LOCUS       DAOOIE010000001        19779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_2732_length_19779_cov_3.079142, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000001 DAOOIE010000000
VERSION     DAOOIE010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 19779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19779
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2732_length_19779_cov_3.079142"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..108
                     /gene="rplB"
                     /locus_tag="PK131_00005"
     CDS             <1..108
                     /gene="rplB"
                     /locus_tag="PK131_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010469319.1"
                     /note="one of the primary rRNA-binding proteins; required
                     for association of the 30S and 50S subunits to form the
                     70S ribosome, for tRNA binding and peptide bond formation;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPD44554.1"
                     /translation="GVGMNPKTPWGKPAMGKKTRKKKKQSNKLIIKRRR"
     gene            113..385
                     /gene="rpsS"
                     /locus_tag="PK131_00010"
     CDS             113..385
                     /gene="rpsS"
                     /locus_tag="PK131_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPD44555.1"
                     /translation="MSRSSKKGPFIDKKLFDKIAKNQSGVVRTYCRSSQIAPEFVGHH
                     FEVHNGRKFISVYITEDMVGHRLGEFAPTRTFKGHGRVVKRVIEKT"
     gene            389..760
                     /locus_tag="PK131_00015"
     CDS             389..760
                     /locus_tag="PK131_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012462.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0042255 - ribosome assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uL22 family ribosomal protein"
                     /protein_id="HPD44556.1"
                     /translation="MALKTVVYLDKDLKISPRKLRLLVNEVKRLNPFETVKRLQFVNT
                     KAARMLKLAINNAIADAKNNYHLNPDSLKFKEIRVDEGTKIKRMDRSHGYRFNRGIII
                     KRHSRLIISLTGEETNYGAKS"
     gene            744..1385
                     /gene="rpsC"
                     /locus_tag="PK131_00020"
     CDS             744..1385
                     /gene="rpsC"
                     /locus_tag="PK131_00020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPD44557.1"
                     /translation="MGQKVNPISYRLETGENSPKWQSSWYADGKNYVVFLLEDKKIRD
                     FIQKKLNAAGVIAVRIERSNKKIRVILVVSRPGLVIGRGGKELEVLKKELYKLMAKDH
                     TKLNLEIQVEEFKHADLSAKLIAEKIAFKLAKRMPYRRVVLSAMEKAMLAGAKGIKVI
                     LSGRINGAEISRREKFSQGKVPLSTIRSRIDYAECPSLTRSGYIGVKVYLNLG"
     gene            1393..1794
                     /gene="rplP"
                     /locus_tag="PK131_00025"
     CDS             1393..1794
                     /gene="rplP"
                     /locus_tag="PK131_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPD44558.1"
                     /translation="MLQPGRTKYRKQFRGHMGGISRGNLVAFGDYGLKVTECGWITAN
                     QLEAARRAIAHATRRNGRMWCRIFPDKPITQKGGSHMGAGKGDVKGYVAVVKPGRIIF
                     EVAGVSQEEAKQALRLAAIKLPVTSIIVNRL"
     gene            1791..1994
                     /gene="rpmC"
                     /locus_tag="PK131_00030"
     CDS             1791..1994
                     /gene="rpmC"
                     /locus_tag="PK131_00030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPD44559.1"
                     /translation="MKKTDKIAYRQKTKTELEQLLQEARKKYLESKSKFAIGNLKNTS
                     LFKKMRYEIAFIKTLLKEKKDEK"
     gene            1984..2229
                     /gene="rpsQ"
                     /locus_tag="PK131_00035"
     CDS             1984..2229
                     /gene="rpsQ"
                     /locus_tag="PK131_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008690096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPD44560.1"
                     /translation="MKNKKGKFFTGVVVSDKMEGTIVVELTTARTHPLYKKIIGSNKK
                     VYVENNLKAKIGNRVKIRETRPLSKLKRFTTVAIVKN"
     gene            2238..2609
                     /gene="rplN"
                     /locus_tag="PK131_00040"
     CDS             2238..2609
                     /gene="rplN"
                     /locus_tag="PK131_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPD44561.1"
                     /translation="MSLQLRSIIKAADNCGARSLMIIHVYRGHFHRNATIGDTVMAVV
                     KDAIPNSMVKKKEKVKMVVVRTKKEYGRKDGSYIRFSDNAGVIIDNTGAPRGTRIFGP
                     IAREIKDLGFNKIVSMAKEVY"
     gene            2614..2943
                     /gene="rplX"
                     /locus_tag="PK131_00045"
     CDS             2614..2943
                     /gene="rplX"
                     /locus_tag="PK131_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPD44562.1"
                     /translation="MKILKGDKVKILSGKDKGREGEVIKVFPKKDSVVVKGINLFKKH
                     LRATKDQKGSIIEKEFPLRVAKVALVCPSCQKTTRIGYKIDKSGEKFRFCKKCQALIG
                     LTKTRHD"
     gene            2936..3466
                     /gene="rplE"
                     /locus_tag="PK131_00050"
     CDS             2936..3466
                     /gene="rplE"
                     /locus_tag="PK131_00050"
                     /inference="COORDINATES: protein motif:HMM:NF000585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPD44563.1"
                     /translation="MIDPKEINKQLSELLGKTRNVFSLPRLEKVVVNYRVKEARESSE
                     MLQAAVEEIMAITGQKPKLCRAKKAVSAFKVRKNEPLALKVTLRGKRMYDFVERLFNL
                     VLPRLRDFKGLPTTSFDDQGNYSLIIKDQTYFPEINLNKVNKIRPIQITLNITTASKE
                     EAKMLLSALGCVFEKK"
     gene            3469..3654
                     /locus_tag="PK131_00055"
     CDS             3469..3654
                     /locus_tag="PK131_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HPD44564.1"
                     /translation="MATLAKKVREQKKLKYKVRYHNRCQLCGRPRGYIRFFGVCRLCF
                     RKLTQEGVLPGVKKASW"
     gene            3657..4019
                     /locus_tag="PK131_00060"
     CDS             3657..4019
                     /locus_tag="PK131_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPD44565.1"
                     /translation="MILSLVNDYLIRLKNASQAGKKTVTAPVSKHLVAISELLKKHGF
                     IESYKVEGKTLTAKINRVSEVRFFSTPGRVLYTKSSRIPWGKSRSSLIIISTSKGVMS
                     QRQAAKLHLGGKLIAEIN"
     gene            4039..4578
                     /gene="rplF"
                     /locus_tag="PK131_00065"
     CDS             4039..4578
                     /gene="rplF"
                     /locus_tag="PK131_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013008458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPD44566.1"
                     /translation="MSRIGKQAIIVPNEVKIITKGREIEVIGPKGDLKLHLPPKIEVK
                     IEDNLLLVSRQTDERQSRANHGTIRARLNNMVKGVVVPWEKKLEIRGTGYKAQINGNQ
                     LKVWAGYIKPVEMVLPDGITAVVDEDTKITISGADKEVVGQTASNIRKIRKVEPYKGK
                     GIRYLGEFIKLKAGKKAKV"
     gene            4581..4925
                     /gene="rplR"
                     /locus_tag="PK131_00070"
     CDS             4581..4925
                     /gene="rplR"
                     /locus_tag="PK131_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPD44567.1"
                     /translation="MIIKDHQIRRLKRVRAKLSHNQEIPRLSVFRSNQHLWAQIVDDQ
                     NGRTLVAFSTKNLKETKGTKTEKAFELGKQLAKLALAQNINQVRFDRGLSRYHGRIKA
                     VAEGARQGGLKL"
     gene            4922..5368
                     /gene="rpsE"
                     /locus_tag="PK131_00075"
     CDS             4922..5368
                     /gene="rpsE"
                     /locus_tag="PK131_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018630064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPD44568.1"
                     /translation="MTEEKETSELNNKVIEIKRVSKKTKGGNQIHFTALVVVGDGKGQ
                     VGVGLGKAKSVADAVAKGTKKAQKNLIKVPIVNGTISQVIKIKLKSAVVLLRPGKAGS
                     GIIAGGSVRTVVELAGIKDIVSKIIGSKNKSLNVWATFKALGCLQE"
     gene            5365..5757
                     /locus_tag="PK131_00080"
     CDS             5365..5757
                     /locus_tag="PK131_00080"
                     /inference="COORDINATES: protein motif:HMM:NF013024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uL15 family ribosomal protein"
                     /protein_id="HPD44569.1"
                     /translation="MNLILSHLEKITNKPARRLGRGYGSKKGGHTSGRGQKGDKSRGK
                     VPLIFDGTKVKKGYIKRLPFLRGKHRLGVKTKPQAVSLDYLEAHFKEGETVKFDFPVK
                     ILANGKIKKKLVLKDIPMSASARKKINE"
     gene            5750..7027
                     /gene="secY"
                     /locus_tag="PK131_00085"
     CDS             5750..7027
                     /gene="secY"
                     /locus_tag="PK131_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPD44570.1"
                     /translation="MNKLIHSLSVYKEAITIPVLRQKILFTLAMLVIFRLIAHIPVPG
                     VNLALMQKFFNQNQILALVNMFSGGTLANFSIVALGLNPYINASVMMQLLALVIPQLA
                     ELQKEGEYGRARINQYIRVATIPIALIQSIGMYSLLRSQQIIISLSPLSLIALAISMV
                     SGTMIMVFLGEMINLYGVGNGISMMIFGSIISHLPVSFYQTFSLTDLSNSQNVFNIFI
                     YLALALIIILGVVFVEEAVLRVPVHYARRGQTTFLPIKVDTAGVMPIIFAVSLATIPS
                     LLGQFLSRIPGGVGQFGTFLARTFRADSSVYVIFYFLLVVAFTFFYTSVIFKPKDIAD
                     ELRKTGAFIPGIRPGVSTQTRLQYLITRVIAIGAVFLGLIAIVPSLLQKATGLTTLSI
                     SGTGILIIVSVVIELTRKIENVVQSYNYEKLTY"
     gene            7031..7633
                     /locus_tag="PK131_00090"
     CDS             7031..7633
                     /locus_tag="PK131_00090"
                     /inference="COORDINATES: protein motif:HMM:NF012624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HPD44571.1"
                     /translation="MNLFIIGPSGCGKSTQAKLIAEKYGLTHFSMGQLLRDEMAEQSV
                     LGLEAKVYVDQGRWVPNPLIYRILTAKLETVNNQNFIIDGFPRHFDQGVMMEQYLTSK
                     QQAIDAIIHLDVTFEEIIKRRTIQAEAGHRFSNVGRTDETPASIAARQKSYEETINPI
                     LDYFQSKNKLWRIEGNRPIDPIFADICSLINTKLNLLVEK"
     gene            7630..7836
                     /gene="infA"
                     /locus_tag="PK131_00095"
     CDS             7630..7836
                     /gene="infA"
                     /locus_tag="PK131_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPD44572.1"
                     /translation="MMKEPTIKGVVVEVLPDALFRVRLENGNVIIAYAAGRLKMNHIR
                     CLAGDTVSLVLSPDGKKGRIVYRG"
     gene            7893..8006
                     /gene="rpmJ"
                     /locus_tag="PK131_00100"
     CDS             7893..8006
                     /gene="rpmJ"
                     /locus_tag="PK131_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006250018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPD44573.1"
                     /translation="MKVKSSVKRRCRSCQVVIRRGVRRVICPIKKHSQRQG"
     gene            8009..8464
                     /gene="rpsM"
                     /locus_tag="PK131_00105"
     CDS             8009..8464
                     /gene="rpsM"
                     /locus_tag="PK131_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPD44574.1"
                     /translation="MMRILGVEIPDNERAEVGLTSFYGIGPAMARELLALTHISVDKR
                     IKDLTREEVADLMKVLENYKIEGDLRKEIRENIERLKAIRSYRGLRHLIGLPVRGQRT
                     RSNARTRKGKRKTVGSLTKEAWAKIEAAQQKEKGETVPSVTPTDETSKQ"
     gene            8464..8850
                     /gene="rpsK"
                     /locus_tag="PK131_00110"
     CDS             8464..8850
                     /gene="rpsK"
                     /locus_tag="PK131_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPD44575.1"
                     /translation="MTGEKIKKKVIKSFAEGRLYVYSSFNNTIVTVTDPKGNVLSWSS
                     AGHCGFKGAKKATPFAASIALEKALGAVKPMGIKRLWVYLKGPGVGRDATLRFLRAKK
                     DFEIEQIADVTPLPHNGPRPPKRRRI"
     gene            8853..9485
                     /gene="rpsD"
                     /locus_tag="PK131_00115"
     CDS             8853..9485
                     /gene="rpsD"
                     /locus_tag="PK131_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPD44576.1"
                     /translation="MSRITVNSKCRLCRAEGQKLYLKGSRCDGAKCPIEKKGAVQPGM
                     HGVKRRSKISAYGLQLRAKQKAKRLYGVAEAQFKNYYLKAKKLKGLVGDNLQILLERR
                     LDNVVYRAGLSSSRSQAKQFISHRHILVNNKKVNINSYQIKPGDIIAVDKLTSDGFGE
                     SLPVAAKDFKPLNWLKVDKNKLMVEVTSLPTKEDLPTDIDLNLIIEYYSR"
     gene            9490..10413
                     /locus_tag="PK131_00120"
     CDS             9490..10413
                     /locus_tag="PK131_00120"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPD44577.1"
                     /translation="MIDTSKFNVKTVKQTKDYGKFEMGPLPMGFGHTLGNSLRRVLFI
                     TIPGAAITRIKVEGANHLFTSLPGIKEDLVEISLNLKKVKFIYKSEKPIVLYLNKKGM
                     GPVTAADIEDSDLCRVVNKDQFIATITDKKTSLKMELTVESGVGYTMAREQKTDIVGE
                     IILDATFTPVTKVNYTVEPTRLGKKTNYDKLTVEIWTDGSIEPRTALKYSAKDLVAAF
                     NQIVEPREFSEEVVTLSANPAATMGIDMSVEEIELPLRVINALKKAGFSTIDNLVKAG
                     RSEVSRARNVGEKSLKIIDIWLKDKGFGWKQ"
     gene            10414..10935
                     /locus_tag="PK131_00125"
     CDS             10414..10935
                     /locus_tag="PK131_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L17 family ribosomal protein"
                     /protein_id="HPD44578.1"
                     /translation="MKHRISGKKLGRNTNQRKALWLNQVRSMFIYGKIKTTSAKSKSV
                     LPVIEKLGTVIMTQPDLIARRALFRYLQDRTWVNNVYDQFRKVFDGRTANFTKITRIK
                     RRWGDDALIVELSFIKPIAFSQSKMTKKVAMTAPTSANSQVKPIANRSVKKKNETKKE
                     VKDETKNKRSQRK"
     gene            10901..11329
                     /gene="rplM"
                     /locus_tag="PK131_00130"
     CDS             10901..11329
                     /gene="rplM"
                     /locus_tag="PK131_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPD44579.1"
                     /translation="MKPKTSVLRGNKANRKWHLIDLDGKILGRVSTEIASLLMGKNKP
                     TFSYHRDDGDYVVCVNAAKIQVTGKKLRQKSHFWHTPWPGHLKSLTWEALLKKDPRQL
                     IYLSVANMLPKNRLRDSRLKRLKVFTGLNHPYGDKFKKDN"
     gene            11301..11717
                     /gene="rpsI"
                     /locus_tag="PK131_00135"
     CDS             11301..11717
                     /gene="rpsI"
                     /locus_tag="PK131_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPD44580.1"
                     /translation="MATNLKKTTKTYTYAIGRRKCAVAAIKLFKGKGEMTVNKSAIDK
                     YFPLSSDKIIYERPLVVTNTLGKYYYEARANGGGKHGQLEAVTLAVARALKKVNDKFG
                     PILRTNGLLTVDSRIRERRMVGTGGKSRRMKQSPRR"
     gene            11749..12462
                     /locus_tag="PK131_00140"
     CDS             11749..12462
                     /locus_tag="PK131_00140"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPD44581.1"
                     /translation="MSYLVLAIVQGITEFLPISSTGHLNLYQHFFHLTPSLSLDIFLH
                     GGTLIAVLFVFRKQFRYFWKNFWYIIMASIPAGIIGILFKEQFDTLFANVKLLPFFFL
                     ITTMFLFLTKNLKTNQSRLTYKNALIIGIFQAIALLPGVSRSGATIAAGLLVGLPPLE
                     AFNFSFALFIPATLGAIVLAYKDIVFFPSLIIPTILTIITGILALNFLKKTLISKNFW
                     KFSLYTFILALILLLVLNF"
     gene            complement(12469..13422)
                     /locus_tag="PK131_00145"
     CDS             complement(12469..13422)
                     /locus_tag="PK131_00145"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HPD44582.1"
                     /translation="MISQLSANQKINEEHFLVKKVDKKTARNGNNYYDLELNDSTGVI
                     IGRIWDNALPACNFEIGKVIEVSGQTDEYNGKLSLIINACQIVNGEDIANFKTVIPTM
                     VFDLETVGDKFEELDQQQQDYLLNTLEKYEEDKEKSKKRTALYAIFGKICAAGCYNPD
                     KKQGITIVIGEKKLCPEKNNFTYIVVKNEKELLEKFWELASKVQRFVTFNGNNFDIPY
                     LIIRSGINRVKIPFEIKKYSQNFIDLEDRLKQNGKPFKLEMLCRVFGIDDPKDKGVTG
                     AEVSNLFYQKKFQQIADYVTRDAVATAELYQIWREYLSGEI"
     gene            13571..13644
                     /locus_tag="PK131_00150"
     tRNA            13571..13644
                     /locus_tag="PK131_00150"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13605..13607,aa:Pro,seq:ggg)
     gene            13757..14482
                     /locus_tag="PK131_00155"
     CDS             13757..14482
                     /locus_tag="PK131_00155"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HPD44583.1"
                     /translation="MDQKQIIKQIRKELKNKASTKTDKEREKAKALFGNETKLLALRV
                     PETRKIAESFGKELKKEKDFDTALKVADQLYKSGVFEEATVAESILKEFKNYFDNLTF
                     DKFDEWIDYIKNWANIDMLCGWLITPIILKDDTKLKTILRWTKSKNRWRRRAAIVSFV
                     KPVKKGKYLKQALEVAEELIQDEDEMVQKGVGWLLKEAGGVFPDETTRFLMRWRPKIS
                     KLVLRLAREKLPLDKIFYIKKVE"
     gene            14567..14689
                     /locus_tag="PK131_00160"
     CDS             14567..14689
                     /locus_tag="PK131_00160"
                     /inference="COORDINATES: protein motif:HMM:NF024086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPD44584.1"
                     /translation="MPAPKPENFGTNADGSKSEEYCQYCFQSGKFTEPNITMGR"
     gene            14823..15284
                     /locus_tag="PK131_00165"
     CDS             14823..15284
                     /locus_tag="PK131_00165"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPD44585.1"
                     /translation="MAEEIKKDKKFIGRLIAYFESASASEQGHLIESLEYASQDKPEF
                     VAPYFNFVLEHLADKAPRVKWEAARIIANLAEKYADKTEKAIPELLKNSSDKGTVVRW
                     SAAFALGEIAKSNSNAQKTLLPKMKELVMKETNNGVKNVYLKALKVIEKEK"
     gene            complement(15408..16157)
                     /locus_tag="PK131_00170"
     CDS             complement(15408..16157)
                     /locus_tag="PK131_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPD44586.1"
                     /translation="MRLENKIAIVTGAGSGIGREIALAFAREGAKLIIADLSVEGGQA
                     TEAQIKQAGGEALFVKTDVSQATEIEQMVKTCLDQFSRVDILVNNAGIYRTFNLHEMT
                     EAEWDQTINVNLKSVFLGSRAVLPQMLKQGQGKIINTASIAGLVGFAQSGAYCATKGG
                     IIALTREMALDYAPQKINVNCICPGVIKTAMTKEMTTDPNSKQFVESSTPYPRLGEPA
                     DIAAAAVYLASDEADFVNGAVLVVDGGWTSR"
     gene            16251..17321
                     /gene="recA"
                     /locus_tag="PK131_00175"
     CDS             16251..17321
                     /gene="recA"
                     /locus_tag="PK131_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPD44587.1"
                     /translation="MKKKAKKEKVTASGDLADKKLEAVKLAMEQITKAYGAGAIMRMG
                     EQAGLGQVEVIPTGCLPLDVALGVGGLPRGRVTEIFGPEASGKTTLALRVVAEAQKMG
                     GTAAFIDAEHALDPARAAVIGVDLNTLLISQPDNGEQALEIVETLVRSNAVDVIVVDS
                     VAALVPKAEIEGEMGDAMMGVQARLMSQALRKLTGAISKSRAVVIFTNQIRQKIGVIF
                     GNPETTPGGLALKFYSSVRIDVRKIANIKTAAGEPVGSQHRARIVKNKVAPPFKECEF
                     DITNNEGLSIYGSLVDLAVEQGLIEKSGAFFKFNSQSIQGREGMKKYLQDHPEAVAKL
                     RDDIWSKVKADNEIAKSQTVKN"
     gene            17287..17820
                     /locus_tag="PK131_00180"
     CDS             17287..17820
                     /locus_tag="PK131_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44588.1"
                     /translation="MKLQSLKPSRIKNRVNLVFDDGSYLPFLVDEALKLGLKTGQEVN
                     FMELKEKSTNYLAREYALRQIALSPKTEKLLRRKLLHKFPNFLPDQLIQELKPYLEET
                     QYINYILKRFKDRSNREISYRLKLAGINYQSQENDLAKIKALLKKKPKTTFASLIRKG
                     FAYDDVKSTFDKLTSIE"
     gene            17941..19395
                     /locus_tag="PK131_00185"
     CDS             17941..19395
                     /locus_tag="PK131_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HPD44589.1"
                     /translation="MFDSFIKTFKNLLTPTGLETPKESNKVEYTKVKPEVKTEVVSAK
                     ENLAEAELLRRLALLDEKEKYLIRKEKMLDSRNAQIQAKLAQIDDVYRKQLEKLEQIS
                     GLDQEKAKQLIISSTEKRMANWVAKKIEEAKELIKQREDEEAKEILTEALKHGVTDTV
                     AEYTVSTITLADEKIKGKVIGREGRNIHAFEKATGVELELDEGNDIRISSFDSTRREI
                     ARLALEKLIRDGRIQPLRIEQVVNQTKQEMDKILLAEGKRICQEVGVFNLPIDLMKEI
                     GKYKFRFSYGQNLAKHSVEATKIAVDLAYELKANVGVVRLGALLHDIGKVISDQEGTH
                     VELGVNLLHQYKLPEAVINCIAEHHEGKEFSSIESVIVYLGDAASGVRPGARYEVHEE
                     YLKRMQNIEEIAKQFNGVQSVAAYQAGREVMVIVDPGMVSDAEAEVLSQNIAERLDEE
                     AKWAGQIRVTVIRELKASSIVSGNKISKNAGKNE"
     gene            19458..19730
                     /locus_tag="PK131_00190"
     CDS             19458..19730
                     /locus_tag="PK131_00190"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPD44590.1"
                     /translation="MTKKDLIEVVAQKAKLSKKAAKQAVETFLKEIMKALSAGEVVKL
                     SGFGTFKTKMFKAKTIKAIGSKEAKKIVARRMPRYIPGTKIKKMVR"
BASE COUNT         6394 a         3210 c         4217 g         5958 t
ORIGIN      
        1 ggggtaggaa tgaatcctaa aactccttgg ggtaaaccag ccatgggtaa aaaaactcgc
       61 aagaagaaaa agcaatccaa taaattaatt attaagagaa ggcggtaatt taatgtcaag
      121 aagttctaaa aaaggtccat ttattgataa aaaattattt gataaaatag ccaaaaatca
      181 atcaggtgtt gttcggactt attgccggtc atctcaaatt gctccggagt ttgttggtca
      241 tcatttcgag gttcataatg gtcgaaagtt tatttccgtt tatataactg aagatatggt
      301 tggtcaccgg ttgggtgaat ttgctccaac ccggacattt aaaggtcatg gccgagtagt
      361 taaaagagta attgagaaaa cttaaactat ggctcttaaa actgttgttt atctcgataa
      421 agatttaaag atttcgccgc gaaagttacg gttattagtg aatgaggtaa aaagactaaa
      481 tccttttgaa acagtaaaac gacttcagtt cgtcaatact aaagccgccc gaatgctaaa
      541 attggcaata aataacgcca ttgctgacgc taaaaataat tatcatctca accctgattc
      601 attaaagttt aaagaaataa gagtggacga ggggactaaa ataaaaagaa tggatcgttc
      661 ccatggctac cgttttaatc ggggaattat tattaaacgt catagccgtt taattattag
      721 tttaactggc gaagaaacaa attatggggc aaaaagttaa tccgatttct tatcgattag
      781 aaacgggtga aaatagtcct aaatggcaat cgtcatggta tgctgacggt aaaaattacg
      841 ttgtcttttt attggaagat aaaaaaatcc gtgattttat tcaaaagaaa ctaaatgcag
      901 ctggtgttat agccgtgaga attgaacgtt ctaataaaaa aatcagagtt attcttgtgg
      961 ttagtcgtcc gggtttggtt attggtcggg gcggtaaaga attggaggtt ttaaaaaagg
     1021 aattatataa gttaatggct aaagatcaca ctaaattaaa tttagaaatt caggtagagg
     1081 aatttaagca tgctgattta tcagccaaat tgattgcaga aaaaattgct tttaaattag
     1141 ctaaaagaat gccttaccga cgggtggttc tttcagcgat ggaaaaggcg atgttagctg
     1201 gcgccaaagg aattaaagtt attttaagcg gccggattaa tggagctgag atcagccgtc
     1261 gagaaaaatt ttcccaaggt aaagtaccct tgtcaactat tagaagtcgg attgattatg
     1321 ctgaatgtcc ttctttaacc cgctctggtt atattggagt taaagtttat ttaaatttag
     1381 gttaataatt ttatgctgca accaggtcga actaaatatc gaaaacaatt tcgaggtcat
     1441 atgggcggta ttagccgggg aaatttagtt gcctttggtg attatggttt aaaagttact
     1501 gagtgtggtt ggataacggc taatcaatta gaggcggccc gccgggctat tgctcatgcg
     1561 accagacgaa atggtcgaat gtggtgtcgt atttttccag ataagccaat aactcaaaaa
     1621 ggcggcagtc atatgggggc aggcaagggt gatgttaaag gttatgtggc ggtggttaaa
     1681 cctggtcgga ttatttttga agtagctggt gttagtcagg aagaagctaa acaggccctg
     1741 cgtttagccg ccattaaatt accagtgact tcaattattg ttaaccgttt atgaaaaaaa
     1801 ctgataaaat tgcctatcgg caaaaaacca aaactgaatt ggagcaattg cttcaggaag
     1861 ccagaaaaaa atatctagaa agcaagtcga aatttgcgat aggtaatctt aaaaatacct
     1921 ctctttttaa aaaaatgagg tatgaaattg cttttatcaa aactttatta aaagaaaaaa
     1981 aagatgaaaa ataaaaaagg gaaatttttt acgggagtag ttgtcagtga taagatggaa
     2041 ggaacaatag tggtagagct gactacagct cggactcatc ccctatataa aaaaattata
     2101 gggagtaata aaaaggttta tgtagaaaat aatttaaaag ctaaaattgg aaatagagtt
     2161 aaaattcgtg aaactagacc tttatctaaa ttaaaacgtt ttactaccgt agctatagtt
     2221 aaaaattaag aacacaaatg agtttacaat taagatcaat cattaaagcc gctgacaatt
     2281 gtggcgcccg atctttgatg attatccatg tttatcgtgg tcattttcac cgaaatgcta
     2341 ctataggtga tacggtaatg gcagtagtta aggacgctat tcccaatagt atggttaaaa
     2401 agaaagaaaa ggtaaaaatg gtcgtggtta gaaccaaaaa ggaatatgga cggaaggatg
     2461 gttcttatat ccgcttttcc gacaatgccg gagtaattat tgataacacc ggtgctcctc
     2521 ggggtacccg catttttgga cccattgcta gagaaattaa agatttgggc tttaataaga
     2581 ttgtttcaat ggccaaggag gtttattaat ctaatgaaaa ttttaaaggg tgataaggta
     2641 aaaattttgt ctggtaaaga taagggccgt gaaggcgagg taatcaaggt atttcctaaa
     2701 aaagattcag ttgtagtgaa aggaattaat ttgtttaaga aacatcttag agcgactaaa
     2761 gatcaaaagg gtagtattat cgaaaaagaa tttcccttga gagttgctaa agtggccttg
     2821 gtttgtcctt cctgtcaaaa aacaactcgg ataggttata aaattgataa gtctggggaa
     2881 aaattccgat tttgtaaaaa atgccaagct cttattggtt taactaaaac ccgccatgat
     2941 tgacccaaaa gaaattaata aacaattgtc ggaattgttg ggaaaaacga gaaatgtttt
     3001 ttctttaccg cggcttgaga aggtggtggt taattaccgg gttaaggaag ctcgagagtc
     3061 atcggaaatg ctccaagccg ctgtcgagga gataatggct attaccggtc agaaacccaa
     3121 actttgtcga gctaaaaaag cagtctcagc ctttaaggtg agaaaaaatg aaccgttggc
     3181 tttaaaagta accctgcggg gaaagagaat gtatgatttt gttgaaagat tatttaattt
     3241 agttttaccg cgtttacgcg atttcaaagg attgccaact acgtcgtttg atgatcaagg
     3301 taattatagt ttaattatta aagatcagac ttattttccg gaaattaatt taaataaggt
     3361 taataaaatc agaccaatcc aaattaccct gaatataacc actgcttcca aagaagaggc
     3421 aaaaatgtta ttatctgccc taggttgcgt ttttgagaaa aaataaatat ggctacttta
     3481 gcaaaaaaag ttagagaaca aaaaaaatta aaatataagg tgaggtatca caatcggtgc
     3541 cagctttgcg gtcggccccg aggttatatt cgtttttttg gtgtttgtcg tctttgtttt
     3601 agaaagttaa cccaagaagg ggttctgcca ggagtaaaaa aggcgagttg gtaaaaatga
     3661 tcttatcttt agtcaacgat tatctgattc ggcttaaaaa tgcctcacag gcaggaaaaa
     3721 aaacagttac tgctcctgtt tcgaagcatt tagtggccat ttctgaatta cttaaaaaac
     3781 acggttttat tgaaagttat aaggttgaag gaaaaacttt gacggcaaag atcaatcggg
     3841 ttagtgaagt tagatttttt tctaccccag ggcgagttct ttatactaaa tccagccgta
     3901 taccgtgggg taagagtcgg tcttcgttaa ttattatttc cacttccaag ggagttatgt
     3961 ctcaacgtca ggcagctaaa cttcatcttg gtggtaaatt aattgctgaa attaattaaa
     4021 aattaaaaat ttcaagaaat gagtcgaatt ggtaagcaag ctattattgt tccgaatgaa
     4081 gtaaaaatca taacgaaagg gagagaaata gaggtgatag gacctaaggg tgatttaaaa
     4141 ttacatcttc ctcctaaaat tgaagttaaa atagaggaca atcttctttt ggttagtcgt
     4201 caaaccgatg agcggcagtc tcgagctaat catggaacaa ttcgggcccg attaaataat
     4261 atggttaaag gagtagtggt tccttgggag aaaaaactgg agattcgagg tactggttat
     4321 aaagctcaga ttaacggtaa tcagttaaaa gtttgggctg gttatattaa gccggtcgaa
     4381 atggttttac ctgatgggat taccgccgtg gttgatgaag atactaaaat tactattagt
     4441 ggtgctgata aagaagtggt tggtcaaaca gctagtaata tccgaaaaat tagaaaagtc
     4501 gagccttata aaggtaaggg aattcgttat ttaggtgaat ttattaagtt aaaggctggt
     4561 aaaaaggcaa aagtttaact atgataatta aagatcatca aattcgtcgt cttaaaagag
     4621 ttcgggctaa actgagtcat aatcaggaaa tacctcgctt gagtgttttc agaagtaatc
     4681 agcatctttg ggcccagatt gttgacgatc aaaatggccg aactttagtt gctttttcaa
     4741 ctaaaaacct taaggaaact aaggggacta aaacagaaaa agcgtttgag ttaggtaaac
     4801 aattggctaa attggcctta gcacaaaata tcaaccaagt tagatttgat cgtggtctaa
     4861 gccgttatca tggccggatt aaagccgttg ccgagggggc tagacaagga ggtttaaaac
     4921 tatgacagaa gaaaaagaaa cgtccgaatt aaataataag gtaatagaaa taaaacgggt
     4981 ttccaagaag actaagggtg gtaatcaaat ccattttacg gccttagtgg tagtaggtga
     5041 tggcaagggt caagtaggag ttggtttagg gaaagctaag tcggtagctg atgccgtagc
     5101 taaaggaact aagaaagccc agaaaaattt gattaaggtt cccattgtta atggtactat
     5161 ttctcaggta attaaaatta aacttaaaag tgcggtggtt ttgcttcgtc caggaaaagc
     5221 tggcagcggc attattgctg gcggatcagt gagaacagtg gttgaattgg ccggaattaa
     5281 ggatatagta agtaagatta ttggttctaa gaataagtcg ttaaatgttt gggctacctt
     5341 caaagccctt ggttgtttac aagaatgaac ttaattttgt cacatttaga aaaaattact
     5401 aataaaccag ctcggcgttt aggacgggga tatggctcaa aaaagggagg tcataccagt
     5461 ggccgtggtc aaaaaggtga taaatctaga ggaaaagttc cccttatttt tgacgggact
     5521 aaagttaaaa agggttatat taaaagactg ccgtttttgc gcggtaaaca ccgtcttgga
     5581 gtcaagacta aacctcaggc agttagtctt gactatttag aagcccattt taaggaagga
     5641 gaaacagtta aatttgattt tccggtaaaa attttagcta atggtaaaat taagaagaaa
     5701 ttggttttaa aagatatacc aatgtcagcc tcggctagaa aaaaaataaa tgaataagct
     5761 gatccactct ctttcagttt ataaagaagc gataacaatt cctgttttac gtcaaaagat
     5821 tttgtttact ttagcaatgt tggtaatctt tcgtttaatt gctcatattc ccgttccggg
     5881 agttaatttg gctttaatgc aaaaattttt taatcagaat caaattttag ctttggtgaa
     5941 tatgttttcc ggcggtactt tagctaattt ttctatcgtt gccctgggtc ttaatccata
     6001 tatcaatgct tcagtaatga tgcaattatt ggctttagtg attcctcaat tagccgagtt
     6061 gcaaaaggaa ggagaatatg gccgggctcg aattaatcaa tatattcgtg tcgctactat
     6121 tccgattgcc ttaattcaat caattgggat gtacagttta cttcgttccc agcaaattat
     6181 tatttctttg tcaccattat ctttaatagc tctggcgatc agtatggtaa gcggcaccat
     6241 gattatggtg tttttgggag aaatgattaa tctttacgga gttggcaacg gtatttccat
     6301 gatgattttt ggtagtatta tttctcattt accggtaagt ttctatcaga ctttttcttt
     6361 aactgattta tccaattctc aaaatgtttt taatatattt atttatctag ctctagcatt
     6421 aataatcatt ttaggagtag tttttgttga agaggcggtt ttacgggtgc cggttcatta
     6481 tgcccgaaga gggcagacta cttttttacc aattaaagtt gacacggcgg gagtaatgcc
     6541 gattatcttt gctgtttctt tagccacaat tccgtctctg ttgggacagt ttttaagccg
     6601 gatcccaggt ggtgtcggtc agtttggaac ttttttagcc agaacttttc gggccgatag
     6661 ttctgtttat gtgatttttt attttctttt ggtagttgct tttacctttt tttatacctc
     6721 agttattttt aaacccaagg atattgccga tgaactgaga aaaaccggag cttttattcc
     6781 cggcattcgt cctggtgttt ctactcaaac ccggctccag tatttaatta ccagggttat
     6841 tgccattggg gctgtttttt taggtttaat cgccattgtt ccttcattac ttcagaaagc
     6901 aactggactc accacccttt ctatcagtgg cactggtatt ttaattatcg tttcggtggt
     6961 gattgagtta acccgtaaga ttgaaaatgt tgtccaaagt tataattatg aaaaattaac
     7021 ctattaattt atgaatttat ttattattgg tccctctggt tgcggcaagt caactcaggc
     7081 taaattgatt gctgaaaaat atggtttgac ccatttttcc atgggccaat tattaagaga
     7141 tgaaatggct gagcaatcag ttcttggttt ggaagctaag gtttatgtag atcaaggtcg
     7201 atgggtacct aatcctctaa tttaccgcat attaactgca aaattggaga ccgttaataa
     7261 tcaaaacttt attattgatg gttttcctcg tcattttgat caaggagtta tgatggagca
     7321 atatttaact agtaaacagc aagcaataga tgcaattatc caccttgatg ttacttttga
     7381 agagataatc aagagacgaa caattcaagc cgaggcaggt catcgctttt ccaatgttgg
     7441 ccgcaccgat gaaaccccag ccagtattgc tgctaggcag aaatcatacg aagaaactat
     7501 taatcccatc cttgattatt ttcagtcaaa aaacaaactt tggagaattg aaggtaatcg
     7561 tccgattgat ccaatttttg ctgatatttg ttccttaatt aataccaaac ttaatttatt
     7621 agttgaaaaa taatgaaaga accaactata aaaggggtag tagttgaggt tttacctgat
     7681 gccttatttc gagttcgact cgaaaatggc aatgtgatta ttgcctatgc agcaggtaga
     7741 cttaaaatga accatatccg ctgtttggcc ggcgataccg tttctctagt tttaagtccc
     7801 gacggtaaaa aaggtcgaat tgtctaccgt ggttaattaa atttactctt tgatattcag
     7861 ccgattttag gttacaatat ccaagttatt ttatgaaagt taagtcgagt gttaaacgtc
     7921 gatgtcgcag ttgtcaggtg gttattcgcc gaggagtgcg gcgggttatt tgtcctatta
     7981 aaaaacattc ccaaagacag gggtaattat aatgagaatt ttaggagttg aaataccaga
     8041 caacgaaaga gctgaggttg gtttaaccag tttttatggc attggcccgg ctatggcccg
     8101 agaattatta gcccttaccc atatttccgt tgataagcgg attaaagatt tgactcggga
     8161 agaagtggct gatctaatga aagttttgga aaattataaa attgaagggg atttaaggaa
     8221 ggagataaga gaaaatattg aacgattgaa agcaattaga agttaccgcg gtttacgtca
     8281 tttgataggt ttgccggtcc gtggccaacg aaccagatcc aatgcccgga ctcgtaaagg
     8341 caagcgaaag acggttggtt cgttaactaa ggaagcgtgg gctaagattg aagccgccca
     8401 acaaaaagaa aaaggggaaa cggttccctc cgtaacgcca acggatgaaa ccagtaaaca
     8461 ataatgacag gagaaaaaat taaaaagaaa gtaatcaaaa gttttgccga aggacggctc
     8521 tatgtttact cttcttttaa caacactatt gttacggtta ctgatcccaa aggcaatgtt
     8581 ttaagctggt catcggccgg ccattgtggt tttaaggggg ctaagaaggc cactcccttt
     8641 gctgcttcga tcgctttaga aaaagctttg ggggcagtaa agccgatggg gattaaaaga
     8701 ttatgggttt atttaaaagg accaggggtt ggtcgtgatg caactcttcg ttttttgaga
     8761 gctaaaaaag attttgaaat agagcaaatt gccgatgtta ccccattacc tcataatggc
     8821 ccccggccgc ctaaaaggag gagaatttaa ttatgtcgag gattacggtt aattcaaagt
     8881 gtcgtttatg ccgggctgag ggacagaaac tttatttgaa agggagccgt tgtgatggtg
     8941 ctaaatgtcc aattgaaaag aaaggagcag ttcaaccggg gatgcatgga gtcaaaagaa
     9001 ggtcaaaaat ctcggcctat ggtttgcagt tgcgagctaa acaaaaggct aaacgtcttt
     9061 atggtgtcgc tgaggctcaa ttcaaaaatt attatcttaa agctaaaaaa cttaaaggct
     9121 tggttggtga taatttacag attcttttag aacgccgctt agataatgta gtttatcggg
     9181 ctggtttaag ttcctcccgt tctcaggcaa aacaatttat cagtcataga catattttag
     9241 ttaacaacaa aaaggttaat attaattctt atcagattaa accaggagat ataattgctg
     9301 tagataaact gactagcgac ggatttggtg aatctctccc tgttgctgcc aaagatttta
     9361 agccgttaaa ttggcttaaa gtagataaaa ataaattaat ggtcgaggta acctctttac
     9421 cgactaaaga ggatctaccc accgacattg atcttaattt aattattgaa tattattcaa
     9481 ggtaaaacta tgattgatac aagcaaattt aatgttaaaa ccgtaaaaca aaccaaagat
     9541 tacggcaagt ttgaaatggg tccattacca atggggtttg gccatacgtt gggtaattct
     9601 cttcgaagag ttttgtttat aacaattccg ggagcagcta ttacccgaat taaggtagag
     9661 ggagccaacc atttatttac cagtttgcca ggaattaaag aagatttggt agaaatatct
     9721 ttgaatctta aaaaagtaaa gtttatttat aaatcagaga aaccaatagt tctttattta
     9781 aataaaaaag gaatgggacc ggtaactgcc gccgatattg aagacagtga tttgtgccgt
     9841 gttgtcaaca aagatcaatt tattgctact attactgata aaaagacatc gttaaaaatg
     9901 gagttgaccg tagagtcagg agtaggttat accatggccc gggagcagaa aactgatatt
     9961 gtcggcgaga ttattttaga tgctaccttt acgccggtaa ctaaagttaa ttatacggtt
    10021 gagccgactc gattaggcaa aaaaactaat tatgataaat taacggtaga aatttggact
    10081 gacggttcga ttgaaccaag gacagctttg aaatattcgg ctaaagattt ggttgctgct
    10141 tttaatcaga ttgtggagcc aagggaattt agtgaagaag tggtaacttt atcagctaat
    10201 cccgccgcga cgatgggaat tgatatgtca gtggaggaaa ttgaattacc tttgagggtt
    10261 atcaatgccc ttaaaaaggc tggttttagt acgattgata atttggttaa agctggtcga
    10321 tctgaagtaa gtagagctag aaatgttggt gaaaaatcgt taaaaattat tgatatctgg
    10381 ctgaaagata aaggttttgg ctggaagcag taaatgaaac accgaatttc gggtaaaaaa
    10441 ttaggtcgca atactaatca gaggaaggcc ttgtggctta atcaggtaag gagtatgttt
    10501 atttacggca agattaaaac cacttcggcc aaatccaagt cggttttacc ggtgatagaa
    10561 aaattaggga cagtaattat gactcagcca gatttaattg cccgacgggc tctctttcgc
    10621 tatttgcaag atcggacttg ggttaataat gtttatgatc agtttcgaaa ggtctttgac
    10681 ggtcgaacag ctaattttac caaaattacg agaattaaac ggcgctgggg cgatgatgct
    10741 ttaatagtag agttaagttt tattaagccg atagctttta gtcaaagtaa aatgactaaa
    10801 aaggtggcaa tgacagcacc aacatccgct aattcgcaag ttaaaccgat tgctaatcgc
    10861 tcggttaaga agaaaaacga aactaagaag gaggtaaaag atgaaaccaa aaacaagcgt
    10921 tctcagagga aataaggcca atagaaaatg gcatttaatt gatctcgacg gcaaaatatt
    10981 aggacgagta agtaccgaga ttgcgagtct gctaatgggt aaaaataaac caactttttc
    11041 ttatcatcga gacgatggcg attatgttgt ttgtgttaat gccgcgaaaa ttcaagtaac
    11101 aggcaaaaaa cttcggcaga aaagccattt ttggcatact ccctggccag gccatttaaa
    11161 atcactgact tgggaagcat tattaaaaaa agacccgcgg caactgattt atctaagtgt
    11221 ggctaatatg ttgcctaaaa atagattaag agatagccgt ttaaaaagat tgaaagtttt
    11281 tacaggttta aatcaccctt atggcgacaa atttaaaaaa gacaactaaa acttatactt
    11341 atgcgattgg tcggagaaaa tgtgccgtgg cggcaataaa actttttaaa ggaaaaggag
    11401 aaatgacagt taataagtca gccatagata aatattttcc attatcgtcg gataaaatta
    11461 tttatgagcg gccgttagta gtgactaata ctttaggcaa atattattat gaggccagag
    11521 ctaacggtgg cggtaaacat ggtcaattag aagcggttac tttggcagtc gcccgagccc
    11581 ttaaaaaggt aaatgacaag tttggtccta ttcttcggac caatggctta ttgacagttg
    11641 attcgcggat aagagagcgg cggatggtcg gtaccggcgg taaatctcgc cgaatgaaac
    11701 agtcaccaag gcgataaatg aaatctacct gccgtgatat actcttttat gtcttatttg
    11761 gttttggcta ttgttcaagg aattactgaa tttttgccga tttcctccac cggtcatctc
    11821 aatctttatc aacacttttt tcatttaacg ccgtctctca gtcttgatat ttttcttcat
    11881 ggaggaactt taatagctgt cctttttgtt tttagaaaac aatttaggta tttctggaaa
    11941 aatttttggt atattattat ggcttcaatt ccggctggaa ttatcggtat tttatttaaa
    12001 gaacagttcg atactctttt tgccaatgtt aaacttttgc cgtttttttt ccttatcact
    12061 actatgtttc ttttcttaac caaaaattta aagactaatc agagccgatt gacttataaa
    12121 aatgccctta ttattggtat atttcaggca atagctcttt taccgggtgt ttcccgttct
    12181 ggagcgacaa ttgccgccgg ccttttagtt ggtttaccgc cgttagaggc ttttaatttt
    12241 tcctttgctt tgtttattcc cgcgactttg ggagcaattg ttttggctta taaagatata
    12301 gtctttttcc cttccttgat tataccgaca attttgacca taattaccgg tattttagct
    12361 ctcaattttc ttaaaaagac tttaatttct aaaaattttt ggaaatttag cctttacact
    12421 tttattttag ccctaatcct tcttctagtt ttaaattttt aattatcgtt agatttctcc
    12481 gcttaaatat tccctccaaa tctggtatag ctctgctgtc gcaacagcat ctctggtcac
    12541 ataatcagcg atttgttgaa atttcttttg ataaaataaa ttgctgacct ctgcaccagt
    12601 aactccctta tccttaggat cgtcgatgcc aaaaacccgg caaagcatct ccaacttaaa
    12661 gggctttccg ttttgtttta acctatcttc caggtcaata aaattttgac tgtatttttt
    12721 aatttcaaaa ggaattttga ctcggttaat ccctgatcgg ataattaaat atggaatatc
    12781 gaaattattg ccattaaagg taacaaaacg ttggactttg ctggctaatt cccagaattt
    12841 ttccaataat tctttttcat ttttaaccac aatgtaggta aaattatttt tttctggaca
    12901 gagtttcttt tccccaatta cgatagtaat tccctgtttt ttatcgggat tataacaacc
    12961 ggcagcgcat attttcccaa aaatcgcata caaagctgtt cgtttttttg atttttcctt
    13021 atcctcctcg tatttttcca gagtattaag caaataatct tgttgctgct gatctagttc
    13081 ttcgaattta tctcctactg tttctaaatc gaaaaccatt gttggtatta cggttttaaa
    13141 attagctatg tcctcaccgt taacaatttg acaggcatta ataatcaagc ttagtttgcc
    13201 gttgtattca tctgtttgtc ctgacacttc gataactttg ccaatttcaa aattacaagc
    13261 cggcagagca ttatcccata ttcttccgat aataacacca gtactatcat ttagttccaa
    13321 atcataataa ttattaccgt tcctagcagt ttttttgtca acttttttga ctaaaaaatg
    13381 ttcttcatta attttttgat tggctgaaag ttgactgatc ataagcaatt atactcttcc
    13441 tcatctgcct attattggct gataattgat taactataat tatacaataa ataaaggcaa
    13501 aatttggtaa aataccctag acaagataat tttttaatta gaaataaatt aaaaatttta
    13561 aatttattaa cggggattgg cgcaattggc tagcgcgctt gcatggggtg caagaggttg
    13621 ctggttcgaa tccagtatcc ccgactttgg ctcactcaag gcgattctgg ttttcgctat
    13681 caccgaattt tatgctttgc tttggcaaaa atcaccaagg ctttgggtgt ttcaattgaa
    13741 gagttgataa aatagtatgg atcaaaagca gattatcaaa caaattagga aagagcttaa
    13801 aaataaagcc agtacaaaaa ctgataaaga aagagagaaa gcgaaagcgc tgtttggtaa
    13861 tgaaactaaa cttttagctc ttagagttcc tgaaacaaga aaaatagcag aatcttttgg
    13921 caaagaactt aaaaaagaaa aggattttga taccgcctta aaagtagcag accagcttta
    13981 taaaagtggc gtattcgaag aagcaacggt agctgaatca attttaaagg aatttaaaaa
    14041 ctactttgat aatttaactt ttgataagtt tgatgaatgg atagattaca taaagaattg
    14101 ggcgaatatt gatatgcttt gtggctggct gattacgcct ataattttga aggacgacac
    14161 caagcttaaa acaatactgc gatggacaaa atcaaaaaac cgttggcgca gacgagcggc
    14221 gatcgtatct tttgtaaagc cggtgaagaa aggaaagtat ttgaaacaag cattagaggt
    14281 tgccgaggaa cttatacaag acgaagacga gatggttcaa aagggggttg gttggctttt
    14341 gaaagaagcg ggtggagttt ttccagatga gacaaccaga tttcttatga gatggcggcc
    14401 aaaaatttcc aagctcgtgc tccgtcttgc ccgcgaaaaa ttacctttag ataaaatttt
    14461 ttatattaaa aaggtcgaat aaattttaat taaagaaaaa gagggtgagg tgaaaaaaaa
    14521 tatgaacgtg gcacagccgt aaggacagtt ttactaaagt tgcgctatgc cagcgccaaa
    14581 acctgagaat tttggcacta atgctgatgg aagcaaaagc gaggaatatt gtcagtattg
    14641 cttccaaagt ggcaaattta ccgagccaaa tatcactatg ggcagataaa aatgcccgaa
    14701 gcgcaaattg agcagacaaa aatttattcc agtgctcaaa agatagaggg ttaaataaat
    14761 ttatgtccaa aattcaagaa atcttaaaat taaatcttaa gccaaaggaa aaaatttcga
    14821 ctttggcgga ggagataaag aaagataaaa agtttatcgg cagacttatt gcctattttg
    14881 aatctgcctc tgccagtgag caaggacatt tgattgaatc tttagagtat gcctcgcagg
    14941 ataagccgga atttgttgcg ccttatttta attttgtgct tgagcatttg gctgacaaag
    15001 caccacgggt gaaatgggag gcggcaagaa taatcgctaa tctggcagaa aagtatgcgg
    15061 acaaaactga aaaggcgatt cctgaacttt tgaagaacag cagcgataaa ggcacggttg
    15121 ttcgctggag cgcggctttt gctttgggtg agattgctaa aagtaattca aacgcgcaaa
    15181 aaacattgct tcctaaaatg aaagaacttg taatgaaaga aacgaataat ggcgtaaaaa
    15241 atgtttatct taaggcgtta aaagtaattg aaaaagaaaa gtagctatga acaaaataat
    15301 tttaggcaca atccacggat tagtatttgg gatgattgat gtttgaaata cgaaaacaat
    15361 cgaaagagga ttgaggctga atttggctgc taattggcgg aaattagcta acgactggtc
    15421 cagccgccgt caactactaa aaccgcccca ttgacaaaat cggcttcatc agaggccaaa
    15481 tagacggccg cagcggcaat atctgccggt tcacctaggc ggggataagg agttgaactt
    15541 tctacaaatt gtttactgtt tggatcagtt gtcatttcct tagtcattgc tgttttgata
    15601 acccccggac agatacaatt aacattgatt ttttggggtg cataatctaa tgccatctct
    15661 ctagtcagag caataatccc gcccttagtc gcacagtaag cgccgctttg agcaaaaccg
    15721 actaaaccag cgattgaagc tgtgttaata atttttccct gaccctgttt aagcatttga
    15781 ggtaaaacag cccggctgcc cagaaaaaca ctttttaaat tgacattaat tgtttgatcc
    15841 cattccgctt cggtcatttc gtggagatta aaggtcctat aaataccagc attattaaca
    15901 agaatatcaa cccggctgaa ttgatctaaa caggttttaa ccatctgctc aatttcagtc
    15961 gcttggctaa cgtcagtttt aacaaacaga gcttcacccc ctgcttgttt aatttgagcc
    16021 tcagtggctt gtccaccttc taccgataaa tcggcaataa ttaatttggc tccctctcgg
    16081 gcaaaagcta gagctatctc ccggccgata cctgagccag ccccggtaac aatggcaatt
    16141 ttgttttcta gacgcatatg aaaaagaata tcactgataa tacctctttg tcaatggatt
    16201 atttatttta cattgacaag aacaataaat cctgctatca ataagaagtg atgaagaaaa
    16261 aagctaagaa ggagaaagtt actgcctcag gtgatttggc agataagaag ctagaggcgg
    16321 ttaagctggc catggagcag attaccaaag cttatggtgc cggcgcgatt atgagaatgg
    16381 gtgaacaggc tggtttaggt caagtggaag ttattcctac cggttgttta cccttggatg
    16441 ttgctttagg tgttggcggt ttacctcgtg gccgggtaac agaaattttt ggtccagaag
    16501 cttcagggaa aacaacttta gctcttcgag tggtagctga agcccaaaaa atgggtggta
    16561 cggccgcttt tattgatgcc gaacatgctc ttgatccggc tcgagcggcc gttattggag
    16621 tagatttaaa tacccttttg atttctcaac ccgataatgg ggaacaggcg ctggaaatcg
    16681 ttgaaacgtt ggtccgttca aacgccgtag atgtgattgt tgtcgattcg gtggcggctt
    16741 tagtacccaa agcggagatt gaaggagaaa tgggggacgc gatgatgggg gttcaggccc
    16801 ggctaatgtc gcaagctttg cggaagttga cgggagctat ttctaaatcc cgtgctgtag
    16861 tgatttttac taaccaaatt cgccagaaaa taggggttat attcggtaac cccgaaacaa
    16921 ctcccggcgg tctggccttg aaattttatt cttcggtccg gattgatgtt agaaaaatcg
    16981 ccaatatcaa aacggctgcc ggcgagccag tcggttccca gcatcgggcc cgaattgtca
    17041 aaaataaagt tgccccgcct tttaaagaat gtgaatttga tattaccaat aatgaaggtt
    17101 tgtccattta tggtagtttg gttgatttag ccgttgagca gggtttaatt gaaaagtcag
    17161 gtgccttttt taaatttaat agtcaaagta ttcaaggacg agagggaatg aaaaaatacc
    17221 ttcaggatca ccctgaagct gtggctaaat tacgggacga tatttggagc aaagtcaaag
    17281 ccgataatga aattgcaaag tctcaaaccg tcaagaatta aaaatcgggt caatctggtt
    17341 tttgatgatg gttcctatct tcccttttta gttgatgaag ctctgaaact aggattgaaa
    17401 accggccaag aagtgaattt tatggaactt aaagaaaaat ccaccaatta tttggctcgg
    17461 gagtatgctc tccgccaaat tgctttgtca cccaagacag aaaaactttt acgacgaaaa
    17521 ttattacata aatttccaaa ttttttgcca gaccaactta ttcaggaatt aaaaccgtat
    17581 ttagaagaga cccaatatat taattatatc ttgaaaagat ttaaagatcg gtctaatcgg
    17641 gaaattagtt atcgattaaa attagccgga attaactatc aatcccaaga aaatgactta
    17701 gctaaaatca aagccttatt aaaaaagaaa cccaagacta cttttgccag tttaattcgt
    17761 aagggttttg cctacgatga cgttaaatcc acatttgata aattaacctc aatcgagtaa
    17821 aatttactgt ctactattta ctatttacta tttactattt actttaatgt ccaccttgtt
    17881 tattgacttt ttgcttatgc tagtctgatt tttggtgtct taaagtaaga aggagcgttt
    17941 atgtttgatt cattcattaa aacctttaag aatttattaa cgccgactgg tttagaaact
    18001 cctaaagaaa gtaataaagt tgaatatact aaagttaaac cagaagttaa aactgaagta
    18061 gtttcggcaa aagaaaattt agccgaggcg gaattgttgc gacgtttagc cttgctggat
    18121 gaaaaagaaa aatatttgat tagaaaggaa aaaatgttag atagtcggaa tgctcaaatt
    18181 caggctaagt tagcccagat cgatgacgtt tatcggaaac agttggaaaa attagaacag
    18241 atttccggtt tggatcagga aaaagctaaa caattaatta tttcctcaac agaaaaaaga
    18301 atggccaatt gggtagcaaa gaaaattgag gaagccaagg aattaattaa acagagagag
    18361 gatgaggaag ctaaggaaat tttaacggaa gccttaaagc atggagtgac cgatactgtg
    18421 gctgaatata ctgtttcgac aatcaccctg gccgatgaaa aaattaaagg caaggtaatt
    18481 ggccgcgaag gccgtaatat ccatgccttt gaaaaagcta ccggggttga actagagctg
    18541 gatgaaggga atgatattcg tatttcctct tttgattcca ctcgaagaga aattgctcgg
    18601 ttggctttag aaaaactaat tcgtgatggt cggattcagc cgttgcggat tgagcaagta
    18661 gttaatcaaa ccaaacagga aatggacaag attcttttgg ctgaggggaa aaggatttgt
    18721 caagaggttg gtgtttttaa tctgccgatt gatttaatga aagaaatcgg taaatataaa
    18781 tttcgctttt cttatggtca aaatttagct aaacatagtg ttgaggcgac taaaattgcc
    18841 gtggatttgg cttacgaatt aaaggctaat gtgggagtgg ttcgtttagg ggctctactc
    18901 catgatatcg gtaaggttat ttctgatcaa gagggaactc atgtggaatt aggtgttaat
    18961 ctattacacc aatataaatt accggaagcg gtgattaatt gtattgccga acatcatgaa
    19021 ggtaaggaat ttagttctat cgaatctgtt attgtttatt taggtgatgc cgcatctggg
    19081 gttagacccg gggcccgtta cgaggttcat gaagagtatc ttaaacggat gcagaatatt
    19141 gaggaaattg ccaaacagtt taatggggtt cagtcagtag ctgcttatca agccggtcga
    19201 gaggtaatgg ttattgttga tcccggaatg gtttctgatg ctgaggcaga agttttatcg
    19261 caaaatattg ctgaaagatt agatgaagaa gccaagtggg cgggtcaaat tagagtaaca
    19321 gttattaggg aacttaaggc tagttctata gtgtcaggaa ataaaatcag taaaaatgcg
    19381 gggaaaaatg aatgatgact cttgcgtttt aaattaatta ctctaaaatt agggtgaatt
    19441 attaattaag agaaaaaatg actaaaaaag atctaattga agtcgtagct caaaaagcta
    19501 aactgtccaa aaaagctgcc aagcaagcgg ttgagacttt cctgaaagaa atcatgaaag
    19561 ctctttccgc cggcgaagtt gttaaacttt ccggttttgg aaccttcaaa accaaaatgt
    19621 tcaaggccaa gacaattaag gccattggtt ccaaagaagc taaaaagatt gttgcccgcc
    19681 gtatgcctcg ttatatcccc ggaaccaaaa tcaagaaaat ggtgcgttaa ggactgtttt
    19741 tgaattttaa aaattccccg ccatttggtg gggaatttt
//
LOCUS       DAOOIE010000002        11347 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_5214_length_11347_cov_4.147007, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000002 DAOOIE010000000
VERSION     DAOOIE010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 11347)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11347)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11347
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5214_length_11347_cov_4.147007"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PK131_00195"
     CDS             <1..445
                     /locus_tag="PK131_00195"
                     /inference="COORDINATES: protein motif:HMM:NF013527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HPD44591.1"
                     /translation="ASRGLLEAKIIGPAEVKEKLGVAPVQVIDLKALMGDLSDNYPGV
                     PGIGPKAAVNLLKQFNSLDEVYSNIDKIQPSLAGRLISYKDSAYLSKKLATLVNNIPL
                     NFDLNTAEFTNDTIAGLLDLFKQYNFKSLLNRLKKNKKVEKLSLF"
     gene            455..1585
                     /locus_tag="PK131_00200"
     CDS             455..1585
                     /locus_tag="PK131_00200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD44592.1"
                     /translation="MRVALVHDYLKEYGGAESVLEVLSEIFPQAPIYTTVYLPEFFGP
                     HRSRLETKWRGRIRTSFFQNFPLVAKLISPLRFFSPLTFRSFDFSGFDLIITSATGAY
                     FPNSLNKKSAKLFCYCHTPPRYLYGLPTARNFSKNPLLRPIIDLVNCFWRRLDKKYAT
                     NVDQYIANSATTAARIKKFYNRDAVVINPPVNLPQLPNSRSTIHNLRSNFYLTGGRLA
                     RAKRYDVAIQACNQLGQPLKVFGRDFAGYLAELKSIAGKTIEFIGEIDDQTKFKLYSQ
                     AKAFIFCSDNEDFGIMPVEANACGCPVIAYRSGGVAETIIDGKTGIFFNDLTVDSLVE
                     AIKKFEKMKFNSRDCLTQAQRFSKKIFIKNLQSLISDPPLHF"
     gene            complement(1547..2512)
                     /gene="trxB"
                     /locus_tag="PK131_00205"
     CDS             complement(1547..2512)
                     /gene="trxB"
                     /locus_tag="PK131_00205"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006805733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPD44593.1"
                     /translation="MGEIYDTLIVGSGPAGLTAAIYNVRAGLKTIVITGNQPGGQLTL
                     TTMVENYPGFPHGIGGIKLMMDMQSQLKNLGGQIKTGVVKTINPWSDKNHYFLNELEN
                     GEEIQSKAVILATGSILKWLGLPKEKELIGRGISGCATCDGAFFKNKTVAVVGGGDTA
                     CGEANFLSRLASKVYLIHRRDQLRASLAEQKRVLKNSKIEIIWNTEVIELNPNIQASS
                     TQLQTIKLKNNKTGGEKILKLDGLFVAIGYQPATDFVKDLVATDDNGHIIICQNSQYK
                     MMTSMPGIFAAGDCDNINYQQAVTAAGEGCQAGLEVERWIRDQRL"
     gene            2613..3482
                     /gene="mutM"
                     /locus_tag="PK131_00210"
     CDS             2613..3482
                     /gene="mutM"
                     /locus_tag="PK131_00210"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HPD44594.1"
                     /translation="MPELPEVETIRRQLKPKIVGKTIIGVKIFCNKSFQGDPKKIIGR
                     KVLALNRVGKQLVIDLSGNLVILVHFKMTGGFFFQPNKYTRVALYLSSPFIKQRKSTR
                     HPAGSSSPDKKEGFEGFFFSDPRKFGWLKVLTKRSWLSYQKNLGMDILSFSFTPEYFY
                     QQLQTSSRPIKQVLLDQTKMAGAGNIYANEALFLAKIHPLTSSRDIPRDQAYSLNHYL
                     KWVIMDSISHNGSTARDRGYLQPNGQSGSHQNYFHVYQKEGEKCSTCSAKIKRLKIGG
                     RSTFYCSKCQKIK"
     gene            3464..4075
                     /locus_tag="PK131_00215"
     CDS             3464..4075
                     /locus_tag="PK131_00215"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPD44595.1"
                     /translation="MSKDKIKTIVFDICDVVWDLTPSRNEFYTEVSQIFHQDLFSVME
                     DFLKVYQQLQLNQMTLRDWFEQKGVNYSQKQINNLIEAIYQDDKFMGYFNQEVIDLIS
                     ELRRFCQVGCLSNIENFLGKYFEKHFNPLFDYTIFSYNIGHRKPEIEAYQEIFKKINC
                     SPEEVVFIDDKERNLIPARQTGFNCLLYTNSAQLKTDLEEFLL"
     gene            4083..4697
                     /locus_tag="PK131_00220"
     CDS             4083..4697
                     /locus_tag="PK131_00220"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPD44596.1"
                     /translation="MPEIEGFIFDVGGVLFKTDRFWRDVFPEIGRRVKELPSQLIQRY
                     RQKETNFETIPSNGCNFSRLAAVLDLNISSQYLEEMINKFLGKEKLFRYLDANVIALV
                     GEVRQRGYQAFCLSNVANFFDNYLKNNINPLFDQSFYSYEIGLRKPCPEIYEAVLKKV
                     EISPQRLVFIDDRQKNLDYPYRIGINCLLYTNPTQLKTDLNKFL"
     gene            complement(4754..5314)
                     /locus_tag="PK131_00225"
     CDS             complement(4754..5314)
                     /locus_tag="PK131_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPD44597.1"
                     /translation="MSQTQQNLLKAFAGESQARNKYTQFAKIARKQDQEFIARVFEET
                     AENERVHAGELFELITEPVMTAGDLTITPFSHSTVENLREAAKGENYEWTEMYPNFET
                     IAQTERESEPARLFGKLKEVEKLHEERYIILATKLDNDTLYNSETELEWKCLNCGYIH
                     KGKTPPTKCPLCQKPFSWYMPIGLIK"
     gene            complement(5337..5765)
                     /locus_tag="PK131_00230"
     CDS             complement(5337..5765)
                     /locus_tag="PK131_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44598.1"
                     /translation="MKKENKNKHLIIIVGLVFIIAIISGGIGYKIGEKRSIARRTFQF
                     RGRENQPMTRDRTKIINGEVVSIDDKSITIKMADGSSKIILLTDKTIVNKAEITNIAD
                     LTIGEKVMIFGTDNSDGSISATNIQLNPQNASAKPNPFEK"
     gene            5853..7520
                     /locus_tag="PK131_00235"
     CDS             5853..7520
                     /locus_tag="PK131_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44599.1"
                     /translation="MADFSPDVLYSNDNSPLSPPIQDLIRQYRESFLTKPRGEIATIH
                     VDEIASKVAFLYERIRKIIDWKEDNLLRRSAIERILKRNLIGEVSKFKPIFKADEATL
                     GERLVLELVRGGHLPNNQIPEEKINEVQTALKKYIYFLKNIPLVTSFVNKKKINLFDW
                     ILEVAACEIEEIVSPATKENLLIETMTALMNERIRVMPELALSDEEKLTQTYIAVHRT
                     LFDLDDSLITYRLLKRRYPQWTNPSETFISETAVNIFSLWESLEKELNHPLSKDFFDI
                     CDKTDTIFILLGDILDHFKDEPDQLEAALASEERLKALLSRFYQKRRQTLKSRLFKIA
                     IFSSLSVLISNCFSYFIVEVPLALLLYDRFSLIATIVDFILPATAMFLLVAIIKPPSA
                     TNFKKVVELTFNFVYSSREKNIYEIKPKKKTSLWTTFWIALFYLIGGTVFFGLVAWGF
                     YKAKIPVISVILDTVVIAINVFAALLVRNKAKEITVEEKTTFWEFILDTISIPVAQVG
                     SWLARKWKEYNVAAAFFNAAIEMPVITIIGFIEDWRTFLREKKGSLH"
     gene            complement(7517..7891)
                     /locus_tag="PK131_00240"
     CDS             complement(7517..7891)
                     /locus_tag="PK131_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005363647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HPD44600.1"
                     /translation="MYSKKVMQYFRKPKNFGQIKDADGIGQVGNIVCGDVMKLYLKIK
                     NKTITKATFETFGCVSAIASSSILTTMIKGKTLEEASKITKEEVVQALDGLPALKIHC
                     SVLAVDALKAAIEDYQKTTHKT"
     gene            complement(7893..9059)
                     /locus_tag="PK131_00245"
     CDS             complement(7893..9059)
                     /locus_tag="PK131_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPD44601.1"
                     /translation="MKKPKIYLDYAATTPVDPRVIKTMLPYFLAKIGNPASMHRMGRD
                     NLRVLDKCRRTAAGFIGAQSATEIIFTGSATESNNLALKGTMWANRSRGNHLIVSQIE
                     HDCVLESAKWLAQNGFEVTFVPVDQYGLVHPEEVEKAITSQTVLVSVMQANNEIGTIN
                     PVTKIAQICHQHGVYFHTDAAQTCGKIPVDVQKIGADLLTASSHKMYGPKGAALLYIK
                     KGTKIEPWMHGGGQEEGMRSSTVNLPAIVGFTQAMTIAQKTMTTESVKLTKLRDKLVE
                     HILKAIPTASLNGHPTQRLPNIVSFRFAYIEGESLVYLLDEDGIMASTGSACSSPKLT
                     PSHVLIACGLKKEEIHGSLRISLGRWTKEKDIDYLIQKLPLAVQKLNKLSPFLN"
     gene            9082..9426
                     /locus_tag="PK131_00250"
     CDS             9082..9426
                     /locus_tag="PK131_00250"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HPD44602.1"
                     /translation="MKCTCNCQVKCRLLGSLEQEIMEILWASPQPLKPAVVQQTLKNK
                     YAYTTVMTVLNRLNNKKIVNRRVKGKAYYYSPCLKKEEFVRSCLQDLFARLKLSYGSV
                     FIKEAVDYLLRL"
     gene            complement(9455..10243)
                     /locus_tag="PK131_00255"
     CDS             complement(9455..10243)
                     /locus_tag="PK131_00255"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPD44603.1"
                     /translation="MKTELSIARFYRKDECLNSSVPAPIIHWFETVTKDNWLKTNNKE
                     RRAKIEYKINHTYKTVEAGWEIMAKESSVDWDKSLGTIICLLHDIGRFPQARQSNTFI
                     DAQSGIDHATLGAEMFLKQNFDIPHKEEIVEAIFWHSRKEYPGRSNYVRLVKDADKTA
                     LFRDFDFMEKNDVPAFHLSGNQLSPKRLASFLNGEFLPDRSFNSLADWYLFNAGWFWD
                     LNFATTKQIWRQEKFAPMLLAKLKSIGVDPDQLQLIESKLTSFL"
     gene            complement(10240..11019)
                     /locus_tag="PK131_00260"
     CDS             complement(10240..11019)
                     /locus_tag="PK131_00260"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HPD44604.1"
                     /translation="MTKIFADTADLSLIKELLAQGVPIDGVTTNPSLLQKAMGEKKFS
                     PQELDRIYINLLREIRKILPQGSISGEIFITPKTPKEEIYRLAEKMSDQVSRIHIKIP
                     IFKAGLAAAADFVKRGGRVNMTLCFSLEQAAAVERIVGYLSKNDQVYVSPFIGRLNDI
                     GLRGMSLIENIQRQFTKDKAKTKLLAASIRSKDQFYDCLALKVDIVTVPPQILFEWKG
                     DNFKRPKNIKYVAPHRHLNIKHPLTDKGFDLFIQSQQALLK"
     gene            complement(11019..>11347)
                     /locus_tag="PK131_00265"
     CDS             complement(11019..>11347)
                     /locus_tag="PK131_00265"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPD44605.1"
                     /translation="MYLNNLKDQFGDNLVIPDNFRYEWSYVTHLFETPFYCYAYNFGQ
                     LLSLSLYSEYKKDKNYAKVIEAILAAGGSQDPVKLLLDLGINIKQENFWQNSFDFIKS
                     LIREIN"
BASE COUNT         3285 a         2120 c         2134 g         3808 t
ORIGIN      
        1 cgcctcgagg ggtctattag aggcgaaaat tattggccct gccgaggtga aggaaaaatt
       61 gggagtggcc ccggttcaag taatcgattt aaaagccttg atgggtgatc tatcagacaa
      121 ttacccaggt gttcccggta ttggtcctaa agcagctgtt aatctcttaa aacaattcaa
      181 tagtcttgat gaggtttata gtaatattga taaaattcaa ccttctcttg ctggaagatt
      241 aataagttat aaagattcgg cctatttgtc aaaaaaatta gctacattag tcaataatat
      301 tcccctcaat tttgatctta atactgctga atttactaat gatacaatag cgggactgtt
      361 agatttattt aagcaataca attttaagtc tttattaaac cgtttaaaaa agaataaaaa
      421 agtcgaaaaa ttatctttat tttgaaattt atttatgcga gtagctctgg ttcatgatta
      481 tttaaaggaa tacggtggag ctgagagtgt tctggaggtt ttatcggaaa tttttcctca
      541 ggctcctatc tataccaccg tttacttgcc tgaatttttt ggacctcacc gctcccgttt
      601 ggagaccaag tggcggggac ggattcgaac cagttttttt caaaattttc ccttagttgc
      661 taaattaatt agcccccttc gtttttttag tcccctgact tttcgctctt ttgatttcag
      721 tggttttgat ttaattatta cttcagccac cggggcttat tttcctaatt cattaaataa
      781 aaaatcggcc aagttatttt gttattgcca tactcctcca cgttatctct atggattgcc
      841 gaccgcccgt aattttagta aaaacccatt actacggccg attattgatt tggtaaattg
      901 cttctggcgc cgcctcgata aaaagtatgc tactaacgtt gaccaatata ttgctaattc
      961 agctaccact gccgccagaa ttaagaagtt ttataaccgc gacgctgttg ttattaatcc
     1021 cccggttaat ttaccccaac ttccaaactc acgatctacg atccacaatc tacgatctaa
     1081 tttttatctc accggcggcc gcctagctcg agctaaacgt tatgatgtgg ctattcaggc
     1141 ttgtaatcaa cttggccagc cattaaaagt ttttggtcgt gattttgctg gttatttggc
     1201 agaattaaaa agtatagctg gtaaaacaat tgaatttatt ggtgagattg atgatcaaac
     1261 taaattcaaa ttatattccc aagccaaagc ttttattttt tgttctgaca atgaagattt
     1321 tgggataatg ccggttgaag ccaatgcctg tggctgtcca gtgattgcct atagaagcgg
     1381 cggagtagca gaaactatta ttgacggcaa aacaggtatt ttttttaatg atcttactgt
     1441 tgattcttta gttgaagcta tcaaaaaatt tgaaaaaatg aaatttaatt ctcgagattg
     1501 tctcactcaa gcccaacgtt tttctaaaaa gatttttatc aaaaatctac aatctttgat
     1561 ctctgatcca cctctccact tctaatccag cctgacagcc ctcgccggca gccgtaacag
     1621 cttgttggta gttaatattg tcgcaatcac cagcggcaaa gattcccggc attgaagtca
     1681 tcattttgta ttgcgagttc tggcatataa taatatgccc attatcatca gtagcgacta
     1741 gatctttaac gaaatcagtt gcaggttggt agccaatggc gacaaataag ccatctaatt
     1801 ttaaaatttt ttccccacca gttttgttat tttttagttt tatagtttgt aattgagttg
     1861 acgaagcctg aatattgggg tttaattcaa taacttctgt attccaaatt atttcaattt
     1921 tagaattttt aaggaccctt ttttgctcag ctaaactggc tcttaattga tcccggcggt
     1981 gaattagata aactttgctg gctaatcttg ataaaaaatt agcttcaccg caagcagtat
     2041 caccaccgcc aactacagcc acagttttat ttttaaaaaa ggcgccatca caagtagcac
     2101 agccggagat accccgacca atcaattctt tttcttttgg taaaccaagc cattttaaaa
     2161 ttgatccagt tgccaaaata accgccttag attgaatttc ctccccgttt tctagttcat
     2221 taagaaaata atgattttta tcagaccagg ggttaattgt ttttactacc ccagttttaa
     2281 tttgaccacc caaatttttt aattggcttt gcatgtccat catcaattta ataccaccaa
     2341 taccatgagg gaaccctgga tagttttcta ccatggtagt taaggtgagt tgtcctcccg
     2401 gctgattacc ggtaataaca atcgttttta aaccagcgcg aacgttatag attgcggctg
     2461 tcaatccagc tggcccagaa ccaacaatta aggtatcata aatttcgccc atatttttac
     2521 aatcgattga ttactaattt ataattcact tatgaatttc attttaacat ttgttcccct
     2581 ccttattaaa gagaagggtt gaggagagat tgatgcctga gttacctgaa gtggagacta
     2641 ttcgtcgtca acttaaacct aaaattgttg gtaaaactat tattggagtg aaaattttct
     2701 gtaataagtc ttttcagggc gatcctaaga aaattattgg cagaaaagtc ctggctctta
     2761 accgggtagg taaacaatta gtaattgatt tatccggcaa tttagttatt ctagttcatt
     2821 ttaaaatgac aggtggtttc ttttttcaac ctaataaata cacccgagtg gccctgtatt
     2881 taagttctcc ctttattaaa caaaggaaat ctacccgcca tccagcagga agctcgtcgc
     2941 ctgacaaaaa agagggtttt gaaggatttt ttttctccga cccgcgcaaa tttggctggc
     3001 tgaaagtttt aaccaagaga tcttggttgt cttatcagaa aaatttagga atggatatcc
     3061 tttcatttag ttttactcca gaatattttt atcaacagtt acaaaccagt tcccgaccga
     3121 ttaaacaagt tttacttgat caaacaaaga tggccggcgc tggcaatatt tatgccaatg
     3181 aggccctgtt tctagccaaa attcatcctc taacttcttc tagggatatt ccccgtgatc
     3241 aggcctatag tttgaatcac tatctaaaat gggtaataat ggattcaatt tcacataacg
     3301 gctcgactgc ccgcgaccga ggttatcttc aacctaatgg ccaaagtggc tcccaccaaa
     3361 attattttca tgtttatcag aaggaaggtg agaagtgttc gacttgttct gctaaaatta
     3421 agagacttaa aattggcggc cggtcgactt tttattgctc taaatgtcaa aagataaaat
     3481 aaaaactatt gtttttgata tttgtgatgt ggtttgggat ttgactccgt cgcgtaatga
     3541 attttatacg gaagttagcc aaatttttca tcaagatttg ttttcagtta tggaagattt
     3601 tttaaaagtt tatcaacagc tgcagcttaa tcaaatgacg cttagagact ggtttgaaca
     3661 gaagggggtt aattactccc aaaaacaaat taataattta attgaagcta tttatcaaga
     3721 tgacaaattt atgggttatt ttaaccagga agttattgat ttgatatcag agttgcgaag
     3781 attttgtcaa gttggttgcc tttcaaatat agaaaatttt cttggaaaat attttgaaaa
     3841 acattttaat cctctttttg attacactat tttttcttat aatataggtc atcgtaaacc
     3901 tgaaatagaa gcttatcagg agatttttaa aaaaattaat tgctctcccg aggaggtagt
     3961 tttcattgat gataaggagc gtaatcttat ccccgcccgc caaacaggtt tcaactgtct
     4021 gctttatact aattctgccc agcttaaaac tgatctcgaa gagtttttgc tataattcct
     4081 ccatgcctga aattgaaggg tttatttttg atgtaggcgg tgttttgttt aaaacagata
     4141 gattttggcg ggatgttttt cctgaaatag gtcggcgggt aaaagaattg ccttcacagc
     4201 ttatccagcg ttatcgtcaa aaagaaacta attttgaaac tattcccagt aatggttgta
     4261 attttagccg attagcagcc gttcttgatt taaatatctc ttctcaatat cttgaagaga
     4321 tgattaataa gtttttgggg aaagaaaagt tatttcggta tttggatgcc aatgttatcg
     4381 ctctggtcgg cgaggtgaga caacgcggct atcaggcctt ttgtctttct aatgttgcta
     4441 atttttttga taattatctc aaaaataaca ttaatccctt gtttgaccag tctttttatt
     4501 cctacgaaat aggtctccgc aaaccttgtc cagaaattta tgaagccgtt cttaagaaag
     4561 ttgaaatttc ccctcaacgg ctggttttca ttgacgaccg gcagaaaaat cttgattatc
     4621 cttatcgcat cggtatcaat tgtctgcttt acaccaatcc gacccagctc aaaactgatc
     4681 tcaataaatt tctataactt tttgtcgctc ttcctttttc aatgggatct gcctgccggc
     4741 aggagaaagg tttttattta attaatccta tcggcatata ccaactgaac ggtttttggc
     4801 acaatgggca tttggttggc ggggttttgc ctttatgaat ataaccacag ttgagacatt
     4861 tccattccaa ttccgtttct gaattgtaaa gagtgtcgtt gtctagttta gtcgccaaaa
     4921 ttatatatct ttcctcgtgc agtttttcta cctcttttaa tttgccgaac aatctggctg
     4981 gttctgactc tctttctgtt tgagctattg tttcaaaatt agggtacatt tctgtccatt
     5041 catagttttc ccccttagcc gcttctcgta gattttcgac tgtactgtgg gaaaaaggag
     5101 taatggttaa atcaccggcg gtcattactg gttcggtaat taattcgaat agttcaccag
     5161 catgaacccg ctcattttca gctgtttctt caaaaacccg ggcgataaat tcctgatctt
     5221 gttttcgggc aattttcgca aattgagtgt atttatttct tgcctgtgac tcaccggcaa
     5281 aagctttcag aagattttgt tgcgtttgac tcataacttc agtatacctt attcacttat
     5341 ttttcaaaag gatttggttt agcagaagca ttttgcggat ttaattggat attagtggcg
     5401 ctaatactgc catcactatt atctgtgccg aagatcatta ctttttctcc tattgttaaa
     5461 tcagctatat ttgtaatttc tgccttattt acaattgttt tatcggttaa taatatgatt
     5521 ttacttgaac catcggccat tttaatagta atacttttat catcaatact aactacttca
     5581 ccattaataa ttttggtccg atctctggtc attggctggt tttctcgtcc cctaaactga
     5641 aaggttcttc tagctatact tcttttttct ccaattttat atcctatacc accgctaata
     5701 atggcaataa tgaaaactaa accaacaatg atgattaagt gtttattttt attttctttt
     5761 ttcataactc tgaatataat ccttcatcat gaattatatc tgaaagtttt ctttccatta
     5821 gttaattccg ttaattttgt ttataattaa acatggccga ttttagtccg gacgttttgt
     5881 atagtaatga caattcgccc ctttcaccgc cgattcaaga tctaattagg cagtatcgag
     5941 agagtttcct aaccaaacct agaggggaaa tagccaccat ccatgttgac gaaatcgcct
     6001 ctaaggttgc ctttctttat gaaagaataa gaaaaattat tgattggaag gaagacaatt
     6061 tactaagacg gtcggcaatt gaaagaattc ttaaacggaa tttaatcggt gaagtgtcta
     6121 aatttaaacc gattttcaaa gccgatgaag cgactttggg ggaaagattg gttttagaac
     6181 tggtgcgggg cggtcatctg cctaataacc agattcctga agagaaaatt aatgaagttc
     6241 aaacggctct gaaaaagtat atttattttc ttaaaaatat tcccctggta acttcctttg
     6301 tcaataaaaa gaaaattaat ctcttcgatt ggattttgga agtagccgcc tgtgagatag
     6361 aggaaattgt gtcgccggca actaaagaaa atctgctaat cgaaaccatg acggctttga
     6421 tgaatgagag aattcgagta atgcctgaat tggcgttaag cgacgaggaa aaactgacgc
     6481 agacttatat tgccgttcat cggactttgt ttgatttgga cgattctttg attacctacc
     6541 gcttattaaa gcgccgttat cctcaatgga ctaatccgag tgagacgttt attagcgaga
     6601 cagcagttaa tattttttcg ctctgggaaa gtttagaaaa ggaattaaat catcccttat
     6661 cgaaagattt ttttgatatt tgtgacaaaa ctgatactat ttttattctt ttgggagata
     6721 ttttggatca ttttaaagat gaaccagatc agctagaggc ggctttagcg agtgaagaac
     6781 ggttaaaagc tttattgtcc cgattttatc aaaaaaggcg ccaaacgtta aaatcccgtc
     6841 tctttaaaat cgccattttt tccagtttat cagttttaat ttccaattgt tttagttatt
     6901 ttattgtcga agtaccattg gctcttcttc tctatgacag atttagttta atagcgacca
     6961 ttgttgattt tattctgccg gccacggcca tgtttttgtt agtggcgatt atcaaaccac
     7021 cgtcagctac taattttaag aaagtagtcg aattaacttt taattttgtt tattccagtc
     7081 gggagaaaaa tatttacgaa attaaaccta aaaagaaaac cagtttatgg accacttttt
     7141 ggattgcttt attttatctt attggaggga cagttttctt tggtttagtt gcttggggtt
     7201 tttataaagc taaaattccc gtaatttcgg ttattttaga caccgtagtc attgctatta
     7261 atgtttttgc cgctctctta gttagaaata aggctaaaga gattactgtt gaagaaaaaa
     7321 caaccttttg ggaatttatt ctagacacga tttctattcc ggtagcccaa gttggctcat
     7381 ggctggcgag aaaatggaag gaatataatg tcgctgccgc cttttttaat gccgccattg
     7441 aaatgccagt aattaccatt attgggttta ttgaagactg gcggactttt ctccgtgaga
     7501 aaaaaggtag tttacattaa gttttatgag tggttttttg gtaatcttcg atagcggctt
     7561 ttagggcatc aacggccaga actgaacagt ggattttgag agccggcaga ccatcgagag
     7621 cttgaacaac ctcttctttg gtaattttcg aagcttcctc aagagttttt cctttgatca
     7681 ttgtcgttaa aattgaactg gaggcgatgg ctgaaacaca accaaaggtt tcaaaagtag
     7741 ctttggtaat agtcttgttt ttgatcttaa gataaagttt catcacatcg ccgcaaacga
     7801 tattgccaac ctggccgata ccgtcggcat ctttaatttg gccgaaattt ttcggtttgc
     7861 gaaaatactg catcactttt ttagaataca ttttagttaa gaaatggtga taatttattt
     7921 aatttttgga ctgctaatgg taatttttga atgagataat cgatgtcctt ctctttagtc
     7981 catcgtccca gactgattct aagcgaaccg tggatttcct ccttttttaa accacaggca
     8041 attaaaacat gggaaggagt taatttaggt gaagagcaag ctgatccggt agaggccata
     8101 attccatctt cgtctagtaa atagactaat gactcccctt cgatataagc aaaacggaaa
     8161 ctaacaatat taggtaatct ttgggtagga tggccgttta aactggcagt ggggatagct
     8221 ttaaggatat gttcaactaa tttgtctctt aatttagtta atttaactga ttcagtggtc
     8281 attgtctttt gggcaatagt catcgcttgg gtaaaaccaa caatagccgg taagttgaca
     8341 gtggacgatc tcataccttc ctcttggccg ccgccatgca tccacggctc gattttagtt
     8401 ccttttttaa tgtataaaag ggcggcacct ttcggtccat acattttatg ggacgaagca
     8461 gtcaagagat cagcaccgat tttttggacg tcaacaggga ttttgccgca ggtttgggcg
     8521 gcatcggtgt gaaagtaaac cccatgttga tgacagattt gggcgatttt agttactgga
     8581 ttgatcgtac cgatttcatt gttagcctgc atcacagaga ccaaaacagt ttgggaggta
     8641 attgcttttt ctacctcttc tgggtgaacc agaccatatt ggtcaaccgg tacaaaagta
     8701 acttcaaagc cattttgggc taaccactta gcgctttcta aaacacaatc atgttcaatt
     8761 tgagagacaa taagatgatt accgcgactt ctattagccc acatcgttcc ttttaaagct
     8821 agattattac tttcagtggc tgagccggta aagataattt cagtggctga ctgagccccg
     8881 ataaagccgg ccgctgttcg acgacactta tccaggaccc ggagattatc ccggcccatt
     8941 cgatgcattg aagctgggtt accaattttg gccagaaaat agggcaacat cgttttaatc
     9001 actctggggt cgaccggagt agtcgcggca taatcaagat aaatttttgg ttttttcata
     9061 aatgtgctac acttgtagta tatgaaatgc acttgtaatt gtcaagttaa atgccggctt
     9121 ttaggcagtt tggagcagga gattatggag atcctttggg cttcccctca gccgctcaaa
     9181 ccagcagtgg ttcaacagac tcttaaaaat aaatatgctt acactactgt tatgacagtt
     9241 ttaaaccgat taaataacaa aaagatagtt aatcggcggg taaaaggaaa ggcttactat
     9301 tattcacctt gtttgaaaaa agaagaattt gttcgttcct gtttacagga tttgtttgcc
     9361 cgtcttaaac tctcttatgg ctctgttttc attaaagagg cagttgacta tctactacgc
     9421 ctgtagtagt ttgaggacaa ttaaaaatta aaatttaaag aaaagaagta agttttgact
     9481 cgattagttg cagctgatcc ggatcgacac cgatgctttt gagtttagcc aaaagcatcg
     9541 gagcaaattt ttcctgccgc caaatttgtt tggtggtggc gaaatttagg tcccagaacc
     9601 agccggcatt aaacaaatac cagtcggcca gggagttaaa cgatctatca ggaaggaatt
     9661 caccatttag aaaactagct aatcttttag gactaagttg gttgcctgat aaatgaaaag
     9721 ctggaacatc atttttttcc ataaaatcaa aatctctaaa taacgcggtt ttatcggcat
     9781 ctttgaccag ccggacgtaa ttgctccggc ccggatattc tttcctcgaa tgccaaaaaa
     9841 tcgcctcgac aatttcctct ttatgaggga tatcgaagtt ttgctttagg aacatctctg
     9901 ctcccaaagt ggcgtggtcg atccctgatt gggcatcaat gaaggtgttg ctttgccgcg
     9961 cttggggaaa acggccgatg tcatgaagaa gacagataat tgttcccaag cttttgtccc
    10021 agtcaaccga tgactctttg gccattattt cccagccggc ctcaaccgtt ttgtaagtat
    10081 ggttaatttt atactctatt ttagcccgcc gttccttatt attagtctta agccaattat
    10141 ctttagtcac tgtttcaaac cagtgaataa ttggcgccgg tacggatgaa tttaaacatt
    10201 catctttccg ataaaatcga gcaattgata attcggtttt cattttaaaa gagcttgttg
    10261 ggattgaata aataaatcga aacctttatc ggttaaagga tgtttaatat taagatggcg
    10321 gtgaggagcc acgtatttaa tatttttggg ccttttgaag ttatcgcctt tccactcgaa
    10381 aagaatttga ggtggtacag taacgatatc gacttttagg gctaaacaat cataaaattg
    10441 atcttttgat ctgatactag cagctaataa tttagtttta gccttgtctt tggtaaattg
    10501 ccgctgaata ttctcaatta aactcatgcc tcttagacca atatcgttta aacggccgat
    10561 aaaaggtgaa acataaactt gatcgttttt tgataaatag ccaacgattc tttcaacagc
    10621 cgccgcctgt tctaaagaaa aacagagagt catattaacc cgaccgcccc ttttgacaaa
    10681 atcagccgcc gccgccagac cggctttaaa aatgggaatt ttaatatgaa ttcgagatac
    10741 ctgatctgac atcttctcgg cgagtcgata aatttcctct ttaggcgttt tgggggtgat
    10801 gaagatttct cccgaaatcg atccttgggg caaaatcttt ctgatttctc ttaataggtt
    10861 gatataaatc cgatctaatt cttgaggact gaattttttt tcgcccatgg ctttttgtaa
    10921 aagactggga ttagtcgtaa ctccatcaat aggtacacct tgagctaata attccttaat
    10981 taaagataaa tcagccgtgt cggcgaagat tttagtcatt aatttatctc cctaattaaa
    11041 cttttaataa aatcaaaact attttgccag aaattttctt gtttaatatt gatcccaaga
    11101 tcaaggagca gtttaaccgg atcttgacta cccccggctg ctaaaattgc ttcaattact
    11161 ttggcataat ttttgtcttt tttatattca gagtagagac ttaaacttaa taattggcca
    11221 aaattatacg cataacaata aaaaggagtt tcaaaaagat gggtgacata actccattca
    11281 taacggaagt tatcggggat aacaagatta tcaccaaatt gatcttttaa attgttaaga
    11341 tacatcg
//
LOCUS       DAOOIE010000003         9898 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_6098_length_9898_cov_3.053642, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000003 DAOOIE010000000
VERSION     DAOOIE010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 9898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9898
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6098_length_9898_cov_3.053642"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /gene="mltG"
                     /locus_tag="PK131_00270"
     CDS             <1..368
                     /gene="mltG"
                     /locus_tag="PK131_00270"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPD44606.1"
                     /translation="REGRQLETKQMIAGILLNRLKANLALQIDATVQYARDTFRNPKV
                     YWQPLQADDINLVSPYNTYKNKGLPPGPICNPGYDSLYAAFHPTASDYLFYVTGKDGQ
                     MHYAKTYQEHLENIENYLK"
     gene            complement(379..1368)
                     /locus_tag="PK131_00275"
     CDS             complement(379..1368)
                     /locus_tag="PK131_00275"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HPD44607.1"
                     /translation="MPKFYLKTFGCAQNTADSERIKAFYQHKGWQEVVDWKKADEIII
                     NSCIVRESAENRVYGLINNIRKNSVARKPKIILTGCLAAVAKQIKGVDEILPINQIAT
                     TAEPLRCRQKTALISITSGCNHFCSYCIVPYTRGREVSRPSKEILKEIDQAIKNGFKE
                     ILLIGQNVNSWNNNKFPQLLETIANRPLTKISFVSSNPWKFSDELIKVIARHNNIDRL
                     IHLPLQSGDDKILKKMNRGYTAADYLNLIEKIKSKVKNSKFSTDIIVGFPGEDEKAFQ
                     NTVKLCRRVGFEIAYINKYSPRPGTISAKIYPDNVPQTEKKRRWKILNELINK"
     gene            complement(1349..1840)
                     /locus_tag="PK131_00280"
     CDS             complement(1349..1840)
                     /locus_tag="PK131_00280"
                     /inference="COORDINATES: protein motif:HMM:NF039533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPD44608.1"
                     /translation="MKITKKVLGILFLLLISPKLTLAGGFTVSSIGGVSTNNIQVSKF
                     WHTSLQPTFRGTALPSSNVTIDIDGTAIQVAADSSGDWVYTPAASLTTGAHQVTFTNN
                     GSTQKITLTLGDLSGMDVGGISSSTSATPAAGVTWPTVLILSLGGFSIVLGLKYRHAQ
                     VLS"
     gene            2045..5311
                     /locus_tag="PK131_00285"
     CDS             2045..5311
                     /locus_tag="PK131_00285"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPD44609.1"
                     /translation="MAKRLNWGRQYPNLPELDLVAVQKQSWRRFLNCDFEEILQSISP
                     ITDYTGNNWELTLGKLSYDPILIAPQTARHKGINYALPVRIEAKLLNKRTGVETSENV
                     FLLNLPTMTEEGTFIISGIRRGIINQLVRSPGVYFTGETDPTTGKVLYNAEIRPIHGS
                     WLEFFVGKKDVIFARIDKRKKFPATVILRAACGLDDKQLIAEFGNFIESTLAADPSKS
                     AEEALIEFYKKSHPGEPVVMENAQRFFNERFFNLRSYELGRVGRFKINKKFGFNFDDN
                     NKENWVLREEDLIETVRYLIKLQKGEMTQLDDIDSLANRRLRCCGEVVAQIALRPALV
                     RFERMVKERMSLISTKEKCIPSQIINPQPLISALNTVFRSNQLSAILDQTNPLSELDL
                     LRRVTVIGQGGLSRERAAFSIRDVHSSQYGRICTVRSPEGPNIGVVTYLALYARVNDY
                     GFIEAPFRKIQKMGNDYKVTDDFVYLDANDEFNHQITHLGIKIDEKGIIRQKWVPIRY
                     RGEFIEGPIDRVDYIDLVPNQIVGVSASLIPFVDHDDATRALMGSHMQTQAVPLIKTE
                     VPIVGTGMEKPIATALGRTVKAVNSGKVTYVDGEKIVVTLDKKAKVEDGTEHSFISHN
                     GKTETYPITKFERTSPYGTCYNQQAVVNVGDRVKKGDLLIDGPATSQGELSIGRNLLV
                     AYASIDGLGFEDSFLISDRLVKEDILTNIQIYEYVAEVVETKLGSEELTKDIPNVSEA
                     ELSKLTDDGLVMIGATVGPNDILVGKVEPRGEKELSAEERLLRAIFGEKAREVKDTSL
                     RVPNGEGGVVIKVDILDKDRGDELDPGVNKVIKVYVAQIRRIQEGDKIAGRHGNKGVI
                     CRILPEYDMPYLADGTPIDLVMSPLSVIARMNLGQVLETTLAHAGLHLNKKYAVPVFG
                     KYEEKVIEAELEQAGLPTNGKMQLYDGRTGQPYDYKSVVGIGYILKLVHMVDDKVHAR
                     STGPYSLVTQQPLGGRARMGGQRLGEMEVWALESHRAAHILQEMLTIKSDDLEGRTKA
                     FQAIIKGLPIPEPAVPESFKVLTRELAGLALDVNPIGVKETQEPEEVTPTVLEEEKHV
                     "
     gene            5304..8156
                     /gene="rpoC"
                     /locus_tag="PK131_00290"
     CDS             5304..8156
                     /gene="rpoC"
                     /locus_tag="PK131_00290"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPD44610.1"
                     /translation="MFNFKNIIDFSGLQIKLASPEAVKEWSHGEVIKPETINYRSWRP
                     EKDGLFCEKIFGPSRDYECYCGKYKKIRFKGVVCDRCGVEVTTSKVRRERMGHIALAA
                     PVVHLWYLRSTPSPLSIILDIPQKEIEAVVYFTKYLVTAVDNDKRKEALVNLETNLGK
                     EYQMIEKKTKEAIEATTKSIIEEKKELKEQIKNKDQLNIAQEEQEVRLKQEIQRLENK
                     ALTEKSRLDNLTKYLKERINSITTLTTLTEEENFYLGQFKADLFYQAKMGAEAILEVL
                     ETLELSAMVVNLKKELGKTSSRLKKKKIMYKIRILNGMIENGIEPGWMVLRNIPVIPP
                     DLRPMVQLTGGRFATSDLNDLYRRLINRNNRLKKLLKLGAPEIILKNEKRMLQESVDI
                     LIDAQKSARNKRKGSKRLPRSLSDMLRGKKGRFRRNLLSKRVDYSGRSVIVVGPDLKL
                     NQVGLPKEIALEMYRPFVLRELITSGIAPNIKSAKNLVDHRVNEVYDILEKVTKKSYV
                     LLNRAPTLHKLSIQAFRPILVDGLAIRLHPCVCRGFNADFDGDQMGVYLPLSESSQKE
                     AKELMVPSRNLLKPADGSPISVPSQEMAVGCYYFTSVRSEDLEKEKDEEFKDLPVLSD
                     KTEAQLAYEAGKIGLRELIMVRINGQLLRTTYGRIWFNSILPENFEYINEPVAGSKSM
                     NDLIFKTMEKCGRLRTVHLIDDLKDIGFKGFTLSGLSLAMEDCGYLKEKDQIIAETNK
                     KIAEIEGNYKMGLITNGEKKRLCQTLWMSVTEEIAEKTWATLATDSPIRIISAAGIKR
                     ASKDQIKQLSGIRGLVVDPMGEIVQLPTKSNFREGLSVFEYVTGARGARKGLADTALK
                     TADAGYLTRRLVDAAHAAIIREKDCGTTKFLTIEKNDKGRERSFARRILGRVLASEVV
                     DPKTNKVIAKVGDIIDSRLGALIESSNVSAVNVYSP"
     gene            8204..9028
                     /locus_tag="PK131_00295"
     CDS             8204..9028
                     /locus_tag="PK131_00295"
                     /inference="COORDINATES: protein motif:HMM:NF016859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44611.1"
                     /translation="MSTRKMVKIGVPVGVIGAQSIGEPGTQLTLRTKHSGGVIGVDVT
                     QDLPRVQELFEIRTPKLPSPIAEIDGRISIKENLDNYEIKLTGKDDNGHKKIITYAVP
                     INTNLMVADGDLVIAGTQLCSGSLDVREVLNIKGLEAAQRYLINNIQFVYESQGIVIH
                     DKHIEVIIKEMSDKIKIEDPGDTNFIYGQIVTRAIYTEANDKLKEKGGEPAKGRKVLL
                     GLIQAALTTGSWLSAASFQQTTSVLTEASLLAEVDHLIGLKENVIIGRLIPVEIKN"
     gene            9042..9461
                     /gene="rpsL"
                     /locus_tag="PK131_00300"
     CDS             9042..9461
                     /gene="rpsL"
                     /locus_tag="PK131_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPD44612.1"
                     /translation="MPTFNQLIRKGRKQKPKRVKASALRGTFNSRENKRVSSFNPIKK
                     GVITVVKTMTPKKPNSALRKVCKVKLSNRASVTAYIPGVGHNLAEHGIVTVRGGRVRD
                     LPGIRYHIIRGRFDAAGVVGRKQSRSRYGTKRPGAGN"
     gene            9523..>9898
                     /locus_tag="PK131_00305"
     CDS             9523..>9898
                     /locus_tag="PK131_00305"
                     /inference="COORDINATES: protein motif:HMM:NF012404.2"
                     /note="binds directly to 16S rRNA where it nucleates
                     assembly of the head domain of the 30S subunit; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPD44613.1"
                     /translation="MSRKGRAKVRKIEPEAIYNSVTVSKIIHGAMFDGKKKVAMKQVY
                     QALAMVEKEMGDEALMLLEQALDNIRPKVEVRPRRIGGAVYQVPTPVKSNRQNSLAIR
                     WLIQSARAKANKQFHTFSEKLAD"
BASE COUNT         3037 a         1649 c         2226 g         2986 t
ORIGIN      
        1 aacgggaggg acggcaatta gaaactaaac agatgattgc cggcatttta cttaaccgtt
       61 taaaagctaa tttagctctt cagattgatg ctacggttca atatgcccgg gatacattca
      121 gaaatccaaa ggtttattgg caaccacttc aagctgacga tattaatctt gtttctcctt
      181 ataataccta taaaaataaa ggattaccac caggtccaat ttgtaacccg ggttatgatt
      241 ctctttatgc cgcctttcat cctacagcga gtgattatct tttttatgtc accggcaaag
      301 acggccagat gcattacgct aaaacctacc aagaacattt ggaaaatatt gagaattatt
      361 taaagtaaat tctaattttt atttattaat caattcattt aatattttcc accttctttt
      421 tttctctgtt tgagggacat tatcagggta tattttggca ctaattgttc ccggccgggg
      481 agaatattta ttgatatagg caatttcgaa accgacacga cggcagagtt tgactgtgtt
      541 ttgaaaagct ttttcatctt cacccggaaa gccaacgata atatcagtgg agaatttgga
      601 attttttacc ttgcttttta ttttctcaat taaatttaaa tagtcggcgg cggtgtaacc
      661 tcggttcatt ttttttaaga ttttatcatc acctgattgc aatggtaaat gaataaggcg
      721 gtcgatattg ttgtgtctgg caattacctt aataagttca tcggaaaatt tccaaggatt
      781 gcttgaaacg aacgaaattt tggtaagcgg tcggttggca atagtttcta gaagttgggg
      841 aaatttgtta ttattccacg agttaacgtt ctggccgatc aataggattt ctttaaaacc
      901 attcttaatt gcctggtcaa tttctttcaa gatttctttt gaaggtcgag aaacttctct
      961 tcccctggta taggggacaa tacagtagga acaaaagtga ttacagccac tggtaatcga
     1021 aatgagagct gttttctgtc tgcacctaag gggttcagcg gtggtggcaa tttgatttat
     1081 cggtaagatt tcatcgactc cttttatttg tttagctaca gcggccaaac agccggtgag
     1141 gataatttta ggttttcttg cgactgaatt ttttcttatg ttattaatta aaccatagac
     1201 cctgttttca gccgattctc taacaataca gctattgatg atgatttcgt ccgccttttt
     1261 ccaatcaacc acctcctgcc accccttgtg ttggtaaaat gcttttatcc gctctgaatc
     1321 ggccgtattt tgggcacaac caaatgtttt aagatagaac ttgggcatgc cgatatttta
     1381 atcccagaac gatactgaat ccacccagag aaaggattaa gactgttggc caggtaactc
     1441 ccgccgccgg cgtggcgctg gtagaactac tgattcctcc tacatccatt ccacttaaat
     1501 ctcctaaagt tagggttatt ttttgggttg agccattgtt ggtaaaggtt acctggtggg
     1561 cacccgtggt taaacttgct gccggtgtat aaacccaatc accactggaa tcggctgcga
     1621 cctgaatcgc cgttccatca atatcgatag taacattact cgaaggcaaa gccgttcctc
     1681 taaaagtcgg ttggagagaa gtatgccaaa atttacttac ctgaatatta ttggtgctga
     1741 cgccgccaat agaactgacg gtaaaaccgc cggccaaagt caatttagga gaaattaaca
     1801 agagaaacaa aatccctaaa acttttttgg taattttcat gatttaaata tatactttgc
     1861 cttgctcctc tgtcaaggag agccgtctgc cagccggcta gcagaagggt tttaattggg
     1921 tttctgacaa tctatgattt agttgttgct ttgacttgac atgggcgtta taatacaagg
     1981 catttggaag gctgaaaaga gcctttctct tttaataaaa cttcaaaatt aaattttaat
     2041 gagcgtggct aaaaggctta attggggtag acagtatcca aatcttccgg agcttgattt
     2101 agtggcagtg cagaaacagt cttggcggcg cttcctcaat tgtgattttg aggaaatact
     2161 ccagtcaatt tcacctatta ctgattatac tggtaataat tgggaattaa ctttgggtaa
     2221 attaagttat gatccaattc taattgctcc ccaaacagct cgacataaag gtattaatta
     2281 tgctcttccg gtaagaatag aagccaaact tcttaataaa agaacaggag ttgagacaag
     2341 tgagaatgtt tttcttttaa atctacccac catgacggaa gagggaacct ttattatcag
     2401 tggaattaga aggggcatta ttaatcaatt agtccgttct cccggggttt atttcactgg
     2461 agaaactgat ccgactactg gtaaagtcct ttataatgct gaaattagac cgattcatgg
     2521 ttcatggctg gagttttttg ttggcaagaa agacgttatt tttgccagaa ttgacaagag
     2581 aaaaaaattt ccggctaccg ttattttaag ggctgcctgt ggtttagatg acaaacaatt
     2641 aatagcggag tttggtaatt ttattgaaag tacattggca gccgatccgt ctaaatcggc
     2701 tgaggaagct ctgattgagt tctataaaaa gtcacatcct ggtgagccgg tagtgatgga
     2761 aaatgctcag agatttttta acgaaaggtt ttttaacttg cgaagttatg agttaggacg
     2821 ggttggtcgg tttaaaatta acaaaaaatt tggttttaat tttgatgata ataataaaga
     2881 aaattgggtt ttaagagaag aagatttaat tgaaacggtt cgttatttga ttaagttgca
     2941 gaaaggagag atgacacaat tagatgatat cgatagttta gccaatcgcc gtctccgctg
     3001 ttgtggagag gtagtggctc aaattgctct ccggccggca ctggttcgtt ttgaaagaat
     3061 ggttaaggaa agaatgtcct taatttcaac taaagagaaa tgtattccca gtcaaataat
     3121 taatcctcaa ccacttattt cagccctgaa taccgttttt cgttccaatc aattatcggc
     3181 tattttggat caaactaatc ctttatcaga attggattta cttcgtcggg taacagttat
     3241 cggccagggt ggtctttcac gagaaagagc cgcttttagt attagagatg ttcattcaag
     3301 ccaatatggt cgaatttgca ccgttcgttc acctgagggt cctaatattg gtgtagtaac
     3361 ttatttggcc ctttatgctc gggtgaatga ctatggtttt attgaggctc cctttagaaa
     3421 aattcaaaaa atgggtaatg attataaggt gaccgatgat tttgtctacc ttgatgctaa
     3481 tgatgaattt aatcatcaga ttactcactt gggaattaaa attgatgaaa aaggtataat
     3541 tcggcaaaaa tgggtgccaa tccgttatcg gggcgagttt attgaaggtc caattgatcg
     3601 ggtagattat attgatttag tccctaacca aattgtggga gtttctgctt ccttaattcc
     3661 ctttgttgat catgacgatg ccactcgggc tttaatgggt tctcatatgc agacacaggc
     3721 agttcctttg attaaaactg aagttccaat tgtcggaacg ggcatggaaa aacctattgc
     3781 tactgcttta ggacgaacag ttaaggcagt taattcagga aaagtaactt atgttgatgg
     3841 cgaaaaaata gtagtcactt tggataaaaa ggccaaagtt gaagatggga cagagcacag
     3901 ttttattagc cataacggta aaacagaaac gtatccgatt actaagtttg aacgaacttc
     3961 accttatgga acctgttata accaacaggc agtagttaat gtgggcgatc gggttaaaaa
     4021 gggtgatctt ttaattgatg gtccggcgac ttcgcaagga gaattatcga ttggtcgaaa
     4081 tttgttagtt gcttatgctt ccattgacgg tttaggtttt gaagattctt tcttaatttc
     4141 tgaccgatta gtaaaggaag atattttaac caacattcaa atttatgaat atgtagctga
     4201 ggtggtagaa accaaacttg gttctgaaga attaaccaaa gatattccca atgtgtctga
     4261 agcagaatta tctaagttaa cagatgatgg tttagtaatg atcggagcta ctgttggccc
     4321 caatgatatt ctggtaggta aggtggagcc gcgaggagaa aaagagttat cagccgaaga
     4381 aagattatta cgggcgattt ttggagagaa agctagggag gttaaagata cttctcttcg
     4441 agttcctaat ggtgaaggag gggtggtaat taaagttgat attttagata aggaccgagg
     4501 tgatgagtta gatccggggg tcaataaggt aatcaaagtt tatgtagccc aaatccgccg
     4561 aattcaagaa ggtgataaaa ttgctggtcg tcatggaaac aaaggtgtta tttgccgcat
     4621 tttaccagaa tatgatatgc cttatttagc cgatggtact ccgattgact tagtaatgtc
     4681 acccttgtca gttattgcca gaatgaattt aggtcaggtt ttagaaacta ctttggctca
     4741 tgccggtctt catttgaata aaaaatatgc tgttcctgtc tttggaaaat atgaggaaaa
     4801 agttattgag gcagaactgg aacaggcagg actgccaacc aacggaaaaa tgcaacttta
     4861 tgatggtcga actggtcagc catatgatta taaatcggtg gtaggtatcg gatatatttt
     4921 aaaattagtt cacatggtgg acgataaggt gcacgctcgt tctacaggtc cttattcttt
     4981 ggtaactcaa cagcctctag gtggccgggc aagaatgggg ggacagcgat tgggagaaat
     5041 ggaagtttgg gctttagaat cccatcgagc ggcccacatt cttcaggaaa tgttaaccat
     5101 taaatctgat gatttggaag gccgaactaa agctttccaa gctatcatta agggactacc
     5161 tattcccgag ccagcggtgc ctgaatcgtt taaggtatta acgcgggaat tagctggttt
     5221 agccttggat gtcaatccca tcggagttaa agagacccaa gaacccgaag aggttacacc
     5281 gacagtttta gaggaggaaa aacatgttta attttaaaaa tattattgat ttttctggtt
     5341 tacagattaa attagcttca ccggaagcgg taaaagaatg gtcccacggg gaggtaatta
     5401 aacctgaaac aattaattat cgctcttggc ggccggaaaa agacggacta ttttgtgaaa
     5461 aaatttttgg accatcccga gattatgaat gttattgcgg caagtataaa aagatccgat
     5521 ttaagggagt tgtttgtgac cgttgtggtg ttgaagtgac cacttccaaa gttcgtcggg
     5581 agcgaatggg acatattgcg ttagctgctc cagtagttca tttatggtat ctaagaagta
     5641 ctccttcacc tttaagtatt attttagata ttccccaaaa agagattgaa gcggttgttt
     5701 attttactaa atatttggtt acggcggttg ataacgataa aagaaaagaa gctttggtta
     5761 atttagaaac aaatttaggt aaggaatacc aaatgattga gaaaaagacc aaagaagcca
     5821 ttgaagctac taccaagtcg ataattgaag aaaagaagga attaaaggaa cagattaaaa
     5881 ataaagatca gttaaacatt gctcaggaag aacaagaggt tcgccttaag caggagattc
     5941 agcgtttaga aaataaagcc ttgacagaga aaagccggtt ggacaattta accaaatatc
     6001 tcaaggaaag aattaatagt attaccactc taactacttt aaccgaggaa gaaaattttt
     6061 atttgggaca gtttaaagcc gatctttttt atcaggcgaa aatgggagct gaagctattt
     6121 tggaagtttt ggaaacatta gaattatcgg caatggttgt aaatctaaag aaggaattgg
     6181 gcaaaacctc ttcccgtttg aagaaaaaga aaattatgta taaaattcgc attttaaacg
     6241 ggatgattga gaacggtatt gagccagggt ggatggtatt gcgaaatatt ccagtgatac
     6301 cgcctgatct gcggccaatg gtgcagctaa ctggcggtcg ttttgcgact tctgatttaa
     6361 acgatttgta tcgccgttta attaatcgga ataatcgtct caaaaaatta ttgaaattag
     6421 gagcaccgga aataattctt aaaaatgaga aaagaatgct tcaagaatcg gtcgatattt
     6481 taattgatgc tcaaaagagt gctaggaata aacgcaaggg cagtaaacgt ttacctcggt
     6541 cattgtctga tatgttgcgg ggaaagaaag gacgttttag aagaaatctt ttaagtaaac
     6601 gggttgatta ttccggccgt tcggtcattg ttgtcggtcc ggatttaaaa ctcaatcaag
     6661 ttggtttacc taaagagatt gccctggaaa tgtatcggcc ttttgttctt agagaattaa
     6721 ttaccagcgg aattgccccc aatattaaaa gtgctaaaaa tttagttgat catcgagtca
     6781 atgaggttta tgatattttg gagaaagtta caaagaaaag ttatgttttg ttaaatcggg
     6841 cgccaacact ccataaactt tccattcaag cctttagacc gattttggta gatggtttag
     6901 ctattcgtct tcacccctgt gtttgtcgag gttttaatgc cgattttgac ggcgatcaaa
     6961 tgggagttta tttacccctc tcagaatcat cccaaaagga agctaaagag ttgatggtac
     7021 cttctcgtaa tctcttaaag ccagctgatg gttcgcctat ttctgttccc tcgcaagaaa
     7081 tggcggtggg ttgttactat tttacttcgg ttagatcgga agatttagaa aaagaaaagg
     7141 acgaagagtt taaagatttg ccggttttaa gtgataaaac agaagctcag ttagcctacg
     7201 aagcaggtaa aatcggtttg agggaattaa ttatggtacg aattaatggt caacttttaa
     7261 gaactactta cggtcggatt tggtttaatt ctattttacc ggaaaatttt gaatatatta
     7321 acgaaccggt agctggaagc aaatcaatga atgatttgat ttttaaaaca atggagaaat
     7381 gtggccgcct gcggacagtt catctgattg acgatttaaa agatataggt ttcaagggtt
     7441 ttaccctttc tgggctgtcg ttggcgatgg aagattgtgg gtatttaaag gaaaaggatc
     7501 aaattattgc tgaaactaat aaaaaaattg ccgaaattga gggaaattat aagatgggct
     7561 taattacgaa tggggagaaa aagcgtttgt gtcagacttt atggatgagt gtaactgagg
     7621 aaattgccga aaaaacatgg gcgactttag ctaccgattc acctattcgg attatctcag
     7681 ccgcaggaat taagcgggct tcgaaggatc aaattaagca actttcaggt atacggggat
     7741 tagtggttga tccaatgggt gaaattgttc aactgccaac taaatctaat tttcgtgaag
     7801 gtttatcggt ttttgagtat gtaactgggg cacggggagc aagaaaaggt ttggccgata
     7861 cggccttaaa aacggctgac gccggttatt taactcgccg gttggttgac gccgcccatg
     7921 ctgcaattat tagggaaaaa gattgcggaa ccaccaagtt tttaaccatt gaaaaaaatg
     7981 ataaaggtcg agagcggtct tttgcccggc gaattttggg ccgagtttta gcgtcagaag
     8041 tagttgatcc aaagacgaac aaggttatag ctaaagtcgg tgacataatc gattctcgat
     8101 taggagcctt gattgaaagc agcaatgttt cagcagttaa tgtttattca ccttaacttg
     8161 tcgatcttta aatggtcttt gtcaaaaatg ttatggttgg gatatgtcaa cccgtaaaat
     8221 ggtaaaaatc ggagttccag taggagttat cggagcccaa tctattggtg aaccgggaac
     8281 ccagttaact ttaagaacta agcatagcgg gggagtaatc ggagtcgatg ttactcaaga
     8341 tttacctagg gttcaagaat tattcgagat aagaactccg aaattgcctt cgccaattgc
     8401 ggagattgat ggccggattt caattaaaga aaatcttgat aattacgaga taaaattgac
     8461 aggcaaagat gataatggcc ataagaagat tattacctat gcagttccca ttaatactaa
     8521 tcttatggtg gccgacggcg atttagttat tgccggtact cagttatgtt cgggttcctt
     8581 ggatgtgcgg gaggtcttaa atattaaagg tttggaagca gcccagcggt atctgattaa
     8641 taatattcag tttgtttacg aatcccaggg gattgttatt cacgataaac atatcgaggt
     8701 gattattaaa gaaatgagtg acaaaataaa aattgaagat ccgggtgata ctaattttat
     8761 ttacggtcag attgttaccc gggcgattta taccgaagcc aatgataaat tgaaagaaaa
     8821 gggcggtgaa ccagctaaag gtcgaaaagt tcttttgggt ttgattcaag cggcccttac
     8881 cactggttct tggttgtcgg cggcttcatt ccaacaaact acctctgttt taaccgaagc
     8941 ttcattatta gccgaagtgg atcacttgat tggtttgaag gaaaatgtta ttatcggcag
     9001 attaattcca gtagaaatta aaaattagaa attagtttag agtgcctaca tttaatcaat
     9061 taattagaaa aggacgaaaa caaaaaccta agcgggttaa ggcatcggct cttcggggta
     9121 cttttaatag ccgagaaaac aagcgggttt ccagctttaa tccgattaaa aaaggggtaa
     9181 ttacggtagt taaaactatg acgcctaaaa aacctaattc agccttacgt aaagtttgta
     9241 aagtaaagtt aagtaatcgg gcttcagtaa cagcttatat tcccggcgtt ggtcataatt
     9301 tggctgaaca cggtattgtt accgttcgcg gtggccgggt tcgtgatttg cccggtattc
     9361 gttaccatat tatccgaggt cgttttgatg ccgctggagt ggttggtcgg aaacaaagcc
     9421 gttcccgcta cgggactaaa cgcccgggag ccggtaatta attacgaatt aaaaattaaa
     9481 aatctcgaga attttaatta ttttaaattt taaattaagc ttatgtcacg taaaggtcga
     9541 gctaaagtaa ggaaaataga gccggaggcg atttataatt cggtgacagt tagcaaaata
     9601 attcatggag ctatgtttga cggtaagaaa aaagttgcca tgaaacaggt ctatcaagcc
     9661 ttggccatgg tggaaaaaga aatgggtgat gaagcgctaa tgttattaga acaggccttg
     9721 gataatattc gaccaaaagt ggaagttcga ccgcgtcgta ttggtggagc agtttatcaa
     9781 gtaccgacac cggtaaaaag caatcgccaa aattctttgg cgattcggtg gttaattcag
     9841 tccgctcgag ctaaagctaa taagcaattc catacctttt cagaaaaatt ggctgatg
//
LOCUS       DAOOIE010000004         9534 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_6338_length_9534_cov_3.488976, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000004 DAOOIE010000000
VERSION     DAOOIE010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 9534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9534
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6338_length_9534_cov_3.488976"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PK131_00310"
     CDS             complement(<1..392)
                     /locus_tag="PK131_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPD44614.1"
                     /translation="MNKSEIKYKEAEFYEQLEEGKVKCQLCPNNCLLKNKQVGLCKVR
                     QNFNGRLYSLVYGRAAAVNLDPIEKKPFFHFFPGEKAFSLGTAGCNLHCLNCQNWDIA
                     QRSPKDIPAIELTPAEIIQKTKASGAKI"
     gene            complement(461..1054)
                     /locus_tag="PK131_00315"
     CDS             complement(461..1054)
                     /locus_tag="PK131_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPD44615.1"
                     /translation="MKEIAVGFFGGLSVGVYCLGSCLPVFLPILLAEKRSLKKSFGIL
                     WEFSLGRLLGYLIFGVIIGYLGQKINNTWIHAGALIITLWMGIIMILFSLNKIDRRYC
                     LMSPWKRIKTAFLIGFLTGVNVCPPFLGSLAYVFNLRDALKAAIYFLAFFVGTSVYLL
                     PATFLGFTSKFKLMRQIAQFAGLGIGVYFVIKSLLSL"
     gene            complement(1192..2535)
                     /gene="amrB"
                     /locus_tag="PK131_00320"
     CDS             complement(1192..2535)
                     /gene="amrB"
                     /locus_tag="PK131_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPD44616.1"
                     /translation="MKWLITIFILLLGGLIMFKTNQKNIRPAAVAGSFYPADKNILAK
                     ELDGYLNNAQKTGKDYRVLIVPHAGLVYSGRTAGYGFKQIEDRDFQRVILIGPSHQVY
                     LNEAVTSGADYWETPLGRTAVEKSDFTINDKAFAQEHSLEVELIFLQRILKNFKIIPI
                     LINEYTDISDKIIKLMDDNTLLVVSSDLSHYHPYNEAQKLDQKTIESIINQGKEIDAC
                     GRAAIEAAMAIAQKMGIKFEKIYAENSGDTSGDKSQVVGYAAIGGYRETSPVEDWQKE
                     ALTIARKTLEEYLHNGEKLKFKILNSKLNEKLGAFVTLRKNGQLRGCIGLFEPNLPLY
                     QVIQQMAIAAATEDPRFYPVSYDELKEIKIEISVMTPKKLIDDYHKIELGKHGVVIER
                     GGRSGTFLPQVATETGWNLDEFLGELCSQKAGLPRNCYRDSQTKIYTFEAEVFEE"
     gene            2534..4000
                     /locus_tag="PK131_00325"
     CDS             2534..4000
                     /locus_tag="PK131_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44617.1"
                     /translation="MPFLYFTLGLISVVTQSFLIRLAQVYLFHTDLGYGIFLSFWLFF
                     SGLGCFSPKIYPFYLIPPYLFGVIYNYFFKYLKQANQAYIYETLGFCFGGLLFGLGVS
                     RIGSPYQKVTIKHYQSQDQIFTNNRPYYSFPASEDAYVLKYLSLTSFSKVLLKANPNY
                     AHTLSQISPQAQIDLLEVDPVLRKIGQKYLPANACFVSQPDLFPQQAGNYDLVILAQD
                     IPRSLSDNRYYTREFFQTIKSKILIIFPYRDFAAPAYYYPIASILSALPSAKSYLVDN
                     YLLIANYDLGLQPITFPVQANPNSRRQPQAFFLAQIYHLSSLWPKLPIIIKITALIFP
                     LILLFFLTSKNDLKSIFGASFVSFAFNTLALYHFQEIYGSLYSQISLLLALNLFCLSL
                     GVYLSKNFSRPPFLLFFLILVSFHPIITMIIASLTYGFCFGVLNRQPITSPYAFDSWG
                     AALGSLIVTTVLLPIWGINYIVLFCLVLTLFLFFRSLN"
     gene            complement(3993..4778)
                     /locus_tag="PK131_00330"
     CDS             complement(3993..4778)
                     /locus_tag="PK131_00330"
                     /inference="COORDINATES: protein motif:HMM:NF021389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2090 domain-containing protein"
                     /protein_id="HPD44618.1"
                     /translation="MKNFSKLYLLPFDHRSSFEKLVDKEKIPEAKMIIYEAFLQIREN
                     YPHPEFLGILVDNTYGRMILDDAQTKGITRAVCIEKSGQPLFDFDQENWMEILDEIKP
                     EIIKVLVRYNPEEKENNSIQLEKLAKVFDYCQSKNLPLLFELLTGEAGKTATAIKEIK
                     QKLQPTIWKLEGVEAGQWEEILAVTQEDKIIVLGRGENADKVKQWIMEAKKYPQIIGF
                     AIGRTVFLEPIQKWVNGELNHNQARDQISQNYQSWIKLWEEIN"
     gene            4797..5243
                     /locus_tag="PK131_00335"
     CDS             4797..5243
                     /locus_tag="PK131_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44619.1"
                     /translation="MLIRPVYANCPACIVTVGGGLFLAEKLHIDVFLISIWLSALNSA
                     IAYFLASKIKRKVLNQGFFWSLIFYLLTVVYLDFSGRLRNSNPIWGIDRCLLGLSCGL
                     ILFIFSIYLDQLLRRLNRGKVVFFYQKVIIPLLVLTAATIMFKQWL"
     gene            5234..5770
                     /locus_tag="PK131_00340"
     CDS             5234..5770
                     /locus_tag="PK131_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44620.1"
                     /translation="MAVNPEEVSKLINKVKSLEKGHRLDLSSGEDLSVAIMNLIGIEE
                     HMFFTAQKTGQVEYLTVLQQAREMRKELMKKIVKDNKGEVWCTSKHLLAASMRLMEVG
                     TKCLTQKKKKEAQDYFQKSFTLYNLFWGLNLGLVKDGSQALKIEIDKSPAEQPLPKKD
                     LLGNISRIIGKILNCCRE"
     gene            5763..6236
                     /locus_tag="PK131_00345"
     CDS             5763..6236
                     /locus_tag="PK131_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPD44621.1"
                     /translation="MNKFTFKKGITESQIDELIFYANNDPQIISFTSDKTRFADKKAF
                     KLWLKKKPVIYTLTNNTGRLLGIIWFQRKIGIPGYDLTFAIRLYGKARGKGLSYGFMK
                     QAFDDFKPRNVWLKCLADNLPAVTLYKKFGFQQITNPDKNGKIIMVRKSRPNQSS"
     gene            complement(6196..6681)
                     /locus_tag="PK131_00350"
     CDS             complement(6196..6681)
                     /locus_tag="PK131_00350"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPD44622.1"
                     /translation="MENKTDEYYIKKCIKLAEKSVNNGEAPFGSLIVQNGKIIAAATN
                     ANEKRHDVTSHAEIMAMRLAQRKLNSKDLKDCEIYSNCEPCAMCSFMIRELHFKKVVF
                     AVHSPNMGGYSHWKILKDKKLAKIKPFYTEPPQVKGGILEKEAKETFKKIGWGGFFEP
                     L"
     gene            6764..7726
                     /gene="glpX"
                     /locus_tag="PK131_00355"
     CDS             6764..7726
                     /gene="glpX"
                     /locus_tag="PK131_00355"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HPD44623.1"
                     /translation="MEKFIADHFPNVTEVGAMAAARVVGLGDKMAADRAAVQAIRGAF
                     DEIDFNGRVVIGEGERDEAPMLYIGEKVGSGQGHQIDIAIDPLENTNATANFANNAIS
                     VLAASEKGGLFHAPDIYMNKLIVGEQAAGKVDLDAKPAENLKILAEALKREIRDLVVV
                     ILDRERNEELIARVREAGARVKLVADGDLMPGVAVCLRGSGVHAVMGIGAAPEGVISA
                     AAVSCLKGEFLGRFWPKNSQEEKKLIQMGGNLAKIYNQAELASGENLVFCATGITGGD
                     LLEGVRFFGGGAKTQTLVLAKKTGKVRFITTVHVLDRNEIEFRL"
     gene            7726..9462
                     /locus_tag="PK131_00360"
     CDS             7726..9462
                     /locus_tag="PK131_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD44624.1"
                     /translation="MKNALRTYYSFLWRYKTYFLLFFFCLILNAIAWSLSPYFYKLFL
                     DNFASTDLSLVIKILIIFALVRIIAVLSEVGAGIAGDKMLVPASRDARLKFFQRINEL
                     DFAYHLSQKTGSLISTVKRGDGAFINLHFMLHWQVGSILINFVVMLFFLIRLNFWLLI
                     AVLFAFILGLITARQLIRQNTQARREFIQSDDRVMDVITDNLMNYETVKLFAKEKWEL
                     FRLGEIHYDWMKKFRRYNWSFRKIDITTGMINNLGLLIVLVLGLIQLRSQIISLGDYM
                     MVLGFMTSFYPRFFDLIYSARHIIQSGVDIEKYFQIFDEPVLVPDPIKPVKRDFIIGE
                     IEFRRVTFTYPDNKRPALKDFSLHIHPGQAIALVGKSGVGKTTLGRLILRFYDVQKGQ
                     ILIDGINIKRFSKDQLRSFIGVVPQEPILFNDTIGYNIAYGRAKASQEEIIAAAKMAN
                     LDEFINGLPQGYDTLVGERGVKLSGGQRQRLAIARMILSNPSIVIFDEATSQLDTESE
                     KKIQSAFWQAVKNKTTIIIAHRLSTVINADKIVVLHQGQIAEIGSHQELIQKTNSLYS
                     YFWHLQTQSEIL"
BASE COUNT         2642 a         1701 c         1847 g         3344 t
ORIGIN      
        1 atgattttgg cgccgctggc cttagttttt tggataattt cggccggggt taattcgatt
       61 gcagggatat ctttaggtga gcgttgagca atatcccaat tttggcaatt aagacagtga
      121 agattacaac cggcagttcc caaagaaaag gccttttctc ccggaaagaa gtgaaaaaac
      181 ggtttttttt cgatcggatc aaggttaact gccgccgccc ggccgtaaac taaactatat
      241 aagcggccgt tgaaattttg tctgacttta cacagaccaa cttgtttatt ttttaataga
      301 cagttattag ggcaaagctg gcattttacc ttaccctctt ctagttgttc ataaaattct
      361 gcctctttat actttatttc gcttttgttc atcctcatga taattataaa tgaaaaaata
      421 attcccaaac tgataataat taagattggt agtttctttt tcacaggctt aaaagacttt
      481 tgataacaaa atagacccct atgcctaagc cggcaaattg ggcgatttgt ctcattaatt
      541 taaatttgct ggtaaatcca agaaaagtag ccggtaggag gtaaacactg gtaccgacaa
      601 agaaagctaa aaaataaata gccgctttaa gagcgtcgcg gagattgaag acataagcta
      661 gcgagccaag aaaaggcgga caaacattga ccccggtcaa aaatccgata agaaaggcag
      721 tttttattct tttccacggc gacattaaac aataacgacg gtcaatttta ttgagggaga
      781 aaagaatcat aatgattccc atccataaag tgattattaa agcgccggca tgaatccagg
      841 tgttattgat tttttgacct aaatagccga taataacgcc gaaaattaga tagccgagca
      901 aacggccgag actaaattcc caaagaattc caaaactttt tttaagactt cttttttctg
      961 ccagtaaaat cggtagaaaa actggcagac acgaaccgag acaataaaca ccaactgata
     1021 atccgccgaa aaaaccaacg gcaatttctt tcattattcc ccctcctccc aatttttagg
     1081 gcgagtattt tgttctaaat ttttaataat aatgttgacg gtaatttctt tcattatatc
     1141 ccgttctaat tttttggtga tggttttaac cgcatttttt cattttattt tttactcttc
     1201 aaaaacctcg gcctcaaaag tataaatttt ggtttgagaa tctcgatagc aattccgagg
     1261 caggccggct ttttgggagc ataattcgcc taaaaactcg tctaaattcc aaccggtttc
     1321 tgtcgctact tgagggagaa aagtgccgct gcggccgccc ctttcgatga ccaccccgtg
     1381 ttttcccaat tcgattttat ggtaatcgtc aattaatttt ttgggcgtca tcactgaaat
     1441 ttcgatttta atttctttca attcatcata actgacaggg taaaatcgag gatcttcggt
     1501 agcagcagct attgccatct gttgaatgac ctgatagagg ggaagattag gttcaaataa
     1561 accgatacag cccctcaact ggccattttt tcttagagtg acaaatgccc ctaacttttc
     1621 gtttaatttt gaattcaaaa ttttaaattt taatttttct ccattatgta gatattcttc
     1681 cagcgttttt cgggcgatag ttagagcttc cttctgccaa tcttcgactg ggctggtttc
     1741 ccgatagccg ccgatggcag cataaccgac tacctggctt ttatcaccgc tggtatcacc
     1801 cgaattttcg gcgtaaatct tctcgaattt tatccccatt ttttgggcta tagccatggc
     1861 ggcttcaatg gccgctcgac cgcaggcgtc aatttctttg ccctggttga tgatagattc
     1921 gattgttttt tggtccagct tttgggcttc gttataggga tgataatgag ataaatcgga
     1981 actgactact aacagagtgt tgtcgtccat taatttgata attttatcgc taatatcggt
     2041 atattcatta attagaatag gaataatttt aaaatttttc aaaattcttt ggagaaaaat
     2101 tagttctact tccaaagaat gttcttgagc aaaagcttta tcatttatcg taaaatcact
     2161 tttttcgacc gctgttcgac ccaatggtgt ttcccaataa tcagcgccgc tggtaacggc
     2221 ctcgttaaga taaacctgat ggcttgggcc gataagaata accctttgaa aatcacgatc
     2281 ctcgatttgt ttgaagccat agccagcggt tcggccggaa tagactagac cggcgtgggg
     2341 gacaatcaaa accctgtaat ctttgcctgt tttctgggca ttgtttaagt agccgtctaa
     2401 ttctttcgcc agaatattct tgtcggccgg ataaaaactg ccggcaactg ccgctggcct
     2461 gatatttttt tgattggttt taaacataat tagacctcct aagagtaaaa taaaaatagt
     2521 tattagccat ttcatgcctt tcctttattt tactctaggt ttaatttcgg ttgtaaccca
     2581 gtcattttta attcgcctgg ctcaagttta tctttttcat accgatttgg gttatggcat
     2641 tttcctttcc ttttggctct ttttttctgg tcttggctgc ttttctccta aaatttatcc
     2701 tttttatctt atccccccgt atctttttgg ggttatttat aattattttt ttaaatattt
     2761 gaagcaggct aatcaggctt atatttatga aactcttggt ttttgttttg gtggtttact
     2821 gtttggttta ggagtcagcc ggattggttc accttatcaa aaggtaacga ttaaacatta
     2881 tcagagtcag gatcagattt ttactaataa ccggccttat tactcctttc ctgcctctga
     2941 agacgcttat gttcttaaat acttgtcttt aacctcattt tccaaagttt tattaaaagc
     3001 taatcctaat tatgcccata ctttaagcca gatatctcca caggctcaaa tcgatctttt
     3061 ggaagttgat ccggttttgc gaaaaattgg gcaaaaatat ttgccggcta atgcctgctt
     3121 tgtctctcag ccggatttat tcccccagca ggcaggtaat tatgatttag ttattttagc
     3181 ccaagatatt cctcgtagtt tgtcggataa tcgctactac actcgagaat tttttcaaac
     3241 gattaaaagt aaaattttga ttatttttcc ttaccgggat ttcgccgccc cggcttatta
     3301 ttatccgatc gcgtctatat tatcagcgtt accttcggcc aaatcatatt tggtagacaa
     3361 ttatttacta attgctaatt atgatttggg attgcaacca ataacttttc ctgttcaggc
     3421 taatcctaat tcccgccgtc agcctcaagc cttttttctg gcccaaattt accatttaag
     3481 ttcattatgg cctaaattac ctataattat taaaataact gccttaattt ttcctctaat
     3541 ccttcttttc tttttaactt ctaagaatga tttaaaaagt atttttggag cctcttttgt
     3601 ttcttttgcc tttaatactc tggctcttta tcattttcag gaaatttatg gtagtttata
     3661 cagccaaatt agtttacttc tagccttgaa tttattttgt ctatctttgg gtgtttattt
     3721 gtctaaaaat ttttctcgtc ctccttttct tcttttcttt ttaattttgg tttcttttca
     3781 cccgataata acaatgatta ttgcttcatt aacctatggt ttttgtttcg gtgtgcttaa
     3841 tcgacagccg attacctctc cttatgcttt tgattcttgg ggagccgctc tcggttctct
     3901 aatcgttact actgttcttt taccgatctg gggaataaat tatattgtat tattttgttt
     3961 ggtattaacc ctatttcttt tctttcggtc acttaattaa tttcttccca caatttaatc
     4021 caactttggt aattttgact aatttgatct cgagcttgat tatggttaag ttcgccgtta
     4081 acccattttt gaatcggttc taaaaagact gttcggccaa tagcaaaacc gataatttga
     4141 ggatattttt tagcctccat aatccactgc tttaccttat ccgcattttc tccgcggcct
     4201 aaaacaataa ttttatcctc ttgggtaaca gccaaaattt cttcccattg acctgcttcc
     4261 accccctcca gcttccaaat ggtcggttga agtttttgtt taatttcttt gatggctgta
     4321 gccgttttac cggcttcacc ggttaataat tcgaaaagca gcggaagatt ttttgattga
     4381 caataatcga aaactttggc tagtttttcc aattgaatag aattattttc tttttcctcc
     4441 ggattgtatc tgactaaaac cttgataatt tcaggtttaa tttcatcgag aatttccatc
     4501 caattttctt ggtcaaaatc aaaaagcggc tgaccgcttt tctctataca aacagcccgt
     4561 gtaattcctt tagtttgagc atcgtctaaa atcattcgac cgtaggtatt gtcaactaag
     4621 atacctaaaa attctgggtg ggggtaattt tcccgaatct gtagaaaagc ttcataaatt
     4681 atcattttgg cttctggaat tttttcctta tctaccagtt tttcaaatga agaacggtga
     4741 tcgaagggga ggaggtagag ctttgaaaag tttttcatct tggtttatta taaagcatgt
     4801 taattaggcc tgtctatgct aattgccccg cttgtattgt cactgtcggt ggaggacttt
     4861 ttttggcaga aaaacttcat attgatgttt ttttaatatc gatttggctg tcggctttga
     4921 attcggctat tgcctatttt ttagcatcaa aaatcaaaag aaaagtttta aatcaaggtt
     4981 ttttttggtc gcttattttt tatcttttga ctgtagttta tcttgacttt agcggccgat
     5041 tgagaaattc taatccaatc tggggaatag atcgttgtct tttagggctg tcctgtggtt
     5101 taattttatt tattttttct atttatctcg atcaactgct ccgccgttta aatcgtggta
     5161 aagttgtatt tttttatcaa aaagttatta tccccttgct ggtcttaacg gcggcaacta
     5221 taatgtttaa gcaatggctg taaacccgga agaagtaagc aaactaataa ataaggttaa
     5281 gagtttggag aaaggtcatc gtttagattt atccagtggc gaggatttat cagtggcaat
     5341 catgaattta attgggattg aagagcatat gttttttact gcccaaaaaa ccggccaagt
     5401 tgaatattta acagttcttc aacaagcccg ggaaatgcgt aaggaattaa tgaaaaaaat
     5461 agttaaagat aataagggtg aagtttggtg caccagcaag catttattag cggcttcgat
     5521 gagattaatg gaagtgggaa ctaagtgttt aactcaaaaa aagaaaaaag aagctcaaga
     5581 ttattttcaa aaatctttta ccctttataa tcttttttgg ggattaaatt tgggtttagt
     5641 taaagatggt tctcaggcat taaaaattga aattgataaa tcgccggcgg aacagccttt
     5701 gcccaaaaag gatctactgg ggaatattag tcgtattata ggtaaaattt taaattgctg
     5761 ccgtgaataa atttactttt aaaaaaggta ttaccgaatc tcaaatagat gaattgattt
     5821 tttatgccaa taatgatccc caaattattt cttttacttc tgataaaact cgctttgccg
     5881 ataaaaaagc ttttaaactg tggcttaaga aaaagccagt tatttatact ttgaccaata
     5941 ataccggtag actgctgggt attatttggt ttcagagaaa aattggaatc cccggttatg
     6001 atcttacatt tgcgattcga ctctatggta aagctagagg taaaggactg agttatggtt
     6061 ttatgaagca agcttttgat gatttcaaac cgcgaaatgt ttggctgaaa tgtttggctg
     6121 ataatttacc ggcagtaact ctttataaaa aatttggttt tcaacaaatt accaatcctg
     6181 acaagaatgg caagattata atggttcgaa aaagccgccc caaccaatct tcttaaaagt
     6241 ttctttggct tctttttcta aaataccgcc tttaacttga ggtggctcag tataaaatgg
     6301 cttaattttg gctaattttt tgtcttttaa aattttccaa tgggagtaac cgcccatatt
     6361 gggagaatga acggcaaaaa ccactttttt aaaatgaagt tccctgatca taaaggaaca
     6421 catggcgcag ggttcacaat tggaataaat ctcacaatct tttaagtctt tggagtttaa
     6481 ttttctttga gctaaccgca ttgccataat ttcagcatga gaagtgacat catgtctttt
     6541 ttcgttagcg ttggtggcgg cagcgataat tttgccgttt tggacaatta aactgccaaa
     6601 gggggcttcc ccattgttga ctgatttttc agctaattta atacattttt taatgtagta
     6661 ttcatcggtt ttgttttcca taagctttgc tgtaattata caactttcaa taaatccgct
     6721 ctaattaact attaattcaa gagtcatttt attataattg ttcatggaaa agtttattgc
     6781 ggatcatttt cccaacgtaa ctgaagttgg ggcaatggcg gcggcccgag ttgtaggttt
     6841 gggagataaa atggcagctg accgggcagc cgttcaggcg attcgggggg cctttgatga
     6901 aattgatttt aatggccgag ttgttattgg tgaaggtgag agagatgaag ccccgatgtt
     6961 atatatcggc gaaaaggttg gtagtggtca aggccatcaa attgatattg ctattgatcc
     7021 cctagaaaat accaatgcta cggcaaattt tgccaacaat gcgatttcgg ttttagccgc
     7081 ctccgaaaaa ggaggcctgt ttcacgcccc tgatatttat atgaataagt tgattgtcgg
     7141 tgaacaggcg gccggtaaag ttgatcttga tgctaaaccg gcggagaatt tgaaaatttt
     7201 agcggaggct cttaaaaggg agatacggga tttagtggtg gttattcttg atcgagagcg
     7261 gaatgaagag ttaattgctc gagttcgaga agccggcgct cgggttaaat tggtggctga
     7321 tggtgattta atgcccggcg tagcggtttg tcttcgcggc tccggtgttc atgccgttat
     7381 ggggattggg gctgctccgg agggagtaat tagtgccgcc gctgttagtt gtcttaaggg
     7441 tgaatttttg ggtcgttttt ggcctaaaaa ttcacaggag gagaagaaat taatccaaat
     7501 gggtggtaac ttggctaaaa tctataatca agctgaatta gcatcaggag agaatttggt
     7561 tttctgtgct accggaatta ctggtgggga tttacttgaa ggagtgcgat ttttcggcgg
     7621 cggtgctaaa actcagactt tagttttagc caagaaaaca ggtaaagttc gttttattac
     7681 caccgtccat gttttagata gaaatgagat tgaatttcga ctttaatgaa aaacgcatta
     7741 agaacttatt attcattttt atggcgttat aagacttatt ttctactttt ctttttttgt
     7801 ttaattttaa atgcgattgc gtggagttta agcccttatt tttacaaact ttttctcgac
     7861 aactttgctt ctactgattt gtcgttagtg atcaaaattt taatcatttt cgccttagtt
     7921 aggataattg ccgttttaag tgaggttgga gccggtatcg ccggcgataa aatgttagta
     7981 cctgcttccc gtgatgcccg actgaagttt tttcagcgga ttaatgaatt ggattttgct
     8041 tatcatcttt ctcaaaaaac tggttcttta attagtacag ttaaacgggg tgatggtgct
     8101 tttattaatc ttcattttat gcttcattgg caggttggca gtattttaat taattttgta
     8161 gtgatgcttt tctttttaat caggttgaat ttttggttgc tgattgccgt tttatttgct
     8221 tttattctag gtttgataac ggctcggcaa ttaatccggc agaatactca agctagaagg
     8281 gagtttattc agtcagatga ccgggtcatg gatgttatta ctgataattt aatgaattat
     8341 gaaacagtta aattgtttgc caaagaaaaa tgggaattat tccgtttggg agaaattcat
     8401 tacgattgga tgaaaaaatt ccgccgttat aactggagtt ttagaaaaat tgatataact
     8461 acaggtatga ttaataatct cggcctttta atagtccttg ttttaggttt aattcagctt
     8521 cgctctcaaa ttatttccct gggcgattat atgatggtgc ttggttttat gaccagcttt
     8581 tatccgcgtt tttttgatct tatttacagt gctagacata ttattcaatc cggagtggat
     8641 atcgaaaaat attttcaaat tttcgatgaa ccagtgttag ttcccgatcc aatcaaaccg
     8701 gttaaaagag actttattat aggcgaaatc gagtttaggc gagttacttt tacctatccc
     8761 gataataaaa gaccagctct gaaagatttt tctcttcata ttcaccctgg ccaagcaatt
     8821 gccttggtgg gaaaatcagg ggtaggaaaa acaactttag gtcgattaat tctccgtttt
     8881 tatgatgttc aaaaaggcca aattttaatt gacggtatca atattaaaag gtttagcaaa
     8941 gatcaattac ggtcttttat tggtgttgtg cctcaggagc cgatcttgtt taatgatact
     9001 atcggttaca atattgctta tggccgagct aaagctagtc aggaagaaat tatagctgcg
     9061 gctaaaatgg ccaatctaga cgagtttatt aatggtcttc ctcagggtta tgatacttta
     9121 gttggagaaa gaggagtgaa attatccggc ggccaaagac agcgtttggc tattgctagg
     9181 atgattttga gcaatcctag cattgtcatc tttgatgaag ctacttccca gttagatact
     9241 gaatcggaaa agaaaattca atcagctttt tggcaagcag tcaaaaataa aacaacgatt
     9301 atcattgccc atcggttatc aactgtgatt aatgctgata aaattgtggt tcttcatcag
     9361 ggacaaattg ctgaaatagg ttctcatcag gaattaatcc aaaaaactaa tagtctgtat
     9421 tcttactttt ggcatcttca gacccaaagt gaaatcttgt agacagtctg tttccatgtt
     9481 aaaacctaat atgaaatttg ttttcgtttc tgggggggtg gtttctggat tagg
//
LOCUS       DAOOIE010000005         9244 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_6567_length_9244_cov_3.418000, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000005 DAOOIE010000000
VERSION     DAOOIE010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 9244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9244
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6567_length_9244_cov_3.418000"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..441
                     /locus_tag="PK131_00365"
     CDS             112..441
                     /locus_tag="PK131_00365"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44625.1"
                     /translation="MNPQTWSSRCAVDGVATIQGFECLFANILQFVVVGAGLVFFVMF
                     IIAGYQLLFASGDPKKAAAASATITHAAIGLIGAICSVLILKLLQQFTGINLTNFVIP
                     GGPTSAL"
     gene            438..1160
                     /locus_tag="PK131_00370"
     CDS             438..1160
                     /locus_tag="PK131_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44626.1"
                     /translation="MKRFLIILILISITFLTFPQPAAAFELADLINFFNKIFAPKYSI
                     NSTTPQQINEYVDNNVGVAKRIMPNSLDNNMKLQYIIDYVNNKAENIDNLGSCPGASG
                     KVDLNQLAFFVLKNYQVPLDQMVINDFLDKNKNLVLQGSNACFNQIWNNFYNVPQGND
                     NEKTDSYQGNQVIREPIPEKSQTGSHLTPKSDKNIIKQDSQEKLDLLHRYLIPDQAMG
                     KNTKERTRLFENFLTPEKEKKE"
     gene            1176..2699
                     /locus_tag="PK131_00375"
     CDS             1176..2699
                     /locus_tag="PK131_00375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44627.1"
                     /translation="MSITAMICRLNRRDQPILKLNSLNKTLLLIALVIFSFIYVKNII
                     AQENPILETQKAIQKGNNQEAWLDNAMGSNLVSLTNLLVGTLPEATYSLDEGEYQQPT
                     SWIPGGAVGTVSQMIASLYNPPASGIEYLAQVKNEFLGKPAYAQSGLGWSGLQPLLPI
                     WRVFRNVIYIVASLFLIILGIMIMLRIRINPQTVITIQNTIPKVIITLILVTFSYAIA
                     GLLIDLMYFVQAAGIYLMFQIKNTDINQSLLAGNNNFWALNQASLSRHFGLIWKMVPS
                     GLLMGLCGLLGAVLTVIGTSTFGPLGLIGLLIPFVLVAIFLLAQVLSLLLGLIKSYFS
                     ILMSIVFAPFIIGAGAFPNSKTGFESWLKQFLANLAVFPAVYLFLILVNVLISMISEP
                     GSTTTLWAPGIISFATPYLSLVIGIAAIMIIAKIPTILPLVLSGSKPSPWGQALTETA
                     QKNIFVTTGRMITPVALHSVSDHLKERIWGTARDETGDERGDETTRKKKPKPPHTTH"
     gene            complement(2702..5050)
                     /locus_tag="PK131_00380"
     CDS             complement(2702..5050)
                     /locus_tag="PK131_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44628.1"
                     /translation="MSEESKDKNLWVNLEKKTGGGNLDSYAKLITQIFNENLSGEYRD
                     EIGAKLKDYLKQHNQEEQFSKIVLRALVIKAGQDEKYMKPLMSGSLKDLATIMPASLQ
                     NEDFLEEVRSGYLNYQPLLDEINKFDTILSSTEGGQPNDKDIEEYKERLSALLNKPNV
                     TSEQKDQINWVLSNPQLTPAKAAEKGDQEPTPKPLSEEVNNLLVDYNKTIKALESYPY
                     LAAIELNRFERSLFNLLKDYPSDSDEAKQIKETLFDIHNRLEGMSGERSYEITDELLA
                     DRTALFEVTEELLDKLEKTGKSPDAITSEVPRYSGPLNEIFCKASEENNDIRGMIQVR
                     LALIECRKMQEEVKGNLEELGRRCNDLRRDGLVRVYTNDILMGVDGTRQMNGRRVEFL
                     LKTMTNQTLAKLHNGDKEAQKGYLVGIGYDWMQDCILNYFERKGGVEIGKYKEGAVSK
                     EQSSMGVVEEAFRYKHRGKANLEQLVAEQKKEGGILHKGKPVIGEHGEQPARYMNYFI
                     DDNPERKALVLDFLINQMKEYGLDDQHAKKTMTLAEDVIWALGIRSKANWAFDGGDEY
                     SEMIYFDDLIYDNSLKGKQVGSKVIRGLIKSIVPDFIQYSQKEFDPDTFISWQDIDYS
                     KIRGKRDFDAYFGMVVPRKASVVRGLLLDPPTDEKKVLTPEFFNGINDAFLKCEPDNM
                     NKDMRAIFVADLLERVLQYPDTTWTKKEINQLKTLVTKNTVAVDEDVQSENINPLRTH
                     FITMEQWKWAYEVVGVGELLKKNRQWRNRLALAESLEKYFRH"
     gene            complement(5043..6989)
                     /gene="ftsH"
                     /locus_tag="PK131_00385"
     CDS             complement(5043..6989)
                     /gene="ftsH"
                     /locus_tag="PK131_00385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPD44629.1"
                     /translation="MPFLKKLTPKSKSAAKKLEPKKENKKSTDELLRNLPPGVKVTKI
                     EIGPKQILRALVYFILIYWLISTLMQIFSPGVVEKVPLSQTIEAIKNNRVAEVTVMDN
                     EIMAKLKDEDKIMVSSKEPSVSMAEILQKEDINLSQVKFNVENRTGWKALGEIVSLLF
                     TVAVPILFILWFFRKQGGGTGGLFNFGKSTAKLFVKGKQSVTFKDVAGVEEAKKDLEE
                     VVDFLKNPEKYRKLGARAPKGVLLVGPSGVGKTLLAKATAGEADVPFYAMAGSEFMEM
                     LVGVGASRVRDLFATAKKAGKAIIFIDEIDAIGRMRGGIDGGHGEREQTLNQILVEMD
                     GFEPTTAVVVLAATNRGDLLDPALMRPGRFDRRVVLEMPDLEARKEIIAIHARGKPFT
                     KEINWEAIAKRIVGFSGADIENMLNEAAIAVARTNRHEITMEDIEEAALKAKLGSEKR
                     RSQTKEDKSMTAYHEAGHAIVNYFEKLDPVTRISIISRGMALGFTLIPPEKDIIHQTK
                     SRLLRQMAMAMGGRAAEEIQFGDISTGAASDITQATNIARNMVVEWGMSNLGPINLGP
                     QIDMEEFNRAYFEPTRISDQMMAKVDDEVKRLVDTAYQKALIILKKKKSLMDELAAIL
                     VEKETIGQEEFEELMKKGKIKKDE"
     gene            complement(7033..7332)
                     /locus_tag="PK131_00390"
     CDS             complement(7033..7332)
                     /locus_tag="PK131_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HPD44630.1"
                     /translation="MEKKNFIRKKENFVCQHCGKRVIGNGYTDHCPYCLWGRHLDEEI
                     PGDRASPCRGLMKPEYAVYQKGEIVIYYKCLSCGHKFQVKAGRNDDYGKITEIMR"
     gene            complement(7340..>9244)
                     /locus_tag="PK131_00395"
     CDS             complement(7340..>9244)
                     /locus_tag="PK131_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HPD44631.1"
                     /translation="PVAIKPDSYSLISPTGELLKVKQVKKKYWPRILIILAVISMLFW
                     PVKWLSNLVLLKQTINRVEELIKIGQSTEAIKIINQRLTVIRKADEQINNWSLNRVSQ
                     IRHLQEIFRLGQSVLVIEEKAAEVGERMAKINQGLFGEAEVDWSKELSKLPRLLDELE
                     IEIGLFEARFTGSSSWLPPKFKEAISYRLKDLNQNRDKIAAVKQLLPIIPTIIGIDGQ
                     RREYLVLLQNESELRPTGGFIGSFGILSFQNGKLLNFEVKDVYEADGQLEGHVEPPAE
                     IRDYLGEASWFLRDANWQANFTGAAKDLEWFLEKEIGRKVDGVISVNLAVARAVLEVV
                     GEITIPDFKEKINKDNLYDQAEYYAEAKFFPGSSQKASFLSALGEQLFESIKTLDTTK
                     RGQLLIALIDKLDEKEVQLAFDDSSAAQGVAALGWDGSIKEGTCASGRCLADYTFINE
                     SNFGVNKVNYFIRRRVEKNIAIGSNLLSRDLKINYENMAKTINYPAGDYKNYLRVYLP
                     REINLETVVIQDGAGNQIEVVDGEKLKLDEKYGKKEIGFLVIVPVSQKRIVRIKYNSP
                     LALTDQFSYLDYLQRQSGSGETEWIESVAYPAEYQPIQVEPVATTIGNKVLFQVKLDR
                     DLKIGVEISR"
BASE COUNT         2611 a         2155 c         1439 g         3039 t
ORIGIN      
        1 gcctgggcta ttgtggcttt gatcaatacc atcttcggta ttaacctcct caatttcacc
       61 atccgaccaa tatccagcac cagtggagtc taaccgtttc taccatttat tatgaatcca
      121 caaacgtggt cctcccgttg tgcggttgat ggggtggcaa ctattcaagg ttttgaatgt
      181 ctttttgcca atattctcca gtttgttgtg gtaggggccg ggttagtttt cttcgttatg
      241 tttattatcg ccggctatca gcttcttttt gccagcggtg accctaaaaa ggccgccgcg
      301 gcttcggcca ctattaccca tgcggctatt ggtttaatcg gagcgatttg ctctgttctt
      361 attcttaaac ttctccagca attcaccggc attaatctta ctaattttgt tattcccggc
      421 ggacctactt ctgctctatg aaaaggttct taatcatctt gattttaata tcgataactt
      481 ttttaacatt tcctcaaccg gcggcggctt tcgaattagc tgacctgata aattttttca
      541 ataagatttt tgcccctaaa tacagcatta attcaacaac tcctcaacaa atcaatgaat
      601 atgttgataa taacgtcgga gtagccaaac gaatcatgcc taattcttta gataacaaca
      661 tgaaacttca atacattatc gactacgtaa ataataaagc ggaaaatata gataatttag
      721 gtagctgtcc cggagcttct ggtaaggtcg atcttaatca attggccttt tttgtcctta
      781 aaaactatca ggttccttta gaccaaatgg tcattaatga ctttttagac aaaaataaaa
      841 atttagtcct ccaggggagt aacgcttgtt tcaatcaaat ctggaacaac ttttacaatg
      901 ttccccaggg aaacgataat gaaaaaactg atagttacca aggtaatcaa gtaatcagag
      961 aaccaattcc tgaaaaaagt caaactggta gtcatcttac tcccaaaagt gataaaaaca
     1021 ttattaaaca agattcccaa gaaaaacttg atctgctcca ccgctatctt attcctgatc
     1081 aagctatggg taaaaacacc aaagaaagaa ctcgtctttt tgaaaatttt ctaactcctg
     1141 aaaaagaaaa aaaagaatag tgctacaata aaataatgtc tattactgcc atgatttgcc
     1201 ggcttaatcg tcgggatcaa ccaattctca aactcaactc tttaaataaa actcttttgt
     1261 taattgccct agttattttt tcctttattt acgttaaaaa tataatcgct caggaaaacc
     1321 ccattctcga aacccaaaaa gccattcaaa agggcaataa ccaagaagcc tggctggata
     1381 acgccatggg gagtaattta gtttctttaa caaatctttt agtaggaact ttgcccgaag
     1441 caacttactc tttagacgag ggagaatatc aacaaccgac ctcttggatc cccgggggag
     1501 ctgtcggcac tgtcagtcag atgattgctt cattatacaa tccaccggca tcgggtattg
     1561 aatatttagc tcaggtaaaa aatgaatttc ttggtaaacc ggcttatgct caatccggtc
     1621 ttggctggag cggccttcaa ccacttctcc ctatttggcg ggtttttcga aatgtaattt
     1681 atattgttgc ctctctattt ctaattattt tgggaattat gatcatgctt cggatcagaa
     1741 ttaaccccca aacagtcatt actattcaaa atactattcc caaagtgatt atcactttaa
     1801 ttttagtcac cttttcttat gccatcgccg gtcttttgat tgatttaatg tattttgtcc
     1861 aagcagccgg tatttatctt atgttccaaa taaaaaacac tgacattaat caatctctct
     1921 tagccggtaa taataatttc tgggccctta atcaagcttc tttgagtcgc cactttggtt
     1981 taatttggaa aatggttcct tcagggttac tgatgggtct atgtggcctt ttaggagcag
     2041 tcttaactgt aatagggaca tctacctttg gccccttggg tttaattggc cttttaatcc
     2101 cctttgttct agtcgccatt tttttactgg ctcaggtact ttcattgctt ctaggtttga
     2161 ttaaaagtta cttctccatt ttaatgtcta ttgtttttgc cccttttatt atcggagctg
     2221 gggctttccc taattctaaa accggctttg aatcatggtt aaagcagttc ttagctaatt
     2281 tagcggtttt tcccgccgtt tatcttttcc ttattttagt taacgtcttg atttccatga
     2341 ttagtgaacc aggttcaacc actactctct gggcgccagg aattattagc ttcgccactc
     2401 cctatttgtc tttagtgatt ggtattgccg ccatcatgat catcgctaaa atacctacca
     2461 ttttaccctt agttctcagc ggcagcaaac cttctccttg gggtcaagcc ttaactgaaa
     2521 ctgctcagaa aaacattttt gttactactg gccgaatgat aactcccgta gctctccata
     2581 gtgttagtga ccacctaaaa gaaagaatat gggggactgc tagagatgaa acaggagatg
     2641 aaagaggaga tgaaacaaca agaaagaaaa aaccaaaacc accccatact actcattaaa
     2701 attaatgccg gaaatatttt tcaagacttt cagccaatgc taatcggtta cgccattgtc
     2761 tgtttttttt caataattcc ccgaccccta ctacttcata agcccacttc cattgctcca
     2821 tagttataaa atgggtcctt aagggattaa tgttttctga ttggacatcc tcatcaacag
     2881 cgaccgtatt cttagtcacc agagttttta attggtttat ttcctttttg gtccaggtag
     2941 tatcaggata ttgtaaaacc ctctcgagca aatcggccac gaaaatcgcc cgcatgtcct
     3001 tattcatatt atcgggttcg catttcaaaa aggcatcgtt aattccatta aaaaactccg
     3061 gggttaaaac ttttttctca tcggtaggtg gatctaataa taatccccta accacgctgg
     3121 cctttcgagg aacaaccata ccaaaatagg catcaaaatc ccttttacct ctaatcttgc
     3181 tgtaatcaat atcttgccaa ctaataaaag tatccggatc aaattctttc tgagaatatt
     3241 gaataaaatc aggtacaata cttttaataa gccccctaat taccttggat ccgacttgtt
     3301 taccctttaa cgaattgtca taaatcaaat cgtcaaagta aatcatttcg gaatactcat
     3361 caccgccatc aaaagcccaa ttagccttgg atcttattcc taaagcccaa ataacatcct
     3421 cagctaaagt cattgtcttc ttggcatgtt gatcatccaa gccgtattct ttcatttgat
     3481 tgattaaaaa atctaaaact aaagcctttc tttccggatt atcatcaata aaataattca
     3541 tatatctggc tggttgttca ccatgttcac caataactgg tttaccttta tgtaaaattc
     3601 ctccttcttt tttctgctca gccaccaact gttctaaatt agcttttcct cgatgcttat
     3661 aacgaaaggc ctcctctact acccccatcg agctctgctc tttggaaaca gctccctctt
     3721 tatatttccc gatctccact cctccctttc tttcaaaata atttaaaatg caatcctgca
     3781 tccaatcgta gcctatccct actaaataac ccttctgggc ttccttatca ccattgtgaa
     3841 gcttggctag agtttggtta gtcatggttt taagcaaaaa ttccacccgt cggccgttca
     3901 tctgtcgggt gccgtcaacc cccattaata tatcgttagt gtaaaccctc actaaaccat
     3961 cccgtcttaa atcgttacat cgccgaccta attcttctag attacctttt acttcctctt
     4021 gcatcttacg acattcaatt aaagccaacc taacctgaat catacctcgg atatcattat
     4081 tttcttctga agctttacaa aaaatttcat taaggggacc ggaataccta ggaacttcag
     4141 aggtgatggc gtccggactt ttacccgttt tttctaattt atctaataat tcctcagtaa
     4201 cttcaaaaag agctgttctg tcagccaata attcatcagt aatttcatag ctcctttcgc
     4261 cactcatgcc ttcgaggcgg ttatgaatat caaataaagt ctctttaatt tgtttagcct
     4321 catcactatc cgaggggtaa tcttttaaaa gattaaataa agatctttca aaacggttta
     4381 attctattgc ggctaggtaa ggatagcttt ctaaagcttt gattgtttta ttataatcaa
     4441 ctaacaaatt attaacttct tcgcttaatg gttttggggt tggctcttga tcgccttttt
     4501 ctgccgcttt cgcaggagtc agctgaggat tactcaatac ccaattaatt tgatcttttt
     4561 gttcagaggt aacattaggt ttatttaata aagcggataa tctttcctta tactcctcaa
     4621 tatctttgtc atttggctgt cctccttcag tggatgacaa aatagtgtca aatttattta
     4681 tttcatctaa taaaggttgg taatttaaat aaccgcttct aacttcttcc aagaagtcct
     4741 cgttttgtaa agacgccggc ataatggttg ccaaatcctt caaacttccc gacattaaag
     4801 gtttcatata cttctcatcc tggcccgcct taatcaccaa tgctctcaac acaattttac
     4861 tgaattgctc ctcttggtta tgctgtttca aataatcctt taacttagct cctatctcat
     4921 cccgatactc tccgcttaag ttttcattaa agatctgggt gattaatttg gcatatgagt
     4981 ccaaattacc accgccggtc tttttttcca aattaaccca taggttctta tctttacttt
     5041 cttcactcat ccttttttat tttacctttt ttcattaatt cttcaaactc ttcctggccg
     5101 atagtctctt tttcaaccaa aatagccgct aattcatcca tcagactttt tttcttcttt
     5161 aaaataatca aggccttttg gtaagccgtg tcaaccaacc gcttaacttc atcatcaact
     5221 ttagccatca tttggtcaga aattctagta ggttcaaaat aagcccggtt aaattcctcc
     5281 atatcaatct ggggtcctaa atttatcggt cctaaatttg acatacccca ttccaccacc
     5341 atatttctgg caatattagt cgcctgggtg atatcactgg cggcgccggt cgaaatatca
     5401 ccaaactgaa tttcctcggc ggctcggcca cccatcgcca tcgccatttg tctcaacaat
     5461 cttgatttag tttgatgaat aatatctttt tcggggggta tcagagtaaa acccaaggcc
     5521 atcccccgcg agataataga aatcctagtt accggatcta atttttcaaa ataattaacg
     5581 atagcatggc cggcttcatg gtaagccgtc attgatttgt cttctttagt ctggcttcgt
     5641 ctcttctctg accccaattt agccttcagg gctgcctctt caatatcttc catagtaatt
     5701 tcatgtctat tagttcgggc gacagcaatc gcggcttcat taagcatatt ttcaatatcg
     5761 gctccagaaa aaccaactat tcttttggct atcgcttccc aattaatttc tttagtaaaa
     5821 ggcttgcctc gggcatgaat ggcaataatt tctttcctag cctctaaatc gggcatttct
     5881 aaaaccaccc gtcggtcaaa tcgacccggc ctcattaggg ccggatcaag caggtcgcct
     5941 cgattagtag ccgctaaaac tactacggct gtagtaggtt caaagccgtc catttcgacc
     6001 aaaatttgat tcaaagtttg ttctctctcg ccatgaccac catcaattcc ccctctcatt
     6061 cggccaatgg catcaatttc gtcaataaaa ataatcgcct tgccggcttt cttagccgtg
     6121 gcaaacaaat ctcgaacccg agaggcaccc acaccaacta acatctccat aaattctgat
     6181 ccggccatag cataaaaggg aacatcagct tcgccagcgg tagctttagc taaaagggtc
     6241 ttaccgactc cgctgggacc aaccaacaaa actcccttcg gcgctcttgc acctaacttt
     6301 ctgtatttct caggattttt caaaaaatca actacctcct ctaaatcttt tttggcttct
     6361 tcaaccccag ctacatcttt aaaagtaacg ctttgtttgc ccttaacaaa taatttggca
     6421 gttgacttac cgaaattgaa taaaccgccg gtgccaccgc cttgttttct gaaaaaccag
     6481 agaataaata gaatcggcac cgccacggta aataaaagac tgactatttc ccctaaagct
     6541 ttccagccgg ttcgattttc gacattaaat ttaacctgac tcaaattaat gtcttctttc
     6601 tgtaaaatct cggccattga tactgatggt tctttcgaag aaaccataat tttatcttca
     6661 tccttaagtt tagccataat ttcgttatcc attaccgtta cttcagccac ccggttattt
     6721 ttaatcgcct caatagtttg ggataacggc accttttcta ccactcccgg agaaaagatt
     6781 tgcattaaag ttgaaattag ccaataaatc aaaatgaagt aaaccaaagc tcgcaaaatc
     6841 tgcttaggtc cgatttcaat tttggtcact tttactcctg gcggcagatt acgcaataat
     6901 tcgtcagtag atttcttgtt ttctttttta ggttctaatt ttttcgctgc cgatttactt
     6961 ttgggagtta atttctttaa aaagggcata ttggttatta taacctagat ttaattacta
     7021 attattaatt aattaccgca taatctccgt aattttaccg taatcatcat ttctaccagc
     7081 tttcacctga aacttatgac cacaagacag gcatttataa taaatcacta tctccccttt
     7141 ttggtaaact gcatattccg gcttcattaa tccccggcac gggctagccc gatcgccagg
     7201 aatttcctca tccaaatgtc ttccccaaag acaataaggg cagtggtcgg tgtagccatt
     7261 acctattact cttttaccac aatgctggca aacaaaattc tccttttttc taataaaatt
     7321 ttttttctcc attaatcaat taccggctaa tttcaacacc aatctttaaa tctctatcca
     7381 acttaacctg aaacagaact ttattaccga tggttgttgc taccggctca acctgaattg
     7441 gctgatattc ggccggataa gcgactgact ctatccattc agtttcacca ctgcctgact
     7501 gccgctgtaa ataatccaaa taagaaaact ggtcggttaa ggctagagga ctattatatt
     7561 taatccggac aattcttttt tgagacacgg ggacaatcac taaaaagccg atttcttttt
     7621 tgccatattt ttcatctagt tttaattttt ctccatcaac aacctcaatt tggttgccag
     7681 ctccatcctg aatcaccacc gtctctaaat ttatttctcg aggtaaataa acccgcaagt
     7741 aattcttgta atctccagcc ggataattaa ttgttttagc catattttca taattaattt
     7801 tcaaatccct tgatagtaaa ttactcccaa tagcaatatt tttttccact cttcttctta
     7861 taaaataatt gactttatta acgccaaaat tagattcatt aataaaggta taatcggcta
     7921 aacaacgacc ggaagcacaa gttccttctt tgatcgatcc gtcccaacca agggcagcga
     7981 ctccttgagc cgctgatgaa tcgtcaaaag ctaattgaac ctccttttca tctaatttat
     8041 ctatcaaggc aattaataac tgaccccttt tggtagtgtc taaagttttt atggattcaa
     8101 ataattgttc tcccagggct gatagaaaac tagccttttg actggaacca ggaaaaaatt
     8161 tagcttcagc ataatattca gcctgatcat aaaggttgtc tttattaatt ttttccttaa
     8221 aatcgggaat ggttatctca ccaactactt ctaaaactgc tcgggcgacg gctaaattaa
     8281 cactgatcac cccatcgact ttgcgaccga tttctttttc caaaaaccat tccaaatctt
     8341 tagccgcccc ggtaaaatta gcctgccaat tagcgtcccg caaaaaccag ctggcctcac
     8401 cgagataatc cctgatttct gctggtggtt ccacatgacc ttctaactga ccatcggcct
     8461 cataaacatc tttaacctca aaattcagca gtttgccatt ttgaaaactt aaaataccaa
     8521 aagaaccaat aaagccgccg gtaggtctta attcactttc attctgaagt agaaccaaat
     8581 attcccgccg ttggccatca ataccaataa ttgttggaat tataggtagt aattgtttta
     8641 ccgccgctat tttatcccga ttttgattta aatcttttaa tcgataacta attgcctctt
     8701 taaattttgg cggcaaccag gaactgctgc cggtaaatct ggcctcaaaa agaccaattt
     8761 cgatttcaag ttcatctaat aaacgtggca gtttgcttaa ctcttttgac caatccactt
     8821 ctgcttctcc aaataagccc tgattgatct tggccatcct ttctcccact tcagccgcct
     8881 tctcttcaat aaccaaaacc gattgaccta gccggaaaat ctcctgtaaa tgtctaattt
     8941 ggcttacccg attcaatgac cagttgttaa tctgttcgtc tgccttccga atcactgtca
     9001 ggcgctgatt aattatttta atcgcctcag ttgattgacc aatcttaatc aactcttcca
     9061 ctcgattaat tgtctgtttt aaaagcacca agttacttag ccacttaact ggccaaaata
     9121 acatcgaaat tactgctaaa ataattaaaa ttctgggcca atattttttt ttcacctgtt
     9181 tcaccttaag caattcgccg gttggggaaa ttagagagta actatccggt ttaatcgcca
     9241 ctgg
//
LOCUS       DAOOIE010000006         8845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_6911_length_8845_cov_3.665415, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000006 DAOOIE010000000
VERSION     DAOOIE010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 8845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8845
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6911_length_8845_cov_3.665415"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..838)
                     /locus_tag="PK131_00400"
     CDS             complement(104..838)
                     /locus_tag="PK131_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44632.1"
                     /translation="MGLKDDIKKTIAYAKTYGCVLNKKQLYERLIGKNNYELKITSYE
                     FKHLKLRKAQQLAERVGKMFPNVLFIGVTGSVAAEFPKRNDDIDLFIIVKNNTLWLTR
                     FWLRIFIWQQKIPHRRYGRKEKADEFCFNIWLDESALLLPPQKQNLNNAVDLILMKKI
                     YDKNNTYIKFIQANSWAKKYVTTPYYHLIRNSKSKIQNDLPKILNFKLKILNLLYFLP
                     QYLYSRLKTKPQNVDLHRAFWGGSRR"
     gene            864..2363
                     /locus_tag="PK131_00405"
     CDS             864..2363
                     /locus_tag="PK131_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44633.1"
                     /translation="MNKKTRWYRFLEMLPGLIAWFFILFPLWGAFVIPKLVAYFALAF
                     ATYWLWQSFKTATLAIIGYFKIKAAKNTNWLEKFNQDFRARWLPYRDIHHVIIISSYK
                     EPNDVIEMAVGSLAAQVDIDLKKLHVILAQEARAGKENNQKTIDYFSTKYKGTFGHLI
                     FTEHPSNLPGETAGKHTNEAWAAKYFKKKYIDSGRYQLKNLTLTSCDVDTLFHPKYFS
                     CLTYNFARNHQRYYRFWQAPIIWHRNINQVPALIRIVGIIGNVGHIAGVQELDGLFFN
                     YSCYSSSYYLIDSAGYWDPDVISEDWHIFLQTFFANQGRVAVEPIFLPAVVDAPSGET
                     TWIALRNRYKQCVRQAWGCTDIPYALEQARLHREIPFITRYLRVFKIMESYFIWASNW
                     FLLTLGSSLPVLLNPHFFATTLGFNLPRIVRLILTICLIPLICLIIVDWQLRPADLKG
                     GWRTVLKNLIQWPFLPVATFFLSVLPGLESQTRLMLGGRLEYWTTPKKA"
     gene            2387..3742
                     /locus_tag="PK131_00410"
     CDS             2387..3742
                     /locus_tag="PK131_00410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPD44634.1"
                     /translation="MMFWLTNGLVFFLLRLPSLFEPYWYGDEGIYLTLGQALRRGLTL
                     YSQIYDNKPPLLYYLAALGRTVFGFRLLLFLVMIPTVIYFYLLARKFFNHRLAAVSTF
                     IFIIFTSIPLIEGNVANAEIFMLLPTILAVYQWLNKKYFWSGVFFGLAFTLKAPVVIE
                     AFFIFICLILTQPAGQRRNILFYLIGCSWPIILWTIYFFFKGAVKPFITAAIFQNFGY
                     LSSWQTGSINHSPLNGGLTGRLLILLLVWGGLYWLLKKKLISSKLVFLLGWLSACIFG
                     SLLSGRPYPHYLIQILPPLFLLIGFWPYFILALSIIICLIFRYKFYFYPTIPYYRNFY
                     FNHNNPSYYGEKVATTYKIAEYLKNNTNRAEPIFIWGDEPYIYALADRLPVGRYTAAY
                     HIIDLNAYEETIMALQQRRPKFIVYFSMENRDFPQLDKFLNQNYLPLDQIGPALIFQL
                     K"
     gene            3743..4111
                     /locus_tag="PK131_00415"
     CDS             3743..4111
                     /locus_tag="PK131_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44635.1"
                     /translation="MSLFYSLSLLGKFWQEKMNHFMVRGTIFCILGQIIVLFFTFSNL
                     PTQVPLYYTLPWGESRLAPVANLFLFPLLSVLVLVVDSLMAMYYSRELKLLSRLLMIT
                     SLVFSFFSLIGLLHIIFLVS"
     gene            4116..5174
                     /locus_tag="PK131_00420"
     CDS             4116..5174
                     /locus_tag="PK131_00420"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HPD44636.1"
                     /translation="MISQFIISLIVASLISLMTTPLVRNFFIKRNWVEYPWEKQKKTK
                     NATALAPVPRGGGIPVYLAVVLTIILFLPLDKHLLGIIIACTINLLVGVADDLWDLSP
                     GFRLITNVLAALAIVISGIGIAFLSNPFGGVIDFSLWRYDFTLFGLPHSIWIVSDLLA
                     IIWITWCTNIVGWSTGVDGQLPGLVVIAATFIGILGLRFHNDITQWPVIVLAGALAGA
                     YLGFLPYNIFPQSIMPGYGGKSLAGLLLAVLAILSGAKLATLILLLSVPMVDAIFVII
                     RRLLNHQSIFQSDNRHLHHQLLRLGWKRSQIAVFYWLVSLLFGIISLFLNSTQKFYAF
                     LGIFLLLFSFTWRLSRRI"
     gene            complement(5146..5931)
                     /locus_tag="PK131_00425"
     CDS             complement(5146..5931)
                     /locus_tag="PK131_00425"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPD44637.1"
                     /translation="MLKKAILVIICSLFLTGCSLTPPAKSAIEIHSTPQATVFINGRE
                     AGITPYKNTNLTPQEITVKLAFNDQWVEKKINLKQGLTSVLDWKFDENGNNGGYLLTM
                     EKTGDSNCSLLINSIPARAVISLDNQIIGSTPKKIDRLEQGEKQITISFPNYQNINLF
                     LRTTPGYNLIVTAELIKNIRPTESSQETTPINQQPLVRVRETETGWLNVRDNPSTGGR
                     EITKIKPDEEYQLLEKTQDWLKIELKDGRDGWIAAKYGEIISR"
     gene            complement(5940..6515)
                     /locus_tag="PK131_00430"
     CDS             complement(5940..6515)
                     /locus_tag="PK131_00430"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HPD44638.1"
                     /translation="MLAKTQKIIKRVALLGDSSAKRYQSHWQAAYQTAKILAQNGYII
                     VNGGGPGVMLAATLGAKSAKGIVELSIINPQKKPNNFESTHPGNLALADKVYIANTYQ
                     ERLNRLIDLADAYICFKGGTGTLSEIGLTWGLAKFDYGRHEPLIFYGKFWKTILEQLK
                     TALPLTKDEKTVYAIAQTPKEVLELLKNSQI"
     gene            complement(6487..7086)
                     /locus_tag="PK131_00435"
     CDS             complement(6487..7086)
                     /locus_tag="PK131_00435"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44639.1"
                     /translation="MQIIPTVLEKEFNAAEKTIRQVMTFSPWIQIDVIDGIYTQGKTF
                     ELELLNKAEFPETILWDIHLMVKEPENWLEKCFFVGAARITGQVEMMTDRYRFINKIK
                     SMNIEAGLAFDQNTPATNIPPETNLVLLMGRKAGFIKSEFDPQVLKKINHQFITAIDG
                     GVNFQNIKTIEEAGVDIVYSGRAFRDLINHHAGQNSKNN"
     gene            complement(7077..8108)
                     /locus_tag="PK131_00440"
     CDS             complement(7077..8108)
                     /locus_tag="PK131_00440"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPD44640.1"
                     /translation="MDHKIRIGINGFGRIGTVVLRAILLENYNVEVAAINTKGDFDID
                     SIVHQFKYDSVYGRIPFHLEAAGPKLGAELGRLRIEDTEIPILGESNPDEINWKKYQT
                     EVVLECTGAFLDKKAAGHFKGGAKKVIISAPSKDPKIPVYILGVNESRYQGENLISNG
                     SCTTNCVAPIVKVIDQKIGFQEGILTTIHAYTTNQNIVDGIGKDARRARAGAINIVPT
                     STGAAEAVIAAYPEAMGRFAGTAIRVPVVCGSYSELTFKVIRETSVEEINSLLEEASV
                     SPELVGKLKIGYEPIVSSDIIGNNASCLIDTLMTKVVSQDMVSIGAWYDNEYGYSCRL
                     IEMAIFICR"
     gene            complement(8160..>8845)
                     /gene="pgk"
                     /locus_tag="PK131_00445"
     CDS             complement(8160..>8845)
                     /gene="pgk"
                     /locus_tag="PK131_00445"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPD44641.1"
                     /translation="NLRFWPEEEANNDSFMKNLIDHSRAYINDAFAVAHRRHASIMLH
                     QKMDGYYGFAFIEEVEKISHILENPARPLTIILGGAKEDKLSYLPDLVKIADKILIGG
                     KLPQITNYKLSITNEKINWGKLRRDTLDLSQETINQFIEIINNSKTIIWAGAMGYYEN
                     ENSRWGTQKIAEAVALNPGFKIIAGGETAASVIDLKLKDKIDYICSGGGVMLEFLTKG
                     TLPAWEERS"
BASE COUNT         2336 a         1636 c         1616 g         3257 t
ORIGIN      
        1 aaaagtgcca ttactacggc aataattgta gctatgatat ttctccgatg attatctttt
       61 gatttaattt tcacttttta attataaccc tggtcagaca tcattaccgc ctactgcctc
      121 cccaaaaagc tcgatggaga tcaacatttt ggggtttcgt tttcagccgg ctgtaaaggt
      181 attgaggcag gaaatataat aaatttaaaa tttttaattt aaaatttaaa atttttggta
      241 aatcattttg aattttggat ttcgaatttc gaattaaatg ataatatggt gttgtcacgt
      301 atttttttgc ccaactatta gcctgtataa attttatata ggtattattt ttgtcataaa
      361 tctttttcat taaaattaaa tcaacggcat tattaagatt ttgtttttgg ggaggcaaga
      421 gtaaggctga ttcatctagc caaatattaa agcaaaattc atccgccttt tcttttcgac
      481 catatcgccg gtgaggaatt ttttgctgcc agataaaaat tctaagccaa aacctggtca
      541 accatagagt gttattttta acaatgatga ataaatcgat atcatcattt cttttgggga
      601 actctgctgc taccgagccg gtcaccccaa taaataaaac gttaggaaac atttttccta
      661 ctctttcagc taattgttga gcttttcgta gttttaggtg tttgaattcg taactcgtaa
      721 tttttaattc gtaattattt ttgccgatca atctttcgta taattgtttt ttgttgagga
      781 cgcaaccgta tgttttggcg tatgctattg tcttcttaat atcgtctttt aatcccattg
      841 ctgatataat acactattag ccgatgaata agaaaacccg ttggtatcga tttttagaaa
      901 tgttaccggg cttaatagct tggtttttta ttttgtttcc tttatgggga gctttcgtta
      961 ttcctaaatt agtggcttat tttgctctgg cttttgctac ttattggctt tggcaatctt
     1021 ttaaaacggc taccctggct attattggtt attttaaaat taaagcggcc aaaaatacca
     1081 attggttgga aaaatttaat caggatttcc gggccaggtg gttaccatat cgagatatcc
     1141 accatgtaat tattatttct tcctataaag aacccaatga tgttattgag atggcagttg
     1201 gttctttagc ggcccaagtc gatattgatt taaaaaagct gcatgttatt ttggctcaag
     1261 aagcccgagc tggtaaagaa aataatcaaa agacaattga ttatttttct accaagtata
     1321 aagggacatt cggccatttg atttttaccg aacatccgag taatttgccc ggagaaacag
     1381 ctggtaagca cactaatgaa gcttgggcag ctaaatattt taagaagaaa tacattgata
     1441 gtggtcgtta tcaattaaag aatttaaccc tgacaagttg tgacgtcgat actctttttc
     1501 atcccaaata tttttcctgt cttacttata attttgcccg aaaccatcag cggtattatc
     1561 gtttttggca agccccgatt atttggcatc gcaatatcaa tcaggtgccg gccttaattc
     1621 ggatagtcgg tattattggc aacgtaggtc atattgctgg tgtccaagag ttggatggtt
     1681 tgttttttaa ttattcctgt tattcttcca gttattattt gattgatagt gccggctatt
     1741 gggatccaga tgttatttct gaagattggc atatttttct ccaaaccttt tttgccaatc
     1801 agggacgggt ggcagttgaa ccgatttttt tgccggcagt agttgatgca ccatcgggag
     1861 aaacgacctg gatagcttta agaaatcgtt ataaacaatg tgttcgtcaa gcttggggtt
     1921 gcactgatat cccttatgct ctcgaacagg cccgtcttca tcgagaaatc ccgtttatta
     1981 ctcgatatct tcgtgttttt aagattatgg aatcttattt tatttgggct agcaattggt
     2041 ttttgttaac tttaggttca tccttaccgg ttttactcaa tcctcatttt tttgccacga
     2101 cccttggttt caatttacct cggattgttc gtttaatatt gaccatctgc cttattccct
     2161 taatttgcct gattattgtt gattggcaac ttcgcccggc tgatttaaaa ggaggttgga
     2221 ggactgtttt aaagaatttg attcaatggc catttttacc agtggctacg ttttttcttt
     2281 cagttctgcc tggtctagaa tctcaaaccc gcttgatgct aggtggaaga ttagagtatt
     2341 ggacgacacc taagaaggct tgagcttcaa atgctaaaat aacctaatga tgttttggtt
     2401 aactaatggt ttagtttttt ttcttctacg tctaccgtcc ctctttgaac cctattggta
     2461 cggcgatgag gggatttatt tgactttagg ccaggctctg agacgtggtt taactttata
     2521 ttctcaaatt tatgataaca aaccgcccct tttatactat ttagccgctc tgggccggac
     2581 agttttcggt tttcgtttat tgttgttttt ggtgatgatt cctaccgtga tttattttta
     2641 tcttctggct cgaaaatttt ttaatcaccg tcttgccgct gtaagcactt tcatttttat
     2701 tatctttact tccattcctt taattgaggg caatgtggcc aacgccgaga tttttatgct
     2761 tctaccaact attttggcag tttatcaatg gttgaataaa aaatattttt ggtccggtgt
     2821 attttttggt ttagctttta ccctcaaagc tccagtagtc atcgaagctt tttttatttt
     2881 tatttgtctg attttaaccc agcctgccgg tcaacggcgg aatattttgt tttatttaat
     2941 tggctgcagc tggccaataa ttttatggac aatttatttc tttttcaaag gagcagtcaa
     3001 gccttttata acggcggcta ttttccaaaa ttttggttat ctttcttcat ggcaaaccgg
     3061 ttcaattaac cattctccct taaacggcgg tttaactggt cgattattga ttctcctatt
     3121 ggtatgggga ggactttatt ggcttttaaa gaaaaaatta attagttcca aattggtttt
     3181 tcttcttggc tggctatcag cttgtatttt tggcagtctt ttatccggtc gtccttaccc
     3241 tcattatttg attcagattt taccgcccct tttcctcctt attggttttt ggccttactt
     3301 tattttagct ttatcgatta taatttgtct tatttttcgg tataaatttt atttttatcc
     3361 aactatacct tattatcgta atttttattt taaccataat aatccttcct attatggaga
     3421 aaaggtagcc actacttata aaattgctga atatttaaaa aacaacacaa atagagccga
     3481 gccgattttc atctggggtg atgaacctta tatttatgct ttggcggatc gcttgcctgt
     3541 tggtcgttat actgccgctt atcatattat tgatttgaat gcctatgagg aaactataat
     3601 ggcccttcaa cagagacgac ctaaatttat tgtctatttt tcaatggaga atagagattt
     3661 tccccaactt gataaatttc tcaatcaaaa ttatttaccg ctcgaccaaa tcggtccagc
     3721 ccttattttt cagttaaaat aaatgtctct tttttattcc ttatcacttt tgggtaaatt
     3781 ttggcaagaa aagatgaatc attttatggt tcgagggact attttttgta ttttaggaca
     3841 aattattgtt ctttttttta ccttttctaa tttaccaacc caagttcctt tatattacac
     3901 tttaccttgg ggagaaagtc gtttggctcc cgtagctaat ttatttcttt ttcctctttt
     3961 atcagtcctt gtcttggtcg ttgatagttt gatggccatg tattattccc gggaattaaa
     4021 acttttgtcc agattattaa tgattacttc cttagttttc agtttttttt cattgattgg
     4081 tcttctccat attatttttt tggtttccta ataaaatgat aagccaattt attatttctt
     4141 tgatcgttgc cagcctaatt tcattgatga ctactccttt agttcgtaat ttttttatta
     4201 aacgcaattg ggttgaatat ccatgggaaa aacagaaaaa gactaaaaac gccaccgctt
     4261 tggcaccagt accgcgaggt ggaggtattc cggtctattt agccgtggtt ttgactatca
     4321 ttttattcct tcctttagat aagcatttac ttggtattat tattgcctgt actattaatc
     4381 tcttagttgg cgttgccgat gacctatggg acttaagtcc aggttttcgt ttgataacta
     4441 atgttctggc ggccttggct attgtgatta gtggaattgg aattgctttt ttatctaatc
     4501 ccttcggcgg ggtaattgat ttttcccttt ggcgatacga ttttactctt tttggtttac
     4561 cccactctat ttggattgtt tctgatttat tggccattat ctggattact tggtgtacca
     4621 atattgttgg ttggtcgact ggagtggatg gccagctccc aggtttagtg gtaattgcgg
     4681 ctacatttat tggtattctt ggtttacgtt ttcataacga tattactcaa tggccagtta
     4741 ttgttttagc tggggcttta gctggagcct atcttggttt tcttccttat aatatttttc
     4801 ctcaaagcat tatgcctggt tatggtggta agagtttggc cggtctatta ttagcggttt
     4861 tagctatttt gtccggggct aaattggcta ctttaatttt acttttaagt gtacctatgg
     4921 ttgatgccat ttttgttatc attaggcgat tattaaacca ccaatcaatt tttcaatctg
     4981 acaatcgtca tttacatcac cagctattac gtttaggatg gaaacggtcc cagatcgccg
     5041 ttttttattg gttagtttct ttattgtttg gtataatttc tctttttcta aatagcaccc
     5101 aaaaatttta tgcattttta ggaatttttt tgctcctttt ttcctttacc tggagattat
     5161 ctcgccgtat ttagctgcta tccaaccgtc tcgaccatcc tttaattcta ttttaagcca
     5221 atcctgagtt ttttctaaaa gttgatattc ctcgtctggt tttattttgg taatctctcg
     5281 gccaccagtg gaaggattgt cccttacatt taaccagcca gtttctgttt cccgaacccg
     5341 gacgagtggt tgttgattaa tgggagtagt ttcctgagaa gattcggttg gtcggatatt
     5401 tttaattaat tcagcagtaa ctattaaatt atagcctggt gtagttctta aaaataaatt
     5461 tatgttttga taatttggaa agctaatagt aatttgtttt tccccttgtt caagacggtc
     5521 tattttttta ggagtcgaac caataatttg attgtctaag ctaatcaccg ctcgagccgg
     5581 aatcgaatta attaaaagac tgcagttaga atcaccggtt ttttccattg ttaatagata
     5641 accaccattg ttgccatttt catcgaattt ccagtctaag accgatgtta atccttgttt
     5701 aaggttaatt tttttttcta cccactggtc attaaaggct aacttaacag tgatttcttg
     5761 aggagttaaa ttagtgtttt tgtaaggggt tatgccggct tcccggccat taataaaaac
     5821 ggtagcctga ggagttgaat ggatttcgat cgccgacttg gccggagggg ttaagctaca
     5881 gccggttaaa aataaactgc aaataatgac taaaatcgct tttttaagca tttttataat
     5941 taaatttggg aatttttcag taattcaagg acttctttgg gggtttgagc aatggcataa
     6001 acagtttttt cgtctttagt gagtggcaaa gcagttttta attgttccaa aattgttttc
     6061 cagaattttc cgtaaaaaat tagaggttca tgtcggccgt aatcaaattt agctaatccc
     6121 caggttaaac caatttcaga aagagttccg gttcctcctt taaagcagat ataggcgtca
     6181 gctagatcga ttaatcgatt gagtcgttcc tggtaggtgt tagctatata gactttatca
     6241 gccagagcta aattgccggg atgggtactt tcaaaattat ttggtttttt ttggggatta
     6301 atgatgctta attcaactat acctttggca gatttagccc ctaaagtagc cgccaacatg
     6361 actcctggac caccgccatt gacgataata tagccgtttt gggctaagat tttagctgtt
     6421 tggtaagcag cttgccaatg tgattggtat ctcttggccg atgaatcgcc taaaagggcg
     6481 actctcttaa ttattttttg agttttggcc agcatgatga ttaattaaat ctctgaaagc
     6541 tcgcccgctg tagacaatat ccaccccagc ttcctctatt gttttaatat tctggaagtt
     6601 aacaccgccg tcaatagccg taataaattg atgatttatt ttttttaata cttgaggatc
     6661 aaactcactt ttgataaatc cagcctttct tcccattaat aaaacaagat tagtttcggg
     6721 aggaatatta gtggcgggag tattttgatc aaaagccaaa ccagcctcta tattcatact
     6781 ttttatttta ttaataaaac gataacggtc agtcatcatt tctacttgac cggtaattct
     6841 ggcggcacca acgaaaaagc atttttctaa ccaattttcc ggctccttaa ccattaaatg
     6901 aatatcccac agaattgttt ccggaaattc ggccttatta agcaattcca gttcaaaagt
     6961 tttaccttgg gtataaatgc cgtcaataac gtcaatttga atccaaggtg aaaaggtcat
     7021 tacctgtcga attgtttttt cggcggcgtt gaattctttt tctaaaactg tcgggattat
     7081 ctgcatataa aaatagccat ttcaattaga cgacaggagt aaccatattc gttatcatac
     7141 caagcgccaa ttgaaaccat atcttgggaa actaccttag tcattagggt atcaataaga
     7201 catgaagcat tattaccgat aatatcggat gaaacaattg gttcatagcc aatctttaat
     7261 ttaccgacta attcgggtga aacgctagct tcttctaaaa gggaattaat ttcttcaact
     7321 gaagtttctc taatgacttt aaaagtaagc tcagaataac taccgcaaac tactgggacg
     7381 cggatagctg ttccagcaaa tcttcccatt gcttccggat aagcggcaat aacagcttcg
     7441 gcggcgccag tagaagtggg cacaatatta atcgctccgg ctctggctcg tctggcatct
     7501 tttccgatac catcgacaat attttggttg gtggtatagg catgaatcgt agttaaaatt
     7561 ccttcctgaa aaccaatttt ttgatcaatt accttaacaa taggggccac acagttggta
     7621 gtacaagaac cgttggaaat aagattttcc ccttgataac gactttcatt aaccccaaga
     7681 atataaaccg gaattttggg atctttagat ggagcggaga taataacttt tttggcccct
     7741 cccttaaaat gaccggcagc ctttttatct agaaaagctc cggtacattc cagaactacc
     7801 tctgtttgat attttttcca attaatttca tccggattag attctccgag aattggtatt
     7861 tccgtgtctt ctattctcaa tctgcctaat tctgctccta acttcggtcc ggcggcttca
     7921 agatggaagg gaatccgacc gtagaccgaa tcgtatttaa attgatggac aatactatcg
     7981 atatcgaaat cacctttagt attaatagcg gcgacctcga cattatagtt ttctaaaaga
     8041 atggctcgga gaacaaccgt gccaatccgg ccgaagccgt taataccgat tctgatttta
     8101 tgatccatat aatcatttta tgctatctga taaaaaaatg aaatctcttt tgctatgatc
     8161 tatgatcttt cttcccaggc cggcaatgtt cccttagtca aaaactctaa catgaccccg
     8221 ccacctgagc aaatataatc aattttgtct tttaatttaa gatcaataac tgaggcagcc
     8281 gtttcgccac cggcaattat tttaaaacca ggattaagag cgaccgcctc agcaattttt
     8341 tgggttcccc aacggctatt ttcgttttca taataaccca tagcaccggc ccaaattatc
     8401 gtttttgagt tattaattat ttcgataaat tggttaattg tttcctggga caggtcaaga
     8461 gtgtctcttc ttagttttcc ccaattgatt ttttcatttg taattgataa tttgtaattt
     8521 gtaatttggg ggagcttgcc gccgatgaga attttatcgg caattttaac cagatccggc
     8581 aaatagctta atttgtcttc tttagcccca ccaagaataa tagttagagg ccttgctgga
     8641 ttttctaaaa tatgagaaat tttttctact tcctcaataa aagcgaagcc gtaataacca
     8701 tccatttttt gatgaagcat aatcgaggca tggcggcgat gggcaacagc aaaggcatcg
     8761 ttgatataag cccgtgagtg gtcaattaaa tttttcatga aactatcgtt attagcttct
     8821 tcttccggcc aaaatcttag atttt
//
LOCUS       DAOOIE010000007         8717 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_7038_length_8717_cov_3.089009, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000007 DAOOIE010000000
VERSION     DAOOIE010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 8717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8717
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7038_length_8717_cov_3.089009"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PK131_00450"
     CDS             complement(<1..420)
                     /locus_tag="PK131_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004099925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPD44642.1"
                     /translation="MPDFVHLHVHTEYSLLDGLTNIKKLVNRVKDLGMKACAITDHGN
                     MYGAIEFYKTCQKANIKPIIGCEVYVSPTTRFDRTIENRRYQHLILLAKNNQGYKNLM
                     KLVSLAHLEGFYYKPRIDWELLQQYCQGLICASACLEG"
     gene            474..980
                     /locus_tag="PK131_00455"
     CDS             474..980
                     /locus_tag="PK131_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44643.1"
                     /translation="MEEKERIPLTAVIKGVPLVNEYFFGKCRNKMIEAVYSLGGNKNE
                     KLADLTGIGDIDEQAKYCELKHPQKWKLAVCWSEKLGRLLVLQVNGEQKDDNEVRRWG
                     IIYRIDEAFDLCNIFEGRMIKSDGMVVFYPTSKKVETNIKLNKKKFNDLTKIFIEADN
                     FKAFLEEP"
     gene            complement(955..1845)
                     /locus_tag="PK131_00460"
     CDS             complement(955..1845)
                     /locus_tag="PK131_00460"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPD44644.1"
                     /translation="MINFYEQVRQNKIRSTIIVVGFITFILVAAYLISYAFNLGSEFL
                     VGAFIFSLIMTLLSYYSGDQIVLNLNQAIPADRDKYFDFYTVVENLSLANQTPMPKIY
                     VIETPAMNAFATGRDPQHAIVCATTGLLTKLNRTQLEGVIAHELSHIKNYDIRLMMIV
                     SVLIGSLSLLTNMISRSMFFRGGRDRDNNSGNIGIILTLIGFLLLIFSPLIAQLIQLA
                     ISRRREFLADASAVKLTRQPSGLIEALQIIGQDPNILTSASTATASLYITNPFKDKLS
                     SLFSTHPPLAARIKALREML"
     gene            complement(1871..2401)
                     /locus_tag="PK131_00465"
     CDS             complement(1871..2401)
                     /locus_tag="PK131_00465"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPD44645.1"
                     /translation="MYILIGILLIVIVYVIAFYNRLKTALVQIGASNQEIGNQLKRQA
                     DLIPNLVESAKGYLKHEKEIFTQLTDARKAINANQIEKSQKLISKALGALQVIVESNP
                     EFKAATVMTDLMAELRDTSDKLMYARRTLIDLTADYNASLVTFPNSILAGLFNFQPQK
                     GLEVTPEEIKKPEIKF"
     gene            complement(2456..3436)
                     /locus_tag="PK131_00470"
     CDS             complement(2456..3436)
                     /locus_tag="PK131_00470"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF026066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HPD44646.1"
                     /translation="MFVNYTNQEVARFLEKIAIAYELKNKNRFKIAAYENAADSIRNY
                     PQNLEEIWQRDKKELDNIPGVGESILKKLDYLFTYHRYPPKVQALFDSFHPAIFVLTE
                     IANIGPQNAKKLVKNLEFPKDKIKIINKLIDYAKKNKIASIPTFGKKSQELFLKNAKE
                     YLGNVDRLPYETANKLATEIINYLKQKFPQAEIVALGSLRRQSPTVGDIDIAVCYPNS
                     SEIIDYFVNYPKSYGTFDKGPKKASIKLKGNIRVDLMVAPRDSWGDLVQHFTGSKEHN
                     IELRKYARKMGLSISEYGIKDLKTGKLYQFTNEKDLYKFLNYPYPEPEKR"
     gene            3502..3578
                     /locus_tag="PK131_00475"
     tRNA            3502..3578
                     /locus_tag="PK131_00475"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3536..3538,aa:Asp,seq:gtc)
     gene            3634..5091
                     /gene="guaB"
                     /locus_tag="PK131_00480"
     CDS             3634..5091
                     /gene="guaB"
                     /locus_tag="PK131_00480"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018131386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPD44647.1"
                     /translation="MVDSKRNLGKALTFDDITLVPRRSEIVPTAVETTVRLTRKLILH
                     IPLISAAMDTVTESKMAIAMARLGGLGIIHKNLTISQQSDEVDKVKRSESGIISDPIT
                     IAPEEPLKTAVLLMEKYHISGIPVIQDKVLVGILTNRDLRFEKNFNQPIAKVMTRKNL
                     ITAPVGTTLRQAEKIFKRYKIEKLPLVDKDFRLKGLITIKDLRKIMEYPQATKDSRGR
                     LMVGAAVGAMEEMERVEALIAAGVDVLVVDSAHGHSKNVIKKIQQIKAKYPQMIIIGG
                     NVVTSQAVKDLAKAGADIIKVGIGPGSICTTRVVAGIGIPQFTAILECAQAARQNKVT
                     IIADGGIKYSGDITKAIAAGAEAVMLGSLLAGTDESPGEKEYYRGKAYKNYRGMGSIG
                     AMRRGSSERYFQEQQSKLVPEGIEGRVVYKGSLSEVVFQLIGGLKSGMGYCGCKNIKE
                     LQTKTKFREITNAGLKESHPHNVQIIKEAPNYYGF"
     gene            5081..5425
                     /locus_tag="PK131_00485"
     CDS             5081..5425
                     /locus_tag="PK131_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44648.1"
                     /translation="MAFNLKETPANIFLWNSFYWLEVKPAIQTLIGRSKKVEVKDKFY
                     FDGTKRKYTFFKLIFPDQKFSEIYFQKNNEVTVRIYDQSGNLLEKGGSGYETKILATI
                     KQYAAEKDKLTP"
     gene            5459..6748
                     /locus_tag="PK131_00490"
     CDS             5459..6748
                     /locus_tag="PK131_00490"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPD44649.1"
                     /translation="MKRFWWGVLIITGVFLGGLLVGRIGLSRKVVQEEVSQNKYVTFL
                     DEVYQIIKENYWNQLTDEQLSNIFILGTEKLTSQSQNVSKKDRENMKKMLEKVLNQYD
                     NDQKRKEFSATLADVVLSNLEPFGRSRLYSQKEEKALSDVVQNKTEEDRYQDLGVTKE
                     AKPEEIAQAYKEQAAKWNPETNKLPEAKEKYEKIQQAYKTLSDPESRKSYDLAGVEPT
                     MDYKLIRPDVFYVHMTKFSPTTFDEMQRVFEKVKGKPGVNSLILDLSDNIGGAIDGLP
                     YFLGPFIGNDQYAYQFLHQGEKQDFKTRTGWLDSLVQYKKVVVLINENTQSTAELMTS
                     VLKKYNVGVVVGRKSKGWGTVERVFPLKTKIDDQETFSVFLVHALSLKEDGLPIEGNG
                     IEPQIYLDNKNWQKELMERFNTSSLVEATSEIISKWR"
     gene            complement(6729..7334)
                     /locus_tag="PK131_00495"
     CDS             complement(6729..7334)
                     /locus_tag="PK131_00495"
                     /inference="COORDINATES: protein motif:HMM:NF012554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPD44650.1"
                     /translation="MNDPKKERTLVLIKIDGIQRGLIGEIIHRYEQTGLKLVALKMLV
                     PSEKLVEKHYTVDPEWRMKNGLKTIANYQAKGLIPPSTDPLVCSGLTLKKLVKYISSG
                     PVIAMVWQGMHAVGVTRKITGGTEPLTSDVGTIRGDFTIDSYQVSDLDQRSTRNLVHA
                     SSSVEEAEKEIDLWFKKEEIINYKLFNESILYETNLNAILK"
     gene            7413..7721
                     /locus_tag="PK131_00500"
     CDS             7413..7721
                     /locus_tag="PK131_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPD44651.1"
                     /translation="MDKIFKALVATSRRKILTLLRDKEMSVTEIVNSLNLSQATISNH
                     LAVLKKAGLVILRSENKSHYYSLNREIFNKISRELSQFADYGIGEIARRKSESKGLDL
                     "
     assembly_gap    7974..7983
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7984..8325
                     /locus_tag="PK131_00505"
     CDS             <7984..8325
                     /locus_tag="PK131_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009362080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPD44652.1"
                     /translation="EYIVEKGDSLWKIALKRYNDGYQWVEIAKANNLKNVGLILPGQK
                     LNLPELAVKEAPKAMIEGNEYVVKKGDCLWNIAIGKYNDGYKWVDIFQANKGLIKNPD
                     YIEIGMKLQLP"
     gene            8341..8411
                     /locus_tag="PK131_00510"
     tRNA            8341..8411
                     /locus_tag="PK131_00510"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8373..8375,aa:Gly,seq:ccc)
BASE COUNT         2863 a         1314 c         1895 g         2635 t
ORIGIN      
        1 tccttcgaga caggcgctag cacaaatcaa tccctgacaa tattgttgaa gtaattccca
       61 atcaattcga ggtttataat aaaaaccttc caaatgagct aaagagacta atttcatcaa
      121 atttttgtaa ccctgattat ttttagccag aagaatcaga tgttgatagc gacgattttc
      181 aatagttcga tcgaaacgag tggtaggaga aacataaacc tcgcaaccga taattggttt
      241 aatgttggcc ttttggcagg ttttatagaa ttcaatagct ccatacatgt tgccgtggtc
      301 ggtaatagca cacgctttca ttcctaaatc tttgacccga ttaactaatt ttttgatatt
      361 agttaaacca tccaaaagag aatattcagt atggacatgg aggtggacaa aatcaggcat
      421 agcctattat aaaataaaca tatcttaagt ggtgttgtat aatcaggatc accatggaag
      481 aaaaagagag aataccgcta acagcagtta tcaaaggtgt gccactggta aatgaatatt
      541 tttttggtaa atgccgcaat aaaatgattg aggctgtcta tagcctgggg ggaaataaga
      601 atgaaaagtt agcggattta accggtattg gggatatcga tgaacaggca aagtattgtg
      661 agctgaaaca ccctcaaaaa tggaaattgg cagtgtgctg gtcagagaaa ttagggcgtt
      721 tgctggtatt acaggtaaat ggagagcaaa aagacgataa tgaagtgaga aggtggggaa
      781 taatatatag gattgatgaa gcatttgatt tgtgtaatat ttttgaaggc cggatgataa
      841 aatctgatgg aatggttgtc ttttatccca ccagtaaaaa ggttgagaca aacattaagt
      901 taaacaaaaa aaaatttaat gatctgacaa aaatttttat tgaggccgat aattttaaag
      961 catttctcga agagccttaa ttcgggctgc caacggcgga tgagtggaaa aaagagatga
     1021 aagtttgtct ttaaaaggat tagtgatata aagagaagcg gttgctgttg aggctgatgt
     1081 taatatattc ggatcttggc cgataatttg gagggcttcg attagaccgg atggctggcg
     1141 ggtgagtttt acggctgaag catcggctaa aaattcacgg cggcgggaaa tagccagttg
     1201 aattaattgg gcgattaaag gtgaaaaaat caacaacaaa aaaccaataa gagttaatat
     1261 tatgccaatg ttgccgctat tattatcacg atcgcggccg ccacgaaaaa acattgatcg
     1321 gctgatcata ttggttaaaa gagagagact accaataaga acagaaacaa tcatcattaa
     1381 ccggatgtca tagtttttaa tgtgggaaag ttcatgggca ataacgccct caagttgggt
     1441 tcggttaagt tttgtcagca atcctgtagt ggcacagaca atggcgtgct ggggatcacg
     1501 gccggtagca aaggcattca tagctggagt ttcaataaca taaattttag gcattggtgt
     1561 ttgattagct aaagaaagat tttctactac cgtatagaag tcaaagtact tgtctcgatc
     1621 ggcaggaata gcttggttaa gattcaaaac aatctgatcg cccgaataat aacttaatag
     1681 agtcattatt aaagaaaaga tgaaagctcc gactaagaat tccgagccaa gattgaaagc
     1741 atagctaata agataagcag ctaccaaaat gaaggtgata aaacccacga ctataatcgt
     1801 tgagcgaatt ttattttgtc ttacctgttc gtaaaagttg atcacctaaa ttaatttaga
     1861 acttaaaatt ttaaaactta atttccggtt ttttaatttc ttctggagta acctctaatc
     1921 ctttttgggg ctggaagtta aaaagtccgg ctaaaatgct gtttggaaaa gtaaccaagg
     1981 aagcgttata atcagcagtc agatcgatca aagtccgacg agcatacatt aatttatcgc
     2041 tggtatcccg taattcagcc ataagatcgg tcataacagt ggcggcttta aattctggat
     2101 tgctttcaac aattacctgt agggccccca acgctttact gattaatttc tgtgattttt
     2161 cgatttgatt ggcattaatg gcttttcggg catcagttag ttgagtgaaa atttcctttt
     2221 catgttttaa ataacccttg gcggattcaa ccaaattggg aattaaatcg gcctggcgtt
     2281 ttaattggtt gccgatttcc tgattggaag cgccgatctg aactagggcg gttttgaggc
     2341 ggttataaaa agcgataacg tagacaataa caataagtaa aataccaatg agaatataca
     2401 tcttagtgat tagtaactag taactagtaa ctagtaatta gtatagcact aatttttatc
     2461 ttttttccgg ttcagggtag ggataattta agaatttata caaatctttt tcgttggtaa
     2521 actgatataa tttacctgtc tttaggtctt taataccgta ttccgaaata cttagcccca
     2581 tttttcgggc atacttacgt aattcaatat tgtgctcttt agaaccagtg aaatgttgga
     2641 ctaaatcacc ccaactgtct ctgggggcaa ccattaaatc aacccggata ttacctttta
     2701 atttaattga agcttttttc ggccccttgt cgaaagtgcc ataactttta ggataattga
     2761 caaaataatc aattatttcg gaagaattgg gatagcaaac ggcaatatcg atatctccca
     2821 ctgttggact ttggcggcgg agtgagccga gagcgacaat ttcggcttga ggaaattttt
     2881 gtttcaagta gttaataatt tccgtcgcta atttgttggc agtttcataa ggcaggcggt
     2941 caacattgcc taaatattct ttagcgtttt ttagaaataa ttcctgtgat tttttaccaa
     3001 aagtgggaat tgaagcgatt ttatttttct tggcataatc aataagtttg ttaattattt
     3061 ttatcttatc tttgggaaat tctaggtttt tgaccaattt tttggcattt tgggggccaa
     3121 tgttggcaat ttcagttaaa acaaaaatag ctggatgaaa actgtcaaaa agggcttgaa
     3181 ctttgggtgg atagcggtga taagtgaaga gataatctaa tttttttaaa attgattccc
     3241 ctactccggg aatattatct aactcttttt tatctcgttg ccaaatttct tccagatttt
     3301 ggggatagtt tctaattgaa tcagcggcat tttcgtaagc agcgatttta aagcgatttt
     3361 tgttttttaa ctcataagca atcgcgattt tttctaaaaa acgggcgact tcctgattgg
     3421 tataattcac aaacatattg tattattttt tgatgttttt tgacaagcaa aaaaaacatt
     3481 gatatattag gtaagttttc ggggtccttg gtgcagcggt ctagcacatt cgcctgtcac
     3541 gcgaaagatc gtgggttcaa atcccatagg gcccgccatg gaagagaaaa agttgtgcta
     3601 caatcaactg tataactggt aaaggttata cttatggttg attcaaaaag aaatttaggt
     3661 aaagcgctga cctttgatga tattaccctg gtgccaagac gatcagaaat agtaccaaca
     3721 gcagtcgaaa ctactgtccg tttgactaga aaacttattt tacatattcc cctaattagt
     3781 gcggctatgg atacggtaac cgaatcaaaa atggccattg ctatggcaag gttagggggt
     3841 ttaggaataa ttcataaaaa tttgactatt agtcagcaat ctgatgaagt tgataaggtc
     3901 aagcgttctg aaagtggaat tatcagtgat ccaattacta ttgcaccaga agagccatta
     3961 aaaacagctg ttttattgat ggagaaatat cacatttcag gaattccggt cattcaagat
     4021 aaggttttag ttggtatttt gactaatcgt gatttacgtt ttgagaaaaa ttttaaccaa
     4081 ccaatagcca aagttatgac tcgaaaaaac ttaattactg cccctgtcgg tactactctt
     4141 cgccaggcag agaaaatttt taaacgatac aaaattgaaa aattaccttt ggttgacaaa
     4201 gattttcggt taaaaggttt gattaccatt aaagatttgc ggaaaattat ggagtatccc
     4261 caagcgacta aggacagtcg aggtcgattg atggttggag cggcggtggg agcaatggaa
     4321 gagatggagc gagttgaggc tttgatcgcc gccggagttg atgttctagt ggtagattct
     4381 gcccatggac acagtaaaaa tgtaattaag aaaattcaac aaattaaagc caaatatcct
     4441 cagatgatca ttattggtgg caatgtagtt acatctcagg cggtgaagga tttagctaaa
     4501 gctggtgccg acatcattaa agtcggtata gggccaggat caatttgtac tactagagta
     4561 gtggcgggta ttggtattcc ccaatttaca gctattttag aatgtgccca ggcagctaga
     4621 caaaacaagg tgactattat tgctgacggt ggcatcaagt attccggcga tattaccaag
     4681 gcaatagcag ctggggcaga ggcggtcatg ttaggtagtc ttttggctgg tactgacgaa
     4741 agtcctgggg aaaaggaata ttaccgggga aaagcttata aaaattaccg aggcatggga
     4801 tctatcggag ctatgcgccg aggtagcagt gaacgatatt ttcaggaaca gcagagtaaa
     4861 ttggtgccgg agggtattga gggtcgagta gtttataaag gatcattaag tgaggttgtt
     4921 tttcagttaa tcggcggctt aaaatcagga atgggttatt gtggttgtaa aaatattaag
     4981 gaattacaga ctaaaacgaa gtttagggaa attaccaatg ctggtttaaa agaaagtcat
     5041 ccccataatg ttcagataat taaagaagcc ccgaattatt atggctttta atttgaagga
     5101 aacaccagct aatatttttt tatggaattc attttattgg ctggaggtta aaccagcgat
     5161 tcaaaccctg atcggtcgga gtaaaaaagt ggaggttaag gataaatttt actttgatgg
     5221 caccaaaaga aaatacacct tctttaaatt aatttttcct gatcagaaat ttagcgagat
     5281 ttattttcag aaaaacaatg aagtaacggt taggatttat gaccaaagtg gtaatttact
     5341 agaaaaaggt ggttcgggtt atgaaactaa aatactagcc actatcaaac agtatgcagc
     5401 cgaaaaggat aagttaaccc cttgaagtaa tagtttattc cccgttatat tagtaaagat
     5461 gaaaagattt tggtggggag ttttaataat aacaggagtc tttttaggag gattattagt
     5521 aggtaggatt ggattgagta gaaaagttgt tcaagaagaa gttagccaaa ataagtatgt
     5581 tactttttta gatgaggttt atcaaataat taaagaaaat tattggaatc aattaactga
     5641 tgaacagtta agtaatattt ttatcttagg aacagaaaaa ttaactagtc agagtcagaa
     5701 tgtgtcaaaa aaagatagag aaaatatgaa gaagatgttg gagaaggttc ttaatcaata
     5761 tgataatgac cagaagagaa aagagttttc ggcaacttta gccgatgttg ttttatctaa
     5821 cttggagcct ttcggccgca gtcggcttta ttcccaaaaa gaagaaaagg ccttatctga
     5881 tgtggttcag aataaaactg aagaagatcg ttatcaagat ttgggagtaa ctaaagaagc
     5941 caagccggaa gaaattgccc aagcttacaa agaacaagcg gccaaatgga atcctgaaac
     6001 taataagtta cctgaagcca aggagaagta tgaaaaaata caacaagctt acaaaacctt
     6061 gtctgaccca gaatctagaa aaagttatga tttagccgga gttgagccaa caatggatta
     6121 taagttaatc agaccagacg tattttatgt tcatatgact aaattttctc caacaacatt
     6181 tgatgaaatg cagagagttt tcgagaaggt gaagggaaag ccgggagtta attcattgat
     6241 tttggattta tcggataata ttggcggggc cattgacggc ctcccctatt ttttgggtcc
     6301 gtttattggt aatgatcaat acgcttatca atttctccat caaggagaaa aacaggattt
     6361 taagacaaga acgggctggt tagatagttt agttcaatat aaaaaggtag tggttttgat
     6421 taatgagaat acccaatcaa cggcagagtt gatgacatca gtcctgaaaa aatacaatgt
     6481 aggggtggtc gtcggcagaa aatccaaggg ctggggaaca gttgagaggg tttttccttt
     6541 aaaaactaaa attgatgatc aagaaacctt ttcggttttt ttggttcacg ccttaagttt
     6601 gaaagaagat ggcctgccca tcgagggtaa tgggattgaa ccacaaattt atttagataa
     6661 taaaaattgg caaaaagaac tcatggaaag atttaataca tcttcgttgg ttgaagcgac
     6721 aagtgagatt atttcaaaat ggcgttaaga ttagtttcat aaagaatact ttcattaaaa
     6781 agtttatagt taatgatttc ttccttttta aaccagagat caatttcttt ttcggcttct
     6841 tcaactgaac tggaagcatg gactaaattt ctagtcgatc gttgatctaa atctgagact
     6901 tgataagaat caatcgtgaa atcaccacga atagtgccga catcggaggt taatggttca
     6961 gtaccaccgg tgattttgcg ggtaactccg acggcatgca ttccctgcca taccatagca
     7021 attaccggac cggaagaaat atatttaaca agttttttaa gagttaaacc ggaacaaact
     7081 aaaggatcag ttgaaggagg gattaatccc ttggcctgat agttagcaat tgtttttaat
     7141 ccgtttttca tccgccattc cgggtcaacg gtatagtgtt tttcaactaa tttttcactt
     7201 gggacaagca ttttaagggc tactaatttt agcccggttt gttcgtagcg gtgaataatt
     7261 tccccgatta aaccccgctg gataccatca attttaataa ggaccaaggt tctttccttt
     7321 ttaggatcat tcatatttct tattttgcct tagcaaaaag gaaaatgcaa ggggcgagtt
     7381 gatatattga tgattatcta tataattaag aagtggataa aatatttaaa gccttagtag
     7441 ctaccagtcg aaggaagatt ttaactctgc tgagagataa agaaatgtcg gtgacggaaa
     7501 tagtaaatag tcttaacctt agccaagcca caatttctaa ccacttagca gttttaaaga
     7561 aagcaggttt agtgatattg agaagtgaaa ataaaagtca ttactactca ttaaatcggg
     7621 aaatatttaa taaaattagc cgtgaattaa gccagtttgc tgattatggt attggcgaaa
     7681 ttgccagacg gaaaagtgaa tctaaaggtc ttgacttata attaagtttg aggtaaagtg
     7741 aactaatccg atccccccgg cgacttcttt atcaggttta aattatttct aaaaatgact
     7801 aagaaaaaga gtagactttt taaatcaacc gaagaagcaa ttagtatgtt tctggggttg
     7861 gttatcgttt tagtagtggt aggtttgata attaactttt taaatgggcg gaaaaaagga
     7921 actaatcttc tcgattctga gcataatgtt actttggagg ggggtaaagt tttnnnnnnn
     7981 nnngaatata tagtagaaaa aggtgattct ttgtggaaaa ttgcattaaa acgatataat
     8041 gatggttatc aatgggtgga aattgctaaa gctaataatt taaaaaatgt tggcctgatt
     8101 ttgccaggac agaagctaaa tttgccggaa ttagctgtta aagaagctcc taaggcaatg
     8161 atcgaaggga atgaatatgt agttaaaaag ggagattgtt tatggaatat tgccattggt
     8221 aaatataatg atggttataa atgggtagat attttccaag cgaataaggg tttgattaaa
     8281 aaccccgatt atattgaaat tgggatgaaa ttgcagttgc cgtgatagaa taaatttatt
     8341 gcggctatag tttagcggta aaatatgtcc ttcccaagga taggtcgagg gtccaactcc
     8401 ctctagccgc tctgataaag ggtattaaaa ttggatcagt ctgttgtcga atttcgatgt
     8461 cggcgtaggc aattaaagaa caaggagtgc tattttcttt ggtaattaat tctgtgggta
     8521 aatcatagag atgggcgcaa agtccctcgg gagcctattt gtggtataat atatattatg
     8581 ggtaaagagg ttatccaacc gagtaatgtt agatttattg gtagcgatcc aatgtttctt
     8641 gattctatta agattgattt cgaaacctat aaagcaaagg caaaaatggg caagttgggt
     8701 gagttgccaa agactgc
//
LOCUS       DAOOIE010000008         8612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_7129_length_8612_cov_3.600093, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000008 DAOOIE010000000
VERSION     DAOOIE010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 8612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8612
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7129_length_8612_cov_3.600093"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PK131_00515"
     CDS             <1..288
                     /locus_tag="PK131_00515"
                     /inference="COORDINATES: protein motif:HMM:NF013428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPD44653.1"
                     /translation="IHTPDSSRFWRAETYKERFKQGLEPENFDKEFLRLWYAERGYKG
                     GGQPPQMSRQLMVDLSRRYLTIYEKLTGKKFKAYRYPVEEAIRRACLDIKM"
     gene            complement(337..419)
                     /locus_tag="PK131_00520"
     tRNA            complement(337..419)
                     /locus_tag="PK131_00520"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(384..386),aa:Ser,seq:tga)
     gene            complement(457..530)
                     /locus_tag="PK131_00525"
     tRNA            complement(457..530)
                     /locus_tag="PK131_00525"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(494..496),aa:Thr,seq:tgt)
     gene            575..647
                     /locus_tag="PK131_00530"
     tRNA            575..647
                     /locus_tag="PK131_00530"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:608..610,aa:Thr,seq:ggt)
     gene            855..1079
                     /locus_tag="PK131_00535"
     CDS             855..1079
                     /locus_tag="PK131_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HPD44654.1"
                     /translation="MIKKTTLIKEITDKYPQLAVILVQQYGFHCLGCWAAGEETLAEG
                     ALAHGYSPREIREMIAVLNKSLLSGKGDRK"
     gene            complement(1018..1578)
                     /locus_tag="PK131_00540"
     CDS             complement(1018..1578)
                     /locus_tag="PK131_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPD44655.1"
                     /translation="MANFLNGINSVIALIIAITIHEFSHAYVADHLGDPTPRSAGRLS
                     LNPLAHLDPLGTLMLFLAGFGWGKPVPIDPYNFRHPRRDSALVSVAGPVSNLIFAVLI
                     TLIAKFFYIPLFGILIFINISLAVFNLLPFHPLDGSHIFPDLFPGRSGMIILILLILP
                     LFQGTALISYLLFPIINFCLKLLSFL"
     gene            1597..1812
                     /locus_tag="PK131_00545"
     CDS             1597..1812
                     /locus_tag="PK131_00545"
                     /inference="COORDINATES: protein motif:HMM:NF013026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L28 family ribosomal protein"
                     /protein_id="HPD44656.1"
                     /translation="MRTCSLCGKKTVILRRRSHAQNQTPRAYRANIQKVTIKVGKDKI
                     SGFFCTKCLKRMKKELNLQQKTASSQK"
     gene            complement(2032..2898)
                     /locus_tag="PK131_00550"
     CDS             complement(2032..2898)
                     /locus_tag="PK131_00550"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPD44657.1"
                     /translation="MVRILFFGSAIYSVIVLKSLLEFKNAKLVGVVTKPDKPKPNPVA
                     QFCHQTHLPLFQTEKFDPEFLKIYQDLNPDLVVVVAFGPPFFTTKMINFPRYKIVNIH
                     PSPLPAYRGAAPAPWQIINGETTSAVTFFVIDELPDHGPIIQQIPFPIDPRETSKSFY
                     QKAFNLAAQNTAKVLSTYCQFPQKIAPQDETKKSYFPRITRDSAMIDWSWEKIKIERF
                     IRAMNDWPIAWTIVTNQKNQQLTMKVFSAELIGNDLTLLEVQIEGKNKTLWKEIEKYY
                     QIDVLNTAIIRE"
     gene            2956..3504
                     /locus_tag="PK131_00555"
     CDS             2956..3504
                     /locus_tag="PK131_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPD44658.1"
                     /translation="MGTIITFPNAALRIKTEEIKEINGKVKKNMEKLRDVLGKCANGV
                     GLAATQLGWSERMFAMKDLKTGQVEVLINPSIIEVWGDKCFPRIINAKGQLEEEPFLE
                     GCLSFPNIFGTVKRYLKIKGKWDGTEKILEGFEAVVFQHELDHLNGILFTDRVRENKG
                     KLYRLEGENREELSLQNYLEQF"
     gene            complement(3407..4042)
                     /locus_tag="PK131_00560"
     CDS             complement(3407..4042)
                     /locus_tag="PK131_00560"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD44659.1"
                     /translation="MKRKALIFIIILFTICGLRFVIRFYLADIAYNRGDFRSAIALNP
                     TEPIYYSRLGLELAQNNDPKAINYSDRAVSISPANLNILKERAQIFLYLTNLDKENYA
                     KSIATLKTITQLAPTDAKIYWTIGQFLNVAGYSKEAIPFYQQAIILKENYDDAHFALG
                     KIYFSLKQFDQAQLELEKTLKIAPNNSEAKALLCSLPQADTISLCSPLPDR"
     gene            complement(4032..5189)
                     /locus_tag="PK131_00565"
     CDS             complement(4032..5189)
                     /locus_tag="PK131_00565"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPD44660.1"
                     /translation="MTCRPWIKFLFSCLICLTPLIFCPFNSELFELPKMYFVYALTLI
                     ILALHLINVVNGHVPLFKHTFLDWPLLLFLLSQIISTIISIDPHTSFFGYYSRLNGGL
                     LSIICYSLLYWILVVYIDQNFKRTLIRLSLICGLVVALYGILEHFGIDKNYWIQDVQN
                     RVFSTLGQPNWLAAYLCLLLPFCFLSPNLLTFTLTIIFSFCLLFTKSKSGLIAAVISL
                     LLCIRKNNFYYYVIFIIITSLFFLGHQSSPPPPSNLNITPSEDIRKIVWTGAIDLYKK
                     YPLFGTGVETFAYTYYWTRPASHNLTSEWEFLYNKAHNEYFNYLATTGTVGIATYLFL
                     ILTVSYQMIKKRQFILLASYLSILITNFSGFSVVVTALFFYLLPAFIIDET"
     gene            5213..6265
                     /locus_tag="PK131_00570"
     CDS             5213..6265
                     /locus_tag="PK131_00570"
                     /inference="COORDINATES: protein motif:HMM:NF010539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPD44661.1"
                     /translation="MPVGNLISKLSWEVGIDLGTCNTLMYLKERGIVINEPTMIARTK
                     KKKWVGLSTPKFNFAKPIAYGYKAKEMLEREPTQIEVVSPIKNGSIQDLNALEKLINY
                     YLKLVREIPYRYPKIFKPKVIVGIATFLNPVHKRAARELLKDEGAGQVILVDQAVLSA
                     INLGVSLETADGVMIVDVGGGKTEITVVSTGGVVVGKSINVAGKSLDQDIINFIKMKY
                     NLTIGQLTAEKIKIQGEGVIRGRDLSSGLPKSIKVSSGEIKEAIGLSLNKIVKTVTRV
                     LDETPPELITGILKQGIVMVGGGAKIEGLAKMIEDEVKIGANVVDEPELSVIKGCGEL
                     IENPSKLSQVKLWAND"
     gene            6258..7778
                     /locus_tag="PK131_00575"
     CDS             6258..7778
                     /locus_tag="PK131_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPD44662.1"
                     /translation="MTKYFFLKAGLIFSLLVLLAITIELSLIRHSYYKSLAIDNKLTE
                     ISLLAPRGRILDRKGREIAVSVYQEDDNLWKRKYPYGQAMGLISGYVNQVNEDDLKER
                     KCGLEFDHMDLIGRGGVEEKMDCSLRGKNGRRLIEVDALGRYVRELGREEPVSGQNLT
                     LSLDAYWQEQAFQLLNGRKAVLLVSNPKTGEVLVMASSPSFDPNNFSFNKNDIAVNNY
                     LRDDSNFPLLNRAIAGRYHPGSVFKPVLAVAGLESGVISGETTFEDTGFIRVGDYTYS
                     NWLWLKERRTEGQVNVVKALQRSNDVFFYKLGEKLGVDQIKQWAVRFGYGLKTGIELP
                     GELAGIVPDAQWKKETKGETWFLGNTYHLSIGQGDLSVTPLQINQMTNIIANDGIKCP
                     MTILALPENEKRSRNEVLGIKKQTLTLVKEGMKRACQKGGTGWPLFGMKTEVACKTGT
                     AEVGDGTNDTHAWMTAFAPADNPEISITVIVERGGEGSDIAGPIVKDFLEKWFENN"
     gene            7784..8353
                     /locus_tag="PK131_00580"
     CDS             7784..8353
                     /locus_tag="PK131_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPD44663.1"
                     /translation="MKLHCLGNKKLLQEKKLIAVVGSRRMSSYGKQVVERIVPELVKA
                     GYIIVSGMAFGIDAWSHKVCLDYKGKTIAVLASGVNMPTPKSNRWIYERIIRENGLIV
                     SQFKNGTIPQPVNFLKRNEVIVDLCQKILIVEGNQTSGTLVTAKLAADQGKDVYCVPG
                     RITDNNSFAPNFLIKNGAQIVTEINDLLI"
     gene            8384..>8612
                     /locus_tag="PK131_00585"
     CDS             8384..>8612
                     /locus_tag="PK131_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012610322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HPD44664.1"
                     /translation="MTVMSKLLIVESPTKARTVSKYLGHDFQVVATVGHLRDLPKSKM
                     GIDMNTFEISYVVDETKRKIIALKRSPFIFSN"
BASE COUNT         2854 a         1262 c         2012 g         2484 t
ORIGIN      
        1 attcatacac ccgattcatc ccgattttgg cgggccgaaa cttataaaga aaggtttaaa
       61 caaggtttag aaccagagaa ttttgataaa gagtttttaa gattgtggta cgctgaacgg
      121 ggctataaag gcggaggcca gccgcctcaa atgagtcggc agttaatggt cgatttatcc
      181 cggcggtatc ttactattta tgagaaatta actggaaaaa agtttaaggc ctatcgctac
      241 ccggttgaag aagcaattag acgagcttgt ttggacataa aaatgtagtg gagcccgcct
      301 ccgtcagcca gctggcggac ttcagcgagg caagggcgga gggtagagga gtcgaacctc
      361 tgaaggcttg cgcccactgg ttttcaaaac cagcgccata accactcggc caaccctcct
      421 gcaatcaatt tctaatttca aatttaaatt aatctgagcc tagggccaga ttcggactgg
      481 cggtctagtg tttacaaaac actcgcttta accagctaag ctacccaggc atagttggat
      541 tatggcaaat ttggtaaaat attactagta ttttgcccgg atagctcagt tggtagagca
      601 tccccttggt aaggggaagg tcatggattc aagttccatt ccgggctcag cttatttgaa
      661 aataaaaacc caccaaaagt ttggttcgat aatggctttt ccacgctaaa gaacgtggtg
      721 gaaggctgcc tgattcgcca aaggcgaatt ttggactcgt cttcctttac agaaggttag
      781 ctagaaaaaa caccttctcg aagatacgct tttggtaggc tatcttaatt ataatttaaa
      841 ttttaaaatt gaaaatgatt aaaaaaacta ccttaattaa agagataaca gataaatacc
      901 ctcaattggc cgttattttg gtccaacaat acggttttca ttgtcttggc tgttgggctg
      961 ctggcgagga gacattggcg gagggagctc tggcccatgg ttattcaccc cgggagatta
     1021 gagaaatgat agcagtttta aacaaaagtt tattatcggg aaaaggagat aggaaatgag
     1081 ggccgtgcct tgaaaaagtg gcaggatgag gagaattaaa ataatcattc ctgatctgcc
     1141 cggaaaaagg tcggggaaga tatgggagcc gtcgagggga tggaagggaa gaaggttaaa
     1201 aacggccaga gagatgttga tgaagataag aataccgaag agaggaatgt aaaagaattt
     1261 ggctatgaga gtaattaaga cagcaaaaat taaattactg actggcccgg cgacagaaac
     1321 taacgctgaa tctcggcggg gatgacggaa gttgtatgga tcgataggta caggtttacc
     1381 ccagccaaaa ccagccagaa aaagcattaa agtaccaaga gggtcaaggt gggctagagg
     1441 attaaggcta aggcgaccag ccgagcgggg agtagggtca ccgagatgat cagcaacata
     1501 agcatgggaa aattcgtgaa tggtgatggc tataattaaa gcaattacac tattgatacc
     1561 atttaaaaaa ttagccattg tggtatagta taccccatga gaacctgcag tttatgtggt
     1621 aaaaaaacag ttattcttcg ccgccgctcc catgctcaaa atcagacacc ccgggcttac
     1681 agggctaaca ttcagaaggt aactatcaaa gttggtaagg acaaaatcag tggctttttc
     1741 tgtactaaat gtcttaaaag aatgaagaaa gagcttaatc tccagcaaaa aacggcatct
     1801 tctcaaaaat gaaaacgccg tttttttaag tgagaattta ttctcaatta ctttttcttt
     1861 ttcttgtttt tagctttttt cttggccata actttcctcc tctaacaaaa gttaaatatt
     1921 cttagccgta aatgtcaaat tactgtttgc cttatttttg gtaaaaaaat gatccattta
     1981 acctgaattt ttatcaaaaa tttatgaatt gtttaaaaac tgatctgatt tttattccct
     2041 aattatggca gtatttaaaa catcaatttg gtaatatttt tctatttctt tccagagcgt
     2101 tttatttttg ccttcaattt gtacctcaag taaggttaaa tcgttaccga taagttcagc
     2161 cgaaaaaact ttcattgtta actgttgatt tttttgatta gtcacaatgg tccaagcaat
     2221 cggccaatcg ttcattgctc taataaacct ttcaattttt attttttccc aagaccaatc
     2281 gatcatagcc gaatctctgg taatacgggg aaaatatgat ttttttgttt catcctgggg
     2341 ggctatcttt tggggaaatt gacagtaagt tgataatact ttggccgtat tttgagccgc
     2401 taaattgaaa gccttttggt agaacgattt tgaggtttcc cgtggatcaa tgggaaaagg
     2461 aatttgttga ataatcggac cgtggtctgg aagttcatcg ataacaaaaa aggtgacggc
     2521 cgaggtcgtt tcaccattaa tgatttgcca tggagctgga gctgctcctc gataagccgg
     2581 taaaggtgaa gggtgaatgt tgacaatttt ataacggggg aaattgatca ttttagtagt
     2641 gaaaaaaggc ggaccgaagg caacaactac gactaaatct gggtttaagt cttgataaat
     2701 ttttaagaat tccggatcaa atttttcagt ttggaagaga ggcaagtggg tttgatgaca
     2761 gaattgagct actggatttg gttttggctt atctggttta gtaaccactc caactaattt
     2821 ggcgttttta aattccaaaa gtgattttaa aacgataact gagtaaatag ccgagccaaa
     2881 aaacaaaatt cgaaccatga tttattataa ccttaaattt tcattgaaaa tgagaaaatt
     2941 tgataaaata gggatatggg gactattata acttttccga atgcggcttt gaggataaaa
     3001 actgaggaaa taaaagaaat caacggaaaa gtaaaaaaaa atatggagaa attaagagat
     3061 gttttgggaa agtgtgctaa tggagtaggt ttggcggcga ctcagttagg ctggtcggag
     3121 agaatgtttg cgatgaagga ccttaaaacc ggtcaggtgg aagttctgat taatccaagt
     3181 attattgaag tttggggaga taaatgcttt cctcggatta ttaatgccaa aggtcaatta
     3241 gaagaggaac catttttaga aggctgtctt tcatttccca atatttttgg gacagttaaa
     3301 cggtatttga aaattaaggg caaatgggat ggaacagaaa aaattttaga gggttttgag
     3361 gcagttgttt tccagcatga attagaccat ttaaatggaa ttctctttac cgatcgggta
     3421 agggagaaca aagggaaatt gtatcggctt gagggagaga acagagaaga gctttcgctt
     3481 cagaattatt tggagcaatt ttaagggttt tttccaactc taattgagct tggtcaaatt
     3541 gtttgagaga gaaatagatt ttacccaaag caaagtgggc gtcatcgtag ttctctttaa
     3601 gaattattgc ttgttgatag aaaggaatag cctccttaga gtaaccggct acattaagaa
     3661 attgacctat ggtccaatag attttagcat cagttggagc cagttgggta attgttttta
     3721 aggtagcgat gcttttagcg taattttcct tatctaaatt agtaagataa aggaaaattt
     3781 gagccctttc cttaagaata tttaaattag ccggagaaat agagacggct cgatcagaat
     3841 agtttattgc tttgggatcg ttgttttgag ctaattctaa accaagtcgg gaataataaa
     3901 ttggttcagt tggattaaga gcgatagctg accgaaaatc ccccctatta taggcaatgt
     3961 ctgccaaata aaagcgaatt acaaatcgaa gaccgcaaat tgtaaaaagg ataataataa
     4021 aaattaaagc tttacgtttc atcgataatg aaggctggta aaaggtaaaa aaatagggcg
     4081 gttactacca cagaaaaacc actgaaatta gtgatcaaaa tggataaata agaggctaga
     4141 aggataaatt gtcttttttt aatcatttgg taggagacag tcaaaataag gaaaaggtaa
     4201 gttgctattc ctactgtgcc agtagtagct aggtagttga agtattcatt gtgggcttta
     4261 ttgtagagaa attcccattc ggaagttaga ttgtggctag ccggtcgggt ccaatagtag
     4321 gtgtaagcga aggtttccac tccggtcccg aataaagggt attttttata gaggtcgata
     4381 gctcctgtcc agacgatttt acggatatct tccgaagggg tgatattaag gttggatggg
     4441 ggaggtggtg aagattggtg tccaaggaag aataaggaag taatgataat aaaaataaca
     4501 tagtaataaa aattattttt cctaatacaa agtaaaagac tgataacagc ggcaattaaa
     4561 ccacttttgg atttagtgaa aagtaaacaa aaactaaaga taatggtcaa agtgaaagta
     4621 agtagattgg ggcttaaaaa gcagaaaggt aagagaaggc agaggtaggc tgccagccag
     4681 tttggctgac caagagttga aaagacccga ttttggacgt cttgaatcca ataattttta
     4741 tcgattccga aatgttccag aatgccgtat aaagctacca ccaacccaca tattaaagac
     4801 agtctgatta aggtcctttt aaagttctgg tcgatataga caaccagaat ccagtatagg
     4861 agactataac aaataatgga gagtaaaccg ccattaagtc ttgaataata gccaaaaaaa
     4921 gaagtatgag ggtcaataga aattatggta gaaataattt gggagagaag aaaaaggaga
     4981 agcggccaat caagaaaagt gtgtttaaaa agaggaacat ggccattaac gacgttaata
     5041 agatgaaggg ccagtataat taaagttaaa gcgtagacaa aatacatttt gggtaattca
     5101 aataattcgg aattaaaggg gcagaaaata agaggtgtca ggcaaataag acaactaaag
     5161 agaaacttaa tccatggacg acatgtcatc aacatatagt atactgaaga gaatgccggt
     5221 tggtaattta atttcaaaat taagttggga agtaggtata gatttgggta cttgcaatac
     5281 tttgatgtat ttaaaggaac gaggaatagt aattaacgaa ccaacaatga tcgccaggac
     5341 aaaaaagaaa aaatgggtgg gtttatcgac accgaagttt aattttgcta agccaattgc
     5401 ttacggttat aaggctaagg agatgttgga gcgggaaccg actcaaattg aagtggtgag
     5461 tccgattaaa aatggtagta ttcaagacct aaacgctctg gaaaaactga ttaattatta
     5521 cctgaaatta gtcagggaga taccttaccg ctatcctaaa atttttaaac caaaagtaat
     5581 cgtgggaata gcgacttttc taaatccggt tcataagaga gccgcgagag aattattaaa
     5641 ggatgaagga gccgggcagg tgattttggt tgaccaagct gttttatcag ctataaattt
     5701 gggggtttca ttagagacgg ctgacggagt aatgattgtt gatgttggtg gaggtaaaac
     5761 agaaatcacg gtggtttcca caggaggggt agtagttggt aagagtatca atgttgccgg
     5821 taaaagttta gatcaagata ttattaattt tattaagatg aaatacaatt taactatcgg
     5881 acaattaaca gcggagaaaa ttaagattca gggtgaaggg gtaattcgag gacgggatct
     5941 ttcctcgggt ttgcctaaaa gtatcaaggt gtcaagtggt gaaattaagg aagctattgg
     6001 tttaagcctt aacaagatcg ttaaaacagt aactagagtt ttggatgaga caccaccaga
     6061 attaataacc gggattttaa aacagggtat cgttatggtt ggtggtggag ccaaaattga
     6121 aggtttggcc aaaatgattg aagatgaagt gaaaattgga gcaaatgtcg tcgatgaacc
     6181 agaattaagt gtaattaaag gttgtggtga attaattgaa aacccgtcaa aattgtctca
     6241 agttaagtta tgggctaatg actaagtatt tttttttaaa agctggatta atcttttctc
     6301 ttttagtttt attggcgatt acgattgaat tatctcttat tcgacatagt tattataaaa
     6361 gtttggctat cgataataaa ttaacagaaa ttagtttatt agcaccgagg ggaagaattc
     6421 ttgatcgaaa agggcgggaa attgctgtta gtgtttacca agaagacgat aatttatgga
     6481 aaagaaaata tccttatggc caagccatgg gtttaatcag tggttatgta aatcaggtca
     6541 atgaagatga tcttaaagag agaaaatgtg gtttggaatt cgaccatatg gatttgatag
     6601 gacgaggcgg ggtggaggaa aaaatggatt gtagcttaag gggtaagaat ggccgccgct
     6661 tgattgaggt agatgcctta ggacgctatg ttagagaatt gggtagggaa gaaccggtta
     6721 gcggtcagaa tttaacctta agtcttgatg cctattggca agagcaggcc tttcaattat
     6781 taaacggtcg aaaagcggtt cttttggtgt ctaatcctaa aaccggggaa gttttagtta
     6841 tggcttcgtc accttctttt gatcccaata atttttcttt taataagaat gatattgcgg
     6901 ttaataatta tttgagggat gatagtaatt ttcctttgct taatcgggct attgccgggc
     6961 gatatcatcc tggctcagtt tttaagccgg ttttagcagt tgctggtcta gagagcgggg
     7021 ttattagtgg ggaaactact tttgaagaca ccggttttat tagggtgggt gattatactt
     7081 acagtaattg gctctggtta aaagaacgaa gaactgaggg tcaagtgaat gtggttaaag
     7141 ctcttcaacg atcaaatgat gtcttttttt acaaattagg agaaaaatta ggagtcgatc
     7201 aaattaaaca atgggcggta aggtttggct acggcctaaa aactggaatc gaattgcctg
     7261 gggaattagc tggtattgtt ccggatgctc agtggaaaaa ggaaaccaaa ggagaaactt
     7321 ggtttttggg taatacctat catctttcta tcggtcaagg agatttaagt gtcaccccct
     7381 tacagataaa tcagatgact aatattattg ccaatgatgg aattaaatgt ccaatgacta
     7441 ttttagcctt accggagaat gagaagagaa gtcgaaatga agttttagga attaaaaaac
     7501 agactctaac cctagttaag gaagggatga aacgggcttg ccagaaggga gggacaggtt
     7561 ggcctctttt tggaatgaaa acagaggttg cttgtaaaac agggacagcc gaagtcggcg
     7621 atggaactaa tgatactcat gcttggatga ctgcttttgc tccggctgat aacccagaaa
     7681 tatcaattac agtcatagtt gaaagagggg gggagggatc agatattgcc ggacctatag
     7741 taaaagattt tttagaaaag tggtttgaaa ataattaaaa attatgaaat tacattgctt
     7801 aggcaataaa aagttattgc aggagaaaaa gctgattgcg gtggtgggaa gtagaagaat
     7861 gagtagttat gggaaacaag tggtggaaag aattgtgcca gaattggtta aggcaggtta
     7921 tattattgtt tctggaatgg cttttgggat tgatgcatgg tcacataaag tttgtttgga
     7981 ttataagggc aaaacaatag cagttttagc tagtggcgtt aatatgccca ctcctaagag
     8041 caaccgctgg atctatgaac gaattatcag agaaaatggt ttgattgttt cccagtttaa
     8101 aaatggaaca atccctcagc cagtaaattt tctaaaaaga aatgaagtaa ttgttgattt
     8161 atgtcaaaaa attcttattg tcgagggtaa tcagaccagc ggtactttgg ttaccgcgaa
     8221 actagctgcc gatcaaggca aagatgttta ttgtgttcct ggtcgaatca ctgacaataa
     8281 ttcatttgct cctaattttt taattaaaaa tggagctcaa atagtgactg aaattaatga
     8341 ccttttaatt tagccgttga cagggttgtt agaataatta acaatgactg ttatgtctaa
     8401 attgcttatt gttgaatcac caactaaagc tcgaacagta tcgaaatatt taggacatga
     8461 ttttcaagta gtggctactg ttggtcattt acgggattta cccaaaagta aaatgggtat
     8521 tgatatgaat actttcgaga ttagttatgt cgtggatgaa actaaaagga aaataattgc
     8581 gttgaaaaga agtcctttta tcttttcaaa tg
//
LOCUS       DAOOIE010000009         7663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_8078_length_7663_cov_3.611330, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000009 DAOOIE010000000
VERSION     DAOOIE010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 7663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7663
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8078_length_7663_cov_3.611330"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="PK131_00590"
     CDS             <1..1093
                     /locus_tag="PK131_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013626097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD44665.1"
                     /translation="HRFNLANLNVANVAIRVNQLIATMMPIIMLLINMSTVAIIWFGS
                     HRVAGGHLEIGAMFAFLQYAVQILFSLLMLAVLFIMIPRAEASAKRINEILELKPDIK
                     DPKKELPATHLRGVVEFKNVTFSYHGAERPALTHITFTASPGRVTAIIGGTGSGKSTL
                     VNLIPRFYDVQEGQILVDAIDVRQMTQATLRAKIGFVPQKPILFSGTVADNIRYGKKN
                     ATLREITHAAEIAQAIEFISKMADGFNAHVAQGGTNLSGGQIQRLSIARALVRQPEIY
                     IFDDAFSALDFKTDAKLRSALKQETQNSTTIIVAQRVSTVMDADQIIVLDNGQIVGLG
                     THKQLLQTCRVYKDIVSSQLSAAEIKESL"
     gene            1090..3003
                     /locus_tag="PK131_00595"
     CDS             1090..3003
                     /locus_tag="PK131_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD44666.1"
                     /translation="MSEATSDHRLKHRGHGGFGARFGRFEKAKDFKGTLKRLAGYFKP
                     EAKRLIFVAIAAILGTVFNILGPKILGKAVTKLFNNLISSFAIQARNQIIKQQLAQNP
                     SLSLQLLPVPGIEWGYIGRIMLTLLVLYLFSALFIFIQQYLMSGVAQRTIYKLRNEVD
                     EKLHRLPLKYFDSRTHGEILSRAVNDMDNLGSTLQQSVSQTITSTTTLIGVLIMMLSI
                     SWRLTLIVLATVPLTLFIVGGIAKRSQIYFRNQQKSLGDLNGHVEEMYTGHKIIKAFG
                     HEETAITEFIELNNRLYRSSWRAQFVSGIILPLTRFVGNLGYILVAVAGGVMATQGMI
                     TLGDVQAFIQYASQFTQPIAQIATITNIIQSAIASAERVFELLDEKEQTPEVTNPVVI
                     KNIKGAVKFDHIYFGYSHDNILIKDMNIDVKPGQMVAIVGPTGAGKTTLVNLLMRFYE
                     VNSGRILIDGVDITQMRRCDLHRLFGMVLQDTWLFHGPIRDNIAYGREGATEAEIIKA
                     AEMAYADHFIRTLPKGYNMVINEEATNISQGQKQLLTIARAFLANPKILILDEATSSV
                     DTRTEQLIQNAMEKLMKGRTSFVIAHRLSTIRNADLILVMNHGTIIEKGTHRELLAAN
                     GFYADLYNSQFSD"
     gene            complement(2993..3520)
                     /locus_tag="PK131_00600"
     CDS             complement(2993..3520)
                     /locus_tag="PK131_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPD44667.1"
                     /translation="MESSLVLSNLLVQGIKIIFILIIAGLIDWLLKTFITKFINSRIK
                     GKMVDGRRKKKAATLIGVFYGIVNFIIWLVVSLMILSEFRVNIAPILASLGIAGLAVS
                     MAAKDIIADFIAGIFILFEGQYYVGDRIKIIDHEGVVEEITLRKTVIRDSHGILYFIP
                     NSQIKIVARQPETNH"
     gene            complement(3540..3626)
                     /locus_tag="PK131_00605"
     tRNA            complement(3540..3626)
                     /locus_tag="PK131_00605"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3590..3592),aa:Ser,seq:cga)
     gene            complement(3734..3807)
                     /locus_tag="PK131_00610"
     tRNA            complement(3734..3807)
                     /locus_tag="PK131_00610"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3771..3773),aa:Arg,seq:gcg)
     gene            complement(3832..4365)
                     /locus_tag="PK131_00615"
     CDS             complement(3832..4365)
                     /locus_tag="PK131_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44668.1"
                     /translation="MKHKDKSEMVMDMNIEKDLYEWVQMDRPFKKRDREFWLTAIAIL
                     VLISIIFIYLKEFLLVVALFSALFLYYTLSTIEPNKIKYRITNRGIYWGDSRMEWGML
                     RRFWIKPSLDSEVINFETNLRFPRQVAVIINPEDREKIKNIIARRIPCLEESPQLTDK
                     VTNWFGKKFPLESKKAS"
     gene            4419..4504
                     /locus_tag="PK131_00620"
     tRNA            4419..4504
                     /locus_tag="PK131_00620"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4452..4454,aa:Ser,seq:gct)
     gene            4661..4789
                     /locus_tag="PK131_00625"
     CDS             4661..4789
                     /locus_tag="PK131_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44669.1"
                     /translation="MTQIEPIEVKEFISELMEQIISHLHQLVLADKRKRIVRYLKT"
     gene            complement(4890..5066)
                     /locus_tag="PK131_00630"
     CDS             complement(4890..5066)
                     /locus_tag="PK131_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPD44670.1"
                     /translation="MATINNEVKQEILTKIKAGDEVKKVAVQYGVYDRTIYVWLKKGV
                     TNNVSVLELGKLRN"
     gene            5475..6077
                     /locus_tag="PK131_00635"
     CDS             5475..6077
                     /locus_tag="PK131_00635"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPD44671.1"
                     /translation="MKVYITRHGESVSNAEGFIAFSYTGLSEKGMVDAKKLGERLARE
                     NIKIDAVYCSSLFRTLQTMDQIFKGGFKIDPKKVFVTDLVREINRAEYEGQTSTDYYA
                     VRDSSGLDPDDFKCKGGESENDVKRRAAEFKKLVEESGYKNILVITHGHFIGQFNSLF
                     GMKLEHTTGGALSLLEIEGDKAEVKFWNDTSHLEESQMSA"
     gene            complement(6051..7205)
                     /locus_tag="PK131_00640"
     CDS             complement(6051..7205)
                     /locus_tag="PK131_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPD44672.1"
                     /translation="MANKNSKLIQNTTVLKGEELEIAKNIDVLVVGDTIKAIEKNINS
                     QDCEIIDGRQFLLCPSFTNAHLHLDDSTILDTGVGQTLEELVFPEGTRDKITSVSKAE
                     TQKAIKSSVSQIIRSGTGLICSFCENGIEGVNLVKECLPPELKSVLVGRFKSKFPYEA
                     KVTDLLNSADGFAPGFIRDIPIEDLQNLSNEVISANGFIAMHSLESKGLPAENFYKTV
                     DYLHPQFLVHLVQATSKETEYLRNKNIGVVCCPRGNSLTGVGFPPILGLLNQDTLVAL
                     GTDNIFLNSPDMFKEMEFTSRLLRGYSQNPSVISSREVLKMATINGAKLLGLGERYGS
                     IEPNKKANLIFFSLNSNNFSSSADYLSTIVHRANSMDIAGISLGGHLTFF"
     gene            complement(7186..7422)
                     /locus_tag="PK131_00645"
     CDS             complement(7186..7422)
                     /locus_tag="PK131_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44673.1"
                     /translation="MTLDTPRRRWIHFAQDYFYYPHVYTHAVCDLADVGIQTRDQTLQ
                     LVTSFCQQRGFDIDLSSSLTHYLETRSSHGQQKQ"
BASE COUNT         2374 a         1510 c         1434 g         2345 t
ORIGIN      
        1 acaccgattt aatttggcta atttaaatgt cgctaacgtc gctatccggg tcaatcaatt
       61 aatagctacc atgatgccga ttatcatgct cttaatcaat atgtcgactg ttgccataat
      121 ttggtttggc agtcatcggg tagctggcgg ccatttagaa attggggcta tgttcgcctt
      181 tctccaatat gctgttcaaa tccttttttc ccttttaatg ttagctgtcc tttttattat
      241 gatccctcga gccgaggctt cagccaaacg aatcaatgaa attctggaat taaaacccga
      301 tattaaggat cctaaaaaag aattaccggc tacccatctt aggggagtag tagagtttaa
      361 aaatgttact tttagctacc atggggccga acgaccagcc ttaacccata ttacttttac
      421 agcttccccc gggcgggtaa ccgccattat tggcggtact ggttctggta aatcaaccct
      481 ggttaacctc atccctcgtt tctacgatgt ccaagaaggc caaattttag tcgacgctat
      541 tgatgttcgt caaatgaccc aagctacttt acgggctaaa atcggcttcg ttcctcaaaa
      601 acccatcctt ttttccggca ctgttgccga taatatccgc tacggcaaga aaaatgctac
      661 tttaagggaa attactcatg cggccgaaat tgctcaagct atcgaattta ttagcaaaat
      721 ggcggatgga tttaatgccc atgttgccca ggggggaact aatctttcgg gtggccagat
      781 tcaacgtttg tcaattgctc gagctttggt tcgtcaacca gaaatttata tcttcgatga
      841 cgctttttct gccttagatt ttaaaacaga tgccaaatta cgttcggcct taaaacaaga
      901 aacccaaaac tcaactacta tcatcgtggc ccagcgggtc agcacggtca tggatgccga
      961 tcaaatcatt gttcttgata atggtcaaat tgtcggttta ggcactcaca aacaattatt
     1021 acaaacttgc cgtgtttata aggatattgt gtcatctcaa ttatcggctg ctgaaataaa
     1081 ggagtcttta tgagcgaagc taccagtgat catcgattaa aacacagagg ccacggtggt
     1141 tttggcgcca ggtttggtag atttgaaaag gctaaggatt ttaaaggaac tcttaaaaga
     1201 ttggctggtt attttaaacc agaagcaaaa cgactcatat ttgttgcaat agcggctatt
     1261 ttaggaactg tttttaatat ccttgggcct aaaatcctag gcaaagctgt cactaaactt
     1321 tttaataatc tcatcagcag ctttgctatc caagcccgca atcaaataat taaacaacaa
     1381 ttggcccaaa atccctccct gtcccttcaa ctcttgcccg ttcccggaat tgaatggggt
     1441 tatatcggcc gaatcatgct cactcttttg gtcctttatt tatttagcgc cctttttatt
     1501 tttattcaac aatatttgat gtcgggtgta gctcagcgga caatatataa actccgcaac
     1561 gaagttgatg aaaaactcca ccgattacca ctgaaatatt ttgacagccg aactcatggc
     1621 gaaattctaa gccgagctgt taatgatatg gacaatcttg gctcaactct tcagcagtcg
     1681 gtctcccaaa caattacttc caccactact cttatcggcg ttcttattat gatgttatct
     1741 attagttggc gtcttacgtt aattgtttta gcaacggtac ccttgactct gttcatagtc
     1801 ggcggtattg ccaaacgctc ccaaatttac ttccgaaacc aacaaaaatc tctgggtgat
     1861 cttaatggtc atgttgagga aatgtatact ggacataaaa ttattaaagc cttcggtcat
     1921 gaagaaacag ctattactga gtttattgaa cttaacaacc gtctctatcg atccagttgg
     1981 cgggctcaat ttgtttccgg cattatttta ccgttaactc gctttgttgg taatctaggc
     2041 tatattttgg tagcggtggc cggcggagtt atggctactc agggaatgat tacccttggc
     2101 gatgttcaag cttttattca atatgcctcc caattcaccc agccgattgc tcaaatagca
     2161 actattacca atattattca gtcagcaatc gcttcagctg aacgggtttt cgaactttta
     2221 gacgaaaagg aacaaacacc tgaagtaacc aatccggtgg ttattaaaaa tattaaagga
     2281 gcagtcaaat ttgaccatat ctactttggc tatagtcacg acaatatcct gattaaggat
     2341 atgaacattg acgttaaacc tggacaaatg gttgctatcg tcgggcctac cggtgccggc
     2401 aaaaccaccc tagtaaatct attaatgcgc ttttatgaag tcaacagcgg ccgtatttta
     2461 attgacggtg ttgacatcac ccaaatgcgt cgttgtgatc ttcatcgcct atttggaatg
     2521 gtcctccaag atacttggct ttttcatggc ccaattagag ataatattgc ctacggccgg
     2581 gagggagcca ctgaggcaga aattatcaaa gcagctgaaa tggcttatgc tgatcatttt
     2641 atccgaactt tacctaaggg ttacaacatg gttattaacg aagaagcaac caatatctct
     2701 caagggcaaa aacaattgtt aaccattgct cgtgcttttc tggctaatcc taagattctt
     2761 attttagatg aagcgacgag cagtgttgat acccggacgg aacaacttat tcagaatgct
     2821 atggagaagc taatgaaagg ccggactagc tttgttattg cccatcgttt atccaccatc
     2881 cgcaatgctg atcttatttt ggtaatgaat cacggaacaa ttatcgaaaa agggacccat
     2941 cgggagcttt tagccgctaa cggtttttat gccgatctct acaatagcca atttagtgat
     3001 tagtttctgg ttgcctagcc actattttaa tctgactatt gggaatgaaa taaagaatac
     3061 cgtggctatc tctaataaca gttttcctca gagttatttc ctcaactaca ccttcatgat
     3121 caataatctt aattctatca cccacataat attgaccctc aaaaagaata aaaataccgg
     3181 cgataaaatc ggcaataata tctttagccg ccatgctgac tgccaaaccg gcaataccca
     3241 aactggctaa aattggagca atgtttaccc taaactctga caaaatcatt aaactaacta
     3301 ccagccaaat tataaaattc actatgccgt aaaaaactcc gattagggtg gcggcttttt
     3361 tctttcttct tccatcgacc atctttcctt tgattctact gttaataaat ttagtaataa
     3421 aagtttttaa caaccaatca atcagcccgg cgatgattaa aataaaaata atcttgattc
     3481 cctggactaa taaattagat aaaactaaag atgactccat aactaattct actctcaact
     3541 ggcggagggt agaggattcg aacctctgcg ggcttgtgcc cactcgcttt cggggcgagc
     3601 gccatcaacc actcggccaa ccctccatca attaatttaa aatttaaaat ttttaatcaa
     3661 tttttattta ctaatttcta attaaaaaaa ataaataaat agaaattaga aattgattag
     3721 aaattaacaa atgtgtacct gagagggatt gaacctccaa ccgctggttc cgcaaaccag
     3781 tgctctatcc aattgagcta caggtacaaa tcagcccaat tataccaaaa tttaacttgc
     3841 ttttttcgat tccagcggga attttttacc aaaccaatta gtaactttat cagttagctg
     3901 gggtgattcc tccaaacaag gaattcttct ggcgataata tttttaattt tttctctgtc
     3961 ttccggatta ataataactg ccacctgccg gggaaaacgc agattagttt caaaattaat
     4021 cacttctgaa tctaaactgg gtttgatcca aaaacgacga agcatacccc actccattct
     4081 ggaatcaccc cagtaaattc cccgattagt aattcgatat ttaatcttat tcggctcaat
     4141 agttgataga gtgtaataaa ggaataaagc cgaaaataaa gccactacta gtaaaaattc
     4201 ttttaaatag ataaaaatta ttgagattaa aaccaaaata gctatggctg tcagccaaaa
     4261 ttctcgatcg cgttttttaa acggtctgtc catctgcacc cattcgtaaa gatctttctc
     4321 aatattcata tccatcacca tttctgactt atccttatgc ttcatatcct ttttcatttt
     4381 aaagcatttt ggcggaattt ggtatactag aaacactagg aaggatgcgg cgaatggtaa
     4441 gccacaggtt tgctaaacct gcgcccgaaa ggacatgagg gttcgattcc ctctccttcc
     4501 gccaaagggt caaagttcta tgcgacccac ttggtatagc agatttctcc taacctgcct
     4561 ttgtttattt tgtgaaatca acatttttcg gtaaagtagc ctgtaataca actagaagat
     4621 agatttgctt ttccgtactt aatctgagag aaaatattac atgacacaga tagaaccaat
     4681 tgaagtaaaa gaatttattt cggagttaat ggaacaaata atttctcatc ttcatcagtt
     4741 agttttggcc gataaaagaa aacggattgt gagataccta aaaacttagc tactatatta
     4801 tctttgagtt gaattgccct gatggccaac tcgtctttaa caagtctgtt agttcccggt
     4861 agaaggcaga tttacttcct ggtctttttt taatttctca acttacccag ttctaaaacg
     4921 gaaacattat tggtaacacc cttttttaac cagacataaa tagttcggtc ataaacacca
     4981 tactgaacag ctactttttt aacttcatca ccggctttaa tcttagttaa tatttcctgt
     5041 ttaacctcat tattaatggt tgccataatt acctcctaaa aagaaaaaat caaatagaat
     5101 aatcactcca gtttaaaagg tacgggtcaa aaacaattgt ctgataacaa tgaagttgat
     5161 tttatattta atactgtggt taaaactatc agagaaacat ttaaaagagt gaatttttga
     5221 aaaatcaatt aattatactt gaatgaatca acaataattg ttgacgaaac ctaaaatttg
     5281 gccggggtca caaaaaatga aattgacaga gtaattatga ccggatgatc aagccttatt
     5341 cctaaattta gacatttaat ggaagaaata tttggaaaga acaaggtaat gcttttcaaa
     5401 ccatttacaa tggtttcgaa aggtttggcg gtaatcagca gtaaaatctt tgcttaagtg
     5461 ctataatttt gaccatgaaa gtatatataa ctcgacacgg tgaatcagtt agtaacgctg
     5521 aaggattcat tgcattttct tacactggct tatctgagaa aggtatggtt gacgccaaga
     5581 aattaggtga aagattggct agagaaaaca tcaaaataga cgctgtttat tgcagtagtt
     5641 tatttagaac ccttcaaact atggatcaga tatttaaagg tggatttaaa atagatccaa
     5701 aaaaagtttt tgtgactgat ttggtaagag aaataaaccg agcagaatat gaggggcaaa
     5761 catctaccga ttactacgcc gtccgtgata gtagtggctt agatcctgat gattttaaat
     5821 gcaagggcgg agagagtgaa aatgatgtta aacgtagagc tgcggaattt aagaaactgg
     5881 ttgaagaatc aggttataag aatatcttgg tcatcactca tggtcatttt attggtcaat
     5941 tcaattccct atttggcatg aagttggaac atactactgg tggagcgtta tcgctgcttg
     6001 aaattgaagg tgataaggct gaagtgaaat tctggaatga taccagtcac ttagaagaaa
     6061 gtcagatgtc cgcctaaaga aattccggca atatccattg agtttgctcg gtggacgatt
     6121 gtagatagat aatcagccga ggacgaaaaa ttattagaat tgagactaaa aaatattagg
     6181 ttggctttct tattgggttc tattgatccg tatcgctcac ctagtccaag taatttagct
     6241 ccattaatgg tagccatttt aaggacttca cgggaactaa ttacggaagg gttttgagaa
     6301 taaccgcgaa gtagtcggga ggtgaattcc atttctttga acatatcggg agaatttaga
     6361 aagatgttat ctgtacctaa agcaactaaa gtgtcttggt ttaataagcc taagatagga
     6421 ggaaaaccaa ctccggtgag agaatttcct ctgggacaac aaacgacacc gatgttttta
     6481 ttgcgaagat actcagtttc ttttgatgtg gcctgaacta gatggactaa aaattgtggg
     6541 tgcagatagt caacagtctt atagaaattt tcggctggta aaccttttga ttcgagagaa
     6601 tgcatggcaa taaagccgtt agcacttatc acttcattgg acaaattctg aagatcttca
     6661 attggaatat ctcttataaa accaggagca aaaccatcgg cactatttag cagatcagta
     6721 actttagcct cataagggaa cttactttta aaacgtccga ctaaaacaga tttaagttct
     6781 ggtggtaggc attctttgac aaggtttacg ccctcaattc cattttcaca aaaactacaa
     6841 attaacccgg ttccacttct gattatctgg gagacagaac ttttaattgc cttttgggtt
     6901 tctgctttgc taactgaagt tatcttgtct ctcgtacctt caggaaaaac taattcctct
     6961 aaggtttgac cgactccagt atctagaatg gtactatcgt ctaaatggag gtgggcatta
     7021 gtaaatgaag ggcaaagtaa aaattgacga ccgtcaatga tctcacaatc ctgagaattt
     7081 atatttttct caatagcctt gatcgtatct ccaacaacaa gaacatcgat atttttagcg
     7141 atttctaatt cttcaccttt taagacggta gtattttgta ttagtttact gtttttgttg
     7201 gccatggctg gatcgagttt ctaaataatg ggtcaaagaa ctagacaggt caatatcaaa
     7261 accacgttgc tgacaaaagc tagtgactaa ctgaagggtt tgatcccgag tttggatacc
     7321 aacatcagca agatcacaaa cagcgtgagt ataaacatga ggataataaa aatagtcttg
     7381 agcaaaatgg atccaacgtc gacgcggagt gtcgagtgtc attcgactct ttttccattc
     7441 ggaatattct cgagttattg gataaaagaa gtcggcatat tggatttcat gatcggcata
     7501 ggcaaagttt ttgggggttg gatctatacc aacttgagtg cctttggttt ggaataactc
     7561 accaaacctt tagagctata tcaatttctt caacacgcag cctcactgct tcattgaatg
     7621 aacgcctact taatggttta cttattcgaa aacttgatga att
//
LOCUS       DAOOIE010000010         6931 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_9070_length_6931_cov_2.987784, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000010 DAOOIE010000000
VERSION     DAOOIE010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6931)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6931)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6931
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9070_length_6931_cov_2.987784"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..418
                     /gene="ssrA"
                     /locus_tag="PK131_00650"
     tmRNA           78..418
                     /gene="ssrA"
                     /locus_tag="PK131_00650"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            433..1416
                     /locus_tag="PK131_00655"
     CDS             433..1416
                     /locus_tag="PK131_00655"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HPD44674.1"
                     /translation="MGKLIKGLVKQIKKLGTKYRKLLLIGLIVYGLILLGIGTFIGSF
                     FNKATLDKFPELNRDSKILIAAPHIDDEVIATGGLIQKAIKAGAEIKIIYLTNGDNNL
                     NSVIVDEDKNLKIGPDDFITLGETRMAEGRQAAKDLGLSDKNLEFLGYPDQGLSQLFN
                     NYYSADKKYSAKGIKFTYNPYAGTYRSKQDYNGENIVNDLSTIMNSFQPNILIIPHPL
                     DQHPDHKATFRFFDKVLAENPKQNNIKVWLYLVHYKNYPADKLVAPNKFLFPPSKLFS
                     QSGWFSLDLDKDEIQVKLKALSENKTQLVKIPLLDLRMFIRRNEIFEIHEF"
     gene            complement(1379..1795)
                     /locus_tag="PK131_00660"
     CDS             complement(1379..1795)
                     /locus_tag="PK131_00660"
                     /inference="COORDINATES: protein motif:HMM:NF013804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HPD44675.1"
                     /translation="MKHFNQDCRDYRFIDKFEAGLVLTGAEAKSLRTQTAQFASAKVE
                     IRQGVPYLENLRLSPYKYAHSQPIDPTRPRQLLLKEKEIAKLISYRRQKYTLLPIRIF
                     LHGKWFKLEIGVGRKIKKYEKREKIKTREFQKFHFV"
     gene            complement(1792..3264)
                     /locus_tag="PK131_00665"
     CDS             complement(1792..3264)
                     /locus_tag="PK131_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44676.1"
                     /translation="MDYSCRFYNGNSSSGQWVHFWEEKKKGRLFGLIWADNAVQGQEI
                     ISVINRRRNLDQISNAAVGLFISHGDYFEAMASQGVYLAVKRGHYFSFLDFSHHQVVR
                     GRGQDGDWWAISNSCDFIKQLFISGATSDIFPPANIAAVLIEINRSRSITHPQYLVRA
                     PVHEAVHRRRLWTIIFSLIGIIILLISLWTIYRHRQINKEMRHQNLITQLETKINQKE
                     TEAAQKLFQDLKEISHDQREVDLYFQKIKKLVNPLELFYDTHLINESSNYSQIVFNGS
                     GLVMFDPINNRLDWLSLSQSKKELDQGDHLQGIKAIITDKNKIYALTASEIRLFDGTE
                     WKKLAPLEKEALDFQIWNNSLYVLYPETIKKLNSLSSWLAAGQQLPVNPAAMAIDGKI
                     WILTDSGELKPYFHGKQDKFNFSQNLVLTGAKSLLTSTMSDDLIFADQNHVYVVSKSG
                     ELKLVKAVEEIKIIDLTADFNSKNLYILASDQKIYIISLP"
     gene            complement(3277..3510)
                     /locus_tag="PK131_00670"
     CDS             complement(3277..3510)
                     /locus_tag="PK131_00670"
                     /inference="COORDINATES: protein motif:HMM:NF040135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44677.1"
                     /translation="MDPLTNNLNLNNIFFLLTKIFVIVGGLIYLIFALLIVKQVKVFS
                     KNIRDKFNRPIMIASYLHLILAFLLIFLALLFL"
     gene            complement(3491..4150)
                     /locus_tag="PK131_00675"
     CDS             complement(3491..4150)
                     /locus_tag="PK131_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44678.1"
                     /translation="MTIRAGNLDTIAPDDKIIKTEKNENVQEDLTPIRRILGHTRNSF
                     SSTLICPNFFDFSERSEGEIIYVVLRPHWFTNISWILLSLVMFLAPLILKLLAVEISL
                     PGNLFLLIIIFWYMVTLAFALEKFISWYFDLSIITNLRVIDMDIFNLLNRKFTEAEIK
                     NIQDIDYRLAGASQTFFNYGSVRIETAGERPNLVFEKIAAPDKILKLLQELCHHGSFN
                     Q"
     gene            complement(4180..5883)
                     /locus_tag="PK131_00680"
     CDS             complement(4180..5883)
                     /locus_tag="PK131_00680"
                     /inference="COORDINATES: protein motif:HMM:NF020075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPD44679.1"
                     /translation="MVRRVINIWFIFFVFLFVLPFPCYADLDSDITKLGEQIAQIDAA
                     LSPLKKESSDLTKKINVAKSQIALAEKQIEALTADIEEKAAELANQKTLLGERARRYY
                     INSRKFDPLMIFLASNESSSLIRQYNWYQSIIAKDRDLIMTYTNDLSVLNQNKANLEA
                     QKIKLAGLKKSLESRFGFLAGEIQKAEDYKAELTAKQQALIAEKTAVFNTAVGDVSTA
                     DDPAARADFNPGFSPAYAVFSFGAPHRKGMSQYGAFGRAKAGQNYETILKAYYGDIRL
                     EKIDVPGSIKTSIGTLPFEDNYLIGIAEMPAKWGELGGMEALKAQAIAARTYALNYTN
                     MGSGSICVTETCQVYSRSRYDSPGKWKEAVETTRGLVIKANSTGRIFSTMYASTAGGY
                     TYGYSSSDYATPSVWDTTCGSQGCWPGDAYEKAAGSPWFYKGWYKTRSGTSCGRSHPW
                     LTKDEFADIINANIYFKNTHDSSHLSQYNSGGCFGGNDPNAWGMEELRRQVGDKGGPV
                     SSIDSISVDYSTGGFTKTVHIVTDKGSFDFDGGDFKQVYNLRAPGAIAVKSSLFNIEK
                     K"
     gene            5913..>6931
                     /locus_tag="PK131_00685"
     CDS             5913..>6931
                     /locus_tag="PK131_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HPD44680.1"
                     /translation="MKRLMRWVFMFMFLVFLGGSEAYATCEDYPGTGNLGENCCLLEL
                     NTGWFGKKYKSGQCNTDLYCNLETGKCVEAKGVCGKAVGGECCNWVYPYAGSCDSELG
                     LSCDTKAGKCQRKPGGTTTEPWGDPGKCQYIRDTNSSDKECKLIDQCNYRNGWMGIEL
                     SDPLQRDLYPNCPQCTCGKNPHYQLDTCQVYPFDQYRIMTKFMFCGPGMEPEIYLSAN
                     NTTNCRCIVEGSIHNIDYVGDKLQKYDKEWFSKASNSVISGVGVRLFCEPIDKNNPKV
                     YTALGCLPVSVTGFTAWIIPNLFGIIGGICFLVLVYGFIMVATSAGDPKKTQAAKETI
                     TAAITG"
BASE COUNT         2279 a         1314 c         1302 g         2036 t
ORIGIN      
        1 atacttggtt aggaaagaat tttgttcaaa ttgtgaatat ttgcaaaaat agggagaaag
       61 tgatataata ttgtgttgga gatgtaaagc ttcgacagag attgttcttt aaaagacggc
      121 aggccggctt cgatcccaca gccgaaaaac agggattaaa aataaaatgc catgaattat
      181 tctggcgcca attacgcttt agcttacgct taagccattg gccattcctt ctttgcctat
      241 tccggagaaa agaaaggagt gtcaaccaac cggaatgctt tttcttcaag ggcagcattg
      301 aggaaaatta agattcgggt aagaccgaaa ttagctgtct ttttggaaac aaaataagct
      361 tgtagatgtc ttttttagtc atctttggac ctcggttcgt taccgagcat ctccgctaaa
      421 gtaaaataga atatgggtaa gttaattaaa gggctggtaa aacagataaa aaaactagga
      481 acaaaatata ggaaactgct tttaatagga ttaattgttt atggtcttat tctgttggga
      541 atcggtactt tcatcggtag tttttttaac aaagctactt tagataaatt tcctgagcta
      601 aaccgagaca gtaaaattct aattgcggct cctcatattg acgatgaagt tatcgctaca
      661 ggcggtttaa ttcaaaaagc aataaaggct ggagcggaaa ttaaaattat ttacctaact
      721 aatggcgata ataaccttaa ttcggtcatt gttgacgaag acaaaaatct taaaatcggt
      781 ccagatgact ttatcacttt aggtgaaacc agaatggccg aaggccggca agcagctaaa
      841 gatctgggtt tgtcagataa gaatctggaa tttcttggct atcccgacca aggcttaagt
      901 caacttttca ataattatta cagcgctgat aaaaaatatt ctgccaaggg aattaaattc
      961 acttataatc cttatgccgg tacttaccgt agtaaacagg attataacgg tgaaaacata
     1021 gttaacgatc tgtcaacaat catgaacagt tttcaaccta atattcttat tatcccccat
     1081 ccccttgatc aacatcccga ccataaagct actttcagat tttttgataa agttctggcc
     1141 gaaaacccca aacaaaacaa tattaaagtt tggttatatt tggttcacta taaaaattac
     1201 ccggctgata aactggtcgc tcccaacaaa tttttatttc cgccatcaaa attattcagt
     1261 caaagcggct ggtttagtct agacttagat aaggatgaaa tccaagttaa actcaaagct
     1321 ttaagtgaaa ataaaaccca actagttaaa atacctcttc tcgatttaag aatgtttatt
     1381 agacgaaatg aaatttttga aattcacgag ttttaatttt ttcccttttt tcgtattttt
     1441 taatctttcg accaacacca atttccagtt taaaccactt gccatgaaga aaaattctta
     1501 tcggtaaaag ggtatatttc tgccgacgat aagaaatcaa tttagcaatt tctttctctt
     1561 ttaataaaag ctgtcgagga cgggtgggat caatcggctg actatgagca tatttatagg
     1621 gagataaacg aaggttctcc aaataaggta ctccttgacg aatttccacc ttagcactag
     1681 caaactgagc tgtttgggtc cgcagtgact ttgcctcagc cccagttaac actaggccgg
     1741 cttcaaattt atcgataaat cgatagtcac ggcaatcttg attaaaatgt ttcatggaag
     1801 gctaattata tagatttttt ggtcggaggc caaaatataa agatttttac tattaaaatc
     1861 agccgttaag tcaattattt ttatttcttc aaccgctttg actaacttta attcacctga
     1921 tttactaact acataaacat gattctggtc agcaaatatc aaatcatcac tcattgtgga
     1981 ggtcaacaaa cttttagcac cggtcaaaac caaattttgg ctaaaattaa atttgtcctg
     2041 tttaccatga aaataaggct ttaattcgcc cgaatcagtc aaaatccaaa ttttaccatc
     2101 aatagccatg gctgctggat taaccggtaa ttgttgacca gcagctaacc acgaagataa
     2161 gctattcaat tttttaatcg tttcagggta taaaacataa agactattgt tccaaatctg
     2221 gaaatccaga gcttcttttt ctaatggggc taattttttc cattctgtcc catcaaatag
     2281 acgaatttca ctggcagtta aggcataaat cttatttttg tcagtaatta ttgcctttat
     2341 tccttggaga tgatcacctt gatccagttc tttcttgctc tgggaaagag acagccaatc
     2401 caaacgatta ttgatgggat caaacataac caaaccggaa ccattaaaaa ctatttggga
     2461 atagttactc gattcgttta ttaaatgagt gtcatagaaa agttctaaag ggttcaccaa
     2521 cttcttaatt ttttggaagt acaaatctac ttccctttga tcatgggaaa tttcctttaa
     2581 gtcttggaat agtttttgag cggcttctgt ttctttttga ttaatttttg tctctaattg
     2641 agtaattaaa ttctgatgtc tcatctcttt attaatttgc cggtggcggt aaatagtcca
     2701 taaactaatc agcaaaataa taattccaat taaactaaaa ataattgtcc ataatcgacg
     2761 acgatgaacg gcttcatgaa ctggcgcccg cactaaatac tgaggatgag taattgatcg
     2821 ggaacgatta atttcgatca aaacagcggc tatattggcc ggaggaaaaa tatcagaagt
     2881 ggcccctgaa ataaaaagtt gtttaataaa atcacaggaa ttacttatgg cccaccaatc
     2941 accatcttgt cctcggcccc gaaccacttg atgatgggaa aaatcaagga aactaaaata
     3001 atggcctcgt tttaccgcca aataaacacc ttgacttgcc attgcctcaa aataatcacc
     3061 atggctaatg aaaagaccaa cagcggcatt actaatttga tctaaatttc gccggcgatt
     3121 aataacacta ataatttctt gcccttgaac agcattatca gcccaaatta aaccaaataa
     3181 acggcccttt ttcttctctt cccaaaaatg aacccactgg ccgctgctcg aattaccatt
     3241 gtaaaaccgg caggaataat ccattaaata atgatactac aaaaacagaa gggccaagaa
     3301 aatcaacaga aaggccaaaa taagatgaag ataagaagca atcataattg gccggttgaa
     3361 cttatcacga atatttttag aaaaaacctt aacttgttta acaattaata aggcaaaaat
     3421 taaataaatt aatccgccga caataacaaa gatcttagtc agaagaaaaa aaatattatt
     3481 aagattaaga ttattggtta aaggatccat gatgacaaag ttcctgtaat aattttaaaa
     3541 tcttatctgg ggcggcaatc ttttcaaaaa ctaaattagg ccgttcaccg gctgtttcaa
     3601 ttctcaccga accatagtta aaaaacgtct gactggctcc tgctaatcta taatctatat
     3661 cctgaatatt ttttatttcc gcctcagtaa atttacgatt gagcagatta aaaatatcca
     3721 tatcgattac ccgcaaatta gtaatgatac ttaaatcaaa gtaccaggaa ataaattttt
     3781 ccaaagcaaa ggctaaagta accatatacc aaaaaataat aataaggaga aataaattac
     3841 caggtaaaga gatctcaacc gctaataatt ttaaaatcaa gggagctaaa aacattacca
     3901 aactcaacag aatccaagaa atgttagtaa accaatgagg acgaagaact acataaataa
     3961 tctcgccctc gcttcgctca ctaaagtcaa aaaaattagg acaaatcaga gttgaggaaa
     4021 atgaattacg agtatgacct aaaattctcc tgataggagt taagtcttct tgaacatttt
     4081 catttttttc tgttttaatt atcttatcat cgggagcaat cgtatccaaa ttaccggctc
     4141 gaatcgtcat aaaaatattt taacatggtc aaagactaat tatttctttt cgatattaaa
     4201 cagggacgat ttaacagcaa tggcaccggg agctcgaaga ttataaacct gcttaaaatc
     4261 gccgccgtca aaatcgaaac tacccttatc ggtaactata tgaacagttt tggtaaagcc
     4321 gccggtagaa taatcgaccg aaatggaatc gatcgaagaa accggtccgc ccttatcgcc
     4381 tacctggcgt ctcaattctt ccattcccca ggcattagga tcgttgccgc cgaaacaacc
     4441 gccggaatta tactgggaaa gatgagaaga atcatgagta tttttgaagt aaatattggc
     4501 attgataata tcggcaaatt catctttagt cagccaagga tggcttcggc cgcaactagt
     4561 tccgcttcgg gttttatacc aacctttata aaaccaaggg gagccggccg ccttctcgta
     4621 ggcatcaccg ggccagcagc cctgactacc acaggtagtg tcccaaacac taggggtagc
     4681 gtaatcactt gagctataac cataggtgta tcccccggca gtcgaggcgt acatagtcga
     4741 gaaaatccgg ccggtagaat tagctttaat caccaggccc cgggttgtct caaccgcctc
     4801 tttccacttt ccaggagaat catagcggga gcgggaataa acttggcagg tttcagtaac
     4861 gcagatgcta cctgaaccca tattggtata attcaaggca taagtgcggg cggcgatcgc
     4921 ctgagctttt aaagcttcca tgcctcctaa ttcaccccat ttagccggca tctcggcaat
     4981 accaatcaaa taattatcct caaaaggcag ggtcccgata ctggttttaa ttgaaccagg
     5041 gacatcaatt ttttccaaac gaatatcgcc ataataggcc tttaaaatag tctcgtaatt
     5101 ttggccggct ttagctcggc caaaagcccc atattgagac atgcctttgc ggtgaggtgc
     5161 tccgaaagaa aaaacggcat aggccggaga aaagccagga ttaaaatcag ctcgagcggc
     5221 cggatcatcg gcggtcgaaa catcaccaac agcagtattg aatacagctg tcttttcagc
     5281 aattaaagcc tgttgtttcg ccgttaattc cgccttataa tcttcggcct tttggatttc
     5341 accagccaaa aaaccaaaac gagattcgag actttttttt aacccagcca gttttatttt
     5401 ctgagcctca agattggcct tattctgatt caaaacagac aaatcattgg tataagtcat
     5461 aatcaaatcc cgatctttag caataattga ttgataccaa ttatactgac ggattaatga
     5521 ggagctttca ttgctggcta aaaaaatcat cagcgggtca aacttacgac tattgatata
     5581 gtagcggcgg gcccgttcgc ccaaaagagt tttttgatta gccaattcag ccgctttttc
     5641 ttcaatatcg gccgttagag cttctatttg cttttcagcc aaggcaattt gagacttagc
     5701 aacgttaatt ttttttgtca gatcagaact ttcttttttt aatggggaaa gagcggcgtc
     5761 aatctgggca atttgttcac ccagtttagt aatatcacta tcaagatcag cgtagcacgg
     5821 aaaaggtaga acaaataaaa agacgaaaaa tataaaccag atatttatca cccgtctcac
     5881 cattcaattt taacagatga tatactgata tcatgaaaag attgatgagg tgggtgttta
     5941 tgtttatgtt tttggtgttt ttgggtggaa gtgaggcata tgctacttgt gaagattatc
     6001 ctgggacagg taatttaggc gagaattgtt gtttactaga actgaatact ggttggtttg
     6061 gaaaaaagta taaatctggt caatgtaaca ctgatcttta ttgtaatctt gagacaggaa
     6121 aatgtgttga agcaaaaggt gtatgcggaa aagcagtggg gggtgaatgt tgtaattggg
     6181 tttatccata tgcaggtagt tgtgatagtg aattggggtt gtcgtgtgat actaaggccg
     6241 gaaaatgtca aagaaaaccc ggtggtacaa ctacagaacc atggggtgac cctggaaagt
     6301 gtcaatatat aagagatacg aattcttcag acaaagagtg caaattaatt gatcaatgta
     6361 attataggaa tggatggatg ggtatagaat tatcagatcc attacaacgg gatctttatc
     6421 caaattgtcc acaatgtacg tgtggaaaaa atccacatta tcaattagat acgtgccagg
     6481 tttatccgtt tgatcaatat agaataatga caaaatttat gttttgtggt ccaggaatgg
     6541 aaccagaaat atatctttct gcaaataaca ctacaaattg tcgttgtatt gtggaaggaa
     6601 gtattcataa tatcgattat gttggggata agttgcaaaa atatgataaa gaatggtttt
     6661 caaaagcaag taattctgtt ataagtgggg taggagtaag gctgttttgt gagccgattg
     6721 ataaaaataa ccccaaagtc tacactgctc tcggctgtct gccagtgtcg gtcaccggtt
     6781 ttaccgcctg gattattcca aatttatttg gtatcatcgg cggaatttgt ttcctagttc
     6841 tagtctacgg ctttattatg gtggccactt cggccggtga tcctaaaaaa actcaggcag
     6901 ctaaagaaac cataacggcg gcaattaccg g
//
LOCUS       DAOOIE010000011         6764 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_9358_length_6764_cov_3.306454, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000011 DAOOIE010000000
VERSION     DAOOIE010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6764
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9358_length_6764_cov_3.306454"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /gene="ssb"
                     /locus_tag="PK131_00690"
     CDS             complement(<1..294)
                     /gene="ssb"
                     /locus_tag="PK131_00690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPD44681.1"
                     /translation="MSNRCLNRVTLIGNLTRDPELRYTPAGMAVVSFGLATNRSWVTK
                     QGEKKEDAQFHRIVAWNKLAELCSQLLVKGRRVYVEGKIQYRDFTDRDNNKKQV"
     gene            complement(299..586)
                     /locus_tag="PK131_00695"
     CDS             complement(299..586)
                     /locus_tag="PK131_00695"
                     /inference="COORDINATES: protein motif:HMM:NF013419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPD44682.1"
                     /translation="MITQKEKYQLVLILEPKLESRDQEALFEKIGEKLGKIEKKDHLG
                     IKDMRYKIKGWEKGDYWVLEIGEKMCLTMKEINLFLNRETKIIRYLLLKRR"
     gene            complement(593..1948)
                     /gene="cysS"
                     /locus_tag="PK131_00700"
     CDS             complement(593..1948)
                     /gene="cysS"
                     /locus_tag="PK131_00700"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPD44683.1"
                     /translation="MKVYNTATRKKEKLKTIKPGEVGIYACGPTVYSAPHIGNMYAYI
                     CWDVLVRAWRYLGYRVKYVMNVTDIGHLVSDADEGEDKMEKKAREEGLTVRQIADKYE
                     KQFFDDEAKLNIVRPDIVCRATDYIQDQIKLIQKIEQNGFTYRISDGIYFDTSRFSDY
                     GAFARLDLQQLKEGARIGVNPEKKNQTDFALWKFSLSTNGLTRQMEWDSPWGRGVPGW
                     HVECTAMSIKNLGFPFDVHVGGVDHIAVHHTNEIAQSYGAFGRQAVNYWLHNGFVTFK
                     GEKVSKSKGGLWTITELEKMGFEPLAYRYLVLTSHYRSGLDFSLVNLKTAQTSLNKLR
                     NLIKVKKGKFNKKYLACFKQFLENDLAMPEIIALVWQIVKEEPATVLEMDKVLGLNLA
                     KNKAMNIPEEILELVNQRRQARAVKDWVKADKLREMIRVRGYDVEDTRNDCKIKKLML
                     I"
     gene            complement(1966..2379)
                     /locus_tag="PK131_00705"
     CDS             complement(1966..2379)
                     /locus_tag="PK131_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44684.1"
                     /translation="MNNKQGPIDLGIEMGEKIVQSTDKVITAIKVDELLGDIKPLTDE
                     ELQKKKKEEEKMKAGLKGNDLESQIKRLREIEFRKQTEEKEIEEKKKHDQEVSKEEET
                     IEIPTNPHKMKRKRGSAFIPADKKSQTAEFAKKPD"
     gene            complement(2372..2974)
                     /gene="recR"
                     /locus_tag="PK131_00710"
     CDS             complement(2372..2974)
                     /gene="recR"
                     /locus_tag="PK131_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPD44685.1"
                     /translation="MGNYPKAIKDLIEDFEKLPGVGPKSATRLAFCLLNWPETAIIKF
                     AADLKGIKSVKICQECFGVAETNICGICANPRRDKQVICVVERSIDLLAIENMGEFTG
                     VYHVLGGVINPLNHVGPDDLKIKELIDRLNQGVKEVIIATNPTMEGEATALYIKKKIE
                     ASGFQIKTSRIGSGLPIGADLEYADLATLSRALTGRKPYE"
     gene            complement(2977..3225)
                     /locus_tag="PK131_00715"
     CDS             complement(2977..3225)
                     /locus_tag="PK131_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44686.1"
                     /translation="MFDKAKLLTKALKMKKMIESETTAIEEGGIRIVVSGDQRIKQLS
                     INGVENRLLMDVINKAMKKSQETAARKVKEAGGLEGLF"
     gene            complement(3243..4661)
                     /gene="dnaX"
                     /locus_tag="PK131_00720"
     CDS             complement(3243..4661)
                     /gene="dnaX"
                     /locus_tag="PK131_00720"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPD44687.1"
                     /translation="MTVYHLQYRPQKLGELDLDNLGEKLKKLFSGKETPQSFLLSGPK
                     GAGKTSVARIIARLVNCLAPENGEPCDQCENCLAILKGSNLDVIEIDAASNRGIDDIR
                     NLKESSYLMPSKLKKKVFIIDEVHMLTKEAFNALLKVIEEPPSHVVFILCTTDEEKIP
                     ETILSRLVKIEFRKGTLKELARSLKRIVKGENLDVDDTSLELLASQSDGSFRNLQKSL
                     NEIVMEYGKKISRENVEDYIASKFGNYSGEEIEADLNAGNVKVILDKLEKLAQKGINF
                     TSLRDNWLAYFHQRLLKERSDTLSNWINLLIKTSQLEKGTIIEQLPLELAVIDYLKNK
                     PVISTGTTEDKETNVANKTENPKSNLGLIEQEWKQILAEVKPFNHSVEAFLRAARPKK
                     LEGSVLVLEVFYPFHKERLEEEKNRKVLEDCIAKVIGQQLRVEYVLGKDKGKPLEIKN
                     DTPMEAVYQEHDVYDLAKDIFG"
     gene            complement(4668..5015)
                     /gene="ybeY"
                     /locus_tag="PK131_00725"
     CDS             complement(4668..5015)
                     /gene="ybeY"
                     /locus_tag="PK131_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPD44688.1"
                     /translation="MNKILIKHPKNWGLSENLVRRIAQATLKGWDRVELSLVFVGRQK
                     AKELNQQYRQMNYIPQVLSFPISKEKNADGWIRLGDIVICTEKLKYEAKFQHKSLTDI
                     LAFWLSHGVANLL"
     gene            complement(5008..5445)
                     /locus_tag="PK131_00730"
     CDS             complement(5008..5445)
                     /locus_tag="PK131_00730"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HPD44689.1"
                     /translation="MLKDKLKADSVTALKSGDNLKVERLRYLISLIDQKELTLPMGQL
                     TETDEIMVLQKELKNKEESRLLFLKGKRLDLVTALDEEIELLKSYLPQMMTVEEITAL
                     VGEMRANLGNDFGAIMRAVKNQTGGRASGELIARIIKEQLSGE"
     gene            complement(5432..5650)
                     /locus_tag="PK131_00735"
     CDS             complement(5432..5650)
                     /locus_tag="PK131_00735"
                     /inference="COORDINATES: protein motif:HMM:NF013342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPD44690.1"
                     /translation="MALIVVRKKGETKDDLIGRFRRLSLEEDVSEEIKERTAYRKPSE
                     RRYAKKKIILWREKCRRRAKQSRRYAQR"
     gene            complement(5667..6341)
                     /locus_tag="PK131_00740"
     CDS             complement(5667..6341)
                     /locus_tag="PK131_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44691.1"
                     /translation="MKGAVKVKTVLVIVILLLVGVLVVVGLGAVRTYMAGAAADSGPK
                     DVRAMVSSDGKSATISFTTGKKCQPTVLYGTGPTTMLLSKSAAEEGTDFNITLSPLKD
                     NTLYYYTIKINDTVYDNAGIPYSFSNSVQTKEIVEPTPTVEAVPLVSPVVSPTATDSS
                     SASCNQTTDYNNDGVINSVDYYDCLKGVKVAPTKSTDACVEVDYNKDGVINAVDQLLC
                     RQNSKQ"
     gene            complement(6338..>6764)
                     /locus_tag="PK131_00745"
     CDS             complement(6338..>6764)
                     /locus_tag="PK131_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HPD44692.1"
                     /translation="IYPKVEGEKITVTLVKDKATDGLESTAVAGDLFTIKFKGKETGT
                     AKATFSCTPSSLTDSNLLTTETGTVVDVISCADNQAGSYEITVSGNSSSPTNTPTQST
                     PAETTSTPELPKTGNPAATIGFFLLGGIGLISAFILKAL"
BASE COUNT         1901 a         1606 c          966 g         2291 t
ORIGIN      
        1 tacttgcttt ttattattat cacgatcggt aaaatcccga tattggatct tcccttcaac
       61 ataaacccgc cggcctttaa ctaaaagttg ggaacaaagt tcagctaact tattccaagc
      121 aacaattcga tgaaactggg catcctcttt cttttcacct tgcttggtca cccacgatct
      181 gttggtagct aaaccaaagc ttaccaccgc cattcccgcc ggggtataac gcaactcagg
      241 gtcacgggtt aaattaccta ttaaagttac ccgattaaga catcgattag acatattctt
      301 atctcctttt taacagtaaa taacggataa tctttgtttc acgatttaaa aaaaggttaa
      361 tttctttcat tgttagacac atcttttcac ctatttctaa aacccaataa tcccctttct
      421 cccagccttt aattttatac cgcatatcct taattcctag atgatctttc ttttcaattt
      481 tacctaattt ttcacctatt ttttcaaata aagcttcctg atcccggctc tctaactttg
      541 gctccaagat taaaactaat tgatattttt ctttttgggt tatcattgtt ttctagatta
      601 acataagctt ctttatttta caatcatttc gggtatcctc gacatcataa cctctaaccc
      661 taatcatctc tctcaattta tcggccttta cccaatcttt aacagctcta gcctgccgac
      721 gctgatttac tagttctaaa atttcttcag gaatgttcat agccttattt ttagccaaat
      781 ttaaccctaa aactttatcc atttctaaga ctgttgctgg ctcttctttc actatttgcc
      841 agactaaagc aattatttct ggcatggcca gatcattctc aaggaattgt ttaaaacaag
      901 ccaaatattt cttgttaaac ttacctttct ttactttaat caaattccgt aacttattga
      961 gggaagtttg agctgttttc agattgacta aagaaaaatc caaaccactt cgatagtgag
     1021 acgttaaaac caaatatcta taagctaacg gttcaaaacc catcttttcc agctcagtaa
     1081 tcgtccacaa cccacctttt gatttactaa ccttctcacc cttgaaagta acaaaaccgt
     1141 tatgcaacca ataattaact gcctgcctgc cgaaagctcc ataagactgg gcaatttcat
     1201 tagtgtgatg gacagcaata tgatctacac cgccgacatg aacatcgaaa ggaaaaccta
     1261 aatttttaat cgacatagca gtacactcta catgccaacc aggaacacct cgcccccaag
     1321 gactatccca ttccatttgc cgggttaaac cgttagttga tagactaaat ttccataaag
     1381 cgaaatcagt ctggtttttc ttctcaggat taacacctat ccttgctcct tcttttaatt
     1441 gttgtaaatc taatcgggca aaagcaccat aatcagaaaa acggctggta tcaaaataaa
     1501 ttccatcgct aattctatag gtaaaaccgt tttgttctat cttttggata agtttaatct
     1561 gatcctgaat ataatcagta gcccgacaaa caatatctgg ccgaactata tttaactttg
     1621 cctcatcatc aaaaaactgt ttttcatact tatcggcaat ttgtctaact gttaatccct
     1681 cctctcgggc ctttttctcc attttatcct caccttcgtc agcatccgaa accaaatgac
     1741 cgatatcagt cacattcatc acatatttca ctcgataacc caaataacgc caggctctca
     1801 ccaaaacatc ccagcaaata taggcataca tattgccgat gtgtggtgcc gaataaaccg
     1861 ttggtccaca ggcgtaaata cccacttctc ccggttttat tgtttttagc ttttcttttt
     1921 ttctggtggc cgtgttataa actttcattt attatttacc aattactaat ccggtttttt
     1981 agcaaattct gctgtttgtg attttttatc ggccggaata aaagcactgc ctcttttcct
     2041 tttcatttta tggggattgg tcggtatctc gatggtttcc tcctctttac tgacctcttg
     2101 gtcatgtttt ttcttttcct ctatttcttt ttcctccgtc tgctttctga attcaatttc
     2161 tcttaatcgc ttaatctgac tttccagatc atttcctttt aatccagcct tcatcttctc
     2221 ctcttccttt ttcttttttt gtaattcttc atcagttaat ggcttaatat ctcccaataa
     2281 ttcatccact ttaatggctg ttatcacctt atctgtagat tgaactattt tttcccccat
     2341 ttctatacct aaatcaatag gaccttgttt attattcata tggtttcctc ccggtgaggg
     2401 cccgcgatag ggtagctaaa tcggcatatt ctaaatcagc acctattggc aagccggagc
     2461 caattcgact agttttaatt tgaaaaccac tagcttcaat ctttttttta atatacaaag
     2521 cagttgcctc tccttccatg gtgggattag tagctataat cacttcttta actccttgat
     2581 ttagtctatc aatcagctct ttaattttta aatcatcggg acccacatgg ttgagaggat
     2641 taattacgcc gcctaaaaca tgataaaccc ctgtaaattc acccatattt tcgatagcca
     2701 atagatcaat tgacctctcg acgacgcaga ttacctgttt atcccgccga gggttagcac
     2761 atatcccaca gatatttgtt tcagctaccc caaaacattc ctgacaaatc ttaacacttt
     2821 taattccttt taaatcggcg gcaaacttta ttattgctgt ttctggccaa tttaagagac
     2881 agaaggctaa tcgggtagcg cttttagggc ctactcccgg taatttttcg aaatcctcaa
     2941 taagatcttt aatcgctttt ggataatttc ccataactaa aacaaacctt ccagtccacc
     3001 tgcttcctta actttgcgtg ccgcagtttc ctgcgacttt ttcatagcct tgttaataac
     3061 atccattaaa aggcgatttt ctaccccatt aatcgacaat tgtttaattc gctggtcacc
     3121 actaaccaca atcctaatac ctccctcctc tatagcagtc gtttctgact caatcatttt
     3181 tttcattttc agagctttgg taagtagttt agctttgtca aacatatgtt gtaattataa
     3241 cattaaccaa agatatcttt agccaagtcg taaacatcgt gctcttgata aaccgcctcc
     3301 attggcgtgt catttttaat ctctaaaggt ttacctttat ccttacccaa aacatattct
     3361 acccgcagct gttggccaat aactttggca atacaatcct ctaaaacttt tctattcttt
     3421 tcttcttcca atctttcttt gtggaaggga taaaacacct ccaagaccaa aactgaccct
     3481 tctaattttt taggtctggc cgctcgcaaa aaggcttcaa cgctatgatt aaaaggttta
     3541 acctcggcca agatttgctt ccattcctgc tcaatcaatc ctaaattact ttttggattc
     3601 tcagtcttat tagccacgtt cgtctcttta tcctcggttg ttccggtcga aataacaggc
     3661 ttatttttaa gataatcaat aacggccaat tctaaaggca attgctcaat aatcgtcccc
     3721 ttttctaact gactcgtttt aattaataaa ttgatccaat tacttaaagt atcactcctt
     3781 tccttcaaaa gcctttggtg aaaataagcc agccaattat cacgcaaact agtaaaattt
     3841 attccctttt gggctaattt ttccaattta tcaagaataa ccttaacgtt accggcattt
     3901 aaatcagcct ctatttcctc accactgtaa ttgccaaatt tactggcaat ataatcttca
     3961 acattttcac gactaatttt tttaccatat tccattacaa tttcattaag gcttttttgt
     4021 aaatttctaa aactaccatc actttggctg gctaaaagct ccaaactagt atcgtcgaca
     4081 tctaaattct ctccttttac aattcttttt aatgacctgg ctaactcttt aagtgttccc
     4141 ttacgaaact caattttaac caaccgtgat aaaatcgttt ccggaatttt ttcttcatcg
     4201 gtggtgcaca aaataaaaac gacatgggac ggcggctctt cgattacttt caaaagagca
     4261 ttgaacgcct ctttagtcaa catatgcacc tcatcaataa taaaaacttt cttttttaat
     4321 ttagacggca tcaaataaga actttccttt aaattcctga tatcgtcaat accgcggtta
     4381 gaagcagcgt caatttcaat cacgtctaaa ttagatccct tcaaaatagc caaacaattc
     4441 tcacattgat cacatggctc accattttcc ggagctaaac aattaactaa ccgggcgata
     4501 atccgagcca ctgaagtctt ccctgcccct tttggccctg agagcaaaaa tgattgaggc
     4561 gtttcctttc ccgaaaatag ctttttaagc ttttcaccta aattatccaa atccaactcc
     4621 cctaattttt gaggacgata ctgaagatga taaacagtca caattcatta tagcaagttc
     4681 gccaccccat gggaaagcca aaatgctaaa atatcagtta gagatttatg ctgaaacttt
     4741 gcctcatatt tcaacttttc agtacaaata acaatgtctc ctaatctaat ccaaccatca
     4801 gcattttttt ctttcgatat aggaaaactt aaaacttggg gaatataatt catctgccga
     4861 tactgctggt ttaattcctt agccttttgc cggccgacaa aaaccaaact taattcgact
     4921 cgatcccatc ccttcaaagt agcctgggct attcttctaa ctaaattctc agataatccc
     4981 caatttttag ggtgtttaat taaaatttta ttcacctgac aactgctctt taattatcct
     5041 ggctattaat tctcccgacg ctcttcctcc tgtttggttt ttaactgctc gcataatcgc
     5101 tccaaaatcg ttgcccagat tcgcccgcat ttcaccaacc aaagcggtta tttcttcaac
     5161 agtcatcatt tggggtaagt agctttttaa aagctcgatt tcctcatcca aggccgttac
     5221 taaatccaat cgcttaccct ttaaaaataa taaccgtgat tcttccttgt tttttaactc
     5281 tttttgaaga accattatct catctgtttc cgttaattgc cccatgggca aggttaattc
     5341 tttctgatct attagagaaa ttaaatacct tagcctttct acttttagat tatcgcctga
     5401 ctttagggcg gtgacactat cagcctttaa tttatctttg agcatatctt cgagattgtt
     5461 tggcacgccg acggcatttt tcccgccata aaataatctt cttcttagcg taacgccgct
     5521 ctgacggctt ccgataggcc gttcgttctt taatctcttc gctcacatcc tcctccaaag
     5581 ataaccgtct aaatctgcca ataagatcat ctttggtttc ccccttcttt cttactacaa
     5641 tcaatgccat atggagtcta ttttacctat tgtttggaat tttgtctaca gagaagttga
     5701 tccaccgcgt taattactcc atctttatta taatcaacct caacacaggc atctgtcgat
     5761 ttggtcggag ctacttttac cccctttaga caatcataat agtcaacact attaattacg
     5821 ccatcattat tataatcggt agtttgattg caactagctg aacttgaatc agttgccgtc
     5881 gggctaacga ctgggctgac caatggaaca gcctctactg tcggagttgg ctcaactatt
     5941 tcttttgtct gaaccgaatt agaaaaagaa taaggaatac cggcattatc gtaaacagta
     6001 tcattaatct taatcgtata gtaataaagg gtattatctt tgagcggact aagagtgatg
     6061 ttaaaatcgg ttccttcctc ggcggctgat ttgctcaaaa gcatcgttgt cggcccagtg
     6121 ccatacaaaa ctgtcggttg gcattttttc cctgtagtaa agctaatagt agccgactta
     6181 ccatcactac tgaccattgc tcgaacatct ttgggaccac tgtcagcggc tgccccagcc
     6241 atataagttc ttaccgcacc taaaccaact actaccaaaa cacccactaa aagtaaaata
     6301 acaattacta aaactgtttt aaccttaact gctcctttca taaagctttc aaaataaagg
     6361 cgctaattaa accaattccc cctaacagaa aaaagccaat tgtcgctgcc ggattacctg
     6421 ttttaggcaa ttcgggggtg cttgtcgttt ccgccggagt tgattgagtt ggagtattag
     6481 taggcgaact gctattgccg cttactgtaa tctcgtaaga accggcctga ttatcagcac
     6541 aggaaataac atcaactacc gttcctgttt cagtagttaa taaatttgaa tcggttaagc
     6601 ttgaaggagt acaagaaaaa gtcgctttag ccgttcctgt ttcctttccc ttaaatttaa
     6661 tagtaaacaa atccccggct acggcagttg attccaaacc atctgtagct ttatctttta
     6721 ctaaagtaac tgttatcttt tcaccctcaa ctttaggata gatg
//
LOCUS       DAOOIE010000012         6176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_10409_length_6176_cov_3.156347, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000012 DAOOIE010000000
VERSION     DAOOIE010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10409_length_6176_cov_3.156347"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..964)
                     /locus_tag="PK131_00750"
     CDS             complement(<1..964)
                     /locus_tag="PK131_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44693.1"
                     /translation="MERGLPVALVIGSDRLGGRKLIEKLRQQEVIVKDGAENYDDRVD
                     YIFDFEGERDIWRKAAENGTKVTIVAINDKKEAEAWENDIKSINGDLRLVKAFGVYGP
                     GMTGDSFMGKAFQSAVKNKNLILPSLSLKFRLLAEDDLVEAILRVNFLSGLERGVVLI
                     AGEEIDSQQVAAVLIDEAKMTRNQVIQSEIQVESFSDKEVRENWARLHWQPQIEFRDG
                     VKETLQYFFTQADEESRSSVINKSQRHRPTTPVKLTVEVEPSSFSTNESEEKEEENEE
                     KESKTTETNEPKFEIKPIIKKTEVERDYPEEEIEELDEGPVAIKP"
     gene            complement(997..2226)
                     /locus_tag="PK131_00755"
     CDS             complement(997..2226)
                     /locus_tag="PK131_00755"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HPD44694.1"
                     /translation="MLKKPIWNTLVQIIGKILTILISLITTAILTRKLGTGVYGAFIL
                     TTSVFLLLDALADFGSRAIGVREASKNPNRSEEIYQELTGLRLILANIGFVIGMIIIF
                     TFEGFAQIKLAALISLSMIFFTSLAGSLEIVFQTRMRLDLKTIMDVSFPLFFLILLLI
                     YPLKLTLVGVMVMYLVSRIVSLIIGQKLTHRPAWIVKFKQIGTWLKELWPMGVYLLVF
                     TAYDRAVDSMMISHFLSMNEVAWYGLSYKIYGNLVQPAYFFVASIFPLLSSPIPGKRK
                     LFFSSLSLIIIGLAITAPLLSWLAPWIINILGGIEFGPAIAVLRILIFAMIFAYLNHL
                     LGFTLIARGSQKSILFVGTVVLVVNIIGNWLVIPRWGINGAAGVTVLTEALSMILLGG
                     QLLRQRDNLRIITDKLP"
     gene            complement(2244..3296)
                     /locus_tag="PK131_00760"
     CDS             complement(2244..3296)
                     /locus_tag="PK131_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44695.1"
                     /translation="MLFLFLCCLPTQLGKHFWPAWSLIKGVRIDYLSPTIYFVDILAA
                     GLILPRFFKSLKSVNLVFWPLAVLNILIAGNKWLALTGWLRILEIYLVYLTLKPEKEL
                     IRKYLGYIIPFWIILEGGLGLAQVINGGSLQGVFYWLGERRFNLNTIGIAKISFYGRE
                     ILRAYGTFSHPNSLAGFLLITLELWRYYSSGWFYWLIRGLGLVTIIFSASRAAWLLLI
                     INSPFRIYGLLFLALFGVFSGWDPLSWTKRLTLNNEAVKLFLKHPLLGIGWNNYLSQS
                     HFYQPVHNIYLLILSQLGLIPTLILGSAIRSKIAPQKWPKRIWLIILITSLFDHYWLT
                     LPQNWWLLTLCFILYF"
     gene            complement(3366..3830)
                     /locus_tag="PK131_00765"
     CDS             complement(3366..3830)
                     /locus_tag="PK131_00765"
                     /inference="COORDINATES: protein motif:HMM:NF013583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HPD44696.1"
                     /translation="MDNKKRNKIILDLTCELLNKMNFEINKAAVEDSNDEGVLVSVEV
                     NNPAALIGFKGKNLAAVQLILTLIIKNKLGNWVKIILDVNQYRKEQKARLEAMTISLA
                     EKVLATGKPVTMANMSSYERRICHLVVAGRKGIITESEGEGEARHIVIKPET"
     gene            complement(3823..4608)
                     /locus_tag="PK131_00770"
     CDS             complement(3823..4608)
                     /locus_tag="PK131_00770"
                     /inference="COORDINATES: protein motif:HMM:NF014186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HPD44697.1"
                     /translation="MNILLAPFINALIGFYHLLGNFGWAVIIVTILIRLLLLPLVLPT
                     LKASKKMMLIQPKLKKIQDQFKDNKQALATAQMELYKKEGINPLAGCLPQILQIVILV
                     VFFMAFNMIVNFSMGQGDLVTLNKNLIPAFQLSEKTQVNLNFFGSDLAKTPKMIAQNG
                     WNFNMLIPLILLIGSAVFQYLSSKLMMPTAAKKTPVDQSAYVKATPDKSDDMMEAMRT
                     QSLIMMPLMTIILGWNFNLGILLYWFINSLVMLVQQVIIEKNG"
     gene            complement(4613..4819)
                     /gene="yidD"
                     /locus_tag="PK131_00775"
     CDS             complement(4613..4819)
                     /gene="yidD"
                     /locus_tag="PK131_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006805375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPD44698.1"
                     /translation="MKKVILGILNFYSRIPYWGHAGCRFIPSCSEYTKEAVEKYGAVG
                     GLILGLKRISKCHPFKKGGIDLVK"
     gene            complement(4782..5111)
                     /locus_tag="PK131_00780"
     CDS             complement(4782..5111)
                     /locus_tag="PK131_00780"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_function: GO:0042781 - 3'-tRNA processing
                     endoribonuclease activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HPD44699.1"
                     /translation="MLKKINRITSRKEFEIIKKEGKLKQYPYFGLIFKQIPISDNREI
                     NNKKFGFIISKRISKKAVIRNKIKRLIAEEVRQNLDKFENGFRGIFLVKRNILDEKGN
                     LRNLKFL"
     gene            complement(5113..5253)
                     /gene="rpmH"
                     /locus_tag="PK131_00785"
     CDS             complement(5113..5253)
                     /gene="rpmH"
                     /locus_tag="PK131_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008472873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPD44700.1"
                     /translation="MSTKRTYQPKKKKRVRVHGFLKRMATTSGRNVLKRRRSKGRTKL
                     TV"
     gene            5410..>6176
                     /locus_tag="PK131_00790"
     CDS             5410..>6176
                     /locus_tag="PK131_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012529.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HPD44701.1"
                     /translation="MDIEKLWKNTQEELKIVMAPAVYQTFMAKTQLVVYQNNHAVIAC
                     PNTYLADINLKRYYATFKNALDNQSQSNNTLEFIIQDPISNSPLTSPPPPLLEYSAPK
                     FNPNTGLHPRYTMENFIVGNSNNFAYAAAQAIIQNPGTAYNPFFVWGGVGVGKTHLIQ
                     AIGHELFKKYPSLKIKYFPAETFGNELVAALKAKNINPFKEKYRHLDCILVDDVQFLA
                     GKEYIQEEFFHTFNTLHMNGKQVVLTSDRKPSEIGHL"
BASE COUNT         2087 a         1301 c          868 g         1920 t
ORIGIN      
        1 ccggtttaat cgccactggc ccctcatcca attcctctat ttcctcctct ggataatccc
       61 gctctacttc tgttttcttt atgatcggct taatttcaaa ttttggttca ttagtctccg
      121 tcgttttact ttctttttcc tcattttctt cttccttttc ttcagattca ttagtagaaa
      181 aagatgatgg ctccacttct actgttaatt ttaccggtgt ggtcggccga tgacgttgac
      241 ttttattgat aaccgatgat cgactttctt catcagcttg ggtaaaaaag tattgtaggg
      301 tttctttaac cccatcgcga aactctattt ggggttgcca atggagacga gcccaattct
      361 ccctcacttc cttatctgaa aaactttcta cttgaatctc actctgaatc acctggtttc
      421 ttgtcatttt cgcttcatcg atcaggacag ctgctacttg ttgagaatca atttcctctc
      481 cggctatcaa cactactcct ctttccaatc cagaaagaaa attaaccctc aaaatggctt
      541 caaccagatc gtcttcggcc aagaggcgaa attttaagct gagtgacggc agaattaaat
      601 ttttattttt aactgccgac tgaaaggctt tgcccataaa actatcccca gtcattcccg
      661 gcccataaac accaaaggct ttaactaacc ttaaatcacc attaatagat ttgatatcgt
      721 tttcccaagc ctcagcctct tttttatcat taatggcaac tatagttact tttgtcccat
      781 tttcagccgc tttccgccaa atatctcttt caccttcaaa gtcaaaaata taatcaactc
      841 gatcatcgta attctcggct ccgtctttaa caataacttc ctgttgtctt aatttttcta
      901 ttaatttccg ccctcctaaa cgatcactgc cgataactaa agctactggt aatccccttt
      961 ccatacttct attatatcta ggatctttta tctagcctac ggtaatttgt cggttattat
     1021 gcggagatta tctctttgcc gtaaaagctg accacccagt aaaatcattg atagggcttc
     1081 agtcaaaaca gttacaccag ccgctccgtt aataccccaa cggggaataa ccaaccaatt
     1141 gccaattata ttaacgacca aaacaactgt acctacaaat aaaatcgact tttgactacc
     1201 cctggcaatc aaagtaaaac ctaaaagatg atttaaataa gcaaaaatca ttgcaaaaat
     1261 taaaatcctg agaacggcaa tagccggccc aaattctatt ccacctaaaa tattaattat
     1321 ccagggagct aaccaagaaa gtaaaggggc ggtaatcgcc aaaccaataa ttattaagct
     1381 taaagaacta aaaaacaatt ttcttttccc cggaattgga gatgacaata aaggaaaaat
     1441 actagctaca aaaaagtaag ctggttgaac caaattacca tagattttat atgacaaacc
     1501 ataccaagcc acttcgttca tacttaaaaa atgagagatc atcatcgaat caaccgcccg
     1561 atcataggcg gtaaaaacca gtaaataaac ccccatgggc cataattcct tcaaccaagt
     1621 accaatttgt ttgaatttca ctatccaggc tggccgatgg gttaattttt ggccgataat
     1681 caaactaacg attctcgaca ccaaatacat aaccattact ccaactaaag taagttttaa
     1741 gggatagata agaagcagaa ttaaaaaaaa caaaggaaaa ctaacgtcca taatagtctt
     1801 taaatccaac ctcattctag tttgaaaaac gatctccaaa ctcccagcta gactagtgaa
     1861 aaaaatcatt gatagggaaa ttagggccgc taatttaatt tgagcaaaac cttcaaaggt
     1921 aaaaataatt atcatcccga tgacaaaacc aatattggct aaaatcaatc gcaagccggt
     1981 caattcctga taaatttctt ccgatctgtt tggattttta ctcgcttccc taaccccaat
     2041 agcccgacta ccaaaatcag ccaaagcatc taaaagtaaa aaaacagagg tggtcaaaat
     2101 aaaagcacca taaacacctg ttcctaattt tctggttaaa atggctgtgg ttattaaact
     2161 aattaaaatt gttaaaattt tacctattat ttgaacaaga gtattccaaa tcggcttttt
     2221 caacataact tattttaccc tggttaaaaa tataaaataa aacacaaggt taataaccac
     2281 caattttgag gtaaagtaag ccaataatga tcaaaaagtg aggtaatcaa aattatcaac
     2341 caaattctct taggccactt ttggggggct attttagacc ttattgctga tcctaaaatt
     2401 agcgttggta ttaaacctaa ctggcttaaa atcaacaaat aaatattatg aactggctga
     2461 tagaagtgac tttggcttaa ataattattc caaccgattc ctaaaagagg atgttttaaa
     2521 aacaatttaa cggcttcatt attcaaggtc aatctcttag tccaacttaa cggatcccag
     2581 ccggaaaaaa ccccaaacaa agctaaaaac aacaaaccat aaatcctaaa tggactattg
     2641 ataattaaca acaaccaagc agcgcggctg gctgaaaaaa taattgttac caaacccaaa
     2701 cctctaatta accaataaaa ccaaccagat gaataatagc gccataactc aagggttatt
     2761 aataaaaacc cggctaaact attaggatga gagaaggtac cataggctct taaaatttcc
     2821 ctaccgtaaa aactaatttt agcaatacca atagtattta aattaaaccg tctttctccc
     2881 agccaataaa aaactccctg caaactacca ccattaatta cttgggctaa acccaaacca
     2941 ccctctaaga taatccaaaa aggaataata taacccaaat acttcctgat taattccttc
     3001 tcaggtttta atgttaaata aactaggtaa atttctagaa ttctcaacca tccggtcaag
     3061 gctagccact tattaccggc gattaaaata tttaaaacag ccaggggcca aaaaaccaaa
     3121 ttgacagatt taagtgattt aaaaaatctg ggtaaaatta atcctgccgc taaaatatca
     3181 acaaaataga ttgtcggtga taaatagtca atcctaacac ctttaattaa actccaggct
     3241 ggccaaaaat gtttgcctaa ttgtgttggt aaacaacata aaaaaagaaa aagcaaacaa
     3301 tttttaagcc gagttggtaa aaaagaggac aattaaaaat aattaaaaat taaaaatttt
     3361 tattattaag tttctggctt aatgacaata tgtcttgcct ctccctcacc ttctgattca
     3421 gtgatgattc cttttctacc agccacaacc aaatgacaaa ttcgccgctc ataagaactc
     3481 atattagcca ttgttaccgg cttaccagtt gctaaaactt tctcggccaa actaattgtc
     3541 attgcttcca aacgcgcttt ttgttctttc cgatattgat tcacatctaa aataattttt
     3601 acccaattac ccaatttatt tttaattatt aacgttaaaa tcagctgaac cgccgctaag
     3661 ttttttcctt taaaaccaat cagggcagcc ggattattca cttcgaccga caccaaaact
     3721 ccctcgtcgt tactgtcctc aacggccgct ttattaattt caaaattcat cttattgagc
     3781 agttcacagg ttaaatctaa aataatcttg tttctttttt tattatccat ttttttcaat
     3841 aatcacttgt tgaacaagca ttactaaact gtttataaac caataaagga gaatacccaa
     3901 attaaagttc cagcctaaaa taatagtcat taacggcatc ataataagtg attgggttct
     3961 cattgcctcc atcatatcat cacttttatc cggggtcgct ttaacatagg ccgactgatc
     4021 gactggggtt tttttagcag ctgtcggcat cattaattta gaacttaaat actgaaacac
     4081 ggccgatcct atcaataaaa ttaatggtat taacatgtta aagttccagc cattttgggc
     4141 aatcatttta ggtgttttag ctaaatcact accaaaaaag ttcaaattaa cttgagtctt
     4201 ttctgataat tgaaaggcgg gaattaaatt cttattaaga gtcactagat ccccttgacc
     4261 catggaaaaa ttaactatca tattaaaggc cataaaaaac actaccaaaa taacaatctg
     4321 caaaatttgg ggtaaacagc ctgccaacgg attaattccc tctttcttgt ataattccat
     4381 ttgagccgtg gctaaagctt gcttattgtc cttaaattga tcttgaattt tttttaattt
     4441 aggctgaatt aacatcattt tttttgaggc ttttagggtt ggtaaaacca aaggtagaag
     4501 taaaagtcga attaagattg tcacaataat tactgcccag ccgaaatttc ccagaagatg
     4561 gtaaaaacca attagagcat taataaaagg ggcgagtaaa atattcatag gtctatttta
     4621 ccagatcaat accccctttc ttaaagggat gacatttact aattcgcttt aaacctaaaa
     4681 ttaaaccacc aaccgcccca tatttttcta cggcttcttt agtatattca ctgcaactgg
     4741 gaataaatcg acaaccagcg tgcccccaat aaggaatccg gctataaaaa tttaagattc
     4801 ctaagattac ctttttcatc aagaatattc cttttaacta aaaaaatacc acgaaaacca
     4861 ttttcaaact tatcaagatt ttgtctaacc tcttcagcaa tcaatcgttt aattttattt
     4921 cttatcactg cttttttaga aattcgtttc gaaataataa aaccaaattt tttattattt
     4981 atctcccggt tatcactgat tggaatttgt ttaaaaatta aaccaaaata agggtattgt
     5041 tttaattttc cttccttttt aattatctcg aattccttcc gactagtaat tcgattaatc
     5101 ttcttgagca ttttaaactg ttaatttcgt tcgcccctta gatcttcttc gttttaaaac
     5161 atttctacct gaggtggtag ccatcctttt taaaaaacca tggaccctaa ctcttttttt
     5221 ctttttaggt tggtaagttc ttttagtcga catatccaat attaccatat tacccctctt
     5281 tatccaccgg taagttaaca ggtcgaagag ggagttagaa taaaattaat ttgatccctt
     5341 ttaaattgtg gaaaactcat ttatgataga tctgattgaa ccccccagaa atcatctttt
     5401 tagtttaata tggacatcga aaaactttgg aagaatacac aggaagagtt gaaaatagtc
     5461 atggcgccgg cagtttacca aactttcatg gccaaaaccc aattagttgt ttaccaaaac
     5521 aaccatgccg ttattgcctg tcccaatacc tacttggctg atattaattt aaaacgctat
     5581 tatgctactt ttaaaaacgc actagataat caaagtcaaa gtaataatac tttagagttt
     5641 attatccaag atccaatctc caactctcct ttaaccagtc caccccctcc tttattggaa
     5701 tactctgctc ctaaatttaa tcccaatacc ggcctccacc cacgttatac tatggaaaat
     5761 tttattgtcg gtaatagtaa taattttgcc tatgcggcgg ctcaagccat tattcaaaat
     5821 cctggcacag cttataatcc attctttgtc tggggtggag taggtgttgg taaaacacat
     5881 ctaattcagg ccattggcca tgaattattt aaaaaatatc catcattaaa gataaaatat
     5941 tttccggccg aaacttttgg caatgaactc gttgctgccc tgaaagctaa aaatatcaac
     6001 ccatttaaag aaaaatatcg tcacttagat tgtattttag ttgacgatgt tcaatttttg
     6061 gctggtaaag aatatattca ggaagaattt ttccacacct ttaacactct ccatatgaat
     6121 ggaaaacaag tggttttaac ttcagatcgg aaaccaagtg aaattggcca tttaga
//
LOCUS       DAOOIE010000013         6146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_10463_length_6146_cov_3.675915, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000013 DAOOIE010000000
VERSION     DAOOIE010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10463_length_6146_cov_3.675915"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /gene="thrS"
                     /locus_tag="PK131_00795"
     CDS             <1..1126
                     /gene="thrS"
                     /locus_tag="PK131_00795"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPD44702.1"
                     /translation="RTLNNYVESLQSAKGYTQVWTPQIAKAKLFKISGHLDKYKDNMF
                     LVTSNYGREKFYLKPMNCPQHTQIYASQSRSYRDLPLRYTDFAMLYRDEKPGEISGLA
                     RTRSFSQDDCHVFCREDQVDAEIDTMLAMIKEIMATFGFKYKYRLSTRDSKHSKNFLG
                     SSQTWDKLEKWSREILKRNQIDYFEGPGEASFYAPKMDLIATDALNRDWQLSTIQIDY
                     VMPERFNLEYTDINGQKKRPVMIHRAMIGSPERFMMILLEHFGGNFPVWLAPVQVKII
                     PITDQQLNYANKIGIILKDNQIRSEVDDRSETMQNKIRRSSGQKIPYQLIVGSREADS
                     SNNLVAVRQRDGQDLKNMPLDDFLAAVKKQVEQRSLNLIK"
     gene            1131..1655
                     /gene="infC"
                     /locus_tag="PK131_00800"
     CDS             1131..1655
                     /gene="infC"
                     /locus_tag="PK131_00800"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPD44703.1"
                     /translation="MHQYYRINQQITAPTVRLIDESAKQIGVMSLAEARQHSFETGLD
                     LVEIAPKSVPPVVKLINFNKFKYQEAKKAKAGKKGSRGGELKEIQMTPFIGQKDYEDR
                     VKRAKKFLATGNKVKLNIKFQGRQITHQEFGHQLIDKFKADLADIAAPEGEAKLLGKK
                     LFSVFYGKKTKIKN"
     gene            1627..1830
                     /locus_tag="PK131_00805"
     CDS             1627..1830
                     /locus_tag="PK131_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003970213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bL35 family ribosomal protein"
                     /protein_id="HPD44704.1"
                     /translation="MGKKQKLKTKKSISSRFEVTKNGRVLRTASFRRHLRRHKSKKQL
                     RRLKGKKRVTGRMAVKIRKLLGK"
     gene            1853..2194
                     /gene="rplT"
                     /locus_tag="PK131_00810"
     CDS             1853..2194
                     /gene="rplT"
                     /locus_tag="PK131_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPD44705.1"
                     /translation="MRVKTGFTRRQHHKKVLKKAKGFWMTRHKRYKAAHEAVMHAGRY
                     TFIGRRLRRRDMRQLWIVRLNAAIRPFGLSYSQFIKKLKNNKIELNRKMLSEIAIRDH
                     QTFKTIVDSLK"
     gene            2198..3160
                     /locus_tag="PK131_00815"
     CDS             2198..3160
                     /locus_tag="PK131_00815"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF013568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPD44706.1"
                     /translation="MEDYLKKIAECTEADELEKLKIKLLGRRGVLNRLLADVKNLPVE
                     KRIELNKLKKEIETAFANKKIETPASRRTSLLKFSSAKLGHLHPLTQTENQLNNLFRS
                     LGFSIYDGPEIETDEFCFERLNVPQNHPARDLQDTIYLQEPEILLRTQTSSIEARLLY
                     EQKPPFKVVAPGCCYRNEKVNKSNHFILHQYQGVVVDKNITLKDLFGTLNLLFKTLYG
                     PEVIIRFRSKYYPEVEPGVGPDMQCFACHGVGCPLCKGAGWIEMGGAGIIHPKVLKMA
                     NIDPCQWTGFAFGLGLDRWTMAKFNLKDIRTLLGGNLAYKPNEL"
     gene            3157..4857
                     /locus_tag="PK131_00820"
     CDS             3157..4857
                     /locus_tag="PK131_00820"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HPD44707.1"
                     /translation="MKIIYSQLKQFLPDLKHSAKKIAEDLTWIGHFCDGFEQKDDEEI
                     ISLEIRSNRGDCLGYLGLARELAILYNLDLKIPQYSLKPTSSPLNIKVDSLDVKRVMA
                     IKIRDLHLAESSSEIKKFLKLHQIGLNNNIVDLTNYIMLLYGIPCHAFSLSNDLIWEN
                     LTTSTTFTTLDGSKINLPPTTLVISHQQQPTCLSFIGGQNSGIQLNTKEAIIEMAVYN
                     PTRVRLDSKKLNLTTEASLRLDKELDPDLIPTAFNHLIKLILNHSKGHIDSALYDYYP
                     NKTPHKNISLTINPSAIAGIKIPDEFSQKIINKIDIDHRSDIESAIDLVEEIIRFWGF
                     NKIPTDQPVNPRILPDITPQILKNIETLKSELLSRGFNEVKTKPLTNKKYSKSIATQN
                     NINANLPYLRTNLTDSLEEQLNQYLRLKIPLQPFFEIGKIYYQENNNLVEKYALGTFD
                     GKFHETIIDDTPREFKKLPDTFFNAYEITSQIICLDANLISSKTEKELLAYYRKAIGN
                     ILWDIKIIDHYRNKYTFRVWYFNCDDKTAKQIHLKTFKLTKIQRTLSKKRKNYTKDKI
                     "
     gene            complement(4927..5052)
                     /locus_tag="PK131_00825"
     CDS             complement(4927..5052)
                     /locus_tag="PK131_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44708.1"
                     /translation="MVFTNVRLNLNHPMHEEIILQIVIKNNPSLAISKNLSYIDR"
     gene            complement(5287..>6146)
                     /locus_tag="PK131_00830"
     CDS             complement(5287..>6146)
                     /locus_tag="PK131_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44709.1"
                     /translation="CLGWTPNLLVAVWVGNNDNTPMNRVASGISGATPIWRRIMLTAL
                     PRRPKEDFPIPDKIITMDVDRLSGYPAHDGFPAKTAYFIDGTQPKTSDPIHLKLKVCK
                     GISGLATPEDIANGNYEEKEYIKLKEDCPGCQAGIDSWILDQPDREKYFPPEDYCRSN
                     GLITVDFETPGDQTTVGTDFEVKVKTNSVKKVTEVKLWVNGIEKYNWTEKPFETRLNL
                     NDGVYTLKIEAKDKDNNSASREIKIGVNMPWTGPLPTLTPTLTPTLTPTLTPAPIITP
                     TITVIPSGT"
BASE COUNT         2053 a         1219 c         1083 g         1791 t
ORIGIN      
        1 ccgaactctt aacaattatg ttgaatcact tcagtcggcc aaaggctata ctcaggtttg
       61 gacccctcaa attgccaagg ctaaactatt taaaatctcc ggtcatttgg acaaatataa
      121 agataatatg tttttagtta cctctaatta cggtcgggaa aaattttatc ttaaaccaat
      181 gaattgccca cagcacactc aaatttatgc cagccaatca agaagctaca gggatttacc
      241 cttaagatac actgattttg ccatgcttta tcgggatgag aaacccggag aaatcagcgg
      301 cttagcccga acccgttctt tctcccaaga tgattgtcat gttttctgcc gagaagatca
      361 ggtcgatgcc gaaatcgaca ctatgctggc gatgattaaa gaaattatgg ccacttttgg
      421 atttaaatat aaatatcgcc tatcaacccg tgattctaaa cactcaaaaa attttttggg
      481 gagtagtcaa acttgggata aattagaaaa atggagccgg gaaattttaa aacgaaatca
      541 aattgattat tttgaaggtc cgggtgaagc tagtttttat gcgccaaaaa tggatttaat
      601 agccactgat gctttaaatc gggattggca gttatccacc attcaaattg attatgttat
      661 gcctgaaaga tttaatttag aatataccga tattaacggt cagaaaaaac ggccggttat
      721 gattcaccgg gccatgattg gttcaccaga aagatttatg atgattttat tggagcattt
      781 cggcggcaat tttccagtct ggctggcacc agtccaggta aaaattattc ctatcactga
      841 tcaacagcta aattatgcca ataagattgg tataatttta aaagacaacc aaatccggtc
      901 tgaagttgat gataggtcgg aaacgatgca aaataaaatt cgccgctcca gcggccaaaa
      961 aatcccttac caattaatag ttggttcccg ggaagcagat agttccaata atctggtagc
     1021 tgttcgccag cgggatggcc aagatttaaa aaacatgcct ctagatgact ttttagcggc
     1081 cgttaaaaag caagtcgagc aacgcagttt aaatctgata aaataaaaaa atgcatcaat
     1141 attaccggat taaccaacaa attactgctc cgactgttcg tttgattgat gaaagtgcaa
     1201 aacaaatcgg agttatgtcg ttggctgaag cccgccaaca ctcttttgaa accggtctcg
     1261 atttagtcga aatcgccccc aagtctgtcc caccagtagt caaactaatt aactttaata
     1321 aatttaaata tcaggaagcc aaaaaagcca aggcggggaa aaaaggcagt cggggagggg
     1381 agctaaaaga aattcaaatg acgccattta ttggccaaaa ggattatgaa gatcgggtca
     1441 agcgagctaa gaaattttta gctactggaa ataaagtcaa attaaatata aaattccaag
     1501 ggcggcagat aacccatcag gaattcggtc atcaattaat tgataaattt aaagccgatt
     1561 tggccgatat tgcggctcct gaaggggaag ccaaactttt aggtaaaaaa ttgtttagtg
     1621 tattttatgg gaaaaaaaca aaaattaaaa actaaaaaat caattagcag tcgttttgag
     1681 gtgactaaaa atggccgggt tttacgaacg gccagttttc gccgtcatct tcgccggcat
     1741 aaatctaaaa agcaattacg acgattgaag ggtaaaaaac gagttaccgg ccggatggct
     1801 gttaaaatta gaaaattgtt aggaaaataa ttagtaatta aaaaataaaa ctatgcgagt
     1861 aaagactggt tttaccagac gtcaacacca taaaaaggtt ttgaaaaaag ctaaaggttt
     1921 ttggatgacc cgccataagc gttataaggc ggcccatgag gcagtaatgc acgccggccg
     1981 ttataccttt atcggccgaa ggctgcgccg aagagatatg agacaactgt ggattgtcag
     2041 attaaatgcg gcaattaggc cctttggtct ctcttactct caattcatta aaaaacttaa
     2101 aaataataaa attgagctta accgaaaaat gctttcagaa attgccattc gtgatcacca
     2161 aacttttaaa accattgtcg attcgttaaa ataaagagtg gaagattact taaaaaagat
     2221 tgccgaatgt accgaggcag acgaactaga gaaactaaaa attaaattgc taggccgtcg
     2281 cggagttctt aatcgacttt tggctgatgt caaaaatctt ccggtagaaa aacgcatcga
     2341 gttaaataaa ctcaaaaaag aaattgaaac tgctttcgct aataaaaaaa tagagacgcc
     2401 tgccagccgg cggacatctc tattaaaatt ttcttcagcc aaacttggcc atctgcatcc
     2461 tctaactcaa actgaaaatc agctcaataa tttattccga tcgttaggct tttctatcta
     2521 tgacggtccg gagattgaaa ccgatgaatt ctgtttcgaa agactcaatg ttccccaaaa
     2581 tcatcctgcc cgtgatcttc aagatactat ttatcttcaa gaaccagaaa ttttacttag
     2641 aacccaaact tcctcaatcg aagctagatt actttacgaa caaaaaccac cctttaaggt
     2701 ggtagcaccg ggttgctgtt accgcaacga aaaggtcaac aaatcaaatc attttattct
     2761 tcaccaatat caaggggtag tagtcgataa aaacattact cttaaagacc tattcggtac
     2821 tttgaattta ctgtttaaaa ctctttatgg tcctgaagta attattagat ttagaagtaa
     2881 gtattatcct gaagttgaac caggcgttgg tcctgacatg cagtgtttcg cttgccatgg
     2941 tgttggttgc cctctctgta agggtgctgg ctggattgaa atgggcgggg ccggaataat
     3001 tcaccctaaa gtcctaaaga tggcaaatat tgatccttgt caatggactg gtttcgcttt
     3061 tggtttgggc ctcgatcgtt ggaccatggc taaatttaat cttaaagaca tccgcactct
     3121 tctaggcggc aatctcgcct acaaacccaa tgaattatga aaattattta ttctcaactt
     3181 aaacagtttt tacccgattt aaagcatagc gctaaaaaaa ttgccgagga tttaacctgg
     3241 atcggtcatt tttgcgatgg ttttgaacaa aaagatgatg aagaaattat ttctttagaa
     3301 atccgctcca atcggggaga ttgccttggt tacttaggtt tagcccgcga actagccatt
     3361 ctttacaacc tcgacctcaa aatccctcaa tatagtctaa aacccacttc ttcaccctta
     3421 aacattaaag tcgattctct cgatgtcaaa agggtaatgg ctattaaaat cagagattta
     3481 cacctcgcag aatcctcatc tgaaattaaa aagttcctaa aactccacca aataggcctc
     3541 aataataaca tagttgattt aaccaattat attatgcttt tatatggcat tccctgccat
     3601 gctttttctt taagtaatga cttaatttgg gaaaacttaa ctacctcaac tactttcacc
     3661 actcttgacg gcagtaaaat caacctcccg ccaaccactt tagttatcag tcatcagcag
     3721 cagccaacct gcctttcatt tattggaggt caaaactctg gaattcaact taacactaaa
     3781 gaagcaatta ttgaaatggc agtttacaac ccaaccagag ttcgccttga ctctaaaaaa
     3841 ctaaatttga ctaccgaagc tagtctccgc ttagataaag aacttgatcc cgatttaatt
     3901 ccaactgctt ttaatcacct gattaagtta attttaaacc attctaaagg tcatattgac
     3961 agtgctttat atgattatta tccaaataaa actccccata agaacatatc gctaactatc
     4021 aatccatctg ctattgccgg catcaaaatc cctgatgaat tttcccaaaa aattattaat
     4081 aaaatcgata ttgatcatcg gtccgatatt gagtcggcca ttgatttagt tgaggagatt
     4141 attcgttttt ggggttttaa taaaattcct accgatcagc cagttaatcc tagaatattg
     4201 cccgacatta ctcctcaaat tttaaaaaac atcgaaactc ttaaatcaga attactcagc
     4261 cgcggtttca acgaagttaa aaccaaacca ttaactaata agaaatattc caagtcgatt
     4321 gccactcaaa ataatattaa tgctaatctg ccatatttac gaacaaattt aactgattct
     4381 cttgaggaac aattaaatca gtatcttcgc ttaaaaattc ctctgcaacc cttttttgaa
     4441 atcggcaaaa tttattacca ggaaaataat aatttagtcg aaaaatacgc tcttggaacc
     4501 tttgacggta aatttcacga aacaatcata gatgatactc ccagagaatt taaaaaacta
     4561 cctgatacct tttttaatgc ttacgaaatt acctcccaaa taatctgcct tgatgctaat
     4621 ttaatttctt ccaaaacaga aaaggaatta ctggcatatt atcgaaaggc aattggtaat
     4681 attctttggg atattaaaat tattgatcat taccgcaata aatatacctt cagggtttgg
     4741 tatttcaatt gcgacgacaa aactgccaaa caaatccact taaaaacatt taaattaaca
     4801 aaaatacaga gaacattgag taaaaagaga aagaattaca caaaagataa gatatagtat
     4861 catgcaacat ccctacatta acaactattg aaacattata tgatttaacc atggataaca
     4921 cccgtttcac cgatcgatat aggataaatt cttcgaaatt gccaggctgg gattattctt
     4981 tatcactatt tgaagaataa tttcttcgtg catgggatga ttaagattca gcctaacatt
     5041 ggtaaaaacc atgtagagat tttaaactgt tgaataataa atctaatcaa acaatcccta
     5101 aaattatttg tacttacaag gcatcagtaa aaaggtatgc ttggaagaat aaaatacttt
     5161 ttggttggca agcaagatat ttagacaagg tcatttttga tgaaaagaga tactatcaaa
     5221 aattacataa aaaataatct taagaattgg gtgaaagacg attggtttaa tgagaaatag
     5281 tcctttttat gttccactcg gtattactgt tattgttgga gtaattatcg gcgctggtgt
     5341 caacgtcggg gtaagtgtcg gggtaagtgt cggggtaagt gtcggtagcg gcccggtcca
     5401 aggcatatta actccaatct taatttctcg actggcactg ttattatcct tatctttagc
     5461 ctcaattttt aaggtataaa ccccatcatt taaatttaaa cgggtttcaa acggtttttc
     5521 cgtccagtta tatttttcta tcccattaac ccataatttg acttccgtca cctttttaac
     5581 cgagtttgtt ttgaccttaa cctcaaaatc tgttcctact gttgtctgat caccaggcgt
     5641 ctcaaaatca acagtaatta acccgttgct tcggcaataa tcttctggcg gaaaatattt
     5701 ctcccgatct ggttgatcta aaatccaact atctattcct gcctgacaac cgggacaatc
     5761 ctcctttaat tttatatact ctttctcttc ataattacca ttagcaatat cctccggcgt
     5821 agccaaacca ctaatccctt tacaaacttt taattttaaa tggattggat cggaagtttt
     5881 tggttgagta ccatcaataa aataggccgt tttggccgga aaaccatcgt gggctgggta
     5941 accggagagt cgatcaacat ccatggtaat aatcttatcg ggaataggaa aatcttcttt
     6001 aggccgacga ggcaaggccg tcagcataat tcgccgccaa attggagtcg ctccactaat
     6061 tcccgaagcg actcgattca tcggcgtatt atcattattg cccacccaaa cagccaccaa
     6121 aagattcggg gtccagccaa gacacc
//
LOCUS       DAOOIE010000014         6068 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_10621_length_6068_cov_3.007151, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000014 DAOOIE010000000
VERSION     DAOOIE010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 6068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6068
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10621_length_6068_cov_3.007151"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /locus_tag="PK131_00835"
     CDS             <1..1172
                     /locus_tag="PK131_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPD44710.1"
                     /translation="NGVDLSKDPQALQRVREAAEKAKIELSSAVETDINLPFITSKDG
                     QPLHLTKKLTRAELEKLVDDLIQKTIGPVEKCLKDAKVSKSDISEVIMVGGMTRMPKV
                     LETVKKFFGKEPNTTVNPDEVVAVGAAVQGAVLTGEVKDIVLLDVTPLTLGIETAGGV
                     RTVLIERNTTVPTKKSQIFTTYSDNQPQVEINVLQGERPMAADNKSLGRFILDGIAPA
                     PRGIPQIEVTFDIDSNGILSVSAKDKGTGKEQKITIQNATNLSKEEVEKMKGEAEAHA
                     REDEEKQLLAESRNRLDATIFSGEKTLKDFADKIKPDDKGKIEEKLKEAKELLGKAEV
                     NKDEFDRMSEELSQILQTVGQAVYGQQAGPTPTTEEAPKEEPDGDKKAEEGEIIN"
     gene            1231..2094
                     /locus_tag="PK131_00840"
     CDS             1231..2094
                     /locus_tag="PK131_00840"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HPD44711.1"
                     /translation="MKRDYYEVLGVSKSAGQSEIKSAYRKLALQYHPDRNKSPDAEEK
                     FKEINEAYEVLSDTEKRTAYDNYGHAAFDPSQGGFGGHTYTQENGPFHFTWTSSGQNS
                     FDNADFDFGGFSNPFDIFEQFFGNPVFSSGHSRSRSQLKTYKIQLSFLEAARGCEKEI
                     RIDGRTKTIKIPAGVDDGQQIRFSDFILYVDVLPDKIFKREGNDVYVIQDISLKQAIL
                     GDVIEVPTLDGYLKVRVQPGTQPNTLIRLKGKGIRDLRGGYQTGDFYIRLNVVIPTHL
                     TLHQKQILKEF"
     gene            complement(2166..2786)
                     /locus_tag="PK131_00845"
     CDS             complement(2166..2786)
                     /locus_tag="PK131_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44712.1"
                     /translation="MKRKIVISTILAGAMLLSSAGIVFGYAEPGPASAPVCNNEKPQK
                     AWLYRVKSLGNGKYQLFWDKADRATSWTIGYGVEPGNYIYGIHNFGDSQSRDLVVNTF
                     TNKKMYFAIRANNDCMPGEWSNEWKVGTGTGGVTAAAVKQTGATPTTQTPIVTKAPAK
                     AAEISPVPTTKKETVKETAPAAQPAPVVTPTPKKGGFWEWLKSLFR"
     gene            2850..3815
                     /locus_tag="PK131_00850"
     CDS             2850..3815
                     /locus_tag="PK131_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPD44713.1"
                     /translation="MNQKEKLLVVMIPCFNEEKTIGQVIDRIPRKIRGISKTKVLVIN
                     DGSSDCSAAIARKHGAIVISHPINRGLGQTFIDGIDKALRLGADIIVNIDGDLQFNPK
                     DIPKLVKPIIEEHFNFVTGTRYKDHREYNMKNRGLKNWGNKVFVRIINWITGHNYTDV
                     SCGFRAYSRKAALKLQLFGHFTYTHETFLDLVHKGFIACEVPVKVRPLRQNGQSHVSG
                     NLFKYAYSALKIIGRSFRDYHPFKFFGSISLIVFLVGLIPIVIFFVHYFTTGRMSPFK
                     SYAIGGAFLLVMSFLIFMLALLADMMGRIKDLEEEIIYQIKRKNK"
     gene            3885..4604
                     /locus_tag="PK131_00855"
     CDS             3885..4604
                     /locus_tag="PK131_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44714.1"
                     /translation="MNVGIIGFGQIGRAIKQVVEKKHRVFARDLDFDQIKKNRIDILH
                     LCYPFDKNFTINAVRYIKEIKPNLVIIESTVKVGTTEKIYRKTGVNLVHSPVRGVHPH
                     LYEGVMTFPKYIGSTSKKAGIIAKKYYQDLGIKVELFDDSRTTELAKLMCTTYYGWNI
                     IFEKEMYKLCHKYGVNFDQAYSRWNQSYNRGYRQLGMGYVVRPVLKHIPGPIGGHCVI
                     SNCGLITSSITRIISKFNKNY"
     gene            4622..5857
                     /locus_tag="PK131_00860"
     CDS             4622..5857
                     /locus_tag="PK131_00860"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HPD44715.1"
                     /translation="MPWRAFFFRNLTTKQTLIKNTFWLGLAEIVAKLVMFVVTILVVR
                     SFGPTNFGRLNLATAYAAIIVVFADFGLNIITTREIAKNKSEKDKYLSNIFTIKLIIS
                     LIIWLITVFWVRDRLILLVLTFSLLQNFCLLLGAVFSGLEAMQYNFISRSIYYLGLLA
                     AAVWVTRTNPNPERLMKSYILTTSLAFIVTFFMLKIKKITLRPAFDFSFWRKIFKETL
                     PFVGSSIIGVIYLNLDSILIGRYFGPEQVGFYQSAYKILFAFQSINIVNNSLFPRISA
                     LMAEEKYTTLKKLNVVVISLSLLGLIPLGVLMTILAKPIMRIIYGSIYVSAAPAMVCL
                     IWSGIIFYFRNYVNNLLVAANQQYRVFYSLLAGTTVNALFNLLITPKFNFIYASIAMV
                     LSELTILIYSSIQYARKEK"
     gene            5838..>6068
                     /locus_tag="PK131_00865"
     CDS             5838..>6068
                     /locus_tag="PK131_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008083412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44716.1"
                     /translation="MLEKKNNTYVIIGTKAQLVKMAPVMLELEKRKIDYTFISTGQHQ
                     ETIKDLISTFQIKKPDFYLYNGKDITKIWQMLI"
BASE COUNT         1717 a         1152 c         1268 g         1931 t
ORIGIN      
        1 agaatggagt tgatttatct aaggatcccc aagcgcttca gcgggtgcgg gaagcggcag
       61 aaaaggctaa aatcgagctt tcaagtgctg ttgaaaccga tattaacctg ccttttatta
      121 cttctaaaga cggccagcca ttgcatttaa ccaaaaaatt aactcgagcc gagttagaaa
      181 aattagtcga tgatttgatt cagaaaacta ttggtccggt agaaaaatgt ttaaaggatg
      241 ccaaagtgtc taaatcagac atcagtgaag tgattatggt cggcggcatg acccggatgc
      301 cgaaggtttt agaaaccgtt aaaaagtttt ttggcaaaga gcctaatact accgttaatc
      361 ctgatgaggt ggtagcagtt ggtgccgctg ttcaaggggc tgttcttacc ggcgaagtta
      421 aagacatcgt tttactggat gttaccccct tgactttggg gattgagacc gccggcgggg
      481 taagaacggt tttgattgag agaaatacta ccgttccgac caaaaaatcg cagattttta
      541 ctacctactc cgataatcaa ccccaggtgg aaattaatgt tctccagggt gagagaccaa
      601 tggcggctga taataaatcc ttaggccgct ttatcctcga tggcattgcc ccagcgccac
      661 ggggcatacc tcaaatcgag gttactttcg atatcgatag caatggtatt ttgagtgttt
      721 cggctaagga taagggcact ggtaaagagc aaaagattac tattcagaat gcgactaatc
      781 tgtctaaaga agaagtcgag aagatgaaag gggaggcgga agctcatgcc cgcgaggatg
      841 aagaaaagca gcttctagct gaaagccgta accggctcga tgcgactatt ttcagcggcg
      901 aaaagacctt gaaggatttt gctgataaaa ttaagcccga tgataagggt aaaattgaag
      961 agaagctgaa agaggctaaa gaacttttag gtaaagccga ggtcaataaa gatgagtttg
     1021 accggatgtc ggaggaatta agtcaaattc tgcagacagt cggtcaggca gtttatggtc
     1081 agcaggctgg acctacccct actacggaag aggctcccaa ggaagaacct gatggagata
     1141 agaaggccga ggagggggaa attattaatt aagtaagtaa aaaaggtgga atgacacccc
     1201 gccgcggcgg ggtgtcatta aataaattga atgaaacgcg attattacga agtcctcggg
     1261 gtcagtaaat cagccggtca gagtgaaatt aaatccgcct accggaaatt agctctccaa
     1321 tatcatccgg acaggaataa atctcctgac gccgaggaga aatttaaaga aatcaacgag
     1381 gcttatgagg ttttaagtga tacggaaaag cgcactgcct atgataatta cggtcacgcc
     1441 gcttttgatc ctagtcaagg cggcttcggc ggccatactt atacccagga aaatggacct
     1501 ttccatttta cttggaccag ttctggccaa aattcttttg ataatgctga ttttgatttt
     1561 ggcggttttt ccaatccctt cgatattttt gaacaatttt tcggcaatcc cgtttttagt
     1621 agcggccatt cccgttcccg atcacaatta aaaacttaca aaattcagct tagtttttta
     1681 gaagcggccc ggggttgtga aaaggaaatt cgaattgatg gccgaaccaa gacaataaaa
     1741 attcccgctg gagttgatga cggccagcag atcagattta gcgactttat tttatatgtt
     1801 gatgttttac ccgataaaat ttttaaacgt gaaggcaacg acgtttatgt tattcaggat
     1861 attagcttaa aacaggcgat tcttggtgat gtaattgaag tgccgacttt ggatggttac
     1921 ttaaaggttc gggttcaacc cgggacccag ccaaacactc taattcgtct taagggtaag
     1981 ggaattcgtg atcttcgcgg cggctatcag acaggtgatt tctatattcg tctcaacgta
     2041 gttattccta ctcaccttac tctccaccaa aaacaaatac tgaaagaatt ctgaaaattc
     2101 tgcacgcaga cactctctta gctgctttta attccttcct ttttaaaatc tcgatttaat
     2161 ttaacttacc tgaataagct tttcagccat tcccaaaaac cacctttctt aggggttggg
     2221 gtaacaactg gagctggctg agctgccgga gccgtttctt taaccgtttc tttcttagtt
     2281 gttggcaccg gactgatctc ggccgcttta gccggagcct tagttactat cggagtttga
     2341 gtggttggcg ttgctcctgt ctgcttcacc gccgctgctg tcactccccc cgtgccggta
     2401 cctactttcc attcatttga ccattcaccg ggcatacaat cgttattggc cctgatagca
     2461 aaatacattt ttttattagt aaaagtgttg acgactaaat ctcttgactg gctgtcgccg
     2521 aagttatgga tgccgtagat ataatttccc ggttcaacgc cgtagccgat agtccatgaa
     2581 gtggcccgat cggctttatc ccagaaaagc tggtatttgc cgttaccaag cgacttaacc
     2641 cgatacaacc acgctttttg tggtttttca ttattgcaaa ccggagcgct ggctggtccg
     2701 ggttcagcat agccaaaaac gatcccggcc gaacttaaca acatggctcc cgccaaaatg
     2761 gttgagatga caatttttct tttcataatt ccattattcc cctcttaaaa cttttgtcaa
     2821 gaagtaaatt tttttgctat actccgaaag tgaaccaaaa agaaaaactt ttagttgtaa
     2881 tgattccttg ttttaatgaa gagaaaacta ttggccaggt tattgatagg attccacgga
     2941 aaattagagg tatttctaaa acaaaagttt tggttattaa tgatggttcg agtgactgtt
     3001 cggccgctat cgcccgaaag catggggcaa tagttatttc tcatccgatt aatcggggat
     3061 taggccagac ctttattgat ggtattgata aggctcttcg tttaggggcg gatattattg
     3121 ttaatattga tggcgatctt cagtttaatc ctaaggacat tcctaaactg gttaagccca
     3181 ttatagaaga acattttaat tttgttactg gtactcgcta taaggatcat cgtgagtaca
     3241 atatgaaaaa ccgtggtttg aaaaattggg gtaacaaggt ttttgttcga attattaact
     3301 ggattaccgg ccataattat actgatgttt cctgtggttt tcgggcctat tcccggaaag
     3361 ccgccttgaa gcttcagcta ttcggccatt ttacctatac tcatgaaact tttctcgatt
     3421 tagttcacaa aggttttatt gcttgtgaag tgccggttaa agttcgtcca ttgcgtcaga
     3481 atggtcaatc ccatgtttcc ggcaatttat tcaaatatgc ttatagtgct ctgaaaatta
     3541 tcggtcgcag ttttcgtgac tatcacccat ttaagttttt tggctcgata tctttaattg
     3601 tctttttggt tggtttgatt cccattgtta tttttttcgt ccattatttt actaccggca
     3661 ggatgtcgcc gtttaaatct tatgctatcg gcggtgcttt tcttttggta atgagttttt
     3721 tgatttttat gctggccctt ctggctgata tgatgggacg aattaaagat cttgaagaag
     3781 aaattatcta ccaaatcaaa cgaaaaaaca aataaaaatc taacagttct cccttatcaa
     3841 aaggggaggg cccaaagggc gggggatttg ttatactagc aaatatgaat gttggaatta
     3901 ttggttttgg ccaaattggc cgagctatta aacaagttgt tgagaaaaaa catcgggttt
     3961 ttgcccgtga tttagatttt gatcaaatta agaaaaatag aatagacatt cttcatttgt
     4021 gctatccttt cgataaaaat tttactatta acgccgttag atatattaaa gaaattaaac
     4081 cgaatttggt gattatcgaa tcgacagtta aagttgggac caccgaaaaa atttaccgaa
     4141 aaactggcgt gaatttagtt catagtccgg ttaggggggt tcatcctcac ctttatgagg
     4201 gagtaatgac ttttcccaag tatatcggct cgacgagtaa aaaagctggt attatagcta
     4261 aaaaatatta tcaggatttg ggaattaaag ttgaattatt tgatgattct cggacgaccg
     4321 aattggctaa actaatgtgc actacctatt atggttggaa tattattttt gaaaaagaaa
     4381 tgtataaatt atgccataaa tatggtgtta attttgatca agcttacagc cgttggaatc
     4441 aatcttacaa tcggggttat cggcaattgg gcatgggtta tgttgttcgg cctgttctaa
     4501 aacatatacc cggtccgatt ggcggccatt gtgttatttc taattgcggc ttaataacca
     4561 gttctattac gcgaattatc tccaaattca ataaaaacta ctagactgct atacttgttt
     4621 catgccatgg cgggctttct ttttccgtaa cttgactact aaacagacgc tgattaaaaa
     4681 tactttttgg ttgggattgg ccgaaatagt ggcgaaatta gtcatgtttg tggttactat
     4741 tttggttgtt cgctcttttg gcccgactaa tttcggccgg ttgaatttgg ctaccgctta
     4801 cgccgccatt attgtggttt ttgccgattt tggccttaat attattacta cccgggaaat
     4861 tgctaaaaat aaaagtgaaa aagataaata tctttccaat atttttacta ttaagttaat
     4921 aatttccctg attatctggc tgataactgt tttttgggtc cgcgatcgat taattctttt
     4981 agttttaacc tttagtctgc tccagaattt ttgcctttta cttggcgctg ttttttccgg
     5041 tttggaagcg atgcaataca attttatttc ccgatcaatc tattaccttg gcctcctagc
     5101 cgccgctgtt tgggtaaccc gaactaatcc taatccggaa agattgatga agagctatat
     5161 tttaaccact agtttagcct ttattgttac tttttttatg ctcaaaatca aaaaaattac
     5221 cttaaggccg gcttttgatt tttctttttg gcggaagatt tttaaggaaa ccctgccttt
     5281 tgtcggcagt agtattattg gcgtaattta tcttaattta gattcaattt taatcggccg
     5341 ttatttcggc cctgaacagg ttggttttta ccaaagcgcc tacaagatcc tttttgcctt
     5401 tcaaagcatc aatatagtga ataattctct ttttcccaga atttcggcct taatggcaga
     5461 ggaaaaatac actaccttga aaaaattaaa tgtggtggtt atttctcttt cccttttggg
     5521 tcttattccc ttaggtgttt taatgactat tctggctaaa ccaattatga gaattattta
     5581 tggttccatt tatgtttcgg ccgctccggc catggtttgc cttatttgga gcggcattat
     5641 tttctatttc cgcaattatg tcaataatct actggtggct gctaatcagc aatatcgggt
     5701 tttttattct ctcctggctg gcactaccgt caatgctttg tttaatttgt tgattactcc
     5761 aaagtttaat ttcatttatg cttcgattgc catggttttg tcggaattaa caatccttat
     5821 ttattcctct attcaatatg ctagaaaaga aaaataacac ctatgtgatt attggaacca
     5881 aagctcagct ggtaaaaatg gccccagtaa tgctcgaact agaaaaaaga aaaatcgatt
     5941 acacttttat ctctactggt caacatcagg aaacaataaa agatttaatc tccactttcc
     6001 aaataaaaaa accagatttt tatttatata acggcaaaga tattacaaaa atatggcaaa
     6061 tgttgatt
//
LOCUS       DAOOIE010000015         5985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_10791_length_5985_cov_2.895110, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000015 DAOOIE010000000
VERSION     DAOOIE010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5985
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10791_length_5985_cov_2.895110"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /gene="manB"
                     /locus_tag="PK131_00870"
     CDS             <1..344
                     /gene="manB"
                     /locus_tag="PK131_00870"
                     /inference="COORDINATES: protein motif:HMM:NF012626.2"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HPD44717.1"
                     /translation="TFESPAVLIWKFLEFISLQNKPVSQIIAPYRIYFNSGEINSEVK
                     SIPDTLAKIKEKYNDGELNEIDGITLTYPDFWFNVRGSNTEPLIRLTLEAKTKDLMEK
                     KRDEVLALIRE"
     gene            complement(331..1038)
                     /gene="pyrH"
                     /locus_tag="PK131_00875"
     CDS             complement(331..1038)
                     /gene="pyrH"
                     /locus_tag="PK131_00875"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013457721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPD44718.1"
                     /translation="MKYKRVLLKLTGELFGQDEGKTLNFDKIGEIAKCLAKIKNKYQT
                     EIAVVVGAGNIFRGRNTGDGADRVTGDYIGMMATVVNSLALLAELERNKMEARLMTLL
                     TIQSVTEPFIRRKALSYLKEGKIVILGGGTGNPYFTTDSAAALKALELNCEVILKGSN
                     VDGVYDQDPKINKKAKKFETITFKEVIQRNLGIMDQTALALCRAEKMPIIVFNINDLN
                     NIEKILNGERIGTLIHE"
     gene            1106..1939
                     /locus_tag="PK131_00880"
     CDS             1106..1939
                     /locus_tag="PK131_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44719.1"
                     /translation="MEQDRSLITLENYIDESLKTFKNSDLLRTFSVKIKDDKGRLVDC
                     FLEKGNKEIFPYQSCLKLHRGEEIYELIADEIEKGRRLIKDQQEKEEVIREVNFVMME
                     NHEALQSKILRNFLPAKTNIYCPNQDEPFRFKQEKLALFKAIYHKIRRLPLNLKIDLI
                     YRNIPLTFYIEHPYKLDESDLDFLDDKKESKNFFYRAVIQLKERPNETDKVSYPISPT
                     MVFHKVKIAGTYHFRDWNGSRTEYQELIKFLDEAALSYNQLNENFGYQPRLNHHQNCS
                     F"
     gene            1884..2927
                     /gene="buk"
                     /locus_tag="PK131_00885"
     CDS             1884..2927
                     /gene="buk"
                     /locus_tag="PK131_00885"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002834.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HPD44720.1"
                     /translation="MKILVINPGSTTTKIALFNDQEKIWSYQFDHNLDHQTGLEQRLV
                     LIQEYLQKNKIDQLEAVIGRGGMLPPLISGTYEVNLKMLDDLANRPLAVHASNLGAPL
                     AKAIADRFKAKAYIADPVTTDEIEPKHKITGFKEIKRYAAWHCLNHKAVARQYAADIG
                     RQYEDLNLIIGHLGGGFSFAAHKKGRAVNVINALAGEGVFTPERSGALPAADLIKLCF
                     SGKFKQEDLQKRVAGKGGLADLLGTKDVAYLRQNYDRLDQFTKDVLEEMIFGIARCLA
                     SLVPDFEGEKIDQILITGGLAQWPLITDKIKFLLNQVPIGISVYPGEKEMEALAAAVG
                     RVLKGTEKLQKYT"
     gene            2995..3555
                     /locus_tag="PK131_00890"
     CDS             2995..3555
                     /locus_tag="PK131_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44721.1"
                     /translation="MTLEALVGLSQKERSIQITRDLFNRVISYPGYPAYRDEIVAVFD
                     SCRDDKNALVIMMVDRGRQFYLKCFVDAVIGVVSYQLKTKIIRYDRISRSPDFNAFEF
                     KQLTLNYFNNLFSVKEIDTKWVRGSDTYRQFMSQYNKYRNNLIEAANTTWSAKTNAKL
                     GYFIVSPNDIAIDQVENSIRIQFRKN"
     gene            3673..4041
                     /locus_tag="PK131_00895"
     CDS             3673..4041
                     /locus_tag="PK131_00895"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPD44722.1"
                     /translation="MKTQNFNKGRLGEEIARNYLERKGYEIIEQNYRVDQGEIDLIAI
                     NNDWLVFVEVKMKVGDLYGTPEEMISKGKLWQIRRVAEIWLLNHEKMRQKYPQQRIDA
                     VCLVVDKDYQLVSLKHYDNI"
     gene            4050..4853
                     /gene="dprA"
                     /locus_tag="PK131_00900"
     CDS             4050..4853
                     /gene="dprA"
                     /locus_tag="PK131_00900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPD44723.1"
                     /translation="MASIFDKLHYRGNWNENLFKKTLAVVGTRQMTHYGERVIDKLMP
                     DLVANEVTIISGFMYGIDSAAHKKCLELGGKTIAVLGSGLNHLYPSSNQKLYQAIIDK
                     GGLVISEFDDDFKPTLWSFPQRNRTVARLSTIGILIIEAGVKSGSLITAKLGRQMGKK
                     VFAVPGPITSLVSAGTNFLIKNNLAKMVTDISDILETKSEFTQEKFLRDLDRTEEKIM
                     KLLANEELTIDEIAHKTGISIADLSVKLSMMAMKDYIEESNGKYLVIND"
     gene            4920..>5985
                     /locus_tag="PK131_00905"
     CDS             4920..>5985
                     /locus_tag="PK131_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPD44724.1"
                     /translation="MLTKVLSVANYGLKAVNVDVEVDIARKGFPGIGIVGLASRTGEE
                     TKERVKTAIINSDFDFPNAKITINLAPADLPKNGPGYDLPIAVGLLASMAEITLPEEK
                     SYFYGELSLDGSLRHTKGVFLLAIQAKEEGVKNIFVPRLCANEAAVINDLNVYPVDDL
                     RSLIRHLRGERLIEPLKIINPEDLLTDIHPEFDFTEVLGQEQAKRALEIAAAGGHNLF
                     MMGTPGAGKTMLSRAMPGILPDLTEKESLEVTKIYSVTGNIPPGASLVRHRPFRSPHH
                     TTSTVALIGGGTNPHPGEVSLAHRGVLFLDEFPEFNRDCLEALRQPLEDGHISIARAA
                     GSVDFPAQFILVAAANPCPCG"
BASE COUNT         1808 a         1079 c         1231 g         1867 t
ORIGIN      
        1 gtacttttga atcaccggca gttttgattt ggaaattttt agaatttatt tctcttcaaa
       61 ataagccggt ttctcaaatc attgctcctt atcggattta ttttaattcc ggcgagatta
      121 actccgaagt taaaagtatt cctgatactt tggctaaaat caaagaaaaa tacaatgacg
      181 gtgaactaaa tgaaattgac ggtatcactc ttacttaccc cgatttctgg tttaatgtcc
      241 ggggaagcaa taccgagcca ttaattcgct tgaccctgga agccaaaacg aaggatttga
      301 tggagaaaaa aagggacgaa gttttagcac ttattcgtga ataagcgttc cgattctttc
      361 gccgtttagg atcttttcga tattatttag atcgttgata ttaaagacaa ttatcggcat
      421 tttttctgct cggcataaag ctagagctgt ttggtccata atgcctaaat ttctttggat
      481 tacttcttta aaagtaattg tctcaaattt ttttgccttt ttattaattt taggatcttg
      541 atcataaact ccatcaacat tacttccttt taaaataact tcacagttta actccaaagc
      601 tttgagggcg gcggcactat cagtagtgaa gtaaggattg cctgtccctc cacctaaaat
      661 aacaattttg ccttccttta gataacttaa agccttcctt ctaataaagg gttcagtcac
      721 actttgaatg gttagtaaag tcataagtcg ggcctccatc ttatttcttt ccaattccgc
      781 caaaagggcc aaggagttaa ctacagtcgc catcatcccg atatagtcgc cggtaacccg
      841 gtcagcacca tcaccggtat ttctacctcg aaagatattg ccagcaccaa caaccaccgc
      901 aatttctgtc tggtatttat ttttaatttt agccagacat ttggcaattt caccaatttt
      961 atcgaaatta agcgttttac cttcgtcttg accaaacaac tcgccagtta atttgagcag
     1021 gactcttttg tatttcataa attaagtata gcaaactaga caatggggat tgttttcagg
     1081 aattatgtgc tataatcccg ctaaagtgga acaagacaga agtttgatta ccttagagaa
     1141 ttatattgat gagagcttaa aaactttcaa gaacagcgat ttattaagaa ccttttccgt
     1201 caaaattaag gatgacaagg gtaggttagt cgattgtttt ttggaaaagg gtaacaagga
     1261 aattttccct taccaatcat gtttaaaatt acatcgaggt gaggaaatat atgagctgat
     1321 cgcggatgaa attgaaaaag gaagaagact gattaaagac cagcaagaga aagaagaagt
     1381 gatcagagaa gttaattttg ttatgatgga gaaccatgag gccttacaat caaaaatttt
     1441 acggaatttt ctccctgcta aaaccaatat ttactgtccc aatcaagacg aaccatttcg
     1501 atttaaacaa gaaaaattag ccttatttaa ggctatttat cataaaataa gacgattgcc
     1561 gctaaattta aaaatagatt taatctaccg caacattcct ttgacttttt atattgagca
     1621 tccttataag ttagatgaaa gtgatttgga ttttttggac gataaaaaag aatctaaaaa
     1681 ctttttctat cgggccgtaa ttcaattaaa agaaagaccc aacgaaactg ataaagttag
     1741 ttatccgatt tccccaacaa tggtttttca taaagttaaa attgctggta cttatcattt
     1801 tcgtgattgg aatggttcca gaactgaata tcaggaatta attaaatttc tcgatgaggc
     1861 tgccttaagc tataatcaac ttaatgaaaa ttttggttat caaccccggc tcaaccacca
     1921 ccaaaattgc tctttttaac gaccaggaaa aaatttggag ttatcaattt gatcataatc
     1981 ttgaccatca gacggggttg gaacagcgtt tagttttaat tcaggaatat cttcagaaaa
     2041 ataaaattga ccaacttgag gcagttattg gccgtggggg aatgttgccg ccgttaattt
     2101 cgggaactta tgaagtcaat cttaagatgc ttgacgattt ggcgaatcgc cccctggcgg
     2161 ttcacgcttc taatttggga gcacctttag ctaaagccat tgctgatcga tttaaagcca
     2221 aggcttatat tgctgatccg gttactacag atgaaattga acctaaacat aaaattaccg
     2281 gttttaagga aataaaacgt tatgccgctt ggcattgtct taaccataaa gcggtggccc
     2341 ggcaatatgc tgccgacatt ggccggcagt atgaagattt aaacttaatt atcggccatt
     2401 taggcggcgg tttttccttc gccgcccaca aaaaaggccg ggcggtcaac gttattaatg
     2461 ctctggccgg cgagggagtt tttactcctg aacgctctgg ggctttaccg gcggccgatc
     2521 taattaaact ttgtttttcc ggcaaattta aacaagagga tttgcagaaa agagtagctg
     2581 gcaaaggcgg tttagccgat cttttgggga caaaagatgt cgcttatctc cgtcaaaatt
     2641 atgaccgtct cgatcaattc accaaagatg ttttagaaga aatgattttc ggcattgccc
     2701 gctgtctggc ttctctagta cccgattttg aaggtgagaa aattgatcag attttaatta
     2761 ccggcggttt agcccaatgg ccgctgatta ccgataaaat taaatttctt ttaaatcagg
     2821 ttcctatcgg tatttctgtt tatcctggtg agaaagaaat ggaggctctg gccgccgcgg
     2881 ttgggcgggt gttgaaaggt acggaaaaac tccaaaaata tacatagtcg tggtgaagtt
     2941 ttctcgcctt ttctatctct tgacaaatag cctataatat gatataatta acttatgacc
     3001 cttgaagcgt tagtcggttt aagtcagaaa gagcgatcaa tccaaattac ccgtgattta
     3061 tttaatcggg ttatcagtta tcctggttat cctgcttata gggatgaaat tgtagccgtg
     3121 tttgattctt gccgggatga taaaaacgcc ttggtaataa tgatggttga tcgcggccgg
     3181 caattctatc ttaaatgttt tgttgatgct gttattggcg ttgtgagtta tcaactaaaa
     3241 acaaaaataa ttcggtatga ccggattagt cgttcgcccg attttaatgc cttcgaattt
     3301 aaacaactga cccttaatta ttttaataat ttattttctg tcaaagaaat tgatactaaa
     3361 tgggtcaggg gcagtgacac ttacagacaa tttatgagtc aatataataa atatcgaaat
     3421 aatcttatag aagcggccaa taccacttgg tcagccaaaa ctaacgctaa attaggttat
     3481 tttattgtta gtcctaatga tattgcaatt gatcaagttg aaaatagtat cagaattcaa
     3541 tttcgtaaaa attaacaaaa cagtataata aaagggtgaa gtcaacttat tggctgattt
     3601 tttttctggc cgctttagtt acttttttaa ctttagtgag atatttttat ttggccggct
     3661 ggttttaagg taatgaaaac acagaatttt aataaaggcc ggctgggaga agaaatagcc
     3721 cgtaattatt tagagcgaaa aggatatgaa attattgaac aaaattaccg ggttgatcag
     3781 ggagaaattg atctgattgc cattaataac gactggttgg tttttgttga agttaagatg
     3841 aaagttggtg atttatatgg caccccggag gagatgattt ccaagggtaa gctttggcaa
     3901 atccgacggg tagcggaaat ttggctgtta aaccatgaaa aaatgagaca aaaataccct
     3961 cagcagagaa tcgatgctgt ttgtttggta gttgataaag attatcaact ggtttcctta
     4021 aaacactatg acaatatcta attagaatca tggcttctat ttttgataaa cttcactacc
     4081 ggggtaattg gaatgaaaat ctttttaaaa agacactagc agtagtgggt accaggcaaa
     4141 tgacccacta tggggagagg gttatcgaca aattaatgcc cgatttggtc gctaatgagg
     4201 taactattat ttccggtttt atgtatggaa ttgacagcgc cgcccataaa aaatgcttgg
     4261 aattaggagg caaaactatt gccgttctcg gcagtggtct taatcacctt tatccgtcga
     4321 gcaatcagaa gctttatcag gcaattattg ataagggcgg tttggttatt tcggaatttg
     4381 acgatgattt taaaccaacc ttatggagtt ttcctcaaag aaatagaacc gtcgcccgat
     4441 tgtcaactat tggaattttg attattgagg ccggagttaa atcgggcagt ttaattacgg
     4501 ccaaactggg gcggcaaatg ggtaaaaaag tttttgctgt tcctgggcca atcacttctt
     4561 tggttagtgc cggcactaat tttttgatta aaaataattt agctaaaatg gtcactgaca
     4621 tcagtgacat tttggagact aaaagcgaat ttactcagga aaaattttta agggatttag
     4681 atcgaaccga agaaaaaatt atgaaattac tggccaatga agaattgact attgatgaaa
     4741 ttgcccacaa aaccggtatt agtatcgccg atttgtcggt aaaactatca atgatggcga
     4801 tgaaggatta tatagaggaa agtaatggca aatatctagt aattaatgat tagcaactcg
     4861 ctattgggca ggcaggattt ttaattcgta attcgtaatt tttaattata aaatagattg
     4921 tgttaaccaa agttctgtcg gtggctaact acggcctaaa agccgttaat gttgatgttg
     4981 aagttgatat tgcccgaaaa ggctttccag gaatcggtat tgttggttta gcttccagaa
     5041 ccggtgaaga gactaaagaa cgggttaaaa cagccattat taattctgat tttgatttcc
     5101 ctaacgctaa aatcactatt aatttagcgc cggccgattt acccaaaaac ggccctggtt
     5161 atgatttacc cattgctgtc ggtttactgg ctagtatggc tgaaattact ttgccggagg
     5221 aaaaaagtta tttctacggc gaactgtcac ttgatggaag tttgcgccac accaaaggtg
     5281 ttttcttatt agccattcag gccaaggaag aaggggttaa gaatattttt gttccccggc
     5341 tttgtgctaa tgaggcagcg gtgattaatg atttaaatgt ttatccggtg gacgatttaa
     5401 gatcacttat tcgtcatttg cggggagaga ggctgattga accgctgaaa attattaatc
     5461 ctgaagatct tttgactgat attcaccccg aatttgattt tactgaagtt ttaggtcagg
     5521 aacaagccaa acgagctttg gaaatcgccg ctgccggcgg ccataattta tttatgatgg
     5581 gaactcccgg tgccggtaaa accatgctat cccgggctat gccgggaatt ttacccgatt
     5641 taacggaaaa agaaagttta gaagttacta aaatttattc ggttactggt aatattccgc
     5701 cgggggcttc attagttcgt caccggccat ttcgttcgcc tcaccatacc acttcaacag
     5761 tagctctcat tggcggcggt actaatcccc atcccggtga agtttcctta gcccatcgcg
     5821 gcgttttatt tctggatgaa ttccccgaat tcaaccggga ttgccttgag gctcttcgac
     5881 agccattgga agatggtcat atctctattg cccgggcggc cggttcagtc gattttccag
     5941 cccaatttat tttggtagcc gccgctaatc cttgtccctg cggct
//
LOCUS       DAOOIE010000016         5965 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_10838_length_5965_cov_3.697462, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000016 DAOOIE010000000
VERSION     DAOOIE010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10838_length_5965_cov_3.697462"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="PK131_00910"
     CDS             complement(<1..1371)
                     /locus_tag="PK131_00910"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPD44725.1"
                     /translation="MKKYDFKLIEKKWQDYWRDNNIFKFDPQSPKPIFSIDTPPPTVS
                     GRMHLGHVFSYTQTEIIARFFRMAGYNVFYPIGMDDNGLPTDKLAEKELGIKSASLPR
                     DEYRQQVQTLVSRYHQDYMNLFNSLGFSFDWHLLYSTISSEVQELTLKNFEDFKKRNI
                     IYKKKAPCLWCPACGTGVAQAEVEDRELETVFYDLQFGDLIISTTRPELLPACVAVFV
                     HPDDVRYQRLIGTETTTSLGDKVKILADGKVKPDKGTGAVMICTYGDETDWYLKNKYQ
                     LDEKIIIDEQGRIDGLTIPAAREKIIKELKSQNLVVKETPLTHDVGVHERCGTVIEII
                     LREQYFLKVLEIKEEILAMAETINWYPAFMKERLIDWVKNLKWDWCLSRERPYGIPIP
                     GEDNLVFDTWFTSANTPEINTLKGGRIPYSLRPQAYEIIRTWAFYTLVLSYYKKQGIP
                     WQNVAVS"
     gene            complement(1380..1877)
                     /locus_tag="PK131_00915"
     CDS             complement(1380..1877)
                     /locus_tag="PK131_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44726.1"
                     /translation="MSKTLEHYHNLVEQDRSQGRLKIIFNGLKNGLDNAVIDRQLAAY
                     DYRLETGQKRGQKSVNRVIKAIKEEKIIGEIRQIKDFSGEDIYQKRDLAVVINNYHGF
                     HDQIWIQVKSSCREVENFYTLIDKNGHQAQQILTERKLIVLNGNISPSRIRSEFYRQL
                     DLLIK"
     gene            1927..2928
                     /locus_tag="PK131_00920"
     CDS             1927..2928
                     /locus_tag="PK131_00920"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPD44727.1"
                     /translation="MPATKKQIIQILKMGGLVVIPSDTVYGLAVDATQSKAVEKLLKF
                     KERWPGKAIAVFCDSFKMASDYAIINNNVQTIYTNWLPGPFTLIVEGRHRLAKGIEAE
                     DGSLGIRIPNYPLIIEVVKEFGRPITATSANLSGHRPHYSTTSFLKTLSEKKRRMIDL
                     IVDVGKLPRNKPSTVVDIREPKIKILRRGDFITPNRQTFISHSAQETQKIAGTLLSKI
                     PQPIILGLSGDLGTGKTVFSQGIGRAMGIKEKITSPTFTIYNDYGKFLHIDLYRINNQ
                     EELMELNIFNAIKNKKVVCIEWIENLGKTNLVKLTQIAPLKFLTFKYIDEHTREIKL"
     gene            2925..3956
                     /gene="tsaD"
                     /locus_tag="PK131_00925"
     CDS             2925..3956
                     /gene="tsaD"
                     /locus_tag="PK131_00925"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPD44728.1"
                     /translation="MKILAIDTSCDDTCAAVVENRKIITNIVSSQINLYKDWGGVVPN
                     IAKWAHKKRIDLVINKALNLHLTPSSKKRESINSIDVIAVTQGPGLAVALEVGIDKAK
                     ALAKQYKKKLIAVNHIEGHLLSTLFNHQIAFPALGLTVSGGHSQIILIHSIGDYRIIG
                     ETLDDAAGEALDKAARILGLGYPGGPIIERLAQKGNKNFLKLPKVLPDNKILNFSFSG
                     LKTSFYYQIKNLSPQQISSHLSDYAASYQEAVFEQLIRKFRLAVEKYQPKVLLAAGGV
                     MSNLELRNKLRQLAKTLNLPINMPYNQKLNTDNAAMIGLTAYYKALKGEFVEDIDKLD
                     RKPRLKLDY"
     gene            complement(3969..5684)
                     /locus_tag="PK131_00930"
     CDS             complement(3969..5684)
                     /locus_tag="PK131_00930"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HPD44729.1"
                     /translation="MNRKLLNWLLLIFLVFLLISSPLVINFGKTGRRISLLGKDYSLL
                     SKREIVARLETDFSLPRDLKLIDGNRILIINTADISAVIDKNKTASSLLFRRLKQGPW
                     RYLTAFFQPKDFKLIFQINQDQLHQRLDWVAREIDRPFIPAEFYINENHQVKVNNGEI
                     GRRVDQASLKTIILEALANYQFNQPIIIPVTNVGSLPDQNQLASSLKEAQKIIGRSVV
                     LIGENQEIILSDDLLISWYDFNNTYKTDKISEYVDNLASSLKKEPVDAVFQFENGHVL
                     AFRPAQKGYTLNVSQLVSLLNRRLPGLTSQTETQLRLDLPFIYSDPKVNNADVNNLGI
                     KELIGRGSSSFKTSASYRNVNIAQGAAIVNRILVAPGETFSFNKALGKVDAESGFGKA
                     YIIKQGRTVLDIGGGICQVSTTLFRALLNAGLNITERQAHAYRVGYYEEDMPPGYDAT
                     VFSPKPDLQFINDTDHYLLIQSLYDGVEKKLTYEIYGTSDNRKVEITNYRQWGKVPPP
                     PDIYLDDPSLPPGKIVQDEHKVSGLKVAFDWRVIKSGEVLYQRTFTSSYVPWAAIFPR
                     GPISQ"
     gene            complement(5665..>5965)
                     /locus_tag="PK131_00935"
     CDS             complement(5665..>5965)
                     /locus_tag="PK131_00935"
                     /inference="COORDINATES: protein motif:HMM:NF024848.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="reverse transcriptase-like protein"
                     /protein_id="HPD44730.1"
                     /translation="NEAEYLAMKMALEWVKSNTNRYQITQMDFYADSQLLIYQLQGLY
                     KVKASNLKSLWADCRQLLQDLNLPYSFNLIRREENSLADKLANQSMDSAYESKTT"
BASE COUNT         1977 a         1142 c         1046 g         1800 t
ORIGIN      
        1 agaaacggcc acattttgcc aaggaatacc ctgtttctta taataagata ggactaaagt
       61 ataaaaagcc caggtacgaa taatttcata ggcctgaggg cgaagagaat aagggatccg
      121 gccgccttta agagtattta tctctggagt attggccgaa gtaaaccaag tatcaaaaac
      181 caaattatcc tcaccgggaa tgggaatacc gtaaggccgt tctcgcgaca aacaccaatc
      241 ccattttaga ttttttaccc aatcaatcaa acgttctttc ataaaggctg gataccaatt
      301 aattgtttcc gccatcgcta aaatttcctc tttaatttcc aaaactttaa gaaagtattg
      361 ctctcgcaaa ataatttcaa tgactgttcc acaacgttca tgaaccccca catcatgggt
      421 taatggtgtt tctttcacta ctaaattctg cgattttaat tcctttatta tcttttctct
      481 ggcggctgga atagttaacc cgtcgatccg accctgctcg tcaataatta tcttttcatc
      541 caattgatat ttatttttaa gataccaatc ggtctcatca ccataagtgc aaatcataac
      601 cgccccggtg cctttatcag gtttgacctt accatcagcc aatatcttaa ccttatcccc
      661 cagggaagtg gtggtctctg ttccaattaa acgttgatat ctcacgtcat cgggatgaac
      721 aaaaacagcc acacaagctg gtaataattc cggccgggta gtagaaatga tcagatcgcc
      781 aaattgaaga tcataaaaaa ctgtttctaa ttccctatcc tcaacttcag cttgagccac
      841 accagtgcca caagcaggac accataaaca tggggctttc tttttataaa taatgttcct
      901 ctttttaaaa tcctcgaaat tcttcagggt taattcctga acttcagaag aaatagttga
      961 atataagaga tgccagtcaa aagaaaaacc aagagaatta aacaaattca tgtaatcctg
     1021 atgatagcgg ctaactaaag tttgaacctg ctggcggtat tcgtcacgag gtaaactggc
     1081 agatttaatc cccaattctt tttcggccag tttatcggtt ggcaaaccat tatcatccat
     1141 tccgattgga taaaaaacat tatagccggc catgcgaaaa aaacgcgcta taatctcggt
     1201 ctgggtataa gaaaaaacat ggccgaggtg catcctgccg gaaacagttg gcggaggggt
     1261 atcaatagaa aaaatcggtt ttggtgattg gggatcaaat ttaaaaatat tattgtcgcg
     1321 ccaatagtcc tgccattttt tctcaattaa tttaaaatca tattttttca tcaacaaatt
     1381 tattttatca gaagatctaa ttggcgatag aactcactcc taattctcga aggggaaata
     1441 ttaccattta aaacaattaa ttttctctca gtaagaattt gttgggcttg atgaccattc
     1501 ttatcaatta aggtgtaaaa gttttccact tcccgacagc tggatttaac ctgaatccaa
     1561 atttgatcat gaaaaccatg ataattattg ataacaacgg ctaaatcgcg cttttgataa
     1621 atatcttcac cagaaaaatc ctttatctgc cgaatttcac caattatctt ttcctcttta
     1681 atggctttaa taactcgatt aactgacttc tgaccacgtt tttgccctgt ctcaaggcga
     1741 taatcgtaag cagctaattg acggtcgata accgcattat ctagaccatt tttcaaacca
     1801 ttaaaaataa tttttagacg tccttggcta cgatcctgtt ctactaaatt atgataatgt
     1861 tctaaggttt ttgacataga ttaaaagttg catcgattat agcataatga tttgataaaa
     1921 ttggctatgc cagccactaa aaaacaaatc atccagattt taaaaatggg tggtttagtc
     1981 gtcattccct cagatacggt ttatggatta gcggttgatg cgactcaatc aaaagccgtt
     2041 gaaaagttat taaaatttaa agagagatgg cccggtaagg ctattgccgt gttttgcgat
     2101 agctttaaaa tggctagtga ttacgcaata attaacaaca acgtacaaac aatttataca
     2161 aactggttac ccggtccttt tactttaatt gtcgagggcc gacatcgatt agctaagggt
     2221 atcgaagcag aagatggttc tttaggtatt agaattccca attatccttt aatcattgaa
     2281 gtggttaaag aatttggccg gccgattacc gctacttcag ctaacttgtc aggccatcgg
     2341 ccgcactact caactaccag ctttcttaaa actttatccg aaaaaaaaag acggatgatt
     2401 gatttaattg ttgatgttgg taaactacca cgaaataaac catcaacagt agtcgatata
     2461 agagaaccga aaataaaaat tctccgtcga ggggatttca taactcctaa taggcaaaca
     2521 ttcatttccc attcagctca agaaacccaa aaaattgccg gaactcttct ttctaaaata
     2581 ccccaaccaa ttattttagg tttaagcggt gatttgggta caggtaaaac tgttttttct
     2641 caaggtattg gccgagctat gggaattaag gaaaaaatca cctcccccac ttttactatt
     2701 tataatgact acggcaaatt cctacatatt gatctttatc gaattaataa tcaggaagaa
     2761 ttgatggaac tcaatatatt taatgccatt aaaaataaaa aggtggtctg tatcgaatgg
     2821 atagaaaatt taggtaaaac caatttggtt aaattaactc aaatagctcc tttaaaattc
     2881 ttaactttta aatatattga tgaacatacc cgagaaataa aattatgaaa attttagcta
     2941 ttgataccag ttgtgatgat acttgtgccg ccgtagttga aaatcgaaaa ataatcacca
     3001 atattgtttc atctcaaatt aatttatata aggattgggg tggagttgtt cctaacatcg
     3061 ctaaatgggc tcacaagaaa aggattgatt tagtaataaa taaggcatta aatctccatc
     3121 taaccccctc ttcaaaaaag agagagagta tcaatagcat tgatgtgatt gccgttactc
     3181 agggaccggg gctagcggta gctttggaag ttgggattga taaggccaaa gcattggcta
     3241 aacaatataa aaaaaaacta atagccgtca atcatattga gggtcattta ctatctactc
     3301 tttttaatca tcaaattgct ttcccagctc ttggtttaac tgtttccggt ggtcatagtc
     3361 aaattatcct tattcatagc attggtgatt acagaataat tggagaaact ttagatgatg
     3421 ccgccggcga agctttagat aaagccgcta gaatattggg gttaggttat cccggcggac
     3481 caataatcga acgattagct caaaaaggaa ataaaaactt tctcaaacta cccaaagtcc
     3541 ttcctgataa taaaattcta aattttagtt tttctggatt aaaaaccagt ttttattatc
     3601 aaattaaaaa tttatcaccc cagcaaatta gtagccattt atcagattac gccgccagtt
     3661 atcaggaagc tgtttttgaa caattgatcc gtaaatttag actagcggtt gaaaaatatc
     3721 aaccaaaagt attactagcc gctggcggag ttatgtctaa tttagaatta agaaataaat
     3781 taagacaatt agccaagact ctcaatctgc caataaatat gccctataat cagaaactga
     3841 atacggataa tgcggcaatg atcggtttaa ccgcctatta caaagctctg aaaggagaat
     3901 tcgttgaaga tattgataaa ctcgatcgta aaccccgcct taagttggat tactaaaaag
     3961 aagcggccct attgagatat tggtccccgg ggaaaaatgg ctgcccaagg aacataggac
     4021 gaagtaaatg tccgttggta aagcacttca ccacttttta taactctcca atcgaaagct
     4081 acttttagtc cgctgacctt atgctcatct tgaactattt tcccgggcgg caaactagga
     4141 tcgtcaagat aaatgtctgg tggtggtgga actttacccc attgacgata attagtaatt
     4201 tctaccttcc gattatcact agtgccataa atttcataag tcaatttttt ttccacccca
     4261 tcataaaggc tttgaataag cagataatga tcagtatcat taataaattg caaatcgggt
     4321 ttaggtgaaa agacagttgc atcataacct ggcggcatat cttcctcgta ataaccaacg
     4381 cgataggcat gagcctgacg ttcggtaata ttcaaacctg catttaatag ggctcgaaac
     4441 aaagttgttg atacttgaca gataccaccg ccaatatcta aaacagttcg cccttgttta
     4501 ataatataag ctttaccaaa tccactttca gcatctactt taccaagagc tttattaaaa
     4561 gaaaaagttt cgcctggagc aactaaaatt cgattgacaa tagctgctcc ttgggcaata
     4621 ttaacattgc ggtaactggc actggtttta aaggaagaac tacctcgacc aatcaattcc
     4681 ttgatgccta aattattaac gtcggcatta ttaactttag ggtcactata aataaagggt
     4741 aaatctaatc ttaattgagt ctcggtttga ctggttaaac ctggaagacg acgatttaac
     4801 aaagaaacta attgggaaac attaagagta tatcctttct gagccggacg aaaggctaaa
     4861 acgtgaccgt tttcgaattg aaaaacagca tccactggct cttttttcaa actggaagct
     4921 aaattatcaa catactcgct aattttatcc gttttatagg tattattaaa atcataccaa
     4981 gaaataagca aatcgtcgct cagaataatt tcctgattct caccaattaa aactactgac
     5041 cggccaataa ttttttgagc ctccttgaga ctagaagcta attgattctg atctggcaaa
     5101 gaaccaacgt tagtaactgg aataataatt ggctgattaa actgatagtt ggctaaagcc
     5161 tctaaaataa ttgtttttaa agaagcttga tcaacccgtc ggccaatttc accattgttt
     5221 acttttactt ggtggttttc attaatatag aattcagctg gaataaatgg tcggtcaatt
     5281 tctctagcta cccaatcaag ccgctgatgg agttgatctt gattaatttg aaaaatgagt
     5341 ttaaagtctt taggttggaa aaaggcagtt aaatagcgcc atggaccttg ctttaatcgc
     5401 cggaacaaaa gactggaggc cgttttgttt ttatcaataa ctgcagatat atcggctgta
     5461 ttgatgatta aaatccgatt gccatcaatt aatttcagat ctctgggtaa agaaaaatca
     5521 gtttccagcc tggctacaat ctcccgtttg gataacagag agtaatcctt acccaaaagg
     5581 ctaattcggc ggccagtttt accgaaatta attactaaag gtgaactaat taaaagaaaa
     5641 actaaaaata tcaaaagaag ccaattaagt agttttcgat tcataggcag aatccattga
     5701 ttgattagcc agtttatcgg ccaaagaatt ctcctcccgg cgaattaaat taaaagaata
     5761 aggtaaattc aaatcttgga gtaactggcg gcaatcggcc caaagggatt tcaaattaga
     5821 agctttaact ttataaagac cttgtaattg ataaatcaaa agttgagaat cggcataaaa
     5881 atccatttgg gtaatttgat atctattggt attactttta acccattcta gggccatttt
     5941 catggcaaga tattcggctt cgtta
//
LOCUS       DAOOIE010000017         5636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_11564_length_5636_cov_2.865257, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000017 DAOOIE010000000
VERSION     DAOOIE010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11564_length_5636_cov_2.865257"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1159
                     /locus_tag="PK131_00940"
     CDS             <1..1159
                     /locus_tag="PK131_00940"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPD44731.1"
                     /translation="RPELARVIAEVDSLKQNLLENTRLKNTLVEQEQMLVEKNTVLAQ
                     RIGLFETDAESYEKQLTLVQENIHSIRRAVEECAATLSNLRVTEAGLAHRLDELNREI
                     ERYQREITELDQDDEKRRQTLGNLDEQKRNLKTLLFYLLPPFILIILEPNLSTAVLIS
                     AICLVIYYLSGASFQSIVAFSCLSLVIFSLLTFLAPYRFARLKTFLKPEDNSSTATYH
                     RNQIIYALASGGIRGKGLANSDQKYKYLPKISTDSILAIIGEETGFIGLSLLFYIYLL
                     LIGYLFRLAGQITNPFSSLLIGGIGSWIAMQTLINAGAIANIIPLTGIPLPFISYGGS
                     SLVTLFFSLGLVHNIEKNDNNLIYSKHGPNAGHYRYSSYSRLRTHRTTSGR"
     gene            1083..2156
                     /locus_tag="PK131_00945"
     CDS             1083..2156
                     /locus_tag="PK131_00945"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD44732.1"
                     /translation="MDQTLVITGTHLTPACELIEQLRADKQFRWKIIYLGRRYNSLSP
                     VPSIESIVIPRKKIKFYGLDCGKFDRKSIKTTLKGLPLIFKGICQAFKILKEEKPNII
                     ISFGGYVSVPVIIAGWLLKIPSITHEQTPTLSLATRINSFFVNQIALSFPHPGLKKAV
                     VTGNLLRRELTNHHKKLFLTQNKTLIYVTAGNQGSHFLNNLIKITLPKLNDFYIIHQT
                     GNIDYHEYKKLERQYSNYRVYSYLSDSIVGSVFYQAEIIISRAGANTSQEIVALKKKS
                     ILIPLPFSQQNEQLLNAQFVRQQLPDRTIIIPQSEINPAILVKTIKSLLPIAPYKLKP
                     IKPNLKLLKLIQSLCKKRLKSLE"
     gene            2167..3417
                     /gene="ftsA"
                     /locus_tag="PK131_00950"
     CDS             2167..3417
                     /gene="ftsA"
                     /locus_tag="PK131_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPD44733.1"
                     /translation="MQHSKIISGIDIGSSKITTIIGQYFEQENRFNVIAVASSPAVGF
                     RKGQIINLEQAAVTLTQSVESAERMAGLQINNAYVASAAAHIESFNNKGVVAVNNQNG
                     EIAVEDIHRVIEATQTISLPTGKEILHIIPRDYTVDGQEGIIDPIGMTGSRLELEAHL
                     ILASTPAIKNIKKCLMDIGISINDLIYSGLAAAKSALTDTEKELGGALVDIGGSITTL
                     TLFYESSPALSAVIPIGANNITNDIAIGLRFSLENAEKIKVQLGKVMENKKFEDEIEL
                     AHFGVINESQKKLSLQTTINGIIKPRLEEIFSIILGKIQNSGYYNLIPAGIVLTGGGA
                     LTVNAKEICSKIIGLPVRLSQPPSIGGMVDDVLSPAYTSAVGLLFYFLDDKRKFNFPT
                     IGRSHHSFSNVFSKIKSLIKPLLP"
     gene            3423..4544
                     /gene="ftsZ"
                     /locus_tag="PK131_00955"
     CDS             3423..4544
                     /gene="ftsZ"
                     /locus_tag="PK131_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPD44734.1"
                     /translation="MPLIKTLAGQFAKIKVMGIGGAGNNAINSMIEEDIIHGVDFIAV
                     NTDSQALLNSKAMVKLQIGEKLTKGLGAGGNPKIGEDAAQESQERIKELLEGTNLLFI
                     TGGMGGGTCTGAAPVIAQIAKKELGILTIAVVTKPFLFEGARRLANAEEGISLLKDKV
                     DTLIVIPNQKVMEVIDNQATLLEAFKVADSILNKGTRAIADLITVPGLINLDFADIKS
                     IMSDAGSALMGVGEAEGENRVTAAVEAAINSPLIEVNIDGARGVLINITGGPDLTMTE
                     VETAAKAITARTSPDANIVFGYTIDSNLKGKMKITVIATGFDTNTQRIYQNSTPTTNQ
                     SSLEINQETIDKYLGNQEIPKGIDINDEFDIPAFLRKVK"
     gene            4589..>5636
                     /locus_tag="PK131_00960"
     CDS             4589..>5636
                     /locus_tag="PK131_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPD44735.1"
                     /translation="MSSFKSLPANLDFPALEEEMLQWWQKHDTVSQYLHRNDQSKKRF
                     SFIDGPITANGPMGVHHARGRTIKDVFQRYKNAQGFAQRFQNGFDCQGLWVEVEVEKE
                     NGFNCKKDIKEFGLAKFTQSCIDRVKNYAALQTKQSQRLGMFMDWDNSYYTFSETNNL
                     YIWYFLKKIKEKGWLYRKKAATTWCPRCETGLSQHEQADSYQTVKETSVYLKFKIKNK
                     TDEYILAWTTTPWTLPANVLLAINPKYDYVKAKYKGEKIYLAKKAADRLKLTDYEIIK
                     AHQLLGVEYESLYHIPAQEGIHHAVVQWDAVDPADGTGIVHIAPGCGQEDYELGLSLG
                     ADIISPIDETGHFIS"
BASE COUNT         1753 a         1148 c         1056 g         1679 t
ORIGIN      
        1 tcggcctgaa ctggccagag taatcgccga ggtggactcg ctcaagcaaa atcttctgga
       61 gaacacccgt ctgaagaata cgctcgttga gcaagaacag atgttggtgg aaaagaacac
      121 agtgttggca caacgcatag ggctgtttga aaccgatgcc gaaagctacg aaaagcaatt
      181 gacgctggta caggagaata tccacagtat ccgacgcgct gtagaagaat gtgcggcaac
      241 tctgtctaat ttacgagtta cggaagcagg tttggcacac cgtctagacg aactcaaccg
      301 cgaaatcgag cgctatcagc gtgaaataac ggagctggat caggacgacg aaaagagacg
      361 ccagaccctt ggaaacttgg acgaacagaa aagaaatctt aaaactcttc ttttttatct
      421 cttaccacca tttatcctta ttatccttga gccaaatctc agtacggccg ttttaatttc
      481 ggctatttgt ttggtcattt attatctctc cggcgcttct ttccaatcaa ttgttgcctt
      541 tagttgtctt tctctagtta ttttcagtct attaactttc ttggctcctt atcgctttgc
      601 ccgtttaaaa acctttctta aacccgagga taattccagc actgccactt atcaccgcaa
      661 ccaaattatt tatgctctgg cttcgggagg gattcgtggt aagggattag ctaactcgga
      721 ccaaaaatac aaatatctcc ctaaaatttc taccgattcg attctagcca ttattggcga
      781 ggaaactggt tttattggtc ttagtttact tttctatatc tatcttctcc tcattggcta
      841 cttattccgc ctcgccggcc aaataaccaa tcctttttct tcccttttaa tcggcggtat
      901 tggtagttgg attgccatgc aaaccctaat taatgccggg gctatcgcaa atattattcc
      961 tttaaccggc ataccccttc cttttatttc ctacggcgga tcatcattag tcactctatt
     1021 tttcagctta ggactggtgc ataatatcga aaagaatgat aataacctga tatactcaaa
     1081 acatggacca aacgctggtc attaccggta ctcatcttac tcccgcctgc gaactcatcg
     1141 aacaacttcg ggcagataaa caattccgat ggaaaattat ttatcttggc cgacgataca
     1201 attcattatc acctgttccc tcaattgaat cgatcgttat tcctcgaaaa aaaattaaat
     1261 tttacggcct tgattgtggt aaatttgacc gaaaatcaat caaaacaact ttgaaaggat
     1321 tacctttaat ttttaaaggt atttgtcagg catttaaaat cttaaaagag gaaaaaccaa
     1381 atattattat ctcctttggc ggctatgttt cagttccggt gattatcgcc ggctggttgc
     1441 ttaaaattcc ttcaattacc catgaacaaa ctcctactct tagcttagcg accagaatta
     1501 attctttctt tgtcaaccaa atcgctcttt ccttccctca tcccggcctt aagaaggctg
     1561 tagttaccgg taatcttctt cgccgggaac taacgaacca tcataaaaaa ctttttttga
     1621 cccaaaataa aactcttatc tatgttactg ccggcaatca aggatcacat tttcttaaca
     1681 acttaatcaa aataacccta ccaaaactaa atgacttcta tattatccat caaacaggaa
     1741 atattgatta tcatgaatat aaaaaattgg aaagacaata ttccaattat cgagtttata
     1801 gctatctgtc ggattcaatt gtagggtcag ttttctacca agcagaaatt attatcagcc
     1861 gagcgggagc taataccagc caggagattg tggctcttaa gaaaaaatct attttaattc
     1921 cccttccttt ctctcaacaa aatgaacaat tacttaatgc ccaatttgtt cgacagcaac
     1981 tgcctgatcg gacaattatt attcctcaaa gcgaaatcaa tcctgccatt ttagtaaaaa
     2041 ccataaaatc tctccttcct atcgcccctt ataaactaaa accaattaaa ccaaatttaa
     2101 aacttttgaa attaattcaa tcactttgca aaaagcggtt aaaatcgcta gaatgaaacc
     2161 agttttatgc agcactcaaa aataatcagt ggtatcgata tcggtagttc aaagatcacc
     2221 acgattattg gtcagtattt tgaacaggaa aatcgtttca atgttattgc cgtagcttct
     2281 tcaccggctg ttggttttag aaaaggacaa attatcaacc tagaacaagc agccgtaact
     2341 ctaactcaaa gtgtagaatc ggccgaaagg atggctggtc ttcaaattaa taatgcttat
     2401 gttgcctcag ccgccgctca tattgaatct tttaataaca aaggggtggt tgctgtcaat
     2461 aatcaaaacg gagaaattgc cgtcgaagat atccatcgag tcatcgaagc tactcaaaca
     2521 atctctctac cgactggaaa agaaatcctt catattatcc ctcgtgatta taccgtcgac
     2581 ggccaggaag gtattattga tccgattggg atgacgggat ctcgtttaga attggaggct
     2641 catttaatct tagcttcaac cccagctatt aagaatatta aaaaatgtct catggatatc
     2701 ggcatctcta tcaacgatct gatttattcc ggtttagcgg cggcgaaaag tgccttaacc
     2761 gatactgaaa aagaattggg gggggcctta gttgatatcg gcggcagtat tactacctta
     2821 accttatttt acgaatcttc accggctctt tcggcagtta ttcctatcgg ggctaataat
     2881 atcaccaatg acattgctat tggtttgaga ttttctcttg aaaatgccga aaagattaaa
     2941 gtccagctgg gtaaggttat ggagaacaaa aaatttgagg atgaaattga gttagcccat
     3001 tttggcgtca ttaatgaaag tcaaaaaaaa ctttctcttc aaaccactat taatggaatt
     3061 attaaacctc gtttagagga aattttttct attattctag gtaaaattca aaatagcggt
     3121 tactataatc tcattcctgc aggtattgtt cttaccggtg gtggagcttt aactgtcaac
     3181 gctaaagaaa tctgcagtaa aattatcggt ttaccggtac gtttatctca accgccatca
     3241 attggcggca tggttgatga tgttttgagt ccagcttata ccagtgcggt aggtttgctt
     3301 ttctattttc ttgacgataa gcgtaaattt aattttccaa ctatcggcag aagtcatcat
     3361 tctttcagca atgtttttag taaaataaaa agtttaatca aacccttgtt accttaattt
     3421 ttatgccttt aattaaaacc ctagctggac aattcgccaa gattaaggtt atgggaatcg
     3481 gtggtgccgg caataatgct attaattcga tgatcgagga agatatcatc catggggtgg
     3541 attttatcgc tgtcaatact gattctcagg ctctccttaa ctctaaagct atggttaaac
     3601 tccaaattgg agaaaaatta accaaaggtt tgggagctgg cggcaatcct aaaattgggg
     3661 aagatgctgc ccaagaatca caagaacgaa taaaagaatt actcgaagga acaaatttac
     3721 tttttatcac cggaggtatg gggggaggaa cctgtactgg agcggcaccg gttattgccc
     3781 aaattgccaa aaaagaactg ggtattttaa ctattgctgt ggttactaaa ccttttcttt
     3841 ttgaaggggc gcggcgttta gccaatgccg aggaaggtat ttctctgctt aaggacaaag
     3901 tcgatacttt aattgttatt cccaatcaaa aagtaatgga ggttattgat aatcaggcta
     3961 ccttattaga ggcctttaaa gtagctgatt caattcttaa taaaggcact cgggcgattg
     4021 ccgatttaat tactgttcct ggtttgatta atctggattt tgctgatatc aaatcaatta
     4081 tgagtgatgc tggctcagct ttaatggggg taggggaggc tgaaggagaa aatcgggtta
     4141 ccgcagccgt cgaagcagcc attaattccc ctttaattga agttaatatc gatggtgcta
     4201 gaggtgtttt aattaatatc accggcggac ccgatctaac tatgaccgaa gtcgaaaccg
     4261 ctgctaaggc tattaccgcc agaacttctc ccgacgctaa tattgttttt ggatacacta
     4321 ttgattcaaa tcttaaaggc aaaatgaaaa ttaccgttat tgctaccggc ttcgatacca
     4381 acacccagag gatttatcaa aattctaccc caacaactaa ccaatcttca ttggaaataa
     4441 atcaagaaac tattgataaa tacctaggta atcaagaaat tcctaaaggg atcgatatta
     4501 atgacgaatt tgatattccg gcgtttttaa gaaaggtgaa ataaaccaag aaaacaacaa
     4561 ttatagaaat ttgctaaaat tagccattat gtcctcattt aaatcattgc cggctaatct
     4621 cgattttccc gctttagaag aagaaatgct ccagtggtgg cagaaacacg acaccgtcag
     4681 ccaatatctt caccggaatg atcaatcaaa aaaaaggttt tcttttatcg acggtccgat
     4741 taccgccaat gggccgatgg gcgttcatca tgccagaggg cgaaccatca aagatgtttt
     4801 tcaacgttat aaaaacgccc agggattcgc tcaaagattt caaaatggat ttgattgtca
     4861 aggcttatgg gtcgaagttg aagttgaaaa agaaaatggt tttaattgta aaaaggatat
     4921 taaagaattt ggtctggcta aatttactca aagctgtatt gatcgagtca aaaattatgc
     4981 cgcccttcaa actaaacaaa gccaaagatt gggtatgttt atggactggg ataactccta
     5041 ttatactttt tctgaaacta ataatcttta tatttggtat ttcttaaaaa aaattaaaga
     5101 aaaaggttgg ctttaccgaa aaaaggcagc tactacctgg tgcccccgtt gtgaaactgg
     5161 cctatctcaa cacgaacaag ccgatagtta tcaaaccgtc aaggaaactt cagtctatct
     5221 taagtttaaa ataaaaaata aaaccgacga atatatcctc gcttggacaa ccacaccatg
     5281 gacactgcct gctaacgttc tcctagctat taaccccaaa tatgattatg ttaaagccaa
     5341 atataaaggg gaaaagatat atttagccaa aaaagcggcc gatagattga aattaaccga
     5401 ttatgaaatt atcaaggccc atcaattatt gggcgttgaa tatgaatctc tttatcatat
     5461 tccagcccaa gaaggaatcc atcatgccgt ggtccaatgg gatgcggttg atccggccga
     5521 cggcaccggc attgttcata ttgctcccgg ttgtggtcaa gaggactatg agttaggcct
     5581 atctttaggg gccgatataa tctcacctat tgatgaaact ggtcatttta tatccg
//
LOCUS       DAOOIE010000018         5621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_11602_length_5621_cov_2.920769, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000018 DAOOIE010000000
VERSION     DAOOIE010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5621
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11602_length_5621_cov_2.920769"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /gene="glyA"
                     /locus_tag="PK131_00965"
     CDS             <1..378
                     /gene="glyA"
                     /locus_tag="PK131_00965"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF012677.2"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPD44736.1"
                     /translation="NIIGAMAVTFAEAMKPAFKQYARQVVSNAAILAKTLKDLGWKVY
                     GTENHLMLVEVGLGKGKAAAVALEKADIFCNANTVPGEQGSPLEPSGIRLGSPAETTK
                     GKKEADFVKIGQKIDQILRANLM"
     gene            complement(393..2945)
                     /locus_tag="PK131_00970"
     CDS             complement(393..2945)
                     /locus_tag="PK131_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44737.1"
                     /translation="MSFIKRHIYPLILIILCLIICRLNYQSGTFLTGWDTLHPEFNFP
                     LNFSRLFNGVWRTEQGLGAIAGHSHMADLPRVILLWLFHFILPLNFLRYAYIFFCFVV
                     GPLGIFYLINYLFKNKLAAFTTAVFYIFNLGTVQQFYTPFEMFPTQWAFLPFIILSSL
                     KYLKENNQKNLFYFSLITLLSSPQAYAAHLWYAFFAVYTIFLMSHYFFYRNKKIFKLF
                     FFTLALNAFWLLPNLYYLFSSSSIPQNTKVNRLYSQEYLLQNRTTGYLRDAALIKGFY
                     FNWQVYNPEQAKFEPLMAAWNQHLAQPLIPFIGYLLFFLSLAGLIISFVKKNKILISL
                     SPFWLIPFILLMNHTPPFSYLFNFLIKYTLFQEAFRFIFTKISIMFTFGIALYLAYFL
                     NIFKLKVLNLPIILASLIFAFPVFRSQLISPKLRVKIPDEYFQFWQYVNTLPQGKTLP
                     LPLHNFAGWQYYNWGYQGAGFIWFNLKQPILDRDFDRWAPQNEEAYREFFYSLYRRDP
                     AVLAATLQKYHFKYILWDKSIITPYRKNREQIVFENEILNLLNQLEKEKIISRDKNFG
                     FLSLYRVDSPDWIKPKYVTNYVKEPYLWSYWDPIFLNQGNYYTDSSDYFNHNQLLDKN
                     ERVNYQNILPYLDLDQVKNYFPNVKHYKTFNRSTGNFLVIPELAHNQAYLVGFNSRYL
                     TGLPLRICLKNTYNNICTVYEQLSKNKNFAWDYYLIPPQDNFFGFEITVDNISLGSYP
                     TENELAEIRIIPVDFDKLTQNTNIIAQPRLIISNLQSYHPGWIAFYFNGFKPVRLKNH
                     LLVNNWANGWVVDRLPDGRVYYLFWPQLLEYLGFIFAISILIHDLRQRKTPA"
     gene            complement(4022..5065)
                     /locus_tag="PK131_00975"
     CDS             complement(4022..5065)
                     /locus_tag="PK131_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44738.1"
                     /translation="MNKETQKTIATVITTAIISGGAGYGIAKIWGEKPEIPTPTPTPV
                     TAEMTPEKNQVQSEIKAKEEDEKIEQINFNEGQSSYDLSVKAGTINKIWSEKGLTVRG
                     TQFKTNEDTILWIIGDNCEDAVVGLENLPKSGTKLVAFKDQDVKNYKDFYNKYFNELI
                     PDKTGIQNSIVIAIENQAKVRFSNIGKKEVKKEEPKVVERIIERTIIVRETPKPEPKK
                     PEVKKPEVKKPEVAKQPEKKPERISIQVNPNVEWEAQPGWIVSGDIEYFDSATNTWQR
                     VYDTWQDYPEGKDKSARTADIVVIQNRVKIRGEWGFTRSFNIPLAEIKANKENEKDKN
                     GSKIFDEVRVKYL"
     gene            complement(5231..>5621)
                     /locus_tag="PK131_00980"
     CDS             complement(5231..>5621)
                     /locus_tag="PK131_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44739.1"
                     /translation="ALEWIDGSRYASQHPCLDFEIYNCDPTKIVKVKVNCPFRYEDRK
                     YSKWYYFDKNTILLLTPGRYFFPCGYTELYEKIILLPTEDPIKRCEVEVYNLDRIVFI
                     PQAILKYNTVYETYFENSLLFHSSPGW"
BASE COUNT         1766 a          958 c         1284 g         1613 t
ORIGIN      
        1 aatattattg gtgccatggc tgtcactttt gcggaggcaa tgaaaccggc ttttaaacaa
       61 tatgcccggc aggtagttag taatgccgct attttggcta agactttgaa agatttgggt
      121 tggaaggttt atggaacgga aaatcatcta atgctggtcg aagtcggatt agggaaaggg
      181 aaagcggcag cagtcgcctt ggaaaaagca gacatctttt gcaatgccaa tactgtgcct
      241 ggcgagcagg gaagtcccct agagccatca ggcatcagat taggttcacc ggcagaaaca
      301 accaaaggca agaaagaggc ggattttgtc aaaatcggcc agaaaatcga ccaaattttg
      361 agagcaaatt taatgtaggg gttgaccgag tgtcaagctg gtgttttccg ttgccttaaa
      421 tcatgaataa gtattgatat ggcaaaaata aagccgaggt attcaagtaa ttgaggccaa
      481 aagagataat aaacccgccc gtccggcagg cgatcgacaa cccaaccatt agcccaattg
      541 ttaaccagaa gatggttttt taaccgaact ggtttgaagc cgttgaaata aaaagcaatc
      601 caacccggat gatacgattg gagattggaa ataattaaac ggggttgagc aataatgttg
      661 gtattttggg ttaatttatc gaaatcaacg gggataattc tgatttccgc caactcattt
      721 tcggtagggt aggagccgag ggagatgttg tcgacggtaa tttcaaagcc gaaaaagttg
      781 tcctggggcg ggattaaata ataatcccaa gcgaagtttt tgtttttgct tagctgttcg
      841 taaacggtgc agatgttatt gtaggtgttt ttgaggcaaa tgcggagagg caggccggta
      901 agatagcggc tgttaaagcc gactaaatag gcttggttat gggctaattc ggggataacg
      961 agaaaatttc cggtagatcg gttaaaagtt ttgtaatgct taacattagg aaagtaattt
     1021 ttgacctggt ctaaatcaag gtaaggaagg atgttttgat aatttaccct ttcgttttta
     1081 tctaataact gattatgatt gaaataatca gacgaatcgg tgtagtaatt tccctgatta
     1141 agaaaaattg gatcccaata ggaccaaaga tatggttctt ttacataatt agttacatat
     1201 tttggtttaa tccaatcggg agaatcaact cgataaaggc ttaaaaagcc aaaattttta
     1261 tcccggctga taattttttc tttttccaac tgatttaaaa ggttgaggat ttcattttcg
     1321 aagacaattt gctcccggtt tttgcgatag ggagtaataa tcgatttgtc ccaaagaatg
     1381 tatttaaagt gatatttttg caaagtggcg gctaaaacgg ccggatcccg acggtaaagt
     1441 gaatagaaaa attcccgata ggcttcctcg ttttgaggtg cccagcggtc aaaatcgcgg
     1501 tctaaaatcg gttgtttaag attaaaccaa ataaaaccag caccttgata gccccagtta
     1561 taatactgcc agccggcgaa attatgcaga ggtaacggca gagtttttcc ttgaggcaaa
     1621 gtattcacat attgccagaa ttgaaaatat tcatcaggaa tttttacccg taatttagga
     1681 gaaattaatt ggctacgaaa aacggggaaa gcgaatatta atgaagctaa aatgatgggt
     1741 aaatttaaaa cttttagttt aaaaatgttg agaaaataag ctaaataaag ggcaattccg
     1801 aaagtgaaca tgatggaaat tttagtgaaa ataaaacgaa atgcctcctg aaagagggta
     1861 tatttgatta agaaattaaa aaggtaagag aagggtggcg tatgattcat cagaaggatg
     1921 aaggggatca gccaaaaagg agaaagagaa ataagaattt tatttttctt gacaaaactg
     1981 atgattaaac cggccaagga gagaaagaac agcagatagc caataaatgg gattaagggc
     2041 tgggccaaat gctggttcca agcggccatt aacggctcga atttagcttg ttctggatta
     2101 taaacctgcc aattaaaata aaaaccttta atcagagccg catcccggag gtaaccagtg
     2161 gtccggtttt gcaggaggta ttcttgagaa taaaggcggt tgactttagt attttggggg
     2221 atggaactgc tggaaaaaag atagtaaaga ttgggtaaga gccaaaaggc gttaagagcg
     2281 agagtaaaaa agaataattt aaagattttt ttgtttcgat agaaaaaata atggctcatt
     2341 aaaaagatgg tataaacggc aaaaaaggca taccaaagat gagcagcgta ggcttgaggg
     2401 ctggaaagga gagtgataag ggaaaaatag aataaatttt tttgattatt ttctttaaga
     2461 tatttgaggg aagataagat gataaacggc agaaaagccc attgggtggg gaacatttcg
     2521 aagggagtat aaaattgttg aaccgtgccg agattaaaaa tataaaaaac ggcggtggta
     2581 aaagcggcca gtttattttt aaaaagataa ttaataaggt aaaaaatacc tagcggtccg
     2641 acgacaaagc aaaaaaagat ataggcataa cggagaaaat taaggggcaa aataaaatga
     2701 aaaagccaaa gtaaaatgac ccggggtaag tcggccatgt gggaatggcc ggcaatggcg
     2761 ccgagacctt gttcagtccg ccagacaccg ttaaacagtc gggaaaagtt taaaggaaaa
     2821 ttgaattctg gatgaagggt atcccaacca gttagaaagg tgccggattg gtaattaagc
     2881 cggcagataa tcaggcagag gattattaaa ataagcgggt agatgtgcct tttgatgaaa
     2941 gacatgatta agtatagcgc aaaatccccc tgtcgcttca tcggcgacat ctccctttga
     3001 taaaaggggg attaaaaagg aaaaaatgta gaagtgggga tttaaaattt aaataggaaa
     3061 ttgaaaaaga aagattgtag agagaaatga aagaagtgaa atgaaggttg aatggagtta
     3121 aagaggagag aagggtgata agcggggcgt aaattcgtcc ggctatcatg cataccttct
     3181 ctcacgagtt gtggggttta tggccaagtg gtataaaaac cattcgggta ttgggagcgg
     3241 atttgggaat tggtagtgcc acgaaacaat aaactgccag tatttgcggt ataaactgtt
     3301 ccggattgta agatctggat taccgtgtat ggatatgaag gcgtgatttg ggaagcgttt
     3361 ccacaagaat tctgtgtaaa gtatgctgta ctgtatatta ctaaccaatc cccatacacg
     3421 atcggggttt gatagtatgc cgaagtgctg attggggttg attggtatcc tgagccggtt
     3481 ggccacgatg ttgtagtcat gtcccgtgtc catccggggg atctattccg aatagactcc
     3541 tccacctgtt gctcgcaggt gcccgtccag accgtggcgc cccatgggaa ttcaaggtaa
     3601 ctgcgttggg taacagtaaa aaacaagtag gttgtgctac agttgtcgca caattgtgtg
     3661 acacggttcc cacacgccgg gtctaaagaa aagcagtccc cgtccacgat ctggccgggt
     3721 tcgacccaga tctttccaca aacatacatt gagctggcct gagcggagat agggaggaat
     3781 aaaacagcag cgagcatcga aaaaacaact aaaaaaacca cacaaaatcg tttcatttta
     3841 aactcctttc ttttatttat ttttctggta ctaaagtacc catctagata tgacttataa
     3901 ttcataccca catgggtacc tagtaccagc cgattctctc tacaatctgt taaagtacat
     3961 cccttacctg atcgcctttg atcagttagg taagagtatt atagcactta gatgggattt
     4021 tttacagata ttttactcgg acctcatcaa aaattttgct gccgttctta tctttttcat
     4081 tttctttatt agctttaatt tctgctaaag gtatattaaa actgcgggta aagccccatt
     4141 cgccgcgaat tttaacccga ttctgaatta cgacgatatc agcggttctg gcagatttat
     4201 cctttccttc gggataatct tgccaagtat cataaactct ttgccaagta ttagtcgccg
     4261 agtcaaaata ttcaatatca ccagaaacaa tccagcctgg ctgagcctcc cattcaacgt
     4321 tagggttgac ttggatactg attctttccg gtttcttttc cggttgtttg gcaacttccg
     4381 gtttttttac ttccggtttt ttaacctctg gttttttagg ctccggttta ggagtttccc
     4441 gaacaataat agttctttca ataatcctct ccactacctt cggctcttct ttcttaactt
     4501 cctttttgcc gatattggaa aatctaacct tggcctgatt ttcaatcgct atgacaatac
     4561 tattttgaat gccggttttg tcgggaatta gttcattgaa atatttatta taaaaatctt
     4621 tgtaattttt aacatcttgg tctttgaaag caacaagttt ggtaccagat ttaggaaggt
     4681 tttctaagcc gacgacagcg tcttcacagt tatcgccgat aatccacaga atcgtatctt
     4741 cattagtttt aaattgggtg cccctaacag tcagaccttt ttcactccaa attttgttga
     4801 tagtgccggc tttgacggat aaatcgtaag acgattggcc ttcgttgaaa ttaatttgtt
     4861 caattttctc atcttcttcc ttagctttta tttcagattg aacttgattt ttttccggag
     4921 tcatttcggc ggtgactggg gtaggggttg gggtaggaat ttctggtttt tctccccaga
     4981 ttttagctat tccataacca gcaccaccac taattattgc cgtggttatt acagtggcga
     5041 ttgttttttg agtttcttta ttcatatata attatattat atcatactag aacaaaaagg
     5101 tcaagcggaa agaagaaggg ggacagaagc gcaggggcag caataaacaa ataaaataaa
     5161 atttgtttaa agcatacgct tctgtcctgt aaaatcaggg ttaatggttg ttaaaaaatt
     5221 aaaaaaacta ctaccaaccc gggctggagt gaaacaataa ggaattttca aagtaggttt
     5281 catacacagt attgtatttg agaatggctt gaggtataaa aacaattctg tccaagttgt
     5341 agacctcaac ctcacaccgt ttgattgggt cttcggttgg gagcagtata attttttcgt
     5401 acagttcggt atagccacac ggaaagaaat agcggcctgg agttagcaac agtattgtgt
     5461 ttttatcaaa ataataccac ttgctatatt tcctgtcctc atacctgaaa ggacaattta
     5521 ccttaacttt tactattttg gtgggatcac agttgtatat ctcgaagtcc agacacgggt
     5581 gttgactggc gtatctggaa ccatcgatcc attccagtgc g
//
LOCUS       DAOOIE010000019         5522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_11853_length_5522_cov_11.546369, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000019 DAOOIE010000000
VERSION     DAOOIE010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11853_length_5522_cov_11.546369"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PK131_00985"
     CDS             complement(<1..481)
                     /locus_tag="PK131_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44740.1"
                     /translation="MPEQIGLKSSFYMPKHTHADNNNGGTLFGNDAVKANSINLGLIA
                     KDTIGYGDEGNNNFKFFEKTFSGVKTSNTTYTSLWTFTARANIYFTIEFNVVSDNIAY
                     ACIRDGTTIISPEYTFDAGGLIQKFTKTTYYEMGKTYNIALKSEDPAFYAHLLFVKFY
                     "
     gene            complement(483..827)
                     /locus_tag="PK131_00990"
     CDS             complement(483..827)
                     /locus_tag="PK131_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44741.1"
                     /translation="MLYDFEASKNVAAGATETLFSFQVPENNILVFRKFGNDIDNMTA
                     WGSVTFSINKNGIGVYPYENIKDQLGFLAIPKDISQIKLNPFDTLTIKVKNDYGSTIK
                     AGIVFQYDLLEV"
     gene            complement(830..1342)
                     /locus_tag="PK131_00995"
     CDS             complement(830..1342)
                     /locus_tag="PK131_00995"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPD44742.1"
                     /translation="MYLILILVTLAGLIFLATKHNAEDKKLIENAKKEIDKLYAITAP
                     SMTLDELIKYKAGKYGIEERLLRAIIKVESDFNPRAKNLKDPSYGLCQINPALAKGLG
                     FFPYEYLLYDPAYNLEIACVFLSHLKRKYNNDLDSIIQAYNLGETKFDKGYKSPDYLE
                     KVKKYYYQGI"
     gene            complement(1353..1556)
                     /locus_tag="PK131_01000"
     CDS             complement(1353..1556)
                     /locus_tag="PK131_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44743.1"
                     /translation="MKKYFKPLLLLGVLLAMFFYLYKKNKKQEIIVTQTADLPPPNNQ
                     IMDWFDNYFKFGTTDTQKKLAAL"
     gene            complement(1540..1680)
                     /locus_tag="PK131_01005"
     CDS             complement(1540..1680)
                     /locus_tag="PK131_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44744.1"
                     /translation="MAEFFNFLSLLSLLIIPVLKYVMDIEKRLTRLETLIQGMKNNEK
                     VF"
     gene            complement(1681..2118)
                     /locus_tag="PK131_01010"
     CDS             complement(1681..2118)
                     /locus_tag="PK131_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44745.1"
                     /translation="MNILDSLNSAWTSLMNNLSDIYYRSKYGLDISPEDDLWFDQITN
                     VTAPAVEKVVSLDSLNSAWTSLMNNLSDIYYRSKYGLDISPEDDLWFDQITNVTAPAV
                     EKVVSSGNKVKEALLSIPRAVKYILIGAGVLAIIFLISNIRKK"
     gene            complement(2115..2264)
                     /locus_tag="PK131_01015"
     CDS             complement(2115..2264)
                     /locus_tag="PK131_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44746.1"
                     /translation="MSKPILQSVTRKIEPVKTSTGQTTGLTSVNNLLAIGAILALLII
                     GALFK"
     gene            complement(2261..2473)
                     /locus_tag="PK131_01020"
     CDS             complement(2261..2473)
                     /locus_tag="PK131_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44747.1"
                     /translation="MTSIDLGAWANDLFKIGTDYLSSKLVTPNPTQPVVVYQPPPVET
                     TSSSDNILKIGLLAAAGVAVYMLVKK"
     gene            complement(2470..2694)
                     /locus_tag="PK131_01025"
     CDS             complement(2470..2694)
                     /locus_tag="PK131_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44748.1"
                     /translation="MVAAIVSGIGNLIGGIFGGNKAKKAARQASDQIKKYVADLEAKV
                     KIAFGDYGKVFYLVFILIFLLFILIIRGKK"
     gene            complement(2699..3655)
                     /locus_tag="PK131_01030"
     CDS             complement(2699..3655)
                     /locus_tag="PK131_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44749.1"
                     /translation="MRTPFERRLIFSAKPLSNSAVNTFRTELPVTGENLERIRLILNL
                     TITHGTGTTPVTTGAFDYIKAITLKTSKGDLLVDGVCGKALYFYNLKNDQVTPYYDDI
                     AASSGTYKAVIDIPFHRNNLIRPEDLYYLTNYASLELSVTTGSIADLLGTPGTDSVSA
                     SLDVVYHLTKSPLSKSEKSFPLALPYYKQLATIPLTSLPYFQLESANDLALSEFLILA
                     TTSNTAPFRGTAADNITTLSMKDNVQYFIQQVPVADFIQERKRHFLSNLTGIYYHSFI
                     KTGSIKESYPTGGKSDIKITADTFTGTTYADCLLIGFRSFRI"
     gene            complement(3901..4155)
                     /locus_tag="PK131_01035"
     CDS             complement(3901..4155)
                     /locus_tag="PK131_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44750.1"
                     /translation="METITLTLVITEFFKKILSKINIKVEGVLAWILSGLVAFFVCLY
                     YHIDHFNLAEFIVIFIQTLLGANAGYITLNNLAKTAGGKN"
     gene            complement(4202..4684)
                     /locus_tag="PK131_01040"
     CDS             complement(4202..4684)
                     /locus_tag="PK131_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD44751.1"
                     /translation="MLISIIGKRGTGKTTLAKQIIKAQSWDNLFIYDYLGEYKEFKNE
                     ATYSRTNLYQVLKLSWNASNPNENNLLVLDEVDLYGYGNEQLEFLYRYGRHKNLSIIA
                     ISRRFYNLPVYIRALTEVFYLFKITEERDLNYLKSLVSKAFISKIITLEKYHYIKLQL
                     "
     gene            complement(4602..5018)
                     /locus_tag="PK131_01045"
     CDS             complement(4602..5018)
                     /locus_tag="PK131_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44752.1"
                     /translation="MYKVNDDLPEFPESNEDEEEKQDGSFSSIENLEELCGDIAAVPF
                     EIWSILNPKVEKLTDTEKKAISRPLARLVEKYNLGTYAKDEFYLAIILTGVTFKRINQ
                     AKPEKKKKDNANINNRETGNGKDDLSQADNKSSELG"
     gene            complement(4930..5217)
                     /locus_tag="PK131_01050"
     CDS             complement(4930..5217)
                     /locus_tag="PK131_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44753.1"
                     /translation="MKEPKTEDLNLEGTNLVEEPIEGNQDTNPAPVVNTTIDLSQSDF
                     IPEKKKRRRRKKIEIENERKENNVQGQRRLTGVSGKQRRRRGKTGRLFFFD"
     gene            complement(5239..5313)
                     /locus_tag="PK131_01055"
     tRNA            complement(5239..5313)
                     /locus_tag="PK131_01055"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5279..5281),aa:Met,seq:cat)
     gene            complement(5304..>5522)
                     /locus_tag="PK131_01060"
     CDS             complement(5304..>5522)
                     /locus_tag="PK131_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44754.1"
                     /translation="DDLIFLIAFLWKWSDESDALTALTHEEEYEGEAYESFSRLVRET
                     LKEDDFYRARKIFWSLKPEITKISGDAE"
BASE COUNT         1667 a         1015 c          958 g         1882 t
ORIGIN      
        1 cataaaattt aacaaataat aaatgagcat agaaagcagg atcctcactt tttaaggcaa
       61 tattatatgt tttacccatt tcataatagg ttgtcttagt gaatttttgt attagtccgc
      121 cagcgtcaaa ggtatattct ggacttataa tagttgttcc gtctctaata caggcgtagg
      181 ctatattatc cgatacaacg ttaaactcta tcgtaaaata aatattagct ctggctgtaa
      241 aagtccaaag tgaagtgtaa gttgtatttg aagttttaac tccagaaaaa gttttttcga
      301 aaaatttaaa attattgttc ccttcatcgc catatccgat agtatctttc gctattagtc
      361 caaggttaat tgaattggct ttaacggcat cgttcccaaa tagtgttccc ccattattat
      421 tgtcagcgtg tgtatgcttt ggcatataaa aagagctttt taaacctatt tgttctggca
      481 tattaaacct ctaaaagatc gtattgaaaa acaattcccg ccttgattgt tgaaccataa
      541 tcatttttta ctttaattgt caaagtatca aagggattaa gttttatttg agagatgtct
      601 ttcgggatag ctaaaaatcc aagctgatcc ttaatgtttt cgtaaggata aactccgatc
      661 ccatttttat ttattgaaaa ggtaaccgat ccccaagcgg tcatattatc gatatcgttt
      721 ccgaatttcc ggaaaactaa gatattattt tctggcacct gaaaagaaaa gagtgtttcc
      781 gtagctccgg cggcgacatt ttttgaggct tcaaaatcat aaagcatttt tatatcccct
      841 gataataata tttttttacc ttttctaaat aatctggcga tttatatccc ttatcaaact
      901 ttgtctctcc cagattatac gcttgaataa tagagtcaag gtcgttattg tatttccgtt
      961 ttagatgtga caagaaaacg caagctattt ccagattata ggctggatcg tataagagat
     1021 attcgtaagg aaaaaatcct aatcccttag ccaaagccgg attgatctgg cagagtccat
     1081 aagacggatc ttttagattt ttcgcccgag gattaaagtc gctctctacc ttaataatcg
     1141 ccctcagcaa tcgctcttct attccgtatt ttccggcctt atatttgatc agctcgtcta
     1201 aggtcatcga tggtgccgtg atagcataga gtttgtcgat ttccttttta gcattttcaa
     1261 tcaatttttt atcctcagca ttatgttttg tcgctaaaaa aattagtccg gccagagtaa
     1321 ctaaaatcaa tattaaatac atagctaaac ctttataaag ccgcaagttt tttctgagta
     1381 tctgtagttc caaacttaaa ataattatcg aaccagtcca ttatttgatt attaggcggt
     1441 ggtagatcgg cggtctgtgt tactataatt tcctgttttt tgttcttttt atataggtag
     1501 aaaaacattg ctagtaaaac acctaaaagc agtaaaggtt taaaatactt tttcattatt
     1561 tttcattcct tgtattaaag tttcaagcct ggtcaagcgt ttttcaatgt ccataacata
     1621 ttttaaaaca gggataatca gtaaagatag caagcttaaa aaattaaaaa attcagccat
     1681 tcattttttc cttatattag aaataagaaa aataattgct aaaactccag cgccgattaa
     1741 aatatatttt acagctcttg gtattgatag tagagcttct ttaactttat tccccgatga
     1801 aaccactttt tcaacagccg gtgctgtaac attagttatt tgatcaaacc agagatcatc
     1861 ttctggtgat atatcaagtc catattttga tcgataatat atatcggata aattattcat
     1921 tagtgaagtc caagcgctat ttaagctgtc taatgaaacc actttttcaa cagccggtgc
     1981 tgtaacatta gttatttgat caaaccagag atcatcttct ggtgatatat caagtccata
     2041 ttttgatcga taatatatat cggataaatt attcattagt gaagtccaag cgctatttaa
     2101 gctgtctaaa atattcattt aaacaaggct ccgattataa gtaaggctaa aatcgctccg
     2161 atagcaagta aattattaac agatgttaat ccggtagtct ggccggtaga agttttgacc
     2221 ggttcaattt tacgggttac actttgaagt ataggtttac tcatttttta accagcatat
     2281 aaactgctac tccagcggcg gccagcaatc cgatctttag aatgttatca gagcttgaag
     2341 tcgtttctac cggcggtggc tgatatacta ctaccggctg agtaggatta ggcgtaacaa
     2401 gttttgatga aagataatca gttccgatct taaataaatc gttagcccaa gctccaagat
     2461 caatacttgt cattttttcc ctctaataat caatataaaa agtaaaaaaa ttagaatgaa
     2521 taccagataa aaaacttttc cgtagtcacc aaacgctatt tttactttgg cttctagatc
     2581 ggctacgtat ttttttatct ggtcagaggc ctgccgagcc gcttttttag ctttatttcc
     2641 gccgaatata ccacctataa ggttcccgat cccgctaaca atagcagcaa ccatatcatt
     2701 atattctaaa gcttctaaag cctatcaaca ggcaatcggc ataagtggtg ccggtaaaag
     2761 tatcggcggt gattttaatg tcgctttttc cgccggtcgg gtaactctct ttaatcgatc
     2821 cggtcttaat aaagctgtga taataaatac ctgtaaggtt acttaggaaa tgtctttttc
     2881 tttcctgaat aaaatcagca acaggcacct gctgaataaa gtattgaaca ttgtctttca
     2941 ttgagagagt cgttatatta tccgcagcag tccctctaaa aggcgctgta tttgaagttg
     3001 tagcaaggat taaaaactca gacaaggcca gatcattagc ggactcaagc tgaaagtatg
     3061 gtaaagatgt taaaggaatt gtcgcaagct gtttgtagta aggcagagcc agaggaaaac
     3121 ttttttcaga tttagataaa ggcgatttag tcaagtgata aactacatcg agagacgctg
     3181 aaacgctgtc agttccagga gtccctaaga gatcagcgat tgatccggta gttactgaaa
     3241 gttccaaaga ggcatagtta gttagataat aaaggtcttc aggcctgatc agattatttc
     3301 ggtgaaaagg aatatcaatt actgccttat aagtcccaga actggcggct atgtcgtcat
     3361 agtaaggcgt tacctgatca tttttcagat tataaaaata taaagccttc ccgcaaactc
     3421 cgtctaccag cagatcacct ttagaagtct taagagtgat cgccttgata taatcaaaag
     3481 ctccggtagt tactggtgta gttccggttc cgtgtgttat agtgagatta aggattaggc
     3541 ggatcctctc cagattttca ccggtaacag gcaattcagt tctgaaagta ttaacagctg
     3601 aatttgacag cggcttagct gaaaaaatta accttcgttc aaaaggtgtc ctcatttgtt
     3661 acactccata taccaatttt tttagaaaac taactctgtt tgttagaaaa attacaacca
     3721 aacaaacagc ggcggtcatt aaaaaaggct taagggttct tttcattgat ttattctcct
     3781 aaaataaagt agatttttta cgccttttag gcgatttttt ccgtttttta gattttccta
     3841 aggggatcat aagatagcgt gatccgccaa tctttacctt tttaactctt tttctttttt
     3901 tcaattttta ccaccagcag ttttagctag attgttaagt gtgatatatc cggcgtttgc
     3961 tcccagcaag gtttgaataa aaatcactat aaactccgcc aagttaaaat ggtcgatatg
     4021 ataataaagg cagacaaaaa aagccaccag accggataaa atccaagcta aaactccctc
     4081 aactttaatg ttgattttag aaagtatttt tttaaaaaat tcggtgatta ccagagttaa
     4141 agttattgtt tccatattta aatgatcgta tttttaaagg ctaaaatcaa gcagtaaaaa
     4201 tctataattg aagttttata tagtgatatt tttcaagtgt tataatctta gagataaagg
     4261 cctttgaaac cagagattta agataattaa ggtctctttc ctcagtaatt ttaaagagat
     4321 aaaaaacctc agttaatgct ctgatataaa ccggcagatt ataaaatcga cggctaatag
     4381 caattattga taagttttta tgtctgccgt atcgatatag aaactctaat tgttcgtttc
     4441 cgtatccgta taaatcaact tcatctaaaa ctaaaagatt gttttcattc ggatttgaag
     4501 cgttccagga taattttaaa acttgataaa ggtttgttct tgaataggtg gcttcatttt
     4561 taaattcctt atattcgcct aaatagtcat aaataaatag attatcccag ctctgagctt
     4621 ttattatctg cttggctaag gtcgtctttc ccgttccccg tttcccgatt attgatatta
     4681 gcattgtctt ttttcttttt ttcaggctta gcttgattta tccgtttaaa ggttacacct
     4741 gttaaaatga tagcaagata aaattcgtct ttagcataag ttcctaaatt gtatttttca
     4801 actaagcgtg ctaaaggccg gctaatcgcc tttttttccg tatccgttag tttttctact
     4861 ttaggattaa ggatactcca gatttcaaaa gggacggcgg ctatgtctcc gcaaagttct
     4921 tctaaatttt caatcgaaga aaaagagccg tcctgttttt cctcttcgtc ttcgttgctt
     4981 tccggaaact ccggtaagtc gtcgttgacc ttgtacattg ttttctttcc tctcgttttc
     5041 aatttcgatt tttttccggc ggcgcctttt tttcttttca gggataaaat cagattgaga
     5101 tagatcaatt gtagtattaa caacgggagc cggattagta tcctgattgc cttcaatagg
     5161 ttcctcaaca aggtttgtcc cttcaaggtt taaatcctca gtttttggct ctttcatttt
     5221 taacctctat aattaaaatg gtagcggaga gagggatcga acctctaatt tccaggttat
     5281 gagcctggcg agttaccatt actctactcc gcatctccgc tgattttcgt tatctctggc
     5341 ttgagagacc aaaagatttt tctggctcga tagaagtcgt cttctttcaa tgtttctctt
     5401 actaggcggc taaaactttc gtatgcttca ccttcgtatt cttcttcatg tgttagagcc
     5461 gtcaacgcat cagattcatc agaccatttc cagaggaaag ctatcaagaa aataagatca
     5521 tc
//
LOCUS       DAOOIE010000020         5426 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_12106_length_5426_cov_2.798920, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000020 DAOOIE010000000
VERSION     DAOOIE010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5426
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12106_length_5426_cov_2.798920"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /locus_tag="PK131_01065"
     CDS             <1..1079
                     /locus_tag="PK131_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44755.1"
                     /translation="SITIAAMLYTYQGGKLMALILIFILLINQISNLTQLKEWLYRHF
                     FNFIFPLIILALPVIFGLLFKSESNRLRVASLFSYPRSAQEAQQIIAEGGQVDFNLFH
                     NQSIYFLRNSLLRYFNHFSPEFLTFVGDWQNPRHSSPYTGVLLLPSIIFLIIGLLNYR
                     KKDLFFLLWLLLAPLASALTRDSVSATRAMSFSIPLIYFTAAGILIFLKRFPYFTVYC
                     LLITVYLLSFIYWADLYFNHMTKTHPSDWLVGYKEAMVYIIQKQDQYQTVTFTDFYGQ
                     PYIYYLFYSKYPPAKYQQQAQLISSSPDVGQIKKIDKINFGPANFPPVNNSLTVFSYD
                     EIVRQNLDFKNFTRFGNFYAYENP"
     gene            1066..2574
                     /locus_tag="PK131_01070"
     CDS             1066..2574
                     /locus_tag="PK131_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44756.1"
                     /translation="MKTHKIFFILILAIVLRFLFIGTMPALNPDEAALGYNAYSLLKT
                     GKDEHGSSWPIHFKSFGDYKPGGYVYLALPFIKILGLTPLATRLPNLILSILTIYYFY
                     LLINQLSKNDNLAALSALILTISPWHLHFSRGAWEAGAALAFTVIGTYCFFKNQKYLF
                     ILFFSLSLYTYHSARLITPLLAIILIILNYKIEIRNWKKWLLPIVFGIIICLPVLFSF
                     LHQGGASRLNGVGLAADKGPLNRAEELLNQHGNTKIFNRIIHNKRVLYLLSWGQKYAS
                     HFNPNFLFINGDEVPRSKVPEMGLLYLIELPLLIYGFYESKKRNQSNLLMFSWLLIAP
                     IASSLTFQAPSALRSLPMVVPLSYFIAVGIINTQKVYPPEGGRAIYCLLIAIYLFSFA
                     YYLDAYYRHYQKRWPFAWNYGFAQIVPYVESQKNQYDHIFFTNKFDQPYILYLFFSQY
                     PPAQLQPQIKLTPPDQYGFSTVDQIDNITFHIPPEKDIPPHSLVISDKMLLP"
     gene            2571..3380
                     /locus_tag="PK131_01075"
     CDS             2571..3380
                     /locus_tag="PK131_01075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPD44757.1"
                     /translation="MNLSVTVATYNEEANIGHFLNNVSDIADEIIIVDGKSTDKTVKI
                     AREFARRQTTTKIKIIITDNKPMFHFNKQMGIDACQGNWILQLDADERLTHELKKEIL
                     EIAGKDPQTVEFNGYWLKRKNYFLGSFLKKGGQYPDPTLRFYKRGRGHLPCRDVHEQA
                     VVNGKVGWLKHDLEHYADPSFSRYLLRANRYTTLLAQQLVGQKVPINFLSFVKYYFVL
                     PQGWFLKTFFRHRGYVDGFRGFVFSFFSALRFPIAYTKYFELKRNISQGWE"
     gene            3383..4399
                     /locus_tag="PK131_01080"
     CDS             3383..4399
                     /locus_tag="PK131_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPD44758.1"
                     /translation="MISKIKIGFVSSPLNNQNKVRGVGYYTKKLYENLSKIKTLEVNL
                     IKNWNLKIENYDLIHYPFFDPFNLTLKIFKQPTVVTVHDLIPLQFKKHFPAGFKGNLK
                     WLIQLYHLRRAAAIITVSQYSKKIIHQITHIPLNKIFVTYLAADSTFKPINNKSYLQA
                     IKAKYHLPDKFVLFVGDINWNKNIPSLVKICQHLNYPLVIAGSAAVQKNVPIHPWTKD
                     LLWLQQQKYPLKLGFVPETELPALFNLATIYCQPSYAEGFGLPPLQAMACGCPVICRM
                     ATSLPEVVGSAALPFSIDSFQKFWQEKKIREKYIKLGLDQAKNFSWEKTARATMEVYV
                     KINS"
     gene            4380..5135
                     /locus_tag="PK131_01085"
     CDS             4380..5135
                     /locus_tag="PK131_01085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD44759.1"
                     /translation="MSKLTPNHFLSLIVPVYKQEKTIKKDIVRLLKTLDQIRYPSELI
                     AVVDGILVDTSYYILKKIKNPKLKVIAIKRNQGKGYAIRKGMAKAKGDYIAFIDAGME
                     IDPNGISMILEHMEWYNADIIVGSKRHPASIIQYPLDRKIISFCAQIYARLFLGLKIT
                     DTQAGLKIFKRRVLEKILPKLLVKQYAFDLEILAVANRLGFKRIYEAPIKLNYSFTSL
                     THANSLNTIYKTLIDSLAVFYRLKILHYYDKKN"
     gene            5119..>5426
                     /locus_tag="PK131_01090"
     CDS             5119..>5426
                     /locus_tag="PK131_01090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HPD44760.1"
                     /translation="MTKKIDFSVIIPVRAPNSYLKETLAHLKQQIYKQFEVLVITDNV
                     SKTPNPAAKRNLGAKMAKGKYLAFLDDDSYPDRFWLKNAKKQLEKKSIIAAVGGPCLT
                     P"
BASE COUNT         1581 a         1215 c          817 g         1813 t
ORIGIN      
        1 catcaattac cattgccgcc atgctttaca cttatcaagg cggcaaatta atggctttaa
       61 ttctaatttt catcctatta atcaaccaaa tttcaaatct aactcaatta aaagaatggc
      121 tttatcgcca ttttttcaat tttatttttc ctttaattat tttggctttg ccggtcatct
      181 ttggtctcct ttttaaatct gagtcaaacc gtcttcgggt ggccagcctg ttttcctatc
      241 cccgatcggc ccaagaagcc caacagatta tcgccgaagg cgggcaagta gattttaatc
      301 tttttcataa tcaatctatt tactttttaa gaaattcact tcttcgttac tttaatcatt
      361 tttcgcctga atttcttact tttgttggtg attggcagaa tccgcgccat agctctccct
      421 acaccggagt tttacttctg cctagtatta tttttcttat tatcggcctt ttaaattaca
      481 ggaaaaaaga cctatttttt cttctttggc tcctcttggc ccccttggct tcagccctaa
      541 cccgcgacag cgtttctgcc acccgggcca tgtccttctc tattcctcta atttatttca
      601 ctgccgctgg catcttaatt ttcctaaaaa gatttcctta cttcactgtt tattgtttac
      661 taattactgt ttacttactt tcttttattt attgggctga tctctatttt aaccacatga
      721 ccaaaactca cccctccgac tggctagttg gctacaaaga agccatggtc tatataatcc
      781 aaaaacagga tcaatatcaa acagttacct tcaccgactt ttacggccag ccctacattt
      841 actatctttt ttattcgaaa tatccaccag ccaaatatca acaacaagcc caattaattt
      901 cttcttcacc agacgtcggc caaattaaaa aaatagacaa gattaacttc ggtccggcca
      961 acttcccccc agtcaataat tcattgactg ttttttctta tgacgaaatt gtccgacaaa
     1021 atcttgattt caaaaacttc actagatttg gcaacttcta cgcctatgaa aacccataaa
     1081 atatttttta tcttaattct cgctatcgtt ctccgttttc tctttatcgg cactatgccg
     1141 gcgcttaatc ccgatgaagc cgctcttggt tataatgctt actctctctt aaaaactggt
     1201 aaagacgaac atggttcatc ctggccaatt cactttaaat ctttcggcga ttacaaacct
     1261 gggggctacg tttacttagc cctgcctttc attaaaattc taggtttaac cccgttggct
     1321 actcgtttac ccaaccttat tctctcaatt ttaaccattt attattttta tcttctcatc
     1381 aatcaactat caaagaatga caatttagcc gccctttcgg ctttgattct aacgatatct
     1441 ccttggcatt tacatttttc tcgaggagct tgggaagctg gagctgccct ggcttttacg
     1501 gttattggca cttattgctt tttcaaaaat caaaaatacc tttttattct tttcttctct
     1561 ctatctcttt atacctatca ttccgcccgc cttatcacac ctcttctagc aatcattctt
     1621 ataatattaa actataagat agaaattaga aattggaaaa aatggctttt acccattgtc
     1681 ttcgggatta tcatttgtct gccggttctg ttctccttcc ttcatcaagg cggagccagc
     1741 cgtctaaacg gcgttggttt agccgccgat aaaggaccat taaaccgagc cgaggaatta
     1801 ttaaatcaac atggcaacac taaaattttt aaccgtatta tccataataa acgtgtttta
     1861 tatcttttat cttgggggca aaaatacgct tctcatttca atcctaattt tttatttatc
     1921 aacggcgatg aagttcctcg atcaaaagta ccggaaatgg gtctcctgta tcttatcgaa
     1981 ctgccccttc ttatttacgg tttttatgaa tctaaaaaaa gaaaccaatc gaatctttta
     2041 atgttttctt ggcttttaat cgccccgatt gcttccagtt taacttttca ggcgccgagt
     2101 gctctccgtt cccttcctat ggttgttcca ttatcttact ttattgccgt gggtataatt
     2161 aacacccaaa aagtttatcc gccggaaggc gggcgggcca tttactgtct actaattgcc
     2221 atttacttgt tttcttttgc ttattacctc gacgcctact accgccatta ccaaaaacgt
     2281 tggccctttg cctggaatta tggctttgct caaattgttc cttacgttga atcgcaaaaa
     2341 aaccagtacg atcatatttt ctttactaat aaatttgacc agccatacat tctctattta
     2401 tttttcagcc aatatccgcc ggcccaactt caaccccaaa ttaaattaac tccgcctgat
     2461 caatatggct tttccactgt cgatcaaatc gataacatca cttttcatat ccctcccgaa
     2521 aaagatattc cgccccattc tttagtcatt tctgataaaa tgctcttacc atgaatcttt
     2581 ccgtcactgt tgctacttat aatgaggagg ccaatatcgg tcatttttta aacaacgtta
     2641 gtgatatcgc tgatgaaata attattgttg acggtaaatc aaccgacaaa acagttaaaa
     2701 tcgcccggga attcgccagg cgacaaacaa caactaaaat caagattatt atcactgata
     2761 ataaacccat gtttcatttt aataaacaaa tgggtattga tgcctgtcag ggcaattgga
     2821 ttcttcaatt agatgccgat gaacgattaa ctcacgaatt aaaaaaagaa attttggaaa
     2881 tagcagggaa ggatcctcaa acagtggaat tcaacggcta ttggcttaaa cgcaaaaatt
     2941 atttcctcgg tagtttttta aaaaagggcg gtcaatatcc tgatccaacc cttagatttt
     3001 ataaaagggg taggggacat ctgccttgcc gcgatgttca tgaacaagca gttgttaacg
     3061 gcaaagttgg ctggttaaaa catgatttgg aacattatgc cgacccttct ttttctcgct
     3121 atctcctccg ggccaatcgc tacacgaccc tcttagctca gcaattagtc ggccaaaaag
     3181 taccaatcaa ttttctctct tttgtaaaat attattttgt tctgccccaa ggatggtttt
     3241 taaaaacttt tttccgccac cgcggctacg tcgacggttt taggggattc gttttttcct
     3301 ttttttccgc tctccgtttc cccatcgcct acactaaata cttcgaatta aaacgcaata
     3361 tttcccaagg gtgggaataa caatgatctc caaaataaaa atcggtttcg ttagttcacc
     3421 tttaaataat caaaataaag ttcgcggcgt cggctattac accaaaaaac tttacgaaaa
     3481 tttatccaaa ataaaaacct tagaagttaa tttaattaaa aattggaatt taaaaattga
     3541 aaattatgac cttatccatt atcctttttt tgatcctttt aatttaaccc ttaaaatttt
     3601 taaacagcca actgtagtta cggttcatga tcttattcct cttcaattta aaaaacattt
     3661 ccccgccggt tttaagggca atttaaaatg gctgattcaa ctttatcatc ttcgtcgagc
     3721 agcggccatc atcactgttt ctcaatattc aaaaaaaatt attcatcaaa tcactcacat
     3781 acctctcaat aaaatcttcg tcacttatct agcggctgat tctactttta aaccgattaa
     3841 taacaagtct tatcttcagg ctattaaagc caaatatcat ttgcctgata aatttgtctt
     3901 atttgttggt gatattaatt ggaataaaaa tattcctagt ttagtaaaaa tttgtcaaca
     3961 tttaaattat cctttggtca ttgctggttc agctgccgtc caaaaaaacg ttccgattca
     4021 tccttggact aaagatttac tttggctcca acagcaaaaa taccctctta aattaggctt
     4081 cgttcccgaa accgaattac cagctttatt taatttggcc accatttatt gccaaccatc
     4141 ttatgctgag ggctttggtt tgccgccgct acaagcgatg gcttgtggtt gtccggtaat
     4201 ctgccgaatg gctaccagtt tacccgaagt agttggttcg gctgctttgc ctttttctat
     4261 cgatagtttt caaaaatttt ggcaggaaaa aaaaattaga gaaaaatata ttaaacttgg
     4321 actagaccag gctaaaaatt tttcttggga aaaaacagcc cgggcgacta tggaagtcta
     4381 tgtcaaaatt aactcctaat cattttctat ccttaattgt cccggtttat aaacaggaaa
     4441 agaccataaa aaaagatatt gtccgactac ttaaaactct cgatcaaatc cgctatccct
     4501 ccgaactaat cgccgtcgtt gacggtattc tggtcgacac ttcttattac attcttaaaa
     4561 aaataaaaaa ccctaaactt aaggtaatcg cgattaaacg caatcaaggc aagggttacg
     4621 ccatccgcaa ggggatggct aaggcaaaag gggattatat cgcctttatt gatgccggca
     4681 tggaaattga tcctaatggc atttccatga ttttagaaca tatggaatgg tataacgccg
     4741 atattattgt tggctcaaaa cgtcatcccg cttcaattat acaatatcca ttagatagga
     4801 aaattattag cttctgcgcc cagatttatg ctcgtctttt tctgggccta aaaattaccg
     4861 acactcaggc tggtttaaaa attttcaaac gcagagtttt ggaaaaaatc ttgccaaaat
     4921 tactagtaaa acaatacgct tttgatctgg aaattctagc agttgctaat cgtcttggtt
     4981 ttaaacgaat ttatgaagct ccaatcaaac ttaactatag ctttactagt ttaacccacg
     5041 ctaatagttt aaacaccatt tacaaaaccc taatcgactc cttggctgtt ttctaccgct
     5101 taaaaatcct tcattactat gacaaaaaaa attgattttt cggtcattat tccagtccga
     5161 gctcccaata gctacctgaa ggaaacttta gctcatttaa aacaacaaat ttataaacaa
     5221 tttgaagtct tagttattac cgacaatgtt tctaaaactc ctaatccagc cgccaaaaga
     5281 aatctcggcg ctaaaatggc caagggaaaa tatcttgctt tccttgatga tgattcatat
     5341 cctgatcgtt tctggttaaa aaatgccaaa aaacaattag aaaaaaaatc gattattgct
     5401 gccgttggtg gtccttgtct aacccc
//
LOCUS       DAOOIE010000021         5287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_12484_length_5287_cov_4.370604, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000021 DAOOIE010000000
VERSION     DAOOIE010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12484_length_5287_cov_4.370604"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2410
                     /locus_tag="PK131_01095"
     CDS             <1..2410
                     /locus_tag="PK131_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44761.1"
                     /translation="LADQACFLTVNDYYAYFKSADSVTGSHKNAAQLKKYIKDIQTCF
                     SFIHQFLKEIGLAKIYQNDFNGWLSLYARMWRNSIQHSRLSPTDKTSLISYLKVKLKQ
                     KKILNFNDNDNYFYKLQTEINPQYELLKRKVASSKYRYISFDLFDTLVVRPFWEPHDL
                     FKLLDRDFQKLNQKTIINFHDLRLRAESAVRQEKWRNGSKEEITLDEIYLYIEKNYQI
                     SKTVLDVIKRKEINLELEFCTARKSVLEIYNLAVYLNKKIICISDTYLPRRVIKKILT
                     KNGYTRFERVFLSSEVKLTKHTGHLFGYVVNQLKCQPSAILHIGNDYFSDIKSAERNN
                     FAGFFYPSPTAVFKNEVSSLSTSDCGALFIQNKGLWVRTIHALFFLGIRCMLALAANK
                     YFDNPFRPFNRNSDFNADPYFVGYYPLGMHLLAVDKWLMENVIETRKTETIQFMARDG
                     YLAMKGWEILNKVYHSKIKTNYLYINRRVLIPLFFTDKIDFFYLFNITKLESILNYSR
                     PIIGNYSELMTDLKRNKINYRKNINNPTKVLKVIMKHIDSGKQRQYSDLLKRYFTDNI
                     GKYDAIFDIGYSSRPLFFIKKLTGLTTESYFIHVNQEEAAELAAKASFKIKTFYDFTP
                     AITGVAREIIMSESHPACIDFCLDKNDLVQPVFEDQLPSYQEKIAAGLLEKGVLDFIG
                     DYTGIFKKYFFDLHFRYLDASLPVEMFLEGSKKFDRDILSCFTFDDSVGGLGLINAVG
                     FWENELASRNINNFNTPIELGQIKRSTGYRFLLKWYKIRDLFIPENSRRRKLLKKIFS
                     QLSK"
     gene            complement(2391..>2605)
                     /locus_tag="PK131_01100"
     CDS             complement(2391..>2605)
                     /locus_tag="PK131_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44762.1"
                     /translation="PEVIIDLSLKKQGMKIVKERQKITVPELAAELHLLQDDLRILAK
                     GWMAGGGGSKMNFDLTPPGEFTWIAD"
     assembly_gap    2606..2705
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2939..3808
                     /locus_tag="PK131_01105"
     CDS             2939..3808
                     /locus_tag="PK131_01105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPD44763.1"
                     /translation="MEKKWYCLAIDEIQSILKANFQRGLTEAEAKIRLKKFGFNQLPE
                     EKPLSKVKIFFEQFQSPLVYILIIAGIVTLLLKENVDAVVIFTAVLLDAVVGFLQENK
                     AGQILKTLKKAVKNEAEVLRDGNLKIIDASQLVPGDIIILTAGSRIPADGRIFESHDL
                     KVNEMALTGEWLPAEKRTGVLDENVPLADRDNLVYSGTMIEDGKGRAVVTATGLKTEI
                     GKIAAMLKETREEKTPYQKKIANFSRTIGIIISIVCLIIFIEGILTEGNWIEMFVTAV
                     AIAVAAIPEGAYQ"
     gene            3811..>5287
                     /locus_tag="PK131_01110"
     CDS             3811..>5287
                     /locus_tag="PK131_01110"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPD44764.1"
                     /translation="MTVILAVGMQRILKKGGLVRRLASAETLGSTSIIATDKTATLTE
                     GKMKVVEVIGDKSLILKTVVLTSQAFIENPEASVDQWIVRGESTDQALLLAAITAGFN
                     KDELDKHKIAEMPFNPANKLAATVLKEAGRNNLYVCGAPEAVLALSQLTAREGQQWKK
                     KLDQLAGKGLRIVAAGYKTDLNSAIDFKEIINNLTFTGLIALRDPVRPEVKEAINLCR
                     QAGMRPIIITGDHKLTAKAVTEELDWNIGDEDILEGKDLDKLNDDGLDKILTRIQIYA
                     RVEPKDKMRIITAWQKRGKVVAMIGDGVNDAPALKKADIGVALGSGTEVAKEAADLIL
                     LNDSFAIIVAAVEEGRAILDNIRKVITYLLSDTFSEVVLVGVSILAGVPLPITAVQIL
                     WINLMEDGLGDIALAFEPKEEDLMKQKPQGHDLPLLTKEMKTIIFIIGLITDLILLGL
                     FFWLWRQNYPIDYIRSIIFTTLTIDSLLYIFSCKSLRKNIWQ"
BASE COUNT         1655 a          813 c         1057 g         1662 t
ORIGIN      
        1 cttggctgat caggcctgct ttttaacggt taatgattat tatgcctatt ttaagtcggc
       61 tgactcagtt actggtagtc ataaaaatgc cgcccaacta aagaaatata ttaaggatat
      121 tcagacgtgt ttttctttta tccatcaatt tttgaaagaa ataggtttag caaaaattta
      181 ccaaaatgat tttaacggct ggttatcact ttatgcccgg atgtggcgca attccattca
      241 acattccaga ttgtctccaa ctgacaagac gtcgttaatt tcatatttaa aagtaaaatt
      301 aaagcagaaa aaaatactta attttaatga taatgataat tatttttata aactgcagac
      361 agaaattaac cctcaatacg aattattaaa acgaaaagtt gcctcttcca aatacaggta
      421 tattagtttt gacttatttg atacgttagt tgtcaggccc ttttgggagc ctcatgattt
      481 gtttaaattg ctcgataggg attttcaaaa attaaatcaa aaaacgatta tcaattttca
      541 tgatcttcgg cttcgggctg aatcagcggt caggcaggaa aaatggcgga acggttcgaa
      601 ggaagagata actttggatg aaatctattt atatattgaa aagaactatc aaatatccaa
      661 aaccgttttg gacgtaatca agcgaaaaga aataaatttg gaattggagt tttgtaccgc
      721 ccggaaaagt gttttggaaa tttataattt agcagtttat cttaataaga agataatttg
      781 catttcagat acttatttgc cgcggagagt aattaaaaaa atattaacta aaaacggcta
      841 tacccgcttt gagcgggtat ttttatcatc cgaagtaaaa ttaaccaaac ataccggtca
      901 tttatttgga tatgttgtta accaattaaa atgccagcca tcggcaatct tacatatagg
      961 taatgattat tttagtgaca ttaaatcagc tgagagaaat aattttgccg gtttctttta
     1021 tcccagcccg actgctgttt ttaaaaatga agtcagcagt ttgtctacca gtgactgcgg
     1081 tgctttattt attcaaaaca aaggtctatg ggtgagaaca attcatgcct tgtttttctt
     1141 ggggataagg tgcatgctgg ccttggcggc taacaaatat ttcgataatc ccttcaggcc
     1201 gttcaatcga aattctgatt tcaatgccga cccatatttt gtgggttatt atcccctggg
     1261 aatgcatctg ctggctgttg ataaatggct gatggaaaac gttattgaaa caagaaaaac
     1321 agagacaatt caatttatgg ccagggatgg ctatttagcc atgaaaggct gggaaatttt
     1381 aaataaagta taccattcca aaattaaaac taattattta tacattaatc ggagagtttt
     1441 gatacccctc ttttttaccg ataaaatcga tttcttttat ttatttaaca tcactaaatt
     1501 agagtcaatt ttaaattaca gtcggccaat tataggaaat tattctgaac ttatgactga
     1561 tttgaagcga aataagatta attatagaaa aaatattaat aatccaacca aggttttaaa
     1621 agtaattatg aaacatatcg attctggtaa acagagacaa tattctgatt tgttaaagcg
     1681 atattttacc gataatatcg gaaaatatga tgccattttt gatattggct atagtagccg
     1741 gccgctcttt tttattaaaa aattaactgg actgacaact gaaagttatt ttattcatgt
     1801 taaccaagaa gaagcagccg aattagccgc taaagcgtca tttaaaatta aaacttttta
     1861 tgattttact ccggcaatta ccggtgtcgc tcgggaaata attatgtctg aatctcatcc
     1921 tgcctgtatt gatttttgtt tagataaaaa tgatttagtt cagcctgttt ttgaagatca
     1981 gttaccttct tatcaggaga aaattgccgc tggtttattg gaaaaagggg ttttagattt
     2041 tatcggggat tataccggta tttttaaaaa gtattttttt gatcttcatt ttcgctatct
     2101 tgatgcctct ttgccagtag agatgttttt agaaggctcc aaaaaatttg accgtgacat
     2161 tctttcatgt tttacctttg atgattctgt tggaggattg ggattaatta acgctgttgg
     2221 attttgggag aatgaattag cttcgagaaa tataaataac tttaatactc ccatagaatt
     2281 aggacaaatt aaaagatcaa ccggttatcg ttttttattg aagtggtata aaattaggga
     2341 cctttttatt cctgaaaaca gtcgccggag aaaattgtta aaaaaaatat ttagtcagct
     2401 atccaagtaa actcccccgg aggagtaagg tcgaaattca tttttgaacc gccgccgcca
     2461 gccatccaac ctttagctaa tattctaaga tcgtcctgaa ggaggtgaag ttcagctgct
     2521 aattcaggaa cggttatttt ttgcctttcc ttaactattt tcatcccctg ttttttaaga
     2581 cttaaatcaa taattacttc cggccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnntccgg ccgctttcca actattagca gccagttcaa atccttcttg attgagagcg
     2761 aattggggaa tagttgtaat taaggtggtg gttataatcc cgatcaaaat caaaatactt
     2821 atcatcggcc cggccaatct ggttactttc agaaaccaat gaataactct gcctgtaaaa
     2881 gatatttttg ttttgataat tttattttat acccttaatg ctaaactaga aaagggatat
     2941 ggaaaaaaaa tggtattgtt tggcgataga tgaaatacaa tccattttaa aggctaattt
     3001 tcagcggggg ctaacggaag cagaagctaa aatcagatta aaaaagtttg gtttcaatca
     3061 attgcctgaa gaaaagcctt tgtccaaggt aaaaatattt tttgaacaat ttcaaagtcc
     3121 tctggtttat attttaatta ttgccggtat tgttactttg ctgttaaagg aaaacgtcga
     3181 tgccgtagtt atttttacag ccgttttatt agatgctgtt gttggctttt tacaggaaaa
     3241 taaggctggc cagattttaa agactctgaa aaaggcagtt aaaaatgagg ccgaggtttt
     3301 aagggatgga aatttaaaga ttattgatgc cagccaattg gttccgggcg atattataat
     3361 tttaactgcc ggcagccgga ttccggctga tggccgaatt tttgaaagcc atgatttaaa
     3421 agttaatgaa atggctctta ccggtgaatg gcttccagct gaaaaaagaa ccggagtttt
     3481 ggacgaaaat gtgcctttgg ccgatagaga caatttagtt tattcgggaa caatgattga
     3541 agatggcaag ggtagggctg tagttacagc taccggccta aaaactgaaa ttggaaaaat
     3601 tgccgccatg ttgaaagaaa ctcgggaaga aaaaacgccc taccagaaaa agatagctaa
     3661 tttttcaaga accataggaa taattatcag catagtttgt ttgattattt ttattgaagg
     3721 aattttaact gaaggtaatt ggatagagat gtttgtcacc gccgtggcta tagcggtagc
     3781 ggctattcct gaaggggctt accagtagcg atgacagtta ttttagctgt aggtatgcag
     3841 agaatcttaa agaagggcgg gttagttcgc cgattggcct cagccgagac cttgggcagt
     3901 acttcaatca tcgccaccga taaaacagcg accttgactg agggaaaaat gaaagtcgtc
     3961 gaggttattg gtgataaatc tttaatctta aaaacagtgg ttttaacttc ccaggctttt
     4021 atagaaaatc ctgaagcttc ggttgatcag tggattgttc gaggtgagtc aactgatcag
     4081 gctttattgt tggcggctat cacagctggt tttaataaag atgaattgga taagcataaa
     4141 attgccgaaa tgccatttaa tccggctaat aaattagcgg cgacagtgtt aaaagaagcc
     4201 ggccggaata atttatatgt ttgtggtgct ccagaggcag ttttggcctt atcccagtta
     4261 actgcccggg aagggcaaca atggaagaaa aaattggatc aattagccgg aaagggtcta
     4321 agaattgtag cggcagggta taaaacagac ctaaattcgg ccattgattt taaagagata
     4381 attaataatt taacttttac cggtctaatt gcccttcggg atccagttag gccagaggtt
     4441 aaggaggcga ttaatctttg ccgccaagct ggtatgagac caattattat taccggggat
     4501 cataaattaa ctgccaaagc ggtcactgag gaattagatt ggaacatcgg agatgaagat
     4561 attttagaag ggaaagatct ggataaatta aatgatgatg ggcttgataa gattttaacc
     4621 agaattcaaa tttatgcccg ggtggagcct aaagataaaa tgagaattat taccgcctgg
     4681 caaaaaagag gaaaagtggt ggctatgatt ggtgacggag ttaatgatgc cccggctttg
     4741 aaaaaagccg atattggggt agctcttggt tcgggaacag aagtagctaa ggaagcagcc
     4801 gatttaattt tattaaatga tagttttgcg attattgttg ccgcggtcga ggaaggtcgg
     4861 gctattttag ataatattag aaaagtaatt acttatcttt tatccgatac tttctctgaa
     4921 gtagttttgg ttggggttag tattttagct ggtgtgccct taccaataac tgccgtccaa
     4981 attttatgga ttaatttaat ggaagatgga ttaggagata ttgctctggc gtttgaacct
     5041 aaggaagagg atttaatgaa acaaaaaccc caaggacatg atctgccttt attgactaag
     5101 gaaatgaaga caattatttt tattatcggt ttaattactg atctgattct tttaggatta
     5161 tttttctggc tgtggcgtca aaattaccca atcgattata taagatcaat tatttttacc
     5221 actttaacaa ttgattcttt gttatatatt ttttcctgca aaagcctacg gaaaaatatc
     5281 tggcaag
//
LOCUS       DAOOIE010000022         5154 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_12855_length_5154_cov_3.474015, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000022 DAOOIE010000000
VERSION     DAOOIE010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5154)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5154)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5154
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12855_length_5154_cov_3.474015"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="PK131_01115"
     CDS             <1..387
                     /locus_tag="PK131_01115"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HPD44765.1"
                     /translation="KIAKSNRKNPTPAEKIFWQSIIGRDKLKYRFLRQKPVGRCILDF
                     YCSSLLLDVEIDGNSHDSKKGWDKARDEYLTMRGIKTIRYQNNEVLYSTDKVIKDLAT
                     KIKERENELLLTPSFSKRGQGEISNE"
     gene            complement(469..1335)
                     /locus_tag="PK131_01120"
     CDS             complement(469..1335)
                     /locus_tag="PK131_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPD44766.1"
                     /translation="MIHKAVITAAGRGTRFLPAVKSYPKELIPIMDKPNIQLLVEELI
                     AAGIKEICIVHRLGDTAIKNYFTPDLELEKFLKNNNKLAFLDSLRAIWEAAKLEFLPQ
                     TDDLPYGNASPALAAKDFIGQDDFVYVFGDDLIIEEKPGEYLKKIIAAFETNHLDIAV
                     CTQEMPTEEMKRYGAVVPAENPAIPNQVSGLLEKTDPAPSNFAVVGRYITKNKIMDVI
                     QHQSISRDNELWWADAANTVIQNGLGAHEPITHGVWMTTGDPLRWLKATITYALKQPQ
                     YQEDLKGFLKRV"
     gene            1401..1473
                     /locus_tag="PK131_01125"
     tRNA            1401..1473
                     /locus_tag="PK131_01125"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1434..1436,aa:Ala,seq:ggc)
     gene            1523..2086
                     /locus_tag="PK131_01130"
     CDS             1523..2086
                     /locus_tag="PK131_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD44767.1"
                     /translation="MVNLKNKVVCITGLTGAGKSVVADFFVKNNFQYIRLGQITIDEI
                     EKRNLPVNEDNERKIREEFRAKYGMAAYAILNLTKIESLIKKGHVIVDGLYSFEEYKV
                     LKDKFGDALVVVTVYAPPRIRYERLSRRQERPLTIKEAQSRDFAEIENLNKGGTIAMA
                     DYTILNISDWKFLQKQTIKVKNDIIGK"
     gene            2091..2161
                     /locus_tag="PK131_01135"
     tRNA            2091..2161
                     /locus_tag="PK131_01135"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2123..2125,aa:Trp,seq:cca)
     gene            2259..2537
                     /locus_tag="PK131_01140"
     CDS             2259..2537
                     /locus_tag="PK131_01140"
                     /inference="COORDINATES: protein motif:HMM:NF016432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HPD44768.1"
                     /translation="MGKNKECLSLNPFLVRNVAVDDGSETVILKLSIEEGLRRVTPRE
                     AEAIKLLYGIDDGIERTPKEVGMIMGVTRQRVSSLEKQALKKIKDFLS"
     gene            complement(2538..2717)
                     /gene="rpmG"
                     /locus_tag="PK131_01145"
     CDS             complement(2538..2717)
                     /gene="rpmG"
                     /locus_tag="PK131_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPD44769.1"
                     /translation="MAKTKKTSRIILALNCSICGAQNYITEKNKTNTPDKLKLIKYCR
                     WCKKRTEHKESTKLK"
     gene            complement(2792..3586)
                     /locus_tag="PK131_01150"
     CDS             complement(2792..3586)
                     /locus_tag="PK131_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPD44770.1"
                     /translation="MKPLLIIPLFYQLLTAYQSQATATLTVIPIPTPTSTPRAEIIVK
                     ENPAFAAQNIGGVSFLTDKRTIAVIGDSMIETLQPNLPDLNTALNKYYPDTAFNLINL
                     GYPAQNLEYVQKRLQEDLFPHKPDLVVIESFAYNNFGNSSNGINRHWLDLGALTTTIK
                     KELPNTRIVLAATLAPNSVIFGNNFSSMEKIEKTKTIKLYLQNLVNFANSENFPLANA
                     YQYSLDNNNEGLPDLIDSTDHIHPSPAGAQLFSDIIAKTIFDNKLL"
     gene            complement(3583..3867)
                     /locus_tag="PK131_01155"
     CDS             complement(3583..3867)
                     /locus_tag="PK131_01155"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HPD44771.1"
                     /translation="MINIQIYSENFPLSESMRALINERLAVPLDKYVTHWPEDQKNAQ
                     LHITKEGPELFKVNFSMNNLYSEARHVNFEAAIVDLREQLEIQIKKLKKA"
     gene            3933..>5154
                     /gene="purB"
                     /locus_tag="PK131_01160"
     CDS             3933..>5154
                     /gene="purB"
                     /locus_tag="PK131_01160"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPD44772.1"
                     /translation="MKRKEKFISRLDFSEYLSVFSWRYGSKDMRVIFGEKHKYELWRK
                     IWVALAKAQFKAKLVSKRELDDLVRNQNKINISRIFEIEKETKHDVVAAIREYAEEAK
                     IGGGKIHLGATSMDITDNTDVLKIKEALIIIEERLKKVLKEFKEKIECYADVPCLGLT
                     HLQPAEPTTVGYRLAFYAQDLLNDLKFLEFVQKNLRGKGMKGAVGTGASYQRGIEKAV
                     MESLGLKAFLISSQVYPRKIDYWLLAMLAGIGSSLAKFSADLRILQSPMLGEWSEPLT
                     EKQVGSSAMPFKRNPINAEKICSLARYLLFLTPVALENNSLSYLERTLDDSANRRIII
                     PEAFLTAEEILITAEKLINGLVINEDKIRDNLRQYAPFAAMEMTLIDAVKQGADRQKA
                     HEAIRKRAMKAWEAV"
BASE COUNT         1603 a          791 c         1246 g         1514 t
ORIGIN      
        1 aaaattgcaa aatcaaatag gaaaaatcca accccggcag agaagatatt ttggcaatca
       61 attatcggta gagacaagct gaaatatcga ttcttgagac agaagccggt aggcagatgt
      121 attcttgatt tttactgctc cagtttgtta ttagacgttg aaattgatgg taactcccac
      181 gattccaaaa aaggttggga taaagctaga gatgagtatt tgacaatgag gggaatcaaa
      241 actattagat atcagaataa tgaagttctt tattccaccg ataaagtcat aaaagattta
      301 gcaactaaga taaaggaaag agaaaatgaa ctccttttaa ccccctcttt ttcgaagagg
      361 gggcaggggg agatttctaa tgaataattc agagatcgag gagggggcag ggggagattt
      421 agattaatta gaaactagtc attgtttgtt tctcggaatt tggagggttt aaactctttt
      481 cagaaaccct ttcaaatctt cttgatattg gggttgttta agagcgtagg taatagtcgc
      541 tttcagccag cggagaggat cgccggtggt catccagacg ccatgggtaa ttggttcatg
      601 agcaccaagg ccattttgaa taacagtgtt ggcggcatca gcccaccaca gctcattgtc
      661 acgggagatg ctctgatgtt gaataacatc cataatttta tttttggtaa tataacggcc
      721 gaccacggca aaattagacg gcgccggatc ggttttttcc aaaagaccgg aaacttgatt
      781 gggaatggcc gggttttccg ccggaacgac agccccatag cgtttcattt cttcggtcgg
      841 catttcctgg gtgcaaacgg cgatatctaa atgattggtt tcaaaagcgg caatgatttt
      901 ttttaaatat tcgcctggtt tttcctcgat aattaagtcg tcaccaaaaa cgtaaacgaa
      961 atcatcttgg ccgataaaat ccttggcggc cagagccggt gaagcattgc catagggaag
     1021 gtcgtcggtt tggggcagga attcgagttt agccgcttcc caaatcgccc gaagtgaatc
     1081 taaaaacgct aatttattat tattttttaa gaatttttct agttctaaat cgggagtgaa
     1141 gtaattttta atggcggtat cgcccaaacg gtggacaatg cagatttctt tgatgccggc
     1201 agcgataagc tcctcgacca gaagctggat gttgggtttg tccatgatgg gaataagctc
     1261 tttgggataa gatttgacgg cgggtagaaa acgagtgccc cgtccggcgg cggtaataac
     1321 agctttatga atcataaagt tatgataaca tattaggttt tgggaacgga gaaaatttga
     1381 tagaatattt aagttttaaa gggcctatgg cgtagttggt agcgcgtccc catggcatgg
     1441 ggaaggtcag gggttcaagt ccccttaggt ccacagtcag cctttggctg aagtttttat
     1501 ttttaataaa aagaaaagcg gaatggttaa tttaaaaaat aaagttgttt gcatcactgg
     1561 attgaccggg gcagggaaga gtgtggtggc agattttttt gtaaagaata attttcaata
     1621 tatcaggctg ggacagatta ctattgatga aattgagaaa agaaatttgc cggttaatga
     1681 agataacgaa agaaaaatta gggaagaatt ccgggcaaaa tatggcatgg cggcctacgc
     1741 cattttaaat ttgaccaaaa ttgaatcctt aataaaaaaa ggtcacgtaa ttgttgatgg
     1801 tttatattct ttcgaagaat acaaagtttt gaaagataaa ttcggtgatg ctttagtagt
     1861 ggtaactgtt tatgctccgc caagaataag gtatgaaagg ttaagtcgga ggcaggagcg
     1921 gccgttgaca attaaagagg ctcaatcacg tgatttcgcc gaaattgaga atttaaataa
     1981 aggaggaaca attgccatgg ccgattatac aattttaaac ataagtgatt ggaaattttt
     2041 acagaaacaa acgataaaag tcaagaatga tataattgga aagtaaaatt aggcctgtag
     2101 tgaagtggta tcaccgcagt ctccaaaact gctatcctag gttcgaatcc tagcaggcct
     2161 gctggacaga gcctattttt atttactgga gtctatagct aaaataggag cgatattttg
     2221 ggggttgact aattcaattt gatctagtat agtccgctat gggaaaaaat aaagaatgtc
     2281 ttagtcttaa tccgtttttg gtgaggaatg tggcagttga cgacggttcc gagacggtta
     2341 tcttgaaatt atctatagaa gaaggattac gtagggttac cccgagagaa gcagaagcca
     2401 ttaaattgtt atacgggatt gatgatggca ttgagagaac tcctaaagaa gtaggaatga
     2461 taatgggagt aacgcgccag agggtaagca gcttagaaaa acaagccctg aaaaaaatca
     2521 aggattttct ttcatagtta cttgagttta gtagattctt tatgttcggt tcgtttttta
     2581 caccagcggc agtattttat cagctttaat ttatcagggg tgttggtttt gtttttttcg
     2641 gttatataat tttgagctcc acaaatagag cagttaaggg cgagaataat ccgcgaggtt
     2701 ttcttggttt tagccatgaa aagaattata acagataata aatgagaaat ctcctcctcc
     2761 cgctctccgc cggctggcgg gaaaaaggtt tttacaatag tttattgtca aaaatagttt
     2821 tggcaataat atcactgaat aattgagctc cggcagggga gggatggata tgatcggtag
     2881 aatcgattaa atcgggcaaa ccttcgttgt tgttatcaag gctgtattga taggcgttag
     2941 ctaggggaaa attttccgag ttagcgaaat tgactaaatt ctgcagatag agtttaatag
     3001 ttttggtttt ttctattttt tccatactgg aaaaattatt accaaaaata actgaatttg
     3061 gggccagagt agcggctaaa acaattcggg tattaggcag ttcttttttg atagtggtgg
     3121 ttaaagcgcc taagtccagc caatgacggt tgataccgtt gctactgtta ccaaaattat
     3181 tgtaagcaaa agattcaatg acaaccagat cgggtttatg gggaaataaa tcttcctgga
     3241 gtcgtttttg gacatattct aaattttggg ctggataacc taaattaatc aaattaaacg
     3301 cagtatctgg ataatatttg ttaagggctg tgtttaaatc tggtaaattg ggctgaagag
     3361 tttcgatcat tgaatcgcca atgacggcaa tagttcgttt gtcggttaag aaagaaacac
     3421 caccaatatt ttgtgctgca aaagcaggat tttcttttac aataatttcg gctcttgggg
     3481 tagaagttgg agttggaatc ggaatgacag tcagagtagc agttgcctga gactgataag
     3541 cagtcagaag ttgataaaat aggggaataa taagcagggg tttcatgcct ttttcaattt
     3601 tttaatttgg atttctaatt gttcccttag gtcgacaatt gcggcttcaa agttaacgtg
     3661 gcgggcttca gaatagagat tattcatgga aaaattaacc ttaaaaagtt ctgggccttc
     3721 cttggtgata tgcaattgag catttttttg atcttcaggc caatgagtta catacttatc
     3781 taaagggact gctagccttt cattgatcaa agcgcgcatg ctttcggaaa gaggaaaatt
     3841 ttcggagtag atttgaatgt taatcatctg tttttagatt aacataaatt tgaggatttt
     3901 tttttgacaa cttttggggg ataatagagc tgttgaaaag gaaagaaaaa tttataagcc
     3961 ggttggattt ttctgagtat ttatcagttt tttcttggcg atacggcagt aaagacatga
     4021 gggttatttt cggtgaaaaa cataaatatg aattatggcg gaaaatttgg gtggctttgg
     4081 ctaaagccca atttaaagct aaattagtat caaaaagaga attggatgat ttagtcagga
     4141 atcaaaataa gattaatatc agtcggattt ttgaaattga gaaagaaaca aaacacgatg
     4201 tcgtagcggc aattagagaa tatgccgagg aggcaaaaat cggcggcggc aagattcatc
     4261 tcggggccac cagtatggat attaccgata ataccgatgt tttaaagata aaagaagctt
     4321 tgataattat tgaagagaga ttaaaaaaag ttctaaaaga atttaaggag aaaattgagt
     4381 gttatgctga tgttccctgc ctcggtttga ctcatttaca gccggcggag ccgacaactg
     4441 tgggttaccg cctggccttt tatgcccagg atttattgaa tgatttgaaa tttttggaat
     4501 ttgttcaaaa gaatttaaga ggcaagggaa tgaagggggc ggtggggacg ggagcaagct
     4561 atcagagggg gattgagaaa gcagtgatgg aaagtttggg actaaaagcg tttttaatca
     4621 gcagtcaggt ctaccccaga aagattgact attggctgtt ggctatgctg gccggtatcg
     4681 ggtcgtcttt agccaagttt tcagctgatt taaggattct tcagtcgccg atgctgggtg
     4741 aatggtcaga gcctttaacg gaaaagcaag ttggttcttc agcaatgcca tttaaaagaa
     4801 atccaataaa cgcggagaaa atatgttctc tggcgcgtta cctccttttt ttaactccgg
     4861 tggcgttgga aaataattcc ctatcatatc ttgagagaac tttagatgat tcggctaata
     4921 ggcggattat tattccggag gcgtttttaa ccgccgagga aattttaata acggcagaga
     4981 aattgatcaa tggtttagta attaacgaag ataaaatccg agataatttg agacagtatg
     5041 caccatttgc ggcaatggaa atgacgttga tcgatgcagt taaacagggg gccgacaggc
     5101 aaaaggctca tgaggcaata agaaaaagag caatgaaagc ctgggaagcg gttc
//
LOCUS       DAOOIE010000023         5122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_12959_length_5122_cov_4.156898, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000023 DAOOIE010000000
VERSION     DAOOIE010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12959_length_5122_cov_4.156898"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="PK131_01165"
     CDS             <1..498
                     /locus_tag="PK131_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HPD44773.1"
                     /translation="KIKPVTRGKILKKFKENKINILVTTPIIEVGIDYPNATIIVIQS
                     ADRFGLASLHQLRGRVGRGEHQSYCYLFTESNEEKVFNRLKFLEKNNSGLKIAEYDLK
                     VRGPGEAFSTEQHGFPSLKLADISDLKLIEFSQNFLKDLLRLYPNFDPAKLIKTTHNK
                     INLSN"
     gene            682..1050
                     /gene="nusB"
                     /locus_tag="PK131_01170"
     CDS             682..1050
                     /gene="nusB"
                     /locus_tag="PK131_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPD44774.1"
                     /translation="MKSKRDPRHLHRIKEMQALFAYSCGNKKPVSLHTRDIIVHLAEI
                     DKIIAANAPKWPLDKINKIDLAILRLALWELLIQKKNPPKVIIDEAVEIAKEFGTDTS
                     FAFVNGVIGSALKNVPISQP"
     gene            1028..1690
                     /gene="rnc"
                     /locus_tag="PK131_01175"
     CDS             1028..1690
                     /gene="rnc"
                     /locus_tag="PK131_01175"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPD44775.1"
                     /translation="MFQFHNPNLLKQAFIHRSYLNENRQESLQSNERLEFLGDAVLEF
                     WVSDLLFKRFPQFPEGSLTNLRSLIVCTDNLAKTADKIGLGKKLLLSRGEEKHGGRTN
                     PSILADTFESFTGAIFLDQGWEAVDQFLTNNLGPSIDQISKQKNLKDPKSVFQEIAQA
                     REGVTPNYRILAEFGPDHQKKFEAGVYINNRLIAKGKGNSKQKAEEKAAIQATKIFQN
                     QV"
     gene            1699..1998
                     /gene="rpsP"
                     /locus_tag="PK131_01180"
     CDS             1699..1998
                     /gene="rpsP"
                     /locus_tag="PK131_01180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPD44776.1"
                     /translation="MLKIKLSPRGKKHQRTFRIVVAERRSKNKGQNIDEIGFFTPQTK
                     TINLDKAKMDQWLKHGAKITTGVDKLLNPAKYPHKKKVKQAKSLPKTNEPTPTNA"
     gene            1973..2206
                     /locus_tag="PK131_01185"
     CDS             1973..2206
                     /locus_tag="PK131_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPD44777.1"
                     /translation="MNQLQQTLEYILQSIIDNPEGVEVETKEQDNFTNLIISAPKEKI
                     GQIIGKQGKIIKAIRIILGISYPNTRFNLEIKE"
     gene            complement(2228..2983)
                     /locus_tag="PK131_01190"
     CDS             complement(2228..2983)
                     /locus_tag="PK131_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44778.1"
                     /translation="MTVTLPDKIFGLDSSLAKLLVKPIVIIILLIMLFNWGVYPKIAD
                     IGRLKQEINKVNAEKQMVVDKINYINSLPPEDLKKNESLLNSALLYEKDAYFLVGVIR
                     QIADKYSFTVQGFSLSPGKLTKDEKESGQAAVNKRVPVILNLIGPKTSYLNFLDGLEK
                     SLPILSIEKMNMRVRNEVAEMDLEISAYYLDEETKPEIKRLTLADLKLDKNEAEMLKT
                     LSNYTNNRTILSLAQEQIATRSGVVYGRSNPFR"
     gene            complement(2983..3519)
                     /locus_tag="PK131_01195"
     CDS             complement(2983..3519)
                     /locus_tag="PK131_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44779.1"
                     /translation="MSVSLLPSQAKFQLAKTKLKNQLLILMVTLTGIWLTASILIWGW
                     WLVRQRQLKVIQTAYNQVAAEYKNRGEDLLTSHRLKYQAKMVGKVLAARFEYGEAITN
                     VNNLLPSEITISDFKIQGHNAFQINYTTNKGENMDIVEEKVAEINNKKVKGFTAAKLT
                     SLSWESGQWKFSLEVKTE"
     gene            complement(3521..4555)
                     /gene="pilM"
                     /locus_tag="PK131_01200"
     CDS             complement(3521..4555)
                     /gene="pilM"
                     /locus_tag="PK131_01200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HPD44780.1"
                     /translation="MSNLVGLDLGVGMIKAVSLSKTANGLILDAIGETKGPNDDWVKK
                     GAKSDGIKQMAAAIRRLLADLKIRARQAVACIPEDEVVSRLVNLPPLKENEIMDALRF
                     EAETFVPYPLDQVTIDYEIVERDDNSRLKVFVVAAKTDLINAFIHLFRLVGIELIALE
                     SPAVAMRRVIKNSINPSVSGIITLDYGEKFFDIIGTNNGNICFTRSVPIGGESVTRAI
                     SINLGLDMPSAEEYKKAYGLREDQLEGKIRNAIMPIFMNMAEEIRKGLSLFLEDQGRP
                     AELLILSGGGANMPGLAEELTKVLGVEVQIVQPFLNIDTTKINFPFNLNTEGCRFSYA
                     VGLALRGIVE"
     gene            4606..4983
                     /locus_tag="PK131_01205"
     CDS             4606..4983
                     /locus_tag="PK131_01205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPD44781.1"
                     /translation="MAVIKDFYQLDTWKVNYKFVLEIYKCLDSLPKEEKYGIVDQLRR
                     ASSSITANIAEGFGRYHKKDKIHFYFQARGSLKEVQNFLLLSKDLGYINRQIARKLWI
                     SSKEAEKLINGLIRSTENNMKSS"
BASE COUNT         1651 a         1117 c          871 g         1483 t
ORIGIN      
        1 aaaattaagc cagtaacccg cggaaaaatc ctaaaaaaat tcaaggaaaa taaaattaac
       61 attttagtca ctacgccgat tattgaagtc gggattgatt atcccaacgc cacaattata
      121 gtaattcaat cagctgatag attcggtttg gcttccctcc atcagcttcg gggtcgggtc
      181 ggccggggag agcatcaatc atactgctat ctttttaccg aaagtaatga agaaaaagtc
      241 ttcaaccgac tgaaatttct ggaaaaaaat aattccggtt tgaaaatcgc cgaatacgac
      301 ctgaaagtcc gcggtccagg tgaagccttt tctactgagc agcatggttt cccttcgtta
      361 aaattggccg atatatcgga tttaaagttg atagaatttt cccaaaattt cctcaaagac
      421 ctcctccggc tttaccctaa ttttgatccc gccaaactta ttaaaaccac ccataacaaa
      481 atcaatttaa gtaactaaga ataaaaactg ttaaaataac ttcatatgac cgccttacct
      541 aaacattggc cttcaagccg ccgacaggga aaacgccggg caactcataa aggacccaaa
      601 attccccatc tagtcaaatg ccagcattgc ggccaagaaa aactacctgg ttttagatgt
      661 ccgaattgtc agcaataata aatgaagtct aaaagggatc cccgtcattt acatcgaatt
      721 aaagaaatgc aggcgctgtt tgcttactcc tgcggtaata aaaagccggt ttctcttcat
      781 actcgagata taattgtcca tttggcagag atcgataaaa ttatcgccgc aaacgctccc
      841 aaatggcctc tggacaaaat aaataagatt gacctggcga tactccggct ggcactttgg
      901 gagcttctca ttcaaaaaaa gaacccgcca aaagttatta ttgatgaggc agttgaaatt
      961 gccaaagaat ttggcaccga tacttctttt gccttcgtta atggagttat tggttccgct
     1021 ttgaaaaatg ttccaatttc acaaccctaa tttactcaaa caggctttta ttcaccggtc
     1081 ctatcttaat gaaaatcggc aggaatccct tcaatcaaac gaaagattgg aatttcttgg
     1141 cgatgccgtt ttggaattct gggtcagcga tcttttattc aaacgttttc ctcaatttcc
     1201 cgaaggcagt ttaaccaacc tccgttcttt aattgtttgt actgataatt tagccaaaac
     1261 tgccgataaa atcggtttag gcaagaaact gcttctttcc cgcggcgagg aaaaacacgg
     1321 cggccggact aatccatcaa ttttagctga tacttttgaa agttttacag gggcaatttt
     1381 cctcgaccaa ggctgggagg ccgtcgacca atttttaact aataatcttg gtccaagtat
     1441 tgatcagatt tccaaacaaa aaaatttgaa agatcctaaa agtgtttttc aggaaatagc
     1501 ccaagctcgt gagggagtta ctcccaacta ccgcattctg gccgaatttg gaccggacca
     1561 tcagaaaaaa ttcgaagccg gagtttacat caataatcgt ttaattgcca aaggtaaggg
     1621 taactctaaa caaaaagccg aggagaaagc ggcaattcag gctaccaaaa tctttcaaaa
     1681 tcaggtataa tcccgcttat gttaaaaata aaattatcac ctcgggggaa aaaacatcaa
     1741 cgaacctttc gaattgtcgt tgccgaacgg cggagcaaaa ataaaggcca aaatattgac
     1801 gaaattggtt tttttactcc ccaaaccaag accatcaatt tagataaagc caaaatggat
     1861 caatggttaa aacacggggc taaaattact accggtgtcg ataaactgct taatccggcc
     1921 aaatatcccc ataaaaagaa ggtcaagcag gctaaaagtt tacctaaaac taatgaacca
     1981 actccaacaa acgcttgaat acattcttca atcaattatc gacaacccgg aaggggttga
     2041 agttgaaacg aaagaacagg acaatttcac taatctaatt atttcagctc ctaaggaaaa
     2101 aattgggcaa attatcggaa aacaagggaa aataattaag gctatcagaa tcatccttgg
     2161 tatttcttat cccaataccc ggtttaatct ggaaataaaa gaataaaccc ctgtattaac
     2221 tttctcatta cctaaaaggg ttggaccggc cgtaaaccac cccgctccgg gtcgctattt
     2281 gctcctgagc caaactcaat atagtccgat tgttagtata atttgacaaa gtcttgagca
     2341 tctccgcttc atttttatcc aactttaaat cagccaaggt caatctctta atttccggtt
     2401 tagtttcttc gtctaaataa taagccgaaa tctccaaatc catctccgcc acttcatttc
     2461 taactctcat attcatcttc tcaattgata aaattggcag gcttttttcc agaccatcaa
     2521 gaaaatttaa ataacttgtt tttggaccaa tcaaattcaa aatcaccggc acccgtttgt
     2581 taacagcggc ttggccactt tctttttcat ctttagtcaa tttacccgga gataaagaaa
     2641 aaccttgaac agtaaagcta tatttatcgg cgatttgacg gataactcct actaaaaaat
     2701 aagcatcctt ttcataaagc aaggcgctat taagaagaga ttcatttttc ttcaaatcct
     2761 ccggcggcag actgttaata taatttattt tatcaactac catctgtttt tcggcattaa
     2821 ctttattaat ttcctgtttc agccgaccga tatcggcaat tttaggataa accccccaat
     2881 taaaaagcat aattagaaga atgataatca cgatgggttt aaccaacagc ttggctaaag
     2941 atgaatccaa accaaaaatt ttatcaggaa gagtgactgt cattattctg tttttacctc
     3001 caaactaaat ttccactggc cgctctccca tgataaactg gtaagcttgg ccgctgtaaa
     3061 acctttaact tttttattgt tgatttccgc cactttttct tcaacaatat ccatattttc
     3121 tcccttattg gtcgtataat ttatttgaaa agcattatga ccttggattt taaaatcgct
     3181 gatagtaatc tccgacggca gtaaattatt aacattagta atcgcttcac cgtactcaaa
     3241 cctcgccgcc aagactttac ccaccatttt cgcctgatat ttcaaccgat ggctggttaa
     3301 taagtcctcg ccacgatttt tatactcagc cgccacttga ttataagccg tttgaatcac
     3361 ttttaactgc cgctggcgca ccagccacca accccaaatc aaaatcgaag ctgtcagcca
     3421 aattcctgtc aaagtaacca tcagaatcaa cagttggttt tttaattttg tcttggctag
     3481 ttgaaatttt gcctgcgatg gcaggagact aacactcatt ttattctaca attcctctta
     3541 gggctaaacc aacggcataa ctgaaacgac agccctcggt gttaagatta aaagggaaat
     3601 tgattttagt cgtatcaata tttaaaaatg gttggactat ctgaacttcc actcctaaaa
     3661 ctttggttaa ttcttcggct aatccgggca tattagcccc gccgccggaa agaatcaaaa
     3721 gttcggctgg ccggccttga tcctccaaaa aaagcgacag gccttttcta atttcttcgg
     3781 ccatattcat aaaaatcggc ataatggcat ttctgatttt accttccaat tggtcctccc
     3841 gcaggccata ggctttttta tattcttcgg cgctgggcat atctaaacct aagttaatcg
     3901 aaatcgcccg ggtaaccgat tcgccgccga taggcacgga gcgggtaaaa caaatattac
     3961 cgttattagt gccgataata tcaaaaaact tttccccata atccaaggta ataatacccg
     4021 aaactgaagg gttaattgaa tttttaatta ctcgcctcat cgccaccgct ggcgactcca
     4081 aagcaattaa ctcaattcct actagtcgaa ataaatgaat aaaagcatta attaaatcgg
     4141 ttttggccgc cactacaaac actttcaacc ggctgttgtc atctctttcc acaatctcat
     4201 aatcaattgt gacttgatca agagggtagg gaacaaaggt ttcggcttca aatctaaggg
     4261 catccataat ttcattttct tttaaaggcg gcaaattgac taatcggctg acgacttcgt
     4321 cttcgggaat acaggcgacg gcctgacggg ctcggatttt taaatccgct aacaaccttc
     4381 taatcgccgc cgccatttgt ttaattccgt cagacttagc ccccttttta acccaatcgt
     4441 catttggccc tttggtttcg ccaatagcat ccaaaattaa cccattagcc gttttcgata
     4501 acgacacggc cttaatcatt cctactccta gatccaagcc aacaagatta ctcatataaa
     4561 cagtataatt agtaattagt aattagtaaa cagtaaaaat caaaaatggc ggtgattaaa
     4621 gatttttatc agctagatac atggaaggta aattataaat ttgtcttgga aatttataag
     4681 tgtttagatt cattacctaa agaagaaaaa tatggaatag ttgatcagct tagaagagca
     4741 tcgagttcaa taacagcgaa tattgccgaa ggttttggaa gatatcacaa gaaggataaa
     4801 attcattttt attttcaagc cagaggttca ttaaaagagg ttcaaaattt tctactactg
     4861 tctaaagact taggttatat taatagacag atagctagaa agttatggat aagttcaaaa
     4921 gaggcggaaa agttaattaa cggtcttatt cgctcaacag aaaacaatat gaaatcttca
     4981 taatttacta attactaatt actaattact agttactagt tactaattac taattactag
     5041 ttactaacac ttccttccgc gctgattgcc tccagcaaaa attccggcac ctgatactga
     5101 ttgacaatac gaactgtcct at
//
LOCUS       DAOOIE010000024         4769 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_14092_length_4769_cov_2.819898, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000024 DAOOIE010000000
VERSION     DAOOIE010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4769
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14092_length_4769_cov_2.819898"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="PK131_01210"
     CDS             complement(<1..1219)
                     /locus_tag="PK131_01210"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HPD44782.1"
                     /translation="MKARQLKEKYFDFFKAKGHQEIPNVSLIPENDSSTLFISAGMHP
                     LVPYLMGQPHPLGKRLVGLQRCLRTSDINSVGDGRHHIFFEMLGNWSLNDYFKEEAIS
                     WSFEFLTQILKFNPRFIWVTVYQSDKETPKIWKKLGIPENHLVTLGKKTNWWEREGVA
                     GPGGPDTEMFIDLQSNQPEVPFVIGEKSGRYLEIWNDVFMDHYTDGQGHYRDLGLKNV
                     DTGMGVERILAALDGKNNDYLTSVWQPIIKTIEVLRKGENQSLNSVQIIADYLKAVVF
                     LIADGIEPSNKEGGYVLRRLIRKSVRLAKSLAIESLTPIVQAVIDNRDNYADNYDELN
                     NPDIINVVQDEEKRFKRTLELGLKELKHYQTLTGKEAFKIYESYGFPLELIEEELGIV
                     IDKDEFDQAGKEHQ"
     gene            complement(1270..1497)
                     /locus_tag="PK131_01215"
     CDS             complement(1270..1497)
                     /locus_tag="PK131_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44783.1"
                     /translation="MDAASIILIISLIAVTVVIVVAGIYLIGILKDVKEVTSLVTVAS
                     NLFSVGGKFLTKFLESLPSVKFNSQPKKIKK"
     gene            complement(1518..2936)
                     /gene="purF"
                     /locus_tag="PK131_01220"
     CDS             complement(1518..2936)
                     /gene="purF"
                     /locus_tag="PK131_01220"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPD44784.1"
                     /translation="MCGIIGLIAPGRQVSFDLYNGLLNLQHRGQDNAGIFTTDENQVY
                     LVKGRGLVAEVFNWEKVEGLKGSMGIGQVRYPTTAGKLDSQPFYLDQGGIALAHNGNI
                     YNEKSLRQRLEDSRIYCDSDCDAETILKVFAYYFNRKTGPIAQRCFAAAKKTMQKLDG
                     SYSVVAVIRNAGFFAFRDPHAIRPFVWGKKTWNGRVKAYAFASESVAIESLVDSVSNV
                     RPGEAIFISKNLDCQRKILNRKKRAHCFFEWVYFSRSTSIIENISVNRVRWNLGKVLA
                     EIYSQSPLYERLSRGKRQVIVAPVPETARPASLAFAQRLKYQYKDILEKNRFIGRVFL
                     KPNEAIRRQEIAGNMRIIEGVVKDKIVLLIDDSIVRGTTSRGIVQRIRSRGAKEVHLL
                     STCPPLIYPCFYGVDFPDPAELVAYKRKITEIEAYIGADSITYMTLAGLTKAIGLKEN
                     QLCTACLTGRYPTSLDQLVESL"
     gene            complement(3064..3663)
                     /locus_tag="PK131_01225"
     CDS             complement(3064..3663)
                     /locus_tag="PK131_01225"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPD44785.1"
                     /translation="MYRAAGILTTTRLEKELTLEEIARKTKIPVKYLQAFETEDKKNF
                     PAEPYCSLMVKDYAQYLGLNGDYILSLFHRDFDVKTKPASWRREKFSFTPQFTFICVI
                     YIGIFLFAAYLIFEYYRYNSPPPLKINWPSLPVASETVEISGETADDATVKINGDLVV
                     VDEKGHFGKSLNYPPKDNKIVVEAQSVSGKVSRDEFVFK"
     gene            complement(3726..>4769)
                     /locus_tag="PK131_01230"
     CDS             complement(3726..>4769)
                     /locus_tag="PK131_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HPD44786.1"
                     /translation="KIMVPLGLDVAGRPKVADIAKMPHVLIAGQTNSGKSVCINSWIC
                     TLLFRASPEEVKLILVDPKRVELTPYNDIPHLLQPVIVEPEKIISALKWTIKEMENRY
                     RQLTEAGVRNIETLNEKAGFVSMPYILIIIDELNDIMMASPTEVEEALCRIAQMARAV
                     GIHLILSTQHPSVNVITGLIKANIPTRIAFAVTSMMDSRVILDLPGAEKLLGRGDMLY
                     IPPELAKPIRVQGCFVSDKERDKLIEFVKSQQQPNYNNEIVSQPVTGKGTAGKNIVSV
                     DGEERDALFDQAVKLIAESGQASSSFLQRRLNLGYARAARVIDQLEKAGIVGPSKGAK
                     PRDILIPHRQIRS"
BASE COUNT         1484 a         1078 c          833 g         1374 t
ORIGIN      
        1 tctgatgttc ctttccagct tgatcaaatt catctttatc aataactata cccaattctt
       61 cttcgattaa ctctaaagga aagccataac tttcataaat tttaaaggct tccttgcccg
      121 tcaaagtctg gtaatgtttt aattctttca aacctaattc taaggtcctt ttaaatctct
      181 tttcttcatc ctggactacg ttgataatat cgggattatt taattcatcg tagttatctg
      241 cataattatc acgattatca ataacagcct ggacgattgg cgttaaactt tcaatagcca
      301 aacttttggc taatcggact gattttcgaa ttaaacggcg aaggacataa ccgccttctt
      361 tattacttgg ttcaatgcca tcggcaatga gaaatactac cgcttttaaa taatcagcta
      421 tgatttgaac cgaatttaac gactgatttt cccctttcct taagacttcg attgtcttaa
      481 taatcggttg ccaaacagaa gttaaataat cattattttt cccatctaaa gcggctaaaa
      541 tccgttcgac ccccatgccg gtatcaacat ttttcagacc aagatctcga taatgacctt
      601 ggccatcagt ataatgatcc ataaaaacat cgttccaaat ttcaaggtag cggccagatt
      661 tttccccgat cacaaaggga acctcaggct gatttgactg aagatcaata aacatctcgg
      721 tatcggggcc gcctggaccg gctacacctt cccgctccca ccaattagtt ttttttccca
      781 gagttactaa atgattttcg ggtattccta attttttcca aatttttggt gtttctttat
      841 cagattgata aaccgttacc caaataaagc gaggattaaa ctttaaaatt tgggttaaaa
      901 attcaaaact ccaactgata gcctcttctt taaaataatc gtttaaagac caattgccta
      961 acatttcaaa aaagatatgg tgtcgaccgt caccgacgct attaatatcg cttgtccgta
     1021 aacaccgctg gagacccact aaacgcttgc ctaaaggatg gggttgaccc attaaataag
     1081 gcaccagagg atgcatgccg gcggaaataa aaagagtaga agaatcgttc tcggggataa
     1141 gggaaacatt gggaatttcc tgatgacctt tggctttaaa aaaatcaaaa tacttctctt
     1201 ttaactgccg agctttcatg agggcattat accgtatttt tcctctttaa taaaagcaat
     1261 tagttaaact tacttcttaa ttttcttagg ttgagagtta aatttaaccg agggtaaaga
     1321 ttccaaaaac ttggttaaaa atttgccgcc gacgctgaat aaattggaag ctactgttac
     1381 taagctggtt acctctttga catccttcaa aatcccaatc agataaatac cagccaccac
     1441 aatcaccacc gtgacggcaa tcaagctaat aattaaaata atcgaagcgg catccatctt
     1501 actttagtat aaactaatta aagagattcg acaagctgat caagagacgt cggataacgg
     1561 ccggttaaac aggccgtaca taactgattt tcttttaaac caatcgcctt agttaaaccg
     1621 gctaaggtca tataagtaat actgtcggcc ccaatatacg cttcaatttc ggttattttc
     1681 cgtttataag ctactaattc ggctggatcg ggaaaatcga ctccataaaa acaaggataa
     1741 atcaacgggg gacaagttga taataaatga acttctttag cccctcggct cctgattctc
     1801 tgaacaatac cccggctggt tgttccccga acaatcgaat catctatcag caaaacaatc
     1861 ttatctttaa ccacaccttc aataattctc atattaccgg caatttcttg gcgacgaata
     1921 gcttcattag gcttaagaaa aacccgaccg ataaaacgat ttttctccaa aatatcttta
     1981 tattgatact tcagccgctg agcaaaagct aaactggccg ggcgggcagt ttccggcact
     2041 ggcgccacaa taacctgccg ttttccccgt gataatctct catatagagg actttgactg
     2101 taaatttccg ccagtacttt ccctaaattc caccttactc gattgacgct aatattttca
     2161 ataattgagg ttgatcggga aaaataaacc cattcaaaaa aacaatgggc ccgttttttc
     2221 cggtttaaaa tttttctctg gcaatctaaa tttttgctga taaaaatagc ttcgccgggc
     2281 ctgacgttgc taaccgaatc aaccaaactc tcgatagcca ccgattctga agcaaaggca
     2341 taagctttaa cccgtccatt ccaggttttc ttcccccaaa caaaagggcg aattgcatgg
     2401 ggatcacgaa aagcaaaaaa accggcattg cggataaccg ccaccaccga ataagaacca
     2461 tccagttttt gcatggtttt tttagcagcg gcaaaacagc gttgggctat tggcccagtt
     2521 ttacgattga aatagtaggc aaaaaccttc aaaattgttt cggcatcaca atcagaatcg
     2581 caataaatcc ggctgtcttc aagccgctgc cgcagtgatt tctcattata aatattgcca
     2641 ttgtgagcta aggcaattcc tccctgatcg agataaaaag gctgagaatc cagcttgccg
     2701 gcagtggtcg gataacgcac ttgaccgata cccattgacc cctttaaacc ttcaactttt
     2761 tcccaattaa aaacttctgc tactagtcct cgacctttga ctaaatagac ttggttttca
     2821 tcagtagtaa aaataccagc gttatcctga ccccggtgct gaagatttag taagccgtta
     2881 tacaggtcaa aactgacctg ccgacccgga gcgatgagac caataatgcc acacatgagt
     2941 caattataac ccctcccctt tcaccaatct caattgaatt tattttcctt tctttaccca
     3001 taaattattt attttctcaa ttactaacat agaatagttg ggggcaaaat aataatatac
     3061 cggttattta aaaacaaact catcccggga gactttcccg gaaacagatt gggcctcaac
     3121 cacaatttta ttgtcttttg gtggataatt taaactctta ccaaaatgac ctttttcatc
     3181 aacaactacc aagtcaccat taatcttgac cgtagcatca tcagccgttt ctcccgatat
     3241 ttcaaccgtt tcactagcaa ccggcaaaga cggccaattg atctttaaag gcggcggcga
     3301 attatagcga taatattcaa aaattaaata agcggcaaaa aggaaaatgc cgatataaat
     3361 aacacaaata aaagtgaatt ggggtgtaaa gctgaacttc tcccggcgcc atgaggccgg
     3421 cttggtttta acgtcaaaat cgcggtggaa aagggacaaa atatagtcac cgttcagacc
     3481 taaatactgg gcataatctt tcaccatcaa cgagcagtag ggttcggctg gaaaattttt
     3541 tttatcttca gtttcaaaag cctgaaggta tttgactgga attttcgttt tccgggcaat
     3601 ttcctccaga gttaattcct tctccaagcg ggtagtagtc aaaatgccag cagctcgata
     3661 catacaattt taattcaaaa tttaaaattt ttaatatcta tgatcagcct gccggacagg
     3721 cagatctatg atctaatttg gcgatgggga atcaaaatat cccgaggttt ggctcctttc
     3781 gacgggccaa caatacctgc tttttccagt tgatcaataa cccgagccgc ccgggcataa
     3841 cccaagttaa gtcggcgctg taaaaatgat gaggaagctt gaccactctc agcaatcaat
     3901 ttaacggctt gatcaaaaag agcatccctc tcctcaccat caaccgaaac aatatttttg
     3961 ccggctgtac ccttaccggt aactggttgg ctgacaatct cattgttata attcggttgt
     4021 tgttgagatt tgacaaactc aattaattta tcccgttctt tatcagaaac aaagcaaccc
     4081 tgaacccgaa ttggtttagc caattcaggg ggaatataca acatatcacc ccggccaagt
     4141 aacttttccg ccccgggtaa atcaagaatt acccgagaat ccatcattga agttaccgca
     4201 aaagcaattc gggtaggaat attagcctta attagacccg taatcacatt aaccgacgga
     4261 tgttgggtag aaagaatcaa atgaattcca accgctcggg ccatctgggc aattcgacaa
     4321 agagcctctt cgacttctgt cggtgaagcc atcataatat catttaactc atcaatgatg
     4381 ataagaatat aaggcataga aacaaaaccg gctttttcat ttaaagtctc aatattacga
     4441 acaccagcct cggtaagttg acgataacgg ttttccattt ctttaattgt ccattttagg
     4501 gctgaaataa ttttttccgg ctcaacaatt accggttgaa gcaaatgagg aatatcatta
     4561 taaggtgtta actcaactct cttgggatca actaaaatca gctttacttc ctctggactg
     4621 gctcgaaaaa gaagggtgca aatccaagaa ttaatacaaa ctgatttacc cgaattagtt
     4681 tgaccggcaa tcaaaacgtg gggcattttg gcaatatcag ccaccttggg tctaccagcc
     4741 acgtctaaac ctaacggaac cataatttt
//
LOCUS       DAOOIE010000025         4671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_14472_length_4671_cov_3.236568, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000025 DAOOIE010000000
VERSION     DAOOIE010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4671
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14472_length_4671_cov_3.236568"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..1266
                     /locus_tag="PK131_01235"
     CDS             181..1266
                     /locus_tag="PK131_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44787.1"
                     /translation="MSEINLLTNYKPKFNYFPLLSISGISLLSLGGIILLKSKTKAAL
                     PVTNLNQPPQIDLQPTQVPKSIQHYLLASQQYFSQALTAPDQNNKINLINQSILTATE
                     AIKNFPNDYRGYHQRGRIYQSLIGNQPEFLDQCLTDLSAAFKLNSSSAEITHDLATVY
                     AQKGDAINTINYLIQTVALEPTKAQNFYDLAKIQQQAGYFADALATYQQLLTLVSDPN
                     QKQQLEIEKTALEKIVASNPRQPSSISPIDNQQQVYDPNSPLIQSFLPNHSLIIAAPE
                     TIKEIKVKNQTDSNSLSGNSYLPAGQTTITIKNSQIINDSQIYVVTIKGGKNQNLRVL
                     SKTTGSFTVGFDSPITEDVEFKWWIIN"
     gene            complement(1318..3333)
                     /locus_tag="PK131_01240"
     CDS             complement(1318..3333)
                     /locus_tag="PK131_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44788.1"
                     /translation="MTRILKYYVRIFSLLFPLFFLPIILDPYGTGKVWFLLTGGLIGL
                     VLWLIDLLIRKDGLVKVNRYLWLGLIFLIWVFIGWWRLPLGARMASFMSPLGLGMWLS
                     LMIWLFLWIQIADRDEFKIQVNWMAITGVILAVLSLITFLIPNSKLPINWPNKDNPLI
                     SIGQGWSLTGSLYGEVVFFLILGIEYLRRLLDKLKKKEKYILEAVLTGILGLALFLGI
                     FKIITFGWVSLDWRSSWVIAVETLKGGVVKNSALFGVGPGNFGTAFSLFRPASYNLTK
                     YWANAFGLSSMGLLHLWTELGIIGFLLALWGVFSLVRKWRTKTSWPVLLVVILTIFLP
                     LNLVVLFILFWLLAYKSGEIKEIKMVLNVGESGFNILPYLVSLTLMVGIVFGIYWQIK
                     VLAADYTMRQSLLAAAKNDGTGTYNLQIKAIALNPYVTSYHRLYSQTNLAIAAGMLQN
                     KDLSDDDKQKASQLIQQAVDQGKAAITLNGLESANWLNLAVIYKQLIGVVDGTADWSF
                     QAYQQAVILDPVNPLTRLDMGGLLFAAGRYDEAGRVFEQVVRDKPDFANGWYNLAYAD
                     KAQNKLAVAIADLQQAINLVPAGSGDYEKASQELEVWKKELEATTKKQGEEKSKEPET
                     LIAPEPLPTVSEEEKVEVPVDQIQPPEITPMPTATEEPAETPVIPQE"
     gene            complement(3480..4586)
                     /locus_tag="PK131_01245"
     CDS             complement(3480..4586)
                     /locus_tag="PK131_01245"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPD44789.1"
                     /translation="MGFLNRRYWEIEKRLWREFWEEETKFFTNLEKRLLGWMIAIKNW
                     ITKLMYRQRGRFSQSFINTSMAILAFLAIAFSSRVEEIIRKKDSGEGMGSNYLIMAAE
                     GIGGADTLISDLPKGEIIEYRVEEGDTLSSIAQKFGVSLDTIIWENNLKSVDAIKPRQ
                     IIRILPVTGVRYKVSRGETIHSIAKKFQVDPQNIIDYPFNSFANDETFALIAGQELII
                     PDGIKPKEVVVDTRRYVAQTVAPIPGVVGEGNFIWPTNGRISQRFSWYHPGVDIANSG
                     GPIINASQRGTVVTVGWDGRGYGNYIIINHGNGYQTLYGHLASGSIIVSPGTEVGQGQ
                     KLGNMGSTGRSTGTHLHFEIIGPKNKLNPLTVLK"
BASE COUNT         1507 a         1123 c          689 g         1352 t
ORIGIN      
        1 aaagaataaa tctgtaccga caataacgga ggcctctgaa tccgcagaag ccagcgcttc
       61 ggctactccc actcccagct ttactattgc cattgatcaa gctctcgata aagatgtcca
      121 agttaattgg tggatcatta attaaaattt ctgatttata atttttaaaa tattaaaaca
      181 atgtctgaaa taaatcttct cactaattac aaaccaaaat ttaattactt tcctctactt
      241 tctatttccg gtatcagcct tttatcttta ggtggaatta ttctattgaa gtcaaaaact
      301 aaagccgcct tgcctgtcac taatctaaac caaccaccac aaattgatct ccagccaact
      361 caagtgccta aaagtatcca gcattattta cttgcttctc agcaatattt ttctcaggcg
      421 ttaactgctc ctgatcaaaa caataaaatt aatctaatta atcaatccat tctaacagcg
      481 accgaggcaa ttaaaaattt tcccaatgat tatcgtggct atcaccaacg gggccggatt
      541 taccagagtt taatcggcaa tcagccagaa ttcctcgacc aatgtttaac tgacttatca
      601 gccgccttca aattaaattc cagttcagcc gaaattaccc atgatctagc caccgtctat
      661 gctcaaaagg gcgatgctat taataccatt aattatctaa tccaaactgt tgctttagaa
      721 ccaaccaagg ctcaaaattt ttacgattta gctaaaattc aacaacaggc cggttatttt
      781 gccgatgctt tggctactta tcagcaatta ctaaccttag tcagcgaccc caatcaaaaa
      841 caacaattgg agattgaaaa aacagcctta gaaaagattg ttgcctccaa tcctcgccaa
      901 ccatcttcta ttagtcccat cgacaaccaa caacaagtct acgatccgaa ttctccctta
      961 atccaatctt tcctgccaaa ccacagtttg attattgccg ctccggaaac aatcaaagaa
     1021 atcaaagtta aaaaccagac tgattctaat tccctctctg gtaattccta tctacctgcc
     1081 ggtcaaacaa ctattaccat taaaaattcc caaatcatta atgattcgca aatctatgtc
     1141 gttactatca agggcggaaa aaaccaaaat ctcagggtct tatcaaaaac gactggctct
     1201 tttactgttg gattcgactc ccccatcact gaagatgtcg aattcaaatg gtggatcatt
     1261 aactaaaatc caataaccaa attccaagaa acaaactttc tcatttgtaa ttcaccctca
     1321 ctcctgtggt attaccggtg tttccgctgg ttcttcagtg gctgtcggca tcggtgttat
     1381 ttccggcggt tgaatttgat ctaccggcac ttctactttt tcctcttcac taactgttgg
     1441 taatggctcc ggcgcgatta atgtctccgg ctctttagac ttctcctctc cctgcttttt
     1501 agttgtcgct tctaattcct tcttccaaac ctctaattcc tgactggctt tttcataatc
     1561 accactacca gctggcacta aattaattgc ctgctggaga tcggcgatag ccactgccag
     1621 tttattttga gccttatccg cgtaagccaa attataccaa ccattagcaa aatcaggttt
     1681 gtctcgaact acttgctcga aaactcttcc ggcttcgtca tagcggccgg cggcaaataa
     1741 aaggccaccc atatccaatc tggtcaaggg attaactggg tctaaaataa ctgcctgttg
     1801 ataagcttga aaggaccaat cggcagtgcc gtcaaccacc ccaatcaact gcttataaat
     1861 aacagctaaa tttagccaat tggctgattc cagaccgttt aaagtaatag ccgccttacc
     1921 ctgatcaacc gcttgttgaa tcaattgcga tgctttttgc ttatcatcat cacttagatc
     1981 tttattctgg agcatccctg ccgcaattgc taaatttgtc tgagagtaaa gccgatgata
     2041 actcgtaaca taggggttaa gagcaattgc ttttatttgt aaattatatg tccctgtacc
     2101 atcattttta gctgctgcca gcagtgactg ccgcatagta taatcggccg ctaaaacctt
     2161 tatttgccaa tagataccaa agacaattcc taccattaaa gttaaggaaa ccaaataggg
     2221 taaaatatta aagccagact cccccacatt taaaaccatt ttgatttctt ttatttctcc
     2281 tgatttatag gctaaaagcc aaaagagaat aaacaaaacc actaaattta aaggtaaaaa
     2341 gatcgttagg ataactacta gtaaaactgg ccaacttgtc ttagttcgcc attttctcac
     2401 caaactaaaa actccccata aggctaaaag aaagccaata atacctaatt cagtccagag
     2461 atgcaacaat cccattgacg ataaaccaaa ggcattagcc caatatttgg ttaaattata
     2521 agaggccggt ctaaataaac tgaaggcagt accaaaattc cccggaccaa ccccaaacaa
     2581 agcactattt ttaaccaccc cgccctttag ggtctccacg gcaattaccc aagaagaacg
     2641 ccaatccaaa ctaacccagc caaaagtaat tattttgaaa atccctaaaa acaaagccaa
     2701 acccaaaatg cccgtaagaa ccgcttctaa aatatatttt tcctttttct ttagtttatc
     2761 caaaagtctt cttagatatt caattcccaa aattaaaaag aaaactacct ctccgtaaag
     2821 agaacctgtt aaggaccagc cctggccaat agaaatcaag ggattatctt tattcggcca
     2881 attgatcggc aatttactat tgggaataag aaaggttatt aaggataaaa cagccaaaat
     2941 cacccctgtt attgccatcc aattaacctg gattttaaat tcgtcccgat cagctatctg
     3001 aatccaaaga aatagccaga tcattaagga cagccacata cctagaccca acggggacat
     3061 aaaagaggcc attcttgccc ctagcggtaa tctccaccaa ccaataaata cccaaatcaa
     3121 aaaaattaat cccagccaaa gataacgatt aacttttact aaaccatctt tcctaattaa
     3181 tagatctata agccacaaaa ccaaaccaat caatcctcct gttagtaaaa accacacctt
     3241 accggtacca tacggatcaa gtataatcgg gagaaagaat aacggaaata atagactaaa
     3301 aatacgaaca taatatttta aaattcgagt catggttcat gatagcaaaa tcatttttta
     3361 tcgacaatat atcaaactac ttattaaccg cattatttcc taaaatgtca tagacctgta
     3421 gagacatcat ccatttattc tcttcttttt cgaagagggg gttaagggaa aattttgttt
     3481 tattttagaa cagtcaaggg atttaattta tttttcggtc caataatctc gaaatgcaaa
     3541 tgggtacctg tcgaccttcc ggtgcttccc atattaccca gcttttgtcc ctgaccaact
     3601 tcggttcccg gactaacaat aatagagccg ctagctaaat gaccataaag ggtttgataa
     3661 ccgttgccgt ggttaataat aatgtaatta ccataaccac ggccatccca acctaccgtg
     3721 actactgttc ccctttggga agcattaatt atcggtccgc ctgaattagc gatatcaacc
     3781 cctggatgat accaagaaaa acgctgggaa attcgaccat tagttggcca aataaaatta
     3841 ccctcaccaa ccactccagg aattggagct acagtttgag cgacatacct tctagtatca
     3901 accacgactt ccttaggttt aatcccgtcg ggaataatta attcttgtcc agcaattaga
     3961 gcaaaagttt catcattagc aaaggaatta aaagggtaat caataatatt ttgaggatca
     4021 acttgaaact ttttggcaat actgtggata gtttctcccc gggaaacttt ataacgtacc
     4081 ccagtcaccg gtagaattct aattatctgc ctaggcttaa tagcatcaac cgacttcaaa
     4141 ttattctccc aaataatcgt atctagagaa acaccaaatt tctgggcaat tgaagacaag
     4201 gtatctccct cctccactcg atactcgata atttctcctt taggtaaatc agaaataaga
     4261 gtatcggccc caccaatacc ttcagccgcc ataatcaaat agttactacc cattccctcg
     4321 ccactatctt tctttctgat aatttcctct actctactag agaaagcgat agccaaaaag
     4381 gccaaaatcg ccatactggt attgataaaa ctttgggaga aacgacctct ttgccgatac
     4441 ataagttttg ttatccaatt cttaatagct atcatccaac ctaaaagtct tttttccaga
     4501 ttggtaaaaa atttagtttc ttcctcccaa aactctcgcc atagcctctt ttctatttcc
     4561 caatatcgtc ggtttaaaaa acccatattt attttcctac ctttttcaaa aactcctcat
     4621 tgtctcttgt ttttctcatt tgctcaataa tcatctcggt ggcaccatca t
//
LOCUS       DAOOIE010000026         4650 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_14560_length_4650_cov_3.249184, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000026 DAOOIE010000000
VERSION     DAOOIE010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14560_length_4650_cov_3.249184"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="PK131_01250"
     CDS             <1..233
                     /locus_tag="PK131_01250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD44790.1"
                     /translation="MEAVACGTPVIASNRGGLPEALDNSVAILIKPTPTNLVKAIKKI
                     RKKYSVYQKNCRPYALKNYSNKNLTLITRFYK"
     gene            complement(210..785)
                     /locus_tag="PK131_01255"
     CDS             complement(210..785)
                     /locus_tag="PK131_01255"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPD44791.1"
                     /translation="MMKVNLGCGPSGKTKGWLNYDWGLLPLLNKMGLLKVAISFGLID
                     KSYLVKWPKFHLVDIRKPLPLKDHSVDFIYCSHVLEHFEPAVIRKVLLECRRVLKNNG
                     VLRIVVPDIKYIINNYAGAKRFNEFVYGYDKEKPYRFRNFIRGHQWLFDREELEEKLR
                     EAGFQTIKFTNIKKIDLPQYKGNSLFVEASN"
     gene            800..1912
                     /locus_tag="PK131_01260"
     CDS             800..1912
                     /locus_tag="PK131_01260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPD44792.1"
                     /translation="MKQILFIANNNIGRGQSGGDTIFLNFAKYWPGVTIIGSAETRNL
                     MGRYHLRNQFIQTDKASHLPLFFHQIRRTILGILKIKNLKFFNYVYSVSDFYPDLLPA
                     IWYKLKHPQTKLICGYYLLAPNPFSHSSPYRQTGQFLKGLFYWLSQRLSLFLVNRYAD
                     IVLITSEPDRKYFPNKKVIVVQGGVDLPAKIKSTKIYDAVFIGRLHPQKGILELIDIW
                     QKVVGQRPNAKLAVIGDGSLERNLKFKIQKLKLKNNITLFGFKTGKEKYQIFSQSKIV
                     VHPAIYDSGGMAPAEAMSLGLPGVAFDLEALKSYYPQGMLKTKCFDQNLFAQNILKLL
                     QDKRLYRRLSQEALTLINRYWLWPQRAQKIYENLIY"
     gene            1893..3008
                     /locus_tag="PK131_01265"
     CDS             1893..3008
                     /locus_tag="PK131_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44793.1"
                     /translation="MKILFIEPCFKNFGGYFRSINICRSLAKKNIKVTLLISSDQKFS
                     PIIKKTIINRNLTQYELPRWEINFYLNGRILRGLIATVWGLIGRFDIIHAAVPAQLES
                     NIPALILRLSGKKVVFDWDDLLENEISISPFLRSYSRFCEKFFPKIIKNYVVVSDLLF
                     NLAQCRGATKIIKIINGVDVHQFKLYPQKKNYNFLTFGNTYSRDRTRLLLLLFEKIWR
                     QEPRAQLIWNLDPLKLVQQQKLKINPKIINNIHSVGNIPQKNLGKYIAQSQAILFMMG
                     NLDSERACFPIRIGSYLSGEAIIILNDTNTEVVKTLKHYQCAIIDKDLDILASKSVEL
                     LHNPKIRQKLQPKINRAKSELSWDNLITPLINFYKTL"
     gene            3005..4072
                     /locus_tag="PK131_01270"
     CDS             3005..4072
                     /locus_tag="PK131_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007309636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPD44794.1"
                     /translation="MKSGYVLITGSGGLIGSEAAKFFADKGFKIIGIDNNLRQYFFGP
                     EASTQWNQTELQKKLKNNYLYFNIDIRDEKSIEKIFKKYRFDLIIHTAAQPSHDWAAK
                     EPLTDFTVNALGTLILLENYRKYCPKAVFIYTSTNKVYGDTPNKLPLIELKTRYEIDP
                     KHPFAQNGIDESMSIDNSTHSLFGVSKASADLMVQEYGKYFGLPTTVFRGGCLTGPSH
                     SATKLHGFLAYLIKCIAEGKKYTIFGYKGKQVRDNIHSFDLVNAFYQVYKKPRCGEVY
                     NIGGSRHSHISMMEAIFKVESALGKFALIEYIDTPRIGDHIWYVSDVSKFKKHYPNWN
                     YQYNIDDIIKEICELGHFRQQ"
     gene            4069..>4650
                     /locus_tag="PK131_01275"
     CDS             4069..>4650
                     /locus_tag="PK131_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPD44795.1"
                     /translation="MKYKNILITGGAGFVGSNLAISLKQKYPQTKITCLDNLSRRGSE
                     LNLTRLKQNKIVFNYGDIRQPEDLNFLTADLIIDCSAEPSVLAGITNPYFLINNNLIG
                     TINCLELARIRKSDFIFLSSSRVYSIKELNRQKFKAVAENFPLGRVRSLYGTTKLAAE
                     LIIQEYADSFNLKAIINRCGCLAGPWQFGKVDQG"
BASE COUNT         1502 a          927 c          754 g         1467 t
ORIGIN      
        1 ccatggaagc agttgcttgc ggcacgccgg taattgccag taaccgcggc ggtttacccg
       61 aagctttgga caattcagtc gctattttaa tcaaacctac tccaaccaat ctcgttaagg
      121 ccattaaaaa aattcggaaa aaatattcgg tatatcaaaa aaactgccgt ccttacgctc
      181 taaaaaatta ctcaaataaa aatttaactc taattactcg cttctacaaa taaactattc
      241 cctttgtatt ggggtaaatc gatcttctta atatttgtaa acttgattgt ttgaaaacca
      301 gcttcccgta atttttcttc caattcctct ctatcaaata accattgatg tccccgaata
      361 aaatttctaa atctataggg cttttcctta tcataaccat agacaaattc attaaaccgc
      421 ttggcaccag cataattatt aataatatat ttaatatccg gcaccactat ccgcaaaaca
      481 ccattatttt ttaaaactcg acggcattcc aataaaactt ttctgattac tgccggttca
      541 aaatgttcca aaacatggga acaataaata aaatcaaccg aatggtcttt taatggcagg
      601 ggtttcctaa tatccaccaa atgaaatttc ggccatttga ccaaataact tttatctatt
      661 aaaccgaaag atatcgcaac ttttaataac cccattttgt ttaacagtgg taataaaccc
      721 caatcgtaat taagccaccc tttagttttt cctgaaggac cacagcctaa attaactttc
      781 atcatagtac aatagagcag tgaaacaaat tttatttata gctaataata acatcggtcg
      841 aggccaaagt ggaggcgaca ctattttcct taattttgct aaatattggc ctggggtaac
      901 tattatcggc tcggcggaaa ccagaaattt aatggggcgt tatcatctcc gaaatcaatt
      961 tattcaaacc gataaagctt ctcatttacc tctttttttt catcaaatca gaagaacaat
     1021 tttgggtata ttaaaaatta aaaatttaaa attttttaac tatgtttaca gtgtttcaga
     1081 tttttacccc gatctcttgc cggctatatg gtataaatta aaacatcccc aaactaaact
     1141 tatttgcggt tactacctcc tagcccctaa cccctttagt cattcatcgc cctaccgaca
     1201 aactggtcaa tttttaaaag ggctttttta ttggttatcc caacgtttaa gtttatttct
     1261 agtcaatcgt tacgccgata tcgttttaat tacctcagag cccgatcgga aatattttcc
     1321 caataaaaag gtcatagtag tgcaaggagg agttgactta ccagccaaaa ttaaatccac
     1381 caaaatttac gacgctgttt ttataggccg actgcacccc caaaagggaa ttctggaatt
     1441 aattgatatt tggcaaaaag ttgtcggaca aagaccaaat gccaagctgg ctgttatcgg
     1501 tgatggttct ttggaaagaa atttaaaatt taaaattcaa aaattaaaat taaaaaacaa
     1561 tatcaccctg tttggattta aaaccggcaa ggaaaaatat caaatcttca gtcaaagtaa
     1621 aattgtggtt catccggcta tttatgacag tgggggaatg gcgccggcag aagctatgtc
     1681 gcttggttta ccgggagttg cttttgatct agaagctcta aaaagttatt accctcaggg
     1741 aatgttaaaa actaaatgtt ttgaccagaa cttattcgcc caaaatattt taaaactact
     1801 ccaagataag cgactttacc gtcgcttgtc ccaagaagct ctaactttaa ttaatcgtta
     1861 ttggctttgg cctcaacgag cccaaaaaat ctatgaaaat cttatttatt gaaccttgtt
     1921 ttaaaaattt cggcggttat ttccgatcga ttaatatctg ccgttcttta gcaaaaaaaa
     1981 atatcaaagt tactttgtta atttccagcg atcagaaatt ttcgccaatc attaagaaaa
     2041 ctattattaa ccgaaatttg actcaatatg aattgccccg ttgggaaatt aatttttacc
     2101 ttaatggtcg gattttgaga ggtttaatag ccacggtttg gggcctcatc ggccgctttg
     2161 atattatcca cgcggctgtc cctgcccaat tggaaagtaa tattcccgct cttattttac
     2221 gattaagtgg taaaaaggtc gtttttgatt gggatgatct tttggaaaat gagatttcaa
     2281 tctccccctt cttacgctca tactcccgct tttgtgaaaa gttttttcct aaaataatca
     2341 aaaactatgt agtggttagc gatcttcttt ttaatttagc ccagtgccgt ggtgctacta
     2401 aaattattaa gattattaat ggtgttgacg ttcatcaatt taaactttat cctcaaaaga
     2461 aaaattataa tttccttacc tttggcaata cttactcaag ggatcgaacc aggttattac
     2521 ttttattgtt cgaaaaaatt tggcggcaag aaccgcgggc tcaattaatt tggaatttag
     2581 atcctctcaa acttgttcaa caacaaaaac tcaaaattaa tcccaaaata ataaacaata
     2641 tccatagcgt cggtaatatt ccccagaaaa acctcggcaa atatattgct caatcacagg
     2701 caattctttt tatgatgggt aatttggata gcgagcgggc ttgttttcct attagaatcg
     2761 gcagttattt aagcggtgaa gcaattatca ttttaaatga taccaacacc gaagtggtca
     2821 aaactctcaa acattatcaa tgtgccatta ttgataaaga tcttgatatt ttggcttcaa
     2881 aatcagtcga actactccat aaccctaaaa ttaggcaaaa attacaacca aaaattaatc
     2941 gtgccaaaag tgaactttcg tgggataatc taatcacccc cctgattaat ttttacaaaa
     3001 ctttatgaaa tcgggatatg tcttaattac cggatcgggc ggtttaatcg gttcggaagc
     3061 ggctaaattt ttcgccgata aaggctttaa aattattggc attgataata atctgcgaca
     3121 gtactttttt gggccggaag cttcaactca atggaaccaa acagaactcc agaaaaaact
     3181 aaaaaataat tatctttact ttaatatcga tatcagagat gaaaaatcga tcgaaaaaat
     3241 atttaagaaa tatcgatttg atttaattat ccatacagca gcccaaccat cacatgattg
     3301 ggccgccaaa gaacccctga ccgactttac tgtcaatgct ctaggtacct taattttact
     3361 tgaaaattac cgaaaatatt gccccaaggc tgttttcatc tatacttcaa ctaataaggt
     3421 ttatggcgat acccccaata aactaccttt aattgaacta aaaaccagat atgaaatcga
     3481 cccaaaacat ccatttgccc aaaacggcat tgatgagtca atgagtattg ataactcaac
     3541 tcatagcctc tttggtgtct cgaaggcgtc agctgattta atggtccaag aatatggtaa
     3601 atattttggt ttaccgacaa ccgtttttcg cggcggctgc ctaaccggtc cgagtcattc
     3661 ggccaccaaa ctccatgggt ttcttgctta tttaatcaaa tgtattgctg aagggaaaaa
     3721 atacactatc ttcggttata aaggcaaaca agttcgcgac aatatccact cttttgattt
     3781 agtcaacgct ttttatcaag tctataaaaa accacgatgc ggagaggttt acaatattgg
     3841 cggctcccgc cattcccata tctccatgat ggaggcaatt tttaaagtgg aaagtgctct
     3901 gggtaagttt gctcttattg aatacatcga taccccaaga atcggcgatc atatttggta
     3961 tgtttctgat gtttctaagt ttaaaaaaca ttatcccaat tggaactatc aatacaacat
     4021 tgacgatatt ataaaggaaa tttgtgaact cggtcatttt cgtcaacaat gaaatataaa
     4081 aatattttaa ttactggcgg cgccggtttt gtcggctcta atttagctat cagtctcaaa
     4141 caaaaatacc cccaaactaa aatcacctgt ctggataatt tgtcccgccg cggctcagaa
     4201 ctaaatttaa cccgacttaa acaaaacaaa attgttttta attatggtga cattcgtcaa
     4261 cctgaagatc ttaatttttt aaccgctgat ttaataattg attgttcagc cgaaccttcg
     4321 gttttagccg gcatcaccaa cccttatttt cttattaata ataatctaat tgggactatt
     4381 aattgtcttg aattggctag aatcagaaag agcgatttta tctttttaag cagtagccga
     4441 gtttactcta ttaaagaatt aaaccggcag aaatttaaag ctgtggccga aaattttcct
     4501 cttggccggg ttcgttcact ctatggcacc actaaattgg ctgccgaatt aattatccaa
     4561 gaatatgctg actcttttaa tttaaaagcg atcatcaatc gctgtggttg tctggctggt
     4621 ccctggcaat tcggcaaagt tgatcagggc
//
LOCUS       DAOOIE010000027         4380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_15626_length_4380_cov_3.391214, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000027 DAOOIE010000000
VERSION     DAOOIE010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15626_length_4380_cov_3.391214"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PK131_01280"
     CDS             complement(<1..438)
                     /locus_tag="PK131_01280"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPD44796.1"
                     /translation="MSHLVVVDGHAVAHRAYHSIPPLTHNGQPVNALYGFYSILLSAV
                     DTLKPNYLVVCLDSPGPTFRQSEYLAYRARRQPTEHNLVIQLPQIPLTLEKAHIPSIS
                     LGGYEADDLIASLIVQHRDQIDHSTIITGDQDLMQLVDDQTNLF"
     gene            495..1403
                     /locus_tag="PK131_01285"
     CDS             495..1403
                     /locus_tag="PK131_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44797.1"
                     /translation="MAQHPIPQQISSYEFKLVGEMTLKQFFKAAAGIILALLINATKI
                     HVLLRWPLMLASAGIGLAFAFIPYQDKPLEYWLVSFFKVIFTPTIYLFQKDSSQPVAS
                     GILIEETRKTTSKEEFDGKVRPLNNKGEVKDFINNLPSVKSIKKTEKQQQEKKEEEKA
                     QKVSQMETIIDLENSLEPATEENWRDQKANLNLKTEKLQATGKAVFGKIPMPDIPEIP
                     NLVVGMVTDAMGKIIDGAIVEIQDQHGNSARVLKTNSLGQFRIASPLQDGEYLIICEY
                     ENHNFDRVNINLNGNIVQPIRIISNN"
     gene            1442..2122
                     /locus_tag="PK131_01290"
     CDS             1442..2122
                     /locus_tag="PK131_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44798.1"
                     /translation="MVNDPLKIAIKGTTQEHLPIEDIIDDIVLLKDGNCVMILQVSSV
                     NFDLLSEKEQLALVYAYGNILNSLNFPIQIVIRTKMKDISQYLSNLKNYEKQQNHPLL
                     KERIKSYQKFIEETVKRNEVLSKTFYLVIPFSVYELGIGALVPRGSKRTLDKQTVLSK
                     AKPSLETKRDHLIRLFSRIGLDIKPLNNKEIIELFYSIYNEEVADQQKIENMDYQAPI
                     IEAKLKTV"
     gene            2128..4350
                     /locus_tag="PK131_01295"
     CDS             2128..4350
                     /locus_tag="PK131_01295"
                     /inference="COORDINATES: protein motif:HMM:NF040179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD44799.1"
                     /translation="MGILDKIIKKPTTKETNPQSSNQIKQFNSGIVTTKDIIAPGALE
                     VDYDFIRIDNRYYRTLLVVSYPRFVDMGWLSPLINFDAALNVSMYIFPANGKEVLDDL
                     RRKITEMEAELNSDIKQGRVADPATQAKLEDALNLQADLVRGEERFFQFGLYITISAN
                     SINELNKINKNLEANLGALMINTKRATLRMENGFKTTLPICSDKLNILRNMDTTSLAT
                     TFPFVSSDLSDDKGVMYGINEHNGSLVIFDRFTMLNYNSVVFATAGAGKSYLVKLEAL
                     RSAMLGIDIIIIDPENEYQDLAEAIGGQYIAFGYNEESKINPLDLSLINEEGENALNV
                     KALTLQKLFKIMLGKMDPNEEAILDKAIMEAYRSKGITSDPETQKNEPPLIEDIYKVL
                     LGNENEESKNLALRLEKYIKGSFAGIFNQKTTVNLKSNFVVFGIKNLEESLRPIAMQI
                     ILDYIWTIVKKDLRKRFMIVDEAWYLMKYPDSADFLRSIVKRGRKYYLGVTTVTQDVD
                     DFLKTDFGKEIVTNSAIQILLKQHSAAIDQIGEVFYLSEGEKQLLLTADKGEGIFFAG
                     ANHVAIRVIASEEENKLITSNPEELLKIKQEKKEIAASNHLPPQESGQEVVIPLRQKV
                     EELEKNEKIKLKEFKDRENKFETLKRLTQEIDQSPLPSNLPKPIFINQTPEPKIPQPP
                     QLQKKSQPIFTKVPTSKTDNLSQPNPPKTPLAKIELVKQSAQADKSTDYDKLLGNGIV
                     "
BASE COUNT         1592 a          751 c          776 g         1261 t
ORIGIN      
        1 aaaaagattt gtttgatcgt caactaattg cattaaatcc tgatcaccgg taataatcgt
       61 cgagtgatca atttgatcac gatgttgaac tattaaactg gcaatcaaat catcagcctc
      121 ataaccacct aaacttatac tagggatatg ggctttttct agggtaagag gaatttgggg
      181 tagctgaata actaaattat gttcagttgg ctgacgtctg gcccgataag ctaaatattc
      241 tgactgtcga aaggttggac ctggagaatc aagacaaact accaaataat tcggtttaag
      301 agtatcgaca gccgaaagca atatagaata aaaaccatac aaggcattga ccggctgacc
      361 attatgggtt aaaggaggga tagaatgata agctcgatga gcaacagcat gaccatccac
      421 tactactaaa tgagacatat ctcattgtaa atcataaatt acaaatcata aataatttac
      481 gatagaatta attaatggcc caacatccaa ttccgcaaca aatttcatcc tatgaattta
      541 aattagtagg ggaaatgact ttaaaacaat tttttaaggc ggcggcgggc ataatcttgg
      601 ctcttctaat taatgccact aaaattcatg tccttttacg ttggcctctc atgttagcct
      661 cggcaggaat cggtttagct ttcgctttca tcccatatca agataaaccg ttagagtatt
      721 ggcttgtttc cttttttaaa gttattttca ccccaactat ctaccttttt caaaaagaca
      781 gttcccagcc agtggctagc ggtattttaa tagaggaaac aagaaaaaca acaagtaagg
      841 aagaatttga tggcaaggtc agacctttaa ataataaagg cgaagttaaa gattttatta
      901 acaatctacc ttcagtaaag tcaattaaaa aaaccgaaaa acaacagcaa gaaaaaaaag
      961 aagaagaaaa ggcccaaaag gtctcccaaa tggaaactat aatcgacctg gaaaatagtt
     1021 tagagccagc cactgaggaa aattggcgtg atcagaaagc taacttaaat ttaaaaacag
     1081 aaaagcttca ggcaaccggt aaggctgttt ttggtaaaat tcccatgcct gatatccccg
     1141 agattcccaa tttagttgtc ggtatggtaa ctgatgctat gggtaaaatt attgacggag
     1201 ctattgtaga aattcaagat cagcacggta attcggctcg ggtcctcaaa actaattctt
     1261 taggacaatt tagaattgcc agtcctcttc aagatgggga atatcttatt atctgtgaat
     1321 atgaaaacca taatttcgat cgggttaata ttaatcttaa cggaaatatt gttcagccga
     1381 ttagaattat tagtaataat tagtaattaa taaaaaaaac aagtattatt aatctaaaga
     1441 tatggttaat gatccgttaa aaattgctat caagggcact actcaagaac atttgccgat
     1501 tgaagatatt attgacgata ttgtcctttt aaaggatggt aactgtgtta tgattcttca
     1561 ggtttcttca gtaaattttg atcttttatc agaaaaggaa caattagccc tagtttatgc
     1621 ctatggcaat attttaaatt cacttaattt ccctattcag atagtcatcc gaaccaaaat
     1681 gaaagatatt tcccaatatt tatcaaatct taaaaattac gaaaaacaac aaaatcatcc
     1741 tcttttaaaa gagaggatta aatcttacca aaaatttatt gaagaaacgg ttaaaagaaa
     1801 tgaggtttta tctaaaacat tttatttagt gattccattt tctgtttacg aactgggtat
     1861 tggtgcttta gttcctcgag gaagtaaaag aaccttggat aagcaaaccg ttttaagtaa
     1921 ggctaaacca agcttagaaa ctaaacgaga tcatcttatc cgtctttttt cgcgaatagg
     1981 attagatatt aaaccgctaa ataataaaga aataatcgaa ttgttttata gtatctacaa
     2041 tgaggaagtg gccgatcaac agaaaattga aaatatggat taccaggcac caataattga
     2101 agctaaatta aaaactgtat aaaaactatg ggaattttag ataaaataat taaaaaacca
     2161 accacgaaag aaactaaccc tcaatcttct aatcaaataa agcagttcaa ttcgggaata
     2221 gttaccacca aagatattat cgctccagga gctttagagg tcgactacga ctttattaga
     2281 attgataatc gctattatcg caccctcctg gtagtatcct atccccgttt tgttgatatg
     2341 ggttggttat caccactgat aaactttgat gcggcattaa atgtatctat gtatatcttt
     2401 cctgccaatg gtaaagaggt tcttgatgat ttaagacgaa aaattactga aatggaagct
     2461 gaattaaatt ccgatataaa gcagggtcga gtagctgatc cggcgactca agctaaatta
     2521 gaagacgctt taaatctcca ggccgattta gttcgagggg aggaaagatt tttccaattt
     2581 ggtctataca ttaccatcag tgctaattca attaatgaat taaataaaat caataaaaat
     2641 ctagaggcta atttaggtgc tttaatgata aatactaaac gggctactct ccgaatggaa
     2701 aatggtttta aaacaacact gccgatttgc agtgataaat taaacatttt gcgcaatatg
     2761 gataccacat cgttagccac cacttttcct ttcgtctcat ctgatttatc cgatgataag
     2821 ggagtcatgt atggtattaa cgaacacaac ggttctctgg tcatttttga tcgatttacc
     2881 atgttaaatt acaattcagt tgttttcgcc actgctggtg cgggtaaatc atacttagtt
     2941 aaactagaag ctcttagatc agcaatgcta ggtatagata taattatcat cgaccccgaa
     3001 aacgaatacc aagacttagc tgaagcgatc ggcgggcaat atatcgcttt tggttataat
     3061 gaagaatcaa aaatcaatcc cctagattta tcactaatta acgaagaagg agaaaatgcc
     3121 cttaatgtca aagctttaac tttgcaaaaa ctattcaaaa ttatgctggg gaaaatggac
     3181 ccaaatgagg aggctatttt agacaaggcg ataatggaag cctatcgaag taagggtatt
     3241 accagcgacc ctgaaaccca aaaaaatgaa ccaccattaa tagaagatat ttataaagtt
     3301 ttactaggaa atgagaatga ggaatcaaaa aatttagctt taaggctgga aaaatacatt
     3361 aagggttcgt tcgctggtat ttttaatcaa aaaaccaccg tcaatcttaa aagcaacttt
     3421 gtggtttttg gtatcaaaaa tcttgaagaa agtcttcgac caatagcaat gcaaataatt
     3481 ctggattaca tctggacaat agtgaaaaag gatttgagaa aacggtttat gatagttgac
     3541 gaggcttggt atttaatgaa atatcctgac agtgccgatt ttcttagaag tattgtcaag
     3601 cgaggtcgta aatattatct tggggtgact acagtgactc aggatgtcga tgactttttg
     3661 aaaacagatt ttggtaaaga aattgtcact aatagcgcca ttcaaattct attaaaacaa
     3721 catagtgccg ccattgacca gataggtgaa gttttttacc tatcagaagg agaaaaacag
     3781 ttactattaa cagccgataa gggtgaaggt attttctttg ccggagctaa tcatgttgct
     3841 attagagtaa ttgcctcaga agaggaaaat aaattaatta cttctaatcc cgaagagcta
     3901 ttaaaaatta aacaagagaa gaaggaaata gcggcctcaa atcatctccc acctcaggaa
     3961 tctgggcagg aagtagttat tcctcttagg caaaaagttg aagagttgga aaaaaatgaa
     4021 aaaataaaac tgaaagaatt taaggatcga gagaataaat ttgaaaccct taaaaggctg
     4081 acacaggaga tagaccaatc acctctccct tcaaatttgc ctaaaccaat ttttattaat
     4141 caaacacccg agccaaaaat tccccaacca ccccagctac aaaaaaaatc tcaaccaatt
     4201 tttaccaaag tcccaacctc taaaactgat aatttatcgc aacccaatcc acctaaaaca
     4261 cctctagcta aaattgaact agtgaaacag tccgctcagg ccgataaatc aacagattac
     4321 gataaactat taggtaatgg aatagtataa tttattaatg ataattgatt tagccaaatt
//
LOCUS       DAOOIE010000028         4339 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_15822_length_4339_cov_3.447946, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000028 DAOOIE010000000
VERSION     DAOOIE010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4339
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15822_length_4339_cov_3.447946"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..649
                     /locus_tag="PK131_01300"
     CDS             59..649
                     /locus_tag="PK131_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44800.1"
                     /translation="MSQFNLTVTDIATAYERGLLPQRNNRSYFYRDTACRSNLANFSL
                     SSENRRILKKTQQFSFRKTPLKDFKFDLEIQKQIYHWTKTLKWNFPISSVKNVFTNHF
                     FNYLYDWTDNNNQSVAYAICYFSTSIAHIAYVFYNPRYNHSDLPIRLVLQVVIDSHDL
                     NLTYCYLGRFSTTTGYYKRAMPGFEYFSQGKWKKYN"
     gene            651..1841
                     /locus_tag="PK131_01305"
     CDS             651..1841
                     /locus_tag="PK131_01305"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPD44801.1"
                     /translation="MKIHILGIAGSMTTPIALYLKQQGHTISGSDQDKIYPPASDLLK
                     NIPFNQPLPSKIDLCLVGGSYNKFQRTRQEFAFIHKHHYPYISTTHFLANQLNSLNPI
                     LIAGTFGKTSISALLAWNLSQAGFQPNYFFGGIALNQFPSLAITGSDWSVIEADESIN
                     GLDTQAKFLSYPIKYLILTSAAWEHRDCYRTAKDNFEAFRQLVQRLPADGLLIANKYG
                     KNISDLIKFSPAPVIFYSGSNEEAVATFCQQFKIKFDPNFPGLKRRLELIDRRNNILI
                     FDDFAQSPDRISYTLNLLRNRYPKYNLKVLFEPHASFLRYSIANLGQALESAQEVILG
                     AITYTHDKNHRITLKDYQKEIGPKLIYLPLKTDIINYYRQTLKPGDLLIRFASAGENL
                     CLNI"
     gene            1826..3154
                     /gene="murA"
                     /locus_tag="PK131_01310"
     CDS             1826..3154
                     /gene="murA"
                     /locus_tag="PK131_01310"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPD44802.1"
                     /translation="MSQYIINGGKALKGEVSIRGAKNASFKQIIATLLTDKTCQLDNV
                     PVIADVKITESIAKQLGTQIKPVGEHSIELTTSTITSTTVPFGTGQKSRSSFLFAAPL
                     LARKGQATFPLPGGDKLGDRPLDRFFDCLKRMNVKIHENNGIITLTTTNLKATQYRFN
                     KPSHTVTEAVLLLATLTPGTSIFQNCAREPEIDDLIEFLKDMGAKINRDSKYPDQIQI
                     TGVSKLTGAKHQVIADRNEAVTFACAALATKGSVNILRINPAIIETFLQTVEQMGARV
                     DRGKEELIISWVQPLKAVDIETGPEPMFMTDWQPVFTVVLSQAIGCSHLIERVFPYRF
                     HHIEEFKKMGLKAEYFNPIVDNPASYYEFNPESNRPEYFHGVKIFGPAKLQPADFMVN
                     DLRTGASLTLAALTAEGKSSIEGVENIERGYEKLADRLCYLGANIEYIKT"
     gene            3151..4185
                     /locus_tag="PK131_01315"
     CDS             3151..4185
                     /locus_tag="PK131_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44803.1"
                     /translation="MTHLYLGLLLFSFLFTSVLIIPFINLLFKLKFQRRRQVTKDFLN
                     QHTPIFDKFHSQKSGTPVGGGVLIIISVCCLFFFLFHLLKFSQIFISTNYQINLEIFI
                     IFFTFISFGLIGLYDDVFKFFGFKKTGFFGLRMRNKLLLQSLLALFISWLIYAGLKIN
                     FIYIPFLGAVKLYWFFIPVATTIIVAFTNFFNITDGLDGLACGLLTIALFAFWALSNN
                     SLDTPISLFLALWIGALIAFLYFNVFPARIWLGDVGSLPFGATIAVIAILLGKIVPLI
                     IVGMPFIIEGLSSAGQIISKRYWHRKLLPAAPFHLTLQQIGWEEPKIVTRAWLAGIIL
                     AIFGLWLGLN"
BASE COUNT         1253 a          921 c          723 g         1442 t
ORIGIN      
        1 actcgtttcc cttggtcgga agcagaagcc tttgaactgc ttttagaata aaaaatctat
       61 gagtcaattt aatttaactg taactgatat tgccactgcc tacgaaaggg gattactacc
      121 tcaacgaaat aatcgatctt acttctatcg ggataccgct tgccgttcta atttagccaa
      181 cttctccctt tcctcagaaa atcgccgtat cttaaaaaaa acccaacaat tttccttccg
      241 taaaactcct ctaaaagact tcaagtttga tcttgaaatc caaaaacaaa tttaccactg
      301 gactaaaact ttaaaatgga attttccgat aagttcagta aaaaatgtct ttactaatca
      361 ttttttcaat tatctttatg attggactga taacaacaat cagtcggtag cttatgctat
      421 ttgctatttt tcgacctcaa ttgcccatat tgcttacgtt ttttataatc cccgctacaa
      481 tcacagcgat cttcctatcc gcttagttct ccaggtggta attgacagtc acgacttaaa
      541 tttaacttac tgttatcttg gccgcttttc aacaactaca ggctattata aacgggctat
      601 gcccggattt gaatattttt cccaaggtaa gtggaaaaaa tataattaaa atgaaaattc
      661 atattctagg gatagcaggg tcgatgacta cccccatagc tctttattta aaacaacaag
      721 gtcatactat tagcggttct gatcaggata aaatttatcc accagcaagt gaccttttaa
      781 aaaacattcc ctttaatcag ccattacctt ccaagattga cctttgtctt gttggtggtt
      841 cttataataa attccaaagg acaagacaag aattcgcttt tattcataaa catcactatc
      901 cctatatctc cactactcat ttcctcgcca atcagctaaa ttccttaaat cctatcctaa
      961 ttgcaggaac tttcggtaaa acaagtatta gtgctcttct ggcctggaat ttatctcagg
     1021 ctggtttcca acctaattat ttctttggcg gcattgctct taatcaattt ccctctttag
     1081 ctatcaccgg ttcagattgg tcggttatcg aagctgacga atcaataaac ggtcttgaca
     1141 cccaagctaa atttttatct tatccaatta aatatctcat cctcacttcg gccgcctggg
     1201 agcatcgaga ctgttaccga acggctaaag ataattttga agctttccgt cagttggtcc
     1261 aaagattacc ggctgatggc ctattaatcg cgaataaata tggaaaaaat atcagtgatt
     1321 taattaaatt ctcaccagcg ccggtaatct tttactcagg ttccaatgaa gaagcagtag
     1381 ccactttttg tcaacaattt aaaattaaat ttgacccaaa tttcccgggt ttaaagcgcc
     1441 gcctagagtt aattgatcgc cgaaataata tcttaatttt tgatgatttt gcccaatcac
     1501 ctgatagaat tagctacact ctcaatcttc ttcgaaatcg ttatcccaaa tataatctaa
     1561 aggttttatt tgaaccccat gccagttttc tccgctactc tattgccaac ttaggtcaag
     1621 cattggaatc ggctcaggag gttattttgg gagccattac ctatactcac gacaaaaacc
     1681 ataggattac tcttaaggat tatcaaaaag aaattggtcc caaactaatt tacctgcctc
     1741 ttaaaacaga tataattaac tactaccgac agactttaaa gccgggtgat cttcttattc
     1801 gctttgcttc agccggagaa aacttatgtc tcaatatata attaacggcg gtaaagctct
     1861 aaagggtgag gtttctatcc gaggagctaa aaacgcctca tttaaacaaa ttattgccac
     1921 tttactaacc gacaaaactt gtcaactgga taatgtcccg gtaattgctg atgttaaaat
     1981 cactgaaagt attgccaaac aattaggcac ccaaattaag ccggtggggg agcatagcat
     2041 agaattaacc acttccacta tcaccagcac cacagttcct tttggcactg gtcaaaaatc
     2101 ccgaagttca ttcctctttg cggccccgct tttagctaga aaaggtcaag caactttccc
     2161 cttacccggt ggtgataagc ttggcgatcg gccattagac cgcttttttg attgtctaaa
     2221 aagaatgaat gttaaaatcc acgaaaacaa tggcattatt acattaacaa ccactaatct
     2281 caaagctact cagtaccgat ttaataaacc cagtcacact gttaccgaag cagttcttct
     2341 tttggcaact cttaccccgg gaacttccat atttcaaaat tgtgctcggg aaccagaaat
     2401 tgacgattta attgaatttt tgaaggatat gggggcaaaa atcaatcgtg attctaaata
     2461 tcctgatcaa attcaaatta ctggcgtttc gaagttaact ggtgccaagc atcaagtaat
     2521 cgccgataga aatgaggccg ttactttcgc ttgtgctgct ttagccacta aaggctctgt
     2581 taatattctt cggatcaatc cagctattat tgaaacattt cttcaaacgg tggaacaaat
     2641 gggggctagg gttgaccgag gtaaagaaga attaattatc agttgggtac aaccattaaa
     2701 agctgttgat attgaaaccg ggccggaacc aatgtttatg accgattggc agccggtttt
     2761 taccgtggtt cttagccaag caataggttg cagtcatcta attgaacgtg tttttcctta
     2821 tcgtttccat catattgaag agttcaaaaa aatgggtctg aaggctgaat attttaatcc
     2881 aattgttgat aacccggcta gctattacga attcaatccc gaaagcaatc gaccagaata
     2941 ttttcatggg gttaaaatct tcggcccggc taaactccag ccggctgact ttatggttaa
     3001 tgacttacgc accggtgcct ctttaactct agctgcttta acagctgaag gtaaatcatc
     3061 aattgaagga gtagaaaata ttgaaagggg ctacgaaaaa ttagccgatc gcttgtgtta
     3121 tcttggagct aatatagaat atattaaaac atgactcatc tttatcttgg tttacttctt
     3181 ttttctttct tatttacaag cgttttaatt atccctttta ttaatctttt atttaaatta
     3241 aaattccagc ggcgtcgcca ggtaactaaa gacttcctta atcaacacac tcctattttc
     3301 gacaagttcc attcacaaaa atcaggtact ccagtcggcg gcggcgtttt aatcattatc
     3361 tctgtctgtt gtttattttt ctttcttttc caccttctta aattcagtca aatttttatt
     3421 tccaccaatt atcaaatcaa tctggagatt tttattattt ttttcacctt tatcagcttc
     3481 ggactaatcg gcctttatga tgatgttttc aaattttttg gtttcaaaaa aacaggattt
     3541 tttggtctaa gaatgaggaa taaactctta cttcaatcgc tcctggctct ttttatttcc
     3601 tggctgattt acgccggcct taaaatcaac ttcatttata ttccctttct gggtgcagtt
     3661 aaactctatt ggtttttcat tccagtggcc actactatca tcgttgcctt tactaatttt
     3721 tttaacatta ccgacggatt agacggacta gcctgcggtt tactgactat cgctttattt
     3781 gccttttggg ctctctcgaa taattctctt gataccccga tttctctttt tttagctctt
     3841 tggattgggg ctttaattgc ctttctttac tttaatgtgt ttccggctcg aatttggtta
     3901 ggggacgtcg gttctttacc ttttggcgcc actattgctg taattgctat tcttttagga
     3961 aaaatcgttc cattaattat cgtcggtatg ccttttatca ttgaaggctt gagtagtgcc
     4021 ggccaaatta tctctaaaag atattggcat cgcaaacttt taccggcagc cccttttcat
     4081 ctcactcttc aacaaattgg ttgggaagag cctaaaattg ttacccgagc ttggttagcc
     4141 ggcattattt tagctatttt tggtttatgg cttggactaa attaattcct caatacttag
     4201 gtattcctta tcgacgtaat tctctttcaa cctatacccc tgatgatgtt ttagctggta
     4261 agggttcaat taaagccatt aatcagctaa cccggaggtt agctaaaaaa gaaaaagtcg
     4321 atttattccg actttctaa
//
LOCUS       DAOOIE010000029         4245 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_16277_length_4245_cov_2.771122, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000029 DAOOIE010000000
VERSION     DAOOIE010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4245
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16277_length_4245_cov_2.771122"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..267)
                     /locus_tag="PK131_01320"
                     /pseudo
     CDS             complement(1..267)
                     /locus_tag="PK131_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197853.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            341..490
                     /locus_tag="PK131_01325"
     CDS             341..490
                     /locus_tag="PK131_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44804.1"
                     /translation="MDKIDTDQIESTFQVNGLKNITRPDKVQSTSPIHQGFFVTSSLK
                     NQNER"
     gene            480..1694
                     /locus_tag="PK131_01330"
     CDS             480..1694
                     /locus_tag="PK131_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HPD44805.1"
                     /translation="MKDEINAFITLIDHPSPGIPIAIKDNICTKGIRTTAGSKVLADF
                     IPSYSATLVERLENAGFSIIGKTNLDAFAHGSSTENSDFGVTLNPYDHQRLPGGSSGG
                     SAAAVGAGLVDFAIGTETAGSIRQPAAWCGVVGFKPTYGYVSRYGVIAMASSMDCPGV
                     IANNINNVIKVFEVISGHDSKDATSLPLPPFRFKQDRLIDGLKIGIPKSYLKITAPEV
                     LEKFNQVGDIFRKLGHEVIEIEMLDPAIAIAVYTILQSSEVSSNLARFDGIRYGQDRR
                     YFNPENQRRILLGTYALSADSYEQYYIKALKVRTLIINDFNKHFKNIDVILAPTTPTT
                     ALPLGSSLDDPLFGEYADQLVEPSTLAGLPGITVPCSLIDGLPVGFQIIGPRLSENLI
                     LNLALQYESIKN"
     gene            1708..3123
                     /gene="gatB"
                     /locus_tag="PK131_01335"
     CDS             1708..3123
                     /gene="gatB"
                     /locus_tag="PK131_01335"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HPD44806.1"
                     /translation="MKIIPIIGLEVHLELKTKSKMFCSCSAAHFQAEPNSQTCPVCLG
                     FPGTLPVPNQTAIDWCLKLGLALNCRINQYSYFERKNYFYPDLPKGYQISQLHQPFAV
                     GGFLEIEGRKIRLNRVHLEEDSGRCLRQNGQIQLDFNRAGVPLIEIVSEPDLTSLEIV
                     KKFLSRIQQIARYLDVADADIEKGSMRCEPTVNLKIEEDDHIFYTPLVEIKNIAGLGE
                     VAAALDFEINRQQKEFTETRQEKDKPPAGGKTTRGWNAGQKRTFLQREKEEVADYRYF
                     PEPDIPPFEFSEEYIEKIRQEIPELPDQKIKNYLNWGLNQTEAEIMSYDRNFALCFEK
                     AVGNQTKLAKSIANLFLGPLKFNLDLEKINPIFFHDLIKALNKNEISATVAKQIILES
                     YQTGKNPLEIAKERNLFQNSDIEDLKTIAQKVLADNPQAVADYRKNPNSIGFLIGQLM
                     KVSAGRANPQLARQVLEELLK"
     gene            3182..>4245
                     /locus_tag="PK131_01340"
     CDS             3182..>4245
                     /locus_tag="PK131_01340"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPD44807.1"
                     /translation="MEAYFKNLNDKQRQAVEAIEGPVLVIAGAGTGKTHTIAARIANI
                     LTKTDINPENILCLTFTDNAVTNMRERLKEVIGVTSYKVRLHTFHSFSNEVIRANLDK
                     FIFAKDINLLSDGERLEIILSLIDQLDDGAALKPWGDHYYYRSQIESLIQSLKREDVS
                     PEKLLDLIRDEKEFVEKTGDLFLKLKESRKEVLDIFAKIYEISLAYPPLAEFLNYQKI
                     FFDQGGYDKGLAKNPLINFKNALWKTFTSWRQNIDKQLNLQQIYAEYQVELKRRGRYD
                     YDDMILMVLNKFKQDVDLLADYQEQYQYILVDEYQDTNTAQNELLFLLASYDNQPNLF
                     VVGDDDQSIFRFQGANLENI"
BASE COUNT         1304 a          743 c          844 g         1354 t
ORIGIN      
        1 taatgcctta ttcaatctat cttcaaaaaa tatactaagc tgagagattg tcaaagccca
       61 attttgaatt ggcattgtcc atttttgttc ggcattgcaa atgcccatgt aaagcagctt
      121 taacaggctg ttttcattag caaaaccgcc cttagttttg gtcagtgccc taaactggcg
      181 atgaacagat tcaataacat tggtggtgta aattatccgt cttattgcct ctggatactt
      241 aaaatagtta gataaattat cccatttagc caatttaccc ataaataacc caaccgagat
      301 tattgatgat ttaaataaaa ttacggttca atttaacgtt ttggataaaa ttgataccga
      361 tcaaatagaa tctacttttc aagtaaacgg tttgaaaaat attacccggc cggataaagt
      421 tcaatcaact tcgcctattc accaaggatt ttttgttact tcttctttaa aaaatcagaa
      481 tgaaagatga gatcaatgct tttattactt tgatcgatca cccttctccg ggtattccca
      541 tagcgattaa ggataatatt tgtaccaagg gaataaggac taccgccggc tccaaagtat
      601 tagcggattt tattccttct tattcagcaa cccttgttga acggctggag aatgccggtt
      661 tttctattat tggtaagact aatctagatg cttttgctca tggttcctca acagagaatt
      721 ctgattttgg agtaaccctg aatccttacg atcatcaacg attacccggt ggttcttccg
      781 gtggttcagc cgccgctgtt ggggctggtt tagtcgattt tgccattgga acagaaacgg
      841 ccggttctat tcgccagcca gccgcttggt gcggggtggt tggttttaag ccaacctatg
      901 gttatgtttc tcgctatgga gtgattgcca tggcctctag tatggactgc cctggagtca
      961 tcgccaacaa tattaataat gttattaagg ttttcgaggt tatttccggc catgattcta
     1021 aagatgcgac ttcgctacca ttaccgccgt ttcggtttaa acaagaccga ttaattgatg
     1081 gattaaaaat tggtattcct aaatcatatt taaaaatcac tgccccagag gtcttagaga
     1141 aatttaacca agtcggcgat atttttagaa aattaggcca tgaagttatc gaaattgaga
     1201 tgttggatcc ggcgatagct attgctgtct ataccattct tcaatcgtca gaggtctctt
     1261 ctaatttggc tcgatttgat ggaattcgtt atggtcagga tcgccggtac tttaatccgg
     1321 aaaatcagcg tcggatcctc ttgggaactt atgccttatc agccgattct tacgaacagt
     1381 attatattaa agccttgaag gttaggacat taattattaa tgattttaat aagcatttta
     1441 aaaatattga tgttattctg gccccaacta ctccgacaac agccctgccg cttggctcgt
     1501 ccttagatga tcctcttttt ggtgaatatg ctgatcaatt agttgagcct tctactctgg
     1561 ctggacttcc gggaattacc gtaccatgca gtttaattga cggattgcca gtaggtttcc
     1621 aaattatcgg tcctcgatta tctgaaaatt taattcttaa tctagctctt caatatgaat
     1681 caattaaaaa ttaagaatta aaaattaatg aaaattattc ctatcattgg tcttgaagtt
     1741 catcttgagc tgaaaacaaa gtcaaaaatg ttttgttctt gctcagcagc ccattttcaa
     1801 gctgaaccta atagccaaac ttgtcctgtc tgcttgggtt ttccgggaac tttgccagtt
     1861 cccaatcaga cagcaattga ttggtgtctc aaattaggac tagcccttaa ttgtcgaatt
     1921 aatcaatatt cttattttga acgaaagaat tatttttatc cggatttacc caagggttat
     1981 caaatcagtc aactgcatca accctttgct gtcgggggat ttttagaaat agaaggccgg
     2041 aaaattcgtt taaaccgggt tcacttagag gaagatagtg gtcgttgtct ccgtcaaaat
     2101 gggcaaattc aattagattt caatcgagcc ggggtccccc taattgaaat tgtttctgaa
     2161 ccggatttaa cttcgctaga aattgttaaa aaatttttaa gcagaattca acaaattgct
     2221 cgctaccttg atgttgccga tgccgatatt gagaaaggat caatgcggtg tgagccgaca
     2281 gttaatttaa aaattgagga agatgatcat attttttata ccccattggt ggaaattaaa
     2341 aatattgctg gactgggaga ggtagcagcg gctcttgatt ttgagattaa tcgccagcaa
     2401 aaagagttta ctgaaacccg ccaagaaaaa gataagccac cggccggcgg caagaccact
     2461 cggggttgga atgctggtca aaaaagaacc tttttacaaa gggaaaaaga agaagtggct
     2521 gattatcgtt attttcccga acctgatatt cctccgtttg agtttagcga ggaatatatt
     2581 gaaaaaatta gacaggaaat tcctgaacta cctgatcaaa aaataaaaaa ttatctgaat
     2641 tggggcttaa atcaaactga agcggaaatt atgagttatg accgaaattt cgccctttgt
     2701 tttgagaagg cagtaggcaa tcagacaaaa ttagccaaat ctatagccaa tctatttttg
     2761 ggaccgctaa aatttaattt agatctcgag aaaattaatc cgattttttt tcacgattta
     2821 attaaggcct taaataaaaa tgagatttcg gccacggtgg ccaaacaaat tattttagaa
     2881 agttatcaaa caggcaaaaa tcctttagaa atagctaaag aaagaaattt atttcaaaat
     2941 tcagatatcg aggatttaaa aacaattgcc caaaaagttt tggccgataa tcctcaggca
     3001 gttgctgatt atcgtaagaa tcccaattct atcggttttt taattggtca gttaatgaag
     3061 gtttctgctg gtcgggccaa tccccagtta gcccggcagg ttttagagga attattgaaa
     3121 taattgacca tttctgacgg gtaacattat ttaattacag catggtttgt taaaatacat
     3181 tgtggaagcg tatttcaaaa atctcaatga taaacagcgt caggcggttg aagctattga
     3241 agggccagtt ctggtaattg ccggggccgg aacgggaaaa acacatacaa ttgccgctag
     3301 aatagctaat attttaacca aaactgatat taatcctgag aatattctct gtttaacttt
     3361 taccgataat gccgtaacta atatgcggga aagactgaaa gaggtgattg gggtaaccag
     3421 ttacaaagtc cggcttcata cttttcactc ttttagcaat gaagttatca gggctaatct
     3481 tgacaagttt atttttgcca aagatatcaa tttattaagc gatggtgaac gcttggaaat
     3541 tattttaagt ttgattgatc aattggacga cggcgccgct ttgaaacctt ggggggacca
     3601 ttattattat cgcagtcaaa ttgagtcgct aatccaaagt cttaaaaggg aagatgtttc
     3661 tcccgaaaaa ttgcttgatc tgataaggga tgaaaaagaa tttgttgaga aaaccggtga
     3721 tttatttctc aagttgaaag aaagtcggaa ggaggttttg gatatttttg ctaaaattta
     3781 tgaaatttcc ttggcttatc cgccgctggc agagttttta aattatcaaa agattttttt
     3841 tgatcaaggt ggctacgata aaggtctggc taaaaatcct ttgattaatt ttaaaaatgc
     3901 tctttggaaa acttttacca gctggcggca aaatattgat aagcagttaa atttgcagca
     3961 aatttatgct gaatatcagg tggaattaaa aaggcgcggt cgttatgact atgacgatat
     4021 gattttaatg gttctgaata agtttaaaca agatgttgat ttgttagcag attatcagga
     4081 acaataccaa tatattttgg ttgatgaata tcaggatact aatacggctc agaatgaatt
     4141 gctttttctc ttagccagct atgataatca gcctaattta ttcgtagttg gtgatgatga
     4201 tcagtcaatt tttcgttttc aaggagctaa tttggaaaat attta
//
LOCUS       DAOOIE010000030         4225 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_16378_length_4225_cov_3.531894, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000030 DAOOIE010000000
VERSION     DAOOIE010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16378_length_4225_cov_3.531894"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1566
                     /gene="murJ"
                     /locus_tag="PK131_01345"
     CDS             <1..1566
                     /gene="murJ"
                     /locus_tag="PK131_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HPD44808.1"
                     /translation="SIMAASLVLAATFVISAILGFLRTRILYDHFFQTSILELDAFNA
                     AFRLPDLIFKLLVTGAISASFIPVYTSYLYKNKALADKVASSTINLLFLAFMVISLIV
                     FILANPFSRLIARGFSPYQISVMASLTRILLLAQIFFLFSNFLTSILQINQFFLVPAL
                     SPIVYNLVIISSIYLLGPKWGIYGVAWGTVAAACLHLLIQIPTLRRQGFHYQLIFNYH
                     LTGVKEVIHLMIPQAFSIGVSEIESTVILSFASLLTAGSVSLLNLALQLMYLPSRILS
                     TTVGQASLPILSRKVAEKNFGRFREIVFKTVIQSLFLALPIAVIVLVLRLDLVRLIFG
                     SKKFPWEATKVTAATLGFLTPAIVCQALIQIIIRAFYALHNTKIPLISAIISLIVCIV
                     SSFIFINSTSLGIAGLALGISLGDISQSLSLVVCFALKVKGFDWKEILNRFLLIIFSS
                     LIGGLCCWGSMRLLDGYLFDTSRVIGVACVFIFSSLIGLASYFLTAFGLHLEEVADYK
                     QYLLRLKHFIYRR"
     gene            1600..2148
                     /locus_tag="PK131_01350"
     CDS             1600..2148
                     /locus_tag="PK131_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44809.1"
                     /translation="MASKKKEISLIRSADRPTSLSEKVIYWVSNVLRYIMVFTELIII
                     IAFLSRFKLDQEHTDLSESLRNKRYILETTSNFEKEYSQLQQKLKLIKTLYDSQPDYY
                     AKLSSIIQNTPADIVYNTLSVNNKDNKISFNLSVYAYQEEAVIDFINQLSLNPQIKSI
                     NISSIEKKARENKYNISLNLGL"
     gene            2145..2768
                     /locus_tag="PK131_01355"
     CDS             2145..2768
                     /locus_tag="PK131_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44810.1"
                     /translation="MSNSFLPRLKTEKLLEFVAKEGENNQSRRFIEIGFTLIVIAGFA
                     FFALKPTFATISDLMGKIKSKRILVTEMSKKIGNIIKAQDNFANIQNEYPIIESCLPS
                     RPAVFQATTQVRKSGEKFSVSFDRITFTFEEGNSYHLNVNTKGDFIAFLQTLGQLNRT
                     RRPFTINSFSFSLPKMQSAAATSSADLVNFNLNTEYYYSEKTNESKQ"
     gene            2752..2964
                     /locus_tag="PK131_01360"
     CDS             2752..2964
                     /locus_tag="PK131_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44811.1"
                     /translation="MKASSKISKYFLTVAVLILIIVCLAIYSISLDKYLKPINEAKEN
                     ELIKPFDPQLDMDTIKEIMSREDLSD"
     gene            2964..>4225
                     /locus_tag="PK131_01365"
     CDS             2964..>4225
                     /locus_tag="PK131_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44812.1"
                     /translation="MKKEKTIPTLVGLAILAVSLIVGLLLTNNTRIFSPRASSDCSPK
                     DLKITNISHYSATVSLTTNTECSVNLSLNGRIMADTLIQPDKTHYFELTNLNPNTDYT
                     FDVISGGKSYKSDNYKIKTAVKPQSTTPTSRLAWGKVFYQDLRPAANSIVYLSIPSAA
                     PLSGLVTNNGYWYISLATSFNKNLTDWFKLVDGVDETIEVLAPDLSRTIVSGNTSKNN
                     PVPDIIIGQNVGLGGSFEQVTQVPLQKSLSVISPKEAEIITVRRPDIFGNAASHAIVV
                     VNLGDIKDSVQAGSDGSWHWYPQVDLPPGSNQLKVVAGEETVVRNFIISVDSGSLAFT
                     ASESARLISPTSTPIPSTITSVFTPTPTQIATPTVTPSTSPRPTSTPAVRTAKPATDG
                     GVPATGVILPTIGSLLGALTLIGIALVLR"
BASE COUNT         1186 a          752 c          753 g         1534 t
ORIGIN      
        1 tcaattatgg ccgccagtct tgttttggcg gcaacctttg ttatttccgc tattttgggt
       61 tttttacgaa ccagaattct ttacgaccac ttttttcaaa ctagtatttt ggaattagat
      121 gcctttaatg ctgcctttcg tttaccggat ttaattttta aacttttggt taccggagca
      181 atttctgcct catttatccc tgtttataca agttatcttt ataaaaataa ggctttggct
      241 gataaagttg cttccagtac cattaatctt ctgtttttgg cctttatggt tatttctttg
      301 atcgttttta ttttagccaa tccttttagt cgattaattg ctcggggttt ttctccttac
      361 caaatctcgg ttatggcttc attaacccgg attttacttt tagcccaaat attttttctt
      421 ttcagtaatt ttttaactag tattttacag attaatcagt tttttttagt accggctcta
      481 agcccaattg tctataactt agtgattatt tcgagtattt atcttttagg gccgaaatgg
      541 ggaatttatg gtgttgcttg ggggacagta gcagctgcct gtttacattt attaattcaa
      601 atccctacct tgcggcggca gggttttcat tatcaattga tttttaatta ccatttgaca
      661 ggggtaaaag aggtaattca cttaatgatt cctcaagctt tttccattgg cgtttccgaa
      721 attgaaagta cggttatttt gagttttgct tctttattga cagccggatc tgtttctctt
      781 ttaaatttgg cattacagct tatgtattta ccctcacgaa ttctttcaac tactgtcggt
      841 caagcttcct tgccgatttt atcgagaaaa gtagccgaga aaaattttgg tcgtttcaga
      901 gaaatcgttt ttaagacagt gatacagagt ttatttttgg ctttaccgat agcagtaatt
      961 gttttggttt taagattaga tttagttcgg ctcatctttg gctctaaaaa atttccttgg
     1021 gaggcaacta aagtaacggc ggcaactctt ggatttttaa caccggctat tgtctgtcaa
     1081 gctcttattc aaatcattat tagagctttt tatgccctac ataatactaa aattccctta
     1141 atttccgcta ttatttcttt aatagtttgt attgtttcta gttttatttt tattaattca
     1201 acctcccttg gaattgccgg cttagctttg ggaatttctt tgggtgatat cagccaatcc
     1261 cttagtttgg tagtttgttt tgccctcaaa gtcaaaggtt ttgactggaa agagatttta
     1321 aatcgtttcc ttttaattat tttttcttcc ttgattggtg gcctttgctg ttggggttca
     1381 atgcgtttat tagatggtta tctttttgat accagtaggg tcattggagt agcctgtgtt
     1441 tttattttct cttcattaat tggtttagca agttattttt taactgcttt tggacttcac
     1501 ctggaagaag tcgctgatta taaacaatat ttactccgtt tgaaacattt tatttatcgg
     1561 cgatagttgt atatactgta tttaaagtaa ttaaaattta tggcttctaa aaaaaaggaa
     1621 atttctctga tacggtcggc tgatcggcca acatcgttat cagagaaggt gatctactgg
     1681 gtgagcaatg tgttgcgcta tattatggtg tttaccgagc taattattat tattgctttt
     1741 ctctctcgtt ttaagctcga tcaagaacat accgatttat ctgagagcct acggaataaa
     1801 agatacattt tggaaacaac gtctaatttt gaaaaagaat atagtcaact tcaacagaaa
     1861 ctaaagttaa ttaagactct ttatgacagt caaccggatt attacgcaaa actttcatct
     1921 ataattcaaa atactccagc tgatattgtt tacaatactt tgagtgttaa caataaagac
     1981 aacaaaatat cttttaattt gtcagtttat gcttaccagg aagaagctgt tatcgatttt
     2041 attaatcagt taagccttaa ccctcagatt aagtcaatta atatcagtag tatcgagaaa
     2101 aaagcccggg agaataaata taatattagt ttaaatttag gtctatgagc aatagttttt
     2161 tacctcgcct aaaaaccgaa aagctacttg aatttgtagc gaaagaaggg gaaaataatc
     2221 agtctcgccg ttttatcgaa atcggtttta ctttaatagt aattgctgga tttgccttct
     2281 ttgctttgaa accaaccttt gctactattt ccgatttgat gggtaaaatc aaatcaaaaa
     2341 ggattttggt aactgaaatg agtaaaaaaa ttggaaatat tattaaagct caagataatt
     2401 ttgccaatat tcagaacgaa tatccgatta ttgaatcgtg tcttccttcc cggccagccg
     2461 tttttcaggc aactacccaa gttcggaaat caggggaaaa attttcggtt agttttgaca
     2521 gaattacctt tacttttgaa gagggcaata gttaccatct caatgttaat actaaaggag
     2581 attttattgc ctttttacaa acccttggtc agttaaatcg aactcgtcgt ccttttacta
     2641 ttaatagttt ttctttttct cttcctaaaa tgcagtcggc agcggctact tcttcggcag
     2701 atctagttaa ttttaacctt aatactgagt attactactc agaaaaaacc aatgaaagca
     2761 agcagtaaga tatcgaaata ttttctaacg gttgcagttc ttattttaat tattgtctgt
     2821 ctggctattt atagcataag tttagataaa tatttaaaac caattaatga agctaaagaa
     2881 aatgaattga ttaaaccttt tgatcctcaa ttagatatgg acactattaa agaaattatg
     2941 agtcgagaag acttgtctga ttgatgaaaa aggaaaaaac tatacctaca cttgttggtt
     3001 tagctatttt ggctgtaagt ttaatagtcg ggttactttt aacgaataat acaaggatat
     3061 tctccccgcg ggccagtagt gattgttctc caaaagattt gaaaataact aatattagcc
     3121 actattcagc gactgtttcc ttaaccacta acacagaatg ttcggttaat ttaagcctca
     3181 atggcaggat tatggccgat actttaattc aaccggacaa gactcattat tttgaactga
     3241 ctaatcttaa tcccaatacc gattatactt ttgatgttat tagcggtgga aaatcgtata
     3301 aaagtgataa ttataaaatt aaaacagctg taaaacccca atcaactaca cctacttccc
     3361 ggttggcttg gggaaaagtt ttttatcagg atttaagacc agccgcgaac agtatagttt
     3421 atctttctat tccttcagca gctccgctct cgggtttagt aactaataat ggctattggt
     3481 atatttcttt agctactagt tttaataaga atttaactga ttggtttaaa ttggtagatg
     3541 gtgttgatga aactatagaa gtattagcgc cggatttatc ccggacaata gttagtggca
     3601 atacctctaa aaataaccct gtccccgata ttattattgg gcaaaatgtt ggtcttggcg
     3661 gtagttttga acaagttact caggtaccat tgcagaagtc gttgtctgtc attagcccta
     3721 aagaagccga aattattacc gttagacgtc ccgatatttt tggtaatgcc gccagtcacg
     3781 ccatagtggt ggttaatctt ggtgatatca aggattcagt ccaggcggga agtgatggca
     3841 gttggcattg gtatccccag gtcgatcttc ctccggggtc aaatcaatta aaggttgttg
     3901 caggtgagga aactgttgtt cgcaacttta ttatttctgt cgattctggc tcgttggctt
     3961 ttacggcttc agaatcagcc cgattgattt cgccaacttc tacacccatt ccatctacta
     4021 ttacctctgt ttttacccca acccccactc aaattgctac ccctaccgtt actccttcaa
     4081 cttcccctcg tcccacttct actccggctg ttagaactgc taaaccagca acagatggtg
     4141 gtgtaccggc aacaggtgtc attttaccaa cgattggtag tttattaggg gctttaactt
     4201 taattggaat tgctttagtt cttcg
//
LOCUS       DAOOIE010000031         4193 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_16527_length_4193_cov_3.214596, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000031 DAOOIE010000000
VERSION     DAOOIE010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16527_length_4193_cov_3.214596"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2059)
                     /locus_tag="PK131_01370"
     rRNA            complement(<1..2059)
                     /locus_tag="PK131_01370"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(2235..2308)
                     /locus_tag="PK131_01375"
     tRNA            complement(2235..2308)
                     /locus_tag="PK131_01375"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2272..2274),aa:Ala,seq:tgc)
     gene            complement(2470..3186)
                     /locus_tag="PK131_01380"
     CDS             complement(2470..3186)
                     /locus_tag="PK131_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44813.1"
                     /translation="MKEKEIDQWNKWRWYFLLKEVEIKPWPEVITPGKMEKLKQAGLN
                     FYYLPRVDLGTRDDLAKKGVEPYLTDLEKRYPDWKRHDLLDEKKRNNRFWWKNLEDLY
                     WRWVKDGQVDFPSLPGVWLAVSEARVYPGQTLIEINRTIKEQSRVILGEAGLSNEFEG
                     HLLEAVELNLLLNQLNHENQRKIILPELTATFYHNLENSLALVIGYPGQGEDVGKITP
                     LNPNLKADFIGARLAIVLTS"
     gene            3255..3328
                     /locus_tag="PK131_01385"
     tRNA            3255..3328
                     /locus_tag="PK131_01385"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3289..3291,aa:Ile,seq:gat)
     gene            complement(3333..>4193)
                     /locus_tag="PK131_01390"
     CDS             complement(3333..>4193)
                     /locus_tag="PK131_01390"
                     /inference="COORDINATES: protein motif:HMM:NF020032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPD44814.1"
                     /translation="ILVFTMSPSQSRFEIKRIKEEGRKIGIYIERWLYNELIFKNNQV
                     FLGKKEVDESNTLGVIFRAAGTISGKYVEGRNLLISYLSDKVKCLNAAGYLNWPRMGK
                     IPQLGVFLKNKVPVIPTKIFYRFDQVLAENFEFPIVVKDSLGYQGKGVERLNSLTELK
                     IFLKKLEEKDLGIYLWQKYLPVNWDIRVIVLGGKVLGAMKRTAVGEEFRSNFSLGGKV
                     EKWDLAAGDREIAEKVAKICYLDYAGIDIIHYNGKSYVLEVNRQCQFKGFEKATGINV
                     AKELIAFFLK"
BASE COUNT         1125 a         1067 c          669 g         1332 t
ORIGIN      
        1 ctagggcttc aatcacaggc tattccactc gaaagcggag gtcacccgtt catttaacct
       61 tctagcaccg gtcaggtttc agcccctata tctcccctta cgggttagca gggacctgtg
      121 tttttggtaa acagtcgccg tgtattcttt tgttgtatgc cataaggcgg aagggcatct
      181 cgcaaagctt acgcccagtt aatttgccaa gttccttaaa catctgtctt ccaatcgtct
      241 tagtctactc gacttaccca ccagtgttgg ttttggtacg aattttttat attctagttt
      301 cgacactttt cttggataaa tggggtcaat aaaattacct caattaagag atttttattc
      361 gcgctttgct taataacgaa agggcggatt taccaacccc ttctggctaa acgcttaaac
      421 gcccgatcac ccggacgctc tatcttccat ctatcgtcat gcctaaactt aaaacgaaca
      481 taaaaaagaa ccggaatatt aaccggtaat ccatccacga ctccgccact acaacggatt
      541 aatgttaggc tcgcctaacc ctcagtcgaa caacgtagct gaggaaacct tgggtatttc
      601 gacgtccggg attctcaccc ggatttcgct actcatgtct gcattctcac ttctgactgc
      661 tccagctccc cttacaggta aaccttcgcc gcaatcagaa cgctctccta ctaactttgt
      721 gctccgcact cagaattctt agtctttaat ttttagtttt taattaaaaa ttagaaattt
      781 gaattctcgg tgcgaagcac taagttcttc atcttcggta ttatgcttga gcctcgttac
      841 atttttggtg cagtttttca atccgccgat ggcggatcga tcagtgagct attacgcttt
      901 ctttaaagga tggctgcttc tgagccaacc tcctgactgt cttagaaaaa ccacctcctt
      961 ctccacttag cataaattta gagaccttaa atgaaagtct gggatctttc cctcttgccc
     1021 ggccagctta gcccggtcgg actgactgcc aagctaaaat tagcggtatt cggagtttgg
     1081 ttaaaaacct tcgctaaaag cgaaagctct catccagtag ctctaccccc gctaatcacc
     1141 acttgacgct atacctaaat atatttcgga gagagccagc tatctccggt ttcgattggc
     1201 atattacccc taatcaccag tcatccgatg tctttacagc gacaaacagt tcgggcctcc
     1261 ccccgacttt catcggagtt caccctgctg atgattagct caaccggttt cgggtcattt
     1321 tcaagcacct attcgcgctt ttcgcactcg ctttcgcttc gcctcctccg ccaagggcgg
     1381 attaaactcg atgcttaaaa atactcgcag actcattctt caataggcac gagatcatcc
     1441 gccaaatggc ggactctctc tgtttgttag ctgatggttt cagatctatt tcactcccct
     1501 atctagggtt tttttcacct ttccctcacg gtactagttc actatcggac cgctaaagta
     1561 tttagccttg gagattggtc tccccagatt cctacggtat tttacgtgga ccgcagtact
     1621 taggtatcca actaaaacag gatgaaaatt aaaatacagg actttcacct tctttggtag
     1681 cccattccat agctttcttc tttttcttcc tgttataagg tcggaccctg caacctccgc
     1741 ccgaaggcgg atttaggctc atccgttttc gctcaccgct actaacggaa tctcttacga
     1801 tttattttcc tccaggtact gagatgtttc agttccccgg cttaccccac actgcttcac
     1861 agtaaattta ctaattttca aaattgaaaa ttcactgtga aacagtgtgt aacactgctt
     1921 tcacagtgtc gggttacccc atttggatac tgtcggatta tcgcctattg acggctcccc
     1981 gacaaatttc gctgtctttt gcgtccttct tcggctttag cgaccaaggc atccaccatc
     2041 agctttatct aaaattccag taaaacttgc ccgccagtcg gcgggtgaca actcaattta
     2101 gttgccaaga aatttttaat tacttcgatt caacagattg ttaatgtaca aaaacaattt
     2161 gaggaaatta tcgaaataat tttctcattc ttgtctttaa ttaaaaatta attttgcttt
     2221 cggcaaaatc tgtgtggacc cagagggact tgaacccccg atttcctcgt tgcaaacgag
     2281 gcgctttaac caactgagcc atgggccccc agcattttta ttggttactt acataaaaaa
     2341 gccgaaggct ggatgccttc ggcgctatcc cttttcaggt caattctacc cctctttttt
     2401 tcttttattg gttttgatag gcttcagcat ctgcaagtgg tggtattcta cccaattttt
     2461 attctcccgt caactggtta aaacaatcgc cagacgggca ccgataaaat cggcttttaa
     2521 attaggatta agcggcgtaa ttttaccaac atcttctcct tgaccaggat aaccaataac
     2581 caaagccagc gaattctcca aattatgata aaaagttgct gttaattcag gcaaaataat
     2641 ttttctctga ttttcatgat taagctgatt cagcaacaaa tttaattcaa ctgcttctaa
     2701 caaatggcct tcaaattcat tcgataatcc agcttctccc aaaataactc ggctttgttc
     2761 tttaatagtc cgatttatct caattaaggt ctgaccagga taaacacgag cttcactcac
     2821 cgccagccaa acacccggta gagaaggaaa atcaacttgg ccatccttaa cccaccgcca
     2881 gtataaatct tctaagtttt tccaccagaa tcgattattt ctcttttttt catccaataa
     2941 atcatgtctt ttccaatcgg gatagcgttt ttctaaatca gttaaatatg gttccactcc
     3001 cttcttggcc aaatcatccc gggtacccaa atcaactcga ggcagatagt aaaaatttag
     3061 tccggcctgt tttaatttct ccatcttgcc tggagtaatt acttcaggcc acggtttaat
     3121 ttctacttcc tttaacaaaa aataccagcg ccatttattc cactggtcta tctctttttc
     3181 tttcattcat agtaattatc tcaattttat cgccaaattt caattatgat atactatttc
     3241 tgcaattaat taccgggtga ttagctcagt tggttagagc gccgtcctga taagacggag
     3301 gtcaatggtt cgaatccact atcacccact tattacttta agaaaaaggc aattaattcc
     3361 ttggccacat taatccccgt tgctttctca aaccccttaa actggcactg ccggttaacc
     3421 tccaaaacat aggatttccc attataatgg ataatatcaa taccagcata atctaaatag
     3481 caaattttag ccactttttc ggcaatttct ctatcaccag ccgccaaatc ccatttttct
     3541 actttacccc ctaaactaaa attcgaccta aattcttctc caacggccgt tcgtttcatt
     3601 gctcccaaaa cttttcctcc taaaacgata acccgaatat cccaattaac cggtaaatat
     3661 ttctgccata aatatatccc caagtctttt tcctctaatt tctttaaaaa aattttcaac
     3721 tccgtcaacg aatttaatcg ctccactcct tttccctgat atcccaagga atctttaact
     3781 actataggga actcaaaatt ttccgccaaa acctgatcga atcggtaaaa tattttagtt
     3841 ggaattactg gaactttatt tttcaaaaat acccccaatt gtggtatttt ccccattctt
     3901 ggccagttta aatagccagc ggcgttgaga catttaactt tgtctgatag ataagagatt
     3961 aataaatttc taccttcaac atatttacct gaaattgtcc ccgctgctcg aaaaattacc
     4021 cccaaagtat tactttcatc aacttctttc ttcccaagaa atacctggtt attcttaaaa
     4081 atcaactcat tatataacca ccgctcaata taaatgccta tctttcgtcc ttcttctttt
     4141 atcctcttta tctcaaaccg gctttgacta ggcgacatag taaagaccaa aat
//
LOCUS       DAOOIE010000032         4187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_16555_length_4187_cov_3.098258, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000032 DAOOIE010000000
VERSION     DAOOIE010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4187
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16555_length_4187_cov_3.098258"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="PK131_01395"
     CDS             complement(<1..530)
                     /locus_tag="PK131_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44815.1"
                     /translation="MKVLIVLLSALLLFGCGAKKPESLLPSSDENETEEMVGSNYVDK
                     LANEKIDFSQSFNLNQEFTVNFKTFEPEGEGVATFKARSIKEIPDVDGRTAGENNKLI
                     LVEISVRGNAKNKGLPSTFNQIGDYPSPQFVLVDKANNKSFVETNYFSTAYTASKNLF
                     ELDKITLDNEEWVKTA"
     gene            553..1062
                     /locus_tag="PK131_01400"
     CDS             553..1062
                     /locus_tag="PK131_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012460391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPD44816.1"
                     /translation="MLKYEILVVGQMKTNCYLVWDDCLKETVIIDPGDEADFISDEIE
                     KRQLKPIGILLTHLHSDHIGAAKDLQLIYQIPILNLPNKIFDFQIIKTPGHTTGSVCF
                     YSPTLQVVFTGDTLFKDLPARGENLDSIKKLLKLPPTTVVLPGHDEATTIGVEAGRYS
                     CQKDFTPLT"
     gene            complement(1134..1304)
                     /locus_tag="PK131_01405"
     CDS             complement(1134..1304)
                     /locus_tag="PK131_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44817.1"
                     /translation="MKAISQLAAELKLTPEQAGEIEQYFKELVVQLLENIKQDNLKNF
                     DETIEKIKTNTA"
     gene            1404..2867
                     /locus_tag="PK131_01410"
     CDS             1404..2867
                     /locus_tag="PK131_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008439845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HPD44818.1"
                     /translation="MKTKKFTILHSNDMHGDFLAEMKAGKKELIGGLSLLSGYINKVR
                     REEENVLYVISGDMLQGSLIDTEWKGCSTIEIMNYLAPDVVTVGNHEIDYGLPHLLFL
                     EKMANFPLINANLYIIKNNRRILQPYLIMKKAGFDILFIGIITEKIIDSLKQDKLIGT
                     FVGVEEASREVGKICNAYKNDDVDLTILLTHIGFESDIELAKLLKPEWGVDLIIGGHS
                     HTVLKKPAQINNILITQAGVGTNQIGRFDIVVDDDTNSIIEYKWQLIPIDNKLAKPDK
                     KLEEYIQSFQGEIDRKYNVILSKLSQTLTHPKREEETSLGNLIADALAENADCDVMLV
                     GSGSIRVKKLGPVVTLKDLLASFPYDDILKKFTITGAQLWNIFSHIMRTENRNGEGEC
                     YQVNRKVKAEYSDKQDKLLSLNIDGIPVDKTKFYTIGLQGYHFNNSANYLNITNEELL
                     ASGKSKVITTSAQQVLEEFLKNNQNISRKIEGRLVYK"
     gene            complement(2869..3066)
                     /locus_tag="PK131_01415"
     CDS             complement(2869..3066)
                     /locus_tag="PK131_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44819.1"
                     /translation="MKELFFWFLTPEKNRYLVIGLIMTILGVILIPFIIGIPIAGIGF
                     SIFAIGVLVSCGQKFSKFRKR"
     gene            complement(3072..>4187)
                     /locus_tag="PK131_01420"
     CDS             complement(3072..>4187)
                     /locus_tag="PK131_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44820.1"
                     /translation="DLQGCGDYFLRDNNMVNDCPGEDGWCVLTLAEKLVRRLGESLGG
                     RSGEPLIDSSTDDQTIILRNYTGLTFFGKNTWSKELESDPGFLSAAVTPGGDYVIVAK
                     DTARPVNSLAVFDKNGNDVTPKFDYKHVRFVEANDRGIFFATVVRNEMAYYQLGKYAA
                     EYKPPEANKPTDHISFGEIQIYNETEKKFKNVEETLTWENLSINTLYKIKDKIAVSTS
                     WGKLILSSGTVFAKNENGEPMLSWGQAEVEAVNPITMVVFKNKLPDAEKLYDAFSQYN
                     QGKLPENKYLLIHNLHTDYLVKTENQKVVIQIRKGEVEAISQGEKINVSEGNKIVIDE
                     NNKIFRSRLIPTWFYFLGIGVVLIILFLILPKHRVKT"
BASE COUNT         1398 a          795 c          677 g         1317 t
ORIGIN      
        1 aaagctgttt taacccattc ctcgttgtca agcgtaattt tatctaattc aaataaattc
       61 ttactggcag tataagccgt cgaaaaataa ttagtctcaa caaaagattt attattcgct
      121 ttatcaacca aaacaaattg gggtgaagga taatcaccga tttgattaaa agttgaaggc
      181 aaacctttat ttttggcgtt acctctgaca gaaatttcaa ccaaaatcaa tttattattt
      241 tctccggcgg ttcgaccatc aacatcggga atttctttaa tcgaccttgc tttaaaagta
      301 gccactcctt ctccttcagg ttcaaaggtt ttgaaattaa cagtaaactc ttggtttaaa
      361 ttaaagcttt gactgaaatc tattttttcg ttggccaatt tatcaacata attcgacccg
      421 accatttctt ccgtttcatt ttcatcagaa gatggtaaaa gtgattctgg ttttttggcc
      481 ccacaaccaa ataataagag ggctgataac aaaacaatta agactttcat ttaattatat
      541 tataatcaaa taatgttgaa atacgagatt ttagttgtcg gtcaaatgaa aactaactgc
      601 tatttagttt gggacgattg ccttaaagaa acggtaatta ttgatcccgg ggatgaagct
      661 gattttatta gtgacgaaat cgaaaaaaga caattaaaac caatcggcat tttattgacc
      721 catttacatt ctgaccatat cggtgccgcc aaagatctcc agttaattta tcagattcca
      781 attttaaatc tcccaaataa aatttttgat tttcagataa ttaaaacccc tggacacact
      841 acaggtagcg tgtgttttta tagtcctaca ctacaggtag tgtttactgg cgatacttta
      901 tttaaagacc tgccagccag aggagagaat ttagacagca tcaaaaaact gctgaaactg
      961 cctccaacca ctgttgttct ccccggccac gacgaagcaa ccaccattgg ggttgaagct
     1021 ggacgctatt catgccaaaa agacttcact cctcttacct aagagctcaa actagaaaga
     1081 aaggctaagt aaaacacata aggttgccga taaaccccag agcttcaata tatctaagca
     1141 gtattagttt tgattttttc gatcgtttcg tcaaaatttt tgagattatc ctgcttaata
     1201 ttttccaaca gctggactac taattcctta aaatactgtt caatttctcc tgcttgttca
     1261 ggagttaatt ttaattcagc cgccaactgg ctaatagctt tcatttagtt taaagtataa
     1321 tacattattt tcttaagtct atgtcgaaac ctaatagccg gccatacaga ccaaccagaa
     1381 agtgataaac tcagtttaga attatgaaaa ctaaaaaatt taccattctt cattctaatg
     1441 atatgcatgg ggatttttta gcggaaatga aagctggcaa gaaagaacta attggtggtt
     1501 tatctttact ttcgggttat attaataaag tcagacggga agaagaaaat gttttgtatg
     1561 ttatttctgg tgatatgctt caaggatctc taattgatac tgaatggaaa ggctgttcca
     1621 ctatagaaat tatgaactat ctagctcccg atgtggtaac tgttggtaat catgaaattg
     1681 attatggctt acctcatttg ttatttttag aaaaaatggc taattttcca ttaattaacg
     1741 caaatttata tattattaaa aataatcgtc gaattttaca gccatattta attatgaaaa
     1801 aagctggctt cgacattctt ttcatcggta ttatcactga aaaaatcatt gattctctta
     1861 aacaggacaa attaattgga actttcgtcg gagtcgaaga ggctagccgg gaagtgggaa
     1921 aaatctgtaa tgcctataaa aatgacgatg ttgacctaac cattctttta acccatattg
     1981 gctttgaatc tgacatagaa ctagccaaat tattaaaacc tgaatgggga gtggacttga
     2041 ttattggtgg tcactcccac actgttttaa aaaaacccgc tcaaattaat aacattctga
     2101 ttactcaagc aggggtaggc actaatcaaa ttggaagatt cgatattgtg gttgatgatg
     2161 acactaacag tattattgaa tataaatggc aacttattcc tattgacaat aaattggcca
     2221 aaccggataa aaaacttgaa gaatacattc agagttttca aggagaaatt gacagaaaat
     2281 ataatgtaat tttgtctaaa ctttcccaaa ctttaactca tcctaaaaga gaagaggaaa
     2341 cttctcttgg aaatttaatt gccgatgcct tagcagaaaa tgccgattgt gatgtaatgt
     2401 tagttggcag tggttcaatt cgagttaaaa aattgggtcc ggttgtaacc ttaaaggatc
     2461 tattggcctc ttttccctat gacgatattt taaaaaaatt cactatcacc ggagcccagt
     2521 tatggaacat ctttagtcat attatgcgaa cggaaaatag aaatggggaa ggtgaatgtt
     2581 atcaagtaaa tagaaaggtc aaagctgaat actccgataa acaagacaaa ttactctccc
     2641 taaatattga tgggatacca gttgataaaa ccaaatttta taccatcggt ctacagggtt
     2701 accactttaa caactcggcc aattatttaa atattaccaa tgaggaactt ttagcctcag
     2761 ggaaatcaaa agtgataact acatcagccc aacaggtctt ggaggaattt ttaaaaaata
     2821 atcaaaatat ttcaagaaaa atagaaggga gattagttta taaatagact atcttttcct
     2881 aaacttagaa aatttttgcc cacaagaaac caggactcca atagcaaaga tactgaaacc
     2941 aatcccagca atgggaatac caattataaa agggattaaa attactccca aaatagtcat
     3001 gattaaacct atgacaagat aacgattctt ttctggagtt aaaaaccaaa aaaacaactc
     3061 tttcattaaa cttaagtttt taccctgtgt ttaggtaaaa ttaaaaataa aataattaat
     3121 actacaccta ttcctaaaaa gtaaaaccag gtaggaatga ggcggctacg gaaaatttta
     3181 ttgttttcgt caattactat tttatttccc tcactgacat taattttttc tccttgggaa
     3241 atcgcttcta cttcaccctt acgaatctga ataacaactt tttgattttc agtttttacc
     3301 aaatagtcag tatgtaaatt atgaataagt aaatatttat tttccggcaa ttttccctga
     3361 ttatactgac taaaagcatc atacaatttt tccgcatccg gcagtttatt tttaaaaacc
     3421 accatcgtta taggattaac agcttctacc tccgcttgtc cccaggaaag catcggttca
     3481 ccattttcat ttttagcaaa aaccgttccc gaggaaagaa ttaattttcc ccaagaagtg
     3541 ctaacagcaa ttttgtcttt aattttataa agtgtattga tagaaagatt ttcccaagtc
     3601 aaggtttctt caacattttt aaattttttc tccgtctcat tataaatttg aatttcacca
     3661 aaggaaatgt gatccgtagg tttatttgct tccggcggct tatactcggc cgcatacttc
     3721 cctaattgat aataagccat ttcgtttctt actaccgtgg caaagaaaat tccccggtca
     3781 ttagcttcaa cgaaacgaac gtgtttataa tcaaatttag gagttacatc attaccgttt
     3841 ttatcaaaaa ctgccaaaga atttacgggc ctggccgtgt ccttcgccac aataacataa
     3901 tccccgcccg gcgtcaccgc cgcgcttaaa aaacccggat cgctttctaa ttctttagac
     3961 caagtgtttt tgccaaaaaa ggtcaggccg gtataattac ggagaataat cgtttgatca
     4021 tcggtgctgc tatcaataag cggctctccg cttctcccac ccagtgattc tcctaagcgt
     4081 cgcactaatt tctccgccag cgtcaaaaca caccagccat cttcacctgg acaatcgtta
     4141 accatattat tatcgcgcag aaaataatcc ccacaccctt ggagatc
//
LOCUS       DAOOIE010000033         4186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_16564_length_4186_cov_3.054708, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000033 DAOOIE010000000
VERSION     DAOOIE010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16564_length_4186_cov_3.054708"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PK131_01425"
     CDS             <1..496
                     /locus_tag="PK131_01425"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPD44821.1"
                     /translation="NLKAIINRCGCLAGPWQFGKVDQGVLTLWLAAHYFKKELAYIGF
                     GGRGKQVRDFLHIRDLFEILDIQINHFYQYSGEIYNIGGGIKNSTSLVNLTKICQKIT
                     GNKIKIKSIKKNRPNDVISYITDISKIKKQAHWSPQISLEKTIEDTYNWIKENESVLK
                     NILC"
     gene            490..1824
                     /locus_tag="PK131_01430"
     CDS             490..1824
                     /locus_tag="PK131_01430"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPD44822.1"
                     /translation="MLDILLVEPPSRRAFHYRPPIALLYLAGFLQKFKIKVKIWDSNK
                     KLDFQLIKKMNPKHIGLTCYTPEYEEVVQLAKEFKQLLPQTKIVVGGTHPTLYPQEFP
                     RKYFDKVIQGDGEEKLYHYLTKRRGRMAPTNFLPAYNLIDMSYYTHANPYAIRGCFLR
                     PAYLLASRGCPSECTFCVAKKLRQFTCFRTRPAEELIKEIKLLRRKYLIDSFYFIDDL
                     FTLNRNNVLKFCQLFQSAKLNLLWGCEAKVSTLDEELIKTMAAAGCIQIDFGVERGSN
                     QALKQCKKNITLTQIKNTFYLCHKYHIRTFANFLVNLPGETKKDLDDILKLAREIKPD
                     IVSFNIFTPYPGTEIYDSAKFKFTPEEYKLLYTGSNYLKKYPQKFKFARHNIDLSQWA
                     RINNKKFNRLGPIIKFHLSRRYLKTLLYSQNKLDYFLQLGNLLTEFYRQKFA"
     gene            complement(1793..3514)
                     /locus_tag="PK131_01435"
     CDS             complement(1793..3514)
                     /locus_tag="PK131_01435"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPD44823.1"
                     /translation="MSQKKYRRLRALEREREAIDDKRENLAVQEGFWEIIRKNWTFLL
                     IIGMGVVLIYLNALHGDFVSDDYASITQNPDVLNPRVMFSGFNTMRIGTYLLAVIFGI
                     KNPLGFHLNSLFLYLIFIILAFAFIYRMFGKKLAISTTLLFAFHPIHVEAVSWISGRI
                     YIILAIYILLGLMAFIYYLETGKYKFLGWVILFFILGFLTDKPRPFSLIFLMVLVVFY
                     FGWGKVRDKLSKFIPYIITFSIAFFILSIPYIRSRVAVVNSGYNFSESIFYNPFFQYP
                     TGIAKYLQLLWLPIDLTLYHTMYVFEPWLNWLILLLFLAIMWYWGKKDKRYWFALMFI
                     LLALAPSMAPVKVSWLVAERYMFLGSLGFCLFLALLLEAIDLRIKNLSYLILSLLLVF
                     YGIRVYLRNIDWQTNHNLWVRTVQYSWNSHNAWNNIGDDYDKLGQYDNAIKGFGQSYA
                     VKNNYADAYHNQANIFFKIKRYDLARQGYELALKFHPGLYQSYISLTQVALYQGDLNG
                     ALAYANKLIESQPNNPQAYYVMGVVLAQGGKLKEAEDYLNYALQLNPNFTPAVNTLKQ
                     ISAGKTQ"
     gene            complement(3517..>4186)
                     /locus_tag="PK131_01440"
     CDS             complement(3517..>4186)
                     /locus_tag="PK131_01440"
                     /inference="COORDINATES: protein motif:HMM:NF014042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase CofD family
                     protein"
                     /protein_id="HPD44824.1"
                     /translation="QFMTEKTGNLANVDRLFSELTGAELKGEVIPVSLEPADVCWQTE
                     SGKQYVGEHYFDEYRMSEDRVKKIWLEPMVEASEEAKQTLKMAEIIIVGPGSIYGSIL
                     VNFLPQGMITAYRSSQARKILIANLTSVANETAKLDAATYYKIFSNYLGKNSFDDIIE
                     PDFTKINPKLLTEVKRNYAYEHSYLYSSGSQVNDIVVVDRKNLRLRHDVKKLSLLFKR
                     ILRS"
BASE COUNT         1464 a          867 c          640 g         1215 t
ORIGIN      
        1 taatttaaaa gcgatcatta atcgctgtgg ttgtctggct ggtccctggc aattcggcaa
       61 agttgatcag ggcgtcctta ctctctggct ggccgctcat tactttaaaa aagagttagc
      121 ttatatcggc ttcggcggcc ggggaaaaca ggtccgtgat ttcctccaca ttcgagatct
      181 ttttgaaatt ttagatattc aaattaatca tttttaccaa tattcgggag aaatttataa
      241 tattggcggg ggaataaaaa atagcacttc tctggtaaac cttaccaaaa tttgccaaaa
      301 aatcactggt aacaaaataa agattaaatc catcaaaaaa aatcgtccca atgacgttat
      361 ttcctatatt accgacattt ctaaaattaa aaaacaggcc cattggtccc cacaaatcag
      421 tctggaaaaa acaattgagg atacttacaa ttggatcaaa gaaaatgaat cagttttaaa
      481 aaacattcta tgttagacat tcttctcgtt gaaccgccca gccgccgagc ttttcattat
      541 cgcccgccaa ttgccctgct ttatttagcc ggattcttgc aaaaatttaa aattaaagtc
      601 aaaatttggg attcaaataa aaaactcgat tttcaactaa ttaaaaaaat gaatcccaaa
      661 catataggct taacttgcta caccccggaa tacgaagaag tggttcaatt ggctaaagaa
      721 tttaaacaac ttttacctca aaccaaaatt gttgtcggcg gaactcaccc caccttatat
      781 ccccaagaat ttccccgaaa atattttgac aaggttatcc aaggcgatgg cgaagaaaaa
      841 ctctaccatt acttaactaa acgaagaggc agaatggccc cgacaaattt tctgccggct
      901 tataatctga ttgacatgtc ctactacacc cacgctaacc cttatgctat tcgaggctgc
      961 ttccttcgcc cagcttatct tttagccagc cgtggctgtc cttcagaatg tactttttgt
     1021 gtagccaaaa aattacgtca attcacctgt tttcgaactc gtcctgccga ggaattaatt
     1081 aaggagatta aattactcag acgtaaatac ttaatcgaca gtttttattt cattgacgat
     1141 cttttcactc ttaacagaaa taatgtttta aagttttgtc aattatttca gtcggccaaa
     1201 ttaaatcttc tctggggttg tgaagctaaa gtttcaactt tagatgagga attaatcaaa
     1261 acaatggcgg cagcgggttg tattcagatt gattttggag ttgaacgtgg ttcaaatcaa
     1321 gccttaaaac aatgtaaaaa aaatattact ttaactcaaa tcaaaaatac tttttatctc
     1381 tgtcataaat accatattag aaccttcgct aattttttag ttaatcttcc cggggaaact
     1441 aaaaaggatt tagatgacat tcttaaactg gctcgggaaa ttaaaccaga cattgtttct
     1501 tttaatattt ttactcctta tcctggcaca gaaatctacg actcggctaa attcaaattc
     1561 acccccgaag aatacaaact tctctatact ggttcgaatt accttaaaaa atatccccaa
     1621 aaatttaaat ttgcccggca taatattgac cttagtcaat gggctaggat caataataaa
     1681 aaattcaacc ggttggggcc aataatcaaa tttcatctca gccgccgcta tctcaaaacc
     1741 cttctttatt cccaaaacaa actagattat ttcctccaat taggcaattt gctcactgag
     1801 ttttaccggc agaaatttgc ttaagagtat taactgctgg agtaaaattt ggatttaact
     1861 ggagagcata attcaaatag tcttcggctt cttttaattt tcccccctgg gccaaaacta
     1921 ctcccatcac ataataggcc tggggattat tgggctgaga ctcgatcagt ttattagcat
     1981 aagccaaagc accattcaaa tccccctgat acaaagccac ttgagtgaga gaaatatatg
     2041 attggtataa accggggtga aatttcaagg ccagctcgta tccttgccga gctaaatcat
     2101 atcttttaat tttaaaaaaa atgttggcct gattatggta agcatcggca taattatttt
     2161 taactgcata tgactggcca aaacctttaa tagcattgtc atactggcct aacttatcat
     2221 aatcgtcgcc aatattattc caagcattat gactattcca agaatattga accgttctta
     2281 cccacaaatt atgattggtt tgccaatcaa tatttcgcaa ataaactctg ataccataaa
     2341 agactaacaa tagagacagg ataagataac ttaaattttt aatccttaaa tcaatcgcct
     2401 caagtaataa agctaagaaa aggcaaaatc ccagtgatcc taaaaacata tacctttcgg
     2461 ctaccaacca gctgacctta accggtgcca ttgacggagc taaagccagt aaaatgaaca
     2521 ttaaagcaaa ccaatatctt ttatccttct tgccccaata ccacatgatt gccaaaaaca
     2581 acagtaaaat cagccaattc aaccaaggtt cgaaaacata cattgtatga tacaaagtca
     2641 aatcaatcgg caaccaaagc aactgcaaat acttggctat accggtaggg tactgaaaga
     2701 acgggttata aaaaatactc tccgaaaaat tataacccga attgactacg gcaacccgag
     2761 agcgaatata aggaattgat aaaataaaaa acgcaatact gaaagtaata atatagggga
     2821 tgaatttact taatttgtcc ctaactttcc cccagccaaa ataaaaaacc actaaaacca
     2881 tcaagaatat taaagaaaaa ggccgaggct tatcggtcaa aaaacccaaa ataaaaaata
     2941 aaattaccca gcccaaaaat ttatattttc ctgtttccaa ataataaata aaagccatta
     3001 aaccaagtaa gatataaatg gccaaaataa tataaatcct acccgaaatc cacgaaaccg
     3061 cctcaacatg aattgggtga aaagcaaaaa gaagagtagt actgatagcc aacttttttc
     3121 caaacattcg gtaaataaaa gcgaaggcca aaataataaa tatcaaatat aaaaacaaac
     3181 tattaaggtg gaaacctaag ggatttttaa ttccaaaaat aactgctaaa aggtaagtgc
     3241 caattcgcat cgtgttaaaa ccagaaaaca ttacccgggg attaagaaca tccggatttt
     3301 gggtaattga ggcataatca tcagaaacaa aatctccatg aagagcattt agataaatca
     3361 aaactacccc catcccgatt atcaacaaaa atgtccaatt ttttctgata atctcccaaa
     3421 aaccttcttg aaccgctagg ttttcccgct tgtcatcaat tgcttccctt tctctttcta
     3481 aagcccttaa tcgacggtat tttttttgag acataattaa ctccttaaaa ttcgcttaaa
     3541 aagaagcgaa agctttttaa catcatgtct gagccgtaaa ttctttctat caaccaccac
     3601 gatatcatta acttgagaac cagaggaata aagatacgaa tgttcatagg cataattacg
     3661 cttaacttcc gttaatagtt ttggattaat tttagtgaaa tcaggttcaa taatgtcatc
     3721 aaaactattt tttcctaaat aattcgagaa gatcttataa taagtggcgg catctaattt
     3781 agccgtttcg ttagctactg aagttaaatt agcaattaaa atttttctgg cctggctgga
     3841 acgataggca gtaatcattc cttggggtaa aaaatttact aagattgaac cgtaaatact
     3901 tccaggacca acaataatga tctcggccat ttttagagtt tgtttagctt cttcactagc
     3961 ctctaccatt ggttccagcc aaattttttt aactctgtct tccgacatcc gatattcatc
     4021 aaaataatgc tcaccaacat attgcttgcc gctctccgtc tgccagcaaa catcggctgg
     4081 ttccaaagaa acaggaatta cttcaccttt taattcagct ccagttaatt cggaaaataa
     4141 ccggtcaaca ttagccaaat tacccgtttt ctccgtcata aactgg
//
LOCUS       DAOOIE010000034         4167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_16667_length_4167_cov_2.900535, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000034 DAOOIE010000000
VERSION     DAOOIE010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16667_length_4167_cov_2.900535"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            834..1355
                     /locus_tag="PK131_01445"
     CDS             834..1355
                     /locus_tag="PK131_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44825.1"
                     /translation="MLVIKNHPEVAVKTGTTNDMRDNWTIGYTPDYVVLTWVGNNNNA
                     RMGGLVSGVTGAAPIWHEITTQLLKDKEIKKPTKPAGIVGQSVCNLTGGAIPPEGCES
                     HFEYFKKEFLPPIIKPSRQNVLIDKDSGRIVKPEDHKPNTQTEEHLVFPDATGELICL
                     DCLIVEPTPTPLN"
     gene            complement(1347..1907)
                     /locus_tag="PK131_01450"
     CDS             complement(1347..1907)
                     /locus_tag="PK131_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44826.1"
                     /translation="MLPKTIKPRKIKSEQLDLVDEISEKEWIHRKQFWLFVSLILTVG
                     LSLVFYFYRYWSQPHLSSSVSPAPTVPFNLEIDDQNEWSVYVRSGDFVYQKNFTDQTA
                     VESAVNFLNQTDPIADSFLYKAIPKGVVVRQIITSRAGSYQGYFRLSIPGREIYILIR
                     ITGQNNLDFSIELIPGVVEKIYWWLV"
     gene            complement(1912..2805)
                     /locus_tag="PK131_01455"
     CDS             complement(1912..2805)
                     /locus_tag="PK131_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPD44827.1"
                     /translation="MKKPEKNLNQSMRKAFGETILKLATINKNLYVVSMDLKSSLSLE
                     EFADVFPDRFIECGLAEANAAGVAAGLAKAGKIVFLCSFACFSPAINWAQIRQSICEN
                     NCPVKIIGSHAGLLSGNLGASHQMLEDVALMRVLPHMEVFAPLDANEVRSLIPVVYQS
                     PSPAYIRLVRPETPVYSSQPFTIGKSNVLQMGNQITIVGYGPILFQAFNFKEAEIINC
                     SSLKPLDEETILKSVRKTKKIIVLEDHQQNGGLGEAIAILILKAGIKCNFRHLAVHDR
                     FGQSGNPEELYNFYGLHLDRL"
     gene            complement(2765..3514)
                     /locus_tag="PK131_01460"
     CDS             complement(2765..3514)
                     /locus_tag="PK131_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPD44828.1"
                     /translation="MLRLDLLDLIYRNGGHIGGSLSSLDLIETVYTSGLFDFDQDHFI
                     LSAGHLAPALYVVLAAIGRFPREWLNNYSQFGSPLQGHTALGLPGVEYAAGSLGQGLS
                     FAAGLALGNRKMKVICLTTDGEQQEGQIWEAAAFANKYRLNNLINLIDVNSLQIDGST
                     DEIMPLGSLIDKYSQFGWAVEEIDGHDLKAIKTTLKKAKQSNQPVGIVAHTILGKGIS
                     FMENNFKYHDVKSLSQDLYEKARKELESIDA"
     gene            complement(3501..>4167)
                     /gene="ispH"
                     /locus_tag="PK131_01465"
     CDS             complement(3501..>4167)
                     /gene="ispH"
                     /locus_tag="PK131_01465"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPD44829.1"
                     /translation="IVNSTNDLPKGSILIIRAHGAAPAVHRLKNLIIVDATCPLVKKV
                     HDEVRRLKNKNIIYLVSSRDHDEAVGVLAEGSNITAYTLVEFNKIKADKNSVLITQTT
                     LSVQETEEFIKKLKKKYPMLKVLPHICPATTDRQRAVKQLAEICDLILIVGSHESSNS
                     QRLYETAKSSGKPAYIVDHVGEIKRSWLKNCHTVGLSSGASTPEDILQSVYNYLTNLY
                     ASS"
BASE COUNT         1431 a          825 c          697 g         1214 t
ORIGIN      
        1 taaacccatt atgattacta taattgatat aaatggcaac tatatgtttc agcaatatta
       61 ttatcactct ttaccagatc aaataaatgt gagtcatttt gaatctggtg gttatacagt
      121 cattgtccat caaggaaata atattcaaca atccaaattt tctaaaatat agattatgtt
      181 tcagcagtct agttgatcta gactgctgat ttcttcttta atttaatgta tatttggtat
      241 tttatatcta taatgcgaaa tgcaataatt ccaacaatat gtaaaaactc ctatttgcat
      301 tttatttaaa agtagtactt taccaatgtg tttaaagtaa tataaaaaat tgatttatct
      361 ttgaataatt gaaaaatttg ggtattgcca ttttatcaca aacttaacca atattttgcc
      421 atatttttac gtgaatcaac cgataacatt aaataaaatc aaaatatctg gatctgatga
      481 aaaaaataaa ttggaaagaa atctttaaat tcttatccgg cgcattcttt gttacagcag
      541 gagccagttg gtattttgcc tggcatcaaa tagatctacc ctttatggga ggaaccatgt
      601 cgcatgaatt tttagtcata cggggatgca tacattttgt tctatttttg ataacgctct
      661 atttcgggta tatcaaaaaa tagtggctaa aaattattga taaaaatgga aaagtattag
      721 aagaagcttc aaatgatcct ggaataaggg ttctttcccg ggaaacagct tttatcattc
      781 aacaaatttt atccgacaat caagcaagga gcttagtctt tggccccaat tcaatgttag
      841 tcatcaaaaa tcaccccgaa gtagccgtta aaacaggaac aacaaacgat atgcgagaca
      901 attggactat aggctatact cccgattacg tcgtcttaac ctgggttggt aacaataata
      961 atgctcgaat gggtggttta gtttccgggg taactggagc tgcccctatc tggcatgaaa
     1021 ttactactca attattaaag gataaagaaa ttaaaaaacc aaccaaacca gcaggtatcg
     1081 ttggccaatc ggtatgtaat ctaaccggtg gcgctatccc ccctgaaggc tgtgaatccc
     1141 attttgaata ttttaaaaag gaatttctac cgccgatcat taaaccaagc cgacaaaacg
     1201 tcctcatcga taaggatagc ggtcgaattg tcaaaccaga agatcataaa cctaacaccc
     1261 aaacggaaga acatttagtt tttcccgacg ccacgggaga acttatatgt cttgactgtc
     1321 ttattgtcga accaacccct acccctttaa actaaccacc aataaatttt ctctactacc
     1381 ccgggaatta attcaatgga aaagtcaaga ttattttggc cggtaattct gattaaaata
     1441 taaatttccc taccgggaat agataaacga aaataaccct gataagagcc agcccgggaa
     1501 gtaattatct gccgaactac tacccctttg ggaattgctt tataaagaaa actatcagca
     1561 atggggtcag tttgattgag aaaattaaca gcactttcaa cagcagtttg gtcagtaaaa
     1621 tttttttgat aaacaaaatc cccagatcgc acataaacgg accattcatt ttggtcatca
     1681 atctccaaat taaatggaac agttggggct ggtgaaactg aggaagataa atggggttga
     1741 gaccaataac gataaaaata aaaaactaaa ctcaaaccaa cggttaaaat caaagaaaca
     1801 aaaagccaga attgttttcg atggatccac tctttttccg aaatctcatc aactaaatct
     1861 aattgttcag atttaatttt tctgggttta attgttttgg gcaacataac tttaaagccg
     1921 atctaaatgt aaaccgtaaa aattataaag ctcttccgga ttacctgatt gaccaaaacg
     1981 atcatgaaca gctaaatgac ggaaattaca tttaattccc gcttttaaaa ttaaaatggc
     2041 tatagcctca cccaaaccgc cgttctgttg gtgatcctct aaaacaatta ttttttttgt
     2101 ttttctaacc gatttcaaaa ttgtttcttc atctaaaggt tttaacgatg aacaattaat
     2161 tatttctgcc tctttaaaat taaaagcttg aaataaaatc ggtccataac caacaatagt
     2221 gatctgattg cccatctgta aaacattaga tttaccaata gtaaacggtt gagaagaata
     2281 gacaggcgtt tccggccgaa ccaaacgaat ataggccgga gaaggagatt gataaacaac
     2341 tggaattaaa gatcgaacct cattggcatc caagggagca aaaacctcca tatggggtaa
     2401 aactcgcatc agcgccacat cctctaacat ctgatgggat gcccctaaat tacctgataa
     2461 aagaccggca tgggacccaa taattttaac cggacaatta ttttcacaaa tcgactgacg
     2521 aatttgagcc caattaattg ctggcgaaaa acaagcaaaa gagcataaaa aaacaatttt
     2581 gcctgccttc gccaagccag cagcaacacc agcggcatta gcttcagcca aaccgcattc
     2641 gataaagcgg tcaggaaaaa cgtcggcaaa ttcttctaaa gacagggacg attttaaatc
     2701 catgctcacc acataaagat ttttgttaat tgtagccaat tttaaaatcg tttccccaaa
     2761 ggccttacgc atcgattgat tcaagttctt ttctggcttt ttcataaaga tcctggctta
     2821 aacttttaac atcgtggtac ttaaaattat tttccataaa agaaattcct ttacccaaaa
     2881 tagtatgggc aactatgcca accggctgat tcgattgttt tgccttcttt agggtagttt
     2941 taatagcttt taaatcatga ccatcgatct cctctactgc ccaaccaaat tgagaatatt
     3001 tatctatcag cgaacccaaa ggcataatct catcggtaga accatcaatc tgcagactat
     3061 taacatcaat aagattaatt aaattattca atcgatactt attggcaaag gcagccgctt
     3121 cccaaatttg accctcttgt tgttcaccgt cggtagttaa acaaatcacc ttcatcttcc
     3181 tatttcccaa ggctaaccca gcagcaaaag ataagccttg acccaaagaa ccagcagcat
     3241 attccacgcc gggaagaccc aaagccgtat gaccctgaag aggagaacca aattgactgt
     3301 aattattgag ccattctcga ggaaatcgtc cgatggccgc cagaacaacg taaagggccg
     3361 gggctaaatg accggccgat aaaataaaat gatcctgatc aaaatcaaac agtccactag
     3421 tgtacactgt ttcaatcaaa tccagactag ataaagaacc gccgatatga ccgccattac
     3481 ggtaaattaa atcaagtaaa tcaagacgaa gcatataggt tagtaagata attataaacc
     3541 gactgaagaa tatcttccgg agtagaagca ccagaagaaa ggccaacagt atgacaattt
     3601 ttaagccagc ttctttttat ttccccaaca tgatcaacaa tatacgctgg tttaccggaa
     3661 gatttggctg tctcataaag tctctgggaa ttggaacttt catgagagcc gacaattaaa
     3721 attaggtcgc aaatctcagc caattgcttc actgctcttt gacgatcggt agttgctggg
     3781 caaatatggg gtaaaacttt caacataggg tattttttct ttaatttttt tataaactct
     3841 tctgtttcct gaactgacaa agttgtttgg gtaattaata ctgaattttt atcagcttta
     3901 attttattaa attcaaccaa cgtataggca gtaatatttg agccttcagc caaaacacca
     3961 acggcttcgt cgtgatcacg actggaaact aaataaataa tatttttatt ttttaaacga
     4021 cgaacctcat cgtgaacttt tttaaccaaa ggacaggtgg catcaacaat aatcaaattt
     4081 tttaaacggt ggactgccgg cgctgcccca tgagcccgaa taatcagaat tgaacctttt
     4141 ggtaaatcat tagtagaatt aacaatt
//
LOCUS       DAOOIE010000035         4130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_16829_length_4130_cov_3.594110, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000035 DAOOIE010000000
VERSION     DAOOIE010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16829_length_4130_cov_3.594110"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="PK131_01470"
     CDS             complement(<1..207)
                     /locus_tag="PK131_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44830.1"
                     /translation="MLIIKKALKKDTDFILQITRENMSSLLVWNDEKFLNNLKIKNIY
                     IVFKDSKKIGLVDCEKIKGNLYIHN"
     gene            513..1391
                     /locus_tag="PK131_01475"
     CDS             513..1391
                     /locus_tag="PK131_01475"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotide-binding protein"
                     /protein_id="HPD44831.1"
                     /translation="MKILGSGLSGLVGSRVVELFKNSFTFVDFSLASGISILEPRQLE
                     EAIAKNSDFDCFLHLAAFTDTNQAWAQNGDENGLCYQLNVIGTRNIVNLCKKYNKYLI
                     NISTDFVFDGKKDGAYTEDDAPNPIEWYGQTKYLAEQEVLNSGIPSSIVRISFPYRAN
                     FGDKKDLVRKAIDGLKTNSLPPMFTDQIITPTFVDDIALGLKYFFENKPTGIFHLVGS
                     SSQSPYDMGQLIAKIWGFDPQLIPQTSLKAYRETLPANSRPWQKNLAISNDKVRALKI
                     AMKTLEEGLRAMKEQM"
     gene            complement(1538..2680)
                     /locus_tag="PK131_01480"
     CDS             complement(1538..2680)
                     /locus_tag="PK131_01480"
                     /inference="COORDINATES: protein motif:HMM:NF005560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPD44832.1"
                     /translation="MKKKIAIIGGGIGGLTVGYFLKKKNYEVTVYEKEKDLGGLMGSF
                     EINGKKIEKTYHHIFASDTEIINLIKELGLDKQLIWRNENMAVIYQNKVYAFNSPIDL
                     LRFSPLDLIDKVRLGLVSIYLQKENNYQKFEKIKAYQWIKKYFGKNVYRVLWQPLLKG
                     KFGKDYKEINMTWFWARIHYRGHSKLGYMKGGFGQIINKLSKNLNIIYKNVKAENLKG
                     YDLIIDTSPIKSVKYLGAVDVIFTSKQNLSKYYWHNINDEKAPFVAFIQQTNMIDDYG
                     SHVYYMGGYYPQDHKYFKVKDEIIYQEFFEYLKKIFKEFEAKEIEKRWIFKFPYAQHM
                     PRFAGSSNNQIPRSKYIIHMNFAQIYPQDRGINYAVKQSRELASLL"
     gene            2748..>4130
                     /locus_tag="PK131_01485"
     CDS             2748..>4130
                     /locus_tag="PK131_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPD44833.1"
                     /translation="MEAIKITKNRDVAIEKVRNIGIIAHIDAGKTTTTERILFYTGKI
                     HKIGIVDEGTTTTDFMIQEKERGITIQSACITTFWKNHRFNIIDTPGHVDFTAEVERS
                     LRVLDGAVMVFDGNAGVQAQSETVWRQADRYGVPRIVFINKMDKIGSDINLVLKSIRE
                     KLRVTAVPVVYPIGKEHEFMGVVDLMTRKMLVWDQDEQGINFTTKELNPEDKDKIEEV
                     RTKMVEAIAGQDEVLMEKFLNDEEIKVEDLKRVLRKATIERKLFPVYCGSAWRNKGIQ
                     PVLDGIVDYLPSPLDLPPVEGYDVETEAKITRKPLREEPLSALLFKVQADSHVGTLSY
                     VRIYSGVLKAGSEVYNATRKTTERIGRLLLMHADKREGIEEGFAGEIVACIGLKESAT
                     GDTLCDRNQPISLLPIRFAEPVISLAIEPQTADDQEKMGQALNKLAIEDPTFKVFYDH
                     ETGQTIISGMG"
BASE COUNT         1238 a          775 c          729 g         1388 t
ORIGIN      
        1 gttatggata tacaaattcc ctttgatttt ctcgcaatcc accagaccta tttttttact
       61 atctttaaag acaatataaa tattttttat tttcaaatta ttaaggaatt tttcatcatt
      121 ccaaaccaac aaactagaca tgttttcccg agttatctgt aaaataaaat cggtatcttt
      181 ttttaatgct tttttaataa tcaacataat aaacataata aacaatatgg gtcagctcct
      241 agaatattat tatttaatac catagctcta gctctacagc caccacaaac atatcgatcg
      301 acacactttc cgcatttacc atcaaaatct ctctttatta tttttactaa aaccttatta
      361 ttaaccctat tttcccaaat attttttaaa gaatcggtta aaatattaac caatgatagt
      421 atgaaataat cttcctgtca attaattata aactcaaaac atctttagcc aacaaatagc
      481 tagagaatag ctaatttttg ttataattct ttatgaaaat tctcggtagt ggtttgtccg
      541 gcttggtcgg ttcgcgggta gtcgagttat ttaaaaacag ctttactttt gttgattttt
      601 ccttggcttc gggaatttct attttggaac cccggcagtt ggaagaagct attgctaaaa
      661 attccgactt tgactgtttt ctccatttgg ccgcttttac tgatactaat caggcctggg
      721 ctcaaaacgg tgacgaaaat ggcctttgtt atcagctaaa tgttatcggc acccgcaata
      781 ttgtcaattt atgcaaaaag tacaataaat atttgatcaa tatttccacc gattttgttt
      841 ttgatggtaa aaaagacggt gcctataccg aagacgatgc ccccaatccc atcgaatggt
      901 acggccaaac caaatatctt gccgagcagg aagttttaaa ttccggtatt cctagtagta
      961 ttgtccgtat ttcctttcct tatcgcgcca attttggcga caaaaaagat ttggtccgaa
     1021 aagccatcga cggcctcaaa accaacagtc tgccgccgat gtttaccgat caaattatca
     1081 ctccgacttt tgttgatgat atcgccttgg gccttaaata tttttttgaa aataaaccta
     1141 ctggtatttt ccatttagtc ggttcttcca gtcaatcacc ctacgatatg ggccaattaa
     1201 ttgctaaaat ttggggattt gatccccagc ttattcccca aactagtctt aaggcatatc
     1261 gagaaactct gccagctaac tcccgtccct ggcagaaaaa tttagctatc agcaatgata
     1321 aagtcagggc tttgaaaatt gcgatgaaga ctttggagga agggttgagg gcaatgaagg
     1381 aacaaatgta agtcctagac cgcttttgtc cgatttaaga cagtgttgtt caactcaagg
     1441 cgaggaaacc tacctgccgg caggctggcc tcgcctttac tatttgattt ttaatacatt
     1501 tcggaatttc gctggataaa cgtagtttgc tgtgttttta cagtagtgat gccaactctc
     1561 ttgactgttt gactgcgtaa ttaattcccc gatcctgcgg gtaaatttgc gcaaaattca
     1621 tgtgaataat atatttgctt cttggaattt ggttattgga acttcccgcg aagcggggca
     1681 tgtgttgagc gtacggaaat ttaaatatcc accttttttc aatttctttt gcctcaaatt
     1741 ctttaaatat tttcttcaga tattcaaaga attcttggta aattatttca tcttttactt
     1801 taaaatattt gtgatcttgt gggtaataac cacccatata gtaaacgtga ctaccataat
     1861 catcaatcat atttgtctgt tgaataaaag caacaaatgg tgctttttcg tcattaatat
     1921 tatgccaata atactttgat aagttttgtt tactggtaaa tattacatct actgctccta
     1981 aatattttac cgattttatc ggcgatgtat cgattatcag atcataacct tttaaatttt
     2041 ctgcctttac atttttataa attatattta gatttttgga caatttgtta atgatttgtc
     2101 cgaaaccacc tttcatatat cccaattttg aatggcctcg gtaatgtatt ctggcccaaa
     2161 accaggtcat atttatttct ttataatcct tgccgaactt tccctttaga agtggctgcc
     2221 ataaaactcg ataaacattt ttgccaaaat attttttaat ccattggtaa gctttaattt
     2281 tttcaaattt ttggtaatta ttttcttttt gtaaataaat tgacaccaaa cctagccgaa
     2341 ctttgtcaat taaatccaac ggcgaaaacc ttaataaatc gattggtgaa ttaaaagcgt
     2401 agaccttgtt ttggtaaatc acggccatat tttcattccg ccaaatcagt tgtttatcta
     2461 accccaattc tttaattaga ttaataattt ccgtatcact ggcaaaaata tgatgatatg
     2521 ttttttcaat ttttttcccg ttaatttcaa aactccccat taaaccacct aagtctttct
     2581 ccttttcata taccgtcacc tcataatttt ttttctttaa aaaatatcca acagttagtc
     2641 cgccgattcc tccgccgatt atagcaattt tctttttcat gtttctgtat aatctatgat
     2701 atgatactat gtttcaatta ctaattacta attactaatt actaactatg gaagcgatta
     2761 aaataactaa aaaccgcgac gtcgcgatag aaaaagtccg taatattggt attattgccc
     2821 atattgacgc cggcaagacg acaactaccg aacggatttt gttttatact ggaaaaatcc
     2881 ataaaatagg tatagttgat gaagggacaa cgaccactga ttttatgatt caagaaaaag
     2941 aacggggaat tactattcaa tccgcttgta ttacgacttt ttggaaaaat caccgtttta
     3001 atattattga tactcccggc catgtcgatt tcaccgctga agtggagcga tccctccgag
     3061 ttttggatgg agcggtaatg gtttttgatg gcaatgccgg tgttcaagct cagtcagaaa
     3121 cagtttggcg tcaggctgat cggtatggag tgccccgaat cgtttttatc aataaaatgg
     3181 ataaaatcgg ctccgatatt aatttagttc ttaagagtat cagggaaaaa ttaagggtaa
     3241 ctgcagttcc ggtagtttac ccaattggta aagagcatga atttatggga gtggttgatt
     3301 taatgacccg gaaaatgctg gtttgggatc aagatgaaca ggggattaat tttaccacta
     3361 aggaattaaa tcctgaagat aaggataaaa ttgaagaagt tcgaactaaa atggtcgaag
     3421 caatcgctgg ccaggacgaa gttttaatgg agaaattttt aaacgatgag gaaattaaag
     3481 ttgaagattt aaaaagagtt ttaagaaagg caactattga aagaaaactt tttccggttt
     3541 attgtggttc ggcttggcga aataaaggca tccaaccagt tttggacggt atcgtcgatt
     3601 atttaccgtc accgttagat ctgcccccgg ttgaaggtta tgatgtggaa actgaggcga
     3661 aaattacccg caagccttta cgggaagagc cgttatcagc ccttttattt aaagttcagg
     3721 ctgattctca tgtgggaact ttatcctatg ttaggatata ttccggtgtt ttaaaggctg
     3781 gttctgaagt ttataatgcc acccgaaaaa ctaccgaacg aattggccgg ctgcttttga
     3841 tgcacgccga taaacgggaa gggattgagg aaggttttgc tggagaaatt gtcgcttgta
     3901 ttggtttaaa agaatcggcc accggtgaca ctctctgtga tcgcaatcag cccatttctc
     3961 ttctgccgat tcgttttgcc gaaccagtta tttctctggc tattgaaccc caaacagccg
     4021 atgatcagga aaaaatgggt caggctttaa ataaattggc aatcgaagac ccgactttta
     4081 aagtttttta tgaccatgaa accggccaaa ccattatttc cggtatgggt
//
LOCUS       DAOOIE010000036         4101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_16968_length_4101_cov_3.091448, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000036 DAOOIE010000000
VERSION     DAOOIE010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16968_length_4101_cov_3.091448"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="PK131_01490"
     CDS             complement(<1..346)
                     /locus_tag="PK131_01490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD44834.1"
                     /translation="MKISVVVTNWNGLPLLKKYFEEIVKTSPEAEEIIFADDASEDNS
                     VAYIKSLQTKYPKIKIIASKKNNGFGINTNQAVKRATGKLIVLLNNDIKPQPNYITNC
                     LKHFQNPKVFGVG"
     gene            complement(343..1521)
                     /locus_tag="PK131_01495"
     CDS             complement(343..1521)
                     /locus_tag="PK131_01495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPD44835.1"
                     /translation="MLAGIYDPYLDTLGGGERYCLTVAEILAKHGYHVDVFWSGDSQI
                     LNKAQDRFSLNLKNINLVPDIFDIPPQTINPIHNRHFLHRLIKHSHSYSKFDEKIKKM
                     IQKFRITRQYDVFFFLSDGSSPFLFGKKNILHFQVPFSQETLTTWPLIKPIKTLFLAK
                     IICNSQFTAKFIQPYFSKKIDVVYPPVDITKFSPADNKKNLILSVGRFDNILNAKKQD
                     ELIDAFRHLVKIHRLANWNLIFAGGSLQQPTSNYFLQYLIKKSENLPIEFIVNPSFSQ
                     LKELYSKGKIYWHAAGFGVDENSHPEETEHFGMTIVEAMASGLVPVAVAKGGIPEIID
                     DSINGFLWKSLKGLTSKTLRLIKDPKLFNQMQAKALLKAKQFSKENFEETFIRLVKIK
                     "
     gene            complement(1596..2381)
                     /locus_tag="PK131_01500"
     CDS             complement(1596..2381)
                     /locus_tag="PK131_01500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPD44836.1"
                     /translation="MARNLSLIVIAGNEEEMIADCLKSANFAEEIILVAANSNDQTLT
                     IAKKIITKIKIIKVNDEYNKHFAKWRNLGYQAATKKWLFYLDADERISPFLRDEILKV
                     TSTGRYTHYAVPRANFFLGQRVKYGGTYPDYVKRLFKREKFKGYQGILHEEPIVTGQI
                     GYLKNDLLHYTHRSLSSMLQKSIAWTDMEAQALYRAKHPPVTWWRILRMFFTKLHSRL
                     ITQQMFRDGTVGWISSFFEAFDTFLIYARLWERQQQLKIKNYE"
     gene            complement(2360..3292)
                     /locus_tag="PK131_01505"
     CDS             complement(2360..3292)
                     /locus_tag="PK131_01505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPD44837.1"
                     /translation="MAKIAVIVLNYRQPTLTLATVRSLLKISLEEASYHLFLVNNNCL
                     DDSLNIFEEKLADNPKITLVSTGSNLGYAGGNNFGIKLALKEGYDYCLIINNDVLVDP
                     KFLQYLYETAKNTVTPAIIGPKIYFAKGYEFHHDRYQNKELGRVIWSAGGLIDWKNIY
                     ASNLGVDQVDRGQFDQINSNLDFITGCCLLIPKEIFHKIGFFDERYFMYLEDVDFCYR
                     AKLQGFKIIYQPKSSIWHINAGSSSSGSSLHDYFITRNRLLFGLKYARPRSKLALTRQ
                     AVRLLFTGRKWQKRAVIDYYLKHLGKGSWREISL"
     gene            complement(3292..3639)
                     /locus_tag="PK131_01510"
     CDS             complement(3292..3639)
                     /locus_tag="PK131_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44838.1"
                     /translation="MAEYKIKVSDFILKPIQEFNSRFFMAEGYKDGFHGLIFCLLQAI
                     AVALVYIRLWEKEGAQDKPLPKSSFVSSSQESLFEYDYWFTRYFQKEYSRNIFKNLII
                     KIRLLFDRLSKNF"
     gene            complement(3891..>4101)
                     /locus_tag="PK131_01515"
     CDS             complement(3891..>4101)
                     /locus_tag="PK131_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HPD44839.1"
                     /translation="STLSGRKRALNVKGAYKIEKGADVKGKRIILIDDVFTTGATTME
                     VSKVLKRAGAKYVLVATVAKSIQVF"
BASE COUNT         1361 a          702 c          726 g         1312 t
ORIGIN      
        1 aaccaactcc aaaaactttc ggattttgaa agtgcttgag acaattggta atataatttg
       61 gctggggttt aatatcgtta tttaataaaa ctattaattt accggttgcc cttttgaccg
      121 cctgattagt gttaattcca aagccgttat tctttttaga agctataatt tttatttttg
      181 ggtatttagt ttgaagagat ttgatataag cgacagaatt gtcttctgaa gcatcatcag
      241 caaaaattat ttcttcagct tcgggactgg ttttaacaat ttcttcgaaa tactttttca
      301 acagcggtaa accgttccaa ttagtgacaa caactgatat tttcatttaa ttttaactag
      361 tctgataaat gtttcttcga aattttcttt agaaaattgt ttggctttta ataaagcctt
      421 agcttgcatt tgattaaaga gtttaggatc cttaattaaa cgaagagttt tactggtaag
      481 tccctttaaa cttttccata agaagccatt aattgaatcg tcgattattt caggaatacc
      541 ccctttggca accgctaccg gcacaagacc ggaggccatg gcttcgacaa tagtcattcc
      601 aaagtgctcc gtttcttctg gatgagaatt ttcatcgaca ccaaagccgg cagcatgcca
      661 ataaattttt cctttagaat aaagctcttt taattgacta aaagagggat taacaatgaa
      721 ttcgatagga agattttctg actttttgat aagatattga aggaaataat tagaggttgg
      781 ttgttgtaaa ctaccgccag caaaaattaa attccaattc gccaatcgat gaatcttaac
      841 taaatgtcga aaggcatcaa ttaattcgtc ctgttttttg gcattaagaa tattgtcaaa
      901 acgacctaca gaaagaatca gatttttttt attatcagcc ggggaaaatt tggtaatatc
      961 gaccggagga taaacaacat caattttttt agaaaaataa ggttggataa atttagcggt
     1021 gaattgggaa ttacagataa ttttcgctaa aaaaagggtt ttaattggct tgattaaagg
     1081 ccaagtcgtt aaagtttcct gactgaaagg aacctgaaaa tgaagaatat ttttcttacc
     1141 aaaaaggaag ggactactgc catcagataa aaaaaagaaa acatcatatt gccgagtaat
     1201 ccggaatttt tgaatcattt tcttaatttt ttcgtcaaat ttggagtagg aatgagaatg
     1261 tttaattaag cgatgaagaa aatgacgatt gtggatggga ttgattgtct gggggggaat
     1321 atcaaaaata tctggaacca aattgatgtt tttaagattc agtgaaaaac gatcttgagc
     1381 cttattaagg atttggctgt cgcccgacca gaaaacatca acatgatagc catgtttagc
     1441 tagtatttca gctacagtca ggcaatatcg ttctccccct cccaaagtat caagataagg
     1501 atcatagata cccgccaaca tatttaattt aatttaattt aattaaaaat tacgaattac
     1561 gaattaaaaa atacatcctc gaatttttca gtttttcatt cgtaattttt aatttttaat
     1621 tgttgctgtc tctcccacag tctagcataa attagaaaag tatcaaaagc ttcaaaaaaa
     1681 ctgctaatcc aaccgactgt cccatcccga aacatctgtt gggtaattaa acgactatgt
     1741 aatttagtaa aaaacattct taagattcgc caccaggtta ccggtggatg tttagctcga
     1801 taaagggctt gagcttccat atcggtccag gcaattgact tttggagcat agaagataaa
     1861 gatcgatgag tataatgaag aaggtcattt tttaaatagc caatttggcc ggttactatt
     1921 ggttcttcgt gtaaaatacc ttgatatccc ttgaattttt ctcttttaaa aagacgcttg
     1981 acatagtcag gataggtacc gccatatttt acccgttggc cgagaaaaaa attagctcga
     2041 ggaacagcat aatgagtata acggccggta gaagttactt ttagaatttc gtcccttaaa
     2101 aatggagaaa ttctttcatc agcatcgaga taaaaaagcc attttttagt ggccgcctga
     2161 taacccagat tgcgccattt ggcaaaatgt ttattatatt catcattaac tttaataatt
     2221 ttaattttag taatgatctt tttcgcgata gttaaagttt gatcgttgga attggcggca
     2281 actaaaataa tttcttcggc aaaattggca ctcttaagac aatcggcaat catttcttcc
     2341 tcattgccgg ctatgactat taaagagaga tttctcgcca tgaacctttc ccaagatgtt
     2401 ttaaataata atctataaca gcccttttct gccattttcg accagtaaaa agaagtctaa
     2461 ctgcttgacg ggttaaagct agtttagaac ggggtcgagc atatttcaga ccaaaaagaa
     2521 ggcggtttct ggttataaaa taatcatgta aagaactacc gctggaggat gagccggcgt
     2581 tgatatgcca gatggatgat ttaggctgat aaataatttt gaacccttgt aatttggccc
     2641 gatagcagaa gtcaacatcc tccaaataca taaaatatct ttcgtcaaaa aacccgattt
     2701 tatggaaaat ttccttaggg ataaggagac agcagccagt aatgaaatct aaattagaat
     2761 tgatttgatc aaattggcct cggtcaacct gatctactcc tagattggaa gcataaatat
     2821 ttttccaatc gattaggccg ccagctgacc aaataacacg acctaattct ttattttgat
     2881 accgatcatg gtgaaattca tagcctttag caaaataaat ttttggtcca ataatggctg
     2941 gagtaacagt gtttttggcc gtttcgtaaa gatattgaag gaatttggga tcaaccagaa
     3001 cgtcattatt aattatcagg cagtaatcgt aaccttcttt taaagccagt ttaatcccaa
     3061 agttattacc accggcataa cctaaattac tgccggtaga aactaaggta attttgggat
     3121 tatcggctaa tttttcttca aaaatattaa gagaatcatc gagacaatta ttattaacta
     3181 aaaaaaggtg ataagaagct tcttccaaac ttatttttag aagagaacgg acagtcgcta
     3241 acgttaaagt tggttgtcgg taattaagga caataacggc aattttggcc attaaaaatt
     3301 tttacttaat ctatcaaata aaagacgaat tttaataatc aaatttttaa aaatattccg
     3361 agaatattcc ttctgaaaat aacgagtaaa ccaataatca tattcaaata agctttcttg
     3421 actgctggat acgaaagaac tcttgggcaa aggtttatct tgagcacctt ccttctccca
     3481 caggcggata taaaccaaag ctactgctat agcttgaagg agacagaaaa ttaagccatg
     3541 aaaaccgtct ttgtaacctt cagccataaa aaaacgactg ttaaattcct gaattggttt
     3601 gaggataaaa tcactaactt taattttata ttctgccatc cgaagttcat cagattgaat
     3661 tcccgcataa cgaagggccc tagttataaa aatagtgaca gtgggataat tattatggcg
     3721 gatggccaat ttttcactat caagaagagt aagacagttg cctcgagcgg tgggttgact
     3781 gtggattaat ttactccaag tgacgtgacc ttttttgaag aaacggatta aataatctgg
     3841 ccagcagcga ccatgacgaa gccatttacc taaaataata ttttcaatta ttaaaaaact
     3901 tgtatagatt ttgcgacagt tgcaacaaga acatatttag ctccggctct ttttaaaact
     3961 tttgaaactt ccatggtggt tgctcctgtt gtaaaaacat catcaattaa aataatacgc
     4021 tttcctttta catctgcacc tttttcaatc ttatacgcac cttttacatt aagtgctctt
     4081 ttgcgacctg atagggtact t
//
LOCUS       DAOOIE010000037         4048 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_17232_length_4048_cov_3.481593, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000037 DAOOIE010000000
VERSION     DAOOIE010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17232_length_4048_cov_3.481593"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1149)
                     /locus_tag="PK131_01520"
     CDS             complement(<1..1149)
                     /locus_tag="PK131_01520"
                     /inference="COORDINATES: protein motif:HMM:NF021570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HPD44840.1"
                     /translation="MLTDFKYIFLWWLVVFVFGASILPFTVNLFRRFFNKGYIFSKII
                     AIAFVSYCLFVLGVFKLVPFTAPSAFIFLLLLIFVNYYCFLFKKKKLNNFGRLIKNNY
                     RVFLMEESMFLAILIFWSFIRGYNPTIEGLEKFMDWGFVNSILRSRFMPPADMWFAGK
                     PINYYFFGHLIFALLTKVSGISSAITYNLAIATVCALTFVSGFSLTANFIHQFGSKKI
                     NYSFVIIGGLISALFLTFGGNLHPLYKIARLNIKQNNGQLILSSRAIKQAVANYWYPD
                     ATRFIGFDPETNDKTIHEFPIYSFVVADLHGHMNDIPIVLLFIALLLALIPQRVKLYH
                     WSLMIGGGLVLAWAYMTNAWDFAIYGLLFALIYFLVHLDLKKLIINGVG"
     gene            complement(1143..1706)
                     /locus_tag="PK131_01525"
     CDS             complement(1143..1706)
                     /locus_tag="PK131_01525"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPD44841.1"
                     /translation="MEKARRLLFWQPQKDYLKVFLTTKFLNYLLIFLNYFIWAFLFYI
                     ACLLVKNNFFSLGQILVALVISEIGERWIKSKVYWRRPFLNRHDSIPQGLVVNWYRTG
                     SFPSGHTAKAVFFFFLVEHYGVFNPILYWLIIIPLLLFRVVVGFHYPIDLLGGMVIGL
                     ISYFFSLLIVLTPVVSNLIEKIFNFLC"
     gene            1750..2865
                     /locus_tag="PK131_01530"
     CDS             1750..2865
                     /locus_tag="PK131_01530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPD44842.1"
                     /translation="MKVLISTPYYLPNVSGITFYVQILAEELAKRGHQVTILTSQHLK
                     KLKLKETINQVSIIRLKAPLKISKGVIMPSFFWIAVKESKNNDIIIGNLPQAEAFWIS
                     WWAKIWRKKVILIHHTDLSFWPGIKNKIIDSGVFICHCLAALSTDYLVPYTDDYARHS
                     YFLKHFLKKVKSIYPPVKFEPSKDKKQIKNKYIVGFCGRIAKQKGLETLIESCDYLDQ
                     KIGSANYLIKLAGPMEIIGEKYYDYLKEKYYRRLKNNFIFLGGIDREKLADFYHSIDL
                     LVLPSNDSLESFGWVQIEAMICGTPCVATNLPGMRVPILKTKMGELFEKNNSQQLAEK
                     IVKVLKNGKQYYHKLGKNALQQFDYQKTIDHYEKLFR"
     gene            2804..3640
                     /locus_tag="PK131_01535"
     CDS             2804..3640
                     /locus_tag="PK131_01535"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPD44843.1"
                     /translation="MPYSNSIIKRQSIITKNYLDNEIDSAFRRRAKIILNNLDIKGKM
                     SVLEIGCGRGFYLKILRDLYPTAVITGIDINPLYIHQVKHNIKNVNVFQADAKTLPLA
                     DNSFDWIIAEILEHIDNDQKALKEMYRVLKPKGKAIITVPNANYPIYWDPLNWSLERL
                     FKKHIKATNWYLGGIWADHQRLYTDQQLVEKITGAGFKIIKKWSTTHYCLPAASFWFY
                     GLGKHLVEKGMANNLNRFNFTRKPNYLSKTILKLVNIFDKMNRDNQNYSSSVNLICQI
                     EK"
     gene            3622..>4048
                     /locus_tag="PK131_01540"
     CDS             3622..>4048
                     /locus_tag="PK131_01540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPD44844.1"
                     /translation="MSNRKIKTNCIVAGILLIGIFFRFYQLDYLPADIFGDISENLNH
                     IADIIKGNYKIIYGFDAREGLLFYLTAPFAKIFGNNYLTLKTITASIGVLAILAEYYF
                     VKELTNERVALIAAFLVAVSKWPIIFSRIGFRNILTPMLT"
BASE COUNT         1570 a          678 c          649 g         1151 t
ORIGIN      
        1 ccccacccca ttaataatta atttctttaa atccaaatgg actagaaaat aaattaaggc
       61 aaataaaaga ccataaatgg caaaatccca ggcattagtc atataagccc aagctaaaac
      121 tagaccgccg ccaatcatta atgaccaatg atacaattta actctctgag gaataagggc
      181 taaaagaagg gcaataaata gtaaaacaat tgggatatcg ttcatatggc cgtgtaaatc
      241 agctaccaca aaactataaa ttgggaattc atggatagtt ttatcgttag tctcaggatc
      301 aaaaccaata aatcgggtag catcgggata ccaataatta gcgacggcct gtttaatagc
      361 tcgagaggaa agaattaatt gaccattatt ttgttttata ttcaatcggg caattttata
      421 aagaggatgg agattgccgc cgaaggttaa aaaaagagct gagattaggc cgccgataat
      481 tacaaaagaa taattaattt tcttgcttcc aaattgatga ataaaattgg ctgttaacga
      541 aaaaccagaa acaaaagtta aagcacaaac agtagcaata gccaaattat aggtaatagc
      601 tgaactaata ccagatacct tagttaaaag agcaaaaatt aaatgaccaa aaaaatagta
      661 gttaatcggt tttccggcga accacatatc ggccgggggc ataaatcgag atcgtaaaat
      721 agaattaaca aaaccccagt ccataaattt ttccaaacct tcaatggtcg gattatagcc
      781 ccgaataaaa gaccaaaaaa ttaaaatagc caaaaacatt gactcctcca ttaaaaaaac
      841 ccggtaatta tttttaatta ggcggccaaa attattcaac ttttttttct taaaaagaaa
      901 acagtaataa ttcacaaaaa taagtaacaa aagaaaaata aaagccgagg gagcagtaaa
      961 aggcactaat ttaaaaactc ccaaaacaaa taaacaatag gaaacaaaag cgatagcaat
     1021 aattttcgaa aaaatataac ctttattaaa aaatcggcga aatagattaa ccgtaaatgg
     1081 caaaatcgag gcgccaaaaa caaaaaccac tagccaccaa agaaaaatat atttaaaatc
     1141 agttaacata aaaaattaaa tattttttca attaaattac taactactgg cgttaaaaca
     1201 atcagaagag aaaaaaaata agatattaaa ccaatgacca ttccccctaa aagatcgata
     1261 ggataatgaa aaccaactac cactcgaaac aaaagcaaag gaattattat taaccaataa
     1321 agaatggggt tgaaaacacc gtaatgttcc accagaaaaa agaaaaaaac tgctttagct
     1381 gtatgaccgg aagggaaaga accagtccga taccagttaa ccactaaccc ttgagggata
     1441 gaatcatgtc gatttaaaaa aggtcggcgc cagtatactt tactcttaat ccatctttca
     1501 ccaatttctg atataactaa agccactaaa atttggccta aagaaaaaaa attattttta
     1561 accaaaagac aggcaatata aaacaaaaat gcccaaataa aataattcaa aaaaattaac
     1621 agataattta gaaatttggt ggttaaaaaa actttcaaat aatccttttg gggctgccag
     1681 aataaaagcc gtcgagcttt ttccataatc aattataatc tactaagttg gatttgttat
     1741 aatgaaaaag tgaaggtttt gatttcaact ccatattatt tacccaatgt ttcgggcatt
     1801 accttttatg tccaaattct ggctgaagaa ctagccaaaa ggggccatca agtcactatt
     1861 ttaaccagtc aacatttaaa aaagctgaaa ctcaaagaaa ctattaacca ggtttctatt
     1921 ataagactaa aagctcctct gaaaattagc aaaggcgtta ttatgccttc gtttttttgg
     1981 atagctgtca aagaaagcaa aaacaatgac attattattg gtaatcttcc tcaggcagaa
     2041 gcattttgga tcagctggtg ggctaaaatc tggcggaaaa aggttattct tatccaccat
     2101 actgacctaa gtttttggcc aggaattaaa aataaaataa ttgatagcgg tgtttttatt
     2161 tgtcactgct tagccgctct gagcaccgac tacctcgttc cctacaccga cgattacgct
     2221 cgtcattcat acttccttaa gcactttctt aaaaaggtaa aaagtatcta tccaccagtt
     2281 aaatttgaac caagtaagga taaaaaacaa ataaaaaaca aatatatagt cggtttctgc
     2341 ggtcgaattg ctaaacaaaa aggactggaa actttgatcg aaagctgtga ttatttagat
     2401 caaaaaatcg gttcagccaa ttatttaatc aagctagctg gaccaatgga aataatcgga
     2461 gaaaaatatt atgactatct aaaggaaaaa tattatcgtc gcttaaaaaa taattttatt
     2521 tttttgggcg gtattgacag ggaaaaatta gctgattttt atcactcaat tgatcttttg
     2581 gttctaccat ctaacgactc tttagaatca tttggctggg tccaaattga agctatgatc
     2641 tgtggcactc cctgcgtcgc tactaatcta ccgggaatga gagtgcctat tttaaaaact
     2701 aaaatgggag aattatttga aaaaaacaat agccaacaat tagcagaaaa aatagttaaa
     2761 gttttaaaga atggtaaaca atactaccat aagttaggta aaaatgcctt acagcaattc
     2821 gattatcaaa agacaatcga tcattacgaa aaattattta gataatgaaa ttgattcagc
     2881 ttttagacga cgggctaaaa taattctcaa taaccttgat atcaaaggca aaatgtcggt
     2941 tttggaaatc ggctgtggtc ggggatttta tttaaagatc ttacgagatc tttacccaac
     3001 agcagttatt accggtattg acattaatcc tctttatatt caccaagtaa aacataatat
     3061 taaaaatgtt aatgtttttc aggccgatgc taaaacttta cctttagctg ataattcttt
     3121 tgactggatt attgctgaaa ttttagagca tattgataat gatcaaaaag ctttgaaaga
     3181 aatgtaccga gttttaaaac caaaaggaaa agcaattatt actgtcccca acgccaatta
     3241 cccaatttat tgggacccat taaattggtc gctagaaaga ttatttaaaa aacatatcaa
     3301 agccactaat tggtatttag gcggtatttg ggctgaccat caaagacttt ataccgatca
     3361 acaattggta gagaaaataa caggagcagg ttttaaaata attaaaaaat ggtcaactac
     3421 ccattattgt ttaccggcag ccagtttttg gttctacggc cttggtaaac acttagtgga
     3481 aaagggaatg gcaaataact taaatcgatt taatttcaca agaaagccta attatttaag
     3541 caaaactatt ctcaaattgg ttaatatctt tgacaaaatg aaccgagaca atcaaaatta
     3601 ttcgtcgagt gttaatctaa tatgtcaaat agaaaaataa agacgaattg tatcgtagca
     3661 ggtattttat taattggaat tttttttaga ttttatcagc tcgactatct accagcagat
     3721 atttttggtg atatttcgga aaacttaaac catattgctg atattatcaa aggcaactat
     3781 aaaattattt atggttttga tgccagagag ggattgcttt tctatttgac agcccctttt
     3841 gccaaaatct ttggcaataa ttatctaacc ctcaaaacaa ttacagccag tattggggtt
     3901 ttagcaattt tagccgaata ttacttcgtt aaagaattaa ccaatgaaag agtagccttg
     3961 atagccgctt tcttagtagc agtttctaaa tggccaataa tttttagtcg aattggtttc
     4021 agaaatatct taactccaat gctaactg
//
LOCUS       DAOOIE010000038         3856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_18303_length_3856_cov_2.788214, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000038 DAOOIE010000000
VERSION     DAOOIE010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18303_length_3856_cov_2.788214"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1854
                     /locus_tag="PK131_01545"
     CDS             <1..1854
                     /locus_tag="PK131_01545"
                     /inference="COORDINATES: protein motif:HMM:NF012622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HPD44845.1"
                     /translation="LKREIKSIDGKDCLRMVVGVSGSDGNVPPKSGNLIGFKVKIKGI
                     AASGTSQIEMSDDPSRTFFYTSVGEDVPGKIESRGGVKINFGQASTVPEAVVGLSPTK
                     LPMKIGEAEEVLVKIDASASGRSINAVGIRLCYDDAVKIADRTKDITIAGSGSQGLSK
                     ISEISDVLSVGGTSCLFFYSIVNPSQEGVILPSGAFDMYRIKFTGVKAEEGNIWIDFT
                     NDPDRLNTEISGPCPSPCLPPQDEKFKIGPMEGASSCAFTVEAVPSDEAAVKVALDKN
                     IYNVGDEAIATVSVDTGKFKRQMSTGDFGLCLDKHLAIGNLDQAVTPIKENGILKLYN
                     LQKVNSPDNSDNYCFQFATEADKEQLPSGIFEVAKIKMNAIAAGEGKISWLQDETTKL
                     SGPIAANGDEKDIEIKDYTDASYRVEEIIKSKLIMKFKMTFEEVLATSECAKQQWKTV
                     GVRVMDQEGIVAEDLGASLTPTTTTVVVGEHENKKELQVFEVTAGLVNFQPKGNIFAL
                     VKGPMHLYDKYGVDGQSEEYKLYLGKLNGITSDPATTKVFDFSHYPLLAGDVNGDNKM
                     NAADYSKIKTALANGKKEAVTAVDLNGDCILNSTETVIFIKNINEKNGKMY"
     gene            1829..2305
                     /locus_tag="PK131_01550"
     CDS             1829..2305
                     /locus_tag="PK131_01550"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPD44846.1"
                     /translation="MKRMGKCTKSGFTLVELLVSITIIMVLTVTAVVNYGNAQKNARD
                     AKRKADLEKIRVALELYKQDLGGTYPANYRNSLVGYLDTVPTADPIGCPYFYEQKEMG
                     ASYRLGAHMELERNCTQEKTNPTNCEGQDGGGCDKRCLDPSPSTGKIIFNYVIHSP"
     gene            2302..2913
                     /locus_tag="PK131_01555"
     CDS             2302..2913
                     /locus_tag="PK131_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44847.1"
                     /translation="MKIKQRGLVQIPIIIGLMIVAVAIPVVKGIMNKEKKVSPVVTEP
                     AIGEKEEEKKEGEVRLEPTVFSLKQGEKKKIKLVIDTSSSGRKVDIARVGLCWDQGLK
                     IADFDKDIILDMAYFSDFVSKKLVKLQGKDCADIIVAAQWKTADLKSGKLVAAEINFL
                     GTDKTDGKFTIDQSLTEVSGPANQAGVYKISLGNTDQFHYQVN"
     gene            2922..>3856
                     /locus_tag="PK131_01560"
     CDS             2922..>3856
                     /locus_tag="PK131_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44848.1"
                     /translation="MKQKKIIFLAVLALALGITANLVAKPQETRKKAAGEGICTCQIN
                     NDYCYDALSEFGGKQWNPCKGCTLCRARDLGELLPMRSIAEKFSCRLIGREVSLGEYC
                     QDPELFSRCPELNQIDLCQCCNLEGIDYYWGHRGRCTGADCTGIIPLPEPTDTPGPSN
                     TPTPTSTPTPTLTPTPTPDPTECQCQLDQSHCFADGELRNPCKGCTLCGKRDKFVPLA
                     PIWVQTNYNCPALKRSVSVGEYCENPDWFADCDITINLCQCCNLNGYGSAYQSNWGQC
                     TRADCSGVTLTPTPTSTPTPTSTPTPTGTITPSLCR"
BASE COUNT         1166 a          646 c          969 g         1075 t
ORIGIN      
        1 cttaaacgtg agattaagtc aattgatggt aaagactgct tgcggatggt agtcggtgtg
       61 tcaggatcag atggtaatgt tcctccaaag agtggtaatt tgattggatt taaggttaag
      121 ataaaaggaa ttgccgccag tggtactagt caaattgaaa tgagtgatga tccctcgaga
      181 accttttttt atacctcagt aggggaggat gtcccgggta aaattgaatc gagagggggc
      241 gtaaagatta actttggtca agcttcaaca gttccagaag cagttgtcgg tttgagcccg
      301 acgaagctgc ctatgaaaat tggtgaggcg gaagaggttt tagttaagat tgatgcctcc
      361 gcctccggta gaagcatcaa tgctgttggc atcaggttgt gttatgatga tgcagttaaa
      421 atagccgata ggacgaagga tattactatt gccggcagtg gcagtcaggg tttaagtaaa
      481 atttccgaaa tttcagatgt tctctctgtg ggggggactt cttgcctttt tttctattcc
      541 attgtaaatc ctagtcaaga aggagtgatt cttccatcag gagcttttga tatgtatagg
      601 attaaattta ctggggtaaa ggcagaggag gggaatattt ggattgattt taccaatgat
      661 cctgacagat tgaacactga aattagtggt ccttgcccgt ctccctgcct ccctccgcag
      721 gatgaaaaat ttaaaatagg tccaatggaa ggagcaagct cttgtgcttt taccgtagaa
      781 gcagttccta gtgatgaagc ggcggttaaa gtggccttag ataaaaatat ttataatgtc
      841 ggcgatgagg ctatagcaac agtctcagtt gacaccggga aatttaaacg ccagatgagt
      901 acgggtgatt ttggtttgtg tttagacaaa cacttggcta tcggtaattt agaccaggca
      961 gttacaccga ttaaagagaa cggtattttg aagctatata atttacagaa agtgaatagt
     1021 cctgataatt cagataatta ttgctttcaa tttgccacgg aagcggataa ggagcaatta
     1081 ccatcaggaa tttttgaggt ggctaaaata aagatgaacg cgatagcggc cggggaaggc
     1141 aaaattagtt ggctccagga tgaaactact aaacttagcg gaccaattgc ggcgaatggt
     1201 gatgagaaag atattgaaat taaggattac acggatgctt cttatcgagt agaggagata
     1261 attaagagta aattgattat gaaatttaag atgacttttg aagaggtttt agcgactagt
     1321 gaatgtgcta agcaacaatg gaaaacggta ggggtgagag ttatggatca ggagggaatt
     1381 gtggctgaag atttaggagc ttcacttacc ccaacaacta ctacagttgt agtaggagaa
     1441 catgaaaata aaaaggaatt gcaggttttt gaagtaacag ctgggttggt taattttcag
     1501 ccgaaaggga atatttttgc cttagttaaa ggtccaatgc acctttatga taaatatggt
     1561 gtcgatggcc aaagtgaaga atataagtta tatttaggga agttaaacgg gataactagt
     1621 gatcctgcta ctaccaaagt gtttgatttt agccattatc cacttttggc tggcgacgtt
     1681 aacggtgaca ataagatgaa tgcggcagac tatagcaaaa ttaaaacagc tttagccaac
     1741 gggaaaaagg aagcggtgac ggcagttgat ctgaatgggg attgtatttt gaacagtacc
     1801 gaaactgtga ttttcattaa aaatattaat gaaaagaatg ggaaaatgta ctaagtctgg
     1861 gtttaccctg gtcgagcttc tggtttctat cactattatt atggttttaa cagtaacggc
     1921 agtagttaat tacggtaatg cccaaaagaa cgcccgagat gccaaaagga aggcggattt
     1981 ggaaaaaatt agggtggcat tggagttata caagcaggat ttgggaggaa cttacccggc
     2041 taactataga aattcgttgg ttggttatct tgatactgtg ccaacggccg atcctattgg
     2101 ttgcccttat ttttacgagc agaaagaaat gggtgccagc tatcggctgg gagctcatat
     2161 ggagttagaa agaaattgca ctcaagagaa aacaaatccc accaattgtg aaggtcaaga
     2221 tggtggtgga tgtgataaac gctgcctaga tcctagtcct agcacaggta aaattatatt
     2281 taattacgtt attcacagtc catgaaaata aaacagcgag gtctagttca aatcccgatt
     2341 attattggat tgatgatagt cgcggtagcg ataccagtgg ttaaagggat aatgaataag
     2401 gagaaaaagg tgtcaccggt agttaccgaa ccggcaatag gagaaaaaga ggaagagaaa
     2461 aaggaaggcg aagttagatt ggagccaact gttttttctt tgaaacaagg agaaaagaaa
     2521 aaaattaagt tggttattga tacttcttca agcggccgca aggtagatat tgcccgggtt
     2581 ggtttatgtt gggatcaagg cttaaaaata gccgattttg ataaagatat tatcttggat
     2641 atggcttatt ttagtgactt tgtttctaaa aaactggtca aattacaagg taaagattgt
     2701 gccgatatta tcgtagctgc tcaatggaaa acagccgatt taaaatcggg taagttggta
     2761 gccgctgaga taaattttct tgggacggac aagactgatg gcaagtttac gattgatcaa
     2821 tctttaaccg aggtttccgg accggccaat caggccgggg tctataaaat ttccttggga
     2881 aatacggacc aatttcatta tcaggtcaat taaaaatgct cttgaagcag aaaaagataa
     2941 tctttctggc agttttagcc ctggctttgg gaataacagc taatttggtt gccaaaccgc
     3001 aggagaccag aaaaaaggcc gccggtgaag ggatttgtac ctgtcaaatt aataatgatt
     3061 attgttatga tgccttatca gagtttggtg ggaaacaatg gaatccatgt aaaggttgta
     3121 ctttatgcag agcccgggat ttaggcgagt tattgccaat gagatcaatt gcagaaaagt
     3181 ttagctgtcg tttgattgga cgggaagtga gtttgggcga gtattgtcaa gatcctgagc
     3241 tttttagccg ttgcccagaa ttaaatcaga ttgatttgtg ccaatgttgt aatcttgaag
     3301 gtattgatta ttattggggg catcggggaa gatgtactgg ggctgattgt accgggataa
     3361 ttcccttacc ggaaccgact gataccccgg ggccaagtaa tactccaacg ccgacaagta
     3421 ctccgacccc aaccttgact ccaacgccga cacctgatcc gacggagtgt caatgtcagc
     3481 tggaccagag ccattgtttt gccgacggcg agttgagaaa tccctgcaag ggttgcactc
     3541 tctgcggcaa aagagataag tttgttccat tagccccgat ctgggtccaa accaattata
     3601 attgtccggc cttgaaaaga tcagtttcgg ttggggaata ttgcgaaaat cctgactggt
     3661 ttgctgattg cgatattact atcaatttat gtcagtgttg taatcttaat ggctatggca
     3721 gtgcttatca atctaattgg ggacaatgca cccgggccga ttgcagtggg gtaacgctaa
     3781 cgccgacgcc aacctctact cctaccccaa cttctactcc gactcccacc ggaactatta
     3841 ctccgtctct ctgtcg
//
LOCUS       DAOOIE010000039         3845 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_18369_length_3845_cov_2.944591, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000039 DAOOIE010000000
VERSION     DAOOIE010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18369_length_3845_cov_2.944591"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PK131_01565"
     CDS             <1..237
                     /locus_tag="PK131_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44849.1"
                     /translation="STVKQVITTGLNPADNYWTEAEWQRFWFGSLEDEINLLRTLPQP
                     LYLYHSDRQPDLPFVNDSKCFEKVSWRVYRFKCK"
     gene            458..1456
                     /gene="nusA"
                     /locus_tag="PK131_01570"
     CDS             458..1456
                     /gene="nusA"
                     /locus_tag="PK131_01570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPD44850.1"
                     /translation="MDLKRLPKTEFAQAVAQIAGERNIDPQLILDSIEAGLISAYKRN
                     QREHGIVIPDEDQFEVDLSPESGLFKVFKIEGDKRLEVTPPDFGRIAAQTAKQVIDQK
                     IYEAEKEVIIAEFQKKVGSLVTGVVFRSDPSKLVIGVGKTEAICPKDEQIKGENLPLG
                     AKKLFLIKEIFRDEITGQSEVILSRRDNNFIKELFAREVPEVSNRVVRVEKIARTPGE
                     RTKVAVASSQPGVDPVGSCIGQKGSRIQSVLNELPAEEKVDVILFSKNLNQFIIAALS
                     PANGVRVEAIENGIAQVTVPEDQLALAIGGGGENVRLAGVLTGLEIKIAARKETQS"
     gene            1479..2864
                     /gene="infB"
                     /locus_tag="PK131_01575"
     CDS             1479..2864
                     /gene="infB"
                     /locus_tag="PK131_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPD44851.1"
                     /translation="MFRPPIVTIMGHIDHGKTTLLDKIRSTNVCAKETGGITQHINAY
                     QAKGITFIDTPGHAAFINMRARGAKITDIVVLVIAGTEGVMAQTKECLKLVKEFKLPL
                     IVALNKKDLAGFAPDKVKGQLIEAGFTPEEYGGDVPCLAISAKTGEGIDKLLETIKLL
                     ADLLDLKAEPEAPLLAAVIESCLDKNRGPIATAVVEKGTVKTGDIIYAGSISGKVRAL
                     IDDNGKKIDSAGPSTPVVILGCEAVPPVGSVITEQPQQVATVPAAKLTADSTASNKLK
                     IILKADTQGTLEAIRNCLTDDVLILEAGVGAVNDNDVFLAGAGKAAIYAFNVDFPLFI
                     QKLAENEQVKVIQSKIIYEIIDDIQNKILHLLEPTIDETVLGEGEIIAEFKIEKIRIA
                     GVKVTKGELTKGDLVHVKRADGITKDTKIDSLRQGKIAVDKVKAGNECGLTFKPYVDF
                     KVKDGILAFTR"
     gene            2861..3670
                     /locus_tag="PK131_01580"
     CDS             2861..3670
                     /locus_tag="PK131_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44852.1"
                     /translation="MKTYNISSIKVLLDGAKSAFIAVPELSLDSVSSALALALSLKDT
                     GLSVQVFCPQKPDANYSKLSGLELLSNVYSANGLVITLDYPQESIETVAYNEDGGRLN
                     LVVKTKANSPKIERDQIIINNQSSLADINFMLGDETKLGKAADIVNRGQWVLISSMPM
                     EKSWAQASIIDPDAPFAEIMAFLIPMLALPFKFEAAKNLLIGLRVATQSFSVNVSPET
                     FEAGAACLRATQVENPSAGELNPFDNVTPLEKIESKGDLFGNLQPSSVSTV"
BASE COUNT         1111 a          693 c          907 g         1134 t
ORIGIN      
        1 agtacggtta agcaggtgat taccaccggt cttaatccgg ctgacaatta ttggaccgaa
       61 gccgaatggc agcgattttg gttcggtagt ttggaggatg aaatcaattt attacggact
      121 ttaccccagc ctttgtatct ttaccattct gatcgtcagc ccgatctgcc ttttgttaac
      181 gactctaagt gttttgagaa agtcagttgg cgggtgtatc gatttaagtg taagtaaatt
      241 tccaatgtca attggaatct ctctgtccgc caaaggcgga ttaacaccac tgcttaatat
      301 cccccctttt gtcaaagggg gattagaggg ggatttaatt tgaattgtga tttttcccca
      361 aggctttttc atgctataat tacccaaagt taagtttagt ttaaattaag ctcaaagaga
      421 tggggatagt gggtccccat tttttttaga aaaaattatg gatcttaaaa ggttacctaa
      481 aaccgaattt gcccaagcgg tggctcaaat tgccggggag cggaatattg atccccagtt
      541 gattttagac tcaattgaag ccggtttgat ttctgcctac aaacgaaatc agagggagca
      601 tggtatcgtt attcccgacg aggatcagtt tgaggttgat ttatcgccgg aatcgggttt
      661 atttaaagtt tttaaaattg aaggagacaa aagacttgag gtaacaccgc ctgattttgg
      721 ccgaattgcc gcccaaactg ctaaacaggt tattgaccaa aaaatttatg aggcggaaaa
      781 agaggtaatt attgctgagt ttcagaaaaa agtagggagt ttggtaacag gggtggtttt
      841 ccgatctgat cccagtaaac tggtaattgg cgttgggaag acagaagcaa tttgtcctaa
      901 agacgaacaa attaaagggg aaaatcttcc cttaggagct aagaaattat ttttgatcaa
      961 agaaattttt cgggatgaaa tcactggtca gagtgaggtg atcctttccc gccgagataa
     1021 caatttcatt aaggaactat ttgcccgaga agtgccggaa gtaagtaacc gggtcgttag
     1081 ggttgaaaag attgctcgga cacccggaga gcggactaaa gtagcggtgg cttccagtca
     1141 acccggtgtt gatccggttg gctcttgcat cggtcaaaaa ggcagccgga ttcaatcggt
     1201 tttaaatgaa ctgccggcag aagaaaaagt tgatgtaatt cttttctcta aaaatttaaa
     1261 tcaatttatt attgctgccc tttctcctgc caatggtgtt cgggttgaag ctattgagaa
     1321 cggcatagct caggttacgg tgccggagga tcagttagct ctagctattg gcggcggcgg
     1381 cgaaaatgtc cgtttagccg gcgttttgac cggtttggaa atcaaaatcg ccgctagaaa
     1441 agagacccaa agttaattag aaattataaa tttattttat gtttcgtcca ccaattgtta
     1501 ctattatggg tcatatcgac cacggcaaaa ctactttgct ggataagatc agatcgacca
     1561 atgtttgtgc taaggaaact ggcggtatta ctcagcatat caatgcttac caagccaagg
     1621 gaattacctt tattgatact cccggccatg ccgcttttat caatatgcgg gcaagaggag
     1681 cgaaaattac cgatatagtt gttttggtta ttgccggtac tgagggagtg atggctcaaa
     1741 ccaaagagtg cctgaagttg gttaaagaat ttaaattgcc cttgatagtc gctcttaata
     1801 agaaagattt ggccggtttt gcccctgata aagttaaagg tcaattgatt gaagccggtt
     1861 ttactcccga ggaatatggt ggtgatgttc cctgtctggc tatttctgcc aaaaccggtg
     1921 agggtatcga taaattactg gaaactatta aattactggc tgatttactt gatcttaagg
     1981 cagaaccaga agctccttta ttggcggccg tgattgaatc ctgtttggat aaaaatcgag
     2041 ggccgatagc cacagcggtg gttgagaaag gaacagtcaa gacaggcgat attatttatg
     2101 ccggttctat tagcggcaaa gttcgagctt taattgatga taacgggaaa aagattgatt
     2161 cggcagggcc atcaacacca gtagtgattc tgggctgtga agcagtgccg ccggtaggtt
     2221 cggtaattac cgaacaaccc caacaggttg cgactgtccc tgcggccaaa ttgactgctg
     2281 attctaccgc ttccaataaa ttaaaaatta ttcttaaggc tgacactcag ggaaccctag
     2341 aggcaattag aaactgttta actgacgacg tcttgatttt ggaagccgga gtcggtgccg
     2401 taaacgataa tgatgttttt ttggctggtg ccggtaaggc cgctatttat gctttcaatg
     2461 ttgattttcc cttatttatc caaaaattag ccgaaaatga gcaagtaaaa gtgattcagt
     2521 caaagattat ctatgaaatt attgacgata tccaaaataa aattcttcat ttgcttgaac
     2581 caacgattga cgagacagtt ttgggagaag gtgaaattat tgccgaattt aaaattgaaa
     2641 agattcggat cgccggggtg aaagtaacta aaggggagct aactaaaggt gatttggttc
     2701 atgttaaaag agccgacggt attactaaag acaccaaaat agatagtctt cgacagggaa
     2761 aaatagccgt agataaagtt aaggccggta acgaatgcgg tttaactttt aaaccttatg
     2821 ttgatttcaa agtaaaagat ggtatactag cgttcactcg atgaaaactt ataatatttc
     2881 atcaattaag gttttacttg acggtgccaa aagtgctttt atcgccgtgc cggaattgag
     2941 tttggacagt gttagttctg ccctagcttt ggcgttatcg ttaaaagata ctggcttgtc
     3001 tgttcaggtt ttttgtcctc aaaaacctga tgctaattat tccaaattaa gtggtttgga
     3061 gttgttaagt aatgtttata gtgccaacgg tttagtgatt accttagatt atcctcagga
     3121 aagcattgaa acagtggctt ataacgagga tggcggccgg ctgaatttag ttgtcaaaac
     3181 taaagctaac tcccctaaaa ttgagcgtga tcagattata attaataatc aatcatcttt
     3241 ggcggatatt aattttatgc tgggtgatga aaccaagttg ggtaaggcgg ctgatattgt
     3301 taatcgtggc caatgggttt taattagttc tatgccaatg gaaaaatcat gggcgcaggc
     3361 gagtattatt gatcccgatg ctccttttgc tgaaattatg gcgtttttaa ttccgatgct
     3421 agctctgccc ttcaaatttg aagcggccaa aaacctgtta attggtctac gggtagccac
     3481 ccaatctttt tcagtaaatg tttctccgga aacctttgaa gctggggctg cctgcttacg
     3541 ggcgactcaa gtggaaaatc cgtcggcggg tgagttgaat cctttcgata atgtaactcc
     3601 cttagaaaaa attgagtcta aaggcgattt atttgggaac ctccagccaa gttccgtttc
     3661 aactgtatga aagaaaaaat tttgcgttgc ggccaatgtt caaaattcac gccatggctg
     3721 agttttgatc atcccagtca atatcaagag gataaggaaa cctttttaag attgcttcgg
     3781 aatccagccg tgccagccga agttaaaatt agagttgttt tggagtcctg cggcaattgt
     3841 cagac
//
LOCUS       DAOOIE010000040         3829 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_18470_length_3829_cov_3.202703, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000040 DAOOIE010000000
VERSION     DAOOIE010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18470_length_3829_cov_3.202703"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PK131_01585"
     CDS             complement(<1..369)
                     /locus_tag="PK131_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44853.1"
                     /translation="MKTTAQLIKIAFLSLVVGCLLKFPVEIKAQSALGLSAIPPRLEV
                     TIKPGEYVTKEIKIRNESSVERIINTEVVDFIVADDKGTPVQLKDVANDNRWAASSWI
                     HVGRQTKIKPGETKSVMVTIV"
     gene            complement(372..1877)
                     /locus_tag="PK131_01590"
     CDS             complement(372..1877)
                     /locus_tag="PK131_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44854.1"
                     /translation="MKKYSLILIGGVLGVSFFLNLPKRFQVESIPSTNVKDTLSSSQL
                     SYFARVGTGTTAGDSIVRINTAANPSRTTNNLFEGDVVCIGNSGTPGCTQYTVQDIGN
                     TAYFALATTLAPANAFITNYIVATRSAQHVVNFTPQTSVVGGKWQFLIRATSGAAEQA
                     SDGMPDQNGFDIGSNVGAQTTGPGARLRPQDVICPFGIGTSATGIGSTVGIVAANGTT
                     YYYHTITCSLDAGAGNPIGVQASMIIGGVSVTNSTQLINPSPNHTVANEGVADLYNFF
                     IRHLTSGDVVIDGDTARGQLAVVESVRVTATVDPTISFIIDNVGVTNVGTSVCAGATI
                     GANAAAVTATQVPFGSLKLQEFNDLAQRVSATTNAASGYAVTVYESDVLTMVGGSITI
                     PDTTCDSATVCTTASENTWASATQTPSKFGYSLTAVPGFTPNTMTFTTGTTNKYRPFP
                     IGAANAVRIFNNPSTPTAWERSYVCYRLTVTNVQPAGNYENRLVYTATATF"
     gene            2004..>3829
                     /gene="ppdK"
                     /locus_tag="PK131_01595"
     CDS             2004..>3829
                     /gene="ppdK"
                     /locus_tag="PK131_01595"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPD44855.1"
                     /translation="MKKWVYGFTQGQAEGKAAMKDVLGGKGANLAEMVNLGIPVPPGF
                     TISTEVCRYYYENNRKYPEGLKEQIVKALKELERNTGKVFGDPKNPLLVSVRSGAAVS
                     MPGMMDTILNLGLNEKVLAGLVAKNNNLRFGWDSYRRFIQMYANVVENIESEKFEHII
                     KEIKADMGKKDDAELTAEDWQKVVAGYKKVFKLEKGRDFPYDPQEQLSKAIEAVINSW
                     NNPRAIIYRQLNKIDEAKIWGTAVNIQAMDFGNLGDDSGTGVAFTRNPATGEKKPYGE
                     YLMNAQGEDVVAGIRTPKRLESLAEDNEIAYEQLMTIFDRLEKHYRDMQDVEFTIENG
                     RLFILQTRNGKRTAAASVKIAVDLVKEKLVDEKEALLRVEAEKLDQLLHPTLDQQKKG
                     SFRMITKGLPASPGAACGQVVFEAETAVKWVENGNKVILVREETSPEDIAGMNVALGI
                     LTARGGMTSHAAVVARGMGKCCVSGCESMIVSSVNKLFKIGDLTIKEGDLVTLDGSTG
                     EVFLGQVPMVEPTLSDDFKVLMKWANKYRRLGVRSNADTPRDAKVARGFGAKGIGLCR
                     TEHMFFEEDRIFAVRQMILSETLAQRETALAKIFPMQKDDFK"
BASE COUNT         1187 a          684 c          921 g         1037 t
ORIGIN      
        1 aacaatagtt accataactg atttggtttc tcctggttta attttggttt ggcggccgac
       61 gtgaatccac gaactggcag cccagcgatt atcattagcc acatctttta attgaaccgg
      121 agtgccttta tcatcagcaa caatgaaatc gaccacttca gtattgatta ttctttcaac
      181 gctagattca ttccggattt taatttcttt agtcacatat tcgccgggtt taatcgtaac
      241 ttcaagccgg ggaggaatag cactcaaacc aagagcactt tgagctttaa tttcaactgg
      301 aaatttcaat aaacaaccga ccactaggct aagaaaagca attttgatta gttgggcagt
      361 agttttcata gttaaaaggt agctgtagcc gtgtagacta atctattttc atagttgcca
      421 gccggctgga cgttagtaac agtgagacgg tagcaaacgt aagacctctc ccaagcggta
      481 ggagtggaag ggttattaaa aattctgacg gcattagcag ctccaatagg aaatggtcga
      541 tatttgttgg tggtaccggt agtaaaggtc atagtgtttg gcgtaaaacc cggaactgct
      601 gttaaagaat aaccaaactt agagggtgtt tgggtagcac tagcccaagt attttcactg
      661 gcagtagtac aaacggtagc actgtcacag gtagtgtcag ggatggttat tgaaccacca
      721 accatggtta agacgtcaga ttcatagacc gtaacagcat aaccactggc ggcattagtt
      781 gtcgctgaga ctctctgggc taaatcatta aattcctgaa gctttaaaga gccaaagggt
      841 acttgggtag ccgtaactgc ggcggcatta gccccaatag ttgctccggc acacacagaa
      901 gttccaacat tggtaacacc gacattgtca ataatgaaag aaatagttgg atcgacagta
      961 gctgttactc gaacggattc aaccacagct aattgacctc tggcagtatc tccatcaata
     1021 accacatcac ccgaagttaa atgacggata aagaaattat aaaggtcagc tacgccctcg
     1081 ttagccacag tatggttagg agaaggatta atcaattgag tagaattagt tacagaaacg
     1141 ccgccaataa tcattgacgc ctgcaccccg ataggattac ctgctccagc atctaagcta
     1201 caggtaatag tgtgatagta ataggtagtg ccattagcag ctacaattcc cacggtagaa
     1261 ccaattcctg tggcgcttgt tccaatacca aaggggcaaa tcacatcctg cgggcgaaga
     1321 cgagcccccg gtccagttgt ttgagctcca acattactgc cgatatcaaa tccattttga
     1381 tcaggcatcc catcactagc ctgttcggct gctccagaag tcgcccggat taaaaactgc
     1441 catttacctc cgactactga ggtctgaggc gtgaagttaa ccacgtgttg agccgaacga
     1501 gtagctacaa tgtaattagt aataaaagca ttagcaggtg ccaaagtggt ggcaagagca
     1561 aaataggcag tgttaccaat atcttgaacc gtatattggg tgcaacccgg agttccacta
     1621 ttaccgatac aaactacatc accttcgaaa agattattag ttgttcggga aggattagct
     1681 gccgtattaa tcctgacgat agaatcacca gcagttgttc ctgtccctac ccgagcgaag
     1741 taagataatt gggaggaaga aagggtatcc ttaacattag tagatggaat tgattcgact
     1801 tggaaccttt tggggagatt aaggaagaaa gatactccca aaaccccacc aatcaaaatt
     1861 aaactatact ttttcacttt ttttaaaaag actttataat tcaatctaaa aaaagaaact
     1921 attatttgtc aagtagttgt tttaatcatg ttctaaaatt agtcctttgt caagagggaa
     1981 tgattgtgat aaattatctg gatatgaaaa agtgggttta tggttttact caaggtcagg
     2041 cagaaggtaa agcggccatg aaggatgttc taggtgggaa aggagctaat ttagctgaaa
     2101 tggtaaattt agggattccg gttccgccgg gctttactat ttctaccgaa gtttgccgat
     2161 attattatga gaataatagg aaatatcctg aaggattgaa agaacagatt gtaaaagctt
     2221 taaaagagtt ggaaagaaat acgggcaaag tatttggcga ccccaagaat ccactgttag
     2281 tgtcggttcg atcaggagca gcggtttcaa tgccagggat gatggatact attcttaatc
     2341 taggtttaaa tgagaaagtt ttagctgggc tggtagctaa aaataataat ttacggtttg
     2401 gctgggattc ctatcgccgg tttatccaaa tgtatgctaa tgtggttgaa aacattgagt
     2461 cagaaaagtt cgaacatata attaaggaaa taaaggctga tatgggaaaa aaagatgatg
     2521 ctgagttaac tgccgaggac tggcaaaaag tagtggccgg ctataagaag gtatttaaat
     2581 tggaaaaggg aagagatttc ccttatgacc ctcaagaaca gctttctaag gcgatagaag
     2641 ccgtcattaa ttcctggaat aatccccggg caataattta tcgtcagtta aacaaaattg
     2701 acgaggcgaa aatatggggt accgctgtta atattcaagc gatggatttt ggtaatctag
     2761 gggatgattc tggtaccgga gtggcattta cccgtaatcc ggctacgggg gagaagaaac
     2821 cgtatgggga atatttgatg aatgcccaag gcgaagatgt ggtagcaggg attagaacac
     2881 ctaaacggct tgaatcttta gcagaggata atgaaattgc ttatgaacag ttaatgacta
     2941 tttttgatcg gttggaaaaa cattatcggg atatgcaaga cgtagagttt actattgaga
     3001 atgggaggtt gtttatttta cagaccagaa atggcaaaag aactgctgcc gctagtgtta
     3061 agatagcggt tgatttggtg aaagaaaaat tagttgacga gaaggaagct cttttacggg
     3121 ttgaagctga aaaattagat cagcttcttc atcctacttt agaccagcaa aaaaaaggaa
     3181 gttttagaat gataaccaag gggttaccgg ccagtcccgg agcggcctgc ggtcaagtgg
     3241 tttttgaagc tgaaacagcc gtaaaatggg ttgaaaatgg caacaaggta attttagttc
     3301 gggaagaaac cagtccggag gatattgctg ggatgaatgt tgctttggga attttaacag
     3361 cccggggcgg aatgacctcc catgcggcgg tagtagccag aggtatgggt aagtgttgtg
     3421 tttccggttg tgaatcaatg attgtttctt cggtaaacaa attatttaaa ataggtgatt
     3481 tgacgattaa agagggtgat ttagttactc tggatggatc aaccggcgag gtgtttttgg
     3541 gtcaagtacc aatggttgag ccgactttat cagatgattt taaagtttta atgaaatggg
     3601 ctaataagta tcgccggttg ggagtaaggt ctaatgctga tactccccga gatgccaaag
     3661 tagcgagggg attcggggca aaaggtattg gtctctgccg gactgaacat atgttttttg
     3721 aagaagatag aatttttgct gtccgacaaa tgattctttc ggagacgtta gcacaacgag
     3781 aaacagcctt ggccaagatt tttccgatgc aaaaggacga ttttaagca
//
LOCUS       DAOOIE010000041         3822 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_18505_length_3822_cov_3.018582, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000041 DAOOIE010000000
VERSION     DAOOIE010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18505_length_3822_cov_3.018582"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /gene="rplU"
                     /locus_tag="PK131_01600"
     CDS             <1..215
                     /gene="rplU"
                     /locus_tag="PK131_01600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPD44856.1"
                     /translation="SSDQILLTVDNDRVKIGCPIVKEAKVDFEILKNYQGPKITTFRY
                     KGKSRYRKTRGFRPQLTDLKITKITN"
     gene            256..504
                     /locus_tag="PK131_01605"
     CDS             256..504
                     /locus_tag="PK131_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019645663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPD44857.1"
                     /translation="MSHVKAASKANQKTDQAGKRRGVKVFGGQTINLGQIIIRQVGTK
                     FHPGRGTDIGRDFTIFATRSGKVKYFKKQGKNFVAVVD"
     gene            545..1381
                     /locus_tag="PK131_01610"
     CDS             545..1381
                     /locus_tag="PK131_01610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPD44858.1"
                     /translation="MKSVQYIAVDQLEPNPMQPRGPIDSESIGELMESIREHGILEPL
                     IVAQTPAGYQIIAGECRWRAAKALKLKSVPVIIKETTPRQMLEMSIVENLQRIDLNPI
                     ERAKAFLRLKEEFGFDHHQIAAKVGKSVPYVINELKLLNLPDALKDGLLSGLITEGHA
                     RALISIGDTRLMIEAYKIVLKEKASVRRTEDIARRMRKKIEEGKIPITKENLPYFLKS
                     KLDKVSNRLESFFKDQTSKVKIIQSNAQTRVIFQFKGSLDDRSEKLKKLYRAILKTDP
                     WY"
     gene            complement(1358..1945)
                     /gene="lepB"
                     /locus_tag="PK131_01615"
     CDS             complement(1358..1945)
                     /gene="lepB"
                     /locus_tag="PK131_01615"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPD44859.1"
                     /translation="MIHAWEKQKGSIGTIILDFIQSIVLILSIFVLIYMFVAQPNEVK
                     GMSMYPSFNDKDYLLTDKLTYRFSSPQRGDVVVFKAPATEQCAENECEYIKRIIGIPG
                     DRVKIQGNKVYINGELFEEKYLPPEVITNPGQFYKEGIEIVVPPDYYLCLGDNRLHSR
                     DGRDFGPIARDTIVGKAFLRYWPPKSFGLVPRISF"
     gene            2015..2091
                     /locus_tag="PK131_01620"
     tRNA            2015..2091
                     /locus_tag="PK131_01620"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2049..2051,aa:Val,seq:cac)
     gene            2095..2168
                     /locus_tag="PK131_01625"
     tRNA            2095..2168
                     /locus_tag="PK131_01625"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2129..2131,aa:Lys,seq:ctt)
     gene            2289..2963
                     /locus_tag="PK131_01630"
     CDS             2289..2963
                     /locus_tag="PK131_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44860.1"
                     /translation="MAISLIIISRKRIPLPGWVNQCQAINEILIIDDQQFPVDGDFAA
                     KRNYGLKIARNNWCLFLDTDEEPSTQLIEFLNHFNYQQNIAFRRLDLFLGRRLKFGET
                     GHFYSIRLVNRRSGQFVGRVHEIFQTTNYRRVNIPIYHRPHSSFYSFIEKINFYSDLR
                     AKELFDQKRKTNLFWIIFYPLAKFIQNYFLKLGFLDGIVGLIHALGMSFYSFLVRSKL
                     WSMSRP"
     gene            2942..>3822
                     /locus_tag="PK131_01635"
     CDS             2942..>3822
                     /locus_tag="PK131_01635"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPD44861.1"
                     /translation="MEYVAAIIFFFGKLFHPWPVFEIALGLLTLLNLRNFKKINNPYF
                     LIFIIYTLITGRFYNYSSFLYWLRLTCLLSFFVIPPVFSQKYKHFFLLIVSANLIFGL
                     IQYFIWPNFTYFNALNWDPHLFRLISTFLDPTFTGLLYLMFLIVLFCHKLPFYYLILP
                     YVTMSLTYSRSSLLAFLASFAYLAKKLNKPKIFYISSLLLLITLVALPRREGEGTKLE
                     RLSSVRAKIENYQEGITLFKQHPILGVGYNNLKHVRNLDNNLSHSSSGFDGSLLTILT
                     TTGLIGFSFFILGWYHY"
BASE COUNT         1181 a          787 c          629 g         1225 t
ORIGIN      
        1 catcctctga tcaaatctta ttgacggtag ataatgaccg ggtaaaaatt ggctgtccaa
       61 tagttaaaga agctaaggtt gattttgaaa ttcttaaaaa ttaccaggga cccaaaataa
      121 ctacctttcg ctataaaggt aaatcacgtt atcgtaaaac tcgcggtttt cgacctcaat
      181 taaccgactt gaaaataact aaaattacaa attaaaaatt agaaaaataa ttagaaatta
      241 gaaattgtct aaagtatgtc tcatgttaag gcagccagta aggctaatca aaaaacagat
      301 caggccggca aaagacgtgg ggtaaaagtc tttggtggtc aaaccattaa tcttggtcaa
      361 ataatcatcc gacaggtggg tactaaattt catcccggcc gaggcactga cattggtcga
      421 gatttcacca tctttgccac tcgttctgga aaagtcaaat acttcaaaaa gcaaggtaaa
      481 aattttgtgg ctgtagttga ctaggggctc tttttcactg ccgttttcat ggtaaaatta
      541 aattatgaaa tcggttcaat atattgccgt tgaccagtta gaacccaatc ctatgcaacc
      601 ccgtggtcca attgattctg aatcgattgg tgaattaatg gagtctattc gtgaacatgg
      661 tattcttgaa ccactaattg tcgctcaaac cccagccggc tatcaaatca ttgccggcga
      721 atgccgctgg cgggccgcaa aagctctcaa attaaaatcc gttccagtaa ttattaagga
      781 aactactccc cggcaaatgt tggaaatgtc cattgttgaa aatctccaaa gaattgattt
      841 aaatccaatt gaacgagcta aggcttttct acggctaaaa gaggaattcg gttttgatca
      901 tcatcaaatt gccgctaagg taggcaaaag tgttccttat gtcattaatg aattaaaatt
      961 gctcaacctg cctgacgccc tcaaagacgg tcttctttct ggcctaatta ctgaaggcca
     1021 tgcccgagcc ttaatttcaa tcggtgacac ccgactaatg attgaagctt acaaaattgt
     1081 tctcaaagaa aaagcttcgg tccgacgaac tgaagatatt gctcgacgaa tgcgaaaaaa
     1141 aatagaagag ggtaaaatcc ctattactaa ggaaaatctg ccttatttcc tcaaaagtaa
     1201 attagacaaa gtcagcaacc gactagaaag cttttttaaa gaccaaactt ctaaagttaa
     1261 aatcattcaa agtaatgccc aaaccagagt tatttttcaa tttaaaggat cccttgatga
     1321 tcgatctgaa aaacttaaaa aactctatcg ggctatttta aaaactgatc cgtggtacta
     1381 aaccaaacga ttttggcggc caataacgta aaaaggcttt gccgacaata gtgtctctgg
     1441 caatcggtcc aaaatcacga ccatcgcggg aatgtaaccg gttatctccc aagcataaat
     1501 aataatcagg tggaactaca atctctatcc cttctttata aaactgacca gggttagtaa
     1561 taacttccgg cggtaaatat ttttcctcaa acaactcacc attaatatag actttattcc
     1621 cttggatttt tacccgatcg ccaggaatac cgataattct cttaatatat tcacattcat
     1681 tctcggcaca ttgttccgtt gctggcgctt tgaaaacaac cacatcgccc cgttgaggcg
     1741 aactaaatct ataagtcaac ttatcagtca ataaataatc cttatcatta aaacttggat
     1801 acattgacat tcccttaact tcatttggct gggcaacaaa catataaatt aaaacgaaaa
     1861 ttgatagaat caaaacaatc gattggataa aatccaaaat aatggtgcca atactaccct
     1921 tttgtttttc ccaagcatga atcatagatt taagtttaac cactcccttt gctataatca
     1981 actgtcagtt taattcaaaa atagaaatta aactggttgc ttagctcagt tggttagagc
     2041 gcttcgttca catcgaagag gtcaggggtt caaatcctct agcaaccacc acaagggctg
     2101 ctagctcagt ttggtagagc aatcgcctct taagcgatcg gtcgtgggtt cgagtcccac
     2161 gcagctcact aaggcttgac tttttacaaa tcccaattta acaacaataa attggcttat
     2221 caagtagcag tctaattaaa aaaatggggg taaaatacaa agaaacggtc ataatgttaa
     2281 aatagatcat ggctatttct ctgattatta tcagccgaaa acggatccct ttgccggggt
     2341 gggttaatca atgccaagca attaacgaaa ttcttattat tgatgatcaa caatttccag
     2401 ttgatggtga ttttgccgct aaacgcaatt atggcttaaa aattgctaga aataattggt
     2461 gtctttttct cgataccgac gaagaaccat caactcaact aattgaattt cttaatcatt
     2521 ttaattatca acaaaatatt gccttcagac gactggatct tttccttggc cgtcgtttaa
     2581 agttcggaga aaccggccat ttttactcta ttcgtttagt taaccgtcga agtggccaat
     2641 ttgttggccg ggttcacgag atttttcaaa caaccaacta ccgtcgggtc aacataccaa
     2701 tttatcatcg accccattct tctttttatt cttttatcga aaaaatcaat ttttacagtg
     2761 accttcgggc caaggaactc tttgaccaaa aaagaaaaac caatttattt tggattattt
     2821 tttatccgct agccaaattc attcaaaatt actttttgaa acttggtttt cttgacggta
     2881 ttgtcggttt aatccatgcc ctaggaatga gcttttattc atttttggtt agatcaaaac
     2941 tatggagtat gtcgcggcca taattttctt cttcggtaaa ttatttcatc cttggcctgt
     3001 ttttgagatt gccctcggcc tgctgactct tcttaattta agaaatttta agaaaattaa
     3061 caatccctat tttttaattt ttataattta taccctaatt accggcagat tttacaatta
     3121 ctcctcattt ctttactggc ttcgtcttac ctgtttactt tcattttttg ttattccgcc
     3181 ggtttttagc caaaaatata aacacttttt tttattgatt gtctcggcca atcttatttt
     3241 tggtctcatc caatatttta tctggccgaa ttttacctat tttaacgctt taaactggga
     3301 tccgcattta tttcgactca tctccacttt tcttgatcca acttttaccg gcttacttta
     3361 cctcatgttc cttattgttc ttttctgtca taaattaccg ttttattatc taatcctgcc
     3421 ttatgtgaca atgagtttaa cttacagccg ttctagcctg ctggcttttt tagcctcttt
     3481 tgcttattta gccaaaaaac tcaataagcc aaaaattttt tatattagca gtctgttatt
     3541 attaattacc ctggttgcct taccccgccg agaaggggag ggaaccaaac tagaacgtct
     3601 ctccagcgtc agagcaaaaa tagaaaatta tcaggaggga ataactcttt tcaaacaaca
     3661 tcctatcctt ggcgttggct ataacaactt aaaacacgtc cgtaatttag ataacaatct
     3721 ttcacattct tcctccggat tcgatggtag tttattaact attctcacca ccaccggttt
     3781 aattggtttt tcttttttta ttctaggttg gtatcattac tt
//
LOCUS       DAOOIE010000042         3786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_18763_length_3786_cov_2.905119, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000042 DAOOIE010000000
VERSION     DAOOIE010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18763_length_3786_cov_2.905119"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PK131_01640"
     CDS             <1..507
                     /locus_tag="PK131_01640"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HPD44862.1"
                     /translation="DDCHFKYELVTSRTYEIAAKLAKINPRFHQYLFEIDNNNSPEKI
                     KFLGIALSKVETFFNNQAAISFVPFEIMDQNRFPKSATENTDVANILKSVINWQIGGT
                     FFEYQQDKIKVGFRTRDARKYDLTKIAKALGGGGHRAAAGANLEMPFDQAKKLVIVQL
                     QRIYFSNC"
     gene            534..839
                     /locus_tag="PK131_01645"
     CDS             534..839
                     /locus_tag="PK131_01645"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HPD44863.1"
                     /translation="MTDCTFCRIIKGELPATKVYEDENYLAFLDIQPQAEGHTLLIPK
                     THYATVYDVPNFGQYFEKARDVANLLKEKYQADYIFLKVIGTDVPHAHIHLIPYKSS"
     gene            847..1230
                     /locus_tag="PK131_01650"
     CDS             847..1230
                     /locus_tag="PK131_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44864.1"
                     /translation="MNQTTRAGLKRWSLFLIIAVVVGGFCFAAQPPKNQIIFYYGEEC
                     PHCHNVLKFIADNQIDQKIKIETKEVYHQTQNQRELAALAKICPEIIDSSGNIGVPVA
                     LITREKKCLQGDTAIIEKIKTIMLQ"
     gene            1234..1557
                     /locus_tag="PK131_01655"
     CDS             1234..1557
                     /locus_tag="PK131_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44865.1"
                     /translation="MGKSHRQFKLTWAVIFRLTLFLVIIISAISYIAGLIGQDRILRY
                     NWNLTSLYLKLPETTRDRLADLGQKAEVIKQESINFIDRQIKEIEKRMVEQISGRVIE
                     MIEKQ"
     gene            1554..2564
                     /gene="prfB"
                     /locus_tag="PK131_01660"
     CDS             1554..2564
                     /gene="prfB"
                     /locus_tag="PK131_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_161931791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPD44866.1"
                     /translation="MKEKLAQLKKKLGFNEKQFELEKLKKSAEDPNLWTNPNQAQKLL
                     QEIANLEKLINSIKEIEESLEYVEAATPAEITELENKLNKLELETYLSGKYDKNNCIF
                     SIHAGQGGTEAMDWAAILQRMYLKYFERKNWKYQVLDLVPGEETGIKSCDFKVVGLYA
                     YGYLKGENGVHRLVRLSPFNADQLRQTSFAKVEVMPIIDNPQEVNLNPDDLEFAAFRS
                     GGHGGQNVNKVSTAVRLTHRPTGITVSCQSQRFQEQNRAVCLEMLKSKLWMLEQEKLN
                     QEKRAIKGENVIAGWGHQIRSYVLHPYKMVKDLRTQVETADPARVLDGDLDLFIEAEL
                     KL"
     gene            2621..3541
                     /locus_tag="PK131_01665"
     CDS             2621..3541
                     /locus_tag="PK131_01665"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HPD44867.1"
                     /translation="MNKIVKLIVLLLVCLITIFYFTNKNNPLNTQNNFNIIILGLDPR
                     NDTLEKTETTDTIIFASFNLRRAELNLISLPRDLWNYATNSKINQIYPDNIDDFGKIQ
                     LEFASITGQTIHRTIIITTQNLIDLVDLIGGVDVYLEQGFKDEKYPNPDYIKNPSPKI
                     SPYKTVEFPSGWIHLDKTNVAEFVRSRHADSDLARIQRQQLLIEAIFKKMTQPQYLSK
                     LNYFYRHNLKTNLNYNDIVNLILNLKFKILNPTSQAGLKLNKIDLPIGSKAGEGVIYY
                     PNKLTRNQWAFLPSDPDYQALKEFIDKAIK"
     gene            3574..>3786
                     /locus_tag="PK131_01670"
     CDS             3574..>3786
                     /locus_tag="PK131_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44868.1"
                     /translation="MEIQNLTSNPLSTAPIKKTPILSWPGLIIVIILGITSGFWLSRL
                     KGNSTSLDNHSLLSSEKTTSVEEIGKI"
BASE COUNT         1261 a          721 c          699 g         1105 t
ORIGIN      
        1 gacgattgcc atttcaaata cgagctggtg accagcagaa cctatgaaat tgccgctaaa
       61 ttggcaaaaa taaatccccg cttccatcag tacttgtttg aaatcgacaa taacaatagt
      121 ccggaaaaaa tcaaattttt ggggatagct ctgtctaagg tagaaacttt ctttaataac
      181 caagcggcta tttctttcgt tccttttgaa attatggacc aaaatcgctt tcctaaaagt
      241 gcgactgaaa ataccgacgt cgctaacatt ttaaaatcag ttattaattg gcagatcggc
      301 ggcacttttt ttgaatatca acaagataag attaaggttg gcttccgcac tcgagatgcc
      361 aggaagtacg atttaaccaa gattgccaaa gctttgggcg gtggtggaca tcgagccgcc
      421 gccggcgcca atttagaaat gcctttcgat caggccaaaa aactggtaat agttcaactt
      481 caaaggattt actttagtaa ttgctagttg ctaactatgt tattatctaa actatgactg
      541 attgtacctt ctgccgaatt attaaagggg agcttcctgc tactaaagtt tatgaagatg
      601 aaaattatct ggccttttta gatatccagc ctcaagccga gggccacacc cttttgatcc
      661 ccaaaaccca ttatgcaact gtttatgatg ttcctaattt cggccaatat tttgaaaaag
      721 cccgtgatgt cgccaattta ttaaaagaaa aatatcaagc cgactatatc ttccttaaag
      781 tcatcgggac cgatgtgcct cacgctcata tccatttgat tccctataaa tcttcttgaa
      841 agtctaatga accaaacaac ccgagccggt cttaaacgat ggagtttgtt tttaataata
      901 gccgttgttg tcggagggtt ttgtttcgcc gctcagccgc caaaaaatca aatcattttt
      961 tattacggcg aggaatgtcc tcactgtcat aatgttttga aatttattgc cgataatcaa
     1021 attgaccaga aaataaaaat tgaaaccaaa gaagtttatc accagaccca aaatcaaaga
     1081 gagttagccg ccttggcaaa aatttgtcca gaaattatcg atagttctgg taatataggt
     1141 gttccagtcg ctttaataac cagagaaaaa aagtgccttc agggcgatac cgctattatc
     1201 gagaagatca aaacaataat gctacaataa tttgtgggta aatcacaccg ccaatttaaa
     1261 cttacgtggg cagttatttt tcgattaact ctttttctgg tgattattat cagcgctatc
     1321 agttatattg ccggactgat tggccaagac cgaattttaa ggtataattg gaacttaacc
     1381 tcgctttatt tgaaactgcc ggaaacaacc agggatagac tggcagattt aggccaaaaa
     1441 gcagaagtaa taaaacagga aagtatcaat tttattgatc gtcagattaa agaaattgaa
     1501 aaaagaatgg ttgaacaaat aagcggccgg gttatagaaa tgattgaaaa acaatgaaag
     1561 agaaattagc ccaattaaag aaaaaattgg gttttaacga aaaacaattt gaactggaaa
     1621 agttgaagaa gtcagctgaa gatcctaact tatggacaaa tccgaatcaa gcccaaaaat
     1681 tattacagga gatagctaat ttagaaaagt taatcaattc cattaaagaa attgaagaga
     1741 gtctagaata tgtggaagcg gcaacaccgg cggaaattac cgaattggaa aataagttaa
     1801 ataaattaga attggaaaca tatttatcgg ggaaatacga caaaaataat tgtatttttt
     1861 ccattcatgc cggccagggc ggcactgaag ccatggattg ggcggcaata ctgcaaagaa
     1921 tgtatcttaa atatttcgag cggaaaaact ggaaatatca agtcttagat cttgttcccg
     1981 gcgaagaaac tggtattaaa tcttgtgatt ttaaagtggt tggtctttat gcttatggtt
     2041 atttaaaggg ggaaaatgga gtccatcgtt tagtccgtct ttctcccttt aatgccgatc
     2101 aattgcgtca aacctcgttt gccaaagtcg aagttatgcc aataattgac aatcctcaag
     2161 aagttaatct aaatccggat gacttagaat ttgccgcttt tcgctccggt ggtcatggcg
     2221 gccaaaatgt caataaagtt tcgacagctg tccgactaac tcataggccg accggcatta
     2281 ctgtttcctg tcaaagccag cgatttcagg aacaaaaccg ggcggtgtgt ttggaaatgc
     2341 tcaaaagtaa actttggatg ttggaacagg aaaaactcaa tcaggaaaaa agggccatta
     2401 aaggggaaaa tgtcattgcc ggctgggggc atcaaatccg ctcttatgtc cttcaccctt
     2461 ataaaatggt caaggacttg cggactcagg tagaaactgc cgatccggca agagttttgg
     2521 atggcgattt agatttattt attgaggcgg aattaaaact ttaaatgtca taaagatgcc
     2581 acgcggctgc ggggcatctc tgtaagatag aatgtaaact atgaacaaaa tagtcaaatt
     2641 aatagtctta ctgttggttt gtttaattac tattttttat tttactaata aaaataatcc
     2701 cttaaatacc caaaataatt tcaatataat tattcttggt cttgatcccc gaaacgatac
     2761 cctagaaaaa accgaaacca ccgataccat catttttgcc tcctttaatt tgagaagagc
     2821 cgaattaaat ttgatttctc tgccgcggga tttatggaat tacgccacta attccaaaat
     2881 taatcaaatt tatcccgaca atattgatga ttttggcaaa attcaattgg aatttgctag
     2941 tattaccggc caaactattc atcgaaccat aattattacc actcaaaatc tgattgattt
     3001 ggttgatctc atcggcggag ttgatgtcta tttggaacaa ggtttcaagg atgaaaaata
     3061 tcccaaccct gattatatta aaaacccatc tcccaagatt tctccctata agaccgtcga
     3121 atttccatcg ggttggattc atttagacaa aaccaatgtg gccgaatttg tccgctcccg
     3181 acacgccgac agtgatttag cccggattca acgccaacaa cttcttatcg aagctatttt
     3241 caaaaaaatg actcaaccgc aatatctttc aaaattaaac tatttctacc gtcataacct
     3301 aaaaaccaat ctcaattata acgacatagt taatttaatt ttaaatttaa aattcaaaat
     3361 tttaaacccg actagtcagg cgggcctaaa attaaataaa attgatctgc ctattggttc
     3421 taaagccggt gaaggggtaa tttattaccc taataaatta actcggaatc aatgggcttt
     3481 tctgccatca gaccccgatt atcaggctct gaaagaattt atcgacaaag cgattaagta
     3541 gattgtaaaa aattaccttg ttataattta attatggaaa ttcaaaattt aacttctaac
     3601 cctctgtcca cagcaccaat taaaaaaact cctattcttt cttggccggg attaatcatc
     3661 gttattattt taggaatcac ctccggattc tggctctccc ggctgaaagg aaattcaacc
     3721 agtttagaca atcattctct gttgtcgtca gagaaaacta cttctgtaga agaaatcggt
     3781 aaaatt
//
LOCUS       DAOOIE010000043         3776 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_18831_length_3776_cov_3.135179, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000043 DAOOIE010000000
VERSION     DAOOIE010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3776)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3776
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18831_length_3776_cov_3.135179"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..743
                     /locus_tag="PK131_01675"
     CDS             <1..743
                     /locus_tag="PK131_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPD44869.1"
                     /translation="FDQTQQNAADNVLRSQVAPTYTKTNVDFTKTVDDALRRILNQSQ
                     ITLDNSVINVELANYALQLSTLISPISGIITHEDVSVAGVNVTPATTFVVADPDSIVF
                     RANVSAANIYYVTDGSPATLILDGLQNKLEGTVAKIYPSKVVLNNGQTFYQVDIVSDD
                     LRKLGKLDEMGRAIIKTNSENVALVPAWTVIGGGYIWTNNKGVCELKKVTVGKIHGDQ
                     MEVLSGLNPGDEIIIDPQLISNQQYSYL"
     gene            740..1378
                     /locus_tag="PK131_01680"
     CDS             740..1378
                     /locus_tag="PK131_01680"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPD44870.1"
                     /translation="MKTIIALLKNPKFWKITSLICLLLVIIGSYFYYQKTVDRVFISK
                     SVIQAPVITVSPSLTGKVTEISVKEGQMVETGDKLAVVGSEVLRAETDGLVISANDLT
                     GSTVNQATQLIQMIRPVNMRVVGTLDENKGLNKIKVGQVVSFTIDALPGRIFWGYIDE
                     VSPSALVPMFSFSTSSERTTQQFTVKAKFNTSASPEIKNGMSAKMIVYTRTR"
     gene            1427..3196
                     /locus_tag="PK131_01685"
     CDS             1427..3196
                     /locus_tag="PK131_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPD44871.1"
                     /translation="MLSKEAVEDRNLIDRAYDFAEFHHRGQLRAGGEPYFTHCEAVYR
                     ILKDEFGLNDENILAAALLHDIVEDTTIDLTVLNQEFNPQVAELVDGVTKLSTKTNKD
                     TLIKVLSKTYLNPTVAVIKLADRLHNLRTMDDMPVEKQNAKARETLDVYTRLAESLGL
                     WSIKTEIEDLCFKFLYPQEYLKIKDTISTDFRLDPLFTAFFKSRLEQLLVENNYSGRV
                     EIRKNGYWAVKEKQRKLALEGKCNPQDFKQINDLVSFRVLLPEIDYCYRFLGCLHQKL
                     GQNVDFSKFDEYIGANQRINGYQALQTTLNLTQGAVEVALVTPQMEEFNNWGVVSLIR
                     KGETDLKDYALKIIFTPSNSIRFLPKDATGADFAAAIYPQLLAEAEYILIDGQQAPLS
                     TVLPHTSTVEVITGSFRRAPLVGIEEFCLPSTRKIIQEQRIQENRDQLIAAGKEIMQT
                     ILAPRGLLDLRDLDEKIVKPVINRFGCQSRDDLYFQLGNKALSNLELNQALDAAGITK
                     EKLGLTTVKLIGRNRPKILMDVAAAISNQGQADIIRTEQKQSQAADLFDLKIVVKNLT
                     KSEEDQLRCSFQSDPRFTTVLVV"
     gene            complement(3174..>3776)
                     /locus_tag="PK131_01690"
     CDS             complement(3174..>3776)
                     /locus_tag="PK131_01690"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HPD44872.1"
                     /translation="ISRAGLKQFRVTETEKWIYVTTIFSGMREAPFEGEDRLLIESDK
                     IPTYDLQPKMHTMDIAQAAVKAINSDQYHLIVINFNNPDIIGHTGNIPAAVIGIEECD
                     KGLALLVEAVKTKHGLLIVSADHGNAESMLTSDGQPETQHSANNVPFIIVNDDPQYKN
                     IKLKSGGALKDVTPTILEILGIPQPTEMTGKSLIKPPAQW"
BASE COUNT         1110 a          664 c          809 g         1193 t
ORIGIN      
        1 cctttgatca aacccagcag aatgctgccg ataatgtgct gcgttcgcaa gtagccccaa
       61 cttataccaa aactaatgtt gattttacta agacagtcga cgatgcttta aggcgtattt
      121 tgaatcaaag ccaaattact ctagacaatt cagttattaa tgtggaattg gctaattatg
      181 ccttgcagct gtcaacttta atttcgccga ttagtggcat tattacccat gaagatgtaa
      241 gtgtggccgg ggttaacgtg actccagcta ccacctttgt agttgctgat cccgattcaa
      301 tagtttttag agctaatgtt tcagcagcca atatttacta tgttactgac ggcagtccgg
      361 ccactttaat tttagacggc cttcaaaaca aactagaagg aacagtagct aaaatttatc
      421 cgtcaaaagt ggttttgaat aacggccaaa ctttttatca ggtggatatt gtgagtgatg
      481 atctaagaaa acttggtaaa ttggacgaaa tgggacgagc gattattaag actaattcgg
      541 aaaatgtagc tttggtacca gcttggacag taatcggtgg tggatatatc tggactaaca
      601 ataagggggt ttgtgagtta aagaaggtga ctgtcgggaa aattcacgga gaccaaatgg
      661 aggttctcag cggtttgaat ccgggtgatg agattattat tgatccccag ttaatttcaa
      721 atcaacagta ctcatattta tgaaaacaat aattgctctt ttaaaaaatc ctaagttttg
      781 gaaaataaca agtctaattt gtttattgct agtgattatt ggcagttatt tttattatca
      841 gaaaactgtc gatcgggtct ttatcagtaa atcggttatt caggcaccgg ttattactgt
      901 ttccccgagt ttaaccggca aggttactga aataagtgtt aaagaagggc agatggttga
      961 gactggtgat aaattagctg tggtaggatc agaggtttta agagctgaaa ctgatggttt
     1021 agttatttcg gcgaatgatt tgactggttc aactgttaat caggcaaccc aattgattca
     1081 aatgattagg ccggttaata tgagagttgt aggcaccctc gatgagaata agggtttaaa
     1141 taaaattaag gttggtcagg tagtttcttt taccattgat gctctaccag gtaggatttt
     1201 ttgggggtat attgacgaag tttccccgag tgctttagtt ccgatgtttt ctttttcaac
     1261 ttcgagtgaa cgaactaccc aacaatttac agttaaagct aaatttaata cttctgcttc
     1321 tccagagatt aaaaatggta tgtcggctaa aatgatagtt tatacaagaa ccagatagaa
     1381 attggcctaa gtttgatttt gtcctatagt agctataaaa tacggcatgt tgtctaaaga
     1441 agctgttgaa gatagaaatt taatcgatag ggcttatgat tttgctgaat ttcatcatcg
     1501 aggtcagttg agggctgggg gtgaacctta ttttacccat tgtgaagcgg tttatcggat
     1561 tctaaaagat gaatttggtc ttaatgacga gaatattctc gccgccgctc ttctgcatga
     1621 tatagtggaa gatacaacta ttgatttaac tgttcttaat caggagttta atccacaagt
     1681 agcggaatta gtcgatgggg taactaaatt aagtactaaa actaataagg acaccttaat
     1741 taaagtcttg agtaaaactt atcttaatcc gacagtggcg gttattaaat tagccgatcg
     1801 attacataat ttgcgaacta tggatgatat gccggtcgag aaacaaaatg ccaaggctcg
     1861 agagaccctc gatgtttata cccgtttggc agaatcattg ggtttatggt caattaaaac
     1921 cgaaattgaa gatctgtgct ttaagtttct ttatcctcag gaatatctca aaattaaaga
     1981 tacaattagt accgatttcc gtcttgatcc tttgtttacg gctttcttta aaagtcgttt
     2041 agagcaactt ttggtggaaa ataattattc tggccgggta gaaatcagaa agaatggtta
     2101 ttgggcggtt aaagaaaagc agaggaaatt agccttagaa ggtaaatgta atccccaaga
     2161 ttttaaacaa atcaacgatt tagttagttt tcgggtgctt ttgccagaga ttgattattg
     2221 ttatcgtttt ctgggttgtc tccatcagaa attgggacaa aatgtcgatt ttagtaagtt
     2281 tgatgaatat atcggggcta accaacgaat taacggttat caagccctcc agacaactct
     2341 taatttaacc caaggagcgg ttgaagttgc cctagttacc ccccaaatgg aagaatttaa
     2401 taattgggga gtagttagtt tgatcagaaa aggagagact gatctaaaag actatgcctt
     2461 gaaaattatt tttaccccat ctaacagtat tcgctttctg ccgaaggatg ctaccggggc
     2521 tgattttgcc gctgctattt atccccagct tttagctgag gccgaatata ttttgattga
     2581 cggccaacag gcgcctttgt caactgtttt accccatacc agcactgttg aagtgattac
     2641 tggttccttt cgtcgagctc ctttagttgg tattgaggaa ttttgtttac cttctaccag
     2701 aaagataatt caagaacagc gaattcaaga aaatcgtgac cagttaattg cggctggtaa
     2761 ggaaataatg cagactattt tagctccccg cggccttctt gacttgcgtg atttagatga
     2821 aaaaatagtt aaaccagtca ttaatcgatt tggttgtcag agtcgcgatg atctttattt
     2881 tcagctgggt aataaagctt tgtccaattt ggaattaaat caggctcttg atgccgccgg
     2941 cattacgaaa gaaaagctag gtttaacaac cgtcaaattg attggccgta atcgtccaaa
     3001 gattctaatg gatgtcgctg cagcaattag taatcaaggt caggctgata tcatccggac
     3061 tgaacagaaa caaagccaag cggccgacct gtttgattta aaaatcgtcg ttaaaaattt
     3121 gaccaaatca gaagaagatc aattacgctg ttcttttcaa tctgatcctc gttttaccac
     3181 tgtgctggtg gtttaattag acttttacct gtcatttcag tcggctgagg tattccaaga
     3241 atttctaaaa ttgtcggggt aacatctttc aacgctccgc cggattttaa cttgatgttt
     3301 ttgtattgcg gatcgtcatt aacaataata aagggaacat tgttggccga gtgttgggtt
     3361 tccggctggc catcagaagt tagcattgat tcggcattac cgtgatcagc tgagacaata
     3421 agcagaccat gtttggtttt tactgcttct accaacagag ccaatccttt gtcacattct
     3481 tcgatgccga tgaccgccgc tggaatatta ccggtatgtc caataatatc tgggttgtta
     3541 aaattgatga caattaaatg atattggtcg ctattgatag ctttaaccgc cgcttgggca
     3601 atatccatag tatgcatttt gggctggaga tcataggttg gaattttatc cgattcaatc
     3661 aataaccgat cctcaccttc aaaaggtgcc tctcgcattc ctgaaaaaat ggtagttacg
     3721 taaatccact tttccgtttc ggttactcgg aattgtttta gccccgcccg actgat
//
LOCUS       DAOOIE010000044         3756 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_18957_length_3756_cov_2.467441, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000044 DAOOIE010000000
VERSION     DAOOIE010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18957_length_3756_cov_2.467441"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..487)
                     /locus_tag="PK131_01695"
     CDS             complement(35..487)
                     /locus_tag="PK131_01695"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HPD44873.1"
                     /translation="MNQIYVGADHRGYRLKEIIINQLREGGYSVEDTGNSQYNIEDDF
                     TDFAIKTAEMVIRGGARGILICGSGLGMSIIANKVKGIRAGLCTSIKQARLGRNDLDI
                     NLLCLSADLVSDEKNLKIVQKFLETVFAPEERYLRRIKKITQYESEKC"
     gene            513..1019
                     /locus_tag="PK131_01700"
     CDS             513..1019
                     /locus_tag="PK131_01700"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPD44874.1"
                     /translation="MGQLKTLTILDQSYQLLIFPGYYDSLVIDEDRQQILINTFSLTS
                     AHLGELIDRKMRPFALKKIKLELNKLAAEFGFEYNRLTLRRQSSCFGSCSGRNNLNFN
                     WQIIFLPYPIFRHLLLHELTHLSVKNHKRQFWDQLKFYDPQTEVHHHWLKTNGRRCFL
                     FLDKTALF"
     gene            1035..1445
                     /locus_tag="PK131_01705"
     CDS             1035..1445
                     /locus_tag="PK131_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44875.1"
                     /translation="MIENKDNYLDLFKKTGLDDHIHYQNFRNPWTSREEEIIDVFSPL
                     MRIQVRKKNHQNPIFHEVYIYPDGRLITLPTLKAITDYLEQKQLVSNFGFNEETNTFK
                     AEVNFEGLKYICQVVKVITQPQSDNGQNPEPRVK"
     gene            complement(1384..2145)
                     /locus_tag="PK131_01710"
     CDS             complement(1384..2145)
                     /locus_tag="PK131_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPD44876.1"
                     /translation="MSIRIISWNINGIRAAVKRGLVDFIKKDKADIYCLQEVKASQNQ
                     IDLRLKEYTGYWNSAKKAGYSGVLTLSKLEPKAVIIGDRDSDWDDEGRVIITKFDKFT
                     LLNVYFPNGKKDRGRLMYKLEFYEYFLQYINKLRDQGEKIVFCGDVNTAHREIDLARP
                     KENENISGFLEIERKWLDKLEQNGYIDTYRWLHPQEIGYSWWSPRSGARKRNIGWRID
                     YFFVSDNLKNQIKKAFILPEVQGSDHCPIGVELSL"
     gene            2246..2318
                     /locus_tag="PK131_01715"
     tRNA            2246..2318
                     /locus_tag="PK131_01715"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2279..2281,aa:Tyr,seq:gta)
     gene            2321..3391
                     /gene="recF"
                     /locus_tag="PK131_01720"
     CDS             2321..3391
                     /gene="recF"
                     /locus_tag="PK131_01720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HPD44877.1"
                     /translation="MYLSQIILENFRSYSQKVFEFDPSVNLIIGQNGLGKTNLLEAIY
                     FVATGKSFRALSLRQLIYWGKDMADIRAQIKDSQGGDKELEVQLVTVRQLTTAKTTRK
                     FYLNGVEKTRSHYLGLLSVVAFQPEDIRLIAGSPTRRRHLLDEVLSQLDWHYRVALFQ
                     YEKALKHRNELLDQIRLGKNLKAELFYWDKSLIKNDEIIHHYRGKFIQSANDFFSSHP
                     DAEIKTIFLNFQPSVLSQSVLDKNYQKDLTWGYTSAGNHRDDFSFDNLNFPDQYRNLE
                     YWGSRGEQRLAVLALRLAQIAFYEQTHQEKPILFLDDVFSELDPNHRQLVIKICQHYQ
                     TFFTFTSHDIDPLLPSAKVINL"
     gene            3504..>3756
                     /locus_tag="PK131_01725"
     rRNA            3504..>3756
                     /locus_tag="PK131_01725"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1119 a          700 c          675 g         1262 t
ORIGIN      
        1 aatagccaaa gaaccgaccg caggcaggta ttgttcaaca cttctcagat tcatattgag
       61 tgattttttt aattcttctt aaataacgtt cctccggagc aaaaactgtt tctaaaaatt
      121 tttggactat ttttaaattt ttttcatcag agactaaatc agctgataaa cacaaaagat
      181 taatatctaa gtcatttctg cccagtcttg cctgtttaat tgaggtacat aaccccgctc
      241 gaataccttt gactttattg gcgatgatac tcattcctaa accagaacca caaataagga
      301 taccccttgc ccctccccga attaccattt cagctgtttt aatggcaaaa tcggtaaaat
      361 catcttcaat attatattga ctgttgcctg tatcttcaac actataaccc ccttccctta
      421 attgattaat aataatctct tttaaccgat atccccgatg atccgcacca acataaatct
      481 gattcataaa ttacactata atcatatcat aaatgggaca gttaaaaact ttaactattt
      541 tggatcaatc atatcagttg ttgatttttc ctggttatta tgattctttg gtgattgatg
      601 aagacagaca gcaaattttg attaatactt tttcgttgac gtcggctcat cttggagagc
      661 taattgatcg aaaaatgcgg ccatttgccc ttaaaaaaat taaacttgaa ttaaataaat
      721 tggcggctga atttggtttt gaatataatc ggttaactct ccgtcgtcag tcaagttgtt
      781 ttggttcctg ctctggccga aataatttaa attttaattg gcagattatt tttttaccct
      841 atcctatttt tcgtcattta ctccttcatg aattaaccca tttatcggtt aaaaaccata
      901 aacgacaatt ttgggatcag ttgaaatttt atgatccgca aactgaagtt catcaccatt
      961 ggttaaaaac taacggccgc cgttgttttc tgtttcttga caaaactgct ctcttttgat
     1021 ataagtgtta acttatgatt gaaaataagg acaattatct tgatcttttt aaaaaaacag
     1081 gtctcgatga ccatattcat tatcaaaact tccgtaatcc atggacgtct agggaagagg
     1141 agattattga tgtttttagc cctttgatgc ggattcaagt cagaaagaag aatcatcaga
     1201 atcctatttt ccatgaggtt tatatttatc ctgatggccg tttaattact ttacccaccc
     1261 ttaaagccat aaccgattat ttagagcaga agcaattagt gagtaatttt ggttttaatg
     1321 aagaaacgaa tacctttaag gccgaggtta attttgaggg cttaaagtat atttgtcagg
     1381 tagttaaagt gataactcaa ccccaatcgg acaatggtca gaaccctgaa cctcgggtaa
     1441 aataaaagcc tttttaatct gatttttaag gttatcagaa acaaagaagt agtcgatccg
     1501 ccagccaatg ttccgttttc gggcaccgct tcggggtgac caccaggaat agccgatttc
     1561 ttggggatga agccatcgat aggtatcgat ataaccattt tgctcgagtt tatccagcca
     1621 ttttctttcg atttctagaa agccggagat attttcattt tctttcggcc gggctaagtc
     1681 aatttcccgg tgagcagtgt tgacatcacc gcaaaaaaca attttttcgc cttgatctct
     1741 taatttattg atgtattgta agaagtattc gtaaaattcg agtttataca ttaatctgcc
     1801 ccgatctttt ttcccgttgg gaaaataaac atttagcaaa gtaaatttat caaacttagt
     1861 aatgattacc cgcccctcgt catcccaatc actatcccga tcaccaataa tgactgcctt
     1921 gggctccaat ttactcaaag ttaaaactcc cgaataaccg gcttttttag cactattcca
     1981 ataacctgta tattctttta aacgcagatc aatttggttc tgactcgctt ttacctcctg
     2041 tagacaataa atatctgctt tatccttttt aataaaatca accaaacctc tcttaaccgc
     2101 tgcccttatt ccatttatgt tccatgaaat tattcgaata ctcatagtga gtattataaa
     2161 ttatagacgg attatctatt ttagatgaag atgattgggg aaatggggaa tttaaaatag
     2221 gggtttatgg taaaatataa ccattccagc ggtagcaaag cggttgatgc agcagtctgt
     2281 aaaactgcct taggtaggtt cgactcctac ccgctggact gtgtatttga gtcaaatcat
     2341 tcttgaaaat tttcgatctt attcccaaaa agttttcgag ttcgatccaa gtgttaatct
     2401 gattattggt caaaatggtt tgggtaaaac taatcttttg gaggcgattt attttgtagc
     2461 taccggtaag agttttcggg ctttgtcttt aaggcagttg atttattggg gtaaagatat
     2521 ggctgatatc cgagcgcaga ttaaagattc tcagggaggg gataaggaat tggaagttca
     2581 actagtaact gtcagacagt tgactactgc taaaacaact cgcaaatttt atttgaatgg
     2641 agtggagaag acccgtagtc attatttggg gctcttatca gtagttgctt ttcagcctga
     2701 agatattcgt ttaattgccg gttcacctac ccgtcggcgc catttacttg atgaagtttt
     2761 atcccagtta gattggcatt atcgagtagc tctctttcaa tatgaaaaag ctttaaagca
     2821 tcgaaatgaa cttttggatc aaattcgcct gggtaaaaac ttaaaggcgg aattgtttta
     2881 ttgggataaa tcattgatta aaaacgatga aattattcac cattaccggg gtaaatttat
     2941 tcagtcggcc aacgattttt tctcctctca tcctgatgct gaaattaaaa ctatttttct
     3001 taactttcaa ccttccgttc tttctcaatc tgttttggat aagaactacc agaaagactt
     3061 aacttggggt tatactagtg ccggtaacca ccgcgatgat tttagttttg ataatttaaa
     3121 ttttcccgat caatatagga atttagaata ttgggggagt cgaggtgaac aacgcttagc
     3181 cgttttagct ttacgtttag ctcaaattgc tttttatgag caaactcatc aggagaaacc
     3241 aattttgttt ttggatgatg ttttttctga acttgatccc aaccaccggc agctggttat
     3301 taaaatttgc cagcattacc agactttttt tacttttacc agtcatgata ttgatcctct
     3361 tttacctagt gcaaaagtga ttaatttata atataatagc caatgcgtcc gactattcag
     3421 acgggctctt taaaattttt gaaggttaat ttgtttggag ccacagctac cagtaccaga
     3481 aattttttta atttgaaaaa ttttggacta agagtttgat cgtggctcag ggttaacgct
     3541 ggcgatgtgc ttaagacatg caagtcgagc gggatttgtg cataacacac ggaatttaaa
     3601 tttctaattt ttaattaaaa actaaaaatt tcgtgtgtta tgcacgagtt cagcggcaaa
     3661 cgggtgagta aaaaatacga acatacctcg gagaggaaca tagcccgccg aaaggcgggg
     3721 taattttcca tgtggtgcct tacggctcca aagacg
//
LOCUS       DAOOIE010000045         3681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_19447_length_3681_cov_3.763100, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000045 DAOOIE010000000
VERSION     DAOOIE010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19447_length_3681_cov_3.763100"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1488)
                     /locus_tag="PK131_01730"
     CDS             complement(<1..1488)
                     /locus_tag="PK131_01730"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HPD44878.1"
                     /translation="MEILIDWLWQLGTIIAVIAAIILLLALALFILIIWWRSKDREKE
                     SLKMVTLQVAVPQDNEVKIDAMEQIIATFSSLRQSGRFKWMEKFIYQPVVSLEIIGTN
                     KDIRFYVCVPEKYRDLIEKQIFGVFNGGDIQVVEEPNIYTENGKVEYAWLGLKKSPYY
                     PLKTYKELAVDPLAALTSALSKLSENETAIIQLVISPADGRWAKAGRSFLSSTKKSEA
                     NPEKASFKIDAKQMEAIENKIGKQGFETAIRIVSIAPTAEIAKVNIGNIKSCFTQFES
                     PYNKLTKKPIRLKNLFMQNFIYKYPVIFWPKVPTILSSDEIASIFHFPNKTIETPNIF
                     WLKSRKAPAPADTPLSGIYIGDNTYRGITKKFYMGDIDRQRHFYIVGQTGVGKSWLLA
                     DMALQDIKAGKGVCFIDPHDTFESILERIPPERADDVIYFDPSNTERPMGFNVMECDN
                     DEQKDFVTSSIINLMYKLYDPYKTGIVGPRFEHAIRNIMLTVMTEP"
     gene            complement(1491..1832)
                     /locus_tag="PK131_01735"
     CDS             complement(1491..1832)
                     /locus_tag="PK131_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44879.1"
                     /translation="MLENKERLPVTTPEQLPQPRKTSKEVIETIERKVFVPKEVKPWL
                     EKIEEEENPIIPTALPTSQKPYLKTVLPISKEVFLKGLNQKVSDAGHWLATFVLRIIK
                     IKGGRVQFQEK"
     gene            1860..2846
                     /locus_tag="PK131_01740"
     CDS             1860..2846
                     /locus_tag="PK131_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPD44880.1"
                     /translation="MDLLNSYQAVVWPFIENFLSENLPSGFHQEITMDYPRRKGKYLR
                     PSLVLITAGAMGFDPHRPAAIATAAAIQISEDWILNHDDIEDDSLERRGAPALHRRFG
                     IPLAINAGDTLQALNWQLIYRLKNATIFDEFSRILNLTLLGQTIELKWIRDQNFNLNL
                     KDVYRIIESKTCYYTIAGPMRLGAILAGATPQQLQILYKFGLYLGKSYQIIDDYLDLT
                     SDFGGQKKQIGNDLYEGKRTVILVDLLRKDKDSKLLSILSKSREEKTKEEINFLLERI
                     KKAGSLEFAYSLANKFANKAKKIFNQELTFLVHQPYRDQLAQVIDFVVNRRQ"
     gene            2843..>3681
                     /locus_tag="PK131_01745"
     CDS             2843..>3681
                     /locus_tag="PK131_01745"
                     /inference="COORDINATES: protein motif:HMM:NF015197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HPD44881.1"
                     /translation="MIDSNFIKAVLERYSGIVGAGIFVLPLLFYQSNFYFALLGLVII
                     TLGMIILDRLYVSIILDTSGHHQLPGYARIYLGRFWGFLALANMLILGLGAMAAYVQL
                     GTGFIQLLLSNSPFLICQLIFLAFLGAFHAKGSRLVGKITHYIPLTGLVIVLFLFAAS
                     LKVPFNFTLPSSNYAFLGPLVFALTGFVIIPDVERLLSKDKDKKIKLIWATALGTLLA
                     ALTYGLFTIAVILLSGSYLSADTVSGLFKVSSKLGQISAILGILLTFKGCLNFLTSLE
                     EVFL"
BASE COUNT          996 a          705 c          722 g         1258 t
ORIGIN      
        1 gggttccgtc attacggtca gcatgatatt tctaatggcg tgttcaaaac gggggccgac
       61 aataccagtt ttataaggat cgtaaagctt atacatcaga ttaataatag aagaggtgac
      121 gaagtctttt tgttcatcgt tgtcacattc catgacattg aagcccattg gccgctcggt
      181 gttggagggg tcaaaataga taacatcatc agcccgttct ggcggaattc tttctagaat
      241 gctttcaaaa gtatcatgag gatcgataaa acagactcct ttacccgcct taatatcttg
      301 gagagccata tcggccaaaa gccatgattt accaacacca gtttgaccaa caatataaaa
      361 gtgccgttgg cggtcaatat ctcccatata aaattttttg gtaattcccc ggtaagtatt
      421 atcaccaata taaataccgc taaggggagt atcagctggt gccggtgcct ttcttgattt
      481 taaccaaaaa atattaggag tttctatggt tttattggga aagtgaaaaa tggaggctat
      541 ttcatccgat gacaaaatag tcggtacttt cggccagaaa attactgggt atttatagat
      601 aaaattctgc atgaataaat ttttcaaccg aattggtttt ttggttaatt tgttgtaggg
      661 agattcaaat tgagtaaagc aggatttgat attaccgata tttaccttgg caatttcggc
      721 tgtaggggca attgagacaa ttctaatagc agtttcaaaa ccttgtttac caatcttgtt
      781 ttcgattgcc tccatttgtt tggcatcaat cttaaacgag gctttttccg gattagcctc
      841 gctttttttg gtgctactga gaaaactgcg accggcctta gcccagcggc cgtcagccgg
      901 agaaataact agttggatga ttgccgtttc gttttcgctt aattttgata aggccgaagt
      961 aagggcagcc agtgggtcaa ctgccagctc tttgtatgtt tttaatggat agtatgggct
     1021 tttttttaat ccaagccagg catattcaac cttgccgttt tcagtataaa tattgggttc
     1081 ttctactacc tgaatatcgc ccccattgaa aacaccaaaa atctgtttct caattaggtc
     1141 gcggtacttt tccggaacac agacataaaa acgaatatcc ttattggtgc cgattatttc
     1201 taaagaaact accggttgat agataaattt ttccatccat ttgaagcggc cgctttgacg
     1261 caagctggaa aaagtggcaa taatttgttc catagcgtcg attttaactt cattatcctg
     1321 agggactgct acctgcagag taaccatttt aagagattcc ttttcccgat ctttggaccg
     1381 ccaccaaata attagaataa aaagggctag ggccagcaaa agaataatag ccgcaataac
     1441 ggcaataatt gttcccagtt gccaaagcca atcgatcaga atttccataa ttatttttct
     1501 tggaactgga ccctacctcc tttaatctta attattctga gaacaaaagt ggctaaccag
     1561 tgcccggcat cactgacctt ttgatttagg ccttttagga aaacctcctt acttatgggt
     1621 aaaactgtct taaggtatgg tttctggctg gtcggtaacg ccgttggtat aattgggttt
     1681 tcctcctcct caattttttc cagccaaggt ttaacctcct tggggacaaa aacctttctt
     1741 tctatagttt caataacttc ttttgaagtt ttccgcggct gaggcaattg ctccggcgta
     1801 gttactggta atctttcttt attttccaac atatttctta ttccagtata taataattta
     1861 tggatttact aaatagttat caggcggtgg tctggccctt tatagaaaat tttttatcgg
     1921 aaaatttacc ctcgggcttt caccaggaaa ttaccatgga ttatccccgg aggaagggga
     1981 aatatctccg gccatcgttg gttttaatca cagctggagc tatgggtttt gatcctcacc
     2041 ggccagcggc cattgctact gctgctgcta tccagatttc tgaagattgg attttaaacc
     2101 atgatgatat tgaagacgat tctttagaac gccgcggggc gccagccctt catcgccgtt
     2161 ttggcatccc tttggcaatt aatgccggcg atactctcca ggctctcaac tggcaattaa
     2221 tttaccgttt aaaaaatgcg acaatttttg atgagtttag tcgtattctt aatctaactt
     2281 tgttgggtca gacaattgaa cttaagtgga ttcgagatca aaattttaat ttaaatttga
     2341 aagatgttta tcggattatt gagtcaaaaa cctgttatta cactattgcc ggaccaatga
     2401 gattaggagc gattttagcc ggagctaccc cccaacaact ccaaattctc tacaaatttg
     2461 gcctatattt gggaaaatct tatcaaatta ttgacgatta tcttgattta acttccgatt
     2521 tcggcggtca aaaaaaacaa atcgggaatg atctctatga gggtaaacgg acagttattt
     2581 tggttgattt actccggaaa gataaagatt caaaactttt atctatttta tctaaatccc
     2641 gggaggaaaa aaccaaagaa gaaatcaatt ttcttctgga gaggataaaa aaggctggta
     2701 gtttggaatt tgcctattct ttggccaata aatttgccaa taaggccaaa aaaattttta
     2761 atcaagaatt aacctttctt gtccatcagc cttaccgcga tcagttagcc caggttatcg
     2821 attttgtcgt taatcgccgt caatgataga ctcaaatttt attaaagcgg ttttagaacg
     2881 ttatagcggt attgttggag cgggaatttt tgttctccct ctcctttttt atcagtctaa
     2941 tttctatttc gccttattgg gactggtaat aattactttg gggatgatta ttcttgaccg
     3001 tctttatgtc agtattattt tggatacttc tggtcatcat caattgcccg gctatgcccg
     3061 gatttatctt ggccggtttt ggggtttttt agctctggcc aatatgttaa ttttgggttt
     3121 gggggcaatg gcggcttatg ttcaactcgg gaccggtttt attcaattac tcctatctaa
     3181 ttcaccattc ctaatttgcc aattaatttt tcttgctttt ttaggtgctt ttcatgctaa
     3241 aggttcccga ctagttggga aaatcactca ttatattcca ttaacaggat tggtaattgt
     3301 cctgtttctt tttgccgctt ctttgaaagt tccttttaat tttactctac catcatctaa
     3361 ttatgccttt ttaggaccgt tggtttttgc tctgaccggt tttgtaatta tccctgatgt
     3421 agaacgcctt ttaagcaagg ataaagataa aaaaattaaa ctaatctggg caactgcctt
     3481 aggaactctt ttagcggcct taacttatgg tctttttact atagcagtta ttcttttgtc
     3541 tggttcttat ttaagcgccg atacggtgag cggcttgttt aaagttagtt ctaaattagg
     3601 tcaaattagt gctattttgg gtattttgtt aacctttaaa ggctgtctta attttttaac
     3661 tagtttagaa gaggtttttc t
//
LOCUS       DAOOIE010000046         3671 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_19518_length_3671_cov_3.565265, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000046 DAOOIE010000000
VERSION     DAOOIE010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19518_length_3671_cov_3.565265"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PK131_01750"
     CDS             complement(<1..321)
                     /locus_tag="PK131_01750"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="capsular polysaccharide biosynthesis protein;
                     catalyzes the formation of D-mannose 6-phosphate from
                     alpha-D-mannose 1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase CpsG"
                     /protein_id="HPD44882.1"
                     /translation="MTQIDPSIFKSYDIRGVYPTQLDEEVAYKIGRAYGTFIQNENPG
                     RQLTIVVGRDMRLSSLSLKEKLVAGLLDQGLNVVEIGLTSTPTMYFATAFYGYDGGVE
                     ISASH"
     gene            complement(346..1392)
                     /locus_tag="PK131_01755"
     CDS             complement(346..1392)
                     /locus_tag="PK131_01755"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPD44883.1"
                     /translation="MTIKVKIRKIVGFIVIVVVVILIGVGFKKGKGEIEKNPIRNEEI
                     KPNSPPEVVLIAVGDISFSRGVERMVKKQNDINYPFEKIEAYLNSADLVFGNLETPIT
                     TGREIQTGEMIFRSNPGTEQALKRAGFSILSLANNHTPNFGEKGLKDTFNYLTETGIS
                     YVGAGENSSEAYQPVYLEKKGITFAFLAYNDTDVVPVSYEATDIRAGTAFMRIDRMTA
                     AVKEAKQQADFVIVSMHAGDEYTPQPNQSQLNFAHAAVDAGADLILGHHPHVVQTMEK
                     YREKYIFYSLGNFVFDQMWSAETKEGLAVKINFSPDKINNISFFPIVMENLGQPREAT
                     GSEAAKILSRLEPI"
     gene            1489..2058
                     /locus_tag="PK131_01760"
     CDS             1489..2058
                     /locus_tag="PK131_01760"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPD44884.1"
                     /translation="MKGQTPKKQIIKTARNLFSESSYAGVSMSDIAKKLNVTKTALYY
                     HFAGKAEIYQKVLDEVFTDLSSMIKKVAEEKNARKRLRRLIKNYLDFGLKEKNLVKGV
                     MIKIPSADNRITQHITKLKEQIIELIRPTIEEALSNGKTISESNSRLLTFLLTGMMDS
                     LLLEYSFLNKEISPKKMSNQIIALLFPSH"
     gene            2059..3546
                     /locus_tag="PK131_01765"
     CDS             2059..3546
                     /locus_tag="PK131_01765"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPD44885.1"
                     /translation="MNLNSSLKSKTGKAFWDLISNVSPQVAKNLAKRPLARKTILGLM
                     EKNLRRTTLENPNYPYQVQADKFYMGRALLKSLDKVLTQINRHPTYNRSLYNSVLPKG
                     LHAWQTGKEKIEAFKDKYGFCPPGFLTVSPGKLCNLKCTGCYANSSSAACEKLDWETV
                     DRIITEKSQDWASWFTVVSGGEPLLWQSQGKTIIDLAKNHQDNFFLIFTNGTLIDKKM
                     ARKLAETGNITLAISVEGFAKETDERRGQGTHKRILKAMKNLRDEGIPFGISLTATRK
                     NAELITSEKLIKYYWDRGASYAWIFQLMPIGRGSMDLVVTPEQRLKMFRRTQKLIRKG
                     YFIADFWNCGAVVSGCISAGRPTGGGYLYLEWNGNITPCVFNPYAVGNINEIYKKGGS
                     LNDVLSSNFFTKIRQWQRDYGLGKKPEEIGNWILPCPIRDHYREMKQFIKETKPKPID
                     EAADQALKDKEYEKKMIEYDRELAKILNPVWEKEYLKKRRDNCRH"
BASE COUNT         1217 a          714 c          684 g         1056 t
ORIGIN      
        1 atgtgaagcc gaaatctcaa caccaccatc atagccgtaa aaagctgtag caaaatacat
       61 cgttggagta ctggttaaac caatttcaac cacattcaat ccctggtcaa gcaaaccagc
      121 aaccaacttt tcttttagag acagactgga gaggcgcata tcacggccaa caacaattgt
      181 taattgtcga ccgggatttt cgttttgaat aaaagtacca taggctcggc caattttata
      241 ggcgacctcc tcatccagct gggtgggata aacacctcga atatcataag atttaaaaat
      301 agaaggatca atttgggtca tagtatttta aggataacat aaaaactaaa ttggctctaa
      361 tctagataaa attttggctg cttcacttcc ggtggcctct cgaggttggc ctaaattttc
      421 cataactata ggaaaaaacg atatattatt aattttatcg ggagaaaaat taatcttgac
      481 ggctaaacct tctttggttt ctgccgacca catttgatca aaaacgaaat tacctaaact
      541 ataaaaaata tatttttcac ggtatttttc catagtttga acaacatggg gatggtgacc
      601 gagaattaaa tcagccccgg catcaacggc cgcatgagca aaatttaact gagattggtt
      661 aggttgagga gtatattcgt cgccagcatg catcgaaaca atgacaaagt cggcctgttg
      721 tttggcttct ttaacagcgg ctgtcatcct atctattcgc ataaaggccg ttcccgcccg
      781 aatatcagta gcttcataag aaaccggaac gacatcggta tcattatagg ccaaaaaggc
      841 aaaagttatt cctttctttt ctaaataaac tggttgataa gcctctgaac tattttcacc
      901 agcccccaca tagctaatgc cagtctctgt aagataatta aaagtatctt ttaatccttt
      961 ttcgccaaaa ttgggggtat gattattagc caaagataaa atagaaaaac cagctcgttt
     1021 cagagcctgt tctgttcccg ggtttgatct aaaaatcatt tcgccggtct gaatttcccg
     1081 cccggtcgta atcggagttt ctaaattgcc aaaaaccaag tcggcgctgt ttaaataggc
     1141 ttcaattttc tcaaaaggat aattaatatc gttctgtttt ttaaccatcc gttccacccc
     1201 ccgggaaaaa gaaatatcac cgacagcaat taggacaacc tctggcgggg aattcggctt
     1261 tatttcttca tttctaatcg gattcttctc tatttcacct tttccttttt tgaaaccaac
     1321 gccaatcaag ataaccacca caacaataac gataaatccg acaatctttc tgattttcac
     1381 ttttattgtc attatcgagt cttcacctac ccatgtcaac ttgaggatta ttgacaattt
     1441 ttctccttcc ttgctatact atctaattaa cttaccggcc ggtaaaaaat gaaaggccaa
     1501 actcctaaaa aacagattat aaaaacagct cgaaatcttt tttccgaatc cagttatgct
     1561 ggggtttcca tgagtgatat tgccaaaaaa ctgaatgtga ccaaaaccgc cctttattat
     1621 cattttgccg gtaaagctga aatttaccaa aaggtccttg atgaggtttt caccgatttg
     1681 agttcaatga ttaaaaaggt tgcagaagaa aaaaatgccc gaaaaagatt aaggcggctc
     1741 atcaaaaact atttagattt tggtttaaaa gaaaaaaatc tggttaaggg agtaatgata
     1801 aaaataccgt cggctgataa tcgcattacc cagcacatta ctaaattaaa agaacaaata
     1861 atagaattaa ttcgaccgac tattgaagaa gccctgtcta atggcaaaac aatatcagag
     1921 tctaatagcc ggttattaac ctttctctta accggaatga tggatagcct tttattggaa
     1981 tactcttttc ttaacaaaga aattagtcct aaaaaaatgt caaatcaaat tattgcttta
     2041 ttatttccct ctcattaaat gaatttaaat tcgtcgttaa aatcaaaaac cggtaaagcc
     2101 ttttgggatt tgatcagtaa tgtttcacct caagtagcaa aaaatttggc taaaaggccg
     2161 ttggcccgga aaactatttt agggttaatg gaaaaaaatt tgcgtcgaac cactctcgaa
     2221 aatcctaatt atccctatca ggtccaggca gataaattct atatgggccg ggctcttctt
     2281 aaatcgttgg ataaagtttt aacccaaatt aatcgtcacc caacttacaa ccggtcactc
     2341 tataattcag ttttgcccaa aggtttacac gcttggcaaa caggcaagga aaaaattgag
     2401 gcatttaaag acaaatatgg tttctgccca ccaggatttt taaccgttag tccgggtaaa
     2461 ttatgcaatt taaaatgtac aggctgttat gccaatagtt cttcagccgc ctgtgaaaag
     2521 ttagattggg aaacggtgga ccggattatt accgagaaaa gccaagattg ggcctcctgg
     2581 ttcacagttg tttccggagg agaaccatta ttatggcaaa gtcagggaaa aacaatcatt
     2641 gatctggcga aaaatcacca agataatttt ttcctaattt ttaccaacgg cactttaatt
     2701 gataaaaaaa tggctagaaa actggccgaa acgggcaata ttactttagc tatttcggtc
     2761 gagggttttg ctaaagaaac cgatgaacgc cgtggtcagg gcactcataa gagaattctt
     2821 aaggcgatga aaaatttacg ggatgagggt atcccttttg gtatttccct aactgctacc
     2881 agaaaaaatg ctgaattgat taccagtgaa aaattgatca aatactattg ggacagaggc
     2941 gctagctatg cttggatttt tcaattaatg ccaattggac gaggatcaat ggatttggtg
     3001 gtaacccctg aacaaagatt aaaaatgttc cgccgaactc aaaaattgat tagaaaaggt
     3061 tattttattg ccgatttttg gaattgcggg gcagtggtaa gtggttgtat ctcagccggc
     3121 cggccaactg gcggcggcta cctttatctc gaatggaatg gtaatattac cccttgtgtt
     3181 tttaaccctt atgccgtcgg caatattaat gaaatctata aaaagggcgg tagtttaaat
     3241 gacgttttaa gctccaattt tttcactaaa atccgacagt ggcaaaggga ctatggtttg
     3301 ggtaaaaaac cggaagaaat tggtaattgg atcttaccct gccctattcg agatcattat
     3361 cgggagatga agcaattcat aaaagaaact aagcctaaac caattgacga agcggcagat
     3421 caagccttaa aagacaaaga atatgagaaa aaaatgattg aatatgatcg ggagttagcc
     3481 aagatcctga atccggtttg ggaaaaagaa tatctcaaaa aacgccgaga taattgccga
     3541 cactaatcct catttttgat ataattacct aaagatttgc ctctatgaat caaactaatt
     3601 ttggtgatta cgccgttttc gtcaaggata accctgaaaa agaaaaaaaa cgactcgaaa
     3661 aaaagttaga t
//
LOCUS       DAOOIE010000047         3670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_19523_length_3670_cov_7.825173, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000047 DAOOIE010000000
VERSION     DAOOIE010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19523_length_3670_cov_7.825173"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(92..250)
                     /locus_tag="PK131_01770"
     CDS             complement(92..250)
                     /locus_tag="PK131_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44886.1"
                     /translation="MKEKSDNIKGLLIWVPDDLKRKFKAAVIANGQSLKSVLVSFIQD
                     YIEKSGKQ"
     gene            complement(315..2018)
                     /locus_tag="PK131_01775"
     CDS             complement(315..2018)
                     /locus_tag="PK131_01775"
                     /inference="COORDINATES: protein motif:HMM:NF015154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HPD44887.1"
                     /translation="MLKPLKRDRKNCFSTLDIETAPDGSVLDIAIFNGEKVTYFTGWR
                     EFFIYLQAVVSDDKRYQKFLAHSGGGFDYVSMIEFLLNNKKDFRFTFEVILSGSDIVL
                     IELFIGDKKVQFIDTYKTLPASLANLSELFNVTTKKQKIEKSNYKDMRKFKTDDPDLY
                     YKYLGDDVISLFEIAIAFEKILDIEFFPVTAASLALYLFRRKFQTINLFKVKPEIDEF
                     ISNAYAGGRVECFEAGEFKDIKVYDVNSLYPSVMLTNEYPIGTPVKVSTFKSGKIGVY
                     HIRFFQKDRTKPAVLWVKTKDTGLTFTYAGEGYFFNPEIEEALRQGVEIEILEGYVWI
                     RSINLFKDFVEYYYKLRLKNKNNAIGYCCKIILNSLYGKFGQKENKSVLRRVLDHDEL
                     MKLIKNKNYEVKVYKATAALYEITYNRPIDHRLIQLAAYTTSLARIRLYQALSDNVIY
                     CDTDSVHIKDGNFRGRISDKIGDWKLENSGEGIYIGRKQYWLNGKAKFKGVKEFDILG
                     KGEVLIKKDDYLKILNGESLGKKFLSFPKLKSVISGSPACRPYFITKTLEKGRYLSSR
                     K"
     gene            complement(2002..2601)
                     /locus_tag="PK131_01780"
     CDS             complement(2002..2601)
                     /locus_tag="PK131_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44888.1"
                     /translation="MVSYKQKIKYIERRVGQKNLSEILGLNDRTIRRYKEGTRRPSSQ
                     VKENIDLTYNILKQKVKRPVKGRVQKAKVFNKVKPELVKKVSPVIRPEFKYRIEKFKE
                     AQTEHLIITIHDINSISSTIDYLIKQKCEAAYLVIRAKKAGSKKVLTYQTSIWEINDF
                     KGNYLADIAGIIGKYKINIFNNLGEVDIDVVGVRYAETS"
     gene            complement(2670..2993)
                     /locus_tag="PK131_01785"
     CDS             complement(2670..2993)
                     /locus_tag="PK131_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44889.1"
                     /translation="MKKKIAGFSVAKKFVTEGERLEGMIVEKKVIEIGGKKQEVVVMR
                     DKDDLEWTLFISAGLRPLLDLSNGEFVSIIYRGSKKLKDGKTFKLFDIYLLDSLDSES
                     NDLPF"
     gene            complement(2997..3302)
                     /locus_tag="PK131_01790"
     CDS             complement(2997..3302)
                     /locus_tag="PK131_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44890.1"
                     /translation="MLTLFIFILLLIAAVSIILLWNKIEQIRNELLRWISVTEILRDD
                     IKAQIEACGENEIFLKHKLKDRLFIVNLLLTRLTRPEVIKNLVKDSCEFKLNSKKGD"
     gene            complement(3302..3520)
                     /locus_tag="PK131_01795"
     CDS             complement(3302..3520)
                     /locus_tag="PK131_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44891.1"
                     /translation="MTEKELKEKVAVTIEVTKIDYLILQELAKEQGKAVEIYISDELA
                     GAVREWANNYLEQLVSEAEIKAGLKKVN"
BASE COUNT         1113 a          772 c          539 g         1246 t
ORIGIN      
        1 ataaaaatct gctttaaaga gagatcatcg tcaatcgctt ttttctttac tgacttataa
       61 agatcatctg gcacttgtat aaggatatgt ttcattgttt ccctgatttt tcgatataat
      121 cttgaataaa agaaaccaag acggatttaa ggctctggcc gttagcaata accgccgcct
      181 tgaatttccg tttaagatca tctggtaccc agatcaacag gcctttaata ttatcagatt
      241 tttctttcat tttaacctct ctttaaagat aacattttat aaagattagt caacctgttt
      301 tttataataa cttatcattt ccggcttgat aaataccgcc ctttttctaa tgttttagtg
      361 ataaaataag gtcggcaagc tggagagccg ctaataacag attttaattt cgggaaactt
      421 aaaaactttt tacctaaact ctccccgttt aggattttta aatagtcgtc ttttttgatt
      481 aaaacctcgc ccttacctaa aatgtcaaac tcttttactc ccttaaactt agctttccca
      541 ttcagccaat actgcttacg gccgatataa ataccctcgc ccgaattttc aagtttccaa
      601 tcgccgattt tatctgagat ccggcctcta aagtttccgt cttttatatg gacagagtca
      661 gtgtcacaat aaataacatt atcactcaaa gcctggtaaa gacgtatcct ggctaaagat
      721 gtagtataag cggctaattg aataagccga tgatcaatag gccggttata ggtgatctcg
      781 tataaggccg ccgtcgcttt ataaaccttt acctcgtagt ttttgttttt aattaacttc
      841 atcagttcat cgtgatcaag gacccggcgt aaaaccgatt tgttttcctt ctggccgaat
      901 ttcccataaa gagagttaag gataatttta cagcagtaac cgatagcatt atttttattt
      961 tttagtctaa gtttataata gtattcaaca aagtctttaa aaagattaat cgatctgatc
     1021 cagacatagc cttctaaaat ctctatctct acgccttgcc ttaaggcctc ttcaatttcc
     1081 ggattgaaaa aatatccctc tccagcataa gtaaaagtca atccggtatc tttagttttt
     1141 acccataaaa ccgctggctt agttctgtct ttctgaaaaa atcggatatg ataaacgcct
     1201 atcttaccag acttgaaagt agaaacttta accggagtcc cgatcggata ttcgttagtt
     1261 aacatcactg acggataaag cgaattaaca tcataaactt taatatcttt aaactctccg
     1321 gcttcaaaac attctacccg cccgccagca taagcgtttg agataaattc gtcaatctca
     1381 ggcttaactt taaaaagatt tatagtttga aacttacgcc tgaataaata caaagctaaa
     1441 gaggccgccg ttacaggaaa aaattcaata tcaaggattt tttcaaaagc aatcgcaatc
     1501 tcaaaaagag agataacatc atcgcctaaa tatttatagt ataaatccgg atcatcagtc
     1561 ttaaatttac gcatatcttt ataattagat ttttctattt tctgcttttt agtcgtcaca
     1621 ttgaaaagct ctgaaagatt agccaaactg gccggtaagg ttttatatgt atcaatgaat
     1681 tgaacttttt tatcaccgat aaaaagctca atcagcacta tatctgatcc ggatagaata
     1741 acctcaaagg taaatctaaa atccttttta ttattcagta aaaactcaat cattgatacg
     1801 tagtcaaagc cgccgccgga gtgtgctaaa aatttttgat atcgcttatc atcagaaact
     1861 acagcctgaa gataaataaa aaactctcgc cagcctgtaa aataagttac tttttccccg
     1921 ttaaaaatgg ctatgtctag gactgatccg tccggtgccg tttcaatatc aagagtagaa
     1981 aaacaatttt ttctatccct tttaagaggt ttcagcatac cttaccccta caacgtcaat
     2041 gtcaacctct cccagattat taaaaatatt tatcttatac tttccgatta tacccgcgat
     2101 atcagcgaga taatttccct taaaatcgtt tatctcccag atcgatgttt gatatgttaa
     2161 aactttttta ctaccggctt ttttagctct gatcaccaga taggctgcct cgcatttttg
     2221 ttttatcaga taatcaatgg tagaactaat agaatttata tcgtgaattg ttataattaa
     2281 atgctctgtt tgagcctctt taaatttttc aattcgatat ttaaattcag gtcttataac
     2341 aggcgatact tttttaacta attctggttt aaccttatta aaaactttag ctttttgaac
     2401 tctgccttta acaggccttt taactttttg ctttaaaata ttataggtta gatcaatgtt
     2461 ttcttttacc tgagagcttg gacgccgtgt cccctcttta taccggcgga tcgttcgatc
     2521 atttaaacct aaaatctcag ataaattttt ctggccaact ctacgctcaa tgtattttat
     2581 tttttgcttg taacttacca ccttttacct cctaataaaa gaaaggccgg cagatagagg
     2641 atacccgccg gcctcaggtg aagggagatt tagaagggta gatcgttaga ctctgaatcc
     2701 aaagagtcta agagataaat atcaaacaac ttaaaagtct ttccgtcttt taactttttc
     2761 gatccccgat aaattattga tacaaactcg ccattactta aatcaagtaa cggccgtaag
     2821 ccggcagata taaaaagcgt ccattccaga tcatccttat ctctcataac caccacttcc
     2881 tgttttttcc cgccaatctc aataaccttt ttctcaacaa tcataccttc taatctctca
     2941 ccttcagtga caaacttttt agccactgaa aaaccagcaa tcttcttttt cattttttaa
     3001 tctccctttt ttgaatttaa tttaaactcg caagagtcct taactaagtt tttaataact
     3061 tctggccgtg ttaatctggt gagtaaaaga ttaactataa aaaggcgatc ctttagctta
     3121 tgctttaaaa aaatttcatt ttccccgcag gcttcaatct gtgctttaat atcatctctt
     3181 aaaatttcag tgacactaat ccatctaagc aattcatttc ttatctgctc aattttattc
     3241 cataacagaa taatcgacac ggccgctatt aaaagtaaaa taaaaataaa taaggttaac
     3301 atcagttcac ctttttaaga ccagctttaa tttcagcttc agagaccaat tgctccagat
     3361 aattatttgc ccactctcta acggcgccgg caagctcatc agagatataa atttcaacag
     3421 cttttccttg ttctttagcc agctcttgaa ggatcagata atcgattttt gttacctcaa
     3481 tggtaacagc tactttttcc tttagctctt tttcagtcat ttttaactcc cttcgccttc
     3541 aagtttatca taggaaaaaa accaatgtca agcgtttttt ttaataattt attatctttt
     3601 tttccttcct ggccggcggc gaggcacgtc gtccggtggc ctgtggcggg ggattcggtg
     3661 gcgccccccc
//
LOCUS       DAOOIE010000048         3667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_19555_length_3667_cov_2.874862, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000048 DAOOIE010000000
VERSION     DAOOIE010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19555_length_3667_cov_2.874862"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="PK131_01800"
     CDS             <1..496
                     /locus_tag="PK131_01800"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HPD44892.1"
                     /translation="LSFLQTIRILFLRVSLPYSIGAVLGTSLIFFICFYGGKPFVEKF
                     GRYIGISWRLIEKVQADFKKTISDELFILIGCSIPVVPVSLITAFCGIFKTKPIKFYP
                     MLFLALLIRATILAFIGFKMGQTFTGLAKGLDKIESILTIIGAFLILGFLFLKREQYL
                     KKNG"
     gene            complement(520..1194)
                     /locus_tag="PK131_01805"
     CDS             complement(520..1194)
                     /locus_tag="PK131_01805"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HPD44893.1"
                     /translation="MKRYLGIILLLVILWFVLPINKGATPRQVTVVGEAKTRQRNQTA
                     IFNAGVSITGVKKDSVVAEANKKVADLIQALKDFGIPEADLKTQSMNLNEEEIYPTKG
                     KQWRAYNSIEIILRETAKAAALADLLAKSGANNIYGPNYSFEDTSMVSKTLYDEAIKD
                     AREKAEMIAKAAGRRLGRVIQVDDSGSSAVYPLYRAAEGLGGGTAIEPGSTTISKILT
                     VSFELK"
     gene            complement(1236..1871)
                     /locus_tag="PK131_01810"
     CDS             complement(1236..1871)
                     /locus_tag="PK131_01810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HPD44894.1"
                     /translation="MRLGGCQQTTFLDYPGKLATTIFTVGCNFRCPFCHNRDLVLEAK
                     EVISEEDFFEFLKKRKNILEGVVISGGEPTLQPGLDNFCSKIKQIGYLVKLDTNGSRP
                     EVLEKLIKNHLIDFVAMDIKADRKNYEKASGVKVDLAQIEKSVEIIKKSGLDHQFRST
                     IVRGIHDKKVVARMKKKFPNLVLQKFRAQNCLNPAYLAVEEWTEAEWCEFA"
     gene            complement(1868..3667)
                     /locus_tag="PK131_01815"
                     /pseudo
     CDS             complement(1868..3667)
                     /locus_tag="PK131_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020587341.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
BASE COUNT         1034 a          880 c          564 g         1189 t
ORIGIN      
        1 tttgtcattc ctacaaacaa ttcggattct gttcttgaga gtttctttac cttattctat
       61 cggtgctgtt ctcggaactt ccctcatctt ttttatctgc ttttatggcg gtaagccgtt
      121 tgttgaaaaa tttggccgtt acataggtat ttcttggaga ttaattgaaa aagttcaggc
      181 tgattttaaa aaaactattt ctgatgaact gtttattctt atcggttgtt ccatcccggt
      241 tgtgccggtg tctttaatca cggctttctg cggtattttt aaaactaaac caattaaatt
      301 ttatcccatg ctctttctgg ctttactcat tcgggctacc attttagctt ttatcggttt
      361 taaaatgggc caaaccttta ctggtttggc taagggatta gataaaatcg aatctatttt
      421 aactattatt ggggctttcc ttattctcgg ttttcttttt ctcaaacggg agcaatacct
      481 caaaaagaac ggctaaatcc cccatccgtc agtcttcccc tattttaact caaaactcac
      541 cgtcagaatt ttactgatcg tcgtcgaacc cggttcgatc gccgttccgc cgcctaaacc
      601 ttccgccgct cggtaaaggg gataaacagc gctactgccg ctatcatcaa cttgaatcac
      661 tcgacctaat cttcttccgg cggctttggc aatcatctct gctttttctc gagcatcttt
      721 tatggcttcg tcataaaggg ttttgctcac catactcgta tcttcaaacg aatagttcgg
      781 cccgtaaatg ttattcgccc cgctcttggc caacagatct gccaaggctg ctgctttggc
      841 cgtttctctc aaaattattt caatcgaatt ataagcccgc cactgcttcc ctttggtcgg
      901 gtaaatctcc tcctcattca aattcatact ctgagtttta aggtccgctt cgggaatacc
      961 aaaatccttc aacgcctgga tcaaatcggc cacctttttg ttggcctccg ccaccaccga
     1021 atcctttttc accccggtaa tacttactcc cgcattaaaa atagctgtct ggtttctctg
     1081 cctagtctta gcctccccca ctaccgttac ctgccgcggg gttgcccctt tgtttatcgg
     1141 caaaacaaac cataaaatca ctaatagtaa aattatgcct aaatatcttt tcatctactt
     1201 attttagcat ttccacaatt caatccgcca ccatattatg caaattcaca ccattccgct
     1261 tcggtccatt cctcaactgc caaataggca ggatttaaac agttctgggc cctgaatttc
     1321 tgtaaaacca aatttggaaa tttttttttc attctggcaa ctactttctt atcgtggatg
     1381 cccctcacaa tagtcgatcg aaattgatga tcaagaccac tttttttaat gatctcgact
     1441 gacttctcaa tttgggctag atcaactttt actccactgg ccttttcgta attctttcga
     1501 tctgctttaa tatccatagc cacaaaatca attaagtgat tttttattaa tttttctaaa
     1561 acttccggtc gactgccatt ggtatccagc ttaactaaat aacctatttg tttgattttg
     1621 ctgcaaaaat tatctaaacc aggctgtaat gtcggctctc ctccgctaat tactacccct
     1681 tcaaggatat tctttctttt ttttaaaaat tcaaaaaaat cctcttcact aataacctct
     1741 tttgcctcta aaaccaaatc tcgattatga caaaaaggac aacggaagtt gcaccctacc
     1801 gtgaagatag tggttgctaa tttacccgga taatccaaaa aggtagtctg ctgacaccca
     1861 ccaagtctca tattttattc aagctgtatg ttttccttag gtgaaattcg gccactttac
     1921 ctttgttcca ctgtgatacc ggccgcaaat agccgacgat cctcgaaaac acctcacact
     1981 tagttctttg ttttagcata ttttttaggt aacttataac ctatttcttg atcacacttg
     2041 ggacagtatt cccattcacc agttaaatac ccatgtttag ggcaaattga aaaggtcggg
     2101 gtaatcgaaa agtaaggtaa atgataattt tgggcaattt tcctaaccaa ttttttaacc
     2161 gattcaatat cgggcaaact ttcactcata aaacaatgta aaaccgtccc gccattatat
     2221 ttcacttgca aatcatcctg atgatctaag gcaacaaaaa tatcgtcagt gtaatccact
     2281 ggcaggtgag tagaattagt ataaaaagga acatcactac cagcggtaat aatatcagga
     2341 tataatcttt tatctaactg ggccaatcgg taagcagctc cctcagctgg agtagcctct
     2401 aaattaaaca tctctccggt ttctttttga tataacaaaa tttttccccg catataatcc
     2461 attacctcta aggcaaattc tttcccttcc ggatcggcaa tattttttcc tattaaattc
     2521 agcaatgctt cattcattcc atttaaacca atagtattaa aatggttttt ccaataaaca
     2581 ccatgccgct gataaacatc ccgaagataa taagcgcaat aaggatacaa accttgccga
     2641 gtcagcaatt ccacctcagc tcttttcgcc attaacgaat cttttgaaag atccattaac
     2701 ttacccaaca tagtgtaaaa ctccttcttg gcttgctctt tattcttggt ttctttttta
     2761 gctaaataac ctaatcgagc catattaata gttaccactc cgattgctcc tgttaaagga
     2821 ttagcagaaa atagtcctcc tctctttctt aactcccgat tatccagtcg taaacgacaa
     2881 cacatcgatc gagcatcatc cggatccata tctgaattta caaaattaga aaagtagggc
     2941 aaaccatatt ttctagtcat ttcccaaatc gaatcccaat cctttttatt ccagtcaaaa
     3001 ttctttgtaa tcgagtaagt cggaattgga aaagtaaata ccctaccctg ggcatcccct
     3061 tcccccataa cctcggctaa agcctgatta aaaatatcca tctctttttg aaaatcctta
     3121 tatttagcct tgatcggttt gccgccaata atcaccgcct cctcacccac catttttgtt
     3181 ggctggagat ctaaactaac attagtaaag ggtgtttgaa atccaactcg cgtcggcaca
     3241 ttcatattaa aaacaaactc ctgtaaagat tgttttactt gttgataatt caaatgatca
     3301 gttcgaataa acggggctaa ataagtatca aaattagcaa cagcaaccgc accggcaacc
     3361 tcaccttgaa tagtgtaaaa gaaattaact aattgcccta aggctgtccg taaatgtttt
     3421 ggcggccaac tactgacttt actttccacc cccccgaagc ctctaatcaa caaatccttt
     3481 aaatcccaac cggtgcaata aggtgataaa gaagctaaat catgaatatg aatatccccc
     3541 gagagatgag cttttctgat ctcgcgactg taaatcttat tcatccagta attggctgaa
     3601 ataattgagg caacatggtt attaagacct tggagcgagt aagtcatatt agcattctct
     3661 ttgaccc
//
LOCUS       DAOOIE010000049         3654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_19654_length_3654_cov_4.187552, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000049 DAOOIE010000000
VERSION     DAOOIE010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19654_length_3654_cov_4.187552"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="PK131_01820"
     CDS             <1..202
                     /locus_tag="PK131_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44895.1"
                     /translation="KDWRELAKKWGVNYLIPVGGKPDSKVLVTFLDEVDEEGLEPIES
                     LKVDKDNLPDGLEIVLLKVEEK"
     gene            199..1503
                     /gene="dnaB"
                     /locus_tag="PK131_01825"
     CDS             199..1503
                     /gene="dnaB"
                     /locus_tag="PK131_01825"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPD44896.1"
                     /translation="MSDLLKLPPSSVETEMSVLGSILIDNDALLAVTDFLRPEHFYES
                     KNAKVYKAMLSLFENRNPIDIITVAEKLKEMKELKNIGGKTFLVKLANEVPTAANVAS
                     YGKIIKALAAKRDLISAAARITEKAFDESLPSEQLLDLAEQEIFSLSQRHIKNTPVSL
                     KEVLTASFDRLDELQKLGSGLRGVPTGFTSLDKMLAGMQNSNLLILAARPGIGKTAFV
                     LNVGRHVAVEEKLPTVIFSLEMSKEELVDRLLVRQGLIDAWKLKTGQLSDDDFTSLSE
                     AMGVLAEAPLFIDDTPGLTVTELRTKSRRLQLDKGIKFIIVDYLQLMHGQTKDNRVQE
                     VSEISQGLKNLARELKVPVLAAAQLSRAMEARGGRPRLSDLRESGSIEQDADVVMFLH
                     REDEDIKEMVTLSIEKHRNGPTGQMNLFFNGKQVSFFDVEKK"
     gene            complement(1507..1590)
                     /locus_tag="PK131_01830"
     tRNA            complement(1507..1590)
                     /locus_tag="PK131_01830"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1554..1556),aa:Leu,seq:caa)
     gene            1657..2274
                     /locus_tag="PK131_01835"
     CDS             1657..2274
                     /locus_tag="PK131_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44897.1"
                     /translation="MYFVFWLIAIVLFLYLSWKQLQEGYNSQQIIGFSWGLVLAMILI
                     GRLTGGEWQSDSGWLILAIWRPLRVNYFVSLIIAVLLCAWMSRKNNWKLWSVLEDLTP
                     AFLAFITFGLMADLCRAFTWKLLIDLAIAIMAVVWSRGLKSRYRSLVWYPSGKKGFVF
                     LAISTINFALISISTFFLEDRHFFPYLYLGTSLISGWGLYMLGRR"
     gene            2271..2642
                     /locus_tag="PK131_01840"
     CDS             2271..2642
                     /locus_tag="PK131_01840"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HPD44898.1"
                     /translation="MNNNELNLPKKLLEPIRLFLEREIVRLKRAEKKLKKADPFKDNQ
                     RTNDNSLEEDVDEQIGHFDKQVKVKFLNRQIVQLRKALTMIKLGKYGICEKCGKMIDT
                     DRLAVKPDTTLCIKCEREQEK"
     gene            2823..3470
                     /locus_tag="PK131_01845"
     CDS             2823..3470
                     /locus_tag="PK131_01845"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPD44899.1"
                     /translation="MEEKTTPEVIYEDQNILVLDKPAGWVTTKENNHSERNIERWLEK
                     MDKNNLPRQGIVHRLDKGTSGVLIVAKNSQTLADFKKRFRQRQVEKRYLAWIEGDLPA
                     EGDINLPIGRSKYNREKFQVSEAGKKAQTKFKVLRKINIGGRIYSLVDVNLITGRTHQ
                     IRVHFSHLGWPIVGDVVYGSKNKTVDRPELHCYQIKIAGFTFESPFSENFYEKTQ"
     gene            3454..>3654
                     /locus_tag="PK131_01850"
     CDS             3454..>3654
                     /locus_tag="PK131_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44900.1"
                     /translation="MKKHSRRINRIAWLIFGGLILLLALAFLENDFKWDGKRDMRIGI
                     ITDENLAVLVISPERRMINRVET"
BASE COUNT         1145 a          499 c          857 g         1153 t
ORIGIN      
        1 gaaagattgg cgggaattgg ctaagaaatg gggagttaat tatttaattc cggttggagg
       61 caaaccagac agtaaagttc tagttacttt tttggatgaa gttgatgaag agggattgga
      121 accgatagag agtttgaaag ttgataaaga taatcttcca gatggtttag aaattgtttt
      181 attaaaggta gaagaaaaat gagcgattta ctcaaattac cgccgagttc tgttgaaaca
      241 gaaatgagtg tgttagggtc gattttaatt gataatgatg cccttcttgc ggtaactgac
      301 tttttaagac cggagcattt ttatgagagc aaaaatgcca aggtttataa agccatgtta
      361 tcacttttcg aaaatcggaa cccaattgat atcattactg tggccgagaa gttaaaagaa
      421 atgaaggaat tgaaaaatat tggcggcaag acatttttag ttaaattagc caatgaagtg
      481 ccaacggcag ctaatgtagc cagttacggt aaaattatta aagctttagc cgctaaaaga
      541 gatttgattt cagcagcagc ccgaattacc gagaaagctt ttgatgagtc tttaccctca
      601 gaacaacttt tagatttagc cgaacaagaa atcttttctc tttcccaacg gcatattaaa
      661 aataccccag tttctttaaa agaggtttta acggctagtt ttgatcgttt agatgagtta
      721 caaaaattag gcagtggttt aagaggagtg ccgactggtt ttacttcttt agacaaaatg
      781 ctagccggaa tgcaaaatag taatttgctt attttagcgg ccagacctgg aattggtaaa
      841 accgcctttg ttttaaatgt cggtcggcac gttgctgttg aagaaaagct gccaacagtt
      901 attttctctt tagaaatgag taaagaagaa ttagttgatc gacttttagt tcggcaaggt
      961 ttaattgacg cttggaaatt aaaaacggga cagttgtcgg atgacgattt tacttctttg
     1021 tctgaagcga tgggggtttt ggcagaagca cctctattta ttgacgatac cccgggttta
     1081 acagtgacag aattaagaac taaatccaga cgattgcaat tagataaagg aattaagttt
     1141 attattgtcg attacctcca gctaatgcat ggtcagacaa aggataatcg ggtccaggag
     1201 gtatcggaaa tatcccaagg actgaaaaat ttagccaggg aattaaaagt gccggtttta
     1261 gcggccgccc aattatcacg ggcaatggaa gctcggggag gccggccgcg gctgtcagat
     1321 ttaagagaat cgggcagtat tgaacaggat gccgatgtag ttatgttttt gcatcgagaa
     1381 gatgaagata ttaaagaaat ggtcactctt tccattgaaa aacaccgtaa tggtcctacc
     1441 ggccaaatga atcttttctt taatggcaaa caggttagct tttttgatgt tgagaagaaa
     1501 taaaagtgcc gaaagaggga atcgaacccc catggtgttg ccaccacacg attttgagtc
     1561 gtgcgcgtct gccaattccg ccatttcggc gatgtaattc agcctaatta tatcagataa
     1621 atctgtttat taaggtatct gctgttataa ttaattatgt attttgtatt ttggttaata
     1681 gctattgttt tatttttata tttatcttgg aaacagttac aggaaggcta taacagccaa
     1741 cagattatcg gatttagttg gggcttggtt ttggcaatga tcttgattgg tcgtttgact
     1801 ggtggagaat ggcagagtga ttcaggatgg ttaattttag ctatttggcg gcctttacga
     1861 gtaaattatt ttgttagttt gattatagct gttttgcttt gtgcttggat gtcgagaaaa
     1921 aataattgga aattatggtc ggtgttggag gatttaaccc cggctttttt ggcctttata
     1981 acttttggtt taatggccga tctttgccgt gcttttactt ggaaattatt aattgacttg
     2041 gctatcgcca ttatggctgt tgtttggagc cgggggttaa agagtcgtta taggtcattg
     2101 gtttggtatc cgtcagggaa gaagggtttt gtgtttttag ccattagtac cattaatttt
     2161 gctctaatat ctatcagtac tttttttcta gaagataggc attttttccc ttatttatat
     2221 ttgggaacta gtttgatttc tggatgggga ttgtatatgc tgggaagaag atgaataata
     2281 acgaactgaa tttacctaaa aaacttttag aaccgattag attattttta gaaagggaaa
     2341 ttgttcgatt gaagcgggct gaaaaaaagt taaaaaaggc cgatcctttc aaagataatc
     2401 aacggactaa tgataattcc cttgaagaag atgtggacga acagattggc cattttgata
     2461 aacaggttaa ggttaaattt ttaaacagac aaatagttca gttaagaaag gcgttaacaa
     2521 tgattaaatt gggtaaatat ggtatttgtg agaaatgcgg caaaatgatt gatactgata
     2581 ggttggcggt taagcccgat actactcttt gtattaagtg tgaacgagaa caagaaaaat
     2641 agcggaaata agatggcaga attgggattg ttattgatca ttattggtgg cggaagaaat
     2701 ttatgggaga ggtggaaatg gggttatgtt aatgattatt ggaatttttt aggaactggt
     2761 atttataata atttaaatga ttggcttatt agtttgggct tgttgattta cttaggggct
     2821 atatggaaga aaaaaccaca ccggaagtaa tttatgaaga ccagaatatt ttggttttag
     2881 ataaaccggc tggttgggtt acaactaagg agaataatca ttcagaacgt aatattgaaa
     2941 gatggctgga aaagatggat aaaaataatt tacctcggca ggggattgtt caccgtcttg
     3001 ataagggaac ctccggtgtt ttgatagtgg ctaaaaatag ccaaacgtta gccgatttta
     3061 aaaagcgatt tcgacaaaga caggttgaaa aaagatattt ggcttggata gaaggggacc
     3121 tgccggctga aggcgatatt aatttaccta ttggaaggtc aaaatataat cgggagaaat
     3181 ttcaagtaag tgaggctggt aaaaaagccc aaactaagtt taaagtttta cgaaaaatta
     3241 atattggcgg aagaatatat agtttagttg atgttaattt aatcactggt cgaacccacc
     3301 agattagggt tcattttagt catcttggct ggccgattgt tggcgatgtg gtgtatggga
     3361 gtaaaaacaa aacagttgat cggccggaat tacattgtta tcagataaaa attgccggtt
     3421 ttactttcga aagtccattt agtgaaaatt tttatgaaaa aacacagtag gagaattaat
     3481 cgaattgcct ggctgatctt tgggggttta attctattgc tcgcattagc ttttttagaa
     3541 aatgatttta agtgggatgg aaaacgagat atgagaattg gcataattac cgacgaaaat
     3601 ttggccgttt tagttatttc tcccgaaaga agaatgatta atcgagtgga aaca
//
LOCUS       DAOOIE010000050         3486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_20886_length_3486_cov_4.253279, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000050 DAOOIE010000000
VERSION     DAOOIE010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20886_length_3486_cov_4.253279"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PK131_01855"
     CDS             <1..214
                     /locus_tag="PK131_01855"
                     /inference="COORDINATES: protein motif:HMM:NF020845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPD44901.1"
                     /translation="YEDQSVGVVLPKSISSKVIKTENAIKGNTATNALKPALVESGLK
                     ILVPLFIKVGDEVMINTETGSYVSKC"
     gene            complement(218..631)
                     /locus_tag="PK131_01860"
     CDS             complement(218..631)
                     /locus_tag="PK131_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44902.1"
                     /translation="MKINKEVATVLAASTILGTVYSLKPKNLIAQSELEVKIADLGKH
                     QEEPETPLKIEDGEKIRIFVISTTGGRFDIYIDGKLDSQRGPDAVFFCGRNDAEMEGT
                     THEFFLKPGEHQVTVYKNLDKNKSDTAEFQIIPKN"
     gene            762..835
                     /locus_tag="PK131_01865"
     tRNA            762..835
                     /locus_tag="PK131_01865"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:796..798,aa:Arg,seq:tct)
     gene            844..915
                     /locus_tag="PK131_01870"
     tRNA            844..915
                     /locus_tag="PK131_01870"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:877..879,aa:Arg,seq:tcg)
     gene            920..1004
                     /locus_tag="PK131_01875"
     tRNA            920..1004
                     /locus_tag="PK131_01875"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:954..956,aa:Leu,seq:tag)
     gene            complement(1191..1724)
                     /locus_tag="PK131_01880"
     CDS             complement(1191..1724)
                     /locus_tag="PK131_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44903.1"
                     /translation="MSKEREKAASSSDIWSKSWLPAYLLQKRDDIRSYLVNDSTFFID
                     PANLLCEAWTSYKNITNNDFYQSLGTKIPELLTKCINDQEIRKTLTSDDGMGGIEGKL
                     WVNYDDDGQYLTFGLFFPKESELKIFSAKIYKNKSVSFYLRDQNRKIVATQDNISNEQ
                     INQILDRLDNVFQDNSA"
     gene            complement(1737..3050)
                     /locus_tag="PK131_01885"
     CDS             complement(1737..3050)
                     /locus_tag="PK131_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44904.1"
                     /translation="MIKNFKFFNCVLGLALFFLSCHPILAKNVYGLHLTQPSDLTTAQ
                     AVINSSGGDWGWATIVIRTDQLDYYTWQNFFDNCRKYHIIPIIRLATTMENNVWLRPS
                     RADIDNLANFLNSLNWPSTPQHIILFNEINHGQEWGGQVDIKNFTDLAIYAVDKFKSL
                     NPNFFILSPGLDLAAPEKMPAYKNFANVYDEIKHYKPEYFSKFDGLASHYYPQNSPKD
                     FNYELKYFPQHIPIFITETGWEHKRPYTCQAAAALTLKIVRDYAKNPKVQAVTPFIYN
                     YPQPPFNNFSWLDNQEQLLPEYKCFIDEPKSANQPYQITNFEFQKIHLPIFMFVQKEY
                     SGQIYLKNTGQSIWGETSFCLQPQASANIQVEPICTDNHRVFPGQIKIFSFKFKVNST
                     NPFKENYLAWEKIKPIEISPLTTNSTIYRPKTDLKERINGFLFKK"
     gene            3091..>3486
                     /locus_tag="PK131_01890"
     CDS             3091..>3486
                     /locus_tag="PK131_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013683914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HPD44905.1"
                     /translation="MDKEEILQAKIKAVEEEIRKTPYDKSTEHHHGVLKAKLARLRDE
                     VDKVDQRKSSKGIGYALKQSGDATVALLGPPSSGKSTLLNYLTKAESLVGSYDFTTLT
                     VIPGMLEYKGVKIQLLDLPGIDSKKTVNKG"
BASE COUNT         1060 a          568 c          772 g         1086 t
ORIGIN      
        1 ttacgaggat caatcagtag gcgttgtttt acccaaatca atttcaagta aggttattaa
       61 aactgaaaat gccattaagg gtaatacggc gactaatgcc ttaaaaccgg cacttgtaga
      121 atcggggttg aagattttgg tacctctttt tattaaagtt ggcgatgaag taatgattaa
      181 taccgagacc ggatcttacg tttccaaatg ttaattttca gtttttgggg attatttgga
      241 attcggcggt atccgatttg tttttgtcga ggtttttgta gacagtaact tggtgttcac
      301 cgggtttaag gaagaattcg tgggtagtcc cttccatctc ggcgtcgttg cggccgcaga
      361 aaaagacggc atcaggccct cgctggctat ccagcttacc gtcaatgtaa atgtcgaagc
      421 ggccgccagt agttgagatg acaaaaatcc gtatcttttc gccatcttct attttgagag
      481 gggtttctgg ctcttcttga tgctttccta ggtcggctat tttaacttct agctcggatt
      541 gggcaattag atttttgggt tttagagagt aaacagttcc tagaatcgtc gaagctgcta
      601 aaacagtggc tacttccttg tttattttca taaaaaataa aatttgctaa tttgggggag
      661 tgaaggatta tagcatagaa gataaaattt tcctaaagct ggtaaaatgt agacattgag
      721 atccgccggc aggcaggttg aaaattgaaa attaacatgc tgtccccata gctcagttgg
      781 atagagcgtc ggccttctaa gccgaagatc acaggttcga ttcctgttgg ggatactgaa
      841 attgcgccta tagctcaacg gatagagtgc tagtcttcgg aactagtgat gggggtccga
      901 ttccctctag gcgcacccag ccgaagtgat ggaactggta gacatatacg gcttaggacc
      961 gtatggggca acccgtgggg gttcaactcc ctccttcggc accaagagga tttagttctg
     1021 tccgaaagtg ctgaaaaaga atataaaaaa ttattagcaa ttgctaaaga tccatttgac
     1081 agtaaaaaac ttatcggata ttgtttgaga tcaatgataa agaaaagagg attggggttc
     1141 taaaaactgc acatagacgg agtttgtaag ttttgaattt cgaattctgc tcaagctgag
     1201 ttatcttgaa acacgttgtc gagccggtct aaaatttgat taatttgctc attgctgatg
     1261 ttgtcttgag tagctacaat ttttctattt tgatccctta agtaaaaact aactgattta
     1321 tttttgtaaa ttttagccga aaaaatcttt aattcacttt ctttaggaaa aaataagccg
     1381 aaagttagat attggccatc gtcgtcataa tttacccaca atttgccttc aattcctccc
     1441 ataccatcat cactggtaag tgtttttcta atttcttggt catttataca tttggtgagt
     1501 aattccggaa tttttgttcc caaagattgg taaaaatcat tattggtaat gtttttgtag
     1561 cttgtccaag cctcacacaa aagattggcg ggatctataa agaaggtgga gtcattaact
     1621 aaataactgc gaatatcatc acgtttttga agtagataag ccggtagcca actttttgac
     1681 cagatatcag acgatgacgc tgctttttct ctttctttcg acatatcttg attatatcat
     1741 tttttaaaaa gaaaaccgtt aattctttct tttaagtcgg ttttaggacg gtaaatagtg
     1801 ctgttagttg ttaaagggct gatttctatg ggtttaattt tttcccaggc aagatagttt
     1861 tctttgaaag gattagtaga atttacttta aatttaaagc tgaaaatttt tatttggccg
     1921 ggaaaaactc gatgattgtc ggtacaaatt ggctcaactt ggatattggc actggcttgg
     1981 ggctgaagac aaaatgaagt ttcaccccaa atcgattggc cggtattttt caaataaatt
     2041 tgaccagaat attctttttg aacgaacatg aaaatcggca agtgaatttt ttgaaattcg
     2101 aaattggtga tctgataggg ctgattggct gatttcggtt catctataaa acatttgtat
     2161 tctggtagaa gttgttcttg attatccagc caagaaaaat tattaaaagg cggttgggga
     2221 taattataaa taaaaggggt tacagcctga actttgggat ttttagcata atcacgaaca
     2281 atttttaggg ttaaagccgc ggcagcttgg caggtatagg gccgcttgtg ctcccaaccg
     2341 gtttcggtga taaaaatggg aatatgttgg ggaaaatatt ttagttcata gttaaagtct
     2401 ttaggactgt tttgggggta atagtggctg gccaagccat caaatttact gaagtattct
     2461 ggtttataat gtttaatttc atcgtagaca ttggcaaaat ttttataagc cggcattttt
     2521 tccggggcag ccagatctaa tcccggagag agaataaaaa aattgggatt gagtgattta
     2581 aatttatcaa cggcataaat tgccaaatcg gtaaaatttt taatatccac ttgtccaccc
     2641 cactcttgac catgattaat ttcattaaaa agaataatat gttggggagt tgacggccag
     2701 ttgagagaat tgagaaagtt ggcgagatta tcaatatcgg cccgagaggg tcgaagccag
     2761 acattatttt ccatagtggt agccaggcgg attatgggaa taatgtggta ttttcggcaa
     2821 ttatcgaaaa aattctgcca agtataataa tcgagctgat cagttctaat aacaatggtt
     2881 gcccaacccc aatcgccccc cgaagaatta ataaccgcct gagctgtggt aagatcgctc
     2941 ggttgagtta agtgaagacc gtaaacattt tttgctaaaa taggatgaca ggagaggaaa
     3001 aaaagggcta aacccaaaac gcagttgaaa aatttaaagt ttttaatcaa ttcgtaattt
     3061 gtaattttta attattaatt attatagcgg atggataagg aagaaatatt acaagcaaaa
     3121 attaaagcag tcgaggagga aattcgtaag acaccctacg acaagtcgac cgagcatcat
     3181 catggggttt taaaggccaa actggctaga ttgagggatg aggtggataa agtagatcaa
     3241 aggaaaagca gtaagggaat tggttatgct cttaagcaat caggcgatgc taccgtagct
     3301 ttattggggc cgccaagcag cggtaaatct actttattaa attacctgac taaagctgaa
     3361 tccctagttg ggtcgtatga ttttactact ttaaccgtaa ttcccggaat gttagagtat
     3421 aagggagtaa aaattcaatt attggattta ccgggaatag acagcaaaaa gacagtaaat
     3481 aagggt
//
LOCUS       DAOOIE010000051         3395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_21576_length_3395_cov_3.817365, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000051 DAOOIE010000000
VERSION     DAOOIE010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21576_length_3395_cov_3.817365"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..1024)
                     /locus_tag="PK131_01895"
     CDS             complement(116..1024)
                     /locus_tag="PK131_01895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPD44906.1"
                     /translation="MKFSIIIPNYNGAALLEDCIKSLGLSNELIIIDNGSKDNSLKKI
                     NELKHSLKIKNCKLIINQTNYGFAAAVNQGIKAAKYNYVVIANNDLVIDKNWFKYISQ
                     AIKKYPKVACFCGIVLNKSGTKYESAGLKFFYSGKCQNLKNGEKFIRADTPVSPYYVW
                     GSSAALVVYQKSVLEKIGFFDEDFFAYEEDVDLALRLDKFGYKTLLVPQAISYHLGGG
                     TSSKMGNLRQINDFSNWIFIIIKNFSFKEIFLNFPQIIYQRLINFIVIPHKSIKIFRR
                     LKLIPKMLKKRQELQNLLKSSHDHWY"
     gene            complement(993..1433)
                     /locus_tag="PK131_01900"
     CDS             complement(993..1433)
                     /locus_tag="PK131_01900"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HPD44907.1"
                     /translation="MKKKLLIFILLFSIILSVMTVFSRKNTHQLIINGRNYQIEYALT
                     EAQITKGLSGRTSLCSNCGMLFVFNNEGILPFWMKDTLIPLDIIWIDGSGKIVHYVNA
                     TTTNSLETYQNSIPARYVLELNSGDFNKLGLKIGDEILNYHSQL"
     gene            1461..2354
                     /locus_tag="PK131_01905"
     CDS             1461..2354
                     /locus_tag="PK131_01905"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPD44908.1"
                     /translation="MKKVWWKILLSLFLIYLVWIRVDFRAVGKELAQAPWWLAIALVA
                     YLFLTNFLAGRRWSLVLLGNEAKWADTWNFVKSNYIGSFYGLVFPTAVAGDLLKWLPL
                     ISKYKKISKAKIGLSVLTDRIIGGMGFLIVAFLVAVAGYFFKIPFPIYLLWLFSFGLG
                     AMIIFLIIINFIHFEKYLWGRKLTSLIECLRKENHRVIIRGLILSIVGQILWAVPTWV
                     CLRVFGTTISFWSAMIILPIISAVLVLPISVAGFGAREQLFLFFFTQMGGQPEKVVAA
                     SAFIGIIGVLNSLIGGLLMLK"
     gene            2359..3330
                     /gene="galE"
                     /locus_tag="PK131_01910"
     CDS             2359..3330
                     /gene="galE"
                     /locus_tag="PK131_01910"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008511934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HPD44909.1"
                     /translation="MILIVGGAGYIGSHVNKELTRQGYETLVLDDLSSGKEILVKWGK
                     LIKGNLGDEALLAKIFSENKIEAVMHFAAFKAAGESMIEPEKYYLNNVGATLRLLKVM
                     RQYGINKFIFSSSAAVYGEPQYIPIDEKHPTNPINTYGTTKLMVEKILNDYERAYGFL
                     SVRLRYFNAVGADPEAEVGEWPGSSANLVPIALEVAMGRKAELTIFGTNYPTPDGTCI
                     RDYIHVTDLAAAHVAALKFLDREKKSEVFNLGNGKGFSVREVVAMTKKVTGIDYPVVE
                     GDRRPGDPPILVADSQKAVAVLGWQPVFDSLKVIVETAWQWHRQAKV"
BASE COUNT         1034 a          502 c          706 g         1153 t
ORIGIN      
        1 ccggcgggag gagaaaaaaa atttatattg gttggaatgg tcgattttta ctaaatgccg
       61 gactaaattc agggtataat tggaaacacc ggtgccataa ggaacggccg aaacgtcaat
      121 accaatgatc atgagatgat tttagcaaat tttgcagttc ttggcgtttt ttaagcattt
      181 tggggattaa ttttaatcgc ctgaaaattt taattgattt atggggaata actataaaat
      241 ttattaatct ttgataaata atttgtggaa aattaaggaa aatttcttta aaagaaaaat
      301 ttttgataat aataaaaatc cagttgctga aatcattgat ttgtcttaaa ttacccattt
      361 ttgaactagt accaccgcca agatgataac taattgcttg agggaccaat aaagttttat
      421 aaccaaattt atctaatctt aaggcaagat caacatcttc ctcgtaggca aaaaagtctt
      481 catcaaaaaa accaattttt tctaaaaccg atttttggta gacgaccaaa gcggccgagg
      541 aaccccagac gtagtagggg ctgaccggtg tatctgcccg aataaatttt tcaccgtttt
      601 taaggttctg acatttaccg gaatagaaga atttcaagcc ggcactttca tatttagttc
      661 cagatttatt taaaacaatg ccacaaaaac aagcgacctt agggtatttt ttaattgcct
      721 ggctgatgta tttgaaccaa tttttatcaa tgaccaaatc attgttagca ataaccacgt
      781 agttatactt agcggctttg atgccttggt tgacagcggc agcaaatcca taattagttt
      841 gattaataat taatttgcaa tttttaattt tcagagaatg ttttaattca ttaatttttt
      901 taagactatt atcttttgag ccgttgtcga tgataattaa ttcattggat aaacccaatg
      961 atttaatgca gtcttctaaa agagcagccc cgttataatt gggaatgata attgagaatt
     1021 tcatcgccga tttttaaacc taatttatta aaatcacccg aattaagctc caaaacatac
     1081 ctagccggaa tagaattttg ataggtttca agggaattag tggtggtggc gttaacgtaa
     1141 tgaactattt tgccgctacc gtcaatccag attatatcaa gaggaatcag agtatctttc
     1201 atccaaaatg gtaaaattcc ttcattattg aaaacaaaaa gcatgccgca gttagagcaa
     1261 agagaagttc gaccagaaag tcctttggta atttgggcct cagttagggc atattctatt
     1321 tgataattcc tgccattaat gattaattga tgagtatttt ttcgggaaaa aacggtcatg
     1381 actgataaga taatcgagaa tagcaagata aagatcaaaa gttttttttt cacttctctt
     1441 agtataatat gaatggaaag atgaaaaaag tttggtggaa aattctctta agtctttttt
     1501 tgatttatct tgtttggatt agggtagatt ttagagcagt ggggaaagaa ttagcccaag
     1561 ctccatggtg gctggcaatt gccctagtcg cctatctttt tttaaccaat tttttagccg
     1621 gccggcgttg gagtttggtt ttattgggta atgaggccaa atgggctgat acttggaatt
     1681 ttgttaaatc gaattatatt ggctcttttt acggattggt ttttccgact gctgttgccg
     1741 gtgatcttct taaatggctg cccttaattt ctaaatacaa aaagattagc aaagccaaaa
     1801 tcggcctttc ggttttaact gatagaatta tcggcggcat gggatttctt atcgttgcct
     1861 ttttagtagc tgttgccggt tattttttta aaattccgtt ccctatctat cttttatggc
     1921 tttttagttt tggtttagga gcaatgatta tttttttaat tataattaat tttattcatt
     1981 ttgaaaaata tttgtgggga aggaaattaa cctctctaat tgagtgtttg aggaaggaga
     2041 atcatcgggt aattattcgt ggtttaattt tgtctattgt tggccaaatt ctatgggcag
     2101 ttcccacttg ggtttgtctc cgggtttttg gaacaaccat tagtttttgg tctgccatga
     2161 taattcttcc tataatatcg gcagttttag ttttacctat ttcggtggct ggttttggcg
     2221 cgagagaaca attgtttttg ttttttttca cccaaatggg aggacagccg gaaaaggttg
     2281 ttgccgccag tgcttttatt gggattattg gagtcttaaa ttcattgatt ggtggtttat
     2341 taatgttaaa atagatcgat gattttaatt gttggcgggg ctggctacat tggttcccac
     2401 gttaataaag aattaacccg acaaggttat gaaactttag tcttggatga tttatcgtca
     2461 ggaaaagaga tcttggttaa gtggggtaag ttaatcaaag gcaatttggg agatgaagca
     2521 ttattagcta aaatattttc tgagaataaa attgaagccg taatgcattt tgcggctttt
     2581 aaagccgccg gtgagtcgat gattgagcca gaaaaatatt atttaaataa tgttggtgct
     2641 accctgagat tattaaaagt tatgagacag tatggaatta ataagtttat tttttcttca
     2701 tcggcagcag tttatggtga gccgcagtat attccaattg atgaaaaaca tccaactaat
     2761 ccaattaata cctatggaac aactaaatta atggtggaaa aaattctcaa cgattatgaa
     2821 agagcttatg gttttttgtc cgttagattg agatatttta acgctgttgg ggctgatcct
     2881 gaagctgaag ttggtgaatg gcccggcagc agtgctaatt tagtaccgat agctttagaa
     2941 gtagccatgg gtagaaaagc agaattgact atttttggta ctaattaccc taccccagat
     3001 ggtacctgta ttcgggatta catccacgtg actgatttag ctgcggctca tgtcgccgcc
     3061 ttgaaatttt tagatagaga aaaaaagagt gaagttttta atttggggaa tggcaagggg
     3121 ttttcggtaa gggaagtagt ggctatgact aagaaagtaa ccggtattga ttatccggtg
     3181 gttgaaggag acagacgtcc gggtgatccc ccaattttag tggctgatag tcaaaaagca
     3241 gtggccgttt tgggttggca gccggttttc gatagtttaa aggtgattgt agaaacggct
     3301 tggcaatggc atcgccaggc taaagtataa attaaactta taacaaataa ctataagata
     3361 tattaaaatt tatgaaataa ataattaatt atgat
//
LOCUS       DAOOIE010000052         3392 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_21604_length_3392_cov_2.914594, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000052 DAOOIE010000000
VERSION     DAOOIE010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21604_length_3392_cov_2.914594"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="PK131_01915"
     CDS             <1..683
                     /locus_tag="PK131_01915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD44910.1"
                     /translation="ANNQGLKIAQGNYILFLNSDTIVLHSSISQALDWLCSHPEAAAC
                     TAQLLNKDKTIQASGGFFPNLANMLTWSLGLDDLPGINYLIKPFHPHPPKFYTHDSFY
                     LRDHQQDWITGAFMLIRRELLDKIGGFDENYFMYGEEVELAYRLKQITTNNQIWYLVG
                     PQIIHFGRASAADQSQPILNEYRGVLAFFKKHKPYYQFVIAQFLIKTNAWLRSLIRPV
                     YRRVYQQL"
     gene            680..2053
                     /locus_tag="PK131_01920"
     CDS             680..2053
                     /locus_tag="PK131_01920"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPD44911.1"
                     /translation="MNILRPILAILIIFIPLYPKFPLWGVADTYVAIRLDDLVVAIAV
                     FIWLLYQIKHHFPILKEKITWLVLAYLAAILLSTLYAIFIFQVVSSHLLILNFLRRIE
                     YISLMFITISAVQNKKDLNFTYISLIITVLLVSIYGFGQKYLHFPVISTMNSEFSKGQ
                     LLQMDVWTRISSTFAGHYDLAAYLTLVLIIIGSLFLISRSKSSKYISLITWLIGFSLL
                     NLTASRISTFAFWGGMVLALIFIKKYLWIIPFSILMIFSIINSKDLNRRLVATLNYLP
                     QHFPSLPQQSPLTNTLTPTPVSSPTVTQSTPTTILSSKPVPTVFHHLPAIEEFPSIDT
                     DAGVARSGEIRFNAEWPRAINAFKRNIFLGSGPGSITLATDNDYLRLLGESGLLGLIT
                     FLIIFLYFIYQTIKKPSHLAFIFLACLIATGANALFIDVWEASKFAYTFWIMMGIYYQ
                     VLKFNND"
     gene            2046..>3392
                     /locus_tag="PK131_01925"
     CDS             2046..>3392
                     /locus_tag="PK131_01925"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HPD44912.1"
                     /translation="MTKKILILVTLILGLIVRLYKIDSPIADWHSWRQADTASVTKNF
                     INHTGTFFIPTYHDLSNIPSGQENPHGYRMVELPIYNLIAAGLSKFCSVEIASRLVSI
                     FFSLGSALLVYFITISWTKNFKIGFFAFSIFLFLPFNIYYSRTILPEPTAVFFMLLSL
                     YLFTTNFYLSAIALAISVLIKPYTALLLFPIFLFLSWHKKITAKFAFCLIAFLPLFFW
                     RLWIKNFPAGIPASAWLFNGGKMRFRPVWFRWLFYERIGKLIMGVLGVIPLYLGLAFR
                     KNKNRSLCFFLFLGILTYFSVIARGNIQHDYYQILIIPFIAIITGIGLNYMFKFSSFL
                     GTIIVIIFALAFSWYQIRDYYIIDPAIVEAGQAAQQLLPKDAWVIAPRNGDTAFLYQT
                     NHAGWPIEIYDLETLKSRYPEKKFYLVTVNKNSYTTELKEKYSVIIDNNNYTIVDLN"
BASE COUNT          898 a          732 c          518 g         1244 t
ORIGIN      
        1 aagccaataa tcaaggttta aaaatagccc agggtaatta catcttattt ttaaattccg
       61 acacaatcgt tctacactcc tccatttctc aagctcttga ctggctttgc tcccatccag
      121 aggcagccgc ctgcactgct caacttttaa ataaagataa aaccattcaa gcttctggcg
      181 gtttttttcc aaatttagcg aatatgttaa cctggtcgct aggtcttgat gatttgccgg
      241 gaatcaacta cctaatcaag ccttttcatc ctcatcctcc aaaattttat acccatgatt
      301 ccttttatct acgagatcat caacaagatt ggattaccgg cgctttcatg ctaattcgcc
      361 gggaattact tgataagatt ggtggtttcg atgaaaatta ttttatgtat ggtgaggaag
      421 ttgagctggc ttatcgctta aagcaaatta ccaccaacaa tcaaatttgg tatttagtcg
      481 gccctcaaat aattcatttt ggtcgagcgt cggcggctga tcaatctcaa ccaattttaa
      541 atgaatatcg aggtgtttta gccttcttta aaaaacacaa gccttactat caatttgtca
      601 tagctcagtt cctgattaaa accaacgctt ggttaagaag tttgatcagg ccggtttatc
      661 gccgtgttta tcaacaatta tgaatatttt aaggccaatt ttagcaattc tgatcatttt
      721 tattcctctt tatcccaaat ttcccttatg gggagtggct gatacttacg tggctattcg
      781 tttagacgat ttagtcgtgg caatagctgt tttcatctgg cttctttacc aaattaaaca
      841 tcattttcca attctaaaag aaaaaataac ttggcttgtt ttggcttact tagcggctat
      901 tttgctcagc accctttacg ccatttttat tttccaggta gtttcatctc atcttcttat
      961 tttaaatttt ctccgtcgaa ttgaatacat atctcttatg tttattacta tttccgctgt
     1021 tcaaaataaa aaggatttaa atttcactta tatttctctc atcattaccg tcctactggt
     1081 ttctatttac ggatttggtc aaaaatatct tcattttcct gttatctcaa ctatgaattc
     1141 agaattttct aaaggacaac tccttcagat ggatgtttgg acaaggatta gctccacttt
     1201 tgccggccat tatgatttag ccgcttactt aactctagtt ctgattatta ttggcagttt
     1261 atttttaatt agccgatcaa aatcctcaaa atacattagt ttaattacct ggttgatcgg
     1321 ctttagtctt cttaatttaa ctgcttctcg aatttccact tttgcatttt ggggaggaat
     1381 ggttttagcc ttgattttca tcaaaaagta tctttggatt attccttttt caatcttaat
     1441 gatttttagc attatcaact ccaaagatct taatcgacgg ctcgtggcaa ccttgaacta
     1501 tctgccccaa cactttcctt ctcttcctca acaatcacca ttaaccaaca ctcttactcc
     1561 tacccctgtc tctagtccaa ccgtaaccca atcaactcca accacaattc tctcttccaa
     1621 acctgtccct actgtctttc atcatctacc ggcaatagaa gaatttccat caattgatac
     1681 tgatgccgga gtagcccgat cgggcgaaat tagattcaat gctgagtggc cacgggcgat
     1741 taatgccttc aaacgcaata tttttcttgg cagcggtcca ggctcaatta ccttagccac
     1801 tgataacgat tatctacgtc ttctgggtga aagtggtctt ttgggtttaa ttaccttctt
     1861 aattatcttc ctctatttta tctatcaaac aattaaaaag ccgagtcatt tggcatttat
     1921 ttttctcgct tgtttaatag ccactggagc caacgccctt tttattgatg tttgggaagc
     1981 ttctaagttt gcttacacct tttggattat gatgggtatt tactatcagg ttttaaaatt
     2041 taataatgac taaaaaaatt ttaattctgg tgactttaat tttgggactt attgtccgcc
     2101 tttataaaat tgacagccct atagctgact ggcattcctg gcgccaggcc gacactgcct
     2161 cggtcaccaa aaattttatt aatcacactg gtactttttt cattcccact taccatgacc
     2221 tatctaatat cccttctggt caagaaaatc cccatggcta ccgaatggta gaattaccaa
     2281 tttataattt gattgcggcg ggtttatcca aattttgctc tgtcgaaatc gcctctcgat
     2341 tagtttccat cttcttctct cttggcagtg cccttctagt ttattttatt actatcagtt
     2401 ggactaagaa ctttaaaatc ggtttcttcg cttttagcat ttttctcttt ctccctttta
     2461 atatctacta ttctcgaaca attctgcctg aaccaacagc agtttttttt atgctcctct
     2521 ctctctattt atttacaacc aacttttatc ttagtgccat tgctttagct atttcggttt
     2581 taattaaacc ttataccgct cttcttcttt tccctatttt tttatttctg tcctggcata
     2641 aaaaaataac tgctaaattt gccttctgct taattgcctt cttaccccta tttttctggc
     2701 gcctctggat aaaaaacttt cccgccggta ttcctgcttc ggcttggctt tttaatggcg
     2761 gcaagatgcg ctttcgacca gtctggttcc gttggttatt ttatgaaaga ataggcaaat
     2821 taattatggg agtcttaggg gttatccctc tttatttggg tttggctttc cgaaaaaaca
     2881 aaaatcgatc actttgcttt tttcttttcc tcggcatttt aacttatttt agtgtcattg
     2941 ctagaggaaa tatccaacat gactattacc aaattttaat catccccttt atcgccataa
     3001 tcaccggtat tgggctaaat tatatgttta aattctcgtc atttttgggc accattatcg
     3061 tcattatttt tgccttggct ttttcctggt accaaataag agattattat attattgacc
     3121 cagctatagt tgaggctggc caagccgccc aacaacttct tccgaaagat gcttgggtta
     3181 ttgctccacg taatggtgat actgcttttc tttaccaaac gaatcatgcc ggctggccaa
     3241 tcgaaatcta tgatttagaa actcttaaga gtcgttaccc tgaaaaaaag ttctatttag
     3301 taactgttaa taaaaacagt tatactaccg aattaaaaga aaaatattcc gttataatag
     3361 acaataataa ctatactata gtagatttaa at
//
LOCUS       DAOOIE010000053         3325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_22186_length_3325_cov_4.099083, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000053 DAOOIE010000000
VERSION     DAOOIE010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22186_length_3325_cov_4.099083"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1268)
                     /locus_tag="PK131_01930"
     CDS             complement(93..1268)
                     /locus_tag="PK131_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPD44913.1"
                     /translation="MNNFVPLAVTLRPLTLADFIGQEHLVGPGKPLRLVIENKGLSAG
                     RQGLFSMIFWGPPGVGKTTLARIIAHESGMEIYELSAVSAGKDEIKKIVKNKTPLYPK
                     ILFLDEIHRFNKAQQDYLLPFVETGELILIGATTENPSFEIIPPLLSRCRVFIFNELN
                     EKDLNKIIDRIEKKLFGDKGDLIDKPARDWLINMANGDARQLITIAENTFTFYHQINV
                     RTLMAALQNKFLRFDKKGEEYYNLISAFIKSMRAGDVDAALYYLARMVDAGQDPLYIA
                     RRMVVFASEDVASPTALIVANAVFQACQQIGYPECQENLAAGTVYLSLAKKDRRAYDA
                     YMKALTDVKQYGNLPIPLKIRNPVTKLMKDLGYGRGYEKYDKTSYLPDKLKGKRYFK"
     gene            complement(1265..2440)
                     /locus_tag="PK131_01935"
     CDS             complement(1265..2440)
                     /locus_tag="PK131_01935"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPD44914.1"
                     /translation="MTALLAKINQRKAFIVIFTIVLTDLIGFGLIIPIIPKLSQDFNI
                     IGPKLGLLLAAYPLAQFFAAPYLGSLSDKYGRRPVLLISKLGTVIAYLIFAYAQAYWL
                     ILLSRFIDGFTGGNLPVARAYMSDITTDRNRPRGMALIGAAYGLGFIIGPVVGGFMFG
                     ISGGKTLPALTGASLCFLSFIFTWLFLKESPSHHLVSAPVSFNPITNYKQVTNFPAVK
                     RMITIQFLFMTCLYALQSVLPIFAANRYHLTPQTIGFLMVYIGLINIVIQLMIVKFSS
                     RNLSRSVKMGLLFTGLGIFLITLVPSYWFIFPMAIVISLGIGFLNAYYPSLLLSISGS
                     MIKGEVTGVYESITSITQVIGPAIAGSLVVHFPYSVCYSTALIITLSAFLIDYQFHS"
     gene            2397..>3325
                     /locus_tag="PK131_01940"
     CDS             2397..>3325
                     /locus_tag="PK131_01940"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPD44915.1"
                     /translation="MKAFLWLILASSAVIKSIGLNQSLWLDEAITANTVHHYTLANIL
                     KQFSVTDFHPPLYYFFLKVWTNIFGYSEIALRLPSVIFSLITVYVVFLIIRKIWPDQS
                     RASRAALWGAALIAFHPLLIYYAQEARMYSLVVMLIAFAIYAKIKKSRWEFYLWSFLS
                     LSTFYGSAFILITIDFWNWGLWLGLLLLSPLIWKQYSTSQTLIMEVNNWSVALGRINW
                     KNLALIPIKLITGRIPFQIWQGLIVIFFWAAAFKAGLKHKFWLKLLIIPIIIGILVSF
                     KAPMMQYFRFQYLVIPLIILLILFQKTQIVFLL"
BASE COUNT         1103 a          659 c          577 g          986 t
ORIGIN      
        1 tgacttgact cccatcccgg ccggaccagc aattttcact gtatacacct gacgggacat
       61 gtcatgatta tatctaaaaa aatgctaaaa aattatttaa aatacctttt acctttgagt
      121 ttatcgggca gatagctggt tttgtcatat ttttcatacc ctcggccata accaagatct
      181 ttcattaatt tcgtcactgg attacgaatc tttaaaggaa tcggtaaatt accatattgt
      241 ttaacatcgg tcaaagcctt catataagca tcgtaagccc ttctatcttt tttagccaat
      301 gataaataaa cggttcctgc cgccaagttt tcctgacatt cgggatagcc aatttgctgg
      361 caggcttgaa aaactgcatt agccactatc aaagccgtgg gagaggctac atcttctgaa
      421 gcaaaaacaa ccattcttct ggcaatatat aaaggatcct gaccagcatc aaccattcgg
      481 gctaaataat acaaagcagc atcgacatct ccggcccgca tggatttgat aaaagctgaa
      541 ataagattgt aatattcctc ccctttctta tcaaaacgaa gaaatttatt ttgtagggcc
      601 gccattaaag ttcggacatt aatctgatga taaaaagtaa aagtattctc agcgatagta
      661 attaattgac gagcgtcacc attggccata ttgatcagcc aatcacgagc cggtttatca
      721 attaaatctc ccttatctcc gaataatttt ttttctatcc ggtcaataat tttattaaga
      781 tccttttcgt ttaactcatt aaaaataaac acccggcaac gagacaaaag cggtggaata
      841 atttcaaaac tgggattttc agtagtggcc ccgattaaaa ttaactcccc ggtttcaaca
      901 aaaggaagaa gataatcttg ctgggcttta ttaaaacgat gaatttcgtc caaaaataaa
      961 atctttgggt agagcggagt tttattttta acaattttct tgatttcgtc tttaccggct
     1021 gaaactgccg ataattcata aatttccatt cccgattcgt gagctataat ccgggctaaa
     1081 gtagtttttc ctactccggg tggaccccaa aaaatcatcg agaacaggcc ttgcctgccg
     1141 gcagacaggc ctttattttc aatcactaac cgaagaggtt tgcccggccc aactaaatgc
     1201 tcttgaccaa taaaatcagc caaagttaag ggtcttaagg taactgctag gggcacaaaa
     1261 ttattcatga gtgaaattga tagtcaatta aaaaagcaga taaagtaatg attaaagcag
     1321 tgctataaca aaccgaataa ggaaaatgga ctaccaaact accggcgatt gctggaccaa
     1381 ttacttgagt aatactggta atactttcat aaacaccagt aacctcccct ttaatcatcg
     1441 atccactaat acttaaaaga aggctgggat aataagcatt caaaaaacca attcctaaag
     1501 aaattacgat tgccatggga aaaataaacc aataactggg aactaaagta attaaaaaaa
     1561 tacctaggcc ggtaaataat aagcccattt taaccgatcg agaaagattc cgactactaa
     1621 atttaacaat cattaactga ataacaatat tgatcagacc aatataaacc attaaaaaac
     1681 caatggtctg cggggtaaga tggtaacgat tagcagcaaa aataggtaaa actgattgaa
     1741 gagcataaag acaggtcata aaaagaaatt ggattgttat catgcgttta actgccggaa
     1801 aatttgttac ctgtttataa ttggtaattg gattaaaaga aactggcgct gaaaccaaat
     1861 ggtgactggg tgattccttt aaaaaaagcc aggtaaaaat aaaggataga aaacaaagac
     1921 tagcgccagt caaagctggt agagttttac cgccgctgat accaaacata aaaccgccaa
     1981 ccaccggccc gataataaaa cctaaaccgt aagccgctcc aattagagcc atcccccgtg
     2041 gtcgattccg atcggtagta atatcactca tataagcccg agcgacgggt aaattaccgc
     2101 cggtgaatcc atcgataaaa cgagataaca aaatcaacca ataggcttga gcataggcaa
     2161 aaattaaata agcaataaca gttcctaatt tacttattaa taaaaccggt cggcggccat
     2221 atttatcgga taaactaccc aaataaggcg cggcaaaaaa ttgagccaaa ggataagccg
     2281 ccagcaacaa acctaattta ggaccaataa tattaaaatc ttggcttaac ttcggtataa
     2341 tcggaataat caaaccgaaa ccaataagat cggtcaaaac tatggtaaaa ataactatga
     2401 aagctttcct ctggttaatt ttagctagca gcgcggtcat aaaaagcatt ggtttaaatc
     2461 aaagcctgtg gttagatgag gctattaccg ccaatactgt tcatcattat acgctggcca
     2521 atattctcaa gcaattttcc gttaccgatt ttcatccccc tctatattat ttttttctaa
     2581 aagtctggac taatatattc ggttatagtg aaattgcttt aagactaccg tcggttatct
     2641 tctccttaat aacggtctat gttgtttttt taattattag gaaaatctgg cctgaccaga
     2701 gtcgggcaag tcgggctgct ctgtgggggg cggccctaat cgccttccat ccccttttaa
     2761 tttattacgc tcaagaagcc cgaatgtatt ccctagtagt tatgttaatt gcttttgcta
     2821 tttacgccaa aattaaaaaa tctagatggg aattttactt atggagcttt ctcagcctta
     2881 gtaccttcta tggttcggct tttattttaa ttactattga tttttggaat tggggattat
     2941 ggttaggact gcttcttctc tctcctctta tttggaaaca atattctacc tctcaaaccc
     3001 tcataatgga ggtgaataat tggtcagttg ctttaggcag aattaactgg aaaaatttag
     3061 ctcttattcc tataaaatta attaccggta gaattccttt tcaaatctgg caaggattaa
     3121 ttgtcatctt tttttgggca gcagctttta aggcaggcct aaaacataaa ttctggttaa
     3181 aattactaat tattccgata atcattggga tattggtttc ttttaaagcg ccgatgatgc
     3241 aatattttcg ttttcaatat ttagttattc ccttaattat tctcctgatt ttatttcaaa
     3301 aaactcaaat agttttcctg ctttt
//
LOCUS       DAOOIE010000054         3311 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_22317_length_3311_cov_2.760442, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000054 DAOOIE010000000
VERSION     DAOOIE010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22317_length_3311_cov_2.760442"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..802)
                     /gene="map"
                     /locus_tag="PK131_01945"
     CDS             complement(44..802)
                     /gene="map"
                     /locus_tag="PK131_01945"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018282457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPD44916.1"
                     /translation="MVIIKNQKEIGAIRQSCRLAAETLKWVGSLIRPGISTLYLNDNA
                     HHYLVEHGGVPAPLHYNGYPKSICTSVNDVVCHGIPSDKTILKNGDIINIDITAILDG
                     YYGDVSASYPVGQISPKAEKLLKVVKDALDKSIKALKPGKYLNECVGQVIEPYVKKFG
                     FSVVRELGGHGVGLQFHEEPFVYHFDIKRKDILLEPGMIFTIEPMVNCSRDWRIVLNS
                     KDHWTVTTRDGSLSAQFEHTILITPTGHEVLTAI"
     gene            833..1570
                     /locus_tag="PK131_01950"
     CDS             833..1570
                     /locus_tag="PK131_01950"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011175596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPD44917.1"
                     /translation="MKTSWQQVAPWYKKIVGKKGHYYHQRVIMPQVVRLMNLKGGEAV
                     IDLACGNGVLKRNLKKKIKYVGVDLSKNLIAEAQRIDHNSQYLVADVSRELKIKNKFD
                     WVVIILALQNIKNFQGVIRNASQLLKTGGKLLIVLNHPCFRIPRQSSWEIDNSNKIEY
                     RRINRYLSPLAIPIKINGHFTWSFHHCLSDYFQALKENGFGVETLEEWISDKDSQGNR
                     KKMENQARKEFPLFMAIVAYRRLNNSD"
     gene            complement(1527..2156)
                     /locus_tag="PK131_01955"
     CDS             complement(1527..2156)
                     /locus_tag="PK131_01955"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPD44918.1"
                     /translation="MNVANDYRTGIVPTWCPGCPNFLVFAQIQQVLAEKKIQPYQLVF
                     CYDIGCSGNLADFLKCFGFHTLHGRAVAVASGIKRVKPDLTVAVYGGDGGIYGEGLNH
                     TLAAARANIGIKVLVANNFLYSLTTGQTSPTTPFQAKTKSTPLGNPNKPIDPVKLIKA
                     ANPDVWVKTVSATDPAALKTAIGDLFDQPGFALLDISQNCLAFGKQLLP"
     gene            complement(2140..>3311)
                     /locus_tag="PK131_01960"
     CDS             complement(2140..>3311)
                     /locus_tag="PK131_01960"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44919.1"
                     /translation="IIKAHFGKYAEINWTAFQIGFDFAVRNFNQSKIKVKMPDKSVKL
                     SHNYNDGNEAAGWGFLKAGGDFYTAYPMTPASGILHFLAAHQGDYHLQVVHPEDEIAA
                     INLAAGAAYAGARAATGTSGGGFALMTEAVSLLGMAGIGAVVYLISRPGPATGLPTWT
                     SQGDLLFAIHAGHGEFPKIVLSPGSQQESFELSRLAINLAVKHQLPVIFLSDKMLGES
                     AADTTDLSAEKTIMTPLPRILPGTKGQEYLVNSYEHDDQGFSTEDAEVTKKMVEKRLA
                     KIKNVLPDLPSSTTLRSVSGSTKLIITWGSVTGAVREADLEDYDILPIKTLWPIDPQL
                     ETVINSYQSVVVVENNASSQLTTLLKSQFNFKPDRIITKYDGRPFFPEEIYERCK"
BASE COUNT         1088 a          690 c          614 g          919 t
ORIGIN      
        1 ttcctgtgaa actgtcttac cgtcaagaat aaccataaaa caattatatg gcagttagaa
       61 cctcatggcc agtaggggtg attaaaattg tatgttcaaa ttgggccgat aaagaaccgt
      121 cacgagtagt caccgtccag tggtcttttg aatttaaaac aatccgccag tctcgagaac
      181 aattaaccat cggttcaata gtgaaaatca tccctggctc taacaaaata tcttttcttt
      241 taatatcgaa atgataaaca aagggttctt cgtgaaactg aaggccaaca ccatgaccgc
      301 caagctcccg aacgacacta aaaccaaact tttttacata aggttcaata acttggccaa
      361 cacattcatt taaatattta cccggtttta aggctttaat tgatttatct aaagcatctt
      421 tcactacttt caataatttc tccgctttgg gactaatttg acctaccgga taagaagccg
      481 aaacatcacc ataataacca tctaaaatag cggtaatatc aatattaata atatcgccgt
      541 tttttaggat ggttttatca gaaggaatac catgacaaac cacatcgtta accgaagtgc
      601 aaatactttt agggtaaccg ttataatgaa gcggtgccgg cacccctcca tgctccacta
      661 aataatggtg ggcgttatca ttaagataaa gcgtagaaat tcctggtcta attaaagaac
      721 ccacccattt aagagtttct gccgccaatc ggcatgattg tctgattgcc ccaatttcct
      781 tttggttttt aattattacc acgtgatatt atacctctgt taaaataaac tcatgaaaac
      841 cagttggcaa caagtagctc cttggtataa aaaaattgta ggtaaaaaag gccattatta
      901 tcatcaacga gtaattatgc cccaagttgt tcgattaatg aatttaaaag ggggtgaagc
      961 cgtaattgat ttggcttgcg gcaacggggt tttaaaaaga aatttaaaga aaaaaataaa
     1021 atatgtcggt gtcgatctat ctaaaaattt aattgccgaa gctcaaagaa ttgaccataa
     1081 tagtcaatat ttagtagctg atgtcagccg agaattaaaa attaaaaaca aatttgactg
     1141 ggttgttatt attctagccc tgcaaaatat taaaaatttt cagggagtga ttagaaatgc
     1201 cagccagctt ttaaaaaccg gtggtaaatt actcatcgtt cttaatcacc cctgctttcg
     1261 cattccccgg caaagcagtt gggaaatcga taacagtaat aaaattgaat acaggcgcat
     1321 caatcgttat ctttcaccct tagctattcc gatcaaaatt aatggccatt ttacctggtc
     1381 tttccatcat tgtctcagcg attattttca ggctctaaaa gaaaatggct tcggtgttga
     1441 gacgctggag gaatggattt cagacaaaga tagtcaagga aatcgaaaaa aaatggaaaa
     1501 tcaagctaga aaagaatttc cactatttat ggcaatagtt gcttaccgaa ggctaaacaa
     1561 ttctgactaa tatctaataa ggcaaaaccg ggttgatcaa ataaatcgcc aatggctgtt
     1621 tttaaagccg cgggatcggt agctgaaacg gtttttaccc aaacatccgg attagccgct
     1681 tttattaatt taaccggatc aattggctta ttaggattac caagtggtgt cgatttagtt
     1741 ttagcttgaa acggagttgt tggtgaagtt tgaccagtag ttaaactata aagaaaatta
     1801 ttggccacca aaaccttgat cccaatatta gcccgagccg ccgccaaagt atgatttagt
     1861 ccctcaccat aaataccacc gtcaccgcca taaacagcga cggttaaatc cggcttaact
     1921 cttttaatcc cgctagccac cgccactgcc cgaccatgaa gagtatgaaa gccaaagcat
     1981 ttaaggaaat cggctaaatt accgctacaa ccaatatcat aacaaaaaac caattgataa
     2041 ggttgaatct ttttttcagc taaaacttgc tgaatttggg caaaaactaa aaaattaggg
     2101 cagccgggac accaggttgg cactattccg gttcgataat catttgcaac gttcataaat
     2161 ctcctccgga aagaacggcc gaccatcata cttagttatg attcggtcgg gcttaaaatt
     2221 aaattgggat tttaaaaggg tagttaattg gctactggca ttgttctcga ctacaactac
     2281 cgattgatag gaattaatta cagtttctaa ttgcggatca attggccaaa gggttttaat
     2341 cggcaaaata tcataatcct ccaaatcagc ttcccgaaca gctccagtta ccgaacccca
     2401 ggtaattatt agtttggtgg acccgcttac cgaccgaagg gtcgtagatg aaggtaaatc
     2461 aggtagaacg tttttgattt tagccaatct tttttccacc atttttttag tcacttcagc
     2521 gtcttcagta gaaaaaccct gatcatcatg ttcataacta ttcaccaaat attcctgacc
     2581 tttagtcccg ggcagaatcc gaggtaaagg agtcataata gtcttttctg ccgataaatc
     2641 ggtggtatca gctgccgatt ctcctaacat tttatcagaa agaaaaataa ccggcaattg
     2701 atgttttaca gccaaattaa ttgccaaacg ggataattca aaagattcct gctgagaacc
     2761 aggcgataaa acaattttag gaaattcacc atggccggca tggatagcga aaagcaaatc
     2821 cccttgactg gtccaagtgg gtaagccggt cgccggacca ggacgggaaa taagataaac
     2881 caccgcccca attccagcca tgcccaaaag gctgacagct tcagtcatta aagcaaaacc
     2941 accgcctgag gtaccagtag ccgctcgagc gccggcataa gccgctccgg cagctaaatt
     3001 aatcgcggcg atttcatctt cgggatgaac aacctgaaga tgataatctc cttggtgggc
     3061 cgctaaaaaa tgaagaatac ccgaagctgg cgtcatggga taagcagtat aaaaatcacc
     3121 tccggcttta agaaaacccc aaccagccgc ttcattacca tcgttataat tatgagataa
     3181 tttaaccgat ttatcaggca ttttaacttt aatttttgat tgattaaagt ttcgaaccgc
     3241 aaaatcaaaa ccaatttgaa aagctgtcca gttaatctcc gcatatttac cgaaatgggc
     3301 cttaataata t
//
LOCUS       DAOOIE010000055         3298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_22439_length_3298_cov_3.273204, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000055 DAOOIE010000000
VERSION     DAOOIE010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22439_length_3298_cov_3.273204"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="PK131_01965"
     CDS             <1..817
                     /locus_tag="PK131_01965"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005357387.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPD44920.1"
                     /translation="QLFEIMKGLPVKIRLLDPPLHEFLPKKEIDIKALAGEMGVSEEV
                     IRDKIETLQEFNPMMGHRGCRLGITFPEIFQMQVKAIIAAASEVNQNLTVEIMIPLIS
                     DVEELKWLKDKLIPVIEGTIKQGKKKIKYQVGVMIEVPRAALMADEIAQEADFFSFGT
                     NDLTQMTYGFSRDDANKFLKEYLKQKILKEDPFKQVDEKGVGQLMVMAVDKGRRVKKN
                     LNIGICGEQGGDPSTIKFCHKIGLDYVSCSPYRVPIAILAAAQAAVEEEKKSK"
     gene            complement(737..1600)
                     /locus_tag="PK131_01970"
     CDS             complement(737..1600)
                     /locus_tag="PK131_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44921.1"
                     /translation="MFDLTLSPANNEIFIKPGQNYLLAFQITNNSSTSLILSSSVESW
                     QPSGSQGEVNYHNTFMDPSISFSLNNADLKLGENFVLHPRENKQLVLKIMVSPLSNHQ
                     DYFYTFFVHQPADKFTGQLGGKIGAQLIISTQDKQTESLVIKKFSATPVIKDTFIKPI
                     NFHGYIYNNSSQFNKIAGKLTITKNDLLIEEFTLSPDTVLGGYSRLIRCADKNTEIIP
                     CALKPPLWPGFYQATLSLNLPQSPSATLNFFVFPYLILIILGILFGFFFFFYRRLGRR
                     QNSNRHSIGTT"
     gene            complement(1604..2341)
                     /locus_tag="PK131_01975"
     CDS             complement(1604..2341)
                     /locus_tag="PK131_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44922.1"
                     /translation="MKSKKYLLSFVFLGILGIWLLKFPKQSFSQRFQSNSYVIEWGNF
                     NMTGGTKTSTNYRLSDTVGQMAPGESNSNAYNLEAGFQYIYNTFNKFRFTINDPDLAI
                     NFGSLSPGVGITSSHSITISCPAGHGYQIMAEYNHPLQDLSSGITIPDTKCDLGSTCT
                     PTNANTWITNTSYGFGYNVAGVGATQYFPNITQFRPFTNTPVDIMTETTPVQNHTATV
                     TYKVLISSLQSAGNYENAITFIAVPNY"
     gene            complement(2331..2960)
                     /locus_tag="PK131_01980"
     CDS             complement(2331..2960)
                     /locus_tag="PK131_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44923.1"
                     /translation="MVLIIYFLITTLLAFVLSFYNFYIPQLIALAAIILILISIKTKV
                     VNIYLLSFIINLIIFGTGGLNSPVFFLIYFLLFTIAFQNRPSVTLTCSLISIVFLASS
                     LDLNSILALLSLLLITPLAWFVGHQFLEKQKSDVCLSRDETIFLFWLKLKFKTGISKI
                     IDSASILLSQPQLTPSQKEEVKFIRDSAKNLLNSSKKIADEIEHNADEI"
     gene            3116..3298
                     /locus_tag="PK131_01985"
                     /pseudo
     CDS             3116..3298
                     /locus_tag="PK131_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015562863.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
BASE COUNT         1174 a          430 c          746 g          948 t
ORIGIN      
        1 gcaattgttt gagattatga agggtttacc ggtgaaaatt agattacttg atccacctct
       61 ccatgaattc cttcctaaaa aggaaataga tatcaaagct ttagctggag aaatgggagt
      121 aagtgaagag gtaattagag ataaaattga gactttacaa gaatttaatc caatgatggg
      181 tcatcggggt tgtcgattgg ggataacttt tcccgaaatt tttcaaatgc aagtaaaagc
      241 gattatcgct gccgctagtg aggtgaatca aaatctaact gttgaaatta tgataccgtt
      301 gattagtgat gtagaagaat taaaatggct taaagataag ttaattccag taatcgaagg
      361 gactataaaa caaggaaaaa agaaaataaa ataccaagta ggggtgatga ttgaagtgcc
      421 gcgagcagct ttaatggctg atgaaattgc ccaagaagcc gattttttct cctttggcac
      481 aaatgattta acccaaatga cttatgggtt ttctcgggat gatgccaata aatttttaaa
      541 ggaatattta aaacagaaaa tcttaaaaga ggatccgttt aaacaagtag atgaaaaagg
      601 agtgggtcag ttaatggtaa tggcagttga taaggggaga agggtgaaga aaaatttaaa
      661 tattggtatt tgcggtgaac aaggcggcga cccaagtact ataaaatttt gccataaaat
      721 aggacttgat tatgtgtcat gtagtcccta tcgagtgccg attgctattt tggcggcggc
      781 ccaagcggcg gtagaagaag aaaaaaaatc caaataaaat acccagaata attaggatta
      841 aataaggaaa gacaaagaaa tttaaagtag cactaggaga ttgggggaga ttaagactaa
      901 gagttgcttg ataaaagccg ggccaaagag gaggtttaag ggcacagggg atgatctcgg
      961 tgtttttatc agcacagcgg ataaggcgtg aatagccgcc taaaacagta tcgggtgata
     1021 aagtaaattc ttcaattaaa agatcatttt tggtaatagt taattttcca gcgattttat
     1081 taaattgact cgagttatta tagatatatc catggaagtt aattggtttg ataaaggtgt
     1141 ctttaataac tggagtggca gagaattttt taatgaccaa cgattcggtt tgtttatctt
     1201 gggtggaaat tattaattgg gctccaattt taccgcctaa ttgcccggta aacttatcag
     1261 ccggctgatg aacaaaaaag gtataaaagt aatcctgatg gttacttagc gggctgacca
     1321 taatttttaa aactagctgt ttgttttccc ggggatgaag aacaaaattt tcacctagtt
     1381 ttaaatcagc attattaaga gaaaagctga tagaaggatc cataaaagtg ttatggtaat
     1441 taacttcacc ctgactgccg cttggttgcc aactttcgac ggaggaactt aagattaggc
     1501 ttgttgaaga attattggtt atttggaaag caagtagata attttgcccg ggtttaataa
     1561 aaatttcatt gttagctggt gagagagtta aatcaaacat attttaataa tttgggacag
     1621 caataaaagt aatggcattt tcgtagttac cggctgattg aaggctagaa attagaacct
     1681 tgtaagtaac agtagcggta tggttttgaa caggagttgt ttctgtcatg atatcaactg
     1741 gagtgttagt gaaaggtctg aattgagtga tgttagggaa atattgagtg gcaccaacgc
     1801 cagcgacatt ataaccaaat ccataactag tgttagtaat ccaagtatta gcattggttg
     1861 gagtgcaggt ggaaccaagg tcacatttgg tatcaggaat ggtaataccc gatgaaagat
     1921 cttgtagtgg atgattatat tcagccataa tttgataacc atgacctgca gggcaggaaa
     1981 tagtgatcga atgagaggaa gtaataccga ctcccggtga taaactacca aagttgatag
     2041 ctaaatctgg atcattaata gtaaaacgga atttattgaa agtgttatag atgtattgga
     2101 aaccggcctc gagattatag gcattactat tagattcgcc aggagccatt tgaccaactg
     2161 tatctgataa acggtaatta gttgaagttt tagtccctcc agtcatatta aagttacccc
     2221 attcaatgac gtaactgtta gattgaaatc gttggctaaa actttgtttg ggaaatttca
     2281 ataaccaaat tcccaaaatc cccaaaaaca caaaagagag taaatatttt ttagatttca
     2341 tcagcgttat gttcgatttc atcagctatc tttttagaag aattgagaag gtttttggct
     2401 gaatcacgga taaatttgac ttcttctttt tggctgggag taagttgggg ttgggagaga
     2461 aggattgagg ctgaatcaat aattttactg atgccggttt taaacttaag tttcagccag
     2521 aaaagaaaga ttgtctcgtc gcgtgaaaga caaacatccg atttttgttt ttcaaggaat
     2581 tgatggccga caaaccaagc taagggggtg attaggagta aagaaagtaa agctaggatc
     2641 gagtttaagt caagactgga agctaaaaaa acaatagaaa ttagagagca agttaaagtt
     2701 acactaggtc gattttgaaa agcaatggta aaaaggagaa agtaaataag aaaaaaaaca
     2761 ggtgaattta aaccaccggt tccaaagata atcagattga taatgaaaga gagtagataa
     2821 atattaacga ctttggtttt gatagaaata agaattaaaa taatggcggc taaggcaatt
     2881 agttggggaa tatagaaatt ataaaaggat aaaacaaagg ccaacaaggt ggtgattaga
     2941 aaatagataa taagaaccat agaaatagta tatattaaat ttagtggaca aaaagaaaat
     3001 agttaattgg ctagtgattt taggaatttt gtttattact tttggcattg gaattaattt
     3061 aaggcagcag gcaaaacccc aggtggaaat tataaaaaag gcagttaaga ttgataattt
     3121 agttaatatt aatacggctg atttgggtga attggatact ttgccaggaa ttggaccaaa
     3181 aacggcccag aaaattattg actatcggat taatcacggg ggtttcaaaa gtattgacga
     3241 gataaaaaat gttagtggaa ttggaccgaa aaagtatgca gacttggtta ctaaaatt
//
LOCUS       DAOOIE010000056         3243 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_22913_length_3243_cov_4.071205, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000056 DAOOIE010000000
VERSION     DAOOIE010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22913_length_3243_cov_4.071205"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="PK131_01990"
     CDS             complement(<1..1039)
                     /locus_tag="PK131_01990"
                     /inference="COORDINATES: protein motif:HMM:NF015672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44924.1"
                     /translation="MKLIYLNLRFPIGELEKIRDRFIVYLNSGKLRKTTLYYPKEERH
                     HCQVIAIGGSNCDSAKVVWHHGKIELKEIKNDKIPKFTDKNIFLSYLADKIKNDTDLL
                     ILNLAQEIEPIIRENRIDGILKQASKEHRFDGLVGEIIGQTIENYLGQKGRKIEVVVV
                     NDVVGLALNKAKNENWRHFLAGVLGTGMNLGLYENEKVIINSEAGDFNSLQLTETGKL
                     IDRESASPGRHLIEKEVAGGYLFQHFNLICQKMAIEGRVENTEAMGKMAQEAGWRGEI
                     ARQLFERSAQLWAAQIAGAARQKGEGKIKVLMEGSLFWKGYNYQNYVKKYLAILGINP
                     HEIDFFLIENSN"
     gene            1482..2951
                     /locus_tag="PK131_01995"
     CDS             1482..2951
                     /locus_tag="PK131_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44925.1"
                     /translation="MVEREGKLEVGPRPENVGQLGRYEITAWADAEERAIGIREKVLT
                     DWVKYSGFDRKTLEYLFDTQAWVTNPISPFYSQKRRELDTGKKEFVALSAWDFNGCLS
                     LHRDGYDPQHTFCPELEAAIKMQIERLGSFFIISALTSEQMAYHLAKEKNTAGNIIMA
                     SENGRGWLIPSKEGSSIKLMEFKLPLSEKNEKALIHLKEIVNDLTAVMRQEGLVSFVN
                     DQKFSKCTMEGAANGREWYRATIEKLLLPYLAAKGAVWNGNPERFSLDGIGFAVSPTP
                     ETWEIEVIEGTDKIGKRLAREILHNTLTDITLSMKEYTYFRLLTGGDSVKWGGSDVGL
                     TDPNAATIPFAVPAAEKDPRLSLDQLKEFIQYQRKYWPTYVRRGEEVKLAGAGIAPAD
                     FMKTVIETVPCLFLPTQDFAQFLAARLTEVYGYLPGNEFDLSQPLLLYNFPADVSDEQ
                     KELFLKAGKDLPIPADDKEAVARFISLLRQRAGLAAEEI"
     gene            complement(2913..>3243)
                     /locus_tag="PK131_02000"
     CDS             complement(2913..>3243)
                     /locus_tag="PK131_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44926.1"
                     /translation="PGECGRYIDVNQDNFCDHSQISKNSFGFNYLIAIVTVLVILYIF
                     SVKYLKNYKKIWNWVLSISFTLTAITGWFWHFLHLPAGVITLVISLFHCLWHWSYFFS
                     RQPRPLS"
BASE COUNT          850 a          649 c          762 g          982 t
ORIGIN      
        1 gattgctgtt ttcgattaga aaaaagtcga tttcgtgggg atttataccc aaaattgcta
       61 gatatttttt gacgtaattt tggtaattgt atcccttcca aaataggctt ccttccatca
      121 gcacttttat ttttccttct cctttttgtc gagcggcgcc ggcgatttga gccgcccaga
      181 gctgggccga tctttcgaat aattgtctgg cgatttctcc ccgccagccg gcctcttggg
      241 ccattttacc catagcttcc gtattttcaa ctctgccttc aattgccatt ttttggcaaa
      301 ttaggttgaa gtgctggaaa agatatcccc cggccacttc tttttcgatt aagtgccggc
      361 cggggctggc cgattcgcgg tcgatcagtt taccggtctc cgtcagctgg agagagttga
      421 aatctcccgc ctccgaatta atgattacct tttcattttc gtaaaggccc agattcatac
      481 ctgttcctaa gaccccggcc agaaagtggc gccagttttc gtttttcgcc ttatttaacg
      541 ccaatccaac tacatcattg actactacca cttcgatttt tctgcctttt tggccgaggt
      601 aattttcgat agtttggccg attatttccc cgaccaagcc gtcaaaccga tgctctttcg
      661 acgcttgttt taaaatgccg tcgattctat tttcccggat aatcggttcg atttcctggg
      721 cgagattcag aatcagtagg tcggtatcat ttttaatttt gtcggctaga taggacagga
      781 atatgttttt gtccgtaaat ttgggaattt tgtcgttttt aatttcttta agttcaattt
      841 tgccatggtg ccagactact ttggctgagt cacaattact gccgccgatg gcgattactt
      901 ggcagtggtg tcgttcttcc ttggggtaat atagggtagt ttttcttaac ttgcccgaat
      961 taagataaac tataaaccga tcgcggattt tctctaattc cccaatcggg aaccgaagat
     1021 ttaaataaat tagcttcatt ggcggtttat tatagcatta cttatagtat tgacatatag
     1081 tgtatggtgt gttacactaa ttttgttttg acagacttaa gacttaacag tctttagcga
     1141 attgctcatt tttcgagatt cccctcggcc attaaaaact ggatggattt tgatttttgt
     1201 ttggtgttgt ttaggagaca aagttaagca ggttttaccc cctatcgtac tcacagtgtt
     1261 gtggtttctt ttttctaaca ccaccaaata gaaatcaaat ttttaaatca gacttaacag
     1321 tctttagcga attgctcatt tttcgagatt tcccctcggt ttttagtaat tagaaaaaaa
     1381 atgaaaaagg attatgttgc ttctcaaaaa atagaaaaat tggcgggaca aggtaccttg
     1441 ggctttgggg aactgttaaa gattttgaag gaggaacggt gatggttgaa agagaaggta
     1501 aactagaagt tggaccccgg ccggaaaatg tcggccaatt aggcaggtat gaaattaccg
     1561 cctgggccga tgccgaagag agagctatcg gtattcggga gaaggttttg acggactggg
     1621 tgaaatacag cggtttcgac cggaagactt tagagtattt attcgatacc caagcttggg
     1681 taactaatcc catcagccct ttttactccc agaagcgaag ggaattagat accggtaaga
     1741 aggagtttgt agccctgtca gcctgggatt ttaatggctg tctttccctt catcgggacg
     1801 gctacgatcc gcaacacact ttctgcccgg agctggaggc agcgattaag atgcagattg
     1861 aaaggttggg ttcatttttt attatttcag ccttaacatc ggaacaaatg gcttatcatc
     1921 tggctaagga gaaaaataca gccggcaata ttattatggc ttcggaaaac ggccggggct
     1981 ggctgatccc ctcgaaagaa ggcagttcaa tcaaattaat ggagtttaaa ttgcctttgt
     2041 cggaaaagaa tgagaaagct ttgattcatc tcaaggagat agttaatgat ttaacggcgg
     2101 tgatgagaca ggagggattg gtgtcttttg tcaatgatca aaaattttcc aagtgtacca
     2161 tggagggagc ggccaacggc cgggagtggt atcgagcgac gattgagaaa ctacttttgc
     2221 cttatcttgc agctaaaggg gcagtttgga acggtaatcc ggagagattt tccctggacg
     2281 gcatcggttt tgccgtttct cctactcccg aaacctggga aatagaagtg attgaaggga
     2341 ctgataaaat tggcaagcgt ttagctagag aaattttaca taatactcta accgacatta
     2401 ccctatctat gaaagaatat acctatttta ggctgttgac gggaggcgat tcggtcaaat
     2461 ggggcggaag tgatgttggt ttgaccgatc ctaatgccgc tactattccc tttgctgtcc
     2521 ccgcggcgga gaaggatccc cgtttgagcc tggatcaatt gaaggagttt attcagtatc
     2581 agcggaaata ctggccgacc tatgtcagaa gaggagagga ggttaaactg gccggggcgg
     2641 gcattgctcc ggcggatttt atgaaaacgg tgatcgaaac agtcccttgt ttgtttcttc
     2701 ctacccaaga ttttgcccag ttcttggcgg ccagactaac cgaggtttat ggttatcttc
     2761 ctgggaatga gtttgattta tctcagcctc tgcttctata taatttcccg gctgacgttt
     2821 cggatgaaca aaaagagctg tttttgaagg cagggaaaga tttaccaatt ccggctgatg
     2881 acaaggaagc agtagcccga tttatttctc ttttaagaca gcgggcgggg ctggcggctg
     2941 aagaaatatg accaatgcca caggcagtga aagaggctaa ttactaaagt aatgaccccg
     3001 gccggtaaat gtaaaaagtg ccagaaccag ccggtaatag cagtcaaggt gaagctgatt
     3061 gacagaaccc aattccaaat ttttttataa tttttcagat atttaactga aaaaatataa
     3121 agaatgacca ggacagtaac aatggcgatc aaatagttga aaccgaagct atttttgctt
     3181 atttggctgt ggtcacagaa attgtcttga ttaacgtcaa tataccggcc gcattctccc
     3241 ggg
//
LOCUS       DAOOIE010000057         3228 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_23052_length_3228_cov_3.805232, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000057 DAOOIE010000000
VERSION     DAOOIE010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23052_length_3228_cov_3.805232"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="PK131_02005"
     CDS             complement(<1..877)
                     /locus_tag="PK131_02005"
                     /inference="COORDINATES: protein motif:HMM:NF025755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-transfer associated ATP-grasp
                     domain-containing protein"
                     /protein_id="HPD44927.1"
                     /translation="MAKFRKLRQFLTKNERNKVFLRKNKPSARIVADSKLKTKKILAK
                     SGINVPCLITKFSRQGDLDQFDWRKLEGNFVIKPASGYAGEGIIIIRKRGKYADEWVG
                     MDGSVITTGDLIRHCREILGGKYSLYNMPDEVIVEERIKIHPMFLSITKSGTPDIRVI
                     VYNQVPVMAMLRIPTEKSGSRSNLQQGAIGLGIDLATGITTFGIEGKGDIIRKIYDWG
                     KKKMRKVNGIKIPLWREILETAVKCQKAVPGLGFLGVDVVLDKEKGPCVLELNARPGL
                     SIQICNRAGLKARLDK"
     gene            complement(919..1506)
                     /locus_tag="PK131_02010"
     CDS             complement(919..1506)
                     /locus_tag="PK131_02010"
                     /inference="COORDINATES: protein motif:HMM:NF025760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM domain-containing protein"
                     /protein_id="HPD44928.1"
                     /translation="MNPMLILLPVVATLVSFLHYFIGLTGYGIFMPTMMAVTFLSMGV
                     LEGLVLFGLILVISLVSNAILKKFRLHFWPARAINLAFIGIGVSSLSDWVYRQYSLNL
                     SNIYGVMIMILLVEEFVHTQLVKSKGEAKRLILGTLVLAIIGAEVMSIAGIQNWVLAH
                     PYLIILLIVIANIIIGNYRGMRLIEIGRFGKAIRR"
     gene            1555..1881
                     /locus_tag="PK131_02015"
     CDS             1555..1881
                     /locus_tag="PK131_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44929.1"
                     /translation="MNFGIGVSIDLPGSNSQTVSQNFPSFGFLISRTIAIAGVVAGII
                     LVGMAIYTGFLFMSSAGTGDAKRISRAQNSLTTSLIGFLLIFITYFIIQIIQNITGVP
                     ILNGTL"
     gene            1868..2221
                     /locus_tag="PK131_02020"
     CDS             1868..2221
                     /locus_tag="PK131_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44930.1"
                     /translation="MALFKPIVLAAEGLGKVTPPPWLGKWIKPGDEPGQGLFYFLSVL
                     LSLAVTIAGIYLVFQFIFAGYMYLSAGGDPKKVELAGAKLWQSLLGLVIVAASLTLAG
                     VITRLTGISIFKFTF"
     gene            2226..2594
                     /locus_tag="PK131_02025"
     CDS             2226..2594
                     /locus_tag="PK131_02025"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44931.1"
                     /translation="MINLKLIKPILAYCLTDKDGNCLPNNPTAMTTQLEKYLSTIIGF
                     ITLVGVIWFTFQIIFAGYTFLSSGGDIKKVERAQQQLMQSLIGIIVIIAATIIVGLIV
                     RILGLGNIFDLNLFFRNLNL"
     gene            2596..2919
                     /locus_tag="PK131_02030"
     CDS             2596..2919
                     /locus_tag="PK131_02030"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44932.1"
                     /translation="MAICNPLVDKNCSDIVSTAPDRASKLITGIISLIFGAAIIYFIF
                     TLLMAGFSFINSGSDEKQLAAAKSQLTNAFIGLFVLLCVYALVALLEKIFGIQLLKVT
                     LPTLT"
     gene            2962..>3228
                     /locus_tag="PK131_02035"
     CDS             2962..>3228
                     /locus_tag="PK131_02035"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HPD44933.1"
                     /translation="MLNLIQPIWAQETFDLTPKNEFAPLGKFRVNNLVQTLFSVLLVA
                     VGLVFLFMLILGGLKWMTSEGDEKKLAAARGQITNALIGLVIVFS"
BASE COUNT          920 a          691 c          526 g         1091 t
ORIGIN      
        1 ctttatccag cctagccttt aaaccggctc gattgcaaat ctggattgac aaaccgggtc
       61 gagcatttaa ttccaaaaca cacggccctt tttctttatc taacaccaca tccaccccta
      121 aaaaacctaa tccgggtact gctttttggc atttaaccgc tgtttctaaa atttcccgcc
      181 atagaggaat tttaattcca ttaaccttgc gcattttttt cttaccccaa tcatagattt
      241 tcctaataat atcccccttg ccttcaatac cgaaggtggt aattccggtg gctaaatcaa
      301 ttcctaaacc aattgcaccc tgttgtaaat ttgacctact accacttttt tctgttggaa
      361 ttctcaacat agccataact ggtacttgat tatagacaat tacccgaata tcaggtgtac
      421 ctgatttagt aatagacaaa aacataggat gaattttaat tcgctcttct acaattacct
      481 cgtcgggcat gttatataaa ctatactttc ctcctaaaat ttcccggcaa tgtctaatta
      541 aatccccggt agtaattacc gacccatcca tcccaaccca ttcatcagca tatttgcccc
      601 tctttctaat aataatgatt ccctctccgg catagcccga ggctggctta ataacaaaat
      661 taccctccaa tttcctccaa tcaaactggt ccaaatcccc ttgtcggcta aatttagtta
      721 ttaagcacgg aacattaatt cccgatttag ccaagatttt tttggttttt aatttactgt
      781 cggctactat tctggcggat ggtttatttt tacgtagaaa aaccttgttc ctttcgtttt
      841 tggttaaaaa ttgccgtaat tttctaaatt ttgccattgg taatttgtaa cctgcccagg
      901 caggttttta atttttaatt atctcctaat ggcctttcca aatctaccta tctcaatcaa
      961 tctcatccct cggtaattgc caataataat atttgctatg acaattagta aaataataag
     1021 atagggatga gctaaaaccc agttttgaat tccggcaata ctcattactt ccgccccaat
     1081 aatagccaaa accaaagtgc ctaaaattaa ccttttagct tcacctttac ttttaactaa
     1141 ctgggtatga acaaattctt ccaccaggag aatcataatc ataactccgt aaatattgct
     1201 taaattaaga ctatattgtc ggtaaaccca atcggataaa ctactaaccc caatcccaat
     1261 aaaagccaaa ttaattgctc tggctggcca aaagtgaagc ctgaatttct tgagaatagc
     1321 gttactgact aaggaaatta ctaaaatcaa tccgaaaaga accaagcctt ccaaaacccc
     1381 cattgataaa aaggtgacag ccatcattgt cggcataaaa ataccataac cggttaagcc
     1441 aataaaatag tggagaaatg aaactaaagt cgctactacc ggcagtaaaa tcaacattgg
     1501 attcataaat tttgtgccgt attataccac aatccaatct gttataattt ttttatgaat
     1561 tttggaattg gagtaagcat tgacctgcct ggtagcaata gccagactgt tagtcaaaat
     1621 ttccccagtt ttggttttct tatcagccgg actattgcta ttgctggagt agtagccggt
     1681 attattcttg tgggtatggc catttatacc ggttttctct ttatgagttc agctggtacc
     1741 ggcgatgcta aaaggatcag ccgggcccaa aacagtctaa ctacctcttt aattggtttt
     1801 ttgctaattt ttatcaccta ttttataatt caaataattc aaaatattac tggtgttcct
     1861 attcttaatg gcactcttta aacctatagt tttggcggct gagggattgg ggaaagttac
     1921 gcccccgcct tggttgggca aatggattaa acccggggat gagcccggcc aaggtctctt
     1981 ttacttttta agtgttttgc ttagcttagc agttactatc gcgggcattt atttggtttt
     2041 ccaatttatt ttcgctggtt acatgtatct ttcggccggt ggcgatccta agaaagtcga
     2101 attagccgga gccaaacttt ggcaaagcct gctgggttta gtaattgttg ccgcctcttt
     2161 aaccttggcc ggagttatta ctagacttac cggcatttct atttttaaat ttactttcta
     2221 atctaatgat taacctgaaa ttaattaaac caattttagc ctactgtctt actgataaag
     2281 atggcaactg tctacccaat aatccaacag caatgactac ccaactagaa aaatatttat
     2341 ctaccattat tggctttatc actttagtcg gcgttatttg gtttacattc caaataattt
     2401 ttgccggcta taccttcctc tcctctggtg gtgatattaa aaaagtagaa agggctcaac
     2461 aacaattaat gcagtcttta atcggtatta ttgttatcat cgccgctact attattgttg
     2521 gtttaatagt tcgaattttg ggtttaggta atatttttga ccttaatctt ttctttagaa
     2581 atttaaatct ctaaaatggc tatttgtaat cctttagtag ataaaaattg cagtgatatt
     2641 gtttccacag ctcccgatcg agcttccaag ttaattaccg gtatcatcag ccttattttt
     2701 ggagccgcga tcatttactt tatttttacc ttacttatgg caggattttc atttattaac
     2761 agcggcagtg acgaaaagca gttagccgca gccaaaagtc aattaaccaa tgcttttatt
     2821 ggcttatttg ttcttctttg tgtttatgct ctagtggctc tcttggaaaa aatctttggg
     2881 attcaactgc tcaaagttac ccttcccact ttgacctaaa aactgctaca attagttacc
     2941 attattaatt aattaataaa catgctcaat ctaatccaac caatttgggc tcaggaaact
     3001 tttgatctta cccccaaaaa cgagtttgct cctctgggta agttccgggt taataatctg
     3061 gttcaaaccc ttttttcagt tcttttggtg gcagtaggtc tagttttcct cttcatgctc
     3121 attctcggtg gtcttaagtg gatgacatct gaaggtgatg aaaagaagct ggcagcagct
     3181 agaggtcaga ttaccaatgc ccttatcggc ttggtaattg tcttctcg
//
LOCUS       DAOOIE010000058         3190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_23402_length_3190_cov_3.464434, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000058 DAOOIE010000000
VERSION     DAOOIE010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23402_length_3190_cov_3.464434"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="PK131_02040"
     CDS             complement(<1..771)
                     /locus_tag="PK131_02040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008634270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HPD44934.1"
                     /translation="MAKTTLADLGLEQKLWAAADKLRGHMDPGEYKHVVLGLIFLKYI
                     SDSFEETYQSIKNDPDAFEGEWEDKDAYNARNIFWVPEIARWEYLKQNAKKPEIGKIV
                     DDAMIAIEKENPSLKGVLPKRYARPDLNKQRLGEIIDIFSSLGLGDQEAKSKDILGRV
                     YEYFIGRFASAEGKGGGEFYTPASVVKLLVSMIEPYKGRVYDPCCGSGGMFVQSERFI
                     QEHGGSIGDISVYGQESNLTTWKLAKMNLAIRGIENNLG"
     gene            complement(1047..1117)
                     /locus_tag="PK131_02045"
     tRNA            complement(1047..1117)
                     /locus_tag="PK131_02045"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1083..1085),aa:Gln,seq:ctg)
     gene            complement(1159..1884)
                     /locus_tag="PK131_02050"
     CDS             complement(1159..1884)
                     /locus_tag="PK131_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HPD44935.1"
                     /translation="MTENQKKLADLLIATKTEAKVRRREQLVDGSYRFYEVVRPTSPI
                     DFAREGEFALKLHEKNPTAPLSPIYINLRNLPEDLLKHMGLVLAEITKGSKADFCAGI
                     PDAGVPIARAYAAAAGMEIKDVLAKAETKEGRRIVKTDLGHEKELTGKRVRLIDDLVT
                     QADTKLEAIRAAEEAGLKVADIVVLVDREQGGAKQLAEAGYQIRAAFKLSQLLDYYLM
                     TGQINRTKYDRVNYYLTNSASKA"
     gene            complement(1887..2864)
                     /gene="pyrF"
                     /locus_tag="PK131_02055"
     CDS             complement(1887..2864)
                     /gene="pyrF"
                     /locus_tag="PK131_02055"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020586810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPD44936.1"
                     /translation="MNNKIELGSFPEASELSKCKFMVKLEERWSRHNTLVCVGLDSDE
                     EKIPRGESIERFNQQIVDKTHDLVCAYKPNTAFYEARGFAGWLTLEETINYIKENYPE
                     IPVILDSKRADIGNTNLGYTRMAFDRMGADAITVNPYLGSTFFDGKEIKLEALQPFLE
                     WKNKGIIVLAKTSNKSGGEIQDLPIDLRQLTTYSDKFGRMDELREKIGSDIVPLWSIV
                     AYRVAKYWNVNGNCGLVVGATYPEELGLARKIVGDKIPILVPGAGKKRGQGGDVEAAV
                     KAGIDSRKQGIIINSSREIIFASSDPTDFAQAARKAAQELKEEINQFRQ"
     gene            2969..>3190
                     /locus_tag="PK131_02060"
     CDS             2969..>3190
                     /locus_tag="PK131_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44937.1"
                     /translation="MKIKILPLLLVVIGLVLRLSPHPVNFAPISAIALFSGVYLPKKW
                     NLVLPLLVLLLTDYFLGFYGALMIFTYGAY"
BASE COUNT          884 a          750 c          534 g         1022 t
ORIGIN      
        1 tcctaaattg ttttcaatac cccgaatggc caaattcatc ttggccaatt tccacgtcgt
       61 caggttgctt tcctgcccgt agactgaaat atcgccaata cttcccccat gttcctgtat
      121 aaatcgttca ctttgcacaa acataccgcc actaccgcaa cacggatcgt aaactcggcc
      181 tttgtatggc tcgatcatgg aaacgagaag ctttaccact gaagcgggcg tgtagaattc
      241 accgccacct ttaccctcgg cactggcaaa tctgccgata aaatactcat acactcggcc
      301 taatatatcc ttagatttag cttcctgatc tcccaaccct aaagagctaa aaatgtcgat
      361 gatttcaccg agccgttgtt tattgagatc ggggcgggca tagcgttttg gcaatactcc
      421 ttttaatgat gggttttctt tttcaatggc gatcatagca tcatccacaa tcttgccgat
      481 ttctggcttc ttggcgtttt gctttagata ctcccaacgg gcaatttctg gtacccaaaa
      541 tatattacgg gcattgtagg cgtccttatc ctcccattcg ccctcaaatg catcgggatc
      601 gtttttaatt gattggtaag tttcttcaaa agagtcggag atatatttta gaaagatgag
      661 gccaagtacg acatgcttat attcgccagg atccatgtgg ccccgtaatt tatctgccgc
      721 tgcccacaat ttctgttcaa ggcccagatc agctaatgtg gtttttgcca ttatgaaccc
      781 attttatcac tttattcgta cttatattta tgactaaaac tctaactaac tcacactaac
      841 tcttaataaa gtgccgtatt ttcgggataa atatttaaca aatatttatt aagtgttatt
      901 atattagtaa ttcttcaaaa agaatttgag gcgtacttat taaaggaccc acaaagaaag
      961 aagtcctttt ttaatgagta acaatttaaa aatcgagtac atatcagtta aaaacctaaa
     1021 tctcggtctc ttggtgtcca atcatgttgt ccggcaagga ttcgaacctc aattagttgg
     1081 ttcagagcca actgtcctac cattagacga ccggacaata ccgactatat tttatcctat
     1141 cttcaaaaac ttctctacct acgccttact agctgaatta gtcaagtaat aattaacccg
     1201 gtcatatttg gtccgattga tttgacccgt cattaggtaa taatcgagaa gctgagataa
     1261 tttaaaagca gctcgaattt ggtagccagc ttcagctaat tgcttagccc cgccctgttc
     1321 acgatcaact aaaaccacaa tatcggctac ttttaaacca gcctcctctg ccgccctaat
     1381 tgcctctaat ttggtatctg cttgagtaac taaatcatca attaacctga ctcttttacc
     1441 tgtgagttcc ttttcatgac ctaaatcagt cttaacaatt cgccgccctt cttttgtctc
     1501 agctttagcc agaacgtctt taatctccat gccggcagcg gccgcataag cccgggcaat
     1561 cggtaccccg gcatcaggaa taccggcaca aaaatccgct ttactaccct tagttatttc
     1621 cgccaatact aaacccatat gtttaaggag atcttccggt aaattacgca aattaatata
     1681 aatgggtgac aagggggcgg tcggattttt ctcatggagt ttaagggcaa attccccttc
     1741 ccgggcgaaa tcaatcggac tcgtcggccg taccacttca taaaaacgat agctgccatc
     1801 gactaattgc tcccttcttc tcacctttgc ttccgtttta gttgcaatta gtaaatcagc
     1861 caattttttt tgattttcag tcatatttat tgcctaaatt gattaatttc ctcttttaac
     1921 tcttgggcag cttttctagc agcttgggca aaatctgttg gatcagatga agcaaaaatt
     1981 atctctcggg aagaattaat aattattccc tgcttccggc tatctatccc agccttaact
     2041 gccgcttcaa catccccgcc ttggcctctc tttttacctg ctccaggcac aagaataggt
     2101 attttatccc caactatttt tctcgccaaa cctagttctt ctggataggt cgccccgacc
     2161 accaaaccac aattgccgtt tacattccaa tatttagcca cccgataagc cacaattgac
     2221 caaaggggaa caatatcact cccaatcttt tcccgtaatt catccattct gccaaattta
     2281 tcgctatagg tcgttaattg tctcaaatca atcggtaaat cttgaatttc gccccccgat
     2341 ttattggaag ttttagccaa aacgataatc cctttgtttt tccattccaa aaatggctga
     2401 agagcctcta gtttgatctc tttcccatca aaaaaggtcg atcccaaata gggattgact
     2461 gtaattgcat cggcccccat tcggtcaaaa gccattcggg tataaccaag attagtattg
     2521 ccgatatcag cccgtttgga atcaagaata accggaattt cgggataatt ttctttaata
     2581 taatttattg tctcttcaag tgtcagccag ccggcaaaac ctctggcctc ataaaaagca
     2641 gtattaggct tataggcaca aactaaatca tgggtcttat cgactatctg ctgattaaat
     2701 ctttcgatcg attctccccg gggaattttt tcctcatcgg aatcaaggcc aacacaaact
     2761 aaagtattat gccgcgacca acgttcctct aatttaacca taaatttaca tttagataat
     2821 tccgatgctt cgggaaaact gcccaattct attttgttat tcattctttt ttgatcgata
     2881 aataaatcga taaataaact aaaaggtagg ggttaaaaga tattatagga attttaaaat
     2941 taaaaacaag ggttataata tctaaaagat gaaaataaaa attttgcctt tattattagt
     3001 tgttataggg cttgtccttc gcttgtcgcc tcatccggtt aattttgccc ccattagtgc
     3061 tattgccctt ttttccggtg tttatctgcc taaaaaatgg aatttagttt tacccttgtt
     3121 ggtcttatta ttaactgatt atttcctcgg cttctatggt gctctcatga ttttcactta
     3181 tggtgcctat
//
LOCUS       DAOOIE010000059         3187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_23439_length_3187_cov_2.588761, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000059 DAOOIE010000000
VERSION     DAOOIE010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23439_length_3187_cov_2.588761"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="PK131_02065"
     CDS             <1..299
                     /locus_tag="PK131_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44938.1"
                     /translation="PTGSKLIKMTGSEVEPVQYEELGKQVFEGFYGDKFPLYAQSSIK
                     TSIQYQSSIPPSTDYRLLLQKQPGTKAIDYEIYVNGKKYDSFSWVADKTIKIAF"
     gene            299..859
                     /gene="recO"
                     /locus_tag="PK131_02070"
     CDS             299..859
                     /gene="recO"
                     /locus_tag="PK131_02070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPD44939.1"
                     /translation="MSKYKTVQGIVINKKPTRDNDLIITLLTPNEGKIVCLAKGARSI
                     KSHRLGHLQLGNILKVSIYQKGDFRWLTESQTLQAFLQTPKKLVQLNLLFLFLEFINQ
                     LVPENQINPGLYQICQKIIKAIDKNQFKSYINYEINLIQLLGFGTPPEIINAYQANEL
                     KTAQNLIKNFLESIIEKPVKSNQLFR"
     gene            965..2203
                     /locus_tag="PK131_02075"
     CDS             965..2203
                     /locus_tag="PK131_02075"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPD44940.1"
                     /translation="MDQTRNETVAKVVALCKRRGIVFPNSEIYGGIQGFYDYGPLGTA
                     MKQNWKNIWWQENVVRRHDVQGIDGAIITNPQVWQASGHIKNFTDIEDGKKFNIMFET
                     EVGIKKGDKIHTYLRGEACQDIYLNFKNVVDSTRVKIPFGIAQIGKAFRNEITPKNFL
                     YRTREFEQWDIEFFIHPKDEKFWFNYWQKNRLEFYRQYFSQTSKLRLRRHEKNELVFY
                     AKEAFDIEYQAPWGWAEMEGIHNRGDYDLKQHSQFSHQDLQYLDQQSHEKYFPYIIES
                     SGGVDRSFFFLILDAYQEEKLKNGETRIVLKIHPKIAAYKIAVIPLAANKPELIDLAE
                     KIYSRLSLKYAVDFDDSSNIGKKYRRQDEIGTPWCLTVDFQTLQDKTVTLRDRDNLNQ
                     IRLKIEEVDNWLERQLNKPR"
     gene            2200..2727
                     /locus_tag="PK131_02080"
     CDS             2200..2727
                     /locus_tag="PK131_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44941.1"
                     /translation="MKKLFLVLISAFLLFGCGLKKTTPTVTPTPASTTFELTDKEMPE
                     INLTASDDGHWLTLKINKIPSSIVNIEYELLYRASESGLEIEKGLGDTLKVEGRSLER
                     KLLLGTESCTNGCKYKFDENVTGGDLNLTLINQQGQTATIQKFFTLVKDKKSKKIVAT
                     LTDASSSASPAQGNN"
     gene            2687..>3187
                     /gene="mraW"
                     /locus_tag="PK131_02085"
     CDS             2687..>3187
                     /gene="mraW"
                     /locus_tag="PK131_02085"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HPD44942.1"
                     /translation="MPLHQPVLLKEITRLFQPGPGKIFIDATLGNGGHSLKLLQAGAK
                     VYAFEFDPENIKVVQKRIHHPNLTIINDNFSHINHYFNFKVNGILFDLGLSINQQKAQ
                     GRGFSFNDALSLDMRLNPQQQTLTAQKIVNTYSQKELFDLFSKYAQEKNSHLISQLII
                     KNRPFNN"
BASE COUNT         1092 a          593 c          540 g          962 t
ORIGIN      
        1 ttcctactgg ctcgaaacta attaaaatga caggatccga agtagaacca gtccaatatg
       61 aagaattggg caaacaagtt tttgagggtt tctacggcga taaatttccc ctatatgccc
      121 aatcttcaat aaagacgtct attcaatatc aaagcagtat tcctccttcc accgactatc
      181 gtctcttact tcaaaaacaa ccaggaacca aggctatcga ttacgaaatt tatgttaacg
      241 gaaagaaata tgattctttt agctgggtcg ccgataaaac cattaaaatt gctttttaat
      301 gtcaaaatat aaaaccgttc aagggattgt tatcaataaa aaaccaacta gagataatga
      361 tctgattatt accctgttaa cccccaatga gggtaaaatt gtctgtttag ctaaaggtgc
      421 tagatcaatt aaaagccatc ggttgggtca tttacaacta ggtaatattc ttaaagtttc
      481 aatctatcaa aaaggtgatt ttagatggct gactgaatct caaacccttc aagcttttct
      541 ccaaacccct aaaaaattag ttcaattaaa cttacttttt ctctttttag aatttattaa
      601 tcaattagtt cctgaaaatc aaattaatcc tggtctttat cagatctgcc aaaaaataat
      661 taaggctatt gataaaaatc aatttaaatc ctacatcaat tacgaaatca atctgattca
      721 attattgggt tttggtactc caccggaaat tatcaatgcc tatcaagcca acgaactcaa
      781 aacggctcaa aatttaatta aaaattttct tgaatctatt attgaaaaac cagttaaatc
      841 aaaccaatta ttccgataaa actaaaggtg ggctcttgac aatttactca aaatatgtta
      901 tcctataagg tgataggtga aaagctccga tttattatcc cgtcttcctt cgttataata
      961 aaccatggat caaacccgaa atgagactgt agccaaggta gttgctttat gtaaacgacg
     1021 ggggattgtt tttccaaatt cagaaattta cggcggcatt caaggatttt acgattatgg
     1081 tcccctcggc actgccatga aacaaaattg gaaaaatatt tggtggcagg aaaatgtagt
     1141 ccgtcgccac gatgttcaag gtattgacgg cgctatcatt actaatcctc aagtctggca
     1201 ggctagcggt cacattaaaa attttactga catagaagat ggtaaaaaat ttaacattat
     1261 gtttgaaact gaggttggga tcaaaaaagg tgataaaata cacacttatt tgagaggcga
     1321 ggcttgtcag gacatctatc ttaattttaa aaacgtggtt gattccaccc gggttaaaat
     1381 ccctttcggt attgctcaaa tcggtaaagc tttccgaaac gaaattactc ccaaaaattt
     1441 tctctatcgc actcgggaat ttgaacaatg ggacatcgaa ttttttattc atccaaaaga
     1501 tgaaaaattt tggtttaact attggcaaaa aaatcggttg gaattctatc gacaatattt
     1561 cagtcaaact tcaaaattaa gattgcgccg acatgaaaag aatgaattag tcttttacgc
     1621 caaggaggct tttgatattg aatatcaagc tccatggggt tgggctgaaa tggaaggtat
     1681 tcacaatcgg ggtgactatg atttaaaaca acatagccaa tttagccatc aagatctgca
     1741 atatcttgac caacaaagcc atgaaaaata cttcccttat ataatcgaat cctccggagg
     1801 agttgatcgg agctttttct tcctcatcct cgatgcttat caggaagaaa aattaaaaaa
     1861 cggcgaaacc cgaatagtct taaaaattca tcctaaaatt gctgcttata aaatagctgt
     1921 tattccctta gctgccaata aaccagagtt aattgatctg gccgaaaaaa tttacagccg
     1981 gctcagtctt aaatatgctg ttgattttga tgattccagt aatattggta aaaaatatcg
     2041 ccgtcaggat gaaattggca ccccctggtg tttaacggtt gattttcaaa ctcttcaaga
     2101 taaaacagtt actcttcgcg atcgcgataa cttaaatcaa attcgcctga aaattgagga
     2161 agttgacaat tggctcgaaa ggcagttaaa taagccaaga tgaaaaaact ttttcttgtt
     2221 ttgataagtg cctttttatt atttggctgt ggcctcaaaa aaacaacacc aacagttacg
     2281 ccgacaccag cctcgactac ctttgaacta acggataaag aaatgcccga aatcaattta
     2341 acagctagtg atgacggtca ttggttaacc ttaaaaatca ataaaatacc gtcatctatc
     2401 gtcaatattg aatacgaatt gctttatcgg gcctcagaga gtggtctgga aatcgagaaa
     2461 ggtttaggtg atacccttaa agtcgaaggc cgtagtttgg aacgaaaatt actattggga
     2521 accgaaagtt gtaccaacgg ttgtaaatat aaatttgatg aaaatgttac tggtggtgat
     2581 ttaaacttaa ctcttattaa tcaacaaggc caaactgcca cgattcaaaa attttttacc
     2641 ctagttaaag ataaaaaaag caaaaaaata gtggccactc ttaccgatgc ctcttcatca
     2701 gccagtcctg ctcaaggaaa taactagact ttttcaacct gggccgggca agatctttat
     2761 cgacgccact ctgggtaatg gtggtcatag tttaaaatta cttcaggcag gagcaaaagt
     2821 ttacgctttt gaatttgatc ctgaaaatat taaggttgtt caaaaaagaa ttcatcaccc
     2881 taatttaacc atcatcaacg ataacttttc tcacattaac cattatttta attttaaggt
     2941 taatggtatt ttatttgatc ttggtttatc gattaatcag caaaaggctc aaggtagggg
     3001 attttccttc aatgatgctc tctcattgga tatgcgctta aatccccaac aacaaacttt
     3061 aactgctcag aaaatcgtca atacgtattc ccaaaaggaa ttatttgacc tcttttccaa
     3121 atacgctcag gaaaaaaaca gtcatttaat tagtcaacta attattaaaa atcggccttt
     3181 taataat
//
LOCUS       DAOOIE010000060         3178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_23517_length_3178_cov_3.054755, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000060 DAOOIE010000000
VERSION     DAOOIE010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23517_length_3178_cov_3.054755"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="PK131_02090"
     CDS             complement(<1..721)
                     /locus_tag="PK131_02090"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44943.1"
                     /translation="MEAAAKQIMQRVIDAGGEIYLVGGAVRDIISNRQVKDWDFTTNL
                     PPEAILKIFSRQSFYNNQFGTVSVIGPNKQIFEITTFRTEIGYQDKRHPDQICWGKSL
                     EEDICRRDFTINALAMDLNGKIYDFCQGENDLKNKLIRAVGNPDQRFEEDALRMMRAV
                     RIASELGFSIEETTLVSIKKNAHLIKEISAERIRDELFKILLAPFNTEGIILLKNSGL
                     LKEIIPELLEGVEVTQKGHHIY"
     gene            complement(884..1150)
                     /locus_tag="PK131_02095"
     CDS             complement(884..1150)
                     /locus_tag="PK131_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011394431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPD44944.1"
                     /translation="MKKLFVGNLSYQMTDDQLKAIFAPYGSVTSANIVKDKFSGRSKG
                     FGFVEFEKDDEAAAAMQALDGSMQDGRNIAVKEAKPRSEEPKMN"
     gene            complement(1181..2254)
                     /locus_tag="PK131_02100"
     CDS             complement(1181..2254)
                     /locus_tag="PK131_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44945.1"
                     /translation="MTKESVYEQGEILKIQKSPLGQLDQLISPEKTPILDLLSEEIGI
                     KTDFPQKQVVCFTAENSQIIEIISKLGIKIDSAATIIEDEVSNLGILVSFKQNYLAFD
                     FKNVRGIEEIIMLNRKYNQPEEREKVDKRVSLKTKEEIKKNPNEIIYNLEKLGQSIDK
                     LTDNPRTILEQIKEKQVDRKTFETYRKIANEDPILQLAARIKKIDENNFIGKELAKFR
                     NKIFKNRYDLLVDFTRDPTITASQQIELFKKINQYRRFYLEFIPDEKMLENLAVELLK
                     NRAAVRQYQLLKMQAEICKDKIEKTIKGIEFNSATRIEGVNIGGTKNGYSHFSLDETA
                     VAKTYAALLFESAMGIRATEKLL"
     gene            2292..>3178
                     /locus_tag="PK131_02105"
     CDS             2292..>3178
                     /locus_tag="PK131_02105"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPD44946.1"
                     /translation="MRKIKLKSNKIITVDTSVKELITILLKNRQILSKDAREFLFPPY
                     PQPSLAGLIRAVARIKKAIRNKENILVYGDYDVDGLTATAILWQVLNKLGAQVWPFIP
                     HRQLDGYGFKAQSFYRFQQEKGVKFNLVITVDNGIVAGREFKKLKDVEVIVADHHLPD
                     DKLPEVYSIIHSTKLSGSGLAWVLASNLDKKADLGLAALGTVADCLPLTGVNRQIVVH
                     GLKKLSTDPCPGLKQLLSVSAVRKAEISAYELGFVLGPRLNAVGRLADPTDGLRLLCS
                     RSENQAAKYAAILDGRNLER"
BASE COUNT          907 a          604 c          564 g         1103 t
ORIGIN      
        1 cataaatatg atggcctttt tgagttactt cgactccctc taaaagttcg ggaataattt
       61 ccttaagtag tccggaattt tttaaaagaa tgataccctc ggtattaaat ggagctagaa
      121 ggattttaaa gagttcgtct ctaatccttt cggctgaaat ttctttgatt aaatgagcat
      181 tttttttaat tgacaccaag gtagtttcct caatagaaaa gcccagttca ctggcaattc
      241 taaccgctct catcattctt agagcatcct cttcaaatct ttgatcaggg ttaccaacag
      301 cccggatcag tttattttta agatcatttt ctccctgaca gaaatcgtaa attttgccgt
      361 tcagatccat cgctagagcg ttaatagtaa aatctcgtcg acagatatct tcttcaaggc
      421 tttttcccca acagatttga tctggatgtc gtttatcttg atagccgatt tcagttctga
      481 aagtggttat ttcaaagatt tgtttattgg ggccaataac actaactgtt ccaaattgat
      541 tattgtaaaa gctctgacgg gagaaaattt taagaatagc ctccggaggt aaattggtgg
      601 taaaatccca atcttttact tgacgattgg aaataatatc tcgaacggct ccacctacga
      661 ggtaaatttc tccaccagcg tcaataaccc tctgcattat ttgtttagcc gccgcttcca
      721 tgccttaatt ttaccttaaa atagatccgc tagccaccgc cctttaacaa agacctataa
      781 tataatataa ccaaatatta gaagcaagaa ttaattttga atttaaaatt ttaaacaaaa
      841 ccccccgata aaatcggggg gttttaaaag accaaaataa tttttaattc attttgggtt
      901 cttcggaacg tggtttagct tccttgacag caatatttcg accatcctgc atactgccat
      961 ctaaggcttg catagcagcg gcggcttcat catccttttc aaattcaaca aaaccaaatc
     1021 ctttgcttct gccgctgaat ttgtctttaa caatgttagc tgaggtaaca ctgccataag
     1081 gagcgaagat ggctttaagc tgatcgtctg tcatttggta ggaaagattt cccacaaaaa
     1141 gtttcttcat actacttgga ctaaaaaatt aacatcccta ttataacagt ttttcggtag
     1201 ctctgatacc catagccgat tcaaacagga gagcagcgta agttttagca acggctgtct
     1261 catcaagact aaaatgagaa tagccgtttt tggttccccc aatatttact ccctcgatgc
     1321 gtgtagccga attaaactcg attcccttaa ttgttttttc tattttatct ttacatattt
     1381 ctgcctgcat ctttaacagc tgatattgcc tgactgctgc ccgatttttt aataattcaa
     1441 ccgctaaatt ttccagcatt ttttcatctg gaataaattc taaataaaac cgccgatact
     1501 gatttatttt tttaaataat tctatttgtt gggaagcagt aatagtagga tcacgagtga
     1561 aatcaaccag aaggtcatat cgatttttga aaattttatt tctaaacttt gccagttctt
     1621 ttcctataaa attattttca tcaatttttt ttatcctcgc ggctaattgc aagattgggt
     1681 cttcattggc aattttccga taagtttcaa aagtttttct atcgacctgt ttttctttta
     1741 tttgctccaa aatggtccga ggattgtcgg ttaatttgtc gatcgattgt cctaactttt
     1801 ctagattata aataatttca ttagggtttt ttttgatttc ttctttagtt ttaagactga
     1861 ctcttttatc cactttttcc ctttcttccg gctgattata ttttcgattt aacataatta
     1921 tttcttcaat ccctctgacg tttttgaaat caaaagctaa ataattttgt ttaaaggaga
     1981 ctaaaattcc caaattgctt acttcatctt cgatgatagt cgcggctgaa tcaattttta
     2041 ttcctaattt gctaataatt tcgataattt gactgttttc ggcagtaaaa cagacaactt
     2101 gtttttgggg aaaatcagtt ttaattccaa tctcttctga aagtaagtct aaaatcggcg
     2161 ttttttccgg gctgattaat tgatctagct gaccgagagg acttttttga attttgagga
     2221 tttccccctg ttcgtaaaca ctttctttag tcattagttt ttctgtaggt gaagcgatta
     2281 tactatagct aatgaggaaa ataaaactta aatcaaacaa gataattact gtcgatactt
     2341 ccgttaaaga attaataact attcttttga aaaatcggca aattttatct aaagatgcta
     2401 gagaatttct ttttcctcct tatcctcaac cgagcctagc cggtttaatt cgagccgttg
     2461 cccgaattaa aaaagcgatt aggaataagg aaaatatttt agtttatggt gattatgatg
     2521 ttgacggttt aacagcaacg gctattttat ggcaagtatt gaataaactg ggggctcagg
     2581 tttggccctt tattcctcac cgccagcttg atggctatgg ttttaaagcc cagtcttttt
     2641 atcgatttca gcaggaaaaa ggagttaaat ttaatctcgt gatcaccgtg gataatggta
     2701 ttgttgccgg cagggagttt aaaaaattga aagatgttga agtaattgtg gccgaccacc
     2761 atctccccga tgataaatta cccgaagttt acagtattat tcattcaacc aagctttccg
     2821 gcagcggtct cgcctgggtt ttagccagta atttggataa aaaagctgat ttaggcttgg
     2881 cggctttggg aacggtagcc gactgcttac cacttaccgg tgttaatcgg caaatagtgg
     2941 ttcatggttt aaaaaaatta tcaacggatc catgtccggg gttaaagcaa ttactgtcag
     3001 tttcagctgt tcgaaaagca gagatatcag cttatgaatt aggttttgtt cttggtccgc
     3061 gattaaatgc cgtcggtcgt ctagctgatc caactgacgg tcttcgttta ctttgcagcc
     3121 ggagtgaaaa tcaagcggct aaatatgcgg ctattttaga tggtcggaat ttggaacg
//
LOCUS       DAOOIE010000061         3169 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_23597_length_3169_cov_3.359987, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000061 DAOOIE010000000
VERSION     DAOOIE010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23597_length_3169_cov_3.359987"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="PK131_02110"
     CDS             complement(<1..860)
                     /locus_tag="PK131_02110"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPD44947.1"
                     /translation="MKKRRNIKHFITGLLLGVVIVGAIWGGIFWERTKNKVDNGVIIS
                     KKEIIDEESVVTRVVEQVTPSVVTVSISKTQVINFDPFWGLDFFNEFFGQPRRDQAQT
                     KKIEQDIGTGFIISSDGLIVTNKHVVADTEAKYRVIIGKDEIVDVKNIYRDPLTDLAI
                     LKIDKTGLKPLKMGDSDSLKVGQTVIAIGTALGEFRSTVTKGVISGLGRGITAGSVFE
                     GMEKLDNVIQTDAAINPGNSGGPLLNSSGEVIGVNVAVSAQGENIGFALPINIIKESI
                     ENFRATGEFDR"
     gene            complement(865..2031)
                     /locus_tag="PK131_02115"
     CDS             complement(865..2031)
                     /locus_tag="PK131_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPD44948.1"
                     /translation="MKFWRRLIIDFLVLFIVIRFSYNYGRWQGTGGLDLSLMRQVKNE
                     LEDKYLDKTKINDNEMKYGAIRGLVASLGDPYTVFLSPKENKGSNEDLAGQFGGVGIS
                     LGYKDKTLAVMAPLPKSPAEKAGILAGDLILKITDEKKGITRETNEISLQEAVSLIRG
                     EVGTEVTLKLYREGNEDTFDVTLKRDNIMVAGVELEWLEDQKIAWIKLSKFTDSLLKE
                     WPMVVDEILSVKTSLDGKNFKGIILDLRNNPGGYLTASVLVGSDFINEGVIVKQESRH
                     GATIDYQVDPSRKKLLDDKLVVLVNGGSASAAEILAGALQFYQRAELVGEKTFGKGTI
                     QEPENFSDGSGIHITIAKWLLPDSRNIHDEGVSPDIEVKGDKAQLEKAKAILLK"
     gene            complement(2028..2777)
                     /locus_tag="PK131_02120"
     CDS             complement(2028..2777)
                     /locus_tag="PK131_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44949.1"
                     /translation="MRSTVRTKWIIRIGPKAKIKCQGKEMVDRGEVILEESNEVFRNY
                     DASVVLSRLPKEKIEALKRSWRGKDVKRGDLIGEVGGIIKKKIFAPVDGKLYDIDEFY
                     NLVIKVEEKNKRIVRSPVRAKVGEISQKKVLLEFDARVIRGQGVVTGRTWGDSDLKEY
                     SAMSDLNYASEGRIILVDNPDQTFITKAEVVGVVGLVVRTIGEVEADIPILKIEPSNW
                     EALLKMGEKNRLYRTFLNTKSERLLVVEDWE"
     gene            complement(2793..3023)
                     /gene="rpsR"
                     /locus_tag="PK131_02125"
     CDS             complement(2793..3023)
                     /gene="rpsR"
                     /locus_tag="PK131_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006300650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPD44950.1"
                     /translation="MLKKGIKKRRIKEQVCSYCQSKTEPSWSDFEKLATFLTNRGRIK
                     SNEMTGLCAKHQRGVARAIKRARYLALLPFVA"
BASE COUNT          916 a          795 c          426 g         1032 t
ORIGIN      
        1 cggtcaaatt ccccggtggc tctaaaattc tcgattgatt ctttaataat attaatcggt
       61 aaagcaaaac cgatattttc cccttgagca gaaacggcca cattaacccc gatcacttcc
      121 ccactcgagt ttaataaggg tccgcccgaa ttacccgggt taatggccgc atcggtctga
      181 atgacattgt cgagcttctc cattccctca aaaactgacc cggcggtaat tcctctacct
      241 aaaccagaaa tcaccccttt agtcactgtt gatcgaaact cacccaacgc cgttccaatc
      301 gcaattaccg tctggccaac cttcaaacta tcagaatcac ccatttttaa tggcttcaaa
      361 ccagtcttat caatttttaa aatagctaaa tcagtcaagg gatctcgata gatatttttg
      421 acatcaacta tttcatcttt tccgataata accctatatt tagcctcggt atcagccaca
      481 acatgtttat tagtcactat cagaccatca ctactgataa taaaaccggt gccaatatct
      541 tgttcgatct ttttagtttg agcttgatca cgccgtggtt gtccgaaaaa ctcattaaaa
      601 aaatctaatc cccagaaagg atcaaaattg atcacttggg ttttagaaat agaaacagta
      661 accacactag gggttacttg ctctaccacc cgagtaacta ccgattcttc atcaattatc
      721 tctttcttac tgataatcac accattatca accttatttt ttgtcctttc ccaaaatatt
      781 cctccccaaa ttgccccaac aataactact cccaatagta aaccggttat aaaatgtttg
      841 atgtttcttc tttttttcat aagactattt taatagaatg gccttggctt tttcaagttg
      901 ggctttatcc cccttgacct ctatatcagg acttaccccc tcatcatgaa tattacgact
      961 atcaggtaat aaccacttgg caatagtaat atggattcct gaaccatcac tgaaattctc
     1021 tggttcttga atcgttccct taccaaatgt tttttctcct actaattctg cccgttggta
     1081 aaactgcaaa gcaccagcta aaatctcagc agcactggcg gaaccaccgt taactaaaac
     1141 cactaatttg tcatccagga gttttttccg agagggatct acttgataat ctattgttgc
     1201 cccgtgtctt gactcttgtt taacaataac tccctcatta atgaaatcgg aaccaaccaa
     1261 cacactggca gtcagatagc cgcccggatt attccttaaa tccagaataa tacccttaaa
     1321 atttttgcca tctagtgaag tttttactga caaaatctca tctaccacca ttggccactc
     1381 ttttaataaa gaatcagtaa atttagataa ttttatccat gcaatttttt gatcctccag
     1441 ccattctagc tctactccgg caaccataat attatctcgc tttaaagtaa catcaaaagt
     1501 atcctcatta ccttcccgat ataatttaag agtaacctca gtacctactt ctcccctaat
     1561 taagctaact gcttcctgca aagatatttc gttagtttcc cgagttattc cttttttttc
     1621 atcggtgatt tttaaaatta aatctccagc taatattccc gccttttctg ctggcgattt
     1681 tggcaaagga gccataacag ctaaagtctt atctttatat cccaaagaaa ttcctacgcc
     1741 cccaaattga ccggccaaat cttcattaga acccttgttt tctttgggtg ataaaaaaac
     1801 tgtgtaaggg tctcccaacg aggcaaccaa acctcgaatg gccccgtatt tcatttcgtt
     1861 gtcattaatt ttggtcttat ctaaatattt atcctccaac tcgtttttaa cctggcgcat
     1921 taaggacaaa tccaaaccac cagttccttg ccatctacca tagttataac taaaccgaat
     1981 aactataaac aagactaaaa aatcaataat aagccgccgc caaaacttca ttcccagtct
     2041 tccacaacta ataatctttc cgacttcgta ttgagaaaag tcctataaag acgatttttt
     2101 tcacccatct ttaaaagggc ttcccaattg ctcggttcaa tctttaaaat aggtatatca
     2161 gcttcaactt caccaattgt tctaacaacc aaaccaacta ctccaacaac ttctgcctta
     2221 gtaataaaag tttggtcagg attatcaacc aaaattatcc tgccttcact ggcataattt
     2281 aaatcgctca tagccgaata ttcctttaaa tcactatctc cccacgttct cccagttaca
     2341 acaccttgac ctctgataac tctggcatca aattccaaaa gtactttttt ttgactaatt
     2401 tctcctactt tagccctaac tggtgaccga actatccttt tatttttctc ctctactttg
     2461 ataaccaaat tatagaattc atcaatatca tataatttac cgtcgacagg ggcaaaaatt
     2521 ttcttcttaa taataccgcc aacttcacct attaaatccc cccttttaac atctttacct
     2581 ctccaactcc tttttaaagc ctcgattttt tctttgggta atctcgacag aactactgaa
     2641 gcatcataat ttcgaaaaac ttcattactt tcttctaaaa ttacttcacc acggtctacc
     2701 atctcctttc cctgacattt tattttggct tttggaccga tccgaataat ccacttggtt
     2761 cgaacagtcg atctcatgat tagattatat atttaagcaa caaagggtaa aagagctaga
     2821 tatctcgctc gcttaatagc ccgagctacg cctctttgat gtttagcaca taatccggtc
     2881 atttcatttg actttattcg accccgattg gtcaaaaaag tagccaattt ctcaaagtcg
     2941 ctccaacttg gctcagtttt cgattgacaa taactacaca cctgctcttt tatccgtctt
     3001 ttttttattc cttttttcag catatttttt taatcctaaa atggcatttc ctcatcgttg
     3061 gccggcttag aattttcctc agccggtttg gccgtggtgg tggtgtcggc agttgcagtt
     3121 gctgattcgt tagtctcagc tggttcaata gcccccaaat catcaggaa
//
LOCUS       DAOOIE010000062         3123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_24019_length_3123_cov_3.871252, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000062 DAOOIE010000000
VERSION     DAOOIE010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24019_length_3123_cov_3.871252"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="PK131_02130"
     CDS             complement(<1..566)
                     /locus_tag="PK131_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44951.1"
                     /translation="MTEKRENETASTLFEKNFVFRGSEEECCWIGRKEYSEKVDDVWS
                     DIVEENEKGPIAAGEKNPEELRVLLKMVRKIEPFESRNNLLFVLNDRMIEGIKSTNEA
                     GIKREYAILVDENLSFLGSRDQKEFSDPDWARCWAAEDFLDMAEIEKKVSGKLEEESR
                     PCNWEVWETWKTWENWETIVGILRLIEE"
     gene            complement(658..2370)
                     /locus_tag="PK131_02135"
     CDS             complement(658..2370)
                     /locus_tag="PK131_02135"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HPD44952.1"
                     /translation="MKKFQKTFSQGNDLLRIISLGGFGDVTQNMFVYELAPQGDFSRS
                     QIIIVDCGTGFPEEDTFGVDLQIPDTTYLQDKKDRILGMFITHGHEDHIGAIRFVLNL
                     LGNKFPIYAPKLAAAFIESRLAEFNLHCRLNLYNEGQLINCGPFTIEPVRVTHSIPDT
                     FHLAIHSPLGICYHGSDFKFDLFPLDGQVTDLAHIARLNSHQQVLCLLSDCLGADHEG
                     YSPSESSIADNFKREIVSAKGRVLVTSISSNIVRWGQAIESAKSCGRKIVLVGMSVEK
                     AINIARDLGYLKLQPSDIIPLESHKNFPDNKLLFLVAGFSAQPDSALSKIVMGKHKIK
                     IKNTDRVIFSSPDYIPGTTTDIYNLIDTLSRLGAEVAYSEKEELHVTGHGYQHEQAIL
                     INLLKPKYLLPIGGNFRHIRSYQLMAKKLGYRDEQIITPEFDQAITFSQSGGVSLTYR
                     VPLKKVLIDGLGIGDVGLTVLRDRKTLAEDGMFSVVLLVNKETGELFKEPVILSRGFV
                     FVKDNPDLMKYLKIEAANKFKQTTSKPANFEFIRTEIQAHLEKIIFDKTGRQPMVLPL
                     VIEV"
     gene            complement(2374..2949)
                     /locus_tag="PK131_02140"
     CDS             complement(2374..2949)
                     /locus_tag="PK131_02140"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014734464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPD44953.1"
                     /translation="MTASKSQQLTYISSLINQCRKCHLYQDATHPVPGSGNPEAKIVF
                     VGEAPGFFEDKSGLPFVGNAGKLLDKLLASIDISRKDVFICNILKHRPPLNRDPLPEE
                     IQACTPFLKAQLKVLQPKLIVALGGVAMNYFFPGELISRIRGQIKEITWEGMNLTIIP
                     VYHPAAALRNGAMLLTLEEDFQTIGNFLIFN"
BASE COUNT          982 a          684 c          554 g          903 t
ORIGIN      
        1 ttttcttcta ttaatctcag tataccgact atggtttccc agttttccca ggttttccag
       61 gtttcccaga cttcccagtt acacggtcga ctttcctcct ccaatttccc gcttaccttt
      121 ttctctatct cagccatatc aagaaagtct tcggctgccc agcaacgagc ccaatcagga
      181 tcagaaaatt ctttttgatc tctgctacct aaaaaactta aattttcatc gactaaaata
      241 gcatactccc ttttaatgcc tgcctcatta gtcgatttta ttccctcaat catcctatcg
      301 tttaaaacaa acaataaatt atttctcgat tcaaatggct ctatttttct aaccattttg
      361 agtaaaactc tcaactcttc aggattcttt tctcctgccg ctattggacc cttttcattc
      421 tcctctacaa tatcgctcca aacatcgtca actttctcac tgtattcttt acgaccaatc
      481 caacaacatt cttcttcact ccccctgaaa acaaaattct tttcaaacaa agtagaagcg
      541 gtttcatttt ctcttttttc agtcatgtga cgtctatata tcataataga acaaaaaagt
      601 caacacaagg ttttccatgt agagacgtcc gcctaggcgg atggggggat ttctaattta
      661 gacttcaatc accagcggca gaaccatcgg ctggcggccg gtcttatcaa aaataatctt
      721 ttctaaatgg gcctgaattt cggtccgaat aaactcaaaa ttagctggct tactggtagt
      781 ctgtttaaat ttattggcag cctcaatttt aagatatttc attaaatcag gattgtcttt
      841 aacaaaaacg aagccgcggg aaagaattac cggttcttta aataattcac cggtttcttt
      901 attgaccaat aaaactactg aaaacatccc atcttcagcc aaagttttcc gatcccgcaa
      961 aaccgtcaaa ccaacatcgc cgatacccaa accgtcaatc aaaactttct ttaacggcac
     1021 ccgataggtt aaactgacac cgccggattg actaaaagta atagcctgat caaattccgg
     1081 agtaataatc tgttcatccc gataacccaa ttttttagcc attaattgat aacttcttat
     1141 atgacgaaaa ttaccgccaa taggcagcaa atattttggt ttaagcaaat taattaaaat
     1201 cgcttgttca tgctgataac catggccggt gacatgaagc tcctcttttt cactgtaggc
     1261 tacttcggcg cctaatcggg aaagggtatc aattaaatta taaatatcgg tagtcgtccc
     1321 tggaatataa tcaggggaac taaaaatcac ccgatcggta tttttaattt taatcttatg
     1381 cttgcccata actatcttgg ataaagctga atctggttga gctgaaaagc cggcgactaa
     1441 aaaaagtaat ttgttgtccg ggaaattttt atgactttct agaggaataa tatcgctggg
     1501 ttgtagttta agataaccca aatcacgggc gatattaatc gccttttcca ccgacattcc
     1561 cactaaaaca atttttcggc cgcaactttt ggcagattca atggcctgtc cccagcggac
     1621 aatattactg gaaatagaag ttaccagcac ccggcctttg gccgagacaa tctcccgttt
     1681 aaaattatcc gcaattgaac tttcggatgg actgtaaccc tcatgatcag ctcccaaaca
     1741 atcagacagc aaacacaaaa cctgttgatg gctgttaaga cgggcgatat gggctaaatc
     1801 agttacctgg ccatcgaggg gaaaaaggtc aaatttaaag tcagatccat gataacaaat
     1861 acctaatggt gagtgaatag cgagatgaaa agtatcggga atagaatgag tgacccgaac
     1921 cggctcaatg gtaaatggac cacaattaat taattggccc tcgttgtaaa gatttaatcg
     1981 gcaatgaaga ttaaactcag ccagccggga ttcaatgaaa gccgccgcca atttaggcgc
     2041 ataaatagga aatttattgc ctaataaatt taagacaaaa cgaattgctc caatatgatc
     2101 ttcgtgacca tgggtaataa acatacctaa aatacggtct tttttatctt ggagataagt
     2161 cgtatcagga atttgaagat caaccccgaa agtgtcctct tccggaaaac cagtaccgca
     2221 gtcaacaata ataatctgac ttcggctaaa atcgccttga ggtgctaatt catagacaaa
     2281 catgttttgg gtcacatcgc caaaaccacc gagggaaata attctcaata aatcatttcc
     2341 ttgagaaaaa gttttttgaa attttttcat aaattaatta aaaattaaaa aattaccaat
     2401 agtttgaaaa tcttcttcta aagttaataa catggcccca tttcggaggg cagctgccgg
     2461 gtgataaacc ggaataattg ttaaattcat tccctcccag gttatttctt taatttgccc
     2521 tctaattcgg gaaattaatt cgccagggaa aaaataattc atcgccactc cgccgagagc
     2581 cacgatcagc ttgggttgta aaactttaag ctgggctttt aaaaaaggcg tacaagcttg
     2641 aatttcttcc ggcaaaggat cccgatttag cggcggccgg tgtttaagaa tattacaaat
     2701 aaacacatct tttcttgaaa tgtcaataga ggccaaaagt ttatcaagta atttaccggc
     2761 attaccaaca aaaggcaggc cgcttttgtc ttcaaaaaag ccgggggctt caccgacaaa
     2821 aacaatttta gcttcaggat tacccgaacc aggaactgga tgagtagcgt cctgataaag
     2881 atggcattta cggcattgat ttattagaga agaaatatag gttaattgtt gagatttaga
     2941 agcggtcatt ggctaagaaa ttgcttaata ttggtttcgt cgatagtaat cggactggca
     3001 gtttgacggg aaaaaattaa caaagccgcc cggcgggcaa tcgcttccag atttctcttc
     3061 aaagtcctaa ccccggcgtc aaaacccaaa ggtcggataa tcagcggcca aacagatgga
     3121 cta
//
LOCUS       DAOOIE010000063         3096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_24311_length_3096_cov_2.784939, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000063 DAOOIE010000000
VERSION     DAOOIE010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24311_length_3096_cov_2.784939"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1571)
                     /gene="groL"
                     /locus_tag="PK131_02145"
     CDS             complement(<1..1571)
                     /gene="groL"
                     /locus_tag="PK131_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258594.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPD44954.1"
                     /translation="MAKQLKFKEDARQALLKGIDTLSNAVVTTLGPRGRNVAIDRKWG
                     SPSVIHDGVSVAKEIDLKDPYENMGAQLMKEAASKTNDVAGDGTTTATLLAQAIANKG
                     LQNVTAGSNPMMLRRGLEKGLEVILQELDEMKKEIKIDDKESIEQVATISAGDETIGK
                     RIAEAVAKVGKDGLITAEEGKGMGIETKETSGMEFDQGFLSPYFATNTETMEAIIDHP
                     YLVITDKKITSIQEILPFLEKLVKLTKNFVIIADEIEGEALATLVVNKLRGTFSVLAV
                     KAPGFGDRRKAMLEDIAILTGGRVISEDVGAKFENIDPREYCGRADSVTADKDKTRII
                     GGAGNQAEVEARVSQLRTQMAKETSDYDKEKLQERIAKLIGGAIVLQVGGATEVEMKE
                     KLERVKDAIDATKAAVEEGILPGGGVALLKASFALEKVKVATEEEQIGLNILKYVLEQ
                     PIRKLAENSGEDAGFVLNKIKEALLADKKSDFGFNAQTGEFGSMVKFGILDPAKVTKS
                     ALINAVSIGIMVLTT"
     gene            complement(1611..1907)
                     /locus_tag="PK131_02150"
     CDS             complement(1611..1907)
                     /locus_tag="PK131_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPD44955.1"
                     /translation="MKKATIQPVAGYILVKPQKQERTTASGIVIPDSHEEKPQQGEVL
                     AIGAPIFEHGHEIKAPCQVGDIVIYREWGGKEYKLNDVDYLILKFDDIMATINN"
     gene            2141..>3096
                     /gene="eno"
                     /locus_tag="PK131_02155"
     CDS             2141..>3096
                     /gene="eno"
                     /locus_tag="PK131_02155"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012341.2"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPD44956.1"
                     /translation="MAKIIEIDAREILASGGEPSLEATVKLDNGSVGEASISYGASAG
                     TKEAFVLFDGDKSRYHGKGMLKAKANILEKIAPAIMGMEAADLRTIDKKMIELDGTEN
                     KANLGANAILAVSMAVARAESNSQKLELYQYLRNLWQIEGEITLPKPMMVMIEGGKHA
                     DNSTDMQEFLVSAMSNRSAMENVRMEMEIYKALKDILKAEGLSTNVGNEGAFAPAGLK
                     SNETPLAYLTKAIERAGYRAGVDAGISIDAAASELYNYELKNYELRIENKQLTSEEMI
                     NYLKPWVEKYPIVSMEDMLSEFDWENWPKLVEYFGNNILIIA"
BASE COUNT          930 a          665 c          594 g          907 t
ORIGIN      
        1 tcggtcgtca aaaccattat accaatagaa acggcattaa ttaatgctga tttggttact
       61 ttagccggat caagaatccc aaatttaacc attgagccaa attcgccggt ttgggcatta
      121 aaaccaaaat cagacttttt gtcggctaaa agagcttctt taattttgtt caggacaaaa
      181 ccagcatctt cacccgaatt ttcggctaat tttctaatcg gttgctctaa aacgtacttt
      241 aaaatattca agccgatttg ctcttcttcg gttgccactt taactttttc caaagcaaat
      301 gaggctttca aaagagctac gccgccgccg ggtaaaatac cttcttccac tgccgccttg
      361 gttgcgtcaa tcgcatcctt taccctttcc aatttctctt tcatttccac ttcggtagcc
      421 ccaccaacct gtaaaacaat agctcctccg attaacttgg caattctttc ctgtaacttc
      481 tctttatcat aatcggaagt ttccttagcc atttgagtcc gtagttgaga tactcgagcc
      541 tcaacttcag cttgattacc agccccacca ataattcttg ttttatcttt atcggcggtt
      601 accgaatcgg cccggccgca atattcccgc ggatcaatat tttcaaattt agctccaaca
      661 tcctcggaaa ttacccgacc accagttaaa atagcaatat cttccaacat cgccttacgc
      721 cgatcgccga aacccggagc tttaactgct aaaactgaaa aggtgcctct taatttatta
      781 accaccaaag tcgccaaagc ttcaccttca atctcatcgg caatgataac gaaattttta
      841 gtcagtttga ctaatttttc caaaaaagga aggatttcct ggattgaagt aattttttta
      901 tcggtaatta ctaaataagg atgatcgata attgcctcca tcgtttcagt attggtggca
      961 aagtagggcg aaagaaaacc ttgatcaaat tccatccctg aagtctcttt agtttcaatc
     1021 cccataccct taccttcttc ggcggtaatt aaaccatctt taccaacttt agccacagcc
     1081 tcggcaattc tcttgccaat agtttcatct ccggccgaaa tagtcgctac ctgctcgatt
     1141 gattccttgt cgtcaatttt aatctccttt ttcatttcat ctaattcttg tagaatcacc
     1201 tctaaacctt tttccaagcc gcggcgcaac atcattggat tagacccggc agttacgttt
     1261 tgaagaccct tattagcgat agcttgggct aaaagagtag cagtggtagt gccgtcaccg
     1321 gcaacatcat tagttttact ggccgcctct ttcattaatt gagctcccat attttcgtaa
     1381 ggatccttta aatcgatttc cttagctaca gaaacaccat catgaataac tgacggacta
     1441 ccccattttc tatcaatagc tacattacgt ccccgaggac ctagagtagt cacgactgca
     1501 ttagataaag tgtcaatacc ttttaacaat gcctgacggg catcttcttt gaattttaat
     1561 tgtttagcca taatttcatt ttctaatttt taatctttaa ttcgtaattt ttaattgttg
     1621 atagtggcca tgatatcgtc aaattttaaa attaaataat caacatcgtt taatttatat
     1681 tccttaccac cccattcccg ataaatcact atatcaccaa cttgacaagg agctttaatc
     1741 tcatggccat gttcaaaaat aggagcaccg atagctaaaa cctccccctg ttggggtttt
     1801 tcttcatggg aatcaggaat aacaataccg gaagcagtgg ttctttcttg cttttggggt
     1861 ttaactaaaa tatatccggc tactggttga atagttgctt tcttcattat tgattataaa
     1921 aattataaac ttaataatag cactctctcc gagtgactgc taatattata ttcaggaaaa
     1981 tcggggtgtc aagaggaaaa aggatcttta tctcaatttg ttaggaacgc gcctaggaca
     2041 aagactgggc gtgttcatag acgaaatcga ttatatccta attaattgcc aagcacgttt
     2101 atcaaaatgg gcgggtgttt ttcctgtata atctatcagg atggcaaaaa taattgaaat
     2161 agacgcccgg gagattttag cctccggtgg tgaaccgagt ttggaggcaa ccgttaaatt
     2221 agacaatggc agcgtcggcg aagcgtcgat ttcctatggc gccagcgccg gcaccaagga
     2281 agcgtttgtg ctttttgacg gagacaaaag ccgttaccac ggcaagggaa tgttgaaagc
     2341 caaagcgaac atattggaaa aaattgcccc ggcaataatg gggatggaag cagcggatct
     2401 acggacgatt gataaaaaaa tgattgaatt ggatggaaca gaaaacaaag ccaatttggg
     2461 ggcgaacgca attttagcgg tcagtatggc tgttgctcgg gctgaatcca atagtcaaaa
     2521 actagagctt taccaatact taagaaattt atggcaaatt gagggagaaa tcactctgcc
     2581 caaaccgatg atggtgatga tcgaaggcgg caagcatgcc gacaacagca ccgatatgca
     2641 ggaattttta gtcagtgcca tgagtaatag atcagcaatg gaaaacgtca gaatggaaat
     2701 ggaaatttac aaagccctga aagatatttt aaaagcagaa ggtttatcaa ccaatgtcgg
     2761 caatgagggt gcctttgccc cagcaggcct caaaagcaat gaaacaccac tagcctatct
     2821 gacaaaggcg attgaaaggg ctggttatcg agccggggtg gatgcgggaa tttctatcga
     2881 tgcggcggca tcggaactgt ataattacga attgaaaaat tacgaattac gaattgaaaa
     2941 caagcaatta acatcggagg aaatgattaa ttatcttaag ccatgggtag aaaaatatcc
     3001 aattgtaagt atggaagata tgctgtcgga atttgattgg gaaaattggc ctaaattagt
     3061 ggaatacttt ggcaataaca tcttaattat tgccga
//
LOCUS       DAOOIE010000064         3090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_24376_length_3090_cov_2.865898, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000064 DAOOIE010000000
VERSION     DAOOIE010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24376_length_3090_cov_2.865898"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="PK131_02160"
     CDS             complement(<1..420)
                     /locus_tag="PK131_02160"
                     /inference="COORDINATES: protein motif:HMM:NF021607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HPD44957.1"
                     /translation="MERFSFKSLLIIIVVLLIGIVGLWQIQRRAKPAEAGWFNESWQY
                     RRSIGISNPSGSNLTDFQVSVNIGTSALIAAGKMKSDCSDIRFTDQNGKLLDYWIEEN
                     NPGCNQTTDTKIWVKLPSLPTSNATAYLYYGNPSATAY"
     gene            complement(584..1741)
                     /gene="serS"
                     /locus_tag="PK131_02165"
     CDS             complement(584..1741)
                     /gene="serS"
                     /locus_tag="PK131_02165"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPD44958.1"
                     /translation="MSKGVPAGQGIFMVDINVLKADYLKILREVEQLRAVKNQIKGKP
                     TAEELAKAKKLKEELKAKEKLLKTAETACQQAILDERNTPAPDVPAGENDKDNVVIKT
                     VGEIPKFDFTPKDHLAIGESLDIIDVKKAGVISGTRFGYFKGLGAELELALMYYAFHK
                     LTKLGFVGMIPPALIKTDIEAKMGYTTGKNLGGDYYLLPEDNLALIASSEHSVVPYHV
                     GETIDSKKLPLKYVNFSACFRREAGTYGQDTRGLFRVHQFNKVEMNVFTAPDDKISDE
                     MCQELLAIQEGIITDLGLPYQIVKNCTGDLPRPNRRMYDINAWFPGQNRYREITSCSN
                     CTDYQAKRLGIKNVHILNATAVSDRFVLAIIENYQKADGTFEIPKILLPLL"
     gene            complement(1856..2443)
                     /locus_tag="PK131_02170"
     CDS             complement(1856..2443)
                     /locus_tag="PK131_02170"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPD44959.1"
                     /translation="MNRDQYLQAVQSQLEKNIFYHSLALEACMGGLYDYLSPLVKGGC
                     PEDRGIWTLAGLIHDIDYSEPYKNDHPNKTAEVLAKYNLKAPDKVLNIIKAHSPQTSG
                     VYPTTAGQWAIFCADSLTGLITATALVIPSKKLAEVKLASVIKRFLKEPRFAAGTRRE
                     EIKMCEKPEGLNIPLEKFIEICLTSMQSISDQIGL"
     gene            complement(2499..3005)
                     /locus_tag="PK131_02175"
     CDS             complement(2499..3005)
                     /locus_tag="PK131_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44960.1"
                     /translation="MTFHDAVLKETGIDTDKITEKDLKIDGIGLGAKLDNFYKEKVRP
                     KIIEPTFVVDYLSEMIALAKRKENNSKYIASFQLLVNGVELLKAYNELNDPVEQAARW
                     NDETALAKKGAENAMQFDQDYIQALEYGLPPVAGWGMGIDRFVQIITNQPCIKDVIFF
                     PAMRSEEI"
BASE COUNT          892 a          634 c          630 g          934 t
ORIGIN      
        1 ataggcagtg gccgaaggat tgccatagta aagataggca gtagcgttag aggtgggaag
       61 agaaggaagt ttaacccaaa ttttagtatc ggtagtctga ttacagccag gattgttttc
      121 ctcaatccag taatccagta atttgccgtt ttgatcggtg aacctgatat cggaacagtc
      181 ggatttcatt ttgccagcgg caattaaagc tgaagtgcca atattaacgc tgacctgaaa
      241 atcggttaaa ttagagccgg aaggatttga aataccgata gatcggcgat attgccaaga
      301 ttcgttaaac catcccgcct cggcgggttt agcacgccgt tgaatctgcc ataaaccgac
      361 aatgccgatt aataaaacaa cgatgatgat taaaagagat ttaaaagaaa aacgctccac
      421 caattacatt atatattaaa cccccttcca gaaacacaat ttctgttttg tcttgctccc
      481 ttgtcaaggg gagctgtccg gaggactgag gggtttaaaa ttggaatttc ttttctcctc
      541 tcccaccaaa tggcgggagg gaaaaaaggt ggctcctcta aagttataaa agcggcagta
      601 aaattttggg gatctcaaaa gtgccgtcgg ctttttggta gttttcaata atggctaaaa
      661 caaaacggtc gctgacggct gtggcattta aaatatggac atttttaatc cctaatcttt
      721 ttgcctgata atcggtacaa tttgaacaac tagtaatttc ccgataccta ttttgtcccg
      781 gaaaccaggc attaatatca tacatgcgtc gatttggccg ggggagatcg ccggtgcaat
      841 ttttgactat ctgatagggg agacctaaat cggtgataat cccctcttga attgccagta
      901 attcctgaca catttcatcg gaaattttgt cgtcaggagc ggtaaaaaca ttcatttcta
      961 ccttattaaa ctggtgaacc cggaaaagcc ctcgggtgtc ttggccgtaa gtaccggcct
     1021 cgcggcgaaa acatgcagaa aaattaacgt attttaaggg taatttttta gaatcaattg
     1081 tttccccgac atgataaggc acaactgaat gctcagaaga agcgattaaa gccaaattat
     1141 cttccggtaa aagataataa tcaccgccta aatttttgcc ggtagtataa cccattttgg
     1201 cctcaatatc ggttttaatc aaagccggcg gaatcatgcc gacaaaacct agtttagtta
     1261 atttatgaaa ggcataatac atcagggcca actccaattc ggcgccgaga cctttaaaat
     1321 agccgaagcg ggtgccggaa attactcccg ccttcttaac atcaataatg tcaagcgact
     1381 cccctattgc caaatggtct tttggagtga agtcaaattt aggaatttca ccgacagttt
     1441 taataactac gttatctttg tcattctccc ctgccggcac atccggcgcc ggcgtatttc
     1501 gttcatctaa aatagcctgt tgacaggcag tttcggcagt ttttaataat ttttctttgg
     1561 cttttaattc ttctttgagt ttcttggctt tggctaattc ttcggcagtg ggcttgccct
     1621 taatctgatt tttaaccgcc cgcagctgct ctacttcccg gagaattttt agataatcag
     1681 cttttaaaac attaatgtcg accataaaaa tcccctgccc tgctggcacc ccttttgaca
     1741 agggggaact tttgattgat taattttaac attaattttt aattttattt ccataaataa
     1801 aacgcaaaaa gttttgctcc cttgtcaagg ggagctgtcc gaagggctga ggggtttaaa
     1861 gtccaatttg gtcggaaatg gattgcatgg aggtcaggca aatctcaatg aatttttcta
     1921 aaggaatgtt aagaccttca ggtttttcac acattttaat ttcctcccga cgggtgccgg
     1981 cggcaaaacg gggttcttta agaaatcttt ttatcactga cgctaattta acctcggcta
     2041 attttttgga aggtattacc aaagccgtag ctgtaattaa gccggtaagg gaatcggcgc
     2101 agaaaatcgc ccactgtcct gccgtagtcg gataaacacc agaggtttgg ggagaatgag
     2161 ctttgattat atttaaaacc ttatcgggtg cttttaaatt atatttagcc aatacttcgg
     2221 cagttttatt aggatgatca tttttatacg gttcggaata atcaatgtcg tgaatgagac
     2281 cggcgagagt ccaaatcccc ctgtcctccg gacatccccc tttgacaagg ggggacaaat
     2341 agtcataaag accgcccatg caagcttcga gggcaagcga gtgataaaaa atatttttct
     2401 ctaattggct ttgaacagcc tgaagatatt gatcacgatt cataaactca actttcaatt
     2461 ttcaaaatcc aatgaatttt aattattaaa ttttaaaatt aaatttcttc gcttcgcatt
     2521 gccgggaaaa agataacatc tttgatacag ggttgattgg taataatttg aacaaagcgg
     2581 tcaatgccca tcccccagcc ggccaccggc ggcaagccat attccagcgc ttgaatatag
     2641 tcttggtcaa attgcatcgc gttttcagca ccctttttgg ccaaagcagt ctcatcattc
     2701 cagcgggcgg cctgctcaac cggatcgtta agctcattgt aagctttcaa taattcgacc
     2761 ccattgacta aaagctgaaa agaagcaata tatttggaat tattttcctt tcttttagcc
     2821 aaagcgatca tctcactgag gtaatcaacg acaaaagtcg gctcgataat tttggggcga
     2881 actttttctt tatagaaatt gtctaatttg gcgccaagac caataccatc aattttgagg
     2941 tctttttcgg taattttatc ggtgtcaata ccggtttcct taagaacagc gtcatgaaaa
     3001 gttatctttt tgattttggt aaaatcaaag cctgatttgt tttcaacctt agttaagact
     3061 taattaatta attcttgggt aaatttcatt
//
LOCUS       DAOOIE010000065         2978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_25651_length_2978_cov_3.674307, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000065 DAOOIE010000000
VERSION     DAOOIE010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25651_length_2978_cov_3.674307"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /locus_tag="PK131_02180"
     CDS             complement(<1..1373)
                     /locus_tag="PK131_02180"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPD44961.1"
                     /translation="MKWNLDGILKENKFDQLYREIETDLEKFKILIADLDPAMSAVKF
                     KDIIDFSEAMANKFSRLAKLPELKLTINSKNQKARLMQIRSENLGLKITEETIKFTHW
                     LKGLNGGLDDDNAQRLFKTISDLEYSLNYSRKAAKYTLSEKEEKIILNKSLNLGGALS
                     DLKDLIETEFQYCVLGKKVKTQAELLTFTSSPKAEERKAAYQALLNKQKENIDKFFVL
                     YQSTVRDWTYEARIRHYSSPIAMRNFDNEIDDDLVDLVLTVTQKNKKIFYPYFMAKAK
                     KLGSAKLNRFDLYAPIDNNPEIKTDLPQAIKLVLNAFNEFSPRFAAAASRIISQNHLD
                     SSPAPGKKSGAFCAPINNEIDPYVMLNYTGRLRDITTLAHELGHSIHQIYAAHHYSAV
                     QSAGLPLAETASTFGEMLVFEKLKKEMLWKKIDDLYATVLRQNYFVLFEKEAYQAINQ
                     GITEKELS"
     gene            1402..2040
                     /locus_tag="PK131_02185"
     CDS             1402..2040
                     /locus_tag="PK131_02185"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPD44962.1"
                     /translation="MITPDFAFEKSLLPARARFVLGIDEVGIGSWAGPVVVGAFLLDL
                     ADFNPDIFIANRIRDSKTLSARQRRRIVDWFKQQGFSFQLFLSRADTIDKKGIRSVLI
                     DSIGRAISFYRGAFDFVLIDGNLKIGNRWLVRSLPRGDACCFSIAAASICAKVYRDRL
                     MIKFARSYTYYDFDHNMGYGTKKHLAGLKAHGICPLHRLSYKPVKFFANHSP"
     gene            complement(2028..>2978)
                     /locus_tag="PK131_02190"
     CDS             complement(2028..>2978)
                     /locus_tag="PK131_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44963.1"
                     /translation="SDIGGRFSAPNTLVFLLPLYFLGGKETKFVQNIWQEYLQAIDRE
                     LVDKMAQAARKIVDSGSEFLAIVLDSYLTEAMENWITQLFQESLGSKLEMWLFHLFKS
                     SGASKLSGFNLKTLVAPLNEVPNYFKLVNLDLKNTNRMVEMMVNFYAAQLLVAFIAYG
                     KRVNFVDQPEVENYKRIMRGSKPGELNLPQKSKIGQIIEETNKVLENKPEIKFVEIVC
                     YWHLDKDKRQKIKEIFQRRLPGREILVFEGSDWNHHSLQAAVKDPRTLFEILIYADYS
                     EYTNQIQSEMGKNNVETLRAIAYSTYKSLKDKALFMSYGE"
BASE COUNT          746 a          602 c          622 g         1008 t
ORIGIN      
        1 gacaattctt tttcagttat tccttggtta atagcttggt aagcttcctt ttcgaagaga
       61 acaaaataat tttgtcggag cacagtggca taaagatcat cgattttttt ccaaagcatt
      121 tcttttttca gtttttcaaa gactagcatt tcaccaaagg ttgaagccgt ttccgccagg
      181 ggtaaaccag ctgattgaac cgccgagtag tgatgagcgg catagatttg gtggatactg
      241 tggcctaact catgggctag cgtagtaata tcccgtaacc tgccagtata atttagcatt
      301 acataaggat caatttcatt attaatagga gcgcaaaaag ctccgctttt tttaccggga
      361 gccggtgaag aatcaaggtg gttttgactg atgattctac tagcagcagc ggcaaaacgt
      421 gggctaaatt cattaaaagc attaagcact aatttaattg cctgtggcag gtcagtcttg
      481 atttctggat tgttgtctat tggggcataa aggtcaaatc gattaagttt agctgagcct
      541 agttttttag ctttagccat aaaatatggg tagaaaattt ttttgttttt ttgagtaacg
      601 gttaagacta aatccaccaa atcatcatca atttcattat cgaagtttct catagcgatt
      661 ggtgatgaat aatgacggat tctggcttca taggtccaat cccggacagt gctttgataa
      721 agaacgaaaa atttatcgat attttctttc tgtttgttaa gtaaagcctg ataagctgct
      781 tttctttctt ctgctttagg gctggaagtg aaagtcaata attctgcttg ggtttttact
      841 tttttaccta aaacacagta ttggaattca gtttcaatta agtcttttaa atcagataaa
      901 gccccgccta aattgaggct cttattaaga ataatttttt cctctttttc tgaaagggta
      961 tatttagctg cttttcgact ataattaagg ctatattcta aatcggagat agttttaaac
     1021 agtctctggg cattatcgtc atccaatccg ccgtttaatc cttttaacca gtgggtaaat
     1081 tttattgttt cttcggtgat ttttaagccg aggttctccg atcggatttg cataagccga
     1141 gctttttgat ttttgctatt gatcgttagc tttagttctg gcagtttggc tagtcggcta
     1201 aacttattgg ccatagcttc gctaaaatca atgatatctt taaatttgac tgccgacata
     1261 gccggatcta aatcagcaat gaggattttg aatttttcta aatcggtttc tatttctcga
     1321 tacaactggt caaatttatt ttcttttaaa ataccatcta aattccactt catttggtca
     1381 attatacctt ataattggtc tatgattaca cctgattttg cttttgaaaa atcgctgctt
     1441 ccagcaagag ctaggtttgt tttgggaatt gatgaagtcg gtatcggttc ttgggccggc
     1501 ccggtggtag tcggggcttt ccttttagat ttggccgatt ttaaccccga tattttcatc
     1561 gctaatcgga ttcgcgactc caaaacttta tccgcccgcc agcgccgccg aattgttgat
     1621 tggtttaaac aacagggctt ttcctttcag ctgtttttgt cccgcgccga cacgatcgat
     1681 aaaaagggaa ttcgatcggt tctgatcgac agcatcggcc gggcgatcag tttttaccgg
     1741 ggcgcttttg atttcgtttt aattgacggt aatttgaaaa ttggaaaccg atggctggtt
     1801 aggagcctcc cccggggcga cgcctgctgt ttttccattg ccgccgcttc catctgcgcc
     1861 aaggtttacc gtgaccggct gatgattaaa tttgcccgta gttacaccta ttatgatttc
     1921 gatcacaata tgggctacgg taccaagaag catttagccg gcctcaaggc ccacggtatc
     1981 tgccctcttc accgtttgtc atataaaccg gtgaaatttt tcgctaatca ctcgccatag
     2041 ctcatgaaca aggccttgtc ttttaggctt ttgtaagtac tataggcaat agccctgagg
     2101 gtttcgacat tattctttcc catttcactt tggatttgat tggtgtattc gctgtaatcg
     2161 gcgtaaatta aaatttcaaa tagagttctt gggtctttga cggccgcctg gaggctgtga
     2221 tggttccaat ccgaaccctc aaaaaccaaa atttctctcc cgggtaatct tctctggaat
     2281 atttctttga ttttctgccg tttgtctttg tctaagtgcc agtagcagac gatttccaca
     2341 aatttaattt ctggcttatt ttccagaact ttatttgttt cctcgataat ttggccaatt
     2401 ttgctttttt gggggaggtt taattctccc ggctttgagc ccctcattat ccttttgtaa
     2461 ttttctactt ccggctggtc aacaaagtta actcttttgc cgtaggcgat gaaggcaacc
     2521 aataattggg cggcataaaa attgaccatc atttctacca ttctgttggt attttttaaa
     2581 tccaaattga ccaacttgaa ataattcggt acttcattaa ggggagcaac cagagttttc
     2641 aagttgaagc cgcttaattt tgatgctcca gacgacttaa ataaatggaa tagccacatt
     2701 tccagcttcg agcctaaaga ttcctggaac agctgggtga tccagttttc catggcttcg
     2761 gtcagatagc tgtctaaaac tatggctaga aattcactgc cgctgtcgac gattttcctg
     2821 gcagcttgcg ccattttgtc aactaattcc cggtcaatgg cctggagata ctcctgccag
     2881 atgttttgga cgaatttggt ctctttcccg ccgagaaagt agagagggag gagaaaaacg
     2941 agggtgttag gggcgctgaa ccggccgccg atgtcgct
//
LOCUS       DAOOIE010000066         2974 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_25695_length_2974_cov_3.107229, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000066 DAOOIE010000000
VERSION     DAOOIE010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25695_length_2974_cov_3.107229"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="PK131_02195"
     CDS             <1..591
                     /locus_tag="PK131_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44964.1"
                     /translation="DSILEYTQFIFYFLAAIGAFRNFLQLKKNKSNKTYSILFLLLSI
                     GLIFISLEEISYGQRIFGWKTPSEIQKVNIQKETNIHNIFSYNINEIVYILISFYGIF
                     SEKIVNIFFKKKKQKLSILIVPRFLLFYFIFLLLVYFDRRFINLNYDTVVNNAFRKYA
                     LWDWLEVGELYLAIAFWAYTKNANQSIKTNESLRIT"
     gene            complement(1096..1169)
                     /locus_tag="PK131_02200"
     tRNA            complement(1096..1169)
                     /locus_tag="PK131_02200"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1135..1137),aa:Gln,seq:ttg)
     gene            complement(1227..1676)
                     /locus_tag="PK131_02205"
     CDS             complement(1227..1676)
                     /locus_tag="PK131_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44965.1"
                     /translation="MTEQIVTRPYVPNSVPTIGIEVQSVNLNKKEFLDLNSLDLNNLK
                     PGELTQIILDQYQQQFGLTDKKPNIYFHLCQKKPDNNENTRSPSLQIFTSDVRLGNLN
                     REGFYRLVVDGRQNDNAKTKKRLKEILRYLRGGVVEIRGIYAGEKIK"
     gene            complement(1685..1757)
                     /locus_tag="PK131_02210"
     tRNA            complement(1685..1757)
                     /locus_tag="PK131_02210"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1722..1724),aa:Glu,seq:ttc)
     gene            1832..2215
                     /locus_tag="PK131_02215"
     CDS             1832..2215
                     /locus_tag="PK131_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44966.1"
                     /translation="MVERDNFREVCRLEEQIDPRKIKAKFGSLFNEAQKSQLFEIIEK
                     KQGNYEITYISLFGTVIAVKGVDFGNKDIVYTLYGGRHEVKLNLLESENGKLKTVSIE
                     LSPEWVQKLRTNENIVECINDLFSL"
     gene            complement(2232..2435)
                     /locus_tag="PK131_02220"
     CDS             complement(2232..2435)
                     /locus_tag="PK131_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44967.1"
                     /translation="MKPAKFDLLGASLIVAFFLALTYAGYLAYKSIDYKILDKLESAP
                     LILPTPIPTSFSTETATPSSLPQ"
     gene            complement(2440..>2974)
                     /locus_tag="PK131_02225"
     CDS             complement(2440..>2974)
                     /locus_tag="PK131_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44968.1"
                     /translation="LAYATPKTKDRHERALSPISPNSWAYNPTVKEYNFDPERAKELL
                     GETDLGQLKIAVADRQLLNTAENIKKSWHDILGIEVETQIVNKIDPQNFDAFLAYGGI
                     LADPDQYLFWHSTQTQTNLTKFNNSRIDKLLEEGRQTFDPIERKKIYQDFQKYLLEEC
                     PAIFLSYPTIYTISRLK"
BASE COUNT         1004 a          445 c          554 g          971 t
ORIGIN      
        1 gactctatat tggaatatac acaatttata ttttattttt tagcagcaat tggtgcattt
       61 agaaattttt tacaacttaa aaaaaataaa agcaataaaa cttattccat attattttta
      121 ttgctttcaa ttggtttgat ttttatatct cttgaggaga tttcttatgg gcagaggatt
      181 tttggatgga aaactccaag tgagatacaa aaagttaata tacagaaaga gacaaatatc
      241 cataatattt ttagttataa cattaatgaa attgtttata tcttaatcag tttttatggg
      301 attttttcag aaaagatagt taatatcttt tttaaaaaaa agaaacagaa attaagtatc
      361 ttaatagttc ccaggttttt acttttttat tttatatttt tacttttagt ttattttgac
      421 agacgcttta taaatcttaa ttatgatact gttgttaata acgcctttag aaaatatgcg
      481 ctttgggatt ggttagaagt gggtgagtta tatttagcca ttgctttttg ggcttataca
      541 aaaaatgcca atcaatcaat taaaacaaat gaatcgctac gaattactta aaccaacagt
      601 attagtataa agtttacata aatattttta gatatctact caaaattacc attgaacctt
      661 taggattagt attcaacccc aagaattgat aatccaatca gaattactaa tcgagagcta
      721 tcaaacccag acattgatat aattactagc ctatatgaac aatagactca gaacaaataa
      781 tcttacagga ctttttttct aaaagtcagc aaaaatatgc taattaaatt tattaacaca
      841 ttagggataa acaaaattgg agtttgttac aagacggaaa gttcgatatt acagtttgcg
      901 gtgctccaat taaataactc ctattaagaa caaacctatt aagaacaaat cagtcgtttt
      961 tgatccacag aaaagagatt cgagcggagg tttttcgaag gtaagaaacc gttatgattc
     1021 tcacttttca aaatagcact ttagaagaaa aacacgggtt attcaaggga gtagggtcac
     1081 taggggtgcc taacctggct gccccgcgtg gattcgaacc acaataaaca gctccaaagg
     1141 ctgctgtcct accattagac gacagggcaa ttaaggtaaa ttttctctat taaaccataa
     1201 atcttgccgt tattccagtc agattttcat tttatttttt caccggcata aatacccctg
     1261 atttccacta ccccaccccg gagatatctt aaaatttcct taagacgttt tttggtttta
     1321 gcgttgtcat tttgacggcc gtcaacaacc agtcgataaa aaccttcccg attaagatta
     1381 ccgagacgaa catcagaagt gaagatttga agactaggtg aacgagtatt ttcgttatta
     1441 tcaggttttt tttgacaaag atgaaaataa atattcggct ttttgtcggt taatccaaat
     1501 tgttgttgat attgatcaag aataatctgg gttaactcgc ctggttttaa attatttaag
     1561 tccaaagaat ttaaatcgag aaattccttt ttatttaggt tgaccgattg aacctcgata
     1621 cctattgttg gtaccgaatt aggtacataa ggtcgagtaa caatttgttc agtcatattt
     1681 caagtgactc cagtgggagt cgaacccacg attacctgga tgaaagccag gtgtcctaac
     1741 cactagacga tggagccgtt aaactggtct tattttaaca gatacaggcc gaacttgata
     1801 tatggcctat agtgtgatat aataaaagta tatggtagaa agggataatt ttagggaagt
     1861 ttgtaggcta gaagaacaaa tagacccgag gaaaattaaa gccaagtttg gaagtctttt
     1921 taatgaagcc caaaagagcc aattattcga gataattgaa aaaaaacagg gcaactatga
     1981 aatcacttat atttccctgt tcggaacagt gatagcggta aaaggtgttg attttgggaa
     2041 taaggatatt gtttacactt tgtacggagg tcgtcatgag gtgaagttaa atttacttga
     2101 atcagaaaac ggtaaactaa agacagtttc gattgaatta tctccggaat gggttcagaa
     2161 attaagaact aatgaaaata tagttgagtg cattaatgac ctttttagtt tatagaataa
     2221 agcaggctaa cttattgagg tagagaagat ggagtagcag tttcggtgga aaacgaggta
     2281 ggaattggtg tcggcaggat taatggggct gattcgagtt tatctagaat tttataatcg
     2341 atacttttgt aggctaaata accggcgtaa gtcaaagcca aaaaaaaagc tacaataaga
     2401 ctagctccta aaagatcaaa tttagctggt ttcatgtttt tattttaatc gagaaatggt
     2461 gtaaatggta ggataagaaa ggaagatggc ggggcattct tccaagagat atttttgaaa
     2521 gtcctgatag atttttttgc gttcaattgg atcaaaagtc tgtctcccct cctcgagaag
     2581 tttatcgatg cgggaattat taaatttagt taaattggtt tgggtttgag tgctatgcca
     2641 aaaaagatat tggtcgggat cagctaaaat accgccatag gccagaaagg catcaaaatt
     2701 ttgaggatca attttattaa ctatttgagt ttctacctcg atacctagaa tatcatgcca
     2761 tgattttttt atattttcgg cagtatttaa aagttgtcta tcagcaaccg caattttcaa
     2821 ttgacctaaa tcagtttcac ccaataactc cttagctcgt tcgggatcga aattatattc
     2881 tttaactgtt ggattataag cccaagaatt aggggaaatc ggtgaaagag ctcgttcatg
     2941 tctatctttg gttttaggag tggcataagc taag
//
LOCUS       DAOOIE010000067         2919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_26330_length_2919_cov_3.456355, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000067 DAOOIE010000000
VERSION     DAOOIE010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26330_length_2919_cov_3.456355"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="PK131_02230"
     CDS             complement(<1..660)
                     /locus_tag="PK131_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44969.1"
                     /translation="MKNKLVFLAAILGLLSCLRLFRPGFYSTQDEMHIFRLEQFDKCF
                     HDLQIPCRYVPDAGFGYGYPLFNFYAPAFYFLAEIFHLIGFSFTTSVKIILILIKIGG
                     GVGLFLLSRSLLATALFTFAPYQATNLFVRGAFPELLALNLIPWVFYFFKNKNYPWAA
                     FSLALLFLSHSLTSLAVLILLIPYLFFFVKDYKHQLFTFLTAFGLAAFFLLPDFWEKN
                     LT"
     gene            complement(760..2460)
                     /locus_tag="PK131_02235"
     CDS             complement(760..2460)
                     /locus_tag="PK131_02235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPD44970.1"
                     /translation="MLKRNKIILILLIAFFLRTIYLGIYPNNLTNDELHFVLNAKSVF
                     LQFSNLAHNWNPFSLTTIPQESSSELPFVLTSWLIGPLPLDLFFSKLIYVLFGVGTVY
                     LIYCLSQKLFNEKIALLAALIFSLNPWSILVNRTAFDAPLAIFFYLLTLYCLLIYRGK
                     KILWSFIPALIAFYCYIGTKVIFLPIMFCYLIFTFHRNPKDKKYYFIVALLSIILVIF
                     YYSRIGNGASRLNELATPNHPSIINQSLTDRNQSLKTPLKIIFSNKYTVFVENFLVKY
                     FGNFSPNILFLKGDTTYFISLWRHGYFYLIDSIFIILGLIALFNFNRQLFYFIISLIA
                     LSPIPEAIRIDPIPAYAFHSCFQYPFLCVLVALGIDQFLKLKIPLSKLLLIIVYSFSF
                     SYFLYIYFYRYPVYQSEGFFFSRRLVSQYLKLVPNDHPIVVISPEPDSLYRNYLFNSN
                     LLNRNNLKNITEQYQKNSSRELFTEANITFINKIPQTFDKNVTYIIDRQTNWQPKGID
                     FLVINKLNEPANLFFIINDNLCDRNQLDYYVHNLRLADFNLNQLNQQAFCQKYLDRPL
                     "
     gene            complement(2444..>2919)
                     /locus_tag="PK131_02240"
     CDS             complement(2444..>2919)
                     /locus_tag="PK131_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44971.1"
                     /translation="ESNESGDYEFIPLANGKIIEENLKTTHFNLEQISLLFKNPNFKS
                     DQSIFIEINQNSLALTGHSVGDPSWVPFKFPPTNGQINLKIYSPTTPKDLIYLGINQK
                     DKSIATKSVYRIIGFKNRFIANSTFQINRLKNNFTPIIIYGLIILILDCFYVKKK"
BASE COUNT         1100 a          370 c          619 g          830 t
ORIGIN      
        1 ggttaagttt ttttcccaga aatccggcaa tagaaagaaa gcggctaagc caaaggcagt
       61 taaaaaggta aatagctgat gtttatagtc tttaacaaaa aagaaaagat aaggaataag
      121 aagaattaga acggctaaac tggtaaggga atgggagaga aacaggaggg caagactgaa
      181 agcagcccaa gggtaatttt tatttttaaa aaagtaaaaa acccaaggga taagattaag
      241 agctagtaat tcaggaaaag ccccacggac aaataaatta gtagcttgat aaggagcaaa
      301 agtgaacaag gcagtggcta gtaaagatcg agataataaa aataaaccaa ctccccctcc
      361 tattttaatg agaattaaaa ttattttgac gctggtggtg aaagaaaatc cgattaagtg
      421 gaagatctca gctaaaaagt aaaaagccgg ggcgtaaaag ttgaagagag gataaccata
      481 accgaagccg gcatcaggaa cgtaacggca gggaatttgt aagtcatgaa aacatttatc
      541 aaattgctct agtctaaaaa tatgcatttc gtcttgggtt gaatagaaac cggggcgaaa
      601 aagtctcaga caggataaaa gacccaaaat agctgctaga aacactagtt tatttttcat
      661 tctaatttta gttagatagt tatattttac tattaatttc tgcagtttta attttagcag
      721 tcagaactat tgactgctaa tgagagtaag ggcaattaat taaagaggtc ggtcaagata
      781 tttttggcaa aatgcctgtt ggttaagttg atttaaatta aaatcggcta aacgaagatt
      841 gtggacgtag taatcaagtt ggtttcggtc gcataagtta tcattaatga taaaaaataa
      901 gttagctggt tcatttaatt tattaataac gagaaaatca atccccttgg gttgccagtt
      961 ggtttgcctg tcaataatgt atgttacgtt tttatcgaat gtttggggaa ttttgttaat
     1021 aaaggtaatg ttagcctcgg taaaaagttc tctggagcta tttttttggt actgctcagt
     1081 gatgtttttt aaattatttc ggtttaataa attactgttg aataagtaat tgcgatagag
     1141 agaatcaggt tcagggctga taactactat cggatgatca ttgggaacca gctttaaata
     1201 ttgagaaact agtcgtcggc tgaaaaagaa accttctgat tgataaacag gataacgata
     1261 aaaatagata tagagaaaat aagagaaaga gaaactgtaa acgattataa gtaataattt
     1321 cgacagggga attttaagtt ttaaaaattg gtcgatacct aaagcgacta aaacacaaag
     1381 aaagggatat tgaaaacagc tatgaaaagc gtaggctgga ataggatcaa ttctaattgc
     1441 ctccggaata ggagaaaggg cgattaagct aataataaaa taaaacaatt gtctgttaaa
     1501 gttgaaaagg gcaataagac ccagaataat gaaaatggaa tcgattaagt aaaaataacc
     1561 atgccgccat agagaaatga aataagtggt atcaccttta agaaataaga tattgggaga
     1621 aaaattacca aaatatttta ccaggaaatt ctcaacaaaa acggtgtatt tattggaaaa
     1681 aattattttt aagggagttt ttaatgattg gttgcgatca gttaatgact gattgataat
     1741 tgaaggatga ttgggggtgg ccagttcatt taatctagat gcgccattac ctattcgact
     1801 ataatagaaa ataactagaa taatagacag cagggcaacg atgaagtaat attttttatc
     1861 cttaggatta cggtgaaagg taaaaattag gtaacagaac ataatcggta gaaagattac
     1921 tttagtacca atatagcaat agaaagcgat tagggctggg atgaatgacc aaagaatttt
     1981 cttgccccgg tagattaaaa gacaatagag ggttaagagg tagaagaaga tggctaatgg
     2041 ggcatcaaaa gcggttcggt taactaaaat agaccaagga ttaagagaaa aaatcaaagc
     2101 cgctaagaga gctatttttt cattaaataa tttttggctg agacaataaa tcaaataaac
     2161 tgttccaacg ccgaataaaa catagattaa tttagagaag aacaaatcta gaggtagagg
     2221 cccaataagc caagaagtca agacaaacgg cagttcagaa ctactttctt ggggaattgt
     2281 tgtgagacta aagggattcc agttatgagc tagattggaa aattggagaa agacactttt
     2341 ggcattcaag acaaaatgaa gttcatcatt ggttaaatta tttgggtaaa tgccaaggta
     2401 aatagttctg aggaaaaaag cgattaatag aatgaggatg attttatttc tttttaacat
     2461 aaaaacaatc caggatgaga ataattaagc cataaataat aatcggagta aaattgtttt
     2521 tcaggcgatt aatttgaaaa gtagaattag cgataaaacg gtttttgaaa ccgataatcc
     2581 gatagacact tttggtggca atacttttat ctttttgatt aatgcctaag tagattaaat
     2641 ccttcggtgt tgtcggagaa taaattttga gattaatttg gccgttggta ggcgggaatt
     2701 taaatggtac ccagctggga tcaccgaccg aatgaccggt taaagctaaa ctgttctggt
     2761 tgatttcaat aaaaattgac tgatcgcttt taaaatttgg atttttaaac aagagggaaa
     2821 tttgttcaag gttaaaatga gttgttttta agttttcttc gatgatttta ccgttggcca
     2881 aaggaataaa ttcataatca ccactctcgt tgctttcat
//
LOCUS       DAOOIE010000068         2875 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_26852_length_2875_cov_3.476950, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000068 DAOOIE010000000
VERSION     DAOOIE010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26852_length_2875_cov_3.476950"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1504)
                     /locus_tag="PK131_02245"
     CDS             complement(<1..1504)
                     /locus_tag="PK131_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44972.1"
                     /translation="MKKLIQKHCLLIILLFSLPAWVNLLRPVYWNMHDDMQLIRQLEF
                     EKCLNDGQIPCRWTPDLGYGYGYPLFNFYPPLPYLVGQFYRFLGFSFINTVKLTAITQ
                     IILATLAMYYLAKVFFPPLSTILVTLLYTYAPYHAVNIYIRGAMNEAWASVFFPLCFL
                     FSYRLIKTPKTNNLIFLALAYAGLLLSHNPMALTFFPFLLAWIIFWLIIFKKYQKIKI
                     YLNFVYAGLLSLGLTAFFTLPVIFESRLVQVETMFRDYFHYSVHFVSLFQLFISNFWG
                     DGPSVWGTGDQMSFMIGLLHWLIPLLTLVIIIFLAFTKKLEWKKYRLFFLLLLLALGA
                     TFMTHGRSTFIWQSIHVIQKMQFPWRFLNQAAFFFSLSSGFFLLLFKNKKRELVSFFT
                     LLVLIILINAHHFRFVTAGPITDEQKFSGQAWTNQITSGIYDYLPKTAGRAATAPANF
                     PIDMVNPPQSQYELNGVKHGTDWFFFNIKLNYQTELTLAQLAFPNFEIKDN"
     gene            complement(1501..>2875)
                     /locus_tag="PK131_02250"
     CDS             complement(1501..>2875)
                     /locus_tag="PK131_02250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPD44973.1"
                     /translation="SGLYFLSRLINLTKLPIFCDEAIYLRWSQIIKAVETLRFIPLSD
                     GKQPLFMWLTVPFLKLFNDPLMAGRFVSVLAGFGTLVGIYLITCLIANQKTALIATLI
                     YLILPFTFFFDRLALADNLLSMFGVWSLYFSLLITKYPRLDLAMILGITLGLAWLTKS
                     PAIYFIVLAFFTIIQKQAKKFYLPIISVIFALIIYNILRLGPQFHLIALRNRDYVWPV
                     MEILKHPFDPFKPHLVDIFNIYKQYLGLPFFFLFFTQFNPILWAWFILPLLATSIIAK
                     VFTARYILFSLPPLIILLALALGKIKNKLILGGLILTFIPGILFIHRLTIDPFHQKLP
                     PTETGYLSGWTAGWGIKEAADYLKERAKQTNVIVGTEGYFGTLPDGLQIYTNDVKQLT
                     ILGVGLDISQIPPSLTDAFQHGDEVYLLFNQSRLKLSPQEKEKVNLVKSYPKPDNDNL
                     ELYQIIK"
BASE COUNT         1075 a          466 c          626 g          708 t
ORIGIN      
        1 cgttgtcttt tatttcaaaa ttaggaaaag ctaactgagc taaggttaat tcggtttgat
       61 aattgagttt gatattaaag aagaaccaat cggtgccatg tttaactcca ttaagctcat
      121 attgactctg aggaggatta accatgtcaa tggggaagtt ggctggggcc gtggccgctc
      181 gcccagcagt tttgggtaaa taatcgtaaa taccagaggt tatctgatta gtccaggctt
      241 gaccagaaaa cttttgctca tcggtaatcg gtccggccgt aacaaaacga aaatgatgag
      301 cattaattaa aataattaaa actaaaagag taaaaaaaga aaccaattcc cttttcttat
      361 ttttaaatag gagaaggaaa aaacctgaag acagagaaaa gaaaaaagcg gcttgattga
      421 gaaaacgcca tgggaactgc attttttgaa ttacatgaat tgattgccaa ataaacgtcg
      481 atcgaccatg agtcatgaaa gtggctccaa gggcaaggag gagaagaaga aagaataacc
      541 gatatttttt ccattccaat ttttttgtaa aagccaggaa gatgattatt actaaagtaa
      601 gaagaggaat caaccagtga agaagaccaa tcataaaaga catttggtca cctgttcccc
      661 agacagacgg gccatcgccc caaaaattac taataaatag ttgaaaaaga gaaacgaaat
      721 gaaccgagta gtgaaaataa tctcgaaaca tagtttcgac ttgaactagt cgactttcaa
      781 aaataaccgg cagggtaaaa aaggccgtta aaccaagaga caaaagacca gcataaacaa
      841 aattgagata tattttaatc ttttgatatt ttttaaaaat aatcagccag aaaataatcc
      901 aagccaggag aaagggaaaa aaagttaagg ccattggatt gtgacttaaa agcaaaccgg
      961 cgtaagctag ggctaaaaat ataagattat tagttttagg ggttttgata agacggtagg
     1021 agaagagaaa acaaaggggg aaaaaaacac tggcccaagc ttcattcatg gcgccgcgga
     1081 tgtaaatatt gacggcatgg taaggagcat acgtgtataa caaagttact aggatagtac
     1141 tcaatggtgg aaagaaaact ttagccaggt aatacatggc taaagtggct aaaataattt
     1201 gggtaatagc agttaatttg acagtattga tgaagctaaa gccgagaaaa cggtaaaatt
     1261 gaccgacaag atagggtaaa ggggggtaaa aattaaaaag agggtaacca tagccgtagc
     1321 caagatctgg agtccatcta caaggaattt gtccgtcatt taggcatttt tcaaattcaa
     1381 gctggcggat taattgcata tcatcatgca tattccaata caccggccgc aggaggttaa
     1441 cccaagccgg taaagaaaat agaagaataa ttaagagaca atgtttttga atcaattttt
     1501 tcatttgatt atttggtaaa gttctagatt atcgttatct ggcttagggt atgattttac
     1561 cagattaact ttttcttttt cctggggact taattttaat cggctttggt tgaataaaag
     1621 ataaacctcg tcgccatgct gaaaagcgtc agttaaagat ggcggaattt gactgatatc
     1681 taagccaact cctagaatag ttaactgctt gacatcatta gtataaattt gaagaccgtc
     1741 aggcagagtg ccgaaataac cttcagtacc gacaataaca ttggtctgtt tagcccgctc
     1801 ctttaaataa tcagccgctt ctttaatacc ccaaccagct gtccaaccgc tcaaataacc
     1861 ggtttcggtc gggggtaatt tttgatggaa ggggtcaatc gttaaccgat ggataaagag
     1921 aatcccggga ataaaagtta ggattaaacc gcctaaaatg agcttatttt taattttacc
     1981 taaagctaag gctaataaaa taataagcgg cggcagagaa aaaagaatat aacgagcggt
     2041 aaaaacctta gcgataatac tggttgccag aaggggtaga atgaaccaag cccataaaat
     2101 cgggttaaat tgagtaaaga aaagaaagaa aaacggcaga ccgagatatt gtttgtaaat
     2161 attaaaaata tcaaccagat gaggtttaaa cgggtcgaaa ggatgcttga gaatttccat
     2221 aactggccaa acgtaatcgc gattacgcag ggcgatgaga tgaaattgcg gtcccaggcg
     2281 aagaatatta taaatgatca gggcaaaaat taccgaaata atcggcaagt aaaatttttt
     2341 agcctgcttc tgaatgatgg taaaaaaagc caaaacaatg aaataaatag ccggcgattt
     2401 ggtcaaccag gccagaccga gggtaattcc caaaatcatg gctaaatcta gtcgaggata
     2461 cttagttata agaagagaaa aataaagcga ccaaacccca aacatggaca ggagattatc
     2521 ggctaaagcc agtcggtcaa aaaagaaggt aaagggaaga attaaataaa taagggtggc
     2581 tattagagct gttttttggt tggcaatcag gcaggtaatg agataaatgc caaccaaagt
     2641 gccaaaacca gccaaaacag agacaaaacg gccagccatt aatggatcat taaacagttt
     2701 taaaaaagga actgttaacc acataaatag gggttgttta ccatcactta aagggataaa
     2761 tcggagggtt tcaacggctt tgataatctg tgaccaacgg agataaatag cctcatcgca
     2821 aaaaatcggt aatttagtaa gattaatcag tcgggaaaga aaatagaggc cgctg
//
LOCUS       DAOOIE010000069         2838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_27316_length_2838_cov_3.062882, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000069 DAOOIE010000000
VERSION     DAOOIE010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27316_length_2838_cov_3.062882"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..678
                     /locus_tag="PK131_02255"
     CDS             52..678
                     /locus_tag="PK131_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44974.1"
                     /translation="MPWVLDLLFPRKCYDCGKIGQYFCNSCRQQILINSLEPQSNRLS
                     LFKYQKGIKQAILDLKYNFVTDLVDELAELSADSIKGHYSHLWQFWQQKHYLMTAVHL
                     HWWRYNWRSFNQADLLAKNIARRLKLDYRSDLLIRTRHTSPQARLQKRISRLKNVDHA
                     FALNPSVKNQIGNLILFDDVYTTGSTLRSAAEVLKTYCQDIWFLTLAG"
     gene            complement(661..1314)
                     /locus_tag="PK131_02260"
     CDS             complement(661..1314)
                     /locus_tag="PK131_02260"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPD44975.1"
                     /translation="MIILVNFKVYQETWGEGAIKFTSICQRAMEATTTEIIPVVSALD
                     LVRVKAIMGKHKVYLQSLDGDFEGAHNGAITLKQAQILGANGALINHSEKRLKPGTIL
                     QLLKNWPKEMDSVVCLQSWGQVERWAKNIRPTYIAYEPKYLIGNREKSVATEKPEMIK
                     KMVEFYKNIPVLAGAGVRTKEDILVTQKMGGAGVLAAANIIKSDNPEKTLMELASQS"
     gene            complement(1318..2769)
                     /locus_tag="PK131_02265"
     CDS             complement(1318..2769)
                     /locus_tag="PK131_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HPD44976.1"
                     /translation="MNPEEIQSPLPPRRVEIPPVPTTSSSVIKDQTTISQQIQKQQER
                     GLKKYLIWLIAAAIILLIILLLVYFRGRGKTPVSNQIELNYWGLWEESSVMDGIIADF
                     EAKNPNIKVNYKKNSQENYKLRLQNKLAKTETSGETDTPDIFRIHVSWLPLFKNELAP
                     VPTETANRIGLETDFYKVFSKTLKTGSNYKAIPLMYDGLALFYNKDLIGAVGGELPKT
                     WNDLRIIAEKITKRDEQTDKIVTAGAALGSVNNIDHWSDIIGLMLKQGGINPLLNSDD
                     EKIKSVLVYYSLYRTKYHLWDETLPPSTLAFANGSLGFYFGPSWRIFNLRDINPKLNY
                     EVISVPQLENENGTLTNVNWASFWVEGVNTKSKHQKEAFKLLEFLASKEALQKSFTLA
                     SQIRDFGQISPRKSMFDQMGANSKIKPFVDAAETADNWYLSSRTFDESLNDEMTKYFG
                     DAINAIVNQNKDAAEVIITLRSGINQLKTKYGL"
BASE COUNT          801 a          545 c          500 g          992 t
ORIGIN      
        1 gatctatatc ctgttgccct tgcagaggct tagatagcat aaaataaatt tatgccttgg
       61 gtgctcgatc tactttttcc tagaaaatgt tatgattgtg gtaaaattgg tcaatatttt
      121 tgcaactcct gccgtcaaca aattttaatc aattcactcg aacctcaaag taatcgatta
      181 agtttgttta agtatcagaa agggattaag caggcgattc tcgatttaaa atacaatttt
      241 gttactgatt tggttgacga gttagcggag ttatcggctg actccataaa agggcattat
      301 tcccatcttt ggcaattctg gcagcaaaaa cattatctaa tgacggccgt ccatcttcat
      361 tggtggcggt ataattggcg ttcatttaat caggctgatt tattggctaa aaatattgcc
      421 cggcggctta aattggatta tcgatctgat ttattgatta ggacccgtca tacctctccc
      481 caagcccgtc ttcaaaagag aatttctcgg ttgaaaaatg ttgatcacgc ttttgcccta
      541 aatccgtcgg ttaaaaatca aataggcaat ctcattcttt tcgatgatgt ttatacgact
      601 ggttccaccc ttagatcagc cgctgaagtt ttaaaaactt attgccagga catttggttt
      661 ttaactttgg ctggctaatt ccattaaggt tttttcggga ttatcggact taataatatt
      721 ggcggcagct aagactccag cccctcccat tttttgggtt actaaaatat cttctttggt
      781 tctaacgccg gcgccagcta aaacaggaat atttttgtaa aattcaacca ttttttttat
      841 catctctggt ttttctgttg ctaccgattt ttctcgattg ccaattaaat attttggttc
      901 ataagcaata taagttggtc taatgttttt agcccacctt tctacttgcc cccaactttg
      961 gagacaaaca acactatcca tttcttttgg ccagtttttt aataattgta aaattgtccc
     1021 tggttttaat cttttttcac tatgattaat tagagcgcca ttagcaccta atatttgggc
     1081 ctgttttaag gtaatagcgc cattatgagc tccctcgaaa tcaccgtcga ggctttggag
     1141 gtaaaccttg tgtttgccca taattgcttt aactctaact aaatctaaag cagagaccac
     1201 gggaataatt tctgtagtag ttgcctccat agccctttga caaattgaag taaatttgat
     1261 tgccccttcg ccccaagttt cttgataaac cttaaaatta actaaaatga tcatagttta
     1321 tagcccatat tttgtcttta gctgattaat accgcttctt aaagtaataa tgacttcagc
     1381 cgcgtcttta ttttgattga cgatggcatt gatagcgtca ccgaaatact tagtcatttc
     1441 gtcgttgagg ctttcatcaa aggttcggga agaaagatac caattatcgg ctgtctcggc
     1501 ggcgtcaaca aagggtttaa ttttagagtt ggcccccatt tgatcaaaca tactttttct
     1561 tggagaaatt tgaccaaaat ctctaatttg actagccaag gtaaaacttt tttgcaaagc
     1621 ttctttagag gctaaaaatt ctaacaattt aaaggcttct ttttggtgtt tacttttcgt
     1681 attaactcct tctacccaaa aactagccca attgacgtta gttaaggtac cattttcatt
     1741 ttccaattga ggaacagata taacttcgta gtttaatttg ggattaatat cccttagatt
     1801 aaatatccgc cacgagggac caaaataaaa gcccaaactg ccgttagcaa aagccaaagt
     1861 agacggaggt aaagtttcat cccagagatg atatttagtt cggtaaagac tgtagtaaac
     1921 taaaaccgac ttaatttttt catcgtcact attaagaagg ggattaatcc ctccttgttt
     1981 tagcattagg ccaataatat cactccaatg atcgatatta ttgactgaac ctaaggcagc
     2041 cccagcggta acaattttgt ctgtttgttc atctcttttg gtaatttttt ctgctattat
     2101 ccgaaggtca ttccaggttt ttggtaattc acccccaacc gccccaatta aatctttatt
     2161 ataaaaaagc gctaaaccgt cgtacattag aggaatggct ttataattag accctgtttt
     2221 taaagttttt gagaaaactt tataaaaatc ggtttctaat ccaatacgat tggcagtttc
     2281 ggtggggact ggagccaatt catttttaaa caaaggcagc caagagacat gaattcgaaa
     2341 aatatctgga gtatcagttt ccccgctggt ttctgtttta gctaatttat tttgcagtct
     2401 aagtttataa ttttcctgac tattcttttt gtaatttacc ttgatattgg gatttttagc
     2461 ttcaaaatca gcaataattc catccataac actgctttct tcccataatc cccaataatt
     2521 taactctatt tgattgctta ccggggtttt cccccgacct cggaagtaaa ctaataaaag
     2581 aataattaaa agaataatcg ctgctgctat cagccaaatt agatattttt ttagtcccct
     2641 ttcctgttgt ttttgaattt gctgactaat ggtggtctga tccttaataa cggatgaaga
     2701 ggttgttggc actgggggaa tttcgactct tcttggcggt agaggtgatt ggatttcttc
     2761 tggattcaca gttatatgat aactaatttt tttgataaac tccattatgt atggttgtga
     2821 ctatttatac tgatggcg
//
LOCUS       DAOOIE010000070         2828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_27459_length_2828_cov_2.443563, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000070 DAOOIE010000000
VERSION     DAOOIE010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27459_length_2828_cov_2.443563"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PK131_02270"
     CDS             <1..196
                     /locus_tag="PK131_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44977.1"
                     /translation="IVMILPVIRYKNEFFYLDILDKVKFKQQKYCDYQEKLGLNLTNR
                     GTIVYYRPGQTVSREIIIWK"
     gene            187..675
                     /locus_tag="PK131_02275"
     CDS             187..675
                     /locus_tag="PK131_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44978.1"
                     /translation="MEIKEAKSESAVNHRLIVTYNDYIHQYLKQSEAEIVTFADGVQK
                     IKILKGGNLPEVKSLTEFQKEIEDQNGLIIKNADDLVKDLMSRLTKIESDDQLAALIN
                     SAYRLFDKEAIGESYWRNRNKNKWPETITVKTMPNQLDQWIEKSRIITLPVESLFPSS
                     SK"
     gene            complement(632..1849)
                     /locus_tag="PK131_02280"
     CDS             complement(632..1849)
                     /locus_tag="PK131_02280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD44979.1"
                     /translation="MKPPTETAVIIIPTYNEAKNIGKMIVYLNEKIFPSLDKKWQMKI
                     LVVDGNSPDGTGKVVQNYAKKYKNTYLYTETSKDGIGAAYLKGFKYALDNLGADYVFE
                     FDGDFQHPPEAIPVMLKAMDEGYDYVIGSRKIKGGSNPKGWGLKRVFYSDVGGWIARF
                     ILFFPFKNFWRVTDPTTGLKVTRVKGFIDRMDLDWQGDRLLTKSFGYKLQLLYETLQM
                     GAKFKEIPLQFQVRNAGESKIESRTAKEIFRVVLLLRWRDEFTQKFVKFALVGGLGFV
                     INVVGAKIFKNLLIKPGVNLSLLNGICNALAAEISIISNFIWNNVWTFAREKITSFKL
                     LLSKFITFNLSSILTGIIIPSIGVSLLTALFGDHLFLYQVIVIFGLTVPLNWLVYNRV
                     IWKKKEKVIRPAK"
     gene            1866..2513
                     /gene="trmD"
                     /locus_tag="PK131_02285"
     CDS             1866..2513
                     /gene="trmD"
                     /locus_tag="PK131_02285"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010417579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPD44980.1"
                     /translation="MRIDLVTLFPSMFTSPFAESIIQRAIKKKLIDIKIHNLRDWAVD
                     KYKTVDDKPFGGGVGMILKVDVIDRCLKDIGPGHTILLSPRGKRFTEQKAETLAKLDR
                     IILICGHYEGVDQRVADHLVDEEISIGDFITTGGELPAMLIIDSVVRLLPGVLGKNES
                     SQEESFSSKLNRKIEYPQYTRPAEYRGWSVPTALLSGNPKLIEEFKKKNTQEKTD"
     gene            complement(2445..2759)
                     /locus_tag="PK131_02290"
     CDS             complement(2445..2759)
                     /locus_tag="PK131_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012342644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPD44981.1"
                     /translation="MGVIFYFSSKSTAAVPLTGGWRFLFFKSLHLIEYAILAICLGWA
                     LIKRKNIIIIGYLYALSDEIHQFFVPGRESRFQDTLIDLIGLFLGIFLFKFLNQFWIA
                     RK"
BASE COUNT         1011 a          545 c          504 g          768 t
ORIGIN      
        1 tatagtaatg attttaccag taataaggta taaaaatgaa tttttctact tggatatttt
       61 agataaagtt aaatttaaac aacaaaaata ttgtgattac caagaaaaac ttggcttaaa
      121 tctgaccaat agaggtacta ttgtttatta caggccagga caaaccgttt cccgggagat
      181 tattatatgg aaataaagga agccaaaagt gaatcggctg tcaatcatcg acttatcgtc
      241 acttataacg actatatcca ccaatatctt aaacagtccg aagcagaaat agttaccttc
      301 gctgacggag ttcaaaaaat aaaaatttta aaagggggta atttgccgga ggtaaaatca
      361 ttaactgaat ttcaaaagga aatagaagac caaaatggtc tgattattaa aaatgccgac
      421 gacttagtaa aagatttaat gagtcgactg actaaaatag agtccgacga tcaactggcc
      481 gctttaatca acagcgctta taggctattc gacaaagagg ctattgggga aagttactgg
      541 agaaatagga ataaaaataa atggccggaa acaattacag tcaaaactat gcctaatcag
      601 cttgatcaat ggatcgaaaa aagtcggatt attactttgc cggtcgaatc actttttcct
      661 tcttcttcca aataacccga ttataaacca gccaatttaa gggaacagtt aagccaaaaa
      721 taacaataac ctgataaagg aaaagatgat cgccgaaaag agctgttaac agagaaacac
      781 caattgaggg aataataata cccgttagaa tcgaggaaag attaaaggta ataaatttac
      841 tcaataagag tttaaaacta gttattttct cccgggcgaa ggtccagaca ttattccaaa
      901 taaaattaga aataattgaa atttcagccg ctaaagcatt gcatattcca tttaaaagac
      961 ttaaattaac gccgggttta attaaaagat ttttaaaaat tttagcgccg acgacattaa
     1021 tcacaaaacc taaaccacct actaaagcaa atttaacgaa tttttgggta aattcatccc
     1081 gccagcgaag gagaagaact actcggaaaa tttctttagc agttctagat tctatttttg
     1141 actcgccggc atttcttacc tggaactgca agggaatttc cttaaattta gcccccattt
     1201 gcagggtttc ataaagcagt tgaagtttat aaccaaaact tttagttaaa agccgatcac
     1261 cctgccaatc taaatccatt cgatcgataa aaccctttac tcgagtaact tttaaaccgg
     1321 tagttgggtc agttactcgc caaaaatttt taaaaggaaa aaagagaata aaacgagcga
     1381 tccagccgcc aacatcagag taaaaaaccc gctttaaacc ccaaccttta ggattggaac
     1441 cacctttaat ttttctcgag cctataacat aatcgtagcc ttcatccatc gctttcaaca
     1501 tcaccggaat tgcctccggc ggatgctgaa aatcaccgtc aaactcaaaa acataatcag
     1561 cccccaaatt gtcgagagca tacttaaaac ccttcaaata agcagcaccg atgccatctt
     1621 tagaagtttc agtataaaga taggtatttt tatatttttt agcataattt tggacaactt
     1681 tacccgtccc atcaggcgaa ttaccatcaa cgaccaaaat tttcatctgc cactttttgt
     1741 ccaaagatgg gaaaattttc tcatttaaat agacaatcat ttttccaata tttttagctt
     1801 cgttataagt tggaataata ataaccgccg tttccgttgg tggcttcata tgagataatc
     1861 ataccatgag aattgatctt gtcaccttgt ttccctcaat gtttacctcg cctttcgccg
     1921 aaagcataat ccaacgggca ataaagaaaa agctcatcga cataaaaatt cataacctgc
     1981 gggattgggc tgttgataaa tataaaacag tcgacgataa accctttggt ggtggggtcg
     2041 gcatgatttt gaaggtagat gttattgatc ggtgtttaaa agatatcggt ccgggacaca
     2101 ctattttgtt gtctccgcgg ggaaaacgat ttaccgaaca aaaagctgaa acactggcaa
     2161 aattagatag aattattcta atttgcggcc attatgaggg ggttgaccaa cgagtcgccg
     2221 atcatttagt cgatgaggaa atttccatcg gcgatttcat cactaccggc ggtgaactgc
     2281 cggctatgct tattattgac agcgttgtta gattattacc cggagttttg ggtaaaaacg
     2341 aatctagcca agaagaaagt ttttcatcta aactaaaccg gaagatagaa tatcctcaat
     2401 atacccggcc agccgagtat cggggttggt cagtcccaac agccttactt tcgggcaatc
     2461 caaaactgat tgaggaattt aaaaagaaaa atacccagga aaagaccgat tagatcgatt
     2521 aaagtatctt gaaaccggct ttctctcccg ggaacaaaaa actgatggat ttcatctgat
     2581 aaggcgtata aatagccaat tattataata ttcttccttt tgatcagcgc ccagcctaaa
     2641 caaatagcca aaatagcgta ctcaattaaa tgaagcgatt taaaaaacaa aaaccgccag
     2701 ccgccagtta aaggaacagc ggcggtactt tttgatgaaa aataaaaaat aactcccatc
     2761 caaatcaaac ttggccaaaa gcgaaaaaac ttttgatgaa ctgaaagaaa tagccgtgaa
     2821 agagaaat
//
LOCUS       DAOOIE010000071         2762 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_28396_length_2762_cov_2.701145, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000071 DAOOIE010000000
VERSION     DAOOIE010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28396_length_2762_cov_2.701145"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="PK131_02295"
     CDS             complement(<1..416)
                     /locus_tag="PK131_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44982.1"
                     /translation="MSLTTTAFKTRQAIKIGLAVIIFIPILWGGLSYGLKKYRAAKPI
                     QAEPNIKYGILPKIQFPEKKFEAKNFSLELVSDQLPNFGNQARVYVVFRTDKDFMALE
                     TEIQTAKQLGFNDQPRLVGPNIYEFQNNYNQKLVIN"
     gene            449..1768
                     /gene="gltX"
                     /locus_tag="PK131_02300"
     CDS             449..1768
                     /gene="gltX"
                     /locus_tag="PK131_02300"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPD44983.1"
                     /translation="MVRTRFAPSPTGEIHLGALRTLLYNYALAKHYQGRFILRIEDTD
                     RKRYVEGAEKRIIEDIKAYGLSWDEGPFYQSQRAAIYQQYAQQLLAHGQAYYCFCSPE
                     RLAGLKTKYDRYCLRLSQREIKEKLEKGEPYVIRLKMPDGEVITFNDAVRGEISFQTD
                     DLDDQVLIKSDGLPTYHLAVVVDDHLMEISHVLRGDEWISSTPKQILLYRYFGWKPPI
                     FAHLTALLDASGKGKMSKRSGATMARQFLEEGFLPEAVLNYLMLLGWNPGTDQEFFSL
                     NEFVKVFSLEHLHKSQPAFDRQKLLYFNGFYLRQKTDKELARLLPQADPRFIPLIKSR
                     IFTLNDADNLLEFATKDLIPPRELLDLEMLKEIKNILETKDFANLQNEFLNLIKEKKY
                     QTGDFFMTLRLAICGKKITPPIVESMLIIGREACLRKINKVLENKSQ"
     gene            1859..>2762
                     /locus_tag="PK131_02305"
     CDS             1859..>2762
                     /locus_tag="PK131_02305"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPD44984.1"
                     /translation="MLFPALLIVILLFTSVNSALAQVKISDFCADCDPEWVDLINVGT
                     SSVSLDQWNLLNRNGYKLPLSGIINSNEVLRFIKTKGWLNNEEGKIILINGIGETIDQ
                     IAYPTPTPIPIPTNTPIPSPLSTATPTPIPTPILTPTTILVPTNTLIPTLTPTPLPPP
                     VDHLYLNEIMPYPETGSEWVEIFNDNDFEINLENYKIKDNSSRYRLIVGDNPVLAHSY
                     FVFLFANYLNNDGDSLSLFDPFNRQIGESFSYTTAKKGLSYSWQENNSWCLSFSSMNQ
                     DNNSCYTEPTAVPTPTHTPKPTATP"
BASE COUNT          826 a          491 c          532 g          913 t
ORIGIN      
        1 atattgataa ctaatttttg gttatagttg ttttgaaatt cataaatatt agggccaacc
       61 aatcttggct gatcattgaa gcctagttgt ttggcagttt gaatttctgt ttccaatgcc
      121 ataaaatctt tgtcagttct aaagacaaca tagactctgg cctgattgcc aaagttaggc
      181 aattgatcgc ttactaattc taaagaaaag tttttagcct caaatttttt ctccggaaat
      241 tgaattttag gtaggattcc atatttaata ttaggttccg cctgaatcgg cttggcggct
      301 cggtattttt taaggccata tgataatcct ccccataaga ttggaataaa aataataact
      361 gccaagccta ttttaattgc ctgcctggtt ttaaaagcag tggtagtcaa ggacataatt
      421 aattttacct taaatgttaa aataaaacat ggttcgaact cgttttgctc ctagtcctac
      481 tggtgagatt catttggggg ccttgcgcac tcttctttat aattatgctt tagctaaaca
      541 ttatcaaggc cgatttattc tccggattga agataccgat cgaaaaagat atgtcgaagg
      601 ggcagagaaa agaattatag aagatattaa agcttatggt ttgtcttggg atgaaggccc
      661 tttttatcag agtcagcgag cggctattta ccagcagtat gctcagcaat tattagctca
      721 tggccaagct tattattgtt tttgttctcc cgagagatta gctgggctca aaactaaata
      781 cgatcgttac tgtctccgat taagccagag agagattaaa gaaaaattag aaaagggcga
      841 accttatgta attcgtctaa aaatgcctga tggagaggtt attaccttta acgatgctgt
      901 tagaggtgaa atttcctttc aaactgatga tttagatgat caggttttaa ttaagtctga
      961 cggtttacca acttatcatt tggcagtagt agtcgatgat catttgatgg agattagtca
     1021 tgttttaaga ggtgatgaat ggatttcatc taccccgaaa caaattctgc tttatcggta
     1081 ttttggttgg aagccgccga tatttgccca tttaacggct cttttagatg cctccggtaa
     1141 agggaaaatg agtaagcgaa gtggagcaac aatggctagg caatttttag aagagggttt
     1201 tttgccggaa gcagttttga attatttaat gcttttaggt tggaatccag ggactgacca
     1261 ggaatttttt tctcttaatg agtttgttaa agttttttcc ttagaacatc ttcataaaag
     1321 ccagccagcc ttcgatcgac aaaaattgct ttatttcaat ggattttatc tgcggcaaaa
     1381 aactgataaa gaattggctc gacttttacc ccaggctgat ccgcgattta ttcccttaat
     1441 taaatcccgt atttttaccc taaatgatgc ggataatttg ttggaatttg caactaagga
     1501 tttgattccg cctcgggagt tgcttgattt ggaaatgtta aaggagatta aaaatatttt
     1561 agaaactaaa gattttgcca atctccaaaa tgagttttta aatttaatta aagaaaaaaa
     1621 atatcaaacc ggcgattttt ttatgacttt aaggctggct atttgcggca aaaaaattac
     1681 tccgcctatt gttgaaagta tgttgattat cggtcgagaa gcttgtttgc ggaagataaa
     1741 taaagttcta gagaacaaga gccaataatc agtccaattt ctaagaaaat aacagtcaag
     1801 agcttgggtt aaaaagtgtc taattgtgta tttctccgcg tcttgctaat cttagtcaat
     1861 gctttttcct gctctgctga ttgttatttt gcttttcact agtgtaaatt cagctttagc
     1921 tcaggtaaaa attagtgatt tttgtgctga ttgtgaccct gaatgggtag atttgataaa
     1981 tgttggtacc agttctgtgt cccttgatca atggaattta ttaaatagaa atggatataa
     2041 attacccctg tcgggcataa ttaattcaaa tgaggtatta agatttataa aaaccaaagg
     2101 ttggttaaat aatgaagaag ggaaaataat tttgattaac ggtattggtg agactattga
     2161 tcaaattgct tatccaacgc ctactcccat tcctattcca accaacactc ctatcccttc
     2221 tcccttgtcg acagcgactc ctactcctat tcccacacca atactgacac cgacgacaat
     2281 cttggttcca accaacacct taatcccgac tttgacgccg acgcctctgc ctcctccagt
     2341 cgatcacctt tatcttaatg aaattatgcc ttatcccgaa accggcagtg aatgggtaga
     2401 aatcttcaac gataatgatt tcgagattaa tttggaaaat tataaaatta aagataacag
     2461 tagtcgttac cggttaattg tcggtgataa tcctgtttta gctcacagtt attttgtttt
     2521 tctatttgcc aattatttaa ataatgatgg tgattcgctc tctctttttg atccgtttaa
     2581 tcggcagatt ggcgagagtt tttcctatac tacggctaaa aaaggtttat catacagctg
     2641 gcaggaaaat aacagctggt gtttaagttt cagttcgatg aatcaggata ataacagctg
     2701 ttataccgag ccgacagcag tgccgacgcc aacccatact cccaagccga cagctactcc
     2761 ca
//
LOCUS       DAOOIE010000072         2761 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_28410_length_2761_cov_2.882483, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000072 DAOOIE010000000
VERSION     DAOOIE010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28410_length_2761_cov_2.882483"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="PK131_02310"
     CDS             complement(<1..312)
                     /locus_tag="PK131_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44985.1"
                     /translation="MGSKIYSKNFSAIGNLSSDQQTIQIQEDFFLSELGFSKMENNSS
                     SDIYLEVFVNERSLFDPSLLHLGTLKRNNGNGYAFPDAVKIGAKETVKLSLKNNTGAS
                     LS"
     gene            complement(355..972)
                     /locus_tag="PK131_02315"
     CDS             complement(355..972)
                     /locus_tag="PK131_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44986.1"
                     /translation="MIRRDQKLYDSVQIHTGIAANTEAEFFQQPLGSSPSQITTGTVA
                     TYQKSDYHSNNVLNGQLRKGQSMVVRGINIFADITDVNVLKQVLIDSKAVFQFWRAEE
                     KVFQAPVYEMVDIKAVSISGFADASSTTKAQKFSVAFKGEPFMFKKWGITIEELQTLK
                     AKIIFKSAVTFKSFTFDYGSAAANAAYTTEFNLFVSLDVEETLAG"
     gene            complement(991..1662)
                     /locus_tag="PK131_02320"
     CDS             complement(991..1662)
                     /locus_tag="PK131_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44987.1"
                     /translation="MAKKRKAKKKAVAKAAVIRRLSPAKKSYRRKNPSGGKSGLMGIL
                     MEAGAAVLGYVASDYAVELLGMKYAPKKQEGLTVVKKSGEKDVWEVTSKRGSETLTTS
                     EAIGKGYLVKGLIPNVLIPGALGALVYSYLGKGKNGNLMKAGALGMMANAGVEAVESF
                     TKATSGKNIVSTLQGLGQGYEAIPSGVDLNYIQQLAMESARAAAAENAVVNGLGNGYF
                     DLTKK"
     gene            complement(1739..2146)
                     /locus_tag="PK131_02325"
     CDS             complement(1739..2146)
                     /locus_tag="PK131_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44988.1"
                     /translation="MGLQEYKAEEIDRLKEQITELRISQNNFKERLVELSSQNNNHFD
                     RLFREIEKVQKTMEGINHLTLQITMQVKSLNDHDKKIALMEREIKDTIVFDTKTREMI
                     KEHAIQERTALKVVGVVFAVVQTAVIFLMKYLQ"
     gene            complement(2146..2553)
                     /locus_tag="PK131_02330"
     CDS             complement(2146..2553)
                     /locus_tag="PK131_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44989.1"
                     /translation="MSFIFGVLVLGLVAGLMWFLARFFKFDQSVIMDSEYEREIQRIR
                     EKIHAGTPEQKRKNRDWMKGLMILAVLFIFSCSGKKLVVLPEPPAFPAFSFVEVENGF
                     LFSDEEIARFRVYMESQKLYREKLLETLERLEK"
     gene            complement(2550..>2761)
                     /locus_tag="PK131_02335"
     CDS             complement(2550..>2761)
                     /locus_tag="PK131_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44990.1"
                     /translation="FMSFWASLKGRFQDLILECVSYKTLLLVLALVLAGRADFSSLDH
                     LSRFVAEMSAVAGVGVYKIIKDKRQ"
BASE COUNT          763 a          660 c          499 g          839 t
ORIGIN      
        1 tgaaagagaa gccccggtat tgttttttaa agacagtttc actgtttctt ttgctccgat
       61 tttaacggca tcaggaaaag catatccgtt tccgttgttt cgtttcagcg ttcccaaatg
      121 aagcagcgac ggatcaaata acgaccgctc attcacaaag acttccaaat aaatatccga
      181 actggaattg ttttccattt ttgaaaaccc taactcagat aagaaaaaat cttcctgaat
      241 ctggattgtc tgctgatctg aagaaagatt accaatggct gaaaagtttt ttgaataaat
      301 tttgcttccc attttttttc ctttttaagg gggcggtaaa gcccccgttt ttttttaacc
      361 ggctaaggtt tcttctacat ccagagaaac aaacagatta aattccgtag tgtaagcggc
      421 attggcggcg gcagatccat agtcaaaagt aaaactcttg aaagtcacgg cacttttgaa
      481 aatgatttta gccttcaggg tctgaagctc ttcaatggta atgccccact ttttgaacat
      541 aaaaggctcg cctttaaaag ccacagaaaa cttttgggct tttgtggtag acgaggcgtc
      601 ggcgaaaccg gaaatagaaa cggccttaat atctaccatt tcatacacag gggcctgaaa
      661 aactttttct tcggctctcc agaactgaaa aacggctttc gagtcaatca gaacctgctt
      721 cagaacattg acatcggtaa tatctgcgaa gatattaatt ccccgaacta ccatgctctg
      781 gcctttgcga agctgaccat tcagcacatt gttagaatgg taatcgcttt tctgataagt
      841 agccacggtt ccggtggtaa tctgagaggg tgaagaacct aaaggctgct gaaagaattc
      901 cgcttcagta ttagcggcga ttccggtgtg aatctgcacc gaatcgtaaa gtttttgatc
      961 tcgtctgatc ataagacatt ttctcctttt ttatttttta gtgaggtcaa aataaccgtt
     1021 tccaaggccg tttaccacgg cattttcagc agcggcggct ctggctgatt ccatggccaa
     1081 ctgctgaatg taatttaaat caacaccgga agggatcgct tcataaccct gaccaaggcc
     1141 ttgaagggta gaaacaatat tttttccaga agtagctttt gtaaacgact ccaccgcttc
     1201 cactccggcg ttagccatca ttcctaaagc gccagccttc attagatttc cgtttttacc
     1261 ttttccaagg taagaataaa ccagcgcacc taaagcccct ggaatcagca cattaggaat
     1321 taaacccttg accagataac cttttccgat ggcttcagaa gtggttaaag tttcacttcc
     1381 tcttttggaa gtgacttccc agacatcttt ttcaccggat tttttaacca cagtaagtcc
     1441 ttcctgtttc ttaggggcat atttcatgcc cagcagttct acagcataat ccgatgccac
     1501 ataacctaaa accgccgctc cggcttccat taaaatcccc atgaggccgg atttaccgcc
     1561 ggaaggattt ttgcgccgat agcttttttt tgccggactt aaccggcgga tcactgccgc
     1621 ttttgccacg gctttctttt tggcttttct ttttttagcc actttaatct cctttacttt
     1681 tttttatgta aaggctgccg ggaaggtctt ttttttcaaa ggccttcccg aaaacccttt
     1741 actgcaaata tttcatcaaa aaaattacag cggtttgaac caccgcaaaa acaacgccaa
     1801 cgacttttaa tgccgtgcgt tcctgaatag catgttcttt tatcatttcc ctggtcttag
     1861 tatcaaaaac aatagtgtcc ttaatctccc gttccattaa ggctattttt ttatcatggt
     1921 catttaaact ttttacttgc atggtaattt gaagggttaa atggtttatt ccctccattg
     1981 ttttctgaac tttttcaatt tccctgaaaa gccggtcaaa atgattatta ttctggcttg
     2041 aaagttctac aagcctttcc ttgaaattat tttgactaat tcttaattct gtgatctgtt
     2101 cttttaagcg gtcgatttct tccgctttgt attcctgtaa gcccattatt tttccagcct
     2161 ttcaagggtt tctaacaatt tttcacgata cagcttctgg ctttccatat acacccgaaa
     2221 ccgggcaatt tcttcatcag aaaaaagaaa gccgttttct acctctacaa acgaaaaggc
     2281 cggaaaggcc ggtggctcag gaagaaccac cagttttttc ccggaacagg aaaagataaa
     2341 aagcaccgca agaatcatca ggcctttcat ccaatcccga tttttccgtt tctgctccgg
     2401 cgttccggcg tgaatctttt cacggattct ttgaatttct ctttcatatt cagaatccat
     2461 aatcaccgat tgatcgaatt taaaaaaccg ggctaaaaac cacatcagcc ctgcaactaa
     2521 acccaaaacc aaaacgccga aaataaaact cattgccgtt tatcctttat gattttatac
     2581 actcccaccc ccgccaccgc cgacatttca gccacaaagc gggaaagatg atctaagctt
     2641 gaaaaatctg cacggcctgc cagcaccaaa gccagcacca gcaaaagggt tttatagctc
     2701 acacactcta aaatcaggtc ttgaaaccgg cctttcaggc ttgcccagaa actcataaaa
     2761 c
//
LOCUS       DAOOIE010000073         2747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_28633_length_2747_cov_3.333952, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000073 DAOOIE010000000
VERSION     DAOOIE010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28633_length_2747_cov_3.333952"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="PK131_02340"
     CDS             <1..875
                     /locus_tag="PK131_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UbiA prenyltransferase family protein"
                     /protein_id="HPD44991.1"
                     /translation="QWIKNLSIFAASILNGQFLNPFIFTKSLLGFIAFCLLSSSSYLI
                     NDLVDIEKDKLHPFKKERPIARGDVTPGEALTVSLLFLIGGLLIARGVNQAFFIVCFL
                     FIILQYSYSFFLKKKAIIDIVGIALFFIIRTYAGEVASGYHLPVWIMLTVIFLALFIA
                     AGKRYSEICGSGSKTRWSLEGYGKNLLDFYITMFGVCTLICYALFTFFAEPISFNGTL
                     HFYLQNHFPQLIDRKWYMVTLLPVIFGLMRYGQIIFEMQEGERPERVVATDIPLLLAI
                     IVWGLMMITIVYVF"
     gene            865..2367
                     /locus_tag="PK131_02345"
     CDS             865..2367
                     /locus_tag="PK131_02345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPD44992.1"
                     /translation="MFFNKKKLTIGFVIGCLLFSRFVKINWGDGFFFHPDENNMARAI
                     TNLSNTNLDPHFYAYGQFPLYLVYFIFRGVTLKSPSFAQAVLGLRLTSAVFSCLTIYC
                     FYLLSKEVFRCFNDQIIFLLFLIFSPGLIQIAHFGTTESILVFVFVFDIYLAFLLKRK
                     YQFKYLIWAGIITGVGVATKITALVFTLPIIIALKFSLLPTVYYLLITLMTGIGLSPY
                     SLINFPDFLSSMRYETGVARGATKVFYTNQFLSTKPYWFQIKNIFPYVVGLPVFIFSC
                     LGLIKIRHHRNFKTIFSFFLLPSLIYFLYFGQLYVKWTRFMAPIFVVFPLLAAYWISQ
                     LKSKSFKSIMIILAILPGIIFLNLYLRPDIRLSASQWMDENLNDGSVILSEAGNIVDL
                     PLNTSRSFMVRNFDFYNLENSPDKLADLYQYVERANYILVPSRRVFKNQTGSRFPSSS
                     QYYQDLFSGQLGFSLVKTFQTPNYFILDPENAEETWTVFDQPKIRLYARR"
     gene            2364..>2747
                     /locus_tag="PK131_02350"
     CDS             2364..>2747
                     /locus_tag="PK131_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD44993.1"
                     /translation="MKKYYIYIILIIAFLLRIVGLNWDDYGHLHPDERFLTMVETSIQ
                     LPKSVREYFDTQISPLNPYNKGNSFFVYGTFPVFLTKIIGQLTGLTGYNQIHFVGRVL
                     SALFDTLIVYFLYLLTKKKFLLPSLI"
BASE COUNT          709 a          457 c          458 g         1123 t
ORIGIN      
        1 ctcaatggat aaaaaattta agcatttttg ccgcttcaat tttaaatggc caatttctta
       61 atccatttat ttttactaaa tcattattgg gttttattgc cttttgttta ttgtcatcct
      121 cctcttattt aattaatgat ttagttgata tagagaaaga taagctccat ccttttaaaa
      181 aggaaagacc aatcgctcgg ggagatgtca cccctggtga ggctctaacg gtttcattat
      241 tatttttaat tggaggttta ttaattgccc gtggagttaa ccaagccttt tttattgttt
      301 gttttctttt tattatcctt cagtatagtt attcattttt cttaaagaaa aaggccatta
      361 tcgatattgt cggtattgct ctctttttta ttattcgaac ttatgctggc gaagttgctt
      421 ccggctatca tttaccggtt tggattatgc tgacagttat ttttctggct ttgtttatag
      481 ctgccggtaa acgttattct gaaatttgcg gctctggctc taaaacccgc tggtctctgg
      541 aaggttacgg taaaaattta ctcgattttt atattacaat gtttggtgtt tgtaccctga
      601 tttgttatgc tctttttact ttttttgccg aaccgattag ttttaacgga actctccatt
      661 tttatctcca aaatcatttc ccccaattga ttgatcgtaa atggtatatg gttactttac
      721 tgccggttat ttttggtcta atgcgttatg gacaaattat ttttgagatg caggaaggtg
      781 aacgtccgga aagagtagtt gctaccgata tccccttact tttggcaatt attgtctggg
      841 gcttgatgat gattactatt gtttatgttt tttaataaaa aaaaattaac aataggattt
      901 gttattggtt gtcttttatt cagccgtttc gttaagataa actggggcga tggttttttc
      961 tttcatcctg atgaaaataa catggcgcgg gccataacta atttgtctaa cactaatctc
     1021 gacccccatt tttatgctta tggtcaattt cctctttatt tggtttattt tatttttaga
     1081 ggagtaactt taaaatcacc ttcatttgct caagctgttt taggtttgag actgacctca
     1141 gcagtttttt cttgcctaac tatttattgt ttttatctgc tttctaagga agtttttcgg
     1201 tgttttaatg atcaaataat ttttttactt tttttgattt ttagcccggg actaattcag
     1261 atagcccatt ttggtacaac cgaatcgatt ttagtttttg tttttgtctt cgatatttat
     1321 ttggcttttc ttttaaaaag aaaatatcaa tttaaatatc ttatttgggc ggggataatt
     1381 accggtgtag gagtagcgac taagattacc gccttggttt ttactttacc tataatcata
     1441 gcccttaaat ttagtttatt gccaactgtt tattatttat taatcacttt aatgactggt
     1501 attggtttat ctccttactc attaatcaat ttccctgatt ttctttcttc gatgcgttat
     1561 gaaactggtg tagctcgggg agcaaccaag gttttttata ccaatcaatt tttatcaact
     1621 aaaccatatt ggtttcagat aaaaaatatt tttccttatg tggttggttt acctgttttt
     1681 atcttttcct gtttaggttt aattaagata agacatcata gaaatttcaa aacaattttt
     1741 tccttttttt tattgcctag tttaatttat tttctttatt ttggtcagct ttatgtaaaa
     1801 tggacccggt tcatggcgcc tatttttgtt gttttcccgc ttttagcggc ttattggata
     1861 agccaattaa aatcaaaatc tttcaaatca attatgatca ttttggctat tttacccggt
     1921 attatttttt tgaatttata tttaaggccc gatattcgtc tgtctgcctc tcagtggatg
     1981 gatgaaaatt tgaatgatgg atcggtaatt ttatccgaag ctggcaatat agttgatctg
     2041 cctctgaata ccagccgctc ttttatggtc agaaatttcg atttttataa tttggaaaat
     2101 tcaccagata aattagccga tctttatcaa tatgtggaac gggcaaatta cattttagtt
     2161 ccttcccgtc gggtttttaa aaatcaaact ggctcccgat ttccatctag tagccagtat
     2221 taccaagatc ttttttccgg ccaactaggt tttagtttgg tcaagacgtt tcagacccct
     2281 aattacttta ttctcgatcc tgaaaatgct gaggaaacct ggaccgtttt tgatcagccc
     2341 aaaataaggt tatatgccag gcgatgaaga aatattatat ttatattatt ttaataattg
     2401 cctttttatt aagaatcgtt ggtttgaatt gggatgatta cggtcatctt catccggatg
     2461 agagatttct cactatggta gagacctcta ttcagttgcc taaaagtgtt agagaatatt
     2521 ttgataccca aatatctccg ttgaatccgt acaataaggg taatagtttt tttgtttatg
     2581 gaacttttcc tgttttttta actaagataa ttggacaatt aactggctta accggttata
     2641 atcaaatcca ttttgttggc cgcgtcctat cagccctttt tgacaccttg attgtttatt
     2701 ttctttatct acttactaag aaaaaattcc tcttaccttc actaata
//
LOCUS       DAOOIE010000074         2734 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_28834_length_2734_cov_3.119821, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000074 DAOOIE010000000
VERSION     DAOOIE010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28834_length_2734_cov_3.119821"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..112)
                     /locus_tag="PK131_02355"
                     /pseudo
     CDS             complement(1..112)
                     /locus_tag="PK131_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433196.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
     gene            complement(143..382)
                     /gene="rpmE"
                     /locus_tag="PK131_02360"
     CDS             complement(143..382)
                     /gene="rpmE"
                     /locus_tag="PK131_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013756860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPD44994.1"
                     /translation="MKQGIHPKYYHQAKVNCSCGYQFTTGSTQETIEVEVCSHCHPFY
                     TGQQKFVDVRGRIDKFNEKVAKGKSYTKKTKKGKK"
     gene            complement(498..1706)
                     /gene="hisS"
                     /locus_tag="PK131_02365"
     CDS             complement(498..1706)
                     /gene="hisS"
                     /locus_tag="PK131_02365"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPD44995.1"
                     /translation="MSTKSQTLKGFRDFLPSQMAVRDYVKKICVDTFELFGFQPLETP
                     ALEYSSVLMGKYGAEANKMIYSFKDKGGRDIGLRYDLTVSTARVLSSYNLPLPFKRYQ
                     IQPVWRADKPQKGRYREFTQCDIDTFGSCSPLADAEIIAVIYHVLTRLNFQKFSLRIN
                     SRTLLKNIPNCALQSIDKLGKIGNKRVQKELINKGYSQAQIDQFFEYIKTAQPDAYLN
                     QVFKRIIDFGVPAAAFTFDPTLVRGLDYYTGPIFEVYVEKPKIGSIAGGGRYDNLVRS
                     LGGPDIPAVGTTLGLDRIIDCILELNLLPQIRKTNIKVLVANFGEEKKSLELLNNLRK
                     NKIPSTIYFNKEKLVKQIKYALSLGAKYIAIIGPDEAKNNTITLKNLDTQDQQTISFQ
                     DLVIQLLTNA"
     gene            1742..2491
                     /locus_tag="PK131_02370"
     CDS             1742..2491
                     /locus_tag="PK131_02370"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF014098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HPD44996.1"
                     /translation="MIIKTIKTEKVTERNKGLVDFVDKNIKKLAERSVVAITSKVISI
                     LEGRIVKKKGINKADLVEAESEKYLVYTNDYCHHDFTLSIKNNLLIPNAGIDESNAFD
                     HYILWPKDPYKSARIIWDFLREKFKIKNLGVIITDSRTTPLRWGTTGVSIGFCGFEGL
                     NNYIGRRDIFGRKLKVTKANIADGLAAAAVVAMGEGEERTPLAIITDVKNIKFKGRAT
                     TKREMKSFRIAMTEDIYSPLLLGVRWIEGGE"
     gene            complement(2483..2556)
                     /locus_tag="PK131_02375"
     tRNA            complement(2483..2556)
                     /locus_tag="PK131_02375"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2520..2522),aa:His,seq:gtg)
BASE COUNT          823 a          449 c          640 g          822 t
ORIGIN      
        1 agcctacttt attagcgtaa cgggtataca ttcgttttag gtcattggcc caaagctggg
       61 cctcctcacc gccaggacca gcgcgaaact ccattatagc gatattagga tcaacggtcg
      121 gcatacttta ataaattcca aattattttt tacccttttt agtttttttg gtataacttt
      181 tacccttagc gactttttcg ttaaatttat caattctacc ccgaacgtca acaaattttt
      241 gctgaccggt ataaaacgga tgacagtggc tgcacacttc aacctcaatt gtctcttggg
      301 ttgaaccagt agtaaattga taaccacagc tgcaatttac cttggcttga tgataatatt
      361 ttgggtgaat tccttgtttc ataggagtta ttgtagcata ttattttaat aataataccg
      421 atattggtag atgttgctag acaatgacag ggtattttcc gaaggaaatc ggaaagttcc
      481 agcatttaat aaggatttta ggcatttgta agaagttgaa tgactaaatc ttgaaaactg
      541 atggtttgct ggtcttgggt gtcaagattt tttagagtga tagtattgtt tttggcttcg
      601 tccgggccga taatagcgat atacttagcg cctagagata gcgcatattt gatttgtttt
      661 accagctttt ctttattgaa gtaaatagtt gaggggattt tgtttttcct tagattattt
      721 aacaactcga gtgatttttt ttcttcgccg aaattggcca ccagaacttt aatatttgtt
      781 ttccggattt gagggagtag atttagttcc agaatgcagt caataattcg gtccagaccg
      841 agggtagtgc cgacggctgg aatatcaggt ccgcctaaag atcggactaa attatcgtag
      901 cggccgccgc cagcgataga gccgatttta ggtttttcaa catagacttc gaaaatcgga
      961 ccagtgtaat aatcgaggcc acggactaaa gttggatcga aagtgaaagc ggcggcgggg
     1021 acgccaaaat caataattct cttaaaaacc tggtttaaat aggcgtctgg ttgagctgtt
     1081 ttgatatatt cgaagaactg atcaatttga gcctgggagt aacccttatt aatgagctct
     1141 ttttgtactc ttttattacc tattttccct aacttatcga tagattgtag agcgcaattc
     1201 gggatatttt tgaggagagt tcggctatta atccggagag agaatttttg aaaattcaac
     1261 cgggtcagaa catggtaaat gacggctatg atttctgcat cggcgagggg agagcaagat
     1321 ccaaaagtat caatgtcgca ttgagtgaat tctcgataac ggcctttttg gggcttatca
     1381 gcccgccaaa ccggctgaat ttggtagcgt ttgaaaggca aggggaggtt ataactagag
     1441 agaactcggg cagtggagac agtgaggtca tagcgcaaac caatatcgcg gccgccttta
     1501 tccttaaaag aataaatcat tttgttagcc tctgcgccat attttcccat taataccgac
     1561 gaatattcaa gagctggggt ttccagcggc tggaagccga aaagctcgaa agtatcaaca
     1621 cagatttttt tgacatagtc gcgcaccgcc atttgcgacg gtaaaaaatc ccggaaaccc
     1681 tttaatgttt gcgatttggt tgacatggct aattttagca tttgtgggtt aaaatagggt
     1741 ggtgattatt aagaccataa aaacagaaaa ggtgacggag agaaataaag ggttggttga
     1801 ttttgtggat aaaaatatta agaaattggc tgaaagatcg gtggtagcaa ttacctcaaa
     1861 agtaatttca attttggaag gaaggatagt aaaaaagaag ggaattaata aagccgattt
     1921 ggttgaggca gaaagtgaaa aatatttggt ctataccaat gactattgtc atcatgattt
     1981 tactttgtcg attaaaaata atttactgat tcctaatgct ggcattgacg aatcaaatgc
     2041 ttttgatcat tatattttat ggccaaaaga tccgtataaa tcagctcgaa ttatttggga
     2101 ttttttgagg gaaaaattta aaattaaaaa tttgggggta ataatcaccg atagtaggac
     2161 gaccccctta cgctggggaa caactggcgt tagtattggt ttttgcggct tcgaagggct
     2221 aaataattat atcggccgga gggatatttt cggccgaaaa ttaaaagtga cgaaagcaaa
     2281 tatagccgat ggattggcgg cggcggcggt agtagcgatg ggggaggggg aagagcggac
     2341 accgctggcg attattaccg atgttaaaaa tattaagttt aaaggaagag caacgaccaa
     2401 gagagaaatg aaaagtttta ggattgctat gaccgaagat atctattcgc cgctattatt
     2461 gggagtaagg tggattgaag ggggtgagta accggaatcg aaccggcaac cttctgctcc
     2521 acaggcagac gctctaacca gttgagctat acccaccagg gaaatttgct agtgcaaatt
     2581 taatttttaa tcccaaattt taaattaaaa actgtctcat tttaatcgag agaaagggat
     2641 ttatcaaggt gttatgttaa gattacttat gaagctgatc gaaaatggtt gggagttagg
     2701 aatcgattta atgggaggac gaattacgga atta
//
LOCUS       DAOOIE010000075         2630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_30395_length_2630_cov_3.426408, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000075 DAOOIE010000000
VERSION     DAOOIE010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30395_length_2630_cov_3.426408"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1114)
                     /locus_tag="PK131_02380"
     CDS             complement(<1..1114)
                     /locus_tag="PK131_02380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPD44997.1"
                     /translation="MEINRKDHSLYSVAGFFSYAVAQLAGAMKKIKSINHKRFWLGFL
                     KVVLVLLGVSIVGLIGLFVWYSRDLPAPDRIVRREGYASKVLDRNGEIIFDLYKEAKR
                     TPVAWEEIPDALKKATIAVEDKDFYKHRGFDPLTPARIAKNFFLTGRLIGGSTLTQQL
                     VKNVLLTSERTITRKIKEFILAVQIEARYKKDDILLMYLNEAPYGGMAWGVGSAAEQY
                     FGKKVHDLDVAESVILAGLPQRPSVYSPFSQTPTAYIDRSKHVLERMQEDGYLTADEV
                     SQVFEEIKNYQFYQNKSKNFAPHFVDWIKDRLIKNYGEAVMDIGGLTITTTLDLKLQG
                     EMQTIVSDEIDKAEKQGISNGAAIAVDPRTGEVLAMI"
     gene            1156..>2630
                     /locus_tag="PK131_02385"
     CDS             1156..>2630
                     /locus_tag="PK131_02385"
                     /inference="COORDINATES: protein motif:HMM:NF028500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPD44998.1"
                     /translation="MLPGIGPKTIQKLAKLKITSPTDLIYHFPHRYLDFSHIKSIAAV
                     QVGENVTITGKITQFHNIFTRSQKNIQKAVIRDQTGYLNLIWFNQPYLSQSIKVGETY
                     SFAGEISLYQNQKTLIAPVYGQYHTGKIIGIYPETVGLSSNWFRKTIQNYLPFLLSSI
                     TDPLPTDLLKKYHLLDLKTALRQIHQPTNLAILNQARFRLSLDEILSIQSDSYQQKQT
                     WLTLKPAVILADKSVDSLINRLPFHLTSGQIKVWHEIKADLLSNQKPMNRLLQGDVGA
                     GKTVIAMLAAYLTAQNNSRTIFMAPTEILAQQHFQTISAYLKNTYLLTAKTKLDLEKI
                     PQNAVIISTHAIIYRQKITNVGLLVIDEQHKFGVKQRSFLSGLNPPHTLTMTATPIPR
                     TISLTFLGNLDLSTIETLPQNRLSIKTFVVPQSKKRDCYRWIDQQIKTTKEQAFIVCP
                     FINESENLTTVKSAQAEFEKLQKVFPDQKLALIHGKIKPVTR"
BASE COUNT          832 a          634 c          399 g          765 t
ORIGIN      
        1 caatcatagc taaaacttcg ccagttcggg gatcaactgc tatagccgct ccattagata
       61 tcccctgttt ttcggcctta tcaatctcat cactgacaat agtctgcatt tcaccttgta
      121 attttaagtc caaagtagtg gtaatggtta agccgccaat atccatcacc gcctcaccat
      181 aatttttaat taatcggtct ttaatccaat caacaaaatg cggcgcaaaa tttttactct
      241 tattttgata aaactgataa tttttaattt cctcaaaaac ctgactaact tcgtcagcag
      301 ttaaataacc atcttcctgc atccgttcta aaacgtgttt tgatcgatca atataagctg
      361 ttggtgtttg ggaaaaggga gaataaacac ttggccgttg aggcaaaccg gccaaaatca
      421 ccgattcggc tacgtctaaa tcatgaactt ttttaccaaa atactgctca gcggcgctgc
      481 caacccccca agccataccg ccgtatgggg cttcatttaa atacattaac aaaatatcat
      541 ctttcttata tctagcttca atctgaaccg ccagaataaa ctcttttatc tttctggtaa
      601 tagtccgctc gctggttagg agaacatttt taaccaattg ctgggtcaaa gttgaaccac
      661 caatcaatcg gccggtaaga aaaaaatttt tcgcaattct agccggagtc aaaggatcaa
      721 aaccccgatg tttataaaaa tctttatctt ctacagcaat ggtagctttt ttgagagcat
      781 caggaatttc ttcccaagca accggcgttc ttttagcttc cttatataaa tcgaaaataa
      841 tctcgccatt tctatccaaa actttactgg cataaccttc ccgcctaaca atccggtcgg
      901 gtgccggtaa atcccgcgaa taccacacaa ataatccaat cagcccgact atagataccc
      961 ccaataagac taaaactacc ttcaaaaaac ccagccaaaa ccgtttgtga ttaatcgatt
     1021 taattttctt cattgcgcca gcaagttgag ccacggcata actaaaaaag ccagcaacgc
     1081 tataaagaga atgatctttt ctattaattt ccatgttttt ttcttaattc gcatcaatta
     1141 atgataacat aagttatgct tcccggtatc ggccccaaaa ccattcagaa actagccaag
     1201 ttgaaaatca ccagtccaac cgatttaatt tatcattttc ctcatcgtta tcttgatttt
     1261 tcccatataa aatctattgc cgctgttcag gttggtgaaa atgttactat taccggtaaa
     1321 attactcaat ttcataatat tttcactcgc agccaaaaaa acattcaaaa agcggtaatt
     1381 agggatcaaa ctggttattt aaatttaatt tggtttaatc agccttattt aagccaaagc
     1441 attaaggtag gggaaaccta tagttttgcc ggtgaaatca gtctttacca aaaccaaaaa
     1501 actctgattg ctccggttta cggccagtat cacaccggca aaattatcgg catctatccg
     1561 gaaactgttg gtttatcatc aaattggttc cgaaaaacta ttcaaaacta tttacccttc
     1621 cttttgtctt caattactga tcctctaccg actgacttac tgaaaaaata ccatctttta
     1681 gatctcaaaa ctgctcttcg tcaaattcac caaccgacca atctggctat tctaaaccag
     1741 gcaagattcc gcctgtcgtt agatgaaatt ttatcgatac aatccgattc ctatcagcag
     1801 aaacaaactt ggttaacctt aaaaccggca gtaattttgg ccgataaatc ggtggattcc
     1861 ctgattaatc gactgccttt tcacctaacc agcggccaaa ttaaagtttg gcacgaaatt
     1921 aaagctgatc ttttatctaa tcaaaaacca atgaaccggc ttctgcaggg cgatgttggc
     1981 gccggcaaaa ccgtcatcgc tatgctggcc gcttatctaa cggcccaaaa taacagccga
     2041 acaattttca tggctccgac cgaaattcta gctcagcaac attttcaaac catttcagct
     2101 tatctaaaaa acacctactt attaacagcc aagactaaac ttgatttaga aaaaattccc
     2161 caaaatgctg ttattatttc cacccatgcc attatttacc gccaaaaaat taccaatgtc
     2221 ggcctgctag taattgatga acagcataaa tttggcgtaa aacagaggtc tttcctcagc
     2281 ggccttaatc cgccccatac tctaaccatg accgctactc ccattccccg aactatcagc
     2341 ttaacctttt tgggcaatct tgatctatct actattgaaa ctttgcccca aaatcggctg
     2401 tcaattaaaa cttttgtcgt cccccaatcg aaaaagagag actgctaccg ctggatcgat
     2461 cagcaaataa aaactaccaa agaacaagct tttattgttt gcccatttat caatgaatca
     2521 gaaaacttaa ctacggttaa gtcggctcaa gccgagttcg aaaaactgca aaaagttttc
     2581 cccgatcaga aattagccct aattcacggc aaaattaagc cggtaacccg
//
LOCUS       DAOOIE010000076         2592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_31018_length_2592_cov_3.130469, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000076 DAOOIE010000000
VERSION     DAOOIE010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31018_length_2592_cov_3.130469"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="PK131_02390"
     CDS             <1..832
                     /locus_tag="PK131_02390"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPD44999.1"
                     /translation="TCSLKLSKISDNGVEFTSHIDGSKHFFTPEKSIQIQHNLAADYL
                     VAFDDCAPYPAKKEDVREALGRTHRWALRSLAEHQKLNRKLHQNLKLYGVIQGGCFKD
                     LRQESAKFICDQNFDGIAIGGISVGESKEKMRQVVDWILPTILKTKKPIHLLGVGEID
                     DLFHFFGQGITSMDCTLPTRLGRAGYALSRQAGFKFKISNLRLKSDSAPLDPDCHCYV
                     CQNYSRAYIHHLFKAQELLSYRLLTYHNLSFMINLQNQIRKALKNGTFLQLKREWLGC
                     "
     gene            849..1718
                     /locus_tag="PK131_02395"
     CDS             849..1718
                     /locus_tag="PK131_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002678373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPD45000.1"
                     /translation="MKTILVTGGAGHVGSHVIEELVKDPNNKVISLDNYFNGSIHNHI
                     RGAEYRRGHTKNIDKLIPETPDIIYHLGEYARTTPSFNEVEKVYDMNIIGTFAVAEFC
                     RKRKVGKLVYAASSTKFAIEGDGRHQNPYSFTKAVNVDLINDYGRWYQLPHAICYFYN
                     AFGPRENGVGKYATLIAKFEQAYLHNQELPVIKPGTQKRNFTYVKDLAKGIILVGEKG
                     QGDGYTLGNTKGYSIIEIAEAFGGPIHFIDGYPGRLESGEAPDKARQELGWQTTIGIM
                     EYIKDFIRKHPRQ"
     gene            1715..>2592
                     /locus_tag="PK131_02400"
     CDS             1715..>2592
                     /locus_tag="PK131_02400"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPD45001.1"
                     /translation="MKINSQRSLIKLLKSSSPKTFRVFNFGCRVNAAEINQLSQFFIN
                     YGFTPSLVDNPQIILINTCAVTRKGENESLTKIRVLQKQYPQSLIIATGCANLNKIQN
                     ILTIDNILKDNILTNTNGYYSPKIGDKYTPHKRYLLRVQSGCTQKCSYCIVPQKRPHL
                     WSLAPSTAVATIKKIEAEYNEIIITGVNLVQYQYGLTDLVKKIIEETKIKLISFGSIP
                     LNCFNDEFLNLLDNKRIVKFLHIPIQSGSNRILKLMRRPYNQKKIINVFESLKSKIKN
                     LSLGTDIIVGFPTETKA"
BASE COUNT          835 a          531 c          464 g          762 t
ORIGIN      
        1 tacatgttcc cttaaattat ctaaaatttc tgataatggg gtagaattta cttcccatat
       61 tgatgggtca aaacattttt ttactccgga aaaatcaatc caaattcaac ataatttagc
      121 cgctgactac ctggttgcct tcgacgattg tgctccttac ccagccaaaa aggaagatgt
      181 tcgggaagcc ttaggccgaa ctcatcgctg ggccctacgc tctctagccg aacaccaaaa
      241 actcaaccga aaattacatc aaaacttgaa actatacggt gtaattcaag gtggctgttt
      301 taaagattta aggcaagaat cggccaaatt tatctgtgat cagaattttg acggaattgc
      361 cattggcggt atttcagtcg gtgaatctaa ggaaaaaatg cgtcaagtgg tggattggat
      421 tttaccaact attttaaaaa ccaaaaaacc aatccatctc ctgggagtgg gggagattga
      481 tgatctgttt cattttttcg gccaagggat cacttcgatg gattgcaccc tgcctaccag
      541 actcggtcgc gccggctacg ctcttagccg acaggcgggt ttcaaattta aaatttcaaa
      601 tttaagatta aaaagtgact ctgccccgct cgaccccgat tgtcactgtt acgtttgcca
      661 aaattacagt cgggcctata tccatcatct ctttaaagct caggaattat tatcttatcg
      721 tcttcttacc taccacaatt tatcttttat gatcaacctt caaaatcaaa ttagaaaagc
      781 gcttaagaat ggcacttttc tacaattaaa acgggagtgg ctgggctgtt aatttgatat
      841 aatagttcat gaaaactatc ttagtaacag gaggcgctgg tcatgttggt tcacatgtca
      901 tcgaagaatt agtcaaagac ccaaacaaca aagtaatctc cctcgataat tattttaacg
      961 gcagtattca taatcatatt cgtggagccg aataccgccg gggccatacc aaaaatattg
     1021 ataaattaat tcctgaaaca ccggacataa tttatcattt gggtgaatat gcccggacga
     1081 ctccatcttt taacgaggta gaaaaagtct atgacatgaa tattattggc acttttgccg
     1141 tggccgaatt ctgccgaaaa agaaaagtgg gtaaattggt ttacgccgct tccagcacca
     1201 aattcgctat cgaaggcgat ggccgtcacc aaaatcctta ttctttcacc aaggctgtta
     1261 atgtcgattt aattaatgac tatggccgct ggtaccaact tcctcatgcc atttgctact
     1321 tctataatgc cttcggtcca agagaaaatg gagtcggaaa atatgccact ctcatcgcta
     1381 aatttgaaca agcctactta cacaaccaag aactccccgt aattaaaccg ggtactcaaa
     1441 agcgaaattt cacctatgtt aaggatttag ccaaggggat tattttagtt ggagaaaaag
     1501 gtcaaggcga cggttacacc ttgggcaaca ccaagggata ctccattatt gaaattgccg
     1561 aagctttcgg cggtccaatt cactttatcg atggttatcc gggcagatta gaatcgggag
     1621 aagcaccgga taaagcccgt caggaactgg gctggcaaac aaccatcggc attatggaat
     1681 atattaaaga cttcattcgg aaacatccgc gccaatgaaa attaactccc aaagaagttt
     1741 aatcaaacta ttaaaatctt cttcacccaa gactttccgg gttttcaatt ttggctgccg
     1801 ggttaacgcc gctgaaatca atcagttatc tcaatttttt atcaattatg gttttacccc
     1861 atctttagtt gataatcctc aaattatttt aatcaacact tgtgctgtta cccgaaaagg
     1921 agaaaatgaa tcgttgacta aaattcgggt tcttcaaaaa caatatcccc aatcattaat
     1981 tattgccact ggttgtgcta atttaaataa gattcaaaac atattgacaa tcgacaatat
     2041 actaaaggat aatatattga ctaataccaa tggttattat tcaccaaaga taggagataa
     2101 atatacgccg cataaaagat atcttctcag ggttcagtct ggttgtactc aaaaatgctc
     2161 ttactgtatt gtccctcaaa aacggccgca tttatggtcg ctcgctccat caactgcagt
     2221 agcaactatc aaaaaaattg aggcggaata taacgaaatt attattacgg gagttaattt
     2281 agtccaatat caatatggtt taactgattt ggtcaaaaaa attattgaag agactaaaat
     2341 aaaactgatc agttttggtt ctattcccct caattgtttc aatgatgagt ttttaaacct
     2401 attagataat aaaaggatag tcaaatttct tcatattccc attcagtcag gtagtaatcg
     2461 gattttaaaa ttaatgcgcc gcccctataa tcaaaagaaa atcattaacg tttttgaaag
     2521 tttaaaatca aaaatcaaaa atttatccct tggtaccgac attatcgttg gttttcccac
     2581 tgaaactaaa gc
//
LOCUS       DAOOIE010000077         2588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_31095_length_2588_cov_2.898144, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000077 DAOOIE010000000
VERSION     DAOOIE010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31095_length_2588_cov_2.898144"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PK131_02405"
     CDS             complement(<1..280)
                     /locus_tag="PK131_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HPD45002.1"
                     /translation="MKNTWLITKKELMDYFYSPRGYLVTGAMITVANWLFFKNIFLIN
                     QAEMSNYWETMAFLLSLLIPAMTMGLFAEEKKNHTWEILNSLPLKIKDI"
     gene            complement(277..957)
                     /locus_tag="PK131_02410"
     CDS             complement(277..957)
                     /locus_tag="PK131_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPD45003.1"
                     /translation="MIELKNISKSFGEIKALDKVSFKLPSEGVIGLLGPNGAGKTTLM
                     RILVGYLQPDQGKILWEGKRVNPNSIKHKSRLGYLPENNPLYDNMTIKEYLSFIASIR
                     NAAEEQINKVVRECGLKNVIQQKIEILSRGYKQRVGLAAALLGKPKLLILDEPTSGLD
                     PNQIIEIRQLIKRLAVKKLIILSTHILPEAREICQRILIISQGRIVLDADPKSIKDLE
                     KKFVELTG"
     gene            complement(998..1852)
                     /gene="era"
                     /locus_tag="PK131_02415"
     CDS             complement(998..1852)
                     /gene="era"
                     /locus_tag="PK131_02415"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HPD45004.1"
                     /translation="MFKYGTAAIIGRPNTGKSTLLNAIMNQKVTITSPLPQTTRKNVR
                     VRYQDERGAIDFIDTPGMMGKIEDLVGKKANRETPKSLSGADVVICLMDISRPKNEED
                     NKVIGLVRKSTAPKILVYNKIDKAKVSQGYLPEYNFLEEEFDHVVGISAIRNKNVREL
                     IKIIFELLPEGQPQEDQSEFKIPVSSSDYISEIIREKLFLFLRREVPYTTTVVVDSIE
                     DKKAIIVVKADILTNAERYKKMIIGKNGQKIKQIGSNARKELELMSGRKIYLDLTVKI
                     DKHWQERV"
     gene            1934..2440
                     /locus_tag="PK131_02420"
     CDS             1934..2440
                     /locus_tag="PK131_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nicotinamide nucleotide
                     adenylyltransferase"
                     /protein_id="HPD45005.1"
                     /translation="MLYFAFPGCFNPPTLAHLAIAKSILKKYPDSKVIFIPVSDYYLK
                     KDLAPAKDRCQMLKLVVGKNMIISDIEIKSKFQPKTLVTLNKLKRNYPNLRLLIGSDN
                     YRRIKTWYHYQTLLKKYSPEIVKRNSSRLLTISSSKIRSLIKRGQSCSRYLDPKVIKY
                     INDHRLYR"
     gene            2418..>2588
                     /locus_tag="PK131_02425"
     CDS             2418..>2588
                     /locus_tag="PK131_02425"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HPD45006.1"
                     /translation="MTIDYIVKKIKQFYQANNFQKAIIGLSGGIDSAVTAALLVKALG
                     SDQVLAAYLPNNN"
BASE COUNT          747 a          511 c          440 g          890 t
ORIGIN      
        1 caatatcctt tatttttagt ggcagagaat taaggatttc ccaggtatga ttcttttttt
       61 cttcggcaaa aagacccatc gtcatcgccg gaataagaag cgatagtaaa aatgccattg
      121 tttcccaata atttgacatt tcagcctgat taatgagaaa aatatttttg aaaaaaagcc
      181 aattagccac cgtaatcata gccccagtga ccagataacc ccgtggacta taaaaataat
      241 ccattaattc ctttttagta attaaccaag tatttttcat ccagttaact ccacaaattt
      301 tttctctaaa tctttaatgg atttggggtc agcatctaaa acaattcgac cttgcgaaat
      361 aatcagtatc ctttgacaga tttcccttgc ttctggcagg atatgggttg ataagataat
      421 tagttttttt actgctagtc ttttaatcag ttgtctgatt tcgataatct gattcggatc
      481 taaacccgaa gtgggttcat ctaaaatcaa taacttgggt ttaccgagga gagctgccgc
      541 taaaccaact ctttgtttat aaccccgcga taaaatttct attttttgct gaatcacatt
      601 tttaagaccg cattctctga caactttatt aatttgttct tcggcggcat ttctaatgct
      661 ggcaataaaa gataaatatt ccttgatagt catattgtca tagagagggt tattttccgg
      721 caagtagccc agacgtgatt tgtgtttaat ggaattagga ttaactcttt ttccctccca
      781 gagaatttta ccctgatcag gttgaagata gccaactaaa attcgcatca gagttgtttt
      841 accggcgcca tttggtccca aaagaccaat gacgccctcg cttggcaatt taaaactaac
      901 cttatctaag gcctttattt ctccaaaact cttgctaata ttttttagct ctatcatctt
      961 ttaattcgta attcctaatt tttaatttgt aattatttta taccctctct tgccaatgtt
     1021 tatctatttt aacagttaaa tcaagataga tttttctgcc cgacattaat tctagttctt
     1081 tcctggcatt actgccaatt tgcttaattt tttgaccgtt tttgccaatg atcatttttt
     1141 tgtaacgttc agcattggtt aaaatatcgg ccttaacgac gattattgcc tttttatcct
     1201 cgatactatc tactacaact gtagtagtat agggaacctc tcgccttaaa aaaagaaata
     1261 gtttttctcg gataatttca gaaatataat cactggaact aactggaatt ttaaattcgc
     1321 tttgatcttc ttgaggttgg ccttcaggca acaactcaaa gataattttg attaattccc
     1381 gaacgttttt gttcctaata gctgaaatac cgacaacatg gtcaaattct tcctctaaga
     1441 aattgtactc tggcaggtag ccttgactaa ctttagcttt atctattttg ttatagacta
     1501 aaatttttgg ggcagttgat tttctcacta aaccgataac tttattatcc tcctcatttt
     1561 tgggccggct gatatccatt aagcaaataa caacatcggc gccggacaat gatttcggcg
     1621 tttcccgatt ggcttttttt ccgactaggt cttcaatttt ccccatcatt cccggggtat
     1681 cgatgaagtc gattgctcct ctttcatctt ggtagcggac ccgaacgttt tttctggttg
     1741 tttgagggag gggagaggtg atggttactt tctgattcat gatggcatta agaagggtgg
     1801 attttcccgt attcggccgg ccgataatag ccgcagtacc atatttgaac atattgtcca
     1861 ttataacaaa tttccccttt tccagccgaa ggcgggagag gggtttaagc gctataatag
     1921 ttataatatt cttatgcttt actttgcttt tcctggctgt tttaatccac caactctagc
     1981 ccacctagct attgccaaat ctattttaaa aaaatacccg gattccaagg taattttcat
     2041 tccagtatca gattattatc taaagaaaga tttagctccg gccaaggacc gttgccaaat
     2101 gcttaaactg gtggtgggca aaaacatgat tatcagcgac attgaaatta aaagtaagtt
     2161 tcagcctaaa accttggtta ctttaaataa actaaagaga aattacccta atttaaggtt
     2221 attaattggt tccgataatt accggcgaat taaaacctgg tatcactatc aaactttact
     2281 gaaaaaatat tccccggaga tagttaaaag aaacagttct cgtcttctta ccattagttc
     2341 cagtaaaatc cgctctttaa ttaagcgggg acaatcttgc agccgctatt tagatccgaa
     2401 agttataaaa tatattaatg accatcgatt atatcgttaa gaagattaaa cagttttatc
     2461 aggctaataa ttttcaaaaa gcaattatcg gtctgtctgg ggggatcgat tcagcggtaa
     2521 ctgccgctct attggttaag gctttggggt ccgaccaagt tttggccgct tatctgccca
     2581 acaataat
//
LOCUS       DAOOIE010000078         2558 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_31629_length_2558_cov_2.731922, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000078 DAOOIE010000000
VERSION     DAOOIE010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31629_length_2558_cov_2.731922"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..439
                     /locus_tag="PK131_02430"
     CDS             <1..439
                     /locus_tag="PK131_02430"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPD45007.1"
                     /translation="KLFKTQIELAKKYNLPLIIHARKAVDETIEILKNYSSLRGVFHC
                     YAGGRKRIKNILALGDNWYFGIDGNLTYEIGLEQVVLNIAKDRLILETDCPWLAPVPH
                     RGEENKPAYVKYVYQKVAEIWQMSFEETEKIIDGNAKKLFSIP"
     gene            complement(429..671)
                     /locus_tag="PK131_02435"
     CDS             complement(429..671)
                     /locus_tag="PK131_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45008.1"
                     /translation="MRIQLYDRGSPHGKKMFADIQNICRRLQIDDDPEYIQEMTRPYS
                     MGIQGKTILMINGGAVLVDEFLSEKELEAIIMDFME"
     gene            complement(864..1727)
                     /locus_tag="PK131_02440"
     CDS             complement(864..1727)
                     /locus_tag="PK131_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45009.1"
                     /translation="MNKEIQKEIQEMAAAAAVTTVETRQWASEVLQLENDAWREVLGR
                     NVDIAPLPSIVTPEVKRNLEHLGLGLRFIPKMDIGTLDILKKKGTVGYLDELQSLYPG
                     WKRYESLSNTERRYHSVGRNLNEWYWNKVECGHIDFPRLPGVWVAVETMPKPSCGDKY
                     EKTAITDRLRLPDRFGVAWNNAQTAIQRAKRPILLESGLPGSLDVRMLEALEWNLLAN
                     REGWGATNTYEWTNTKYRKYCWWGDFYRVFIGCSTCGGAARAGCFSRDCPVDVIGFRL
                     AVILDSRSLKT"
     gene            complement(1773..>2558)
                     /locus_tag="PK131_02445"
     CDS             complement(1773..>2558)
                     /locus_tag="PK131_02445"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HPD45010.1"
                     /translation="LKDTIRKNKLRPVLDMILVGDNPASIKYTSLKQATGLSIGIGGT
                     IHHLAENTSESAVIDLIKKLNHNPQVTAVMVQLPLPKGFNTEKIINTIDPTKDADGLT
                     AVNLGRLFQKDKTVLVSATPLGIITLLDRYKVNLCGKYAVIINRSPFIGLALMALFLN
                     RDATVSLCHSQTSNVRKLSRQADIIISGTGQSGYLTADYIRKGAVVIDVGMEVDFAKV
                     KDKCSFITPPIGGVGPMTVASLLANTVKIALKSSHFDSPNNRL"
BASE COUNT          789 a          548 c          492 g          729 t
ORIGIN      
        1 caaacttttt aaaacgcaaa tagaactggc taaaaaatat aatctaccgc taattattca
       61 cgcccgaaag gcggttgatg aaacaattga aattttaaaa aattattcca gtttaagggg
      121 cgtttttcac tgctatgccg gcggcaggaa gcggattaaa aatattttgg ccttgggaga
      181 taattggtat tttggcatcg acggcaacct aacttatgaa atcggactgg agcaagtggt
      241 gttaaatatt gccaaggata ggttaattct ggaaacggat tgcccttggc tggcgccggt
      301 tccccatcga ggcgaggaaa acaaaccagc ctatgttaaa tatgtttacc aaaaagtggc
      361 agagatatgg caaatgagtt ttgaagaaac ggaaaaaatt atcgatggga atgcgaaaaa
      421 attattttct attccataaa atccataata attgcttcca gttctttttc ggagagaaat
      481 tcatcaacta gaacggcgcc gccgttaatc atcaaaatag ttttgccctg aattcccata
      541 gaataaggtc tagtcatctc ctgaatatat tcaggatcgt cgtcaatttg aagccgccga
      601 cagatatttt ggatatcggc aaacattttt ttaccatggg gactgcctcg atcataaagc
      661 tgaattctca ttagtttatt ttaacccaaa tttattttta attccaaata tttgaaataa
      721 ctcatagaga acaggtatgc ctgtttccca cgacatgaat ataaaatgtt atgtttaggt
      781 agagagggat ttcaaagcaa ttttcaccgt gttcgctagc aaagaggcta ccgtttatca
      841 agtattatgg gaaatgcctg aattcaagtt ttaagcgatc gggaatcaag aattacggcg
      901 agacgaaacc cgataacatc aaccgggcag tcacgactaa aacagccggc gcgagcagca
      961 ccaccgcatg tagagcaacc aataaagaca cggtaaaagt caccccacca acaatattta
     1021 cgatattttg tatttgtcca ctcgtaggtg ttagttgctc cccatccctc acggttagcc
     1081 aataaattcc actctaaagc ctcaagcatt cttacatcca ggctgcctgg aagtccagat
     1141 tctaaaagaa ttggcctttt tgccctttga atggcagttt gagcattatt ccaggcaaca
     1201 ccaaatcgat caggaagcct caatctatcg gtgatagctg tcttttcata cttatctccg
     1261 caagaaggct taggcatggt ttcaacagct acccagacac ctggaagccg aggaaaatca
     1321 atatgtccac attctacctt attccaatac cactcattaa gatttctgcc aaccgaatga
     1381 tatcttcgtt ctgtattact caacgactcg tatcgcttcc aacctgggta aagtgactga
     1441 agttcatcaa ggtatcctac tgtgcctttc ttcttcaaga tatcaagtgt tccaatatcc
     1501 attttgggaa taaaacgcaa tccaagcccc aagtgctcca aattcctttt aacttcaggt
     1561 gtaactattg aaggcagagg agcaatatcg acatttctcc ctaagacctc tcgccaagcg
     1621 tcgttctcta attgaagcac ctctgatgcc cattgtcttg tttctactgt tgtgaccgct
     1681 gccgcggcgg ccatttcctg tatttctttc tgtatttctt tattcattag tgatattagt
     1741 attataatag gacaaagaaa tcaacagaca gatcaaaggc ggttattagg cgaatcgaaa
     1801 tgggaggatt tcaaagcaat tttcaccgtg ttcgctagca aagaggctac cgtcatcggg
     1861 ccgacgccgc caatgggagg ggtaataaaa ctacatttat ccttaacttt agcaaaatca
     1921 acctccatgc ccacatcaat taccaccgct ccttttctaa tataatccgc agtcaagtaa
     1981 cctgattgac cagtgccgga aataataata tctgcctgcc ggctcaattt tctaacgtta
     2041 gaggtttgag aatgacaaag gctgacagta gcatcccgat taagaaaaag agccattaag
     2101 gctagcccaa taaaaggact gcgattaata attaccgcat atttaccgca aagattaact
     2161 ttatatctat ccaaaagggt aataattccc aagggggtag ctgaaaccaa aactgtcttg
     2221 tctttttgaa aaaggcggcc aagattaacg gccgtcagac cgtcggcgtc cttagtcgga
     2281 tcaatagtgt taataatctt ttcggtattg aaacccttgg gtagaggcag ttgaaccatt
     2341 accgcggtaa cttgaggatt atgattcaac tttttaatca gatctattac agccgattca
     2401 ctggtatttt cagctaaatg atggatagta ccaccaatac cgatagacaa accggtagcc
     2461 tgttttaagg aagtatattt aattgaagct ggattatccc ccaccaaaat catatctaaa
     2521 accggcctta atttattttt tctgatggta tcttttaa
//
LOCUS       DAOOIE010000079         2556 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_31666_length_2556_cov_3.152339, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000079 DAOOIE010000000
VERSION     DAOOIE010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31666_length_2556_cov_3.152339"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="PK131_02450"
     CDS             complement(<1..997)
                     /locus_tag="PK131_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45011.1"
                     /translation="MKRKGAVQLSFILGLLILTIALGLVSYLVQQPQEVRKRAEEQLP
                     GILAETDLPAMASYGRADDCQDYTYGLTYNFADYNGDSYQDLVIESGRINDSGNRCLS
                     FWANKKDGSLKKEGRSYRWSKKWDEISYVTGVWGCGGNSWNYLEKKDTYSYAGADCAL
                     VGMNLAIGDTIGKTGEDRTGWKESVLSGTTGSLSSCLDYDSDGETEVLALNSKGEKSG
                     YKGVVVTWDKNNNAVSTRWTSTHLAYGSYWTGKAHLGSVDGEKIQNDFVLYDRLFRSV
                     MVFTSQKGLVKEIANFFDIGSTNAAEALGDVNGDGLDDLVYFVNPGTKPQLVFAPA"
     gene            complement(1036..2088)
                     /locus_tag="PK131_02455"
     CDS             complement(1036..2088)
                     /locus_tag="PK131_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HPD45012.1"
                     /translation="MDLLIFLIALSVLVLVHELGHYFFAVRSGVKVEEFGLGLPPRII
                     SRKIGQTVFSLNWLPIGGFCKLYGEEEEGKGKKAFNNKTPWQKFWVVIGGVLMNLLLA
                     GIIFSGVYLIIGGPKETNRVKILAVVPNSPAAVAGLQEGDWIKAINNEIIEKPAELID
                     LTEKIKGKKAELIIEREGMEKKVDVEVRVQPPEGEGAMGVAISNIEMQKIRWYEFYRG
                     IGYGFEEAYYWGKIIVGGVVRVVSDLFHGETPKDMAGPIGMYEATSIIKRNQGILAVI
                     HFFGVVSVNLAVVNILPFPALDGGRIIFVLYEMITKRKPNKELEIAINSIGMWLLLLL
                     IFVVTVGDIKRLVLKQ"
     gene            complement(2092..>2556)
                     /locus_tag="PK131_02460"
     CDS             complement(2092..>2556)
                     /locus_tag="PK131_02460"
                     /inference="COORDINATES: protein motif:HMM:NF013891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-recycling factor"
                     /protein_id="HPD45013.1"
                     /translation="ATPGLIEKVTVTVYNGQKMKLVELGSISVADARTLTFQPWDESI
                     IREISNGIMAANIGLTPAINGPVIHLTLPLMTVEQRDDYLKLLGRKLEGARVMMRDAR
                     ADYRKKLIEAKNEKIISEDEFKRDEMELQKITDEYIGKLENLAGKKELEIRG"
BASE COUNT          786 a          675 c          331 g          764 t
ORIGIN      
        1 ccgccggggc aaaaactaat tggggttttg ttcccggatt gacaaaataa accaaatcat
       61 ccaaaccatc accattaaca tcgcctaaag cttcggcggc attggtactg cctatatcaa
      121 aaaaattagc gatctcttta actagaccct tttgggaagt aaaaaccatt acactccgga
      181 ataaacgatc atatagaaca aaatcatttt ggattttttc cccatcaaca ctccccaagt
      241 gggctttccc ggtccaataa gacccataag caagatgggt cgaggtccat ctagtactga
      301 cagcattatt atttttatcc caggtaacta ccaccccctt ataacctgac ttttcgcctt
      361 tactatttaa ggctaaaact tctgtttctc cgtccgaatc ataatctaaa caggaagata
      421 aagagccagt cgtgccagaa agaacggatt ccttccaacc agtcctgtcc tctccggttt
      481 taccaatagt atccccaatg gccagattca tccctactaa agcacagtct gctcccgcat
      541 aggaataagt atccttcttt tctaaataat tccatgaatt gccgccgcaa ccccaaactc
      601 cagtaacata actaatttca tcccattttt tgctccatct atatgaccta ccctccttct
      661 tcaaggaacc atctttttta ttcgcccaaa aactaagaca acgattacct gaatcattaa
      721 tcctaccaga ttcaataact aaatcttggt aactatcacc gttataatcg gcaaaattat
      781 aagttaaacc ataagtataa tcctgacagt catcagctcg gccatagcta gccattgccg
      841 gcaggtctgt ttcagccaaa ataccgggta attgttcttc tgctcttttc cgaacctctt
      901 gaggttgttg aaccaaataa ctaactaagc ctaaagctat agttaggatt aacaaaccta
      961 aaataaaaga caattgaacg gcacctttcc tcttcattga tactcttgat tatggcacaa
     1021 agataaagca gctgtctact gtttcaatac caatctctta atatcaccaa cggtaaccac
     1081 aaaaattaaa agcaataata accacatacc gatactatta atagctatct ctaattcttt
     1141 atttggcttc cgtttggtaa tcatttcata taaaacaaaa atgattctgc ccccgtctaa
     1201 agcaggaaaa ggcaaaatat taactacggc taaattaacc gatactaccc caaaaaaatg
     1261 aataactgct aaaatcccct gatttctctt aataatacta gtagcctcat acatcccaat
     1321 gggcccggcc atatctttag gggtttcgcc atgaaataag tcactaacta ctcgaactac
     1381 tccaccaaca ataatttttc cccaataata agcctcctca aacccatagc ctattcctcg
     1441 ataaaattca taccagcgaa tcttctgcat ttcgatattg gaaatagcta ctcccatggc
     1501 tccttcacct tccggcggct gaactctcac ttctacatct acttttttct ccatcccttc
     1561 cctttctatt atcagttctg cctttttccc tttaattttt tcagtcaaat caatcaactc
     1621 cgccggtttc tcgatgattt cattattaat tgctttaatc caatctcctt cctgtaagcc
     1681 agcaacagcg gccggcgaat tcggaactac cgccagaatc tttaccctat tagtttcttt
     1741 agggccacca ataattaaat acactcccga aaaaattatc cctgctaaaa gcaaattcat
     1801 taaaaccccg ccaataacta cccaaaattt ttgccatgga gttttattat taaaagcttt
     1861 tttcccctta ccctcttcct cctcaccgta aagtttacaa aaaccgccta tcggcagcca
     1921 atttaaacta aaaactgttt ggccaatttt tcgacttata attctcggcg gtaaacccaa
     1981 accaaactct tcaactttga cccctgatct aacggcaaaa aagtagtgac ccaattcatg
     2041 aaccaaaact aaaaccgaaa gagcgatcaa aaaaatcaac aaatccatat tttatcctct
     2101 gatttccagt tcttttttcc ccgccaaatt ttctaactta ccgatatact catcggttat
     2161 cttctgcaat tccatttcat cccttttaaa ttcatcttcc gaaattattt tttcattttt
     2221 agcttcaatt aatttttttc gataatcggc tctggcatcc ctcatcatca cccgagcacc
     2281 ttccaatttc ctccccagca gttttaaata atcatcccgc tgttctaccg tcattaaggg
     2341 caaagtcaaa tgaataactg gaccgttaat agccggggtc aaaccaatat tggccgccat
     2401 aattccatta ctaatctccc tgataattga ttcatcccaa ggttgaaaag taagtgttct
     2461 ggcgtcagca acactaatac tccccaactc aactaacttc attttttggc cgttataaac
     2521 cgtgaccgtc accttttcga ttaaccccgg ggttgc
//
LOCUS       DAOOIE010000080         2494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_32808_length_2494_cov_3.043050, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000080 DAOOIE010000000
VERSION     DAOOIE010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32808_length_2494_cov_3.043050"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(326..700)
                     /locus_tag="PK131_02465"
     CDS             complement(326..700)
                     /locus_tag="PK131_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HPD45014.1"
                     /translation="MLYNQNITDSISLNLTKQYLGKEVDIVINRAVGSTHPKWNFIYE
                     CNYGYIKGVLAPDGEELDAYLLKVNTPVETFHGQVIAIIHRLKDDDDKLVVVPTGQTI
                     TDEEIEKIVAFQEKWFEHEIIR"
     gene            975..2009
                     /locus_tag="PK131_02470"
     CDS             975..2009
                     /locus_tag="PK131_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45015.1"
                     /translation="MGRKIGVWLGLLITGVWLGQKFIPYLGFFPYKEELHRLPPAVLQ
                     PLGNFDGIHYLLIARQGYQQYEQAFFPLYPNLIKVMGNLPLVNGVLISVISFILGLFI
                     WQKYFQKIGLPADKILWFWILFPTAFFFSTVYPESLFFLLMGLTLWLTAEKKWLLAIL
                     TGFLAAQTKLIGLALVIPIFWQNRKQWWIGLGPVAGFLSYCLYLWRTVGDPLAFYHAQ
                     PMFGANRATRLIFLPQVIWRYLKIFALAGHDFKYFIAILELVTLIGCLYLLCRKIVWR
                     YWGLALFSLIVIILPTLTGTLSSLPRYALLAPAIFISLADRNNLWTRIVFGFLFVVLT
                     AFFTQGYFVS"
     gene            complement(1984..>2494)
                     /locus_tag="PK131_02475"
     CDS             complement(1984..>2494)
                     /locus_tag="PK131_02475"
                     /inference="COORDINATES: protein motif:HMM:NF022889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HPD45016.1"
                     /translation="DKIKFVFPLVFFKVIYAAVVGLIIDLLFPHNQLIPRSSVKTESA
                     CCGQHQSFSFHPLTHALKLTGYIFGLNLILGWIFELFHPAVLLNPFLAAIIGLIPNCA
                     ASVFLTEAFLKNVISFGSLFAGLCASAGLGLFLLFKESDSYHRSFVILALLYIFSVLG
                     GFMIQSILG"
BASE COUNT          767 a          475 c          423 g          829 t
ORIGIN      
        1 atatggcacc tttcttccaa attaaataat tcgaatttgt ctaagatcgt tgagcgcatt
       61 ggaattattt aatttggaag aaaggtgcca tatgaagaaa taattttatc cgacattttt
      121 tcctcatttt ctagttttaa tacacaggta tcaagagttt ctccaccaaa gtcaaaccca
      181 aaatgaacct ctttaaatct ggcaagtttg actgccgttt caagtccatt ttaagcttat
      241 cctcagttat gttattggca aatctaatag gatgtcctaa aaacccacac attcactaac
      301 acagaggtat tactagtacc aaaaattacc ttattatttc atgttcaaac catttctctt
      361 gaaaagcaac tattttttct atctcttcat cggttattgt ttgtcctgtt ggtaccacca
      421 ctaatttatc gtcatcatct tttagacgat gaataattgc aataacttgg ccatgaaatg
      481 tctcaaccgg agtatttact ttaagtaaat atgcatctaa ttcctccccg tccggagcta
      541 atactccttt tatatatcca taattgcact catagataaa attccatttt ggatgggtgc
      601 tgccaacggc tctattaatt actatatcga cttcttttcc aagatattgt tttgttaaat
      661 ttaatgaaat tgagtctgtt atgttttgat tatataacac cttagaatta ttttaattct
      721 tgtttgtatt cactaataat ttttctgagc atttttgtcg cgcttatatg ttgaagacgg
      781 gcttgcttat tcaaaaacgt cagttcatta ttggtaagca caagacggga ttaaaacggt
      841 caaggtttat atcaactcat ccagcggtaa gctaataacc tttcttttat cctcaacggt
      901 caaaaattaa aaggcagtta ctgattaata aaattcaaag aaccaaatct ctggtttatc
      961 gttaaaatat aaacatgggg aggaagatag gagtttggct aggcttacta ataaccgggg
     1021 tatggctggg gcaaaaattc attccttatt taggtttctt tccttataaa gaggaacttc
     1081 atcgtttacc tccggctgtt cttcaacctc tgggcaactt cgacggtatt cattatctgt
     1141 taattgcccg gcagggctac caacaatatg aacaagcttt ttttccatta tacccaaatt
     1201 taatcaaagt catgggtaat ttaccattag taaatggtgt tttaatttca gtaatttcat
     1261 tcattttagg attatttatt tggcaaaaat actttcaaaa aatcggtctg ccggctgaca
     1321 aaattctatg gttttggatt ttatttccaa cagctttctt tttcagcact gtttatcccg
     1381 aaagtttatt ttttctctta atgggtttaa ccctgtggtt aacagccgaa aaaaaatggc
     1441 tgctggcaat tttaaccggc ttcttagccg ctcaaactaa attaattggt ctggcattag
     1501 ttattccaat tttttggcaa aatcggaaac aatggtggat tggcttggga ccagtagctg
     1561 gctttttaag ttactgtctc tacctctggc ggactgtcgg tgatccgctg gctttttacc
     1621 atgctcagcc gatgtttggg gctaatcggg ctacccgatt aatttttctg ccgcaggtta
     1681 tttggcgcta cctcaaaatt tttgctttag ccggccacga ttttaagtat tttatcgcca
     1741 ttcttgaatt agtcaccttg attggctgcc tatatctgct ttgtcgtaaa atagtctgga
     1801 gatattgggg attagccctt ttttccttaa tcgtaattat tctgccgact ctaaccggca
     1861 ctctttcctc gttaccccgt tatgctttat tggctccggc gatttttatc agtttagctg
     1921 atagaaacaa tctctggacc agaatagtct ttggcttttt atttgtcgtt cttacggctt
     1981 tttttaccca aggatacttt gtatcataaa accaccaaga acactaaaaa tatagagcaa
     2041 agctaaaata acaaacgaac ggtggtagct atcactttct ttaaacaaca aaaaaagacc
     2101 aagaccggca ctagcgcaaa gaccagcaaa aagagaacca aaggaaatga cattttttaa
     2161 aaaagcctcg gtcaaaaaaa ctgaagcggc gcaattagga attaggccga tgattgccgc
     2221 caaaaaagga tttaataaga ccgcaggatg aaataattca aaaatccagc ccaaaattaa
     2281 atttaaacca aaaatataac ctgtcaattt caaggcatga gtcaagggat gaaaagaaaa
     2341 agactgatgt tggccacagc aggcggattc agtttttacc gatgatctgg gaattagctg
     2401 attatgagga aataataaat caattatcaa accaaccaca gcggcataaa tcaccttaaa
     2461 gaaaactaaa ggaaaaacaa atttaatttt atca
//
LOCUS       DAOOIE010000081         2433 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_33959_length_2433_cov_3.317073, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000081 DAOOIE010000000
VERSION     DAOOIE010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33959_length_2433_cov_3.317073"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..787
                     /locus_tag="PK131_02480"
     CDS             <1..787
                     /locus_tag="PK131_02480"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPD45017.1"
                     /translation="LSFFQEHKPIIREKSFENYLRNYLKTTYQLSPTHLNSYLNCPLC
                     FYYRTILRVPLAKSKLASYGSAVHYALTDLYRNSLSLEKFLEIFENFLRKENLNSADF
                     KENLAKGIKELSEYYHHYQDSFQGKFLLEYDFKKLNVYLDNVPITGRIDKLELNGREA
                     VAVDFKTGSFKNNRHKEDYQRQILFYKLLGDHCPQFPYQIKSGILDYIGECRRQPVAI
                     NPESLEKLVDLIKEVYCKILKLEFNQIGQDCEDKDHFHQFQKV"
     gene            747..1196
                     /locus_tag="PK131_02485"
     CDS             747..1196
                     /locus_tag="PK131_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPD45018.1"
                     /translation="MKIKITSINFKRFDKTLPPPEFKTKGAVAADVSSRVDLDFAPGE
                     IKLVPLNVAMEIPEGYCMFLISRSSTYKLGIRSVNGIGVFDNDFCGDNDEFHFIAQNF
                     TDHPVHLEKGMRIAQILLIKKENFSLKEVDNLGHHPTRGGIGSTGVK"
     gene            1204..2052
                     /locus_tag="PK131_02490"
     CDS             1204..2052
                     /locus_tag="PK131_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45019.1"
                     /translation="MGEIHSEEANKKSTIAKKVAAGLATAVTITSLAACDYSPSNKGT
                     IPAQKSEGIADRVRDIERERMRDEERRKIIDSGNPLIDQICQDTIDRKTEAAGVVQVE
                     MIKTLASQDYLKPGEICFLDCGESYLEGKIKMVMVNTQTGEVFPINQVLDYQAVNDQP
                     LFVYSKINRESGNPQAVYQIGFLNDGEHDVKNLGNIGENSILSLVREKTKAGLTGSYN
                     EINLGVIKATAEVGNYTYTQDLPGNIISYHFLSGGEIRAESLKGNDDEAGLALNLQLK
                     INNLSK"
     gene            2060..>2433
                     /locus_tag="PK131_02495"
     CDS             2060..>2433
                     /locus_tag="PK131_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45020.1"
                     /translation="MIPVYLILIAILGALIFLIFRSKKDNKEELSQKLKEIFPEVLKN
                     ANEQLITLADQKIGTDLANKKAAIEDLVKQVKVELEKNQKRLETAESARIGNFESLKT
                     ALGEYKKITQELAVSTEGLKRA"
BASE COUNT          807 a          389 c          513 g          724 t
ORIGIN      
        1 tctgtcattt tttcaagagc ataaaccaat aattcgggag aaaagttttg aaaactatct
       61 tcgaaattat ctaaaaacca cttatcaatt aagcccaacc catcttaatt cctatcttaa
      121 ctgtccactt tgtttttatt atcgaacgat tttaagggtg cctttagcca aaagtaaact
      181 tgcctcttac ggttcggcag ttcactatgc tctgactgat ctatatcgta attcgctatc
      241 tttagaaaaa tttttggaaa tttttgaaaa ttttttgcgg aaagaaaatt taaattcagc
      301 cgattttaag gaaaatttag ctaaggggat aaaagaattg agtgaatatt accatcatta
      361 tcaggatagt tttcagggaa aatttttgtt ggagtatgac tttaaaaaat tgaatgttta
      421 tttggataat gtgccaatta ccggcaggat tgataaatta gaattaaacg gacgggaagc
      481 agtggcggtt gattttaaaa ccggttcttt taaaaataat cgtcataagg aggattacca
      541 gcgacagatt ttgttttaca aactgttggg tgaccattgt cctcaatttc cttatcaaat
      601 aaaatcaggc atccttgatt atattggtga atgccgccgc cagccggtag caattaatcc
      661 cgaatcattg gagaaattag tagatttaat caaggaagtt tattgtaaga ttttaaaatt
      721 agaatttaat caaattggcc aagattgtga agataaagat cacttccatc aatttcaaaa
      781 ggtttgataa aaccctgccg ccgccggagt ttaaaactaa aggagcggta gcggctgatg
      841 tttcctctcg ggtggatttg gattttgctc ccggggaaat taagctggtg ccgttgaatg
      901 tcgctatgga aattcccgaa ggctactgta tgtttttaat ttcccgcagt tcaacttaca
      961 aactaggaat tcgctcggtt aacggcattg gcgtttttga caacgatttt tgcggtgata
     1021 acgatgagtt tcattttatt gctcaaaatt ttaccgatca tccagtccat ctagaaaagg
     1081 gaatgcggat tgcccaaatt ttattaataa aaaaagaaaa tttttctcta aaagaggtgg
     1141 ataatcttgg ccatcacccc actcgtggcg gaattggctc aactggagta aaataatata
     1201 tctatgggag aaattcattc tgaggaagct aataaaaaat caacaatagc taaaaaagtg
     1261 gcggccggat tagctacggc ggtaacaata accagcctag cggcctgcga ttattccccg
     1321 tcaaacaaag gaacaattcc agcccaaaag tcggaaggaa tagccgatcg ggtcagggat
     1381 atcgagagag aaagaatgcg agatgaagag cggagaaaaa ttatcgacag cggtaatcct
     1441 ttaatcgatc aaatttgtca ggatactatt gaccgtaaaa cagaggcagc cggggtagtt
     1501 caagtagaaa tgattaaaac tttagccagt caggattacc ttaaacccgg tgaaatttgt
     1561 tttcttgatt gcggagagag ttatcttgaa ggtaaaatta aaatggtgat ggttaatact
     1621 caaactggtg aggtttttcc gattaatcag gttttagatt atcaggcggt taacgaccaa
     1681 ccactttttg tttacagtaa aattaaccgg gagtcaggta atccacaagc cgtttatcaa
     1741 atcgggtttt taaacgatgg tgaacatgat gttaaaaatc ttggtaatat tggtgaaaat
     1801 agtattttat ctttagtcag agaaaaaacc aaggctggtc tgaccggcag ttataatgaa
     1861 attaatttgg gtgttatcaa agcaacagcc gaagtcggaa attatactta tacccaggat
     1921 ttaccgggga atattatcag ctatcatttt ttaagcggtg gggaaatcag ggccgaaagt
     1981 ttgaagggta atgatgatga ggccggttta gctttaaatt tgcaactgaa aatcaataat
     2041 ctgtcaaaat aaaatataaa tgattccggt ctatttgatt cttattgcca ttttaggcgc
     2101 tttgattttt cttatcttta ggtcaaaaaa ggataataaa gaggaattaa gtcaaaaatt
     2161 aaaggagatt tttcctgagg ttcttaagaa cgctaacgaa cagctaatca ctctggccga
     2221 tcagaaaatc ggcaccgatt tggctaataa aaaagccgct atcgaggatt tggtcaagca
     2281 ggttaaggtt gaattggaaa aaaatcaaaa aagactggaa acagctgaga gtgctcggat
     2341 tggtaatttt gaatctctaa aaactgctct aggtgagtat aaaaagataa cccaggagtt
     2401 agcggtcagt actgaaggtt taaaacgagc ctt
//
LOCUS       DAOOIE010000082         2417 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_34270_length_2417_cov_3.815834, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000082 DAOOIE010000000
VERSION     DAOOIE010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2417
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34270_length_2417_cov_3.815834"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="PK131_02500"
     CDS             complement(<1..635)
                     /locus_tag="PK131_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45021.1"
                     /translation="MNQKAIYRNFWLIIGITFVGVIFNLPKRPNFSLKIGNFDFSKRM
                     DLHFGLDLAGGASLIFDIDTTQTKKEDLTNALESLKSNIERRVNLFGVSEATVQLSTS
                     DGQNRLTVELPGVEDVDQAVALIGKTAQLNFKGETELASEATASATIYDVFKEDTGIN
                     GSHLVRSQVVVNPNTGQPEVGLEFNNEGAKLFEAATKKFLNKRLAIFLDDV"
     gene            complement(654..1244)
                     /locus_tag="PK131_02505"
     CDS             complement(654..1244)
                     /locus_tag="PK131_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45022.1"
                     /translation="MKANNQLRRVLVSQTRIKLIDILFYQPREIFYVRELVRLTDEEI
                     NSVRRELANLLEADLIFQDKRANRIYYGANSQSFYFYDLLLLAHKNSGLGGNFFAWKT
                     KIGRLKYVLMSFDFAMGSTYSSDIIDLVIVGDVSYKEVEKLIKEEEDQRNREINYMIM
                     DNNEFRLRRNRRDQFIIDFFLRSPLVIFGNLNYLDS"
     gene            complement(1234..>2417)
                     /locus_tag="PK131_02510"
     CDS             complement(1234..>2417)
                     /locus_tag="PK131_02510"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HPD45023.1"
                     /translation="LIWGKLLPITSKAIISPVGHEMALPSGSQLPGVLNFEGPRTEVC
                     PINGQKFTKEEKEIWLQRRPLIVMIENHVDARPQSGLSNADVVYEAVAEGGITRFMGV
                     FYCNIVKGAPNKYDVGPVRSARSYFVDLASEYADYPLYVHVGGANCSAVKDPVTGQQV
                     GSCTTNRKAMAIEQIADYGWNNAGTWSDLSQFALPYKVCRREPERIGPNTAMEHTMYC
                     STKELWNIAAERGLTNETKAKQTNWDKNFQPWKFAAEDNPSSTAKKIQFNFWSGYNDY
                     AVTWDYDPSANVYRRSNGDQIHLDFNTKKQLEAKNVVIQFVKESRSIDEHLHNLYEVV
                     GSGKGILFQNGDKIDITWSKAKRTSRTIFKDSTGREIEFVPGQIWIEILPLNNAVSYE
                     G"
BASE COUNT          783 a          539 c          373 g          722 t
ORIGIN      
        1 aaaacatcat caagaaaaat ggctagccgc ttatttaaaa actttttggt agcagcttca
       61 aataatttag ccccctcatt attaaattcc aaacccacct ctggttgacc agtattggga
      121 ttaaccacaa cttgagaccg aaccaaatgg gaaccgttaa ttcccgtatc ttccttaaaa
      181 acatcataaa tagtcgctga cgcagttgcc tccgatgcta attctgtctc acccttaaaa
      241 ttcaactgag ccgttttacc aattaaagca accgcctgat ccacatcctc caccccgggt
      301 aattccaccg tcagccggtt ttgaccatca ctagtagaaa gttgaactgt agcttcagaa
      361 acaccaaaaa gatttactcg tctttcgata ttactcttta atgattccaa agcattggtt
      421 aaatcctctt ttttagtttg agttgtatca atgtcaaaaa tgagagaagc accaccggcc
      481 aaatcgagac caaaatgtaa atccattctc ttactgaagt caaaattacc tattttaagg
      541 ctaaaattgg ggcgtttagg taaattaaaa attactccga caaaagttat gccgattatt
      601 agccaaaaat tacgataaat cgctttttga ttcatgcctt attctacctc aatttaactg
      661 tctaaataat taagattacc aaaaattacc aatggagaac gaaggaaaaa atcaataatg
      721 aactggtctc ggcgatttcg tcggagtcga aattcattat tgtccattat catataattt
      781 atctctcgat tccgttgatc ctcttcttct ttaattaatt tttccacttc tttataggaa
      841 acatcaccaa caattaccaa atcaattata tccgacgaat aggtagaacc catagcaaaa
      901 tcaaaactca ttaaaacata ttttaatcgg ccgattttgg ttttccaagc aaaaaaattg
      961 cctcctaaac cagaattttt atgagctaat aaaagcaggt cataaaaata aaatgactga
     1021 gaattggcac catagtaaat ccgattagct cgtttatcct gaaaaattaa atcggcttca
     1081 agtaaattag ccaattcccg acgaacggaa ttaatttctt cgtcagtcag acgaactaac
     1141 tcacgaacat aaaaaatctc ccgtggctga taaaaaagaa tatcaatcag tttaatcctt
     1201 gtttgagaaa ctaaaactct tctcagctga ttgttagcct tcataagaaa ctgcgttgtt
     1261 gaggggcaga atttctatcc aaatctggcc tggaacaaat tctatttccc tgccagtaga
     1321 atctttaaaa atagttcggc ttgttcgctt agctttggac caagtaatat cgattttatc
     1381 tccattttga aataagatcc ccttaccact gccaacaact tcatagagat tatggagatg
     1441 ctcatcaatc gaccgagatt ctttaacaaa ttgaattacg acattcttcg cttctagctg
     1501 ttttttagta ttaaaatcaa gatgaatttg gtcaccattg ctacgtcgat agacattagc
     1561 cgacggatcg taatcccatg tcaccgcata atcattatag ccgctccaaa aattaaattg
     1621 gattttttta gccgttgaac tcggattatc ttcagccgca aatttccatg gttgaaaatt
     1681 tttatcccaa ttggtttgtt tagctttagt ttcattagtt aaaccccgtt cagccgcaat
     1741 attccataat tctttagtgg aacaatacat ggtatgttcc atggctgtat taggaccaat
     1801 ccgttccggc tcacggcgac aaactttata tggcaaagca aactgagaca aatcactcca
     1861 agtaccggca ttattccaac cataatcagc tatttgctca attgccattg ctttccgatt
     1921 ggttgtacat gaacctacct gctgacctgt aaccggatct ttaaccgctg aacaattcgc
     1981 tccaccaaca tggacataca aaggataatc cgcatattcc gaagccaaat caacgaaata
     2041 actccgagct gaccgaaccg gaccaacatc atacttattt ggtgctccct taactatatt
     2101 acagtagaaa acacccataa accgggtaat accaccctca gccaccgctt cataaacaac
     2161 atcggcattg cttaaaccgg attggggccg agcatcaaca tgattttcaa tcattactat
     2221 taaaggccga cgttgcaacc aaatttcttt ttcttcttta gtaaattttt gcccgttaat
     2281 aggacacact tctgttctcg gtccctcaaa gttaagaact ccgggtaatt gactcccgga
     2341 gggaagagcc atttcatgac cgacaggaga aataattgct tttgaagtaa ttggtaataa
     2401 tttaccccaa attaaaa
//
LOCUS       DAOOIE010000083         2410 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_34420_length_2410_cov_2.898514, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000083 DAOOIE010000000
VERSION     DAOOIE010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34420_length_2410_cov_2.898514"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            202..387
                     /locus_tag="PK131_02515"
     CDS             202..387
                     /locus_tag="PK131_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45024.1"
                     /translation="MKETENKTIIMEDVNGTLKVIEKGTDYFNDEFWPDHILNSPDGK
                     RLLRMADYLGTDCDDSI"
     gene            391..1413
                     /locus_tag="PK131_02520"
     CDS             391..1413
                     /locus_tag="PK131_02520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPD45025.1"
                     /translation="MKKTTQKPTKIIVGLLIVGLFLVIYSWRPKSKEQYYQITPKRQS
                     IQSNLILAGTINAAAKTSLHFQTAGQLAWVGVKVGDRVKKYQAIASLDKTQLKKNLEQ
                     QFLNYKNGLSTFNDTQDTYKTNKENSTLTEAMKRILERSQNALDSAVINYELADLTIK
                     YATIVSPIAGIVTDVPNALPGTNITPLDTAFTVVDPATIYFRSEIDQENVNQIKIGQA
                     AKITLDSSPDDSIESQINFISFTPVAGQTSTVYEVRFDLPINNQDLTYRLGMTGDAEI
                     ITQRADNVLTVPIEAVFEKNNKKMVLVKTGSQQIEKEVVTGIESETDIEIKSGLSEYD
                     QVLIKK"
     gene            1388..2083
                     /locus_tag="PK131_02525"
     CDS             1388..2083
                     /locus_tag="PK131_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPD45026.1"
                     /translation="MIKSSLKNNQPLLELRNVYKTYILGETKFDALKGVSCQINPGEF
                     VAITGPSGSGKSTMMHLIGLLDTPTSGDIFLEGKNVSKLSEIQLAQLRNTTLGFIFQQ
                     FNLLPKTPAIENVILPLLYSDIPQSKHYALGVEMLKKVDLLDKLNNTPAQLSGGQQQR
                     VAIARALVNNPKIILADEPTGNLDSKSGREIISILHYLNEKEGRTIIMVTHDLDLAKE
                     TQRQINLKDGQIC"
     gene            2077..>2410
                     /locus_tag="PK131_02530"
     CDS             2077..>2410
                     /locus_tag="PK131_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007392909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD45027.1"
                     /translation="MLNIKPDLNELVKSATHSILKNKSRTFLTSLGIIIGVTSVILLI
                     SIGNGLKSYITDQFESLGSNTIYIVPGKILSNNGNLKPGEATGILNITFTEKDVQNLQ
                     RELPVAAVL"
BASE COUNT          812 a          499 c          422 g          677 t
ORIGIN      
        1 cattttagat cgggaaaaag caatcaaaaa agctctcgaa tcagcccaaa agggcgacgt
       61 cattttgatt accggcaagg gggctgagca atcgatcact atcggcggtc aaaaataccc
      121 ttgggatgat cgaaaagtag tcaaaagatt acttaatgtt gtataatact caaaccaatt
      181 ttagttatta actctaaact tatgaaagaa actgaaaata aaacaattat tatggaggat
      241 gttaatggca ctctgaaagt aattgaaaag gggactgatt attttaatga cgaattctgg
      301 cccgatcata ttttaaactc tcccgatggt aaaaggttgt taagaatggc tgattatcta
      361 ggaactgatt gcgacgattc tatttaaata atgaaaaaaa ctacccaaaa accaactaaa
      421 attattgttg gccttttgat tgtcggcctt tttctggtga tctattcttg gcgacctaaa
      481 tccaaggagc aatattatca aatcaccccc aaacgacaat cgattcagtc aaatttaatc
      541 ctagccggaa ctatcaacgc cgccgctaaa accagtcttc attttcaaac tgctggtcaa
      601 ttggcttggg taggggttaa agttggtgat agagtaaaaa aataccaagc aattgcctcc
      661 ctcgacaaaa cccaacttaa aaagaattta gaacaacaat tccttaatta taaaaatggc
      721 ttaagcactt ttaatgatac ccaagatact tacaaaacta acaaagaaaa ctccactcta
      781 accgaagcaa tgaaacgcat tttggaacgg tctcaaaatg ccttagacag cgccgtcatt
      841 aactatgaat tagccgattt aacaattaaa tatgccacga tagtttctcc aattgccggt
      901 atagttactg atgtccctaa tgctctccca ggaactaata tcaccccact cgataccgct
      961 ttcactgttg tcgatccggc cactatttat ttcaggtcag aaattgatca agaaaatgtc
     1021 aatcaaatta aaattgggca agcggccaaa attacccttg atagctctcc agatgattct
     1081 attgaatccc aaattaattt tatttctttc actccggttg ccggtcaaac ttcaaccgtc
     1141 tatgaggtca gatttgattt accgattaac aatcaagatt taacctaccg cctaggaatg
     1201 actggtgatg ccgaaattat tacccaaaga gccgataatg ttctaacggt tccgattgag
     1261 gccgtttttg aaaaaaataa taaaaaaatg gttttggtta aaactggcag tcaacaaatt
     1321 gaaaaagaag tggtaaccgg aattgaatcg gagaccgata ttgaaattaa atccggccta
     1381 tctgaatatg atcaagtcct cattaaaaaa taatcagccc cttttagaat taaggaacgt
     1441 ctataaaacc tacattttgg gggagactaa atttgacgcc ttaaaagggg taagctgcca
     1501 aattaatcca ggtgaattcg tcgctattac cggtccttcc ggctccggta aatcaactat
     1561 gatgcatctg attggtttat tagatacccc aacttcaggc gatattttcc ttgagggaaa
     1621 aaacgtttct aaactatccg aaattcagtt ggcccagttg cgcaatacca cccttggctt
     1681 tatttttcaa caattcaacc tgctccctaa aactccggca attgaaaacg ttattttacc
     1741 cctgctttac tctgatattc ctcaatccaa acattatgct ctcggggtag aaatgttaaa
     1801 aaaggttgat ttattagata aactcaataa tacgccggcc cagctctccg gcggccagca
     1861 gcaacgggtg gccatcgccc gggctttagt taataatcct aaaataattc tggccgatga
     1921 acccaccggt aatttagata gcaaatccgg ccgggaaatt ataagcatcc tccattatct
     1981 gaacgaaaaa gaggggagaa ctatcattat ggttacccat gatttagatt tagccaaaga
     2041 aacacagcgg caaattaacc taaaggacgg ccaaatatgt tgaacattaa acctgatctc
     2101 aatgaactag ttaaatcagc tactcattcg attctaaaaa acaaaagtcg aacttttcta
     2161 acatctcttg ggattattat cggcgttact tccgttattc tacttatttc tatcggcaat
     2221 ggtctcaaaa gttatatcac cgatcaattt gaaagccttg gttcaaatac tatttacatt
     2281 gttcccggca aaatcttgag caacaatggt aatttaaaac caggagaagc aacagggatt
     2341 cttaatatta ccttcactga aaaagacgtt caaaatcttc aaagggaatt accggtagca
     2401 gctgttctac
//
LOCUS       DAOOIE010000084         2299 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_36814_length_2299_cov_2.601604, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000084 DAOOIE010000000
VERSION     DAOOIE010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36814_length_2299_cov_2.601604"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PK131_02535"
     CDS             complement(<1..508)
                     /locus_tag="PK131_02535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD45028.1"
                     /translation="MAAVKAILILSPFFPPNIGGVETHLADLCLELARKNYKVYVHTY
                     SPLTTSVKYQKYEKSKNLTIYRYPWLGKNLFHRLEKYPLLDFLYLTPYLFLRTFFWML
                     LNSHKINIIHSQGFNAAFAGYCLSLIFHKKHLISTHAVYDNISTSTPLRFILNHSDHV
                     LCLSHASQK"
     gene            complement(496..1500)
                     /locus_tag="PK131_02540"
     CDS             complement(496..1500)
                     /locus_tag="PK131_02540"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPD45029.1"
                     /translation="MISVSFIIPTLNAGRVLGKCLASIKKQRTKYHYEIIIIDGGSDD
                     KTLKIAKKNQAKIISNHLKTAEAGKAAGVKIARGKYLAFIDSDNILPSPNWLNQMLQP
                     FSEKEIIGSEPIKFTYRKNAGLIERYSALIGANDPYALITGVYDRFSYLTQKWTGLNL
                     STTDKRKYLKIKLEKNKLLPTIGANGTIFRRQLFQNFRSDYLFDIDFLTSQLKHRHYL
                     YFAKVKTGIIHTYCENSIKKFIRKQNRRVVDYFTYRRQRHFNWDQTNKWGTIKFVFYT
                     LTLLPMIYHLILGYSRQPDRAWFFHPLACLITLYCYTINYLRYQLGLIKPLNRNQWRQ
                     "
     gene            complement(1487..>2299)
                     /locus_tag="PK131_02545"
     CDS             complement(1487..>2299)
                     /locus_tag="PK131_02545"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPD45030.1"
                     /translation="GRDCWWGRCTFCSWAALFPGNHYQVVSPLKAIEEIQLLQHLGVK
                     EVFDDSGTFPVGEWLEQFCRLMIKSGLNKKIQLGANMRFGALSQTEFNLMKKAGFRFL
                     LFGLESANQTTLNKINKNIKVDNVIPTLTMAQKAGISSHITIMVGYPWENKKDLNKTL
                     AFCRSIFRQGLVASMQATIIVPYPGTPLFNYCRKNNLLTTSNWNKYDMGQPVIKTKVS
                     ADYLKQSVRDLFKGIITPRFIFNQITSLKSWADFQHLLFYTIKYFQKLNDFR"
BASE COUNT          713 a          364 c          515 g          707 t
ORIGIN      
        1 gtttttggga agcgtgggag aggcaaagaa catggtcgga atgattgagg atgaaacgca
       61 aaggggtgga agtggaaatg ttgtcataaa cggcgtgggt actgatgaga tgttttttgt
      121 ggaaaataag ggaaagacag tagccagcaa aagcggcgtt aaagccttgg gaatgaataa
      181 tgttaatttt gtggctattg agtaacatcc agaaaaaagt gcggagaaaa agataagggg
      241 ttaaatataa aaagtcgagc agtggatatt tttccagccg gtggaaaaga ttcttgccca
      301 accatggata gcgataaata gttaaatttt ttgatttttc atatttttga tatttaaccg
      361 aggtagttaa gggcgaataa gtatggacat aaactttata gttttttcga gctaattcta
      421 ggcaaaggtc agctaaatgg gtttctaccc cgccgatgtt gggtggaaaa aaggggctaa
      481 gaattagaat tgcttttact gccgccattg atttctgttt aaaggtttaa ttaaaccaag
      541 ttgataccga aggtaattga tagtgtagca gtaaagggta attaaacaag ctagggggtg
      601 aaaaaaccat gcccggtcag gttgacggct ataacctaaa attaggtgat aaatcatagg
      661 aagaagagtt aaggtataga aaacaaattt gatagttccc catttattag tttgatccca
      721 attaaagtgg cgttgacgcc gataggtaaa ataatcaact actcggcggt tttgtttgcg
      781 gataaacttt ttaattgaat tttcacaata agtatggata ataccggttt tgactttggc
      841 aaaataaagg taatgtcgat gttttaattg agatgttaaa aaatcaatgt cgaataaata
      901 atccgaccgg aagttttgga aaagttgccg gcggaaaata gtcccgttag ccccaatagt
      961 tggcaggagt ttattttttt ccaattttat tttcaaatat tttcttttat cagttgtcga
     1021 cagatttagg ccagtccatt tttgggtaag gtaactgaag cgatcataga caccggtaat
     1081 tagggcataa ggatcattag caccgatgag agcgctatat ctttcgatta aaccagcgtt
     1141 tttacgataa gtaaatttta tcggttcact gccgatgatt tctttttcgg aaaaaggctg
     1201 aagcatttgg ttaagccaat tgggactagg aagaatatta tcagagtcaa tgaaggctaa
     1261 gtattttccc cgggcgattt tgaccccggc ggccttgccg gcttcagccg tcttgagatg
     1321 attggagata attttagctt ggtttttttt ggctatttta agggtcttat cgtcactgcc
     1381 gccatcgatg ataataattt cataatgata ttttgttctt tgttttttaa tcgaagccag
     1441 acattttccc aaaaccctgc cggcgttgag agtgggaatg ataaagctaa cggaaatcat
     1501 ttagtttttg gaaatattta atagtgtaaa aaagtaagtg ctgaaaatca gcccatgatt
     1561 ttaaagaagt aatttggtta aagataaatc ggggggtaat aattcccttg aataaatctc
     1621 ggaccgactg tttaaggtaa tcggcagaga ctttagtttt gataaccggc tgacccatgt
     1681 catatttgtt ccaattactg gtggttagca gattattttt ccggcagtaa ttaaagagcg
     1741 gtgtcccggg ataaggaaca ataatagtgg cttgcatact ggcgaccaat ccctggcgaa
     1801 aaatgctacg acaaaaagct agggttttat tcaaatcttt tttattttcc caagggtaac
     1861 caaccataat agtgatatgg ctgctaattc cggctttttg agccatagtt aaagtgggta
     1921 taacattatc aactttaata tttttattga ttttatttag agttgtttga ttggccgatt
     1981 ccagaccgaa aaggagaaag cggaatccag cctttttcat taaattaaat tcagtctgac
     2041 ttaaagcgcc aaaacgcata ttagctccca attgaatttt tttgtttaag ccggacttaa
     2101 tcattaaccg gcagaattgc tctaaccact cccccactgg aaaagtgcct gaatcatcaa
     2161 agacctcttt tactccaagg tgttgaagta attgaatttc ttcgattgcc tttaatggtg
     2221 aaactacttg gtaatgattg ccgggaaaaa gggctgccca agaacaaaag gtgcatcttc
     2281 cccaccaaca atcgcggcc
//
LOCUS       DAOOIE010000085         2279 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_37232_length_2279_cov_3.563849, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000085 DAOOIE010000000
VERSION     DAOOIE010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37232_length_2279_cov_3.563849"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            199..1407
                     /locus_tag="PK131_02550"
     CDS             199..1407
                     /locus_tag="PK131_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HPD45031.1"
                     /translation="MRYSQLFGRTTKNIAADADSINAKLLTQAGFINKELAGVYSYLP
                     LGLQVLTKIQNIVREEMNQLGAQEILLPVLSSCQKWQKTGRDKLTVLYRLGQLVLNPT
                     HEEVVTPLVQQYVFSYRDLPLAVYQIQEKFRHEPRAKSGLLRNLEFNMKDLYSFHADE
                     KDLDKFYNQVIETYRRIFNRLNLGEITFLTYASGGSFSKYSHEFQTICPTGEDLIYLC
                     DQCQVAVNKELIEEQKTCPKCNSANLVEKKAIEVANIFKLGTKFSAAFGFNYTNEQGE
                     LRPVLMGCYGLGPSRLMGTLVEVFHDDRGIIWPEVAAPYPIHLVGLDPKAEEIYHKLG
                     ENNVLFDDRKEVTPGVKLTDADLIGLPWRWLVSSKSLQAGGIEIKRRNSNKIKIIPVD
                     DAILKYGQTR"
     gene            1391..>2279
                     /locus_tag="PK131_02555"
     CDS             1391..>2279
                     /locus_tag="PK131_02555"
                     /inference="COORDINATES: protein motif:HMM:NF036827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HPD45032.1"
                     /translation="MAKRGRKRKRRFFSLFKIKRETFQTILFLFFFMAAIITVFSFLE
                     IGPIPTKISLTLQNYFGFLAVFIPFLFLLTAFLFVKVKFPLKEPNIFLGFVIMFIALI
                     SLFRAGIVGNYCRDLLINSFGELISSIVYFICLIAGFVILFDTNVAQLVKFCVKIFKA
                     IKKYTVGSGVKSTVRREKIVPRLGSSLPPKDETKDEKQVKIEDYVINAPFKSPVPVGS
                     NQIWQYPPITLFDNSLGAPADRGNVGKNAKTIEETLDSFGITAKVVEINDSPSVTQYA
                     LEVALGTKLAKIVSLSNDLA"
BASE COUNT          669 a          380 c          479 g          751 t
ORIGIN      
        1 aattgcggtt ctaaattgag aaaagagttg taattttacc ccgacgggta taatttagat
       61 tatgcctgat aaaaatcttc aaaatctaat tcaatcgttc cgtcaagaag ggcgaataaa
      121 aatgaaaaat tttggccaag gaaaaaagag tgatctgttc cgggtcagaa aagtaatttg
      181 attgggctat aatagtttat gcgttattct caactttttg gccggacaac gaaaaatatc
      241 gccgctgatg ccgattcgat taatgccaag cttttgactc aagccgggtt tattaataag
      301 gaattagccg gtgtttacag ctatttgcct cttggccttc aggtattgac taaaattcaa
      361 aatattgtcc gggaggaaat gaatcaattg ggggctcagg agattttact gccggtttta
      421 tcctcttgtc agaagtggca gaaaaccggc cgcgataaat tgactgttct ttatcggctg
      481 ggtcagctgg ttttaaatcc aacccatgag gaagttgtta cccctttggt tcagcaatac
      541 gttttttcct atcgggattt acctttggct gtttaccaaa ttcaagaaaa atttaggcat
      601 gaacctcggg ctaaatccgg tttattacgg aatttggaat ttaatatgaa agatctctac
      661 tcttttcatg ccgacgaaaa agacttggac aagttttata atcaggtgat tgagacatac
      721 cgaagaattt ttaatcgcct taatttaggc gaaattactt ttttaactta tgcctccggc
      781 ggtagttttt ctaaatattc tcatgaattt cagactattt gcccaaccgg tgaagattta
      841 atttatcttt gtgatcaatg ccaagtggcg gtaaataagg aattgattga agagcaaaaa
      901 acctgcccta aatgtaattc ggccaattta gttgagaaaa aggcgatcga agtcgccaat
      961 atttttaagc tgggaactaa attcagtgct gccttcggtt ttaattatac caatgaacag
     1021 ggagagttaa gaccggtttt aatgggttgt tatggtttgg gaccaagtcg tctaatggga
     1081 accttggtgg aagtttttca tgatgaccgg gggattattt ggccggaagt agcagcgcct
     1141 tatccgattc atttagtcgg tcttgatccc aaagccgaag aaatttacca taaattgggt
     1201 gagaacaatg ttctttttga tgatcggaag gaagttactc cgggagttaa gttgaccgat
     1261 gcggatttaa tcggtttgcc ctggcgctgg cttgtttcat ccaaaagtct tcaagccggc
     1321 ggaatagaaa taaaacgtcg aaatagcaat aaaatcaaga taattccggt tgatgatgct
     1381 atactgaaat atggccaaac gaggtagaaa aagaaaacgc cgtttttttt ctctttttaa
     1441 aataaaaaga gaaactttcc agactatttt gtttttgttt tttttcatgg cggcaataat
     1501 cacggttttt tctttcttag aaatcggacc aataccgact aaaattagtt taacattaca
     1561 gaattatttc ggatttttag ctgtttttat tccattttta tttctactta ccgcctttct
     1621 ttttgttaaa gttaaatttc ctctgaaaga accaaatatt tttttagggt ttgtaatcat
     1681 gtttattgct ttgatttctc tttttcgagc cggtatcgtt ggtaattatt gccgtgactt
     1741 attaatcaat tcctttggcg aactaattag ttcgatagtt tattttatct gtttaattgc
     1801 cggttttgtt attcttttcg atactaatgt tgcccagctg gtaaaatttt gcgtgaagat
     1861 ttttaaagcc attaaaaaat ataccgttgg tagtggagtt aaatcaacgg taagaaggga
     1921 aaaaatagtg cctcggttgg gttcttcctt accgcctaaa gacgaaacaa aggatgaaaa
     1981 gcaggtaaag atagaagatt atgttattaa tgcccccttt aaatcaccgg tgccggtagg
     2041 tagtaatcaa atttggcaat atccgccgat aactttattt gataacagtt tgggggcgcc
     2101 agccgatcgg ggtaatgttg gcaagaatgc caagactatt gaagagactc tagattcttt
     2161 cggtattacc gctaaagtag ttgaaattaa cgacagcccc tcagttactc agtatgcctt
     2221 agaggtagct ttggggacaa aattggctaa aattgtttcc ttaagtaatg atttggcca
//
LOCUS       DAOOIE010000086         2278 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_37252_length_2278_cov_3.765632, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000086 DAOOIE010000000
VERSION     DAOOIE010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37252_length_2278_cov_3.765632"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..687
                     /locus_tag="PK131_02560"
     CDS             10..687
                     /locus_tag="PK131_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPD45033.1"
                     /translation="MIELKGIKREERDSWPEGITVDNCGLFISSNADYLKKRQVTRFL
                     EDNWGQYGDKIATVIPSYGHNKFFIIRFVSPEPAQEILKDYRGHPDHYQLTDEKELYI
                     LPRYWRKLMKEEKEKGIDFHRLFLNHFEKNFRQDELYNLFGQFGGVLNTFLTHSKGDH
                     QLIGFVLFTTTEAARKALDCLKQEPMGFEAVYARIKNPEKENLIIFKPGEKITSLPRV
                     DLEEFLK"
     gene            713..2047
                     /gene="aspS"
                     /locus_tag="PK131_02565"
     CDS             713..2047
                     /gene="aspS"
                     /locus_tag="PK131_02565"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HPD45034.1"
                     /translation="MKRIWIEDTPAAIGQKVKVAGRIISLRSHGQLVFLDLSDLSGVI
                     QVVGYRDLAKLKMEDMVEIVGLIKSRDKKNFNPKIVTGQIELAAEQFQILSKSADLPF
                     DLHQPQLNVTLPTLLDYRPVSLRHSRIQHIFKVQETIVDTFRSYLKNQNFTEIFVPTI
                     VSAATEGGSELFSLDYFGKKAYLSQSPQFYKQIMVPVFERVFTVAHAYRAEPSVTTRH
                     LTEYVSLDVEMGFIDNWRELIKTADEVIKTLFRTVADKYADVLAEYNVKIPRTIRETP
                     AITLQEAEEIIYKRTKREVRQEPDLDPEGEREIGRYAVEKFNSDLIFITHFPTSKRPM
                     YTFPDPQNPELTLSFDLIGLGTEWITGGQRINDYEQLKRSIQQRGYNPDNFEIPYLQA
                     FKYGMPPEGGFAIGLERITQGLLGLDNIRQASLFPRDMERIDSRLTNRTNEK"
     gene            2037..>2278
                     /locus_tag="PK131_02570"
     CDS             2037..>2278
                     /locus_tag="PK131_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45035.1"
                     /translation="MKNKTVFIGLVIADCFFVFFLAAYLIHYPPIRVKADSKIPIDYQ
                     ETGRPVVINKDHFPILDSQSYILVDAETNTVLAQKNS"
BASE COUNT          697 a          366 c          470 g          745 t
ORIGIN      
        1 taatgtagta tgatagaatt aaaggggata aaaagggagg aaagggattc ttggccggaa
       61 ggaattacag ttgataattg tggtcttttt atatcttcaa atgcggatta tttaaagaag
      121 aggcaggtta ctcggttttt ggaagataat tggggccaat atggggataa aattgcgaca
      181 gttatcccga gttatggaca taataagttt tttataatta gattcgtttc tcccgaacca
      241 gctcaggaaa ttttaaaaga ttatcggggc catcctgatc attatcaatt aactgatgag
      301 aaagaactat atattttacc gcgctattgg cgtaaattaa tgaaagagga aaaagaaaag
      361 gggattgatt ttcatcggtt atttttaaat catttcgaga aaaattttcg ccaagatgaa
      421 ctgtataacc tttttggtca atttggtggg gttcttaaca cctttttaac tcactccaaa
      481 ggtgaccatc agctaattgg ttttgttctt tttactacca ccgaggcagc ccgaaaagcc
      541 cttgattgtc ttaaacagga accaatgggt tttgaggcgg tttatgctcg gattaagaat
      601 cccgagaaag aaaatttgat aatttttaaa cccggagaaa aaataactag tttgcctcgg
      661 gttgatttgg aagagttttt aaaataaata gatagttttg ttatactcat atatgaaaag
      721 aatttggatt gaagatactc ctgccgccat tgggcagaaa gttaaagtag ccggtcgaat
      781 aatttctctt cgctctcatg gtcaattagt ttttcttgat ttgagtgatt taagtggtgt
      841 tatccaggtg gttggctatc gggatttagc caagctgaaa atggaggata tggttgaaat
      901 tgttggctta atcaaatcca gggataaaaa gaatttcaat cccaaaattg ttaccgggca
      961 aattgaatta gcggctgagc aatttcagat tttatcaaaa tcagccgatt taccctttga
     1021 tcttcatcaa ccacaattaa atgttacctt gccgacgctt ttagattatc ggccggtttc
     1081 tctccgtcat tctcggattc agcatatttt taaagttcaa gaaacaattg tcgatacttt
     1141 tcgttcttat ctaaaaaatc agaattttac cgaaattttt gtgccaacaa tcgtttcagc
     1201 tgcgactgag ggtggttctg aattattttc tcttgattat tttggtaaaa aagcttatct
     1261 gtctcagtca ccccaatttt ataagcagat aatggtgccg gttttcgaaa gagtttttac
     1321 cgttgcccat gcttatcgag ctgaaccatc ggtcactact agacatttaa ctgaatatgt
     1381 ttctttagac gtagaaatgg ggtttattga taattggcga gaattaataa agacagctga
     1441 tgaagtaatt aaaacgttgt tccggacggt ggctgataaa tatgccgatg ttttggctga
     1501 atataatgtt aaaattcctc ggaccattag ggaaacgccg gcaattaccc tccaggaagc
     1561 cgaagaaatt atttataaaa gaactaaaag agaagtaaga caggaacctg atcttgaccc
     1621 tgaaggagag agggaaattg gtcgttatgc tgtggaaaaa tttaacagcg atttaatttt
     1681 tattactcat tttcccactt caaaacggcc gatgtatact tttcctgatc ctcaaaatcc
     1741 cgaattaact ttaagctttg atttgattgg tttaggcact gaatggatta ccggcggtca
     1801 gagaattaat gattacgaac aattaaaaag aagtattcaa caacggggtt ataatcctga
     1861 taattttgag ataccctatt tacaggcgtt taaatatggt atgcctcccg agggtggctt
     1921 tgccattggt ctagaaagaa taacccaagg tcttcttggt ttagacaata ttaggcaagc
     1981 tagtttgttt cctcgagata tggaaagaat tgattcccga ttaactaatc gaactaatga
     2041 aaaataaaac ggtttttatc ggcttggtta tagcagattg tttttttgtt tttttcttag
     2101 cggcgtattt gattcattat cctcctatta gagttaaggc ggattctaaa ataccgattg
     2161 attatcagga gactggtcgg ccggtagtga ttaataagga tcattttccc attctcgatt
     2221 ctcagtccta tattttggtc gatgccgaaa ctaatactgt tttagcccaa aagaattc
//
LOCUS       DAOOIE010000087         2252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_37840_length_2252_cov_3.563951, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000087 DAOOIE010000000
VERSION     DAOOIE010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37840_length_2252_cov_3.563951"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="PK131_02575"
     CDS             complement(<1..384)
                     /locus_tag="PK131_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD45036.1"
                     /translation="MVSSKSLRPQTLSEFIGQEPVKHQLHIFLKAAKDRGDSLDHILF
                     YGPPGLGKTTLAMLIAKEMAVNIKTTSGPALTRAGDLASILTALKKGDILFIDEIHRL
                     NKNVEEALYNAMEDYVLDIILGKGPG"
     gene            complement(363..932)
                     /gene="ruvA"
                     /locus_tag="PK131_02580"
     CDS             complement(363..932)
                     /gene="ruvA"
                     /locus_tag="PK131_02580"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPD45037.1"
                     /translation="MIASLQGRVERVNNGQIEIEVNGVGYLITLGERLVKDIIEGTII
                     KLKTYLSISENNLALFGFKTDQEVNLFRMLITVPGVGPKTGSQILGKAEAEVIIRAIS
                     EADVDFFSEIKGIGRKTAQRIIIDLKPKLGSFKELDLIKNGELLEDDLYLALQQLGFE
                     GKEIKKVVSKLPKDILTIEEKLQWCLQNL"
     gene            complement(939..1400)
                     /gene="ruvC"
                     /locus_tag="PK131_02585"
     CDS             complement(939..1400)
                     /gene="ruvC"
                     /locus_tag="PK131_02585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPD45038.1"
                     /translation="MILGIDPGLAGTGWAVLEEEELIDCGWIKTQSNENITVRLGKIC
                     DIIERLIVKYKIENIALENLFFTKNISSALRVSQTIGAIRATGFKRGIKLFEYTPLQI
                     KSTLVGYGRAEKSQVELMVRNILGIKQPIKPSHTADAIAVALTHLYIKHEV"
     gene            1546..>2252
                     /locus_tag="PK131_02590"
     CDS             1546..>2252
                     /locus_tag="PK131_02590"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPD45039.1"
                     /translation="MKFLKNIPLAPYTTVKIGGPAEYFLEVTSYHQFKAVLEGLKKRH
                     IKKITVLGNGSNVLISDQGISGYVIKNSASAIKLLPHHQIWAASGTQLAKLIEFSLKH
                     GLVGLESFAYIPSTLGGAIQGNIHSPECLFSTITKSTEIFDNEYPIILSAVIQLKPGN
                     TAVAYQKVQKIVNQKIKTQPMNSLGCIFKNPSRLPADMPLEPAGSIIDKRLKLKGLCI
                     GDAQISPIHANFITNNN"
BASE COUNT          698 a          494 c          316 g          744 t
ORIGIN      
        1 ccccggacct ttacctaaaa taatatccaa aacatagtct tccatggcat tataaagagc
       61 ttcctccaca tttttattta aacgatggat ttcatctata aataaaatat ctcccttttt
      121 aagagcagtt aaaatagaag ctaaatctcc agcccgagtc aaggccggcc ctgaagtagt
      181 cttaatatta actgccattt ccttagcaat taacatggct aaagttgttt tgcccaatcc
      241 cggtgggccg taaaatagaa tatggtcgag tgagtctccc ctatctttag ccgcttttaa
      301 aaaaatatgg agttgatgtt ttaccggttc ctgaccgata aattccgaca atgtctgtgg
      361 tcttaaagat tttgaagaca ccattgcaat ttctcctcaa ttgttaaaat atccttcggt
      421 aacttactaa ctactttttt aatttccttc ccttcaaaac ctaactgttg caaggctaga
      481 tacagatcat cttccaatag ttcaccattt ttgattaaat ccaactcttt aaaactgcca
      541 agtttgggct ttaaatcaat aataatcctt tgagctgtct tccgaccgat tcccttaatt
      601 tcactaaaaa aatcaacatc ggcttcacta atagccctaa taataacttc tgcttccgct
      661 ttccctaaga tttgagatcc agtttttggt cctacaccag gaacagtaat taacattctg
      721 aataaattaa cctcttggtc cgttttaaag ccaaaaagag ccagattatt ttcagagatg
      781 gataaatagg tctttaactt aattattgtc ccttcaatta tatcttttac tagtctttca
      841 cctaaagtaa taagataacc tactccgttt acttcaatct ctatttgacc attattcacc
      901 cgttcaactc ttccctgaag actagcaatc atactccatt atacctcgtg cttgatatat
      961 aaatgagtca gggcaacggc tatggcatcg gccgtatgag aaggtttaat aggttgctta
     1021 attcccaaga tatttctgac cattagttcc acttgtgatt tttctgccct accataacca
     1081 actaaggttg attttatttg gagtggagta tattcaaaca gcttaatgcc ccttttaaaa
     1141 ccagtagccc taatggctcc aatcgtttgt gaaactctta aagcagaact aatattttta
     1201 gtaaaaaata aattttccaa agctatattt tcaatcttat atttaacaat caaccgttca
     1261 attatatcac atattttacc taatctcacg gtaatatttt cattagactg agttttaatc
     1321 cagccacaat ctattaattc ctcctcttcc aaaactgccc aaccagtacc agctagtcct
     1381 ggatcaattc ctaaaatcat cctaataatt ttaccttatt cgtagttgag ttttttctta
     1441 aacatattat aggctactct atttttaatc acctgtccac atttttccct atactaattt
     1501 attataatat attggcacta gaaaccggcc gtgatatcat tatttatgaa atttctaaaa
     1561 aatattcccc tagcacctta caccactgtc aaaattggag ggccggccga gtatttcctc
     1621 gaggttacct cctatcatca atttaaagcg gttttagaag gtcttaaaaa gcgacacatt
     1681 aaaaagataa cggttttagg taatggctct aacgttctta ttagcgacca aggtatttct
     1741 ggttatgtta ttaaaaattc ggcttcagct attaaattac ttccccacca tcaaatttgg
     1801 gcagcgtcag gaactcaatt agccaaactt attgaatttt cacttaagca cgggttagtt
     1861 ggcttagaaa gttttgccta tatcccctct actcttggcg gcgctatcca aggtaatatc
     1921 catagtcccg aatgtctttt ttcgactata actaaatcga ccgaaatttt tgataatgaa
     1981 tatcctataa tattatcagc tgtcattcaa ctgaaaccag gtaacacagc cgttgcttat
     2041 caaaaagttc aaaaaatcgt taatcaaaaa atcaagacac agccaatgaa ttcccttggc
     2101 tgtatcttta agaacccttc tcgccttccg gcagatatgc cgcttgaacc ggctggttca
     2161 attattgaca aaaggttaaa actcaaagga ttatgtattg gcgacgccca aataagccca
     2221 atccacgcca attttattac caacaataat ca
//
LOCUS       DAOOIE010000088         2250 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_37905_length_2250_cov_3.090661, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000088 DAOOIE010000000
VERSION     DAOOIE010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37905_length_2250_cov_3.090661"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="PK131_02595"
     CDS             <1..457
                     /locus_tag="PK131_02595"
                     /inference="COORDINATES: protein motif:HMM:NF024731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPD45040.1"
                     /translation="KRECRCTLHQIVNYQKKLSGPIMDRIDLHLNVPPVDVNKLMIDQ
                     SQSNAEPSAVIKQRVITARQIQNKRFAKLAHVHTNSDMKNCHLKAFAQLSDKANLLLK
                     QAVNKYALSARTYFRLIKVARTIADLAGSAVISDIHVAESLQYRIRTSE"
     gene            483..746
                     /locus_tag="PK131_02600"
     CDS             483..746
                     /locus_tag="PK131_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45041.1"
                     /translation="MINILIGFSGGLIRGLVGFIKNKTFEKTTQFKIQYFLAAVLISG
                     IVGGAAGLLADGAWQIAFLAGYAGADFLEGLYKIKLGQQLAAK"
     gene            complement(772..1761)
                     /locus_tag="PK131_02605"
     CDS             complement(772..1761)
                     /locus_tag="PK131_02605"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HPD45042.1"
                     /translation="MKSKLKLEEINDLVLLTGTSNQKLAQKIGKILNKARSKPMPVYC
                     PVSHFGDGEIDIFIPENLRRRRVFIIQSTSPPAVNDYIMELAFMADAAKRASAAEVTA
                     VIPYFGYGRQDRKDRARVSICSAVVANILVKAGVDRIVTIDIHANQEQGFVQQPWDNL
                     FASYSLIPRLRQEKIANLVVASPDAGGVKRAKHYEECLKAKGIVIVLKDRDINQHDHS
                     EVLTMVGEVTGKNVLIVDDIISTAGSIAKARDLFRARGAKKIYAAATHGIFSEKALET
                     INGFEKVFVTDTIDHREEVLKHPKIQVVSIAPLLAEAIKRIHTGESISRGLIR"
     gene            complement(1765..1836)
                     /locus_tag="PK131_02610"
     tRNA            complement(1765..1836)
                     /locus_tag="PK131_02610"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1801..1803),aa:Glu,seq:ctc)
     gene            1858..>2250
                     /locus_tag="PK131_02615"
     CDS             1858..>2250
                     /locus_tag="PK131_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45043.1"
                     /translation="MYFIKYKSQILLIFLTLIATLSLWLTFYYQLPQKIGWPAVSLET
                     VFANYDGPNYLAISKCGYDKNCLGPNFSLSQPLEYYPAHFPGYPMLIRFFSNFTTGPK
                     AMLLVTLLGSILLTLTSYALFKSFWLSFF"
BASE COUNT          613 a          474 c          459 g          704 t
ORIGIN      
        1 taaaagagag tgccgttgca ctctgcatca aatcgttaat tatcagaaaa aactttctgg
       61 tccgattatg gatcgaattg acctgcattt aaatgtgccg ccggtggatg ttaataaact
      121 aatgattgat cagtcacaat caaatgccga accatcggct gttattaaac agcgagttat
      181 aactgcccgc cagattcaaa ataaacgttt tgctaaatta gcccatgttc ataccaattc
      241 cgatatgaaa aattgccact taaaagcatt cgcccaatta tcggacaagg ccaatttgct
      301 tttaaagcaa gccgttaata aatacgctct ttctgcccgg acttattttc gcctgattaa
      361 ggttgctcga acgattgccg atctagctgg ttcagccgtt attagtgata tccatgttgc
      421 cgaatcactt caatatcgga tcagaacgag tgaatgaaat aaaattcagt tataattcaa
      481 ttatgattaa tattttaatc ggatttagtg gaggccttat caggggtttg gttggtttta
      541 taaaaaacaa aacttttgaa aaaaccactc aatttaaaat ccaatatttc ctggcggccg
      601 ttttgatctc gggtattgtc ggcggcgctg ccggtctgct ggccgatggt gcttggcaaa
      661 ttgcttttct ggcaggttat gccggagctg attttctcga aggtctctat aaaatcaaat
      721 taggtcagca attagcggcc aaataatctt cttttatgtt aaagaggggg tttatctgat
      781 taaaccccga ctgatagatt cgccggtatg aattctttta atcgcttcag ctaaaagcgg
      841 ggcaatggaa actacttgga ttttaggatg tttaagcact tcttcccgat gatcaatagt
      901 atcggtgaca aacacttttt caaaaccatt aatggtttcc aaggcttttt ccgaaaaaat
      961 gccgtgggtt gccgctgcgt agattttttt ggcgccgcgg gcccggaaga gatctcttgc
     1021 tttggcaatc gaaccggcag tgctgataat atcgtcgact atcagaacat tttttccggt
     1081 gacctcgcca accatggtta aaacttcgga atgatcatgt tgattgatat ccctatcttt
     1141 caaaacgata acaatgcctt tggctttcaa acattcctcg taatgcttgg ctcttttgac
     1201 tccgccggca tcgggggaag cgacaactaa attggcaatt ttttcctgcc gcaatcgggg
     1261 aattaaagaa tagctggcaa acaaattatc ccaaggctgt tgaacaaatc cctgctcctg
     1321 attggcatga atgtcaatgg tgacaattcg atcaacgccg gctttgacca aaatattagc
     1381 aacgacggct gagcaaatag agaccctagc cctgtctttt ctatcctgcc ggccgtagcc
     1441 aaaataggga atgacggcgg taacttcggc cgccgaggct cttttagcgg catcagccat
     1501 gaaagccaat tccataatgt aatcattgac cgccggtggt gaggtcgatt ggatgataaa
     1561 cacccgacgg cggcgaagat tttcaggaat aaaaatgtcg atttcaccat cgccgaaatg
     1621 ggaaacagga caataaacgg gcattggttt agaccgggct ttatttaaaa ttttacctat
     1681 tttttgggct aatttttggt tagatgttcc tgttaataaa acaaggtcgt tgatttcttc
     1741 caactttagt tttgatttca tcagtgactc cacggggaat tgaaccccgg ttgccaggat
     1801 gagaacctgg cgtcctagcc gctagacgat ggagccgatc atgtataatt aactcagatg
     1861 tattttatca aatataaatc tcagattctt cttattttct tgactttaat cgctacttta
     1921 tcactctggc tgacttttta ttatcaactg cctcaaaaaa taggctggcc ggcagttagt
     1981 ctggaaactg tttttgctaa ttacgacggc cctaattatt tggctattag taaatgcggc
     2041 tatgataaaa attgccttgg ccctaatttt tctttatccc aaccgttgga atattatccg
     2101 gctcattttc ccggctaccc aatgttaatc cgatttttca gcaattttac taccggcccg
     2161 aaagcgatgc tgttagttac cctacttggt tcaattttat taactttaac ttcttatgcc
     2221 ctgtttaaat ccttttggct ttcttttttt
//
LOCUS       DAOOIE010000089         2227 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_38473_length_2227_cov_4.006446, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000089 DAOOIE010000000
VERSION     DAOOIE010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38473_length_2227_cov_4.006446"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PK131_02620"
     CDS             complement(<1..459)
                     /locus_tag="PK131_02620"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HPD45044.1"
                     /translation="MANTSIVTNQKKFLFKLSSQEPTPEASWYVINTYSGHEYKVIEA
                     LKTRIKTMALENKVFTAVVPIQSKMIIRRGQKIKSQEKTFPGYVLIQMVLTDDSWVTV
                     RTTQGVTGFVGINNKPSPIAQTEVDQILQTTKEDKPKYQTPFFVGDVVKIT"
     gene            complement(473..721)
                     /gene="secE"
                     /locus_tag="PK131_02625"
     CDS             complement(473..721)
                     /gene="secE"
                     /locus_tag="PK131_02625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPD45045.1"
                     /translation="MQRIIQFIKEVIAEFRHISWPSKEALIQLTSVVIFISIVISLIL
                     GGFDYLFTRSFASLGKYKKQPVQELIQPTPVPTSVMMN"
     gene            768..1310
                     /locus_tag="PK131_02630"
     CDS             768..1310
                     /locus_tag="PK131_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HPD45046.1"
                     /translation="MNQKIDKDEYYLKIAEAIALKSNCLSAEYGALIVKDDAMISAGY
                     VGAPRKTMDCIDLGYCIRRKNRVPSGTGYEMCRSVHAEMNAIINAARAGVSVLGGDMY
                     IHAKTKMGVKEPTLINAYPCLLCKKMIINAGLKRVICNNQDGSHSVYLVEDWIKDWQE
                     IEDIRQDKVKYGVDYYNQKN"
     gene            1381..1848
                     /gene="nrdR"
                     /locus_tag="PK131_02635"
     CDS             1381..1848
                     /gene="nrdR"
                     /locus_tag="PK131_02635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HPD45047.1"
                     /translation="MLCPYCAYDESNVIESRDALDGRATRRRRECLRCGKRFTTYEKV
                     GDIELKVIKRDGRIEPYRREKLERCFEKACWKLNAEERSKLVDQIEMKLLNWPSVEIP
                     SQEIGKMILELLKKIDAVAYIRFATIFLKIQSVDEFEKLIKSYKSKNVSSKII"
     gene            1826..>2227
                     /locus_tag="PK131_02640"
     CDS             1826..>2227
                     /locus_tag="PK131_02640"
                     /inference="COORDINATES: protein motif:HMM:NF015442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HPD45048.1"
                     /translation="MSLQKSFKIETTVKRIKKRNGKIVKFVPSKIIRAIQGAFKSAGE
                     EGDHQAEKLAREVISEVEKRFNQKVIPEVEQVQDLIESVLKKNDYDEVYNAFRLYREL
                     HRKLRMISSLVDSDELIDKYLSRADWRVKENA"
BASE COUNT          713 a          311 c          520 g          683 t
ORIGIN      
        1 agtaattttg actacatcac ccacaaaaaa gggggtttga tatttgggtt tatcctcttt
       61 agtagtttgt aagatttgat caacttcggt ctgagcgata ggcgatggtt tattattaat
      121 gccgacaaag ccagtaactc cctgggtagt tctaaccgtg acccatgagt catcagtaag
      181 aaccatttga ataagaacat aacccggaaa agttttttct tgggacttaa ttttttgccc
      241 ccgccgaatg atcatttttg actgaattgg aacaacggcc gtaaaaactt tattctcaag
      301 ggccatagtc ttgattctag tttttagagc ttcaatgacc ttatactcgt ggccggaata
      361 ggtattgata acgtaccacg atgcttcagg tgttggctct tgagaagaga gtttaaagag
      421 aaattttttt tgattagtaa ctattgaagt attagccatt agtttgttgt ttttaattca
      481 tcataactga agtgggaacc ggtgttggtt gaatcaattc ttgaaccggt tgttttttgt
      541 atttccccag tgacgcaaaa gaacgagtaa ataaataatc gaacccccca agaatgaggg
      601 agataactat cgaaatgaat attaccacag aagttaactg aattaaagct tcttttgagg
      661 gccagctgat atgtctaaat tcagcgatga cttccttaat aaattggata attcgttgca
      721 tgcgtttaat tataccgtag accttagtaa catgctagaa tgggttcatg aatcaaaaaa
      781 ttgacaaaga tgaatattat ttaaaaattg ccgaagcgat tgctttgaag tccaattgct
      841 taagtgcaga atacggagct ttaattgtta aagatgatgc catgatttca gctggttacg
      901 tgggggcacc aagaaagacc atggattgta ttgatttggg ttattgtatc agacgaaaaa
      961 atagagttcc ttcaggtacg ggctatgaaa tgtgtcgttc ggttcatgct gaaatgaatg
     1021 ctattattaa tgctgctcgg gctggtgttt cggttttggg gggagatatg tatattcatg
     1081 ccaagactaa aatgggggtg aaagagccaa ccttgataaa cgcttaccct tgtttgttat
     1141 gtaaaaaaat gattattaat gctggtttaa aacgggtaat ctgtaataat caagacggtt
     1201 cacattctgt ttatttagta gaagattgga ttaaggactg gcaggagatt gaggatatta
     1261 ggcaagataa ggttaagtat ggagttgatt attacaatca gaaaaattga cttagtttga
     1321 agtaaaaggt aacagttgac agggctatcg gtagtgtcta gtattatacc cagacgctat
     1381 atgttgtgtc catattgtgc ttacgatgaa tcaaatgtga ttgagagccg agatgctctt
     1441 gacggtagag ctacccgccg acggcgggaa tgtttgcgat gcggtaaaag atttactact
     1501 tatgagaagg tgggtgatat cgagttgaag gtaattaaaa gagatggtag gatagagcct
     1561 tatcggcgtg agaagttgga aaggtgtttt gaaaaagcct gttggaaatt aaatgctgag
     1621 gagcgaagta aattggttga tcagatagag atgaagctgt tgaactggcc cagtgtggag
     1681 ataccaagcc aggagatagg taaaatgatt ttagaacttc ttaaaaaaat agacgcggtt
     1741 gcctatattc gttttgctac tatttttcta aaaattcaat cagttgatga atttgaaaaa
     1801 ttaattaaaa gttataaatc taaaaatgtc tcttcaaaaa tcatttaaaa tagaaacaac
     1861 tgtcaaaagg attaagaaac gaaatggcaa gatagttaag tttgttccat ccaagattat
     1921 tcgggccatt caaggagctt ttaaatcggc tggtgaagaa ggggatcatc aggcagagaa
     1981 attagccagg gaagtaatca gtgaggttga gaagagattt aatcaaaaag taattcccga
     2041 ggtggagcag gttcaagatt taattgaatc tgttcttaaa aaaaatgatt atgatgaagt
     2101 ttataatgcc tttaggcttt atcgggaact gcatcggaaa ttgagaatga tttccagtct
     2161 ggttgattcc gatgagttga ttgataaata tttgagcaga gctgattggc gggtcaaaga
     2221 gaatgct
//
LOCUS       DAOOIE010000090         2190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_39453_length_2190_cov_3.087119, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000090 DAOOIE010000000
VERSION     DAOOIE010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39453_length_2190_cov_3.087119"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PK131_02645"
     CDS             <1..507
                     /locus_tag="PK131_02645"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPD45049.1"
                     /translation="CWARIYWSNGYLQHEPGKNTGKTHISAWVSGGSAIIRKEYFEKL
                     GGFDPVYSPFYSEDLDLGLRAWKSGYQLLWEPKAIVEHKHESTMSKFPKRFLNYVQER
                     NRLLSTIRNFTEPKFVYLNRLGQLGRILSGPNYIKIIRAAKKQIATNPPPIVFPIISS
                     QQLLDLFK"
     gene            complement(500..1468)
                     /locus_tag="PK131_02650"
     CDS             complement(500..1468)
                     /locus_tag="PK131_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HPD45050.1"
                     /translation="MKKFDIITFGSGTKDFYFIFDHLSSETIADGIKLSLGAKIEVDR
                     VLALTGGGGTNVAVGLRKLGFTTAWCGMVGNDLSGQEVIHDLENFKVDTSLVLKNNQK
                     PTNQSVILMAPGTERTILVYRGASNELDQNSLPWSKFQTDWFYLAPLGGKNLEFFNPL
                     LDFSCEQKIRVAINPGTRQITFMKTKLREILGKIDILFVNEEEASLITGISSQNLVEI
                     AHELDNLTKGIWVITRGSKGAIASDKKFYYRSGILPTEVIDKTGAGDSFNSGFLGSYL
                     RSGDVVLALQLGTANAAKNLEQWGAKMDLLSLGDEWNQVEVERRPI"
     gene            1505..>2190
                     /locus_tag="PK131_02655"
     CDS             1505..>2190
                     /locus_tag="PK131_02655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPD45051.1"
                     /translation="MSQIELSIIIVTWNTAPITLECLKTIHQYLSDKLSFEVIVVDNA
                     STDNTILEIKNWSASRRIKIINNPQNYGFARACNIGARKAKGIYLLFLNSDMKLIDSS
                     LVKMVEYIKNHSRIGIIGPKFLNPDLTPQGSVFPPQTAINAFKEYILGIKNAYSKYVP
                     TTNQPIPVWSISGGALIIKKELFFQAKGWNEKYFMYFEDLDLCQTIRQLGYLVIFYPR
                     CSLIHAHGQSG"
BASE COUNT          689 a          472 c          349 g          680 t
ORIGIN      
        1 tgctgggcta gaatttattg gagtaatggc tatcttcaac atgaacccgg taaaaatact
       61 ggcaaaactc atatctctgc ttgggtttcc ggcggcagtg cgatcattag aaaagaatac
      121 tttgaaaaat taggtggttt cgatcctgtt tatagccctt tttattcgga agatttggat
      181 ttaggccttc gggcttggaa atcaggctac cagcttcttt gggaacctaa ggccattgtt
      241 gaacataaac atgaatcaac tatgtctaaa tttcccaaaa ggtttcttaa ttacgtccaa
      301 gagagaaatc ggcttttaag tactatcaga aactttactg aacctaaatt tgtttattta
      361 aatcgattgg gacaattagg ccgaattctg tctggtccta attatattaa aattatccga
      421 gcagccaaaa aacaaatcgc cacaaatccg cccccaattg ttttcccgat aatttccagt
      481 caacaattac ttgacctttt taaataggtc gccgttccac ttccacctga ttccattcat
      541 ctcccaaact caaaaggtcc attttagctc cccattgctc caaattttta gccgcattag
      601 ctgtccccaa ttgaagagct aaaaccacat cgccactcct caaatagctg cccaaaaaac
      661 cgctgttaaa agaatcaccg gcaccggttt tatcaatcac ttctgttggt aaaattccac
      721 tccgataata aaatttttta tcggaggcaa tcgctccttt agatcctcgg gtaattaccc
      781 aaattccttt tgttaaatta tccaactcat gagctatttc aactaagttc tgagaagaaa
      841 tcccagttat aagtgaagct tcttcctcat taacgaataa aatatcaatt ttaccaagaa
      901 tttcccttaa ttttgtcttc ataaaagtta tctgccttgt tccgggatta atagcaactc
      961 gaattttttg ctcgcaggaa aaatctaaaa ggggattgaa aaattcaagg tttttgcccc
     1021 ctagaggagc aagataaaac caatcagtct gaaatttaga ccaaggaaga ctattttgat
     1081 ccaattcatt tgaggctcct cgataaacta aaattgttct ttctgttccc ggagccatta
     1141 aaataactga ttgattagtc ggtttttgat tattctttaa aacaagagaa gtatcaacct
     1201 taaaattctc taaatcgtga ataacctcct ggccgcttaa atcattacca accataccac
     1261 accatgctgt ggtgaaaccc agcttcctta agccaacggc tacattagtg ccgccgccgc
     1321 cagttaaagc caaaactcga tctacttcaa tttttgctcc caaacttagt ttaataccat
     1381 cagcaattgt ctctgaagat aaatggtcaa agataaaata aaagtctttg gtgcctgatc
     1441 cgaaagtaat aatgtcgaat ttcttcattt aagcaatgat accacaaggg ctataataga
     1501 gttagtgagc caaatcgagc tttctatcat cattgtcact tggaacactg ctccgattac
     1561 cctcgaatgt ttaaaaacaa tccatcaata tctctccgat aagttatctt tcgaagttat
     1621 cgtcgtcgac aacgcctcca ccgacaatac cattttagaa attaaaaatt ggtctgctag
     1681 ccggcggata aaaattatta ataatcctca aaattatggt tttgcccgag cttgcaacat
     1741 cggcgcccga aaagccaaag gtatttatct tctcttctta aactctgata tgaaattaat
     1801 cgatagcagt ctggttaaaa tggttgaata tatcaagaac cattcccgaa ttggtattat
     1861 cgggccaaaa ttcctcaatc ccgatttaac ccctcaaggt tctgtttttc ctccccaaac
     1921 cgccataaac gcctttaagg aatatatttt aggtattaaa aatgcttatt caaaatatgt
     1981 tccgactaca aaccaaccaa ttccagtatg gtcaatttct ggtggagcgt taattataaa
     2041 aaaagaatta ttttttcaag ccaaaggctg gaatgaaaaa tattttatgt attttgaaga
     2101 tttggatctt tgccaaacta ttagacaatt aggctatctg gtcatttttt acccccgatg
     2161 ttctttaatt catgctcatg gtcaaagcgg
//
LOCUS       DAOOIE010000091         2176 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_39822_length_2176_cov_2.780764, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000091 DAOOIE010000000
VERSION     DAOOIE010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39822_length_2176_cov_2.780764"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..905
                     /gene="dnaN"
                     /locus_tag="PK131_02660"
     CDS             <1..905
                     /gene="dnaN"
                     /locus_tag="PK131_02660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPD45052.1"
                     /translation="ITEFVSYLDPGKIDLTLNSNLLNLKSNKAESDFTTFSPIDFPVL
                     SSANSKDGFEIDFSDLADPINQITFSAATDDTRPVLTGILVCFTKDNLILVATDGFRL
                     SLKEIKLKSPIKMPSGKDRLTLLVPAKALMEINKLVSPNSLIKISIAKDERQIFFIMD
                     NLELVSSLLEGQYPEYQKIIPESFSTTIEIDKDELTQAIKLASVFAAQSANVVKMNIK
                     HNVVEISANAPQLGKNKITVATKINGDPIEIAFNYKFILDFIGAVKGKELVIKLNESL
                     TPGLFADVADPTYTHIIMPVRIQG"
     gene            complement(909..>2176)
                     /locus_tag="PK131_02665"
     CDS             complement(909..>2176)
                     /locus_tag="PK131_02665"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPD45053.1"
                     /translation="YDLIKGRMLDLKTALPYSLSLSQQIDQWVTYLKNQPKLQERLKQ
                     VQNWNHFWRKLGLPIVNQDVGWILQNRVATHFQRDLTMGINTGIKGVLQATSGVAAPF
                     IVIGNIIANGFKKILNNLGINQGEIKRWLGHNLNHLGHNFLNGALKFTEGLATMPGLA
                     LAAVTPKIITVIVVAVVVGLFVYQIYTGSLISGLVPPTAYGGQEQPDVTITPTLTPGI
                     SPLPSSKAICYIERILGGKKTLMPVFDKNGGRGLNFIIYDIGYYDTYPGIFNVQKSLN
                     TKKLAKTKYGGGNNYSDYLEPNVAQILDVMLDDLLKEKGTDYKDLILLDAAYRDYDQQ
                     ALLRANSNWAEAPGQSQHHTGRALDFHFGGYDSSGNPRPLTAHEQVVYNWLVKNAEKY
                     GFYQTYDSEKWHWFYNPKVNSGENCKKLD"
BASE COUNT          618 a          416 c          428 g          714 t
ORIGIN      
        1 aaattacaga atttgtttct tatcttgatc cgggcaaaat tgatttaact cttaatagta
       61 atcttttaaa tctaaaatca aataaggccg aatctgactt tactaccttt tccccaattg
      121 attttccagt tctttcatca gctaattcta aggatggttt tgagattgat ttttctgact
      181 tagcagaccc cattaaccaa attacctttt cagcagcaac tgacgatact cgtcctgttc
      241 taacaggtat tttagtttgt tttactaaag ataatttaat tttagtggcc actgatggtt
      301 tccgtttgtc attaaaggaa attaagctta aaagtccgat taaaatgcct tcaggtaaag
      361 atagattaac tcttttagtt cctgccaaag ccttgatgga aataaataaa ttagtttcgc
      421 ctaattcatt gataaaaatt agtattgcta aagatgaacg acaaatattt tttatcatgg
      481 ataatttaga attagttagt agtcttttgg aaggtcaata ccctgaatat cagaaaatta
      541 ttcctgagtc attttcgacc actattgaga tagataaaga tgaattaacc caagctatta
      601 aactcgcctc tgtttttgca gctcagagtg ctaatgtggt gaagatgaat attaagcata
      661 atgtggtgga aatttcagct aatgctcctc agttgggcaa aaataagatt actgtcgcca
      721 ctaagattaa cggtgatcct atagagattg cttttaacta taaatttatt ctggatttta
      781 taggagcggt caaaggtaag gagttggtta ttaaattaaa cgaatctctt accccaggtt
      841 tatttgccga cgtagctgat ccaacctaca cccatatcat tatgccggtc agaattcagg
      901 gctgagagtt aatctaattt tttgcaattt tcacccgaat tgacctttgg attataaaac
      961 cagtgccatt tttcgctgtc gtatgtttgg tagaaaccat acttctcggc attttttacc
     1021 agccagttgt aaactacctg ctcgtgggct gtcagtggtc tagggttgcc actagagtca
     1081 tatccgccga aatgaaagtc tagggccctg ccggtatggt gttgggattg gcccggagct
     1141 tcagcccaat tagaattagc cctcaaaaga gcctgctggt catagtctct ataggccgcg
     1201 tccagtaaaa ttagatcttt ataatcggta cccttttctt ttagtaaatc atccagcatt
     1261 acgtctagaa tttgggctac gttcggttct aaataatcgc tatagttgtt accgccgcca
     1321 tattttgttt tagctaactt tttggtgttg agtgatttct ggacgttgaa gataccggga
     1381 taagtgtcat aataaccgat gtcataaatg ataaaattta atccccggcc gccgtttttg
     1441 tcaaaaaccg gcattaaagt ctttttacct cccaaaattc tttctatata gcagattgct
     1501 tttgagctgg gaagaggact aatgcctggt gttagggttg gggtgatggt tacgtccggt
     1561 tgctcttggc cgccgtaggc ggttggcggc accaaaccag agattaaact gccggtgtaa
     1621 atttgataaa caaataaacc aacaaccaca gccacgacaa taacagtaat tattttagga
     1681 gtgacggcgg ctaaagccag ccctggcata gtggctaaac cttcagtgaa tttaagagct
     1741 ccatttaaaa aattatggcc caaatggtta agattatgcc ctaaccacct tttaatttct
     1801 ccttggttta tgcctaaatt attaagaatt tttttaaatc cattggcgat tatattgccg
     1861 atgactatga aaggggcagc taccccactg gttgcctgca ataccccctt aatgccggta
     1921 ttgataccca tggttagatc cctttgaaag tgggttgcaa cccggttttg aagtatccat
     1981 ccgacgtctt gattaactat ggggagtcca agttttcgcc agaaatgatt ccaattttgg
     2041 acttgtttta gcctctcttg aagcttgggt tgatttttaa gataggtaac ccactgatct
     2101 atttgttgac ttaaggagag agaatagggt aacgctgtct ttagatccaa catccgccct
     2161 ttgatcaagt cgtatt
//
LOCUS       DAOOIE010000092         2116 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_41396_length_2116_cov_3.305192, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000092 DAOOIE010000000
VERSION     DAOOIE010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41396_length_2116_cov_3.305192"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1521
                     /locus_tag="PK131_02670"
     CDS             <1..1521
                     /locus_tag="PK131_02670"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPD45054.1"
                     /translation="LADDCSTDQSVRETILAYAQHDPRIKYVFRETNGHISLCSNSAL
                     AISKGEFICLLDHDDELFPNALFEIVKLLNKDRHLDLIYTDEDKIEENGRHVDPFFKP
                     DWSPDMFLSVNYLCHLTAIRRSLVKQIGGFRPGFEGSQDYDLFLRVTELTENIAHLPK
                     ILYSWRKTPGSTAAVYEAKSYADQASIRALTEALKRRKIKGQVTDGLVSGTFRVRYDL
                     HRQPLVSIILPTKNQYKYVKKCLKSVLEKTKYPNYELVIIDTGSNDKKVWRFYETIKR
                     EHEETQILTWNKPFNFSAVCNWGAAKSKGQYYLFLNNDTEVVTPDWIENMLEHAQRKE
                     VGAVGVKLLYPNKTIQHAGVVLGIIGEHNRRKKGVAGHVLKYFPDVPLRNSLLNSKDT
                     IRNYSAVTGACLMVKKEKFFEVGGLDEKFRIAFNDVDFCLKLGAKGYLNVYTPYSRLY
                     HYESISVGKPEKLTRNIKEFKKEQRLMVKKYGTILINDPFYNQNLTLFDEDLSIKI"
     gene            1518..>2116
                     /locus_tag="PK131_02675"
     CDS             1518..>2116
                     /locus_tag="PK131_02675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPD45055.1"
                     /translation="MNQPLVSVIIYAYNPKEKLLDRCLKSILEQTYRRIEIILVNDGS
                     KPKFQSIMKRYQSQDNRIIYVEHEKNLGLFQARITGYKKSRGELITFVDSNDYFYSID
                     YIRRLVDNAIKTNADITFGNFFLEENKRKVIFNISDLPADQVISNQNRALLSLYFAQR
                     GLNFAWSLIISKLYTRRLWTRALPYLEKQKEHLVMLEDIA"
BASE COUNT          714 a          354 c          407 g          641 t
ORIGIN      
        1 ttggctgatg attgttcaac cgatcaatcg gtcagagaaa ctattctggc ctatgctcaa
       61 cacgatccaa gaataaagta tgtttttagg gaaacaaacg gccatatttc attatgctct
      121 aattcagctt tagccatcag taagggagaa tttatatgtt tattagatca tgacgatgaa
      181 ttgtttccta atgctctttt tgagatagtt aaattgctaa acaaagatcg tcatctcgat
      241 ttaatttata ccgacgaaga caaaattgag gaaaacggcc gacatgttga tccttttttt
      301 aaacccgatt ggtctccaga tatgtttttg tcggttaatt atttatgcca tctaactgct
      361 atcagacgta gtttagtgaa gcagattggc ggctttcgtc ccggctttga gggttcacag
      421 gattacgatc tttttttgag agtgacggaa ttaactgaaa atattgccca tttacctaag
      481 atattatatt catggcggaa aaccccaggt tcaacagccg cggtttatga agctaaaagt
      541 tacgctgatc aagcctcgat tcgagcctta acggaagcat taaaaagaag aaaaataaaa
      601 ggtcaagtga ccgatggcct tgtttcgggg acttttcgag ttcgatatga tcttcatcgt
      661 caaccattgg taagcattat tcttcccact aaaaatcagt ataaatatgt taaaaaatgt
      721 cttaaatcgg ttttagagaa aaccaaatac cctaattacg agctggtaat tattgatacc
      781 ggatcaaatg ataaaaaagt ctggcgtttt tacgaaacaa taaaaaggga acatgaagag
      841 actcaaatat taacatggaa taaacctttt aatttttcgg cggtttgcaa ttggggcgcc
      901 gccaaaagta aaggccagta ttatctattt ttgaataatg atactgaagt ggtgactcct
      961 gactggattg aaaatatgct ggaacacgcc caaagaaaag aagttggggc ggttggagtg
     1021 aaattattat atccaaataa aacaattcag cacgccggag ttgttcttgg cattattggc
     1081 gaacacaata gaaggaaaaa gggagttgcc ggtcatgttc taaagtattt tcccgatgtg
     1141 cctctaagaa attcattatt aaactctaaa gataccatta ggaactactc agctgtaacc
     1201 ggtgcttgct taatggttaa aaaggaaaaa ttctttgagg ttgggggact agacgaaaaa
     1261 tttcgaatag cctttaatga cgttgatttc tgtctaaaat tgggggctaa aggttattta
     1321 aatgtttata ccccatattc tcgactatat cattatgagt caatttcggt aggaaaacca
     1381 gaaaaattaa ccaggaatat aaaggaattt aaaaaagaac aacggctgat ggttaaaaaa
     1441 tatggtacaa tactaataaa tgacccattt tacaaccaaa accttacctt atttgatgaa
     1501 gatttgtcga taaaaatatg aaccaaccct tagtttcggt catcatctat gcttataatc
     1561 ccaaagaaaa attacttgac cgctgtctta aaagtatttt agagcagaca tatcgccgga
     1621 tagaaattat tttagtgaac gatggctcca aaccaaaatt tcaatcgata atgaaacgtt
     1681 atcaaagtca agataacaga attatttacg tcgaacatga aaaaaattta ggcttgtttc
     1741 aagccagaat taccggttat aaaaaatcta gaggagagtt aattactttt gttgacagca
     1801 atgattattt ttattcaatc gattatatta gacggttagt cgacaatgcg attaaaacca
     1861 atgccgatat tactttcggc aatttcttct tagaagagaa caaaagaaaa gtaattttta
     1921 atatcagcga tttgccggcc gatcaagtta tttctaacca gaatcgggcc ttattgtctc
     1981 tatattttgc ccaaaggggt ttaaattttg cctggtcgct gataattagc aaactttata
     2041 ccaggcgttt gtggactcgg gctctaccct atttagagaa gcaaaaagaa catttggtca
     2101 tgctcgagga tattgc
//
LOCUS       DAOOIE010000093         2112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_41512_length_2112_cov_2.914925, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000093 DAOOIE010000000
VERSION     DAOOIE010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41512_length_2112_cov_2.914925"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PK131_02680"
     CDS             <1..305
                     /locus_tag="PK131_02680"
                     /inference="COORDINATES: protein motif:HMM:NF017317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Holliday junction DNA helicase RuvB C-terminal
                     domain-containing protein"
                     /protein_id="HPD45056.1"
                     /translation="DYAQIKNKNLITLKETMEALVMLGVDELGLNETDRKYLDVVKKQ
                     YGGGPVGVENIAAALTEDIGTITEVYEPYLMKKGLIKRTPRGRVLVEKGLFSLNSQ"
     gene            complement(282..527)
                     /locus_tag="PK131_02685"
     CDS             complement(282..527)
                     /locus_tag="PK131_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPD45057.1"
                     /translation="MTDQTLSQDNSVKPKKVNGVSIDNLPDLMTVGEVADLLRVSKLT
                     IKRWGKRGKLPAIRINARGDRRYRKEAVLWLLGIQRE"
     gene            complement(605..1081)
                     /locus_tag="PK131_02690"
     CDS             complement(605..1081)
                     /locus_tag="PK131_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HPD45058.1"
                     /translation="MTKNELVNQTEVWLVNQKLKYNNKDLNRPWGAFWNIAPDSTRRF
                     LQLFFPDFNSKDRSLSPKILLIEPQKQLSLQLHFHRSEKWFVINGPVKVTQGNKQFIL
                     NRHQTVILEPQIPHRLSGLKQPGLVAEIWIHTDPHHPSTENDELRLEDDFHRSNPS"
     gene            1107..>2112
                     /locus_tag="PK131_02695"
     CDS             1107..>2112
                     /locus_tag="PK131_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPD45059.1"
                     /translation="MKPVIICGGIGTKMWPLSCWKMPKQFLPLINGKSLFQLNWEALR
                     LKFKPNEIYLQTNKEQAVIANNQISEIIDENIFIEPEMKNQGPATGFAAAMLYRQFPD
                     EPFMLVQADVLRQPTEKFLEMIDQFDNLIRQKGKIVTGGMKPKEAVMGVDYLKINKTT
                     RQMEEWLGRGEKEQIESYLTTGDVLIHTNHYAWTPRLMLDLFKRRKPAWYQPLVNIIN
                     GGNIAVEYAKMPSGPIEEITKEELLNGYIIELPFDWVDFGTWESVADYLVRNGLWQKD
                     SNILEIEGQNNFIKTPSGKKVAIIGLNDLVVIDNGEALLITTKNNSGKVSEAAAYFNS
                     T"
BASE COUNT          707 a          304 c          472 g          629 t
ORIGIN      
        1 gggattatgc tcaaataaaa aataaaaatt taattactct taaggaaacg atggaagccc
       61 tagttatgtt aggagtagat gaattaggtt taaatgagac agatagaaag tatttagatg
      121 tggttaaaaa acagtatggc ggcgggccag taggagtgga aaatattgcg gcagctttaa
      181 ccgaagatat cggaacaatt actgaggttt atgaacctta tttaatgaaa aaaggtttga
      241 ttaaacgaac tccgaggggc cgggtattag ttgaaaaagg tttattctct ttgaattccc
      301 agtaaccaaa gcaccgcttc cttgcgatag cgtcgatcac ctcgagcgtt gattctaatg
      361 gctggcaatt tacctcgttt accccaacgt ttgatagtca gtttagaaac ccgaagaagg
      421 tcggcaacct ctcctactgt cataagatca ggtagattat cgatagagac cccattaacc
      481 tttttaggtt tcacagagtt atcttgtgat agagtttgat cggtcattat agtggctcct
      541 cttatatcaa agtaatgttt aactgtaaag agtaaaacac atatgatact agtatgtcaa
      601 gatatcaaga tggattggag cgatgaaaat cgtcttcaag acgaagttca tcattttcag
      661 tggagggatg atgagggtca gtatggatcc agatttcggc aactaaacct ggttgtttga
      721 ggccactgag acgatgggga atttgaggct ccaatataac agtttgatgg cgattgagga
      781 taaattgttt attaccctgg gtaactttaa ccgggccatt aatgacaaac catttttcag
      841 agcgatgaaa atgtagttga agagagagct gtttttgagg ttcaataagg agaattttag
      901 gactaagtga tcgatcttta gagttgaagt caggaaagaa taattggaga aaacgacggg
      961 tggaatcagg ggcaatattc caaaaagctc cccatggtcg gtttaaatct ttattattat
     1021 attttaactt ttgattaact aaccagactt cagtttgatt gacaagttca tttttagtca
     1081 tttttatatt atgataaata tagcatatga aaccggtgat aatctgtggg ggcattggaa
     1141 caaaaatgtg gcctctgtcc tgttggaaaa tgcctaaaca atttttaccg ctaattaatg
     1201 gaaaatcatt atttcagttg aattgggaag ccttaagatt gaaatttaaa ccgaatgaaa
     1261 tttatcttca aacaaataaa gaacaggcag ttatagctaa taaccaaatt tcggagatca
     1321 ttgacgaaaa tatttttatt gagccggaga tgaaaaacca aggtccggca acaggttttg
     1381 ccgcagctat gctttatcgg caatttcctg atgaaccatt tatgttagtt caagccgatg
     1441 ttttaagaca accaacggaa aaatttttgg aaatgatcga tcaatttgat aatttaatca
     1501 gacaaaaagg taaaatagta actgggggaa tgaaacctaa agaggctgtt atgggagtgg
     1561 attatttgaa gataaataaa acaacgcggc aaatggaaga gtggttagga cggggagaaa
     1621 aagaacagat agagtcatat ttaactacag gtgatgtttt aatccatact aatcattatg
     1681 cttggacacc aagattaatg ttggatcttt ttaaacgacg aaagccggca tggtatcagc
     1741 cgttggttaa tattattaac ggaggaaata tagcggttga atatgctaaa atgcccagtg
     1801 gtccgattga agagattacc aaagaagagt tactaaatgg ttacataatt gaattgcctt
     1861 ttgattgggt tgattttggt acctgggaat cagtagctga ttatttggtt cgaaatggtt
     1921 tgtggcaaaa agatagtaac attttggaaa ttgaaggtca aaataatttt attaaaacac
     1981 cctcagggaa gaaagtagcc atcattggtt taaatgattt ggtggtgatt gataatggcg
     2041 aggccttgct tatcaccacc aaaaataatt caggtaaagt tagtgaagcg gcagcttatt
     2101 ttaattcgac tt
//
LOCUS       DAOOIE010000094         2101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_41840_length_2101_cov_2.518084, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000094 DAOOIE010000000
VERSION     DAOOIE010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41840_length_2101_cov_2.518084"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="PK131_02700"
     CDS             <1..756
                     /locus_tag="PK131_02700"
                     /inference="COORDINATES: protein motif:HMM:NF017847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HPD45060.1"
                     /translation="VVRDFHDVNVVKEGSLDPQSDLEVVWAELIIKDLETIDKYLEQN
                     EKRPENPKKFALAKELKIALEQGKLAVNLDLSEEEKTILKEFFLLTAKPYFIVINVGE
                     ESYKNIENWNLKIGNLNNVVPVCAKLENELAQLSQEDQKTFLASYGLKQSGLERVIPK
                     AYEVLGLQSFYTAGIKEARAWTIKRGATAPQAAGVIHTDFERGFIMAEVTSYDDFVKY
                     CGWDGVKAAGALHHQGKTYIMQPDDVVEFRFNV"
     gene            876..1763
                     /gene="miaA"
                     /locus_tag="PK131_02705"
     CDS             876..1763
                     /gene="miaA"
                     /locus_tag="PK131_02705"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPD45061.1"
                     /translation="MSEPILIISGPTASGKTDLAAKLAKLLDGELVSADSRQIYRGLD
                     IGTGKDRPENVKIHLIDLINPNQSFSVAQYRELALKTIKEIKSRGKLPIVVGGTGQYL
                     EAIINPPKPSFNIKPNKFLRFFLNKFSLKTLQVIYKFLDEESFSKLNYADLHNPHRLI
                     RKIEIKLSHPRKIENCELKVENYYHLSLTAPNRVLYQRVDQRVEARIKNGLEQEIEKL
                     LKKYRWGDPGLNTHAYKELKTGFTEENINRWRFDEHRYVRRQKTWLEKMPNIRYFDIT
                     SPHYPQEVIDYVLKCYNKL"
     gene            1760..>2101
                     /locus_tag="PK131_02710"
     CDS             1760..>2101
                     /locus_tag="PK131_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPD45062.1"
                     /translation="MRPLKFEISLRTIITALLIILGLSLLWRLKTVFLLFFFCLILTE
                     ILNPMVNWIIRFKIPRPAAIIILYLIMTAIISFAVAGIIPIFIEQTSAFLQALPGIFK
                     SLKDIRLFEAIP"
BASE COUNT          682 a          342 c          407 g          670 t
ORIGIN      
        1 gtggttcggg attttcatga tgttaatgta gttaaagaag gaagtcttga ccctcagagt
       61 gatttggagg tagtttgggc cgaactgatt attaaggatt tggaaacaat tgataaatat
      121 ttggaacaga atgaaaaacg gccagaaaat cccaagaaat ttgccctagc caaagagtta
      181 aaaattgcct tagagcaggg taaattagca gttaatcttg atttaagtga ggaagaaaag
      241 actattctta aagaattttt cttgttaact gccaagccct attttattgt gataaatgtt
      301 ggagaggagt cctataagaa tattgaaaat tggaatttaa aaattggaaa tttaaataac
      361 gtagttccgg tttgcgccaa attggaaaat gagttagccc aattaagcca ggaagaccaa
      421 aaaacttttt tagccagtta tggtttgaaa caatctggtt tagaaagagt tatcccaaaa
      481 gcctacgaag tgcttggtct ccaatcattt tatactgctg gaattaaaga agctcgggct
      541 tggactatca aacgtggagc aacggcacct caagccgcag gggttattca cactgatttt
      601 gaaagaggtt ttattatggc tgaagttact tcttacgatg attttgttaa atattgtggt
      661 tgggatgggg taaaagcggc tggagccctt catcatcagg gaaaaactta tataatgcaa
      721 ccggacgatg tggttgagtt tcgatttaat gtttaataaa actaataaaa taattttcaa
      781 aatccaattt ttaaatcctc cctccgccta gacggatgac ttctttgacg aaaggaggtt
      841 taggaggagt ttaattgaaa attcattaaa attttatgag tgaacctatt cttattattt
      901 ctgggccgac ggcttccggc aagaccgatt tagcggctaa attagcaaaa ttattagatg
      961 gtgagttggt gtcagctgat tcccggcaga tctaccgagg actagatatc ggcaccggta
     1021 aagatcggcc tgaaaatgtc aaaattcatt tgattgactt aatcaatccc aatcaatcct
     1081 tttcggtggc tcagtataga gaattagccc ttaaaactat taaggaaata aaatctcggg
     1141 gcaaactgcc aatagtagtc ggcggcaccg gccaatatct agaggcaatc atcaatccgc
     1201 ccaagccgag ttttaacatt aaaccgaata agtttttgag gttttttctc aataaatttt
     1261 ctttaaaaac tttacaggtt atttataaat ttttggatga agagagtttt tcgaaattga
     1321 attacgccga tcttcacaat ccccatcgtc taattcggaa aatagaaatt aaattatctc
     1381 atcctcgaaa aattgaaaat tgtgaattga aagttgaaaa ttattatcat ctttccctaa
     1441 ctgctcccaa tcgagtttta tatcaacggg tggatcaaag ggttgaagca agaattaaaa
     1501 atggtttaga gcaagaaatt gaaaaattac ttaaaaaata tcggtggggt gacccaggtt
     1561 taaatactca tgcttacaag gaattaaaga ccggttttac agaagaaaat attaatcgtt
     1621 ggcggtttga cgaacaccgc tatgtccgcc ggcaaaaaac ctggcttgaa aaaatgccta
     1681 atataagata ttttgatatc acttcacctc attatcccca agaagttatc gattatgttt
     1741 taaaatgtta caataaacta tgaggccgct aaaatttgaa atttctcttc gaacaattat
     1801 tactgccctt ctaattattt taggattaag cttactttgg cggcttaaaa ctgtttttct
     1861 acttttcttc ttttgtttaa ttttaacgga gattttaaat cccatggtta attggattat
     1921 tcggtttaaa attcctcggc ctgccgcgat tatcattctt tatctgataa tgacagcaat
     1981 tattagtttt gccgtggctg gtattattcc tatttttatc gaacaaacta gtgcttttct
     2041 tcaagctttg ccaggaatct ttaaaagttt aaaagacatt agactatttg aggcaatacc
     2101 t
//
LOCUS       DAOOIE010000095         2095 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_42004_length_2095_cov_2.965686, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000095 DAOOIE010000000
VERSION     DAOOIE010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42004_length_2095_cov_2.965686"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PK131_02715"
     CDS             complement(<1..249)
                     /locus_tag="PK131_02715"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPD45063.1"
                     /translation="MIDTHAHLAKILPETRGKVKWIILAAASRKESFRNLELAQKYDF
                     LIPAVGIHPMSPFEKIDDLIKKAKAIGECGLDYANGINP"
     gene            complement(334..1707)
                     /gene="metG"
                     /locus_tag="PK131_02720"
     CDS             complement(334..1707)
                     /gene="metG"
                     /locus_tag="PK131_02720"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPD45064.1"
                     /translation="MNRFFITTPIYYVNDLPHIGHTCTTVAADILARLHRQLGESVFF
                     LTGTDEHGQKVAEAAAKNNLTPQEYCDQIAPIFENVWKQLKISFDFFIRTTDRRHEQI
                     ATEVLQKIYNNGDIYPGQYEGYYCTGCENFLAESDLVDHHCPLHPAEKTVWKSEKNWF
                     FKLSLYTPKILELIENDQTNYIFPPGKRSEVIAKLKAGVKDISISREKVKWGITTPWD
                     KTQTIYVWIDALINYYSATRFLADKKEFWPADWHLLGKEILWFHTVIWQAMLMSAGIK
                     LPQKTFVHSFYTIDGNKMSKSLGNVISPDQLVRLFGIDGTRYLIARSFPGENDIDVGL
                     EKFKEKYNADLANNLGNLISRVKKLVDNFQSIPLNDQIKFDDEYLKLMTEFKFNEAIQ
                     LVFDKWIDKSNILLNKQTPWKLETTNPIRIKVLEECVDNIQRAIFHLLPVMPGLAEDK
                     SILFPRI"
     gene            complement(1712..1993)
                     /gene="rpsT"
                     /locus_tag="PK131_02725"
     CDS             complement(1712..1993)
                     /gene="rpsT"
                     /locus_tag="PK131_02725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPD45065.1"
                     /translation="MPISRSAKKSWRKSIKNQKINIGWKKRFKEAVKKFKDKPNQTTL
                     NELISFIDKLGQKNIFHHNKVKRLKSKYARQIKIKTPAATKKTKKKVVK"
BASE COUNT          613 a          369 c          375 g          738 t
ORIGIN      
        1 tgggttaata ccgttagcat aatccaaacc acattcgccg atagctttgg cttttttgat
       61 caaatcatcg attttctcaa agggagacat tggatgaatt cccactgccg gaatcaaaaa
      121 atcatatttt tgggccaatt ctaagtttct aaaactttct tttcggctgg ccgccgccaa
      181 aataatccat ttcacctttc cccgtgtttc cggcagaatt ttagccaaat gggcgtgggt
      241 atcaatcatc ttttcatttt agcatgtctg aaattttcca gaatttatcg gctgtagtgc
      301 aattaacaat gaaatttggc taaaattaag gaattaaatt cgcggaaata aaatagattt
      361 gtcttcagct aaacccggca tgaccggtaa taggtggaaa atagccctct gaatattatc
      421 aacacattcc tctaaaactt taattcttat tggattggta gtttctaatt tccatggggt
      481 ctgtttatta agtaaaatat tgcttttatc aatccattta tcaaaaacta attgaatagc
      541 ttcattaaat ttaaattcag tcattaattt taaatattca tcatcaaatt ttatttgatc
      601 atttagagga atagattgaa aattatcgac taattttttg actctcgaga ttaaattgcc
      661 tagattattg gctaaatcgg cgttatattt ttctttaaat ttttcaaggc caacatcaat
      721 atcattttcg cctgggaatg atcgggcgat taaatatcgg gttccatcaa ttccaaaaag
      781 acggactaat tgatccggag aaattacgtt tcccaagctt ttactcattt tattgccatc
      841 gatagtgtaa aaactatgga caaaggtttt ttggggcagt ttaattccag ccgacattaa
      901 catcgcctgc caaataactg tgtggaacca gaggatctcc ttgccaagaa ggtgccagtc
      961 agccggccaa aattcctttt tgtcggctaa aaatctggtg gcgctgtaat aattaatcaa
     1021 agcatcaatc cagacataga tggtctgagt tttgtcccat ggagtggtga taccccattt
     1081 aactttttcc cgagagattg aaatatcttt gaccccggct tttaatttgg ctattacttc
     1141 gcttcttttt cccggcggga aaatgtaatt agtttgatca ttttcgatca attctaagat
     1201 tttaggggta tataaggaca gtttaaaaaa ccagtttttt tctgatttcc aaactgtttt
     1261 ttcagctgga tgaagaggac aatggtgatc gaccaaatct gattcggcta aaaaattttc
     1321 acagccagta caataataac cttcgtattg tccggggtaa atatcaccat tattgtagat
     1381 tttttgaaga acctcagtag ctatttgttc atgtctccga tcggtagttc tgataaagaa
     1441 gtcaaaacta attttgagtt gtttccaaac gttttcgaaa ataggagcaa tttgatcgca
     1501 gtattcctga ggagttaaat tattttttgc tgctgcttcg gctacttttt gtccatgttc
     1561 atcggtgcca gtaagaaaaa aaacagattc acctaactgt cggtgtaatc tggctaaaat
     1621 atctgcggcc actgtggtac aggtatggcc aatatgggga agatcgttaa cataataaat
     1681 aggggtggtg ataaaaaacc ggttcactgg cttattttac tacttttttc ttcgtctttt
     1741 ttgttgccgc tggagtttta attttaattt gacgagcata cttggattta agccgcttaa
     1801 ctttattgtg atgaaaaata tttttttgcc ctaatttatc aataaaagaa attagttcat
     1861 ttaaggttgt ttgatttggc ttatccttaa acttttttac tgcttcttta aacctttttt
     1921 tccaaccgat attaattttt tgatttttaa ttgattttcg ccatgatttt ttggcggagc
     1981 gtgatattgg cataggagat attctaaacg atcgtatata ataaatcaat gcgatggcaa
     2041 gaccagctgt ctaaactttt tcgaactaag caaagttcaa ttatggccgc cagtc
//
LOCUS       DAOOIE010000096         2091 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_42129_length_2091_cov_2.612475, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000096 DAOOIE010000000
VERSION     DAOOIE010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42129_length_2091_cov_2.612475"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="PK131_02730"
     CDS             complement(<1..799)
                     /locus_tag="PK131_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45066.1"
                     /translation="MLKRIYFKLILIGYYLKKSWVYWLVGVSIGFLLYIGRGHLVKIK
                     QLPTFQKQYVGVAGRYTTANLPSEINDLLTYGLTTFTENKKVIASPIVESIYQEDDNH
                     RYIIKLKDNIFWHNGKKLTAADINYNLAGAVIKPEDSLTLMITTPNLFSPLLAALNQP
                     LYKKNLIGLGPNKAIQIDYQGGYLKKLVIQNRDSKIKTVYKFYFSDPDLITAFKLGEV
                     DKIETNLLPTDIEKWNNVNLNQKISPDKKYLAVFLNTEKLNNKSIRQA"
     gene            complement(799..1011)
                     /gene="secG"
                     /locus_tag="PK131_02735"
     CDS             complement(799..1011)
                     /gene="secG"
                     /locus_tag="PK131_02735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPD45067.1"
                     /translation="MKNVLIIAQIITGIALITLIFFQSEGSDESRANILTTTNFEKRG
                     WEKITFQLTIITLVIFLILSIAQTLL"
     gene            complement(1008..1394)
                     /locus_tag="PK131_02740"
     CDS             complement(1008..1394)
                     /locus_tag="PK131_02740"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HPD45068.1"
                     /translation="MKKFLRFTILFYFSLSITNELIHHFYFGQGGLTLLQVSLILTIF
                     ELFLKPIIRILLLPINLLTLGLFRIIIDTFGFYLALLFIDSFQIGTINIWSYHLTGFA
                     AVIITAIITNFIFTIFRSLLTVKIKK"
     gene            complement(1378..>2091)
                     /locus_tag="PK131_02745"
     CDS             complement(1378..>2091)
                     /locus_tag="PK131_02745"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HPD45069.1"
                     /translation="ALQALAEPNQALCLEFTDLFITLARAKGIPAREIEGFAYTNNPK
                     LKPIGLNATILHAWPQYYDNYQQTWIAIDPTWGKTTGGIDYFHDLDLNHFILVIHGRD
                     SLYPPPPDRVEVAFASQEIFPDLKAPLITADRSHLIITNPNPNPLFQINLISPTLEWK
                     NTINFIGPYGKTSINLPKMSFIDSLRRHNQTIDIFYENQKYNLKYLPHYQNLALTVTA
                     AIVLLCLCGIMITRHEKIS"
BASE COUNT          707 a          286 c          465 g          633 t
ORIGIN      
        1 aggcctggcg gatcgattta ttgttgagtt tttcagtatt taagaaaaca gccaaatatt
       61 ttttatcggg agaaattttt tggttgagat taacattatt ccatttttca atatcggtcg
      121 gcaaaaggtt ggtttcaatt ttatccacct ctcctaattt aaaagcggtg attaaatcag
      181 gatcggaaaa gtaaaattta taaacagttt taattttact atctctattt tgaataacaa
      241 gtttttttaa ataaccaccc tgataatcaa tttggattgc tttattagga ccaaggccga
      301 tcaaattctt tttataaagc ggttgattta aggcggctag taagggggaa aataaatttg
      361 gggtagtaat cattaaagtt aaactatctt cgggtttaat aacggcgcca gccagattat
      421 agttaatgtc ggcggcggtt agttttttac cattgtgcca aaaaatatta tccttaagtt
      481 taatgatata acggtgatta tcgtcttctt ggtaaattga ttcgactatg ggagaagcga
      541 tcactttttt attttcggta aaagtggtta gaccgtaagt aagaagatcg ttaatttccg
      601 aaggcagatt agcagtggtg tacctgccgg cgacgccaac atattgtttt tggaaagttg
      661 gtaattgttt gattttaacc aaatggccac ggccgatata taaaagaaaa cctatagaca
      721 ctccgactaa ccaataaacc caactttttt tgagatagta gccgattagg attaatttaa
      781 aatagattct tttgagcatt agaggagagt ttgagcgata gagagaatga ggaaaatgac
      841 gagagtgata atcgttagtt gaaaggtaat tttctcccaa ccccgtttct caaagttagt
      901 ggtggtgaga atattagctc gggattcgtc actaccttcg gattgaaaaa aaatcaaggt
      961 aattagagca ataccggtaa taatttgagc gataataaga acgtttttca ttttttaatt
     1021 ttgacagtta aaagggagcg aaaaatagta aaaataaagt tggtaataat ggccgtaata
     1081 atcacagccg caaaaccggt tagatggtag gaccagatat taattgttcc gatctgaaaa
     1141 gaatcaataa aaagaagagc taaataaaaa ccgaaagtat caataataat tctaaataga
     1201 cccaaggtca ggaggttgat tggcagaagg agaattctaa tgatgggttt aaggaaaagt
     1261 tcgaaaatag tcagaattaa actgacttgg agaagagtta agccaccctg gccaaaataa
     1321 aagtgatgga ttaattcgtt agtaatagat aatgagaagt agaaaagaat ggtaaaccta
     1381 agaaattttt tcatgtctgg ttatcattat accgcaaaga caaagaagaa cgatggccgc
     1441 ggtaactgtc aaggctagat tttggtaatg aggcaggtat tttaagttgt atttctggtt
     1501 ttcataaaaa atatcgatgg tttgattgtg tcgacggaga gagtcgataa aggacatttt
     1561 cgggagattt atggaggttt ttccgtaagg tccaatgaaa ttaatagtat ttttccactc
     1621 aagagtcggt gagattaagt taatttggaa aagaggatta ggattaggat tggtaatgat
     1681 taaatgagat cgatcggcgg taattaaagg agccttgaga tcgggaaaaa tttcttgact
     1741 ggcaaaagct acttcaactc gatcaggagg cggtggataa aggctatccc gaccatggat
     1801 aactaaaata aaatgattaa gatctaaatc gtggaaatag tcaataccac cagtagtttt
     1861 accccaagtg ggatcaatag caatccatgt ttgttggtag ttgtcgtaat attgcggcca
     1921 ggcgtgcaaa atagtagcgt taagaccgat gggtttaagt tttggattgt tagtgtaagc
     1981 aaagccttca atttctcggg ctggaattcc tttagctcga gctaaagtga taaatagatc
     2041 agtaaattca agacaaagag cttggttagg ttcagctaaa gcttggagag c
//
LOCUS       DAOOIE010000097         2062 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_42969_length_2062_cov_3.040857, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000097 DAOOIE010000000
VERSION     DAOOIE010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42969_length_2062_cov_3.040857"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="PK131_02750"
     CDS             complement(<1..270)
                     /locus_tag="PK131_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45070.1"
                     /translation="MAVEKSNNLLIALRFIFGWIIISVYLLITLVAKHIPLAMLPMTL
                     LTPPVTLIFLPPLIGAGYFMFSSRMNWRVDFTMLLITILCLIGIAN"
     gene            320..484
                     /locus_tag="PK131_02755"
     CDS             320..484
                     /locus_tag="PK131_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45071.1"
                     /translation="MGNLNKSYLIAANAGSCFVLPHPSPLNIKWFKNNPQFEREKLTQ
                     IRAAIKKIVL"
     gene            481..996
                     /locus_tag="PK131_02760"
     CDS             481..996
                     /locus_tag="PK131_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013194225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HPD45072.1"
                     /translation="MKKLFLTVGIIIITAIFLFGCGKKAAMRLTSPVFNNNDFLPDKY
                     ACRGANVNPPLVIDDVPAGTQSLVLIVDDPDAPAGTWTHWLVWNIKPEITEIAENSVP
                     AGAVEGTTSWGKPGYDGPCPPSGTHRYFFKVFALNCEISLSSEADINRLNQTMKDHII
                     AKAEIIAKYKK"
     gene            1204..>2062
                     /locus_tag="PK131_02765"
     CDS             1204..>2062
                     /locus_tag="PK131_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HPD45073.1"
                     /translation="MCGIFGYVSRRPVATGVVFTGLKELEYRGYDSWGIVWETKKGLG
                     ISKHLGKMDQKPDRLPKSSISLGHTRWATHGGITFDNCHPHFDCTGNIAVVHNGIIEN
                     YQELKAELIKKGHRFTSETDSEVIAHLVEELGSVKSAFKKLKGANAISVLIHGRQEIV
                     IAKAGSPLVVGLGQGINYVASDPNPLLPYTNKFIFLDDGEVLKINSQEIKSERIKIIQ
                     PPAENLSSSLGKYPNYLIKEISEQPKILKIINDNYDLSPLRKIFKLIKSKDRVFFTGC
                     GTAYFAALFS"
BASE COUNT          629 a          362 c          458 g          613 t
ORIGIN      
        1 gttggcgata ccaattaaac agagaatagt aattagtagc atcgtaaaat caacccgcca
       61 attcattctg gaggaaaaca taaagtaacc ggcgccaatt aatggcggca ggaaaataag
      121 agttaccggc ggggtcaaca atgtcattgg cagcatggct aggggaatgt gtttagcaac
      181 gagggttatg aggagataaa cgctgatgat aatccagccg aagataaatc ggagagcaat
      241 caaaagatta ttggattttt ctaccgccat aattaattat agccttttta acggcaatga
      301 taaaattagg ttatggattt tgggcaattt aaacaagagt tatttaattg ccgccaatgc
      361 cggcagctgt ttcgttttgc cccatccatc gccgttaaat attaaatggt tcaagaataa
      421 ccctcaattt gagagggaaa aattgactca aattcgagcg gcgattaaaa aaattgtttt
      481 atgaaaaagc tttttttaac tgtcggcatt ataattatta cggccatatt tttatttggc
      541 tgtggtaaaa aagcggctat gcggctgaca agtcctgttt ttaataataa tgattttctt
      601 cctgataagt atgcctgccg aggggccaat gttaatccgc cgttggtaat tgatgatgtt
      661 ccggccggga cccaatccct ggttttgatt gtcgatgatc ctgacgctcc ggctggtact
      721 tggactcatt ggctggtttg gaatattaag ccggagataa cagaaattgc cgaaaactcg
      781 gtgccggcag gggcagttga gggcactacc agctggggca agccgggtta tgacggccct
      841 tgtccgcctt cgggaaccca tcgctatttt ttcaaagttt ttgccttaaa ttgtgagatt
      901 agtctttcat ctgaggctga tattaatcga ctaaaccaaa ctatgaagga ccatattatt
      961 gccaaagccg aaattatagc taaatataaa aagtaatttt cagagtataa tcgtttcagg
     1021 atggcgctct ttattaacca atagcgtgtg gtccgccaac tggcggaaga cgaggaagag
     1081 ggtgatcgaa tcatctgcgg atgccctcag gttaggccgt taacctaaaa agctttttga
     1141 aaagaaaagt ttggacggca gcgttatccc attattattt atcctaatat agggggtatt
     1201 aatatgtgtg ggattttcgg ctatgtcagc cggcggccgg tggctaccgg ggtggttttt
     1261 actggtttaa aagaattaga atatcggggc tatgacagtt ggggaatagt ttgggagact
     1321 aaaaaaggtt tgggaattag caaacatttg ggaaaaatgg atcaaaagcc cgatcgattg
     1381 cctaaaagtt caatatcatt aggccatacc cgttgggcaa ctcacggcgg catcactttt
     1441 gacaattgtc atccccattt tgactgcacc ggtaatattg ccgtagttca taacggcatt
     1501 attgaaaatt atcaagaact taaggcagaa ttaattaaaa aaggtcatcg attcacctcc
     1561 gaaacagact ctgaagttat tgctcattta gtggaagaat taggttcagt taaatcagct
     1621 tttaaaaagt taaaaggggc caacgctata tccgttttaa ttcatggtcg acaggagatt
     1681 gttattgcta aagccggctc gccactggta gttggtttgg gtcaggggat taattatgtt
     1741 gcctctgatc ctaacccact tcttccttat actaataaat ttattttttt agatgacggt
     1801 gaggttttaa aaattaattc tcaggaaata aaaagtgaga gaataaaaat aatccaacca
     1861 ccggctgaaa atcttagttc gtctttggga aaatatccta attatttaat taaagaaatt
     1921 tccgaacaac caaagatttt gaaaattatt aatgataatt atgatctttc tcctctccga
     1981 aaaatattta aattaataaa aagtaaggac agagtatttt ttactggttg tggcaccgct
     2041 tattttgccg ccttatttag cg
//
LOCUS       DAOOIE010000098         2055 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_43175_length_2055_cov_3.249000, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000098 DAOOIE010000000
VERSION     DAOOIE010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43175_length_2055_cov_3.249000"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="PK131_02770"
     CDS             <1..756
                     /locus_tag="PK131_02770"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HPD45074.1"
                     /translation="GYICGYFGWSNLERRDLEKEGITRAEQLIDCQQNILVAVDKSFL
                     PGVIGLIAGRLTEKYYLPTIIIAEDKDISKASCRSIPEINIIKLLRNFTDLLLDLGGH
                     AQAAGFSILTKNIPKFIHQIEKYPLKKFNRPIQAEARMLLSAVNMRNIRAIERLAPFG
                     LGNPKPVFLFEKAVITGKRMLGSDHNHLKLILNDSVKAIAFRRKDLDSALNMGDIINL
                     TASVETDDYGGTASPQLIIKEIFKSNDGGSFKK"
     gene            731..>2055
                     /locus_tag="PK131_02775"
     CDS             731..>2055
                     /locus_tag="PK131_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45075.1"
                     /translation="MTAEVLKNESVLTKLDRDINLLLKQGFRRREVVNQLTDVPTNIS
                     RKTLERIIKEADLSIKTPQVVDLIRRHLPLRQVVKETSLPYKDIHQIIRTNLEPEEYR
                     VIVESNLINRYKEKPLPSPEQLKPYLVDFRTPGYIGRQIGMSSYMVEKGLRKLLGEEY
                     SDYQKKAARLGHYLLDKPVSYYLTQIESLIEQKYTHVQILKELSLGYFAFKFIYELKQ
                     GNNYHEYLTKINRENKLFQHYREYLPQIKQYLKEGKSPSFIENKLKITQGRLGLIMEK
                     WLDEDIGRKDDFIRDLPIITDKLESHQLKMTKLICHYRLSRSTIENLLLKEKGEKWGE
                     KYLPSRSNCSSFDQNQIALVSHLSKVTEQLETGMTTMTKLVRDWQLSKNAIQKILEEK
                     NGQKWYETYVTAHRGRHENYNMRKRKNFIESLPQVVKQIEEKKINLAVLS"
BASE COUNT          721 a          315 c          406 g          613 t
ORIGIN      
        1 ggctatatat gcggctattt tggatggtcg aatttagaac ggcgggattt ggaaaaagaa
       61 ggcattacta gggcagaaca actcattgat tgtcagcaga atattttagt agcggttgat
      121 aagtcatttt taccgggagt tattggctta atcgctggta gattaactga aaaatattat
      181 ctccctacaa taattattgc cgaagataag gatatttcga aggcatcatg tcgttctatt
      241 ccggaaatca atataattaa attgttaagg aattttaccg acctgctttt ggatttaggc
      301 ggccatgctc aagcagctgg tttttcgatt ttaactaaaa atattcctaa atttatccac
      361 caaattgaaa aatatcccct taaaaaattt aatcggccaa ttcaggctga ggccagaatg
      421 cttctctctg cggttaatat gcggaatata agggcgattg agaggctggc tccttttggc
      481 ctcggtaatc ccaaaccagt atttttgttt gaaaaagccg taattacagg taaaagaatg
      541 ttagggtctg accataatca tttaaaacta attttgaatg attcagttaa agcgatagct
      601 ttcagaagaa aagatcttga ttcagcctta aacatgggtg atataataaa cttaactgcc
      661 tctgtggaaa ctgatgatta tgggggtacc gcctcgcccc aactaataat taaagaaatt
      721 tttaaatcaa atgacggcgg aagttttaaa aaatgaatca gttttaacca agttagatag
      781 ggatattaat ctacttttaa agcagggttt tagacgaaga gaagttgtta atcaattgac
      841 tgatgttccg actaatattt cgagaaagac tttagaacgg attattaaag aggctgatct
      901 ttcaataaaa actccacagg tagttgatct gattagacgt caccttcctc tccgtcaagt
      961 agtgaaggaa acgtctttgc catataagga tattcatcag attattagaa ctaatcttga
     1021 accggaggaa tatcgggtta ttgtggaatc gaatctaatt aatcgttata aagaaaagcc
     1081 gttaccgtca cccgaacaat taaagccata tctagtggat tttcgaacgc caggatatat
     1141 tggtagacaa atcggtatga gtagttatat ggtagaaaaa gggctaagaa aattactggg
     1201 tgaagaatat agcgactatc aaaaaaaagc cgctaggtta ggtcattacc tattggataa
     1261 gccagtatct tattatttaa cccaaattga atccttaatt gaacagaaat atactcatgt
     1321 tcagatctta aaagaattat ccttaggtta ctttgcattt aaatttattt acgagctaaa
     1381 acagggtaat aattatcatg agtatctgac taaaattaat cgtgaaaaca aattgtttca
     1441 acactatcgg gagtatttac cccaaatcaa acaatatcta aaagagggta aaagtccgtc
     1501 ttttattgag aataaactga aaattactca gggccgtttg gggttgataa tggaaaaatg
     1561 gctagatgaa gatatcggcc gtaaggatga ttttattcgt gatttgccaa ttataaccga
     1621 caaattagaa tctcatcaat taaaaatgac aaaattaatt tgtcattatc gactgtctcg
     1681 gtcaactatc gagaaccttc ttttgaagga aaaaggggaa aaatggggtg agaagtattt
     1741 accttcgcga tcaaattgtt cttcttttga tcaaaaccaa attgctttag ttagtcatct
     1801 atcaaaggta actgaacagt tagaaacagg gatgactacg atgacaaaat tagttagaga
     1861 ctggcaatta agtaagaatg ctatccagaa aatattggaa gaaaaaaatg gtcaaaagtg
     1921 gtatgaaact tacgtgaccg cccaccgcgg ccgtcatgag aattataata tgagaaagcg
     1981 gaaaaatttt attgaaagtt tacctcaggt agtaaaacaa attgaggaga aaaagataaa
     2041 tctggctgtt ttatc
//
LOCUS       DAOOIE010000099         2053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_43248_length_2053_cov_2.880380, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000099 DAOOIE010000000
VERSION     DAOOIE010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43248_length_2053_cov_2.880380"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="PK131_02780"
     CDS             complement(<1..861)
                     /locus_tag="PK131_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD45076.1"
                     /translation="MDKIKELVRQLTEKVAAAHLPVGGEEKLKEVLTEAELDLHNFPL
                     TSRYVNWFLTLPWHKSSQDILDLTYAKEQLDKYHYGLTEIKQRILEYISVLALQKQRG
                     NLTFSRAPIFLFVGLVGTGKTTLAESIAAALGRNYVRIPFGGLGDPFYLRGHSRFDSE
                     AEPGAIIKALKTVQTNNPVILLDEIDRIADPAFSSIMGTLVELLDPEQNDHFTDSYLD
                     YPFDLSQVIFCATCNNTNRLATAVLDRLEPLMMPAYTDEEKTVIGRDYLLPKVLSGSG
                     LTSQDLVISPS"
     gene            complement(913..>2053)
                     /locus_tag="PK131_02785"
     CDS             complement(913..>2053)
                     /locus_tag="PK131_02785"
                     /inference="COORDINATES: protein motif:HMM:NF013318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPD45077.1"
                     /translation="TLSTLADRQYLQDSSGETTKRYIHYYSAMPFSTGETGRVGRPGR
                     REIGHGALAEKALMPVIPSEEEFPYLIMLTSEILSQNGSSSMASTCGSTLSLLDAGVP
                     IKDKVAGISIGMVAESENNYLLLTDIAGIEDHYGDMDFKIAGTRGGITAIQLDVKCRG
                     LTLPMIKETFTRSLQARLQILDLIDKTMPAPRQELSPYAPKIEVVVIPEDKIGDVIGS
                     GGKTIRALMEKYGVQIDIEDNGQAYIAGPDEVKVKAAAFEISSMVREIRTGEEFNGQV
                     TRVEDYGVFVEFLPGREALLHISEMSQGFISDPRKIIKIGDKLHVKVAGFNENHQIRL
                     AAPEFKAAHQGEAMPNRRQAMPTGRQAQSFSHFHPNLRPQRNRRP"
BASE COUNT          623 a          492 c          389 g          549 t
ORIGIN      
        1 agatggacta atcaccaaat cttgactggt caaaccactt cctgataaaa ctttaggtaa
       61 caaataatcc cggccaatta cggttttttc ttcatcagta taagctggca tcattaacgg
      121 ctccagccga tctaaaacag ctgttgccaa ccgattggtg ttattacagg tggcacaaaa
      181 aatcacttgg gataaatcaa aaggataatc aagataacta tcggtaaaat gatcattttg
      241 ttcaggatcg agtaattcaa ctaaagttcc cataattgaa ctgaaagccg gatcggctat
      301 tcgatcaatt tcatccagta aaataaccgg attattagtt tgaactgttt ttaaggcttt
      361 aataatagct ccgggttcag cttcggaatc aaagcgggaa tggcctcgga ggtaaaaagg
      421 atcgcctaag ccgccaaatg ggatccggac atagttccga ccaagagcgg cggcaatact
      481 ctcggctaaa gttgtcttgc cggttccaac taaaccgacg aaaagaaaaa tcggcgcccg
      541 ggaaaaagtt aaattacccc gctgtttctg cagggccagc accgaaatat attctaaaat
      601 gcgctgttta atttccgtca agccataatg gtatttatct aattgctcct tagcataggt
      661 taaatctaaa atatcctgac ttgatttatg ccaaggaaga gtcaaaaacc aattaacata
      721 gcggctagtt aaggggaaat tatgaagatc caattccgct tcagttaaaa cctcttttaa
      781 cttttcttcc cctccaaccg gtaaatgagc tgctgccact ttttccgtta gctgacgaac
      841 caattcctta atcttgtcca ttataatcag tataccctac tatccccgct caggtgataa
      901 attaaatcct gcttatggcc gccgattccg ttgtgggcgg aggttgggat gaaaatggct
      961 aaaagactgg gcctgcctgc cagtaggcat agcctgcctg cgattaggca tagcctcacc
     1021 ctgatgggcc gccttaaatt ctggagctgc caaacgaatc tgatgatttt cattaaaacc
     1081 agccacttta acatgcaact tatcgcctat tttgataatt ttccgcgggt cggaaataaa
     1141 accctgactc atttcggaaa tatgcagtaa agcctcacgc ccaggtaaaa actcaacaaa
     1201 aactccataa tcctcaactc gggtaacctg gccgttaaat tcctccccgg ttcgaatttc
     1261 cctaaccatc gaacttattt caaaagcagc cgccttcacc ttaacctcat cagggccggc
     1321 gatataggct tgaccattat cttcaatatc gatttgcact ccatatttct ccattaaagc
     1381 ccggatagtc ttaccgccag aaccaataac atcaccaatt ttatcttcag gaataactac
     1441 aacttcgatt ttaggagcat agggtgataa ttcctgtctc ggggcgggca tagttttatc
     1501 gattaaatca agaatctgca atctggcttg taaacttctg gtaaaagttt ccttaatcat
     1561 cggcaatgtc aaaccccggc atttaacatc caattgaatc gcggtaattc ctccgcgggt
     1621 accggcaatc ttaaaatcca tgtcaccata atgatcttca atcccggcaa tatcagtcaa
     1681 aagtaaatag ttattttcac tttcggctac cataccgatc gaaataccag caactttatc
     1741 tttaatcggc acgccggcgt ctaaaagcga taaggttgaa ccacaggtag aagccatcga
     1801 ggaagacccg ttttgagaca aaatctcaga agtcagcata atcaaataag ggaattcttc
     1861 ttcagaggga ataaccggca ttaaggcctt ttcagccaaa gctccatgac cgatttcccg
     1921 acgacctggg cggccaaccc gacctgtttc accagttgaa aaaggcatcg ccgaataata
     1981 gtgaatataa cgcttagttg tttcaccact gctatcctgc agatactgtc tatcagccaa
     2041 agtcgacaaa gta
//
LOCUS       DAOOIE010000100         2016 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_44408_length_2016_cov_1.818970, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000100 DAOOIE010000000
VERSION     DAOOIE010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44408_length_2016_cov_1.818970"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(140..1579)
                     /locus_tag="PK131_02790"
     CDS             complement(140..1579)
                     /locus_tag="PK131_02790"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HPD45078.1"
                     /translation="MVKFDPLRLTVCFLTGLALASFWLIDWLIIFLLISLGLIILIID
                     RRSSMAVFFIGLALGAGYLNLQVEKRLAIQERWQAEAEVVLVGTVVTRPELRPTGAHL
                     EVKLDWPVLARSPELRGHFTVFGDYAWAEFSPGQRLVVSGRPTISEEFQNYRLKEGVY
                     ATFFHPKVELIGRNNNYFIPYLASRVQDGASSLIDQRLAEPSASLLKAMLLGQRISNS
                     SFSDHLSRTGLNHIAVVSGMHLVVLAGILRSLFKKLKFSQPGSAKLILVAILIFVLLT
                     GGRPSIWRAGLMVGLGFLGPILGRLYQPGRAIIWAAFFILLANPLLLRWDIGFQLSFL
                     AATGLICWFDQLKKWLSWVPVTLLRESLAVTLAAQLATIPLSAYYFQQLSLVGPLANI
                     AVVIFLPLIFTLGLAFLLSAGFTPVAWLTGLVLAVITQMIVFFSGWGWSAIVLTVPVW
                     FLVIYYFAWFLASLKNFQATSAFHAERLC"
     gene            complement(1579..>2016)
                     /locus_tag="PK131_02795"
     CDS             complement(1579..>2016)
                     /locus_tag="PK131_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HPD45079.1"
                     /translation="KIKKLGFLVKIDTNGSHPEVLAELLKQSLVDYWAMDIKAPQGKY
                     SAIAQTEIDFRLIQQSIDFIMNSGQAYEFRTTVVRNQLVPEDIMEIGQMIRGAEKYVL
                     QPFVSAGKTVDPKFLSAQSYSQEEMRSWAQELEANFVKHCLVR"
BASE COUNT          634 a          517 c          442 g          423 t
ORIGIN      
        1 ttgattgtag ttaacgacta aatagataga aaccccggcc aagatcacaa ccaaaactcc
       61 ggccactact tcaactaggg taattctaat ttttttcttg gtaggtgaag acataagcct
      121 taccgtcatc tagttcattt taacacaatc tttcagcatg aaaagctgaa gttgcttgaa
      181 aatttttcag gctggctaaa aaccaggcaa agtaatagat tactaaaaac caaaccggga
      241 cagttaaaac aatggcggac cagccccaac cggaaaaaaa gacaatcatt tgagtaatga
      301 ctgctaaaac cagccccgtt aaccaggcga ccggggtaaa tccagctgac agcaggaagg
      361 ccaagcctaa ggtgaaaatc aagggcaaga agatcaccac cgcgatatta gccagcggcc
      421 cgaccaagga caattgctga aagtaatagg ccgaaagagg aatcgtcgct agctgcgccg
      481 ccaaagtaac ggccagagat tcccgcagca gggtaaccgg gacccagctc aaccatttct
      541 ttagctgatc aaaccagcaa attaaaccgg tggcggccaa aaaagaaagc tgaaagccga
      601 tgtcccatcg tagcagcagc ggattagcta acaaaataaa gaaagcggcc cagataatag
      661 cccgacccgg ttgataaaga cggcctaaaa taggccccag gaagcccaag cccaccatta
      721 acccggctcg ccagatcgag ggacggccac cggttaataa cacaaagatt aagattgcca
      781 ccaaaattag cttagccgag cccggctggc taaatttaag cttcttaaag aggctcctta
      841 aaattccagc caaaacaacc agatgcattc cggaaaccac tgcaatgtgg ttcaggccag
      901 tccgggacag gtgatcggaa aaagatgaat tagaaatcct ctgaccgagc aacatcgctt
      961 tcaagagaga agctgaaggc tcagccagcc gctggtcgat caaggacgat gccccatctt
     1021 gaacccgaga cgctaaataa gggataaaat aattattatt gcgaccgatc aattcaacct
     1081 ttggatgaaa aaaggtggca taaacgccct ccttgagacg ataattttga aattcctccg
     1141 agatagtcgg ccggccagag accaccaagc gctggcctgg agaaaattca gcccaggcat
     1201 agtctccaaa aacagtgaag tgtcccctta gttccggaga acgagctaaa accggccagt
     1261 ctaatttaac ctccaggtgg gctcccgttg gacgaagctc cggacgggta acaactgtgc
     1321 ccaccaaaac cacctctgcc tcggcctgcc atctttcctg aatcgccagg cgtttctcca
     1381 cttgcaggtt tagatagccc gcccccaagg ctagcccaat gaaaaagacc gccatgctgg
     1441 agcggcggtc gatgatcaga ataattaacc caagagagat caataggaag ataattagcc
     1501 agtcaatcaa ccagaatgag gccagcgcca acccggtcaa gaaacagaca gttaaacgga
     1561 gaggatcgaa tttgaccatc agcgaaccaa acagtgctta acgaagtttg cctcaagctc
     1621 ttgagcccac gatctcatct cttcttgaga atagctttga gccgaaagaa atttcggatc
     1681 aactgtcttc ccggcggaaa cgaatggctg gagaacatac ttttcagccc cccgaatcat
     1741 ctggccaatt tccataatgt cttctgggac cagctgattt ctgacaaccg ttgttcgaaa
     1801 ctcataggcc tgtcctgaat tcataatgaa atcaatgctt tgttgaatta atctaaaatc
     1861 aatctcagtc tgagcgattg ccgaatattt tccctggggt gccttaatgt ccattgccca
     1921 gtaatcaact aagctctgct ttaacagctc agccaacacc tccgggtggc tgccgttggt
     1981 atctatcttg actaaaaagc ccagcttttt aatttt
//
LOCUS       DAOOIE010000101         1997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_45047_length_1997_cov_2.944902, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000101 DAOOIE010000000
VERSION     DAOOIE010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45047_length_1997_cov_2.944902"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PK131_02800"
     CDS             complement(<1..638)
                     /locus_tag="PK131_02800"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPD45080.1"
                     /translation="MGKENQQGFILVDSPEQRQTELQAEMQHIWPLKDEMYSLDKFLE
                     DYLLLEDSKITPSKREMAAIRVKRAINQIRLLDSGHDKSLLFGYLVEEYCHNSEPTAT
                     YGIFLPTYVEAMKDKKIDKALLLGTYNIRSLIEFNAGVKYLYPQAECVVIDKNPAEQV
                     KDYRHFIEGDILDMPFAANTFDSIQSNFLFTMLEGEKEKAFAEVTRVLKPNG"
     gene            complement(672..1778)
                     /gene="metK"
                     /locus_tag="PK131_02805"
     CDS             complement(672..1778)
                     /gene="metK"
                     /locus_tag="PK131_02805"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPD45081.1"
                     /translation="MNYSLFTSESVCSGHPDKICDQISDAVLDAALTVDPQSRVALET
                     MATENYLTLAGEVTCAQKLDYKSIARGVIKDLGYNRPIYNFTYRSPIDIRVHHQSVDI
                     ALGVDGGGAGDQGMMFGYAVAETKEFMPLPISIAHDLAREIDRIRETKLTYLRPDGKS
                     EVSVRYENGLPVAIEALVIAVPYDPQMAREEVKEALYREVVLPVLDKYQQKPISLDKM
                     IFNGTGQWEIGGPASDTGVTGRKIIVDTYGGMGRIGGGSFSGKDPSKVDRSAAYAARF
                     IAKNIVAHGLAHRCEVQLAYAIGSRQPISRSIETFGTGKKSQKVIETFAWNLLDLSPQ
                     GIIKALNLRRPIYRSTAVYGHFGNPSYPWEKIVS"
BASE COUNT          595 a          459 c          371 g          572 t
ORIGIN      
        1 tggccgtttg gtttcaaaac tcgggttact tcggcaaacg ccttttcttt ttctccttct
       61 aacatagtaa acaaaaaatt agattggata ctatcaaaag tattggcggc aaaaggcata
      121 tctaaaatat caccctcaat aaaatgacgg tagtccttga cctgctctgc aggattttta
      181 tcaataacaa cacattcagc ttggggatat aaatatttaa ccccggcatt aaattcaatt
      241 aaagaacgga tattatatgt ccctaataaa agggctttat cgattttttt atctttcatg
      301 gcttccacat aagttggcaa aaagatgcca taggtagctg ttggttcaga attatgacaa
      361 tattcctcaa ctaaatagcc aaaaagtagg cttttatcat gtcccgaatc taatagtcta
      421 atttggttaa tcgccctttt tacccgaata gctgccattt cccgtttcga aggagtaatt
      481 ttgctgtctt cgaggagtaa ataatcttcc agaaatttat ctaacgaata catctcatct
      541 ttcaacggcc aaatatgttg catttctgcc tggagttcag tctgccgctg ttccggacta
      601 tccaccagaa taaaaccctg ctgattttct tttcccataa cttctaatta tatcatatta
      661 taggccgaat gtcaagaaac aattttttcc caaggatagg aaggattgcc gaaatgacca
      721 taaacagccg tcgatcggta gatagggcga cgtaaattca aggctttgat aattccctga
      781 ggtgataaat ccaataaatt ccaagcaaaa gtctcgatta ctttttgaga ttttttgccg
      841 gtaccgaaag tttctatcga ccgactaatc ggctgacggc taccgatagc ataggccaac
      901 tgaacttcac agcggtgagc caaaccatga gccactatat ttttagcaat aaagcgagcg
      961 gcgtaggctg ccgaccgatc aactttactt ggatctttac cagaaaaaga accaccgcca
     1021 attcgcccca tcccgccgta ggtatcgaca ataattttcc ggccggttac tccggtatca
     1081 gaggccgggc cgccgatttc ccactgacca gtaccattaa aaatcatttt atctaatgaa
     1141 atcggtttct gttggtattt atctaaaact ggtaaaacta cctcccgata aagagcttct
     1201 tttacctcct cccgagccat ttggggatca taaggaacag cgatcactaa agcctcgata
     1261 gctaccggca aaccattttc atatctgaca ctgacttcgg acttgccatc ggggcgaaga
     1321 taggtcaatt ttgtctctct aatccggtca atttctcgag ctaaatcgtg ggcaatcgaa
     1381 atgggtaaag gcataaattc tttagtctcg gctaccgcat aaccaaacat cattccctgg
     1441 tcaccagcac cgccgccatc aaccccaaga gcaatatcaa ccgactgatg atgaacccga
     1501 atatcaatcg gcgatcgata agtaaaattg taaatcggcc gattataacc caaatcttta
     1561 attactcctc gggcaattga cttataatcc agcttttggg cacaagttac ttctccggct
     1621 aatgtcaaat aattttcggt ggccatagtc tccaaggcta ctcgtgactg agggtcaact
     1681 gttaatgccg catcaagaac ggcatcagaa atttgatcac aaattttgtc aggatgacca
     1741 gaacaaactg attcggatgt aaaaagggaa tagttcattt ttcctccttt taacctttaa
     1801 tagaggcctg tactaaagca ccgtgaccca ctaactagcg gactcggttg ctacctcgac
     1861 agttgccggg gttcttgaca gccaaataga ttatagcatt accattacct aatttgaaaa
     1921 cctcgataaa cgatctttaa gggcttgata attaattgtt ttggaccctt gttaacagtg
     1981 ccggcgatcc aagaatg
//
LOCUS       DAOOIE010000102         1995 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_45110_length_1995_cov_3.853093, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000102 DAOOIE010000000
VERSION     DAOOIE010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45110_length_1995_cov_3.853093"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..>1995)
                     /locus_tag="PK131_02810"
     CDS             complement(191..>1995)
                     /locus_tag="PK131_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HPD45082.1"
                     /translation="FPTAGEIHGRRGIIEMYTTGRGKFDVRGKAEIETTKSGKNRIVI
                     TELPYQVNKAELVKKIADLVRDKKIIGIADLRDESDRHGIRLVVEIKKNGKSKAILNK
                     LYKYTTLQSSYPANMLALVDGVPQTLNLRQMLLLYLRHREEIVRRRTIFDLKSASLRA
                     HILAGLKKALDNIDEIIKTIKTSKNTDDARNNLMKKFDFTDLQANAILEMQLRRLAAL
                     ERQKIEDEFTEITQQINDLTKIITEPAKMISLLKDETAEITNKYGDDRKTRIYVRGLE
                     DFSDEDLIPNEPVLITITKTGYIKRVPKDTYHSQKRGGVGVVGMTTKIEDEIESILAV
                     DTHDDLLFFTNKGRVLKTKVWEVAEGNRQAKGQAIVNLINVIHGESVQAVLAINKNKP
                     AQYIMLATRQGIVKKTSLDKFKNIRQSGLTAIKLDGGDSLIFAKTTSGSDQIFLVSHN
                     GKCIRFNETDCRPMGRHTRGVRGILLKGDDHLVSMEVIPAISTTFQHLLVVTENGIGK
                     RSNVYLYPLQKRGGIGVKVANITAKTGKIAAAQVVTDKHDQVILISKKAVTIKLPLKN
                     IPELNRNTQGVILMRFKTNDDKFNALTVLEKNIG"
BASE COUNT          554 a          397 c          379 g          665 t
ORIGIN      
        1 tggaaagtaa ggaaaaaata taaataatcg ggtgatttat taacatattg gatatgtata
       61 tatatttttt cccctattat agggttaaag tagaaattac agcctgatta gcagatagtt
      121 attttgactg ctaatcccct cccccattct gcacaagacc tatattgttt agtataagac
      181 atgatttttg ctaccctata tttttttcga ggacagttaa ggcattaaat ttgtcatcat
      241 tggttttaaa gcgcattaaa ataacccctt gagtattgcg atttaattca ggaatatttt
      301 ttaggggtaa tttaatagtt actgcctttt tggaaataag aataacctga tcatgtttat
      361 cagttactac ttgagccgcc gcaattttac ccgttttggc ggtaatattg gcgaccttga
      421 cgccaatgcc tcctcgtttt tgtaaaggat aaaggtaaac attactcctt ttaccaatgc
      481 cgttttcagt taccaccaat aaatgttgga aagtagtgga aatagctgga ataacttcca
      541 tactaactag atggtcatct ccttttaaca aaatgccgcg gacgccacgg gtatgacgtc
      601 ccatcggccg gcagtcggtt tcgttaaaac ggatacattt accattatgg ctaactagaa
      661 aaatttggtc ggatccactg gtagttttgg caaaaataag actatctccc ccatcgagct
      721 taatcgcggt caagccgctt tggcggatat ttttaaactt atctagactg gtttttttaa
      781 cgataccctg gcgagtagcc agcataatat attgagctgg tttgttctta tttatggcta
      841 aaactgcttg aacactttca ccgtggataa cattaatcaa attaacaata gcttgtcctt
      901 ttgcttgtcg attgccttca gctacctccc aaactttagt ttttaaaacc cggcctttat
      961 tagtaaagaa aagaaggtca tcatgagtgt caacggccaa aattgattcg atttcatctt
     1021 caatcttagt tgtcatacct accaccccta ccccaccccg cttttggctg tggtaggtgt
     1081 ctttcggaac ccgtttaata taaccggtct tagtaatagt gattaacact ggttcattag
     1141 gaattaaatc ctcatcagaa aagtcttcca gacctctgac atagatcctg gtttttcggt
     1201 catcaccata tttattggta atttcggcag tttcatcttt taagagcgaa atcatcttgg
     1261 ctggttcagt gataattttg gttaaatcgt taatttgttg agtaatttca gtaaattcgt
     1321 cttcgatttt ttgccgctcc aaagctgcca gccgccgcag ctgcatttcc aagatagcat
     1381 tggcctgtaa atcggtgaag tcaaactttt tcattaaatt atttcgggcg tcatcagtat
     1441 ttttagaagt tttgattgtt ttaataattt catcgatatt atcaagagct tttttaagac
     1501 ctgccaaaat gtgggctcgt aacgaggctg acttgaggtc aaaaatggtt cgccggcgga
     1561 cgatttcttc ccgatgacga agataaagaa gaagcatttg gcggagattt aaagtttgag
     1621 gcacaccatc gaccaaggcc agcatattag ctggataaga tgattgtaag gtagtgtatt
     1681 tataaagttt attcagaatt gctttggatt tgccgttttt cttaatttcg accaccaggc
     1741 ggataccgtg gcgatccgat tcatccctta aatcggcaat cccaataatt tttttatcgc
     1801 gaactaaatc agcaattttt ttgactaatt cagccttatt aacttggtaa ggcagttcag
     1861 taatcacaat cctgtttttg ccggatttag tggtttcaat ctccgctttg ccccggacat
     1921 caaatttacc ccggccggta gtatacattt caataattcc ccggcggccg tgaatttcgc
     1981 cagcagtcgg aaaat
//
LOCUS       DAOOIE010000103         1979 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_45649_length_1979_cov_2.601871, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000103 DAOOIE010000000
VERSION     DAOOIE010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45649_length_1979_cov_2.601871"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="PK131_02815"
     CDS             <1..749
                     /locus_tag="PK131_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45083.1"
                     /translation="GSCDDSTGNYSCQAVNYWDPNDPKLYLTKTQCEQAWGCGDKWRQ
                     GKCDWTTGKWICSRCTNPNDLTGCNYESESSCVEAIGCTVPVTRYSRVEGSCVNSRWQ
                     CQENQNGNFSDLNLCQNGACDEKKEWVYDTCNTQTGNWSCHKTTPDLGVFTTEEDCQK
                     FDGCEVEKKCSVCSRAGYNGPDKSGGDYNCDGVVNLGDYAVWLNEFIKLVYREAITGY
                     EADGDCFRNPELNFGTSLSDYSFWRDKYLH"
     gene            752..>1979
                     /locus_tag="PK131_02820"
     CDS             752..>1979
                     /locus_tag="PK131_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45084.1"
                     /translation="MKQSKFNLLVLLGILAVSIAISIWLTKQNQEVRRKAAGEAYRQY
                     FEPVGVSMKVGETAVIKAMVQSSTEFDGTTLVLCYPKDYLAKITGADIKWGSSFDTSQ
                     SILREINTADSSKLCVQLDAMISYEEETKKPNGTVEVASITLKGLKEGGNLKVEVIKD
                     QLKDAVTGKITQSIIVGTFDPQKPLDTVRYFDSVGEVLVTINGKAVKYSRGACDFTKG
                     LYSCQADENGTMDLANCQKGDAGCYQAYSHGSSSTDCDAVTGNWICKIDGNSRKTLSQ
                     CQIDAGCDINGDNYVLKFKMVYPGVNKNSQCDNGYDQVKVTVESPTGEVWKSDKVKLQ
                     KTTETKDIGENQPVIVYQGEVALTGFNYNEKLSVMIKGPKHLGLVYGKNGQTECYKDR
                     VGTIGGLTKNSATTPVW"
BASE COUNT          612 a          307 c          507 g          553 t
ORIGIN      
        1 aagggagttg tgatgacagt accggtaatt actcttgcca agcggttaat tattgggacc
       61 ctaatgatcc caagctttat ctaactaaaa cgcagtgtga acaggcttgg ggctgcggtg
      121 ataaatggcg ccagggaaaa tgtgattgga ccactggcaa gtggatttgc agtcgatgca
      181 ccaatcctaa cgatttgacc ggctgtaatt atgaatcgga atccagttgt gttgaggcta
      241 ttggttgtac tgttccagtg actaggtatt caagagttga gggcagttgt gtgaatagcc
      301 gctggcaatg tcaagaaaac caaaatggta atttttcaga tttgaatttg tgtcaaaatg
      361 gtgcctgtga tgagaagaaa gagtgggtct acgacacctg taatactcaa acaggtaact
      421 ggagttgtca taaaactacc cctgatttgg gagtatttac taccgaggaa gattgccaga
      481 aatttgacgg ctgtgaggtt gaaaagaagt gttcagtttg cagtagggca ggttataatg
      541 gtccggacaa atccggtggg gattataatt gcgacggagt ggttaatttg ggcgactatg
      601 ccgtttggct gaatgaattt ataaagcttg tttataggga ggccataacc ggttatgaag
      661 cggatggtga ttgcttccgt aaccccgagt tgaattttgg tactagtctg tcggattatt
      721 cattttggcg agataaatat ctccattaaa aatgaaacag tcaaagttta atttattggt
      781 tttgttggga attttagcag tatcaatcgc gatttcaatt tggttgacta aacagaatca
      841 ggaggttaga cgaaaagccg ctggtgaagc ctatcgtcag tattttgagc cggtcggtgt
      901 atcaatgaaa gtaggagaga cagcggtcat taaggcaatg gttcaatcgt cgaccgaatt
      961 tgatggaact actttagttc tttgttatcc taaagattat ctggccaaaa taaccggggc
     1021 agatatcaag tggggaagct cttttgatac ttcccagtcg attctgagag aaataaatac
     1081 agctgattcg agtaaattat gtgttcaatt agatgctatg atcagttacg aggaagaaac
     1141 gaaaaaaccc aatggaacag tggaagtagc ctcaattaca ttaaagggac taaaagaggg
     1201 cggtaatttg aaagtcgaag taataaagga ccaattaaaa gatgcggtta ccggcaagat
     1261 aacccaatca attattgttg gtacttttga tcctcaaaag ccgttagata ccgttagata
     1321 ttttgattcg gtaggtgaag ttttggtgac aatcaacggc aaggcggtga agtacagccg
     1381 cggcgcctgc gattttacta agggtttata tagttgccaa gctgacgaaa atggcactat
     1441 ggatttagcc aattgccaaa agggagatgc aggttgctat caggcctatt cccacggtag
     1501 cagttctaca gattgtgatg ccgtaaccgg caattggata tgtaaaattg atggcaacag
     1561 tcgtaagact ttgagtcagt gccaaattga tgccggttgt gatattaatg gtgataatta
     1621 tgttctaaaa tttaaaatgg tttatccggg ggtaaacaaa aatagtcaat gtgataatgg
     1681 ttatgatcag gtaaaagtga cagtggagtc accgactggg gaagtctgga aatcagataa
     1741 agttaaactc caaaaaacaa cagagactaa agatatcggt gaaaatcaac cggtaatagt
     1801 ttaccagggt gaagtcgctc taactggttt taattataac gaaaagttgt cagttatgat
     1861 taagggacct aaacatcttg gtttggttta tgggaagaac ggacaaaccg agtgctataa
     1921 ggatagggta ggaactattg gtggtttaac taagaacagt gctactacac cagtttggg
//
LOCUS       DAOOIE010000104         1972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_45882_length_1972_cov_4.145540, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000104 DAOOIE010000000
VERSION     DAOOIE010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45882_length_1972_cov_4.145540"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="PK131_02825"
     CDS             <1..517
                     /locus_tag="PK131_02825"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPD45085.1"
                     /translation="GPVFFKQKRAGKNHQEFLMYKFRTMYTGNNDERLRKNYPHLWKK
                     YKANGWKLPMAEDPRITPVGRILRFFSIDELPQLINILKGEMSLVGPRAYREEELEEY
                     EKKFPHTQKYIEDIRSVRPGLSGVWQTSGRNELPFEKRAKLDSDYVRHRSFRQEILIL
                     LKTPFSMISRW"
     gene            547..>1972
                     /locus_tag="PK131_02830"
     CDS             547..>1972
                     /locus_tag="PK131_02830"
                     /inference="COORDINATES: protein motif:HMM:NF024594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HPD45086.1"
                     /translation="MRLKKFLISFLSILAIIIACLTIFFFFFAFVPGKNLYYQLLTTK
                     NKFTEIQQSFTAKNFDQIKNQLDDLNNQKQIIQKKYIGLKIVGSLPHFNRYYRDGQHA
                     LNIFQEGLETGKIIVQAIEPYKDFLGLQGVATDSAQTTQDRITFLTESIEGIVPHLST
                     IENKLSQIEKELDQIDPNRYPEEFHGLKIKSNLLKAKEGLSEAIKLLKNGQPILSQAS
                     WFLGKDSPRNYLLMFQNDAELRPTGGFWTAYALIKVDKGKFTPILSEDIYALDTRYNS
                     RVPAPRPIKAYHINVPYFNLRDLNISPDFSVSMGNFMEIYNKISPNKTKIDAVVAIDT
                     HILVDLVKILGRVGVSGFGNFSADPDKRCDGCPQIIYQLEWLAGRPRNYLETNRKGFL
                     GPLMNSILSNIMGSEKSKIGPLAEAFLNNIYQKHILFYFPDETMENAAKSINIAGAIT
                     PTDGDYFHLNDANMASAKTNLFLKK"
BASE COUNT          640 a          391 c          332 g          609 t
ORIGIN      
        1 ggggccagtt ttttttaaac aaaaaagggc aggcaaaaac catcaggaat ttttgatgta
       61 taaattccga acaatgtata ccggtaataa tgacgaacgt cttagaaaaa attacccaca
      121 tttatggaaa aaatacaagg ctaacggttg gaagctaccc atggctgagg atccccgaat
      181 tactcctgtc ggtcgaattc tccgcttctt ttccattgac gaattacccc aattaattaa
      241 tattcttaaa ggggaaatga gtttagttgg cccacgagcc taccgtgagg aagaattaga
      301 ggaatatgaa aaaaaatttc ctcatactca aaaatacatt gaagatattc gctcggttcg
      361 accaggtctc agcggcgttt ggcaaacttc cggccgtaat gaacttcctt ttgaaaaacg
      421 ggctaaactt gactcagatt atgttcgtca ccgttccttt cgtcaagaaa ttttaattct
      481 tttaaaaaca cccttttcga tgatctcacg ctggtaaaat tgagttaaac cctttacaat
      541 taatccatgc gtctgaaaaa gtttttaatt tccttcctgt ctattttagc aattattatt
      601 gcctgtctaa ctatcttttt tttcttcttc gcttttgttc ccggaaaaaa tctttactac
      661 caattattaa caactaaaaa taaatttaca gaaattcaac aatcatttac cgctaaaaat
      721 tttgaccaaa ttaaaaacca attagatgat ttaaacaatc agaagcaaat tattcaaaaa
      781 aaatatatcg gtttaaaaat tgtcggctct ttgcctcact tcaaccgtta ctatcgtgac
      841 ggtcaacatg ctctcaacat ttttcaggag ggactagaaa caggaaaaat catcgttcaa
      901 gctattgagc cctataagga ttttctcggt ttgcaaggag tagccactga ttctgctcaa
      961 actactcaag atcggatcac tttcttaacc gaaagtattg aaggaattgt ccctcaccta
     1021 tcaacaattg aaaataaact gtctcaaatt gaaaaggaat tggaccaaat cgatcccaat
     1081 cgttatccag aagaatttca tggcttgaaa atcaaatcaa atttactaaa agctaaagaa
     1141 ggtttaagtg aagctattaa actccttaaa aacggccaac cgattttatc gcaagcttcc
     1201 tggtttctag gtaaagattc cccaagaaat tatctgctaa tgtttcaaaa tgatgccgaa
     1261 ttacgtccta ctggcggttt ttggactgcc tatgctctta ttaaggttga caaaggaaaa
     1321 tttaccccta ttttgtccga agacatctat gctttagata cccggtataa cagccgagtt
     1381 ccagccccac gacctattaa ggcctatcac atcaatgttc catattttaa tcttcgggat
     1441 ctaaatatct ctccagattt ttctgtaagt atgggtaatt ttatggaaat ctataataaa
     1501 atctccccta ataaaaccaa aattgatgcg gtagtggcca ttgataccca tattctagtt
     1561 gatttggtca aaattttggg tagagtagga gtttcaggtt ttggtaattt ttcggctgat
     1621 cctgacaaac ggtgtgatgg ctgtccgcaa attatttatc aactggaatg gctggctggc
     1681 cgaccccgta attacctgga aactaatcgt aaaggctttc tgggaccctt aatgaactcg
     1741 attctaagta atattatggg atcggaaaaa agtaaaattg gacctttagc cgaagctttt
     1801 ttaaataata tttatcaaaa acacattctc ttttattttc cagacgaaac tatggagaat
     1861 gcggctaaat caatcaacat tgctggggca attactccca ctgacggtga ttattttcat
     1921 ctcaatgatg ctaatatggc cagcgctaaa accaacttat ttctcaagaa aa
//
LOCUS       DAOOIE010000105         1888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_48907_length_1888_cov_3.280415, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000105 DAOOIE010000000
VERSION     DAOOIE010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48907_length_1888_cov_3.280415"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="PK131_02835"
     CDS             complement(<1..722)
                     /locus_tag="PK131_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013012125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HPD45087.1"
                     /translation="MSNKIIGIDLGTTNSCVAVVEAGTPKVILTSEGRNVIPSVVEPV
                     KKLVGDLAKRQMVLNPQNTVYSIKRLMGRRIDDPEVKKTEKVAPYKIVAGKDKMAAVE
                     INGKVYTPQEISAMILQKAKTDAEKYLGEKVESAVITVPAYFDDSQRQATKQAGEIAG
                     FKVERIINEPTAASLAYGLDKKSSGLVAVYDLGGGTFDISILEIGGGVFEVKATNGDT
                     FLGGDDFDQKIITWIVDDFQKQ"
     gene            complement(736..1176)
                     /gene="grpE"
                     /locus_tag="PK131_02840"
     CDS             complement(736..1176)
                     /gene="grpE"
                     /locus_tag="PK131_02840"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPD45088.1"
                     /translation="MTKTAKIADLEEKLARSLADYANLERRVANQKQFFTSLASAAVM
                     VKIIDALDELHLAADHLQDPGLKMALDKLMKALQSEGLEEIAAVGQIFDPKIMDCVDV
                     AEGKQDEVVSVRKKGYLLNGECLRPAQVVVGKEIVDRRKLKIKN"
     gene            complement(1184..>1888)
                     /locus_tag="PK131_02845"
     CDS             complement(1184..>1888)
                     /locus_tag="PK131_02845"
                     /inference="COORDINATES: protein motif:HMM:NF013768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45089.1"
                     /translation="KCVTEEFIETAASVGSETLEKKYSLGVSSATIRNEMAVLTKLGY
                     LKKNHLSAGHTPTSLGLKYYVRNLMSPKGLSVVEELGVKEKVWDYRNEFEKLLREITR
                     ELANRTRSLALSTIDQGVIYSYGASNLLEEPEFFNIDVAKTALSLVDNTKFWFDIISK
                     SRAVPEAEEKQTVHLLIGEDLGLEHLEPCGFIYQDYQAGPHRGILGVLGPARFRYPNI
                     IPIIDYFAELVSTITH"
BASE COUNT          562 a          444 c          317 g          565 t
ORIGIN      
        1 ttctgcttct gaaaatcatc aacaatccaa gtaataatct tctgatcaaa atcatcacca
       61 ccaaggaaag tatcaccatt ggtagcttta acttcaaaga caccgccacc aatttccaaa
      121 attgaaatat caaaggtgcc gccgcccaaa tcataaaccg ccactaatcc ggaacttttc
      181 ttgtccaaac cataagctaa agaggcagcc gttggctcat taataattct ttctacctta
      241 aaaccggcaa tttctcctgc ctgtttagtt gcctgacgtt gggaatcatc aaaataagcc
      301 ggcaccgtaa ttactgccga ttctactttt tctcccaaat atttctcagc gtcggttttg
      361 gctttctgca aaatcatggc tgaaatttcc tgaggggtat aaaccttgcc attaatctcc
      421 actgccgcca ttttatcctt accggcaaca attttataag gagctacttt ttcggttttt
      481 ttcacttcgg gatcatcaat tcgtcgaccc atcaaacgct taattgaata aaccgtattt
      541 tgcgggttta aaaccatctg acgtttagcc agatcgccaa ctaatttctt taccggttca
      601 actactgaag gaataacatt gcggccttct gaagttaaaa taactttagg agtaccagct
      661 tcaactacgg ctacacacga attcgtggtg cctaaatcaa taccaattat tttatttgac
      721 atacttattt aatttttaat ttttaatttt taattttcta cgatctacga tctcttttcc
      781 tacaactacc tgggcagggc gaagacattc gccatttaaa agataaccct ttttacgaac
      841 agaaacaacc tcgtcctgct tcccctcggc aacatcaaca caatccatta tcttggggtc
      901 gaaaatctgg ccgacagcgg caatttcctc caaaccctcc gattgtaaag ctttcattaa
      961 cttatctaaa gccattttaa ggccggggtc ctgaaggtga tcggctgcca gatgaagttc
     1021 gtcgagggca tcaataattt tgaccataac ggcggctgaa gccaaagagg taaaaaactg
     1081 tttttgatta gcaacccgcc tttctagatt agcataatct gctaaagatc gggctaattt
     1141 ttcttccaaa tcggctattt tagctgtttt agtcataagc aatttaatgg gtaatagtag
     1201 aaaccaattc ggcaaaataa tcaattattg gaataatatt aggatagcgg aaacgagccg
     1261 gacctaaaac ccccaaaatt ccccgatggg gaccagcctg ataatcctga taaatgaaac
     1321 cacagggctc caggtgttcc aaacccaaat cttcaccaat taagagatga actgtctgtt
     1381 tttcttccgc ttccggcact gcccggcttt tgctgataat atcgaaccag aatttagtat
     1441 tatccactaa agatagggcg gttttagcga catcaatatt aaaaaactcc ggttcttcaa
     1501 gtaaatttga agcaccatag gaataaatta ctccctgatc aatagttgat aaggctaagg
     1561 aacgggttcg attggccagc tctcgagtta tttcccgcaa taatttttca aattcattcc
     1621 gataatccca cactttctct ttcactccca attcttcaac taccgataag cctttgggcg
     1681 acatcaaatt acggacataa tatttcaatc ccaatgaagt cggagtatga ccagccgata
     1741 aatggttctt ttttaaatag cctagcttgg ttaggacggc catttcattc ctgatagttg
     1801 ccgaagaaac tcctaaagaa tattttttct ctaaagtttc tgatccgacc gaagcggccg
     1861 tttcgataaa ttcctcggta acacactt
//
LOCUS       DAOOIE010000106         1881 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_49175_length_1881_cov_2.878423, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000106 DAOOIE010000000
VERSION     DAOOIE010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49175_length_1881_cov_2.878423"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="PK131_02850"
     CDS             complement(<1..211)
                     /locus_tag="PK131_02850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003491515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD45090.1"
                     /translation="MANKPLISAVINVRNEADNLDKCLKSIKNLVDEIVVVDMHSTDN
                     SVAIAQKYGAKIYPHRFLRVVEPARN"
     gene            complement(227..1816)
                     /locus_tag="PK131_02855"
     CDS             complement(227..1816)
                     /locus_tag="PK131_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45091.1"
                     /translation="MLSRQKITFWSCLLICTFFQVLPVIRSGWPSTAGLSFWGPTGHD
                     GIWHLSLINHLHNPFKIDLPTMAGESLTNYHPFFDILAKFLASLTHLPSTLILFQILP
                     ILMAVSFLYLSFLIGGYPLMLLNTFATSLGWLFGKGETAFWAMQSPSNQLNPPFMLSL
                     VGLSLLIYLIYRKNKLNGFHLALIFLILTFLPITKIYAAPTGFFLFLIFSLKKIKSDR
                     RPLWILLSSLITSVVLFFIYNPNLSATLIYKPLWFVNTMFESQDRFFLPQITNIIYTL
                     KASPYFSLRLLIVEIFGVCLFIIGNYAFRILGFLKTNKEFLFLILFNTLVPLLFIQKG
                     TAWNTIQFLYYGLFIGNIALASYLRNKKTFLAVIIIISFIANFPIYKNYLSNPAPTAI
                     FPDEIKALNFLHSQTPGIVLTHPYDPYLKNKFSSTPLPIYAYETTAYVSAFSQQLVFL
                     EDEMNLQILDKNYLQRRQESLTFFDQKNEFQNRGFLVNNQIDYIYLPKDFPDRISQNY
                     LALGIKKIFENNSVLLFSVER"
BASE COUNT          692 a          267 c          398 g          524 t
ORIGIN      
        1 aattgcgggc tggttcaact accctaagaa aacggtgagg gtatatttta gccccgtatt
       61 tctgagctat ggcaacacta ttatcagttg agtgcatatc aaccaccaca atttcatcaa
      121 ccaagttttt aatagatttt agacatttat ctagattatc cgcttcgttt ctgacgttaa
      181 ttactgccga aattaagggt ttatttgcca tgatattttt attttatcac ctttcgaccg
      241 aaaagagtaa aacagaatta ttttcaaaga tttttttaat acccaatgct agatagtttt
      301 ggcttattct atcgggaaaa tctttgggaa ggtaaatata atcaatttga ttattaacca
      361 aaaagccacg gttttgaaat tcattttttt gatcgaaaaa agtcagggat tcttggcggc
      421 gctggagata gtttttatct aaaatttgga ggttcatttc atcttctaag aaaactaatt
      481 gttgtgaaaa tgctgataca taggcagttg tttcataagc ataaatgggc aggggggtag
      541 aagaaaattt gtttttaagg taaggatcgt aaggatgagt taatactatt cctggagttt
      601 gagaatgaag aaaattcaga gccttaattt catctggaaa aatggctgtt ggagccggat
      661 tactcaaata gtttttatag atagggaaat tagcaatgaa ggaaattatg ataattacgg
      721 ccaaaaaagt ttttttgttc ctaagatagc tggctaaagc aatattacct ataaataagc
      781 cataatatag gaactgaata gtattccaag ccgttccttt ctgaataaat aaaagaggaa
      841 ctagggtatt aaataagatc aaaaagagaa attctttatt agtttttaaa aaccccaaaa
      901 ttctaaaggc ataattgccg ataataaaga gacaaacacc aaaaatttcg actattagca
      961 gccgcaggct aaaataaggc gaggccttta gggtgtaaat tatattggta atttggggaa
     1021 gaaaaaatcg gtcttgggat tcaaacatag tgttgacgaa ccaaaggggc ttatagataa
     1081 gagtagcgga aagattggga ttataaataa agaataaaac gacgctggta atcaaagagg
     1141 aaagtaagat ccataatggc cgccggtcgg atttaatttt tttaaggctg aagataagaa
     1201 atagaaaaaa tccggttgga gcggcataaa ttttagtaat tggcaaaaag gttaaaataa
     1261 gaaagattaa ggccaaatgg aaaccattta gtttattttt acggtaaata aggtagatta
     1321 gaagggaaag accgaccaga gagagcataa acggagggtt taactgatta gagggtgatt
     1381 gcatggccca gaaggcagtt tcacctttac caaaaagcca gcccaaagaa gtagcgaaag
     1441 tattcaaaag cattaaaggg taaccgccga ttaaaaaact gaggtacaga aaggagacag
     1501 ccataagaat aggaagtatt tggaagagaa ttaaagtcga aggtaaatga gttaacgagg
     1561 ctaggaattt agctaaaata tcaaaaaaag gatggtagtt ggtcagactc tcccctgcca
     1621 tagtgggtaa gtcaatttta aaaggattat gaagatgatt aattaaactg aggtgccaga
     1681 tgccatcatg gccggttggc ccccaaaaac tcagaccagc agttgatggc caaccgctgc
     1741 gaattacagg caaaacctga aaaaaagtac atattaacaa acaagaccag aaagttattt
     1801 tttgacgaga tagcatttga gttgtttaat aaaccagtta atttcttgac ggcagaaaaa
     1861 ccattgataa aggcaataac t
//
LOCUS       DAOOIE010000107         1876 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_49375_length_1876_cov_2.372872, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000107 DAOOIE010000000
VERSION     DAOOIE010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49375_length_1876_cov_2.372872"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1578
                     /locus_tag="PK131_02860"
     CDS             <1..1578
                     /locus_tag="PK131_02860"
                     /inference="COORDINATES: protein motif:HMM:NF014895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HPD45092.1"
                     /translation="KTNWSLIQKTHIPQEIKLIKSQQETKEVKIPKKDQNKPKLTDEL
                     VLELARISQKLHNYYTKPQDIEFAIEKGKIYIVQTRPITTVINLQKILDNQSRIDQTA
                     DLTGEPASPGLATGKIIIINSPKEINRVGQNQILVTTMTTPDFVPAMKKVNGIITDKG
                     GQTSHAAIVSRELGVPCVVGAKIATKFFKEGETVTIDGTTGKIWNGNLIANNTFHLPG
                     KPINFQPNLKTATKIYVNLGEPDLAKEMAQKNCDGIGLLRAEFMIANIGIHPKRIIAE
                     HKEKNYIDKLAKDMVKFCQAFSPRPVIYRATDFKTNEYRFLKYGSLYEPIEPNPLMGF
                     RGASRYITSPDVFMMELEAIKQVRNKYGYKNLWLMIPFVRTPDELFKVKKIIAAAGLL
                     RSPSFKLFMMAEIPSNVIILDKFIDVGIDGISIGSNDLTMLTLGLDRDNAEVANNFNE
                     MDPAVLWSLRHLIKKAHKRKILVGICGQAPSNYPDLVEKLVKWGIDSISVSPDAIDRT
                     REIVAWAEKRKIRQKHP"
     gene            complement(1559..>1876)
                     /locus_tag="PK131_02865"
     CDS             complement(1559..>1876)
                     /locus_tag="PK131_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA (cytidine-2'-O-)-methyltransferase"
                     /protein_id="HPD45093.1"
                     /translation="SGLPPSRFSFLGFLPKRSADRMKILSNYKEIEGVKIVYESPYRI
                     QRLIGEIKAIYGDNAEIIVCREMTKKFEEIIHVGTCQGRNPGAFPENAKGEITVVFKG
                     VSV"
BASE COUNT          616 a          375 c          329 g          556 t
ORIGIN      
        1 aaaaccaatt ggagcttgat tcaaaaaacc catattccac aagaaattaa attaattaaa
       61 tctcaacagg aaacaaaaga ggttaaaatt cccaaaaagg atcagaataa acctaaatta
      121 actgatgaat tagttttaga actcgctcga attagtcaaa agcttcacaa ttattacacc
      181 aaacctcagg atattgaatt tgccattgaa aagggtaaaa tttacattgt tcaaaccaga
      241 ccgatcacca cggttattaa tcttcaaaaa attctcgata atcaatctag aattgatcaa
      301 actgccgatt taaccggtga accagcttct cctggtttgg ctactggtaa aatcatcatc
      361 atcaattctc caaaggaaat taatcgggtt ggtcaaaacc aaatcttagt tactactatg
      421 accactcctg attttgtccc agcgatgaaa aaagttaatg gtattattac cgacaagggt
      481 ggtcaaactt ctcacgccgc tattgtctct agagaattgg gtgttccttg tgtcgtcgga
      541 gctaaaatcg ctactaagtt ttttaaagaa ggggagacag taactattga cggtactact
      601 ggtaaaattt ggaatggtaa tttaatagcc aataatactt ttcatctacc cggaaaacca
      661 attaatttcc agcctaactt aaaaacagcc actaaaatct atgttaatct cggagaaccg
      721 gacctagcta aagaaatggc tcaaaaaaat tgtgatggta ttggtcttct ccgagccgaa
      781 tttatgatcg ccaatatcgg tattcatccc aaaagaatta ttgccgaaca taaggaaaaa
      841 aattacatcg ataaattagc caaggatatg gttaaatttt gtcaagcttt ttcccctcgg
      901 ccggtaattt atcgagctac cgactttaaa accaacgaat atcgctttct aaaatatgga
      961 agtttgtacg aaccaatcga acccaatcca ttaatgggtt ttcgtggagc cagccgttat
     1021 attacctcac ccgatgtttt catgatggag ttggaggcca ttaaacaagt taggaataaa
     1081 tatggctata aaaatctctg gttaatgatt ccttttgtcc gtactcccga cgaattattt
     1141 aaagtcaaaa aaattattgc tgccgccgga ttgcttcgct ccccttcatt taaacttttc
     1201 atgatggctg aaattcccag taatgtaatt attttggaca aatttattga tgtcggtatt
     1261 gacggcattt ccattggtag taatgatcta accatgttaa ccttaggact tgaccgagat
     1321 aacgccgaag ttgccaataa ttttaatgaa atggatccgg cagttctctg gagcttgcgt
     1381 catttaatta aaaaggcgca taagcgaaaa attttagtcg gtatttgcgg tcaagcacca
     1441 agcaattacc cagatttagt agaaaaactt gtcaaatggg gaatagatag tatttctgtt
     1501 tcacctgacg ctattgaccg aactcgggaa attgtcgctt gggcagaaaa acgaaaaatc
     1561 agacagaaac acccttaaat accactgtta tttcgccttt ggcgttctca gggaacgcac
     1621 ccggattgcg tccttgacac gttcctacat gaataatctc ctcgaatttt ttggtcattt
     1681 ctcgacagac aataatctcc gcattatctc cgtaaatagc tttaatttcg ccaattaacc
     1741 tctgaatccg atagggtgat tcataaacga ttttcacccc ttcaatttcc ttgtaattac
     1801 ttaaaatttt cattctatcg gctgatcgtt taggtaaaaa tccgagaaaa ctaaatcggc
     1861 tcggaggtaa accgga
//
LOCUS       DAOOIE010000108         1848 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_50489_length_1848_cov_3.238148, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000108 DAOOIE010000000
VERSION     DAOOIE010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50489_length_1848_cov_3.238148"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PK131_02870"
     CDS             complement(<1..651)
                     /locus_tag="PK131_02870"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HPD45094.1"
                     /translation="MKTNPNLEKLEDKSFLLKATISQDKIKAARQQALVKIQSTFKTE
                     GFRPGKAPIEVVEQKVNPQTLLEKVIQTVLTENYADEIKKNDLHPIIDPEVKITTSPL
                     DYDHNWEVEYRSCELPEVKIDAVAFEEIKKLDGERKIDQIFDLLIKHAVILLPERLKE
                     QEKDWKINLIIDKIAADQKLEVTTEEIRKTLEVNPQLAQNINLTFYLMRQQKVLDYL"
     gene            683..1297
                     /locus_tag="PK131_02875"
     CDS             683..1297
                     /locus_tag="PK131_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45095.1"
                     /translation="METKLNFISHRIFKGVLGLVVIVMIGYGFKLTRFNSIDKKHFNM
                     SDLSGRVQIAMAEQPAIASDKKIYDQVIGVDARPLIIEKYLEKYHSPLLPYADLIFNL
                     SQTYGFEYYWIVAIAQQESNLCKKAPENSFNCWGYGIHQAGTLKFDNYEIALKSFAEY
                     LKREYFDKGYNTAELIMEKYCPNSSGSWAYGVNQFIEEMESGNF"
     gene            1396..>1848
                     /locus_tag="PK131_02880"
     CDS             1396..>1848
                     /locus_tag="PK131_02880"
                     /inference="COORDINATES: protein motif:HMM:NF019814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPD45096.1"
                     /translation="MTLVSTAQFRKGMYLLFQDKPHMIVDITFVSPGRGCAFYRVKFK
                     NLFTGRLVEYTYKSGEKVEEVLVETREAEYSYFDGLNYVFIEPRTFEQYSITAEVIGD
                     DKVYLKPGALYRIKFYEDQSVGVVLPKSISSKVIKTENAIKGNTATNAL"
BASE COUNT          541 a          289 c          385 g          633 t
ORIGIN      
        1 aaggtagtca agtacttttt gctgtcgcat aaggtaaaaa gttaggttga tattttgagc
       61 cagctgaggg ttaacctcaa gagtttttct gatttcttca gtagtaactt ctaatttctg
      121 atcggcagca attttgtcga taataaggtt aattttccaa tccttttcct gttctttaag
      181 cctttccggc agaagaataa cggcgtgttt aatgagaaga tcgaatattt ggtcgatttt
      241 tctttctccg tccagttttt tgatttcttc aaaagcaacg gcatcgattt taacctcggg
      301 cagttcacag gaacgatatt caacttccca attatggtca tagtcaagag gagaggtggt
      361 aatttttact tccggatcaa taataggatg aagatcattt tttttgattt catcggcata
      421 attttcagtt aaaacagttt gaattacttt ttccaataaa gtttgaggat tgactttttg
      481 ttcaaccact tcaattgggg ccttacccgg ccggaagcct tcggttttaa aggtcgattg
      541 aattttaact aaagcctgct ggcgggcggc tttaatttta tcttgggaaa tagtagcttt
      601 aagaagaaaa gatttgtcct caagtttttc taaatttggg tttgttttca tcagtaggct
      661 tatgataaca tacatttact aaatggaaac gaaactaaat tttatcagcc atcggatttt
      721 taaaggagtt ttgggattgg tagtgattgt tatgattgga tacggattta aattgacccg
      781 ttttaattca atagataaaa aacatttcaa tatgagcgat ttatcgggta gggttcagat
      841 agcgatggct gaacagccgg ctatagcttc cgataagaaa atttatgatc aggtaattgg
      901 ggtggacgcc aggccgctga ttattgaaaa atatttagaa aaatatcatt ctccccttct
      961 cccttatgcc gatttgatct ttaatttatc ccaaacttac ggctttgagt attattggat
     1021 tgtggccatt gcccaacaag agtcaaattt gtgtaagaag gcgccggaga atagttttaa
     1081 ttgctggggt tatggtattc atcaggccgg aactttaaag tttgataatt atgagatagc
     1141 tcttaagagt tttgctgaat accttaaaag ggaatatttt gataaggggt ataatacagc
     1201 tgaactaata atggagaagt attgccctaa ttctagcggt tcttgggcct atggtgtcaa
     1261 tcagtttatt gaagaaatgg agtccggaaa cttctagttg actttcttat aatattttga
     1321 tatactcaaa ataggtcctg gggtggctcc tctaaaaagc agactctggg atttttttat
     1381 gctaaaattc ctcttatgac tttagtcagt accgcccagt ttagaaaagg aatgtatctc
     1441 ctctttcagg ataagcccca tatgattgtg gatattacct ttgtttctcc aggaagagga
     1501 tgtgcttttt atcgagtcaa attcaagaat ttatttaccg gacgattagt agaatatacc
     1561 tacaaatcgg gagaaaaagt cgaagaagtt ttggtggaaa ctcgagaggc cgaatattct
     1621 tattttgatg gtttaaatta tgtttttatt gaacctcgga cctttgagca atattctatt
     1681 acggccgaag taattggaga tgacaaggtt tatcttaaac cgggagcatt gtatcggatt
     1741 aaattttacg aggatcaatc agtaggcgtt gttttaccca aatcaatttc aagtaaggtc
     1801 attaaaactg aaaatgccat taagggtaat acggcaacta atgcttta
//
LOCUS       DAOOIE010000109         1833 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_51151_length_1833_cov_2.419573, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000109 DAOOIE010000000
VERSION     DAOOIE010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51151_length_1833_cov_2.419573"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="PK131_02885"
     CDS             <1..731
                     /locus_tag="PK131_02885"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HPD45097.1"
                     /translation="KSGFSRGFYNDLKNIGIVHIVVVSGMNVMILARFFIESLAGLIG
                     RKWAILLGLSVIWFYSLMIGWQPPILRAVLLVTFLYWAQLLGRKFNLGRVIVLIILMM
                     ILADYTMLSQVSFWLTFIGFGAVVLDPYWVWLWITPVLALVFGRISLIAPLANALVLF
                     LVEIITIIGGLGAVLNSKFILWLIMPLLRYIIIVTNLLGQLKWSSLTIKFNWPMMIGS
                     YLLLIWFYLKRRDLKQKKPNFTGS"
     gene            691..1404
                     /locus_tag="PK131_02890"
     CDS             691..1404
                     /locus_tag="PK131_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45098.1"
                     /translation="MILSRKNQILLAVSGLLLAFWFWQVRPDNRTRVIFCNVGQGDGA
                     IVIKNNFQMLIDAGPQNSKMAECLGRYVPFWDKEIEVFVASHSDQDHIGGLAAIKRGY
                     HLEKEITYQNIDAGDVIRYDGIDFVVLSNSELSLIGVLKTGSKKVLFMGDADVGMEYQ
                     IKEPVNILKVGHHGSETSTSEVFLDRIKPQIAVISVGRGNIYGHPKQAILDRLSARGI
                     EIKRTDEEGDLIFDLSLDK"
     gene            complement(1376..>1833)
                     /locus_tag="PK131_02895"
     CDS             complement(1376..>1833)
                     /locus_tag="PK131_02895"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPD45099.1"
                     /translation="DNNAYRLLLDHIDNDLVYKVYTDLGVDISKAKNNPTGNVLSVSG
                     YASFFRILYNASYLNRSMSEKALNLLSQTTFHQGLVAGVPNNITVAHKFGERHYTNTG
                     EYQLHDCGIIYVPDKPYLLCVMTRGNNFNNLSQIISQISETIYQEINQK"
BASE COUNT          545 a          249 c          401 g          638 t
ORIGIN      
        1 acaaaagtgg cttttctagg ggtttttata acgacttaaa aaatattggt attgttcata
       61 ttgtggtagt tagtggtatg aatgtaatga ttttagcccg ctttttcatt gaaagcttgg
      121 cagggttgat aggtagaaag tgggcaattt tgttaggatt atcagtaatt tggttttata
      181 gtctaatgat tggttggcaa ccgccaattt tgagggcagt tttattggta acttttcttt
      241 actgggctca actcttggga agaaaattta atcttggtcg agtaatagtt ttaataattt
      301 taatgatgat tttggctgat tatactatgc tatcccaggt tagtttttgg ttaactttta
      361 ttggatttgg agcagtagtt ttagatcctt attgggtatg gctttggatt actcctgttt
      421 tagctctggt gtttggtagg ataagtttga ttgctccttt agctaatgct ttagttttgt
      481 ttttagtgga aataattact attattggcg ggttaggggc tgttttaaac agcaaattta
      541 ttttatggct aattatgcct ttactgcggt atatcattat tgttaccaat cttttaggtc
      601 agttaaaatg gtctagcctg acaattaagt ttaattggcc aatgatgatc ggcagttatt
      661 tattattgat ttggttttat cttaaaaggc gtgatcttaa gcagaaaaaa ccaaatttta
      721 ctggcagtta gcggtctact tttggccttt tggttttggc aagtaaggcc agataatcgg
      781 acccgagtaa ttttttgtaa tgttggacaa ggagatggtg caattgttat taaaaacaat
      841 tttcaaatgc ttattgatgc tggtcctcaa aatagcaaaa tggctgaatg tttaggtagg
      901 tatgttccat tttgggacaa agaaatagaa gtttttgtgg cttcacacag cgaccaagac
      961 catattggtg gtttagcggc gataaaaagg ggataccacc ttgaaaagga gattacctac
     1021 cagaatattg atgccggtga cgtaattaga tatgatggaa ttgattttgt tgtccttagt
     1081 aacagcgaat tatctttaat tggagtattg aaaactggga gtaagaaagt tttgtttatg
     1141 ggagatgctg atgtaggcat ggaatatcaa attaaagagc cggtgaatat tttaaaagtg
     1201 ggtcatcatg gctcagaaac atcgactagc gaagtctttc tagatagaat taaaccccaa
     1261 atagcagtga ttagtgttgg taggggtaat atttatgggc atcccaaaca ggctattctg
     1321 gatcgtttat cggccagagg catagagatc aaacggactg atgaagaagg cgaccttatt
     1381 tttgatttat ctcttgataa atagtttcag aaatttgact aatgatctga ctaagattgt
     1441 taaaattatt tccccgagtc ataacacaga gaagataagg tttatcagga acgtaaataa
     1501 tgccgcaatc gtgtaattgg tattcgccgg tattggtgta atgtctttcc ccgaacttat
     1561 gagcgactgt tatattattt ggtacaccag ctaccagtcc ttggtgaaaa gtggtttgag
     1621 agagtaaatt taaagccttt tctgacatag agcggtttaa ataagaagca ttataaagaa
     1681 tacgaaaaaa tgaagcatag ccactaacac ttaaaacatt acccgtcgga ttattttttg
     1741 ctttggaaat atcaactccc aaatcagtat atactttata cactagatca ttatcaatat
     1801 gatccaataa aagccgataa gcgttgttat ctg
//
LOCUS       DAOOIE010000110         1819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_51716_length_1819_cov_2.536848, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000110 DAOOIE010000000
VERSION     DAOOIE010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51716_length_1819_cov_2.536848"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PK131_02900"
     CDS             <1..252
                     /locus_tag="PK131_02900"
                     /inference="COORDINATES: protein motif:HMM:NF016370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HPD45100.1"
                     /translation="IFCKKGNIDLECDGERYHTLPEALARDRKRNNQLTSFGWRVLRF
                     SGKEISQDIGNCLRVVERTIGTLGGLGKIKIETLESHCF"
     gene            complement(232..1233)
                     /gene="trpS"
                     /locus_tag="PK131_02905"
     CDS             complement(232..1233)
                     /gene="trpS"
                     /locus_tag="PK131_02905"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPD45101.1"
                     /translation="MLPGTKLRILTGDRPTGKLHLGHWVGSLQNRVKYQDQYQVFLVL
                     ADLHLLTTFPTKEKIKQIKNNIHDIVLDYLAAGIDPEKVVIYQQSQIAAVTYLSTIFQ
                     MLVTVPRAQRIPTLKQVMHDLDLKKPSVGLLIYPILQAADILMVKADLVPVGKDQQSH
                     VELTVELATTFNKLYGNVFPIPKAIIGKTGTLVGLDGRAKMSKSLNNCIYLSDDPATV
                     KEKVNSMYTDPQRIHSSDPGRVEGNPVFIYHDLFNENKEEVVDLKKRYRQGQVSDIEV
                     KEKLALALEKFLAPMREKRREFENKPGLVEEILARGQTVVRPIAQETLNEVLKAMGF"
     gene            1252..>1819
                     /locus_tag="PK131_02910"
     CDS             1252..>1819
                     /locus_tag="PK131_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HPD45102.1"
                     /translation="MAQITHQIKAEIRTAKGRSQVKHLKEAGLIPATLYGKGIASISI
                     QFPAKELIKTFKEAGESVLVELLLDNQKIPVLFKNPQYHYLNGHLIHLDCFRVNLKEK
                     ITAEVPIVLVGESPAVKEGNILVTVSDQINVEALPADLPEKIEVDIGSLTTIGAIITA
                     GDLILDKSKVELKTAADQVIVKIEEPKIE"
BASE COUNT          595 a          358 c          348 g          518 t
ORIGIN      
        1 atattttgta aaaaaggaaa tattgaccta gagtgtgacg gcgaacgata ccacacacta
       61 cccgaagcct tagcaagaga cagaaaaaga aataatcagc ttaccagctt tggctggaga
      121 gtattaaggt tctcaggaaa agaaatcagt caagacatcg gtaattgcct aagagtagta
      181 gaaaggacca ttggcactct aggtggtttg ggtaaaataa aaattgaaac gttagaatcc
      241 cattgctttt aaaacttcat ttaaagtttc ttgggcgatg gggcgaacca cggtttggcc
      301 ccgggcgaga atttcctcaa cgaggccggg tttattttca aattcccgac gtttttcccg
      361 catcggcgct aaaaatttct ctaaagctag agctagtttt tctttaacct caatatcgct
      421 aacctgccct tgacgataac gctttttcag atcaactact tcttctttat tttcattaaa
      481 taaatcatgg tagataaaaa caggattgcc ttcaactctt ccaggatcgc ttgaatggat
      541 tcgttgagga tcagtataca ttgaattcac tttttcttta accgtagccg gatcatcgct
      601 tagataaata caattattta atgatttact cattttggcc cggccatcaa gacccaccaa
      661 agtgccagtt ttaccaataa tagccttagg aatgggaaaa acattaccat aaagtttatt
      721 aaaggtagtg gctagctcta ctgttaattc cacatggctt tgctgatctt taccaacagg
      781 gaccaaatcg gctttaacca ttaaaatatc ggctgcctga agaatcggat agatcagcaa
      841 acctaccgaa ggttttttaa gatcaagatc atgcatcacc tgttttaagg tagggattct
      901 ttgagctcgg ggaacagtta ctaacatttg aaaaatagta gacaaatagg taactgccgc
      961 tatctgtgac tgctgataaa taactacttt ctctggatca attcctgctg ctaaataatc
     1021 caagacgata tcatgaatat tattcttaat ttgtttaatt ttttccttgg tgggaaaagt
     1081 agttaacagg tgaagatcag ccaaaaccaa aaatacttgg tactgatcct gatatttaac
     1141 tcgattttgg agagaaccaa cccaatgacc aagatgtaac ttacccgttg gtcgatcccc
     1201 cgttaaaatt cgtaatttag tccccggcaa cataatgata taataccacc tatggctcag
     1261 atcactcacc aaataaaagc ggaaataaga acagctaaag gaagaagcca ggtaaaacat
     1321 ctaaaagagg cgggattaat cccggcaacg ctttatggta aaggcatagc ttcaatttcg
     1381 attcaatttc cagctaaaga acttataaaa acttttaaag aggcaggcga atcggtttta
     1441 gttgaattac tacttgataa tcaaaagata cctgttcttt tcaagaatcc acagtatcac
     1501 tatctaaatg gccatttaat tcacctggat tgtttccgag ttaatttaaa ggaaaaaatt
     1561 accgccgaag tgccaatcgt cctggttggc gaaagtccgg cagtcaaaga aggtaatatt
     1621 ttggtcactg ttagtgatca aatcaatgtc gaagctcttc cggctgactt accggaaaaa
     1681 attgaggtcg atatcggttc tttaactacc atcggggcta taatcaccgc cggcgatcta
     1741 attctcgata aatcaaaagt tgaactaaaa acggctgctg accaagttat agttaaaatc
     1801 gaagagccga aaattgaag
//
LOCUS       DAOOIE010000111         1809 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_52130_length_1809_cov_2.235462, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000111 DAOOIE010000000
VERSION     DAOOIE010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52130_length_1809_cov_2.235462"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="PK131_02915"
     CDS             <1..501
                     /locus_tag="PK131_02915"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45103.1"
                     /translation="PCFGNEMPKLPFHGPARDEKWTIADTYGENAEIVYSYKKWRIKQ
                     IFKLEKVFVHQFIIENKGMRKLPVNLAVHYYFNLNYKDLTINGDKMEQYIKADGEIQA
                     KRTNIIDDGTIRILMILDGFERIHLWTGRKNGIYDESYCCLEPLWGLRQIQPREKANL
                     TVKLRV"
     gene            complement(491..1471)
                     /locus_tag="PK131_02920"
     CDS             complement(491..1471)
                     /locus_tag="PK131_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45104.1"
                     /translation="MLKDLALALILGVVVGFGLTGGFFTWHNRSKTTQISPTPIPQPA
                     TFELNLTPSPQFSVNNMSLNILEPDNNALVNTTKIKITGQSSPQARIIINTPTDVLDT
                     TADEEGNFTSTVNLEIGLNQIQVTAIDSNDNQNETNLTITYSTNKFLPYPSTSTSDDK
                     TQELRDISKQKAIDSLQKAFWGNITQINNSQITIKQNDTDYQLTVDDNTVVVNETGNK
                     IKLNSLKTNQTILAMGFYDPTTNTLTTKRINLIDSQIIEKKYQVVVGNIADISQETSI
                     LTIIPTKNKEMQYQVKNNNKDLKIGDKIIVSLISDPKTANTYLALHLIKL"
     gene            1516..>1809
                     /locus_tag="PK131_02925"
     CDS             1516..>1809
                     /locus_tag="PK131_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45105.1"
                     /translation="MEIKYLGSRTVWLKGKKEVVVINPEENKIPVDCRIVVVTEKNSS
                     YLGQDEKKVVVKGPGEYEIGGVEIKGWPVGKKGVIYRLDLDGLAIGVIGQLEEI"
BASE COUNT          579 a          223 c          406 g          601 t
ORIGIN      
        1 ccttgttttg gtaatgaaat gcctaaatta ccctttcatg gtccggccag agatgaaaaa
       61 tggactatag ccgataccta cggtgaaaat gccgaaatag tttatagcta taagaaatgg
      121 cgaatcaaac agatttttaa attagaaaaa gtttttgtcc atcaatttat aattgaaaac
      181 aaagggatga ggaaattgcc agtcaattta gctgttcatt attattttaa tttaaattat
      241 aaagatttga caataaatgg ggataaaatg gaacaatata ttaaagctga tggagagatt
      301 caagccaaga gaactaatat tattgacgat ggcacgatta gaattttaat gattttagac
      361 ggttttgaaa ggattcattt gtggacaggt cgaaagaacg gaatttatga tgagtcatac
      421 tgttgtttgg aaccgttgtg gggacttcga caaatccaac cgcgggaaaa ggccaatttg
      481 acagtaaaac ttagagtttg attagatgta aagccaaata ggtattagct gttttgggat
      541 cagagataag agaaacaata attttatcac ctatttttag gtccttatta ttgtttttta
      601 cttggtattg catttcttta tttttagtcg gaattatagt taatattgaa gtttcttggg
      661 aaatgtcggc aatattaccg actactacct gatatttttt ttcaataatc tgagaatcaa
      721 ttaaattaat tcttttagtt gttaaagtat tagtggtagg atcatagaaa cccatcgcta
      781 aaatagtctg attggttttg agagaattta atttaatttt attgcctgtt tcgttgacaa
      841 ctactgtgtt atcatcaaca gttaattgat aatcagtatc attctgtttg atggtgattt
      901 gagaattgtt gatttgagtg atattacccc agaaagcttt ttgtaaacta tcaattgctt
      961 tttgtttaga aatatcacgg agttcttggg ttttgtcatc ggaagtagaa gtgctgggat
     1021 aaggcaggaa tttattagtc gagtaggtaa tagttaaatt agtttcattt tgattatcat
     1081 tactgtcaat agccgtgact tgaatttgat taaggcctat ctctagatta actgttgaag
     1141 taaaattacc ctcctcatcg gcagtcgtgt ctaagacgtc tgtgggggtg ttgataataa
     1201 ttcgggcttg aggagagctt tgaccggtga ttttaatctt ggtggtgtta actagggcgt
     1261 tattatctgg ttcgaggata tttagggaca tgttgttaac tgaaaattga ggtgaagggg
     1321 ttaagtttaa ctcgaaggtt gctggttggg ggataggagt gggagaaatt tgagtagtct
     1381 ttgagcggtt gtgccaagtg aaaaatcctc cggttaaacc gaatcctacc accactccta
     1441 aaattaaagc taaagctaaa tctttcaaca tttaaaacga ttttaccata gagcttaatg
     1501 ataaaattag acaatatgga aattaaatat ttaggttctc ggacagtttg gttaaagggt
     1561 aaaaaagaag tagttgtaat caatcctgaa gaaaataaaa tcccggttga ctgccggata
     1621 gtggttgtta ccgaaaagaa tagcagttat ttaggacagg atgagaagaa agtagtagta
     1681 aaaggaccgg gcgagtatga gattggtgga gtggaaatta agggttggcc cgtcggtaaa
     1741 aagggggtaa tttatcgtct ggatttagat ggtttagcta taggcgttat cggtcaatta
     1801 gaagaaata
//
LOCUS       DAOOIE010000112         1801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_52467_length_1801_cov_2.939863, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000112 DAOOIE010000000
VERSION     DAOOIE010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52467_length_1801_cov_2.939863"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PK131_02930"
     CDS             complement(<1..203)
                     /locus_tag="PK131_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45106.1"
                     /translation="MKRNLKPCFKAVIFDVGGVLKLTDTKATKKKIIAQFPITLEQIN
                     QSYRQYYPAWQAGEITACSLWRQ"
     gene            complement(175..>1533)
                     /locus_tag="PK131_02935"
     CDS             complement(175..>1533)
                     /locus_tag="PK131_02935"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPD45107.1"
                     /translation="NPRNAASGLSQRLDGKYADFCSLYAVDLFPANFKTEMEKIEFIR
                     SLGIKSVDSYLCRSLVEVEAIYQQFLAKRQNYPFEIDGLVIKVNGLAIQRNLGEHDNR
                     PKGQVAYKFPSQSSETRIKNISWQVGPLGFVTPVAEVEPIELAGAIIRFASLANYDLV
                     KKLNINIGDIVEISRRGDVIPHIEKIVTKVTPGHVAVPVNCPSCQTKLRVESKALRCP
                     NLCCRAQILGILKLFCQTLEIKGLSDKTIEKLFNSGKLKLPGDFYRLTVDDIAGLENL
                     GEKSARNILNQIQTKQKLTLVQLFDAAVIPNFSTARIKQVIKAGFDTPEKILNLKVTD
                     LEGLPGFQITLAQKIIAGLSARRQVIQSILNQIKIETSTKWRTSLLNNLNFVITGELS
                     IPRKQMIDKIESLGGTVHSAVSSKTDYLLTNEKNATSSKFLAAKKLGIKIINEKEFEA
                     LL"
     assembly_gap    1534..1543
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1544..>1801)
                     /locus_tag="PK131_02940"
     CDS             complement(<1544..>1801)
                     /locus_tag="PK131_02940"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPD45108.1"
                     /translation="KCDGISLEIQYQNGRFSDAITRGDGFIGDVITQNVVKMKNFVLK
                     LKKKFTGSIRCEIMVTYNDFERLNNIVKTDRQVRPTDLYSNP"
BASE COUNT          546 a          340 c          362 g          543 t
ORIGIN      
        1 atttgccgcc acaggctgca ggccgtaatt tcgccagctt gccaagcagg atagtattgg
       61 cggtagcttt ggttaatttg ttctagagta atagggaatt gagctatgat tttcttcttg
      121 gtggctttgg tatcggttag ttttaaaacc ccgccgacat cgaaaataac ggccttaaag
      181 cagggcttca aattcctttt cattgatgat tttaattccc agttttttgg cggcaagaaa
      241 cttggatgaa gtggcatttt tctcattagt taatagataa tcagttttgg aggaaacggc
      301 tgagtgaaca gtaccgccaa gggattcaat tttgtcaatc atctgtttac gagggattga
      361 tagctcacca gtaatgacaa aattaaggtt atttaataga gacgtccgcc atttggtgga
      421 tgtttctatt tttatttgat taaggattga ttggataact tgccggcggg cagagaggcc
      481 agcgatgatt ttttgggcta gagtaatttg aaatccaggc agaccttcga gatcagttac
      541 ttttaaattt aaaatctttt cgggggtatc aaaaccggct ttaataacct gcttgattct
      601 ggcagtagaa aaattaggaa taacggcggc atcaaacaat tgaaccaaag ttagtttttg
      661 tttagtttga atctgattaa gaatattcct agctgatttt tcacccaaat tttccaaacc
      721 ggcgatatca tcgacagtca atcggtaaaa atcaccagga agttttaatt tcccggaatt
      781 aaaaagtttt tcaatggttt tatcggacag acctttaatc tctaaggttt gacaaaaaag
      841 ttttaaaata cccaaaattt gggctcggca gcagagatta ggacaccgaa gagccttcga
      901 ttcaaccctt agtttagtct gacaactagg acaattaaca ggaacagcta catggccggg
      961 tgtaaccttg gtcacaatct tttcgatatg gggaatgaca tcgccgcgac ggctgatttc
     1021 aacaatatca ccgatattaa tgtttaattt ctttaccaaa tcataattag ccaaagaggc
     1081 aaaacggata atggccccgg ccaactcaat tggttcaact tcagctaccg gagtaacaaa
     1141 acctagcggt cctacctgcc agctgatatt ttttattcga gtttctgacg actggctggg
     1201 aaatttataa gccacctggc ctttgggtcg attatcatgc tcacctaaat ttctctgaat
     1261 cgccaaaccg ttgaccttga taaccaaacc atcaatttca aaaggataat tttgccgttt
     1321 agcaagaaat tgctgataaa tagcctcaac ttcgactaga ctgcggcaga gataagaatc
     1381 aacagattta atacctaaag atctgataaa ttcaattttt tccatttcag ttttgaaatt
     1441 agccggaaag agatcaactg catagagaga gcaaaaatca gcatatttgc catcaagacg
     1501 ttgagataag ccggaagcgg cattgcgggg attnnnnnnn nnnggattgg aatataaatc
     1561 ggtagggcgg acctgtcgat ctgtctttac gatattattt aaacgttcaa aatcattata
     1621 ggtaaccata atttcgcaac ggatcgagcc ggtaaatttt tttttcaact ttaaaacgaa
     1681 atttttcatt ttaaccacat tctgagtaat tacatcgccg ataaaaccat cacctcgggt
     1741 aatggcatca ctaaaccggc cattttggta ttgaatttcc aaggaaatac catcacattt
     1801 t
//
LOCUS       DAOOIE010000113         1794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_52772_length_1794_cov_3.434733, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000113 DAOOIE010000000
VERSION     DAOOIE010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52772_length_1794_cov_3.434733"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..471)
                     /locus_tag="PK131_02945"
     CDS             complement(<1..471)
                     /locus_tag="PK131_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HPD45109.1"
                     /translation="MLKEKNWRIYDYRQMDPIKILAQKLGRKTAGNLSRLKFAEVAAS
                     RGESAGVIDCGSFYLAIVIEGLGTKNLVADQMEKLTGKTYYQAVAQDAVAMIVNDLLT
                     VGADPVAVPAYFGLGSDQWLKNKKRVEALIKGWVKACDLAGASYPGGETPVLSGI"
     gene            complement(481..1488)
                     /locus_tag="PK131_02950"
     CDS             complement(481..1488)
                     /locus_tag="PK131_02950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045550 - geranylgeranyl reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0030494 - bacteriochlorophyll biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranyl reductase family protein"
                     /protein_id="HPD45110.1"
                     /translation="MTKTYEAIVVGAGPAGSVCGYTLAKSNKRVLIIDKEKFPRPKLC
                     AGMLTEKSIKMLKTVFGRINWDEIIDSSYNTFEIYHRRYGFINRGISSRNKLYLVDRR
                     IFDNFLLQKAIQAGCDFQQKNIVDLKDLKADLIIGADGANSVVRRSFTPEINRRDYSL
                     ALEIEAEKSAIGEIKYPRIYFGYVNYGYAWVFPRRDKVVVGLGGLTAKNKENMRDLFV
                     NFLKDVLKTDYRTYRILGYPLPFHNFINRPIQDNLRLVGDAVGLADPLTGEGIYYAIE
                     SGYLAAQSIYSYQPIHRVWHRAKTMRNIFYSPLFHLLIIRWLKADQRLCRWYFDLLSG
                     V"
     gene            1516..>1794
                     /locus_tag="PK131_02955"
     CDS             1516..>1794
                     /locus_tag="PK131_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45111.1"
                     /translation="MVKCRLCGTDKIEKSFIASNYHGRHYWGQEKFQLYHCQKCGCYF
                     LDKIENQKDYYRKYYPKKYSAKATRMDEIFLFFKKKLINQHFAGQQKIS"
BASE COUNT          586 a          415 c          291 g          502 t
ORIGIN      
        1 aatgcccgat aaaaccggag tttcgccgcc gggataggaa gcaccggcta aatcacaagc
       61 tttaacccag cctttaatca aggcttcaac tcttttttta ttttttaacc attggtctga
      121 tcctaaacca aaataagccg gtacggctac tgggtcagcg ccgacagtca gcaaatcatt
      181 aacaatcatg gcaacggcat cttgggctac tgcttggtaa taagtcttgc cggtcaattt
      241 ctccatctga tcagccacta aatttttcgt ccccaaacct tcgatcacta tcgccaaata
      301 aaaagagccg caatcaatta ctccggccga ttctccccga ctggcagcga cttcagcaaa
      361 tttaagccgg ctaagatttc ctgccgtttt tctccctaac ttctgggcta aaatcttaat
      421 tggatccatt tgccgataat cataaatccg ccaattcttt tcctttagca tatagccaaa
      481 ttatacccct gacaaaagat caaaatacca acggcagagc cgctggtcag cttttaacca
      541 gcgaattatt aaaagatgga aaaggggcga ataaaaaatg tttctcattg ttttggctcg
      601 atgccaaacc cgatgaatcg gttgatagga ataaattgat tgagccgcca aatagccgct
      661 ttcaatagca taataaattc cctcgccggt taacggatca gccagaccga cggcatcacc
      721 caccagccgg agattatcct gaatcggccg gttaataaaa ttatgaaatg gcaagggata
      781 acctaaaatc cgatatgtcc gataatccgt ttttaaaaca tcctttaaaa aattaacaaa
      841 caaatctctc atattttctt tattcttggc ggttaaacca ccgaggccaa ctactacttt
      901 atctcggcgg ggaaaaaccc aagcgtaacc atagttaaca taaccgaaat aaatgcgggg
      961 gtatttaatc tccccaatgg cggatttctc cgcttcaatt tccaaagcca aagaataatc
     1021 ccttctattt atttctggtg taaaactccg tctaaccaca ctattagcgc catcagcacc
     1081 gataattaaa tcggctttta aatccttaag atcgacaata tttttctgct gaaaatcaca
     1141 accggcttgg atcgctttct gtaaaagaaa attatcaaat atcctccgat caaccaaata
     1201 taatttattt ctcgatgaaa tgccgcgatt aataaaacca tatcgccgat gataaatttc
     1261 aaaggtatta taggaagaat caataatttc atcccaattt atccggccaa aaacagtctt
     1321 taacatcttg atactttttt ctgtcaacat tccagcacaa agtttaggtc ggggaaactt
     1381 ttccttgtca ataattaaaa cccttttatt acttttagct aaagtatagc cgcaaaccga
     1441 tccagccggc ccggcaccaa caacaatcgc ctcgtaagtt ttagtcattt taactactaa
     1501 attatataat cagctatggt taaatgtcga ctatgcggca ctgataaaat agaaaaaagt
     1561 tttatcgcca gtaattatca cggccggcat tattggggtc aggaaaaatt ccaactttat
     1621 cactgtcaaa aatgcggctg ttattttttg gataaaatcg aaaaccaaaa agattattat
     1681 cgaaaatatt acccgaaaaa atattctgcc aaagccaccc gaatggatga aatattttta
     1741 tttttcaaaa agaagctaat taaccaacac tttgccgggc agcaaaaaat ctcc
//
LOCUS       DAOOIE010000114         1785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_53183_length_1785_cov_3.320231, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000114 DAOOIE010000000
VERSION     DAOOIE010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53183_length_1785_cov_3.320231"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="PK131_02960"
     CDS             <1..1187
                     /locus_tag="PK131_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPD45112.1"
                     /translation="NWNGRLVSGTLEMADKQQVINSIKESGLVPIRVVQKKDSWFTEL
                     TKRLIGRASSKQVANITRELSTMMTAGLALTDSLSLLRNQVKEGTEMYEILDYALKMV
                     QGGQSLGTALAKYKEVFGEAYVASINAGEEGGVLEEILQKLADNLEGEQEFKGKVKGA
                     MIYPVIIIVGMIIVAIIMMIFVIPKLMTMYGDFGAELPAATKFLIALSGFVSKTWFLL
                     PVLIIGLMFMLKTGQKNKSFRLKRDRLVLKLPIWGELTKKTIIANTTRTLSMLTAAGI
                     SLDDSLKIVASVAGNEVYYDAYIRIAQRVQKGFDIASSFEETGIFPAIVTQMVTTGEA
                     TGKLDDVLMRVSNFFKIEAEQSVKSLTAAIEPLIMVVLGIGVAFLIIAVLMPIYNLTS
                     QF"
     gene            1205..>1785
                     /locus_tag="PK131_02965"
     CDS             1205..>1785
                     /locus_tag="PK131_02965"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HPD45113.1"
                     /translation="MKQKAFTLVELLIVIALIAILSVAVLSTINPIEQSNKARDSRVQ
                     NDAAEVLNAYERYYSTKQKYPWEEFADAIGANDEVVLRSDTYGFGVCSDAADLATLIA
                     HNAVDAAGGCTSGAYTNFLIQTSELKSSFAGKDEFQPVIDGTGLSQAMWVARKINGDS
                     LYVCFVPKAKANRDDTAKMRCLGSDGVVYKVGEG"
BASE COUNT          497 a          295 c          467 g          526 t
ORIGIN      
        1 gaaattggaa tgggaggtta gttagcggga ccttggaaat ggctgataaa cagcaagtga
       61 ttaattcgat taaagagagt ggtttggtgc caattcgggt ggttcaaaaa aaagacagtt
      121 ggtttacaga gttaaccaaa agattgatcg gtcgagcttc atcaaaacag gtggccaata
      181 ttactcggga attatcgaca atgatgactg ccggtttagc gttgaccgat tctctgtcgc
      241 ttttacggaa tcaggttaag gaagggacgg aaatgtatga aattttagat tatgccttaa
      301 aaatggttca aggagggcag tcgctgggaa cagccttggc taaatataag gaagtttttg
      361 gtgaagctta cgtggcttcc atcaacgccg gcgaggaagg cggagttcta gaagaaattc
      421 tccaaaaatt agccgataat ttagagggtg aacaggaatt caagggtaag gtaaaaggag
      481 ccatgattta cccggttatt atcattgtcg gcatgataat cgtggcaatt attatgatga
      541 ttttcgttat tcctaaactg atgacgatgt atggtgattt tggcgctgaa ctgccggcgg
      601 caaccaaatt tctgattgct ctttccggtt ttgtttccaa aacttggttt ttactgccgg
      661 ttttgattat cggcctgatg tttatgttga aaacaggaca gaagaacaaa agttttcggc
      721 tgaaacgaga ccggctggtt ttaaaattac cgatttgggg agaactgacc aaaaaaacaa
      781 ttattgccaa tacgaccaga actttatcaa tgctgacggc cgctggtatt tctttggatg
      841 attctttgaa aattgtggct tcggtagccg gcaatgaagt ttattatgat gcttacataa
      901 gaattgccca gcgggtccaa aagggttttg atattgcttc atcgtttgaa gaaacgggta
      961 tttttccggc aattgttacc caaatggtaa ccaccggcga agcgaccggt aaattagatg
     1021 atgttttgat gcgggtatca aattttttta agattgaagc ggaacaatcg gtcaaatcat
     1081 tgacggcggc gattgaacca ttgattatgg ttgttctcgg tattggcgta gcctttttga
     1141 tcattgctgt actgatgccg atttacaact tgacaagtca attttaaagg gctttaatta
     1201 ttgcatgaaa caaaaagctt ttactttagt cgaactgtta attgttattg ctttaatcgc
     1261 cattttgtcg gtagcagtgc tgtcgacgat taatccgatt gaacaatcaa ataaagcccg
     1321 ggattcgcgg gttcagaatg atgcggccga ggtattaaat gcctatgaga ggtattattc
     1381 caccaaacag aaatatcctt gggaggaatt tgccgatgcg attggagcta acgatgaggt
     1441 ggttttacgg tccgatactt atggctttgg agtttgttcc gatgccgccg atctagcaac
     1501 gctgattgct cataatgccg tcgatgcggc cggaggttgt acttcggggg cttacactaa
     1561 tttcttaatt cagacatcag agctgaagtc gagttttgct ggtaaagacg agtttcaacc
     1621 agtgattgat ggaaccggtt tgagccaagc aatgtgggtg gcgagaaaga ttaatgggga
     1681 ctctttgtat gtttgctttg tcccaaaggc gaaagctaac agggatgata ctgccaagat
     1741 gaggtgtttg ggcagcgatg gagtggtata taaggtggga gaggg
//
LOCUS       DAOOIE010000115         1783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_53286_length_1783_cov_2.743056, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000115 DAOOIE010000000
VERSION     DAOOIE010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53286_length_1783_cov_2.743056"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="PK131_02970"
     CDS             complement(<1..952)
                     /locus_tag="PK131_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HPD45114.1"
                     /translation="MPKIHKIIIKGEKVDKIYRLEGIKKKEAVYLAEKLFCDPINQRY
                     IVDEKTEGIEVAYKPGVMNPEAASIMKAAGDLGINLLAADSSLAYKNVERKRIELNDI
                     TQHFILEEPKTLIIGSQPGPVKIIKIRGMSDDKLMEISNEKLFLSLEEMKIIKEYFEK
                     MKRNPTDVELEIIAARWSEHCGHKTFKAKIVVDGKEKLPLFTRIKQTARKYFKNKIIS
                     AFEDNSGVMRFYDGQAVCGKVETHNSPSAIEPYGGAMTGSGGVFRDPLGTGQGAKLII
                     STDMFCFAPWDLDRRLLPPGTLSPDYLQRKVVEGVRDYGNR"
     gene            complement(936..>1783)
                     /locus_tag="PK131_02975"
     CDS             complement(936..>1783)
                     /locus_tag="PK131_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HPD45115.1"
                     /translation="AKNNWEAQKAVLDLKRFGAAGKTYLIEKWLGDSNQPAEEFSIFV
                     FTDGKNYQIAGSAQDHKRALDGDKGENTGGMGCSSPPLLITPQFLKKVKTRILDKIIK
                     NLKNYQGVLYLGGIAIKEDGQLNPYVVEFNARWGDPEAEVILPGLKTDLFAISRAILK
                     RKLNNLKIKTDSRYRVAVTVAAKGYPDDYSAVKGKEIIGLDMVKNVKIYGAGVKVMNN
                     KFYAAGGRLFYVVGEGKNVLAARKRAYTALAKIAIEGNNLYYRTDIGWRDVYRLKNKF
                     HAKNS"
BASE COUNT          501 a          436 c          296 g          550 t
ORIGIN      
        1 tccgattgcc gtaatcccgc actccttcaa ccacttttct ttgcagataa tcaggagaaa
       61 gagttcccgg cggcaggagg cggcgatcca aatcccaggg agcgaaacaa aacatatcag
      121 ttgaaatgat cagtttagcc ccctggccag tgccgagagg atcgcggaaa actccgccgc
      181 tgccggtcat cgctccgcca taaggttcaa ttgccgacgg gctgttatgg gtctccactt
      241 tgccgcaaac cgcttggccg tcgtaaaacc tcatcacccc cgagttatcc tcaaaagcgg
      301 aaataatttt atttttaaaa tacttccggg ctgtttgttt aattcttgta aacaagggca
      361 atttttcttt gccatcaaca acaatcttag ccttaaaagt tttatggccg caatgctcac
      421 tccagcgggc ggcaataatt tctaattcaa catcagtcgg attacgtttc atcttctcga
      481 aatattcctt aataattttc atctcctcta aacttaaaaa caatttctca ttcgaaattt
      541 ccattaattt atcatcagac attcctctaa ttttaataat cttaaccggt ccgggctggc
      601 tgccgattat taacgtttta ggttcttcta aaataaaatg ctgggtaatg tcatttaatt
      661 caatcctttt tctctcaaca tttttataag ccaaactgga atcggcggcc agtaaattaa
      721 tccctaaatc gccggcggct ttcataattg acgccgcttc tggattcatt actcccggtt
      781 tataggcaac ttcgattccc tcggtttttt catcaacaat ataccgctgg ttaattggat
      841 cacaaaaaag tttttccgcc agatagaccg cttccttttt tttaatcccc tcgagccgat
      901 aaattttatc gactttttct cctttaataa ttatcttatg aatttttggc atgaaattta
      961 tttttcaatc gatagacatc acgccagccg atatcggttc ggtaataaag attgttaccc
     1021 tcgatggcaa ttttagccaa agccgtataa gccctctttc gggcggctaa aacattcttt
     1081 ccctcgccaa ctacataaaa taaccggccg ccggcggcat aaaacttatt attcataacc
     1141 ttaacaccag cgccataaat tttaacattt ttaaccatat ccaagccaat aatctccttt
     1201 cccttaacag ccgaataatc atccggatag cctttagccg caactgttac cgccacccga
     1261 taccgactgt cagttttaat tttaagatta tttaattttc ttttaagaat tgcccgactg
     1321 atcgcgaata aatcagtttt taaaccgggc aaaatcacct cggcttcggg atcaccccag
     1381 cgggcattaa actctacaac atagggattt aactgaccat cttctttaat ggcgatgccg
     1441 ccaagataaa gaacaccctg ataattttta agatttttaa taattttgtc cagaattctg
     1501 gttttaactt tcttaagaaa ttgaggggta attaaaagcg gcggactact gcaacccata
     1561 ccaccggtat tttcaccttt atcgccgtcg agagcgcgtt tatgatcttg ggctgaaccg
     1621 gctatttgat aatttttgcc atcagtaaaa acaaaaatag aaaattcttc agccggctga
     1681 ttactatcac ctaaccactt ttcaatcaaa taagttttac cggcggcgcc gaaacgtttt
     1741 aaatccaaaa cggccttttg agcctcccaa ttattcttag ccg
//
LOCUS       DAOOIE010000116         1706 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_56964_length_1706_cov_2.375530, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000116 DAOOIE010000000
VERSION     DAOOIE010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56964_length_1706_cov_2.375530"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..978
                     /locus_tag="PK131_02980"
     CDS             73..978
                     /locus_tag="PK131_02980"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD45116.1"
                     /translation="MAKLLKIQKTSMNSLKHAWHHIRRSPFQSLIACLVMATTFFVFT
                     SFLIISQGLGAVLDYYESKPEISLFLNDGLDKSTVESIQKQLMNYPSVREVKFTSKEK
                     ALAIYQEQNKDNPLLTEMVTSSILPASFDVSVNDPKVLETIYHDFTNKKEWVSDIIYQ
                     KDIIDQLLNWTRMIRTAGLTAVALLTLSGFGVIFIILNMKITNRKEEINISRLLGASK
                     YFVQKPFLIEGIIYGLSGGIIGTLISLGLFAYLSSELNQFFRPVVFYQPTISSFLLLL
                     SIQSLFGCVIGFISSWFGVKRFTKY"
     gene            982..>1706
                     /locus_tag="PK131_02985"
     CDS             982..>1706
                     /locus_tag="PK131_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45117.1"
                     /translation="MLRKLAVLVLLIVLFLPISARAQSETDLQKQINEYQIQINKLTA
                     EKNTLSNQIKIIDSQISLAQLKINQTENEINQLEADIQRLTGAIGDLDISLNQLTTVF
                     IQEINQNYRLLKQGPPLGYLLFNNLNGFLQQKKYVTTLQKNSQETILKMETTRTNYDL
                     QKTQKEQKQIELEDLQKKLVQQKKTLDQQKDVKKNLLAITQNSEANYQRLLAQARAEL
                     AAIEGILAGRGEETKVGSVNAGDR"
BASE COUNT          577 a          299 c          287 g          543 t
ORIGIN      
        1 ccaccatcat catggctacc cataatcttg aaattgttaa ttcattaaat aaacgaaaaa
       61 tttatcttaa agatggcgaa attgttaaag attcaaaaaa catccatgaa tagcttaaaa
      121 catgcctggc atcatatcag acggtctcct tttcaaagtt taattgcctg tctggtaatg
      181 gccactacct tttttgtttt tacttctttt ttgattatca gtcagggctt gggagcggtt
      241 ttggattatt atgagtctaa gccagaaatt tccctctttc ttaatgatgg tttagataaa
      301 tctacagttg aaagcatcca aaagcagtta atgaattatc cttcggtcag ggaagtcaaa
      361 tttaccagta aggaaaaagc cttggctatt tatcaggaac aaaataaaga taatccctta
      421 ttaaccgaaa tggttaccag cagtatttta cccgcctctt tcgatgtttc agttaatgat
      481 ccaaaagtgt tggaaacaat ttatcacgat tttaccaata agaaggaatg ggtttctgat
      541 attatttacc agaaggatat tattgaccaa ttgcttaatt ggactcgaat gattagaacg
      601 gccggattaa cggcagtggc tcttttgacc ctcagtggtt ttggtgtaat tttcattatt
      661 ttgaacatga aaattaccaa tcgaaaggaa gaaattaaca tcagccgtct tttgggtgct
      721 tccaaatatt ttgttcaaaa accgttttta atcgagggaa taatctatgg tttatcaggc
      781 ggaattatcg gcactttaat tagtttaggt ttatttgctt atttaagttc cgaattgaac
      841 caatttttta gaccggttgt tttctatcaa ccaactatta gttctttcct gctcctttta
      901 tcaatccaat cacttttcgg ttgtgttatc ggctttatct ctagttggtt cggcgtcaaa
      961 cggtttacta aatattaatt tatgctgaga aaattagctg ttctggtatt gctgatagtt
     1021 ctattcctgc caatttcagc ccgagctcaa tctgagactg atcttcaaaa acaaattaac
     1081 gaataccaaa ttcaaattaa taaattgacc gctgaaaaaa atactctttc taatcagatt
     1141 aaaattattg attctcagat tagtttagct caattaaaaa ttaaccagac tgaaaatgaa
     1201 attaaccaat tagaagctga tattcaaaga ttaaccggag ctattggcga tttagatatc
     1261 agtttaaacc aattgactac cgtttttatc caagaaatca atcagaatta tcgtttgtta
     1321 aaacaaggtc cgcctcttgg ttaccttctc tttaataatt taaacggttt tttacagcaa
     1381 aaaaagtatg ttactactct ccaaaaaaac agtcaggaaa cgattttaaa gatggaaaca
     1441 accaggacta attatgactt acaaaaaact caaaaagaac aaaaacaaat tgaattggaa
     1501 gatctgcaaa aaaaactggt tcaacaaaaa aagactcttg accagcaaaa agacgttaag
     1561 aaaaatcttt tggcaataac ccaaaattcc gaagctaatt accaaagatt gctagcccaa
     1621 gctagagctg aactagccgc tatcgaagga attctagccg gtaggggaga ggaaactaaa
     1681 gtcggcagtg tcaatgctgg tgaccg
//
LOCUS       DAOOIE010000117         1704 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_57063_length_1704_cov_2.703457, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000117 DAOOIE010000000
VERSION     DAOOIE010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57063_length_1704_cov_2.703457"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PK131_02990"
     CDS             <1..358
                     /locus_tag="PK131_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45118.1"
                     /translation="TLKLAAQRVINGSLVGIAPEGSWGKEKKWFTGIGHLLKEISSGT
                     DAYFVRVYIKGTSDLDILRLFHLTGTVLPKIEVVFDTPKKIADLLQGNPSAKEITNHE
                     EASYKSWVTSIERRTL"
     gene            complement(294..1484)
                     /locus_tag="PK131_02995"
     CDS             complement(294..1484)
                     /locus_tag="PK131_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45119.1"
                     /translation="MPIEIKNGLTEKHLDRFHKQFVNFGREELELLWVRTQQDGQGQK
                     RRMIHGLDTYAVVATTNGYSLVNIDPYVWYQMAVPAKEIKKYERKIQTNLNRSQWQKR
                     ETGTYQLDDLILRYQIKNREIKDTAAGRSWPTDYHLVELEIRSSQNKPGEKEQNRWDT
                     LARGIRSKGTRGNPQIAENPKEIEKYFPMQVELGCGPSIEAGIPPLYYFHTLYSVQEP
                     IKGTPILELNKDHFLNQIIEKPKKFFRRSGRIYATALTAPLTEFHYLLKELYDRQIIV
                     GEIITESFDGLCNLVDLKERYVRTFDNHQYFPKINFHPQARSLLVVGSHADRRKVQEQ
                     ARAKGLKIIFVDSEGFIGKNGIFTAYPLESAQDNDILFPMTAREFAARWKSPKIYNSP
                     LRGW"
     gene            1532..>1704
                     /locus_tag="PK131_03000"
     CDS             1532..>1704
                     /locus_tag="PK131_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015733448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExsB family transcriptional regulator"
                     /protein_id="HPD45120.1"
                     /translation="MLQYHQTKRFIKEKIKEIKLAVGDNLAMTALSGGVDSSVVTVLG
                     HKALGEKLSVCFI"
BASE COUNT          472 a          313 c          356 g          563 t
ORIGIN      
        1 aactttaaaa ttagctgccc agcgggtaat taatggcagt ttagtgggta ttgctccaga
       61 aggttcttgg ggtaaagaaa aaaaatggtt taccggcatt ggccacttac ttaaggaaat
      121 tagcagtggt accgatgctt attttgttcg tgtttatata aaaggtactt ctgatttaga
      181 tattttgcgt ctttttcatt taaccggcac agttttaccc aaaattgagg ttgtttttga
      241 tacgccaaag aaaatagccg atcttcttca gggtaatcct tcagctaaag aaattaccaa
      301 ccacgaagag gcgagttata aatcttgggt gacttccatc gagcggcgaa ctctctagct
      361 gtcattggaa acaagatatc gttatcttga gccgattcta aagggtaggc tgtgaaaata
      421 ccgtttttac cgataaaacc ttcagaatca acaaaaatga tttttaatcc tttggcccga
      481 gcctgctcct gaacctttct tctgtcggcg tgggaaccga cgactagaag tgacctagcc
      541 tgggggtgaa aatttatttt gggaaaatat tgatggttat caaaagtccg gacatatctt
      601 tcctttaaat cgactaaatt acaaagaccg tcaaaacttt cggtgataat ttccccaact
      661 ataatttgtc ggtcatataa ttctttaagt aagtaatgaa attcggtcaa aggagccgtc
      721 aaggcggtgg cataaattcg accggagcga cggaaaaatt tttttggttt ttctataatt
      781 tgatttaaaa aatggtcttt atttaattct aaaataggtg ttcccttaat tggctcttga
      841 acgctgtaaa gggtgtgaaa ataataaagg ggaggaatac cggcttcaat tgacggccca
      901 cagcctaatt cgacctgcat cggaaaatat ttttctattt ctttaggatt ttcggcgatt
      961 tgaggattac ctctggttcc ttttgaacga atgcctcgag ctaaggtatc ccaacgattt
     1021 tgttcttttt ctcccggctt attttgactg cttctaattt ctaattccac taagtgatag
     1081 tcggttggcc atgaccggcc ggcggccgta tccttaattt cccgattttt tatttgatat
     1141 cttagaatta aatcatctaa ttgataggtt ccagtttccc gtttttgcca ttgactacga
     1201 ttaagattag tttgaatttt tcgttcatat ttttttattt ctttggctgg tacggccatt
     1261 tgataccata cataggggtc aatgttcact aaagaataac cattagtagt agccacgact
     1321 gcataagtat ctagaccatg gatcattcgc cttttttgcc cctgcccgtc ctgttgggtg
     1381 cgcacccaaa ggagttctaa ttcctcccgg ccgaagttga caaactgctt gtgaaagcga
     1441 tcaagatgtt tttcagttaa tccattttta atttctatcg gcattatctt cgttttgata
     1501 tagattggcc tattatagaa ggcaaagatt ggtgttacaa taccatcaaa cgaaaaggtt
     1561 tattaaggaa aaaattaaag agattaagct ggcggttgga gataatctgg ccatgactgc
     1621 cttatcaggc ggggttgatt cgtctgtggt aacggttcta ggacataagg ccctaggaga
     1681 aaagctgagt gtttgtttta ttga
//
LOCUS       DAOOIE010000118         1691 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_57729_length_1691_cov_3.090465, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000118 DAOOIE010000000
VERSION     DAOOIE010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57729_length_1691_cov_3.090465"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PK131_03005"
     CDS             complement(<1..533)
                     /locus_tag="PK131_03005"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HPD45121.1"
                     /translation="MVEKRDRRSLKVILSILTAIIIVTSLIIIYAQGYRISLEQGINL
                     KATGILSVTSKPKAASVYINNHLVTATDNIVNLPPGDYDVKIVKDGYTPWQKRISLKK
                     EVVQQTDAYLFRTTPDIRSITFSGAINPVLSPTGTKIIYAVASASASKNNGLYQYDLT
                     NNLLPLSKNQPKLIAVN"
     gene            complement(590..>1691)
                     /locus_tag="PK131_03010"
     CDS             complement(590..>1691)
                     /locus_tag="PK131_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45122.1"
                     /translation="PNNPVKLTLDKRYERLSSGNSSSQPVLSEIQTRVVKRIQTMVGN
                     TNDERANTYYSIILNKIESRDTFFEDREAADYFLQNSAFFYYNPNNYQKLALDTRYNN
                     LRRADNIPSQSQGKFKDILVVRQGSNDLKEIVGYAQNFDGIYYLNGSPIEGTYNHITY
                     DFSDNYKNIAGLAYYSDQTQPIIIVDYQSDIEKTINGYIRYWGCPTNYYLALWNRTYL
                     TGNDENGSQIFDLDAKDYFINNLSKWRIYLNNGHPIGRVAYVYPNGVTGYITGEILDQ
                     SPTKSSFGLDYYREVEYQNSYHQHSHGYITLDQIRVHVASLDNQREGEITAKDLFNYN
                     NRITDLTEITFVNSAGGKSTAWVKTSSINWFN"
BASE COUNT          518 a          281 c          328 g          564 t
ORIGIN      
        1 aaattaacag cgattaattt aggctgattt ttactcaaag gaagaagatt gttggttaaa
       61 tcatattggt aaagcccatt attttttgaa gcagaggcgg aagcaactgc atagataatt
      121 ttggtaccag taggggaaag aactggatta atggcgccgc tgaaggtaat agaacgaata
      181 tctggagtag ttctaaataa ataggcatca gtttgttgaa ctacctcttt ttttaaggaa
      241 attctttttt gccacggagt ataaccgtct ttaacaatct taacatcgta atcaccaggc
      301 ggtagattaa caatgttgtc agtagctgtg acgaggtgat tattaatgta aactgaagct
      361 gctttgggtt tagaagtaac ggaaagaatt cctgttgcct tcagattaat gccttgttct
      421 agactaattc ggtagccctg agcataaata ataattaggc tggtgacgat tattattgct
      481 gttaaaatag acaaaatgac ttttaaactt cttcgatctc tcttttcaac catatcttta
      541 cttataataa taccatgatt cccactaatt taccgagttt acttggagtt taattaaacc
      601 agttgataga actagtttta acccaggcgg tagatttacc tccagcagag ttaacaaaag
      661 ttatctcggt tagatcagta attcgattat tgtaattaaa aagatcttta gctgttattt
      721 caccctcccg ttgattgtct aaactagcta catggactct aatttgatca agggtaatat
      781 agccatgact atgttgatgg tagctgtttt gatattctac ctctcgatag taatcgagac
      841 caaaactaga ttttgttggt gattgatcta agatttcacc agtaatatag ccagtaactc
      901 cgttaggata aacataagcc actcggccta tagggtggcc attattgaga taaatccgcc
      961 atttagacag attatttata aaataatcct tggcatctaa atcaaaaatt tgactgccat
     1021 tttcatcatt accggttaaa taagtgcgat tccagagggc cagatagtaa ttggtggggc
     1081 aaccccagta acgaatatag ccattaattg tcttttcgat atcgctttga taatcaacga
     1141 taataatcgg ttgtgtctgg tcgctatagt aagctaagcc ggcaatgttt ttatagttat
     1201 cagagaagtc ataagtaatg tgattgtaag taccctctat cgggctaccg tttaagtaat
     1261 aaattccatc gaaattttga gcgtaaccta ctatttcctt aagatcattg ctgccttgac
     1321 ggactactaa aatatcttta aatttgcctt gagattgaga aggaatatta tccgctcgac
     1381 gcaagttatt atatctggta tctaaggcta atttttggta attgtttgga ttatagtaaa
     1441 agaaggcgga gttttggaga aagtaatcag cagcctcccg gtcttcgaag aaagtatccc
     1501 gactttcaat tttgtttaaa ataatactgt agtaagtgtt ggctctttcg tcattagtat
     1561 tacctaccat ggtttgaatt cgtttaacaa cccgtgtttg aatttcgctt aaaactggtt
     1621 gggatgacga attacccgag gataatcttt catatctttt gtccaaggtt aacttaacgg
     1681 gattattggg a
//
LOCUS       DAOOIE010000119         1686 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_57996_length_1686_cov_3.496015, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000119 DAOOIE010000000
VERSION     DAOOIE010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57996_length_1686_cov_3.496015"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..910
                     /locus_tag="PK131_03015"
     CDS             44..910
                     /locus_tag="PK131_03015"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPD45123.1"
                     /translation="MKRVIWGIIIIILGGVFFRPRQVLAITPLPQRYFNENVILIDQT
                     IDNNVITAGGTVTIDKDTIIKGDLITIGGDIKIDGEVSGNLIAIGGKITISSTAKISG
                     YTIALGGETSISPEATLEGEQLINQFNQIKQKSQISRAIRNVLDLGGFLANIVSLIIL
                     VRLSSNKFNRVWKEKTLTWGKLILRGLWILIIPPIIIGLAISSFIGLPAGMVGLMVYL
                     ISLYLAQLMAAWALGKALTDNKIIRSHNEYLLAIVGLTLITALKHIPFVGPITIIFAE
                     LWGLGFLWLKYR"
     gene            892..1644
                     /locus_tag="PK131_03020"
     CDS             892..1644
                     /locus_tag="PK131_03020"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPD45124.1"
                     /translation="MVKVSVVIPAYNEEKLIERCLKSLSHQTMNRSNYEVIVVNNNSS
                     DKTAQIAQKYADKVILEKRKGVLFARQTGLIKAKSQIILRTDADGYVPNNWVKRGYQN
                     LVKNPDYVAFSGFYLPDNEKKTLKLISQFAINFRKLLYSLGGKTEWLTGSCSAIRKEA
                     FLKIGGYDFKADPVIEDQQGIGHRLAKLGKIGFDANWWVWYSPRRFNHAVNNKIDLFN
                     DYLLYQTLNNLYYFIFKRNPKKVLGKWQDIRE"
BASE COUNT          549 a          292 c          315 g          530 t
ORIGIN      
        1 ggttctgact tcaaatccta atcaataaca gtataataat agaatgaaaa gagttatttg
       61 gggaataata attattattc ttgggggagt attttttagg ccgcgtcaag ttttagcaat
      121 aactccttta cctcagcgat atttcaatga aaacgttatt ctaattgatc aaaccatcga
      181 caataacgtt ataactgctg gaggaacagt tactatcgat aaagatacta ttattaaggg
      241 ggatttaata actattggag gtgatattaa aattgatggc gaagtcagcg gtaatttaat
      301 tgctattgga ggtaaaataa ccatttcttc aactgctaaa attagcggtt acacaattgc
      361 cttaggcgga gaaactagta ttagtccaga ggcaactcta gagggtgaac aacttattaa
      421 ccaatttaat caaattaaac agaaaagcca aatttccaga gctattcgga atgtacttga
      481 tttaggtgga tttttagcca atatagttag tttaattatc ttagtccggc tctcgtcaaa
      541 caaatttaat cgagtttgga aagaaaaaac cctaacctgg ggtaaactta ttcttagagg
      601 attatggatt ttaattattc ccccgattat tattggccta gccataagct cttttattgg
      661 cctgccggct ggtatggtcg gtctcatggt ttatctaatc agcctttatt tagcccaact
      721 aatggctgcc tgggctttgg gtaaagcttt aacagacaat aaaattatcc gtagccataa
      781 tgaatatctt ctagcaattg ttggcctaac ccttataacc gccctaaaac atattccttt
      841 tgtcggaccc ataaccatta ttttcgctga attgtggggg ttaggatttt tatggttaaa
      901 gtatcggtag ttattccggc ctacaacgag gaaaaattaa ttgaaagatg tctcaaatcc
      961 ctatctcatc agaccatgaa tcggtcgaac tatgaagtaa tcgtagtcaa taataattcc
     1021 agtgataaaa cagctcagat tgctcaaaaa tatgctgata aagtcatttt ggaaaaaaga
     1081 aaaggagttt tatttgctcg acaaacggga ttaataaagg ccaaaagtca aattattctt
     1141 cgaactgatg ctgacggcta cgtccccaac aattgggtaa aacgaggtta tcaaaatctg
     1201 gttaaaaatc ccgattacgt tgccttttcc ggcttttact tacctgacaa cgaaaaaaaa
     1261 actttaaaat taatttctca atttgctatt aattttagaa aactgcttta ctccctgggc
     1321 ggtaaaaccg aatggttaac cggctcttgt tctgctattc gcaaagaggc tttcttaaaa
     1381 atcggcggct atgactttaa agccgatccg gtcattgagg atcaacaagg catcggccac
     1441 cgccttgcca aattaggtaa aattggtttc gacgctaatt ggtgggtttg gtattcaccc
     1501 cgaagattta atcacgccgt taataataaa atcgaccttt tcaatgacta tctgctctac
     1561 caaacactta ataatttata ttattttatt tttaaaagga atcctaaaaa ggtgttgggt
     1621 aaatggcagg atattaggga atagggtata aaattagtaa aagatattat atgtcttggc
     1681 atgact
//
LOCUS       DAOOIE010000120         1654 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_59661_length_1654_cov_3.147592, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000120 DAOOIE010000000
VERSION     DAOOIE010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1654
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59661_length_1654_cov_3.147592"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PK131_03025"
     CDS             complement(<1..235)
                     /locus_tag="PK131_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45125.1"
                     /translation="MGTPEKNQENLMRLDEIPLLGLLAKKVDKMVGQLGLYGTARELV
                     RESATKLEVKNQTSETQDILRNKPAVVVYNHPYD"
     gene            327..731
                     /locus_tag="PK131_03030"
     CDS             327..731
                     /locus_tag="PK131_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPD45126.1"
                     /translation="MTLSLMPKRWWPWEEEDEDWLTFPSTQSGLSISEDDHKVYVEAA
                     VPGIDPKNIEVTFQDGYLWIRGEKKEEEENKKKKYYRKASQSFSYRVAVPSDIDPNQE
                     PEATYKRGVMTIAFTKSQKAQPKRIAIKTIKE"
     gene            complement(786..>1654)
                     /locus_tag="PK131_03035"
     CDS             complement(786..>1654)
                     /locus_tag="PK131_03035"
                     /inference="COORDINATES: protein motif:HMM:NF015971.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HPD45127.1"
                     /translation="TFNPGAIYLDNKVHLLYRALTEDDVATLGYAASHDGIHFEERLP
                     QPVYVPREPFEQKLQPGVGSGCEDPRLTQIGDRIYMCYTAFNGRDAPRIALTWINVKD
                     FLAKQWLWAKPVLISPADLNDKDAFIFPEKVNGRYMLVHRVGYDIDYSFCPELDFKDE
                     RWFEEERWIFRRKGWWDSKKIGAAAPPIKTEKGWIMLYHGIAENGVYRVGAVLLDLTD
                     PIKIIGRTSRPLLEPKMLYEREGLVPNVVFPCGAVDLGGKLFVYYGGADRVIGVATME
                     IKELLKVLELSE"
BASE COUNT          514 a          385 c          310 g          445 t
ORIGIN      
        1 catcataagg atgattataa accactacag ctggcttatt tcgtaaaata tcttgggttt
       61 cactggtttg atttttaact tccagcttag tagcgctttc tcgaactaat tctcgagccg
      121 tcccatataa tcccaactga ccaaccattt tatccacctt cttagccaac aagcccaaaa
      181 gggggatttc gtccaatctc attaagtttt cttgattttt ctccggtgtt cccataaatc
      241 tattctacac ccattttccc ccttgacaaa ttagcagtcg atctgataga ctgctaataa
      301 ttactaattt agtaggaggt aaaaaaatga ctttaagctt aatgcctaaa agatggtggc
      361 cgtgggagga agaagatgaa gactggctta cttttccttc cacccagagt ggtctttcca
      421 tctccgaaga tgaccacaaa gtttacgtcg aggcagctgt tcctggtatc gaccctaaaa
      481 atatcgaggt aactttccaa gacggctatc tctggatccg aggagagaaa aaggaggaag
      541 aggaaaataa aaagaaaaaa tattatcgaa aagccagtca gtcattttcc tatcgagtgg
      601 ctgttcccag tgatatcgat cccaatcagg aaccggaagc cacttataaa cgtggggtaa
      661 tgaccattgc cttcactaaa tcccaaaagg ctcaaccgaa aagaatcgct atcaaaacca
      721 ttaaggaata aatagaccgg aaaaaagatc tttaaaaccc cgctacggcg gggttttaaa
      781 gggcattatt cactcaattc aagaaccttt aataattcct taatttccat agtcgctact
      841 ccaattaccc gatcggctcc accataataa acaaataatt taccgccgag atcaacagcc
      901 ccacaaggga aaaccacatt tggtaccaaa ccttctcttt cataaagcat tttaggctct
      961 aagagggggc ggctggttcg gccaataatt ttaatcggat cggttaaatc caaaagaacc
     1021 gcgccgaccc gataaacacc gttttcggca attccatggt aaagcataat ccatcctttt
     1081 tcagttttaa ttggcggagc ggcagcgcca atttttttac tatcccacca gccttttcgt
     1141 ctaaaaatcc aacgttcttc ttcaaaccat ctttcatctt taaaatccaa ttctggacaa
     1201 aatgaatagt caatatcata gccgacccga tgaaccaaca tataccggcc attgactttt
     1261 tcgggaaaaa taaaggcatc tttatcattt aaatcagccg gggaaattaa aaccggctta
     1321 gcccaaagcc attgtttagc taaaaaatct tttacattga tccaggttaa agctatccgg
     1381 ggagcatctc taccgttaaa ggcggtataa cacatataaa ttctgtcgcc gatctgagtc
     1441 agtcggggat cctcacagcc agaaccgact ccgggctgaa gtttttgctc aaatggctct
     1501 cgcggcacat aaaccggttg aggtaatctt tcctcaaaat gaatgccgtc atgactggcg
     1561 gcataaccca gggtagctac atcatcttca gtcaaagctc gatagagaag gtgaactttg
     1621 ttgtcgagat aaatagcgcc gggattaaaa gtgg
//
LOCUS       DAOOIE010000121         1642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_60307_length_1642_cov_3.238815, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000121 DAOOIE010000000
VERSION     DAOOIE010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60307_length_1642_cov_3.238815"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="PK131_03040"
     CDS             <1..395
                     /locus_tag="PK131_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45128.1"
                     /translation="ELSAVQELTMTVFVIPSAKKDEIIYSLIETKDDQADYQQMVYQL
                     KFTEEKSDEQRAIGQLTITGSVAPKLDLESIKSKIVGRTWSSALNYFKNNCDYFYSWK
                     IRSNLPMIGKVFPLPIIKSRINLVSRIN"
     gene            395..1114
                     /locus_tag="PK131_03045"
     CDS             395..1114
                     /locus_tag="PK131_03045"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HPD45129.1"
                     /translation="MAYVYLKKKPAKKSKPAAKNYLSFIFLVIGILSFLFVLYPLTYF
                     QWGYSRRFQPILSPLSVKFYNNEEGIMAEAVDFTQLSNWFVQNNGQTAAYSQELLSET
                     DHRSYLLSIPKLGIKDVEVTIGGKDLKKSIIHYPQTALPGQRGNAVIFGHSVLPQFFN
                     PKSYLTVFSTLFKLIPGDQIIVYFDRVHYTYLVEDVFEVKATDFSILEQRYDGRYLSL
                     VTCSPPGTYLRRLVVRARIVE"
     gene            1132..1500
                     /gene="ruvX"
                     /locus_tag="PK131_03050"
     CDS             1132..1500
                     /gene="ruvX"
                     /locus_tag="PK131_03050"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPD45130.1"
                     /translation="MNYLAIDYGLKRIGLAVAIKGIISPILPLKNDKNLFNNLRRLVR
                     DYEIDQIYLGLSEGEVAAQTRKFGDKLATMLKLKVEFIEEAVSTIEADQIYINNKRPK
                     KEYKSWIDSIAAAVILRRAI"
BASE COUNT          503 a          273 c          320 g          546 t
ORIGIN      
        1 atgaattatc ggcagttcag gaattaacaa tgactgtttt tgttattcct tcggccaaaa
       61 aggatgaaat tatttattct ttaattgaaa ccaaagatga tcaagctgac taccagcaga
      121 tggtttacca attgaaattt acggaagaaa aaagcgatga acagagagcg attggtcagt
      181 taactattac cggttcagtt gcccctaaat tagatctaga gagcataaaa tcaaaaattg
      241 ttggtcgaac ctggagttcc gccctaaact attttaaaaa taattgcgat tatttttata
      301 gttggaaaat tcggtctaat ttaccaatga ttggtaaggt gtttcctttg ccaattatta
      361 agagtcggat taatcttgtt tctagaataa attaatggct tacgtttatt tgaaaaaaaa
      421 acctgccaaa aaatctaaac cagcggctaa aaattatttg tcttttattt ttttagtcat
      481 tggtatttta tcttttcttt tcgttcttta cccgttaacg tattttcagt ggggttacag
      541 ccgtcgcttc caacctattt taagtccact ttccgtcaaa ttttataaca atgaagaagg
      601 cattatggct gaagcggttg attttactca gctttccaat tggtttgttc aaaacaatgg
      661 tcaaacagcg gcctattcac aggagctttt aagcgaaact gatcatcgaa gttatctttt
      721 atctattcct aaattaggga taaaagatgt cgaagtaact atcggtggta aggatttaaa
      781 gaaaagcatt atccattatc cccaaacagc tctaccaggt caaagaggta atgcagttat
      841 ttttggccat tcggttttac cgcagttttt caatcccaaa tcttatttga cagtgttttc
      901 aactttattt aaattaattc caggcgatca gatcatcgtt tattttgacc gagtccacta
      961 tacttatcta gtagaagatg tttttgaagt gaaagctacc gacttttcta ttttagaaca
     1021 gcgctatgat ggtcgttatc tttccttagt tacttgttca ccgccgggga cttatcttcg
     1081 ccggctggta gttagggcga gaatcgtcga atgatttgcc ttatttacta aatgaattat
     1141 ttggctattg attatggttt aaaacgaatt ggattggcag tagcaattaa gggaattatt
     1201 tcaccaatat taccgcttaa aaacgacaaa aatcttttca ataatctccg ccggttggtt
     1261 cgggactatg aaattgatca aatatacctt ggtttaagtg agggtgaagt agctgcccag
     1321 accagaaaat ttggtgacaa actggcgact atgttaaaat taaaggtcga gtttattgaa
     1381 gaagcagttt cgacaattga agctgaccag atttatatta ataataaaag gccaaagaaa
     1441 gaatataagt cttggattga ttcaattgcg gccgctgtta ttttaaggag ggcgatatga
     1501 taaaaaattt aattgctccg attcaggcct atctattatc tcaaggccgg tgtgttggct
     1561 gcggtgcttc cctcaaaaaa ggggtggaaa aaaaagtccg tggtcaaata acggttactt
     1621 gtcgttgcgg ccggatttat at
//
LOCUS       DAOOIE010000122         1590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_63342_length_1590_cov_2.875570, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000122 DAOOIE010000000
VERSION     DAOOIE010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63342_length_1590_cov_2.875570"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="PK131_03055"
     CDS             complement(<1..768)
                     /locus_tag="PK131_03055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPD45131.1"
                     /translation="MAFKRKISLPAARQAFILILLLAAFLRLYQLDKFPVSLNWDEIS
                     HSYNAYSILKTGKDQWGTKMPIFNFRAYGDYPTTLNMYLTIPWVKFLGLNAWSTRLPS
                     AICGIVIVIFAYLITESLLKNKLISLSVMFLAAISPWTLFTSRAAFQSTVAQAFLLIG
                     IYFLINFLFNQKRTLFASLIFFNLSMYGYHSSRIFVPLFLLAILIVYFKPIKKNFFAF
                     IIGILLMLPIFLNFFNPESRARSTWVSILNPSAINTIN"
     gene            complement(747..>1590)
                     /locus_tag="PK131_03060"
     CDS             complement(747..>1590)
                     /locus_tag="PK131_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45132.1"
                     /translation="TTMSTMTQDYFNYTNHFTTLHQLFISRSWGYGASLWGPNDDMAF
                     SVGWLQWLIPLITLIVLILTQKNSKPFLFFSFFALFFLFLTHNKSTFIWQTFPFMAYF
                     QFPWRFLGIAVFCLSLASSFLPLRQWLAIIFIILTIITNFNYFKEDIWYQNYNLPITK
                     VSGEGLKDYWPKYGQNFPTEYIANPLYTKKSNQVTTDLNLTQKTDLLLPVVYFPNMKL
                     FINQQEHPYTIDSHYGQVKTTLNPGSYNLKLIFYNTPIRTIANFISLLALFFLPYLWR
                     LKEK"
BASE COUNT          585 a          216 c          368 g          421 t
ORIGIN      
        1 attgatagtg ttgatggcgc ttggatttaa gatagagacc caggtcgatc tggcccggga
       61 ttcggggtta aagaaattaa ggaaaatggg aagcatcagt agaatgccga taataaaggc
      121 aaaaaaattt tttttaatcg gtttaaaata aacaatcaat attgctagta agaaaagcgg
      181 aacaaaaatg cggctgctat gatagccata catagaaaga ttaaaaaaga taagagaagc
      241 aaagagggtt cttttttggt tgaataaaaa attaattaaa aaataaatgc cgatgaggag
      301 aaaggcctga gccacagtcg actgaaaagc cgctcgggaa gtgaataaag tccaggggga
      361 aatagccgcc aaaaacatga ccgataaaga gatcaatttg tttttcaaga gtgattcggt
      421 gataagatag gcaaaaataa cgatgactat gccgcagata gccgagggca atctggtaga
      481 ccaggcattt aaacctagga atttgaccca gggaatagtg agatacatat ttaaggtagt
      541 tgggtaatca ccataagctc ggaagttaaa aattggcatc tttgtccccc attgatcttt
      601 accggttttt aaaatggaat aagcattgta gctatgactg atttcatccc aattaaggct
      661 gacagggaat ttatccaatt gataaagcct taaaaaagcg gctaaaagta gaattagaat
      721 aaaagcttgc ctggcggcag gcaggcttat ttttctttta aacgccatag gtatggcaaa
      781 aagaataatg ctagcaaact gataaaattg gcgatggtac ggatgggggt gttgtaaaag
      841 atcaatttta gattataact gccgggatta agagtagttt ttacttggcc ataatgagaa
      901 tcgatggtat agggatgctc ttgttgattg ataaaaagtt tcatattagg gaaataaacc
      961 acgggcagga gtagatcggt tttttgagta agatttaaat cggtggtgac ttggttggat
     1021 tttttggtgt acaaggggtt agctatatat tcagttggaa aattttggcc gtatttcggc
     1081 caataatctt ttaatccttc accgctaacc ttagtaattg gtaaattata attttgatac
     1141 caaatatctt ctttaaaata attaaaatta gtaataatcg ttaagattat aaaaataata
     1201 gccagccatt gacgaagagg aagaaaggag ctggctagag aaagacagaa aacagctatt
     1261 cctaagaatc gccaaggaaa ctgaaaatag gccataaaag gaaatgtctg ccaaataaaa
     1321 gtcgatttat tgtgagttag aaagagaaaa aataaagcaa aaaaagaaaa gaaaagaaaa
     1381 ggtttggaat ttttttgggt taaaattaaa acaatcaagg tgattaaggg gataagccat
     1441 tggagccagc cgactgaaaa ggccatatcg tcgtttggcc cccagagaga agccccgtaa
     1501 ccccaggatc gggagataaa taattgatga agagtcgtaa agtgattggt gtaattaaaa
     1561 taatcctgag tcatagttga catagtggtt
//
LOCUS       DAOOIE010000123         1574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_64275_length_1574_cov_3.249506, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000123 DAOOIE010000000
VERSION     DAOOIE010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64275_length_1574_cov_3.249506"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1482
                     /locus_tag="PK131_03065"
     CDS             <1..1482
                     /locus_tag="PK131_03065"
                     /inference="COORDINATES: protein motif:HMM:NF021897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HPD45133.1"
                     /translation="RGNYLKKYSPSFLKRKNLPQSALNRRFNPSLKHQILNVEELAAL
                     WHFPGPLNKLPNIAWGKKLYADPPENLPTAENLSLEEKQKITFFAKTEFHNKETVFGI
                     REGEDRRRHTYIIGKSGTGKTTLIANMAVDDIRKGRGVAIIDPHGDLCNIVLDYIPAN
                     RINDCCYFNPADPKYVYPLNVLEAQNDSQKELVASGVISIFKKLYGSTSWGPRLEHIL
                     RNTLLTLVNTPNSDMTHIIEILTNKNFRDRVVAQLNNQTIKNFWINEFDRMDPKFQQE
                     SISPILNKVGQFVASTNIRNTIAHAKSKINIADIMDSKKILIADLSSGKLGEDNSALL
                     GAMLITQIQLSAMNRIFQSTEERTDFYLYVDEFQNFATEAFIKILSEARKFRLNLIVA
                     NQYMAQLDHNVQDAIFGNVGSIISFIVGNQDAYVLSKEFGSNFPPEDLVKIGRYQILC
                     KLSIDSETTAPFYATTLPPLSCKNQQRDKVIRISQERWGKNRQ"
BASE COUNT          507 a          325 c          263 g          479 t
ORIGIN      
        1 cgaggcaatt atctcaaaaa atattctccc agctttttaa aacgcaaaaa tctacctcaa
       61 tcagctctta atcgccgttt taaccccagt ttaaaacatc aaattttaaa tgttgaagaa
      121 ttagccgctc tttggcattt tcccgggcct ttaaacaagc ttcccaacat tgcttggggc
      181 aaaaaacttt atgctgaccc accagaaaat ttacctacgg ccgaaaattt aagtctagag
      241 gaaaaacaaa aaattacttt ttttgctaaa accgaatttc acaataaaga aaccgttttt
      301 ggaatcagag agggtgaaga ccgtcgccgt cacacttata tcattggtaa atccggtacc
      361 ggcaaaacta ccctcattgc caatatggct gtagatgata tcagaaaagg acgaggggtc
      421 gcgattattg atcctcatgg tgatctatgc aatatagtcc tcgattatat tcccgccaat
      481 agaattaatg attgctgtta ttttaatccg gccgatccaa aatatgttta tcctcttaat
      541 gttcttgaag ctcaaaatga ttctcaaaaa gagctggtag cttctggtgt tatttcgatt
      601 tttaaaaagc tctacggcag tacctcttgg gggccgcggt tagagcatat tttacgtaat
      661 actctattga cattagtaaa tactcctaat tctgatatga cccatattat cgaaatttta
      721 accaataaaa atttccgtga ccgagtagta gcccaactaa ataatcaaac aataaaaaac
      781 ttttggatta atgagtttga tcgaatggac ccaaaatttc aacaagaatc tatctcgcca
      841 attctaaata aagtcggtca atttgtcgcc tcaaccaata ttagaaacac tattgctcac
      901 gctaaatcaa aaatcaatat tgctgatatt atggacagta aaaaaatcct cattgctgac
      961 ttgtcttctg gtaaattagg tgaggataat tcagctctcc tgggagctat gttaattact
     1021 caaattcagc tctcagctat gaatcgtatt ttccaatcga ctgaggagcg aaccgatttt
     1081 tatctctacg ttgatgaatt ccaaaacttt gctactgaag ctttcattaa aattctttcc
     1141 gaagcccgca aatttagact caatctcata gttgctaacc aatatatggc tcagcttgac
     1201 cataatgtcc aagatgctat ttttggtaat gtcggctcaa ttatttcttt cattgtcggt
     1261 aatcaggatg cctatgtcct ctcaaaagaa ttcggttcta actttccacc ggaagactta
     1321 gtcaaaatcg gccgttacca aatcttgtgt aaattaagca ttgattccga aactactgcc
     1381 cctttttatg ctactaccct accaccatta agctgtaaaa accagcagcg ggataaagtt
     1441 atcagaattt cacaggaacg ttggggaaaa aataggcagt agaatttact tatgagaaaa
     1501 ccccttttat taaaaggagg gttgtctatc tatgatccaa tttccaactg gcccagttac
     1561 aggcaggata atga
//
LOCUS       DAOOIE010000124         1559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_65188_length_1559_cov_2.570479, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000124 DAOOIE010000000
VERSION     DAOOIE010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65188_length_1559_cov_2.570479"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1559
                     /locus_tag="PK131_03070"
     CDS             <1..>1559
                     /locus_tag="PK131_03070"
                     /inference="COORDINATES: protein motif:HMM:NF019880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPD45134.1"
                     /translation="DLFTFLQEFEKIDFREALETAAKMAGVTLTSGVGFDKAGRRRQH
                     LLWLNKETAIFFHYILLSHPLGRNCLKYLKDRQIAVKTMKEFVLGFAPADDNLLIHFL
                     RKKGFTDQDLVDGGCFLRGQSNSLYNRFKNRLIFPLFNHRGEICGFSGRILPGAAQDQ
                     AKYINSPETEIYHKSELVYGLNLSQNFIRQTRTCLVTEGEFDMILPYQAGIKNGVAVK
                     GTAFTEDQLRLLYRFSDTLILALDTDFAGNTAILRSIELADKMGFDLKVLDLSPRFKD
                     PDEAVRGDLAFFQDRLAHCLSVGDFVINWAVKLYGVDSVRGKKQVLDLSLPVIAKISN
                     TVIRSDYLKKLADVLNSEPEAVFQEFQKRLSLSTLPVTTSAIDVHRPADISQTNLKEK
                     LEERLLALILLTKSPRLVARRVSKQYQFTTRRFRSLLEKLLVVDKYSPATFQSQLSSE
                     LTGVFQNVYLESHNFDFDTYRLNLEIKKLINQLTIIEVKEQLRQLAAEITQLEKNDPD
                     NRQKTLEIKYN"
BASE COUNT          450 a          306 c          322 g          481 t
ORIGIN      
        1 gggatttatt taccttttta caggaatttg aaaaaatcga ttttagagag gccttggaaa
       61 ccgcagctaa aatggccgga gtaactctga ctagcggggt cggattcgat aaggccggcc
      121 gccggcgcca gcatctttta tggttaaata aagagaccgc tattttcttt cactatatcc
      181 ttttatccca tcctttagga agaaattgtt taaaatattt aaaagatcga cagatagcgg
      241 ttaaaaccat gaaagaattt gttttaggct ttgccccggc ggatgataat ttattaatcc
      301 attttttaag aaaaaagggg tttactgacc aggatttagt ggatggcggc tgttttctgc
      361 gaggtcaaag caatagcctt tataatcggt ttaaaaatcg tcttattttt cctttattta
      421 atcaccgggg cgagatttgt ggtttttccg gccgaattct acccggcgcc gcccaggatc
      481 aggccaaata tattaattcg ccggaaactg aaatttatca caaaagtgaa ttggtttatg
      541 gtcttaactt aagccagaat tttattcgac aaacccggac ctgtttagtt accgaaggtg
      601 agtttgatat gattttgcct tatcaggccg gtattaaaaa tggagtagcg gtcaaaggga
      661 cagcttttac tgaagatcaa ttgcggctgc tttatcggtt cagcgacacc cttattttag
      721 ccctcgatac ggattttgca ggtaataccg cgattttacg tagtattgaa ctggccgata
      781 aaatgggttt cgacttgaag gttttagacc tttctcctag atttaaagat cccgacgagg
      841 cagtcagagg cgatttagcc ttttttcaag accgactggc ccactgttta tctgtcggtg
      901 atttcgtgat taattgggcg gttaaacttt atggtgtcga ttcggttagg ggtaaaaaac
      961 aagttttaga cctttctctg cctgttattg ccaaaataag caatacagtc attcgttcgg
     1021 attatttaaa aaaattagcc gatgttttaa attccgaacc ggaggctgtt tttcaggaat
     1081 ttcagaaacg gctgtcgctt tcaactttac ctgttaccac ttccgcgatt gatgtccacc
     1141 ggccggcgga catcagtcaa actaatttaa aagaaaaact ggaagaaaga ttactcgcct
     1201 taatcctgct gaccaaatcc ccccgactag ttgcccggcg ggtttctaaa caatatcagt
     1261 ttaccacccg gcgttttcgg agtttactag agaagctatt agtcgttgat aaatattccc
     1321 cagccacttt tcagtctcaa ctttcctctg aactgaccgg tgttttccaa aacgtttatt
     1381 tggaatcaca taatttcgat ttcgatactt atcggcttaa tttggaaatt aaaaaattaa
     1441 ttaatcaatt aacaattata gaagttaagg aacaattacg tcaattagcg gccgagatca
     1501 cccagttgga aaagaatgac ccggataacc gccaaaaaac cttagaaata aagtataat
//
LOCUS       DAOOIE010000125         1557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_65291_length_1557_cov_4.531957, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000125 DAOOIE010000000
VERSION     DAOOIE010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65291_length_1557_cov_4.531957"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1366)
                     /locus_tag="PK131_03075"
     CDS             complement(<1..1366)
                     /locus_tag="PK131_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45135.1"
                     /translation="MTIEKGSISAKAISAGVSLALSLGIATHSKEAKADDTLKQLNPI
                     TTALDMYRGNHWNQSVIADYLDVLQLEDKDKYLYLGIPNYIGLAGDASLPENADLHHK
                     IYDVKTVPTWIINGILYRYSKDTAGLQLIPEGISTVKQYARFEIGSEKENVYTLKITG
                     TNEELNKRANINLNLIVFKNEVNCTNNQDLFRTTTLKRVAYKYPLGALGERIKVENGQ
                     IVVRNLGLINIPKAIQNQCQMVAFLQDMKTKEILAAGFTEMVDGETALFNWDDLPECT
                     LENVQDVLNVKGVDWAGLELKNSPQYLDKTGLKEKVFYVQKAKDLKYLSLQLDYTLDE
                     AQIYQVLAAGLSPELKDKAIFKYDPKKNQVDITFTEPINGDRKLFSVFIKINKPTVDG
                     IKDEKSFAVNKIINSVNFKMRNFYANDSQNNSLKYQLREQKNYFPVRMCTIENPFDLK
                     KNANI"
BASE COUNT          401 a          289 c          335 g          532 t
ORIGIN      
        1 caatattggc atttttcttc agatcaaacg ggttttcaat ggtgcacatt cttactggaa
       61 aatagttttt ctgctctctt agctggtatt tgagagagtt gttttgggaa tcattggcat
      121 aaaagtttct cattttgaaa ttaacactgt tgatgatttt gtttaccgca aaggattttt
      181 catctttgat gccatcaaca gtaggcttgt taatcttgat gaaaaccgag aacagttttc
      241 tgtccccgtt gatcggttca gtgaaagtga tatcgacttg gtttttcttg ggatcatact
      301 tgaaaatagc tttatctttc agttcggggc taaggccagc agccaaaacc tggtaaatct
      361 gggcttcgtc gagggtgtaa tccaactgca ggcttaaata cttcaggtct ttggcttttt
      421 gaacgtaaaa gactttttcc ttgagaccgg ttttgtctaa atattgaggg gaattcttta
      481 attctaaacc tgcccagtct acccccttta catttaaaac atcttggaca ttttccagag
      541 tacattcagg taaatcgtcc cagttaaaca gggccgtttc gccatcaacc atctcggtga
      601 agccggcggc taaaatttct ttggttttca tatcctggag gaaagccacc atttggcatt
      661 ggttctggat ggctttggga atattaatta agcccaggtt gcggacgacg atttggccgt
      721 tttctacctt gatcctttcg cccaaggcgc ccaaaggata tttgtaagcg actcttttaa
      781 gggtagtagt tctgaataaa tcttggttat tagtacagtt aacctcgttc ttgaaaacga
      841 tcaggttgag gttaatattg gctcttttgt tcagttcttc attggtaccg gtgattttga
      901 gagtataaac attttccttc tccgagccaa tctcgaatcg agcgtattgt ttgacggtgc
      961 tgatgccttc gggaatgagc tgaagaccag cagtatcttt tgaatatcgg tacaaaatac
     1021 cattaatgat ccaagtggga acagttttga cgtcgtatat cttgtggtgt aaatctgcat
     1081 tttccggtaa agacgcatcg cccgcaaggc cgatatagtt gggtatacct aggtataaat
     1141 atttgtcttt gtcttcaagt tgaaggacat cgaggtaatc ggcaatgacg ctttggttcc
     1201 agtggttgcc tctgtacatg tccagggccg tagtaatcgg gttcagttgt ttgagagtgt
     1261 catcagcttt ggcttctttg gaatgggtag cgatgcctag actaagcgct aagctgacgc
     1321 cggccgagat cgctttggcg ctgattgagc ctttttctat agtcatagtt taattatagc
     1381 ataaaactgt ctagttgtca aatactatag gatagtagct aattacgaat taccaattac
     1441 gaattacgaa ttaaaaatta cgaattacga attactaatt actatttact aattactaat
     1501 tgcttgttat tataaattat tgatgaaaaa gctgtctctt ttggctttaa tcttcct
//
LOCUS       DAOOIE010000126         1543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_66259_length_1543_cov_2.489247, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000126 DAOOIE010000000
VERSION     DAOOIE010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66259_length_1543_cov_2.489247"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(60..662)
                     /gene="tmk"
                     /locus_tag="PK131_03080"
     CDS             complement(60..662)
                     /gene="tmk"
                     /locus_tag="PK131_03080"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPD45136.1"
                     /translation="MTEGKFIVFEGIDGCGKTSQAKLLTAFLKKKGIPAIFTYEHQRK
                     NVGELIEKVLYRQLKLDPLALQLCFIADRCDHINSFIKPQLKSGKTVICDRYYWSTVA
                     YGADQDQEWLLAINKYIRIKPDLCFWLEVDPRVSLERINRGREAKTLFEQFNFLETVN
                     RHYRWLVQHDDVPVIRIDGNQKLMTIHQEILAKLGYGSSL"
     gene            complement(721..1425)
                     /gene="thyX"
                     /locus_tag="PK131_03085"
     CDS             complement(721..1425)
                     /gene="thyX"
                     /locus_tag="PK131_03085"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009145425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HPD45137.1"
                     /translation="MIKVKLIGYTREPEQNVTAAIRQCYSAVGAKEIKKKVNQETIER
                     LIRQVLESGHTSTVEHASFTFAIEGVSRACTHQLVRHRLASYSQQSQRYVDLSKNKLN
                     YITPPDIAKNKKMLSEYKKTMAIIENRYRAMIQSGIKPEDARFILPNACETKIVMTMN
                     ARSLLNFFKERCCNRAQWEIRTLANLMLKEVKKVAPNIFQNAGPTCFNEKICWEGGRS
                     CGRWQTIKGAQLKSRI"
BASE COUNT          453 a          331 c          242 g          517 t
ORIGIN      
        1 tttcactggg aattaattga attccgtttt gttggcgtct attctctgcc tcaattaatt
       61 taaagactcg atccataccc tagtttagca agaatttcct gatgaattgt cattagtttt
      121 tggttgccat caattctaat cactgggaca tcatcatgct gaaccaacca tcgataatgt
      181 cgattaactg tttccaaaaa attaaactgt tcaaataaag tctttgcctc ccgcccccga
      241 ttaatccttt ctaaagaaac tcttggatcc acttccaacc agaaacaaag atccggctta
      301 atcctaatat acttattaat tgccaaaagc cactcctggt cttggtcggc gccgtaagca
      361 actgtcgacc aataataacg gtcacaaata acggttttac cagattttaa ttgcggcttg
      421 ataaaagaat taatgtggtc gcagcgatca gcaataaaac ataattgcaa ggccagcgga
      481 tctaacttta attgacgata taaaactttt tcaattaatt caccaacgtt tttacgctgg
      541 tgttcatagg taaatatggc cggtattccc ttttttttca gaaaggctgt caataatttt
      601 gcctgacttg tcttaccaca accatcaata ccttcaaaaa caataaattt cccttcggtc
      661 ataaaaattt acaatttttt aaattgtact tttcagtgaa gaaaaatatg accagaaaat
      721 tcatatcctc gatttgagtt gagccccttt aattgtttgc catcggccac agcttcttcc
      781 tccctcccaa caaatttttt cattaaaaca ggttggaccg gcattttgaa aaatgtttgg
      841 agctactttt tttacttctt ttaacattaa attggctaaa gttctaattt cccattgagc
      901 ccgattacaa catctttctt taaaaaaatt taataatgac cgagcattca tagtcatcac
      961 aattttagtt tcacaagcgt tgggtaaaat aaatctagca tcctccggtt taataccgga
     1021 ttgaatcatt gcccgatagc gattctcaat aattgccatt gtctttttat actcactcag
     1081 cattttttta ttcttggcta tgtctggcgg agtaatataa tttaatttat ttttagacaa
     1141 atcaacataa cgttgggact gttgagaata agaagctaaa cgatgtctca ctaactgatg
     1201 ggtacaggct cgtgataccc cttcaatagc aaaagtaaaa ctggcatgct ctaccgtcga
     1261 agtatgacca gactctaaaa cttgcctaat taatctctcg attgtttcct ggttgacctt
     1321 ctttttaatc tccttagcac caacagcgct ataacattga cggatagccg ctgtaacgtt
     1381 ctgctccggc tctcgagtgt agcctatcag tttgacttta atcatgatta ctcttattct
     1441 aactcctatg gagaaaatac tcgagtatgt tctttccctc gtcaatcgca atcttcttgg
     1501 agccatgatc ggcggcggta ttattgagca aattattgtt cct
//
LOCUS       DAOOIE010000127         1527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_67266_length_1527_cov_2.898777, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000127 DAOOIE010000000
VERSION     DAOOIE010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67266_length_1527_cov_2.898777"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="PK131_03090"
     CDS             complement(<1..351)
                     /locus_tag="PK131_03090"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPD45138.1"
                     /translation="MKFRSTLLLYALILTLIGVFFIAVSSQTEANSILTDQFFFLRQQ
                     LIWLGVGLITFFVATKIKLGWLQKFSFPLYLLTNLLLLLVLIPSLGTQALGAKRWLSL
                     GQLSIQPSEIAKITM"
     gene            complement(341..1375)
                     /locus_tag="PK131_03095"
     CDS             complement(341..1375)
                     /locus_tag="PK131_03095"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPD45139.1"
                     /translation="MNPLVELINNSGYDIYFLVVDKHLDINLPELKYFYKIYLPVNEI
                     KNSGHLLSLPSVQKFIKENSQKNSHLPAILPFKSSAKINWITNNNGWLNLSNPPALTR
                     FLENKIKFYQFCQQEKLPVIPSQIDKFNRENFNHYFQGKTGVIQTSFGWAGNSSFLVK
                     NFREVENKIPQGAIVKYSPYLRGKTYINNCCLTRFGLLQSPPARQLTGLKAYTPNPLT
                     TVGRVWPSGLSFNQITKLTELTNQFGQSLAKFHYRGYFGLDFQISQGQIFILECNPRL
                     TASFAFYHLLERKKHLNSLLYFHLLEWLDLDYHFDFKHEQARWLVPIHGRELTPRDQN
                     NQIIKKINEI"
BASE COUNT          502 a          236 c          333 g          456 t
ORIGIN      
        1 catggtaatt ttagcaattt ctgaaggttg aatacttaat tggcctaagc taagccatcg
       61 tttagctccc aaggcttggg ttcccaggga gggaattaaa accagaagga gaagcaaatt
      121 agttaataaa taaagaggaa aagaaaattt ttgaagccag ccaagtttaa ttttagtggc
      181 aacaaaaaag gtaattagac cgacccctag ccagattaat tgctgacgga gaaaaaaaaa
      241 ttgatcagtc aaaatagagt tggcttccgt ctggctggag acggcaataa aaaagacgcc
      301 aataagggtt aaaatgagag cgtaaagcaa aagagttgat ctaaatttca ttgatttttt
      361 taattatttg gttgttttga tccctaggag tcagttctcg gccatggata ggaactaacc
      421 agcgagcttg ttcatgtttg aaatcgaaat ggtaatcaag gtcaagccat tccagaagat
      481 ggaaataaag gagggaattt agatgttttt ttctctctaa aagatgataa aaagcgaaag
      541 aagcagttag gcgtggatta cattctaaaa taaagatttg tccttggctg atttggaaat
      601 ctaaaccaaa ataacccctg tagtgaaatt tagctaagct ttggccgaat tggttagtta
      661 attctgtcaa cttagtgatt tgattaaatg aaaggccgct tggccaaacc cgccccactg
      721 ttgttaaagg attaggggtg taagctttta agccggttag ttgtcgggct ggtgggcttt
      781 gaagcaggcc aaaacgagtc agacaacaat tattaatata tgttttaccc ctaagataag
      841 gagaatattt gacgatagct ccttggggaa ttttgttttc aacttctcga aaatttttaa
      901 ccaaaaagct ggaattacca gcccaaccaa aactggtttg aataacgcca gtttttccct
      961 gaaagtaatg attaaaattt tcacgattaa atttgtctat ttgggaagga ataaccggca
     1021 gtttttcctg ctggcagaat tgataaaatt taattttatt ctcaagaaag cgggtcagag
     1081 ctggcgggtt acttagatta agccagccat tattattggt aatccagtta attttagccg
     1141 aagatttaaa aggaagaata gccggtaaat gggagttttt ttgtgaattt tccttaataa
     1201 acttctgaac gctgggtaaa gaaagaagat ggccgctgtt ttttatttca ttaactggaa
     1261 gataaatttt gtaaaaatat tttaattccg gtagattaat atctaaatgt ttatcaacta
     1321 ccagaaaata aatatcataa ccggaattat tgatgagttc tactaatggg ttcatcgttg
     1381 agtgccagtt aaaagaataa ccgggaattg accagcctga tggtgctgct taaaatagcc
     1441 ggctagggcg agagcccctt caggcgaagt ccgaagaaga tgtttttgaa ggtatttctg
     1501 ggcttctatt agctcccagt tttgaac
//
LOCUS       DAOOIE010000128         1527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_67284_length_1527_cov_2.292120, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000128 DAOOIE010000000
VERSION     DAOOIE010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67284_length_1527_cov_2.292120"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1527
                     /locus_tag="PK131_03100"
     CDS             <1..>1527
                     /locus_tag="PK131_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45140.1"
                     /translation="NVMNVSSISLTEVAEGNVLHFRPMQSTLDATNYFDIKSSGNNVI
                     FNVDTINKRVGIGTATPGQALDVTGAIRASIGITTPKIQLTTGATNGYILLSDASGNG
                     TWTNPAGIGGTNYFAGNGITLTPTNVFELGSSLTRVTTIGGNYPLILANALNVGTSLT
                     VGTNLNVGTTIKSANLYTTGSVGIGTSTTSSGSQLHIYNQTAGQLTIEGGEIGGPARS
                     AIITLKGTTTGRGQGMLLVSGATESWFMGQGYATSGGFTIGYGASQAEYKPQSKFFID
                     TTGNVGIGSSTPLYKLDVVGSGNFTKLGVGGTNSSYAFNVSTNANIGTTLNTATLSLS
                     NTNVTASAAELNLLDGTTSTAGSVIYGDGSKLANTSVGVSGQILMSNGSNTPSWINSD
                     ALGIGTTYEAGSGLTKTSNVFKLGGALTENVRLNIGNTEAFFINYIDGNVGLGTTAPG
                     AKLDIIGLNGSNGTAALAVSNGYVVLPTAQKLSNTTINEDLIFYLPLNGSTNSSRGHY
                     N"
BASE COUNT          418 a          358 c          314 g          437 t
ORIGIN      
        1 aacgtgatga atgtttctag tatttctctg actgaagtgg cagaaggcaa tgttttacat
       61 ttcagaccga tgcagtccac tcttgatgcc accaattatt ttgacattaa aagttcggga
      121 aataatgtga ttttcaatgt tgacactatc aataaaagag tcggcattgg cacggccacc
      181 ccgggtcaag ctcttgatgt taccggagcc atcagggcat ctattggcat taccactccc
      241 aagattcaat taacgactgg agccaccaat ggctacattc tcctctctga tgcctctggg
      301 aatggtacct ggactaatcc ggctgggatc ggcggtacca attactttgc cggtaacggg
      361 attactttaa ctcctactaa tgtttttgaa ttaggcagca gtcttactcg ggtcactacc
      421 attggcggga attatcctct cattctcgcc aacgctttga atgtcggcac tagtctaacc
      481 gttgggacta atctcaacgt cgggacgacg attaaatcag ctaacctcta cactaccggt
      541 agtgtgggca taggcacttc tactacatca tcaggatcac agttacatat ttataatcaa
      601 accgctggac aattaactat cgaaggcggt gaaattggtg gaccagcacg atcagctatt
      661 attacattaa aaggtactac tactggtcgt ggtcaaggaa tgcttttagt ctcaggtgca
      721 accgaaagtt ggtttatggg tcaaggatat gccacaagtg gtggatttac tattggttac
      781 ggtgcatcac aagcagaata taaacctcaa tcaaaatttt ttattgatac cactggtaac
      841 gtcggtattg gttcatcaac tcccctttat aaactcgatg ttgtcggttc tggcaatttc
      901 accaaacttg gtgttggtgg tactaattcc tcatatgcct tcaatgtctc taccaatgcc
      961 aacattggca ccactctcaa cactgctacc ttatctctca gcaataccaa tgtcactgct
     1021 tctgccgctg aattaaatct actggatgga actacttcaa ctgccggttc agtcatttat
     1081 ggtgacggca gtaaattagc caacactagc gttggcgttt caggtcaaat cttaatgagc
     1141 aatggcagta atactcccag ctggatcaac agcgatgctt taggtattgg cactacttat
     1201 gaagccggtt ctggtttaac taaaacttcc aatgtcttta aacttggcgg agctctaacc
     1261 gaaaatgtca gactgaatat tggcaatact gaagctttct tcattaacta cattgatggc
     1321 aatgttggct taggaacaac agctcccgga gccaaattag acattatcgg cctcaatggc
     1381 agtaacggta cggcggctct ggcagtctcc aatggctacg tcgttctacc gacagctcaa
     1441 aaacttagca ataccaccat taatgaggat ttaatcttct atctgcctct caacggcagt
     1501 actaattcca gccgtggcca ctacaac
//
LOCUS       DAOOIE010000129         1519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_67831_length_1519_cov_3.210383, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000129 DAOOIE010000000
VERSION     DAOOIE010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67831_length_1519_cov_3.210383"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..817)
                     /gene="purQ"
                     /locus_tag="PK131_03105"
     CDS             complement(65..817)
                     /gene="purQ"
                     /locus_tag="PK131_03105"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HPD45141.1"
                     /translation="MKNPRVCLLKTDGINCDEELKFAFELAKADPKIVHINELRQKKD
                     YLKNYQILALPGGFSYGDDVASGKILAIELSSYLADQINDFVNQRQGLVLGICNGFQV
                     LVKTGLLPSGRLGKMKATLTDNDCRHFVCRPINLTVEKKHRCLFLKNFTSPVTFYVAH
                     GEGKFVAPRKELEEIEKNNLVVFRYADLNPNGSLHNIAGITDLSGRILGLMPHPERSI
                     LKTQSVNWRRNTRIKPEGLELFKQMVNYARQQ"
     gene            complement(819..>1519)
                     /locus_tag="PK131_03110"
     CDS             complement(819..>1519)
                     /locus_tag="PK131_03110"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HPD45142.1"
                     /translation="MASLDQSVEAVCDCMDVFQIPVISGKDSLSSTYRGANGSVIKIP
                     PVLCISAFGRIKNVDLTVSSDFKRIGSDVYLLGKLDDNLGGSVYAQISHFSIDSIPRV
                     DLKNLPGLFNSLYRAIKSGNILACHDVSEGGVITTVAEMCFGGDCGVDLKIKNKFSPV
                     NFLFNETAGTFIIEVKKGTEVKKLFKNLPLIKLGKTQKNKTIKIQYNQQNLFNFSVIN
                     LKAAWQKPLRKVFS"
BASE COUNT          492 a          324 c          264 g          439 t
ORIGIN      
        1 tttatgccgg gaaaggaaaa attaacaaaa gattttagaa tttcttctaa aatattccta
       61 aagattactg ctgtcgggca tagttaacca tttgtttaaa taattctaaa ccttcgggtt
      121 ttattctggt gttacgccgc caattaacgg attgggtttt taaaatcgat ctctcgggat
      181 gaggcatcag tcctaaaatc cggccgctta aatcggtaat accggcaata ttatggagag
      241 agccattggg atttaaatcg gcgtagcgaa aaacaactaa attatttttt tcaatttctt
      301 ccaattcttt tctcggagcg acaaacttcc cctcgccgtg agccacataa aaagtcaccg
      361 ggctggtaaa attcttcaga aaaagacagc ggtgcttctt ttctacggtc aaattaatcg
      421 gccggcaaac aaaatggcgg cagtcattat cggtcaacgt agccttcatt tttcctaagc
      481 ggccacttgg caaaagaccg gttttaacca acacctgaaa accattgcaa ataccaagaa
      541 ccaaaccttg cctttgatta acaaaatcat taatttgatc agctaaataa gaactcaatt
      601 cgattgccaa aatttttccc gaagcgacat cgtcgccgta agaaaagccc ccagggagag
      661 ctaaaatttg ataattcttc aagtagtcct ttttctgccg caattcatta atatgaacaa
      721 ttttaggatc agctttagcc agttcaaagg caaatttaag ctcttcatca cagttgatgc
      781 catccgtttt caaaaggcaa acacggggat ttttcatttc aggaaaaaac ctttcgaagc
      841 ggcttttgcc aagccgcttt caaattaata accgaaaaat taaataaatt ttgctgatta
      901 tactggattt taatagtttt atttttttga gttttaccca atttaattaa aggcaaattt
      961 ttaaacagtt ttttaacctc agttcccttt tttacttcaa taataaaggt accggcagtt
     1021 tcattaaaca aaaaattaac cggggagaat ttatttttta ttttcagatc gacaccgcag
     1081 tcaccaccga aacacatttc agccacagtg gtaatcactc cgccttcgga aacatcatgg
     1141 caggctaaaa tgtttcccga tttaatcgcc cgataaagag agttaaataa tcccggcaaa
     1201 tttttcagat caactcgggg aatcgaatca atcgaaaaat ggctgatctg ggcataaaca
     1261 gagccgccga gattatcatc taatttaccc agcaaataaa catcactgcc tattctctta
     1321 aaatcagagc tgacagtcag gtcaacattt tttatccggc cgaaggctga aatacataag
     1381 accggtggaa tttttatcac cgaaccattg gccccgcgat aagttgaaga caaactatct
     1441 ttaccggaaa ttaccggaat ctgaaaaaca tccatacagt cgcagacagc ctcaaccgat
     1501 tggtctaagg aagccattg
//
LOCUS       DAOOIE010000130         1516 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_68064_length_1516_cov_2.193703, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000130 DAOOIE010000000
VERSION     DAOOIE010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68064_length_1516_cov_2.193703"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..109
                     /locus_tag="PK131_03115"
     tRNA            39..109
                     /locus_tag="PK131_03115"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:71..73,aa:Ala,seq:cgc)
     gene            426..>1516
                     /locus_tag="PK131_03120"
     CDS             426..>1516
                     /locus_tag="PK131_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45143.1"
                     /translation="MEIKPRKTGLQATYIFHFTIEKKIEVHQSITLVWPPGTTINPPL
                     PEDPKEKNERLKQIIESMSIGLSPCSSCQGLPIIETDKQGFLKLTFNTHIELNPDIPG
                     YKNIYVTVPDRCGFSNPKTAGTYTYKVATAPEPEYKTTTFDIVNSQTQTPQVTIEPPI
                     YNANAQYKIEFEVGAGGWLGRGTGLIKIKFPEETKFQRPIPEIKPEHISINGKILGKA
                     PNGTKNLINFTTPVEIPDNGKVTVIFDKKAGLINPNKIQQYQLEVATSIDTEWVKSKP
                     YSIQRGVAILEVEPAKVNRIAQYKILFVLDNGKLNKGNGILIRFPKAVILPQKISSNC
                     VLVNEKPVFAANFNSSTNEAVVFPDAEIL"
BASE COUNT          541 a          296 c          257 g          422 t
ORIGIN      
        1 tgggttattt ttaacaaagc tagctcaaat atcataatgg gctcgtagtt taatggcaaa
       61 acactacatt cgcattgtag agatatgggt tcgattccca tcgagtccac acctaaaaaa
      121 ttagataaca aaaaaacaat ctataatttt cgattttaga ttagcgacca attaatcgcc
      181 gacactatta attggtttga ttttgctatc aagcaatgcc agtttgacaa aaaatcaaaa
      241 attaggtaag atgtttaaac tcttaaccgt aacgattaag agagtatttt gacaatttat
      301 taaaaggagg tgttacgatg agtaacaaaa aaagaaccaa aaacgttttg attgtcttat
      361 tcataacgat cttctacctc gtcctaaatt ttcccgcttt aacaatggga atccaaaatt
      421 tttatatgga aattaaaccg cggaagacag gccttcaagc cacctacatt tttcatttca
      481 ctatagagaa aaaaattgaa gttcatcaat ccatcacgtt agtctggcca ccgggcacca
      541 caattaatcc ccctctcccg gaagatccaa aagaaaaaaa tgagagacta aaacagatca
      601 tagagtcaat gagtattggc ctctctccgt gttcctcatg ccaaggatta cctattattg
      661 aaactgacaa acaaggattt ctcaaattaa ccttcaatac tcatattgaa ttaaatcccg
      721 atattccggg atacaaaaat atttacgtga ccgtaccaga cagatgcgga tttagcaacc
      781 ccaaaacagc tggaacttat acctataagg tagctacggc accagaacct gaatataaaa
      841 ctacaacttt tgacatagta aacagccaga ctcagactcc tcaagtaacc atcgagccac
      901 ctatttataa tgccaacgct caatacaaaa ttgaatttga ggttggagcg ggcggttggt
      961 taggtcgagg aacaggatta ataaaaatta agttcccgga agaaaccaaa ttccaaagac
     1021 ctataccaga aattaaacca gaacacatta gcattaatgg taaaatactg ggtaaagcgc
     1081 ccaatggcac caaaaaccta attaacttta ccacgccagt agaaattcct gataacggta
     1141 aggtgacggt aatttttgat aaaaaagccg ggctcattaa tccaaataaa atccaacagt
     1201 atcaattaga ggtagctaca tctattgata ctgagtgggt caaatcaaaa ccctatagta
     1261 ttcaacgtgg ggtggctatt ttggaggtag aacctgctaa agtgaaccga atcgcccaat
     1321 acaaaattct ttttgttctg gataacggaa aattaaataa gggaaatggt attttaattc
     1381 gttttcccaa ggcagttatt ttgcctcaaa aaatttcttc caattgtgtt ttagtaaacg
     1441 agaaaccagt ttttgccgcc aattttaatt cttccactaa cgaagctgtg gtttttcctg
     1501 atgcggaaat tttaat
//
LOCUS       DAOOIE010000131         1510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_68442_length_1510_cov_3.279725, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000131 DAOOIE010000000
VERSION     DAOOIE010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68442_length_1510_cov_3.279725"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..>1510
                     /locus_tag="PK131_03125"
     CDS             141..>1510
                     /locus_tag="PK131_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45144.1"
                     /translation="MRRYHLSGKAVKNLLIKQNGREWYETYISAKRCHLNLIQVRTFL
                     ADLPGIVSGLESKELNLTKLTQKYHLARQTIRKLLKENKGQEWYDRCIPSSLGCCLEK
                     KATFIQSLPEISRQLELGKLNLTKLADRYDLSKQTIKKILKEKNGLDWYGLYITTRRG
                     RPPLKEKTVGESSLTKRTILIESLPQVIRQFESGELNMNQLVKNSGLRKEIIVEILKK
                     QKGSDWYKTYVTARRGHGHSHQERKIAFSRSLDEIIPKIESRQVGVVILSKQYHLGVE
                     TIKRLLEEQKGSEWYKAYVTTKIGWRNSYWKKDFLTDLPLITQQIEAGQLRFRQIAKQ
                     YHLAEKTIRKLLIENKGQEWYKRNVTALQGFHRHHAEKFDEFLADLPGIVFKVESKEL
                     NLKQLREKYHLDQKTIRKLLRENKGQEWYKRNITALRGYHRRSERSSKFIADLPEIIS
                     QIESGK"
BASE COUNT          537 a          232 c          313 g          428 t
ORIGIN      
        1 gacaggggtt ggattggtat cgacaatatg tggctgttaa ttatgggcgt cccacgagga
       61 acaaagaagc ggctcttctt agatctttac agcatcttac tcgacaaata gaatcaggta
      121 aactgggttt aatttacctg atgagaagat accatttaag tggaaaagct gttaaaaatt
      181 tattaataaa acagaatggc cgggagtggt atgaaactta tattagtgcc aagcgatgcc
      241 atcttaattt aattcaagtg agaacttttt tagctgattt acccgggata gtttcagggc
      301 tagaatcaaa ggaattaaat ttaaccaaat tgacccaaaa atatcatttg gcaaggcaaa
      361 ctattaggaa acttttaaag gaaaataagg gtcaagagtg gtacgatcgg tgcattccta
      421 gctctttagg ttgttgttta gaaaaaaaag ctacttttat ccaatctttg cccgagatta
      481 gccggcagtt agagttggga aaattgaatt tgactaaact agctgataga tacgatttat
      541 caaaacaaac tattaaaaaa attttgaagg aaaaaaatgg tttagattgg tatggcctat
      601 atataactac ccgtcgcggc cgtccacccc taaaggaaaa aactgtgggt gagtcatctt
      661 tgactaaaag aaccatattg attgaatctt tacctcaagt tattcggcag tttgaatcag
      721 gtgaattaaa catgaatcaa ttagtaaaaa attccggttt aaggaaagag attattgtag
      781 aaattttaaa aaaacagaaa ggttccgact ggtataaaac ttatgtaacc gcccgccgcg
      841 gccacggcca ctctcatcag gaaagaaaaa tagcctttag ccgttctttg gatgaaatta
      901 ttcctaagat tgaatcacgt caagtgggag ttgttattct gagtaaacaa tatcatttag
      961 gtgtcgaaac aatcaaacga ttgttggaag aacagaaggg ttctgaatgg tataaagctt
     1021 atgtgactac caaaattggc tggcgtaact cttattggaa aaaagatttt ttgactgatt
     1081 tgcctctgat tacccagcaa atagaagcgg gtcagctaag gttcaggcaa atagccaaac
     1141 aatatcattt agccgaaaag actattagga aattattaat agaaaataaa ggccaggaat
     1201 ggtataagcg taatgttaca gcccttcaag gttttcatcg tcatcatgcg gaaaaatttg
     1261 atgaatttct agctgatttg cctgggatag tttttaaggt agaatcgaaa gaactcaatt
     1321 taaaacagtt gagagagaag tatcatttag atcagaagac tattaggaaa ttattaagag
     1381 aaaataaagg ccaagaatgg tataagcgta atattacagc ccttagaggt tatcatcgtc
     1441 gttcggaaag atccagtaaa tttatagctg acctcccaga gataatttcc caaatagaat
     1501 caggaaaatt
//
LOCUS       DAOOIE010000132         1506 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_68719_length_1506_cov_2.808408, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000132 DAOOIE010000000
VERSION     DAOOIE010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_68719_length_1506_cov_2.808408"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /gene="rplB"
                     /locus_tag="PK131_03130"
     CDS             complement(<1..670)
                     /gene="rplB"
                     /locus_tag="PK131_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPD45145.1"
                     /translation="MKIKQLMTIRKKTGGRDNSGRISVRHHGGEAKRFLREIDWARNM
                     IDITAKVKWIGYDPNRNAPIALINYENGRRGYILAPTNLKVGDVIISSDNAPIKDGNC
                     LCLKNIPVGVPIHGLAYSKDRPAQVVKSAGTSALIQSIENDQAIVKLPSGEIRIFKAD
                     SRATIGQVGNADYINLKLTKAGQKRHRGIRPTVRGVAQHPAAHPHGGGEGRSGVGMNP
                     KTPWG"
     gene            complement(667..954)
                     /gene="rplW"
                     /locus_tag="PK131_03135"
     CDS             complement(667..954)
                     /gene="rplW"
                     /locus_tag="PK131_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPD45146.1"
                     /translation="MILNKPIVTEKALINQQQGRYTFLVASKANKFQIAIAFEQMFGI
                     KPLRINTRRIKGKVKTNWQQRRLIKKTDRKIAVITLPKDKKIDILTIKTKK"
     gene            complement(941..>1506)
                     /gene="rplD"
                     /locus_tag="PK131_03140"
     CDS             complement(941..>1506)
                     /gene="rplD"
                     /locus_tag="PK131_03140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPD45147.1"
                     /translation="LAQGNDKLIAQAVRVFLNNQRCSRAKTKSRGEVVGTTKKMWAQK
                     GTGRARHGTAKAPQFVGGGRAHGPRGNQNFKLKLPQKQRRLAILSVLARLAQDKRIMV
                     IDGLSQLEAKTKMADGLITCLSKTNEVLSKSRRIGIITSRPLKNVKRAFGNLPGIEVL
                     NLHSLNIFNLADKNFLIFTRKALNDFK"
BASE COUNT          419 a          342 c          258 g          487 t
ORIGIN      
        1 taccccaagg agttttagga ttcattccta ctcccgacct accctcacca ccaccgtggg
       61 gatgagcggc cggatgctga gcaactccac gaaccgtggg ccgaatacct cggtgacgtt
      121 tttgtcctgc tttcgttaat tttaaattaa tataatcagc attacctact tgaccaatag
      181 tcgcccggct gtcagcttta aaaatcctga tttctccgct gggtaactta actatcgcct
      241 gatcgttctc aatactttga atcaaagcac tcgttccggc actttttact acctgagccg
      301 gtctatcttt tgaataggcc aaaccgtgaa taggaacgcc aacaggaata ttctttagac
      361 aaagacaatt gccatcttta atgggggcat tgtccgaaga aataatcaca tcacctactt
      421 ttaaattagt tggagctaaa atataacccc gacgaccatt ctcgtagtta atcagggcaa
      481 tgggggcatt tcgattaggg tcataaccaa tccattttac ttttgccgta atatcaatca
      541 tattccttgc ccaatcaatt tctctcaaaa accgtttggc ctcaccccca tggtggcgaa
      601 cacttattcg gccagaatta tcccgaccgc ctgttttttt tctaatagtc attaattgtt
      661 taattttcat tttttagttt ttatagtcaa aatatcaatc tttttatcct taggaagagt
      721 aataaccgct atcttcctgt cggtcttctt aattaaccga cgttgctgcc aattagtttt
      781 taccttaccc ttaattcgtc gggtattaat ccttagcggt ttaataccga acatctgttc
      841 aaaagcaata gcaatttgaa acttattagc cttagaagcc accagaaaag tataccggcc
      901 ttgctgctgg ttaattaaag ctttttcggt aacaataggc ttatttaaaa tcatttaaag
      961 cttttcgagt aaaaattaaa aaatttttat cagccagatt aaaaatatta agggaatgaa
     1021 gattaagcac ttcaattcca ggcaaattac caaaagcccg tttaacattt tttaaaggtc
     1081 tactagtaat tatgccaatt ctacggcttt tcgataatac ttcatttgtc ttagaaagac
     1141 aagtaatcaa gccgtcagcc attttagttt tggcttctaa ttgagataaa ccgtcaataa
     1201 ccatgattct tttatcctgg gctaatctgg ctaaaacaga tagtatcgct aaccgccttt
     1261 gtttttgagg taattttaat ttaaaattct gatttccccg agggccatga gctctgccac
     1321 cgccaacaaa ctgaggggct ttagctgttc catgccgggc tcggccagtg cctttttgag
     1381 cccacatctt tttggttgtt cccacaactt ctcctcgact tttagttttt gcccgggagc
     1441 aacgttgatt atttaaaaaa acacggactg cctgggcaat tagtttatca ttgccctgtg
     1501 ccaaac
//
LOCUS       DAOOIE010000133         1497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_69380_length_1497_cov_2.902913, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000133 DAOOIE010000000
VERSION     DAOOIE010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69380_length_1497_cov_2.902913"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PK131_03145"
     CDS             complement(<1..670)
                     /locus_tag="PK131_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45148.1"
                     /translation="MVQVLKKEWGLIQVPILVGLAIIAVTLPVLNTVVQRNQEMRGKA
                     ATSCGHIGEYCCAGAEGTQPYCLDGLGCDISGGVKCRYQFFACTKDNPYQKGANCKAL
                     YVDQPTKPAVCSLKGVSCWGYEATCQMNCGGCVAEGDPCCTSDNQERFCHNGLECDSG
                     KCKAKVTPGVWYYCDGTQPTCKADSASYSDSAACSRAHGGRWCYYADEANYCNSTCQG
                     AKQLY"
     gene            complement(709..1407)
                     /locus_tag="PK131_03150"
     CDS             complement(709..1407)
                     /locus_tag="PK131_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45149.1"
                     /translation="MRKTKRAGQLPILVGLLVITVALPIASRLVQRDHGIHSWVATAE
                     EGPTPTCDPNKCSDTLGCTMNRCRSTDPVQINCSFCRPISTESDKYVPGCNDNCNICV
                     NPCEPGDTNVTLTAAKADGTSVDFDSFTGLLTIDSSKGFPENKVILKVNITGGTEIKN
                     VGLKFGDGLAEAWAKYYEGSWHGSTTGLGLSFYDSPTVRNEGNNLTVDLPLKVNFEPD
                     REIKIFAQYYGDIF"
BASE COUNT          438 a          398 c          237 g          424 t
ORIGIN      
        1 aataaagttg tttcgcccct tgacaggtac tattacagta atttgcctca tcggcataat
       61 aacaccatct tcccccatgg gcccttgagc aagcggctga atcggaataa ctagcgctat
      121 ctgccttgca agttggctgg gtaccatcac aataatacca gacgcctggt gtcactttcg
      181 ccttacactt tccactgtca cactctaagc cgttgtgaca aaatctctcc tgattgtcgc
      241 tagtacaaca aggatctccc tcagcaacac agcccccaca attcatctga caagtcgctt
      301 cataccccca acaacttact cccttaagcg aacaaactgc tggtttcgtt ggctgatcaa
      361 catataaagc tttacaatta gcccctttct gataaggatt atctttagta caggcaaaaa
      421 actgatagcg gcatttaacc ccgccgctga tatcgcaacc taaaccatcg agacaatacg
      481 gttgggtccc ttctgctccg gcacaacaat attctccaat atgaccgcaa ctagtcgctg
      541 ctttccccct catttcctga tttctctgaa ctactgtatt taacaccggt aaagttactg
      601 ctatgatggc taaacctacc aaaatcggca cctggattaa accccactct tttttcaaaa
      661 cttgcaccat attttattta aacatttacc ttaacttcag tcaaacaatt aaaaaatatc
      721 accataatac tgggcaaaaa tctttatttc cctatctggt tcaaaattaa ctttaagagg
      781 taggtccacc gtcaaattat tcccttcatt tctaaccgtt gggctgtcat aaaaacttaa
      841 gcctaaaccg gtggttgaac catgccaact accttcgtaa tatttagccc aagcttcagc
      901 caaaccatca ccaaacttca gtcccacatt ttttatttcc gtcccaccag taatatttac
      961 cttcaaaatt actttatttt caggaaaacc cttactacta tcgatcgtca acaaaccggt
     1021 aaaactatca aaatcgacac ttgttccatc ggctttagcg gcggtgagag taacattagt
     1081 atcacccggc tcacaaggat tgacacaaat attacaattg tcgttgcaac cagggacata
     1141 cttatctgat tcggttgata tcgggcggca gaacgaacaa tttatctgca ccggatcagt
     1201 cgaccgacag cgattcatag tacagcctaa agtatcactg cacttattag ggtcacaggt
     1261 tggcgttggc ccttcttctg ccgtcgctac ccaggagtga ataccatgat cccgttgaac
     1321 caatctagaa gctatcggca aggccaccgt tataactaat aaaccaacca aaattggcag
     1381 ttgacctgcc cttttagttt ttctcatatt ctttaactat tattaactaa ttttattttc
     1441 acttcaagta ccagtcatac cagcgggaga aatcagctaa ggaagcttga ccatcac
//
LOCUS       DAOOIE010000134         1485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_70218_length_1485_cov_3.158042, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000134 DAOOIE010000000
VERSION     DAOOIE010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70218_length_1485_cov_3.158042"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PK131_03155"
     CDS             <1..465
                     /locus_tag="PK131_03155"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HPD45150.1"
                     /translation="RCRLFKGINHSTIIDSSYNSSPVAAMEMLKFLKTFPHPRVAVLG
                     DMRELGNETENAHRQIYRLARKTADLVIGIGPNTQKFFNPPTFLYWWQAVNFPLPDRA
                     AILVKGSQNTIYLEELVKAWLQDKSDQQFLCRQSPYWQKIKANFKQTIKQSN"
     gene            complement(458..1009)
                     /locus_tag="PK131_03160"
     CDS             complement(458..1009)
                     /locus_tag="PK131_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45151.1"
                     /translation="MRIYFVASPRTVAKNPLVFTKIYRILAKHNKMVDNSILKWTREG
                     FKGSPGYENAVEKVKKAEIVITEVSCHSLSMGFVISQALEFNRPVIALFRKRRKQSFL
                     KWMKNKKLVLAAYDEKTIAKVINRALKEAEKLVDVRFNFLVNQKILAYLDWVARKKMV
                     PRSVFLRDLIEKEMVKDKTYKTS"
     gene            1104..>1485
                     /gene="rplJ"
                     /locus_tag="PK131_03165"
     CDS             1104..>1485
                     /gene="rplJ"
                     /locus_tag="PK131_03165"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPD45152.1"
                     /translation="MPTIEKIEKVLEITQNIKKAGSVVLFQYQGLTASDLAALRAKVK
                     ENGGVIEVIKNSLLSRAISQLGIKLPTELIGPTAVVFCQKDEITPLKEIEKIRQDKEV
                     IEFKYGIYEQKLLTIDQIKTLINLP"
BASE COUNT          488 a          301 c          233 g          463 t
ORIGIN      
        1 cgttgtcgtt tatttaaggg aattaatcac tctactatca tcgatagttc ttataactca
       61 tcgccggtcg cagcgatgga aatgttaaaa tttttgaaaa cttttcccca tccccgagta
      121 gctgttctgg gtgatatgag agaactgggt aatgaaactg aaaatgccca tcggcaaatc
      181 tatcgccttg ctcgaaaaac agctgacttg gttattggta tcggtcctaa tacccaaaaa
      241 ttttttaacc ctccgacctt tttatattgg tggcaggcag tcaattttcc ccttcctgat
      301 cgagcggcta ttttagtcaa aggatcacaa aataccattt atcttgagga attagtgaaa
      361 gcttggcttc aagataaatc tgaccaacaa tttctctgtc gtcaatcacc ttactggcaa
      421 aaaataaaag ctaactttaa acaaaccatc aaacaatcta actagtttta tatgttttat
      481 ctttaaccat ttccttttca atcaaatctc tcaagaaaac tgaccggggg accatttttt
      541 tacgggctac ccaatctaaa taagccaaaa tcttctgatt aactaaaaaa ttaaaccgaa
      601 catcaaccaa tttttctgcc tcttttaagg cccggttaat tactttagca atagtcttct
      661 catcgtaagc ggctagaacc aattttttat ttttcatcca cttcaaaaac gattgctttc
      721 ttctttttct aaaaagagcg attactggtc gattaaactc taaagcctga ctgatcacaa
      781 aacccatcga taaactatgg cacgatacct cagttataac tatttctgct tttttaacct
      841 tttccaccgc attttcataa ccgggagatc ccttaaaacc ttctctggtc cattttaaaa
      901 tcgaattatc aaccatttta ttatgcttgg ctaaaatcct ataaatttta gtaaacacaa
      961 gaggattttt agctactgtt ctaggggaag cgacaaaata tatcctcatc ggggttgtaa
     1021 ttatatcata ccatgatata attccttgtc taaccctaag atagtaggtg ttatcataaa
     1081 tcctactgag ggaaaaggtg tctatgccca ctattgaaaa aatagaaaaa gtcttagaaa
     1141 ttactcaaaa tataaaaaag gctggttcgg tggttttatt tcaataccaa ggtctaacag
     1201 ccagtgatct tgccgccctt agggctaaag tcaaagaaaa tgggggcgta atagaggtta
     1261 ttaaaaattc cctcctcagt cgggccataa gtcaactagg tattaaatta ccaacggagc
     1321 tgatcggccc aacagcagtt gttttctgtc aaaaagatga gataactccc ttaaaggaaa
     1381 ttgaaaaaat tcgccaagat aaggaagtaa tcgaatttaa atatggaatt tacgaacaaa
     1441 aactattgac aattgatcaa attaaaaccc ttattaatct tcctt
//
LOCUS       DAOOIE010000135         1469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_71402_length_1469_cov_2.667610, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000135 DAOOIE010000000
VERSION     DAOOIE010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1469
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71402_length_1469_cov_2.667610"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="PK131_03170"
     CDS             complement(<1..768)
                     /locus_tag="PK131_03170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPD45153.1"
                     /translation="MNDRRKLLGILVLGLFFLVYNFKLSLIWNHDQDLYSWIARDIIV
                     NRHLRLVGQVTSIPGVFIGPFYYYLIALFYWIFRMNPMSAIIPVTAIGLFSIWSLYFV
                     VKEFWGKRAGLISAFLMAVSTGIALFQRWSVPTQPTILWSIWFLYVIFKALEGDKKII
                     YLYGFLLGMVYHIHIALLPILPLPILAYAVSRGSLKEKFSRIKLKEYLGCIAIFLIFS
                     SPFWLFELKHNWSEVKGMMAATQVDIGAPTGKAKFLKV"
     gene            771..>1469
                     /locus_tag="PK131_03175"
     CDS             771..>1469
                     /locus_tag="PK131_03175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD45154.1"
                     /translation="MDYNAVSVVIPCYNEEENLKRGVLDQVDNFLKKQTFQWEVIIAN
                     DHSSDQSLKLIQNFIKNHDKFRVLDLPHGGKPSAVYAGIKAAKYNFALFTDMDQSTPI
                     SELIKLMPYLRDFDLIIGSRGTTRSGNNLLRRLGARIFLTLRRWIILPEINDTQCGFK
                     IIRTDLAKKIFPRLSFFQENIKGGWRVSAFDVEMLFMAQKWGYKIKEVPVLWQNQDIS
                     QTKGNRTNNYFKESI"
BASE COUNT          528 a          281 c          247 g          413 t
ORIGIN      
        1 gactttaaga aattttgcct tgccggttgg agctccaata tctacttgag tagccgccat
       61 cattccctta acctctgacc aattatgttt taattcaaat aaccaaaatg gactgctgaa
      121 aatcaaaaaa atcgcaatac aacccaaata ttcctttaat tttatccgac taaatttttc
      181 ttttaaactg ccccgggaaa ccgcataggc taagatcggc aaaggtaaaa taggcagcag
      241 ggcaatgtga atgtgataaa ccattcctaa taaaaagccg tataaataga taattttttt
      301 atcaccctcg agggctttaa agataacata taaaaaccaa atcgaccaaa gaatcgtcgg
      361 ctgagttggc accgaccaac gttgaaaaag agctattccc gtagaaacgg ccattaaaaa
      421 ggcactaatt aaaccagctc ttttacccca aaactcttta acaacaaaat aaagactcca
      481 aatggaaaat aaaccaatag ccgtaactgg aataatagca ctcatgggat tcatcctaaa
      541 aatccaataa aataaggcaa ttaaataata gtaaaatggc ccgataaaaa cacccggaat
      601 actggtaact tggccgacta accttaaatg gcgattgaca ataatatctc tggcaatcca
      661 ggaataaaga tcttgatcat gattccaaat taaacttaat ttaaagttat aaactagaaa
      721 aaataaccca aggactagaa ttcccagcag ttttctccta tcattcatat atggattata
      781 acgctgtcag tgtggtaatt ccttgttata acgaggaaga aaacttaaaa agaggggttt
      841 tggaccaagt tgataatttt ttaaaaaaac aaacatttca atgggaagtt attattgcta
      901 atgaccattc atccgaccaa agtttaaagc tgattcaaaa ttttattaaa aaccatgata
      961 aatttagagt cttagattta ccccacggcg gcaaacccag cgctgtttat gccggcatta
     1021 aagccgccaa atataatttt gcccttttca ccgacatgga ccaatcaaca cctattagtg
     1081 aattaataaa attaatgccg tacttaaggg attttgatct cattattggt tcacgcggaa
     1141 caacccgaag cggtaataat ctgcttcgcc gattaggggc ccgaattttt ttaactctcc
     1201 gtcggtggat tattttgccc gagatcaatg atacccaatg cggctttaaa attatcagaa
     1261 ctgatttggc taagaaaata tttccacgat tgtctttctt tcaggaaaat attaaaggtg
     1321 gttggcgggt ttcggctttt gatgtggaaa tgctttttat ggctcaaaaa tggggttata
     1381 aaataaaaga agtgccggta ttatggcaaa atcaagatat ttctcaaact aagggcaatc
     1441 gaaccaataa ttatttcaaa gaatcaatc
//
LOCUS       DAOOIE010000136         1462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_71925_length_1462_cov_2.495380, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000136 DAOOIE010000000
VERSION     DAOOIE010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71925_length_1462_cov_2.495380"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1462)
                     /locus_tag="PK131_03180"
     CDS             complement(<1..>1462)
                     /locus_tag="PK131_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45155.1"
                     /translation="WLWKADQLKVGTLYYTTLIQDNDPFIQGQYDDHTYHMQSDWDLL
                     IHTTDSSRTFTLADYRSSNFINTNVGIGTTSPTEKLDVNGKIKTSNFQMTAGAGAGLI
                     LQSDADGNASWAPVLEGLSLTQSGQIYIDYNINPEVLKSLGYEGARDEIETAQFHLNN
                     ASGNLVSRIADFAKIAVAKLTAGFIRADTIKTKTLVNETSIAQDIVTDTIVTKDATVS
                     GTLEARKIIAEDLIVKNEGSINKLMADKVSSLREEIRQMILESGTTTPEATESALVAE
                     SHQWTLETDIASDSAKLTGNLTLTNNLIVGAKLLVSGDAQLGNAFISGTFTAGEIAIK
                     DNFIETTNTALYIQPSGLGSVHIMGDTLIVAENGDVVVSGNLAVNGQLTAHEASVSSN
                     LFAKIIDADEVRTKKLITENISVATDSATTIIAQSGFGELATNSAEIASNATAGTAVL
                     PAGKTELILHNLKLTDKSMVYLTPVGSTNNQVIYLK"
BASE COUNT          367 a          293 c          385 g          417 t
ORIGIN      
        1 ttctttagat aaataacctg attgttggtg gagccgacgg gagtgagata gaccatagat
       61 ttatcggtaa gtttgagatt gtggagaata agctcggttt tgccggcggg cagaacagca
      121 gtaccggcgg tagcattact ggcaatttcg gcggaatttg tcgctagttc accaaatcct
      181 gattgggcga tgatggtagt ggcggagtcg gtagccacag agatgttttc agtgattaat
      241 tttttggttc taacttcgtc ggcatcaata attttggcaa ataggttact ggaaacagat
      301 gcttcatgag cggttaactg accgttaact gccaaattgc cgctgacaac gacatcgccg
      361 ttttcggcca cgattaaagt atcgcccata atgtgaactg aacctaagcc ggaaggttgg
      421 atatagagag cggtattggt ggtttcaata aagttatctt taatagcaat ttcaccggcg
      481 gtgaaagttc cactaatgaa agcattgcca agctgggcat cgccgctgac taatagtttg
      541 gcgccgacga tcaaattatt agttaaagta agattaccgg taagtttggc tgaatcggaa
      601 gcaatatcgg tttccagggt ccactgatga gattcagcta ccaaggccga ttcggtcgct
      661 tcgggggtag ttgtccccga ctccaaaatc atttggcgga tttcctcccg cagtgaactg
      721 actttatcag ccattaattt attgatggaa ccttcatttt tgacgatcaa atcctcggca
      781 ataatttttc tggcctctaa ggtgccgctg acggtggcat ctttggtaac aatagtatcg
      841 gtgacgatgt cttgggcaat gctggtttcg ttaaccaaag tcttggtttt aatagtgtcg
      901 gccctgataa aaccggcggt gagtttggcg acggcgattt tggcaaaatc ggcgatccga
      961 ctgactaagt tgccggaggc attattaaga tggaattggg ccgtttcaat ttcgtcccta
     1021 gccccctcgt agccaagaga cttgagaact tccggattga tgttgtagtc gatatagatt
     1081 tggccgcttt gagttagaga taggccttcg aggacaggcg cccaagaggc attgccatca
     1141 gcatccgact ggagaatcaa accggccccg gccccggcgg tcatttggaa attgctcgtt
     1201 tttattttac cgttgacatc aagtttttcg gtaggtgagg ttgtgccgat gccgacgttg
     1261 gtattgatga aattgcttga tcggtaatca gccaaagtaa aagtgcggct ggaatcagtg
     1321 gtatgaatta gcaagtccca atcgctttgc atatggtaag tatggtcgtc atattgaccc
     1381 tgaatgaacg ggtcattatc ctgaattaaa gtagtgtaat agagggtgcc gactttgagc
     1441 tgatctgctt tccataacca gg
//
LOCUS       DAOOIE010000137         1459 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_72117_length_1459_cov_3.673077, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000137 DAOOIE010000000
VERSION     DAOOIE010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1459
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72117_length_1459_cov_3.673077"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="PK131_03185"
     CDS             complement(<1..1011)
                     /locus_tag="PK131_03185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPD45156.1"
                     /translation="MNGISNKRNKWLIIIILLILILGSSVGWILRDVPGPLKLSKGEF
                     PESSKILDRNGKLLYEIYADKKRTEIPLSEIPQKMIQATLAIEDINFYKHFGFDWRGI
                     IRGAYRTIFQKRLQGGSTLTQQLVKNALLTPERTWSRKVKEALLTLATEIIYSKNQIL
                     EMYFNQIPYGGTLWGVEAAAKDIFNKSAKDLNLAESALLAGLPAAPSKYSPFSHPELA
                     KERQEQVLKRMLEVNFITQEEYQQAIEEKLNYYLVKQNIEAPHFVFYIKEMLTDKYGA
                     ERVTEGGLQVYTTLNLEIQKMAEATVSAEINQLKKLKVTNGATLITSPKTGEILAMVG
                     SKD"
     gene            complement(983..>1459)
                     /locus_tag="PK131_03190"
     CDS             complement(983..>1459)
                     /locus_tag="PK131_03190"
                     /inference="COORDINATES: protein motif:HMM:NF039823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HPD45157.1"
                     /translation="VMALFILFVSIARASLEIASSRDANRDIRKMEIECEVNGEKFTY
                     KLPQARILPDDPIYFFKRVRDWLWLNFAKDKLEKSDIALFIADKKMAETQVLLSKGKI
                     NLALATSQEALNKLKYARSLHPTDQNYYAGWSYAKILQNYNQKIQVLDEWNQQQKK"
BASE COUNT          397 a          272 c          283 g          507 t
ORIGIN      
        1 atcctttgat ccaaccatgg ctagaatttc tcctgttttg ggtgaggtaa tgagagtagc
       61 cccattagta actttcagtt ttttcagttg atttatttca gcactgacgg tggcttcagc
      121 cattttttgg atttctaagt tgagagtagt atagacttgg agtcctcctt cagttactct
      181 ttccgcaccg tatttatccg ttagcatttc cttgatgtaa aagacaaaat gaggagcttc
      241 aatgttttgt ttaactagat agtagtttaa tttttcttca atcgcttgct gatattcttc
      301 ttgggtaatg aaattaacct ctaacattcg ttttaatacc tgctcttgtc tttctttagc
      361 tagttctgga tgagaaaaag gagaatattt agaaggggct gctggtaaac cggccagaag
      421 ggctgattcc gccagattta agtctttagc cgatttatta aaaatatctt tagcggctgc
      481 ctcaactccc cagagagtac caccgtaagg tatttgattg aagtacattt ctagaatttg
      541 gtttttggaa taaataattt cagtggctag ggttaacaaa gcttctttga cctttcgact
      601 ccaggttctt tccggcgtta gtaaggcatt tttaactaat tgttgagtta gggttgaacc
      661 gccctgaagt ctcttttgaa aaatagtgcg ataggcccct cgaataattc cacgccaatc
      721 aaaaccaaaa tgtttataaa aattaatatc ctcaatggct aaagtggctt gaatcatttt
      781 ttgggggatt tccgataagg gaatttccgt tctcttttta tcggcatata tctcatatag
      841 gagtttaccg ttccgatcta atatttttga tgattctgga aattctccct tacttagttt
      901 taaagggccg ggcacatctc gtaagatcca accgacacta ctgccgagga taagaatcag
      961 aagaatgata ataatcaacc atttatttct tttgttgctg attccattca tccaacactt
     1021 ggattttttg gttgtagttt tgcaagattt tagcataaga ccagccagcg tagtaatttt
     1081 gatccgtggg gtgaagggat ctggcgtatt ttaatttatt taaagcctct tgactagttg
     1141 ctagagctag gttaattttt ccctttgata ataaaacctg agtttcggcc atttttttat
     1201 cggcaataaa gagagctatg tcactttttt ctaacttgtc cttagcgaag ttaagccaga
     1261 gccagtccct aactcgttta aaaaaataaa ttggatcatc gggtaagatt cgggcctgag
     1321 gtaatttata ggtaaatttt tctccgttaa cttcacactc aatctccatt tttcttatat
     1381 ctcgattggc atcccgggaa ctggcaattt ctaggctagc ccgggcaata gagacaaaca
     1441 aaataaaaag tgccattac
//
LOCUS       DAOOIE010000138         1449 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_72939_length_1449_cov_2.197991, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000138 DAOOIE010000000
VERSION     DAOOIE010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1449
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72939_length_1449_cov_2.197991"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..976)
                     /locus_tag="PK131_03195"
     CDS             complement(<1..976)
                     /locus_tag="PK131_03195"
                     /inference="COORDINATES: protein motif:HMM:NF021607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2341 domain-containing protein"
                     /protein_id="HPD45158.1"
                     /translation="MARLSLKSLLIIIVVLLIGIVGLWQIQRKAQPVKADWFNESWMY
                     RKSIGISNASGTNQSNFRVNLSIGTSSLIAAGKMKSDCTDVRLTDIQGKLLPHWVQTG
                     TCNTDATVIWTKIPFLSTSANLIYFYYGNPSADDITDSSVLPDSCSTVNAYLGSGLHY
                     VDPDGKTGQNPFQAYCDMTTDSGGWSLVASWGTAPEWTKTSTSTAAVVGGTTPVNTVS
                     ANFGNVVINDFRILASDTATTIASNAYADWYYHYNTATTWKEVWAPTPNKGGDLVHTY
                     RSTSPRQALKPFNYAYNLRFSYQVTQTYNNLSDWGISDQQAGCLPDYWA"
     gene            complement(1010..>1449)
                     /locus_tag="PK131_03200"
     CDS             complement(1010..>1449)
                     /locus_tag="PK131_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45159.1"
                     /translation="ILAGEIGDVQIPTVVYDERSDQTFEFNNIFNGNFPRTALQSKIL
                     NHQKAVSVYSTFLENLEQLKQYHQNGFNLIEMESGPYLRAIKEKFSDLNHLPFDFGIV
                     NYASDNPLSQTLGEGPLNLRGIEPTYLAALAIVQRIIEQELNN"
BASE COUNT          439 a          293 c          324 g          393 t
ORIGIN      
        1 ccgcccaata atcgggcaaa cagccggctt gctgatcact tattccccaa tcgcttaagt
       61 tattgtaagt ttgagtgacc tgataggaaa accttagatt ataagcataa ttaaacggct
      121 tgagagcctg acggggacta gttgaccggt aagtatgaac caaatcaccc cccttattgg
      181 gcgtcggcgc ccaaacttct ttccaagtag tggcagtgtt gtagtggtaa taccaatcag
      241 cataggcatt actagcaatg gtggtggcgg tatcggaagc taaaatccga aaatcgttaa
      301 taaccacatt cccaaaattg gcagagacgg tattaacagg agtagtgccg ccgacgactg
      361 cggcggtgga agttgaagtt ttggtccatt ccggcgccgt tccccaggaa gcaaccagcg
      421 accagccgcc gctgtcggtg gtcatatcgc aataagcctg gaaagggttt tggccggttt
      481 taccgtcagg atcgacataa tgcaagccgc tgccgagata ggcgttgacg gtcgagcagg
      541 aatcaggtaa gacagaagaa tcggtgatat cgtcggcact gggattacca tagtaaaagt
      601 aaattaaatt agcactggtt gacaaaaacg gtatttttgt ccaaataaca gtggcatcgg
      661 tgttgcaagt gccagtttga acccaatgcg gcagtaattt gccctgaatg tcggtaagac
      721 ggacgtcagt gcagtcggat ttcatcttgc cagcggcgat cagagaacta gtaccgatgg
      781 ataaattaac ccgaaaatta gactgatttg ttcccgaagc attgctgatg ccaatgcttt
      841 tccgatacat ccaggattca ttaaaccaat cagctttaac tggctgagct tttcgttgaa
      901 tctgccacaa accaacaatg ccgattaata aaacaacgat gatgattaat aatgacttga
      961 gagacaaacg agccattaaa taattatagt ctaattaaaa ttccaatctt taattattta
     1021 attcttgttc gataattctt tgaacaatgg ctagagcggc taaataggtc ggttcgatgc
     1081 cgcgcaaatt aagcggccct tcacctaaag tttgcgataa aggattgtcg gaagcgtaat
     1141 taactatccc aaaatcaaaa ggtaaatggt ttaaatctga aaatttttcc ttgatagctc
     1201 ggagataagg gccggattcc atttcaatca aattaaagcc gttctgatgg tattgtttta
     1261 attgttccaa attttccaaa aaagtgctgt agacggaaac tgctttttga tgatttaaaa
     1321 ttttactttg aagggcagtt cggggaaaat tcccattaaa aatgttatta aattcgaaag
     1381 tttgatccga tctttcatcg taaacaactg tcggtatttg aacatcacca atttccccgg
     1441 ctaaaatag
//
LOCUS       DAOOIE010000139         1447 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_73119_length_1447_cov_1.555316, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000139 DAOOIE010000000
VERSION     DAOOIE010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73119_length_1447_cov_1.555316"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PK131_03205"
     CDS             complement(<1..278)
                     /locus_tag="PK131_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45160.1"
                     /translation="MIKKISFLVFSLLLGFASLTTSVLAEDQPPMIFDTGKGYLGGCG
                     LTDKTTWTLDEDLEIKLFQIWYNWDANEKEVGITVKKDGEEFLTGTATR"
     gene            complement(298..891)
                     /locus_tag="PK131_03210"
     CDS             complement(298..891)
                     /locus_tag="PK131_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45161.1"
                     /translation="MKTKWPIVIFFLILGSIGFWLVVVKGQKFTPAVSEEDEDETQLL
                     KRINRRETPPLATSTSKNIPTAESAPAVASAVPLPTEEDIIRTFFELIDEKRIPEAIS
                     MMSASMVGDDSSKQAWGVHFNAFDSIMVQKVEPAMEENWTDNSHEYKVTLNVRVSPQA
                     ANAPIPYYGWGNGSNIRWVEIVKENELWKINSLATEP"
     gene            986..1300
                     /locus_tag="PK131_03215"
     CDS             986..1300
                     /locus_tag="PK131_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45162.1"
                     /translation="MRGLIFLIIVFIIFGLPFFMGARSLQKKINRQKESSWKGKLTDK
                     EHVELDNDEGPYSKDVYTLYFETNEGKKIEINTTKDVYDKWKVGDKARKIKGETYPQK
                     IA"
BASE COUNT          474 a          296 c          248 g          429 t
ORIGIN      
        1 cgcgttgctg ttcctgttaa aaactcttct ccgtcttttt taacagtaat tccaacttct
       61 ttttcgttgg catcccagtt ataccaaatt tggaaaagtt taatctccaa atcttcatcc
      121 aaagtccagg tggttttatc agtcaaacca cagcccccta agtaaccctt gcctgtgtca
      181 aaaatcatcg ggggttggtc ttcagccaaa acagaagtag ttaaactggc aaaacccagt
      241 aacaaagaaa aaaccaaaaa gctaatcttt ttgatcattg taaacattat aacaaattta
      301 tggttcagtc gctaaactat ttattttcca aagttcattt tctttgacaa tctctaccca
      361 gcgaatattg gaaccatttc cccatccgta atagggaatt ggggcattag ccgcttgtgg
      421 ggaaaccctc acatttaaag taactttata ttcgtggcta ttatcagtcc aattctcttc
      481 catcgccggt tcaacttttt gaaccataat tgagtcaaaa gcattaaaat gaactcccca
      541 ggcctgctta ctggaatcat cgccaaccat cgaagctgac atcatcgaaa ttgcctcggg
      601 aattctcttt tcgtcaatca gttcaaaaaa agtcctaata atatcttctt ctgtcggcag
      661 aggaaccgct gaagcaactg ccggcgcact ttccgctgta ggaatatttt tagaagttga
      721 ggttgctagt ggcggagtct ccctcctgtt tattcttttc aaaagctggg tttcatcctc
      781 gtcctcctcg gaaaccgccg gggtaaattt ttgtcccttt acgaccacca accaaaaccc
      841 tatactccca agaataagga aaaatataac gattggccat ttagtcttca ttggtttcat
      901 tataaaacaa tttttagcca aaaagtcaag tcaaaattaa aacccttgac aataagaaaa
      961 aaaccgtgtt taatgagaat taaagatgag aggactaatt tttttaatca ttgtttttat
     1021 tatttttgga ctgccgtttt ttatgggtgc cagatcactt caaaagaaaa ttaataggca
     1081 aaaggaatcg tcatggaaag gaaaattgac cgataaagaa catgtggagc tggataatga
     1141 tgaaggtcct tattctaaag acgtttacac tctctatttt gaaactaatg agggtaaaaa
     1201 aatcgaaatt aatacaacca aggatgttta cgataaatgg aaggttggtg acaaagcaag
     1261 aaaaattaaa ggtgaaactt atccccaaaa aattgcttaa aagctaacta tattaaccca
     1321 aacaccggcg aaacagatag tttatctagt ttaaaattga aagatgttag attttgaact
     1381 gttcgccaaa ctgaccaata agcggcaaag gcttttgctc acccttagca gcactgagaa
     1441 tacctat
//
LOCUS       DAOOIE010000140         1441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_73524_length_1441_cov_4.006494, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000140 DAOOIE010000000
VERSION     DAOOIE010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73524_length_1441_cov_4.006494"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1417)
                     /locus_tag="PK131_03220"
     CDS             complement(<1..1417)
                     /locus_tag="PK131_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45163.1"
                     /translation="MSNGAGAPVWINIPGGTTYTASNGITLAGTEFKLGGSLTANTTI
                     AGAYNLNLSQGSLTVGTTTTTASLNLSSTSVGGSSVAAFLSGNSGLVGYLDTSNWDKS
                     TADDYSYWRLTANGTGSTNITNGTTVNFANGVGISLAQAGSTITINNSNPWSDSSNAY
                     IQNQFASNQNASFRISGNGQVGNFFGIGSTSTSPLNVGLTNAAGSSANFLGNITAQKL
                     IDQANSYYFLDPAISDASKNSLSLYMAGSVKFNVADSGTPTHIISGAASRIQNFTNGL
                     LLATDTGTTAAAAVSNWENGLFLSTNGAIGIGFTNPNPLYKLDIGGTLQVSNLAIGGT
                     TVIATPTELNYLSGLAVTNGGVIYTNGTKLANTGVGSSGQYLMSNGAGAPVWTNVGAG
                     GSGATYYAGNGLNLSGTTFNLGGALTQNTRLNIGNTEVLYINYPSGRIGIGTTNPASI
                     SKLTIEGNTHIQGLESDSYALT"
BASE COUNT          409 a          310 c          335 g          387 t
ORIGIN      
        1 cggtgagggc atatgaatca ctctctagcc cttgtatatg agtatttccc tcgatggtaa
       61 gctttgaaat actagcagga ttagttgttc cgatacctat tcgccctgat gggtaattaa
      121 tatacagcac ctcggtattg ccaatattta atctggtatt ctgagttaaa gctccaccaa
      181 gattgaaagt agttccgctt agatttaaac cattgccggc ataataggtt gcccctgatc
      241 ctccggctcc gacattggtc catactggtg ccccagctcc attactcatt aagtattgtc
      301 cggaactacc aaccccggta ttagccagtt tagtgccatt ggtataaatt accccgccat
      361 tagtaaccgc taatcccgac agatagttta attcggtagg agtagcaatg acggtagtac
      421 cgccgatggc gagattgctt acttggagag tcccgccaat gtcaagttta taaagaggat
      481 ttggattggt aaaaccaata ccaatagcgc cgttagtact taagaaaaga ccattttccc
      541 agttggaaac agcagcggca gccgtagtgc cggtgtcggt tgccaacagt aaaccattag
      601 tgaagttttg gattctggaa gcggctccac taataatatg agttggggta ccgctgtcgg
      661 ccacattgaa tttaactgaa ccagccatgt aaagcgatag ggagttttta ctggcatcag
      721 aaatagccgg atcgaggaaa taataagaat tggcctgatc gattaatttt tgagccgtaa
      781 tattaccgag gaaattagct gaggaaccag cggcattagt tagaccaaca ttaagaggag
      841 aggttgaagt cgaaccaatg ccgaaaaagt taccgacttg gccattgccg ctgattctga
      901 agctggcgtt ttggttgctg gcaaactggt tttgaatgta ggcgttgctg gaatcggacc
      961 aaggattgga attattgata gtaatagtgc tgccggcttg ggccaatgaa atgccgacgc
     1021 cattggcaaa attaaccgta gtaccattgg tgatattagt tgaaccagtg ccgttagccg
     1081 ttaaacgcca gtagctataa tcatcggcag ttgatttgtc ccaattacta gtgtccagat
     1141 aaccgactag accactatta ccagagagga aagcggccac tgatgaacca ccgacactgg
     1201 tactactgag attcaatgaa gcagtagtag tagtcgtccc aacagttaat gatccttgag
     1261 ataaattcag attgtaagcc ccagcaatgg tggtgttagc tgttaatgat ccccccaatt
     1321 taaattctgt tccggctaga gtaatgccat tggaagccgt ataggtagta ccaccaggaa
     1381 tattaatcca tactggtgcc ccagctccat tactcattaa gtattgtccg gaactaccaa
     1441 c
//
LOCUS       DAOOIE010000141         1438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_73768_length_1438_cov_3.047722, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000141 DAOOIE010000000
VERSION     DAOOIE010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1438
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73768_length_1438_cov_3.047722"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..170)
                     /locus_tag="PK131_03225"
     CDS             complement(<1..170)
                     /locus_tag="PK131_03225"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPD45164.1"
                     /translation="MAEISTKLGKNLKRIRTTKKLSQGAISRKLEVHRAYISGIENGK
                     RNPTLATIQKLA"
     gene            complement(291..361)
                     /locus_tag="PK131_03230"
     tRNA            complement(291..361)
                     /locus_tag="PK131_03230"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(327..329),aa:Ala,seq:cgc)
     gene            complement(368..871)
                     /gene="pth"
                     /locus_tag="PK131_03235"
     CDS             complement(368..871)
                     /gene="pth"
                     /locus_tag="PK131_03235"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPD45165.1"
                     /translation="MRLFVGLGNPGERYQNTRHNLGVMVINKIVTAPKYYSKLKAKIT
                     KIDGDIFATLDTYMNTSGPAVKKVMKFFKIQPKNLFVIHDDLDLGVGEWKKQFNRSAA
                     GHKGVQSIIDTLGTQAFNRYRIGIGRPADNVSTEDYVLQPFSPAESVIIDQVIKKIIS
                     DFYSVRN"
     gene            958..>1438
                     /locus_tag="PK131_03240"
     CDS             958..>1438
                     /locus_tag="PK131_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45166.1"
                     /translation="MFLPVFSINNRILTNIGTVEAAKAIIDEAPLIPAYEKQFQQEAV
                     IRTVHYGTRIEGNDLSYQEVAKLVDGQKVTAGERDIQEVINYRNVIAYLEELWNLYTT
                     NMPLPEEKTVPNFDDHGRRPFFYSETIVKKIHQLTTFKIIPEEEQGKYRHQQVVLKNT
                     "
BASE COUNT          433 a          262 c          278 g          465 t
ORIGIN      
        1 tcagctagtt tctgaattgt cgcaagcgtc ggatttcttt ttccgttttc aatcccacta
       61 atataagccc ggtgaacttc taatttgcga gaaattgcac cttgcgataa ttttttagtg
      121 gttcttattc gctttaaatt tttgccaagt tttgttgata tttctgccat atagttattc
      181 ccattatatc tcttgttgta tttgtattat aatgatacat agaataaagg gagaaaaatt
      241 gcggcggcga aaaaagaaac ggcgaaagcg aggcaattaa tcaacttctg tggacttact
      301 gggaattgaa cccagaactt acccatgcga agggtacgtt attccgttta actataagcc
      361 cctaatatta atttctaact gaatagaaat cagaaattat ttttttaatt acctggtcta
      421 ttataaccga ttcggcggga gaaaatggct gaaggacata gtcttcggta gacacattat
      481 cggctggtcg gccgataccg atgcggtaac gattgaaagc ctgagtgcca agggtatcaa
      541 tgatcgattg caccccttta tggccggcgg cagagcggtt aaattgtttt ttccactccc
      601 ctaccccgag atctaaatca tcatgaataa caaaaagatt ttttggttga attttgaaaa
      661 atttcattac ttttttgaca gccggaccag aggtgttcat ataagtatca agggtggcaa
      721 aaatatcccc gtcaatttta gtgattttag cctttaattt ggaataatat ttaggagcag
      781 taacaatttt attaataacc attactccta aattatgacg ggtattctgg tatctttcac
      841 ccggattgcc taaaccgaca aagagacgca taaattattg taacaaatcg cccggttatc
      901 taatttccgc atttttccct cattgatttt ttgttttagt ggtataatgc ctttagaatg
      961 tttttaccgg tattctcaat taataatcgt attttaacca atattggaac ggttgaagcg
     1021 gctaaggcga ttattgatga agctcccctg attcctgcct acgagaagca gtttcaacag
     1081 gaggcagtca ttcgcactgt tcattacggc actcgaattg agggtaatga tcttagttat
     1141 caggaggtgg ctaaattagt tgacggccag aaggtaaccg cgggtgaacg cgatattcaa
     1201 gaagttatta attatagaaa tgtaattgct tatttggaag aattatggaa tttatacact
     1261 accaatatgc ccttgccgga agaaaaaaca gtgcctaatt ttgatgacca tggccgccgc
     1321 cccttttttt attcggaaac tattgttaaa aaaattcatc aattaactac ttttaaaatt
     1381 attcccgaag aagaacaggg taaatatcgc catcaacaag tggttttgaa aaatactc
//
LOCUS       DAOOIE010000142         1432 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_74252_length_1432_cov_2.887436, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000142 DAOOIE010000000
VERSION     DAOOIE010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1432
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74252_length_1432_cov_2.887436"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="PK131_03245"
     CDS             complement(<1..841)
                     /locus_tag="PK131_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45167.1"
                     /translation="MNSAVIETHLKNCYAKVFSGREFDLKDLEESFLAMAPILGKNSW
                     DYARRRLPAEIENHPKIYLVSTKNQLNKIDVRVNNWRRVKTESRRRFSYYNQVLNCLA
                     FQISSDADIDDLINNFLALGKNFKIHYLKDDHFDPSQNARFYWDEIFNKSAVINIVER
                     PVYFVSSNLHSLVNLTGGFVYHFQNEIFNFAEKQLPDLAADWEKIKTTNNILRVNDFL
                     YYLSKKYFEANPAKLKEKKDYEYRLGFIQIEPRDYIEAEIQILPLKAVASSQFLDPFL
                     QLTK"
     gene            complement(838..>1432)
                     /locus_tag="PK131_03250"
     CDS             complement(838..>1432)
                     /locus_tag="PK131_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45168.1"
                     /translation="GVLLGVTYLIILLKELVEMRSCAFLHNKILPANKQIKNYLPYLL
                     MPLVLLGIFFYYRGQTGDFWAYFHSGDNFHLNPLPYLVFISTKSWVDTIWLEDIIYIY
                     FLAILTVQKLWRQFKFDILTIYPAVFTMATLFVAHRDISRYLAPVYPFVLYAFKDKLE
                     NKSFRFILWILLPAVFLYAVNFITGNTAPIADWAPYL"
BASE COUNT          505 a          256 c          270 g          401 t
ORIGIN      
        1 tcttggttaa ttgaagaaaa ggatctaaaa attgggatga agccacggct tttaacggca
       61 aaatttggat ttcggcttcg atataatccc gcggttcaat ctggataaaa cccagccggt
      121 attcgtaatc ttttttttcc tttaatttgg ccggattggc ctcaaaatat tttttagaaa
      181 ggtagtaaag aaagtcattc acccggagaa tattattagt tgttttaatt ttttcccaat
      241 ctgccgccaa atcaggcaat tgtttttcgg caaaattaaa gatttcattt tggaaatgat
      301 aaacaaaacc gccggtaaga ttaactaaac tatggagatt agatgaaaca aaatataccg
      361 gcctttcgac aatattaata acagcactct tattaaaaat ctcgtcccaa tagaatcggg
      421 cattttgact gggatcaaaa tggtcatctt ttaaatagtg tattttaaaa tttttaccta
      481 aagccaggaa attattaatt aagtcatcaa tatcagcgtc ggaactaatt tggaaagcaa
      541 ggcaatttaa aacctggtta taataactaa aacggcgccg ggattcagtt ttaacccgcc
      601 gccaattatt tacccgaaca tcaattttat ttaattgatt tttggttgaa accaggtaga
      661 ttttggggtg attttcaatc tcggccggca gacgacggcg agcataatcc caactgtttt
      721 tacctaaaat cggcgccata gccaaaaagg attcttccaa gtcttttaaa tcaaattccc
      781 ggccgctaaa aactttggca taacagtttt ttaaatgggt ttcaataacg gcggaattca
      841 taaatacggc gcccagtcgg caatcggagc ggtattgcca gtaataaaat taactgcgta
      901 caaaaagacg gccggcaaaa gaatccagag gataaatcta aaagacttat tttccagttt
      961 atctttaaag gcatataaaa cgaaagggta aaccggagca agataacggg aaatatcgcg
     1021 gtgagccacg aaaagagtcg ccatggtaaa aacggcagga taaatagtta aaatatcaaa
     1081 tttaaactgc cgccaaagtt tttgaactgt taagattgct aagaagtaaa tataaataat
     1141 gtcttccagc caaatcgtat caacccatga tttggtggaa ataaaaacca aataaggcaa
     1201 agggttaagg tggaaattgt cgccggaatg aaaataagcc caaaaatcgc ctgtttgacc
     1261 acggtaataa aagaaaattc ccagtaaaac taatggcatt aataagtaag gcaaataatt
     1321 cttaatctgc ttgttggcag gcagaatttt attatgtaaa aacgcacaac tgcgcatctc
     1381 tacaagctct tttaacagta taattaggta agttacaccg agaagaaccc cg
//
LOCUS       DAOOIE010000143         1416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_75502_length_1416_cov_3.376929, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000143 DAOOIE010000000
VERSION     DAOOIE010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75502_length_1416_cov_3.376929"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="PK131_03255"
     CDS             complement(<1..192)
                     /locus_tag="PK131_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45169.1"
                     /translation="MTPENESITIILKPKVLKTLAALAAEDETTPENLIGQLILQEDR
                     NRHLVLNKGDEYLVRTKCSR"
     gene            complement(344..796)
                     /locus_tag="PK131_03260"
     CDS             complement(344..796)
                     /locus_tag="PK131_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45170.1"
                     /translation="MGKTEIAKQQGEEYPNQIGGRECLKLIDEIARKVLHGISYELGD
                     YNQFPTISIEEGLEKAKQWYQKTISTPEWCETDYWVKRLEGSIYRAIYATFLAMSKDE
                     NIKMEKIQVDRAYPGSAVTTIDLLVDWSDTVFKKLGHEMSDFYHEDFI"
     gene            1089..>1416
                     /locus_tag="PK131_03265"
     CDS             1089..>1416
                     /locus_tag="PK131_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005429375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HPD45171.1"
                     /translation="MNLSVGIVGLPNVGKSTLFNALLGRQIANIANYPFCTIDPNVGV
                     VEVPDPKLRILADIVKTTKIVPAVVEFVDIAGLVKGASTGEGLGNKFLANIRECQAIC
                     HVVRDFH"
BASE COUNT          344 a          275 c          285 g          512 t
ORIGIN      
        1 ccgagaacat ttcgttctga caagatactc gtcgccttta tttaaaacaa ggtgtcggtt
       61 gcggtcttct tgaagaatta gttggccaat caggttttca ggagtagttt catcttcagc
      121 cgctagagct gccaaagttt ttaaaacctt aggtttaagg ataattgtaa tggattcgtt
      181 ttcaggagtc atcacttaat tcttcctata taacataatt atatcatgtt cggaataaaa
      241 agtcaaggga gctaattttc ctgtgtttac cccaatattc tatttggact ttgtggagcc
      301 agattttggc ctatagtttt atttttggga atcacgggta aatttatatg aaatcctcat
      361 gataaaaatc actcatttca tgcccgagtt ttttaaaaac agtgtctgac caatcaacca
      421 gcaggtcaat agttgttacc gctgatccgg gataggcccg gtccacctga attttttcca
      481 ttttgatatt ttcgtcttta gacatggcca agaaagtagc gtaaatagcg cgatagattg
      541 aaccttctaa tctttttacc caataatccg tttcgcacca ttcaggagtg ctaattgttt
      601 tttgatacca ctgcttcgcc ttttctagtc cttcttcgat tgatatagta gggaattgat
      661 tatagtctcc tagttcgtaa gaaatcccat gaagaacctt tcgggcgatt tcatcaatca
      721 attttagaca ttcccttcca cctatttggt ttggatactc ctccccttgc tgttttgcga
      781 tttctgtttt tcccatcttg ggaaaggata acacattttt tttggtaatc cactaccaat
      841 agtttcttgc ccctttttat tactctctgc ctttcttttt ctagaattaa tttttgccgg
      901 ttaacaccgc ccggatattt cgggttgatt tcgcctcctg ttttcagagt gcgccaattc
      961 gggcaagcga cggcagttta gtgtcttttg accaggccgt gttttttcct taagttgcca
     1021 atttttcgat agctattttt aatagctatg gttttattgt atcaaattgg gtggtagaat
     1081 ttgaggacat gaacctatca gttgggatcg ttggtttgcc taatgtcggc aagtcgactt
     1141 tatttaacgc ccttttgggc cggcagattg ccaatatcgc taattatcct ttttgcacta
     1201 ttgaccctaa tgttggggta gtcgaggttc ccgatcctaa attgagaatt ttagcagaca
     1261 ttgtcaaaac tactaaaatt gtgccggcag tggttgaatt tgttgatatt gccggtttag
     1321 taaaaggggc ctccactggt gaggggttgg gtaataaatt tttggctaat attcgagaat
     1381 gtcaagctat ctgtcatgtg gttcgggatt ttcatg
//
LOCUS       DAOOIE010000144         1401 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_76731_length_1401_cov_3.031204, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000144 DAOOIE010000000
VERSION     DAOOIE010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1401)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1401
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76731_length_1401_cov_3.031204"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="PK131_03270"
     CDS             complement(<1..608)
                     /locus_tag="PK131_03270"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPD45172.1"
                     /translation="MPKNILWFKEISLDDIPIVGGKGANLGEMYRSGIPVPNGFVVTT
                     QAYFHFLETNRLVSLIQNILKITNPDDPNSLLEASKKIKTMIKTSPMDPIITKEIIES
                     YRQLARSYNNKKLAVAVRSSATAEDTADASFAGQQETFLNVIGEDKVVRRVRDCWASL
                     FTPRSIFYRFKKHIDHFKIGVAVPIQKLVQSDVSGIIFTINPV"
     gene            complement(628..1212)
                     /locus_tag="PK131_03275"
     CDS             complement(628..1212)
                     /locus_tag="PK131_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45173.1"
                     /translation="MIFLFHGDDLEKSRRAFNQIIDHFSGETLRIDYHQINLDKINNF
                     LEGASLLSESKMLALSNFFSLSPASQKKIAQIFNQTNTDIAIWQDKKLNPAALKIFPK
                     AKINYFPLPNTLYACLNQIRPHNFKNFAAAYQAALKSVPFDLLFFMIKKTLRQNKYKD
                     AYLQLIELDYQNKTGQLTINKEIALLQIMMTVLT"
BASE COUNT          473 a          205 c          285 g          438 t
ORIGIN      
        1 actggattga tagtaaaaat aatgccggaa acatcagatt gaactaattt ttgaatggga
       61 acagccacac caattttaaa gtggtcaatg tgttttttaa agcggtaaaa gatagatcgg
      121 ggagtaaaaa gtgaagccca acaatcgcgg acacgccgaa ctaccttgtc ttcgccaata
      181 acattaagaa atgtttcctg ctgaccggca aaagaagcgt cggctgtatc ttcagccgta
      241 gccgatgatc tcactgctac cgctaatttc ttattattat atgacctggc taattgacga
      301 tatgattcaa ttatttcttt agtaattatt ggatccattg gactagtttt aatcatcgtt
      361 tttattttct tggaagcttc taatagactg ttcggatcat cggggttggt gatttttaag
      421 atgttttgaa taagtgaaac taatcggtta gtttccaaaa aatgaaaata agcttgggtg
      481 gttaccacaa aaccattggg aacaggaata cctgagcgat acatttctcc taaattagca
      541 cctttaccgc caacgatagg tatatcgtca agagaaattt ctttgaacca gagaatattt
      601 ttgggcatta aaaaatgata acacaaatta agtcaaaaca gtcatcatta tttgaagcaa
      661 ggcaatctct ttgttaatag ttaattgacc ggttttattt tggtaatcca gctctattaa
      721 ttgaaggtaa gcgtctttat acttgttttg acgaagagtt tttttaatca taaagaaaag
      781 aaggtcaaaa ggaacgcttt ttagagcggc ttggtaagcg gcagcaaagt ttttaaagtt
      841 atgagggcgg atttggttaa gacaggcgta gagggtgtta ggcagtggga aatagtttat
      901 tttggcctta ggaaaaattt ttaaagcggc cggattgagt tttttatctt gccagatggc
      961 aatatcggtg ttagtttggt taaatatttg agctattttt ttttgactgg ccggagaaag
     1021 agaaaaaaaa tttgataaag ctagcatttt ggattcgctt aaaagggagg ctccttcaag
     1081 aaagttattg attttatcga ggttaatttg atgataatca atccggagag tttcacctga
     1141 aaaatgatca attatctgat taaaagctcg acggcttttt tctaaatcgt cgccatgaaa
     1201 aagaaaaatc atgcaagagc attataccaa ttagaattcc taaaagactt aaccaagttc
     1261 ccatccagaa aggaagagcg tcggagcgaa agaattcgag aataaatcta ataaaactgt
     1321 aaccgataaa gaaataagca gtttgatgat tttttatttt taatagaatt aaaaataaaa
     1381 aaaaatcgag gattaattca t
//
LOCUS       DAOOIE010000145         1357 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_80691_length_1357_cov_3.022273, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000145 DAOOIE010000000
VERSION     DAOOIE010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80691_length_1357_cov_3.022273"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1357
                     /locus_tag="PK131_03280"
     CDS             <1..>1357
                     /locus_tag="PK131_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45174.1"
                     /translation="QLRDSGVNFSPNVFEVIQALQYLKTNGFKNLDRIKSFNDLITIL
                     SDIGPIRMEIEEEVESEIRKIIAEGKMIDEITRKYLIEKYEGKLKSQVNKEWFKKLVK
                     RYIKQYQAIKRVDEFKENLIKETVKNFPEINKDKLARFTEIKLSNLPKTEKKPIKTDL
                     IAATITKFEPVKNLLTFKTNLIKKAKQEIITKIETQTGIHLSKNEKKEIFQKLDQPAI
                     GEIKNPQIKPILEAEIKKFQEIKNKIQSEIIQLIQPRLDKLNINQEDKNQYQTVIIPK
                     LINDLPLNPTEVKINQKIESAINHSYQDDLQQIIYEELKQNSPLIFQKEFSEKTYQSL
                     ITRTKINQQSDLEQVIQINQLDIFQLLDQQKEELKKQGLTTTAVGSQEVLVFKTLDLK
                     IKTFIKNNPQLINYYRQDRLKQLIQGVIQTETKTITPEQKEIIDNYSEFIAKTYYPTE
                     EN"
BASE COUNT          604 a          211 c          192 g          350 t
ORIGIN      
        1 cagttacggg atagtggggt taattttagt cctaatgttt ttgaagttat ccaagccctg
       61 caatatctta aaactaacgg tttcaaaaat ttggatcgga ttaaaagttt caatgatttg
      121 atcactatct taagcgacat tggtccaatc agaatggaga tagaagaaga agttgaaagc
      181 gaaatcagaa aaataatcgc cgaagggaag atgattgatg aaattacccg aaaatattta
      241 atagaaaaat acgaaggtaa acttaaaagt caggtcaata aagagtggtt taaaaaacta
      301 gtcaagcgct atattaaaca gtatcaagcc ataaaaaggg tagatgaatt taaagaaaac
      361 ttaattaagg aaactgtaaa aaattttccc gaaataaata aagataaatt agcccgattt
      421 accgaaatta aattgtctaa tttgccaaag acagagaaaa aacccattaa aactgattta
      481 attgcggcta ctatcaccaa atttgaacct gtaaaaaatt tattaacatt caagacaaat
      541 ttaataaaaa aagctaaaca ggaaatcatt actaaaattg aaacccaaac gggaattcac
      601 ttgagtaaaa atgagaaaaa ggaaattttt caaaaacttg atcaaccagc gataggagaa
      661 ataaaaaatc ctcagattaa accaatattg gaggcagaga taaaaaaatt tcaggagatt
      721 aaaaataaaa tccaatcaga aataattcaa ctaattcaac cacgattaga taaattaaat
      781 atcaaccaag aggataagaa tcaatatcaa acagtaatta ttccaaagtt aattaatgat
      841 ctacccctaa atccaacaga agttaaaata aatcagaaaa tagaatcggc cattaatcac
      901 tcttatcagg atgacctaca acaaataatt tatgaagagt taaaacaaaa ttcaccttta
      961 attttccaaa aggaatttag tgaaaaaact taccaatctc tcattactcg aaccaaaatt
     1021 aatcaacaat cagacttaga acaagttatc caaatcaatc agttggatat ttttcaactt
     1081 ttagaccaac aaaaagaaga attaaaaaaa caaggattaa caacaaccgc cgttggttct
     1141 caagaagttt tagttttcaa aacccttgat cttaaaataa aaacttttat caaaaacaat
     1201 ccccaattaa ttaattacta ccggcaagac cggttaaaac agttaattca gggggtgatc
     1261 caaaccgaga caaaaacaat tacccccgaa caaaaagaaa taattgataa ttattcggaa
     1321 tttatcgcta aaacttatta tccgacggaa gaaaatg
//
LOCUS       DAOOIE010000146         1340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_82315_length_1340_cov_2.604669, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000146 DAOOIE010000000
VERSION     DAOOIE010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82315_length_1340_cov_2.604669"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1340)
                     /locus_tag="PK131_03285"
     CDS             complement(<1..>1340)
                     /locus_tag="PK131_03285"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HPD45175.1"
                     /translation="EMSLFAKPKAVLWVKRKSVNLYFDRKENNVFNWDLNLFEPHSVE
                     DLAPLTAFVNQQGLKEGVVLVDDDVIITKSFIYDSVIKTIEKKEVISLAESFIPFKID
                     PQYLNYELFPGTDKTLIRASIGNKIKMEVLKANLTQLAFNPLSFETVSTAVSRVISGF
                     FAGEYFLIYPIAKNEYTLFLADKEKVYLTAGLKGTSLDIQKIINYAGLYFSHPISKIF
                     VPSQFELELNATSVLDKTPYDEVQIAREWRKAGNIPLPVLGLFGSLPRVIIRPAMENR
                     KNYLPIIAVFIVTAAIASIVIWFILNRNRTPEVSSPVGPTAVKEQREEVELTTTPTPS
                     VTEIAKDIKIQVLNATDINGQAAAVKEMLTKLGFTSVAIGNSKENVKGNIIRLKEKIA
                     TASSYFKAKLPDFQAEFEELPATSSYDAVFLIGVDLKTGAIVTPQSTATPTAKA"
BASE COUNT          426 a          252 c          239 g          423 t
ORIGIN      
        1 agctttagcc gtcggagtag ctgtcgattg aggagttact atcgctcctg tttttaaatc
       61 aacccctata agaaaaacag catcgtaaga tgaggttgct ggtaactctt caaattcagc
      121 ctgaaaatcg ggtaatttag ctttaaaata agaggaagca gtggcgattt tttccttaag
      181 tctaataata ttgcccttga cattttcctt actgttgccg atagctaccg aagtaaaacc
      241 caatttagtt aacatctctt ttaccgccgc cgcttgaccg ttgatatcgg tggcatttaa
      301 aacctggatt ttaatatctt tagctatttc tgttactgat ggtgtcggtg tcgttgttag
      361 ctctacttct tctctctgtt ctttaactgc tgttggtcct actggtgaac ttacctctgg
      421 agttcgatta cggttgagaa taaaccaaat aacaatcgag gcaattgccg ccgttacaat
      481 aaaaacggcg ataataggaa gataattttt tcgattttcc atagctggtc taattataac
      541 acggggtaga gaaccaaata aacccagaac tggtaaggga atattgccgg ctttcctcca
      601 ttcccgagca atttgaactt catcataagg agttttgtct aaaaccgaag tagcattaag
      661 ttccagttca aactggcttg gcacgaaaat tttagaaata ggatgggaaa aatataagcc
      721 agcataatta ataatttttt ggatatctaa acttgtccct tttaatccgg cggttaaata
      781 aactttctcc ttgtctgcca aaaagagggt atattcattt ttagctatag ggtaaattaa
      841 aaaatattcg ccagcaaaaa atccagagat tactcgagaa acagctgtag aaaccgtttc
      901 aaatgataag ggattgaagg ctaattgagt caaattagct ttaagaactt ccattttgat
      961 cttgttacca atagaagctc gaataagagt cttatctgtt cctggaaata attcataatt
     1021 aagatattga ggatcaattt taaaaggaat aaaactttcg gctagactaa tgacttcttt
     1081 cttctcgatg gttttaataa ccgaatcgta aataaacgat ttggtaataa tcacatcatc
     1141 atcaactaaa accactcctt cttttaaacc ctgctggtta acaaaagcag ttaagggagc
     1201 taaatcttca accgagtggg gctcaaatag atttaaatcc cagttaaaaa cattattttc
     1261 cttccggtcg aaataaagat taacgctttt tctcttaacc caaagaaccg ctttaggctt
     1321 agcaaataat gacatctctc
//
LOCUS       DAOOIE010000147         1337 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_82577_length_1337_cov_3.252730, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000147 DAOOIE010000000
VERSION     DAOOIE010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1337
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82577_length_1337_cov_3.252730"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1337)
                     /locus_tag="PK131_03290"
     CDS             complement(<1..>1337)
                     /locus_tag="PK131_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45176.1"
                     /translation="LVESVENDESFSNSFILINLGRRQFNLSLVYFGKVKERFHETFS
                     DGFSPKAIENVLANLSSQSTLPPLLIFFGDTPNLPIDDIKNYPWIGKKIGDIFFHLPD
                     LKFLSLEEITRIWASFINKQIIGPVESGSLGPTEEAVSPAEVEPAAVESLPPVESETA
                     LSMEPPAEVEPPTAVVPETTSPPVEVPMAQLDLKPKIRPKLPKIKLRLKLKLNAFLWF
                     LSFIPLLFLGLWLLNRVEIVLLLKPLPLETTIPVTLDSQTATFSPGSRVIPAVIQSFN
                     LKVSDKVPTTGKKTVGEKAKGSVVIFNKEEKVLNLPVGTILMTADNKQFELLTPVQVA
                     GGNLDFNQEPAVLKLGQTKTVVGAVDIGPEFNLSQGAKLVFKDYPESVLIARTEADFT
                     GGSRQEVQVVDRQDKIELESRLKRAVETTIKEKIEKDINNLAGALPETVRTQS"
BASE COUNT          395 a          276 c          254 g          412 t
ORIGIN      
        1 tggattgagt tctaacagtc tctggcaagg ccccggccaa attattaata tctttttcaa
       61 ttttttcttt aatagttgtt tcgactgctc tctttaggcg cgattctagt tctatcttat
      121 cttgacggtc aacaacttgg acttcctgcc gactaccacc ggtaaaatct gcttcggttc
      181 tggctattaa aacactctcg ggataatctt taaaaaccaa ttttgctccc tgggaaagat
      241 taaactccgg cccaatatcg accgccccaa ccactgtttt agtctgaccc agtttaagta
      301 ctgccggctc ttgattaaaa tctaaattgc cgccggcaac ttgaaccgga gttaataact
      361 caaactgttt attgtcagca gtcatcaaaa ttgttccaac tggcaaattt agcacttttt
      421 cctccttatt aaaaataaca acactcccct tggctttttc cccaacagtt tttttaccgg
      481 ttgtaggaac cttgtcgcta actttgagat taaaagattg aattactgcc ggaattaccc
      541 tcgatccggg actgaaagtg gctgtttggg aatctaaagt aactggtata gttgtttcca
      601 aaggcagggg ttttaataaa aggacaattt ctacccgatt taacaaccat aagccaagga
      661 ataatagagg gataaaagat aaaaaccaaa gaaaagcatt tagttttaat tttaatctca
      721 atttaatttt gggtaatttg ggccttattt taggttttaa gtctagttga gccatcggca
      781 cttcgactgg aggggaggtt gtctcgggaa ctactgccgt cggtggttct acttcggccg
      841 gcggttccat tgataaggct gtttcagact cgactggcgg caatgattcg actgctgctg
      901 gttcaacttc agctggagag actgcctctt ctgtcggtcc taaagatccc gactctaccg
      961 gaccaataat ctgtttatta ataaaactag cccaaattcg ggtaatttcc tccaaactta
     1021 aaaactttaa atcgggtaaa tgaaagaaaa tatcaccaat ctttttacct atccaagggt
     1081 aattcttgat atcatctatc ggcagattgg gagtatcacc aaaaaaaatc aatagaggtg
     1141 gtagggtcga ttgggacgag agattagcta aaacattttc aatcgctttg ggagaaaaac
     1201 cgtcgctaaa agtttcatga aatcgttctt ttactttgcc aaaataaacc aaagaaagat
     1261 taaattgacg tcggcctaga ttaattaaaa taaaactatt actaaatgac tcgtcatttt
     1321 caactgattc gactagg
//
LOCUS       DAOOIE010000148         1336 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_82710_length_1336_cov_2.170180, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000148 DAOOIE010000000
VERSION     DAOOIE010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82710_length_1336_cov_2.170180"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="PK131_03295"
     CDS             <1..741
                     /locus_tag="PK131_03295"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HPD45177.1"
                     /translation="NKDHFPILDSPSYILVDAETNTVLAQKNSLIRVFPASTTKLVTA
                     LTALNIYPLEEIVTISRVYPEGKTMGLVPGEKMTVLSLVQALLVSSANDAAYNLADHH
                     LNGTAGFVTDMNRLVVRYGLKNTHFVNFDGIHHPNHYSTAFDLAQLARLAIKNPIITE
                     VASQKEITVTDVDRFHQYHLTTTNELLGVIPEIKGLKTGWTPEAGGCFISLIDLNGHQ
                     LIGVVTQSQDRFKDTKKLIDWAKNSVIW"
     gene            complement(731..>1336)
                     /locus_tag="PK131_03300"
     CDS             complement(731..>1336)
                     /locus_tag="PK131_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45178.1"
                     /translation="DGSFHLTYPYNEGQMLMGNVNLPSKEELITKAKSFLAEAKKLPQ
                     DIGENKVSYWKNEGSGLKQVVSQSEANMARIDFYRTNVYDNFKIVSTDVNRAPISVLL
                     TGLSVSGKDIVEVDYKYVNIDRDSFATYPIKSTAEAYNDLKSGNYWPAADVTASTVTI
                     RKINLAYFEPVALTNFMEPVYVFEGDNFTAYVPAVANQFTK"
BASE COUNT          411 a          254 c          252 g          419 t
ORIGIN      
        1 aataaggatc attttcccat tctcgattct ccgtcctata ttttggtcga tgccgaaact
       61 aatactgttt tagcccaaaa gaattcttta atccgggttt ttccggcttc aacaactaaa
      121 ttagtaaccg ctctaaccgc cttgaatatt tatcctctgg aagaaattgt tactatttct
      181 agagtttacc cagaggggaa aacaatgggt ttagtgccag gagaaaaaat gactgtactt
      241 tccctagtcc aagctttatt agtttcttcg gccaatgatg ccgcttataa cttagctgac
      301 catcatctga atggaacagc cggttttgtg acagatatga atcggttagt agttagatat
      361 ggtttgaaga atactcactt tgttaatttt gatggaattc atcatcctaa tcactattcg
      421 acggcttttg atctggctca attggctcga ctagcaatta aaaacccgat cattactgag
      481 gtagcttccc aaaaagaaat aacagtgact gatgtcgatc ggttccatca ataccattta
      541 acaactacta atgaactttt aggagttatt cccgaaatta agggtttaaa gactggttgg
      601 acaccggaag ctggaggttg ttttattagt ttaatcgatc ttaatggtca tcaattaatt
      661 ggggtagtta cccaaagtca ggacaggttt aaggatacaa aaaagttaat agattgggct
      721 aaaaactcag ttatttggta aattgattgg ctacagctgg tacataggca gtaaaattat
      781 cgccttcaaa aacatagact ggttccataa aattagttag ggcaaccggt tcgaaataag
      841 ctaaattaat ttttctgata gttactgtcg aagcagttac atcggctgcc ggccaataat
      901 taccgctttt cagatcatta taagcctcag ccgttgattt aatagggtag gtagcgaatg
      961 aatcacggtc aatattgacg tatttatagt cgacctcaac gatatcttta ccactcacac
     1021 taaggccggt taacaaaacc gagataggag cacgattaac atcggtagag acaattttaa
     1081 aattgtcata aacattggtt cgataaaaat caatccgggc catattagcc tccgattgac
     1141 tgactacctg tttcaaaccg gaaccctcat ttttccagta tgaaacttta ttttcaccga
     1201 tatcttgggg taatttttta gcttcagcta ggaaagattt cgccttggta attagttcct
     1261 ccttactggg taaattaaca ttacccataa gcatttggcc ttcattatag ggataagtta
     1321 aatgaaaact accgtc
//
LOCUS       DAOOIE010000149         1319 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_84385_length_1319_cov_2.837025, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000149 DAOOIE010000000
VERSION     DAOOIE010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1319)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84385_length_1319_cov_2.837025"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PK131_03305"
     CDS             complement(<1..506)
                     /locus_tag="PK131_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45179.1"
                     /translation="MKHKKYLFLIIFNLIAILYLIQPVPNLPDLPQSVKSSLPGDTIQ
                     ISHVRAYFTNLSRAEVIDFYRTHYRGLGRIIINHPPEKAKEIFVDTIQSYYLEEFILP
                     FKESLYINGFEWENDVFTKPEKRIANKLIYENQTFKSKVTIRTFSTSISQRLMMLLLT
                     EVILFFIC"
     gene            complement(503..1060)
                     /locus_tag="PK131_03310"
     CDS             complement(503..1060)
                     /locus_tag="PK131_03310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPD45180.1"
                     /translation="MMPKILNRLQTIILEFWLLILRIVGHIPSHMIRKFFYLLSGVKM
                     NYFKSTIHIGANFFKPSGITIGQDTIIGDHCFLDGRAPLKIGNHVGIASQVLIYNDEH
                     DINSPTYGNSFGPVEIGDYVFIGPRVIILPGVKIGRGAVIAAGAVVTKNIPDFEIWGG
                     VPARKIADRQIKNPSYKLGRAMLFQ"
     gene            complement(1041..>1319)
                     /locus_tag="PK131_03315"
     CDS             complement(1041..>1319)
                     /locus_tag="PK131_03315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPD45181.1"
                     /translation="LYEGFGIPAIEAQKGGLPVIASRIPIYEEILGKSALLINPLNSD
                     ELVNAFKTVQNDAVAQKLIKAGFKNAAKFTWAKTAHSILKCFYDAKNS"
BASE COUNT          439 a          229 c          266 g          385 t
ORIGIN      
        1 ttacagataa aaaagagaat cacttcggta agcaaaagca tcatcaaacg ctgagagatt
       61 gaagtggaaa aagttcggat agttaccttt gatttaaaag tttggttttc gtagattagt
      121 ttgttggcaa ttcttttttc cggtttagta aaaacatcat tttcccattc aaaaccatta
      181 atataaagag attctttaaa aggaagaata aattcctcaa gataatagct ttgaatagtg
      241 tcaacaaaga tttctttagc cttttctggc gggtgattaa taataattcg acccaatccc
      301 cgataatgag tccgatagaa atcaatgact tcagctcgag aaaggttagt gaaataggcc
      361 ctaacgtgac taatttgaat agtatcaccc ggaagagatg atttaaccga ttggggaagg
      421 tcaggaagat tgggaacagg ttgaattaga taaaggatgg cgattagatt gaaaataatc
      481 aggaaaagat attttttatg tttcattgaa aaagcattgc tcgacccagt ttgtaactgg
      541 gatttttaat ttgacgatcg gctatttttc gagcggggac cccgccccaa atttcaaaat
      601 caggaatatt tttagtaacc accgctccgg cggcgattac tgctccccgc ccaattttaa
      661 caccaggaag gataattact cgcggcccga taaaaacata gtcgccaatt tccactggtc
      721 caaaagaatt accgtaggta gggctattaa tatcatgttc atcgttatag attaatacct
      781 gagaggcgat accgacatga ttgccaattt taaggggcgc tcgaccgtcg aggaaacagt
      841 gatcaccgat aatagtatct tgaccgatgg ttatccctga tggtttgaag aaattggccc
      901 ctatatggat ggttgatttg aagtaattca ttttaacgcc tgaaagaagg taaaaaaact
      961 tacgaatcat atgggaagga atatgaccga caattcttaa aattaaaagc caaaactcca
     1021 aaataatggt ttgaaggcga ttaagaattt ttggcatcat agaagcattt taatatagaa
     1081 tgagcggttt ttgcccaagt gaatttagcc gcgtttttaa atccagcttt aataagtttt
     1141 tgggctaccg catcattttg aacggtttta aaagcgttaa ccaattcatc cgaatttaag
     1201 ggattaatta acaaggctga tttgcctaaa atttcctcat aaataggaat tcgactggca
     1261 atgaccggca gccctccttt ttgagcttca atagcaggaa taccaaaacc ttcataaag
//
LOCUS       DAOOIE010000150         1315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_84747_length_1315_cov_4.037302, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000150 DAOOIE010000000
VERSION     DAOOIE010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84747_length_1315_cov_4.037302"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PK131_03320"
     CDS             <1..186
                     /locus_tag="PK131_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HPD45182.1"
                     /translation="AIYEVMPVSEKIAKLIVSKAAAADIQKQAIEEGMLTMKQDGYVK
                     VLEGITTIEEVLRVAQY"
     gene            233..1294
                     /locus_tag="PK131_03325"
     CDS             233..1294
                     /locus_tag="PK131_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HPD45183.1"
                     /translation="MNAKLQEILKLAISSEASDVHLIEGMVPKLRVRGQLIEVNNFPD
                     DSALIAEMILSTLSPDQETRFKQEKELDYSLEMADHRFRANAYFQQGRMAISLRVIPA
                     AIPTFEELKLPEIFKSFVEVKQGFILVTGPTGTGKSTTVASILNEINKRRNCHIVTIE
                     DPIEYIIKPENSIISQREIGFDTNDFARALRSSLRQDPNVVFVGEMRDLETISTALTV
                     AETGHLVFSTLHTNSAAQTVDRIVDVFDQKEQVRVQLAAVISAIISQRLIPTLEGQLI
                     PAFEILIATPAVKNCIREGKAFMLDNIIQTGADIGMVSLDYSLASLVKQGKVTEETAM
                     SFSLKPSALQNYLREIKII"
BASE COUNT          379 a          227 c          302 g          407 t
ORIGIN      
        1 gctatttatg aagttatgcc ggtttcggaa aaaatcgcta aattaattgt ttccaaggcg
       61 gcggccgctg acatccagaa gcaggccatt gaagagggca tgttgacaat gaagcaggat
      121 ggctatgtca aggtcttgga gggtattacc accatcgagg aggtgctgcg ggtggcgcaa
      181 tattagttag taactagtaa ctagtaacta gtaattagta attagtaaaa aaatgaacgc
      241 aaagttgcag gaaattttaa aattagctat ttccagcgag gcttcggatg ttcatctaat
      301 tgagggtatg gtgccgaagt tgcgggttag gggccagtta atcgaagtta ataattttcc
      361 tgatgattcg gctttaatag ctgagatgat tttatcgact ttatcacctg atcaggaaac
      421 aagatttaaa caagaaaaag agctggatta ttcgttggaa atggcagatc accgttttcg
      481 ggctaatgct tattttcagc aaggccggat ggcaatttca cttcgggtta ttcccgccgc
      541 cattccgact tttgaggaat taaaattacc ggaaattttt aaatcatttg ttgaagttaa
      601 acagggtttt attttagtta ccggtccaac cggaacaggt aaatcaacca ctgttgcctc
      661 aattttgaat gaaattaaca aacggcgtaa ctgccatatt gttactattg aagatccgat
      721 tgaatatatt attaaaccgg agaattcgat tatctctcaa cgggaaatcg gttttgatac
      781 taatgatttt gcccgggctc ttcgttcctc gcttcgtcag gatccaaatg tcgtttttgt
      841 tggtgaaatg cgggatttag aaacaatttc taccgctttg acggtggcgg agactggtca
      901 tttggttttt tcgactttac atactaactc ggccgcccaa acagttgata gaattgttga
      961 tgttttcgat caaaaggaac aagttcgggt tcagttggcg gcagtgatta gcgcgataat
     1021 ttcccaacgg ttgatcccga ctttagaagg tcaattaatt ccggcttttg aaattttaat
     1081 tgccactccg gccgttaaaa actgcatcag ggaaggcaag gcttttatgc tcgataatat
     1141 tattcagact ggagccgata taggtatggt cagcctagat tattccttgg ctagtttggt
     1201 gaagcagggt aaggtaactg aagaaacggc catgagtttt agtttaaaac ctagtgccct
     1261 ccaaaattat ttacgggaaa ttaaaattat ttagttaata tgcctaaatt tttat
//
LOCUS       DAOOIE010000151         1312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_85068_length_1312_cov_3.116150, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000151 DAOOIE010000000
VERSION     DAOOIE010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85068_length_1312_cov_3.116150"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="PK131_03330"
     CDS             complement(<1..337)
                     /locus_tag="PK131_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45184.1"
                     /translation="MAEFKRSRLARKTDEQITRKTVFLGAVTIILFLILVVFGLPLLV
                     RFSIFLGENRRTKDVIQEKVLPPQPPRVILPYEATNTAKIDITGVAEPKVKVILLKDD
                     VNLEESQVSN"
     gene            complement(409..481)
                     /locus_tag="PK131_03335"
     tRNA            complement(409..481)
                     /locus_tag="PK131_03335"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(446..448),aa:Thr,seq:cgt)
     gene            complement(502..>1312)
                     /locus_tag="PK131_03340"
     CDS             complement(502..>1312)
                     /locus_tag="PK131_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45185.1"
                     /translation="KTAELIERISAIRKALLEFGATELEIDLSNRLPDNDQIHLLSPV
                     QFQYETEMREGIGGVSNIPGEIIIKNRLYVGNILYAISHELVHTVSYKSIHATELKMD
                     FVRWGYSTHQTDSKTVFDLVDEAVTEMTNYEIASNYWPKQDLLNTDNILSKVEYHFRP
                     SVILFDEIIKKVASVKGWKYKTVWRELQKGNFLGESKGLKMITQVFGKERMKKLADWR
                     ENSAKSAVVMAMNLANDKNELEWAEKIGVKILKGEYQINNIPIMGDIIKQV"
BASE COUNT          384 a          305 c          198 g          425 t
ORIGIN      
        1 cattgctcac ctggctctct tctaaattaa catcgtcttt caataagata accttcactt
       61 tcggctcagc cactccagta atatcaattt tagcggtgtt agttgcctcg tagggtaaaa
      121 tgactcgcgg cggttgaggc ggtaaaactt tctcctgaat cacatcttta gttcgacgat
      181 tttctcccag aaaaatagaa aatctgacta aaagtggtaa accaaaaact accaaaatca
      241 aaaataaaat aatagttact gctcccaaaa aaacggtttt gcgggtaatc tgctcgtcgg
      301 ttttgcgggc taatcttgat cgtttaaact ccgccatagc tagctatatt ctaccaaaaa
      361 catgatctat aatctacgat ccgccgattg gcgggcaggt ctagtttgag ccagagggga
      421 ggattgaact cccgacctaa gctttacgaa agcctcgctc tcccgactga gctactctgg
      481 caacaagtta ttttaacact cctaaacctg cttgataata tcccccatta tcgggatatt
      541 atttatttga tattcacctt tcaatatttt tacaccaatt ttttccgccc actctaattc
      601 atttttatca ttggctaaat tcatggccat tacaacagca ctcttggcag aattctccct
      661 ccaatctgcc aatttcttca tcctttcttt cccaaatact tgagtaatca ttttcaatcc
      721 tttggattcc cctaaaaaat ttcccttttg gagttctctc cataccgttt tatatttcca
      781 cccttttact gaagcaactt tcttaataat ctcatcaaat aagatcacag aaggacgaaa
      841 atgatattcc actttactta aaatattgtc tgtatttaat aaatcttgct tcggccaata
      901 attagatgcg atctcataat ttgtcatctc cgttaccgct tcgtccacta aatcaaagac
      961 agttttggag tcggtttgat gagtactata accccaacga acaaaatcca tttttaattc
     1021 cgtagcatgg atggatttat aagagacggt atgaaccaat tcatgactaa tagcataaag
     1081 tatattgcca acatataggc gattcttaat gattatttcc ccaggaatgt tagaaactcc
     1141 gcctatccct tccctcattt cggtttcata ctgaaactga actggactca aaagatggat
     1201 ctgatcatta tcgggcaaac gattggacaa atctatctct aattcagttg caccaaattc
     1261 caataatgct tttcttatgg ccgatatcct ctctattaac tcggcagtct tt
//
LOCUS       DAOOIE010000152         1284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_87984_length_1284_cov_2.733930, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000152 DAOOIE010000000
VERSION     DAOOIE010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_87984_length_1284_cov_2.733930"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1284)
                     /locus_tag="PK131_03345"
     CDS             complement(<1..>1284)
                     /locus_tag="PK131_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45186.1"
                     /translation="NGITLAGTEFKLGGSLTANTTIAGAYNLNLSQGSLNVGTTIKSA
                     NLYTTGSVGIGNSSPVQALDVTGAIRASIGVTAPKITLTTGPTNGYILVSDASGNGTW
                     TNPAGIGGTNYFAGNGITLTPTNVFELGSSLTRNTTIGGAFTLNFLNTGVTMAALSVN
                     STLSTTSLAIGNTVLVTNAARLNYLQGIANTAGSLVYGNGTNLANTSVGSSGQILMSN
                     ASAAPTWVNANSIGIGTTYAANNGLTLTGANIFQLGGLLMQNTDIAFSGFNLSFSDAG
                     TTLFNINSAGANFNVPTAFNSSGDISLAYDLNFTNNTSSNIITVAPLTIEVGENFESN
                     NLTLKTYNAGKIIFDSSNLYTDGTYFGIGTTNPTTTLDVTGAINTSIGITAPKITLTT
                     GPTNGYILVSDASGNGTWTNPAGIGGTNYFAGNGI"
BASE COUNT          394 a          251 c          316 g          323 t
ORIGIN      
        1 taatcccgtt accggcaaag taattggtac cgccgatccc agccggatta gtccaagtac
       61 cattcccaga ggcatcagaa actaaaatat aaccattggt tggaccggta gtgagagtaa
      121 ttttaggagc agtaatcccg attgaggtat tgatggctcc ggtaacatca agagtagtgg
      181 tagggttagt agtcccaata ccaaaatagg tgccatcggt ataaaggtta ctggagtcaa
      241 aaataatttt accggcatta tatgttttga gagtgagatt gttgctttca aaattttccc
      301 caacttcgat agtcagagga gcgacagtga tgatgttact ggaagtgttg ttggtgaagt
      361 tgagatcata agcaagggaa atgtcgccgc tggaattaaa agccgtcggc acattgaaat
      421 tagccccagc ggaattgatg ttgaacaaag tggtcccggc atcggagaag cttaagttaa
      481 aaccagagaa agcgatatcg gtgttttgca tcagtaaacc accaagctgg aaaatattgg
      541 caccggttaa ggtaagacca ttgttggcag cataagtggt gccaatacca atgctattgg
      601 catttaccca agtgggggcg gcactggcat tgctcattaa aatttggccg ctggaaccaa
      661 cactggtgtt agctaaatta gtgccattgc cgtagaccag agatccagcc gtgttggcaa
      721 taccctgaag ataatttaat ctggcggcat tagttaccag gacagtgttg ccaatggcca
      781 aagaagtggt tgaaagagtt gaattaacag aaagagcagc catagtaacc ccggtattga
      841 ggaaattaag agtgaaagcc ccaccaatgg tggtgttacg agttaaagaa gagcctaatt
      901 caaaaacatt agtaggagtt aaagtaatcc cattaccggc aaagtaattt gttccgccga
      961 tcccagccgg attagtccaa gtgccattcc cagaggcatc agaaaccaga atgtagccat
     1021 tagttggacc ggtagtgaga gtaatcttgg gagcggttac tccaatagat gccctgatgg
     1081 ctccagtaac atccaaagct tgaacgggag aagaattacc gatccctaca ctaccggtag
     1141 tgtataaatt agctgattta atagtggtgc caacatttaa tgatccctga gataggttca
     1201 aattgtaagc cccagcaata gtggtgttag ctgttaatga tccccctaac ttaaattctg
     1261 ttccggctag agtaatgcca ttag
//
LOCUS       DAOOIE010000153         1273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_89250_length_1273_cov_1.966338, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000153 DAOOIE010000000
VERSION     DAOOIE010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1273
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89250_length_1273_cov_1.966338"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1273)
                     /locus_tag="PK131_03350"
     CDS             complement(<1..>1273)
                     /locus_tag="PK131_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPD45187.1"
                     /translation="VVGLAIPLVFFATFGVMLMTDISLNFLSLFSLILALGLLVDDAI
                     VVVSATKQYLRSGKFTPEEAVLLVLNDFKMVLTTTTLTTVWAFLPLLLATGIIGEFIK
                     SIPITMSVTLVTSLIIALIINHPLAAILERIRFTRKLFSFLFVGLIVISLGVWFLPLS
                     IVWKLALSLPSLAAVIFLGRWYRHRGQKVLENNSVLVEQEWRDDDAIKKKLREQGSHD
                     NNNFLNRLCHGVIHFDRILPYYEKYLRRLLATKRSRLLTLAVTFFLFLVAVSLPATGI
                     IKSEFFPSYNSDVLYINLEAPSGLRLEETDKIVAQVEQRLFAYPEILNFTTLVGSSAS
                     FNGLGGGNNSSHLASITIKLSKTDERKITSYQLADKIRADLSDIKAAKITVESLAGGP
                     PFGAAFEAQIKGDDLQVLDKIANDLEVYLRS"
BASE COUNT          463 a          265 c          241 g          304 t
ORIGIN      
        1 attgaacgca aataaacttc taaatcattg gcaatcttat ccaagacctg caaatcatca
       61 ccttttattt gcgcttcaaa agcggcgccg aaaggcggtc cgcccgctag agattccact
      121 gtaattttag ctgctttaat atcgctcaaa tcagccctaa ttttatctgc caactgataa
      181 gaagtaattt tgcgctcatc agttttactc agcttaatcg taatcgaggc taagtgtgaa
      241 gagttattcc ctccccctag accgttgaaa gaggcggaag atccaactag agtggtaaaa
      301 tttaaaattt ccgggtaagc aaacaaacgc tgttcgactt gcgcgacaat cttatcggtt
      361 tcttctaaac gtaaaccaga aggcgcctct aaattaatat acaaaacatc ggaattgtaa
      421 ctgggaaaaa actcactctt aatgatgccg gtggcaggta aggaaacggc gactaaaaat
      481 aggaaaaatg taaccgccaa agtaagcaaa cgactgcgtt tagttgctaa aagccggcgc
      541 agatattttt cataataagg cagaatgcgg tcaaaatgga taacaccatg acacaagcga
      601 tttaaaaaat tattattatc atgacttccc tgctcacgta attttttctt gatagcatca
      661 tcatctcgcc attcctgttc tactaggaca ctattattct ctaatacttt ttggccgcgg
      721 tggcgatacc agcgtcccaa aaaaataacc gctgccaaag aaggtagaga tagggctaat
      781 ttccaaacaa tagataaagg caaaaaccaa acgcctaaag aaattacaat caaaccgaca
      841 aataaaaagg agaataattt gcgcgtaaaa cggatgcgct ccaaaatagc ggctaaagga
      901 tggttgataa ttaaagcaat aatcaaagaa gtgactaaag taacagacat ggtaatggga
      961 attgatttga taaattcacc gataatgccg gtggcgagca aaagcggcaa aaatgcccag
     1021 accgtagtca aagtggtggt ggttaaaacc atcttaaaat cattcaatac caataaaaca
     1081 gcttcttctg gggtaaactt gccagaacga agatattgct tggtggcgga aacgacaacg
     1141 atagcatcat caaccaataa gcctaaagct aaaatcaacg aaaataaaga caagaaatta
     1201 agactaatgt cggtcatcaa catcacaccg aaagtcgcaa aaaataccaa cggaatagct
     1261 aatcccacca cta
//
LOCUS       DAOOIE010000154         1272 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_89324_length_1272_cov_2.737880, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000154 DAOOIE010000000
VERSION     DAOOIE010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89324_length_1272_cov_2.737880"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PK131_03355"
     CDS             complement(<1..363)
                     /locus_tag="PK131_03355"
                     /inference="COORDINATES: protein motif:HMM:NF019138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45188.1"
                     /translation="MIKPDTNKVEVAIKPASPQTIVNNIPPQRIEPITGVLEVMNDGQ
                     GFVRPKFTPGSRDAVIGSNLVRRYGLRPGDLIVGAAKAPKENEKYWGLIRIETVNGLS
                     LAEALHRPDFKDLVPIYPN"
     gene            complement(480..>1272)
                     /locus_tag="PK131_03360"
     CDS             complement(480..>1272)
                     /locus_tag="PK131_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HPD45189.1"
                     /translation="MIVSADAYGPIVDNAKGCAEAGGEDKKTIAVCDKLDAAGNTAKA
                     ITKGFAIGAAALTVLALFSVYTQTTGIKTLDLTNPGVIIGLFLGGMMPPLFSALTILA
                     VGRNAFLMVKEIRRQFKEIKGLVEGKCKPDSQKCIDIATTGALRELIVPGLLAFVVPL
                     IVGFGLGPNALGGFLAGSIVVGIIFALFMANAGGLWDNAKKFIEDGAFGGPGSEAHKA
                     AVVGDTVGDPFKDTAGPSINTLITVMSLVATIFSPLIVKWALFSL"
BASE COUNT          391 a          310 c          202 g          369 t
ORIGIN      
        1 attaggataa ataggtacca gatccttaaa gtccggccga tgaagagctt cagccagaga
       61 cagaccatta actgtttcaa ttctaatcaa accccagtat ttttcgtttt cttttggggc
      121 tttggccgca ccaacaatta aatcaccggg ccgtagtcca tatcgtctaa ccaaatttga
      181 accgataacc gcatctcggg atccaggggt aaatttagga cggacaaaac cctgaccatc
      241 attcattacc tccaaaaccc ccgtaattgg ctcaattctt tggggtggaa tattattaac
      301 tatagtttgt ggtgaagctg gctttattgc tacctcaacc ttattggtgt ctggttttat
      361 catttagttg gaaaaaatat ataaaaaagc ctgcaaatct ctttgggaaa cctctgatta
      421 taccacaggt atttttcctt tttcaaggga agaaacctgt caaaggcgga aaaggagttc
      481 tacaagctaa acaatgccca tttgactatt aaaggactaa atatagtcgc tactaacgac
      541 ataactgtaa ttagggtatt tatcgatggt ccagccgtat ctttaaatgg atcaccaaca
      601 gtatcgccaa ccactgccgc tttatgtgct tcactgcctg gcccaccaaa agcaccatct
      661 tcgataaatt ttttggcatt atcccacaaa ccaccagcat tggccataaa taaggcaaat
      721 ataattccta ccacaattga acctgcgaga aatccaccca aagcatttgg tccaagccca
      781 aaacccacta tcaatggaac tacaaaagcc aacaatccgg gcacaatcaa ttctctcaaa
      841 gccccagttg tcgcaatgtc aatacatttc tgactatcag gtttacattt tccttcaact
      901 agtcctttaa tttctttaaa ctgtcttcga atttctttaa ccatcaaaaa agcatttctt
      961 cctacagcca aaattgtcaa agctgaaaac aatggtggca tcattccacc taaaaataaa
     1021 cctattatta ctccgggatt agtcaaatcc aacgttttta ttccggtagt ttgggtataa
     1081 accgaaaata aggctaaaac tgttagggct gccgctccaa ttgcaaaccc cttagtaatt
     1141 gccttagccg tatttccagc cgcatctaat ttatcacaaa ctgcaatcgt ctttttatcc
     1201 tctccaccgg cttctgcaca tcctttggca ttatctacaa ttggtccata agcatcagca
     1261 ctcactatca tt
//
LOCUS       DAOOIE010000155         1268 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_89802_length_1268_cov_2.309975, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000155 DAOOIE010000000
VERSION     DAOOIE010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89802_length_1268_cov_2.309975"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="PK131_03365"
     CDS             <1..788
                     /locus_tag="PK131_03365"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPD45190.1"
                     /translation="AIISFAVAGVIPIFIEQTSAFFQALPGIFKSLKDIRLFEAIPLE
                     ASNNFNFLGDLSGGITRTVVSIFSNLFSFILILIITFYLLLERKNVETYVGRFWGRRG
                     VAKAQEFMTLLEIRLGHWVNAQLLLMLIIGLLSYLGYLLIGLNYALPLAIIAALLEIL
                     PNIGPTLATILAGIVGVTMSPLTALSAIIVGLVVQQLENGFITPKVMKDQVSLPPLLT
                     IFILLAGAKLGGLGGAILSIPIYLTFEVIYKVFVRGTVKPNSV"
     gene            complement(1032..>1268)
                     /locus_tag="PK131_03370"
     CDS             complement(1032..>1268)
                     /locus_tag="PK131_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45191.1"
                     /translation="SLLFNNSSLFQLLIFKWEDKMPTITFYSVKSRKSIDIDVAKVKK
                     VILKNGRPAAEAIDPETGTKMFKILSAADAALLK"
BASE COUNT          300 a          246 c          257 g          465 t
ORIGIN      
        1 cagcaattat tagttttgcc gtggctggtg ttattcctat ttttatcgaa caaactagtg
       61 ctttttttca agctttgcca ggaatcttta aaagtttaaa agatattaga ctatttgagg
      121 caatacctct tgaagcatcc aataatttta actttttggg tgatctttcc ggtggtatta
      181 cccgaacagt cgtttctatt ttctctaatt tatttagttt tattttgatt ttaattatta
      241 ctttttattt gcttcttgaa cggaaaaatg tggaaaccta tgtcggccgt ttttggggtc
      301 ggcggggagt agctaaagcc caggaattta tgactttgtt ggaaattcgg ttgggtcatt
      361 gggttaatgc ccaattactt ttaatgctga ttattggtct cttgtcttat ttaggttatc
      421 ttttgattgg tcttaattat gctttgccat tagcgattat agcagcctta ttagaaattt
      481 tacctaacat tggaccaacc ttggcaacta ttttggcggg tattgttggg gtaacaatgt
      541 cacccttaac agctttgtcg gcaatcattg ttggtttagt ggttcagcaa ttagaaaacg
      601 gctttattac ccctaaagtc atgaaggatc aggttagctt gccgcccctg ttaactattt
      661 ttattctttt agccggggct aaactcggcg gcttaggggg agcaatttta agcattccga
      721 tctaccttac ctttgaagtg atttacaaag tttttgttcg gggaaccgtt aagccgaatt
      781 ctgtttaaga gtcatcaatc ttgtctctct acctgaagct ctcgaaagcc agatgttatt
      841 gcttctgttt gagatttctg ccggaaggtc actcgtttca cctcctccgc caagagcgga
      901 ttcctcgtct ctttagtggt gcctagggct tacgccccac cctttcttgg ttccgtgctt
      961 tctgcagttc ggactttcct tccgctaaat agcggattga ctcttcggtt ccccaaaccg
     1021 gttattatac cttattttaa taaagccgca tcggctgcag acaagatctt gaacattttg
     1081 gttccagttt cgggatcaat agcctctgct gccggccgac cgtttttgag gattactttt
     1141 ttaactttag caacgtcaat atcgattgat tttctcgatt tgactgaata aaacgttatg
     1201 gttggcattt tatcctccca cttaaaaatt aataattgaa atagtgatga gttgttaaaa
     1261 agaagcga
//
LOCUS       DAOOIE010000156         1264 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_90224_length_1264_cov_3.114144, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000156 DAOOIE010000000
VERSION     DAOOIE010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90224_length_1264_cov_3.114144"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(139..567)
                     /locus_tag="PK131_03375"
     CDS             complement(139..567)
                     /locus_tag="PK131_03375"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPD45192.1"
                     /translation="MEDIALELIETLFQIARLVKNKISFTNNSTCLSALQLYALRYID
                     KHQEASMGDIADYFTIELPSATSLINKLVKDKLIVRCEGKNDRRKVRLILTPKGRKTL
                     QRIQNCSKYNLRRVLTDLSEKEQLDLLSILQSLQEKLKNQ"
     gene            634..>1264
                     /locus_tag="PK131_03380"
     CDS             634..>1264
                     /locus_tag="PK131_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPD45193.1"
                     /translation="MIKLLRFLKPAGLSLIFVVILAIGQASANLYLPNLMSDIVNKGI
                     AAFNTNYIWRTGGIMALVAIVGTICAIAGIFFSSRIANGMGQRLRAEIFTKIQHFSLH
                     EFDHLSTASLITRTTNDTTQIQQLVVMTLSVIITTPITLIVGIILALDQDVGLSWILV
                     AMVPILVIIVTVLLSKTIPLFTTMQTRLDKLNLILNENLTGVRVIRAFNR"
BASE COUNT          350 a          269 c          191 g          454 t
ORIGIN      
        1 cgacaaattt aactgaagca atcgtggtgg ctcgacttgt taaattattt tttttactca
       61 ccgtttgttt attaatcaac caaccaccgc ctcccaccat caaaacaatg atgataatga
      121 tccagcctcg aattcttttc attgattttt taatttctcc tgtagggact gtaaaataga
      181 taaaagatct aattgctctt tttctgataa atcagtcaga actctcctta aattatattt
      241 actgcaattt tgaattcgtt gcaatgtttt tctcccttta ggcgtcaaga ttaatctaac
      301 ttttcgtcta tcgttcttac cctcacaacg aacaatcaat ttatccttta ccaatttatt
      361 aattaaactg gtggctgacg gtaactctat agtaaaataa tcggcaatat cacccataga
      421 tgcttcttga tgcttatcaa tataccttaa agcatataat tgcagggcac tcaagcaagt
      481 agaattattg gtgaaagata ttttattttt caccagcctg gcaatttgga ataaggtctc
      541 tattagctcg agggcaatat cttccattat attttaatta aactttctaa ctattaggta
      601 gtataatcaa tccattgtca taagtcaatc tctatgatta aacttttacg ttttcttaaa
      661 ccggctggtc tatctctgat ctttgttgtc attttagcta ttggccaagc ttctgctaat
      721 ctctacctac ccaatttaat gtctgatatt gtgaataaag gtattgccgc ctttaatact
      781 aattacattt ggcggactgg cggcatcatg gcgctcgtgg ctattgtcgg tactatctgt
      841 gctattgccg gcattttttt ctcttctcgg atagctaatg gtatgggcca acggctccga
      901 gctgaaatct ttactaaaat ccaacatttc tcccttcatg aatttgatca tctcagtact
      961 gcttccttaa tcacccgaac caccaatgac actactcaaa ttcaacaact cgtagttatg
     1021 acactttctg ttattatcac tactcctatc actttaattg tcggcatcat tttagctctt
     1081 gaccaagatg ttggtctgtc ttggatatta gtagctatgg taccgatttt agtgattatt
     1141 gtgacggttc ttctctctaa aactattccc ttattcacca ccatgcagac taggctggat
     1201 aaattaaatc taattcttaa cgaaaacctt accggagtcc gggtcattcg agctttcaat
     1261 cgaa
//
LOCUS       DAOOIE010000157         1241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_92857_length_1241_cov_3.017707, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000157 DAOOIE010000000
VERSION     DAOOIE010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92857_length_1241_cov_3.017707"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="PK131_03385"
     CDS             complement(<1..1040)
                     /locus_tag="PK131_03385"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPD45194.1"
                     /translation="MNSQRIKTLSISIIILFGLILAKFFYYQIIKSFSLREKELAQLY
                     QTEKILPLRGEIRDTTDFPLVSNVTLYQLAIYKPNLKNDLVDIFNQIDRIKPGFIKNN
                     QNAINLFAGNKNQLWYVFPTLFNQTEQESIKLAGVTFKKVQKRFYPEDKLAQPVLNGL
                     EKYYDKQLSGKTGFTLSPKDAIGNPLLTQKGWNIKSVDGLHLHTFLRRDIQFYVEKTL
                     KEGSEQYQADSAWAIVMNVQNGGIIAMANIEASPSGRNMAISDLFEPGSIFKPLVVAM
                     ALDSHQITPDYTCPRCDQDRIIGDDTITNWNGQHHPQTNLQDTIKNSDNISMTYIIDK
                     LGLERFKNYYQQL"
     gene            complement(1021..>1241)
                     /locus_tag="PK131_03390"
     CDS             complement(1021..>1241)
                     /locus_tag="PK131_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45195.1"
                     /translation="KIIYLITGFLFLLEIIFSFYYSSVIVNENRILTDSQKKLDQLLL
                     EKQSLRNQFYRLVSFSASSKPIDELSAN"
BASE COUNT          389 a          188 c          246 g          418 t
ORIGIN      
        1 agttgttgat aataattttt aaaccgttct aaacctagtt tgtctataat ataggtcata
       61 gaaatattat cactattttt aatggtatct tgaaggttgg tttgggggtg atgctgacca
      121 ttccagttgg tgatagtatc atcgccaata attcgatctt ggtcacatcg ggggcaggta
      181 taatcggggg taatttggtg actatctaaa gccatagcca ctaccaatgg tttaaaaata
      241 gatccgggtt cgaaaagatc actgatagcc atgtttcttc ctgatggtga ggcctcaata
      301 ttagccatag caataatgcc gccattttga acattcataa ctattgccca agccgaatcg
      361 gcttgatatt gttcacttcc ctcttttaaa gttttttcga cgtaaaattg gatatcacga
      421 cggagaaaag tatgtaaatg taggccatca acacttttaa tattccatcc tttttgggtt
      481 aataatgggt tgccgatggc gtcctttggg gagagagtaa aacctgtttt gcctgataat
      541 tgtttgtcat aatatttttc taggccattt aaaactggtt gagctagttt atcctccggg
      601 taaaagcgct tttggacctt tttaaaagta actccggcta atttgataga ttcctgctct
      661 gtctgattaa aaagagtggg gaagacatac caaagttggt ttttgttacc ggcaaaaagg
      721 ttaatagcgt tttgattatt ttttatgaag cctggtttaa tccgatcaat ttggttgaaa
      781 atatcaacca aatcgttctt aagattaggt ttgtaaatgg ctagctgata aagagtaaca
      841 ttactgacca gtggaaaatc agtcgtatct ctaatttccc cccggagagg taaaattttt
      901 tcggtttgat acagctgggc taattctttt tccctaagag agaaactttt aataatctga
      961 taataaaaaa atttagctag aattagacca aatagaataa taattgagat agaaagagtt
     1021 ttaattcgct gagagttcat cgatgggttt tgaggaggca gaaaaagaaa ctaaacggta
     1081 aaattgattt cttaaacttt gtttttcaag aaggagctga tctaattttt tttgcgagtc
     1141 agttaaaatt cggttttcgt taacgataac actgctgtaa taaaaagaaa atattatttc
     1201 caagagaaaa agaaagccag ttatcaaata aataattttt t
//
LOCUS       DAOOIE010000158         1215 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_96144_length_1215_cov_1.114655, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000158 DAOOIE010000000
VERSION     DAOOIE010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96144_length_1215_cov_1.114655"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>430
                     /locus_tag="PK131_03395"
     CDS             <1..>430
                     /locus_tag="PK131_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45196.1"
                     /translation="RWDRLKESFHEDYRVLLPKSAVVSFLVLASFFVLPYHWFLLLAL
                     VNFIFWGSYALIQGVRKRWVYPRATSKGKVFFSFLWLATVALGLAGLFWAPLDLPFLL
                     LAVSVIWPLVVLTALRIMEWPANILKNRLIEKAKNRRAQLK"
     assembly_gap    431..440
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <441..>1215
                     /locus_tag="PK131_03400"
     CDS             <441..>1215
                     /locus_tag="PK131_03400"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPD45197.1"
                     /translation="KDLTVIGVAGSFGKTSVKEFLYHFLAEQYGQDKVLKTERNTNTQ
                     LGVAQTILNDLTSQHLFFICEMGAYRVGEIAQSAELARPSIGILTGINNQHLSLFGSQ
                     ENIIKAKYELIQSLPPTGYSIFNGDNDYCLDLYRQTSDRKILVSSKGLRLAHWQAQEV
                     LIGKDSVSFKMANRDQEILISAKLVGRQNIVNLLLAGSAALVLGLSPNELSQAAKTLP
                     TPSGGPRMVPYRQASIINAVYSSNLDGAQVHIEHLELWSG"
BASE COUNT          291 a          255 c          285 g          374 t
ORIGIN      
        1 cgctgggacc gccttaaaga gagttttcac gaagactacc gagttctgct gcctaaatca
       61 gcggttgttt catttttggt tttagcttca ttttttgttt taccttatca ttggttttta
      121 ttattagcat tagttaattt tatattctgg gggagctatg ccctgatcca gggcgttcga
      181 aagcgctggg tttatcctcg agcaacttcc aaagggaaag ttttcttttc ctttctttgg
      241 ctggctacgg tagctctggg actggcgggt cttttctggg cgcctctgga cctgcctttc
      301 ttgctgctgg cggtatctgt gatttggccc ttagttgttt tgacggccct aaggatcatg
      361 gagtggccgg ccaacattct taagaatcgc ctcattgaaa aagctaaaaa tcgccgggct
      421 caactgaaaa nnnnnnnnnn gaaagaccta acggtgatcg gagtggctgg ttctttcgga
      481 aagacgtcag tcaaggaatt tctttatcat tttttggccg agcagtatgg ccaagataag
      541 gttttaaaga ccgagaggaa taccaacacc cagctgggcg tggctcagac tattttaaat
      601 gatctgacca gtcaacatct gtttttcatt tgtgagatgg gggcttaccg cgtcggtgag
      661 attgctcaaa gcgctgaact ggctcgtcca agcataggaa ttttaactgg tatcaataat
      721 caacacctct cgctcttcgg ttctcaggaa aatattatta aggctaagta tgagttaatc
      781 caatctttgc caccgacagg ttattctatt tttaatggcg acaatgatta ttgtcttgac
      841 ctttatcgcc aaacatccga tcgaaaaatt ctagtttcgt ctaaggggtt aaggctggct
      901 cattggcaag cccaagaagt tttaattgga aaagactcag ttagctttaa gatggctaac
      961 cgagaccagg agatattaat ttcggctaaa ttggttggcc gacaaaacat cgttaatctc
     1021 ctgttagccg gttcagcggc cttagtttta ggcttgtcgc cgaatgaatt gtcccaggca
     1081 gctaaaactc ttcccacgcc ttctggcggt ccaaggatgg tgccttatcg gcaagccagt
     1141 attattaatg ctgtttactc cagtaatctt gacggagctc aggttcacat tgagcacctt
     1201 gaattatggt cggga
//
LOCUS       DAOOIE010000159         1214 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_96183_length_1214_cov_2.862813, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000159 DAOOIE010000000
VERSION     DAOOIE010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1214)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96183_length_1214_cov_2.862813"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            32..301
                     /gene="rpsO"
                     /locus_tag="PK131_03405"
     CDS             32..301
                     /gene="rpsO"
                     /locus_tag="PK131_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012242754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPD45198.1"
                     /translation="MENYKEEKADIIRKFAQNKGDTGSPEIQVAILTKEIEKLQFHLT
                     ENKHDTPAKRGLLTKIAKRRRLLRYLSSHAAERYGKLIKELDLKK"
     gene            306..>1214
                     /locus_tag="PK131_03410"
     CDS             306..>1214
                     /locus_tag="PK131_03410"
                     /inference="COORDINATES: protein motif:HMM:NF013318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPD45199.1"
                     /translation="MEKKIMKLSLGGADFTLEAGILAPQTNGSIIARWGGTVVLATVV
                     LGELDETKDYFPLSVEYADRLYAGGLIKGGKWMKREGAPSDAAILFGRIIDRSLRPLF
                     PNGFHHEVQIITTVLSNDKEHDPVVPAFLAAAAALTASDIPFDGPVAIVRVGLINHEL
                     KLFPSVSELNQSELDLLVCTGPAGVNMIEAGAQMVDNELAYQAIELAKNNGEEINDQI
                     KHFTASFQKSKIAFEPYLPTKELVDQLEGQIKDEVEEFLDHGADGKHIEGEKKLVEKI
                     LALYDDQIKAEEVNPNILKEALAQIVK"
BASE COUNT          403 a          198 c          267 g          346 t
ORIGIN      
        1 tgattttgtt tattgataaa acattaaaaa aatggaaaat tataaagaag aaaaagcgga
       61 tattattaga aaatttgccc agaataaagg ggataccggt tcgcctgaaa ttcaagtagc
      121 gattttaact aaagagatcg aaaaactgca atttcatttg actgagaata aacatgatac
      181 tccggctaag cgtggtttat taactaaaat agccaagcgc cgccggttat tgcgttatct
      241 ttcctctcat gccgccgaac gttacggtaa actaatcaaa gaacttgatt tgaaaaaata
      301 aaaatatgga gaaaaaaatc atgaagcttt ccctaggggg agcggacttt accctagagg
      361 ccgggatttt agcacctcaa actaacggat caataatagc tcgttgggga ggaacggtgg
      421 tcttagccac ggtagtctta ggagaattag acgaaactaa ggattatttt cctttgtcgg
      481 tagaatatgc tgatagatta tatgccggcg gtcttattaa aggcggtaaa tggatgaaac
      541 gggaaggagc accctcggat gcggcgattc tttttggccg gattattgat cgttctttgc
      601 gacctttatt tcccaatggt tttcaccatg aagttcagat aattactact gttttaagta
      661 atgataagga acatgatccg gtagtacctg cctttttagc ggcggcggcg gctttgactg
      721 cctccgatat tccttttgat ggacccgtag caattgttcg ggtaggtttg attaatcatg
      781 aattaaaatt atttccatca gttagcgaat taaatcaatc agagttagat ttattagtgt
      841 gtaccggccc ggctggagtc aatatgattg aagccggcgc ccaaatggtt gataatgagt
      901 tagcttatca ggcaatagaa ctggctaaaa ataatggcga agaaattaat gaccagatta
      961 aacactttac ggcttcattt cagaaatcaa aaatagcttt tgagccatat ctgccaacaa
     1021 aagaattagt tgatcaattg gaaggtcaaa ttaaagatga agtcgaagaa tttttagatc
     1081 atggtgctga tggtaagcat attgaaggtg aaaaaaaatt agtagagaag attctagccc
     1141 tttatgatga ccaaataaaa gccgaagaag tcaaccccaa tattttaaag gaagctttgg
     1201 ctcaaattgt gaaa
//
LOCUS       DAOOIE010000160         1211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_96622_length_1211_cov_1.557093, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000160 DAOOIE010000000
VERSION     DAOOIE010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96622_length_1211_cov_1.557093"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1211)
                     /gene="dnaK"
                     /locus_tag="PK131_03415"
     CDS             complement(<1..>1211)
                     /gene="dnaK"
                     /locus_tag="PK131_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011526995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPD45200.1"
                     /translation="SPQEVSAMILSKLKADAEAKIGEKITEAIITVPAYFDDSQRQAT
                     KDAGLIAGLDVKRIINEPTAAALAYGFDKKQGQKIVVYDLGGGTFDVSVLDISTDTVE
                     VKATNGNTHLGGEDFDKIIIDWIISEFKKDQGIDLSKDTLALQRIKESAEKAKIELST
                     AMETEINQPFITTDQNGPKHLVMKLSRAKLEVLVGDLVAKTIEPCKKAIADSGFSLSD
                     INEVIMVGGMTRMPLVQQKVKEFFGKEPNLNVNPDEVVAMGAAVQAGVLQGDVKDVLL
                     LDVTPLTLGIETLGGVATPLIERNTTIPTSKSQIFSTAADGQTSVEIHVVQGERPMAA
                     DNKSLGRFILDGIPSAPRGVPQVEVKFDLDANGILNVSATDKATGKQQNITITASSGL
                     SKEEIEKMKREA"
BASE COUNT          334 a          295 c          239 g          343 t
ORIGIN      
        1 gcttctcttt tcatcttttc aatttcttct ttggacaagc cggaagaggc ggtaatcgta
       61 atattctgct gcttgccggt ggctttatca gtcgcgctca catttaagat gccgttggca
      121 tccaaatcaa attttacctc aacttgaggt accccgcggg gagctgaagg aataccatcg
      181 aggataaaac gtcccagaga tttattgtca gccgccatcg gccgctcacc ttgaacaaca
      241 tgaatttcca cactagtctg accgtcggcc gcggtggaaa aaatctgcga tttagaagta
      301 gggatagtgg tattgcgttc aattaacggt gtagcaactc cacctaaagt ttcaatcccc
      361 aaggtaagag gggtgacgtc aagcaagagc acatctttaa cgtcgccctg aagaacaccg
      421 gcttgaacag ccgcgcccat cgccacgact tcatcaggat tgacgtttaa atttggttct
      481 ttgccaaaaa attcctttac cttttgctgg actaaaggca tacgggtcat accgccgacc
      541 ataattactt cattgatatc acttaagctg aaaccagaat cagctatcgc tttcttacaa
      601 ggctcaattg tcttcgccac caagtcgccc actaaaactt cgagtttggc gcgacttagc
      661 ttcataacta aatgtttagg cccattctga tcagtggtaa taaaaggctg attgatttcg
      721 gtttccattg ccgtagataa ttctatttta gctttttccg ccgattcttt aatccgctgc
      781 aaagctaaag tatctttaga caaatcaata ccctgatctt ttttaaattc agagataatc
      841 caatcaataa taatcttatc aaaatcttct ccgcctaaat gagtattacc atttgtagct
      901 ttaacctcca ccgtatcagt agaaatatca agcacagaga catcgaaagt tccgccgccc
      961 aaatcataga caacaatctt ctgcccctgt ttcttatcaa agccgtaagc caaggcggcg
     1021 gctgtcggtt cattaatgat acgcttgacg tccaaaccgg caataagccc ggcatcttta
     1081 gtcgcttggc gctgagagtc atcaaaataa gcgggaacgg taatgatagc ttcagtaatt
     1141 ttctcgccga tttttgcttc cgcgtcggct ttaagcttag acaagatcat agccgaaact
     1201 tcttgaggag a
//
LOCUS       DAOOIE010000161         1189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_99358_length_1189_cov_4.112875, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000161 DAOOIE010000000
VERSION     DAOOIE010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99358_length_1189_cov_4.112875"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            37..>1189
                     /locus_tag="PK131_03420"
     CDS             37..>1189
                     /locus_tag="PK131_03420"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPD45201.1"
                     /translation="MTNTAPTVYRNISEVLLAKNLINQEQAQEIGMRQLKTGDSEETI
                     IKRLRLVSDRDFVEAKAEYLRIPFIDLDKIGFSPEALSLVPEVVAKKYLVAPYKLDPK
                     NKVLYVSMVNPLDLETIEFLEKKSGMRVVAGMSVQKQIDDFIGEKYTREKGIATEVGK
                     AVEEQKKEEKAVIEEAEEQVSAEAPVAKIVATILEYAARARASDIHIEPLEENVRIRY
                     RIDGILQEKYLLPRNVHDAVVSRIKILSGMKIDEKRVPQDGRFYFTYQGNEIDLRVST
                     IPTTYGEKVALRLLQKSIKTPTLPELGLRGLALKNLMTAIERPHGIIIVCGPTGSGKT
                     TTLYSILGIVATPKVNVVTIEDPVEYQMKGVNQVQVNVQAGLTFASALRS"
BASE COUNT          363 a          190 c          299 g          337 t
ORIGIN      
        1 tttaaggaaa aagggtcttc atgtagtatt aattatatga cgaatactgc cccaacggtt
       61 tatcgaaata tcagcgaggt tttattggct aaaaatttga ttaatcaaga gcaggctcag
      121 gaaataggaa tgcgccagct gaaaaccggt gatagtgagg agacaattat taaacggctg
      181 cggctggtta gcgatcgtga ttttgttgaa gctaaggctg aatatttaag aatccctttt
      241 attgatcttg ataaaattgg tttttcaccg gaagctctat cgttggtgcc agaggtagta
      301 gctaaaaaat atttagtggc tccttataaa ctggatccta agaataaagt tttgtatgtc
      361 agcatggtta atccattgga tttggagaca attgagtttt tggaaaagaa aagtggcatg
      421 agggtggtag ccggaatgtc ggttcaaaaa cagatcgatg attttatcgg ggaaaagtac
      481 accagggaaa aagggattgc cactgaagtt ggcaaggccg ttgaggagca aaaaaaggaa
      541 gaaaaagcgg ttattgagga agcggaagag caggtgtcgg ccgaggctcc agtagccaaa
      601 attgtcgcta ctattttgga atatgccgcc agagccagag cttccgatat tcatatcgaa
      661 ccattagaag aaaatgtaag aatacgttat agaatagacg gtattttaca ggaaaaatat
      721 ctgttacccc gtaatgttca tgatgctgtt gtcagccgga ttaagatcct ctccgggatg
      781 aaaattgatg aaaagcgggt tcctcaggat ggtcgatttt attttactta tcagggtaat
      841 gaaattgatt tgcgggtctc aactattcca acaacttatg gtgaaaaagt tgctttgaga
      901 ctattacaaa aatcaatcaa aaccccgacc ttgcccgaac tgggtttaag aggattggct
      961 ttgaaaaatt tgatgacggc gattgaacga cctcatggga ttattattgt ctgtgggccg
     1021 actggttcgg ggaaaacgac gactttgtat tctattttgg gaattgtggc tactcccaaa
     1081 gtcaatgtgg tgacaattga agatccggtg gaatatcaaa tgaaaggtgt taatcaggtt
     1141 caagttaacg ttcaggctgg cctgacgttt gcctcggctc ttcggtctt
//
LOCUS       DAOOIE010000162         1187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_99682_length_1187_cov_2.444346, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000162 DAOOIE010000000
VERSION     DAOOIE010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99682_length_1187_cov_2.444346"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1187)
                     /locus_tag="PK131_03425"
     CDS             complement(<1..>1187)
                     /locus_tag="PK131_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPD45202.1"
                     /translation="SLGRHFDMPAIFQLSTGVSSSDLADQVCFTVDASVKEKQAMLET
                     FLVSQRLRLATEYLAREIKVAEVEMDIEKKTQEKFNDSMKRTVLEERKHQIEKELEKL
                     GQKSEHEYSDLEKKIRQAKMPPGVKKKCLKELNRLLEMGVYAPESSYLRTYLETVVEL
                     PWGKYSKSVVKITKAKKILNQDHYGLQDVKERILEYLAVLKLRSIKKHDDNTGGSANI
                     LCFIGPPGVGKTSLGRSIARALNRKFIRASLGGVRDEAEIRGHRRTYVGALPGRIVKG
                     IKDCGSMNPVFMLDEIDKIGTDFRGDPSSALLEVLDPEQNREYTDHYLDFPLDLSKVF
                     FILTGNDVSTIPGPLRDRIEIIPFSSYTLTEKFHIAKKYLIKKELAANGLISKNLRPI
                     KDET"
BASE COUNT          353 a          265 c          187 g          382 t
ORIGIN      
        1 gtctcatctt taattggccg aagattttta gaaattaacc cattagccgc taattccttt
       61 tttattaaat attttttagc aatgtgaaat ttttcggtta aggtgtagct cgaaaaggga
      121 attatttcaa tccgatcccg caatggtccc gggatagttg aaacatcatt accagttaaa
      181 ataaaaaata ctttggataa atcaagggga aaatcaagat aatgatcggt atattcacgg
      241 ttttgttcag gatcaagaac ctcaaggaga gcgcttgagg gatcaccccg aaaatcggtt
      301 ccaattttat caatttcatc caacataaaa accggattca ttgaaccgca gtccttaatt
      361 cccttaacaa ttcgacccgg taaagcgcca acataagtcc gacgatgacc tcgaatttca
      421 gcttcatccc tcactccgcc caaagaggct cgaataaatt tccgatttaa agctcgagca
      481 attgatcgac caagagaggt tttaccaacc cccgggggac caataaaaca aagaatatta
      541 gccgatccac cggtattgtc atcatgcttc ttgattgatc ttaactttaa aactgccaga
      601 tattccaaaa tcctttcttt aacatcctgt aaaccatagt gatcctgatt taaaattttc
      661 ttggctttag ttatttttac taccgatttc gaatattttc cccatggcag ttccaccact
      721 gtttccaagt aggttcttag atagctagat tcaggtgcat aaacacccat ttctaataat
      781 cggtttaatt cctttaaaca tttctttttc actcccggcg gcatttttgc ctgacgaatt
      841 ttcttttcca aatcactata ttcgtgctca cttttttgac ctaatttttc caattccttc
      901 tcaatttgat gctttcgttc ctctaaaacc gttcgcttca ttgaatcatt aaatttctcc
      961 tgggtttttt tctcaatatc catttcaacc tcggctactt taatttcccg agctaaatat
     1021 tcggtggcta atcttaaccg ctgagaaaca agaaaagttt ctaacatggc ttgcttttcc
     1081 ttaacactgg catcaactgt aaaacaaact tgatcggcta aatcagagct ggaaacaccg
     1141 gttgataact gaaaaattgc cggcatatca aaatgacgac cgagtga
//
LOCUS       DAOOIE010000163         1180 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_100550_length_1180_cov_3.584889, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000163 DAOOIE010000000
VERSION     DAOOIE010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100550_length_1180_cov_3.584889"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PK131_03430"
     CDS             complement(<1..237)
                     /locus_tag="PK131_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45203.1"
                     /translation="MVERKNFLLTKFKFLTERQKGREKPFSPQFNEENLKSDLEEATK
                     KLQAKVLVQGEPVEIITIVSGEKYQRDDRGLSLKC"
     gene            365..>1180
                     /gene="rfbB"
                     /locus_tag="PK131_03435"
     CDS             365..>1180
                     /gene="rfbB"
                     /locus_tag="PK131_03435"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HPD45204.1"
                     /translation="MKLLVTGGLGFIGSNFIHYWLRRHPDDFIVNLDKITYAANPANL
                     KDWEGNSHYQFIKGDIGDSAVCRQAMEGVEAIVHFAAESHVDRSLADPELFLQTNVLG
                     TFTLLNVAAEKKIRFHHISTDEVFGALPLDSSQRFNELTVYDPRSPYSASKAASDHLV
                     RAFYHSFGLPITISNCSNNYGPYQSPEKFIPRIITNLLTGQKVPVYGDGLYVRDWLYV
                     EDHCRAIELILMNGRIGETYCVGGMTENINNLTLTKTILEIMGKDENQIEFVAD"
BASE COUNT          318 a          225 c          231 g          406 t
ORIGIN      
        1 gcattttagg gataaacctc tatcatcccg ttgatatttt tcgccactga caatagttat
       61 aatttccact ggttcccctt gaactaagac tttagcttga agcttttttg ttgcttcttc
      121 taaatctgac ttcaggtttt cttcgttgaa ttggggcgaa aacggctttt ccctaccttt
      181 ttgtcgttcc gttaaaaatt taaatttggt gagtaaaaaa ttttttcttt caaccatgcc
      241 gcattataac ataatagaca catttaatcc tgtgaatatt tagctcttga caagcctata
      301 atattatggt ataataataa tatatcctaa acttagagaa cagcaggtgt tatactaatt
      361 ccatatgaag cttttggtta ccggtggact gggctttatc ggctcgaatt tcattcatta
      421 ttggttgcgg cgccaccccg acgattttat cgttaatttg gataaaatta cctatgccgc
      481 caatccggct aatttaaagg attgggaagg taattctcat tatcagttta ttaaggggga
      541 tattggtgat tcggctgtct gccggcaagc gatggaagga gttgaggcta ttgtccattt
      601 tgccgctgaa tcccatgttg accggtcgct ggccgatccg gaattatttt tacagaccaa
      661 tgttttagga acatttacac tgttgaatgt cgccgctgag aaaaaaattc ggtttcatca
      721 tatttctacc gatgaagttt tcggagcctt gcctttagat tcatcccaaa gatttaatga
      781 attgactgtt tatgatcctc gatcgcctta ttctgccagc aaggccgctt ccgatcattt
      841 agttcgagcc ttttaccatt cttttggatt accaattact atttccaatt gttccaacaa
      901 ttatggtcct tatcaatcac cggagaaatt tattccccga ataattacca atttattaac
      961 cggccaaaaa gtgccggttt acggcgatgg tttgtatgtc cgtgactggc tttatgtcga
     1021 agatcattgc cgggcgatcg aattaatttt aatgaatggt aggattggtg aaacttattg
     1081 cgtcggcggc atgaccgaaa atatcaataa tttaaccttg actaaaacta ttttggaaat
     1141 tatgggaaaa gatgagaatc agatcgaatt tgtggctgat
//
LOCUS       DAOOIE010000164         1165 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_102662_length_1165_cov_2.572973, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000164 DAOOIE010000000
VERSION     DAOOIE010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1165)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102662_length_1165_cov_2.572973"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="PK131_03440"
     CDS             complement(<1..991)
                     /locus_tag="PK131_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45205.1"
                     /translation="MKLKLTDWLIIIACFLVFIGWRLYPYLDNNVPLGYDPGLYLYGF
                     KNFPFPNDWLKSMYPPGLFALVHLIPFNPTYLLIPLVLFFSALLFWAVFLLANRLWGK
                     KAAFWAIFLLASSLVQYRVFYWYYLKNIAALAFLFLTFYCLLSKSYWAIFWGVLTVYF
                     HSPTDFILYAALFSGLLFAKNHRPYYLLTLVLTCLFCLPRLSSYQPLVKSILTSFGEA
                     SGTFYEPAAWLVLSILYLPLGLLTFLKNYRKIPFLILPIFGLLLTTILRMFFYRRLII
                     YLDIFLLLFAALALSKIKNRLFLIIFVILNLIYLAFFVKRTAYPQINHDEFREI"
BASE COUNT          491 a          211 c          199 g          264 t
ORIGIN      
        1 gaatttcccg gaattcatca tgattaatct gtggataagc cgtccttttg acaaaaaagg
       61 ccaaataaat caaattaaga attacaaaaa taattaaaaa caatctattt ttaatcttag
      121 acagagccaa agcggcaaac agcaataaaa aaatgtctaa ataaattatc agccggcggt
      181 aaaaaaacat cctcaaaata gtcgttaata atagcccaaa gataggcaaa attaaaaatg
      241 gtatttttcg ataatttttc aaaaaagtca acaaacccaa tggtaaataa agaatactta
      301 aaaccaacca agccgccggc tcataaaaag ttcccgaagc ttcgccaaaa ctagttaaaa
      361 ttgacttgac cagcggctga taactggaca ggcgaggtag acaaaataaa caggttaaaa
      421 ccagagtcaa caaatagtag ggccggtgat ttttggcaaa taacaaaccg gaaaataagg
      481 cggcatacaa aataaagtcg gttggtgaat ggaaataaac agtcaaaacc ccccaaaaga
      541 tggcccaata agattttgat aataaacaat aaaaagttag aaataaaaag gctaaagcgg
      601 caatattttt cagataatac cagtaaaaaa cccggtattg taccaaagaa ctggctagta
      661 aaaatatagc ccaaaatgcg gcttttttac cccataaacg attggccagt aaaaaaacag
      721 cccaaaataa taaagcggaa aaaaacaaga ctaagggaat aagaagatag gttggattga
      781 acggaattaa atgaaccaaa gcaaataatc ccggaggata catcgatttc aaccaatcat
      841 ttgggaatgg aaaattttta aaaccatata aatataaacc tggatcgtaa cccaaaggaa
      901 cattattatc cagatacgga taaagccgcc agccgatgaa aaccaaaaaa caggcaataa
      961 taatcagcca atctgtcaat tttaatttca tcggctagtc aattttaaca aaaacttcta
     1021 aattaactcc aaggggccat ttcaaccttt ttaattaaag aaaaaatcaa aactttcgtt
     1081 agggttaaaa aactcgtctt ccgccggctg gggttgagcc cccaaataaa cattgatttg
     1141 ggcggaatca gtcgctaacg gcgtg
//
LOCUS       DAOOIE010000165         1164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_102797_length_1164_cov_2.811542, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000165 DAOOIE010000000
VERSION     DAOOIE010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_102797_length_1164_cov_2.811542"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..1004
                     /locus_tag="PK131_03445"
     CDS             21..1004
                     /locus_tag="PK131_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45206.1"
                     /translation="MKKITKAKWGTPKTIITVILILLVGMGIGFAWGQTKSKNKAATS
                     ADNAPKKIQIPKSDKPELAFYVMSFCPYGNQMEDILRPVFDLIGSKAEIRPHYIFEKI
                     DNLSETCKLMAGDITKCPDYVANKYFATKEECEKVIKTNNKECNDPKNYIKSSDGVYY
                     ASLHGRVEANENIREICAYNLATDKKLWWDFISQVNKNCTSQNADSCWEEQAKKAGLD
                     SAKITECFDKDGIKLIEKEIALTTKNQITASPTVLVNGINFPPEDAYTQDGKGSVVLG
                     KVVIPQAEFRTPNTQKEALCAGFKKVPEECKTILKQDQKAAAAAATAGGCQ"
BASE COUNT          391 a          236 c          241 g          296 t
ORIGIN      
        1 ttatcagtat acttataacc atgaaaaaaa taactaaagc taaatgggga acacccaaga
       61 cgattatcac ggtgattctg atccttctgg ttggaatggg aatcggtttt gcctggggac
      121 agacaaaatc taaaaataaa gccgctactt cggccgataa tgccccaaaa aaaattcaaa
      181 tccccaagag tgacaagcca gaattagctt tttatgttat gagtttttgc ccttatggca
      241 atcagatgga agatattctc cggccggtat ttgacctaat cggcagtaag gccgagatta
      301 gacctcatta tatttttgaa aaaattgata atctgagtga aacctgtaaa cttatggccg
      361 gtgatattac taaatgtccc gattatgtcg ccaataaata cttcgcaact aaagaggaat
      421 gtgagaaagt aatcaaaacc aataataaag aatgtaatga tcctaaaaat tatattaaat
      481 cttctgacgg ggtctattat gcctctctcc acgggcgggt agaagcgaac gaaaacatcc
      541 gggaaatctg tgcttataat cttgccactg ataaaaaact gtggtgggat tttatcagcc
      601 aagtcaacaa gaactgcacc agccaaaatg ctgacagttg ctgggaagaa caggccaaaa
      661 aagcaggttt ggattcggcc aaaattactg aatgtttcga taaagacggt attaagctga
      721 ttgaaaagga aattgccctc accactaaaa atcaaattac ggcttcacca accgttctag
      781 ttaatgggat taatttccct ccggaagacg cttatactca ggatggcaaa ggcagcgttg
      841 tcttaggcaa agtcgttatt cctcaagccg aatttagaac tcctaacacc caaaaagagg
      901 ctctctgtgc cggctttaaa aaggtgccgg aggaatgtaa aacaatcctt aaacaggatc
      961 aaaaagcagc agccgcggcg gctacagccg gtggctgcca ataacctggc gtgagcttaa
     1021 attctcatca attaagaaaa acccaaacgg ccgaagccga agtaataagt caaaacctaa
     1081 aaaggcagcc ctttgtctta gttttagata atgttttaga tacctacaat atcggcagtt
     1141 ttttccgtct ggctgatgct gtcg
//
LOCUS       DAOOIE010000166         1155 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_104167_length_1155_cov_2.223636, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000166 DAOOIE010000000
VERSION     DAOOIE010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104167_length_1155_cov_2.223636"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="PK131_03450"
     CDS             <1..441
                     /locus_tag="PK131_03450"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPD45207.1"
                     /translation="SVNAGDRIATVISGSSCNSSGTHLHFMVQKDGIVQNPFNFLKPV
                     DYNNDSGGDPFNPTGSWNWPLSPKIKFNQGYGRTWAVENTWVKRIYSFHNGIDIGSDN
                     LAVFATHNGTLYRGSFKGNCILKYVRVEDEGSKTETYYLHINYF"
     gene            complement(508..1125)
                     /locus_tag="PK131_03455"
     CDS             complement(508..1125)
                     /locus_tag="PK131_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HPD45208.1"
                     /translation="MKIIAKLILTFLIFSFLGAPKTVLAQYCCHEERSVSIDKKVRGI
                     SMDHYYDNLDEGTIRFKNGDLIEFSIRVENTGPIDLNNIEVRDILPTGLDLKLYDGQF
                     NQVENKIVWRIDRLTPTESRSFTVRAQIKEADKFPGQYTNIAEVTSDNSIGDRDTASY
                     YAGAQGTTLVQRVIPKTGSGLIFGSLAALVTIGGAIALRKWSRGY"
BASE COUNT          353 a          256 c          206 g          340 t
ORIGIN      
        1 agtgtcaatg ctggtgaccg aattgccact gttatttctg gcagttcttg caacagcagc
       61 ggcactcacc ttcattttat ggttcagaaa gacggaattg ttcaaaatcc ttttaatttt
      121 ctcaaaccag tggattacaa taatgactct ggcggcgacc cctttaatcc cactgggtca
      181 tggaattggc ctttgtctcc caaaatcaaa tttaatcaag gctacggccg cacttgggca
      241 gtggaaaaca cttgggtcaa acgaatctat tcttttcaca acggcatcga catcggcagc
      301 gacaatttgg ctgtttttgc tacccacaac ggcactcttt accgcggcag tttcaaagga
      361 aattgcattc taaaatacgt ccgtgtcgaa gatgaaggca gtaaaaccga gacttactat
      421 ctgcacatta attacttcta aaagcaaaat ggtcttgaca aacagcctat aatatgatat
      481 aattaagggt tggcgggcag gtttaatcta gtatcctcga ctccatttac gaagagcaat
      541 agcgccgccg attgtaacca acgccgcaag agaaccgaaa attaaaccac tacctgtttt
      601 aggaataacc ctttgaacca aagttgtccc ttgggcgccg gcataatagg aagcagtatc
      661 ccggtcgccg atgctgttat ccgaagttac ttcggcaata ttagtgtatt gaccggggaa
      721 tttatccgct tctttaattt gggctctgac cgtaaaagat cttgattccg tcggtgttaa
      781 tcggtcaatc cgccaaacaa ttttattctc aacttgatta aattgaccat cataaagctt
      841 taaatctaaa ccagtaggaa gaatatccct aacttcaata ttatttaaat caatcggacc
      901 ggtattttcc actctgatgc taaactcgat taaatcacca tttttaaacc gaatcgtccc
      961 ctcatctaga ttgtcataat aatgatccat acttattcct ctcacttttt tatcgataga
     1021 aacagaccgt tcttcatgac aacaatactg agccaaaact gttttaggcg ctcctaaaaa
     1081 actgaatatc agaaaagtta aaatcaattt tgctataatt ttcattacag gatgaattgt
     1141 agctaaaaaa tttta
//
LOCUS       DAOOIE010000167         1151 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_104694_length_1151_cov_3.252737, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000167 DAOOIE010000000
VERSION     DAOOIE010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104694_length_1151_cov_3.252737"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PK131_03460"
     CDS             complement(<1..354)
                     /locus_tag="PK131_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45209.1"
                     /translation="MTNEDRINLITRNCEEIITPEDLEKLVESGRPIKHYIGFEISGL
                     VHLGTGLMAMGKIADFVQAGVDCTILLADYHSFLNNKLGGDLAEIRWAAENYFKEALI
                     ASLKCFNIEAGQNLGR"
     gene            complement(397..>1151)
                     /locus_tag="PK131_03465"
     CDS             complement(397..>1151)
                     /locus_tag="PK131_03465"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HPD45210.1"
                     /translation="PQEAQEWEKIVKKNYRKEARVLYEFKKDLTDLLAKNPPIGGGQY
                     EVQARIKGLYASYLKTQHKGEIDLKDRIGFRVITQNITDCYRVLGVLHSNYPYVKEEF
                     NDYISVPKDNGYQTIQTTLQWKHKLWVEVQIRTEEMHQLAEFGPASHTIYKIGDGRGL
                     NPKYSWIRKLIQWQKNTDNVNNYQVNVLANFIYVFTPKGDIIELPAGATVKDFAYKIH
                     TELGKKCKKGKVNGKIVDKYQVLKTGDKVEAI"
BASE COUNT          332 a          254 c          183 g          382 t
ORIGIN      
        1 acgccccaga ttttggccgg cttcaatatt gaaacatttt aacgaagcaa ttaaagcttc
       61 cttaaaataa ttttccgccg cccatctaat ttctgctaaa tcaccgccta atttattatt
      121 aaggaaggaa tggtaatcag ccagcaaaat agtacagtca acgccggctt ggacaaaatc
      181 ggctatttta cccatggcca tcagcccggt gcctaaatga actaaaccgg agatttcaaa
      241 accaatataa tgtttgatcg gccgaccaga ttcgactaat ttttccaagt cctccggggt
      301 aattatttcc tcacaattac gggtaattaa attaatccta tcctcattag tcataaaatc
      361 attataacaa attaaaccaa tatcccctca tttcattcat attgcttcca ctttatcccc
      421 ggtttttaag acctgatatt tatcgactat tttgccgttg accttgccct ttttacattt
      481 tttccccaat tcagtatgaa ttttgtaagc aaaatccttc acggtagcgc cggcaggcag
      541 ttcaataatg tcgccctttg gagtaaaaac ataaataaaa ttagctaaaa catttacctg
      601 ataattattg acattatcgg tatttttttg ccattggatt aattttcgaa tccagctgta
      661 ttttggatta aggccgcggc catcaccaat tttgtaaatt gtgtgggaag ccggcccaaa
      721 ttcggctaac tggtgcattt cttccgttct aatctgaact tccacccaaa gcttgtgttt
      781 ccactggaga gtagtttgaa ttgtctgata accgttatct ttaggcactg aaatataatc
      841 attaaattct tccttaacat agggataatt agaatggagc acccccaaaa ctcggtagca
      901 gtcggttata ttttgggtaa ttactcgaaa accaattctg tcttttaagt caatttcacc
      961 cttatgctgg gtttttaaat agctggcata taaaccttta atccgggcct ggacttcata
     1021 ctgcccaccg ccaattggtg gatttttagc taataaatcg gttaaatctt tcttaaattc
     1081 atacaaaacc cgggcctctt ttcgataatt ctttttaaca attttttccc attcctgagc
     1141 ttcctgagga t
//
LOCUS       DAOOIE010000168         1137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_106791_length_1137_cov_2.482440, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000168 DAOOIE010000000
VERSION     DAOOIE010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106791_length_1137_cov_2.482440"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PK131_03470"
     CDS             <1..582
                     /locus_tag="PK131_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPD45211.1"
                     /translation="RSISIMGKAKGKEVDMATLIYPPLQVADIFTLDIDLAHAGMDQR
                     KAHVVARDVAKKLKWKPPIAIHHNLVAGLKAATSDLKMSKSKPESAIFIHDSPEEIRA
                     KIKKAYCPPAIIDNNPMINWLKTLMFWGEETAEYKQLIKDYQSGRLHPLDLKEKIGDW
                     LIKKLAPARAHFEIEKNKQSLAQMKSDCFPKNL"
     gene            complement(569..1003)
                     /locus_tag="PK131_03475"
     CDS             complement(569..1003)
                     /locus_tag="PK131_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45212.1"
                     /translation="MKLFLNILLILFLPLTIYAIIQNTKGTTVYNVKAERFSPVKESE
                     TSDPYFKHIRRALDGYLNGTNDGISLPTFVVNPSTDSNGKMFGLASFNRLYYTSDFTV
                     LKVENAKPYGKIITIFFDKMPDKIFDCWVLKSAKGEYDLRGF"
BASE COUNT          356 a          229 c          243 g          309 t
ORIGIN      
        1 cggtcaattt ccattatggg caaagccaaa ggcaaagagg ttgatatggc tactttaatt
       61 tatccgcctt tacaggtggc cgatattttt actttggata tcgacctggc ccatgccggt
      121 atggatcaaa ggaaggctca tgtggtggca cgggatgtcg ccaaaaaact aaaatggaag
      181 ccgccgattg ctattcatca taatttagta gcggggctga aggcggcaac ttcagattta
      241 aaaatgagca aatcgaagcc ggaatcagcg atttttatcc acgacagccc ggaagaaatc
      301 agagccaaaa ttaaaaaggc ttattgcccg ccggcaatta ttgacaacaa cccaatgatt
      361 aattggctaa aaaccttgat gttttggggc gaagaaacag ccgaatacaa gcaattaatt
      421 aaagactatc aaagcggccg tctccatcca ctagatctaa aggaaaaaat cggcgattgg
      481 ttgatcaaaa aattagcccc ggctagggct cactttgaaa ttgagaagaa taaacagtca
      541 cttgctcaga tgaagtcgga ttgttttcct aaaaacctct gaggtcatat tcgcctttgg
      601 cgctttttaa gacccaacag tcgaagattt tatccggcat tttatcaaaa aagatagtaa
      661 taattttgcc gtatggtttg gcattctcaa cttttaaaac cgtgaaatcg gaagtataat
      721 agagccggtt aaaagaagcc aaaccgaaca ttttgccgtt gctgtcggtg ctgggattaa
      781 ctacaaaagt cggcagacta atgccgtcgt tggtgccgtt taaataacca tcaagcgccc
      841 ggcggatatg cttaaaataa ggatcgcttg tttccgactc cttcaccggt gaaaaacgct
      901 cggccttgac gttataaacc gttgttcctt tggtgttttg gatgatagcg tagatagtca
      961 gaggaagaaa aagaattagc aaaatattaa gaaacaattt catgctctac attcatttta
     1021 cactaaataa atcccactat taggccccct tttgtcaaag ggggcaaatc ggtaatccga
     1081 ttaggggatt tttaattgaa tttcaccgct ccatcattat aaacctgttt tacttgt
//
LOCUS       DAOOIE010000169         1135 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_107091_length_1135_cov_2.749074, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000169 DAOOIE010000000
VERSION     DAOOIE010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107091_length_1135_cov_2.749074"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1120)
                     /locus_tag="PK131_03480"
     CDS             complement(<1..1120)
                     /locus_tag="PK131_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012430.2"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HPD45213.1"
                     /translation="MPKYKKPAVEVIMTTLHSGGKFDGTAYKISGGLHGVGAACVNAL
                     SQLYQIEIRRQHKIYWMEFVRGEVKTKLTEIPETKVALLKDLIPKEITGTTVTFYPDP
                     EIFKETLDFDPKIIIKSLKDRAYLTPKIYFHYFNEIHHSEKHYYFEGGIISLLRELNK
                     NKTTLHEPIFVHGAEEEIDVEVAIQYNDTFQENIRSYVNIINTAEGGTHLTGFKTALT
                     KVIKDYAIKKEMLKNKEETITGDDVREGLTAVLSLKMPSKNLQFEGQTKAKLGNAEVQ
                     PVVQKIVREQLEMYFEEHPETAKAIVNKSLLAIQIHRAARAAKDAIMRKGIFESLGLP
                     GKLADCQSKHPEESELYIVEGDSAGGSAKQGRDRRFQAI"
BASE COUNT          307 a          225 c          239 g          364 t
ORIGIN      
        1 ggattgcctg aaaacggcgg tctctcccct gcttggcact accgccggcc gaatcaccct
       61 cgacaatgta aagttcactt tcttcaggat gcttagattg gcagtcagct aatttgcctg
      121 gaagaccgag agattcgaag atgcctttgc gcatgatggc atctttagcc gcccgagcag
      181 cccggtgaat ctgaatggct aaaagtgatt tgttgacaat ggctttagca gtttccggat
      241 gctcttcaaa atacatttct aattgctccc gaacgatttt ttgaactacc ggttgaacct
      301 cggcgttgcc gagcttcgcc ttggtttggc cttcaaattg aagatttttt gagggcattt
      361 ttagagataa aacggcggtc aaaccctcgc gaacgtcatc accggtaata gtttcctcct
      421 tatttttcaa catttctttt ttaatcgcat agtctttaat taccttggtt aaggcggttt
      481 taaaaccggt taaatgagtt cccccctcgg ccgtattaat aatattgacg taagatcgaa
      541 tattttcttg gaaagtatca ttatattgga tagctacttc aacgtcgatt tcctcttcgg
      601 caccatggac aaaaattggc tcgtggagag tggttttatt tttatttaat tctcgcaaca
      661 gggaaataat tcctccctca aaataatagt gtttttccga gtgatgaatt tcattgaaat
      721 aatgaaaata tattttggga gtaagataag cccgatcttt tagtgatttg ataataattt
      781 tgggatcaaa atcaagagtt tctttgaaga tttctggatc gggataaaaa gttacggtag
      841 taccggtaat ttccttagga attaaatctt taagcaaggc cactttagtt tcgggaattt
      901 ctgtcagttt ggttttaact tcaccccgaa caaattccat ccaatagatt ttgtgctggc
      961 ggcgaatttc gatttgataa agttgtgata gagcgttgac acaggcggca ccgacaccat
     1021 gaagaccacc agaaatttta taggcagtac cgtcaaattt accgccggaa tgcagggttg
     1081 tcataatgac ttcaactgcc ggttttttat atttaggcat aatgtcaatc ggaaa
//
LOCUS       DAOOIE010000170         1130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_107914_length_1130_cov_2.155349, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000170 DAOOIE010000000
VERSION     DAOOIE010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1130
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107914_length_1130_cov_2.155349"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1064)
                     /locus_tag="PK131_03485"
     CDS             complement(<1..1064)
                     /locus_tag="PK131_03485"
                     /inference="COORDINATES: protein motif:HMM:NF015891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HPD45214.1"
                     /translation="MLAPTAEGQGPHSGGIAFVPITRINILMAVSSNDKLLEKAIEWA
                     KKLDIEISEGGAKVFVYFAQNAKAIELANVLNQVFLGLPPQQTTTFQSKLRETKKSTT
                     SSSLLRPRRSLTQPSQTTSQQSDQPPVQTQLRPTLQPSQKESAQSSCLLKGEVQIVVD
                     ETTNALIIQASERDYRVIEKTLKQLDIYPKQVLIEVMIAEIRLGDELKMGVEWEYTKS
                     DIKNGVGLNPAQVTGLADLAAEISSGLKLVIDKTGRFKATIRALADKSKVNILSSPHI
                     IASDNKEAEIDVTQEVPISSGTVTTATDQPVITTTTEYRDTGIILKVTPHINDKGLVT
                     LDISQEVSEIDTATKVEGND"
BASE COUNT          261 a          262 c          260 g          347 t
ORIGIN      
        1 ttatcatttc cctcaacctt tgtcgcggta tctatttcgc tcacttcctg gcttatgtcg
       61 agggtcacca gccccttatc attgatatgc ggggtaacct taagaattat tccggtatcc
      121 cggtattcgg tggtagtggt gatcaccggc tggtcggtag cggtggtaac agtgccggag
      181 gaaatgggga cttcctgagt aacgtcaatc tccgcttctt tattgtcgga ggcgataata
      241 tgtggcgaag ataagatgtt taccttgctc ttatcagcca aagcgcgaat ggttgcttta
      301 aaccggccgg ttttatcaat cactaacttt aaaccagaac tgatttcagc tgcgagatct
      361 gctaatccgg ttacctgggc ggggttaagg cccacgccat ttttaatatc actcttcgtg
      421 tattcccatt ctactcccat ttttaattca tcacccagtc tgatttcggc aatcataacc
      481 tcgattaaca cttgcttggg ataaatatca agttgtttga gagtcttttc gataacacgg
      541 taatcccttt cacttgcctg aataataagc gcattagtcg tttcatctac tacaatctga
      601 acttctcctt taagtaaaca acttgactgc gccgattctt tttgggacgg ttgaagagtt
      661 ggacgcagct gagtttgtac tggcggttgg tcggattgct gagaggtggt ctgactcggc
      721 tgggtaagtg agcgtcgcgg ccggagaagc gatgatgaag tagtggattt tttggtctcc
      781 ctcagcttgc tttgaaacgt ggttgtctgc tggggtggca atccaagaaa aacttgattc
      841 aaaacattag caagctctat tgcttttgcg ttctgagcaa aatagacaaa aacttttgct
      901 cccccttcgc ttatctcaat atcgagcttt tttgcccact cgatcgcttt ttctaaaagc
      961 ttatcattag aactgaccgc catgaggata ttaatccggg tgatcgggac aaaggcgatc
     1021 ccacccgagt gaggcccttg cccttcggca gtaggcgcaa gcactttaaa gatggtatca
     1081 agttcggttg ccatttcctc cacgtcagcc ttttctacct cataaagctg
//
LOCUS       DAOOIE010000171         1129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_107999_length_1129_cov_3.415270, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000171 DAOOIE010000000
VERSION     DAOOIE010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107999_length_1129_cov_3.415270"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PK131_03490"
     CDS             <1..576
                     /locus_tag="PK131_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPD45215.1"
                     /translation="TSGPHVGHFLGLLKNGKEFGLKHLEYAYQEKPDGGIADALSLAE
                     DFADNGPITVILGDNTTDANIAQAVKKFNGGAHLFLRKVTDPRRYGVVQFDQKKKIVK
                     IIEKPKTPPSNLAVTGLYIYDSQVFSFIKGIIPSRRGELEITDVNNMYLQKGHLSWSM
                     LGGYWTDAGTFDALFEANQYWYKKAQKIKKS"
     gene            complement(573..1040)
                     /locus_tag="PK131_03495"
     CDS             complement(573..1040)
                     /locus_tag="PK131_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPD45216.1"
                     /translation="MPQSTNNNRPYDERSAGGLVYKREGNKIYWLVIKTAGREKTKTF
                     YKLSKGHTRNQEYLKQTALREVEEEGQIKAKIVAKLGSNNYFIWDRQQRKRIVKKVTF
                     FLMEYTGESRLRHYDAEMVIDHEWLPLEKAMKKLTFEGERKLLQKALKYLENH"
BASE COUNT          301 a          223 c          217 g          388 t
ORIGIN      
        1 actagtggtc ctcatgttgg tcattttctt ggtttattaa aaaatggaaa agaatttggt
       61 ttaaaacatc tggaatacgc ctatcaagaa aagcctgatg gtggcattgc cgatgcccta
      121 tctttagccg aagactttgc tgataatggt cctataaccg ttattttagg tgataatact
      181 accgacgcta atattgctca ggcggtaaag aaatttaatg gcggggctca tctttttctt
      241 cggaaagtaa ctgatccccg acgatatggg gtagtccaat tcgatcagaa gaaaaaaatc
      301 gttaagatta ttgaaaaacc taaaactccc cctagtaatt tggcagttac cggtttgtat
      361 atttacgaca gccaagtttt ttcttttatt aagggtatta ttccctctcg tcgaggagaa
      421 ttggagatta ccgacgttaa taacatgtat cttcaaaaag gccatttaag ctggtcgatg
      481 ttgggtggct attggaccga tgccggcact tttgatgccc tttttgaagc taaccaatat
      541 tggtataaaa aggcgcaaaa gatcaaaaaa tcttagtgat tctctaggta ttttaaagct
      601 ttttgaagca gttttctttc tccctcaaag gttagttttt tcatcgcctt ttccagcggc
      661 agccattcat gatcgataac catttcggcg tcataatgtc ttaagcggct ttctcctgta
      721 tattccatga gaaaaaaagt tacctttttg acgattcttt tcctttgctg tctgtcccag
      781 ataaaataat tatttgaacc taatttggcg acaattttag ctttgatctg cccctcctct
      841 tctacttcgc gcagagctgt ctgtttcaaa tattcctgat ttcgagtgtg tcctttggat
      901 agtttataga aagttttggt tttttctcgg ccagcagttt tgattaccag ccagtaaatc
      961 ttattacctt cccgcttata aactaatccc cctgctgatc tctcatcata gggccgatta
     1021 ttatttgtcg attgaggcat tttataaatt gaaaattaga aattaatttt tggtgtacct
     1081 agtgggattt ggacccacaa ccttcagctc cggaggctga cgctctatc
//
LOCUS       DAOOIE010000172         1118 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_109746_length_1118_cov_1.976482, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000172 DAOOIE010000000
VERSION     DAOOIE010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_109746_length_1118_cov_1.976482"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..793)
                     /locus_tag="PK131_03500"
     CDS             complement(35..793)
                     /locus_tag="PK131_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45217.1"
                     /translation="MDKNILAELRKKKWYLHSPPAVKNELKDLPLEFHGFLADLAKRK
                     SFIGRVEILRLLKILRGNYLIIPVFEVRSLDTNEVFTYEFVSWKFGRFPGYKGIILVE
                     VKGRIKYFIVKYGAKFPIGADCYDSIGTFYSPDNLKKTITVPNKIKNHLRKFLGLPQV
                     HFKKFIDLGILNPDPGMSNNHIDLFAVVIDDDNAQKIDELLAHNPQKSLVLGYELEIV
                     PVTQLHDFIKRCNDGFFLACIARLFSQKVLEISG"
     gene            833..>1118
                     /locus_tag="PK131_03505"
     CDS             833..>1118
                     /locus_tag="PK131_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45218.1"
                     /translation="MSFPTNRAFKDLVAAAQKSRLNCRDQTNLFCYLGFEGEKARLGW
                     VIPPQWEAVAAKLNDFEKLDLAREIKQVIFVGMGGSINAIKALKTAFAGSG"
BASE COUNT          351 a          244 c          225 g          298 t
ORIGIN      
        1 aatcgaaaac agcaatctaa agggaccggc caaactagcc ggaaatttct aaaacttttt
       61 gagaaaagag gcgggcaata caggccagaa aaaagccgtc attgcatctt ttaataaaat
      121 catgaagttg ggtcacaggc acaatttcta attcatagcc caaaaccaat gatttctgcg
      181 ggttatgagc caataactcg tcaatctttt gggcattgtc atcgtcaatc actaccgcaa
      241 aaaggtcaat atgattatta ctcattcccg gatcgggatt aagaatacct aaatcgataa
      301 atttcttaaa atgaacctgg ggcagcccca aaaacttgcg taaatgattc ttaattttat
      361 tgggaacggt aatggttttt tttagattat cagggctgta gaaagtaccg atggaatcat
      421 agcaatcggc gccgatggga aacttggccc cgtacttgac aataaaatat ttgatcctcc
      481 ctttgacctc cactaaaatt atccctttat atccgggaaa acggccgaat ttccagctga
      541 caaattcata agtaaaaacc tcattggtat ccagagagcg aacctcaaaa acggggataa
      601 tcaaataatt gcctcggaga attttcagca gacggagaat ctccacccga ccgataaagc
      661 ttttcctctt ggctagatca gctaaaaagc catggaattc caagggtaaa tctttcaatt
      721 cattcttaac cgccggcggg gaatgaagat accatttttt ctttcttaat tcggctagaa
      781 tatttttatc catagagtaa gaatagcaca tcttatgtta taatcaccaa ctatgagttt
      841 tccgactaat cgggctttta aagacctggt agcggcggct caaaagtcac gccttaattg
      901 ccgggatcaa actaaccttt tctgttacct cggatttgaa ggcgaaaaag ccagactagg
      961 ctgggtcatt cccccccaat gggaagctgt tgccgccaaa ttgaacgact ttgaaaaact
     1021 agatctagct agggaaatta agcaggttat tttcgtcggc atgggcggtt cgatcaacgc
     1081 catcaaagcc ctgaaaaccg cctttgctgg ctcgggca
//
LOCUS       DAOOIE010000173         1103 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_112220_length_1103_cov_2.400763, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000173 DAOOIE010000000
VERSION     DAOOIE010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_112220_length_1103_cov_2.400763"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1103
                     /locus_tag="PK131_03510"
     CDS             <1..>1103
                     /locus_tag="PK131_03510"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HPD45219.1"
                     /translation="NYRSQQMILDSSRYVIEKSVNRITKILSGIDKSLIAMVNDQPHP
                     INLLAAASDYEENYFVANKIKTLIKDGISPNEIAVLYHDNADVTGLLPWLDNLGIKYR
                     LSSGNNILNDLIINQFISLLKYIDDPSRDDQLALILNFDFLKIDPLALVNYFRFKKTT
                     DKKITRIIHLLGKAHQWRFNLSPEKFFHRVIRRFGLLKSVIQEKDYLTLLKFNRFYEE
                     FRRFVNERQGSLTDFLHYLDLLKDNKVALDIGDTLPDDNSINLMTVHKAKGLEFDQVF
                     LIHLTDRRWGGGYERSKIKLPYGIIKYEVVRPFNSINEDDRRLFYVALTRAKRDLYLS
                     YTKENPTAFIAEIKPELINNIKPEVSFKQEALLS"
BASE COUNT          343 a          178 c          193 g          389 t
ORIGIN      
        1 aattatcgct cccaacaaat gattttagat agttctcgtt atgttattga aaagagtgtt
       61 aatcgaatta ctaaaatttt aagcggcatt gataaatcgt taatagctat ggttaatgat
      121 caacctcacc caattaatct tttagccgct gcctctgatt atgaagaaaa ttattttgtc
      181 gccaataaaa ttaaaactct gattaaggac ggtatatctc ccaatgaaat tgccgttctc
      241 taccatgata atgctgatgt taccggatta cttccttggc tggataatct tggcattaaa
      301 tatcgcctat cttcaggtaa taatatttta aatgatttaa taattaatca gtttatttcc
      361 cttttaaagt atattgatga cccaagtcgg gatgatcaac tggcattgat tcttaatttt
      421 gattttttaa aaattgatcc cctagctttg gttaattatt ttcgttttaa aaaaactact
      481 gacaaaaaaa ttaccagaat tattcacctt ttgggtaaag cccatcaatg gcggtttaat
      541 ttaagtccag aaaaattttt ccatagggtt attcgccgtt tcggtttgct caaatccgtt
      601 attcaggaga aagattatct gactttgtta aaattcaatc gtttttatga ggaatttaga
      661 cgatttgtta atgaaagaca gggaagttta actgattttc ttcattatct cgatctttta
      721 aaagataata aagtggctct tgatattggt gataccttac ctgatgataa ttcaattaat
      781 ttaatgacgg ttcataaagc caagggtttg gaatttgatc aggtttttct aattcatttg
      841 accgatcgcc gctggggcgg tggttacgag aggtctaaaa taaaattacc ctatggtatc
      901 ataaaatatg aggtagttcg gccatttaac tcgattaacg aagatgatcg gcgtttgttt
      961 tatgtagctt tgaccagggc gaaacgggat ttatatttgt cttacaccaa ggagaacccc
     1021 accgccttta ttgccgaaat taagccggaa ttgattaata atattaaacc tgaagtcagt
     1081 tttaaacaag aagctcttct gtc
//
LOCUS       DAOOIE010000174         1096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_113420_length_1096_cov_1.794428, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000174 DAOOIE010000000
VERSION     DAOOIE010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113420_length_1096_cov_1.794428"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..841
                     /gene="prfB"
                     /locus_tag="PK131_03515"
     CDS             <1..841
                     /gene="prfB"
                     /locus_tag="PK131_03515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPD45220.1"
                     /translation="RPMVEGQLEKLKERLDQQEIKRFLSGPYDHGGAIIEITAGAGGR
                     EAEDWVAMLARMYRRYADRQDWSSVILDQSFGESGGPDGRIGLKAVVIEIKGKNVFGY
                     LKNEQGVHRLVRISPFSDQQLRHTSFALVEVLPILGKNESDLEINPGDLRIDTYRASG
                     PGGQYVNKTESAVRITHLPTKIQVACQAGRSQGANREMAMNLLKAKLKKRWEEEEAQK
                     ISALKSGIKSAAWGQQIRSYVIQPYQLVKDYRTEVETSNVEAVLDGQLDDFIKAEIKL
                     NND"
     gene            834..>1096
                     /locus_tag="PK131_03520"
     CDS             834..>1096
                     /locus_tag="PK131_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPD45221.1"
                     /translation="MIKFDQVSKLYPNSCLALDNINLEIKQGEFVTIVGKSGAGKTTL
                     IKLLIREELPTEGQVYFDGQSVLEMNRRQICQLRQRIGVVYQD"
BASE COUNT          289 a          235 c          314 g          258 t
ORIGIN      
        1 tcggccgatg gtcgagggac aattggaaaa gcttaaggag aggcttgacc agcaggagat
       61 caagcgtttc ttgagcggcc cgtacgacca cggcggcgca attatcgaaa ttactgccgg
      121 cgccggcggc cgggaggccg aggactgggt ggcgatgctg gctagaatgt atcggcgcta
      181 cgctgatcga caggactgga gctcggttat tcttgaccag tcctttggtg aatccggcgg
      241 gccggatggg cggattggat taaaagcagt agttattgag ataaaaggca agaatgtttt
      301 tggctatctc aagaacgagc agggggttca tcgcttggtt agaatttctc ctttttctga
      361 tcagcagctg cgccacactt cttttgccct ggttgaggtg ctgcccattc ttggaaagaa
      421 cgagtctgat ctggagatta atccgggcga tcttaggatc gacacttatc gggcttcagg
      481 accgggtggc cagtacgtta acaagaccga gtcagcggtg agaattaccc atttgccaac
      541 caaaattcaa gttgcttgcc aagccggacg gtcacagggg gccaaccgcg agatggccat
      601 gaatctgtta aaggctaaat taaaaaagcg ctgggaagaa gaagaggctc aaaagatctc
      661 ggctcttaag agcggcatca aaagcgctgc ctggggccaa cagattcgct cttatgttat
      721 acagccctat caattggtta aagattaccg aaccgaggtc gagacgtcaa atgtcgaggc
      781 tgtgctagat ggtcagctag acgatttcat taaagctgaa attaaattga acaatgatta
      841 agtttgacca agtgagcaaa ctttatccta actcctgcct ggctctcgat aatattaacc
      901 tggagattaa gcagggcgag tttgtgacta ttgtcggtaa atcgggagcc ggcaagacga
      961 ccttaattaa gctcttgatc cgggaagagc tgccaaccga gggccaggtt tattttgacg
     1021 gacaatcggt attagagatg aatcgtcgcc agatctgcca gctaaggcag cggatagggg
     1081 tggtctatca agatta
//
LOCUS       DAOOIE010000175         1093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_113872_length_1093_cov_2.368015, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000175 DAOOIE010000000
VERSION     DAOOIE010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113872_length_1093_cov_2.368015"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="PK131_03525"
     CDS             <1..721
                     /locus_tag="PK131_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HPD45222.1"
                     /translation="RCFTIERRLQMELDIPVMHDDQHGTAAVVLAALINALKLRGSDR
                     SDIKVVISGAGAAGNAIARLLFGYGVKNIIACDRQGAIYVGRPDLDKEKADLATFTNL
                     GKQKGALAEVLVGADVFIGVSSAGLLTREMVRTMAPKSLVFALANPVPEIMPDEAKAA
                     GAFIVASGRSDFPNQINNVLIFPGVFRGALDNKVRQISDERLVKAAENLAALVSVPRV
                     DRILPEPFTPGIAAAVAAAIK"
     gene            727..>1093
                     /locus_tag="PK131_03530"
     CDS             727..>1093
                     /locus_tag="PK131_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45223.1"
                     /translation="MKKIFFFLIIIAAAIFFLMDKGRPGSENNESVPVTAEVLPTASI
                     GLPEREAASLDEKEKRENVLEADIKIKAAAPLASSHALINGNENIILSVPFLSQAPLA
                     NWEDARQQDGCEEAGALMAM"
BASE COUNT          253 a          242 c          304 g          294 t
ORIGIN      
        1 ccgctgtttt accattgagc gccgtttgca gatggaactc gatattccgg tgatgcacga
       61 cgaccagcac ggcacagcgg cggtagtttt agcggcctta atcaatgctt tgaagttaag
      121 aggtagtgat cggtcggata ttaaagttgt tatcagtggc gctggcgctg ccggcaatgc
      181 gattgcccgt cttttgtttg gttatggtgt aaaaaatatt atcgcttgtg accgtcaagg
      241 cgcgatttat gtcggtcgtc ccgatttaga caaggaaaaa gccgatttag ccaccttcac
      301 caatctgggc aaacaaaaag gcgcgctggc tgaggttttg gttggagcgg atgtttttat
      361 cggcgtttct tcagccggtt tgcttacccg ggaaatggtc aggacaatgg cgccaaaatc
      421 attggtgttt gccttggcta atccggtgcc ggaaattatg ccggatgaag ccaaagctgc
      481 cggcgctttt attgtggcca gcggccgcag tgattttcct aatcagatta ataacgtgct
      541 gatttttcct ggcgtctttc gcggcgcttt ggataataag gtgcgccaga ttagcgatga
      601 gaggctggta aaggcggcgg agaatttggc cgctttagtc tccgttcccc gggttgacag
      661 aattttaccg gagccattta ctcccggtat cgccgcggcg gtggcggcgg cgattaaata
      721 aagacgatga aaaagatttt tttctttttg ataatcatcg cggcggcaat ttttttccta
      781 atggacaaag gccggccggg aagtgaaaac aacgagtctg ttccggtaac agccgaggtt
      841 ttgccgacgg ccagtattgg cttgccggaa cgagaagccg cttctttgga tgaaaaggaa
      901 aaaagagaaa atgttttaga agctgatatt aaaattaaag ccgccgcgcc cctcgcctct
      961 tcccacgctt tgattaacgg caatgaaaat attattttgt ccgtcccttt cctttctcaa
     1021 gcccctttag ccaattggga ggacgcgcgc cagcaggatg gttgcgaaga agccggcgct
     1081 ttgatggcaa tgg
//
LOCUS       DAOOIE010000176         1090 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_114342_length_1090_cov_2.317874, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000176 DAOOIE010000000
VERSION     DAOOIE010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_114342_length_1090_cov_2.317874"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PK131_03535"
     CDS             complement(<1..480)
                     /locus_tag="PK131_03535"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HPD45224.1"
                     /translation="MKKTTELFLSFLKIGAFTIGGGYAMIPLMQQEIVEKKKWLKPEE
                     VIDMVAISESTPGVLAVNFATYVGYKVAGLWGSIVATLSVVLPAFLIILLLSTVLAAY
                     QDSAVVQAVLKGIKAGVVVLLMRAIVKLVKICPLTPFNLVMILATVLVSLLVKGIVFY
                     "
     gene            complement(577..>1090)
                     /locus_tag="PK131_03540"
     CDS             complement(577..>1090)
                     /locus_tag="PK131_03540"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HPD45225.1"
                     /translation="TLGVLIILGIEWLAGGKRDEAVSIFWLISGGVLTGIVIVLPGVS
                     ASLTLMALNLFFPLVKMAEEFLKSSLAFSFPSVKTVFSLGTFGLSLGIGTILFARIMK
                     GIIKRHPEELTVFSLGLISGSIGSVIAKLPLDGQIWAVPLEIGLGVGLGIFTLFLLNR
                     LIKRPPKHLG"
BASE COUNT          363 a          268 c          202 g          257 t
ORIGIN      
        1 ataaaaaaca atacctttaa ccaacaagga aaccaaaacg gttgccagaa tcatcaccag
       61 attaaacggg gttaaagggc aaatcttgac taatttgaca atggcccgca tcaacaaaac
      121 aaccactccg gccttgattc ctttgaggac agcctgaaca acagcgctgt cttgataagc
      181 ggccaaaacg gtcgacaata aaaggattat taaaaaagcg ggcaaaacga cacttaaagt
      241 agcgacaatg cttccccaaa gccctgccac tttatagcca acgtaggtgg caaaattaac
      301 cgctaagaca cccggagtag attccgagat cgccaccatg tcaattactt cttcgggctt
      361 tagccacttt ttcttttcga cgatttcctg ttgcatcagg ggaatcatcg catagccgcc
      421 gccgatggta aaggctccga tttttaaaaa actgagaaag agttccgtgg tttttttcat
      481 gtttttacct cgcagaaata atattgaccg gcttaagtaa tattattcta tcataatttc
      541 tcccaaatgg caatttatgt gaaaaacatt taggtgttac cctaaatgtt tcggtggccg
      601 tttgattaag cgattaagta aaaaaagcgt aaaaatcccg agtcctactc ccaaaccgat
      661 ttccaaggga accgcccaaa tttgcccgtc aagcggcagt ttggcaatca ccgaaccaat
      721 cgaaccgctg attaagccaa gagaaaaaac ggttaattct tccggatggc gtttgataat
      781 tcctttcatg attcgggcaa aaaggattgt acctatccct aaacttaacc caaaggtgcc
      841 aagggaaaaa accgtcttta ccgaaggaaa ggaaaaagcc aaagaagact ttaagaattc
      901 ttccgccatc ttaaccaaag gaaagaacag gttcaaagcc attaaagtca aactggcgct
      961 gactcccggc aatacaatca caattccggt taaaaccccg cccgaaatca gccaaaaaat
     1021 acttaccgct tcatcccttt tcccgcccgc caaccattcg atgccaagga taatcagcac
     1081 tcccaaagta
//
LOCUS       DAOOIE010000177         1080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_116060_length_1080_cov_2.108293, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000177 DAOOIE010000000
VERSION     DAOOIE010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116060_length_1080_cov_2.108293"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="PK131_03545"
     CDS             complement(<1..612)
                     /locus_tag="PK131_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45226.1"
                     /translation="MKISNKVTLVILITILGLFYPCQILAKEYKTSIEKKVYAHIVDM
                     LGSNNDEQANNYYQIILDKIEFGEDFSDWPSADYFLKNSSLFYDNPNNQTKRALDQRY
                     QNLSSKTNQSSLSEESKIKNRIQKLIATMPGNNHDDQTNAYYETILDKKERQEAFNDL
                     EAADYFLQNSIFFYFNPNDPVKLTLDKRYERLSSGNSSSQPVLS"
     gene            671..>1080
                     /locus_tag="PK131_03550"
     CDS             671..>1080
                     /locus_tag="PK131_03550"
                     /inference="COORDINATES: protein motif:HMM:NF018435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPD45227.1"
                     /translation="MKLLRRLGIDLGTSNTLIWAENEGVVLSEPTLVAVSIEERKILA
                     VGNEAKEMVGKTPEYIEVVKPLEDGAIADYEVSEAMIKAFLRRVIGKNWFVGPEVMIG
                     VPAGKTQVEQRAVLDAALGAGARKVYLIDKPLAAA"
BASE COUNT          337 a          150 c          238 g          355 t
ORIGIN      
        1 gcttaaaact ggttgggatg acgaattacc cgaggataat ctttcatatc ttttatccaa
       61 ggttaactta acgggatcat taggattaaa gtagaaaaag atagaatttt gaagaaaata
      121 atcagcggct tccagatcat taaaggcttc ctgtctttct tttttatcta aaatagtttc
      181 atagtaagca tttgtttggt catcatgatt gttgccaggc atggtggcaa ttaatttttg
      241 gattctattt ttgattttac tttcttcgga gagagaagat tgatttgttt ttgaactaag
      301 gttttgatac ctttgatcca aagctctttt ggtttggtta ttgggattat cgtaaaaaag
      361 agaagaattc tttagaaagt aatcggctga cggccagtca ctgaaatcct ctccaaactc
      421 aattttatcg aggataattt ggtaataatt atttgcctgc tcatcattat tacttcctaa
      481 catatcaaca atatgggcat ataccttttt ttcgatacta gttttatatt ccttagccaa
      541 aatttggcat ggataaaaaa gtcccagtat tgtgattaaa ataactagag ttactttatt
      601 actaattttc atagttgcta gtataacata tattttataa gatgcaatag gtaatatgct
      661 agaatgaaat atgaagttgt taagaaggtt agggattgat ttggggacat ccaatacttt
      721 aatttgggca gaaaatgagg gggtagtttt gtcagaacca accctagtag cagttagtat
      781 tgaggaaaga aagattttag cagtcggtaa tgaggccaag gagatggtgg gtaaaacccc
      841 ggagtatata gaagtagtta aacctttaga agatggggct attgctgatt atgaagtaag
      901 tgaagcaatg attaaagcgt ttttacgccg ggtaattggt aaaaattggt ttgtcgggcc
      961 ggaggtgatg ataggtgttc cagctggcaa aactcaggta gaacaaagag cagttctaga
     1021 tgcggctctg ggagccggcg cccggaaagt atatttaatt gataaaccgt tagcagccgc
//
LOCUS       DAOOIE010000178         1072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_117322_length_1072_cov_3.364798, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000178 DAOOIE010000000
VERSION     DAOOIE010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117322_length_1072_cov_3.364798"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1050)
                     /gene="argS"
                     /locus_tag="PK131_03555"
     CDS             complement(<1..1050)
                     /gene="argS"
                     /locus_tag="PK131_03555"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPD45228.1"
                     /translation="MVDKIEIIGHYVNVYYKAAYLVREAEKINYEIEFKNELNQLNNG
                     KTVVIDYSSPNIAKTFGIGHLRSTNIGQALYNLYQILGWNCIGENHLGDYGTQFGKLI
                     TAIKKWGPINWSTAKVDDLESLYIKFHRNETEELLNEARNWFAKLENKDPEAMAIWQK
                     CVDLSQQEFNQIYDLLDVHFDYCHGESFYRPMTDEIIQIMKDKGLTKESQGATIIEFE
                     DHSVAIVKKSNETSTYFDRDIAALKYRRDTWHPDLIIYEVGADQTLYFKQVFTTVEKL
                     GWFKPANLHFVGHGLIRQQQGKFSTRKGQTIHLKDIITKTIALAKKIAPDNSAEAVRA
                     IAIGAIKFNDLAQNPR"
BASE COUNT          305 a          198 c          200 g          369 t
ORIGIN      
        1 tcgagggttc tgggccagat cgttaaattt aatggcccca atggcaatgg ctctaactgc
       61 ttcggcgcta ttgtcgggag caattttttt agccagagct atcgtttttg taataatatc
      121 ttttaaatga atcgtttggc ctttccgagt agaaaatttc ccttgctgtt gacggattaa
      181 accgtgaccg acaaagtgaa ggttggcggg tttaaaccaa ccaagttttt ctactgtagt
      241 aaaaacctgt ttgaaataga gggtttgatc agcgccgacc tcgtaaataa ttagatcagg
      301 atgccaagta tcacggcgat attttagagc ggcaatatct cggtcaaaat aagttgaagt
      361 ttcgttagat tttttgacta tagctacgga atgatcctca aattcaataa tagtcgctcc
      421 ttgtgattct ttagttaaac ctttatcttt cattatttga ataatttcat ctgtcattgg
      481 ccggtaaaaa gactcaccgt gacaatagtc gaaatgaaca tctaagagat cataaatttg
      541 attaaattcc tgttgggata gatcaacaca tttttgccaa atggccattg cttccggatc
      601 tttattttcc agtttggcga accaatttcg ggcttcatta agcagttctt cagtttcatt
      661 tcgatgaaat ttaatataga gactttctaa atcatcgact ttggcagtcg accaattaat
      721 tggcccccat tttttaatag ccgtaattaa tttgccaaat tgggtgccgt aatcacctaa
      781 atgattttcg ccgatacaat tccagcctaa aatctggtaa agattataca gggcttggcc
      841 gatgttagtt gagcgtaagt gaccgatgcc aaaagttttg gcaatattag gtgaactata
      901 atcgataact acggttttac cgttgttaag ctggtttagt tcatttttaa attcgatttc
      961 ataattgatt ttttctgctt ctctaactaa ataagccgct ttataataaa cattgacata
     1021 gtgaccaata atttcgatct tatccaccat atctcggtct aacttttttt cg
//
LOCUS       DAOOIE010000179         1072 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_117405_length_1072_cov_1.947886, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000179 DAOOIE010000000
VERSION     DAOOIE010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_117405_length_1072_cov_1.947886"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1072
                     /locus_tag="PK131_03560"
     CDS             <1..>1072
                     /locus_tag="PK131_03560"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HPD45229.1"
                     /translation="KGKIPIYEPGLADLVTKNIKEKRLEFTINLAEAVKKSEIIFIAV
                     GTPSLPDGTVNLDYVKTVAESIGKVMNGPKIIVNKSTVPIGTGNMVARIIKQFYSGDF
                     EVVSNPEFLREGSAVSDFMKPERIVIGNGKQKVKKIMIDLYKPLKAPMVITDIKTAEM
                     IKYASNALLATEISFINSVANICEKLGADVTKVAEGMKLDRRIGQYAFLEAGVGYGGS
                     CFPKDVKALIQIAHENGERFSILEAVEATNEAQKQSLLVKIQSLVGNDLATKRITLWG
                     LAFKPQTDDLREAPSLVIIKQLLDRGAKVVAFDPIAREQAQKILGKAIDFETDCYQAA
                     RRADCLVVVTEWPEFKQVDLKKL"
BASE COUNT          348 a          161 c          248 g          315 t
ORIGIN      
        1 caagggtaaa attcctattt acgaaccagg attggcggat ttagtgacca aaaatatcaa
       61 agaaaaaaga ttagagttta ctattaatct ggctgaagcg gtaaaaaaat cagagataat
      121 ctttatcgct gtcggtaccc cttccctacc agatggcacg gttaatttgg attacgttaa
      181 aacggttgca gagagtattg gtaaagtaat gaatggtccc aagattattg taaataaatc
      241 aacggtgccg attggcactg gcaatatggt tgccaggatt attaaacagt tttattcagg
      301 tgattttgaa gtcgtttcta atcctgaatt tttaagggaa ggatcggcag ttagtgattt
      361 tatgaagcca gaaagaatcg ttattggcaa tggaaaacag aaagtgaaaa agattatgat
      421 tgatctttat aagccattga aggcgccgat ggtgattact gatattaaaa cagccgagat
      481 gatcaagtat gcttctaatg ctcttttagc cacagaaatt tcctttatta attcagttgc
      541 taatatttgt gagaaattag gtgctgatgt gactaaagtg gcagagggaa tgaaattaga
      601 cagaagaatt ggtcaatacg cttttttaga agcgggcgtt ggttatggcg gcagttgttt
      661 ccctaaagac gttaaagccc tgatccaaat tgctcatgaa aacggtgaaa gatttagtat
      721 tttggaagct gtcgaagcga caaatgaagc tcaaaaacaa tcacttttag ttaaaattca
      781 gtctttggtg ggtaatgatt tggcaactaa aagaattacc ctttggggat tagcctttaa
      841 acctcaaaca gatgatctcc gggaagcacc atctttagtt attattaagc aattattaga
      901 ccgaggggct aaagtagtcg cttttgaccc cattgcccgg gaacaagccc aaaaaatttt
      961 aggcaaagca attgactttg agactgattg ttatcaggcg gctagaaggg cggattgttt
     1021 agtggtggtt acagaatggc cggaatttaa gcaggttgat ttgaagaaat ta
//
LOCUS       DAOOIE010000180         1066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_118485_length_1066_cov_2.229476, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000180 DAOOIE010000000
VERSION     DAOOIE010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118485_length_1066_cov_2.229476"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1066
                     /locus_tag="PK131_03565"
     CDS             <1..>1066
                     /locus_tag="PK131_03565"
                     /inference="COORDINATES: protein motif:HMM:NF014874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPD45230.1"
                     /translation="PGRFFLQRTLRRMVNNGCQAAIIETTSQGIEQFRHRFINYDLLV
                     FTNLYPEHIDAHGSFENYKRAKGKLFSHLCHCRSKYLDDNNQVVSHPSGLKQTELLKV
                     KKTIIVNGDDDQASYFLSFPAERKCVYTLNPNLVATSLTDDSGQEIQPPPEIFLYEPL
                     FATASGSDFQFNGQRCHLSILGSYHVFNAVAALTVASVLNIKTEQAAAALANIKSLAG
                     KMEKIEAGQNFHVLIDYAFEPKALEALYQNLNFIHYRRLIHILGSAGGGRDQARRSIL
                     GKMAGEKADIVIVTNEDPYDDNPQDIINQVAAGAEKAGKILESNLYKILDRREAMRLA
                     FRLAGEGDLVLITGKGAEQY"
BASE COUNT          286 a          216 c          238 g          326 t
ORIGIN      
        1 tgcccggtcg tttttttcta caacggactc ttcggcgaat ggttaataac ggttgtcaag
       61 ccgctattat agaaaccact tctcaaggga tagagcagtt ccgtcatcgt tttattaact
      121 atgatttatt agtttttacc aatctctatc cggaacatat tgatgcccat ggcagttttg
      181 agaactacaa acgtgctaag gggaagctat tttctcatct ttgtcattgc cgctctaaat
      241 atttagatga taataatcag gtagtttctc acccgagcgg attgaaacag accgaacttt
      301 taaaggttaa aaaaacaatt attgtaaatg gcgacgatga tcaggcttca tattttttat
      361 ctttcccggc cgaacgtaaa tgcgtatata cgcttaatcc gaatttggtg gcgacaagcc
      421 taacggatga ctctgggcag gagatccaac cgccaccaga aatttttctt tatgagcctt
      481 tattcgccac cgcctcaggt tctgattttc aatttaacgg ccagcgctgt catctttcga
      541 ttctcgggtc ttaccatgtt tttaatgccg ttgccgcttt aacagtcgct tctgtgctta
      601 atattaaaac tgaacaagcg gcggccgctt tggcaaatat taaatcttta gccggcaaga
      661 tggagaagat agaggccggc caaaattttc atgttttgat tgattatgcc tttgagccta
      721 aagccttaga ggccttgtat caaaacctta attttattca ttatcgccgc ttaattcata
      781 ttttaggttc ggctggaggc ggtcgtgatc aggctcgtcg gtcaatttta ggtaaaatgg
      841 ctggagaaaa agcggatata gtgattgtga ccaatgaaga tccatatgac gataatcctc
      901 aggatattat caatcaagtg gcggcggggg cagaaaaggc agggaaaatt ctagaatcga
      961 atctttataa aattcttgat cggcgtgaag ctatgcgcct ggctttccgt ctggccggcg
     1021 aaggcgatct cgttctgatt accggtaaag gggctgagca gtatat
//
LOCUS       DAOOIE010000181         1057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_120104_length_1057_cov_3.686627, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000181 DAOOIE010000000
VERSION     DAOOIE010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120104_length_1057_cov_3.686627"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..711
                     /gene="lexA"
                     /locus_tag="PK131_03570"
     CDS             94..711
                     /gene="lexA"
                     /locus_tag="PK131_03570"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HPD45231.1"
                     /translation="MTAVLYKRQKQILDFIERQIDKYGCAPTLTEIADHLGLSSLATV
                     HEHLSVLEKKGFIRRYQGAVRGIELLKGTKEKVRNLVELPILGFIACGAPLEPYTDPN
                     ATLLISSNLICPGEKSFILQAKGDSMVDDGILNGDYLVVKEQHQANNGDIVVAVLKNG
                     FATLKRFYQEANLIRLQPANVNMSPIYVKDVEIKGKVVAVIRQFN"
     gene            713..>1057
                     /locus_tag="PK131_03575"
     CDS             713..>1057
                     /locus_tag="PK131_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPD45232.1"
                     /translation="MLYLITTPIGNLNDISQRAIEVLEKVDILLCEDTRSNLLDLLGI
                     KNKPIRESFYDEVEERKIPQIIKWLREGKIIGLITKAGTPLISDPGWRLVKQCQKLGL
                     KYTAIPGPCAAIN"
BASE COUNT          343 a          163 c          223 g          328 t
ORIGIN      
        1 ccagccaaac aaaacaagaa taaatagcat atcttattta tagtttacat gatccagttg
       61 actttgggta atgttcgggt atgataagaa gtaatgactg cagttttata taaacgacaa
      121 aaacaaattc tagattttat cgagcggcag attgataaat atggctgtgc cccaaccttg
      181 acggaaattg ccgatcattt gggtttatca tcactggcta ctgtccatga acatttatcg
      241 gttttagaaa aaaaaggttt tattcgacgt tatcaaggag cggttagagg gattgaatta
      301 ttaaaaggga ccaaggaaaa ggtcaggaat ttggttgaat taccaatttt aggttttatt
      361 gcctgtggag ctccactaga gccatacact gatcccaatg ccaccttatt aattagttct
      421 aatttaattt gccctgggga aaaatctttt attttacagg ccaaaggcga ttcaatggta
      481 gatgacggta ttttaaatgg tgactattta gtggttaaag aacaacatca agcgaataat
      541 ggtgatattg tggttgctgt tttaaagaat ggttttgcta ctcttaaaag attttatcag
      601 gaagctaatt taattcgact ccaaccagct aatgttaata tgtcccctat ttatgttaaa
      661 gatgtagaga ttaagggcaa agtagtggcg gttatcaggc aatttaatta ggatgttgta
      721 tctaattact accccaattg gcaatctaaa tgatattagt caacgggcaa ttgaagtttt
      781 ggaaaaagtc gatattttac tctgtgaaga taccagaagt aaccttttag atttattggg
      841 aataaaaaat aaaccaattc gggaatcatt ttacgacgaa gtggaagagc gaaaaatacc
      901 gcagattatt aaatggcttc gggagggaaa aattattggt ttgatcacca aagccgggac
      961 gccgcttatt tctgatccgg gttggcggct ggttaaacaa tgtcaaaaat tagggttgaa
     1021 atatactgct attcccggtc cttgtgccgc catcaat
//
LOCUS       DAOOIE010000182         1037 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_124062_length_1037_cov_2.806517, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000182 DAOOIE010000000
VERSION     DAOOIE010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124062_length_1037_cov_2.806517"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1037
                     /locus_tag="PK131_03580"
     CDS             <1..>1037
                     /locus_tag="PK131_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HPD45233.1"
                     /translation="GIPTANGSVHYHPDFRAKPTVIVGAYGLIPEKRAKKGRARFGDL
                     VIVIGGKTGRDGIHGATFSSGVMTQRTIKVNSAAVQIGNAIEEKRMLDAVLEARDKNL
                     IRAITDCGAAGFSSAIGEMGEDIGVTVDISQAPLKYPGLAPWEIWLSESQERMIVAIS
                     PNNKEEFLAVCRKYNVEATVLGRFDGSKKLNVFYGQQKVADLDYDFLKNGLPQRVMTA
                     APLPSAYPPADKVKIRSTPWRREVWGSIIKKVLSHGNVCSKEPIVRLYDHTVQGTNSL
                     PPFTGVYHDGPNDALILTPILGRKYGLIQSHGLNPVLNKIDPYWGSVWAFTEAMANLT
                     AVGGNPRETAL"
BASE COUNT          271 a          219 c          249 g          298 t
ORIGIN      
        1 gggattccca ctgccaatgg ctccgttcat tatcatcctg actttagggc taaaccgaca
       61 gtgattgtcg gtgcttatgg tcttattcct gaaaagaggg caaaaaaggg cagagctaga
      121 tttggcgatt tggttatagt cattggcggt aaaactggcc gtgacggcat ccatggggca
      181 actttttcct ccggagtgat gactcaacgg acgattaagg ttaattcagc cgccgtccaa
      241 attggtaatg ccattgaaga aaaacggatg ctcgacgccg ttttagaagc tcgggataag
      301 aatttgattc gggcgattac tgattgcggc gccgccggtt tttcttcagc tattggcgaa
      361 atgggtgagg atattggggt aacagtggat atcagccaag cccctttaaa atatcctggt
      421 ttagccccat gggaaatctg gttatctgaa tcacaagaga gaatgattgt tgcaatttct
      481 cccaataaca aggaagaatt tttagccgtt tgtcgaaaat ataatgttga ggcgacggtt
      541 ttgggccgtt ttgacggttc taaaaaattg aatgtttttt acggtcaaca aaaagtggct
      601 gatcttgatt atgattttct caaaaatggt ctacctcaac gagtaatgac ggccgcgcct
      661 ctaccctccg cctacccgcc ggctgacaag gtcaaaataa gatccacccc ttggcggaga
      721 gaagtttggg gttcaattat taaaaaagtc ctttctcacg gcaatgtctg ttctaaagaa
      781 ccgatcgttc ggctttatga ccataccgtt caggggacca atagcttgcc accctttacc
      841 ggagtttatc atgatggccc taatgatgct ttaattttaa ccccaatcct tggtcggaaa
      901 tatggtttaa tccagtctca tggtctcaat ccggtcttaa ataaaattga tccttattgg
      961 ggcagtgttt gggcttttac tgaagcgatg gctaatttaa cggcagtcgg cggtaatccc
     1021 cgagaaactg cccttat
//
LOCUS       DAOOIE010000183         1034 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_124607_length_1034_cov_4.163432, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000183 DAOOIE010000000
VERSION     DAOOIE010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1034
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_124607_length_1034_cov_4.163432"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="PK131_03585"
     CDS             complement(<1..360)
                     /locus_tag="PK131_03585"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HPD45234.1"
                     /translation="MKKIVKLILTILLVLGMPAKAAIAAPRLTLSPVSGSYNKGTEFT
                     VKIGAQSEGKKISGVDVVLNYDATKLDVIGNPTLESGVNFIIYPKVESGKITVTLVKD
                     KATDGLESTAVAGDLFTI"
     gene            complement(366..>1034)
                     /locus_tag="PK131_03590"
     CDS             complement(366..>1034)
                     /locus_tag="PK131_03590"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HPD45235.1"
                     /translation="YRFQKVWRLVLGGKMKTDQKINKKQVLNWVIPVFAVIVIFQAVI
                     LVTNSLTPAKTATQVASSTATKKTEAITPVTTELNLSLVSPEEEWQVGKTTTVTLKVT
                     ANTRKAVDAMDLYIKYDPAAFKVTDLTVGEGLPKPASSKISPKTGLIVANIYISGTSS
                     FNLEKAVDKDLITFKVTPQRAGDFNFEIDTGKTARDSVTMLVEAKTAKVIPFTAETFK
                     IKVK"
BASE COUNT          302 a          230 c          168 g          334 t
ORIGIN      
        1 aatagtaaac aaatccccgg ctacagcagt tgattctaaa ccatctgtag ctttatcctt
       61 tactaaagta actgttatct ttccactttc gaccttagga tagataataa aattaacccc
      121 actttctaaa gttgggttgc caataacgtc caatttagtt gcgtcataat ttaaaactac
      181 atcaacacca ctaatcttct tcccttccga ttgagcgccg attttaacgg taaattcagt
      241 tcccttatta taagaaccgc tgaccggtga taaggtcagt ctcggcgccg ctatcgctgc
      301 tttagcgggc atccccaaca ccaagaggat agttaaaatt agtttaacta tttttttcat
      361 taatattatt ttaccttaat tttaaaagtt tcggcagtga aaggaattac cttagccgtc
      421 ttagcctcta ctaacatagt aacagaatcc ctggcagttt ttccagtatc aatttcaaaa
      481 ttaaaatccc ccgctctctg gggagtaact ttaaaagtaa tcaaatcctt atcaactgct
      541 ttttctaaat taaaactgct ggtaccagaa atataaatat tagcgactat taaaccggtt
      601 ttagggctta ttttactcga agccggctta ggcaaaccct caccgacggt cagatcagtc
      661 accttaaagg cagctgggtc atacttaata tataaatcca tagcatcaac agcttttctt
      721 gtattggccg ttactttgag ggtaaccgta gttgttttac ccacctgcca ttcttcttcc
      781 ggcgaaacca gagataaatt taactccgtg gttaccggcg taatcgcctc tgttttttta
      841 gtcgctgttg atgaggccac ctgcgtcgca gttttagctg gcgtcaagct gttggtgact
      901 aaaataaccg cttgaaaaat aacaataact gcaaaaactg gaataaccca atttaaaact
      961 tgttttttat tgattttttg atcagttttc attttacctc ctaaaaccag tctccacact
     1021 ttttggaaac ggta
//
LOCUS       DAOOIE010000184         1024 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_126747_length_1024_cov_1.273478, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000184 DAOOIE010000000
VERSION     DAOOIE010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126747_length_1024_cov_1.273478"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            141..515
                     /locus_tag="PK131_03595"
     CDS             141..515
                     /locus_tag="PK131_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD45236.1"
                     /translation="MKKILIIEDNEEIMGLLSQKISDVGYQVIEARDGEEGLRLLRSN
                     QPDLALLDIILPNIDGLELLKRKQALPETKDIPVVVISNSGAPAEISQAQRLGAVDWI
                     TKTEFSLQETIDKIEKIIKESR"
     gene            527..919
                     /locus_tag="PK131_03600"
     CDS             527..919
                     /locus_tag="PK131_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPD45237.1"
                     /translation="MPNSPKKILIIEDDVFLNELMAKKLVEEGFDVIKAVNGEEGLEM
                     ARQEKPDLILLDLILPGMDGFEILEKIKEEPENSIIPVIILSNLGQREDIEKGFDLGA
                     DDYLIKAHFTPDEIVERVRAPFRKPANA"
BASE COUNT          315 a          202 c          249 g          258 t
ORIGIN      
        1 tttatcactc agaacattat tgaggctcat cggggcaaga tctggtttaa atctaaggag
       61 aatgagggaa caaccttcta tattaagctt ccctattgaa tttgcaaagc agattttcct
      121 tgttataatt acattagtct atgaagaaga tcttaataat agaagacaac gaggagatca
      181 tgggtcttct cagccagaag atatcggacg ttggctatca ggtgattgag gcccgagacg
      241 gcgaagaggg tctgaggtta ttacgatcca atcagccgga tctggctctg ctagatatta
      301 ttctgcccaa tattgacggc ctcgagcttt taaagagaaa gcaagctctg ccagagacga
      361 aggacattcc ggtggtagtt atctctaatt ccggagctcc ggccgagatc agccaggctc
      421 agcgcttggg cgcagttgac tggatcacta agacagagtt tagtcttcag gagacgatag
      481 acaaaataga aaaaattatt aaagaatcaa gataattatc tttaaaatgc ctaactctcc
      541 taaaaaaata ctaattatcg aagacgacgt tttccttaat gagctgatgg ccaaaaagct
      601 agttgaagag ggctttgacg tgattaaggc ggttaacggc gaagaagggc tagagatggc
      661 ccggcaagaa aagccggatt tgattctact ggacttaatc ctaccgggca tggatggctt
      721 tgaaatttta gagaagatca aagaagagcc ggagaacagc attatcccgg taattatcct
      781 gtccaatctt ggtcagcgag aagatattga aaagggcttc gacctgggag ccgacgacta
      841 tttgattaaa gctcacttta cgccggacga aattgttgaa cgagttcggg cccccttccg
      901 taagccggcc aatgcctgag cttccagaag tcgagacgat ggttcgcgga cttcgttctc
      961 gagtcaaaag aaaaacaatt actaatcttt gggcggatga aaagaagata attcatcatc
     1021 cgtc
//
LOCUS       DAOOIE010000185         1022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_126994_length_1022_cov_3.410548, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000185 DAOOIE010000000
VERSION     DAOOIE010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126994_length_1022_cov_3.410548"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1022
                     /locus_tag="PK131_03605"
     CDS             <1..>1022
                     /locus_tag="PK131_03605"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HPD45238.1"
                     /translation="TFLSLAARSAKFSSGLIDIGDLFFFITWILIFVLATIISQKRKK
                     INLPLINLGIILVLINLLLTFYPSLKLDLTQDKVHSLSPISKEIVRNLDDIVNIKVLM
                     SPDLPVEVKPATDKLKAILAEFNRTNRRKFKVAYLDPAKDENAQAMAASLGIRPIQFN
                     QIKKDKLELQNAYLAVVIVYGQKQMVINLDLQNMEYLIISSIKKLINEKSPTVALFSE
                     NNNDLQYFRQYLSKDYTVINADLFSKNELPPADTLIIAGLSKKLDDKNLNKIKEWLKT
                     QKGLIVFLDRIGVDENMISQRYDSTGLESLLAENGIKIEDKLVLDGSAGVATFNTVDG
                     RILVQN"
BASE COUNT          351 a          168 c          174 g          329 t
ORIGIN      
        1 tactttttta agtttggcgg ctagaagtgc caaattctct tctggtttaa ttgatatcgg
       61 cgatctattt ttctttatta cttggatatt aatctttgtt ttagcgacga ttatcagtca
      121 aaaaagaaaa aaaataaatt tacctttgat taatttagga attattttag ttctaattaa
      181 cctcctgtta accttttatc cttcattaaa attagattta actcaagata aggtccattc
      241 tttatctccg atttcaaaag aaattgtccg taatttagat gatatagtca atattaaagt
      301 tttaatgagc cctgacctgc cggtggaagt caagccggct accgataaat taaaggcaat
      361 attagccgaa tttaatcgaa ccaatcgccg taaatttaag gtggcttacc ttgacccggc
      421 taaagatgaa aatgcccagg caatggcggc aagtctaggt attcgaccaa ttcaatttaa
      481 tcaaattaaa aaggataaat tagagcttca aaatgcctat ctggcagtag ttattgtcta
      541 tggtcaaaag caaatggtga ttaatctcga cttacaaaac atggaatatt taattatttc
      601 cagtattaaa aaactaataa atgaaaaatc tcccactgta gctttatttt cggaaaataa
      661 taatgacctt cagtacttca gacaatatct tagcaaggat tatactgtta ttaatgccga
      721 tttatttagt aaaaacgagc taccaccggc cgatacttta atcatcgctg gtttatcaaa
      781 aaaattagat gacaaaaatc tcaataaaat aaaagaatgg ctgaagaccc aaaagggatt
      841 aattgttttt cttgatcgga ttggtgttga cgaaaacatg attagtcagc ggtatgattc
      901 aaccggttta gaatcacttt tagccgaaaa tggaatcaaa atcgaggaca aattagtttt
      961 agacggaagt gccggcgtag ccacttttaa cactgttgac ggtcgaattt tggttcaaaa
     1021 cc
//
LOCUS       DAOOIE010000186         1015 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_128500_length_1015_cov_2.639583, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000186 DAOOIE010000000
VERSION     DAOOIE010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1015
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128500_length_1015_cov_2.639583"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..405
                     /locus_tag="PK131_03610"
     CDS             34..405
                     /locus_tag="PK131_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45239.1"
                     /translation="MNELQNLINAHYQRLDNLSRELKQQKEMLNDLLEKDEEFVQAEE
                     EAKKTAKVKALAKKKLLLRPEARALVEKIKDNQFQIKEIKLALSDYLAQYMREAGTTQ
                     IAAPDGSSYRIVYTARLVKSE"
     gene            431..715
                     /gene="trxA"
                     /locus_tag="PK131_03615"
     CDS             431..715
                     /gene="trxA"
                     /locus_tag="PK131_03615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPD45240.1"
                     /translation="MTIINKSGVTLVDFWAPWCGPCRMLMPIMDELEKELAGKVNFQR
                     INIDEEPELANQYNIKSIPMVLVFVNGELKEIISNFRQKEDYLEAIEKYI"
     gene            750..>1015
                     /locus_tag="PK131_03620"
     CDS             750..>1015
                     /locus_tag="PK131_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPD45241.1"
                     /translation="MLVDEVQITIKAGDGGDGLVHFYSDRFRPHGGPDGGNGGRGGDV
                     YFEAVNDILRLKRFRAGKFYEAPKGGNGGPNKRAGADGQDLVLT"
BASE COUNT          326 a          139 c          242 g          308 t
ORIGIN      
        1 gaagaagatt ttataaattg ttaaagtgag acgatgaatg aattacaaaa tttaattaat
       61 gctcattatc aacgtttaga taatctttcc cgggagttaa aacagcagaa ggaaatgttg
      121 aatgacttac tggaaaaaga tgaagaattt gttcaggccg aagaggaagc caaaaaaacg
      181 gctaaagtta aagctttggc caaaaaaaaa ttattgcttc gacccgaagc cagggcttta
      241 gtggagaaga ttaaggataa tcagtttcag ataaaagaaa ttaaactagc tttgtccgat
      301 tatttggctc agtatatgcg tgaggcgggg acaactcaga ttgccgctcc tgatggttcg
      361 tcttaccgaa ttgtttatac ggctagattg gtgaaaagtg aataatgaag aataatgtta
      421 taatctaaaa atgacaataa ttaataaatc aggtgtaact cttgttgatt tttgggcgcc
      481 atggtgtggt ccatgccgaa tgttaatgcc aattatggat gagttagaga aggaattagc
      541 tggtaaagtt aattttcagc gaattaatat tgacgaagaa cccgaattgg ctaatcaata
      601 taatattaaa agcattccaa tggttttggt ttttgtaaat ggggaactga aagaaattat
      661 atcgaatttt cgtcagaagg aagattatct tgaagcgatt gaaaagtata tttgattttt
      721 tttactaatt actggttata cattaaggga tgttagttga tgaggttcaa ataaccatta
      781 aggctggtga tggcggcgat ggcttagtcc atttttattc tgatcgattt cgaccccatg
      841 gtggtcctga tggcggcaac ggcggccgcg gcggcgatgt ttattttgaa gctgttaatg
      901 atattttgcg gctaaaacga tttcgagccg ggaagtttta tgaagcgcct aagggtggaa
      961 atggcggtcc caataaacgt gccggtgctg atggccagga tttagtttta actat
//
LOCUS       DAOOIE010000187         1010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_129668_length_1010_cov_1.894241, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000187 DAOOIE010000000
VERSION     DAOOIE010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129668_length_1010_cov_1.894241"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="PK131_03625"
     CDS             complement(<1..203)
                     /locus_tag="PK131_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45242.1"
                     /translation="MIFLVTLTIGAFFFLVISGQVEWANIIESLQLIRPWQFALLVLL
                     TLGIQGMATLAWQETLRHIGYRLP"
     gene            complement(275..541)
                     /locus_tag="PK131_03630"
     CDS             complement(275..541)
                     /locus_tag="PK131_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45243.1"
                     /translation="MPQEDLDEKNLIGQVVHYFDQIGVAVISLSKTLKVGDQIRFVGG
                     ETDFIQEVISMEVDHEKIDQAKAKQEVGMKVDQRVRPGYRVYKA"
     gene            complement(644..715)
                     /locus_tag="PK131_03635"
     tRNA            complement(644..715)
                     /locus_tag="PK131_03635"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(680..682),aa:Arg,seq:ccg)
     gene            complement(701..>1010)
                     /locus_tag="PK131_03640"
     CDS             complement(701..>1010)
                     /locus_tag="PK131_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45244.1"
                     /translation="LHHLPEVAAARVLSEAVRVVKPGGRIVITVWQPDRISFWQKLNR
                     NSFLRQWGDRALIYYHFFSLRDLKRVVLRSGALIEEAGFLKRGRRRNCFIVAYRAPIA
                     "
BASE COUNT          292 a          264 c          192 g          262 t
ORIGIN      
        1 ggaagacgat agccaatgtg gcgaagagtt tcctgccagg ccagagttgc catcccctgg
       61 atccccaggg tcagcaaaac cagcaacgca aattgccagg gtctaatcag ctggagcgat
      121 tcaataatat tagcccactc aacctggccg ctaatcacca agaaaaagaa agctccgatc
      181 gtcagcgtta ctaaaaaaat caagaagttt ttgaatgaca taatcgacaa agaaaaagca
      241 ccaaaaacat tgtttttagt gcattttcat aaaatcaagc cttgtaaacg cggtagcctg
      301 gtctaacccg ctggtcgacc ttcataccca cctcctgctt ggctttagcc tggtcaatct
      361 tttcgtggtc aacctccatc gagattacct cttggataaa gtcagtttct ccgccaacaa
      421 acctaatctg atcgccaacc tttagggtct tgctcaagct gatcacggcc actccgatct
      481 gatcaaaata atgaacgact tggccgatta agtttttttc gtccaaatct tcttgaggca
      541 taaaattaaa gataattttt aataattttg acctttatgg gtaaacccat tgctcttatt
      601 ctagcaaaat tatctctttt ttccagcctc agaaactagg tagtaccccc aaaaggaatc
      661 gaaccttcat cccggcctcc ggagggccgt gctttatccg ttaagctatg ggggcgcgat
      721 aagcaacaat aaagcagtta cggcgccgac ctctttttaa aaaacctgct tcttctatca
      781 gagcccctga tctcagaacc actcttttta aatctctcaa gctaaaaaaa tggtagtaga
      841 ttaaggcccg atcgccccat tgcctcaaaa aagaatttct atttaacttt tgccaaaagc
      901 taatacggtc cggctgccag acagtaatta ctatccggcc gcctggttta accacccgaa
      961 cggcttcgct taaaaccctg gccgccgcta cctcggggag gtgatgcagc
//
LOCUS       DAOOIE010000188         1009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_129777_length_1009_cov_3.381551, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000188 DAOOIE010000000
VERSION     DAOOIE010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129777_length_1009_cov_3.381551"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..265
                     /locus_tag="PK131_03645"
     tRNA            195..265
                     /locus_tag="PK131_03645"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:227..229,aa:Cys,seq:gca)
     gene            complement(356..784)
                     /locus_tag="PK131_03650"
     CDS             complement(356..784)
                     /locus_tag="PK131_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45245.1"
                     /translation="MERIEIDLSLFDNRRKEILETILKAGNNNYRQALNELGIIPLVT
                     PKARFRLNPVTCYQYVLGLDSFNRLQQLLDNGQFYQPGTGIIAVYYTDDLKPEKVNHL
                     GKMKNKQEVVSKFGKLGLFQHNITDVPIIYGNRVTFIKEP"
     gene            815..>1009
                     /locus_tag="PK131_03655"
     CDS             815..>1009
                     /locus_tag="PK131_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018061438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPD45246.1"
                     /translation="MGLRVIVAQPHGFCAGVSRAIKIVKDTAQKYPGKTIYLLGEIVH
                     NRHVVNDLKKNYRVKIVNSTN"
BASE COUNT          291 a          180 c          204 g          334 t
ORIGIN      
        1 gccgtttcta ttggcataat ggttttgacg accgacgttc taattactga tattccggag
       61 gaaccgaaag cacctatgcc cggggctgga atggaaggaa tgatgtaaaa aatacccccg
      121 ctaatgcggg ggttaaattt ttgaggccgt acttcgaaat ggtctggata tttgttaaaa
      181 tacctttatc ttttgacccg gtagtcaagc ggttagacaa cggtctgcaa aaccgtttag
      241 atgggttcaa ctcccatccg ggtctcttaa atttatttgg aattcttttt cctttttcat
      301 tccccgataa actatttagt ttcaattaaa aaacttcaac ggtctgaggg ttgttttatg
      361 gttctttaat gaaagtaact cgattgccgt aaataatggg aacatcagta atattgtgtt
      421 gaaataagcc aagtttgcca aatttagaaa ctacctcctg tttatttttc atttttccca
      481 aatgattaac cttttctggt ttgagatcgt cagtataata gacagcaata attccagtcc
      541 ccggttggta aaattggcca ttatcgagaa gttgttggag gcgattaaat gaatctaaac
      601 ctaggacata ttggtaacag gttactgggt ttagtcggaa tcgagctttg ggggttacca
      661 ggggaattat tcccaattca ttaagagcct gtcgataatt gttgttgcca gccttaagaa
      721 tagtttctaa aatttctttt cgccgattat caaaaagact tagatcgatt tctattcgct
      781 ccacggcgat attataacag ctataatatt ctatatgggt ttaagagtaa tagtagctca
      841 accccatggc ttttgtgctg gtgtttcccg ggccattaaa atcgttaaag atactgccca
      901 gaaatatccc ggcaaaacaa tttatctttt gggagaaatt gttcataatc gccatgtcgt
      961 gaatgatttg aagaaaaatt atcgggttaa aattgtcaat tctactaat
//
LOCUS       DAOOIE010000189         1007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Woesebacteria bacterium isolate
            AMR_MDS_2787 NODE_130211_length_1007_cov_3.097689, whole genome
            shotgun sequence.
ACCESSION   DAOOIE010000189 DAOOIE010000000
VERSION     DAOOIE010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864862
            Sequence Read Archive: SRR5007117
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Woesebacteria bacterium (wastewater metagenome)
  ORGANISM  Candidatus Woesebacteria bacterium
            Bacteria; Candidatus Woeseibacteriota.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 09:17:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 732
            CDSs (total)                      :: 698
            Genes (coding)                    :: 694
            CDSs (with protein)               :: 694
            Genes (RNA)                       :: 34
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 31
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 1 of 4
            Pseudo Genes (incomplete)         :: 3 of 4
            Pseudo Genes (internal stop)      :: 1 of 4
            Pseudo Genes (multiple problems)  :: 1 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Candidatus Woesebacteria bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_130211_length_1007_cov_3.097689"
                     /isolate="AMR_MDS_2787"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2026804"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..>1007)
                     /locus_tag="PK131_03660"
     CDS             complement(128..>1007)
                     /locus_tag="PK131_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPD45247.1"
                     /translation="LGLGLFFLGRFLNPKQLSAYQWPIFIFSILLLISPLVIGNFIRG
                     SRRWLSLGPINLQPSEITKPLLMIFIATSSQPLFHLAAVLLIALEPDLGSALSVLILL
                     TPVILTNRRILKISLLILIIGLVLSPFIWQHVLKDYQRQRLTTFINPQTNPLDGGYHV
                     IQSKIAIGSGGFWGKGYKKGTQGQLLFLPEKHTDFIFAATAEELGLAAVILILIAYFL
                     LSSSLLNHAYSLSDNRPLFIFNLGIFFLIWFQFFVNIGMNLGIMPVTGIPLPFLSVGT
                     SSLISFLFSLGICFSD"
BASE COUNT          394 a          151 c          212 g          250 t
ORIGIN      
        1 atttgatcag aggatgtttg actagttttg ggaacggtca agatctgtcc ctcttcaact
       61 agatattgac tacctgctag agaaatcacg gcatatttca tcatggcatt ataacatagt
      121 taaataatta atcagagaaa caaataccaa gagagaaaag aaaagagatt aaagatgagg
      181 tgccaacaga gagaaaagga agaggaatgc cggttaccgg cataatacct agattcatcc
      241 caatgttgac aaagaattga aaccaaatta gaaaaaaaat acctaaattg aaaataaaca
      301 aaggccgatt gtctgacaaa ctgtaagcat gattaagcaa agaagaagat agtaaaaaat
      361 aagcaattag gattaaaatt acagctgcca atcctaattc ttcagcagta gcggcaaaga
      421 taaaatccgt atgtttctcc ggcagaaaaa gtaattgtcc ttgggttccc tttttataac
      481 ccttccccca gaaacctccc gaaccaatag caattttaga ttgaataaca tgataacctc
      541 catctagagg attagtttgg ggattaatga aagttgttaa tctttgacgt tggtaatctt
      601 ttaagacatg ttgccaaatg aatggagata aaactaaacc aataattaaa attaagaggg
      661 aaattttaag tattcggcga ttagtaagaa ttaccggtgt taaaagaatt aaaactgata
      721 gggctgaacc taaatcaggt tcgagagcaa ttaaaaggac agctgccagg tgaaagagtg
      781 gttgagaaga ggtagcaatg aatatcatta acaggggttt ggtaatttct gaaggttgaa
      841 gattaattgg acctaaacta agccagcggc gagaaccacg aataaaattg ccgatgacta
      901 gtggactgat taataagagg atggagaaaa taaaaattgg ccattgatag gcagagagtt
      961 gtttagggtt taagaaacgg ccaagaaaaa acaagcctaa gcctaaa
//
