag gattcgacag gttcgctccc cagatccacc
     3301 aaacgctcca gggacacatt tcctccttcg tgcaggaact cggatatttt taccccttct
     3361 tctccggcgc gccttaacct cagcaggttt cgaatgtttt ccgcatccac ccgggctcga
     3421 aaccaccgaa ggacccctgg gaacgctgtg gccttcgcca gctcccccag ggcccgaaat
     3481 agctgggcat ccaggcgctt ttccacttca acgatgtctt gggtctgttc ccagatggag
     3541 aggcactccc ccatgacgcc ggagaggccg tagggcaaca accgatagtc ctcgctttcc
     3601 atggcggcga ggaggtcatc ggtttcccga ctggccaagt tcgtcagcag atcccaccga
     3661 cgggactgcc ctcggcgatg gcggagcata ctctttaata ttacttttat gttgtgaaaa
     3721 tcgtaggtga tcctcagaag ctgccccatc tcggggtcag ggacaaactg cctcacttcc
     3781 tcgtagcagt agaacatctc cgaggagata gggcgatcga agtcccgctc acttttcatt
     3841 tcggccaacc agggagcgta cgccgtctct cctaggactt taagggcaga agagagatcc
     3901 tccgcgtcca gcatgcgctg caggacgccc tggtccacga aacggcgctc catcgcccga
     3961 atgcgggcgg tggcgtagcc gtaccgctca ggcgtgggca ttcccccacc tccccttagg
     4021 aggagaacag gcgttcaacc acgtctgcct ccaggtcttc tgtaagggag tgtaccagca
     4081 tgtcgaaggt acagttcacg tccacgcgtc cctgggaaag aacgaatccc ccagatccgt
     4141 ctagcttttc tgaagacaac gtcagggaag cgtcgtgggc cttgttgaac ttctccagcc
     4201 atgccgccgt gactaccttc tccttacgcc ccaccagcac ttcttcgctt ttggtgatca
     4261 tagcgttttc caggagcgcc gtcacgaagg cttcgtattt ctccgctgga agggcccgta
     4321 gcgcccccag ggccgtctca aaggttttgc cgatcacttc gcgccgcgcc cccagctcca
     4381 ggcgctggac atccatgtct gccacgatgc gacggcggcg caacacttcg ggctcctctt
     4441 cctgtaagcg gcgttcgtaa gattggcgca tcttctccgc ttggaggtcc gcctcggcaa
     4501 gaatggcatc ggcttcttcg cgagcgcgtt gaagaagctt gcccgcttct tgacgggcct
     4561 ccgtttctat cttttttttg atatccgcga gggccatgtg ggcatccgcc ttcctagcac
     4621 tagatcttga tgctgttcaa gaggatgatg ctcatgagca gtgagagcac cgcataggtt
     4681 tccaccatgg cgggcatgat gatggccttt cccatttcct cgggacgctt ggcgatcatc
     4741 tggatggagg ccgccgaggt cttaccctgg gcgatccccg aggtgtactc gccgatggca
     4801 acggggaggc aggcaaagag gatgcccaac ccctgccaca gggagacggc cacgggctca
     4861 ccgccgagaa gccccacctt catgatggca aaaaacgccg tgagcaggcc atagatacct
     4921 tgggtaccgg gaagagcttg gaggagcagg acgaggccaa acttccccgg atcttccgtc
     4981 atgactcctg cgcccgactc tcccgcgatg ccgataccga tggcggagcc agaagccgcc
     5041 catcccgccg ccagggctgc cccaaggact gccagcatca ttccagtgat ttgctccatt
     5101 ttcacaacac ccctctttta ggatattatt tgtgcccctt cgggctcttt gtctacgatg
     5161 gcaacaaact tggtgtcgta tcgcagggga gagaaaatac gcccccccgc gccgtagaac
     5221 ttgctgaaaa actccacgta ctgaagacga agggagtgaa taaaggcccc caggacgttt
     5281 accgcaatgc tgaaaagatg tccaccgaca aaaagcaccg ccccgaggag ccatccgacg
     5341 tagggtacgt ccgagatcag cacggccagc atgttgacga tcatgcccac ggcggcggag
     5401 gccatgccca gggcaaggag ccggctgtag cttaacacgt ctcccaggta ggaagagacg
     5461 ctgtagatac tcagcgcccc ggagagggcc ttgcggaaaa tcccctcctt ttctcttcct
     5521 tgggtcagaa agaggatgag ggcccccagg gccgccacgc cctgatagat cgccatgcct
     5581 tctgcgggaa gggccccgga ggcgccgccc cccagcagga tgagccccgt gagaaagacc
     5641 agccaaccgc cctgatcgga aaatgccgcc atgtagcttc ccttgcgcac gttgtcatag
     5701 agggccacga agagcccaaa aaatatttgc accactccca gggccaacga caccttgagc
     5761 accgtcatgg ggtctttcac cgggtccatg atcgtcacgc tgtttttaag gggctggaag
     5821 acgtgcagaa aaggaaaggc gtcgatcatg tcgccgaacc aggagccggt gagcgccccg
     5881 tagaggatgg tggaaacgct ccccgccatg atcatcagta cgtacccccg ggcatccccc
     5941 gtcatacgat actttttgaa caggtaaaac gctaatcctc cagtgacgag tccatagcct
     6001 ccgtcgccga agcacatgcc gaaaaagagc acgaaaaacg gagccatcaa ggccgtgggg
     6061 tcgatggcac cgtactccgg aacgccaaag agcttggtga gcggttcgaa aggctctgcc
     6121 cacgaggagt tctggaacac cgtggggggg gtttctccct cctcggggtc cgccagggcc
     6181 acgtctattt cctgggaaaa ggggtcaagg accttctgaa gccgagtcac ctctggagca
     6241 ggacaccaca gagagatcat gagcacctgt tccgtcctca tcccctggag aagggtggat
     6301 cgccggtccc ggaccaccga aaggtagtcc agggtgtgcc gaaggtctgg aacccaccgg
     6361 gcggccaaat cccgagtggc cgagatcagg gcctcttcct gggccgcgag ttccactgcc
     6421 cgggccgaaa atcgacgttg ctcttcctcc acggtatccc ccaggacccc cggcagttcc
     6481 gtacgagaaa aacccacccc cgccaggagc tcttccaccg agcgatattg ttcccggggg
     6541 aaaatcaacg cgatgtgccc ctctttttcc ttaggatcct tggggatgaa ggggaaaatc
     6601 tctcccagag gctgcaaggc cgacgagaga aggcgcttcg cctcgtccat gttgccacaa
     6661 gccacggtac caaagattcc ttgcacccgg gcagtcccct ccgtcaggag aaccaatgga
     6721 taggggaatc cttccaggtg ttggaggacg ttttgcaccg aaactcccag ggacttctcc
     6781 gtacggattt cgttaaggcg gcgctctttt tctcttaagt cttgcacgat gcagggaagg
     6841 cccaggttat gggacatctg agccagatcc gggagagtca cctgaggctt gtccccgaag
     6901 agtaacgcca atcccccgcc ccctccggaa aaatggggct ccaggaagcg cagggcaaag
     6961 cgcacgtcag ccagggtgtc atccacctcg gcgagacgcg ccgccgcgtg ggagacgtcc
     7021 ccttgggaca ccaaaggaac cacctcacag cgtccaaagg cctggacctt gccaaaaact
     7081 tcctcttcca cggaacggtg gacggtgata ctaaccttct ttacgggcag aacggccata
     7141 acgcgccatc acctcttccg cgatcca
//
LOCUS       DAOOER010000079         7114 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_10634_length_7114_cov_5.668225, whole genome shotgun sequence.
ACCESSION   DAOOER010000079 DAOOER010000000
VERSION     DAOOER010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 7114)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7114)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7114
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10634_length_7114_cov_5.668225"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2247)
                     /locus_tag="PL162_08445"
     CDS             complement(<1..2247)
                     /locus_tag="PL162_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92085.1"
                     /translation="MKKPGDMVRRALGVALLVGCLAVPGAWGEGRLFSVGPLGVEIPS
                     GWFPFPEGDGGWGFFTGGLPEGGEPFVSVMFRLPPDDGEFQEFRKQMEGSRRDPQASV
                     DIVEAPGMVGPWPGTLLRFSVTMKEGKMKGKNFQGSLFFSQNGVSGDRRLMVLTGGDP
                     ALAREREGDVAALLGSLREGGASYVLSPDLLLERSPGEFGGAAALAVDGQGLIYVADW
                     RQRIIRVLDAPGTLRDTWGKQGKGEPGTFAQIKALWGLPEGGVMVGDGGYGYDAPVLH
                     ECDATGNFRRRVELKGKADPPMEYILSLSRNGAEELLVADGAISEGNRLSVLTSEGAL
                     TRSWSVPKLQGAAWEPSGGVVAITRSEDRMGLMVRFSPEGEPREVWNAFGRGTPPPGW
                     EAVYFDPKSVAVDPCGRIAVLDTWNEAVWIYDPDGTLKDRLDLKLLARAGILEGLASD
                     PQGNIFVLDRGSAGSVPPRVLVIRRTEPCELPTGSPERGPDALPSPLPDPTPTLSGEG
                     APELSPTGDLLTDRLGAWKKALELRRQAADLLREGRRDEAIALYEKSLGLYEDAALRA
                     YLEDLRRGAPLATPAPASGDRGDDQEDEQMEDLPPLESLAEEVPETPELDLPEPEISG
                     DLVKLPELPPVATLAPSRAPTAVPEPSPTPEPELPDLPPMLTLAPTREPGAVTLSPTP
                     EPELPPSPYEGEWITLMRAEGAPGGAPPGFALLTVRRQGDMLRCTLHDRQVFLLRDEG
                     PERAEGSLG"
     gene            complement(2307..5561)
                     /locus_tag="PL162_08450"
     CDS             complement(2307..5561)
                     /locus_tag="PL162_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92086.1"
                     /translation="MRYLSVVMIALFLWQSGALALPPPSDAALEQEVERLSQALALRE
                     EGETLLRGGDKAGALERFRRSLALFPDRALELRIQKLAEELDAGGPGAGTSPPPMPTP
                     PTQPTQPPMPPRPTRGEVVLQKTLSQDGGTLVVAGNLSIALDPGALSGEVPFSLRPAR
                     VAPLPLVGGSSLPLEGVWDVDAGGASFSSPARISLDLPREARRDDVLLVRPVFSRSGV
                     NWTPLPYQRRGDTLLFEAPHFSFTGMVLIALLAPLIPVAFVYFHGGEQWPDSTLFNAE
                     APFVADLIPDPAGFHIAWTHLLGDGKDGVRDPAGLAAAEQAAKDRFIASQSAIFQDRD
                     RALASPKLPEVERLKALMEADRRMDGARSDLEKDLAKAKEDYLVPEEVKLIREGLVFG
                     RSYLLSRGFSSPDVLDVFVPRHCGKDDGTLNNRYWGRNYMMVGASLDRDDIFSTTMHE
                     QFHHFQGQYLKAGKANIYLAEASAALMEREAADHYRQHAGVTIFSDNRFLNGLKEGLN
                     GKPIKTYEGDAGPFQRLGYGLSWFLEYLRDQWAKQPGNLSEDFHKKFLVQCQALGGSP
                     TPVEAFSWGAGGSRSALGKVFASFVREAVLEGLPSHTLYEKKYGIPLFDGTLGDVHDP
                     LSFDAQAVQEITDAMVKPWSLQFFRLAPPKTLSGEPMPRLVLFFPAEWFDASSTAGRQ
                     IFLRMRSGDGALSLDTPPGKDYAGVVSFDATRYLYVVDTGQTGSGYWYDYKPGMLVCL
                     EAPQKLTASLRNKTLEIVWQEPRLAKERPELVTGYRIQVRRLTEKIPRVDRTVPRGSP
                     RLTLGEADGVGSFENLEVSARTVMTLGDRRDESEPAMALATPESSKIFPLFENRTYWT
                     TATPLGNDPGVVFPPVPLAVSVTLGGVTGSMGPEAPEWKAYLKLAENVAGTDAFLKKK
                     FEAELASKGITLTEEQMRQALQSMQVAGVFMPEESSQKRTLLGGGWRAPGASVAVTLQ
                     VTLPPVPRIPLSLPGPDGNPRPLEAQVEVSRWFMMVGPQKSPEFPLGSGTLSWTPKSA
                     SEDAFGIDLALPFSLTFFDPATGEVLKEGGVPLKYENFNAIFPLGVFFCPEKKP"
     gene            5790..>7114
                     /locus_tag="PL162_08455"
     CDS             5790..>7114
                     /locus_tag="PL162_08455"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQF92087.1"
                     /translation="MKNSARIKGKIYVFIFLTIFVALLLSLSLSYYSVCRVQREFFRA
                     QMRQTLEDRGAFLEEWLARRVAVLEMARAFPDRFERGGFFSPDRLLRMGFSDIYRGWE
                     SGAFQSFAGWVPPPDYRPASRPWYRQTRSADALTASDPYLDPISGLMTITLGIPIESP
                     EDGPGVMAGDIQMGDDHAWLKNIEVGEVGFLWLINDRGVVLQHPRGEMRFKVLRDFPE
                     MASLDRILFSAPEGEFEYTRRGDHRRAIFRTIRPFGWVLGVTLREDEALGAFRQFRDV
                     HLALMVILLTLLGGVAWLVARSLGAPLARIITFVEEVASGNLEATLKPSHHREIDSLT
                     TSLRKMGDRLREDFAVIGAQKEALALINQELESRVQERTEELERANEELRTSRDAMEI
                     LASTDYLTGLLNRRAFWERASQKMDQSPRGEGGIAVFMADLDGFKAVNDR"
BASE COUNT         1212 a         2276 c         2362 g         1264 t
ORIGIN      
        1 ccccagggag ccctcggcgc gctccggccc ctcgtcccgc agcagaaaga cctgccggtc
       61 gtgcagggtg caccggagca tgtccccctg gcgccgcacc gtcagaaggg cgaatcccgg
      121 gggcgcccct cctggagccc cttcggccct catgagggtg atccattccc cttcgtaggg
      181 agacggggga agttcgggtt ccggcgtggg ggagagggtc accgcaccgg gttccctggt
      241 gggggcgagg gtgagcatgg ggggtagatc ggggagctcg ggctccgggg tgggtgaagg
      301 ctccggcacc gccgtcggcg ccctggaggg ggccagggtg gccacggggg gcagctcggg
      361 aagctttacc aggtcgcccg agatctcggg ctcggggagg tctagctcgg gagtctcggg
      421 gacctcttcg gccaggctct ccaggggagg aaggtcttcc atttgctcgt cttcttgatc
      481 gtcgcccctg tcgccagagg ccggggccgg ggtcgccaag ggggcgcccc gccgcaaatc
      541 ctccagatag gcccgcaggg cggcgtcctc gtaaagtccg aggctcttct cgtacagggc
      601 gatggcctcg tcccggcgcc cttcccgcag gagatccgcc gcctggcgcc gcaattccag
      661 ggcttttttc caggctccaa ggcgatcggt gagcagatcg ccggtggggg agagctccgg
      721 cgccccttcc ccggacaggg tgggggtggg gtcggggagg ggagagggga gggcgtccgg
      781 cccacgttcc ggcgaccccg tcgggagttc gcagggctcg gtgcgtcgga tcaccagcac
      841 ccggggggga acgctcccgg cgcttccccg gtcgagcaca aagatatttc cctgaggatc
      901 cgatgccaga ccttccaaaa tgcccgcccg ggccaaaagt ttcagatcaa gtcggtcctt
      961 cagggtgccg tcggggtcat agatccacac cgcttcgttc caggtgtcca ggacggcgat
     1021 gcggccgcag gggtccaccg cgacggattt cgggtcgaag tacaccgctt cccatcccgg
     1081 aggtggggtg ccccggccga aggcgttcca gacctcccgg ggctctccct cgggagaaaa
     1141 gcgcaccatg agccccatgc ggtcctccga acgggtgatg gccaccacgc ctccggaggg
     1201 ttcccaggcc gccccctgga gcttgggcac gctccaggag cgggtgaggg ccccctcgga
     1261 ggtcagcacg gagaggcggt tcccttcact gatggcccca tcggccacca gaagctcctc
     1321 cgcaccgttt cgggacaggg aaaggatgta ttccatgggg ggatcggcct tgcccttgag
     1381 ctcgacgcgc cgccggaagt tccccgtcgc atcgcactcg tgcagcacgg gggcgtcgta
     1441 gccgtagcct ccgtcgccca ccatcacgcc tccctccggg agcccccata gggccttgat
     1501 ctgggcgaag gtccccggtt cccctttgcc ctgcttgccc caggtgtccc ggagggtccc
     1561 cggggcgtca agcactcgga tgatccgttg gcgccagtcg gcgacgtaga tgagcccttg
     1621 gccgtccacc gccagggcgg cggcgccgcc gaattccccc ggagaccttt ccaggagcag
     1681 atcgggggag aggacgtacg aggctccccc ttcccgaaga gagcccagga gggccgccac
     1741 gtccccttcc cgctcccggg caagggcggg gtctccccca gtcagcacca tgaggcgccg
     1801 gtctccgctc actccgttct gggaaaagaa caggcttccc tggaagttct ttcccttcat
     1861 tttcccttct ttcatggtca cggaaaagcg caggagcgtg ccgggccagg gccccaccat
     1921 ccccggggct tcgacgatgt ccaccgaggc ctggggatcc cggcgggagc cttccatctg
     1981 ctttcgaaac tcttggaatt ctccgtcatc cgggggcaga cgaaacatga cggagacaaa
     2041 gggctcgccg ccctcgggaa gaccccccgt gaaaaagccc caccccccgt ccccttcggg
     2101 gaacggaaac catcccgaag gaatttccac ccccaggggg cccacggaga aaagccgtcc
     2161 ctcgccccag gcccccggca ccgccaggca tcccaccagg agcgccactc ccagcgcccg
     2221 ccgcaccatg tcacccggtt ttttcatagg tatccctccc gttttttcct gcggtcgttc
     2281 cgatcttgac gcgacgatgc ccatggtcag ggctttttct cggggcagaa gaacaccccc
     2341 aggggaaaga tggcgttgaa gttttcgtat ttcaggggga ctcccccttc tttgaggact
     2401 tcccccgtgg cggggtcgaa aaaggtcaac gagaacggca gggcgagatc gatgccgaag
     2461 gcgtcttcgg aggcgctctt gggagtccag gagagcgttc cgctccccag ggggaactcg
     2521 gggctcttct gcgggcccac catcatgaac catcgagaaa cttccacctg ggcttccaga
     2581 ggacgggggt ttccatcggg tcccggtaac gacaggggaa tacggggcac cggaggcagg
     2641 gtcacctgga gcgtcacggc cacggaggct ccgggggctc gccaccctcc tcccaaaagg
     2701 gtccgtttct gggacgactc ttcgggcatg aagacccccg ccacctgcat ggattgaagg
     2761 gcttggcgca tctgctcctc cgtgagggtg atgcccttgg aggcgagctc ggcttcaaac
     2821 ttcttcttga gaaaggcgtc cgtgcccgcc acgttttcgg cgagcttcag ataggccttc
     2881 cactccggcg cctcggggcc catggacccc gtcacccctc ccagagtcac cgacaccgcc
     2941 aggggaaccg ggggaaagac cacgccgggg tcgttcccca agggagtggc ggtggtccag
     3001 tacgtgcggt tctcaaaaag gggaaagatc ttggaggact ccggggtggc cagggccatg
     3061 gcgggctcgc tctcgtcgcg gcgatctccc aaggtcatca ccgtgcgggc cgagacctcc
     3121 agattttcaa agcttcccac gccgtcggct tctcccagcg tgagccgggg gcttccccgg
     3181 ggcacggtac gatccacccg ggggatcttt tccgtaaggc gccgcacctg gatgcggtat
     3241 cccgtcacca gttcgggcct ttccttggcc agccggggtt cctgccagac aatttccagg
     3301 gtcttgttcc ggagcgaggc ggtgagcttc tggggggcct cgaggcacac cagcatcccg
     3361 ggcttgtagt cgtaccagta ccccgaaccg gtctgtcccg tgtccaccac gtagagatag
     3421 cgcgtcgcgt cgaaagacac caccccggcg tagtccttgc ccggcggagt gtccagcgag
     3481 agcgccccat cccccgaccg catccgcagg aaaatctggc gtccagcggt ggaggaggca
     3541 tcgaaccact ccgcaggaaa gaagaggacc agccggggca tgggttcccc cgaaagggtc
     3601 ttggggggag ctaggcggaa aaattggagg ctccagggtt tcaccatggc gtcggtgatc
     3661 tcctgcaccg cctgggcgtc gaaggagaga gggtcgtgca catctcccag ggtgccgtcg
     3721 aagaggggga tgccgtactt cttctcgtag agggtgtggg acggcaggcc ctccagcacc
     3781 gcttcccgga caaaggaggc aaagaccttc cccagggcag aacggcttcc ccccgcgccc
     3841 caggagaagg cctccaccgg cgtggggctt ccccccaggg cctggcactg gaccagaaac
     3901 tttttgtgaa aatcctccga aaggtttccc ggctgcttcg cccactgatc ccggaggtac
     3961 tccagaaacc aggaaagacc gtaccccagg cgctgaaagg gccccgcgtc cccttcatag
     4021 gtcttgatgg gcttgccgtt caacccctcc ttgaggccgt tgagaaaacg gttgtcgctg
     4081 aagatggtga ccccggcgtg ctgacggtag tgatccgccg cctcccgctc catgagggcc
     4141 gccgaggctt cggcaaggta gatgttcgcc tttcccgcct tgaggtactg cccctggaag
     4201 tggtggaact gttcgtgcat cgtggtggag aagatgtcgt cccggtccag acttgccccc
     4261 accatcatgt agttccgtcc ccagtaccgg ttgttgaggg tgccgtcgtc cttgccgcag
     4321 tggcgcggca cgaagacatc cagcacgtcc ggcgaggaaa atccccggga gagcagatag
     4381 gagcggccaa aaacgagccc ttcccggatg agcttcacct cctcggggac gagataatcc
     4441 tctttggcct tggccaagtc tttttccaga tcacttcggg ccccatccat ccgacgatcg
     4501 gcctccatca gggccttgag ccgctcgacc tcgggcagct ttggcgaggc cagggctcgg
     4561 tcccgatcct ggaaaatggc gctctgggag gcgatgaagc gatccttggc ggcctgttcc
     4621 gccgccgcga gccctgcggg gtcccggacg ccgtcttttc cgtcccccag gaggtgggtc
     4681 caggcgatgt ggaaccccgc agggtcgggg atcaggtccg ccacgaaggg cgcttcggcg
     4741 ttgaacaggg tggaatcggg ccactgttcc ccgccgtgaa agtacacgaa ggccacgggg
     4801 atgagggggg cgaggagggc gatgagcacc atgcccgtaa agctgaagtg gggcgcttca
     4861 aagagcaggg tgtctccccg gcgttggtag ggcaaagggg tccagttgac cccgctccgg
     4921 gagaagaccg gccggaccaa cagcacatca tcccttcggg cttcccgggg aaggtccaaa
     4981 gaaattcgcg ccggagagga gaaggaggcc ccccctgcgt ccacgtccca gaccccttcc
     5041 aggggcaacg aggagccccc gacgaggggc aggggggcga ctcgggcggg gcggagggag
     5101 aaggggactt ctcccgaaag ggccccagga tccagggcga tggaaaggtt tccggccacc
     5161 accaacgtgc ccccatcctg ggaaagggtt ttttgcagga ccacctctcc ccgggtgggc
     5221 cggggaggca tggggggctg ggtaggctgg gtgggcgggg tgggcatggg agggggcgat
     5281 gtcccggctc ccgggcctcc ggcgtcgagc tcttccgcca gcttctggat ccgaagctcc
     5341 aaagccctgt cgggaaagag ggccagggac cggcgaaagc gctccagggc cccggctttg
     5401 tctccgcctc gtagcagggt ttctccttct tctcgaaggg ccagggcctg gcttaggcgc
     5461 tccacttctt gctccaaagc ggcatcggag gggggaggaa gggccagggc gccgctttgc
     5521 cacaggaaca aagctatcat gacaacagat agatatcgca tcattttccc tcgccgcgcc
     5581 cgagccatga tcatcccccc cgaaaataac cttttctctc tggcatttta ccacactccc
     5641 ccgggtcctt cgcgaaggga aagagatggg ctataatggt cgtcggtgtc ggtcgaaggg
     5701 atgccttgcc tcctccgggg ccttcgcggg gccttgatcc tccctgggca tttcgggggc
     5761 accccatctt ctgacggggg gaagcggtgg tgaaaaactc tgctcgtatc aagggaaaaa
     5821 tctatgtgtt catttttctt acgatcttcg tggccctcct cctttcgctc tccctttcct
     5881 actactccgt gtgtcgggtt caaagggagt tcttccgggc ccagatgcgg cagactctgg
     5941 aggatcgggg ggcctttctg gaggaatggc ttgcgcggcg cgtggccgtg ttggagatgg
     6001 cccgggcctt tcccgaccga ttcgaaaggg gagggttttt tagcccggac cgattgctcc
     6061 gcatgggctt ttccgacata taccgagggt gggagagcgg ggcctttcag agcttcgccg
     6121 gatgggttcc ccccccggat taccgcccgg cgtcgcgccc atggtaccgc cagacccgca
     6181 gcgccgatgc cctgacggcc tccgacccct atctggaccc catctccggc ctgatgacca
     6241 tcaccctggg gatccccatc gaatcccccg aggatgggcc gggggtgatg gccggggaca
     6301 tccagatggg cgatgaccac gcgtggctta aaaatattga ggttggcgag gtgggctttc
     6361 tctggctcat caacgaccgg ggcgtggtgc ttcagcaccc ccggggcgag atgcgattta
     6421 aagtcctgcg ggacttcccc gaaatggcat ccttggacag gatccttttt agcgcccccg
     6481 aaggggagtt tgaatacacc cggcgggggg atcatcgtcg ggcgattttc cgcacgattc
     6541 gcccctttgg atgggtcctg ggggtgaccc tgcgggaaga cgaggccctt ggcgcctttc
     6601 gacagtttcg cgacgtgcac ctggcgctga tggtgatcct gctgaccctt ttgggagggg
     6661 tggcatggct ggtggcccga tccctcgggg ctcccctggc gcggatcatc accttcgtgg
     6721 aggaggtggc ctcgggaaat ctcgaggcca ctttgaagcc ctctcaccac cgggagatcg
     6781 attctttgac gacgtcgctc cgcaagatgg gggacaggct ccgggaggac ttcgccgtca
     6841 tcggcgccca gaaggaggcc ctggcgctga tcaaccagga gctggagtcc cgggtacagg
     6901 agcgcaccga agagctggag agggccaacg aggagcttcg aacttctcgg gacgccatgg
     6961 aaatattggc ctccacggat tacctcacgg gccttttaaa ccggcgggcc ttttgggaga
     7021 gggcgtccca gaagatggac caaagccccc ggggcgaagg ggggatcgcg gtcttcatgg
     7081 cggacctgga tgggttcaaa gcggtcaacg atcg
//
LOCUS       DAOOER010000080         6802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_11191_length_6802_cov_8.279235, whole genome shotgun sequence.
ACCESSION   DAOOER010000080 DAOOER010000000
VERSION     DAOOER010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 6802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6802
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11191_length_6802_cov_8.279235"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..1019
                     /gene="ftsY"
                     /locus_tag="PL162_08460"
     CDS             39..1019
                     /gene="ftsY"
                     /locus_tag="PL162_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HQF92088.1"
                     /translation="MALWTTLKEKLKGVTERWGESIASLFSGKALDEAFWDELEERLL
                     AGDVGIDTTTSLLEEMRRGAMERRIGDTGELRGAFTELLISRLQKIPGMGQPLPSRGN
                     PSVVLLVGVNGNGKTTTAGKLAALASAEGRRVILAGADTYRAAAGDQIRIWGERAGVR
                     TVIQTMGSDAAAVVYDAIQAARSSGADLVIADTAGRLHTKHNLMEELRKVQRIALREA
                     GEDVTETFLVLDAVTGQNALRQAEVFHASLSLTGIVLTKYDSTAKGGILLGVAERFQL
                     PIRYVGLGEGIEDLQLFDPQAFVRTLFSDKDHDQKPSLPGDPSPSLRGRP"
     gene            1016..1969
                     /locus_tag="PL162_08465"
     CDS             1016..1969
                     /locus_tag="PL162_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92089.1"
                     /translation="MTRPPELSSDLSTDNTLQEGLRALFLLGADGIQLKGEDGSRIVL
                     AKEHLIVLLERGLGDETLGALARHPEWCRPYRESRGALSPEMRIIQLEPEGRPEGDAL
                     TELEKFHGDGEEVPCWWSIPLPLLAQRKDRVMLNRHGQGLFPEASPTLGDVHRALARD
                     HLMSWPDQEGKSAKTFLLVPLTEEVFLLEDVSSDMALAEDVAWWAAVGKALLAYNEAR
                     GYRIRLWRGVASPPEFLSNEEAISCQWEGQFLGSLVLEPPSKPVRRRTPKPSPPPSEA
                     QEESPSSAAPSKKKGSTPSKEKKGTSSKGAEEGPLEDGRRG"
     gene            2055..2537
                     /locus_tag="PL162_08470"
     CDS             2055..2537
                     /locus_tag="PL162_08470"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HQF92090.1"
                     /translation="MSHSLWDWTLEQMAAAWGPTERVSEAYPFDMTPYYKNIAETLLR
                     RFVSFVGLFPAENLAEWKRMGGAMEARSGPSRRINVDPGFIDGAKLVLASTKDRAQRV
                     PIAPDLYAEVTLRFRNHRWEPFDYTFPDFRSGRYDDFLSDVRRDWLRDTRDTRRKNHD
                     "
     gene            2530..3837
                     /gene="purB"
                     /locus_tag="PL162_08475"
     CDS             2530..3837
                     /gene="purB"
                     /locus_tag="PL162_08475"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HQF92091.1"
                     /translation="MIDRYATPEMAALWSEEHRFQTWLEVELAACQAWAEEGRIPFEA
                     LEDIRRRASFDVERIREIEETTQHDVIAFVSCVAERTGECGRYIHLGLTSSDVIDTAS
                     ALQLRSSLDVILSSLETLKEAVLSQAWRHKDTLCVGRTHGIHAEPTSFGLKLLNWYDQ
                     LRRDTRRLEAAREEIAVGKISGAVGTYALCPPSIEGRVCDLLSLAVAPVSNQIIQRDS
                     HAFVIGSLALLGTTVERMATEIRHLQRTEVREAWEPFRKGQKGSSAMPHKKNPILSER
                     LCGMARLLRGYAATSLENVALWHERDISHSSAERILWPDAFHLAHYMLSKAQGVIREL
                     PVDPQRMEQNLELTRGLVFSQRVLLALVEEGGMSREEAYRHVQGAALRCWQGEGDFKT
                     LLAQDPPISAALPGDHLDRLFDAGFYLRHVDTIFQRFPRAKVD"
     gene            4281..5516
                     /locus_tag="PL162_08480"
     CDS             4281..5516
                     /locus_tag="PL162_08480"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HQF92092.1"
                     /translation="MGILISSSSRKASFRFLITGITINILAIAYFKYFNFLMQTVSSI
                     FSFPWIDRDIFLPLGISFFTFQQIAYLIDAYQNRTTAKSLLEYTLFVTFFPQLIAGPI
                     VHHDDLLPQFRLKETFQLHWHSLAQGIALLAFGLFKKVVLADSFSPWVGATFDASGPL
                     SALEAWSGALAYTFQIYFDFSGYSDMALGLGKLFRIRLPENFASPYQALSIGEFWRRW
                     HMTLSRFLKDYLYIPLGGNRRGPFRRYLNLLVTMVLGGLWHGAAWTFVLWGTLHGFFL
                     VAYHLWEALRARYGLRPLPLGVGWSLTFFAVVMGWVIFRAPSLERAGDLFQGMMGFHG
                     MGAVADALARGTMPGGLGEVGALAGALGFCLFFPRSTVWCDRHLATPSWRTGALVAGA
                     ALGALLSLTEVSEFLYFQF"
     gene            5539..6609
                     /locus_tag="PL162_08485"
     CDS             5539..6609
                     /locus_tag="PL162_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92093.1"
                     /translation="MNFPSWKKWVLSVLGGVSALQILVVALNVAVDPLWCFGFYSSWN
                     DLSEGFDERQQKTNYVNFLPFSHDTILLGSSRVTYISPPEVPGRSFFNYAVSSMKPRE
                     YAPFVDFAQSRQGRPFRLIILGVDFFGTNGRLRSLDAFAPPKTYIEHAQSPFSRWSTA
                     TSFGALSHSLVDLALNAGWKNPDGDYRRRALSRVFLIPDQGEKRRVVEEQRAYYKEHI
                     YGDYFYDSPYGDYSSLLKNRSSTEFKVFTTPESAVLLQALLEEGRWEDYARWMRELVE
                     AFGAVWNFMYVNRITADLGNYKDAHHFSPQVGEYIMQRLLGIPYEGPEDFGLLVDASN
                     LEEHLAWVRQNIGELQKASVDP"
BASE COUNT         1380 a         2027 c         1951 g         1444 t
ORIGIN      
        1 tttttcgatg aaaagatagc cctaaggagg agatgcccat ggctctctgg accacgctaa
       61 aggaaaaact gaagggcgtc accgaacggt ggggggagag tatcgcctcg cttttttcgg
      121 gaaaagctct ggacgaggcc ttctgggacg agctggaaga aaggctcctc gcgggggacg
      181 tgggcataga caccaccaca tccctgctgg aagagatgcg acggggcgcc atggagcggc
      241 gcatcggcga cacgggagag cttcgaggag cctttacgga gctgttgata tctcgactgc
      301 aaaagatccc cggcatgggg caacccctcc cttcccgggg caacccctcc gtggtgcttc
      361 tggtaggcgt caacgggaac gggaaaacca ccacggcggg gaagctagct gccctggcct
      421 cggccgaagg acggcgcgtc attctagctg gggcagatac ctatcgggca gcggcggggg
      481 accagatccg catctgggga gaacgcgccg gagtgcgcac cgtcatccag accatgggga
      541 gcgacgccgc cgccgtcgtc tacgacgcca tccaagccgc ccgctcctcg ggggccgacc
      601 tggtgatagc agacacggcg ggacgcctac acaccaagca caatctcatg gaagagctcc
      661 ggaaagtcca gcgtatcgcg ttgcgggaag cgggagagga cgtcacagag acgtttttgg
      721 tactggatgc ggtcacggga caaaatgccc ttcgtcaggc cgaagtcttt catgcttccc
      781 tatcgctgac agggattgtg ctcaccaagt acgacagcac cgccaagggg ggcattttgc
      841 tgggagtggc tgaacgattc cagctcccca tacgctatgt gggactcgga gagggaatcg
      901 aggaccttca gctttttgac ccccaagcct ttgtacgaac ccttttttcg gataaggacc
      961 acgatcagaa accttcgttg cccggggacc catctccctc gctcagggga cggccatgac
     1021 gcgccctccg gaactgtcct cggacctttc cacggacaac acccttcagg aagggctccg
     1081 agccctattc ctcctggggg ccgatgggat tcagctcaag ggagaagatg gatcgaggat
     1141 tgttctcgca aaagaacacc tcattgtcct cctcgaaaga gggttgggag atgaaaccct
     1201 gggagctctg gcgcgacacc cggagtggtg tcgcccctac agggaatcac gaggggccct
     1261 ttccccggaa atgaggatca tccagctgga acccgaggga aggccggagg gcgatgccct
     1321 cacggaattg gaaaaattcc acggcgatgg agaagaagtt ccctgctggt ggtccattcc
     1381 cctgcccctt ctggcgcaac ggaaggaccg ggtgatgctc aaccggcacg gccaaggtct
     1441 ttttcccgag gcctctccca ccctaggcga cgtgcatcgg gccctcgccc gggaccatct
     1501 catgtcctgg ccggatcagg aggggaaaag cgccaagacc tttctgttgg tgcccctcac
     1561 ggaggaagtt ttcctcttgg aggacgtgag ctccgacatg gccctagccg aggacgtggc
     1621 atggtgggcc gccgtcggca aagccctttt ggcctacaac gaagcccgag gatatcggat
     1681 tcgcctttgg agaggcgtgg cttcgccgcc ggagttcttg tccaatgaag aagccatctc
     1741 ctgtcagtgg gagggacaat tcttgggaag cttggtgctg gagcccccct ccaagcccgt
     1801 ccggcgccgc actccgaagc cctccccacc cccctcggag gcccaagaag aaagcccttc
     1861 ttcggccgct ccctccaaaa aaaagggatc gaccccttcg aaagaaaaaa aagggacctc
     1921 gtccaaggga gcggaggaag ggccccttga agatgggaga aggggttagt cccatgggcg
     1981 tgggagtgaa gctcgtggtc ggggtgcttt ttcccccctc agagggagct aaaggggctc
     2041 caccggtcgc aggaatgtcc cattccctct gggattggac cttggagcag atggctgcgg
     2101 cctggggtcc tactgaaagg gtcagcgagg catatccttt tgacatgacg ccctattata
     2161 aaaacatcgc cgaaacccta ctccgccgct tcgtctcctt cgtcgggctc tttcctgcgg
     2221 aaaacttggc ggagtggaag aggatgggcg gtgcgatgga ggcccggagc ggtccttcgc
     2281 gccgtatcaa tgtggacccc ggctttatcg atggggccaa gctcgtcctg gcctccacaa
     2341 aggaccgggc ccagcgagtt cccattgccc cagacctcta cgccgaagtc accctacgct
     2401 ttcggaacca ccgatgggag cccttcgatt atactttccc ggatttccga agtggccgat
     2461 atgatgattt ccttagcgat gtgcgccgag actggcttcg tgacacccga gacacaagga
     2521 ggaaaaatca tgattgatcg ttacgccacc ccggagatgg ccgccctctg gtccgaggag
     2581 caccgtttcc aaacctggct cgaggtagag ctcgcggcgt gtcaggcttg ggccgaagaa
     2641 ggacgcatac ctttcgaggc tctggaagac atccgtcgcc gggcctcatt tgacgtggag
     2701 cgcatccggg agatcgaaga gaccactcaa catgatgtca ttgccttcgt ctcctgcgtg
     2761 gcggaacgca cgggcgaatg cggccgctac atccacctgg gtcttaccag cagcgatgtc
     2821 atcgacaccg cctcggcctt gcagctccgc tcctccctgg acgtcatcct ctcctctctg
     2881 gaaaccttga aggaggccgt cttatcccaa gcgtggcgcc acaaggacac cctctgcgtg
     2941 ggacgaaccc atggcatcca cgccgagccc acctccttcg ggctcaaact tttaaactgg
     3001 tatgaccagc ttcgtcgaga cacccgacgc ctggaggccg cgcgggagga gatcgccgtg
     3061 ggcaagatct ccggagcggt ggggacctat gccctgtgcc ccccgtctat tgaagggcgg
     3121 gtctgcgatc ttctgtccct ggcggtggct cctgtgtcta atcagatcat ccagagggat
     3181 tcccatgcct tcgtcatcgg atccctggcc cttctgggca ccaccgtgga gcgaatggcc
     3241 acggaaattc gccacctgca gcggacggag gtgcgagagg cctgggagcc cttccggaag
     3301 gggcagaagg gctcatcggc catgccccac aaaaaaaatc ccatattgag cgaacggctc
     3361 tgcggcatgg ctcgtctcct ccggggctat gccgccacct ccttggaaaa tgtggccctg
     3421 tggcatgagc gagatataag ccattcttcc gccgagcgaa tcctttggcc cgacgccttt
     3481 catctcgccc attacatgct cagcaaggcc cagggggtaa tccgggagct ccccgtggat
     3541 ccccagcgca tggagcaaaa cctcgagctt acccgggggc tggtgttcag ccaaagggtg
     3601 ctcctggccc tggtggaaga ggggggcatg agcagggaag aggcctatcg ccacgtgcaa
     3661 ggggcggccc tgcgatgttg gcaaggggag ggagacttta aaacccttct ggcgcaagac
     3721 ccccccatca gtgcagccct gccgggagac cacctggacc gcctcttcga cgcgggtttt
     3781 tacctccggc atgtggatac gattttccag cgctttcccc gggccaaagt cgactaaaac
     3841 cctccggcgc ccctgacccg tggtaatccg tggagaaggc gtccgagata gggagcgatg
     3901 cccgttgccc ccccggggac ccgtcatggc cgatgaggac ctccccgggg ggatttcccc
     3961 acgccaaaat gggagtttca ccactagaca gccctagggg gcaaggaaaa accgaggaaa
     4021 agaccaggaa gaaccaagga agaaccgagg aagatcgacc caggatgaga ggacggagta
     4081 tccggtgaca acgaatgctt tttaattcac aaatcttcct ctttgtattt ctaccaatta
     4141 cttggtgggc cttctttgta ttgtcttatc aacaaaaaaa agcccttgct cttctgaccg
     4201 tagcgtcagt tatcttttac agttactgga atcccccctt cgttattctt atattattgt
     4261 caatactatt taatttttgc atgggaatcc taatatcatc ttcttctcgc aaagcatctt
     4321 ttaggttttt gatcactggt ataacaatta atatcttagc tatcgcatat tttaagtatt
     4381 ttaattttct tatgcaaacc gtgtcatcta tcttcagttt tccttggatt gatagggata
     4441 tctttctccc cctgggaatt tcgtttttta cgtttcaaca aatcgcctac ttaatagatg
     4501 cctaccaaaa ccgaaccacc gcgaagagtc ttttggaata cacacttttc gtgacgttct
     4561 ttcctcagct gatcgccggg cccatcgtgc atcacgacga tctgttgcct caatttcgct
     4621 taaaagagac ctttcagctt cactggcact ccttggccca ggggatcgcc ctactggcct
     4681 ttggcctgtt caagaaagtg gtcctggcag attccttctc tccttgggtg ggggccactt
     4741 tcgacgcctc cggtcccctt tccgccctgg aggcctggag tggggccctg gcctacacgt
     4801 tccagatcta cttcgacttc agcggatact ccgacatggc cctggggctc ggcaagctat
     4861 ttcgcatccg cctgccggag aacttcgcat cgccctacca ggccctttcc atcggggagt
     4921 tctggcgccg atggcacatg accctttccc gtttcctgaa agattatctg tacatccccc
     4981 tggggggaaa tcgacggggc cccttccgga ggtacctgaa cctcctggtc accatggttc
     5041 tgggggggct ctggcacggg gccgcctgga cctttgttct ctggggaacc cttcacggtt
     5101 tttttctcgt cgcctatcac ctctgggaag ccctgcgcgc ccgctacggc cttcgcccct
     5161 tgcccctcgg ggtagggtgg agcctcacgt tcttcgccgt agtaatggga tgggtcatat
     5221 ttcgggcccc ttccttggag cgcgctgggg acctttttca gggtatgatg ggattccacg
     5281 gcatgggggc ggtcgccgat gcgctagctc gagggacgat gcccggaggc ctgggagagg
     5341 tgggggccct ggcgggggcc ctggggttct gtcttttctt cccccgcagc accgtttggt
     5401 gcgaccggca cctcgcgacc ccctcctggc gcacgggggc tctggtcgcg ggggcggccc
     5461 tcggggccct actgtccctg acagaagtgt cggagttttt atattttcag ttctagagag
     5521 gaagagcgag gaaaaatcat gaattttcca tcctggaaga aatgggtgct ctccgtcctg
     5581 ggaggggtca gcgctctcca gatccttgtc gtagccctaa acgtggccgt ggatcccctt
     5641 tggtgtttcg gattttattc gtcctggaac gacttgagcg aggggttcga cgagcgccaa
     5701 cagaagacta attacgtgaa tttcttgccc ttttcccacg acacgatcct gttggggagc
     5761 agccgggtga cttatatctc tccccccgag gtgcccggtc gaagtttttt taactatgcc
     5821 gtgagcagca tgaaaccccg agagtacgcc cctttcgtcg attttgccca atctcgacag
     5881 gggcgcccct tccgcctcat catcttgggg gttgattttt tcggcaccaa cggaaggctg
     5941 cgatccctgg acgcctttgc cccccctaaa acctacatag aacacgccca gagtcccttt
     6001 tcccggtgga gcaccgccac tagtttcggt gccctgagcc acagcttagt agacctggct
     6061 cttaacgcgg gatggaaaaa ccccgacgga gactaccgtc gtcgcgctct ttccagggtt
     6121 tttctgatcc cagaccaagg ggaaaagcga cgagtggtcg aggaacagcg ggcatactac
     6181 aaagagcaca tctacgggga ctacttttac gattctccct atggggatta ctcttctctt
     6241 ttaaaaaatc gatcgtccac agaattcaag gtcttcacga cgccggaatc ggcggtgttg
     6301 ctccaggcgc tccttgagga gggaagatgg gaggactacg cccgatggat gagagagctc
     6361 gtcgaggcct tcggcgccgt atggaacttc atgtatgtca accgtatcac cgcagacctg
     6421 ggaaactaca aggacgcgca ccatttttcc ccccaggtgg gggagtacat catgcaacgc
     6481 ctcttgggaa tcccctacga aggtccggaa gatttcggcc ttttggtcga tgcctccaac
     6541 ctggaagagc acctcgcctg ggtacggcag aacatcggcg aactccagaa ggcttcggtc
     6601 gatccctgag gaaaaaagaa tagagtcttc actcccttcg agcggcggcc acggccacct
     6661 ggcccagggc aatcccttcg tcattggggg acaccaaccg gggcacccag acgtcgtatc
     6721 cccggcgctc cccacgtttc accgtctcct ccaggaggaa tcggttctgc caaactcccc
     6781 ccgataggac caccggcatg gc
//
LOCUS       DAOOER010000081         6576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_11610_length_6576_cov_6.405919, whole genome shotgun sequence.
ACCESSION   DAOOER010000081 DAOOER010000000
VERSION     DAOOER010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 6576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6576
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11610_length_6576_cov_6.405919"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="PL162_08490"
     CDS             <1..226
                     /locus_tag="PL162_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92094.1"
                     /translation="EVSGMEEAVQEAFSRALPGDTVLLSPACTSWDAYDNYGARGDHF
                     VALVKKEMERRQKCGCEASPATDGSSRPGS"
     gene            248..1393
                     /locus_tag="PL162_08495"
     CDS             248..1393
                     /locus_tag="PL162_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HQF92095.1"
                     /translation="MSEGPKIHRVTEPLLWLIPLLLSALGTVMIASTTSGLSLGEGGS
                     PYAMGMKQLRSLLVGLGGMVGGYAIPTSQWRRWSGGLWILAWGVTAATLLPYFGVSAG
                     GAQRWVQLGPLRFQASDLLSLAAVLHVARKISEEEVPRRKVFFRVSLIAILSMVPLLL
                     QPNMGSALLVLALVMGMYVERHGWRYPLMGGVMGVLPLALLVVWQPYRMRRFWGWLNP
                     WSDPLNTGFQIIQSLVAFANGGVWGLGVGHGLQKLQYLPESHTDFIFSAIGEELGLVG
                     TLGVLGLFIMWAWRARAVYLPLRGTFEGTLVWGLALTVLLPVVINVGGVTKIFLLTGL
                     PLSFVSYGGSSLVMMWVRVGMLLRLTRDSREGRRLPGWTTEGKKALP"
     gene            1390..2472
                     /locus_tag="PL162_08500"
     CDS             1390..2472
                     /locus_tag="PL162_08500"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HQF92096.1"
                     /translation="MTPLIWLVAGGTGGHLWPAVAFGQWLQSRGEGWTPMILAGSRSL
                     EREILETTTLPWESLPLEGSPLGVSGTARWGRWKGLWQSWARCRELLRRHRPWAAVLF
                     GGYVSLPALGACMAHRVPVVFHEQNARAGRATRMAAALGVPVASGWDECDPLKPGAFR
                     PVGIPLRVMERLPNRAEWNKLSDGPPIPERRHVLVLGGSLGSGFLEKRVRDLARRHPF
                     RRWTFLLLGGKGGGDMPENCLAVPRQWDMGPLYAGADLALSRGGGATLAELLSLGLPS
                     LVIPWQGASEGHQAANAAAFVRLGGGMVLPESAPLESLEEALKSLSRMKRRQITVAGP
                     CASEKLWREVMAVATMKGDAFRNGRG"
     assembly_gap    2539..2638
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2639..3733
                     /gene="murC"
                     /locus_tag="PL162_08505"
     CDS             <2639..3733
                     /gene="murC"
                     /locus_tag="PL162_08505"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HQF92097.1"
                     /translation="RGIGIAGTHGKTTTSSMISLVLDAAGRSPTVAIGGELCDIGGNA
                     KLGEGPEMVAELDESDGSFELFNCDVAVVTNVDWDHVDHYPDVESVRTAFVRFLNRVK
                     AGGTVVTCAEDLGALAVAERSVPERLITYGWGKSWEWGATEVQHIPGGGVSFSVLHRG
                     VPMGNLSLRLSGDHNVLNALAACAVADLMGIPFDVVRSALGSFRGAKRRMQWVGTTND
                     IIIYDDYGHHPREVEVTLRAVRQIFPGRRLLAIFQPHRYTRTGALYKDFSRVLSLADE
                     SFLLPIYPADEEPLEGVSSSLIAEAMAMGGAPCHLCFNMKDAQQKVLSHALPGDVILT
                     VGAGNVCLLGGLLLHALREIPREHAVVVGA"
     gene            3711..>4227
                     /locus_tag="PL162_08510"
     CDS             3711..>4227
                     /locus_tag="PL162_08510"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HQF92098.1"
                     /translation="MRWLSALKRRLACSLRERVPLASMTTWGVEGRARYFASPARDGD
                     LSAIMEVWEHESFPLWILGGGSNVLPLGGELPGLTVRVERSQELSFSSDSRGGVVVDA
                     PGGMFLPFLLGVLARRGWSGCEPLAGIPGTLGGAVVGNAGGSDGAIGDVVAWVDTVEP
                     GGIRRRWEEGDL"
     assembly_gap    4228..4327
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4389..5192
                     /locus_tag="PL162_08515"
     CDS             4389..5192
                     /locus_tag="PL162_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92099.1"
                     /translation="MRRKKRWGYIKACALCMALLGGGALAAEDFFQWSRLRTFVVHPE
                     GVIAEEVLWSLVPRGALRCWWIFPESFRTVEDYLDRMYPVRANLDLVGFGDVVCSVVP
                     LHPVLVVRWRGEDWYLDDEEGAIWRTDLPENLQVQGLQRPEGPPLEWGEDMLVPVAPA
                     GEDRRVFRSRIPSGLLRAWHKALDTLKIRSRVERWRVRKCKGLEFVEITLPMGGHSVT
                     VVLDGTNAAAWVQMVPAVEKIVGTLGGGTATLVADTTYGDKIVVRKNYQ"
     gene            5250..6554
                     /gene="ftsA"
                     /locus_tag="PL162_08520"
     CDS             5250..6554
                     /gene="ftsA"
                     /locus_tag="PL162_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HQF92100.1"
                     /translation="MNAEPELLVGLDIGASKVAVVVAERDDVSGEAQIIGIGQSASQG
                     IRKGLVVNLEQAVQSVRRAVDDARNIVGLPIEEVSVAFGGSDVTSIRSRGMISLGRTS
                     RQVTVEDIGRVIEAAQTEIVPALNQTIVHTIPIEYAIDGHSGIDDPLGMTGVRLEMEL
                     QSLIAPTSVVQNVLTCVERAGLRVRCIIIKPLASALGVLTAEEAGAGAVVIDVGAGTT
                     GVAVYAEGKPKRLAVIPVGGDHVTNDLACVLRIPMSKADILKREVSLDEEGDHLEDEL
                     EFQNRGKTLVCTVREVVDVISSRLEELFGALVESEVAESGVSVLPAGIVLTGGVANTL
                     GVERLVSHLMDMPVRIGRPLDVHRMPPERNGVEYASAAGIIRYVVERERNPQKYLDPF
                     QGAGEPRRKVVHSVPDSKDPWIKGNPFAGAWTAIKKTLKELF"
BASE COUNT         1120 a         1613 c         2270 g         1373 t
ORIGIN      
        1 agaggtctcc ggcatggaag aggctgtcca ggaggccttt tcccgggccc ttcccggaga
       61 tacggtgctt ctttctccgg cctgtaccag ttgggatgcc tacgacaact acggagcccg
      121 aggggatcac ttcgttgcgc tggtcaaaaa agaaatggag cgtcgtcaga agtgcggctg
      181 cgaagcatcg ccagcgacgg atggaagctc tcgccccggt tcttagggct gtctcgaggg
      241 atctctcgtg agcgaaggcc cgaagatcca tagggtgacg gaacccctat tatggctcat
      301 acctctcctg ctcagcgcgc tggggaccgt gatgatcgcg tccaccacca gcggcctttc
      361 cctgggagag ggaggatctc cttacgccat gggcatgaag caacttcgga gtcttttggt
      421 ggggttagga gggatggtag gaggatatgc cattcccacg tcccagtggc gccggtggag
      481 tggggggctt tggattctgg cgtggggcgt gacggcggct accctcctgc cgtatttcgg
      541 cgtaagcgcg gggggggccc agcgctgggt ccagttgggt cccttacgtt ttcaagcttc
      601 ggaccttttg tctctggcgg cagtgctaca cgtggcgaga aaaatttctg aagaggaggt
      661 gccccggaga aaagtttttt ttagggtctc attgattgcc attctctcca tggttccttt
      721 gctgttgcag cccaacatgg ggagcgcgct gctggtcctc gcgctggtca tggggatgta
      781 cgtggagagg cacggatgga gatacccctt gatggggggg gtgatggggg ttttgcccct
      841 ggccctcctg gtggtgtggc agccgtatcg gatgcgccgg ttctggggtt ggttaaatcc
      901 ttggtccgat cccttgaata cgggtttcca gattatccaa agcctggtgg cctttgccaa
      961 cggaggggtt tggggattgg gggtaggaca tgggctccag aagctccagt atcttccaga
     1021 atcccacaca gattttattt tctctgccat cggcgaagag cttggcttgg tggggacgct
     1081 gggggtcttg gggcttttca tcatgtgggc ctggcgtgcc cgggcggtgt atttgcccct
     1141 gaggggcact ttcgagggca ccctcgtgtg ggggttggcg ctcacggtcc ttttgcccgt
     1201 ggtaatcaac gtgggagggg ttacaaagat tttcctcttg acggggctgc ctttgtcatt
     1261 cgtgagctac gggggaagtt cgttggtcat gatgtgggtc cgggtcggta tgctacttcg
     1321 ccttactcga gactcccggg agggacgccg tcttccgggg tggacgaccg aggggaaaaa
     1381 ggcgctgccg tgaccccgtt gatctggttg gtggctggag ggacgggggg acatctttgg
     1441 cctgcggtgg ccttcggcca gtggctccag tcccggggcg aggggtggac tcccatgata
     1501 ttggcgggaa gccgctcttt ggagcgcgaa atcctcgaaa cgaccactct tccctgggaa
     1561 agcctccctc tggagggctc tcccctggga gtgtcgggga cggcgcgctg ggggcgatgg
     1621 aaagggcttt ggcagtcttg ggctcgctgc cgagagcttc ttcgtcgtca ccgtccctgg
     1681 gcggcagtgc tgtttggggg gtacgtctcc ctccccgcgc tgggggcttg tatggcacat
     1741 cgtgttcccg tggtctttca cgagcaaaac gctcgagcgg gaagggcgac gagaatggcg
     1801 gcggccttgg gggtgcccgt ggcttcgggg tgggatgaat gtgatcccct gaaacccggg
     1861 gcgttccgcc ctgtgggaat tccccttcga gtcatggaaa gattgccaaa tagggccgaa
     1921 tggaataaac tgtccgatgg cccgccgatc cctgagaggc gtcacgttct ggttttgggt
     1981 gggtcgctcg gaagcggctt cttggaaaaa cgagtgcgag atttggcccg acggcatccc
     2041 tttcggcggt ggacgtttct cctcctggga ggaaaagggg gaggagacat gccggaaaac
     2101 tgcttggcgg tgcctcgcca gtgggatatg gggccccttt atgcgggggc cgatctggcc
     2161 ctatcccgag gaggaggggc caccttggcg gagctcttgt ctctgggatt accatccctc
     2221 gtgatcccgt ggcaaggggc ctccgagggg caccaggcgg caaacgctgc ggccttcgtc
     2281 aggttgggag gggggatggt gttgccggaa tccgcccctc tggaatcctt ggaagaggcc
     2341 ttgaagtcgt tgtcacgaat gaaacgtcgg caaatcactg tcgccgggcc ttgcgccagc
     2401 gaaaaactgt ggcgagaagt gatggcggtg gcgaccatga agggagatgc ctttcgcaat
     2461 ggacgggggt aacgtctcat tggagaatgt gagtaaggtc caccttgtgg ggattggcgg
     2521 ggcggggatg agtgccctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncg
     2641 gggcattggc attgcgggta cccatgggaa aacgaccacc tcctctatga tttccctcgt
     2701 cttggatgcc gctggtcgat ctcccaccgt ggccattggg ggagagctct gcgacatcgg
     2761 aggcaacgcc aagctgggcg aagggcctga gatggtggcc gagttggacg aaagcgacgg
     2821 atccttcgaa ctttttaact gcgacgtggc ggtggtcacc aacgtggact gggatcatgt
     2881 ggaccactac cccgacgtgg agagcgtgag gacggccttt gtcaggttct taaaccgggt
     2941 gaaagccgga ggtacggtcg tgacctgcgc cgaggatctg ggggccctgg cggtggcgga
     3001 gcggagtgtc cccgaacgcc tgataaccta cgggtggggg aaaagctggg agtggggggc
     3061 cacggaagtc caacatattc ccgggggggg cgtatctttt tccgtacttc accggggagt
     3121 gccgatggga aatttgtcgc tgagactttc cggcgatcac aacgtgttga atgcgttagc
     3181 ggcctgtgcg gtggccgatt tgatgggcat accctttgat gtggtccgca gtgccttggg
     3241 ttcttttagg ggagcgaagc ggcgcatgca gtgggtgggg accactaacg acataataat
     3301 ttacgatgat tatggccatc accccaggga agtggaagtc accctgcggg cagtgcggca
     3361 aatttttccc ggacggagac tgctggccat cttccaacca caccgctaca cccggaccgg
     3421 cgcgctgtac aaggactttt cccgggtcct ttctctggcg gacgaatcct ttctgcttcc
     3481 catctatcct gcggacgaag aacctctgga aggggtgtct tcatcgctca ttgccgaggc
     3541 gatggccatg ggaggggcgc cctgtcatct ttgctttaac atgaaagacg cccagcaaaa
     3601 agtgctttcc cacgccttac ccggggacgt gatcctcacc gtgggggcgg gaaacgtgtg
     3661 tctcttaggg gggctcttgc ttcacgccct tcgcgagatc ccaagagaac atgcggtggt
     3721 tgtcggcgct taagcgtcga ctggcctgtt ccttgcggga acgggtgccc ctcgcttcca
     3781 tgaccacgtg gggggtggag gggcgggccc gatattttgc ttcccctgcc cgagatggag
     3841 atttgagtgc catcatggag gtgtgggagc acgaatcctt tccactgtgg atcctggggg
     3901 gggggagcaa cgtcctgccc ctggggggcg agttgcccgg cctgacggtc cgcgtggaga
     3961 gatctcagga attatctttc tcctccgact cccgaggggg cgtcgttgtg gacgccccgg
     4021 ggggcatgtt tctgcccttt cttttgggag tactggcgcg gcgggggtgg agcggttgtg
     4081 agcccctggc aggcatcccc ggaaccctgg ggggagcggt cgtggggaat gcggggggaa
     4141 gcgatggagc catcggggac gtggtggcct gggtggacac ggtggaacca gggggaatcc
     4201 ggcggcgttg ggaagagggc gatctgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnncgg aggcgggtca aggaaaccta tggcgttcta ttagagctcg aagttcaggt
     4381 atggggcgat gcgccggaag aagcgttggg gatatattaa ggcttgtgcc ctgtgcatgg
     4441 cgctcttggg cgggggagcc ttggctgcgg aggacttctt ccagtggagc cgattgcgga
     4501 cttttgtggt ccaccccgag ggagtcatcg ccgaagaggt gctctggagt ctggtgcccc
     4561 ggggggcttt gcgttgttgg tggattttcc cggaatcttt ccgaacggtt gaagattacc
     4621 tcgataggat gtatcctgta agggctaacc ttgacctggt gggatttggc gatgtggtgt
     4681 gttctgtggt acccttacat ccggttctgg tggtgagatg gcggggggaa gattggtacc
     4741 tggacgacga agaaggggcc atatggcgca ccgacctccc ggagaacctc caggttcaag
     4801 gcctccagag gcccgagggg ccccctttgg aatgggggga ggatatgctt gtccctgtgg
     4861 ctcctgccgg ggaagatcgc cgggtctttc ggtcgcgcat tccctcaggg ttgttgcggg
     4921 cgtggcataa agccctggat accttgaaga tccgctcgcg agtggagcgt tggcgggttc
     4981 ggaaatgtaa ggggctggaa ttcgtggaaa tcacccttcc tatggggggg cactcggtga
     5041 cagtagtcct ggacgggact aatgcggcgg cctgggtcca aatggtaccc gcagtggaga
     5101 agatcgtggg aacccttggc ggcggcaccg cgacccttgt ggcggacact acttatgggg
     5161 acaagatcgt ggtccgaaag aactatcagt agggccttgg gatggggcgt tggaagatct
     5221 ggctgtgaac ggaagggagc gggcatgtcg tgaacgcaga accggagctc cttgtagggt
     5281 tggacatagg cgccagcaag gtggcggtgg tggtggcgga gcgtgatgac gtgtccggcg
     5341 aggcccaaat tattggcata ggccaatcgg cttcgcaagg catccggaag ggccttgtgg
     5401 tgaacttgga gcaggcggtt cagtccgtgc gccgggccgt ggacgacgca cgaaacatcg
     5461 tgggactgcc catagaagag gtttccgtgg cctttggcgg gagcgatgtg accagcatcc
     5521 ggtcccgggg catgatatct ctggggcgga cctctcggca ggtgaccgtg gaagacattg
     5581 gccgggtcat cgaagcggcc caaacggaaa tcgttcccgc cctgaaccag accatagtcc
     5641 acaccattcc cattgagtac gccattgatg ggcatagcgg catcgacgac cccttgggga
     5701 tgacgggggt gcgcttggag atggagctcc agtcccttat tgcccccaca tcggtggtcc
     5761 agaatgtcct cacctgtgtg gagcgggcgg gattgcgggt gcgatgcatc atcatcaaac
     5821 ccctggccag cgccctaggg gtgctgacgg cggaagaggc gggagcgggg gcggtggtga
     5881 tcgacgtggg ggcaggaacg acgggggtgg ccgtctacgc cgaagggaag cccaaacgcc
     5941 tcgcggtaat cccggtgggg ggggatcatg tgaccaatga cttggcatgt gttctccgca
     6001 ttcccatgag caaagccgac atcctgaagc gagaggtgtc cctggatgaa gagggagatc
     6061 atctggagga cgagttggag tttcaaaacc ggggcaagac cttagtttgc acggtgcgcg
     6121 aggtggtgga cgtgatttcc agccgcctgg aagagctctt cggcgccctg gtggagtcgg
     6181 aagtggcgga gtcgggcgtt tccgtgttgc ctgcggggat cgtcctcacg gggggcgtgg
     6241 ccaacacgct gggcgtcgaa cggctggtgt cgcatctcat ggatatgccg gtgaggatcg
     6301 ggcgtcccct tgatgtgcac cgtatgcctc cggagcgaaa cggtgtagaa tatgcctcgg
     6361 cggcggggat cattcgctac gtggtggaaa gggagcgcaa cccgcaaaag tacctcgatc
     6421 ccttccaagg ggcgggggag ccacggcgta aggtcgttca ttccgtgccg gattccaagg
     6481 atccatggat caagggtaat cccttcgcgg gggcgtggac tgctattaaa aaaacgctaa
     6541 aagagctttt ctgatttcct tcgagcgccg gacact
//
LOCUS       DAOOER010000082         6401 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_11972_length_6401_cov_6.644028, whole genome shotgun sequence.
ACCESSION   DAOOER010000082 DAOOER010000000
VERSION     DAOOER010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11972_length_6401_cov_6.644028"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /locus_tag="PL162_08525"
     CDS             complement(<1..229)
                     /locus_tag="PL162_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HQF92101.1"
                     /translation="MACRVIVVTSGKGGVGKTTTTANLSVALAGRGHKVVAMDADIGL
                     RNLDLILGLENRIVYTLVDVVEGGCRLNQALV"
     gene            complement(423..1109)
                     /locus_tag="PL162_08530"
     CDS             complement(423..1109)
                     /locus_tag="PL162_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HQF92102.1"
                     /translation="MSDGEGRRDDMMLKGDARGLRLVVPEELAPGMMKESLKNVLGAA
                     RHILAGARVVIDLRGRACTEGEVLDILTHFVWPGGVTVISWISFHAETLRLLRASGFP
                     TGEPPMPGPTPPKGRPPLLLIPRSLRSGQRVEHGADVVVAGHVNDGAEIFAVGCVVVW
                     GRLQGLVHAGCAGDHGAQILVRSFEAPQYRIGSKVSYVDKDAPWWGKAVVLCLENDAP
                     VAHQIDVKNA"
     gene            complement(1106..2857)
                     /gene="mrdA"
                     /locus_tag="PL162_08535"
     CDS             complement(1106..2857)
                     /gene="mrdA"
                     /locus_tag="PL162_08535"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HQF92103.1"
                     /translation="MSRDRRTLDFRVRVLRTFVLGTLACLGLGLYSFQIIQGDKYIRL
                     AAENRLRLIRTTPPRGVIYDANGLSLAESVRMFEIRGYPLDLRREGVMEQVAALFRRH
                     GIPLDTQMLADAVDRQFWAPYRAVTLVTNLTLSQVADLVGDASFLPQLFPQPVWRRIY
                     PAGALAAHVTGYVGEITKEELQARPEQGYGGGDQIGKLGIEAYYEGILRGEMGEEAVE
                     VDARGRRQRQIAHRPPRQGKDIVLTFDLGAQRVASDLMAGKRGVLLAMDVHSGGIKVL
                     CPVPTYDVNPLTWGVTPSEWARLNQDPDRPMLNRAIGGNYPPGSPFKVVPALAGLVEG
                     VISPQTQVFCPGTFAVGNRIFKCWRRGGHGSENVHRALRDSCDTFFYQVGLWLGIDRL
                     SRWAGRFGVGALSGIDLPGEVPGNLGGEAWKRARLKDRWYQGDTANYSIGQGFVLMTP
                     LQVARMYAAVANGGYLVTPHLNRASDGLSVDMKLPQDALRLVQRGLESVVEGGTGSQA
                     RRFGVSVAGKTGTAQNPHGEDHAWFVGYAPVGHPRFVAVALVEGGGHGGAVAAPLVGE
                     LLSYLCREEETGGRSRP"
     gene            complement(2847..3314)
                     /locus_tag="PL162_08540"
     CDS             complement(2847..3314)
                     /locus_tag="PL162_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92104.1"
                     /translation="MTWVAGAFWYLQDLIQILGGGIFPVPELFFLAVFSAFVAGDESP
                     SQALWTVFVGGLLWDLRWTGLPGFTAALWAVVMGAVAIFWQAIPVQGRHGAIFLFIAA
                     GSHAALGGCRGLLYALSGFPIWPEILWQQIFGLPLVSIAALWLAHRKESEGVS"
     gene            complement(3311..4087)
                     /gene="mreC"
                     /locus_tag="PL162_08545"
     CDS             complement(3311..4087)
                     /gene="mreC"
                     /locus_tag="PL162_08545"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HQF92105.1"
                     /translation="MLWKELDKPWVQTLLAGGLGGVILALSLQFPDTRGAMALVGDAL
                     RWPEYPAVELRRGVAELRQWTAERRELQERLEALEGENLLLRSELGGRKDPLPPDGIG
                     AISARVTLRPPHRWWQEVRIDAGGRRGVRPGAPVLQNGFLVGRVSRVDQDEAWVDLIS
                     SAELLIPAVVEKTRDLGVMAGDGMGRTFLLYIPAERALEPRMAVSTALASEVLPPGIP
                     LGHIALSSDVREGFRYYQLSSGADLARLYDVSVFVGENKP"
     gene            complement(4303..5358)
                     /locus_tag="PL162_08550"
     CDS             complement(4303..5358)
                     /locus_tag="PL162_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HQF92106.1"
                     /translation="MFNWLSGLFCRDVGIDLGTANVVVYVRGKGIVNTEPSAIAVRRT
                     GRKGSGGVIAVGTSAKAMVGKTPTGVVTVRPLQHGVIADFDMTEAMVSHFLSRVNRGF
                     RLFARPRVVICVPACVTEVERRAVIDATLGAGAREAYVVEEPIAAALGAGLPIHEPRG
                     NMVVDIGGGTTEVAVLSLRGIVVHKALRDAGDEMDQAIVAMLRSNYTLLVGEITAEEI
                     KITLGSACPLGEELTMEVKGRDLMDGLPKAVEISSVEVREALNPSILRIEDMVKDALE
                     QTKPELARDIVDQGLVLTGGGALLRGMGKRLQKTLSIPVITAEQPLFSVALGVGKILE
                     DLDSRQGPMVALEKGAF"
     gene            complement(5527..6198)
                     /gene="radC"
                     /locus_tag="PL162_08555"
     CDS             complement(5527..6198)
                     /gene="radC"
                     /locus_tag="PL162_08555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HQF92107.1"
                     /translation="MTPLGLADLPPGERPRERLWEKGAGALSLAELLAILLGTGTRER
                     DVLALAGDLVEAFGGLSGLARAAGTELLAFRGLGKAKAAHVAAALELARRYQKELLPR
                     GERAGWQGRLEEIAAQVAHEEREFVYVLFLNRRGVLLEEGCLSFGGLEGAYLDMPYLF
                     RRGVRLDATRFVLAHNHPDGDPSPSQEDRALTEHVSRRLPLLGMELEGHFVLARGRCV
                     RVPPP"
     gene            complement(6195..>6401)
                     /locus_tag="PL162_08560"
     CDS             complement(6195..>6401)
                     /locus_tag="PL162_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQF92108.1"
                     /translation="GEEEILFSGDTLFARSVGRCDLPGGDEGALERSLGLLALLPDAL
                     RVFPGHGPGTTIGEERAQNPFWPR"
BASE COUNT         1236 a         2350 c         1785 g         1030 t
ORIGIN      
        1 gcaccaacgc ctgattcaag cgacaccccc cctccaccac atccaccaag gtgtaaacga
       61 tgcggttttc taagcccagg atgagatcaa ggttgcgaag gccgatgtct gcgtccatgg
      121 ccaccacctt gtgcccccgc cccgccaacg ccacagaaag gttggcggtg gtggtggtct
      181 ttcccacgcc gcccttccca gaggtcacca caatcactcg gcacgccatg cctacaccct
      241 ctccttatcg accccaaggc cctcgtcgct ccatcctcgt ccgacatgga gaaagcctct
      301 acggatgaag cgattaccgg cgtcggcgtc tccgccgcag gtctgtcatt ctgagatctt
      361 tttttagttg ctgaggaccg agggggcaaa ccccgcgaag tcatcatcgc ccacgccagg
      421 cgtcacgcgt tcttgacgtc tatctggtgc gccaccgggg cgtcgttttc caggcacaag
      481 accacggctt tcccccacca cggggcatct ttatccacgt agctcacctt gctcccaatg
      541 cgatattggg gcgcttcgaa ggatctcacc aggatctggg ccccatgatc tcccgcacac
      601 cccgcgtgca ccagcccctg gaggcgtccc cagaccacca cgcaccccac ggcgaagatc
      661 tccgccccgt cgttcacgtg ccccgccacc accacgtccg ccccgtgctc caccctctgc
      721 ccggagcgga gagaccgggg gatcaggagc aacggcgggc gccccttggg aggggtcggc
      781 cccggcatgg ggggctcccc cgtgggaaat cccgaggccc gcagtaggcg gagggtttcg
      841 gcatgaaaag aaatccatga gattaccgtc acacccccgg gccagacaaa gtgcgtgagg
      901 atatccaaaa cttccccctc ggtgcacgcc ctcccccgaa ggtcaattac cacccgggcc
      961 cccgccagga tatgccttgc cgcccccagg acgtttttta aactttcctt catcattccc
     1021 ggtgccaact cctccggcac gacgaggcgt aacccccgag cgtctccttt aagcatcatg
     1081 tcgtccctgc ggccctctcc atcgctcatg gtcggcttct cccccccgtc tcttcttctc
     1141 ggcacagata ggataggagc tcccccacca agggagccgc cacggctcct ccgtgccccc
     1201 ccccctccac cagcgccacc gccacgaagc gggggtgccc cacgggggcg taccccacga
     1261 accaggcgtg atcctccccg tgggggttct gggcggttcc cgtctttccc gccaccgaca
     1321 ctccgaagcg gcgggcctgg gaaccggttc ctccctccac cacgctttcc aacccccgct
     1381 gcacgaggcg cagcgcgtct tggggaagtt tcatgtccac cgaaaggcca tccgaggccc
     1441 ggttcaggtg gggcgtcacc aaataacccc cattggccac cgctgcgtac atgcgcgcca
     1501 cctgcagcgg agtcatgagg acaaagccct gcccgatgga gtaattcgcc gtatccccct
     1561 ggtaccagcg atccttaagg cgggcccgtt tccacgcctc ccccccgagg tttcccggca
     1621 cctcgccggg aaggtctatc cccgaaaggg cgccaacgcc aaagcgtccc gcccaccggc
     1681 tgagtcgatc gatgcccagc caaagcccca cctggtagaa aaacgtatcg caggaatccc
     1741 tgagggcccg atggacgttc tcgctgccgt gtcctccccg gcgccagcac ttaaaaattc
     1801 ggtttcccac ggcaaaggtg ccggggcaaa agacctgggt ctggggagag atcacccctt
     1861 ccaccagccc cgcaagggcg ggcactacct tgaagggaga ccccggcggg taatttcccc
     1921 cgatggctcg gttcagcatg ggacgatcgg gatcttgatt gagacgggcc cactccgagg
     1981 gggtcacccc ccaagtcagg gggttcacgt cgtaggtagg gacagggcac aggaccttga
     2041 tgccccccga gtggacgtcc atggccaaaa gcacgcccct cttccccgcc atgagatccg
     2101 acgccacccg ctgggccccc agatcgaacg tcagcacaat gtcttttccc tgccggggag
     2161 ggcgatgggc gatttgacgc tgacgacgcc cccgggcgtc cacctccacc gcctcctccc
     2221 ccatttcccc ccgcaaaatc ccctcgtagt aggcctcgat gcccaatttg ccgatctgat
     2281 cccctcctcc atacccctgc tccgggcggg cctgtagctc ttccttcgtg atctccccca
     2341 cgtagcccgt cacgtgcgcc gccagcgccc ccgccggata gatccggcgc cacacgggct
     2401 ggggaaagag ttggggtaaa aagctcgcgt cccccaccag gtcggccacc tgggacagcg
     2461 taaggttggt caccaaggtc accgcacggt agggggccca gaactgacga tccaccgcat
     2521 cggccaacat ctgggtgtcc aaggggatac catgccgccg aaatagggcc gccacctgct
     2581 ccatgacccc ttcccgccga aggtctaggg ggtaaccacg gatctcgaac atccggacgc
     2641 tttccgccag ggaaaggcca ttggcatcgt aaatcacccc tcgaggggga gtggtgcgaa
     2701 tcagcctgag gcggttttcc gccgccaagc gaatgtactt atccccctgg ataatctgga
     2761 aggagtagag ccccaggccc aggcacgcca gggtgcccag cacaaaggtc cgcagcaccc
     2821 tcacccgaaa gtcgagggtc ctgcggtcac gagacacctt cggactcctt tcggtgagcc
     2881 aaccaaaggg cggcgatgct caccaggggc agaccgaaaa tctgctgcca caggatttcc
     2941 ggccatatgg gaaagccgga gagggcgtaa agcagtcccc ggcacccccc cagggcggcg
     3001 tggcttccgg cggcgatgaa cagaaaaatg gccccgtgcc gcccctggac cgggatagcc
     3061 tgccagaaga tcgccaccgc ccccatgacc accgcccata gcgccgcagt aaaacccggc
     3121 aaccccgtcc atcgcaggtc ccacaggagc cctccgacaa aaaccgtcca cagggcctgg
     3181 gaaggagatt cgtccccggc gacgaaggcg gaaaagaccg ccaaaaaaaa cagttcgggg
     3241 acgggaaaga tccccccccc gaggatctgt atgagatcct ggagatacca aaaggccccc
     3301 gcaacccacg tcacggctta ttctctccca cgaagacgga aacatcgtag agccgagcca
     3361 ggtccgcccc agaggagagc tggtagtacc gaaagccctc tcggacatcg gaggaaaggg
     3421 cgatatgccc caggggaatg cccgggggta acacctcgct cgccagggcc gtggataccg
     3481 ccatacgggg ttccagggcc ctttctgcgg gaatatagag caaaaaggtg cgccccatgc
     3541 cgtctcccgc catcaccccc agatcccggg tcttttccac cacggcggga atcaggagct
     3601 cggcggaaga gatcaaatcc acccaggcct cgtcctgatc gacccgggac acgcgcccca
     3661 ccagaaagcc gttctgaagg acgggagctc cggggcgcac gccccggcgg ccccctgcgt
     3721 caatacgcac ctcctgccac caccgatggg gagggcgcag ggtcacccgg gccgatatcg
     3781 ccccgatccc gtcgggggga agggggtcct ttcgtccccc caattctgac cggaggagga
     3841 gattttcgcc ttccagcgcc tccagccgct cctggagctc ccggcgttcc gccgtccatt
     3901 gacggagctc cgccacgccc ctacgcagct ccactgcggg gtactcgggc caccgtaggg
     3961 cgtctcccac cagggccatc gccccccggg tatccggaaa ctggagggat aacgccaaaa
     4021 tgacccctcc cagtcctccg gcgaggagcg tctgcaccca cggtttgtcc aattccttcc
     4081 agagcatgcc aacctccccg cctggtgaaa tgaccatccc tcggcaaaaa tttccaccga
     4141 aacaagggcg catcgcggcc cgccattttt tctcgaagag agtccctccc gggctggcga
     4201 tggatgcccg gaatccctcc ccgtcctccg gcaagagatt caaaaagggc acaccccaca
     4261 cgacgggaag cgccctcctg gcctcactct ccccaggtcc tgctaaaacg cgcccttttc
     4321 cagggccacc atgggccctt ggcgggagtc caggtcttca aggatctttc ccactcccag
     4381 ggccacggaa aagaggggct gttcggcggt gatgacgggg atactcagcg ttttctgaag
     4441 cctctttccc atgccccgga gcaacgcccc tcctcccgtg agcacgagcc cctggtcaac
     4501 gatgtcccgg gccaactccg gtttggtctg ctccagggcg tctttcacca tgtcttcaat
     4561 gcgtaaaatg ctgggattta aggcttcgcg cacctccacg gagctgatct ccaccgcctt
     4621 ggggaggcca tccatgagat cgcgcccctt cacttccatc gtgagttcct cccccagggg
     4681 acacgcagag cctagcgtta tctttatctc ctccgccgtg atctccccca ccaacagggt
     4741 gtaattcgag cggagcatgg ccacaatggc ctggtccatc tcgtccccgg cgtcccggag
     4801 ggccttgtgg accacaatgc ccctcaggga gagcaccgcc acctccgtgg tgcctccgcc
     4861 gatatctacc accatgtttc cccggggctc gtggatgggg agccccgccc ccagggcagc
     4921 ggcgatgggc tcttccacca cgtaggcctc ccgggccccc gctcccaagg tggcgtcaat
     4981 gaccgcccga cgttccacct ccgtcacgca ggcggggacg cagatcacca cccgagggcg
     5041 ggcaaagagt cgaaaccctc tattaaccct agacagaaag tgggagacca tggcctccgt
     5101 catgtcgaag tcggcaatca ctccgtgctg gagggggcgc accgtcacca cccctgtggg
     5161 ggtcttgccc accatggcct tagccgaagt gcccactgcg atgacgcccc cgctcccctt
     5221 tcgtcccgtg cgacgcaccg cgatggctga aggttccgtg ttcacgatac ccttcccccg
     5281 aacgtagacc accacgttgg cggtgcctaa atcaatgccc acatcgcggc aaaacaagcc
     5341 gctaagccaa ttgaacaccg ggattccccc ccactttgct agctaaaatt ctttttcgcc
     5401 cctggacgcc cccgcaaaaa gggccctcgg acgcctcgtc ctaccgtgcc cccttatcgc
     5461 cgcaaggggt cgaaggggcc aggccccgca aagggccccg gcggtgtcga ctccgaaaga
     5521 gcccctctaa ggggggggca cccggacgca tcgaccccgg gccaggacga agtgcccctc
     5581 cagctccatg cccaaaaggg gcaatcggcg ggacacgtgc tcggtcaggg ctcggtcttc
     5641 ttggctgggg gatggatcgc catccgggtg attatgcgcc agcacgaagc gggtggcgtc
     5701 gagccgcacc ccccggcgaa agagataggg catgtccaga taggccccct ccaacccccc
     5761 gaaggaaagg catccctcct ccagaagaac ccctcggcgg ttcagaaaga gcacgtagac
     5821 gaactcccgc tcttcgtgcg ccacctgggc ggcgatctcc tccagacgcc cctgccaccc
     5881 cgcccgttcc ccccggggga ggagttcttt ttggtatctt cgggcaagct ccagggccgc
     5941 cgccacgtgg gccgccttgg ctttccccag ccctcgaaac gccaaaagct cggttcccgc
     6001 agcccgagcc agccccgaca gccctccaaa agcctccacc aagtcccccg ccagggccaa
     6061 gacgtcccgc tcgcgggtcc ccgtgcccag gagaatggcc aaaagctccg ccagggaaag
     6121 ggcccccgcc cccttttccc aaaggcgctc ccgggggcgc tcccccggag gaagatcggc
     6181 caggcccaag ggagtcaccg aggccagaag ggattctgag cgcgctcttc cccgatggtg
     6241 gtcccggggc cgtgccccgg aaaaaccctc agggcgtcgg gaagaagggc caaaagcccc
     6301 agggaacgtt ccagtgcccc ctcatccccc ccggggagat cacagcgccc cacgctccgg
     6361 gcaaacaggg tgtccccgga aaagaggatc tcttcctccc c
//
LOCUS       DAOOER010000083         6378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_12019_length_6378_cov_6.891349, whole genome shotgun sequence.
ACCESSION   DAOOER010000083 DAOOER010000000
VERSION     DAOOER010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 6378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6378
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12019_length_6378_cov_6.891349"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="PL162_08565"
     CDS             <1..462
                     /locus_tag="PL162_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HQF92109.1"
                     /translation="TQGILDVVRKIQTMISEEGLQGQVSVTVNQRGVTISMAEHALFE
                     RGSARVVPAGKRLLARMAPFLNAVSVPLAVEGHTDSLPVMGGVYETNWGLSSMRAATV
                     AAYLADVGGVDPLRLEAVGFSQYRPLVPNDSEPHRALNRRVDLVLLSHMSQ"
     gene            482..931
                     /locus_tag="PL162_08570"
     CDS             482..931
                     /locus_tag="PL162_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HQF92110.1"
                     /translation="MKRFMVFIVVGVVALLLGVGGGIYVGMSFFGQPEPVGGSAAVTA
                     MGPVVPVGDFTVNLSDKEPHIVKTTISLELLSEKGALVMADAGWQVRIRNEIVLVIKD
                     RRLDDLRSAEGVLDLAQDIKRRVNALLPLVEGQPPVVRVLFQDFISQ"
     gene            967..1983
                     /gene="fliM"
                     /locus_tag="PL162_08575"
     CDS             967..1983
                     /gene="fliM"
                     /locus_tag="PL162_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HQF92111.1"
                     /translation="MVPEVLSQSEIDALLSALSSGSVDIAAMTESAKEESIKRYDFRR
                     PDKFSKDQLRAIQMIHEAFARQMTTALSTMVRSMVSSEVVSVDQLAYDEFIRSLVQPT
                     TIAVLEMYPLTGNAVMEVNPHLVFSIIDRMLGGKGEPLRKARELTDIEKTVIERVLMR
                     LLELLEESWSTVVDVRFRFDGMESNPFFVQICPGTDMVLLVTMKVQVAETEGIINLCI
                     PYIVMEPMVDKLSSQHWFASTGRKSTEGSREALARKMQHVSVPLALELGHSVLSLQDV
                     LGLQVGDVIRLDGTAKDPIGVRVGNRVKFLASPGKIKGAFGAKIVKVLSEDEGAMLRE
                     REEE"
     gene            1986..>2467
                     /locus_tag="PL162_08580"
     CDS             1986..>2467
                     /locus_tag="PL162_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HQF92112.1"
                     /translation="MVDELLSQDEIDALLSGPGSSSAPPAAAGLSPDEGDVLGEVANI
                     IANAGGNVVGMLAGRAVTATVGETTVFPQGELAVRGGRQLLYTLSCDGFDNAPAAMAL
                     PERGALIMADLMMGGDGKDLPGEANDLYLSASQEGLSQMVGSAFTSISGLLKGQRLVP
                     A"
     assembly_gap    2468..2567
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2568..3271
                     /gene="fliN"
                     /locus_tag="PL162_08585"
     CDS             <2568..3271
                     /gene="fliN"
                     /locus_tag="PL162_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HQF92113.1"
                     /translation="SAFTSISGLLKGQRLVPANASGSLKEGEGWIPFGDRSPDDPVWG
                     VRVGVSVADVGDFDLWFLLPVDRARELVARIHAVLAPPAPTAPTKAAPAAPKPASAPS
                     PAPRATAAASPPSGGAPAPSRAAPPVDVRPAEFVPLQGEGGGALPANLDLIVDIPVRI
                     TVELGRTRKTIGEVLNMAPGSVVELEKMAGEPVDVLVNGKLVARGEVVVIDESFGVRV
                     TEIVSKTERIRSMGM"
     gene            3302..3661
                     /locus_tag="PL162_08590"
     CDS             3302..3661
                     /locus_tag="PL162_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQF92114.1"
                     /translation="MPRVLIVDDAAFMRMMLKDILTKNGYEVAGEAENGKVAVSMYQE
                     LKPDVTTMDITMPEMDGISAVKEIKKANPGAKIVMVSAMGQQAMVIEAIQAGAADFIV
                     KPFQPDRVLEALGKAIS"
     gene            3825..4094
                     /locus_tag="PL162_08595"
     CDS             3825..4094
                     /locus_tag="PL162_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92115.1"
                     /translation="METYLVRVAIALALLALGGWGLASWAPFRRQGKGLELLAVLPLG
                     RDLVRVVGCGPEVVALLSSRGGTTVIGRWSREEWQVSCGRRDMDA"
     gene            4067..4849
                     /gene="fliP"
                     /locus_tag="PL162_08600"
     CDS             4067..4849
                     /gene="fliP"
                     /locus_tag="PL162_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870061.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HQF92116.1"
                     /translation="MRAPGYGRLGLALILGILMAGGGATGAWGAPEAPTLPIPALEFG
                     VKAAGSPQDVVLSLQMLALLTVLSMAPAIVLMLTSFTRILVVLSLVRNALGLQQVPPN
                     QVLVTLALFLSFFTMAPVWDRVYDGALSPYMKGDLSAQEAWDRTIAPLKDFMMRQTRQ
                     EELSLMISMAKEERPRNAEDVSMRTLMPAFVLSEIKTAFQMGIVIFVPFIVVDMVVAS
                     VLMAMGMIMLPPMMISLPFKILLFVLADGWNLVISSLVTSIR"
     gene            4859..5128
                     /gene="fliQ"
                     /locus_tag="PL162_08605"
     CDS             4859..5128
                     /gene="fliQ"
                     /locus_tag="PL162_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HQF92117.1"
                     /translation="METLSMFDILRQAVWVVIMASMPALVVAMLVGVLLGVVQTATSI
                     QEQTLAFVPKIIAIFAALVLFGPFMFVQVGDFARFVLKNLHRFVQ"
     gene            5155..5907
                     /locus_tag="PL162_08610"
     CDS             5155..5907
                     /locus_tag="PL162_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HQF92118.1"
                     /translation="MVLYLLLSLRFLGVLTAAPPLAGVALPVSARFLLAALLAFFALP
                     GTAPLPDMGGVGSLPGMVLASGREFLLGSLFGFLVGAPLYMLQMTGRVVGMQMSFEMA
                     SVLDPFTQNQVSVVEQLHFVTGMWFFFLWDGHLLVLQGVAESLRLIPVGSLGLFPRSD
                     MGLGLWLSQILGAAIRMALPFFGALLLADVGLGFVARTVPQMNIFVLGLPLKAGLGFF
                     LLTVVLPMAVDTVHGMLDWTWRSALEALIGWR"
     gene            5912..>6378
                     /locus_tag="PL162_08615"
     CDS             5912..>6378
                     /locus_tag="PL162_08615"
                     /inference="COORDINATES: protein motif:HMM:NF013479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HQF92119.1"
                     /translation="MIRRRFDLQLLGEDRSEPATPRKRQKAREEGQVARSQDLGAAVV
                     LVAGLLCIHMFFLWGFRELSGFLRGMISLVGSPRLHGELWPGEVILLGMKAFLLLWLP
                     LAFGAFLMGLAAQVAQVGWKLSPKALHLKFDRLNPISGLKKIFALRSLVELLKG"
BASE COUNT         1005 a         1825 c         2179 g         1269 t
ORIGIN      
        1 acccagggca tcctcgacgt ggtgcgcaag atccagacca tgatcagcga agaggggctc
       61 caggggcagg tgtcggtgac ggtgaaccag aggggagtca ccatctccat ggcggagcac
      121 gccctcttcg aaaggggatc ggcccgggtg gtgcccgcag gaaagcgcct tttggcccgg
      181 atggccccct ttctcaacgc ggtgtcggtg cccctggcgg tggaggggca tacggacagc
      241 cttcccgtga tgggaggggt gtacgagacg aattgggggc tctcctccat gcgggcggcc
      301 accgtggcgg cctacctggc ggatgtgggg ggggtggatc ctctgcggct cgaggcggtg
      361 ggctttagcc aatatcgccc cctggtgccc aacgactcgg agccccacag ggccctaaac
      421 cgccgggtgg atttggtcct tttgtcccat atgtcccagt aggtcgagag ggggaccagc
      481 ggtgaaaagg ttcatggtct ttatcgtggt gggcgtggtg gcccttctgc tgggggtggg
      541 cgggggcatc tacgtgggga tgtcattttt cggccagccg gagcccgtgg ggggatccgc
      601 cgccgtgacg gcgatgggac ccgtggtgcc cgtgggagat ttcaccgtca acctttcgga
      661 caaggagccc cacatcgtca agaccaccat ctccctggag ctcctttccg aaaagggagc
      721 tctggtgatg gccgacgcgg ggtggcaggt gcgcataaga aatgagatcg tgctggtgat
      781 caaggatcgt cgcctggacg acctgcggag cgcggaaggg gtcttggatc tggcccagga
      841 catcaagcgc cgggtgaacg ccctccttcc cctggtggag ggccagccgc cggtggtgcg
      901 ggtcttgttc caagatttca tcagccagtg acggaagtcc ttccccgata gagaggtggt
      961 gtgcccatgg ttccggaggt cctctcccaa agcgagatag acgccctatt atctgctctg
     1021 agcagcggca gcgtggacat cgctgccatg acggagagcg ccaaggaaga atcgataaag
     1081 cgctacgact tccggcgccc ggacaagttc agcaaggacc agctccgggc cattcagatg
     1141 atccacgagg ccttcgcccg gcagatgacc acggcccttt ccaccatggt gcgttccatg
     1201 gtatcctccg aggtggtgtc ggtggaccag ctggcctacg acgagttcat ccgatccctg
     1261 gtgcagccca ccaccatcgc ggtgctggag atgtacccct tgacgggaaa cgcggtgatg
     1321 gaagtgaacc cccacctggt gttttccatc atcgaccgca tgctcggcgg caagggggag
     1381 cccctgagga aggcccggga gctcacggat atcgaaaaaa cggtcatcga gcgggtgctc
     1441 atgaggctcc tggagctgct ggaagagagc tggagcacgg tggtggacgt gcgctttcgc
     1501 ttcgacggca tggagagcaa tccgtttttc gtgcagatct gtccggggac ggacatggtg
     1561 ctcctggtga ccatgaaggt gcaggtggcg gagaccgagg ggatcatcaa cctctgcatc
     1621 ccctacatcg tcatggagcc catggtggac aagctgagct cccagcactg gtttgcctcc
     1681 acggggcgta agtccaccga ggggtcgcga gaggccctgg cccgaaagat gcagcacgtc
     1741 tccgtgcccc tggcgctgga gctggggcac tcggtgctga gcctccagga cgtgttaggg
     1801 ctccaggtgg gggacgtgat acgcctggac ggcaccgcca aagatcccat cggggtgcgc
     1861 gtgggaaacc gggtgaagtt cctggcctct ccggggaaga tcaagggagc cttcggggcc
     1921 aagatcgtca aggtcctctc ggaggacgaa ggggcgatgc tccgggagcg tgaggaggaa
     1981 tagccatggt ggacgaactc ctgagccagg atgaaattga tgcgttactg agcggtccgg
     2041 gctcctccag cgctcctccg gcggcggcgg ggctctcgcc ggacgagggc gatgtcttgg
     2101 gcgaggtggc gaacatcatc gccaacgccg ggggcaacgt cgtcggcatg ctcgccggtc
     2161 gagcggtcac ggccacggtg ggagaaacca cggtctttcc ccagggggag ctcgcggtcc
     2221 ggggggggcg gcagctcctc tacaccctgt cctgtgacgg cttcgacaac gccccggcgg
     2281 ccatggcact tcccgagcgg ggggcgctga tcatggcgga cctcatgatg gggggcgacg
     2341 gtaaggatct tcccggggag gcgaatgacc tctacctgag cgcctcccag gaggggctca
     2401 gccagatggt ggggtcggct tttaccagca tcagcgggct cctcaagggg caacgcctgg
     2461 tgcccgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggt cggcttttac
     2581 cagcatcagc gggctcctca aggggcaacg cctggtgccc gccaacgctt cgggatccct
     2641 gaaagagggg gaagggtgga ttccctttgg agaccgctcc cccgacgatc ccgtctgggg
     2701 ggtgcgggtg ggggtctccg tggcggatgt gggggacttc gacctctggt tcctcctccc
     2761 ggtggatcgg gcccgggagt tggtggcccg catccacgcg gtactggccc ctcccgcccc
     2821 taccgccccg acaaaggccg ctccggcggc gcctaaaccg gcttctgctc cgtcccccgc
     2881 tccccgggct acggcggcgg cgtcgcctcc ttccggaggg gcccccgccc cttcccgggc
     2941 ggcccctccg gtggatgtgc gtccggcgga gttcgtcccc ctccagggag aagggggcgg
     3001 agcccttccg gccaatctag atctgatcgt cgacatcccg gtgaggatca ccgtcgaact
     3061 ggggcgtacc cgaaagacca tcggcgaagt gttgaacatg gcgccgggtt ccgtggtaga
     3121 attggagaag atggcgggag agccggtgga tgttttggtc aatggcaaac tggtggcccg
     3181 gggcgaagtg gtggtcatcg acgagagctt cggcgtgagg gtcacggaga tcgtgagcaa
     3241 gaccgagcgc attcgcagca tgggcatgtg atgcggggga tatcacaggg agggacgaca
     3301 gatgccgaga gtgttgatcg tggatgatgc agccttcatg cgcatgatgt taaaagacat
     3361 tctcaccaag aacggctacg aggtggccgg agaggcggag aacgggaagg ttgccgtttc
     3421 catgtatcag gagctcaaac ctgatgtgac caccatggac atcaccatgc ccgagatgga
     3481 tgggatttcc gcggtgaagg agatcaaaaa ggccaacccc ggagcgaaga tcgtcatggt
     3541 gagcgccatg gggcagcagg ccatggtcat cgaggccatc caggccgggg cggcggattt
     3601 tatcgtcaag cccttccagc cggatagggt gctggaggct ctggggaagg ccatctccta
     3661 gggggcaaag tcgaggttcc gcccccggcg ggcctgggga ggtagtgagg cggggcctga
     3721 ggcgtggtgg ggccccgctt gttcgttgtg gcgcctctct tgcgcttttg gtcgaggccg
     3781 ctttcttcgg gtccgatggc ggtccggggt gaggagggga agggatggag acatatctgg
     3841 tgcgggtggc catcgccctg gccctcctgg ccctgggagg gtggggcctg gcgtcctggg
     3901 ctcctttccg gaggcaaggg aagggtttgg agctgttggc ggtccttccc ctgggacggg
     3961 acctcgtgcg ggtggtgggg tgcggtcccg aggtggtggc gttgctctcc tctcgagggg
     4021 gaacgacggt aatcgggcgc tggtcccggg aagagtggca ggtctcatgc gggcgccggg
     4081 atatggacgc ctagggctag ccctgatcct ggggatcctg atggcggggg gaggagcgac
     4141 gggagcctgg ggagcccccg aggcccccac cttgcccatc ccggcgttgg agttcggggt
     4201 gaaagccgcc gggtcgcccc aggacgtggt cctctccctc cagatgctag ccctcctcac
     4261 ggtgctcagc atggctcctg ccatcgtcct catgctgacg agtttcaccc gaatcctggt
     4321 ggtgctcagc ctggtgcgca acgccctggg attgcagcag gtccctccaa accaggtact
     4381 ggtgactctg gccctcttcc tgtcgttttt caccatggcc cccgtgtggg accgggtcta
     4441 cgatggggcc ctttccccct acatgaaagg agatctctcc gcccaagagg cgtgggatcg
     4501 aaccatcgcc ccgctaaagg attttatgat gcgccaaact cgccaggaag agctttccct
     4561 catgatctcc atggctaaag aagagcgccc ccggaacgcg gaagacgtct ccatgaggac
     4621 cctcatgccc gctttcgtcc tgagcgagat caaaacggcc tttcagatgg ggatcgtcat
     4681 cttcgtgccc tttatcgtgg tggacatggt cgtggccagc gtcctcatgg ccatgggaat
     4741 gatcatgctc ccccccatga tgatctccct tcccttcaag atattgcttt ttgtgctggc
     4801 cgacggctgg aacttggtga tttccagcct ggtgaccagt atccggtagg ggggcgccat
     4861 ggagactttg agcatgtttg acatcctgag gcaggcggta tgggtggtca tcatggcgtc
     4921 catgcccgcc ctcgtggtgg ccatgctggt gggggtcctc ctgggggtgg tgcagacggc
     4981 cacctccatt caggagcaga cgttggcctt tgtgcccaag atcatagcca tcttcgcggc
     5041 cctcgtgctc tttggccctt tcatgttcgt gcaggtgggt gatttcgccc ggtttgtcct
     5101 gaagaacctg caccgcttcg tccaatgatc gcctccgccc ctccggagtc tttgatggtg
     5161 ctttaccttc tgctgagcct gcgattcctg ggggtgctca cggcggcgcc tcccctggcg
     5221 ggggtggctc tgcccgtttc ggctcgcttc cttctggcgg ccctcttggc cttttttgcc
     5281 ctccccggga cggctcccct ccccgatatg gggggggtgg gatctcttcc cggcatggtc
     5341 ttggcctccg gccgggagtt cctcctgggg tctttgttcg gttttctcgt gggggcgccc
     5401 ctctacatgc tccagatgac cggccgggtg gtggggatgc agatgagctt cgagatggcc
     5461 agtgtcctgg atccctttac ccaaaaccag gtgtccgtgg tggagcagtt gcacttcgtc
     5521 acggggatgt ggtttttttt cctctgggac ggccatctcc tggtcctcca gggcgtggcg
     5581 gaaagcctcc ggctgatccc cgtgggaagc ctggggcttt ttccccggag cgacatgggt
     5641 ttggggcttt ggctcagcca aatcctcggg gccgccatcc ggatggccct gccctttttc
     5701 ggagcccttc tcctggcgga tgtggggctg ggctttgtgg cccgcacggt gccccagatg
     5761 aatatcttcg tgctggggct tcccctgaag gccggattgg gattttttct cctcacggtg
     5821 gtgcttccca tggccgtgga cacggtccac ggcatgttgg attggacatg gcgtagcgcc
     5881 ctggaagccc tgatcgggtg gcgatgatgg ggtgatccgg cggcgtttcg acctgcagct
     5941 cctgggagaa gatcggtcgg aacccgccac tccccgaaag cgtcagaaag cccgggaaga
     6001 ggggcaggtg gcccggagcc aggacctggg agcggcggtg gtgctcgtgg cggggttgct
     6061 ctgcatccac atgttcttcc tctgggggtt tcgggagctt tcgggattcc tgaggggcat
     6121 gatcagtctg gtgggttcgc cgcgcctcca tggagagctc tggcccggag aagtgatcct
     6181 tctggggatg aaggcttttt tgctcctgtg gcttcccttg gcctttgggg cctttttgat
     6241 ggggttagcg gcccaggtgg cccaggtggg atggaagctc tcgccgaagg cgctacacct
     6301 gaaattcgac cgattgaacc ccatttccgg cctcaaaaag atcttcgccc ttcgctccct
     6361 ggtggagctc ctcaaagg
//
LOCUS       DAOOER010000084         6328 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_12127_length_6328_cov_8.132473, whole genome shotgun sequence.
ACCESSION   DAOOER010000084 DAOOER010000000
VERSION     DAOOER010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12127_length_6328_cov_8.132473"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="PL162_08620"
     CDS             complement(<1..632)
                     /locus_tag="PL162_08620"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HQF92120.1"
                     /translation="MKSPVSFRVVAEDPVTGARAGELHTPHGVIETPVFMPVGTQGTV
                     KAMAPFELVEMGASMVLGNTYHLWLRPGSELVASLGGLHRFMGWSRGLLTDSGGFQVF
                     SLGELRTVSEEGVAFRSHIDGKRCFLSPEDSMRIQEELGSDIAMCFDECVALPASPER
                     SRDALERTLRWAERCQRSHGREDQALFGIVQGATFPDQRRECAERMTRMD"
     gene            complement(629..1423)
                     /locus_tag="PL162_08625"
     CDS             complement(629..1423)
                     /locus_tag="PL162_08625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HQF92121.1"
                     /translation="MGTLVDTHCHLDMDSFDPDREAVLDRAASCGVGRMVTVGTDGAS
                     SLKALDLALRHTSRGVFATVGVHPHEASSVAEGLPGALRDLARHDRVVAVGETGLDFF
                     YDHSPREVQKKVLAFHVSWALEVRKPLVVHVRDAFDEALDILKSEGAAGSGGVFHCFS
                     GTWEQAVAALDLGFYLSFAGPVTFSKGEALRDVASRIPRDRLLCETDAPYLAPRPFRG
                     KRNEPAHVARIYDRLAEVRREARVELEAAIYRNAEALFRWAGEKAP"
     gene            complement(1426..3351)
                     /gene="metG"
                     /locus_tag="PL162_08630"
     CDS             complement(1426..3351)
                     /gene="metG"
                     /locus_tag="PL162_08630"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HQF92122.1"
                     /translation="MTDKGFYITTPIYYVNDVPHIGHAYTTVAADVLSRHHRLRGRDT
                     YFLTGTDEHGQKIQRAAMERGLTAQELADRTVVNFQDLWKVLGIEYDDFIRTTEERHE
                     RVVQHIFSKLLAQGDIYKGSYEGWYCVPCETYVPESQMGEGRTCPDCKRKLEKMTEES
                     YFFRLSKYADPLLEFYEAHPEAVQPRGRYNEIVSFIRGGLMDQSISRTTISWGVPVPE
                     NPRHVVYVWFDALINYLSALGYPDEGGLWERYWPAVHHLVGKDIIRFHSVIWPAMVMA
                     LGLNPPVMVFAHGWWTVEGEKMSKSKGNVVDPFDMARRYGADAFRYFLLREVPFGLDG
                     DFSEQALVQRINSDLANDLGNLLHRTLQMVNQFCDGEVPASKALHPEDEALCREAEEA
                     VEAVDRALESFAFDEALKVLWGLVARGNKYIDETMPWRLGKEGDRDRLGTVLGTLAQT
                     LRIVALLVTPFMPSTGQRMWQQLGLRGAPDKITSPAWGGYPAGTLVHKGEVLFPRIDL
                     DLWQKERQARAEEDKNPKDPGDHEAEVTIDDFRKIELRVALVTAVEPVPKADKLYKMS
                     LDLGYEKREIVSGIREFYPPEDLVGKRIVVICNLKPAKLRGVMSCGMLLAAERFDGKN
                     LALLTVDRDMELGARIH"
     gene            complement(3497..4357)
                     /gene="rsmI"
                     /locus_tag="PL162_08635"
     CDS             complement(3497..4357)
                     /gene="rsmI"
                     /locus_tag="PL162_08635"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HQF92123.1"
                     /translation="MPLIVVPTPIGNMEDMTLRGLRVLREAQVIACEDTRRTLQLLNF
                     HGIRTPMVSCHEHNERSRARELLGRVRRGETVALVSDAGTPGLSDPGAFLLSAALAAG
                     LPVDVLPGATALVPGLLLSGLPPQPFTFYGFLPEGGSDRRRALEALKTHPWTLVFYLS
                     PHKAERHLEDLASLWGDRAAVLVREISKIHQEAVAGTLPELLERARGGFRGELVLVVS
                     GAPDSPPDDEMWRREARTMAQGGHPWRDIAKELGERYGIPKNRVKALLFAEDEGAEEE
                     EIPGQGLANG"
     gene            complement(4348..5196)
                     /locus_tag="PL162_08640"
     CDS             complement(4348..5196)
                     /locus_tag="PL162_08640"
                     /inference="COORDINATES: protein motif:HMM:NF013856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQF92124.1"
                     /translation="MMEEHLRREEDESLCHEDPAAEGGRFAFSDAPSPEEDLPPSRDD
                     YLQGALRLWQPSRGPRANVDTVLLGAFARARKGDRVLDLGCASGAVALHLAWRFPGVT
                     VLGVDIQRDLVALAERNAQENAQEDRVSFVAGDLRNPQEFCAPESFDVVVANPPYGDG
                     SLSRPSPSVPAAMARHDLCCSLSQVARAAAFALRFGGRFYAVFRAARISALLSTWQHF
                     RLEPKRILPVYPKAGKDASVVLVGAVKGARPGGRMESPMILQGEDGRFTPSLLQAYAR
                     EGLPCR"
     gene            complement(5193..>6328)
                     /locus_tag="PL162_08645"
     CDS             complement(5193..>6328)
                     /locus_tag="PL162_08645"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HQF92125.1"
                     /translation="PEERAWESRVVALRGEVALGEERLRSAEALVRRETAALAQADEA
                     MTAFTEDLRRIDEEEASRKKSLRDVGQKIWDLWRENKLLEERQSRLLDRRTALDRRTA
                     RARLRARAAAERMEGARAAAERMERMGRDLTRELDDLSALWEGQFPYPGQEGFEEPRT
                     LEELRRTLRERERALRELGEVDGGVLSEDRSLEERVLFLQDQTQDVTRAREELQAFIS
                     DTDAQAGALFSQALQGIDRRFNELFRRLFGGGEGRLMLTEGLSLWDAGVEVMARPPGK
                     TPQHLGQLSGGEQSLTGIALLFAAMEVAGAPLAVLDEVDAALDEANLKRFAALAEECA
                     RTRQLIVMTHRRYTMERAQVLYGVTMSEPGLSQVVGVRLEDWT"
BASE COUNT         1127 a         2185 c         1913 g         1103 t
ORIGIN      
        1 aagtccatgc gggtcatgcg ctcggcgcac tcccgtcgct gatccggaaa agtcgccccc
       61 tggacgatgc cgaagagggc ctggtcttcc ctcccatggg acctctggca gcgctcggcc
      121 caccgcaggg tacgttccag ggcgtcccgg gagcgttccg gagacgcggg gagggccacg
      181 cactcgtcga agcacatggc aatgtcgctc cccagctcct cctggatgcg catggagtcc
      241 tctggagaca aaaaacatcg cttcccgtcg atgtgggatc ggaaggccac gccctcttcg
      301 gagacggtgc gaagctctcc cagagagaac acctggaatc ctccgctatc cgtgaggagt
      361 ccccgggacc accccataaa gcggtggagc ccccccaggg acgccaccag ctccgagccc
      421 gggcgaagcc ataggtgata agtgttgccc aaaaccatgg aagctcccat ctccaccagc
      481 tcaaaggggg ccatggcctt caccgtcccc tgggtcccca cgggcatgaa cacgggggtt
      541 tcgatgactc cgtggggcgt gtggagctcc cccgcccggg ccccggtgac ggggtcttct
      601 gccaccaccc ggaaggacac ggggctcttc atggggcctt ctccccagcc caccggaata
      661 aggcctcggc gttgcggtag atggcggctt ccagctcgac ccgggcctcc cgccggacct
      721 ccgccagccg gtcgtagatt cgggccacat gggcgggttc gttcctcttg cctcgaaagg
      781 ggcgaggagc cagatagggg gcgtcggtct cgcagaggag acgatcccgg gggatacggg
      841 aagccacgtc tcgtagggcc tcacccttgg aaaacgtgac gggtcctgcg aaggataggt
      901 aaaatcccag gtccaaagcc gccaccgcct gctcccaggt gccggaaaag cagtgaaaaa
      961 ccccaccgct tccggcggcc ccttccgatt tcagaatgtc cagggcttcg tcgaaggcat
     1021 cccgcacgtg caccacgagg ggctttctca cctccagggc ccaggacacg tggaaggcta
     1081 agaccttttt ttgcacctcc cggggggagt ggtcgtagaa gaagtcgagc cccgtctccc
     1141 ccacggccac cacccggtcg tgccgggcga gatcgcgaag agccccgggt agcccctccg
     1201 ccacggagga ggcctcgtgg ggatggaccc ccactgtggc aaagaccccc cgggaggtgt
     1261 ggcgaagggc cagatccaag gctttcaggc tcgacgcccc gtccgtgccc accgtcacca
     1321 tccgccccac tccgcatgag gcggcgcgat ccagcaccgc ctcccggtcg gggtcgaagg
     1381 agtccatatc caagtggcag tgggtgtcca ccaaagttcc catcgtcaat ggatccgcgc
     1441 cccgagctcc atatctcggt ccacggtgag aagggccaag tttttcccgt cgaaccgctc
     1501 cgccgccagg agcatgccgc agctcatcac tccccggagc ttggcgggct tcaggttaca
     1561 gataaccacg atgcgctttc ccaccaagtc ctcggggggg tagaactccc ggatgccgga
     1621 gacgatctcc cgcttctcgt accccagatc cagcgacatc ttgtagagct tatccgcctt
     1681 gggcacgggc tccaccgccg tcaccagggc cacccggagc tcgatcttcc ggaagtcgtc
     1741 gatggtcact tccgcctcgt gatccccggg gtctttgggg tttttatcct cttcggctct
     1801 cgcctggcgc tccttctgcc acagatccag gtcgatgcgg ggaaagagca cttctccttt
     1861 gtggacgagg gtgcccgcag ggtaccctcc ccaggcgggg gaagttattt tgtccggggc
     1921 tccccttagg ccgagctgct gccacatgcg ctgccccgtg gagggcatga agggagtcac
     1981 gaggagcgcc acgatcctca aagtctgcgc caacgtcccc aacacggtgc ccagccggtc
     2041 ccgatcgccc tcttttccca ggcgccaggg catggtttcg tcgatatact tgttcccccg
     2101 ggccacgagg ccccaaagga ccttgagggc ttcgtcgaag gcaaaggatt ccagggcccg
     2161 atccaccgcc tccaccgctt cttccgcctc acgacagagg gcttcgtctt ccgggtgcag
     2221 ggccttcgag gcggggacct cgccatcgca gaactggttc accatctgca gcgtgcgatg
     2281 cagcaagttg cccaggtcgt tggccaggtc gctgttaatg cgctgaacca gagcctgttc
     2341 cgagaagtcc ccgtccaggc caaagggcac ctcccggagc aagaagtatc ggaaggcgtc
     2401 cgccccgtaa cggcgggcca tgtcgaaggg gtccaccacg ttacccttgg acttggacat
     2461 cttctccccc tccacggtcc accacccgtg ggcaaagacc attaccggag gattcagtcc
     2521 cagggccatc accatggcgg gccagatgac ggagtggaaa cggatgatgt ctttgcccac
     2581 caagtggtgc accgcaggcc agtagcgctc ccaaagccct ccctcgtcgg ggtaacccag
     2641 ggcggagaga tagttgatga gggcgtcgaa ccagacgtag accacgtgcc gggggttctc
     2701 cggcacgggc acgccccacg aaatggtggt ccgggagatg gactgatcca tcaaccctcc
     2761 ccggatgaag ctgacaatct cgttgtatcg cccccggggc tgcaccgcct cgggatgagc
     2821 ctcatagaac tcaagcagcg ggtcggcgta tttggatagg cggaagaagt agctctcctc
     2881 cgtcatcttc tccagcttcc gtttgcagtc cggacaggta cgtccttccc ccatctggga
     2941 ttcggggacg taggtctcgc agggaacgca ataccacccc tcgtagcttc ccttgtagat
     3001 gtccccctgg gccaggagct tggagaagat gtgctggacc acccgttcgt gccgctcctc
     3061 cgtggtccgg atgaagtcgt cgtactcgat gcccagcacc ttccataggt cctgaaagtt
     3121 gaccaccgta cggtcggcaa gctcttgggc ggtcaacccc ctctccatcg cggcgcgctg
     3181 gattttctga ccgtgctcgt cggttccagt aaggaaatag gtgtcgcgcc cccggagccg
     3241 gtggtgccgg gagagcacgt cggcggccac ggtggtgtag gcgtggccga tgtggggcac
     3301 atcgttaacg tagtagatag gcgtggtaat atagaaaccc ttgtcagtca tggtatccct
     3361 cctaaaaaag atttccgcac cgcccggact gataaacccg ggaaggactt ctcgcattgc
     3421 tccaagggcc ctcgtccccg ccgatggggg gccatggcgt cttttcctat ggccggggaa
     3481 aggggcgcca tccgccctac ccgttagcta acccctggcc cgggatctcc tcctcttccg
     3541 ccccttcgtc ttcggcaaat aaaagagcct tgacgcgatt tttcgggata ccataccgct
     3601 cccccagctc tttggcaata tcccgccagg gatgtccccc ttgggccatg gtccgagcct
     3661 ctcgtcgcca catctcgtca tccggagggc tatccggggc ccccgatacc accaggacca
     3721 gctcccctct gaaccctccc cgcgcccttt ccaggagttc agggagggtt cccgccaccg
     3781 cttcctggtg gatcttgctg atctcccgca ccaggaccgc cgcccgatct ccccagaggg
     3841 aggccaggtc ctccagatgg cgctccgctt tgtggggcga caggtaaaag accagggtcc
     3901 acggatgggt tttcagggcc tccagggcgc gccgccgatc gcttcccccc tcggggagga
     3961 agccgtagaa cgtgaacggc tggggaggaa gacccgaaag aagtagccca ggcacgaggg
     4021 ccgtagcccc cggaaggacg tccacgggaa ggcccgccgc cagggccgcc gaaaggagaa
     4081 aggccccggg atccgaaagc cccggggtcc ccgcatcgga gaccaaagcc accgtctcgc
     4141 cccttcgcac tcgacccagc aactcccggg cgcgggaacg ctcgttatgt tcatgacacg
     4201 agaccatggg cgtccggata ccgtgaaagt tcagcagctg gagagttcgt cgggtatcct
     4261 cacaggcgat gacctgggcc tctcgcagga cccgcagccc tcgaagagtc atgtcttcca
     4321 tgtttcctat gggagtgggt accacgatca gcggcacggc aagccctccc gggcgtaggc
     4381 ttggagaagg gagggggtga agcggccgtc ttctccctgg aggatcatcg gagattccat
     4441 acgacccccg gggcgggccc ccttcaccgc ccccaccagg accaccgacg catcttttcc
     4501 cgccttggga tagacgggaa ggatcctctt gggctccagg cgaaagtgct gccacgtcga
     4561 taggagggcc gaaattcgcg ccgcccggaa caccgcgtaa aagcgtcccc cgaagcgcag
     4621 ggcaaaggcc gccgcccggg ccacctggga tagggaacaa cataggtcgt ggcgcgccat
     4681 cgccgccggc acggaggggc tggggcggga aaggcttccg tcgccgtagg gcggattggc
     4741 caccaccacg tcgaaggact cgggggcaca gaactcctgc gggttccgca ggtcccccgc
     4801 cacgaaagac acccggtctt cctgggcgtt ctcctgcgcg ttccgctccg ccagcgccac
     4861 caggtcccgc tggatatcca cccccaggac cgtcactccg ggaaagcgcc acgccagatg
     4921 cagggccacg gcccccgaag cacaccccag atcgagcacc cggtccccct ttcgggcccg
     4981 ggcaaaggcc cccaaaagca ccgtgtccac gttggcccgg ggtccccggg agggttgcca
     5041 aaggcgcaac gccccctgga ggtagtcgtc ccgcgagggc gggaggtcct cctccgggga
     5101 gggcgcatcg gaaaaggcga agcgccctcc ctcggcagcg gggtcctcgt ggcaaaggct
     5161 ttcgtcttct tctcgccgaa ggtgttcttc catcatgtcc aatcctccag ccggactccc
     5221 accacctggg aaaggccagg ctcgctcatg gtgacgccgt aaagcacctg ggctcgctcc
     5281 atggtgtagc gccgatgcgt catgacgatc aactgacggg tccgggcaca ttcctccgcc
     5341 agggcggcga agcgtttgag gttcgcctcg tccaaggccg catccacctc gtccagcacc
     5401 gccagagggg ctccggccac ctccatggcg gcaaagagga gggctatgcc cgtgagggat
     5461 tgttcccctc cggagagttg ccccaggtgc tggggcgttt tccccggagg tcgggccatc
     5521 acctccaccc ccgcatccca gagagaaagg ccctccgtca gcatgagccg tccctctccc
     5581 ccgccaaaaa gcctccggaa gagctcgttg aagcgccgat ctatgccctg gagcgcctgg
     5641 gaaaaaagcg cccccgcctg ggcgtcggtg tccgatatga aggcctggag ttcttcgcgg
     5701 gccctggtga cgtcctgggt ttgatcctgg aggaatagca ccctctcctc caaagatcgg
     5761 tcttcggaga ggactccccc atccacttct cccagctccc ggagggccct ttccctttcc
     5821 ctcaaggtcc gccgaagttc ttccagcgta cggggctctt cgaagccctc ctggccgggg
     5881 tagggaaatt gcccctccca gagggccgaa aggtcgtcga gctcccgggt tagatcccgc
     5941 cccatacgtt ccatccgctc cgctgcggcc cgagcccctt ccatccgttc cgccgcagcc
     6001 cgcgctcgaa ggcgagcccg ggccgtgcgc cgatccaggg ccgtgcgccg gtccaggagg
     6061 cgggattggc gctcttccag aagcttattc tcccgccaga gatcccagat cttttgtccc
     6121 acgtcccgga gggatttctt ccgagaggct tcttcttcgt cgatcctacg gagatcttcc
     6181 gtgaaagccg tcatagcttc gtcggcctgg gccagagccg ccgtctctcg acgcaccagc
     6241 gcctcggcgc tgcggagcct ttcttccccc agggccacct ctcctcgcag ggccaccacc
     6301 cggctctccc aagcccgctc ttcgggaa
//
LOCUS       DAOOER010000085         6289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_12212_length_6289_cov_7.178697, whole genome shotgun sequence.
ACCESSION   DAOOER010000085 DAOOER010000000
VERSION     DAOOER010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 6289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6289
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12212_length_6289_cov_7.178697"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="PL162_08650"
     CDS             complement(<1..1077)
                     /locus_tag="PL162_08650"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQF92126.1"
                     /translation="MCLDTRFVLQTLHLSNFRCFSEISLDFHEKLTVFSAPNGGGKTA
                     VLDAVAVALRLFVDTMEGHAASKGFDAWDIRTVRREDGAMEAVPPVRLEAGARMMGED
                     LFWARERASRSASRTTTAEAQGLRRWAEMLSRENTRWVEGKIQTPPDFPLIAYYGTGR
                     LWNFMKLTKGKRTKTPTPNARSRGYTDCLASSSHYRLFADWFRRFSYEATQEQTSGRS
                     SLHRPRELLQAVSETVNAALRPSGWGRLEWDFAEDLPRAHHPVLGQLPVDALSDGIRT
                     MIGLVADLAHRAALLNPHLGRQASRETSGIVLIDEVDMHLHPEWQQQVLPLLLEVFPC
                     VQFLVTTHSPQVLSTVDRRSLRVLR"
     gene            complement(1240..1833)
                     /locus_tag="PL162_08655"
     CDS             complement(1240..1833)
                     /locus_tag="PL162_08655"
                     /inference="COORDINATES: protein motif:HMM:NF017833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HQF92127.1"
                     /translation="MIDHLPNGGVTLAEIRDLVGQDGMLVLTAFLTLVFLVPVSIPGV
                     STVFGAAILLIGICRLGGRNLWLPERIARKTLPTEKLRGALRRGAQWLHSLERLSRPH
                     RMSWMVASGAFPVVNNLALVAGAVLLMAPFGLIPFSNTLPALALLFLSIGLLQRDGLC
                     VLYGHLMNVATVIYFAVLILGGGVALHEIFRHFSQGV"
     gene            complement(2029..>3396)
                     /locus_tag="PL162_08660"
     CDS             complement(2029..>3396)
                     /locus_tag="PL162_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HQF92128.1"
                     /translation="ARGVARRWRWALVLALGMVLGMAPGNGGENPALAATGDRDIADV
                     RTLPQDPALYLPGDPGAFLVPRETTALWEMSFDRAFFSPWEQRGPRGGRDSLLWAFDY
                     LASKDVYGENRRLRSEEWFARQRKNSREEAFGTVNRPGITVEETDLRAFPTEDPAFFS
                     FERAGEGYPFDYLQNSRLKPGEPFFVSHFSEDRRWAFGDAAFASGWVDVRHLAWVDDA
                     SRARWRGAPRAVVLGRDLPLRDLQGEFLAVATTGTLLPLEGSDVLSGLRVRVPRRGEG
                     GRLEYAVARLSFEEATPFPLAFTGWNAARVASALAGEPYGWGGFLNRRDCSAMVRDFF
                     APFGLWLPRNSKAQGASGVVRDVGALDWHAKESLLRAEGRPWRTLVYLPGHIMLYVGS
                     WRGRAVVFHNTWGVRTLSGDREGRHIVGGAVFSTLAPGLELPDLFPEKGILGNRVTSF
                     VLLGE"
     assembly_gap    3397..3496
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3497..5635)
                     /locus_tag="PL162_08665"
     CDS             complement(<3497..5635)
                     /locus_tag="PL162_08665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQF92129.1"
                     /translation="MEDAKGLETQNPSETLLLLGGELALSCQEIALLESAGYRVVRAA
                     SEEQAREEARRTRPDLWLVDLEPGREDEGLHMARRLARELPTALFFRVDLLATGELLN
                     QTEELNPCGYVPRGVGGALLLAALRRGKNVDRARRREEGSFQKDVLGDAVFFRRSLSG
                     VCLVELETDHAGRWVDGRFLDVNASFERQTGLKRETLLGRRASEVLAGVGWEVLLESL
                     ASVTSEETPLRCEQFFSGAGRYFLVTAYPVEPRSLVLVCDDITDRKMREQELARSHLW
                     LESVLRSTGAGFWEWNVQTGETVYSEAWAAMLGYTLQDLGPTTHETWKKLVHPKDAPA
                     SSRLLARHVAGELPYYDCQYRMHHRDGHWVWIHDRGQVATRTEDGKPLMVYGTHMDIS
                     AIKKAEDALREMKNLHEEIVRTMSDGVMLTDDQGRVVFVNREFAALLGEPPKALLGRN
                     WIDFVLPAQRSVAEAADLRRKQGLSDRYELVLQRRDGTRRAVLVGGCARHDKSTGKFL
                     GTMGIFTDITERQRAEREIRKLLEEKDLILQEAHHRMKNHMNTMCSILSLHAQTLEDP
                     KAVRALADAWSRLESMAMLYEMLYRRGAPQAMSLGSYLPELVRSVVALFPQGSRIQVE
                     IAVDEVILSSRTLSALGILVNELVSNAMKYAFAGRNGGVLRVTARQEKGQVYLAVEDD
                     GPGFPLTIDLDRPSGFGLRLVTLLATQLEGT"
BASE COUNT         1250 a         2152 c         1755 g         1032 t
ORIGIN      
        1 gcggagaacg cggagggatc tccggtcgac ggtggaaagt acctgcgggc tgtgggtcgt
       61 cacgagaaac tgcacgcagg gaaagacctc cagcagcaac ggaagcacct gctgctgcca
      121 ttccggatgc aggtgcatgt ccacttcgtc gatgaggacg atgccggagg tctcccggga
      181 agcctgcctc cccagatggg ggttcagcag cgccgcccga tgggcgaggt ccgccaccag
      241 accgatcatg gtgcggatgc cgtcggaaag cgcatcgacg ggaagctgtc ccagaacagg
      301 atgatgcgcc cggggaagat cctcggcgaa atcccactcc aaacggcccc atccagaggg
      361 gcgcaacgcc gcgttcacgg tttcggaaac ggcctgcaag agctccctcg ggcggtggag
      421 agagcttctc cccgaggtct gttcctgcgt cgcctcgtag gagaaccgac ggaaccagtc
      481 cgcgaagagc ctgtagtggg acgatgaggc gaggcaatcc gtgtaccctc ggcttcgggc
      541 gttgggggtg ggggtcttgg tccgcttccc cttggtcagt ttcataaagt tccacaggcg
      601 gccggtgccg taataggcga tcaacgggaa gtccggcggc gtctgaattt ttccttcgac
      661 ccaccgggtg ttttctctgg aaagcatttc ggcccaccgc cgcaaaccct gtgcttcggc
      721 cgtggtggtc cgggaagcgc tccgggaggc ccgttcccgc gcccaaaaga ggtcttcgcc
      781 catcatgcgg gctccagcct ccaggcgcac gggaggaacg gcctccatcg cgccgtcctc
      841 cctacgaacg gtgcggatgt cccaagcgtc gaaacccttg gatgccgcat gtccttccat
      901 cgtgtccacg aagagccgca gtgcgacggc aacggcatca agcacggcgg tcttgccgcc
      961 gccattggga gccgagaaga ccgtcaactt ctcgtggaaa tccaggctga tttccgaaaa
     1021 acagcgaaaa ttggacaaat gcagcgtttg cagaacaaat ctcgtgtcca gacacatgcc
     1081 gttctcacct cctttggagg agcgccccac gccatcacca ggcttcggga tgccctctgc
     1141 aggacaggct tacgtgcctg ccccagctaa ccccgccaga acgcagggcg cctgcgggtt
     1201 ctcgtatccc cataaggaca tctatgaagg caatttccct caaacccctt gggaaaaatg
     1261 ccggaagatt tcgtgcagcg ccactccgcc ccccaggatg agcaccgcaa aatagatcac
     1321 cgtggcgacg ttcatgaggt gaccgtagag cacacagagc ccatcccgct ggagcagccc
     1381 gatggaaaga aagagcagcg ccagggcggg caaggtgttg ctgaaaggaa tgaggccaaa
     1441 gggcgccatg agcaacacgg cccccgccac cagcgccagg ttattcacga caggaaaggc
     1501 accggaggcc accatccagc tcatgcggtg ggggcggctc agccgctcca gcgagtgaag
     1561 ccactgagct ccccggcgta acgccccccg cagtttctcc gtgggaaggg ttttccgggc
     1621 aatacgttcg ggaagccaga gattgcgccc tcccagacgg cagatcccga tgagcagaat
     1681 ggcggcgcca aataccgtgc tcactccggg gatggacacg ggcaccagaa agaccagcgt
     1741 cagaaacgcg gtgagcacca gcatgccatc ctgccccacc aaatcccgaa tttccgccaa
     1801 cgtcaccccc ccgttgggca ggtgatcaat catcccttga atcttttcgc ccagcgacgc
     1861 atcctgggac aaatccttcg aagaggcacc cttttccccg ccacgcaccg catctaccga
     1921 catgtgttca tctcctgact tcccatcctg ggggaatcac cctctccgga gtccctcccg
     1981 atccaaagaa acaccgacgt ccgcagcatg gtcccttctt ccggcccctc actcccccag
     2041 caagacaaag gaagtcaccc tatttcccaa aatccctttc tcgggaaaaa gatcgggaag
     2101 ttccagcccg ggagccagcg tggaaaacac cgctcctccc acgatgtggc gcccctctcg
     2161 gtctcccgag agcgtacgga cgccccacgt gttgtggaac accaccgccc gcccccgcca
     2221 ggagcccaca tagagcatga tgtgccccgg aagatacacc aacgtgcgcc agggacgccc
     2281 ctctgcccgg agaaggctct ccttggcgtg ccagtccagc gcccccacgt cccgcaccac
     2341 cccactggcc ccctgggcct tggagtttct cggaagccag agcccgaagg gggcaaaaaa
     2401 atcccgcacc atggcgctac agtcccggcg gttcaggaag cccccccatc cgtagggctc
     2461 ccccgccaga gccgaagcca cccgggcggc gttccatccg gtgaacgcca gcggaaaagg
     2521 ggtcgcctct tcgaaagaga gacgcgccac ggcatactcc agacgccctc cctctcctcg
     2581 ccggggcacc cgcacccgaa gccccgagag cacgtcactt ccctccagag gcagcaaggt
     2641 ccccgtggtg gccacggcaa gaaactctcc ctggagatcg cgcaggggca gatcacgccc
     2701 cagcaccacc gcccggggag cgccgcgcca ccgggcccgg gaggcgtcat ccacccaggc
     2761 cagatgccgc acgtccaccc accccgaagc aaaggccgcg tcgccaaagg cccatcggcg
     2821 gtcttcggaa aagtgcgaca cgaagaaggg ctccccgggc ttgagccggg agttctggag
     2881 gtagtcaaaa gggtacccct cccccgcccg ctcgaaggag aaaaaggccg ggtcctccgt
     2941 gggaaaagcc cggagatccg tctcttccac cgtgatcccc ggtcggttca ccgtgccgaa
     3001 ggcctcctcc cgggagtttt tccgctgccg ggcgaaccac tcctccgaac gaagacgccg
     3061 gttttctccg taaacatcct ttgaagccag atagtcaaag gcccacagaa gggaatcccg
     3121 ccctccccgg ggtcctcgct gctcccaggg agaaaaaaac gcccggtcaa aactcatctc
     3181 ccagagggcc gtcgtctccc ggggcaccag aaaggccccc ggatcccccg ggagatacag
     3241 ggcgggatcc tggggcaacg tgcgcacgtc ggcgatatcc cgatctcccg ttgccgccag
     3301 ggcggggttt tcccccccgt tccccggggc catgcccagc accataccca gcgccaggac
     3361 cagcgcccac cgccaacgcc tcgccacgcc ccgcgcnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnggta ccttcaagct gggtggccaa aagcgtcacc aagcggagcc
     3541 cgaaccccga cggacggtcc aaatcgatgg tcagaggaaa gccggggccg tcgtcctcca
     3601 ccgccaggta cacctgcccc ttctcctggc gggcagtaac ccgcaacacc ccgccgtttc
     3661 ttccggcaaa agcgtatttc atcgcgttgg acaccagctc attcaccaga atgcccagcg
     3721 ccgaaagcgt tcgggaggag agtatcacct cgtcgacggc gatctccacc tgaatgcggg
     3781 agccctgtgg aaagagcgcc accaccgacc gaaccaactc gggaagatag cttcccagag
     3841 acatggcctg gggagctccg cgccgataga gcatctcgta aagcatcgcc atgctctcca
     3901 gccgactcca ggcgtccgcc agggcccgca ccgccttggg gtcctccaga gtctgggcat
     3961 ggagagagag gatgctacac atggtgttca tgtggttctt catgcggtga tgagcttcct
     4021 gaagaatcag atccttctct tccagaagtt tgcggatttc cctctctgcc cgctggcgct
     4081 cggtgatgtc cgtaaagatt cccatggtgc cgagaaattt ccccgtactc ttgtcgtgcc
     4141 gggcacatcc ccccaccaac accgcccgcc gggtaccgtc cctccgctgt aacaccagct
     4201 cgtatcggtc tgaaagccct tgctttcgcc gcagatctgc cgcttcggcg acagagcgct
     4261 gggcgggcag aacaaagtcg atccaatttc tgccgaggag cgccttcgga ggctccccca
     4321 ggagcgcggc aaactcccga ttcacgaaga ccacgcgccc ctggtcgtcc gtcagcatca
     4381 ccccgtcgga catggtccgg acaatttctt cgtgaaggtt tttcatctca cggagagcgt
     4441 cctccgcttt cttgatggcg gaaatatcca tgtgcgtgcc gtacaccatg aggggtttgc
     4501 cgtcttcggt ccgggtcgcc acctgccccc ggtcgtgaat ccagacccag tgcccatccc
     4561 ggtggtgcat gcggtactgg cagtcgtaat acggcagctc ccccgccacg tggcgggcca
     4621 aaagccggga agaggcgggg gcgtccttgg ggtgaaccaa ttttttccac gtctcgtgcg
     4681 tcgtgggccc cagatcctgg agcgtatacc ccagcatggc ggcccaggcc tcgctgtaca
     4741 ccgtctctcc ggtttgcaca ttccactccc agaacccggc ccccgtgctc cggagaacgc
     4801 tctccagcca aagatgactc cgggccagct cctgttcccg catcttccgg tcggtgatgt
     4861 cgtcgcagac cagcaccagg gatcgaggct ccaccggata ggcggtgaca agaaaatacc
     4921 gccccgcgcc ggaaaaaaat tgctcgcagc ggagcggcgt ttcttccgaa gtcaccgacg
     4981 ctaaggattc cagcagaacc tcccacccca cgccagccag cacttccgac gcccggcgcc
     5041 ccaggagggt ctcccgtttc aggccagttt gacgttcaaa cgatgcattc acatccagaa
     5101 aacgcccatc cacccaccgc cccgcatggt cggtctccag ctccaccaga cacacgccgg
     5161 aaagggagcg gcgaaagaac accgcatctc ccagaacgtc cttttgaaaa gagccttcct
     5221 cccgtcgccg cgcgcgatcc acgttttttc cccgtcgtag cgcggcaaga agaagggctc
     5281 cccccacccc ccggggcacg tatccacagg ggttcagctc ctccgtctga ttgagcagct
     5341 cccccgtcgc caggaggtcc acccggaaga acagcgctgt cggcagctcc cgcgccaaac
     5401 gtcgcgccat gtggagacct tcgtcctccc gcccgggctc cagatccaca agccacagat
     5461 caggccgggt ccgtcgagcc tcctcccggg cctgttcttc cgaggcggcc cgtaccacgc
     5521 ggtatcccgc gctttcgagc agggcgattt cctggcacga cagggccaac tctcccccca
     5581 acaggagcag agtttcactg ggattttgcg tttcaaggcc tttggcgtcc tccatccatg
     5641 ccacctcccc gaagcctgcc agcggcaccg agatcaagac agggccagcg cgttgatctc
     5701 ttctgaggtt ctattatgcc acactctctt cctgaaagtg cggccaaggc ggccgggcat
     5761 ccgcagaggt ccgccgcagg ttatctgccg agggcagaga tgccagcccc catcggggag
     5821 gaggtttttc ggccatggcc acgagcacca ccacgcaacc taaaatgaag taggggcgaa
     5881 aatacgctcg cccctacaga tttccccctg cgctgcattt tccctgtgtc acgagcatgg
     5941 gccagagggg cgcccctacg ggtgtgcgag ccgggagcgc cagcttccag ctggctccct
     6001 tcaatctttt tatctgtgcg tcatcttctg ggctttcgcc cctacggccg tcgcccaatg
     6061 gggagagcct cgtcggcccc agcgacatgg gggcgaaaat acgctcgccc ctacggattt
     6121 caccctacgt cgccagcaga gggcagggat gcaagccacc atggggggcg ttaaataaaa
     6181 aagaaccgat gccggctgga aaacgcgcgc tcccggctat gagctcccag ccgtgcactt
     6241 tcgatcgggc gagatgaact gataacgact ggaagatttt cactcccgg
//
LOCUS       DAOOER010000086         6104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_12610_length_6104_cov_6.766573, whole genome shotgun sequence.
ACCESSION   DAOOER010000086 DAOOER010000000
VERSION     DAOOER010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 6104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6104
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12610_length_6104_cov_6.766573"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(346..1779)
                     /locus_tag="PL162_08670"
     CDS             complement(346..1779)
                     /locus_tag="PL162_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQF92130.1"
                     /translation="MAEAVRQTRVLIVGGGPGGRISYMALRRMGVSSVTLVSNEEPTV
                     ICSLPYGVGRRLVPGGPEEVVVDLAKPSRMPADILKDVLSGTVRSLDAASRRAVVDTA
                     EGPVTVAFETVILAPGAAPWMPPLPGILREIPGPREGTEVVWGDEWVDKGALREHVHV
                     LRGAGDARRLDALASRGGRAVVVGSGAIGLEVAEALVDRGVAVTMAEVLPHVVSALDE
                     ELASRVEARLRERGVTVRTGVRPVEVTSRGLGLQGGELLEGDAVVFATGVRPSVRLAR
                     ELGARVEKGIVVDGQGRTSVAGVLAVGDAVQVEDGATGMPLLPLIGTLAMRQGLVAAA
                     TVVGMPWGLPPATVWGLSEIFGLHWGSVGWTVEAATRAGLPVISLTQPVRTRDPFMPA
                     GHEGVWKLTVAAAEEKGLRRGQILGFQVVQEGDSPLFLAERFIDIITRRETVPELFGH
                     YFIHSPAHNAVDDPYLNLIFQAQALMA"
     gene            1992..2822
                     /locus_tag="PL162_08675"
     CDS             1992..2822
                     /locus_tag="PL162_08675"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HQF92131.1"
                     /translation="MIKSIPLPPHALDCEVLVLSDLHLHSGYEDLGALRTLFERAHPR
                     GVILAGDVANRHALERGALTPERRRAMDDLGGLLVDLRSRAEIIYVRGNHDPGGGILP
                     GLGVVPSYAEIPLEWQGRRFLVTHGHRTVRRSLLRRQIMGSFLTLRRQMRFLSPRGAS
                     ARRIKLSPLARSLERLFARRWLRRARREGYDGVLCGHIHIPELRRLGRHIYANCGDWV
                     DCNTFLALSHRTLFLGSVPPGGRGALLLRQQLPLDPASPPLDPRDPRFDLSSGRWKEG
                     "
     gene            2909..3403
                     /locus_tag="PL162_08680"
     CDS             2909..3403
                     /locus_tag="PL162_08680"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQF92132.1"
                     /translation="MRRMAVVAILAVCCGVFGIGGAWGAPTEQEQQVGSVLDALHDAA
                     SRADGQTYFALFSGDAVFFGTDKTERWPIAEFQAYGQKRFDTGTGWTYKSTERHVYFS
                     ADGKTAWFDERLDNKAGEARGTGVLVKDGDQWKIVQYNLSFPVPNELFDGVYGIIKAY
                     EEKK"
     gene            complement(3526..3840)
                     /locus_tag="PL162_08685"
     CDS             complement(3526..3840)
                     /locus_tag="PL162_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92133.1"
                     /translation="MRQKLKGIVVMALGLCLLSPVGAEATGFGDFEGGLLKIEPPRKL
                     WIYKKNSTTTRCAISIGSSEYVGISDARWVGPELAVYLVEKDGDTVIRLYSSTTTFKK
                     VK"
     gene            complement(3851..5143)
                     /gene="kynU"
                     /locus_tag="PL162_08690"
     CDS             complement(3851..5143)
                     /gene="kynU"
                     /locus_tag="PL162_08690"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HQF92134.1"
                     /translation="MVADFSPDLSYARRRDAQDPLASFRGRFWIPEGMIYMDGNSLGL
                     CPRDGEASLLELLDQWKKMGINGWLDAPIPWFYLPEHLGALMAPLVGAKPEEVVLSAS
                     TTTNLHTLGATFFRPEGKRGRILADELNFPSDLYALRSQLRLAGRDPENDLILVRSSD
                     GLTLDEDDIVAQMDDSVALAILPSVLYRSGQLLDVERLARAGRERGVVVGFDCSHSAG
                     SVPHHFDAWGVDFAFWCSYKHLNGGPGSPAFLYVNERHFGREPGLAGWFGYAKERQFD
                     LAVDFVPQPSAGGWQIGSPHLLSAAPLLGSLALFAEAGLETVREKSLALTAYLMALVD
                     ETLSPAPYHYRVASPRDPRRRGGHVAVEHPTEAWRICQALKARHIVPDFRPERIIRIA
                     PVALYNSFEDVWRVVQALREIIDQKEYEAFPRERGAVS"
     gene            complement(5183..5932)
                     /locus_tag="PL162_08695"
     CDS             complement(5183..5932)
                     /locus_tag="PL162_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92135.1"
                     /translation="MRRWIKGGCLVLVGVLWGALAAQALDFKAIEAKWQKSRELKDDE
                     MSVKISVTWYAGEYIQALFQQQAEKNLWTQSETDKYAYEFLSSLQLDQYIPVMIAIEN
                     TGDKMHMAPFDKQIVLWIGDKRYEPVEYDRRFNFPLLGKIEGLVYFPKFDEKTGKDLL
                     AGASTVKVGFRGSVTPLYSGPGFIDFAFTPGKDNPDAFFSETTKKRMEIDRLLKRLQK
                     LASEKAELEQKLGAVNAELETLHQRMDELQK"
BASE COUNT         1131 a         2031 c         1899 g         1043 t
ORIGIN      
        1 atctggtcgc catgccccca cgcatagtac cacgcccccg ggggaaacga aagggaggga
       61 aaaaacaggg gaagacggag aaaggggcga gcctcaccaa tctcccctct cgcaccaaaa
      121 agctctgagc ccttaccatc actagctttc aacaaaaagt taggccccac gagaagtcct
      181 cctccgggag aaagatcgcc agcgtggccc acattccgca gcaaagcccg ctggaagatg
      241 ggtgcccccg gcagaagaaa gcgaaagtcg ggaaaaaata agcgctccga gccgcaggaa
      301 aggcggatcg gagcgccacc agggactttt tcggggctta cggggctacg ccatgagggc
      361 ctgggcctgg aagatgagat tgaggtatgg atcgtccacg gcattgtggg ccggagagtg
      421 gatgaagtag tggccaaaga gttccggcac cgtctcccgc cgggtgatga tgtcgatgaa
      481 gcgttccgcg aggaagaggg gcgaatcccc ctcctgcacc acctggaagc ccagaatctg
      541 cccccggcgc aatccctttt cctccgccgc cgccacggtg agtttccata ccccctcgtg
      601 acctgcgggc atgaaggggt cccgggtgcg cacgggctgg gtcagggaga tcacaggaag
      661 ccccgcccgg gtggcggcct ccacggtcca tcccacgctt ccccagtgaa gaccaaaaat
      721 ttcactcaaa ccccagactg tggcgggggg gagaccccag ggcatgccca ccaccgtggc
      781 cgccgccacc aggccctgac gcatggccag cgtgccaatg aggggaagga ggggcattcc
      841 cgtggcgccg tcttccacct gcaccgcatc ccccaccgcc agcacgccgg ccacggaggt
      901 ccgcccctgg ccatccacca cgatgccctt ttccacccgg gcccccagct cccgggccag
      961 gcgcaccgaa gggcgcacgc cggtggcaaa gaccaccgca tctccctcga ggagctctcc
     1021 cccctggagc ccgagccccc gggaagtgac ctcgacggga cgcacccccg tgcgcaccgt
     1081 cacgccccgc tcccggagcc gggcttccac ccgggatgcc agctcttcgt ccagggcaga
     1141 caccacgtgg ggcagaactt ccgccatggt caccgccacg ccccgatcca ccagggcttc
     1201 cgccacttcc aggccgatgg cccccgatcc caccaccacg gcccgaccgc ctcggctcgc
     1261 cagggcgtcg aggcgccggg cgtctcccgc accccgaagg acgtggacgt gctcccggag
     1321 ggctcccttg tccacccact cgtctcccca gaccacctcc gtaccctccc gaggaccagg
     1381 aatctcccgg aggatccccg gcaagggggg catccagggc gccgccccgg gggccaggat
     1441 cacggtctcg aaggccaccg tcaccgggcc ttcggcggtg tccaccacgg cccgtcgcga
     1501 cgcggcgtcc agagagcgca ccgttcccga aagaacatcc tttaatatgt cagcgggcat
     1561 ccgggaaggt ttcgccagat ccaccaccac ttcttccggg cccccgggca ccagacggcg
     1621 ccccacccca taggggaggc tacaaattac cgtgggctcc tcgttggaca ccagggtcac
     1681 gctcgaaact cccatgcggc gcaaagccat ataggatatc cgcccccccg ggccccctcc
     1741 cacgatgagc acccgggtct gtcgtaccgc ctctgccatc tttcttcccc ctttcttctt
     1801 ttcgaaccgc tctggatcat ataccccata tgggtatatg tcaaggccct tgggaggcga
     1861 cggagccccc ccgaccttta gaggatggca tcgtcgtctt cgacgctgga caagatttcc
     1921 aaagaagcta tgatgcccga aaggcgatcg gaggacgatt gcgttcctac tcaagaccct
     1981 gggaggcccc cgtgataaag tcgatcccct taccacctca cgccctggac tgcgaagtcc
     2041 tcgtactgag cgacctgcac ctgcactcgg gatacgagga cctgggagct cttcggaccc
     2101 tctttgaacg ggctcacccc cggggagtga tcctcgcggg ggacgtggcc aatcgccatg
     2161 ccctggagcg cggagccctc actccagagc gccgccgggc catggacgac ctgggggggc
     2221 tcttggttga tttgcgatcc cgggcggaaa tcatctacgt ccggggaaac cacgaccccg
     2281 ggggagggat cctgccgggg ctaggagtgg tgccttccta cgctgaaatc cccctggagt
     2341 ggcaggggcg gcgctttttg gtcacccacg ggcaccggac cgtgcgccgc tccctcctcc
     2401 gacgccagat catggggtct tttctcaccc tccggcgcca gatgcgcttt ctctcccccc
     2461 ggggggcttc cgcccggcgg ataaagctct ctcccttggc ccgatccttg gagcggcttt
     2521 tcgcccgacg atggctccgg cgggcccgcc gggagggcta tgacggggtc ctctgcggcc
     2581 acatccacat tccggagctc cggcgcttgg ggcgccacat ctacgccaac tgcggcgact
     2641 gggtggactg caacaccttc ctcgccctct cccaccggac gctttttctg gggtccgtcc
     2701 cccccggagg acggggggcc ttgctcctcc ggcagcagct ccccctggac ccggcttcgc
     2761 cccccctcga ccccagggac ccccgcttcg acctctcctc cgggcgatgg aaagagggct
     2821 agcctcactg cttgacggga aaaatcaagg cgtgttacca tcacggcatc ataataacac
     2881 cgcgagggcg gtgaaaggag tggggtaaat gcggcgcatg gcagtggtgg caattctggc
     2941 ggtatgttgt ggggtcttcg gcatcggagg ggcctgggga gcccccacgg agcaggagca
     3001 gcaggtgggg tcggtcctag acgccctgca cgatgccgct tcccgggcgg atggccagac
     3061 ctattttgcc ctcttttccg gtgacgcggt ctttttcggc acggataaga ccgagcggtg
     3121 gcccatcgcg gagttccagg cctacggcca gaagcgcttc gacaccggca ccgggtggac
     3181 ctacaagtcc acggagcggc acgtgtactt cagcgcagat gggaagaccg catggttcga
     3241 cgagcggctg gacaacaagg cgggcgaggc ccggggtacg ggagtgctgg tgaaagacgg
     3301 cgaccaatgg aagatcgtcc agtacaactt gagcttcccc gtgcccaacg agctctttga
     3361 cggcgtctac ggcatcatca aagcctacga ggagaaaaag tagccccgac gtcggcggaa
     3421 aacacgggga ctagaacgcg aaaggccccc cgaggatcct cggggggcct tcgtttgtcc
     3481 cgtcgccggt ggtcttgggg ccgtcccccc ccccgggccg aggggttact ttactttttt
     3541 aaacgtggtg gtggagctgt agagtcgaat aaccgtgtca ccgtcttttt ccaccagata
     3601 gacggcgagc tcggggccca cccaccgagc gtccgatatg ccgacgtact ccgagctccc
     3661 tatggagatg gcacagcggg tggtggtgga atttttcttg tagatccaca actttctcgg
     3721 gggctcgatt ttcaagaggc ccccctcgaa gtcgccgaag cccgtagcct ccgcccccac
     3781 cggggacaag aggcagagcc ccagggccat tacgacgatg ccttttagtt tttgtctcat
     3841 gaatgctccc ctaggacacg gccccccgct cccgggggaa ggcttcgtat tctttctggt
     3901 cgatgatctc ccgcagggcc tggaccacgc gccacacatc ctcgaaggag ttatagagcg
     3961 ccacgggggc aatgcggatg atgcgctccg gccggaagtc ggggacgatg tgccgggcct
     4021 tcagggcctg gcagatgcgc cacgcctcgg tggggtgctc caccgccacg tgtccccccc
     4081 gtcgccgggg atcccggggg gacgccaccc ggtagtggta tggcgccgga gacagggttt
     4141 catcgacgag agccatgagg taagcggtga gagccaggga tttttccctc accgtctcca
     4201 gccccgcctc ggcaaaaagg gccaaagacc ccagcaaagg ggcggcggac agcaagtgag
     4261 gggaaccgat ctgccaccct ccggcgctgg gctgggggac aaagtccacc gccaggtcga
     4321 actggcgttc cttggcgtag ccgaaccacc ccgccagccc cggctctcgg ccgaaatggc
     4381 gctcgttgac ataaagaaac gccggggacc cgggacctcc gttgaggtgc ttataactgc
     4441 accagaaggc gaaatccacg ccccaggcgt cgaagtgatg gggcacggag cccgcagaat
     4501 ggctacagtc gaagcccacc accacgcccc gctcccggcc cgcccgggcg aggcgctcca
     4561 cgtccaagag ctgaccgctg cgatagagca ccgagggaag gatcgccagc gccacgctgt
     4621 cgtccatctg ggccacgatg tcgtcctcgt ccagcgtcag gccgtccgag ctccggacga
     4681 ggatcaggtc gttttcgggg tctcggcccg ccaggcgcaa ctggctccgc agggcgtaga
     4741 gatccgaagg gaagttgagc tcgtccgcca ggatgcgccc cctcttccct tcggggcgaa
     4801 aaaaagtggc ccccagggtg tgaaggttgg tggtggtaga ggcggaaagg accacctctt
     4861 ccggcttggc ccccaccagg ggcgccatga gggcccccag gtgttccggc agatagaacc
     4921 acgggatggg ggcatcgagc cagccgttga tgcccatctt cttccactgg tccagaagct
     4981 ccagcaggga ggcttctccg tcccggggac agagccccag ggagttgcca tccatgtaaa
     5041 tcatgccctc agggatccag aatcgccccc ggaaagaggc gagggggtcc tgggcatctc
     5101 ggcgtcgcgc gtaggaaaga tcgggagaaa aatcggcgac catgtcgttc cctccttgaa
     5161 tctggcagat aggccgaggg gcctactttt gaagctcgtc catgcgctgg tgcagggtct
     5221 ccagctcggc gttcaccgcc cccagcttct gctccagctc ggccttttcc gaggcgagct
     5281 tctggaggcg cttgaggagg cggtcgatct ccatgcgctt tttggtggtt tcggagaaga
     5341 aggcatcggg attgtccttg ccgggggtga aggcaaagtc gatgaaacct gggccggagt
     5401 agaggggagt cacgctcccc cgaaagccca ctttcaccgt ggaagccccg gccagaaggt
     5461 cttttcccgt tttttcgtcg aacttgggaa agtagaccag gccttcaatt tttcccagga
     5521 gggggaagtt gaaccgtcgg tcgtactcca cgggttcgta gcgcttgtct ccgatccata
     5581 ggacaatctg cttgtcgaag ggagccatgt gcatcttgtc gccggtattt tctatggcaa
     5641 tcatgacggg gatgtactgg tcgagctgca gcgagctcaa aaactcgtag gcgtacttgt
     5701 ccgtttcgga ctgggtccag aggttttttt ccgcctgctg ctggaagagg gcctggatgt
     5761 actcccccgc ataccaggtg acggagatct tgacggacat ttcatcgtct tttagctccc
     5821 gactcttttg ccacttggcc tcgatagctt taaaatccag cgcctgggcg gccagggctc
     5881 cccagagcac tcccacgagc accagacacc ctcccttgat ccatcgcctc atatcttgta
     5941 gcctcccacg ccccaggggc ctcgaatggg gagagctccc tcaaggggct ctcctccggc
     6001 ggcggccaag gccccgccgg agacattttc ccacacccgg gggcgaagct caagggggcg
     6061 gggggatgga aggggcggga ggggatcagg cgggcggacg ggcg
//
LOCUS       DAOOER010000087         5825 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_13345_length_5825_cov_6.949393, whole genome shotgun sequence.
ACCESSION   DAOOER010000087 DAOOER010000000
VERSION     DAOOER010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 5825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5825
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13345_length_5825_cov_6.949393"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2123
                     /locus_tag="PL162_08700"
     CDS             <1..2123
                     /locus_tag="PL162_08700"
                     /inference="COORDINATES: protein motif:HMM:NF014075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HQF92136.1"
                     /translation="WPAILVGAGPSLDKNFSLLHEVKDRAVLIATDAIFKKMLAEGVR
                     PHVVACLEREEMNYTKYFQGIEEDVSDILFVPVTVCVPEMAGTWNGPLMLTHKLGLPL
                     ETWMSQISGLSQLRGGPSVSNVCYSVALALGCRTVALVGQDLAFGPERETHATGAGWD
                     GQTIENFAQLSGKVRAVPGSVEDEVLTDDFFYLVKCTLEETIQFDKKNHEGIEIVDAT
                     EGGARIEGTTVMPLAEFLGTHVTPRDPFPEPPARLLHTCPLDDPGRRSGVREGFAALG
                     ESLELFHKILGDFEAAVDRVDVPVANDAQRRALAFEASRHVDRLMQLSPILTFIIQSH
                     VVEMSGKTVRDRRLATPEEVRRWKTVHREFAQALRSTLERIEEIRSLALALLDRMEED
                     DAFLYEGRERPGGHLTDSRAREVAQRAVEKGDGILAFRLACAYGRFGAEWDPGLLVSL
                     GRLLLASGRVRFARRVFQGIFEAPGREPWLQDATVANDLGVACVSYELGADRDLTNAH
                     YYFSKAYALDPHSEVTEDNLIAFEQILQSSLAVTEGASPGHDFSRAQKTGESMARLEL
                     WEKALGWFEKALTYREVLETLSSDVRGKLLWERALCHEGLGRYAAAEAGYREAARESG
                     DAPEGQWELLRYYLRRHRREEVLEALEALKGLPEGKAFLGFRTPLLRDFGTRHALPWL
                     LDALERICSPQDPSGAQGSGGGCR"
     gene            2257..5088
                     /locus_tag="PL162_08705"
     CDS             2257..5088
                     /locus_tag="PL162_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HQF92137.1"
                     /translation="MRIYHNIPALQTYDALSVTNRALAKSVNKLSTGLRINSAADDAA
                     GLAISEKMRAQIRGLDQAVANSQDGINMIQTAEGALNETHSMLQRMRELSVQAANDTL
                     TSNDRQYIQLEVDQLKEEINRVSNTTQFNKKKLLDGSAAVLWSTDRLETKAIVRGGLR
                     QIDQFGQKNAVEGNFKLKIVADAGEAQIQKTDIFKVKHENVISKVFIHPDSNIENLTV
                     SNLPSGDYKIDVSAAAAIGAGYAGQNTAYMNMAGVLTALELERFSAASAFTTGAVGSF
                     KLEVLSTVAGSSGCYSSMTVKVTWNVAFSTGEGFELSRTTTITVQGTAGDDAEFAITG
                     FAENGNGVNSTLEYQFSVALASAMGTTLAEGAVGWVVFNGKTAMNSYAQILDDYRVNA
                     DVSATAGSLSDFIDLAASNLITKGNNVSQLWEVTEADNQHVKITIKSNYISLSGFAGT
                     DTKEVQFTWTPYTADHLADITISLSTNSDIEDLGGGLGFGHVVLKNASVGFKAGDKVV
                     IESQSLMSKQTRSWAGVDLNMDPNTSWDYGWNPGASSYTSSFSFNQDALDNDTYHFRR
                     FYLNADTGASYDSEVTLDLRGNLGKTDISPAASFHATYVGEVADADVKLRDLDKFWDA
                     NGNFLLENPQAIKIVQGDGTNTSITLYEQDTLDDVAKKLNSAIRDGLGQGKYSGDGQF
                     AQYIDDYTGAAAGPNAVEGTFIVRTAVTGKKGELSFIGDEQLINALSINQIQASKENQ
                     FTVAIEDAHTGVTLKNNLRITGNTLYGELNANVDVEFDAMAGISVGYNSVQNKFLFTN
                     NTSSTAYSTVVHLADNTTVFQIGANEKEDMGINIGAMGTKALGIDSVLVTDRVSAGRA
                     ITTIDAAIDRVSGQRANLGAYQNRLEHTINNLTIASENITSAESRIRDLDMAKEMMNF
                     TRLNILMQAGNSMLAQANQLPQNVLQLLR"
     gene            5172..5555
                     /locus_tag="PL162_08710"
     CDS             5172..5555
                     /locus_tag="PL162_08710"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HQF92138.1"
                     /translation="MAIERVPNITGVEGAGGKNGYGGTPAVVAPELHPKESSLSSPFS
                     REDQEKRPVDEKALQNALSKLEDMSKVFGRHLRFEVKKDPDLVQVSVIDSSTDEVVRK
                     IPPDEVVHMIEQVDRMIGVLFDHSA"
BASE COUNT         1164 a         1809 c         1876 g          976 t
ORIGIN      
        1 ggtggcccgc catcctggtg ggggcggggc cctctctgga caagaacttc tcccttttgc
       61 acgaggtgaa ggaccgggcg gtgctcatcg ccaccgacgc catcttcaag aaaatgctcg
      121 ccgaaggggt ccgtccccac gtggtggcct gcctcgaacg ggaagagatg aactacacga
      181 agtactttca gggcatcgag gaagacgtct ccgacatcct cttcgtcccc gtgacggtct
      241 gtgtgcccga aatggcgggg acctggaacg ggcccctcat gctcacccat aagctgggct
      301 tgcccctgga gacctggatg agccagatct cggggctctc ccagctccgc gggggcccct
      361 cggtatccaa cgtctgttac tccgtggccc tggccctggg gtgtcgcacc gtggccctcg
      421 tgggacagga cctggccttc ggcccggaac gcgaaaccca tgccacgggg gcggggtggg
      481 acggccagac catcgaaaac tttgcccagc tctcggggaa ggtgcgtgcc gttcccggca
      541 gcgtggagga cgaggtcctc acggacgact ttttctatct ggtgaagtgc accctggagg
      601 aaaccatcca gttcgacaag aaaaaccatg aaggcatcga gatcgtcgac gccaccgagg
      661 gaggggcccg catcgagggc accacggtca tgcccctggc ggagttcctg gggacccacg
      721 tgaccccccg ggaccccttt cccgagcccc ccgcccgcct gcttcacacg tgccccctgg
      781 atgaccccgg gcgccggagc ggagtccggg agggattcgc cgcgctggga gaatccctgg
      841 agctcttcca caagatcctc ggggacttcg aggcggcggt ggaccgggtc gatgtccccg
      901 tggcgaacga cgcccagcgc cgggccctgg ccttcgaggc ctcccgccac gtggaccgcc
      961 tcatgcagtt gagccccatc ctcaccttca tcatccagtc ccacgtggtg gagatgtccg
     1021 gaaagacggt gcgggatcgc cgcctcgcca ccccggagga ggtccgccgg tggaaaacgg
     1081 tgcaccggga gttcgcccaa gccctgcggt ccaccctgga gcgcatcgaa gagatccggt
     1141 cgttggccct ggcgctcctg gaccgcatgg aagaggatga cgccttcctc tacgagggga
     1201 gggaacgtcc cggggggcac ctgacggact cccgggcccg ggaagtggcc caaagggccg
     1261 tggagaaagg cgacgggatc ctggcctttc gcctggcctg cgcctacggc cgcttcggcg
     1321 cggagtggga cccggggctt ctggtctccc tggggcgcct tcttctggcc tcggggcggg
     1381 tgcgcttcgc ccgccgggtg ttccagggga tcttcgaagc cccggggcga gagccctggc
     1441 tccaggacgc cactgtggcc aacgacctgg gggtggcctg cgtttcctac gaactggggg
     1501 cagaccggga tctgacgaac gcccactact acttctccaa ggcctatgcc ctggatcccc
     1561 actccgaagt gacggaggac aacctcatcg ccttcgagca gatcctccag tcctccctcg
     1621 ccgtgacgga aggggcgagc cccgggcacg acttctcccg ggcccagaag accggggaat
     1681 ccatggcccg gctggagctg tgggagaagg ccctggggtg gttcgaaaag gccctcacct
     1741 accgggaggt tctggaaacc ctctcctccg acgtccgggg caagctcctc tgggagaggg
     1801 ccctctgcca cgaggggctg gggcggtacg ccgccgccga ggcgggatat cgggaggcgg
     1861 cccgggagag cggcgacgcc cccgaggggc agtgggagct tttgcggtac tatctgcgtc
     1921 gccatcggcg ggaggaggtc ctggaggccc tggaggccct gaagggcctt cccgaaggca
     1981 aggctttctt ggggttccgg acccccctgc tgcgggattt cggcacccgt cacgccttgc
     2041 cgtggctcct cgacgccctg gagcggatct gttcccccca agatccctcc ggggctcaag
     2101 gatccggcgg aggatgccga taacaatatc gatgaggaag ggaggtgggg ctttccgaac
     2161 ggaggacgaa gcgttccccg ggcacgacgc aaaagggcag tgacggcaat atccccaagg
     2221 attggggcct attctctcat ggaggagtga tcagctatgc gtatctatca caatattccc
     2281 gcgttgcaga cctatgacgc cctgtccgtc accaaccggg ccctggccaa gtccgtgaac
     2341 aagctttcca cggggctgcg catcaactcc gccgccgacg acgcggcggg tctggcgatt
     2401 tccgaaaaga tgcgggccca gatccggggc ctggaccagg cggtggcgaa ctcccaggac
     2461 ggcatcaaca tgatccagac cgccgaggga gcgctcaacg agacccattc catgctccag
     2521 cgcatgcgcg agctttccgt gcaggccgcc aacgacaccc tcacctccaa cgaccggcag
     2581 tacatccagc tggaggtgga ccagctcaag gaagagatca accgcgtttc caacaccacc
     2641 cagttcaaca agaagaagct cctggacggt tccgcggcgg tgctctggtc cacggaccgg
     2701 ctggagacca aggccatcgt ccgcgggggg ctgcgacaga tcgaccagtt cggccagaag
     2761 aacgccgtgg aagggaactt caagctcaag atcgtggccg atgcggggga agcccagatc
     2821 cagaagaccg atatcttcaa ggtgaagcac gagaacgtca tctccaaggt cttcatccac
     2881 cccgattcca acattgagaa cctcaccgtg agcaacctcc cgtcggggga ctacaagatc
     2941 gacgtctccg ctgcggcggc cataggcgcg ggctatgcgg ggcagaacac cgcctatatg
     3001 aacatggctg gtgtcctaac ggcactggag ttggagcgtt tctccgccgc aagcgccttt
     3061 actaccggtg cggtgggatc cttcaagttg gaggtccttt ctacagtggc cggaagttcg
     3121 ggatgctaca gtagcatgac tgtgaaggtt acttggaatg tggccttttc tactggagaa
     3181 ggttttgagc tttctcgaac gaccaccatt actgtgcagg gaacagctgg ggacgacgct
     3241 gagtttgcaa taaccggatt cgcagaaaat ggaaatgggg ttaattctac gctggagtac
     3301 caattctcgg tggctcttgc ctctgcgatg ggcacaacgt tggccgaggg cgccgtgggg
     3361 tgggtcgtct tcaacggcaa gaccgccatg aattcctatg cccagatcct ggacgactac
     3421 cgggtgaatg ccgacgtctc cgccaccgcg gggtctctgt cggacttcat tgacctggct
     3481 gcctccaacc tcatcaccaa ggggaacaac gtgagccagc tctgggaggt caccgaggcg
     3541 gacaaccagc acgtgaagat cacgattaag tctaattaca tctctctgtc ggggttcgcg
     3601 gggaccgaca ccaaggaagt ccagttcacc tggaccccct acactgcgga ccatctggcg
     3661 gacatcacca tctctctgtc caccaattcc gacattgagg acctgggggg cggtctgggt
     3721 tttggtcatg tggtactgaa gaacgcttcg gtgggcttca aggcggggga caaggtggtc
     3781 atcgagtccc agtccctcat gagcaagcag acccgctcct gggccggtgt ggacctcaac
     3841 atggatccca acacgtcctg ggactacggc tggaatccgg gggcgagttc gtacacgagc
     3901 tccttctcgt ttaaccagga cgccctggac aacgacacct accacttccg tcggttctac
     3961 ctgaacgccg acaccggggc gtcctacgac agcgaagtga ccttggacct ccggggcaat
     4021 ctgggaaaga cggacatcag tcccgcggcc tccttccacg ccacgtatgt gggagaagta
     4081 gcggatgcgg atgtgaagct ccgggatttg gacaagttct gggacgccaa cgggaacttc
     4141 ctgctggaga acccccaggc catcaagatc gtccaggggg atggcacaaa caccagcatc
     4201 accctctacg agcaggacac cctggacgat gtggcgaaga agctcaatag cgccatccgg
     4261 gatggcctgg gccagggcaa gtacagcggt gacgggcagt tcgcccagta catcgacgac
     4321 tacacggggg cggctgcggg gcccaacgcc gtggaaggca cgttcatcgt ccgtaccgcc
     4381 gtgacgggca agaagggcga gctgagcttc atcggcgacg agcagctcat caatgccctg
     4441 agcatcaacc agatccaggc gtcgaaggaa aaccagttca ccgtggccat tgaggacgcc
     4501 cacaccggcg tcaccctcaa gaacaatctg cggatcacgg ggaacaccct ctacggggag
     4561 ctcaacgcca acgtggatgt ggagttcgac gccatggcgg ggatctccgt ggggtacaac
     4621 tcggtccaga acaagttcct cttcaccaac aacacctcct ccacggccta ctcgaccgtc
     4681 gtccacttgg cggacaacac cacggtcttc cagatcggcg ccaacgagaa ggaagacatg
     4741 gggatcaaca tcggcgccat gggcaccaag gccctgggca tcgacagcgt gttggtgacg
     4801 gaccgggtgt ccgcgggacg cgccatcacc accatcgacg cggccatcga ccgggtctcg
     4861 ggccagcggg ccaacctggg ggcctaccag aaccggttgg agcacaccat caacaacctg
     4921 acgatcgcca gcgagaacat cacctccgcc gagagccgca tccgcgacct cgacatggcc
     4981 aaggaaatga tgaacttcac gaggctcaac atcctcatgc aggcgggcaa cagcatgctg
     5041 gcccaggcca accagctgcc gcagaacgtg ctgcagctgc tccggtagac gcggctttag
     5101 gaactccggg agggaggggg aaaccctccc tccgttccgt tttctaaggc gcgagaaacg
     5161 gagggacggt catggccata gaacgggtac cgaacataac gggcgtggaa ggggccggag
     5221 gcaagaacgg ctacggcggc accccggcgg tggtcgctcc ggagctccat cccaaagagt
     5281 cttctttgtc gtctccgttc tcccgggagg accaggaaaa gcgtcccgtg gacgagaagg
     5341 ccctgcaaaa cgccctgtcc aagttggaag acatgtccaa ggttttcggg aggcatctgc
     5401 ggttcgaggt gaaaaaggat ccggacctgg tgcaggtgag cgtcatcgac tcgtccaccg
     5461 acgaagtggt gcgaaagatt ccgccggacg aggtggtgca catgatcgaa caggtggacc
     5521 ggatgatcgg ggtgcttttc gaccattcgg cgtgagatgg ttctgacagc ggagggagga
     5581 ggggctcggc cccttcctcc cttttttgat ggacgaaacg gcgaagcgag gaaaaggggg
     5641 ggatggggca tggcagagga acggtggaag ggcgcactca tcggacttgg tcagatggcc
     5701 tggaagtatg acggagggcg agcttccgac ccggaggacc cccggacgca cgcctcggcc
     5761 tatggggccc ggggtgacgg cattctcgcc ggggggtgct ctcccgaccc ccgggagcgg
     5821 gaaat
//
LOCUS       DAOOER010000088         5732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_13590_length_5732_cov_8.562269, whole genome shotgun sequence.
ACCESSION   DAOOER010000088 DAOOER010000000
VERSION     DAOOER010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 5732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5732
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13590_length_5732_cov_8.562269"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="PL162_08715"
     CDS             complement(<1..285)
                     /locus_tag="PL162_08715"
                     /inference="COORDINATES: protein motif:HMM:NF014019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HQF92139.1"
                     /translation="MKKNLLVCTTDAPPGGESVECLGTVYATCCLSKSLVADIGANVR
                     NWTIGGELPAYSSLMSEGISAVLDRLQRQGEEVGADAIVGFRLSTTSIAQG"
     gene            complement(282..2618)
                     /locus_tag="PL162_08720"
     CDS             complement(282..2618)
                     /locus_tag="PL162_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92140.1"
                     /translation="MDSSRLIRQALDSGFGPRSIRKKGRLCDAAGLRRIGVLWIFIVF
                     SLCPLGTSGHAESPSPPASADVEAARFLAEGQARARAVEGALAALPSVIQEEKRRIMD
                     HLDEWSQSRLPSLVERHQAQVAQALEGTSLPEDLSTWEVYWRHLKAGVGATEDLEALI
                     RLFMNHAETRLAPAMADFQETLAQELQEEINRLLDEALGRIRKPFRDVTLKYFPFYQG
                     MGLSPPRPRSSLGEIDLLSKDRRGPESALFLVTGLILVAGRKVIQRVLLHVLRSVVGK
                     ALAKLIPLLGWLLLAWEVWDATNAKLSLEDTMRQNFLEEYRGAVTAPTLWSQGEEGSL
                     RTSVEREVEGLLRRFERGCQEEARRMLDVAPALAASDSLRDFVAKGTAQGRDFAVIAE
                     QVKELRELFGEHLLARTPVEVLLELRALAPDLEELAILAREEDRRLIEEYQRSGKAYL
                     EAVHAMGTRTFIELRPLIYALAPSGEGPPPWLTLRRAFTTLVERDKNRAAARGLLILL
                     RVAVPPETFSEASLARVGAREDLFLALYRAVGGKAEILAALFSSEELLAKVARARDGD
                     AEMADALLRQTTDAFWASWAEGDLRDLLAAARVRQELFRLSPDDAVRGIHQTGELALI
                     YRESGRVGVELWYRFVGGEGGDVQKRWAREALSLAARGFDPDKLRTPEDLSWANFCAL
                     LPVGGPSLFDLLSRVGQTARFILVLGALALPLLFVIWVGKRIATLLRDSRRTPPAGAR
                     IYTPTPPPEAVDAVFSEKNAPSPALPKKEETPTKMDEP"
     gene            3426..5117
                     /locus_tag="PL162_08725"
     CDS             3426..5117
                     /locus_tag="PL162_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQF92141.1"
                     /translation="MGKNVVIIGGVACGSKVAARLRRLDPEASITVLERGYFPSYAGC
                     GMPYFLGGVVSDYRHLTSTPVGIERDAAFFRKVKHVDMLTRHEATRIDRERRVVTAIH
                     LESGEERIFPYDVLVLSLGAQPVRPNLPGRDLAGVFSLGTLEDALAISRALEEGTMRR
                     AVVVGSGLIGLEMTEALKNRGVEVSVVELLDRPLGTLLDRDFGALVAQGLERHGVPFF
                     GGERVVELVGEGGRVATVRTDRRDLPADLVLLAVGVRPSVDLAREAGLTLGPSGGIMV
                     DEHLRTNDDRIYAGGDCVEVPHILSGKSVRQPMGSAANRQGRVIADHIAGLPGRYAGV
                     AGTAIARIFDLTAARTGLDEAGAREAGFDPVGVTVVNSDMPHFMPGSALLALRLVADR
                     STRRVLGGQVVGLGRGDKRLDALVASVTGKITVDDLADLDLGYAPPFSTALDPLTHGA
                     NVLRNKLDGLMDSWSPAELLDRKERGEPFLLVDVRTPDEVRLQGRLPFDDQVNIPLGA
                     LWEKGDTLPGDRTLVVFCKISVRGWDAYGILRNKGFGRVVLLEGGTAGWPYGLKG"
     gene            5362..>5732
                     /locus_tag="PL162_08730"
     CDS             5362..>5732
                     /locus_tag="PL162_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HQF92142.1"
                     /translation="MTERKKMSLLYKISMGFVVGVVLGVAVPGVVPTLAPVGKIFISL
                     LKMLIVPLVFSSIVVGTSSLGDVKVLGRVGIKIMVLYLLTTAFAVTLGLFFGNVIQPG
                     AGMELSLTGVEFKMPEVPKMI"
BASE COUNT          930 a         1747 c         2042 g         1013 t
ORIGIN      
        1 cccctgggcg atggaggtgg tggaaaggcg aaatcccacg atggcgtcgg ctcccacctc
       61 ttccccctgg cgctgaaggc ggtccagcac cgccgaaatc ccctcgctca tgagggagct
      121 ataggcggga agctcccctc cgatggtcca gttcctcacg ttggccccga tatcggccac
      181 cagggatttg ctcaggcagc aggtggcgta caccgtgccc aggcactcca cgctctcccc
      241 cccggggggg gcgtccgtgg tgcataccag cagatttttt ttcatggctc atccatttta
      301 gtcggggtct cttctttctt gggcagcgcc ggggaggggg cgtttttttc cgaaaacacg
      361 gcgtccacgg cctccggggg cggtgtgggg gtgtagattc gggcccccgc agggggcgtg
      421 cgccgggagt cccggagcag ggtcgcgatg cgttttccca cccagatcac aaaaagaagg
      481 ggaagggcca gggcccccag gaccaggata aagcgggcgg tctgccccac gcgggagagc
      541 aagtcgaaaa gcgatggccc tcccacgggg agcagggcgc agaagttcgc ccaggatagg
      601 tcttccgggg tgcggagctt gtccgggtcg aatccccggg cggccaggga aagggcctct
      661 cgggcccagc gtttctgcac atcccctccc tctcctccca cgaagcgata ccagagctcc
      721 acccccacgc gccccgactc ccggtagatc agggccagct ctccggtctg gtggatcccc
      781 cgcacggcgt cgtcggggga gaggcgaaag agctcttgtc gcacccgagc ggcggccagg
      841 aggtcccgga ggtctccctc ggcccagctg gcccagaaag catccgtggt ctggcgcaac
      901 agggcgtcgg ccatctcggc gtcgccgtcc cgggctcgcg cgaccttggc gaggagctct
      961 tccgaggaaa acaaggcggc gaggatttcc gcctttcccc ccaccgcccg gtacagggcg
     1021 aggaaaaggt cctcccgggc ccccacccgg gccagggagg cctcggagaa ggtctccggc
     1081 ggcacggcga ccctcaggag gatcaaaagg ccccgggcgg cggcccgatt tttatccctt
     1141 tccaccaggg tggtgaaggc tcgccgcagg gtgagccacg gcggcgggcc ttcaccggag
     1201 ggggctaggg cgtagatcag ggggcggagt tcgatgaaag tcctggtccc catggcgtgc
     1261 accgcctcca ggtaggcctt ccccgaccgt tggtactctt cgatgagcct tcggtcctcc
     1321 tcccgggcca ggatggccag ctcttccagg tcgggggcca gggcccggag ctccagaagc
     1381 acctccacgg gggtccgggc gaggagatgt tccccgaaga gctcccgcag ttccttaacc
     1441 tgctccgcga tcaccgcgaa gtcccgtcct tgggcggtgc ctttggcgac gaagtcccgg
     1501 agggagtcgg aggccgccag cgcaggggcc acgtcgagca tgcgccgggc ctcttcctga
     1561 caccccctct cgaagcgccg gagaaggccc tccacctcgc gttccaccga ggtccggagg
     1621 gagccttcct ctccttggga ccacagggtg ggggccgtga cggcgccccg gtactcctcc
     1681 aggaagtttt gtctcatggt atcctccaga gaaagcttgg cgttggtcgc atcccagacc
     1741 tcccaggcga ggaggagcca ccccaacagg ggaatgagct tggccaaggc cttgcccacc
     1801 acgctgcgga ggacgtggag tagcacgcgc tggatgactt tgcgccccgc caccaagatc
     1861 aaccccgtga cgaggaagag ggcgctttcc gggcctctcc tatccttgct cagaaggtcg
     1921 atctcgccca gggagctccg gggtcgaggg ggtgataggc ccatgccctg atagaaaggg
     1981 aaatatttca gcgtcacgtc ccgaaagggc ttgcggatgc gccccagggc ttcgtccaat
     2041 aggcggttta tctcctcctg gagctcttgg gccagcgttt cctgaaaatc ggccatggcc
     2101 ggggccaggc gggtttcggc gtggttcatg aagagcctta tcagggcctc cagatcctcg
     2161 gtggctccca ctcctgcctt caagtggcgc cagtagacct cccaggtgga gaggtcctcc
     2221 ggcagagagg tcccctccag cgcctgggcc acttgggcct gatgccgttc caccaaggag
     2281 ggaagccggc tttggctcca ctcgtccagg tggtccatga tgcgccgttt ttcctcctgg
     2341 atgacgctgg gaagggccgc cagggccccc tccacggccc gcgcccgggc ctgaccctcg
     2401 gccagaaagc gggcggcctc cacgtccgcg ctggcggggg gcgaggggct ttcggcgtgc
     2461 cccgacgtcc cgagggggca gagggaaaat acgataaaaa tccataaaac cccgatcctc
     2521 cggagcccgg cggcgtcgca gaggcgcccc ttcttccgga tgctccgggg gccgaagccg
     2581 gagtcgaggg cttggcgaat aagacgggaa gagtccatga gtcttcctcc ttgcgttcat
     2641 gataggtcca gggttgcctt cccctcctcg ggagatcccc ggggggaggg ccttcttcgg
     2701 tgccaggctc cgaaaggccc ccgggggacc acggcgcttc tcgccgatcc acaggcggat
     2761 cgatcctaat aaagggcttt cctcggcctg cttcgaaaag gggtgagcgc catggcgacg
     2821 ggacgcgacc ccttgaagaa agatttcctc ggtggagggt ttggctgcgc ctcgcgccac
     2881 cgccttccga aggggcgcag cctttcatct tagcatcgcg gaatatgcct tccccgtcaa
     2941 gggcctttga cgtcttgggg agggccttcg gcatggtcga tcctgggcgg ggggagaaga
     3001 gggcccccgg ggcaaatcgg ggcgacagtt ttgttaaaat agagccacga cgggtcgggc
     3061 ctccaacgaa gcaggcttcg gccgggagga tcgcggcgtc aagggacgag ataaggggtc
     3121 gggtggggtg tcgatcggcc ctcctccccc caggagggac gcctccttaa gggaggcaag
     3181 ggaggaaaat cgatggcggc ccgagggaac ggaaggcccg ggtcttttcg cccggcgatg
     3241 ggagtttttc cggcccgggg ccccggggat ggggcggtct cgccgatgtt tgggaaaagc
     3301 cttgacgggg ggaaaagagt agttttatca tggcttatat gaaaagcttc gtcccgatcc
     3361 cccttcggga ggggtggccg gggcgaaatc tgggggaccc cggcggggtc caggaggatt
     3421 ggaccatggg taaaaacgtg gtgatcatcg gaggcgtggc gtgtggcagc aaggtggcgg
     3481 cgcggttgcg gaggctcgac cccgaggcct ccatcaccgt gctggagcgg gggtattttc
     3541 ccagctacgc ggggtgtggc atgccttact tcctcggcgg cgtggtgtcg gactatcgcc
     3601 acctcaccag cacccccgtc ggcatcgagc gagatgcggc gttttttcgg aaggtgaagc
     3661 atgtggacat gctcacgcgc cacgaggcca cccgtattga ccgggaaagg cgcgtcgtca
     3721 cggcgatcca cctggagagc ggcgaggaac ggatctttcc ctacgatgtg ctggtgctct
     3781 ccctgggggc ccagccggtg cgcccgaacc ttccggggag ggatctggcg ggggtctttt
     3841 ccctgggcac cctggaggat gccctggcga tctcccgggc cctggaggaa gggacgatgc
     3901 gtcgggccgt ggtggtggga tcggggctca tcggcctgga gatgacggag gccctgaaaa
     3961 accgaggggt ggaggtctcc gtggtggagc tcctggaccg tcccctgggt acgcttctgg
     4021 accgggactt cggcgccctg gtggcccaag gattggagcg ccacggggtg cccttcttcg
     4081 gtggcgagcg ggtcgtggag ctcgtgggag aaggggggcg cgtggcgacg gtgcgcaccg
     4141 accggcggga ccttcccgca gatctggtgc tattggcggt gggagtgcgc ccctcggtgg
     4201 acctcgcccg ggaggcgggg ctgaccctgg ggccctcggg gggcattatg gtggacgagc
     4261 accttcgtac caacgacgat aggatctacg cgggggggga ttgcgtggag gtgccccaca
     4321 tcctgtcggg gaaaagcgtg cgccagccca tggggtcggc ggcgaaccgc caggggcgcg
     4381 tcatcgcgga ccacatcgcg ggactcccgg ggcgctatgc gggggtggcg ggtaccgcca
     4441 tcgcccggat cttcgacctc acggcggccc gtaccgggct ggatgaggcg ggggctcggg
     4501 aggcgggatt cgatcccgtg ggagtcaccg tggtgaattc cgacatgccc cacttcatgc
     4561 ccggatcggc cctcctggcg ttgcgcctcg tggcggatcg aagcacccgg cgggtcctcg
     4621 gggggcaggt cgtgggcctc gggcgagggg acaagcgtct ggatgccctg gtggcttcgg
     4681 tgacgggaaa aatcaccgtg gatgacctgg cggacctgga cctgggctat gctcctccct
     4741 tcagcaccgc cctggatcct cttacccacg gggccaatgt cttgcgcaac aaactggatg
     4801 gcctgatgga ctcctggtct ccggcggagc tcttggaccg aaaggaacgg ggggagccct
     4861 tcctgctagt ggacgtgcgc acccccgatg aggtccggct ccaggggcgc ctgcccttcg
     4921 acgaccaggt gaacattccc ctgggggccc tctgggagaa gggagatacc ctgcctggcg
     4981 accggaccct ggtggtcttt tgtaaaattt ccgtccgggg gtgggatgcc tatggcatcc
     5041 tccggaacaa ggggttcggt cgggtggtac tgctggaggg gggcaccgcc gggtggccct
     5101 acggcctcaa gggttaggat cggaccgtcc ggaggaaaag aggttcaagg ggcgagagcc
     5161 ctcggcatgt tccggggtcc cccttgtgcc cgaggccggg tgatgagggg ccaaatgtat
     5221 cactccccgg agcgttcccc cgatccgatc cttgtgctat gttggcaagg cgttgaagag
     5281 gctcccccga aagaataagg gttttagggg ggcagggccg gcggcggggc cgctcgagga
     5341 gaaaaaaggg ggtttgatga cgtgacggag cggaagaaga tgtcgttact ctacaagatc
     5401 agcatgggtt ttgtggtggg cgtggtgctt ggggtggcgg tgcccggcgt ggtgcccacg
     5461 ctggcgccgg tgggcaagat cttcatctct ctcctgaaga tgctcatcgt ccccctggtg
     5521 ttttccagca tcgtggtggg cacttcctcc ctgggggatg tcaaggtcct ggggcgcgtg
     5581 gggatcaaga tcatggtgct ttacctgttg acgacggctt tcgcggtgac gctgggactg
     5641 ttctttggca acgtgatcca gcccggggcg gggatggagc tttccctcac cggcgtggag
     5701 ttcaagatgc ccgaggtccc caagatgatc aa
//
LOCUS       DAOOER010000089         5689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_13724_length_5689_cov_6.809017, whole genome shotgun sequence.
ACCESSION   DAOOER010000089 DAOOER010000000
VERSION     DAOOER010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13724_length_5689_cov_6.809017"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..2277)
                     /locus_tag="PL162_08735"
     CDS             complement(112..2277)
                     /locus_tag="PL162_08735"
                     /inference="COORDINATES: protein motif:HMM:NF021570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HQF92143.1"
                     /translation="MISHLGGQFAVFLGRDLGPLLRWELHLLVLGLVALPLTRRLIPF
                     RLDGGYPFAKALGVILPAYALWLAGSFGLGSFRPLPAWGAVALVGLCSAAVSRARPMA
                     RPLPWKAILAEELVFFAALTAWTFVRGFQPAIEGLEKFMDFGFVNAVLRGEGMPPPDM
                     WLAGESVNYYYFGHATAAFLVALSGVPGPQGYNLMIATLFALTLGGSFSLATHLALAM
                     PGKRGPVPRPGGVKALLSPVLRRALLAGWIAALLVTVGGNLHTFVFAYAHPALKAAGL
                     AEGEVKSYWYPDATRYIGYNPPTNDKTIHEFPFYSFVVADLHGHVSSIPTVLTGGALT
                     LGAFLRGVTPLRLGSMGLLLGIMYMTNAWDFPIYLALWAGALMVRGWKRWGFWKGIAT
                     GVGAGIVAGVVALGAAGPFIASFHNFSQGVFPVMARTPLTQLLVLWGDKLFYSYCFLI
                     FLVAGLARSLSGRPWVAGLSSWWRRVPRGDLFAAGLCLGGILLITAPEVIYVKDIYGA
                     DYHRANTMFKLGYQAFILFGLASGYIVVRIVSSLQGGRRAFFAALFALVVALPLCFPD
                     RAVMGYYNLQNDYKTLDGLAFLQERGHPGDYGAVRWLSENLQGPVPVLEADGDSYTLY
                     GRISMATGLPTVLGWYVHEWLWHNDSQLSANRRGEVATLYNLPGSPEAAALREKYGLR
                     YVVVGALERQRFPQMDEAGLRSLGSVVFSQDQTAIVRID"
     gene            complement(2274..3587)
                     /locus_tag="PL162_08740"
     CDS             complement(2274..3587)
                     /locus_tag="PL162_08740"
                     /inference="COORDINATES: protein motif:HMM:NF005560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQF92144.1"
                     /translation="MKIAIIGAGPTGLVAAHELLKTGASVTVFEREGDVGGLVRMVPS
                     EGEPLEAFYHHIFTSDGAVPELAKEMGFKDRLRWYKPSNALFVRGKLRPFTTPWDLLR
                     LDALSLMDRVRFGLFVLRAQRRQHWRELEGITARQWVEKEAGRAVYEVLWHPLLRSKF
                     GEHADDIGATWLWNKIKLRSSSRGAQQRQEMLGYMDGGFGLLYETLAEDLRRRGGQIR
                     LFCPVERVDRAFEGGLRVTTPEGEERFDRLLATCSPAALASLAPGLPEDFRHRLTALR
                     HMANLCLLMECDASLSPYYWISVAEERCPFVAVVEHTNLLPSSRYGGRHVIYLSRYID
                     PDDPLFQASYSEVESVFLDALEGLFPAWSRDSVRRSRLFRARDAQPVAVRGYDALRPP
                     HATPWPEMTLAAMAQIFPEDRGQNYALRMGRRAPELMGLAGGGAS"
     gene            complement(3584..5236)
                     /locus_tag="PL162_08745"
     CDS             complement(3584..5236)
                     /locus_tag="PL162_08745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQF92145.1"
                     /translation="MTALALAAGIWWRVQGLAWDQGLSYHPDEMNIALAVAKLHIPRQ
                     MHPDFFAYNGFSLYFYRLLAQGAALLTGDPIWTSDLGHITLLGRGCSAVLSVLSLPLL
                     FLLGRRTISPQGGWLALLLGAGCAGLIQAAHYSVTETLLVFWILLQALVAAHMVLSPT
                     PCGKRSWALLGILWGLSLGTKTTAAILLVIPYTVLLLRLRQGDGSRAWAGALGGFLAG
                     CAGALAVSPYSVLDFAAFRHSMEYETSLAKGTLLVPYTLQFLGTTPYLFFLKNIPWHL
                     GPLLPLVSGAGIGGWALAMGRRRSSLAALPLLLFSAVYFAYVGGMFARFIRYLLPLYP
                     ALILAGAWALDALLRWGKARHAPGSVGAWAVPALVGSVVASGLLWGGAVASVFSQENP
                     RTAASRWIYAQIPPGASLVVETWDYRLPAFLPDAPRKPYRHLVVPAPDPDDEAKARLL
                     AEVLSQGDYYILASGRNADQLSRLPERYPLIARFYAQLRSGELGYREIRRFEVFPSLG
                     GFAVDDRGAEETFRVFDHPTVTIYRNEEHRDAKSLQEVLWSP"
BASE COUNT         1121 a         1775 c         2000 g          793 t
ORIGIN      
        1 tgagaaaggg cacgatgacg gaaaacaccg gtgcggccat ggctttctcc tccttattta
       61 tttttgtcga gaaacgcccc gaccccgcag cggggggagc ggtcccttgg ttcaatcgat
      121 gcgcacgatc gccgtctgat cctggctgaa gaccaccgag cctagggatc gcaacccggc
      181 ctcgtccatc tggggaaaac gttggcgttc caacgccccc acgacgacgt agcgcaaccc
      241 gtatttttcc cgcaatgcgg cggcttcggg ggacccagga aggttgtaga gggtggccac
      301 ctccccccga cggtttgccg agagctggga atcgttatgc cagagccatt cgtgcacgta
      361 ccatcccagc acggtgggaa gccccgtggc catggaaatg cgcccataga gggtgtagct
      421 gtcgccgtcg gcctccagca cgggcacggg gccctggaga ttttccgaaa gccatcgcac
      481 cgccccgtag tctcccgggt gtcccctctc ctggaggaag gccaggccgt ccagggtctt
      541 gtagtcgttt tgcaggttgt agtaccccat caccgcccgg tcgggaaagc agaggggtag
      601 ggccaccacc agggcgaaga gggcggcgaa gaaggccctt cgccccccct ggagggagga
      661 gacgatgcgt accacgatgt atcccgaagc caggccgaag aggatgaagg cctggtaccc
      721 cagcttgaac atggtgttgg cccggtggta gtccgcgccg tagatgtctt tgacgtagat
      781 gacctccggc gcggtgatga gcaggatccc ccccaggcag agtccggcgg caaagaggtc
      841 cccccggggg acccggcgcc accacgagga gagccccgcg acccagggcc ttccggaaag
      901 tgatcgggct aaccccgcca ccaggaagat caagaagcag taggagtaaa agagcttatc
      961 cccccagagc accaggagct gggtcagggg cgtgcgggcc atcaccggaa acaccccttg
     1021 ggagaagttg tgaaacgagg cgatgaaggg tcccgccgct cccagggcca ccacgccagc
     1081 gacgatcccc gcccccaccc ccgtggcgat gcctttccag aagccccacc ttttccagcc
     1141 tcgcaccatg agggccccgg cccacagggc gaggtagatg ggaaagtccc aggcgttggt
     1201 catgtacatg atgcccagca ggagccccat gctccccagg cgcagggggg tgaccccccg
     1261 gaggaaggcc cccaaggtga gggctccccc ggtgagcacg gtggggatgc tcgacacgtg
     1321 accgtggagg tcggccacca cgaaggaata aaaggggaac tcgtggatgg tcttgtcgtt
     1381 ggtggggggg ttgtacccga tgtaccgggt ggcgtcggga taccagtagg acttcacctc
     1441 gccctccgcg agccccgcag ccttcagggc ggggtgggcg taggcgaaga cgaaggtgtg
     1501 aaggttgccc cccacggtca ccaggagggc ggcgatccac cccgccaaga gggcccggcg
     1561 aagcaccgga gataaaaggg cctttacacc cccgggacgg gggacggggc cccttttccc
     1621 gggcatggcg agggccagat gggtcgccag ggaaaagctc cctcccaggg tgagggcgaa
     1681 gagggtggcg atcatgaggt tatacccctg gggccccgga acccccgaga gggccaccag
     1741 aaaggcggcg gtggcgtggc cgaagtagta gtaattcacg ctctccccgg cgagccacat
     1801 gtcgggaggg ggcatgccct ccccccgcag gacggcgttg acgaagccaa agtccatgaa
     1861 tttttctagc ccttcgatgg cgggctggaa gccccggacg aaggtccagg cggtgagggc
     1921 ggcgaaaaag actagctcct ccgccaggat ggccttccag ggcaggggac gagccatggg
     1981 gcgggcgcgg gagaccgcag cggagcagag ccccaccagg gccaccgccc cccaggcggg
     2041 gagggggcga aagctcccca ggccgaagct ccccgccagc cacagggcgt aggcggggag
     2101 gatcaccccc agggccttgg cgaaggggta ccccccgtcc aggcgaaagg ggatcagccg
     2161 ccgggtgagg ggcagggcga ccaggcctag caccagcagg tgaagctccc atcgcagcag
     2221 gggcccgagg tctcgcccca gaaagacggc gaattgcccc cccagatggg agatcacgac
     2281 gcccctcctc ccgcgaggcc catgagttcc ggcgcccttc gtcccatgcg gagggcgtag
     2341 ttttgccccc ggtcctcggg gaagatctgg gccatggcgg cgagggtcat ctcgggccag
     2401 ggagtggcgt gggggggcct gagggcgtca tagccccgca ccgccacggg ctgggcgtcc
     2461 cgggcccgga agaggcgact ccggcgcacg gagtctcggg accaggcggg aaagagccct
     2521 tccagcgcgt ccaaaaagac cgactccacc tcggaatagg aggcctggaa gaggggatcg
     2581 tcgggatcga tatagcgaga caggtagatc acgtgccttc cgccgtagcg ggaggaggga
     2641 agcaggttcg tgtgctccac caccgccacg aaggggcatc gctcctcggc cacgctgatc
     2701 cagtaatagg gagagaggga ggcgtcgcac tccatcagga ggcagaggtt tgccatatgc
     2761 cgcagggcgg tgagccgatg gcggaagtcc tccggcagcc cgggggccaa ggaggccagg
     2821 gcggcggggg agcaggtggc caggagccgg tcgaagcgct cctccccctc gggggtggtg
     2881 acccggagcc ccccttcgaa ggcccgatcc acccgctcca ccgggcaaaa gaggcggatt
     2941 tgcccccccc ggcggcgcag atcctcggcc agggtctcgt agaggaggcc aaagcccccg
     3001 tccatatacc ccagcatctc ctggcgctgc tgcgcccccc gggaggagct ccggagcttg
     3061 atcttgttcc agagccatgt ggcgccgatg tcgtcggcgt gttcgccgaa cttggatcgc
     3121 agcaggggat gccagagcac ttcgtagacc gcccgtcccg cctccttttc cacccattgg
     3181 cgggcggtga tcccctctag ctccctccag tgctgccttc gttgggcccg taacacgaaa
     3241 aggccgaaac ggacgcggtc catcagggag agggcgtcca ggcgcagaag gtcccaggga
     3301 gtggtgaagg ggcgcaactt gccccggacg aagagggcgt tggagggctt gtaccaccgc
     3361 aagcgatcct tgaaccccat ctctttcgcc agctcgggca ccgcgccgtc gctggtgaag
     3421 atgtggtgat agaaggcctc cagaggttct ccctccgagg ggaccatgcg caccagcccc
     3481 cccacatccc cttccctttc gaagaccgtg acggaggctc ccgtcttgag cagctcgtgg
     3541 gccgcgacga gccccgtggg gcccgcaccg atgatggcga tcttcatggc gaccaaagca
     3601 cctcctgaag ggacttggcg tccctgtgtt cttcgttgcg gtagatcgtc accgtggggt
     3661 gatcgaagac ccgaaaggtc tcctcggcgc cccgatcgtc cacggcaaag ccccccagag
     3721 aagggaagac ctcaaagcgg cggatctctc gatatcccag ctctcccgaa cgcaactggg
     3781 cgtaaaagcg cgctatgagg ggatagcgct ccggcaggcg ggagagctga tcggcgttgc
     3841 gcccgctggc caggatgtaa taatccccct gggagaggac ttcggccaaa aggcgggcct
     3901 tggcttcgtc gtcgggatcc ggggcgggca ccacgaggtg gcggtagggc ttccgagggg
     3961 cgtcgggaag aaaggccgga agccggtagt cccaagtttc caccaccaag gatgccccgg
     4021 gggggatctg ggcgtagatc caccggctcg ccgccgtgcg gggattttcc tgggagaaga
     4081 ccgaggccac cgcccctccc cacaggagcc ccgatgccac caccgatccc accagggccg
     4141 gcacggccca cgcccccacc gaccccgggg cgtgacgggc ctttccccat cggaggaggg
     4201 cgtccagggc ccaggccccg gcgaggatca gggcggggta gaggggcagg aggtagcgga
     4261 tgaagcgggc gaacatgccc cccacgtagg cgaagtagac tgcggaaaag agcaaaaggg
     4321 gcaaggcggc gagggagctc cggcggcgcc ccatggcgag ggcccacccc ccgatccccg
     4381 cccccgagac caggggcagg aggggcccca ggtgccaggg gatgtttttc agaaaaaaga
     4441 gatagggggt ggtgcccaaa aactggagcg tgtaaggcac caaaagggtc cccttggcca
     4501 aggaggtctc gtactccatg gagtgccgga aggcggcgaa atccagcacc gaatagggag
     4561 ataccgccaa agcccccgcg catcccgcca gaaagccccc cagggccccc gcccaggccc
     4621 gggatccgtc cccctggcgc agccggagga ggagcacggt gtaggggatg accaggagga
     4681 tcgccgccgt ggtcttggtg cccagggaaa gcccccagag gatcccgagg agagcccagg
     4741 atcgcttgcc gcagggggtg ggagagagca ccatgtgggc ggccacgagg gcctggagca
     4801 ggatccaaaa gaccaaaagg gtctcggtca cggagtagtg ggcggcctgg atgagccccg
     4861 cacaccccgc ccccagtagg agggccagcc atcccccctg gggagagatg gtgcggcgcc
     4921 ccaggagaaa gaggagggga agggatagca cggagaggac ggcggaacac cctcgcccca
     4981 ggagggtgat gtgccccagg tcggaggtcc agatggggtc tcccgtcaga agggccgccc
     5041 cctgggcgag aaggcgataa aaatagaggg agaaaccgtt gtaggcaaag aagtccgggt
     5101 gcatctgccg ggggatgtgg agcttggcca ccgccagggc gatgttcatc tcgtcggggt
     5161 gataggacaa cccctggtcc caggcgagcc cctggacacg ccaccagatc cccgccgcca
     5221 gggccagggc agtcaataaa agggcccacg aagggcttcg tggggagggg gtgggagaac
     5281 tcgtcgaagg atccataaaa aaagtatagc agaaatctcc tctgacgggg gggcgttttc
     5341 tctcgctaaa caataaaaga gggtagtgat cccccccgac gacccttggc ccgcgcgact
     5401 cccgggccct cccaggggct tcgcctccgc aagaggggtc cgatcctccg ggggcggcgg
     5461 gttgatttgg cgagttccct atgggctttt cggccggaat gtcatatgct attctgacgt
     5521 ggccaaggcg catgggaggc gtgacgagag aagaaggtca tagagaagat caaagggggg
     5581 gatggcatgt ctcgacaagg ctggctcctg ctgcttgggg cgatcctttg caacgcgggg
     5641 gccaacgtga gcatgaaggc ggggatggcg gggcgagggg ccgtgctgg
//
LOCUS       DAOOER010000090         5539 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_14139_length_5539_cov_6.233771, whole genome shotgun sequence.
ACCESSION   DAOOER010000090 DAOOER010000000
VERSION     DAOOER010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5539
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14139_length_5539_cov_6.233771"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1776
                     /gene="mnmG"
                     /locus_tag="PL162_08750"
     CDS             <1..1776
                     /gene="mnmG"
                     /locus_tag="PL162_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HQF92146.1"
                     /translation="NLNPDNVALMACNPAVGGPAKGHLVREVDALGGEQARAADASTL
                     HLRWLNTSKGAAVRTLRAQCDLRRYHAHMKNVLESCPSLETFQGHVEALESDGQGVRG
                     VRLRTGEVIVSSRVVLAPGTYLGGKVFLGMTSFSSGPLGQVSAPELGDDLRRKGFEVG
                     RLKTGTPPRIHGDSVDWGSLVAQESAREPLSFSFNNPGRVYDGYACYLTRTNAETHAA
                     VQSYMEENSRFFGLIEGPGPRYCPFIEDRVVRFPDREGHQIFLEPVAEGSKEIYMQNF
                     STGLPLEVQRRMLHSLPGCERARILRPAYAIEYDYLPANQLLPWLETKLLKGPFCAGQ
                     INGTSGYEEAAAQGLLAGINAVRTLRGEEPVVLGRHEGYIGVLVDDLVTRAPREPYRM
                     LTSRCEHRLLLRHDNADRRLTPLGRSLGLVGDGAWEGFLRRERSCEEERRRLREWRIL
                     PSPGVNDLLRAAGTTPLEGAVSGEELLRRPEVTFDLLERLAPREDPPEGGVRARVEAE
                     VKYAGYIERQEASVARLSRMEEMRFPEGFDFQGVRGLLAESAEKLSRCRPHTLGQAGR
                     VAGVTPADLQLLWVALARPGHRNDR"
     gene            1773..2258
                     /locus_tag="PL162_08755"
     CDS             1773..2258
                     /locus_tag="PL162_08755"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HQF92147.1"
                     /translation="MKKVPYKPVHDLLWRSLSEGIRRRMRFEDAARHWESIVGPLLGR
                     RSSLVDLEGNRGVVVAESPMGAQELLMRRSAVLKALRERWGLDLEDLTVRVGPLRPLR
                     PRPESPPPREYTPPEEEAVAPVRREIRPLVDDEAVAESLARMWAAYRRRYPDKGDKGM
                     K"
     gene            2383..3471
                     /locus_tag="PL162_08760"
     CDS             2383..3471
                     /locus_tag="PL162_08760"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HQF92148.1"
                     /translation="MKWPELRLGKHSPRYPLIQGGMGVLISGPRLAAAVAQEGGVGTI
                     ASVGVACRSSSYLQGKNYFQANKIALGDAIREARANVPQGVLAVNAMVALTDYDLHIT
                     TACESGIDVLITGAGLPLKLPELTKDFPDVALVPIVSSVRAAQLIVRKWEKSYGRLPD
                     AFVVETPLYAGGHLGATKMEHVTDRAFSLEAVIPELVTVVEKEFRADIPIIAAGGIWS
                     AEDMEAAFNLGARGIQVGTRFACTQEGDASDRFKQAYIDAKEEDVVVIMSPVGIPGRA
                     LRNPFVERYLAGNVESKPCIANCLTFCSYKKERKAFCIAQALIDAYEGRWEEGLFFCG
                     SNVTRCHRMETVPEIFDAFFGADRSSPR"
     gene            3558..3947
                     /locus_tag="PL162_08765"
     CDS             3558..3947
                     /locus_tag="PL162_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II SORL domain-containing protein"
                     /protein_id="HQF92149.1"
                     /translation="MKFTDVMASGDWKGEKHVPVIEAPDRVAPGEGFTVALSVGKEIA
                     HPNTTEHHIRWIRLYFKPENGKVFEVASCEFDVHGESTEGANKGPVFAEPGATVRVKL
                     HQSGTFLASAYCNIHGLWESSCPITVA"
     assembly_gap    3950..4049
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4050..4664
                     /gene="xth"
                     /locus_tag="PL162_08770"
     CDS             <4050..4664
                     /gene="xth"
                     /locus_tag="PL162_08770"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HQF92150.1"
                     /translation="IFRGQKAYNGVAVASRLPMTEVAFGFGDGESPGDEPRLVRCTVG
                     GIKIINSYVPQGKALDHPDYAYKLRFFRRLRALLERDHRPEDPVLWMGDLNVVPTDRD
                     VTHPENKRQHVCVHEGVRMALEDVLSWGLEDVFRRHRPGDGEFSFWDYRVPRALERNI
                     GWRIDHFFATSPLARASTGAFVERELRREERPSDHTAVVVDFSL"
     gene            4661..>5539
                     /locus_tag="PL162_08775"
     CDS             4661..>5539
                     /locus_tag="PL162_08775"
                     /inference="COORDINATES: protein motif:HMM:NF019135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HQF92151.1"
                     /translation="MISSRREREAKGPPRRKIEGLRIWAGALALMALVAGGAEGNLSP
                     APPLFFTHPLHLGMEVYSLLQDRDGFLWIVTAHAGVAKYDGRKVHFLKEGPQGLSTNT
                     VVAALEDRRGRIWIATAGGGLNVYDKDTNSVRVFRHREGDPHSLNSDTFSYKNHGNQV
                     LLEDRAGGLWCGTLRGVSRLESDDRRGVFFTTVPFADGVPRTVYALLEDRRGKIWVGT
                     DRGLSRLDPSRGVIETWTVGSLGELEGKAVFCLLEDRDGNVWAGGDSGLHGLLASGGV
                     RHIPPGDPESGLPDMAI"
BASE COUNT          924 a         1628 c         1945 g          942 t
ORIGIN      
        1 aatttaaatc ccgataatgt agccctcatg gcctgtaacc ccgccgtggg ggggcccgcc
       61 aagggtcatc tggttcggga ggtggacgcc ctgggggggg agcaggcccg ggccgcagat
      121 gcctctacgc tccatctcag gtggctcaac acctccaagg gggcggcggt gcgcaccctt
      181 cgggcccagt gtgacctgag gcgctatcac gcccatatga aaaacgtcct ggagtcctgt
      241 ccttcgttgg agacctttca agggcacgtg gaggccctgg aaagcgacgg ccaaggggtg
      301 cgaggggtga ggctgcgcac cggtgaggtc atcgtctctt cccgggtggt tctggctccg
      361 ggaacctacc tggggggaaa ggtctttctg gggatgactt ctttttcttc cggccccctg
      421 gggcaggtga gcgcccccga gctgggagat gacctgcggc gtaagggttt tgaggtgggg
      481 aggctgaaga ccggcacgcc cccccggatc cacggcgatt cggtggactg ggggtctttg
      541 gtcgcccagg agagcgcccg ggaaccactt tctttttcct ttaacaaccc ggggagggtt
      601 tacgatgggt acgcctgtta cctcacccga accaacgccg agacccatgc ggcggtacaa
      661 tcttacatgg aggaaaattc ccggtttttc ggcctcatcg agggccccgg cccccgctat
      721 tgccccttca tcgaggaccg ggtggtgcgc tttcccgacc gggaggggca tcagatcttc
      781 ctggagcccg tggcggaggg cagtaaggaa atctacatgc agaacttctc cacgggcctc
      841 cccctggagg ttcagcggcg catgctccac tcccttccgg ggtgcgagag ggcccggatc
      901 ctccgtccgg cctacgccat agaatacgac tacctcccgg cgaaccagct tttgccgtgg
      961 ctcgagacca agcttctaaa ggggcctttc tgcgcagggc agatcaacgg cacgtcgggc
     1021 tacgaggagg cggcggccca ggggcttctg gcggggatca acgcggtgcg caccctccgg
     1081 ggagaggagc ccgtggtgtt ggggcgccac gagggataca tcggggtgtt ggtggatgac
     1141 ctggtgaccc gggccccccg ggagccctac cgcatgctta ccagtcgatg cgaacatcgc
     1201 ctgttgctcc ggcacgacaa cgcggaccgt cgtctgacgc ccctggggcg ttccctgggc
     1261 ctcgtgggag atggggcctg ggagggcttt ctccgccggg aaaggtcctg cgaggaggag
     1321 cgtcgtcgcc tgagggagtg gcgtatcctt ccctctccgg gggtcaatga cctcctccgg
     1381 gccgcaggga cgacccccct ggagggggcc gtgagcgggg aggagctctt gcgccggccg
     1441 gaggtgacct tcgacctgct ggagcgcctc gccccccgag aggatccccc cgaggggggg
     1501 gtaagggccc gggtagaggc cgaggtgaag tacgcggggt acatcgagcg ccaggaggcc
     1561 tcggtggccc ggctgtcccg catggaggaa atgcgttttc ccgagggctt cgactttcaa
     1621 ggggtccggg ggctcttggc ggagagcgcg gaaaagctct cccgctgccg tccccacacc
     1681 ctgggtcagg cagggagggt ggcgggggtg accccggcgg acctccagct cctgtgggtg
     1741 gccctggcga ggccggggca tcgaaacgat cgatgaaaaa agttccctat aagccggtcc
     1801 acgatcttct ctggcgctcc ctctccgagg ggatccgacg gcgtatgcgc ttcgaggatg
     1861 cggcccggca ctgggagagc atcgtggggc cccttctggg acggcggagc tccttggtgg
     1921 acctggaggg aaaccgaggg gtggtggtgg cggagagccc catgggggcc caggagcttt
     1981 tgatgcgccg cagcgcggtg ctcaaggccc tccgggagcg gtggggattg gatctggagg
     2041 atctcacggt gcgggtggga ccccttcgtc cgctccggcc gaggccggaa agtcctcctc
     2101 cccgggagta cacccctccg gaggaggagg cggtggcgcc ggtgaggcgg gagatccgcc
     2161 ccctggtgga cgacgaagcg gtggcggaat ctttggcccg gatgtgggcc gcctaccggc
     2221 gaaggtatcc cgacaagggc gacaagggaa tgaaatgatg tataatagac ggaagaggag
     2281 gaccccgccc ggcctccagc gctccgaagg gagcgacaaa aaaacgggat cctcgcgggc
     2341 agagggcgag ggatccgaga gcggagaggg agtgtgtctt acgtgaaatg gcctgaattg
     2401 cgcttgggaa aacatagtcc tcgctacccc ctcattcagg ggggtatggg ggtgctcatc
     2461 tcggggcccc gtctggcggc cgccgtggcc caggaggggg gggtgggcac catcgccagc
     2521 gtgggggtgg cgtgccgctc ttcttcgtat ctccagggga aaaactactt tcaggccaat
     2581 aaaattgccc taggagacgc catccgggag gcccgggcca atgtgcccca aggggtgctg
     2641 gcggtgaacg ccatggtggc cctgacggat tacgatctgc acattaccac ggcgtgcgag
     2701 agcggcatcg acgtgcttat cacgggggcg gggcttcccc tgaagctccc cgagctgaca
     2761 aaggacttcc ccgacgtggc cctggtgccc atcgtgagct ccgtgcgggc ggcccagctc
     2821 atcgtgcgca agtgggagaa gtcctacggg aggcttccgg atgccttcgt ggtggaaacg
     2881 cccctctacg ccggggggca cctgggggcc accaagatgg agcacgtcac cgatcgggcc
     2941 ttttccctgg aggcggtgat ccccgagctg gtgaccgtgg tggaaaagga gttccgggcg
     3001 gatatcccca ttatcgccgc cggagggatc tggagcgccg aggacatgga ggccgcgttc
     3061 aacctggggg cccggggcat ccaggtgggc acccgctttg cctgtaccca ggagggggac
     3121 gccagcgacc gcttcaagca ggcctatatt gacgccaagg aagaggacgt ggtggtgatc
     3181 atgagccccg tggggatccc cgggagggcc ctgcgcaacc ccttcgtgga gcgctacctg
     3241 gcggggaacg tggagagtaa gccctgcatc gccaactgtc tcaccttttg ctcctacaag
     3301 aaggagcgca aggccttttg catcgcccag gccctcatcg acgcctacga agggcgttgg
     3361 gaggaggggc ttttcttctg cggcagcaac gtgacccggt gccatcgcat ggaaacggtg
     3421 ccggagatct ttgacgcctt tttcggcgcg gataggtctt cccctcggta atcccggggg
     3481 gcgttccccc ggcactggcc ccgggggggg gcgaaagggg tgtggccagt ctattacgct
     3541 gggggaggga ttaaaagatg aaatttacag atgtgatggc aagcggcgac tggaagggcg
     3601 aaaagcacgt ccccgtcatc gaggccccgg accgggtggc cccgggggag gggttcactg
     3661 tggccctttc cgtgggaaaa gaaattgccc accctaacac cacggagcac cacatccgtt
     3721 ggatcaggct ctacttcaag cccgaaaatg gcaaggtctt cgaggtggcc tcctgcgagt
     3781 tcgacgtcca cggggaatcc acggaagggg ccaacaaggg gccggtcttc gccgaacccg
     3841 gggccacggt gcgggtgaag ctccatcaat ccggcacgtt tcttgcgtcg gcctactgca
     3901 atatccacgg cctgtgggag agttcctgcc ccatcaccgt ggcctagggn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tctttcgggg gcaaaaggcc tataacggcg
     4081 tggcggtggc gagccgtctg ccgatgacgg aggtggcctt tggcttcggc gatggggagt
     4141 ctccggggga cgagccccgg ctggtgcggt gcaccgtggg ggggataaaa atcatcaaca
     4201 gctacgttcc ccaggggaag gccctggacc accccgatta cgcctacaag ctccgcttct
     4261 tccgacgcct gcgggcgttg ctcgagcgcg accaccgtcc ggaggacccc gtgctctgga
     4321 tgggggatct caacgtggtc cccacggatc gggacgtgac ccatccggaa aacaagaggc
     4381 agcacgtctg tgtccacgag ggggtgcgaa tggccctgga agacgttctc tcctgggggt
     4441 tggaagacgt cttccggcgg caccgcccgg gggatgggga gttctccttc tgggactacc
     4501 gggtgccccg ggcgctggag cgcaacatcg ggtggcgcat cgaccacttc ttcgccactt
     4561 cccccctggc tagggcctcc acgggggcct tcgtggagag ggagctgcgc cgagaggagc
     4621 gcccctcgga ccacaccgcc gtggtggtgg acttttccct atgatctctt cccgtcggga
     4681 aagggaggcg aaggggcctc cgaggagaaa gatcgagggc cttcggatct gggcgggggc
     4741 cctggccctc atggccctgg tcgccggagg ggcggaaggg aacctctccc cggcgccgcc
     4801 cctttttttc acccaccccc ttcacctggg gatggaggtc tactcgctgc tccaagaccg
     4861 ggacggcttc ctgtggatcg tcaccgccca cgcgggcgtg gccaaatacg acggacggaa
     4921 ggtccacttc ctcaaggagg gcccccaggg gctctccacg aacacggtgg tggccgcctt
     4981 ggaggaccga agggggcgga tctggatcgc cacggcggga ggggggctga acgtctacga
     5041 caaggacacg aactccgtgc gggtcttcag gcaccgggag ggggatcccc attcgctgaa
     5101 tagcgatacc ttttcctaca agaaccacgg gaaccaggtg cttttggagg atcgcgccgg
     5161 ggggctgtgg tgcggcaccc tccggggggt gagccgcctc gaaagcgacg accgccgggg
     5221 ggtctttttt accaccgtgc cttttgccga cggcgttccc cggacggtgt acgccctcct
     5281 ggaggaccga agggggaaaa tttgggtggg gaccgaccgg ggcctttctc ggctagatcc
     5341 ctctaggggc gtcattgaaa cgtggaccgt ggggtctttg ggggagctgg agggaaaggc
     5401 ggtcttctgc cttttagaag accgggacgg aaacgtctgg gccggggggg actcagggct
     5461 ccacggcctt ttggcctccg gaggggtgcg tcacatcccc cccggggacc cggagtcggg
     5521 cctccccgac atggcgatc
//
LOCUS       DAOOER010000091         5534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_14151_length_5534_cov_8.442416, whole genome shotgun sequence.
ACCESSION   DAOOER010000091 DAOOER010000000
VERSION     DAOOER010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14151_length_5534_cov_8.442416"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1994
                     /locus_tag="PL162_08780"
     CDS             <1..1994
                     /locus_tag="PL162_08780"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HQF92152.1"
                     /translation="ALTFELQEMVLAGIAARESLEVEMIRDFLPEQLAFLGICSAREG
                     GKIIKVFMGPQLEGLAVSPTLLDGQWHMTPYLAEDGAVLFTFSLPYGDDWILGGFVPN
                     RVFSALDLQMELQYWSLVADSRGHIFMAWNGDHVLLPGSVVPRSFLQSEHLRPDRYTP
                     RQVYVLSRGLPHGLMMVSGIWKDRALLQAARQGLAVAGGGVVLALLLFFPINFGFLRV
                     SRDFQKISSVVRRLGWEIQHIPDPLQAIDAFRRHWEGEDLGTRVGFRNSEDVVRGFQD
                     LLDAVASQGQEVAALYQETSAMQMELEQSNDELRGALERLEELGTFAQTAAEARSTKE
                     ATGALVRSLMAFSGARAGAVTLGKCAGSSMIAYDGDPFLRKGCVEAMEHWGCAPSRGP
                     GIKAGDGVLWQIFPISYLGETLGALCLAETGPGEGVAKRVEGIMKVFIPHMGGIVRSH
                     VLVDEVRHSYHYLALRLQGVSANHHEETGDHLRRIRDYALFLGRRMGLDEETLEDLGT
                     YSMLHDIGKLKVPREILTKPGPLTPQEFEEVMRHTSYGVDLLGNSEWLGMARDICLYH
                     HEKWDGSGYPEGRVGESIPLVARVVGLADVYDALRSPRCYKPAYDHDRVRKIILDGDG
                     RLAPGHFDPALLSIFADEHRTFEEIFAVHFVDDSAFLRA"
     assembly_gap    2134..2233
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2234..2779
                     /locus_tag="PL162_08785"
     CDS             <2234..2779
                     /locus_tag="PL162_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92153.1"
                     /translation="ETVMKDFMANDFVKTEVHGCIKRVEVTGSAWDGEIYTVTGRIKL
                     EDLRRAVTPALPTKPVDRQAPPAPKKGNVTGLVLDVRHLPLIPAMTFRVLDEGGREVY
                     SLNFVDRERFLSSGLCDYQTNINYAQGAPRVADKPLVVKAVRVVEPRNVDIVVPNSAA
                     GKIKGSAYDFRVTCRVTVVKR"
     gene            2784..3959
                     /locus_tag="PL162_08790"
     CDS             2784..3959
                     /locus_tag="PL162_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92154.1"
                     /translation="MTSTVRGALRGALLLCLSWGVLWGAEGRALAQGASPKDIVVEVE
                     GLAPVEKGDKVKARDEAKRHAYREALEKGIGAYVEGITEMKDFMVVKDKVFSQAQGLV
                     TDLKVLDEFVDQEEIYHLKARCTVSARKLDGVLGPVVIDALGNPRVMVLVDEAIEGEK
                     PFLSTVEGEVLRLFEKAGYLLVDPQQAQTLSDTEVELAKSTGDTARLQELARTFQADV
                     IIYGRAQAVAYAKQRVEGVAIAGVRTQVQLKAIITQTAYVLGTQNFEHKDKGTSVQDA
                     AVKGFTASAHEAALALVHKVAYSMVSGSAGGIPGRTVKVLVEGLSFGDARKVKTSLEE
                     TRGTTAVYQRAFRNGKLELDVNTEGTAEDVAVRLEELGLMVGSVTGSTVEASAKKGE"
     gene            3964..4428
                     /locus_tag="PL162_08795"
     CDS             3964..4428
                     /locus_tag="PL162_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92155.1"
                     /translation="MRRPFALFLLTAVALWAWGAPAAEARKLSEGEIRTIWRANGAVP
                     GVQEFQFGVVDWESRTAAVTGRSSPEASTPSGRLLAKRQAMADAQRNLLYLLYELRYG
                     LPERISSIEVEGHVVMGHIDYQGEEGSRYVVEVSLPLHRLLEECVIWKARVK"
     gene            4481..>5534
                     /locus_tag="PL162_08800"
     CDS             4481..>5534
                     /locus_tag="PL162_08800"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HQF92156.1"
                     /translation="MKSVMAVLALASMLWGAGEASAATRVAVVEFQNNATGSSAQDVE
                     AAARAVTDMLTRELTAIPGLSVFERARLDAIAREQRLAASGLVDSTTAVQLGRLAGIQ
                     WIITGAITEVSYKESGGAIPLFGLPGGVAVGEKKASVALDVRAIEVETGEVRMALSER
                     GTADRQMGGIITAYGAYGQSESGGLVLSATYDCVKKVVAAFRNQLQMRDQITAFRVND
                     VRGSRSVRIEAGAVNGGVQAGQLFLVYVEGSPVMGVDGRILGVEKTNLAVLKVTDVQA
                     NWSDCEVVRGSAAEIQRGDFVTIFSGDPKGVKIGSRGLASLGGDSSAGQGGSYGVPPQ
                     PQTPQPQPQTPQPHPPP"
BASE COUNT          957 a         1618 c         1928 g          931 t
ORIGIN      
        1 tggccctcac gttcgagctg caggagatgg tcctcgcggg gatcgcggcc cgggagtccc
       61 tggaagtcga gatgatccgg gactttctgc cggagcaact ggcctttttg gggatctgct
      121 cggcgagaga gggaggaaag atcatcaaag tgtttatggg gccccagctg gaggggttgg
      181 cggtatctcc caccttgctg gatggccagt ggcacatgac gccctatttg gcagaagatg
      241 gcgccgtact ctttaccttc tccctcccct acggcgacga ctggatcctt gggggattcg
      301 ttcccaaccg ggtcttttcc gctctggacc ttcagatgga gctccagtac tggagtttgg
      361 tggcggactc ccggggacat attttcatgg cttggaacgg cgaccacgtc ctgctccccg
      421 ggagcgtggt gccccggagc tttctccaga gcgagcatct gaggcccgac cgctacaccc
      481 cccgccaggt ctatgtcctc agccggggcc tgccccacgg cctgatgatg gtgagcggca
      541 tctggaagga ccgggcccta ctccaggccg ccaggcaggg attggcggtg gccgggggcg
      601 gggtggtgct ggccctcctg ctcttttttc ccatcaactt cggcttcctc cgcgtctccc
      661 gggactttca aaagatttct tccgtggtgc gccgtctggg gtgggagatt caacacatcc
      721 ccgaccccct tcaggccatc gatgccttcc ggcgccactg ggaaggggaa gatctgggga
      781 cccgggtggg ctttcggaac tccgaggacg tggtgcgggg cttccaggac ctcctggatg
      841 cggtggcaag ccaggggcag gaagtggcgg ccctctatca ggagaccagc gccatgcaga
      901 tggaattgga gcagtccaac gacgagctcc ggggagccct ggagcgcctg gaggagctgg
      961 ggacctttgc ccagacggcg gcggaggccc ggtccaccaa ggaggcgacc ggagccctgg
     1021 tgcgttccct catggccttt agcggggccc gggccggggc ggtgactttg ggcaagtgcg
     1081 cgggatcgtc catgatcgcc tacgacgggg accccttcct ccgaaagggg tgcgtcgagg
     1141 cgatggagca ctggggctgc gccccttccc ggggcccggg gatcaaggcg ggagatgggg
     1201 tgctctggca gatcttcccc atctcctacc tgggagagac cttgggggcc ctgtgcctgg
     1261 cggaaacggg gccgggggag ggcgtggcga aacgggtgga agggatcatg aaggtcttca
     1321 tcccccacat gggggggatc gtgagatccc atgtcctggt ggacgaggtg cgccactcct
     1381 atcactacct ggcccttcgc ctccaggggg tgtccgccaa ccaccacgag gagacggggg
     1441 atcacctccg gcgcatccgg gactacgccc tttttctggg gcggcgcatg ggcctcgacg
     1501 aggagaccct ggaagacctg gggacctaca gcatgttgca cgacatcggc aaactcaagg
     1561 ttccccggga gatcctcacc aagcccggcc ccctcactcc ccaggagttc gaggaggtca
     1621 tgcgccacac ctcctacggc gtggacctgc tggggaactc cgaatggctt ggcatggccc
     1681 gggacatctg cctttaccac cacgagaaat gggacggctc ggggtatccc gaggggcgcg
     1741 tgggagaatc catccccctg gtggcccggg tcgtggggtt ggccgacgtc tacgacgccc
     1801 tgcgctcccc tcggtgttac aagcccgcct acgaccacga cagggtgcga aagatcatcc
     1861 tcgatgggga tgggcgcctg gcgccggggc acttcgatcc cgcccttcta tcgatcttcg
     1921 ccgacgaaca tcgtaccttc gaggagatct tcgccgtcca cttcgtggac gacagcgcct
     1981 ttttgcgggc ctgacctatc cgccccaccc catggcgctc ggcttctcga gaaaaaactc
     2041 gcgggacgga agggagaggc aggtgccctt ccgggggggg cgtgctataa taaactcgtt
     2101 gtcgctgtgg cacaacaaca cgaaaagagg tgtnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnngagacgg tcatgaagga cttcatggcc aacgacttcg tcaagaccga
     2281 agtccacggc tgcatcaagc gagtggaagt cacgggttcg gcctgggacg gagagatcta
     2341 cacggtcacc gggcgcatca agctggaaga cctgcgtcgg gccgtcactc cggcccttcc
     2401 caccaaaccc gtggatcggc aggcgccccc ggcccccaag aagggaaacg tcaccgggct
     2461 ggtgctggac gtgcgccatc tgcccctcat ccccgccatg accttccggg tgctggacga
     2521 gggggggcga gaggtctaca gcctcaactt cgtggaccgg gagcgctttc tttcctcggg
     2581 gctctgcgac taccagacca atatcaacta cgcccagggg gctccccggg tggcggacaa
     2641 gcccctggtg gtcaaggccg tgagggtggt ggaaccccgt aatgtggaca tcgtggtgcc
     2701 caacagcgcg gcggggaaga tcaaggggag cgcctacgac ttccgcgtta cctgccgggt
     2761 gaccgtggtc aagcggtgat cgcatgacct cgacggtgcg gggcgccctg cggggggccc
     2821 tcctgctctg cctgtcgtgg ggtgtgcttt ggggtgccga ggggcgtgct ctagcccaag
     2881 gggcctctcc caaagacatc gtggtggagg tggaaggcct ggctcccgtg gagaagggcg
     2941 acaaagtaaa ggcccgggac gaggccaaac gccacgccta ccgagaggcc ctggaaaagg
     3001 gtatcggggc ctatgtggaa ggcatcaccg aaatgaagga ctttatggtg gtgaaggaca
     3061 aggtcttttc ccaggcccag ggactggtga cggatctcaa ggtgttggat gaattcgtcg
     3121 accaggagga gatctaccac ctgaaggccc ggtgcacggt ctccgcccga aagctcgacg
     3181 gggtgctggg ccctgtggtc atcgacgccc tggggaaccc ccgggtgatg gtgctggtgg
     3241 acgaggccat cgagggggaa aagcccttcc tatccacggt ggaaggggag gtgcttcggc
     3301 tcttcgagaa ggcgggctac ctgttggtgg acccccagca ggcccagacg ctgtcggaca
     3361 cggaagtgga gctcgccaaa tccacggggg acaccgcccg gctccaggag ctggcccgga
     3421 ccttccaggc cgacgtcatc atctacggtc gggcccaggc ggtggcctac gccaagcagc
     3481 gggtggaggg ggtggccatc gcgggggtgc gcacccaggt gcaactcaag gccatcatca
     3541 cccagacggc ctacgtcctg ggcacccaga acttcgagca caaggacaag ggcacctcgg
     3601 tccaggatgc ggcggtgaag ggcttcaccg cttcggccca cgaggcggcc ctggcgctgg
     3661 tgcacaaggt ggcctactcc atggtgagtg gctccgccgg gggcattccg ggaaggacgg
     3721 tcaaggtttt ggtggaaggg ctctccttcg gcgatgcccg caaggtaaaa accagcctgg
     3781 aagagacccg gggcaccacg gcggtctacc agagggcctt ccggaacggc aaattggagc
     3841 tggacgtgaa caccgaaggg acagcggagg acgtggccgt gcgcctggaa gagctggggc
     3901 tcatggtggg atcggtgacc ggaagcaccg tcgaggcctc ggcgaagaag ggagaatgat
     3961 gccgtgagaa gaccctttgc cctgtttctt ctgacggcgg tggccctgtg ggcctgggga
     4021 gctccggcgg cggaggcccg caaactctcc gagggggaaa tccgcaccat ctggcgcgcc
     4081 aacggcgcgg tgccaggggt acaggagttt cagttcggcg tggtggattg ggaaagccgc
     4141 acggcggcgg tgacggggag gtcttccccc gaagcatcca ctccctcggg aaggcttttg
     4201 gccaagcgcc aggccatggc ggatgcccag agaaatttgc tttaccttct gtacgagctt
     4261 cgctacggtc tgccggagcg catctcctcc atcgaagtgg agggtcacgt ggtgatgggg
     4321 cacatcgatt accaggggga agagggcagt cgctacgtgg tggaggtttc cctccccctg
     4381 caccgcctgc tggaggagtg cgtcatctgg aaggcccggg tcaagtgacg tcggcaatct
     4441 cgaacttcgg aggtgcgtgg ggagaatggg agagatgaga atgaagtcgg tgatggccgt
     4501 tctggcactg gcgtcgatgc tgtggggagc cggggaggcg tcggcggcga cacgggtggc
     4561 ggtggtggag ttccaaaaca acgccacggg gagctctgcc caggatgtag aggctgcggc
     4621 tcgggccgtg acggacatgc tcacccggga gctcacggcc attccgggac tttcggtctt
     4681 tgagcgggct cgcctggacg ccatcgcccg ggagcagcgc ctggcggcct cggggctcgt
     4741 ggattccacc accgctgtgc aattggggcg cctggcggga atccaatgga tcatcaccgg
     4801 ggccattacc gaggtgtcct acaaggaaag cgggggggcc atccccctct ttggcctccc
     4861 cgggggcgtt gctgtggggg agaagaaggc ttcggtggcc ctggacgtcc gggccatcga
     4921 ggtggagacc ggcgaggtgc gcatggccct gagcgagcgg ggcacggcgg accgacagat
     4981 ggggggtatc atcaccgcct acggagccta cggtcagtcg gaaagcggcg ggctggtcct
     5041 ttcggccacc tacgactgtg tcaaaaaagt ggtggcggcc ttccggaacc aacttcagat
     5101 gcgagaccag atcaccgcct ttcgggtcaa cgacgtgcgg gggagccggt cggttcgcat
     5161 cgaggcgggg gccgtgaacg gtggcgtcca ggcgggacag ctctttttgg tctacgtgga
     5221 aggatccccc gtgatggggg tggatgggcg catcctcggg gtggaaaaga ccaacctggc
     5281 ggtgctcaag gtgacggacg tccaggcgaa ttggtcggac tgcgaggtgg tgcggggcag
     5341 tgccgccgag atccagcggg gagacttcgt cacgatcttt agcggcgatc ccaagggagt
     5401 caagatcggc agccgggggc tggcgtccct ggggggcgat tcctctgcgg ggcagggggg
     5461 gagctatgga gtgccgcccc agccccaaac tccccagccc cagccccaaa ctccccagcc
     5521 ccaccccccg cccc
//
LOCUS       DAOOER010000092         5521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_14195_length_5521_cov_7.685876, whole genome shotgun sequence.
ACCESSION   DAOOER010000092 DAOOER010000000
VERSION     DAOOER010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14195_length_5521_cov_7.685876"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            265..>1077
                     /locus_tag="PL162_08805"
     CDS             265..>1077
                     /locus_tag="PL162_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HQF92157.1"
                     /translation="MSERNTGLVRNALVMMMGTATSRVLGLVREMMVAALFGATRQLD
                     AFNIAYTIANLARQLLAEGALSAAFVPVFSRTLKDKGKDQAALLARRATFALLALGGG
                     AVLLGLLAAPLLVRILAWGFGPDEQSLAVELTRSMFPYLLIVSLSALAMGVLNSVNCF
                     FVPAVAPALSNVTFIAVLALSAQRWGLWALPGAVLLGGLMHCALQWVWAWRRGIPLVP
                     ALPSRNDEDLRKMMALFLPYAAGLSLNQLHPVISRLFGSFLEGGSISALNYAD"
     assembly_gap    1078..1177
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1178..1560
                     /locus_tag="PL162_08810"
     CDS             <1178..1560
                     /locus_tag="PL162_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HQF92158.1"
                     /translation="VMSALLLPLFSSRGLALASSGAFTVSAVVGYHRVVRHLGQGVGI
                     FSLPWVVRMGWGLLGTLAALLLWRQGWPYPLESSVGWRGAWLGGAAILGGGTYLGIMR
                     SLGGPEWQWLRAAASRRRDEEGLK"
     gene            1557..2099
                     /locus_tag="PL162_08815"
     CDS             1557..2099
                     /locus_tag="PL162_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92159.1"
                     /translation="MKNAWGLRVLGGLLLVCLMGGGVLARGKSLEELLSLRTAVIWVE
                     GQRLGDLVVGARARLEILLVDRDLVEEIYGENSQAPDWLRLHAQYASSEALKGKTFFI
                     VRYKALIPWSFDPQEISVGDHRVDRKDILTRKEFFPSGELPSGMMGELALAVPGSFNK
                     ASEITVAYGEWPSTLKIPGR"
     gene            2275..3348
                     /gene="tsaD"
                     /locus_tag="PL162_08820"
     CDS             2275..3348
                     /gene="tsaD"
                     /locus_tag="PL162_08820"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HQF92160.1"
                     /translation="MIVRSEGVQASPFRPSAPDFLTLGVETSCDDTALCVLAGGRRVL
                     ASVLSSQVRDHAPFGGVIPELASRKHQEAFLPLLEEVLRRAEVRNPPRELDLVAVTMG
                     PGLMGSLIVGVMGAKALAQAWELPLVGVNHLEGHLFANVVAHEDLVFPFLALIVSGGH
                     TEIILARAPGDYALLGATRDDAAGEAYDKVAKLLGMGYPGGPEVDRLARRGDPLRFPF
                     PTPMASSDEISFSFSGLKTAVLWETRRWAAAGKDLPREDLCASFQRAVVDSLMAKVRL
                     AVKRTGVRRVTLSGGVGANSALRRAMESERSFRSYVPPLELCTDNAVMIAAAGYAAYR
                     RGYRCDLTLSPDPVLPLTPRHQE"
     gene            3713..4000
                     /gene="groES"
                     /locus_tag="PL162_08825"
     CDS             3713..4000
                     /gene="groES"
                     /locus_tag="PL162_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HQF92161.1"
                     /translation="MKLKPLGDRIVVKVLSQEEKTKGGIVLPDTAKEKPQEGEVKAVG
                     TGRVLDNGQKLPLDVSVGDRVLFSKYGGTEVKVDGDEFLILSERDVLAIVN"
     gene            4149..>5521
                     /gene="groL"
                     /locus_tag="PL162_08830"
     CDS             4149..>5521
                     /gene="groL"
                     /locus_tag="PL162_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806319.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HQF92162.1"
                     /translation="MAKMIVYGEEARRALERGMNQVADTVGVTLGPKGRNAVLEKKFG
                     SPTITNDGVTIAKEIELADPFENMGAQLLKEVASKTNDVAGDGTTTATVLARAIIREG
                     MKNVAAGANGILMRHGIEKAVDVVVDELKAMSIQVKDRSKIAQVAAISANDKGVGELI
                     AEAMEKVGEEGVITVEDSQTVGSTLEMVEGLQFDKGYISPYMVTDPERMEAALDDAFI
                     LINDGKINNVKDMLPVLEKAVQAGKPLMIIAEDVEGEALATLVVNKIRGILQVVAVKA
                     PGFGDRRKAMLQDIAIVTGGQVVSEELGIKLENVDLAMLGRAKKIRVNKEETTIVEGA
                     GNSDEIRKRAAQIKKEIEDSTSDYDKEKLQERLAKLVGGVAVIQVGAATETEQKELKH
                     RIEDALNATRAAVQEGIVAGGGVALINTLPILNSFVEKLSGDEKTGANAVRRALTEPL
                     HLISSNAG"
BASE COUNT         1027 a         1517 c         1798 g         1079 t
ORIGIN      
        1 ggcgtcaagg gggcctcctg actttttgtc aaagatgtcg tccacgccaa ggactccttt
       61 ctcggttctt gcgggataaa ccccaactcc cctgcggcct cgcgagggag gcccttccgg
      121 cggggggaag aaacggtccg ccgaacgaag ctccgctttt ttgccggggg ccctgcggga
      181 cgggtcctcc accgccgggg gagaggggcg gagctaggtc gtctccgacc gccgggccca
      241 tcgatgggag ggtaccagga aaatatgtcc gaacgcaata ccggccttgt ccgaaatgcc
      301 cttgtcatga tgatggggac ggccacgagt cgcgtcctgg ggcttgtgcg cgagatgatg
      361 gtggccgccc ttttcggggc cacccgccag cttgacgcct ttaacatcgc ctacaccatc
      421 gccaaccttg cccggcaact cttggccgaa ggggcccttt cggcagcctt cgttccggtc
      481 ttttcccgca ctttgaagga caaggggaag gatcaggccg ccctcttggc gcgtcgggcc
      541 acctttgccc ttttggccct ggggggaggg gctgtgctcc tggggctcct ggcggccccc
      601 cttttggtgc ggatcctcgc ctggggtttt ggccccgatg agcaatctct ggcggtggag
      661 ttgacccgga gcatgttccc ctacctgctc atagtatccc tttcggccct ggccatgggg
      721 gtactcaaca gcgtaaactg cttttttgtc cccgccgtgg cccccgccct gagcaacgtg
      781 acgttcatcg cggttttggc cctctcggcc cagaggtggg ggctatgggc cctgccgggg
      841 gcggtccttt tggggggact catgcattgc gccctccagt gggtttgggc gtggcggcgg
      901 ggaatccccc tggtccccgc ccttccttcc cgaaatgatg aagatctgcg aaaaatgatg
      961 gccctttttc taccctatgc ggcggggcta tcgctgaacc agctccatcc ggtcatcagt
     1021 cgcctcttcg gatctttttt ggagggggga tcgatctcgg ccctgaacta cgccgatnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntcg tcatgagcgc ccttctcctg
     1201 ccccttttct cctcccgggg cctcgccctg gcctcttccg gcgcatttac cgtgtcggcg
     1261 gtggtggggt atcatcgagt ggtgcgccac ctggggcagg gggtgggaat tttttccctt
     1321 ccctgggtgg tgcgcatggg atggggtctt ttggggaccc tggcggcgct cctcctgtgg
     1381 cgtcaagggt ggccctatcc cctggaaagt tccgtggggt ggcgcggcgc gtggctcgga
     1441 ggggccgcga tcctgggcgg agggacgtat ctgggaatca tgcgatccct ggggggaccg
     1501 gagtggcagt ggttgcgtgc ggcggcgagt cgccgtaggg atgaggaggg attgaagtga
     1561 aaaacgcgtg gggactaagg gtgctgggag ggttgctttt ggtgtgtctg atgggaggag
     1621 gagtcctggc ccggggtaaa tccctggaag aacttctttc cctccggacc gccgtcatct
     1681 gggtggaggg gcagcgcctc ggagatctcg tggtgggggc ccgggcccgg ctggagatct
     1741 tgttggtgga tcgggatctg gtggaagaaa tttacggaga aaactcccaa gcgccggact
     1801 ggctccggct ccacgctcag tacgcctcgt cagaggcctt gaagggcaag acctttttta
     1861 tcgtccggta caaggccttg atcccctggt cctttgaccc ccaggagatc tctgtgggag
     1921 atcatcgggt ggatcggaag gacatactga cgaggaagga gtttttcccg tcgggggagt
     1981 tgccctcggg tatgatgggg gagttggccc tggccgtccc ggggagtttc aacaaagcat
     2041 cggaaataac ggtggcttac ggcgagtggc catcgacact gaagatacct gggaggtaag
     2101 tacgatgcat cggtatcgct gcgcttcctg cggggcacgc tttaccagtg aaaaacatga
     2161 atctcgttgt cctgagtgcc gaggaaaggt tctcatccac gaagaagggg aaccccgacg
     2221 caagaagagt tgttgctcct ccggatcctg tgccagttgt ggatgcgggt gccattgatc
     2281 gtgagatccg aaggggttca ggcttcgccc ttccgccctt ccgcccccga tttcctcacg
     2341 ttgggcgtgg agacgagttg tgacgacacg gctctctgtg tgctggccgg gggacgccgg
     2401 gtcttggcca gcgtcctctc gagccaggta cgggatcacg ccccctttgg cggagtgatc
     2461 cccgagctgg cgtctcgtaa gcaccaggag gccttccttc cgctcctcga agaggtgcta
     2521 cgtcgggcgg aggtgcgtaa tcccccccga gagctcgacc tcgtggcggt gaccatgggg
     2581 ccggggctca tgggatccct gatcgtgggg gtcatgggag ccaaggcctt ggcccaggcc
     2641 tgggagctcc ccctggtggg ggtgaatcac ttggaagggc atctctttgc caacgtggtg
     2701 gcccacgaag accttgtttt tccgttcctc gccctcattg tctccggagg gcacacggag
     2761 atcattttgg cccgggcgcc gggagattac gccctactgg gggcaacccg agatgatgcc
     2821 gcaggggagg cctacgacaa ggtggctaag cttttgggga tgggttatcc gggcggcccg
     2881 gaggtggacc gccttgctcg aaggggagac ccccttcgct ttccctttcc gacgcccatg
     2941 gcctcgtccg atgaaatatc tttcagcttt agcggtctaa agaccgccgt gctttgggaa
     3001 acgcgccgat gggcggcggc ggggaaagat cttccccgag aggacctctg tgcctcgttc
     3061 cagcgggcgg tggtggactc cttgatggcc aaggtgcgtt tggcggtgaa gcgtacgggg
     3121 gtgcgccgcg tcaccctttc gggaggagta ggggccaata gtgccctgcg ccgggcgatg
     3181 gaaagcgaac ggagcttccg gtcctatgta ccccctttgg agctatgcac ggataacgcg
     3241 gtaatgatag ccgcagcggg gtatgccgcc taccgtcgag gctaccgctg cgacctgacc
     3301 ctctcccccg acccggtgct ccccctaacc cctagacacc aggagtagcc cgctcctttc
     3361 gaggtgtcta aagccgtacg ggtaaaagac atcctctatc gccccgcccc ttggacgaat
     3421 cctgttgagc cgaggtggtg gggccttggg gagcgcgcaa aaagagatta ggacttgccc
     3481 tggtcggcct tcgtcttagg ttccggaggg ccctgtgttt tttggaaaaa caagatccgg
     3541 aaagataata aggtaaaaga agagattttc tcgaaaaaaa accgtgtttt tgggtatatt
     3601 tggagatagc cttccttgag tgatggaggg ctcctcggta agatcagtct cggtcaaatt
     3661 agcactcgtg accgatgagt gctaacacga aaatccttga ggagggatcc tgatgaaact
     3721 gaagcccctt ggagatcgga tcgttgtgaa ggtgctttcc caggaggaaa agactaaggg
     3781 cggcatcgtg ctgcccgata cggccaagga aaagccccag gagggcgaag tgaaggccgt
     3841 gggaacggga cgagtgctgg acaacggcca gaagctcccc ctggacgtca gcgtggggga
     3901 ccgggtgctt ttcagcaagt acggtggcac cgaggtcaaa gtcgacggcg acgaattcct
     3961 tatcctgagc gagcgcgacg tcctggctat cgtcaactag cattataata aaccgcaacg
     4021 acgccttagg ggcggctctc ccagggcccc agagctccct ttggcaccac gaaaagggcg
     4081 gttgggggcg acggacccgc tagagttccc tttgaatgag ataaaaatta ggaggtgctc
     4141 ctgttattat ggctaaaatg attgtgtacg gagaagaagc tcgtcgcgct ctggagcgtg
     4201 gcatgaacca agtggcggac accgtgggcg tgaccctggg ccccaaggga cggaatgcgg
     4261 tcctagagaa gaagtttggc tcccccacga ttaccaacga cggcgtgacc attgccaagg
     4321 aaattgagct cgctgacccc ttcgagaata tgggggctca gctccttaag gaagtggctt
     4381 ccaagaccaa cgatgtggcg ggcgacggca ccaccaccgc cacggttttg gctcgagcca
     4441 tcatccgcga aggcatgaag aacgtggcgg cgggagcgaa cggcattctc atgcgccatg
     4501 gcattgagaa ggccgtggac gtggtggtgg atgagcttaa ggccatgtcc atccaggtaa
     4561 aagaccgcag caagatcgcc caggtggcgg cgatttccgc caacgacaag ggcgtgggtg
     4621 agctcatcgc cgaggccatg gagaaggtgg gcgaagaagg cgtcatcacc gtggaagaca
     4681 gccagacggt gggtagcacc ctagagatgg tggaggggct gcagttcgac aagggctaca
     4741 tcagccccta catggtgacg gaccctgagc gcatggaggc agccctggat gacgccttca
     4801 tcctcatcaa cgatggaaag atcaacaacg tcaaggacat gctccccgtg ctggagaagg
     4861 ccgttcaggc gggcaaaccc ctcatgatca tcgccgaaga tgtggagggc gaggccctgg
     4921 ccaccctggt ggtgaacaag atccgcggca tccttcaggt ggtggcggtg aaagctccgg
     4981 gcttcggcga tcgccgcaag gccatgctcc aggatattgc catcgtcact ggcggccagg
     5041 tggtgagcga agagctgggc atcaaactgg agaatgtgga cctggccatg ctgggtcggg
     5101 cgaagaagat tcgagtgaac aaggaagaga ccaccatcgt tgaaggcgcg gggaattctg
     5161 acgagatccg caagcgcgcc gcgcagatca aaaaagagat tgaggactcc acctccgatt
     5221 acgacaagga gaagctccag gagcgcctgg ctaagctcgt gggaggcgtg gcggtcatcc
     5281 aggtgggggc ggccaccgag accgagcaga aagagctaaa acaccgcatc gaagacgccc
     5341 tcaacgctac ccgggcggcg gtgcaggaag gtatcgtggc cggaggcggc gtggccctga
     5401 tcaacaccct ccccatcctt aactccttcg tggagaagct ttcgggcgac gaaaagaccg
     5461 gcgccaacgc cgtacggaga gccctcaccg agcccctgca cctcatttcc tccaatgcgg
     5521 g
//
LOCUS       DAOOER010000093         5480 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_14326_length_5480_cov_8.167558, whole genome shotgun sequence.
ACCESSION   DAOOER010000093 DAOOER010000000
VERSION     DAOOER010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14326_length_5480_cov_8.167558"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1267)
                     /locus_tag="PL162_08835"
     CDS             complement(<1..1267)
                     /locus_tag="PL162_08835"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HQF92163.1"
                     /translation="MIFSENALTILKKRYLKGDEPPEALLRRVARAVASVEAEGDRSL
                     WEDRFFDLMARLDFLPNSPTLMNAGTPQGQLAACFVLPVGDSMEEIFATLKNTALIHK
                     SGGGTGFNFSSLRPAGDVVSSTNGVASGPVSFMGMFDLATDVVKQGGTRRGANMGILD
                     GDHPDVLDFIRAKTEEGKLANFNISVGLSDDFWRTLEQDGEWTLKNPRTGAPVRTLPA
                     REIFDRIVHCAWATGDPGVIFSDRLNRDNPTPHLGPIVSTNPCGEQPLLPYEACNLGS
                     VNLAHMVTPERAVDGARLEEAVRLGVRFLDNVIDVNSYPLPEIRDMSQGNRKVGLGVM
                     GWAEMLFRMGIPYDSEGALALGGRVMAQIQSVGHDESRRLAQERGAYPNCRGEVRRNA
                     TVTTIAPTGTISLLAGCSSGIEPVFALVHT"
     gene            1577..2869
                     /locus_tag="PL162_08840"
     CDS             1577..2869
                     /locus_tag="PL162_08840"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HQF92164.1"
                     /translation="MVALVSMAVCFAGVATLMVALGVAFLHGKDREEALEFVATFLRD
                     SLERRFDALQEGFFSWGSMERALREGDWPFIEEEIHAMARGHGVARVVFSHQGQEIYR
                     AGPLIGDEPPFGGSAGGREGFFLEGRPLIRQRLPVMDDKSRYHGYTALVTVEVSRIFQ
                     PFASFLGHRPRIVPPSPGSVPLGPGLALEVEGGDSHLWALGGMFFFGALGAALMMQPF
                     FRRRDLAAHLMALSSVLAQRDAYTADHSERVGRYALRIARGMKMGRRAQGGLYEAARL
                     HDLGKVLIPDAILLKPGPLTPEEFDVIKEHPAKGADMVFAILERRELAAIIRGHHERW
                     DGSGYPDGLAGEAIPLEARILAVADIIDAMSTARPYHPPRKAEEVRNFLLAEGGKLFD
                     PAVVDAALEALPELQLMGIPRKPLRPRSAEERGNHVLG"
     gene            2912..3289
                     /gene="queD"
                     /locus_tag="PL162_08845"
     CDS             2912..3289
                     /gene="queD"
                     /locus_tag="PL162_08845"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008713224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HQF92165.1"
                     /translation="MFLKREFTFDAAHRLEHYHGKCEALHGHTYRFAVTLRGEPDAEG
                     MVMDFVVLKDLVSERILARLDHSYLNDILPQPTAERIALWIWGELEPALRAFRAELFE
                     VQVWETATCSVILHGEDVRGGRT"
     gene            3286..4506
                     /gene="folP"
                     /locus_tag="PL162_08850"
     CDS             3286..4506
                     /gene="folP"
                     /locus_tag="PL162_08850"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HQF92166.1"
                     /translation="MRPELVHFSDGEELRRALEELGCDPRGFPYFEGKREIYPLRFRG
                     VDTRAANALKQEMLSRGGDVAVHRHAIDRGVATTDCIVFGTAKELRLLAEKLAAMPYW
                     GLDGVRRELVATLEALKRRRWTLPLPRGRSLVLGERTLVMGILNLTEDSFFAGSRVEN
                     DRCLLERARTMAEEGADLLDLGAESTRPGAEARTAEEEIARLVPALRSLREVLPDMPL
                     SVDTTKASVARACVEAGADLVNDISGLGFDPALAPTVAELGVPLVLMHIQGVPRTMQE
                     APHYASLPGDLGAFFEERLARAEGAGLPRERIILDPGLGFGKTPAHNLELLKHLEYFR
                     PWGLPLLVGHSRKSTLGRVLDLPDPGDRLEGTLAVTALCAWQGVEIVRVHDVRENVRV
                     VRTVEAVKHPERWS"
     gene            4768..5145
                     /locus_tag="PL162_08855"
     CDS             4768..5145
                     /locus_tag="PL162_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92167.1"
                     /translation="MRKALVSLLVVSLWLVLGGTALAADFDFMGFAKGTFLNCAHPTT
                     DVAKAKFEFDKEPAQDAADPQITKARVKIFYEGWVKKNSMTVDIFYLETSSLKLVHCE
                     VLEESSGTGEGSCKYTSGWHPAN"
BASE COUNT          862 a         1695 c         1944 g          979 t
ORIGIN      
        1 gggtgtgaac cagggcaaag accggttcga tccccgagga gcaccccgcc agcagagaga
       61 tggtccccgt gggggcgatg gtggttaccg tggcgttgcg gcgcacctcc ccccggcagt
      121 tgggataggc cccccgctcc tgggcgaggc ggcggctttc gtcatgcccc acgctctgga
      181 tctgggccat gactcgcccc cccagggcca gggctccttc ggagtcgtag gggatcccca
      241 tgcgaaagag catttccgcc catcccatga cccccaggcc gaccttgcga tttccctggc
      301 tcatgtcccg gatctccggc aggggatagc tattgacgtc gatgacgttg tccaggaatc
      361 gcaccccgag gcgcaccgcc tcttccagcc gggccccgtc cacggcgcgt tccggagtga
      421 ccatgtgggc caagttcacg gaacccagat tgcaggcctc gtaggggagc aggggctgct
      481 caccgcaggg gttcgtggag acgatggggc ccaggtgggg ggtggggtta tcccggttca
      541 gacggtcgga aaagatcacc ccggggtccc ccgtggccca ggcgcagtgt acgatgcggt
      601 cgaagatctc ccgggccggg agggtgcgca ccggcgcccc cgtgcggggg tttttcagcg
      661 tccattcgcc gtcttgctcc aaggtcctcc aaaagtcgtc cgaaagaccc acggagatgt
      721 tgaaattggc gagcttgccc tcttcggtct tggcccggat gaagtccagc acgtcgggat
      781 gatctccgtc caggatgccc atgttggccc cccgccgggt gcccccctgc ttcaccacgt
      841 ccgtggccag gtcgaacatg cccatgaagg acacgggccc cgaggccacg ccgttggtgc
      901 tgctcaccac atcccccgcc gggcgcaggg aggagaagtt gaaccccgtg ccgccgccgg
      961 atttgtggat gagggcggtg tttttcagag tggcaaagat ctcttccatg ctgtccccca
     1021 cggggaggac gaagcatgcc gcaagctgcc cctggggggt tcccgcgttc atgagggtgg
     1081 gggagttggg gaggaagtcc aggcgcgcca tgaggtcgaa gaagcgatcc tcccaaaggg
     1141 agcgatcccc ctcggcttcg acgctggcga cggcccgggc cacgcgccgg agcagggctt
     1201 cggggggctc gtcgcccttg aggtaacgtt tttttagaat ggtcagagcg ttttcagaaa
     1261 aaatcacaaa aaaacctccc ttctcccggc cgccccgggg cgatggaagg gaaagggccc
     1321 cggcacggtc ccgccgggcc gagcccactg tcccgccggg ggtccatcat actccatccg
     1381 gaggacaaga agaagagggg gtgtttttaa tctatttgat ggcggaactc ctttgcccct
     1441 tcgatttccc gggggtccgt cgtcgaaggc gggtaaaaaa gtgtcttttt accctctgca
     1501 tcgacccttt tcctgtgata aaatgacgtc cgtgtttgga ggcttcttct agaagggagg
     1561 gaggccgttg cgtcggatgg tggccctggt ctccatggcc gtgtgctttg ccggagtggc
     1621 caccctcatg gtggccctgg gggtggcctt tcttcacggg aaggatcggg aagaggccct
     1681 ggagtttgtg gcgacctttc tccgagattc tctcgaacgt aggttcgacg cccttcagga
     1741 aggatttttt tcttggggat ccatggaaag ggccctgcgg gagggggact ggcccttcat
     1801 cgaagaggag attcatgcga tggcccgggg ccacggggtg gcccgggtgg tcttttcgca
     1861 ccaggggcag gagatctacc gcgcggggcc cctgatcggc gatgaacctc ccttcggggg
     1921 aagcgccggc gggcgggagg gctttttcct ggagggaagg cccctgattc gccagcgcct
     1981 tcccgtgatg gatgacaaaa gccgatacca cggctatacg gccctggtca ccgtggaggt
     2041 ctcgcggatc ttccaaccct tcgcctcctt cctggggcat cgccccagga tcgtgcctcc
     2101 ttctccgggg agcgtccccc tggggccggg gcttgccctg gaggtcgaag ggggggactc
     2161 ccacctctgg gccctggggg ggatgttttt cttcggcgcc ctgggggcgg ccctgatgat
     2221 gcagcccttc ttcagacgcc gcgatctggc ggcccatctt atggccctgt cgtccgtgct
     2281 ggcccagcgc gacgcctata cggcggacca ttccgagcgg gtggggcgct atgccctgcg
     2341 catcgcccgg ggcatgaaaa tggggaggcg agcccagggg gggctctacg aggcggcccg
     2401 gctccacgat ctggggaaag tgctcattcc cgacgccatc ctcctaaaac cagggcccct
     2461 gacgcccgag gagttcgacg tcatcaagga gcaccccgcc aagggggcgg acatggtgtt
     2521 cgccatcctg gagcggcggg agctcgccgc catcatccgg ggacaccacg agcgctggga
     2581 tggttcgggg taccccgatg gcctcgcggg ggaggccatt cccctggaag cccggattct
     2641 cgccgtggcc gacatcatcg acgccatgtc caccgctcgc ccctatcatc ccccccgcaa
     2701 ggcggaggaa gtccgcaact ttcttctggc cgaggggggc aagctcttcg accccgccgt
     2761 ggtggatgcg gcgctggaag cgttgccgga actccagctc atggggattc cccggaagcc
     2821 tctccgcccc cgcagcgcgg aggagcgggg gaaccatgtc ctgggatgat cctggggaag
     2881 gtccggaacg ccaagaaagg aggagggggg cgtgttttta aaacgagagt tcacctttga
     2941 cgcggcccac cggctggagc actatcacgg caagtgcgag gccctgcacg gccacaccta
     3001 ccgttttgcc gtcaccttgc ggggcgagcc cgacgccgag ggcatggtga tggacttcgt
     3061 ggtcctcaaa gacctggtgt cggagcggat ccttgcccgc ctcgaccaca gctacctcaa
     3121 cgacattctc ccccagccca cggcggaacg cattgccctc tggatctggg gggagctgga
     3181 gcccgcgctg cgcgccttcc gggccgaact tttcgaggtg caggtgtggg aaaccgccac
     3241 ctgttccgtg atcctccacg gggaagacgt gcgggggggc cggacatgag gcccgagctg
     3301 gtgcactttt ccgacggcga ggagctgcgc cgggcgctgg aggagctggg gtgcgacccc
     3361 aggggcttcc cctattttga gggaaagcgg gagatctatc ccctgcgctt tcggggggtg
     3421 gatacccggg cggccaacgc cctgaagcag gagatgctct cccggggagg ggacgtggcg
     3481 gtacaccgtc acgccatcga ccggggagtg gccaccacgg actgcatcgt cttcggcacg
     3541 gccaaagagc ttcgtcttct ggcggaaaag ctcgccgcca tgccctactg ggggctcgac
     3601 ggcgtgcgcc gggagctcgt ggcgacgctg gaggccctga aaagacggcg gtggaccctt
     3661 cccctccccc ggggacggtc tctcgtgctg ggcgaacgga cgctggtcat ggggatcctg
     3721 aaccttaccg aggactcctt cttcgcgggg agccgggtgg agaacgaccg atgcctgctg
     3781 gagcgggccc ggaccatggc ggaagagggg gcggatctgc tggatctggg ggcggagtcc
     3841 acccggcccg gggcggaggc caggacggcg gaagaagaga tcgcccgcct cgtccccgcc
     3901 ctgcgaagcc tccgggaggt cctgccggat atgccccttt cggtggacac caccaaggcc
     3961 tcggtggccc gggcctgcgt ggaggcgggg gccgatctgg tgaacgacat ctccgggctg
     4021 gggttcgatc cggccctggc ccccacggtg gccgagctgg gggtgcccct ggtgctcatg
     4081 cacatccagg gagttcctcg caccatgcag gaggcccccc actacgcctc cctccccggc
     4141 gatctggggg cctttttcga ggagcgcctg gcccgggctg aaggggcggg attgccccgg
     4201 gagcggatca tcctcgaccc cgggctgggg tttggcaaga ccccggccca caacctggag
     4261 ctcttgaagc acttggagta ctttcgcccc tgggggcttc cccttttggt ggggcactcg
     4321 cgcaagagca ccctggggcg ggtgctggac ctccccgacc ccggcgaccg gctggagggg
     4381 accctggcgg tgaccgccct ctgcgcctgg cagggggtgg agatcgtgcg ggtccacgac
     4441 gtgcgagaaa acgtccgggt ggtgcgcacc gtggaggcgg tgaaacatcc ggagcgctgg
     4501 agctagggac ctcggggggg gggtgtgggg cgccttgtcg caggggcggg cgagaaaaaa
     4561 cctcccgggg acgcgacgtt ttcccttcgc ggtggtacaa taaaggtgct cggagaagcg
     4621 actgcgctct gtggcgggag gtgggagagg ggccggggtt tggatgaaag ggcacccccg
     4681 gtggcgtggc ccgaaggagg agaggggaaa gccccctctc ggcggtgcgg tttggtctcg
     4741 atcatctttt tggaggagtg atgtgtcgtg agaaaggctc tggtttcttt gttggtggtt
     4801 tccctgtggt tggtcctggg gggcacggcg ctggcggctg attttgactt catgggcttc
     4861 gccaagggga cgttcctgaa ctgcgcccac cccaccacgg atgtggcgaa ggccaagttc
     4921 gagttcgaca aagagcccgc ccaggacgcg gccgaccccc agatcaccaa ggcccgggtg
     4981 aagatcttct acgagggatg ggtgaagaag aactccatga ccgtggacat cttctacctg
     5041 gagacctcgt cgctgaagct cgtccactgc gaggtcctgg aggaatcctc gggcaccggc
     5101 gagggaagct gcaagtacac cagcgggtgg cacccggcca actaggcggc ggggcgtcgc
     5161 gggggtagtt ttttgtcttt cgaggggagg ggggaccctc ccctgtttcg ttcgggccac
     5221 ccgaaaagag ccctgaggag gccttcgagc ccccccgagg ccggggagga ccctttggcc
     5281 cggagggcgt cccgccgggg tgcggggaag ggggaagggt aatgcgtttc gttcctcggg
     5341 gggttctgaa gcgcctgagg tccttggtgg cggtgatcgc ggcggcggcg ctcctcagcg
     5401 gggtgctcct cggggtgctg gcgctcctgc gccccctgtt gcccccgggg gcccaggact
     5461 ttcttttgca cctttccgac
//
LOCUS       DAOOER010000094         5373 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_14675_length_5373_cov_9.569951, whole genome shotgun sequence.
ACCESSION   DAOOER010000094 DAOOER010000000
VERSION     DAOOER010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14675_length_5373_cov_9.569951"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..649)
                     /locus_tag="PL162_08860"
     CDS             complement(221..649)
                     /locus_tag="PL162_08860"
                     /inference="COORDINATES: protein motif:HMM:NF025124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQF92168.1"
                     /translation="MNAPGLPEIPEAVFEELLVNALVHRDYLVSAPIRLFVFDNRIEI
                     ISPGHLPNNLTVEKIRAGNSNIRNPILVSYVAKGLLPYHGLGSGIKRALGKWPTIDFT
                     DDHDGGLFTATVHRRPTKVQKNSFPMKASIVRRKIKMLFC"
     gene            832..1050
                     /locus_tag="PL162_08865"
     CDS             832..1050
                     /locus_tag="PL162_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92169.1"
                     /translation="MPTILKDPGTRRAVYRIIRDLPPEDVEKVASYAAFLAQQRRLED
                     EEDLHAVEERRSQDSLPLRKILQEMGLA"
     gene            1189..1302
                     /locus_tag="PL162_08870"
                     /pseudo
     CDS             1189..1302
                     /locus_tag="PL162_08870"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1829..2011)
                     /locus_tag="PL162_08875"
     CDS             complement(1829..2011)
                     /locus_tag="PL162_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92170.1"
                     /translation="MARRDEFSGDLWRIPAERMKKRRPHLVPLSIQALAVLDELRPLI
                     GNDPLLFPSLRDRKNP"
     gene            complement(2065..>2591)
                     /locus_tag="PL162_08880"
     CDS             complement(2065..>2591)
                     /locus_tag="PL162_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92171.1"
                     /translation="VHGASFKKTLGQYPEVGLKDARLRRDKLRASMRRGDDGLEEVTF
                     ASLAEEWFSRKVESVRAPGHSRTVRSRLDRLILPYLGHRPPGAISAPEVLAVLRRIED
                     RGHVETAHRAAQIVGQIFRYGIAVGKADRDVAADLRGAIIPRTPNPLPHHHRPRENRR
                     PHAGDSRLRAAPQW"
     assembly_gap    2592..2616
                     /estimated_length=25
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2862..3210)
                     /gene="ssrA"
                     /locus_tag="PL162_08885"
     tmRNA           complement(2862..3210)
                     /gene="ssrA"
                     /locus_tag="PL162_08885"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(3401..3868)
                     /gene="smpB"
                     /locus_tag="PL162_08890"
     CDS             complement(3401..3868)
                     /gene="smpB"
                     /locus_tag="PL162_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HQF92172.1"
                     /translation="MERKNVAQNRRARFEYFILETFEAGIVLTGTEIKSVREGRVNLK
                     DGYAKVEGGEMWLLNVHISPYEKGTHYNHDPVRPRKLLMHRREIRKLLAAVREKGLTL
                     VPLAIYIKDSRYAKVELALAKGKNVHDKRESVQDRDAAREISRAARRGGREDD"
     gene            complement(4170..4247)
                     /locus_tag="PL162_08895"
     tRNA            complement(4170..4247)
                     /locus_tag="PL162_08895"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4210..4212),aa:Pro,seq:cgg)
     gene            complement(4282..5295)
                     /locus_tag="PL162_08900"
     CDS             complement(4282..5295)
                     /locus_tag="PL162_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92173.1"
                     /translation="MVLEVTFIPAGGPDVGGGHVLRCRSLAAALELRGVSCRWFAPAE
                     ARSLFSPGDDVGAWGDFFGPEGPDALSRSVPPGSVVVVDSYLPSPAWFEEAGRRWRLV
                     VVDDLRDIAVEEYAVAVLNPNPGAEDLPYRRRSGVGLLLGVSYALLRPAFWDLASEGG
                     HDVFAVAGASDPQGVLIQLVRWWRPCWPFLRAVAGPLMPAEEVATLRREVRERANVEV
                     LQAPEDFPERMARAGRVLCTSSGTSLEALALRKPLAVFQVAENQRRIGEEIARRKWGI
                     NLGFWGTWKAENLAPFLEMPPQASPGAVNPQGARRAAEDLEGLLKGAPGQRAASGSGG
                     EDR"
BASE COUNT         1078 a         1802 c         1512 g          956 t
ORIGIN      
        1 ggaaggtcgc agagagggca taaagaaagg cgtcgagaaa ggtcgtaaag agggcctccg
       61 cacctcggcg cggaacatgc tccgaaaggg catgccgatg gcgctggtgg ccgaagtgac
      121 gggactttcc ccggacgagg tggcggcgct gatggattga agcctagaca gacatcaata
      181 ccgatcgacg ggaaaatagt cctaacccgc atttcgcacc tcagcaaaag agcatcttga
      241 ttttcctgcg aacaatggat gccttcatgg gaaagctatt tttttgcacc tttgtcggcc
      301 tccgatgaac cgtggcggtg aacagaccgc cgtcgtgatc gtcggtaaag tcgatggtcg
      361 gccattttcc cagcgcccgc ttgatgccag accccaaacc atgataagga agcaacccct
      421 tggccacgta ggaaaccaaa atcgggttac gtatgttgga gttgccagcc cgaatctttt
      481 ctaccgtcag gttgttgggg agatggccgg gactgatgat ttcgattcgg ttgtcgaaga
      541 caaacaggcg gatcggggcg ctcaccagat aatcacggtg caccagagca ttgaccagca
      601 gctcctcgaa aaccgcctcg gggatttccg gcaatcccgg cgcattcacg ccgcgtccgg
      661 cctgtacctt tgacggcctc atcgccctcg cggactactt cgacgtgagc ctcgattacc
      721 tcgtgggacg gtcggacgat ccggggcgct gaagggacga gctattgaca ccctccccga
      781 gccctgtaca atttggttgt ttcatgatgc cccgcgaaag gagtgtttct cgtgcccaca
      841 atcctcaaag atcccggaac ccgtcgcgcc gtgtaccgca tcatccgcga ccttcctccc
      901 gaggacgtcg aaaaggtggc cagctatgcc gccttcctgg cgcagcagcg gcgccttgag
      961 gacgaagagg acctccacgc cgtcgaagag cgccgatccc aggattccct gcctctccgg
     1021 aaaattctcc aggagatggg tcttgcctga ccgctacgac gttcagataa aatccggcgc
     1081 cgccagggaa ctcaaaagtc ttccccgcga cgtggcggaa ctcatcgccc gggtactcct
     1141 ttctcttgag gagaaccccg tcctcggggg tgcctcaagc tcaccggaag cgacgcctac
     1201 cgtctccgtc ttggcgacta ccgcatcgtc tacgagatct tcgacaagcc ccttgttgtc
     1261 ctcgtcatcc gcatcgccca ccgtcgcgac gcctaccgct gatcccgcac gcagttttca
     1321 aggttcaccc cgccaacctt ccgatcagcc ccgccagatc cgtcccttcc ggcgtgtccg
     1381 cgcggccgtc cgccagatcg accatggccc tcaccaggct gcactccccc aatgacctga
     1441 tcccgatgac ggcttcgatg tcgatcgggc cgccttcttg cgcccccggc gctaccccgc
     1501 cgggggcggg gtagggtcca ggaggtcgct ggcttgacac ccacaaatgt gcgctatacg
     1561 cctaatcatc tccattcccg gaaactgcct gccagattcc caacgacaca ccgtggaaag
     1621 ggatacgccc aaagcttctg cgaactggct gtattttgca acaaacaaga attgccctcc
     1681 acgtgggcaa gctgaagttc tatggcgtcg gggggccaaa gtctcgactc gttggcattt
     1741 gtcgagaaca tggagcggaa ggagtgcgcc gtgaattcgt cctggggata tcccagccgc
     1801 cggagggccg cgttcaccgt ggcgtcgctc atgggttttt tcggtcgcgc agcgagggga
     1861 agagcaaggg gtcgtttccg atcaaggggc gaagttcgtc cagaaccgcc agggcctgga
     1921 tggagagggg gaccagatgg gggcggcgct tcttcatgcg ttcggcgggg atgcgccaga
     1981 ggtctccgct gaattcgtcc ctgcgggcca tgcgcacttc tccaggcctc gcggcggtga
     2041 aagcttgcaa caggagggca cacctcacca ctgcggtgcc gctcgaaggc gtgaatcgcc
     2101 cgcatgaggc cgcctatttt ctcggggtct gtgatggtgg ggtagtgggt ttggggtgcg
     2161 gggaatgatg gccccccgaa gatctgcggc cacatcccta tcggccttcc ccacggcgat
     2221 gccgtaccgg aagatctgtc ccacgatctg ggccgcccgg tgagccgtct ccacgtgccc
     2281 ccggtcctcg atccgccgaa gcaccgccaa cacttccggc gcggagatgg ccccaggggg
     2341 gcgatgcccc aggtagggaa ggatgaggcg atccagccga gacctgacgg ttcttgaatg
     2401 tcccggagca cgcacgctct cgaccttccg agaaaaccat tcctcagcca gcgaggcgaa
     2461 ggtgacttcc tccagcccgt catccccccg gcgcatggat gcccgcaact tgtcgcgtcg
     2521 gagcctggca tccttcaacc ccacctccgg atattggccc agggtcttct tgaaggaggc
     2581 cccgtggaca annnnnnnnn nnnnnnnnnn nnnnnnccga atcctgaagg gtgcatcttg
     2641 cggtctgcgg tcctggcctc cgcagctctg acttccctct ccgtgagcaa ttggattccc
     2701 cctgccactc gagcattcgc ccccgaggag ccgggggcaa actggtggca aatatgatac
     2761 ccgatagggg caatttttgc gaccatggtg ggggagtcca ttcttaacaa aagagccgcc
     2821 gtcagggttt gcgatcatcc caacggcggc attttaagta ttggtggagg cgcggggaat
     2881 cgaaccccgg tccgacaggg gccagccgtg gaggcggcta caggcttagt ctccgctttg
     2941 gggtcgacga aaaactcccg gagacgggat ttcttcgctc tagctcccta gatcttagcc
     3001 gaagaaaaag gagcccttcc ctcggagcaa ccccagtttg tgacgccctt tcgcagccct
     3061 gggatccgac cgcgaaggac gtgactgctc gttaggcagc cagtgcgtag ttatcgttgg
     3121 cgattattgt tttgccgcag tttatcgagc cacggcacct cgacctgctc ctccagaccc
     3181 tccaacctgc cgtcgaaacc gtacgccccc ttctgaggga taaaaccgtt gtgaacgatc
     3241 accacaaaaa cacgccatca aacagggtgt cgcgcaaaaa aacgctcttc acgaacaggg
     3301 acttgaggaa acatccacaa cagagagccc ccggacgcga aatacctctc caacgggcga
     3361 aattgcccgc ccgaaaccgc catccttagc gaacataacc tcagtcgtcc tcgcggcccc
     3421 cccggcgcgc cgcccgagag atctcccgcg ccgcatcccg atcctgcacg ctctcccgct
     3481 tgtcatgcac attcttgccc ttggccagag ccagctccac ctttgcgtaa cgcgaatcct
     3541 tgatgtagat cgccaagggc accaatgtca gccccttttc ccgcaccgcc gccaggagct
     3601 tgcgaatctc ccgacggtgc ataaggagct tgcgagggcg caccggatcg tggttgtagt
     3661 gcgtgccctt ctcgtagggg gaaatgtgca cgttcaaaag ccacatctcg cccccttcca
     3721 ccttggcgta gccatccttg aggtttacac gcccctcccg gacggatttg atctccgtgc
     3781 ccgtgaggac gatacccgcc tcgaaggtct ccaaaatgaa gtattcaaac ctcgcccggc
     3841 ggttctgggc cacgtttttg cgctccatcg catcgccctc cgccgacatt ctagcccctt
     3901 tctggataaa catcaagatt agtcaggacc gcaaagtccg ccccccacag gccgccgaag
     3961 gcgatacgac ggccacaccc aaaaggcggc cgcagggttg ccattcccca agatcacccg
     4021 ggcgccccgg ccaccgaccg accgcgcccc cgccatggcg gccccgaagg cctctccact
     4081 ccggccggaa agcgatcctc ccgccgaaag acactgaggg gagctccgtt tcccggaccc
     4141 cccttggcga gcgaaaaacc cctgggaact ggtcggggtg agaggatttg aacctccgac
     4201 ctcttggtcc cgaaccaagc gcgctaacca gactgcgcta caccccgaac ggccgcattt
     4261 tagcaccctt tttcggaagc gtcaacgatc ctccccgcca gagcccgatg ccgccctctg
     4321 gccgggggcg cccttcaaaa gtccctccag atcctcggcg gccctccggg ccccctgggg
     4381 gttcaccgcc cccggagaag cctgaggagg catttctaga aaaggagcga gattctccgc
     4441 tttccaggtg ccccaaaaac ccaggttgat tccccatttc cgccgggcaa tctcttcgcc
     4501 gatgcgccgc tgattttccg ccacctggaa gaccgccaaa ggttttcgaa gagccagagc
     4561 ctccagcgaa gtgccgctgg aagtgcaaag cacccttccc gcccgggcca tgcgctccgg
     4621 aaagtcttcg ggggcctgga ggacctccac gttggcacgc tcccggacct cccgccgcag
     4681 ggtcgccact tcttccgcag gcatgagggg gcccgccacc gcccggagaa aaggccagca
     4741 gggacgccac cagcgcacca gttggatcag aactccctgg ggatcggagg cccccgccac
     4801 ggcaaaaacg tcatgccccc cttcggaggc aaggtcccag aaggcggggc gcaagagggc
     4861 gtaggaaacg ccgagaagaa gccccacccc cgaacgccgt cggtagggaa ggtcttcggc
     4921 ccccggattg gggttcagca ccgccacggc gtattcctcc acggcgatat cccgaaggtc
     4981 gtccaccacc accaggcgcc aacgcctccc cgcctcttca aaccaggccg gagaaggcag
     5041 atagctatcc accaccacca cactccccgg cggaacgctc cgggaaaggg cgtccggccc
     5101 ctcgggaccg aaaaagtccc cccacgcccc cacgtcatct ccgggagaaa agagcgaccg
     5161 cgcttcagcc ggggcaaacc accgacatga taccccccga agctccaagg ccgccgccaa
     5221 cgaacggcag cgcaggacgt gccctccgcc aacatccgga cccccggcgg gtataaaggt
     5281 cacttccagg accatccctc cttaggttgt gttttcaaaa gctgaaagag tcgcctgcgg
     5341 gcctcggata cggcgatacg gcccatggca tca
//
LOCUS       DAOOER010000095         5359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_14725_length_5359_cov_6.308069, whole genome shotgun sequence.
ACCESSION   DAOOER010000095 DAOOER010000000
VERSION     DAOOER010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14725_length_5359_cov_6.308069"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PL162_08905"
     CDS             complement(<1..265)
                     /locus_tag="PL162_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92174.1"
                     /translation="MAVGKGDPVGDGPVVGEGSAVGEGSVVGAGVSVGVGVTLGAGVP
                     EGAGVALGAGVPEGAGVALGVAVGVGVIRARASMVSFTRMRGLV"
     gene            306..959
                     /locus_tag="PL162_08910"
     CDS             306..959
                     /locus_tag="PL162_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92175.1"
                     /translation="MIEGVKQGETPLDPEDAPQTGGATGTNVAIPPEVLRAILGNVDA
                     EHFQVVSLGDALETVFSTGSGMVELRVVSEPTLQGGVIYRWTLLAWNNKTSRWEVAAS
                     GEIVTASGRRAAAFEATATTLTTRLQDGGSFDLDGVVNGVVRARTATTLLGAPGMAPT
                     VAPTGSPAPSPSVVPPTATPSVPSVGSSGGGCNSGLPGFLLPTLLLGTPLLLLRKRP"
     gene            1276..3183
                     /locus_tag="PL162_08915"
     CDS             1276..3183
                     /locus_tag="PL162_08915"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HQF92176.1"
                     /translation="MRIVQHLGVARDDQQLEELWALGTRLIPELEARAREEKALREGQ
                     GLLVELTPEDFERRVTEEAQVNVRNLRNVEHVLEGPFEVWGEVFSRLGFEGIFGTGVR
                     DIGLTNLLKLCLTAKLFDGGSKLRAREWINETLGLELGKDRIYRMMDRLATRIPQVKE
                     RGFSCGRHLVEGPVSLLLFDVTTLYFESFEEDGDHALSDPVDGGNGDRVGGTEGEGPG
                     AAESLFREDRENTRDFPQDPDLPCRGLRKKGYSKDNKINETQVVLALSTSEEGIPLWY
                     DLFPGNMSEGKTLLESMAGIGKKLDPRETWLVADNAMLNTANRTALEKNGYTYVLGSS
                     LRKLPASEKEAALSREEHATLRSPLPWTSCEKDSVGAGASEEWSWRTLRRSNGNTLVV
                     TWNAAKAKRDAHKREHLRTRLEKRLGSRGEIRGKELISNRGTLKYVEPVGEDSWYRLR
                     QEALEEDARYDGFHAVETNFPVATKEDALRVLAAYGQLWHIEDCFRVAKSELKIRPVF
                     HWTKPRIEAHVALCFLALLMERFLEKQIKSKRHMTLSPQQIRHALGDIQSALVRDTQS
                     GTLYRMPLGLKGSGREIYRALGLKRQVEPTEITSLAKYRHHIPKCALVVGTCRPIPDD
                     QTFSQAEERGN"
     gene            3499..3939
                     /locus_tag="PL162_08920"
     CDS             3499..3939
                     /locus_tag="PL162_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SYNERG-CTERM sorting domain-containing protein"
                     /protein_id="HQF92177.1"
                     /translation="MPNFLIELSLCKDLGDFCAKVGKTDKTATRGSRTTLWYQGNPNY
                     VGDGTLTGSSTPSTAFYDGTASGISLTGFSAEGSTMTCTVAGGVPQPTPTTVPTATPA
                     PTVAPTATPSPGGSSGSSGGGCVGGATPWTLVLFLPLLGTVRRK"
     gene            4039..4509
                     /locus_tag="PL162_08925"
     CDS             4039..4509
                     /locus_tag="PL162_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92178.1"
                     /translation="MVWRGIQGGVWRRGVLVACVLFLGVGAGEANLSGTSGDVASADA
                     RRALAERAREMKTVRIIVQLRVPFVPEGELKSTWQVFRQREGIAQAQDRVLERLRGLG
                     CDGVRTAKRFETVPQMGLKVDEACLEALGQDPDVQSIQEDALGAPGGASLAAPQ"
     gene            4591..>5359
                     /locus_tag="PL162_08930"
     CDS             4591..>5359
                     /locus_tag="PL162_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQF92179.1"
                     /translation="MEKRRVQQLVAGVLAAALVWGVGGAEAACRLNAPAALRAKAAAA
                     GSVRVIVELKTDFRPEGSLGKAQAFMQRNGITALRGQILARLGAGVASASVKAFETIP
                     YFAAQVDQNGLEALLNDAGVASVQEDKLSRPHLAQSVPLIHAPEAWAAGYSGSGWAVA
                     VLDTGVAKDHPFLAGKVVSEACYSTTNAGEPCQSVCPGGASESTAAGAAVPPVGGGYG
                     EDFYHGTHVAGIVAGHDASSSGVARDGNIIAVQVFSYF"
BASE COUNT         1018 a         1676 c         1769 g          896 t
ORIGIN      
        1 tcaccaagcc ccgcattcgg gtgaaggaaa ccatcgatgc cctggccctt attaccccca
       61 cgcccacggc gacgccgagc gccacgccag ctccctcggg aactccggcg ccaagcgcca
      121 cgccagcccc ctcgggaact ccggcaccga gcgttacgcc gactcccacg gacacccccg
      181 cccccacaac ggagccttct cccacagcgg agccttctcc cacgacaggg ccatccccca
      241 cagggtctcc tttgcccacg gccattcccc agcccaccca accccctacc gagccccaac
      301 ctctggtgat cgagggagtc aaacaggggg aaacgcccct ggatcctgag gatgctcctc
      361 aaaccggcgg tgccacggga accaacgtcg ccatcccccc cgaggtgctc cgggccatcc
      421 tgggaaatgt ggatgccgag catttccagg tggtgtcgct gggagacgcc ctggaaacgg
      481 ttttctccac cggcagcggc atggtggagc tccgcgtcgt ttcggaaccc accctccaag
      541 ggggagtgat ctaccgttgg accctcttgg cctggaacaa caagacctcc cggtgggaag
      601 tggcggcatc gggggagatc gtcacggctt cggggcgacg ggcggcggct tttgaggcca
      661 cggccacgac cctcaccacc cgcctgcagg acgggggctc gttcgatctg gacggcgtgg
      721 tgaatggcgt ggtgcgggcc cgcaccgcca ccaccctgct gggagctccg ggcatggccc
      781 ccactgtagc ccccacagga tccccggccc ccagtccttc tgtagtaccc cccactgcca
      841 cgccttccgt cccttcggtg ggttcttcgg ggggcggatg caacagcggc cttccgggct
      901 tcctgctccc caccctcctg ctgggaacac cgttactgct gctacggaaa cgtccgtaag
      961 tccgctccca aggggctcct ccggcgatgc cggaggagcc ccttttttcc cgactttcgc
     1021 ataggcgaat gcccgccggg taaaggtcag cattggtcac gaaacgcccc aaggatttgc
     1081 aagggtttcc tgtgcccggg ctctcgctcc ttctcggaga gatcgaaaat agtgcctagc
     1141 aaatattact tgacaaacgt ttctctttct gctatcatct tcccatgtat atccggagta
     1201 agacctccaa gctcagtccc cgtaaaacgg tccagatcgt cgaggcctac gtcacgccgg
     1261 aaggcaaacg accaagtgcg catcgtccag catcttggcg tggcccgcga tgaccagcag
     1321 ctcgaagagc tgtgggccct cggcacccgg ctcattcccg agttggaagc ccgggcccgg
     1381 gaagaaaagg ccctgcggga aggtcagggg cttctcgtgg agttgactcc ggaggacttc
     1441 gagcgccgcg tcaccgaaga ggcccaggtg aatgtgagaa atttgcgcaa tgtggagcat
     1501 gtgctggaag gccccttcga ggtctgggga gaggtctttt cccggctggg gttcgaaggg
     1561 atcttcggca cgggggtgcg cgacatcggt ctgacgaacc tgctgaagct ctgtctgacg
     1621 gcgaagctct tcgatggggg gagtaagctc cgagcccggg agtggatcaa cgagaccctg
     1681 gggctggaac tgggcaagga ccggatctac cggatgatgg accggctggc gacgcggatt
     1741 ccccaggtga aagagcgggg attttcctgc ggacgccatc tggtggaggg gcccgtttcc
     1801 ttgctgctct tcgatgtcac caccctctac ttcgagagtt ttgaggaaga tggggaccac
     1861 gctctttccg atccggtcga cggggggaac ggggaccggg tcggggggac cgaaggagag
     1921 ggaccggggg cagcggagag ccttttccgg gaagaccggg agaacaccag ggattttccc
     1981 caagatccgg accttccgtg ccggggattg cgcaaaaaag gctacagcaa ggacaacaag
     2041 atcaacgaaa cccaggtggt gctcgccctg agcacctcgg aggaaggcat tcccctctgg
     2101 tacgacctct ttcccggcaa catgtccgaa gggaaaaccc ttctcgaaag catggcgggg
     2161 atagggaaga aactcgaccc ccgggagacg tggctcgtgg cggacaacgc catgctgaac
     2221 accgccaacc gcaccgccct ggagaagaac ggttatacct atgtgctcgg ctccagcctc
     2281 cgcaagcttc cggcttcgga gaaggaagcc gctctgagcc gagaggagca cgccactctc
     2341 cgctcccctc tcccctggac ctcatgcgaa aaggattccg ttggcgccgg agcatccgaa
     2401 gagtggtcct ggcgcaccct tcgccgatcg aacggcaaca ccctcgtggt gacctggaac
     2461 gccgccaagg ccaaacgcga cgcccacaag cgggaacatc tccgtacccg gctcgaaaag
     2521 cgcctgggtt cccggggaga gatccggggg aaagagctca tctcgaaccg gggcaccttg
     2581 aagtacgtcg aacccgtggg agaagacagc tggtatcgcc tgcgccagga agcgcttgaa
     2641 gaagatgcgc gctacgacgg cttccacgcc gtggaaacca atttccccgt cgccaccaag
     2701 gaagacgccc tacgggttct tgccgcctac ggccaactgt ggcacatcga agactgtttt
     2761 cgggtagcga aaagcgaact gaagattcgc cccgtattcc actggacgaa accccgcatc
     2821 gaggcccacg tggccctctg ttttctggcc cttttgatgg agcgcttcct ggaaaagcag
     2881 atcaagagca agcgccacat gacgctctct ccccagcaaa ttcgtcacgc cctgggggac
     2941 atccaatccg cgctggtccg ggacacccaa agcggtaccc tgtatcggat gccgctgggt
     3001 ctgaagggct ccggacggga gatctaccgc gccctcggcc tgaagcgcca ggtggagccc
     3061 acggaaatca cgtcgttggc gaagtaccgc caccacattc cgaagtgcgc tctggtggtg
     3121 gggacctgcc gtcccatccc cgacgatcag acgttttcgc aagcggagga gcggggcaac
     3181 tgaacaagga actgtcccct cttcagcgaa gggaactctc cgggcgccag agaagaagga
     3241 cccggagcct gggccggact tggccgggaa attcctctca gagctgaaaa aggccgtacg
     3301 tgtaggggcg gttctctccc ccgcccctac acggcccgca ggaaacattt gctcggaaaa
     3361 gtccggccca agcgccacgc tccgcccgcg gagggcctgg agagttccca gcgaagcagc
     3421 gttcgaagca ccacaccatc cggaacacaa caggcactgc gaagccacca ttcttctctg
     3481 gggaaagggg tcttcgtagt gcctaacttc ttgatagagt tgagtctttg caaggatcta
     3541 ggagattttt gtgcgaaagt cgggaagacg gacaaaactg ccacccgggg atcgcgtacc
     3601 accctctggt accagggaaa tccgaactac gtgggagatg gcacgctcac ggggagcagc
     3661 accccgagta cggcgttcta cgacggaacc gcttcgggga tctccctgac gggcttctcg
     3721 gcggagggat ccaccatgac ctgtacggtg gcggggggag tcccccagcc gacccccacc
     3781 accgtgccca cagcgacacc ggcgcccacc gtggccccca cggcgacccc ctctccgggg
     3841 ggaagttctg gaagcagcgg aggagggtgt gtcggaggcg cgacgccctg gaccctggtg
     3901 ctcttcctgc cccttctggg gacggtgaga aggaaataga tcgatccgga aagcacgtca
     3961 ggccgtggtg cgacgcttgc gcggcgggga aaaatcccta caatggaccc tgatgttggt
     4021 cgaaaggggg cgaggcgtgt ggtgtggcgt ggcattcagg gaggtgtgtg gcgtcgaggg
     4081 gtcttggtgg cgtgtgtgtt gttcctgggg gtgggagcgg gagaggcgaa cctctcgggc
     4141 acttcggggg acgtcgcctc ggcggatgcc cggagggcgc tggcggaacg ggcccgggag
     4201 atgaagaccg tgcggatcat cgtgcagctg agggttccct ttgtccccga gggagagctc
     4261 aagagcacct ggcaggtctt tcggcagcgg gagggcattg cccaggccca ggaccgggtg
     4321 ctggaacgcc tgagggggct gggatgcgac ggcgtgcgga cggcgaaacg gttcgaaacg
     4381 gtgccccaga tggggctcaa ggtcgatgaa gcgtgtttgg aagccctcgg gcaggatccc
     4441 gacgtgcaga gcatccagga ggacgcgttg ggggctcccg gaggagcttc tctggccgct
     4501 ccgcagtaac cggcggggac gggagaagag ctcggaacgg cgcggcatat ttctgacagt
     4561 ggcgttgcag attagtgaag gggtgatgtc atggagaagc gcagagtgca acagttggtg
     4621 gcgggcgttt tggcggcggc gttggtctgg ggagtgggag gagcggaggc ggcgtgtcgg
     4681 ctgaacgccc ctgcggcgct ccgagccaag gcggcggcag cggggtccgt gcgggtcatc
     4741 gtggagctca agacggactt ccgccccgag ggaagcctgg ggaaggccca ggcgttcatg
     4801 cagcgcaacg gcatcaccgc cctgcggggc cagatcctcg cccggctggg ggccggagtg
     4861 gcgagcgctt cggtgaaggc cttcgaaacc attccctact tcgcggccca ggtggaccag
     4921 aacggtctgg aagcgctgct gaacgatgcc ggcgtggcct cggtgcagga agataagctt
     4981 tcccggcccc acctggccca gagtgtgccc ctgatccacg cccccgaagc ctgggcggcg
     5041 ggatattcgg ggagtggatg ggccgtggcg gtgctcgaca ccggtgtggc gaaggaccac
     5101 cccttcctcg cgggaaaggt ggtctccgag gcgtgctact cgaccacgaa cgccggtgag
     5161 ccctgtcagt ccgtctgccc cgggggcgct tccgagtcca ccgccgctgg cgccgccgtg
     5221 ccccccgtgg gagggggtta cggagaggat ttctaccacg gcacccacgt ggcggggatc
     5281 gtggcggggc atgacgcctc ttcttccggc gtggcccggg acggaaacat catcgcggtg
     5341 caggtctttt cctactttc
//
LOCUS       DAOOER010000096         5101 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_15629_length_5101_cov_5.395561, whole genome shotgun sequence.
ACCESSION   DAOOER010000096 DAOOER010000000
VERSION     DAOOER010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15629_length_5101_cov_5.395561"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            301..609
                     /locus_tag="PL162_08935"
     CDS             301..609
                     /locus_tag="PL162_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92180.1"
                     /translation="MTIVKEQAPSRESLQNMSRSFAESAQSAAANTELLSGVDQAANA
                     MESIREAGDQNVEGMKDMESAAQGLKDLGQHIADLIGRYTLKSGVTDGRTPRGGGEAP
                     "
     gene            complement(615..3782)
                     /gene="carB"
                     /locus_tag="PL162_08940"
     CDS             complement(615..3782)
                     /gene="carB"
                     /locus_tag="PL162_08940"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF003671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HQF92181.1"
                     /translation="MHDKNNTVLVLGSGPLRIGQAAEFDYAGSQACRALREEGCAVVL
                     LNSNPATIQTDDTVADVVLLRPLLPEVVEEILQTYRPRGVVATLGGQTALNLCVACEE
                     AGLWKRHGCEVLGTSPKAIARAESREAFRQTMVALGQPVPPSVCVASVGEAERFAATV
                     PLPLIVRPDFTLGGSGNGVVRHRKELALRVEEGLRASPVERVLVERYLEGWREIEAEV
                     VRDGAGNALCVCGMENVDPMGVHTGDSVVVAPVLTLTDRQWQRLRGAALAIAGALEVR
                     GACNVQFALSPDGEEYAVIEVNPRASRSSALASKATGYPIARVAARIALGRLLTELPN
                     PVTGAGSALAEPALDYVVLKIPAWPFDAFPGADEALGTRMKATGEILALGASFAQALL
                     KGYRSLEGGDRWHDPRWHGMTEGALWHHVLAPTHRRLESMGELLRRGVAPEELGRRTG
                     IHPYFVAQLLEVVEGERRVRQGGASAKTLGEGRRLGFGVERLAALAGLPVETVEHLLA
                     FAGYAPAFREVDGCAGESPAGSGYFYSVPGGVDELPGEHAGGVAVLGSGAIRIAQGME
                     FDYCCVKAVEALRRRGVRGIMVNCNPETVSTDADISDALYVEPLTPGDVLPVLRRERV
                     RGVLACFGGQTSLRLGQALDERGVLILGPSPRVVFRTEDRGEFASLLKNLGIPQPPGR
                     GVRSLGEAWEVVEELGFPLMVRPSFVLGGVAMGVAHGKEEFGLLVSGALAAVPDQTVL
                     LDKFLAGQEFEVDALGDGEDVFLPGVFEHLDPAGIHSGDSIAHFPDISLSSAQRQEIQ
                     RMVTALSGALELRGLLNVQFVLHQGRLHVIEANPRASRTVPMGAKVAGVPLVDRAVGM
                     ALGERLRDSGPLGWGERPGKFGVKVPVFSTEKLPGIDPRLGPRMLSTGEALGLGTSLG
                     QALAEGFRGAGWRLPPAGRVFFSVRDGEKEAVGPVGASFAALGWHLSASPGTAEVLRR
                     HGVPVETVARESVEARKAQEWDLVVNVAGTGGLHEGMGWRRRAVEAGVPYLGSLEAAW
                     GVWRALAFLRGDPIQEPPGANRNRT"
     gene            complement(3775..4827)
                     /gene="carA"
                     /locus_tag="PL162_08945"
     CDS             complement(3775..4827)
                     /gene="carA"
                     /locus_tag="PL162_08945"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HQF92182.1"
                     /translation="MQELWLTLEDGSSWKGWGEPPVEGVEGEVVFTTAAGGYPQALSD
                     PSYCGQMVLFAFPPAGIYGVDLDALESSRPWATAALVGSLERAPGRNVTQLAPWLAAS
                     GVPLVAGMDTRGLVRHLRSRGTLRGRLAKEVGSPALPVSQDAVRRVSPARVTVEGSGS
                     RRVGVLDCGVKEGILRHLRQEGWTVVRFPSATPPEEILSWGGAGLILSNGPGDPLLWS
                     RETATVRHLLGRIPIFGICLGCQILARACGGNTEKLPFGHRGVNQPVVDLSTGRGFVT
                     SQNHGYAVNEASLTDTELAVTFRHLGDGSVEGLRHRRVAAEGVQFHPEASPGPRDALF
                     LLDRFLASLSGENGHA"
BASE COUNT          963 a         1888 c         1467 g          783 t
ORIGIN      
        1 gaccggggac ctgctgatta caagtcagct gctctaccaa ctgagctatg ccggcccaca
       61 aaaaatgcta tagaaccatc ttttttctcg tgccctggga gccaaacacc aaaagggact
      121 cccaaaacgc cttgggtggc tggtcgctgc agtgacgtct ctcgggtgga aggggagctt
      181 tctaaaaacg cagtttcccg ggctttctcc cggaaccacg ttttccctat tttttgcgcc
      241 gtatcgcccc cgaagagcaa aaaaagatct tctccacaga catttattct atcacggggc
      301 atgaccattg taaaggaaca agccccttcc cgggagtcgt tgcagaacat gagccggagc
      361 ttcgccgaat ccgcccagtc cgccgccgcc aacaccgagc tcctttcggg ggtggaccag
      421 gcggccaatg ccatggagag catccgcgag gcgggagatc agaacgtgga gggcatgaag
      481 gacatggaaa gcgccgccca aggcttgaag gacctggggc agcacatcgc ggatctcatc
      541 ggccgctaca cgctcaaaag cggtgtgacg gacggaagaa cgcctcgggg gggcggagaa
      601 gccccgtaag gacatcacgt ccggtttctg ttcgctcctg ggggctcttg gatcgggtct
      661 ccgcgcagga aggccaaggc ccgccacacg ccccatgcgg cctccaggct ccccaggtag
      721 ggcacccccg cctccacggc ccgccgacgc caccccatgc cttcgtggag ccccccggtg
      781 cccgcgacgt tgactaccag atcccactcc tgggcctttc gggcctccac cgactcccgg
      841 gcaacggttt ctacgggaac accatggcgt ctgaggactt cggccgtgcc cggcgacgcg
      901 ctcaggtgcc atcccaaggc ggcgaaggac gcccccaccg ggcccaccgc ttccttttct
      961 ccgtcccgca cgctgaagaa gacccgcccc gccgggggca atcgccaccc cgccccccgg
     1021 aacccctccg ccagggcctg tcccagcgat gtgcccaacc ccagggcctc gccggtggaa
     1081 agcatccggg gacccaaccg ggggtcgatc ccaggcagtt tttccgtgga aaagaccggc
     1141 actttgacgc caaattttcc ggggcgctct ccccacccca gggggccgga atcccggaga
     1201 cgctccccca acgccatccc caccgcccga tccaccagcg gcacccccgc caccttggcc
     1261 cccatgggca cggtgcggct cgcccgggga ttggcttcga tgacgtgcag gcgcccttgg
     1321 tggaggacaa actgcacgtt gagaaggcca cgaagctcca gcgcccccga aagggccgtc
     1381 accatgcgct gaatttcctg acgctgggcc gacgaaaggg agatgtcggg gaaatgggcg
     1441 atggagtccc cggaatgaat tcccgcggga tccaggtgtt cgaaaactcc tggaaggaac
     1501 acgtcctccc cgtcccccag ggcgtccacc tcgaactcct gaccggcaag gaatttgtcc
     1561 agcaacaccg tctgatccgg caccgccgcc agggccccgg acaccagaag tccaaactcc
     1621 tcttttccgt gggccacccc catggccacc cccccaagca cgaaactcgg gcgcaccatg
     1681 aggggaaagc cgagttcctc caccacctcc cacgcttcgc caagactccg cacgccccgc
     1741 cccggaggct ggggaatgcc caggttcttg aggagggagg cgaactctcc ccggtcttcc
     1801 gttcggaaaa ccacccgggg cgaaggaccg aggatgagca ccccccgttc atccagggcc
     1861 tgccccaggc gcagggaggt ctgcccgccg aagcacgcca gcacgccccg cacgcgctcc
     1921 cgtcggagca cggggagcac atcccccggc gtcaagggtt ccacgtacaa cgcgtcggaa
     1981 atgtccgcat cggtgctcac cgtttcggga ttgcagttca ccatgatgcc ccgcacaccc
     2041 cggcgccgca gggcttccac cgccttgacg caacagtagt caaactccat gccctgggca
     2101 atgcggatgg cccccgaccc caacaccgcc acgcctccgg cgtgttctcc ggggagttcg
     2161 tccactcccc cggggacgct gtagaagtat cccgatcccg cggggctctc ccccgcacag
     2221 ccgtccacct cacggaacgc gggggcgtac cccgcgaacg ccaggaggtg ctccaccgtc
     2281 tcaacaggaa gccccgccag agcagcgagg cgttccacgc cgaaccccag acgccgcccc
     2341 tctcccaacg ttttcgccga cgcccctcct tggcgcaccc gacgctcccc ctccaccacc
     2401 tccagaagct gggccacgaa gtaggggtga atgcccgtgc gacgccccaa ctcttccgga
     2461 gcgacacccc gtctcagcag ttctcccatg ctctccagcc gccgatgggt gggcgccagc
     2521 acgtggtgcc acaacgcccc ctcggtcatg ccgtgccacc gggggtcatg ccaccgatct
     2581 cctccttcca gagaacggta tcccttcaga agggcctggg caaacgacgc ccccagggcc
     2641 agaatttccc ccgtggcctt catgcgggtg cccagcgcct cgtccgcccc cggaaaggcg
     2701 tcgaagggcc aggcgggaat tttgagcacc acatagtcca gggcgggttc cgccagggcg
     2761 cttcccgccc cggtgacggg gttgggaagc tccgtaagaa gccgccccag ggcaatgcgg
     2821 gccgccaccc gggcgatggg atatcccgtg gccttgctgg ccagggcgct ggagcggctg
     2881 gccctgggat tcacctcaat gacggcgtac tcctcgccgt cggggctcag ggcaaactgg
     2941 acgttgcagg ctccccgcac ttccaaagct cccgcgatgg ccaacgccgc cccccggagc
     3001 ctttgccact ggcggtccgt gagggtgagc acaggggcca ccaccacgga atctcccgtg
     3061 tgcaccccca tggggtccac attttccatg ccgcagacgc aaagggcatt gcccgccccg
     3121 tcccgcacca cctcggcctc aatctcccgc cacccctcaa ggtagcgttc caccagcacc
     3181 cgttccaccg gagaggcccg caggccctcc tccacccgga gggcaagctc cttccggtgt
     3241 cgcaccacgc cgttccccga tcctcccagg gtgaaatccg gtcgcacgat gagcggcagg
     3301 ggcaccgtgg cggcgaaacg ttccgcttcc cccacggagg ccacgcagac gctgggaggc
     3361 accggttgcc ccagggccac catggtctgg cgaaaggcct cccggctttc cgcccgggca
     3421 atggccttcg gagaagtccc caaaacctcg cagccgtggc gcttccaaag acccgcctcc
     3481 tcgcaggcca cacagaggtt cagcgccgtt tgccctccca gggtggccac cacgccccgg
     3541 gggcgatagg tctggagaat ctcttccacc acctcgggga ggaggggacg cagcagcacc
     3601 acgtccgcca cggtgtcatc ggtttggatg gtggcggggt tgctgttcag aagcaccacc
     3661 gcacacccct cttcccgcag ggcccggcag gcctgactgc cggcgtagtc gaactcggcg
     3721 gcctggccga tgcggagcgg gccggagccc agcacgagca cggtgttgtt tttgtcatgc
     3781 atggccgttt tctccggaaa gggacgcaag aaaacgatcc agaagaaaga gggcatcccg
     3841 gggccccgga gatgcttcgg ggtgaaactg caccccttcg gcggccactc gccgatgacg
     3901 aaggccctcg acggagccat cccccaagtg acggaaagtc accgcgagct ccgtgtccgt
     3961 caaagaggcc tcgttcaccg catacccatg gttctgactg gtgacaaagc cccgccccgt
     4021 ggaaagatcc accaccggtt ggttgacgcc ccgatgtccg aaggggagtt tttccgtgtt
     4081 cccgccacat gcccgcgcta agatctgaca ccccagacag atgccgaaaa tggggattcg
     4141 ccccaggaga tgacgcaccg tggcggtttc ccgactccac agcaacggat cccccggccc
     4201 gttgctgaga atcaatcccg ctccccccca ggagaggatc tcctccgggg gagtggcgga
     4261 ggggaaccgc accacggtcc acccctcctg ccgcagatgg cgcagaatgc cttccttgac
     4321 cccacagtcc aacacgccga cccgacgaga tccgctcccc tccaccgtga cgcgggcggg
     4381 ggaaacccgc ctcacggcgt cctgggaaac cggaagcgcc ggagatccca cttcttttgc
     4441 cagacgcccc cggagcgttc cccggctccg aaggtggcgc accagtcccc gggtgtccat
     4501 ccccgccacc agcggcaccc ccgatgcggc gagccatggg gccaactggg tcacattcct
     4561 tccgggggcc ctttccaaag agcccaccaa cgcggcggtg gcccaggggc gggagctttc
     4621 cagagcgtcc agatccacgc cgtagatccc cgcaggagga aaggcgaaga gcaccatctg
     4681 cccgcagtag gagggatcac tcagggcctg gggatatccc cccgccgccg tggtgaacac
     4741 cacctccccc tccacccctt ccaccggggg ctctccccat cccttccacg agctaccgtc
     4801 ttccaacgtg agccaaagtt cttgcacgag acacctccat agacatgaaa aaagccagga
     4861 ccttccggcc ctggctcctc gacgctacaa aagacacacc gacgtcaagg gacccttcgg
     4921 aagaggccgc tttccacctt ttccccaccc cacacatgtc ggcatcgtcg cttcataaaa
     4981 aatcacccct ggtttaaaac ttttggccat tataaccctg gttctttttc cgtcaagccc
     5041 ccaaacgatg ttgtcgcccg agaatgcgcc caggggggtg ttccgtccca tggacaccgg
     5101 a
//
LOCUS       DAOOER010000097         4948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_16234_length_4948_cov_8.282649, whole genome shotgun sequence.
ACCESSION   DAOOER010000097 DAOOER010000000
VERSION     DAOOER010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16234_length_4948_cov_8.282649"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="PL162_08950"
     CDS             <1..812
                     /locus_tag="PL162_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQF92183.1"
                     /translation="KLAGEPGKAQVSEFQLPAGQGSPVIMSNFTSIQEQEAPVQITRT
                     DKQRVSKLMADLGPELPLGTAVDLLSRRIDGAAIMPPGYDYRFRGDYEIMSEAGAAFA
                     EAGIMAVLLTYLALSAILESFTWSLLILTTVPLGLVGVLWALYLTGTSISIFVLLGVV
                     MLVGIVVNNAILVVDRMRLLRDEGVDPVEAMYQALDDSFRAVVMVTLAAVLGMLPLAV
                     ASGLGSELNNGIGIASAGGIAMSSLLALFVVPLIFLVGKSFRRKKEELPQA"
     gene            1082..3706
                     /locus_tag="PL162_08955"
     CDS             1082..3706
                     /locus_tag="PL162_08955"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HQF92184.1"
                     /translation="MKKSGAFAALAMIWAWALFGAVPGEGTEKFWLVTRHIAPVMRSP
                     GTYTTVEDGRGIEDQEGIEACVYYGSRVTVAPVSGKHGAQWVKLMFHGSSLGYVEKEA
                     LVPFPSYEPRGPEPYVVRPERLSLFLLPGSLPAEGYSSFYLPRGVTVTGIGTTAVDGQ
                     GWVLLRFSSVGASPEYPAPGDVQTRYAWAREDDLIRLASYEPSLSRVDPEEVPGEIML
                     DQGVDDIHRVPLSPGDREKLASQGFFIQKAPVILKNIELDDLVEVYPRTRDHVTPLFI
                     TVDLGLHTFHLIFDRMLQQAEERVFVPTLDLFLKALQEGLEGHRKSLEASVLGRSVRE
                     DVGDFLAVARKLLTEEGSLSPRGEEEYRRIMEAQGRLDSPFSGKEEDYTFFRPRGHYT
                     LSPGLERYFRAMAFLGGVTFPLKGRDQERLLRSTGVIALFCTLLEDPAVGKAWKKLFD
                     PFTLLVGASNDNSWYDYAPVVKKLLGGALPLEDEGRVEELRQALMKASRPPLILGSPA
                     PRLGASQEEQEDQALGFRLLGRRFTFDALVFQALTSPRTGTNDHPRNLPDPLDALAVL
                     GSPVALRETKAFEVFRGYAENREALRGRWKTFHQDPLGDTIYSDVLRLYATYFEPTEG
                     GQFFAASPAWEYRKLVSAAASWAELKHDTILYGEQSGAEMGDGGDVWYAPDFLCPDPR
                     GYVEPEPRLFRGLAAAAGRIRQFLDQAELPDGEYAEKFSTFAALMERLAHLAELEVTG
                     APLGDEEYRIIREFPAELTRELLLPADQLGLYPPLLPEVQDRLRMALVADVASDFAAG
                     RALEVGVGAPRYMYVFVNDRWGGPRVTRGAVFSFYAFDRSLAEGRLTDEEWKKMVYQG
                     DPAALDRLRPDWTRVPEQ"
     gene            3703..4536
                     /gene="amrB"
                     /locus_tag="PL162_08960"
     CDS             3703..4536
                     /gene="amrB"
                     /locus_tag="PL162_08960"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HQF92185.1"
                     /translation="MRSIALVAGVLVGVFLFPSGAQALGCPPLPPRVHRALEGPRDPR
                     DSGVEGRVLGGLTPHHDLAGELMVRFYEELRRGAPHPRRILLIAPDHHRAGRGAVTFC
                     GAPWETDRGRVSADEEGVAMLGEHGVARRDDALFPREHGITVHLPLLAAFFPGVPVLP
                     LVVRSSVSDLELLALRKSLMPLVGAGGLVILSMDLSHGKSSAEALRQDRRTLPVLAQL
                     RWKELRGLDLDSPRGGALFLALLEELGARRGVVRDHATAASFGEPPEAPGTSYATVIF
                     F"
BASE COUNT          783 a         1517 c         1684 g          964 t
ORIGIN      
        1 taaagctcgc cggggaaccg ggcaaggccc aggtgtcgga gttccagctc cccgccggcc
       61 agggatcccc cgtgatcatg agcaacttca cctccatcca ggaacaagag gctccggtgc
      121 agattacccg aacggacaaa cagcgggtga gtaagctcat ggcagacctg gggccggaac
      181 tgcccctggg tacggcggtg gatctcctct cccggcggat cgacggggcg gccatcatgc
      241 ctccgggtta cgactaccgc ttccgggggg actacgagat catgtccgag gcgggggcgg
      301 ccttcgccga ggcggggatc atggcggtgc ttttgaccta cctggccctg tcggccatcc
      361 tggagtcctt tacctggtcc ctcctgatcc tcaccacggt gcccctgggg ctcgtggggg
      421 tgctctgggc cctctatctc acggggacga gcatttccat cttcgtgctg ctcggggtgg
      481 tgatgctggt gggcatcgtg gtcaataacg ccatcctcgt ggtggaccgc atgaggctcc
      541 tgcgggacga gggcgtggac cccgtggagg ccatgtacca ggctctggac gactccttcc
      601 gggcggtggt catggtgacc ttggcggcgg tactgggcat gcttcccctg gcggtggcct
      661 cggggttggg gagcgagctc aacaacggca tcggcatcgc ttcggcgggg ggcatcgcca
      721 tgtcctccct gctggccctc ttcgtggtgc cgctgatatt tctcgtgggg aaaagctttc
      781 gcagaaaaaa agaagaactc cctcaggcct aaaacggcct tttgaggtat aatggttcca
      841 cgaaagagcc tccatgcgca tcctccgacc tcgggttttg gacgggtcgg gggacttgga
      901 cgggcctcac agcggcggca gacctccctc tccgcccctg tggggctttt tattttgcct
      961 ccttgccccg gagcccgata tccggattct tggggccttt gcctcggcgt tctttttgcc
     1021 ctatgatggc cccgcgtccc ccagggatgc actcatcttt tttcgaaagg cgtgatttcc
     1081 ggtgaaaaaa agcggcgcct tcgcggcctt ggcaatgata tgggcctggg cgctcttcgg
     1141 agcggttcca ggggaaggga cggagaagtt ctggctggtg acccgccaca ttgcccccgt
     1201 gatgaggtct ccggggacct atacaacggt ggaagacggc cgaggcattg aagaccagga
     1261 gggcatcgag gcctgcgtct actacggcag tcgggtgacg gtggctccag tttccggcaa
     1321 gcacggggcc cagtgggtga aattgatgtt tcatggatct tccctggggt acgtggagaa
     1381 agaagccctg gtgccctttc cctcctacga gccccgaggg cccgagccct acgtggtccg
     1441 tccggaaagg ctatccctct tcctcctccc gggatccctt ccggcggagg gctacagctc
     1501 tttttacctc ccccgaggcg tgacggtcac cgggatcggc accaccgccg tcgacggcca
     1561 aggttgggtg ctcctgagat tttccagcgt gggggcttcg ccggagtatc cggcgccggg
     1621 ggacgtccag acccggtatg cctgggcccg ggaagacgat ctcatccggt tggcctccta
     1681 cgaaccctcc ctctcccggg tggatcccga agaggtcccc ggggagatca tgctggacca
     1741 gggagtcgac gacattcatc gcgtcccctt gagccccggg gacagggaaa aactggcgag
     1801 tcagggtttt ttcatccaaa aagccccggt gatcctgaaa aacatcgagc tggatgacct
     1861 ggtggaggtc tatccccgca cccgggacca cgtgacgccc ctttttatca ccgtggatct
     1921 ggggctgcac accttccatc tgatcttcga ccgcatgctc cagcaggccg aagaaagggt
     1981 tttcgtcccg acgttggatc tcttccttaa ggccctccag gaggggttgg aggggcatcg
     2041 gaaatccctg gaagcgagtg tcctggggcg gtcggtgcgg gaggacgtgg gggacttttt
     2101 ggcggtggca cgaaagcttc tcaccgagga aggatccctt tctccccggg gagaagagga
     2161 gtatcgccgc atcatggaag cccagggaag gctcgactcc cccttctcgg gaaaggaaga
     2221 agactacacc ttttttcgcc cccgggggca ctacaccctc tctccaggac tggagcgata
     2281 cttccgggcc atggccttcc tgggaggcgt caccttcccc ttgaaaggca gggaccagga
     2341 gcggctcctg cggagcacgg gggtcatcgc cctcttctgc acgctcctgg aagatcccgc
     2401 cgttggcaaa gcgtggaaaa agctcttcga tcccttcacg ctgttggtgg gggcctctaa
     2461 cgacaattcc tggtacgact acgctccggt ggtgaaaaaa cttctcggag gggccctccc
     2521 cttggaggac gaggggcggg tggaagagct ccgccaggcc ttgatgaagg cgagccgccc
     2581 cccgctgatc ctggggagcc cggccccccg gttgggggct tcccaggagg agcaagagga
     2641 tcaggccctg ggatttcgcc ttttggggcg gcgcttcacc tttgatgccc tggtgttcca
     2701 agccctgacg tcgcctcgta cgggcaccaa cgaccacccc cgaaacttgc ccgatccctt
     2761 ggacgccctg gcggtgctgg gatctccggt ggcccttcgg gagacgaagg ctttcgaggt
     2821 cttccggggg tacgccgaaa accgggaggc cctccggggg cggtggaaaa ccttccacca
     2881 ggatcccctg ggggacacga tctattccga tgtgctacgg ctctacgcca cgtacttcga
     2941 gcccaccgag ggggggcagt ttttcgccgc ctccccggcg tgggagtatc gcaaattggt
     3001 gtccgccgcc gcctcctggg cggagcttaa gcacgacacg atcctctacg gcgagcagtc
     3061 cggggcggag atgggagacg gaggagatgt ctggtacgcc ccggacttcc tctgtcccga
     3121 cccccggggc tacgtggagc ccgagccccg acttttccgg gggttggccg ccgccgcagg
     3181 gcggatacga cagttcctgg atcaggcgga gctgcccgat ggggagtacg ccgaaaagtt
     3241 ctccaccttc gccgccctca tggagcgcct ggcccacttg gcggagctgg aagtgacggg
     3301 ggctcccctg ggagatgagg agtaccgcat catccgggag ttcccggcgg agctgacccg
     3361 ggagctgctc ctcccggcgg accagctggg cctctaccct cccctgcttc ccgaggtgca
     3421 ggatcgcctt cgcatggcgt tggtggcgga cgtggcctcg gatttcgcgg cgggaagggc
     3481 gctggaggtg ggcgtcgggg ccccccggta tatgtatgtt ttcgttaacg atcgctgggg
     3541 gggaccccgg gtgacccggg gggcggtgtt ttccttctat gccttcgacc gttccctggc
     3601 ggaggggcgc ctcaccgacg aggagtggaa aaagatggtc taccagggag accccgccgc
     3661 tctggaccgc ctgcgccccg attggacccg ggtgccggag cagtgagatc catcgccttg
     3721 gtggcggggg tcctggtggg ggtctttttg ttcccttccg gggcccaggc cctggggtgt
     3781 cctccgcttc cccccagggt ccatcgggcc ctcgagggcc ccagggatcc ccgagattcc
     3841 ggagtggagg gaagggtcct gggggggctc actccccacc atgacctggc gggggagctc
     3901 atggtacggt tttacgaaga actgcggcgg ggggcgcccc accctcgccg aatcctcctc
     3961 atcgcccccg accaccaccg ggcggggcgg ggagccgtga cgttttgtgg ggccccctgg
     4021 gagaccgatc gaggaagggt gtccgccgac gaagaagggg tggcgatgct gggagagcac
     4081 ggcgtcgcga ggcgagacga cgcccttttc ccgcgagagc acggcatcac ggtgcacctg
     4141 ccccttttgg cggcgttttt ccccggcgtg ccggtgcttc ccctggtggt gcggagctcc
     4201 gtctccgatc tggagctcct ggcactgcgg aaaagcctca tgcccctcgt gggggccgga
     4261 ggcctggtga tcctcagcat ggacctttcc cacggaaaaa gttccgccga agcgctgcgc
     4321 caggatcgcc gcaccttgcc ggtcctggcg cagctccggt ggaaggagct ccggggattg
     4381 gatctggact ctccccgagg gggggcgctc tttctggcgc tcctggagga gctgggggcc
     4441 cggcgaggag tggtgcgaga tcacgccacg gcggcctcct ttggggagcc tcccgaggcg
     4501 ccggggacga gctacgccac ggtgatcttt ttctgacccc ggctcggggc gttcccctct
     4561 ggacgacctc aggcaacgcc ttggggtggg ttttccccga cggtcgcttt cttttgccgg
     4621 gaggtgcgca tcttccagcc ggctttgccg cagaatgggg gctacgatgg tgagctttct
     4681 cccggaggag gagaacatct cgtggtgcct aactctttgt agaaagctag tgatggaaag
     4741 gactcagggt ttttttgtgc gaaagtcggc aacgccttgg ggtggatttt cctccgaggg
     4801 cagatcgcaa gggtctccgt tctctgacga gaagggggcc cttgtggtga tcggcggcgg
     4861 tacttcgggg acaaaggggg tcaggagtcc cggggagggt cttcgaaggc ccgggagaat
     4921 tggggtatca gaaagggcgt ggcggccc
//
LOCUS       DAOOER010000098         4884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_16480_length_4884_cov_5.385794, whole genome shotgun sequence.
ACCESSION   DAOOER010000098 DAOOER010000000
VERSION     DAOOER010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16480_length_4884_cov_5.385794"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            200..1735
                     /locus_tag="PL162_08965"
     CDS             200..1735
                     /locus_tag="PL162_08965"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HQF92186.1"
                     /translation="MRSVSMLNDVLGPIMRGPSSSHTAAAWRISTLARGLLGERVKEA
                     EFTFDPWGSWGVVYDQQGSDLAFAAGLLGWSLEDGRFFDALKQAPGEGLAISFRLEPL
                     PWATHPNDVRLRLRGQEGDAMEIRGRSIGGGAIEVTAVDEVPVLLRGDSEELFVETSP
                     SDFSSVAQCLASQGAVESHRMIHREGMLLRCSLSSSPSADLIASLRALPEARRLRTAS
                     PFLHVIRGKPLFESAEEACAQGEKSLGALGRLSESHLLGLDEGAVEEEMRRREGVMTQ
                     AVLQGLRDENVAMRLLPPSAGGVLRAEAEGRLPLGGVTTRAAARALAAMHVNSSMGVV
                     CAAPTGGSAGVLPGVVTTLRQDCGRSEEEILRGLFAAGAVGVIIATRATFAAEEAGCQ
                     VEIGAAGAMAAALVVEAFGGGAERACAAAAVALQNTMGSPCDPVRGVVEIPCHTRNAV
                     AASCAFICADLVTGGYINPVPLDETIDAAYAAGKMLPPELRCTARGGLALCPSARGMV
                     LRR"
     gene            complement(1749..>3374)
                     /locus_tag="PL162_08970"
     CDS             complement(1749..>3374)
                     /locus_tag="PL162_08970"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQF92187.1"
                     /translation="SAVPRVVGLSGALMATLMVLIVLLLQDLFSSEQHLHKAKVALEE
                     EIRHKEEAQKTLQGTLSRLDLATQAGGIGVWSCGRESHFLSWNDQMYDLFGLPIGSPL
                     SCDGWLHMINSEDRAFLEAFLEGAARSGEAFQMEVRVMTPEGIRRHLGLAARMARSEP
                     QGEGTFIGVCWDLTALKETELSLRRQGSAMKFLAEVSSRCIDLPLEELPGAIQSALGR
                     MGVLVGARRAYAMGLGDYPGAAPFVYEWCAGDEPCDLRGFPLEDLPSWVAVHRREDRA
                     FAVEVASLPPGEMRRHLEGRGCGGLLVVPMFYGEEPRGVVGFEGLLPWKNLSDQDGAL
                     FGLFAQIVVNARRRIQAEERIRHLATHDFLTDLPKLPLARDRLTVALGAARRYRHRVA
                     VMFIDLDGFKAVNDTFGHDAGDEVLRHVARSLRSGLREMDTAARVGGDEFLLVIPELQ
                     VPDHAIIVAEKVLRALKAPIPVPGGTARVGASIGIALYPDHGSNDESLIKAADDAMYR
                     EKFAKKGGYQFATPLSSSGDGEKGSERPPRGER"
     assembly_gap    3375..3474
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3475..4252)
                     /locus_tag="PL162_08975"
     CDS             complement(<3475..4252)
                     /locus_tag="PL162_08975"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HQF92188.1"
                     /translation="MGLGLYVLVALLWGVVESRSRQVLRDRIRDEGIFYASHLQSELD
                     FRMVSLRRMVHRWQMRGGTPRDEFMADAESYVKDFVGLRALAWVDKGFIIRWAIPREW
                     EGEVANRYLAFDSHRRLTLEKARDRRAMAMTPPLDLVWGGGRGFLMAFPLEARGAFDG
                     FILVVVRYDDWLGKLFEVNDNHMVPGHILARVLVDGVPVYRQEGWEDLGDPAWGAEEP
                     LDFEGYRFVVQVRPTPSFFEAHRSAVPRVVGLSGALMATLM"
     gene            4562..>4884
                     /locus_tag="PL162_08980"
     CDS             4562..>4884
                     /locus_tag="PL162_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HQF92189.1"
                     /translation="MAAAQKANDDQIPMTREGYDLLKKELISLRTEGRSEISRKLEEA
                     RAFGDLSENAEFHAAQEEQAQLETRVLWLETRLSKAKIVDMAEVDASKVALGTTVTIL
                     DLDTQA"
BASE COUNT          891 a         1606 c         1521 g          766 t
ORIGIN      
        1 acggggttcg cccaccgccc gagcaccggg tgaacccatt gtaacacgaa gccccaggaa
       61 ggacaatcgg gagcctctcc ggctgtggct ctcctggggc caaagaagca aggtcccggg
      121 tctggagctt tagtggtatg cttaccccgg gcgccaaagg ccctaggaag gcgccaatct
      181 ttcccttagg agggatttca tgcgaagcgt gagcatgttg aacgacgtgc tggggcccat
      241 catgcggggg ccatcgagct ctcacaccgc cgccgcctgg cgcatctcca ccctggcccg
      301 gggccttctg ggggaaaggg tgaaggaggc cgaatttacc ttcgacccct ggggttcgtg
      361 gggagtggtc tacgatcagc agggcagcga tctggccttt gcggcggggc ttttaggatg
      421 gtccctggag gatgggcgct ttttcgacgc cctgaagcag gctcctgggg agggccttgc
      481 catctccttc cgactggagc ccttaccctg ggccacccac cccaacgacg tgcgccttcg
      541 ccttcgcggg caggaggggg acgccatgga aatccggggg cgatccatcg gcggaggagc
      601 catcgaagtg acggcggtgg acgaagttcc ggtcctgctc cggggagact cggaggaact
      661 cttcgtggag acttcccctt cggacttctc ttccgtcgcc caatgcctgg cctcccaggg
      721 ggccgtggaa agccaccgga tgatccatcg ggagggcatg ctcctgcggt gctccctgtc
      781 atcctcccct tccgccgacc tcatcgcctc cctccgggcc cttccggagg cgcgccgcct
      841 ccgcaccgct tcgcctttcc tgcacgttat ccgggggaag cccctctttg aaagcgccga
      901 agaagcctgc gcccaggggg aaaaaagcct gggggccctg gggcggctct cggaatccca
      961 tctcctgggc ctggacgaag gcgcggtgga agaagagatg aggcgccggg agggcgtcat
     1021 gacccaggcg gtgctccagg gcctgcggga cgaaaacgtg gccatgcgcc tcctgcctcc
     1081 cagcgccgga ggcgtcctcc gggcggaggc ggaagggagg ctccccctag gaggggtgac
     1141 cacccgggcg gcggcccggg ccctggcggc catgcacgtg aacagctcca tgggagtggt
     1201 atgcgccgcc cccacggggg gatcggcggg ggtgctgccg ggggtggtga cgaccctgcg
     1261 ccaggactgt ggccgtagcg aagaagagat cctccggggg cttttcgccg ccggggcggt
     1321 gggggtgatc atcgccaccc gggccacctt cgccgccgag gaggcgggat gtcaggtgga
     1381 aatcggcgcg gcgggggcca tggcggcggc cctggtggtg gaggccttcg gagggggggc
     1441 ggaaagggcc tgcgcggcgg cggcggtggc tctccagaat accatggggt ccccctgtga
     1501 ccccgtgcga ggcgtggtgg aaatcccctg ccacacgcgc aatgccgtgg ccgcctcctg
     1561 cgccttcatc tgcgccgacc tggtcacggg ggggtacatc aaccccgtcc ccctggacga
     1621 gaccatcgat gcggcctacg ctgcaggaaa gatgctgccg ccggagctgc ggtgcaccgc
     1681 ccggggagga ctggccctct gcccctccgc ccggggcatg gtgcttcgcc ggtgagggcc
     1741 ctcggagcct atctctcgcc ccggggtggc ctttcggatc ccttctctcc gtcgccagaa
     1801 gacgagaggg gggtggcgaa ttggtaccct cctttttttg caaacttctc ccggtacatg
     1861 gcgtcatccg ccgccttgat gaggctttcg tcgttcgacc cgtgatcagg atagagggcg
     1921 atgccgatgc tggcccccac ccgggccgtc cctccgggca ccgggatggg ggccttcagg
     1981 gcccgtagga ccttctcggc gacgatgatg gcgtgatcgg gaacttgaag ctccgggatc
     2041 accagcagga actcgtctcc ccccacccgg gccgccgtgt ccatttcccg aaggccgctc
     2101 ctcaggctcc gggccacgtg gcgcaatacc tcgtctccgg cgtcgtggcc aaaggtatcg
     2161 tttaccgcct tgaagccgtc gaggtcgatg aacatcaccg ccacccggtg gcgatagcgg
     2221 cgggccgctc ccaacgccac ggttagacga tcccgggcca agggaagttt gggcaagtcc
     2281 gtcaggaaat cgtgggtggc caggtgacga atgcgttctt cggcctgaat cctccgccgg
     2341 gcattgacga cgatctgggc aaaaaggcca aagagggccc catcctggtc ggacaggttc
     2401 ttccagggga ggagaccttc gaagcccacc accccccggg gttcctcgcc gtaaaacatg
     2461 gggacgacca aaagaccacc gcagccccgc ccctccaggt ggcgtcgcat ttcccccggg
     2521 ggaagggacg ccacctccac ggcaaaagcc cgatcctccc ggcgatggac ggccacccag
     2581 gacggaaggt cctccaaggg aaagccccgg aggtcacagg gctcgtcccc ggcgcaccat
     2641 tcgtagacga aaggggcggc cccggggtaa tcgccgagcc ccatggcgta ggctcgacgg
     2701 gcccccacaa ggacccccat acgccccagg gcgctctgga tggccccggg gagctcctcc
     2761 agggggaggt cgatgcaccg agaagagact tccgcgagga acttcatcgc cgatccctgg
     2821 cgcctcaggg atagctccgt ctccttcaac gccgtcagat cccagcacac cccaatgaaa
     2881 gtcccctccc cctggggctc cgatcgcgcc atccgggccg caaggcccag atggcgccgg
     2941 atcccctcgg gggtcatgac gcgaacttcc atctgaaacg cctccccgga gcgggcggcg
     3001 ccttccagga acgcttctaa aaaggcccgg tcctcagaat taatcatgtg gagccagcca
     3061 tcgcaggaga ggggagagcc gatgggaagg ccgaagaggt catacatctg gtcgttccac
     3121 gagaggaaat ggctttcccg gccgcagctc cacacgccga tgccccccgc ctgggtggct
     3181 aaatccaggc gggagagggt cccttgtagg gtcttctggg cctcttcctt atgccggatc
     3241 tcttcctcca gcgcgacctt ggccttatga aggtgttgct cggaagaaaa gagatcttga
     3301 agaaggagca cgatgagcac catgagagtg gccatgaggg ccccggatag ccccaccacc
     3361 cgggggactg cggannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccatga
     3481 gagtggccat gagggccccg gatagcccca ccacccgggg gactgcggag cgatgggcct
     3541 cgaagaaaga gggcgtgggg cgcacctgta cgacgaagcg atacccctca aaatccaagg
     3601 gctcctccgc tccccaggcc gggtcgccga gatcttccca gccctcctgg cggtagaccg
     3661 gcacgccatc caccaagacc ctggcaagga tgtggccggg caccatgtga ttgtcgttca
     3721 cctcaaagag cttccccagc caatcgtcgt agcgcaccac caccaggatg aatccatcaa
     3781 aggccccccg ggcctccagg gggaaggcca tcaggaaccc ccttccccct ccccagacca
     3841 ggtccagagg gggggtcatc gccatggccc ggcgatcccg ggccttctcc agggtgaggc
     3901 ggcgatggga gtcgaaggcc aagtagcggt tcgccacctc cccctcccat tcccggggaa
     3961 tggcccaccg gatgatgaag cccttgtcca cccaagccaa ggccctaagc ccgacaaaat
     4021 cctttacgta gctttcggca tcggccataa attcatcccg gggggtaccg ccccgcatct
     4081 gccaccgatg gaccatgcgg cgcagggaaa ccattcgaaa gtctaactcc gactgcaggt
     4141 gggaggcgta aaaaatccct tcgtcccgga tacgatcccg gagcacctgc ctgctacgcg
     4201 actccacgac tccccagagc agcgccacga gaacatagag ccccagtccc accagcaagg
     4261 gagctcctct tttccccaag aaagatccgc gaaaaaaacc tcccccaccg gacccggggt
     4321 ccgatgagaa atcttcccgc gacgccattt atctcccacc cacgccccgg ggctccgggg
     4381 caaattttcg ctgtctgcca tcataccaca gctccgacgc cccaccgaag ctccgctcct
     4441 cgggcgaact ttttccggag aaaaacctgc cacaaaacac ctaacttatt gccccttccg
     4501 ggccggtctg atacaataca ccatcggcat ttccgtttct tcttgaggag gcgttccgta
     4561 gatggcagct gcacaaaagg caaacgacga ccagatcccc atgacccgcg aggggtacga
     4621 cctgctgaaa aaggagctca tctccctgcg caccgagggt cgttcggaga tctcccgcaa
     4681 gctcgaggag gctcgggcct tcggagacct gagcgaaaac gcggaattcc acgccgccca
     4741 agaggagcag gcccaactgg aaacccgcgt cctctggttg gaaaccaggc tgagcaaggc
     4801 aaaaatcgtg gacatggccg aggtggacgc gagcaaggtg gccctgggca ccacggtgac
     4861 catcttggat ctggataccc aggc
//
LOCUS       DAOOER010000099         4835 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_16704_length_4835_cov_7.478033, whole genome shotgun sequence.
ACCESSION   DAOOER010000099 DAOOER010000000
VERSION     DAOOER010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16704_length_4835_cov_7.478033"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PL162_08985"
     CDS             complement(<1..818)
                     /locus_tag="PL162_08985"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HQF92190.1"
                     /translation="MPVVKAPPQWITLPECWSILRREVGPVLRRDEAWCSPEEALDRP
                     LTRDVVAQRNVPHFRASAVDGFALRSEALGGASPASPVFVGGNDAVWCNTGAPVPSPY
                     DAVVMVEDVTEDPASGGIRVHRALPPGMNVRAVGEDAMARRLIARQGDRVTPALAALC
                     VAAGVGTLPVRPRMRVTYIPTGDEIIAPDRWLATASPSEGLVGETNSLLLKGYLNRWG
                     VQLAVAPVVADDPALLAQALRDACAGADVVVLGAGTAKGDRDHAASVLSSQGRIL"
     gene            complement(806..2035)
                     /locus_tag="PL162_08990"
     CDS             complement(806..2035)
                     /locus_tag="PL162_08990"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HQF92191.1"
                     /translation="MAGFVEHLTARREALRRVAEALSFPWDLPSEKKPLLEVQGGAAA
                     RFVRAPEPVPAFDRSLRDGYAVRSVDVRGASPSSPAFLSVKGEVPMGSLPSFSGTSGS
                     AAALYTGGGVPEGYDAVIMAEDTALMGNLLEVRRAAAPGDFIVKAGEDVSAQSVVLDR
                     GEGVSPGVSLALAALGITALEVSCLRVGILSTGDEIVPAETFPLPLGCVRDANETFLT
                     LLFRRMGCHVTAYGIVPDVPATLQEVFRRAESENDVVLLSGGSSVSTRDLCLDLLRTI
                     SDPGLLVRGLNLSPGKPTLIGGSRTPFRLVVGLPGHPLSCAVVALTVVVPLVHMLRGD
                     GEGEDSPILVRTGDDVVGRMGVEEFFPGEFRRGLVWPIPAKSGYVRALRRCRGFIRLP
                     EDRETVRAGEEVEVCLW"
     gene            complement(2057..>2260)
                     /locus_tag="PL162_08995"
     CDS             complement(2057..>2260)
                     /locus_tag="PL162_08995"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HQF92192.1"
                     /translation="AFTERFQADALGAKARQQGYTVVVVSATVNGKQYFRVRVVAGSS
                     KGSAEELQEKLKKEGYPTIVVRL"
     assembly_gap    2261..2360
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2361..3557)
                     /locus_tag="PL162_09000"
     CDS             complement(<2361..3557)
                     /locus_tag="PL162_09000"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQF92193.1"
                     /translation="MSETRGRHRGAGSSGHYGEWCTVPWRRGGDPLWALLFPAPYAVG
                     MANLGVQLVAYGLQKLGVGVERFFLSLPPLSSLESGRPLRDFPIITASVAFEPDVLAF
                     FRILKDHRIPLSWKDRRDGPYPLIGVGGALCSLNPTLFQEVADFILVGDSEPELPYLV
                     AALRAYLRHGDRDRLWEELDRRPVIAVPPRERLDKDLCRSVCFDLDEHGGHSVWVAPK
                     SVFGGTFLMELQRGCVRGCPYCTLPSSFSPRRVRGIDGVREMLSRAAGKVSQVGLITP
                     EAGDYPHMSPLLEELRRHSLGVSFASLRVDALTEEMMISLTESQRSSVTIAPETSERL
                     RKRCGKSFSDALIMEKLHMARRWGIHKVKLYYMTGLPEERDEDLEAMAQLAASIVRDV
                     GLDVVVG"
     gene            complement(3580..4764)
                     /gene="ftsZ"
                     /locus_tag="PL162_09005"
     CDS             complement(3580..4764)
                     /gene="ftsZ"
                     /locus_tag="PL162_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HQF92194.1"
                     /translation="MSDVFEMEPFRRQYREIIKVVGVGGGGNNALNHIIRNGVSGVEF
                     IAANTDVAHLERSEAKSRIILGAELTKGLGAGADPEVGLNAAKESLEEIKAAIGGADM
                     VFLTAGMGGGTGTGATPVIAEAAKDMGALVVAVVTRPFQFEGKRRGNQAAAGIQKLKE
                     KVDALIVIPNDRLLQMADKNTPLSAAFNLADEVLRQAVQGVTDLVLRPGLVNVDFADM
                     RTVMRNAGSAIMGIGEGRGDDRAAMAARTAINSPLMESTMHGAKGVLFNVTGGPSLGI
                     LEIQQAAAVITDAADEDASIIWGHVLDPSMDDTVSITVIATGFSGVVGEGAVKGRGGL
                     RGRMELEEADTRLSTREEDMFVVPTLPPSEIDKSPILRGENKTPPQVIRRPRGGRNDQ
                     RS"
BASE COUNT          946 a         1639 c         1321 g          829 t
ORIGIN      
        1 aggatcctcc cctgagatga caacaccgag gccgcatgat ctctgtcccc tttggccgtg
       61 cccgccccca ggaccaccac gtcggccccc gcacaggcat cccgaagagc ctgagccaac
      121 agagcgggat catcggccac cacaggggcc acggcgagtt gaacccccca gcgattcaga
      181 taccccttga ggaggaggga gttcgtctct cccaccaacc cctctgaggg cgaggccgtg
      241 gccagccacc gatcgggagc gatgatctcg tcccctgtgg ggatataagt caccctcatc
      301 cgaggtcgca ccggaagggt tcccactccc gccgccacgc agagggccgc cagggccggg
      361 gtaacccggt ctccctgacg agcgatcagg cggcgggcca tggcatcctc tcccaccgct
      421 cgaacgttca tgcccggggg aagggcccgg tgaacccgaa tgcccccaga ggcaggatct
      481 tccgtcacat cttccaccat gaccacggca tcgtaggggg aggggacggg agcccccgta
      541 ttacaccaca cggcgtcgtt tcctccaacg aacaccgggg aagcgggaga agctcctccc
      601 agggcctcag atcgcaacgc aaagccatcc accgccgaag cgcgaaagtg agggacgttc
      661 cgctgcgcca ccacatcgcg cgtcagggga cggtccaagg cctcctccgg agagcaccac
      721 gcctcgtcgc gtcgcaagac tggtcccact tcccgacgca atatggacca gcactccggc
      781 aacgtaatcc actggggggg ggcttttacc acaggcatac ctccacctcc tctcccgcac
      841 ggaccgtctc ccggtcctcc ggaagccgaa taaacccccg acatcgtcgt aaggcccgga
      901 catatcccga cttggcgggg atgggccata cgaggcccct tcggaactcc cctgggaaaa
      961 actcttccac tcccatcctc cccaccacgt cgtcccccgt gcggacgagg atcggcgagt
     1021 cctccccctc gccatctccc cggagcatgt gcaccagggg caccaccacc gtcaaggcca
     1081 ccaccgcgca ggaaaggggg tgccccggta gccccaccac cagacggaaa ggagtacggg
     1141 accctccaat cagcgtaggt ttccccggag aaaggttgag ccccctcacc aagagtcccg
     1201 ggtcggaaat ggtccgaagg aggtccaggc aaaggtcccg ggtgctcacc gacgatccac
     1261 cggaaagaag gaccacgtcg ttttccgact ccgcccggcg aaagacctcc tggagcgtgg
     1321 cgggaacgtc ggggacaatg ccgtaggccg tgacgtggca tcccatacgc cgaaagagga
     1381 gcgtcagaaa agtttcgtta gcgtctcgca cgcaccccag aggtagaggg aaggtctccg
     1441 caggaacaat ttcatccccc gtggacagga tgcccaccct caggcaggag acctccaggg
     1501 ccgtaatccc cagggccgcc agggcgaggg agactccggg gctcacccct tctccccgat
     1561 ctaataccac actttgggcc gagacgtctt cccctgcctt tacgataaaa tccccgggcg
     1621 cggcggcacg gcgcacctcc aggaggtttc ccatcagcgc ggtgtcttcc gccatgatca
     1681 cggcatcata cccctcgggc acgcctccgc cggtgtaaag ggcggcggcg gatcccgagg
     1741 tgccagaaaa agagggaaga ctccccatgg ggacttcccc tttcacggaa agaaacgccg
     1801 gggacgaggg ggaagcccct cgcacatcca cgctccttac ggcataaccg tcccgcaagc
     1861 ttcggtcgaa agctggcacc ggctccgggg cgcggacaaa acgagccgcc gcccctccct
     1921 ggacctccaa gagaggtttt ttctcggagg ggagatccca gggaaaggag agcgcctcgg
     1981 ccacgcgccg gagcgcttcc cgacgtgcgg taagatgttc gacaaaaccg gccatggggt
     2041 gtcctttctc cccttttcag aggcgcacga cgatcgtggg atatccttct tttttcagct
     2101 tttcttgaag ctcttccgcc gatcccttgc ttgaccccgc caccactcgc accctaaagt
     2161 actgcttccc gttcaccgtc gccgagacca ccaccacggt gtatccctgt tgtcgcgcct
     2221 tcgcccccag ggcgtccgcc tgaaagcgct cggtgaaagc nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn ccccaccacc acgtccaacc ccacgtcccg gacgatggac
     2401 gcagccagct gagccatggc ttctagatct tcgtcccgtt cctccggaag gcccgtcatg
     2461 tagtaaagct taaccttatg tatgccccat cgacgggcca tgtggagctt ttccattatc
     2521 aaggcgtccg agaaagattt tccacagcgc ttccgcaggc gctcggaggt ctctggcgcg
     2581 atggtcacac tggagcgctg gctttccgtg agggagatca tcatctcctc ggtgagggcg
     2641 tccacccgga gggacgcgaa ggaaaccccc aggctatggc ggcgaagttc ttccaaaagc
     2701 ggcgacatgt ggggatagtc ccccgcctcg ggagtgatga gccccacctg ggaaactttc
     2761 cccgccgccc gggaaagcat ctcccgcact ccgtcgatcc ctcgaacgcg acggggagag
     2821 aagcttgaag gcagcgtgca gtaagggcac ccccgcacac acccccgttg gagttccatc
     2881 aaaaacgttc cgccaaagac gctcttgggg gcgacccaga cgctgtgtcc tccgtgttcg
     2941 tcaagatcga agcagacgct ccgacacaaa tctttatcaa gtctctcccg gggaggaacc
     3001 gcgatgacgg gacgtcggtc caactcctcc catagcctat ctcgatcgcc gtggcgaaga
     3061 taggctcgaa gggcggccac caagtagggg agctctggtt ccgagtcccc caccaggata
     3121 aaatccgcca cttcctgaaa cagagtggga ttcagggagc acagggcccc ccccacgccg
     3181 atcaggggat aggggccgtc tcggcggtcc ttccaggaca agggaatccg gtgatccttc
     3241 aagatccgga aaaacgccag gacatccggt tcgaaagcca cactggcggt gatgatggga
     3301 aaatctctca ggggacgccc cgattccagc gaagacaaag ggggcagaga gaggaaaaaa
     3361 cgctccaccc ccacccccag tttctgcaac ccgtacgcca ccagctgcac tcccagattc
     3421 gccatgccca ccgcataggg agcgggaaag agcaacgccc aaagagggtc tcccccccga
     3481 cgccaaggca ccgtgcacca ctcgccgtaa tgccccgagg atcctgcgcc gcgatgtctc
     3541 ccccgagtct cgctcaaccg tcaccgctcc ttccgacgat caagagcgct gatcgttgcg
     3601 tccccctcgg ggcctgcgga tcacctgggg aggggtcttg ttttcgcccc tcagaatggg
     3661 tgatttgtcg atttcagacg gggggagggt cggcacgaca aacatatcct cttctctcgt
     3721 ggaaagcctg gtatcggcct cttccaactc catccttccc cgcagtcctc cccgcccttt
     3781 gacggctcct tcccccacca ctccggaaaa ccccgtggcg atgaccgtga tggacaccgt
     3841 gtcgtccatg gagggatcca gcacatgacc ccagatgatg gaagcgtctt cgtctgcggc
     3901 gtcggtgatc accgccgccg cctgctgaat ctccaaaata cccaggctgg ggcccccggt
     3961 cacgttgaac aggactccct tggccccgtg catcgtggat tccatcagag gactattgat
     4021 cgccgtccga gccgccatgg ccgcccgatc gtctcctcgg ccttcgccta tgcccatgat
     4081 agcggatcct gcatttctca tgaccgtcct catgtccgcg aagtccacgt tcaccagccc
     4141 ggggcgaagt accagatctg tcactccttg gaccgcttgg cgcagcacct cgtctgccaa
     4201 gttgaacgcc gccgatagag gggtgttctt gtcggccatc tggagaaggc gatcgttcgg
     4261 tatgacgatg agcgcatcca ctttctcttt gagcttctga atgccagcgg cggcttggtt
     4321 accccgccgc tttccctcga actgaaaggg tcgcgtgacc accgccacca cgagggctcc
     4381 catgtcttta gccgcctcgg cgatgacggg ggtggccccc gtccccgtgc ctccccccat
     4441 gcccgccgtg agaaatacca tgtccgctcc accgatggcg gccttgattt cttccaagga
     4501 ttccttagcg gcgttgagcc ccacctcggg gtctgctccg gctccaagcc ccttggtgag
     4561 ctccgccccg agaatgatcc gggacttcgc ctcggatcgc tccaggtggg ccacgtcggt
     4621 attggcggca atgaactcaa cgccggacac cccattccgg atgatgtgat tgagggcgtt
     4681 gttgcctcct ccccccacac cgacaacttt gattatttca cggtactgac gacggaatgg
     4741 ttccatttca aatacgtccg acatcgatcc aacctcctgc agtgtccggc gctcgaagga
     4801 aatcagaaaa gctcttttag cgttttttta atagc
//
LOCUS       DAOOER010000100         4808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_16796_length_4808_cov_7.651168, whole genome shotgun sequence.
ACCESSION   DAOOER010000100 DAOOER010000000
VERSION     DAOOER010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16796_length_4808_cov_7.651168"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2251
                     /gene="mutS"
                     /locus_tag="PL162_09010"
     CDS             <1..2251
                     /gene="mutS"
                     /locus_tag="PL162_09010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HQF92195.1"
                     /translation="PDEGGSEGRLAAVSGERGALSLALLAPAVGTLEVGTLPEEEALA
                     TLSAFAPQEILVPERPPQALSSRGGLLPLARPLRRDRRSFDPLAGGKALAQRWQIASV
                     ESHGVSAADPAVGCAAAALEYLEETQFTAAAHVARLLPLRRGEQLFLDVTTQKNLELV
                     DGGGATLYGVLHRCRTSMGRRTLEEWILRPSRSMEVIQNRQGAVGWGKDHQDDTRGIQ
                     DLLGRCRDVERSLGRLVLGVAGPRDLGAVRDTLAVLPDLRRRLADTPVERATGPLPDL
                     SSLREELLRALEDDLPRHLREGRVVREGYDQELDEGRRLGDGSEAWLAAFLEGERERT
                     GLRTLKAGYNKVFGYYLEVSRSQAHRVPPTYHRKQTLVGVERYITDELKAFEGRMASA
                     EERVASRERELYEALENRVRDEGGNLQILGKILARTDVLLSLGEVAWERRYVCPRVVP
                     EGALLLKGARHPVVEKALAPDPYTPNDVRLEAHQGRIALVTGPNMAGKSTYLRMAALH
                     VILAQMGSFVPAEEAQVPLADRIFTRIGARDELDRGHSTFMVEMIETASILHNLTDAS
                     LVVLDEIGRGTSTYDGMSIAWAVLEYLERHCGCSPKVLFATHYHELTRLGKGDGVIHN
                     YSMAVEERPDGIVFLHRVVPRPADRSYGIEVARRAGIPDVVVQRARELLETLEKGAPA
                     EEVERIPSSQGRKIPGGGRQLVLGDMTRDALLEELASFKTADITPFQAQELLVKLRRR
                     ARDIVGGPR"
     gene            2248..3963
                     /gene="mutL"
                     /locus_tag="PL162_09015"
     CDS             2248..3963
                     /gene="mutL"
                     /locus_tag="PL162_09015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HQF92196.1"
                     /translation="MIRRLPPEVAVRIAAGEVIERPFSAVKELLENALDAGARRVTIA
                     LISGGAGSLVVEDDGGGIPPEELPLAVERYATSKISEENDLLALRTLGFRGEALASLG
                     AVGRMEIRSRVPGTPDGAFLRLEGGIVVGHGIIPCAPGTRIQVDDLFFNVPARRKFLK
                     GAPAETRRVVGVARDYALAYPAVAFLVLSEGKKIFSSSGEGDRQALLRQLWGSEPETR
                     FAAVHVNSVRLEAFYQPSSGGSAPHLTAFVNGRRFFDPLLRAALASVPSSGGGEWFLS
                     LTVPPGELDVNIHPAKVEVRFRCSRDVFDALREGAKELLADPPTIPLPGKREGPFVTP
                     PIASLPFRPAAMTFDRVASSKGSEGSAPPQECRDQGVLSSLAKPPSDPQDPHEECRYL
                     GQMVAGFLIFEDSHGLIVMDPHAAHERVRYEALGEERGEGAVQTLAWPLPLPPSLGLR
                     VTPLENPLQNLGFLLEEREGGWSLKGFPVHPRVTPPPPLDFLRRVVDSLEREEAPPLE
                     ILWRTWASLACAGAVKLGESMDAAEARALWRHLQQCAHPEACPHGRPTLLRLETTSLT
                     RHFRR"
     gene            3969..>4808
                     /gene="miaA"
                     /locus_tag="PL162_09020"
     CDS             3969..>4808
                     /gene="miaA"
                     /locus_tag="PL162_09020"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HQF92197.1"
                     /translation="MTPFLLALIGPTAVGKTAISLPLAEALGAEIISVDSRQVYRYLD
                     VGADKISPEVRRRIPHHLIDVVDPDEPFSAADFVARTTDAVERIRRRGRIPLLVGGTP
                     FYYAALAEKTLTPDLPRDWSLRARLEEEAQKEGREALYRRLAERDPDYAGRVHPHDLR
                     RVVRGLEILELSGKTPTQIYREGGRKEGAFALSYLGLTRPREELYRGIARRATAQFSA
                     GFPEEVEMLLSRGYDPRCPALQGFGYRELVRYHRGECTLEDALVGDIAATKAFSRRQM
                     TWFR"
BASE COUNT          864 a         1515 c         1575 g          854 t
ORIGIN      
        1 ccccgacgaa gggggaagcg aagggcggct ggcggcggta tcgggggaac ggggagcgct
       61 gtctttggcc ctcctggcgc ctgcggtggg cacgttggag gtggggaccc tcccggaaga
      121 agaggccctg gccactctct cggcctttgc cccccaggag atcctcgtgc ctgagcgccc
      181 tccccaggcc ctctcctccc ggggggggct cttacccctg gcacgtcccc tgcggcgaga
      241 tcgacgatct tttgaccccc tcgcgggggg caaagccctg gcccagcggt ggcaaatagc
      301 ctcggtggaa tcccacggcg tttctgcggc ggatccggcg gtgggctgtg cggcggcggc
      361 gctggagtac ctcgaagaaa ctcagttcac cgccgcagcc cacgtggcaa ggcttttacc
      421 cctgcgccgg ggagaacagt tgtttttgga cgtgacgacg cagaaaaacc tcgagttggt
      481 cgacggaggc ggggccaccc tctacggcgt gctccaccgc tgccgtacct ccatggggcg
      541 tcgcacgctg gaagaatgga tcctgcgccc ttcgcgaagt atggaagtca tccaaaatcg
      601 ccaaggggcg gtgggatggg gtaaagacca ccaggacgat acccgaggaa tccaggatct
      661 tctcgggcgc tgccgagacg tagagcgctc cctggggcga ctggtgttag gggtggcagg
      721 gcctcgggat ttgggggcgg tgcgcgacac cctggcggtg ctccccgatc tccggaggcg
      781 cctggcggac acccccgtgg aacgggcgac gggaccgttg cctgacttat cctccctgcg
      841 agaggaactc ctgcgggccc tggaggacga cctgccccgt cacctgcgcg agggacgggt
      901 cgtacgcgaa gggtacgacc aggagctcga cgaagggcgc cgcctgggag acggaagcga
      961 agcttggctt gcggcctttt tggaggggga gcgagagcgg acgggccttc ggaccctcaa
     1021 agcggggtac aacaaggtgt ttgggtacta cctcgaagtg agccgatccc aggcccaccg
     1081 ggttcctccg acctatcacc ggaagcagac cttggtagga gtggagcggt acatcaccga
     1141 cgaactcaag gccttcgagg ggcgcatggc ttccgccgaa gaacgggtcg cctcgcggga
     1201 gagggagctc tacgaagccc tagaaaatcg cgtacgcgac gaagggggca accttcaaat
     1261 cctggggaag atcttggcac gcacggatgt gctcctatcc ctgggggaag tggcctggga
     1321 gcggcgctac gtatgccctc gggtggtccc cgaaggggcc ctcctgctca aaggagcccg
     1381 ccaccccgtg gtggaaaagg ccctggcccc cgatccctac actcccaacg acgtgcgcct
     1441 cgaggcccat caaggaagaa ttgccctggt gacggggccc aacatggcgg gcaaatccac
     1501 ctatctgcgc atggcggccc tccacgtgat tctggcccaa atggggagtt tcgtccccgc
     1561 cgaggaggcc caagttcccc tggccgaccg gatctttact cgcatcggcg cccgagacga
     1621 gctcgatcgg ggacacagca ccttcatggt ggagatgatt gaaacggcct ccatactcca
     1681 taacctcacc gacgctagcc tggtcgtact ggacgagatc ggccgaggga cttccaccta
     1741 tgacggcatg agcatcgcgt gggccgtgtt ggagtatctg gaacgccact gtggatgctc
     1801 ccccaaggtc ctctttgcca cccactacca tgaactgacg cgcctgggga agggcgatgg
     1861 cgtgatccac aactacagca tggcggtgga agaacgcccc gatggcatcg ttttcctgca
     1921 ccgggtggtg cctcgtccgg cggaccgatc ctatggcatc gaagtggccc ggagggctgg
     1981 cattcccgac gtggtggtac aaagggcccg agagctcttg gagacgctgg aaaagggcgc
     2041 cccggcggaa gaagtagagc gaatcccctc ctcccaaggc agaaagattc cgggaggggg
     2101 gcggcagctg gtgttgggag acatgacccg cgacgcccta ttggaagagc tggcctcctt
     2161 caagacggcg gatatcaccc cctttcaagc acaggagctc ttggtcaagc ttcgccgccg
     2221 ggcccgagac atcgtgggag gccctcggtg atccgccgct tgcccccgga ggtggcggtg
     2281 cgcatcgccg ccggagaagt cattgaacgc cccttctccg ccgtgaagga gctcctggaa
     2341 aacgccctgg atgcgggggc ccggcgggtg accatcgctt tgatctccgg gggcgcgggg
     2401 tctttggtgg tggaagacga cggcgggggg atccccccgg aggaactccc cttggctgtg
     2461 gagcgatatg ccactagtaa aatttccgaa gaaaatgacc tcctggccct acgcaccctg
     2521 ggctttcgag gggaggccct ggccagcttg ggggccgtgg ggcgcatgga gatccgcagc
     2581 cgcgtccccg ggacccccga tggagccttt ttgcgcctgg aaggggggat cgtggtggga
     2641 catgggatta tcccttgcgc cccggggacc cgtatccagg tggacgacct gttcttcaac
     2701 gtaccggccc gacggaagtt tctgaaagga gccccggcgg aaacccgacg cgtggtcgga
     2761 gtggcccggg actatgcctt ggcctacccc gccgtagctt ttttggtcct ttccgagggg
     2821 aaaaagatat tctcctcctc gggagaggga gaccggcagg cccttctacg ccaactctgg
     2881 ggatcggagc ccgagacacg cttcgcggcg gtgcacgtga actccgtccg cctggaggcc
     2941 ttttatcagc cctcctccgg aggatcggca ccccacctta cggccttcgt caacggccga
     3001 cgcttctttg accctttgct ccgggccgcc ctggcctctg tgccaagctc cggaggagga
     3061 gagtggtttt tatccctgac cgttcctcca ggggagctgg atgtaaacat ccacccggcc
     3121 aaggtggagg tgcgattccg atgttctagg gacgtcttcg atgccctgcg ggagggcgca
     3181 aaagagctcc tggccgatcc tcccaccatc ccgttgcccg ggaagcgaga gggccccttc
     3241 gtgacccctc ccatcgcctc cctccctttc cgacctgcgg cgatgacctt cgatcgggtg
     3301 gcttcttcca aagggagcga aggaagtgcc cctccccagg aatgccgaga tcaaggcgta
     3361 ctttcctcac tggcgaaacc tccctccgac ccccaagatc ctcatgaaga atgccggtat
     3421 ctgggtcaga tggtcgccgg atttctgatt ttcgaagatt cgcacggact aatcgtcatg
     3481 gatcctcatg cggcccatga gcgcgtccgg tacgaagccc tgggggaaga acgaggggaa
     3541 ggagcggtcc aaaccttggc gtggcccctg cccctacccc cttccttggg gctccgggtg
     3601 acgcccctgg aaaaccccct acaaaacttg ggctttttgc tggaagaaag agagggggga
     3661 tggagtctta aaggatttcc cgtgcatccc cgggtgaccc ctccgccacc cctggatttt
     3721 ttacgccggg tggtggactc cctcgaacgg gaggaagccc ctcccctaga gatcctttgg
     3781 cgaacctggg cttcccttgc ctgcgcgggg gcggtgaagt taggggaatc catggatgcc
     3841 gccgaggctc gggcgctgtg gcgccacctt cagcagtgcg cccatccgga agcatgcccc
     3901 cacggacggc ccacccttct ccgcctagag acgacgtctt tgacccgcca ttttaggagg
     3961 tgacgccctt gaccccattc cttttggccc tcatcggccc cacggcggtg gggaaaaccg
     4021 ccatcagcct ccccttggcg gaggccctgg gggcggagat catctccgtg gattcccgcc
     4081 aggtctaccg ctatttggat gtgggagcgg acaagatctc ccccgaggtg cggcgccgca
     4141 tccctcacca cctcatcgac gtggtagatc cggacgagcc cttttcggcg gcggatttcg
     4201 tggcgcggac caccgacgca gtggagcgca tccggcgccg ggggaggatc cccctcttgg
     4261 taggagggac gcccttttat tacgcagccc tggcggaaaa gacccttacc ccggacctac
     4321 ctcgggactg gagccttcgc gcccgtctgg aggaggaggc tcaaaaagaa ggccgggaag
     4381 ccctctaccg gcgactggcc gagcgagacc cggattatgc cggacgcgtc cacccccacg
     4441 atctacgacg ggtggtgcgg ggactggaga tcttggagct ctcggggaaa acccccaccc
     4501 agatttatcg cgaagggggg cgaaaagagg gggcctttgc cctttcctac ctgggattga
     4561 cgcgccccag ggaagagctg taccggggta tcgcccgccg agcgaccgcc caattttcgg
     4621 cgggctttcc ggaggaagtg gagatgctcc ttagccgggg atacgacccc cgatgccccg
     4681 ctctgcaagg ctttgggtat cgagagctcg ttcgatatca ccgaggagaa tgcaccttgg
     4741 aggacgcctt ggtgggagac atcgccgcca caaaggcctt ttctcgaaga caaatgacgt
     4801 ggtttcgt
//
LOCUS       DAOOER010000101         4707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_17244_length_4707_cov_8.509458, whole genome shotgun sequence.
ACCESSION   DAOOER010000101 DAOOER010000000
VERSION     DAOOER010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17244_length_4707_cov_8.509458"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PL162_09025"
     CDS             <1..300
                     /locus_tag="PL162_09025"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQF92198.1"
                     /translation="INEGVSNAFKHGTLPGEPPWVALTLTEEGPGVVATIQDRGPGLP
                     PGALQKDFPDLDQEGGRGLPLMVRLADEVSYDAATRTLRLVARRPLLSPARDGVS"
     gene            313..618
                     /locus_tag="PL162_09030"
     CDS             313..618
                     /locus_tag="PL162_09030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HQF92199.1"
                     /translation="MEYRFEPLGNTLHLTLQGSMYVDDAAKLREDALASLERGACSRV
                     EADLSRLDYIDSAGLGVLISLHKRCARKGGSLVVRGLSGNVKELFEITRMDKVFLQG"
     gene            658..963
                     /locus_tag="PL162_09035"
     CDS             658..963
                     /locus_tag="PL162_09035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HQF92200.1"
                     /translation="MTYNIDLRGQTLHVELRGSMYAGDATAFRRASKAALEGRRCSRV
                     EVDLSHLDYIDSAGLEVFISLHKRCVKEGGSLAVRGLSGPVKELFEITRLDKVFLQE"
     assembly_gap    1173..1272
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1298..3208
                     /gene="gyrB"
                     /locus_tag="PL162_09040"
     CDS             1298..3208
                     /gene="gyrB"
                     /locus_tag="PL162_09040"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HQF92201.1"
                     /translation="MTFEDPSKQYTAKDIQVLEGLEAVRKRPGMYIGDTASRGLHHLV
                     YEVVDNSLDEALAGFCSEVEVTVYSDQSVSVRDNGRGIPVDLHPQMEKSAAEVVLTVL
                     HAGGKFDKKSYQVSGGLHGVGVSVVNALSARLSLTIWRGGHEWRQEYRRGVPTAPLAA
                     AGDAEGSGTLIHFLPDPDIFSETEFSWDILSSRLRELAFLNPGIRIVLRHIPLEKEKT
                     FRYEGGTCSFAEYLNRGRETLFSPPLCISGERDGVMVDVAIQYNDSYLERVFGFANLI
                     HTIEGGTHVSGFRTALTRGVNEMARRMKIMKEKDPNLTGDDLKEGLTAVLSVKLPEPQ
                     FEGQTKTKLGNSEIKGIVDSIVYDGLLALLEERVEVLRPLVEKAVLARQAREAAKKAR
                     ELVQRKGALSSFDLPGKLADCSGKDPAACEVYIVEGDSAGGSAKQGRDRSFQAILPLR
                     GKILNVEKARLDKALSNLEIRTIIRALGCGIGDDFDAAKLRYHRTIIMTDADVDGAHI
                     RTLLLTLFYRFMPQIIERGHLYVAQPPLYRFQSGKDVTYCYTEKELKELIARGEGKKY
                     TIQRYKGLGEMNPEQLWETTMNPATRTMKRVAVEDAVEVDEAFSILMGDKVEPRREFI
                     EQHAREVRNLDV"
     gene            complement(3376..4155)
                     /locus_tag="PL162_09045"
     CDS             complement(3376..4155)
                     /locus_tag="PL162_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92202.1"
                     /translation="MGVGMGMKAWFEGLRGKFSEKGSPPSSEDPPLPGDGSPEDPDPF
                     PPPTAPPTVAAEAPGASGGVLGALFQRRRREDRSRRRRAWGKTLILVALLGGGLLWVL
                     FRPREEALPGPDLRRATPLDEMLYAPLGVPLVTMAYRDLVTQGVRQTVPWFPGARELR
                     ASGDVEQFSARGMDVLFLKVSGDLFPLALRVTGKERAVKWGLRPGTDRRDVARRLGAP
                     QRASEDVWAYDDSWGSEVCFYFQGDRVWAVEWHYPPGLRLP"
     gene            complement(4179..>4707)
                     /locus_tag="PL162_09050"
     CDS             complement(4179..>4707)
                     /locus_tag="PL162_09050"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HQF92203.1"
                     /translation="PQGVAPEVLRRIFEAARWAPSAYNEQPWRFLVARREEEEGFNAL
                     LGCLVKANRLWACRAGALAVGIVKRALDYDGKANLWAEHDLGAAVAQLSLQAVAEGLF
                     VHPMAGFSPSRVRESCEVPEGYDPLVALAMGYGGDPDDLPEELALREKEPRERRPLEE
                     MVFGARWKTEAPWGR"
BASE COUNT          853 a         1584 c         1458 g          712 t
ORIGIN      
        1 atcaacgagg gggtctccaa cgccttcaag cacggcaccc tcccgggaga gcccccctgg
       61 gtggccctga ccctgacaga agaggggcct ggggtggtgg ccacgatcca agaccggggg
      121 ccgggcctac cccccggcgc cctacaaaag gatttccccg acctcgacca ggaagggggg
      181 cgggggttgc ccctcatggt gaggctggcg gatgaggtgt cctacgatgc ggcgacccgc
      241 acgttgcgcc tggtggcgcg acgacccctt ctctcccctg ccagagacgg cgtatcttaa
      301 ggaggaatga tcatggaata ccgctttgaa cccctgggaa acaccctgca cctgacgctc
      361 cagggctcca tgtacgtgga cgacgcagct aaactccggg aggacgccct ggcctccctg
      421 gagagagggg cctgctcccg ggtggaggcc gacctttccc ggctcgacta tatcgacagc
      481 gcggggctcg gggtcctgat ctcgctgcac aagcgctgcg cccgtaaggg gggcagcctg
      541 gtggtgcgag ggctctccgg caacgtgaag gagctcttcg agatcacccg catggacaag
      601 gtcttccttc agggataatg cctcccctcc tgccccccca ccccagaagg aagggccatg
      661 acctataaca tcgacctccg gggacagacc cttcacgtgg agctccgggg ctccatgtac
      721 gccggggatg ccacggcatt tcgcagggcc tccaaggccg ccctggaggg gcggcgttgc
      781 tctcgggtgg aggtggacct ttctcacctg gactacatcg acagtgcggg gttagaggtt
      841 tttatttctc tacacaagcg ctgcgtaaaa gaaggaggtt ccttggcggt gcgagggctc
      901 tcgggccccg tgaaggagct ctttgagatc acccgcctgg acaaggtctt ccttcaggag
      961 tagccccgta gggctcatcg ccccaggcct tcccggggaa aaataatagc cccctcaccg
     1021 gaaggagagc gccccaggat ctgcggcggc cccggggacc tgaggccacc tccccaaagg
     1081 agagaagaga tgtttttacc gctggaagaa aacgacctgg tgcacatggg tcgggtcgtg
     1141 gctctggtgc gcgacgatgg agagacggcc gtnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnttcccgcc aaaggaggcc cctgcgtatg acgtttgaag acccatctaa
     1321 gcagtacacc gccaaagaca tccaggtcct ggaggggctg gaagcggtac ggaagcgccc
     1381 cgggatgtac atcggcgaca ccgcctcccg ggggctccac cacctggtat acgaagtggt
     1441 ggacaactcc ctggacgagg ccctggcggg cttttgctcc gaggtggagg tgacggtgta
     1501 ctccgaccag agcgtctccg tccgggacaa cggccggggc attcccgtgg acctccaccc
     1561 ccagatggag aaatccgccg ccgaagtggt gctcaccgtg ctccacgccg ggggaaagtt
     1621 cgacaagaaa tcctaccagg tctccggggg gctccacggc gtgggagttt ccgtggtgaa
     1681 cgccctctcg gcccgccttt ccctcaccat ctggcgaggg gggcacgagt ggcgccagga
     1741 ataccgccgg ggggtgccca cggcgcccct cgccgccgca ggcgacgccg agggctccgg
     1801 caccctgatc cacttcctcc cggatccgga catcttctcg gagacagagt tttcctggga
     1861 catcctgagc tcccgcctcc gggagctggc cttcctgaac ccggggatcc gcatcgtctt
     1921 gcgccatatc cccctggaga aggaaaagac cttccgctac gaggggggca cctgctcctt
     1981 cgcggagtac ctgaatcgag gccgggaaac gctcttttct ccccccctgt gcatcagcgg
     2041 agaaagagac ggcgtcatgg tggacgtggc catccagtat aacgactcct atctggagcg
     2101 ggtcttcggc tttgccaacc tcatccacac catcgagggg ggcacccacg tctcgggctt
     2161 tcgcaccgcc ctcacccggg gggtgaatga gatggcccga cgcatgaaga tcatgaagga
     2221 aaaagacccc aacctcaccg gggacgactt gaaagagggg ctcacggcgg tgctctccgt
     2281 gaagcttccc gagccccagt tcgaggggca gaccaagacc aagctcggca acagcgagat
     2341 caagggcatc gtggactcca tcgtctacga cgggctcctg gcattgctgg aggagcgggt
     2401 ggaggtgcta cgccccctgg tggagaaggc cgtactggcc cgccaggctc gagaggccgc
     2461 caagaaggcc cgagagctgg tgcagcgcaa aggggccctc tcttccttcg acctgccggg
     2521 caagctcgcc gactgctcgg ggaaagaccc tgcggcgtgc gaggtctaca tcgtggaggg
     2581 agacagcgcc gggggctccg ccaagcaggg gcgggaccgg agcttccagg ccatcctccc
     2641 cctacggggc aaaattctca acgtggaaaa ggcccgcctc gacaaggccc tctccaacct
     2701 cgagatccgc accatcatcc gggccctggg gtgcggcatc ggagacgact tcgacgccgc
     2761 caagctccga taccaccgca ccatcatcat gaccgacgcc gacgtggacg gggcccacat
     2821 ccgcacgctg cttttgacgc tcttttatcg attcatgccc cagatcatcg aacggggaca
     2881 tctctacgtg gcccagcccc ccttgtaccg ctttcagtcc ggcaaggacg tgacctactg
     2941 ctacaccgaa aaggagctca aggagctcat cgcccgtggc gaggggaaaa agtacaccat
     3001 ccagcgctac aaagggctgg gggagatgaa tcccgaacag ctctgggaga ccaccatgaa
     3061 ccccgccacc cgcaccatga aacgggtggc cgtggaagac gcggtggaag tggacgaggc
     3121 cttcagcatt ctcatggggg acaaggtgga acctcgaagg gagttcatcg aacagcacgc
     3181 ccgggaggtg cgaaacctgg acgtgtaggg tacggggaac gacggagtcc tccatccttt
     3241 cgcgccatcg agaggcaaaa ccccccgtcg ctttcttcgc cgcctgcggc ccccggcttt
     3301 cccgaggccc gcaggcggcg aaagctttgg cgcgaagagg tcccgggggc ccggggggat
     3361 cgcaccgccc ccaccctagg ggaggcgcaa ccccggaggg tagtgccact ccacggccca
     3421 gacccggtcc ccctgaaagt aaaagcacac ctcgctcccc caggagtcgt cgtaggccca
     3481 gacgtcttcc gacgcccttt ggggggcgcc gaggcgtctc gccacgtccc tccggtcggt
     3541 ccccggcctc aagccccact tgaccgccct ctccttcccc gtcacccgca acgccaaggg
     3601 aaagaggtct ccggagacct tcaggaagag aacatccatc ccccgggcgc tgaactgctc
     3661 cacgtccccc gaggcccgga gttctcgcgc cccgggaaac cagggcaccg tctggcgcac
     3721 cccctgagtg accaggtcgc gatacgccat ggtgaccagg ggcaccccca ggggggcgta
     3781 gagcatctcg tccaggggag tggcccggcg aaggtcgggc cccggcaatg cctcttcccg
     3841 gggacgaaac aggacccaga gaagcccccc tcccaggagg gcgacgagga tcagggtctt
     3901 tccccaggcc cgacggcgcc gggaccggtc ctcccgccgc cgtcgttgaa agagggcccc
     3961 cagcaccccc ccggacgccc ccggggcctc cgctgccacc gtcgggggcg ccgtcggggg
     4021 aggaaaggga tcggggtctt ccggggaccc gtcgccggga aggggaggat cctcggagga
     4081 aggaggggat cctttctctg agaacttgcc ccgaagccct tcaaaccacg ccttcatccc
     4141 catgcccaca cccatacccc tctcttcgcc ctgtgggctt aacgccccca gggggcttcc
     4201 gttttccacc tcgccccgaa gaccatctcc tccagggggc gccgctcccg gggctccttt
     4261 tcccggaggg cgagctcctc gggcaggtcg tcgggatctc ccccatagcc catagccagg
     4321 gccaccaagg ggtcataccc ctcggggacc tcgcaggact cccgcacccg ggagggggaa
     4381 aagcccgcca tggggtgcac gaagagccct tcggccaccg cttggaggga gagctgggcc
     4441 accgccgccc ccaggtcgtg ctccgcccag aggttcgcct tgccatcgta atccagggcc
     4501 cgtttgacga tccccaccgc cagggccccg gcgcggcagg cccagaggcg gttggctttc
     4561 accagacacc ccagcagggc gttaaatcct tcctcttcct ctcgccgggc taccaggaat
     4621 cgccagggct gctcgttgta ggcgctcggc gcccaccggg ccgcctcgaa gatccgccgc
     4681 aacacctccg gagccacccc ctggggg
//
LOCUS       DAOOER010000102         4569 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_17889_length_4569_cov_7.685423, whole genome shotgun sequence.
ACCESSION   DAOOER010000102 DAOOER010000000
VERSION     DAOOER010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17889_length_4569_cov_7.685423"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..693
                     /locus_tag="PL162_09055"
     CDS             103..693
                     /locus_tag="PL162_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HQF92204.1"
                     /translation="MRGELLLHCCCAPDGTVPQEVLAREGWGVTLHFFGGNIHPRGEY
                     LRRAEAVRALGRARGVPVVLDPYEPEAWFVAVAPWAGEPEGGRRCLRCFALQLAAAGA
                     AAARQGAEALGTTLTISPHKDPALVNALGERVAASFGLAWEPRVWRRGGGFARSVEES
                     KRLGLYRQNYCGCAYSRRKTSSGGLEGAFPFTPGGF"
     gene            710..1699
                     /locus_tag="PL162_09060"
     CDS             710..1699
                     /locus_tag="PL162_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HQF92205.1"
                     /translation="MAEGLGASPSGKLPPEDLEARVLRYVGAPRDEVIIGPGVGEDAA
                     VIDWPPGRFLVASSDPIVGASRGAGRLLVHVNANDVAAKGGDPAYLLVTLILPRSWTG
                     SQVEGLMKEVDEACRELGVAVVGGHTEFSDRYRDPVLCGTLLGPAERVLSAQAIRPGD
                     VVVVTKHVGLEGMTILAADRPDLLGPVLGDEGLREVSPWSSSVSVVPEARILRPWCRF
                     MHDPTEGGFMGGMDELCRLAGLGVRLDMGRIPLHPATRKAARALGFDPLRLISSGVLL
                     AVIPPEHVERALGALGASGIPAAAVGTMDEGGPRDFGMREELWRLLALPAPGR"
     gene            1770..2870
                     /locus_tag="PL162_09065"
     CDS             1770..2870
                     /locus_tag="PL162_09065"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HQF92206.1"
                     /translation="MPAEHVWKRVEGVREALGKSDAEALVLFVFEGANWESMYYLTGF
                     RGTSSAAVVTKKDAFLITDGRYLSQAQLQSPFTIVPQGQRHIHETVGEVLAGAGVTQA
                     GFEGERISFAFHKKLSDMPVRWHDLSEVLPKLRRCKDLLERQFVEKAASIAGEAFLRT
                     LAQVTPGMTERRVAALLEFAMKDLGAEGGWEDEFIVASGPRNALPHGRASDRILQAGE
                     WVTVDFGAQYGGYVCDLTRNFSLGAPDPWARDLHALLLEAQRSGVRALRHGASGRDVD
                     AAARRVIEDAGFGGAFSHGLGHGFGLHIHEAPRLSYMSEDTLASGDLVTVEPGVYFEG
                     RGGMRVEDDYWVTDLGAVCLSPNLSQEFFVVS"
     assembly_gap    3055..3154
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3155..4147)
                     /gene="pheA"
                     /locus_tag="PL162_09070"
     CDS             complement(<3155..4147)
                     /gene="pheA"
                     /locus_tag="PL162_09070"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HQF92207.1"
                     /translation="MMEKTPPELDALRGDIDRLDDQIAALLRRRLEVARRIGEAKKGA
                     AIFDAAREASVRRRVQEILSDVPSASVHALWREIFALCRDIQRPLRALCLGPDGSFSS
                     EAARRALGAEISLAFCPDISEVFRRVERGDGDLGVVPIENSVEGAVLATLDAFATSSK
                     ELRIQQELSLEVRPGLASRSESLEEVREVLSHPQALAQCRQWLRTHLPGVPLVAAEST
                     SAAAERAARTPEVAAICSPRAMELHGLNLLADAIQDRPDNTTRFWFVGRGTPRATAWD
                     KTSLLFNVRHEPGALFRALEPLHRGGRNLTHIQSRPLPGNPFEYLFFVDFVGHEE"
     gene            complement(4144..>4569)
                     /locus_tag="PL162_09075"
     CDS             complement(4144..>4569)
                     /locus_tag="PL162_09075"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HQF92208.1"
                     /translation="NLNLLGEREPAIYGAVTLDDIESACRAWGEEHGVPVCCFQSNAE
                     GALLDFLQAERHRASGAVLNAAAYTHTSVALHDGVRALPFPVIEVHLSNVAAREPFRA
                     VSLIAPVARGTITGMGALGYILALEALRTMTADAPEERP"
BASE COUNT          635 a         1396 c         1728 g          710 t
ORIGIN      
        1 tccgtgtccc ccgaggcggc ccaggcctta ccctgggagg accgtcccgg ggaggtcccg
       61 gcggaagctc cggaggtccc ccggccggag gaggggtgat ccgtgcgagg ggagctcttg
      121 ctccactgtt gctgcgcccc ggacggcacc gtgccccagg aggtcctggc gcgggagggg
      181 tggggggtga cgttgcactt tttcggcggc aacatccacc cccgggggga atatctccgg
      241 cgggcggagg ccgtccgggc cctggggagg gcgcgcggag tgccggtggt gctggacccc
      301 tatgagcccg aggcgtggtt cgtcgccgtg gctccctggg cgggagagcc cgaggggggg
      361 cggcggtgcc ttcgctgctt cgcccttcag ctggcggcgg cgggggcggc ggcggcgcgc
      421 cagggggccg aggccctggg gaccaccctc actatcagcc cccataagga tcccgccctc
      481 gtcaacgccc tgggggagcg cgtggcggcg tcctttggcc tcgcctggga gccccgggtg
      541 tggcgtcgcg gcggggggtt cgcccgttcc gtggaagaaa gcaagcgcct ggggctttac
      601 cggcaaaact actgcggctg cgcctacagc cggagaaaaa cgtcctctgg ggggttggag
      661 ggggcattcc ccttcactcc tggagggttt taaaggagga gacgatacga tggcggaagg
      721 tcttggggcg tcgccctcgg gcaagctgcc tccggaggat ctggaggccc gggtcctgcg
      781 ttacgtcggc gctcctcggg acgaggtcat catcggcccc ggggtggggg aagacgcggc
      841 ggtcatcgac tggcccccgg ggaggttttt ggtggcgtcc tcggacccca tcgtgggggc
      901 ctcccggggg gcggggcgcc tgctagtgca cgtcaacgcc aacgacgtgg cggccaaggg
      961 gggtgatccg gcctacctcc tggtgaccct catcctcccc cggagctgga cgggaagcca
     1021 ggtggagggg ctcatgaaag aggtcgacga ggcctgccgg gagctcggcg tggcggtggt
     1081 gggggggcac acggagttct ccgaccgcta ccgggacccg gtgctctgcg gcacccttct
     1141 gggaccggcg gagcgggtgc tctccgccca ggccatccgt ccgggggatg tggtggtggt
     1201 gaccaagcac gtgggcctcg agggcatgac catcctggcg gcggatcgac cggacctttt
     1261 ggggcccgtc ctgggggacg agggccttcg tgaggtctcc ccatggtcct cgtcggtgtc
     1321 cgtggtgccg gaggcccgga tcttgcgccc ctggtgccgt ttcatgcacg accccaccga
     1381 ggggggcttc atggggggga tggacgagct gtgccgcctg gcgggcctgg gcgtgcggct
     1441 ggacatgggg cggatccccc tccaccccgc cacccggaag gcggcccggg ccctgggctt
     1501 tgaccccctg cggctcatct cctcgggggt gctcctggcg gtgattcctc cggagcacgt
     1561 ggaaagggcc ctgggggccc tgggggcctc ggggatcccg gcggcggcgg tggggactat
     1621 ggatgaaggg ggtccccggg atttcggcat gcgggaagag ctttggcgcc tcttggccct
     1681 accggccccg gggcgatgag gaggagggcc ccggggcgaa gagggccccg gcgatgcctt
     1741 agcgagacaa aactcaagga ggtcatgtca tgccggcgga gcacgtatgg aagcgggtgg
     1801 agggcgtgcg ggaagccctc gggaagagcg acgcggaggc gctggttctt ttcgtcttcg
     1861 aaggagccaa ttgggaaagc atgtactacc tcacgggatt ccgggggacc agctcggcgg
     1921 cggtggtgac gaaaaaggac gcctttctca tcaccgacgg gcgctacctc tcccaggccc
     1981 agctgcagag cccctttacc atcgttcccc agggacagcg ccacatccac gagaccgtgg
     2041 gagaggtgct ggcgggggcc ggggtgaccc aggcggggtt cgaaggggag aggatctcct
     2101 tcgccttcca caagaagctt tcggacatgc cggtgcgctg gcacgacctg tcggaggtct
     2161 tgccgaaact ccgccggtgc aaggacttgc tggagcgcca gttcgtggaa aaggccgcct
     2221 ccatcgcggg ggaggccttc ctgcggaccc tggcccaggt gactccgggc atgacggagc
     2281 gccgggtggc ggcccttctg gagtttgcca tgaaggatct gggggccgag gggggatggg
     2341 aggacgagtt catcgtggcc tccggccctc ggaacgccct gccccacggc cgggcctcgg
     2401 accgcatcct ccaggcgggg gagtgggtca cggtggattt tggggcgcag tacggggggt
     2461 atgtctgcga tcttacccgg aacttctccc tgggggcccc cgacccctgg gcccgggacc
     2521 tgcatgccct gttgctggag gcccagcgat cgggggtccg ggccctgcgt cacggcgcct
     2581 caggccggga cgtggatgcg gcggcccggc gcgtcatcga ggacgcggga ttcggtggtg
     2641 ccttttccca cggcctggga cacggcttcg ggctccacat tcatgaggcg ccccggctct
     2701 cctacatgtc cgaggatacc ctggcctcgg gggacctggt gacggtggaa ccgggggtct
     2761 acttcgaggg gcggggaggc atgcgggtgg aggatgacta ctgggtgacg gatctggggg
     2821 ccgtttgcct ctcccccaac ctttcccagg agttcttcgt ggtgtcctaa aggcgaggtc
     2881 ggggatgact tcggccaaga gcccttcctg ggagggcttc gaaggaatcg ccgcagccct
     2941 gcgacgggag gggtacgtgg tactcctctg cgtcgccccg gaggatcggg aaagcggcac
     3001 gccgccgcgc atcgccgggg cggtgacctt tcgggggagc cccgaggccc cggannnnnn
     3061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnctcctc gtgccccacg aagtccacga
     3181 agaagaggta ttcaaaggga ttgccgggga gggggcgcga ctggatgtgg gtgaggtttc
     3241 gccccccccg gtggaggggc tccagggccc gaaagagggc ccccggttcg tggcgcacgt
     3301 tgaaaaggag ggaggtcttg tcccaggcgg tggcccgggg cgtccctcgg ccgacgaacc
     3361 agaagcgggt ggtgttgtcc ggtcggtcct ggatggcgtc ggccaggaga tttaggccat
     3421 gcagctccat ggccctggga ctgcatatcg ccgcgacctc gggggtcctg gccgcccgtt
     3481 cggcggcggc gctggtgctc tcggcggcga cgagggggac cccgggaagg tgggtgcgta
     3541 gccactgacg gcattgggcc agggcctggg ggtggctgag cacttcgcgc acctcctcca
     3601 gggactccga tcgagacgcg agcccggggc gcacctccag ggagagctcc tgttgaatcc
     3661 ggagctcctt ggaagaggtg gcgaaggcat cgagggtggc gaggaccgcc ccttcgacgg
     3721 agttctcgat ggggacgacc ccgaggtccc catcgccccg ctccacgcga cgaaagacct
     3781 ccgatatgtc ggggcagaag gccagggaga tctcggcgcc cagggcccgt cgggccgctt
     3841 cggaagagaa ggatccgtcg gggcccaggc agagggccct aagggggcgc tggatgtccc
     3901 ggcacagggc gaaaatctcc cgccataggg cgtggaccga ggcggagggc acgtcggaaa
     3961 ggatctcctg aacgcgtcgc cgcaccgagg cctcccgggc ggcgtcgaaa atggcggcac
     4021 cctttttggc ctcgccgatg cggcgcgcca cctccaggcg ccttcgcaac agggcggcga
     4081 tctggtcgtc gaggcgatcg atgtcgcccc gcagggcatc gagttccgga ggagtttttt
     4141 ccatcatggg cgctcctccg gggcgtccgc cgtcatggtg cggagggcct ccagggccaa
     4201 gatgtatccc agggctccca tcccggtgat ggtaccgcgc gccacggggg cgatgaggct
     4261 caccgcccga aagggttccc gggccgccac gttcgacagg tggacctcga tcaccgggaa
     4321 gggcagggcc cgcacgccat cgtggagcgc cacgctcgtg tgggtgtagg cggcggcgtt
     4381 cagcaccgcc ccagaggcgc ggtggcgctc ggcctgcagg aagtcgagca gggccccctc
     4441 ggcgttgctc tggaagcagc acaccggcac tccgtgctct tccccccagg cgcgacatgc
     4501 gctttcgata tcgtcgaggg tgacggcgcc gtagatcgcg ggctcccgct cgccgaggag
     4561 gttcaagtt
//
LOCUS       DAOOER010000103         4548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_17995_length_4548_cov_6.747830, whole genome shotgun sequence.
ACCESSION   DAOOER010000103 DAOOER010000000
VERSION     DAOOER010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17995_length_4548_cov_6.747830"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1115
                     /locus_tag="PL162_09080"
     CDS             <1..>1115
                     /locus_tag="PL162_09080"
                     /inference="COORDINATES: protein motif:HMM:NF014165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92209.1"
                     /translation="LADWDMDLAMPEADGAFRGEVKGWLADTFHHLWSDAAVGRALDE
                     AERGDWPPEERANLFWWRRRFDRITKLPCDFKAREAEAQTRATEAWKRAKEENSFVPF
                     KEPLTALVALAREKAQCLGGGAHPYDALLDEFMPGTSVASCDALFGAVQAPLRALIDG
                     ALELQQTLPDPLSREISFPEEAQKTLAREMVTALGFDLSRGIFTPTPHHPFTTTLGPH
                     DVRLTTHYKTSSLSGVLGALHEGGHGLYEQGLPPERFGEPVGSALDPSVHEAQSRFWE
                     NHVGRSEPFWSFWLPRLRELCHLEERDLPLDRFLRWLNRVGRGPRRLEADEGTYVLHI
                     LVRYDLERDLMAGHLEVADLPDAWAAAYRSTVGAEPR"
     assembly_gap    1116..1215
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1216..3144
                     /locus_tag="PL162_09085"
     CDS             <1216..3144
                     /locus_tag="PL162_09085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQF92210.1"
                     /translation="IAAEGEGAYRQLFDHMLDGFALHEIILDQDGVPQDYRFLAVNPA
                     FERLTGLRRESLLGRTVKEVLPGTERHWIDTYGRVALTGESVTFENISAELGRYFEVT
                     AYSPRRGFFACIFADVTARRRAEEALRISEERLRFALEATSDGLWDADFSTGEVYWSP
                     RAYAILGYTPEEGPMTLNRWEELLHPQDRKAAEELVQSAFSGESEGYTLEYRLAARNG
                     DWRWVMDRGKVVERDPSGKPRRIVGAMADITERKALEEERRVLEKYLQNVVDSMPSIL
                     VGVDVQGRVTQWNAEAERRTGLSRRHALGRPVRECGNFPEETMALLQEAIALRSLQKA
                     ERISLEEGGERRWSDVLVYPLVANGVAGAVVRVDDVTERTRMEEVMVQNEKMMSLGGL
                     AAGMAHEINNPLAGILQGAQNIQRRLSPEMRKNVEVAEECGVSLEALRRYLEARDIFA
                     FLKDIREAGERAADIVRHMLTFSRRGSMTKAPCALKDLLDHTLKIARADFDLRVRYDF
                     QKILLEREDDLHLPSVSCVAAEIEQVMLNLLRNAAQALSASPSTAFPRIRVVTRREGD
                     WAVIEVWDNGPGVPEEKKRHIFEPFFTTKKAGEGTGLGLSVSYFIVVSRHNGWMKVDD
                     APGGGAVFQVFFPLERGD"
     gene            3146..3544
                     /locus_tag="PL162_09090"
     CDS             3146..3544
                     /locus_tag="PL162_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQF92211.1"
                     /translation="MAEPWRVLVVEDDGTVGDTLAAFLEDEGFDVRLVRSGEEALAIL
                     RSGWIPRVGVVDLRLPGMDGRAVVECARVLAPSMHFLVHTGSPGGVVEDVASSMGVDA
                     KDIFIKPVRDLGLLVERLRRSGEGVDEHGA"
     gene            3528..>4548
                     /locus_tag="PL162_09095"
     CDS             3528..>4548
                     /locus_tag="PL162_09095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQF92212.1"
                     /translation="MSMGHDPGGPKHILVIEDDGAVRRHLAAYLEDCGYRVTAAAEGE
                     GGLEMARTQHPDLVITDLHMPGISGFQVLSALAEEFPALPVLVVSGTDLLTEVVEALR
                     RGAWDYLIKPLGEMGVLEHAVERALERAWLLEENRRSREELEEAVARRTQELQTLLTE
                     RTVLLREVHHRVKNNFQVISSLLQIRARSSRHPEVRNFSSDFAQRIRSMALVHDALYQ
                     TLDFARWNFGEYLKHLVRDLARGDGSPGPEVRVEAAPLEVSLDLAVPCALMAHELVSN
                     ALRHAFPEGGGQVIVSLSAGAPGMGHLEVRDDGVGLSQETLDQAEGGMGLQIARILVQ
                     QLSGTL"
BASE COUNT          769 a         1390 c         1558 g          731 t
ORIGIN      
        1 ctggcggact gggatatgga tctagccatg ccggaggccg acggggcctt tcggggcgaa
       61 gtaaagggat ggctcgccga caccttccac catctctgga gcgacgccgc cgtggggcgg
      121 gcgctggacg aggcagaaag gggggattgg ccccccgagg aacgggccaa cctcttctgg
      181 tggcgccgga ggttcgaccg gatcaccaaa ctcccctgcg atttcaaggc ccgggaggcg
      241 gaggcccaga cccgggccac cgaggcgtgg aaaagggcca aggaagaaaa ctccttcgtc
      301 cccttcaaag agcccctgac ggccctggtg gccctggccc gggagaaggc ccagtgcctc
      361 ggcggcgggg cccaccccta cgatgccctc ctggacgagt tcatgcccgg cacttcggtg
      421 gcttcctgcg acgccctctt cggcgccgtg caggcccccc tgagggcctt gatcgatggg
      481 gccttggagc tccagcaaac cctccccgac cccctttccc gggagatctc cttccccgaa
      541 gaggcccaga agacgctggc ccgggagatg gtgacggccc tgggttttga cctttcccgg
      601 gggatcttta cccccacgcc ccaccacccc ttcaccacca ccctaggtcc ccacgacgtg
      661 cgcctcacca cccattacaa gacctcttcc ctctccgggg tgctgggggc gctgcacgag
      721 ggagggcacg gcctctacga gcagggcctg cctccagagc gcttcggaga gcccgtggga
      781 tcggcgctgg acccctccgt ccacgaggcc cagtcccgat tctgggaaaa ccacgtgggg
      841 cggagcgagc ccttctggtc cttctggctg ccccgccttc gggagctctg ccacctggag
      901 gagcgagacc ttcccctgga ccgattcctg cggtggctca accgggtggg gcgtggcccc
      961 cgccgcctgg aggccgacga gggcacctac gtccttcaca tcctggtgcg ctacgacctg
     1021 gagagggacc tgatggcggg acacctggaa gtggccgacc ttcccgacgc ctgggccgca
     1081 gcctaccgga gcaccgtggg ggcagaaccc cgggannnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnatcgc ggcggaagga gaaggggcct accgccagct cttcgaccac
     1261 atgctggacg gctttgccct gcacgagatc attctggacc aggacggagt gccgcaggac
     1321 tatcgcttct tggcggtgaa tccggccttc gaacgtctca cggggttacg gcgggagtcc
     1381 cttctggggc gcaccgtgaa agaggtcttg ccgggcacgg agcgccactg gatcgacacc
     1441 tacggccggg tggcgctgac gggggaatcc gtcaccttcg aaaacatctc ggcggaactg
     1501 gggcgctact tcgaagtcac cgcctattcc cctcggcggg gcttttttgc ctgcatcttc
     1561 gccgacgtca ccgcccgtcg ccgggcggaa gaagccctgc ggatcagcga agagcgcctg
     1621 cgcttcgccc tggaagccac cagcgacggc ctgtgggatg cggacttttc cacgggggag
     1681 gtctactgga gtccccgggc ctatgccatc ctggggtaca ccccggagga gggccccatg
     1741 accctgaacc ggtgggagga gctcctgcac ccccaggatc ggaaggccgc agaagagctt
     1801 gtccaaagcg ccttctccgg ggagagcgag gggtataccc tagagtatcg cctggccgcc
     1861 cggaacggag attggcgctg ggtgatggac cggggcaagg tcgtcgagcg ggacccctcc
     1921 gggaagcccc gccgcatcgt gggcgccatg gcggacatca cggagagaaa ggccctggaa
     1981 gaggaacgac gggtgctgga aaaatacctc cagaacgtgg tggactccat gccctccatc
     2041 ctggtggggg tggacgtcca gggaagggtg acccagtgga acgccgaggc ggaacgccgc
     2101 acgggccttt cacggcgcca cgccctggga cgtccggtgc gggagtgcgg caattttccg
     2161 gaggagacca tggccctcct ccaggaggcc atcgccctcc ggtccctcca aaaggccgag
     2221 cgcatctccc tggaagaggg gggggagcgg cgttggtcgg atgtcttggt ctatcccctg
     2281 gtggccaacg gcgtcgccgg ggcggtggtg cgggtggacg acgtcaccga gcggacccgg
     2341 atggaggaag taatggtcca gaacgagaag atgatgtctt taggagggct ggcggcgggc
     2401 atggcccacg agatcaataa ccccttggcg ggaatcctcc agggggctca aaacatccag
     2461 cgtcgcctct cccccgagat gcgaaagaac gtcgaggtgg cagaagagtg cggcgtctct
     2521 ctggaggccc tgcggcgtta cctggaagcc cgggatatct tcgccttttt gaaagatatc
     2581 cgcgaggctg gggaacgagc ggcggacatc gtgcgtcaca tgctcacctt cagccgccgg
     2641 gggagcatga cgaaggctcc ttgtgctctg aaagacctgc tagaccatac gctgaagatc
     2701 gcccgggccg atttcgacct cagggtgcgt tacgacttcc agaaaatcct tctggagcgg
     2761 gaagatgacc tccacctccc ctccgtgtcc tgcgtggcgg cggagataga gcaggtgatg
     2821 ctgaacctgc tgcgcaacgc cgcccaggcc ctgagcgctt ccccctccac ggcctttcct
     2881 cgcatccggg tggtgacccg gcgggaggga gactgggccg tgatcgaggt gtgggacaac
     2941 ggccccggag tgcccgaaga gaaaaaacgt cacatcttcg agcccttctt caccaccaag
     3001 aaagcgggtg agggcacggg attggggctt tccgtgtcgt atttcatcgt ggtgagccgt
     3061 cacaacggct ggatgaaggt ggatgatgct cccggagggg gagcggtctt tcaggtcttt
     3121 tttcccctgg aaagggggga ttagcgtggc cgaaccctgg cgcgtgttgg tggtggaaga
     3181 tgacggaact gtgggagaca ccttggcggc ctttctggag gacgaaggct tcgacgtgcg
     3241 cttggtccgc agcggagaag aggccctggc gatcctgaga agcgggtgga tcccccgggt
     3301 gggggtcgtg gatctgcgcc tccccggcat ggacggaagg gcggtggtgg agtgcgcccg
     3361 ggtgctggcc ccgtcgatgc attttctggt gcacacggga tcccccgggg gcgtggtgga
     3421 agacgtggcc tcgtccatgg gagtcgacgc aaaagatatt ttcatcaaac ccgtgcggga
     3481 cttgggactc ctggtggaac gactgcgccg cagcggcgaa ggggtagatg agcatggggc
     3541 atgaccccgg aggcccaaag cacattctgg tgatagaaga cgacggcgcg gtgcgacggc
     3601 acctggcggc gtacttggag gattgcggct atcgggtgac ggcggcggcg gagggggagg
     3661 gggggctgga gatggcccgg acccagcacc ccgacttggt catcaccgac ctgcacatgc
     3721 ccgggatctc gggctttcag gtcctctcgg ccctggcgga agagtttccc gccctccctg
     3781 tgttggtggt ctccggcacg gacctcctga cggaagtggt ggaggccctg cggcgagggg
     3841 cctgggacta cctgatcaag cctctgggag agatgggggt gttggagcat gccgtggagc
     3901 gagccctgga gcgcgcctgg ctcctggaag agaaccgacg ttcccgggag gagctggaag
     3961 aggccgtggc tcggaggacc caggagctcc agaccctcct cacggaaagg acggtcctcc
     4021 tgcgggaggt acaccaccgg gtgaaaaaca actttcaggt aatatcgagc ctcctccaga
     4081 tccgggcccg aagctcaaga catccggagg tgcggaactt ttcctccgac tttgcccagc
     4141 gcatccggtc catggccctg gtgcacgacg ccctgtacca gaccctggac ttcgcccggt
     4201 ggaacttcgg ggagtacctc aagcacctgg tgagggacct ggctcggggc gacggctccc
     4261 cgggcccgga ggtgcgggtg gaagccgccc ccttggaggt gagcctcgac ctggcggtgc
     4321 cctgcgccct catggcccac gaactggtgt ccaacgccct gcgccacgcc tttcctgagg
     4381 gcggggggca ggtgatcgtc tccctgagcg ccggggcccc ggggatgggg cacctggagg
     4441 tgcgagacga cggcgtgggc ctttcccagg agaccctgga ccaggccgaa gggggcatgg
     4501 ggctccagat cgcccgcatc ctggtgcagc agctctcggg caccctgg
//
LOCUS       DAOOER010000104         4509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_18174_length_4509_cov_7.204311, whole genome shotgun sequence.
ACCESSION   DAOOER010000104 DAOOER010000000
VERSION     DAOOER010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18174_length_4509_cov_7.204311"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..1961)
                     /locus_tag="PL162_09100"
     CDS             complement(240..1961)
                     /locus_tag="PL162_09100"
                     /inference="COORDINATES: protein motif:HMM:NF017400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HQF92213.1"
                     /translation="MKGEAKTVVRGLEVTSFPVEIVDVLRREGLPRHLVLVRVSGALM
                     DRTGGIAAGMSGSPVYVQNKLVGALGYGWEFSDHRLGLVTPAEDLAEVWNWPDRLPSF
                     RPPVPLSGERSEEGQKGGGPGGALAECFPLTGGSFAFGGVPLTLGERSAFGPEAPLQT
                     PLLADGMSPRAREHLGSSLGVRVLAGGGTPSDLFIDGRATLRPGDSVGVLLAWGDVTV
                     GSTGTLSALDQQGRFVAFAHPMLNRGGVAYPLARSVVHGVVPSVEVPFKLGSPKSLIG
                     IVTQDRPQGVGGYLGRFAPAVDFSVTARDSDTKREIKKRFHVVDDPFLLEEIVPEAIL
                     GLLDDAWARVGPGTIFSTLRIEGGGLSQEWKRENIFFSTKDPVAEVIKECRDLVKAFA
                     INEFHEIRPLGVHLDVEFSLDPRVLFVEDVILSPQDAAPGQKVQVSVTLRPYRREPFE
                     KVFTLTVPKDAEGTCDVLVRGGGIAESDQESLLQGWRSIKDLPSLLAELSAKESNNEL
                     VVELVSEGNKEEGSDKGTPGEEDQMLLSELQEKRRREGTLRTFRSNYYIEGLLRRPLR
                     VVTPKGP"
     gene            complement(2141..3245)
                     /gene="prfB"
                     /locus_tag="PL162_09105"
     CDS             complement(join(2141..3172,3174..3245))
                     /gene="prfB"
                     /locus_tag="PL162_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_143330735.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HQF92214.1"
                     /translation="MAIVPTTTILEELRSLSGELRDSLDPETLAKDTEALQAQTVRED
                     FWGRDDAQEVLRKLARLQERAQQWIEVNSEIEELVLLIDLLGEAADEDLEGEFYRRAA
                     ALKKTMEKKQLTLLLNDEYDENDAILTIHPGAGGMDSADWADMLHRMYLRWAEIEGFH
                     TTILDLSPDIEAGIKSATVLVKGAFAYGYLKAERGVHRLVRISPFDAAKRRHTSFASL
                     DVAPEMPEDAGVEIRSEDLKMDTFRASGAGGQYVNMTDSAVRITHIPSGIIVSCQVER
                     SQHMNRATAMRILRARLFERTLRERQEKLEALGGEKKEIGWGSQIRSYVLHPYTLVKD
                     HRTNYEVGNVQAVLGGELQDFIMAYLRWKKKNQ"
     gene            3366..4364
                     /locus_tag="PL162_09110"
     CDS             3366..4364
                     /locus_tag="PL162_09110"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HQF92215.1"
                     /translation="MSQKKETRQILGSLLWEAQGGVVSGPSLCARAGITRQAVWKTME
                     RLLEEGFPLEIIPRRGYRLCQEAPDTLHPLWLSLAASAVPGKPTFEVLDEVPSTQYVA
                     REIFRRGVPSPVVVLAESQSEGRGRRGRAWSSPRGCGIYMSALLRPSLSPRQAPLLSL
                     AAGLAARRGIWRKAGIACDLKWPNDLLRGNGKVCGILTEVASDPDQIHFAVVGMGINV
                     NLCGGLVPKDPSPTPPAFLQREGDPLIHRGWLVVAILEELVSLVSLLESGGSQGLVAE
                     YESHCATLGRAVRVITDQEIILGTARGISPEGALVVECTEGVRQFVSADVIHASLG"
BASE COUNT          872 a         1406 c         1334 g          897 t
ORIGIN      
        1 cgccgatccg gtccatgtgg tcgaaaaagt catgccattc ccatcgccct cggaaaatgc
       61 cccgggccat ggaacggccg taaagggcga acgatcccag agacgcgaaa aatgcgagga
      121 cctgtcggcc gatccaggcc atcagcctca cggaccccac tcctttccga cgaaagggct
      181 ccgacgacgt atcctcccct tcggtgtcaa acctccgggg tcttctttgc ccctcggtat
      241 cacggcccct taggagtgac taccctcagc gggcggcgta agaggccttc gatgtagtag
      301 tttgatcgaa aggtacgcag ggttccctcc cggcggcgtt tttcctgaag ctcgctcagg
      361 agcatctgat cctcttcccc gggagttccc ttgtccgatc cctcttcctt gtttccttca
      421 ctgaccaact ctaccacaag ctcgttgttt gactctttgg cagaaagctc ggccaggagc
      481 gacggaaggt ccttgatgga tcgccaccct tgcagcagcg attcctgatc cgattcggcg
      541 atgcctcctc ctcgcacgag gacgtcacag gtcccttcgg cgtctttagg tacggtgagc
      601 gtgaaaactt tttcgaaggg ttcccgccga taggggcgaa gggtgacgga gacctgcacc
      661 ttctgacccg gagccgcatc ctggggcgat aggatcacat cctccacgaa gagcacccgg
      721 ggatccaggg agaactccac atcaagatgg actcccaggg ggcggatttc atgaaattca
      781 ttgatggcaa aggctttgac gagatctcgg cattctttga tgacctccgc cacggggtct
      841 ttcgtagaga agaaaatgtt ctcccgcttc cattcctgcg aaagcccccc tccctcgatg
      901 cgaagcgtgc tgaaaatcgt tcctggtccc acgcgcgccc aggcgtcgtc caagagaccc
      961 aagatcgctt cggggacgat ctcttccagc aggaaggggt cgtccacaac gtgaaacctc
     1021 ttttttattt ctcgcttagt gtcgctgtcc cgggccgtca cggaaaaatc caccgccggg
     1081 gcgaagcgac cgaggtaccc tcccacaccc tgggggcggt cctgggtgac gatgccgatg
     1141 aggcttttgg gagagcctaa cttgaacggc acttccacgc tggggaccac gccgtgaacc
     1201 accgaccggg ccagggggta ggccacacca ccccggttta acatggggtg ggcaaaggcc
     1261 acgaagcgcc cctgttggtc cagggcagaa agggtgcccg tagaccccac ggtgacgtct
     1321 ccccaggcca gaaggactcc caccgaatct ccggggcgta gcgtcgccct cccgtcgatg
     1381 aaaaggtccg agggggtacc tcctcccgcc aggacgcgga cccccaggga agaccccaga
     1441 tgctcccggg cccgagggct catcccgtcc gccaggaggg gggtctggag gggcgcctcc
     1501 gggccaaaag cactccgttc gcccaaggtg agggggacac ccccaaaggc aaagctccct
     1561 ccggtcaagg gaaaacattc cgccagtgcc ccaccgggcc cccccccttt ttgaccctct
     1621 tcggaacgct ccccggagag gggaacgggg ggacgaaacg aagggaggcg atcgggccag
     1681 ttccacacct ccgccagatc ttccgcagga gtcaccagcc ccaggcgatg atcactgaac
     1741 tcccatccgt accccagagc tcccaccagc ttgttctgga catagaccgg ggatccgctc
     1801 atgcccgccg cgatgccacc ggtacgatcc atcagcgctc cggagacccg gaccaacact
     1861 aagtggcgag gaaggccttc tcggcgcaac acgtccacga tttccacggg gaacgaggtt
     1921 acttccaacc cccgtaccac ggtcttcgcc tcccccttca tccccgaccg gagctgagac
     1981 acgggaaaca caggatctcg ggggacaaag ggccccgaag cttctgcacc cccaggattt
     2041 ccattccaga aaaaaagcac ccacgcccct aggagggccc ctaaaagccc cttgaagggg
     2101 cgacgcgata cgctcgaagg cgagcttaat tcaatgagtt tcattgattt ttctttttcc
     2161 atcgcagata ggccatgata aaatcctgca gttcgccccc taagacggcc tggacgttgc
     2221 ccacctcgta attggtgcga tggtccttga ccagcgtgta ggggtggagg acgtaggaac
     2281 ggatctggct cccccatccg atctccttct tctctccccc cagggcttcg agcttttcct
     2341 ggcgctcccg cagggtgcgc tcaaagaggc gagccctcag gatgcgcatg gccgtggcgc
     2401 gattcatgtg ttgagatcgc tccacttgac agctgacgat gatccccgaa gggatatggg
     2461 tgatccgcac ggcggagtcg gtcatgttga cgtactgtcc ccctgcgccg ctggccctaa
     2521 aggtatccat tttaaggtct tcgctacgaa tctccacccc cgcgtcttca ggcatttccg
     2581 gggccacgtc cagggatgcg aagctcgtgt ggcgcctctt ggcggcgtcg aagggagata
     2641 tgcggaccag gcgatggact ccccgctccg ccttgagata gccgtaggcg aaggctccct
     2701 tcaccaacac ggtggcgctt tttattcccg cttcgatgtc gggagagagg tctaaaatgg
     2761 tggtatgaaa cccttcgatt tcggcccacc gaaggtacat gcggtgcaac atgtccgccc
     2821 aatccgccga atccatgccc ccggctccgg gatggatggt gaggatcgcg tcgttctcgt
     2881 cgtactcatc gttcaacagc aacgtcagct gctttttttc catggtcttt ttcaaggcgg
     2941 cagctcgacg gtaaaattcc ccctccagat cctcatccgc cgcttctccg aggaggtcga
     3001 tcagcaaaac caattcttct atctcagagt ttacttcgat ccactgctgg gcgcgctcct
     3061 gaaggcgagc cagctttcgc aaaacctcct gggcgtcgtc tcggccccag aaatcctctc
     3121 tgacggtttg ggcctgcagg gcctccgtgt cctttgccaa ggtttcgggg tcaaaggctg
     3181 tcccgcagct ccccagaaag ggagcgcagt tcctccaaaa tcgtggtggt gggtacgatg
     3241 gccatacgca aaaacctcct tactggaatg gcgacggggc gggaatcaaa attcctcgtt
     3301 gcgaccgccc gggctctatt atatcatcgc ctgaggctcc gtgcggggtg aagggggaga
     3361 gtgccatgtc ccagaagaaa gaaacgcgcc aaatcctggg atctctgctc tgggaggctc
     3421 aggggggcgt ggtatccggg ccatccctct gcgcccgggc gggcatcact cgccaggcgg
     3481 tatggaaaac tatggaaagg cttctagaag agggtttccc tctggagatt atccctcggc
     3541 gggggtaccg tctatgccag gaggctcccg acaccctcca ccctctttgg ctttcgctgg
     3601 cggcatcggc ggtgccgggg aagcccacct tcgaggtttt agacgaggta ccctctacgc
     3661 agtatgttgc ccgggagatc tttcgccggg gagtcccttc ccctgtcgta gttttggccg
     3721 agagccaatc cgagggccgg ggacggcgcg ggagggcatg gagttctccc cgggggtgcg
     3781 gtatctacat gtccgccttg ctcaggccgt ccctttcccc tcgccaggct cccctgttga
     3841 gtttggccgc cgggttggcg gcgcgtcgag gaatttggcg gaaggcgggg atcgcctgcg
     3901 accttaagtg gcccaacgat ctgctccgag gaaacgggaa ggtgtgcggc atcctcacgg
     3961 aggtcgccag cgaccccgac caaatccatt ttgccgtggt gggcatgggg ataaacgtca
     4021 acctctgcgg ggggttggtc ccaaaagacc cctcccccac tcctccggcc ttcctgcagc
     4081 gagagggcga ccccctgata caccgggggt ggttggtcgt ggccatcctc gaagagcttg
     4141 tgtccttggt ttctttgtta gaatctgggg gaagccaagg cctggtagcg gaatacgaga
     4201 gccactgtgc caccctgggg agagccgtcc gggtgatcac agaccaggag atcatcctgg
     4261 gcacggcgcg agggatttct cccgaggggg ctttggtggt agagtgcacc gaaggggtgc
     4321 ggcagtttgt ctcggcagac gtgatccatg cctccttggg atgacgtcac gcccccccag
     4381 gtggggcttg aggagcctta ggatattctt cgaacaatat gagtaatgga taacaagggg
     4441 gtgactggca tggcgaagct catggaccgc tctcgtacca gtgggtgagc ggcaaagata
     4501 ggtccggcg
//
LOCUS       DAOOER010000105         4496 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_18249_length_4496_cov_7.061698, whole genome shotgun sequence.
ACCESSION   DAOOER010000105 DAOOER010000000
VERSION     DAOOER010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 4496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4496
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18249_length_4496_cov_7.061698"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PL162_09115"
     CDS             complement(<1..518)
                     /locus_tag="PL162_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92216.1"
                     /translation="METPTFAAWVRSQGRAVHRGGGMDWYIYAGALVPLTLFPVFPRL
                     SPSEERKLLKESGALALRYTPPVPPGTETPFWYVVCPHYAFDDLSAKMRNQVRRGRSR
                     CAVERLAPETLAEEGWACLRAARAHYGEGAPEEETFRREIRAQGEPPFEFWGVRVGGV
                     LGAYAICVVEGDR"
     gene            complement(528..>1207)
                     /locus_tag="PL162_09120"
     CDS             complement(528..>1207)
                     /locus_tag="PL162_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92217.1"
                     /translation="MFLRDYAVRRFVEGARRLNRLKTPMDVVLETAELYGFPCHHMAA
                     LDVFARMGLINTRDFEPRCDYLECWEIDPKIIPWLRRFVPRATVREGDSIAATRERRH
                     ARSHYDLIVLDNPPCLFGDGYCEHFDHFPDILDYGAPRCVVAFDIIPDLRNANSGSPG
                     EPYFEQWRQRRREFYPGSDGVTVPVDHAREVYRTMMISRGFRVVGETLVPRNRMVTFL
                     GYFLERP"
     assembly_gap    1208..1307
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1351..2868)
                     /locus_tag="PL162_09125"
     CDS             complement(1351..2868)
                     /locus_tag="PL162_09125"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HQF92218.1"
                     /translation="MRLEIDRESPTPIYVQIRNRLRERILSGELPEGLRLPPERELAQ
                     HLGVNRTTVLNAYRELKAEGLVSAHVGRGTVVCRMEKPQDEGMETLSAPLHWERYLRP
                     LPDDSRDGIMRQIWDRCPSPEVACFSCGMPDRGLFPTELMGELHTRAFREAGPEVLDL
                     MPVEGYFPLREALADLMRHRGINASPEDVVVTGGSQQGVDLLARMMVESGDGVAAEDP
                     LYVNGRAVLNSLGARIYGVPMDCDGMRPDVLEGLLRRYALKFVYVGPNFQNPTGVVMS
                     SPRRCEILDLARRHQVPVLEDDPYFDLRYEGADLPPIKALDSSGHVLYASTASKTLVP
                     GLRLGWVVLPRPMVRRFVRFKRGADLATNGVAQWVLHRYIVEGHYQRHLERVRREYAH
                     RLKAMEDLLAQEEPRTGWRWHHPQGGMFLWLELPEGVDAQRFALRAAARGVVCAPGSI
                     YAVDGGNERFLRINFTLPSQEAMAQGVPLMVKALHECLLEDRTLRRSDAESLSAV"
     gene            3155..4000
                     /locus_tag="PL162_09130"
     CDS             3155..4000
                     /locus_tag="PL162_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92219.1"
                     /translation="MRPKDLGPLSRGRPGGDLREKGQTSDGAPPSRGRFLTGPGGARG
                     VLALVAAVALGIAFPLDPILPGVAGALPRSGAVPGYEHIRFDDLTLDPDRQELRLVLE
                     NTTPQDLYFFATLFLSDREGGTVFRPRIREFLKKEGRTPCRLPLPGKKDPQVCQRLTD
                     LRWEVTRKNRALVEVRPVVSVVRGECGQGFSWEGLRFFGSDYGYEIRGKIVCPPGKAS
                     WSRGVFRLRLEDVAHGTILEKDVGVKDLSPGVKRDFVLRGREPTLRELRSLTTHRISL
                     VKVFP"
     gene            complement(4124..>4496)
                     /locus_tag="PL162_09135"
     CDS             complement(4124..>4496)
                     /locus_tag="PL162_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92220.1"
                     /translation="FHQADGVGLFVCHDITSFFIFKPRPRGTFPGGLLVRGERKESGT
                     MSRAFTKDDDDAALMRERDDELRRLREWLAIQEKKRRFLEEDPKGQAIDEAQRVAWLE
                     SVRADIAKTLKQLEDLEKSEE"
BASE COUNT          795 a         1477 c         1393 g          731 t
ORIGIN      
        1 cgatctcctt ccaccacgca aatggcgtaa gcccccaaaa cccctcctac ccgcacgccc
       61 cagaactcga aggggggttc cccctgggct cggatttccc tccggaaggt ctcttcctcc
      121 ggggcccctt cgccgtagtg ggcccgggcg gcgcgcaggc aggcccaccc ctcctccgcc
      181 agggtctcgg gggccaggcg ctccacggcg caccgagacc gcccccggcg cacctggttg
      241 cgcatcttgg ccgagaggtc gtcaaaggcg taatggggac agaccacata ccagaagggg
      301 gtctccgtcc ccggaggcac ggggggggta taacgcaacg ccagggcgcc gctttctttc
      361 agaagctttc gctcctccga agggctcagg cgggggaaga cgggaaagag cgtcagcggt
      421 accagggccc cggcgtagat gtaccaatcc attcctccgc cccgatggac cgcccgcccc
      481 tgggaccgga cccaggcggc gaaggtgggc gtttccacgg cggcgcctca ggggcgctcc
      541 aggaagtatc ccaggaaggt gaccatgcga ttgcggggga ccagggtctc ccccaccacc
      601 cgaaagcccc gggagatcat catggtccga taaacctccc gggcgtggtc cacggggacc
      661 gtcacgccgt cgcttcccgg atagaactcc cgtcgccgct ggcgccactg ctcgaaatag
      721 ggctccccgg gcgatccgga gttggcgttt ctcaggtccg ggatgatgtc aaaggccacg
      781 acgcaccggg gcgccccgta gtccaggatg tccgggaagt ggtcgaagtg ctcacaatat
      841 ccgtcgccaa acaggcaagg ggggttatcc aagacgatga ggtcgtagtg cgaccgggcg
      901 tggcgtcgtt cccgggtggc ggcgatggag tccccctccc gcacggtggc ccgggggacg
      961 aagcgccgga gccaggggat aatcttgggg tcgatctccc agcactccag gtagtcgcag
     1021 cggggctcga aatcccgagt gttgatgagc cccatgcggg cgaagacgtc cagggcggcc
     1081 atgtgatggc agggaaagcc gtagagctcc gccgtctcca ggaccacgtc catgggggtc
     1141 ttcaggcgat tcaggcgccg agccccctcc acgaaacggc gcacggcata atccctcaaa
     1201 aacatcannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnccg gaagggggca
     1321 ggcctaaaag gacctcgaag gaaaggggcg tcacaccgca gaaagggatt ccgcatcgga
     1381 ccgccggagc gtccgatcct ccaggaggca ctcgtgaagg gccttgacca tgagggggac
     1441 cccctgggcc atggcctcct ggctcggcag ggtaaaatta atcctcaaaa atcgttcgtt
     1501 acccccatcc accgcataga tggatccggg ggcacagacc accccccggg ccgccgctcg
     1561 cagggcgaag cgctgggcat ccactccttc ggggagctcc agccagagga acattccccc
     1621 ctggggatga tgccaccgcc accccgtccg gggctcctcc tgggccagga gatcttccat
     1681 ggccttgaga cgatgggcgt actcccggcg caccctttcc aggtggcgct ggtagtgccc
     1741 ttccacgatg taacggtgca acacccattg ggcgacgccg ttggtggcca aatcggctcc
     1801 ccttttgaac ctcacgaagc gcctcaccat ggggcggggc aagaccaccc atccgaggcg
     1861 caggcccggc accagggtct tcgaggccgt gctggcgtag agcacatggc cggaggagtc
     1921 cagggccttg atcggaggga gatcggcccc ttcgtagcgg aggtcgaagt aggggtcgtc
     1981 ctccaacacg ggcacctgat ggcgtcgggc caggtccagg atctcgcatc tccgggggga
     2041 ggacatgacg acccccgtgg ggttctggaa attgggcccc acatagacaa actttagggc
     2101 gtagcgccgc aggagcccct ccaagacgtc cggcctcatg ccatcgcagt ccatgggaac
     2161 gccgtagatc cgggccccca gggagttgag gacggcccgg ccgttgacgt aaagggggtc
     2221 ttccgccgcc actccgtccc cggattccac catcatacgg gccaagagat ccactccctg
     2281 ctgcgacccc cccgttacca ccacatcctc cggcgaagcg ttgatgccgc gatggcgcat
     2341 gaggtccgcc agggcctctc gcagggggaa atacccctcc acgggcatga ggtccaacac
     2401 ctcaggtccc gcctctcgaa aggctcgggt gtggagctcc cccatgagct ctgtggggaa
     2461 aagccccctg tcgggcatgc cgcaggagaa gcaggccacc tcgggggaag gacatcgatc
     2521 ccagatctgg cgcatgatcc cgtcccggga gtcgtcgggc agggggcgaa ggtagcgctc
     2581 ccagtgcaag ggggccgaca gagtttccat cccttcgtcc tggggctttt ccatgcgaca
     2641 caccacggtg ccccgcccca cgtgggccga aaccagcccc tcggccttga gttctcggta
     2701 ggcattgagc acggtggtgc gattcacacc caagtgctgg gccaactcgc gctccggcgg
     2761 aagccgcaac ccctcgggga gctcccccga gaggatgcgc tccctgaggc ggttgcggat
     2821 ctgcacgtag atgggcgtgg ggctctctcg gtcgatctcc agtcgcaaaa taaaactctc
     2881 ccttctgtcc gccgcccgtc agccggaacc cggatcttca tccgaggccc ggcgactcct
     2941 cgtcttcccg gggcgtctcc caaagaatct ccccttcgca cggtggggcg cgaaaaatgg
     3001 cccaccaatt ggaccatgcc aaatttacac ctccggggaa ggccttggca agcccccaga
     3061 ttcctttgac gtggtacaat gaccccctaa ggcccccagg tggaggaaag gacctcgggg
     3121 aggcgaagac ttcttcttcc aggaggagat gggcatgaga ccaaaggacc tcggccccct
     3181 ttcgcggggg cgtcccgggg gggatctcag ggaaaagggg cagacctccg atggagcccc
     3241 tccatcccgg gggcgtttct tgacggggcc cgggggagct cgaggagtcc tcgccctcgt
     3301 cgccgccgtc gcccttggca tcgcctttcc cctcgacccc atcctccccg gagtagcagg
     3361 ggcccttccc cgttcggggg ccgtgccggg gtacgagcac atacgcttcg acgacctcac
     3421 cctcgacccc gaccgtcagg agttgcgcct cgtcctcgag aacaccacgc cccaggacct
     3481 ctacttcttc gccacgctct ttctctccga tcgcgaaggg ggcacggtct tccgcccccg
     3541 catccgagag tttcttaaaa aggagggacg cactccgtgc cgccttcccc ttcccgggaa
     3601 aaaagaccct caggtttgcc agaggctgac ggatctccga tgggaggtga ccaggaaaaa
     3661 ccgcgccctg gtggaggtgc gccccgtggt gtcggtggtc cgaggggagt gcggccaggg
     3721 attttcttgg gaggggctgc gctttttcgg cagcgactat ggctacgaaa tccggggaaa
     3781 gatcgtatgc cccccgggaa aagcctcatg gtcccggggg gtttttcggc taagactgga
     3841 agacgtcgcc cacgggacga tcctggaaaa agacgtgggg gtgaaagatc tttcccccgg
     3901 cgtcaagaga gacttcgtct taagggggcg cgaacccacc ctgcgggagc tgcgatccct
     3961 cacgacccac cggatctccc tcgtcaaagt cttcccgtga tccggaggat ccgcgtcgca
     4021 cgagccctcc tgtcgggctc gggggacgcg aacgacgacg aagcccctgc agggacggcg
     4081 gcgcgaaagg accgagaggc ccctccgcaa gccctcggcg ccgtcattct tcgcttttct
     4141 ctagatcttc cagttgcttc aatgttttgg ctatgtcggc ccggacgctt tccagccagg
     4201 cgacgcgctg ggcctcgtcg atggcctggc ccttggggtc ttcttccagg aagcgcctct
     4261 tcttttcctg gatcgccagc cactccctca ggcgtcggag ctcgtcgtcc cgctccctca
     4321 tgagggccgc atcgtcatca tctttcgtga atgcccgact catggtgccg ctctcctttc
     4381 gctccccccg taccaggagg cccccgggga acgttccccg ggggcggggt ttgaagatga
     4441 agaaagaggt gatgtcatgg cagacaaaaa gaccaacgcc gtccgcctgg tggaag
//
LOCUS       DAOOER010000106         4463 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_18413_length_4463_cov_9.974592, whole genome shotgun sequence.
ACCESSION   DAOOER010000106 DAOOER010000000
VERSION     DAOOER010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4463
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18413_length_4463_cov_9.974592"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            547..1833
                     /locus_tag="PL162_09140"
     CDS             547..1833
                     /locus_tag="PL162_09140"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HQF92221.1"
                     /translation="MSFSRLTSLGRAILGSLRPDARAMEFLRYLGPGILVTVGFIDPG
                     NWASNVAAGSEYGYSLLWMVTLSTVMLIFLQHNAAHLGIATGLCLSEAARTHLPAPVA
                     LPVLLSAVGAAVATALAEILGGGIALTLLFGIPPRLGCVGVTILVAWMIVTNSYRRLE
                     RWIVGFVSLIGFAFVFELSQVEIPWGQALEGWVVPRIPSGSLPIVMSVLGAVVMPHNL
                     FLHSEVIQSRQWNRESEEVIQRQLRYEFLDTLLSMGIGWAINSAMILVAAAAFFSAGH
                     TVTALEEASALLQPVLGPAAAGVFALALLFAGLSSSLTAGMAGGSIFAGIFGEPFDMG
                     DRHSRIGVALTLGGALLAIFFITDPFQGLVISQMLLSIQLPLTILTQIFLTASPRVMG
                     PHANSLEENIVLGIIAALVILLNGMLFLSFFGLFPG"
     gene            complement(1977..2543)
                     /gene="msrA"
                     /locus_tag="PL162_09145"
     CDS             complement(1977..2543)
                     /gene="msrA"
                     /locus_tag="PL162_09145"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HQF92222.1"
                     /translation="MEDTKRTQELQTAYFAGGCFWCMVSPFDVLEGIEEVRSGYMGGS
                     VANPTYEDVKGQTSGHYEVIRVRYDPGVITYGKLLQAFWRQVDPTDPDGQFHDRGPSY
                     RTAIFYTTEEQRREALASRSELDASGRFSAPVVTPVLPAPAFYDAEEYHQDFYRKNPG
                     EYQRDRTFSGRDEFIRKHWGEEYFDIYR"
     gene            complement(2830..4059)
                     /locus_tag="PL162_09150"
     CDS             complement(2830..4059)
                     /locus_tag="PL162_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HQF92223.1"
                     /translation="MRQKLREQVKALQGEMVDTLCELVRRPAVSPHEGGQGEEEKVAY
                     LEDLVRRLGYSAVERYDAPDSGAPRGIRPNLVVRVPGKNPGRIWFIAHTDVVPEGDRS
                     LWHSDPFEPVVRDGRVYGRGSNDNGEELVASLYALEALRRLNLVPEREVCLAFVADEE
                     MGSTFGIQYLIGQKLFSPDDLLVVPDGGNEEGNFIQVAEKSILWMEFTVEGVQVHASR
                     PSSGNNACRAANAFSVALDRALHEAFPETNDLFNPAESTFEPTRRAQNVANINTVPGK
                     EVFCFDCRVLPGVPLEEVLKVVDAVQKEHEALSGVSISRKYPQKNQAAAPSAKDAPVV
                     RLLEGAVREVLAVEPTVGGVGGGTCAAFFRAAGIPAVVWGQEADVAHQPDEYTLVEHM
                     VNEAQVFALMMGGSALE"
     gene            complement(4134..>4463)
                     /locus_tag="PL162_09155"
     CDS             complement(4134..>4463)
                     /locus_tag="PL162_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HQF92224.1"
                     /translation="LIDPLADKILVTAALISLVELQRLPAWIVVVIVAREFLVTGLRM
                     VAAAEGVVVAASRGGKAKTVSQIVAISMVMLGIPGGLAAMGVALVLTVWSGMDYLVGC
                     RDIFMKD"
BASE COUNT          716 a         1566 c         1361 g          820 t
ORIGIN      
        1 gggagccgac gccatcgtgg gatttcgcct ttccaccacc tccatcgccc agggggcggc
       61 ggagatcatc ggctacggca cggcggtgcg atatcgccaa caagcatccg taggataaaa
      121 gcgcacgtcg caggggcaaa ggcgtttcgt cccgacgaaa ccctcctgcg acgacagggt
      181 gcggcgtagg ggcgagcgtg ttttccgccc ctacaaaatt tcattacaat aacagggcat
      241 ggccgtggaa tgtgaacgat gatgccggat ggaatctgcg cgctcccggc cgatgcctac
      301 cgaaggtgat gggtgggtgc cgtagggggc gcaaaggcgt ttcaccccga caggaccttc
      361 ctgcgatgac aggggacggc cgtgggggcg agcgtgtttt ccgccccaac agaatttcgt
      421 tgcgataacg tgttgtggtc gcgggggaag cccaggtttc ccgtcccgac aaaaccccat
      481 tgcgataaca gggtatgatc ccggggcgca agcccctgca gcactttgag aaaggagcga
      541 tttcttgtgt ctttttcccg ccttacttcc ttgggacggg cgatcctagg aagccttcgc
      601 cccgacgccc gggccatgga gttcctccgg tacctgggac ccggcatcct ggtcaccgtg
      661 gggttcatcg atccgggaaa ctgggcctcc aacgtggcgg cgggctccga gtacggctac
      721 tccctgctct ggatggtcac cctctccacc gtgatgctca tctttctgca acacaatgcg
      781 gcccatctgg gcatcgccac ggggctgtgc ctttcggagg ccgcccggac ccacctcccc
      841 gctcccgtgg cgctgccggt gctcctgtcg gcggtggggg ccgctgtggc caccgccctg
      901 gcggagatcc tcggaggggg catcgccttg accctgctct tcggcatccc cccgcgcctg
      961 ggctgcgtcg gagtgacaat cctcgtggcc tggatgatcg tcaccaattc ctaccgccgc
     1021 ctggagcggt ggatcgtggg attcgtctca ctcatcggct tcgccttcgt cttcgagctc
     1081 tcccaggtgg agatcccctg gggccaagcc ctggaagggt gggtcgtgcc ccgcatcccc
     1141 tccggatccc tgcccatcgt catgagcgtc ctgggagcgg tggtcatgcc ccacaacctc
     1201 ttcctccact ccgaggtgat ccagagccgc cagtggaacc gggagtccga ggaggtgatc
     1261 cagcgccagc tccggtacga gtttctggat accctgcttt ccatgggcat cgggtgggcc
     1321 atcaacagcg ccatgatcct cgtggcggca gcggcgttct tttcggcggg gcacaccgtc
     1381 accgccctgg aggaggcctc ggccctcctc cagcccgtac tgggccccgc cgccgccgga
     1441 gtcttcgccc tggcgctgct cttcgcgggg ctctcttcct ccctcacggc gggcatggcg
     1501 ggggggagca tcttcgccgg gatcttcgga gagcccttcg acatgggaga ccgacatagc
     1561 cgcatcgggg tggccctcac cctgggggga gccctcctgg ccatcttttt catcaccgac
     1621 ccctttcagg gcctggtgat ctcccagatg ctcctgagca tccagcttcc cctcaccatc
     1681 ctgacccaga tctttctcac tgcgtccccc cgggtgatgg gtccccacgc caactccctc
     1741 gaggaaaaca tcgtcctggg gatcatcgcc gccctggtga ttctgctgaa cggcatgctc
     1801 tttctctcgt ttttcggcct ctttccgggg taggcctgaa acggggcgat ccgcgccgcc
     1861 ctccgtctta gggtgcgacg ggccagaagg tttcccccgt gcgcccggat cggggccgat
     1921 cggagccaga ccgccggcaa aagccccggg gatcggcggc ggccccggcg gaacggtcac
     1981 cggtagatgt cgaagtactc ctccccccag tgcttcctta tgaactcatc ccttccggaa
     2041 aaggtccggt ccctctgata ttccccgggg tttttccggt aaaagtcctg gtggtactcc
     2101 tcggcgtcgt agaacgcggg agccgggagc accggcgtca ccacgggggc ggaaaagcgc
     2161 cccgaggcgt ccagctcgct ccgggaggcc agggcctctc gccgctgctc ttcggtggtg
     2221 taaaagatcg ccgtgcggta ggaggggccc cggtcgtgga actgaccgtc ggggtccgtg
     2281 gggtccacct ggcgccagaa ggcctgcagg agcttgccgt aggtgatcac cccggggtcg
     2341 taacgcaccc ggatcacctc gtagtgcccc gaggtctgtc ccttcacgtc ctcgtaggtg
     2401 gggtttgcca cacttccccc catgtacccc gaacgcacct cttcgatgcc ctccagcaca
     2461 tcaaagggag acaccatgca ccagaagcac ccccccgcaa aataggccgt ctgaagctcc
     2521 tgggttcgtt tcgtatcttc catggtccaa acctccttag gtctccatgt gcgccccccg
     2581 gggggtgttg cccccccctt ccggtggccc cccatggagg ggtgccacaa aactccccga
     2641 tccgccccga accgccgggg cttttatcct ttagcagatc cccgagagga cgggggcccc
     2701 cgggccgacg cgccgcagtc cccccctttt tgagggaccc cccccgagga ctttttcgtc
     2761 acagccatgg ccccgggatc ggctcccggg gccatggctg tgacagaggg gtcgtggcga
     2821 cgaagcgccc tactccaggg ccgagccccc catcatgagg gcgaagacct gagcctcgtt
     2881 caccatgtgc tccaccaggg tgtactcgtc gggttggtgg gccacatcgg cctcttgccc
     2941 ccataccacg gcgggaatcc ccgccgctcg gaaaaaggcc gcgcaggtac ctcctcccac
     3001 gccccccacc gtgggctcca ccgcgagcac ctcgcgcacc gccccttcca ggagacgcac
     3061 cacgggggcg tctttggccg aaggggccgc cgcctggttt ttctggggat attttcgcga
     3121 gatggagacc cccgagaggg cctcgtgctc cttctgcacc gcgtccacca ccttgaggac
     3181 ctcttccagg gggacgccgg ggagcacccg acagtcgaaa cagaagacct ccttgcccgg
     3241 cacggtgttg atgttggcca cgttttgggc tcgacgggtg ggctcgaagg tggactcggc
     3301 ggggttgaag agatcgttcg tctcgggaaa ggcctcgtgc aaggcgcggt ccagcgccac
     3361 ggaaaaggcg ttggcggccc ggcaggcgtt gttcccgctg gaggggcggc tggcgtggac
     3421 ctggacgccc tccacggtga actccatcca caggatggat ttttccgcca cctggatgaa
     3481 attaccctcc tcgttgcccc cgtcggggac caccagcagg tcatcggggg aaaagagctt
     3541 ttggccgata aggtactgga tgccgaaggt gctccccatc tcctcgtccg ccacgaaggc
     3601 caagcagacc tcccgctccg gcaccaggtt cagccgacgg agggcttcca gggcgtaaag
     3661 gctcgccacc aactcctcgc cgttgtcgtt gctaccccgg ccgtagaccc gcccgtctcg
     3721 caccacgggt tcgaaggggt ccgagtgcca cagggagcga tccccctcgg gcaccacgtc
     3781 ggtgtgggcg atgaaccaga tccttccggg atttttgccg ggcacccgca ccaccaagtt
     3841 gggacgaatt ccccggggag ccccggaatc cggggcatcg tagcgctcca ccgccgagta
     3901 tcccaggcgc cgcaccagat cctccaggta cgccaccttc tcttcctctc cttgccctcc
     3961 ctcatggggg gagacggcgg ggcgccgcac gagctcgcag agggtatcca ccatttcgcc
     4021 ttgcagggcc ttcacctgtt cccgcaactt ctgccgcatc gtcccatcct cgctttctcg
     4081 ccccgacccc atgggccagg gccctttccg ggggtagggc ccccggggcc acgctaatct
     4141 ttcatgaaaa tatctcgaca ccccaccagg taatccatgc cggaccagac cgtgagcacc
     4201 agcgccaccc ccatggcggc gagccctccg ggaatgccca gcatcaccat gctgatggcc
     4261 acgatctggc tcaccgtctt ggccttgccc cctcgggagg cggccaccac caccccttcg
     4321 gcggcggcca ccatcctcag ccccgtgacc aaaaactccc gggccacgat gaccaccacg
     4381 atccaggccg gaaggcgctg aagctccacc agggaaatga gcgccgccgt gacgaggatc
     4441 ttgtccgcca aggggtcgat gag
//
LOCUS       DAOOER010000107         4439 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_18547_length_4439_cov_6.492016, whole genome shotgun sequence.
ACCESSION   DAOOER010000107 DAOOER010000000
VERSION     DAOOER010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18547_length_4439_cov_6.492016"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="PL162_09160"
     CDS             <1..890
                     /locus_tag="PL162_09160"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HQF92225.1"
                     /translation="DGRHGGALLPLSVRGTRLTGGTHRLAVASAQMKTALLLSGLSAS
                     EPTTVLEPLATRDHTERLFAFLGIPCTRRDNAVTVFPCGEIPGGRWRIPGDFSAAAFW
                     LVAAALRPDSEVRIARCGLNPTRTGALEVLRRMGLEVRIENEGESGGEPEGDLVVRSA
                     PLSGTKISPEEVPLLVDELPVLAVAASQACGITEIRGAGELRVKECDRIAAVTEGLRA
                     LGGDVEEREDGWIIRGGGPLHGGRVRSFGDHRIAMAFAVAGLATEGPVEIDDPRCVAI
                     SYPRFFETLEALTSGEIPS"
     gene            887..1732
                     /gene="aroE"
                     /locus_tag="PL162_09165"
     CDS             887..1732
                     /gene="aroE"
                     /locus_tag="PL162_09165"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HQF92226.1"
                     /translation="MTGLVALLGDPVSHSLSPRMHHAAFEALGLDLRYLAFRVDAAHF
                     PEALRGLRALGAVGANVTVPHKERAFALVDEVSPEAVQCGAVNAVTFDRGRAVGHNTD
                     GRAIRETLESLGAPEGPALLLGTGGVARAAAGALADRGADPVYIAGRDLEKGKNLVRE
                     LVSRGIRANFEVVPLDALPGNCLLGVHATSLGLPENPFPPTILEAMMGALREGGALLD
                     LVYSPGGTPLVAAARRRGLNARDGTEVLLRQGAASFTLFTGKAAPLRAMARALGIGEI
                     PGVAS"
     gene            1729..2895
                     /gene="aroC"
                     /locus_tag="PL162_09170"
     CDS             1729..2895
                     /gene="aroC"
                     /locus_tag="PL162_09170"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HQF92227.1"
                     /translation="MTWRFLTSGESHGRGLLVVVDGLPAGLELCLEDLASELARRRRG
                     HGRGPRMALERDEIQVWGGLRNGRTTGAPVGVALANAEADQWASAMHPWHTSPEAVEA
                     RRVTAPRPGHADLAGALKYRHSDMRDVLERASARTTAPRTVAGTLARRLLAHCGIAVR
                     GAVLSIGGVSVPPPRDDAGWERARTSPLGCATEEGEAMLAAPIDRARGEGDTLGGTFV
                     VSVRGVPAGLGSGVEWDRRLDARLGAALLSIPGIKGVEIGEAFAAAALTGSAVHDPID
                     GRGGEWRRPSNRAGGIEGGMSNGEEVVIRGAMKPIPTLRKPLPSFDVATGRSGAAHAE
                     RSDVCAVAAACVVGEAMVAWVLGGELVEQFGGDTVEDLRDRLEAHRRRVKGWML"
     gene            2892..>4439
                     /locus_tag="PL162_09175"
     CDS             2892..>4439
                     /locus_tag="PL162_09175"
                     /inference="COORDINATES: protein motif:HMM:NF013887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional shikimate kinase/3-dehydroquinate
                     synthase"
                     /protein_id="HQF92228.1"
                     /translation="MTTPLGSPPLSFNRPLFIGGFMASGKSTVGRLAARLLGVPFLDT
                     DRMIEERARKSVGEIFAQEGEGAFRSQEAAVVREILSLRSVVVALGGGTLEDPGLRDE
                     IAEKGILVTLAVSAETARRRGASGPDRPLLRGDIAALLASRAPGYAAGRFSLDTEHFS
                     PEATAKELVTRLFGDLPPALPPRGPTRLAPFPGGGSEVFIGPGILREIPALVPGGDRA
                     RIVGDGITAPLFAPLMALAGEAYLLPRGEGAKTLYQVQALYDHFARSSVDRTTPVAAL
                     GGGTVGDCAGFAAATWMRGVPLIQCPTTLLAMVDSAIGGKTGVNIPQGKNLVGAFHHP
                     IAVVADAECLQTLPAEEFRQGLAEAVKYAVGEDQGLLEELEKSRGSILARHPAALTHL
                     VERCVRLKLAVVAEDGDETRGIRDRLNLGHTVGHAMEAASDFRWKHGDAVAAGLAVAL
                     TLGGRLGSCSSAFVARGRELLEALGLPWRPDLPWGELLPFLGLDKKFHCGQCRFVLPV
                     EGARCKVR"
BASE COUNT          626 a         1547 c         1611 g          655 t
ORIGIN      
        1 tcgacggacg ccacgggggg gccctcctcc cgctgagcgt ccggggcacc cgcctcaccg
       61 gaggcaccca ccgtctcgcc gtggcgagcg cccagatgaa gacggccctg ctgctctcgg
      121 ggctctccgc cagcgagccc accacggtgc tcgaacccct tgccacgcga gaccacacgg
      181 aacggctctt cgcctttctg ggcattccct gcacccgtcg ggacaacgcc gtgacggtct
      241 ttccctgcgg ggaaattccc ggagggcggt ggcgcattcc cggagacttc tccgccgccg
      301 ccttctggct cgtggccgcc gccctgcgcc ccgattccga ggtgaggatc gcccggtgcg
      361 gcctcaaccc cacccgcacc ggggccctgg aggtgcttcg ccgcatgggg cttgaggtgc
      421 gaatcgaaaa cgagggggaa agcggcggag agcccgaggg agacctggtg gtccgcagtg
      481 cccccctctc cggcacgaag atctcccccg aagaggttcc cctcctggtg gacgagctcc
      541 ccgtgctcgc cgtggcggcg tcccaggcct gcggcatcac ggaaattcgc ggcgcgggag
      601 agctgcgggt aaaggagtgc gaccgcatcg ccgccgtgac ggaggggttg cgcgccctgg
      661 ggggcgacgt ggaggagcgg gaagatggat ggatcatccg gggaggaggg cctttgcacg
      721 gcggccgggt gcgcagcttc ggcgaccacc ggatcgccat ggccttcgcc gtggcgggcc
      781 tcgccaccga aggccccgtg gagatcgacg atccccggtg cgtggccatc tcctatccgc
      841 gctttttcga gaccctggag gccctcacct cgggagaaat tccgtcatga cgggcctagt
      901 cgccctttta ggggatcccg tatcccacag cctttccccc cggatgcacc acgccgcctt
      961 cgaggccctc ggcctggatc ttcgctatct cgccttccgg gtggacgcgg cgcactttcc
     1021 cgaggccctc cgggggctcc gagccctggg ggcggtgggg gccaatgtca ccgtgcccca
     1081 caaggagcgc gccttcgccc tggtggatga ggtctcgccg gaggcggtcc agtgtggcgc
     1141 ggtgaacgcc gtaacctttg accgggggcg cgccgtggga cacaacacgg atggaagggc
     1201 catccgggag accctggaga gcctcggggc cccggaggga ccagccctcc tcctgggcac
     1261 cggcggagtg gcccgggccg ccgccggtgc cttagcggac cggggggcag acccggtgta
     1321 catcgccggg cgcgacctgg agaaggggaa aaacctcgtt cgggagctcg tctcccgggg
     1381 gataagggca aattttgagg tggttcccct cgatgccctc cccgggaatt gtctcctggg
     1441 ggtgcacgcc acatccctgg ggctcccgga aaaccccttc ccccctacca tcctggaggc
     1501 gatgatgggg gcccttcggg aggggggcgc cctcctcgac ctggtctact cccccggagg
     1561 gacgcccctg gtcgccgccg cccgaaggcg cggcctgaac gcccgggatg gcacggaggt
     1621 gctgctccgc cagggggccg cctccttcac gctcttcacg ggcaaggccg cccccttgag
     1681 ggccatggcc agagccctgg ggatcgggga gatcccgggg gtggcatcat gacgtggcgc
     1741 ttcctcacca gcggcgaatc gcacggccgg gggctcctcg tggtggtgga cggcctgcct
     1801 gcggggctcg agctatgcct cgaagacctc gcctcggagc tcgcccggag gcggcggggg
     1861 cacggacggg gcccccgcat ggcgctggag cgggacgaga tccaggtgtg gggcgggctg
     1921 agaaacggtc gcaccacggg ggcccccgtg ggggtcgccc tggcaaacgc ggaggccgac
     1981 caatgggcct cggccatgca cccctggcac acctcccccg aggcggtgga ggcccggcgc
     2041 gtcaccgccc cccggccggg gcacgccgac ctggcggggg ccctcaaata ccgccacagc
     2101 gacatgcgcg acgtcctgga gcgggcctcg gcccgcacca ccgccccccg caccgtggcg
     2161 gggaccttgg cccgacgcct cctcgcccac tgcggcatcg ccgtccgggg ggccgtgctc
     2221 tccatcggag gggtctcggt gcccccgcct cgggacgacg ccggatggga gcgagctcgg
     2281 acctcgcccc tgggatgcgc cacggaggag ggagaggcga tgctcgcggc ccccatcgac
     2341 cgcgcccggg gggaagggga caccttgggg ggcaccttcg tcgtctcggt gcgaggggtc
     2401 ccggcgggcc tcggcagcgg cgtggagtgg gaccgacgcc tcgacgcccg cctcggggcg
     2461 gccctgctct cgatccccgg catcaagggg gtggagatcg gagaggcctt cgcggcggcg
     2521 gccctcaccg ggagcgctgt gcacgacccc atcgacggcc gaggaggcga gtggcggcgg
     2581 ccgagcaacc gcgccggagg gatcgaagga gggatgagca acggggaaga ggtcgtgatc
     2641 cggggagcca tgaagcccat ccccacccta aggaagcccc tgccctcctt cgacgtggcc
     2701 acgggaagga gcggcgccgc ccacgccgaa cggagcgacg tgtgcgccgt ggcggcggcc
     2761 tgcgtcgtgg gagaggccat ggtggcctgg gtgcttgggg gcgagctggt ggagcagttc
     2821 ggcggcgaca ccgtagagga tctccgggat cgcctggagg cccatcgacg ccgggtgaag
     2881 gggtggatgc tatgacgacg cccctgggat cgccccctct ctccttcaac cgccccctct
     2941 tcatcggggg gttcatggcc tccggcaaga gcacggtggg gcgcctggcg gcccgccttc
     3001 tgggggtgcc cttcctcgac acggaccgca tgatcgaaga acgggcccgg aaatccgtgg
     3061 gggagatctt cgcccaggaa ggggaggggg cctttcgctc gcaggaggcg gccgtggtgc
     3121 gcgaaattct ttccctgcga agcgtcgtgg tggccctggg aggggggacc ctggaagacc
     3181 cggggctccg ggacgagatc gccgaaaagg ggatcttggt gaccctcgcc gtctccgccg
     3241 aaacggcccg gcgccgaggg gcttccgggc cggatcgccc cctgctccgg ggcgacatcg
     3301 ccgccctctt ggcctcccgg gccccggggt acgccgcagg gcgtttttcc ctggacacgg
     3361 agcacttctc ccccgaggcc acggcaaaag agctggtgac acgacttttt ggcgacctcc
     3421 caccggccct cccccctcgg gggccgacac gtctggcccc tttccccggc ggaggatcgg
     3481 aggtgttcat aggcccgggg atcctccggg aaatcccggc cctggtcccc ggaggagatc
     3541 gagcccgcat cgtgggagat gggatcaccg ctcccctttt tgctcccctc atggcccttg
     3601 cgggagaggc gtacctcctg ccccggggcg agggggccaa gaccctgtac caggttcagg
     3661 cgctctacga tcacttcgcc cgatcttccg tggaccgcac cactccggtg gcggccctcg
     3721 gcgggggcac ggtgggggac tgcgcggggt ttgccgccgc cacctggatg cgcggcgtcc
     3781 ctctgattca gtgccccacc accctcctgg ccatggtgga cagcgccatc gggggaaaga
     3841 ccggggtgaa catcccccag ggaaaaaacc tggtgggggc ctttcatcac cccatcgccg
     3901 tggtggcaga cgcagagtgt ctccaaactc ttcccgccga ggagttccgt caagggttgg
     3961 ccgaggcggt gaagtacgcc gtgggagaag accagggcct cctggaggag ctggaaaagt
     4021 cccgaggatc catcctggcg cggcaccccg ccgccctcac ccacctggtg gagcgctgcg
     4081 tccgactgaa gctcgccgtc gtcgccgaag atggggacga gacgcgggga atccgggatc
     4141 gcctcaacct cggccacacc gtgggacacg ccatggaggc ggcgtcggac tttcggtgga
     4201 aacacggcga tgccgtggcc gctggccttg ccgtcgccct gaccctggga ggacggctcg
     4261 gaagttgttc ttcagctttt gttgcccggg gacgggagct cctcgaggcc ctggggctcc
     4321 cctggcgccc cgacctcccc tggggggagc ttttgccctt tctggggctc gacaagaagt
     4381 tccactgcgg gcagtgccgc ttcgtcctgc ccgtggaggg ggcgcggtgc aaggtccgc
//
LOCUS       DAOOER010000108         4417 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_18650_length_4417_cov_8.991059, whole genome shotgun sequence.
ACCESSION   DAOOER010000108 DAOOER010000000
VERSION     DAOOER010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18650_length_4417_cov_8.991059"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1303
                     /gene="infB"
                     /locus_tag="PL162_09180"
     CDS             <1..1303
                     /gene="infB"
                     /locus_tag="PL162_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HQF92229.1"
                     /translation="GAQVTDIAILVVAADDGLKPQTVEAMNHAKAAGVPIIVAVNKID
                     KPDAKPDRVRQQLSDHGLVPEEWGGETVMVEVSAKKGIGVEQLLEMVVLVAELRELRA
                     DPTVDPVGTVIEAKLDKGKGPVATVIVQEGTLMRGDIVLTETRWGRVRAMLDDRGKPV
                     DLAGPSIPVEIMGLDGVPQPGERFRKVMHEKEARDQVDVVIQEQKVASSTVKRLTLEE
                     IFDQMQTGSAPLLNLVLKCDVIGSLEALRGSLQKLATDEVGINIVHEGVGRISESDVM
                     LAAASNAIIMGFNVRPDSNATKIAEGEGVQVRLYRVIYDIIDDVRAALEGMLAPTLRE
                     QILGEVEIRAVFKVPKSGKVAGCYVTRGVIKRNARVRLIREGVVSWEGTLSDLKRFKD
                     DVREVAAGYECGLSFNNFQDFREGDVVEAYEIVQEKRTLDG"
     gene            1333..1623
                     /locus_tag="PL162_09185"
     CDS             1333..1623
                     /locus_tag="PL162_09185"
                     /inference="COORDINATES: protein motif:HMM:NF016351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HQF92230.1"
                     /translation="MEVDVFWSRSLKDRRQVMRSLVDHLRHHWNVSVAELSPEDSWRH
                     GSVAICCAGNSLVAVREKLDALGSYFTHREEEGDFYIMSQRFEVVGYDELSR"
     gene            1604..2032
                     /gene="rbfA"
                     /locus_tag="PL162_09190"
     CDS             1604..2032
                     /gene="rbfA"
                     /locus_tag="PL162_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HQF92231.1"
                     /translation="MTSFRVERLNKEMQRDISEILRLRIKNDLASAAIITGVDCSRDL
                     SSAKVFYLTMRPEDRKAVGDLLSKMGGVIRGHLGKMLQIRQIPELRFILDRTEDRAQH
                     IDALLDRLAQERPSPETSSDLSLSREEEEGDSEEVEEGHP"
     gene            2029..3027
                     /locus_tag="PL162_09195"
     CDS             2029..3027
                     /locus_tag="PL162_09195"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HQF92232.1"
                     /translation="MTSMDDVLRALEESPSWLILSHERPDGDTVGSASALAEAAEHLG
                     KKWRWMGKDPVPESLAFLPHVGRFEVCNTLPRPLGLPEPVAVVAVDISAPGRAPENDP
                     LSGAKSQRLVVIDHHSDNSLGGPLALVDSQAAATGEIVWGLFQRAGWPLPLGAAEALF
                     VAIATDCGFFTYSNTTVDTLRVAMHLVQLGVRPAAIFDQVNARAPLGKLHLWGRALER
                     AVQWHQRRVGFSFLEDEDFLSTGTDRTHIEGLVNQLLLLEGTDFAVLLTEQQEMTSAS
                     LRSKGTINAQRLAHRWGGGGHPQAAGFRVRRPWEEVAEEVKKELGQMYFHGDSCSA"
     gene            3005..3934
                     /gene="truB"
                     /locus_tag="PL162_09200"
     CDS             3005..3934
                     /gene="truB"
                     /locus_tag="PL162_09200"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HQF92233.1"
                     /translation="MGILVLHKAPGERSTACVSLVRRVLKGAKVGHAGTLDSPAEGIL
                     VILVGASTRLSECAMALAKEYVTEVQFGETTSTDDAAGDLLERRPWGTPEELTRRLDE
                     KIPAFLGHRFQIPPAISAVHVDGQRAHDLARAGQDPGLSPRVVFVSSVQRRGPVTLDG
                     RVTLCVRCHKGTYIRSIVRDLGRALGCGAHVVTLERRRCGPFSLAQALSTEQVKNLGS
                     AELMERLLPDRHLAEAYPTYVPASPEAEEALRHGRSIPLYLLRRHRWGDHCPPPLVVL
                     LSRGMALGKRAFMDRRPWFVPHALIGIDGEGIR"
     gene            3931..>4417
                     /locus_tag="PL162_09205"
     CDS             3931..>4417
                     /locus_tag="PL162_09205"
                     /inference="COORDINATES: protein motif:HMM:NF018299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD synthetase family protein"
                     /protein_id="HQF92234.1"
                     /translation="MIAVLGAFDGFHRGHRSLFDQARHRASQGRDDWGVITFYPHPQA
                     VVSSRNFNYLFTEAERRILAMVFEVPRWEVLPFTRGLADMPPERFLDLLATRYPLRGI
                     VVGNNFFFGRGRSGDIDFLRRECVRRGWSCDVVSPMKQDGQAVSSTAIRRAILEGHVA
                     AA"
BASE COUNT          848 a         1225 c         1489 g          855 t
ORIGIN      
        1 gggggcccag gtgacggaca tcgccattct cgtggtggcg gcggacgacg gcctgaagcc
       61 ccaaaccgtg gaagccatga accatgccaa ggcggcggga gtgcccatca ttgtggcggt
      121 gaacaagatc gataagcccg acgccaagcc cgacagggtt cgtcaacagc tctcggatca
      181 cggcctggtc cccgaggagt ggggcggcga aacggtcatg gtggaagtat cggccaaaaa
      241 gggcatcggc gtggaacagc tcctggagat ggtggtccta gtggcagaac tgcgggagct
      301 gagggccgat cccacggtgg accccgtggg cacggttatc gaggccaagc tagacaaggg
      361 caaggggccg gtggccacgg tgatcgtgca ggaagggacc ttgatgcgag gggacatcgt
      421 gctcaccgaa acccgctggg ggcgcgtccg ggccatgctg gatgaccgag gaaagcccgt
      481 ggatctggcg ggccccagca ttcccgtgga aatcatggga ttggatggcg ttccccagcc
      541 cggagagcgt ttccgcaaag tgatgcacga gaaagaggcc cgggatcagg tggatgtggt
      601 gatccaggag cagaaggtgg cttcctccac ggtcaaacgc ctcaccctgg aggaaatttt
      661 cgaccagatg caaacgggat cggccccttt gctaaacctg gtactcaaat gcgatgtcat
      721 cgggtcattg gaagccctgc gggggtcgct gcaaaagctc gccaccgacg aagtgggaat
      781 caacatcgtc cacgaagggg tggggcggat ttccgagtcc gacgtcatgc tggcggccgc
      841 ctccaacgct atcatcatgg gatttaacgt gcgccccgat agcaacgcca cgaagattgc
      901 cgaaggtgaa ggggtccagg ttcgcctcta tagggtcatt tacgacatca tcgacgatgt
      961 cagggcggcc ctggaaggaa tgctggctcc cacgctacga gagcagatct tgggcgaagt
     1021 ggagatccgg gcggtgttca aggtgcctaa atcgggcaag gtggcgggat gctacgtgac
     1081 ccgaggagta atcaaaagaa acgcccgggt acgtctcata cgcgaaggag tggtgtcctg
     1141 ggagggaacc ctctccgatc tgaagcgatt taaggacgac gtgcgagaag tggcggcggg
     1201 atacgaatgt gggctaagtt ttaataattt ccaggacttt cgtgagggcg acgtggtgga
     1261 ggcctacgaa atcgtccagg agaagcgtac cctcgacgga tgagggcggt acccttcttc
     1321 ggggccggag tcgtggaagt ggacgtcttc tggtctcgga gcctgaaaga tcgacggcag
     1381 gtgatgcgtt ccctggtgga tcacctcagg caccactgga acgtctccgt ggcggagctg
     1441 agccccgagg actcctggcg ccatgggagc gtcgctatct gctgtgcggg aaactccctc
     1501 gtcgccgtga gggaaaaact cgatgccctg gggtcctact tcactcatcg ggaagaagag
     1561 ggcgattttt atattatgag tcaaagattc gaggtggtgg gctatgacga gctttcgcgt
     1621 tgagcgcctg aacaaggaga tgcagcggga catttcggaa attctacgac tgcgaatcaa
     1681 gaacgatctc gcatcggccg ccatcatcac aggggtggac tgtagccgcg acctgtcgtc
     1741 ggccaaggtc ttctacctca ccatgcgtcc ggaggatcga aaggccgtgg gcgaccttct
     1801 ctccaagatg gggggagtga tccgggggca tttggggaag atgcttcaga tccgccagat
     1861 ccccgagctc cgtttcatcc tggatcggac ggaagaccga gctcagcaca ttgatgccct
     1921 tttggatcgc ctggcccaag agcgtccttc cccggagaca tcgtcggatc tctccctctc
     1981 ccgagaggaa gaggagggag attccgagga ggtggaggag ggacatccat gacttccatg
     2041 gatgatgtcc tgcgcgccct ggaggaatcc ccatcgtggc tcattttaag ccacgagaga
     2101 cccgacgggg ataccgtggg tagcgcctcc gccctggcgg aagcggcgga acatctgggg
     2161 aaaaagtggc gatggatggg aaaggacccc gtgcccgagt cgttggcctt tctcccccac
     2221 gtggggcgtt ttgaggtctg caacaccctt ccccggcctt tgggcctccc cgagccggtg
     2281 gcggtggtgg cggtggatat cagtgccccc gggagggcgc cggagaacga tcccctttcc
     2341 ggggcaaagt cccagcgctt ggtggtgatt gatcaccaca gcgataactc cttaggaggg
     2401 cctctggcct tggtggattc ccaggcggcg gcaacgggag agatcgtctg gggcctcttt
     2461 cagcgcgcgg gatggcccct gcccttgggg gcagcggagg ccctttttgt ggccattgcc
     2521 acggattgcg gcttttttac gtattcaaac accaccgtcg acaccctgag ggtcgccatg
     2581 cacctggtgc agctcggggt gcgccccgcc gcgatctttg atcaagtgaa cgcgagggcc
     2641 cccttgggga aactccacct gtgggggcgg gccctggaac gggccgtcca atggcaccag
     2701 cggcgggtgg gattttcctt cctcgaagac gaggattttt tatcgaccgg cacggaccgg
     2761 acccacatcg aaggcttggt aaaccaattg ttgctcctgg aaggaacgga ctttgcggtc
     2821 cttctcacag aacagcaaga gatgaccagc gccagcctga ggtctaaggg aaccattaac
     2881 gcacagcgcc tggcccatcg gtggggggga ggaggtcacc cccaggcggc gggatttcgt
     2941 gtccgtcgcc cgtgggaaga ggtggcggaa gaggtgaaaa aagaactggg gcagatgtac
     3001 ttccatgggg attcttgttc tgcataaggc tccgggggag cgcagtacgg cctgtgtttc
     3061 cctggtgcgg cgggtgctta aaggagcgaa ggtgggacat gcgggaactc tggactcccc
     3121 ggccgaaggg attctagtca tcctcgtggg agcatcgacg cgcctcagcg agtgtgccat
     3181 ggctctggcc aaggagtacg tgacggaggt ccagtttggg gagaccacct cgacggacga
     3241 cgccgcaggc gatctcctcg aacggcgccc ctggggaact ccggaggagt tgactcggcg
     3301 tctggacgaa aaaattccgg cctttttagg gcaccgcttt cagatacccc cggcgatttc
     3361 ggccgtccac gtcgacgggc agcgggccca tgacttggcc cgagcggggc aggatcccgg
     3421 cctatcaccc cgggtggtct tcgtgtcgtc ggtccagcga cggggccccg tgacccttga
     3481 tgggcgggtc acattgtgcg tccgttgtca caaggggacg tacattcgta gcatcgtcag
     3541 agacctgggg cgcgcccttg gatgcggtgc ccacgtggta accctggaaa gacgccgttg
     3601 tggtcccttt tccttggcac aggccctatc cacggagcag gtaaaaaatc tggggtcggc
     3661 ggagcttatg gagcgtctgc tcccggatcg ccacctggcg gaggcctatc ccacctacgt
     3721 gcccgcaagc cccgaggcgg aagaggccct tcggcatggg cgctccatcc ccctttatct
     3781 cctgcggcgc catcgatggg gggatcactg tccccctccc ttggtggtgc tcctgtcccg
     3841 aggaatggcc ctgggcaaga gggccttcat ggaccgaagg ccttggttcg tcccccacgc
     3901 tctgatcggc atcgacgggg aggggattcg atgatcgccg ttcttggagc ctttgacgga
     3961 tttcatcggg ggcaccggag cctttttgac caggctcggc atcgggctag ccaagggcga
     4021 gacgattggg gagtcatcac gttttatccc catccccagg ccgtggtgtc gtcgagaaat
     4081 tttaattatt tattcactga agccgagcgg cggatcttgg cgatggtctt tgaggtccct
     4141 cgatgggagg tcctcccctt cactcgcggg ctggccgata tgcctcccga gcgcttcctg
     4201 gaccttttgg cgacgcgata cccccttcga ggtattgtgg tggggaacaa ttttttcttt
     4261 ggtcggggga ggtcggggga catcgacttc cttcggcggg agtgtgtccg ccgggggtgg
     4321 agctgtgacg tcgtgtcccc tatgaaacaa gacgggcagg ccgtcagcag tacggccatc
     4381 cgtcgggcga tcctagaagg ccatgtggcg gcggcga
//
LOCUS       DAOOER010000109         4417 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_18651_length_4417_cov_8.874828, whole genome shotgun sequence.
ACCESSION   DAOOER010000109 DAOOER010000000
VERSION     DAOOER010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18651_length_4417_cov_8.874828"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="PL162_09210"
     CDS             <1..849
                     /locus_tag="PL162_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQF92235.1"
                     /translation="NSIASAPVFENAGIPMVVTTATNPAVTVDPSSGQVRPMAFRPCF
                     IDPFQGTIAAQFAFEDLKARRAAILYDVGSDYSQWLAKYFEEAFKVAGGTIVAQEAFR
                     SEELDYRAQLGRIKEQAPDVIFIPTMQKEAALAAKQARDLGITAGLLGGDGWGSPDLM
                     DLGGSAIDGGYFVNITDLADPDIQKFLQAYREKFGADPVLPNPVMAQDALLLLVDAMK
                     RAGSTDGKALAAAMENTKDLPVTSGRLTMDPENHDPFNKPAVIQRVDVGQKAFVFVKK
                     FEPGRN"
     gene            948..1817
                     /locus_tag="PL162_09215"
     CDS             948..1817
                     /locus_tag="PL162_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQF92236.1"
                     /translation="MLQTLVTGLSIGGIYALMAVGYSLVFSVLNFSNFAHGAVIMLGA
                     YAGLGVVRLFPPHLALAVTGAAVAGALVAALNERLAYAALRRRRAPALYLMISAMGCA
                     VFLENLVYAVLGSRFYAFPEMIARSVVTLGGTSVSLLDAGALGFALLAILALHLFLTR
                     TVTGLAIRAGVSDMTMCALMGVNLDRLILVVFLLAGAFAGVAGAFLGVKYMVYPTMGW
                     ITNKAYIAAVIGGLGSLPGALLGGLLLGVTETVVSTYISSVLRDVFSFAILIALLLYL
                     PHGLLGREAEEKI"
     gene            1817..2674
                     /locus_tag="PL162_09220"
     CDS             1817..2674
                     /locus_tag="PL162_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQF92237.1"
                     /translation="MGYVVGICTLAGIQMIAVLGVTVFTGFTGLFSLGHAAFVALGAY
                     GAGISTFRWHLPFPLALGVGVATATLASFVIGVPALRARLRSDYFAIAILGFGEALRV
                     LLENLSLTNGARGLAGIPMATTPGAVLLCLLGSLLVVRRFLRSRLGSACIALREDPIA
                     AEMAGVSLFRTRLLSLALSAALCGLSGGLLAHYLSFIQPIMFTLTQSTMLLAAVIAGG
                     MGSVSGPLMASFLFTAIPEALRVAQMWRLVAFGLLLVLVMRFRPQGLLGFRELGDLLP
                     ARWRRGFLA"
     gene            2658..3428
                     /locus_tag="PL162_09225"
     CDS             2658..3428
                     /locus_tag="PL162_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQF92238.1"
                     /translation="MASLLECRGISRAFGGIRAVDDLSIAVDRGEILGLIGPNGAGKT
                     TVFNLISGFYAPSEGEILLEGSPLTGLRPDAIVRRGVARTFQNIRLFARQSALENVRT
                     PLLQRHPRGIVPTLLGTPSVRREEKRCTARAMELLDLVGLAPWSSAAAGTLPYGHQRR
                     LELARALASEPAVLLLDEPAAGMNPEETASLTALLATIAPAFDLTLLIIEHHMDLVMR
                     FCSRIAVLHFGRLLAEGSPAEIRASAAVREAYLGKKSC"
     gene            3422..4126
                     /locus_tag="PL162_09230"
     CDS             3422..4126
                     /locus_tag="PL162_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQF92239.1"
                     /translation="MLRIEELSVHYGVIRAVDRASLELPRGTLTALVGANGAGKSSLL
                     WALVGGIPRSGGEVYLDDRPLPTSPHEVVRRRVALVPERRRLFGELSVGENLRMGGYL
                     EPRESVRRQRQEEVFSLFPGLADRMKQQAGTLSGGEQQMLAIGRALMGGPRLLLLDEP
                     TLGLAPLLAEQVLLTLGTLRDRGMTLLLAEQNASLALEVADEAAVMETGRLGPPRPAR
                     ELAQDPAIRRAYLGEE"
BASE COUNT          645 a         1597 c         1390 g          785 t
ORIGIN      
        1 aactccatcg cctcggcccc ggtcttcgag aatgcgggga tccccatggt ggtcaccacc
       61 gccaccaacc ccgctgtgac ggtcgatccc tcctcggggc aggttcgccc catggccttt
      121 cgcccctgct tcatcgaccc cttccagggc accatcgccg cccagttcgc ctttgaggac
      181 ctaaaggccc gtcgggccgc catcctctac gacgtggggt cagactacag ccagtggctc
      241 gccaagtact tcgaagaggc cttcaaggtc gccgggggca ccatcgtggc ccaggaagcc
      301 tttcgctccg aggagctgga ctaccgggcc cagctcggcc gcatcaaaga gcaggccccc
      361 gacgtgatct tcatccccac catgcagaaa gaggcggcac tggcggctaa gcaggcccga
      421 gacctgggca tcaccgccgg gctcctcggg ggagatggct ggggaagccc ggacctcatg
      481 gacctcgggg gaagcgccat cgacggcggg tacttcgtga acatcaccga cctggccgac
      541 ccggacatcc agaagttcct ccaggcctac cgggaaaagt tcggcgccga ccccgtgctc
      601 ccgaaccccg tcatggccca ggacgccctg ctccttctgg tggacgccat gaagcgcgcc
      661 gggagcaccg atggcaaggc cctggccgcc gccatggaaa acaccaaaga cctccccgtc
      721 accagcggcc gcctcacgat ggaccccgag aaccacgatc ccttcaataa gcccgccgtg
      781 atccagcggg tggacgtggg acagaaggcc ttcgtcttcg tgaaaaagtt cgagcccggc
      841 cggaactgac cccgaaaccc cgcgcccctc aagccccccg ccgggacgac ccctaccacg
      901 gcgtcgcccc ggcggggcct ttagcctgag attaaggagg cccgcaagtg ctgcaaaccc
      961 tggtgacggg gctttccatc gggggcattt acgccctcat ggccgtggga tattccctgg
     1021 tgttcagcgt cctaaacttc agcaacttcg cccacggcgc cgtcatcatg ctgggggcct
     1081 acgccgggct cggagtggtg cgcctcttcc ccccccacct cgccctggcg gtgacggggg
     1141 ccgccgtggc gggagccctg gtggcggccc tcaacgaacg cctcgcctat gcggcccttc
     1201 ggagacgccg ggcccccgcc ctctacctca tgatcagcgc catggggtgc gcggtctttc
     1261 tggagaacct ggtgtacgcc gtgctgggat cccgctttta cgccttcccc gaaatgatcg
     1321 cccgatcggt ggtgaccctg gggggcacct ccgtaagcct cctggacgca ggggccctgg
     1381 ggttcgccct cctggccatc ctcgccctgc acctcttcct cacccgcacg gtgacagggc
     1441 tcgccattcg ggcgggggtg tcggacatga ccatgtgcgc cctcatgggg gtaaacctgg
     1501 atcgcctgat cctggtggtc ttcctcctgg cgggggcctt cgccggggtg gcgggggcct
     1561 ttttgggcgt caagtacatg gtctacccca ccatgggatg gatcaccaac aaagcctaca
     1621 tcgccgccgt catcgggggg ctgggaagcc ttcccggggc cctcctgggg ggactcctcc
     1681 tgggagtgac ggagacggtg gtctccacct acatctccag cgtgctccgg gacgtgttca
     1741 gcttcgccat cctcatcgcc ctgctgctct accttcccca cggcctcctg gggcgcgaag
     1801 cggaggaaaa aatctgatgg gctacgtggt gggcatctgc accctggcgg gcatccagat
     1861 gatcgcggtg ctgggggtga cggtctttac gggcttcacc gggctctttt ccctgggtca
     1921 cgcggccttc gtggccctgg gagcctacgg cgcggggatt tccacctttc ggtggcacct
     1981 cccctttccc ctggccctgg gagtgggggt ggcgacggcc accttggcga gcttcgtcat
     2041 cggcgtcccc gccctgcggg cccggctgcg gagcgactac ttcgccatcg ccatcctggg
     2101 cttcggcgag gccctccggg tcctcctgga aaacctgtcc ctcaccaacg gcgcccgggg
     2161 actggcgggg atccccatgg ccaccacccc cggggcggtg ctcctttgcc tcctgggctc
     2221 cctcctggtg gtgcgacgct ttctccgctc ccggctgggg tcggcgtgca tcgccctccg
     2281 ggaagacccc atcgcggcgg agatggccgg ggtatccctc ttccgcaccc gcctcctctc
     2341 cctggcgctc tcggccgccc tctgcggcct ctcggggggt cttttggccc actacctgtc
     2401 ctttatccag cccatcatgt tcaccctgac ccaatccacc atgctcctgg cggcggtcat
     2461 cgccggaggc atggggagcg tctccggccc cctcatggct tcttttcttt tcaccgccat
     2521 ccccgaagcc ctgcgggtgg cccagatgtg gcgtctggtg gcctttggcc tcctgctggt
     2581 cctggtcatg cgctttcgcc cccaggggct attgggcttc cgggagctgg gcgatctctt
     2641 gcccgcgagg tggcgtcgtg gcttccttgc ttgagtgccg ggggatctcc cgggccttcg
     2701 ggggcatccg ggcagtggac gacctttcta tcgccgtgga ccggggggag atcttggggc
     2761 tcatcggccc caacggcgcg ggcaagacca ccgtcttcaa cctcatctcg ggattttacg
     2821 ccccctccga aggggagatc ctcctggaag ggagccccct cacgggcctc cgccccgacg
     2881 ccatcgtccg ccggggcgtg gcccgaacct tccagaacat ccgcctcttc gcccgccaaa
     2941 gtgccctgga gaacgtgcgc acccccctgc tccagcgcca cccccggggg atcgtcccca
     3001 ccctcctcgg cacgccctcc gtgcgccgcg aggaaaagcg gtgcaccgcc cgggcgatgg
     3061 agctcctcga cctcgtgggg ttagccccct ggagttcggc ggcggcgggc acgctcccct
     3121 acggacacca aaggcgcctg gagctcgccc gagccttggc gtcggagccc gccgttttgc
     3181 tcctggacga acctgcagcg gggatgaacc ccgaggaaac ggcgtccctc accgccctgc
     3241 tggcgaccat cgccccggcc ttcgacttga ccttgctgat catcgagcac cacatggacc
     3301 tggtgatgcg cttttgctcc cgcatcgccg tgctccactt cggccgcctg ttggccgagg
     3361 gctccccggc ggaaatccgg gcctcggcgg cggtgcgaga ggcatacctg gggaagaaat
     3421 catgctgagg atagaagagc tgtcggtgca ttacggcgtc atccgggcag tggatcgggc
     3481 ctccctggag ctccctcggg gcaccctcac cgccctggtg ggagccaatg gcgcgggcaa
     3541 atcctccctg ctgtgggccc tggtcggagg gatcccccgg tcggggggcg aggtgtacct
     3601 ggacgaccgc cccctcccca cctcgcccca cgaggtggtg cgccggagag tggcgctggt
     3661 gcccgagcga cggcggcttt tcggcgagct ctccgtgggg gaaaatctgc gcatgggggg
     3721 ctatctggaa ccccgggaat cggtgcgccg ccagcgtcag gaagaggtct tttccctctt
     3781 tcccgggctg gcggatcgca tgaaacagca ggcgggaacc ctgtcggggg gagagcagca
     3841 gatgctcgcc atcggccggg ccctcatggg ggggccccgg ctcctcttgc tggacgaacc
     3901 caccctgggg ctcgcccccc tgctggcgga acaggtcctg ctcaccctgg gaaccctgcg
     3961 cgaccgggga atgaccctgc tcttggcgga gcaaaacgcc tccctggccc tcgaagtggc
     4021 cgacgaggcg gcggtgatgg aaacgggacg cctggggccc ccgaggcccg cccgagagct
     4081 ggcccaagac cccgccattc gccgggccta cctgggggag gaatgacccc tccgcccggg
     4141 cctcgtccct acggccgtct cccgggagca caagccggga gcgcgcacct tccagccggc
     4201 tctcttcagt cttttttgtt ttgcgccatc tcctaggctt ttgcccctac agccttttac
     4261 cctgcgtcgc cagcagaggg caagaatgcg agcccctatg gggggggtgt taaaaaacca
     4321 aagaccatcc attcccgcca tgatgattac tggcgtcttg caaaaaaaca cctcaatcca
     4381 tcagcgccgc cacctcgtcc ggggaaagtc ccgtcac
//
LOCUS       DAOOER010000110         4412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_18672_length_4412_cov_8.098233, whole genome shotgun sequence.
ACCESSION   DAOOER010000110 DAOOER010000000
VERSION     DAOOER010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18672_length_4412_cov_8.098233"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="PL162_09235"
     CDS             complement(<1..884)
                     /locus_tag="PL162_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HQF92240.1"
                     /translation="MTDVPFSPSAGAGAPPPDREGKGLNFLEQIIADDLKSGRVATVC
                     TRFPPEPNGYLHIGHAKSICLNFGLAESFGGRCNLRFDDTNPSKEETEYVESIIRDVL
                     WLGFKPSAICYASDYFDQFFAWALELLAAGKAYVDSQTAEEIRATRGTLTQPGTPSPY
                     RDRSVAENEELFARMARGEMEEGSVVLRARIDMAHPNLNMRDPVLYRILKRPHHRTGD
                     RWCLYPMYDFAHGFEDAIEGVTHSICTLEFQDHRPLYEWLLNNVSVPCHPRQFEFARL
                     NLSYTLMSKRKLLELVTS"
     gene            1140..2285
                     /locus_tag="PL162_09240"
     CDS             1140..2285
                     /locus_tag="PL162_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HQF92241.1"
                     /translation="MEREERTYLKEFRGVAIPFIAIFALLTVLVYTVTSPLFHALAWS
                     ALLSFFAMPLYRWFHRRVLGGHLANVAATLTTGAILLLIVLPAVGLGAVIANEFVRLY
                     ATFMENYLRAGGSFDIQELSAFLPKDYVAMVLPWFSKYPFLQDLAGKAGGWLAGLLAD
                     VSRGLLQQTLYVGWELVIIIVASFFMVRDGHIILDYIRDILPLPEVERSRFFERARVL
                     LQSVIYGVTLTAAVQAMLGTLAWWYLGLPSPLLFGVLMFILAMIPFVGTPVLLLPGAA
                     YLFFSGHPQAGLIMASWSLGVVSMVDNFIGPIFISEGSGAHILMVFMGVIGGLAAWGF
                     LGLFLGPLVLALFIFLLDSYRRTLGIQRRSHDGPEARNEELSPSLPQ"
     gene            complement(2363..4021)
                     /locus_tag="PL162_09245"
     CDS             complement(2363..4021)
                     /locus_tag="PL162_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92242.1"
                     /translation="MNKRAVVFGAIFSLGSLLLLGGCGSDSSAPRSQGPVPNPWLAAP
                     HYAITHFDSSQSDSTPYGPPPGTYQVDPTARPLSPGGPINIMTLATPHQDYMWGVGTD
                     HVAYVEAKAGAWRTLAHVDAPAYIAPGLGPIAPEILRAFGEMNPIGMTSTDMHLRLST
                     DFGGNYPARLVNGAYSVVDRDNILYANFGSGVYAFALNDPADPSKGIRIVHRIDDSRA
                     LQGENAPASVTIQGITLTYDGYLIVTFRNGVAVVGRDLDATKSHFVPFGPLEQVTNSV
                     AVDEKNGIYVASDKVMRKLVWTGTTLSERPEDGAWSSPYDAPEDAVPPIVKFGPGTGS
                     TPTLMGFGSDPDKLVVITDGVKRMKLVAFWRDEIPEGFVQRPGTASRRIAGQIDVTCG
                     LPASTPWIQSEQSVVVSGYGAFVVNNIPADADAMDIAGAHKILAVSLMGPIYTPSLGV
                     ERFQWHPDSDTWASVWRRPDVSSTSMIPIHSQSRHMALVNGYTAERGWEVTGMNWETG
                     VTVHQTFFGRPNLGNGAYAILEYLENGDLVFNSIVGPFRVSYGN"
     gene            complement(4227..>4412)
                     /locus_tag="PL162_09250"
     CDS             complement(4227..>4412)
                     /locus_tag="PL162_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014807320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HQF92243.1"
                     /translation="EIVQGLARAEASPVVRVTHPEAELTHEAAIGRIADDKIEGLMAK
                     GLDEEQAIDAIVAGLLR"
BASE COUNT          735 a         1324 c         1460 g          893 t
ORIGIN      
        1 tttgaagtga ccagttccag gagtttgcgc ttgctcatga gggtgtagct caggttcagc
       61 cgggcaaact cgaactggcg gggatggcag ggaacggaga cgttgttcag gagccattcg
      121 tacagggggc ggtggtcctg gaactccagg gtgcagatgg aatgggtcac cccttcgatg
      181 gcatcctcga agccgtgggc gaagtcgtac atggggtaca ggcaccaccg atcccccgtg
      241 cggtggtggg ggcgcttcag gatgcgatag agcacgggat cccgcatgtt caggttgggg
      301 tgggccatgt cgatgcgggc ccgaagcacg acgctcccct cttccatctc cccccgggcc
      361 atgcgggcga agagctcctc gttctccgcc acggaacggt cccgataggg cgacggggtg
      421 ccgggttggg tgagggtacc ccgggtggct cggatctcct cggccgtctg ggagtccacg
      481 taggccttgc ctgcggccaa aagctccaga gcccaggcga agaattggtc gaagtagtcc
      541 gaggcgtagc agatggcgct gggcttgaac cccagccaca ggacgtctcg gatgatggat
      601 tccacgtact ccgtctcttc cttcgagggg ttcgtgtcgt cgaagcgcag gttgcaccgc
      661 cctccgaagc tctcggccag gccgaaattc aggcagatag atttggcgtg gccgatgtgc
      721 aggtagccgt tgggctccgg ggggaatcgg gtgcacaccg tcgccactcg cccgctcttg
      781 aggtcgtcgg cgatgatctg ttcgaggaag ttcaggccct tgccttctct atccgggggg
      841 ggtgccccgg cacctgcgga gggagaaaaa gggacatcgg tcataaaaac gcacactcct
      901 ttcaaggcca cgccccgccc ccgggggctt atcgcttttc ctcctggttt ctagggaaat
      961 cgaaaggggg gtgggtcctc atctcgctgt attctggcac ttggggcgga aattgtaaat
     1021 tcgctatcgc accttttgcc gaagccccta taatggggag ggggcgatcc ccccgaagat
     1081 ggagaccctc cgaaggaatc ggcgatttct aggggggagc cgaaaggtgg gagctggctt
     1141 tggagcggga agaacggacg tatctcaagg agtttcgagg ggtggccatc cccttcatcg
     1201 ccatcttcgc cctgctcacc gtcttggtct acacggtgac gtccccgctg ttccatgccc
     1261 tggcctggtc cgccctgctt tcttttttcg ccatgcccct atatcgatgg tttcatcgcc
     1321 gagtcctggg ggggcacctg gccaatgtgg ccgccaccct caccacgggg gccatcctgc
     1381 tcctcatcgt cctcccggcg gtggggctgg gggcggtgat cgccaacgag ttcgtgaggc
     1441 tctacgccac attcatggaa aattatctta gggccggggg atcctttgac atccaggagc
     1501 tgtccgcctt cctccccaag gactacgtgg ccatggtgtt gccctggttt tccaagtacc
     1561 cctttttgca ggacctggcg ggaaaggcgg gggggtggct ggcgggactt ctggccgacg
     1621 tatcccgggg gttgctccag cagaccctct acgtggggtg ggagctcgtc atcatcatcg
     1681 tggcatcttt tttcatggta cgcgatggcc acatcatatt ggattacatt agggatatcc
     1741 tgcccctgcc cgaggtggag cggagccgat tcttcgagcg ggcccgggtg ctccttcagt
     1801 ccgtgatcta cggagtcacc ctcaccgccg ccgtgcaggc catgttgggg accctggcgt
     1861 ggtggtacct gggcctcccc tctcccctgc tcttcggggt gctcatgttc atcctggcca
     1921 tgatcccctt cgtgggcacc ccggtgctcc tcctccccgg ggcggcctat cttttcttct
     1981 ccggccatcc ccaggcggga ctaattatgg cgtcctggag cctgggggtg gtgagcatgg
     2041 tggacaactt catcgggccc atcttcatct ccgaggggag cggggcccac atcctgatgg
     2101 tcttcatggg ggtcatcgga ggactggcgg cctggggctt tctgggcctt ttcctgggcc
     2161 ccctggtgct ggccctcttt atctttctcc tggatagcta tcgtcggacc ctgggcatcc
     2221 agcgccgctc ccatgacggc cccgaggcta ggaacgagga gctctccccc tccctgcccc
     2281 agtaaagtcg cgatacgaaa ggcggggggg gctaaagccc ccccgcgtcg cttggccggg
     2341 gcgatttttc cgaaaagggc ctttagttcc cgtaggaaac ccggaagggt cccacgatgg
     2401 agttgaatac gaggtcccca ttttccaggt actccaagat ggcgtaggcc ccgttgccga
     2461 ggtttggtct gccgaagaaa gtctggtgca ccgtgacccc cgtctcccag ttcatgccgg
     2521 tgacctccca tcctctttcg gcggtgtagc cgttcaccag ggccatgtga cgggactggc
     2581 tgtgaatggg tatcatgctg gtggaagaca cgtcggggcg ccgccacacg gaggcccagg
     2641 tatcgctgtc agggtgccac tggaagcgtt ccacgccgag gctcggagtg tagatggggc
     2701 ccatgaggga caccgccaag atcttgtggg ctccggcgat gtccatggcg tccgcatcgg
     2761 cggggatgtt attcaccacg aaggccccgt atcccgacac caccacggac tgttcggact
     2821 ggatccacgg cgtggaggcc ggaaggccgc aggtgacgtc gatctgccct gcgatgcgtc
     2881 gtgaggcggt ccccggacgc tgcacgaacc cctcggggat ctcgtcgcgc cagaatgcca
     2941 cgagcttcat ccgcttgact ccgtcggtga tgaccacgag cttatcgggg tcagagccaa
     3001 agcccatgag ggtgggggtg gagcccgtgc ccgggccgaa ctttactatg ggcggaacgg
     3061 cgtcctccgg ggcgtcgtag gggctagacc aggcgccgtc ttcggggcgc tccgagaggg
     3121 tcgttcccgt ccatacgagc ttgcgcatga ccttgtccga ggccacatat atgccatttt
     3181 tctcgtccac cgccacggag ttggtcacct gctccagggg gccaaagggg acgaagtggg
     3241 acttcgtcgc atcgaggtct cggcctacta cggccacgcc gtttcggaac gtgacgatga
     3301 gatatccgtc gtaggtgagg gtgatcccct ggatcgtcac cgacgccggg gcattctctc
     3361 cctgaagggc gcgggaatca tcgatgcggt gaacgatgcg gatgcctttg gatggatcgg
     3421 cgggatcgtt aagggcgaag gcgtagacgc cgctgccgaa attggcgtag aggatgttat
     3481 cccggtccac cacagagtac gccccgttca ccaggcgggc ggggtaattc cctccgaagt
     3541 ccgtgctgag gcggaggtgc atgtcggtgg aggtcatgcc gatggggttc atctctccga
     3601 aggcccgaag gatctccggg gcgatgggcc ccaggccggg ggcgatatag gccggggcat
     3661 cgacgtgggc cagggtgcgc catgccccgg ctttggcttc cacgtaggcc acgtggtccg
     3721 ttcccacgcc ccacatgtag tcctggtggg gggtggccag ggtcattata ttgatggggc
     3781 ctccgggaga gaggggacgg gcggtggggt ccacctggta cgtgcctggc ggagggccgt
     3841 agggagtgga atcgctttga gaggaatcaa agtgggtgat ggcgtagtgg ggcgccgcca
     3901 gccaagggtt gggtacgggg ccctgagatc ggggcgccga ggagtccgaa ccgcatccgc
     3961 ccaagaggag aaggcttccc agggaaaaaa ttgcaccaaa aaccaccgca cgcttgttca
     4021 tcgtaccacc tcctggtcaa tatccctctc cgtgagaata aagaagaggc ccacccccgc
     4081 ttctctgacg cctggagccg ggggcaacag attcttaaat aggctatgcc agagagcaat
     4141 ctttgtctac gggccgatga actccctccc ccgggatcca aaggtgtccg gggtgctccc
     4201 ggatctcacc gccccaagag gcccttctac cgcaaaagcc ccgccacgat ggcgtcgatg
     4261 gcctgctctt cgtccaaccc cttggccatg agcccttcga tcttgtcgtc ggcgatgcgg
     4321 ccgatggcgg cctcgtgggt gagctccgcc tcggggtggg tcacccgcac cacgggggac
     4381 gcctcggccc gggcgaggcc ctggacgatt tc
//
LOCUS       DAOOER010000111         4407 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_18691_length_4407_cov_9.383961, whole genome shotgun sequence.
ACCESSION   DAOOER010000111 DAOOER010000000
VERSION     DAOOER010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18691_length_4407_cov_9.383961"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="PL162_09255"
     CDS             <1..447
                     /locus_tag="PL162_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92244.1"
                     /translation="PQPQPQPQSPQPLLQPQPYSVPAQGSGGGTTAGPGPALPLPGGE
                     GVMNTSEEMVIIDFYPVDEGTKNQLRMLHKGGYFSYSNGDFRKAYMAFTKALEMYEGN
                     YLDAYWAARAAHKSGQKGKMKELLQRALTINPDYVPAQNYLATYGK"
     gene            462..1295
                     /locus_tag="PL162_09260"
     CDS             462..1295
                     /locus_tag="PL162_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92245.1"
                     /translation="MGKLMRIGGVAAAMMLLLGSGALAGAIPKGKAVAVVIAETAGIL
                     SNAAVGTAETLVKQTLMQHGYPVVNEAQLAKIRKSKAAALALDGNVDAILSLGKKYGV
                     KIFVSGKATMHESRQNEFGLFTGTAAIAVQAYSATDGKYLFADTVMGKDLGYTPEEAG
                     QKALMAAARVMAENIVRGEGAAGDGTSGGDPATATAQTVMVVVSRVGGFNVANQILDA
                     CSHLPRTRSARITSFSGGTVAVEVLYQSTPRDLADSLSRRGLPINITGVRGNTVEGQG
                     Y"
     gene            1347..2048
                     /locus_tag="PL162_09265"
     CDS             1347..2048
                     /locus_tag="PL162_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92246.1"
                     /translation="MRRRMGGLLVLAALLGALLVTGAGAEVQLFVDKQTVRVDDPQLG
                     EKSILNDFNPKGYRTHNYLALAWNDRESWIYDLRTHQWMPLRDFAPVAGLLTDDYALV
                     WEPNRAAVFDARERRWIVSTMMLDQITTPLLSRGMAAVVSTREFVVYDPVLHEWQIMG
                     NVAPKQAALGDNLAVCWNTEDAIIYDTTMHQWLEKRGISPQACIVEDYKLSIYTVDTI
                     HIYDAMKHRWGQQPR"
     gene            complement(2272..2910)
                     /locus_tag="PL162_09270"
     CDS             complement(2272..2910)
                     /locus_tag="PL162_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92247.1"
                     /translation="MKKFFAGLALGTMALTLAVGGAWARDNLSLTFDLAALDRRETND
                     VRLAWTGSEFRMEALRGDLLWFQYFSLPKNAAFRNSFSLEAEVSSVQDRGVAGIALGN
                     RRIDLVFYVYLHDRHGYARIATADSSQAHFISEKTFSYPSTPFTLGVVYDVDGGVFTG
                     YVNGQSVLSISSTSYPRLTQISSVNSVALVGGSRHDQPSGVVGFRSFSLEAE"
     gene            complement(2952..3284)
                     /locus_tag="PL162_09275"
     CDS             complement(2952..3284)
                     /locus_tag="PL162_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92248.1"
                     /translation="MRRLAWALLVVLLWGVGAMGATDTFSYGDVRITPLSGNIFRCEG
                     TVTNQSDQFYQGACFNLFILDMNGKTLNTLMFCLDKVQPHSTRDFRLDFQHVNRDFQY
                     RLEFLQTY"
     gene            3665..4165
                     /locus_tag="PL162_09280"
     CDS             3665..4165
                     /locus_tag="PL162_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92249.1"
                     /translation="MIRLLKGSPGEKDPEGSYLLGVALIRAGDRAEAPGALKRAVATG
                     GSRRVTVGGKAGSLRAVAASELALLLAEGGQGEEIVRLQEALKDQRGTRVPVAGDDGI
                     PRGENRLGVLLRLAEARALAQEGREEELRELLTLLSLEKGPVPWKGEVRPLEEALGDL
                     QRAGPR"
BASE COUNT          810 a         1312 c         1572 g          713 t
ORIGIN      
        1 ccccagcccc agccccagcc ccagtctccg cagcccttgc ttcagcccca gccctattcc
       61 gtccccgccc agggaagcgg cggtgggacg acggcggggc cgggtcccgc ccttcccctg
      121 ccggggggag agggtgtgat gaacacctcg gaggagatgg tgatcatcga cttctacccc
      181 gtggacgagg ggactaaaaa tcagctccgc atgctgcaca aagggggata tttcagctac
      241 tccaacggcg atttccgtaa ggcctacatg gcatttacca aggccctgga gatgtacgag
      301 gggaactacc tggacgccta ctgggcggcg cgggcggcgc acaagtcggg gcagaagggg
      361 aagatgaaag aactgctcca gagggctctg accatcaatc cggactacgt tcccgcccag
      421 aactatctgg ccacctacgg gaaataaggg gggatcggag catgggtaag ctcatgagga
      481 tcgggggcgt ggcggcggcg atgatgctgc tcctgggatc cggagcgctg gcgggggcca
      541 tccccaaggg taaggccgtg gcggtggtca tcgccgagac ggcggggatc ctgtccaacg
      601 cggcggtggg gacggcggag actttggtca agcagaccct gatgcagcac ggctatccgg
      661 tggtgaacga ggcccagctc gccaagatcc gcaagagcaa ggcggcggcc ctggcgctgg
      721 acggcaacgt ggatgccatc ctaagcctgg ggaaaaagta cggcgtgaag atcttcgtct
      781 ccggcaaggc caccatgcac gaatcccggc agaacgagtt cggcctcttc accggcacgg
      841 cggccatcgc cgtccaggcc tattccgcca ccgatggcaa gtatctcttt gccgacacgg
      901 tgatgggaaa ggacctgggg tatacgccgg aggaggcggg gcagaaggcc ctcatggcgg
      961 cggcccgggt catggcggaa aacatcgtcc ggggcgaagg ggcggcgggg gatggcacct
     1021 ccgggggcga cccggccacc gccacggccc agaccgtgat ggtggtggtg tcccgcgtgg
     1081 gagggtttaa cgtggccaac cagatcctgg acgcctgcag ccatctcccc cggacccgct
     1141 ccgcccgcat cacctccttc agcgggggca ccgtggcggt ggaggtgctc taccagagca
     1201 cccctcgaga tctggcggat tccctctccc gaagggggct tcccatcaac atcacggggg
     1261 tgcggggaaa cacggtggaa ggacaggggt actaggcttc ggcggccgga gcagcggaag
     1321 aatcgatgaa aggacggtgg cggaaaatgc gacggaggat ggggggactt ttggttctgg
     1381 cggctctcct gggggctctt ctggtgacgg gggccggggc ggaggtccag ctcttcgtgg
     1441 acaagcagac cgtgcgggtg gatgaccccc agctggggga gaagtcgatc ctcaacgact
     1501 ttaaccccaa ggggtaccgt acccataact acctggccct ggcctggaac gaccgggaga
     1561 gctggatcta cgacctgcgc acccaccagt ggatgcccct gagggacttt gcccccgtgg
     1621 cggggctcct cacggacgac tacgccctgg tatgggagcc caaccgggcg gcggtcttcg
     1681 atgcccggga gcggcgttgg atcgtgagca ccatgatgtt ggaccagatc accacgcccc
     1741 tgctctctcg gggcatggcg gcggtggtct ccacccggga gttcgtggtc tacgaccccg
     1801 tgctccacga gtggcagatc atgggaaacg tggcccccaa gcaggcggcc ctgggggata
     1861 acctggcggt gtgttggaac acggaagacg ccatcatcta cgacaccacc atgcaccagt
     1921 ggctggaaaa gcgcggcatc agtccccagg cgtgcatcgt ggaagactac aagctgagca
     1981 tctacactgt ggataccatc cacatctacg acgccatgaa gcaccgctgg gggcagcaac
     2041 cccggtagcc ccccgggcgt gccccgcaag accactggtc ccggctcccc ccgagtcccc
     2101 tcggggggag ccgggaccag ctcggtcgga gccttcccga tttttagctt tcttttgccg
     2161 ggatcgccca tctttcatcg ggctttgccg cgaaatgtgg gccacgatgg cgatctttct
     2221 cctggaggag gacatcgaag gggcccccgg gggggcggac ctcgccggga actactccgc
     2281 ttccagggag aacgaccgga accccaccac tcccgagggc tggtcgtggc ggcttccccc
     2341 caccagggcc acggagttga cggaagaaat ctgggtgagg cgagggtagg aagtggagga
     2401 gatggatagg acgctttggc cattcacgta cccggtgaag acccctccgt cgacgtcgta
     2461 gacgaccccc agggtgaaag gggtggaggg gtaagagaag gtcttctccg agatgaagtg
     2521 ggcctgagag ctgtcggcgg tggcgatgcg ggcgtatccg tgacggtcgt ggaggtagac
     2581 gtaaaacacc aggtcgatcc ttcgatttcc cagggcgatc cccgccacgc cccggtcttg
     2641 caccgaagac acctcggcct ccagggagaa actgttgcgg aaggcggcgt ttttgggcaa
     2701 gctgaagtac tggaaccaca ggaggtctcc ccgtagggcc tccatgcgga actcgcttcc
     2761 cgtccaggcg aggcgcacgt cgttcgtctc ccggcggtcc agggcggcga ggtcgaaggt
     2821 gagggacagg ttgtcccggg cccaggcccc tcccaccgcc agcgtcaggg ccatcgtccc
     2881 cagggccagg cccgcgaaga attttttcat ggcatattcc tcccttttgt cctatgctcc
     2941 cctcggggcc cctagtaggt ctggaggaac tccaggcggt actggaagtc ccggttcacg
     3001 tgctggaagt ccaggcgaaa gtcccgggtg ctatggggtt ggaccttgtc cagacagaac
     3061 atgagggtgt tcagcgtctt tccgttcatg tcgaggatga agaggttgaa gcaggcccct
     3121 tggtagaact ggtcggactg gttcgtgacg gtgccttcgc agcggaagat gttccccgaa
     3181 agcggcgtga tgcgtacgtc gccgtaggag aacgtgtccg tggcccccat cgccccgacg
     3241 ccccaaagca aaacgacgag aagcgcccat gccaatcttc gcatgatccc cacctccaat
     3301 tttttatggt cccttttctg gggtttcgca acgctccccc gggggctatc atggcgcgtt
     3361 atttccctga cgtcaagagc gggtagcagt tggctttcgt cccccgcgcc gctaaaatag
     3421 ggagtgatcg tccgagaaaa gagttcttgg ggatgggagg cgatggatca tgactaaggg
     3481 ggcaaaaatc gggggagggc tagcggcggc cctgctcctc ctcctggggg cggggatggc
     3541 cctggggtga tcctccggcg gggggagctc cggaggagct tccgagggcc aggggggaaa
     3601 aaaagatagc ccctcggccc tggaccgggc ctgggaagcc tacggccggg ggcgttacga
     3661 cgacgtgatc cgcctcctga aggggtctcc gggggaaaaa gaccccgagg gctcctatct
     3721 cctgggcgtg gccctgatca gggcgggaga ccgggccgag gctccggggg cgttgaagcg
     3781 cgcggtggcc acggggggat ctcggagggt gacggtgggg ggcaaggcgg gatcccttcg
     3841 ggccgtggcg gcgtcggagc tcgccctcct cttggccgag ggcgggcagg gggaagagat
     3901 cgtccggctg caggaggccc tgaaggacca gaggggcacc cgggtgcccg tggcggggga
     3961 cgatggcatc ccccgggggg agaatcggct gggggtgctc ctgaggctgg cggaggcccg
     4021 ggccttggcc caggaagggc gggaggagga gctgagggag ctcctgacct tactgtccct
     4081 ggaaaagggt cccgttccct ggaaggggga agtgaggcct ctggaggagg ccctgggcga
     4141 cctccagagg gcggggcccc ggtaaaaggc ctccctcagg cggcgtttat tttgggtagc
     4201 cccgggcccc gaccggagca cggaccccgg ccctcaggcg gaagaggggc gggacaaggg
     4261 ggcccggtgg gcccacggga gggatgcaca tggcaaagga cgagttgccc acccaggtgg
     4321 acgtggacga acttttcgac ctggcggcgg gggaccccga aatcgtgggg gagatcctgg
     4381 tggccttcca agaggaatac ccccggc
//
LOCUS       DAOOER010000112         4322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_19126_length_4322_cov_6.862667, whole genome shotgun sequence.
ACCESSION   DAOOER010000112 DAOOER010000000
VERSION     DAOOER010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19126_length_4322_cov_6.862667"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="PL162_09285"
     CDS             <1..688
                     /locus_tag="PL162_09285"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HQF92250.1"
                     /translation="LRPLLPPGAQDFLLHLSDTDLESGKDALVASFQQLGPWRHGAFL
                     GLQVLQVFFAPIPGQLTGFLGGFLFGFWPGLAMTMGGLMLGSGAAMLFSRKLGRPFVR
                     RVVGEKLFAKFGYLIEGGGLFSYFMLFLLPALPDDAICFVAGLSSLSLTKLLGVCLLG
                     RLPGMAVLSLAGASVGWNTDLATGVFAGAMGVSVVIWLYQEEIERWYHAHTEGGRPSS
                     DVPNKDRDES"
     gene            708..1079
                     /locus_tag="PL162_09290"
     CDS             708..1079
                     /locus_tag="PL162_09290"
                     /inference="COORDINATES: protein motif:HMM:NF015648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HQF92251.1"
                     /translation="MTYTLEVQHRPAWRSFWYAWLLCWLVLPLVYIVYKRYSTLLTVK
                     DDEVSFEDGFFSKSSTEIKIRDIKSIEVHQTLLDRLLGVGELRLATSGTAGWEVTVNH
                     LLDPHAIREDIQRRRGDENPS"
     gene            1200..1571
                     /locus_tag="PL162_09295"
     CDS             1200..1571
                     /locus_tag="PL162_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQF92252.1"
                     /translation="MELHIFRAADGAPYITKDESSIVEIFHPATLPGIPYSVAEATVP
                     PGGRTVPHHHERSLETYYVLEGEGVLLVGSERVALKTGDAALLPPGTPHAVEASARGL
                     RFLCICHPPYDHGQTVLEEEA"
     gene            complement(1538..1816)
                     /locus_tag="PL162_09300"
     CDS             complement(1538..1816)
                     /locus_tag="PL162_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92253.1"
                     /translation="MFVLVIGGTEVCRIPGISMAGSSPHAVPFTAPADAEVLWWGRGG
                     GVVRRVPGSSRSPDDLPHGGTLRGHDLRRVRVGSRLRDQASSSSTVWP"
     gene            2031..3194
                     /locus_tag="PL162_09305"
     CDS             2031..3194
                     /locus_tag="PL162_09305"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HQF92254.1"
                     /translation="MWRGRLPREILRETRESYVSRETSHLRRDEEEKGERIDCALGTN
                     PLGVAPAVRAFLQRPQEWDPAAYPSAVGETLREELAAYLGAEVTPEQIVLGHGSFDVL
                     THLMRLLLPAGSLLGGVSPQFTDVPLQVMLNNVRYHPVVLRPPFFEAEEESWMRAARI
                     HPHVLYVDRPNNPTGQVMPRNVLESLCALCARHGAWVVADEAYGEYLPLEESAIQIPA
                     PNLVVTRSFSKAWGLAGVRVGYGVVRDPELLEIFRKTQPPFSLGTPGMVLASVALGDP
                     SFLETTRRYVRTAKARLLEALTRPPELVPSATDERTPILVLSSHRGNLFDRLNRAGIA
                     TEPGTGYMDLDERAVRLRVPPPDELEPLLRRLHSVGVALEAEGPLPGRSLADP"
     gene            3368..>4322
                     /locus_tag="PL162_09310"
     CDS             3368..>4322
                     /locus_tag="PL162_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HQF92255.1"
                     /translation="MRAVCIHGHFYQPPREDPWFDEVLADPSAAPAHDWNDRVAMECY
                     RPNRAARLMDGAGRIVCIMDNYRHLSFNFGPTLHRWIARHDPVLDARLRASDGEAERE
                     LGAGGALAQAYNHMILPLASARDLRTQVRWGAADFRARFGRRSRGMWLPETAVSTSVL
                     EALAEEGVEFTILASGQCAAVRPPRGEWTPVSGGSVDVTRPYTVPLPSGKHVTVVFYQ
                     GSVSHDIAFGGLLDSGDRFAEYLLHQLPGGDEPRLLVVATDGETYGHHHRFGEMALAR
                     AFQVLASSREALLTNVGAFLQLHPPTWEARIVENSSWSCAHG"
BASE COUNT          667 a         1395 c         1460 g          800 t
ORIGIN      
        1 cctgcgcccc ctgttgcccc cgggggccca ggactttctt ttgcaccttt ccgacacgga
       61 cctggagagc ggcaaggatg ccctggtggc ctcttttcaa cagctgggcc cctggcgcca
      121 cggggccttt ttgggcctcc aggtgctcca ggtgttcttt gcccccattc cggggcagct
      181 cacgggcttt ctggggggat ttctcttcgg cttctggccc ggcctggcca tgaccatggg
      241 gggcttgatg ctgggctccg gcgccgccat gctcttttcc cgcaagctgg ggcgcccctt
      301 cgtgcgccgc gtggtgggag aaaagctctt cgccaagttc ggctatctca tcgagggcgg
      361 ggggcttttc agctacttca tgctctttct ccttcccgcc ctgccggacg acgccatttg
      421 cttcgtggcg ggactttcct ccctgtcgct gacgaaactg ctgggggtct gcctgttggg
      481 gcgccttccg ggcatggcgg tcctttccct cgcgggggcc tcggtggggt ggaatacgga
      541 tctggccacg ggggtctttg ccggagccat gggcgtttcg gtggtgatct ggctctatca
      601 ggaagagatc gaacggtggt atcatgccca tacggagggg ggacgccctt cctccgacgt
      661 gccgaacaag gaccgagacg agagctgacg aaggaggggg tttggagatg acgtataccc
      721 tggaggtgca acaccgcccg gcgtggcgga gtttctggta cgcgtggctc ctgtgctggc
      781 tggtgcttcc cttggtgtat atcgtgtaca agcgctattc gaccctgctc acggtgaagg
      841 acgacgaggt gtcctttgag gacggatttt tctccaagtc ttccacggag atcaagatcc
      901 gggacatcaa atccatcgaa gtgcaccaga ccctgctgga ccgtcttctg ggggtggggg
      961 aactccggct ggccacgtcg ggcacggcgg gatgggaggt caccgtgaac cacctcctgg
     1021 atccccacgc catccgcgag gacatccagc ggcgccgggg ggatgagaat ccttcctgat
     1081 ccctccggga ggccggtgtc cgtggcgctc cgggcggtgt gtcgggagcg cttttggtgt
     1141 gacgccgggc aggcccccag gcccgcccgg cgcattcttg ccgagtgagg agaagcgcca
     1201 tggaactcca catctttcgc gccgccgatg gggcgcccta catcaccaag gacgagagct
     1261 ccatcgtgga aatttttcac cccgccaccc tgcccggaat cccctacagc gtggcggaag
     1321 ccaccgtgcc ccccggaggg cgcacggtgc cccaccacca cgaacgaagc ctggagacct
     1381 actacgtgtt ggagggagag ggcgtgctcc ttgtgggatc ggagcgcgtg gcgctgaaaa
     1441 ccggagatgc ggcgctgctc cctccgggca ccccccacgc ggtggaggcg agcgcccggg
     1501 gactgcgctt tctctgtatc tgccaccccc cctacgatca cggccaaacg gtgctggagg
     1561 aagaggcctg atcccggagg cgggagccca ccctcacgcg ccggagatca tgtccccgta
     1621 gagttcctcc gtgcgggaga tcatccgggc tgcgggaact ccccggaacg cggcgtacca
     1681 caccgccccc ccgcccccac cagagcacct ccgcgtccgc cggagcggtg aagggcaccg
     1741 cgtgggggga actccccgcc atggagatgc cggggatgcg gcacacctcg gtgccgccga
     1801 tgacaagaac gaacatggcc gtggttcctt tcttttctcc ccgagtgttt ccccacgtct
     1861 tccgaaaagc gcacaggagg tcggccttct tcccggaggg agaacgtgtg gcgccggggc
     1921 gaacttcccc tataataaga gaaaagtggg cgaaggggag gcgcagtttt cctccgatcc
     1981 tctcctcgac accaccggag cgactccgtt ccgagggagg aggtgcggcc atgtggcgtg
     2041 ggcggttgcc ccgggagatc ctgcgggaga cccgggaaag ctatgtaagc cgggaaacga
     2101 gccatttgcg gcgggacgag gaagagaaag gggagcgcat cgactgtgcc ctgggaacca
     2161 atcccctggg agtggcgcct gcggtgcggg cctttcttca gcgcccccag gagtgggacc
     2221 cggcggcgta cccctcggcg gtgggagaaa cccttcgaga ggagctggcg gcctacctcg
     2281 gtgccgaggt gacgccggag cagatcgtgc tggggcatgg gtccttcgat gtgctgaccc
     2341 atctgatgcg gttgctcttg cctgcgggat ccctgctggg aggcgtctct cctcagttca
     2401 ccgatgtgcc ccttcaggtc atgctgaaca acgtccgcta ccaccccgtg gtgttgcgcc
     2461 cccccttctt cgaggccgag gaggagagct ggatgcgggc cgcccggatc cacccccacg
     2521 tgctctacgt ggaccgaccg aacaacccca cgggccaggt catgccccgg aacgtgctgg
     2581 agagcctctg cgccttgtgc gctcgccacg gagcctgggt ggtggcggac gaggcctacg
     2641 gcgaatatct gcccctggag gagagcgcga tacagattcc ggcgccgaac ctggtggtca
     2701 cccgatcctt ctccaaggcc tgggggctgg cgggggtccg agtggggtac ggtgtggtgc
     2761 gggatccgga acttctggag atcttccgca agacccaacc ccccttctcc ctcggcactc
     2821 cgggcatggt gctggcctcg gtggctctgg gagatccatc gtttttggag accacccggc
     2881 ggtatgtccg caccgccaag gcccggttgc tggaggcctt gacccggcca ccggaacttg
     2941 tgccctcggc gaccgacgaa cgcacgccca tcctggtgct ttcctcccac cggggaaacc
     3001 tttttgaccg cctgaatcgg gcggggatcg ccaccgagcc gggcacgggg tacatggatc
     3061 tggacgaaag ggctgtgcgg ttgcgagtgc ccccgccgga cgaactggaa cccttgctga
     3121 gacgcctgca cagcgtgggg gtcgccttgg aggccgaagg gccgttgccg gggcggagtc
     3181 ttgccgatcc ctgagccaac ccctcggtgt ccggtgaccc cgggtgccgg gaatttctgg
     3241 agcgttgtgg ctccgtgggg cgagcgggtg tttccgacga cggccccaga aaggggacct
     3301 tcccgagcgt tccgaaaggg cgcttttcga gtgataccga aacggagaac gagaggcggc
     3361 gggaatcttg cgcgcggtat gcatacacgg tcacttctat cagccccccc gggaagatcc
     3421 ttggtttgac gaagttctgg cggacccctc ggcggctccg gcacacgact ggaacgaccg
     3481 ggtggccatg gagtgctatc ggcccaatcg agcggcccgc ctgatggacg gggcggggcg
     3541 tatcgtctgc atcatggaca actatcggca tctcagcttc aatttcggcc ccacgctcca
     3601 ccggtggatt gcgcggcacg atcccgtgct ggacgcccgc ctccgggctt ccgacggtga
     3661 ggcggaacgg gagctcggcg cggggggcgc cctggcccag gcctacaacc acatgatcct
     3721 tcccttggcc tccgcccgcg atctccgaac ccaggtgcgg tggggcgcag cggattttcg
     3781 cgcccgtttc gggcgacgtt cccggggcat gtggctcccc gaaaccgccg tgagcacctc
     3841 cgtgttggag gccctggcgg aggagggcgt ggagttcacc attctggcgt cggggcagtg
     3901 tgccgccgtg cgcccccctc ggggagagtg gaccccggtc tccggtggct cggtggatgt
     3961 gacgcgcccc tacacggtgc ccttgccttc ggggaagcat gtcacggtgg tgttctatca
     4021 agggagcgtc tcgcacgaca ttgccttcgg cggccttctg gacagcggag accgttttgc
     4081 cgagtacctc ttgcatcaac tccccggagg agacgaaccc cgccttctgg tggtggccac
     4141 cgacggagag acctacggac accatcaccg ttttggagag atggctttgg cccgggcgtt
     4201 ccaggtgctg gcgtcctccc gagaggctct cctcaccaac gtgggggctt ttcttcagct
     4261 ccaccctccc acctgggagg cccgcatcgt ggagaacagc tcctggtcct gcgcccacgg
     4321 gg
//
LOCUS       DAOOER010000113         4300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_19266_length_4300_cov_8.902473, whole genome shotgun sequence.
ACCESSION   DAOOER010000113 DAOOER010000000
VERSION     DAOOER010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19266_length_4300_cov_8.902473"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PL162_09315"
     CDS             <1..350
                     /locus_tag="PL162_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92256.1"
                     /translation="ERDTVSRAARRVLRSYVDWGVLQESGVKGVYTAGAPLAVEDPRL
                     IAWLVEASLYARATDLAPLKGLLDSPGLFPFRIKPVHADGLGAVSSRLDFLRQGLNDD
                     LVMRRKQKTPSSP"
     gene            677..850
                     /locus_tag="PL162_09320"
     CDS             677..850
                     /locus_tag="PL162_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92257.1"
                     /translation="MGTGSPQFRTPPDAVIQRLSYSHMELIVDFDDDLKRAFYEIEGL
                     RGNWPVRELKRQI"
     gene            984..3788
                     /locus_tag="PL162_09325"
     CDS             984..3788
                     /locus_tag="PL162_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HQF92258.1"
                     /translation="MMPAVRKSPQESSPGPFGTEASAGVNEPVTCLGKAFPDDNARRE
                     HFLGLLREKLQDPEFRKIEGFPLGEDEDILALSDPPYYTTCPNPWIADFIRHYGKPYD
                     PNEPYRREPFAADVSEGKNDPIYNAHSYHTKVPHKAIMRYILHYTEPGDVVFDGFCGS
                     GMTGVAAQMCGDKAVVESLGYRVDEDGTISRRETDEEGKPLWRAFSQVGARRGVLADL
                     SPVATFITHNHTAPVDVHEFEREARHVLGEVEAECGWMYETLHSDGKTKGQINHTVWS
                     DVFICPECTGEIVFFEEAVDRDQGTVRADFPCPHCQARLTKRRMDRARETRFDGALNQ
                     SLSQIKQLPVLIDYTVDGRRAEKYPDDADLALIDKIDNGNIPYWFPAVRMPEGIESRR
                     NDPLGITHTHHFYSKRNLWILSSLRSRCTTGPMLLWFNSQLINVSKLNRYRPGVSFPY
                     NPLSGTLYMGSQTSEPCLFTAYKNKLNKLVKSLNSTNSPHIITTQSTTHISEMPSQSV
                     DYIFLDPPFGSNLNYSELSALWERWMKVWTNNALEAIENASQGKDLAAYRRLMAACFK
                     EAHRVLKPGRWMTVEFSNTKASVWNSLQAALTEAGFVVADVSALDKQQGSFKAVTTPT
                     AVKQDLVISAYKPGGGLMESFSLEEGPEGGVWDFVRAHLGYLPVVKKHGDTLQFIPER
                     DPRILFDRMVAHFVRKGHPVTLDSAEFQEGLARRFSYRDGMCFLPDQMADYDRGKMTA
                     GGMAPTPPSVSDESSAIAWLRQILQNRPQTFQDIHPLFIKELGGWQGREKPLELSELL
                     GQNFLCYDGKGAVPSPIYSYLSSTFEELRDRPKDDEDLRAQGKDRWYVPDPNKAGDLE
                     KLRERSLLKEFEEYKASATRKLKVFRMEAVRAGFKKAWQERDYATIIAVARKVPENVL
                     QEDPQLLMWYDQALTRSGGE"
BASE COUNT          928 a         1335 c         1244 g          793 t
ORIGIN      
        1 gcgagcggga taccgtttcc cgggcggcgc gccgtgtgct gcggtcgtac gtggattggg
       61 gcgtgctgca ggagagcggc gtcaaggggg tctacaccgc cggggctccc ctcgccgtgg
      121 aggatccccg gctcatcgcc tggctggtgg aagcctcgct ctacgcccgc gccacggact
      181 tggctccgct gaaaggtctc ctggacagcc cggggctttt ccccttccgg atcaagccgg
      241 tgcacgccga cggcctggga gccgtctcgt cccggctcga ttttctccgc cagggcttga
      301 acgatgacct cgtgatgcga cggaaacaga aaaccccgag ttcgccgtag gggcgaaatc
      361 cccggggccg gcgtaggggc gaaaaatacg ctcgccccta ccggataggg ctcatgtatt
      421 cgccgcacct cttgctttga agaataatca tgataaaatt gaacatacct atgagaaaca
      481 ccagacaatg ttattagatc gacgaaaact cttttcctgt gatgattgta aatcaacaaa
      541 aacttctgaa cacatcgtag ggacaaaaaa tatttctccc agcccgaaga gagatgggtc
      601 gcggacatga cacaaaacga aaaaatcccc caccgtcgtt cgatccgtta tcgtcgggac
      661 gaaagaaacc gagaaagtgg ggacagggtc cccacaattc cggacgccgc cggatgccgt
      721 catccaacgg ctttcgtaca gccatatgga gttgattgtc gattttgacg acgatctcaa
      781 gcgggccttc tacgaaatcg agggcctccg gggcaactgg ccggtccggg agttgaagcg
      841 ccagatctaa agcctctact actgccggct ggaaggtgcg cgcccccgaa ccttttcgtg
      901 tccaaatgcg tcctggaatt gcccaagaaa aatgacatgc ggaatttcat cgcgcaacac
      961 atgaaagaga tgggagatgg ggtatgatgc cagcggtgcg aaaatccccc caggaaagtt
     1021 cccccggtcc gttcggcacc gaggcctccg ccggtgtcaa cgaacccgtg acatgcttgg
     1081 gcaaagcctt ccccgacgac aacgcgcggc gggagcactt cctcggcctt ctgcgcgaga
     1141 agctccaaga cccggagttc cgaaagatcg aagggtttcc ccttggtgag gacgaggaca
     1201 tcctggcgct ctccgaccca ccgtactaca ccacctgtcc gaatccgtgg atcgccgact
     1261 ttatacggca ttatggcaag ccctatgacc cgaacgagcc ctatcgccgc gagcccttcg
     1321 ccgccgacgt gagcgaggga aaaaacgacc cgatctacaa cgcccattcc taccacacca
     1381 aggtgccgca caaggccatc atgcggtata tcctgcacta caccgaaccg ggggacgtgg
     1441 tcttcgatgg gttttgcggc tcagggatga cgggggtcgc cgcccagatg tgtggcgaca
     1501 aggcggtggt cgagtccctg ggttatcggg tggacgaaga tgggaccatt tcccggcggg
     1561 agaccgacga agaagggaag cccctctgga gggccttttc ccaggtgggg gcccgccggg
     1621 gagtgctggc cgacctgtcg cccgtggcca cgttcatcac ccataaccac accgccccgg
     1681 tggacgtcca cgaattcgaa cgcgaagccc ggcacgtcct cggggaagtg gaggccgaat
     1741 gcggctggat gtacgaaacc ctccacagcg atggaaagac caagggtcag atcaaccaca
     1801 ccgtctggtc ggatgtcttt atatgtccgg agtgcaccgg agagattgtc tttttcgaag
     1861 aagccgtcga ccgggatcag gggacggtga gggcggactt tccctgccca cactgccaag
     1921 ctcgccttac gaagaggagg atggatcgcg cccgggagac ccgattcgat ggggcattga
     1981 accaatccct cagccaaata aaacagcttc ccgtcttaat cgactatact gtggatggcc
     2041 gtagggccga gaaatacccc gacgacgccg acctggcgct catcgacaaa atagataacg
     2101 gcaacatccc ctactggttt ccggcggtcc gcatgccgga agggatagaa agccgtcgca
     2161 atgatcccct aggcatcacc catacacatc atttttattc aaaacgaaac ctatggattc
     2221 tatcgtcact ccggtcacgc tgcaccaccg ggccgatgtt gctgtggttt aattcccaac
     2281 tgatcaacgt cagtaaatta aaccgatatc gccccggggt atcctttccc tacaaccccc
     2341 tcagcggaac tctgtacatg ggatcccaaa cttccgaacc ttgcctattt actgcctaca
     2401 aaaacaaatt gaataaactt gtcaaatcac taaattccac caactcacct catatcatta
     2461 ccacgcaatc caccacacac atctcggaaa tgccctcaca gagtgtggat tatatatttc
     2521 tcgacccccc ctttggatca aacctgaact attccgagct tagcgcgctg tgggagcggt
     2581 ggatgaaggt ctggacgaac aacgccctcg aggccattga aaacgcctcc caggggaagg
     2641 accttgccgc ctatcgccgc ctcatggccg cctgcttcaa ggaagcccac cgggttctca
     2701 agccggggcg ctggatgacc gtggagttct ccaacaccaa ggcctccgtc tggaatagcc
     2761 tccaggccgc gctcacggag gcgggcttcg tcgtcgccga tgtctcggcc ttggacaagc
     2821 agcagggtag cttcaaggcg gttaccacgc ccaccgccgt caagcaagac ctcgttatct
     2881 ccgcctacaa gcccggtggg gggctgatgg agtccttctc cctggaggaa ggaccggaag
     2941 gaggggtgtg ggacttcgtc cgggctcacc tgggttattt gcccgtcgta aaaaagcacg
     3001 gcgacacctt gcaattcatt ccggagcgcg acccacgcat ccttttcgac cggatggtgg
     3061 cccacttcgt gcgcaaaggt catcccgtga cgctggacag cgccgagttt caggaggggt
     3121 tggctcggcg tttctcctac cgcgacggca tgtgttttct ccccgaccag atggccgatt
     3181 acgacagggg aaaaatgacc gccgggggca tggcgcccac gcctccctcg gtctccgacg
     3241 aatcctccgc catcgcatgg cttcgccaga tcctccagaa taggccgcag acctttcaag
     3301 atatccatcc tttattcatc aaggagctgg ggggatggca agggcgcgag aagcccctgg
     3361 agctctccga gctgctggga caaaactttc tgtgctacga cggcaaaggc gcggtgccga
     3421 gcccgattta cagctaccta tcgtcgacct tcgaggagct ccgggatcga cccaaagatg
     3481 acgaagatct ccgcgcccaa ggaaaggatc gatggtacgt ccccgacccg aacaaagcgg
     3541 gcgacttgga gaagctacgc gagaggtcgc tcctcaagga gttcgaggag tacaaagcct
     3601 ccgccacgag aaagctcaag gtcttccgca tggaggccgt ccgggccgga ttcaagaagg
     3661 cctggcagga acgggactac gccaccatca tcgccgtggc ccgcaaggtt cccgagaacg
     3721 ttcttcagga agacccccag ctcctcatgt ggtacgacca ggcgctgacg cgctccggag
     3781 gggaatgacg gccccacgcc cctccggcct gtcgcaatag atttccttgg ggaaaagaat
     3841 ccgggatagc gcactccccg acacgaccat gtgatcctcc attggggcat ggtgctggcc
     3901 gtctatccgt tctggggaga ggtggccctc cggaccagcc gactcctgag gctccagggc
     3961 tccgccgccg ccgcccatat ccagcgacgg atccgggagc agtacggtga gcgggagacc
     4021 gtctcccggg cggggcgccg cgtgctgcgg tcgtacgggg actggggcgt gctcaaaggg
     4081 agtggcgcca ggggagtcta cgccgcccga gccccctctc gccgtggaag atccccggct
     4141 cgtcgcctgg ctggtggaag cctccctccg cgcccggggc acggacgtgg ctccactgaa
     4201 ggggatcctg gagagcccgg gactttcccc cttttcggat caagccggtg cccgccgacg
     4261 gcctgggagc cgtgtcgtcc cggctcgatt ttctccgcca
//
LOCUS       DAOOER010000114         3849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_22245_length_3849_cov_3.729309, whole genome shotgun sequence.
ACCESSION   DAOOER010000114 DAOOER010000000
VERSION     DAOOER010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22245_length_3849_cov_3.729309"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1636
                     /locus_tag="PL162_09330"
     CDS             <1..1636
                     /locus_tag="PL162_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92259.1"
                     /translation="AKQPGNLSADFHQKFLTQCKVLGGIPSPVAAFSWGAGGNLPALG
                     KIFASFAREAVLDGMPSHTLYEKKYGIPLFDSTLGDVHDVLSFDTKAVQEITDAMVKS
                     WSIQFFRLDPPKILSGEPMPRLVLSFPAEWFDASSTAGRQIFLRMRSGDAALSLDTPP
                     GKDYAGVVSFDAARYLYVVDTGQTGSGYWYDYKPGMLVCLEAPRELTASLRNKTLEIV
                     WQEPRLAKEKPELVTGYRIQVRRLTEKLPLVDRPVPRGSTRLALGEADGVGSFENLEV
                     SARTVMTLGNRSGESEPAMALATPEASKIFPLFENRTYWTTATPLGNDPGVVFPQVPL
                     AVSVTLGGVTGSMGPEAPEWKAYLKLAENVAGTDAFLKKRFEAELASKGITLTEEQMR
                     QALQSMQVAGVFMPEGSSQKRALLGGGWRQPGAPVSVTLQVTLPSVPRIPLSLPGPLD
                     ILRPVECQVELSRWFMLVGDQKSPEFSSGRGTLSWTPKSASEDAFGIDLALPFSLTFF
                     DPATGDVLKEGGVPLKYENFTAIFPLGVFFCPEKKP"
     gene            1696..>3849
                     /locus_tag="PL162_09335"
     CDS             1696..>3849
                     /locus_tag="PL162_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92260.1"
                     /translation="MKKTGGMGRRALGVAILVGCLAVPGAWGEGRLFSVGPLGVEIPR
                     GWFPLPEGDGGWGFCTGGFPEGKEPFLSVMLRLVPNDQEFLEFRKQIDAARKDPQASV
                     DIVEEAGRVGPWEGTQMRFSVTMKGGERAGTSFQGRLFFSQGGVSGDHRLMVLVGGDP
                     TLAREREADVAQLLGSLREGGASYVLSPDLGLESSPGEFDGAAALAVDGEGLIYVANW
                     RQRTIRVLDAQGTIRGTWGKEGQGEPGTFAQIKTLLGLPEGGVLVGDGGYGYDAPVLH
                     ECDATGNFRRHVDLAGRAKPPMEYMLSLSRNDAGDFLVTDGDISEGNRLTVLNPEGTP
                     QRFWNVPKVTAAAWEPSGTVVASTFSEDRMGMLVRFAPDGTLQEAWTAFGRGTPPPGW
                     EAVYFDPKFLTVDPCGRIAVLDTWSEAVWVYEPDGTLRDRVDLKILARSGILEGIAAD
                     PQGHLLVLDRGSSGGVPPRVLVLRRTEPCEPPKVSPEREPDVLPSPLPDPTPTLSGEG
                     APELSPTGDLLTDRLEAWKKALELRRQAAELLREGRRSEALPLYEESLKLYEDAALRA
                     YLEDLRRGAPLATPAPASDDAEEMEDLPPLENVAEDLTGTPEPELPEPEISEDLAELP
                     ELPPVATLVPTRAPTVAPEPSPTPEPELPPLPPSPYEGEWITLMRAEGAPGGAPPGFA
                     LLKVRRQGDMLRCTLHDQQVFLLRDEGPERAEGSLG"
BASE COUNT          668 a         1259 c         1275 g          647 t
ORIGIN      
        1 ggcgaagcaa ccgggaaacc tttcggcgga ctttcatcaa aaatttctga cccagtgcaa
       61 ggtcctgggg ggaatcccct cgccggtggc ggccttctcc tggggcgcgg ggggaaatct
      121 ccccgccctg ggaaagatct ttgcctcctt tgcccgggag gcggtgctgg acggcatgcc
      181 gtcgcacacc ctctacgaaa agaagtacgg cattcccctc tttgacagca ccctggggga
      241 tgtccacgac gtgctttcct tcgacaccaa ggcggtgcag gagatcaccg acgccatggt
      301 gaaatcctgg agcatccagt ttttccgtct cgatcctcca aaaattcttt cgggggagcc
      361 catgcctcgg ctggtcctct cctttcccgc agagtggttc gacgcctcct ccaccgccgg
      421 acgtcagatt ttcctgcgga tgcggtcggg agacgcggcg ctctcgctgg acactccgcc
      481 gggcaaggac tacgccggag tggtgtcctt cgacgcggcg cgctatctct acgtggtgga
      541 cacggggcag accggttcgg ggtattggta cgactacaag cccgggatgc tggtgtgcct
      601 tgaggccccc cgggagctca ccgcctcgct ccggaacaag accctggaaa ttgtctggca
      661 ggaaccccgg ctggccaagg aaaagcccga actggtgacg ggataccgca tccaggtgcg
      721 gcgccttacg gaaaaactcc ccctggtgga tcgcccggtg ccccggggaa gcacccgtct
      781 ggcactggga gaagccgacg gggtgggaag tttcgagaat ctggaggtct cggcccgcac
      841 ggtgatgacc ctgggaaatc gcagcggcga gagcgaaccc gccatggccc tggccacacc
      901 ggaagcctcc aagatctttc ccctttttga gaaccgcacg tactggacca ccgccacgcc
      961 tttggggaac gaccccggcg tggtctttcc ccaggttcct ctggcggtgt cggtgaccct
     1021 ggggggggtg acggggtcca tgggccccga ggcgccggag tggaaggcct atctgaagct
     1081 cgccgaaaac gtggcgggaa cggacgcctt tctcaagaag aggtttgaag ccgagcttgc
     1141 ctccaagggc atcaccctca cggaggagca gatgcgccag gcccttcaat ccatgcaggt
     1201 ggcgggggtc ttcatgcccg aaggctcgtc ccagaaacgg gccctcctgg gaggaggatg
     1261 gcgccaaccc ggcgcgccgg tctccgtgac gctccaggtg acccttccct cggtgccccg
     1321 tattcccctg tcgttgccgg gacctctgga catcctccgc cctgtggagt gccaggtgga
     1381 gctttcccga tggttcatgc tggtgggaga tcaaaaaagc ccggaatttt cctcgggaag
     1441 gggaacgctt tcctggaccc ccaagagcgc ttccgaagac gccttcggta tcgatctcgc
     1501 cctgccgttc tcgctgacct ttttcgaccc cgccacgggg gacgttctca aagagggagg
     1561 agttcccctg aagtacgaaa acttcaccgc catctttcct ctaggggtgt ttttctgccc
     1621 cgagaaaaaa ccctgaccat gggcattatc gcgtcaagac cggaacgacc gcaggaaaaa
     1681 acgggaggga tccgcatgaa aaaaacgggt ggcatggggc ggcgagccct gggagtggca
     1741 atcctggtgg gatgcctggc agtgccggga gcctggggag agggacggct cttctccgtg
     1801 ggccccctgg gggtagaaat ccctcgggga tggtttcctc tccccgaggg ggatggggga
     1861 tggggctttt gcacgggggg atttcccgaa gggaaggagc cctttctctc cgtcatgctc
     1921 cgcctggtgc ccaacgacca ggagtttcta gagttccgga agcagatcga cgccgcccgc
     1981 aaagatcccc aggcctcggt ggacatcgtc gaggaagcgg ggcgcgtggg accctgggag
     2041 ggcacccaga tgcgcttttc cgtgaccatg aaggggggag agcgcgcggg gacctctttt
     2101 cagggaagat tgttcttctc ccagggcggc gtgagcggag atcatcggct gatggttttg
     2161 gtgggaggag accccaccct tgcccgggaa cgggaggccg acgtggcgca gctcctcgga
     2221 tccctccggg aagggggggc gtcctacgtc ctctcccccg atctggggct tgagagctcg
     2281 ccgggggagt tcgacggcgc tgcggcgctg gcggtggacg gcgaagggct catctacgtc
     2341 gccaactggc gccaaaggac catccgggtg ctggacgccc agggcacgat ccggggcacc
     2401 tggggcaagg aaggccaggg agagccgggg accttcgccc agatcaaaac gttgctggga
     2461 ctcccggaag ggggcgtgct ggtgggcgac ggaggctacg gctacgacgc ccccgtgcta
     2521 catgagtgcg atgcgacggg gaacttccgg cggcacgtcg acctggcggg ccgcgccaaa
     2581 ccccccatgg aatacatgct ttctctgtcc cgaaacgacg caggagattt tctcgtcacc
     2641 gacggcgaca tcagcgaagg aaaccgcctc acggtcctca accccgaggg cactccccag
     2701 cgcttctgga acgtccccaa agtgacggcg gcggcctggg aaccttcggg caccgtggtg
     2761 gcttccacct tctcggaaga ccgcatgggc atgctggtcc gcttcgcccc cgacggcacg
     2821 ctccaggaag cctggaccgc cttcggccgg ggcaccccac ccccgggatg ggaggcggtg
     2881 tacttcgacc ccaagttcct caccgtggac ccctgcgggc gcatcgccgt tctggacacc
     2941 tggagcgaag cggtgtgggt ctacgaaccc gacggcaccc tgcgggaccg ggtggacctc
     3001 aaaattctgg ctcgatcggg gattctggaa ggcatcgccg cagaccccca gggccatttg
     3061 ctggtcctcg accgaggaag ttccggtgga gtgccccccc gggtgctggt gctccggcgg
     3121 accgagccct gcgaaccccc gaaggtgtcg ccggaacgtg agccggacgt cctcccctct
     3181 cccctccccg accccactcc caccctgtcc ggggaagggg cgccggagct ctctcccacc
     3241 ggcgatctgc tcaccgatcg tcttgaggcc tggaaaaaag ccctggagtt gcggcgccaa
     3301 gcggcagagc tcctgcggga agggcgccgg agcgaggccc tgcccctcta cgaggagagt
     3361 ctgaagctct atgaggacgc cgccctgcgg gcctatctgg aagatttgcg gcggggcgcc
     3421 cccctggcga ccccggcccc ggcctccgac gacgccgagg agatggaaga ccttcctccc
     3481 ctggagaatg tggccgaaga tctcaccgga acccccgaac ccgagctccc cgagcccgaa
     3541 atttcggagg atctggcgga gctccccgag cttccccccg tggccaccct ggtgcccacc
     3601 agagcaccga cggtggcgcc ggagccttcg cccacgccgg aacccgagct accccccctc
     3661 cccccgtctc cctacgaagg agaatggatc accctcatgc gggccgaagg ggccccggga
     3721 ggggctcccc cgggatttgc cctcctgaag gtgcggcgcc agggggacat gctccggtgc
     3781 accctgcacg accagcaggt ctttctgctg cgggacgagg ggccggagcg cgccgagggc
     3841 tccctgggg
//
LOCUS       DAOOER010000115         3822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_22465_length_3822_cov_8.573401, whole genome shotgun sequence.
ACCESSION   DAOOER010000115 DAOOER010000000
VERSION     DAOOER010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22465_length_3822_cov_8.573401"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..1249)
                     /locus_tag="PL162_09340"
     CDS             complement(128..1249)
                     /locus_tag="PL162_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQF92261.1"
                     /translation="MDRCGPFSRPLPLPVAGGAGPLGVSIIFRILGGLLLAGGVLLWG
                     SAGGAEPRKVILTLHWYPQTQFAGYYMAQEKGFYLRRGIDMEIRRGGADVDGPRELQE
                     GRTDFVTMFLTGALRFRTADLPLVNVGQMVGRGNTLIVAKKKSGILAVADLDKRRMSL
                     WGDVFSGAYKQLFAARKIWPVVVPQFFSVELFLRGAVDACCAMTYNEYLRILQAGYRP
                     DELTVISLRDEGCNVPEDGIYCLAPTWKKDPELCRNVREASLEGWRYAAAYPEEAVAL
                     IQKKADEAGYTVNRLLLWHMLQEIIPSIFPDKEGTWVPGRLSRQDYERTAAMMHQVFV
                     LPGDVVPYEEFVPFGEGSVEENALESADMPEKGTPSYVP"
     gene            complement(1283..1681)
                     /locus_tag="PL162_09345"
     CDS             complement(1283..1681)
                     /locus_tag="PL162_09345"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQF92262.1"
                     /translation="MERTWSATLDHMEDMRQFILGAAEGKLSPRREAHLDLAVEEIVV
                     NICSYAYEIPPGEITVRVEETEDSLGVEFIDNGVPFDPLAAEEPDVTRPLADRDQGGL
                     GILLVRRVMDEVHYRREGGRNCLRIAVKKG"
     gene            complement(1692..2027)
                     /locus_tag="PL162_09350"
     CDS             complement(1692..2027)
                     /locus_tag="PL162_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HQF92263.1"
                     /translation="MDIHAESVPQGRVVRLVGRLDTKTAPDFEKEALMWVEEGCRGVA
                     LDLSGIDYVSSAGLRSILVLAKKLQSVEGRVVLFGMSGVVEEVFSISGFDRLIPVVED
                     RDAACKTLG"
     gene            complement(2113..2925)
                     /locus_tag="PL162_09355"
     CDS             complement(2113..2925)
                     /locus_tag="PL162_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQF92264.1"
                     /translation="MLDLQGLVKTFHPGTENEVRALQGVDLRVEEGSFVVVIGTNGSG
                     KSTLQNAISGSFFVDRGRIELAGQDITRWPEHRRAALIGRVFQNPFSGTAPSMSIGEN
                     LALAMRRGERRTLGWNAFFRRSEELTERVRRLGMGLEDRLRNEIGSLSGGQRQALTLL
                     MATLRRPELLLLDEHTAALDPRAADLVIRLTDELVREGRLTTVMVTHSMPQAVSLGDR
                     LVMMHRGRVVADLRGEEKRRLRPGDLLDRFDEIRRREQIDPSVAEMLRETYV"
     gene            complement(2918..>3822)
                     /locus_tag="PL162_09360"
     CDS             complement(2918..>3822)
                     /locus_tag="PL162_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQF92265.1"
                     /translation="GLLLALMALGIFVTFRLFRFADLATDSILTLGAAVTAVLLVRGA
                     SPGGATLAGAGAGILAGAVTGLLHTRLHINSLLSGILVMTGLYSVNLRVMGGSNVPLA
                     HVPTGADRARDALQYISGVEVWSFSSWEIPGGDLAVLLGILGVVALFGFGLYAFLLTD
                     FGTALRASGDNGQMALAQGVSAPHMMLVALMISGGMSGLTGALLAQYQGFADIQMGVG
                     TLVLGLASVIMGQALVRSSSLGISIAGAVMGSVLFRLLVSIALRWGLNPNDLKLATAS
                     FVLITLVLPKLMGSWSNRKEAPHA"
BASE COUNT          746 a         1348 c         1080 g          648 t
ORIGIN      
        1 gcgggaccgg aactcctgct ccacggcgcg cagcgaagaa aaataaccgt atcccacaat
       61 agcgagaaag aggaaccccg cccctcccat ggtcatgagc gccagttttc ccgcaatacc
      121 cagatgtcta gggaacatag gaaggagtcc ccttttcggg catatctgcg ctctccaagg
      181 cgttttcttc gacggatccc tcgccgaaag gcacaaattc ctcgtaggga accacgtctc
      241 cgggaagaac gaagacctgg tgcatcatgg cggcggtgcg ctcgtaatcc tggcgggaaa
      301 gacgtccggg cacccacgtc ccctccttgt cgggaaatat ggagggaata atttcctgca
      361 acatgtgcca caggagcagc cgattgaccg tgtatcccgc ctcgtcggcc tttttttgaa
      421 tcaacgccac cgcttcttcc gggtaggcgg cggcgtagcg ccacccctcc agcgaagctt
      481 cccgcacgtt ccggcaaagc tccggatcct tcttccatgt gggcgcaagg cagtagatgc
      541 cgtcttcggg gacgttgcac ccctcatccc gcagggagat cacggtaagc tcgtcggggc
      601 ggtatcccgc ctgcagaatg cggaggtact cgttatacgt catggcgcaa caggcatcca
      661 ccgctccgcg caggaagagc tccacggaaa agaactgggg caccaccacg ggccagatct
      721 tccgggcggc aaaaagctgc ttgtaggccc cggaaaagac gtctccccag aggctcatgc
      781 ggcgcttgtc cagatctgcc acggcaagga tgccgctctt ttttttggcc acgatgaggg
      841 tattgccccg ccccaccatc tgccccacgt tcaccagggg caggtccgcc gtgcgaaagc
      901 gcagcgcccc ggtgagaaac atggtgacga agtccgtgcg cccctcctgg agctcccggg
      961 ggccgtccac atcggcgcct ccccggcgaa tttccatgtc aatcccccga cgaaggtaaa
     1021 aacccttctc ctgggccata tagtaccccg caaactgggt ctgggggtac cagtgaagcg
     1081 tcagaatcac cttccggggt tcggcgcccc cggcgctccc ccaaaggagc accccccccg
     1141 cgagcagaag ccctcccagg atcctaaaga ttatcgaaac ccctaaaggc ccggctcccc
     1201 cggccaccgg cagaggaagg gggcgggaga aagggccgca cctgtccatg gcttttccct
     1261 cccacttaga tcggtccttg acctagccct tcttcaccgc gatcctcaga cagtttcgcc
     1321 ccccttccct ccggtagtgg acctcgtcca tgacgcggcg caccaggagg atgcccaacc
     1381 ccccttgatc ccggtcggcc agggggcgcg tcacgtcggg ttcttctgcg gcgagggggt
     1441 cgaagggcac gccgttatcg atgaactcca cccccaggga gtcttccgtt tcctcgacgc
     1501 gcacggtgat ctcccccggg ggaatctcat aggcatagga gcagatattt accacgatct
     1561 cctccaccgc caggtccagg tgggcctccc gtcggggcga aagcttcccc tcggcggccc
     1621 ccagaataaa ttgacgcatg tcctccatgt ggtccagggt ggccgaccag gtccgctcca
     1681 tggtcccact cctagcccag ggttttacaa gcggcatccc gatcttctac cacaggaatg
     1741 agccggtcga agcccgagat ggaaaagacc tcttccacca ccccggacat gccaaaaagg
     1801 accacccgcc cctccacgga ctgaagtttt ttcgccagca cgaggatgga acgcagcccc
     1861 gcgctggaga cgtaatcgat acccgacaga tccagggcca cccctcggca cccctcttcc
     1921 acccacatca gggcctcctt ttcgaaatcc ggggcggtct tggtgtccag tcgccccacc
     1981 aggcgcacca cacgcccctg gggaacgctt tctgcgtgaa tatccatcgc ctctccctcc
     2041 cttatctccc ttatctcctt tatctccctc cgaaagccct cgtccccggt ctttcccctg
     2101 gacaaaaggc ccttacacat aggtctctcg caacatctcc gccaccgagg ggtcgatctg
     2161 ttctcggcgc cttatctcat cgaatcggtc caacaggtct cctgggcgca gccgcctttt
     2221 ctcctctccc cgcaggtcgg ccaccacgcg cccccggtgc atcatgacga ggcgatctcc
     2281 caggctcacc gcctggggca tggaatgcgt caccatcacg gtggtcaggc gcccctcccg
     2341 caccaactcg tcggtgaggc gaatcaccag gtccgccgcc cgggggtcca gcgccgccgt
     2401 gtgctcgtcc agcagcagga gttccgggcg ccggagggtg gccatcaaaa gcgtgagggc
     2461 ctggcgttgc ccccccgaca gggagccgat ctcgtttctc aggcggtctt ccagccccat
     2521 gcccaggcgc cggacccgct ccgtcaactc ttccgaccgg cggaaaaaag cgttccatcc
     2581 cagggtgcgc cgctcccccc ggcgcatcgc cagggccagg ttttcaccta tggacatgga
     2641 aggggcggtg ccgctaaagg ggttttggaa tacccgaccg atgagggcgg cccgccggtg
     2701 ctccggccac cgggtgatat cctgccccgc cagctctatg cgcccccggt ccacgaaaaa
     2761 gctccccgag atggcgtttt gcagggtaga tttccccgag ccgttggtgc ctatgaccac
     2821 cacgaaggag ccctcttcca ccctcaggtc caccccctgg agggccctta cttcgttttc
     2881 cgtcccgggg tgaaaggtct tgaccagtcc ttgaagatca agcatggggt gcctctttcc
     2941 tgttgctcca agaccccatc agcttgggca acaccaacgt gatcagcaca aaggacgccg
     3001 tcgccagctt gaggtcgttg ggattgagcc cccatcggag ggcgatggag accagaaggc
     3061 gaaagagcac cgaccccatc accgcccccg cgatggagat ccccaaagac gagctccgga
     3121 ccagggcctg ccccatgatg acgctggcca gacccaacac cagggtcccc actcccatct
     3181 ggatgtcggc aaagccctgg tactgggcca agagtgcccc ggtgagaccc gacatccccc
     3241 ccgagatcat gagggccacg agcatcatgt ggggggcgct cactccctgg gctagggcca
     3301 tctggccgtt gtctccggag gcccgcaggg ccgtaccaaa gtccgtgagg agaaaggcat
     3361 acaggccaaa gccgaaaagg gccaccactc ccaggatccc caggagcacg gccagatcgc
     3421 ccccggggat ctcccacgag gaaaaagacc aaacctccac tccggagatg tactggaggg
     3481 cgtcccgggc ccgatccgcc cccgtgggga cgtgggccag gggaacgttg ctccctccca
     3541 tgacccgcag gttcaccgaa tagagccccg tcatgaccaa aatgcccgat agtaggctgt
     3601 tgatgtgcag tcgggtgtga agaaggcccg tgacggctcc cgccaggatt cccgcccccg
     3661 cccccgccag ggtggcccct ccgggggagg ccccccggac caggagcacc gccgtcaccg
     3721 ccgcccccag cgtgaggatg ctgtccgtgg ccaagtcggc aaagcgaaaa aggcgaaagg
     3781 tcacgaagat ccccagggcc atgagggcca gaaggagccc ca
//
LOCUS       DAOOER010000116         3758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_22938_length_3758_cov_8.215771, whole genome shotgun sequence.
ACCESSION   DAOOER010000116 DAOOER010000000
VERSION     DAOOER010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 3758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3758
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22938_length_3758_cov_8.215771"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..674)
                     /gene="purE"
                     /locus_tag="PL162_09365"
     CDS             complement(111..674)
                     /gene="purE"
                     /locus_tag="PL162_09365"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HQF92266.1"
                     /translation="MHFRRDIGARARTARPRVGVLLGSASDLPLAQEAAEIFRALDIP
                     FEVTVASAHRTPDHVAAYAASAQKRGLRVLLAVAGLSAALPGVVAAHTLLPVLGVPVA
                     SGSLGGLDALLAVTQMPPGVPVGSLGVGGGKNAALLAARILALHQPGLGARLQAWHQE
                     ASDKVLASRKKMGDLPQPPAEAFFSAK"
     gene            complement(654..1580)
                     /gene="purD"
                     /locus_tag="PL162_09370"
                     /pseudo
     CDS             complement(654..1580)
                     /gene="purD"
                     /locus_tag="PL162_09370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
     assembly_gap    1584..1683
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1684..2831)
                     /locus_tag="PL162_09375"
     CDS             complement(<1684..2831)
                     /locus_tag="PL162_09375"
                     /inference="COORDINATES: protein motif:HMM:NF013931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase PurH"
                     /protein_id="HQF92267.1"
                     /translation="MTKRRALLSVWSKEGLVPFARELQKLGWDLVSSSGTAKALVDGG
                     LTVTEVAELTGLPPMLGGRVKTLHPAIAGGILARRHVPVDREDVERFSIPLLDMVVCT
                     LYPFEEKALQGEPLEVLLEHIDIGGVTLLRAAAKNFPHVVVVTDPGDYSLVLEELRSQ
                     GDVTLPTRQRLALRAFSATALYDATIEEGLRSAMGLPRPGPEAPLLVRPMHRAQELRY
                     GENPHQLAGLFLSPLERAPWKILGGKSLSYNNILDLDTALRGMELFSDAPSCILVKHC
                     TPCGIARGSSGLEALERAWACDPVSAYGGIAGFSREVDAKTCEELGEHFLEIVAAPSF
                     DPAGLEKLKNRRPSLRLVQWPGRHLDSLEYRSTWSGTLVQEDRLPPIPS"
     gene            complement(2794..3429)
                     /gene="purN"
                     /locus_tag="PL162_09380"
     CDS             complement(2794..3429)
                     /gene="purN"
                     /locus_tag="PL162_09380"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HQF92268.1"
                     /translation="MTPRMAILISGRGSNMEALARRCIAGDLAADIAFVGSDRERAPG
                     LEIARSLGLATAVFPYEELGRPRAEGQLEETLRSEAVSWVVLAGFMRVLSAPLVSAFA
                     NSIVNIHPSLLPAFPGMRSIREAYDHGVRVTGVTVHLVDRQVDHGPILAQEPVRVHDG
                     DTLGTLEERIHAAEHDLYWRTLQDLFAGVFKLDGRRRMVRDQAPSFAVRME"
     gene            complement(3426..>3758)
                     /locus_tag="PL162_09385"
     CDS             complement(3426..>3758)
                     /locus_tag="PL162_09385"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HQF92269.1"
                     /translation="PTRLYVSSALQACATGKVKGMAHITGGGLEENIQRILGGYRPAL
                     NFGSWHRPPIFDVLQRAGDITEDEMRRVFNLGIGFVFVVAPEDIRGVTAMLQDLGESP
                     VVIGDVIS"
BASE COUNT          664 a         1126 c         1225 g          643 t
ORIGIN      
        1 ttgtccaaga tgaggtcttc gggattttcg tcgtcgctgg ggataggggg agaaaaggga
       61 agcccccgga cccaggggtc agggggcgcg atctctggtg cggaggcgtc ctacttcgcc
      121 gagaagaagg cctcggcggg gggttgggga agatctccca tcttttttcg agaggcgagg
      181 accttgtctg aggcctcttg gtgccaggcc tgaagccggg cgcctaaccc cggctggtgt
      241 agcgccagga tgcgggcggc caggagggcg gcatttttcc ctcccccgac gcccagagat
      301 cccacgggga ctccgggggg catctgggtc acggccagga gggcgtctag ccctccgagg
      361 gaccccgatg ccaccggcac gccgaggacg gggaggagag tgtgggcggc caccaccccg
      421 gggagggcgg cggaaaggcc cgccacagcc aggagcaccc ggaggcccct cttttgggcc
      481 gaggccgcgt aggcggcgac gtggtccggg gtgcggtggg ccgaggccac cgtgacttca
      541 aagggaatgt ccagggcccg gaagatctct gccgcctcct gggccagagg caagtcggag
      601 gcggagccga gcaggacgcc cacccggggt cgggcggtac gagcccgagc cccgatgtcc
      661 cggcggaagt gcatgccggg aaaagagatc ttttttaccc cttcataggc attttcccaa
      721 gcttcttcta ggtcgtttcc ccgccccacc accgtgagca ctcgccctcc ggcggtgatg
      781 acctcgtctg aggagttctt tatcgttccc gcatggaaga ccatcacgtc gtcccggcct
      841 gcaagggctt cgagcccgga aatgacgcgg cccttttcgt aggggcccgg gtacccttcg
      901 gaggcgagga ccactcccac cgtggctcgg ggagactccg agggccacgg gtacgaggcg
      961 aggtctccct gagcacaagc taaaagggcc tcggcccagt ccatatcaag ggccgggagg
     1021 accacctggg cctcggggtc tccgaagcgc acgttgtact ccaccacgtg gggttcgcct
     1081 cccaccacca taaggcctac gtaaagaacg ccgcagaagg gaatgccctc ttcctggaga
     1141 ccccgagcgg tgggctccag gatacgactt tctattttct gcatgaggcg agcgtcggct
     1201 tggggtacgg gggtgtaggc tcccatgcct ccggtgttgg gcccttggtc cccttcggcg
     1261 acgcgtttgt ggtcctggct tggagggagg aggcgaaagc tttgcccatc gaaaagggcc
     1321 aggaccgtca tctcccatcc gggcaaaaag tcttccacca ccacggtttt ccccgcttct
     1381 cccaggacgt gcttttccag gagatcttgg cacaggcgat cggcttcttc tcgggtctcg
     1441 ggcaaaaagg cccctttgcc cgccgccagg ccgtcggctt tgaccacgaa gggaggagtt
     1501 ctctggtcca gggcccgctg ggcttctgcc agggattgac acacggcgaa gggggcggtg
     1561 ggaatgccat ggcgccccat gaannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnngagggga tgggaggaag gcgatcttcc tggaccaggg tgccgctcca ggtggaacga
     1741 tactccaagg aatcgaggtg tctcccgggc cactggacaa ggcgcaggga ggggcggcga
     1801 ttttttagtt tttccaaccc tgcggggtca aaggacggag cggccacgat ctcgagaaaa
     1861 tgctccccga gctcctcaca ggtcttggcg tccacctccc gggaaaaccc cgcgatccct
     1921 ccataggcag aaacgggatc acaggcccag gcgcgctcca gagcttcgag gccgctggat
     1981 cctcgggcga tgccacaggg ggtgcaatgc ttcaccagga tacacgacgg ggcgtcggaa
     2041 aagagctcca tgcccctgag ggccgtgtcc aggtctagga tgttgttgta ggagagggat
     2101 tttcccccca ggattttcca gggggccctc tccaggggag aaaggaaaag ccccgcaagt
     2161 tggtgggggt tttccccata tcggagctct tgggcccggt gcatgggacg caccaaaaga
     2221 ggggcctcgg ggccgggccg ggggaggccc atcgcggagc gcagcccctc ttcgatggtg
     2281 gcgtcgtaga gagccgtggc agagaaggcc cgaagggcca ggcgttgacg agtggggagg
     2341 gtaacgtcgc cctgggaacg aagctcttcc aggacaaggg agtagtcccc agggtcggtt
     2401 actaccacga cgtgagggaa attcttggcg gcggcccgca agagggtcac cccgccgatg
     2461 tcgatatgct cgaggaggac ttccaggggc tccccttgaa gggctttttc ttcgaagggg
     2521 taaagggtac agaccaccat gtccagaagg ggaatggaaa agcgttccac atcctctctg
     2581 tcgacgggga cgtggcgacg ggccaggatt cctcccgcta tggcggggtg aagggttttt
     2641 acccgccccc ccagcatggg ggggagtccc gtgagctctg ccacctccgt cacggtgagg
     2701 cccccatcga cgagggcctt agcggtgccg gagctcgaca ccaggtccca ccctaacttc
     2761 tgcagttctc gggcaaaggg tactagccct tctttactcc atacggacag caaagctcgg
     2821 cgcttggtca cgaaccatac gcctccttcc gtcgagtttg aagactccgg cgaagaggtc
     2881 ctgcagcgtc ctccagtaca ggtcatgttc cgcagcgtgg atgcgctctt ccagtgtccc
     2941 cagggtatct ccgtcgtgca cccgtaccgg ctcttgggcc aggatggggc cgtggtccac
     3001 ctggcgatcc accaagtgga cggtgacccc cgtcacccgg acgccgtggt cataggcttc
     3061 tcgaatggac ctcatgccgg gaaaggcggg caagagggag gggtggatat tgacgatgga
     3121 gttggcaaag gcgctcacta agggggcgga gaggacgcgc atgaaacccg ccagcacgac
     3181 ccacgacacg gcctccgacc tcagggtctc ttccagctga ccttctgccc ggggcctccc
     3241 gagctcttca taggggaata ccgccgtggc tagacccagg ctgcgggcga tctccaggcc
     3301 gggggcccgt tcccggtccg atcccacaaa ggcgatgtcc gccgcgaggt ctccggcgat
     3361 gcacctccga gccagggcct ccatgttgga cccccggcca gagatgagga tcgccatgcg
     3421 gggcgtcatg agatcacgtc cccaattacc accgggctct ctcccagatc ctggagcatg
     3481 gcggtgacgc ccctgatgtc ctccggggcc acgacaaaga cgaaacctat ccccaggtta
     3541 aagacgcgtc tcatctcgtc ttcggtgatg tctcccgctc gctgtagcac gtcaaagatg
     3601 gggggcctgt gccaggagcc gaaattcagc gcggggcgat atcctcccaa gatccgctgg
     3661 atattttcct ctagcccccc tccggtgatg tgggccatgc ccttgacctt tcccgtggca
     3721 caggcttgga gcgccgagga gacgtagagc ctggtggg
//
LOCUS       DAOOER010000117         3684 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_23575_length_3684_cov_7.224580, whole genome shotgun sequence.
ACCESSION   DAOOER010000117 DAOOER010000000
VERSION     DAOOER010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23575_length_3684_cov_7.224580"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1426
                     /locus_tag="PL162_09390"
     CDS             <1..1426
                     /locus_tag="PL162_09390"
                     /inference="COORDINATES: protein motif:HMM:NF019151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HQF92270.1"
                     /translation="YEKYWRPMEVDFGQEAYSAHFDAFMRHYLTVKSGEIPKQGEVYE
                     AFKFYARSGDIVSQGMDRLAEDLRLYARFFCAMALDAEKDPDLQRAFFDLRTLKVDVA
                     YPFLLVMYARYHEGLLPKKDFLELIRLVESYVFRRAICEIPTNSLNKTFATFAASAKE
                     LDPDRLLEGLKAHFLLLPSYRRFPSDDEFRRDLRTRDLYHFRSRSYWLRRMENHERKE
                     HVSTEEYTVEHIMPQNENLSAAWQTDLGPEWKRIWEDWLHTLGNLTLTGYNSEYSDRP
                     FREKRDMNGGFKESPLKLNKGLGALEKWDENAIKQRAENLSAMALAIWKAPALPKETL
                     DSHRTRTPRPSSYTIDNHPYLSDKDGKFSAKTRVLFEMLRKEILALDPCVTEEFRKLY
                     VAYKAETNFVDVVPQAKRMRLSLNMPFSDIVDPRSLCRDVKGVGRWGNGDVEVFLSSE
                     DEIPYIMSLIRQSFERQMDNGEGE"
     gene            complement(1705..1965)
                     /locus_tag="PL162_09395"
     CDS             complement(1705..1965)
                     /locus_tag="PL162_09395"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HQF92271.1"
                     /translation="MPDRYDVQIKSGAARELKSLPRDVAELIARVLLSLEENPRPRGC
                     LKLTGSDAYRLRLGDYRIVYEIFDKTLVVLVIRIAHRRDAYR"
     gene            complement(1958..2176)
                     /locus_tag="PL162_09400"
     CDS             complement(1958..2176)
                     /locus_tag="PL162_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92272.1"
                     /translation="MPTKLKDPGPRRAVYRIIRDLPPEDVEKVASYAAFLAQQRRLED
                     EEDLHVARERRSQDSLPLRKVLQEMGIA"
     gene            complement(2506..2958)
                     /locus_tag="PL162_09405"
     CDS             complement(2506..2958)
                     /locus_tag="PL162_09405"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HQF92273.1"
                     /translation="MEKLPGLTISSSEAYSRLEKLAPLGREIGWSHNLAILERCKDPL
                     EREFYLRMTRKFGWSKNVLIHQIDNQSYEKSLLGQTNFDRALTPELRAQAMGSTPFPP
                     LRCLDSTKGVTGIRPGFMAPLHFWGFRPGKPGDFCLATLPTSILQQTA"
     gene            complement(3351..>3684)
                     /locus_tag="PL162_09410"
     CDS             complement(3351..>3684)
                     /locus_tag="PL162_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92274.1"
                     /translation="QVFLLRDEGPERAEGSLGGLTLRLEPGSEDDTRTVTLLQGDRVL
                     GTLFVIRAQAGAEAPPGETLELRALRLAFRQALEAYRQALARQSDDVEPLRKKVVLTW
                     LEYGSRRP"
BASE COUNT          762 a         1088 c         1132 g          702 t
ORIGIN      
        1 gtacgaaaag tactggcgac cgatggaggt cgacttcggc caggaggcgt acagcgctca
       61 ctttgacgcc tttatgcgcc actatctcac cgttaaaagc ggagaaatcc cgaaacaggg
      121 agaagtttac gaagccttca aattctacgc gcgctccggc gatatcgtct cgcagggaat
      181 ggatcgcctt gccgaagacc ttcgccttta cgcccgattc ttctgcgcca tggccctcga
      241 cgccgagaaa gaccccgatc tgcagagggc gtttttcgac ctcaggacgc tcaaggtcga
      301 cgtggcctac ccctttttgc tggtgatgta tgcccgatat catgaagggc tgttgccgaa
      361 aaaagacttc ttggagctga tccgtcttgt ggaatcctac gtcttccgcc gcgccatctg
      421 tgaaatcccc acaaactcgc tcaacaagac cttcgccacc ttcgccgcct ctgcgaaaga
      481 gcttgatccg gatcgccttc ttgagggcct gaaagcgcac tttcttctgc tcccctccta
      541 tcggcgcttc cccagcgacg atgagttccg ccgcgacctc cggacgcgcg atctatacca
      601 cttccgcagc cgcagctact ggctgagaag gatggagaac cacgaacgaa aggaacacgt
      661 gagcacagag gagtacaccg tggaacacat catgccgcag aacgagaacc tttccgccgc
      721 gtggcaaacc gatctgggcc ccgagtggaa acggatctgg gaggattggc tgcacaccct
      781 gggaaacctg acgctcaccg ggtacaactc ggagtacagc gaccgcccgt tccgggagaa
      841 acgcgacatg aacggaggct tcaaggagag cccgttgaag ctcaacaagg ggctcggggc
      901 cctcgaaaag tgggacgaga acgccataaa gcagagggcc gaaaatctga gtgccatggc
      961 gctggccatc tggaaggccc ccgccctgcc gaaggagacg ctggactccc atcgcaccag
     1021 aaccccccgc ccatcttcct acaccatcga caatcatccc tatctttccg ataaggacgg
     1081 gaaattcagt gctaaaacgc gcgtcctgtt cgagatgctg cggaaggaga tcctcgccct
     1141 cgacccctgc gtcaccgagg agttccggaa gctctacgtc gcgtacaagg cggagaccaa
     1201 tttcgtggac gtcgtccctc aggctaaacg gatgcgcctt tctctgaaca tgcccttttc
     1261 cgacatcgtc gacccccgtt cgctctgcag ggacgtgaaa ggagtgggcc gatgggggaa
     1321 cggcgacgtc gaggtcttcc tctcctcgga ggatgagatc ccctacatca tgagcctgat
     1381 ccgccagtcc ttcgagcggc agatggacaa tggggaaggg gagtgaggcc cctccctttc
     1441 aaggacagaa tttttgtctg attagcttta ccgcccctcg gaggcggccg cgcaccgagg
     1501 agcccgtccg tgcgacagat gccatgcgcc accgtcgacg gacgccaagc gacggggcgg
     1561 ttgacaggta ctgggtacgc cgataggtaa caacgaacct tgacaaccgg cacgggaatt
     1621 tcgaacgggc acgccggagg gcgcgaaact ggcgggactg attcggaagg ctggcggggt
     1681 gaaccttgaa aactctttgc gggatcagcg gtaggcgtcg cgacggtggg cgatgcggat
     1741 gacgaggacc acaagggtct tgtcgaagat ctcgtagacg atgcggtagt cgccaagacg
     1801 gagacggtag gcgtcgcttc cggtgagctt gaggcacccc cgaggacggg ggttttcttc
     1861 aagagaaagg agcacccggg cgatgagttc cgccacgtcg cggggaaggc ttttgagttc
     1921 tctggcggcg ccggatttta tttgaacgtc gtagcggtca ggcaataccc atctcctgga
     1981 gaactttccg gagaggcagg gaatcctggg atcggcgctc tctggcgacg tggaggtctt
     2041 cttcgtcctc aaggcgccgc tgctgtgcca ggaaggcagc atagctggcc accttttcga
     2101 cgtcctcggg aggaaggtcg cggatgatgc ggtacacggc gcgacggggt ccaggatctt
     2161 tgagttttgt gggcacgaga aacactcctt tcgcggggca tgcggcattc cttccacaaa
     2221 gaaaaacccc ccgaaggact gaattgccgg aggggctgcc tccgtcctcc ggggggagta
     2281 aacgaacgat atgaaacaac cagattgtac aggattcacg gagggtgtca atctcgtccc
     2341 ttcagcctag atccgcacct tgagaatacg caaaacctct ctggcctgcg cggagaacag
     2401 tgctaaggtg gagtttgcaa agccctgtgt ttgtcaacag aaaacacccc caggctgctc
     2461 ctcaaaaatc cccccacccc tgttcccctc gccctatgga tgggtctacg ctgtttgttg
     2521 caaaatagaa gtgggcaaag ttgcaaggca aaagtcccca ggtttgccgg ggcgaaatcc
     2581 ccagaaatgc aaaggggcca tgaagcctgg ccggatcccc gttactcctt tagtgctatc
     2641 caggcatcga aggggaggga agggggtgct ccccatggcc tgggcgcgaa gttccggggt
     2701 caacgcccgg tcgaaattgg tctggccgag cagggatttt tcgtagctct ggttgtcgat
     2761 ctggtgaata agaacatttt ttgaccagcc gaacttgcgg gtcatgcgca gatagaattc
     2821 ccgctccagc gggtccttgc agcgctccag gatggccagg ttgtggctcc agccgatttc
     2881 tcgccccagt ggtgcgagtt tttcgagtct ggagtatgcc tcggatgacg atatggtgag
     2941 cccaggcaat ttctccaacc agcggttgga gtcttatggc cgctgtattg gggcgcatcc
     3001 taagagtttg cttcaggggc gccgggagcc gtacccccct atgtcatgag cctgatctgc
     3061 cagtccttcg agcggcagat ggacaatggg gaagaggagt gaggccgcct ccccttcaag
     3121 gacagaattt ttgtctgatt agctttgccg cccttcggag tcggcggcat acccgggagc
     3181 tcgtccgcgc gaaagatgcc atgcgccacc gtcgacagac gccgaaagcg acaggtcggt
     3241 tgacagatac cggtcagcgc ggggcgccac cgtgattttg gccaagactt ctttaggacg
     3301 cgccccagcg cggggtgaac cgtcgccggg gcccgtccta agagtttgcc tcagggccgc
     3361 cgggagccgt actccagcca ggtaaggacg acctttttcc gcaggggttc cacatcgtcg
     3421 ctttggcggg ccagggcttg gcggtaggcc tccagggcct ggcggaacgc cagacgcagg
     3481 gcgcggagct cgagggtctc tccgggggga gcctccgccc ccgcctgggc ccggatgacg
     3541 aagagcgttc ccagcacccg gtcgccctgg aggagggtca cggtgcgggt gtcatcctcg
     3601 gagcccggtt ccaggcgcag ggtgagcccc cccagggagc cctcggcgcg ctccggcccc
     3661 tcgtcccgca gcagaaagac ctgc
//
LOCUS       DAOOER010000118         3673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_23655_length_3673_cov_4.812604, whole genome shotgun sequence.
ACCESSION   DAOOER010000118 DAOOER010000000
VERSION     DAOOER010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3673
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23655_length_3673_cov_4.812604"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            315..833
                     /locus_tag="PL162_09415"
     CDS             315..833
                     /locus_tag="PL162_09415"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HQF92275.1"
                     /translation="MGAEMEGHPSPEVWLRGKKQRVRVIPGGFVPAFRAVRREEGGLV
                     VAVSRRCPLSPEEALQEWLQLQAREDLMARVALRGEEMGRHPQRVSVRGQKTRWGCCT
                     RRGTVTLNWRLVMAPPEALDYVVVHELAHLTEPHHRPPFWRRVEAFCPEWRRWRRWLR
                     DFGGQLIVPPLS"
     gene            920..2866
                     /locus_tag="PL162_09420"
     CDS             920..2866
                     /locus_tag="PL162_09420"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HQF92276.1"
                     /translation="MDLRQWDFRGCVLEELPDAAVAALEEAARRGRQRAVTMTAVADS
                     GHPAGSLSSMEMYLLTYGTARLTPENAPSSDRDHVVVSHGHTSPGAYAALAAWGFVDG
                     LDAEAHFRDCGSPFSGHVERGLPGVDWSSGNLGQGLAAGVGYALAARARGNGAHTFVL
                     MGDGGQTKGQSAEARRIAHKEGLGNLTALVDYNGIQISGATGDVMPADLEALWKADGW
                     RVLFCDGHDFRDLYRALREAWHDDKPTVVFCRTVMGKGVSFMEGKADYHGKAPSGELY
                     AQAMRDLGGDPDRLAAAKKRRGAELLPQRCRVRPCLPEMDLGEPKTYDPATKTDCRSA
                     FGEALADVGRRNYGVPGRTPLLVFDCDLAESVKTGAFAKACPDWFIQAGIQEHAVATA
                     AGAASVGGVGALWADFGVFGLSEVYNQQRLNDINETNLKIVLTHVGLDVGEDGMTHQC
                     IDYVGLLRNTFGWKLVVPADPNQTDRALRYALGSWGNLALAMGRSKLPVITREDGTPF
                     FAGDYRFVYGAADVVREGGDVTIVCLGAMLWRGLEARELLAARGISARVVSVSCPLAL
                     GDGDLREMASPGALVTYEDHHVGTGLGAVVTSRMARLGLSCQVATLGVHQYGCSAAAE
                     KVYATMGLTSEDVVETVERLLGRR"
     gene            2866..>3673
                     /locus_tag="PL162_09425"
     CDS             2866..>3673
                     /locus_tag="PL162_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HQF92277.1"
                     /translation="MLLPGDLALLWSPERGDRYLVKVQPGASQGTQLGAVPHALLLER
                     SFGDVVHTHLGHPLMVLRPSLAEFCLRIKRRTQVIYPKEAGYLLMYLHIAPGATVVEC
                     GSGSGGFTTVLAHFVGPQGRVVSYDRRPEFQDIARNNVDRWGVGLQVTFKTKDIEEGF
                     DETEVSAVFLDVPTPWAYLEQAKGALRPGGRLGILVPTFNQIQQSIEALRAGSWVDIE
                     ALEIFLRHLKTNPARLRPEDSMVGHTGYLIFASSVSPEAAQALPWEDRPGE"
BASE COUNT          569 a         1077 c         1388 g          639 t
ORIGIN      
        1 atggccatcc tcaaggccgg agtggcggag tatccggcgg cgaaaaagca ggcggtttct
       61 tcggggagct gctgctgctg cggcggcaaa aactaaatct tccggggcgg cggggacggc
      121 gtaaaaaggc ctagaggatg ccccgggggg cgtagcgctg gggggaaggg gcttgggccc
      181 tttccccctt ttgttctgtg gcgccggggg aggtgggctc ccgaagggga gggtaataag
      241 gccttgagcc ccgcgccgga gtgtgttacc ttgcgaagga cggaacccga cggaagaggg
      301 gcggagcaga aagggtgggg gcggaaatgg agggccatcc atcgccagag gtctggttgc
      361 gggggaaaaa acagcgggtg agggtgatcc ccgggggctt cgtccccgcc tttcgcgccg
      421 tgcgccgcga ggaggggggg ctggtggtgg cggtgtcccg gcgatgtccc ctgtctccgg
      481 aggaggccct gcaggagtgg ctccagctcc aggcccgaga ggacctgatg gcccgggtgg
      541 ccctgcgggg ggaagagatg gggcgccatc cccaaagggt ctccgtgcgg gggcaaaaga
      601 cccggtgggg atgttgtacc cggcggggga cggtgacgct caactggcgt ctggtgatgg
      661 cccctcccga ggccctcgac tacgtggtgg tgcacgagct ggcccacctg acggagcccc
      721 accatcgtcc gcccttctgg cgccgggtgg aggccttttg ccccgagtgg cggcggtggc
      781 ggcggtggct ccgggacttc ggggggcagc tgatagtgcc gcccctttct tgaaagatcc
      841 ccttcaatcc cgaggggcta aagagctatc ggcgtccggg ggggatctcc cggtcgcctt
      901 ttgagaggag agacccggca tggatctgag gcagtgggat tttcgtggat gcgttctgga
      961 ggagctcccc gacgccgccg tggcggcgct ggaggaggcg gcccggcggg ggcggcagcg
     1021 ggcggtgacc atgacggcgg tggcggacag cgggcacccc gcaggatctc tttccagcat
     1081 ggagatgtac cttctgacct atggcacggc ccgcctgacg ccagaaaacg ccccctcgtc
     1141 ggaccgggat cacgtggtgg tgagccatgg ccatacatcc cccggggcct atgcggcgct
     1201 ggcggcctgg ggatttgtgg acggcctgga tgccgaggcc catttccggg actgcggtag
     1261 ccccttttcg gggcatgtgg agcggggcct tcccggggtg gattggagca gcgggaacct
     1321 ggggcagggg ctggcggcgg gggtgggata tgccctggcg gcccgggccc gggggaacgg
     1381 ggcccacacc ttcgtgctca tgggggatgg ggggcagacc aaggggcaat ccgccgaggc
     1441 ccggcgcatc gcccacaagg aggggctggg gaacctcacg gcgttggtgg attataacgg
     1501 catccaaatt tccggcgcca ccggcgacgt catgcccgcc gatctggaag ccctttggaa
     1561 ggccgatggc tggcgggtcc tcttctgcga cggccacgac tttcgagatc tctaccgggc
     1621 cctgcgggag gcctggcatg acgataagcc cacggtggtc ttctgtcgca cggtgatggg
     1681 caagggcgtg agcttcatgg agggcaaggc ggattaccac ggcaaggccc cttcggggga
     1741 gctctatgcc caggccatga gagatctggg gggagatccg gaccgccttg cggcggcgaa
     1801 gaaacggcga ggggcggaac tgttgcccca gaggtgccgt gtgcggccct gcctgccgga
     1861 gatggatctg ggggagccca agacctatga tccggcgacg aagacggact gccggtcggc
     1921 ctttggcgag gccctggccg acgtggggcg ccgaaattac ggtgtgccgg ggcggacgcc
     1981 ccttctagtc ttcgactgcg acctggcgga gtccgtgaag accggggcct ttgccaaggc
     2041 atgccccgac tggttcatcc aggcggggat ccaggagcac gccgtggcca cggcggcggg
     2101 ggccgccagc gtggggggcg tgggggccct gtgggcggat ttcggagtct tcggtctgtc
     2161 ggaggtctac aaccagcagc gcctgaacga catcaacgag acaaatctca agatcgttct
     2221 cacccacgtg gggcttgacg tgggagaaga tggcatgact caccagtgca tcgactacgt
     2281 ggggcttttg cgcaacacct tcgggtggaa gctggtggtg cccgccgatc caaaccagac
     2341 ggatcgggcg ctgcgctacg ccctggggag ctggggaaat ttggccctgg ccatggggcg
     2401 gagcaagctt ccggtgatca cccgggaaga tggcacgccc tttttcgccg gggactaccg
     2461 gttcgtctac ggcgccgccg acgtggtccg agaggggggg gacgtgacca tcgtctgctt
     2521 aggggccatg ctctggcggg gcttggaggc ccgggagctc ctggcggccc ggggaatcag
     2581 cgcccgggtg gtctccgtgt cctgccccct ggcgctgggg gacggagatc tgcgggagat
     2641 ggcctccccc ggcgccctgg tgacctacga ggaccatcac gtggggacgg gcctaggggc
     2701 ggtggtgacg tcccggatgg cccgtttggg cctctcttgc caggtggcca ccctaggggt
     2761 ccaccagtac ggctgttctg cggcggcgga gaaggtctac gccaccatgg gattaacctc
     2821 cgaagatgtg gtggaaacgg tggaacgtct cctggggcgc cgatagtgct tcttcccggc
     2881 gacctggcgc ttctctggtc tcccgagcgg ggggaccgct atctggtgaa ggtccagccc
     2941 ggcgcttccc aggggaccca actgggggcg gtgccccacg cccttttgct ggagcgctcc
     3001 tttggcgatg tggtgcacac ccatctgggg catcctctca tggtgctccg tccctcgctg
     3061 gcggagttct gtcttcgcat caaacgtcgt acccaggtga tctatcccaa ggaggcggga
     3121 tatctcctga tgtatctgca catcgccccc ggagccacgg tggtggagtg cggtagcggc
     3181 tccgggggat ttaccaccgt gctggcccac ttcgtggggc cccaggggcg ggtggtctcc
     3241 tacgaccggc gtccggagtt ccaggatatc gcccggaaca acgtggatcg ttggggcgtg
     3301 gggctccagg tgacttttaa gacgaaagac atcgaagagg gcttcgacga gacggaggtt
     3361 tcagcggtct ttttggacgt gcccaccccc tgggcctatc tggagcaggc caaaggggcc
     3421 ctgcgtcccg ggggtcgtct gggcattctg gtgcccacgt tcaatcaaat tcagcagagt
     3481 atcgaggccc ttcgggcggg gtcctgggtg gacatcgaag cgttggagat ctttttgcgc
     3541 cacctgaaga ccaaccccgc ccggctgcgc cccgaagaca gcatggtggg gcacacgggg
     3601 tacctgatct ttgcctcttc cgtgtccccc gaggcggccc aggccttacc ctgggaggac
     3661 cgtcccgggg agg
//
LOCUS       DAOOER010000119         3660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_23782_length_3660_cov_7.002774, whole genome shotgun sequence.
ACCESSION   DAOOER010000119 DAOOER010000000
VERSION     DAOOER010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23782_length_3660_cov_7.002774"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="PL162_09430"
     CDS             <1..1155
                     /locus_tag="PL162_09430"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HQF92278.1"
                     /translation="FPTRVGRENVLYCHVTLVPYIAVAGEMKTKPTQHSVNELRRIGI
                     QPDIIVCRSQYALEESIKDKIALFCSVAKEAVVEEVDARSIYQVPLSLYEGGVDTLVL
                     RHLGLPSDQSPRLDDWRDYMGRLESSPTVEIAMVGKYMSHKDAYLSVVEALTHAGNHN
                     GVKIQLRPIEAEDVEERGSAVLAGVSGILVPGGFGSRGIEGKIAAASFARREKIPYFG
                     LCLGMHVAVVEFARNVCGIAAAHSSEIDPGTPNPVIHLMEEQRRVSDLGGTMRLGGYA
                     CELLEGSIARQAYGEPQIRERHRHRYEFNNDYRERFEKAGLRPSGFCVGRDLVEIVEL
                     EDHPWFVGVQFHPEFTSRPVRPQPLFRDFVRAAVERSMRDRGPDDKESDE"
     gene            1252..2280
                     /locus_tag="PL162_09435"
     CDS             1252..2280
                     /locus_tag="PL162_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92279.1"
                     /translation="MRKVLLLGLALLGLAAGILSSREAWAEGSTVQILARVESQLYGS
                     PQAGGIVDRLARTEKDLFGRELPGNITDRQQALSTFLEQGSEGQPSLLFKLGVGEWAV
                     GQITYPDDSVSDRIGRLERAIEGKEQEEGALAMRLERVLEMLLGEPVAWESVTVPAGT
                     LIRVELAETLSPKTAKAGDAVKLRLNRDLAVENRLVGARGCLVWGTIESVAPPRSFGR
                     PSEVKIRFDHLFPLGPERVAVNIGENAKRAAQADKAVLAATGASFLGVILLGPLGLAG
                     GLFVRGDEKPLPAGTPFYLEIAQDSSTQSYLVPAGLSGLLTPQILSGDLVEPTPVPRP
                     TRPSENNK"
     assembly_gap    2452..2551
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2644..>3660
                     /locus_tag="PL162_09440"
     CDS             2644..>3660
                     /locus_tag="PL162_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92280.1"
                     /translation="MGRPGWVWVLLGWWLLGVGTGVRGEEFPAGSSDLWGVSSAWGNP
                     APYPGPSGDGETPSREEVRRRVKEILERHTVFHWGEDCLVWMVHYPQELVDPWVALES
                     EKRGLSPEDRAQYRASFVRDLRLGEMEPVLFTVYVFGDSPVSFHPFKERVLLVEASGD
                     VPPLSVESKLDGTLTGIVQGLVFFPKQVGGNFTVKVRPGDAPEFLSFAFKSAPEEPRQ
                     AASPDAPVQVVVAQVPPLPSPVPTPVPPEKPSRRALSPIPIKPTPTVEPPPDFLVLPP
                     VATLGPSPAPAVEVDGPLSPEGVFPSPLSEDPRSSSPGGKEGVGAKESPSPEKERLVE
                     NFLKL"
BASE COUNT          645 a         1027 c         1217 g          671 t
ORIGIN      
        1 tttcccaccc gggtggggag ggagaacgtc ctatactgcc acgtgaccct cgtgccctac
       61 atcgccgtgg cgggagagat gaagaccaag cccacccagc acagcgtgaa cgaacttcgg
      121 agaataggca tccagccgga catcatcgtc tgccgatccc agtacgcctt ggaagaaagc
      181 atcaaagaca agatcgccct cttttgcagc gtggccaagg aagccgtggt ggaggaagtg
      241 gacgcccggt ccatatacca ggtccccctg agcctctatg aagggggggt ggataccctg
      301 gtgctacgcc acctaggcct cccctccgac caatctcccc ggttggacga ctggcgggac
      361 tacatggggc gcttggaatc ttcccccacc gtggagatcg ccatggtggg aaagtacatg
      421 agccataaag acgcctacct cagcgtggtg gaagctctga cccacgcggg gaaccataac
      481 ggagtgaaga ttcagctgcg ccccatcgag gccgaagatg tggaagagag gggatctgcg
      541 gtgctggcgg gggtttcggg catcttggtc cccggaggct tcggctcccg aggcatcgag
      601 gggaaaattg ctgcggcgtc cttcgcccgg agggagaaga tcccctactt tggcctctgt
      661 cttggcatgc acgtggcggt ggtggaattt gcccggaatg tctgtggcat tgcggcggcc
      721 cacagctccg agatagatcc cggcacgccc aatccggtga tccatctcat ggaggaacag
      781 cgccgagtgt ctgacctcgg cggcaccatg cgcctggggg ggtacgcctg tgagctcctg
      841 gaggggagca tcgcccggca ggcctacgga gaaccccaga ttcgggaacg gcatcggcac
      901 cgctacgagt tcaacaacga ctaccgggaa cgcttcgaga aggcggggct ccggccatcg
      961 gggttttgcg tcgggcgaga cctggtggaa atcgtggagt tggaagatca cccctggttt
     1021 gtgggggttc aatttcatcc agaattcaca tctcgtcccg tgcgtcccca acccctgttc
     1081 cgggacttcg tccgggcggc ggtggagcga tccatgcgag atcggggccc tgacgacaaa
     1141 gaaagcgacg agtaaggggt agcgaagggt tgacgcgggc ctccggagct tgcctaaagg
     1201 accagggggg cggcggcctt cccaaacttg atatttggag gcgatgagaa agtgagaaaa
     1261 gtccttctct tagggttggc cctcctgggg ctggcggcgg gaatcctctc ttcccgagag
     1321 gcctgggccg aggggagcac ggtgcaaatt ctggcgaggg tggaatctca gctctatggt
     1381 agcccccagg cggggggtat cgtggatcgt ctggcccgga cggaaaaaga ccttttcggg
     1441 cgggagcttc cgggaaacat caccgatcgc cagcaggccc tttccacttt cctagagcag
     1501 gggagcgagg ggcagccctc cttgctcttc aagctgggag tgggggaatg ggccgtgggc
     1561 cagatcactt atcccgacga ctccgtgtcc gaccgcatcg gccgcctgga aagggccatc
     1621 gagggcaagg aacaggaaga aggagccctg gccatgcgcc tggagcgagt gttggagatg
     1681 cttttaggag agcccgtggc gtgggaatcg gtgacggtgc cggcgggcac gctgatcagg
     1741 gtggagctgg cggagaccct ttccccgaag acggctaaag cgggagatgc ggtgaaactc
     1801 cggctgaatc gggacctggc ggtggaaaat aggctggtgg gggcccgggg atgtctggtg
     1861 tggggaacca tcgagagcgt ggcgcccccc cggagcttcg gccgcccatc ggaggtcaag
     1921 atccgtttcg accacctctt ccccctgggg cccgagcgcg tggccgtgaa tataggcgaa
     1981 aatgctaaaa gggccgccca ggcggataag gccgtcttgg cggccacggg ggcgagtttt
     2041 ttgggagtca tcctcctggg acctctgggg ctggcggggg ggctctttgt ccgaggcgac
     2101 gagaaacccc ttcccgcagg gacgcccttt tacctggaga tcgcccagga ttcctcaacc
     2161 cagagctatc tggtcccggc gggactttcg gggcttttga ccccccagat cttatccggg
     2221 gacctggtcg aacccactcc cgtccctagg ccgactagac ccagcgaaaa caacaagtaa
     2281 gacgacgaaa gaggtggttt ttttatgaaa ctatggacca aaaagttccg tcagacatcg
     2341 tcttcggagg gcggcctttg ccgacgatct cccttttact ctgccctgtc aaaggtgtgg
     2401 gggggagtgc tcctggggat ggtcctgatg ggaccggttc ccgccctggc cnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncgctttaag cgggttcaag gagtgggggt
     2581 ttcggccatc atcgacgtac gtctgtaggg gcgcctgggc ggcgcgatag gagcgcggag
     2641 gatatgggac gcccggggtg ggtatgggtc ctcttggggt ggtggctcct gggagttggg
     2701 acgggagtcc ggggggagga gtttcctgcg ggaagctccg atctttgggg cgtgtcctcc
     2761 gcgtggggta accccgcccc ctatcctggt ccctccggcg acggagagac cccttcgagg
     2821 gaagaggtcc ggcgtcgggt aaaagagatc ctcgagcgcc acacggtctt ccactggggg
     2881 gaggactgcc tcgtctggat ggtgcactac cctcaggagc tggtggatcc ctgggtggcc
     2941 ctggagtcgg aaaaacgagg cctttccccg gaagaccggg cccagtaccg ggcgtctttt
     3001 gttcgagatc tgcgtctggg ggagatggag cccgttcttt ttaccgttta cgtcttcggg
     3061 gactcccccg tatcgtttca ccccttcaag gagcgagtgc tactggtgga agccagcggg
     3121 gacgtgcctc ccctttccgt ggagagcaag ctggatggga cgttgacggg gatcgttcag
     3181 gggctagtat tctttcccaa acaggtcggg ggcaacttca ccgtgaaggt gcgcccgggg
     3241 gatgccccgg agttcttgtc cttcgccttc aaaagcgccc cggaggagcc gagacaagcg
     3301 gcgtctcccg acgcgccggt gcaggtggtg gtggcccaag tgcccccctt gccgtcgccg
     3361 gtacccaccc ccgtgccgcc ggaaaagccc tctcgaaggg ccctttctcc gattccgatc
     3421 aagcccacgc ccacggtgga gcctccgccg gattttctgg tgttgccccc cgtggccacc
     3481 ctggggcctt cgcctgcccc ggcggtagag gtcgatggtc ccctctcccc ggaaggggtc
     3541 tttccatcgc ccctttccga ggacccaagg tcctcttccc ccggagggaa agaaggcgtg
     3601 ggggcgaagg agagcccttc gccggagaag gagcggttgg tggagaattt tctcaagctt
//
LOCUS       DAOOER010000120         3609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_24230_length_3609_cov_10.186269, whole genome shotgun
            sequence.
ACCESSION   DAOOER010000120 DAOOER010000000
VERSION     DAOOER010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24230_length_3609_cov_10.186269"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PL162_09445"
     CDS             <1..214
                     /locus_tag="PL162_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92281.1"
                     /translation="RAEGRAEGIELGKAEGVEQARRENALKMLQDGVSVVNVAKYSGL
                     PEEEVRRLAEETYGGPHCQDKKTADL"
     gene            1149..1367
                     /locus_tag="PL162_09450"
     CDS             1149..1367
                     /locus_tag="PL162_09450"
                     /inference="COORDINATES: protein motif:HMM:NF019487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HQF92282.1"
                     /translation="MASFTSAGSRMTLRVQVGVKANGSPDLRSMTLRGVDAAASADAV
                     HATALALGALLDPPVTEVRKTDDDLVAA"
     gene            1412..1618
                     /locus_tag="PL162_09455"
     CDS             1412..1618
                     /locus_tag="PL162_09455"
                     /inference="COORDINATES: protein motif:HMM:NF022597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HQF92283.1"
                     /translation="MAKTLVLTFLCADGKEWKLNLARPKEGLTKAQCDAAMNAVITQD
                     IFLKAPVSAVEAAYLDRTVTAVTA"
     gene            1748..1897
                     /locus_tag="PL162_09460"
     CDS             1748..1897
                     /locus_tag="PL162_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HQF92284.1"
                     /translation="MEELLRSALQNGFSIAVAAFLLVRMESRLEALTLAINDLRSVLR
                     PGGNS"
     gene            1894..2433
                     /locus_tag="PL162_09465"
     CDS             1894..2433
                     /locus_tag="PL162_09465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQF92285.1"
                     /translation="MTAGPREIPEGEEILRRFEPLVRATARRYAGRGADFDDLVQEGF
                     LALWLALPRCRVPEALPLFLKQSVDRAVRHGAARLRRPDYWVQEEEEEGDEGREFPSD
                     RTREELEAWMVLDALEEEDRVLVTILAAGGTQAEAGRALGLSRATVGFRLGRIRRRWG
                     ALFGDSGLRGEEKGWGHVA"
     gene            2423..2980
                     /locus_tag="PL162_09470"
     CDS             2423..2980
                     /locus_tag="PL162_09470"
                     /inference="COORDINATES: protein motif:HMM:NF017638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 108 family protein"
                     /protein_id="HQF92286.1"
                     /translation="MLPERLANHILRVEGGLADLPADRGGRTYRGITVGTLAEARRDD
                     PSLPGDPADLSEDQVREIYFRRYYLPARCGDLPPPADLLLFDAVVHHGLSGGVRLFQR
                     GINRLMGLHTLAADGVFGPRTLAAARGLVRGELTAPGDRALGVAVLLERAFFFHDFSS
                     RIPAQRVFLPGWLRRLREVAAVAGV"
BASE COUNT          636 a          977 c         1196 g          800 t
ORIGIN      
        1 aagggccgag ggaagggccg agggaattga gttaggcaag gccgagggtg tcgagcaagc
       61 ccgccgcgag aacgctctga agatgttgca ggacggagtg agcgtcgtca acgtcgccaa
      121 gtactccggc ctgcccgaag aggaggtccg acggcttgcg gaggagacgt atggtggccc
      181 ccattgtcaa gacaaaaaaa ccgccgactt atagctactg tacgattctg tgtcttcgat
      241 gaaacggtgc tagatatctc caaggcgctg cggcgcacaa catttccctt aacgcaagaa
      301 gtaaacttct gccggagttt tgtttcctaa cgcctgatag ggtggcagtc attttacgag
      361 atccaaaaaa cggataatct gtgaaaagtg cgttcatgcg gtggcgtaag cgaaatcctt
      421 ccgctgatgg cgccacgggg cgataataca acccgcttcg attcagggct aaaagcctgg
      481 cttgcggcac cgaaggtaag ggtgactttt cgtcccagtc aaggcactct gcgcactaga
      541 ggccttggga gctgtgcgac cacatttttt ttccaaccag tcaagctgca tgggaagccg
      601 cccgacctgg gaggagagtt ctcttatgag ggcatctctg tcatcccgct ccctcttgcg
      661 ggggtcttca aagatcctgg gaagcccttc gagtcattcc ctgcgcactt gggcaggagc
      721 gtgtcatgta cctcatagag ggctgccagt tcacttctag tcctacgatc cgaaaaaatt
      781 aattgatttt tgcgatcctc tgctgacgaa aacttcattc ttaagtaatt ctattactcg
      841 ctttccccgg agctaagggt ctcggtctct ttttctcccg cttcctctgc cgagggatca
      901 ctcttgccaa tcctttctta ccccttgcac agcccccttt cgtcccctca ggcctggctt
      961 acctcggggc cccatccgga gctcctcttt cccctgtgag gcttgctttt ccctttgcgg
     1021 tcgccgataa taagctaaat gcgaaatacg ccttggcatt ttcgccctac ccgcccctct
     1081 attacagaca gaaccacccg gaaggggggc agcccccctt accccttatt ctcgaggagg
     1141 cggatagcat ggcaagtttt acttcggcgg ggagtcggat gaccctgcgg gtgcaggtgg
     1201 gcgtcaaggc caatgggtcg cccgatctgc ggagcatgac cctgagaggt gtcgacgccg
     1261 ccgcttcggc ggatgcggtg cacgccacgg cgctggccct gggggcgctt ttggatcctc
     1321 cggtgacgga ggtgcgtaag accgacgatg atctggtggc ggcctagggc cgtagtcgtc
     1381 gaccatctga ccttgaatag gaggagttct catggctaaa acgctggtgt tgacttttct
     1441 ctgcgccgat ggcaaggagt ggaagctgaa tctcgcccgc cccaaggagg ggctcaccaa
     1501 ggcacagtgt gacgccgcca tgaatgccgt catcacccag gatatcttcc tgaaggcccc
     1561 cgtttcggcg gtggaggccg cgtacctgga tcgtacggtg acggcggtga cggcctagtc
     1621 cctttccccc ggtttatcta ctcttcgtgg gggcaggcct cggggcctgc cccttcttcc
     1681 cgaggggccg gggggcttta gggtttttgt gtttttttag gcctttgtcg aaaggggtgg
     1741 cggtatcatg gaagagttgc tcaggagcgc ccttcagaat ggtttttcca tcgccgtggc
     1801 ggcttttctc ctggtgcgga tggaaagccg gttggaggcc cttaccctgg ctatcaacga
     1861 tcttcggagt gtccttcgcc cgggagggaa ttcatgacgg cggggccccg ggagattccc
     1921 gagggggagg agattttgcg gcgtttcgag ccgttggtgc gggccacggc ccggcgctac
     1981 gcggggcggg gggcggattt tgacgatctg gtgcaggagg gttttttggc cctgtggttg
     2041 gcccttcccc gatgccgggt tcccgaggcc ctgccgcttt ttctgaaaca gagcgtggac
     2101 cgggccgtgc gtcacggggc ggcccggctg cgccgtcccg attattgggt gcaggaggag
     2161 gaggaggaag gggacgaggg gagggaattc ccttcggatc ggacccggga ggagctggag
     2221 gcttggatgg tgttggacgc cctggaagaa gaggaccggg tcctggtgac gatcctggcg
     2281 gcggggggca cccaggcgga ggcgggaaga gctttgggac tttcccgggc gacggtgggc
     2341 tttcggctgg ggcgaattcg gcggcgctgg ggggcgcttt ttggcgattc cggtcttcgg
     2401 ggggaagaaa aggggtgggg tcatgttgcc tgagaggttg gcgaatcata tcttgagggt
     2461 ggagggagga ttggccgatc ttcccgccga tcgagggggg cggacctacc ggggaatcac
     2521 ggtggggacg ctggccgagg cccggcggga tgacccttct ttgcccggcg atccggcgga
     2581 tctttcggag gatcaggtgc gggagatcta ctttcgtcgg tactatcttc cggcccggtg
     2641 cggcgatcta cctcctccgg cggatctgtt gctctttgac gcggtggttc accacggtct
     2701 ttccggggga gtgcggctct ttcagagggg gatcaatcgt ctgatggggc tccacacgct
     2761 ggcggcggac ggggtctttg ggcctcgcac gctggcggcg gcgagggggt tggtgagggg
     2821 agagctcacc gccccggggg atcgggccct gggggtggcg gtgctgctgg agcgggcctt
     2881 tttcttccac gatttttctt cgcgcatccc cgcccagcgg gtctttctac cgggatggct
     2941 ccggcggctt cgggaggtcg cggcggtcgc cggggtctga gcctctgatc cggcgcgaag
     3001 gggggcgtct cgacccacgc tccccccttc gccctgcgag ccgagatctc tcggtaagga
     3061 tcgcatcgcc gagcctatgc cggtgacgcg gaggggaatt cgcaggggcg aaaaacctag
     3121 aagatgatgc ccaacgcaaa aggcttgaag agagccggct ggaagatgcg cactcccggc
     3181 tcgcacgccc ggaggttttc ccggctggag ggaaattcgt aggggcgaaa aacctttcgc
     3241 ccctacttta ttcctgcgtg aaaatcccgg gcatttcctc tatgtcagca tggatgggcg
     3301 cggacgctgc acggacaaca ttttcacgga acgactttgg cgtagctaca aggatgagga
     3361 ggtgtccctg cacgaggacg agtcaccccg ggaatgtcgg caaagggccg ccacatggtt
     3421 tgatttttat aatcacgctc gtccgcatca ggcgttagga aacaaaactc cggcagaagt
     3481 ttacttcttt tgttaaggga aatattgtgc accgcagcgc cttggagatg tctggcgccg
     3541 tttcatcgaa gatgcagaat cgtacagtag ctataagtcg gcggtttttt tgtcttgaca
     3601 atgggggcc
//
LOCUS       DAOOER010000121         3589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_24416_length_3589_cov_6.506791, whole genome shotgun sequence.
ACCESSION   DAOOER010000121 DAOOER010000000
VERSION     DAOOER010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3589
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24416_length_3589_cov_6.506791"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1369)
                     /locus_tag="PL162_09475"
     CDS             complement(<1..1369)
                     /locus_tag="PL162_09475"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HQF92287.1"
                     /translation="MTPSSRSAAPLFLGLDLSTQSLTALALDEGGEVACHRSINFDQG
                     FPSYGTQGGVILDPADPTRIHGDPLLWVEALEEMMRGLGREGLLSRVRAVAVSAQQHG
                     TVYLKPSFAETLLSLAGKGPLKEQMAPTLARRTAPVWMDSSTTKECGEISEALGGDAA
                     VAALTGSRATERFAAPQIRRFFLTHPREYGETGHIALVSSFITSLLAGRIAPVDGGDG
                     FGTNLADISRGEWSPAALDATAPGLLKRLPPLVPRDAVVGPVAPFWTERFGFYPEALV
                     VTGSGDNPCSLVGLGLLENEHPGGISLGTSDTFFAYRRKIPSGERTEGHLFGTADGGF
                     MALLCFKNGSLTRQHVRDTFGLTWEDFSDLLLATPPGNDGKGMIPWRWPEITPRVLRP
                     GVRYFGGLAPDDRAGCVRGVAEAQACALRLHAQGMGPLPESLLVTAGGSENAGLLQIL
                     ADVFGV"
     gene            complement(1366..2532)
                     /gene="gguB"
                     /locus_tag="PL162_09480"
     CDS             complement(1366..2532)
                     /gene="gguB"
                     /locus_tag="PL162_09480"
                     /inference="COORDINATES: protein motif:HMM:NF040906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HQF92288.1"
                     /translation="MQKAKELFLTNIRQYAMMVALVFIVLLFQYLTDGVLLRPLNLTN
                     IIQQNSYILVLAIGMLLCILTGNIDLSVGSVAAFVGAVGATMMAVWKWPVIPSIFLCL
                     LLGILIGCWHGFWISYFRIPAFIVTLAGMLCFRGLTLVMLKGQTIAPMPELFRQLSSD
                     FIPDFLGAGGLNITALALVGAVLLAFAFFDLRDRTHKLAYGFDVLPLPLWMLKIILLG
                     AVVMAFAHQLALHKGIPMVLLLLGFLVLVYSFITQKTVFGRHVYAFGGNEKAAKLSGI
                     DTDRVFFLVYANMGLLAAVAGLVFAGRLNAASPKAGMNFELDAIAACFIGGASASGGI
                     GTVAGAIIGGLIMGVLNNGMSIIGVSIDWQQVIKGLVLLGAVAFDVYTKNKAAK"
     gene            complement(2532..>3589)
                     /locus_tag="PL162_09485"
     CDS             complement(2532..>3589)
                     /locus_tag="PL162_09485"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HQF92289.1"
                     /translation="LLILDEPTAALNDEDSENLLQLLLELKGQGITSIIISHKLNEVE
                     RIADRITVIRDGAVIETLDNGERNVKEDRIIKGMVGREITDRFPPRPPAERAEVALEV
                     EDWVVYDPLFLDRQKIRGVSFSVHKGEVVGIVGLMGAGRTELAMSLFGQSYGHRISGR
                     MRLNGREVTLPSPKEAIRHGLAYLTEDRKSAGLILMDDVRHNISLASLGKLSNRQGVI
                     DDNKEITVAEDLRHKLRIKTPTIFQKIGHLSGGNQQKAILARWIYADPDVLILDEPTR
                     GIDVGAKYEIYGIINELVALGKAVILISSEMPEVLGMSDRIYVMNEGRFVGELSREEA
                     SQESIMRCIMQSNREVA"
BASE COUNT          670 a         1151 c         1162 g          606 t
ORIGIN      
        1 gcaccccgaa gacatcggcc agaatttgaa gaagaccggc gttttccgac ccccccgcag
       61 tgaccagaag actttccggg aggggaccca tgccctgggc atggagccgc agggcgcagg
      121 cctgggcctc cgccacgccc cgaacgcatc ccgccctgtc atccggcgcc agccctccga
      181 aatagcgcac ccccggtcga agcacccggg gggtgatttc cggccaccgc caggggatca
      241 tccctttgcc gtcgtttccc ggcggcgtgg caaggagcag atcggaaaaa tcttcccagg
      301 tcaagccgaa ggtatcccgc acgtgctgtc gggtaagact tccgtttttg aagcagagca
      361 acgccatgaa ccccccgtcg gcggtgccga agaggtgtcc ctcggtgcgc tccccggatg
      421 gaatcttccg gcggtaggca aaaaacgtgt cgctggtgcc caggctgatg ccgccggggt
      481 gctcgttttc caggagcccc agccccacca ggctgcacgg attgtccccc gaccccgtga
      541 ccacgagggc ttcggggtaa aatccgaagc gctccgtcca gaagggggcc acggggccca
      601 ccaccgcatc ccggggtacg agggggggca aacgcttcag gagccccgga gcggtggcgt
      661 cgagggccgc cgggctccac tccccccggg agatgtccgc cagattggtg ccgaagccgt
      721 cccctccgtc caccggggcg atgcgccccg ccagcagcga cgtgatgaaa gaactcacca
      781 gggcgatgtg ccccgtttcc ccgtattccc gggggtgggt cagaaaaaaa cgccgaatct
      841 ggggagcggc gaagcgctcc gtggcccggg atcccgtgag ggccgccacg gcggcgtccc
      901 cccccagggc ctcggagatc tccccgcatt ccttcgtcgt gctggaatcc atccacaccg
      961 gcgcggtgcg ccgggcgagg gtcggagcca tctgctcctt gagcggcccc ttcccggcga
     1021 gggaaaggag cgtttcggcg aaagagggtt tcaggtacac cgtgccatgc tgctgggcgg
     1081 agaccgccac ggcccgcacc cgggagagaa gcccttcccg accgaggccc cgcatcattt
     1141 cctccagggc ttccacccaa agaagggggt cgccgtggat ccgggtgggg tccgccgggt
     1201 ccaaaatcac gcccccttgg gtgccgtagg aggggaaccc ctggtcgaag ttgatcgacc
     1261 ggtgacaggc cacctcaccg ccctcgtcca gggccagggc ggtgaggctc tgggtgctca
     1321 ggtcaagtcc cagaaagagg ggggcggcgg aacgcgagga gggcgtcatt tggcggcctt
     1381 gttcttggta tagacgtcaa aggccacggc ccccaggagc accagcccct tgatcacctg
     1441 ctgccagtcg atggagaccc cgatgatgga catgccgttg ttgagcaccc ccatgatgag
     1501 gccgccaatg atggcccccg ccaccgtgcc gatgcccccc gaggccgaag cccccccgat
     1561 gaagcacgcg gcgatggcgt ccagctcgaa gttcatcccc gccttgggag aggcggcgtt
     1621 gaggcgcccg gcgaaaacca gccccgccac cgccgccagg agtcccatgt tggcgtagac
     1681 caggaagaac acccggtcgg tgtcgatgcc ggagagcttg gcggcctttt cgttgccgcc
     1741 gaaggcgtag acgtggcgcc cgaagacggt cttctgggtg ataaacgaat agaccagcac
     1801 cagaaagccc agcagcagga gcaccatggg aattcccttg tgcagggcga gctggtgggc
     1861 gaaggccatg accacggcgc ctaagagaat gattttcagc atccacaggg gcagggggag
     1921 cacgtcgaag ccgtaggcga gcttgtgggt gcggtcccgg aggtcaaaga aggcaaaggc
     1981 caaaagcacc gcccccacca aagccagggc ggtgatgtta agtcctccgg cgccgaggaa
     2041 atcgggaata aagtccgagc tcaactgccg gaagagctcc ggcatggggg cgatggtctg
     2101 cccttttagc atcaccaggg tgagcccccg aaagcagagc atccccgcca gggtgacgat
     2161 aaaggctgga atgcggaagt aagaaatcca gaagccgtgc caacagccga tgaggatccc
     2221 cagaaggagg cataagaaga tcgagggaat cacgggccac ttccacaccg ccatcatggt
     2281 ggcccccacg gcgcccacga aggccgccac cgagcccacc gagagatcga tgttccccgt
     2341 gaggatgcac agcaacatgc cgatggcgag aacgagaatg tagctgttct gctggatgat
     2401 gttcgtcagg ttcagggggc gcaggagcac cccgtcggtg agatattgaa acaacaggac
     2461 gatgaagacc aaggccacca tcatggcgta ttggcggatg ttggtcagga agagttcttt
     2521 cgccttttgc atcacgccac ctcccggttg ctttgcataa tgcaccgcat gatgctctcc
     2581 tgggaggcct cttcccggga aagctccccc acgaaacgcc cttcgttcat gacgtagatc
     2641 cggtcgctca tgcccagcac ctcgggcatc tccgaggaga tgagaatcac cgcttttccc
     2701 agggccacca gctcgttgat gatgccgtag atctcgtact tggcccccac gtcgatgccc
     2761 cgggtgggct cgtccaggat cagcacgtcg gggtccgcgt agatccatcg ggccaggatg
     2821 gccttctgct gatttccccc cgacaggtgg ccgatcttct ggaagatggt cggcgtcttg
     2881 atgcggagct tatggcgcag atcctccgcc accgtgattt ccttgttgtc gtcgatgacc
     2941 ccctggcggt tgctcagctt tcccaggctg gccagggaaa tgttgtgtcg gacgtcgtcc
     3001 atgaggatga gcccggcgga ctttcggtcc tcggtgaggt acgccaggcc gtgccggatg
     3061 gcctccttgg gcgaggggag ggtcacctcc cggccgttta ggcgcatcct tccggagatg
     3121 cggtggccgt aggactggcc gaagaggctc atggccagct cggtgcgccc cgcccccatg
     3181 aggcccacaa tgcccaccac ctcgcccttg tggaccgaga agctgacccc tcggatcttt
     3241 tgacggtcca gaaagagggg gtcgtagacc acccagtctt ccacttcgag ggccacctcg
     3301 gcgcgttctg ctggggggcg cggagggaag cggtccgtga tctcccgccc caccatgccc
     3361 ttgatgattc ggtcctcctt gacgttgcgt tccccgttgt ccagggtttc gatgacggct
     3421 ccgtcgcgga tcaccgtgat ccggtccgcg atgcgctcca cctcgttgag cttgtgggaa
     3481 atgatgatgg acgtgatgcc ctgccccttg agctccagca ggagctggag cagattttcg
     3541 ctgtcttcgt cgttcagggc ggcggtgggc tcgtcgagga tgagcaggc
//
LOCUS       DAOOER010000122         3513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_25113_length_3513_cov_8.613071, whole genome shotgun sequence.
ACCESSION   DAOOER010000122 DAOOER010000000
VERSION     DAOOER010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25113_length_3513_cov_8.613071"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..1228)
                     /locus_tag="PL162_09490"
     CDS             complement(212..1228)
                     /locus_tag="PL162_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQF92290.1"
                     /translation="MTWKRGLALGTLLVALWGGSFVGAEAGESLKAYTTLEEAYAKEL
                     LDAFEKETGIAVEWVRLASGETVARIEAEQANPQASIWVGGVGTLHIDAKNKGLTAPY
                     TSRVAENIPAKYRDPDRFWIGLYVGPIAFAMNTDRAKELGLTMPKTWADLLKPEFAKK
                     VRMAHPSTSGTAYNVVTTLIRIFGGDEDKAFDFMKKLDGAIDQYTKSGSAPGKNCAIG
                     EIPVAIGYLHDLIKLKVTGAPLEIVVPEDGTGFETASLSLIKGGPDAVNAKKLYDWIL
                     GESAAKIIASWYVIPLSQKAPALDTGFGLEKMNLVDQDDVWDAANKARLLERWQNELG
                     AVAK"
     gene            complement(1393..2364)
                     /locus_tag="PL162_09495"
     CDS             complement(1393..2364)
                     /locus_tag="PL162_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HQF92291.1"
                     /translation="MEKTSLGWTLEERGRRAARVTGVGLVLNAGLTAFKYVAGFWGHS
                     GAMVADATHSLSDLVTDVVVLLGFRVVQKPSDQGHDFGHGKVETLLALICGLFLLGAG
                     LGICGNALWALERVYQGEVLRAPEGVAFVAAVVSVVSKELLYWYTLYWGKRLASPALV
                     AKAWDHRSDAFSSVGTTLGIGGAILLGEEWRVLDPLAALVVSVFVVKVSLPMIRESVN
                     ELLESSLSEEEEGRIEEILRRPAVVRDVHGLRTRKIGFYVALEAHVLMARHLSLVEAH
                     DVATLIEKDLRRELGEHTLASLHVEPEPPEGESHEEGHVEAMVRFLE"
     regulatory      complement(2414..2480)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02683"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="NiCo riboswitches sense nickel or cobalt ions;
                     Derived by automated computational analysis using gene
                     prediction method: cmsearch."
                     /bound_moiety="nickel or cobalt"
                     /db_xref="RFAM:RF02683"
     gene            complement(2707..3252)
                     /gene="yfcE"
                     /locus_tag="PL162_09500"
     CDS             complement(2707..3252)
                     /gene="yfcE"
                     /locus_tag="PL162_09500"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HQF92292.1"
                     /translation="MTTRLGIVSDTHGSLTAWENAQKLWGPVEGVLHAGDVFYARADR
                     PLPPGFAPDELAAALNACPVPLLLARGNCDSPADEGKLRWPLGAVVSAWWRGRLILVE
                     HGEDFSAFRERALGCGAALAISGHTHVASLVREGGTIFLNPGSASLPLGRDPASVALV
                     DEEELTIVTLSGVVLHREPWG"
     gene            complement(3259..>3513)
                     /locus_tag="PL162_09505"
     CDS             complement(3259..>3513)
                     /locus_tag="PL162_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HQF92293.1"
                     /translation="LGKGVCGTAAAERRTVLVPDVEAFPGHIACDGASASEIVVPLLT
                     EGILRGVLDLDSPEKGRFDEEDARGLEDFAARLVRMVRWS"
BASE COUNT          657 a         1275 c          975 g          606 t
ORIGIN      
        1 caccccgtaa accccgaggg cgaaaaggac caacgccacc gctgtgctcc tcatcttccg
       61 ccccggccca tcggagcccc ggggcccctc gttcctgcgc aaggagcctt ccccctctct
      121 cttccccctc tttcgccggg atcgcccgga agaccttcaa tcggcggggc cgatcgggcc
      181 agccccgccg acgcttcgat ctgtcctacc gctacttcgc cacggctccc aactcgttct
      241 gccaccgctc caggagacgg gccttgttcg ccgcatccca gacatcgtcc tgatccacca
      301 ggttcatctt ctccaaccca aaccccgtat ccagggcggg ggccttctgg gacaggggaa
      361 tgacgtacca actggcaatg atcttcgccg ccgattcccc caggatccag tcgtagagtt
      421 tcttcgcgtt caccgcgtcg gggccgcctt tgatgagcga gagggaagcc gtctcaaaac
      481 ccgtgccatc ctcgggaacc acgatctcca ggggagcccc cgtcaccttg agcttgatga
      541 gatcgtgcaa gtatccgatg gccacaggga tctccccgat ggcacagttt ttccccggag
      601 ccgacccgga cttggtgtac tgatcgatgg ctccgtcaag ttttttcatg aaatcaaagg
      661 ccttgtcttc gtccccaccg aagatccgga tcagcgtggt aaccacgtta taggccgttc
      721 ccgaggtgct gggatgggcc atgcgcactt tctttgcaaa ctccggcttc aacaaatcgg
      781 cccaggtctt gggcatggtg agccccagct ccttcgcccg gtccgtgttc atggcaaagg
      841 cgatgggtcc cacgtagagg ccaatccaga agcggtcggg atcccggtac ttcgcgggga
      901 tgttttccgc cacccgagaa gtgtaggggg ccgtgagtcc cttgtttttg gcgtcaatgt
      961 gcagcgtgcc cacgccgcca acccaaatgg aagcctgggg attggcctgc tccgcctcaa
     1021 tgcgagccac ggtctctccc gaagccagac ggacccactc caccgcgatg cccgtctcct
     1081 tttcgaaggc atccagcaat tccttggcgt aggcttcctc cagggtggtg taagctttta
     1141 ggctctctcc ggcctccgcc cctacgaaac ttccgcccca caaggccacc agcaacgtcc
     1201 ccagcgccaa acccctcttc cacgtcatga cacagccccc ttctcgatgc ggatcggggg
     1261 gatatccccc cgggccaatg tatatcaaat tcccggaagg ggcaaggttc catcgccccg
     1321 ggggaccttc aaaaaacggg aaaggagagc gccagaaggg ggatcgtcgt ccctttcgtg
     1381 gttccccttc gcctattcca ggaatcgcac catggcctcc acgtgacctt cttcgtggct
     1441 ttccccctcg gggggttcgg gctccacgtg gaggctggcc agcgtgtgct ctcccaactc
     1501 tcgccgcagg tctttttcaa tgagagtggc cacgtcgtgg gcctccacca aagacaggtg
     1561 gcgggccatg agcacgtggg cctccagggc cacgtaaaac ccgatctttc tcgtccgaag
     1621 gccgtggacg tcgcggacga cggcggggcg gcgcaagatc tcctcgatac gcccctcttc
     1681 ttcttcgctc agagagctct ccaacagctc gttcacgctc tcccggatca tgggaaggga
     1741 gaccttaact acgaaaacgc tcaccaccag tgccgccagg ggatccagca cgcgccactc
     1801 ctctcccagc aaaatggccc ccccgatccc cagcgtggtg ccaacggagg aaaaggcgtc
     1861 ggaacggtgg tcccacgcct tggccaccag ggcggggctc gcgagccgtt ttccccagta
     1921 gagggtgtac cagtacagca gttccttgga gaccaccgag accaccgccg ccacaaaggc
     1981 cacaccttcc ggcgcccgaa ggacctctcc ctgatatact ctttccaggg cccaaagggc
     2041 gttcccgcag atgcccagcc ccgcccctag aaggaagagg ccgcagatta gcgccagcag
     2101 agtctccacc ttgccgtggc caaagtcgtg tccttggtcc gagggtttct ggacgacccg
     2161 aaaccccaga agaaccacca cgtcggtgac cagatccgag agggaatggg tggcatccgc
     2221 caccatggcg ccgctatgcc cccaaaaacc cgccacgtac ttgaaggcgg tgagccccgc
     2281 gttcagcacc aatccgaccc ccgtcacccg ggcggcccga cgtccccgtt cttccagcgt
     2341 ccaacccagg gatgtcttct ccatgataat cctcctttcc ccggagagcg caaaagcccc
     2401 ccaggacaca acggtcccgc gggggctttc ccgctgccca aagggcccgg ccgcatgcct
     2461 ctcaaaggca ccgcctgctc tcctcaagaa caacgtggtt caaagcacca ttctatcggg
     2521 aaaaggactc ccttcgcaag ccctttaccc catcctcgac ctccgctccg gggaaaattc
     2581 taaaaaaatc ttttatgcac ctcggcgtag gcattgcctc ccgggcaagg gccccatcaa
     2641 ccccacgccc tcgtcggtgc cgtgaagcct cctcgtcacc cccggccaaa aaacggatgg
     2701 gcgatcttag ccccagggct cccggtgcaa caccaccccc gagagggtga cgatggtcag
     2761 ttcctcctcg tccaccagcg ccacgctggc ggggtcccgc cccagcggga ggctcgcgga
     2821 accggggttg aggaagatgg tgcctccctc ccgcaccagg gacgccacgt gggtatgccc
     2881 ggaaatggcc agggcggcgc cacaccccag agcccgctcc cgaaaggcgg aaaaatcctc
     2941 gccatgttcc acgaggatga ggcgcccccg ccaccacgcc gacaccacgg cccccagagg
     3001 ccagcgcagc ttgccctcgt ccgccgggct gtcgcagttt ccccgggcca gaagcagagg
     3061 cacggggcag gcgttcaggg cggcggccaa ttcgtcgggg gcaaatcccg gaggaagggg
     3121 gcggtcagcc cgggcataaa acacgtcccc cgcgtggagc accccctcca ccgggcccca
     3181 gagcttttgg gcgttctccc aggccgtcag gctcccgtgg gtgtctgaaa caattcccag
     3241 acgggtcgtc accgtcgttc agctccaccg caccatgcgg accaaccggg cggcgaagtc
     3301 ctccagcccc cgggcatcct cttcgtcgaa acgccccttt tcggggctgt ccagatcgag
     3361 caccccccgg aggatcccct ccgtcagcag gggcaccacg atctccgacg ccgaggcgcc
     3421 atcgcaggcg atgtgccccg gaaaggcctc cacatcgggc acgaggacgg tgcgcctctc
     3481 cgcagcggcg gtgccgcaga cccccttgcc cag
//
LOCUS       DAOOER010000123         3513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_25114_length_3513_cov_7.486119, whole genome shotgun sequence.
ACCESSION   DAOOER010000123 DAOOER010000000
VERSION     DAOOER010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25114_length_3513_cov_7.486119"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2397
                     /locus_tag="PL162_09510"
     CDS             <1..2397
                     /locus_tag="PL162_09510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQF92294.1"
                     /translation="AVSWGPETWKKSENRLRILSRAIEESPVGVLIADPHGTIEYANP
                     RFLEMTGFPLQELVGQTPRVLKSGRQDEAFYRDLWDTILDGREWRGELCNKRKDGSFY
                     WARASIYPVRCAKGNVTHFVAIQVDVTKALEAEAELARARDAADAANRAKSAFLARMS
                     HEIRTPMNGILGMAEILQGTELSSRQHRYLDIIQTSAESLLDIINDILDLSKIEAGAM
                     TLIPAPFDLLKTAEDVMLLMAARARSKGLELVLRYDPETPRRVVGDGGRLRQVMVNLV
                     GNAVKFTDEGHVVLGITSSPLGGGRMTLYLRVADTGVGIAPEDQSRIFEPFSQADNTS
                     SRRYQGTGLGLSISRQLVDLMGGSMAVQSRLGEGTTFSVAIPAGMAPEAEETEERSVT
                     PVIPGKRVLVVDDVAPNRLLLEEVLKTRGFDVISVDSAPRARHALWAAEREGRPFSLL
                     LTDRHMPGEDGLALVRSLPGILQVVPPCVLLSSLGDEEEEEEKEPLLAEVMTKPLRQT
                     QIDAMLGRIFLGGERGGEKTLPEGGERLSLGLRVLLAEDHPVNQEVGRALLARAGCTA
                     DVAENGKEAVRRYQESPGRYDVILMDCQMPEMDGYEATRRIRALEEGGRHLPILALTA
                     SATEDERRRCLDAGMDDVLAKPISLSALTEALGRYAKEGEEGSEGGVLPPEAPKAAPP
                     SPVDRSVLADEVGDDPELVGELYRHFLATHREDMALMEKTLAEGDREALRRAAHRIKG
                     AAASVGARPLAKASEDLERHGLEGREEEILALAERVRQSSQEVQRFLELSGETPGDGA
                     "
     gene            2486..>3513
                     /locus_tag="PL162_09515"
     CDS             2486..>3513
                     /locus_tag="PL162_09515"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing response regulator"
                     /protein_id="HQF92295.1"
                     /translation="MADTTEDPMTSLRCLVVDDHPFQRKVVRTMLLRLGINQVTEASD
                     GKEALEQVGAETPDVVLCDLQMPSMDGVELLRHLGQGGYEGGVILMSGVDPRVLATAE
                     MVARSYRLTLLGALPKPVQSGPLRELLSRARRQGVPSRETAPPATPPLSPEEVRRALD
                     RGEIAPFFQPMVQISSGEIVSAEALARWRREGVEVASPGAFIPVAEEHGLMPLLTEKM
                     LEESLKHLSTWKSRGIDLSVSVNFSAVDLRDLSLPDRTEAAAAAWGVSPESVTVELTE
                     SRVIEDLAQALDVLVRFRMKGFELAMDDFGTGSATLEQLRRIPFTKIKIDQMFVTGAA
                     SSRDRRIML"
BASE COUNT          640 a         1046 c         1233 g          594 t
ORIGIN      
        1 gcggtctcgt ggggaccgga aacctggaaa aagtcggaaa accgcctgag gatcctgtcc
       61 cgagccatcg aagagagccc cgtgggggtc ctgatcgccg atccccacgg caccatcgaa
      121 tacgcgaatc ctcgcttcct ggagatgacg ggctttcccc ttcaagagct cgtcggccag
      181 acccctcggg tgctcaaatc cggccgccag gacgaggcct tttatcgcga cttgtgggat
      241 accatcctcg atgggcggga gtggcgcgga gagctctgca acaagcgcaa ggatggcagt
      301 ttttactggg cccgggcctc catctacccc gtgaggtgcg ccaagggcaa tgtcacccat
      361 ttcgtggcga tccaggtgga tgtcaccaag gccctcgaag cggaggcgga gctggcccgg
      421 gcccgagacg ccgccgatgc ggccaaccgc gccaagagcg catttctggc ccggatgagc
      481 catgagatcc gcacccccat gaatggcatc ctcggcatgg cggagatcct ccaggggacg
      541 gagctctcct cccggcaaca ccgttacttg gatatcatcc agacctcggc ggaatctctg
      601 ctggacatca tcaacgacat attggacctg tccaagatcg aagcgggggc catgaccctc
      661 attccggcgc cctttgacct cctcaagacg gcggaggacg tgatgctcct catggcggcc
      721 cgggcccggt ccaaggggtt ggagctggtg ttgcgctacg acccggagac cccccggaga
      781 gtggtgggag atggggggcg cctgcggcag gtgatggtca atctggtggg caatgcggtg
      841 aagttcaccg atgagggcca tgtggtgctg ggcatcacct cctctcctct ggggggtggg
      901 cggatgactt tgtaccttcg tgtggcggac acgggggtgg ggattgcccc cgaagatcag
      961 tcccggatct tcgagccctt ctcccaggcg gataacacct cgtcccgccg ataccagggg
     1021 acgggactgg gtctatccat cagccgccag ctggtggatc tcatgggggg gagcatggcg
     1081 gtacaaagtc gtttggggga ggggacgacc ttttccgtgg cgatccccgc agggatggcg
     1141 cccgaagccg aagagaccga agaaagaagt gtcacgccgg tgattcccgg gaaacgggtc
     1201 ctggtggtgg acgacgtggc ccccaatcgg ctgctgcttg aagaagtttt gaagacccga
     1261 ggatttgacg tgatcagcgt ggacagcgcc ccgagggcgc gccacgccct atgggccgcc
     1321 gaaagggaag ggcgcccctt ttccctgctt ctcaccgatc ggcatatgcc cggagaggac
     1381 ggcttggcat tggtgcgatc cctcccgggc atcctccagg tcgtgcctcc ctgcgttctc
     1441 ctctcctccc tgggggacga ggaagaagag gaggagaagg agcccctcct cgcggaggtg
     1501 atgaccaaac ccctgcgcca gacccagatc gatgccatgc tggggcggat ctttctcggg
     1561 ggagaaagag ggggagaaaa aaccctcccg gaggggggag agcgcctctc cttgggtctt
     1621 cgcgtgctcc tggcggagga tcaccccgtg aaccaagagg tgggccgagc tctcctggcc
     1681 cgggcgggct gtacggcgga cgtcgccgaa aacggcaaag aagcggtgcg gcgttaccag
     1741 gaaagtccgg gacgctatga cgtcatcctc atggattgcc agatgcctga aatggacggc
     1801 tacgaggcca cgcgacggat tcgggctctg gaagaggggg ggcgacattt acccattctg
     1861 gccctcaccg ccagcgctac ggaggacgag cggcggcgtt gcctcgatgc ggggatggac
     1921 gacgtgctgg ccaagcccat ttccctgagc gccctgacgg aggccctggg gcgctacgcc
     1981 aaggagggtg aggagggctc cgagggagga gtccttcccc ccgaagcccc gaaggccgcg
     2041 cccccttcgc ccgtagatcg gagcgtgctg gccgacgagg tgggtgatga tcccgaactg
     2101 gtgggggagc tctatcgcca ttttttggcg acgcaccggg aggacatggc gctgatggaa
     2161 aagaccttgg cggaggggga tcgggaggcg ctgcgccggg cggctcaccg gatcaagggg
     2221 gcggcggcct ccgtgggggc tcgccccctg gcgaaggcca gcgaagatct ggaaaggcac
     2281 ggcctggagg ggcgtgaaga agaaatcctt gccctggcag aaagggtgcg ccagagctct
     2341 caggaggtgc agcgcttcct ggaattgtcg ggggagactc cgggggatgg cgcttaaagc
     2401 cccccggagg gactgggtcg gagtcttgtg gtaaataagg gcacccgagg ggcgccgaag
     2461 cgacgataga tgggggagaa gaggcatggc cgatacgacc gaggatccta tgacgtcttt
     2521 gcgatgcttg gtggtggacg accacccctt ccaacggaag gtggtgcgca ccatgctcct
     2581 ccgcctgggg ataaaccaag tgacggaggc aagcgatggg aaagaagccc tggagcaggt
     2641 gggggccgaa acccccgatg tggtgctctg cgacctccag atgccttcca tggacggcgt
     2701 ggagctgttg cgccacctgg ggcagggggg gtatgagggg ggggtgatcc tcatgagcgg
     2761 cgtcgacccc cgggtcctgg ccacggcgga gatggtggcc cggtcctatc gcctcaccct
     2821 tctgggagcc ctgcccaagc ccgtgcagag cggccctctc cgggagcttt tgtcccgggc
     2881 ccggaggcaa ggggtcccat cgagggagac cgcccctccg gcgaccccgc ccctttcccc
     2941 ggaggaggtg cgccgggccc tggatcgggg ggagatcgcc cccttctttc agcccatggt
     3001 ccagatctct tcgggggaga tcgtttcggc ggaggccctg gcccgatggc gtcgggaagg
     3061 cgtcgaggtg gcctccccag gggcctttat ccccgtggcg gaggaacacg gtctcatgcc
     3121 ccttctgacg gaaaaaatgc tggaagaatc cctgaagcac ttaagcacct ggaaatctcg
     3181 gggaatagat ctttccgtgt cggtaaactt ctccgccgtg gatttgcggg atctttccct
     3241 ccccgaccgc accgaggcgg cggcggcggc ctggggggta tcgccggagt ccgtcacggt
     3301 ggagctgacg gaaagccgcg tcatcgaaga cctggcccag gccctggacg tactggtgcg
     3361 atttcgcatg aagggtttcg agctcgccat ggacgacttc ggcacgggga gcgccacgtt
     3421 ggagcaactg cggcgcatcc cctttaccaa gatcaagatc gaccagatgt tcgtcacggg
     3481 ggccgcctcg tcccgagacc ggaggatcat gct
//
LOCUS       DAOOER010000124         3507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_25183_length_3507_cov_7.161645, whole genome shotgun sequence.
ACCESSION   DAOOER010000124 DAOOER010000000
VERSION     DAOOER010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3507
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25183_length_3507_cov_7.161645"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="PL162_09520"
     CDS             <1..464
                     /locus_tag="PL162_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQF92296.1"
                     /translation="RLIPWLTVRQNLAFVSPDDPMPLLSRLGLGDHADAFPQELSGGM
                     RQRVNLARALLVNPDLMLLDEAFSSLDVGIKIGILEDLGHLWRRHPFALVVVTHNPRE
                     ALLLADRILVIRGHPAKISEEIVVSLPEDRSYADPAVNALEAEVLKKILQG"
     regulatory      539..685
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            677..1492
                     /locus_tag="PL162_09525"
     CDS             677..1492
                     /locus_tag="PL162_09525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQF92297.1"
                     /translation="MKGHASWMVSSQGSPPSPTEALHAVVAETDPMLRSLYRNVVLEN
                     SHYVTMGSVDTGKALFLLLARAPAHLVLLDVFLPSFGGPQGLRRLRQDFPRTDFIVLS
                     SGDQPDVVREALCIGAFDFLVKPFPFDRLRRALEAYAFFHYGLTKKSGPWCQEELDSL
                     LQYRRGKAEDRRIKEDFPREAFPPKGLQAKLLDAFCAILQSSPTSLSAREAGLMLRVA
                     RSTARRYLEHLVDTGKAGFVYGYREVGRPVKSYHSLVPGKQNRTQQTISVPFS"
     gene            1595..>3507
                     /locus_tag="PL162_09530"
     CDS             1595..>3507
                     /locus_tag="PL162_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQF92298.1"
                     /translation="MEQYLRKRIVVNGVPRDVVAKGEASLAHIIRKQLGLTGTKVGCE
                     CGQCGACNVILDGKVTRSCIVKWESIRPEASVLTIEGIGAPGRLHALQWAFVKTGAIQ
                     CGFCTPGFIVSAKGLLDENPSPTREEVRDWFQKGRTACRCTGYRQIVDGVMLAARVLR
                     GEESMTDFSEMRGRDGAVWGTAYPRPSAVHKATGLTSYGDDLALQLPEETLHAVPVCP
                     ETHHARVKQIDFSEAEKMPGVYKIVTAKDLLANKGTNRIRGQVGSKTASTDGWERRIL
                     VEEGDKIRQWGEPVALVCADTEDHARAAAAKVRVELEELRPLLSVREAMAPGAPKVYD
                     EVPDIDGIPNCFNKRPIIKGEDPAPALDGAPFVVEQEFYSSRQPHLTIETDCGFGYYD
                     EEGRLTLQTKSICVYRHQLMIARGIGLAPSKIRVISNPMGASFGYKVAPTNEPLLAAC
                     VLATGKPVYMRLNMKEHITRTPKRSPFLMRVRVGADDHGKIVGLEHHWYVDHGPYSES
                     SQDLTNKGGQFMFAPYRLDHIRGVGYTFFTNHRWCGAFRAYGAPQTYLAGEIAVDMLA
                     EKVGLDPLDFREKNLLQPGDTLPSGQRAEVYPLQAMVTRLRPLYDEAKRRAREDSTET
                     VRRGVGVAIGIYNA"
BASE COUNT          675 a         1099 c         1053 g          680 t
ORIGIN      
        1 cccgcctaat cccttggctc accgtccgac agaacctcgc ctttgtctcc cccgacgacc
       61 ccatgccctt gctctcccgc ttgggcctgg gagatcacgc tgacgccttc ccccaggagc
      121 tcagcggagg catgcgtcag cgggttaacc tcgcccgggc cctgttggta aaccccgacc
      181 tcatgctcct ggacgaggcc ttctcctccc tggacgtggg cataaaaatc ggcatcctcg
      241 aggatctggg ccatctgtgg cgacgccacc ccttcgccct ggtcgtggtt acccataacc
      301 cccgggaagc cctcctcctc gcggatcgta tcttagtgat ccgagggcac cccgcgaaga
      361 tatcggaaga gatcgtcgta tcccttcccg aggaccggtc ctacgcagat cccgcagtga
      421 acgctctgga ggcggaggtt ttaaaaaaaa tcctccaggg atgaccttct cgcccggtat
      481 cgatgaaagg ccttttcttg cccctccctt tcgggtatga tggcgcaata tagtatgtat
      541 atcgttccac ctccgagccc gaccctccta aaggtcatgc ctcaatccag acccaaggat
      601 aagggccgtt agggtgtttc tggaaacggg aatgccttcc gcatgtgcat ccgcatgttt
      661 gaaaaggagg atttttatga aaggccacgc gtcatggatg gtgtcgtccc aagggagtcc
      721 tccatcgccg acggaggctc ttcacgccgt cgtcgccgag accgatccca tgctccggtc
      781 gctgtaccgg aacgtggtgt tggagaactc tcattatgtg accatgggga gcgtcgacac
      841 aggaaaggcc ctcttcttgc tcctggcccg agctccggcc cacctcgtac ttcttgacgt
      901 cttcctcccg agcttcgggg gacctcaggg gctccgtcgc ctgcgccaag acttccctcg
      961 gacggatttc atcgtgcttt cttcgggaga ccaacccgat gtggtgcgag aggcgctctg
     1021 tatcggagcc tttgactttc tggtcaagcc ctttcccttc gatcgcctgc gacgagctct
     1081 ggaggcctac gcgtttttcc attatgggct aacaaaaaag tccggtccct ggtgccaaga
     1141 agaactggat tccctcttgc aataccggcg aggcaaggca gaagatcgcc ggatcaagga
     1201 agatttcccc cgagaggcct ttccccccaa gggtcttcag gcgaagctcc tggatgcctt
     1261 ttgtgccatc ctccagtcct ccccgacgtc cctttcggcc cgggaggcgg gcctcatgct
     1321 ccgcgtggcc cgatccacgg cccgccgcta cctggagcac ctcgtcgaca cgggcaaggc
     1381 gggctttgtt tatgggtacc gggaggtggg gcgccccgtc aagagctatc actccttggt
     1441 gcccggtaaa caaaatagga cacaacagac catatccgtg cccttttctt gagtctttac
     1501 gcggggctcc ctatgcttac gagggcgccg gggtctttgg cgctccccgg ctcggcgtcg
     1561 ggggagaatt cgggcagatg ggggcggaat ggtcatggag cagtatctcc gtaagcggat
     1621 tgtggtaaat ggagttcccc gggatgtggt ggccaaaggg gaggcttccc tggcccacat
     1681 tatccggaaa cagctcggcc tcaccggtac caaggtgggg tgcgaatgcg gccagtgcgg
     1741 ggcctgtaac gtcatcctcg acggcaaggt cactcgttcc tgcattgtca aatgggaatc
     1801 gatacgcccc gaagcttcgg tgctcaccat cgaagggatc ggggccccgg gacggctcca
     1861 cgcgctccag tgggcctttg tgaaaaccgg cgccattcag tgtggctttt gtaccccggg
     1921 attcatcgtc agcgccaagg gattgctaga tgaaaaccct tccccgaccc gggaagaggt
     1981 ccgggattgg ttccagaaag ggcgcaccgc atgccgttgc acgggatatc gccagatcgt
     2041 ggacggagtc atgctcgccg ccagggtcct gcggggcgaa gaaagcatga cggatttttc
     2101 cgagatgcgg gggcgcgacg gcgccgtgtg gggtacggcc tatcctcgcc ccagcgcggt
     2161 ccataaggcc acggggctta cctcctacgg cgatgacctg gcccttcagc tccccgaaga
     2221 aaccctccac gcggttcccg tgtgcccgga aacgcaccat gcccgggtga agcagatcga
     2281 tttctccgag gccgaaaaga tgcccggggt gtacaagatc gtcacggcca aggacctcct
     2341 ggcaaacaag ggaactaacc gcattcgcgg ccaggtgggg tcgaagaccg cgtccaccga
     2401 tggatgggag cggcgcatcc tggtggaaga gggagataag attcgccagt ggggggaacc
     2461 ggtggccttg gtatgcgccg acacggagga ccacgctcgg gccgccgccg ccaaggtacg
     2521 agtggagcta gaagagcttc ggcccctgct ttccgtccga gaggccatgg cccccggggc
     2581 tcccaaggta tacgacgagg tgcccgatat cgacggtatc cccaactgct tcaacaagcg
     2641 ccccatcatc aagggagagg atcccgctcc ggcgttggac ggagccccct tcgtggtgga
     2701 gcaggagttt tacagctctc ggcagcccca cctcaccatc gaaacggact gcggcttcgg
     2761 gtactatgac gaagaagggc ggctcacgct tcagaccaag agcatttgcg tctaccgcca
     2821 tcagctcatg atcgcccggg gcattggctt ggctcccagc aagattcgag tcatctccaa
     2881 ccccatgggg gcgtcctttg gctacaaggt ggctcccacc aatgagccgc tcctggcggc
     2941 ctgcgtcctg gccacgggaa aaccggtgta catgcgcctc aacatgaaag agcacatcac
     3001 ccggaccccc aaacgctccc ccttcctgat gagggtccgc gtgggggccg acgatcatgg
     3061 caaaatcgtg gggctggaac accactggta cgtggatcac ggcccctact ccgagtccag
     3121 ccaggatctc accaacaagg gaggacagtt catgttcgct ccctaccgtc tcgaccacat
     3181 ccggggggta ggttacacat ttttcaccaa ccatcggtgg tgtggcgcct tccgggccta
     3241 tggggctccc cagacctacc tggcgggaga gatcgccgtg gacatgctgg cggaaaaagt
     3301 aggcctggat cccctggatt tccgggagaa gaacttgctc caaccgggag acacccttcc
     3361 gagcgggcaa agggccgaag tctaccccct gcaggccatg gtaactcggt tgcggcctct
     3421 gtacgacgag gcgaaaaggc gagcccggga ggactccacc gagacggtac ggcggggtgt
     3481 gggggtggcc atagggatct ataacgc
//
LOCUS       DAOOER010000125         3502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_25226_length_3502_cov_6.241659, whole genome shotgun sequence.
ACCESSION   DAOOER010000125 DAOOER010000000
VERSION     DAOOER010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25226_length_3502_cov_6.241659"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1836)
                     /locus_tag="PL162_09535"
     CDS             complement(<1..1836)
                     /locus_tag="PL162_09535"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQF92299.1"
                     /translation="MTPQGRSSEEAFLEELRRDFSLEAEEHLQTIVSGLLELEKDPAH
                     PVVEKVYRAAHSLKGAAHAVQMPLVATLCQAMEGVLSALKKGLLFLRAEDFDVLQRSV
                     DAVGRMVAEGEGQGLDGSLVRELEMLTEARRGPGKETGEEGRRKAPLPPSPGGEGLFK
                     AGDPGVIHPPAFPEAPMPPPLVAGVPEVEVVSAVRGGASQTIRISAARLDALLLQAEE
                     LISVKLALHQRAEDLEGVIASLSAWKKDLEKADQEGRREEGAPGPSEEARREYRRRLR
                     EVEDALGHLRKSLVMDASEAGTLIRHLQEGTREALLRPFSSMLQSFPKMVRDIARDLD
                     KAVDLEIHGDDIEVDKRILEGLKDPLIHLVRNAMDHGMERPGERRAQGKPEKGTLSLL
                     VSRESGSRVELMVKDDGRGIDPDKVREGAVKAGIISLHEAQALDRGSALRLIFRSGFS
                     TSPIITSLSGRGLGMAIVQEGVERLGGRLTLDSTPGQGTTFCISLPLTLATFRGVLVR
                     EWGRIFVVPTAQVGRVTRVGEESFRRVENREVVVIQGEPMSCFRLGLVLGLPEQSDDK
                     VAGILPRVMGASRRPGRTLVILSTGETPLALEVDGVENEQEILLKP"
     gene            complement(1912..>3502)
                     /locus_tag="PL162_09540"
     CDS             complement(1912..>3502)
                     /locus_tag="PL162_09540"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQF92300.1"
                     /translation="LEFIGMARIPDLKNMGALNYLCAIARSQAQAGAQALDLEESGRR
                     DEALKELQKDREATFQKMDAAWEGIKAIPRQSEKGHLIMAALTAQYARWKEQHKALDQ
                     LLDEASRALPAHRRQELRRQYHVSLDALLPFSETMGKTVRDLVDNNAKNTDRFIREDL
                     AAAEWIKSASAIVTGLGLAMTILLGWAITRGVSAPIGVGVSLLARMGRGDLTQDVPPE
                     FISRGDEIGDLARAVQELSEDLRLQVTALKDAASVLGTAASQIAASAAQVTAGAEETA
                     VAVMETTTSVEEIRATAEATTRKTREVAEHTQQGLHLVQGGRKATEDLTEGIQRISTQ
                     MAFIAETIVKLSEQSQEIGEITGTVEELAEQSNLLAVNAAVEAARAGDQGRGFSVVAQ
                     EIKSLAEQSKEAAKRVQRILKDIQKATAAAVMATEQGSKAVDQARASAPSRESLQGMS
                     RSFAESAQSAAQIAAANAELLSGVDQAAQAMASIREAGHQNVEGMREMENAAQSLKDM
                     GQRIADLVGRYALKGDEERDG"
BASE COUNT          543 a         1244 c         1024 g          691 t
ORIGIN      
        1 gggtttgaga agtatttctt gctcgttttc caccccatcc acctcaaggg ccaggggcgt
       61 ctcccccgta ctcaaaatca ccaacgtcct cccgggacgg cgggacgccc ccatcaccct
      121 cggcaggatc cccgccacct tatcgtcgct ctgttccggt agtcccagca ccaagcccag
      181 gcggaaacag gacatgggct ccccttgaat caccaccacc tcacgattct ccacccgacg
      241 gaagctctct tcgcccaccc gcgtcacccg ccccacctgg gccgtgggta ccacgaagat
      301 ccttccccac tcccgtacca ggacccctcg gaaggtggcc aaagtcaggg gaagggagat
      361 gcaaaaggtc gtcccctggc ccggcgtgga atccagggtg aggcgtcctc cgaggcgttc
      421 caccccctct tggacgatgg ccatgccgag ccctctcccg gaaaggctgg taatgatggg
      481 gctggtggaa aaacccgaac gaaagatcag ccgaagggcg gagccccgat ccagcgcctg
      541 ggcctcgtgg agggagatga tccccgcctt gaccgccccc tcccgcacct tatccgggtc
      601 gatgccccgg ccatcgtctt tcaccatgag ctccacccgg ctcccgctct cccgggaaac
      661 cagaagcgac agggtgcctt tctcgggctt cccttgggcc cggcgctccc cgggcctttc
      721 catgccgtga tccatggcgt tacgcaccag atggatcagg gggtctttca gcccttccag
      781 gatacgctta tctacctcga tgtcgtctcc gtggatctcc aaatccacgg ccttgtccag
      841 atcccgggcg atgtcccgca ccattttggg aaagctctgg agcatcgagg agaaaggacg
      901 gagcagggct tcccgggtgc cctcctggag atgacgaatc agcgtccccg cttcggaggc
      961 gtccatcacc agggatttcc tgagatggcc caaggcatct tccacctccc ggaggcgacg
     1021 gcggtactcc cgccgcgcct cctccgaggg acccggggct ccttcttccc ggcgtccctc
     1081 ttgatcagcc ttttccaagt cctttttcca agccgaaagc gaggcgatga cgccttccag
     1141 atcttctgcc cgttgatgaa gagccaactt tacggagatg agctcctccg cctggaggag
     1201 cagggcgtct aacctcgcgg cgctgatgcg gatggtctgg gacgcccccc ctcgaaccgc
     1261 cgaaaccacc tcgacctccg ggactccggc gacgagggga gggggcatgg gggcttcagg
     1321 gaaagccggt ggatggatca ccccgggatc tcccgccttg aaaaggcctt cgcccccagg
     1381 agaagggggc aaaggggcct tcctgcggcc ctcctctccc gtttccttcc cgggcccccg
     1441 tcgcgcctcc gtcagcattt ccagctctct gacgaggctc ccgtcaagcc cctgcccttc
     1501 tccttccgcc accatgcgcc ccacagcgtc gacgctgcgc tgcagcacgt cgaaatcctc
     1561 ggcccggagg aagaggagac ccttttttag agccgatagg acgccctcca tggcctggca
     1621 cagcgtggcc accaggggca tctgcacggc atgggccgcc cccttgaggc tatgggccgc
     1681 tcggtagact ttttccacca ccggatgagc cgggtctttt tccagttcaa gaaggcccga
     1741 cacgatggtc tggaggtgct cttccgcttc cagggaaaag tcgcggcgga gctcctccag
     1801 gaacgcctcc tccgacgaac gaccctgtgg cgtcacgggc ccaccccctt agtcccgggc
     1861 cccgggcaaa gggccatcgt cttctgtcgg ggatctttcg ccccgtgttg cctatccatc
     1921 cctctcctca tcgcccttca gcgcataacg ccccaccaga tcggcgatcc gctgccccat
     1981 gtcttttaag ctctgggcgg cgttttccat ctccctcatg ccttccacgt tctggtggcc
     2041 cgcctcgcgg atgctcgcca tggcctgggc cgcctggtcc acccccgaca ggagctcggc
     2101 attggccgca gcgatctggg cggcggactg ggcggattcg gcaaagctcc ggctcatgcc
     2161 ctggagggac tctcgagagg gggccgaggc tctcgcctga tccacggcct tggagccctg
     2221 ctccgtggcc atgaccgccg ccgcagtggc tttttgtatg tcttttaata tgcgctgaac
     2281 cctcttggcc gcttccttgg attgctccgc cagactcttt atctcctggg ccaccacgga
     2341 aaatccccgc ccctgatccc cggcccgggc ggcctccacg gcggcgttca ccgccagcag
     2401 gttggattgc tccgccagct cctccaccgt gccagtgatc tctccgatct cctggctctg
     2461 ctcgctgagc ttgacgatgg tttccgcgat gaaagccatc tgggtgctga tgcgctggat
     2521 gccctccgtc aggtcctccg tggccttgcg tcccccttgg accagatgga gcccctgttg
     2581 ggtgtgttcc gccacctccc gggtcttgcg ggtcgtggcc tccgccgtgg cccggatctc
     2641 ttccacgctc gtcgtggtct ccatcaccgc caccgccgtc tcttcggccc cggcggtgac
     2701 ctgggccgcc gaggcggcga tctggctcgc cgccgttccc aggaccgacg ccgcgtcttt
     2761 cagggcggtc acctgtaacc gcaggtcctc ggagagctcc tgaacggccc gggccaggtc
     2821 gccgatctcg tccccccggg aaatgaattc cggggggacg tcctgggtca ggtcgccccg
     2881 ccccatgcgc gccagaaggg agacccccac gccgatgggg gcgctgacgc cccgggtgat
     2941 ggcccacccc aggaggatcg tcatcgccag gccaaggccg gtgacgatcg cgctggcact
     3001 tttgatccac tccgccgccg ccaggtcctc ccggataaat cggtccgtgt tttttgcgtt
     3061 attatcgacc agatccctca cggtcttacc catggtttcg gaaaatggta ggagggcatc
     3121 taaagagaca tggtactgtc ggcggagctc ctggcgccgg tgggccggaa gggcgcggct
     3181 cgcctcgtcg aggagttggt ccagggcctt atgctgttct ttccagcggg cgtattgggc
     3241 cgtcagcgcc gccatgatga ggtgcccctt ctccgactgc cgagggatgg ccttaatacc
     3301 ctcccaggcg gcatccatct tctgaaaagt ggcttcgcga tctttctgga gttcctttag
     3361 ggcttcgtcg cggcgcccgg attcctccag gtccagggct tgagctccgg cctgggcctg
     3421 ggaccgggct atggcacaga gatagttgag cgcccccatg ttttttaggt cggggatacg
     3481 cgccatcccg atgaactcca ag
//
LOCUS       DAOOER010000126         3471 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_25552_length_3471_cov_8.543618, whole genome shotgun sequence.
ACCESSION   DAOOER010000126 DAOOER010000000
VERSION     DAOOER010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25552_length_3471_cov_8.543618"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PL162_09545"
     CDS             <1..298
                     /locus_tag="PL162_09545"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HQF92301.1"
                     /translation="LKKAGVSPEGVRGYDVQCATHLEGAARVASGMADAVLGLRAAAE
                     ALDLGFVPLAEEPFELVIPCRHLSHPGILALFRAMEDISWRRAVEAMGGYRWPS"
     gene            286..1302
                     /gene="moaA"
                     /locus_tag="PL162_09550"
     CDS             286..1302
                     /gene="moaA"
                     /locus_tag="PL162_09550"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HQF92302.1"
                     /translation="MALLTDRCGRTLTYLRVSLTDRCNLRCRYCMPEEGVPEVPHEEI
                     LSFEEVERLCALFSLMGIRKVRFTGGEPLVRRGVLAFLASFRRQLPHLRCALTTNGLL
                     LAPMAPDLLSLGLAGINVSLDTLNHDKYRYITRWGDLNQVFRGIEAVQGHIHIKINTV
                     LIRGFNEGEIESLVHFAKDHGATLRFIEFMPLDGSVWSPDSFLSAEDMRLLLPDPGNW
                     RPEESFPLIFPEGLTPEGEGVTESDAGPAKYYRHVHTGQRVGTIAAVSSHFCRTCNRL
                     RLSATGDLRTCLFQNQSLSLREALRAGDDSRVMEMVREAVRDKPLSWQNLRDSERHMS
                     RIGG"
     gene            1303..>1770
                     /gene="moaC"
                     /locus_tag="PL162_09555"
     CDS             1303..>1770
                     /gene="moaC"
                     /locus_tag="PL162_09555"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HQF92303.1"
                     /translation="MMSFTHMDGEGHPVMVDVGEKLPSAREARAEGWVRLPQGVYDAI
                     ASREVKKGDVLGVAEIAGIQGAKATASLIPLCHPLRLDEVRVRCRLDSCARAVHVESV
                     VRARDVTGVEMEALTAVSLAALTVYDMCKAIDKGMAIDGVRLTYKSGGKSGTYR"
     assembly_gap    1771..1870
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1871..2648
                     /locus_tag="PL162_09560"
     CDS             <1871..2648
                     /locus_tag="PL162_09560"
                     /inference="COORDINATES: protein motif:HMM:NF013183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HQF92304.1"
                     /translation="MELLLGRDQGLVRAKAMVWGDASLELEALLIDDPVPDGGALWVV
                     SDRIFHAPPGSFISFGPSGAVLRRGEGRDLRVWHPGILSFSRPISLWRPLRVGVLTVS
                     DKGSRGERRDTAGPALEAAARAIGGDVTCRQVLPDEEDLLVKTITSWADQDECHIILI
                     TGGTGFSPRDLTPEALEKVATRFVPGLGEAMRQVSSGRSPRAPLSRGGAVLRNQTLIL
                     ALPGSERGAVECFRAVAPLLRHGVEIAQGWEGECGGHGHA"
     gene            2635..>3471
                     /gene="rapZ"
                     /locus_tag="PL162_09565"
     CDS             2635..>3471
                     /gene="rapZ"
                     /locus_tag="PL162_09565"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HQF92305.1"
                     /translation="MDTLNSSVAESRISPREVSRCIIITGLSGGGKSATLNVFEDQGM
                     FAIDNIPPALMPQLLELLRTHPAAVEHGVAAVMDVRGGELLHDLVEVMGHLRRMLPQT
                     QLVFLDAEDGELLKRFETTRRRHPLGNVPILEGVAQERELLAPVREIADVVVDTSGLT
                     LTSFREKLLASVGGFHENPTVVLTSFGFKYGTPKDCDFLFDARFLPNPYYEKNLRHLS
                     GLDQPVREFLDSIIETRVLLGHLYPLLDSIIPLYGNVGKMQMHVAVGCTGGRHRSVAL
                     VEW"
BASE COUNT          630 a          922 c         1107 g          712 t
ORIGIN      
        1 cctgaaaaag gccggagtgt cccccgaggg ggtgcgaggc tacgacgtac agtgcgctac
       61 gcacttggag ggggcggctc gggtagcctc cgggatggcg gacgcggtgt tgggccttag
      121 ggccgctgct gaggcactgg acctgggttt tgtccccctg gcggaagaac cctttgaatt
      181 ggtcatcccc tgtcgccacc tatcacaccc ggggattttg gccttgttcc gcgccatgga
      241 agacatatct tggaggaggg ccgtggaggc catgggaggt taccgatggc cctcctgacg
      301 gaccgttgcg ggcgcacgtt gacctatcta cgggtatcgc tgacggatcg atgcaacctg
      361 cgctgcaggt actgcatgcc cgaagagggg gtgccggagg tgccccacga ggagatcctt
      421 tccttcgaag aggtggaacg cctctgtgcc ctcttttccc tgatgggcat ccgcaaggtg
      481 cgttttactg gaggagagcc cttggtccgc cggggagtgt tggcctttct cgcgtccttt
      541 cgccgccagc taccccacct tcgatgcgcc ttgaccacca atggccttct gctggctccc
      601 atggcgcccg atctcctttc cctgggcctg gcgggcataa acgtgagcct agatacgcta
      661 aatcatgata aatatcgtta cataacccga tggggggact taaatcaagt ttttcggggc
      721 atagaggccg ttcaaggaca cattcacatt aaaataaaca cggttcttat ccgtggtttt
      781 aacgaaggtg agatagaaag tttggtgcac ttcgcgaaag accatggggc cacgttgcgt
      841 tttatcgagt tcatgccctt ggatggctcc gtttggtctc cggactcctt cctttcggcg
      901 gaggacatgc gcctcctcct gcctgacccg ggaaactgga ggcccgaaga gagctttccc
      961 ctcattttcc cggaggggtt gactccggag ggggagggag tgacggagag cgatgcgggg
     1021 cccgccaaat actaccgcca cgtccacacg ggacagcgag tggggaccat tgccgccgta
     1081 agttctcatt tttgccgtac gtgtaaccgg ttgcgcctgt ctgccacagg agatctacgc
     1141 acctgcctgt tccagaatca gagcctgtcc cttcgcgagg cgctacgtgc cggggatgac
     1201 tcccgggtca tggaaatggt gcgcgaggcc gtgagggata aacctctttc ctggcagaac
     1261 ctccgggact ccgagcggca catgtcccgc ataggaggat agatcatgtc tttcacacat
     1321 atggatggag agggccatcc cgtcatggtg gatgtgggcg aaaaactccc ttcggcgagg
     1381 gaagcccggg cggaagggtg ggtgcgcctg ccccagggcg tgtatgacgc cattgcctcc
     1441 cgggaagtga aaaaaggcga cgtcttgggg gtggcggaaa tagcgggaat ccaaggagcc
     1501 aaggccactg cgtcgctaat ccccctgtgt cacccccttc gcctcgacga agtgcgggtg
     1561 cggtgtcgtc tggactcctg tgcccgagca gtccacgtgg agagcgtggt gcgggcccgc
     1621 gacgtgaccg gggtggaaat ggaggccctc acggctgttt ccttggcggc cctgacggtg
     1681 tacgatatgt gcaaggccat agataaaggc atggcgatcg acggcgtgag gctcacgtat
     1741 aaaagcggtg gaaagagcgg gacctaccgt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn aatggaactt ctcctggggc gcgaccaggg cttggttcgc gcaaaggcga
     1921 tggtgtgggg agatgcctct ctggagctgg aagcgcttct tatcgacgat cccgtccccg
     1981 atgggggggc gctctgggtg gtgtctgata ggatttttca cgcccccccg gggagcttta
     2041 tatcctttgg cccctccggg gcggtgttgc gtcgtggaga gggaagagat ctgcgtgtgt
     2101 ggcatccggg tatcctgagt ttttcccgcc ccatctccct gtggcgtcct ttgcgggtcg
     2161 gagtgttgac cgtgagcgac aaggggagcc gaggagaacg gcgggacacg gcgggaccgg
     2221 ccctggaagc ggcggcgagg gccataggag gagacgtgac ctgtcgccag gtgttgcccg
     2281 acgaagaaga tcttttggta aaaaccatca cttcctgggc ggaccaggac gaatgtcata
     2341 ttattctgat cacgggagga acggggtttt ctcctcggga tctaacccct gaggccctag
     2401 aaaaagtggc cacgcgcttc gtgcccggcc tgggcgaggc catgcgccag gtttcgtcgg
     2461 ggcgctctcc ccgggctccc ctgtcccgtg ggggggcggt gctgaggaat cagacgctga
     2521 tcttggccct tccagggagc gagcgagggg cggtggagtg ctttcgggcg gtggcccctc
     2581 tgctacggca cggcgtggaa atagctcagg gttgggaagg ggagtgcgga ggccatggac
     2641 acgcttaact cttccgtcgc tgagtctcgc atcagcccca gagaagtgag ccgctgtatc
     2701 atcatcaccg gcctatccgg aggaggcaaa tccgccactc tcaacgtctt tgaagaccag
     2761 ggaatgtttg ccatagacaa catacctccg gctctgatgc ctcaactctt agaactgctg
     2821 cgcacccatc cggcggcggt ggaacacggc gtggcagcgg tgatggacgt ccgaggaggg
     2881 gagctcctcc acgacctggt ggaggtcatg ggacacctcc ggaggatgct accgcaaacg
     2941 caactggtgt ttctggacgc agaggacggg gagctcttaa agcgttttga gaccacccga
     3001 cgacggcacc ccctgggcaa tgtccccatt ctggaaggag tggcccagga gcgggagctt
     3061 ctcgccccgg tgagggagat cgccgacgtg gtggtggaca cctcagggtt gactttgacg
     3121 tcttttcgag agaagctcct ggccagcgtg ggtggatttc atgaaaatcc tacggtggtt
     3181 cttacgtctt ttggattcaa atacggcacc ccgaaggact gcgacttcct cttcgatgcc
     3241 cgttttcttc ccaatccata ctatgaaaaa aatcttagac atctctcggg attagatcag
     3301 ccggtgcgag agtttttgga tagcattatt gagacccggg tgctgttggg ccatctttac
     3361 cctctgctgg actccatcat tcccctgtat ggcaacgtgg ggaagatgca aatgcacgta
     3421 gcggtggggt gcactggggg gcgtcaccga tcggtggcgt tggtggagtg g
//
LOCUS       DAOOER010000127         3401 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_26274_length_3401_cov_4.839809, whole genome shotgun sequence.
ACCESSION   DAOOER010000127 DAOOER010000000
VERSION     DAOOER010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3401
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26274_length_3401_cov_4.839809"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PL162_09570"
     CDS             complement(<1..190)
                     /locus_tag="PL162_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92306.1"
                     /translation="MGWAGLAWGIPASPLPVEVFQPDGTRMELTNRGDEFCSWVETPE
                     GYTVIQNSGSGFWEYARKK"
     gene            418..996
                     /locus_tag="PL162_09575"
     CDS             418..996
                     /locus_tag="PL162_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HQF92307.1"
                     /translation="MILAASAIAAGVREGAIVIDPFDPRFLGPNSYDLHLGSRIARLA
                     APLVDPLTPTAYEECSFRDTYTAFPGELYLAHTVERAGSAVHVPKIEGKSTLARLGLS
                     IHVTAGYGDVGFVGAWTLEITTLRPFVLHAGMPICQIEFSTLLRKEGEEDRLYAGAYS
                     GLEGIGLPRPRRDWVFLTPSRSGNTQDGGTLP"
     gene            complement(993..1226)
                     /locus_tag="PL162_09580"
     CDS             complement(993..1226)
                     /locus_tag="PL162_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92308.1"
                     /translation="MMDMGQEPIFSEFSPVLAPGGGAGGRRRPIPGLRCADGEEERRA
                     ERHWREVGRGGGENPEDPPAPEDRERWRERRLS"
     gene            complement(1223..2311)
                     /locus_tag="PL162_09585"
     CDS             complement(1223..2311)
                     /locus_tag="PL162_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HQF92309.1"
                     /translation="MEHPAPGALPAGGSPPGIMTDRAPGFAPPAVVPTITDILRARIF
                     LAPRVRRTPLEPSPVLSELVGVPVFLKCENLQRCGSFKIRGSLYRMEHLSPEERRRGV
                     VTASSGNHGQGVALAAATLGVAATVVVPQSCPETKRAAILHRGKAWVELRVTEGLYDD
                     AEREAHRLAEQEGRCYISSFEDRFVAAGAGTVGWEILEEEPALGALVSPLGGGGLLSG
                     IAVASRALGPGMALWGVTARANPCWARAWDRGRMEPVEDEKESLAEGLAGTGSPRLYP
                     FLREHLAGVRSVTEDEIARAMALLHRAHHLVVEGAGAVGVAALLTGVIPLSGPVAVVL
                     TGGNVDEEPFLEALKRGTKRQRAEGGLA"
     gene            complement(2254..>3401)
                     /gene="argH"
                     /locus_tag="PL162_09590"
     CDS             complement(2254..>3401)
                     /gene="argH"
                     /locus_tag="PL162_09590"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008709378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HQF92310.1"
                     /translation="HMGRSRNDQVATALRLYLRGAILGLQDALDDLLSVLLGRAQSHA
                     EVLVPGYTHLQQAQPVALGHYWMAHFWAFLRDDKRFSAAHDAADECPLGSGALAGSTL
                     PLDRTFVARDLGFSRVTDNSMDTVASRDYLLDTLYAAASFGIHASRLAEDLIVYASTE
                     FGWIRLPDAFCTGSSMMPQKKNPDVLELIRGKAGQFVGHLVDLMTTTKGLPLTYNRDL
                     QEDKRGLWGALDTAAAVLDVLPPLLARVEVDEPRAREAFRDGYLLSTDVAEFLVRRGV
                     PFRTAHEKTGRAVRWCLDRQRPLASLSLEEWQALIPEASEELLPLLTPESAVRRRSTL
                     GGTAPESVQRQILHGQEVLAHRRMKRERRWNTLPRELSRRGDRPRES"
BASE COUNT          540 a         1208 c         1112 g          541 t
ORIGIN      
        1 gtttctttcg ggcgtactcc caaaaaccgc ttccgctgtt ctggatcacc gtgtatccct
       61 cgggggtctc cacccaggag caaaactcgt ccccccggtt cgtcaattcc atgcgggtcc
      121 cgtcgggctg aaagacttcc acgggaaggg ggctggcggg aatcccccag gccaggcccg
      181 cccaccccac cagaactcca gggtccccac gaattccttc atcgccccca cctcttttcg
      241 ataacggctg tcctttaaga cgccgaaagg gtcatacacc aaacagcctc gactgtaaag
      301 gcccctcggg agtcgcccga agggttttct ttagattcct tggccaaggc gggggaaagg
      361 ggggggaaaa tcgtgtatgc ttaggggcat ccatcggcgc cttgaggagg aatgatcatg
      421 attcttgccg cttcggccat cgctgcgggg gtccgggagg gggccatcgt catcgatccc
      481 ttcgaccccc gtttcctggg gcccaactcc tacgatctgc acctgggatc ccggatcgcc
      541 cgtctcgccg ctcccctggt ggaccccctg acgcccacgg cgtacgagga gtgctccttc
      601 cgggacacgt acaccgcctt tcccggggaa ctctacctgg cccatacggt ggagcgggcg
      661 gggtccgccg tccacgtccc caagatcgag gggaaatcca ccctggcccg gctggggctt
      721 tccatccacg tcactgcggg gtacggcgac gtggggttcg tgggggcctg gaccctggag
      781 atcaccaccc tgcgcccctt cgtgctccat gcggggatgc ccatctgtca gattgagttt
      841 tccactctgc tccggaaaga gggagaggaa gaccgactct atgcgggcgc gtacagcggc
      901 ctggagggca tcggccttcc ccgcccccgg agggactggg tctttctgac tccctccagg
      961 agcggaaata cccaagacgg tgggaccctt ccctaggaga ggcgccgctc ccgccaccgc
     1021 tcccggtcct ccggcgcggg gggatcttcg ggattttctc ctcccccacg ccccacctcg
     1081 cgccagtggc gctcggcccg gcgctcctcc tcgccgtcgg cgcagcgaag cccggggatg
     1141 gggcgtcggc gtcctccggc tccgcccccc ggggcaagaa cgggggaaaa ctccgaaaag
     1201 atgggctcct ggcccatgtc catcatgcca aacctccctc ggccctttgg cgctttgttc
     1261 cccgtttcag ggcctccaga aagggctcct cgtccacgtt gcccccggtg agtaccaccg
     1321 ccacggggcc cgagaggggg atgacccccg tcaagagagc ggccaccccc acggcccccg
     1381 ccccttccac caccaggtgg tgagcccgat gaagcagggc catggcccgg gcgatctcgt
     1441 cttccgtcac gcttcggacc cccgcaaggt gctcccgaag gaaggggtag agccgggggg
     1501 accccgtgcc cgccaaccct tcggcgaggc tttctttttc gtcttccacc ggctccatgc
     1561 gcccccgatc ccaggcccgg gcccagcagg gattggcccg ggcggtgacc ccccagaggg
     1621 ccatgccggg gcccagggcc cgggaggcca cggcgatccc cgagaggagc cccccgcccc
     1681 ccagggggct caccaaagcc cccagggcgg gctcttcttc caggatctcc catcccacgg
     1741 tgcccgcccc tgccgccacg aagcggtcct cgaaggagga gatgtagcac cgcccttctt
     1801 gctccgccag ccggtgggcc tcgcgctccg cgtcgtcgta gagcccctcc gtcacccgga
     1861 gctccaccca ggccttgccc cgatggagga tggcggcgcg cttggtctcg ggacagctct
     1921 ggggcaccac caccgtggcg gccactccta gggtggcggc ggccagggcc actccctggc
     1981 cgtgattccc cgaggaggcg gtgaccaccc ctctccggcg ctcctccggc gacaggtgtt
     2041 ccatgcgata aagggaaccc cggatcttga aggatccgca gcgctggagg ttttcacact
     2101 tcaggaacac cggcaccccc acgagctccg agagcaccgg ggagggttcc aagggggtcc
     2161 ggcgcacccg gggggccaaa aagatccggg cccgcaggat gtcggtgatg gtggggacca
     2221 cggcgggagg ggcgaacccc ggcgctcggt cggtcatgat tcccgggggc gatccccccg
     2281 ccgggagagc tcccggggca gggtgttcca ccgccgctcc cttttcatcc gacggtgcgc
     2341 cagcacctct tgcccatgaa ggatctggcg ttgcacggat tccggcgcag tcccccctag
     2401 ggtggagcgg cgccgcaccg ccgattcggg ggtgagcagg ggcaaaagct cctccgaggc
     2461 ctcggggatc agggcctgcc actcttccag ggataggctc gccaggggcc tctgtcgatc
     2521 caggcaccac cgcaccgccc ggccggtctt ctcgtgggcg gtgcgaaaag gcaccccccg
     2581 gcgcaccaga aactccgcca catccgtgga gaggagatag ccgtcccgga aggcctcccg
     2641 ggcccggggc tcgtccactt ccacccgggc caggagcggg ggcaagacgt ccagcacggc
     2701 ggcggcggta tccagggcgc cccagaggcc ccgcttgtcc tcctggaggt ctcggttgta
     2761 ggtgagcggc aggcccttgg tggtggtcat gaggtccacc aggtgcccca cgaactgtcc
     2821 cgccttgccc cggatgagct ccagcacgtc ggggtttttc ttctggggca tcatgctcga
     2881 ccccgtacag aaggcgtcgg ggaggcggat ccagccgaac tccgtggagg cgtagacgat
     2941 caggtcctcc gccaggcggc tggcgtggat cccgaaggag gcggcggcgt agagggtgtc
     3001 caagagataa tcccgggaag ccacggtgtc catgctgtta tccgtgacgc gggaaaagcc
     3061 cagatcccgg gccacgaagg tccggtccag gggcagggtg gagcccgcca aggcccccga
     3121 gcccaggggg cactcgtcgg cggcgtcgtg ggcggcggaa aagcgcttgt cgtcccggag
     3181 gaaggcccag aaatgggcca tccaatagtg ccccaaggcc acgggctggg cctgctgcaa
     3241 gtgggtatac cccggcacca gcacctcggc gtggctctgg gcccgcccca gcaggacgga
     3301 gagcaagtcg tccaaggcgt cctgcaaccc caagatcgcg cccctcaggt aaagtctcag
     3361 ggcggtggcc acttggtcgt ttcggcttcg ccccatgtgg a
//
LOCUS       DAOOER010000128         3400 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_26289_length_3400_cov_5.843946, whole genome shotgun sequence.
ACCESSION   DAOOER010000128 DAOOER010000000
VERSION     DAOOER010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26289_length_3400_cov_5.843946"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /gene="amrS"
                     /locus_tag="PL162_09595"
     CDS             complement(<1..1022)
                     /gene="amrS"
                     /locus_tag="PL162_09595"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HQF92311.1"
                     /translation="MDSLRSSPALQWEWEGPAVRCLLCPHRCLLSPGKAGRCGVRRHE
                     AGRGLVTLNDGGCAAVAVDPMEKKPLFHFRPGSLVLSLGTVGCNMRCPFCQNWPLARA
                     MDPLEGDVPLNHRHPLPLELLTPQGVRDEARRLRVSSVAFTYNEPMVWFEFLRRTLPV
                     LKEAGLATLLVTNGMINPGPLEELIPFIDGANVDVKAFDGRVYRDTLGGDLTTVCATV
                     EALRRTRIHVELTFLLVPDLNDDRESFSRMLQWISSLPGPIPPPLHLSRSFPQYRWRG
                     PSPTMEELRDFLEEARRILPFVYLGNTDEPEVTKCRRCGRDIVTRRRYTIFHNSLDTS
                     GKCGYCG"
     gene            complement(1063..1635)
                     /gene="amrA"
                     /locus_tag="PL162_09600"
     CDS             complement(1063..1635)
                     /gene="amrA"
                     /locus_tag="PL162_09600"
                     /inference="COORDINATES: protein motif:HMM:NF013988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HQF92312.1"
                     /translation="MERKAPFQASLAACQIARFVRTGRGLSRDEARSCYPDEALWGAP
                     GACFVSIKGSDGSLRGCIGTILPTAPSLGEEILANAVAACSQDPRFLPVTPPELPGLR
                     ISVDALSAPEPISSPEELDPRRYGVIVSRGRARGLLLPDLPGVDSVEEQLRIACAKAG
                     LASLQGVQLARFTVERYDGGEAVDAASAPA"
     gene            complement(1640..2428)
                     /locus_tag="PL162_09605"
     CDS             complement(1640..2428)
                     /locus_tag="PL162_09605"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol dioxygenase subunit B-like
                     domain-containing protein"
                     /protein_id="HQF92313.1"
                     /translation="MWSWACLVPHPPIAVPEVGRGREKEALKTLEGFGALAETLEDKA
                     PDVLVVLSPHQPYAPGVLFVCGERFEGNLESFGAPQVQLQADGATEETEEILGILRDV
                     PVHRVDRETATLDHASLVPLWFLSRRWRSMPRLVVANPVGLELAEALALGRELRSLSG
                     SGSWALVASGDLSHRLIPGAPAGYSPSGRIFDEAVLRSLETSSPAPLERLSPEVLEEA
                     GECGLRSVLVLLGFAGGDPLKTLSYEGPFGVGYATALWTPRQEG"
     gene            complement(2560..>3400)
                     /locus_tag="PL162_09610"
     CDS             complement(2560..>3400)
                     /locus_tag="PL162_09610"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HQF92314.1"
                     /translation="LRGVMGREYARKSGENDRVARAIYEQYLPAAAGDRLPSDILGAL
                     LGVADRMDTVVACHKVGLDPTGSQDPYGLRRAARCMNEIFWGLSLDGDLKELVRVAAQ
                     EIGAGDDVVSRVESFLQARLQVQLKERGYPHDVVTLAMGVAWHRPLHLERLLDTLQGV
                     ASEAWFQALVTAACRVRNILTKAGNPPAGWREELLQEEGERHMAQAVADLSPEVHGAV
                     KDFDWRKLTEALARLEPPVTEFFEKVLVMAEDGAVRSNRLGILASCHALFLTAGDLSR
                     MKK"
BASE COUNT          647 a         1148 c         1023 g          582 t
ORIGIN      
        1 ccgcagtacc cacacttccc tgatgtatct agggaattat ggaatatagt atacctccga
       61 cgggtcacta tgtcacgccc gcaccgacga cattttgtga cttccggctc gtccgtgttg
      121 cccaggtaca cgaagggtaa gatccgtcgc gcttcctcga ggaagtcgcg taactcttcc
      181 atcgtggggg aaggacctcg ccatcggtac tggggaaagg aacgggaaag gtgcaacggc
      241 ggggggatgg ggcccgggag ggaagaaatc cactggagca tgcggctaaa gctttctcgg
      301 tcgtcgttga ggtccgggac gaggagaaag gtgagctcca cgtggatgcg ggtccttcgc
      361 agggcctcca cggtggcgca gacggtggtc aggtctcctc ccaacgtatc ccgataaact
      421 ctcccgtcga aggccttgac gtccacgttg gccccgtcga taaagggaat aagctcttct
      481 agggggccgg ggtttatcat cccgttcgtc accagcagcg tggccaaccc cgcctctttt
      541 aatacaggaa gggtccgccg caggaattca aaccacacca tgggctcgtt gtaggtgaaa
      601 gctacggagg acacccgaag acgtcgggcc tcatctcgca ccccctgggg agtcaggagc
      661 tctaaaggga gggggtgccg gtgattcaaa gggacgtccc cttccagggg atccatggcc
      721 cgggccaagg gccagttctg gcagaagggg cagcgcatgt tgcaccccac ggtccccagg
      781 gataacacca aagatccggg gcgaaagtga aaaaggggct ttttctccat gggatccaca
      841 gccaccgccg cacatccccc gtcatttagc gtgacgagac cccgccctgc ctcgtgacgg
      901 cgtaccccgc accgccccgc cttcccggga gagagaaggc aacggtgagg gcagaggagg
      961 caccgcaccg ccgggccttc ccattcccat tgaagagctg gcgacgaccg gagggaatcc
     1021 atgctttttt gtccttccta agtccctcgg ggaggagaag gcctaggcgg gggcggaggc
     1081 agcgtccacc gcttctcccc cgtcgtagcg ctccacggta aagcgcgcca gttggacccc
     1141 ctggagcgag gccaaccccg ccttggcaca ggcgatgcgc agctgttcct ccacgctgtc
     1201 cacccccggc aggtccggca gcaggagccc ccgggctctc ccccgggaga cgatcacgcc
     1261 gtacctccgg gggtcgagct cttccggcga cgagatgggc tccggcgccg aaagggcgtc
     1321 tacggatatt ctcaagcccg ggagctccgg gggagtcacc ggaagaaatc gggggtcctg
     1381 ggagcaggcc gccaccgcat tggccaggat ctcttccccc agggatgggg ctgtggggag
     1441 gatcgtgccg atacaccccc gaaggcttcc atccgacccc ttgatggaca cgaaacaggc
     1501 ccccggggcc ccccacaggg cctcgtcggg atagcaggac cgcgcttcat ccctcgatag
     1561 cccgcgtccg gtcctcacaa aacgggctat ctgacacgcc gccagagaag cctgaaaggg
     1621 ggccttccgt tccatgggat catccctcct ggcgaggggt ccatagggcc gtggcatatc
     1681 ccacgccgaa ggggccctcg taagagaggg tcttcagcgg gtcccccccc gcaaacccca
     1741 gcaacacgag taccgaacgc aagccgcact cccccgcttc ttctagtacc tctggggaga
     1801 ggcgttccag gggggcgggc gacgaggttt ccagagagcg aagcaccgct tcgtcaaaga
     1861 tccgccccga aggagagtat cccgccggag ccccggggat cagacgatgg gatagatcgc
     1921 cgctggccac cagggcccag gatccactcc cggataggct ccgaagttcc cgacccaagg
     1981 ccagggcctc ggcgagctcc aacccaacgg ggttagccac caccaggcgt ggcatcgacc
     2041 gccaacggcg gctcagaaac cacaggggga cgaggctcgc atggtccagg gtggccgtct
     2101 ctcggtccac ccggtgcacc ggcacatccc gcaggatccc cagaatttcc tccgtctcct
     2161 ccgtggcgcc gtcggcttgc aactgaacct ggggtgcccc gaaggattcc aagtttcctt
     2221 cgaagcgttc cccgcataca aatagcaccc ccggcgcata gggttgatga ggggagagca
     2281 ccaccagcac gtccggtgct ttgtcttcaa gggtttccgc gagggctcca aagccctcca
     2341 gagttttcaa ggcctctttc tcccgccccc gtcccacctc cgggaccgct atggggggat
     2401 ggggcacgag acacgcccac gaccacatgg ccgttcctcc ttcctaaaaa cacggatacc
     2461 gggaccaccc tgcctattgt gccacgtttc gacgactcgc gatagggcgt tttcgtctcg
     2521 ccgccatagg tctgtaatcc ccatcgccac tgtctatgac tattttttca tgcgagacag
     2581 gtcccccgcc gtcaggaaca gggcgtggca ggaggccaaa atccccagcc ggttactcct
     2641 cacggcccca tcttcggcca tgaccaacac tttctcgaaa aactccgtca ccggcggttc
     2701 caacctggcc agggcctccg tcaatttgcg ccagtcgaaa tccttcacgg ccccgtggac
     2761 ctcgggagac aggtcggcca ccgcctgggc catgtgccgt tctccctctt cctggaggag
     2821 ctcttctcgc catcccgccg gggggtttcc tgccttggtc aaaatgtttc tcacgcgaca
     2881 agccgccgtg accagggcct ggaaccaagc ctcggaagcc accccctgga gggtgtctag
     2941 gagcctttcc aggtgaaggg ggcgatgcca agccactccc atggccaggg tcaccacgtc
     3001 gtggggatac ccccgctcct tgagctggac ttggaggcgg gcctgaagga agctctccac
     3061 ccgggacacc acgtcgtcgc ccgcgccaat ttcttgggcc gccacgcgca ccagttcttt
     3121 caagtcccca tccagggaga gcccccaaaa gatctcattc atgcaacggg ccgcccggcg
     3181 aaggccgtag gggtcctgag aaccggtggg atctagtccc actttatggc aggccaccac
     3241 ggtgtccatg cggtcggcca ctcccagcag ggcccccaag atgtcggagg gcagccggtc
     3301 tcccgccgcc gccgggaggt attgttcgta gatagcccgg gccacccggt cgttttcccc
     3361 gctcttacgg gcgtactccc tccccatgac ccctcggagc
//
LOCUS       DAOOER010000129         3395 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_26344_length_3395_cov_9.286527, whole genome shotgun sequence.
ACCESSION   DAOOER010000129 DAOOER010000000
VERSION     DAOOER010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26344_length_3395_cov_9.286527"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PL162_09615"
     CDS             <1..539
                     /locus_tag="PL162_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HQF92315.1"
                     /translation="RDRRNGAICDKLGHLGVPVSLEEALRESRGEVLGRPHIAQAMIR
                     KGYARDMKDAFCRYLGRGAPAYVPRVRLCPERCLELVANAGGVTVLAHPAQTNLPPDD
                     LEALLRRLRDRGLWGLECHIPASPPDEIFFHLRLAGKLGLHATAGSDFHGSHRPGVDL
                     GCGVEESLLPWARLGVAL"
     gene            685..1716
                     /gene="ltaE"
                     /locus_tag="PL162_09620"
     CDS             685..1716
                     /gene="ltaE"
                     /locus_tag="PL162_09620"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HQF92316.1"
                     /translation="MAIDLRSDTVTQPHASMRAAMAAAEVGDDIYGDDPTMRRLEEQA
                     AALVGHEASLFATSGTQGNLVALLTHCGRGDGVILGAESHIYYYEKGGMALGGLFPLV
                     VSDEGGLPCLEGVAAWCRDDANVHFVPGRLLCLENTHNRHGGVAIPPGPFGDAARFAR
                     DRGLRTHLDGARIFNAAAFHGVDVRAYTTEVDSVQFCLSKGLGAPVGSMLCGNRAFID
                     RARHWRKALGGGMRQSGVLAAAGIVALDTMIDRLGEDHENARMMASLLQEGGLAVCPN
                     PYPTNMVFVELPGDGSRTEEILRRCRERGVLFGTMGPRVARFVTHGDVSRDEVRAAAA
                     IVREEVGRG"
     gene            1713..3080
                     /locus_tag="PL162_09625"
     CDS             1713..3080
                     /locus_tag="PL162_09625"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HQF92317.1"
                     /translation="MRSASLPRAPEVESWAADCLAQAVAQGATQADLLFTAGAGTSLV
                     LRDGEEEERSRGVSCGVGLRTILGDGRQGVAFINGFDPAMRSCLVPWSLANARDSTAD
                     EAVGLYEGPLGECDALEMEDPALEALSPADRFARAVEITDRARGEDPRVVSVRAASWY
                     DGWGKLFYASSAGLAGWQRSTSVGCGLSVVLQEGDAFEMGGSGEQARFMADLSLEKMV
                     REGVRRTALSLGGDPVPTGRYSLVLDPESAASLVDEVAELFLGSNVQKGKSLFRGRLG
                     EEVGEAVLSLVDDGRLPRRDGSAPFDGEGFPTGRTLLLDRGRVNAFLYDLATARREGG
                     RSTGNAVRGISSVPDVGTSNVFILPGDEAPEAIRRQRDIALLVTEFMGLHTLDPVSGE
                     FSLGAKGAVLRSGLPAEPFAGVTVAGNLTDLLRGIVAVGNDLRFFGSTGGCTILVDRV
                     AVAGR"
     gene            3134..>3395
                     /locus_tag="PL162_09630"
     CDS             3134..>3395
                     /locus_tag="PL162_09630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92318.1"
                     /translation="MARGKRSVALLGGIVLLAGVFGVWGEKSGARGAEAWQLWRGNAL
                     LGAVEVKDTPGGTLVPLDALASSAGYSGGPGEEVYLMEQGGRR"
BASE COUNT          505 a         1096 c         1253 g          541 t
ORIGIN      
        1 accgagatcg gcgcaacggg gccatctgcg acaagctcgg tcacctcggc gtccccgtct
       61 ccctggaaga ggccctgcgg gagtcccggg gggaagtcct ggggcggccc cacatcgccc
      121 aagccatgat ccgcaaggga tacgcccggg acatgaagga cgccttctgc cgttacctgg
      181 ggcgaggggc tccggcttac gtgccccggg tccgcctctg tccggagagg tgcctggagc
      241 tcgtggcgaa cgccggagga gtgacggttt tggcccatcc ggcccagacg aacctgcccc
      301 ccgacgacct ggaggccctc ctgagacgcc tccgggaccg ggggctctgg ggcctggagt
      361 gccacatccc cgcctctccc ccggacgaga ttttcttcca cctccggttg gcgggaaagc
      421 tgggactcca cgccaccgcc ggatcggact tccacggaag ccatcgcccc ggggtggacc
      481 tgggatgcgg ggtggaagaa tccctccttc cctgggcccg actgggcgtg gcgctgtgac
      541 ccggcgaggg gacaaaaaaa ggcttccctt tcccggggag gccttcttgt cgtccccgga
      601 aaggtccggg gccaaggcgt gaaccccaag ggttcgcggg aaggccccca tggtactctc
      661 aaaaaggagg tttaacttaa agacatggcc atcgaccttc gcagcgacac ggtgacccaa
      721 ccccatgcct cgatgcgcgc cgccatggcc gccgccgagg tgggagacga catctacggc
      781 gacgacccca ccatgcgccg cctggaagaa caggcggcgg ccctggtggg gcacgaagct
      841 tccctctttg ccacgtcggg aacccagggg aacctggtgg ccctcttgac ccactgcggt
      901 cggggggacg gggtcatcct gggagccgag agccacatct actactacga gaagggtggc
      961 atggccctgg ggggactatt tcccctggtg gtctccgacg aaggagggct tccctgcctc
     1021 gaaggcgtgg cggcctggtg ccgggacgat gccaacgtcc acttcgtgcc ggggcgcctc
     1081 ctctgtctgg agaacaccca taaccgccac ggaggggtgg cgatcccccc ggggcccttc
     1141 ggggacgcgg cgcgatttgc ccgggatcgg gggctccgga cccatctgga tggggcccgg
     1201 atcttcaacg ccgccgcctt tcacggcgtg gacgtgcggg cctatacgac ggaggtggat
     1261 tcggtgcagt tctgcctctc caaggggctg ggggctccgg tgggatccat gctctgcggg
     1321 aaccgggcct tcatcgaccg ggcccggcac tggcgcaagg ccttgggggg cggtatgcgc
     1381 cagtcggggg tgttggcggc ggcggggatc gtggccctcg acaccatgat tgaccgcctg
     1441 ggggaagacc acgaaaacgc ccgaatgatg gcctccctgt tgcaggaggg agggcttgcc
     1501 gtctgtccca acccctaccc caccaacatg gtcttcgtgg agctccccgg agatggatcc
     1561 cggacggagg aaatcctcag aagatgccgg gaacgggggg tgctcttcgg caccatgggc
     1621 ccccgcgtgg cgcgcttcgt gacccacggg gacgtctccc gggacgaagt ccgcgccgcc
     1681 gccgccatcg tgcgggagga agtggggcga gggtgaggtc ggcgagcctt cctcgggccc
     1741 cggaggtgga atcctgggcc gcagactgcc tggcccaggc ggtggcccaa ggggcgacgc
     1801 aggccgacct gctcttcacc gccggggcgg ggacttcctt ggtgttgcgg gacggcgagg
     1861 aggaagaacg ttcccggggg gtctcctgcg gcgtgggatt gcgcaccatc ctgggggacg
     1921 ggcgccaggg agtggccttc atcaacggct tcgaccccgc catgcgctcc tgtctggtgc
     1981 cctggagcct ggccaatgcc cgggatagca cggcggacga agcggtgggc ctctatgaag
     2041 gccccctggg ggagtgtgac gccttggaaa tggaggaccc ggccctggag gccctttctc
     2101 ccgccgaccg ctttgcccgg gccgtagaga tcaccgaccg ggcccggggt gaagaccccc
     2161 gggtggtctc cgtgcgggcc gcttcctggt atgacggctg gggaaagctc ttttacgcct
     2221 ccagtgcggg actggcggga tggcagcgct ccacctcggt ggggtgcggc ctctccgtgg
     2281 tgctccagga gggggacgcc ttcgagatgg ggggatcggg ggagcaggct cgcttcatgg
     2341 cggacctatc cctggagaag atggtccggg aaggggtgcg acgcacggcc ctctccctgg
     2401 ggggggatcc ggtgcccacg gggcgctatt ccctggtgtt ggatcccgaa agcgccgcat
     2461 ccctggtgga cgaagtggcg gagctcttcc tggggtccaa cgtgcagaag gggaaatccc
     2521 tcttccgggg aaggctgggg gaagaggtgg gagaagcggt gctctccctg gtggacgacg
     2581 gccgcctccc ccggcgcgac ggatcggccc ccttcgacgg cgagggcttc cccacggggc
     2641 ggaccctcct gctcgaccgg gggagggtga acgccttcct ctacgacctc gccacagccc
     2701 ggcgggaggg ggggcgctcc acgggcaacg ccgtgcgggg gatctcctcc gtccccgacg
     2761 tggggacctc caacgtcttc atcctccccg gcgacgaggc ccccgaggcg atccggcgcc
     2821 agcgcgacat cgccctcctg gtgacggaat tcatggggct tcacaccctc gaccccgtga
     2881 gcggcgagtt ctccctgggg gccaagggag ccgtcttgcg gtcgggcctc cccgccgagc
     2941 cctttgccgg agtgacggtg gcgggtaacc tgacggacct cctccggggc atcgtcgccg
     3001 tgggcaacga cctgcgattt ttcggcagca ccggcggatg caccatcctg gtggatcggg
     3061 tggcggtggc gggtcggtga gagccctcgg gcctcggagg ccttcaacct tgaattctgg
     3121 cggtgatctt ggcatggcgc gaggcaaaag atcggtggcc ctcctggggg ggatcgtcct
     3181 tctggcgggg gtctttggtg tgtggggaga aaagtcgggc gcccggggcg cggaggcctg
     3241 gcagctctgg agggggaacg ccctcttggg ggccgtggag gtcaaagaca ccccgggggg
     3301 aaccctcgtg cccctcgacg ccctggcgtc gtcggcggga tactccgggg gccccggaga
     3361 agaagtttac ctgatggagc agggcggacg ccggg
//
LOCUS       DAOOER010000130         3377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_26543_length_3377_cov_8.394040, whole genome shotgun sequence.
ACCESSION   DAOOER010000130 DAOOER010000000
VERSION     DAOOER010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26543_length_3377_cov_8.394040"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..626)
                     /locus_tag="PL162_09635"
     CDS             complement(18..626)
                     /locus_tag="PL162_09635"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQF92319.1"
                     /translation="MTRRMLLVRHGDPRLPAEGKVFLGSRSDVPLAPEGISRMERLRE
                     HLENFPLDRIVTSPLRRARESATLLAEGRSLAVEVDPALREIDLGEWDGLPFATVRAR
                     DPEGFAERGRRLADFRPPGGESFRDLAARVAPHLTHLLATSSGNLLMVAHAGVFHAMI
                     CTLLNLPLERLFLLTQSYGGVHLLDDLRGEWRVKRLNWSPEL"
     gene            complement(623..1789)
                     /locus_tag="PL162_09640"
     CDS             complement(623..1789)
                     /locus_tag="PL162_09640"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HQF92320.1"
                     /translation="MTDHSPRPTHTASPNTMPHKTPAALVLAAGRSSRMGCGKMLLPL
                     GGSTVLAQAVTRLREGGAHPVVVVTGHHREAVEGEARQLGCPTVANPDPEGDMFSSLR
                     VGLSALPPKTRAVLVLPGDTPLVRPSTCRALTAALQNADAVIPTFLQRGGHPVLLGRA
                     ALASLATRPAPEGLRSVLSAPDLRVHRVPVPDSAILRDADTPEDFAALTAYAAREEIP
                     TGEEAEALLDLHHTPREVRRHAQATARAARALGEALTRGGHALDLPLLEAAALLHDIA
                     KGSRRHAEAGGSFLASWGFAAVGAVVACHMDLDAPAPSREAEVLYLADKMLDGETLCA
                     LEERRRRVTLRFREDPKALRGALARLDRARELEQRLNALLPAPVCSIFAEALAS"
     gene            complement(1923..2492)
                     /locus_tag="PL162_09645"
     CDS             complement(1923..2492)
                     /locus_tag="PL162_09645"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HQF92321.1"
                     /translation="MSDLTGDRVRLRAYRESDIPALREWVNDPQVTRFLEGLSLFPHT
                     RHDTESFVRAQLAPRPLHPESLELVVADRHTDVYLGQVGLLTIDWPNRATALGIVFPR
                     KNQGKGYGGEALDLLGRLVFDDLGLHRLELEVHEDNLPALRCYQRCGFREEGRRRERR
                     FSGGRFRDVVIMALLESDHRLRQNGTTSS"
     gene            complement(2497..3057)
                     /locus_tag="PL162_09650"
     CDS             complement(2497..3057)
                     /locus_tag="PL162_09650"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HQF92322.1"
                     /translation="MPHILGQRILLREYRLSDLPEMRKWVNDPLVTKGMSDIFLFPHS
                     EEATEKFLRDVLEDRTESREFVVASRPSEDFLGQIGLKVLDWKNRFAEISLVFPREHQ
                     GQGYGTEALGLLQRFAFEDMGLNRLELFVHEDNAPALRCYQKCGFREEGRFRQRFFRQ
                     GRFWDMILMALLAEEYRSFGPPPEAE"
BASE COUNT          576 a         1082 c         1200 g          519 t
ORIGIN      
        1 cccttccggc gggtcgatca gagctcgggg ctccagttga gccgtttgac gcgccactct
       61 ccccggaggt cgtccagaag gtggacgccg ccgtagcttt gggtcaggag aaagaggcgt
      121 tccagaggga ggttgagcag ggtgcagatc atggcgtgaa agacccccgc atgggccacc
      181 atcaggagat tgcccgaaga ggtggcgagg agatgggtca gatggggagc cacccgggcc
      241 gccagatcgc ggaagctctc tcctcccgga gggcgaaagt ccgccagacg acggccccgc
      301 tccgcgaagc cctcgggatc ccgggcccgc acggtggcga aggggaggcc gtcccactcc
      361 cccaggtcga tttcccgcag cgccggatcc acctccaccg ccagggaccg cccctcggcc
      421 agcagggtgg cgctttcccg ggcccggcgc agggggcttg tcacgatgcg gtccagggga
      481 aagttctcca gatgttcccg gagccgttcc atgcgggaaa tgccttcggg ggccaggggc
      541 acgtcggagc ggctccccag aaaaaccttg ccttcggcgg gaaggcgggg atccccgtgg
      601 cgcaccaaca acatgcgccg ggtcatgacg ccagggcctc ggcgaagatg gagcataccg
      661 gcgcgggcaa gagggcgttc aggcgttgtt ccagctcccg ggcccggtcc aggcgtgcca
      721 gggccccccg gagggccttg gggtcctccc gaaagcgtag cgtgacccgc cgtcgccgct
      781 cttccagggc gcagagggtt tccccgtcga gcatcttgtc cgccagatag agcacctcgg
      841 cctcccggga gggggccggg gcgtcgagat ccatgtgaca cgccaccacc gctcccacgg
      901 cggcaaagcc ccaggaggca agaaagctcc cccccgcttc ggcgtggcgc cgggaaccct
      961 tggcgatgtc gtgcagcagg gcggcggctt ccagcagagg aagatccagc gcgtgccctc
     1021 cccgggtcag ggcttcgccg agggcccggg cggcccgggc cgtggcctgg gcgtggcgcc
     1081 ggacctcccg gggggtgtgg tgaaggtcta aaagggcctc cgcctcctcg ccggtgggaa
     1141 tctcttcccg ggcggcgtag gccgtgaggg cggcgaagtc ctccggggtg tcggcgtccc
     1201 ggagaatggc gctgtcgggg acgggaacgc ggtgaacccg gaggtccggg gcggagagca
     1261 cgctccgcaa tccttcgggg gcggggcggg tggccaggga ggcgagggcg gcgcgcccca
     1321 ggagcacggg gtgacccccc cgctgcaaga aggtagggat caccgcgtcg gcgttctgga
     1381 gggcggcggt gagggcccgg catgtggagg ggcgcaccag gggcgtgtct ccggggagca
     1441 ccagcactgc ccgggttttg ggaggtaggg cggaaagccc cacccggagg gagctgaaca
     1501 tgtccccctc ggggtccgga ttggccacgg tggggcatcc gagttgccgg gcctctcctt
     1561 ccaccgcttc ccggtggtgt cccgtcacca ccaccacggg gtgcgctccc ccctcccgga
     1621 gccgggtcac cgcctgggcc agcacggtgg aaccccccag gggaagaagc atcttgccgc
     1681 accccatccg ggaagagcgc ccggcggcga ggaccagggc ggcgggagtt ttgtggggca
     1741 tggtattggg ggacgcggtg tgggtcggac gtggggaatg atcggtcatg gatggaggtc
     1801 ctcctttaag gaaaaaacgt ttccgtcgcc gggggcgcct cgggcccggc ggcctcgtga
     1861 gctcccgccc caggacgagg gcaggagcac cccggggtga gcgtccgagg gcgccgagat
     1921 cgttacgagg aggtggtgcc gttttgccgc aaacggtggt cggattccag cagggccatg
     1981 atgaccacgt cccgaaagcg tccgccggaa aagcgccgtt cccggcgtcg cccctcttcc
     2041 cggaaaccgc acctttggta gcaccggagg gcggggaggt tgtcctcgtg aacttcgagc
     2101 tccaaccgat ggagccccag gtcatcgaac accagccgcc ccagaaggtc cagcgcctcg
     2161 cccccgtacc ccttgccctg gttctttcgg ggaaagacga tgcccaacgc cgtggcccgg
     2221 ttgggccagt cgatggtgag gagccccacc tgccccagat agacgtcggt gtggcgatcc
     2281 gccaccacca gctccaggga ttcaggatgg agggggcggg gggcgagctg ggcccggaca
     2341 aaactttcgg tgtcgtgccg ggtgtgggga aaaagggaga gcccctccag aaagcgcgtc
     2401 acctggggat cgttcaccca ctcccgcagg gcggggatgt ccgactcccg gtaggcccga
     2461 agccgcaccc gatcgcccgt gaggtccgac accgggttac tccgcttccg gggggggccc
     2521 gaaagatcgg tactcttcgg ccaggagggc catgaggatc atgtcccaaa agcgcccctg
     2581 gcggaaaaag cgctgtcgga agcgcccctc ctcccggaag ccgcactttt ggtagcaccg
     2641 gagggcgggg gcgttgtcct cgtggacaaa gagctccaga cggtttagcc ccatgtcctc
     2701 aaaggcaaag cgctggagca gaccgagggc ctcggtgccg tacccctgcc cctggtgttc
     2761 ccggggaaag accagagaga tctcggcgaa gcggttcttc cagtcgagga ctttcagccc
     2821 gatctgcccg agaaagtcct ccgaggggcg agatgccacc acgaattccc gggactcggt
     2881 gcgatcctcc agcacgtcgc gaagaaattt ttccgtggct tcttcggagt gaggaaagag
     2941 aaagatgtcc gacatgccct tggtgaccag gggatcgttc acccacttcc gcatctccgg
     3001 cagatcggaa aggcggtatt cccggagaag aatccgttgt cccagaatgt gcggcatgaa
     3061 aataactcct ttctcaaaaa gaaaacttcg gaacgggagg tctcaaaaaa acccttgcca
     3121 tgaccgggaa aaaaggccga aactccggct tttggcgggg tcatgtcggg gcgaaggatt
     3181 tttcgccccg acggcgttgt cgtgatggcg cgggggctga aaaagcccct ccccggtttt
     3241 tggaagctac atcccctcgg cgtctttgcg ccgctccgcc tttttccggg cctcggcgtt
     3301 caggatgccc ttgcgcaccc ggatggactg gggggtgatc tccaccagct cgtcgtcggc
     3361 gatccactcc agcgccg
//
LOCUS       DAOOER010000131         3251 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_27943_length_3251_cov_7.176471, whole genome shotgun sequence.
ACCESSION   DAOOER010000131 DAOOER010000000
VERSION     DAOOER010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27943_length_3251_cov_7.176471"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="PL162_09655"
     CDS             complement(<1..348)
                     /locus_tag="PL162_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQF92323.1"
                     /translation="MLKVSNLWAGYDEVSVLHDVTLEVHPGEVVALVGANGAGKSTMM
                     RCLAGLLKPTGGSIQFLGEEIGGLPAAQVVARGLAYVPEGRRLFGKLSVRENLLLGAY
                     LERRDHRVAEQLGM"
     gene            complement(341..1087)
                     /locus_tag="PL162_09660"
     CDS             complement(341..1087)
                     /locus_tag="PL162_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQF92324.1"
                     /translation="MALLEVQGVSMAFGALMANADVSFSVEEGTIVGLIGPNGAGKTT
                     LFNCISGTYRPTEGHIFFDGQEITGRPPYDICRRGAVRTFQVVRPLKEMSVLENVMVG
                     AYCRVESPEGARKVAEETLALCGMEAKGGLRAGKLTIADKKRLEVARALATKPRLLML
                     DEVMAGLTPSEIKSAVELLLSLRQRGVTLLVVEHIMEAVMPVADKVVVLDGGVKIAED
                     VPRRVVEDERVIAAYLGPKFASKHKEMAHA"
     gene            complement(1093..2058)
                     /locus_tag="PL162_09665"
     CDS             complement(1093..2058)
                     /locus_tag="PL162_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQF92325.1"
                     /translation="MTRINIGWYGFLGVLALLPTALPGAFTNGFNVHVMILVLLYASM
                     AQAWNLIGGYGGQVSFGHSVFFGLGAYGAAMALVTYHGSPWVGALVGSLVAAGAAVII
                     SYPCFRLKGHYFAIATFAIVEIFNRLFMMWNWVGGALGLDYPLLEEGWKNFMWFDTKV
                     PYYYGALGLFAVVFGVVRWVDGHRLGAWLRAVREGQEAAESLGVNSARVKLSALALSA
                     FLAGLCGAFFAQYNYRVDPPMVMSLDMAMKFVLISILGGVGTFWGPLVGAAILMPLQE
                     YTRAAWGGMGGGVDLIIFGVLIIFVVTRQPQGVVGFIGHVFRRKG"
     gene            complement(2063..2923)
                     /locus_tag="PL162_09670"
     CDS             complement(2063..2923)
                     /locus_tag="PL162_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HQF92326.1"
                     /translation="MALFLQVALDGLLNGAVYALVAAGLSLIWGVMDVINFAHGEFLM
                     AAMYVTYWLGFLWHLDPLVSWGASGAFAFLLGVLAYKLVVKRTIGRSAMAALLGTFGL
                     SMVIKNVCLNRFSPNFRLLPDFLLKGKTILLGDLIVPLPQLVTGLFALAVVAALGMML
                     KRTRFGWAVQATAMDREAAELMGIDTEKIYALVFGIGGACVGIAGGLMPSYLAVHPEV
                     GGLFSLIAFVCVALGGFGSIGGALAAALLIGLVEALSGFYLAPVVKYVAVFGLYLAVV
                     FFRPKGLFGW"
BASE COUNT          651 a         1144 c          956 g          500 t
ORIGIN      
        1 catccccagt tgctccgcca cccggtggtc ccggcgctcc agataggccc ccagcaggag
       61 gttttcccgc accgagagct tgccaaagag tctcctcccc tcgggcacgt acgccagtcc
      121 ccgggccacc acctgggctg cggggagccc cccgatctct tcccccaaga actggatgct
      181 cccccccgtg ggtttgagca gccccgccag gcatcgcatc atcgtgctct tgcccgcgcc
      241 gttggccccc accagggcca ccacttcccc ggggtggacc tccagggtca cgtcgtggag
      301 caccgacacc tcgtcgtacc ccgcccacaa attcgacacc ttaagcatgg gccatctcct
      361 tgtgtttgct ggcgaacttg ggtcccaagt aggcggcgat gacccgctcg tcctccacca
      421 cccgtcgggg gacgtcttcg gcgatcttca ccccgccgtc caggaccacc accttgtcgg
      481 caacgggcat caccgcctcc atgatgtgct ccaccaccag gagcgtcacc cctcgctggc
      541 gcagggagag caaaagctcc accgccgact ttatctcgct gggggtgagt cccgccatga
      601 cctcgtccag catgaggagc cggggtttgg tggccagggc ccgggccacc tccagacgct
      661 tcttgtcggc gatggtgagc tttcccgccc gaaggccccc tttggcctcc atgccgcaga
      721 gggctagggt ttcttcggcc accttccggg ccccttcggg gctctccacc cggcagtacg
      781 ctcccaccat gacgttttcc agcaccgaca tttccttcaa aggacgcacc acctgaaagg
      841 tgcgcaccgc cccccgacgg cagatgtcgt agggagggcg ccccgtgatc tcctggccgt
      901 cgaagaagat gtgtccctcg gtggggcggt aggtgccgct gatgcagttg aacagggtgg
      961 tcttgcccgc tccgttgggg ccgatgagcc ccacgatggt cccttcttcc acggagaagg
     1021 agacgtcggc gttggccatg agggccccga aggccatgga aacgccttgt acttccaaaa
     1081 gcgccatgga cctcacccct ttctccggaa aacgtggccg ataaagccca ccacgccctg
     1141 gggttggcgg gtcaccacga agatgatgag gacgccgaag atgatgagat ccacgccccc
     1201 tcccatgcct ccccaggccg cccgggtgta ctcctgcagg ggcatgagga tggccgcccc
     1261 caccaggggc ccccaaaagg tccccacgcc cccgaggatg gagatgagga cgaacttcat
     1321 ggccatgtcc agggacatga ccatgggggg atccacccgg tagttgtact gggcgaagaa
     1381 ggcgccgcag agccccgcca gaaaggccga aagggccagg gccgaaagct tcacccgggc
     1441 gctgttcacc cccagggatt ccgccgcctc ctgcccctcg cgcacggccc ggagccaggc
     1501 tcccagccga tggccgtcga cccatcgcac cacgccgaag accaccgcaa agagtcccag
     1561 ggcaccatag tagtacggca ccttggtgtc aaaccacatg aagttcttcc atccttcctc
     1621 cagcaggggg taatccaatc ccagggcgcc ccccacccag ttccacatca tgaacaatct
     1681 gttgaaaatc tccacgatgg caaaggtggc gatggcaaag tagtgtccct tgaggcggaa
     1741 acagggatag cttatgatca ccgccgcccc cgccgccacc agcgatccca ccagggcgcc
     1801 cacccagggg gagccgtggt acgtcaccaa ggccatggcg gccccgtagg cccccaggcc
     1861 gaagaagacg gagtggccga aagacacctg ccccccgtag cctccgatga ggttccaggc
     1921 ctgggccatg ctggcgtaga gcaatacgag gatcatcacg tggacattga agccgttggt
     1981 aaaggccccg ggtagcgccg tgggaagcag agccaggacc cccaggaagc cgtaccatcc
     2041 gatatttatc cgggtcatgg ggtcaccacc cgaagaggcc cttgggccgg aagaacacca
     2101 cggcgaggta gaggccgaag accgccacat acttcaccac gggggcgaga taaaatccgc
     2161 tgagggcttc caccaggcca atgaggaggg ccgccgccag ggccccgccg atgctaccga
     2221 agccccccag ggccacgcag acgaaggcaa tcaggctgaa aagccccccc acctccggat
     2281 ggaccgccag gtaggagggc atgagccccc ccgcgatgcc cacacaggcc ccaccaatgc
     2341 cgaacaccag ggcgtagatc ttctcggtgt cgatgcccat gagctccgcc gcctcccgat
     2401 ccatcgccgt ggcctgcacc gcccacccga atcgggtgcg tttgagcatc atccccaggg
     2461 cggccaccac ggccaaggca aaaaggcccg tcaccagctg gggcagggga acgatgagat
     2521 ctcccaagag gatggtctta cccttcagta aaaaatcggg cagcagacga aagtttgggg
     2581 agaagcggtt caaacacacg ttttttatca ccatggatag gccaaaggtg ccgaggagcg
     2641 ccgccatggc ggaccgacct atggtgcgct tcaccaccag cttgtacgcc aggactccca
     2701 ggagaaaggc gaaggccccc gaggcccccc aggaaaccag gggatccagg tgccatagaa
     2761 atcccaacca gtaggtgacg tacatggccg ccatgaggaa ctccccatgg gcgaagttga
     2821 tgacatccat gaccccccag atgagggaaa ggcccgccgc caccagggcg tacaccgccc
     2881 cgttcagcag gccatccagg gccacttgca gaaagagcgc catccatctc tcaccccttt
     2941 tcgctttagg aaacgggccg cccataatcc ggcgaagaca ccccgacggg gggcggcggg
     3001 aacgatgcct ggcgcacctc cccgccgccc ccctgcggtg cgatggtccc gcttctagga
     3061 ggtcctccgg aaggcccttc cggaggccag attcatgcgg aacgcctctt ccatgcctag
     3121 cgcttgtccc agggagtcat ggggaacacc acaggggtgt ccgccaggtt ctccgggaag
     3181 acgcgcttgt aggatccccc ctggagctgg gtgatcatgc tcttggcctg gacgttctgc
     3241 cctccggaga c
//
LOCUS       DAOOER010000132         3146 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_29176_length_3146_cov_7.799418, whole genome shotgun sequence.
ACCESSION   DAOOER010000132 DAOOER010000000
VERSION     DAOOER010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29176_length_3146_cov_7.799418"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            77..1450
                     /gene="trkA"
                     /locus_tag="PL162_09675"
     CDS             77..1450
                     /gene="trkA"
                     /locus_tag="PL162_09675"
                     /inference="COORDINATES: protein motif:HMM:NF007031.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HQF92327.1"
                     /translation="MTGMRVVVIGAGEVGFPVASKLASEGHDVTVVERDEDLAAKVED
                     ELDVMVVRGNGARPQVLERAGVAPGLRTDLLIACTDRDEVNILACWIARRAGVEKVMG
                     RARSLEFTDTDTWARELDIDMLISPERSAAREIEELLLVASAVRTVEIASGKAALYAF
                     RVAEDASMQGVSMAELRSRYSGLSAINVYVDREGGGFVPSGDSVLEPGDLCYVVTFRD
                     QVGFLETLFDPRKRRPLHRVLVVGGGKIGYQVAHRLERRSRSIDIRLIDRDREKCDRI
                     ARELGRTVVLAGDGTDRDLLEYEGVREAEGFVTTTSSDESNILIGVLGKSLGAAKAVA
                     VVRRNAFMQMHAHLDIDAMVNPHMAMASVIMQHVKFPRASGHLSVLESIGAEMMEGTI
                     PDQGRAAGCAVRDIGLPRGIILALVERGTEAFVPWGDTVLQGGDRVILFSSSELMSRA
                     VELLEVR"
     gene            1453..2904
                     /locus_tag="PL162_09680"
     CDS             1453..2904
                     /locus_tag="PL162_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HQF92328.1"
                     /translation="MHRAVVCKTLSLVTAVVTVFQFFPMLWSIRDATPDLNPFLAALG
                     AGAALSALLFVAGRDADPRDMGPREALTVVTLAWVCASGVGALPFWISGAVPTYTDAF
                     FEAMSGFTTTGASVLTHIEGNPRGILFWRSLTHWLGGMGIIVLSLAVLPMLGVGGTEL
                     LKAEAPGPIKEKLTPRVQQTALILWGIYVLFTVVQTGALLAGGLSFFDALTHTFGTVA
                     TGGFSPLNQSVGQYHSPYVEWVITLFMFLSGASFALHYRFLRGDGSAHWRDPEFCLYT
                     GITVGATALVTGALLASGVCDGLSDALRYAAFQVVSVLTTTGFVTADFEQWPYVTQFV
                     LLLLMFIGGCAGSTGGGMKNIRVLLVWRQARSELLRLVHPRGIFPVRVGGAPVERSVV
                     TSVTAFFTLYVISFALSSLALTGLGMDVLSATTGVAACLGNVGPGLGSVGPMDNFSGV
                     PLLGKWVLSFDMLLGRLEIYTVLLIFLRGAWRG"
BASE COUNT          475 a          911 c         1124 g          636 t
ORIGIN      
        1 ggaggccgaa aggcgggctc tgtgatatca tggcccccgg agcgaagggc tccggaagag
       61 ttgaactttt acggagatga caggcatgag ggtcgtggtg atcggggcgg gggaagtggg
      121 ttttcccgtg gccagtaagc tggcgtcgga gggacatgac gtcacggtgg tggagcgaga
      181 cgaagatctg gccgccaagg tggaagacga gctggacgtc atggtggtgc ggggcaatgg
      241 ggcccggcct caagtgttgg aaagagccgg ggtggccccg ggtttgcgta cggaccttct
      301 catcgcctgc accgataggg acgaggtcaa catcctggcc tgctggatcg cccgtcgggc
      361 cggggtggag aaggtcatgg ggcgagcccg gagcctggag ttcacggaca ccgacacctg
      421 ggcccgggag cttgatatcg acatgctcat ctcccccgag cgctccgccg cccgggagat
      481 agaagagctt ctcttggtgg cctcggcggt gcgtacggtg gagatcgcca gcggcaaggc
      541 ggcgctctac gcctttcggg ttgccgagga tgcctccatg caaggggttt ccatggcgga
      601 gctgcggtcc cgatactcgg ggctttcggc catcaatgtc tacgtggatc gggaaggggg
      661 agggtttgtc ccctcggggg attcggtgct ggagcccgga gacctctgtt acgtggtgac
      721 ttttcgggac caggtgggtt ttttggagac cctctttgac ccccggaagc gccgccccct
      781 ccacagggtg ctggtggtgg ggggagggaa gatcggatac caggtggccc accggctgga
      841 gcgccgctct cggtccatcg acatccgcct catcgaccga gaccgggaaa agtgcgaccg
      901 catcgcccgg gagctggggc gcaccgtcgt tttggccgga gacggcaccg accgggatct
      961 attggaatat gagggagtcc gcgaggctga gggcttcgtc accaccacgt ccagcgacga
     1021 atcaaacatc ctcatcgggg tcctgggcaa gtccctgggg gccgccaagg ccgtggcggt
     1081 ggtgcgccgg aacgccttca tgcagatgca cgcccacctg gatatcgacg ccatggtgaa
     1141 cccccacatg gccatggcct cggtgattat gcagcacgta aaattccccc gggcctcggg
     1201 tcacctttcg gtgctggaga gcatcggcgc cgagatgatg gaagggacca tcccagatca
     1261 gggacgggcg gcggggtgcg ccgtgagaga cattggctta cctcggggca tcatcctggc
     1321 cctggtggag cgggggacgg aggcctttgt cccctggggg gacacggtgc tccagggggg
     1381 agatcgggtg atcctcttct cttcgtcgga gctcatgtct cgggcggtgg aattgctgga
     1441 ggtgcgttag tcgtgcatcg agccgtcgtg tgcaagaccc tttctctagt gacggcggtg
     1501 gtgacggtct ttcagttttt ccccatgcta tggtccatcc gcgacgccac gccggaccta
     1561 aacccctttc tggcggccct gggggcgggg gcggcccttt cggccctgct ttttgtcgcc
     1621 gggcgagacg ccgatcctcg ggacatgggc ccccgagagg ccctgacggt ggtgactttg
     1681 gcttgggtct gcgcctcggg ggtgggggcc ctcccctttt ggatcagcgg cgcagtgccc
     1741 acttataccg acgcgttctt cgaggccatg tcgggcttta ccaccacggg ggcctcggtg
     1801 ttgacccaca tcgagggaaa cccccggggg attttgttct ggcgcagctt gacccactgg
     1861 ctggggggca tggggatcat cgtcctgtcc ctggcggtgc ttccgatgtt gggcgtgggg
     1921 gggacggaac tcctcaaggc cgaggccccg gggcccataa aggaaaaact cactccccgg
     1981 gtgcagcaga ccgccctgat cctgtggggg atctacgtcc tgtttacggt ggtgcagacc
     2041 ggagccctct tggcaggggg gctatcgttc ttcgacgccc tgacccatac cttcggcacc
     2101 gtggccacgg ggggattttc gcccctgaat cagagtgtgg ggcagtacca cagcccctac
     2161 gtggaatggg tgatcaccct tttcatgttc ctgtcggggg cgagttttgc cttgcactac
     2221 cgctttctcc ggggggatgg gagcgcccac tggcgggatc cggagttttg cctctacacc
     2281 ggcatcacgg tgggggccac cgccctggtc acgggggctt tgctggcgtc gggggtttgc
     2341 gatggtcttt ccgatgccct gaggtatgcc gcgtttcagg tggtgagcgt cctcaccacc
     2401 acgggcttcg tcacggcgga cttcgaacag tggccttacg tcacccagtt cgtcctcctg
     2461 ctcctcatgt tcataggggg gtgtgcgggg tccacggggg gaggaatgaa aaacatccgg
     2521 gtgcttctgg tgtggcgcca ggcccgatcg gaactcctgc gcctggtaca cccccggggg
     2581 atcttccccg tgcgagtggg gggagcgccg gtggaacgga gcgtggtgac ctcggtgacg
     2641 gcgtttttta cgctctatgt gatttctttt gccctgtcct ccctggccct gacggggctg
     2701 ggcatggacg tcctgagcgc caccaccggg gtggcggcct gcctgggcaa cgtggggccc
     2761 ggcctgggtt ccgtggggcc catggataac ttttccgggg tgcccctctt gggcaagtgg
     2821 gtgttgtctt tcgacatgct tttggggcgc ctggagatct ataccgtgct tttgatcttt
     2881 ctccgggggg cctggcgagg ataaccatcc ccgggcgcca gggctcacag gctcgcgcgg
     2941 ggacgatgag aacggacgcc ctcggagggc ggatctttta tggagcgccg tcggagtacc
     3001 cgggagagat ccgatgtcag cgaaggccgg ggtatcgcga gatatcctcg gcccttcgcc
     3061 tcggatcggg gcccggacgg cgaaggccac aggaggtgcg ccgaccgtgc atctagccat
     3121 cctctgcagg accctttctt tcgtga
//
LOCUS       DAOOER010000133         3125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_29428_length_3125_cov_5.811075, whole genome shotgun sequence.
ACCESSION   DAOOER010000133 DAOOER010000000
VERSION     DAOOER010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29428_length_3125_cov_5.811075"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            419..967
                     /locus_tag="PL162_09685"
     CDS             419..967
                     /locus_tag="PL162_09685"
                     /inference="COORDINATES: protein motif:HMM:NF037679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQF92329.1"
                     /translation="MDAAEDLIVERGAAGVSLDKVAARAGVSKGGLLHHFPSKNALAQ
                     ALIRRMVDTLHEVRDAERGEGPLDAELIIRTHISWWSRVDSKRRRLYTSLLAATAHDP
                     DLVAPFAWEYEKELQNYEDAGILPGRVSVIMMALHGLWMMELLGITLGDYNQRLFMDE
                     LFRLAKTSSPEGEKERAVDSHA"
     gene            960..2189
                     /locus_tag="PL162_09690"
     CDS             960..2189
                     /locus_tag="PL162_09690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQF92330.1"
                     /translation="MPKRVLMLATLMILALGGYGVVRFVGPANSREVKGASVDQPQAM
                     PVVTALAEVRSFEDSVKVQGTLRAKNFALVSPRSDGMLEEIAVSEGDPVEAGKTLLFR
                     GDTTNLEKRVQMARQDLLAARFAREQQAASVRKTDVELRRAKSDFDRFEALYREQAVS
                     QSVYEDKQAVYDQLQSMRQLVQSGLAMAREAERKAEASLAMAEKDLQDAYMYAPISGV
                     VTKRMAEPGEVGSRGKPILRIDDVGTVEAVAYLPGQFYGDVREGRTSVRLGEEERWER
                     VAPVTYKSSVVDPTLRTFEIRTVLSGDGEHVVPGVLVNMRVVRTERRGVGVPYEALLE
                     RNSGKVIFLTDGQTAREVSVTPGLETGGWVEIVRGEVASGDAVVVQGQSRLSDGSMVT
                     VRNRETKLLSPDSAGER"
     gene            2192..>3125
                     /locus_tag="PL162_09695"
     CDS             2192..>3125
                     /locus_tag="PL162_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQF92331.1"
                     /translation="MSLSQTSLRRPVAMACLLIALTFLGANAYRKMGLELMPKVDAPF
                     ITVVTVWPGATPKDLETDVAKKIEDAVSAVDGLKHVTSVCLENMVQSALEFQLGVDVN
                     VVATDVREKIDLKMDEFPEGVEKPIIQKFNVNSSPIVTLALTGDLPLDQMYDYADHTL
                     RDQLSIISGVANVELLGGAKREVHVLLQRDALGAAGLTSLSVVKALQEGVNSLPSGRL
                     QERGTEYAVRFDAEFQRVEDIGNLQVAGRDGARRFIRDLGTVQMGTEERRQAVFLDGA
                     PGIGIRVVKKADANTVEVVNNVRRAVERLNRNFPG"
BASE COUNT          612 a          862 c         1119 g          532 t
ORIGIN      
        1 gtgccccgcg cccccgcatg cctccgtgcc cccgcgcccc acagaggcca tcccctcgtc
       61 acccctcttt ttcctttccc ggtgcacctc agacgaccca tagaaggagg attgacagat
      121 ccgagacgag ggggtagcat accgtccaga cggtctgttt tttccgagag aggagagagg
      181 ggcatggcaa agaagaaccc atgggagctc cgctccgagg gctccggggg aaagggtagc
      241 gagaaattct cgggccttcc tcggcctaag gagggcggaa agacttcccg gagagcgctt
      301 cgcggcgggg gagaaggcct tgccgtggag cctgacgcgg aagaaaatgg ttcgagcgac
      361 ggcccccccg gaagttaacc ggccggaaag gggagggaaa agcttcagag atgcggtttt
      421 ggatgcggcg gaagacctca tcgtggagcg cggcgcggcg ggggtgagcc tggacaaggt
      481 ggcggcccgg gcgggggtga gtaaaggagg ccttttgcac cactttcctt ccaaaaacgc
      541 cctggcgcag gccctcatcc ggcggatggt ggacacgctc cacgaggtgc gggacgcgga
      601 gcggggggag ggaccccttg acgcggagtt gatcatccgg acccacatct cctggtggag
      661 tcgggtggat tcaaagcgga gacgccttta cacatccttg ctcgccgcca cggcccacga
      721 ccccgacctg gtggctccct ttgcctggga gtacgaaaaa gagctgcaaa actacgaaga
      781 tgcggggatc cttcccggcc gggtgtcggt gatcatgatg gcgctccacg gcctgtggat
      841 gatggagctc ctggggatca ccctggggga ttacaatcag agattgttca tggacgagct
      901 tttccgatta gcgaaaacat cctccccgga gggagaaaaa gaaagggcgg tggattccca
      961 tgcctaaaag ggtcctgatg ctggctacgc tgatgatcct ggcgctggga ggatatggcg
     1021 tggttcgttt tgtggggccc gccaactcgc gagaggtcaa gggggcgtcg gtggaccagc
     1081 cccaggcgat gcctgtcgtg acagctctag ccgaggtgag atccttcgag gattcggtga
     1141 aggtccaggg gaccctccgg gccaagaact tcgccctggt ctctccccgt tcggacggca
     1201 tgctggaaga gatcgccgtg agtgaaggag acccggtgga ggcgggcaag acgctgcttt
     1261 tccgagggga cacgacgaac ttggaaaagc gggtgcagat ggcccgccag gaccttttgg
     1321 ccgcccgttt cgcccgggaa caacaggccg cgtcggtgcg caagactgac gtggagcttc
     1381 gtcgggcgaa aagcgacttt gaccgctttg aagccctgta ccgggaacag gcggtgagcc
     1441 agagcgttta cgaggataag caagccgtat acgatcagct ccaatccatg aggcagctgg
     1501 tgcaaagcgg cctcgccatg gcccgggagg cggagcgcaa ggcggaggcc tctctggcca
     1561 tggcggaaaa ggacctccag gacgcctata tgtacgcccc tatcagcggc gtggtgacta
     1621 agcgcatggc ggagcccgga gaggtgggta gccgaggaaa acccattctg cgcatcgacg
     1681 acgtggggac cgtggaagcg gtggcctacc ttccgggaca gttctatggc gacgtgcggg
     1741 agggaaggac ctccgtgcgg ctgggagaag aggagcgctg ggagcgcgtc gcccctgtga
     1801 cgtataagag ctccgtggtg gaccccaccc tgcggacctt tgagatccgc acggtgcttt
     1861 cgggagatgg ggagcacgtg gtgccggggg tgctagtgaa catgcgggtg gtgcgcacgg
     1921 agcgccgggg agtgggggtt ccctacgagg ccctgctgga gcgcaactcg ggaaaggtga
     1981 tttttctcac cgacggccag accgcccggg aggtgtcggt gacgccgggg ctggaaacgg
     2041 ggggctgggt ggagatcgtc cggggagagg tggcctccgg ggacgcggtg gtggtccagg
     2101 ggcagtcccg gctgagcgac ggatcgatgg tgacggtgcg aaaccgggaa accaaactcc
     2161 tttcccccga ctccgcagga gaacggtagc catgtccctt tcccaaactt cgctccgccg
     2221 tccggtggcc atggcctgtt tgctcatcgc cctgacgttt ctgggggcca acgcctaccg
     2281 aaagatgggg ctggagctca tgcccaaggt ggatgctccc tttatcaccg tggtgaccgt
     2341 ctggcccggg gcgaccccca aagatctgga aaccgacgtg gccaaaaaga tcgaagacgc
     2401 cgtgtccgct gtggacggtc taaaacatgt cacgtcggtg tgcctggaga acatggtcca
     2461 gagcgccctg gagtttcagc ttggcgtgga tgtcaacgtg gtggccacgg acgtgcggga
     2521 gaagatcgac ctgaagatgg acgagtttcc cgaaggggtg gaaaagccca tcattcaaaa
     2581 atttaacgtg aactccagcc ccatcgtgac cctggccctc acgggagatt tgcccctgga
     2641 tcagatgtac gactacgcgg accataccct gcgggaccag ctttcgatca tttccggggt
     2701 ggccaacgtg gagcttttgg ggggcgctaa acgagaagtg catgtgctgc tccagaggga
     2761 tgccctgggg gcggcggggc tcacctccct ttcggtggtg aaggcgctgc aggaaggggt
     2821 gaactccctt ccctcggggc ggctccagga acggggcacg gagtacgccg tgcgctttga
     2881 cgcggagttc cagagggtag aagacatcgg caatctccag gtggcggggc gggacggggc
     2941 gcgccgcttt atccgcgatc tgggcacggt gcagatgggc acggaagagc ggcgccaggc
     3001 ggtatttctg gacggagccc cggggatcgg gatccgggtg gtgaagaagg cggacgccaa
     3061 cacggtggag gtggtaaata acgtccgaag ggccgtggag cggctgaacc ggaacttccc
     3121 cggag
//
LOCUS       DAOOER010000134         3080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_30007_length_3080_cov_10.458182, whole genome shotgun
            sequence.
ACCESSION   DAOOER010000134 DAOOER010000000
VERSION     DAOOER010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30007_length_3080_cov_10.458182"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PL162_09700"
     CDS             <1..498
                     /locus_tag="PL162_09700"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712825.1"
                     /note="catalyzes the interconversion of alpha-D-glucose
                     1-phosphate to alpha-D-glucose 6-phosphate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase, alpha-D-glucose
                     phosphate-specific"
                     /protein_id="HQF92332.1"
                     /translation="SCGFGGEESAGASFLRRDGTVWTTDKDGIIMDLLAAEITAVTGK
                     DPAEHQKDLEARYGAPLYERLDVPATGAQKSALGKLSPALVTARELAGDPITAILTTA
                     PGNGAPIGGLKVTTAEGWFAARPSGTEDIYKIYVESFRGKDHLNRLKEEARALVAAAF
                     VAAGV"
     gene            complement(566..1243)
                     /locus_tag="PL162_09705"
     CDS             complement(566..1243)
                     /locus_tag="PL162_09705"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HQF92333.1"
                     /translation="MGQVQIIVEGMTASGKSTTVELLARRLGLAVMPEEFRDQYDLLR
                     RFHYERRWAFPMQLNFLVTRFAQYLCASEDSVHILDRSIFGDKIYAALYHRLGYFSDG
                     MFQSYLHLYNSLLKHLRMPKLLILLKCPLPEILRRIDVRGREDEIRTGEQYWRDLYTA
                     YDTFLDVVNAEKTIPNLLVLDTATTNLVTNEAHIEAFLKEVRGYLQREDEPDTSSCCP
                     SPCGALA"
     gene            complement(1321..2715)
                     /locus_tag="PL162_09710"
     CDS             complement(1321..2715)
                     /locus_tag="PL162_09710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQF92334.1"
                     /translation="MEHTERMGREPVIKLMLSFALPAMTGILAHALYNIVDRLFVGHW
                     VGPSAIAGIIVAFPFMIAISAVGNFIGVGSATLISLALGNGRRGRAERFLGFAFLMLI
                     VAGSLLTLGGWFFLDPLLWASGASPTILPYAREYTGVIVWGSFFSLTAFTCNSLIRAE
                     GAPYFAMMTLVLGALTNVVLDALFIIVWGMGIQGAALATVISQMISAAWAVSFYFRKQ
                     STLRLRKKRLRPRWSLLRRVASMGIPPFLMEVSFTVLMGVFNHMARRYGGDMALASMG
                     IFFSIDTLLFLPVLGVGEGIQPLVGYNYGAGYYRRTVITAHLGTALCTAFFTMSTLAV
                     QFFPEIMVMAFTRGDDGLLAMTVRAMRLGYMGLPCVGIYMVAVFFLQGLGKATTGMVL
                     SLSRQIFIILPLLVFLPRFWGLDGVWVAFAISDVVGGAMAALFYGAECRRLRRMGEGA
                     ENGEIPSTPQAFIG"
     gene            complement(2851..>3080)
                     /locus_tag="PL162_09715"
     CDS             complement(2851..>3080)
                     /locus_tag="PL162_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005660343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HQF92335.1"
                     /translation="TSMLKRLDVAVFDIIKAKQEGSFQRGVFTYGLKNGGVGLSWSDV
                     AKKNIPADVQQQLKDLEQQIIDGKIVVEDAK"
BASE COUNT          644 a          918 c          996 g          522 t
ORIGIN      
        1 tcctgcggct tcggcggcga agaaagcgcc ggggcgtcct tcctgcggcg ggacggcacg
       61 gtgtggacca cggacaaaga cggcatcatc atggatcttt tggcggcgga gatcaccgcc
      121 gtgacgggga aagaccctgc cgagcaccag aaagacctgg aggcccgcta cggtgcgccc
      181 ctttacgagc gcctcgacgt tcccgccacg ggagctcaga aaagcgccct gggcaagctc
      241 tccccggcgc tggtgacggc ccgggagctg gcgggggatc ccatcaccgc catcctcacg
      301 acggctccgg gcaacggggc ccccatcggg ggactcaagg tcaccacggc ggaggggtgg
      361 tttgccgccc gcccttcggg caccgaggat atctacaaga tctatgtcga gagctttcgg
      421 gggaaagacc acctgaaccg gttgaaggaa gaggcccggg ccctggtggc ggcggctttt
      481 gtcgctgcgg gagtctaaag gcgcgaaaag aagctcccgg aagcgttaac gcttccggga
      541 gcttcttttc gcttatgacc cggccctacg ccaaggcgcc gcagggtgaa gggcagcagg
      601 acgacgtgtc gggttcgtcc tcgcgctgga ggtagccccg aacctccttc aagaaggctt
      661 caatgtgggc ctcgttggtc accagattgg tggtggcggt gtcgagcacc agcagattcg
      721 gaatggtctt ttccgcgttc accacatcca ggaacgtgtc gtaggcggtg tagagatcgc
      781 gccagtactg ctctcccgtc cggatttcgt cctcgcgccc ccggacgtcg atgcgccgca
      841 ggatctccgg cagggggcac ttgagcaaaa tcaggagctt gggcatccgg aggtgtttca
      901 gcagcgagtt gtacagatgg agatagctct ggaacatgcc gtcggagaag taccccaacc
      961 ggtggtacag ggcggcgtag atcttgtccc cgaagatgct gcggtccagg atgtggacgg
     1021 agtcctccga ggcgcagaga tactgggcga accgggtgac cagaaaattg agctgcatgg
     1081 gaaaggccca gcggcgctcg tagtgaaagc gccgtaacaa gtcgtactgg tcccggaact
     1141 cctcgggcat gacggcgagc cccagccgac gagccagcag ctccacggtg gtggatttgc
     1201 cgctggcggt catcccttcc acaatgatct gtacctgccc cacgaccatc tctcctgtct
     1261 ttggtgaagc ttatgccctg cgggccaggg cgccccgttc ccgggcattg tagcacgttt
     1321 ttacccgata aaggcctggg gagtggaggg gatctccccg ttctcggccc cctctcccat
     1381 ccgacggagc cgtcgacact ccgccccgta gaagagcgcg gccatggcgc ccccgacgac
     1441 gtcggaaatg gcgaaagcga cccacacccc atcgagcccc cagaaacggg gcaggaagac
     1501 cagcaggggc aatattatga aaatttgccg agaaagactc aggaccatgc cggtggtggc
     1561 ctttcccagt ccttgaagga aaaagaccgc caccatgtag atccccacgc aggggagtcc
     1621 catgtaaccc aggcgcatgg ctcgcaccgt catggccagc aggccatcgt cccctcgggt
     1681 aaaggccatg accatgatct ccgggaaaaa ttgtaccgcc agggtactca tggtgaaaaa
     1741 cgccgtgcat agggcggtgc ccaggtgggc ggtgatgacg gtgcgtcggt agtatcctgc
     1801 gccgtagtta taccccacca agggttggat gccttctccc actcctaaaa ccgggaggaa
     1861 gagcagggtg tcgatgctga aaaagattcc catggaggcc agggccatgt ctccgccgta
     1921 ccgccgggcc atgtggttga aaactcccat gaggaccgtg aaagagacct ccatgaggaa
     1981 aggcggaatg cccatggagg ccacccgtcg cagcaggctc caccgggggc gaagccgctt
     2041 cttccggagg cgcaaggtgc tctgctttcg gaagtagaag gatacggccc aggcggcgga
     2101 gatcatctga gatatcacgg tggccagggc ggcgccctgg atccccatgc cccagacgat
     2161 gatgaagagg gcgtccagca ccacgttggt cagggccccc aacaccaggg tcatcatggc
     2221 gaagtaggga gctccctcgg cgcgaatgag gctattgcaa gtgaacgccg tgagggagaa
     2281 gaaggatccc cagacgatga cgccggtgta ctcccgggcg taggggagga tggtggggct
     2341 ggctccgctc gcccagagca agggatccag aaaaaaccac ccccccaggg tgagaaggga
     2401 gcctgcgacg atcagcatga ggaaggcaaa cccgaggaat cgttcggccc tcccccggcg
     2461 accgttcccc agggccaggg agatgagggt ggccgacccc acgccgatga agtttcccac
     2521 ggcggaaatg gctatcatga agggaaaggc cacgatgatc cccgcgatgg ccgaaggtcc
     2581 cacccagtgc cccacgaaga ggcgatccac gatgttgtag agggcatggg ccaggatacc
     2641 ggtcatggcc gggagggcga aggagagcat gagctttatg accggctcgc gccccatgcg
     2701 ttcggtatgt tccatggtag gtacgattcc tccttgaaga aaaaaagcgg gtcctccgct
     2761 tcgagcgaaa ggacccgacg atggggcagt tccccggggt aagagtcgcc gaaggggaag
     2821 gaggaaccgg atcagaaagg gtccggtcga ctacttcgca tcctccacga cgatcttgcc
     2881 gtcgatgatc tgctgttcga gatccttcaa ctgttgctgc acgtcggcgg gaatgttctt
     2941 ttttgccaca tcgctccagg agagccccac gccgccgttt ttgaggccgt aggtaaagac
     3001 ccctcgctgg aaagagccct cctgcttggc cttgatgatg tcaaataccg ccacgtccag
     3061 ccgcttcagc atgctggtca
//
LOCUS       DAOOER010000135         3044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_30506_length_3044_cov_5.948478, whole genome shotgun sequence.
ACCESSION   DAOOER010000135 DAOOER010000000
VERSION     DAOOER010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30506_length_3044_cov_5.948478"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1082)
                     /gene="dctP"
                     /locus_tag="PL162_09720"
     CDS             complement(72..1082)
                     /gene="dctP"
                     /locus_tag="PL162_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HQF92336.1"
                     /translation="MFKKRLLALSVCVAVFGIFLASSAFAAPEMTLKFAGQSPADHTA
                     TKFMEDIAKEVAEKTGGRIEIKVYPANQLGDYTLVYEELIRGTIDMAGISIPSQFDPR
                     MELVYINGFVRGYEDAKRIFTPSGWLFKKMDELNQALGVKLTGFYVEGFIGTGTTKPA
                     KDPLDPKVEKGLLIRVPNMDVYMLAAQAMGYKTITIPYADVYQSMQTGVCDGVNGYPV
                     AAAYSILGDVLKYWYNTNYSIECLNYMVSGKTWEKLSDADKQIIQDAFTNATLKSIDN
                     AKSEDEKYMDLMRKKGIEVFTYTDEELKPISEACAATWPKLEEKMTKELIDEFRKELA
                     PK"
     gene            complement(1611..2696)
                     /locus_tag="PL162_09725"
     CDS             complement(1611..2696)
                     /locus_tag="PL162_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008710141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HQF92337.1"
                     /translation="MILAGLGLIWLGIKKEYEPALLIPIGFGAILVNIPFSSALTQLV
                     DGKIQEGALSTLFHFGITTEMFPLLIFLAVGAMIDFGPLLQKPVMFIFGLTAQIGIFV
                     AMMLALYFGFDLKEAASIGIIGAADGPTSIFVAAKFAPHLLGPISVAAYTYMSLVPII
                     QPPVMRLLTTEAERRIRPVPRPEHSVSRDVRIMFPIAVTILASMIAPASASLIGFLML
                     GNLIRECGVLERLSKGAQNELANIVTLLLGISISATMTGEQFLRADTLIVIAMGLVAF
                     IFDTAGGVITAKVMNLFLKEKINPFIGAAGISAFPMASRVVQKMGQEYDRGNHLLMHA
                     VGANVAGQIGSVLAGGLLLSFIGRMAG"
BASE COUNT          619 a          831 c          999 g          595 t
ORIGIN      
        1 cgacctcaca tccgttccgg aacggttttc ccgtcctggg caagggctgt ccggacagag
       61 gggtgatggg cttatttcgg cgccagctcc ttgcggaact cgtcgatgag ctctttggtc
      121 attttctctt cgagcttggg ccaagtggcg gcgcaagcct cggagatggg cttcagctct
      181 tcgtcggtgt aggtgaagac ctcgatgccc tttttccgca tgagatccat gtacttctca
      241 tcttcggact tggcgttgtc gatgctcttg agggtggcgt tggtgaaggc gtcctggatg
      301 atctgcttgt ccgcatcgga gagcttttcc caggtcttgc cgctcaccat gtagttcagg
      361 cactcgatgg agtagttcgt gttgtaccag tacttcagca cgtcccccag gatggagtag
      421 gcggcggcca cggggtagcc gttcacgccg tcgcacacgc cggtctgcat ggactgatag
      481 acgtcggcgt aggggatggt gatggtcttg tagcccatgg cctgggcggc gagcatgtag
      541 acgtccatgt tgggcacccg gatgagcagg cccttttcca ccttaggatc gaggggatcc
      601 ttggcgggtt tggtggtgcc ggtgccgatg aagccctcca cgtagaatcc cgtgagcttc
      661 acgcccaggg cctggttgag ctcgtccatc ttcttgaaga gccatcccga gggggtgaaa
      721 atccgcttgg catcttcgta gccgcgcacg aagccattga tgtagacgag ttccatgcgg
      781 gggtcaaact ggctgggaat ggagatcccc gccatgtcga tggtgccgcg gatgagctct
      841 tcgtagacca gagtgtagtc ccccagctgg ttcgcggggt agactttgat ctcgatgcgg
      901 ccgccggtct tttccgccac ttccttggcg atgtcttcca tgaactttgt tgccgtgtgg
      961 tccgcagggg actgtccggc gaacttcaga gtcatctccg gagcggcaaa agcggaggaa
     1021 gccagaaaga tcccaaaaac cgcgacgcaa accgacaacg cgagcaaacg cttcttgaac
     1081 atggtcaccg ataacatctc cttcccgaag gtgcgaaaaa atctcccatt gccccgtttc
     1141 cgtgatcgtg gctccagacg acgctccctt tccccgtggt ctgtcgtgcc cgctacctaa
     1201 gcctcacccc cttaacgacg tgcggtttgg cgtctgtgcg cctggcgcgg aggaaacccc
     1261 ctggggacct tcctcggatg cccacccctt tgtaccattg atccgccctt caaacaaggg
     1321 ccgacttgct cccctctccc ggacgaggta gaccaggagg cttttccaga aattgacacc
     1381 ggaggagtgc cccccgggga ggagcgtaga aacatcagac atatcctaca cgccattata
     1441 agcacgggtt tcctctttga ctaggggatg ccccgcagga caggggcttc caggaggaga
     1501 aatattgtgt ggagcatacg aaaaacggcg cccccctttg aaggcagggg gcgccgaagg
     1561 tttttccgta aaggggaatc tttccccaaa ggcctcgtta ggggcgaggc ttaccccgcc
     1621 atgcgaccga tgaaagagag caagagcccc ccggcgagga ccgaaccgat ctgccccgcc
     1681 acgttggcgc ccacggcgtg catgagaagg tgatttcccc ggtcgtactc ctgccccatc
     1741 ttctgcacca cccgggacgc catggggaag gcggaaatgc ccgcagcgcc gatgaagggg
     1801 ttgatctttt ccttcagaaa gaggttcatg accttggcgg tgatcacgcc cccggcggta
     1861 tcaaagataa aagccacaag gcccatggcg atgacgatga gggtgtcggc gcgcaggaac
     1921 tgctctcccg tcatggtggc gctgatggaa atgcccagaa gcagggtgac gatgttggcc
     1981 agttcgttct gggctccctt ggagaggcgc tccaacacgc cgcactcccg gatgaggttc
     2041 cccagcatga gaaagccgat gagggaggcg gaagcggggg ctatcatgct ggccagaatg
     2101 gtgacggcga tggggaacat gatgcggacg tcgcgggaca cggagtgttc cggccggggc
     2161 acggggcgaa tccgccgctc cgcttccgtg gtgaggaggc gcatcaccgg gggctggatg
     2221 atgggcacca gggacatgta ggtgtacgcc gccaccgaga tggggcctaa aaggtggggg
     2281 gcgaacttgg ccgccacaaa gatggaggtg gggccatcgg cggcgccgat gatgccgatg
     2341 gaggccgcct ccttgaggtc gaagccgaaa tagagggcca acatcatggc cacgaagatg
     2401 ccgatctggg cggtgaggcc gaaaataaac atgacgggtt tctggagcag gggaccgaag
     2461 tcgatcatgg cccccaccgc caggaagatg agcaggggaa acatttcggt ggtgatgccg
     2521 aagtggaaga gggtggagag ggccccttcc tgaatcttgc cgtccacgag ctgggtgagg
     2581 gccgaggaaa agggaatgtt caccaaaatg gcgccgaacc cgatgggaat gagcagggcg
     2641 ggttcgtact ccttcttgat gccgagccag atgaggccga ggcccgcgag gatcataatc
     2701 acctgtttgt acgtgatggc ggcgagaacg agcacgaaag atcatccttt ctcaaggccc
     2761 tagggggcga caaccagaat ttccgggagg aagaaacgac cctccggagg gaagcagagg
     2821 cccattctat cacgagaggg ggtgaatgcg aagaatgccc ccggctgtgt tagaatgcgg
     2881 ggcaaagatg tcacttttaa gggaggagcg atgccgatgt ccgttttcag cggtcccgag
     2941 ggacagcgtt tagagggaaa cgatctgtcc gcaggggaga ttctccggcg gtggcagtgc
     3001 gataaggggg cggtggcggc ccgggtgaac ggccggatgg tgga
//
LOCUS       DAOOER010000136         3043 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_30521_length_3043_cov_6.355422, whole genome shotgun sequence.
ACCESSION   DAOOER010000136 DAOOER010000000
VERSION     DAOOER010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30521_length_3043_cov_6.355422"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PL162_09730"
     CDS             <1..281
                     /locus_tag="PL162_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HQF92338.1"
                     /translation="ISSNAGLQGDVVVDRVRTLPKGHGLNAATGEYEDMVAAGIIDPM
                     KVTRSAVQNAGSIAAMVLTTESLVADRPEKKDAPKMPGGMGGMGDMDY"
     gene            525..2063
                     /gene="guaA"
                     /locus_tag="PL162_09735"
     CDS             525..2063
                     /gene="guaA"
                     /locus_tag="PL162_09735"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HQF92339.1"
                     /translation="MDHKQMVVVLDFGSQYTQLIARRIREQRVYSEILPWDAPPEEVL
                     RRSPRGIVLSGGPASSLEPGAPVVAPEILASSIPILGICYGMQALTRSLGGTVRQGVR
                     AEYGRTRVKLLGSHALFEGVDRDLTVWMSHWDQVETIPPGTVLLAQSEGGVPAAFATP
                     EGLIGALQFHPEVVHTASGALILANFLFRICRCQPDWDLGEWIQHSMESVRQAVKGDR
                     VVCGLSGGVDSSVAALLVQKALGGRLTCIFVNNGLLRLGEAEEILQAYQGLGLDVRYV
                     DASDRFLDALAGVTEPERKRKIIGEVFVRVFEEAAQGTGGAQWLLQGTLYPDVIESGQ
                     KGRGAAVIKTHHNVGGLPEDMALGLLEPLRDLFKDEVRAIGRMLQLPERILGRHPFPG
                     PGLGVRCLGELSRARLDILRQADALFLRALREANLYDQVWQAFAALLPVRSVGVVGDV
                     RTYGETVVLRAVEAQDGMTADWARLPYDLLDRVARSICNEIPGVSRVVYDITGKPPAT
                     IEWE"
     gene            2449..2970
                     /locus_tag="PL162_09740"
     CDS             2449..2970
                     /locus_tag="PL162_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HQF92340.1"
                     /translation="MSFERVDTDEAISIEDCAAINKRLDVKIVKTLFLCNRQQTSFYL
                     FVTKGDKPFSSKAFSQALGVSRVSFAPVERMEAMLGTKVGAATVFSALWDKGNDVQMV
                     FDKDVLAEEWYGCSDGTTTGYMKIRTEAIVRDFLPFANHRILVIGGQFPPGVVSMDES
                     KEMRGEGNSSGGP"
BASE COUNT          599 a          794 c          997 g          653 t
ORIGIN      
        1 tcatttcctc caatgcggga ctccagggcg acgtggtggt ggatcgcgtg cgcacccttc
       61 ctaaggggca tggcctgaat gccgccaccg gcgagtacga ggacatggtg gcggcgggga
      121 ttatcgaccc catgaaggtg actcgcagcg ccgtgcagaa cgcgggttct attgccgcca
      181 tggtgttgac tacggagagc ctcgtggcgg atcgccccga gaaaaaggat gcccccaaga
      241 tgcccggcgg catgggcggc atgggagaca tggattacta ggataaaaga ccacctcggc
      301 ccccgctgaa aggcgggggc cttttgcgtt gtcttgattt gtgatgcggt aaaaacggtc
      361 tcctatctgg tatcatggcc cagacgccga tcggccctgg gcgggggatt cgggacggaa
      421 ggcgccgaag ggtgtgacaa tggaagccga agacatccag cccgaggccg acggacctcg
      481 ggggagcttt tttgaaagag gtgagtgccc tttcgggcct tttgatggat cacaaacaga
      541 tggtggtagt cctagacttc ggatcgcagt acactcagct tatcgcgaga aggattagag
      601 agcaaagggt ttatagcgaa atcctcccct gggatgcccc tcctgaagag gtcctgagac
      661 gatctccccg ggggatcgtc ctttccggag ggcccgccag ctcgctggaa ccgggggctc
      721 ccgttgtggc tccagagatt ctggcctcat ccatccctat cctgggcata tgctatggca
      781 tgcaagccct cacgcgatct ttggggggta cagtgcgcca gggagttagg gcggagtatg
      841 gccgtacccg ggtgaagctt ttaggttctc atgccctatt tgagggggtg gatcgggatc
      901 tgacggtgtg gatgagtcat tgggaccagg tggaaaccat tcccccaggg actgtcttgt
      961 tagcccaaag cgaagggggg gtcccggcgg cctttgccac tccggagggg ctcatcgggg
     1021 cgctacaatt ccaccccgag gtagttcaca ccgcttcggg ggcattgatc ctggcgaact
     1081 tcctgtttag gatttgtcgc tgccagcccg attgggacct gggggaatgg atacagcaca
     1141 gcatggaatc cgtgcggcag gcggtaaaag gagatcgggt cgtctgtggc ctctcggggg
     1201 gagtggactc ctccgtggcg gccctgttgg ttcaaaaagc cctggggggg cgtcttactt
     1261 gcatctttgt gaataacggc ctcctgcgcc tgggagaagc ggaggaaatc cttcaggcct
     1321 accaggggct tggtttggac gtacggtatg tagacgcctc ggatcgcttt ctcgacgccc
     1381 tggcgggggt gacggagccc gagaggaaga gaaaaatcat tggcgaggtc tttgttcgcg
     1441 tcttcgaaga ggcggctcag ggcacggggg gggcccagtg gcttctgcag gggaccctct
     1501 atcccgacgt catagagagc ggtcaaaaag gacgtggcgc cgcagtgatc aagactcatc
     1561 acaacgtggg ggggttgccg gaagatatgg ctctggggct tctggagcca ctgcgagacc
     1621 tgttcaaaga cgaagtgagg gccatcgggc gtatgctcca gctcccggaa aggattttgg
     1681 ggcgccaccc ctttcccgga ccgggattag gggtgcgatg cctgggagag ctctcccggg
     1741 cccggttgga catcctgcgt caggcggatg cccttttcct gcgggctctg cgagaggcca
     1801 acctttacga ccaggtgtgg caggcctttg cggccctttt gcccgtgcgt agcgtgggcg
     1861 tggtgggaga tgtgagaacc tacggagaga ccgtggtctt gcgggctgtg gaggctcagg
     1921 atggcatgac ggcggattgg gctcgtctac cctacgacct gttggatcgc gtggcacgct
     1981 ccatctgcaa cgagatcccc ggggtgagcc gcgtggtgta tgacatcaca gggaaacccc
     2041 ccgccaccat cgaatgggaa taacccgtgg ggaccgttcg acacggggga tccatccttt
     2101 ggctgccccc cccatggcca gcgtcccagg tctctcccgg gcctggagga ggcctttcgc
     2161 cgttcgtttc ggggtggagt ctcggcttat cccgaagaag gcgccctttc ccgggccctc
     2221 tctagcgagg cgatgacggc cctcggcgat ctttcggggc cggaggagga atggggggga
     2281 ctttgtccgg ggcgcgatgg ggtagcgttt ttccgtgtag cgtcatgatg ataaggttga
     2341 taaaaagagg atccgatgat ttatgtaagt aatgtaaaaa cttcacctcc ggagatctat
     2401 aagacgccac tccaaagtct agtctatcag accttggagt tgttggattt gagcttcgaa
     2461 agagtggata cggacgaggc catctccatt gaagattgcg cagctattaa taaaaggctg
     2521 gatgtgaaaa tagttaagac tctgttcctg tgcaatcgtc agcagacgtc gttttatctt
     2581 tttgttacta aaggggataa accctttagc tccaaggcgt tcagccaggc cctaggggtg
     2641 tctcgggttt cctttgcccc cgtggaaaga atggaggcca tgctgggcac caaggtcggg
     2701 gcggctacgg tgtttagcgc cctgtgggat aagggaaacg acgtgcagat ggtcttcgac
     2761 aaagacgtcc ttgcggaaga gtggtatggg tgcagcgacg gcaccaccac gggttacatg
     2821 aaaatccgca cagaggcgat agttcgagac tttcttccct tcgcaaacca taggatctta
     2881 gtcataggag ggcaatttcc cccaggggtg gtctccatgg atgaatctaa ggaaatgcgc
     2941 ggagaaggta actcttccgg gggcccctag ggggcccctt ttttcaccca tactccatcc
     3001 ataatattcc tccatcggcc ttggatcccc tccgatcgtg cca
//
LOCUS       DAOOER010000137         3036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_30636_length_3036_cov_6.886615, whole genome shotgun sequence.
ACCESSION   DAOOER010000137 DAOOER010000000
VERSION     DAOOER010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30636_length_3036_cov_6.886615"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="PL162_09745"
     CDS             complement(<1..754)
                     /locus_tag="PL162_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQF92341.1"
                     /translation="MTTTVGQKGVGEGELEGRAQSNRSKGVEIEGVTKIFRDPQSGRD
                     VVGVDDISTTIAPGELVTLLGPSGCGKTTTLRMIAGFELPTKGKILLGGEDITTLPPN
                     HRDTAMVFQSYGLFPHMNVFDNVAYGLKLRKVGREALREKVGRFLRLVGLEELAERPP
                     SRLSGGQQQRVALARALIVEPSVLLLDEPLSNLDALLRESMRVEIRRIQRSLGITAVY
                     VTHDRTEAMSLSDRIVVMHKGRIVQTGTPREIY"
     gene            complement(849..3035)
                     /locus_tag="PL162_09750"
     CDS             complement(849..3035)
                     /locus_tag="PL162_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015557193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HQF92342.1"
                     /translation="MRSTAVALVLFALGVYGVLHSLGGAFDATLERERRATALAALAS
                     PPRDAEGLSSWMGRLEESAPPFDLLYIVGTPAPGEEKGLFATAPSLEAFYRSQGSSKN
                     FIKGMESASYEETYAPGKVFDVGGRRCFLLFAPAVEPRTREILGVGVFAFDAAPREEF
                     FRLIYILGGGAFVLFAVILLVGRFSRDPLVGYAIAFLFAGTGVFVAYPLFEALRLSLT
                     SQGEFSLEIWRSVFQSSQYLSALWGSIRLGAWTATLSTVLGFLFAFVLGRTNVRGKKF
                     FTAMATLPVISPPFSLTLSVILLFGNNGLVTKNLFGSPAISIYGLGGLALVQTMGMFP
                     IAFLTLAGILEAIDSTLEDAALDLRADRWTTFRTVTLPLCMPGILSAWLLVFTNSLAD
                     FANPLILSGSYRVLSVEAYMEVTGMNRLGHGAALSLLLLLPTLSAFFAQRFWVGRKSF
                     VTVTGKPSGRLTELTSPGVKKVLVGAMVLVTLFLLALYGTIVAGCFVKNWGIDYTFSL
                     ANIREAFSRGKDALRDTVILASAATPIAGILAMVAALLLVRKAFAGKRLLEALLLTPF
                     ALPGTLMGISYILAFNKPPLVLVGTAAIIVINYVVRELPVGVEGGIAALRQIDPSIEE
                     AAADLGADAATVFRDVVLPLVRPAFISSLSYTFVRSMTAVSAVIFLISARWYHLTVLI
                     YNFSENLRFGLASVLATALILIVLATFGLLRLLVPKNVHLAKTLGR"
BASE COUNT          662 a          953 c          951 g          470 t
ORIGIN      
        1 ggtagatctc ccggggcgtg cccgtctgga cgatgcgccc cttgtgcatc accacgatgc
       61 ggtccgacaa gctcatggcc tcggtgcgat cgtgggtgac atacacggcg gtgattccca
      121 gagagcgctg gatgcgccgg atctccaccc gcatggactc ccgcaacagg gcgtccagat
      181 tggaaagggg ttcgtccagc agtagcaccg agggctccac aatgagcgcc cgggccagag
      241 ccacccgctg ctgctgtccc cccgacagac gggagggggg acgctccgcc agctcctcca
      301 accccaccag ccgcagaaaa cgccccacct tttccctaag ggcctcccgc cccaccttcc
      361 gcagcttgag gccgtaggcc acgttatcaa aaacgttcat gtgaggaaaa aggccgtagc
      421 tctgaaagac catggcggtg tcgcggtggt ttgggggcaa cgtggtgatg tcctctcctc
      481 ccaggaggat tttcccctta gtgggcagtt caaaccccgc aatcatgcgg agcgtggtgg
      541 tttttccgca ccctgaagga cccagcaggg tcaccagttc ccccggagcg atggtggtgg
      601 aaatatcgtc cacccccacc acatcccggc cactctgggg atcgcggaaa attttggtga
      661 ccccctcgat ctccaccccc ttggaacggt tgctttgagc ccttccttcg agctctcctt
      721 cgccgacacc tttttgcccc accgtcgtag tcatgctcct ccacctttcg gccaggccat
      781 gatttttatc ttccgccgat cctctgcccc gggggttggg gcgcctcccg atatttcttg
      841 ccggagggtc aacgccccaa ggttttcgcc agatggacgt tcttgggcac caaaagccgc
      901 agaaggccaa aggtggccag cacgatgaga atgagcgccg tggccagcac gctggccagg
      961 ccgaaccgaa ggttttccga gaagttgtag atcaacaccg tgagatggta ccaccgggcg
     1021 gagatgagga agatcaccgc actcaccgcc gtcatggagc gaacaaaggt gtaggacaga
     1081 ctggaaataa aagcgggacg caccagagga aggaccacgt cccggaagac cgtggcggca
     1141 tcggccccca aatccgccgc agcttcctcg atggaggggt cgatctggcg gagggcggcg
     1201 atgcccccct ccactcctac gggaagctcc cgcaccacgt agttgatcac gatgatggcg
     1261 gcggtgccca ccaacaccag aggaggctta ttaaaggcca agatgtagct tatccccatg
     1321 agggttcccg gcagggcgaa gggggtgagg agcaacgcct ccagcaagcg cttccccgcg
     1381 aaggcctttc gcaccagcag gagggccgcc accatggcca ggatccccgc gatgggcgtg
     1441 gcggccgagg cgaggatcac cgtgtctctc agggcgtcct taccccggga aaaggcctct
     1501 cggatgtttg ccaggctaaa ggtgtagtcg atgccccaat ttttaacgaa acatcccgcc
     1561 acgatggtgc cgtagagggc caaaaggaag agggtcacga ggaccatggc ccccaccagg
     1621 acttttttca ccccggggga agtgagctcc gtgagcctcc ccgaaggctt acccgtcacc
     1681 gtgacgaagg acttgcgccc cacccagaag cgctgggcga agaaggcgct caaggtgggt
     1741 aaaagcagca ggagggagag ggccgcccca tgtcccaagc ggttcatgcc cgtcacctcc
     1801 atgtaggcct ccacggagag cacccgatag ctccccgaga ggatgagggg attggcgaag
     1861 tccgccaggg agttggtgaa aaccagcagc caggccgaga ggatgccggg catgcagagg
     1921 gggagggtga cggtgcggaa ggtggtccat cggtcggccc ggagatctaa cgccgcatcc
     1981 tccagggtgg agtctatggc ctccaggatg cccgccagag tcagaaaggc gatgggaaac
     2041 atccccatgg tctgcaccag ggcaagcccc cccagaccgt agatggatat ggcaggggat
     2101 ccgaagaggt ttttcgtcac caggccgttg ttgccgaaga gcagaatcac cgacagggtg
     2161 agggagaagg gaggagagat caccggcagg gtggccatgg cggtgaaaaa cttcttgccc
     2221 cgcacgttgg tgcgccccag cacaaaggca aagagaaagc ccaacacggt ggatagggtg
     2281 gccgtccagg cccccaggcg gatgctcccc cagagggccg aaaggtactg agagctctgg
     2341 aacaccgatc gccagatctc cagggaaaac tccccctggg aggtcaggga aaggcgcagg
     2401 gcctcgaaga gggggtaagc cacaaagacc cccgtcccgg cgaagagaaa ggcgatggcg
     2461 tatcccacca ggggatcccg gctgaagcgc cccaccaaca aaatgacggc gaagaggaca
     2521 aaggcccctc ccccgaggat gtagatcagg cgaaaaaact cctcccgggg agcggcatcg
     2581 aaggcgaaca cccccacccc caggatttcc ctggtgcggg gctccaccgc aggggcaaag
     2641 agcagaaagc agcgccgtcc ccccacatcg aagacctttc ctggggcata ggtctcctcg
     2701 taggaggcgc tctccatccc ttttatgaaa tttttggaag atccctggga tcgatagaag
     2761 gcctcgagag agggagcggt ggcaaaaagc cccttctcct cccccggggc aggggtgccg
     2821 acgatgtaaa gcaggtcaaa gggaggggcg ctttcctcca ggcgccccat ccaggacgaa
     2881 agcccctccg catcccgggg aggggaggcc agggccgcca gggccgtggc ccggcgctcc
     2941 cgttccaagg tggcgtcaaa ggctcccccc agggagtgca acaccccgta aaccccgagg
     3001 gcgaaaagga ccaacgccac cgctgtgctc ctcatc
//
LOCUS       DAOOER010000138         2982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_31425_length_2982_cov_4.944653, whole genome shotgun sequence.
ACCESSION   DAOOER010000138 DAOOER010000000
VERSION     DAOOER010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31425_length_2982_cov_4.944653"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="PL162_09755"
     CDS             <1..953
                     /locus_tag="PL162_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HQF92343.1"
                     /translation="QAALVPLEIEEPTVSMFFLVNTGPLAGQEGKAVTLRQIRERLER
                     ELKSNVSLRMEDLGRADGLKVSGRGELHLAILIEEMRREGLEFCVSRPEVITKRDAEG
                     NLTEPMEQLIVDVPEEYQGVVIEKLSRRKGELAGVFPTGPSTVRLEFTIPTRGLIGYR
                     SDFLTDTRGLGILSARMIGYDLWRGEVSGRIAGSIVSMDTGDATAYQLDNLQSRGTLF
                     IGPLERVYEGMVVGENSRPSDIPCNPTKKKHLSNMRSATKDSTVVLKVPRRLTLEAAL
                     EWIADDELVEITPQSIRVRKSILNAEARKKAERRKDLEGA"
     gene            1003..1575
                     /locus_tag="PL162_09760"
     CDS             1003..1575
                     /locus_tag="PL162_09760"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HQF92344.1"
                     /translation="MPHILGQRILLREYRLSDLSEMRKWVNDPRVTRGLSDIFLFPHS
                     QEATEEFLRDVLEGRSDTREFVVASRPSEAYLGQIGLKVLDWKNRFAEISLVFPREHQ
                     GQGYGTEAIGLLQRFAFEEMGLNRLELFVHEDNAPALRCYQKCGFREEGRFRQRFFRQ
                     GRFWDMILMALLAEEYRSFPAPAEEREDFV"
     gene            1568..2137
                     /locus_tag="PL162_09765"
     CDS             1568..2137
                     /locus_tag="PL162_09765"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HQF92345.1"
                     /translation="MSDITGDRVRLRAYRESDIPALREWVNDPQVTRFLEGLSLFPHT
                     LHDTESFVRAQLAPRPLHPESLELVVADRHTDVYLGQVGLLAIDWPNRATALGIVFPR
                     KNQGKGYGREALGLLKGLVFDDLGLHRLELEVHEDNAPALRCYQKCGFREEGRRRERR
                     FSGGRFWDVVIMALLAPEYRASKISRGKE"
     gene            2243..>2982
                     /locus_tag="PL162_09770"
     CDS             2243..>2982
                     /locus_tag="PL162_09770"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HQF92346.1"
                     /translation="MPHPSAPRSRTKGAEAPPAALVLAAGRSSRMGCGKMLLPLGDST
                     VLAQAVTRLREGGAHPVMVVTGHHREAVEAEARRLGCPTVANPDPEGDMFSSLRVGVA
                     ALPPKTPAVLVLPGDTPLVRPSTGRALIAALRGADAAIPTFRQQGGHPVLLGRAALAS
                     LALRPAPEGLRSVLSSPELRVCRVPVPDSAILRDADTPEDFAALTAHAAREKIPTEEE
                     VEALLELHRTPQEVRRHAQATARAARALG"
BASE COUNT          494 a         1028 c          975 g          485 t
ORIGIN      
        1 cccaggcggc cctggttccc ctggagatcg aggagcccac ggtgtccatg ttctttctgg
       61 tgaacacggg ccccctggcg gggcaggagg gcaaggccgt caccctgcgc caaattcgag
      121 agcggctgga gagggagctt aaaagcaacg tttcgctgcg catggaggac ctgggcaggg
      181 ccgacgggct gaaggtttcg gggcggggag agctccatct ggccattctc atcgaagaaa
      241 tgcgtcgaga ggggctggag ttctgcgtct cccgcccgga ggtcatcacg aaacgcgacg
      301 ccgagggaaa cttgacggaa cctatggagc aactcatcgt ggacgtccct gaggagtacc
      361 agggagtggt catagaaaag ctctcccggc gcaaggggga gttggcgggg gtcttcccca
      421 cggggcccag caccgtgcgc ctggagttca ccatccccac ccgggggctc atcggctacc
      481 gcagcgactt cctcacggac acccgggggc tggggatcct ctcggcccgc atgatcggct
      541 acgatctctg gcgaggggag gtctcggggc gcatcgcggg ctccatcgtg agcatggaca
      601 cgggggacgc cacggcctac cagctggaca acctccagtc ccgggggacc ctcttcatcg
      661 gccccctgga aagggtctac gaggggatgg tggtggggga aaactcccgt cccagcgaca
      721 tcccctgcaa ccccacgaag aaaaaacacc tctccaacat gcgttccgcc accaaagaca
      781 gcaccgtggt gctcaaggtg ccccggcgcc tcaccctgga agcggcgctg gagtggatcg
      841 ccgacgacga gctggtggag atcacccccc agtccattcg ggtgcgcaag agtatcctga
      901 acgccgaggc ccggaaaaaa gcggagcggc gcaaggacct cgagggggcg tagcgccgtc
      961 gccccgaggg gaagcctaga aagctaaagg ggaggccttt tcatgccgca cattctggga
     1021 caacggatcc tccttcggga atatcgcctc tccgacctgt cggagatgcg aaagtgggtg
     1081 aatgaccccc gggttacccg ggggctgtcg gacatctttc tctttcccca ctcccaggaa
     1141 gccacggaag agtttctccg cgacgtgttg gaggggcgtt ccgatacccg ggagttcgtg
     1201 gtggcgtctc gcccctcgga ggcctatctc gggcagatcg gtctgaaagt cctcgactgg
     1261 aaaaaccgct tcgccgagat ctccttggta ttcccccggg aacaccaggg gcaggggtac
     1321 ggcaccgaag cgataggcct gctccagcgt ttcgcctttg aggagatggg gctaaaccgt
     1381 ctggagctct ttgtccacga ggacaacgcc cccgccctcc ggtgctacca aaagtgcggc
     1441 ttccgggagg aggggcgctt taggcagcgc tttttccgtc aggggcgctt ctgggacatg
     1501 atcctcatgg ccctcctggc cgaagagtac cgatccttcc cggccccggc cgaggagcga
     1561 gaagactttg tctgatatca ccggggatcg ggtgcgcctc cgggcctacc gggagtcgga
     1621 catccccgcc ctgcgggagt gggtcaacga cccccaggtg acgcgctttc tggaggggct
     1681 ctcccttttt ccccacaccc tgcacgacac cgaaagcttt gtccgggccc agctcgctcc
     1741 ccgccccctc catccggaat ctctggagct ggtggtggcg gatcgccaca ccgacgtcta
     1801 tctggggcag gtggggcttc ttgccatcga ctggcccaac cgggccacgg ccctgggcat
     1861 cgtctttccc cgaaagaacc agggcaaggg gtacgggagg gaggccctgg ggctcctaaa
     1921 ggggctcgtc ttcgacgacc tggggctcca tcggctggag ctcgaagtgc acgaggacaa
     1981 cgcccccgcc ctccggtgct accaaaaatg cggcttccgg gaagaggggc gccgccggga
     2041 acggcgcttt tccgggggac gcttctggga cgtggtcatc atggccctcc tggcgcccga
     2101 atatcgcgcc tcgaagatct cccgggggaa ggagtagccc ccccggtccc ttggccctgc
     2161 gggccttcga tctgtggcat aatgtctccc ggcgcaccgt cgccccccca tctcccatcg
     2221 ttttcaaagg aggtctgcgc ccatgcccca ccctagcgcc ccccgaagcc ggactaaagg
     2281 ggccgaagcc ccccccgccg ccctggtcct cgccgccgga cggtcgtctc gcatggggtg
     2341 cggcaagatg ctcctccccc tgggggactc caccgtgctg gcccaggcgg tgacccgtct
     2401 tcgggaaggg ggagcccatc ccgtgatggt ggtgacgggg caccatcggg aagcggtgga
     2461 ggccgaggcc cggcgcctcg ggtgccccac cgtggccaat cccgatcccg agggggacat
     2521 gttcagctcc ctccgggtgg gagtggcggc ccttcccccc aagaccccgg cggtgctggt
     2581 gcttcccgga gatactcccc tggtgcgccc ctccaccggc cgggccctca tcgccgccct
     2641 ccggggggcc gacgcggcga tccccacctt ccggcaacag ggagggcacc ccgtgcttct
     2701 ggggcgcgcc gccctcgcct ccctggccct ccgccccgcc cccgaggggt tacggagcgt
     2761 cctttcctct ccggagcttc gggtttgccg cgttcccgtc cccgacagcg ccattttgag
     2821 ggacgccgac acccccgagg acttcgccgc cctcacggcc cacgccgccc gggaaaagat
     2881 ccccaccgaa gaagaagtcg aggccctctt ggagctccat cggacgcctc aggaggtccg
     2941 gcgccacgcc caggccacgg cccgggccgc ccgggccctc gg
//
LOCUS       DAOOER010000139         2952 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_31915_length_2952_cov_5.734208, whole genome shotgun sequence.
ACCESSION   DAOOER010000139 DAOOER010000000
VERSION     DAOOER010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31915_length_2952_cov_5.734208"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PL162_09775"
     CDS             complement(<1..271)
                     /locus_tag="PL162_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92347.1"
                     /translation="MVVKKSSRATFNGLMAFSEIESLKARRTMHLIEPDKTDAREVLS
                     QINAVESNLTHIGADTVWGWGYRGQNIVVANIDTGVRYSHEALVNQ"
     gene            complement(316..654)
                     /locus_tag="PL162_09780"
     CDS             complement(316..654)
                     /locus_tag="PL162_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92348.1"
                     /translation="MPNERLAPPPRNIQRRFGESPARFTAWGLMLLLVLALSPSAWGA
                     DSEFKPSVEPALLRQIQKDDGAGYMIYFREKADLSAAQGMEWQARGRFVTAQLRETAE
                     RSQKSESGPI"
     gene            1373..2254
                     /locus_tag="PL162_09785"
     CDS             1373..2254
                     /locus_tag="PL162_09785"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQF92349.1"
                     /translation="MSPAVWGYFSAAGAALTWALAPVLYRLFIDRLPYSVINAVRSLG
                     YLTSAAAYLWAMEGWGSLAAPENLPLVGAIMVMGVVWFVVGDLLWFACLHRLGASVSS
                     VVFSSSPLLAVPSAWLFLGQVPSWGVVAAAFLIVSGLLVLNLRGQRAPVGTIRGGLLF
                     ALGAMVTGLSGTLTNAWFVQQMPVPQIEFWRAVAVTGVSWGILAATGEWGALRRASWR
                     CLGGIVVAGAFSLTVGNLFWSYALHYVTVGVATCINAIKPFVTATFAATFLGEKVTPR
                     LAWGMVLVVGGVLLLGR"
     gene            complement(2345..>2952)
                     /locus_tag="PL162_09790"
     CDS             complement(2345..>2952)
                     /locus_tag="PL162_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92350.1"
                     /translation="RELEEAAAAFSPRHREIGVHLGQGLLEKLPEELGGRTEDFFRRH
                     GFQMAEDEEALPPLVEGAILVAPDPLDPLSRPAETLVLALFAGAGAVVSGGGGMALIA
                     SGPLGLAIGAGLGLVAGWTLLTQGREWSRQKLEDLSLPPSVTRSLWSGKTLKALRDKF
                     LEDLTQNAETTLSSLAQELIHREKLRYEGEMGALSELQKLF"
BASE COUNT          445 a          867 c         1013 g          627 t
ORIGIN      
        1 actggttcac cagggcctcg tgggagtacc ggacccccgt gtcgatgttg gccaccacga
       61 tgttctggcc ccggtatccc catccccaca cggtgtccgc gccgatgtgg gtgaggttgg
      121 actccacggc gtttatctgg ctcaggacct cgcgggcgtc ggtcttgtcg ggctcgatga
      181 ggtgcatggt gcgtcgggcc ttgaggcttt cgatctccga gaacgccata aggccgttga
      241 aggtggcccg gctggatttt tttaccacca cgacgttgtc gatccagaag gaagtgtagg
      301 cggccccttg ttgggtcaga taggcccgga ctcgcttttt tggctccgct ccgccgtttc
      361 ccggagctgg gccgtgacga accgtccccg ggcctgccat tccatgccct gggcggcgga
      421 aaggtcggcc ttctcccgga agtagatcat gtaccccgcg ccgtcgtctt tctggatctg
      481 acgcaagagg gcgggttcca ccgagggctt gaactcggag tccgcgcccc aggccgaggg
      541 cgaaagggct aggacgagca aaagcatcag cccccacgcc gtgaaacggg caggggattc
      601 cccaaatctc cgctggatat tccggggagg tggggccagt cgttcgttgg gcatcttgac
      661 atccctcctt tgttaataac gagaacgaat tttccccgtt ccggcggatg atgtccggcc
      721 ggggcgggcc ctcggtcccc tgggaccggg ccaggacggt ggtagataaa gacgacctct
      781 ggggagacct ttgaccgaac cacctccttt tttggttctt gccggggcgc tccatgttcc
      841 ctcttcggcg cggtgatccg tggaccgagg cgaaggaagc cgatgattgc cccaacattt
      901 ggatttccgg acagcgtagc acagaaaatg cttttgtgcc gccggcgtaa ttttgctgtg
      961 gcgggacctt gacatggtca cttctgggcg gatgcagtcc agaatcacca cgtcttcgtc
     1021 gtggccctgc ttcgtccggg aagaaaagaa acgcctccac gcggggaaaa cgcccccacg
     1081 acgcgagtag ttcttaatcc gcgccccggc gatgacgagg cctgcggagg agagaagtcc
     1141 tgagcagaga ggcgtcttgg gaaaggaatt tttttcggct cggagaattt tgtggaaaag
     1201 atcatggaga tggcctgttt taggatccct tggcggaggg ctctccccgg ggcgtggcgg
     1261 ttgacggcct tggggggcgt gccatacttg cccccggacg gctccgggca tggagtcggt
     1321 cccgggggtg ggggggcgtg gatgtgatgc ggcgcgtttt tgaggcgtgg ccttgagccc
     1381 cgccgtatgg ggttattttt ccgccgccgg ggcggcgctg acgtgggccc tggcccctgt
     1441 gctctaccgc ctcttcatcg accgccttcc atacagtgtc atcaatgcgg tgcgcagttt
     1501 ggggtacttg actagcgcgg cggcgtatct ttgggcgatg gaagggtggg ggagcttggc
     1561 ggcccccgaa aacctgcccc tcgtgggggc catcatggtc atgggagtgg tgtggttcgt
     1621 ggtgggggat ctcctgtggt ttgcctgcct gcaccgcctg ggggcctcgg tctcctccgt
     1681 ggtattctct tcatctcccc ttttggcggt gcccagcgct tggctttttc tcggtcaggt
     1741 gccttcgtgg ggcgtggtgg cggcggcgtt cctcatcgtt tcgggtcttc tggtgttaaa
     1801 cctccgggga cagagggcgc cggtgggcac catccgaggg gggttgctct tcgccctggg
     1861 agccatggtc acggggctct cggggacctt gaccaacgca tggttcgtgc agcagatgcc
     1921 cgtccctcag attgaattct ggcgggcggt ggcggtgacg ggggtttcct ggggcatctt
     1981 ggcggcgacg ggagagtggg gggctctgag gcgggcgagc tggaggtgtt tgggaggtat
     2041 cgtggtcgcc ggggcgttta gtctcaccgt gggaaacctc ttttggagct acgctctcca
     2101 ctacgtgacg gtgggggtgg ccacttgtat caacgccatc aagcccttcg tcacggcgac
     2161 cttcgcggcg acctttctgg gagaaaaggt tacgcctcgc ctggcatggg gcatggtgct
     2221 ggtggtgggt ggggtgctac tcctcggtcg atgagccccg agacgacccc gcccgccagg
     2281 gccttgacgg aggtgaggat cggggacgaa ggttctcccg gggattgctg ccggtgatcg
     2341 gaccctagaa gagcttctgg agttccgaaa gggctcccat ctccccctcg tatcggagct
     2401 tttcccggtg tatcagctcc tgggccaggg acgaaagggt agtttcggcg ttttgcgtga
     2461 ggtcttccag gaatttatcg cgaagggcct ttagggtctt cccgctccag aggcttcgcg
     2521 tcacggacgg gggaagggaa aggtcttcca gcttctggcg agaccactcc cgcccctggg
     2581 tcagcagggt ccatccggcg actagcccta gccccgcccc tatcgccagc cccagagggc
     2641 cactggcgat cagagccatc cctccccctc cgctcaccac ggctcccgcc ccggcgaaga
     2701 gggccaatac cagggtttcc gcaggccgtg acaagggatc cagggggtcg ggggcgacca
     2761 ggatggcccc ttcgacgagg gggggtaggg cctcctcgtc ctccgccatc tggaatccat
     2821 ggcggcgaaa gaaatcttcg gtacggcccc ccaactcttc ggggagcttc tcgagaagcc
     2881 cttgtcccag gtggaccccg atctcccggt gccgggggga aaaggcggcg gcggcctcct
     2941 ccagctctcg gc
//
LOCUS       DAOOER010000140         2918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_32456_length_2918_cov_8.520084, whole genome shotgun sequence.
ACCESSION   DAOOER010000140 DAOOER010000000
VERSION     DAOOER010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32456_length_2918_cov_8.520084"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            149..1234
                     /locus_tag="PL162_09795"
     CDS             149..1234
                     /locus_tag="PL162_09795"
                     /inference="COORDINATES: protein motif:HMM:NF014094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HQF92351.1"
                     /translation="MSLLAFGERLCAGAKARTLRGRLLEDAVYWQLLHCEDVGEIVDR
                     LGTTPEYREVLEKVPREIHRSELEELLDLMLLRESGAFLGYLSGPRKAFLRAWVGLSE
                     ADGIKRALRRLAAGRGERDALRHRLEALPYCSFSVEGLLAADRPEAVREVLRPTPYFH
                     ALREPLRRFTEEGGGSLYGVEMALDGVALKRLYKSAQELPPGEREGVLSLAGSLVDLT
                     NVFWLYRGRFYFAQPPEEMLNRLLPVRHRVRIDLLRRLAGAGSGKDFWGELSDSPYRP
                     AMDQALSEELFVDRGMEAFLFRQATRLFAGGSPGFPMVVAYLFLRHYEVADLKTLVED
                     VRYGYTDRDAAVFLVRPILPGRGSVWR"
     gene            1225..>1637
                     /locus_tag="PL162_09800"
     CDS             1225..>1637
                     /locus_tag="PL162_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HQF92352.1"
                     /translation="MAVARMMALTVVGPKDETELVARHMVLRAGFQPIPLDYLVTDRG
                     LRAKISTATENPYDALVSRLATVWHAAGERLPEVAPVPLDRNFTLDSARIQVENAAHR
                     LELWARRSRELEEELEELEAARVMLEALESMEFTL"
     assembly_gap    1638..1737
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1738..>2918
                     /locus_tag="PL162_09805"
     CDS             <1738..>2918
                     /locus_tag="PL162_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HQF92353.1"
                     /translation="TVKKSLQGLEKAAKNFLGERREELEGLFCRLYTMQRVYELCKGR
                     GEIGDTYVFSGWVPADVLEELTRIMGEEAPRTTYFAEDSSTLRYGRSSVPTLLRNIPL
                     VRAFQDVVALYSLPGYDEIDPSFIVAVSFCLMFGFMFGDVGHGLLLALGARYLVKRRI
                     LRKNLGTVLTAASVFSMIFGVLYGSVFGLEGLFPPLWFSPSENTDKLLSIALGAGVGF
                     ITLGLALNMVTQYRQRNFGRLLFDGQGLAGLAFYWSAALVGFWVFSGHSLPLPGWAVG
                     GLFLALVLVMAFRDVLARVVLHERAHGEGGVVHAFEVAHGLLAFLSNTASFVRLAAFA
                     LNHVGLSMAVFMLGDMVRDLPGGLVLKGLLLVVGNLIIVGLEGLIVFIQTLRLEYYEF
                     F"
BASE COUNT          400 a          861 c         1005 g          552 t
ORIGIN      
        1 cctcgaccgg atgcgggagc gctttgacga aaacgtccag ggattggtgg ccaccctggt
       61 ggcggaggtg gcggagagct atcgccggaa ggccgaattc ctcggcgcct cagggggccc
      121 ccggccgggg gcctagaagg gactggccat gtcccttctc gccttcgggg agaggctttg
      181 cgcgggggcc aaggcccgaa ctctccgggg taggctcctg gaggacgcgg tgtactggca
      241 gcttctccac tgcgaggacg tgggggagat cgtggaccgc ctggggacca ctccggagta
      301 ccgcgaggtg ctggaaaaag ttccccggga gatccaccgc tccgagctgg aagagctcct
      361 ggacctcatg ctccttcggg agagcggggc ctttctgggg tacctttcgg ggccccggaa
      421 ggccttcctc cgggcctggg tggggctttc cgaggcggac ggcatcaaac gcgccctccg
      481 tcgcctggcg gcggggcggg gagaaaggga tgccctgcgc caccgccttg aggccctgcc
      541 ctattgctcc ttctccgtgg aggggcttct ggcggcggat cgccccgagg ccgtgcggga
      601 ggtcctgcgc cccacgccct actttcacgc cctgagggag cccctgaggc gtttcacgga
      661 ggagggaggc gggtcgctct acggcgtgga gatggccctg gacggggtgg cgctgaagag
      721 gctctataaa agcgcccagg agctcccccc cggagagcgg gagggggtgc tttccctggc
      781 gggatccctg gtggatctga cgaacgtctt ctggctctac cggggaaggt tctacttcgc
      841 ccagcccccg gaggagatgc tgaaccgcct tctccccgtg cgccaccggg tgaggatcga
      901 cctcctccga cgcctggcgg gggccgggag cgggaaggat ttctgggggg agctgtcgga
      961 ttccccctat cgtccggcca tggaccaggc cctttccgag gagctcttcg tcgaccgggg
     1021 gatggaggcc ttcctcttcc gccaggccac gaggctcttt gccggaggat ccccgggatt
     1081 tcccatggtg gtggcctatc tcttcttgcg ccactacgaa gtggcggatc tgaagacgct
     1141 ggtggaagac gtgcggtatg gttacacgga tcgggacgcg gcggtttttt tggtgcgccc
     1201 catccttccc gggaggggat cggtatggcg gtagcgcgga tgatggccct cacggtggtg
     1261 gggcccaagg atgaaacgga actggtggcg cgccacatgg tgcttcgggc gggctttcag
     1321 cccatccccc tggactactt ggtgacggat cgggggctcc gggccaaaat ttccaccgcc
     1381 acggaaaacc cctacgatgc cctggtgtcg cgcctggcca cggtgtggca cgccgccggg
     1441 gagaggctcc ccgaggtggc ccccgtgccc ttggaccgga acttcaccct ggattcggcc
     1501 cggatccagg tggaaaacgc cgcccatcgc ctggagctct gggcccggcg gagccgggag
     1561 ctggaggagg aactggagga gctggaggcc gcccgggtga tgctggaggc cctggaatcc
     1621 atggagttca ccctggannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnacc
     1741 gtgaaaaaat ccctccaggg gctggaaaaa gcggcaaaaa acttcctggg ggagcggcgg
     1801 gaggagctgg aggggctctt ctgtcgcctc tacaccatgc agcgggtcta cgagctctgc
     1861 aagggacggg gggagatcgg ggacacctac gtcttctcgg gatgggtccc ggcggacgtg
     1921 ctggaggagc tgacccgcat catgggcgaa gaggcccccc gcaccacgta tttcgccgag
     1981 gacagctcga cgctgcgcta tggccgatcc tccgtcccca ccttgctgcg caacatcccc
     2041 ctggtgcggg ccttccagga cgtggtggcg ctctatagcc tgccggggta cgacgagatc
     2101 gacccgtctt tcatcgtggc ggtgagtttt tgtctcatgt tcggcttcat gttcggcgac
     2161 gtggggcacg gcctgctttt agccctgggg gcgcgctacc tggtgaagcg ccgcatcctg
     2221 cgaaaaaatc tgggaacggt gctcaccgcc gcatcggtct tttccatgat cttcggcgtg
     2281 ctctacggca gcgtcttcgg cctggagggc cttttccctc ccctgtggtt ctctccctcg
     2341 gagaacacgg ataagctcct ttccatcgcc ctgggggcgg gggtggggtt catcaccctg
     2401 gggcttgccc tgaacatggt gacccaatat cgccagcgga acttcgggcg attgctcttc
     2461 gacggccagg ggctggcggg gctggcgttt tactggtcgg cggccctggt gggcttttgg
     2521 gtcttttccg gtcactccct tcccctgccg gggtgggcgg tgggggggct ctttcttgcc
     2581 ctcgtcctgg tgatggcctt tcgggacgtg ctggcccggg tggtgctgca cgagcgggcc
     2641 catggcgagg ggggggtggt ccacgccttc gaggtggccc acgggctttt ggcctttctc
     2701 agcaacaccg cctccttcgt ccggctggcg gccttcgccc tgaaccatgt ggggctttcc
     2761 atggcggtct tcatgctggg ggacatggtg cgcgaccttc ctggagggct ggtgttgaag
     2821 gggctcctgt tggtggtggg caacctcatc atcgtggggc tcgaagggct catcgtcttc
     2881 atccagaccc tgcgcctgga gtactacgag ttcttcag
//
LOCUS       DAOOER010000141         2903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_32684_length_2903_cov_6.025632, whole genome shotgun sequence.
ACCESSION   DAOOER010000141 DAOOER010000000
VERSION     DAOOER010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32684_length_2903_cov_6.025632"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="PL162_09810"
     CDS             complement(<1..343)
                     /locus_tag="PL162_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92354.1"
                     /translation="MAQVNVAEEIRSVEEWAKNAVQEARLEGVRIVAAARTEGDERVK
                     GAKQAAARWFRERLAAKESEANGMAGRMVEEGKGRAKALAESLGSRGQVVGNWIAEEV
                     MARYGRSARKEG"
     gene            complement(507..1037)
                     /gene="tsaE"
                     /locus_tag="PL162_09815"
     CDS             complement(507..1037)
                     /gene="tsaE"
                     /locus_tag="PL162_09815"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HQF92355.1"
                     /translation="MDSRTVFVETSSPEETHFLGEALARGSYPGLALLLCGDLGSGKT
                     EMVRGFMARWGYHQVRSPSFALVHEYPTAPPVVHVDLYRLSTLREEDGLGLEEYLLRG
                     SVLLVEWGEKWKTHPLQDLWEGDFSFLGDFSLSGISCRRRVALRAWGNRAQDALDRSL
                     AWFCAKGCSTMRNGGR"
     gene            complement(1040..1282)
                     /locus_tag="PL162_09820"
     CDS             complement(1040..1282)
                     /locus_tag="PL162_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92356.1"
                     /translation="MAMYRKRRSSEEWREEEERRSEKSRVRGLRYTLGSFTLSFLALV
                     AFKVVHWDRPMGPLFWVVGGLALGTGLMILSSRNKG"
     gene            complement(1266..2840)
                     /locus_tag="PL162_09825"
     CDS             complement(1266..2840)
                     /locus_tag="PL162_09825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HQF92357.1"
                     /translation="MLPLYDSDALRAVYALAEELFGLSGAVLMENAGHGAALILERLF
                     PGMDGVCLCCGGGNNGGDGLVLARHLAVRGFSIAAVHTTPEDRCSPLTLTNLRALRAL
                     GVPCFPSESLDDDALDTLLKNHPMAVDALLGTGSQGPPRGEVARVMEALGRFSGPILS
                     LDVPSGVNASDGTVPSFCVRATHTVTFLAPKVGLFVSPGAAACGSVHVVPIGVPVKAL
                     LSPHSGVYCGVFSDFSLPSRTWHQHKGSFGTVLVVGGSLSYPGAPVLAARAVLRSGGG
                     GVVLATPEAGDHRGVPLPEAVHCPLPGGEATEDHWSLVAPWFDRVDTVLVGPGLGRTP
                     GSGALARRLWSEAPVPLVADADALFALAESSPPPPAGVPRLVTPHEGEASRLLACSVE
                     AVRSRRLAAVRSLAARWAGVLLKGAWSLASDGRDVVSCSTAAPILAIPGSGDVLAGVV
                     ATLIAQGVPLLRAASLGSLWHAQSGALWASGGKDRGMRASELADALPGAGAAWTPPPG
                     GLSLAEGEKWSHGHVP"
BASE COUNT          615 a         1028 c          804 g          456 t
ORIGIN      
        1 aaccttcttt acgggcagaa cggccataac gcgccatcac ctcttccgcg atccaattac
       61 ccacgacttg cccccggctg cccaaagact ccgcgagggc cttagcccgc cccttacctt
      121 cttccaccat gcgccctgcc atgccattgg cttccgattc cttagccgcc agcctttccc
      181 ggaaccaccg ggccgccgcc tgtttggccc ccttgacacg ctcgtctcct tccgtacggg
      241 ctgccgccac gatgcgaaca ccctccaggc gagcctcttg gaccgcattt ttcgcccact
      301 cttccaccga cctaatctct tctgccacat tgacctgcgc catacgcccc atccctcctt
      361 tcgtggaaaa atcacgaaaa aaatccgcgc ctccacactc catacaatgc gccagatatc
      421 tgcgcccttt cattgtgcaa tctttcccaa gttgtgtcaa atgcatagaa tatttccgca
      481 agtaacgaca gcgtcgaggg gggctctcac ctcccaccgt ttctcatcgt cgagcaaccc
      541 tttgcgcaaa accacgccag tgaccgatcc aaggcatcct gggcccgatt cccccaggcc
      601 ctcagggcca ccctgcgacg gcaagagatc cccgacaaag aaaaatcccc caaaaaggaa
      661 aagtcccctt cccagaggtc ctggagaggg tgggtcttcc acttttctcc ccactccacc
      721 aatagcacgg acccccgcag gaggtactct tccaatccca gcccatcctc ttcccggagg
      781 gtcgaaagac gatagagatc gacgtgtacc accggaggcg ccgtggggta ctcgtgaacc
      841 agggcgaacg aagggctccg cacctgatgg tacccccatc gtgccatgaa tccccgcacc
      901 atttcggtct taccgctacc caggtctccg cacaacagga gggctaaccc cgggtaagat
      961 ccccgggcca aggcctctcc taaaaaatgc gtctcttccg gagaagaggt ctccacgaac
     1021 accgtcctag agtccacgac taccccttat tccgagagga caggatcatc aggcccgttc
     1081 ctagggcgag ccctcccacc acccaaaaaa ggggacccat ggggcgatcc cagtgcacga
     1141 ccttaaaagc gaccagggcg aggaacgata gggtaaaact ccccagggtg taacgcaacc
     1201 cccggacccg acttttctcc gaccgtcgtt cttcttcctc tcgccactct tcggaagaac
     1261 ggcgcttacg gtacatggcc atgggaccac ttctcccctt ccgccaaaga aaggcccccc
     1321 ggggggggag tccatgccgc cccggcgccg ggcaaggcat cggcgagctc ggaggccctc
     1381 attccccggt ctttcccccc cgaggcccat agggctccgc tttgggcatg ccataaacta
     1441 cctagagacg cggcccgcag gaggggcacg ccctgggcga tgagggtcgc caccaccccc
     1501 gccaagacat ctcccgatcc cggtatggcc aatatcggcg cggccgttga gcatgacacg
     1561 acatcgcgac cgtccgacgc cagggaccaa gctcccttga gtagcacccc ggcccatcgg
     1621 gccgcgaggc ttcggacggc ggcaagacgg cgagatcgca cggcctccac ggagcaagcc
     1681 aacaggcgcg aagcctcccc ttcgtgggga gtcaccaagc ggggcacccc ggcaggaggg
     1741 ggcggcgatg attccgccaa ggcgaagagg gcatctgcgt ccgccaccag gggcacgggg
     1801 gcctcggacc acagccgcct cgccaaggcc cccgatcccg gagtgcgacc gaggccaggg
     1861 ccgacgagga cggtatcgac acggtcaaac caaggggcca cgagggacca atggtcctcg
     1921 gtggcctcac ctcccggcaa gggacagtgt accgcctcgg gaagaggtac ccctcggtga
     1981 tcccccgcct cgggtgtggc cagcaccacc cctccgcccc ccgaccgcag gacggcgcgg
     2041 gccgccaaga ccggagctcc gggataggac aaagatcctc ccaccaccag cacggtgcca
     2101 aaagatccct tgtgttggtg ccatgtccga gaaggaaggg aaaagtcgga aaagactccg
     2161 cagtagaccc cagaatgagg agagaggagc gctttgacgg ggactccgat ggggacgacg
     2221 tgcacgctcc cgcacgccgc cgccccgggg ctcacgaaaa gccccacctt gggcgccaag
     2281 aacgtgaccg tgtgggtggc cctcacacag aacgagggca cggtgccatc tgaagcgttg
     2341 acgccactcg gcacgtccag ggataggatg gggccggaaa agcgccccaa ggcttccatg
     2401 acccgggcca cttcccctcg gggggggcct tgggatccgg tgcccaagag ggcatccacc
     2461 gccatcgggt ggttcttcaa gagggtatcg agggcatcgt cgtcaaggct ttctgaaggg
     2521 aaacaaggga cccccagggc ccgaagggcc cggagattgg ttagagttag aggggaacac
     2581 cgatcttccg gagttgtgtg cactgcggcg atggaaaaac cccggactgc caagtggcgg
     2641 gcgaggacta agccatctcc cccgttattt cctcctccgc aacacaggca caccccatcc
     2701 atcccgggga aaaggcgctc caggatcaaa gccgccccgt gccccgcatt ttccatgagc
     2761 accgccccag agaggccaaa gagttcctcc gccaaagcgt agacggcccg gagggcgtca
     2821 gagtcgtata ggggaagcat tttcgcctcc ttcaagcagc accaccgcca cggccatgtc
     2881 ctcttcgtga ctgacgctga cca
//
LOCUS       DAOOER010000142         2896 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_32798_length_2896_cov_9.239704, whole genome shotgun sequence.
ACCESSION   DAOOER010000142 DAOOER010000000
VERSION     DAOOER010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32798_length_2896_cov_9.239704"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="PL162_09830"
     CDS             complement(<1..752)
                     /locus_tag="PL162_09830"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQF92358.1"
                     /translation="MNLIRPFVRRPVMTSVFVVLMVFVGIYGYMDLGMALSPKVDLPL
                     VLVITSYPGAGPTEVETVLTKPIEDAVAQVEGIKKIESYSQEGSSVVLIWLEYDVDTA
                     GATLAVSNRVRAIQNTLPEDAYDPIIEKYDINAEPFLTLVVTSHLPPEQAYDLVEEKI
                     QRRLTQIQGLARAEIRGGVKREVQVYLDPARMIHFGLSVGTVMGAIAANTENDPSGHV
                     ARGSKETSLRVLGQFQTPQGLGEIRLPLGQGAS"
     gene            complement(768..>1169)
                     /locus_tag="PL162_09835"
     CDS             complement(768..>1169)
                     /locus_tag="PL162_09835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92359.1"
                     /translation="RQDPGADPATGLYKALVALEPGAPLRPGMVVEVDLLVDSRDRVV
                     AIPYEALRRENAKTFVFVLSGDRATRREVEVGEGQGGQVEILSRLYSGDVIIAEGTET
                     LFDGARVWIRPPGDPRESPEGPSELSPDGAP"
     assembly_gap    1170..1269
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1270..1733)
                     /locus_tag="PL162_09840"
     CDS             complement(<1270..1733)
                     /locus_tag="PL162_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92360.1"
                     /translation="MSEERRTLGQTGRLIFWAALFFGGMGFFAYRVVAIPPKAAESPS
                     IEEIRSEKGIPVVPYVVTADPWELWKRFYGPVRPGKEQRITSLLRERILAVHVDVNDA
                     VKKGDVLIELRAESQAADLSARKADYEDAQREYERQKALLAAGGASQQDVDRA"
     gene            complement(1737..>2896)
                     /locus_tag="PL162_09845"
     CDS             complement(1737..>2896)
                     /locus_tag="PL162_09845"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQF92361.1"
                     /translation="RHGEVAASAARLRAARATAEAVAASQRPTVALRGEGARIFREQE
                     GAASLYFSVQAPVDLWGRFDRICQQALLAYDLRAARHGELVNTLLTSAESLYWRAVLA
                     RADVVLYESLVRQREEDFRVAKKRHEVGSVAKLDVIRAEVQLESTRGELTSARTAYAD
                     YLVSLKALAGGTPVEPLSGDLAIPTRGLSEDVTFWLDRRPDLVALEVSRALARGEKEL
                     AALGMRPEISASLGYTLATDSDRSTPPEHDGALTLVVTVPLSDGGQTRSLTREKSWIE
                     EASSQDLRAARLRAEEELALVRNAWNRSASLVETKRREVELAGEELRIAALRYREGTG
                     TQLDLLDAQVKEQTARRGLLDATKDLYLASARFRGALGLYAAKFVPAESGD"
BASE COUNT          444 a          977 c          895 g          480 t
ORIGIN      
        1 gaggccccct gccccagggg gaggcggatt tcccccaatc cctggggggt ctggaactga
       61 cccaggacgc ggagggaggt ctctttgctc ccccgggcca cgtgccccga gggatcgttt
      121 tcggtgttgg cggcaatggc gcccatcacc gtccccaccg agaggccgaa gtggatcatt
      181 cgggcgggat ccaggtatac ctggacttct cgcttgactc ccccccggat ctcggcccgg
      241 gccaacccct ggatctgggt gaggcgccgc tggatcttct cttccaccag atcgtaggcc
      301 tgctccggcg gcagatggga ggtgaccacc aacgtcagga agggttcggc gttgatgtcg
      361 tatttttcga tgatggggtc gtaggcgtcc tcggggaggg tgttctggat cgcccgcacc
      421 cggttggaca ccgccagggt ggcgccggcg gtgtccacgt cgtactccag ccagatcagc
      481 accacggagg agccctcctg ggagtaactt tcgatctttt ttatgccctc cacctgggcc
      541 accgcgtcct cgatgggctt ggtgagcacc gtttccacct cggtggggcc cgctccggga
      601 taggaggtga tcaccagcac cagaggaagg tccaccttgg gggagagggc catgcccagg
      661 tccatatagc cgtaaatgcc cacgaagacc atgaggacca cgaagacgct ggtcatcacg
      721 gggcgacgca cgaaggggcg gatcaggttc atggcggccc gacctcccta gggagccccg
      781 tcggggctga gttccgaagg cccctcgggg ctctcccggg ggtccccggg ggggcggatc
      841 caaacccggg cgccgtcgaa gagcgtttcg gtcccttcgg cgatgatgac gtcgccggag
      901 tagagtcggg agaggatttc cacctgcccc ccttgtcctt cgcccacttc cacctctcgt
      961 cgggtcgccc ggtctccgga aaggacgaag acgaaggtct tggcgttctc ccggcgcagg
     1021 gcttcgtagg ggatggccac cacccggtcc cgggaatcca ccagcaggtc gacctccacg
     1081 accatgccgg ggcgcagggg agccccgggt tccaacgcca ccagggcctt gtaaagcccc
     1141 gtggcgggat ccgccccggg gtcctggcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnng ctcggtccac gtcctgctgg gacgctcccc cggcggccag aagggccttc
     1321 tgacgctcgt actcccgttg agcatcctcg tagtccgcct tccgggcgga aaggtccgcc
     1381 gcctgggatt cggccctcag ctcgatgagc acgtcccctt tcttcactgc gtcgttgaca
     1441 tccacatgca ccgccagaat gcgctcccgg aggagggagg tgatgcgctg ctccttcccg
     1501 gggcgcactg ggccgtaaaa gcgcttccag agctcccagg ggtcggccgt cacgacgtag
     1561 ggcaccacgg ggatgccctt ctcacttcgg atctcctcga tgctggggct ctccgccgcc
     1621 ttggggggga tcgccaccac ccgataggca aaaaatccca tgccgccgaa aaaaagcgcc
     1681 gcccaaaaga tcaggcgtcc ggtctgcccc agggtccttc tctcttcgct cattatttaa
     1741 tccccactct ccgccgggac aaatttcgcc gcatagagcc ccagggcccc ccgaaacctc
     1801 gccgacgcca ggtaaagatc cttcgtcgca tcgagcaacc cccggcgggc cgtctgctcc
     1861 ttcacctggg cgtccagaag atccaactgc gttcccgtgc cctcccggta tcgcaatgcg
     1921 gcgatgcgca gttcctctcc ggcgagctcc acttcgcgtc ttttggtctc caccagggaa
     1981 gccgaccggt tccaggcatt tcgcaccagg gccagctctt cctccgcccg gaggcgcgcc
     2041 gcccgaagat cctgggaaga ggcctcttcg atccagcttt tttcccgggt gaggctccga
     2101 gtttgccccc cgtcggagag gggcaccgtc accaccagcg tcagggcgcc gtcgtgttcc
     2161 gggggggtgc tccggtcgct gtccgtggcc agggtgtacc ccagggaggc ggatatctcg
     2221 gggcgcattc ccagggcggc cagctccttc tcaccccggg ccaaggcccg ggacacctcg
     2281 agggccacga gatcgggacg ccggtcgagc cagaaggtca cgtcctccga aaggccccgc
     2341 gtcgggatgg ccaggtcgcc ggagaggggc tccaccgggg tgccccccgc cagggccttc
     2401 aaagacacga ggtaatcggc gtaggccgtg cgggccgaag tgagctctcc ccgggtgctc
     2461 tccagctgga cctcggcccg gatgacgtcg agtttggcca ccgaccccac ttcatgacgt
     2521 ttttttgcca ctcgaaaatc ttcctcgcgc tgacgcacca gactttcgta gagcaccaca
     2581 tcggcccggg cgagcaccgc ccgccagtac aggctctccg ccgaggtgag caaggtgttg
     2641 accagttcgc catgacgcgc cgctcgcaga tcgtaggcca gcagggcctg ctggcagatc
     2701 cggtcaaacc tgccccagag gtcgacgggg gcctgcacag aaaaatacag cgacgccgcg
     2761 ccctcctgtt cgcggaagat ccgggcccct tctccccgaa gggccacggt ggggcgctgg
     2821 gacgccgcca cggcctccgc cgtcgcccgg gcggcccgga gacgggccgc cgacgccgcc
     2881 acttcgccgt ggcgtt
//
LOCUS       DAOOER010000143         2861 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_33345_length_2861_cov_8.021026, whole genome shotgun sequence.
ACCESSION   DAOOER010000143 DAOOER010000000
VERSION     DAOOER010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33345_length_2861_cov_8.021026"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1065
                     /locus_tag="PL162_09850"
     CDS             <1..1065
                     /locus_tag="PL162_09850"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQF92362.1"
                     /translation="DWLSQGPMVEVFEEKVASYLGVRHAVAFSSGTSALHGAYWAAGI
                     GPGDEVVTSPLTFAATSNGALYTGAEVRFSDVSPLTYCLCPHRAAEEVGPRVKAFAPV
                     SFAGYPADVPAFRSLAEKNGAVVIEDACHALGGERNGKKIGEEAHLTVFSFHPVKHIT
                     TGEGGMVVTESDDYADRLRRFRSHGIVKDPKQFLRPAPGPWHTEMQHLGYNYRLSDIQ
                     CALGASQMERLDDFLARRKQLAQWYGELLVGVPHVRPAPDHPGHAWHIYPLWVEPVLR
                     RQVFEGLREREIGVQVHYPLVSSHPYYVDRYGPQKGSFPEAERISGGEISLPMFPAME
                     DRDVDRVVDVLKNILESVAP"
     gene            complement(1082..1306)
                     /locus_tag="PL162_09855"
     CDS             complement(1082..1306)
                     /locus_tag="PL162_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HQF92363.1"
                     /translation="MHCIATFDTTHMALRFEKLCRADGVGARIVPIPRELSASCGLAC
                     RYPCDQESRVKELAASGHIDGVTYHRPKEG"
     gene            1455..1907
                     /locus_tag="PL162_09860"
     CDS             1455..1907
                     /locus_tag="PL162_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HQF92364.1"
                     /translation="MKRTQRWWGMLLVVAFVVALGAGTALAADDSKMGVLDPQKVLFN
                     HPNFKDTQEKIQKVTENKQNEAKMAIDAESDNKKKAEIYQKKREEAAMEEQKLMEPLF
                     KQVDTAIRTVATAKKLVIVFDKNVAFFGGVDITDDVIQELKKAAVKRK"
     gene            2047..>2861
                     /locus_tag="PL162_09865"
     CDS             2047..>2861
                     /locus_tag="PL162_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013049286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQF92365.1"
                     /translation="MGWRSLGSFFALNSSMVGLLTMVIFVGMGEKLAERFLPVYLMAL
                     GGGTLAVGLLNGLDNLLSALYSFPGGYVSDRLGTKRALVVFNLLAMGGFLVVILVPSW
                     QAVILGSVLFLSWTSLSLPATMSLVAKVLPSNKRTMGVSMHSLFRRIPMALGPVVGGL
                     CIDAFGEVAGVRWAFGGALVMAAVALGAQQRLIADDAADARKGSWSPPREMISLMDPA
                     LKNLLLADILVRFCEQIPYAFVVLWCMKDISSPVSAFQFGILTAVEMAVAMLI"
BASE COUNT          526 a          819 c          949 g          567 t
ORIGIN      
        1 gactggcttt cccagggccc catggtggag gtcttcgagg aaaaggtggc ctcctacctg
       61 ggggtgcggc acgccgtggc cttttccagc ggcacttcgg cgttgcacgg ggcctattgg
      121 gccgccggga tcggccccgg agacgaagtg gtcacttcgc cgctcacctt tgccgccacg
      181 agcaacggag ccctttatac cggggcggag gtgcgttttt ccgacgtgag ccccctcacc
      241 tactgtcttt gtccccatcg ggccgccgag gaggtcgggc cccgggtcaa ggcctttgcc
      301 cccgtgagct tcgcgggata ccccgccgac gtgcctgcct ttcgatccct ggcggaaaaa
      361 aacggtgcgg tggtcatcga agacgcgtgt cacgccctgg ggggcgagag aaacggcaaa
      421 aagatcggcg aggaggccca tctcacggtg ttcagcttcc acccggtgaa gcatatcacc
      481 acgggggaag gggggatggt ggtcacggaa agcgacgact atgcggatcg cctgcgacgt
      541 ttccggagcc atggcatcgt caaagaccct aagcagttcc tgcgccccgc gccggggccc
      601 tggcacaccg agatgcagca cctggggtat aactatcgcc tttcggacat ccaatgcgct
      661 ctgggggcga gccagatgga gcggctggac gattttttgg cccggaggaa gcaactggcc
      721 cagtggtacg gggagttact ggtgggggtg ccccacgtcc gccccgctcc ggaccatcca
      781 ggccacgcct ggcacatcta tcctctatgg gtggagcccg tgctacggcg ccaagttttc
      841 gaggggctcc gggagaggga aatcggcgtt caggtccact accccctcgt gtcgtcccac
      901 ccctactacg tggatcgcta cggtccccag aagggaagct tccccgaggc ggagcggatc
      961 tccgggggag agatttccct gcccatgttc ccggccatgg aggataggga cgtggaccgg
     1021 gtggtggacg tcctgaagaa tattctggaa agcgtggcgc cgtagggccc gcctccggga
     1081 ttcatccttc cttggggcga tggtaggtga ctccgtcgat gtgccccgag gccgccaact
     1141 ctttgacccg gctctcctgg tcgcaggggt aacggcaggc gaggccgcag ctggcggaga
     1201 gctcccgggg gatggggacg atgcgggcgc cgacaccgtc ggcgcggcag agcttttcaa
     1261 agcgcagggc catatgggtg gtgtcgaatg tggcgatgca gtgcacgaaa aacatctccc
     1321 ttcgaagggc ctccgctccc gaaaatccga gggagtcgac ggggcttccc gcaattatac
     1381 ctagacggaa aacctttggc acggtacaat ttcctggcgg gtgaatccat atcgaaggtt
     1441 taggaggtac gggaatgaag cggacgcagc gttggtgggg aatgttattg gtggtggctt
     1501 tcgtcgtggc gctgggggca ggcacggccc tggcggcgga tgattcgaag atgggggtgc
     1561 tggaccctca gaaggtgctt tttaaccacc ccaacttcaa agacacccag gagaagatcc
     1621 agaaggtcac ggaaaacaag cagaacgagg ccaagatggc catcgatgcc gaaagcgaca
     1681 acaagaaaaa ggccgagatt taccagaaaa agcgcgaaga ggccgccatg gaagagcaga
     1741 agctcatgga gcccctcttc aagcaagtgg acaccgccat tcgcacggtg gccacagcca
     1801 agaagctcgt catcgttttc gataagaacg tggccttctt cggcggcgtg gatatcacgg
     1861 atgacgtcat ccaggaactg aaaaaggccg cagtcaagag aaaataggcc ccgccttctc
     1921 aacaccataa cggctgtatc cgtggcgtga cctccccccg gaaccctttg gcgcttcgtt
     1981 gccgagggct tcggggggag gtttttttaa gggagcgaaa ttggggtccg gggagggtga
     2041 tgggggttgg ggtggaggtc gctggggtct tttttcgcgc tgaattccag catggtgggg
     2101 cttcttacca tggtgatctt cgtgggcatg ggagaaaagc ttgcggaacg ctttctcccg
     2161 gtctatctga tggccttggg ggggggcacg ctcgccgtgg gtctcttgaa cggcctggac
     2221 aaccttctgt cggccctcta ttccttccct ggagggtacg tttccgaccg gctggggacg
     2281 aaaagggctc tcgtggtctt taaccttttg gccatggggg ggttcctggt ggtgatcctc
     2341 gtgccgtcgt ggcaggcggt gatcctggga tcggtgctct tcctttcctg gacatctctg
     2401 tcgctccccg ccaccatgag cctggtggcc aaggtgctac cctcgaacaa gcgcaccatg
     2461 ggggtttcca tgcactcgct ctttcggcga atccccatgg ccctaggccc cgtggtgggg
     2521 gggctctgca tcgacgcctt cggcgaggtg gccggagtgc ggtgggcctt cggcggagcc
     2581 ctggttatgg ccgccgtggc gctgggggcc caacaacgac tcatagcgga cgacgccgcc
     2641 gatgcccgta agggttcctg gagccccccc agggagatga tatccctcat ggacccggca
     2701 ctaaaaaacc ttctcttggc cgacattttg gtgcgatttt gcgagcaaat accctatgcc
     2761 ttcgtggtgc tctggtgcat gaaggatatc tcttcccccg tgagcgcctt ccagttcggg
     2821 atcctgaccg ccgtggagat ggcggtggcc atgttgatct a
//
LOCUS       DAOOER010000144         2845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_33587_length_2845_cov_7.254480, whole genome shotgun sequence.
ACCESSION   DAOOER010000144 DAOOER010000000
VERSION     DAOOER010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33587_length_2845_cov_7.254480"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="PL162_09870"
     CDS             <1..1251
                     /locus_tag="PL162_09870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HQF92366.1"
                     /translation="SSAARILELVEKAAASKARTERFLSRFARWYTPAVVGAALLVAL
                     LPPLVMAGESFGAWAYRGLVLLVISCPCALVVSVPLGYFAGLGGAAHRGILVKGAAAL
                     EALAEARVVVFDKTGTLTDGVFRVRRQVPAEGFTEEDLLMWAAAAGAHSNHPLARAVR
                     RAWEEAGGEVREDGEEYEEIPGHGTGARVMGRKVLAGSLALLRLKGISGVPEGPGESE
                     ATAVHVAVEGVYAGVIEAGDGMKPGAPEALEELRRLGVERLVLLTGDGASPARRAGAQ
                     GGIDEIHAELRPEDKLAHLERLMEGGRERGSVVFVGDGVNDAPVLARADVGIAMGGSG
                     ADAAVESADVVLMTDDLSRVAEALRTARRTRRIVRENIVLVLAVKGVFLVLGALGYAT
                     MWGAVGADVGMALGAVLNARRAFR"
     gene            complement(1255..2307)
                     /locus_tag="PL162_09875"
     CDS             complement(1255..2307)
                     /locus_tag="PL162_09875"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HQF92367.1"
                     /translation="MVISAQFRLSGFYTRVSERIAALPLSPQGLLGAVILVAGGLSAL
                     LTNDVVCLAMTGVLGEGCLRRDLSPLPYALALACAANIGSAATLIGNPQNMLVGQVLH
                     LSFGGYFLVALVPTLLSLGALHLILVRIFRGRWQKAAPPLEPLESTPYHPWQAQKGML
                     VLALTVALFLGAPFPRELVALAAAGALLTSRTLASRATLGLVDWQLLVLFLGLFVVND
                     AFARAGGLEAIEEGLMASGLSLRSLPSLAAVVTALSNVVSNVPATMLLLPLMRDIPLA
                     GSTLALVSTFAGNLLLVGSIANIIVAGEAARLGVRLDWKVHARVGVPVTLVSLVIALG
                     WLLGLSLGDLPPGIRL"
     gene            complement(2301..2495)
                     /locus_tag="PL162_09880"
     CDS             complement(2301..2495)
                     /locus_tag="PL162_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92368.1"
                     /translation="MEESPFVYLVFGLVYAGMILGEFPRLAVDRTGIVLLGAIALVAS
                     EHGDFSGVDAPRSSCSSASW"
BASE COUNT          477 a          885 c         1091 g          392 t
ORIGIN      
        1 tcgtcggcgg cccgaatcct ggagctggtg gaaaaggcgg cggcctccaa ggcccggacg
       61 gagcgcttcc tgtcgcgctt cgcccgatgg tataccccgg ccgtggtggg ggcggcgctc
      121 ctggtggccc tcctccctcc cttggtgatg gcgggggaat ccttcggcgc ctgggcctac
      181 cggggattgg tccttttggt catatcctgc ccctgcgccc tcgtggtgag cgtgcccctg
      241 gggtatttcg cggggctggg gggggcggcc caccggggga tcctcgtgaa gggggccgcc
      301 gccctcgagg ccttggcgga ggcccgggtg gtggtcttcg acaagaccgg caccctcacc
      361 gacggggtct tccgggtgcg acgccaggtt cccgccgagg gattcaccga ggaagatctc
      421 ctgatgtggg cggcggcagc gggggcccac tcgaaccacc ccttggcccg ggcggtgcgg
      481 cgggcctggg aggaggccgg aggggaggtc cgggaggacg gggaggagta cgaggagatc
      541 cccggccacg gcaccggggc ccgggtgatg gggaggaagg tcttggcggg aagcctcgcc
      601 ctgttgcgcc tcaaggggat ttccggagtc cccgagggcc cgggggaaag cgaggccacg
      661 gcggtccacg tggcggtgga aggagtctat gccggggtca tcgaggcggg ggacgggatg
      721 aaaccgggcg cccccgaggc cctggaagag ctgcggcgcc tgggggtgga gcggctggtc
      781 ctgctcacgg gggatggggc ctcccccgcc cggcgcgccg gggcccaggg ggggatcgac
      841 gagatccacg ccgagctccg ccccgaggac aagctggccc acctggaacg cctcatggag
      901 ggggggcggg agcgggggag cgtggtcttc gtgggggacg gggtaaacga cgcccccgta
      961 ttggcccggg cggacgtggg gatcgccatg ggagggtcgg gggcggacgc cgccgtggag
     1021 agcgccgacg tggtgctcat gaccgacgac ctctcccggg tggccgaggc ccttcgcacc
     1081 gcccgccgca cccggcgcat cgtccgggag aacatcgtgc tggtgctggc ggtgaagggg
     1141 gtgttcctcg tcctgggggc cctggggtac gccaccatgt ggggtgccgt gggggccgat
     1201 gtgggcatgg ccctgggggc ggtgctcaac gcccgccggg cctttcggta ggagctagag
     1261 gcggatcccc gggggaaggt ccccgaggga tagccccagg agccatccca gggcgatgac
     1321 gagactgacc agcgtcacgg gaacgcccac ccgggcgtga accttccagt ccaggcgcac
     1381 tccgagccga gccgcctccc ccgccacgat gatgttggcg atgcttccca ccagcaggag
     1441 gttccccgcg aaggtgctca ccagggccag ggtgctgccc gccaggggga tgtcccgcat
     1501 cagaggcagg aggagcatgg tggcggggac gttggacacc acgttggaga gcgcggtgac
     1561 cacggcggcg agggagggga gggagcgcaa cgagagtccc gaggccatga gaccttcttc
     1621 gatggcctcc agcccccccg cccgggcgaa ggcgtcgttc accacgaaga gccccagaaa
     1681 gagcaccagt agctgccagt ccaccaatcc cagggtggcc cgggacgcca gggttcggct
     1741 ggtgagcagc gcccccgccg ccgccagggc caccaactcc cggggaaagg gagcccccag
     1801 aaagagggcc accgtgaggg ccagcaccag catccccttt tgggcctgcc agggatggta
     1861 gggggtgctc tccaggggct ccaggggggg ggcggccttt tgccagcgcc cccggaagat
     1921 ccgcacgagg atcaggtgga gggctcccag ggagagcagg gtgggaacca gggccaccag
     1981 aaaatacccc ccgaaggaga ggtggagcac ctgccccacg agcatgttct gggggttgcc
     2041 gatgagggtg gcggcggagc cgatgttcgc cgcgcaggcc aaggcgaggg cgtagggcag
     2101 gggggaaagg tcccggcgca gacatccctc gcccagcacc ccggtcatgg caagacacac
     2161 cacgtcgttg gtgaggaggg cggaaagccc tccggccacc agaatcaccg cccccaggag
     2221 cccctgggga gagaggggca gggcggcgat gcgctccgag acccgggtgt agaacccgct
     2281 caggcgaaac tgggcggaga tcaccatgaa gctgaagagc aggaggagcg tggggcgtcc
     2341 acgccggaga agtccccgtg ctccgaggcc accagggcga tggcccccag gagcacgatc
     2401 cccgtgcggt ccaccgcgag gcggggaaac tctcccagga tcatccccgc gtagaccagt
     2461 ccaaagacca gatagacgaa cgggctttct tccatgtgct tccccccttg gacaaaacgg
     2521 ccccattata gacactttgc cacgttttgc catggctttt ttggaatccg ggaaaatccg
     2581 aaaaagacgt ggacggggtc cccggttatt tgctaccatg acgtggcgat gttttttaaa
     2641 gaaccccggg ggcgcgaggg ggaatagggc gtcccccggc cccggggttt gggtcgtccc
     2701 gggaagatcg gtcggagacg ggcgcgcctc gccgcgcgga aacggagggg aaatcatgct
     2761 tttgcaggat tttaaagtag agaagtggct gaaccccctg gatcccatct gcacctacaa
     2821 tctgggggcc agctgcgtca aggcc
//
LOCUS       DAOOER010000145         2838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_33708_length_2838_cov_7.325189, whole genome shotgun sequence.
ACCESSION   DAOOER010000145 DAOOER010000000
VERSION     DAOOER010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33708_length_2838_cov_7.325189"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..2156)
                     /gene="flhA"
                     /locus_tag="PL162_09885"
     CDS             complement(27..2156)
                     /gene="flhA"
                     /locus_tag="PL162_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HQF92369.1"
                     /translation="MAVAQGDNGQLSIGMRFADLGMAILVILVVAMMIIPFPPGLLDI
                     FLSFNITFGVVVLLTTFYVRRALEISAFPTILLLATLYRLALNVSTTRLILLHGYAGD
                     VIEAFGNFVVGGNYVVGGVVFLILVIIQFIVITKGAERVAEVAARFTLDAMPGKQMAI
                     DADLNAGLIEEGEARRRRQEIQREADFYGAMDGASKFVKGDAIAGLIITVINILGGFA
                     IAVFQRGMEMEQALKVYSLLTVGDGLVGQIPSLLFSTATGIIVTRAAGESNLGQDIVQ
                     TLTAYHRPLWIGGGMLLSLAVVPGMPTVPFSLLGSLLLFLGYRSYREGMAAQERGAPP
                     PRGARAGGPPGERAGEGAPGRGEKPSTPPAPENVLPLLTVDPLEVEIGYALIPLVDPA
                     QGGDMLERIGTIRRQMALEFGLVVPPIRLRDNIQLKPTEYLILVKGGEVGRGELLPDH
                     YLAMNTTGSEDSLVGIPTTEPAFGLPAVWISPELRDQAEGAGYTVVDAPSVLATHLSE
                     TTKRYGADILTRQEVQKLVDMVKENHPAVVEEMLGAVGLGEIQKVLQNLIREQIPIRD
                     LVSIFEALADFGRASRSVDFLTERVRESLARVITLRLQDKAGLLTVGTLSPSWERRVK
                     ESLQGDLVQGWQINMDPKEMQKMVGAVAKFSEQMAFSGQMPVLLVHPDVRLVVRRIVE
                     GTLPHMAVVSYNEVAQGVQLKSVGMVE"
     gene            complement(2287..>2838)
                     /locus_tag="PL162_09890"
     CDS             complement(2287..>2838)
                     /locus_tag="PL162_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HQF92370.1"
                     /translation="VDTIHHALPLGAAQMGNAVWHLALKMALMLLVMAFFDYAYQRWE
                     FERSLRMSKKEIKDEYKQMEGDPKVKQRIRQRQREMARQRMMQDVPKADVVVTNPTTY
                     AVALQYDRAAMNAPTAVAKGRGVIARRIREIAEEHHVPIVENRPLARTLYDLVEVGEE
                     IPEHLYKAVAEVLAFVYKLKGKG"
BASE COUNT          540 a          998 c          791 g          509 t
ORIGIN      
        1 aaggtgatct gacgcaccac ccgcatttat tccaccatcc ccacggactt cagctgtact
       61 ccctgggcca cctcattata ggataccaca gccatatgtg gtaacgtccc ctcgacgatc
      121 ctcctcacca ccaggcgcac atcggggtgc accaaaagca cgggcatctg ccccgagaag
      181 gccatctgct ccgaaaactt cgccacggcc cccaccatct tctgcatttc cttgggatcc
      241 atgttgatct gccatccctg caccaggtcc ccttgcaggg attctttgac ccgacgctcc
      301 caggagggag aaagggtccc cacggtgagg agccccgcct tatcctggag ccgcagggtg
      361 atcacccggg ccagggactc ccggacccgc tccgtgagga aatccacgct acgggacgcc
      421 cggccgaagt cggccagcgc ctcgaagatg ctcaccaggt cgcggatggg gatctgctcc
      481 cggatgaggt tttggagcac cttctgaatt tccccgagcc ccacggcccc cagcatctcc
      541 tccaccacgg cggggtgatt ttcctttacc atatccacca gtttctgcac ttcctggcga
      601 gtcaggatat ccgccccgta gcgcttggtg gtctccgaaa ggtgggtcgc caacaccgaa
      661 ggggcatcca ccaccgtgta ccccgccccc tcggcctgat cccgcaattc cggcgaaatc
      721 cacaccgctg gcaagccaaa ggcgggctcc gtggtgggaa tccccaccaa gctatcctcc
      781 gatcccgtgg tattcatggc caagtagtga tccgggagca gctctccccg ccccacttcc
      841 cctcccttga ccaggatgag gtactccgtg ggtttcagct ggatgttatc cctcagccgg
      901 atggggggca ccaccagccc gaactccagg gccatctggc gccggatggt gccgatgcgt
      961 tccagcatat ctcccccctg ggcgggatcc accagaggga tgagggcgta gcctatctcc
     1021 acctccaacg gatccaccgt caggaggggc aggacgttct ccggagccgg aggggtcgag
     1081 ggcttttccc cccgccccgg cgccccctct ccagcccttt ctcccggggg ccccccggcc
     1141 cgggcccccc ggggcggggg ggccccccgc tcctgggccg ccatcccttc ccggtaagat
     1201 cgatagccca gaaagagcag cagggatccc agcagagaaa agggcacggt gggcatgccc
     1261 ggcaccacgg ccagggatag gagcatgcct cctccgatcc acaggggacg gtggtaggcg
     1321 gtgagcgtct ggacgatatc ctgccccagg ttagactccc ccgccgcccg ggtcacaatg
     1381 atacccgtgg cggtggagaa aagaagggac ggaatctgtc ccaccaggcc gtctcccacc
     1441 gtgaggaggc tatagacctt gagggcttgc tccatctcca tgccccgctg aaagaccgcg
     1501 atggcaaaac ctcccaggat gttgatcacc gtgatgatga gccccgcaat ggcgtctccc
     1561 ttgacgaact tggaggcgcc atccatggcc ccgtagaagt ccgcctcccg ctggatctcc
     1621 tgacgccttc ggcgggcctc accctcttcg atgagccccg cgttcagatc cgcgtcgatg
     1681 gccatctgct ttcccggcat ggcgtccagg gtaaaacggg ccgccacttc cgccacccgc
     1741 tccgcgccct tggtgatgac gatgaactgg atgatcacca ggatgaggaa caccacgccc
     1801 cccaccacgt agtttccccc caccacgaag ttgccgaagg cctcgatgac gtcccccgcg
     1861 tagccatgaa gcagaatgag ccgcgtggta gacacgttca gcgccagccg atacagcgta
     1921 gccaagagca agatcgtggg gaaggccgaa atctccaggg cccggcgcac gtaaaacgtg
     1981 gtaaggagca ccaccacccc gaaggtgatg ttaaacgaaa gaaatatatc caaaagcccc
     2041 ggcggaaagg ggatgatcat catggcgacc accaggatca ccaaaatggc catgcccaga
     2101 tccgcgaagc gcatgcctat ggaaagttgt ccgttatccc cctgagccac cgccatatcc
     2161 accgcccgtc ttcctcttca ggaagcctaa ttcgggaacg gcccgccccg ggcccctccg
     2221 cctgcgggtc aggcccgccc cccatcgctc ccaaaaaaag cctcgacgtt aaagggcgtg
     2281 gcgcccctag cccttcccct tgagtttata cacgaaagcc agcacctcgg ccactgcctt
     2341 gtagagatgt tcgggaattt cttctcccac ctccaccaga tcgtagagcg tgcgggccag
     2401 gggccggttt tccacgatgg ggacgtgatg ctcctcggcg atctctcgga tgcgccgggc
     2461 gatgacaccg cggcccttgg ccaccgccgt cggagcgttc atggccgccc ggtcgtactg
     2521 cagcgccaca gcgtaggtcg tggggttcgt caccaccaca tccgccttgg ggacatcctg
     2581 catcatcctc tggcgcgcca tctcgcgctg gcgctggcgg atgcgctgtt tgaccttggg
     2641 gtcgccctcc atctgcttgt actcgtcttt tatttccttc ttgctcatcc tgagggagcg
     2701 ctcaaactcc catcgctggt aggcgtagtc aaaaaatgcc atgacgagga gcatcaacgc
     2761 catcttcagc gcgaggtgcc acaccgcatt ccccatttgc gccgccccca ggggcagggc
     2821 gtggtggatc gtatccac
//
LOCUS       DAOOER010000146         2831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_33830_length_2831_cov_8.605908, whole genome shotgun sequence.
ACCESSION   DAOOER010000146 DAOOER010000000
VERSION     DAOOER010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33830_length_2831_cov_8.605908"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="PL162_09895"
     CDS             <1..361
                     /locus_tag="PL162_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92371.1"
                     /translation="HSPQVLSTVDRRSLRVLRLSDDGAWAESPRWQTRGVESADVLAS
                     VMGVNPIPRVEEAGWLADYRAIIGDGDPTSDEAVALRRKLLDHFGPDHPVMLDCDRLL
                     RFRDFRSAHSHSGREGR"
     gene            366..1001
                     /locus_tag="PL162_09900"
     CDS             366..1001
                     /locus_tag="PL162_09900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02646 family protein"
                     /protein_id="HQF92372.1"
                     /translation="MRKLDRTAVPAPSCLARYSHRHNRWEDLEGEDKEPIRDALRTVQ
                     NDLCAYCEGSLGDESHIEHFCRRRDFPDRIFTWENLFLSCSAQEHCGHYKDRSGASYN
                     PADLVKPDEDNPDEFLYFSSGGDVRPRGNIAAAQRRKAEETIRILNLNHGVLQAFRRR
                     ALATYRGLEPNILEALEGFEEAIRRQFIDEEIARTALDPYSAVIRHFFEAM"
     gene            1217..2641
                     /locus_tag="PL162_09905"
     CDS             1217..2641
                     /locus_tag="PL162_09905"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQF92373.1"
                     /translation="MGRWRVAGIVFATGLTSFLSTYLSSSVNLALKTIGGEFAVGPAD
                     LSLLASVYLLCSSLCIVPFGKLSDTYGPKRTLTFGCAFFTLSNLVVPLLARDFDSLLV
                     LRGLQGISSAFLVVSNTPIVTTSIPREYRTTALGFLSGLVYFGNSVGSFVGGALTESW
                     GWRSVFLSGAAGGAVAFVVLALVVPGDTARRDKAPRGIDVPGLLFYAVALLSLQSGAN
                     QLDRSWGPALLVLCLGALVFFVWRQFRAPNPLYDVRLALANPVFALANLAVFLNFLAT
                     YGSQYLLPLYLQCNRGLSPLEAGKVMLAQPLMQIFFSPLAGWIAGKIPPAFVASSGMG
                     MIAGALFVLTWLSATTPLWVIYGALMLIGVGISFFSAPNTSIIMEAVPPERRGMAAAS
                     NSLMRNLGMQCSIIFCGAAFLLLLGDVKGIPPEKYGDMLVTTRLCYGVFTAVCLGGMV
                     MSLKRRSLGALSSAAHEAPPGPHS"
BASE COUNT          469 a          842 c          874 g          646 t
ORIGIN      
        1 ccacagcccg caggtacttt ccaccgtcga ccggagatcc ctccgcgttc tccgcctgtc
       61 ggacgacggg gcctgggcgg agagtccccg ctggcagacc cggggcgtgg aaagcgccga
      121 cgtgctggcc tccgttatgg gcgtcaatcc cattccgcgg gtggaagagg ccgggtggct
      181 tgcggactac cgagcgatca tcggcgacgg cgatccgaca agcgacgaag cggtggcact
      241 gcggcgaaaa ctccttgatc acttcggtcc cgatcatccg gtgatgctcg actgcgaccg
      301 cctgctgcgc ttccgggatt ttcgctccgc ccactcccac tccgggcggg aagggcggtg
      361 atcccgtgcg caagctcgac cggacggcgg tccccgctcc aagctgtctt gcgcggtatt
      421 cccaccggca caatcgatgg gaggatctgg agggtgagga caaggagccg attcgcgatg
      481 ccctcaggac ggtgcagaac gacctttgcg cctattgtga aggctctctc ggagatgaga
      541 gccacattga gcatttttgt cgccggcggg acttccccga ccgcattttc acatgggaga
      601 acctcttcct ttcgtgcagt gcgcaagagc attgcggaca ctacaaggac cgatcaggcg
      661 cctcctacaa tccggcggat ttggtgaagc ccgacgagga caacccggac gagttcctct
      721 acttttcctc cggtggagac gtccgccctc ggggaaacat cgcggcagcc caacggcgca
      781 aagctgaaga gacgatccgc atcttgaacc taaatcatgg agtgcttcag gcctttcggc
      841 gccgcgctct ggcgacgtat agggggcttg aaccgaacat tctcgaggct ttggaaggct
      901 ttgaagaggc gattcggaga caattcatcg atgaggagat cgcccgtacg gcactcgacc
      961 cctacagcgc tgtcatccgg catttttttg aagcgatgtg agaagcttta gatgttttga
     1021 taaaattcat gacgtctgcc cagcataaaa ccgtttcacg agcactcttg ggtacttttt
     1081 gagagaacgg atcatccgca ctcgcagcag tgcggacgct ggagccattg aacggaggcg
     1141 tgaggcgtat cgtgtcaaaa agcgcatttg acccatgttt tgccacgccc cttgggggcg
     1201 ctacgggagg tttctgatgg gacggtggcg cgtcgcgggc atcgtttttg ccacggggct
     1261 cacgtccttt ctgtccacgt atttgtcctc gtcggtgaac ctggccctga agaccatcgg
     1321 aggggaattc gccgtggggc cggcggatct cagtctcctc gcctcggtct atcttctgtg
     1381 ctccagtctc tgcatcgtgc ccttcggcaa actctccgac acctacggtc cgaaacggac
     1441 cctcaccttc ggttgcgctt ttttcaccct ttccaatctg gtggtgcccc ttctggctcg
     1501 ggactttgac tcgctcttgg tgctccgggg gctccagggc atttcttcgg cctttctcgt
     1561 ggtgtccaat acgcccatcg tcaccacttc cattccccgg gagtaccgca ccacggcgct
     1621 gggcttcctc tcgggattgg tatattttgg aaactccgtg gggagttttg tcgggggagc
     1681 cctcacagaa agctggggat ggcggagcgt gtttctgagc ggagcggcgg ggggagcggt
     1741 ggccttcgtg gtgcttgccc tggtggtgcc cggggacacg gcccggcgtg acaaagcgcc
     1801 ccggggcatc gatgtgccgg gacttctctt ctacgccgtg gcgctcctga gccttcaatc
     1861 cggggcgaat cagctcgacc gatcctgggg ccccgcgctg ctggtactat gcctcggcgc
     1921 cctggtgttt ttcgtgtggc gtcagttccg agcgccgaat cctctctacg acgtgcgcct
     1981 tgcccttgcc aatccggtgt tcgccctggc gaatctggcg gtgttcctga actttcttgc
     2041 cacctatggt tcccagtacc tgctgcccct ctatctccag tgcaaccggg ggctttctcc
     2101 tctggaggcg gggaaggtga tgctggccca acctctcatg cagattttct tttctcctct
     2161 ggcggggtgg atcgcgggga aaatcccccc cgctttcgtg gcatcgtcgg ggatgggcat
     2221 gatcgccggg gcgctctttg tcctgacctg gctttcggcc accacacccc tttgggtgat
     2281 ctacggggcg ctgatgctca tcggcgtggg catttcgttc ttttccgcgc caaacaccag
     2341 catcatcatg gaagcggtgc cgccggaaag gcgcggcatg gccgccgcat ccaattctct
     2401 catgcggaat ttgggcatgc agtgcagcat cattttctgc ggcgcggcct ttttgttgtt
     2461 gctgggggat gtgaagggca ttccgccgga aaagtacggg gatatgctcg tcaccacccg
     2521 gctgtgctac ggcgtcttca ccgccgtgtg tttgggcggc atggtgatgt ccctcaagcg
     2581 aagatctttg ggggcgctgt cttcggcggc tcacgaagcc cctccaggtc cccattcctg
     2641 agcaagggga gttctccggg gcgctttggg ggaacccggc ggtgcggtcc ctggggcaaa
     2701 ggggcaagcg tcgatggggc cctatttacc gacgacgggc gacggccttt gaagaggagt
     2761 ttgtgaatgt caacatacag tagcaatgtc tatttttttg acgtcttttg ggccaaagaa
     2821 atgtcataac g
//
LOCUS       DAOOER010000147         2826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_33929_length_2826_cov_5.571635, whole genome shotgun sequence.
ACCESSION   DAOOER010000147 DAOOER010000000
VERSION     DAOOER010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33929_length_2826_cov_5.571635"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PL162_09910"
     CDS             complement(<1..655)
                     /locus_tag="PL162_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92374.1"
                     /translation="MYVRTKTSKLSQYKAVQIVEAYVAPGGKRRQRIVQHLGVARDDQ
                     QIEELVALGKRLIPELEARAREEKALREGQGLLFEIVPEDFERPVTDETQVKVKNLRN
                     MEHVTEGPFEVWGEVFSRLGLEGIFGTGVHDIGSTNLLKLCLTAKLCDGGSKLRATEW
                     INETLGLKLGKDRIYRMMDRLAPRIDQVKERGFLCGRHLVQGDVSLLLFDVTTLYFES
                     "
     gene            complement(1059..1889)
                     /gene="amrB"
                     /locus_tag="PL162_09915"
     CDS             complement(1059..1889)
                     /gene="amrB"
                     /locus_tag="PL162_09915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HQF92375.1"
                     /translation="MRSIALVAGVLVGVFLFPSGAQALGCPPLPPRVHRALEGPRDPR
                     DSGVEGRVLGGLTPHHDLAGELMVRFYEELRRGAPHPRRILLIAPDHHRAGRGAVTFC
                     GAPWETDRGRVPADEEGVAMLEKHGVARRDDALFPREHGITVHLPLLATFFPGVPVLP
                     LVVRSSVSDLELLALRKVLSPLMDSGLVILSMDLSHGKSSAQALEEDRRTLSVLSELR
                     WKELRGLDLDCPRGGALFLALLEERGARRGVVRDHATAASFGEPPEVPGTSYATVLFF
                     "
     gene            complement(1886..>2826)
                     /locus_tag="PL162_09920"
     CDS             complement(1886..>2826)
                     /locus_tag="PL162_09920"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HQF92376.1"
                     /translation="LAVLGSSAALRETKAFETFRGYAENREALRGRWKAFPQDPLGDT
                     IYADVLRLYATYFEPTEGGQFFAASPAWEYRKLVSAAASWAELKHDTILYGEQSGAEM
                     GDGGDVWYAPDFLCPEPRGYVEPEPRLFRGLVAAAGRIGQFLEQAEMAEGEYAEKLST
                     FAALMERLAHLAELEVTGAPLGDEEYRLIREFPAELTRELLLPADQLGLYPPLLPEVQ
                     DRLRMALVADVASDFAAGRALEVGVGAPRYLYVFVNDRWGGPRVTRGAVCSFYAFDRP
                     LEEGRLTDEEWKKMVYQGDPAALDRLRPDWTRVPEQ"
BASE COUNT          541 a          961 c          847 g          477 t
ORIGIN      
        1 aactctcaaa gtaaagggtg gtgacatcaa agagcagcag ggagacgtcc ccctgtacca
       61 gatgacgccc gcacaaaaag ccccgctctt tcacctggtc aatccggggg gccagccggt
      121 ccatcatccg gtagatccta tctttgccca gtttcagccc caaggtctcg ttgatccatt
      181 ccgttgcccg aagcttgctc cctccgtcgc agagcttcgc cgtcaggcag agcttcagca
      241 gattcgtcga cccgatgtcg tggacccccg tgccaaagat cccttcgagt cccagcctcg
      301 agaaaacctc tccccagacc tcgaaaggcc cttccgtcac gtgctccatg ttgcgaagat
      361 tcttcacctt cacttgcgtt tcgtcggtga cgggacgctc gaaatcctcg ggaacgatct
      421 cgaaaagaag cccctgcccc tcccgcaggg ccttttcttc ccgagcccga gcctccagct
      481 ccgggatgag tcgcttcccg agggccacga gctcctcaat ctgttggtcg tcacgggcca
      541 cgccgaggtg ctgcacgatg cgctgacgcc gcttgcctcc cggcgcgacg taggcctcga
      601 cgatctgaac cgctttgtat tggctaagct tggaggtttt cgttctgaca tacatgggga
      661 tagagtagca cgggggggag ctcttgtcaa gaaatattcg taggcactac ctttcggttt
      721 tttccgcccc caacgacaaa aaagaagggc tcatcgccgt gagcccttgc aaagactaga
      781 tatgtgtttt tgatatatgc cgatctttaa tcgagtgggt tgggggaatg cgaaagtcgg
      841 gaaaaaaccc tgagtccttg ccatcactag ctttctacaa agcgttaggc actacgagat
      901 gttctcctcc tccgggagaa agctcaccat cgtagcccac attccgagac aaagccggct
      961 ggaagccggc gctcccagca aaagaaagcg aaagtcagga aaaattcccc ccaaggcgtt
     1021 gcctgaggtc gtccggaggg gaacgccctg ggccggggtc agaaaaagag caccgtggcg
     1081 tagctcgtcc ccggcacctc gggaggctcc ccaaaggagg ccgccgtggc gtggtcccgc
     1141 accacccctc gccgggcccc ccgctcctcc aggagcgcca gaaagagcgc cccccctcgg
     1201 gggcagtcca gatccaatcc ccggagctct ttccaccgga gctccgagag cacggagagg
     1261 gtgcgacggt cttcctccag cgcttgggcg gagctttttc cgtgggagag atccatgctg
     1321 aggatcacca acccggaatc catcagggga gagaggacct tgcgcagtgc caggagctcc
     1381 agatcggaga cggagctccg caccaccagg ggtaacaccg gcaccccggg gaagaacgtc
     1441 gccaaaaggg gtaggtgcac cgtgatgcca tgctctcgcg gaaaaagggc gtcgtctcgc
     1501 ctcgcgacgc cgtgcttttc cagcatcgcc actccttctt cgtcggcggg cacccttccc
     1561 cgatcggtct cccagggggc cccacaaaac gtcacggctc cccgccccgc ccggtggtgg
     1621 tcgggggcga tgaggaggat tcggcgaggg tggggcgccc cccgccgcag ttcttcgtaa
     1681 aaccgtacca tgagctcccc cgccaggtca tggtggggag tgagcccccc caggaccctt
     1741 ccctccactc cggaatctcg gggatccctg gggccctcga gggcccgatg gaccctgggg
     1801 ggaagcggag gacaccccag ggcctgggcc ccggaaggga acaaaaagac ccccaccagg
     1861 acccccgcca ccaaggcgat ggatctcact gctccggcac ccgggtccaa tcggggcgca
     1921 ggcggtccag agcggcgggg tctccctggt agaccatctt tttccactcc tcgtcggtga
     1981 ggcgcccctc ttccaggggg cggtcaaagg cgtaaaacga gcacaccgct cctcgagtca
     2041 cccggggtcc tccccaccga tcattgacga agacgtacag atatcggggg gccccgacgc
     2101 ccacctccag ggccctcccg gccgcaaaat ccgaggccac gtccgccacc aacgccatgc
     2161 ggaggcgatc ctgcacctcg ggaagcaggg gggggtagag gcccagctgg tccgccggga
     2221 ggagcagctc ccgggtcagt tccgccggga actcccggat gaggcggtac tcctcatctc
     2281 ccaggggggc ccccgtcacc tccagctccg ccaagtgggc caggcgctcc atgagggcgg
     2341 cgaaggtgga gagcttttcg gcgtactccc cttcggccat ctccgcctgc tccaagaact
     2401 gccctatccg ccctgcggcg gcgaccaacc cccggaaaag tcggggctcg ggttccacgt
     2461 agccccgggg ttcgggacag agaaagtccg gggcgtacca gacatctccc ccatctccca
     2521 tttccgcccc ggactgctcg ccgtagagga tcgtgtcgtg cttcagctcc gcccaggaag
     2581 cggcggcgga aacaagtttg cggtactccc acgccggaga ggcagcgaaa aattgccccc
     2641 cctcggtggg ctcgaagtac gtggcgtaga gccgcagcac atcggcgtag atcgtgtccc
     2701 ccaggggatc ctgggggaag gccttccacc gcccccgaag ggcctcccgg ttctcggcgt
     2761 acccccggaa ggtctcgaag gcctttgtct cccggagggc cgccgaggat ccaagcaccg
     2821 ccaggg
//
LOCUS       DAOOER010000148         2576 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_38623_length_2576_cov_6.283221, whole genome shotgun sequence.
ACCESSION   DAOOER010000148 DAOOER010000000
VERSION     DAOOER010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38623_length_2576_cov_6.283221"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..1217
                     /locus_tag="PL162_09925"
     CDS             114..1217
                     /locus_tag="PL162_09925"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HQF92377.1"
                     /translation="MFLSGNGAEGAPRGAFSPSVTSEPFGVTQKGEAVRRHTLTGGGL
                     TLRCLDYGGAVQALEAPDRQGRQESVVLGYDTLEEYEHDPVWCGVLCGPVAGRVAGAS
                     FELGGRRYAMEANDGTACLHGGSRGFSRRVWHGKPFVEGDTAGVRLSLDLSPEEDPFP
                     GPRSVSATYTLSPGGVFSLLWEATSPEAMVLAPTFHGYFNLGGPRCPSVEGHRLRLDA
                     ERYMPLDEAHLPQGPEPVEGTPFDFRTPRLLGDFFRQEHPQILRGRGSDHAFVFRPAP
                     GGPQGVLEHPESGRRMELFSHEPCAVFYSGGGLASVAHFSEQRRGAPHLAMALEPQWY
                     PNALGLPELPPPVHPPGTSWKSRSTWRFSVMPL"
     gene            complement(1274..2155)
                     /locus_tag="PL162_09930"
     CDS             complement(1274..2155)
                     /locus_tag="PL162_09930"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQF92378.1"
                     /translation="MGEGMGERGGIWRKVGLGMVGLLLAVVLSWGAEARGAERLNDKF
                     LVHLPEAAREGDFSFAYIGDVQINFSLFEQALQRMGEDPQVAFLIVGGDTMDKASDEG
                     FKAFLRRVKNKPFPVVAVPGNHDLYGDPGATLFRQYIGDGVTSFVVGKSQFILLQNTT
                     GNIPKDVKKSFVALLDSYAANPSLRHLFVVMHVPPFDPRTNSPGHSMNAREARKFFAL
                     LEPLATRHRTVTVMSSHVHGCFFRDHGPVRVVVSGGGGGGLYGKGEEFFHHYMKVHVR
                     GDRVSFVPVRLEKKKKP"
     gene            2353..>2576
                     /locus_tag="PL162_09935"
     CDS             2353..>2576
                     /locus_tag="PL162_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92379.1"
                     /translation="MVPKKHSEAFHRIPWHRYFLGILLIGLLGGIADKAPGEELSRDW
                     SSRIAALEEEIDTARRSETLEFSSRVGVPLG"
BASE COUNT          431 a          879 c          800 g          466 t
ORIGIN      
        1 aggtgcgccc ccgcccccac gcctcggagc tctacacccg ccccgggggc tttctagagc
       61 tctacgggcg ggaagagcga cgggcgctgg aagccctggg agaggcctag gccatgtttt
      121 tgtccggaaa tggggccgaa ggggcccccc ggggggcctt ctccccttcg gtgacgtcgg
      181 aaccctttgg cgtcacccaa aagggggagg cggtgcgccg ccataccctc accggcgggg
      241 ggctgacgtt gcgttgcctg gattacggcg gcgcggtcca ggctctggag gcgccagatc
      301 gccagggacg gcaggaaagc gtggtgctgg ggtacgacac cctggaggaa tacgaacatg
      361 acccggtctg gtgcggggtg ctctgcggcc ccgtggcggg gcgcgtcgcc ggggcgtcct
      421 tcgagctggg ggggcgacgg tacgccatgg aagccaacga cggcaccgcc tgcctccacg
      481 gcggatcccg gggcttttcc cggcgggtct ggcacggcaa acccttcgtc gaaggggaca
      541 cggcgggggt gcgcctttcc cttgaccttt ctccggagga ggacccgttc ccggggcccc
      601 gctccgtcag cgccacctac accctctctc ccggaggggt cttttccctg ctctgggaag
      661 cgacaagtcc ggaagccatg gtgctggccc ccacatttca cggctacttc aacctcgggg
      721 gcccccggtg tccctcggtg gaggggcacc ggctccggct cgacgcggaa cgctacatgc
      781 ccctggacga agcccacctg ccccaggggc cggaacccgt ggagggcacc cccttcgact
      841 ttcgcacccc ccgtctcctc ggggattttt tccgccagga gcacccccag atcctccggg
      901 ggagggggag cgaccacgcc tttgtctttc gccccgcccc cggggggccc cagggggtgc
      961 tggagcaccc ggagagcggg cgccgcatgg agcttttttc ccatgaaccc tgcgcggtgt
     1021 tttactccgg aggaggactg gcgtcggtgg cccacttttc cgagcagagg cgaggggcgc
     1081 cgcacctcgc tatggccctg gaaccccagt ggtatcccaa cgccctgggg cttcccgagc
     1141 tccctccgcc ggtacacccc ccgggcacgt cctggaaaag ccgctccacc tggcgttttt
     1201 ccgtcatgcc cctttagctc aagccccaag gcgccgcccc ttccccctac gggatccccc
     1261 cgaaggggta gcgctagggc tttttctttt tctccaggcg caccggcacg aaggacaccc
     1321 gatctccccg gacgtgcacc ttcatgtagt ggtgaaagaa ctcctccccc ttgccgtaga
     1381 gcccccctcc cccgcctccg ctcaccacca cccgcacggg gccgtggtct cgaaagaagc
     1441 agccgtgcac gtgggagctc atgacggtga cggtgcgatg gcgggtcgcc aggggctcca
     1501 gcagggcgaa gaacttgcgg gcctcccggg cgttcatgct gtgcccgggg gaattggtgc
     1561 ggggatcgaa ggggggcacg tgcatgacca caaagagatg gcggagggac gggttagcgg
     1621 cgtaggagtc caacagggcg acaaaacttt ttttcacgtc ctttgggata tttcccgtgg
     1681 tattttgcaa cagaataaac tgactcttgc ccaccacgaa cgaagtcacc ccgtccccga
     1741 tgtactgccg aaagagggtg gcgccggggt caccgtagag gtcgtgattc cccggcaccg
     1801 ccaccacagg aaagggcttg ttcttcaccc ggcggagaaa ggccttgaaa ccctcgtcgc
     1861 tggctttgtc catggtgtcc ccccccacga tgagaaaggc cacctggggg tcttcgccca
     1921 tccgttgtag cgcctgctcg aagagactga agtttatctg cacgtccccg atgtaggcga
     1981 aggaaaagtc cccttcgcgg gccgcctcgg ggaggtggac caggaacttg tcgttaaggc
     2041 gttccgcccc ccgggcctcg gcgccccagg acaggaccac cgccaaaagc agccccacca
     2101 tcccgaggcc caccttgcgc catatgccgc cacgctctcc catgccttca cccatacctc
     2161 cacgcctcct ctttcatccg atctctctta cgatctccct ggagccctca tcccatgaat
     2221 ttcgctcttc acatctgcga agccagggcc taaaagcggg gaaggtcatt cttccccgct
     2281 tgtttttcgg caaaaaacgc ctatgctggc aagaaaactt aggctcatca tcgcctccgg
     2341 gaggggtagc tcgtggtgcc aaaaaaacat tccgaggcct tccatcgtat cccatggcac
     2401 cggtactttc tggggatctt gctcataggt ctcctggggg gaatcgcgga caaagctcca
     2461 ggggaagagc tctcccggga ctggtcctcc cgcatcgctg ccctagaaga agagatcgac
     2521 accgcccgtc gctccgagac tttggaattt tcatcccgag tgggggtgcc cttggg
//
LOCUS       DAOOER010000149         2534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_39576_length_2534_cov_4.895522, whole genome shotgun sequence.
ACCESSION   DAOOER010000149 DAOOER010000000
VERSION     DAOOER010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39576_length_2534_cov_4.895522"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="PL162_09940"
     CDS             <1..854
                     /locus_tag="PL162_09940"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQF92380.1"
                     /translation="GDGILAGGCSPDPREREMFARALDLPVFETPEDLLRVVRPRVVS
                     ICSPTSFHTSHVMCCLEAGVPLIWLEKPPAASLAELDALRREARGRARVLVHYHRRYA
                     PFFRLLRREIRRGSLGPFRGGEVVYSRGLASNGIHFVDLVLFLWDVAGVPEISFRHVS
                     AFGEDYPTFALVVGGGEILFRGVDVPGHLFEGSFSFDDARVRFADGGERGGVEMRREN
                     PRYPGFFHYHPEASPLDGAGLRGCLAAALEDLFAAFREGRDPESNLDTSWGAEAVVEA
                     LRGGKTR"
     gene            851..1900
                     /locus_tag="PL162_09945"
     CDS             851..1900
                     /locus_tag="PL162_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92381.1"
                     /translation="MKRWVFAGGEIGGARALLPVLEAATAGGIPFSVVPFGVLGEEGE
                     RRGWLGECSCREPQGVWGGDGVVGLVFSTPSREDPVPLCWARSARRAGLPVVFLLDNW
                     MNYRRRLEMDGLPVLIPDRYAVMDALAREEAVADGIPPEVIRVTGHPALALLARERAR
                     GGGGERGLVVFLSEPVSQDQGEGPENPSFRGYTQEGVFRRLGALLRRTAEPWTLAVQP
                     HPREDPQRWHDLFRSVWTGEGGVGLFSGGREAVWAARGVAGMASLLLHEAWLLGKPVL
                     SLQPGLRNPQFRGVLRRKEIFGVTDTKEGSAAERGWLRALEKGNPVPRPELEEHAGAP
                     DRVLAIMRELTEGIA"
     gene            1897..>2534
                     /locus_tag="PL162_09950"
     CDS             1897..>2534
                     /locus_tag="PL162_09950"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HQF92382.1"
                     /translation="MSTLALLGGTPVRTEKFPGYRVMGQEEKDATARVIDSGVLSGFI
                     AGWHENFWGGPEVRALEEEWAAHFRVKHAIAVNSATSGLMAAVGAVGVEPGDEVIVPP
                     YTMSATAVAPLVWNAVPVFADLEPRGFCLDPVSVEARITPRTRALMAVDIFGHPYDAD
                     AINALARKYGLAVIEDCAQAPGATYRGRFAGTLGNLGVFSLNFHKHIHCGEGG"
BASE COUNT          360 a          699 c          984 g          491 t
ORIGIN      
        1 ggggtgacgg cattctcgcc ggggggtgct ctcccgaccc ccgggagcgg gaaatgtttg
       61 ctcgggcgct ggaccttcct gttttcgaaa cgccggagga tttgctgcgc gttgtccgcc
      121 cccgggtggt gagcatctgc tctccgacgt cttttcatac atcccatgtg atgtgttgcc
      181 tggaggcggg agttcccctg atctggcttg aaaagccccc cgcggcctct ttggcggagc
      241 tggacgccct ccggcgggag gcccggggaa gggcccgggt gctggtgcac taccaccggc
      301 gctacgcccc cttctttcgt ctgcttcggc gggagatccg ccgggggagt ttgggcccct
      361 tccggggagg tgaggtggtc tattcccggg ggctcgcgtc caacggcatc cacttcgtgg
      421 atctggtgct ctttctctgg gatgtggcgg gggtgccgga aatttctttc cggcacgtct
      481 cggcctttgg agaggattac cccacctttg ccctggtcgt ggggggaggg gagatccttt
      541 tccggggggt ggacgtgccg gggcatctct tcgaggggag tttctccttc gacgacgccc
      601 gggtgcgttt tgcagacgga ggagagcgcg gcggtgtgga gatgcggcgg gaaaatcccc
      661 gctatccggg gtttttccac taccatcccg aggcgtcgcc cctggacggt gcggggctcc
      721 gggggtgtct ggcggcggcg ctggaggatc ttttcgcggc ctttcgagag ggtcgggatc
      781 cggagagcaa tctggatacc agctggggcg ccgaggcggt ggtggaggcg ttgcgggggg
      841 ggaagacgcg gtgaaacggt gggtttttgc gggcggcgag atcggagggg cccgggcgtt
      901 gctccccgtg ttggaggcgg cgacggcggg gggaattcct ttttccgtgg tgccctttgg
      961 ggtgttggga gaagaggggg agcgccgggg atggcttgga gaatgttcct gtcgggaacc
     1021 ccagggggtc tgggggggcg acggtgtggt ggggttggtc ttttccaccc ccagccggga
     1081 ggatccggta ccgctttgct gggcccgaag cgcccgcagg gcggggcttc cggtggtctt
     1141 tctgctggac aattggatga actatcggcg acgcctggaa atggacgggc tccctgtgct
     1201 gattcccgac cggtatgcgg tgatggatgc cctggcccgg gaagaggcgg tggcggacgg
     1261 aatccctccg gaggtgatcc gggtgacggg gcaccccgcg ctggcccttt tggcccggga
     1321 gagggcccgg ggaggagggg gggaacgggg gctcgtggtc tttctctccg aaccggtgtc
     1381 ccaggaccag ggggaaggtc ccgaaaaccc ctcgtttcgg ggatacaccc aagagggggt
     1441 gttccgtcgc ctgggggcgc tgctgcgtcg cacggcggag ccctggaccc tggccgttca
     1501 accccatccc cgggaggatc cccagcgctg gcacgatctg ttccggagcg tctggaccgg
     1561 cgaggggggg gtggggctct tttccggagg gcgggaagcg gtgtgggcgg cccggggtgt
     1621 ggcgggcatg gcgtctctct tgcttcacga ggcctggctc ttgggaaaac cggtgctgag
     1681 cctccagccg gggcttcgaa atccgcaatt tcgaggggtt ttgcgacgca aggaaatctt
     1741 cggcgtgacc gataccaaag agggaagtgc ggcggaaaga gggtggcttc gggcgctgga
     1801 aaagggaaac cctgttcccc gtcccgagct ggaagaacac gccggggccc ccgatcgggt
     1861 gctggccatc atgagagagt tgacggaggg gatcgcatga gcacattggc ccttttggga
     1921 ggtactccgg tacggacgga aaagttcccc ggctatcggg tgatgggaca ggaggaaaag
     1981 gacgccacgg cccgggtcat cgattcgggg gtgctctcgg ggttcatcgc ggggtggcac
     2041 gaaaacttct gggggggccc cgaggtgcgg gccctggaag aggaatgggc ggcccacttt
     2101 cgggtaaagc acgccatcgc ggtgaattcc gccacgtcgg ggctcatggc ggcggtggga
     2161 gccgtggggg tggaacccgg agacgaggtc atcgtgcctc cgtacaccat gagcgccacg
     2221 gcggtggcgc ctctggtgtg gaacgcggtg ccggtttttg ccgatctgga gccccggggg
     2281 ttctgtctgg accccgtttc ggtggaggcc cgcatcactc cccggaccag ggccctgatg
     2341 gcggtggata ttttcggcca tccctacgac gcggacgcca tcaacgctct ggcccgcaag
     2401 tatggtctgg cggtcatcga ggactgtgcc caggctccgg gggccacgta ccggggaagg
     2461 ttcgccggaa cgctgggcaa tctgggggtt ttttctctca actttcacaa acacatccac
     2521 tgcggcgagg gagg
//
LOCUS       DAOOER010000150         2530 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_39663_length_2530_cov_7.611717, whole genome shotgun sequence.
ACCESSION   DAOOER010000150 DAOOER010000000
VERSION     DAOOER010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39663_length_2530_cov_7.611717"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PL162_09955"
     CDS             <1..482
                     /locus_tag="PL162_09955"
                     /inference="COORDINATES: protein motif:HMM:NF016177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92383.1"
                     /translation="PVAKDFTDLQLALFEAADEYPGVPIVLTGTGGGRFDHAFSALFS
                     ALWAHRRGGRVVALGDSKELLIPLRGPTEVALAFAPPPDVVSSLALSSECSGVTLKGT
                     EWPLEGVTLYLEEPYAVSNRLTMGVNDRTFEVIQGWLGVYAAWDIDLWAPSIDDRRG"
     gene            complement(497..691)
                     /gene="rpmB"
                     /locus_tag="PL162_09960"
     CDS             complement(497..691)
                     /gene="rpmB"
                     /locus_tag="PL162_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HQF92384.1"
                     /translation="MSRVCACCGRGPTTGNRVSHSVRHTRRRWLINLQKVKVDLGCGE
                     SLRLPICTKCLRSGTVRRAV"
     gene            991..1284
                     /gene="xseB"
                     /locus_tag="PL162_09965"
     CDS             991..1284
                     /gene="xseB"
                     /locus_tag="PL162_09965"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HQF92385.1"
                     /translation="MSFSEDIQQLELIIRRLEEERMPLEEALSLFRRGTEVFRGCRSF
                     LREASQEIRLLSENDPDDVTGSPWAPESAGSPGRPDPSSPASETSKEEKSERI"
     gene            1271..2173
                     /locus_tag="PL162_09970"
     CDS             1271..2173
                     /locus_tag="PL162_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HQF92386.1"
                     /translation="MSEFDAVRNTLAARQKTVDSALEGLCGPCPPGVPDRLWQSMRHS
                     LLAGGKRLRPVLCMAGSELAGSTPDRGLPMGIAFEMVHTASLIHDDLPCMDNDVLRRG
                     LPTNHVIFGESLALLAGDALLAWAFSYPSSLLVQAGVSAERIVRATEIFAQAVGPWGI
                     CGGQVLDSDPLSIREGDDFLWDIARGKTATLIQGALLSGAILGGAGPEIEERTAEYGL
                     HLGLAFQIVDDILDATAPVEELGKTPGKDAAQGKRTFVTHFGLDRSRDFAQRESDAAR
                     RALEGIPRGEWLRSLASYLVHRCS"
     gene            2187..>2530
                     /locus_tag="PL162_09975"
     CDS             2187..>2530
                     /locus_tag="PL162_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HQF92387.1"
                     /translation="MALLDTVNSPEDLQRLAPGALPWLCAELRKKIIETTLKNGGHLA
                     SSLGAVELIVALLRTFNFREDRIVFDVGHQTYAYKLLTGRKDRFDTLRRQGGLSGYPK
                     RDESPFDHFDVG"
BASE COUNT          516 a          753 c          762 g          499 t
ORIGIN      
        1 accccgtggc caaggacttt accgacctcc aattggccct ttttgaggcg gcggatgaat
       61 atccgggagt tcccattgtg ctaaccggca cagggggagg gcgttttgac cacgccttca
      121 gcgccctgtt ttccgctctt tgggcccatc gtcgcggagg aagagtggtg gcattgggag
      181 acagcaaaga gctccttatt cccctccggg gtcccaccga agtggccctc gccttcgctc
      241 ctccgccgga cgtggtgtcc tcgctggctt tgtcttctga gtgctctggg gtcaccctaa
      301 aggggaccga atggcccctt gagggagtga cgctatacct ggaagaaccc tacgccgtca
      361 gcaatcgtct gacgatgggg gttaatgata ggaccttcga ggtgatccag gggtggctgg
      421 gggtatacgc cgcgtgggac atcgaccttt gggcaccgtc gatcgacgac agaaggggat
      481 aaaaaaacgg gctgtcttag acagcccgcc gcacggttcc gctacgaagg cacttggtgc
      541 agatgggcaa acgcagcgac tcgccacatc ccaggtcgac tttgaccttc tgaagattga
      601 tcaaccaacg acgccgggtg tgacgaaccg agtggctcac cctgtttccc gtcgtcggcc
      661 cacgaccgca acacgcacat accctggaca tgactcacgc ctccttcaag actgtcggag
      721 ggcgccggag atcgccgcac cccccgttcc cggtgctcca caaggcaaat cgggggctac
      781 tataccataa tcctggggaa aacgtaaaga acccctgagg gaaaagccct ttgacacatt
      841 tattttgccg gagacggcaa ggaacgaagc aagtagcatt aaaacacatc gatgtggttc
      901 atgcccaaca cctgatcccg cctatcttcg gccttctcag ggggcggtcg gaggaagcaa
      961 taaggagcta atgaagaagg aggagtgggc gtgtcttttt cggaagatat ccagcaatta
     1021 gaattaataa ttcggcgttt agaagaagaa cgcatgcccc tggaagaagc cctttcactg
     1081 ttccgccggg ggacggaagt gtttcggggc tgtcgatctt ttcttcggga ggcatcccag
     1141 gaaatccgcc ttctgtcgga aaatgacccc gacgacgtga cggggtcccc ctgggctccc
     1201 gaatcggcgg ggtcccccgg acgtcccgac ccctcttctc cggcgtcgga aacctcaaag
     1261 gaggaaaaaa gtgagcgaat ttgatgccgt tcgaaatacc ttggccgccc gacagaagac
     1321 ggtggacagc gccctggagg gcctctgtgg cccttgtccc cccggtgtac ccgatcgcct
     1381 ctggcaatcc atgcgccact cgctgctcgc agggggtaaa cgccttcggc cggttctttg
     1441 catggcagga agcgagctgg cgggcagcac ccccgatcgg ggcctgccga tggggatcgc
     1501 ctttgaaatg gtacacaccg cctctttaat ccacgatgac ttaccctgca tggacaatga
     1561 cgtccttcga cgggggcttc ccaccaacca tgttatcttc ggcgaatccc tcgccctgct
     1621 tgcgggggac gccctcctgg cctgggcttt ctcttatccc tcctctctac tggtccaagc
     1681 tggggtatcc gccgagagga tcgtgcgcgc cacggaaatt ttcgcccagg cggtggggcc
     1741 ttggggcatc tgcgggggcc aggtgctgga ctccgacccc ttgagcatcc gcgaaggcga
     1801 cgacttcctg tgggacatcg cccgggggaa aaccgccacc ctcatccagg gagctttgct
     1861 atcgggggcc attttgggag gagcgggacc ggagattgag gaacgcacgg cggagtatgg
     1921 ccttcacctg gggctggcct tccaaattgt ggacgacatc ctcgacgcca cggcccccgt
     1981 agaagagctg ggaaaaactc ccggtaagga cgccgcccag ggcaagagga cctttgtcac
     2041 tcactttggc ctagatagat ctcgggactt cgcccaacgg gaatccgacg ccgcccggcg
     2101 ggccttggag ggaatccccc ggggagagtg gctgcgatct ttggcgtcct atttggtcca
     2161 tcgatgctct tgaggaggat gatggcatgg cgcttctaga cacggtaaac agcccggaag
     2221 acttacaacg cctcgctcca ggggcattgc cctggctctg cgcagagctt cggaaaaaga
     2281 tcatcgaaac gacgctgaaa aacggcgggc accttgcctc ttccctgggg gccgtggagc
     2341 tcatcgtggc gctgcttcgc accttcaatt ttcgagagga ccgcattgtc ttcgacgtgg
     2401 ggcaccaaac ctacgcctac aagctgctga ccggcagaaa ggatcgcttt gacaccctga
     2461 ggcggcaagg agggctcagc gggtacccta aaagggacga gagccccttt gatcacttcg
     2521 acgtggggca
//
LOCUS       DAOOER010000151         2521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_39855_length_2521_cov_4.083536, whole genome shotgun sequence.
ACCESSION   DAOOER010000151 DAOOER010000000
VERSION     DAOOER010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39855_length_2521_cov_4.083536"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            168..380
                     /locus_tag="PL162_09980"
     CDS             168..380
                     /locus_tag="PL162_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92388.1"
                     /translation="MSNVMELIKKIEGLAADPHAKPGALDGALNEMVTLLTKDSQAWD
                     LCTDTVRFQLNERFSYTGPMTYGGYR"
     gene            823..1467
                     /locus_tag="PL162_09985"
     CDS             823..1467
                     /locus_tag="PL162_09985"
                     /inference="COORDINATES: protein motif:HMM:NF037679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQF92389.1"
                     /translation="MAEPGARKEGLREGPPEVSRPERGGKSFRDAILDAAEDLIVERG
                     AAGASLDKVAARAGVSKGGLLHHFPSKNALAQALVQRVVESFDEVRAEERGEGPLDAE
                     LILRTHISWWNQVDSKRRRLYTSLLVATAHDPALVAPLALECQKELRNYEEAGIPPGR
                     VSVIMMALRGLWMTELLGITLGDYNQRLFMDELFRLAKTSSPEGEKERAVDSHA"
     gene            1460..>2521
                     /locus_tag="PL162_09990"
     CDS             1460..>2521
                     /locus_tag="PL162_09990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQF92390.1"
                     /translation="MPKRVLVLATLMLLALGGYGVARFVGPANSREVKGASVDQPQAM
                     PVVTVPAEVRSFEDSLKVQGTLRAKNFALVSPRSDGMLEEIAVNEGDRVEAGKTLLFR
                     GDTTNLEKRVQMARQDLLAARFAREQQAASVRKTDVELRRAKSDFDRFEALYREQAVS
                     KSVYEDKQAVYDQLQSMKQLVQSGLAMARETERKAEASLAVAEKDLQDAYMYAPISGV
                     VTKRMAEPGEMGSRGKPILRIDDVGTVEAVAYLPGQFYGDVREGRTSVRLGEEERWER
                     VAPVTYKSPVVDPTLRTFEIRTVLSGDGEHVVPGVLVNMRVVRTERTGVGVPYEALLE
                     RNSGKVIFLTDGHTAREVPV"
BASE COUNT          475 a          698 c          925 g          423 t
ORIGIN      
        1 agatggatcc cccggggacg tcggcggggg cccgctacgc ctcccgggac ggaggaataa
       61 tcaccaccgc cttccgcgtc tgcggggggc tgctctatgt tcttttgtct cgaaatattc
      121 cccggtaggg gggagcgggg cctctttaag aaggagggct taaagtcgtg tctaacgtca
      181 tggaattaat aaaaaagatc gagggcctgg ccgccgaccc ccatgccaag cccggagccc
      241 tggatggggc cctcaacgag atggtgaccc ttctgaccaa ggattcccaa gcctgggatc
      301 tgtgcaccga cacggtgcga ttccagttaa acgagcgatt tagctacacg gggcccatga
      361 cgtacggcgg ctatcgctag aagaccaggg ccgtcgtcgt cgctttttcc ggaagctaaa
      421 aaaattaagc ccccagggat cccgaaaggg agcggcgagg agctgccccc cttcgggggg
      481 gtgggggtgg atttaggggt cgacgcctcg tcctggcgct tttttgcgaa gggcggggcg
      541 tcgacgacgt tttgcgcgcc cccgcgcctc ccacaggggc tggggcacct caggtgaccc
      601 atagagggag gattgacaga cccggggcgg gggagtaaca taccgtccag acggtctgtt
      661 ttttccgaga gaggagagag gggcatggca gagagggact catgggagct tcgccccgaa
      721 ggccccgggg gaaatgggag cgagacatcc tcgggccttc ctcggcccag ggtgagcgga
      781 aaggcctccc ggagggcgcc tcgcggcgag ggagaaggcc ttgtggcgga gcccggcgcg
      841 aggaaagagg gtttgcgtga gggccctccg gaagtttccc ggccggaaag gggaggaaag
      901 agcttcagag atgcgatctt ggatgcggcg gaggacctca tcgtggagcg cggtgcggcg
      961 ggggcgagcc tggacaaggt ggcggcccgg gcgggggtga gcaagggagg ccttttacac
     1021 cactttccct ccaaaaacgc cctggcccag gcccttgtcc agagggtggt ggagagtttt
     1081 gacgaggtgc gggccgagga gcggggcgag ggccccctgg atgcggaact gatcctccgg
     1141 acccacatct cctggtggaa ccaggtggat tccaaacgga gacgccttta cacctctctc
     1201 ctcgtggcca cggcccacga ccccgccctg gtggctcccc tggccctgga gtgccagaag
     1261 gagttgcgaa actacgaaga ggcggggatc ccccccggcc gggtgtcggt gatcatgatg
     1321 gcgctccgcg gcctgtggat gacggagctc ctggggatca ccctggggga ttacaaccag
     1381 agattattta tggacgagct tttccgattg gcgaaaacat cctccccgga gggagaaaaa
     1441 gaaagggcgg tggattccca tgcctaagag ggtattggtg ctggcgacgc tgatgctttt
     1501 ggcgctgggg ggttacgggg tggctcgctt tgtggggccc gctaactctc gggaagtcaa
     1561 gggggcgtcg gtggaccagc cccaggcgat gcccgtggtg acggtccccg ccgaggtgcg
     1621 atccttcgag gattcgttga aggtccaggg gaccctccgg gccaagaact tcgccttggt
     1681 ctctccccgg tcggacggca tgctggaaga gattgccgtg aacgaaggag atcgggtgga
     1741 ggcggggaag acgctccttt tccgagggga cacgacgaac ttggaaaagc gggtgcagat
     1801 ggcccgccag gaccttttgg ccgcccgttt tgcccgggaa cagcaggccg catcggtgcg
     1861 caagaccgac gtggagcttc gtcgggcgaa aagcgacttc gatcgcttcg aggccctgta
     1921 ccgggaacag gcggtgagca agagcgttta cgaggataaa caggccgtct acgaccaact
     1981 ccagtccatg aagcagttgg tgcaaagcgg cctcgccatg gcccgagaga cggagcgcaa
     2041 ggcggaggcc tctctggccg tggcggaaaa ggacctccag gacgcttaca tgtacgcccc
     2101 catcagcggc gtggtaacta agcgcatggc ggagcccggg gagatgggca gccgaggaaa
     2161 gcccatcctg cgcatcgacg atgtggggac tgtggaagcg gtggcctacc ttccgggaca
     2221 gttctacggc gacgtgcggg aggggcggac ctccgtgcgg ctgggagaag aggagcgctg
     2281 ggagcgcgtc gcccccgtga cgtacaagag ccccgtggtg gaccccaccc tgcggacctt
     2341 cgagatccgc acggtgcttt cgggggatgg ggagcacgtg gtgccggggg tgctggtgaa
     2401 catgcgggtg gtgcgcacgg agcgcacggg ggtgggggtt ccctacgagg ccctgttgga
     2461 gcgcaactcg ggaaaggtga tttttcttac cgacgggcac accgcccggg aggtgccggt
     2521 g
//
LOCUS       DAOOER010000152         2513 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_40035_length_2513_cov_6.303499, whole genome shotgun sequence.
ACCESSION   DAOOER010000152 DAOOER010000000
VERSION     DAOOER010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40035_length_2513_cov_6.303499"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PL162_09995"
     CDS             <1..308
                     /locus_tag="PL162_09995"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQF92391.1"
                     /translation="VAVTGVSWGILAATGEWGPLGRASWRCLGGIVVAGAFSLTVGNL
                     FWSYALHYVTVGVATCINATKPFVTATFAAIFLGEKVTPRLAWGMVLVVGGVLLLGR"
     gene            complement(377..2365)
                     /locus_tag="PL162_10000"
     CDS             complement(377..2365)
                     /locus_tag="PL162_10000"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HQF92392.1"
                     /translation="MTHPFEASGTGRNVLAVDFGTATTYLCKCPGDQLSPQGVLLGGE
                     KDGVATALLYRQGREPLVGDEALAEFGEATERERRGYELRAQFKPDIARGRDAARYAG
                     DFLAGLLREARRQRLDVEPEGREVFFGIPSEANAEFRSALVRIAREAGYGDIQCVDEP
                     LGALAYHVFHRDLPARDALRGLLVVDFGGGTCDFSFLRRGRVGASWGDMTLGGRLFDD
                     LIYSWLLDQNPGLEDRLRASGDEFFVQTHLCREIKEAFSRSMARDRSKPFSRGVRHYG
                     RLNAMTWEDFLLRARTFRPSEAFQRFQHSLGADPEALSSEPADLVGRFRRTLTEGFLR
                     GRISPGEIRFVVLTGGSSQWPFVADVLKEELGVGEDRIMRSDRPNAAIAEGLALFPAL
                     RRAHREARRTLEEDFPRFCAQELEPLGRRYAVAAAREEAEIVTRELFDGPILQRLRLF
                     QERGGSLGQLRRELEEAAAAFSPRHREIGVRLGQGLLEELPKELGGRTEDFFRHHGFQ
                     MAEDEEALPSPVEGAILVAPDPLDPLSRPAETLVLALFAGAGAVVSGGGGMALIASGP
                     LGLAIGAGLGLVAGWTLLTQGREWSRHKLEDLSLPPAVTRSLWSGKALKALRDKFLED
                     LTQNAETNLSSLAQELVHRETLRYEGEMGALSELQKLF"
BASE COUNT          414 a          841 c          838 g          420 t
ORIGIN      
        1 cggtggcggt gacgggggtt tcctggggca tcttggcggc gacgggagag tggggccccc
       61 tggggcgggc gagctggagg tgtctgggag gcattgtggt ggccggggcg ttcagcctca
      121 ccgtggggaa cctcttttgg agctacgcgc tccactacgt gacggtgggg gtggccactt
      181 gtatcaacgc caccaagccc ttcgtcacgg cgaccttcgc ggcgatcttc ctgggagaaa
      241 aggttacgcc ccgcctggcg tggggcatgg tgctggtggt gggtggggtg ttgctcctcg
      301 ggcgatgagc cccgagacga cccggcccgc cagggccttg atggtcctcc cggggcttac
      361 cgctggcgat cgaacctcag aagagctttt ggagttccga aagggctccc atctccccct
      421 cgtatcggag cgtttcccgg tgtaccagtt cctgggccag ggacgaaagg ttggtttcgg
      481 cgttttgcgt aaggtcttcc aggaatttat cgcggagggc ctttagggcc ttcccgctcc
      541 agaggcttcg cgtcacggcc gggggaaggg aaaggtcttc aagcttatgg cgagaccact
      601 cccgcccctg ggtcagcagg gtccacccgg cgactagccc aagccccgcc cctatcgcca
      661 gccccagagg gccgctggcg atcagggcca ttcctccccc tccgctcacc acggctcccg
      721 ccccggcaaa gagggccaat accagggttt ccgcaggccg tgacaagggg tccaggggat
      781 ccggggcgac caggatggcc ccttcgacgg gggagggcag ggcctcctcg tcctccgcca
      841 tctggaatcc gtggtggcga aaaaaatctt cggtacgccc ccccaactcc ttggggagct
      901 cttcgagaag cccttgtccc aggcggactc cgatctcccg gtgccggggg gaaaaggcgg
      961 cggcggcctc ctccagctct cggcggagct gtcccaagga gccccccctt tcctgaaaga
     1021 ggcggagcct ctggaggatg gggccgtcga agagttcccg ggtcacgatc tccgcctcct
     1081 cgcgggcggc ggcgacggcg tagcggcgtc ccaaaggttc cagctcttgg gcacagaatc
     1141 gagggaagtc ttcctccaag gtccgtcggg cctcgcgatg ggcccgccgg agggctggga
     1201 agagggccaa cccttcggcg atggcggcat tggggcgatc gctgcgcatg atccgatcct
     1261 ccccgacccc cagttcctcc ttcaggacgt cggccacgaa gggccactgg ctgctccctc
     1321 cggtgaggac cacgaaacgg atctccccgg gggatattcg cccccggaga aatccctccg
     1381 tcaaggtacg gcggaaacgc cccaccagat ctgcgggctc cgaagagagg gcctcggggt
     1441 ccgcccccag ggagtgctgg aatcgttgga aggcctcgga gggacgaaag gttcgggccc
     1501 ggaggaggaa atcctcccag gtcatggcgt tcaggcggcc gtagtggcgg acccctcggg
     1561 agaagggctt ggagcgatcc cgggccatgg aacgggagaa ggcctccttg atctcccgac
     1621 acagatgggt ctggacgaag aactcgtccc ccgaggcacg cagccgatcc tccaggccgg
     1681 ggttctgatc caggagccag gagtagatca ggtcgtcaaa gagcctaccc cccagggtca
     1741 tgtctcccca ggacgctccg acccgccccc gacgcaagaa ggaaaaatcg caggtgcccc
     1801 cgccgaaatc caccactaaa agcccccgaa gggcgtcccg ggcggggaga tctcggtgga
     1861 atacgtggta ggccaaggcc cccaggggtt catccacgca ttggatatcc ccataccccg
     1921 cctcccgggc tatgcggacc agggcggagc gaaactcggc gttggcttcg ctggggatgc
     1981 cgaagaagac ctctcgcccc tcgggctcca cgtccaagcg ctgccgccgg gcctctcgca
     2041 ggagccccgc caggaaatct ccggcgtacc gcgccgcatc gcgtccccgg gcgatgtcgg
     2101 gcttgaactg ggcccggagc tcgtatcccc ggcgctctct ttccgtggct tcaccgaatt
     2161 cggccagggc ctcgtctccc accaagggct ctcgtccctg gcggtagagc agggcggtgg
     2221 ccaccccgtc cttttccccc cccagcagga ctccttgggg ggagagctga tctccggggc
     2281 atttacacag ataggtggtg gcggtgccaa agtccaccgc cagcacgttc cgtcccgttc
     2341 cagacgcctc gaaggggtgg gtcatgaggc gctccgatcc cttcgcgcca gcccccggcg
     2401 cttcaagacc ccctcaaaga acaccggggc ccgctcgatg cggagggggt ctccgggagc
     2461 cacttggccg aaggtctcat agtgatcctc catcccctct tcccagtggg ttg
//
LOCUS       DAOOER010000153         2481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_40748_length_2481_cov_7.751443, whole genome shotgun sequence.
ACCESSION   DAOOER010000153 DAOOER010000000
VERSION     DAOOER010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40748_length_2481_cov_7.751443"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..1810)
                     /gene="ricT"
                     /locus_tag="PL162_10005"
     CDS             complement(110..1810)
                     /gene="ricT"
                     /locus_tag="PL162_10005"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HQF92393.1"
                     /translation="MLSLEEPVELPKGGDVLVRTSRGEERAVCCGLLSPDALGRLGQT
                     FFGCPPEEAEESGGRGGAFLKPLSASGSRSFHGKEEPRGAHRNGGENDVEEVVYLGPL
                     EEEHRQSLAALRLEETDALREARVMLREHKLDMKLVDVEYPLDRKKIFLYFTSEQRVD
                     FRGYVKDLAKIFKTRIELRQIGIRDEAKVVGGIAPCGLPCCCSHWLHRFAPICIRMVK
                     EQNLALNPVKISGLCGRLMCCMGYEYTTYHELWERLPNPGSKIRGPRGVYAVSGVDLA
                     TQSVRLYGEGKEISVAVNDFPAFKKSLAEGTVWEPEASSPTTSDRRQDGKSRLEQDSA
                     TSLSPRRSGTEGKRSLDNLGKDRPSRQGEAPGGPRKKDGKGESPASALTDGTPGELNG
                     DRPLRGDRGGKKGRPSGGDGEKSLSRARPQDQKPSLLNQGDPLLALLPPENARDEATW
                     RGAEDPDLPLAPEKKRHRKRRRPSSKPDLPGERPEEPSVRPEKNSSEEAQKGEGSRPR
                     KGESIQGVPRDETADLRKPDESPEGGKTSSKRKRHRPRHRKSSPNSLEKFPTTPSEGS
                     "
     gene            complement(1827..>2481)
                     /locus_tag="PL162_10010"
     CDS             complement(1827..>2481)
                     /locus_tag="PL162_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HQF92394.1"
                     /translation="LFLCPGGSGSDGCPTCGAWRGEDHPDLVEAGAPGVPPGIGECRE
                     MTGGLYLAPVAGKRKVAVIYAADRLSLPAANSLLKGAEEPPTTALVWLLLESDSLLPT
                     LRSRACSLDLRPRNEKTDYGGSPPPFPQDRDPGAWGTWLLSPEWGRDRSADRFRQDLQ
                     TWSLCLSEQGALRQAEVLEIIRALGEGQRLSLPMMQDLICLALKEDYPIENVLNALW"
BASE COUNT          448 a          737 c          729 g          567 t
ORIGIN      
        1 gtggtccaga gagccatggg catctcctcc ttagggctat cttttcatcg aaaaatgcga
       61 ctcggggctg cggggtaaag ttttccccac agaattccat cccgggttct caagacccct
      121 ccgatggggt tgtggggaat ttctccaaag aattaggaga ggatttcctg tgtcgaggac
      181 ggtgacgctt gcgcttagag gaggttttcc ccccctccgg agactcgtct ggcttccgaa
      241 ggtccgccgt ttcatcccga ggtacccctt ggatagattc ccctttgcgc ggacgcgatc
      301 cttcgccctt ttgcgcctcc tcggaagagt ttttctcggg gcgaaccgaa ggctcctcgg
      361 ggcgttcccc gggtaagtcg ggctttgacg aggggcgccg gcgctttcta tggcgctttt
      421 tctccggggc cagaggaagg tcaggatcct ctgcccctct ccacgtcgcc tcatccctgg
      481 cattttccgg cggcaacagc gccagaaggg gatcaccttg gtttaggagg gaaggctttt
      541 ggtcctgggg ccgggccctg gagagactct tttccccgtc gccccccgaa ggtcttccct
      601 tctttccccc tcggtctcct cgcagagggc gatctccgtt aagttctccc ggtgtcccat
      661 cggtaagggc tgaggcggga gattcacctt ttccgtcttt ttttctgggt cccccggggg
      721 cttccccctg gcgagagggg cggtctttcc cgagattatc gagggaccgc ttcccttccg
      781 tcccggatct tcggggggat agggacgtgg cggagtcttg ttccaagcgc gattttccgt
      841 cctgtcggcg atccgaagtg gtgggagagg aagcctcggg ctcccagacc gtgccctcgg
      901 ccaagctttt cttgaacgcg gggaaatcgt tcacggcgac ggagatctct ttgccctcac
      961 catagaggcg tacgctctgg gtggccagat ccactccgga taccgcatac actcctcggg
     1021 ggccacgaat ttttgacccc ggattgggga ggcgttccca aagctcgtga taagtggtgt
     1081 actcgtaacc catacaacac ataagtcttc cgcaaaggcc ggagatcttc acggggttga
     1141 gggcgaggtt ttgttctttc accatgcgga tgcagatggg ggcaaaacgg tgaagccagt
     1201 ggctacaaca gcagggcaag ccgcaggggg caatgcctcc gacgactttt gcctcatcgc
     1261 gaatgccgat ctggcgcagc tcaatgcgtg ttttgaatat tttggcgagg tctttgacgt
     1321 agcctcggaa atccacccgt tgctccgatg tgaagtacag gaaaatcttt tttcggtcca
     1381 aggggtactc cacgtcgacg agtttcatgt ccagcttgtg ttctcgtagc atcacccgcg
     1441 cttcccgcag ggcatcagtt tcctctagcc gtagcgcagc caaggattgt cggtgttctt
     1501 cctccagagg cccgaggtaa acgacctcct ccacgtcatt ttctcccccg ttccgatggg
     1561 ctcctcgggg ctcttccttg ccgtggaaag agcgggagcc cgatgcggaa aggggtttca
     1621 aaaaagcgcc ccctcggcct ccgctttctt cagcttcttc cggaggacat ccgaagaagg
     1681 tttgtccgag gcgccccaag gcgtctgggg acaggagccc gcaacagacg gcccgttctt
     1741 ctccccgaga ggtgcgaacc aagacatcgc caccctttgg tagttcaaca ggttcttcca
     1801 gggaaagcat gccaaggtat cggggtttac catagagcat taagtacgtt ttcaatggga
     1861 taatcctcct tgagagcgag gcagataaga tcctgcatca taggaagaga gaggcgttgt
     1921 ccctctccca gggctcgaat gatttccaaa acttcagcct gacgtagggc cccttgttct
     1981 gataaacaaa gactccacgt ctggagatcc tggcgaaagc gatccgcgct ccgatcgcgc
     2041 ccccattccg gggataggag ccacgtgccc catgccccgg ggtctcggtc ctgggggaat
     2101 ggcggtggtg agcccccata gtcggttttt tcgtttcggg ggcgaagatc tagagagcac
     2161 gcccgactac gcagggtggg aaggagggag tcgctctcca ggaggagcca caccagggcc
     2221 gtcgtcggag gctcttcagc cccttttagt agactgtttg ccgcagggag tgaaagtcga
     2281 tccgccgcgt agatcacggc cacttttctt ttccctgcca ccggcgctag atagagccct
     2341 cccgtcattt ctcggcactc tcctatgccg ggagggactc cgggggctcc ggcttccacc
     2401 aaatctggat ggtcttctcc tcgccatgct ccgcaggtgg ggcaaccgtc ggagccgctc
     2461 ccccccgggc acaaaaaaag g
//
LOCUS       DAOOER010000154         2470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_41010_length_2470_cov_9.375983, whole genome shotgun sequence.
ACCESSION   DAOOER010000154 DAOOER010000000
VERSION     DAOOER010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41010_length_2470_cov_9.375983"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PL162_10015"
     CDS             complement(<1..542)
                     /locus_tag="PL162_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HQF92395.1"
                     /translation="MRCDRCHEREAEVHIRESGDDGVREHHLCRECAARHALPGGIPI
                     LSLEVMMGGGGEIPPFKEVFKKIFRVSREEEGEEEGEEEAPGAISPDTPRSCLRCGAE
                     AEEILRQGKIGCAECFRTFRAPLEEVLRNHLGDGTYRGLCPSPPSGEMMENWGVVWAL
                     RRELDRAVALEAYERAAELR"
     gene            complement(646..1125)
                     /locus_tag="PL162_10020"
     CDS             complement(646..1125)
                     /locus_tag="PL162_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009163540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HQF92396.1"
                     /translation="MSSLTKLIEAYIEDLLEEQEDPDLSLRRKELAARFGCVPSQINY
                     VLSSRFTPERGYIVESQRGGHGFIRIMKVRVKSAEERLENLENLVGESLSERDSRRLL
                     AYLHEKGVISSRERLLVDVALRHQEELARQEFDVSPYRREVLQADLLKRLLRSVILA"
     gene            complement(1268..1888)
                     /gene="plsY"
                     /locus_tag="PL162_10025"
     CDS             complement(1268..1888)
                     /gene="plsY"
                     /locus_tag="PL162_10025"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HQF92397.1"
                     /translation="MTVLWILLAYLLGSFPTGLVVVRLVRGVDVRTVGSGNIGAANVS
                     RAAGRGWATLVAVTDMLKGGVGVILARLSGITDPWILSGVGFAGVCGHNFPCWLRFRG
                     GKGVATSFGVLACLEIVTFPGGSLSWLAPLLGGGVWFGTVRLTGYAAVASLLALAVQP
                     PLLLLLGAPGPHGAFAVALFLLAVGRHRENLRRLRAGSENRVGGRR"
     gene            complement(1885..>2470)
                     /locus_tag="PL162_10030"
     CDS             complement(1885..>2470)
                     /locus_tag="PL162_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HQF92398.1"
                     /translation="TFAEARSAAAELGYPVALKVMSPAIPHKTEAGVVALRLKDEEEL
                     MNAYGRLRERAERFAPGAPIQGLLVQEMVEGGVECFVGVKRDPLFGPMVAVGLGGIYV
                     EVFRDVVLRRAPVAPQEGEEMVRSLRGYPLLAGVRGQGPRDVEALGRLIGAVSDFACG
                     EEELFELDVNPVMVREEGRGVVAVDALMTKGGRS"
BASE COUNT          425 a          923 c          746 g          376 t
ORIGIN      
        1 tcccgaagct ccgccgccct ctcgtaggcc tccagggcca ccgcccggtc cagctcccgc
       61 ctgagggccc acaccacgcc ccaattttcc atcatctccc ccgatggggg cgaagggcac
      121 aggccccggt acgtcccatc gccgaggtgg ttccgcagga cctcctccag aggggcccga
      181 aaggtccgga agcactcggc gcacccgatc tttccctggc ggaggatttc ctcggcctcg
      241 gcgccgcatc ggaggcagct ccggggcgtg tcgggggaga tggccccggg ggcctcttct
      301 tcgccctctt cttcgccctc ttcttcacgg gagacgcgga aaatcttttt gaagacttcc
      361 ttgaagggcg ggatctcccc acctcctccc atcatcacct ccagggagag gatcggtatg
      421 cccccgggga gggcatggcg ggcagcgcac tcccgacaga ggtgatgctc tcgcaccccg
      481 tcgtcccccg attcccggat atggacctcc gcttcccgtt cgtgacagcg atcgcatcgc
      541 atcccatcac cctctttcgt catcgccccc gcgagggccg cagggcaaag ggcccggagg
      601 ccccagggcg gcgccccaca cccctggcgg ggtaggcttg aaaatctagg ccaagatcac
      661 gctgcgcagc aggcgcttca ggagatcggc ctggaggacc tcccgccggt agggggagac
      721 gtcgaactcc tgccgcgcga gctcttcttg gtggcgcagg gccacgtcca ccaggaggcg
      781 ttcccgggag gagatcactc ccttctcgtg caggtaggcc aaaagcctcc gggagtccct
      841 ttcggaaagg ctctccccca cgaggttttc caggttttcc aggcgctcct ccgcgctttt
      901 tacccgcacc ttcatgatgc ggatgaagcc gtgccccccg cgctggcttt ccacgatgta
      961 cccccgttcg ggggtgaatc ggctactgag gacgtagttt atctgactcg gcacacaacc
     1021 aaagcgagcc gccaactcct tgcgccgaag ggaaagatcg ggatcctcct gttcttccag
     1081 gagatcttcg atataggctt cgatgagttt ggtcaggctg gacatggtga acaccccttt
     1141 gaaaagcggg gcggatccgg gacgaccctg cgcccccgga gccgccttga cctttcctga
     1201 ttttctcgat tatagggaaa aggtcaacaa acgtcaactg cccgagcccg acccaccgcc
     1261 gccgccctca ccttcgcccc cccacccggt tctccgatcc cgcccggaga cgacggagat
     1321 tctcccggtg acgccccacc gccagaagga agagggcgac ggcaaaggcc ccgtggggcc
     1381 cgggagctcc caggaggagg aggagcgggg gctggacggc aagggccaac agggaggcca
     1441 cggcggcgta gcccgtgagc cgcaccgtgc caaaccacac cccccctccc aggagaggag
     1501 ccagccagga gaggctccct ccggggaacg tgacgatttc caggcaggcc agaacgccga
     1561 aggaggtggc cactcccttg cccccccgga agcggagcca gcaggggaag ttgtgcccgc
     1621 agaccccggc aaatcccacg ccggagagga tccagggatc ggtgatcccc gatagccggg
     1681 ccaggatcac ccccacgcct cctttgagca tgtccgtcac cgccaccagg gtggcccacc
     1741 cccgccccgc cgcccggctg acgttggcgg cgccaatgtt gccggagccc acggtgcgca
     1801 cgtccactcc ccgcaccagg cgcaccacca cgaggcccgt gggaaaagag cccagaaggt
     1861 acgccaggag gatccaaagg accgtcatga ccggcctcct ttcgtcatca aggcgtccac
     1921 cgccacgact ccccgcccct cttcccgcac catgacgggg ttcacgtcca gctcgaagag
     1981 ctcttcctcg ccgcaggcga agtcggagac ggcgccgatg aggcgcccca gggcctccac
     2041 gtcccggggc ccctggcccc ggactcccgc cagcaggggg tagccccgga ggcttcggac
     2101 catttcttcc ccctcctggg gggccaccgg ggcccggcgg agcaccacgt cccggaagac
     2161 ttcgacgtag atccccccca atcccaccgc caccatgggg ccgaaaaggg gatcgcgctt
     2221 cacgcccacg aaacactcca cgcccccctc caccatctcc tgcacgagaa gtccctggat
     2281 cggggccccg ggggcgaacc tctccgcccg ctcccgcagg cggccgtagg cgttcatgag
     2341 ctcttcttcg tccttcaggc gcagggccac cacccccgcc tcggtcttgt gggggatggc
     2401 cggggacatg accttgagcg ccacgggata ccccagctcc gccgccgcgc tccgggcctc
     2461 ggcgaaggtg
//
LOCUS       DAOOER010000155         2433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_41995_length_2433_cov_6.137090, whole genome shotgun sequence.
ACCESSION   DAOOER010000155 DAOOER010000000
VERSION     DAOOER010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41995_length_2433_cov_6.137090"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PL162_10035"
     CDS             complement(<1..401)
                     /locus_tag="PL162_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HQF92399.1"
                     /translation="MKDRYIFPAVFHYASDGISVSFPDLPGCFSCADTDEEAVQMAEE
                     ALGLHLCGMERDGDDIPEPTQGEKIPTKPNERIFLVHVWMPQAREEIEPVYVKKTLTI
                     PADLNEAGKAAGINFSQVLSSSLRDILRRKKV"
     gene            590..1036
                     /locus_tag="PL162_10040"
     CDS             590..1036
                     /locus_tag="PL162_10040"
                     /inference="COORDINATES: protein motif:HMM:NF016754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HQF92400.1"
                     /translation="MEVRVLGLREALRELRSLGEGNVLREACAETVDRCADRVLESAK
                     ALVPVETGALRDSLKKRHKKGSLVAKVFADYPDTGEYRKKKTNKQAAGSPVYYAFAVE
                     YGTKGTKTRRARRAKPFLYPAARANIAAGDRDMEEAIERVLSRGGR"
     gene            1023..1382
                     /locus_tag="PL162_10045"
     CDS             1023..1382
                     /locus_tag="PL162_10045"
                     /inference="COORDINATES: protein motif:HMM:NF022810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HQF92401.1"
                     /translation="MGAADVCRTLYERLTGHATLAAVVTGVFDEPPKEQQAPYIVVGA
                     LQQLEGRLLDGGERKGIVQLHIWSAYKGRKETLRVAALIDEALAGDVFLFDEMEDFPD
                     DESGWWHGVASYRFYFR"
     gene            1407..1817
                     /locus_tag="PL162_10050"
     CDS             1407..1817
                     /locus_tag="PL162_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92402.1"
                     /translation="MASKNSIVMVEVSGSPVALTEVRDWSLEMERGTIDASVLSTEWK
                     RFLVGQISATGSMNLFFDPTDSAVETAMDAALFNGAAITLLFRPQGSGSGKPQYKVPA
                     IITSKGVSAATEDAVGISIGFSANAAVDNAAQSA"
     gene            2089..>2433
                     /locus_tag="PL162_10055"
     CDS             2089..>2433
                     /locus_tag="PL162_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92403.1"
                     /translation="MAQAVISTEVEPLRNMGILLERTGKQAVIQSGEMRLVVGNDIES
                     IRQGLIKIMELNFGGAMAQAAGTFDGLVKTMGGLWDKFKTDLMGSAGSPGLCHAVGQT
                     PRRSRRASCKVLE"
BASE COUNT          485 a          615 c          848 g          485 t
ORIGIN      
        1 actttctttc tgcgcagaat gtcgcgcagg gaggacgaaa ggacctggga gaagttgatc
       61 cccgcagcct tcccggcctc gttcaagtct gccggaatgg tgagagtttt cttgacgtaa
      121 acaggctcaa tctcttctcg tgcctggggc atccagacgt gcacgaggaa gatcctctcg
      181 ttgggtttgg tggggatctt ttccccttgg gtcggctccg gaatatcatc gccgtcacgt
      241 tccatgccgc agaggtgcag gccgagggct tcttctgcca tctgaacggc ttcctcgtct
      301 gtatcggcac aggagaagca accgggaagg tctgggaagg aaacggaaat tccgtcgctt
      361 gcgtagtgga agactgctgg gaagatgtat cgatctttca aggcgttccc cttctttcct
      421 agggtggcgg agctatttca gtcccgcctg cttcagtatc gaattttcgg tgccttttgg
      481 caaatctttt ttgttgcttg ggattgtgac ctttcctgac gcagacctgg gatgtacggc
      541 tgaattcgtg gcctccgacc ccctaccgca tccccataga gccggtgcaa tggaagtgcg
      601 ggttcttggg ttgagggaag cgcttcgaga gctccgatct ctgggagaag ggaacgtttt
      661 gcgggaagcg tgcgccgaaa ccgtagaccg ctgcgccgat cgggtgctgg aatcggcgaa
      721 agccctggtt ccggtggaaa ccggagcact gagggattcg ctgaagaagc gacacaaaaa
      781 gggctctctc gtggccaagg tgttcgcgga ctatcccgac accggggaat atcgaaagaa
      841 gaaaacgaac aaacaagcgg ctggatctcc agtctactac gcctttgcgg tggaatacgg
      901 caccaagggg acgaaaacga gacgggcaag gcgggcaaaa ccgtttttgt acccagcagc
      961 gagggcgaat atcgcagccg gggacaggga catggaagaa gccatagaaa gggtgttgtc
     1021 ccgtgggggc cgctgatgtc tgccggacgt tgtacgaacg cctgacgggg catgcgacgc
     1081 tcgctgcggt ggtgaccggc gtgtttgacg agccgccgaa agagcagcaa gctccgtaca
     1141 tcgtggtggg tgccctccag cagcttgaag ggcgtttgct cgacggagga gaacggaagg
     1201 gcatcgtgca gctgcacatc tggagcgcct acaagggcag aaaagagacg cttcgggtgg
     1261 cggcgctcat tgacgaggcc ctggcgggag acgtgttcct cttcgatgag atggaggatt
     1321 tcccggatga cgagtcggga tggtggcacg gggtggcgtc ctatcggttt tatttcagat
     1381 agaggagagg ggggatagtc gtggcagtgg cgtctaaaaa ttcgattgtt atggtggagg
     1441 tctcggggtc gccggtggcg ctcaccgagg tgcgtgactg gtccctggag atggaacggg
     1501 ggaccatcga tgcgtcggtg ctgtcgacgg agtggaaacg gtttttggtg gggcagatct
     1561 cggcgacggg gagcatgaac ctcttcttcg acccaacaga cagcgcggtg gagacggcca
     1621 tggatgccgc gctcttcaac ggcgcggcga tcacgctcct gttccggccc cagggctcgg
     1681 ggtcggggaa gccacagtac aaggttccgg cgataatcac gtcaaagggc gtgtccgcag
     1741 ctacggagga cgccgtcggc atttccatag ggttctcggc gaatgccgcc gtggacaacg
     1801 cggcacagtc tgcctagggg ggagcggcat gcgagaaatt gatctcggcg gtaccgtcat
     1861 ggaggtgcat tacggcatca atgccataac ggacatcgag aaggagctgg atatctccat
     1921 cgttgacctg ttgcagcgga gcggagatgt ggggccctcg atccgcgagg ttgttgtggt
     1981 gctgtgggcg gggttgcggg cgaagcgccg gggggtgacg ctggaacaaa cgggcaaatg
     2041 gctggacgac gttccgaaca tcctccccgt ggcgcaggcg tgcgggacgt ggcccaggcc
     2101 gtcatctcca ccgaggtgga acccctgcgg aacatgggca ttctcctgga acggacgggg
     2161 aaacaggccg tcatccagtc cggggagatg cgtctcgtgg tggggaacga catcgagagc
     2221 atccggcaag ggcttatcaa gatcatggag ttgaacttcg gtggcgccat ggcccaggcg
     2281 gcggggacgt tcgatggttt ggtgaagacc atgggggggc tctgggataa attcaagacc
     2341 gacctcatgg ggagcgccgg cagcccgggg ctctgtcatg ctgtgggtca gaccccgagg
     2401 cgttcccgca gggcctcctg caaggtgctg gaa
//
LOCUS       DAOOER010000156         2397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_42907_length_2397_cov_7.401793, whole genome shotgun sequence.
ACCESSION   DAOOER010000156 DAOOER010000000
VERSION     DAOOER010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42907_length_2397_cov_7.401793"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..1388)
                     /locus_tag="PL162_10060"
     CDS             complement(126..1388)
                     /locus_tag="PL162_10060"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HQF92404.1"
                     /translation="MRNMKDMKELFEMALGLGTEWHVTDITLSPEEHRIDISLDFDRG
                     TKFPCPKCDTPGCGVHDTLERTWRHLNFFEFETYLHARMPRVSCDRCGVHQVTVPWAR
                     PGSGFTLFFESLAMALMRMMPVEAAAKILNVWSTRLWRLLHRQIDGELASLDLSDVRY
                     VGVDETSSRKGHNYITVFANLEQNRVIHVAEGRGIDTLESFRSHLEIHGGHADQMELF
                     SCDMSTTFTAGILAHFPGAEIVYDRFHVVKLVQKALDQVRREERLRDPKLAQTKYIWL
                     RNPENLSATQKVRLEELTRPAHHLQTARAWAIKESFRDIFRENDLACAHACFRHWYFW
                     ATHSRLAPMIRVAKGLKNHQDRILRSVETGVSNGILEGLNSLIQAAKAKARGFRTLKN
                     LKAACYLVGGKFELGISHLWWMTHTRCY"
     gene            1666..2037
                     /locus_tag="PL162_10065"
     CDS             1666..2037
                     /locus_tag="PL162_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92405.1"
                     /translation="MTQELRETSQENDDVERRFDDELWKEIIGKFFHPMLRSALPKLA
                     KDADVSKPVVFMETELRRLARFTRRYSQSPPDSRKFVDILAVVPLLSGADKWVLLHVE
                     IQGRGGREERYLSTDLSLFPG"
BASE COUNT          489 a          722 c          678 g          508 t
ORIGIN      
        1 gtacagtagc tataagtcga cggttttttt gtcttgacaa tgggggccac cataggggcc
       61 gtgcctggag atcatccatg gcagggagat ctgcggcgaa tgtagtaggg gcgaaaaatc
      121 ttggttcaat agcacctagt gtgggtcatc caccagaggt ggctgatccc gagttcaaat
      181 ttaccgccaa caaggtaaca agccgccttg agatttttaa gtgtgcgaaa tccgcgagcc
      241 ttcgctttgg ccgcctggat gaggctgttc aacccctcca ggatgccatt gctcactccg
      301 gtctccacgg agcgaagaat gcggtcctgg tggtttttca agcccttggc cactcgtatc
      361 atgggcgcaa gacggctgtg cgtggcccag aagtaccagt ggcggaaaca ggcatgggca
      421 cacgcaagat cgttctcgcg aaagatgtcc cgaaaggatt cttttatcgc ccaggcccga
      481 gccgtttgca ggtggtgtgc ggggcgcgtc aactcttcca gccgaacttt ttgtgtcgca
      541 ctcaggtttt cagggttccg gagccagatg tacttggtct gcgccagttt cggatcccga
      601 agccgctcct cgcggcgcac ctgatcaagg gccttttgca cgagctttac cacatggaag
      661 cggtcataaa cgatctccgc ccccggaaag tgtgccagga tgcccgctgt gaaggtggtg
      721 ctcatatcgc aggagaaaag ttccatctgg tccgcatgcc ctccgtggat ttcaagatgg
      781 ctccggaaag attcgagggt gtcgatgccc cttccttcgg ccacgtggat gacccggttc
      841 tgttcaaggt tggcgaagac ggtgatgtag ttatgcccct tgcgggagga tgtttcgtca
      901 acaccgacgt accgcacatc ggagaggtcg agggacgcaa gttccccatc aatctgccgg
      961 tgaagcaacc gccacagcct ggtgctccag acattcagga ttttggccgc cgcctccacc
     1021 ggcatcatgc gcatcagggc catggccaac gattcgaaga acagggtgaa accgctccct
     1081 gggcgcgccc aaggcaccgt cacctggtgg acaccgcaac ggtcgcagga gacccggggc
     1141 atccgggcgt ggagataggt ttcgaactcg aagaagttca gatggcgcca ggtgcgctcc
     1201 agggtgtcgt gaaccccgca cccgggggtg tcgcacttgg ggcaggggaa tttcgtgccc
     1261 cggtcgaagt cgagggagat gtcgatccga tgctcctcag gcgagagcgt gatgtcggtg
     1321 acgtgccact cggttcccaa ccccaacgcc atttcaaaga gctccttcat atccttcatg
     1381 tttctcatgc ctccatcgcc gcctcgggag acggaaaagt aaactcctgt gggggaagac
     1441 taccacaaca actacgaggg ctggagagga tgcccacaaa aaatgctata gaaccaaaat
     1501 ttttcgcccc taggcaaccc ctttcgcaga ccatccacag cccttcccca gacctccgga
     1561 aggatcttcc gtagtccccc cacagatcgc tctccggggc tcccccagtg cgccccgttg
     1621 tgctatcctg taaaatcaga agccaacaca aggggggaga agccagtgac ccaagaactg
     1681 cgagagactt cccaagaaaa cgacgatgtc gagcgacgct tcgacgacga gctctggaag
     1741 gagatcatcg gcaagttctt tcaccccatg cttcgctccg ccctccccaa gctggcaaaa
     1801 gacgccgatg tctccaaacc cgtggtcttc atggaaacag agctccggcg cctggcgcgc
     1861 ttcacccggc gatacagcca aagccccccg gactcccgga agtttgtcga tatcctcgcc
     1921 gtggttcccc tgctctccgg agcggacaag tgggttttgc tccacgtgga gatccagggg
     1981 cgcgggggcc gggaggagcg gtatctgtca accgacctgt cgcttttccc gggttaaagt
     2041 ctctcggact cttccgtcct tttccggatt gagccatacc actttctctt ctccaagatt
     2101 cctgatcggg cggcctttcc accgctccgg atgtctgcgc ctcacctcct caaatacctc
     2161 cttcctcgac gcgagtatcc cggcggactc cccccggtga cgctgacccg gcgtgacgta
     2221 cttcagggcg ctgtgatagt gaatctcgtt gtaccaatgt acgaagcggt gcgtccactc
     2281 tctggccgcc tggaggttct cgaagccaaa aaccggatat tcgggacggt acttgagcgt
     2341 ccgtgttccc gctccctctg tctgtccggg aacggcttaa catcggtggc cttttag
//
LOCUS       DAOOER010000157         2365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_43753_length_2365_cov_5.296104, whole genome shotgun sequence.
ACCESSION   DAOOER010000157 DAOOER010000000
VERSION     DAOOER010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43753_length_2365_cov_5.296104"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1680)
                     /locus_tag="PL162_10070"
     CDS             complement(<1..1680)
                     /locus_tag="PL162_10070"
                     /inference="COORDINATES: protein motif:HMM:NF017400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HQF92406.1"
                     /translation="MKLPRCGSSSNVPRRPFKGLLGALLGAWVLFFWNGNSGDAEASG
                     PFVPRDPVFPVSQLRSGMKGEAKTVVRGLEVTSFPVEIVDVLRREGLPRHLVLVRVSG
                     ALMDRTGGIAAGMSGSPVYVQNKLVGALGYGWEFSDHRLGLVTPAEDLTEVWNWPDRL
                     PSFRPPVPLSGDRSEEGQGEGFSGVLLAEYFSPSGGYFAFGGVHLTFGERSSFGPEAP
                     LQSPLLSDGMSPRARERLGSSLGVRVLAGGGTPSDLFIDERATLRPGDSVGVLLAWGD
                     VTVGSTGTLSALDRQGRFVAFAHPMLNRGNVAYPLARSVVHGVVPSVEVPFKLGTPQS
                     LIGIVTQDRPQGVGGYLGRFAPAVDFSVTVRDGDTKREIKKRFHVVDDPFLLEEIAPE
                     AVLGLLDDAWGRVGPGTIFSTLRVEGGGLSQGWKRENIFFSTKDPVAEVVKECRDLVK
                     AFALNEFQEIRPLGVHLEAEFSLDPRVLFVEDVILSPQEAAPGQKVQVSVTLRPYRRE
                     PFEKIFTLTVPKDAEGTCDVLVRGGGIAEPDQESLLQGWRSIKDLPSLLAEL"
     gene            complement(1677..>2365)
                     /gene="prfB"
                     /locus_tag="PL162_10075"
     CDS             complement(1677..>2365)
                     /gene="prfB"
                     /locus_tag="PL162_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_143330735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HQF92407.1"
                     /translation="ADWADMLHRMYLRWAEIEGFQTTILDLSPDIEAGIKSATVLVKG
                     AFAYGYLKAERGVHRLVRISPFDAAKRRHTSFASLGVAPEMPEDAGVEIRSEDLKMDT
                     FRASGAGGQYVNMTDSAVRITHIPSGIIVSCQVERSQHMNRATAMRILRARLFERTLR
                     ERQEKLEALGGEKKEIGWGSQIRSYVLHPYTLVKDHRTNYEVGNVQAVLGGELQDFIM
                     AYLRWKKKNQ"
BASE COUNT          476 a          750 c          689 g          450 t
ORIGIN      
        1 aagctcggcg aggagggacg gaaggtcctt gatggatcgc cacccttgta gtagcgattc
       61 ctgatccggt tcggcgatgc cccctcctcg cacgaggacg tcgcaggtcc cttcggcgtc
      121 tttaggcacg gtgagcgtga aaattttttc gaagggttcc cgccgatagg gacgaagggt
      181 gacggagacc tgcaccttct ggcccggagc cgcttcctgg ggcgatagga tcacatcctc
      241 cacgaaaagc acccgggggt ccagggagaa ctccgcttcc aagtgaaccc ctaaagggcg
      301 gatttcctga aattcgttga gggcgaaggc tttgacgaga tctcggcatt ctttgaccac
      361 ctctgccacg ggatctttcg tggagaagaa aatgttctcc cgcttccatc cttgcgaaag
      421 cccccctccc tcgacgcgaa gcgtgctgaa aatcgttcct ggccctacgc gtccccaggc
      481 gtcgtccagg aggcccaaga ccgcctcggg ggcgatctct tccagcagga aggggtcgtc
      541 cacaacgtga aacctctttt ttatttctcg cttagtgtcg ccgtcccgga ccgtcacgga
      601 aaaatccacc gccggggcga agcggcctag gtaccccccc acaccctggg ggcggtcctg
      661 ggtgacgatg ccgatgaggc tctggggagt gcctaacttg aacggcactt ccacgctggg
      721 gaccacgccg tgcaccacag accgggccaa agggtaggcc acgtttcctc ggtttagcat
      781 ggggtgggca aaggctacga agcgtccctg tcggtccagg gcggaaaggg tgcccgtaga
      841 tcccacggtg acgtctcccc aggccaagag gacccccacc gagtctccgg ggcgtagcgt
      901 tgctctctcg tcgatgaaaa ggtccgaagg ggtacctcct cccgccagga cgcggacccc
      961 cagggaagaa ccaaggcgct cccgggccct agggctcatc ccgtccgata aaagagggct
     1021 ctgtaggggc gcctctgggc caaaagagct ccgttcgcca aaggtgaggt ggacgcctcc
     1081 gaaggcaaag taccctccgc tcggggaaaa atattccgcc aagagcaccc cagaaaaacc
     1141 ctccccttgg ccctcttcgg aacgatcccc ggagagggga acgggggggc gaaacgaagg
     1201 gaggcgatcg ggccagttcc acacctctgt caggtcttcc gcaggagtca ccagccccag
     1261 gcgatgatca ctgaactccc atccgtaccc cagagctccc accagcttgt tctggacata
     1321 gaccggggat ccgctcatgc ccgccgcgat gccaccggta cgatccatca gcgctccgga
     1381 gacccggacc aacactaagt ggcgaggaag gccttctcgg cgcaacacgt ccacgatttc
     1441 cacggggaac gaggttactt ccaacccccg taccacggtc ttcgcctctc ccttcatccc
     1501 cgaccggagc tgagacacgg gaaacacggg atcccggggg acaaagggcc ccgaagcttc
     1561 tgcgtcccca gagtttccgt tccagaaaaa aagtacccac gcccccagga gagcccctaa
     1621 aagccccttg aaggggcgac gaggtacgtt tgaagacgaa ccacatctgg gcaatttcat
     1681 tgatttttct tcttccatcg taggtaagcc atgataaaat cctgcagctc tccccccaag
     1741 acggcttgga cgttacccac ctcgtagttg gtgcgatggt ctttgaccag cgtgtaggga
     1801 tggagaacgt aggaacggat ctggctcccc catccgatct cttttttctc ccctcccagg
     1861 gcttcgagct tttcctggcg ctcccgcagg gtgcgctcaa agaggcgagc cctcaggatg
     1921 cgcatggccg tggcgcgatt catgtgttga gatcgctcca cttgacagct gacgatgatc
     1981 cccgaaggga tatgagtaat ccgcacggcg gagtcggtca tgttgacgta ctgtcccccg
     2041 gcgccgctgg ctctaaaggt gtccattttc aggtcttcgc tacggatctc cacccccgcg
     2101 tcttcaggca tttccggggc cacgcccagg gatgcgaagc tcgtgtggcg tcttttggcg
     2161 gcgtcgaagg gggatatacg gaccaagcga tggactcccc gctctgcctt gaggtagccg
     2221 taggcgaagg ctcccttcac cagcacagtg gcgcttttta tccccgcttc gatgtcgggg
     2281 gaaaggtcca aaatggtggt ctgaaaccct tcgatttcgg cccaccgaag gtacatgcgg
     2341 tgcagcatgt ccgcccaatc cgccg
//
LOCUS       DAOOER010000158         2360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_43887_length_2360_cov_6.198265, whole genome shotgun sequence.
ACCESSION   DAOOER010000158 DAOOER010000000
VERSION     DAOOER010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43887_length_2360_cov_6.198265"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PL162_10080"
     CDS             <1..229
                     /locus_tag="PL162_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92408.1"
                     /translation="TLLQGERVLGTLFVVRSQAGAEALPEETLELRALRLAFRQALEA
                     YRQGIERKDPAVEPLRRKAVLAWLEYGSRRP"
     gene            complement(491..676)
                     /locus_tag="PL162_10085"
     CDS             complement(491..676)
                     /locus_tag="PL162_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92409.1"
                     /translation="MDAYRSVTRRPAPRKLIIMDACVRIDFIKAERAVLELVVKHIGP
                     PHVTSPVVGEVNEIVVL"
     gene            complement(715..1323)
                     /locus_tag="PL162_10090"
     CDS             complement(715..1323)
                     /locus_tag="PL162_10090"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HQF92410.1"
                     /translation="MRTLLGFLGALGVWGCLTFPSAGAWGGEVRGEVWKDGGQPLPGV
                     LVRMEGELCPRARTDDRGSFSLRSPSEDPVTLLVSPDTLPAVRISGLGGGPGNNVRVD
                     LGYLFARAGEPLSLDLPSNPSTGYGWSLAESGDVPFTAESAFIPPPPSGPRPTVGRGG
                     TERWTFIFPSPGFYSLRLVYARPWEQERPPARTQVVVVTVAP"
     gene            complement(1387..>2360)
                     /locus_tag="PL162_10095"
     CDS             complement(1387..>2360)
                     /locus_tag="PL162_10095"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HQF92411.1"
                     /translation="TPSDSARNRLEALLGAFFGEREFQHVLREPWGSPGYDSSDLEPR
                     HWITMTRILSEEMARGLDGAIILHGTDTMAYTSAWLSVALGSVPIPVVLTGSQLTLDY
                     TPEDVLVNLRGAAQVLCAGHPGVWIYFNWKLLPGNRAHKARATHPDAYVPVNGVPVYF
                     SPDWLKDPKGAEVPPRAPWSPSPETARLLALSDREDPLDGALGWHFCAPGCPATFSGR
                     ERILGLIGLGSGNAPQSVLRACVDRFRSDPKPLILGCSQAEGDRKNPSAYKHVGIASL
                     AEEGFSVWSQGEYPLEFVHALGAFALLGAPEDPSAVLDRYLRRSPAP"
BASE COUNT          436 a          789 c          784 g          351 t
ORIGIN      
        1 gaccctcctc cagggcgaac gggtgctggg gaccctcttc gtggtgcgct cccaggcggg
       61 agcggaggct ctccccgaag agaccctcga gctccgcgcc ctgcgtctgg cgttccgcca
      121 ggccctagag gcctaccgcc aagggataga acgcaaagac cccgccgtgg aacccctgcg
      181 gaggaaggcc gttcttgcct ggttggagta cggctcccgg cgcccttgaa tcaacctcaa
      241 ggatgcgctc cggcggcagt tcacccccgc tggagcgcat cctgggagcc ttggcacaaa
      301 gggaagaaaa tcgcgtgaaa attacgcgat cacatcgttg atcgggaaaa ggcgaaagcg
      361 aagcgggccg ccaatcctgg ggcccgaccc gccctgacga tccggcaggc gaccatacca
      421 tcaagaccgc cattctcccc gtccgcatcc ggccccaggc tcctggcgga aaggcgttcg
      481 tcaagttggc ctagagcacc actatttcgt taacctcgcc cacgaccggg ctggtcacat
      541 gcggcggacc gatatgtttc accacgagtt ccagcacggc ccgctccgcc ttgatgaaat
      601 caatcctcac gcacgcatcc atgataatca acttgcgggg agccgggcgg cgcgtcacac
      661 tccggtaggc atccaacgga gacgttctct tgagatgaac ccgggagggc cccatcaggg
      721 agcgacggtc accaccacca cttgggtcct tgcggggggc ctctcctgct cccagggacg
      781 ggcgtagacg aggcgcagcg agtaaaaccc cggggagggg aagataaagg tccaccgctc
      841 cgtgcccccc ctccccactg tggggcgggg ccccgagggc gggggaggaa tgaaggcgct
      901 ctctgcggta aaagggacat ctccggactc cgccagggac cagccatatc ccgtggaagg
      961 gttggaggga aggtccaagg acaggggctc ccccgcccgg gcaaagaggt agcccaggtc
     1021 cacccggacg ttattccccg ggcctccccc gagccctgag atgcgcacgg cgggaagggt
     1081 atcgggggac accaggaggg tgacgggatc ctccgacggg gatcggaggg aaaagctccc
     1141 ccgatcgtcg gtgcgggccc tcgggcagag ctccccttcc atccgtacca gaacccccgg
     1201 caggggctga cccccgtctt tccacacctc accccttacc tcgccccccc aagcccccgc
     1261 agaagggaag gtaagacacc cccacacccc caaggccccc aagaaaccga ggagagtcct
     1321 catgtcgtca tcccctttct catttcccat cgctccgggg gccgccgccc tcgccgggac
     1381 cagcccctag ggggctggag aacggcgcag gtagcggtcc agcaccgcag aagggtcctc
     1441 cggagccccc agaagggcga aggcccccag ggcgtggacg aattccagag ggtactctcc
     1501 ctggctccat acggaaaagc cctcctccgc caaggaggcg atgcccacgt gcttgtacgc
     1561 cgaggggttt ttcctgtccc cttcggcctg gctgcatccg aggatcagtg gtttgggatc
     1621 cgatcggaaa cgatcgacgc aggcccgcag gacgctctgg ggggcgttgc ccgatcccag
     1681 gccgatgagc ccgagaatgc gctctctccc cgagaaggtg gcggggcacc cgggggcgca
     1741 gaagtgccac cccagggccc cgtccaaggg gtcttcccgg tcggaaaggg ccaggagacg
     1801 ggccgtttcg ggggaagggc tccagggagc ccggggcggc acctccgccc ccttggggtc
     1861 tttgagccag tcggggctga agtacacggg gacgccgttt acggggacgt aggcatcggg
     1921 atgggtggcc cgggccttgt gggcccggtt ccccgggagg agcttccaat tgaagtaaat
     1981 ccacaccccg gggtgtccgg cgcataggac ctgggccgcc ccccgaaggt tcaccaggac
     2041 gtcttcgggg gtgtaatcca gggtgagctg gctgccggtg agcaccacgg ggatgggaac
     2101 actccccagg gccacgctga gccacgccga ggtataggcc atggtgtccg tgccgtggag
     2161 gatgatggcg ccatcgaggc ctcgggccat ttcttccgaa aggatgcgag tcatggtaat
     2221 ccagtgacgg ggttccaggt cggagctgtc gtaaccgggg ctcccccagg gctcccggag
     2281 gacgtgctga aactccctct cgccgaagaa ggcgccgagg agcgcctcca ggcggttccg
     2341 ggcgctatcc gaaggggtgg
//
LOCUS       DAOOER010000159         2345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_44300_length_2345_cov_5.780786, whole genome shotgun sequence.
ACCESSION   DAOOER010000159 DAOOER010000000
VERSION     DAOOER010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44300_length_2345_cov_5.780786"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..724
                     /locus_tag="PL162_10100"
     CDS             <1..724
                     /locus_tag="PL162_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92412.1"
                     /translation="GAMGFLGEFDQFLALTPEIEEVAHLLVHCREEARRLKDKRDRLA
                     EKYKGKSDAERRLELQDNFKRKRMFVEMGARRSRLHPSALYADSFTPPITFSEISRHL
                     ATFSSLDPGLFSSRRFRVHGYPRIGIMPGRGNGVYDWEDHALLFPLFPASTPERSVAQ
                     ALGLLRWDADDDRDLKDTYGALKDNRGKSIVGLQEDFCKEYLVWLTKEAKGYRVLPKE
                     SYNWFHWKIAGGSELGADVGKK"
     gene            735..1613
                     /locus_tag="PL162_10105"
     CDS             735..1613
                     /locus_tag="PL162_10105"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQF92413.1"
                     /translation="MQLFFALITLGALGALSCNAPGEGAELGAPRQAPVGDPFLVTLS
                     SDLPMTDLVLRWGGRSCSLDVQPRRGGWIARALLGTEAHTTKAGEATLEIQGREGQAS
                     ALWKHRIKILPRKFGVTRLSLDEAYVTPPKELTDRIARERRLTAAAKARYTLPQRWTL
                     PLARPVPGKITGTYGKGYILNGKPRSPHRGLDFRAPEGTEVRALAGGVVALVGEHYYA
                     GKSVYLDHGGGVISFCMHLSEIAVREGDEVKRGQVVGKSGSTGRSTGPHLHLGLCLQG
                     RLVDPSPLFERGKRAK"
     gene            1643..2341
                     /locus_tag="PL162_10110"
     CDS             1643..2341
                     /locus_tag="PL162_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92414.1"
                     /translation="MNLSDREHVLVGRAWGSVLGGVVLFLLFPPTQEIFRQLTAAHPY
                     ILGVLKIGVLGAMGDMLAVRMRGGRWRYQHIGLRDKALVWGFLGLIFVVVFPIFSVGT
                     DFLFRQGYLTNPFEGFAAKTLEAFAASSFMNLLFAPTMMVFHRVTDMAIAEGGVFRRW
                     SAVEMLRKMDWNSMGRIVFPSLLWFWIPAHTLTFILPPEFRIVSAALLAIALGFILSF
                     ASRSSGTPGRKEGR"
BASE COUNT          388 a          727 c          789 g          441 t
ORIGIN      
        1 gggggccatg ggcttcttgg gagagttcga ccaatttctg gccctgacgc cggagatcga
       61 ggaggtcgcc caccttctgg tccactgccg agaggaggcc cgccgcctca aggacaaacg
      121 ggatcgcctg gcggaaaagt acaagggcaa aagcgacgcc gaacgccgcc tggagctcca
      181 ggacaacttc aaacgaaagc gcatgttcgt ggagatggga gcccggcgga gccgcctgca
      241 cccctcggct ctctacgccg atagcttcac gccccccatc accttttccg agatctccag
      301 gcatttggcc accttttcct ccctggaccc ggggcttttt tccagccgac ggtttcgggt
      361 gcacggctac ccccgcatcg gcatcatgcc cggccggggc aacggagttt acgattggga
      421 ggaccatgcc ctgctctttc cccttttccc cgcctccacg ccggagcgat cggtggccca
      481 ggccctgggg cttttgcggt gggatgcgga cgacgaccgg gatctgaaag acacctacgg
      541 ggccctcaag gacaaccggg gcaagtccat cgtgggcctc caggaggact tctgcaagga
      601 gtacctggtc tggcttacca aggaagccaa gggatatcgg gtgctcccca aggagagcta
      661 caactggttc cactggaaga tcgccggggg atcggagctg ggcgccgatg tggggaaaaa
      721 gtagactcct ttgggtgcag cttttctttg ccctcatcac cctgggggcc ctgggggcgt
      781 tgagctgtaa cgccccgggg gagggcgccg agctaggggc cccccgtcag gctccggtgg
      841 gggatccctt tctggtgacc ctgtcgtccg atctgccgat gacggatctg gtcctgcggt
      901 ggggggggcg gagctgctcc ctggacgtcc agccccggcg aggagggtgg atcgcccggg
      961 ccctcctggg gacagaggcc cacaccacca aggccgggga ggcgaccttg gagatccaag
     1021 gaagggaagg ccaggcctcg gccctttgga agcatcggat caagattctc ccccggaagt
     1081 tcggcgtcac ccggctttcc ctggacgaag cctacgtcac cccccccaag gagttgaccg
     1141 atcgcatcgc ccgggagcgg cgccttacgg ccgccgccaa ggcccgatac accttgcccc
     1201 agcggtggac gctgcctttg gcgcgccccg tcccgggaaa gatcacggga acctacggca
     1261 agggatacat cctgaacggc aaaccccgga gcccccaccg ggggctggat ttccgggccc
     1321 ccgagggcac ggaggtgcgg gccctcgccg gaggggtggt ggcgctggtg ggggagcact
     1381 actacgcggg aaagagcgtc tatctggacc acggcggggg cgtgatctct ttttgcatgc
     1441 acctgtcgga gatcgccgtg cgggaaggag acgaggtcaa aagaggacag gtggtgggaa
     1501 aatcgggctc cacggggcgc tccacggggc cccacctgca cctggggctc tgcctccagg
     1561 ggaggttggt ggacccctcg cccctctttg agcggggtaa aagggcgaaa taacgcgatc
     1621 gaggtcgaga ggaggcgcga aattgaatct gagcgatagg gaacacgtgc tggtgggtag
     1681 ggcttggggt agcgtgctgg ggggcgtggt gctctttttg ctcttccccc ccacccagga
     1741 gatctttcgt cagctcacgg cggcccaccc ctacatcctg ggggtgctga agatcggcgt
     1801 cctgggcgcc atgggggaca tgctggcggt gcgcatgagg ggaggacgat ggcgctacca
     1861 gcacatcggc cttcgggaca aggcgctggt ctggggattt ctcggcctta tcttcgtggt
     1921 ggtctttccc atcttctccg tggggacgga cttcctcttc cgccaggggt acctgaccaa
     1981 cccctttgag ggcttcgccg ccaagactct ggaggccttc gcagcgtcgt ctttcatgaa
     2041 tttgctcttt gcgcccacca tgatggtctt tcaccgggtc accgacatgg ccatcgccga
     2101 ggggggggtc ttccggcggt ggagcgccgt ggagatgctc cggaagatgg attggaactc
     2161 catggggcgc atcgttttcc catctcttct ctggttctgg atccccgccc acactctgac
     2221 tttcatcctg ccgccggagt ttcgcatcgt ctcggcggcg cttttggcca tcgccctggg
     2281 cttcattctt tccttcgctt cccggagctc cgggaccccg gggcgtaaag aggggcgatg
     2341 atgtg
//
LOCUS       DAOOER010000160         2305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_45479_length_2305_cov_11.037778, whole genome shotgun
            sequence.
ACCESSION   DAOOER010000160 DAOOER010000000
VERSION     DAOOER010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45479_length_2305_cov_11.037778"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..871
                     /gene="nth"
                     /locus_tag="PL162_10115"
     CDS             122..871
                     /gene="nth"
                     /locus_tag="PL162_10115"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HQF92415.1"
                     /translation="MTPSRWKGDPRSSLVEVLDTLERCWHQGDLFRPDFSKEPLDGLV
                     LTVLSQNTNDRNRDMAYGRLRSAFPSWEAVAAAPREEVEEALRPAGLAPGKTKAILAI
                     LAQLHRTFGAFSLESLRLMPPEEARSFLEQLPGVGPKTAACTLLFDLAVPAFPADTHV
                     SRVCRRLGLVPPSATPPQIMAFVESFLPPSYFLPGHLNMIEHGRHRCRARSPRCDECE
                     IRDWCEFPASTSAEKEVRRPARSSNKRKDLA"
     gene            1042..>2305
                     /locus_tag="PL162_10120"
     CDS             1042..>2305
                     /locus_tag="PL162_10120"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HQF92416.1"
                     /translation="MKNLDQLELLLSFLGDGGEGASDAALGEQKNGSETRDDLRRKSA
                     QRREFNSEKPGSFPLFRVGIARCEDYAPESVQKALQQAVDRAGGWPPLPSSVLLKANL
                     LAPRAPEDAATTHPEILRGVVLSLRRDQPTRKIVVADSPGFLYTHQREALFERTGLRA
                     LAESVGCGLEILSDDGFTSVYPPGGMALLQAHVAEKFLRAPAVINVAKMKTHVETRIS
                     ACIKNIFGIADTPTRKRAHSTRDPEHLIQAIVDLYSVRPPAFAVLDAVEAMEGNGPSH
                     GTPKFLGWILAGENALAVDLVASHMMGYPDPRAVPLLREAMDRGLGPTDRGQIALCGG
                     TWEEIVCEGFQKATTRIDWVPSALKSILYQITALRPALDTARCTSCGICAQVCPVGAI
                     SLRHGRPEIDRRRCVSCMCCHEMCPPGAM"
BASE COUNT          455 a          741 c          696 g          413 t
ORIGIN      
        1 tcagcggcaa gggggataag cagaagaaag aggacgaaga agccccccaa cccccagaag
       61 ggcgcgaaaa ttcaggcgac gaggacgacg aaaacgagga cgatgaggag cgatcctctc
      121 gatgacccct tctaggtgga aaggggatcc ccggtcttcg ctcgtggagg tcctcgacac
      181 cctggagcgg tgctggcacc aaggcgatct ctttcggccg gacttctcta aagagccgct
      241 agatggcctt gtgctgaccg tcctatccca gaacacgaac gaccgtaacc gggacatggc
      301 ctatggccgg ttacggtcgg ccttcccttc ctgggaggcg gtggccgccg cccccaggga
      361 agaggtggag gaggcccttc gccctgcggg gctggccccg ggaaagacca aggccatctt
      421 agccatcttg gcccaactac accggacctt cggtgcgttc tcgctggaat ctctacgtct
      481 gatgcccccc gaagaagcgc ggagcttctt ggagcagctc ccaggggtgg ggcccaaaac
      541 cgccgcatgc accctgctct ttgatctggc cgtgcccgcc tttcccgccg acacccatgt
      601 cagccgggta tgccggaggc ttggcctcgt tcccccctcg gccacgcctc ctcagatcat
      661 ggccttcgtg gaatcctttc tgcccccctc ttacttcctc cccggccatc tgaatatgat
      721 tgagcacggc cggcaccggt gtcgggcccg gtctccccgg tgcgatgagt gcgaaatcag
      781 ggactggtgc gaatttccgg cctcgacctc cgccgaaaaa gaggtacgac gaccggcgcg
      841 atcctccaac aagcgtaagg atttggccta attctggcgc cttctccctc cggggaaaaa
      901 gcttttttgg ggggccgcct gggggcgccg tctccagggg cccatctttg ggaaggggaa
      961 aggcgtcaac ggtttctgat gccccccgta cgaagccagg ccacatcctt acgctgacgc
     1021 aaccgaaggg gaggggagac catgaaaaat ctcgaccaac tcgaactcct cctatccttt
     1081 ctcggcgatg ggggagaggg agcctctgac gcagcattgg gggagcaaaa aaacggcagc
     1141 gaaacgcgcg acgatctcag gagaaaaagc gcgcaacgac gagaatttaa ctcggagaag
     1201 ccgggatctt ttcctctctt tcgcgtgggc attgcccggt gcgaagacta cgctccggag
     1261 tccgttcaaa aagcccttca gcaagcggta gatcgggcgg ggggatggcc tcctctccct
     1321 tcttccgtgt tgctcaaggc caaccttttg gctccccgag ccccagaaga tgccgccact
     1381 acccatccgg aaattcttcg cggagtagta ctaagccttc gccgagacca accgacgagg
     1441 aaaatcgtgg tggccgactc ccccggtttt ttatataccc accagcgaga agcccttttc
     1501 gaacgcaccg gcctgcgggc cctggcggaa agcgtggggt gcggactgga gatcctctcg
     1561 gacgacggtt tcacttcggt gtatcccccc ggaggcatgg ctctgctcca agcccacgtg
     1621 gccgagaagt tcctccgggc ccccgccgtg atcaacgtgg ccaagatgaa aactcatgta
     1681 gaaacccgga tttccgcctg cataaagaac atctttggca tcgccgacac ccccactcgg
     1741 aagcgagccc actccacccg ggatccggag cacctgatcc aagccatagt ggatctttac
     1801 agcgtccgcc ccccggcctt cgcggtgctc gacgccgtgg aggccatgga aggaaatgga
     1861 ccaagccacg ggacgcccaa atttctagga tggatcttgg cgggagaaaa tgccctggcg
     1921 gtggacctgg tggcgtctca catgatggga taccccgatc ctcgggccgt gcctctgctg
     1981 cgagaggcca tggatagggg tctggggccc accgacaggg ggcagatcgc cctgtgcggc
     2041 gggacgtggg aagagatcgt ctgtgagggc tttcagaaag ccacgacgcg gatcgactgg
     2101 gtcccctccg ccctgaaaag catactgtac cagatcacgg cccttcgccc cgccctcgac
     2161 acggcccggt gcacctcctg cggcatctgc gcccaggtgt gccccgtggg ggcgatctcc
     2221 ctacgccacg gtcggccgga aatcgatcgg cggcgctgcg tctcgtgcat gtgctgccac
     2281 gaaatgtgcc ctccgggggc catga
//
LOCUS       DAOOER010000161         2291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_45863_length_2291_cov_5.907424, whole genome shotgun sequence.
ACCESSION   DAOOER010000161 DAOOER010000000
VERSION     DAOOER010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45863_length_2291_cov_5.907424"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1012
                     /locus_tag="PL162_10125"
     CDS             <1..1012
                     /locus_tag="PL162_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92417.1"
                     /translation="GLTWLSLSPDQGAVPAGGNVPVQVIFRADVPEASDPGTYHAQVR
                     VKYPSPYGTLTVPVTMTVSGAGTPTPVPTNTPVPQPTNTPGPQPTNTPGPQPTNTPGP
                     TGVPSPTSAPTPTVAPTDMPEPPVVPPEPTPVVQEGTPTGTPLPIASPDELPNTGGGT
                     QENVFISPDVVRQILGDQVSADAYQLVEVKNGEEKTFTTTTGYVTLTVTSEVSESGHA
                     VYRWIMVIWNNVTELWETVTGEIVTAGAALPAFETTTTTLQSRIADGGPYDLDGAVNG
                     IVQSRTAMALLGAPKAAPAPTTAPTGVPAGTGGGGCAVQAWGNFLPFALLLLPLVLLG
                     KK"
     gene            complement(1150..1530)
                     /locus_tag="PL162_10130"
     CDS             complement(1150..1530)
                     /locus_tag="PL162_10130"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HQF92418.1"
                     /translation="MNLIAMCGLNCGECDAYRATQAEDQEAKERVAARWREEYHSPEI
                     TADHVTCDGCVTGDRHGGYCAFCAVRACGLGRGVPNCAHCESYDGCPTLTEFYSHFPD
                     GVEACPPKVCLDGVRRTLGMIQGS"
     gene            1841..>2291
                     /locus_tag="PL162_10135"
     CDS             1841..>2291
                     /locus_tag="PL162_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92419.1"
                     /translation="MIRESSPYPFKVETPSSTPEEDPGPGGVRRDVPTLEPPFDPTGQ
                     GPLSDPLAMDPLQAPPERHVLALDSGRSALSVGLRALRRAGCAPVIHLPAVICRAVMT
                     IALHEGFSLRFYRLGRDLATPTFPSPPRAEEVVLYVHYFGWRHDAMER"
BASE COUNT          407 a          835 c          657 g          392 t
ORIGIN      
        1 gggcctgacg tggctctccc tctcccccga ccagggggca gtccctgcgg ggggcaacgt
       61 gccggtgcag gtgatcttcc gggccgacgt gcccgaggcc agcgatccgg gaacctacca
      121 cgcccaggtg cgggtgaagt atccaagccc ctacggcacc ctcaccgtgc cggtgaccat
      181 gaccgtgtcg ggagctggca cccccacgcc ggtgcccaca aacacaccgg tgcctcagcc
      241 cacgaacaca ccgggacctc aacccacgaa cacaccggga cctcaaccca cgaacacacc
      301 ggggcccacg ggagttcctt ctcccacaag cgcgccgacg cccacggtgg cccccacaga
      361 catgccggag cccccggtgg ttcccccgga gcccacgccg gtggtgcagg aaggcacccc
      421 cacgggaacc cccctaccca tcgcctcccc cgatgaactt cccaataccg gtggaggaac
      481 ccaggaaaac gtgttcatct ctccggacgt ggtgcgccag atcctgggag accaggtgag
      541 cgccgacgcc tatcagctgg tggaggtaaa aaacggcgaa gagaagacct ttaccaccac
      601 cacggggtac gtgaccctca cggtcacttc cgaggtctcc gagagcggcc atgccgtgta
      661 tcggtggatc atggtgatct ggaacaatgt cacggagctt tgggaaaccg tcaccgggga
      721 aattgtcacc gccggagcgg cccttcccgc ctttgaaacc accaccacca cccttcagag
      781 ccgcatcgcc gacggggggc cctacgacct ggatggcgcg gtaaacggca tcgtgcaaag
      841 ccgcactgcc atggccctgc tgggggctcc caaggccgcc cctgctccca cgacggcccc
      901 caccggcgtc cccgcaggca cggggggagg gggatgcgcc gtccaggcct ggggaaactt
      961 cctgcccttc gctctgctcc tgctgccctt ggtgctgttg gggaaaaagt agcttcttga
     1021 aaaaggtctt gtccaagagg tatccccgat gggggcctct cgggcaggat cccggccatg
     1081 gtcgaggccc cccggagaca cggggggcct cgatttgttt ctaaaaaagc tggttcccga
     1141 gaggccgcct cagctcccct gtatcatgcc aagcgtgcgc cgcaccccat ccagacatac
     1201 tttgggggga caggcctcta ccccatcggg aaagtgggag taaaactccg tcagggtggg
     1261 gcacccgtcg tagctttcgc aatgggcaca gttgggcact ccgcgcccca ggccgcaggc
     1321 ccggacggcg caaaaggcgc agtaaccccc gtggcgatcc cccgtgacgc acccatcaca
     1381 ggtgacgtgg tcggcagtga tctcggggga gtgatactcc tcccgccacc gggccgcgac
     1441 gcgttccttg gcctcctgat cctcggcctg ggtggcccga taggcgtcac actctccgca
     1501 gttcagtcca cacatggcga taagattcat ctcttctcct cctcgttatt ccacagtttt
     1561 tcgtccccac ctcgcctcga ctctccgaaa aagggcggat acaccccccg ccagggagct
     1621 atccatcgcc ttcggaaatc cgtcggagcc cctccggttc ctggtctcgg tcatcccacc
     1681 ccgccgggcc gtcgccccat cggccgtctc cttcggaggg cgtcaacaaa ggcctttttt
     1741 cgcccctcga ttcctttacg ccgcccccct tcccctttct ggacaccccc tttattatag
     1801 cgcaaaaggc acctaaacca tcgcccgtcg ggggataaaa atgatccgag aaagctcgcc
     1861 ataccccttc aaggtcgaaa ccccttcctc gaccccggaa gaagatccgg gacccggagg
     1921 ggttcgtcgc gatgttccca ctctagagcc ccctttcgac cccacaggcc aggggcccct
     1981 ttccgatccc ctggcgatgg atcctctcca ggcgccgccg gagcgccacg tcctcgcctt
     2041 agactcggga cgaagcgccc tttccgtggg gctccgggcc ctgaggaggg cggggtgcgc
     2101 cccggtgatt catctgcctg cggtgatctg tcgtgccgtg atgaccatcg ccctgcacga
     2161 gggcttctcc cttcggtttt accgcctggg aagggatctc gccactccca ccttcccctc
     2221 ccctccccgc gccgaggagg tggtgctcta cgtgcactac ttcgggtggc gtcacgacgc
     2281 catggaaagg g
//
LOCUS       DAOOER010000162         2279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_46226_length_2279_cov_6.453687, whole genome shotgun sequence.
ACCESSION   DAOOER010000162 DAOOER010000000
VERSION     DAOOER010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46226_length_2279_cov_6.453687"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PL162_10140"
     CDS             complement(<1..292)
                     /locus_tag="PL162_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HQF92420.1"
                     /translation="MRALNLPNLLSLSRVFLAPLVMVFLTLKITAPVKPLGSFGVDAT
                     YGDLLAGVVFILAALTDTADGYLARKHGMVTNLGKLIDPLADKILVTAALISL"
     gene            complement(508..1284)
                     /gene="trpA"
                     /locus_tag="PL162_10145"
     CDS             complement(508..1284)
                     /gene="trpA"
                     /locus_tag="PL162_10145"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HQF92421.1"
                     /translation="MAFTQAFQNKALLTYICAGDPDLGATADVLCALDDAGADVLEVG
                     VPFSDPVADGPVIQEASTRALARKTSLGGVLEMLHSVKGALRAPRVIMSYVNPLAAYG
                     FDRFARDAREGGVSGVLVPDLPRGESAPLERALSEKGVILVRMVSPNSSDRRLAAVGE
                     GAEGFIYCVSLLGVTGEGARIRDTVPAYLGRVRRHTKVPLALGFGIDGPERARALAPH
                     ADGLVVGSALVSRIHDHRGNRGALLREVKAFAASMKEVLR"
     gene            complement(1300..>2279)
                     /gene="trpB"
                     /locus_tag="PL162_10150"
     CDS             complement(1300..>2279)
                     /gene="trpB"
                     /locus_tag="PL162_10150"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HQF92422.1"
                     /translation="EDLNHTGAHKINNALGQVLLASRMGKRRIVAETGAGQHGVAVAT
                     AAARFGIACTVYMGEEDVRRQAPNVERMALLGAEVVPVRAGSRTLKDAVNEAMRDWVA
                     NVETSFFALGSVTGPHPYPTMVRDFQRVIGDEARAQILDHLGRLPDAVVACVGGGSNA
                     LGIFSAFVADEGVRLVGVEAAGEGLGTGRHAATLTRGKPGYLHGAFSYVLQDDDGQIL
                     EAHSLSAGLDYPGVGPEHSFFRDSGRVTYATVDDDEAVAAFHELSRLEGIIPALESAH
                     AVAFVRRLAPELAPGSVVLVNLSGRGDKDMYSVAAFRGGPGLRRGADLS"
BASE COUNT          403 a          810 c          730 g          336 t
ORIGIN      
        1 ccagggaaat gagcgccgcc gtgacgagga tcttgtccgc caaggggtcg atgagcttgc
       61 ccagattggt caccatgccg tgcttgcgag ccaggtagcc atcggcggtg tcggtgaggg
      121 ccgccaggat gaataccacc cccgccagga ggtcgccata ggtggcgtcc actccgaaag
      181 accccagggg cttcaccggg gcggtgatct tcagggtcaa aaagaccatc accaggggtg
      241 cgagaaagac ccgggacaga ctgagtaagt tgggcaggtt caacgcccgc atgaaccgtt
      301 cccccctctt cgcctttttt ctccacggcc aaggcatcgc cgtcgcgcca ccggggaagg
      361 ccctccgctt tccccgtagc ctaggctacc acaaaagggc gtcgatgaca ccatggggaa
      421 ggggagaacc ccacgaaaaa ggcgtccctg gtcccttgga actcccgaag gggaagaact
      481 tggctccccg cgccagaggc tccggggcta ccggaggacc tctttcatgg acgcggcaaa
      541 ggctttcacc tctcgaagca gggcgcctcg gttcccccgg tgatcgtgga tgcgggaaac
      601 gagggcactc cccaccacga ggccgtcggc atggggcgcc agggcccggg cccgctccgg
      661 accgtcgatg ccgaaaccca gggccagggg gaccttagtg tggcgccgca cccgccccag
      721 ataggcgggg acggtgtcgc ggatgcgggc cccctccccc gtgacgccca ggagggagac
      781 gcagtaaata aacccctcgg ccccctcacc cacggcggca aggcggcggt cggagctgtt
      841 gggcgacacc atgcgcacca atatgacccc tttttcggaa agggctctct ccaggggagc
      901 gctttcgccc cggggcaggt cgggcaccag gacaccgctc accccccctt cccgggcatc
      961 ccgggcgaag cgatcgaagc cataggccgc caaagggttg acgtagctca tgatcacccg
     1021 gggggctcgc agggcgcctt ttaccgagtg cagcatctcc aggacgcccc ccagggacgt
     1081 cttccgggcc agggcccggg tgctcgcctc ttggatcacg gggccgtcgg ccacggggtc
     1141 ggaaaagggc acccccacct cgaggacgtc cgctccggcg tcatccaggg cgcaaagcac
     1201 gtcggcggtg gctcccagat cgggatcccc ggcacagata taggtgagaa gcgctttgtt
     1261 ttggaaagcc tgggtaaagg ccatgacgat tcctccttgc taggataggt cggcgccccg
     1321 gcggaggccc gggcctcctc ggaaggcggc cacggagtac atgtccttgt ctccccttcc
     1381 cgagaggttc actaagacca cgctccccgg cgccaattcc ggggccaggc gccgcacaaa
     1441 ggccacggcg tgggcacttt cgagggccgg gatgatgccc tccaggcggg acagctcgtg
     1501 gaacgccgcc acggcctcgt cgtcgtccac cgtggcgtag gtcacccgcc ccgagtcccg
     1561 aaaaaagctg tgctcggggc ccacgccggg gtaatcgagc cccgccgaga gggaatgggc
     1621 ctccaggatc tgcccgtcgt catcctgcag gacgtaggaa aaggccccgt gcagataccc
     1681 gggcttgccc ctggtgaggg tcgccgcatg gcgccccgtg ccgaggccct cccccgccgc
     1741 ctcgaccccc accaggcgga ccccttcatc ggcaacgaag gccgaaaaaa tccccagggc
     1801 gttgctcccg ccccccacgc acgccaccac cgcgtccgga aggcgcccca gatgatcgag
     1861 gatctgggcc ctcgcctcat cgccgatgac acgctgaaaa tcccgcacca tcgtggggta
     1921 ggggtggggc cccgtgacgg agcccagggc aaaaaacgag gtctccacgt tcgccaccca
     1981 gtcccgcatg gcctcgttca ccgcgtcctt cagggtgcga ctccccgcgc gcacggggac
     2041 cacttcggcc cccaggagtg ccatgcgctc cacgttgggg gcctggcggc gcacatcctc
     2101 ctcccccatg tagaccgtgc aggcgatgcc gaaacgagcc gccgccgtgg ccacggccac
     2161 accgtgctgc cccgcccctg tctcggccac gatacggcgt ttgcccatcc tcgaagcgag
     2221 gaggacctgc cccagggcgt tattgatctt gtgggccccc gtgtggttca gatcctccc
//
LOCUS       DAOOER010000163         2274 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_46387_length_2274_cov_4.105904, whole genome shotgun sequence.
ACCESSION   DAOOER010000163 DAOOER010000000
VERSION     DAOOER010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46387_length_2274_cov_4.105904"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PL162_10155"
     CDS             complement(<1..543)
                     /locus_tag="PL162_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92423.1"
                     /translation="MKKTMILWGVLLAIFVGTSGAEAAPASPFTLEVIQPDGSVIAVV
                     QRGDEFFSWSETPEGYAVAQNDHTGFWEYAVERQGRLVPGGEICRRNKTPMGTATRFF
                     RPPASALRRQSVFSPGEAPQGTWSPRPLAGTRKILLIRVGFVDRPLSTDEALHREALF
                     GTAFSVKKYYADQSRGKLIIE"
     gene            complement(691..1287)
                     /locus_tag="PL162_10160"
     CDS             complement(691..1287)
                     /locus_tag="PL162_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92424.1"
                     /translation="MKGFVGALGVLVGWAGLAWGAPASPFPVEVFQPDGTRMELTNRG
                     DEFCFWVETPEGYTVIQNSGSGFWEYARKKLVARELVPGGVRVDPQGSPPPGTVPHLR
                     PRRFDALGGTPERGDPVEVSQRGKKILLVPWSDGESSWWETLSGYAVAKDRASGEWEY
                     AQLHSVEILEPSGHIYRPGIPAPSDMPRHEKPSVRTPY"
     gene            complement(1493..1693)
                     /locus_tag="PL162_10165"
     CDS             complement(1493..1693)
                     /locus_tag="PL162_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92425.1"
                     /translation="MICLFPLPPERSPLFAPFVPGFRCADGEEERRAERHWREVGRGG
                     GEIPEDPATPEDRERWRERRFS"
     gene            complement(1690..>2274)
                     /locus_tag="PL162_10170"
     CDS             complement(1690..>2274)
                     /locus_tag="PL162_10170"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HQF92426.1"
                     /translation="LAEQEGRCYISSFEDRFVVAGAGTVGVEILEEEPALGTLVSPLG
                     GGGLLSGIAVASRALGPGMALWGVTARANPCWARAWDRGRMAPVEDEKESLAEGLAGT
                     GSLRLYPFLREHLAGVRSVTEDEIARAMALLHRAHHLVVEGAGAVGVAALLTGAIPLS
                     GPVAVVLTGGNVDEAAFLSALGRGDNRQLMEDLS"
BASE COUNT          393 a          796 c          685 g          400 t
ORIGIN      
        1 ttcgatgatc agtttccctc gggactgatc cgcatagtat tttttcacgg aaaatgcggt
       61 cccgaagagg gcttctcggt gcagggcctc gtcggtgctc aaaggccgat ccacgaatcc
      121 cacccggatc aggaggatct tccgggtacc ggcgagggga cggggagacc aggtgccctg
      181 gggcgcctct ccgggggaaa aaacgctctg gcgtcgcagg gcggaagcgg gggggcggaa
      241 aaagcgcgtc gccgttccca tgggcgtctt gttccggcgg caaatctctc cgccggggac
      301 taatcgcccc tgtcgttcca cggcgtactc ccaaaaaccc gtgtggtcgt tttgtgccac
      361 ggcgtacccc tcgggggtct cgctccagga gaaaaactcg tcgccccgct gcaccacggc
      421 gatgaccgac ccgtcgggct ggatcacttc caaggtaaag gggctcgccg gtgccgcctc
      481 ggcccccgaa gtgcccacaa aaatcgccag caagactccc caaagaatca tcgttttttt
      541 catattaccc ctccttttgg ccttttgcgc tccgcgcgcc cacacaccct tgccagaaag
      601 ccgcgccttt atcccccagg gtgcgaggct ccccttcccc tccgccttct ctcctttcta
      661 aggatctcgt aggcgtttcc ccccccacct ttagtagggc gttcgcaccg acggtttttc
      721 gtgccggggc atgtccgagg gggcggggat ccccggccga tagatgtgcc ccgagggctc
      781 caggatctcc acggagtgaa gttgggcgta ctcccactct cccgaagcgc ggtcttttgc
      841 caccgcgtat ccggaaaggg tttcccacca ggaagactcc ccgtcgctcc agggcaccag
      901 aaggattttt ttccctctct gggacacctc cacggggtcg cccctctcgg gcgttcctcc
      961 gagggcgtcg aagcgccggg ggcggagatg gggcacggtc cccggaggag ggctcccctg
     1021 gggatccacc ctcactcccc cggggaccag ctcccgggcg acgagtttct ttcgggcgta
     1081 ctcccaaaaa ccgcttccgc tgttctggat caccgtgtac ccttcggggg tttccaccca
     1141 gaagcagaat tcgtcccccc ggttcgtcaa ctccatgcgg gttccgtcgg gctgaaagac
     1201 ctccacagga aagggactgg cgggggcccc ccaggccagg cccgcccacc ccaccagaac
     1261 tcccagggcc cccacgaatc ccttcatcgc ccccacctct tttcgataac agttgccctt
     1321 taaggcgcca aagcggttat ataccaaaca gccttagctg taaagaaccc tcgggggtcg
     1381 cccgagggtt ctttaggctc cttggccaag ggaggaagaa agggagggaa aaatcgtgtg
     1441 tgtcggggta aggttgcggt cccttcagga gggggtaaag cccaagattt tttcaggaaa
     1501 atcggcgctc tcgccaccgc tcccggtcct ccggagtagc gggatcttcg gggatttctc
     1561 ctcccccacg ccccacctcg cgccagtggc gttcggcccg gcgctcttcc tcgccgtcgg
     1621 cgcaacgaaa ccccggcacg aagggggcaa atagggggga gcgctccgga gggaggggaa
     1681 agaggcatat catgacaaat cctccatgag ctgacgatta tctccccggc ccagggcgga
     1741 taaaaaggcg gcctcgtcca cgttgccccc ggtgagcacc accgccacgg ggcccgagag
     1801 ggggatggcc cccgtcagaa gggcggccac ccccacggcc cccgctcctt ccaccaccag
     1861 gtggtgggcc cggtgaagca gggccatggc ccgggcgatc tcgtcttccg tcacgctccg
     1921 gacccccgcg aggtgctccc gaaggaaggg atagagccgg agagaccccg tgcccgccag
     1981 tccttcggcg aggctttctt tttcgtcttc caccggtgcc atgcgccccc ggtcccaggc
     2041 ccgggcccag cagggattgg cccgggcggt gactccccag agggccatgc cggggcccag
     2101 ggcccgggag gccacagcga tccccgagag gagtcctccg ccccccagag ggctcaccaa
     2161 agtccccagg gcgggctctt cttccaggat ctccaccccc acggtgcccg cccccgccac
     2221 cacgaagcgg tcctcgaagg aggagatgta gcaccgccct tcttgctccg ccag
//
LOCUS       DAOOER010000164         2250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_47147_length_2250_cov_6.852847, whole genome shotgun sequence.
ACCESSION   DAOOER010000164 DAOOER010000000
VERSION     DAOOER010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47147_length_2250_cov_6.852847"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="PL162_10175"
     CDS             complement(<1..1229)
                     /locus_tag="PL162_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HQF92427.1"
                     /translation="MAERLRLGDLLVKAGAISDDQLSRALEEQRVTHARLGEVLVKNG
                     WVSERQLVEALADQLKKSIVTLSRFKPMPEALRLVPEQTAKRLEIVPMSVNESGQLLV
                     AISDPLNVFVLDELRMLTGMEVDVSLAVPSEIRRSLDSVYRAPAEGLDEGKIEVMEEA
                     SEEEMDTMLSTSDAAADDAPVVKLVNSMIEQSVKEETSDIHIEPQERKTRVRYRVDGS
                     LFDAFEFSRRMHPAVSSRIKIMAGMDIAEKRKPQDGRIVIKVLGRRVDFRVSSLPTVF
                     GEKIVIRVLDQSAVMVGLDKIGFEAEDKALLEDILKVPYGIILITGPTGSGKSTTLYS
                     MLEKINRPEVNIITVEDPVEYHIQGINQVQVNEKAGLTFAESLRSILRQDPDKIMLGE
                     IRDTVTAEMAIRAALTG"
     gene            complement(1367..>2250)
                     /locus_tag="PL162_10180"
     CDS             complement(1367..>2250)
                     /locus_tag="PL162_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92428.1"
                     /translation="LENDSGALTFPLFSPGTLLSMTTYYWRVEATALAGSGLPNLTGG
                     VWRFATGQALALTPLDPADGAEDVKAPTKTLLWKVMRGEEDLSSQYTYEVRWGNSAAM
                     ANSQRNLTSPACTLTNLLYGTAYYWRVVATPRAGGSAITGPVWRFSTAAAEDLVLTFE
                     NIEPGEDAKDQRPDVSLRWDCTVGSLSVGGLRFDVRLGTTPSNLATVASLLADKLFHP
                     TTLVYEKTYYWQIVAHGVNGDGVAMTWHGPIWKFTTGRQHSEDWPLDGGGGCALGALS
                     PLEGCLFLAPFLTFFSR"
BASE COUNT          405 a          702 c          728 g          415 t
ORIGIN      
        1 tgccccgtga gggccgcccg gatggccatt tcggcggtga cggtgtcccg gatctcgccg
       61 agcatgatct tgtccggatc ctggcgcaaa atggagcgca acgattcggc aaaggtgagc
      121 ccggcctttt cgttcacctg cacctggttg atgccctgga tgtggtactc cacgggatct
      181 tccacggtga tgatgttgac ttccggacgg ttgatctttt ccagcatgga gtagagggtg
      241 gtgctcttgc cgctccccgt ggggcccgtg atgagaatga tgccgtaggg gaccttgagg
      301 atgtcctcca ggagggcctt gtcctcggcc tcgaaaccga tcttgtcgag ccccaccatc
      361 accgcgctct ggtcgaggac ccggatgacg atcttctctc cgaagaccgt ggggagcgac
      421 gatacccgga aatccacccg tcgtcccagc actttgatga cgatgcggcc gtcctggggt
      481 ttgcgtttct cggcgatgtc catccccgcc atgatcttga tgcgggagga cacggcgggg
      541 tgcatgcgcc gcgagaactc gaaggcgtcg aagagcgatc cgtccacccg atagcgcacc
      601 cgggtcttgc gctcctgggg ttcgatgtgg atgtcggagg tttcctcctt gacggattgc
      661 tcgatcatgc tgttcaccag cttcaccacg ggggcgtcgt ccgccgccgc gtcgctggtg
      721 gagagcatgg tgtccatttc ttcttcggac gcctcttcca tgacttcgat cttgccctcg
      781 tccagtccct ccgcaggagc gcggtagacc gaatccagcg aacggcgtat ttccgagggc
      841 accgccaggg agacgtccac ttccattccc gtgagcatgc ggagctcgtc aaggacaaag
      901 acgttcaggg ggtccgaaat ggccaccaga agctggccgc tttcgttcac ggacatggga
      961 acgatctcca gacgcttggc ggtctgctcg ggcaccaggc gcagggcctc gggcatgggt
     1021 ttgaagcggg acagggtgac gatgcttttc ttgagctggt ccgccagagc ttccaccaac
     1081 tggcgttcgg aaacccagcc gttcttcacg agcacttctc ccagccgggc atgggtcacc
     1141 cgctgctcct ccagagcccg ggacaactgg tcgtcagata tggctcccgc ttttacgagg
     1201 aggtctccga ggcgcaaccg ttccgccata aaaaaagttc accgcctctc tgtcacataa
     1261 aaaacctgga agccttccaa agaaaaggcc cgaaacgacg ccccgccctc cgaaaaagga
     1321 aagggggccc aaggaacccg ggggcccttg ggcccggagc catgccctat cgggagaaga
     1381 acgtcaggaa gggggcgagg aagaggcacc cctccagggg agaaagggcg cccaacgcgc
     1441 acccgccgcc gccgtccagg ggccagtctt cgctgtgctg ccgccccgtg gtgaacttcc
     1501 agatggggcc gtgccacgtc atggccacac cgtccccatt caccccgtgg gcgacgatct
     1561 gccagtagta ggttttctcg taaaccaggg tggtggggtg aaagagcttg tccgccagga
     1621 gggacgccac cgtggccaga ttggaggggg tggttcccag gcgcacgtca aaacgcagtc
     1681 ctccgacgga aagggacccc accgtgcaat cccagcgcag ggaaacgtcc gggcgctgat
     1741 ctttggcgtc ctctccgggc tcgatgttct cgaaggtcag caccagatcc tcggcggcgg
     1801 cggtggagaa gcgccacacc ggaccggtga tggcgcttcc ccccgcccgg ggggtggcca
     1861 ccacccgcca gtagtaggcg gtgccgtaca ggagattggt caacgtacag gcgggggacg
     1921 tcaaatttcg ctggctgttt gccatggcgg cggaattgcc ccagcgcacc tcgtaggtgt
     1981 actgggagga gaggtcctct tctccccgca tgaccttcca gagcagggtc ttggtggggg
     2041 ccttcacgtc ttcggcgcca tccgccggat cgaggggggt gagggccagg gcctgccccg
     2101 tggcgaagcg ccagacgccc cctgtcaggt tcgggagccc cgaccccgcc agggccgtgg
     2161 cctccacccg ccagtagtag gtggtcatcg acaggagcgt tcccggagag aagaggggaa
     2221 aggtcagggc gccggaatcg ttctccaaat
//
LOCUS       DAOOER010000165         2227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_47883_length_2227_cov_9.737569, whole genome shotgun sequence.
ACCESSION   DAOOER010000165 DAOOER010000000
VERSION     DAOOER010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47883_length_2227_cov_9.737569"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..140
                     /locus_tag="PL162_10185"
     CDS             9..140
                     /locus_tag="PL162_10185"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HQF92429.1"
                     /translation="MPAVLVEMGYITEGSDAKLLASQGFQDRMAEALAQGAEAYLNP"
     gene            283..1059
                     /locus_tag="PL162_10190"
     CDS             283..1059
                     /locus_tag="PL162_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92430.1"
                     /translation="MARWRDQRRDDDMQDEEGVDEEESLRLMAPVPQESYDAPPRRPR
                     RRREPDPDYDDDDERPRKAAWPLRLLAWGSMLALFFGIGYGVATFGLKWLDAQGKISQ
                     PEVVDSPEEAQRLLDKGPETRVSIRRGDYRLHILGKEGLESRTLGVIPGVTEEDLRTC
                     LEAVLEPLQAEAGRGDPMRVLHLFRAGDMLYLDINQPFLAALEKLPSDRGILFMTGLV
                     RSVVENFPPSVQVQIMINGKIPTAKGPIDLTVPWTLAPQS"
     gene            1064..1837
                     /gene="rph"
                     /locus_tag="PL162_10195"
     CDS             1064..1837
                     /gene="rph"
                     /locus_tag="PL162_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005659243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HQF92431.1"
                     /translation="MAFLREDGRGPGELRALLIERQVNCHAEGSALISLGRTRVHCTA
                     TVEEKVPPHLRGSGTGWVTAEYAMLPRATGGRSPRETSGVRGRSAEIQRLIGRSLRAV
                     TVLPLLGERTIILDCDVLQADGGTRTAAISGAFVALTDALRGLWREEKIRVLPLRDFL
                     GAVSVGRVGGQVLLDLAYAEDRDAEVDCNVVMTRGGALVEIQASGEEETFSRDDLDRF
                     LDVAARGVALVGAEQERALGLTAEERGALHEMADRLRLP"
     gene            1806..>2227
                     /locus_tag="PL162_10200"
     CDS             1806..>2227
                     /locus_tag="PL162_10200"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HQF92432.1"
                     /translation="MRWRIVFASHNAGKAAEVRTLLPGVELLGGVAGPAVEETGSTYG
                     ANALLKARSWAWATGLPALADDSGLEVRALGGAPGLHSARVAPSDRERLRWLLGRLEG
                     VKDRRACFVASLAVAFPREGRSLLVQGRCFGTIARAPAG"
BASE COUNT          368 a          708 c          824 g          327 t
ORIGIN      
        1 gggcggggat gcccgccgtg ctggtggaaa tgggctacat cacggaaggc agcgatgcca
       61 agctcctggc ctcccaggga tttcaggatc ggatggccga ggccctggcc cagggggcgg
      121 aggcctacct gaatccgtga gaccaagggc cccgggcgaa gggtcccggg ggacctttag
      181 acgaagctcc ggggccggga gatcggcgcc ccgggagaag atgcggcgcc ccaagggccc
      241 cgtggaggag ccctttaggc caaggcacga ggaggaatga ccgtggcacg gtggcgggat
      301 caacgcaggg acgacgatat gcaagacgaa gaaggggtgg acgaagaaga atctctccgc
      361 ctcatggcgc cggtgccgca ggaatcctac gacgcccccc ctcgccgacc tcggcgaagg
      421 agggagccgg atccggacta tgatgacgac gacgaacgcc cccgaaaggc ggcgtggcct
      481 ctgcgccttt tggcctgggg ctccatgctg gcgctctttt tcggcatcgg ctacggcgtg
      541 gccaccttcg gtctgaagtg gctggacgcc caggggaaga tctcccaacc ggaagtggtg
      601 gattccccgg aggaagccca gaggctcctg gacaagggcc ccgagacccg ggtgagcatc
      661 cggcgggggg actaccgcct ccacatcctg ggcaaggaag ggctggaaag ccgcaccctg
      721 ggggtgatcc ccggcgtcac ggaagaagac cttcgcacct gcctggaggc ggtgctggaa
      781 cccctgcagg ccgaagcggg gaggggcgat cccatgaggg tgctccacct cttccgggcg
      841 ggggacatgc tctacctgga catcaaccag cccttcctgg cggccctgga aaagctcccc
      901 tcggatcgag gcatcctctt catgacggga ctagtgcgga gcgtggtgga aaacttcccc
      961 ccctcggtac aggtgcagat catgataaat ggcaaaattc ccacggccaa gggccccatc
     1021 gacctcacgg tgccctggac cctggccccc cagtcgtagc gccatggcct ttcttcgcga
     1081 agacggccgg gggccggggg agctccgagc cctactcatc gagcgccagg tgaattgcca
     1141 tgcggagggg tcggccctca tctccctggg gcgcacccgg gtgcactgca ccgccacagt
     1201 ggaagaaaag gtcccccctc acctccgggg atcgggtacg gggtgggtga cggcggagta
     1261 cgccatgctc ccccgggcca cgggggggcg ctccccccgg gagacctcgg gggtccgggg
     1321 cagaagcgcg gagatccagc gcctcatcgg ccgatccctg agggcggtga ctgtgctccc
     1381 cctcctggga gagcgcacca tcatcctcga ttgcgacgtc ctccaggccg acggaggcac
     1441 ccgcaccgcc gccatatccg gggccttcgt ggcgctgacg gacgccctcc gaggcctttg
     1501 gcgggaagag aagatccggg tcctcccctt gcgagatttt ctgggggcgg tgagcgtcgg
     1561 gcgggtgggg ggacaggtgc ttttggacct ggcctacgcc gaggaccgtg atgcggaagt
     1621 ggattgcaac gtggtgatga cccggggagg ggccctggtg gagatccagg ccagcggcga
     1681 agaagagacc ttctcccggg atgacctaga ccgattcctg gacgtcgccg cccggggcgt
     1741 ggccctggtg ggggccgaac aggaacgggc cctgggactt acggcggaag agcgaggggc
     1801 cctccatgag atggcggatc gtcttcgcct cccataacgc cggaaaggcg gcggaggtga
     1861 ggaccctgct ccccggggtg gagctcctgg gaggggtggc gggccccgcc gtggaggaga
     1921 cggggagcac ctatggggcc aatgccctgt tgaaggcccg atcctgggcc tgggccacgg
     1981 gccttccggc cctggccgac gacagcggcc tggaggtgag ggccctggga ggggccccgg
     2041 gactccactc cgcccgggtg gccccctcgg atcgagagcg cctccggtgg ctcttggggc
     2101 gcctcgaagg cgtgaaggac cggcgggcct gcttcgtggc ctctttggcg gtggccttcc
     2161 cccgggaggg gcgatccctt ttggtccagg ggcgctgttt tggcaccata gcccgggccc
     2221 cggcggg
//
LOCUS       DAOOER010000166         2206 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_48577_length_2206_cov_3.421199, whole genome shotgun sequence.
ACCESSION   DAOOER010000166 DAOOER010000000
VERSION     DAOOER010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48577_length_2206_cov_3.421199"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="PL162_10205"
     CDS             <1..926
                     /locus_tag="PL162_10205"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HQF92433.1"
                     /translation="AVSLALLGMVLQGRWITLLQGWRAEQVMKDYGPHADRESKMGIP
                     SVGGAVFLLLGLGGWGWGLCQIPGASEEHLVVWGLALGASLVGFLDDFLKISRKSSEG
                     LSSRQKFLWQMAVCLPWAFHVVALRGWGGSVASEALLVMGVTLGANGMLNAVNVTDGL
                     DGLAAGSCALSFVALAGMVPTGSPAWWGALMGGGIATGFLWHNAHPARVFMGDGGAHF
                     LGGLLLGVAAFGRSPMAVVAVSPLFGVEILSVAIQIVALRCFHRRVFRMSPLHHHFEL
                     GGWKETQIVTRFMVLHAMGIVVLGSLGGCFN"
     gene            923..>2206
                     /gene="murD"
                     /locus_tag="PL162_10210"
     CDS             923..>2206
                     /gene="murD"
                     /locus_tag="PL162_10210"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HQF92434.1"
                     /translation="MRGSMGVSHVTEAAEKTGAPPPLPHRITVVGAGVSGISLGRFAA
                     SRGVQTFVSDQKALREDQKNSLAEAGILWEEGHTPRALETDLLLLSSGISPQAPLVRE
                     ARDRGIAIKGELSFLRPYLRGRLVGITGSNGKSTTVTLLGHMLQREGRAVAVAGNVGT
                     PLGDVADRPYDIVVMELSSFQLHWSEGLPLECGAITNLDPDHIDWHGSYESYVAAKMN
                     IFCGLSSSGAIVAQSREWASIPEGERHRVLLLGEEGDPRASLVLSSRGAILRRGHGST
                     VLFSRDDLPLLGRHNEENAAMAMALAVLLGEDPEVLRPRMATYSPLPHRCAFVTRWED
                     VTFVDDSKGTNVAASVTALRSLEGPKVVILGGRGKGESYDSLAKAVKECARWALIMGE
                     ERPRLLEALHRGGVEGVTEVSGMEEAVQEAFSRALP"
BASE COUNT          366 a          579 c          787 g          474 t
ORIGIN      
        1 ctgcggtgtc tttggccctt ttgggaatgg tgctccaagg acgatggatc acccttcttc
       61 agggttggcg ggcggagcag gtcatgaaag attacggtcc tcacgccgac agggagagta
      121 agatgggcat tcccagcgtg gggggggcgg tcttcctcct gttggggctg gggggatggg
      181 gatgggggct atgtcagatt ccgggggcgt cggaggaaca cctggtggtc tggggtctgg
      241 ctttaggggc atccctagtg ggctttctcg acgacttctt gaagatttcc cgcaaatcca
      301 gtgaaggact gagttccagg cagaaatttc tgtggcagat ggcggtgtgc cttccctggg
      361 ctttccacgt ggtggccctt cggggatggg gaggatcggt ggcttcggaa gccctgctgg
      421 tgatgggggt caccctggga gccaatggca tgctgaatgc ggtgaacgtc accgatgggt
      481 tggatggcct ggcggccgga tcctgtgccc tttcctttgt cgcccttgcg gggatggttc
      541 ctacgggatc tcccgcctgg tggggggcct tgatgggtgg aggtatcgcg acggggtttt
      601 tgtggcacaa cgcccacccc gcccgggtgt tcatggggga cggaggggcc catttcctag
      661 gagggcttct cttgggagtg gcggccttcg ggcgatcccc catggccgtg gtggccgtgt
      721 cccccctttt cggagtcgag atcctttccg tggcgataca gatcgtggcc cttcgctgtt
      781 ttcaccgtcg agtgtttcgt atgagccccc tgcaccatca ctttgagctg ggcggatgga
      841 aggagacaca gattgtcacg aggtttatgg tcctccacgc gatgggcatc gtcgttttag
      901 ggtccctggg ggggtgtttt aattgagagg gagtatggga gtgagtcatg tcacggaagc
      961 ggcagaaaag acgggggcgc ccccacccct tccccaccgg attacggtgg tgggggcggg
     1021 ggtgagcgga atttccctgg ggcgttttgc ggcgtcccgt ggggtccaga cctttgtcag
     1081 tgaccaaaaa gcactgcgag aagaccagaa gaactccctt gcggaagcag gcattctctg
     1141 ggaagaggga cacactcccc gggcccttga gacggacctg ctcctactga gctcagggat
     1201 atctcctcag gcgcccttgg tacgagaggc ccgagatcga ggcatcgcga taaagggaga
     1261 gttgagtttc ctgaggccct accttcgagg ccgtctggtg ggcatcacgg ggagcaacgg
     1321 aaaaagtacc acggtcacgc tcctggggca tatgcttcag cgggaggggc gggccgtggc
     1381 ggtggcgggg aatgtgggca cgcccctggg ggatgtggcc gaccgcccct atgacatcgt
     1441 cgtcatggag ttgagcagct tccagctcca ctggagcgag ggtctacccc tggagtgcgg
     1501 cgccattact aacctggatc cagaccatat cgactggcat gggtcttatg aaagctatgt
     1561 cgcggctaaa atgaatattt tctgcgggct ctcttcctcg ggggccatcg tggcccagtc
     1621 ccgggagtgg gcgtccattc cggaggggga gcgccatcgg gtcctgcttc tgggggaaga
     1681 gggcgatccc cgggcttcct tggtcttgtc ttcccgggga gccatcctcc gtcgggggca
     1741 cgggtccacc gtgctctttt ccagagatga tctgcccctc ctggggcgcc acaacgaaga
     1801 gaacgctgcc atggccatgg cactggcggt gcttttgggg gaggatccag aggtccttag
     1861 gccccggatg gcaacctata gccccctccc tcatcggtgt gcctttgtga ctcgatggga
     1921 agatgtaact tttgtcgacg actccaaggg aaccaacgtg gccgccagcg tgacggcgct
     1981 ccggtcattg gaggggccca aggtggttat cctggggggg aggggcaagg gggagagcta
     2041 cgactctctt gccaaggcgg tgaaggagtg tgcccggtgg gcgttgatca tgggagagga
     2101 gcgcccaaga cttttggagg cccttcaccg aggaggagtg gagggggtaa cagaggtctc
     2161 cggcatggaa gaggctgtcc aggaggcctt ttcccgggcc cttccc
//
LOCUS       DAOOER010000167         2195 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_48942_length_2195_cov_8.197196, whole genome shotgun sequence.
ACCESSION   DAOOER010000167 DAOOER010000000
VERSION     DAOOER010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48942_length_2195_cov_8.197196"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2194)
                     /locus_tag="PL162_10215"
     CDS             complement(<1..2194)
                     /locus_tag="PL162_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HQF92435.1"
                     /translation="MWQFFTERGKRVVQLAHREALSLGHDVIGTEHLLMGLLAEGEGV
                     AFQVLKRAAITLEDVRLRVEGAVGRGQPKIKPVDLPLSPRGKRVLEMAMREARNLGVN
                     YVGTEHILLGLLGEGEGMAAQILATMGIDLHRLSQEVTKALSSGESLGEAPGPEEPGG
                     KAPRQKTPTVDQICIDLTEMASRGELDPVIGRAREIQRVIQILSRRRKNNPVLIGDPG
                     VGKTAIVEGIAQRVLSGDLPESLRDKRVVMLNVGNLVAGTKYRGEFEERMRKLVKELR
                     EARDVILFIDEVHTIIGAGGAEGAVDAANILKPSLARGEFQVIGATTLDEYRKHIERD
                     AALERRFQPVLVNEPNQEDAIAILKGLRDAYEAHHRVKIGDDALEAAVRLSSRYISDR
                     YLPDKAIDCVDEAAARARLQTMEAPEDLRQMERDLEPVRAEKEAAAARQDFEAAARLR
                     DEERLISEALERRRKEWQMARSQEEPVITGEHIAHIVAEWTGIPVVQMTEEEAARLLR
                     MEEEIHKRLIDQVDAVGAVARAIRRARSGLKDPKRPAGSFLFLGPTGVGKTELARALA
                     EFLFGSEDALVRLDMSEFMERHEVSKLIGAPPGYVGYEEGGKLTEAVRRRPYSVVLFD
                     EIEKAHPDAFNLLLQILEDGRLTDGHAHAVDFRNTVVILTSNVGATDMLKGRGLGFVS
                     VDGVFGDWSRTEGRLLDEVKRTFRPEFLNRIDDIIVFKPLERSQLLQIV"
BASE COUNT          305 a          753 c          723 g          414 t
ORIGIN      
        1 ccacgatctg gaggagctgg ctccgctcca agggcttgaa gacgatgatg tcgtcgatgc
       61 ggttgagaaa ctccgggcgg aaggtgcgtt tcacctcgtc gaggaggcgc ccttcggtgc
      121 ggctccagtc gccgaagacc ccgtcgacgg agacgaagcc caggccccgc cccttcaaca
      181 tgtccgtggc ccccacgttg ctggtgagga tcaccacggt gttccggaaa tccacggcgt
      241 gggcgtggcc gtcggtgagg cggccatctt ccaggatctg gagcagcagg ttgaaggcgt
      301 cggggtgggc cttctcgatc tcgtcgaaga ggaccacgga gtaggggcgc cgccgcacgg
      361 cttcggtgag ctttcccccc tcttcgtatc ccacgtaccc cgggggggcg ccgataagtt
      421 tggacacctc gtggcgctcc atgaactcgc tcatgtcgag gcgcaccagg gcgtcttcgc
      481 tgccgaagag gaactcggcc agagcccggg cgagctcggt cttgcccacc cccgtgggcc
      541 ccaggaaaag aaagctcccc gcagggcgct tggggtcttt caatccgctg cgggcccgac
      601 ggatggcgcg ggccacggcc cccacggcat ccacctggtc gatgaggcgt ttgtggatct
      661 cttcttccat ccggaggagg cgcgccgctt cttcttcggt catctgcacc acggggatgc
      721 cggtccactc cgccacgatg tgggcgatgt gctccccggt gatgacgggc tcttcttggc
      781 tccgggccat ctgccattcc ttgcgccgtc gctccagggc ctcggagatg aggcgctctt
      841 cgtcccggag ccgggcggca gcctcgaagt cctggcgggc ggcggcggcc tccttttccg
      901 cgcgcacggg ctccaggtcg cgctccatct ggcggaggtc ttcgggggcc tccatggtct
      961 ggagccgggc tcgggcggcg gcctcgtcca cgcagtcgat ggccttatcg gggaggtagc
     1021 ggtcggagat gtatcggctg gaaagcctca ccgccgcctc cagggcgtcg tctccgatct
     1081 tcacccggtg gtgcgcctcg taggcgtccc ggaggccctt caggatggcg atggcgtcct
     1141 cttggttcgg ctcgttcacc agcacgggct gaaagcgccg ttccagcgct gcgtcccgct
     1201 cgatgtgttt gcggtactca tccagggtgg tggcgccgat gacttgaaac tccccccggg
     1261 ccaggctggg cttgaggatg ttggccgcat ccacggctcc ttcggccccc cccgccccga
     1321 tgatggtgtg cacctcgtcg atgaagagga tcacgtcccg ggcctcgcga agctccttca
     1381 cgagcttgcg catgcgctct tcgaactctc cccggtactt ggtgcccgcc acgaggttgc
     1441 ccacgttgag catgaccacc cgcttgtccc ggaggctctc gggtaggtca ccggagagca
     1501 cccgctgggc gatgccctcc acgatggcgg tcttgcccac gccggggtcg cctatgagga
     1561 cggggttgtt ttttcggcgc cgggaaagga tctggatcac ccgttggatc tcccgggccc
     1621 ggccgatgac ggggtccagc tccccccggc tggccatctc ggtgaggtcg atgcagatct
     1681 gatccacggt gggggtcttc tggcgcgggg ccttgccccc gggctcctcg gggcccgggg
     1741 cctcccccag ggactccccc gaagagaggg ccttcgtcac ctcttgggag agcctatgga
     1801 ggtcgatgcc catggtggcc aggatctggg ccgccatgcc ctctccttct cccaggaggc
     1861 ccaggaggat gtgctccgtg cccacgtagt tcactcccag gtttcgggcc tcccgcatgg
     1921 ccatctccag cacccgcttg ccccggggcg ataggggcag gtccacgggc ttgatcttgg
     1981 gctgccctcg ccccacagcg ccctccaccc ggaggcgcac gtcctccaag gtgatggcgg
     2041 cccgtttaag tacctgaaag gccaccccct ccccctcggc caaaaggccc atcaggaggt
     2101 gttccgtgcc gatgacgtcg tgtcccaggg aaagggcttc ccggtgcgcc aactgcacca
     2161 ctcgctttcc ccgttcggta aaaaactgcc acatg
//
LOCUS       DAOOER010000168         2190 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_49090_length_2190_cov_8.098361, whole genome shotgun sequence.
ACCESSION   DAOOER010000168 DAOOER010000000
VERSION     DAOOER010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49090_length_2190_cov_8.098361"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(428..715)
                     /locus_tag="PL162_10220"
     CDS             complement(428..715)
                     /locus_tag="PL162_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HQF92436.1"
                     /translation="MADKWKDRLTDQLCRALLSLKDREEVYCLLEDIATIGEIKALAQ
                     RLEVARLLSEGHTYPEIAKETGASTATISRVKKFLEYGADGYKLVLDRIKE"
     gene            complement(923..2023)
                     /locus_tag="PL162_10225"
     CDS             complement(923..2023)
                     /locus_tag="PL162_10225"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HQF92437.1"
                     /translation="MATLSSIGSPSPLQGEIRLDANESPFALPPGPREALRELLAAQE
                     FHRYPDPNARELRGALASRWGVAPENVAVGNGADEVLLNLFLAFVRPGDVVLRLDPCF
                     SQYPRLCRLFGATERRVPVEWREGRFSVSEEHILDAVAATSPKLVLLDTPHNPTGVTI
                     GVEFLRKLGEISPSPVVVDEAYGEFATETVLPGVATRGIPRQAVLVRTLSKAWGLAGL
                     RLGYAVARPEMIEKMDAVRSPFNVGVWPQAAAKLLLSFDEWMESRVLSLTYIRDRFVE
                     AVNGLGKGWIAAPSEGNFVLVHTPQERGLVQEVLRQAGLCVKFFDDLPWKGCWMRVTV
                     GKEEDMRRVVETFSSVTVSRGEDEDLLAPASA"
BASE COUNT          465 a          723 c          566 g          436 t
ORIGIN      
        1 cggcgctgcg tctcgtgcat gtgctgccac gaaatgtgcc ctccgggggc catgaaatgc
       61 cagccctccc ccctggctcg cctcatgctc tggtggcgtc ctccctcgag aggacgcacc
      121 acgaaggctt cctcaggacc ttcatgacgg ggctgtcaaa cgcccccggc ccgtcccttt
      181 taaccccctg accggcccga taaacccgac agcggtgtca aagggcctcg gaggccgtaa
      241 taatcatcga gagaaagggt cttccgatcc cctaggctag gtccatccct ggaccctgcg
      301 cacggaaggg gcccgcttcc agggtaagga agcgggcccc ttccgtgaca cggcgaaggt
      361 ctttcagtcg ggtttgtttg ctacgccaac tctcggagca ggtctcctgg aaacgccccc
      421 aggcttccta ctccttgatg cggtctagca ccagcttata tccgtcggcg ccgtactcca
      481 ggaatttctt cacccgactg atcgtcgctg tgcttgcccc cgtctccttg gcaatctcag
      541 gataggtgtg tccctcgctc agaagccgag ccacctccag acgctgcgcc agggccttga
      601 tctccccgat ggtggcaatg tcctccagaa ggcagtagac ctcttcccgg tctttcaaag
      661 acaggagcgc cctgcaaagt tggtcggtca atcgatcctt ccatttatcc gccatcacgc
      721 aattcctccc ttacgttcga gaaggattct tactcccctc ctctttctcg tcattataac
      781 acgacaatcc cccggagctc aagcactctc ttagacaagg cagtctgctt tccaaaaaga
      841 acttcgccgc cgtgacggtt aagcccacag gcatgatacc cccaccaccc atcggccatc
      901 gccaagttga cgtaatgcgg ccttaggcgg acgccggcgc gaggagatct tcgtcctctc
      961 ctcgggagac ggtcacgcta gagaaggtct ccaccacccg gcgcatgtcc tcttcttttc
     1021 ccacagtgac gcgcatccag catcccttcc agggcaaatc atcaaagaac ttcacgcaaa
     1081 ggcccgcctg tcgcaggacc tcctgaacca agcccctctc ttgaggagtg tgcaccagga
     1141 caaaattgcc ctcgcttgga gcggctatcc atccctttcc caggccgttt accgcttcca
     1201 cgaagcgatc cctgatataa gtaaggctca acacgcggct ttccatccac tcgtcgaaag
     1261 acagaaggag cttcgccgca gcttggggcc agactcccac gttgaaaggg ctgcgcaccg
     1321 cgtccatttt ctctatcatc tccgggcggg ccaccgcata tcccaggcgg agccccgcca
     1381 gtccccaggc tttagacagg gtgcgcacca gcaccgcttg gcgaggaatg cctcgcgtcg
     1441 ccacgccggg caacaccgtc tcggtcgcga actctccgta ggcctcgtcc acgacgacag
     1501 ggctagggga aatctctcca agctttcgca aaaactccac gccgatggtc acccctgtgg
     1561 ggttatgggg ggtatccaac aggacaagct taggggacgt cgccgccaca gcgtctaaaa
     1621 tatgttcctc gctgacggaa aaacgcccct ctctccactc cacaggcact cgacgctccg
     1681 tggcaccaaa gaggcgacac aggcgaggat actgggaaaa gcagggatcc aggcgcaaaa
     1741 ccacgtcgcc cgggcgaaca aaggcaagaa aaaggtttag taacacctcg tccgcgccat
     1801 ttcccacagc cacgttctcc ggagccaccc cccagcgcga cgccaaggcc ccccgaagct
     1861 cccttgcgtt cggatcggga taccggtgga actcttgcgc cgccagaagc tctcgcaggg
     1921 cctcgcgagg tccgggggga agggcaaagg ggctttcatt agcatcaagc cgtatttcgc
     1981 cctgtagggg ggacggggaa ccgatggaag aaagggtcgc catgggtcgc aaagagatct
     2041 cctcctctat tactttagca agataaattg atttttgttc agcggttgca gattaccaca
     2101 taacatccat gttgtcaaca tgtcgaagaa aaatttgtga ccagcctgtg gtattgtcat
     2161 tcctgaacgg gacgtggata atgtccatca
//
LOCUS       DAOOER010000169         2155 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_50297_length_2155_cov_6.915238, whole genome shotgun sequence.
ACCESSION   DAOOER010000169 DAOOER010000000
VERSION     DAOOER010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50297_length_2155_cov_6.915238"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PL162_10230"
     CDS             complement(<1..519)
                     /locus_tag="PL162_10230"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HQF92438.1"
                     /translation="MIALREDRLEGEILYRGRILTLRRDRVRLPQGKEALREVVDHAV
                     AVGVVAENDRGEILLVRQFRYAVGEELLEIPAGLVEPGEGVLETAHRELREETGFDAE
                     EMVPLGMFYSSPGFCNEEIVLFLGRHLKPDPRPQDDDERIVLCPTPRQEVKRLLREGR
                     LKDGKTLVALHQY"
     gene            complement(516..1073)
                     /locus_tag="PL162_10235"
     CDS             complement(516..1073)
                     /locus_tag="PL162_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HQF92439.1"
                     /translation="MAQFSRGLLAAGFGGQGILTLGQLVAYTAMNEGRFVTWIPSYGP
                     EMRGGTANCSVVVSDAEIASPVVNHADAVVIMNQPSLEKFEKMVKPGGVLIYNSDLVT
                     YKNPRTDVKVIPVPANSAAAEMGNDKVANVIALGALAEATDLMKPETCLETLKEKLGK
                     KKAQLLELNINAFARGREIARDASR"
     gene            complement(1087..1830)
                     /locus_tag="PL162_10240"
     CDS             complement(1087..1830)
                     /locus_tag="PL162_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013048404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQF92440.1"
                     /translation="MKETKIFEKPESMTDVITHYCPGCTHGVAHRLVAETIDELGIRE
                     RTFGVAPVGCSVFIYNYIDVDMYEAAHGRAPAVASAAKRIRPDMVGFTYQGDGDLASI
                     GMAEIVHAANRGENITVIFINNAIYGMTGGQMAPTTLFGQKATTCPLGREASVNGYPI
                     RIAEMLATLASPGYIARVSVSKPKYVLKAKEAIKKAFQTQIDKKGFSLVEVLSTCPTN
                     WGLRPVEATDWLEQHMMPYYPLGEFKLPE"
     gene            complement(1845..>2155)
                     /locus_tag="PL162_10245"
     CDS             complement(1845..>2155)
                     /locus_tag="PL162_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HQF92441.1"
                     /translation="VLTAYGTTARIARSAIMNLRSQGLKVGMLRPISLFPFPYAPFEN
                     LPASVRHVLVVEMSCGQLIDDIKIGVGRAVPISFHGRVGGMCPTVEEIEEKIKSVIGK
                     "
BASE COUNT          379 a          670 c          659 g          447 t
ORIGIN      
        1 gtactggtgg agggccacca gggtcttccc gtccttcagg cgtccctccc ggagtaggcg
       61 cttcacttct tggcgagggg tggggcagag cacgatccgc tcgtcgtcgt cctggggtcg
      121 gggatcgggc ttcagatgac gtcctaggaa gagcacgatt tcttcgttgc aaaatcccgg
      181 ggatgagtaa aacatcccca gggggaccat ttcttcggcg tcaaagcccg tctcctctcg
      241 aagctcccgg tgggcggtct ccagcacgcc ctctccgggt tccaccagcc cggcggggat
      301 ttccaggagt tcttctccca cggcataacg gaactggcgc accagaagga tctctccccg
      361 gtcattttcc gccaccaccc ccacggctac ggcgtggtcc accacctctc gcagggcttc
      421 tttcccctgg ggaaggcgca cccgatcccg gcgtagcgtg aggatgcgcc cccgatagag
      481 aatctctcct tccagacggt cctcacgaag cgcgatcacc gggaggcgtc gcgggcgatc
      541 tctcgccccc gggcgaaggc gttgatgttg agctccagca actgggcctt cttcttgccc
      601 agcttttctt tgagagtttc cagacacgtt tcgggcttca tgaggtccgt ggcctctgcc
      661 agggccccca gggcgatgac gttggccacc ttgtcgttgc ccatctcggc ggcggcactg
      721 ttggcgggca cggggatgac cttcacgtcg gtgcgggggt ttttgtacgt cacaaggtcg
      781 gaattgtaaa tcaacacgcc cccgggtttc accatctttt cgaatttttc cagcgaaggt
      841 tgattcatga taacgacggc atcggcgtgg ttgaccaccg gagatgcgat ctccgcatcg
      901 ctgaccacca cgctgcaatt tgccgtccct ccgcgcattt cagggccgta ggaggggatc
      961 caggtgacaa agcgcccctc gttcatggcc gtgtaggcca cgagctgccc tagggtcagg
     1021 atgccctgac ctccaaagcc agcggcgagc aagccgcggg aaaactgagc catggcgtcg
     1081 tcccccttac tccggaagct tgaattcgcc caaaggatag tagggcatca tgtgctgctc
     1141 cagccaatcc gtggcctcca cggggcggag gccccagttg gtggggcagg tggagagcac
     1201 ttccaccagc gaaaagccct ttttgtcgat ctgggtctgg aaggccttct tgatggcctc
     1261 cttggctttg agcacgtact tgggcttgga caccgatacc cgggcgatgt agccgggcga
     1321 ggccaacgtg gccaacatct ccgcgatccg gatggggtag ccgttgacgc tggcttcccg
     1381 tcccagagga caggtggtgg ccttctgtcc gaagagggtg gtgggggcca tctgcccccc
     1441 cgtcatgccg taaatggcgt tattgatgaa aatcacggtg atattctccc cccggttggc
     1501 ggcgtgcacg atctccgcca taccgatgga ggccaggtcc ccatctcctt ggtaggtgaa
     1561 tcccaccatg tcggggcgga tgcgcttggc tgcgctggcc acagcgggag cccggccatg
     1621 ggccgcctcg tacatgtcca catcgatgta gttgtagatg aagaccgaac atcccaccgg
     1681 cgccacgccg aaggtgcgct cccggatacc cagctcgtcg atggtctccg ccaccaggcg
     1741 atgggccacc ccgtgggtgc agcccggaca atagtgcgtg atgacatcgg tcatggattc
     1801 aggtttttcg aaaatttttg tttctttcac cgtccccacc ccccctactt gccgataacg
     1861 gattttatct tttcttcaat ctcttccacg gtggggcaca tgccccccac tcggccgtgg
     1921 aaagagatgg gcacggcgcg ccccacgccg atcttgatgt catcgatgag ctgaccgcag
     1981 ctcatctcca ccaccagcac atggcgaacg ctggcgggaa ggttctcgaa gggggcgtag
     2041 gggaagggga agagggatat ggggcgcagc atgccgactt ttagcccctg gcttcgaagg
     2101 ttcatgatgg cggaacgggc gatgcgcgcc gtggtgccat aggccgtcag cacga
//
LOCUS       DAOOER010000170         2154 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_50337_length_2154_cov_7.535970, whole genome shotgun sequence.
ACCESSION   DAOOER010000170 DAOOER010000000
VERSION     DAOOER010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50337_length_2154_cov_7.535970"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PL162_10250"
     CDS             <1..224
                     /locus_tag="PL162_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HQF92442.1"
                     /translation="AKESNNELVVELVSEGNNKEGSDKGAPGEEDQMLLSELQEKRRQ
                     EGTLRTFRSNYYIEGLLRRSLKLTPSKGS"
     gene            287..1057
                     /locus_tag="PL162_10255"
     CDS             287..1057
                     /locus_tag="PL162_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQF92443.1"
                     /translation="MAWIGRQVLAFLSSLGSFALYGRSMARGIFRGRWEWHDFFDHMD
                     RIGVGSLFMVTVTSAFSGMVMAVQTLDQFVRFGATQYVGGVIALTMIREMAPVLTGLV
                     VTGRVGAAMAAEIGTMNVTEQLDALRAFGMDHVSFVGTPRLLASALMLPVLTIFSFAV
                     SVGGAGLYVTTHGISGFVFRRSVSVMVHHIDLAGGLVKALAFGILIAVSSCTEGFRAE
                     NGARGVGFATTRAVIWSNMLILIFNYIISTIFFGNFFR"
     gene            1066..1845
                     /locus_tag="PL162_10260"
     CDS             1066..1845
                     /locus_tag="PL162_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQF92444.1"
                     /translation="MDQGRIVSLSQVGMDFNGHTVLHEVSFDLYPGEIVALMGPSGCG
                     KTTVLRILIGLLEASRGELEIFGSPVREDTSRELLVSLRRRMGVVFQNGALFDSLTVG
                     ENVMFPLRHCLGMRDRDAMKIRAQEILGAVELPGVEEKYPSELSGGMRKRVAIARSLV
                     YRPELLLLDEPTTGLDPQTARHVDRLVRDLGRRFGIGVLVVTHDLVTTLGIAHRILLI
                     DQGRISWRGSVEEWRRSTSPEVRRFARGMEPVNQREENPDA"
     gene            1838..>2154
                     /locus_tag="PL162_10265"
     CDS             1838..>2154
                     /locus_tag="PL162_10265"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HQF92445.1"
                     /translation="MRETKVGAVVFLALTLLGGMVFLTGGAFFSKKGYTLHVLFSDAR
                     GLVPGAPVFVSGVERGTIRELAVEPEGVRASLLVAPEVLIPEDSVFTIATSGLLGNPS
                     IKVT"
BASE COUNT          394 a          613 c          687 g          460 t
ORIGIN      
        1 ctgccaaaga gtcaaacaac gagcttgtgg tagagttggt cagtgaagga aacaataaag
       61 agggatcgga caaaggagct cccggggaag aggatcagat gctcctgagc gagctccagg
      121 aaaagcgtcg ccaggaagga accctgcgca ccttccggtc aaactactac atcgaagggc
      181 tcctacgccg ctcgctgaaa ctgaccccct ccaagggatc gtgacgtcga gggggaggac
      241 gtcgtcggag ccctcttgcg ggaaaggagt ggggcccgtg gggctgatgg cctggatcgg
      301 ccgacaggtc ctcgcgtttt tatcgtctct agggtcgttc gccctttacg gccggtccat
      361 ggcccggggc attttccgag ggcgctggga atggcatgat tttttcgacc acatggaccg
      421 gatcggcgtg gggtcccttt tcatggtaac ggtgaccagc gccttctccg gcatggttat
      481 ggcggtccag accctggacc agttcgttcg gttcggtgcc acccagtatg tgggaggcgt
      541 gatcgcccta accatgataa gggaaatggc cccggtcctc acagggctcg tggtaacggg
      601 gcgcgtggga gcggccatgg cggcggaaat aggtaccatg aacgtcaccg aacagttgga
      661 cgccctaaga gccttcggca tggaccacgt gtccttcgtg ggcacgcccc gactgttggc
      721 cagtgccctc atgcttcccg tgctcacaat cttctccttc gcggtgagcg tgggaggagc
      781 ggggttatac gtcaccaccc acggtatcag cggcttcgtc tttcgccgct cggtgtccgt
      841 gatggtccac cacatcgacc tcgcgggggg cctcgtgaag gccctggctt tcggcatcct
      901 aatcgctgtt tcctcctgta ccgaagggtt ccgggcggaa aacggggccc gaggggtggg
      961 ctttgccacc acccgggccg tcatctggag caacatgctc atactgattt ttaattacat
     1021 tatttccacc atattttttg ggaatttttt ccggtgatgg gcgagatgga tcaaggacgg
     1081 atcgtctccc tgtctcaggt ggggatggat ttcaacggtc acacagtgct acacgaggtg
     1141 tcgttcgacc tctatcccgg cgagatcgtc gccttgatgg ggccctcggg ttgcgggaaa
     1201 accaccgtgt tacgcattct cataggcttg ctcgaggcct cccggggaga gcttgaaatt
     1261 tttggatctc ccgtccgcga ggatacttcc cgggaactgc tggtatccct ccggcgccgg
     1321 atgggggtgg tgtttcaaaa cggcgctctc ttcgactcct tgacagtggg agagaacgtc
     1381 atgtttcccc tgaggcattg ccttggcatg cgcgatcggg atgccatgaa gatccgagct
     1441 caggaaatcc ttggggccgt cgagttgccg ggagtggagg aaaagtaccc ctccgagctc
     1501 tccgggggga tgagaaagcg ggtggccatc gcccgatccc tggtgtaccg ccccgaactg
     1561 ctcctgctgg acgagcccac caccggcttg gatccccaaa cagcgcgtca cgtggaccgc
     1621 ctggtgagag atctagggcg gcgctttggc ataggggtgt tggtggtaac ccatgacctg
     1681 gtgaccacgc tgggcatcgc ccaccgcatc ctgctcattg accaggggag aatttcttgg
     1741 cggggctccg tggaggaatg gcgccgatcg acctctccgg aagtccggcg ttttgcccga
     1801 ggcatggagc ccgtgaatca aagggaggaa aaccccgatg cgtgagacaa aggtgggggc
     1861 cgtggtcttt ttggccctga cgcttctggg aggcatggta tttctcacgg gaggcgcttt
     1921 tttttccaaa aaaggctata cccttcacgt gctcttctcg gacgcccgag gcctcgtccc
     1981 cggagccccg gtgttcgtat ccggggtcga gcgggggacc attcgcgagc tcgccgtgga
     2041 gcccgaaggc gtcagggcta gcctgctggt tgccccagag gtccttattc ccgaggatag
     2101 cgtcttcacc atcgccacct cggggctact ggggaacccc agtatcaaag tcac
//
LOCUS       DAOOER010000171         2136 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_50990_length_2136_cov_8.850553, whole genome shotgun sequence.
ACCESSION   DAOOER010000171 DAOOER010000000
VERSION     DAOOER010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50990_length_2136_cov_8.850553"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..417)
                     /locus_tag="PL162_10270"
     CDS             complement(10..417)
                     /locus_tag="PL162_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006302046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HQF92446.1"
                     /translation="MRRRKKDVRVYPALLSEEGEFVNVRFPDLPGCNTFGNGYADAVA
                     SAKDALGGHLVCMEDDHDPIPEPTPVNKLHPGEGEVAVLVDVRMDILREEESQKSVSK
                     NVTIPKWLNELATEAGVNFSSTLQEALRERLGV"
     gene            complement(448..678)
                     /locus_tag="PL162_10275"
     CDS             complement(448..678)
                     /locus_tag="PL162_10275"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HQF92447.1"
                     /translation="MRVVLFLSGGWSKSAARKTSSNSKQLEADGWILIKVKGSHHHFK
                     HHAKPGKVTVKHPQKDVPEGTRQNILRQAGLK"
     gene            722..1024
                     /locus_tag="PL162_10280"
     CDS             722..1024
                     /locus_tag="PL162_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HQF92448.1"
                     /translation="MTAFFVGVTRVEFGQPLRSQREVVAVRDALERRCLRDGGLFVLG
                     TNTGLRISDLLRLRVSDVLTPGARRRQVASRLQIREKKTGKAKDIPLNAACRSPLW"
     gene            1089..1571
                     /locus_tag="PL162_10285"
     CDS             1089..1571
                     /locus_tag="PL162_10285"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HQF92449.1"
                     /translation="MTISQRKMRRKDKEISDPREVEAILKEALTLHLGLCLGDEPYVV
                     PMTYGYAEGKIYLHGGVGGTKFELMAANPRVSFSVVTGVETIPHPEPCEWSVRYRSVV
                     GFGTARFLETPEEKNRGLNALTAHLGAGTHAYSPELLARVVVVEIDVTQATGRQSGYP
                     "
     gene            complement(1618..2121)
                     /gene="ybaK"
                     /locus_tag="PL162_10290"
     CDS             complement(1618..2121)
                     /gene="ybaK"
                     /locus_tag="PL162_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008519710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HQF92450.1"
                     /translation="MADKKTNAVRLVEDRGFACELRRYAVDEDDLGAENVASKVGLPL
                     AQVFKTLVARGDRTGVVVACVPGDEELDLRKLAAASGDKRAGLVPLKELQPLTGYLRG
                     GCSPVGMKKGYPTFLDQSAFDHPCIAVSAGARGIQMILDPHDLQALTGARVADLCVRP
                     KETLPPP"
BASE COUNT          351 a          599 c          733 g          453 t
ORIGIN      
        1 tgctgtgggt cagaccccga ggcgttcccg cagggcctcc tgcaaggtgc tggaaaaatt
       61 tacgcctgct tctgtggcca gttcgttgag ccacttgggg atcgttacgt tcttgctgac
      121 ggatttctgg gattcctcct cgcggaggat gtccatgcgg acgtccacca gcacggccac
      181 ctccccttcc ccgggatgga gcttgtttac gggggtcggc tcggggatgg ggtcgtgatc
      241 gtcctccatg cagaccaaat gcccgcccaa ggcgtctttc gcgctggcga cggcgtcggc
      301 gtatccattc ccgaaggtgt tgcacccggg gagatcgggg aagcgcacgt tgacgaactc
      361 cccttcttcg gaaagcagtg cggggtagac ccttacgtct tttttccttc ttctcatggt
      421 ctatcgttcc tcccttgcgg gcggggccta tttcaacccc gcctgccgca ggatgttctg
      481 tctggtgcct tccgggacgt ctttctgggg gtgcttcacc gtgaccttgc ccggcttggc
      541 gtgatgcttg aagtggtgat ggcttccctt gactttgatc agaatccagc cgtctgcttc
      601 aagctgtttg ctgtttgatg atgtctttcg agctgctgat ttcgaccacc ccccggacag
      661 aaagagtaca acgcgcataa tcatgcgcgt caatgggggc gggggaaata agttttggga
      721 ggtgacggct ttttttgtgg gggtgacccg ggtggagttt gggcaaccgc ttcggtctca
      781 gcgtgaggtg gtggcggtac gcgatgcgtt ggagcgtcgg tgtctccggg acgggggcct
      841 cttcgttttg gggacaaaca cgggattgcg gatctcggat ctgttgcggc tccgggtttc
      901 ggacgtcctc acgccggggg cgcgtcggcg ccaggtggca tctcgacttc agattcggga
      961 aaagaagacg ggcaaggcca aggatatccc cttgaatgcc gcatgccgat ctcctttgtg
     1021 gtaggatggg agggataatt tcggggaggg gcggatcccg cctcaccggg aagtggggag
     1081 gaatggcggt gacgatatcg cagagaaaga tgaggcgcaa ggacaaggag atttctgacc
     1141 cccgagaagt ggaggcgatc ctcaaggagg ccttgacgct ccacctgggg ctgtgtcttg
     1201 gggacgaacc ctatgtcgtg cccatgacgt acggctatgc cgaggggaaa atctacctgc
     1261 atggaggagt ggggggaacg aagttcgagt tgatggcggc gaacccccgg gtttctttca
     1321 gcgtggtgac gggggtggaa acgattcccc atccggagcc ctgcgagtgg agcgtgcggt
     1381 accgcagcgt cgtgggcttt ggaacggcgc gattccttga aactcccgag gaaaaaaatc
     1441 ggggcctgaa cgccctcacc gcccacctgg gggccggaac ccacgcctat tccccggagc
     1501 ttctcgcccg ggtcgtggtg gtggaaatcg acgtgaccca ggcgacggga cgccagtcgg
     1561 gctatccctg agggctcctt cggggggacc gagggaaggc gaggggcggc tcgggggcta
     1621 gggcggggga agggtttcct tggggcggac gcacagatcg gcgacccggg ctccggtgag
     1681 ggcctggagg tcgtgggggt ccaggatcat ctgaatcccc cgggctccgg cgctcaccgc
     1741 gatgcagggg tggtcgaagg ccgattgatc caaaaaagta gggtagccct ttttcatgcc
     1801 cacgggggag cacccccctc ggagatatcc cgtgaggggt tgcagctcct tcaggggcac
     1861 gagccctgcc cgtttgtcgc cgctggcggc ggcgagcttg cggaggtcga gctcttcgtc
     1921 ccccggcacg caggctacca ctacgccggt gcggtctccc cgggccacta gggtcttaaa
     1981 gacctgggcc agggggagtc ccaccttgga agccacgttt tccgccccca ggtcatcttc
     2041 gtccacggcg tagcggcgga gctcgcaggc aaaacctcgg tcttccacca ggcggacggc
     2101 gttggtcttt ttgtctgcca tgacatcacc tctttc
//
LOCUS       DAOOER010000172         2095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_52490_length_2095_cov_7.928922, whole genome shotgun sequence.
ACCESSION   DAOOER010000172 DAOOER010000000
VERSION     DAOOER010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52490_length_2095_cov_7.928922"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            340..416
                     /locus_tag="PL162_10295"
     tRNA            340..416
                     /locus_tag="PL162_10295"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:374..376,aa:Arg,seq:acg)
     gene            841..1374
                     /locus_tag="PL162_10300"
     CDS             841..1374
                     /locus_tag="PL162_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HQF92451.1"
                     /translation="MRRGEWIVVLKAGRRGFGLVELVSVFVIGAIISGMAFALFSSGW
                     WYGGSSAAAWREAYRLARWISAAVRQSIQNGQGFTIYPPAGTMETRIRLTWRGRDGNP
                     LPYGEGDRWWTSDRSCYILSRGGSVAISNFIQRRAMFVPGFTVEVRHRRRLSDPPDCY
                     VIVSPYAYVTVSATPPP"
     gene            1527..1612
                     /locus_tag="PL162_10305"
     tRNA            1527..1612
                     /locus_tag="PL162_10305"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1561..1563,aa:Tyr,seq:gta)
     gene            1623..1698
                     /locus_tag="PL162_10310"
     tRNA            1623..1698
                     /locus_tag="PL162_10310"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1656..1658,aa:Thr,seq:ggt)
BASE COUNT          371 a          577 c          697 g          450 t
ORIGIN      
        1 gtggggtaaa aggggcgtgg aaggggaaac atttcgccta aaggtttggc cttgttgcct
       61 cccggggcga agggtggagt cggggcggcg ggcgctcttc ggccatggca ggctaaaaaa
      121 gccggcggga aggtgcgtgc tcttcggcca cggcgcatcc tccagcccgt tttctctagt
      181 tttttagtcc cgtagcccat cgcccttggt cgtcatgaat gacattttcc caggctagat
      241 caaggatgac cataccagag cctggccaca cagggccccc gggatccttg acggggtttt
      301 tggggcgtgg tacaatgacc gccgtcgtga ggagcgcgtg cgccggtggc tcaactggat
      361 agagcatcgg actacggatc cgaaggttac gggttcaaat cctgtccggc gcgccagacg
      421 gatgctccgg ggggcttagg ctagagcccc ccggtttttt ttgtgtcttc tccaaaagcc
      481 tcgccaggga ccccgccccc cggctccccc acggggcggc tgggatttcc ccgtgggggc
      541 aaggcttttt cctgcctcgc ctttttcccg tcgatttccc tgaaaccgcc tctttgcggt
      601 aaaatgacac cgaacgatat gatctttccg tcgggctagg ggctgcggag caaaacacgc
      661 tccgaagggg gcgccgtcgg cgcctttccc tggactgggg gcgacgaggg gcgtgggggc
      721 tggtggcctt gccgagaatc ccggcgacgg gtgcttccgt agggtctccg ataaggggga
      781 tcgcccgaag gaggccgccg acctatcagg tagcggaggt ggcgaagggg aggtagggag
      841 atgaggcgcg gggagtggat cgtggttctg aaggcggggc gtcggggttt cggtctggtg
      901 gaactagtga gcgttttcgt catcggagcc atcatttccg ggatggcctt cgcccttttc
      961 tcctcggggt ggtggtatgg ggggagtagc gctgcggcgt ggcgcgaggc ctatcgtttg
     1021 gcgcgctgga tcagcgcggc ggtgcggcaa agcattcaaa atggccaagg ttttaccatc
     1081 taccctccgg cgggaaccat ggaaaccagg atacgtctca cgtggcgggg ccgggatggc
     1141 aatcctctgc cctacggaga aggggaccgg tggtggacca gcgatcgttc ctgttatatc
     1201 ctctcccggg gaggatccgt ggcgatatct aattttattc agcgtagagc catgtttgtc
     1261 cccggtttta ccgtggaggt acgtcaccgc cgtcggctgt cggatcctcc agattgctac
     1321 gtcattgttt ccccctatgc ctacgtcact gtgtccgcca ctcccccgcc ctgatgattc
     1381 ctgtttccgt ccgcgctccg gggcggtgat atcccttgga atccccggga ttcgccgaaa
     1441 gaggatgggt ttgccccgaa gagcttgaca aaaactgtgg tgccgttgta tagttcccgt
     1501 tgcgccggtg gcgcggcgcc aagggaggta ggatggccga gtggtcaaag gcagcagact
     1561 gtaaatctgc cgacagacgt ctacgaaggt tcgaaccctt ctcctaccac catagagaac
     1621 cagccgactt agctcagcag gtagagcaca tccatggtaa ggatggggtc tccggttcga
     1681 gtccggaagt cggctccagc ttcggcaacg gccccttttc gacggtcgaa gaccagagaa
     1741 aggggcctgc ctttttcccc tcttcgtttc tccgacggga cgaaggccgg ccagggctct
     1801 gggaccggcc tttttgtgta tttttttgtt ccccctcccg cttgacgtgg gggcggtcaa
     1861 tgttacaata ccatgggcgg gagtggattt ccgagggcgt gcttctcggc gggagatgcg
     1921 ggaagggggc cttaggggcc ctctttggcc tgggaggagc aaaaagcagg catcacatcg
     1981 ccgccgccgg acaaaaccct tccggggggc gcaatctaaa gggaggtttc gcggatggca
     2041 aaggataagt ttgaacgaaa caagatgcac ctgaacattg gcaccatcgg tcaca
//
LOCUS       DAOOER010000173         2080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_53081_length_2080_cov_4.978272, whole genome shotgun sequence.
ACCESSION   DAOOER010000173 DAOOER010000000
VERSION     DAOOER010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53081_length_2080_cov_4.978272"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="PL162_10315"
     CDS             <1..800
                     /locus_tag="PL162_10315"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HQF92452.1"
                     /translation="IPSVGGAVFLLLGLGGWGWGLCQIPGATGEHLGVWGLALGASLV
                     GFLDDFLKLSRKSSEGLSSRQKFLWQMAVCLPWAFYVVALRGWGGSAASEVLLVMGVT
                     LGANGMLNAVNVTDGLDGLAAGACALSFVALAGMAPAGSPAWWGALMGGGIATGFLWH
                     NAHPARVFMGDGGAHFLGGLLVGVAAFGRSPMAVVAASPLFGVEILSVAIQIVALRCF
                     HRRVFRMSPLHHHFELGGWAETQIVTRFLALHALGIVVLGSLGGCFS"
     gene            815..>2080
                     /gene="murD"
                     /locus_tag="PL162_10320"
     CDS             815..>2080
                     /gene="murD"
                     /locus_tag="PL162_10320"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HQF92453.1"
                     /translation="MSHVTEASEETGAPQPLPRRITVVGAGVSGISLGRFAAVRGVQT
                     FVSDQKILREDQKNALAEAGIPWEEGHTPRALETDLLLLSSGISPQAPLVREARDRGI
                     AIKGELSFLGPYLRGRLVGVTGSNGKSTTVTLLGHMLQREGRAVAVAGNVGTPLGDVA
                     DRPYDIIVMELSSFQLHWSEGLPLECGAITNLDPDHIDWHGSYESYVADKMNIFRGLS
                     PSGAIVAQSREWASLPEGERHRVLLLGEEGDPRASLVLSPRGAILRRGHGSTALFSRE
                     DLPLLGRHNEENAAMAMALAVLLGEDPEVLRPRMATYSPLPHRCAFVARWEGVTFVDD
                     SKGTNVAASVTALRSLEGPKVVILGGRGKGESYDSLARAVKECARWALVMGEERPRLL
                     EALRRGEVEGVKEVSGMEEAVQEAFSRALP"
BASE COUNT          329 a          549 c          761 g          441 t
ORIGIN      
        1 gcatccccag cgtgggggga gcggttttcc tcctgttggg gctgggggga tgggggtggg
       61 ggctatgcca gattccgggg gctacggggg agcacctagg agtctggggt ctggctttag
      121 gggcgtccct ggtggggttc ctcgacgatt tcctgaagct ttctcgcaaa tccagtgaag
      181 gactgagctc caggcaaaaa tttttgtggc agatggcggt gtgtcttccc tgggctttct
      241 acgtggttgc ccttcgggga tggggcggat cggcggcttc ggaggtcctg ctggtgatgg
      301 gggtcacttt gggagccaat ggcatgctta atgcggtgaa cgtcaccgat gggttagatg
      361 gcctggcggc cggagcctgt gccctttcct ttgttgccct cgcggggatg gcccctgcgg
      421 gatcccctgc ctggtggggg gccttgatgg gtggaggtat cgcgacgggg tttttgtggc
      481 acaacgccca ccccgcccgg gtgttcatgg gggacggagg ggcccatttt ctaggagggc
      541 ttctcgtggg ggtggcggcc tttgggcgat ctcccatggc cgtggtggcg gcgtcccccc
      601 ttttcggagt cgaaatcctt tccgtggcga tacagatcgt ggcccttcga tgttttcatc
      661 gtcgggtgtt tcgcatgagc cctctgcacc accactttga gctgggcggg tgggcggaaa
      721 cgcagattgt cacaagattt ttggccctcc atgcgctggg catcgtcgtt ttagggtccc
      781 tcgggggatg ttttagttga gaggggggat gggagtgagt catgtcacgg aggcgtcgga
      841 agagacgggg gcgccgcagc ccctacctcg ccggatcacg gtggtggggg cgggagtgag
      901 cggaatttcc ctggggcgtt ttgcggcggt tcgcggggtc caaacctttg tcagtgacca
      961 aaaaatactg cgggaagacc agaagaacgc tctggcggaa gcgggcattc cctgggaaga
     1021 agggcacact ccccgggccc ttgagacgga cctactccta ttgagctcag ggatttcccc
     1081 gcaggcgccc ttggtacgag aggcccgaga tcgaggcatc gcgataaagg gagagttgag
     1141 cttcctgggg ccctaccttc gaggccgtct ggtgggcgtc acggggagca acgggaaaag
     1201 taccacggtt acgctcctgg ggcatatgct tcagcgggag gggcgagccg tggcggtggc
     1261 ggggaatgtg ggcacgcccc tgggggatgt ggctgaccgc ccctatgaca tcatcgtcat
     1321 ggagttgagc agctttcaac tccactggag cgagggacta cccctggagt gcggcgccat
     1381 tactaacctg gatccggacc atatcgactg gcatgggtct tatgaaagct atgtcgcgga
     1441 taaaatgaat attttccgag ggctctctcc ctcgggggcc atcgtggccc agtcccggga
     1501 gtgggcgtcc cttccggaag gcgagcgcca ccgggtcctt cttctggggg aagagggcga
     1561 tccccgggct tccttggtct tgtctccccg gggggccatc cttcgtcggg ggcatggatc
     1621 caccgcgctt ttttccagag aagacctgcc ccttctgggg cgccacaacg aagagaacgc
     1681 cgccatggcc atggcactgg cggtgctttt gggggaggat ccagaggtcc ttaggccccg
     1741 gatggcgacc tatagccccc ttccccaccg gtgtgccttt gtggcccgat gggaaggggt
     1801 gacttttgtc gacgactcca aaggaaccaa cgtggccgcc agcgtgacgg cgcttcggtc
     1861 actggagggg cccaaggtgg ttatcctggg ggggcggggc aagggggaaa gttacgactc
     1921 tctggccagg gcggtgaagg agtgcgcccg ttgggcgttg gtcatgggag aggagcgtcc
     1981 gaggctcttg gaagcccttc gccggggaga agtggaggga gtgaaagagg tctccggcat
     2041 ggaagaggct gtccaggagg ccttttcccg ggcccttccc
//
LOCUS       DAOOER010000174         2034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_54894_length_2034_cov_6.156139, whole genome shotgun sequence.
ACCESSION   DAOOER010000174 DAOOER010000000
VERSION     DAOOER010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54894_length_2034_cov_6.156139"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(389..1318)
                     /locus_tag="PL162_10325"
     CDS             complement(389..1318)
                     /locus_tag="PL162_10325"
                     /inference="COORDINATES: protein motif:HMM:NF015724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HQF92454.1"
                     /translation="MILERRKNPGVRRFWILLGVWLVAALPWSGAEARMRLAVVDFQD
                     NASGAGVRMGEPIRKAITDMLTTELFKTQLFTLVERTRIEAIAREHRLSAAGLVAPEQ
                     AVQLGRLLGAGAIITGAITQFALQEFATPGNSDRAGYVTLDVRVIDAESGEVLLVAQG
                     QGKADKIKGADYFRDVTQGEQEGQGILGAATMDAVRRVVGQLVASPHLVEAFHLIDGG
                     GPRVKIDAGSTRGGVKRGDLFVVYVEGEPVLGVDGSVLGVDRTMLGVLRVTAVQPRFS
                     TAELVRGEASQLRRGDFVMRLEGAPEDVELGSR"
     gene            complement(1315..1914)
                     /locus_tag="PL162_10330"
     CDS             complement(1315..1914)
                     /locus_tag="PL162_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92455.1"
                     /translation="MKTRPKYVAVTVAVVERWDETPLWSASAPLDVLGGTPVGSTTAA
                     SSSAASSSATVASAPSAVSSSPSSAAPLPTEASGQAASAPTPELSGGSPENDFRNTSE
                     EMAVIDAYPIDEALKKDLKMLHKGGYFSYSAKNYAQAYQAFSRALELYDGNYLDAYWA
                     ARAAHKAGQKNAMKLFLEKALTINPQYRPAKQYQEAYGK"
     gene            complement(1916..>2034)
                     /locus_tag="PL162_10335"
     CDS             complement(1916..>2034)
                     /locus_tag="PL162_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HQF92456.1"
                     /translation="EVFFNRLKHFRRVATRFEKLADSFLAMVHMVCWMLWLR"
BASE COUNT          382 a          700 c          589 g          363 t
ORIGIN      
        1 aacaagagac ccttttcaac gcccttgtcc acgagtggct ccaccgcatg gaggaacatg
       61 cccccaaggc cgacagagcg gcgccgtagc ttcgggcgtc ggcggagggg cgggcttccg
      121 gctctcttcc ggccatgccc cgggacggga aaaaaggcta cggacacttt tcggaaaaga
      181 ggtcggcccc ccgctttaag attttcttta ataaattcct ggcggtgcca gggcatcttt
      241 ggcacatccc gagggctttc tgcaaacttt ttcgtcgggg gaggcgcgac atctctcccg
      301 acacatgggg aaagccccgg agggcgtccg atttcaggcg cactccgggg cttcttgcac
      361 gagctgacga caagccgtct ctgttcgcct accgcgatcc gagctccacg tcctcggggg
      421 ccccctccag gcgcatgacg aaatcccccc ggcgaagctg ggaagcctcc ccccgcacca
      481 gctctgcggt ggaaaagcgg ggctgcaccg ccgtgacccg cagcacccca agcatggtgc
      541 gatccacacc gaggacgctg ccgtccactc ccaacaccgg ctccccctcc acgtagacca
      601 cgaagaggtc accccgcttg acgcctcccc gggtggatcc cgcatcaatc ttcacccggg
      661 ggccccctcc atcgatgagg tgaaaggcct ccaccaaatg gggcgatgcc accagctgcc
      721 ccaccacccg gcgcaccgcg tccatggtgg cggctcccag gatgccctgt ccctcctgct
      781 cgccctgggt cacgtcgcga aaataatccg cccccttgat cttgtccgcc ttgccctgtc
      841 cctgggccac cagaagcacc tcgccgctct ccgcatcgat gacccgcacg tcgagggtca
      901 cgtaccccgc gcggtcgctg tttcccgggg tagcaaactc ttgtagcgca aactgcgtga
      961 tggcgccggt gatgatggcc cccgccccca gaaggcgacc gagctgcacc gcctgctccg
     1021 gcgccacaag gcctgcggcg gaaagacggt gttcccgggc gatggcctca atgcgcgtgc
     1081 gttccaccag ggtaaagagc tgggtcttga agagctccgt ggtcagcatg tccgtgatgg
     1141 ccttgcgaat gggctccccc atccggaccc ccgcgccgct ggcgttgtcc tgaaaatcta
     1201 ccacagccag ccgcatccgg gcctccgccc cgctccaggg cagggccgcc acgagccaca
     1261 cccccagaag gatccaaaac cgccgcactc cggggttctt tctcctttcc aggatcattt
     1321 cccgtaggcc tcctgatact gcttggcggg cctatactgc gggttgatgg tcaacgcctt
     1381 ttctaggaaa agtttcatgg cgttcttttg ccccgccttg tgcgccgccc gggcggccca
     1441 gtaggcgtcg aggtaattcc catcgtagag ctccagagcc cgggagaagg cctgatacgc
     1501 ctgggcgtag ttttttgcgg aataggagaa ataccccccc ttgtgcagca tcttcagatc
     1561 tttcttcagc gcttcatcaa tgggataggc gtcgatgacc gccatttcct ccgaggtatt
     1621 ccgaaagtcg ttctccggcg atccaccgga aagctcgggg gtcggagcgg aggcagcctg
     1681 tccggaggcc tccgtcggca aaggcgcggc agaagatggg ctcgacgaca ccgccgatgg
     1741 agcggaggca acagtggcgc tcgaagacgc cgccgacgaa gacgcggcgg tggtcgatcc
     1801 cacaggggtg cctcccagaa cgtccagggg tgcggaggcg ctccagaggg gcgtctcatc
     1861 ccagcgctcc accacggcca ccgtcaccgc cacgtactta ggtcgtgttt tcaaatcacc
     1921 gcaaccagag catccagcag accatatgaa ccatggccag aaaactgtct gcaagctttt
     1981 cgaagcgggt ggcaactctt cggaaatgct taagacgatt gaaaaacact tcca
//
LOCUS       DAOOER010000175         1999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_56431_length_1999_cov_8.294239, whole genome shotgun sequence.
ACCESSION   DAOOER010000175 DAOOER010000000
VERSION     DAOOER010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56431_length_1999_cov_8.294239"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1279
                     /locus_tag="PL162_10340"
     CDS             <1..1279
                     /locus_tag="PL162_10340"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQF92457.1"
                     /translation="DRLALSSSGDRRQDVLKQYRAAMDHLIPLSDAMGETVVSLVENN
                     NAVTAQLVEDDLAMAEWIKSISAVFTGVGLAMTILLGWAITRGISVPIGVGVSLMARM
                     SRGDLTRDVPPEFLSRGDEIGDLARAVQELTEDLRLQVSALKDATAVLGATSSQIAAS
                     AAQITAGAEETAVAVVETTASVEEIRATAEATTRKTREVAEHTQQGLHLVQGGRKATE
                     DLTEGIQRISTQMAFIAETIVKLSEQSQEIGEITGTVEELAEQSNLLAVNAAVEAARA
                     GDQGRGFSVVAQEIKSLAEQSKEAAKRVQRILKDVQKATAAAVMATEQGSKAVDQARA
                     SAPSRESLQGMSRSFAESAQSAAQIAAANAELLSGVDQAAQAMTSIREAGHQNVEGMR
                     EMENAAQSLKDMGQRIADLVGRYALKGDEERDG"
BASE COUNT          435 a          585 c          646 g          333 t
ORIGIN      
        1 ggatcgtcta gcgctctcct cctcgggaga tcgtcgccag gatgtcttaa agcagtaccg
       61 ggccgccatg gatcacctca tccccctttc cgacgccatg ggagagaccg tggtgtccct
      121 ggtggaaaat aataacgccg tgacggcgca gctggtcgaa gacgacctgg ccatggcgga
      181 atggattaaa agtatcagtg cggtctttac cggcgtggga ttggcgatga cgatccttct
      241 gggatgggcc atcacccggg gcatcagcgt tcccatcggc gtaggggtct cccttatggc
      301 gcgcatgagc cggggcgacc tcacccggga cgttcctccg gagtttcttt cccgggggga
      361 cgagatcggc gacctggccc gggctgttca ggaactcacc gaagacctgc gattgcaggt
      421 gtccgcccta aaggacgcga cggcggtcct gggggcgacg tcgagtcaga tcgccgcctc
      481 ggcggcccag atcaccgccg gggccgaaga gacggcggtg gcggtggtgg aaaccacggc
      541 aagcgtggaa gagatccggg ccacggcgga ggccacgacc cgcaagaccc gggaggtggc
      601 ggaacacacc caacaggggc tccatctggt ccaaggggga cgcaaggcca cggaggacct
      661 gacggagggc atccagcgca tcagcaccca gatggctttc atcgcggaaa ccatcgtcaa
      721 gctcagcgag cagagccagg agatcggaga gatcactggc acggtggagg agctggcgga
      781 gcaatccaac ctgctggcgg tgaacgccgc cgtggaggcc gcccgggccg gggatcaggg
      841 gcggggattt tccgtggtgg cccaggagat aaagagcctg gcggagcaat ccaaggaagc
      901 ggccaagcgg gttcagcgca tattgaaaga tgtgcagaaa gccactgcgg cggcggtcat
      961 ggccacagag cagggctcca aggccgtgga tcaggcgagg gcctcggccc cctctcggga
     1021 gtctctccag ggcatgagcc ggagctttgc cgaatccgcc caatccgccg cccagatcgc
     1081 cgccgccaat gccgagctcc tgtcgggggt ggaccaggcg gcccaggcca tgaccagcat
     1141 tcgcgaggcg ggccaccaga acgtggaagg catgagggag atggaaaacg ccgcccagag
     1201 cttaaaagac atggggcagc ggatcgccga tctggtgggg cgttatgcgc tgaagggcga
     1261 tgaggagagg gatggatagg caacacgggg cgaaagatcc ccgacagaag acgatggccc
     1321 tttgcccggg gcccgaaccg gggggaaagc cccggcggat cgccgacggg aaaacgccac
     1381 cccaggggga ataaccgggg ataaccatgg gatcgggcac accggaggga tctgaggcaa
     1441 catacgtcgg caattttgac agaaaatagt ctttctttta aattatccaa agtcatcaag
     1501 gtaaagcgcg gcctccccgg actcccttgc atggcattca gtgctatcat tgcgggtcca
     1561 cggagcccct ctcgatttag gacgtcagga tcgatggagg ccgcatcaat ttttccgcga
     1621 atcccccctt agtcccatca ctagaactcg aaggttcttc ccgcaggcag aacgcgcctt
     1681 ggatctcccc cagataggtc tcttcgcaac cctggatgct atcgccgcac aagtgatcga
     1741 atgacgcttt gatctggtca tgatttgcat aaaaaaagtc acagtcgccg aactcccctc
     1801 cgcaccgcgc ccgattctaa aaccaagata tcgagaaata aggcgacccg ggacgattac
     1861 gcccccgatc cgaaggaccg ggctcccatg ccttgacaat ttcccccggg gagcgtattc
     1921 tttggttgtt agtcgagtct aactttctgg aggtttccat gggcgtctca tcccgcaccg
     1981 aagactatct tgaggagat
//
LOCUS       DAOOER010000176         1971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_57604_length_1971_cov_6.445720, whole genome shotgun sequence.
ACCESSION   DAOOER010000176 DAOOER010000000
VERSION     DAOOER010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1971
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57604_length_1971_cov_6.445720"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="PL162_10345"
     CDS             complement(<1..995)
                     /locus_tag="PL162_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008522999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HQF92458.1"
                     /translation="MTIDMPFVEKFLLELLAIPSVPGDCEAAAERVRKEFDALGLSWR
                     TTPKGAVVGVWPGQEAGDERLVSAHVDTLGAVVRQIKSTGRLKLLPIGGFAWGAMEGE
                     NLLVRTADGREFSGSLLPEKASIHAFSETVREAPRNDDTVEVRLDAPVSCKEDVEKLG
                     IAPGDFVFFDPRPVITGEGFIKARFLDDKASVACLFGALRAMKAQGLSPRKTTHFYIS
                     NHEEIGHGVARFPDAVTEFVALDIGIVAEGTESSEHCVTIAARDSRSPYHLPLRRRLT
                     SLARERGIDFRTDVFYRYGSDATIAVLQGADVAFACFGPGVDATHHYERTHRKAL"
     gene            complement(992..1957)
                     /locus_tag="PL162_10350"
     CDS             complement(992..1957)
                     /locus_tag="PL162_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008521404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQF92459.1"
                     /translation="MVDVRHLKKYFKTPRGMLHAVDDVSFSIARGETLGLVGESGCGK
                     STLGRAMIRLLDVTGGEVWFDGDDILTYSEASMKALRKRVQIVFQDPYSCLNPRLSVA
                     ELIAEPLIVNKIFRTRPEMNARIKELMDTVGLAERLFGAYPHELDGGRRQRIGIARSL
                     ALEPEFIVLDEPVSALDVCIQAQILNLLNNLKKEFGYTYVFISHNLSVVKHVSDRIAV
                     MYLGKMVELAGSRTIFQDPLHPYTQALLSAIPLPKMDVVRDRIILEGDVPSPIEPPEG
                     CRFAGRCRYRQELCRTKTPELRDIDPGHAVACHFARNLSQGEASK"
BASE COUNT          370 a          631 c          617 g          353 t
ORIGIN      
        1 tccagggctt tccggtgggt ccgctcgtag tggtgggtgg cgtccactcc ggggccaaag
       61 caggcaaagg ccacgtcggc cccctggaga acggcgatgg tggcatcgga gccataacga
      121 tagaagacgt cggtgcgaaa atctatgccc cgctcccggg ccagggacgt cagccggcgg
      181 cgcaggggca gatggtacgg ggatcgactg tcccgggcgg cgatggtgac gcagtgctcg
      241 gaagactccg tcccttcggc cacgatgccg atgtccagcg ccacgaactc cgtcacggca
      301 tcgggaaaac gggctacccc atggcctatt tcttcgtggt tgctgatgta aaagtgcgtg
      361 gtcttccgag gagagagccc ctgggccttc atggccctca gggcgccgaa gaggcaagcc
      421 accgaggcct tgtcgtccag gaatcgggcc ttgatgaacc cttcccccgt gatcaccggc
      481 cgggggtcga agaacacgaa atctcccggg gcgatgccca atttctccac atcctccttg
      541 caggagaccg gggcatccag gcgcacctcc acggtgtcgt cgttccgggg agcctcccgc
      601 acggtttcgg aaaaggcatg gatcgaggcc ttttccggca gaagagatcc ggagaactcc
      661 cggccgtcgg cggtgcgcac cagaaggttt tctccctcca tggcccccca ggcaaatccc
      721 ccgatgggga ggagcttcag ccgcccggtg cttttgatct gccgcaccac ggcgcccaac
      781 gtgtccacgt gggccgagac cagccgctcg tcccccgctt cctgccccgg ccacaccccc
      841 accaccgccc ccttgggggt ggtgcgccac gagaggccca gggcgtcaaa ttccttacgg
      901 acacgctcgg ccgccgcctc acagtctccg gggaccgagg gaatggccag gagctccagc
      961 agaaactttt ccacaaaagg catgtctatg gtcatttgct cgcctctcct tgagaaagat
     1021 tccgggcaaa gtggcacgcc accgcgtgcc ccggatcgat gtcccgaagc tccggggtct
     1081 tggtgcggca cagttcctgg cgatagcggc accgcccggc gaaacgacac ccctcggggg
     1141 gctctatggg gctgggcaca tccccttcga ggatgatgcg gtcccgcacc acgtccatct
     1201 tgggcagggg aatggcggaa agcagggcct gggtgtaggg gtgcagggga tcctgaaaga
     1261 tcgtccggct tccggcgagc tccaccatct tgcccagata catgacggcg atgcggtccg
     1321 acacgtgctt caccacgctc aggttatggg agataaacac gtaggtatac ccaaattctt
     1381 ttttgagatt attcaacaga ttcaggatct gggcctgaat gcagacgtcc agggcggaaa
     1441 cgggttcgtc cagcacgata aattcgggct ccagcgccag ggatcgggca atgccgatgc
     1501 gctggcgccg ccctccgtcg agctcgtggg ggtaggcccc gaagagtctt tccgccagcc
     1561 ccacggtgtc catgagctcc ttgatgcggg cgttcatctc cggtcgggtg cggaagatct
     1621 tgttcacgat gaggggttcg gcgatgagct ccgccaccga aaggcgcggg ttcaggcagg
     1681 aatagggatc ctggaagacg atctgcaccc gcttccgcag ggccttcatg gacgcctcgg
     1741 agtaagtcaa gatatcatcg ccgtcaaacc acacttcgcc gccggtgaca tctagaaggc
     1801 ggatcatggc tcgccccagg gtggacttac cgcaccccga ctcccccacg agccccagag
     1861 tttccccccg ggcgatggaa aagctcacgt cgtccacggc gtggagcatg cccctggggg
     1921 tcttgaagta ctttttcagg tgcctgacat ctaccaaagg ccgtccgttc a
//
LOCUS       DAOOER010000177         1954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_58397_length_1954_cov_5.474460, whole genome shotgun sequence.
ACCESSION   DAOOER010000177 DAOOER010000000
VERSION     DAOOER010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58397_length_1954_cov_5.474460"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1177)
                     /locus_tag="PL162_10355"
     CDS             complement(<1..1177)
                     /locus_tag="PL162_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQF92460.1"
                     /translation="MRRITGWKVALMVGMVVCLGVSGAFAEDKIIKIGTLFPLTGSVA
                     TAGQRCQAAVETAVEVVNGSHPELQVPLAAQEGILGGYKIVLVNADTQGKPDVAKAEA
                     ERLYNQEGVYAVIGCYNSSSTKPASAVAERMKKIFMCGCSSSAALTERDFSYFFRLAP
                     TDKTESAEFVDVMKWLNDSKQAGVQSVGLIYENTEFGKHAAQEGKAAAEAAGFTVVAD
                     VPFSPGATNMDSEVQTLKAANPDVVFGACLGGDYTLWVNTMKKMAWLPKMTLNYCTGY
                     QDPLIAKQLGPDGDFFMGGTGYSPEFAAMMPAVAAVEKIYTEKTGVPFDSDSIQEAVA
                     LFVLAQAIEKAGTLDYAKVQETLMANEWEAPLTLAGKVAFVSGGQNVQAKSMITQLQG
                     "
     gene            complement(1440..>1954)
                     /locus_tag="PL162_10360"
     CDS             complement(1440..>1954)
                     /locus_tag="PL162_10360"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQF92461.1"
                     /translation="DATGAPGCISTAVTVGATTKSDVVATYSNSASFVDVLAPGSSIN
                     SSIPGGGFDSWNGTSMAAPHVAGAFAVLRQAYPTLTVDQIEDALKSTGLPVSDSPRGA
                     AAPLAITKPRIRVKEAFDALAAVTPTPTGIPTVPPITGGGGGSGGGCTAGAAPWALLL
                     ALPLMALLRR"
BASE COUNT          329 a          656 c          598 g          371 t
ORIGIN      
        1 ccccctggag ctgggtgatc atgctcttgg cctggacgtt ctgccctccg gagacgaaag
       61 ccaccttccc cgccagggtc aggggcgctt cccactcgtt ggccatgagg gtttcctgga
      121 ccttggcgta gtccagcgtc cccgcctttt cgatggcctg ggccagcacg aagagagcca
      181 cggcctcctg aatgctgtcg ctatcgaagg gcacgccggt tttttccgtg taaattttct
      241 ccaccgccgc caccgcaggc atcatggcgg caaattccgg ggaatatccc gtgccgccca
      301 tgaagaagtc cccgtcgggg cccagctgct tggcgatcag gggatcctgg tatcccgtgc
      361 agtagttcag ggtcattttg gggagccacg ccatcttttt catggtattc acccacaggg
      421 tgtagtcccc tcccaggcag gcgccaaaga ccacatcggg attggcggcc ttgagggtct
      481 gcacttcgct gtccatgttg gtcgccccgg ggctgaaggg cacatcggcc accacggtaa
      541 atccggcggc ctctgccgcc gccttgcctt cttgggccgc gtgcttgccg aactcggtat
      601 tctcatagat gagccccacg ctctggaccc ccgcttgctt gctgtcgttg agccacttca
      661 tgacatccac gaactccgcc gactcggtct tgtccgtggg agccaggcgg aagaagtagc
      721 tgaagtccct ctcggtgagg gcggcggagc tggagcagcc gcacatgaag atcttcttca
      781 tgcgctcggc cacggcgctg gcgggcttgg tggaggagct gttgtagcac ccgatgacgg
      841 cgtagacccc ctcttggttg tagaggcgct cggcttcggc cttggccacg tcgggtttcc
      901 cctgggtgtc cgcattcacc aacacgatct tatatccccc caggatccct tcctgggccg
      961 ccaggggaac ctggagctcc ggatggctac cgttgaccac ctccaccgcc gtttccacgg
     1021 cggcttgaca gcgctgcccc gccgtggcca cggaccccgt caggggaaag agggtgccga
     1081 tcttgatgat cttatcctcg gcaaaggccc cactcactcc cagacaaacc accatgccca
     1141 ccatcagtgc taccttccat cccgtgatgc gccgcatgct tcttcctccc ttctcgctct
     1201 ctttttcaag agaccccggg gctcgccccg tcgaaggtct cccgggcccc gatttccggg
     1261 aaaccttcgc gccgcattct cacggcgcat cgcgccatta ccatcatagt atggagcaaa
     1321 ctaccgcttc gtcaagggga gagattcctt aaggtccttc caaaggaaaa aaaagacccc
     1381 ggggagagct ctccatggag agccctcccc gggcctaggc gttacaaaac ctagggacgc
     1441 taccggcgaa ggagcgccat gaggggcagg gccaggagaa gggcccaggg ggccgccccc
     1501 gccgtgcaac cgccgccact cccgccaccc ccgccggtga tggggggcac ggtgggaatc
     1561 ccagtgggcg taggcgtcac ggctgccagg gcgtcaaagg cttccttcac ccggatccgg
     1621 ggcttggtga tggccaaagg agccgccgcc ccccggggtg aatcgctgac gggaaggccc
     1681 gtgctcttca gggcgtcttc gatctggtcc accgtgaggg tcggataggc ctgacggagc
     1741 accgcgaagg cccccgccac gtggggcgcc gccatggagg tgccgttcca gctgtcaaat
     1801 cctccccccg gaatggaaga gttgatggaa gaccccgggg ccagcacgtc cacgaaggat
     1861 gcgctgttgg agtaggtggc caccacgtcg ctcttggtgg tggctcccac ggtgaccgcc
     1921 gtggagatgc accccggagc ccccgtggcg tcgg
//
LOCUS       DAOOER010000178         1940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_59036_length_1940_cov_5.208488, whole genome shotgun sequence.
ACCESSION   DAOOER010000178 DAOOER010000000
VERSION     DAOOER010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59036_length_1940_cov_5.208488"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /gene="argH"
                     /locus_tag="PL162_10365"
     CDS             complement(<1..1007)
                     /gene="argH"
                     /locus_tag="PL162_10365"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HQF92462.1"
                     /translation="MWKGRFSEDPDARVQAFTQSLDLDWRLAAADLRGSVAHVRMLGA
                     VGLLSPEEARTLEEHLEGVGEEIRTGVLAPRAELEDVHMTIEARLTERCGPLGARIHM
                     GRSRNDQVATALRLYLRGAILELQDALDDLLSVLLGRAQSHAEVLVPGYTHLQQAQPV
                     ALGHYWMAHFWAFLRDDKRFSAAHDAADECPLGSGALAGSTLPLDRTFVARDLGFSRV
                     TDNSMDSVASRDYLLDALYAAASFGIHASRLAEDLIIYASTEFGWIRLPDAFCTGSSM
                     MPQKKNPDVLELIRGKAGQFVGHLVDLMTTTKGLPLTYNRDLQEDKRSLWGALDTAAA
                     VL"
     gene            complement(1007..1705)
                     /locus_tag="PL162_10370"
     CDS             complement(1007..1705)
                     /locus_tag="PL162_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92463.1"
                     /translation="MNLDAREHVLVGRIWSGVLGGVVLFLLFPPTRELFRQLTAAHPY
                     LLGVLKIGALGAMGDMLAVRMRGGRWRYQNIGLRDKALVWGFLGLVFVVVFPIFSVGT
                     DFLFRQGYLSNPFEGFGARALEAFAASSFMNLLFAPTMMVFHRITDMAIAEGGIFRPW
                     SAVEMLRRMDWNSMGRIVFPSLLWFWIPAHTLTFILPPEFRIVSAALLAIALGFILSF
                     ASRSSGTPGRKEGR"
     gene            complement(1720..>1940)
                     /locus_tag="PL162_10375"
     CDS             complement(1720..>1940)
                     /locus_tag="PL162_10375"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009201870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQF92464.1"
                     /translation="GGGVISFYMHLSDIAVREGDVVKRGQMVGKSGSTGRSTGPHLHL
                     GLCLQGRLVDPSSLFGPEKTSGETKKKK"
BASE COUNT          375 a          648 c          624 g          293 t
ORIGIN      
        1 agcacggcgg cggcggtatc cagggcgccc caaaggctcc gcttgtcctc ctggaggtct
       61 cggttgtagg taaggggcaa acccttggtg gtggtcatga ggtccaccag gtgccccacg
      121 aactgccctg ccttgccccg gatgagctcc aacacgtcgg ggtttttctt ctggggcatc
      181 atgctcgacc ccgtgcaaaa ggcgtcgggg aggcggatcc agccgaactc cgtggaggcg
      241 tagatgatca ggtcctccgc caggcggctg gcgtggatcc cgaaggatgc agcggcgtag
      301 agggcatcca agaggtagtc ccgggaagcc acgctgtcca tgctgttgtc cgtcacccgg
      361 gaaaagccca gatcccgggc cacgaaggtc cggtccaggg gcagggtgga gcccgccagg
      421 gcccccgagc ccagggggca ctcgtcggcg gcgtcatggg cggcggaaaa gcgcttgtcg
      481 tcccgcaaaa aggcccagaa atgggccatc cagtagtgcc ccagggccac gggctgggcc
      541 tgctgcaagt gggtataccc cggcaccagg acctcggcgt ggctctgggc ccggcccagc
      601 aggacggaga gcaggtcgtc cagggcgtct tgcaactcca agatcgcgcc ccttaggtaa
      661 agtctcaggg cggtggccac ttggtcgttt cggcttcgcc ccatgtggat ccgggccccc
      721 agggggccgc accgttccgt gagccgggcc tcgatggtca tgtgcacgtc ttccagctcg
      781 gcccggggcg ccaggacccc ggtgcggatc tcctccccca cgccctccag gtgttcctcc
      841 agcgtccggg cctcctcggg cgaaaggaga cccacggcgc cgagcatgcg gacgtgggcc
      901 acgctccccc ggaggtccgc cgccgccagg cgccaatcaa ggtccagcga ctgggtaaag
      961 gcctgcaccc gggcgtcggg gtcctcggaa aaacgcccct tccacatcat cgcccctctt
     1021 tacgccccgg ggtcccggag ctccgggaag cgaaggagag gatgaaaccc agggcgatgg
     1081 ccaacagcgc cgcagaaacg atgcggaact ccggcggcaa aatgaacgtc agggtgtggg
     1141 cgggaatcca gaaccagagc agcgagggga aaacgatgcg ccccatggag ttccagtcca
     1201 tcctccggag catctccacg gcactccagg ggcggaagat gcccccttcg gcgatggcca
     1261 tgtcggtgat ccggtgaaag accatcatgg tgggggcaaa aagcaggttc atgaaggagg
     1321 atgcggcgaa ggcctccagg gcccgggccc cgaaaccctc gaaggggttg gacagatacc
     1381 cctggcgaaa gagaaagtcc gtccccacgg aaaagatggg aaagaccacc acgaagacca
     1441 gaccgagaaa gccccagacc agggccttgt cccggaggcc gatgttctgg taacgccacc
     1501 gccctccccg catgcgcacc gccagcatgt cccccatggc gcccaaggcg ccgatcttca
     1561 gcacccccag cagataggga tgggctgcgg tgagctggcg aaagagctcc cgggtgggag
     1621 gaaagagcaa aaagagcacc acgcccccca gcacgccgct ccagattctt cccacaagca
     1681 catgttcccg cgcatccaga ttcaacgttc cgcctcctct cacttctttt tctttgtctc
     1741 gccggacgtt ttctccggac cgaaaaggga cgaggggtcc accagtcgtc cctggagaca
     1801 gagccccaga tggaggtggg gccccgtgga gcgccccgtg gaacccgatt ttcccaccat
     1861 ctgtcccctt ttcaccacgt ccccttcgcg cacggcgatg tccgacaggt gcatgtagaa
     1921 ggagatcacc cctcctccgt
//
LOCUS       DAOOER010000179         1924 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_59778_length_1924_cov_5.960407, whole genome shotgun sequence.
ACCESSION   DAOOER010000179 DAOOER010000000
VERSION     DAOOER010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59778_length_1924_cov_5.960407"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="PL162_10380"
     CDS             complement(<1..544)
                     /locus_tag="PL162_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HQF92465.1"
                     /translation="MNPKRQRMQFSAWYMVGALFMVWLFNDLVFRPMLLSETEVPYST
                     FLQNLTSGDVKGVTVTTDRVIYEVRSDGGKPHVRNAVRVEDGELVSRLLGARVPFEAQ
                     NQTRSLLEVLLGWVLPFLPLLFIWYIFYRRMGEGGGMSFMSVGKSKAQEIHGEITGIT
                     FSDVGGVGEAEVELREIIEFL"
     gene            complement(794..>1924)
                     /locus_tag="PL162_10385"
     CDS             complement(794..>1924)
                     /locus_tag="PL162_10385"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQF92466.1"
                     /translation="EGMVSVSPDALERNTTPPPVYLSALRRYGVPLSLDRAPERLQEL
                     TLRWPENSFEFDFVALNYLFPEKNRYAYKLEGLDDRWFDAGDRGTGRYVALPGGTYVL
                     RMKASNNDGVWSPEGASLRVTVVPPFWERDSFALAVGGAFLGGIALLWFWRIRQVRAR
                     ELDLATKVHRRTAQLAAANDRLQREIADRRRAEEALRRALVTDELTGLLNRRGFYERA
                     QEMEAQARELSRALFLVYMDMDNFKEINDGFGHGVGDEALREMASLISGVFGDGTIRA
                     RLGGDEFAVLGMADDRDHFGPHLVGHFRNVLEERNALTPRPYRLEPSLGTALGSGDLG
                     EVELLVRRADDAMYEEKMARRLRRLKRDRPGGSFSAPAAPEG"
BASE COUNT          313 a          681 c          590 g          340 t
ORIGIN      
        1 tgaggaattc gatgatctcc cggagctcca cctccgcctc ccccacccct cccacatcgg
       61 agaaggtgat ccccgtgatc tctccgtgga tctcctgggc cttgctcttg cccacggaca
      121 tgaagctcat cccgcccccc tcgcccatgc gccggtagaa gatgtaccag atgaaaagca
      181 gaggaaggaa ggggaggacc caccccagga gcacttccag gaggctgcgg gtctggtttt
      241 gggcctcgaa gggcacccga gcccccagga gccgggatac gagctcccca tcttccacgc
      301 gcacggcgtt gcgcacgtgg ggcttgcccc cgtcgcttcg cacctcgtag atgacccggt
      361 cggtggtgac ggtaaccccc ttcacgtcgc cgctggtcag gttttgcagg aacgtgctgt
      421 agggcacctc ggtctcgctc aggagcatgg ggcgaaagac taggtcgtta aagagccaca
      481 ccatgaagag ggcccccacc atgtaccagg cggaaaactg cattcgctgc cttttgggat
      541 tcaccggccc tcctccctgc ggggagagga tgggcctttt tctcgggtcc ttacgccgcc
      601 cccaccggga cggcggggtt ccccccgtaa aggtcttctt ctttgccgcc ccttcgggcc
      661 tgggatcctc ctgggtcatc cgcatcgtcc ctttctaaac aggcgcccct cggggggcct
      721 ttttttcggg cgaggcccct cgggccccgg ggcttacgcc ctagattttt tgccctcccc
      781 ggcggggaag ggcctaaccc tccggagccg ccggcgcgga aaaactgccc ccggggcggt
      841 cccttttgag ccttctcagg cgccgggcca tcttttcctc gtacatggcg tcgtcggccc
      901 ggcgcaccag gagctccacc tctcccagat cccccgatcc cagggccgtc cccaggctcg
      961 gttccaggcg gtagggacga ggcgtaaggg cgtttcgctc ctccaggacg ttccggaagt
     1021 gccccacgag gtggggacca aagtgatccc ggtcgtcggc catccctaag acggcgaact
     1081 cgtccccccc cagccgggcc cgaatcgtcc cgtcgccaaa gaccccgctg atcagcgagg
     1141 ccatttcccg cagggcctca tcccccacgc cgtggccaaa accgtcgttt atttctttga
     1201 aattatccat gtccatatag accaaaaaga gggctcggga aagctcccgg gcctgggcct
     1261 ccatctcctg ggccctctcg tagaagcccc tccggttcag gagccccgtg agttcatccg
     1321 tcaccaacgc gcgccgcagg gcctcctcgg cccggcggcg gtcggcgatc tccctctgga
     1381 ggcggtcgtt ggcggcggcg agctgggccg tcctccggtg caccttcgtg gcgaggtcga
     1441 gctcccgggc ccggacctgg cggatcctcc aaaaccacag gagggcgatc cccccgagga
     1501 aggcccctcc gacggccagg gcgaaggaat cgcgctccca gaaggggggc accactgtca
     1561 ccctcaagga ggccccctcg gggctccaga ccccgtcgtt attcgacgcc ttcatgcgca
     1621 gcacataggt ccctcccgga agggccacgt agcgccccgt gccccggtct cccgcgtcaa
     1681 accatcggtc gtccagcccc tcgagcttgt aggcgtagcg gtttttctcg ggaaagaggt
     1741 agttgagggc cacaaaatcg aactcgaagg agttctccgg ccaccggagg gtcagctcct
     1801 ggagcctttc gggggcccgg tccagcgaaa ggggcacccc gtagcggcgg agggcggaaa
     1861 ggtacaccgg gggcggggtg gtgtttctct ccagggcgtc gggggaaacg ctcaccatgc
     1921 cctc
//
LOCUS       DAOOER010000180         1912 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_60385_length_1912_cov_6.779214, whole genome shotgun sequence.
ACCESSION   DAOOER010000180 DAOOER010000000
VERSION     DAOOER010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60385_length_1912_cov_6.779214"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(187..696)
                     /locus_tag="PL162_10390"
     CDS             complement(187..696)
                     /locus_tag="PL162_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92467.1"
                     /translation="MQIFSVDGLCFDFPDDWLVTKYDDWAFYRKQFVRMWDGIKAVDL
                     LALSPEGTLYLIEAKDYRRHRREKGLGIADEAAHKAFDTLAALLPAKIRAPDGPERDV
                     AMRFLSAVKLQVVLHLEQPQTQSRLFPRIDPANVLQKLRQLVKPLDAHPVVAGMGRMG
                     SLAGKWAVR"
     gene            complement(708..1748)
                     /locus_tag="PL162_10395"
     CDS             complement(708..1748)
                     /locus_tag="PL162_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005658876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQF92468.1"
                     /translation="MLRRMHIRNMTVFSDADLIFSPALNVFAGENGTGKTHILKVAYA
                     LLAATAAEGRIPGATPSKTVLQPRIADKLVAVFRAETLGRLARRRQGRARCDVALSFD
                     NERRNTAFSFATNSKSEVSVDQLPGEWGDVAPVYFPTRELLTIYPGFVPMYDAHYLEF
                     EETWRDTCLLLGAPVRKGARERRVRELLEPLEDAMGGSMELDGNGRFYLRTPGGRMEM
                     PLVAEGHRKLGMVARLIATGALLEKGYLFWDEPEANLNPRIVRQVARTILALCANGIQ
                     VFAATHSLFLLRELDILLGNGVRSRFFGLRRGDDGATVEQGDSVDDMGNITALDEELD
                     QSDRYMEQADRA"
BASE COUNT          353 a          594 c          578 g          387 t
ORIGIN      
        1 acccccgacg ccttcctgga gcgtttctcc tacatcaccg acgcggtgta ctgatttttc
       61 ggaggaggcg tgggccctcc ttcggcggaa agaacgtttt tgatacgaac gcgacgaacg
      121 ccctctcccg gttttcctcc ggggaagggc gttcgtgacg tgtggcgccg ctgcctaaag
      181 agcgggctag cgtaccgccc acttccccgc cagagatccc atgcgcccca ttccggcaac
      241 gacagggtgg gcgtccaggg gtttcaccaa ctgccggagt ttctggagca cattggcggg
      301 atcgatgcgc ggaaagagcc tgctctgcgt ctggggctgt tcgagatgca gcaccacctg
      361 gagtttaacc gccgaaagaa atctcattgc cacgtctcgt tcagggccgt cgggcgcacg
      421 gatctttgct ggcagcaggg ccgccagtgt gtcaaaggcc ttatgggccg cttcgtccgc
      481 gatgcccaga cccttttccc gtcggtgtcg gcggtaatct tttgcttcaa tcaggtagag
      541 tgtgccctcc ggggagagcg ccagcagatc caccgccttt atgccgtccc acatcctgac
      601 gaactgcttg cgatagaacg cccagtcgtc atatttcgtc acaagccagt cgtcggggaa
      661 atcaaaacag aggccgtcga cggaaaaaat ctgcacgatt aacctcctta ggcccgatcc
      721 gcctgctcca tgtaacggtc cgactgatcc agctcctcgt ccagggcggt gatgttcccc
      781 atgtcgtcca cggagtctcc ctgttcgact gtcgcgccgt cgtctcctcg acgtaggccg
      841 aaaaatcgcg accttacgcc gtttccgaga aggatgtcca actcccggag cagaaagagg
      901 ctgtgcgtgg cggcgaagac ttggattccg tttgcgcaga gggcaaggat ggtacgggcc
      961 acctgcctga cgatgcgggg gttcaggttc gcctccggtt cgtcccagaa gagatagccc
     1021 ttttccagaa gggctccggt ggcgatcagt cgcgccacca tgcccagttt ccggtggccc
     1081 tcggcgacaa ggggcatctc cattcttccg ccgggggtgc ggaggtagaa ccggccgttt
     1141 ccatcgagct ccatggaccc ccccatggca tcttccaggg gttccaggag ttcccgaaca
     1201 cgccgttccc gggcgccctt tcgcacgggg gccccaagca gcaaacacgt gtcgcgccac
     1261 gtctcttcaa attcgagata gtgggcatcg tacatgggaa caaagcccgg atatatcgtc
     1321 agaagttcgc gcgtcgggaa ataaaccggg gcgacgtccc cccattcgcc gggaagttga
     1381 tctacggaaa cttcggattt gctgttggtg gcaaagctga aggccgtgtt tcgcctttcg
     1441 ttgtcgaagg agagtgccac atcgcatcgc gcccttccct ggcgacgcct cgcaagacgc
     1501 cccagtgtct cggcgcggaa gacggcgact aatttgtccg caatcctcgg ctgaaggacc
     1561 gtcttactgg gagtggcccc agggattcgg ccttccgccg cagtggccgc caggagggca
     1621 taggccactt tgaggatgtg ggttttcccc gtgccgtttt cccccgcaaa gacgttaaga
     1681 gcgggggaaa aaatcaggtc ggcatcggaa aatacggtca tgttccgtat gtgcatgcgt
     1741 cgaagcacga cgaccactct ccctttcctg gacttaggat accggaaggt tgggcccccg
     1801 ggacgcagtg gtcttcgggg gggcttttcg cccctgggcg gggcccccgg gagcggctag
     1861 cagatcctcg gcaggagctc ccccacaggg agatccacca cccgggtgcc tc
//
LOCUS       DAOOER010000181         1906 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_60712_length_1906_cov_9.641815, whole genome shotgun sequence.
ACCESSION   DAOOER010000181 DAOOER010000000
VERSION     DAOOER010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60712_length_1906_cov_9.641815"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..936)
                     /locus_tag="PL162_10400"
     CDS             complement(118..936)
                     /locus_tag="PL162_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92469.1"
                     /translation="MRKKRKILGILLVLVLTLGGKGEGFPLQLDADSLNYDPSMQKVL
                     AQGHVTLRQGESIMTAKTAECRTDGSVFVLRGAVSADLKEEKITLGSEEVLYYAGDKG
                     RLVASGDVILRRGRDAMSCDVVVWRFGPVPSYHARGRVHLAWEEKHLWAKTVSRDGEI
                     FRTSDLERFEDKKQGMTLLAPELEGRFEGDEVVEVVTSGGVRLELRRRDKTVVVTGAK
                     GVYSTKRGTLVVSGGATAKQEGRSVRAESLVLRLDTGRIEALGKPQVVFPISKP"
     gene            complement(1133..>1906)
                     /locus_tag="PL162_10405"
     CDS             complement(1133..>1906)
                     /locus_tag="PL162_10405"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014162745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HQF92470.1"
                     /translation="ITLGRGGSDLSAVALAAALEADVCYIYTDVSGVFTADPRVVPEA
                     RKMARVPFNVCAEMAMLGAKILQARSVEMAMRFNVPVVVLSSFEEEEGTWIMREDISE
                     GLVVQAVVHDRNVAKVAVMGVPDKPGVAAELFSALGQRGLAVEMIIQSVMRGDVNDIA
                     FLVKKEILGEALETCQAFARRVESQGILVDTEVGRVSIVGAGIAHHADVPSRMFSVLG
                     KLGVNIDMIASTSLSITCVVSAHQIEEAVRALHANFIGA"
BASE COUNT          374 a          711 c          470 g          351 t
ORIGIN      
        1 tgggccccag aagccccacg atctctccct ggcgcaccgc aaagcacacc gaagatacca
       61 cggtgcgccc ccggtatccc ttgaccagtc cctgggcaga aaggatccgg gatgccatca
      121 gggcttactg atgggaaaaa ccacttgggg cttccccaga gcctcaatgc gccccgtgtc
      181 caagcgcaac acgaggcttt ccgcccgcac cgaccgccct tcctgtttgg ccgtagcgcc
      241 accggacacc actaaagtgc ctcgcttggt ggagtagacc cccttggccc cggtcaccac
      301 caccgttttg tcgcggcggc gtagctccag acgcactcct ccggaggtca ccacctccac
      361 tacctcatcc ccttcgaagc gcccctccag ctccggggcc aggagcgtca tcccctgctt
      421 tttgtcctcg aagcgctcca ggtcgctggt acgaaagatc tccccgtccc ggctgaccgt
      481 cttcgcccaa aggtgttttt cttcccaagc caaatggact cgtccccggg cgtgatagga
      541 tggcacgggg ccaaatcgcc acaccaccac gtcacagctc atggcgtctc tgccccggcg
      601 caggatcacg tccccggagg ctaccagtcg ccccttatct cccgcataat acaggacttc
      661 ttcgctgccc agggtgatct tttcctcttt cagatccgcc gataccgccc cccgaaggac
      721 gaagaccgag ccatccgtcc ggcactccgc cgtcttggcc gtcatgatgg attccccctg
      781 gcgcagcgtc acatgtccct gggccaagac cttttgcatg gaaggatcgt agttcaacga
      841 gtcggcgtcc aactgcaaag gaaacccctc acccttgccc cccagggtca gcaccaacac
      901 caaaaggatt cccagaattt ttcgtttctt tcgcattttc tcgaccccct ttgccacggg
      961 agaacgccct tgccccctga atcatcgcac cggatgcgcc aaaacaagcc cccagggccg
     1021 cgacgcctcc atggcgcccg gaccccgggg gctattgtaa cacttcctcc ggtcgatttc
     1081 tggggcgccg tcacgaagaa gcgccctcgc gccccgggag gctccaaagg cctcatgcgc
     1141 cgataaagtt cgcgtggagg gccctcacag cctcctcaat ctggtgggcc gacaccacgc
     1201 acgtgatgga tagcgacgtg gaagcgatca tgtcgatgtt cactcccaat ttgccgagta
     1261 cggagaacat tcgcgaggga acatccgcat ggtgagcgat acctgccccc acgatggaga
     1321 cccgtcccac ttccgtatcg accaaaatcc cttgggactc cacccggcgg gcaaaggcct
     1381 ggcaggtctc cagcgcctcc cccaggatct cttttttcac caagaaggcg atgtcattca
     1441 cgtcccctcg catgacgctc tggatgatca tctccaccgc caatcccctt tgccccagcg
     1501 ccgaaaatag ctccgccgcc acgccgggtt tgtccggaac ccccataacc gccaccttgg
     1561 ccacgttccg atcgtgcacc acagcctgca ccaccaggcc ttcggaaata tcttcccgca
     1621 taatccacgt tccctcctct tcttcgaagg acgaaaggac caccaccggc acgttgaagc
     1681 gcatggccat ctccacgctc cttgcctgga ggatcttggc ccccaacatg gccatctccg
     1741 cacacacgtt gaagggcacc cgggccatct tgcgcgcctc gggaaccacc cgagggtctg
     1801 cggtaaacac ccccgagacg tccgtgtata tataacacac atcggcctcc agggccgccg
     1861 ccagagccac cgccgagagg tcggatcctc cccgccccag agtaat
//
LOCUS       DAOOER010000182         1905 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_60770_length_1905_cov_7.834595, whole genome shotgun sequence.
ACCESSION   DAOOER010000182 DAOOER010000000
VERSION     DAOOER010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60770_length_1905_cov_7.834595"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..534
                     /locus_tag="PL162_10410"
     CDS             160..534
                     /locus_tag="PL162_10410"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006583313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HQF92471.1"
                     /translation="MLYTLVVEGMRFHAFHGSLEVERELGQVLDISINFSYRAEPGEE
                     DLPEKTFPYQKAYEIVQSIVMNTKFRTAEALALAISRQLLKGCPTGEFVQTTIKRGQL
                     YVPGVLDHVGVTVEVCREDLED"
     gene            539..>1905
                     /locus_tag="PL162_10415"
     CDS             539..>1905
                     /locus_tag="PL162_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HQF92472.1"
                     /translation="MPRPMLRLAAGAALGDRGETLDALREACLEGDQCLRQGASADGS
                     GFGWISLPYADPAPLEDAGAWLSSFDAIIQIGIGGSALGNLMLHNALLHPNHNELPRE
                     RRQRPRFYMADNVDPRDLAGIWDLVDPSRTALVAASKSGSTAETMANFLWLLDRMTAA
                     LGEKEAGARLLVLTDPEQGTFRSFVRETGCRSLPIPSSVGGRYSVLSAVGLAAAAALG
                     ISVRDLLAGAQAMDQTIQERQKSVRENPAWLLAGLSYLHYRQGRNMTVLMPYADGLRD
                     FSEWFAQLWGESLGKEGQGSTPVRALGAIDQHSQIQLYTSGPDDKLYTILNVGEHASR
                     AAVPPSSFASLKSLAYLEGKDLGKILQVEARATAASLAQAGRPVIWAEVPKLDAYCLG
                     GLIYLYQFATALTGFLLDVDPFDQPGVEQGKKYIYGLLGRSGFADAAAQVEEHAAAME
                     TLRLE"
BASE COUNT          356 a          660 c          575 g          314 t
ORIGIN      
        1 acatggtggc ccgcaccggc cagatgggcg tccccgtcat cgacatcgac ggcacgttca
       61 tcattggctt cgacaaagga gccctcgacg ccaaactcgc ggaaaagaga catcagggct
      121 aagcagtttt tgccagacac gggagaggag ctgaccgctg tgctatacac cctggtcgtc
      181 gaaggcatgc gctttcatgc gttccacgga tccctggagg tggaacgcga gctgggacag
      241 gttttggaca tttcgataaa tttctcgtat cgtgctgaac ccggagaaga agacctcccc
      301 gaaaagacct tcccctacca gaaggcctac gagatcgtcc aaagcatcgt gatgaacacc
      361 aaattccgca ccgccgaggc cctggccctc gccatctccc ggcagcttct caaggggtgc
      421 cccacgggag aattcgtcca gaccaccatc aaacggggcc agctctatgt ccccggagtg
      481 ctggaccacg tgggcgtcac cgtcgaagtc tgccgggaag atctggagga ctaggaccat
      541 gccccggccc atgcttcgcc tggcggcggg agccgccctg ggagaccggg gagagaccct
      601 ggatgccctg cgggaagcct gcctggaagg agatcagtgc ctgagacaag gagcctccgc
      661 cgacggctcc ggcttcggct ggatctccct cccctatgcg gaccccgctc cgctggagga
      721 cgcgggggcg tggctctctt cctttgacgc catcatccag atcggcatcg ggggctccgc
      781 tctgggaaat ctcatgctcc acaacgccct gctccatccg aaccacaacg agctcccccg
      841 ggaacgacgc caaagacccc ggttctacat ggcggacaac gtggatcccc gggatctcgc
      901 gggaatctgg gatctggtgg atccctcccg caccgccctg gtggcggcga gcaaatccgg
      961 cagcaccgcc gaaaccatgg ccaatttcct gtggcttctg gaccgcatga ccgccgctct
     1021 gggggaaaaa gaggctgggg cgcgcctgtt ggtgctcacc gaccccgaac agggcacctt
     1081 ccggtccttt gtccgggaaa cgggatgccg cagcctcccc atcccctcct ccgtgggggg
     1141 acgctactcc gtcctctccg ccgtgggcct cgccgccgcc gccgccctgg gcatctccgt
     1201 gcgggatctc ctggcggggg cccaggccat ggaccagacc atccaggagc gccagaaaag
     1261 cgtacgggaa aaccccgcgt ggctcctggc cgggctttcg tacctgcact atcgccaggg
     1321 gcgaaacatg accgtgctca tgccctacgc cgacggcctc cgggattttt ccgagtggtt
     1381 cgcccagctc tggggcgaaa gcctcggcaa ggaaggccag gggagcaccc ccgtccgggc
     1441 cctgggagcc atagaccaac actcccagat ccagctctac acctcggggc ccgacgacaa
     1501 gctctacacc atcctcaacg tgggagaaca cgcctcccgc gccgccgttc ccccctcctc
     1561 cttcgccagc ctcaaatcgc tggcctatct ggaggggaaa gacctgggca agatcctcca
     1621 ggtggaagcc cgggccaccg ccgcatctct ggcccaggcg gggcggcccg tgatctgggc
     1681 ggaagttccc aaactcgacg cgtactgtct ggggggactc atctatctgt accagttcgc
     1741 cacggccctc accggatttc tcctcgacgt ggatcccttc gaccaacccg gcgtggagca
     1801 ggggaaaaag tacatctacg gccttctggg gcggtcgggg ttcgccgacg ccgccgccca
     1861 ggtggaagaa cacgccgccg ccatggagac cctgcggctg gagtg
//
LOCUS       DAOOER010000183         1883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_61909_length_1883_cov_7.409190, whole genome shotgun sequence.
ACCESSION   DAOOER010000183 DAOOER010000000
VERSION     DAOOER010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61909_length_1883_cov_7.409190"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..1226)
                     /locus_tag="PL162_10420"
     CDS             complement(141..1226)
                     /locus_tag="PL162_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005662240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HQF92473.1"
                     /translation="MGASNVSMQALLAEVLRSGASDLHLSVGFPPAFRLDGRLHMATQ
                     IEPLTTEDLWRYAESVLSTRQLEDIRRNKDLDFSFGYTPPTGEGARFRGNCYYERSNL
                     TMALRTISNRIRTLKQLGMPPLLTEVAQKSRGLFLATGPTGSGKSTTLAAIIQEINVQ
                     RRCHVVTVEDPIEYLFTSERAIIHQREVGFDTPSFAEALRRVLRQDPDVIMIGEMRDL
                     ETVGAAVTAAETGHLVLSTVHTPDAPQSIDRIVDVFPPHQQNQVRLQLSNILIGLCSQ
                     QLIPLPGGGRLCATEILYATPAVRNCIREGKVSQLKSILQTSLAQGMHTMDQDLARHV
                     REGRIAMEEAMTYSSDPRDLERLVFDM"
     gene            complement(1378..>1883)
                     /locus_tag="PL162_10425"
     CDS             complement(1378..>1883)
                     /locus_tag="PL162_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HQF92474.1"
                     /translation="IRDTVTAEMAIRAALTGHTVLSTLHTNDAPSSVIRLIDMGVAPF
                     LVSSSMMAAIAQRLVRRLCTQCKEPYTLPQALCDSLGFPPGTVAYRSVGCEACRNSGF
                     KGRTAIFEIMVVNDEIRNLIVRGAPLSDIRSLAIQEGMRTLRDQGLRKVRSGVSSLEE
                     VLFVTMG"
BASE COUNT          354 a          554 c          604 g          371 t
ORIGIN      
        1 ttctttggcc caaaagacgt caaaaaaata gacattgcta ctgtctgttg acaccgcttt
       61 ctccgacccg aaaggcgatt catcgaaccc cccgaagaaa gggcgcgatg cccctggcag
      121 aggtgggttt ccgcattttt tcacatatcg aagaccagcc gctccaggtc ccggggatcg
      181 gaggagtagg tcatggcctc ttccatggcg atacgcccct ccctcacgtg acgggccagg
      241 tcctggtcca tggtatgcat cccctgggcc aggctggtct gaaggatgct tttgagttgg
      301 gaaaccttgc cttctctaat gcaattgcgt accgccggag tagcgtagag gatttccgtg
      361 gcacagagcc gccccccccc ggggaggggg ataagttgct ggctgcaaag ccctatgagg
      421 atattgctga gctggaggcg cacctggttc tgttggtgcg ggggaaagac gtccacgatg
      481 cgatcgatgc tctggggggc gtcgggggtg tggacggtgg aaagcaccag gtgtcccgtc
      541 tccgctgcgg tgacggcggc ccccacggtt tccagatccc gcatttcccc gatcatgatg
      601 acgtccggat cctggcgtag cacccggcgc agggcctcgg caaagctggg agtgtcaaag
      661 cccacctccc gctggtggat gatggcccgc tccgaagtga aaagatactc gatggggtcc
      721 tccacggtga ccacgtggca tcggcgttgg acgttgatct cctggatgat ggcggcgagg
      781 gtggtactct tgccgcttcc cgtaggaccc gtggcgagaa agagaccccg ggacttctgg
      841 gccacttccg tgaggagggg gggcatgccg agctgtttca gggtccggat gcggttggag
      901 atggtccgaa gggccatggt gagattgctc cgctcgtagt agcagttccc ccggaagcgg
      961 gccccctctc ccgtgggagg ggtgtacccg aaactgaaat ccagatcctt attgcggcgg
     1021 atgtcctcca actgccgggt agaaaggacg ctttccgcgt agcgccagag gtcttccgtc
     1081 gtgagaggtt cgatctgagt agccatgtgt aaccggccgt ccaggcgaaa cgccggggga
     1141 aaacccacgc tcaggtgcag gtcgctggca ccgcttcgca gcacttctgc cagaagggcc
     1201 tgcatggata cgttcgaggc tcccatgtct tcccgtcctt ccctgccgtt ttttccgccc
     1261 cgtctccccg ggccgaggcc ccgttagctc cggagatctc tcgccccttc gcccatcaag
     1321 aaccaggacc aatgggaatt ttatccatgg gaaggcggcg gcacaccaag gggcacccta
     1381 tcccatggtg acgaagagca cttcctccaa ggaactcacg ccgcttctca ccttgcgcag
     1441 cccctgatcc cggagggtgc gcatcccttc ctggatcgcc agggatcgga tgtccgaaag
     1501 gggagctcct cgaacgatga ggtttcgtat ttcgtcgttc accaccatga tctcgaagat
     1561 ggcggtacgt cccttgaaac cgctgtttcg gcaggcctcg caccccacgg agcgataggc
     1621 cacggtgccc ggagggaaac ccagggaatc gcaaagggcc tggggaaggg tgtagggttc
     1681 cttgcactgg gtgcataggc gtcgcaccag gcgctgggca atggccgcca tcatggaaga
     1741 agacaccaaa aagggcgcca cacccatgtc tatgagacgg atgacggagc tgggagcatc
     1801 gttggtgtgc agggtggaga ggaccgtatg ccccgtgagg gccgcccgga tggccatttc
     1861 ggcggtgacg gtgtcccgga tct
//
LOCUS       DAOOER010000184         1849 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_63681_length_1849_cov_5.363991, whole genome shotgun sequence.
ACCESSION   DAOOER010000184 DAOOER010000000
VERSION     DAOOER010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1849
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63681_length_1849_cov_5.363991"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="PL162_10430"
     CDS             <1..446
                     /locus_tag="PL162_10430"
                     /inference="COORDINATES: protein motif:HMM:NF014310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92475.1"
                     /translation="LLRGGGKVCGILTEAASDPDQIHFAVVGMGINVNLCGGLVPKDI
                     SPTPPALLQREGDPLIHRGWLVVAILEELVSLVSLLESGGSQGLVAEYESHCATLGRA
                     VQVITDQGITLGTARGISSEGALVVESAEGVRQFASADVIHASLG"
     gene            533..1549
                     /gene="selD"
                     /locus_tag="PL162_10435"
     CDS             533..1549
                     /gene="selD"
                     /locus_tag="PL162_10435"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:566..568,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HQF92476.1"
                     /translation="MKLMDRSRTSGUAAKIGPADLEVLLRDLGHPQHNRLLAGWDRGE
                     DAALWDLGNGQVAILTVDLITPVTNDPYLWGQVAAANALSDVFAMGGAPRVALNVVAF
                     PVSCEPLETLRAVLEGGRDKIGEAGALLAGGHSLDDEEPKYGLVVFGEGAKDRLWRVS
                     GARPGDGILLTKPLGTGIMITAVKAGLASAEEQEALEGTMATLNDLPRRLPDSIAPAL
                     RACTDVTGFGFAGHLLDMVGESLDGEVDLAALPLLPGVMGKSALGLVPAGCYRNREAF
                     GGRVREMRKYDAHESDILFDPQTSGGLLLAVDPAAEDAVAALIPGTRRVGRFVEGSGG
                     LMVR"
BASE COUNT          331 a          505 c          657 g          356 t
ORIGIN      
        1 atctgctccg aggagggggt aaggtgtgcg gcatcctcac ggaggccgcc agcgaccccg
       61 accaaatcca ttttgccgtg gtaggcatgg ggataaacgt caacctctgc ggggggttgg
      121 tcccgaaaga catctccccc actcctccgg ccctcctgca gcgagagggc gaccccctga
      181 tacaccgggg gtggttggtc gtagccatcc tcgaagagct tgtgtccttg gtttctttgt
      241 tagaatctgg gggaagccaa ggcctggtgg cggaatacga gagccactgt gccaccctcg
      301 ggagagccgt ccaggtgatc acagaccaag gaatcactct gggcacggcg cgggggattt
      361 cctccgaagg agctttagtg gtggagtccg ccgaaggggt gcggcaattt gcctcggcgg
      421 acgtgatcca cgcctccttg gggtgacgtc gcggccccct aaggggactt gaggagccca
      481 ggatattctt tattcgatgc gggtaatgga tgataagggg gtgatcggca tggtgaagct
      541 catggaccgc tctcgtacca gtgggtgagc ggcaaagata ggtccggcgg acctggaggt
      601 tcttttacgc gatttggggc acccccaaca caaccgcctg ctggcgggat gggaccgagg
      661 agaagatgcg gctctctggg atttgggaaa cggccaagtg gccatactta cggtggatct
      721 catcactccg gtgaccaacg acccctatct ttgggggcag gtggctgcgg ccaacgcctt
      781 gagcgacgtt tttgccatgg gaggagctcc tcgggtggcc ttgaacgtgg tggctttccc
      841 cgtgagttgt gagcccctgg agacgctgcg tgccgtcctt gaaggaggaa gggacaagat
      901 cggggaggcc ggggcgttgt tggctggggg gcacagtttg gacgacgagg agcccaaata
      961 tgggctcgtg gtctttggag aaggggccaa agatcgcctt tggcgtgtct ctggagcccg
     1021 cccgggggat gggatccttt tgaccaaacc acttgggacg ggcatcatga tcaccgcagt
     1081 gaaggcgggc ttggcgagcg cggaagaaca ggaggcctta gagggcacca tggccaccct
     1141 caacgacctt ccccgccgcc tcccggattc catagctccc gcccttcggg cctgcaccga
     1201 cgtgacgggg ttcgggttcg cgggccatct cttggatatg gtgggggaaa gtctggatgg
     1261 ggaagtcgac ctagccgccc tacccctcct gccaggggtc atgggaaaat ctgccctggg
     1321 cctggtcccc gccggatgtt atcgcaatcg agaggccttc gggggcaggg ttcgggagat
     1381 gagaaaatac gatgcccatg agagcgacat cctttttgac ccccagacct ctggcgggct
     1441 gctcttggcc gtggatcccg ccgcagagga cgcggtggcg gccctgatcc ctgggacccg
     1501 tcgcgtgggg cgtttcgtgg aggggtccgg gggcctcatg gtgaggtgac gggcggggtc
     1561 tgccccctta tgccgcaagg cggggcgggc cccgaaggtt cctatcggac caaagggcct
     1621 gcccatctca agggaacgat gccccccgga gccttctttt ctgggaaaag gccccggggg
     1681 gtggactttt cggcgtaacc ccacggtttt aggggaagga tgtcctttgc aaaggcgtgg
     1741 cccaagggac ggcgatcggg gaggatgtcc cgggggtcag caatcgagtt ctttatacga
     1801 aagaagggtg gcggcatgga cgcgggcctc gggagaaagg gggtctccg
//
LOCUS       DAOOER010000185         1804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_66209_length_1804_cov_5.444254, whole genome shotgun sequence.
ACCESSION   DAOOER010000185 DAOOER010000000
VERSION     DAOOER010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66209_length_1804_cov_5.444254"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..1359
                     /gene="pepT"
                     /locus_tag="PL162_10440"
     CDS             136..1359
                     /gene="pepT"
                     /locus_tag="PL162_10440"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009164794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HQF92477.1"
                     /translation="MSVALEKFLRYVTFDTQSAEGTGQVPSTPGQWELARFVAQELKS
                     MGLEDVRVSDHAYVTATLPANTDKAIPAIGLIAHLDTALEVTGRDVRPRVVSYEGGDI
                     VLNEALGVVFSPRDFPDLLDYVGQDLVVTDGTTLLGSDDKAGMAAIMGALDFLTTHPE
                     VVHGPVKVAFTPDEEIGHGADLLDLEGFGADFAYTVDAGPLGQINFETFNAARADIRI
                     HGRAVHPGTSKNKMLNATLLGHELLNLMPPAETPATTEGYEGFYHVTGFSGSVEEGSL
                     SFIIRDHDRHRFEERKAFVRRAVEWMRGKYGKERFDLEISDTYYNMREPLEKAMHIVE
                     TAKEAMTSLGIAPKVVPIRGGTDGSRLSYRGLLCPNIFYGGHNAHGKFEFLPVESLDK
                     SVRVVLKILELYAAK"
BASE COUNT          316 a          567 c          605 g          316 t
ORIGIN      
        1 gccccggagt ggacgccacc caccactacg agcggaccca ccggaaagcc ctggaagaga
       61 ccgcccggct gctggtggcc tatctgacgg catgatcggg tccttcgggg ggcccgagag
      121 aggagaatga gaaccatgag cgtcgctctg gaaaaatttt tgcggtatgt gacctttgat
      181 acccagtccg ccgagggcac ggggcaggtc cccagcaccc ccgggcagtg ggagctggcg
      241 cgtttcgtcg cccaggagct gaaaagtatg ggccttgagg acgtgcgggt gagcgaccac
      301 gcctacgtca ccgccaccct tccggccaat acggacaagg ccatccccgc catcggcctc
      361 atcgcccacc tggacaccgc cctggaggtc acgggccggg acgtgcgccc ccgggtggtg
      421 tcctacgagg gaggcgatat cgtgctgaac gaggccctgg gggtggtctt ttctccccgg
      481 gacttccccg acctcctaga ctacgtgggg caggacctgg tggtcaccga cggcaccacc
      541 ctactgggct ccgacgacaa ggcgggcatg gcggccatca tgggggccct ggactttctc
      601 acgactcacc ccgaggtggt ccacggcccg gtgaaggtgg cctttacgcc ggacgaggag
      661 atcggccatg gggcagatct cctggatctc gaaggttttg gggcggattt cgcctacacg
      721 gtggacgcgg gacctctggg acagatcaac ttcgagacct tcaacgccgc ccgggccgac
      781 atccgtatcc acggccgggc ggtgcacccc ggcacgtcca agaacaagat gcttaacgcc
      841 acgctcttgg gccacgagct cctgaacctc atgcctccgg cggagacccc cgccaccacc
      901 gaagggtacg agggcttcta ccacgtcacg ggattctccg gatcggtgga agaggggtcc
      961 ctgtcgttca tcatccggga tcacgaccgc catcgcttcg aggagcgcaa ggccttcgtg
     1021 cgccgggccg tggagtggat gcggggcaag tacggcaaag agcgcttcga tctggaaatc
     1081 tcagacacgt actacaacat gcgggagccc ctggagaagg ccatgcacat cgtggaaacc
     1141 gccaaggagg ccatgacctc cctgggaatt gcccctaagg tggtgcccat ccgggggggc
     1201 acggatgggt cccgcctatc ctatcgaggg ctcctgtgcc ccaacatctt ctacggaggg
     1261 cataacgccc acggcaagtt cgaatttctc cccgtggagt ccctggataa gtccgtgcgg
     1321 gtcgtgctca agatcttgga gctctacgcc gcgaaatagc cctccgctcc cgccccttcg
     1381 ggggtgggag tttctttttt ctccccccag gggcccgggg agggaaatgg cggaggaggc
     1441 gccgccaggg aggcggagcg aaggcttcgc ggggcgatga aagagctttc ggggcccgtt
     1501 tagggtttag ggggctcctc cctccttccc acgccgtccc tcccgtgtta ccatgacatg
     1561 tggaggagtt tttcttcgcg gggcgaccgg acccggggga gcccgatccc ccgtcggagg
     1621 agggcgtcgg agtaggaaag gaggtggggg cgtgcaggaa cattttctgg ccttttctgt
     1681 ggcggagcgg cgctttgccc tgccccttgc cgaagtggat cgcatcgtgg ctgccgtgga
     1741 gatccaaggc cttccccagg cgcccgcctt tgtccgagga gtggtgaacg tgggaggagt
     1801 gctg
//
LOCUS       DAOOER010000186         1779 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_67654_length_1779_cov_4.823086, whole genome shotgun sequence.
ACCESSION   DAOOER010000186 DAOOER010000000
VERSION     DAOOER010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67654_length_1779_cov_4.823086"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(166..1206)
                     /locus_tag="PL162_10445"
     CDS             complement(166..1206)
                     /locus_tag="PL162_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92478.1"
                     /translation="MDDGRGPGRGAWLARRLFPALGACALVGGLLGVLRAETLAFPGI
                     QGMAAGALLGCTLGWNARGDAPACWPFRARLALGLGAIPTYLAFHLGVFSFALARGAD
                     SPFFWLGEIAGGFQKEAFFSLGVTGPVYRAFQGVMTGGGWIFFNLLDGIFFGVFLLIL
                     LGVGLGRRFELPAEDSGAEMVSPLSRSFLMAVVCSYLVLGGVGLWKLWHAVEVGPPLS
                     FSPASWRALERYEGPWRLVEDSPAIFRASGDRLFFLRVAGFEDLAGQSASGRFSFSVT
                     RRGSTYQGLLYTGRREGKTWHPSGGAYPLRLRLAPDASFLEGAVTLFTVNGRRDGRFR
                     GHPLSGDPGLGE"
     gene            complement(1251..1691)
                     /locus_tag="PL162_10450"
     CDS             complement(1251..1691)
                     /locus_tag="PL162_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92479.1"
                     /translation="MEVRLLSKSGQQPQWIRVVLLVLGMLVLGHALQRYLMTTKDSLP
                     SFLVGGACFFLAGLRKRLYIAPEGVVREIRCWGKTRETLLPWGDVEHVTLVFFKEDMM
                     AFFELRGSVRGWRLYFRRDDEGPLRRLIKEYIPGVEVATQEKRF"
BASE COUNT          366 a          674 c          497 g          242 t
ORIGIN      
        1 ggtcgtgcac catgaggtct cctacggcgg agaggcgcac cggggccccc tcccctggga
       61 ctatccccac ccctatccat gccaccagta ccagcccctt tacccacttc catccccgca
      121 ccgtcgccgc cccctttctt tttcgccgcc ttcggtccct tgtccctact ctcccaagcc
      181 cgggtccccc gacagggggt gcccccggaa acgtccgtcc cggcgcccgt tcacggtaaa
      241 aagggtcacc gccccctcca aaaaagaggc gtccggggcc agccgcaagc gaaggggata
      301 cgctcccccc gaggggtgcc acgttttgcc ctcacgcctc cccgtgtaca ggagcccttg
      361 ataggtgctc ccgcgccggg tgaccgaaaa ggaaaaacgt ccggaggcgc tttggcccgc
      421 caggtcctcg aaccccgcca cccgcagaaa aaagagccgg tcgccggagg cgcgaaaaat
      481 cgccggagag tcctccacca ggcgccaggg gccctcgtag cgctccaggg cccgccacga
      541 cgcagggcta aaggaaaggg gcggccccac ctccaccgcg tgccacagct tccagagtcc
      601 cacgcccccc aggacaaggt aggagcacac caccgccatc agaaaggagc gggaaagcgg
      661 ggaaaccatt tccgctccgg agtcctcggc cgggagctcg aagcgccgtc ccagccccac
      721 ccccaggaga atcagcaaga agacgccaaa aaagatccca tccagcaggt tgaaaaaaat
      781 ccaccccccg ccggtcatca ccccctgaaa ggcccgatag acggggcccg tcacgccaag
      841 agaaaaaaac gcttccttct ggaagccccc cgcgatctcc cctagccaaa agaagggaga
      901 gtctgctccc cgggccaggg cgaaggaaaa aacgccgagg tggaaggcaa gataggtggg
      961 gatggcgcca agccccaggg ccagccgcgc ccgaaagggc cagcatgccg gagcgtcccc
     1021 ccgggcgttc cagcccaggg tgcatcccaa aagagccccc gccgccatgc cctgaatgcc
     1081 gggaaaagcc agagtttccg cccggagcac ccccaaaagc ccccccacca gggcgcacgc
     1141 ccccagcgcg gggaaaaggc gcctcgctaa ccaggctccc cggcccggcc ctctcccgtc
     1201 gtccatggtc tttcctcctc aaatccttcc ccccgggcgc tttggcgagg tcagaacctt
     1261 ttttcctgag tggccacctc taccccgggg atgtattctt taatcagacg gcgcaggggg
     1321 ccctcgtcat cgcgacggaa gtagagccgc cacccccgga cgctcccccg gagctcaaaa
     1381 aaggccatca tgtcctcttt gaagaagacc aacgtcacgt gctccacgtc gccccaggga
     1441 aggagggtct cccgggtctt tccccagcag cggatctccc gcaccacccc ttccggggcg
     1501 atgtacaggc gcttcctcag gcccgccagg aaaaagcagg ctccccccac caggaaggag
     1561 gggagagagt ccttggtggt catcaggtag cgctggaggg cgtgccccag gaccaacatg
     1621 ccaaggacta gcagcaccac gcggatccac tggggctgtt gtcccgattt cgacagcaat
     1681 cgcacttcca caagccatcc cccccttctt cgcgcccccg gcggaggcgt tcttacataa
     1741 atgtggggag gcgcggccct tcgccacccc tccccacgg
//
LOCUS       DAOOER010000187         1775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_67902_length_1775_cov_8.987209, whole genome shotgun sequence.
ACCESSION   DAOOER010000187 DAOOER010000000
VERSION     DAOOER010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67902_length_1775_cov_8.987209"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..1499
                     /locus_tag="PL162_10455"
     CDS             123..1499
                     /locus_tag="PL162_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006301074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HQF92480.1"
                     /translation="MNVTTMMVESLARLVRPGGAWHADAFAQYASGHGMFFEDGKIEQ
                     TSSFGAGGIGVRALVEDHAVFSHAPGVSSRAAQQGLRDLEELLGLPFPSPEVFQDSLL
                     VDPIELEASPSWDFAREVDQDLRRASSAVRQVSLSFRTSLSSVKIFRSDGKMAEERRP
                     YTTFSLSVVVGKDATLQTGSETRALRVPSKIFLEGFDPRAMGHSALERALIMLDAPPC
                     PAGPMPVILAGEAGGTMIHEACGHGLEADIVQKDFSVYRDALDSMVSTPCVTLVDDPT
                     LPDLYGSYAVDDEGTASQRTILVDQGMLRRYLTDLQSARRGGLPLSGNGRRESYRHLP
                     IPRMSNTYVMPGTTPLEDMLAQAERGLFVRRLGGGEVDPTSGDFVFAVTEGYLVEKGR
                     VGSPVRGATLTGNGPEVLRRILAVGSSLHMDPGSCGKNGQGVPVTDGQPTLLLESLVV
                     GGSDSSHA"
     gene            1543..>1775
                     /locus_tag="PL162_10460"
     CDS             1543..>1775
                     /locus_tag="PL162_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92481.1"
                     /translation="MGRPQDTVAKTSAPRRTRDSDRRSGKKNPSPTARRRRSPSTPDL
                     GRRLLVLLGVLGGIFLLAGFWTPWAGPRGTAWAS"
BASE COUNT          316 a          548 c          563 g          348 t
ORIGIN      
        1 gcgctctttc gggcgaacct tcggcgccct tcgttctcga agcggtcttg ggcgaaaaaa
       61 agaccccata cgccccggcc ttacctccgt tgaggccctc tggggcgggg aaaggatctg
      121 acatgaacgt aacgacgatg atggtagaat ccctggcacg cctggttaga ccgggaggtg
      181 cgtggcatgc ggacgccttt gcccaatatg ccagcgggca tgggatgttc ttcgaggacg
      241 gcaagataga acagacctcg tccttcggcg ccggggggat cggggtacgg gccctggtgg
      301 aagaccacgc ggtcttttcc cacgccccgg gagtatcctc tagagcggcc cagcaaggac
      361 ttcgagacct agaggaactc cttggtctgc cttttccttc cccagaggtt ttccaagact
      421 ctcttctggt cgatcccatc gaattggagg cctctccatc ttgggatttt gcccgggagg
      481 tggatcaaga ccttcgccgg gcctcctcgg cggtacgcca ggtttccctg tcctttcgga
      541 cttctctttc ttcggtgaag atttttcgaa gcgacgggaa gatggcagag gagcggcgcc
      601 cctacaccac cttctctctg agcgtcgtgg tgggcaagga tgccaccctt caaacgggga
      661 gcgaaacccg ggccctcagg gtcccttcga agatctttct ggagggcttt gaccctcgtg
      721 ccatggggca cagcgccctg gagcgggccc tcatcatgct ggacgctccc ccctgcccgg
      781 cgggccccat gccggtcatc ctggcggggg aggcgggggg aaccatgatc cacgaagcct
      841 gcggccacgg ccttgaggcg gacatcgtac aaaaggactt ttccgtatat cgagatgctc
      901 tagatagcat ggtgtctacc ccctgcgtaa ccttggtgga tgaccccacg ctaccggacc
      961 tctacggaag ctacgccgtg gatgacgagg gaacggcttc ccagcggacg atcctggtgg
     1021 accaggggat gctgcgccgt tacctcaccg acctacaatc cgcccgccgg ggagggcttc
     1081 ccctttcggg aaacggcagg agggaatcct atcgccatct ccccattccc cggatgtcca
     1141 acacctacgt gatgccggga accactccct tggaggacat gttggctcag gcggagcgag
     1201 gcctctttgt ccgccgccta ggagggggag aggtggatcc cacttcggga gacttcgtct
     1261 tcgccgtgac ggaggggtat ctggtggaga aggggcgcgt gggatccccg gtacgcggag
     1321 ccacgctcac gggaaatggc cccgaggtac tacgccgtat tttggctgtg gggtcctctc
     1381 ttcacatgga tcccggctct tgcggaaaga acggacaggg ggttccggtt accgacggcc
     1441 agcccacgct ccttttagag agcctagtgg tgggaggcag tgactcctcc catgcctagg
     1501 cttcgcaccg acgtccccag acaggacggg gagggaagct ccgtggggcg tccgcaagat
     1561 actgtcgcaa aaacctcggc acctcgccga acccgggaca gcgaccgccg atcggggaaa
     1621 aaaaatcctt cgcccaccgc tcgacggcgc cgctctccct cgactccaga cctggggcga
     1681 cgactcctgg tccttttagg agtcttggga ggaatatttc ttttggcggg gttctggacc
     1741 ccctgggcgg gtccccgggg aacggcgtgg gcgtc
//
LOCUS       DAOOER010000188         1747 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_69598_length_1747_cov_4.432624, whole genome shotgun sequence.
ACCESSION   DAOOER010000188 DAOOER010000000
VERSION     DAOOER010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69598_length_1747_cov_4.432624"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..913
                     /locus_tag="PL162_10465"
     CDS             <1..913
                     /locus_tag="PL162_10465"
                     /inference="COORDINATES: protein motif:HMM:NF018010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HQF92482.1"
                     /translation="MAADEGELTRVRPTPQGPETQRLRVNFRQALAGDPAADILLEIN
                     DFLIVRSVPEWRLYTTVSVAGEVRYPGTYTINPGERLSDVLERAGGLTSGAFPRGTIF
                     TRASVRRQQTITKNEMVDRMERELLQQAGVASSQTAGSGEAAEAATSATQLRAFLQRL
                     RQAPVTGRLVVRVPEDFRLLKGSPYDVEMEQGDSITIPRRMMSVQISGAVYTPLSQIY
                     RPGLSYRQYIAQAGGFTPTADPRRIYVLRADGSAMRVTTGKKALAIEEGDTIVVPERI
                     EVLSKLRQTRDLVDMIYKIAASVVVFK"
     gene            924..>1747
                     /locus_tag="PL162_10470"
     CDS             924..>1747
                     /locus_tag="PL162_10470"
                     /inference="COORDINATES: protein motif:HMM:NF014732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HQF92483.1"
                     /translation="MAHDRETLQERQAPAPENQVDASSQEDDVDLLDLLLVFVRHWKL
                     IFVVTFLFAAGGLTYALMATPIYRAEARLVPPSGGGSSGAMAMLAQLGGAADFAAGAL
                     GVKTPADLLVGLVKSRTVVDRIIAQFSLMERYNQEKMTSCRTTVLGILSANADKQSGI
                     ITLAYEDKSPDQAAAIANAFVLELKDLLKGLAVTQASQQRLFLEEQLKKSQLDLLQAE
                     NSLQAYQQSTGILNVDAQMAALLQRVAQLKALIAAKEVELRSARTFATARNPEIKKL"
BASE COUNT          333 a          584 c          551 g          279 t
ORIGIN      
        1 catggcggcg gacgaggggg agctcacccg ggtacgcccc accccccagg gccccgagac
       61 ccagcgcctg agggtgaact ttcgccaagc cctggcgggg gaccccgccg ccgacatcct
      121 cctggagata aacgacttcc tcatcgttcg ttccgtcccc gagtggcggc tctacaccac
      181 ggtgagcgtg gcgggggagg tgcgctaccc cggcacctat accatcaacc ctggcgagcg
      241 cctctcggat gtgctggaac gggccggggg cctcacctcg ggggcctttc cccggggcac
      301 gatcttcacc cgggccagcg tccggcgcca gcaaacaatc acaaaaaacg aaatggtaga
      361 ccgcatggag cgggagctcc tgcagcaggc gggagtggcc agctctcaaa cggcgggctc
      421 gggggaagcg gcggaggcgg cgacctccgc cacccagctg cgcgcctttc tccagcgcct
      481 tcgccaggcc cccgtgacgg ggcgcctggt ggtgcgcgtg cccgaagact ttcgcctcct
      541 caaagggagc ccctacgacg tggagatgga gcaaggtgac agcataacca ttccccgccg
      601 catgatgtcg gtgcagatct ccggggcggt gtacaccccc ctatcccaga tctaccgccc
      661 ggggctttcc taccgccagt acatcgccca ggccggaggg ttcaccccca cggcggatcc
      721 caggcggatc tacgtcctcc gggccgatgg ctcggcgatg cgggtcacga cgggcaaaaa
      781 ggccctggcc atcgaagaag gggacaccat cgtggtcccc gaacgcatcg aagtcctcag
      841 caaactgcgc cagacccgag acctggtgga catgatctac aagatcgccg ccagcgtcgt
      901 ggtcttcaag tagggggacg accatggccc acgatcgtga aacactccag gagcgccagg
      961 cccccgctcc cgaaaatcag gtggacgctt cttcccagga agacgatgtc gaccttttgg
     1021 accttttgct ggtcttcgtc cgccactgga agctcatctt tgttgtgacg tttctcttcg
     1081 ccgccggggg gctcacctat gcgctgatgg ccactcccat ctaccgggcc gaagctcgcc
     1141 tggtgccccc gtcggggggg gggagctcgg gggccatggc catgctcgcc cagctggggg
     1201 gagcggcgga ctttgccgcc ggagccctgg gggtaaagac cccggcggat ctgttggtgg
     1261 ggttggtgaa aagtcgtacc gtggtggacc ggatcatcgc gcaattttcc ctcatggaac
     1321 ggtataacca agaaaagatg acatcctgcc ggactacggt gctggggatc ttgagcgcga
     1381 atgcggataa gcaaagcggc atcatcaccc tggcctacga ggacaagagc cccgatcagg
     1441 cggcggccat cgccaatgcc tttgtcctgg agctcaaaga cctcctgaag ggcctggccg
     1501 tcacccaggc gtcccagcag cgcctctttc tggaagagca gctgaaaaaa tcccaactcg
     1561 acctgctcca ggcggaaaac agcctccagg cgtaccagca gagcacgggg atcctgaacg
     1621 tggacgccca gatggcggcc ctgctgcagc gtgtggccca gctcaaggcc ctcatcgccg
     1681 ccaaggaggt ggagcttcgg tccgcccgga cctttgccac cgcccgaaat ccggagatca
     1741 aaaaact
//
LOCUS       DAOOER010000189         1728 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_70837_length_1728_cov_5.177525, whole genome shotgun sequence.
ACCESSION   DAOOER010000189 DAOOER010000000
VERSION     DAOOER010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70837_length_1728_cov_5.177525"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1728
                     /gene="thrS"
                     /locus_tag="PL162_10475"
     CDS             <1..>1728
                     /gene="thrS"
                     /locus_tag="PL162_10475"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HQF92484.1"
                     /translation="WQCDKGAVAARVNGRMVDLFSPVSEDASVEPVEGASEEGLEILR
                     HSAAHLMAQAVQRLYPGAKFGVGPAIKDGFYYDMELPRRLLEEDLPRIEDEMRRLAQE
                     NIPVTRREFSRDEALAFFRDRGQSYKEELIAEIPEGEVLSCYEQGDFADLCRGPHVPS
                     TGAVRHFKLLSVAGAYWRGSEQNPMLTRVYGTAWGSAEGLKHHLEMLEEIEKRDHRRL
                     GRDLDLFSVHEEAGPGLIYWHPKGGRLRVELENWWRQEHFAHGYDILFTPHLGRSWLW
                     ETSGHLGFYKENMYSPMEIDKDDYYVKPMNCPFHILVYKTRTRSYRDLPLRWAELGTV
                     YRYERSGVLHGCMRVRGFTQDDAHIICTPEQIEDEILEVLRFSMYVWRTLGFSEVKAY
                     LATRPEKSVGDDASWQTAAASLRKAIEAEGLSYEVDEGGGAFYGPKIDLKVRDAVGRE
                     WQVTTIQFDFNLPERFDMTFTDADGKEKRPFMVHRALLGSLERFFGILIEHYAGAFPY
                     WLAPVQARIIPVASDHGAYAAEVEKTLRKWGVRVEVDARDEKLGKRIRDAQMQKIPYM
                     LVIGDRERD"
BASE COUNT          319 a          538 c          577 g          294 t
ORIGIN      
        1 ggtggcagtg cgataagggg gcggtggcgg cccgggtgaa cggccggatg gtggacctct
       61 tttcccccgt gtccgaggac gcctccgtgg agcccgtgga gggcgccagc gaagagggtc
      121 tggagatcct gcgccactcg gcggcccacc tcatggccca ggccgtgcag cgcctttacc
      181 ccggggcaaa attcggcgtg ggccccgcca tcaaggacgg gttttactac gacatggagc
      241 tcccccgtcg cctcctcgaa gaggatttgc cccgtatcga ggatgaaatg cgccgtttgg
      301 cccaggaaaa catccccgtg acccggcggg agttctcccg ggacgaggcc ctggcgttct
      361 tcagggatcg gggacagagc tacaaggaag aactcatcgc ggagatcccc gagggggagg
      421 tgctctcctg ctacgaacag ggagacttcg ccgacctgtg ccgggggccc catgtgccct
      481 ccacgggggc cgtgcggcac ttcaagctcc tctccgtggc gggggcctac tggcggggga
      541 gcgagcagaa ccccatgttg acccgggttt acggcaccgc ctggggaagc gcggaaggat
      601 tgaagcacca cctggaaatg ctcgaagaga tcgagaagcg ggaccaccgg cgcctgggac
      661 gcgatctgga cctcttctcc gttcacgagg aggcgggccc cggcctcatc tactggcacc
      721 ccaagggggg gcgcctgcgg gtggagctgg agaactggtg gcgccaggag cactttgccc
      781 acggctacga catcctcttt acccctcatc tgggacgctc ctggctctgg gagacctcgg
      841 ggcatctggg tttctacaaa gaaaacatgt actcccccat ggagatcgac aaggacgatt
      901 actatgtcaa gcccatgaac tgccccttcc acatcctggt gtacaagacc cgcacccgga
      961 gctaccgcga tctgccgctc cgttgggcgg agctgggcac ggtgtatcgc tacgagcgat
     1021 cgggggtgct ccatggttgc atgagggtcc ggggctttac ccaggatgac gcccacatca
     1081 tctgcacgcc ggagcagatc gaagatgaga tcttggaggt gttgcgcttc tccatgtacg
     1141 tctggcgcac cctaggtttt tcggaggtaa aggcctattt ggccacccgg ccggaaaagt
     1201 ccgtgggaga cgacgcctcc tggcagaccg ccgccgcctc cctccggaag gccatcgaag
     1261 ccgaagggct gtcctacgag gtggacgaag ggggcggagc cttctacggc cccaagatcg
     1321 acctgaaggt acgagacgct gtggggcggg agtggcaggt gaccaccatc cagttcgact
     1381 ttaacctccc cgagcgcttc gacatgacct tcaccgacgc ggacggcaag gaaaagcgcc
     1441 ccttcatggt gcaccgggcc cttctgggct ccctggagcg cttctttggc attctcatcg
     1501 aacactacgc gggggccttt ccctactggc ttgcccccgt gcaggcccgg atcatccctg
     1561 tggcgtcgga ccacggggcc tacgccgccg aggtggaaaa gaccctgcgg aaatggggag
     1621 tgcgggtgga agtggacgcc cgggacgaga agctcggcaa gcgcatccgg gacgcccaga
     1681 tgcagaagat cccctacatg ttggtcatcg gggaccggga gcgcgacg
//
LOCUS       DAOOER010000190         1709 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_72084_length_1709_cov_3.778718, whole genome shotgun sequence.
ACCESSION   DAOOER010000190 DAOOER010000000
VERSION     DAOOER010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72084_length_1709_cov_3.778718"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /gene="dctP"
                     /locus_tag="PL162_10480"
     CDS             complement(<1..598)
                     /gene="dctP"
                     /locus_tag="PL162_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HQF92485.1"
                     /translation="MFKKRFLALAVCVAVLGIFLASSAFAAPEMTLKFAGQSPADHTA
                     TKFMEDIAKEVAEKSGGRIEIKVYPANQLGDYTLVYEELIRGTIDMAGISIPSQFDPR
                     MELVYINGFVRGYEDAKRIFTPTGWLFKKMDELNQALGVKLVGFYVEGFIGTGTTKPA
                     KDPLDPTVEKGLLIRVPNMDVYMLAAQAMGYKTITIPYA"
     gene            complement(1110..>1709)
                     /locus_tag="PL162_10485"
     CDS             complement(1110..>1709)
                     /locus_tag="PL162_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HQF92486.1"
                     /translation="PVMRLLTTEAERRIRPVPRPEHSVSRDVRIIFPIAVTILASVVA
                     PASASLIGFLMLGNLIRECGVLERLSRGAQNELANIVTLLLGISISATMTGEQFLRAD
                     TLVVIAMGLVAFIFDTAGGVITAKVMNLFLKEKINPFIGAAGISAFPMASRVVQRMGQ
                     EYDRGNHLLMHAAGANVAGQIGSVLAGGLLLSFIGRMAG"
BASE COUNT          343 a          509 c          538 g          319 t
ORIGIN      
        1 cggcgtaggg gatggtgatg gtcttgtagc ccatggcctg ggcggcgagc atgtacacgt
       61 ccatgttggg cacccggatg agcaggccct tttccacggt gggatcaagg gggtccttgg
      121 cgggcttggt ggtgccggtg ccgatgaaac cctccacgta gaagcccacg agcttcacgc
      181 ccagggcctg gttgagttcg tccatcttct tgaagagcca tcccgtgggg gtgaaaatgc
      241 gcttggcatc ctcgtagccg cgcacgaagc cgttgatata gacgagttcc atgcgggggt
      301 caaactggct gggaatggaa atccccgcca tgtcgatggt gccgcggatg agttcttcat
      361 aaaccagggt gtagtccccc agctggttcg cgggatagac cttgatctcg atgcggccgc
      421 cggacttctc cgccacttcc ttggcgatgt cttccatgaa ctttgtcgcc gtgtgatccg
      481 ccggggactg gccagcgaac ttgagagtca tctccggtgc ggcaaaggcg gaagaagcca
      541 agaagatccc caaaaccgcc acacagaccg ccagcgcgag aaaacgcttt ttgaacatgg
      601 tcaccgataa catctccttc ccgaaggtgc gaaaaattcc ccactgcccc gttcccaccg
      661 ccacgacttt cggcggtgcc ccgctccgtg gtccgtcgcg ccgctgcctg agccccaccc
      721 ccttcgatga gcatccaccg ccgcagggga aaagatgccg ccgtccccct agggtctccc
      781 ttttgtacca ttgatgaggc gttccatcaa ggcccgattc gccgctttcc ccttaccgcc
      841 ggacagggga catgcttcga agaacttcat ggcgctcaga agaggggagc ttatcgccgg
      901 gccgcgtaga aacatcagac atatccccca cgccattata agcaagggtt tcctctttga
      961 ctaggggatg ccccgcagga cagtggatct ggagaggaga aatattgtgt ggagcatacg
     1021 aaaaacggcg ttctcctccc cttgtaaggg ggagaacgcc gaagatgttc aggaaagggg
     1081 ttcttgctcc caagagagcg tttcggagct taccccgcca tgcgaccgat gaaagaaagc
     1141 aagagtcctc cggcgaggac cgagccgatc tgccctgcca cgttggcccc cgcagcgtgc
     1201 atgaggaggt ggtttccccg atcgtactcc tgccccatcc tctgcaccac ccgggatgcc
     1261 atggggaagg cggaaatgcc tgcagcgccg atgaaggggt tgatcttttc cttcagaaag
     1321 aggttcatga ccttggcggt gatcacgccc ccggcggtgt caaagatgaa ggccacaagc
     1381 cccatggcga tgacgaccaa ggtgtcggcg cgcaggaatt gctcccccgt catggtggcg
     1441 ctgatggaga tgcccagaag cagggtgacg atgttggcca gttcgttctg ggctccccgg
     1501 gagaggcgct ccagcacccc gcactcccgg atgaggttcc ccagcatgag aaagccgatg
     1561 agggaggcgg aggcgggggc caccacgctg gccagaatgg tgacggcgat ggggaagatg
     1621 atgcggacat cgcgggacac ggagtgttcc ggccggggca cggggcgaat ccgccgctcg
     1681 gcctccgtgg tgaggaggcg catcaccgg
//
LOCUS       DAOOER010000191         1674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_74455_length_1674_cov_4.852378, whole genome shotgun sequence.
ACCESSION   DAOOER010000191 DAOOER010000000
VERSION     DAOOER010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74455_length_1674_cov_4.852378"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="PL162_10490"
     CDS             complement(<1..1063)
                     /locus_tag="PL162_10490"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HQF92487.1"
                     /translation="MDDGRDLVDLIIGGDPREAPAVESEFLADFLTEAREHLERIELD
                     VLELENAPESEEIVHSIFRSFHTLKGLAGFVGQTLVAKVAHRTETVLDDCRKKILRPG
                     KGVVEGILRSADLIQRLCSHMALGQNRAFLEEVAAHVDRLDRRAFSSDSPEAPEKGTL
                     EGAPPRAPLTPSADLPGAPSPGEEPLRPPAPPAPEPGTAPPSRGASPASPRPASSGEG
                     SPGGGASVRVATAKVDSMVDMLGELLITQSQIEQEAVRRFGQNDPLVTHLGRLSRITK
                     ELQSLSMSLSMVSLKSTFQKLRRLGRDTVASLGKEVDLQFVGEDTEIDRGVAEKLLDP
                     LVHLLKNAISHGVEDEKTRL"
     gene            complement(1101..1547)
                     /locus_tag="PL162_10495"
     CDS             complement(1101..1547)
                     /locus_tag="PL162_10495"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HQF92488.1"
                     /translation="MDLRESLLRSTPETLGLFAVQAEPAGEMEQPGLTSAHQVNVLVG
                     LSQGGRGNVVLGMSRKGALKIVGGMMGGMAVTELDDLGKSALAELGNMLVGSGVRAVS
                     TPEGLDISPPTVVLGDRVFLFISRVPSRVLNFRTEDGPFAVRFSLE"
BASE COUNT          276 a          591 c          543 g          264 t
ORIGIN      
        1 cgaggcgggt cttttcgtcc tccacgccgt gggagatggc gtttttcagc aggtgcacca
       61 gggggtccag gagcttttcc gccacccccc ggtcgatctc cgtgtcctcg ccgacgaact
      121 gaagatccac ctccttgccc aacgaggcca cggtgtcccg ccccaacctc cgaagtttct
      181 ggaaggtgga tttaagggaa accatgctga gggacatgga aaggctctgc aattccttgg
      241 tgatgcggga aaggcgcccc aggtgggtca ccaggggatc gttctggcca aagcgccgta
      301 ccgcctcctg ttcgatttgg gattgggtga tgaggagctc tcccagcatg tccaccatgc
      361 tgtccacctt ggcggtggcc acccggacgc tcgccccccc tcccggagat ccctctccgg
      421 aggaggcggg gcgaggggac gccggggagg ccccccggga agggggagcc gtcccaggct
      481 ccggcgccgg aggggccggg ggacgaaggg gctcctcccc cggggaggga gcccccggga
      541 ggtcggcgga gggagtgagg ggggctcggg ggggcgcccc ctcgagagtt cccttctctg
      601 gggcctcggg ggagtccgac gagaaggccc ggcgatcgag gcggtccacg tgggccgcca
      661 cctcctccag gaaggcccgg ttctggccga gggccatatg ggagcagagg cgctggatga
      721 gatccgccga gcgcaggatc ccctccacca cgcccttgcc gggacgaagg atcttctttc
      781 ggcagtcgtc cagcacggtc tcggtgcggt gggccacctt cgccacgagg gtctgcccca
      841 cgaagcccgc cagccccttc aaggtatgaa aggagcggaa gatgctgtgt acgatctcct
      901 cgctctccgg ggcgttttcc aattccagga cgtccagttc gatgcgctcc aggtgctccc
      961 gggcttcggt gagaaaatcc gccaaaaact cggactccac cgccggggcc tcccgggggt
     1021 cgccgccgat gatcaggtcc accagatctc gtccgtcgtc catgttttcc ctccgtcctt
     1081 cggggcgccc ggccgagggg tcactccagg ctgaaccgca ccgcaaaggg gccgtcttcc
     1141 gtacggaagt tcagcacccg ggaggggacc cgactgatga aaagaaacac ccggtccccc
     1201 agcaccaccg tgggagggga aatgtcgagc ccctccgggg tggacaccgc ccggaccccc
     1261 gatcccacca gcatgttgcc cagctccgcc agggcgcttt tccccaagtc gtccagctcc
     1321 gtcaccgcca tgccgcccat catgcccccc acgatcttca gggctccctt gcgggacatg
     1381 cccaggacca cgtttccccg ccccccctgg gagagcccca ccaggacgtt cacctggtgg
     1441 gccgaggtga gccccggctg ttccatctcc cctgcgggct cggcctggac ggcgaagagc
     1501 cccagggttt cgggggtcga tcgcagcaaa gactcccgaa gatccaaccc tcacaccccc
     1561 tcaaaaccca cgtcgaagcg ggccacgccc cgggcccccc gataccatgt cacctgggat
     1621 cgcaccaggg gggtgaggat ctccagcccc ttgcccgcga agatcgccgg cgga
//
LOCUS       DAOOER010000192         1663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_75257_length_1663_cov_3.590174, whole genome shotgun sequence.
ACCESSION   DAOOER010000192 DAOOER010000000
VERSION     DAOOER010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75257_length_1663_cov_3.590174"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..641)
                     /locus_tag="PL162_10500"
     CDS             complement(204..641)
                     /locus_tag="PL162_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92489.1"
                     /translation="MARQSLTGSLWVLGAAALLIWAGAAGANPSGASGDRAPTAEQRA
                     LAERVREVGAVRVMVTLNVPFVPEGELKSAWQVFRQRERIAQAQERVEGRLRTSGCAP
                     LGPVKRFDSVPQMALRVGGPGLEALWEDPDVKEVREDALAATH"
     gene            complement(738..>1663)
                     /locus_tag="PL162_10505"
     CDS             complement(738..>1663)
                     /locus_tag="PL162_10505"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HQF92490.1"
                     /translation="AMNGELYDPATPDGFAVICHELGHQFCKLPDLYDTSNTNQGLGA
                     FSIMAAGSWGCRSGDTPGSTPTNMDAWCRLYLGWETPSSLVAGVVTLGVPGNGTHPAL
                     RLYGAGHRPTEYFLAEVRGLVGWDRGLEGLSRNGGFLGLEGGILVQHVDEEVGAGRLE
                     GGNDFNTYVAGGHQGNMAVEADGPHMAKTDDTATRGSRYTLWYQGNPNYVGDGTLTGS
                     STPSTAFYDGSASGVSLTEISAGGVTMTCRVTGGEPLPTPTPTLAPTATPRPTLRPTP
                     GGSSGGGGCFAAETPWTLLLLLPLLGAVVGK"
BASE COUNT          261 a          627 c          499 g          276 t
ORIGIN      
        1 ccgccttggc ccgcagggcc gccgggttat ccaggcgaca ggtggcctcc gcccccatcg
       61 ctccccagag gaaggccccc acaagaagtc ccgccatcac gtgccgcacc gtcattttaa
      121 ccatgattca acctcctcat cgtattattc acaccaaact cctccgccca ggacccgcgc
      181 ccttcaatgc ccttcaatgc ccttcaatgg gtcgccgcca gggcatcctc ccggacctcc
      241 ttcacgtcgg ggtcctccca gagggcctcc agaccggggc cgccgacccg cagggccatc
      301 tggggcacgc tgtcgaagcg cttcaccggt cccagggggg cgcatcccga cgtcctcagg
      361 cgaccttcca cccgctcttg ggcctgggcg atgcgctccc tctggcgaaa gacctgccag
      421 gcacttttca gttccccttc ggggacaaag gggacattca gggtgaccat aacccgcacc
      481 gcccccacct cccggacccg ctccgccagg gcccgttgct ccgccgtcgg ggcccggtcc
      541 cccgaagctc ccgagggatt cgcccccgcc gccccggccc agatcaaaag ggccgccgct
      601 cccaagaccc aaagggatcc tgtcaaactc tgccgcgcca caacatcgcc ccctttcctc
      661 ggaccattat aaggcttttt ctgcggcgca caatgggctt ttgtcccctt ttcgaccccg
      721 ggcccccttg ggtccgccta tttcccgacc accgccccca gaaggggcag gaggagcagc
      781 agagtccagg gggtctcggc ggcaaagcag ccgccgccac cggagctccc ccccggcgtg
      841 gggcgaaggg tgggcctcgg cgtggccgtg ggagccaggg tgggagtggg ggtaggcaag
      901 ggttcgcccc ccgtcacccg gcaggtcatg gtgactcccc ccgccgagat ttccgtcaaa
      961 gaaacccccg aggcggagcc gtcgtagaag gccgtggagg gcgtgctgct ccccgttaac
     1021 gtcccgtctc ccacgtagtt cggatttccc tggtaccaga gggtataccg cgacccccgg
     1081 gtggcggtgt cgtccgtttt cgccatgtga ggaccatcgg cctccaccgc catgttgccc
     1141 tggtgtcccc ccgccacgta cgtgttgaag tcgttgccgc cttccagcct ccccgcgccc
     1201 acttcttcat ccacgtgctg cacgaggatg cctccctcaa gccccaggaa gcccccattc
     1261 cgggaaaggc cctccaggcc tcggtcccac cctacgaggc cccgcacctc cgccaggaag
     1321 tactccgtgg gccgatgccc cgcgccgtag agccgaaggg cggggtgggt gccgttgccg
     1381 ggaaccccca gggtcaccac ccccgcgaca agggacgagg gcgtttccca ccccaggtag
     1441 aggcggcacc aggcgtccat gttggtgggg gtgcttccgg gggtgtcccc cgaacggcat
     1501 ccccagctcc ccgccgccat gatggaaaag gccccgagcc cttggttggt gttggaagtg
     1561 tcgtatagat cggggagttt gcagaactga tgccccagct cgtggcagat tacggcgaag
     1621 ccgtcggggg tggcggggtc gtagagctcc ccgttcatgg ccc
//
LOCUS       DAOOER010000193         1615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_78767_length_1615_cov_6.210256, whole genome shotgun sequence.
ACCESSION   DAOOER010000193 DAOOER010000000
VERSION     DAOOER010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78767_length_1615_cov_6.210256"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            188..1384
                     /locus_tag="PL162_10510"
     CDS             188..1384
                     /locus_tag="PL162_10510"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HQF92491.1"
                     /translation="MLREEPLAPRWSGEEGDGKFLTSLATPRVLLVDDSQVLRTFLRR
                     FLARAVPGGDFVEASDGNEALKVLESGDVDLVITDLVMPELDGFGVLEALRDRWPDIP
                     VIVVSGLDDPESLEQALSRGAFDYLTKPFKAREVEIVLPLKVRNALGKHARSRQLRQL
                     HQALQQELQKASAFVRDVLLPPVPEGWKGATWYRPAMEVGGDLYYWTERDGALWFIVA
                     DAMGHGVEAALNLALMRCLFLEGTARGGGPEDVLSFVNARLCRSSQGDDLGMYITAAV
                     GKIRGLEGVVASAGHPSPLLLRRDGTVSPLSSGGFVLGAFPEASYPGSSPFTMGEGDA
                     LLLFTDGALPRRWNAETWRGWVEFAWREHQGRPLEELLASLWQGGAALEPPEERDDVT
                     MALLAP"
     gene            1431..>1615
                     /locus_tag="PL162_10515"
     CDS             1431..>1615
                     /locus_tag="PL162_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92492.1"
                     /translation="MAFYEKGAADDREPCPGCACSRGAFAGAEGSDREDGGELRRSLG
                     SLLGAVGLGVGGYVLRQG"
BASE COUNT          239 a          477 c          609 g          290 t
ORIGIN      
        1 tcaagacatc aagctcttcg gccccgaggc ccaagagctc ttggcccacg tgcggaggtg
       61 cgatcctcag gggggagcat gagggccttc cccccggggg cctttggttt gggatccccg
      121 gggtctttgg gaaaaggggc gaaaaggtag agggacttcg gctcccgacg gaaaggacgt
      181 ggtgcgcgtg cttcgagaag aaccgctagc gccgagatgg agcggggagg aaggcgacgg
      241 gaagttcctg acatccctgg cgacgccccg ggttctcctg gtggacgatt cccaggtgct
      301 acggaccttt cttcgaaggt tcctcgcccg agccgtgccc gggggggact tcgtggaggc
      361 ctccgatggg aacgaggccc tgaaggttct ggaatccggc gacgtggatt tggttatcac
      421 cgatctggtg atgcccgagc tcgacggctt cggcgtgctg gaggccctgc gggatcgctg
      481 gcccgacatc ccggtgatcg tggtctccgg cctggacgat cccgaaagcc tggagcaggc
      541 cctttcccgg ggggccttcg actaccttac taaaccattt aaggcgcgag aggtggagat
      601 cgtccttccc ctgaaggtgc gtaacgccct ggggaagcac gcccggagcc gccagctgcg
      661 ccagctgcac caggctcttc aacaggagct ccagaaggcc tcggccttcg tccgggatgt
      721 cctccttccc cccgtccccg aggggtggaa gggggccacg tggtaccgtc ccgccatgga
      781 ggtggggggc gatctctact actggacgga gcgggacggc gccctctggt tcatcgtggc
      841 ggacgccatg ggacacgggg tggaggcggc gctaaacctg gccctgatgc ggtgcctctt
      901 tttggaaggg acggcccggg gcggggggcc cgaggacgtg ctttccttcg tgaacgcgcg
      961 tctctgtcgg tcgtcccaag gggacgactt agggatgtac atcaccgctg cggtgggaaa
     1021 gatccggggc ctcgaggggg ttgtcgccag cgcggggcac ccttcgcccc tgttgttgcg
     1081 gcgggatggc accgtgagtc ccctttcctc cggggggttt gtgctgggag ctttccccga
     1141 ggcttcctac cccgggtcct cccctttcac catgggggag ggggacgccc ttttgctctt
     1201 cacggacgga gccctccccc gtcgttggaa cgccgaaacc tggcggggtt gggtggagtt
     1261 cgcctggagg gagcaccaag gccgtcccct ggaggaactc ttggcgtcgc tgtggcaagg
     1321 gggggcggcc ctcgagcccc cggaggagcg ggatgacgtg acgatggctt tgctcgcccc
     1381 ctagggggat ttaagggctt gagtcccacg gagggaaagg agaccctgac gtggcttttt
     1441 acgaaaaagg cgccgctgac gaccgggagc cctgcccggg gtgcgcctgc tcccgggggg
     1501 ccttcgcggg ggcagaggga agcgatcggg aggacggggg ggagctccgg aggtccctgg
     1561 ggtctctcct gggggcggtg gggctcgggg tcggggggta cgtcctgcga caggg
//
LOCUS       DAOOER010000194         1610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_79142_length_1610_cov_5.785852, whole genome shotgun sequence.
ACCESSION   DAOOER010000194 DAOOER010000000
VERSION     DAOOER010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79142_length_1610_cov_5.785852"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1610)
                     /gene="thrS"
                     /locus_tag="PL162_10520"
     CDS             complement(<1..>1610)
                     /gene="thrS"
                     /locus_tag="PL162_10520"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HQF92493.1"
                     /translation="WQCDKGAVAARVNGRMVDLFFSVSGDASVEPVGGASEEGLEILR
                     HSAAHLMAQAVQHLYPGAKFGVGPAIKDGFYYDMELPRRLLEEDLPRIEDEMRRLAQE
                     NIPVTRREFSRDEALAFFRERGQSYKEELIAEIPEGEVLSCYEQGDFADLCRGPHVPS
                     TGAVRHFKLLSVAGAYWRGSEQNPMLTRVYGTAWGSAEGLKHHLEMLEEIEKRDHRRL
                     GRDLDLFSVHEEAGPGLIYWHPKGGRLRVELENWWRQEHFAHGYDILFTPHLGRSWLW
                     ETSGHLGFYKENMYSPMEIDKDDYYVKPMNCPFHILVYKTRTRSYRDLPLRWAELGTV
                     YRYERSGVLHGCMRVRGFTQDDAHIICTPEQIEGEILEVLRFSMYVWRTLGFSEVKAY
                     LATRPEKSVGDDASWQTAAASLRKAIEAEGLAYEVDEGGGAFYGPKIDLKVRDAVGRE
                     WQVTTIQFDFNLPERFDMTFTDADGKEKRPFMVHRALLGSLERFFGILIEHYAGAFPY
                     WLAPVQARIIPVASDHGAYAAEVEKTLR"
BASE COUNT          288 a          545 c          495 g          282 t
ORIGIN      
        1 ccgcagggtc ttttccacct cggcggcgta ggccccgtgg tccgacgcca cgggaatgat
       61 ccgggcctgc acgggggcaa gccagtaggg aaaggccccc gcatagtgtt cgatgagaat
      121 gccgaaaaag cgctccaggg agcccagcag ggcccgatgc accatgaagg gacgtttttc
      181 tttgccgtcc gcgtcggtga aggtcatgtc aaagcgctcg gggaggttaa agtcgaactg
      241 gatggtggtc acctgccact cccgtcccac cgcatcccgc accttcaggt cgatcttggg
      301 gccatagaag gccccgcccc cctcgtccac ctcataggcc agcccctcgg cctcaatggc
      361 cttccggagc gaggcggcgg cggtctgcca ggaggcgtcg tcccccacgg acttttccgg
      421 ccgggtggcc agataggctt ttacctcgga aaagcccagg gtacgccaga catacatgga
      481 gaagcgcaac acctccagga tttccccctc aatctgctcc ggcgtgcaga tgatgtgggc
      541 gtcgtcctgg gtgaaccccc ggaccctcat gcaaccgtgg agcaccccgg aacgctcata
      601 gcggtacacg gtgcccagct ccgcccagcg cagcggcaga tcgcggtagc tccgggtgcg
      661 ggtcttgtac accagaatgt ggaaggggca gttcatgggc ttgacgtagt agtcgtcctt
      721 gtcaatctcc atgggggagt acatgttttc tttgtagaac cccagatgcc ccgaggtctc
      781 ccacagccag gaacgcccca gatggggagt gaagaggatg tcgtagccgt gggcaaagtg
      841 ctcctggcgc caccagttct ccagctccac ccgcaggcgc ccccccttgg ggtgccagta
      901 gatgaggccg gggcccgcct cctcgtgaac ggagaagagg tccagatcgc gtcccaggcg
      961 ccggtggtcc cgcttctcga tctcttcgag catttccagg tggtgcttca atccttccgc
     1021 gcttccccag gcggtgccgt aaacccgggt caacatgggg ttctgctcgc tcccccgcca
     1081 gtaggccccc gccacggaga ggagcttgaa gtgccgcacc gcccccgtgg aagggacatg
     1141 gggcccccgg cacaggtcgg cgaaatctcc ctgctcgtag caggagagca cctccccctc
     1201 ggggatctcc gcgataagct cttccttgta gctctgcccc cgctcccgga agaacgccag
     1261 ggcctcgtcc cgggaaaact cccgccgggt cacggggatg ttttcctggg ccaaacggcg
     1321 catttcgtcc tcaatacggg gcaaatcctc ttcgaggagg cggcggggga gctccatgtc
     1381 gtaataaaat ccatccttga tggcgggccc cacgccgaat tttgccccgg ggtaaaggtg
     1441 ttgcacggcc tgggccatga ggtgggccgc cgagtggcgg aggatctcca aaccctcctc
     1501 gctggcgccc cccacgggct ccacggaggc gtctccggac acggagaaaa agagatccac
     1561 catccggccg ttcacccggg ccgccaccgc ccccttatcg cactgccacc
//
LOCUS       DAOOER010000195         1595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_80365_length_1595_cov_4.375325, whole genome shotgun sequence.
ACCESSION   DAOOER010000195 DAOOER010000000
VERSION     DAOOER010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1595
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80365_length_1595_cov_4.375325"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..537)
                     /locus_tag="PL162_10525"
     CDS             complement(<1..537)
                     /locus_tag="PL162_10525"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HQF92494.1"
                     /translation="MKQKIVPLAFFLTWVLAFALEGGHPAAVAAQAFFTDDPEYLSMQ
                     RLGDRRYAPEMASIIAHFFLKRNPRLDFPTAHAFARRILKASKMQGVDPLLVAAIVVK
                     ESNANPKDRGGGCIGLMQIKWKAHRTSIPKAFPHVKKEKDLYSPYYNIDIGTYLFANY
                     LKMSHFNTDAALNRYKGAD"
     gene            869..1366
                     /locus_tag="PL162_10530"
     CDS             869..1366
                     /locus_tag="PL162_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92495.1"
                     /translation="MVVGALFLGEERALGEEISPSSFQPLAPLLREVVGWEGDLLVLE
                     LDADGRGGSLGRSYARGGETAEVRVLVGPEVKNYLGGMLPGVALMESAGLRLERFAEG
                     SFDVVLRSEKETKGGAVVVILSSEDRGALVVEYEGLSPDAALALAREFDWRGMADRLP
                     EGVRE"
     gene            1384..>1595
                     /locus_tag="PL162_10535"
     CDS             1384..>1595
                     /locus_tag="PL162_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92496.1"
                     /translation="MRDFRTGGMVKIGRGILGWAVSLMILVLFPAGAYSQGAPVGGGA
                     EVAPSQVLLRYLVDRLQPESADLVLDR"
BASE COUNT          272 a          384 c          596 g          343 t
ORIGIN      
        1 gtcggcgccc ttgtagcgat tcagggcggc atcggtgttg aagtggctca ttttcaggta
       61 gttggcaaag aggtacgtcc cgatgtcgat gttgtaatag ggactgtaga gatctttctc
      121 cttcttgacg tgggggaagg ccttggggat gctggtgcgg tgggccttcc atttgatctg
      181 catgaggccg atacaccctc ctcctcggtc cttggggttg gcgttggatt ccttgaccac
      241 gatggccgcc acaagtagtg ggtccactcc ctgcatttta gaggccttga ggatccttcg
      301 ggcgaaggcg tgagcggtgg gaaaatccag ccgagggttg cgtttgagga agaaatgggc
      361 gatgatggag gccatttcgg gggcgtacct tcgatctccg aggcgttgca tggaaaggta
      421 ttccgggtca tcggtgaaaa aggcctgggc cgcaaccgcc gcaggatgac cgccctccag
      481 ggcgaatgcc agaacccacg tcaagaaaaa agccaggggg acgatttttt gcttcaaaag
      541 atttcactcc tctcctttcg ggtgagtgcc ggttgatggg catgggcgat atgttacgca
      601 aaagggggct ctttggcaac tagggaattt ataaatgctt gccctgtaag cctttatgcc
      661 gatttttccc ctgacgagag gggccctttc tggtagaatg gcccgaatga tcatgggatc
      721 ccgagacgat gggggtgggg ttgtggaaga gagggccgag ggaacgaccg agagggcatg
      781 ctccccgatc ccttcgctcg acccttcccc ggggtctttg tggagtgaag gcggtaggag
      841 cgcgtggcgc cggggcgcgc gggccctgat ggtcgtgggg gccctttttc taggagagga
      901 aagggccctc ggggaggaga tctcgccttc gtccttccag cccttggcgc cccttctccg
      961 ggaagtggtg ggatgggaag gcgacctttt ggtgctggag ctcgacgccg acggccgagg
     1021 gggttcgctg ggaaggagct acgcccgagg gggcgagacg gcggaggtcc gggtgttggt
     1081 ggggcctgag gttaaaaact acctgggagg catgctcccg ggggtggcgc tcatggaaag
     1141 tgccgggctt cggctggagc gttttgccga agggagcttt gatgtggtgc ttcggagcga
     1201 aaaagagacg aaggggggcg cggtggtggt gatcctctca tcggaagacc gaggagccct
     1261 ggtggtggag tatgaagggc tttcccccga cgcggcgctg gcgctggccc gagaattcga
     1321 ctggcggggc atggccgacc gtctccccga gggggtgcga gagtgaaggg gatgggacgt
     1381 cgcatgaggg actttcggac cggcggcatg gtgaagatcg ggcgagggat cctgggatgg
     1441 gcggtgagcc tgatgatcct cgtccttttt ccggcggggg cctattctca gggggctccg
     1501 gtcggcgggg gggcggaggt tgccccttcc caggtgctct tgcgttatct ggtggatcgc
     1561 cttcagcccg aatcggcgga tctggtgctg gatcg
//
LOCUS       DAOOER010000196         1575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_82082_length_1575_cov_4.775658, whole genome shotgun sequence.
ACCESSION   DAOOER010000196 DAOOER010000000
VERSION     DAOOER010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82082_length_1575_cov_4.775658"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            309..>1575
                     /locus_tag="PL162_10540"
     CDS             309..>1575
                     /locus_tag="PL162_10540"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014806276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HQF92497.1"
                     /translation="MLFSENALTILKKRYLKNDETPEALLRRVARAVASAEAEGDRPL
                     WEDRFFALMERLDFLPNSPTLMNAGTPQGQLAACFVLPVGDSMEEIFSTLKNTALIHK
                     SGGGTGFNFSSLRPAGDVVSSTNGVASGPVSFMGMFDLATDVVKQGGTRRGANMAILN
                     GDHPDVLDFIRAKTEEGKLANFNISVGLSDDFWKTLERDGEWTLKNPRTGAPVRTLPA
                     REIFDRIVHCAWATGDPGVIFSDRLNRDNPTPHLGPIVSTNPCGEQPLLPYEACNLGS
                     VNLAHMVTPERTVDEARLEEAVRLGVRFLDNVIDVNSYPLPEIREMSQGNRKIGLGVM
                     GWAEMLFRMGIPYDSEGALALGGRVMAQIQSVGHDESRRLAQERGAYPNCRQEVRRNA
                     TVTTIAPTGTISLLAGCSSGIEPVFALVHT"
BASE COUNT          288 a          522 c          474 g          291 t
ORIGIN      
        1 caaaagctct ggggatcgtt gagtccttcg aggtttgcct ttctgatttc gtctgccgtg
       61 acttttttca tttgatttcc cctccctctt tgatcttgag tatattatac ctcgcaactt
      121 acgctagcgc aataggtcta aagacccatc ttttgcgctg ccgttcgatt ccccccggct
      181 ccacccctac ggatggagta tgatgggtca tgagcgggac ggagggctcg gccccgcagg
      241 accgtgccgg gccccttccc ttccgttgcc ccggggcgac cgggggaatg aataaggagg
      301 tcctttttgt gcttttttcc gaaaacgccc tgaccattct aaaaaaacgt tacctcaaga
      361 acgacgagac ccccgaagcc ctgctccggc gcgtggcccg ggccgtcgcc agcgccgagg
      421 ccgaagggga tcgccccctt tgggaggatc gtttcttcgc cctcatggaa cgtctggatt
      481 tccttcccaa ctcccccacc ctcatgaacg cgggaacccc ccaggggcag ctcgcggcgt
      541 gcttcgtcct ccccgtggga gacagcatgg aagagatctt ctccaccctg aaaaacaccg
      601 ccctcatcca caaatccggc ggaggcacgg ggttcaactt ctcctccctg cgccccgcag
      661 gagacgtggt gagcagcacc aatggcgtgg cctcggggcc cgtgtccttc atgggcatgt
      721 tcgacctggc cacggatgtg gtgaagcagg ggggcacccg ccggggggcc aacatggcca
      781 tcctgaacgg ggatcacccc gacgtgctgg acttcatccg ggccaagacc gaagagggca
      841 agctcgccaa cttcaacatc tccgtggggc tttcggacga tttctggaaa actctggagc
      901 gagatgggga gtggacgctg aaaaaccccc gcacgggggc gccggtgcgc accctccccg
      961 cccgggaaat cttcgaccgc atcgtacact gcgcctgggc cacgggggat cccggggtga
     1021 tcttttccga ccgcctgaac cgggataacc ccacccccca cctgggaccc atcgtttcca
     1081 cgaacccctg cggcgaacag cccctgctcc cctacgaggc ctgcaacttg ggctccgtga
     1141 acctggcaca catggtcact ccggaacgca ccgtggacga ggcccggctg gaagaggcgg
     1201 tgcgcctcgg ggtgcgattc ctggacaacg tcatcgacgt gaatagctac cccttgccgg
     1261 aaatccggga gatgagccag gggaatcgca agatcggcct gggggtcatg gggtgggcgg
     1321 agatgctctt ccggatgggg atcccctatg actccgaagg agccctggcc ctgggaggac
     1381 gggtcatggc ccagatccag agcgtgggac acgacgaaag ccgtcgcctc gcccaggagc
     1441 ggggggccta cccgaactgc cggcaagagg tgcgccgcaa cgccacggtg accaccatcg
     1501 cccccacggg gaccatttcc ctgctggcgg ggtgctcctc ggggatcgaa ccggtctttg
     1561 ccctggttca caccc
//
LOCUS       DAOOER010000197         1550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_84161_length_1550_cov_3.620736, whole genome shotgun sequence.
ACCESSION   DAOOER010000197 DAOOER010000000
VERSION     DAOOER010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84161_length_1550_cov_3.620736"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1550
                     /locus_tag="PL162_10545"
     CDS             <1..>1550
                     /locus_tag="PL162_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQF92498.1"
                     /translation="IALFLWQSGALALPPPSDAGLEREVERLNRALALREEGEALLQD
                     GDEAGALERFRQSLAIFPDESLEKRVWALEERIAPRKGGPLTPPGPTRGEVALVKTFS
                     RDGGVLAVPGGPQIALDPGTLSGEVTFSLRPAQTAPLTLVGGPPQGLSLGYVWDVDLG
                     GAALGAPARISFDLPREARSGDVLLVRPVFSRSGINWTPLPYRRQGDTLLFEVPHFSL
                     LGIVVITLLAPLIPVAFVYFHGGEQWPDSTLFNAEAPFVADLIPDPTGFHIAWTHLLG
                     DGKTGVRDPAGLAAAETAAKTRFTTAEAAIFQDRDRSLASPKLSEVERAKILMDADRR
                     MDGARSDLEKALAKAKADYLVPESVKLIREGLVFGRSYLLSRGFSSPDMLDVFVPKNC
                     GKDDGTLNNRYWGRNYMMVGASLDRDDIFSTTLHEQFHHFQGQYLTAGKANIYLAEAS
                     AALMEREAADHYRQRAGVAIFSDNRFLNGLKEGLNGKPIKTYAGDAGPFQRLGYGLSW
                     FLEYLRDQ"
BASE COUNT          277 a          513 c          479 g          281 t
ORIGIN      
        1 tgatagcttt gttcctgtgg caaagcggcg ccctggccct tcctcccccc tccgacgccg
       61 gtttagagcg agaagtggag cgcctgaacc gggccctggc ccttcgagag gaaggggaag
      121 ccctgctaca agacggggac gaagccgggg ccctggagcg tttccgtcag tccctggcga
      181 ttttccccga cgaaagcctg gaaaagcgcg tctgggccct ggaggaaagg atcgcccccc
      241 ggaagggtgg gcccctcacc ccccccgggc ccactcgggg ggaagtggcc ttggtaaaaa
      301 ctttctcccg ggatggagga gtcttggcgg tgcccggggg accacagatc gccctggatc
      361 ccgggacgct ttcgggagag gtgacctttt ccctccgtcc cgcccagacg gcccccctaa
      421 ccctggtggg gggaccgccc caggggctct ccctagggta cgtctgggac gtggacctgg
      481 gaggagctgc tctgggggcc ccggcgcgga tttcctttga ccttccccgg gaagcccgaa
      541 gcggcgacgt gctgttggtc cggccggtct tctcccggag tgggataaac tggactccgt
      601 tgccctaccg acgccagggg gacaccctgc tcttcgaagt tccccacttc agcctgttgg
      661 gcatcgtggt catcaccctc ctcgcccccc tcatccccgt ggccttcgtg tacttccacg
      721 gcggagagca gtggcctgat tccaccctgt tcaacgccga agcgcccttt gtggcggatc
      781 tgatccccga tcccacgggg ttccacatcg cctggaccca cctcctggga gacgggaaaa
      841 ccggggtgcg cgatcccgca gggctcgcag cggcggaaac ggcggcgaaa actcgtttta
      901 ccaccgccga agccgccatc ttccaggacc gggaccgatc cttggcatcg cccaaacttt
      961 ccgaggtcga gcgagccaag atcctgatgg atgcggatcg tcggatggat ggggcccgaa
     1021 gtgatctgga aaaggccttg gccaaggcca aagcggatta tctcgtcccc gagtcggtga
     1081 agctcatccg ggaagggctc gtcttcggcc gctcctatct gctttcccgg ggattttctt
     1141 ccccggacat gctggatgtc ttcgtgccca aaaactgcgg caaggacgac ggtaccctca
     1201 acaatcggta ctggggacgg aactacatga tggtgggggc gagcctggat cgggacgaca
     1261 tcttctccac caccctgcac gaacagttcc accacttcca gggacagtac ctcacggcgg
     1321 gaaaagccaa tatctacctc gccgaagcct cggcggccct catggaacgg gaagcggcgg
     1381 atcactaccg tcaacgcgcc ggggtcgcca tcttcagcga caaccgcttt ctcaacggcc
     1441 tcaaggaggg cttgaacggc aaacccatca agacctacgc cggagacgcg ggacctttcc
     1501 agcgcctggg atacggcctt tcctggtttc tggagtacct ccgggatcag
//
LOCUS       DAOOER010000198         1532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_85755_length_1532_cov_7.054841, whole genome shotgun sequence.
ACCESSION   DAOOER010000198 DAOOER010000000
VERSION     DAOOER010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85755_length_1532_cov_7.054841"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="PL162_10550"
     CDS             complement(<1..361)
                     /locus_tag="PL162_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQF92499.1"
                     /translation="MKKDLLGWVLAGMLLAGSAGAEAPIAIGHSVSLTGGSSMWGLAE
                     QNALDMLVEELNAAGGVLGRKLEIIHYDNRGDAVESVNVARRLVDDGVVAVIGPAQSG
                     NSIASAPVFENAGIPMVV"
     gene            complement(389..1168)
                     /locus_tag="PL162_10555"
     CDS             complement(389..1168)
                     /locus_tag="PL162_10555"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HQF92500.1"
                     /translation="MPHPPLTEPEYALLALLGEETLPLGAGALRLALEARGFHLSEAT
                     AGRILQRLDRLGYTERCGFQGRRLSPEGRRLYEAHRILQHRMQSARGVMSALNDEDRR
                     VIVETLEVRRALEGESARLAAERATEEDLALLRSLVEDQIASVKAGQSMATSDGVFHR
                     ALARASGNRLLESSIDLLRQDPLWAPLLEGVRRKQGSPLGADHRAVVEAVASRDPQEA
                     RSAMERHLDNVLRDVRLFLESHPEGFGELFDEGIFPGHDAP"
BASE COUNT          246 a          494 c          517 g          275 t
ORIGIN      
        1 tgaccaccat ggggatcccc gcattctcga agaccggggc cgaggcgatg gagttgccgc
       61 tctgggcggg gccgatcacc gccaccacgc cgtcatccac cagtcgccgg gccacgttga
      121 cggattccac cgcgtctcct cggttgtcgt agtggatgat ctccagcttt cttcccagca
      181 cgcccccggc ggcgttgagc tcttccacca acatgtccag ggcgttctgc tccgccagcc
      241 cccacatgga agaacccccc gtgagggaca cgctgtggcc gatggcgatg ggagcctctg
      301 cccccgccga tcccgccagg agcatccccg ccaggaccca tcccaaaaga tctttcttca
      361 tgtcatgaac ctcctctttt tttcgcccct agggggcgtc gtgcccggga aaaatcccct
      421 cgtcgaaaag ctcaccgaaa ccttcggggt ggctttccag gaagagccgc acgtcccgga
      481 ggacgttatc cagatgcctt tccatggcgc tgcgggcctc ctggggatcc cgggacgcca
      541 cggcctccac caccgcccgg tgatccgccc ccagggggct gccctgcttt cgccggacgc
      601 cctccagcag gggggcccac agaggatctt gacgcaagag gtcgatggag ctttccagaa
      661 gcctattccc cgaggcccgg gccagagcgc ggtgaaagac cccgtccgaa gtggccatgc
      721 tctggcccgc cttcaccgag gcgatctgat cttccaccag ggagcgcagc agggccagat
      781 cttcttccgt ggcccgttcc gccgccagcc gggcggattc tccctccagg gcccgccgca
      841 cctccagggt ttccacgatg acccgccgat cttcgtcgtt cagggcggac atgacccccc
      901 gggcggattg catccggtgc tggaggatgc gatgggcctc gtaaaggcgc cgtccctcgg
      961 gggaaaggcg gcgtccctga aagccgcagc gctcggtgta gccgaggcgg tccaggcgct
     1021 gcaagatgcg ccccgctgtg gcttcgctca ggtgaaaccc ccgagcttcc agggcgaggc
     1081 gaagggctcc cgcccccagg ggcagagttt cttcgcccag cagggcgagg agggcgtatt
     1141 cgggctctgt caggggagga tggggcacgg tggatctcct ttcgggctca agctgcccat
     1201 cattggtgag tggcgtggag gattgtgacc gatccgccgg ggtctgtcaa gggggccgga
     1261 gggtccttgg cgtcgatggg aaaaggtggg ggacgatttt ctccttgtct ctcccggccg
     1321 ttccttctat actgggcgaa gtggcaaggc ggagggtgaa gggatgagcc tggcacctcg
     1381 aaggggggaa gggccgtgcg ggtgacgcaa aatctttgga tgtccggcca agggtggcgg
     1441 gatcgctggg aagatggagc gggtcttcct tcggagcgaa gcttcatctt ggccgtgggg
     1501 gatggggagg cctgggagaa tcgggcttgg cg
//
LOCUS       DAOOER010000199         1516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_87261_length_1516_cov_5.857632, whole genome shotgun sequence.
ACCESSION   DAOOER010000199 DAOOER010000000
VERSION     DAOOER010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87261_length_1516_cov_5.857632"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..512
                     /locus_tag="PL162_10560"
     CDS             114..512
                     /locus_tag="PL162_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQF92501.1"
                     /translation="MAEPWRVLVVEDDGAVGNTLVAFLEDEGFDVRLARSGEDALTIL
                     KSGWIPRVGVVDLRLPGMDGRAVVECARVLAPSVRFLVHTGSPGGVVEDLAFSMGVDA
                     KDVFIKPVRDLGLLVERLRRSGEGEDGHGA"
     gene            502..>1516
                     /locus_tag="PL162_10565"
     CDS             502..>1516
                     /locus_tag="PL162_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQF92502.1"
                     /translation="MGRDPGGPKHILVVEDDGAVRRHLTAYLEDCGYRVTAAAEGEGA
                     LEMARTQHPDLVITDLHMPGISGFQVLSALAEEFPTLPVLVVSGTDLLTEVVEALRRG
                     AWDYLIKPLEEMGVLEHAVERALERAWLLEENRRSREELEEAVARRTQELQALLTERT
                     VLLREVHHRVKNNFQVISSLLQIRARSSRHPEVRNFSSDFAQRIRSMALVHDALYQTS
                     DFARWNFGEYLKRLVGDLAWGDGSRSPEVRVEAAPLEVGLDLAVPCALIAHELVSNAL
                     RHAFPEGGGQVIVSLSAETPGMGHLEVRDDGVGLSQETLDQAEGGMGLQIARILVQQL
                     SGTL"
BASE COUNT          251 a          448 c          564 g          253 t
ORIGIN      
        1 cgtgtcgtat ttcatcgtgg tgagccgcca taacgggtgg atgaaggtgg acgatgcccc
       61 cggaggggga gcggtattcc aggtcttttt gcccctggaa gggagggagt agcgtggccg
      121 aaccctggcg cgtgttggtg gtggaagatg atggagccgt gggaaacacc ttggtggcct
      181 tcctggagga cgaaggtttc gacgtgcgcc tggcccgcag cggagaggat gccctgacga
      241 tcctaaaaag tgggtggatc ccccgagtgg gggtcgtgga cctgcgcctc cctggcatgg
      301 acggcagggc ggtggtggag tgcgcccggg tgctagcccc gtcggtgcgt tttctggtgc
      361 acacgggatc ccccgggggc gtggtggaag acttggcctt ctccatggga gtcgacgcga
      421 aagacgtctt catcaagccc gtgcgggact tggggctcct ggtggaacgg ctgcgccgca
      481 gcggcgaagg ggaggatggg catggggcgt gaccccggag gaccaaagca cattctggtg
      541 gtggaagacg acggcgcggt gcgacggcac ctgacggcgt acctggaaga ttgcggttat
      601 cgggtgacgg cggcggcgga gggagaagga gccctggaga tggcccggac ccagcacccc
      661 gacctggtca tcaccgacct gcacatgccc gggatctcgg gctttcaggt cctctcggcc
      721 ctggcggaag agttccccac cctccccgtg ttggtggtct ccggcacgga cctcctgacg
      781 gaggtggtgg aggccctgcg gcgaggggcc tgggactacc tgatcaagcc cttggaagag
      841 atgggggtgt tggagcacgc cgtggagcgg gccctggagc gcgcctggct cctagaagaa
      901 aatcggcgct cccgggagga gctggaagag gccgtggccc ggaggaccca ggagctccag
      961 gccctcctca cggaaaggac ggtcctcctg cgggaggtgc accaccgggt gaaaaataac
     1021 tttcaggtga tatcgagcct cctccagatc cgggcccgaa gctcaagaca tccggaggtg
     1081 cggaactttt cctccgactt tgcccagcgc atccggtcca tggccctggt gcacgacgcc
     1141 ctgtaccaga cctcggactt cgcccggtgg aacttcggag agtacctcaa gcgcctggtg
     1201 ggggacctgg cttggggcga cggctcccgg agcccggagg tgcgggtgga agccgccccc
     1261 ttggaagtgg gcctcgacct ggcggtgccc tgcgccctca ttgcccacga actggtgtcc
     1321 aacgccctgc gccatgcctt tcccgaaggc ggggggcagg taatcgtctc cctgagcgcc
     1381 gaaaccccgg ggatggggca cctggaggtg cgggacgacg gcgtgggcct ttcccaggag
     1441 accctggacc aggccgaagg aggcatgggg ctccagatcg cccgcatcct ggtgcagcag
     1501 ctctcgggca ccctgg
//
LOCUS       DAOOER010000200         1510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_87886_length_1510_cov_7.517526, whole genome shotgun sequence.
ACCESSION   DAOOER010000200 DAOOER010000000
VERSION     DAOOER010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87886_length_1510_cov_7.517526"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="PL162_10570"
     CDS             <1..762
                     /locus_tag="PL162_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013047953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HQF92503.1"
                     /translation="EPLMAELERMKELLNPHEVFLVVDSMAGQEAVAVAESFHKRLAI
                     TGLVLSKLDGDSRGGAALGVRAATGIPVRFAGVGERLDDLELFDAQRMSQRILGMGDV
                     AGLVERMQEATTQEDATRMAESLKKSRLTMDDLLAQLEQMEKMGPLEKVLEMIPGMGK
                     IKDLDSAELDPRHLKRVKAVIQSMTKKERARPEIIKGSRRRRIAEGSGTSVQMVNQVL
                     RQFEQMRELWKRLGSVKSGPHRGGKLRALRNMLPF"
     gene            932..1201
                     /gene="rpsP"
                     /locus_tag="PL162_10575"
     CDS             932..1201
                     /gene="rpsP"
                     /locus_tag="PL162_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HQF92504.1"
                     /translation="MTVRIRLARHGRKKNPAYRLVVADSRSPRDGRFIEMLGTYDPMK
                     DPAEIRVDGDRALEWLREGAQPSETARSLLRRAGVWDRFMEGKTK"
     gene            1217..1465
                     /locus_tag="PL162_10580"
     CDS             1217..1465
                     /locus_tag="PL162_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HQF92505.1"
                     /translation="MASYLPLVEYVVKQLVTEPDAVSVKETPREDGGVSVRISVASGD
                     VGRIIGKKGATINALRQLTRASAAKAGDTVDVDVVEEA"
BASE COUNT          294 a          422 c          553 g          241 t
ORIGIN      
        1 gagcccctca tggcggagct ggagcggatg aaagagctcc tgaaccccca cgaggtcttt
       61 ctcgtggtgg actccatggc ggggcaggaa gccgttgcgg tggcggagtc cttccataag
      121 cgcctggcca tcacgggcct cgttctctct aagctcgacg gcgactcccg ggggggagcg
      181 gccctggggg tccgggccgc cacggggatt cccgtgcgct ttgccggggt gggggagcgg
      241 ctggacgatc tggagctctt cgacgcccag cgcatgtccc aaaggatcct cggcatgggg
      301 gacgtggcgg ggctggtgga gcgcatgcag gaggccacca cccaggaaga cgccacccgc
      361 atggcggaga gcctcaaaaa aagccgcctg accatggacg acctcctggc ccagctggag
      421 cagatggaaa agatgggtcc cctggaaaag gtcctcgaaa tgatccccgg catggggaag
      481 ataaaagacc tggactcggc ggagctggac ccccgacacc tcaagagggt gaaggccgtc
      541 atccagtcca tgacgaaaaa agaaagggcc cggccggaaa tcatcaaggg aagccggcga
      601 cggcgcatcg ccgagggctc gggtaccagc gtccagatgg taaaccaggt gcttcgccag
      661 ttcgaacaga tgcgggagct ctggaaacgc ctggggagcg tgaaatcggg gcctcatcgg
      721 ggaggaaagc tccgggccct caggaacatg ttgccctttt aagctaccat cggggaaggg
      781 gtgccggggt tctggaagtg aagtaggggc ggtggtcccc ggggaaactc gttaaaaagg
      841 cggtgccgcg tttcccccag gggggcgcga tgggattgta gggagacgga gccccaaggg
      901 gcgcgtcatg atgtgatgga ggtgttggag tatgacagtg cgtattcggc ttgcccggca
      961 cgggcgcaag aagaatcccg cttatcgtct ggtggtggcg gattctcgct ctccccggga
     1021 cggacgtttc atcgagatgc tgggcaccta tgaccccatg aaggaccccg cagagatccg
     1081 ggtggatggc gaccgggccc tggagtggct ccgggaaggc gctcagccct cggaaaccgc
     1141 ccgttccctg ctgcggcggg cgggggtgtg ggatcgcttc atggaaggca aaacgaagta
     1201 gacgggaggg agctccatgg ccagttacct gcctctggtg gaatacgtgg tgaagcagtt
     1261 ggtgacggag cccgacgcgg tgtctgtcaa agagacccct cgggaagacg ggggcgtctc
     1321 ggtgcgcatt tcggtggcct ccggcgacgt ggggagaatc attgggaaaa aaggcgccac
     1381 catcaacgcc ctccgccagc ttacccgggc ttcggcggcc aaggccggag acacggtgga
     1441 cgtggacgtg gtggaggagg cctaggtgtc cctgggccgc tccgggacgc cccaccttct
     1501 tccggaggct
//
LOCUS       DAOOER010000201         1502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_88664_length_1502_cov_7.424326, whole genome shotgun sequence.
ACCESSION   DAOOER010000201 DAOOER010000000
VERSION     DAOOER010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88664_length_1502_cov_7.424326"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="PL162_10585"
     CDS             <1..279
                     /locus_tag="PL162_10585"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HQF92506.1"
                     /translation="GMTKERLRADLWLREAAPSVALRTWFHRDRSRWEEFRDRYWAEL
                     DGSPAVGEHLRDLASRGRVTLLFSARDPQHNQAMALRDYLLSSPRKAP"
     gene            358..696
                     /locus_tag="PL162_10590"
     CDS             358..696
                     /locus_tag="PL162_10590"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQF92507.1"
                     /translation="MKTIHAPTAPGSPNPHGVKASMIYNAESAQAVHITLEPGEALKK
                     HITPVDVFFYVLEGTGVVEIGDEKQEVGKDTLIESPAKIPHCWYNESGGVLRILVVKA
                     PRPTESTRLL"
     gene            complement(789..>1502)
                     /locus_tag="PL162_10595"
     CDS             complement(789..>1502)
                     /locus_tag="PL162_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQF92508.1"
                     /translation="ARQIQSFVSQIGGIADQTNLLALNAAIEAARAGEAGRGFAVVAE
                     EVRKLAEESNVAARSIADLAGAITKDLDEVLAASHKNAEESQESSVLAKDTEGKIRKM
                     IEALRAIAGSTQDLAAVSEEQAASSEEIASSVQDMSQKVATGANAAAAAKQDMEEVAH
                     AAEKVAENAEELTHLASDLNGLVAIFRTEESAIPRAALAAAPSRPAASGAVSKPRPAA
                     SGVSPKPRPAAGTTPSRRK"
BASE COUNT          270 a          516 c          469 g          247 t
ORIGIN      
        1 ggcatgacca aggagcgcct tcgggcggat ctgtggctcc gggaggccgc ccccagcgtc
       61 gcccttcgga cctggtttca ccgcgaccga tcccggtggg aagagtttcg ggatcgatat
      121 tgggccgagc tggacgggag ccccgccgtg ggagaacatc ttcgggacct ggcgtcccga
      181 gggcgggtga cgctgctttt ttccgcccgg gacccccagc acaaccaggc gatggccctg
      241 agggattacc tcctttcctc cccccgaaag gccccttagg atcgacggac tccggttcgg
      301 aatctgggac aggacatctt cacatataac aaattcaccc aaggaggatc atgacccatg
      361 aagaccatcc acgcccccac ggcccccgga tcgcccaatc cccacggcgt caaggcgagc
      421 atgatctaca acgcggaaag cgctcaggcg gtgcacatca ccttggaacc cggcgaggcg
      481 ctaaaaaagc acatcacccc ggtggacgtg tttttctacg tgctggaggg aacgggagtg
      541 gtggaaatag gggacgaaaa acaagaggtg ggcaaagaca cgctcatcga aagcccggcg
      601 aagatccccc attgctggta caacgaaagc ggcggcgtgc tccggatcct ggtggtcaag
      661 gccccccgcc ccacagaaag cacccggctg ctctaaagac aaaaggagct acctgaaagc
      721 gaagctaaat cgccccggcc ccggcatatg ccggggccgg ggcgatggat cttgcgcaaa
      781 aaaggaccct acttcctccg agaaggcgtc gtgcccgccg ccgggcgggg ctttggtgag
      841 accccggaag ctgccgggcg gggcttcgac acggctccgg aagccgccgg gcgagacggc
      901 gccgccgcca gggccgcccg ggggatggcg gactcctccg tgcggaagat cgccaccagg
      961 ccgttcagat ccgacgccag gtgggtcaac tcctccgcat tttccgccac cttctccgcc
     1021 gcgtgggcca cctcttccat gtcctgcttg gccgccgccg ccgcattggc ccccgtggcc
     1081 accttctggg acatgtcctg cacagacgag gcgatctctt cgctggaggc cgcctgctcc
     1141 tccgacaccg ccgccaggtc ctgagtggag cccgcgatgg cccggagggc ctcgatcatc
     1201 ttccggatct ttccctcggt gtctttggcc aaaacgctcg attcctggga ttcttcggcg
     1261 ttcttgtggg aggccgcgag gacctcgtcc aggtccttgg tgatggcccc ggccaggtcg
     1321 gcgatgctcc gggcagccac gttcgactcc tcggcaagct tgcgcacctc ctcggccacc
     1381 acggcaaatc cccggcccgc ctccccggcc cgggccgcct caatggcggc gttcaacgcc
     1441 aggaggttgg tctggtccgc aatgcctccg atctgggaca cgaagctctg gatctgccgg
     1501 gc
//
LOCUS       DAOOER010000202         1501 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Synergistaceae bacterium isolate AMR_MDS_4478
            NODE_88744_length_1501_cov_10.055325, whole genome shotgun
            sequence.
ACCESSION   DAOOER010000202 DAOOER010000000
VERSION     DAOOER010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866457
            Sequence Read Archive: SRR5007356
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Synergistaceae bacterium (wastewater metagenome)
  ORGANISM  Synergistaceae bacterium
            Bacteria; Synergistota; Synergistia; Synergistales; Synergistaceae.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 08:06:39
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,120
            CDSs (total)                      :: 2,077
            Genes (coding)                    :: 2,069
            CDSs (with protein)               :: 2,069
            Genes (RNA)                       :: 43
            tRNAs                             :: 41
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 0 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Synergistaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88744_length_1501_cov_10.055325"
                     /isolate="AMR_MDS_4478"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053624"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="37.43 N 138.83 E"
                     /collection_date="2015-02-05"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..1279)
                     /gene="pfp"
                     /locus_tag="PL162_10600"
     CDS             complement(2..1279)
                     /gene="pfp"
                     /locus_tag="PL162_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009200700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HQF92509.1"
                     /translation="MSEASKKTLAVLCGGGPAPGMNGVISALTIAARRADWGVLGVYD
                     GFSRLARGEKEKAAVPLEIGAVSRIHRDGGSVLRTARFNPTKSEEDLRRVVDTLVALG
                     VTHLATIGGDDTAYSASRVAAFAREKLGITLNVVHVPKTIDNDLPLPEGVPTFGFETA
                     RSVGTELVANLMEDARTTGRWYLVVAMGRTAGHLTLGMAKSSGATVALIPEEFRDKPV
                     RLKLVVDILVGSIIKRLAAGKGHGVALVAEGLLDRLNLEDLSSCDSVERDEHGHVRYA
                     EIQFGDILRSEVGKALRELGIKMTLVSKEIGYEVRCAPPVAFDVEYTRNLGYAAFEFL
                     ENGGSGAMITLRDNQVAPLPFEEMLDAATGKTQVRLVKVDSIQYRIARQYMIRIEKRD
                     LEARHRLEQMAMAARLTPDAFLERFSYITDAVY"
BASE COUNT          253 a          536 c          448 g          264 t
ORIGIN      
        1 atcagtacac cgcgtcggtg atgtaggaga aacgctccag gaaggcgtcg ggggtgagac
       61 gggccgccat agccatctgc tccaggcgat gtcgggcttc cagatcgcgt ttttcgatgc
      121 ggatcatgta ctgccgggcg atgcggtact ggatggaatc caccttcacc agccgcacct
      181 gggttttccc cgtggctgcg tccagcatct cctcgaaggg aaggggggcc acctggttgt
      241 cccgcagggt gatcatggcg ccggagcccc cgttttccag gaattcgaag gccgcatagc
      301 ccagattccg ggtgtactcc acgtcaaagg ccacgggagg agcacagcgc acctcgtagc
      361 cgatctcctt gctcaccaaa gtcatcttga tccccagctc ccgcagggct ttgcccacct
      421 cggagcgcag gatgtcgccg aactggatct ctgcgtagcg cacgtggccg tgctcatccc
      481 gctccacgct gtcgcaggag ctcagatcct ccaggttgag ccgatccagc agcccctccg
      541 ccaccaacgc cacgccatgc ccctttcccg ccgccagacg cttgatgatg gaccccacca
      601 ggatgtccac caccagcttg aggcgcacgg gtttgtcccg gaactcctcg ggaatgaggg
      661 ccacggtggc tcccgagctc ttggccatgc ccagggtgag gtgccccgcc gtgcgcccca
      721 tggccaccac cagataccag cgccccgtgg tgcgggcgtc ttccatgagg ttcgccacga
      781 gctccgttcc caccgaccgg gcggtctcga aaccaaaggt gggaacgcct tcgggaaggg
      841 gaaggtcgtt gtcgatggtc ttgggcacgt ggaccacgtt gagggtgatc cccagctttt
      901 cccgggcgaa ggccgccacc cgggaagcgg aataggccgt gtcatccccg ccgatggtgg
      961 ccagatgggt gacccccagg gccaccaagg tgtccaccac ccggcggagg tcctcttcgc
     1021 tcttggtggg gttgaagcgg gccgtccgta gcacgcttcc cccgtcccgg tgaatccggc
     1081 tcacggcgcc gatttccagg ggcaccgccg ccttttcctt ctcgccccgg gccaggcggg
     1141 aaaagccgtc gtacactccc agcacgcccc aatccgcccg ccgggcagcg atggtaaggg
     1201 cactgatgac cccgttcata ccaggagctg gacctccgcc acagagcacc gccaaagtct
     1261 ttttgctcgc ttccgacacc gtggacccct cctccgtcgt cagacctatc caaacaccct
     1321 tgggccccgc acttcctccg ggaccctaac catcgttgcc gggacatctt cctgcaaagc
     1381 cggagggaaa tcacaccccc ccgtccccgg aaacgccggg gacctcggca cgcctcttct
     1441 ttcccccgtc ttgacgggat ggggggtccc aggtcagctc gcggcgccgc cggaccaggt
     1501 c
//
